LOCUS       DATIMH010000001         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3753988, whole genome shotgun sequence.
ACCESSION   DATIMH010000001 DATIMH010000000
VERSION     DATIMH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3753988"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1154)
                     /locus_tag="VLI90_00005"
     CDS             complement(30..1154)
                     /locus_tag="VLI90_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12609.1"
                     /translation="MNGRFGLWIVRIALTSCLAGAVGCAEYNRYPTHAESDSLADVIQ
                     LTTGFDRAGEAYFSHDMRWIVFQATPPGEQQYQMYVAKVRYGNRSGDARGEIVGIERP
                     TRITPANSRNTCGFFSPDGVSLIFASTAGKENPNEPSAGYQRGGSNYRWSFPAGMEIF
                     RVDGWEGAVSMSDPAKGIDLAQHAMTDNDAYDAECAYSPDGKFICFTSNRGGDLDVYV
                     MHADGSHVVQITKTPGYDGGPFFSPDGKSLVYRADRKGKDLLQIYVGDLNFDRNGEIT
                     GIKAEHQLTDDANVNWGPYWHPDGQHVIYATSKHGHTNYELYLMRRDGTRKTRVTFTN
                     GADILPVFSPDGKYLMWTSKRTADQTTQIFLGKFEFAQGT"
     gene            1549..2202
                     /locus_tag="VLI90_00010"
     CDS             1549..2202
                     /locus_tag="VLI90_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12610.1"
                     /translation="MSLDGKLRKRLERALAVVDDHGTDGTRLIDDAHRLWRRVQKFLN
                     LNLIPNGPNGADLDALELASYALQLPFRQNKVPTVGKLGRTNLKERAEQAAEMLLGLL
                     GDDVDESLLDRATRLLHELPHRSPMLDEARLLADAVNLEDFGVTGLIQQAVQLSRQGE
                     GLMQVAEGAEKREQYGYWNARLKDGFHFDPVRQMALRRLENARTAAKLLAEEMAEDV"
BASE COUNT          398 a          672 c          730 g          436 t
ORIGIN      
        1 gctgcgattg cgtccggttc atcgcgaatt cacgtcccct gcgcgaactc gaacttcccc
       61 aggaagatct gcgtcgtttg atccgccgtc cgcttgctcg tccacatcag gtattttccg
      121 tccggcgaaa acactggaag gatgtcggcg ccgttggtga acgtcacgcg ggtcttgcgc
      181 gtgccgtcgc ggcgcatcag gtacaattcg tagttcgtgt ggccgtgctt gctggtggcg
      241 tagatgacgt gctggccgtc ggggtgccaa tatggacccc agttcacgtt ggcatcgtca
      301 gtcagctgat gctcggcttt gattccggtg atctcgccgt tgcgatcgaa gttcagatcg
      361 ccgacataga tctgcagcag atccttccct tttcgatcag cgcgatagac caggctcttg
      421 ccgtccggcg agaagaacgg cccgccgtcg tagccgggcg tctttgtaat ctgcaccaca
      481 tgcgatccgt cggcgtgcat gacgtacacg tccaggtccc cgccgcggtt ggacgtgaag
      541 cagatgaatt tgccgtcggg tgagtacgcg cactctgcgt cgtacgcgtc gttgtcggtc
      601 atcgcgtgct gggcaaggtc gatccccttc gccggatcgg acatcgagac ggccccttcc
      661 cagccatcga cgcggaagat ttccatgccg gcggggaagc tccagcggta gttcgatccg
      721 ccgcgctggt aacccgcgct gggctcattc ggattttcct tacccgcggt tgacgcgaaa
      781 atgagcgaca caccgtcggg cgaaaagaag ccgcaggtat tgcgcgagtt ggccggcgtg
      841 atacgggtgg ggcgttcgat gccgacgatc tcgccgcgcg catcgccgga gcgattgccg
      901 taccgcacct tggccacgta catctggtat tgctgctcgc ccggtggggt tgcctggaaa
      961 acgatccagc gcatgtcgtg cgaaaagtag gcctccccgg cgcgatcgaa gccggtggta
     1021 agctggatga cgtcggcaag cgagtcagac tcggcgtgcg ttggatagcg attgtattcc
     1081 gcgcaaccga ccgcgccggc aaggcagctg gttaacgcga tcctcacgat ccacaagcca
     1141 aaacgaccgt tcattcgatg acctcattcg tatatcgtcg cgcccgcggt gcgcggcatg
     1201 agtggatctc ggcgcgcgtt gcacccgcgc gcgggcgcgc gtgcggatcg ctttttgcga
     1261 atttagtgtt cgtaaatgga cgcgggtttc gatattacta gcaccggcgc gtccggacgt
     1321 gggtggccag agaatttatg cgttggccgc gcgccagtgg gtccagatcc aaaacagatc
     1381 gacggccgcc gccgcacaac cgccgcgctt ggtgtacttc ggacgttcac ttggtcgaaa
     1441 acgctctcgg gctcgttgcc gtgggtgccg ttggtccgcg tgacgtgcgc tcgcgggcgg
     1501 cgcgttgttg tgttcagccg agacctgacg gttagttcgg actccgcgat gtcgttagat
     1561 gggaagttgc gaaagcgcct ggaacgcgcg ctggccgtgg tcgatgatca cggcacggat
     1621 ggcacgcgcc tcatcgacga tgcgcaccgc ctgtggcggc gcgtccaaaa attcctcaac
     1681 ctgaacctga tccccaacgg gccgaacggc gcggatctgg acgcgctgga actggcgtcc
     1741 tatgcgctgc agttgccctt ccgccagaac aaggtgccga cggtcggcaa gctcggtcgc
     1801 accaacctga aggagcgcgc cgagcaggcc gccgagatgc tgctgggctt gctgggcgat
     1861 gacgtggatg aatcgctgct ggaccgagcg accagactgc tgcacgaatt gccgcaccgc
     1921 tcgccgatgc tggatgaagc ccgcctgctg gccgacgcgg tgaacctcga agacttcggc
     1981 gtcacaggcc tcatccaaca agcggtgcaa ctgtctcgtc aaggcgaagg gctgatgcag
     2041 gtggccgagg gcgcggagaa gcgcgagcag tacggctatt ggaacgcccg cctgaaagac
     2101 ggatttcact tcgatcccgt ccgccagatg gccctgcgcc gactggaaaa cgccagaacg
     2161 gcggcgaaac tgctggcgga ggaaatggcg gaagacgtgt gatggcgtga gtgcaccacc
     2221 acgccgggtt cggaac
//
LOCUS       DATIMH010000002         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1732754, whole genome shotgun sequence.
ACCESSION   DATIMH010000002 DATIMH010000000
VERSION     DATIMH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732754"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VLI90_00015"
     CDS             complement(<1..641)
                     /locus_tag="VLI90_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSV12611.1"
                     /translation="MSVLFSAVVGGCMVGVDYKRPKTDAPAQFAEGHNGPSTQPAVVV
                     DLTKWWKTFNDPELESLVDRAVQTNFDVQTAEANVRAARAQLGVEEANLFPTVDASGG
                     YTRSQSSLNASSGSITGTTSGTAGATGATGSSGSLSGFSVRRQRDLYQAGFDAGWEVD
                     VFGGTRRGIEAAASDLEAQVNARRNVLVSVTAEVARNYIQLRGLQRQLELTFN"
     gene            complement(712..1701)
                     /locus_tag="VLI90_00020"
     CDS             complement(712..1701)
                     /locus_tag="VLI90_00020"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSV12612.1"
                     /translation="MFTRYILPAVALFGVIFAVIFVHAGNKPVPASQPVADPAQAPFA
                     SYVAGAGIVESSTEEIPVGTLVPGVVSEIYKSIGERVRAGDPLFRIDSRDLEAELAVR
                     KADLVSAKANVSAQEATAADMQNQWKNAQAMGDARAMSAEELDRRRFAAQVAAAKLEQ
                     ANADVVSAEAHVKQTETELDRRIVRARVDGTLLQSKIHLGEYAQVGPLATPLMLLGNT
                     DVLNVRVDIDENDAWRVDTTRAAIAFVRGNRDLSTELHFVRIEPYVVPKRSLTGDSSE
                     RVDTRVLQVVYSFNPKSLPVFVGQQMDVFIEARPIGQATAYTRAPASGPAGGR"
     gene            complement(1849..2574)
                     /locus_tag="VLI90_00025"
     CDS             complement(1849..2574)
                     /locus_tag="VLI90_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV12613.1"
                     /translation="MAATQDDIAVRCRGVTKVYRTGGTEVHALRGIDLDVPTGELMML
                     VGPSGCGKTTLISVIAGILDQDDGECKVYGRDFKAMGDRARTTYRGQNIGFVFQAFNL
                     LPTLTAAENVAVPMLINGHPRRDALERAEESLRRVGLGDRMRSLPKQLSGGQQQRVAI
                     ARALVHSPKLIVCDEPTSALDHETGHKVMELLREVAVGADRALVIVTHDARIFEFADR
                     IAKMDDGRVVQVAESAREFVNGK"
     gene            complement(2605..>2961)
                     /locus_tag="VLI90_00030"
     CDS             complement(2605..>2961)
                     /locus_tag="VLI90_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV12614.1"
                     /translation="TIALGFVIGLLVAGQTFYTFVLDNLRPFAAIKAMGASNGMVLRM
                     LCVQVIVVAAIGYGIGLGAATVSGAILSRGGLAFQMAWQIPVFGAVAVLICCLAAGCL
                     GMLRVLRLEPAIVFKG"
BASE COUNT          521 a         1052 c          853 g          535 t
ORIGIN      
        1 ttgttgaacg tcagctcgag ctggcgctgc agcccgcgaa gctggatgta atttcgcgcg
       61 acttcagcgg tgaccgacac caggacgttc cgccgcgcgt tgacctgcgc ttccaggtcc
      121 gacgccgccg cctcgatccc ccggcgggtg ccgccgaaga catcgacctc ccaaccggcg
      181 tcgaaaccgg cttgatacag atcgcgctgc cggcgcacgc tgaaaccaga gagggatcca
      241 cttgaccccg ttgcaccggt ggcaccggca gtgccgctgg tcgtgccggt gatgcttccg
      301 ctgctggcat tcaagctgga ctgcgaccgc gtgtagccgc cggacgcatc gacggtggga
      361 aagaggttcg cctcttccac acccagctgc gcgcgggcgg ctcgcacgtt cgcttcggct
      421 gtctgcacgt cgaagttggt ttgcacggcg cgatcgacca gcgattcgag ctccggatcg
      481 ttgaacgtct tccaccactt cgtcaggtcc accacaacgg ccggctgggt gctcggtccg
      541 ttgtgcccct cggcaaactg cgccggggcg tccgtcttcg gccgcttata gtccacgccc
      601 accatgcacc caccaaccac ggccgagaag agaacggaca atgcgagatt ctttttcatt
      661 cctgcggtcc tcatgatcct gatgattcga ttgacaaaaa cggcggtaac tttaccgccc
      721 cccggctggc cctgacgccg gtgcacgagt ataggccgtc gcctgcccga tggggcgagc
      781 ttcgatgaac acatccatct gctgccccac aaataccggc agcgacttcg gattgaaact
      841 gtagacgacc tgcagcacgc gcgtatcgac gcgctccgag ctgtcgccgg tcaacgatcg
      901 cttgggcacg acgtacggct cgatccgcac gaagtgcagc tcagtgctaa gatcgcgatt
      961 gccacggacg aatgcgatcg ccgctcgagt cgtgtccacg cgccaagcat cgttttcgtc
     1021 gatgtccacg cgaacgttta agacgtcggt gttacccagc agcatcagcg gcgttgccag
     1081 cgggcccacc tgcgcatatt cacctaagtg aatcttgctt tgcagcaacg tgccatcgac
     1141 gcgggcgcgg acgatccggc gatccagctc cgtctccgtc tgcttcacgt gcgcctcggc
     1201 cgacacgaca tcggcgttcg cctgctccaa cttcgccgcc gccacctggg cggcgaatcg
     1261 ccggcggtcc aactcttcag cactcatcgc ccgggcatcg cccatcgctt gagcgttctt
     1321 ccactgattc tgcatgtccg ccgccgtcgc ctcctgtgcc gagacgttgg ccttggccga
     1381 cacgaggtct gctttgcgaa ccgccagctc cgcttccaga tcgcgcgagt cgatgcggaa
     1441 cagcgggtcc ccggcgcgca cgcgctcgcc gatcgatttg taaatctcgc tcaccacgcc
     1501 cggcaccagc gtgccgacgg ggatctcctc ggtgctcgat tccacgatgc ccgcgccggc
     1561 cacgtacgac gcgaacggcg cctgcgccgg atccgccacg ggttgcgagg ccggcaccgg
     1621 cttgttcccc gcgtgaacga agatcacggc gaagatcacg ccaaacagcg caacagcggg
     1681 aaggatgtat cgggtgaaca tgacggctcc tgtctctttc tggtcccctc gcccggtacg
     1741 ccgggagagg gttagggtga gggtgatttt gttcttcgag aatcactcga tgttcgaatc
     1801 accctcaccc ccgccctctc cgcacgcgta cgtgggagag ggggtaagtc actttccatt
     1861 cacaaactct ctcgcgctct ccgccacctg caccactcgc ccatcgtcca tcttcgcaat
     1921 gcgatcggcg aactcgaata tgcgggcatc gtgcgtcacg atcaccagcg cccggtccgc
     1981 tcccaccgcg acctcgcgca gcagctccat caccttgtgg ccggtctcgt gatccagcgc
     2041 gctggtcggt tcatcgcaga cgatcagctt cggcgaatgc accagcgccc gcgcgatcgc
     2101 gacccgctgc tgctgtccgc cggacaattg cttcggcaac gaccgcatgc gatcgcccag
     2161 tcccacgcgg cgaagcgatt cctcggcgcg ctccagcgca tctcgccgcg gatgcccgtt
     2221 gatcagcatg ggcaccgcga cgttctcggc cgccgtcagc gtgggcagca ggttgaaggc
     2281 ttggaagacg aacccgatgt tttgcccgcg gtacgtcgtg cgggcgcggt cgcccatcgc
     2341 cttaaaatcc cggccataga ctttgcactc gccgtcgtcc tgatcgagga tgccggcgat
     2401 cacgctaatg agggtcgtct ttccgcatcc gctgggaccg accagcatca tcaactcgcc
     2461 cgtcggcacg tccagatcga tcccgcgcag cgcgtgcacc tcggtcccgc cggtgcggta
     2521 caccttggtg accccgcggc agcgcacggc gatgtcgtcc tgggtcgcag ccatagccga
     2581 ttcttccaac gccaacgtca tgacttatcc tttgaacacg atcgccggtt ccagccgcag
     2641 cacgcgcaac attcccaggc atcccgcggc caggcagcag atcagcaccg ccacggcgcc
     2701 gaagaccggg atttgccacg ccatctgaaa cgcgaggcct ccgcgcgaaa gaatcgcgcc
     2761 agataccgtc gccgccccca aaccaattcc gtaaccgatg gcggcgacga cgatcacctg
     2821 cacgcacagc atccgcagca ccatgccatt gctcgcgccc atcgccttga tggcggcgaa
     2881 cgggcgcagg ttgtccagga cgaacgtgta aaacgtctgc cccgccacca gcaggccgat
     2941 gacgaacccc agcgcgatcg t
//
LOCUS       DATIMH010000003         5905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3465337, whole genome shotgun sequence.
ACCESSION   DATIMH010000003 DATIMH010000000
VERSION     DATIMH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3465337"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /gene="flgL"
                     /locus_tag="VLI90_00035"
     CDS             complement(<1..560)
                     /gene="flgL"
                     /locus_tag="VLI90_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HSV12615.1"
                     /translation="MAILPLQLARVSNLLRSDLATQAMSQQQQLLLQVQNEIATGKRV
                     NTPSDDPSAAATIQQLQKTLDNRAGWTDNINAAKSQLGAVDSTLSDLNDLLQQAQQIA
                     SQNVSSTVSADQRASAAAVVDSLYDQAMTLANKQFNGAYLFGGDLSTDPPYVQATGGV
                     QFVGSTTTLQNTVDQGKMLGFQVSGAA"
     gene            complement(617..2323)
                     /gene="flgK"
                     /locus_tag="VLI90_00040"
     CDS             complement(617..2323)
                     /gene="flgK"
                     /locus_tag="VLI90_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HSV12616.1"
                     /translation="MSLLGALNAGKTALAVQQAAIQVTGNNIANAGNADYTRETVQLA
                     PSAAQEIQSGVFLGTGVDLTGVQRQVDDALLSRMRQSMSDNSAAGSTQQWLSQVESVF
                     NALSGQDLSSHMSTFFNDWSTLAGKPTDIGQRQIVLQDGQDLAGYLQNLKSQLGGLQS
                     GIDDQIKGQASAANDMAQKVADLNRQIIVTEGGTAGQANGLRDQRDAVLKQLSQLMNI
                     KTLEQPNGTVNVYVGSEPLVDGDTNHGVQLKMVPDATTGALVPNLTFKSNNGMIPVTS
                     GQLGGLLDARNQITGYSGQVDTIAHNLIFELNKLHASGQGLEGFSSVTATNQVLDPTV
                     PLNQPAAGLPFTPVNGSLVVHVKQKATGLVTSTLVNIDLDGLGGNDTTLNSLAANLNG
                     ISGITANTNGNLLNIAASSSAVEISFSQDTSGALAALGINTFFSGKSATDIALNQTIA
                     GQPSLLAVAMNGNKADNQTALAISQLETKTIPGLNGQSLKDNYQAVVNQIGTSAAAAK
                     QNAQATQVVQDTLTSQQQALSGVSMDEEAVNLVRQQRAFQAASRIVSAVDDMMKTIIQ
                     MV"
     gene            complement(2351..2725)
                     /locus_tag="VLI90_00045"
     CDS             complement(2351..2725)
                     /locus_tag="VLI90_00045"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HSV12617.1"
                     /translation="MAARTYRELVAWQRAMDFAVEVYRATRQFPREELYGLTSQVRRA
                     AVSVPSNIAEGQGRGVGAEFAHHLRIGQNSLEEPETPILLGERPGYLQCADVNALMDI
                     AGDVGRLTRGLHASVCPNDSSH"
     gene            complement(2766..3281)
                     /gene="flgN"
                     /locus_tag="VLI90_00050"
     CDS             complement(2766..3281)
                     /gene="flgN"
                     /locus_tag="VLI90_00050"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HSV12618.1"
                     /translation="MNPANSNANIRQIVDLEAVLQQLILEHRKLLKHIDAQQAAMKTL
                     NLRAMDEATNQQEAARLRIATAEHKRRTLTIQIAKLLRVSGEPKIPQLAEFFPHRKPV
                     LMQLRTELLKAMGEIAAKNHIAGKLAGAVLGHLNTAVRLLAGAVEHAGLYTKHGVPQM
                     TARIGVMEAVG"
     gene            complement(3278..3664)
                     /locus_tag="VLI90_00055"
     CDS             complement(3278..3664)
                     /locus_tag="VLI90_00055"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HSV12619.1"
                     /translation="MTAQPIQFDKRYSGMKPPAPVDPQHAKLVAATQKWVAQTFYGAM
                     LKQMRNSPFKSEMFEGGRGGQAFNEMFDQHMAEHMARGAGSKLVKAIVRKTEAAAAYR
                     KQAAAKGQGVGPVSASSASSGAKGGK"
     gene            complement(3756..4853)
                     /locus_tag="VLI90_00060"
     CDS             complement(3756..4853)
                     /locus_tag="VLI90_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HSV12620.1"
                     /translation="MRRAILASILLTFAGAHSALAVKVADITRLNGARTNVLSGLGLV
                     VGLKGTGDGGAYAAAIRPLAALLAKYADPTAVSELQNAANVAVVQVMATVPANGVRNG
                     DHLNVRVLSTGAATSLHSGHLFITPMLGPTAQPYIPRDADGNPLRPIPFALAEGPVVI
                     EDPTSPTAGIVEGGAVMEVDLPARYIDNAGRFTLILDDPAASWTTASTIAKLINEAGD
                     TGETIATALDPKNIIVTIPTSERERPDSFISAILRLPVPMLASEAKVQINEKTGTMIV
                     TGDVEISPVVISHKGLTITTINPQPPPTFRNPQVSTKEVIPLDTTNQGGAKLQDLANA
                     FDALHVPAEDRITIVKELYKTGKLHAKLEVE"
     gene            complement(4969..5721)
                     /locus_tag="VLI90_00065"
     CDS             complement(4969..5721)
                     /locus_tag="VLI90_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HSV12621.1"
                     /translation="MAKPAKPVRIIALSSIALGTAGAAQHEQRVAPPASPPPITTQAL
                     MQGSGGSLLQASGPLQAEPMNSKTPSYNLYAVAEPEPKVLKKHDLINVIVREESQSSS
                     KGTTDLKRNADLDAKVDSFIKLNPSSFTIQGGTLKNPPEIKMSGTRNFKGDATAERTD
                     TVVVRLEGEVLDVKPNGTMVVQARKHIKTEDEEQTFILTGVCRVQDVDASNSVLSTDL
                     HDLDLQKITTGAVRDTTKRGLVGRLLDFLNPF"
     gene            5720..>5905
                     /locus_tag="VLI90_00070"
     CDS             5720..>5905
                     /locus_tag="VLI90_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12622.1"
                     /translation="MGTSKFEKTVSSSRAGGTPPESKNLAEKYVKDRSRERIAPPPPL
                     AASQTDKPRSLICEAASG"
BASE COUNT          912 a         1927 c         1798 g         1268 t
ORIGIN      
        1 gcggcgccgc tgacctggaa gccgagcatc ttgccctggt caacggtgtt ctgcagcgtc
       61 gtggtcgatc cgacgaactg cacgccgccg gtcgcctgca cgtacggcgg atcggtcgag
      121 aggtcgccgc cgaacaggta cgcgccgttg aactgcttgt tcgccagggt catcgcctgg
      181 tcgtacaacg aatcgacgac ggcggcggcg ctggcgcgct ggtcggccga caccgtggac
      241 gacacgttct gcgatgcgat ctgctgcgcc tgctggagca ggtcgttcaa atcgctcagc
      301 gtcgaatcga ccgcgcccag ctggctcttg gccgcgttga tgttgtcggt ccacccggcg
      361 cggttgtcga gcgtcttctg caactgctga atcgtcgccg ccgccgacgg atcatcgctg
      421 ggcgtgttga cgcgcttgcc ggtggcgatc tcattctgca cctgcagcag caactgctgc
      481 tgctgagaca tcgcctgcgt cgccagatcg ctgcgaagca gattagaaac ccgggcgagt
      541 tgtaggggca ggatagccat tgcgttcacc aagactctgg tcccctcgcc cggtacgccg
      601 ggcgaggggc aaggaatcac accatctgaa taatcgtctt catcatgtca tccaccgcgc
      661 tcacgattcg cgacgccgct tggaaggcgc gttgttgccg gaccaaattg accgcttctt
      721 catccatgct cacgccgctc agtgcttgct gctgcgacgt cagcgtgtcc tgcaccacct
      781 gcgtggcctg cgcgttttgc ttcgcggcgg cggcgctggt gccgatctgg ttcaccaccg
      841 cctgatagtt gtccttcagg ctctggccat tcaggcccgg gatcgtcttg gtttccaatt
      901 gcgagatggc caacgcagtc tggttgtccg ccttgttgcc gttcatcgcg acggccagca
      961 gcgacggctg gcccgcgatg gtctggttca gcgcaatgtc ggtggcgctc ttgccgctga
     1021 agaacgtgtt gatgcccagc gccgccagcg cgccgctggt gtcctggctg aagctgatct
     1081 ccaccgcact gctcgacgcg gcgatgttca gcaggttccc gttggtgttg gcggtgatcc
     1141 cgctgatgcc gttcagattc gccgccagtg aattcaaggt cgtgtcgttc ccgcccagcc
     1201 catccagatc gatgttcacc agtgtgctgg tgaccaatcc cgtcgccttc tgcttcacgt
     1261 ggacgaccag gctgccgttg accggcgtga acgggagccc cgccgccggt tggttcagcg
     1321 gcaccgttgg atcgagcacc tggttcgtcg cggtgacgct ggaaaatccc tccagcccct
     1381 gcccgctcgc gtgcagcttg ttcagctcga agatcaggtt gtgcgcgatc gtgtcgacct
     1441 gcccggagta accggtgatc tggttgcgcg catccagcaa tccgcccagc tgcccgctgg
     1501 tcacggggat catgccgttg ttcgacttga aggtcaggtt cggcaccagc gcgccggtgg
     1561 tcgcatccgg caccatcttc agctgcacgc cgtggttggt gtctccatcc accagcggct
     1621 cgctgccgac gtacacgttc accgtgccgt tgggttgctc cagcgtcttg atgttcatta
     1681 gctgcgacag ctgcttcagc accgcatcgc gctgatcgcg caggccgttc gcctgacccg
     1741 cggtgccgcc ttcggtcacg atgatctgtc gattcaggtc ggccaccttc tgcgccatgt
     1801 cattcgccgc actagcctgt cctttgatct ggtcatcgat cccgctctgc agtccgccga
     1861 gttggctctt cagattttgc agataccccg ccaggtcctg cccatcctgc agcacgattt
     1921 gccgctgacc gatgtcggtc ggcttgcccg ccaacgtgga ccagtcgttg aagaacgtgc
     1981 tcatgtgcga tgacagatcc tgcccgctga gcgcgttgaa gaccgattcg acttggctca
     2041 accactgctg cgtggacccg gcagcggagt tgtcgctcat gctctgccgc atgcgcgaca
     2101 gcagcgcatc gtccacctgc cgctgaacac ccgtcagatc aacgccggtg cccaggaaga
     2161 cgccgctttg aatctcctgc gccgccgacg gcgccagctg cacggtctcg cgcgtgtagt
     2221 cggcgttgcc ggcgttggcg atgttgttgc cggtgacctg aatggccgcc tgctgcaccg
     2281 ccagtgccgt cttgcccgcg ttgagtgctc ctaggagaga cataagttgc ttgttgctgg
     2341 ttgctagttg ctagtgggag gagtcgttgg gacagaccga agcgtgcagg ccgcgggtga
     2401 gacggccgac gtcgccggcg atgtccatga gtgcgttcac atcggcgcat tggagatagc
     2461 ccggtcgttc gccaagcaga attggtgttt ccggttcttc aagcgagttt tggccgatgc
     2521 gcaggtggtg cgcgaattcc gcgccgacgc cgcggccctg gccttcggcg atgtttgacg
     2581 gaacggagac tgcggcgcga cgcacttggc tcgtgaggcc gtacagttcc tcgcgcggga
     2641 actgccgcgt cgcgcgataa acttccactg cgaagtccat cgcccgttgc cacgccacca
     2701 gctctcgata ggttcgtgca gccatcgtct tgcctttcac cagcaactag caactagcaa
     2761 ctgattcatc ccaccgcttc catcacacca atccgcgccg tcatctgcgg cacgccgtgt
     2821 ttcgtgtaca agcccgcgtg ttctacggcg ccggctaaga gtctcaccgc ggtgttcaaa
     2881 tgtccgagta ccgcgccagc taactttccc gcgatgtgat ttttcgccgc gatctcgccc
     2941 atcgccttga gcagctccgt gcgcagctgc attagcaccg gcttgcggtg ggggaagaac
     3001 tcggccagct gcggaatttt gggttcgccg ctcacgcgca gcagcttggc gatctggatc
     3061 gtcagcgtcc gccgcttgtg ctcggccgtc gcgatgcgaa gacgagccgc ttcctgctgg
     3121 ttcgtcgctt cgtccatcgc gcgcaggttc agcgtcttca tcgccgcctg ctgggcgtcg
     3181 atgtgcttca gcagcttgcg atgctcgagg atcaactgct gaaggaccgc ttcgagatcg
     3241 acgatctgcc gaatgttcgc gttgctattc gccggattca tttgccgccc ttcgcgccgc
     3301 tcgaagcgct agaagcgctg accgggccca cgccctgccc cttcgccgcc gcctgcttgc
     3361 ggtacgccgc cgcggcttcg gtttttcgca cgatcgcctt caccagtttc gaccccgctc
     3421 cccgcgccat gtgctcggcc atgtgctggt cgaacatctc gttaaacgcc tgcccgccgc
     3481 ggccgccttc gaacatctcg ctcttgaacg ggctgttgcg catctgcttc agcatcgcgc
     3541 cgtagaacgt ctgggcgacc cacttctgcg tggcggcgac gagcttggcg tgctgaggat
     3601 ccaccggcgc cggcggcttc atgccgctgt agcgtttgtc gaattgaatt ggctgtgcag
     3661 tcatggtcgc gtctccgacg ctattcgtga cgcgtgaatc cgtgaatacg tgattgcgtc
     3721 ggtccgcctc ttctcacgta tttacgtaat cacgttcact cgacttccaa cttcgcgtgc
     3781 agcttccccg tcttgtacag ctctttcacg atcgtgatcc gatcctccgc cggcacgtgc
     3841 aacgcgtcga acgcgttcgc caggtcctgc agcttcgccc cgccctggtt cgtcgtgtcc
     3901 agcggaatga cttccttggt cgaaacctgc gggttgcgaa acgtcggcgg aggctgcggg
     3961 ttgatcgtcg taatcgtcag gcccttgtgg ctgatcacca ccgggctgat ctccacgtcg
     4021 ccagtcacga tcatcgtgcc ggtcttttcg ttgatctgca ccttcgcttc ggacgccagc
     4081 atcggcaccg gcaaacgcag gatggcggag atgaaactgt ccggccgctc gcgctcactg
     4141 gtggggattg tgacgatgat gtttttggga tcgagcgcgg tggcgatcgt ttcgccggtg
     4201 tcgccggctt cgttgatcaa cttggcgatc gtgctcgcgg tggtccagct ggcggcggga
     4261 tcgtccagga tcagcgtaaa cctgccggcg ttgtcgatgt atcgcgcggg cagatcgact
     4321 tccatcaccg ccccgccttc gacgatgccg gccgtggggc tggtcgggtc ttcgatgacg
     4381 accggccctt ccgccagcgc aaacggaatc ggccgcagcg gattgccgtc ggcatcgcgc
     4441 ggaatgtacg gctgggccgt gggcccgagc atcggcgtga tgaacaggtg accgctatga
     4501 aggctggtgg ccgccccggt gctgaggacg cgcacgttga gatgatcgcc gttgcgcaca
     4561 ccgttggccg gcacggtcgc catcacctgc acgaccgcca cgttggccgc gttctgcaat
     4621 tcgctcacgg cggtcggatc agcatacttc gccagcagcg ccgccagcgg tcgaatcgcc
     4681 gccgcatacg ccccaccgtc accggtgcct ttgagaccga cgaccagacc cagtccacta
     4741 agaacgttgg tgcgcgcgcc gttgaggcgg gtgatgtcgg caaccttcac cgccaaggcg
     4801 ctgtgagcgc cggcgaacgt cagaaggatg gatgcaagga tcgcacgtcg catgaaaagg
     4861 gctccgccct tggacaattg ctgtttgcca tcaaacgctg ggacacttct cctcaccagc
     4921 aacgagcaac cagcaacaag caactgattc tcttcccccc attcttcgtc agaacggatt
     4981 cagaaagtcc aataatctcc cgaccaatcc gcgtttggtc gtgtcgcgca ccgcgccggt
     5041 ggtgatcttc tgcaggtcca ggtcgtgcag gtcggtgctc aacacgctgt tgctggcatc
     5101 gacatcctgc acccggcaaa cgccggtcag gataaatgtc tgctcctcgt cctcggtctt
     5161 gatgtgcttg cgggcctgca cgaccattgt gccgttcggc ttcacgtcca gcacttcgcc
     5221 ttccaggcga acgaccaccg tgtcggtgcg ctcggccgtc gcgtcaccct tgaagttccg
     5281 cgtgccggac atcttgatct ccggcgggtt cttcagcgtg ccgccctgga tcgtgaagct
     5341 cgatgggttg agcttgatga acgaatcgac cttcgcgtcc aggtccgcgt tgcgcttcag
     5401 gtccgtggtc cccttcgacg acgactggct ctcctcgcgc acgatcacgt tgatcaggtc
     5461 gtgcttcttc agcaccttcg gttccggctc ggccaccgcg tagaggttgt acgacggcgt
     5521 cttcgagttc atcggctccg cctgcaacgg ccccgacgcc tgcagcagcg atccaccgct
     5581 gccctgcatc aacgcctgcg tggtgatcgg cggcggcgaa gccggcggcg ccacccgctg
     5641 ctcgtgctga gcggcgcccg cggtgccgag cgcgatggaa ctcaatgcga taatgcggac
     5701 tggctttgct ggcttggcca tgggtacatc caaattcgaa aagactgtgt catcctctag
     5761 ggcaggcggt actccgccgg agtcgaagaa tctggccgag aagtatgtga aggatcgatc
     5821 gcgcgagcga atcgcgccgc cgccgccgct tgcggcttcg cagacggaca agccgcggtc
     5881 attgatctgc gaagccgcaa gcggc
//
LOCUS       DATIMH010000004         1752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_578357, whole genome shotgun sequence.
ACCESSION   DATIMH010000004 DATIMH010000000
VERSION     DATIMH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578357"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VLI90_00075"
     CDS             complement(<1..506)
                     /locus_tag="VLI90_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HSV12623.1"
                     /translation="MPVMRPEKLAAPLERLDAGLAAMSPPLGAAARTAIERWRADAAA
                     LRFHLRKTNLTVPMTVILGGTGTGKSTLVNRIVGATVTATSFRRTFTSGPVAIVRDVK
                     LVPEGWLGVEHVIAQASDLPARGRADALIVVPLAAETASPAVLVDTPDLDGDQPVHHA
                     QADRAFRWA"
     gene            complement(537..1439)
                     /locus_tag="VLI90_00080"
     CDS             complement(537..1439)
                     /locus_tag="VLI90_00080"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSV12624.1"
                     /translation="MEQSLKNRLTFGPLMMGSLLLLLWLDSAAQQWTQRVRPGGVGGL
                     GLLVILVIILPLAIGEIAVLFTAERIAPYRRFAASGAAVLVLHAFLTQFTWFQPIAAS
                     TLAFIIVFVMLLAALTRAWDRETQGAIHRMAGTVLATMYLGGLGWFLVALRVKHSTHS
                     NRFNGSTMIIVMILLCVKSTDIGAYFGGRALGRRKLIPWLSPGKTWEGLFFGLLTAGI
                     VGAICARFISPPSYILPWWKGALFGIIIGGIGQAGDLLESLMKRDAEVKDSGRLVPGF
                     GGILDIIDSPLLAAPFAYLLFSLL"
BASE COUNT          330 a          542 c          604 g          276 t
ORIGIN      
        1 gcccagcgaa acgcccggtc cgcctgcgcg tgatgcacgg gctgatcgcc gtccaaatcc
       61 ggcgtgtcca caagaacggc gggacttgcc gtttcagcgg cgagcggcac gacgatcaac
      121 gcatcggcgc gcccgcgtgc cggcaggtct gacgcttgcg cgatcacgtg ctcgacgccg
      181 agccaacctt ccggcaccag cttcacatcg cgcacgatcg cgactgggcc cgaggtgaac
      241 gttcgccgga agctcgtcgc ggtgacggtg gcgccgacga tgcggttgac cagtgtgctt
      301 tttcccgtgc cggtcccgcc gaggatgaca gtcatgggga cggtgaggtt tgtcttgcgc
      361 agatggaagc gaagcgctgc cgcgtcggcg cgccagcgtt cgatcgccgt gcgcgccgcg
      421 gcgcccaatg gcggcgacat cgccgcgagg ccggcgtcca gccgttcgag cggggcggcg
      481 agtttttcag ggcgcatgac cggcatcgct ggcgtgaatc atacttgaag ttgcgatcaa
      541 agaaggctga acagcaggta tgcaaacggg gcggccaaga gggggctgtc gatgatgtcc
      601 agaatcccgc caaatcccgg caccagccgg ccgctgtcct tcacctccgc gtcgcgcttc
      661 atgaggcttt ccagcaggtc gcccgcttga ccgatcccgc cgatgatgat gccgaacagc
      721 gcgcccttcc accacggcag gatgtacgac ggcggactga tgaagcgggc gcagatcgcg
      781 ccaacgatgc ccgcggtcag taggccgaag aagaggcctt cccacgtttt gccggggctg
      841 agccagggga tcagcttgcg gcgcccgagc gcgcggccgc cgaagtacgc gccgatgtcg
      901 gtggatttca cgcagagcag gatcatcacg atgatcatcg tgctgccgtt gaatcgattg
      961 gaatgagtcg aatgcttgac gcgcagcgcg acgaggaacc agcccagccc gccgaggtac
     1021 atcgtcgcga gcacagtgcc ggccatgcga tggattgcgc cttgggtttc gcggtcccac
     1081 gcgcgcgtga gagcggcgag cagcatcacg aacacgatga tgaacgccag cgtcgatgcg
     1141 gcgatcggtt ggaaccaggt gaactgcgtg agaaacgcgt gcagcacgag caccgcggcg
     1201 ccgctggcgg cgaagcggcg atatggcgcg atgcgctcgg cggtgaacag cacggcgatc
     1261 tcgccgatcg ccagcggcag gatgatcacc aggatgacca gcaacccgag cccgccgacg
     1321 ccgccggggc ggacgcgctg cgtccattgc tgggcggccg agtcgagcca cagcagcagg
     1381 agcagactgc ccatcatcag cgggccgaag gtcaggcggt tcttgaggga ttgttccatg
     1441 cgtgaacgac gggggttgta ccggatgcgg cgtggaattg cattctgcgg aataatcggc
     1501 atcgattgtg cgcggtcata cgtagcacgg catctggcac atgccgaggt gccaggcacg
     1561 ggcgagacgc ccgtgctacg gaagagggca caccaatcac atcgaaggag ccactgcaat
     1621 gatcaagtct ggccaattgg gtttgctggg cgccaccctt ctcgccgtcg ccgtcctcga
     1681 aggctgtagc gggatgcaaa agggcgttgg catcgtgtcg tacgacaagg gcacgcagcc
     1741 gatcctggcc aa
//
LOCUS       DATIMH010000005         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5198878, whole genome shotgun sequence.
ACCESSION   DATIMH010000005 DATIMH010000000
VERSION     DATIMH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5198878"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            592..1215
                     /locus_tag="VLI90_00085"
     CDS             592..1215
                     /locus_tag="VLI90_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12625.1"
                     /translation="MFFSRRPWVQLTGAALLTAGYIKRGATHTFPRDGYTAAMNHIRL
                     AHVVLGVLGACSLILWIDASQDTPHGLVYQTNHPTNRLDEYGVIGYSNDLVVGHGRVE
                     TWQAGQPASIKGGVNGFVFDHVIDRPFALARGYDVVNFIKFDNSKTAGRFSWEETASG
                     FMTEQRLIIPAFLPLLLVLAATGIYVAYQYTRPADQPKGGFSVRLIR"
     gene            complement(1212..1583)
                     /locus_tag="VLI90_00090"
     CDS             complement(1212..1583)
                     /locus_tag="VLI90_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12626.1"
                     /translation="MHRCLFNLLAGVSLLLCVATAAAWADGHRHPSSTGYARPERRIH
                     HSRGSQRDQRRGERINSTHDEEIFDNTDTQPATTAFQDLAGTISYDEGDVQVYWGDLA
                     TGFNNRWDSTMFTNPLPVYQH"
     gene            1761..2204
                     /locus_tag="VLI90_00095"
     CDS             1761..2204
                     /locus_tag="VLI90_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12627.1"
                     /translation="MASTNIPPGKTLLAARIIWFALMMGELLFLGVTTFVILPNHGPA
                     KPQPQTILVWSSIFMAVAIIPVTFLIRSAIFRRTAVNGAVPAGPFVTGNIIFWAGCEG
                     VCFFATVVATVNGSLWPTIVVAVIALSLQALTFPVAERLYVPPDQ"
BASE COUNT          431 a          735 c          715 g          480 t
ORIGIN      
        1 gctcgcacat gtttatcccg acgcgtccgg tgcggcgcga gctaaagtgc atgatgcctg
       61 atatgacacc gcgcggggcg ccaccgacgt cgctgtcgac gaacgtgtag cggatcgggt
      121 ctccgcttgt cggcgcgttg catttaacgc accgaagcgg cagcgaaccg ccatcgggca
      181 caacgatcgt cgcgccactg cgaacggcat cactagcggg ttcctgaggt cgcataacta
      241 gtattcccgc gcgcaccgta ccatttgcgc ggtttctatt ttcgcgggac gaactccatt
      301 agatcatgtc gcctcgggac ttgtcaacaa aaacagaatc ttcttgctcc cgcgcaaacg
      361 cattatatgt tagggagaag gaagccatgt aggaccgggc ttcagcccgt cagttcacat
      421 cttctgcaca tgctcctcat ccagaatctc ctgccgcggt caggagcttg atgattctaa
      481 atctgcgggt cgaagagggt gacgaatgcc ggaatatcgt tccggttgag cgcggcgtat
      541 gcctggcgaa gcgcttcgat ctcagccgcc aaaggcggcg tcgtttggct catgtttttc
      601 tcccgccgcc cgtgggtgca actgacgggt gcggctttgt tgaccgctgg ctatataaag
      661 cgcggcgcga cgcatacatt tccccgcgat ggttatactg ccgccatgaa ccacatcaga
      721 ttggcgcacg tcgttctggg cgtactgggc gcttgctctc tgatcctgtg gatcgacgcg
      781 agccaggaca cgccccacgg cttggtctac cagaccaatc acccaacgaa tcgcctggac
      841 gaatacggcg tcataggtta cagcaacgat ctggtggtcg gccacggccg cgtcgagacc
      901 tggcaggccg gccaacccgc gagcattaaa ggcggcgtca acggattcgt tttcgaccat
      961 gtcatcgatc ggccgttcgc gctggcccgt ggctatgacg tggtcaactt catcaagttc
     1021 gacaactcca aaaccgcagg ccgcttctcg tgggaggaga cggcgagcgg attcatgaca
     1081 gagcagcggt tgatcattcc cgcctttctc ccgctcctgc tcgtgctggc ggccacaggc
     1141 atatacgtcg catatcagta cacgcggccg gcagaccagc cgaagggcgg gttttccgtc
     1201 cgcctgattc gctagtgctg atagaccggc agcgggttcg tgaacatagt gctgtcccag
     1261 cgattattaa acccagtagc aagatcaccc caatacacct gcacgtcgcc ttcatcgtag
     1321 ctgattgtac cggccagatc ctggaacgcg gtggtcgccg gctgggtgtc ggtgttgtca
     1381 aagatctcct catcatgcgt ggaattgatc ctctcgccgc gacgttggtc gcgctgtgat
     1441 ccccgtgaat ggtgaatgcg ccgctcagga cgtgcgtacc cagtcgaact tgggtgacgg
     1501 tgaccgtccg cccacgccgc tgccgtcgcc acgcacagca gcagcgacac cccggccagc
     1561 aggttgaaga ggcagcgatg cacgaccggt attttacggc cgtatcgagg cgccgaagat
     1621 cacctgcggt cggcggatcg agacgtgcga gccgctgcct gaggcgcgat cgtcggcggc
     1681 gcgatggttg gcgcgtcatc gcactcgacc gaaatcaatc gcgcttgtcg cgcccgcgcg
     1741 acgcggggac aatgcgcacg atggcctcaa ccaacattcc ccccggcaag acgctgctcg
     1801 ccgcccgcat catctggttc gcgctgatga tgggcgagct gctgttcctc ggcgtcacca
     1861 cgttcgtgat cctgccgaac cacggcccgg cgaaaccgca gccgcaaacg attctcgtct
     1921 ggtccagcat ctttatggcg gtggcgatca tcccggtgac gttcctgatt cgatccgcga
     1981 tatttcgtcg aaccgccgtg aacggcgctg tgcccgctgg tccgttcgtc accggcaaca
     2041 tcatcttctg ggccggctgc gaaggggtct gctttttcgc cacggtggtg gcgacggtaa
     2101 acggttcgct gtggccgacg atcgtcgtcg cggtgatcgc gctgtcgctg caggcgctga
     2161 cgttccccgt cgccgagcga ctgtacgttc cgccggatca atgaacggca acatcgggcg
     2221 cgcgggctcg cgcgggcgcg cgctggtgaa aaatgcgaac gcatccgcta tatactcgct
     2281 tctctgccgc cgcgcatgag cagacctgga gaggtggccg agcggctgaa ggcgcagcac
     2341 tggaactgct gttggcggct t
//
LOCUS       DATIMH010000006         1600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2888834, whole genome shotgun sequence.
ACCESSION   DATIMH010000006 DATIMH010000000
VERSION     DATIMH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2888834"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VLI90_00100"
     CDS             <1..1545
                     /locus_tag="VLI90_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSV12628.1"
                     /translation="SSVANNRPQLTTRIPDHGPTPMLFEPLFAHAATQPNDIAIIDDT
                     GRYTHQQLAGFAAGLGLYLSMQTSRDKVGLLLPAGAGFAASFYGTLLAGKSVVPINFL
                     LGEREITHVIADSGIDTVVTIPQLAGKLAGSNLKIVDLAALPKTPPAAITPRFPSPKA
                     DDLAVLMYTSGTSALPKGVMLTYGNLQTDVESAIAHAQLQGKHKFLGILPLFHSTGLL
                     ATLIAPITLGAQTVYIARFSPVATLNAIREHQISIVAAVPAMYAAIVRLKNAAPEDFQ
                     PLYAAISGGEPLPAAVREAFQARFGQPIYEGYGLTETIGPIAFNVPEHIRPGSVGRLI
                     PGASAKIVDDDGKEVAQGQPGEIWLKGPMVMKGYYNLPNETAAALTADGFFKSGDIGF
                     LDPDGYLHVTGRKKELIIVAGEKAVPREIEDVLLRHPGVADAAVVGKKDPSRGEVVVA
                     FVIPKEGQTVKPDELRDFARQQGLVQWKIPREVTVVQDLPRSPTGKVLKRVLSEQVNA
                     AAGKSQ"
BASE COUNT          313 a          522 c          509 g          256 t
ORIGIN      
        1 tcgtccgttg cgaacaaccg cccacaactg acgacgcgga tccctgacca cggacccact
       61 ccaatgcttt tcgaacccct cttcgcccac gccgccacgc agcccaacga catcgcgatc
      121 atcgatgaca ccggccgata cacgcaccag cagttggcgg gattcgccgc ggggctgggg
      181 ttgtatctgt cgatgcagac cagccgggac aaggtcggcc tgctgctgcc ggccggcgcg
      241 ggcttcgccg ccagtttcta cggaaccctg ttggctggca agagcgtggt gccgatcaac
      301 ttcctgctcg gcgagcgcga gatcacgcat gtcattgctg atagcggtat cgatacggtg
      361 gtgacgatcc cgcagctggc gggaaagctg gcggggtcga acctgaagat cgtcgatctt
      421 gcggcgctac cgaagacgcc gcccgcggcg atcacgccgc gctttccatc acccaaggcc
      481 gacgacctgg ccgtgctgat gtacaccagc ggcaccagcg cgctgcccaa gggtgtgatg
      541 ctgacctacg gcaacctgca gacagacgtc gagtcggcca tcgcgcacgc gcagctgcag
      601 ggcaagcaca aattcctggg catcctgccg ctgttccatt cgacggggtt gctggcgacg
      661 ttgatcgcgc cgatcaccct gggtgcacaa accgtctaca tcgcgcgctt cagccccgtg
      721 gctacgctga acgccattcg cgagcaccag atttccatcg tcgccgccgt cccggcaatg
      781 tacgcggcga tcgtccggtt gaagaacgcc gcccccgaag attttcaacc gctgtacgcc
      841 gccatcagcg gcggtgaacc gctgcccgcc gcggtgcgcg aggcgtttca ggcccgcttc
      901 gggcagccga tctacgaagg ctacggcttg accgagacga tcgggccgat cgcgttcaac
      961 gtgccggagc atattcgtcc cggctcggtc gggcgactga tccccggcgc gagcgcgaag
     1021 atcgtcgatg acgacggcaa ggaagtcgcg cagggtcagc ccggcgaaat ctggctcaag
     1081 gggccgatgg tcatgaaggg ctactacaac ttgcccaacg aaaccgccgc cgcgctgacc
     1141 gctgatggct tcttcaaatc cggcgacatc ggtttcctcg accccgacgg atatctgcac
     1201 gtcaccggcc gcaagaagga actgatcatc gtcgccggtg aaaaggcggt gccgcgcgag
     1261 atcgaggacg tgctgctgcg ccaccccggc gtcgccgacg cagcggtggt aggcaaaaag
     1321 gaccccagcc gcggcgaggt cgtcgtcgct tttgtgattc caaaggaagg ccagacggtg
     1381 aagccggacg agctgcgcga tttcgcgcgg cagcaggggc tggtgcagtg gaagatcccg
     1441 cgcgaggtga cggtggtgca ggacctaccg cgatcgccca cggggaaggt gctcaagcgc
     1501 gtgctgtccg agcaggtgaa tgcggctgcg ggaaagtccc aatgacgaag cccgaattcc
     1561 cgaatgaaac ccgaatgaaa cccgaatgac gaagcacgaa
//
LOCUS       DATIMH010000007         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1445299, whole genome shotgun sequence.
ACCESSION   DATIMH010000007 DATIMH010000000
VERSION     DATIMH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445299"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VLI90_00105"
     CDS             complement(<1..1394)
                     /locus_tag="VLI90_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HSV12629.1"
                     /translation="MPGMTMTEKILAKHSGKTRVQPGENVWVNVDVLMTHDVCGPGTI
                     GIFHDKFGKNAKVWDKDRVVIIPDHYIFTADQKCHRNVQTLRDFVKEQGLRYYYDPEF
                     VTMEPTMPNPYRDPTKTNYKGVCHKALPEEGHIRPGEILLGTDSHTCTAGAFGQFATG
                     VGNTDAAFVLGTGKTWLKVPPTMRFTFNGEIPPYLTAKDLILAVIGEIGVDGATYRAM
                     YFAGDGIGSLTLEDRMTLTNMAIEAGGKNGICDVDEKTLQYVRARSNRPDWEVVTEDG
                     DAQYHFEASWDLATMEPMVAKPHSPDNRDTAHNCRDVKLDQAYIGSCTGGKITDMIFA
                     ANLLKGHTVKIPTFVVPGSTEVHSDMKRLNLRGVEKDTNEKSIEDVLQDAGCNVGASG
                     CAACLGGPSDTFGRLQRSEVCISTTNRNFPGRMGSTKSAVYLASPLTVAASALTGHVT
                     DPREYVSTKIETGMA"
     gene            complement(1475..1768)
                     /locus_tag="VLI90_00110"
     CDS             complement(1475..1768)
                     /locus_tag="VLI90_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSV12630.1"
                     /translation="MAFKIVWSPEAVQQLHEISEYIARDSTTYAAGVILHIIEAAERL
                     ADHPRLGRRVPEWDRDELREVLSGNYRIIYRVGNGRVDITDVMHGAQQLRQEP"
     gene            complement(1753..1962)
                     /locus_tag="VLI90_00115"
     CDS             complement(1753..1962)
                     /locus_tag="VLI90_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12631.1"
                     /translation="MSVAQSQLPPVKEQVRALLEHLPDDCTVEDVQYRLYVLERIRRG
                     LESIDRGEGIPHEEVKRRMAQWLSK"
     gene            2039..2812
                     /locus_tag="VLI90_00120"
     CDS             2039..2812
                     /locus_tag="VLI90_00120"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSV12632.1"
                     /translation="MGIYDRDYTKVNYAPRGRAPAFGSVRMWSITTWLIIINVAVYLL
                     DRLLDNATGGYNFYIIVGDYRIPQHFAPLEYWGHFSAFTAIAHLQLWRFITFQFLHAS
                     PSHLIYNMLALFFFGPIMESYLGSRRYLLFYLLCGIAGAFSYLVLWGMHLLVTESWQP
                     LVGASAGIFGILIAAAQVAPDTTVLIYGIVPMRLRMMAWILLGIAVYTVLTMGHNAGG
                     EAAHLGGAILGVVLIRNPRVLRFADSIGASRSRMRYRGR"
     gene            3019..3468
                     /locus_tag="VLI90_00125"
     CDS             3019..3468
                     /locus_tag="VLI90_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HSV12633.1"
                     /translation="MHKTHIPRRWRTGRAGSAILEMSLIAPLLFMLSFGVADYGYFLY
                     VKNTFQGAAQAGARAAVPYAATNANVTGTTGIVTKMMTAAGVPSANYTVTLSPSNISG
                     ISAGTLITVTLSANWSTVGTHILPASMGGLGSSKVVSATAIAQKESN"
BASE COUNT          609 a         1177 c         1072 g          772 t
ORIGIN      
        1 gccatgccgg tttcgatctt cgtcgaaaca tactcgcgcg gatccgtcac atgccccgtc
       61 agcgcgctgg cggcgacggt cagggggctc gccagataca ccgcactctt cgtgctgccc
      121 atgcggccgg ggaagttgcg gttcgtcgtg ctgatgcaca cttcactgcg ttgcaggcgg
      181 ccgaaggtgt cgctgggacc gcccaggcag gcggcgcagc cggatgcgcc gacgttgcag
      241 ccggcgtctt gcagcacgtc ttcgatgctc ttttcgttcg tgtccttctc gacgccgcgg
      301 aggttcagcc gcttcatgtc cgagtgaact tcggtcgatc ccggaacgac gaacgtcggg
      361 atcttcaccg tgtgaccctt gagcaagttg gcggcgaaga tcatgtcggt gatctttccg
      421 ccggtgcacg agccgatgta cgcctggtcc agcttcacgt cgcggcagtt gtgggcggtg
      481 tcgcgattgt cggggctgtg cggcttggcc accatcggtt ccatcgtcgc caaatcccac
      541 gacgcctcga agtgatactg cgcgtcgccg tcttcggtga cgacttccca atcgggccga
      601 ttgctgcggg cgcggacgta ctgcagcgtc ttttcatcga cgtcgcagat gccgttcttc
      661 ccgccggctt cgatcgccat gttcgtcagc gtcatgcggt cttccagcgt gaggctgccg
      721 atgccgtcgc cggcgaaata catcgcgcga tacgtcgcgc cgtcgacgcc gatctcaccg
      781 atgaccgcca ggatcaaatc cttcgccgtc aggtacggcg ggatttcacc attgaaggtg
      841 aagcgcatcg tcggcgggac cttcagccac gttttgccgg tgcccagcac gaaggccgcg
      901 tcggtgttac ccacgccggt ggcgaattgg ccgaacgcgc cagcggtgca cgtgtggctg
      961 tcggtgccca gcaaaatctc gccggggcgg atatgccctt cttccggcag cgccttgtgg
     1021 cagacgccct tgtagttggt tttcgtagga tcgcgatacg gattcggcat cgtcggttcc
     1081 atcgtgacga attccggatc gtagtaatat cgcagcccct gctccttcac gaagtcgcgc
     1141 agcgtctgca cgttgcgatg gcacttctgg tcggcggtga agatgtagtg gtccgggatg
     1201 atgaccacgc ggtccttgtc ccacaccttg gcgttcttgc cgaacttatc gtggaagatg
     1261 ccaatggtcc ccggcccgca gacatcgtgc gtcatgagca cgtccacgtt cacccaaacg
     1321 ttttcgccgg gctgcacgcg cgttttaccg ctgtgcttgg ccagaatctt ctccgtcatc
     1381 gtcatgccgg gcattcgcaa actcctgtag ctggtcgcgg aacgaaaaga gagctacgag
     1441 tatagccgca aggcggttcc ggttctatcg cggtctatgg ctcttggcga agctgctgcg
     1501 cgccatgcat cacatcggta atatccacgc gaccatttcc cacgcggtag atgatccgat
     1561 agttgccaga aagcacttcc cgcagttcat cccgatccca ttcaggcact cgcctgccca
     1621 agcgaggatg atcagcaagc ctttccgcag cctcgatgat gtgcaagata acgccggccg
     1681 cataagtggt ggaatcgcgc gcaatgtatt cagagatctc gtgcagttgc tgcacggctt
     1741 ccggggacca gactattttg aaagccattg ggccatccgg cgtttgactt cctcgtgcgg
     1801 aatgccctcg ccacgatcaa ttgattccag cccgcgccga atccgctcta gaacgtaaag
     1861 ccgatactgc acatcttcca cggtgcaatc atcgggcaga tgttcaagta gcgcacggac
     1921 ctgctccttc accggcggta gttgtgattg ggcgacgctc atagccacat tatatcacgc
     1981 cccccccgca gagcacgttg tcctttcatt cctctcgccc ctacacttgg cgcggcacat
     2041 gggcatctac gaccgcgatt acacgaaggt gaactacgca ccgcggggcc gcgcgccggc
     2101 cttcggctcg gtccgcatgt ggtcgatcac cacctggctc atcatcatca acgtcgcggt
     2161 ctacctgctc gatcgcctgc tcgacaacgc caccggcgga tacaactttt acatcatcgt
     2221 cggcgattat cgaatccccc agcacttcgc gccgctggaa tactggggac acttttccgc
     2281 cttcaccgcc atcgcgcacc tgcagctgtg gcggttcatc acgttccagt tcctgcacgc
     2341 cagcccgtct cacctgatct acaacatgct cgcgctcttt ttcttcggcc cgatcatgga
     2401 aagctatctc ggctcacggc gatacctgct gttctacctg ctctgcggca tcgccggggc
     2461 gttcagctat ctggtcctgt ggggcatgca cctgctggtg accgagtcgt ggcaaccgct
     2521 ggtcggcgcg tcggccggca tcttcggaat cctgatcgcc gccgcccagg tcgcgccgga
     2581 tacaacggtg ttgatctacg gcatcgtgcc gatgcggctt cgcatgatgg cgtggatcct
     2641 gctcggcatc gcggtgtaca ccgtgctgac gatgggccac aacgccggcg gcgaagcggc
     2701 gcacctcggc ggcgcgatcc tcggggtggt attgatccga aatccgcggg tgctgcggtt
     2761 cgcggactca atcggcgcca gccgatcgcg catgcgctat cgtggccggt gatcggctct
     2821 cgcccgtgat cgttatccct tgcagtattc agatgcagcg atccagccca gccgtgagtt
     2881 attggactgc gcaccaaccg gcgatctcgc cacctcgcgc ggccgactct aacgcggttc
     2941 tcatgtgccc caacacatac gccgatcaac atgtggatgc ttatcgggga gcctcacctg
     3001 tcgcggcgac aattgttcat gcataagacc cacatccccc gccggtggcg aaccgggcgc
     3061 gcgggcagcg cgatcctcga gatgtcgctg atcgccccgc tgctgttcat gctgtcgttc
     3121 ggcgtggcag attacgggta cttcctctat gtgaagaaca cgttccaggg cgccgcccag
     3181 gccggcgcgc gggcggcggt gccctacgcg gcgaccaacg ccaacgtcac cggcacgacc
     3241 ggcatcgtca ccaagatgat gaccgccgcc ggggttccca gcgcgaatta cacggtgacg
     3301 ctgagcccgt cgaacatcag cggcatctcg gccggcacgt tgatcaccgt gacgctttcc
     3361 gcgaactggt ccactgtcgg cacgcacatc ctgccggcgt cgatgggtgg gttgggcagc
     3421 agcaaggtcg tctccgctac cgcgatcgct cagaaggaat cgaactgatt atggctcgtt
     3481 cgtctcgaca tcatcgtcgt cgtggagcgg tctttcttta tctgctgctc ggcatcgtga
     3541 cgttcaccgc gctggtgtcg ctgggggcgg acgtggcgca tgtgcgcgtc gtgaagaacc
     3601 aactgcagtg cgccgccgac ctggccgccc
//
LOCUS       DATIMH010000008         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5199443, whole genome shotgun sequence.
ACCESSION   DATIMH010000008 DATIMH010000000
VERSION     DATIMH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5199443"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..1296)
                     /locus_tag="VLI90_00130"
     CDS             complement(286..1296)
                     /locus_tag="VLI90_00130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV12634.1"
                     /translation="MQEPPNVKRVLVISIDPQEASFRLRMAILPRLLAADHYEFEFHT
                     RPKKWLDRLKLVASASRYHAVIVQRKLLDPSHAKILRWRSRRVVYDLDDAVMVQRRKI
                     GRMSQWLKQRRFIATARSADHVVAGNQFLADTFVKLDCPVTVLPTVVDPQHYKIKSHA
                     ATDAPTLVWIGSRSTLPYLQQWMTAIEAATRRVPGLRLTTIANDTVRSEVIPVEHVAW
                     SVEAESAALHRGDIGIAPTPSDQWTIGKSGFKIIQYMATGLPTVASPVGANEQILIDG
                     TTGLLASSMEQWTDAIVRLANDVSLRAQMGAAARRLVETDYSLERARHVWREVLEGER
                     SS"
     gene            1481..2362
                     /locus_tag="VLI90_00135"
     CDS             1481..2362
                     /locus_tag="VLI90_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HSV12635.1"
                     /translation="MPVPSARQPVIILGMHRSGTSMVSQLLDQLGLFVGRELQDDHES
                     TYFLSINETIFARVGASWDNPLPMRYFLANPDAVQMTAGAVAADLSSRRIGTFFSPRG
                     IFSRQSLKNFDRPWGWKDPRTIFTLPVWLQLFPDARLIYIIRNGVDVASSLMVREQRL
                     LERRKSRFEARLAAKLSHRSTLERAGYKGSPRCFTLDGGFDMWREYVEEADRQLKGLS
                     NPILSMRYEDILEDPQKHLTEMAKFNQLDGATPAAVEQAAAKVNKSRARAFSGDRVLA
                     GFYEKIRNDPWMVRHGY"
     gene            2478..3620
                     /locus_tag="VLI90_00140"
     CDS             2478..3620
                     /locus_tag="VLI90_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HSV12636.1"
                     /translation="MSDTRINPLLATHQQAQAELQAYGEIEIVSTFGEPQAEYAAIRK
                     GAAMIDLPQRGILELTGKDRLPFLNNLLTNQTYDKNTKTGVSAGRGVYAFLLNAKSGR
                     IIADTNVLELGERTLLEMDARLVLTVAEALEKYRFSEQVKFSDQVGQFHEIALHGPQA
                     LAVLSEALGSPMPAMEPLGVWQTTLFGVPTIIWRDDPAAVPGYHLIVPTEAARTIWMN
                     LLSRFGASTESGKRSLRPTGWAAFNATRIESGRPLFGIDFDDTILPAETGQLSRAVSF
                     TKGCYPGQEIVARMHARQQVAKQLVGIRFADDHLPMAGATVFDDQSNQIGGITSSTIS
                     PVLSNAAIALGYVKKPFIPVGSVVSVPAEGALRRGIVAALPFVERV"
BASE COUNT          731 a         1218 c         1138 g          674 t
ORIGIN      
        1 taaaaacacg cgatgccggc gaacagccac catcgatgcc ggtccgccca cgcgagcagc
       61 caatcgcaga acgcgacgcg ctgccacagc ggcagctccg ccgcgtcaag cgaacgatcc
      121 gcgaagttgc caccgatggc tgattccaaa aacgctgacc tttaaaagat cccatttagc
      181 ccggcgtgaa cacatgccgg gttacgctga gaagtcgcgc gtgcctaatt tctcaatcag
      241 aggtccgcga agacccacat agacccggcg tgtgttcacg ctggcctaac tggaacgttc
      301 gccttccagc acttcccgcc acacgtgccg cgcgcgctct aaactgtagt ccgtctcaac
      361 caatcgtcgc gccgccgcgc ccatttgcgc tcgcagcgac acatcgttag ccaagcgcac
      421 gatcgcgtcg gtccattgct ccatagacga cgccagcagg cccgtcgttc catcgatcag
      481 gatctgctcg ttcgcgccca cgggcgacgc aacggtcggc aatcccgtcg ccatgtactg
      541 gatgattttg aatccggact tgccgatcgt ccactggtca ctcggcgtcg gggcgatccc
      601 gatgtcgccg cgatgaaggg cggcggactc agcttccacc gaccacgcga cgtgctccac
      661 cgggattacc tcgctgcgca ccgtgtcgtt cgcgatcgta gtcaaccgca accccggcac
      721 ccgccgggtg gcggcctcga tcgccgtcat ccattgctga aggtacggca acgtggaccg
      781 cgagccaatc cacaccagcg tcggcgcgtc cgtcgccgcg tgcgatttga ttttgtaatg
      841 ctgcggatcg acgaccgtcg gcagcaccgt caccggacaa tcgagcttca cgaacgtgtc
      901 ggcaagaaac tgattgcccg cgacgacgtg atcggcgctc cgggcggtcg cgatgaatcg
      961 ccgctgcttc aaccactgcg acatccggcc gatcttccgc cgctgcacca tcacggcatc
     1021 gtccaggtcg tatacgaccc ggcgcgaccg ccagcggagg atttttgcgt gcgacgggtc
     1081 cagcaacttt cgctgcacga tcacggcgtg atatcgactt gccgatgcga cgagcttcaa
     1141 acggtccagc cacttctttg gacgcgtgtg gaattcgaat tcgtaatgat cggccgcgag
     1201 cagccgcggc aggatcgcca tgcgcaggcg gaaggacgcc tcttgcggat cgatgctgat
     1261 caccagcacg cgcttgacgt ttggcggctc ctgcaatgcg ggggtttcct gctcgacaaa
     1321 caaggcccac ggctctgccg tgggacgtct cttaccagtc cgaacccacg gcagagccgt
     1381 gggcttcttc actttttctc gatcacgttg ccgctgcacc gggcgtgcgg tagcatcgcg
     1441 cgcccggcct gcggctcgtc gtgtcgcgcg ccggcagctt atgccagttc ccagcgcgcg
     1501 acaacccgtc atcatcctgg gcatgcaccg ctccggcacg tcaatggtgt cgcagttgct
     1561 cgatcagctc ggcctgttcg tcggccgcga attgcaggac gatcacgagt ccacttactt
     1621 cctttcgatc aacgagacga ttttcgcccg cgttggcgca tcgtgggaca acccgctgcc
     1681 gatgcggtat ttcctggcca atcccgacgc ggtgcaaatg accgccgggg ccgtggcggc
     1741 ggacctgtcg tcgcgccgca tcggcacatt cttcagcccg cgcgggattt tttcgcggca
     1801 gtcgctgaag aattttgatc ggccgtgggg atggaaagac ccgcgaacga tcttcacgct
     1861 cccggtctgg ctgcagctgt ttccggacgc gcggctgatc tacatcatcc gcaacggcgt
     1921 tgacgtcgcc agcagtttga tggtccgcga gcagcgcctg ctcgaacgtc gaaagagccg
     1981 gttcgaagcg cgcctcgcgg cgaagttgag ccatcgctcg acgttggaac gcgcgggata
     2041 caaaggttcg ccccgatgct tcacgctcga tggcgggttc gacatgtggc gcgaatatgt
     2101 cgaagaggcg gatcggcagc tcaagggact aagcaatccg atcctgtcta tgcgctacga
     2161 ggacatttta gaagacccgc aaaagcacct gacggagatg gcgaagttca accagctcga
     2221 cggtgccacg ccggccgcgg tcgaacaggc cgcggcaaag gtgaacaaat cgcgggcacg
     2281 cgcgttctcc ggcgatcgag ttctcgcggg cttctacgag aagatccgga acgatccgtg
     2341 gatggtccgg cacggatact aaaatttgat ccatctgccc gcgtagcggt agccacgcag
     2401 gggcacggtg ttttccgttc gagcgcgcag aaacaagtgc ccctgtgggg caaccgctac
     2461 gcggcataag atgacatatg tctgacacgc gaatcaaccc gttgctcgcg acgcatcagc
     2521 aggcgcaggc cgagctgcag gcgtatggcg agatcgaaat cgtcagcacc tttggcgagc
     2581 cgcaggcgga gtacgctgcg attcgcaagg gcgccgcgat gatcgacctg ccgcaacgcg
     2641 gcatccttga gctaaccggc aaggaccggc tgccgttcct caacaatctg ctgaccaacc
     2701 agacgtacga caagaacacc aagactggcg tgtccgccgg ccgcggcgtg tatgcgtttc
     2761 tgctgaacgc caagtctggc cggatcatcg cggatacgaa cgtgctggag ctgggcgagc
     2821 gcacgctgct ggagatggac gcccggctcg tcctcaccgt cgccgaggcc ctcgaaaagt
     2881 atcggttcag cgagcaggtg aaattcagcg accaagtcgg ccagtttcac gagatcgcgc
     2941 tgcacggtcc gcaggcgctg gcagtactta gcgaagcgct cggttccccg atgccggcga
     3001 tggagccgct tggcgtttgg caaaccactt tgttcggtgt ccccacgatc atctggcgcg
     3061 acgaccccgc cgccgtcccg gggtatcacc tgatcgtccc gaccgaagca gcgcgcacga
     3121 tctggatgaa cctcttgtcg cgtttcggcg cttcgactga aagcggcaag cgttcgctgc
     3181 gccccaccgg ttgggcggcg ttcaatgcca cgcgcatcga aagcggccga ccgctgttcg
     3241 gcatcgactt cgacgacaca atcctgcccg ccgaaaccgg acaactgtcg cgggcggtca
     3301 gcttcaccaa ggggtgttac ccgggccagg aaatcgtcgc ccgcatgcac gcccgccagc
     3361 aggtggccaa gcaactggtc ggcatccgct tcgccgacga tcacctgccg atggccggcg
     3421 ccacggtctt cgacgatcaa tccaaccaga tcggcggcat caccagtagc acgatatcac
     3481 cggtgctgtc gaacgcggcc atcgccctgg gctacgtgaa gaaacccttc atccccgtgg
     3541 gcagtgtggt aagcgtccca gccgaaggcg cccttcgccg cggcatcgtc gcagcgttgc
     3601 cgttcgtgga gagggtttga accaccaaga gcacaaagcg cacgaagcgg agaaataggt
     3661 tttagccaca gatgaacgcc gatggacgca gatgaagagt aagagggagc gaagggctct
     3721 tgagtttgag gagtgaacgc acgggccgga ggcccgtgtc a
//
LOCUS       DATIMH010000009         4484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3755624, whole genome shotgun sequence.
ACCESSION   DATIMH010000009 DATIMH010000000
VERSION     DATIMH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3755624"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VLI90_00145"
     CDS             <1..998
                     /locus_tag="VLI90_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12637.1"
                     /translation="APDVNLAAFREALRREDVATSADGNANRVSDVIARLADQVVAPL
                     REARREVDRLTASLRAMEAMPAGVDVNPITQQLQRRLQQRSVLYLIGPQRVLDRVRQV
                     PTMLARLPRATWDWVMRGELPSDIDPKSNGRQGEPPDFRAALNDQFTVVRSRIDDILR
                     SNPLAERWLNDNAASYNQSLLDPANAGKIADEEIENLKQWLETRWNATPRDTRVLQAL
                     LKYLPGGRQLTRWSEAAPYLLTIAVVTHHAFFGHIDLMVLGGYSLATWLTERISNEVA
                     SRTRATNRKIAERFERLAHEQIDRVCAWLDQQAPSERALDRVEHMADELENVTRD"
     gene            1134..2837
                     /locus_tag="VLI90_00150"
     CDS             1134..2837
                     /locus_tag="VLI90_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12638.1"
                     /translation="MTPELQQLVHETSELTGAAAPDLLGHDAPTLVSDALKDQDFYLV
                     GLIGGKEVGKSALINALVGEPITQTTSFGPGTETVVAYAHESQADAIRQLLDREVPGQ
                     FRLVTHRLSHLSRQVLLDLPDIDSHWQAHVEITRRMLRHMLFPLWVQSVEKYADHQPQ
                     QLLAKVAQGNAAVNFVFCLNKVDQLGASNGDIEELRADHAKRISRLLHIDPPPRVWML
                     SAVRPDQYDLPGLRAMLAQQKSTDLVRQSQSLASRRQNQLIVAWLDAQDLAGRADRLA
                     RLQQSAEELVATRIGVPLLEQSLPALADDPASRLALTDEVLAARVARWPIVNLIQTVL
                     TPVLAIVRRNVGATRTASLPDAEALVEAHLQLRGKPLAALVGSTFAQLQQSNPSISEL
                     YRARKLWDAMTADATAAQLRAGLTDTVARQRDAVRQRLLGKGGLMSAIFAPIRWLLTI
                     GALLWFPFLQPITEKFLTEDLRHSAHDIILMAVRIFSVNELLANLTFLGMWYFVLWVI
                     LRWDTQRRVARLANRWKSDSSDLSLAAQTLRWLDDLLAPIRSMREQADALRQRVEAIR
                     Q"
     gene            2905..3384
                     /locus_tag="VLI90_00155"
     CDS             2905..3384
                     /locus_tag="VLI90_00155"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HSV12639.1"
                     /translation="MESPTLSQPTARPIRVVPPVYLLAAVILMLLLHRFAPVRQLIQW
                     PWRWIGVAVIVGGLAFGFSAVALFRRHRTPLRPGQASSSLVDSGPFRVTRNPIYVGMT
                     IALAGVAVLLGSLTPWAVVPLFVLVINRNVIPLEERMLLGAFGEEYTRYQARVRRWI"
     gene            complement(3469..3639)
                     /locus_tag="VLI90_00160"
     CDS             complement(3469..3639)
                     /locus_tag="VLI90_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12640.1"
                     /translation="MKSIKNRLMCLLSDEQGGEVLEYALIAGLIVVAAIAVIASVGDK
                     VLARWGSLNSSI"
     gene            complement(3802..>4484)
                     /locus_tag="VLI90_00165"
     CDS             complement(3802..>4484)
                     /locus_tag="VLI90_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV12641.1"
                     /translation="NGSLSPTTTVNDSGSLSFGSNAGTGSLARTIGALNISANVTANL
                     VASAFPAAPAVLNAGTLSFADTSAKLDLGNNELLTNATAPDIRALIVAHQIFTSSTGG
                     VIGYADQGGGVTEVRFTLAGDANLDGSVDVADLGRLATNYNSAMPEWTAGDFDYNGTI
                     DVGDLGALATNYGTSLASGPSAQSIATPTASTAIEQSTVPEPASAGVLALGAAACLAG
                     RQRRRSRR"
BASE COUNT          796 a         1446 c         1447 g          795 t
ORIGIN      
        1 cggcgccaga cgtcaatctc gccgcgtttc gcgaggcgct gcgtcgcgaa gacgttgcga
       61 catccgccga tggcaacgcc aatcgcgtca gcgatgtcat cgcgcgactg gccgatcaag
      121 tagtcgcccc gctgcgcgaa gcgcggcggg aagtcgatcg attaaccgca tcgctgcgcg
      181 cgatggaagc gatgcccgcg ggcgttgacg tcaatccgat cacgcagcaa ctgcagcggc
      241 gactgcagca gcgcagcgtg ctgtacctga tcggcccgca gcgcgtgctc gaccgcgtgc
      301 ggcaggtgcc gacgatgctc gcccgcctgc cgcgcgccac ctgggactgg gtcatgcgcg
      361 gtgaattgcc cagcgacatc gaccccaaat ccaacggccg gcaaggcgaa ccgccggact
      421 ttcgcgcggc gctaaacgac caattcaccg tcgtccgcag ccgcatcgac gacattctgc
      481 gatcgaatcc gctggcggag cgctggttga atgacaatgc tgcaagttac aatcaatccc
      541 tgctggatcc ggccaatgcg ggcaagattg ccgatgagga aatcgagaac ctgaagcaat
      601 ggctggagac gcgttggaac gccacgccgc gcgacacgcg cgtgctgcag gcattgctaa
      661 agtacctgcc gggcggccgg cagctgacgc ggtggtcgga ggcggcgccg tacctgctga
      721 cgatcgccgt cgtcacgcac cacgcgttct tcggtcacat cgacctgatg gtcctcggcg
      781 gctatagcct ggccacgtgg ttgaccgaac gcatcagcaa cgaggtcgcc tcgcgcaccc
      841 gcgcgacgaa ccgaaagatc gccgagcgct ttgaacggct ggcccacgaa cagatcgacc
      901 gcgtgtgcgc atggctggat caacaagcgc catcggagcg ggcgctggat cgcgtggaac
      961 acatggcgga tgaattggag aatgtgacgc gggattaatt ctggttccct cgcccatgta
     1021 ctcatgggag agggttaggg tgagggtgat ttggaaggcc gaaagccact ggtgctcgaa
     1081 atcaccctca ccccggccct ctcccacggg tatctgggcg agggggaaat acgatgacgc
     1141 cggaactgca acaactcgtt cacgaaacat cggagctgac cggtgcggcg gcgccggatc
     1201 ttctgggaca cgatgcgccc accctcgtga gcgacgccct gaaggaccaa gatttttacc
     1261 tcgtcggcct gatcggcggg aaagaggtcg gcaagagcgc gctgatcaac gcactggttg
     1321 gcgagccgat cacacagacg acatccttcg gccccggcac ggaaaccgtc gtcgcatacg
     1381 cgcacgaatc gcaggcggac gcgatccgcc aactgctcga ccgcgaggtg ccggggcagt
     1441 ttcgcctcgt cacgcatcgc ctctcgcacc tgtcgcgaca agtgctgctg gacctgccgg
     1501 acatcgacag ccattggcaa gcgcacgtcg aaatcacccg ccggatgttg cggcacatgc
     1561 tgttcccgct gtgggtgcag agcgtggaga aatacgccga ccaccagccg cagcagctgc
     1621 tggcgaaggt tgcccaaggc aacgccgcgg tgaacttcgt gttttgcctg aacaaggtcg
     1681 atcaactcgg cgcgagcaat ggcgacatcg aagagctgcg cgcggaccat gcgaagcgga
     1741 tctcccggct gctgcacatc gatccgccgc cgcgagtgtg gatgctcagc gctgtccggc
     1801 ctgaccagta cgatctgccg ggcctgcgcg caatgctcgc tcaacaaaaa tcgacggacc
     1861 tagttcggca gtcgcaatcg ctggccagcc gccggcagaa tcaattgatc gtcgcgtggc
     1921 tagacgccca ggatctggct ggccgagccg atcgcctcgc gcgcctgcag cagtcggcgg
     1981 aggaactggt cgcaacgcgc atcggcgtcc cgctgctgga acaatcgctg ccggcgctgg
     2041 cggacgaccc ggcatctcgc ctggcgctga cggacgaagt cctcgccgcc cgcgtcgcgc
     2101 gatggccgat cgttaacctg atccaaaccg tgctcacgcc tgtcctggcg atcgtccggc
     2161 gcaacgtcgg cgcgacgcgc accgcgtcgc tgccggatgc cgaagcgctg gtcgaagcgc
     2221 acctgcagct gcgcggcaag ccgctggcgg cgctggtcgg ctcaaccttc gcgcagctgc
     2281 agcagtcgaa cccttcaatc agcgagttgt atcgcgcccg aaagctgtgg gacgccatga
     2341 ccgccgacgc gacggcggcg cagctgcgcg ccgggctgac ggacacggtc gcgcggcagc
     2401 gcgacgccgt gcgccaacgc ctgctgggta agggtggact tatgagcgcc attttcgcgc
     2461 cgatccgctg gctgctgacg atcggcgcgc tgctgtggtt cccgttcctg cagccgatca
     2521 ccgaaaagtt tctgaccgaa gacctgcggc actcggcgca cgacatcatc ctgatggccg
     2581 tccgcatctt cagcgtgaac gagttgctcg cgaacctgac attcctgggc atgtggtatt
     2641 tcgtgctgtg ggtgatcctc cgttgggaca cgcaacgccg cgtcgcgcgg ctggcgaatc
     2701 gctggaagag cgactcgtct gatttgagtt tagccgccca gacgctgcgc tggctggacg
     2761 atctgctggc gccgatccgc tcgatgcgcg agcaagccga cgccttgcgc cagcgtgttg
     2821 aagccatccg ccaataatcg aatggcgcta cgcttgcgaa gatatgcgta gaccctgtcg
     2881 agggggcgcc tatgatctcg ccgcatggaa tcacccacgc tatcgcagcc gaccgcccgc
     2941 ccgatccggg tggtcccgcc ggtgtatctt ctggcggcgg tcatcctaat gcttctgctt
     3001 catcgatttg ccccggtgcg gcaattgatt cagtggccgt ggcgctggat cggagtcgcc
     3061 gtcatcgtcg gtggactggc cttcggcttc tccgccgtcg cgctgtttcg gcggcatcgc
     3121 acccccctgc ggccggggca ggcatcgagt tcgctcgtcg atagcgggcc gtttcgcgtc
     3181 acgcgaaatc cgatttacgt cgggatgacg atcgcgctcg ccggcgtggc ggtgctgctt
     3241 ggcagcctga cgccgtgggc ggtggtgccg ctgtttgtct tggtgatcaa ccgcaacgtg
     3301 atcccgctgg aagagcggat gttgctcggc gcgttcggcg aggaatacac gcgatatcag
     3361 gcgcgcgtgc ggcgttggat ttgattttcg tccggcgaca accgcgaccc aaaaacgtca
     3421 cgccgggttc ggacatgaat ccgaacccgg cgcggtcgcc tctgtgaatc agatcgagct
     3481 gttgagcgat ccccagcgtg ccagcacttt gtcgcccacg gacgcgatga cggcgatggc
     3541 cgccaccacg atcagcccgg cgatcagcgc gtactcgagc acttcgccac cttgttcatc
     3601 cgacaacaaa cacatcaagc gatttttgat gctcttcacg attctgctcc attcccttgg
     3661 cgattgacga actacacgcc agccgtatcg gccgcacgat tcgcgggctt cagatttgtg
     3721 gcgagattgc agaaagcgcg tagcggaaat tcggcagatg tgcgtgcgca cgagccggga
     3781 tatgcgtgtt atcggacgcg atcagcgccg gctgcggcgg cgttggcgtc cagctaagca
     3841 tgcggcggct ccgagcgcca gcacgccagc cgatgcgggt tccggcacgg tagattgctc
     3901 gattgccgtc gatgccgtcg gcgtggcgat cgactgcgcc gagggaccgc tggccaggct
     3961 ggtgccgtaa ttcgtcgcta gcgcgcccag gtcccccacg tcgatcgtgc cgttgtaatc
     4021 gaaatccccc gcggtccact cgggcatcgc gctattgtag ttcgtcgcga gccgtccgag
     4081 atccgccacg tcaacgctgc cgtcgagatt cgcatccccc gccagcgtga aacgcacctc
     4141 cgtcacaccg ccgccttggt cggcgtaacc gataacgccg ccggtgctgc tggtaaagat
     4201 ttgatgcgcg acgatgagcg cgcgaatgtc cggcgcagtc gcgttggtca gcagttcgtt
     4261 gttgccaagg tccagcttcg ccgaagtgtc ggcgaagctc agtgtccccg cgttcaacac
     4321 cgcgggcgcc gcggggaatg cggaggcgac gaggttggcg gtgacgttgg cgctgatatt
     4381 cagcgcgccg atcgtccgcg ccaacgaccc agtgccggcg ttggagccaa agctcaagct
     4441 gccgctgtcg ttcacggttg tggtgggtga caacgagccg ttga
//
LOCUS       DATIMH010000010         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4910763, whole genome shotgun sequence.
ACCESSION   DATIMH010000010 DATIMH010000000
VERSION     DATIMH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4910763"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..468
                     /locus_tag="VLI90_00170"
     CDS             73..468
                     /locus_tag="VLI90_00170"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HSV12642.1"
                     /translation="MPPNRWMFIRRALRLRCPECGVSRIFKPWRQTRSLDDWFHTLHG
                     CPRCGYPYEREQGYFLLAIWGVNYGLVAGIGVIITYTLDALYAPPIWKQIVFVIAPLP
                     IFSLLFARHSKALYLAMDHYYDPVKPDAR"
     gene            512..760
                     /locus_tag="VLI90_00175"
     CDS             512..760
                     /locus_tag="VLI90_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12643.1"
                     /translation="MDLTNPKLIYLKAALFVLGGALSAGILLAENPTLKTAALMAIAI
                     WCFARAYYFAFYVIEHYVDPTYKFAGLGSFVRYLARRR"
     gene            complement(779..1420)
                     /locus_tag="VLI90_00180"
     CDS             complement(779..1420)
                     /locus_tag="VLI90_00180"
                     /inference="COORDINATES: protein motif:HMM:NF016798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUN14 domain-containing protein"
                     /protein_id="HSV12644.1"
                     /translation="MTEQNVPPPQAQGSGVGHVIRTLPSWKRKLIGISLVMGAVGAGG
                     QLASSSLQSHPTPPVNSSGTPAANATPTDAPPAPNGARGFVSSTPAPKGDAPATPPAI
                     VGQDTSPTMTQRVSPWMTRVGLSFFVGIVVGLVSRMFLKIAAGITVLIGGVILALSYF
                     HVLNVDMSSVKTEYASASAWLGDQAVRLKDMVLHALPSTSSAAAGFLSGFKRR"
     gene            1511..2245
                     /locus_tag="VLI90_00185"
     CDS             1511..2245
                     /locus_tag="VLI90_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSV12645.1"
                     /translation="MNRFALPALPALPAMLLLLIGAQAWGQGMSAAMPTAIKPLYVKP
                     DDADWAAMLPGPPADDSPEHRAEVATLLHWQEKRTSEDVARCQSEEEVTAFVFADVLG
                     DWFNAYSLPVTANLMHQAYVDTRGVTNAAKKKWNRVRPPLAIPQIKPCVALEKSASYP
                     SGHAARGIVWATILSEIFPDHREALMARGRLIGDDRFIAGMHYPTDVAAGQKLGEEIA
                     RRLLDNSDFKQELQRAKDECLAGVHQ"
     gene            2333..2698
                     /locus_tag="VLI90_00190"
     CDS             2333..2698
                     /locus_tag="VLI90_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12646.1"
                     /translation="MPVLIKSPTRIEAAGNKPKLIEEFIGRVNSGEDRLSIARMTSPS
                     GWVEPGQRPEFDEFTIVLAGMLRVTHEGGAMEVRAGQAVLTRAGEWVQYSSPEPAGAQ
                     YVAVCLPAFSPATVHRDES"
BASE COUNT          490 a          906 c          884 g          495 t
ORIGIN      
        1 tgacccctaa aagcgccgca gcgcgccgca tttgcctcca cacccgcggt ggcggcctta
       61 gaatgccgca tcatgccgcc caaccgttgg atgtttatcc gccgagcgct ccgcctgcgc
      121 tgccccgagt gcggcgtctc ccgcatcttc aaaccctggc gacaaacgcg ttcgctcgac
      181 gactggttcc acacgctgca cggctgcccg cgctgcgggt atccctacga acgcgagcag
      241 ggctacttcc tgctggccat ctggggcgtg aactacggcc tcgtcgccgg catcggtgtc
      301 atcatcacct atacgctcga cgcgctttat gccccgccga tttggaagca gatcgtcttc
      361 gtcatcgcgc cgctgccgat cttcagcctc ctgttcgcgc ggcacagcaa ggccctctac
      421 ctggcgatgg atcattacta cgatccggtg aaaccggacg cccgatgaaa gcactggatg
      481 accgagccgt catcctaacg gcggtgcccc tatggacctc accaacccga agctgattta
      541 tctgaaggcc gcgctcttcg tcctcggcgg cgcgctgtcg gcgggaatct tgctggcgga
      601 aaatccaacg ctgaagacgg cggcattgat ggccattgcc atctggtgtt tcgcccgcgc
      661 gtattacttc gcgttctacg tgatcgagca ctacgtcgat cccacctaca aattcgcagg
      721 tcttggctcc ttcgtccgct atctcgcgcg ccgccggtga agcttcgatt actgctcgtt
      781 accgccgctt gaaccccgag agaaatccgg cggctgcgga cgacgtcgat ggcagcgcgt
      841 gcagaaccat gtccttgagc cgcaccgctt gatcgcctag ccacgccgat gctgatgcgt
      901 actcggtctt caccgacgac atgtcgacgt tcagcacgtg gaagtaggac agcgccagga
      961 tcacgcctcc gatcaacacc gtgatgcccg cggcgatctt caggaacatt cgggacacga
     1021 gccccacgac gattccgacg aagaagctca ggccgacgcg cgtcatccac ggcgagacgc
     1081 gctgcgtcat cgtcggcgac gtgtcctgac ccactatggc tggcggggtc gcgggcgcat
     1141 cgccttttgg cgccggagtc gatgagacaa acccgcgcgc cccattcggc gccggcgggg
     1201 cgtcggtcgg cgtcgcgttc gccgcgggag tgcccgatga attcaccggc ggagttggat
     1261 gcgactgaag cgatgacgac gccagctgtc cgcccgcgcc gaccgcgccc atcaccaatg
     1321 aaatcccgat cagctttcgc ttccagctcg gcagcgtgcg gatcacatgg ccgacgccag
     1381 aaccttgggc ctgcggcggc ggaacgtttt gttcggtcat gaacacctcg cgatgttggt
     1441 ctgcggaacg gatcgccggt attgtacccg cgcaggagac gcaaatatgg cgaatgttcg
     1501 tggaatgagg atgaatcgat tcgcgctgcc ggcgctgccg gcgctgccgg cgatgttgct
     1561 gctgctgatc ggcgcacagg cgtggggcca gggcatgagc gcggccatgc ccaccgcgat
     1621 caagccgctg tatgtgaagc cggacgacgc ggattgggcg gcgatgctgc cgggtccacc
     1681 ggcggatgat tcgcccgagc atcgcgcgga ggtcgcgacg ctgctgcact ggcaggagaa
     1741 gcgcacgtcc gaagacgtcg cgcgatgcca atccgaagaa gaggtgacgg cgttcgtctt
     1801 cgcggacgtg ctgggcgatt ggttcaacgc gtattcgctg ccggtcactg cgaacctgat
     1861 gcaccaggct tacgtggaca cgcgtggcgt gaccaacgcg gccaagaaaa agtggaaccg
     1921 cgtgcgcccg ccgctggcga ttccccagat caagccctgc gtcgcgctgg aaaagagcgc
     1981 gtcatacccc agcggccacg cggcccgcgg catcgtctgg gccaccatcc tgtccgagat
     2041 ctttcccgat caccgcgaag cgctgatggc ccgcgggcga ttgatcggcg acgaccgctt
     2101 catcgcgggc atgcattatc ccaccgacgt cgccgcgggg cagaagctgg gcgaagagat
     2161 cgcccggcgc ctgctggaca actccgattt caagcaggaa ctgcagcgcg cgaaggacga
     2221 atgcctggcg ggcgtgcatc agtgatgtgt agcagcgccg ggccgtcccc ggcccggacc
     2281 gggccgagga cggcccggct ccccttggct tttcacaatc ggatgaattg gtatgccggt
     2341 cctgatcaaa tcaccgacgc gcatcgaggc ggcggggaac aagccgaagc tgatcgagga
     2401 attcatcggc cgggtgaaca gcggtgaaga ccggctgagc atcgcgcgga tgacgagtcc
     2461 gagcggctgg gtcgaacccg gccagcggcc ggagtttgat gagttcacca tcgtcctcgc
     2521 gggcatgctg cgcgtgacgc acgaaggcgg ggcgatggag gttcgggccg gccaagccgt
     2581 gctgacccgc gcgggcgagt gggtgcagta ttcatcgccc gagccagcgg gcgcgcagta
     2641 cgtcgccgtc tgtctccctg cgttttcccc ggcgacggtg catcgagatg aaagttgagc
     2701 tttgcccgtt ttgcgatcaa ttacgggcga ttagcatatc tccgcacgcc gccgcttgcg
     2761 gcttcgcaat cccag
//
LOCUS       DATIMH010000011         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_579535, whole genome shotgun sequence.
ACCESSION   DATIMH010000011 DATIMH010000000
VERSION     DATIMH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579535"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="VLI90_00195"
     CDS             complement(<1..1556)
                     /locus_tag="VLI90_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSV12647.1"
                     /translation="MLLFGKSMRIHLPSPTVFRILGLSGLIFTSAFSQTATTYTITTI
                     AGTGTAGFSGDAGAATSAQISLPVGATLSGGNLYIADQANQRIRIVGSDGKINTFAGS
                     GLGGYNGDGGKATAATMNSPSNIAVDSSGTVYFTDTNNNVVRKVTKDGNIATAAGNQT
                     NNTTFGGDGGLATNASLYRPSGVAVDAAGNVYIADTTNNRIRKITVSTGIITTIAGST
                     SGGYSGDGKAAVNASLKNPVAVAVDFSGNIYIADSGNHVIRKITGTIITTVAGNGQPG
                     FSGDNGPAFAVAQLNNPKGVAVDATGNVFIADTNNGRIRMVSPAGVITTVAGTGEFGY
                     GGDGGAALSAQLNFPAGITLDASGNIYVADTSNNVIRLLKPSSSGGGAQLPVVSAGGV
                     ITASNFGASTTVAPGTWVEIYGSNLGSTARSWAGADFTNGGQNAPTSLDRTEVNIGGQ
                     KAAIDYVSPGQINVQVPFVNQGSVPLVVTTAGGNSAIYNLNVDVSKFAVYAPPQFKVG
                     DKQYVGAVLAT"
     gene            1669..>2369
                     /locus_tag="VLI90_00200"
     CDS             1669..>2369
                     /locus_tag="VLI90_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV12648.1"
                     /translation="MAAQSAAQPQRVSPNDRIRIALIGAGGQGSGDARNSLAVGGVEL
                     VAVSDIYDGRLARAREVWGDHLFTTRDYREVLARPDVDAVIIATPDHWHQKIAVDAMN
                     AGKDVYCEKPMVQLVEDGKPVVEAQKRTGRILQVGSQRVSSIVYQKAKDLLSSGAIGE
                     LNFVEAWIDRGTALGAWQYSIPLDASPATVDWDRFLGRAPKVPFEPVRLFRWRNYRDY
                     GTGVGGDLFVHLFSGL"
BASE COUNT          402 a          738 c          757 g          472 t
ORIGIN      
        1 gtggccagga ccgctccaac atactgcttg tcgcccacct tgaactgcgg cggcgcgtac
       61 accgcaaatt tggacacatc gacgttgagg ttgtagatgg cgctattgcc gcccgcggtc
      121 gtgacgacca gcggcacgct accctgattg acgaagggca cctgcacgtt gatctggccc
      181 ggactgacgt aatcgatcgc ggccttctgt ccgccgatgt tgacctcggt ccggtccagc
      241 gaggtcggcg cattctggcc gccgttggtg aaatccgcgc ccgcccacga acgcgccgtc
      301 gaacctagat tggagccgta gatttcgacc cacgtcccgg gtgcgaccgt cgtcgacgcg
      361 ccgaagttgc tcgcggtaat cacgcctccc gcgctgacta ccggcaattg cgcgccgccg
      421 ccggagctcg acggcttcag cagccggatg acgttgttcg acgtgtcggc gacatatatg
      481 ttgccgctgg catccagcgt aatgccggcc gggaagttga gctgcgcgct gagtgccgcg
      541 ccgccgtcgc ccccgtaacc gaactcgccg gtgcctgcga cggtggtgat cacgcccgcg
      601 ggagaaacca tgcggatgcg gccgttgttt gtgtccgcga tgaagacatt gcccgttgcg
      661 tccacggcca cgcccttcgg attgttgagc tgcgccactg caaacgccgg cccgttatcg
      721 cctgaaaatc cgggctgccc gttgccggcc actgtagtga tgatagtgcc ggtgattttg
      781 cggatcacgt gattgccgct gtcggcgatg tagatgttgc cgctgaaatc caccgctacc
      841 gctaccgggt tcttcaaact ggcgttcacg gcggctttcc cgtctcccga gtagcccccg
      901 ctggtgctgc ccgcgatggt cgtgatgatg cccgtggata cggtgatctt acggatgcga
      961 ttgtttgtgg tgtcggcgat atagacgttg ccggccgcat ccacggccac tcccgagggc
     1021 cggtatagcg aggcattcgt agccaggccg ccatcgccgc cgaaagtcgt gttgttggtc
     1081 tggttgccag cggcggtcgc gatattgccg tccttggtca ccttgcggac cacattatta
     1141 ttggtgtcgg tgaagtagac cgttccgctc gaatcgaccg caatgttcga cggactgttc
     1201 attgtggccg cggtcgcctt gccgccatcg ccgttgtagc cgccgagtcc cgatccggca
     1261 aacgtgttga tcttgccgtc cgaacccacg atgcggattc gctgattcgc ctgatcggcg
     1321 atatagaggt tgccgccgct cagcgtggcg cccaccggca ggctgatttg ggccgaagtc
     1381 gcggcgcccg cgtcgccgct gaaaccggcg gtgccggtgc cggcgatggt cgtgatggtg
     1441 tacgtagtcg ccgtttgact gaatgcggaa gtgaaaataa gaccggagag tcccaaaatc
     1501 cggaagacag taggggacgg aagatgaatt cgcatgcttt ttccgaacag taacacattg
     1561 acgttggttc aggctgtcaa gtttcgcgat tgtcagtaga attgaggctt aaaggaggtc
     1621 cctatgccta tcagcagacg gcattttctg ggcgcggccg gcgccggaat ggccgcgcag
     1681 tccgctgcgc agccgcagcg cgtgtcgccc aacgatcgca tccgcatcgc cctgatcggg
     1741 gccggcggac agggcagcgg cgatgcccgc aattcactgg cggtcggcgg agtcgagctc
     1801 gtcgcagtca gcgacatcta cgacggccgc ctggcgcgcg cccgcgaagt ctggggcgat
     1861 cacctcttca ccactcgcga ttatcgcgaa gtgctggcgc ggcccgacgt ggacgcggtg
     1921 atcatcgcca cgcccgacca ctggcaccag aaaatcgcgg tggacgccat gaacgcgggc
     1981 aaggatgtct actgcgaaaa gcccatggtg caactggtgg aggacggcaa gccggtggtg
     2041 gaagcccaga agcgcaccgg tcgcatcctg caagtgggca gccagcgggt cagttctatc
     2101 gtgtaccaga aggccaaaga tctgctgtca tccggcgcca tcggcgagct gaatttcgtg
     2161 gaggcgtgga tcgatcgcgg caccgcgctg ggcgcctggc aatattccat tccgctcgac
     2221 gcctcgcccg ccacggtgga ttgggaccgc ttcctgggac gcgcccccaa agtgccgttc
     2281 gagcccgtgc ggcttttccg atggcgaaac tatcgcgatt acggcaccgg cgtcggcggc
     2341 gatctcttcg tccatctctt ctccggcct
//
LOCUS       DATIMH010000012         3292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3756548, whole genome shotgun sequence.
ACCESSION   DATIMH010000012 DATIMH010000000
VERSION     DATIMH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3756548"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3292
                     /locus_tag="VLI90_00205"
     CDS             <1..>3292
                     /locus_tag="VLI90_00205"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HSV12649.1"
                     /translation="LRIDWSATKTGQHFVLDGSTSTSALGAFHNFENFYLSAGSGDDV
                     ITTGSADDNISGGGGNDIISAGEGVNAVSAGLGDDTITTGAGNDSLTGGQGNDAITGG
                     DGADTIHGDDSVWSSPGDGNDTVDAGAGNDTVYGNGGNDVISGGTGNDYLMGGRGSDH
                     VDGGDGNDYLDGYGEYGYGPDDNLPDYLNAGAGDDTLNAGYGDSVDGGAGTDQLYFNA
                     ITATAGVSANFAQLTSGGTITVGGVTLTGIEYVNNIVGTNFNDAIVAGATIGGGGIYI
                     DGYGGDDSLTGTAGLDHISGGDGSDTIIGKGGGDVLTGGAGGDTFIGTAATLNGVSIT
                     DFGAGDKIIITDASLDSVNLTLSYNLLSYNGGSLSFGRQLPGHLTVAAAAGGGVEIDI
                     QTPTLGTMDQIANQLTTGYWDGDIHHWDVTAGGTLTVDINALSSAEKTLARTALSEWS
                     NLLGIHFQEVTSGAEITFSDAESEDGGVAQTTPEWSNGYMTLAAIQISSSWIADYGSG
                     LDSYSYQTYLHEIGHALGLGHPGDYNVDASYPYDAKFTNDSWATTVMSYFSQSDNNTT
                     GFSVENAVTPMQADIIAMQQLYGLSATDHAGDTTYGYHSNVGGVYDASLYPNVALTIY
                     DSGGNDTLDYSLTSAAETINLGDATFSNVGGHFGNVAIARGVVIENALTGSGDDTVIQ
                     NAANNVLNGGGGSDTLSYATATGGITLNLGLGTAQNTGSAGFDTVLNFEKLVGTDYND
                     VLTADGSTSTLTAGGGDDILIAGMYSAVGGIGLFGGDGNDTFYLGMGSAWVDGGAGFN
                     IVDCSRATAGMLLATSGSYGAGIDTLSHIQEIIGSNYADTLSSWLPGDILIGGSGDDS
                     LSSAGGGATLAGGLGNDTYMPSDATDQIVEAAGEGIDTIVSHAANYTLSANVENLALA
                     EYNNSDPYAGAAVPPPGGYVENWNGTGNESVNVMTGNGGANSLSGMGGDDLLAGLGGV
                     DTLTGGSGNDTFLDTTSGHNGDTITDFSSNDRIVFSNANLSAFGFSISGNVLTYSGGS
                     LTLGSVPAGRLIAEAAPGGGVQIVVDRDVHNDFDGDGRSDILFQNSDGTVTDWLGRPD
                     GTFTGNAG"
BASE COUNT          676 a         1039 c         1048 g          529 t
ORIGIN      
        1 tactgcgtat cgattggtcg gcaacgaaaa ccgggcagca ttttgtcctc gatggatcaa
       61 cgtcaacgag tgcgctcggc gccttccaca atttcgaaaa cttctaccta agcgccggct
      121 cgggcgacga cgtcatcacg acgggaagcg ccgatgacaa catttccggc ggaggcggca
      181 atgacatcat ctccgcgggg gagggcgtaa atgcggtgtc cgccggtctc ggagacgata
      241 cgatcacgac cggtgccgga aacgattcgc tgaccggggg gcaaggcaat gacgcaatca
      301 ccggcgggga cggtgccgac acgatccacg gcgacgatag cgtttggtcc tcacccggag
      361 acgggaacga caccgtcgat gctggcgccg gcaacgacac tgtttacggc aacggcggca
      421 acgatgtgat ctcgggtggc acaggcaacg actacctgat ggggggccgg ggctccgacc
      481 acgtcgatgg cggcgacggc aacgactatc tcgatggtta cggcgaatac ggttacggcc
      541 ccgacgacaa cttaccggat tacctcaacg caggagcagg cgacgacacc ctgaatgctg
      601 ggtacggcga tagcgtggat ggaggcgctg gaaccgacca gctttacttc aacgcaatca
      661 cggcaacggc cggtgttagc gccaacttcg ctcaactgac cagcggcggg acgatcacgg
      721 tgggcggcgt tacgctgacc gggatcgaat atgtgaacaa cattgtcgga acgaacttca
      781 acgatgccat cgttgccggc gcgaccatcg gcggcggcgg gatctacatc gacgggtacg
      841 gcggcgacga ctccctcacc ggcacggcgg ggctggatca catcagcgga ggcgatggta
      901 gcgacacgat catcggcaag ggcggtggcg acgtcctgac cggcggcgcc ggtggtgaca
      961 cgttcatcgg cacagcagcc acgctgaatg gcgtttcgat cacggatttc ggcgcgggtg
     1021 acaagatcat catcaccgac gccagtctcg acagcgtgaa cctgacgctg tcctacaacc
     1081 tgctgagcta caatggcggg tcgctgtcgt tcgggcggca actaccgggt catctgaccg
     1141 tcgccgccgc ggccggaggg ggtgtcgaga tcgacatcca gacgccgacg ctcggcacga
     1201 tggaccagat cgcgaaccag ctgaccaccg gttattggga tggcgacatt caccactggg
     1261 acgtgacggc aggcggcacc ctgaccgtcg acatcaatgc gctcagttca gcggagaaga
     1321 ccctggcgcg cacggcgctg agcgagtggt cgaacctgtt gggcattcat ttccaggagg
     1381 tgacctccgg agccgagatc accttcagcg acgccgagag cgaggacggc ggcgtcgccc
     1441 aaacgacgcc cgaatggtcg aacggctaca tgacgttggc ggcgatccag atctccagct
     1501 catggatcgc ggactatggc agcggcctcg acagctacag ttaccagact tacctgcacg
     1561 agatcgggca tgccctgggg cttggccacc cgggcgatta caacgtcgat gccagctatc
     1621 cctatgacgc caagttcacg aacgattcgt gggctacgac ggtcatgtcg tacttcagcc
     1681 agagcgacaa caataccacc ggcttcagcg tagaaaatgc cgtcaccccg atgcaggcgg
     1741 acatcatcgc catgcagcag ctgtacggct tgtcggcaac cgatcacgcc ggcgatacca
     1801 cttacggcta ccactccaac gttggcggcg tgtacgatgc gagcctgtat ccgaacgtcg
     1861 cgctgacgat ctacgacagc ggcggcaacg acaccctcga ctactcgctg acgagcgccg
     1921 ccgagacgat caacctcggc gatgcgacgt tttccaacgt aggcgggcac ttcggcaacg
     1981 tggcgatcgc ccgtggcgtg gtgatcgaaa acgcgctcac cggcagcggc gacgacacgg
     2041 tcatccagaa cgccgcgaac aacgtgctga acggcggcgg gggcagcgac accttgtcct
     2101 atgccacggc caccggcggc atcacgctca acctcgggct gggcaccgcg cagaatacgg
     2161 gcagcgccgg cttcgacacc gtcctgaact tcgagaagtt ggttggcacc gattacaacg
     2221 atgtcctgac cgcggacgga tcaacgtcca cgctgacggc gggcggcggc gacgacattc
     2281 tcatcgcagg aatgtacagc gccgtgggcg gcatcggcct cttcggaggc gacggcaacg
     2341 acaccttcta tctgggcatg ggcagcgcct gggtggacgg cggagccggc ttcaacatcg
     2401 tcgattgcag ccgcgccacg gccggcatgc tgctggcgac gagtggctct tacggtgccg
     2461 ggatcgacac actctcgcac attcaggaga tcatcggctc gaactacgcc gacacgctct
     2521 cctcctggtt gcccggcgac atcctgatcg gcggcagcgg cgacgacagc ctgtccagcg
     2581 caggcggcgg cgcgacgctg gccggaggtc tcggcaacga cacgtatatg ccctcggacg
     2641 cgaccgatca gatcgtcgag gccgctggcg agggcatcga cacgatcgtt tcgcacgctg
     2701 cgaactacac cctttcggcc aatgtcgaga acctcgcctt agccgagtat aacaattccg
     2761 atccgtacgc cggcgctgcc gtgccaccgc ccggcggcta cgtcgaaaac tggaacggca
     2821 ccggcaacga atcggtgaac gtcatgaccg gcaacggggg cgccaattca ctgtccggca
     2881 tgggaggcga cgacttgctg gctggcctcg gcggggtcga tacgctcacc ggcggatcgg
     2941 gcaacgacac attcctggac actacctccg gccacaatgg cgatacgatt accgacttct
     3001 cgagcaacga tcgcatcgtc ttcagcaacg caaacctgtc cgcattcggc ttcagcatca
     3061 gcggcaacgt gctcacttat tcgggcgggt cgctgacgtt gggcagcgtg ccggcaggcc
     3121 gcctgattgc cgaagcggcg cccgggggcg gcgtgcagat cgtcgtcgat cgggacgtgc
     3181 ataacgactt tgacggcgac ggtcgcagcg acatcctgtt ccagaacagc gacggcacgg
     3241 tgaccgactg gctgggtcgc cccgatggga ccttcaccgg caacgccgga aa
//
LOCUS       DATIMH010000013        11982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2890340, whole genome shotgun sequence.
ACCESSION   DATIMH010000013 DATIMH010000000
VERSION     DATIMH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 11982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11982
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2890340"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3007)
                     /locus_tag="VLI90_00210"
     CDS             complement(<1..3007)
                     /locus_tag="VLI90_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV12650.1"
                     /translation="MQVRKNGRVAMVAAAVVGGLMSRYAVGAAQTWDGGAADHNWNNA
                     VNWTNDATPAGGNATINSVDPASYPIISANSAFTPVDMFVGTGAGNAGRLDQTAGTAS
                     TGNGNWMFVGVNGSSSGSFFNVANTAATGGTTTTFGQGTGSFTSGRLYVGGADFNGNG
                     TGTLNVNTSGTITTTTGGGVSFSIGHDGTGTMNLDNGTLNGAGEFWVGAAGGANGTVN
                     MSAGTINSASWFVIGRSGTGTVNISGGTINGATGGGNMTMGSFTGATGTLNASGSGIV
                     TTPNQLFVGEGAAATVNVSGNALVSPVGGLKLGVNTGATGTVNLNGGTILTPSVTQGS
                     GAGTFNFNGGTLKASAASGAFVTGISTANVGNGGAVIDSNGFAVTIGQNLLNNGTGGL
                     TKNSAGALTLTGASTYAGNTTINGGSLILSGAGSVNSSSGVTVNNGAKFITTSSAAVS
                     PAVHIVSGGVDGTGTINTLNVDSSPTNTIANGNGGTGALTVGALNLSGAAAFSLNVAS
                     TSPAIAATTLNTSGAANAVVNAANALWTPGTYDLISYGTLGGSGFGAFTKGTITGLSG
                     RQSATLGNDTAGNFITLAIAGDNPRWSGALSNEWSTNTLANPKNWALITGGTPTDYIQ
                     GDVVLFDDNATTTSPTISIANVSPTSVQFNNATKAYTLGGAFGITGSTGLIKNGAAAL
                     TVNNSNSYTGNTTLNAGTLNINNASAIGTGTLIINGGTIDNTSGSAVALSSNNPQNWN
                     GDFTFTGSNDLNMGTAPVTMSASRTVTVSGGTFTVGAIAGPNDSLTKAGAGTLVVGGT
                     SAYSGNTTVSGGTLAVNGSIVSTTSTNIDGGAITVSGTLAAPIHNLYNGSMTVNTGGT
                     VNATNGGDTWVGRNGTASLIVNTGGTFNGNTVLIGGYNNTGGTGTATVAGTLNTANWL
                     VVGFANGGIGTLNVNGGTVNIRNNNTFGNLEIGTWDNGTTGTVNMNSGSLNLLNGSAI
                     VMSVMGNHTGDTTFNQNGGNVTFYSDNGTT"
     gene            complement(3475..4971)
                     /locus_tag="VLI90_00215"
     CDS             complement(3475..4971)
                     /locus_tag="VLI90_00215"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HSV12651.1"
                     /translation="MIQNILVLGAGSAGLIAAISIKRKIPRVNVHVVRSPSIGVIGVG
                     EATTPNFPRHLFEYLGISRKRFYALASPTWKLGIKFLWGSRGRFDYTFDVQMDTHYPE
                     LSKPTGFYCDESFENASLGGALMRLDLAFPRQPNGAPEIHANHAFHIENKKFVEILEQ
                     VARELGVTFTDGKVTGSERGPSGISAIRLEDGQRLAADFFIDCSGFRSELLGRTLAEP
                     FVSFDKSLFCDRAVIGGWTRGKDEPILPYTTAETMDCGWCWRIDHEALINRGYVYCSS
                     FISDDEAAAEFHRKNPRVTDSPQIVKFRSGCYRRLWVDNVVALGNSSGFVEPLESTAI
                     MLLCSHVQTLIDFFLHSDLQPTRTLQDLYNEITYESWQDIRGFLSVHYKFNGMLQTPF
                     WRQVRADADVSSVGALLEFYRENGPTGFCRYRLPHTRSDFGLEGYLVMLVGNQVPYDT
                     RGRIPLEEQTIWQRRKQSYVEQAKRGISSEEALSYVKHPGWKWHADPV"
     gene            complement(5155..6582)
                     /gene="sthA"
                     /locus_tag="VLI90_00220"
     CDS             complement(5155..6582)
                     /gene="sthA"
                     /locus_tag="VLI90_00220"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HSV12652.1"
                     /translation="MSQAPYDLAIVGSGPAGQKAALTAARLGRRVAIIDRADSVGGVC
                     IHTGTIPSKAIREAVLHLTGLRERSVYGDSYAVKQDITMDDLLYRARHVVRTEVDVIR
                     SQMTRNGITLLNGSASFVDAHTLRIAREQDTTQIYAANILVAVGTEPARPANVPFTPG
                     HVIDSTELLQLTDLPKSLIIVGGGVIGTEYACMLAAVGVEVTLVESRPRLLEFLDDEI
                     AENLQFRLRDMGIRLRLGESVAKIEIDGDTVEATLASNKSVRGRMLLYAIGRQGATAT
                     LNTAAAGLTPDNRGRLKVNEWYQTDVPHIYAAGDVIGFPALAATSMEQGRLAASHMFH
                     QLTEAASPLFPYGIYTLPEISMVGQTEQALTQQNIPYEVGIARYREIARGQLIGDPQG
                     LVKLLFHPQSRRLLGVHAIGTGATELIHIGQAVMASGMPIDYFVNSVFNYPTLAECYK
                     VAALDGLQRSKRAAAANEEPPALAA"
     gene            6779..7675
                     /locus_tag="VLI90_00225"
     CDS             6779..7675
                     /locus_tag="VLI90_00225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSV12653.1"
                     /translation="MLYALPAAGAAWLLRAGLDRVEHSSIWSFEIFIASAAAFIGFWS
                     IFHIRRTWVRPTQQLLEAIEQARDKEAPIEELSRITGGVTPLVPIIRELLGDVKQNRA
                     ELARLEQEMRQRLAVRTDALERKIGTLQVQATRDALTGLNNRRALEQELPRIVEQYRT
                     GGHSACLLMIDVDYFKQLNDTLGHAAGDQLLKEIGQIIRSTIREQDQAYRCGGDEFVV
                     VLQQANITAGQAMVERLESLVDALARPLRIAPAPRLSIGACALSELLDPTPQSLLEMA
                     DKRLYVIKSARKQAKNASKRAG"
     gene            complement(7742..9280)
                     /locus_tag="VLI90_00230"
     CDS             complement(7742..9280)
                     /locus_tag="VLI90_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HSV12654.1"
                     /translation="MADDVAELLKQAIAHHIARQLANAERAYRAILQVQPDHAHVHYL
                     LGHLLYETGRLQESVQHQQRAAQFRPGVFEYRFELAQMYAEARRTDDAITAYQAALAL
                     RPDAVEVLQLLGNALQDAGRFDDAVNVYHRAIALRPDIPELHNNLGNALQNLRRFDES
                     IASYSNALEQRRDYPDALNNLGCALKESGRWDEAISRFRGAIELRPEYPEAHWNVATA
                     LLTVGDLRAGWRHYEWRSHFATTPPRAFNCPQWRGELLDGRRILLHAEQGFGDTIQFV
                     RFVAEVVRCGGRIVLECQPQLVRLLKGVAEIETVVAQGEPLPPFDVHCPLLSLPLALN
                     VSLQDLPGAVSYLRAGSKGVERWAGRLAGDTRRLKVGIAWAGSSLHVNDRNRSIAADQ
                     LEVFGDVPNVAFYSLQTGGAAPPNLRLTNLTADLADFADTAALVANLDMVITVDTAVA
                     HLSAAMGKPTWILLPTPGDFRWLLHREDSPWYPTARLFRQVEPSDWREPIARVREALF
                     AMAQ"
     gene            complement(9528..>11982)
                     /locus_tag="VLI90_00235"
     CDS             complement(9528..>11982)
                     /locus_tag="VLI90_00235"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HSV12655.1"
                     /translation="GVAMLALTTLGVGSHTLTAVYSGDAEFAGGSSAALAQTISKVPT
                     NTLLVSNAPLSTIGAAVTFSATVSGIGGVPSGLVGFFDGGNVIGQAVLVNGVASMTTT
                     TLATGSHTITAMYLDDAMFAPSTSPAITQVVGIASSTTTLGSSANPALVGGTIVFSAH
                     VTSASGSPSGTVTFFEGATVLGSADVFSGFAVFSIATLGFGSHNIIASYGGTTDFASS
                     ASATLVQVVGKAATTTSTGSSANPSTYGQVVGISASIFTAFGSPTGTVTFMDGTTLLG
                     TAAVVNNLATLSIAALTSGNHTIIATYSGDANFAGSVADPLLQSVSLAPTTTSLAASS
                     GVITIGQPETFTASVSAAVPGFGGTVTFRDGANLLGVASVINGVASLTTTGLSTGNHS
                     ITAGFSGDVNFAASNSAAVAVTVNLLASSTSLDVSANPVTVGDTLTLTATVSTAGAAG
                     GSVSFFDGNTLLGSAALVNGHAVFATASLAVGGHSLTASYSGDVQYVASATSAVSLTV
                     RPASTSTSLTSSASPANFGQAVQLTAAVDSTFAGAGGTVTFRDGSTLLGVVTLSNGVA
                     TLTVANFTAGGHSLSASYSGDASHTSSTGSTAQMINRGSTATAISSSSSTSTVGQSVT
                     FTATVSSTVGSPSGSVTFTDGSTVIATIALSNGVAKLTTASLAAGGHSIVATYSGDTN
                     FNANSSAPLTQLVTAATGTIAGHVYIDTTGNGFSADDRPFVGVIVELYLDRNKNGKVD
                     GGDGSPVATSTSDASGAYTFTNVAVGQYVVQQVVPGGYTQSAPSAASYSLNVTAGSVT
                     GGLDFDDLLSTGNGKGKKK"
BASE COUNT         2047 a         3919 c         3818 g         2198 t
ORIGIN      
        1 aggtggtgcc gttgtcgctg tagaacgtga cgttgccgcc gttctggttg aacgtcgtgt
       61 cgccggtgtg gttgcccatc acgctcatga cgatggcaga gccgttcagc aggttcagcg
      121 agccgctgtt catgttgacg gtgccggtcg tgccgttatc ccaggtgccg atttcgaggt
      181 tgccgaaggt gttgttgttc cggatgttca cggtgccgcc gttaacgttc agcgtgccga
      241 tgccgccgtt ggcgaaaccg acgaccagcc agttggcggt gttaagcgta ccggcgacgg
      301 tggccgtgcc ggtgccgccg gtgttgttgt atccgccgat caggacggtg ttcccgttga
      361 acgtgccgcc ggtgttgacg atgaggctgg ctgtgccgtt gcggccgacc caggtgtcgc
      421 ctccgttggt ggcgttcacg gtgccgccgg tgttcacggt catcgagccg ttgtagaggt
      481 tgtgaatggg ggcagcgagc gtcccgctga cggtgatcgc gccgccatcg atgttcgtgg
      541 aagtggtgga gacgatgctg ccgttgacgg ccagagtccc accgctcacg gtcgtattac
      601 cgctgtaggc gctggtcccg ccgacgacca gcgtgccggc gccggccttg gtcaaactat
      661 cgttcggccc ggcgatcgca ccgaccgtga atgtgccgcc gctgacggtg acggtccggc
      721 ttgccgacat cgtcaccggg gcggtgccca tgttcaggtc attgctgccg gtgaacgtga
      781 agtcgccgtt ccagttctgg gggttgttgc tcgagagcgc gacggccgaa ccgctggtgt
      841 tgtcgatcgt cccgccgttg atgatcagcg tgccggtgcc gatcgccgag gcgttgttga
      901 tgttcaacgt gcccgcgttc agcgtggtgt tgccggtgta gctgttggag ttgttgaccg
      961 tcagtgccgc agcgccgttt ttgatcagac cggtcgaacc ggtgatgccg aacgcgccgc
     1021 cgagcgtgta ggccttggtc gcattgttga actgcacgct ggtgggcgag acgttggcga
     1081 tggagatcgt cgggctggtg gtcgttgcgt tgtcgtcgaa cagcacgacg tcgccctgga
     1141 tgtagtcggt cggcgtgccg ccggtgatca gcgcccagtt cttcggattc gccagcgtgt
     1201 tggtggacca ctcgttgctg agcgcaccgc tccagcgcgg gttgtcacca gcaatcgcca
     1261 gcgtgatgaa gttgccggcg gtgtcattgc cgagcgtggc gctttggcga ccgctcaggc
     1321 ctgtgatcgt tcctttggtg aacgcgccaa agcccgaccc gcccagcgtg ccgtagctga
     1381 tcaggtcgta cgtgccgggc gtccacagcg cgttggcggc gttgacgacg gcgttggcgg
     1441 cgccgctggt gttcagcgtc gtcgccgcaa tcgccggcga ggtgctggcg acgttcaagc
     1501 tgaacgccgc tgcgccggac aggttcagcg ccccgaccgt cagcgcgcca gtcccaccgt
     1561 tgccgttggc aatcgtgttg gtggggctgc tgtcaacgtt cagcgtattg atcgtgcccg
     1621 tgccgtccac gccgccgctg acgatgtgta ccgccgggga aaccgcggcc gagctggtgg
     1681 tgatgaactt cgccccgttg ttgaccgtga cgccggagct gctgttgacc gaaccagcgc
     1741 ccgacagaat caaactgccg ccgttgattg ttgtgttgcc cgcgtatgtg ctggcgcctg
     1801 tgagcgtcag tgccccggcg gagttcttgg tcaacccgcc ggttccgtta ttcagcaggt
     1861 tctgtccgat cgtgacggca aagccattgc tgtcgatcac cgccccgccg ttgccgacgt
     1921 tggcggtgct gatgcctgtc acgaacgcgc cgctggcggc gctggccttg agcgtcccgc
     1981 cgttgaagtt gaacgtgccg gcgccactgc cctgcgtcac gctgggcgtg aggatcgtgc
     2041 cgccgttcag gttgaccgtg ccggtagcgc cggtgttcac gcccagcttc aacccgccga
     2101 cgggcgagac cagcgcgttg ccggagacgt tgaccgtcgc cgccgcacct tcaccgacga
     2161 acagttggtt gggcgtcgtg acgatgccgc tgccgctggc gttcagcgtg ccggtggcgc
     2221 cggtgaacga acccatcgtc atgttcccac caccggtggc tccgttgatc gtgccaccgc
     2281 tgatgttaac cgtgccggtc ccgctgcggc cgatgacgaa ccaggacgcg gagttgatcg
     2341 tgccggcgga catgttgacc gtaccattgg cgccaccggc ggcgccgacc cagaactcac
     2401 cggcgccgtt cagcgtgccg ttgtccaggt tcatcgtgcc ggtgccgtcg tgaccaatgc
     2461 tgaacgaaac cccaccgccg gtggtcgtgg tgatggttcc ggaggtattg acgttgaggg
     2521 tgcccgtgcc gttgccgttg aagtccgcgc caccgacata caaccggccg gaggtaaagc
     2581 tgccggtgcc ttggccgaat gtagtcgtcg tgccgccggt cgcagcggtg ttggcgacgt
     2641 tgaagaagct gccgctactc gaaccgttga cgccgacgaa catccagttg ccgttgccgg
     2701 tcgaagccgt gccggccgtc tggtccaacc gaccggcgtt cccggcaccg gtgccgacga
     2761 acatgtccac cggcgtgaac gccgagttgg cggagatgat cgggtagctc gcgggatcaa
     2821 ccgagttgat cgtcgcattg ccccccgcgg gggttgcgtc gttggtccag ttgaccgcgt
     2881 tgttccagtt gtggtctgcc gcaccgccat cccacgtttg cgcagcaccg acggcgtagc
     2941 gggacatcaa tccgccgacc accgctgccg ccaccatcgc cactcgaccg ttttttcgaa
     3001 cctgcataat cgctcctccg ctcgcacgga tcaccgctgc tcaggcgagc gatccggggc
     3061 tggtaatgtg agaaatgtca cgcgtccgct cagacgtggc tcaccctcca agggtgtaaa
     3121 ggctaaaaag ccttcgccac caactgccgt tgaatcatca attaaggttc atctccgccg
     3181 tacgttgaac tcgaaatgcc caagccagga agtaatcttc cagacgatgg agaaatccgc
     3241 gtaagcgttt tcagcaggtg acgtcgtcct ttggaccctt ccgtccctgc tatttgaccg
     3301 tttgcttccg cgagagtgag ttcacggggg aatctaccac aatctccgcg gggccgattc
     3361 ggtattcgcg caaatctcct cggtttttgc gaatgcccac aacgcccgtc attttgcgta
     3421 ttttccgcat ggtcacgcct ccgcgccgcc gggcgcgcag gcgcagatgc ccactcagac
     3481 tggatccgcg tgccacttcc aacccgggtg cttcacgtaa cttagcgctt cttctgacga
     3541 aatcccgcgc ttggcctgct cgacgtacga ctgcttccgc cgttgccaaa tggtttgttc
     3601 ctctaaagga atccggccgc gcgtgtcgta cggcacctgg ttccctacca gcattaccaa
     3661 atatccctcc agcccaaaat cgctccgcgt gtgcggcagg cgatatcggc aaaaccccgt
     3721 cggcccgttc tcgcggtaaa actccagcag cgcccccacg ctcgacacat ccgcgtccgc
     3781 gcgcacctgc cgccagaacg gcgtctggag catcccgttg aacttgtaat ggaccgacaa
     3841 aaacccgcgg atgtcctgcc agctttcgta cgtgatctcg ttgtacagat cctgcagcgt
     3901 gcgcgtcggt tgcaggtcgc tgtgcaaaaa gaaatcgatc aacgtctgca cgtgcgaaca
     3961 gagcaacatg atcgccgtcg attccagcgg ctcgacgaac ccgctcgaat tccccagcgc
     4021 caccacgttg tccacccata gccgccggta acaccccgag cggaacttca cgatctgcgg
     4081 cgaatccgtc acccgcgggt tcttccgatg aaactccgcc gccgcctcgt catcggaaat
     4141 gaacgacgag caatacacat aaccgcggtt gatcagggcc tcgtgatcga tccgccagca
     4201 ccacccgcag tccatcgtct cggccgtcgt gtacggcaag atcggctcgt ccttcccgcg
     4261 cgtccacccg ccaatcaccg cgcgatcgca gaagagcgat ttgtcaaaac tgacgaacgg
     4321 ctcggccagc gtgcggccca gcagctcact gcggaacccg ctgcaatcaa tgaagaagtc
     4381 tgcagccagg cgctgcccgt cttccagtcg aatcgcgctg atgccgctcg gcccgcgctc
     4441 gctgccggtc accttgccat cggtgaacgt cacgcccagc tcgcgcgcca cctgctcgag
     4501 gatctccacg aacttcttat tctcaatatg aaacgcgtga ttggcatgga tctccggcgc
     4561 gccgttcggc tgccgcggaa acgccaaatc caatcgcatc aacgccccgc ccaggctcgc
     4621 gttctcaaaa ctctcgtcgc aataaaatcc cgtcggcttg ctcagctccg gatagtgcgt
     4681 atccatctgc acgtcgaacg tgtaatcaaa ccgcccgcgc gagccccaca aaaacttgat
     4741 ccccagcttc cacgtcggcg acgccagcgc ataaaaccgc ttccgcgaga tgccgaggta
     4801 ctcaaacaaa tgccgcggaa aattcggcgt cgtcgcctcg cccaccccaa tcacgccgat
     4861 cgacggcgag cgcacgacat gcacgttcac ccgcgggatc ttccgcttaa tggaaatagc
     4921 cgcgataaga cccgcgctgc ccgcgcccaa cacaaggata ttctggatca tggtcgtgaa
     4981 ttgcagggaa aaatgaaccg caaccacaat accgccaacg cgcgccccga tgcaacgcgc
     5041 gcggtgcgcg tcgatagtgc gcgctccggt cccctctccc ggggtgccgg gcgaggggga
     5101 aagagcgacc tgtgattacc ctgcatgaat ttgcgcggcg gaaagtgcgc tatctcaagc
     5161 cgccagcgcc ggtggttctt cattcgctgc cgccgcgcgc ttcgaccgct gcagcccatc
     5221 cagcgccgcg actttgtagc actcggccag cgtcggatag ttgaacactg aattgacgaa
     5281 gtaatcgatc ggcatcccgc tggccatgac ggcttggccg atgtggatca gttccgtcgc
     5341 tccggtgccg atcgcgtgca cgcccagcag ccggcggctc tgcgggtgga acagcagctt
     5401 gaccagcccc tgcgggtcgc cgatcaactg cccgcgggcg atctcgcgat agcgcgcgat
     5461 gccgacttca tacggaatgt tctgctgcgt cagcgcctgc tcggtttggc cgaccatgct
     5521 gatctccggc agcgtgtaga tgccgtacgg aaacagcggg ctcgccgcct cggtcagctg
     5581 gtgaaacatg tgcgacgccg ccaggcgccc ctgctccatg ctcgtcgccg ccagcgccgg
     5641 gaaaccgatc acatcccccg ccgcgtagat gtgcggcaca tccgtctgat accactcgtt
     5701 cactttcagt cttccgcggt tgtccggtgt caaccccgcc gcggcagtgt tcaacgtggc
     5761 ggtcgcgccc tggcggccga tggcgtacag tagcattcgg ccgcgcaccg acttgttgct
     5821 ggccagcgtt gcttcgaccg tgtcaccatc gatctcgatc ttcgcgaccg actcacccag
     5881 ccgcagccga atgcccatgt cgcgcaggcg gaactgcagg ttttcggcaa tctcgtcgtc
     5941 cagaaattcc agcaaccgcg ggcgcgattc gaccagcgtc acctcgacgc ccaccgccgc
     6001 cagcatgcac gcgtattcgg tgccgatcac cccaccgccg acgatgatca atgacttggg
     6061 caggtcggtc agctgcagca gttcggtgct gtcgatgaca tgccccggcg tgaacggcac
     6121 gttcgccggt cgcgccggtt cggtgcccac cgcgaccaag atgttggcgg cgtaaatctg
     6181 cgtcgtgtcc tgctcgcgcg cgatccgcag cgtatgcgca tcgacaaaag aggcgctgcc
     6241 gttcaacagc gtgatgccgt tgcgcgtcat ctggctgcga atgacatcga cttcggttcg
     6301 cacgacatgc cgcgcgcggt acagcaggtc atccatcgta atgtcctgct tcaccgcgta
     6361 gctgtcgccg tacaccgagc gctcgcgcag gcccgtcagg tgcagcaccg cttcgcgaat
     6421 ggctttggag ggaatggtcc cggtgtggat gcagacgccg ccgacgctgt cggcgcggtc
     6481 gatgatcgcg acgcgccggc ccagccgcgc cgccgtcaac gccgccttct gccccgcggg
     6541 gcccgagccg acgatcgcca aatcataagg tgcttgggac atgtgtatgt accggtcgta
     6601 tcggttgtgc ggcacttcgg gttgagcggg cattccagca cgcgtacgta aatttgctca
     6661 cggagcagac cgatataccg atgtcgccgc ctgcaaaatg cgggcgcaca ctacaactat
     6721 ggtcgagcac ccacgccatc cagcccccgc gtcgataggc aatatcgccg ttaatatcat
     6781 gctgtacgcg ctgcccgccg ccggcgcggc ttggttgctg cgcgcggggc tcgatcgtgt
     6841 cgagcattcg tccatctggt cgttcgaaat cttcatcgct tccgccgcgg cgttcatcgg
     6901 gttctggagc atcttccaca tccgccgcac ctgggttcgt ccgacgcaac agttgctgga
     6961 agcgatcgaa caagcgcgcg acaaggaagc gccgatcgaa gaactgtccc gcatcaccgg
     7021 cggcgtcaca ccgctggtgc cgatcatccg ggaactgctc ggcgacgtga aacaaaaccg
     7081 cgccgagctg gcgcgcctgg aacaggagat gcgccagcgc ctggccgtgc gcaccgatgc
     7141 gctggagcgg aagatcggca cgctgcaggt gcaggcgacg cgcgatgcgt taacgggcct
     7201 gaacaaccgc cgggcgctgg agcaggagct gccacgaatc gtcgagcaat accggaccgg
     7261 cggacatagc gcctgcctgt tgatgatcga cgtggattac ttcaagcagc tgaacgacac
     7321 gctcggccac gccgcggggg accagctgct gaaagaaatc ggccagatca tccgctcgac
     7381 catccgcgaa caagaccaag cctaccgttg cggcggcgac gaattcgtgg tcgtcctgca
     7441 acaagccaac atcaccgccg gccaggcgat ggttgagcgg ctcgaatcgt tggtggatgc
     7501 gctggcgcgc ccgctgcgca tcgcgcccgc cccgcggctg tcgatcggcg cgtgcgcgtt
     7561 aagcgagttg ctcgacccca cgccgcaatc gctgctggag atggcggaca agcggctgta
     7621 tgtgatcaag tcggcgcgga agcaggcgaa gaacgcaagc aagcgcgcgg gctagcgcga
     7681 tgtatggaac ctcgcaaccg cgccatttcc agtccaatcc ccggccttcc ggcctgggct
     7741 gttactgagc cattgcaaat agcgcctcgc gcacacgcgc gatcggctcg cgccaatcac
     7801 ttggctccac ttgccgaaac agtcgcgcgg ttggatacca cggactgtcc tcgcggtgca
     7861 gcagccagcg gaaatccccg ggcgtgggaa gcaggatcca cgtgggtttg cccatcgcgg
     7921 cggacaggtg cgcgaccgcc gtatctactg tgatgaccat atcgaggttg gcgaccagtg
     7981 ccgcggtatc cgcgaagtcc gctagatccg ccgttaggtt cgtgagacgc agattcggcg
     8041 gcgccgcgcc gcccgtctgc aggctgtaga acgccacgtt cggcacatca ccaaagactt
     8101 cgagttgatc ggccgcgatc gatcggttgc gatcgttcac atgcagggac gaaccggccc
     8161 aggcgatgcc gaccttcagg cgccgggtgt ccccagccag tcgcccggcc caacgttcaa
     8221 cgcccttcga gcctgcgcgg aggtaagaaa cagcgccggg aagatcttgc aaagaaacgt
     8281 tcagcgccaa cggcaggctc aaaagcgggc aatgaacgtc gaacggcgga agcggttcgc
     8341 cctgggcgac aaccgtctca atttcagcaa cgcccttcag cagccgcacc agctgcggtt
     8401 gacactccag cacgattcgt ccgccgcagc ggacgacttc ggccacgaag cgcacgaact
     8461 ggatcgtgtc gccaaaaccc tgctccgcgt gcagcaggat ccgccggccg tcgagcagct
     8521 cgccgcgcca ttgcgggcag ttgaacgccc gcggcggcgt cgtcgcgaag tgcgatcgcc
     8581 attcataatg ccgccagccg gcgcgcagat cgccgaccgt cagcagcgcg gttgccacgt
     8641 tccaatgcgc ctcgggatac tctggccgca gctcgatcgc cccgcgaaag cgagaaatcg
     8701 cttcgtccca gcgcccgctc tccttcagcg cgcaaccgag gttgttcagc gcgtccggat
     8761 aatcccgtcg ctgttctagc gcattgctat aagacgcaat cgattcgtcg aaccgccgca
     8821 ggttctgcag cgcgttgccg aggttattgt gcaactcggg gatgtccggc cgcagcgcga
     8881 tcgcccggtg atacacgttc accgcgtcgt caaaccgacc cgcatcctgc agtgcattgc
     8941 caagcagctg cagcacctcc accgcgtccg gccgcagcgc cagcgccgcc tgatacgccg
     9001 tgatggcgtc gtccgttcgc cgcgcctctg cgtacatctg ggccagctca aagcgatatt
     9061 cgaacacgcc cggccgaaac tgcgccgcgc gctgctgatg ctggactgac tcctgcagcc
     9121 ggccggtctc gtagagcaaa tgtccgagca ggtagtgcac atgcgcgtgg tccggttgta
     9181 cctgcaggat agcgcgatat gcccgctcgg cattcgccag ctgtcgcgcg atgtgatgcg
     9241 cgatcgcttg cttcagcagt tccgccacgt cgtcggccat gccgcgatca taaccaacca
     9301 ccgaaagcca atgcgaacgc acagacgcag aggccgcaga gaccagatcg agactctgct
     9361 cctacctctg cggcctccgc gtctcggagt tcaatcattg ctttcgcctg agctccacca
     9421 acacagcggc cgcaggcttt aggtcgcctg cggccactgg tgggaaaggg ggagatcgcg
     9481 ttcgcacctg ccgcggtcgc gcggacttcc gcgcgacacg caacaggtca cttcttctta
     9541 cccttgccat tgcccgtgct gagcagatca tcaaagtcca gcccgcccgt cacgctgccg
     9601 gcggtgacgt tcagcgaata actcgccgct gacggcgcgc tctgcgtata gccgcccggc
     9661 accacctgct gcacaacgta ctggcccacg gcgacgttcg tgaacgtgta cgcgccgctc
     9721 gcgtcggagg tgctcgtcgc gaccgggctg ccgtcgccgc cgtccacttt gccgttcttg
     9781 ttgcggtcga ggtacagctc gacgattacg ccaacgaacg gcctatcgtc agccgaaaat
     9841 ccgttgccgg tcgtgtcgat gtagacatga ccggcgatcg tgcccgtcgc cgcggtgacc
     9901 agctgcgtca gcggcgcgga gctgttggcg ttgaagttgg tgtcgccgct gtaggtcgcg
     9961 acgatcgaat gaccgccagc cgcgaggctc gcggtggtga gcttggcgac gccgttggac
    10021 agcgcgatcg tcgcgatgac ggtcgagcca tcggtgaacg tcaccgagcc gcttggcgaa
    10081 ccgacagtcg agctaaccgt ggcggtgaac gtgaccgact gtccgacggt cgaggtggac
    10141 gatgacgaac tgatggcggt ggcggtcgag ccgcggttga tcatttgcgc cgtcgaacca
    10201 gtgctgctgg tgtgcgaggc gtcgccgctg tagctcgcgc tgagcgaatg cccgccggcg
    10261 gtgaagttgg cgacggtcaa ggtcgcgacg ccgttgctca gcgtgacgac gccgagcagc
    10321 gtcgagccgt cgcggaacgt gacggtgccg cctgcgccgg cgaaggtcga gtccactgcg
    10381 gcggtcagct gcaccgcctg gccgaagttg gcggggctgg ccgacgacgt cagcgacgtg
    10441 ctggtggacg cggggcggac ggtgagcgag acggcgctcg tggcgctggc gacgtactgc
    10501 acgtcgccgc tgtagctggc ggtcagcgag tgaccgccga cggccagcga cgccgtcgcg
    10561 aagacggcgt ggccgttcac cagcgccgcg ctgccgagca gcgtgttgcc gtcgaagaac
    10621 gacacgctgc caccggcggc gccggccgtg gagacggtgg ccgtcagcgt gagcgtatcg
    10681 cccaccgtca ccgggttcgc cgacacgtcc agagacgtgc tactcgccaa gaggttgacg
    10741 gtgacggcga cggccgccga gttgctcgcg gcgaagttca catcgccgct gaagcctgct
    10801 gtgatcgagt gattgccggt ggagagaccg gtggtggtca agcttgccac gccgttgatg
    10861 acgctggcga cgccaaggag gttggcgccg tcgcggaagg tgacggtacc gccgaatccg
    10921 ggcaccgcgg cgctgaccga cgcggtgaac gtttcgggct gaccgatcgt gatgacgccg
    10981 ctcgaagccg ccagcgacgt ggtggtcggc gcgaggctca ccgactgcag cagcggatcg
    11041 gccaccgagc cggcgaagtt cgcgtcgccg ctgtaggtcg cgatgatcgt gtgattgccg
    11101 ctggtcagcg cggcgatgct gagcgtggcc aggttattca cgaccgccgc ggtgccgagc
    11161 aacgttgtgc cgtccatgaa cgtgacggtg ccggtcggac tgccgaacgc ggtgaagatg
    11221 ctggcggaga tgccgaccac ctggccgtag gtcgaggggt tggcgctgga gccggtggac
    11281 gtggtcgtcg cggccttgcc gacgacctgc acgagcgtgg ccgacgcgct tgacgcgaag
    11341 tcggtggtcc cgccgtagct ggcgatgatg ttgtggctgc caaagcccag cgtcgcgatc
    11401 gagaagacgg cgaagccgct gaagacatcc gcgctgccga gaacggtcgc gccttcgaag
    11461 aacgtgacgg tgccgctcgg cgagccgctg gcggaggtca cgtgcgcgct gaagacgatc
    11521 gttccaccga cgagcgcggg gttcgcagaa gaacccaacg tcgtcgtgga tgatgctatg
    11581 ccgacgacct gcgtgatggc cggcgaggtg ctcggggcaa acatcgcgtc gtccaagtac
    11641 atcgcggtga tcgtgtgtga accggtcgcc agcgtggtgg tcgtcatgct ggccacgccg
    11701 ttgacgagaa ccgcttggcc gatgacgttg ccgccgtcga agaagccgac caggccgctc
    11761 ggcacaccgc cgatgccgct gacggtggcg ctgaacgtga cggccgcgcc gatcgtcgag
    11821 agcggcgcgt tgctgaccag cagcgtgttc gtcggcacct tgctgatcgt ctgcgccagc
    11881 gccgcggacg aaccaccggc gaattccgcg tcaccgctgt acacggcggt gagcgtgtgg
    11941 ctgccgacgc cgagcgtcgt caacgccagc atcgccacgc cg
//
LOCUS       DATIMH010000014         1699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3467841, whole genome shotgun sequence.
ACCESSION   DATIMH010000014 DATIMH010000000
VERSION     DATIMH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3467841"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..>1699
                     /locus_tag="VLI90_00240"
     CDS             158..>1699
                     /locus_tag="VLI90_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12656.1"
                     /translation="MGITENLSALQAQTLDSPTIEQSQTISTSFTGATLSDTGAFPPD
                     SMGDVGPTQYIVAVNGRFRSFNKTTAVADGALNANPDTFFSSVLSPVPSGGLNFTSDP
                     RIRYDRGVGRWFIVMIDVPSSKSTSIGDLPNRVMIAVSSGATITGTGNFTFYSFQGGA
                     NEFADYPTLGIDASALYIGSNIFSPTSGAFINTNAYVVRKSSILSGGPIVMTPFTNLI
                     GSDGPNTPQGVDDFDAGATQGYFIGPSNSTGKLVLRRVSDPGGTPTLSSNIILNVAAT
                     AVPLLVPHLGNTKGNNGRLDAVDDRLMNAVIRNGHLWTAQAIAVDNTGSASGTLTRDG
                     VRWYDITNLATTPTVNQFGTVSTITANNLTTERNYWIPSIMVSGQGDVAMGFSAAGSN
                     EHINAATTGRLAGDPLNTMAAPVLYTNSTTAYNPSGDTGSTSGRRWGDYSYTSIDPSD
                     DMTMWTIQEYCNATNSYAVQVVKLLAPLPATPASASGAVTQGASNVNVVVTGNAVSGS
                     GFFDPG"
BASE COUNT          338 a          574 c          504 g          283 t
ORIGIN      
        1 gttcgtttcg gagcgcggga gaagaatgcc gaaagctatc tgtgccgctg cgcgtcgtca
       61 tcgacgcgca agctcaatcg tcaaatcaga agcgctcgag cgtcgctggc tgctcagcgc
      121 ggtcagcgtt gaaatcggcc atccgatcac gggcgcgatg ggcattaccg agaatctttc
      181 agcgctgcag gcccaaacac tcgacagccc gacgatcgaa caatcgcaaa cgatcagcac
      241 ctccttcacc ggcgcgacgc taagcgacac cggtgcattt ccgccggatt cgatggggga
      301 tgtggggcca acgcaataca tcgtcgccgt caacgggcgc ttccgctcgt tcaacaagac
      361 caccgccgtg gccgatgggg cgctcaacgc caatcccgac accttctttt catcggtcct
      421 ctcgccggtg ccttccggtg gattgaactt cacctccgat ccgcgcatcc gatacgaccg
      481 cggcgttggc cggtggttca tcgtcatgat cgacgtgccc agcagcaaaa gcacgagcat
      541 cggcgacctg cccaaccgcg tgatgatcgc cgtctccagc ggcgcgacca tcaccgggac
      601 cggcaacttt acgttctact ctttccaggg cggcgcgaac gaattcgccg actatcccac
      661 gcttggcatc gatgccagcg cgctctacat cggctccaac atcttcagcc cgacgagcgg
      721 cgcgtttatc aacaccaacg cgtacgtcgt gcgcaagagt tccatcctga gcggcgggcc
      781 gatcgtcatg acgccgttta cgaacctgat cggcagcgac ggcccgaaca cgccgcaggg
      841 cgtggacgat ttcgacgctg gcgccacgca gggttatttc atcggcccgt ccaattcgac
      901 gggaaagctg gtgctgcggc gggtgagcga tccaggcggc acgccgacac tttccagcaa
      961 catcatcctg aacgtcgccg ccaccgccgt cccgctgctg gtcccgcacc tgggcaacac
     1021 caagggaaac aacggccggc tcgatgcggt ggacgatcgg ctcatgaacg cggtgattcg
     1081 caacggtcat ctctggaccg cgcaggcgat cgccgtggac aacaccggca gcgccagcgg
     1141 cacgctgacg cgcgacggcg tccgctggta tgacatcacc aacctcgcca ccacgcccac
     1201 cgtcaatcag ttcggcaccg tctcgacgat cacggccaac aacctgacga cggagcgaaa
     1261 ctattggatc ccatcgatca tggtttcggg gcagggcgac gtggcgatgg gtttcagcgc
     1321 cgccgggtcc aatgagcaca tcaacgccgc caccaccggg cggttggccg gcgatccgct
     1381 gaacacgatg gcggcgccgg tgctctacac caacagcacg accgcgtaca acccgtccgg
     1441 cgacaccggc agtaccagcg ggcggcggtg gggcgattac tcctacacca gcatcgatcc
     1501 cagcgacgac atgacgatgt ggaccatcca ggaatactgc aacgcgacga attcttacgc
     1561 ggtgcaggtg gtcaagctgt tggcgcccct gccggccacg ccggcgtcgg ccagcggtgc
     1621 cgtcacgcag ggcgcgagta acgtgaatgt tgtagtgaca ggcaacgccg tcagcgggtc
     1681 cggtttcttc gatccaggc
//
LOCUS       DATIMH010000015         6447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1446838, whole genome shotgun sequence.
ACCESSION   DATIMH010000015 DATIMH010000000
VERSION     DATIMH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446838"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VLI90_00245"
     CDS             <1..337
                     /locus_tag="VLI90_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12657.1"
                     /translation="AQQDTSGISSVFLWSGVLIGIIIIAFLAYAQFKRWMREPETSSS
                     ATGFTLSDLRELHRQGKMTDVEFEMAKVKILGTAKKAADAMPDPLAGRRNPPHADQPA
                     SQSPERNGT"
     gene            897..2180
                     /gene="clpX"
                     /locus_tag="VLI90_00250"
     CDS             897..2180
                     /gene="clpX"
                     /locus_tag="VLI90_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HSV12658.1"
                     /translation="MTTCSFCGKSSRDVGPMVEGPSDVYICANCVDLAHNIIRQEKRK
                     MTSSAPLFTSIPSPRQINEFLDQYVVGQMYAKKALSVAVHNHYKRLSMQEPAASDPAN
                     PAPAPNKQDEIEIDKSNVLLIGPTGSGKTLLAKTLARILNVPFAIGDATTLTEAGYVG
                     EDVENILLKLLQNADYDLEAAQRGIVYIDEIDKIGKTSGNVSITRDVSGEGVQQALLK
                     MLEGTVANIPPQGGRKHPEQQYIQMDTSQILFICGGTFVGLEDIIRKRLGKRQIGFNA
                     ETAPADTANDRADVLAQVQPDDLVEFGMIPEFVGRVPLIATLEPLDVKTLINILSQPK
                     NALVKQYQKFFRIEGAELEFTPGALELIAERALKRDTGARALRAVCEEIMLDLMYKLP
                     DQTQGGKYQITEDVVEGREHLFELKPESQRRKESA"
     gene            2499..2933
                     /locus_tag="VLI90_00255"
     CDS             2499..2933
                     /locus_tag="VLI90_00255"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HSV12659.1"
                     /translation="MSANVPDAHTANGPIVLFDGVCGLCDASVKFIVDHDPSGIFRFA
                     TLQSPAARALLMQHRLNPDVLSSIVLIDAGRAYTRSTAALRIGHRLSPQALSLLSLAA
                     IVIPSPIRDAVYDFIARHRYRWFGQLDACRRPTEAEALRFIA"
     gene            complement(2977..3156)
                     /locus_tag="VLI90_00260"
     CDS             complement(2977..3156)
                     /locus_tag="VLI90_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12660.1"
                     /translation="MRSILPLLSGLVFVWLIGCGQTQRDPAGDSDTRPDYYDRRPIYD
                     NQPNDNDRHFETVDK"
     gene            complement(3182..3442)
                     /locus_tag="VLI90_00265"
     CDS             complement(3182..3442)
                     /locus_tag="VLI90_00265"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV12661.1"
                     /translation="MAKATSGGVSTGFDPAKFVLNTSGFTINNGGSFSVSESADSTEI
                     DVNYVPEPSSMALLVMGSATLLRRRRRECARLRRVDSGRRWS"
     gene            3843..5369
                     /locus_tag="VLI90_00270"
     CDS             3843..5369
                     /locus_tag="VLI90_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HSV12662.1"
                     /translation="MSASASLYRACAIAAVIVAASAQLAVATDYYLDPAGKGGFGDGT
                     NTFTTIATALGSSGVPAGSSAGTPNRLLIYPGTYNVGTTSLSYSKANVALIGTTGNAD
                     DVVITSTLDSSYNPGSGALGTTGSATLQLKGSNVSAANVTFANSTDTPYIVSTGHVAV
                     SPTGSYTGGNAQTSSAPAVALLVQGDGDAFTNVKVLGYQDSLYVKGGRAYFSNSTISG
                     DNDFIFANGTVVFDHSTANIDGDHAGGAVTAASTDKRTSNGFVFLNSTITGNSVHGNP
                     VIDSQNAANASGPAAGTMYLGRPWGWQQTGGDAAVSFINTKMTSAIKSVGWLNWNSNE
                     LNAANGKNGGNPAEDTRYGEYGSTDLLGNPIDTTGRVSWSHQFTASQAAAYSLSDIFS
                     PEATYPWYGQGYPSTDAATPGTGSPNPTDPKYSWPAFWGDRNSNNDTANSIVSATYPT
                     PGNPTSYSDPSWAAGTGWDPNNQLAAAVPEPASATLLVVAGSIVLTSRRRRRAVLAPE
                     "
     gene            5608..6030
                     /locus_tag="VLI90_00275"
     CDS             5608..6030
                     /locus_tag="VLI90_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12663.1"
                     /translation="MALAIIVTLEKELPDAAAAAAYAKAGSGKALARESDRIDSAARR
                     CSVPAITSMLSESQAALIEQLKADGFDPTKMRLPPEQWFTSAEGLKTARGLIQHITAN
                     LNDFKQPNPILRDLKAVEALLIPADAAGIRFHFTKVNL"
     gene            6027..>6447
                     /locus_tag="VLI90_00280"
     CDS             6027..>6447
                     /locus_tag="VLI90_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12664.1"
                     /translation="MIRAARVLRLIGWLALAGSVAAAPDFRQVGFSTLNALGDNGTTG
                     GGDVPPIVVRNAIELQSAVERSDIKDKEMRQRTPRVVLIAGDIDLGELKNTRGGETLT
                     DIGTVRVASYTTIAAEGSGGATIDHGIFDIHGAHNVIV"
BASE COUNT         1284 a         2171 c         1897 g         1095 t
ORIGIN      
        1 cgcccaacaa gacacttcgg gcatctcttc ggtctttctc tggtccggcg ttttgatcgg
       61 catcatcatc atcgcgttct tggcctacgc gcagttcaag cggtggatgc gcgagccgga
      121 aacctcttca tcggcgacgg ggttcacgct gtcggatctg cgcgagctgc atcggcaggg
      181 gaagatgaca gatgtggagt tcgagatggc gaaggtgaag atcctcggca cggcgaagaa
      241 ggcggcggac gcgatgccgg atccgctggc gggacggcgg aacccgccgc atgcagatca
      301 gcccgcttca caatcaccgg agcgcaacgg tacctaggag agccggctcg tcccccgggc
      361 cggaataccg caagaccggg ccgaggacgg cccggctctc cttgcgcgga gatgaattgc
      421 tcgcgcgcgt cgcgccgccg ataagacaac tgttattcgg accgctcccg ccgtcattta
      481 agtgattttc ccgtttccca tttgcgtgcg catcacgtta agatacgcgc acgtcgagcg
      541 gggcggattg gcgtcaagtc tcacccctcg accgatcgat aatcctgtgc gagcggacac
      601 tgccgcgacg catttgtggc cgggtcaatg cggttgacat tctgaccgat cctacaatga
      661 aggatcgtgc gccagcgcga tctgtcccgc gcgccgcaaa tgaaccggcc gacgtagtgt
      721 ctgcggacgt agtgtacgca gagcgtacaa cgcagcgtgt gtaacgacga gactcaagga
      781 aggcgaagcc gatggccaat aacaatcgcc ccggtaacgg cggcggcggc ggacaacagc
      841 ccccctcaaa cggttcgggc ggcaacaacc cccctggcgg atttcgaggt cgacgcgtga
      901 ccacctgcag cttctgcggt aaatcttccc gtgacgtcgg gcccatggtc gaaggaccct
      961 ccgacgtcta catctgcgcc aactgcgtgg acctggccca caacatcatc cgccaggaaa
     1021 agcggaagat gaccagcagc gctccgctgt tcaccagcat ccccagcccg cggcagatca
     1081 acgaattcct cgaccagtac gtcgtcggcc agatgtacgc gaagaaggcg ctgtccgtcg
     1141 cggtgcacaa tcactacaag cgattgagca tgcaggaacc ggcggcttcc gatccggcca
     1201 accccgcgcc ggcgccgaac aagcaggatg aaatcgaaat cgacaagagc aacgtgctac
     1261 tgatcggtcc aacggggtcg ggcaagacgc tgctggccaa aacgctggcg cgcatcctga
     1321 acgtgccgtt cgccatcggc gatgcgacga cgctgaccga agccggttac gtcggcgaag
     1381 acgtcgagaa catcctgctc aagctgctgc agaacgcgga ttacgacctg gaagccgccc
     1441 agcgcgggat cgtctatatc gacgaaatcg acaagatcgg caaaacgagc ggcaacgtca
     1501 gcatcacgcg cgacgtgagc ggcgaaggcg tgcagcaggc attgctcaag atgctcgaag
     1561 gcaccgtcgc caacatccca ccgcagggtg ggcggaagca tcccgagcag cagtacatcc
     1621 agatggacac gtcgcagatt ctctttatct gcggcggcac gttcgtcgga ttggaagaca
     1681 tcatccgcaa gcggctgggc aagcgccaga tcggcttcaa cgccgagacc gccccggcgg
     1741 atacggccaa cgatcgcgct gacgtcctgg cgcaggttca gccggacgac ctggttgaat
     1801 tcggcatgat ccccgagttc gtcggccgcg taccgctgat cgccacgctg gaacccttgg
     1861 atgtgaagac gctgattaac atcctgtcgc agccgaagaa cgcgctggtg aagcagtacc
     1921 agaagttctt ccgcattgaa ggcgctgagt tggaattcac gcccggcgcg ctggagctga
     1981 tcgccgagcg cgccttgaag cgcgacaccg gcgcccgcgc ccttcgcgcg gtctgcgaag
     2041 agatcatgct cgacctgatg tacaagctgc cggaccagac gcagggcggc aagtatcaga
     2101 tcaccgagga cgtagtcgaa ggccgcgaac acctgttcga actgaagccc gagtcgcagc
     2161 gccgcaaaga atctgcgtaa gtcacgcggc atgcctggac cagatgaacc cgtgaaagcc
     2221 cacggatagc tatccgtggg cttttccttt gcgcgcgaac aacaagcaaa ttacgagacg
     2281 cgcgccttca ggagagcacc ccgcgcgctc acatccgaat cttcgaactg gcaccgttct
     2341 tgtgcttgcc aagtatgagc gaaccgcgcc cccgcgcgcc gcgcaaagcc gccaccgccg
     2401 cctgcggccg tcgatgatcc tcaccaagat cttccgccgg ccgcgtccga taacctcggc
     2461 gattcgcccg gacgcaaaag cgagcgaata atcagttgat gtccgcgaat gtccccgatg
     2521 cccacaccgc gaacggcccg atcgtcctct tcgacggcgt ctgcggcctc tgcgacgcgt
     2581 ccgtcaaatt catcgtcgat cacgacccct cggggatctt tcgcttcgcg accttgcaat
     2641 ctcctgccgc gcgggcgctg ctgatgcaac accgcctgaa cccggacgtg ctgagcagca
     2701 tcgtcctgat cgacgccggc cgggcctaca cgcgctcgac cgccgcgttg cgcatcggcc
     2761 accgcttaag cccgcaagcg ctgtcgctgc tttctctggc ggcgattgtg atcccctcgc
     2821 ccattcgcga tgcggtctac gacttcatcg cgcgccaccg ctaccgctgg ttcggccagc
     2881 tcgacgcctg ccgccggcca accgaagccg aagcgcttcg cttcatcgcc tgaaggcacc
     2941 gaagcgtgca tgcacgcgct cacgctcact cgaaaactat ttgtcgaccg tttcgaaatg
     3001 ccggtcgttg tcgttcggct gattatcgta gatcggccgg cggtcgtaat aatccggtcg
     3061 cgtatccgaa tctcccgcgg ggtcgcgctg cgtctgcccg caaccgatca accaaacgaa
     3121 caccaggccg ctcaggaggg gaagaatgct tcgcatggct gtgagtactc cagcccaaag
     3181 cctaagacca ccgtcgcccc gaatcaacgc gccgcagtcg cgcgcactca cgtcgccgcc
     3241 ggcgaagcag cgtcgcgctc cccatcacaa gtagcgccat cgacgacggc tccggcacgt
     3301 agttcacatc gatctcagtg ctatccgccg actcagacac cgagaagctc ccgccgttgt
     3361 tgatggtgaa cccgctggtg ttcagcacga acttcgccgg atcaaacccc gtgctgacgc
     3421 cgccgctcgt cgccttcgcc accgcccagt gatacccggc gtgatcctgt cgaagccggc
     3481 gaagctgttg ttcgtcacca gggtctgaaa cgtctgcccg acggccgcca cgctcaggct
     3541 cgtgaaattc agcagatccc aatccacccc cgcggtcccg ctgccgtctg ccgaaattcc
     3601 acagattatg agcgaacgcc cggtatacgt acttctgcgg agggagagga ggctcgtaga
     3661 tatcagacac ttgcttcaag acccatttcg agagccggat ttttcgctcc aagcaggcat
     3721 ctaaaattgc caaataatga cggcgaatgt gcaattgatg cattttcggc gtctattatg
     3781 ctatccgccg cgttcgttcc acgtcagttg ctcaacgagc ttcaagagag ggagtcattt
     3841 caatgtccgc ttcggcaagt ctttatcgtg catgcgccat cgccgcggtt atcgtggctg
     3901 cgtcggctca actggccgtc gccaccgact attacctgga ccccgccggt aagggtgggt
     3961 tcggtgatgg cactaacacc ttcaccacca tcgccaccgc gttgggcagc agcggcgtgc
     4021 ctgcgggctc gtcggcgggc acgcccaatc ggctgctgat ctatcccggc acctacaacg
     4081 tcggcacgac ctcgctctcg tacagcaagg cgaacgtcgc gctgatcggc accaccggca
     4141 acgccgacga tgtcgtcatc acctccacgc tcgactcgtc ctacaacccc ggctcgggcg
     4201 cgctcggcac caccggcagc gcgacgctgc aactcaaggg cagcaacgtt tccgccgcca
     4261 acgtcacgtt cgcgaacagc accgacacgc cgtacatcgt cagcaccgga cacgtggcgg
     4321 tttcaccgac gggttcttac accggtggaa acgcgcagac ctccagcgcc ccagcggtcg
     4381 cgctgctcgt acagggcgac ggcgacgcct tcacgaatgt gaaggtgctc ggctatcagg
     4441 attcgcttta cgtcaaaggc gggcgcgcgt acttctcgaa ctcgaccatc agcggcgaca
     4501 acgatttcat cttcgccaat ggcaccgtcg tctttgacca cagcaccgcc aacatcgacg
     4561 gtgaccacgc cggtggcgcc gtcaccgccg ccagcaccga caagcgcacc agcaacggct
     4621 tcgtcttcct caacagcacg atcaccggca actcggtcca cggcaacccg gtcatcgact
     4681 cgcagaacgc cgccaacgcc agcggtcccg ccgccggcac gatgtatctg ggccggccgt
     4741 ggggctggca acaaaccggt ggcgatgcgg ccgtctcctt catcaacacc aagatgacga
     4801 gcgccatcaa gagcgttggc tggttgaact ggaacagcaa tgaattgaac gccgccaacg
     4861 gcaagaacgg cggcaacccc gccgaagaca cgcgctacgg cgagtatggg tcgacggacc
     4921 tgcttggcaa tcccatcgac accaccggcc gcgtgagctg gtcgcaccag ttcaccgcca
     4981 gccaggcggc ggcgtactcg ctgtccgaca tcttctcccc cgaagccacc tacccgtggt
     5041 acggccaagg ctatccctca accgacgcgg ccacccccgg caccggttcg ccgaacccga
     5101 cggacccgaa gtacagctgg cccgcgttct ggggcgaccg caactccaac aacgacaccg
     5161 ccaactcgat cgtctcggcc acctatccca cgcccggcaa cccgacgtcg tatagcgatc
     5221 catcctgggc cgccggcacc ggctgggatc cgaataacca gctcgccgcc gccgtgccgg
     5281 aaccggccag cgccacgctg ctcgtcgttg ccggcagcat cgtcctcacg agccgccgcc
     5341 ggcgccgtgc ggttctcgcg cccgaatgaa ttcagccttt cgaggttcgc ccccgaccac
     5401 gccgggttcg gaggtacccc gacgacccgg attcttctgc tcgaagcgcc gcagaatccg
     5461 ggtcatcgcg agtacgctcc gaacccggcg tggatcggtc ccttgataag tctgcaaaag
     5521 cgtcggggct gtcggccggc gtaccggaaa gagggagatc gccgttggtg ttgatgttcg
     5581 acctcgcacg cggcacgata gttcgtcatg gctttggcaa ttatcgtcac gctcgaaaaa
     5641 gaactgcccg atgctgccgc cgccgcggcg tacgccaagg cgggcagcgg taaggcgctg
     5701 gcccgcgagt ccgaccgcat cgacagcgcc gcccgccgat gcagcgtgcc ggcgatcacg
     5761 tcgatgcttt cggagagtca ggccgcgctg atcgagcagc tgaaggccga tggattcgat
     5821 ccgacgaaga tgcgtcttcc gcccgagcag tggttcacct ccgccgaggg gctcaagacg
     5881 gcgcgcgggc tgatccagca catcacggcg aacctgaacg acttcaagca accgaacccg
     5941 atcctgcgcg acctgaaggc ggtcgaagcg ctgctgattc ccgccgatgc cgcggggatt
     6001 cgatttcatt tcaccaaggt gaacttgtga ttcgcgcggc ccgagtgctg cgattgatcg
     6061 gttggcttgc gctggctggc tctgtcgccg ccgcgccgga ttttcggcag gttggattct
     6121 ccacgctcaa cgcgctcggc gacaacggca cgaccggcgg cggggatgtg ccgccgatcg
     6181 ttgttcgtaa tgccattgaa ctgcaatcag ccgtggagcg aagcgatatc aaggacaagg
     6241 agatgcggca gcgcacgccg cgggttgtgc tgatcgcggg cgacatcgac ttgggcgagc
     6301 tgaaaaacac caggggtggc gaaacgctca ccgacatcgg caccgttcgt gtcgcgtcgt
     6361 acacgacgat cgccgccgag ggcagcggtg gggcgacgat tgatcacggc atcttcgaca
     6421 tccacggcgc gcacaacgtg atcgtcc
//
LOCUS       DATIMH010000016         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4912186, whole genome shotgun sequence.
ACCESSION   DATIMH010000016 DATIMH010000000
VERSION     DATIMH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4912186"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VLI90_00285"
     CDS             complement(<1..286)
                     /locus_tag="VLI90_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HSV12665.1"
                     /translation="MMATSQAEQTVGQLVVERPSRARVFERLGIDYCCGGKQPLAQAC
                     RQRELELDAVVAELQREATSAAAVDATERNWAAASLTDLCDHIEHTHHAYL"
     gene            845..1822
                     /locus_tag="VLI90_00290"
     CDS             845..1822
                     /locus_tag="VLI90_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide-diphospho-sugar transferase"
                     /protein_id="HSV12666.1"
                     /translation="MARYVYVLVTGEVNLYPTLCYLSALSLRGVDPAARITLLTDEPT
                     AAALHAASHRLLPLVDDVVAISDAGSPKLISRVLKTTMLQRVREPFIFLDVDTLAVRP
                     IADRFARPESLQMALDRFPEAPEPVFPQWVKPLYETMGWRCPTPRYFNSGVMFVKADA
                     AAERVFDEWHRRWRAYAEKTGRQNDQPALNSAIDATGTAVGVLPVAYNAMVQVKENFR
                     RGARLLHFFADRYGADEGTEYQKLIQRAQAGDALSFADIQQSMSRRWPLVEPWSVRRH
                     LVAGSFAGAWRTWRSGEARKHAARAKSEATKRPIRSAPDAPEPSPAGFD"
     gene            complement(1965..2255)
                     /locus_tag="VLI90_00295"
     CDS             complement(1965..2255)
                     /locus_tag="VLI90_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12667.1"
                     /translation="MIRILDDSLATAPVPNPRTNVLTARRRMILHRLLNGEQRRCSVA
                     ALEALRRCGWIHGPELAYQFTDTGRQIAEFSEEAGPLDRNLPIDPLLLATPK"
     gene            2283..2519
                     /locus_tag="VLI90_00300"
     CDS             2283..2519
                     /locus_tag="VLI90_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12668.1"
                     /translation="MSKDPSNDKTDSGEQQDRAHGMLAEIALGALFPFLSALAAAREG
                     AAGFAADVFVSVADGLDGSSNGTDHAGGATRGGL"
     gene            complement(2566..>2789)
                     /locus_tag="VLI90_00305"
     CDS             complement(2566..>2789)
                     /locus_tag="VLI90_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12669.1"
                     /translation="EGQKTVTQLTTTTLNDAQTRRRRWWMIAAILAVGIFRQGLTWIW
                     RRQPKTPAELRAEAKQRQKMIRRKLRGLE"
BASE COUNT          489 a          873 c          889 g          538 t
ORIGIN      
        1 tcagatacgc gtgatgggtg tgctcaatat gatcgcacag gtccgtcagc gacgccgccg
       61 cccagtttcg ttcggtcgca tcgaccgccg ccgcgctcgt cgcctcgcgc tggagttccg
      121 ccaccacggc gtccagctcc agttcccgtt gccggcacgc ctgcgccagc ggctgcttgc
      181 cgccgcagca atagtcgatg cccagccgct cgaacacgcg cgcccgcgaa ggtcgctcaa
      241 cgaccaattg cccgaccgtc tgctccgcct gtgaagtggc catcatcgca tcagctcctg
      301 tttggttaga tcaagccgtc aacggctgaa acaaaccata gccgctgcaa ttctagatat
      361 caaggtccag atagttggtt ctagaaaaat cgggcatcga aaagtgaggc ctaacgtccg
      421 ctgttgttta ttcgcctgat ctccgtcagg caacgggcgc gcgcacgttt gccccgaatg
      481 cggcggcgcg atcgaatacc aatccgccga agagacttcc ctaccctcgc ccgtgctggc
      541 gcccgaatcc cagccgtgct gggtcggggc gatcgttgcc gcggcgatga tccgggcaat
      601 gacataggct tcggccggac aatggcttgc gtaatatggc aagatgacga tattcagaaa
      661 atagaaggtt gcatttcgtt gcaaaacgtt gcatgcaacc ggattggtgc cagcggcgtc
      721 taggatcgcg ccaccagcgc aaggctcaga tgcaacccta gacggcgcgg gcggcgtcgg
      781 gatactctga tcttgcacgc acgctggtgg ccgtgcgcat gcggttggaa ggacgtctgt
      841 gtcgatggct cgatatgtct acgtattggt cacgggcgag gtcaatcttt acccgacgtt
      901 gtgctacctc tcggcgctgt ccctccgcgg cgtcgatccc gctgctcgaa tcacgctctt
      961 aacggatgaa ccaacggccg ctgcgctgca cgccgcgtcg caccggttgt tgccgctcgt
     1021 tgacgacgtc gtcgccattt cggatgcggg gtcgcccaag ctgatcagcc gggtgctgaa
     1081 gacgacgatg ctgcagcgcg ttcgtgagcc gttcattttt ctcgatgtgg acacgctggc
     1141 ggtgcggccg atcgcggatc gctttgcgcg gccggaaagt ttgcagatgg cgctggatcg
     1201 atttcccgaa gcgccggagc cggttttccc gcagtgggtg aagccgttgt acgagacgat
     1261 ggggtggcgc tgcccaaccc cgcggtactt caacagcggc gtgatgttcg tcaaagccga
     1321 cgcggcggcg gagcgcgtct ttgacgagtg gcatcggcgc tggcgcgcct atgccgagaa
     1381 gaccggccgg cagaacgatc aaccggcgct gaacagcgcg atcgacgcca ccggcaccgc
     1441 ggtgggcgtg ctgccggtgg cgtacaacgc gatggtgcag gtgaaggaga attttcgccg
     1501 gggcgcccgg ctgctgcact ttttcgccga ccgctatggc gcggatgaag gcaccgaata
     1561 tcaaaagctg atccaacgcg cccaagccgg cgatgcgctg agcttcgcgg atattcagca
     1621 gtcgatgtcg cggcgttggc cgctggtgga accgtggtcg gtccgccggc acctggtggc
     1681 cggcagcttc gccggggcgt ggcgcacgtg gcgcagcggc gaagcgcgta agcacgcggc
     1741 tcgagcgaag tccgaagcaa ccaaacgccc gataaggtcg gcgcctgacg cgccggaacc
     1801 atcacccgcg gggtttgact gatcgtccat ctccacgccg ggttcggagc ggtctcgcga
     1861 cgaccctaat ccctctttca atccttggga agaatccggg tcgtcgggag taccctccga
     1921 acccggcgtg gcttgcgatg tgattctttt ctaaaacaaa tcgcctactt cggggtggcc
     1981 agcagcagcg ggtcgatcgg caggttgcgg tccagcggac cggcttcttc ggagaactcg
     2041 gcgatctgcc ggccggtgtc ggtgaattga tacgccaact cgggcccgtg aatccaaccg
     2101 cagcggcgca gcgcttccag cgccgcgacc gagcagcggc gctgctcgcc gttcagcagc
     2161 cggtgcagaa tcatccgccg gcgcgcggtc agcacgttcg tccgcgggtt ggggacgggc
     2221 gctgtggcta aagaatcatc gagaatgcga atcatctcga aaagctcctg aaaacatcaa
     2281 cgatgtcgaa ggatccgtcc aatgacaaaa ccgattccgg cgaacagcag gatcgcgcgc
     2341 acgggatgct cgcggaaata gcgctcggtg cgctgttccc attcctgtcc gccttggcgg
     2401 ccgcgcgcga aggcgctgcg ggcttcgccg ctgacgtgtt cgtatccgtc gcggacggat
     2461 tggatggttc gtccaacgga accgaccacg ccggcggcgc cacccgaggc ggcctgtgat
     2521 gatcgtgctt gtgcggtgct catagcgatt gctcctggtc ccgagtcatt ccaatccgcg
     2581 cagcttccgg cggatcatct tctgccgctg cttggcttca gcgcgcagct cggccggggt
     2641 cttcggctga cgccgccaga tccacgtcag gccttggcga aagattccga cggccaagat
     2701 cgccgcgatc atccaccacc tgcgtctgcg agtctgagcg tcgttcaagg tcgttgtcgt
     2761 cagttgcgtc actgttttct ggccttcgc
//
LOCUS       DATIMH010000017         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2024576, whole genome shotgun sequence.
ACCESSION   DATIMH010000017 DATIMH010000000
VERSION     DATIMH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024576"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VLI90_00310"
     CDS             <1..262
                     /locus_tag="VLI90_00310"
                     /inference="COORDINATES: protein motif:HMM:NF045351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12670.1"
                     /translation="LAARAWQQFLHSGNSGGGTPDASAPRDPFDAHKVDNADVPATID
                     EIRNASTNATSQWCLNAIELLEMVGKEIPPDDPRWSSATTKP"
     gene            604..1290
                     /locus_tag="VLI90_00315"
     CDS             604..1290
                     /locus_tag="VLI90_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSV12671.1"
                     /translation="MIDGQRILLVDDHELVRASLRVLLEKLSPTAQVIGEASNGREAV
                     RLVRLFNPDLVIMDLNMPELNGIEATRQIMDFNPAIRVLALSMHDEPHGVEQMLRAGA
                     SGYVLKQSPVSELTAALAAAIDRRVYLSPRIAGSLVHDYLRWSSPQSSPKRTNGDGHR
                     SAFTALSRREREVLQLLAEGKTSKEIGAVLHVGVKTVESHRGQIMTKLNLRSIAQLTK
                     YAVREGITSC"
     gene            1716..>3183
                     /locus_tag="VLI90_00320"
     CDS             1716..>3183
                     /locus_tag="VLI90_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12672.1"
                     /translation="MNSKKRSTGHSFTNTRIGKKAPRSPIAAAVASLFKRRAMFERLE
                     DRTMLSVAGDIDQTFGVAGLRTTNFAGNGNDEAYSVALQSGGNYVVAGGTSGQTAFGL
                     SRYSSAGTLLSSTEASGIGAPGQAQAVAVLPDGGIIAAGYSFALPSNFNFTLVKYTAA
                     GAVDTSWGTSGIVVTPISNRADEAFAIAVQPTGPSSYKVIVAGGNFTSATREAFALAR
                     YNSDGSLDSSFGTGGIVTTSVGATGSGEIHALAINGSTLYAGGQAQNAGGNYDFALAR
                     YSLANGSLSSLTLTDFSSGSDDRANAMGIDSSGNVYLGGVTGGAAGNFAAAKYNSAGV
                     LDASFGSGGIVTTAFSSGLDAANGLAVQYNGKVLLSGYTTTASGESDLALVRLNSDGS
                     LDSNFGTGGKVTTPVAPVGSHAQGNAVLVDNTKAIVVGSIDNGTNTASPDDFLLARYQ
                     LNNAPTLGNSTASLTDITQGQTNNPGTQISVLANAAHLN"
BASE COUNT          583 a         1004 c         1020 g          576 t
ORIGIN      
        1 gttggcggcc cgcgcctggc agcagttcct ccacagcggc aacagcggtg gcggcacgcc
       61 ggatgcgtcg gcgccgcgcg atccgttcga cgcgcacaag gtggacaacg ccgatgtgcc
      121 ggcgacgatc gacgagatcc gcaatgcatc aacgaacgcg acaagccagt ggtgcttgaa
      181 tgccatcgag ctgttggaaa tggtggggaa ggagatcccc cccgacgatc cgcgctggtc
      241 cagcgcgacc acaaaaccct agcgcggcgt cgtcgtttca gcgaccctgc gttcatataa
      301 ggtgctcgca tgatcgcttg aggcgaccac gccgcgcgtt gcgcagatta caccgtaaag
      361 ggcaggctgg ggggctcgca ctgattgtat tgacttatgc gcataacgcc ggcgtgcgac
      421 aagatttcgc tggactttgg gccgctctct ttgcagaata gcgcccctcc aatatgggcg
      481 caggctgaca gggacagcca cgggcttttt tacattgatg ggctcgtcag cgcgccgttt
      541 gttttttgtc agcgtcagct gcctcggctg tgggtttgga cggcgcgcta ggggaacgcc
      601 aggatgatcg acgggcaacg gattcttctg gttgatgatc acgagttggt tcgcgcctcg
      661 cttcgggtgc tgctggaaaa gctaagcccg accgcgcagg tcatcggcga agccagcaac
      721 ggccgcgagg ccgtccgcct ggtgcgcctg ttcaaccccg acctggtcat catggatctg
      781 aacatgcccg agctgaacgg catcgaagcg acccgccaga tcatggactt caaccccgcc
      841 atccgcgtac tggccctgtc gatgcacgat gaaccgcacg gggtcgaaca gatgctgcgc
      901 gccggcgcga gcggttacgt cctcaagcag tcacccgtct cggaactgac cgcagcgctg
      961 gcggcggcaa tcgatcggcg ggtctatctc agcccgcgca tcgcggggtc gctggtgcac
     1021 gattacctgc gctggtcctc cccccagtcc tctccgaaac gaaccaatgg cgacggtcat
     1081 cgcagcgcgt tcaccgcgct gtcgcgccgc gagcgcgagg tgctgcagct gctggccgag
     1141 gggaagacga gcaaagagat tggcgcggtg ctgcacgtgg gcgtgaaaac ggtggaaagc
     1201 caccgcgggc agatcatgac gaagctgaac ctgcggtcga tcgcgcagct gacgaaatat
     1261 gcggtgcgcg aaggaattac gtcctgctag gtttttgaga aaattcgggt tgctcggggt
     1321 atttcccgga aacaaatcag ggttaacccc gatatgtggg agcaattata ggactagcat
     1381 ggctgagtcc aaaatgggcg gggtcattgg ggatcgcggg acctcctgtt gacgccgttg
     1441 tgggatgccg gagagggtag agaggtcact gtgaacacac ctctgcttct ttagatattg
     1501 cgcgtgaggc gcgatttgat tggctcgcgc tgcacgcaat tccggccgcc atttcgtgtg
     1561 ctgttgttgt tgttttcagg gctagtgtga ctgtcgatct cgcgcggcag ccacttcggt
     1621 tgtttttact actttcgcta agtaacgcgc gccgcactcg ctgtgcggcg cgcgcgctct
     1681 attgcatctt ggcatccagg gccagtgagg accttatgaa ttcaaagaag cgctcgacgg
     1741 gccattcctt cacgaacact cgcattggca aaaaggcgcc tcgctcgccg atcgccgccg
     1801 cggtcgcctc gctcttcaag cgacgcgcga tgttcgagcg cctcgaagac cgcacgatgc
     1861 tcagcgtcgc cggtgacatc gaccagacgt ttggcgttgc cgggctgcgg acgaccaact
     1921 tcgccggcaa cgggaacgat gaggcgtaca gcgtcgcgct gcagagcggc gggaactacg
     1981 tcgtcgccgg cggcacatcg ggccagaccg ccttcggcct ctcgcgctac agttcggcgg
     2041 gcacgctgct cagcagcacc gaggcctccg gcatcggcgc gcccggtcag gcgcaggcgg
     2101 tcgcggtgct tcccgatggc ggcatcattg ccgcgggcta ttcgttcgcg ctgccgtcga
     2161 acttcaactt cacgctggtg aagtacaccg ccgccggggc ggtcgatacc agctggggca
     2221 ccagcggcat cgtcgtcacg ccgatctcca atcgtgccga tgaagcgttc gccatcgcgg
     2281 tccaaccgac cggaccgtcg tcgtacaagg tgatcgtcgc cggtgggaac ttcaccagcg
     2341 cgacgcgcga agccttcgcc ctggcgcgat acaactccga cggctcgctc gacagcagct
     2401 tcggcaccgg cggcattgtc acgacgtccg tcggcgccac cggctcgggc gagatccacg
     2461 cgctggcgat caacggcagc acgctgtacg ccggcgggca ggcccagaac gccggcggca
     2521 attacgactt cgcgctggcc cgctacagcc tggccaacgg cagcctgtcg tcgctgaccc
     2581 tgaccgactt cagctccggt tctgacgacc gcgccaatgc gatggggatc gactccagcg
     2641 gcaacgttta cctcggcggt gtgaccggcg gggcggccgg gaatttcgcg gcggcgaagt
     2701 ataactcggc cggcgtactg gatgcgtcgt tcggttccgg tgggatcgtc acgaccgcgt
     2761 tcagcagcgg cctcgacgcc gccaacgggc tggcggttca atacaacggc aaagtcctgc
     2821 tcagcggata cacgaccacc gccagcggcg aatcggacct ggcgctggtt cgcctgaact
     2881 ccgacggctc gctcgattcg aattttggga ccggtggtaa ggtgacgacg cccgtcgcgc
     2941 ctgtcggcag tcacgcgcag gggaacgccg tcctggtcga caacactaag gcgatcgtcg
     3001 tcggcagcat cgacaacggc acgaacaccg cgtctccgga cgatttcctg ctcgcccgct
     3061 atcaactgaa caacgccccg acgctgggca acagcaccgc gtcgctgacg gacatcacgc
     3121 agggacaaac caacaaccca ggcacgcaga tttcggtgct ggccaacgcc gcgcatctga
     3181 atg
//
LOCUS       DATIMH010000018         1765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5201159, whole genome shotgun sequence.
ACCESSION   DATIMH010000018 DATIMH010000000
VERSION     DATIMH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5201159"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="VLI90_00325"
     CDS             complement(<1..1736)
                     /locus_tag="VLI90_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12673.1"
                     /translation="MARWLTVAAFCVALVSCAWTIGGTGAWIYLLVYGAALVPGLPIG
                     FALFGRNHAGGWIAAAVVGYALTAFAIWAPIAAHGPSWMTFVASWGVLSVSAWLLCRR
                     ITAPVVVLPTWSSNASAALLLVLLMTLAVATPPFAHVGRADVQGNRYYRAYFTADFVW
                     HTALTSEIAKFSMPPRNPYLARQPIHYYWTYYLLPAAVSQVGPAPVRDVQLCLKINAL
                     MTGLLLMSAVFLGAWTATGRALASATGATLGLVASSAEGLYETVTLWRRGAPLEQLRN
                     WNIDAVTAWPPFAGHRIDGLQRCLWYVPQHSMAYALGIVSLSIAAASGAASSMPAIIL
                     CGIALGCSVAFNPLVGGIFSLTYGMSIVIAAWRQPYLILRHAVAAVPVMAALLWCAAN
                     QMVEGAGGFLQFGLLGASRQRPLETLLLSLGPILVTGIAGLVVTRKLPIRPALPFALL
                     SIVSLFLMYFVRLSVDQAWMAFRAGQMMIVGLAALSARFVAASSSGVQRVIAVVVVAL
                     AVVAGAPTTIIDEFNAQDIHNLGMGPGFPWTIVVTVPQQRAYAWIRDNTPRSAVVQMD
                     ARSRERSTWTNIP"
BASE COUNT          386 a          553 c          575 g          251 t
ORIGIN      
        1 gggatattcg tccacgtcga acgctcacga gatcgtgcat ccatctggac gacggccgag
       61 cgcggcgtgt tgtcgcgaat ccacgcatag gcgcgctgct gtggcactgt gacgacaatc
      121 gtccagggaa aacctgggcc catgccaagg ttgtggatgt cctgcgcgtt gaactcgtcg
      181 atgatcgtcg tcggtgcacc agcgaccacg gccagcgcga ccacgacgac tgctatgacg
      241 cgctgaacgc cgctgctgga ggcagcgaca aagcgtgcac tgagagccgc gagtccgaca
      301 atcatcatct gccccgcgcg aaacgccatc cacgcttgat cgaccgagag acgaacgaag
      361 tacatcagga agagagaaac aattgagagc agggcgaacg gaagtgccgg gcggatcggc
      421 agtttacgtg tgacgaccag tcccgcgatt ccagtgacga ggatcgggcc gagcgacagc
      481 agcagggttt cgagcggccg ttgccgcgag gcgccaagaa gtccgaactg cagaaagcca
      541 ccagcgcctt cgaccatctg gttggcggca caccagagga gcgccgccat tacggggacg
      601 gcggccaccg catgacgaag gataaggtat ggctgcctcc atgccgcaat aacgatcgac
      661 atgccatagg tgagcgagaa aatgccgccg acgagcgggt tgaatgcgac cgagcatcca
      721 agcgcaatcc cgcagaggat gattgccggc atactggagg cggcgcctga tgccgcagcg
      781 atcgagagcg agacgatccc gagcgcgtac gccatcgaat gctgcgggac ataccacagg
      841 catcgttgca ggccgtcgat ccggtgtccg gcaaagggcg gccacgcggt caccgcgtcg
      901 atgttccagt ttctcagttg ctccaggggc gcgccacgac gccacagcgt caccgtctcg
      961 tagagtccct ccgcgctgga tgcgaccagg cccagcgtcg cgccggttgc tgaggccagt
     1021 gcgcgtccgg tggccgtcca cgcgccgagg aatacggccg acatcaacag caagccggtc
     1081 atcagcgcat tgatctttaa acagagttga acgtcgcgga ccggtgcggg acccacctgc
     1141 gacaccgccg cggggagcag gtaataggtc cagtagtagt gaatgggttg tcgcgccaga
     1201 taaggattgc gcggcggcat cgagaatttg gcgatctctg acgtgagtgc cgtatgccag
     1261 acgaaatcgg cggtgaagta ggcgcgatag tagcggttcc cctgtacatc agcgcggccg
     1321 acgtgcgcga atgggggagt tgccacggcc agagtcatca gcagcacgag gaggagcgct
     1381 gccgacgcgt tcgagctcca cgtgggcagc acgaccacgg gagcggtgat tcgacgacat
     1441 agcagccacg ccgacaccga gagcacgccc catgacgcga cgaacgtcat ccatgagggc
     1501 ccatgtgcgg cgatcggcgc ccatatcgcg aacgcggtga gggcgtaccc gacgaccgcc
     1561 gccgcaatcc atccgccagc gtggttgcgc ccaaacaagg caaacccgat cggcagaccg
     1621 ggaaccagcg ctgccccgta gaccagtaga taaatccagg ccccggtccc gccgatcgtc
     1681 cacgcgcagc tcacgagggc gacacaaaac gccgcgacag taagccaccg cgccatcacg
     1741 acggctgggc tcccggccgc tcggc
//
LOCUS       DATIMH010000019         5220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3468873, whole genome shotgun sequence.
ACCESSION   DATIMH010000019 DATIMH010000000
VERSION     DATIMH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3468873"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /gene="amrA"
                     /locus_tag="VLI90_00330"
     CDS             complement(<1..514)
                     /gene="amrA"
                     /locus_tag="VLI90_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HSV12674.1"
                     /translation="MQLTAEQSRRLLDIARDAIHRSLQGQPLNDLPQDDPALMQPAGC
                     FVSLHEVGTHRLRGCVGRLDASGPLATVVQQTAVNVLGDPRFTTNRVGLDDLPKLEID
                     ISVLSPLMPAANPLDFDLLNDGIYLIISNRAGCFLPQVARETGWTKEQLLDRLCTEKM
                     GFPARMWQHPA"
     gene            596..793
                     /locus_tag="VLI90_00335"
     CDS             596..793
                     /locus_tag="VLI90_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HSV12675.1"
                     /translation="MDFGPDQILFVVFLFIALAIIFRIVAGTARNHRRHALQTDARIC
                     RSCGQSHPAFAQFCRRCGTRL"
     gene            966..2633
                     /locus_tag="VLI90_00340"
     CDS             966..2633
                     /locus_tag="VLI90_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSV12676.1"
                     /translation="MSEDRSGGSGRADQRGDFSRQPPPRRWNVFDSATRVIGLVLAIG
                     VLYGLYFWAVRRVAVGPGQVLVLLKKDGSRSLPADQIIIPRPPDKATDTAGYDAWEKQ
                     YGDCNGILEQVYPTGVYFAFSPWDYERYVIDMGAADVPADKLGVVVKKFGAKLDTNQV
                     LADPKRDQRGPLPGTLPPGKYYQYANPYAYEVKLIEPIQVNPGFRGVVTNMAAGPARN
                     PNDYLVDNGEQGVQRHTEPEGFRYINPFEKRVTPVNIRSQRFEMTGDDSIRFPSSDSF
                     DIKMEGFVEWSVIPDKLPLIYTQYAEGNELLPLLEEKVILPYARSFCRLVGSQYSARD
                     FISGDTKLKFQQEFESRLREACEHEGIEVLQALVRDIVPPDSIKQPINEREIAKQQIK
                     TLEQQILVAKSQTELATQEAMSEQNQSIGDANKQVVTITKKAEQQRDVAVTKANQEFA
                     VAKLRLEAAQKEADATVAMGQAEANVILLQQQAQAEPLRQQVAAFGDGTSYAQFFFYQ
                     KVAPSVKSIMANADGPFADLFKQFLIHSPGAKNNASADKAKVTLAQP"
     gene            2773..4446
                     /locus_tag="VLI90_00345"
     CDS             2773..4446
                     /locus_tag="VLI90_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSV12677.1"
                     /translation="MKRIKSLIVGVIALVVHAYVGWLAVKWTSMRVFVEPGEALVVIN
                     KFGDPLPPGRVVVPKGDDEDKYKGVREDVLGPGRYFLNPIEYDHKIVPVVEISAGDPE
                     KWKWNPDGSLVDPNLAPQVGLVSMKEGKEAAGDQAIVDPGYRGIQKEVLTPGTYKINP
                     YQVEVTPVPAVVVPPGSVGVVTRLMGQDTAEVTSATLTAIKASTTAPAAAAGAQPAPS
                     RLVAGPNQRGILKDVLQPGIYYLNPRLVKVTIAPIGYDQITLDHGNNKAIRFYSNDGY
                     QVEADFTLVWGRSPSDVPNIVANIGDTHNVEQNVIEPAMKAACQNEGAKYTARELIQG
                     TTRSRFQDDLSSSLEAQVKARNIHVLLALIRDITIKDNRGKDATNGLLATIQQANIEV
                     ERDLTNQQKTITATTKANLEHALKLVDVARETVASDTNVKVANIMADGQKKAAEIDAD
                     RDLQVATINLQVAQLDAQKNLILGKAAAQVDQLKRDAEAQGAKMMVDAFGSAQAYNMY
                     TFAKSFEPKELRFIFAGPGTFWTDLKSFQEIGAGQVIQEATEGKKIVNP"
     gene            complement(4616..>5220)
                     /locus_tag="VLI90_00350"
     CDS             complement(4616..>5220)
                     /locus_tag="VLI90_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSV12678.1"
                     /translation="SITTAPMASRLKAPQRREQLITVATKQFGKFGYDATTTAAIADA
                     AGVTEPILYRHFGSKQELFIAITREMSTQTLSHWRELIGDIDDPAEQLREIARQFPAH
                     LKELGDAYRVIHNALTTSRDRKVLAVLREHYKQIETFFSGIIAAGIKRGAFRQMDVKT
                     PCWLLLNTGLGYALVTLNLSPFETFDVEEAIEFILRGLKA"
BASE COUNT         1061 a         1525 c         1722 g          912 t
ORIGIN      
        1 ccgcggggtg ttgccacatc cgcgcgggga atcccatctt ttcggtgcac agccgatcca
       61 gcaactgctc cttcgtccat cccgtctcgc gcgcgacctg cggcagaaag cagccggcgc
      121 ggttgctgat gatcaggtag atgccgtcgt tcagcagatc gaaatccagc gggttcgccg
      181 cgggcatcag cggcgagagg acgctgatgt cgatctccag ctttggcaga tcatcaagac
      241 cgacgcggtt ggtcgtgaag cgcggatcgc ctagcacgtt gacggccgtt tgctggacga
      301 ctgtggccag cgggccgctg gcgtctaatc gaccgacgca gccgcgtagg cggtgcgtgc
      361 cgacctcgtg caacgagacg aagcagccgg ccggttgcat cagagccggg tcgtcctgcg
      421 gcaagtcgtt cagcggttgg ccttgcaaag atcggtggat ggcgtcgcgg gcgatgtcca
      481 acagccggcg ggattgctcc gcggtcagtt gcattctggt atgctatcaa cttccaacaa
      541 tgcccggtac ttataatggg cagcgggcgc gggcttgggt gtaatgagca atcgcatgga
      601 cttcggcccg gatcaaattc tgttcgtcgt ctttctgttc atcgcgctgg cgatcatctt
      661 tcgcatcgtc gccggcacgg ccaggaatca ccggcgtcat gcgctgcaga cggacgcgcg
      721 aatctgccgc tcgtgcggcc agagccatcc ggcgttcgcc cagttctgcc ggcgctgcgg
      781 gacgaggctc tagcgcggga gaaaatcgaa tgacgaaatc cgaatccccg aatgaatgac
      841 gaatgaccaa atgcgaatga cgaatttgga acggctgctg ccattcgtca ttcgggcttc
      901 gccattcgtc attgattcgg ggattcgaac ttcgtcattc ggatttccca agagggaggt
      961 tcatcatgtc agaagatcgt tcgggtggat cgggccgcgc agatcagcgt ggcgacttct
     1021 cgcgccaacc gccgccgcgg cggtggaatg tgtttgacag cgcgacgcgc gtgatcggcc
     1081 tcgtgctggc gatcggcgtt ttgtacggcc tgtatttctg ggccgtgcgc cgcgtcgccg
     1141 tcggtcctgg gcaggtgctg gtgctgctca agaaagacgg ctcgcgcagc ctgccggcgg
     1201 accagatcat cattccgcgg ccgccggata aggcgaccga caccgcgggg tatgacgcgt
     1261 gggagaaaca gtacggcgac tgcaacggca ttctcgagca ggtctatccc accggcgtct
     1321 acttcgcgtt cagcccgtgg gattacgagc gctacgtgat cgacatgggc gccgccgacg
     1381 tcccggccga caagctgggc gtggtcgtga agaagttcgg cgcgaagctc gatacaaatc
     1441 aggtgctggc cgacccgaag cgggaccagc ggggtccgct gccgggcaca ctgccgccag
     1501 ggaagtatta ccaatacgcc aatccgtatg cgtacgaggt gaagctgatc gaaccgatcc
     1561 aggtgaatcc cggtttccgc ggcgtcgtca ccaacatggc ggcggggccg gcgagaaatc
     1621 cgaacgatta tctcgtcgat aatggcgagc agggcgtgca gcgccacacc gaaccggaag
     1681 gttttcggta catcaacccg ttcgagaagc gcgtgacgcc ggtgaacatc cgctcgcagc
     1741 gcttcgagat gaccggcgac gattcgatcc gctttccatc gtccgacagc ttcgacatca
     1801 agatggaagg ttttgtcgaa tggtcggtga ttcctgacaa gctgccgctg atttacacgc
     1861 agtacgccga gggaaacgag ctgctgccgc tgctggaaga gaaggtgatc ctgccgtacg
     1921 cgcgcagctt ctgccggctc gtcggcagcc aatacagcgc ccgcgatttc atcagcggcg
     1981 acacgaagct gaagttccaa caggaatttg aatcgcggct gcgcgaggcg tgcgagcacg
     2041 aggggatcga agtgctgcag gcgctggtgc gggacatcgt gccgccggac agcattaagc
     2101 agccgatcaa cgaacgtgag atcgccaagc agcagatcaa gacgctggag cagcagatcc
     2161 tcgtcgccaa gagtcagacg gaactggcca cgcaggaagc gatgagcgag cagaatcagt
     2221 cgatcggcga cgccaacaag caggtcgtca caatcaccaa gaaggccgag cagcagcgcg
     2281 acgtggcggt gacgaaggcg aaccaggaat tcgccgtcgc caagctaagg ctggaagccg
     2341 cccagaaaga ggcggacgcc acggtggcga tgggccaggc ggaggcgaac gtgatcctgc
     2401 tgcagcagca ggcgcaagcc gagccgttgc ggcaacaggt cgccgccttc ggcgacggca
     2461 cgtcgtacgc gcagtttttc ttctatcaga aggtcgcgcc cagcgtgaag tcgatcatgg
     2521 ccaacgcgga tgggccgttt gccgatttgt tcaagcaatt tttgatccat tcgcccggcg
     2581 cgaagaacaa cgccagtgcg gacaaggcga aagtgacgct ggcgcaaccc taatccggtc
     2641 ccctctcccg gtacgccggg agagggttag ggtgagggtg attttcgttt tcgcgagacc
     2701 gttcgacgtt cgaaatcacc ctcaccccag ccctctcccg gagtaccggg agagggggta
     2761 agaggtgcag caatgaagcg aattaaatcc ctgatcgtcg gtgtgatcgc gctcgtcgtc
     2821 cacgcgtacg tcggctggct cgcggtcaag tggacatcca tgcgcgtctt cgtcgagccc
     2881 ggcgaagcgc tcgtcgtcat caacaagttc ggcgacccgc tgccgccggg gcgcgtcgtc
     2941 gtgcctaagg gcgacgacga ggacaagtac aaaggtgtgc gcgaagacgt gctggggccg
     3001 gggcggtatt tcctgaaccc gatcgagtac gaccacaaga tcgtgcccgt cgtggaaatc
     3061 tcggcgggcg atccagagaa gtggaagtgg aacccggatg gctcgctggt cgatccgaat
     3121 ctcgctcccc aggtgggcct ggtgtcgatg aaggaaggca aagaagcggc gggcgatcag
     3181 gcgatcgtcg atcccggcta tcgcggtatc cagaaggaag tgctgacgcc cgggacatac
     3241 aagatcaacc cgtaccaggt ggaagtcacc ccggtgccgg cggtcgtggt gccgccgggg
     3301 tcggttggtg tcgtcacgcg cttgatgggg caggacaccg cggaagtgac cagcgcaacg
     3361 ttgacggcaa tcaaagcaag tacaaccgcg cccgccgccg ccgccggcgc gcaaccggcg
     3421 cccagccggc tggtcgcggg gccgaaccag cggggcattc tgaaggacgt gcttcagccg
     3481 ggcatctatt acctcaaccc gcgcctggtg aaggtgacga tcgcgccgat cggttacgac
     3541 cagatcacgc tggatcacgg caacaacaag gcgatccgct tttacagcaa cgatggctat
     3601 caggtcgagg ccgacttcac gctggtgtgg ggacggtcac cgtcggatgt gccgaacatc
     3661 gtcgccaaca tcggcgatac gcacaacgtc gagcagaacg tgatcgagcc ggccatgaag
     3721 gcggcgtgcc agaacgaagg ggccaagtac acggcccggg agttgatcca gggaacgacg
     3781 cggtcgcgat tccaggacga cctgtcgtcg tcgctggagg cgcaggtgaa ggcccgcaac
     3841 attcacgtgc tgctggcgct gatccgcgac atcaccatca aggacaaccg cggcaaggac
     3901 gcgacgaacg gcctgctggc cacgatccag caggcgaaca tcgaagtcga gcgcgatctg
     3961 accaaccagc agaagacgat taccgcaacg accaaggcga acctagaaca cgcattgaag
     4021 ctggtggacg tggcgcgcga gacggtggcg tcggacacca acgtgaaggt cgccaacatc
     4081 atggccgacg gccagaagaa ggcggcggag atcgacgccg atcgcgacct gcaggtcgcg
     4141 acgatcaacc tgcaggtggc gcagctggat gcgcagaaga atctcattct gggcaaggcc
     4201 gccgcgcagg tcgatcagtt gaagcgcgac gccgaggcgc aaggcgcgaa gatgatggtg
     4261 gacgcgttcg gctcggcgca ggcgtacaac atgtacacgt tcgccaagag ctttgagccg
     4321 aaggaactgc ggttcatctt cgcgggaccg gggacgtttt ggacggatct caagagtttc
     4381 caggagattg gcgcggggca ggtgattcag gaggcgacgg aggggaagaa gatcgtcaat
     4441 ccgtaatatt ctggtgcttt tccgccgctt gcggcttcgc aatccgggac cgcttagttt
     4501 gcgaagccgc aagcggcaag cgtagcaata atcctacaaa tgttacgttt gcaacaaaat
     4561 gttacgtttt ggcaggcgga gatgtaacgt aacaccgcag gctggcgggg gcgctctacg
     4621 ccttgagccc gcgcaggatg aactcaatcg cctcttccac gtcgaacgtc tcaaacggcg
     4681 acaagttgag cgtcaccagc gcgtaaccca ggcccgtgtt cagcagcagc cagcacggcg
     4741 ttttcacgtc catctgccgg aacgcgcccc gcttgatccc cgcggcgatg atgccggaga
     4801 agaacgtttc gatctgcttg tagtgctcgc gcagcaccgc cagcaccttg cgatcgcggc
     4861 tggtcgtcag cgcgttgtgg atgacgcggt aggcgtcgcc gagttctttg agatgcgcgg
     4921 ggaactgccg ggcgatctcg cgcagctgct cggccgggtc gtcgatgtcg ccgatcaact
     4981 cgcgccagtg cgacagcgtc tgcgtgctca tctcgcgcgt gatggcgata aacaactcct
     5041 gtttcgaccc gaagtggcga tagaggatcg gttcggtgac gccggcggcg tcggcgatgg
     5101 cggcggtggt cgtcgcgtcg taaccgaact tgccgaactg cttcgtcgcg acggtgatca
     5161 actgttcccg tcgctgcggc gctttgagcc tgctggccat gggcgcagta gtaatcgagc
//
LOCUS       DATIMH010000020         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_581319, whole genome shotgun sequence.
ACCESSION   DATIMH010000020 DATIMH010000000
VERSION     DATIMH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581319"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="VLI90_00355"
     CDS             complement(<1..1697)
                     /locus_tag="VLI90_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSV12679.1"
                     /translation="MMYNTTRLPVRRAARFIALAVAALCLGSCTTDPPKKIANRYVTL
                     PHKQVPEYLKDTILEYTDLGGTEPFPISGYGLVANLKGTGGSRAPAAVREFIIKDMAR
                     HGFGNVGGGLDSPEDILNNKSFAIVRVDGFLPVGARASHKDPASGEADWCTWFDVRVS
                     ALPESETASLAHGDLYATDLKVAGANPLDPGSGNVAVRAQAMGSIFINPAYALDDTTD
                     TAAARSSRRTGVVIGGAKPLENRPLFLRLRSPERRMARAIERRVNEQFQDVMDKDLPV
                     KGNTTFLCEAEDEGIVKVFVPRAYVDDWTHFAGVLKHLYLNGGLPQYATVKAEQLAQE
                     AVKPGAALMDISYALEGLGKPAMHAITPLLTNPSPEVQFAAARAAAFIGDPAAVPVLM
                     SIARTQGNPFRVNAVRALGQLPQSPMVDRLLTQLLGIDEAMVRIEAYEVLARHKDGAI
                     YSRVIKRGNNEKFMLDIVPGGGTPMVYASRQGIPRLAVFGVRTAVDLPVMFVAMNNRL
                     TISSVGDNEVVTVYYRGAELRKPVSVACGTSIAEIVAKLGGETVPGEQALDFSYADVV
                     "
     gene            complement(1840..>2438)
                     /gene="nrdR"
                     /locus_tag="VLI90_00360"
     CDS             complement(1840..>2438)
                     /gene="nrdR"
                     /locus_tag="VLI90_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSV12680.1"
                     /translation="EKESLKVIDSRACDGGRAIRRRRECLNCAKRFTTYEKIEEPVRV
                     IVVKKDGRRIPWDRNKILRGLERACFKRPVPEAELLRITDEVEDEIYRTHDREVPSEL
                     IGRLVTERLRRLDQVAYVRFASVYRQFKTLEELVEEARAVIDAQRYDDPGQGKLFIDP
                     NAPAAAAGNGEAVGGGESRKSKGKRTKAAAAGNVDPAE"
BASE COUNT          400 a          863 c          712 g          463 t
ORIGIN      
        1 acgacgtcgg cataactgaa gtccagcgcc tgctcgcccg gcacggtttc accgccgagc
       61 ttggcgacga tctccgcgat gctcgtcccg cacgccacgc tgaccggctt gcgcagttcc
      121 gcgccgcggt agtaaaccgt caccacctcg ttgtcaccga ccgaggagat cgtcagccga
      181 ttgttcatcg caacgaacat caccggcagg tcgaccgccg tgcgcacgcc aaacaccgcc
      241 agccgcggga tgccctgccg gctggcgtac accatcggtg tccccccgcc ggggacgatg
      301 tccagcatga acttttcatt gttgccgcgc ttgatcacgc gcgaataaat cgccccgtcc
      361 ttgtggcggg caagcacttc atacgcctcg attcgcacca tcgcctcgtc gatgccgagc
      421 aattgcgtga gcagccgatc gaccatcggc gactgcggca actgccccag cgcgcgaacc
      481 gcattcaccc ggaacgggtt gccctgcgtt cgcgcgatcg acatcagcac cggcaccgcc
      541 gcggggtcac cgatgaacgc cgcggcgcgc gcggcggcga actgcacctc cggcgacgga
      601 ttggtcaaca gcggcgtgat cgcgtgcatc gccggtttgc ccaagccttc cagcgcgtac
      661 gaaatatcca tcagcgccgc gccgggtttc accgcttcct gcgccagttg ctccgccttt
      721 accgtcgcgt attgtggcag cccgccgttc aaatacaggt gctttagcac gcccgcgaaa
      781 tgcgtccagt cgtccacgta cgcccgcgga acgaagacct tgacgatgcc ttcgtcctcg
      841 gcttcgcaca agaacgtcgt gttgcccttg accggcagat ccttgtccat cacgtcctgg
      901 aactgctcgt tcacccgccg ctcgatcgcc cgggccatgc ggcgttctgg cgagcgcagg
      961 cgcagaaaca gcgggcgatt ttccaatggc ttggccccac cgattaccac gcccgtccgc
     1021 cggctgctgc gcgcggcggc ggtgtcggtc gtgtcgtcca gcgcatacgc cggattgatg
     1081 aagatcgagc ccatcgcctg cgcgcgaacc gcgacattcc cgctgccggg gtccagcggg
     1141 ttcgccccag ccaccttcaa gtcggtcgcg tacagatccc cgtgcgccag gctggcggtt
     1201 tcactttcgg gcaaagccga gacgcgcacg tcgaaccacg tgcaccaatc cgcttcgccc
     1261 gaggcgggat ccttgtgcga cgcccgggcg ccgaccggca ggaagccatc gacgcgcacg
     1321 atcgcaaagc tcttgttgtt caggatgtct tccggcgaat cgagcccccc gccgacgttg
     1381 ccgaaaccgt gccgcgccat gtccttgatg atgaactcgc gcaccgccgc gggcgcccgg
     1441 ctgccgccgg tgcctttgag gttggcgacc aggccgtagc ccgagatcgg gaacggctcg
     1501 gtcccaccca agtcggtgta ttccaaaatc gtgtctttca gatactccgg cacctgcttg
     1561 tgcggcaggg tcacgtagcg gttggcgatt ttcttcggcg gatcagtggt gcagctgccc
     1621 aggcaaaggg cggccaccgc cagcgcgatg aatcgcgccg cgcgccggac aggcaggcgt
     1681 gtcgtgttgt acatcatggt tgagcgcctc cagacattat cggaccaccg accgcgccgg
     1741 ctttacagcc gcttgacttc gacgagctta gtcgagtcgc gggttagcgt ctgttttcct
     1801 gcgacataaa tgacaaatgc taagccgcaa gcggcaacgt cattctgccg gatcaacatt
     1861 ccccgccgcc gccgctttcg tgcgtttccc cttggatttc cgcgattcac cgccgccgac
     1921 cgcttcgcca tttcccgccg ccgccgcggg ggcattcgga tcgatgaaca gcttcccctg
     1981 ccccggatcg tcgtagcgct gggcgtcgat caccgcccgg gcctcttcca ccagttcttc
     2041 cagcgttttg aactgtcgat agacgctggc gaaccgcacg tacgccacct gatccaaccg
     2101 ccgcaggcgt tcggtaacca gccggccgat cagctccgac ggcacctcgc ggtcgtgcgt
     2161 gcgatagatc tcgtcttcga cttcgtcggt gatccgcagc aattccgctt ccggtaccgg
     2221 gcgtttgaag caggcccgtt ccagcccgcg caggatcttg ttgcgatccc acggaatgcg
     2281 ccggccatcc ttcttaacga cgatcacgcg gacgggttct tcaatttttt cgtaggtggt
     2341 gaagcgtttg gcgcagttca ggcactcgcg ccgccgccga atcgcccgcc caccgtcgca
     2401 ggcgcgcgaa tcgatcacct tgaggctttc cttctccg
//
LOCUS       DATIMH010000021         3651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3469107, whole genome shotgun sequence.
ACCESSION   DATIMH010000021 DATIMH010000000
VERSION     DATIMH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3469107"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VLI90_00365"
     CDS             <1..248
                     /locus_tag="VLI90_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HSV12681.1"
                     /translation="LLPVVGDTLGLLPGLYPVYVARRHHLGRRVRVQMGLNLLVEWLI
                     GITPLIGDAADVWFKANLRNLKLLERAAAAKRSMRSR"
     gene            complement(316..2529)
                     /locus_tag="VLI90_00370"
     CDS             complement(316..2529)
                     /locus_tag="VLI90_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HSV12682.1"
                     /translation="MSSTGSNPPAPSATAAAPLGDPNLSAKRQLKKKNTAEFWRATSY
                     LAPYRKLVIASIISALFVGVAMAGGFTAMIPILRVLINGDTVPNWVNRQVVQKRLQLE
                     VITDSPDLRVIRVMADGPAAVAGIIPGDALAPAGGPLDTTQVLSQLSDPEVSSMDLTL
                     ERDHRHVTIAKLPPVPVYFAAIRPMLARFPVRPVPAVAIVLAVCVLISAIGNSIRFIQ
                     EYYSDKAAILAINDIRRRMYDHVLHVPMSFFGLKGTSDVTSRLIQDCQALQDGFKTVL
                     GQSIQMPINALFAFAVALKISWKLTLFITLFAPVMVAIIQKFGKKMRRASRKALQRSS
                     SMLGQIEGTLGGIRVVKGANAERFERRRYGVIMGGLTDEQLRMSRIDAISSPVVEVAT
                     LLVVCIVVLWATHLVRTTRELTPENFIVVMACLAMIGESLRRFGKLNGVLQRSGAAAS
                     RVFETLSVPVERPRHMTGRAERPKMKLPPVAREIRFQDITFSYTNMGTPALDGVNLSV
                     RQGESVAIVGRNGSGKTTLLALLPRFYDPQQGRVTIDGVDVRDVTLKSLRDQITIVTQ
                     DSVIFPGTIAENIAYGHPLAAHLKSDTAAARELRAQIEHAAKRAFAHDFILEKPLGYD
                     TELGGLGGSLSGGQKQRINIARAILRATPILILDEATSQVDAESEHLIQKAVEGLMHE
                     RTTFVIAHRFSTILSADRIIVMDRGQIVGMGKHDELQRTCQTYVNLYERQLPPAA"
     gene            2714..>3651
                     /locus_tag="VLI90_00375"
     CDS             2714..>3651
                     /locus_tag="VLI90_00375"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSV12683.1"
                     /translation="MPLPIPLSQPDLTQREIDAVVEVLHTPTLSIGPRVEEFEAKVAK
                     LVNRRHAIAVSSGTAGLHCAMIAAGVKAGDEVITTPFSFVASANCALYVDATPVFVDI
                     DPMTLNMDVAKVPAAITSKTKAIVAVEAFGHPGGMIELEQLAQKNELILIEDSCEGFG
                     GYVGRGPTMRAIGSFGRAGVFGFYPNKQITTGEGGMIVTDDDTFAATCRALRNQGREG
                     MTWLAHQRLGYNYRLSEINAAIGVVQMNRLEEILDGRRRVAHLYIERLMTNRYLILPT
                     LQDDEHMSWFVFVVRLNDLFEPGDRDEVMKQLRAEG"
BASE COUNT          686 a         1228 c         1072 g          665 t
ORIGIN      
        1 gtctgctccc cgtcgtcggt gacacgctcg gcctgctgcc cggcctctat cccgtttacg
       61 tcgcccgccg ccaccacctc ggccgccgcg tgcgcgtgca gatgggcctg aaccttctgg
      121 tcgaatggct gatcggcatc accccgctca tcggtgacgc cgccgatgtg tggttcaaag
      181 ccaacctgcg caacctgaag ctgctcgaac gcgccgccgc cgcgaagcgc tcgatgcgca
      241 gtcgttaaac cccacgccgg gttcggagcg tacccgcgac gacccggatt cttcttttgg
      301 aaaaggaaag gcgaactacg ccgccggcgg cagctgccgc tcgtacaagt tcacatacgt
      361 ctggcacgtc cgctgaagct catcgtgctt ccccatgccg acgatctgcc cccggtccat
      421 cacgataatt cgatcggcgc taagaatcgt gctgaagcga tgcgcgatca cgaacgtcgt
      481 ccgctcgtgc atcaatcctt caaccgcctt ctgaatcagg tgctcgctct cggcatccac
      541 ttggctggtc gcctcgtcca ggatgaggat cggcgttgca cgaagaatgg cgcgcgcaat
      601 gttgatgcgc tgtttctgtc cgccggatag cgacccaccc agccccccca gttccgtgtc
      661 gtagcccagc ggtttttcca ggatgaagtc atgcgcgaac gcgcgtttcg ccgcgtgttc
      721 gatctgcgct cgcaactcgc gagcggccgc tgtgtcgctc ttcagatgcg ccgccagcgg
      781 atgcccatac gcgatgttct ccgcgatcgt gccgggaaaa ataacactgt cctgtgtcac
      841 gatcgtgatc tgatcgcgca ggctcttaag cgtcacatcg cgcacgtcca cgccgtcgat
      901 cgtcacccgc ccctgttgcg ggtcataaaa ccgcggcagc agcgccagca gcgtcgtctt
      961 cccgctgccg ttgcgcccga cgatcgcaac gctttcgccc tggcgcaccg ataaattcac
     1021 cccatccagc gccggtgttc ccatgttcgt ataactgaac gtgatgtcct ggaatcgaat
     1081 ttcccgcgcc accggcggca gcttcatctt gggccgttcg gcgcgaccgg tcatgtgccg
     1141 cgggcgctcg acgggaacgc tcagcgtttc gaacacgcgc gatgccgccg cgccgctgcg
     1201 ctgcagcacg ccattcagct tgccgaatcg gcggagcgat tcaccgatca tcgccaggca
     1261 cgccatcacg acgatgaagt tttccggcgt cagctcacgc gttgtgcgga ccaggtgcgt
     1321 cgcccacaac acgacgatgc agacgaccag gagcgtggcc acttcgacca cgggcgaact
     1381 gatcgcgtcg atccggctca tgcggagctg ttcatccgtc aacccgccca tgatcacgcc
     1441 ataccgccgg cgttcgaatc gctcggcgtt cgcgcctttg acgacgcgga tgccgcccag
     1501 ggtaccttcg atctgcccaa gcatcgacga gctgcgctgc agcgccttgc ggctggcccg
     1561 ccgcattttt tttccgaact tctggatgat cgccaccatc accggcgcaa acagcgtgat
     1621 gaacagcgtc agcttccaac tgatcttcag cgccaccgcg aacgcgaaca gcgcgttgat
     1681 cggcatctgg atcgattggc ccagcaccgt cttgaaccca tcctgcagcg cctggcaatc
     1741 ctggatcagt cggctcgtca cgtcgctcgt ccccttaagc ccgaaaaaac tcatcggcac
     1801 gtgcagcacg tgatcgtaca tccgcctgcg aatgtcattg atggcgagga tggccgcctt
     1861 gtcgctgtaa tattcctgga tgaagcggat cgagttgccg atcgcggaga tcagcacgca
     1921 caccgccagc acgatggcga cggccggcac gggccgcacc ggaaatcgcg ccagcatcgg
     1981 ccggatcgcc gcgaagtaga ccggcaccgg cggcagcttg gcgatcgtca catgccgatg
     2041 atcgcgctcc agcgtcaggt ccatcgacga cacctccgga tccgacaact gcgacaacac
     2101 ctgcgtcgta tccagcggcc cgcccgccgg cgccagcgca tcgcccggaa tgatccctgc
     2161 gaccgcggcg gggccatcgg ccatcacgcg gatgacccgc aggtccggcg aatcggtgat
     2221 gacctccagc tgcaaccgct tctgcaccac ttggcggttc acccagttcg gcacggtgtc
     2281 gccgttgatc aacacgcgca ggatcgggat catcgccgtg aatcccccgg ccatcgccac
     2341 gccgacaaac agcgccgaga tgattgatgc gatcaccagc ttgcgatacg gcgcgagata
     2401 cgaggtggcc cgccagaact ccgctgtgtt tttcttcttc aactgccgct tggcagacaa
     2461 attaggatca ccgagcggcg cggctgcggt ggcagaggga gcagggggat tggaaccagt
     2521 cgaactcatg cggtacaagc cttgtagacg atagaagcgt tatcggacgc gaagcggcgg
     2581 attgtatagc gatcggtctc gcttcgcgaa cgagcgtggc tcgaacgtga tgacgtggat
     2641 atgtcattac gtgacgagaa cgccgatggc aagttttcag ctaatcacga attcacatat
     2701 tcacgaaccg cccatgcccc tccccatccc gctttcgcaa ccggacctca cgcagcgcga
     2761 gatcgacgcc gtcgtcgagg tgctgcacac gccgacgctc tcgatcggcc cgcgcgtcga
     2821 agagttcgag gcgaaggtcg ccaagctggt gaaccgccgg cacgccatcg ccgtctccag
     2881 cggcacagca ggcctgcact gcgcgatgat cgctgcgggt gtgaaggcgg gggatgaagt
     2941 catcacaacg ccgttcagct tcgtcgcgtc ggccaactgc gcgctgtacg tcgatgcgac
     3001 gcccgtattc gtcgacatcg atccgatgac gctgaacatg gacgtcgcca aagtccccgc
     3061 ggccatcacg tcgaagacga aagccatcgt cgccgtcgaa gcgttcggcc accccggtgg
     3121 catgatcgag ctggaacaac ttgcccagaa gaacgagctg atcctgatcg aagattcttg
     3181 cgaaggcttc ggcggctacg tcggccgcgg gccgaccatg cgggcgatcg gctccttcgg
     3241 tcgcgccggt gtcttcgggt tctatccgaa caagcagatc accaccggtg aaggcgggat
     3301 gatcgtcacc gacgatgaca cgttcgccgc gacctgccgg gcgctgcgga accagggccg
     3361 cgagggcatg acgtggctcg cccatcagcg cctcggctac aactatcgcc tgagcgaaat
     3421 caacgcggcc atcggggtcg tgcagatgaa ccgcctcgag gaaatcctcg acggccggcg
     3481 acgcgtcgcc cacctgtaca tcgaacggct catgaccaac cgctacctga tcctgccgac
     3541 gttgcaggat gatgagcaca tgagctggtt cgtcttcgtc gtgcgattga acgacctgtt
     3601 cgagcccggc gatcgcgacg aggtgatgaa gcagctgcgg gccgaaggca t
//
LOCUS       DATIMH010000022         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3757948, whole genome shotgun sequence.
ACCESSION   DATIMH010000022 DATIMH010000000
VERSION     DATIMH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3757948"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..830
                     /locus_tag="VLI90_00380"
     CDS             378..830
                     /locus_tag="VLI90_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12684.1"
                     /translation="MHKKSAVVESDATAATAVESEARGQGPDRRASRVDRRTGIERRQ
                     LSAEASNYTGAERRSGTDRRDDTGLERRRGPGRRRSDERRSAEEGEMNTEQFEFVMAI
                     ETYKKVNRKMYPTWTEILEVVRQLGYRKVEHRDIQLENVPEPDLQKVA"
     gene            complement(869..2245)
                     /locus_tag="VLI90_00385"
     CDS             complement(869..2245)
                     /locus_tag="VLI90_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSV12685.1"
                     /translation="MHKTVVINAVGLTPNLIGPHTPRLAGFRERGRLASVGAVIPAVT
                     TSVQSTYLTGAWPNEHGIVGNGWYFRDECEVHFWKQAAALVQRPRAWERAKAIDSAFT
                     CANLFWWYAMYATADYSVTPRPMYPADGRKIPDVWTNPPELREWLQRGLGQFPLFRFW
                     GPSTNIAATQWIAGAAIRVDQRCDPTLSLIYLPHLDYCLQRNGPSAEANAKDLRELDA
                     VCGLLFDHFASRDARVIVLSEYGITPVSRPVHLNRVLRESGLLTVRDEMGHELLDAGA
                     SAAFAVADHQVAHVYVNDRSRQNEVRALLEKTPGVARVLDDRGKREMHLDHPRAGDFV
                     ALAGPDSWFTYYYWLDDSRAPDFARTVDIHRKPGYDPAELFIDPKLPAPKAKIALALL
                     KRKLGFRALMEVTPLDATLVRGSHGLPPLNPNDGPLLMTNAAHLLVGSQLNAIDVAEM
                     IQRHVRGE"
BASE COUNT          470 a          790 c          768 g          444 t
ORIGIN      
        1 cactgcggtc ttacatcccg ctaaaacgat tgcactaagc gatcattcag aattcgtgaa
       61 cgataattct tcaggtttgg ctgcgatcct ttgagcggca gaggcggagg tagttaatct
      121 ttgactcatc cgcggttagg caggatgggc ggcacagtat aatttttttt ctgttatatc
      181 gtttgccctt cgcgttgcgg gcagcggtct actactggcg tgccagcgcg acctgtacca
      241 atcagcgagc agttgccgat ctatttccta caggccgtgt ccaattcagg cttcaattat
      301 tctcatcatg tgatggaaac cgctgcgcct gctaaagcat gggccggaac gacgcgatga
      361 agtgcttgag gagtcgtatg cacaagaagt ccgccgttgt cgagagtgat gctaccgccg
      421 ccaccgccgt ggagagcgaa gcccgcgggc agggtccgga ccgccgggcc agccgggtcg
      481 atcgccgcac gggcatcgag cgccggcagc tgtcggccga ggcgtcgaat tacaccggcg
      541 ccgagcgccg cagcggcacg gaccgccgcg acgacacggg cctggaacgc cgccgcgggc
      601 caggccgccg gcggtcggat gaacgccgca gcgcggaaga gggcgagatg aataccgagc
      661 aattcgaatt cgtgatggcc atcgagacct acaagaaggt caaccgcaag atgtacccga
      721 cgtggacgga aatcctggag gtcgtccgcc agctgggcta ccgcaaggtc gagcaccgcg
      781 acatccagtt ggaaaacgtg ccggaaccgg acctgcagaa ggtggcgtag aagtgaatgc
      841 gtgaatgcgt gaatacgtga atacgtgatc actcaccacg gacatgccgc tggatcatct
      901 ctgcaacatc tattgcattg agctgacttc caactaatag gtgggccgcg ttcgtcatca
      961 gcagcgggcc gtcgtttgga ttgagcggcg gcagcccgtg tgatcctcgg accagcgtcg
     1021 cgtcaagcgg cgtcacttcc atcagcgcgc gaaatccgag cttacgtttc agcagcgcca
     1081 gcgcgatctt tgctttcggg gcgggcagct tcgggtcgat gaacagctcc gccgggtcgt
     1141 agccgggctt gcggtggatg tccaccgtgc gggcgaagtc gggggctcgc gaatcgtcca
     1201 gccaatagta ataggtgaac cagctgtcag ggccggccaa ggcgacgaag tcgccggccc
     1261 gcgggtggtc caggtgcatc tcgcgcttgc ccctatcgtc cagcacgcgc gcgacgccag
     1321 gcgttttttc cagcagcgcg cgcacttcgt tctgccgcga ccgatcattt acgtaaacgt
     1381 gagcgacttg atgatccgcc accgcgaacg cagcgctggc gccggcgtcg agcagctcat
     1441 ggcccatctc atcgcgcacg gtcaataggc cactttcgcg cagcacgcgg ttcaaatgca
     1501 ccggccgtga caccggcgtg atgccgtatt ccgacagcac gatcacgcgc gcatcgcgcg
     1561 atgcgaaatg gtcgaacaac agcccgcaca ccgcgtcgag ctcgcgcagg tccttcgcgt
     1621 tcgcctcagc gctcggccca ttccgctgca ggcaataatc caagtgcggc agatagatca
     1681 gcgacagcgt gggatcgcac cgctggtcca cgcggatcgc ggcgcccgcg atccattgcg
     1741 tcgcggcgat gttcgtcgac ggcccccaga atcgaaatag cgggaactgc cccagcccgc
     1801 gctgcagcca ctcgcgcagc tccggcgggt tcgtccagac gtccggaatt ttccgcccgt
     1861 ccgccgggta catcggccgc ggcgtcaccg agtaatccgc cgtcgcgtac atcgcgtacc
     1921 accaaaacag gttcgcgcac gtgaacgccg aatcgatcgc tttggcgcgc tcccacgcgc
     1981 gcggccgctg caccaacgcc gccgcctgct tccagaaatg cacttcgcat tcatcgcgaa
     2041 agtaccaccc gttgccaacg atgccatgct cattgggcca agcgccggtg agatacgtgg
     2101 actgcacgct cgtcgtcacc gcaggaatga ccgcgccaac gctcgccagc cgcccgcgct
     2161 cccgaaaccc cgccaaccgc ggcgtgtgcg gcccgatcag gttcggcgtg aggccgacgg
     2221 cgttgatgac gacggttttg tgcacgtgac gacgtgaata tgtagcacgg gcgtctcgcc
     2281 cgtgtcgagc gacgccaggc acgggctctg gcgcccgtgc tacgaaggtg tcctgcctgg
     2341 tcgttcttcg gatgcgatct cagacgcgcg cacgttttgc cgagtcggca cgatgaccct
     2401 gtgccacgcc aggctcggag cgtactcggg acgacccgga ttcttcggcg ctcaaaagca
     2461 tccgggtcgt cg
//
LOCUS       DATIMH010000023         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5201785, whole genome shotgun sequence.
ACCESSION   DATIMH010000023 DATIMH010000000
VERSION     DATIMH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5201785"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="VLI90_00390"
     CDS             <1..1162
                     /locus_tag="VLI90_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12686.1"
                     /translation="DTAIFDHSADFDPAGDFEAFLRATPAKWVVYLLADADDRPVQLL
                     CVKNLRYSLKRRLGGDEMIGPTKRVNYRDLVRRIHWRRVDSAFEADWLYYEAARALFP
                     QSYQGMVGFRPAWFVQVNPETNFPRYTKTIDLTGKTGVFLGPVEDKHAAGKLIQLVED
                     AFDLCRYYNILTEAPRGLPCAYKEMGKCPAPCDGSISMQQYRQVVEWSTRVLVDPADY
                     VREQQRRMQQAAAELRFETAGKIKAYIDQLSQLGKGPFRHAARLADFQYLSFQRGPRA
                     GTAKLFLITPGQIEQLLCVIDDSFSPGETLRVALTRAAADVAAAPITQEGVERIGIVA
                     HHLFCAKSAHGVFLPMAKIDEKSIAKAWRDLQKQKVPEEVDAEGVVKELQAL"
     gene            1650..2648
                     /locus_tag="VLI90_00395"
     CDS             1650..2648
                     /locus_tag="VLI90_00395"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HSV12687.1"
                     /translation="MPCCQDTGQKRLPSLNMIDRRASSSSASPRKATPSVTSSQQPEI
                     RSLLLYLASERGLADNSLHAYRRDLEDINVEFTQRGVDLKTATADDYREYLRSQSRKG
                     QSTKTIARRVAAIRIFLRFLATQGLDTLPILAHLERPKPERDLPQVLSRAQVAQLIAA
                     PDPASPLFARDVAILELLYASGLRASELCDVKLRDVNLQVCCVRVLGKGMKERIVPFG
                     RAAQQAIERYLLDCRLTLDKRSSDRLFLSRTGKSMERVGLWMLVERYARKSGLLKTVG
                     PHVLRHCFATHLVGGGADLRVVQELLGHSDISTTEIYTHVDQQRLKSIHEKFHPRR"
BASE COUNT          544 a          919 c          858 g          473 t
ORIGIN      
        1 cgacacggcc atcttcgacc actccgccga cttcgacccg gccggcgatt tcgaggcatt
       61 tttacgcgcg acgccggcga aatgggtcgt ctatctgctg gccgacgccg acgaccggcc
      121 ggtgcagttg ctctgcgtga agaacctgcg ctacagcctg aagcgccgcc tcggtggcga
      181 tgagatgatc gggccgacca agcgcgtgaa ctaccgcgac ctggtccgcc gcatccattg
      241 gcggcgggtc gacagcgcct tcgaggccga ctggctttac tacgaggcgg cccgcgcgct
      301 ctttccgcaa agctaccagg gcatggtcgg ttttcgcccc gcgtggttcg tgcaggtgaa
      361 cccggagacg aattttccgc gctacacgaa aacgatcgac ctgaccggca aaaccggcgt
      421 ttttctaggc cccgtcgaag ataagcacgc cgcggggaag ttgatccagc tggtcgaaga
      481 cgccttcgac ttgtgccgct actacaacat cctcaccgaa gccccgcgcg gcttgccgtg
      541 cgcgtacaag gagatgggca agtgccccgc gccctgcgac ggctcgatct cgatgcagca
      601 gtatcggcaa gtcgtcgagt ggagcacgcg cgtgctggtc gatcccgccg actacgtgcg
      661 cgagcagcaa cgccgaatgc agcaggccgc cgccgagctg cgcttcgaga cggcggggaa
      721 gatcaaggcg tacatcgatc agctgtcgca gctgggcaag ggaccgttcc gccacgcggc
      781 gcgattggcg gattttcaat atctctcgtt ccagcgcggc ccccgcgccg gcacggcaaa
      841 actgttcctg atcacgccgg gccagatcga gcagcttttg tgcgtgatcg acgactcgtt
      901 cagccccggc gagaccctgc gcgtcgcgct gacgcgcgcc gccgccgacg tcgccgccgc
      961 gccgatcacg caggaggggg tcgagcgcat cgggatcgtc gcgcatcacc tgttctgtgc
     1021 caaaagcgcg cacggcgttt tcctgccgat ggcgaagatc gacgagaagt cgatcgccaa
     1081 agcctggcgc gacctgcaga agcagaaggt gccggaagag gtggatgccg aaggcgtggt
     1141 gaaggagctg caggcgctgt agatgtcttg ccccctctcc cacgtactgg ctaagaaccc
     1201 gacacttttg caggaacggt caaaaaccga ccacgccggg ttcggagcgt actcgcgacg
     1261 aagcgagatt ctgcggcgct cgaagagatc cgggtcgtcg gagtacctcc gaacccggcg
     1321 tggattggtt tcaagaccgc ttgtatgaaa gtgttgggtc gtcagcacat actcggggga
     1381 gacgggatca gagttaacgt ttcccgccgc ctcacgccga taaagattat cggcacccct
     1441 gccagtgagg cggaatatgt catcaccgac agcagcagca aagcagcgcg tccaccactg
     1501 cccgtttctg aaccgagccg acagccgttg ttccaatcac ttcagcctgg accggctcaa
     1561 ccacgcgttc gagcattgct tcggccgata taaaagttgt ccgacgtatt tggaactgct
     1621 ggtcgaacgc cgcgtgcgcc gcgcgggtga tgccgtgctg ccaggacacg ggccagaagc
     1681 gcctgccgag cctgaacatg atcgatcgcc gcgcctcgtc gagctccgcc tccccgcgca
     1741 aagcaacccc gagcgttacc agcagtcagc agcctgagat ccgctcgctg ctgctatacc
     1801 tggccagcga gcgcgggctg gccgacaatt cgctgcacgc ctatcgccgg gacctggaag
     1861 acatcaacgt cgaattcacg cagcgcggcg tggacctgaa aaccgccacc gccgacgact
     1921 accgcgaata tctccgcagc caatcgcgca aggggcaatc gaccaagacg atcgcccgcc
     1981 gggttgccgc catccgcatc tttctgcgct tcctcgcgac gcaggggttg gacacgttgc
     2041 cgatcctggc gcacctggag cgtcccaagc ccgagcggga tttgccgcag gtgctgagcc
     2101 gcgcgcaggt cgcgcagctg attgccgcgc ccgatccggc gtccccgctg ttcgcgcgcg
     2161 acgtcgccat tttggaattg ctctacgcca gcggcctgcg cgccagcgaa ctatgcgacg
     2221 tgaagctgcg cgatgtgaac ctgcaagtct gctgcgtgcg cgtgctgggc aaagggatga
     2281 aagaacgcat cgtccccttt ggccgcgcgg cgcagcaagc gatcgagcga tatctgttgg
     2341 attgccgtct gacgctcgac aagcgcagca gcgaccgcct gttcctctcg cgcacgggta
     2401 aatcaatgga gcgcgtcggc ctgtggatgc tcgtcgaacg ctacgccaga aagagcggcc
     2461 tgctcaaaac ggtcggcccg cacgtgctac gccactgctt cgccacccac ctcgtcggcg
     2521 gcggagccga cctgcgcgtc gtccaggaac tgctcggcca cagcgacatc agcacgaccg
     2581 agatctacac gcacgtcgat cagcaacggc tcaaatcgat ccacgaaaag ttccacccgc
     2641 gaaggtgaaa taatccctgt catcctcagc gaagcacgta ctcgtgcgca gtcgaacgat
     2701 ctggccgaga agtcgaagaa tcgtcgtttg cgcaaatcag ccgcttccgg cttcgcagat
     2761 caggagccgc tgaattgcga agccgcaagc ggct
//
LOCUS       DATIMH010000024         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1736897, whole genome shotgun sequence.
ACCESSION   DATIMH010000024 DATIMH010000000
VERSION     DATIMH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736897"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VLI90_00400"
     CDS             complement(<1..674)
                     /locus_tag="VLI90_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12688.1"
                     /translation="MTTITSWHINWGDGGSSDVTIPTGSSPLVSTPPHTYAVASVLGY
                     AVAITATGKNAGGSTVQLDMPHLELPLATAPFLPDAIYQGPVPEPAYFSEFDTFAPPV
                     RAGSSGASGTPSIDEFTRSAGPNQSIAVTAESLTTQASPSANDDTSFLTYGQTTVADG
                     VLAPARIQTIDDQKTSLTLPASLTPGSIYLLWPHNSLGYGNPVVVNQPELWWADTSTA
                     YSGQRMS"
     gene            complement(932..1168)
                     /locus_tag="VLI90_00405"
     CDS             complement(932..1168)
                     /locus_tag="VLI90_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12689.1"
                     /translation="MRVFGDVLAAFGWLGLICFCVVLLMFLLTPYDHHPPAPSDIADC
                     FVFALVCFIWSALWRMIGSLAIAVRDIARNSFGR"
     gene            complement(1592..2482)
                     /locus_tag="VLI90_00410"
     CDS             complement(1592..2482)
                     /locus_tag="VLI90_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12690.1"
                     /translation="MLEQRQLLTTVLLTGTPGGDFFQISSDDNDLTYRVTQPNQPDQV
                     YPASYPLNVSITGGGGGDQLYVASGVDVAVLNDPGADGTSLSIDEMDSGTSLTFAAQD
                     TRLNDLSLDPGAAAVVARDGTHLLEVNSFAVGPDDAPANSQLDLSDNGFIIHAGSSDG
                     AAYWRTALQYEVSQGHLNGWAGDGIGSDLLPPDELPHSVLAMAIGNGGNVAANGGAAI
                     NSFFGRPAGPDDLLANVHLSRRCEPRRAGDRRRLPDRPGQRRHRQPDLGRRRRELRRR
                     RGFDGLGFDCGRAKHYAAGA"
     gene            complement(2766..2975)
                     /locus_tag="VLI90_00415"
     CDS             complement(2766..2975)
                     /locus_tag="VLI90_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12691.1"
                     /translation="MICYPQFIKSGWQIGSGPTESQCKQVPRRVKGRGKRWDADNAQA
                     LMALEAIRQSNLEDAYWKICAGNLN"
     gene            3282..3695
                     /locus_tag="VLI90_00420"
     CDS             3282..3695
                     /locus_tag="VLI90_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSV12692.1"
                     /translation="MQLSEHAQWIVNQMLEQVLELDQRVAAAEQRLRQATAADVTVDR
                     LMDQEGVGEVTAWVLRAFVGRFDRFASGKQLSRYCGLSPKNLSSGAKQGDGGLIKASN
                     KILRATAIQGPSLRMSRTTPMGRPRWWPSSNRREI"
     gene            complement(3758..>4051)
                     /locus_tag="VLI90_00425"
     CDS             complement(3758..>4051)
                     /locus_tag="VLI90_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12693.1"
                     /translation="EMNAALEAAMAGFIGVNVTNDPDESRAGMAAITPLAGGALPGDR
                     PKTAADEEVEVKEFGGEFYPVVRSVHKEHKNEEEAATAAVVEPPPPSPPAKKK"
BASE COUNT          716 a         1231 c         1309 g          795 t
ORIGIN      
        1 gacatgcgct gccctgaata ggcggtgctg gtgtcggccc accatagttc cggctgattg
       61 accacgacgg gattgccata accaagcgag ttgtgcggcc aaagcaggta aattgaaccc
      121 ggcgtcagcg aagcgggcaa cgtcagcgac gttttctgat cgtcgatggt ttggatccgc
      181 gccggcgcga gcacgccatc ggctacagtg gtctggccgt aggtcagaaa gctggtgtca
      241 tcatttgcag aagggcttgc ctgagtggtg aggctctcgg cggtgaccgc aatgctctgg
      301 ttgggaccgg cgctgcgggt gaactcgtcg atgctcggcg tccctgaagc gcctgaactg
      361 cccgcgcgga cgggtggggc gaacgtatca aattcactga aatacgccgg ctccggcact
      421 ggaccctggt agatcgcatc cggcaggaac ggcgccgtcg ccagcggcaa ctccaggtgc
      481 ggcatgtcca gctgcaccgt actcccgccg gcgttcttgc cggttgcggt gatcgccacc
      541 gcgtaaccca gcaccgacgc gactgcgtag gtatgcggcg gcgtgctcac cagcggtgaa
      601 gagccagtcg gaatcgtgac gtcgctgctg ccgccgtcgc cccagttgat gtgccaactg
      661 gtgatggtcg tcatcgtgtg cctggcgatc gaccttcccg tctttcatgg gcgggttgtt
      721 ggcgagttgc tgtgccaaat cagaatccgt agacaacaca tacgcaatag ctaaggcggt
      781 ggcggcgaga tcattcgagg ggtgaatgat cgcggcggtt gtaaccccca tgatcgttcc
      841 cttacgcgac gtgtggtaag atgtgaatgt cggcgtcgcc caacgacccg gcattcgccc
      901 ccgccggtcg caacggcagg ggcgttgtta ttcaacgacc aaagctgttg cgcgctatgt
      961 cccgcacggc gatcgccagc gatccgatca tccgccacag agcggaccag atgaaacaca
     1021 ccagcgcgaa tacaaaacag tcagcgatat cactgggcgc gggcgggtga tggtcgtatg
     1081 gggtgagcag gaacatcagc agcacgacac agaagcagat cagccccagc cagccaaacg
     1141 cggcgagcac gtcgccgaaa acccgcatca ccttagcgcc gcgcacgata tctgaatact
     1201 ccggcggctg gtccgcggcg gctgggacgg catcgctttg atcgttggtg ttgtcaatcg
     1261 aacccgtgct atcgatttgt cggacttttg agacaagtat gccccgcgat tgagcatctg
     1321 cgattgcagc ctcgcgagtt ggcgcgatta tcgcgatgtc cgtctctttg cccgactcac
     1381 gcatcgcccc gtcaatcagc caagtaccca tagcccttcc cgtcggagat gttcacgaaa
     1441 gcgtagcgta cccatgagcc aagaagctga cataagtgtc tacacgaacc tgttgctccg
     1501 caaggacgag ctggtcgctg tcgtgttcgc ggtcagctgg ttgcctggca gcgtgaacga
     1561 atacggctgt ccctgtgtca ccggccccag gtcaggcacc tgcggcgtaa tgttttgctc
     1621 gcccgcaatc aaacccaagt ccgtcgaatc cacgccgccg tcgtagttca cgtctccgtc
     1681 gacccaagtc gggctgccga tgccgacgtt gccctgggcg atctggtagt cggcggcggt
     1741 cacctgcccg tcgaggttca catcgccttg ataggtgtac attcgccagc aggtcgtcgg
     1801 gaccggccgg acgcccgaag aagctgttga tggcggcccc accgttggcg gcgacgttgc
     1861 caccgttgcc gatggccatc gccagcacgc tgtgcggcag ctcgtcgggc ggcaataaat
     1921 cgctgccgat gccgtcgccg gcccagccgt tgagatggcc ctggctcact tcgtactgta
     1981 gggccgtgcg ccaataggcc gccccgtcgc tgctgccggc gtggatgatg aagccgttgt
     2041 cgcttagatc caactggctg ttggccgggg catcatccgg gccaacggca aagctgttaa
     2101 cttcgagcaa gtgggtgccg tcgcgggcaa ccacggcggc agcgccggga tcgaggctca
     2161 ggtcgttcaa ccgcgtgtcc tgcgcggcaa aggtcaagct cgtgccgctg tccatctcgt
     2221 cgatagatag gctggtgccg tcggcgccgg ggtcgttgag cacggcgacg tcgacaccgc
     2281 tggccacata gagttggtcg ccaccaccgc cgccggtgat gctgacgttc agtggatagc
     2341 tggcgggata gacttggtcg ggctggtttg gttgggtgac gcgataggtc aaatcattgt
     2401 cgtcgctgct gatttgaaag aagtccccgc cgggcgttcc ggttaagagg acagtcgtca
     2461 gcagttgacg ttgttcgagc atctacacct cgcaggcacg caatcgctcc gggcttgctc
     2521 caccgtgcgt gaatttgaca ttgctccaaa tttgcgcagc catggctgcc gcggcacgtt
     2581 ggatcgtacc aatggctcgc atgatacctt cccttttgca gaaatcgccg agaaatgtgg
     2641 gcccgccctc tgcttgcgcc cccgagaact gcgctgctgt gtagatttgc cccccgctgg
     2701 tgtcaagcaa aatctgaaac aagtcaacaa agtgacgatg ggtctgcccg tttttccggg
     2761 agaagctaat tcagattgcc ggcgcagatt ttccagtagg catcctccaa attgctctgc
     2821 cggatcgcct ccagcgccat cagggcttgg gcgttgtcgg cgtcccagcg tttgccgcgg
     2881 cccttgacgc gccggggcac ctgtttgcac tggctctcgg tgggaccaga gccgatctgc
     2941 cagccgctct tgatgaactg tggatagcag atcatctccc ggcggtcggt gatgtagtta
     3001 atcagcagtt gggcggcgtg acgtttgctt gatcgcggag ctgacgcggg tgggatacct
     3061 gccgcgcacc tggcttccgc ggctcgactg agctcgccga agtccggcgt acatccggca
     3121 gctgcggcag ctggtgggct atcgccagca gttggtcgac caacggcggg cgctgaagct
     3181 gcgggttggg gcgctgctgc gcgagcagcg ggtggtggtg ccccaaggat ccgcaacgct
     3241 cgcgctggag ccgggcgtgg atcagcttcg cacgctgcac gatgcagctc agcgagcatg
     3301 cgcagtggat cgtgaaccag atgctggagc aggtgctgga gctggaccag cgggtggcgg
     3361 cggccgagca acggctgcgg caggcgacgg ccgctgacgt gacggtcgat cggctgatgg
     3421 accaggaagg cgtgggtgaa gtgacggcct gggtgctgcg ggcgttcgtc ggccgcttcg
     3481 atcgctttgc cagcggcaag cagttgtcgc gttactgcgg gctctcgccc aagaacctct
     3541 ccagcggtgc caagcagggc gacggcggct tgatcaaggc gtcgaacaag atcctgcggg
     3601 cgacggcgat ccaggggccg tcgttacgga tgtctcggac aacccccatg ggcaggccgc
     3661 gatggtggcc gagctcaaat agaagagaaa tttaaaatca ccctcacccc agccctctcc
     3721 cggagtaccg ggagaggggg aaagagccgt cgaaatgtca cttcttcttc gccggtgggc
     3781 taggcggtgg cggctcgacg accgccgccg tcgcggcttc ttcctcgttt ttgtgttcct
     3841 tgtgcacgct gcggacgacc ggataaaact ccccgccaaa ttctttgact tcgacttcct
     3901 cgtccgccgc cgtcttgggg cgatcacccg gcagcgcgcc accagccagc ggggtgatcg
     3961 cggccatgcc ggcccggctt tcgtcggggt cgttggtcac gttgaccccg atgaacccgg
     4021 ccatcgcggc ctccagcgcc gcgttcatct c
//
LOCUS       DATIMH010000025         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_870791, whole genome shotgun sequence.
ACCESSION   DATIMH010000025 DATIMH010000000
VERSION     DATIMH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870791"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VLI90_00430"
     CDS             complement(<1..425)
                     /locus_tag="VLI90_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HSV12694.1"
                     /translation="MASPSPSTTATQLNNEQINRYKRHLILPEVGTEGQLKLLNARVL
                     CIGAGGLGCPISLYLAAAGVGTIGLADIDVVSPSNLQRQILFGVSDVGEDKVKAAANR
                     LKDINPDVKIIEHKTIVNSSNVLDLVAAYDVIIDGTDNFP"
     gene            complement(607..1128)
                     /locus_tag="VLI90_00435"
     CDS             complement(607..1128)
                     /locus_tag="VLI90_00435"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSV12695.1"
                     /translation="MALFPDDFPFGSRTALAEPVSSITSVEPLQAVEPSAPVAQVIEP
                     VAPVIAVAITRAAPPPAHERRKALRHTLVARAQLRADMGFTGLVNVDVQNISLIGVRF
                     LSQHPIDAHQKAQLRVEAGPLRWSSRVRVITCQADNAGRYQIGCQFVGNEMMKPWGAT
                     GASGNAAPAAAAA"
     gene            complement(1292..2068)
                     /locus_tag="VLI90_00440"
     CDS             complement(1292..2068)
                     /locus_tag="VLI90_00440"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV12696.1"
                     /translation="MPRYRAIAEYYDAEYADSKMLQQDVPFFLGHLPARRRQAVLELC
                     VGTARAAIPIAQAGHHVIGLDYARDLLAIARRKRDAVGLADDQLQLRFGDVLNIRLQQ
                     SFDWVCLFFNTLLAFPKLAEQDRLLQRVRQHLKPRGRFWLDVFQPDLNLLAGTRTRGL
                     DPHVFYVPALDRTVHQTTEIRRDPAQQVQHVTFHYTWFDDRGRKHQERNRFTLTWMFP
                     RELQMLLERNGLRIEHLYGNYDGSKVTADSPRMIACCARA"
BASE COUNT          392 a          660 c          710 g          390 t
ORIGIN      
        1 gggaaattgt cggtgccgtc gatgatcacg tcatacgcgg cgacgaggtc gagcacgttg
       61 gagctgttca cgatcgtctt gtgctcgatg atcttcacgt ccgggttgat gtccttgagt
      121 cgattggcgg cggccttcac cttgtcctcg cccacgtcgc tgacaccgaa caggatttgc
      181 cgctgcaggt tggacggcga cacgacgtca atatcggcca gtccgatcgt gcccaccccc
      241 gcggcggcga gatacaggct gatcgggcaa ccgagcccac cggcgccgat gcacagcacg
      301 cgggcgttca gcagtttcag ctgaccttcg gtgccgactt ccggcaggat caggtgccgc
      361 ttgtagcggt tgatctgttc gttgttcagt tgagtcgcgg tcgttgaagg ggatggagat
      421 gccatggcgt agaccttaaa tgaaaaaacc atgccgaagt gcggcatggc ccgacgctga
      481 ggagaacatc tgctgcgctt tagcgagttt cgggccgcaa gctgcgaatt caaatcgccc
      541 ggaacgcgag ctgccgcgtc cctctttaat tgttcaagga aagtctagga atcagcctgg
      601 ggcgggtcaa gcggcggcgg ccgcgggggc ggcgttaccg ctggcgccgg tcgcgcccca
      661 cggcttcatc atctcgttgc cgacgaactg gcagccgatc tgataccgcc cggcgttgtc
      721 tgcctggcag gtgatgacgc gcacgcgcga cgaccagcga agcggccccg cttccacgcg
      781 aagctgggcc ttctgatgcg cgtcaatggg atgctgcgac aggaagcgca cgccaatcaa
      841 tgaaatgttc tgcacatcga cattaacgag gccggtgaac cccatgtccg cgcgaagctg
      901 agcccgcgcg accagcgtgt ggcgaagggc cttgcggcgc tcatgcgccg gcggcggtgc
      961 cgcgcgcgtg atcgcgactg cgatcactgg cgcaacgggt tcgataactt gcgccactgg
     1021 cgccgacggc tcaaccgcct gcagcggttc gacagacgtg atcgaagaca ccggctcggc
     1081 caatgcggtt cggctgccga atgggaagtc gtccggaaac agagccatgt cggtcgtatc
     1141 ggatattccg gggggaggct ttatcgaggg cgcgccgcaa gcgatcgaat gacgctccgt
     1201 gatagccgca ggcgacagcc agcgggggtc taagaatatc agcccggttg atggtccagc
     1261 gcccccgctg gctgtcgcct gcggctgtca gctacgcccg cgcgcagcac gcgatcatgc
     1321 gcggggaatc tgcggtgacc ttgctgccgt cgtagttccc gtacaggtgc tcgatccgca
     1381 gcccgttgcg ttccagcagc atctgaagct cgcgcgggaa catccacgtc agcgtgaagc
     1441 gatttcgctc ctgatgcttt cggccgcggt cgtcgaacca tgtgtaatga aacgtgacat
     1501 gctggacctg ctgggcgggg tctcgacgaa tctccgtcgt ctgatgcacc gtgcggtcca
     1561 gagccggcac gtaaaacacg tgcgggtcca agccgcgcgt gcgcgtgccg gccaataaat
     1621 tcaggtccgg ctgaaagacg tcgagccaga atcgtccgcg cggtttcaga tgttgccgga
     1681 cccgctgcaa cagccggtcc tgctcggcca gcttgggaaa cgccagcagc gtgttgaaga
     1741 acagacagac ccaatcgaac gactgctgga gtcgaatgtt caggacgtcg ccgaagcgaa
     1801 gctggagttg atcgtccgcc agtcccaccg cgtcgcgctt tcggcgggcg atggcgagca
     1861 ggtcgcgcgc gtagtccaaa ccgatcacgt ggtggccggc ctgtgcgatg ggaatcgccg
     1921 cccgcgcggt gccgacgcac agctccagca ccgcctgtcg ccgccgggcc ggcaggtggc
     1981 ccaggaagaa cggcacgtcc tgctgcagca tcttcgagtc ggcgtactcg gcgtcgtaat
     2041 actcggcgat ggcgcgatag cgtggcatgc gtaatgactt tgatagccgg gggcggaagc
     2101 cccggtcttt cacgacagca cagaccgggg cttacgcccc cggctatcaa gt
//
LOCUS       DATIMH010000026         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_582162, whole genome shotgun sequence.
ACCESSION   DATIMH010000026 DATIMH010000000
VERSION     DATIMH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582162"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VLI90_00445"
     CDS             complement(<1..741)
                     /locus_tag="VLI90_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HSV12697.1"
                     /translation="MFDFVRKHTRLMQFLLFLLIFPSFVLFGVERYNRFKDQGEAVAK
                     IDGREILQNEWDTAHKQEVERLRQQMPTLDPKLLDSPAARYATLERIVRDRVLSAAAA
                     QSKLVTGDQRLARELQRNEMIASLRGPDGKLDMARYRQLVGQQGMSPEMFEANVRADI
                     SSRQVLAGLGGTAFATPAQARLALDAFFEKRVVQVARFAAADYAKQVNPSDAELEAYY
                     KSNTAQFQAPEQADIEYVMLDLDSVKKNV"
     gene            complement(807..882)
                     /locus_tag="VLI90_00450"
     tRNA            complement(807..882)
                     /locus_tag="VLI90_00450"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(847..849),aa:Val,seq:tac)
     gene            complement(900..1172)
                     /locus_tag="VLI90_00455"
     CDS             complement(900..1172)
                     /locus_tag="VLI90_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSV12698.1"
                     /translation="MNKTELIEHIAKHADISKAAATRALESMIGAVKTTLKKGGSVSL
                     VGFGTFAVGKRAARSGRNPRTGAAIKIKAAKVPKFRPGKALKDALN"
     gene            complement(1457..2091)
                     /locus_tag="VLI90_00460"
                     /pseudo
     CDS             complement(1457..2091)
                     /locus_tag="VLI90_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
BASE COUNT          387 a          649 c          639 g          432 t
ORIGIN      
        1 gacgttcttc ttgaccgagt ccaggtccag catcacgtat tcgatatcgg cttgctccgg
       61 cgcctggaac tgcgctgtgt tgctcttgta gtaggcctcc agttcggcgt cgctcggatt
      121 cacctgcttg gcgtaatccg cggcggcgaa gcgcgccacc tggactacgc gtttctcgaa
      181 gaaagcatcc agcgcgaggc gcgcctgcgc cggcgtggcg aacgcggtgc cgccaaggcc
      241 ggccaggact tgccgcgagg aaatgtcggc tcggacgttg gcttcgaaca tctcgggact
      301 catcccctgc tgtcccacca actggcgata gcgagccatg tccagtttgc cgtcggggcc
      361 gcgtagcgaa gcgatcattt cattgcgctg cagttcgcgc gccaggcgct ggtcgccggt
      421 gacgagcttg gattgcgccg cggcggccga gagcacgcga tcgcgcacga tccgctccag
      481 cgtcgcgtac cgcgccgccg gcgaatccag cagcttggga tcgagcgtcg gcatctgctg
      541 gcgcaagcgc tccacttcct gtttgtgcgc ggtatcccat tcgttttgca gaatctcgcg
      601 cccgtcgatc ttggccacgg cttcgccctg gtccttgaag cggttgtagc gctcgacccc
      661 gaacagcacg aacgacggga agatcagcag gaacagcaga aactgcatga gccgcgtgtg
      721 cttgcgaaca aagtcaaaca tgatgttcaa aaacccgata ggactgacaa aaacaaaagg
      781 cgaacaactg ttcgccttcg catgtttggt gggtgctgac gggatcgaac cgccgaccta
      841 cgccttgtaa gggcgccgct ctaccagctg agctaagcac ccccaccgaa acggccgtct
      901 cagttcagcg catccttgag cgccttgccg ggacggaact tgggaacttt ggcagccttg
      961 attttaatcg ctgcaccggt gcgtggattg cgcccgctcc gggcggccct cttgcccacg
     1021 gcgaaagtac cgaagcccac cagcgagacc gagccgccct tcttcaaggt cgtcttcacc
     1081 gcgccgatca tcgactccag cgcgcgggtg gccgccgcct tggagatatc cgcatgcttc
     1141 gcgatgtgct caatcagttc tgttttgttc acgggaaccc ctcgtagaga gaaagaattg
     1201 acaggaatgt cctctggtat tttttgtcct ccctgtcccg cggcgacgtt ttcgtctttc
     1261 agcagggtga cgggagtctt tttttgcggc gtacagccac tgatgaatta gagccacgca
     1321 cccaaagcgt gtcaatacgc ggggcgggtt tacaacagca tcaaagcgga acttgacgct
     1381 tccttgatcc acgcaggttg cgcgaagttc cgaattcgac gctgcccatt ttttcatggg
     1441 cagaaacacg gcaaagctac agcgccgccg cgatcgcccg gccgacgtcg ctggtggcgg
     1501 cgctgccccc gatgtcgggg gtgcgtggcg cgccgctctg cggattgaga accttttcga
     1561 ttgcggcgag gatcgcgtcg tgcgcctcgc gatagcccag gaattcgagc atcattgcac
     1621 cgcaccatat ctggccaatc gggttcgcaa tgttgcgacc gaagatatcg ggcgccgatc
     1681 cgtgtaccgg ctcgaaaagc gacgggaact tgcgatccgg attgaggttc gcgcttggcg
     1741 cgataccgat cgtgccggtg caggccggtc ccaggtcgct gaggatgtcg ccgaacaggt
     1801 tgctcgccac caccacatcg aagaaatccg gccgctgcac gaaatgcgcg gtgaggatgt
     1861 cgatatgaaa tttgtcgact ttcacctgcg gatactcttc gccatctccg cgacgcgttc
     1921 gtcccaataa ggcatggtga tggcgatgcc gttggatttg gtggcgctgg tcaggtgctt
     1981 cttcgggcgc gagcgtgcga ggtcgaacgc gaacttcaga acgcggtcga caccgacgcg
     2041 ggacatcacc gtttcctgca tgacgatctc gcgctccgtg cccgggtaca tgcggccgcc
     2101 gatgctc
//
LOCUS       DATIMH010000027         6815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4766, whole genome shotgun sequence.
ACCESSION   DATIMH010000027 DATIMH010000000
VERSION     DATIMH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4766"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1487
                     /locus_tag="VLI90_00465"
     CDS             54..1487
                     /locus_tag="VLI90_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV12699.1"
                     /translation="MNVSLTPPEDDLRRPPGEDYPKIGGVEIRAGSGGPHRPPGYAGH
                     AIDPDVDPAAPYAPQVVAREEPDTPLPDTGMALGSQLGDRDVAGLAPGALTTDPNMRD
                     VGLAPGAMTTDANLPPSGGRVEAHPGEVKAGTSPGGAACPDAPPPPLPADARDAKGAR
                     VDGGPESVPRGRATPHMDRSQLQAESPQVQNEPKGPFPPQHQGKPGLESELNPRPQFA
                     APLYKPAGKLLDKVALITGGDSGIGRAVATLYAREGADVAVVFLPAELTDAQETQRIV
                     QHFGRRCLLIPGDLTESKFCDAAIEQTVREFGKLDILVSNAGYQNRVPSIEQLSDDEI
                     EETFRTNIFAYIRLCRAAVKHLKPGGAIIATSSETGLEGAKQLLAYSATKGAINAFTK
                     TLAMELAEKGIRVNAVAPGPVWTPLNPADAGMTAEKVSKFGSSTLFKRPAQPEEIAPA
                     YVFLASDADSSFITGFVLEEMGKTTAG"
     gene            1689..2687
                     /locus_tag="VLI90_00470"
     CDS             1689..2687
                     /locus_tag="VLI90_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSV12700.1"
                     /translation="MPDVTPPSKGQDLRDNAGQLGEGGDTRRGEIGSRGPRKPDPDTR
                     PTEMPADITNVVRPGAPGTRISDPGEGVVGPDANIGGPTHRPADAKAAAPCGIPHVPP
                     IPEQAAIEMGLQDAIKNGINRVEDDLSIGSPRLSVPSDRVGPENGRTGLPELQDPNFT
                     AASQSRGASRDGKTAPDAGGAGDEPVNRRDPSSIGDLMTADVECCTPQTELNYVARMM
                     ADHNVGAIPVVEHTGAMKPVGIVTDRDIVVRVIAKNQDPYSLNASACMSTDPLTVTPE
                     TGIEDCLRQMEDRKVRRVLVVDGSGRCIGIVAQADIVLNVPRQTAAELVKEVSQPG"
     gene            2878..4011
                     /locus_tag="VLI90_00475"
     CDS             2878..4011
                     /locus_tag="VLI90_00475"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12701.1"
                     /translation="MPSLNELWFTKLLADRQPPCVSIYLPMHRAAPSAKENEISYRDM
                     VEQAKVSMRNRYDDRLIDQMAEKLHGIAGDNSFWVGDRDGLAVFASPDFQQVIELQQP
                     VDPLCMVADTFHIKPLVRILQSADRFQVLCFTLNGVQLYEGDQHRLAPVPLNNVPQSP
                     ADVAGMRLSDATTATADFVQGQKQAKQGDAAADEPVNVQHFMQAVDRAIWENHSRRSR
                     LPLVLIASEKHHDPFRRITRNQYLVDVGVMLDPKAIPIDRIRAEAWQVMEPRFKQNVA
                     KLADQFRAAKAHHKGSDELMEVAQAAADGRVGTLLVRADAQIPGMLSQNSGFIEQARP
                     EDPRADDVLDDLTELVLKRDGQVLVLPPEAMPTDTGVAAIYRY"
     gene            complement(4204..4563)
                     /locus_tag="VLI90_00480"
     CDS             complement(4204..4563)
                     /locus_tag="VLI90_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSV12702.1"
                     /translation="MVLGFAIALLGVLAVQHWLNPTDVTEPQPPHGARYDELADRMDP
                     NVADVGALSALPTLGEKRAKAIIDYREDFVRRHPGKRAFESARDLLKLKGFGAAMVAN
                     LEPYLIFETPTTSPATQ"
     gene            complement(4623..6152)
                     /locus_tag="VLI90_00485"
     CDS             complement(4623..6152)
                     /locus_tag="VLI90_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HSV12703.1"
                     /translation="MSLIFEIYREGKRLTEFVPSGAVAMGPESVPVQADISFEGGLLY
                     VRRPDDHAIGVGLLWDAGIHGAYFMETTRLSERPTPYNLNVELARFRLMRIVQKQEDW
                     NLFDFPRAEKFGTKFREAQEIFADALGKLHEPAAASLLADQALSLALELSEELGAFHA
                     DLLIQRRKANGAFVRHVIGCRVDPTVQNEKYRETLAGNFDYAIVPMTWRQMEPEEHAW
                     DTAPVDELVELLSRKRMPIIAGPIIQLDDRHLPDWMFIWEHDFDSIRELAYEYVQKIV
                     GRYRKAVSMWNVVAGLHTNSAFSLSFEQIIELTRLLVSQVKALLPNTRTLITVTQPFG
                     EYGAKKTPSVPPMLYAEMVAQAGINFEAFGVEIEMGVPAPGRFTRDLFQLSCMLDRFS
                     TLGRPLFVTAVGAPGRNGPDADDKSEGRLDPSHGGRWKRPWDPQLQAEWMEAVYHLAL
                     SKPYVESLAWGDLADISPNLPSGGLLDDVLRPKPAFDKLQEMRTKFHSWHGRKTAAPA
                     T"
BASE COUNT         1288 a         2186 c         2230 g         1111 t
ORIGIN      
        1 ccactagcag acccacccgt tcgcccgatc aaaaatctga ttccgaaaaa gcgttgaacg
       61 tatcgctcac cccgccggag gacgacctgc ggcggccgcc gggcgaggac tatccgaaaa
      121 tcggcggcgt ggaaatccgc gccggcagcg gtggtccgca ccgcccgccg ggatacgcgg
      181 ggcatgcgat cgatcccgat gtcgatccgg ccgcgccgta tgcgccgcag gtggtcgcgc
      241 gtgaagagcc ggacacaccg ctgcccgaca ccggcatggc gctgggcagc cagctgggcg
      301 accgcgacgt tgccggcctg gcaccgggcg cgctgacgac cgatcccaac atgcgcgacg
      361 tcggcctcgc gcccggcgcg atgacgaccg acgccaattt gccccccagt ggcgggcggg
      421 tcgaagcgca ccccggtgaa gtgaaggccg gcaccagccc cggcggggcg gcgtgtcccg
      481 atgcgccacc gccaccgttg ccggccgatg cgcgcgacgc caagggcgcg cgcgtcgatg
      541 gcggacccga atcagttccg cgcgggcgag ccacgccgca catggatcga tcgcagttgc
      601 aggccgaatc gccgcaggtt cagaacgaac cgaaggggcc gttcccgccg cagcatcaag
      661 gcaagcccgg gctggagtcg gagttgaatc cgcgcccgca gttcgccgca ccgctgtaca
      721 agccggcggg caagctgctg gacaaggtcg cgctgattac cggcggtgac agcggtatcg
      781 gccgggccgt cgccacgctg tatgcgcgcg aaggcgcgga cgtcgccgtc gtctttctcc
      841 cggctgaact cacggacgcg caggagacgc agcgcatcgt ccagcatttc ggccggcgct
      901 gcctgctcat cccgggcgac ctgaccgagt cgaagttctg cgatgccgcc atcgagcaga
      961 ccgtgcgcga gttcggcaag ctcgacatcc tcgtctccaa cgccgggtat cagaaccgcg
     1021 tccccagcat cgagcagctg agcgatgacg agatcgagga gacgttccgc acgaacatct
     1081 tcgcgtacat ccggctgtgc cgcgcggcgg tgaagcacct gaaacccggc ggcgcgatca
     1141 tcgcaaccag ttccgagacc ggcctcgaag gcgccaagca attgctggcg tactccgcga
     1201 ccaagggcgc gatcaacgcg ttcaccaaga cgctggcgat ggagctggcg gagaagggga
     1261 ttcgcgtgaa cgcggtggcg ccggggccgg tgtggacgcc gctcaaccct gccgacgcgg
     1321 ggatgacggc ggagaaggtg tcaaagttcg gcagcagcac gctgttcaag cgtccggccc
     1381 aaccggaaga gatcgccccc gcgtacgtct tcctcgccag cgacgccgac agcagcttta
     1441 tcacggggtt cgtgctggaa gagatgggca agacaaccgc ggggtgagac gcgggtttgt
     1501 cttaccccct ctcccggtac gccgggagag ggctggggtg ggggtgattt ggaatatcga
     1561 tgaccgttgg aaatccaaaa tcaccctcgc cctaaccctc tcccggagta ccgcgagagc
     1621 cggaccagag cggcccgcta aacgagcgac gcgatgaacg tagcaacaag agcgaaagcg
     1681 aggaagccat gcctgatgta accccaccgt ccaaaggcca ggacctgcgc gacaacgccg
     1741 ggcagctcgg tgaaggcggc gacacccgcc gcggtgaaat cggctcgcgc ggcccgcgta
     1801 agccggaccc ggatacgcgg ccgacggaga tgccggcgga tatcaccaat gtggttcgtc
     1861 ccggcgcacc gggcacgcgc atcagcgatc ccggtgaagg cgtcgtcggt cccgatgcga
     1921 acatcggtgg cccgacacat cgcccagccg acgccaaagc cgccgcgccc tgtggcatcc
     1981 cgcacgtgcc gccgatcccc gagcaggcgg ccatcgagat gggcctgcag gatgcgatca
     2041 agaatggcat caaccgcgtg gaagacgatc tgtcgatcgg ctcgccgcga ttgagtgtgc
     2101 cgagcgatcg cgttggtccc gaaaacgggc gcacgggact gccggagttg caggacccga
     2161 acttcaccgc cgcctcgcaa tcgcgcggcg caagccggga cggcaagacc gccccggacg
     2221 ccggtggtgc cggcgatgaa cctgtgaacc ggcgcgatcc gtcttcgatc ggtgatctga
     2281 tgaccgccga cgtcgaatgc tgcacgccac agacggagct gaactacgtc gcacggatga
     2341 tggccgacca caacgttggc gcgattccgg tggtcgagca caccggcgcg atgaagccgg
     2401 tcggcatcgt caccgatcgc gacatcgtcg tgcgggtgat cgcgaagaat caggatccat
     2461 actcgctgaa cgccagcgca tgcatgagca ccgatccgtt gacggtcaca ccggagaccg
     2521 gcatcgaaga ttgcctgcgg cagatggagg accgcaaggt ccgccgggtg ctggtggtgg
     2581 acggcagcgg gcgatgcatc ggcatcgtgg cgcaggcgga tatcgtgctg aacgtcccgc
     2641 ggcaaaccgc ggcggagctg gtgaaggaag tgtcgcagcc ggggtgaggg acgattgaca
     2701 gccgagacac gggtacaggg agactaggag acacggggac aaggagacag ggagacacgg
     2761 agcaaaggcc gcgaaatctg cgttttcgta tttctccttg tcgccttgtc tccgtgtctc
     2821 cttgtctctg tggttacaag ttttgtcggg agctttaaac aaacgatgga gagagccatg
     2881 ccctcgttga acgaactgtg gtttacgaaa ctgcttgccg atcgccagcc gccgtgtgtg
     2941 tcgatctatc tgccgatgca tcgcgcggcg ccgtcggcga aggagaacga gatctcgtac
     3001 cgcgacatgg tcgagcaggc gaaggtgtcg atgcgcaatc gctatgacga tcggctgatc
     3061 gaccagatgg cggagaagct gcacggcatc gccggtgaca acagcttctg ggtcggcgac
     3121 cgcgacgggc tggcggtctt cgcgtcgccg gattttcagc aggtgatcga gttgcagcaa
     3181 ccggtcgatc cgctgtgcat ggtagccgac acgtttcaca tcaagccgct cgtgcgcatc
     3241 ctgcagagcg ccgatcgatt ccaggtgttg tgcttcacgc tgaatggcgt gcagctgtac
     3301 gagggcgatc aacatcgcct cgcgccggtg ccgctgaaca atgttcctca aagtccagcg
     3361 gatgtcgcgg ggatgcggtt gtcggatgcg acgacggcca ccgcggattt cgtacagggg
     3421 cagaagcagg ccaagcaagg cgacgcggcc gcggacgagc ccgtgaacgt gcagcatttc
     3481 atgcaggcgg tggaccgggc gatctgggaa aaccattcgc ggcgttcgcg gttgccgctg
     3541 gtgctgatcg catcggagaa gcatcacgac ccattccgcc ggatcacgcg gaaccagtac
     3601 ctcgttgatg tgggcgtgat gctcgatccg aaggcgatcc cgatcgatcg catccgcgcc
     3661 gaggcgtggc aagtgatgga gccgcgcttt aaacaaaacg tggcgaagct ggcggaccaa
     3721 tttcgtgcgg ccaaggcgca ccacaaggga tcggacgagc tgatggaggt cgcccaagcc
     3781 gccgccgacg gccgcgtggg tacgctgctg gtccgcgccg atgcgcagat cccgggcatg
     3841 ctgtcgcaga acagcggctt catcgaacaa gctcggccgg aggatccgcg cgccgatgat
     3901 gtgctggatg acctgaccga gctggtgctg aagcgcgatg gccaagtgct ggtgctaccg
     3961 ccggaggcga tgccgaccga tacgggtgtg gcggcgattt atcgttattg aaattacgct
     4021 gatgaggagc cgcgcctcca cgagtgcagc cgcttgcggt ttattgtctg ttcttcaatc
     4081 gaagagatgc taagcggcca gcggctgacc ctgcgcgagt gaacgttcaa tcacttctcg
     4141 gccagatcct acgactgcgc accggtattt gcttcgctca ggataaacta aaaggctctc
     4201 gcctcactgt gtcgccgggc tcgtcgtcgg cgtctcgaaa atcagatatg gctccaagtt
     4261 cgccaccatc gccgcgccga aacctttcag cttcagcaaa tcccgcgcgc tctcgaacgc
     4321 acgtttacct ggatgccggc gcacaaaatc ttcgcggtag tcgatgatcg ccttggcgcg
     4381 tttctcgccc agcgtgggca gcgcggacag cgcacccaca tccgcgacgt tcgggtccat
     4441 ccgatccgct agctcgtcat accgcgcacc gtgaggcggc tgcggttcgg tgacatctgt
     4501 gggattgagc cagtgttgaa cggcgagcac gccgagcagg gcgatggcga agccgagcac
     4561 cacgcgtcgc tgcgacgccg tccacacgcg tgggaggggc agccgtcgcg gacgcgggcg
     4621 catcacgtcg ccggggcggc ggtcttgcgc ccgtgccagg aatgaaattt cgtgcgcatc
     4681 tcctgcagct tgtcgaacgc cggcttcggt cgcagcacgt cgtcgagcaa ccccccgctc
     4741 ggcaggtttg ggctgatgtc ggccaagtcg ccccaggcca ggctctcgac gtatggcttg
     4801 ctcagcgcca ggtgatagac tgcctccatc cactcggcct gcagctgcgg atcccacggc
     4861 cgcttccatc gcccgccatg actgggatcg agccggcctt ccgacttgtc gtccgcatcg
     4921 ggcccgttgc ggcccggcgc gccaaccgcc gtgacgaaca gcggccggcc cagcgtggag
     4981 aaccgatcga gcatgcaact cagttgaaac aggtcgcgcg tgaatcgccc cggcgccggc
     5041 acgcccatct cgatctcgac gccgaacgct tcaaagttga tccccgcctg cgcgaccatt
     5101 tccgcgtaca acatcggtgg gacgctcggc gttttcttgg cgccgtattc accgaacggt
     5161 tgagtgaccg tgatcagcgt gcgcgtgttg ggcagcagcg ctttcacctg cgacaccagc
     5221 agccgggtca actcgatgat ctgctcgaag ctgagcgaga acgcgctgtt cgtgtgcagc
     5281 cccgcgacga cgttccacat gctgacggct ttgcgatagc gcccgacgat cttctgcacg
     5341 tattcatacg ccaattcgcg aatcgaatcg aaatcgtgtt cccaaatgaa catccagtcc
     5401 ggcagatggc gatcatccag ttggatgatc ggtcccgcga tgatcggcat gcgcttgcgg
     5461 ctgagcagct ccaccaactc gtccaccggc gcggtgtccc acgcgtgctc ttccggctcc
     5521 atctgtcgcc aagtcatggg gacgatcgcg tagtcgaaat tcccggcgag tgtctcgcga
     5581 tatttttcgt tttgaacggt cggatcgacg cggcaaccga tcacgtgccg gacgaatgcc
     5641 ccgttggcct tgcggcgctg gatcaacaaa tcggcgtgaa acgcgccaag ctcttctgac
     5701 agttccaacg ccaaggacaa agcctgatcc gccagcaacg atgccgcggc gggctcgtgc
     5761 agcttgccga gcgcgtcggc gaaaatctcc tgcgcttcgc ggaacttcgt gccgaatttt
     5821 tcggcgcgcg ggaagtcgaa cagattccag tcttcctgct tctgcacgat ccgcatcagg
     5881 cgaaagcggg cgagctcgac gttcaggttg tacggcgtcg gccgctccga cagccgggtc
     5941 gtctccatga aatacgcgcc gtggatgccc gcgtcccaca gcaggccaac gccgatcgcg
     6001 tggtcgtccg gccggcgcac gtacagcagc ccgccttcga acgagatgtc ggcctgcacc
     6061 ggcacgcttt ccggtcccat ggcgaccgcg ccagacggca cgaactcggt gagccgtttc
     6121 ccctcgcgat aaatttcgaa tatgagcgac ataagaaaat cggcgagcgc gcaggcgcgc
     6181 gaatgaaaga acgatacctc cgccagcccg gcatcttagc agagaccccc aaaccctcaa
     6241 atgcgcggga gttccctagc cgcttgcggc ttcgcaatcc agtcggcatg gctcgcaaag
     6301 ccgcaaagtc gcaggagcgc gcagaggcgc gggcgcgcga cgacgaggac gataaaagag
     6361 tgcggcggcc gccggtgagg aacgaccgcc gcagcggagg gtgacaacgg tgagctaaga
     6421 tcaacgttct cgggcgccgg cggcgcgaca gtagcgagac acccccgagc aggaccagac
     6481 cgactgacgc cggttccggg accgacgcgc tgccgccagc agcgacgacg gcgagcggag
     6541 tggccgacag tgcgccgctg ccgaaggacg ggccggtggc caagtgatta ttgtagttgg
     6601 tcgccagcgc gcccaggtcc gcgacgttca cggtgccgtc ctggatgaag tcgccgttgc
     6661 cccagctcat cccgccggtg atgttgtagt tcgtcgccag cgcgcctaag tcgccgacgt
     6721 tcacgatgcc gtcgaggttc gtgtcgccct tgagcgtgta gcgcacttcc accttgccgg
     6781 cgtccgcgcc gctcaggtcg atgtagccaa gcgct
//
LOCUS       DATIMH010000028         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1159914, whole genome shotgun sequence.
ACCESSION   DATIMH010000028 DATIMH010000000
VERSION     DATIMH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159914"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="VLI90_00490"
     CDS             <1..1463
                     /locus_tag="VLI90_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSV12704.1"
                     /translation="TLGKIIKDAAGHPTPIHARTSWFTGLHPFLALRSIFFDKDYMPP
                     ELGALPPELQAWPFGWYLSSPASFYITFMFVLSLLLVMPSIILLRRMAQSTLTIKSWV
                     LTKLHINPGDQTHKPRTVWNNPIAWREAKTKASAARASLLRYGFIAAGILGALFLVIR
                     FAEVDEPAHYIDRNSYNDQAGVLTVYTKDTVTRYPVKANTRVTVNDITAPREGSPLDF
                     HGKYAIDLTTNARNEILTATLSPIPHKIKEEDVRNYLLGAVILEFAVILLIVTNAAAS
                     TVTREKEDGSLDLLLTTPITSRYYIWGKLSGLVGFILPLVAVPVISVLLFVVYDFFRV
                     VSSNGTDRWIAFPESIVIMPATLIIVAAFAAILGMQMSLRCRTTVLAVMSSVGIVAGV
                     CATLGWCGYTFLNFKNGGGISLFVGSFSPFTVLTMLINPYQFAERVFDTSDATSNRVW
                     IFISSFAAVGAYTAIVWAMYKSMVKNFDMTIRRQSR"
     gene            complement(1465..2502)
                     /locus_tag="VLI90_00495"
     CDS             complement(1465..2502)
                     /locus_tag="VLI90_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HSV12705.1"
                     /translation="MSKLQRCVVIGASAGGVEALRDVVARLPDDLPAPVFVVLHIPPY
                     VASSLPRILNRSGPLHAVHPKDGAIIKAGTIYVAPPDHHLLIEGQHVAVKKGPKENRF
                     RPSIDALFRSAAYTYGPRAIGVVLSGFLDDGTSGLWSIERLGGITVIQEPNEARFEDM
                     PRSALEFVNVNHTLPSTQIGALLGRLVTEPHPATKGPKHADDKERMQKEIQIAAEDSA
                     FQKGVMKLGELTPFTCPECHGVLVRIAEGKMSRFRCHTGHAYTDSALLEAVMESTGEM
                     LWQVIRSFEESLMLLNHMGNHLKKAGDPDRAETFFAKARELEKRSRTFHAAALEHESL
                     SGDNLGQQPET"
BASE COUNT          468 a          922 c          780 g          512 t
ORIGIN      
        1 acacgctggg caaaatcatc aaggacgccg ccgggcatcc gacgccgatt cacgccagga
       61 ccagctggtt tacggggctt cacccgttcc tggcgctgcg gtcgatcttc ttcgacaagg
      121 actacatgcc gcccgagctg ggcgcgctgc cgccggaact gcaggcttgg ccgttcggtt
      181 ggtacctcag cagccccgcg tcgttctaca tcacgttcat gttcgtcctc agcctgctgc
      241 tggtgatgcc cagcatcatc ctgcttcgcc ggatggcgca gtcgacgctg acgatcaaga
      301 gctgggtgct gaccaagctg cacatcaacc ccggcgacca gacacataag ccgcgcaccg
      361 tgtggaacaa tcccatcgcc tggcgtgaag ccaagaccaa ggccagcgcc gcccgcgctt
      421 cgctgctgcg ttacggcttc atcgccgccg gcatcctggg cgcgctgttc ctggtcatcc
      481 gcttcgccga agtcgatgaa cccgcccact acatcgaccg caattcctat aatgaccaag
      541 ccggcgtgct caccgtctac accaaggaca ccgtgacgcg ctatccggtg aaggcgaaca
      601 cgcgcgtcac cgtcaacgac atcaccgccc cgcgcgaagg cagcccgctg gacttccacg
      661 gcaaatacgc catcgacctg accaccaacg cccgcaacga aatcctcacc gccacgctca
      721 gcccgatccc gcacaagatc aaggaggaag acgttcgcaa ttacctgctc ggcgcggtca
      781 tcctggaatt cgccgtgatc ctgctgatcg tcaccaacgc cgccgcgtcc accgtcacgc
      841 gcgagaagga agacggcagc ctggacctgc tgctgacgac gccgatcacc agccgctatt
      901 acatctgggg caagctgagc ggcctcgtcg gcttcatcct gccgctggtc gccgtgccgg
      961 tcatcagcgt gctgctgttc gtcgtgtacg atttcttccg cgtcgtctcc agcaacggca
     1021 ccgaccgctg gatcgcgttc cccgaaagca ttgtcattat gcccgcgacg ctcatcatcg
     1081 tcgccgcctt cgccgccatc ttaggaatgc aaatgagcct gcgctgccga accacagtgc
     1141 tcgccgtcat gagcagcgtc ggcatcgtcg ccggcgtctg cgctacgcta ggctggtgcg
     1201 gctacacgtt cctgaacttc aaaaacggcg gcggcatctc cctcttcgtc ggcagcttca
     1261 gccccttcac cgtgctgacg atgctgatca acccctacca attcgccgag cgcgtcttcg
     1321 acacctccga cgcgacgagc aaccgggtgt ggatcttcat cagcagcttc gccgccgtcg
     1381 gggcatacac cgccatcgtg tgggcgatgt ataagtcgat ggtcaagaac ttcgatatga
     1441 cgattcgcag acagtcgaga tgaactaggt ttcgggttgt tgtccgaggt tatcgccgct
     1501 caggctttcg tgttcgagcg ccgcggcatg gaaggtgcgg gaccgcttct ccagctcgcg
     1561 cgcttttgcg aagaacgtct cggctcggtc cggatcgccg gcttttttca ggtggttccc
     1621 catgtggttg agcagcatca gcgattcttc aaagctgcgg atcacctgcc acagcatctc
     1681 accggtcgat tccatcaccg cttcgagcag cgcgctgtcg gtgtaggcgt ggcccgtgtg
     1741 acagcggaag cgcgacatct tcccctcggc gatcctcacc agcacgccgt ggcactcggg
     1801 gcaggtgaag ggggtcagct cgcccagctt catcacgccc ttctggaacg ccgagtcttc
     1861 cgcggcaatc tgaatctctt tttgcattcg ctctttgtcg tcggcatgtt tagggccctt
     1921 ggtcgccggg tgcggttcgg tgaccaggcg gcccagcagc gcgccgatct gggtcgacgg
     1981 caacgtgtga ttgacgttga caaactccag cgcgctgcgg ggcatgtcct cgaaccgcgc
     2041 ctcgttgggt tcctggatga cggtgatccc gccgaggcgt tcgatgctcc acaggcctga
     2101 cgtgccgtcg tcgagaaatc cgctgagcac caccccgatc gcgcgcggcc cgtacgtgta
     2161 cgccgccgag cgaaacagcg cgtcgatcga cgggcggaag cggttctcct tcggcccctt
     2221 cttcaccgcg acgtgctggc cttcgatcag caggtgatgg tccggtggcg cgacgtagat
     2281 cgtgcccgcc ttgatgatcg cgccgtcttt gggatgaacg gcatggagcg ggccgctgcg
     2341 gttgaggatc cgcggcagcg aactggccac gtaaggcggg atgtgcagga cgacgaacac
     2401 cggcgccggc aggtcgtccg gcagccgcgc gaccacgtcg cgcagcgcct ccaccccgcc
     2461 cgccgacgcc ccgatcacga cacagcgctg caatttgctc atgcggccct tctccaatgc
     2521 gcctgcggcg taacgcaacg aagttccccg gccgggcgga caccgcgacc acactttcga
     2581 atcacaacga ccgcgacacc cgcccggtcc gtggtagtgg cttgtcagcc gacggtgtca
     2641 tgtcgaaaac cgaatgactt cctctttggc ggcttctgcc tc
//
LOCUS       DATIMH010000029         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_871328, whole genome shotgun sequence.
ACCESSION   DATIMH010000029 DATIMH010000000
VERSION     DATIMH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871328"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VLI90_00500"
     CDS             <1..693
                     /locus_tag="VLI90_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HSV12706.1"
                     /translation="NPLMKVTDLTAIAALTRMAGPNIVTVCDGTFATPVLQQPLDCGI
                     DMVAHSTTKYIGGHSDVIGGALITRHDNYLFERARKSQHYGGSVPSPFDCWLTLRGID
                     TLPWRVRAQSENAMRIAQFLEQHAAVEKVHYPGLASHPGHKIAARQMSAFGGMLSFQT
                     NGTRETAMAVAARCKLFIRATSLGGAHSLIEHRASIEGPNTKTPQNLLRASIGLEHTD
                     DLIADLDQALAT"
     gene            complement(791..>3239)
                     /locus_tag="VLI90_00505"
     CDS             complement(791..>3239)
                     /locus_tag="VLI90_00505"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSV12707.1"
                     /translation="AGLLGRGGMGEVYRATDLALGQAVALKFLPETTASDERALARFY
                     NEVRVARQVTHPNVCRVYDVGVAEGLHYISMEFVDGEDLGQLLRRIGRLPADKAVETA
                     RKLCMGLGAAHEKGVLHRDLKPANVMIDGRGQIVIMDFGLAGIAEQLQGDIRSGTPAY
                     MSPEQLAGTEVTVKSDIYALGLLLYELFTGKRAFEAASLMELMQMQERAAPASITTIV
                     RDLDPAAERIIMRCLSHDPAQRPASAIAVAAALPGGDPLAAAIAAGETPSPELVAMAG
                     ETDGLMPKVAVSCLAAALVMILANMVISPRVSITSKINLENSPDALSRDARRHIQSFG
                     YTAKPGDAGWGLDYPSGYQSFVAKHPKEAAERWKNPGVGYPPLVQFWYRESPLPMPPQ
                     HQFNVALQYDDPPLERSGMVRLQTDTDGKLLEFEAVPPEVEKPAPAVPPFDWGKLFQA
                     AALDMTQFEQADAQWTPLANWDQRAAWTGTDASTGVSLRIEAAAWRGRPVFFKIIGPW
                     TAASRMAPLANSTGNSTPVLVIIYTVLIAACVLGWVNVRRGRADMRGATWLAGIFVVS
                     QAAGYLIGMHHTTSVNEFTAFWTAIGMAMVNGGLNWVVYVALEPWVRRKWPRTMIGWT
                     RFTSRGAGDPLVGRDLLYGSLLGAPLALGDAIVVALHGNNGMPAFPPLDALLGVKAEM
                     SAVLRSVAAAMFTALLFFFMLFLLRLLLRKDWIAGVAFVAVMTFATTPGSTTPLVDYP
                     LNALAYAIFAFMLLRFGLLAAMITSLIGQLLALGRMLDFSAWYAGMALMPFVLVVVVT
                     IYGFRKSLAGRKLFAEI"
BASE COUNT          669 a         1168 c          860 g          542 t
ORIGIN      
        1 aacccgctga tgaaggtcac cgatctgacc gccatcgcag cgctcacgcg tatggccggc
       61 ccgaatatcg tcaccgtctg cgacggcacc ttcgccacgc ccgtgctgca gcaacccctc
      121 gattgcggca tcgacatggt ggcccactcc accaccaaat acatcggcgg ccacagcgac
      181 gtgatcggtg gagcgctgat tacccgccac gacaactacc tgttcgaacg cgcccgcaag
      241 tcacaacatt acggcggcag cgtgccgtcg cccttcgatt gctggctgac cctgcgcggc
      301 atcgataccc tgccctggcg cgtacgcgcg caatccgaga acgccatgcg catagcgcag
      361 ttcctggagc agcatgcggc ggtagagaaa gtccactacc ccggcctcgc cagccacccc
      421 ggccacaaaa tcgccgcgcg ccagatgtcc gccttcggag gcatgctgtc gttccaaacc
      481 aatggaacgc gcgaaaccgc aatggcagta gccgcgcgtt gcaagctctt catccgagcc
      541 acgagcttag gcggagcgca cagcctgatc gagcaccgag ccagcattga aggcccgaac
      601 acgaaaacgc cccaaaatct cctccgagcc tccataggcc tcgaacacac cgacgacctc
      661 atcgccgatc tcgaccaggc cctagctacc taggttttct ctgcgtttcc tctgcgtctt
      721 tgcgtctctg cggtgagggg attttggggt aaagatccgt caccggagag acgcgataac
      781 aaccttttgc ctatatctcc gcgaacaatt tccggcccgc cagcgacttg cggaagccgt
      841 aaatcgtaac caccacgacc agcacaaacg gcatcaaggc catgcccgcg taccacgccg
      901 aaaaatccag catcctaccg agcgccagca actgaccaat caagctcgtg atcatcgccg
      961 ccagcaaccc aaaccgcaac agcatgaacg caaaaatggc atacgcgagc gcattcaaag
     1021 gataatcgac cagcggtgtc gtactccccg gggtggtcgc gaacgtcatc actgccacaa
     1081 acgctacgcc tgcaatccag tccttccgca gcaacagcct tagcaaaaac agcatgaaga
     1141 agaacaacag cgccgtgaac attgccgccg caaccgaacg caggactgcc gacatttccg
     1201 ccttcacacc cagcagcgca tccagcggcg gaaatgccgg cattccatta ttgccatgca
     1261 acgccaccac gatcgcatcc cccaaggcca gcggagcacc caacagggag ccatacaaaa
     1321 gatcgcgccc caccagcgga tcgcccgcgc cgcgcgacgt gaaccgcgtc cacccgatca
     1381 ttgtgcgcgg ccacttgcgg cgcacccacg gctccagcgc aacataaacc acccaattca
     1441 aaccgccatt taccatggcc ataccgatcg ctgtccagaa ggccgtaaac tcgttgacgc
     1501 tcgtcgtgtg gtgcatgccg atcagataac ctgccgcctg cgacacgacg aagattcccg
     1561 ccagccacgt cgcgccgcgc atgtccgccc ggccgcgcct cacattcacc cagcccagga
     1621 cacaagccgc aatcagcacg gtgtaaatga ttaccagcac cggcgtcgaa ttcccggtac
     1681 tgttcgcgag tggcgccata cggctggccg ccgtccatgg cccaataatc ttgaagaata
     1741 ccggccgccc ccgccacgcc gccgcttcaa tccgcaggct caccccggtg gacgcgtccg
     1801 tcccggtcca tgcagcgcgc tgatcccaat tcgccagcgg cgtccactgc gcgtccgctt
     1861 gctcgaactg cgtcatatcg agcgcggccg cctgaaacag cttgccccaa tcgaatggcg
     1921 gaacagcggg cgcaggcttc tcgacttccg gcgggaccgc ttcgaattcc aataatttcc
     1981 cgtcggtgtc ggtctgcaga cgcaccatgc ccgagcgctc tagcggcggg tcgtcatact
     2041 gcagcgccac gttgaactga tgctgcggcg gcatcggcag cggactctcc cgataccaga
     2101 actgcaccaa tggcgggtac ccaacgccgg gatttttcca gcgctcggcg gcttcctttg
     2161 gatgcttggc cacgaagctc tgataacccg acgggtagtc caggccccat cccgcgtccc
     2221 ccggcttggc ggtgtaccca aagctctgta tgtgcctgcg agcatcccgc gatagcgcgt
     2281 ccggcgaatt ctccagattg attttcgaag tgatcgacac gcgcggcgag atcaccatat
     2341 tcgccaggat catcaccagc gccgcggcca aacaggaaac cgccaccttg ggcatcagcc
     2401 cgtcggtctc gcccgccatc gccaccagtt cgggcgacgg agtctcgccc gccgcgattg
     2461 ccgccgccag tggatcgccg cccggcaacg ccgccgcgac cgcaatcgcc gaggcgggcc
     2521 gctgcgccgg atcgtgactc aggcagcgca tgatgatacg ttccgccgcc ggatcgagat
     2581 cgcgcacgat cgtcgtgatg ctcgccggcg cggcgcgctc ctgcatctgc atcagttcca
     2641 tcagcgacgc cgcttcgaac gcccgtttgc cggtgaacaa ctcatagagc agcagaccga
     2701 gcgcgtagat gtcgctcttt accgtgactt cggtgccggc caattgctct ggcgacatat
     2761 acgccggcgt gccgctgcgt atatcgccct gcagttgctc ggcgatcccg gcaagtccga
     2821 aatccatgat cacgatctgg ccgcgcccgt cgatcatcac gttggccggt ttcaaatcgc
     2881 gatgcaggac gcccttctca tgcgccgcgc cgagacccat gcacagcttg cgcgccgtct
     2941 ctaccgcttt gtccgccggt agcctgccta tccggcgcag caattgcccc agatcttcgc
     3001 catcgacgaa ctccatcgag atgtagtgca gtccctcggc gaccccgacg tcgtacacgc
     3061 ggcagacatt cggatgggtc acctgccgcg ccacgcgcac ttcgttatag aatcgcgcga
     3121 gtgcgcgttc atccgacgca gtcgtttcag gcaggaactt cagcgccact gcctggccca
     3181 atgcgagatc ggtagcgcga taaacttcgc ccatgccgcc gcgcccgagc aatcccgcg
//
LOCUS       DATIMH010000030         5425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4047887, whole genome shotgun sequence.
ACCESSION   DATIMH010000030 DATIMH010000000
VERSION     DATIMH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4047887"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1254
                     /locus_tag="VLI90_00510"
     CDS             151..1254
                     /locus_tag="VLI90_00510"
                     /inference="COORDINATES: protein motif:HMM:NF003711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HSV12708.1"
                     /translation="MILKIEKDHQRFRQIVKGRIRDDLRKFLTKGELIGREGKHLISI
                     PVRGVELPHFRYGDNSQGVGAGDGKEGDVVGKGQPGQGKSQGGTDPGAHIMEVDLSLD
                     ELADILAEELKLPRIEPKGKHRITTVREKYSSLRRVGPESLRHFKRSFKEALKRQIML
                     GNYDPDHPVIIPEKKDIRYRSWKEIKAPQSNAVIIFMMDVSGSMGEEQKELVRLESFW
                     IDTWLRKNYEGIESRYIVHDVSAKEVDKHTFYHLREDGGTKISSAYRAAKNLLDSHYS
                     PDEWNIYLFHFSDGDNSSEADSRDCVKLLKEQLLPICNMFGYCQVASAYGSGNFINVL
                     HEHLRNHERVLTSRVNSKDDIYDSIKTFFGKGH"
     gene            1306..1650
                     /locus_tag="VLI90_00515"
     CDS             1306..1650
                     /locus_tag="VLI90_00515"
                     /inference="COORDINATES: protein motif:HMM:NF042561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6678 family protein"
                     /protein_id="HSV12709.1"
                     /translation="MTKDLAGLGSGNMNTTKWAEFFKILFDHGQADVTVQIKLLYDEK
                     AFSFKKFWCPTRRYTEVGGMPFQNREIEWLEIRGAGAADILNKLMRLGQLPIVKRSEG
                     FRLQAYGSISLT"
     gene            1683..2237
                     /locus_tag="VLI90_00520"
     CDS             1683..2237
                     /locus_tag="VLI90_00520"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV12710.1"
                     /translation="MTLTPNKLLTADELLAMGDIGRCELLYGQLVMMSPAGAEHGVVA
                     LRFGRYLSDFVEQHDLGVVFAAETGFKIEANPDLVRAPDASFVRKSRLTSPLPRGYFP
                     GVPDLAVEVNSPDDSKRAVAEKINMWLAHGTTLVWEVDPPTMIVHVHRVGEQPRRLTQ
                     SDTLTAEPLLPRFILSLQRVFRNP"
     gene            2277..3059
                     /locus_tag="VLI90_00525"
     CDS             2277..3059
                     /locus_tag="VLI90_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12711.1"
                     /translation="MIATAPPVDTSPSNPNDCACLLKGRAAMPDNNPTGIRRFKGPDG
                     RERCELEYEIIEGPLPDATPDLPLEVERQYDQLHDLTINEPTKAVPRLEPLVAKYPQL
                     AVLMNWLASSYAATGRQAEADALNERLWREHPDYLFARIARADAHLSKGELDQVPVAL
                     GGFDLKLIYPHRNVFHISEAVALWSVICQWLFRRGEFDRAEFYLEQIANIAPDDPAVE
                     TLKDMMVPYVLEKAVKSLMERAQRPRRPRKKSATKKRKPRGG"
     gene            3077..5305
                     /locus_tag="VLI90_00530"
     CDS             3077..5305
                     /locus_tag="VLI90_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HSV12712.1"
                     /translation="MKLIFLVVSLSIAASTFAADPSPIYLDPSCPLEARVDDLLARLM
                     LEEKISLIHGDSKFTTAAIPRLGIPRRWLSDGPHGVREDVGPDTWNPAGRTDDYCTAM
                     PCDLLLASTWDPQDARAFGTVIGQEARARGKQIMLGPSVCIQRTPLCGRNFEYMGEDP
                     FLTSRIAVGYIQGEQAQGVSSCVKHFACNNQEIERNSIDVEIDERPLREIYLPAFEAA
                     VKEAGVLSVMGAYNQLRGQHCCHNDYLLNQILKGEWNFQGLVVSDWGGVHDTKQAVLN
                     GLDLEMGTNKPYDEFYLAQPFIDGLRSGEFPTAVLDDKVRRNLRQMFAGHVFDRDGPP
                     GSLNTKEHQDAARKIAEDGIVLLKNDDGALPLDPAKIKSIAVIGDNAVHKFCHEGGSA
                     ELKSLYEITPLDGILHRVGNKVDVTFSEGFEPPPQTRRRRRPTTAASGVEAPQPTTAQ
                     LDDAAAAMIERAVRAAKQSDVAIVVAGLNHNWQFDTEGSDRKDMRLPFKQDELIRRVV
                     EANPRTVVVLISGGAVGMSEWIDRVPAIVQAWYPGLEGGNALASVLFGDVNPSGKLPC
                     TFPRQLQDSPAHALGAYPGKDGVERYEEGLLVGYRWFDTKQIEPLFPFGHGLSYTKFE
                     YANLQLHETGTELTVEADITNAGERAGAEIVQVYVQPLHPSVERPAKELKGFAKVSLK
                     PGEKQAATITLPPRAFAYYDPTKKSWVAEAGEYAILVGASSRDVRLRAEYKIPQTIAL
                     GN"
BASE COUNT         1134 a         1670 c         1661 g          960 t
ORIGIN      
        1 gccgcgcgct aggacatccg cgtcgtcgga gtacctcttc ggagtacctc ttcggagtac
       61 ctccgaaccc ggcgtggtcg atttcctgct ccgtacgagt ccgatttatc cttcgtccca
      121 acccttcacc gtggatacaa tccagatggc atgatcctca agatcgaaaa agatcaccag
      181 cggttccggc agatcgtcaa aggccgcatt cgtgacgacc tgcggaagtt tttgaccaag
      241 ggtgaattga tcggccgcga gggtaagcac ctgatcagca tccccgtccg cggcgtcgag
      301 ctgccgcact tccgttacgg cgacaacagc cagggtgtcg gcgccggtga tggcaaagaa
      361 ggcgacgtgg tcggcaaagg ccagcccggc cagggcaaaa gccagggcgg caccgatccg
      421 ggcgcgcaca tcatggaagt cgatctgtcg ctggacgagc tggccgacat cctggccgaa
      481 gagctgaagc tgccgcggat cgagcccaag ggaaagcacc gcatcacgac ggtgcgcgag
      541 aaatattcat cgctccgccg ggtgggcccc gagtcgctcc gccacttcaa gcggtcgttc
      601 aaggaagcgc tcaagcggca gatcatgctc ggcaattacg accccgatca cccggtgatc
      661 atccccgaga aaaaggacat ccgctatcgc agctggaagg agatcaaagc cccgcaatcc
      721 aacgcggtca tcatcttcat gatggacgtt tccggctcga tgggcgaaga gcaaaaggag
      781 ctggtccgcc tggaaagctt ctggatcgac acctggctgc ggaagaatta cgaaggcatc
      841 gaaagccgct acattgtgca cgatgtcagc gcgaaggaag tggacaagca cacgttctat
      901 cacctgcgcg aagacggcgg caccaagatc agcagcgcct atcgcgcggc caagaacctg
      961 ctcgactccc actattcccc cgacgagtgg aacatttacc tgttccactt cagcgacggc
     1021 gacaacagta gcgaagcaga cagccgcgac tgcgtgaaac tgctgaagga gcagctgctg
     1081 ccgatctgca acatgttcgg ctactgccag gtcgccagcg cctacggcag cggcaacttc
     1141 atcaacgtgc tgcacgagca tctgcgcaac cacgaaaggg tgctgacgag ccgcgtgaat
     1201 tcgaaggacg atatttacga ttcgatcaag acgttcttcg ggaaggggca ttaggtgacg
     1261 actgcagagc ggttggaaat gttgggtcgc gcacatgata agcgcatgac caaagatctt
     1321 gccggactcg gaagcggcaa catgaacacg accaaatggg ccgagttttt caagatcctc
     1381 ttcgaccatg gccaagcgga cgtgacagtg caaattaagc tcttgtacga tgagaaggct
     1441 ttttcgttca agaagttctg gtgtccgact cgtcgttaca ccgaggtcgg gggaatgccg
     1501 tttcagaacc gcgagatcga atggcttgag atccgcggtg cgggagccgc cgatattctg
     1561 aacaagctga tgcggctggg acaattgccg attgtgaagc ggagtgaagg ctttcggtta
     1621 caagcttacg gatcgatttc gctgacctaa ggcgggcttt agcccgacgc actcggcaag
     1681 ccatgacgct gacacccaac aaactcctca ccgccgacga actgctcgcc atgggcgata
     1741 tcggccggtg tgaactgctc tacgggcaac tggtgatgat gagtcccgcg ggtgccgagc
     1801 atggcgtcgt cgcgttgcgc ttcggccgtt atctgtcgga cttcgtcgag cagcatgacc
     1861 tcggcgtcgt cttcgccgcg gagacggggt tcaagattga agccaacccg gacctcgttc
     1921 gcgcgcccga cgccagcttc gtccgcaagt ctcgcctcac gagtccgctg ccgcggggtt
     1981 atttccccgg cgtgccggac ctggcggtgg aagtgaattc gccagacgat tccaaacgag
     2041 ccgtcgcgga gaagatcaac atgtggctgg cgcacgggac gacgctggta tgggaagtcg
     2101 acccgccgac aatgatcgtt catgttcatc gtgtcggtga acagccgcgg cgtttgaccc
     2161 agtcggacac gctcacggcc gaaccgctgc tgccgcggtt cattctttca ctgcagcgcg
     2221 tcttcaggaa tccgtgacag agtgggcaga cgtgccgccg cgactttgac gcgctgttga
     2281 ttgcaactgc tccgccagtt gacacgtccc cttcaaatcc gaacgattgc gcatgcctcc
     2341 tgaaaggacg tgcagccatg cccgacaaca accccaccgg cattcgccgc ttcaagggtc
     2401 ctgatggtcg cgaacgttgc gagcttgaat acgagatcat cgaaggcccg ctgcccgatg
     2461 cgacgcccga cctgccgctg gaagtggagc ggcaatatga ccaactgcac gatctgacga
     2521 tcaatgaacc caccaaggcc gtgccccggt tggaaccgct ggtggcaaaa tatccgcaac
     2581 tggcggtgct gatgaactgg ctggcctcct cgtatgccgc caccgggcgc caggcagagg
     2641 ccgatgcgtt gaacgagcgg ctatggcgcg agcacccaga ctacctcttc gcccgcatcg
     2701 cccgcgccga tgcgcatctc agcaaggggg agttggatca ggtgcccgtt gcgctgggcg
     2761 gctttgattt gaagttgatt tatccgcacc ggaacgtgtt ccacatcagc gaagcggttg
     2821 cgctctggtc ggtaatctgt cagtggctct tccgccgcgg cgagttcgac agggctgagt
     2881 tttatttgga gcaaatcgcc aacatcgcgc ccgacgaccc ggccgtcgaa accttgaaag
     2941 acatgatggt gccgtacgtt ttggaaaagg ccgtaaagtc gctgatggaa cgggcccagc
     3001 gcccgcggcg gccgcggaaa aagtctgcaa caaagaagcg aaagccacgg ggcggctgag
     3061 ttgacgggaa gcgcgcatga agctcatatt tcttgttgtg tcgctctcta ttgccgcgtc
     3121 cacgtttgcc gctgatccgt cgccgattta tctcgatcca tcctgcccgc tcgaagcccg
     3181 cgtcgacgac ctcctcgccc gcctcatgct cgaagaaaaa atctccctca tccacggcga
     3241 ttccaaattc accaccgcgg cgattcctcg cttaggcatt ccgcggcggt ggctttccga
     3301 tgggccgcac ggcgtgcgcg aagatgtcgg gccggacacg tggaaccccg cggggcgtac
     3361 cgatgattac tgcaccgcca tgccgtgcga cctgctgctc gccagcacct gggacccgca
     3421 agatgcgcgc gcgttcggca ccgtgattgg ccaggaagcg cgggcgcgcg gcaagcagat
     3481 tatgctcggc cccagcgtct gcattcagcg cacgccgctg tgcgggcgga atttcgagta
     3541 catgggcgaa gacccgttcc tcacgtcgcg catcgctgtc ggctacatcc agggcgagca
     3601 ggcgcagggc gtctcgtcct gcgtcaagca tttcgcctgc aacaatcagg agatcgaacg
     3661 caattcgatc gacgtcgaga tcgacgagcg cccgctgcgc gagatctatc tgcccgcgtt
     3721 cgaggcggcc gtgaaggaag ccggcgtctt gtcggtcatg ggcgcgtaca accagctgcg
     3781 cggccagcac tgctgccaca acgattacct gctcaaccag atccttaaag gcgaatggaa
     3841 cttccaaggc ctggtcgtct ccgattgggg cggcgtgcac gacacgaagc aggcggtgct
     3901 caacggtctc gatctggaga tgggcacgaa caagccgtac gacgaatttt atctcgcgca
     3961 gccctttatc gacggcctgc gcagcggcga atttccaacg gcggtgcttg acgacaaggt
     4021 ccgccggaac ctgcgccaga tgttcgcggg gcatgttttc gaccgggatg gtccaccagg
     4081 ctcattgaac acaaaagagc accaggacgc ggcaaggaag atcgccgaag acgggatcgt
     4141 cctgctgaag aacgacgatg gcgcgctgcc gctggacccg gcgaagatca aatcgatcgc
     4201 cgtcatcggc gacaacgcgg tgcacaagtt ctgccacgaa ggcggatccg ccgagctgaa
     4261 atcgctctac gaaatcaccc cgctggacgg catcctgcat cgcgtgggca acaaggtgga
     4321 cgtgacgttc tcagagggct tcgaacctcc accgcaaacg cgccggcgcc gccggccgac
     4381 gaccgcggcc agcggtgtcg aggccccgca gccgaccacc gctcaactcg atgacgctgc
     4441 cgccgcgatg atcgagcgcg cggtgcgcgc cgccaaacaa tccgacgtcg ccatcgtcgt
     4501 cgccgggctg aatcacaact ggcagttcga caccgaaggc tccgaccgca aggacatgcg
     4561 gctgccgttc aagcaggatg agctgatccg ccgggttgtc gaagccaacc cgcgcacggt
     4621 cgtcgtcctg atctccggcg gggcggtggg aatgtccgaa tggatcgacc gcgtccccgc
     4681 gatcgtgcaa gcatggtacc ccggcctcga aggcggcaac gcgctggcgt cggtcctctt
     4741 cggcgacgtc aaccccagcg gcaaactccc gtgcacgttc cccaggcagc tgcaagattc
     4801 ccccgcccac gcgctcggcg cctaccccgg caaagacggc gtcgaacgct acgaagaagg
     4861 cctgctcgtc ggctaccgct ggttcgacac caagcagatc gagccgctgt tccccttcgg
     4921 ccacggtttg tcgtacacga agttcgaata cgcgaacctg cagcttcacg aaaccggaac
     4981 cgagctgacg gtggaagctg acatcacaaa cgcaggcgaa cgagcgggcg cggagatcgt
     5041 gcaggtctac gtgcagccgc tgcatccgag cgtcgagcgt ccggcgaagg aacttaaagg
     5101 ttttgcgaaa gtctcattga aaccgggcga gaaacaggcc gcaacgatta cgctgccgcc
     5161 gcgggcgttt gcgtattacg acccgacgaa gaagtcgtgg gtggccgagg cgggggagta
     5221 cgcgatcttg gtgggcgcgt cgtcgcggga tgttcgtttg cgcgcggagt ataagattcc
     5281 gcagacgatc gcgctcggca attgaagccg cgttggatgc gggcgccgcc ataatagccg
     5341 caggcgacag ccagcggggg cgcggaaact cgagcggcgc cgattaacaa acctcccgct
     5401 ggctgtcgcc tgcggctatt aaatg
//
LOCUS       DATIMH010000031         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1738358, whole genome shotgun sequence.
ACCESSION   DATIMH010000031 DATIMH010000000
VERSION     DATIMH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738358"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1883
                     /locus_tag="VLI90_00535"
     CDS             120..1883
                     /locus_tag="VLI90_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV12713.1"
                     /translation="MSSAVRSTTLAPTANARRANRTIVLALLALVFAVFGRSIFFDFS
                     GFDDFLHVVDNPYFHPINAQHLGQLWTHLYHAYMPVTFTVWAALAKTGELPTGDVQGV
                     RFNPYLFHAANVVLHAINVLLVWRILRRFVKSPWPAAAGAAVFAVHPLQVESVAWISS
                     MKDVLGGMLALASVLVYLHFVDRPRAWWQYALATLLFTLAVLSKGTTIVTPALVVILL
                     LLERGPREWRAWFLPLAPWFAAAPPYLWITSRQQGDAPLLVWSPLWFRPIEAADALAF
                     YFRKLIWPVDLAIDYGRTPRWVMDHGTAALACLVVVVVFATATWQWRRRPALLAGLAL
                     FVVALAPVLGLVPFIFQVYSTVADRYLYLPMVGVALVVADAMERGGRTARVVVITMIA
                     ILSVLCVRQVGIWRDSASLFTHAIAVNPSNVIGWHGLGTVAEENRDLPAAVNDYRQAV
                     QNRPDELQSLSSLAHALVRLKGPNDPETKRAYAAFLREGARRVLLRYPNSPLAHRKLA
                     EALQMEGRTDEATAEFQRAIAVDPKDARSRVEFGQFLEATGHAPQAIEQYQIALQLHP
                     ADAATVQRLARLRLAEPATLP"
BASE COUNT          330 a          648 c          729 g          405 t
ORIGIN      
        1 gggcacgtgt ttttgcgccc gaacgaaaac acgtgcccct tcggggctac cgctacgcgg
       61 cggagggatg cggcgcgcgc gttacgcgac ggaagcgcgc ttgcattaca ctgcacgcgg
      121 tgtcatccgc ggtccgttca acaacactcg ccccgactgc gaatgctcgt cgcgccaacc
      181 gaaccatcgt cctcgccctg ctcgcgctcg tcttcgccgt cttcggccgg tcgatcttct
      241 tcgacttcag cggcttcgat gatttcctgc acgtcgtcga caacccatat tttcatccga
      301 tcaacgcgca gcacctgggg cagctgtgga cacacctgta ccacgcgtat atgccggtga
      361 cgtttacggt gtgggccgcc ctggccaaga cgggcgagct gccgacgggg gatgtgcagg
      421 gcgtgcgatt caatccgtac ctgttccacg cggcgaacgt ggtgctgcac gcgatcaacg
      481 tgctgctggt ctggcggatc ctgcggcggt tcgtgaagtc gccgtggccc gccgccgcgg
      541 gggcggcggt gttcgcggtg catccgctgc aggtggagtc cgtcgcgtgg atctcgtcga
      601 tgaaggacgt gctcggcggg atgctggcgc tggcgtcggt attggtgtac ctgcactttg
      661 tcgatcggcc gcgggcgtgg tggcaatatg cgctggcgac gctgctgttc acgctggcgg
      721 tgctgtcgaa ggggacgacg atcgtcacgc cggcgctggt ggtgatcctg ctcctgctcg
      781 agcgcggccc acgagagtgg cgcgcttggt ttctgccgct ggcgccatgg ttcgccgctg
      841 ccccgccgta tctctggatc accagccggc aacagggcga tgcgccgctg ctggtctgga
      901 gcccgctgtg gtttcgaccg atcgaggcgg ccgatgcgct ggcgttttat tttcggaagc
      961 tgatttggcc ggtggatctc gccatcgact acggtcgcac accgcgatgg gtgatggatc
     1021 acggcacggc ggcgctggcg tgccttgtgg tggtcgtcgt ttttgcgacg gcgacatggc
     1081 aatggcggcg aaggccggcg ctgttggcgg ggttggcgct gttcgtcgtc gcactggcgc
     1141 cggtgcttgg actggtgccg ttcatctttc aggtctactc gacggtggcg gatcgatacc
     1201 tgtacctgcc gatggtgggt gtcgcgctgg tcgttgccga tgctatggag cgcggcgggc
     1261 gaacggcccg cgtggttgtc atcacgatga tcgcaattct gtcggtgctg tgcgtgcgac
     1321 aggtgggcat ctggcgcgat tcggcgtcgc tgttcacgca cgccatcgcg gtcaatccgt
     1381 cgaacgtcat cggctggcac ggtctgggca cggtggcgga ggagaaccgc gacctgccgg
     1441 cggcggtgaa cgactaccgc caagccgtac aaaatcggcc cgatgaactg cagtcgctga
     1501 gcagcctggc gcacgcgctg gtgcggttga agggtccgaa tgatcctgaa acgaagcggg
     1561 catacgcggc gtttctgcgc gaaggggcgc gacgcgtatt gctgcgatac ccgaactcgc
     1621 cgctggccca tcgcaagttg gcggaggcat tgcagatgga aggccgaaca gacgaagcga
     1681 ccgccgaatt ccagcgggcg atcgcggtgg atccgaagga tgcgcgatct cgcgtggagt
     1741 tcggccaatt tctggaggcc acgggccacg caccgcaggc gatcgagcaa tatcagatcg
     1801 ccctgcagct tcatcccgcc gatgccgcga ccgtccagcg gctggcgcgc ctgcggctag
     1861 ccgagcccgc gacgctgccg tagaatgctg cgctccagcg attgtcagct ggttccatca
     1921 accgagcagg aagaaccgca atggccatca aagtccgcat cccgtcgccg ctccgcaact
     1981 acaccaacgg cgctgatatc gtcgaagccg agggcggcag cgttggcgaa gtgctaaacg
     2041 cgctgaagag ccacgccgcc ggcatcgagc agcggctgtt caaaggaccg aaccagctga
     2101 accggttcgt ga
//
LOCUS       DATIMH010000032         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3182892, whole genome shotgun sequence.
ACCESSION   DATIMH010000032 DATIMH010000000
VERSION     DATIMH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3182892"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..698)
                     /locus_tag="VLI90_00540"
     CDS             complement(78..698)
                     /locus_tag="VLI90_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV12714.1"
                     /translation="MSHSSMSGPCRQVLIVEDESRLREMLAHAITDMGFDVSTARSAE
                     AAVKMLDQQPADIILLDLNLPGINGIELFEIIHKRWPAIQVIVLTGFGDLDVAKRAIR
                     LDVSDFLTKPCPLGDLEIALDRARHRRLVAIDPTYLAPEAPPTPTPEPSKSAAPMTLE
                     EIERAHILAALERNGGNRTATAQELGISLRTLYYRLETYQKQGLNV"
     gene            complement(695..2269)
                     /locus_tag="VLI90_00545"
     CDS             complement(695..2269)
                     /locus_tag="VLI90_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSV12715.1"
                     /translation="MTLGRKILIKNAALLVALVVLACVATQGLLRLRASTRTVLTAYR
                     QLQRIETAETDVTAAKSGLVRGQTAGPALTAPLQSAVTRIDEFFAVTGYDYSTGDAAM
                     AIADVRQASNALTDLVALANEPVASVAQAARLSDMSTRTDAALALLHQAATSCDKFIR
                     AQEAGTEQQFHTTLILVVILSSVILLTAILLSASQYRSSVLPLRRIREGVRKMSRGQF
                     QERLALANVEPEFAGLVDEFNAMAAKLDEFYHELERKVAQKSRELVRSERLASVGFLA
                     AGVAHEINNPLNIISGYAELSLKKMSADLAGPATRGRVTPATIAGRTGASDPIKSLQI
                     IRDEAFRCKEITQKLLSLARGGSDARVPVSLPKAAEEVATMVGGLSQYRDRRLVLGFD
                     DSSALVVMANVTEIKQVLLNLTVNALEATPPGTGEVRIQGRWRDGAIELSVRDNGRGM
                     SRQTIERVFEPFYTERRGTIDDRSGTGLGLTISHAIVESHGGQLRAESEGPGMGSEFV
                     IRLPAASREAELETVL"
     gene            2515..3663
                     /locus_tag="VLI90_00550"
     CDS             2515..3663
                     /locus_tag="VLI90_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HSV12716.1"
                     /translation="MAYLGRSKASLVAAAAVVGLAGSAALAGTDFYKQVNLVSDGSIA
                     GTTGDPDLVNPWGVAFAPGGEFWISDNVSNNISLYDGAGNKFAPSIPVSGNAMGGAAP
                     TGQVYNGTGEFGGSLFILSGEGGAINGWNGSGNATTLIDNSTSGTVYKGLAIGNSGGK
                     NLIYASDFNHGVVSVYGGDLKAATLAAGAFTDANLPAGYAPFNVQNINGNIYVTYALQ
                     DAAKHDDIAGAGHGFVDVFGADGTFMQRLISGGALDSPWGIAMAPANFGKFSNDLLVG
                     NFGDGKINAFNPTSGALLGTLSDNNGKPIVLGDLWAITFGGGGLDGATNQLFFTAGVS
                     EESHGLFGHLDAAASGGQAVPLPNMLYVLPVALALAGVSAKRLRAREA"
     gene            complement(3851..>4517)
                     /locus_tag="VLI90_00555"
     CDS             complement(3851..>4517)
                     /locus_tag="VLI90_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12717.1"
                     /translation="VPGTSVLRCPEGQDYQVTLMNGSPYSNGAKAPTDDLANDGYFAD
                     GHGVTPAIIPRVDGQPAYAVCSWYALNMRVAFSAFTQWPGKTDSAGLGATPFVSYVGW
                     ANKTTAPLSQGLSNPLLARKITEIKHSANMVMLAEADSMNWTDQSSSPANASLMVVQM
                     AARHGQKTTDKLNAYTNLAFMDGHVLLLPTAPLTVKSSTAKLYPLAEVPLSWIFFFLN
                     YDK"
BASE COUNT          750 a         1413 c         1509 g          845 t
ORIGIN      
        1 cccggattct gccgcgcggg caagcatccg ggtcgttgga gtacatccga acccggcgtg
       61 ggtgggctcc aaatcgttca gacgttcaac ccctgcttct gatacgtctc cagccgatag
      121 tagagcgtcc gcagactgat ccccagttcc tgcgccgtcg cggtgcgatt gccgccgttg
      181 cgctctaatg cggccaggat atgggcgcgc tcgatctctt ccagcgtcat cggcgcagcc
      241 gacttgctcg gttccggcgt cggcgtgggc ggggcttcgg gggcgagata agtgggatcg
      301 atcgcgacga gccggcggtg tctggcgcgg tccagcgcga tctccagatc gcccagcggg
      361 cagggcttgg taaggaagtc gctaacgtct aggcgaatcg cgcgcttggc gacgtccaga
      421 tcgccgaaac cggtcaggac gatcacctgg atcgccggcc agcgcttgtg gatgatctcg
      481 aacagctcga tgccgttgat gccggggagg ttcaggtcca gcaggatgat gtcggccggc
      541 tgctggtcga gcatcttcac cgcggcctcg gccgagcgcg cggtgctcac atcgaaaccc
      601 atatcggtga tcgcgtgcgc gagcatctcg cgcagtcggg attcatcttc gacgatcagc
      661 acctgccggc acgggccgga catggacgaa tggctcataa caccgtctcc agctccgcct
      721 cgcggcttgc ggccggcagg cgaatcacaa actcgcttcc catccccggt ccttcgcttt
      781 cagcgcgcaa ttgcccgccg tggctttcga cgatcgcgtg gctgatcgtc aggcccaatc
      841 ccgttccgct gcggtcgtcg atggttccgc gccgctcggt atagaacggt tcgaagacgc
      901 gctcgatcgt ctggcggctc atgccgcggc cgttgtcgcg cacggatagt tcgatcgcgc
      961 catcgcgcca ccggccttgg atgcgcactt ccccagtgcc cggcggcgtc gcttccagcg
     1021 cgttgaccgt caggttcagc agcacttgtt tgatctcggt gacgttggcc atgacgacca
     1081 gtgccgagct gtcgtcgaag ccaagcacca gccggcggtc gcgatattgg ctgagcccac
     1141 ccaccatcgt cgcgacttct tccgccgcct tgggcagcga gacgggaacg cgagcatcgc
     1201 tgccgccgcg ggccagcgac agcagttttt gcgtaatctc tttgcagcgg aacgcctcat
     1261 cacgaatgat ctgcagcgac ttgatcgggt ccgaggctcc ggttcggccg gcgatggtgg
     1321 ccggggtgac tcgcccgcgc gtcgccgggc cggcgagatc ggcactcatc ttcttcagcg
     1381 acagctcggc atagccggag atgatgttga gcgggttgtt gatctcatgc gcaacgcccg
     1441 cggcgaggaa gccgacgctg gcgaggcgct ccgatcgcac cagctcgcgc gacttctggg
     1501 cgaccttgcg ttccaactcg tgatagaact cgtcgagctt cgcggccatc gcgttgaact
     1561 catccaccag cccggcgaat tccggctcga cgttcgccag tgccaagcgt tcctgaaatt
     1621 gcccgcgcga cattttgcgg acgccttcgc gaatccgccg aagcggcagc acgctgctgc
     1681 ggtattgcga agcgctgagc aagatcgccg tgagaaggat gacgctcgac aggatgacga
     1741 cgaggatcag cgtcgtgtgg aactgctgtt cggtgcccgc ctcctgagcg cggatgaact
     1801 tgtcgcagga ggtggctgcc tggtgtagca gcgccagcgc cgcatcggtg cgggtggaca
     1861 tgtcgctcag ccgcgccgct tgcgcgacgg acgccacggg ttcattcgcc agcgcgacca
     1921 ggtcggtcag cgcgttgctc gcctggcgca cgtcggcgat ggccatggcg gcgtcgccgg
     1981 tgctgtagtc gtagccggtg acggcgaaga actcgtcgat gcgcgtcact gccgactgaa
     2041 gcggggcggt gagcgccgga ccagcggtct gcccgcggac caacccgctc ttggcggcgg
     2101 tcacgtcggt ctcggcggtc tcgatgcgct gcagttggcg ataggcggtg aggaccgtgc
     2161 gcgtgctggc gcgcaggcgc agcagtccct gcgtggccac gcacgccaac acgaccagcg
     2221 cgaccaacaa ggcggcgttc ttgatcagga ttttccgacc gagtgtcatg gtgccaccag
     2281 ccaggtgagg gactcacggg ggcggccgca gcaaaggccg ttgtcccccg ggttcgcgcg
     2341 cgtgcgccca catcttaccc gacacactcc ccagtggatg ttgggcccaa ctgtaaacgc
     2401 agggcgtcgc cgggggcgtc atctttgcaa actttgcaat tatggcgatc tcttgtgctt
     2461 ggcgtgctta ttctgggact tggcgcacgc gaattgaaac aaggagaagg caagatggct
     2521 tatctgggaa gaagcaaggc atcactggtt gccgccgcgg cggtggtggg gctggcgggt
     2581 agcgccgcct tggcgggcac ggacttttac aagcaggtga acctcgtctc cgatggatca
     2641 atcgccggca cgacgggcga tccggatctc gtgaatcctt ggggcgtcgc attcgcgccc
     2701 ggcggcgagt tctggatttc cgacaatgtg agcaacaaca tctcgctgta tgacggggcc
     2761 ggcaataaat tcgccccatc gattcccgtc tctggcaacg ccatgggcgg cgcggcaccc
     2821 acgggccaag tctacaacgg caccggtgag ttcggcggga gcctgttcat cttgtccggt
     2881 gaaggcggcg cgatcaacgg ttggaacggc agcgggaacg cgacgacgct gatcgataac
     2941 tcgaccagcg gcaccgttta caagggcctg gcgatcggca acagcggcgg gaagaatctg
     3001 atctacgcca gcgacttcaa tcacggcgtc gtgtcggtgt acggcggcga cctgaaagcc
     3061 gcgacgctcg ccgcgggcgc gttcaccgat gccaatctgc ccgcgggtta cgcgccgttc
     3121 aacgtgcaga acatcaacgg caacatctat gtgacctacg cgctgcagga tgcggccaag
     3181 cacgatgaca tcgccggcgc cggtcacggc ttcgtcgatg tcttcggcgc cgacggcacg
     3241 ttcatgcagc ggttgatctc cggcggcgcg cttgattcgc cgtggggcat cgcgatggcc
     3301 ccggcgaact ttgggaagtt cagcaacgac ctgctcgtcg gtaattttgg cgacggcaag
     3361 atcaacgcgt tcaatccgac cagtggcgcg ctgctcggca cgctgtcgga caacaatggc
     3421 aagccgatcg tgctgggcga tctgtgggcg atcacgtttg gtggcggagg gctcgacggg
     3481 gcgacgaatc agctgttctt caccgcaggg gtgtctgagg agtcacacgg gctgttcggc
     3541 catctggatg ccgctgccag cggaggacag gcggtgccgc tgccgaacat gctctatgtc
     3601 ctgccggtcg ccctagcgct ggcgggcgtc tcggcgaagc ggttgagggc ccgagaagcc
     3661 tgagccgacg ctggcgggcg ctcgtcccac gcgaagcgcc aaacgagccg gtggggtgaa
     3721 ccacgggcgg accaatgggt ccgcccgttt tatttgtcgc actctgtaat gccggcgatg
     3781 gtatcgccgg gagtggtgtg ggggccggcg ataccatcgc cggtttgaca gcgcgcggtg
     3841 ccgcgcggct tcacttgtca taattcaaaa agaaaaagat ccacgacagc gggacttcgg
     3901 ccagtggata gagcttggcg gttgacgatt tgaccgtcag cggtgccgtc ggcagcagca
     3961 gcacgtggcc gtccatgaac gcaaggttcg tgtacgcgtt cagcttgtcc gtcgtcttct
     4021 gcccgtgccg ggcggccatc tgcacgacca tcaggctcgc gttagccggt gacgacgatt
     4081 gatcggtcca gttcatgctg tcggcttcgg ccagcatgac catgttggcc gagtgtttga
     4141 tctccgtgat ctttcgcgcc agcaggggat tgctcaggcc ctgcgacagc ggcgcggtcg
     4201 tcttgttcgc ccagccgacg tacgaaacaa acggcgtcgc ccccagcccg gccgagtcgg
     4261 tctttcccgg ccactgcgtg aaggcgctga acgcgacgcg catgttcagc gcgtaccagc
     4321 tgcacaccgc gtacgccggt tgcccatcga cgcgcggaat gatcgccggc gtcacgccgt
     4381 gaccgtcggc gaagtaacca tcattggcca ggtcgtcggt cggggccttg gcgccgttgg
     4441 agtacgggct cccgttcatc agcgtgactt gatagtcctg tccttcaggg cagcgcaaga
     4501 cgctggtgcc tggcacg
//
LOCUS       DATIMH010000033         5592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_295478, whole genome shotgun sequence.
ACCESSION   DATIMH010000033 DATIMH010000000
VERSION     DATIMH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295478"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VLI90_00560"
     CDS             complement(<1..612)
                     /locus_tag="VLI90_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HSV12718.1"
                     /translation="MRDIEFIPAWYPQLRRRKRALVVQAIASGAIVLALTGVTIAKRW
                     DVQRQQAIATDSAAETARCSPALARLSALQAQQRQLRQQVQVIAQLGEQLDPTRLINL
                     LEDAMPSAVQLTDLSMETPSSDERALAIRMQGIAPDDAAVATLLTNLGGVKFFDDVTM
                     SYSRDHAGGATASREFELTFKVGVQASALQNQGLRSTSDTGVGG"
     gene            complement(605..1942)
                     /gene="pilM"
                     /locus_tag="VLI90_00565"
     CDS             complement(605..1942)
                     /gene="pilM"
                     /locus_tag="VLI90_00565"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HSV12719.1"
                     /translation="MLRLTRPQIQPIGLDIGLDSIKMLQLCVSGDGVSRALSVASAAK
                     QLLPDEARKRPEHRAALAAETIRRMFAQQPFVGRRVVAALPREIVHVKNLRLPRMTAD
                     ELPAAVETEARSLFTFDPAEACIRFLPAGEVRQGNEIRQEVIVIASRQADVDDFVQQL
                     HGSGVEIDSLDCGITGMFRAFERFVRRKDDEQDVQVLLDIGARQSQVIIAKGRDISFY
                     KQLDIGGLHLLDAVSRKLGISIEEAKALRQRLLDAGEGARRDPVRQAVYDATRNVAED
                     LGRELAMCLRYYLVTFRGQRPSRLRLVGGEAADPQLHAIFTAILSIPVEAARPLYSID
                     TMRMRTIDRQGPMSQWALALGLSLRHVAGYFSPKDGRPRVGPVVEFKADEETRGQGDE
                     TRRHGDKETRGQGDKEKEDGDEPFSAPLVPLSPCPLVSVSPLVSSNTTEASVA"
     gene            complement(2074..2652)
                     /locus_tag="VLI90_00570"
     CDS             complement(2074..2652)
                     /locus_tag="VLI90_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV12720.1"
                     /translation="MRSTRTNFAPAAVSRRRGFTFIEVLAVIVILAGMWVLVLPAMGT
                     RDDLRLGSAARVVIADLLYAQNRAISTQTTQYVNFTAASGGANGGYAIYDAQPFGAPI
                     TNPVWQQPYNVTFGAGNAAQFGTIIVTGVSLGGNPQPNTVLAFNELGQPMACTPTAAP
                     MALTATGTITLQSGDQTMVLSIEPDTGNITTP"
     gene            complement(2678..2944)
                     /locus_tag="VLI90_00575"
     CDS             complement(2678..2944)
                     /locus_tag="VLI90_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12721.1"
                     /translation="MRDPSTTTAKWLGVIVVLQVVTLAGQWAGVSIGAATPAHAQLPD
                     AGAQRNDMIAELKGVNERLDRLTAVLSSGNLQVKVAKSDDNKAR"
     gene            complement(3023..3649)
                     /locus_tag="VLI90_00580"
     CDS             complement(3023..3649)
                     /locus_tag="VLI90_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV12722.1"
                     /translation="MLHDRKQIIIEDRARPDGGSEVLQRPGLFARRTQVFGVPQTRRR
                     FRSARRAFTLVEILIVVVILAIIAAITVPQMSNASMQARENMLRDDLRFLRNQIVLYR
                     AQHHDAAPGAVANGSPSATLFVDQLTSFTDDVGNANPTMTGPFRFGPYLSKMPANPLN
                     DRRDVLLATGAALPAPDDTTGWIFNPAMPEIGVNQSGADNDGVPYSTY"
     gene            complement(3699..4139)
                     /locus_tag="VLI90_00585"
     CDS             complement(3699..4139)
                     /locus_tag="VLI90_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12723.1"
                     /translation="MRKFSDRSELGYALKELLVITVVLGGVGALVVPQFTNAGVNTRD
                     DQVNAQLRVVRAAVELYRAQHHDAAPDLSRGWTALMSPSDADGGQTQSPLFGPYLPKA
                     PVNPLTGGSTVATEPAAGIDWWWDSSTGTVTALDSNGVAYVQTQ"
     gene            complement(4228..4764)
                     /locus_tag="VLI90_00590"
     CDS             complement(4228..4764)
                     /locus_tag="VLI90_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV12724.1"
                     /translation="MTAVSTFTNRKSGIARRVVRRGFTLVEILIVVIILGILAAIVIP
                     QFTNASQDARKSNVVSQLQTLRAQIELYKLQHKDAIPKLITAGGGMWSPFTLYTDING
                     GTSATKTGNFIYGPYYQHSPVNPLAPAATGGNLGVAADGAVGWVYEEATGRICATGTD
                     STKYLVEADGSDAAAPTW"
     gene            5223..5525
                     /locus_tag="VLI90_00595"
     CDS             5223..5525
                     /locus_tag="VLI90_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12725.1"
                     /translation="MDRLSVDLPGDVSAKVRQKASRAGYASAEEYAKALLVQDAERAD
                     EADPGAPSHLSYERTHELENLLLERLEGGRSIEATPAFWDALDKQIARRQRKSKSA"
BASE COUNT          980 a         1883 c         1705 g         1024 t
ORIGIN      
        1 ccccccgacg ccggtgtctg aggtactccg aagaccctgg ttctgcagcg ccgaagcttg
       61 cacccccacc ttaaacgtca attcaaactc ccgcgaagcg gtcgcccccc ccgcgtgatc
      121 tctcgaatac gacatcgtca catcatcaaa gaatttcacg ccccccaggt tcgtcagcag
      181 cgtcgccacc gccgcgtcat cgggcgcaat cccctgcatc cggatggcca gcgcccgctc
      241 atcgctgctc ggcgtttcca tcgacagatc cgtcagctgc accgccgacg gcatcgcgtc
      301 ttccagcaag ttgatcagcc gggtcggatc gagctgctcg ccgagttgtg cgatgacctg
      361 cacctgttgc cgcagctgcc gctgctgcgc ctgcagggcg ctgagccgcg ccagcgccgg
      421 tgagcatcgc gccgtctccg ccgcggagtc cgtcgcgatc gcctgctgcc gctgcacatc
      481 ccagcgcttg gcgatcgtca cgcccgttaa cgccagcacg atcgccccgc tggcgatcgc
      541 ctggacaaca agtgcccgct tccgccgccg cagctgcgga taccacgccg ggatgaactc
      601 gatgtcacgc aacggaggcc tccgttgtgt tggaagagac aagaggagag acagagacaa
      661 ggggacaagg agacaagggg acaaggggag cggagaaggg ctcgtccccg tcctccttct
      721 ccttgtctcc ctgtcccctt gtctccttgt ctccgtgtct ccttgtctcg tctccctgtc
      781 ccctcgtctc ctcgtctgct ttgaattcaa caaccggccc cacccgcggc cgcccgtcct
      841 tcggcgagaa ataccccgcc acgtgccgca ggctcaggcc cagcgccaat gcccactgcg
      901 acatcggccc ctgccggtcg atcgtgcgca tccgcatcgt gtcgatgctg tacagcggcc
      961 gcgccgcttc aaccgggatc gaaagaatag ccgtgaagat cgcgtgcaac tgcggatcgg
     1021 ccgcctcgcc acccacgagc cgcaatcggc tcggccgctg cccgcgaaat gtcaccaggt
     1081 aatagcgcag gcacatcgcc aattcgcggc ccaagtcctc ggccacgttg cgcgtcgcgt
     1141 cgtacaccgc ctgtcgcacc gggtcgcgcc gcgcgccttc gccggcatcc agcaaccgct
     1201 gacgcagcgc tttggcttcc tcaatcgaaa tgccgagctt ccggctcacc gcgtccagca
     1261 ggtgcagccc gccgatgtcc agctgcttgt aaaagctgat gtcgcgcccc ttggcgatga
     1321 tcacctgcga ttgccgcgcg ccgatgtcca gcagcacctg cacgtcctgc tcgtcatcct
     1381 tgcggcggac gaagcgctcg aacgcgcgga acatgccggt gatgccgcaa tccagcgaat
     1441 cgatctccac gccgctgccg tgcagttgct ggacgaagtc gtccacgtcc gcctggcgcg
     1501 acgcgatgac gatcacttcc tggcgaatct cattcccctg ccgcacttcc cccgcgggta
     1561 gaaagcggat gcacgcctcg gccgggtcga acgtgaacag cgaccgcgcc tcggtctcca
     1621 ccgccgcggg caactcatcc gccgtcatcc gcggcaagcg caggttcttc acatgaacga
     1681 tctcgcgcgg caatgccgcg acaacgcgcc gaccgacaaa cggctgctgg gcgaacatcc
     1741 ggcgaatggt ctccgccgcc agcgccgccc gatgttccgg ccgcttgcgc gcctcatccg
     1801 gcaacaactg cttcgccgcc gacgccacgg aaagcgcgcg gctcaccccg tcgccggaaa
     1861 cgcaaagctg cagcatcttg atgctgtcca gcccgatatc caggccgatt ggttgaatct
     1921 gtggacgggt gagtcggagc atgtgtgcgc ggatacgtga atacgtgagt gcgtgaatac
     1981 gcgagtacgt gagcagagga tggttatgga ttacgcgtta cggattacgt tgcttctcag
     2041 gtactcacgt actcacgtac tcacgccatc ctctcacggc gtcgtaatgt tccccgtatc
     2101 gggttcaatc gacagcacca tcgtctgatc gccgctttgc agcgtgatcg ttcccgtggc
     2161 ggtcagcgcc atcggcgcgg cggtcggcgt gcaggccatc ggttggccca gttcgttaaa
     2221 cgccagcacg gtgttcggct gcggattgcc gcccaggctc acgcccgtga cgatgatcgt
     2281 gccgaactgc gccgcgttgc cggcgccgaa cgtcacgttg tacggctgct gccagaccgg
     2341 attggtgatc ggcgcgccaa acggctgggc gtcgtaaatc gcatacccgc cattcgcgcc
     2401 accgctggcg gcggtgaagt tgacgtattg cgtcgtctgc gtgctgatcg cgcggttctg
     2461 cgcgtaaagc aaatccgcga tgacgacgcg cgcggccgag cccaggcgaa gatcatcccg
     2521 cgtgcccatc gccggcagca ccagcaccca catccccgcg aggatcacga tgaccgcgag
     2581 gacctcgata aacgtaaacc cgcggcggcg cgacactgcc gcgggagcga agttggttcg
     2641 agtcgatcgc atcgtttact tctcggacgc gcgagggtta ccgggcttta ttgtcatcgg
     2701 acttcgccac cttcacctga aggttgccgc tgctgagcac cgccgtcagg cggtccaacc
     2761 gctcgttcac gcccttgagt tccgcgatca tgtcgttgcg ctgcgcgccc gcgtcgggca
     2821 gctgcgcgtg cgcgggcgtc gctgcgccga tcgacacgcc ggcccattgc cccgccagcg
     2881 tgacgacttg cagcaccacg atcacgccca gccattttgc cgttgttgtg gatggatctc
     2941 gcatgggtgt actcctctgc ttgatagcta aacgatcttc gaccgccgcc gcggctcact
     3001 ggatgagcgc gcgctggcgc cgctagtacg tggaatatgg gacgccgtcg ttgtccgcgc
     3061 cgctttggtt cacgccgatc tccggcatcg ccgggttgaa gatccaaccg gtcgtgtcat
     3121 ccggcgccgg cagcgccgcc cccgtcgcca gcagcacgtc acgccgatcg ttcagcggat
     3181 tggccggcat cttgctcagg tagggcccga agcggaacgg cccggtcatg gtggggttgg
     3241 cgttgccaac gtcgtcggtg aagctggtca attgatcgac gaacagcgtc gccgacggcg
     3301 agccgttggc caccgcgccg ggcgcggcgt cgtggtgctg ggcgcgatag aggacgatct
     3361 ggttgcggag gaatcgcagg tcgtcgcgca gcatgttctc gcgcgcctgc atgctggcgt
     3421 tggacatctg cggcaccgta atggccgcga tgatcgcgag gatcacgacg acgatcagga
     3481 tttcaacgag ggtgaaagcc cggcgtgcag aacgaaaccg acgccgggtc tgaggtactc
     3541 cgaagacctg ggtacgtcgc gcgaacagcc cgggtctttg aagtacctca gacccgccgt
     3601 cggggcgcgc acgatcttcg atgatgatct gcttccggtc gtggagcata aaactttacc
     3661 tccgcgcgcg accggacgtt ttcgtctgtg atcgcgcgct attgcgtttg aacgtacgcc
     3721 acaccgttgc tgtccagcgc ggtgaccgtg ccggtggatg aatcccacca ccagtcgatc
     3781 cccgccgcgg gttccgttgc caccgtggac ccgccggtca gcgggttcac gggcgccttc
     3841 ggtaaatacg gcccgaacag cggcgactgc gtctgcccgc cgtccgcgtc gctcggtgac
     3901 atcagcgcgg tccagccccg gctcaagtcc ggggccgcgt cgtgatgctg cgcgcgatac
     3961 aactccaccg ccgcacgcac cacgcgcaat tgtgcgttca cctggtcatc ccgcgtgttt
     4021 acgcccgcgt tggtaaactg cggcacgacc agcgcgccga ccccgccgag caccaccgtg
     4081 atcaccagca gttccttcag cgcatatccc agttccgatc gatccgaaaa cttccgcatt
     4141 cccttcccat ccttcccgct tcgatcacac gaagccggcc ggtggacgtt agcccaccgg
     4201 ccggcgagaa ctactgccac gcgggcatta ccacgtgggg gccgcggcgt cgctgccgtc
     4261 ggcttcgacc aggtacttgg tcgagtcggt accggtggcg cagatgcggc cggtcgcttc
     4321 ttcatagacc cagccaaccg cgccgtcggc ggcaacgccg agattaccac cggtcgccgc
     4381 gggggcgagc gggttcaccg gcgagtgctg gtagtagggc ccgtagatga agttgccggt
     4441 cttggtcgcg ctggtgccac cgttgatgtc ggtgtacagc gtgaacggcg accacatgcc
     4501 gccgcccgcg gtgatcagct tcggaatcgc gtccttgtgc tgcaacttgt acagctcgat
     4561 ctgggcgcgc agcgtctgca gctggctgac gacgttgctc ttgcgggcgt cctggctggc
     4621 gttcgtgaac tgcgggatca cgatcgcggc caagatgccc aggatgatca cgacgatcag
     4681 aatttcgacc agcgtgaaac cgcggcgaac cacgcgacgg gcaatgcccg acttgcgatt
     4741 cgtgaaggtg gaaacggcag tcataacggt cctccgaaaa aacgaaacac agacacagtt
     4801 cgcccgcatc acgtcgaacg gcccattgcc gcgcgaccga ccggacatgg aggaggaccg
     4861 accagaccct ttcggtaacg ttcttggtct gacgaatcag accgaacgct ttgcacacac
     4921 ggcgtgccgc acacgcgtcc cgccgcggcg ccggtcgatc gcacgcacgc atatcgcgtc
     4981 gcgtgcggtc gccggcgcgc cacctcacga tggcttcgcc cttgtcaggg gtcctccaaa
     5041 ctcacccgat gccgtctgag ttcgtgctca gtcagcgtcg gtgttattac ttatgattca
     5101 gtgcgagcgg aggcaaatga ggttttgaaa atgcggccac gatcgaaatt attggaccga
     5161 tcgtttgatt ctcgttgagc cgtgcggtga taacagataa agtcattcca ggagcacgaa
     5221 gtatggatcg actttcggtg gacttaccgg gtgatgtgag cgccaaggtg cgccagaagg
     5281 catcgcgcgc tggatacgcc agtgccgagg aatacgccaa ggcattactc gtgcaggacg
     5341 ccgagcgagc agacgaagca gatccgggcg ccccatcaca cctgtcatac gagaggacgc
     5401 acgagttgga gaatctgctg cttgagcggt tggaaggcgg ccggtcgatt gaggcgacgc
     5461 cggcgttttg ggatgcgctg gacaagcaga tcgcccgccg tcagcgaaaa agcaaatcgg
     5521 catgaaggtc cacgtcgtgc tacgcccgct ggtgccttcc gatctgctgg agatcacgtc
     5581 atatctgttc ga
//
LOCUS       DATIMH010000034         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_873030, whole genome shotgun sequence.
ACCESSION   DATIMH010000034 DATIMH010000000
VERSION     DATIMH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873030"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1104)
                     /locus_tag="VLI90_00600"
     CDS             complement(67..1104)
                     /locus_tag="VLI90_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HSV12726.1"
                     /translation="MDELRVGVIGGTGLGEALLGQTEVRQRHQIETPFGRPSDAIIEG
                     QWQGVPVLLLSRHGRGHVLNPTQVPYRANIFALKQLGCTHIIASGAVGSLREEMKPRD
                     LVIADQVIDKTSKRASTFYENAAVHVEFAEPFCPLLRQILLQASGDKAQESVKSESSA
                     SSPTAEPGPLAPYTVHDRGCYVAMEGPAFSSRAESLMHRLWGGDLIGMTAMPEAKLAR
                     EAEISYALVALVTDYDSWRHATPAAAAAKTDDAKADPLLLLEEIKANLRAASDHAIQL
                     IQRAIERMAGQREALLAAPSRQALRLAVWSEEARIAPEEIERLRPLWGRYFDNANATG
                     SPIDDAGRATA"
     gene            complement(1213..1782)
                     /locus_tag="VLI90_00605"
     CDS             complement(1213..1782)
                     /locus_tag="VLI90_00605"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HSV12727.1"
                     /translation="MRPTASKTIFTVGHSTRSLDDFIALLRAHGVRAVADVRLIPRSR
                     RFPHFNDDALAVSLPKAGIAYHPFKTLGGRRRPLRDSINMAWRNESFRGYADFMQTPA
                     FVEALRPLMELAGQTPTTTMCAEAVPWRCHRSMISDALIVRGWTVMDIMSQTSAVVHK
                     LTPFAVVRGTTITYPAVLDQETLFDGGGT"
     gene            2073..2702
                     /locus_tag="VLI90_00610"
     CDS             2073..2702
                     /locus_tag="VLI90_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HSV12728.1"
                     /translation="MAHVYPLMLDVSDRLIVIIGGGGVAARKAAGLLAAGATRVRVVA
                     PAFADEIPDDVDCIVGAYEARHLDEATLVFAATDSPEVNAAVVSEARRRGIWVNRADA
                     DEPGASDFATPALLREGAVTITVSAAGSPAIAAKLRDSIKHTLDPRWVALADAMVHLR
                     AAILASPLSGDERRELFRRLASDEAADAAHRGGVEALRGWIEQHSKLKL"
     gene            2738..3541
                     /gene="ccsA"
                     /locus_tag="VLI90_00615"
     CDS             2738..3541
                     /gene="ccsA"
                     /locus_tag="VLI90_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSV12729.1"
                     /translation="MPSNAQIFLLIVAIAFFAIGGALSYARLWRNGNSMRIGGKACLY
                     SGVVTAVAVLVWHSATRGRWLPIGDNFDALIWLAVLLALFAAYVQRRRPLAALDWFIM
                     PVVILLLAGAAVFGKTVFHPYVGRAWTSVHILTSFVGFAAFAIAAAGGALYVYAAAKL
                     RRKQPVGSYLGSLERLEHLTMTSVTLGFPLLTISIIIGTIELIAQDRPTPVTKIALAA
                     CVWVVYAIVLHAPMNPVFRGRRAAVLSVVGFILMIGALVAAQFVPGGSR"
BASE COUNT          592 a         1207 c         1113 g          652 t
ORIGIN      
        1 agcagcagca tcgaacggaa gatcggatac ggcatgatga actccagccg cttgaaggac
       61 tgctcgtcac gcggtagcgc gtccggcgtc atcgatcgga gaacccgtcg cattcgcatt
      121 atcgaaataa cgcccccaca gcgggcgcaa tcgctcgatc tcttccggcg cgatccgcgc
      181 ctcttccgac cagaccgcca gccgcagcgc ctgccgcgac ggggccgcca gcagcgcttc
      241 gcgttgcccc gccatgcgtt cgatggcgcg ctggatcagc tggatggcat gatccgacgc
      301 cgcccgcagg ttcgccttga tctcctccag cagcagcagc ggatcggctt tcgcgtcgtc
      361 cgtcttcgcg gccgcggccg ccggtgtcgc gtgccgccaa ctatcatagt ccgtcaccag
      421 cgcgaccagc gcatagctga tctccgcctc gcgcgccagt ttcgcctcgg gcatggcggt
      481 catgccgatc aggtcgccgc cccacagccg gtgcatcagg ctttccgccc ggctggaaaa
      541 cgcggggcct tccatcgcga cgtagcaccc gcggtcgtga actgtgtaag gggccagggg
      601 cccgggctca gcggtcgggg aagaagctga cgactctgac ttcactgact cctgagcttt
      661 atcccctgac gcctgcagca atatctgacg caatagcggg cagaaaggtt ccgcaaactc
      721 cacgtgcacc gcggcgtttt cgtagaaggt gctggcccgc ttgctcgtct tgtcgatcac
      781 ttgatccgcg atcaccagat cgcgcggctt catctcttca cgcagtgatc cgacggcccc
      841 cgaagcgatg atgtgcgtgc agcccagctg cttgagcgcg aagatgttcg cccgatacgg
      901 cacctgcgtc gggttcagca catgcccccg gccgtgtcgc gacagcagca gcaccggtac
      961 gccttgccat tggccttcga tgatcgcatc gctgggccgg ccgaacggcg tctcgatctg
     1021 atgccgctgg cgaacctcgg tctgccccag cagcgcttcg cccagtcccg tcccgccgat
     1081 gacgccgacg cgtagttcgt ccatggggat gaaggtaaat cacgacgcgc gaaaacgcca
     1141 cgtggtttgg gaaatgtcca cttctcgata ctctcgcccc aaaagccccg gcagagccgg
     1201 gggcttttag gattaggtcc cgcccccatc aaacaacgtt tcctgatcca gcaccgccgg
     1261 atatgtaatc gtcgtcccgc gtaccaccgc aaacggcgtc agcttgtgca ccaccgcgct
     1321 ggtctggctc atgatgtcca tcaccgtcca cccgcgcaca atgagggcat cggaaatcat
     1381 cgaccgatga caccgccacg gcaccgcttc ggcgcacatc gttgtcgtcg gcgtctgccc
     1441 ggccagctcc atcagcggcc gcagcgcctc gacaaacgcc ggcgtctgca tgaagtccgc
     1501 gtacccgcgg aaactctcgt tccgccacgc catgttgatc gaatccctga gcggccgccg
     1561 ccgcccgccc agtgttttga acggatgata cgcaattccc gctttcggca gcgagaccgc
     1621 caatgcgtcg tcattgaaat gtgggaaccg ccgcgaccgc gggatgaggc ggacgtcggc
     1681 aacggcgcgc acgccgtgcg cgcgcagcag cgcgatgaag tcgtcgagcg accgagtcga
     1741 gtggccaacg gtgaagatgg tcttggacgc ggtgggacgc atgggcgttt gaatgataag
     1801 tccgaattcc gaaacccgaa tccccgaatg aatgacgaaa tccgaatgac caaacgctgc
     1861 caatcgcgat gcttcgtcat tcggacttcg gcattcgtca ttgattcggg gattcggttt
     1921 cgccattcgg atttcctgga tcaccctaac tcactcccgc agacctgcgc tcagcagacc
     1981 tgcgctcagc agacctgcgg tcagcagtac cgggagaggg gaccggagca tattcggatt
     2041 ttcacggcgt tcccgataga atcgcccccg ctgtggcaca cgtgtatccg ctgatgttgg
     2101 acgtgagcga ccggctgatc gtcatcatcg gcggcggcgg tgtcgcggcg cgaaaagccg
     2161 ccggcctgct cgcggcgggc gcgacgcgcg tgcgcgttgt cgcgccggcg ttcgccgacg
     2221 aaattccgga cgatgtcgac tgcatcgtcg gcgcatacga ggcgcggcat ctcgatgaag
     2281 cgaccctggt tttcgccgcg accgattcgc cggaagtgaa cgccgcggtc gtttcagagg
     2341 cgcgacgtcg cggcatttgg gttaatcgcg ccgacgccga cgaaccgggc gccagcgact
     2401 tcgccacccc cgcgttgctg cgcgagggcg cggtgacgat caccgtctct gccgccggca
     2461 gccccgcgat cgcggcgaag ctgcgcgact cgattaaaca tacgctcgac ccgcgctggg
     2521 tcgcgctggc ggacgcgatg gttcacctgc gggcggcaat cctcgccagc ccattatcag
     2581 gcgacgagcg gcgcgagttg ttccgccgcc tcgccagcga cgaggcggcc gatgccgccc
     2641 accgcggtgg cgtcgaagcg cttcgcgggt ggatcgagca acattccaag ctgaaactct
     2701 aatgccgcca ccgtaacatc cccgccgaaa gccgccgatg ccttccaatg cgcaaatctt
     2761 cctgctgatc gttgccatcg ccttcttcgc catcggcgga gcattgtcgt acgcgcggct
     2821 gtggcgcaac ggcaactcga tgcgcatcgg cggaaaggcg tgcctctaca gcggcgtggt
     2881 cactgccgtc gccgtccttg tttggcacag cgcgacgcgc gggcgatggt tgccgatcgg
     2941 cgacaacttc gacgcgctca tttggctcgc cgtcctgctc gcgctgttcg cggcatacgt
     3001 gcaacgcaga aggccgctgg cggcgctgga ctggttcatc atgccggtgg tgatcctgct
     3061 gctggccggc gccgccgtgt ttggtaagac ggtgtttcac ccgtacgtcg gccgggcgtg
     3121 gaccagcgtg cacatcctga ccagcttcgt cggcttcgcc gccttcgcca tcgccgccgc
     3181 cggtggcgcg ctgtacgtgt acgcggcggc gaagctgcgg cgcaagcagc cggttggcag
     3241 ctatctcggt tcgctcgagc ggctcgagca tctgacgatg acctcggtca cgctcggctt
     3301 ccccctgctg acgatcagca tcatcatcgg cacgatcgaa ttgatcgcgc aggaccggcc
     3361 cacgccggtc acgaagatcg cgctggccgc gtgcgtgtgg gtggtttatg cgatcgtgct
     3421 gcacgccccg atgaacccgg tcttccgcgg ccgccgggcc gcggtgctgt cggtggtcgg
     3481 attcatcctg atgatcggcg cgctcgtggc ggcgcagttc gtgcccggag ggtcccgctg
     3541 atgcagcgac tgttgctgct gggc
//
LOCUS       DATIMH010000035         7662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4338577, whole genome shotgun sequence.
ACCESSION   DATIMH010000035 DATIMH010000000
VERSION     DATIMH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4338577"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..6172
                     /locus_tag="VLI90_00620"
     CDS             32..6172
                     /locus_tag="VLI90_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HSV12730.1"
                     /translation="MLTASATGLTDGSGNIVVHDGNLDHITFDVITGPKTAGTAFSAT
                     ARAFNIANEAIVTYATTGTLTAAGQAGSEPVTPSGAAFTAGVWTGSITLTVADAAVAL
                     TMTSGGIAGASSSFAVQAGAVAGFTWGAVSTPQIANVPFGQTITAKDANGNTVTSYNG
                     TVTLSGLAGTTTSQTIMGNSSSTSSSSGLLYTMGFAFTPTATAQITAVRSISGTKVSI
                     WNDTGTLLVSQAVSGTVGTWTETQLTTPITLSANTRYRIAAYMNGQTYYYRSDTPTFS
                     WGAINFSCYSSGDTFPTTTSTGEYLVDLRANVGTFTNVPITPTSATFTSGVWTGNINV
                     SQAATGMHLHVVDAASHIGDSGNFDVIQRTLTLSLPSDVREDDGTVNGLLSVSPTPNA
                     DLVISLASSDPSRLSVPATITIPAGQTSVPLPLTILDDNLLNGLEGITITATLATYAS
                     TPAVVNVHDNETAVLSVSVPASAKEGDGIVVGTVTASAAPTSDVVIQLSSGKPGRASV
                     PATVVLKAGQTTASFNISIIDNTVIDGTQFVSIGAAADNWTSGFKSISVQDNDNTITL
                     TLPASGWEGQTFSGSVAMGGTLSTDLVVNLSSLDTTELTLPATVTIPAGQKTATFTIT
                     LPTDGIKDGTQTAGVTATAQGLIAGNGSVLVHDVDLDHLGFDTITGPKSAGVAFVVTA
                     RAMNIANESIVTYTGAGTLGAFGQAGGSLTLTPTSLTFTAGVWTGNATIVAVDPAATL
                     KATVGSLSATSNSFVMQAGPVASFTWNTIGSTQTVNQPFGVTVTAKDANGFVATGYNG
                     TSTLSGQIGTTSSQTLLNGLTYTSSSSGTFTLGYAFTPNTLATVTAVRSMFGTKVSIW
                     TDTGTLLASQTVSGAAGQWTETALTTPITLQAGTRYRISEYTGGGSYFWRNDLPTMPW
                     ATDGGGYFFAGDGFPSSTSSGFYMVDLRADVGSFTSIPVTPTSATFSNGVWTGNVTVT
                     QPGNSVHLHIDDGLAHIGDSGVFNVNPTVSPIPQTPDLLAVTDSGSSTTDNITNFNNA
                     AVAKALQFSVGGTTSGATVTIYADGVAIGSTVASGATTTVTTNGTTKLTDGVRSITAR
                     QTLSGLPESLDSTALSITVDTVAPTAPVAPVLQAASDSGVSNSDGVTNQNKPTFNVAA
                     GEAGVLHLSVAGTTSFSASTTVAGAGTYPLLLSGAGTIPGGYSTTLAYSGVPAPRQVL
                     IGDINGDGKQDVVVLSAGGVITPMLYLGGGAFQALAPFTVAAGATADGLLVDVDKDNK
                     LDLVVAAGNGAAVFRGKGDGSFLAETTLGTLGAASNTIGVADFNGDGKPDIVVGTNGG
                     AGAIPLARYFGNGDGTFGAPAAMPTTSITITAVYVLGTGDFDKDGKQDVAVICAQTST
                     PNYPYEWTPLLGDGAGGARTTMGSLYTQKPTAMAIADYNNDGWLDFAIAQPLRIDIDF
                     AYTATSPMQWISGVYVATTNTPDHLSAMDVDGDGQNDIVATQGGAMFYGLSKCAPNYY
                     EFGAAQTMSVSQSASGVVSGLFTGTKRDILAVNPATNSLLMLPGNTFTALAALPDGTY
                     QATAWAEDLAGNASPISTASPITIDTAGPTVQLTAISPNPRSTPVDADTLQFNEAIAG
                     LTKGSFTLTLSGGANLLDGSQTIVGPDKINWTIGNLTGLTGAGGDYILSLTKAGITDL
                     AGNPLSAGASVQWTCNPPATQTLSIDGTSGDDTIYLNMDAAGQNLQVWINASTSGAPT
                     ATYPVSGFNAVVVNAGDGNDTVTLDLTNGAIAAANGITVNGGNGTGDSLIVQLPDSGG
                     AATVGASSINITTPAADGVGYAAVESLTITGGAGNDTVTQAAQPIASFWFNGGGNGND
                     TLNVSAGTYSCAVAQQLAALNITAPGKVLVQPSDAGALNFGAIALSGSGPLLDLANNT
                     ALLKAALSDIEAYIQNGCIMTSSAGLSVGVADIGSGKIKVMGTYSGDGNLDGTVNVGD
                     LGLLATHYGTSTANHWSTGDFDGNGVVDVADLGALATHYGQTVPTAGGGGAAALAQPA
                     SLISTSAQDPFGKKTITDQSLVDAVEALID"
     gene            complement(6250..7629)
                     /locus_tag="VLI90_00625"
     CDS             complement(6250..7629)
                     /locus_tag="VLI90_00625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HSV12731.1"
                     /translation="MRRQFSSGRWRDLRPWERAIVVVVAATIVCFGGAVVGHVAFSAR
                     HRTDLGVFLTAARAIREGRDVYQVDWHADHYLFPPLLAIALAPTLPRRGDPATIGFTL
                     TVAVTYVLSVAALLAAVHILASLLEQMVLRPLTGAAANRRWWALRATPIVVMLHSLGR
                     DLQLGQLEEFLLLALCCSLVATLNKRSFRGGLWLSIPICIKVFPAFLLIWPAARRDLR
                     FIAGAIAGCIVGVVIVPTIALGPQRAWAYGREYVETVLLQGVQRGAVAFQSGGTLSMK
                     DVHNFSLLGVFHDLQYFGQADVPAAPSPRTRAAAMSVGLALLLLTLLPALLPRRGALN
                     AADQLILFGQLIALALLIAPVFQSYYLPLLIPLVAGLTAAGMNRRPAGAIYPDRRTLL
                     LFAGFMVINTAVSVKGLEPLRDAGLPTLTVLALWTAGSAALWRRTAPARKPAARDFTL
                     SHPSPAGTV"
BASE COUNT         1446 a         2606 c         2502 g         1108 t
ORIGIN      
        1 cgatgcgctg aaggacggct cgcagacggc gatgctgacc gccagcgcga ccggtttgac
       61 cgatggcagc ggcaacatcg tcgttcacga tggaaacctc gatcacatca cgttcgatgt
      121 gatcaccggc ccgaagacgg cgggcaccgc gttcagcgca acggcccgcg cgttcaacat
      181 cgccaacgaa gcgatcgtca cctacgccac caccgggacg cttacggcgg cggggcaggc
      241 ggggagcgaa ccggtgacac cgagcggcgc ggcgttcacc gccggtgtgt ggaccggcag
      301 catcacgctg accgttgccg acgccgccgt ggcgctgacg atgaccagcg gcgggatcgc
      361 cggggcgagc agcagtttcg ccgtgcaggc gggcgcggtg gccggcttca cgtggggcgc
      421 cgtctcgacg ccgcagatcg ccaacgtccc cttcggccag acgatcaccg ccaaggacgc
      481 caacggcaac accgtcacca gctacaacgg caccgtcacg ctcagcggcc tcgccggcac
      541 gacgacgtcg cagacgatca tgggcaacag cagttccacc agcagcagct cgggcctgct
      601 gtatacgatg ggcttcgcct tcacgcccac cgccaccgcg cagatcaccg ccgtgcgcag
      661 catctccggc acgaaggttt cgatctggaa cgacaccggc acgctgctgg tgagccaggc
      721 cgtcagcggc accgtcggca cgtggaccga aacgcagctc accacgccga tcacgctgtc
      781 cgccaacacg cgctatcgca tcgccgcgta catgaacggg cagacctatt actatcgcag
      841 cgacacgccg accttctcct ggggcgcgat caacttctcc tgctattcca gcggcgacac
      901 gttcccgacc acgaccagca ccggcgaata cctcgtggac ctgcgcgcca acgtcggcac
      961 gttcaccaac gtgccgatca caccgacatc cgcgacgttc accagcggtg tgtggaccgg
     1021 caacatcaac gtgtcgcaag cggccaccgg catgcacctg cacgtcgtcg atgccgccag
     1081 ccacatcggt gacagcggga acttcgacgt gatccagcgg acgctcacgc tttcgctgcc
     1141 gtcggacgtg cgagaagacg acggaaccgt gaacggcctg ctcagcgtct cgccgacgcc
     1201 gaatgccgat ctggtcatct cgctggcgtc gagcgatcca tcgcggctga gcgtgccggc
     1261 gacgatcacg attccggccg ggcaaacctc ggtgccgctg ccgctgacga tcctggacga
     1321 caacctgctc aatgggttgg aaggcatcac gatcaccgcg acgttggcga cgtacgcctc
     1381 gacccccgcg gtggtgaacg tgcacgacaa cgaaaccgcg gtgctgtcgg ttagcgtgcc
     1441 ggcatcggca aaggaaggcg acggcatcgt cgtcggcacc gtgaccgcca gcgcggcgcc
     1501 gaccagcgat gtggtgattc agctgagctc cggcaagcca ggccgcgcga gcgtgccggc
     1561 gaccgtggtc ctcaaggcgg ggcagacgac ggcgagcttc aacatctcga tcattgacaa
     1621 cacggtgatc gacggcacgc aattcgtctc gatcggcgcg gcggccgaca actggaccag
     1681 cggcttcaag tcgatctcgg tgcaggacaa cgacaacacg atcacgctga cgctgccggc
     1741 cagcggatgg gaagggcaga cgttcagcgg ctccgtcgca atgggtggaa cgctgagcac
     1801 cgacctggtc gtgaacctct cgtcgctgga cacgaccgag ctgacgttgc cggcgacggt
     1861 gacgatcccc gcggggcaaa agaccgcgac gttcacgatc acactgccaa ccgacggcat
     1921 caaagacggc acgcagaccg cgggcgtcac cgcgacggca caggggttga tcgccggcaa
     1981 cggcagcgtg ctggtgcacg atgtcgatct ggatcacctg ggcttcgata cgatcaccgg
     2041 cccgaaatcc gcgggcgtgg ccttcgtcgt gacggcgcgg gcgatgaaca tcgccaacga
     2101 atccatcgtc acctacaccg gcgccggcac gctcggcgcc ttcgggcaag ccggcggcag
     2161 cctgacgctg accccgacgt cgctgacgtt caccgccggt gtgtggacgg gcaatgcgac
     2221 gatcgtcgcg gtggacccgg cggcgacgtt gaaggccacc gtcggcagtc tctcggccac
     2281 gagcaacagc ttcgtgatgc aggccggtcc ggtcgcaagc ttcacctgga acacgatcgg
     2341 ttcgacacag acggtgaacc aaccattcgg cgtgaccgtc accgcgaagg atgccaacgg
     2401 cttcgtcgcc accggttaca acggcacgtc gacgttgtcg gggcagattg gaacgacgag
     2461 cagccagacg ctcctgaacg gcttgaccta tacgtcaagc agcagcggga cgttcacgct
     2521 gggttatgcc ttcacgccca acacgctggc aacggtgacg gcggtgcgca gcatgtttgg
     2581 caccaaggtg tccatctgga ccgacaccgg cacgttgctc gccagccaga ccgtcagcgg
     2641 cgcggccggg caatggactg agacggcgct aaccactccg atcacgctgc aggccggcac
     2701 gcgctatcgc atcagcgagt acaccggcgg tggttcttac ttctggcgca acgacttgcc
     2761 gaccatgccc tgggcgaccg acgggggtgg atacttcttc gccggcgatg gattccccag
     2821 ttccacgtcg agcggcttct acatggtcga tctgcgcgcc gacgtcggct cgttcaccag
     2881 catcccggtc acgccgacca gcgccacctt cagcaacggc gtgtggaccg gcaacgtcac
     2941 cgtcacccag ccgggcaaca gcgtgcacct gcacatcgac gatggcttgg cccacatcgg
     3001 cgacagcggc gtgttcaacg tcaatccgac ggtgtcgccg attccgcaga cgccggattt
     3061 gctggcggtg acggattccg gcagctcgac gaccgacaac atcaccaact tcaacaacgc
     3121 cgccgtcgcc aaggcgctgc agttcagcgt cggcggcacc accagcggcg cgaccgtgac
     3181 gatctacgcc gacggcgtgg ccatcggttc caccgtcgcc agcggcgcga cgaccaccgt
     3241 gaccaccaac ggcacgacga agctgaccga cggcgtgcgc tcgatcaccg cgcggcaaac
     3301 gctgagcgga ttaccggaat cgctggactc gacggcgctg agcatcaccg tcgataccgt
     3361 tgcgccgacc gcgccggtcg cgccggtctt gcaagcggcc agcgacagcg gcgtgagcaa
     3421 cagcgatggc gtcaccaacc agaacaagcc gacgttcaac gtcgcggcag gcgaagcggg
     3481 tgtgcttcat ctgagcgtcg ccggcactac gtcgttcagc gcttcaacga cggtcgccgg
     3541 cgcgggaacc tatccgcttt tgttaagcgg cgccgggacc atcccgggcg ggtactcgac
     3601 cacgctggcg tactccggtg tgcctgcgcc gcggcaggtg ttgatcggcg atatcaacgg
     3661 cgacggcaaa caggacgtgg tcgtcctcag cgccggtggt gtgatcacgc cgatgctgta
     3721 cctgggcggc ggcgcgtttc aggcgctggc gccgttcacg gtcgcggctg gcgcgacggc
     3781 cgacggtctg ctggtcgacg tggacaagga caacaagctc gatctggtcg tcgcggcggg
     3841 caacggggcg gcggtgtttc gcggcaaggg ggacggcagt ttcctggcgg aaacgacgct
     3901 cggcacgctg ggcgcggcga gcaacacgat cggtgtcgct gactttaacg gcgacggcaa
     3961 acccgacatc gtcgtcggca ccaacggtgg ggccggcgcc attccgctgg cgcgatactt
     4021 cggcaacggt gacggcacgt tcggcgcgcc ggcggcgatg ccgacgacgt caatcaccat
     4081 cacggccgtc tacgtgctgg ggaccggtga tttcgacaag gacggcaaac aggacgtggc
     4141 ggtcatctgt gcccagacca gcacgccgaa ctatccctac gagtggacgc cgctgctcgg
     4201 cgacggcgcc ggtggcgccc ggaccaccat gggcagcctc tacacccaaa agcccaccgc
     4261 gatggccatc gcggattaca acaacgacgg ctggctggac ttcgccatcg cccagccgct
     4321 gcgcatcgac atcgacttcg cctacaccgc caccagtccg atgcaatgga tcagcggggt
     4381 gtacgtcgcc acgaccaaca cgccggatca cctgagcgcg atggacgttg atggtgacgg
     4441 gcagaacgac atcgtcgcca cgcagggcgg cgcgatgttc tacggcctga gcaagtgcgc
     4501 cccgaattat tacgagttcg gcgccgcgca gacgatgtcg gtgtcgcaat cggcatcggg
     4561 cgtggtctcg gggcttttca ccggcacgaa gcgcgacata ctcgcggtca atcccgccac
     4621 caattcgctg ttgatgctgc cgggcaacac gttcacggcg ctggctgcgc tgccggacgg
     4681 cacctaccag gccaccgcct gggccgaaga cctggccggc aacgcgtcgc ccatcagcac
     4741 ggcctcgccg atcacgatcg acacggccgg cccgacggtg cagttgacgg ccatctcgcc
     4801 gaatccgcga tcgacgccgg tggacgccga cacgctgcag ttcaacgaag cgatcgccgg
     4861 gctcaccaag ggctcgttca cgctgacgct cagcggcggg gcgaacttgc tcgatggatc
     4921 gcagacgatc gttggtcctg acaaaatcaa ctggacgatc ggcaacttga ccggactcac
     4981 cggcgcgggt ggggattaca tcttgagcct gaccaaggcc ggcattactg atctggctgg
     5041 caatccgttg tcggccgggg cgagcgtgca atggacctgc aacccgccgg ccacgcagac
     5101 gctgagcatc gacggcacca gcggcgatga cacgatctac ctgaacatgg acgcggcggg
     5161 gcagaacctg caggtgtgga tcaacgcgtc cacgagcggc gcgccgacgg ccacgtatcc
     5221 ggtgagcggc ttcaacgccg tggtcgtcaa cgccggcgac ggcaatgaca cggtgacgct
     5281 cgacctgacc aacggcgcga tcgccgccgc caacggcatc accgtcaacg gcggcaacgg
     5341 cacgggcgat tcgctgatcg tgcaactgcc cgacagcggc ggggcggcga ccgtcggcgc
     5401 gagttccatc aacatcacca cccccgccgc cgatggcgtg ggttatgccg cggttgaatc
     5461 gctgacgatt accggcgggg ccggcaacga taccgtgacg caggcggcgc agccgatcgc
     5521 cagcttctgg ttcaacggcg gcggcaacgg caatgacacg ctgaacgtca gcgccggcac
     5581 ctatagctgc gccgtcgcgc agcagctggc ggccctcaac atcaccgcgc cgggtaaggt
     5641 gctggtgcaa ccgtcagacg caggcgcgtt gaacttcggc gcgatcgcgc tgtccggcag
     5701 cgggccgctg ctcgacctgg ccaacaacac cgcgctgctg aaggcggcgc tgagcgacat
     5761 cgaagcgtac atccagaatg gctgcatcat gacctccagc gccggtctaa gcgtcggcgt
     5821 ggcagatatc ggcagcggga agatcaaagt catgggcacc tacagcggcg acgggaacct
     5881 ggacggcacc gtcaacgtcg gcgatctggg tctgctcgcc acccactacg gcaccagcac
     5941 cgccaaccac tggtccaccg gcgacttcga cggtaacggc gtcgtcgatg tcgccgactt
     6001 gggcgcgctg gcgacccact acggccaaac cgtccccacc gccggcggcg gcggggcggc
     6061 ggcactcgcg cagccggcgt cgttgatctc gaccagcgcg caggatccgt tcgggaagaa
     6121 gacgattacg gaccaaagcc tggtcgacgc ggtggaggcg ttgatcgatt gagcggcggg
     6181 ttgaaggaga agcatccggt tcgtcgcgag tacgctccga gcccggcgtg gggcgattgg
     6241 tcgagcgttt tatactgtcc ccgccggaga aggatgtgac agggtgaagt cgcgcgcggc
     6301 tggttttcgc gctggcgctg ttcttcgcca cagcgcggcg gagcccgcgg tccacagcgc
     6361 cagcaccgtc agcgttggca ggcccgcgtc gcgcagcggt tccagccctt tcacgctcac
     6421 ggcggtgttg atcaccatga agccggcgaa caacagcagc gtgcgacggt ccgggtatat
     6481 cgcgcccgcc ggtcgccggt tcattcccgc ggcagtcaac ccggccacga gtgggatcag
     6541 cagcggaagg taatacgatt gaaacaccgg cgcgatcagc agcgccagcg cgatcaattg
     6601 gccgaacagg atcaactgat cggcagcgtt tagcgcgccc cgccggggca gcagcgccgg
     6661 aagcagcgtg aggagcagca gcgcaagtcc gacggacatc gcggcggcgc gcgtgcgagg
     6721 cgacggcgcc gcgggcacgt cggcctgtcc gaaatactgg aggtcgtgga atacgcccag
     6781 cagggaaaag ttgtgcacgt ccttcatgct cagcgtgccg ccgctctgga aggcgacggc
     6841 cccgcgctgc acgccctgta gcaataccgt ctcgacgtat tcgcggccgt acgcccacgc
     6901 gcgctgcggg ccgagcgcga tcgttggcac gatcacgact ccgacgatgc acccggcgat
     6961 cgcgccggcg atgaaccgca gatctctgcg cgccgccggc cagatcagca ggaacgccgg
     7021 gaagaccttg atgcagatcg ggatcgacaa ccaaagcccg ccgcggaacg atcgcttgtt
     7081 cagtgttgca accagcgagc agcagagcgc gagcagcagg aattcctcga gctggcccag
     7141 ctgtagatcg cggccgaggc tgtgcagcat cacgacgatc ggcgtggctc gcagcgccca
     7201 ccatcgccgg ttggcggcgg cgccggtcaa tggccgaagg accatctgct ccagcaacga
     7261 tgccaggatg tgaaccgccg ccaagagggc ggcgacgctc agcacgtacg tcaccgccac
     7321 cgtcagcgtg aagccgatcg tcgccgggtc accgcgccgg ggcagcgtcg gcgccagcgc
     7381 gatcgccagc agcggcggaa acaggtaatg atccgcgtgc caatcaacct ggtaaacgtc
     7441 gcgcccctcg cgaatggccc gggcggccgt gaggaacacg ccaaggtccg tgcgatggcg
     7501 cgcgctgaac gcgacgtgtc cgacgacggc cccgccgaag cagacgatcg tcgccgcgac
     7561 gaccacgacg atcgcccgtt cccacggccg caggtcgcgc caacggccac tgctaaactg
     7621 tcgcctcatc cgctacctga acctaccgct tgcgaggaac ca
//
LOCUS       DATIMH010000036         4322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3183909, whole genome shotgun sequence.
ACCESSION   DATIMH010000036 DATIMH010000000
VERSION     DATIMH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3183909"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VLI90_00630"
     CDS             <1..648
                     /locus_tag="VLI90_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12732.1"
                     /translation="GLHLGQLDGWNVSTMLGAGYSSTHPFVHTTGIFGIGHLLFERPI
                     GERDSLVLSVDYEGNGGLFPDVPQPGFAFVHRDERLDYVLGYPLSKIAWRPMPALELS
                     ANYVVPFSADVNAEYRVAKHVGVYANAANFFQGFVVARSAQNPTGDITNRQFYQMRRV
                     ETGVRLIFDPLVDASLGIGYAFDQGFSRGFDVRDLTPFSHISNEPYLALVLRGTF"
     gene            844..2661
                     /locus_tag="VLI90_00635"
     CDS             844..2661
                     /locus_tag="VLI90_00635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV12733.1"
                     /translation="MTADRAASIEPSTPPAAISLRGRGGLIMLLVVVLGCLLPFIDKP
                     VHIDDPAYIWMAQQISHRPWDFYGFQGNYYGGVTRADVFTKNPPLMSYYLAAAAAVLG
                     WSERALHTAMFLPALLSVAGGYELGRRFSATAAGALLAGMIAAFSPVFIVSAMTITCD
                     ITMQALLIWATIFWIDGVRRGSQRRLAIGATLILAATLAKYFAACLIPLLGIYALLYW
                     RGPRRGLLWFVVPIAGLLGFELYTHHLYGGGAFTSAAEYATSARQTGGRTPVRLLSGL
                     SFVGGCMITAVPIGAAAVWHALRRRGWTGWIYAGVAAALFVGLLLWQTPEMARLAGIN
                     ALPPLVMTQLVIFALSGAAVLWLAFDELRSGEAQADSILLVLAVFGTFAFIIAFNWTV
                     SARNVLPMAPFIGVLAVRWMERARMDATTPALAPAKGWFIAVAALLPVAVLVLRADVR
                     LAESEHDAAMMVHDFAIAAGSPRVWFQGHWGFQYYMQQWGAQEVDMNSSHCAKGDLLV
                     LAWDNTNISDIPTRAVRRLARVKPLPTYWIASLHLQMMAGFHSDLYGPLPYAIGNGRA
                     ERFDIYVLEHDLVFSQSASEVPTTEPSAASSPRVIGGRR"
     gene            complement(2828..3178)
                     /locus_tag="VLI90_00640"
     CDS             complement(2828..3178)
                     /locus_tag="VLI90_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSV12734.1"
                     /translation="MKHADTTKYKGMFTPLIASRAVQAAMMVLKPGESSSENPENEHP
                     AAEQWLLVIAGSGRAKVGRRTLRLREGSLLLIERGESHQITNTGKQPMTTVNFYAPPA
                     YTKDGDVKPSVKRA"
     gene            complement(3185..4090)
                     /locus_tag="VLI90_00645"
     CDS             complement(3185..4090)
                     /locus_tag="VLI90_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HSV12735.1"
                     /translation="MATTDLSRFAIPGVLKFEPGEGGLTRAAITTPQADAHVYLHGAH
                     VTHYHPHNQAPLLFMSAKSQFAAGKPIRGGVPVCFPWFGPKKDDAAAPAHGFARLMEW
                     NVESTAKADDGSVQLVLSLGSDDATRKAWPHDFRNTFTVTIGSELRLSLEVRNTGRDA
                     FTFEEALHTYFVVGDIHKVSIDGLEATDYIDKVGGATRRNQGPQPITFTGETDRVYLN
                     TKATAVAHDVAGGRNISVAKSGSDATVVWNPWIAKAKAMRDFGDDEWPGMLCIETCNV
                     ADCAVKLDAGKAHTMTAVIRAEARR"
BASE COUNT          671 a         1408 c         1387 g          856 t
ORIGIN      
        1 ggcctgcatc tgggccaact ggatggttgg aacgtctcga cgatgctcgg cgccggttac
       61 agcagcacgc acccgttcgt gcacacgacc gggatcttcg gcatcgggca cctgctgttc
      121 gaacgcccga ttggcgagcg cgattcgctg gtcctgtcgg tcgattacga aggcaacggc
      181 ggcctgttcc ccgacgtccc gcagccgggt ttcgcgttcg tccaccgcga cgagcgcctc
      241 gattacgtgc tcggttatcc gctcagcaag atcgcctggc ggccgatgcc ggcgctggaa
      301 ctgtctgcga attacgtcgt gccgttctcg gccgacgtca atgcggaata tcgcgtcgcg
      361 aagcacgtcg gcgtttacgc caacgccgcg aatttcttcc agggcttcgt cgtcgctcgc
      421 agcgcgcaga atcccaccgg cgacatcacg aaccgccagt tctatcagat gcgcagagtc
      481 gaaacgggcg tccggctgat cttcgacccg ctggtcgacg ccagcctcgg catcggttac
      541 gcgttcgacc agggtttttc acgaggcttc gacgtgcgcg acctgacgcc gtttagtcac
      601 atatcgaatg agccgtatct cgcgctggtc ctgcgcggga cgttctgagg taaaccgcca
      661 tcctgaagcg caggttagtc gccgcagtcg aaggacctag ccaggaagcc ggtgaatcac
      721 tcctcgcgca ctgcggcgaa aggcaggcga ctgctatcgg ctccttcccc agcgcatcgg
      781 gttccattcc gcattccaag catcttcctc cgccgacgcc ctcccgatta gactcgcatc
      841 cgcatgaccg ccgatcgcgc tgcatctatc gaaccctcca cgccgcccgc ggccatctcg
      901 ctccgcggcc gcggggggtt gatcatgctt ctggttgtcg tgctgggctg cctgttgccg
      961 ttcatcgaca agccggtgca catcgacgac ccggcgtaca tctggatggc ccagcagatc
     1021 agccatcgcc cgtgggattt ttacggcttt cagggaaact actacggcgg tgtcacgcgg
     1081 gccgatgtgt tcactaaaaa cccgccgttg atgtcctatt acctggccgc cgccgcggcg
     1141 gtgctggggt ggagcgagcg cgccctgcac accgcgatgt tcctgccggc gctgctgtcg
     1201 gtcgccggcg gatacgagct tggtcgacgg ttcagcgcca ccgcggcagg ggcgctgctg
     1261 gccgggatga tcgccgcgtt ctcgccggtc ttcattgtct cggcaatgac gatcacctgc
     1321 gacatcacga tgcaggcgct gctgatctgg gcgacgatct tctggatcga cggcgtccgc
     1381 cgcggcagcc agcggcgctt ggcgattggc gcgacgctga tcctcgcggc cacgctggcg
     1441 aagtatttcg cggcgtgcct catcccgctg ctcggcatct acgcgctgct ttactggcgc
     1501 ggcccgcggc gcggattgct ttggttcgtg gtcccgatcg ccggcttgct gggctttgag
     1561 ctgtacacgc atcacctata cggcggcggg gcgttcacca gcgccgcgga atatgcgacg
     1621 tcagcgcgac agaccggcgg tcgcacgccc gtgcgcttgc tgagcggtct gtcgttcgtc
     1681 ggcgggtgca tgatcaccgc ggtgccaatc ggcgcggcag cggtatggca cgcgctgcga
     1741 cgccgcggat ggactggatg gatctacgcc ggcgttgccg cggcgctgtt cgtcggcttg
     1801 ctgctctggc agacgccgga gatggcgcga ctcgccggga tcaacgcgct gccgccgttg
     1861 gtcatgacgc agctggtcat cttcgcgcta tcgggcgcgg cggtgctttg gctggcgttc
     1921 gacgaactgc gcagcggtga ggcgcaagcg gactcgatcc tgctcgtgct ggcggtcttc
     1981 ggcacgttcg cgttcatcat cgcgttcaac tggaccgtca gtgctcgcaa cgtgcttccg
     2041 atggcgccgt tcatcggcgt gctggcggtg cgatggatgg aacgggctcg aatggatgcg
     2101 acgacgcccg cgctcgcacc ggcgaaaggc tggttcatcg ccgtcgcagc gctattaccg
     2161 gtggcggtgc tggtccttcg cgccgacgtg cggctggccg agtccgagca cgacgccgcg
     2221 atgatggtgc acgacttcgc gatcgcagcg gggtcgccca gggtctggtt ccaaggccac
     2281 tggggctttc aatattacat gcagcagtgg ggcgcgcagg aggtggacat gaactcgtcc
     2341 cattgcgcca agggcgacct gctggtgctg gcatgggaca acacgaacat ctccgacatc
     2401 ccgacgcgcg cggttcgccg gctggcccgc gtcaagccgc tgccgacgta ttggatcgcg
     2461 tcgcttcacc tgcagatgat ggcgggcttt cactcggatc tttacgggcc gctcccgtac
     2521 gcgatcggca acggacgggc cgaacgtttt gacatctacg tcctggagca cgacctggtc
     2581 ttcagtcaaa gcgccagcga ggtgcccacc acagaaccct cggcggcgtc ttcgccgcgg
     2641 gtgattggtg gacgcagatg aatttgcggc tctgtccccc tcgcccggta ctcctgaccg
     2701 caggtctctg ggagagggct ggggtgaggg taatttcgaa cgtcgagcga ccttcgcaaa
     2761 ataaaatcac cctcacccta accctctccc ggcgtaccgg gagaggggac cggagagggc
     2821 acattgtcta ggcgcgtttc accgacggct tcacgtcgcc gtccttggta tacgccggcg
     2881 gggcgtaaaa attcaccgtc gtcatcggct gctttcccgt gttcgtgatc tggtgtgatt
     2941 cgccacgctc gatgagcagc aatgagcctt cgcgcagtcg caacgtgcgc cgcccgacct
     3001 tcgctcggcc gctgccggcg atcaccagca gccattgctc ggcagcgggg tgttcgttct
     3061 ctggattctc gctcgacgat tcgcccggtt tcagcaccat catcgctgcc tgcaccgcgc
     3121 gcgacgcgat cagcggcgtg aacattcctt tgtacttggt tgtatcggcg tgcttcatcg
     3181 ccgattaccg tcgcgcttcg gccctgatca ccgcggtcat cgtgtgcgcc ttgccggcgt
     3241 cgagcttcac cgcgcaatcc gcgacgttgc acgtctcgat gcacagcatg cccggccatt
     3301 cgtcgtcgcc gaaatctcgc atcgccttgg ccttggcgat ccacgggttc cacacgaccg
     3361 tcgcgtcgga cccgctcttg gcgaccgaga tgttccgacc gccggccacg tcgtgggcga
     3421 cggcggtcgc cttggtgttg agatagacgc ggtcggtttc gccggtgaag gtgatcggct
     3481 gcgggccctg gttccggcgg gtcgcgccgc cgactttgtc gatgtagtcg gtcgcctcga
     3541 gcccgtcgat gctgaccttg tggatatcgc cgacgacgaa gtacgtgtgc aacgcttctt
     3601 caaacgtgaa ggcatcgcgg ccggtgttgc ggacctccag cgacaggcgc agttcgctgc
     3661 cgatcgttac ggtgaacgtg ttgcgaaaat cgtgcggcca ggctttgcgc gttgcgtcgt
     3721 cgctgcccag cgacagcacc aactgcacgc tgccgtcgtc cgccttggcg gtcgattcga
     3781 cgttccactc catcaagcgc gcgaagccat gcgccggcgc cgcggcatca tccttcttcg
     3841 gcccgaacca cgggaagcaa accggcaccc cgccgcggat cggtttcccc gcggcgaact
     3901 ggctcttagc gctcatgaac agcagcggcg cctgattgtg tgggtgatag tgcgtgacgt
     3961 gcgcgccgtg caggtaaaca tgcgcatcgg cctgcggcgt ggtgatggcc gcgcgcgtca
     4021 gcccgccttc accgggctcg aacttcagca cgccgggaat cgcaaagcga gagagatcgg
     4081 tggttgccat gacgtggatg ctaagagcaa gggaggcctc gattcaagcg atggcgacgc
     4141 aggacgtcag cgatcgatgc ccaacaacca tcccgcttcg cgcatcgcct gtcggaccgc
     4201 aagctgccgg tggtcattta gcgcgagcac ggtcaccgtc acgcggccca cgcttgtcag
     4261 cggaacgatc aggacgccat ccaacttgaa gtgctcgttc caggaatctc tgcggggatg
     4321 aa
//
LOCUS       DATIMH010000037         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_585431, whole genome shotgun sequence.
ACCESSION   DATIMH010000037 DATIMH010000000
VERSION     DATIMH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585431"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..624)
                     /locus_tag="VLI90_00650"
     CDS             complement(316..624)
                     /locus_tag="VLI90_00650"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HSV12736.1"
                     /translation="MTTGNASKTATTNRAKGVKGLRFEADRLIVAFRDGREVHVPLRL
                     YPTLENATLVEREAWELIGPGKVFHWPDLDLDLSVDGLIQGLRESTPAPPQRAARRSA
                     "
     gene            complement(654..866)
                     /locus_tag="VLI90_00655"
     CDS             complement(654..866)
                     /locus_tag="VLI90_00655"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSV12737.1"
                     /translation="MDYIVVIEKAADGSYSAYVPDLPGCVACGDTQDEVRQLIQEGIE
                     LHIESLREHGEPVPPPSTTTAVVHAA"
     gene            1184..1396
                     /locus_tag="VLI90_00660"
     CDS             1184..1396
                     /locus_tag="VLI90_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12738.1"
                     /translation="MAIIIENPADQQYLKQKIDEFVQSAFKQNLLALDGAFGQNLRQL
                     ASYGFNPKDLDPDEMNNLVTKVKEFL"
     gene            1621..1824
                     /locus_tag="VLI90_00665"
     CDS             1621..1824
                     /locus_tag="VLI90_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12739.1"
                     /translation="MARQLSPPERQALLDSLLAERFNAVMAESDRRRPREPTVADEQI
                     QTEVDVVRRQRRQDRQRGGSWLT"
     gene            complement(1912..2058)
                     /locus_tag="VLI90_00670"
     CDS             complement(1912..2058)
                     /locus_tag="VLI90_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12740.1"
                     /translation="MRLAVETLDADKSAHADASWSALQRLWNTSTFNSGGDRQTREQL
                     HERR"
     gene            2558..>4070
                     /locus_tag="VLI90_00675"
     CDS             2558..>4070
                     /locus_tag="VLI90_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HSV12741.1"
                     /translation="MAKTVGVGMVLVLLLMVCRSEAQPKSKAPEEHRHESAQLTGQSA
                     PPPGKLTLWYRQPAANWNEALPVGNGRLGAMVYGGVANERIQLNQDTLWEGYPQDPNN
                     PKALAALPKVRQLIFDGKSDEAAKLIGADMMGVPARIKSYQPLGDLLIDVPNLFSAEN
                     YRRDLDLTTGIATTTYKVGDTTFTREVFASYPAKAIIVHLSADQPGKVNAHLTMAREA
                     DATVEANTDVGGSSAVISLSGQLSAQYVDQAKRPWGPVKPSMRFQGLARILPTHGLIG
                     PGNGSDVFVRNADALTIEVFGATDYLRDGTLSGIDQLVTCTGHMGTIAGISYEDLRAA
                     HIKDIQNLINRVDLDLGQSPADARLLPTDERLKGLSKDPSASDPELMATYFQYGRYLL
                     ASCSRPGSMPANLQGVWNDKLNAAWNSDYHTNINIQMNYWPAEVTNLSECHQPLFDFL
                     QALSVPGAVTAKAMYNARGWVVHHLTDVYLVTTPSDGPPGVWPMGAAWMCQHLF"
BASE COUNT          808 a         1218 c         1259 g          785 t
ORIGIN      
        1 gcgcacagaa gacgtgcttg ctgaagcaac cacgccgcgc cggcgtttaa tccgccgcgc
       61 atttggtgcg ccgcgcgaat gcttctattc gtcgttcaac atcctgcaac catccatccg
      121 ggtcgtcgcc agtacctcca aacccggcgt gggggtctgc ctttcacatc atctgagcgc
      181 ggctgaccgt cgggctgcgt ggcgcgccgc aaaacgttcg cccccgtttc cgcgccaacg
      241 atgtggcgtg cagagggtgc agcgaacgcg ccggcgggat ttgcgtcgtt tgaactccat
      301 tcttagcgat cgtcgctatg cgcttcgtcg agcggcgcgt tgcggcggag ctggtgtcga
      361 ttcgcgcagg ccttggatta agccatcgac agacagatcc aaatcaaggt ccggccaatg
      421 aaacaccttg ccgggtccga tcaattccca cgcctctcgc tcgacaaggg tggcattctc
      481 aagcgttgga tacaatcgca acggcacgtg gacctcgcga ccgtcgcgga acgcgacgat
      541 caggcgatcc gcttcaaagc gcagtccctt cacgccctta gcccggttgg ttgtggcggt
      601 tttgctggcg ttgccggtgg tcatttcaat tccagagagc tgcgatgcct gtgttacgcc
      661 gcgtgcacca cggcggtcgt cgttgatggc ggcggcacgg gttcaccatg ttcccggagc
      721 gattcgatat gaagctcgat cccttcctgg atcaattgac ggacttcatc ctgcgtgtcg
      781 ccgcaggcga cgcagccggg aagatcgggc acgtaagcgg agtaactccc gtcggcggcc
      841 ttttcgatga cgactatgta gtccataagc gtctcacttc aacccagctt ggcggaggac
      901 actgttcatc gtgcccattg gaacgtcttt cgacattttc ccacccgcgg aaatcgtgac
      961 cgtgccgggc ttagatgggt gccgatactg ttgatggctg ccaagacgtg cgctggagtc
     1021 gccaaccgtc tgattcgacg agccgaacca agtcgcggta tttcatctgc ctgtccccgg
     1081 gtcggccgaa tggcaatcca tagctggctt cagcccgccc tcggcaccga tcacgcgtga
     1141 tcagcgggag cgttttcgtg ggcagctgga acgtgaactg aggttggcaa taattatcga
     1201 gaatcccgcc gaccagcagt acttaaagca gaagatcgac gagttcgtgc aatcggcgtt
     1261 taagcagaac ctactggcgc ttgatggggc gttcggccaa aatcttcgtc aattggcttc
     1321 ctacggattc aacccgaaag atcttgatcc ggatgaaatg aataatctgg taacaaaggt
     1381 taaagagttt ttgtaactgt ttaccgccga tcggttagct cgcggtgagc agtccaggct
     1441 cgttgcccgc ccagtggtcc gcgagcagat ccttaaggct ctgcatcacg tcgcggccaa
     1501 ggcaaatgcg gcgggttata tcgtcggcaa gatcgagctg atttcgcagt gaggacgacg
     1561 atagaatcct actgtcatgg ccaaactgac gatcccatta cgatcgaaca gattcgcgag
     1621 ttggcacggc agctttcgcc gccggagcgg caggcgctgc tggacagcct gctggctgag
     1681 cggttcaacg cggtgatggc ggaatccgat cgccgacgcc ctcgggagcc gacggtggcg
     1741 gacgagcaga tccagacaga agtcgacgtg gtgcgccgcc agcggcggca ggaccggcag
     1801 cgtggcggtt cttggttgac atgaacctaa tgattcgcgc gctgtttctc agcgggggtc
     1861 aaaccattcc cgagccaaaa ccgccgcagc tcaaattgct ccgtgagtgt gtcaacggcg
     1921 ttcatggagt tgctcgcgcg tctggcgatc gccgccggag ttgaaggtcg aggtgttcca
     1981 gaggcgctgg agcgccgacc aggacgcgtc ggcgtgcgcg gatttgtcgg catcgagcgt
     2041 ttcgactgcg aggcgcacgc gcgctatcag agagcgcggg ttgctcgtcg ggcttaaagg
     2101 acgccgtctt tgaatgtcgc cgccaatact tgaaccagct gatcctacct ggttgtcaaa
     2161 tcatatccga cgaacgcgcg aaaatcgcgg tcgcgcgcgt ggcgaaggtt ggctgcctcc
     2221 tgctgggcaa cgcgcccggc cctcaggggg taataagaac gtctatgttc tatgctgagt
     2281 gcgtagtcgt cgtcccgatg gctcggatcg attcgccgcg cgtgcgtgcc agcgatggga
     2341 gacggcgcga taggcgagca ccgccattgc ggatgacacc agcaggatca tcgccagcac
     2401 cgctgaacga ccgaattcga gactcggttt ttcgttggct tcggctggac atggcgggca
     2461 catccgtggc cggtgtcacc gccgaaagcg tgcaaaccgc acgcctgtcg cgtgtatgag
     2521 tcggacgtat gaagccgctc agcgtaggag attgatcatg gccaaaacgg tcggcgtcgg
     2581 aatggtgctg gttttgttgc tcatggtttg caggtctgaa gcgcagccga agagcaaagc
     2641 gccggaagaa catcgccatg agagtgctca gttgaccggg caatctgccc caccgcccgg
     2701 caagctcacc ctctggtacc gccagccggc ggccaactgg aacgaagcgc tgcccgtcgg
     2761 caacggccgg ctcggcgcga tggtgtacgg cggcgtcgca aacgaacgca tccagctcaa
     2821 ccaagacacg ctctgggaag ggtacccgca agaccccaac aacccgaagg ccctggcggc
     2881 gctgccgaag gtgcggcagc tgatcttcga cggcaaatcc gacgaagccg ccaagttgat
     2941 cggcgctgac atgatgggcg tgccggctcg catcaagtca tatcaaccgc tgggcgatct
     3001 gctgatcgac gtaccgaacc tgttttccgc cgagaattat cgccgcgatc tggacctgac
     3061 caccggcatt gcgacgacga cgtataaagt gggcgacacc acgttcacgc gcgaagtctt
     3121 cgcctcgtac cccgccaagg cgattatcgt tcatctcagc gccgaccaac cgggcaaggt
     3181 caacgcacac ctgacgatgg cgcgcgaagc ggatgccacg gttgaagcca ataccgatgt
     3241 cggtggctcg tccgccgtga tctcactgag cgggcaatta tccgcgcagt acgtcgacca
     3301 ggccaagcgg ccgtggggac cggtgaaacc gtcgatgcgc tttcaggggc tagcgcgcat
     3361 cctgccgacg catggcctga ttggaccggg caacggcagc gacgtgttcg ttcgcaacgc
     3421 tgacgcgctc accatcgagg tgttcggcgc aaccgattat ctgcgcgacg gcacgctcag
     3481 cggcatcgat caactggtca cctgcaccgg ccacatggga accatcgccg gcatctctta
     3541 cgaagacctg cgcgccgccc acatcaagga cattcagaac ctcatcaacc gcgtcgatct
     3601 cgatcttggt caaagccccg ccgacgcgcg gctgttgccg acggacgaac gcctgaaagg
     3661 cctgagcaaa gatccgagcg caagcgatcc ggaattgatg gcgacgtatt tccaatacgg
     3721 ccgctatctg ctggccagct gctctcgccc aggatcgatg ccggcgaatt tgcagggcgt
     3781 gtggaacgac aagctgaacg ccgcgtggaa cagcgactat cacacgaaca tcaacatcca
     3841 gatgaactat tggccggccg aggtgacgaa cctatccgag tgtcatcagc cgctgttcga
     3901 ttttctgcag gcgctgtcgg tgcccggcgc ggtgacggcc aaggcgatgt acaacgcgcg
     3961 aggatgggtg gtgcatcatc tgacggacgt gtatttggtg acgacgccga gcgacggccc
     4021 gccgggcgtg tggccgatgg gcgcggcgtg gatgtgccag cacctgttcc
//
LOCUS       DATIMH010000038         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_296657, whole genome shotgun sequence.
ACCESSION   DATIMH010000038 DATIMH010000000
VERSION     DATIMH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296657"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VLI90_00680"
     CDS             <1..813
                     /locus_tag="VLI90_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12742.1"
                     /translation="TVQAPQDDYFPNADPATYNSGPNPPPITDFPIPVANGTSAANYG
                     EDAIPLHGLININTASWKVLSMLPLVRATNGSVDRVNTDKLARAIVYFRDVDDGSVAG
                     TPHPHGPFKSIFELNQVVDVAGAAPPPVPPGFQNGYNNIDTRYTGRPDDPDGRQGDLV
                     PNDLRPGGAPYPTYATAAPYSPTDGVSGDFKEKFLAMTRISNLITTRSDSFTCYVLVQ
                     GWRGVGTPGAALAVQKRAAFILDRSNVGYTLDQSSGQRRLNTLPNVERVPTN"
     gene            1086..>1705
                     /locus_tag="VLI90_00685"
     CDS             1086..>1705
                     /locus_tag="VLI90_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12743.1"
                     /translation="MRSRSFRGVHRPPFTRRHLVEPLESRTLLTTAALTGTSGNDVFE
                     IAADWSAGTYQFWCGNTLQAEVPKSQGIAFTVVGNGGHDELDVDPQVSVTLTNDPGAD
                     GTKLDLRLFGYADFHDAAQLAQFQSIYPAGKGTSFTIDPDGTRLVEADSLTIDQFANG
                     ALMGRINLNDNAMIIHNPDATTAAAVQANIQAFVANSVNGGYANGA"
BASE COUNT          324 a          563 c          518 g          300 t
ORIGIN      
        1 accgttcagg cgcctcagga tgattatttc cccaacgccg atccggcaac atataactcc
       61 ggccccaacc ctccgccgat aacggatttc ccgattccag tggcgaatgg tacgtccgcc
      121 gcgaattatg gcgaagacgc gattccgctt cacgggttga tcaacatcaa cacggccagc
      181 tggaaggtgc tgtcgatgct gccgctggtc cgcgcgacca atggctctgt cgatcgagtg
      241 aacacggata aactcgcgcg ggcgatcgtg tacttccgcg acgtggacga tggcagcgtg
      301 gccggcacgc cgcatccgca cggaccattc aaatcgatct tcgagctcaa ccaagtggtc
      361 gatgtcgcag gagcagctcc accgccggtg ccgccgggtt ttcaaaacgg atacaacaac
      421 atcgacacac gctatacggg tcgccccgac gatcccgacg gacgccaagg cgaccttgtt
      481 cccaatgatc tgcgcccggg tggcgcgcca tatcccacgt atgcaaccgc ggcgccatat
      541 agccccactg acggggtctc cggcgacttc aaggagaagt tcctcgccat gacccgcatc
      601 agcaacttga tcaccacgcg gtccgattcg ttcacctgct atgtgctggt gcaaggctgg
      661 cgcggcgtgg gcacgccggg cgcggcgctg gcggtgcaga agcgggcggc gttcatcctc
      721 gaccgctcga acgtcggcta tacgctcgac caatccagcg gccaacggcg cttgaacacg
      781 ctgcccaacg tcgaacgcgt gccgacgaac tgacgccggc accggcgaac tatgaatcgg
      841 gggaaacaaa gacgcccatg gctccggagg gagccgtggg cgttcttctt ttgccgtgct
      901 tgcggaatgg cgggcatgtt ggcttgccgg gaagatgcgc gcaattcata ctgagccttg
      961 tggtgtcggg ggcgcgcatc ggcgggccgc atcattgcgt tggggctgct gcaacacaat
     1021 tcagggatgg caatatgcca ggactctcgt ctctcgggca cgccgcgcgt tgctcggtag
     1081 cggcaatgcg ttctcgctca ttccgcggcg ttcatcggcc gccgttcacg cggcggcatc
     1141 tggttgaacc gctcgagtct cgcacgttac tcaccaccgc cgcgctcacc ggcaccagtg
     1201 ggaacgacgt gttcgagatt gccgccgatt ggtcggctgg cacctaccag ttctggtgcg
     1261 gcaacacgct gcaggcggaa gtgccgaagt cgcaaggcat cgcgttcacc gtggtcggca
     1321 acgggggtca cgacgagctg gacgtcgacc cgcaggtctc cgtgaccctc accaacgacc
     1381 ccggcgccga cggcaccaag ctggatctac gcctgttcgg ctacgccgac tttcacgacg
     1441 cggcgcagct tgcgcagttt cagtcgatct atcccgccgg caagggcacg tcgtttacca
     1501 tcgatcccga cggcacgcgc ctggtggagg ccgactcact gacgatcgac cagttcgcca
     1561 acggcgcgct gatgggccgg atcaacctga acgacaacgc gatgatcatc cacaaccccg
     1621 acgcgactac cgcggcggcg gtgcaggcca acattcaggc gttcgtcgcc aactcagtca
     1681 acggcggtta cgccaacggc gcatg
//
LOCUS       DATIMH010000039         1952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4050199, whole genome shotgun sequence.
ACCESSION   DATIMH010000039 DATIMH010000000
VERSION     DATIMH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4050199"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VLI90_00690"
     CDS             <1..962
                     /locus_tag="VLI90_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12744.1"
                     /translation="LQQPCVKVQWVGFQWFYRMCGSPMPKNLNDGTNQPPRLKVYLNL
                     GTLVDLPAFSNWPRLEGAEAMAYLKAAGFEGVQDGDPQHCQSAGIGSTASGRVDAVTD
                     AEPLARRFKDLGHEAATVHVGTGFEDDAQIDALVYAVLEASDKVGFPLYIETHRATIT
                     QDPWRTIQLTRRIPDVRFNGDFSHYYTGQELVYGDIEAKWRAMQPIFGRVRFMHGRIG
                     SPGCIQVDIGDGDSAIPQVFGADFLAHFRQMWTRAMAGFLKTAQPGDYLVFAPEILAS
                     HIYYARKFTMPNGSLREESDRYAQSLVYARIARECFAAAAASL"
     gene            1139..1855
                     /locus_tag="VLI90_00695"
     CDS             1139..1855
                     /locus_tag="VLI90_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12745.1"
                     /translation="MSLMDNSFGDVQVHKHSKTMIECESCGYEMPGPMPRRCPRCFSY
                     STMREFTPNEEMLDVLITNRRRNSGPLPIRQRPSRQLATVSLRNGVFTFDLHHVAQQA
                     YLMIRAADGSARKLLPMHRIDPDIDGEENDQSFWRLTARIGSGAYHFRHYIDDGSHID
                     PIDPEEAESPSQARPEEQTLIVTASTSATLISHGSRIAVKSASSSQPMPRRRRRRRSR
                     SQMRALRQAKAAPPLPALVA"
BASE COUNT          382 a          640 c          595 g          335 t
ORIGIN      
        1 cattgcaaca accgtgcgtg aaggtgcaat gggttggctt tcaatggttc tatagaatgt
       61 gcggttcccc tatgcccaag aatctaaacg acggaacaaa ccaaccgccg cggctgaagg
      121 tttacctgaa cctgggcacg ctggtcgatc ttccggcctt ttccaactgg ccgcgcctcg
      181 aaggggccga agcgatggca tatttaaaag ccgcgggatt tgaaggcgtg caggatggcg
      241 atccgcagca ctgccaaagc gccggcatcg gatcgaccgc cagcgggcgc gtcgacgccg
      301 tcaccgatgc cgaaccgctc gctcggcggt ttaaagatct cgggcacgaa gcggccacgg
      361 ttcacgtcgg caccggtttc gaagatgacg cgcagatcga tgcgctggtg tacgcggtgc
      421 tcgaggcatc cgacaaggtt ggtttcccgc tctacatcga aacgcatcgc gcgacgatta
      481 cacaagatcc gtggcggacg atccagctga cccgccgcat tccagacgtg cgattcaacg
      541 gggacttcag ccactattac accgggcagg aactggtcta cggcgacatc gaagcgaagt
      601 ggcgggcgat gcagccgatc ttcggtcggg tgcgcttcat gcacggccgc atcggcagtc
      661 ccgggtgcat ccaggtcgac atcggcgatg gcgattctgc aatcccgcag gtgtttggcg
      721 ccgatttcct cgcccacttc cgccagatgt ggacccgcgc catggccggc tttctgaaga
      781 ccgcccagcc cggcgactat ctcgtcttcg cccccgagat cctcgcctcg cacatctact
      841 acgcgcggaa gttcacgatg ccgaacggat cgctgcgcga agagtcggat cgctacgcgc
      901 aatcgctggt gtacgcgcga atcgcccgcg agtgcttcgc cgccgcagcc gcatccttgt
      961 gaacctcgcc gccgttaccg ccgaaaaatc ggcggcacgc gctggcgcgc gggtgcggca
     1021 taaggcgaaa cccctacacc cgactcaggc atcgacttca cttccgaagt gggttggacg
     1081 ccgatatcgc gactcgcacc ggtgaatgga tactcggcgc catcaagagg agcgagcgat
     1141 gagtctcatg gacaattcat tcggcgatgt gcaggttcat aagcattcga aaacaatgat
     1201 cgaatgcgag tcgtgcggat acgagatgcc cgggccgatg ccgcggcgct gcccgcggtg
     1261 cttttcgtat agcacgatgc gcgaattcac accgaacgaa gaaatgctcg atgtgctcat
     1321 caccaatcgc cggcgcaact ccggaccgct gccgattcgc caacggccga gcagacagct
     1381 ggccaccgtg tcgctgcgca acggcgtctt caccttcgac ctgcaccacg ttgcccagca
     1441 ggcgtacctg atgatccgcg ccgccgacgg cagcgcccgc aagctgctgc cgatgcaccg
     1501 catcgatccc gacatcgacg gcgaagagaa cgaccaatcc ttttggaggt tgaccgcccg
     1561 catcgggtcc ggcgcttacc acttcaggca ttacatcgac gacggctcgc acatcgaccc
     1621 gatcgatccc gaggaagcgg aatcgccatc gcaggcgcgc ccggaggagc agacgctgat
     1681 cgtcaccgcg tcaacgtcgg ccacgctgat ctcgcacgga tcgcgcatcg ccgtgaagag
     1741 cgcgtcgtcg tcgcagccga tgccccggcg tcgccgccgt cgccgcagcc gcagccagat
     1801 gcgtgccttg cgacaagcca aggccgcccc accgctacca gcgctggtgg cgtgatgcgg
     1861 gatgtcagga aggcgcacct cgggcggcgt cggtttggat ggatcgatgg cgacggcccg
     1921 aacgtcgctg ggcgggcgct gcccatctta cc
//
LOCUS       DATIMH010000040        11228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1741148, whole genome shotgun sequence.
ACCESSION   DATIMH010000040 DATIMH010000000
VERSION     DATIMH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741148"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4614)
                     /locus_tag="VLI90_00700"
     CDS             complement(<1..4614)
                     /locus_tag="VLI90_00700"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HSV12746.1"
                     /translation="MSITAAEASPAPAAPKSPKKPNRWRRRGAIAVIALVVLGFAARI
                     LLPLTLPMVLRRIAGNYGLTCQYDRLELNLLSGDAGIWGVEFRPKEGGDAVVQADYAH
                     GNLSVLHLLRGRLDVWRVEADGVDVNVEREADGRIPLLERFISRTTTAAAPSAPSPAA
                     ASSAAPKPIDLTSPLKVEAFRLQHLRLHVRDRSLTPALDTVLAMDLRLSNLGSPTRPA
                     HFEMNVECDPMLDSLQIDGEGHSGGKTLDATINVLARGIRLKPLAAYLQPLGIRPVAD
                     GITLRASGRVRTAPTANDNGFAGALVLDKVSATADQQEALALDRLSLDATSIDNTSIR
                     LSRLALDGVRAIADRDGNGRLRVAGVEWDPSLIARPSAASPHTELGGTPTTRIESRED
                     IRVVGVPPNPAWSGKPSPLAVLLAEPWSLGEIVVTNLKTTFHDQSVTPAADLSLVSDQ
                     IAIRNIDHNPHQLDTAATLAGTLSAPGIVQEIKLSGSAKPFAAKKAFLLAVDAHGIRP
                     DALRAYLDQIGVESHLQDAAFSCSINGSVSLDADSKLRADANLETISLSDGGIDLLKF
                     PAISMAGASLDPATGRIRVDALEVSGPTTSLRREHDGALAALGLRTKRRVASASAANV
                     AAVEPAVAAAPHLPTALPLVEIGHFAWSDAHIELADDAVSPGAKVNLSDLGVDVTNFR
                     TTAAADAAPGQIRAWFAAPNIADRLSVDGTITPASNGVAVDLQIDGRKVTAAAIAPYL
                     ASLGVEPVLQKGTLAAHARAEVSQATDGLKASLSADHVRYSDGDRELAAVDALNIKDV
                     GIRAGEISVGEIDVDHARAAVARDADNSFLAAGVRVRALTSSPGTPGGGRGEGDFERR
                     TVLEISNHPSPLPEYRARGPELPFAVVLGKLHVSDSSIDWNDAAVQPSVSTTASVNLD
                     LDHLTLGRSAGPAALSLSAKVAGTIDDAKITGTVLAAPDKQAATLEISATGVRAGALA
                     AYLPPGTIVSLKDGQFHTKIDAAVSLNPAGGRRAELLVTDLNYRDGADGPTLLSLDSV
                     KLAAPRIDLPSTAIAVDELSVAGLETSAQRTSDGAMHLLGIELRSAPPAIVAATAATA
                     PSTVPAAIVSASDLVAAARQSLPLVTIAHLNLGVRRLTLSDASLPQPIVVSDLSLTNI
                     DRIEWLGKDPAGKAPTHLRLTCRIDPVMDQLTLDALVAPFAQQPSLQLDVLATGVRGD
                     GLTALAPALKANVDGSQLTDGRLAAHFETAVKLERRSPLEFDLSRGCDVDLLLKNVEY
                     RAMDKGPVLAGLEELRADAVRIEPANSLVHARTLEITKPIGFVTQDEAGIRALGWLIK
                     LPANVAATQPVTVATDAVATEPATAPAPAVAVATKPWAGDVRIDQLLISGLDARFEDH
                     SCDPPLVVPLNGLDVEVRDVSTRSLHEPGRPIRFNAIVNGGKVRLPKRGQSKVKSAEQ
                     QYEQRELFSQITVNGSLTLYPQMTGWAKTSVSGLELTALAGPAKRQNVTLRNGTYDST
                     FEARMKSDGVIETNARLIFTDLSVSEPADGPIAKLLKLPGGLDG"
     gene            complement(4611..6068)
                     /locus_tag="VLI90_00705"
     CDS             complement(4611..6068)
                     /locus_tag="VLI90_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12747.1"
                     /translation="MYNLSKRKLFPREMSLPLAAGVLLLLLIGSGCQSQRPAALPVPP
                     AIVNVTHFAGTALSGPTATAPPKTSAADALTVRVTFIALEKSPDRRFDALASRARLIS
                     STTGGSPLLSSARLTRDVQVITLQTSADAAAKLTAASAGRKSEITALTAALPPGVTAE
                     FTVRDPQLLQDSLSGVEKQRRVRIEVSRSAQANEPLQLALALDAPTGASSASSQGERA
                     IIDLAATDPHCLAMTIPFQFHDAQSRALAAVIEVSPGADDEAHQQALARCDADVRRSI
                     SSLSNQPNLLPADAERWSSLQAAIASLDIPTARRTALVFLAGRTSAHACEDVALVADE
                     ATLAKLAQQVRSSVTAVTTPQTDAAIGWTLDHAAFDFLAQLLADASASGAAPMPDELA
                     ATLAAHAGEAGRHPSSMQQVLRQVGGREDLDNRLLAENFIFLEDSSPASRVRAFDWLT
                     AHGRAPAGYDPLGTPRQRRQALEHGLSAAAAGGAP"
     gene            complement(6162..7010)
                     /locus_tag="VLI90_00710"
     CDS             complement(6162..7010)
                     /locus_tag="VLI90_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12748.1"
                     /translation="MFPLYELLSHAELSNPKPVAAAIAEARRAPRMSRYGNLTLFPCV
                     ERPEPIDDLEEEPRVLAALSNMSRNGVGLLHSDELPPGLLFDVQWETTEASIPLRFEV
                     VHSRPTSAGMYRTGARLICGELPELPMPPATRLALPAPELQNALAPEPEPIVDPEAPS
                     AIATTESEEPESLPIHGGGVLRYEPDPITDNSQPTAPAPPGTFSAAAAFGFDKTERLD
                     GVTTCGWERSIEIRRHGDRLWIYIHSPGKKNGWGIFVDADQFESALGRVQSAAASPPF
                     VTSLAA"
     gene            7631..8488
                     /locus_tag="VLI90_00715"
     CDS             7631..8488
                     /locus_tag="VLI90_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HSV12749.1"
                     /translation="MPLRLLCLGDIVGRPGRQVVHDKLPALVRDRQIDLVIANAENIA
                     GGSGITQNLFNKIRSYGVDVVTLGDHIYRKNDIIPTLQSSERVVRPANLSANAAGRAY
                     TVVTTNSGVSVAVFSLLGRIFMSHLPADDPFAAADRVLDQLPPHVRVCVCDVHAEASS
                     EKVALGHWLDGRVSLVFGTHTHIPTADAKILPGGSAYISDLGMCGPYDSVLGRRKERV
                     LKYMTTNMPAPFDVATGDVRLCGVYAEIEPETGRALNIERIEVQAGNAEQAYDADDRP
                     QQQQQPSND"
     gene            complement(8508..9791)
                     /locus_tag="VLI90_00720"
     CDS             complement(8508..9791)
                     /locus_tag="VLI90_00720"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HSV12750.1"
                     /translation="MAVMKYPRPAWLLCGVLISIPLGCSSSDSRAATNHPTTSATARS
                     DGPRVFQSPPGALADARDRYRAGDPSVAPAVNAIRAEADKALKDRPFTIVHKTHPLPG
                     VDVHDYVSIAPYFWPDTKSKEGLPYYRRDGERNPETREYDSPALSEMSSHVSTLALAG
                     YLTGDDRYAKRAAELLRVWFFDDATRMNPNLNHAQLIKGQNDGRGTGIIESNRLLPVV
                     DAIGMMQQSGAWSADDQSKIQDWFRQYVQWMRQSKNGKAEAAAGNNHGSWYDAQLATY
                     LMFLGDDAGARQLLETVKENRIARQIEPSGEMPRELARTKSFHYSVFNLSALTTLADL
                     GQRLNVDLGRYQTADGRSIRKALDWLIPFALNQKKWEHQQIIPLESHILLVPLRRAEA
                     AYHEPHYEEVVRETYRDFSTDRDNLRFPATEGPTR"
     gene            9911..>11228
                     /locus_tag="VLI90_00725"
     CDS             9911..>11228
                     /locus_tag="VLI90_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSV12751.1"
                     /translation="MTAGHAPLSPDGGEFRLGVAAFLIEELPDAVVLVDAQWRIIVAN
                     AEARRRLAIDPTPGSVTLWAVMPEAIEQSRDVLYRRAMSDRVAVRFEQHSPRDNAWLL
                     VRVAPHQQGEGLLIVLTDITNLKRSDEDLRDREDRYRLLFDRNPQPMYAYDLDTGRIV
                     IVNDSMASRYGYARGELLQMTVFDLHPPEEVPKLRELIARIGRSAQSQSGVWHHRRRD
                     GSIIAVDITSHSLMMSGRYVRVVVAIDVTDRLHAEEAMRASESLYRTTGKAMPLLMWS
                     MRADGAFEQVNPRFTEVTGKTAEDVSRQGWGSFVHPDDMPTLVELWQSQRAQAETHEM
                     ELRYRTTDGSYRWFLARAAPVTDTAGAVVRWVGVATDVDDLKRTQAELQQAKDAAEAA
                     NVAKDQFLAVLSHELRTPLTPVLLTASSLRQEPGLDPEIRDALGMIT"
BASE COUNT         1860 a         3671 c         3763 g         1934 t
ORIGIN      
        1 accatccagt ccacccggca gcttcagcag ctttgcgatc ggcccatcgg ccggttccga
       61 cacggacaga tcggtgaaaa tcagccgcgc gttggtttcg atcacgccat cgctcttcat
      121 ccgcgcttcg aacgtgctgt cgtaggtgcc gttgcgcagc gtgacgttct gccgcttggc
      181 cggccccgcc agcgcagtca gttccaagcc gctgaccgac gtcttcgccc agccggtcat
      241 ttgcggataa agcgtgaggc tgccgttcac cgtgatctgc gaaaacagct cgcgctgttc
      301 gtactgctgc tccgccgact tcaccttcga ctgcccgcgc ttcggcagcc gcaccttccc
      361 gccgttcacg atcgcgttga accggatggg ccggccgggc tcgtgcagcg atcgcgtgct
      421 gacgtcgcgg acctccacgt ccagcccatt caacggcacg accagcggcg gatcgcacga
      481 atgatcttca aaccgcgcat cgagcccgct gatcagcagc tgatcgatgc gaacatcgcc
      541 ggcccacggc ttggtcgcga ccgcgaccgc aggcgcgggc gccgtcgccg gctctgtcgc
      601 caccgcatcg gtcgcgaccg tcaccggctg ggtcgccgcg acattcgccg gcagcttgat
      661 caaccaaccc agcgcgcgaa tgcccgcctc gtcctgcgtg acgaatccga tcggcttggt
      721 gatctccagc gtgcgcgcgt gcaccagcga attcgccggc tcgatgcgca ccgcatcggc
      781 gcgcaattct tccagccccg ccaacaccgg ccccttgtcc atcgcgcgat actcgacgtt
      841 cttcagcagc agatccacat cgcagccgcg cgacaggtcg aactcgagcg gacttcgccg
      901 ttccagcttc accgcggtct cgaaatgggc ggccagtcgg ccatcggtca gctgcgaacc
      961 atccacgttc gccttgagcg ccggcgccag cgccgtcagc ccatcgccgc gcacaccggt
     1021 cgccagcaca tccagctgaa ggcttggctg ctgcgcaaac ggcgcgacca gcgcgtccag
     1081 cgtgagctga tccatcaccg ggtcgatccg gcacgtcagc cgaaggtgcg tcggcgcctt
     1141 gccggcgggg tctttaccga gccattcgat tcgatcgatg ttcgtgagcg acaagtccga
     1201 cacgacgatc ggctgcggca gggaggcatc ggacaacgtc aaccgccgca cgccgaggtt
     1261 caggtgagcg atagtcacca gcggcaatga ttgccgcgcc gcggcaacta gatcgctggc
     1321 gctgacgatt gccgcgggca cagtgctcgg cgcagtagcc gcagtcgctg caacgatggc
     1381 cggcggcgcc gatcgcaatt cgatccccag cagatgcatc gcgccgtccg acgtgcgctg
     1441 cgcgctggtt tccaagccgg cgaccgacag ttcgtcaacg gcaatcgcgg tgctcggcag
     1501 atcgatccgc ggcgccgcca gcttcaccga atccagcgac aacagtgtcg gcccatcggc
     1561 gccgtcgcga tagttcaggt ccgtgacgag caactccgcc cgccgtccac cggcgggatt
     1621 gagcgaaacc gccgcgtcga tcttcgtgtg gaactgcccg tccttcagcg agacgatggt
     1681 ccccggcggc agatacgccg ccagtgcgcc ggctcgaacg ccggtcgccg agatctccag
     1741 cgtcgccgcc tgcttgtccg gcgcggccag caccgtgcca gtgatcttcg cgtcatcgat
     1801 cgtcccggcg actttggcgc tcagcgacaa cgccgcgggc cctgcagatc gtccaagggt
     1861 cagatgatcg agatcgagat tgaccgatgc agtcgtgctg acactcggct gcaccgcggc
     1921 gtcgttccaa tcgatcgacg agtcactcac gtgcagcttc cccagcacca cggcgaacgg
     1981 cagttccggt cccctcgccc ggtactccgg gagagggcta gggtgatttg aaatttcgag
     2041 aactgttcga cgttcgaaat ccccctcacc ccggccccct cccggcgtac cgggcgagga
     2101 ggtcagagcg cgcacccgca cgcccgcggc gaggaaggag ttgtcggcgt cgcgcgcgac
     2161 tgcggcgcga gcgtgatcga cgtcgatctc accgacgctg atttcgccag cgcggatgcc
     2221 cacgtctttg atgttcagcg catcgaccgc cgcgagttcg cggtcaccgt cgctgtagcg
     2281 cacgtgatcc gccgacagcg acgccttcag gccatcggtc gcctgcgaga cttccgctcg
     2341 cgcgtgcgcc gcgagcgtgc ccttctgcag caccggctcg acgccgaggc tcgccagata
     2401 cggcgcgatc gccgccgcgg tgactttgcg gccatcgatt tgcagatcga ccgccacccc
     2461 gttgcttgcc ggcgtgatcg tgccatcgac gctcaatcgg tccgcgatgt tcggcgccgc
     2521 aaaccaggcg cggatttgcc ctggcgccgc gtcggcagcg gcggtcgtgc ggaagttggt
     2581 cacgtcgacg cccaggtccg acaggttcac tttcgcgccg ggcgagacgg cgtcgtcggc
     2641 caattcgata tgcgcatcgc tccacgcgaa gtgcccgatc tccaccagcg gcaacgccgt
     2701 cggcagatgg ggggcggcgg caacggccgg ctcgacagcg gcaacgttgg cggcagaagc
     2761 actcgcgacg cgccgcttcg tccgcaggcc cagcgccgcc agcgcgccgt catgttcccg
     2821 gcgcagcgat gtcgttgggc cgctgacttc tagcgcgtcc acgcgaatgc gcccggtcgc
     2881 cgggtcgagg gacgccccgg ccatgctgat cgcggggaac ttcagcaggt cgattccgcc
     2941 gtcgctcagt gagatcgttt cgaggttcgc gtccgcccgc agcttgctgt cggcatcgag
     3001 cgaaaccgag ccgttgatcg agcagctgaa cgcggcatcc tgcagatgcg attcgacgcc
     3061 gatctggtcg aggtacgccc gcagcgcatc cgggcgaatg ccgtgcgcat ccactgccag
     3121 cagaaacgcc ttcttcgccg cgaagggttt ggcgctgccg gacagcttga tttcctgcac
     3181 gatgcccggc gcgctcagcg tgccggccaa tgttgcggcc gtgtccagct gatgcgggtt
     3241 gtgatcaatg ttgcggatcg cgatctgatc cgacaccagc gacaaatctg ccgccggtgt
     3301 gaccgattga tcgtggaacg tcgtcttcag attcgtgacg acgatctcgc ccaggctcca
     3361 cggttcggcc agcagcaccg cgagcggtga cggcttgccc gaccacgcgg ggttcggagg
     3421 tacgccgacg acccggatgt cttctcgcga ctcgatccgg gtcgtcggcg tacctccgag
     3481 ctcggtgtgg ggggatgcag cactggggcg cgcgatcaag ctcgggtccc actcgacgcc
     3541 ggcgacgcgc aggcggccat tgccatcgcg gtcggcgatc gcgcgcacgc catccagcgc
     3601 caggcgcgaa aggcgaatcg aggtattgtc gatcgacgtc gcatccagcg acagacggtc
     3661 cagcgccagc gcttcctgtt gatcggccgt ggccgagact ttgtcgagca ccagcgcccc
     3721 ggcaaaacca ttgtcattcg cggtcggcgc cgtgcgcacg cgtccgctgg cgcgcagcgt
     3781 gatgccatcg gccacggggc gaatgccaag cggttgcaaa tacgcggcga gcggcttcag
     3841 tcggatgccg cgggccagga cattgatcgt cgcgtcgagc gtcttgccgc cgctgtggcc
     3901 ttcgccgtcg atctgcagcg aatcgagcat cgggtcgcac tcgacgttca tctcaaagtg
     3961 cgccggccgg gtgggcgagc cgaggttcga cagccgaagg tccatcgcca ggacggtgtc
     4021 cagcgccggc gtgaggctgc ggtcgcgcac gtgcaggcgc aagtgctgca ggcgaaacgc
     4081 ttccaccttc aacgggctgg tgaggtcgat cggtttcggc gccgcgctcg atgccgcggc
     4141 gggagatggc gcgctcggcg cggccgcagt cgtcgtgcga ctgatgaatc gttccagcag
     4201 cgggatgcgg ccgtcggctt cgcgctcgac gttcacatcg acgccgtcgg cttcgacgcg
     4261 ccatacgtcc agccggccgc gcagcaggtg caggaccgag aggttgccgt gggcgtagtc
     4321 ggcttgaacg accgcgtcgc cgccttcctt ggggcgaaac tcgacgcccc agatgccggc
     4381 gtcgccgctg agcaggttca gctccaggcg atcgtattgg cacgtgagac cgtagttgcc
     4441 ggcgatccgt cgcagcacca tcggcaacgt cagcggcaac aggatgcgcg cggcaaagcc
     4501 gagcacgacc agcgcgataa ccgcaatcgc accgcggcgg cgccagcggt tgggtttttt
     4561 cggcgacttc ggagcggcgg gagccggcga agcttcggca gcggtaatac tcacggcgca
     4621 cctccagcag cagcggcaga caggccatgc tccagcgcct gtcggcgttg tctcggcgtg
     4681 ccgagcgggt cgtaccccgc gggggctcgg ccgtgtgcgg tcaaccagtc gaacgcccgc
     4741 acgcgcgacg cgggcgaact gtcttcaagg aagatgaaat tctcggccag caggcgattg
     4801 tccagatcct cgcggccgcc gacctggcgc agcacctgct gcatcgatga tggatgccgc
     4861 cccgcttcac cagcgtgtgc ggccagcgtc gcagccaatt catcgggcat cggtgccgcg
     4921 ccggaggcgc tggcgtcggc aagcaactgg gcgaggaagt cgaacgccgc gtgatccagc
     4981 gtccagccga tggccgcgtc ggtttgcggc gtcgtcaccg cggtgaccga cgatcgaact
     5041 tgttgcgcca gctttgccag tgtcgcctcg tccgcgacga gcgcgacatc ttcacaagcg
     5101 tgcgcgctgg ttcgaccggc gaggaaaacc aacgccgtgc ggcgagccgt ggggatatcc
     5161 agcgatgcga tggcggcctg caggctggac cagcgctcgg cgtcggccgg caacaggttg
     5221 ggctggttcg acagcgacga gatcgagcgg cggacgtcgg cgtcgcatcg cgccaacgcc
     5281 tgctggtgcg cttcgtcatc ggcgcccggc gagacttcaa tgacggcggc gagcgcgcgg
     5341 ctttgggcgt cgtgaaactg gaacgggatc gtcatcgcca ggcagtgcgg atcggtggcg
     5401 gcgaggtcga tgatcgcgcg ttctccctgc gacgatgccg acgaggcgcc cgtcggggca
     5461 tccagcgcca gggccaattg caatggctca ttcgcctgtg cggatcgcga tacttcaatt
     5521 cgcacgcgcc gctgtttctc cacgcccgac agcgaatctt gcagcagttg cggatctcga
     5581 acggtgaatt ccgccgtgac gcccggcggc aatgccgcgg tgagcgcggt gatctccgac
     5641 ttccgaccgg cgctggcggc ggttagtttt gccgcggcat cggcgcttgt ttgcaacgtg
     5701 atgacctgca cgtcacgcgt gaggcgggcg gacgacagca gcggcgagcc gccggtggtg
     5761 gatgagatca atcgcgcgcg gctggcgagg gcatcgaagc gacggtcggg cgatttctcc
     5821 agcgcgataa acgtgacgcg cacggtcagc gcgtcggcgg cggacgtttt aggcggcgcg
     5881 gtggcggtcg gcccggacag cgcggtgccg gcgaagtggg tgacgttgac gatggccggt
     5941 ggaacgggca acgcggcggg gcgctggctc tggcagccgc tgccgatcag cagcagcagc
     6001 aacacccccg cggccagcgg cagcgacatc tcgcgcggaa acaacttgcg cttcgaaagg
     6061 ttgtacacgg atcggcccta catcctgcgg caccagtgtc tgccgcggca acatcttggc
     6121 agacgaatcg attcaacgca ataaaactta agttgcggcg atcaagcggc cagcgaggtg
     6181 acgaacggcg gcgacgccgc ggccgactgc acgcgcccca gtgcggattc gaattgatcc
     6241 gcatcgacga agatgcccca gccgtttttc tttcccggcg aatggatgta gatccacaag
     6301 cgatcgccgt ggcggcggat ctcgatcgag cgttcccacc cgcaggtggt gacgccgtcg
     6361 aggcgctcgg tcttgtcaaa gccaaacgcg gcggcggcgc tgaaggtgcc cggcggcgcg
     6421 ggggcggtgg gttggctgtt gtcggtgatt ggatccggct cgtagcgcag gacaccgccg
     6481 ccgtggatgg gcagtgattc gggttcttcc gattccgttg ttgcgatcgc gctcggcgct
     6541 tccgggtcga cgatcggttc cggctctggc gcgagcgcgt tctggagttc gggcgcgggc
     6601 aaggccagcc gcgtggccgg cggcattggc agctcgggca gctcgccgca gatcagccgc
     6661 gcgccggtgc gatacatgcc agccgacgtc ggccggctgt ggacgacctc gaatcgcagc
     6721 gggatcgacg cttcggttgt ttcccattgc acgtcgaaca acaggcccgg cggcagttcg
     6781 tccgagtgca gcaggccgac gccgttgcgc gacatgttgc tcagcgccgc cagcacgcgc
     6841 ggctcttctt ccaggtcgtc gatcggctcc ggccgctcaa cgcaggggaa cagcgtgagg
     6901 ttcccataac gcgacatccg cggagcgcga cgtgcttcgg cgatcgcggc ggcaacgggt
     6961 ttagggttcg ataactcggc gtgactgagt aattcgtaca gcgggaacat gtcgttaaaa
     7021 tcgtcacgga cggcaggggc gttaaggtta gattctcttt ttatttcgtg gtggttggag
     7081 agtgacgccg ttgttacgct tgcacggccc ctcaccccag ccctctcccc atgcgtagat
     7141 ggggataggg agccggagag cgcgcgatgc gacgccagag aattcgcgcg gccaatctgt
     7201 tctatgcgcg ctcgatgcgc accgaaccga cccgcgctga aggaatcttg tgggaacacc
     7261 tgcgcgaccg gcgcctgggc gggttcaagt tccggcggca gtacggactc gatggtttca
     7321 tcctcgactt ctactgcgct caggcaaagc tggtcgtcga gttggacggt gcgtcgcacg
     7381 tcggaaaaga aaattacgat cgggatcgaa cgatctggtt ggaagtgtgc ggtttgaacg
     7441 tcgtgcgcta taccaatgac gctgtcttca acgctctgga cgaagtaaag tcgtccatcc
     7501 tcgaacactg caaccgccgc gcccaccacc ctctccccgg tacgccgggg agagggccag
     7561 ggtgcggggc aagccggcag tcgaacggca caccttcgcc gaagtgtcgc caagcgttat
     7621 catcatgcca atgcccctcc gcctcctctg tctcggcgat atcgtcggcc gccccggccg
     7681 ccaagttgtg cacgacaagc tccccgcgct cgtgcgcgat cgacagatcg acctggtcat
     7741 cgccaacgcc gagaacatcg ccggcggcag cgggatcacg cagaatttgt tcaacaagat
     7801 ccgctcgtat ggcgtcgacg tcgtcacgct gggcgatcac atctatcgca agaacgacat
     7861 catccccacg ctgcaaagca gcgaacgcgt tgttcgcccg gcgaacctgt cggctaacgc
     7921 cgcggggcgc gcttacaccg tggtcacgac caacagcggt gtttccgtcg ccgtcttttc
     7981 gctgcttggc cggatcttca tgagccatct gccggcggat gatccgttcg cggcggccga
     8041 tcgcgtgctg gaccagctgc cgccgcacgt gcgcgtgtgc gtgtgcgacg tgcacgccga
     8101 ggcgtcgagc gaaaaggtgg cgttgggtca ctggctggat ggccgggtaa gcctcgtctt
     8161 cggcacccat acgcacatcc cgacggcgga cgcgaagatc ctccccggcg gcagcgctta
     8221 catcagcgac ctgggcatgt gcggcccgta cgattcggtc ctgggccgcc gcaaggaacg
     8281 cgtactgaaa tacatgacga cgaacatgcc cgcgccattc gacgtcgcga ccggcgacgt
     8341 ccgcctgtgc ggcgtgtacg ccgagatcga acccgagacg ggccgcgcgc tcaacatcga
     8401 gcgcatcgaa gtgcaggcgg ggaatgccga gcaggcgtat gacgcggacg atcgcccgca
     8461 acagcagcaa caacccagca acgactaaca caccggccgc actcgcttca gcgggtcggt
     8521 ccctcggtcg ccgggaatcg cagattgtcc cgatcggttg agaagtcgcg atacgtttcg
     8581 cgcacgacct cttcgtaatg cggctcgtga tacgccgctt ccgcccggcg tagcggcacc
     8641 agcaggatgt gtgattcgag cgggatgatc tgctggtgct cccacttctt ctggttcagc
     8701 gcgaagggga tcagccagtc gagcgctttg cggatcgacc ggccgtcggc cgtttggtaa
     8761 cgccccaggt caacattcag ccgctggccg agatcggcca gcgttgtcag cgctgacaag
     8821 ttgaagacgc tgtagtggaa cgacttggtc cgcgccagct ctcgaggcat ttcgccgctg
     8881 ggttcaatct gccgcgcgat tcgattttcc tttaccgtct ccagcagctg ccgcgcgccg
     8941 gcgtcgtcgc cgaggaacat caggtacgtc gccagctgcg cgtcgtacca cgaaccgtgg
     9001 ttgtttccgg ccgcggcctc ggctttgccg ttcttgctct ggcgcatcca ctgcacgtac
     9061 tggcggaacc aatcctggat cttcgattga tcgtcggcgg accacgcgcc gctctgctgc
     9121 atcatgccga tcgcatcgac cacgggcagc aggcggttcg attcgatgat gccagtgccg
     9181 cggccgtcgt tctggccttt aatcaattgt gcgtgattca ggttcgggtt catgcgcgtc
     9241 gcatcgtcga agaaccagac gcgcagcaac tcggcggctc gcttggcgta tcggtcatcg
     9301 ccggtgagat agccggccag tgccagcgtg gagacgtggc tcgacatctc cgacagcgcg
     9361 ggcgaatcgt actcgcgcgt ctcgggattg cgctcgccgt cgcgccggta atacggcagg
     9421 ccctccttgg atttggtgtc cggccaaaag tacggtgcga tgctgacgta gtcgtgcacg
     9481 tccacgccgg gcagcggatg ggtcttgtgg acgatcgtga acggccggtc cttcagcgcc
     9541 ttgtcggcct ccgcgcgaat cgcgttcacc gcaggcgcga ccgacggatc accggcgcga
     9601 tatcgatcgc gggcatccgc cagcgccccc ggcggcgatt gaaacacccg cgggccgtcc
     9661 gagcgcgcgg tcgcggacgt cgttggatga ttcgtcgcag cgcgcgaatc gctgctggaa
     9721 cagcccagtg ggatggagat caggacgccg caaagcagcc aggcgggacg cgggtacttc
     9781 atcaccgcca ttaccggcct ttggtcgcgg cgttgaagtg ccggccgctg gcgcatcagc
     9841 ataaagcccg gtaggagaga tcgccgcgtt gcggtatcgt attgggtcgt tcgccgcagt
     9901 tggtttgacc atgaccgcag ggcatgcacc tctttcgccc gatggcgggg aattccgcct
     9961 gggggttgct gcattcctca tcgaagaact gccggatgcc gttgtgctgg tcgatgcgca
    10021 gtggcggatc atcgtggcca acgccgaggc gcgccggcgg ctggccatcg atccgacgcc
    10081 gggcagcgtg acgctttggg cggtgatgcc cgaggcgatc gagcaatcgc gcgacgtgct
    10141 gtatcgccgg gcgatgagcg atcgcgtcgc cgtgcgcttc gagcagcact cgccgcgcga
    10201 taacgcgtgg ctgctggttc gggtcgcgcc gcaccagcaa ggggaggggc tgctgatcgt
    10261 cctgaccgac atcacgaacc tcaagcgcag cgatgaagac ctgcgcgacc gcgaggaccg
    10321 ctatcgcctg ctgttcgatc gcaacccgca gccgatgtac gcgtatgacc tggacaccgg
    10381 gcgcatcgtg atcgtcaacg attcgatggc gtcgcgttac ggctacgcgc gcggcgagct
    10441 gttgcagatg acggtatttg acctgcaccc gccggaggag gtgccgaagc tgcgcgagtt
    10501 gattgcgagg atcgggcgaa gcgcgcaatc gcagtcgggc gtgtggcacc accggcggcg
    10561 cgacgggtcg atcatcgcgg tggatatcac cagccatagc ctgatgatgt ccgggcgata
    10621 cgtgcgcgtt gtggtggcga tcgacgtgac cgatcgcctg cacgccgaag aggcgatgcg
    10681 cgccagcgaa agcctgtatc gcacgaccgg caaggcgatg ccgctgctga tgtggtcgat
    10741 gcgggcggac ggcgcgttcg agcaggtgaa cccgcgcttc acggaggtga ccggcaagac
    10801 cgcggaggat gtgagccgcc aaggctgggg aagttttgtt caccccgacg atatgccgac
    10861 gctggtcgag ctgtggcaga gccagcgcgc gcaggccgag acgcacgaga tggagctgcg
    10921 ctatcgcacc accgatgggt cctatcgatg gttcctggcg cgcgcggcgc cggtgacgga
    10981 caccgccggg gcagtcgtgc gatgggtcgg cgtggcgacg gatgtggatg acctgaagcg
    11041 cacgcaggcg gagcttcaac aagccaagga cgcggccgag gcggcgaacg tggccaagga
    11101 ccagttcctg gcggtgctga gccacgagct gcgcacgccg ctgacacccg tgttgttgac
    11161 ggcatcttcg ctgcggcagg agccgggttt ggatccggag attcgcgatg cgctgggcat
    11221 gatcaccg
//
LOCUS       DATIMH010000041         1568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_874740, whole genome shotgun sequence.
ACCESSION   DATIMH010000041 DATIMH010000000
VERSION     DATIMH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874740"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VLI90_00730"
     CDS             complement(<1..916)
                     /locus_tag="VLI90_00730"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HSV12752.1"
                     /translation="MPSRPGQSAAMHGVRARPAIKKIPTPRMQVFRGFNHPGIAPACA
                     LTIGNFDGVHRGHQAMLALLNNEAQHRGVPSCVLTFEPHPRDFFAALARKPEMAPARI
                     ATLRDKLTELARCGVQQCVVLPFDTRLSSQVPEAFVDNVLVRGLGVKYVLVGDDFRFG
                     AKRAGDYAMLDAAGARLGFDVARMNSYEVHGVRVSSSEVRSALARADMDGVAALLGRP
                     YSISGHVVHGRKLGRQLGFKTLNLRFSHWKPAASGIFAVRVLGLHDGERLPDGSHKPV
                     IGVANLGIRPSVDPSDVNGGRVLLETHCL"
     gene            1008..1559
                     /locus_tag="VLI90_00735"
     CDS             1008..1559
                     /locus_tag="VLI90_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSV12753.1"
                     /translation="MLKLSAQGLPQSWISLEQAVLHYAADEVRWEVGARVALFRGGHN
                     ALSGKQSQIAVSSIIGTKGVPNINPFDLKPGLTNVKLFARDRNMCAYCGDLFHEDELT
                     REHIIPFAQNGRDHWMNVVTACRACNHRKSNRTPEQARMPLLYTPYVPSLWEDFILRN
                     RRILADQMEFLMAHLPKNSRLHA"
BASE COUNT          298 a          509 c          502 g          259 t
ORIGIN      
        1 cgaggcaatg ggtctcgagc agcacgcgcc cgccgttgac gtcgctcgga tcgaccgacg
       61 gccggatccc caggttggcg acaccgatca cgggcttgtg cgatccatcg ggcaaccgct
      121 cgccatcgtg caagccaagg acgcgcaccg cgaagatccc gctggccgcc ggcttccagt
      181 gcgaaaagcg caggttcagc gtcttgaagc cgagctgccg gcccaacttg cggccgtgca
      241 ccacgtgacc gctgatgctg tagggccggc cgagcagcgc cgccacgccg tccatgtcag
      301 cgcgtgccag cgcggaacgc acttcggagc tggagacgcg gaccccgtgc acctcgtagc
      361 tgttcatgcg cgcgacgtcg aagcccagac gcgcacccgc ggcatcgagc atcgcgtaat
      421 cgccggctcg cttcgcgccg aaccggaagt cgtctcccac cagcacgtac ttcacgccca
      481 ggccgcgcac caggacgttg tcgacgaacg cttcgggaac ttgggacgag agacgggtgt
      541 cgaacggaag taccacgcac tgctgcacgc cgcagcgcgc gagttcggtg agtttgtcgc
      601 gcaaggtggc gatgcgcgcg ggcgccatct ccggcttgcg cgccagcgcc gcgaagaaat
      661 cccggggatg gggctcgaag gtcagcacgc agctggggac accgcggtgc tgcgcttcgt
      721 tgttcagcag cgccagcatc gcctggtggc cgcggtgcac gccatcgaaa ttcccgatgg
      781 tcagcgcgca cgcgggtgcg atgccgggat ggttgaatcc tctgaaaact tgcatgcgcg
      841 gcgtcggtat ctttttgata gcaggtcgag cccgcacgcc gtgcatcgca gccgattgtc
      901 cagggcgaga gggcatgaaa cagggtatat tgtgctgtac agcgccacag tgcgttgtac
      961 cgaatcttca agtgatcccg gacgatcgaa ggagtcgtgt tttgaaggtg ctgaagctgt
     1021 ccgcgcaagg cctgccgcag tcgtggattt cgctcgaaca ggccgtgctc cactacgccg
     1081 ccgatgaggt gcgctgggaa gtgggcgcgc gggtggcgct gtttcgcgga gggcataacg
     1141 cgctcagtgg caagcaatcg cagatcgccg tcagcagcat catcggcacc aagggcgtgc
     1201 ccaacatcaa tcccttcgat ctcaagccgg gactcacgaa cgtcaagctg tttgcacgcg
     1261 accgcaatat gtgcgcctac tgcggcgacc tgttccacga agacgagctc acacgcgagc
     1321 acatcattcc gttcgcacag aacggccgcg atcactggat gaacgtggtc accgcctgcc
     1381 gcgcttgtaa ccatcgcaag agcaaccgca cgcccgagca ggcgcgcatg ccactgctgt
     1441 acacgccgta tgtcccgagc ctatgggaag acttcatctt gcgcaaccgc cggatcctgg
     1501 ccgaccagat ggaattcctg atggcgcacc tgccgaagaa ctcacggctg cacgcctgag
     1561 ctgaagtg
//
LOCUS       DATIMH010000042         5000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_586307, whole genome shotgun sequence.
ACCESSION   DATIMH010000042 DATIMH010000000
VERSION     DATIMH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586307"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /locus_tag="VLI90_00740"
     CDS             complement(<1..1611)
                     /locus_tag="VLI90_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV12754.1"
                     /translation="MTRLNPRALIVIIAAGLCALTAAIYWPVHRYGYISYDDPYYIYN
                     NPHVQAGFTRDTVHYAFTTSTGGNWNPLLWLSFYADRALLNLRPGAMHVENVALHAVS
                     GVLLLLLLAQLTGAIWRSAAVAALFLCHPMHVESVAWLTERKDVLSTPLLLGTMMTYV
                     AYTRAATPARRLLSYVAMLLLFALSLMVKSMGVTLPALLLLIDFWPLRRRLSARTVLE
                     KIPLFVIVLALLPIAMHAQVAVGATNEVLTLADRFTNAVVTYVLYAVKLIAPTGLAVF
                     YPHPGARPAAQVVSAGLLLLLISAGAFVARHRRPYLFVGWFWFIGTLVPVIGIVQVGS
                     QAMADRYSYLPSIGFFIAVVWFIADAIALFVRSHSPRAWTAATIATVAIASSSILCRQ
                     QLYYWRDSQAIFAHAMEVTGENWVADMHLGDIAFQRGDYATAVEYFQDVIRLRPNDAR
                     GYFDAANCITPSNPRRAIQLYREAIKRDPREPGYHVNLAVALIMIDDRAGALDEAREA
                     LRLDPNYGPAKAAVAELSAPPPPSPAAPP"
     gene            complement(1608..3281)
                     /locus_tag="VLI90_00745"
     CDS             complement(1608..3281)
                     /locus_tag="VLI90_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HSV12755.1"
                     /translation="MDQRIILLPQPRRLVRREGTLAVSGDLLAQARRTTAAPGGHSQG
                     YHLSIAPEGVTIAASDPAGFFYAEQTLRQLLRQFPSALPYVEIDDHPDFPARGVMLDI
                     SRDKVPTISTLFALVEMLAEWKINQLQLYTEHTFAYREHRQVWADASPMTADEIRALA
                     AFCRQRFIELVPNQNSFGHMERWLTRPPYRHLAEAPDGADTPWGFRWHGPFSLCPTDP
                     ASIDFLRGLYAELLPNFSSRLFNVGCDETFDIGQGRSKAECDRRGVTRVYLDFLGKVN
                     ELVQSHGRSMMFWGDIIVKKPELIAELPPGVIAMEWGYEADHPFDRDGESFASAGVPF
                     YVCPGTSSWNSIAGRTDNAIANLRSAAEHGLKHGAIGYLITDWGDNGHLQYLPVSYLG
                     FAAGAAFSWCLESNRDIDLIAALNAHAFVDPTAVLGRVACDLGNVYRVCPKNNVNGSA
                     LFRVLVPPPNDANPKRHLTAADLEAVSAAIDRAIAPLSDAGLIPDEFRNAAAMLRHAI
                     ALAGGASGGVKAAPGAIIGEHRRLWLLRNRPGGLADSAARLAPCPGVLR"
     gene            3541..4158
                     /locus_tag="VLI90_00750"
     CDS             3541..4158
                     /locus_tag="VLI90_00750"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HSV12756.1"
                     /translation="MADAEQVLKNVTLAVLAGGAGSRMGGPKGELIVRGVPILQYLLE
                     RLAWPGPTLLVTAPGREHPPASALFDREVTDAVGDEGPFRGVLTAIEHAGTSIIAIVT
                     VDMPGVTTPQMSWLVQQLPRDSMGVMMKRRIGSDEQVEPFPLVLRASARSLLSELLQR
                     GERSVWRLRQCAGFSVIDAPTDWPEHTWMNLNAPADYNAFVEDEH"
     gene            complement(4224..>5000)
                     /gene="ispH"
                     /locus_tag="VLI90_00755"
     CDS             complement(4224..>5000)
                     /gene="ispH"
                     /locus_tag="VLI90_00755"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HSV12757.1"
                     /translation="FVNHIEEVPAGGVVVYSAHGISPAVRKASKGRQLVEVDATCPLV
                     TKVHMEVLRFVRDGYTILFIGHRDHDEAVGTVGEAPANIIVVESPEEVAQLNVPDPNK
                     LAYVTQTTLSISDAMRTISALKARFPNIKAPPKEDICYATTNRQNAVSDLSPEVDLVL
                     VVGSRNSSNSQRLVETAKLAGRSAYLIDDESELDPNWLQGKKAVLITAGASAPEHLVR
                     ALVERLQRECGGETETRTLIEEDVSFELPRSLRSLAVVSA"
BASE COUNT          909 a         1575 c         1675 g          841 t
ORIGIN      
        1 cggcggggcc gccggcgagg gcggcggcgg cgcgcttagc tctgccaccg ctgccttggc
       61 cgggccgtag ttcggatcca ggcgaagcgc ctcgcgcgct tcgtccagcg cccccgcgcg
      121 atcatcgatc atgatcagcg caacggccag gttcacatgg taccccggct cccgcgggtc
      181 gcgcttgatc gcttcgcgat acaactggat ggcccgccgc gggttgctcg gtgtgatgca
      241 gttggcggcg tcgaaatagc cacgggcatc attgggccga aggcgaatga cgtcctgaaa
      301 atattccacg gccgtcgcat aatcgccgcg ttgaaaagcg atgtcgccca ggtgcatgtc
      361 ggccacccag ttctcgccgg tgacctccat cgcgtgcgcg aagatcgctt ggctgtcgcg
      421 ccagtagtag agctgctggc gacacaggat cgacgacgag gcaatcgcga cggtggcgat
      481 tgttgccgcc gtccatgcgc gcggcgaatg tgatcgaacg aacaaggcga tcgcatcggc
      541 gatgaaccag acgacggcga tgaagaaccc gatcgacggt agatagctat atcgatcggc
      601 catcgcctgc gatccgacct gcacgatgcc gatgaccggc acgagcgtgc cgatgaacca
      661 gaaccagccg acgaacaggt aaggccggcg gtggcgggcg acgaaagcgc cggcggagat
      721 cagcagcagg agcaatcccg cagagaccac ctgcgccgcc ggtcgcgcgc ccgggtgcgg
      781 atagaagacg gccagtcccg tcggcgcaat cagcttcaca gcgtacagca cgtacgtcac
      841 caccgcgttg gtgaatcgat ccgccagtgt aagcacttcg ttggtcgccc ccacggcgac
      901 ctgcgcgtgc atcgcgattg gcaacagcgc gaggacgatc acgaacagtg ggatcttctc
      961 cagcacagtg cgcgcgctca gacgccggcg cagcggccag aagtcgatca gcagcaacag
     1021 cgccggcagc gtgacgccca tcgatttgac catgaggctc aaggcgaaga gcaggagcat
     1081 cgcgacatag ctgaggaggc gccgcgccgg cgttgctgcg cgcgtgtacg cgacgtacgt
     1141 catcatcgtg cccagcagca gcggcgtgct gagcacgtcc ttccgctccg tcagccaggc
     1201 gacggactcg acgtgcatcg ggtgacagag gaacagcgcc gccaccgcag cacttcgcca
     1261 gatcgcgccg gtcaattgcg cgagcagcag cagaagcaac acgccggaca ccgcgtgaag
     1321 agcaacgttc tcgacgtgca tggcgccggg ccggaggttg agcagcgcac ggtcggcgta
     1381 aaacgagagc cagagcagcg ggttccagtt cccaccggtg ctggtggtga aggcgtagtg
     1441 aaccgtgtcg cgcgtgaatc ccgcctgcac gtgcgggttg ttgtagatgt aatacggatc
     1501 gtcgtagctg atatagccgt agcggtggac tggccaatag atggcggccg tgagggcgca
     1561 caaaccagcg gcgatgatga cgatcagcgc gcgtgggttg aggcgcgtca tcgtaacaca
     1621 cccgggcacg gcgcgagccg cgcggcgctg tcggccaatc cacctggccg attgcgaagg
     1681 agccaaagcc ggcggtgttc gccgatgatc gcacccggcg ccgccttaac gccgcctgat
     1741 gcgccgcccg ccaacgcgat cgcgtgccgc agcatcgcgg cggcgttgcg gaattcgtcg
     1801 gggatcagtc ctgcgtcgct caacggcgcg atcgcccgat caatcgcggc cgacacggct
     1861 tcgagatcgg ccgccgtcaa gtgccgcttc gggttcgcat cgttcggcgg cggcacgagc
     1921 acgcgaaaca gcgcgcttcc attcacgttg tttttcggac aaacacgata gacattgccg
     1981 agatcgcagg cgacgcggcc tagcacggcg gttggatcga cgaacgcgtg agcattcagc
     2041 gccgcgatga ggtcgatgtc gcgattggac tcgaggcacc agctgaacgc cgccccggcc
     2101 gcgaatccga ggtagctgac cggcaggtat tgcaggtggc cgttgtcgcc ccagtcggtg
     2161 atgaggtaac cgatcgcgcc gtgtttcagg ccgtgctcgg cagcgctgcg gaggttggcg
     2221 atcgcattgt cagtgcgacc ggcgatgctg ttccagctcg acgtgccggg gcaaacatag
     2281 aacggcacgc cggcgctggc gaacgattcg ccatcgcggt cgaagggatg atccgcctcg
     2341 tatccccact ccatcgcgat cacgcccggc ggcaactcgg cgatcagctc cggtttcttc
     2401 acgatgatgt cgccccagaa catcatcgac cgcccgtgcg attgcaccag ctcgttcact
     2461 ttgcccagga agtcgaggta cacgcgcgtc acgccgcggc ggtcgcactc ggccttgctg
     2521 cgcccctggc cgatgtcgaa cgtctcgtcg cagccgacgt tgaacaaccg gctcgaaaaa
     2581 ttcggcagca gctcggcgta gagcccgcgc agaaaatcga tgctggccgg gtcggtcggg
     2641 cacaagctga acggaccgtg ccagcggaat ccccacggcg tgtcggcgcc gtccggcgct
     2701 tcggccagat gccgatacgg cgggcgcgtc aaccatcgct ccatgtggcc aaacgaattc
     2761 tggttcggca ccaattcgat gaagcgctgc cggcaaaatg cagcgagcgc gcggatctca
     2821 tccgccgtca tcgggctcgc gtccgcccac acctgccgat gctcgcgata cgcgaacgtg
     2881 tgctcggtgt agagctgaag ctggttaatc ttccactcgg ccagcatctc aaccagcgcg
     2941 aaaagcgtgg agatcgtcgg caccttgtcc cggctgatgt cgagcatgac gccgcgagcg
     3001 gggaagtcgg gatggtcgtc gatctcgacg tacggcagcg cagacgggaa ctgccgcagc
     3061 aactggcgaa gggtctgctc ggcgtagaaa aacccggcgg gatcggacgc ggcgatcgtg
     3121 acgccctcgg gtgcgatcga aaggtgatag ccctgcgaat gtccacctgg cgcagcggtg
     3181 gtgcgccgcg cctgcgcgag cagatcaccg gagaccgcga gcgttccctc acgtcggacg
     3241 agccggcgcg gctgcggcaa caggatgatt cgttgatcca acggcggcgt cctcaatcgc
     3301 gtcgaagtat agcgaaggtg cggcgcgatt cacgtacact gatgggcgac gcaggcaaac
     3361 gcacgtctgc agtgcgcgct gctctggtcc cctcgcccgg taccccggga gagggtttgg
     3421 gtgagggtga tttggtctgg cgataaccgc tcgacgttcg aaatcaccct caccccgacc
     3481 tctcccggcg taccgggcga gggggaaaga gcaggatgca attccccgga tgcgcgacct
     3541 atggccgatg cggaacaagt tctgaagaac gtcacgctcg cggtgctggc cggcggtgcg
     3601 gggtcgcgca tgggcggacc caagggggag ctgatcgtcc gcggggtgcc gatccttcag
     3661 tatctgctgg agcggttggc gtggccggga ccgacactgc tggtcaccgc gccggggcga
     3721 gaacatccgc cggcgtcggc attgttcgat cgcgaagtga ccgatgccgt cggggatgaa
     3781 ggcccctttc gcggtgtgtt gacggcgatc gaacacgcgg gcacgtccat catcgccatc
     3841 gtgaccgttg atatgccggg cgtcacgacg ccgcagatgt cgtggctggt tcagcaactg
     3901 ccgcgggatt cgatgggcgt gatgatgaag cggcggatcg gaagcgatga acaggtcgag
     3961 ccgtttccgc tggtgctgcg agcgagcgca cgatcactgc tgagtgagtt gctacagcgt
     4021 ggtgagcgca gcgtgtggcg gctgcgccag tgcgccggat tctctgtcat cgatgcgccg
     4081 accgattggc ccgagcacac gtggatgaac ctgaacgcgc ccgccgacta caacgcgttc
     4141 gtcgaggacg aacactaacc gcaaacaaag aagccccgca tgtccatgcg gggctccgat
     4201 gtatcttgaa gtttacttgc ggatcacgcg ctgacgacgg ccaggctgcg cagcgaacgc
     4261 gggagttcga acgacacgtc ttcctcgatc agcgtgcgcg tttcggtctc gccgccgcac
     4321 tcgcgctgca ggcgttcgac gagcgcgcga acgaggtgtt cgggggcgct ggcgccggcg
     4381 gtgatcagca ccgctttttt gccttgcagc cagttggggt ccagttcgga ttcatcgtcg
     4441 atcaagtacg ccgaccgccc ggcaagcttc gccgtctcga ccagccgctg agaattcgaa
     4501 ctgttccggc tgccgacgac cagcaccaaa tccacttccg gcgacaggtc gctcaccgcg
     4561 ttttggcggt tcgtcgtcgc gtagcagatg tcttccttcg gcggggcctt gatgttgggg
     4621 aagcgcgcct tcagtgccga gattgtccgc atcgcatcgc tgatgctcag cgtcgtctgc
     4681 gtcacgtacg ccagcttgtt cgggtccggc acgttcagct gggcgacctc ttcgggcgat
     4741 tcgaccacga tgatgttggc cggcgcctcg ccgacggtgc cgacggcttc gtcgtggtcg
     4801 cggtggccga tgaacaggat cgtgtaacca tcgcggacga accgcagcac ttccatgtgg
     4861 acctttgtta ccagcgggca ggtggcgtcg acctcaacca gctgccggcc cttggacgcc
     4921 ttgcggaccg ccgggctaat gccgtgggcg ctgtagacca ccaccccgcc ggcgggcacc
     4981 tcttcaatgt ggttgacgaa
//
LOCUS       DATIMH010000043         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3473729, whole genome shotgun sequence.
ACCESSION   DATIMH010000043 DATIMH010000000
VERSION     DATIMH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3473729"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1232
                     /locus_tag="VLI90_00760"
     CDS             261..1232
                     /locus_tag="VLI90_00760"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSV12758.1"
                     /translation="MARMLITGGAGFIGSHLAERLLADGHHVILLDDLSTGRTENVQA
                     LLDRPNIQLVRDSVENVSTVNTLMAQCDAVFHLAAAVGVQLIIDEPVRTIRSIIHGTE
                     VVLEAAMRFGRPVLITSSSEVYGKGARVPFDEDDDVLVGPTKHSRWCYAYAKGVDEFL
                     GLSYHTQFGLPVTIVRLFNTVGPRQVGMYGMVLPRFVAAALAGKPLHVYGDGRQTRCF
                     CHVSDVVAALDQLMSSPQHAGQVFNLGSDQEISISQLAHRVIELCGSHSLIEYISYEK
                     AYGQAFDDLARRVPRLDRIRAAIGFQPKHDLDQIVRSVIDDQKRTAP"
     gene            1469..2818
                     /locus_tag="VLI90_00765"
     CDS             1469..2818
                     /locus_tag="VLI90_00765"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HSV12759.1"
                     /translation="MLSYLAQATTMVAAAAGDAVATSQPVASATADEVLSPYVYVFYA
                     AFLVAFFFTPIMRQVALYYGIIDEPDLVRKLHTSPVAYLGGVAVFLGWLAGLAISQFA
                     SVHRPDSDLLHLQVPVPILIAAAMIVILGLWDDIRKVQPWFKITVQIAAALVLLYGGI
                     GSHAMEPLFAPVQQRIEHYYHWTPAWYETFVMVGSGFITVGLVVACCNATNLMDGLDG
                     LCGGVTAVIAAGFVFLAVHMAMYGRMENANADGMRVVLGLALLGAVLGFVPFNFNPAS
                     IFLGDTGSMFVGFACATLIVMMAEEKPRWFLASMVMFALPVLDTTLAFARRWVNKRPL
                     FSADRHHFHHQLVARGFTVKQTVIISYGLATAFVLLGGAIVFIRTRYVAAVYMVVFGS
                     IIVAAYKMGMVHEKPRVVTRRPLGADALLAAPSEVEPGQVLEVRDNEHGGGNGKPAV"
BASE COUNT          485 a          898 c          936 g          573 t
ORIGIN      
        1 tcgccacctc gtgtggcgac ggcccaatcc gttggccctg cccgcttcgg gctcgcgatc
       61 tctcttcgtt gcaataacta catctggaca acaaccggag taacggcgcg attcccgaac
      121 gcgccgtccg ccgcgcacgc gacacatgtc aatcgttcgg cgtgcgatcc gtcgcccgtc
      181 gccggctgac ctttcgtgcg cgcccgccat tctatccgct ttgcgggcaa cgtgtggagc
      241 gtctatccta aggcctcacc atggcgcgaa tgctcattac cggcggcgcc ggattcatcg
      301 gctcacatct ggccgagcgg ctgctggccg acgggcacca cgtcattctg ctcgatgacc
      361 tctcgaccgg ccggaccgag aacgtccaag ctctcctcga tcgcccaaac atccaactgg
      421 tgcgcgactc ggtcgagaat gtctcgaccg tcaacacgct gatggcccag tgcgacgcgg
      481 tgtttcacct ggcggccgcg gtgggcgtgc agctgatcat cgacgagccg gtgcgcacga
      541 tccgcagcat catccacggc accgaggtgg tgctggaagc ggcgatgcgt tttggccgac
      601 cggtgctcat cacgagttca tccgaagtgt acggcaaggg cgcgcgcgtg ccgttcgatg
      661 aagatgacga cgtgctggtg ggcccgacga agcacagccg atggtgctac gcgtacgcca
      721 agggcgtcga tgagttcctc gggctgtcgt atcacacgca gttcggtttg ccggtgacga
      781 tcgtgcggct tttcaacacg gtcggcccgc ggcaggtggg catgtatggc atggtgctgc
      841 cgcggttcgt cgccgcggcg ctggcgggca agccgctgca cgtctacggc gacggccggc
      901 agacgcggtg cttctgtcat gtgagcgacg tcgtggcggc gctggatcag ttgatgtcgt
      961 cgccacagca cgcggggcaa gtattcaacc tgggcagcga tcaggaaatt tcgatctccc
     1021 agctggccca ccgcgtgatc gaattgtgcg gcagccacag cctcatcgaa tacatctcgt
     1081 acgaaaaagc gtacggccag gcgttcgacg acttggcgcg gcgcgtgccg cgactggatc
     1141 gaattcgcgc agcgatcgga ttccaaccga agcatgactt ggatcagatc gtccgctccg
     1201 tgatcgacga tcagaagcgc accgcgccgt aagcgcgacc ttaatagccc aggccggaag
     1261 gccaagggat cggattgcaa atcaatgcag cttgtcgacc aatccccggc cttccggtat
     1321 gggctatcaa tgcctcatct atcggacttg cctcgctctt gaccgcgatg cgctgcgctg
     1381 gagttgagcg cctatccgga cgatgacgac tttatcgagg tctcgaacgc cctgccggcc
     1441 tcgatcgtaa gatcatggaa gacgcgcgat gctgagctat cttgcacaag caactacgat
     1501 ggtggccgcc gccgcgggtg acgcggtggc gacctcgcaa ccggtcgcca gcgcgacggc
     1561 ggacgaggtg ctctcgccgt acgtctacgt gttttacgcc gcgttcctcg tcgcgttttt
     1621 cttcacgccg atcatgcggc aggtcgcgct ctactacggc atcattgacg agccggactt
     1681 ggtgcggaag cttcacacct cgcccgtcgc gtatctcggc ggggtcgcgg tgttcctcgg
     1741 ttggctcgcg gggttggcca tcagccagtt cgcaagcgta catcggccgg attcggacct
     1801 gctgcacctg caagtccccg tgccgatcct gatcgcggcg gcgatgatcg tcatcctggg
     1861 attgtgggac gacatccgca aagtgcagcc gtggttcaag atcaccgtgc agatcgcggc
     1921 cgcgctggtg ctgctgtatg gcggcatcgg gtcgcacgcg atggagccgc tgttcgcgcc
     1981 ggtccaacag cgcatcgagc attactacca ctggacgcct gcgtggtacg aaacattcgt
     2041 gatggtcggc agcgggttca tcaccgtcgg cctggtcgtc gcttgttgta acgcgaccaa
     2101 tttgatggac ggcctcgacg gcctgtgcgg cggggtgacc gcggtcatcg ccgcggggtt
     2161 tgtcttcctt gcggtgcaca tggcgatgta cggccgaatg gaaaacgcca acgccgacgg
     2221 catgcgcgtc gtgctcgggt tggcgctgct cggcgccgtt ctcggtttcg tccccttcaa
     2281 cttcaacccc gcgtcgatct tcctcggcga caccggcagc atgttcgtcg ggttcgcctg
     2341 cgcgacgctg atcgtcatga tggccgagga aaagccgcgg tggttcctgg cctcgatggt
     2401 gatgttcgcg ctgccggtgc tggatacgac gttggccttc gcccgtcgct gggtgaacaa
     2461 gcggccgctg ttcagcgcgg accggcatca cttccaccac cagcttgtcg cgcgcgggtt
     2521 cacggtcaaa cagacggtca tcatcagcta cggcttggcc accgcgttcg tgctgctggg
     2581 cggcgcgatc gtcttcatac gcacgcgcta cgtggccgcg gtgtacatgg tggtgttcgg
     2641 ctcgatcatc gtggcggcgt acaagatggg gatggtgcac gaaaagccac gagtggtgac
     2701 gcgacgccca ttgggggctg acgcgctgct ggcggcgcca agcgaagtcg aaccggggca
     2761 agtcttagaa gtgcgcgaca acgaacacgg cggcgggaat ggaaaaccgg cggtgtagtc
     2821 gctcttgccc cctcgcccgg tacgccggga gagggctggg gtgagggtga tttcaattga
     2881 caattgaaat tt
//
LOCUS       DATIMH010000044         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4340622, whole genome shotgun sequence.
ACCESSION   DATIMH010000044 DATIMH010000000
VERSION     DATIMH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4340622"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1763
                     /locus_tag="VLI90_00770"
     CDS             <1..1763
                     /locus_tag="VLI90_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12760.1"
                     /translation="LGQSIFTYANVPYRIKPYEQIVRNPHDSIVFDHALDREIAARVA
                     CIGADGKLLLDSGDGQPYHVNLAEKLLVPLLAKVCNLVIDGGIWMNTQRPEWNDANNA
                     LAGNGLSVVTLCYLRRYVKFLLELLEPLAGQSVDISSAVTDWLTSTRQALSKTGVRRR
                     QLLDELGSAFCAYRQRVYTAGVSGKTNVAVDAIADLLRRVFDRVDRSIRANRRGDGLY
                     HSYNLLKLKDDEASVGHLPEMLEGQVAVLSSGCVNAAESLAILHALFKSRMYRPDQDS
                     FTLYPDRAVPSFMDRNVIPDEQVAAIPLLQSMLEVGDTSIVARDADGVCRFNGDFLNA
                     AGVRAAMDRLSYHSAADAVAQLFQEIFDHHRFIGRSGVMHGYEGLGCIYWHMVAKLLL
                     ATQEQFWAAQREHQPWETTLQPLAQMYYQIRQGLGFGKSAKQFGAFPTDPYSHTPPHS
                     GARQPGMTGQVKEEILARFGELGLIVDDGCIRFHPILLRRREFLTQPTTWNFIDVNGD
                     GRQLSLPAGSLGFSYCQVPIIYHLADEPMRIAATSATGVRTTTPADSLDRSTSAALFA
                     RDATIARIDVTVPVELLRLA"
     gene            complement(2023..>2293)
                     /locus_tag="VLI90_00775"
     CDS             complement(2023..>2293)
                     /locus_tag="VLI90_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12761.1"
                     /translation="KDGLPGLMLNPFGQWWLTTRELAANSRAPLLMRHLRGARWTYVY
                     LYISMGRCVAEIKQQKDLMSEQAAKSAKNATPIPRSCILAVFCGH"
BASE COUNT          434 a          721 c          691 g          447 t
ORIGIN      
        1 tgctcggtca atcgatcttc acctacgcca acgtgccgta tcgcatcaag ccgtacgagc
       61 agatcgtgcg caatccgcac gactcgatcg tgttcgacca cgcgctggat cgcgagatcg
      121 ccgcccgggt ggcgtgcatc ggcgcggacg gcaagctgct gctcgacagc ggcgacggtc
      181 aaccttatca cgtcaacctg gctgaaaaac tgctcgttcc gctgctggcc aaggtgtgca
      241 acctcgtcat cgacggcggc atctggatga acacccagcg gccggaatgg aacgacgcga
      301 acaacgcgct ggccggtaat gggctgtcgg tcgtcacgct ttgctatctg cggcgatacg
      361 tgaagttcct gctggaactg cttgagccac tggcggggca gtcagtggac atctcgtcgg
      421 cggtaaccga ctggctcaca tcgacgcggc aggcgctttc gaaaacgggc gttagaagac
      481 ggcaactgct ggacgaacta ggcagcgcgt tttgcgcgta ccgccagcgc gtctacaccg
      541 cgggtgtttc gggcaagacg aacgtcgcag tcgatgcgat tgcggatttg ctgcggcgcg
      601 tgttcgatcg cgttgatcgt tcgatccgcg ccaatcgccg cggcgatggc ctgtatcact
      661 cgtacaacct gttgaagctt aaggacgacg aggcgtcggt cggccacctg ccggaaatgc
      721 tcgagggaca ggttgcggtg ttaagcagcg gctgcgtcaa cgcggcggaa tcgttggcga
      781 ttctgcatgc gctgttcaag agccggatgt atcgcccgga ccaggacagc ttcacgctgt
      841 atcccgatcg ggcggtgccg tcgttcatgg atcgcaatgt gatccccgac gagcaagtcg
      901 cggcgatccc gctgctgcag tcgatgctgg aagtcggcga cacgtcgatc gtcgcgcgcg
      961 acgccgacgg cgtctgccgg ttcaacggcg attttctaaa cgccgccggg gtgcgggcgg
     1021 cgatggaccg actcagttac cactccgccg ccgacgcggt cgcgcagctc tttcaggaaa
     1081 tattcgacca ccatcgcttc atcggccgct ccggcgtgat gcacggctac gaaggcctcg
     1141 gctgcatcta ttggcacatg gtcgccaagc tgctgctggc aacgcaggaa cagttctggg
     1201 ccgcccagcg cgaacatcag ccgtgggaga caacgctgca gccgctggcg cagatgtatt
     1261 accagattcg ccagggcctg ggcttcggca aaagcgcgaa gcagttcggc gcatttccga
     1321 ccgatcccta ttcgcacacg ccgccgcaca gcggggcgcg ccagcccggc atgaccgggc
     1381 aggtgaagga agaaatcctc gcgcgcttcg gcgaactcgg attgatcgtc gatgatggct
     1441 gcatccgctt ccaccccatc cttctccgcc gccgcgagtt cctgacgcag ccgacgacgt
     1501 ggaacttcat cgacgtgaac ggcgacggcc ggcaactgtc gctgcccgcc ggctcgctcg
     1561 gcttctccta ctgccaggtg cccatcatct accacctcgc cgacgagccc atgcgaatcg
     1621 cggcgaccag cgcgaccggc gtccgcacca caacaccggc agattcgctc gatcgttcca
     1681 cctccgccgc gctattcgcg cgcgatgcga cgatcgcccg gatcgacgtc accgtaccgg
     1741 tggagttgtt gcgactggcg taacgcgtta cccgggaagc gctgaccctt catcaatcca
     1801 aggtcccacg taccgcggcg tcgcgccaaa cgaatgcccg cggcatcacg atctggcaat
     1861 cagtgatgtc cccttatccg ttcccttatc cgttctctgg caatcagtga tgtcccctta
     1921 tccgttctcg ttctttcgcc aagtgggagc tgattcctag acttgagatg gtcaaggcga
     1981 gcaaaaaggg gcacgcgaaa ccggaacgct tttggtcgca ggtcaatggc cacaaaaaac
     2041 ggcgaggatg catgagcgcg ggattggcgt ggcgttcttc gcgcttttgg cggcttgttc
     2101 actcattaag tccttttgct gcttgatttc ggcgacgcat ctgcccatag atatgtatag
     2161 atacacatac gtccatcgcg cgcctcgtaa gtgtcgcatc aaaagtggcg cgcgggagtt
     2221 cgcagcaagc tcgcgggtgg ttaaccacca ttgtccaaag ggattaagca tcaaacctgg
     2281 caagccgtcc ttt
//
LOCUS       DATIMH010000045         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2608255, whole genome shotgun sequence.
ACCESSION   DATIMH010000045 DATIMH010000000
VERSION     DATIMH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2608255"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..762
                     /locus_tag="VLI90_00780"
     CDS             94..762
                     /locus_tag="VLI90_00780"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HSV12762.1"
                     /translation="MLHLTRDQVRRIDRLAGERYHIPGIVLMENAARAVADEACRMLG
                     GECVGEILILCGGGNNGGDGLTVARHMHNRGAEVAIGLLTDPSRYQGDALINWQIAQA
                     MGLQASPFAPAMLEDPRPMLIIDAIFGTGLTQSPRDPFPHIASIINHAGIPVLAVDVP
                     SGLDCDTGEPPGACISATRTVTFVARKVGFKNAAAHRVLGDVIVGDIGCPRELIEEIA
                     QAQS"
     gene            complement(881..1294)
                     /locus_tag="VLI90_00785"
     CDS             complement(881..1294)
                     /locus_tag="VLI90_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HSV12763.1"
                     /translation="MLPTSAKAEELRRQPPKPEAAAPETPPPLDPIKSIEHKLLEGKI
                     IEALRQVYDPEIPVSIYDLGLIYGIDIADDKSVKIRMTLTAPACPVAGSLPGEVERKV
                     QQIPEVKDVEVELVWEPQWDKSMMSEAAQLQLGFL"
     gene            complement(1414..>2869)
                     /locus_tag="VLI90_00790"
     CDS             complement(1414..>2869)
                     /locus_tag="VLI90_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSV12764.1"
                     /translation="EIIYTRGTTESLNLVASSLGRLRLRENDEVVVSAMEHHSNIVPW
                     QMICGQTGAKLRVIPMNERGELLLDEYDKILAGGRVKIVAVVHLSNSLGTVNEIKRIA
                     EMAHGVGALMFADGAQWVAHYPTDLQDLGVDFYAFSGHKLFGPTGVGVLYGRRELLEK
                     MPPYQGGGDMIESVTFEKTTYAPLPNKFEAGTPDIAGVIGLGAAIEYVQSIDLNAVHV
                     HEQALLDHATEQLRQIPGLRIIGTARNKGSVVSFVLENPPIASIDIGTALDQRGVAVR
                     TGHHCCMPVMARMNIAATTRASFAMYNTRGDVDALVAGLREIVAKAAKKPAAAAKSAK
                     QEVEYPKAVADSPTTAAEELAGDFDLLGEREARSQYVIDLGERLPNYFDLLKKVTPRV
                     PGCMSEVYLVARKAPGSSDTLEFVADADAAIVRGLIALLERLFSGQRAADILAFDVES
                     FFRRIGLDQFVSSQRRNGLAGMIARIRREAEGLK"
BASE COUNT          500 a          946 c          873 g          550 t
ORIGIN      
        1 gatgctgcgc gggaaattaa ttcttcgcat acttcttggc tagaatcctt cgactccggc
       61 ggagtaccgt ctgcgctcag gatgaattac gaaatgctcc acctcacgcg cgaccaggtt
      121 cgccgcatcg atcgccttgc gggcgagcgt taccacatcc ccggcatcgt gctcatggag
      181 aacgccgccc gcgcggtcgc cgacgaggcc tgccgcatgc tcggcggcga gtgcgtcggt
      241 gaaatcctca tcctctgcgg cggcgggaac aacggtgggg atggcttgac cgtcgcccgc
      301 cacatgcaca atcgcggcgc cgaagtggcg atcggtcttc tgaccgatcc gtcgcgatat
      361 cagggcgacg cgctgatcaa ctggcagatc gcgcaggcga tgggacttca ggcatcaccc
      421 ttcgcgcccg cgatgctcga agacccgcgg ccgatgttga tcatcgatgc gatcttcggc
      481 accggcctga cccaatcacc gcgcgatccg ttcccgcaca tcgcgtcgat catcaatcac
      541 gccggcatcc ccgtgctggc ggtggacgtg cccagcggcc tcgattgcga caccggcgag
      601 ccaccgggcg cctgcatcag cgcgacaaga acggtgacgt tcgtcgcgcg caaagtcggc
      661 ttcaagaacg cagcagccca tcgcgtgctc ggcgacgtga tcgtcggcga catcggctgc
      721 ccgcgggagt tgatcgagga gatcgcgcag gcccagtcgt agccgcaggc gacagccagc
      781 ggggcgctgg gatgtcaacc cggttggtgc tcgcgaagac cccgctggct gtcgcccgcg
      841 gctatgaaat cggcgcgagc gacaacctcg tgcgatgcag ctacaaaaat cccaactgca
      901 gctgggccgc ctcgctcatc atcgatttgt cccactgcgg ttcccacacc agctcgacct
      961 cgacgtcctt cacctcgggg atctgctgca ccttgcgctc gacctcgccc ggcagtgaac
     1021 cagccaccgg acatgccggg gcggtcagcg tcatgcggat cttcaccgac ttgtcatcgg
     1081 cgatgtcgat gccgtagatc aggccgaggt cgtagatgct gacggggatc tcggggtcgt
     1141 acacctggcg gagcgcttcg atgatcttgc cttcgagcag cttgtgctcg atggatttga
     1201 tgggatcaag cggcggagga gtttcgggcg ccgccgcttc tggctttggc ggctgccgcc
     1261 gcagttcttc agccttggcg gaagttggca acaccggcaa gctcgcgacc gaatcaggat
     1321 gtttatattc gtctgacata agtagcacgg gctccgagcc tgtggctctc tcgcccgtgt
     1381 cagagcacct tggcaccggc tctggcgccc gtgctacttt aacccttccg cttctcttcg
     1441 aattcgcgcg atcattcccg ctagaccgtt gcggcgttgc gagctgacga actggtccag
     1501 tccgattcga cggaagaacg actccacatc gaacgcaaga atgtccgcgg cgcgctgacc
     1561 ggaaaaaagg cgttcgagca gcgcgatcaa cccgcgcacg atcgcggcgt cggcgtcggc
     1621 gacgaactcc agcgtgtcgc tcgaccccgg cgcttttcgc gccaccaagt acacctcgct
     1681 catgcacccc ggcacacgcg gcgttacctt cttcagtaga tcaaaataat tgggcaaccg
     1741 ctcgcccagg tcgatcacgt actggcttcg tgcttcgcgc tcgccgagca gatcaaaatc
     1801 gcccgccagc tcttccgcgg ccgtcgttgg cgagtccgcc accgccttgg gatactccac
     1861 ttcctgcttc gcggatttcg ccgccgccgc gggtttcttc gcggctttgg cgacgatctc
     1921 acggaggccc gccaccagcg catccacatc gccgcgcgtg ttgtacatcg cgaacgacgc
     1981 ccgcgtcgtc gcggcgatgt tcatgcgggc catcaccggc atgcagcagt gatgcccggt
     2041 gcgcacggcg acgccgcgct gatcgagcgc ggtgccgatg tcgatcgatg cgatcggcgg
     2101 gttctccagc acgaaggaga ccacgctgcc cttatttctg gcggtgccga tgatgcgcag
     2161 gccggggatt tgccggagtt gttccgtcgc gtgatcgagc agcgcttgct cgtgcacgtg
     2221 caccgcattc agatcaatcg actgcacgta ctcgatcgcc gccccgagcc cgatcacgcc
     2281 cgcaatgtcc ggcgtgccgg cctcgaattt attcggcagc ggcgcataag ttgtcttctc
     2341 gaacgtcacg gactcgatca tgtccccacc gccctggtac ggcggcattt tttccagcag
     2401 ctcgcgccgg ccgtacagga caccgacgcc agtagggccg aacagcttat gaccggagaa
     2461 ggcgtagaaa tcgacgccga gatcctgcag gtccgtcgga taatgcgcga cccactgcgc
     2521 gccgtcggca aacatcaacg cgccgacgcc gtgggccatc tccgcaattc gcttgatctc
     2581 gttgaccgtg ccgagcgaat tcgacaggtg cacgacggcg acgattttga cgcgtccgcc
     2641 cgcgaggatt ttgtcgtatt cgtccagcag cagttcgccg cgctcgttca tcgggatgac
     2701 gcggagcttg gcgccggttt gtccgcagat catctgccag gggacgatgt tggagtggtg
     2761 ctccatcgcg gagacgacga cttcgtcatt ttcgcgaaga cgcaagcgac ctagggagct
     2821 tgcaacgaga ttgagacttt cggtcgtccc gcgcgtgtag atgatctcg
//
LOCUS       DATIMH010000046         5435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4340954, whole genome shotgun sequence.
ACCESSION   DATIMH010000046 DATIMH010000000
VERSION     DATIMH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4340954"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VLI90_00795"
     CDS             complement(<1..468)
                     /locus_tag="VLI90_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12765.1"
                     /translation="MLVFLALSLLSLVARAEDIDGVQCAALDQPRVNMVLRRAAHGPM
                     LAGKTLGEEAFNVEAFLDTGASGVLISTNSAQTLGVKREQALNAPEHEARFEDVGVGG
                     GSQFAVSEPLFVSLAPSTADVENKAAVDGIYSLTCGPFRAQVGPLDSGMDMLTA"
     gene            complement(619..2814)
                     /locus_tag="VLI90_00800"
     CDS             complement(619..2814)
                     /locus_tag="VLI90_00800"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSV12766.1"
                     /translation="MAEATTPGAKNVTPTTMPAETSVVGKPIDRVDGRLKVTGGAKYA
                     ADNPVQNLAHAVAVISTIARGKITNLDTSAAEKSPGVLAVLTHQNIGELKTVTREMRP
                     GGQLGQAKPPMTDDLIRYGGQYIALVVAETIEQAKHASRLVKVIYAAEKPSTDIDNPL
                     ELKEKGKTMRGDADGALASAAVKVSQVYSTPVEHHNPMEMHATVAAWDGDRLTLWDST
                     QHVLGVRGVVAAVLGISPENIRVIDPFMGGGFGCKGSVWPNSYLAAVAARHVNRPVKM
                     VVTRQQMFSQTGYRPWTKQQVSLGVDSDGKLVSLKHESTCTTASFEDWVENSGRVSGM
                     LYKCDNVLITQKVARINAGVPVQMRAPGEASGMFALESAMDELAYAAKLDPIELRLRS
                     YADKDYGEKEPLTFTSKSLRECYRVGADKFGWSKRAAQPGQMRDGDELVGYGMATATY
                     PAGQNGASASIRLQSDGNVIVTTAGHDLGTGTYTICAQIAAETLGVSADKVRVQLGDT
                     TLPRAPGAGGSTQAVTVGAAVQAAAQGLLRQCATLLDRDAADMAGGGEKLMAVFEKNG
                     GRPVSFTANSVPDPSIRGKYARYSFGAQFAEVRVNPRLGKARVTRWVGAFAAGRILNP
                     KTARSQLIGGIIMGMGMALLERTVYDPVRGKVVNDNLADYQVPVNADVPEIEIVFVEE
                     RDEHVNSLGAKGVGELGITGAPAAIANAVYHATGVRVRDLPISVDRLLT"
     gene            complement(3079..5391)
                     /locus_tag="VLI90_00805"
     CDS             complement(3079..5391)
                     /locus_tag="VLI90_00805"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSV12767.1"
                     /translation="MPDSTLDKPAATPIGRPIDRVDGRLKVTGQARYAAEAPVQGVAH
                     AVLVESNIARGTIASIDTSAAEAQPGVLAVVTSKNMPKLSDPRKGGGMTGENRLPLSD
                     DNILYTGQHVAVVVANTLEQARHAANLVKVAYDPEPPMVALHDVRAEVVRPEKNFGEP
                     LQVTIGDVDAALKANPAVVVQQTYTTPVEHHNPMEMSATVAAWDGDDRLTVWDSTQYM
                     MGTRATLAAAFGLPAENVRTICPFVGGAFGCKGAQWPHTLLAAAAAKVVRRPVKLMLT
                     RQQMFTSCGHRPPTEQTLTLAADSSGKLLALRHETNQQASIANDFIEPCGIGTSRILY
                     ATPAMHITHAARKMNVGSPTFMRAPGETPGTYALECAMDELAVALKMDPVALRRANHA
                     DVQPENKKPWSSKHLRECYQMGIEKFGWSKRKPEPRSMRHDDGRLIGWGMATATYPGY
                     QFPGTARIRLMSNGSDGVRAVGSSATHDLGTGAYTVCTQITAGLVGLPLDRVKFELGD
                     STLPPAPVSGGSTTTASVGQALTVAADALKSALLKLATDGNPSSKLAGAKLSDLVMRD
                     GKLMLASDPSRSEDLAALVARAGRAYVEGTSPPPGEPPQARHAASDDAEGEDYAANQK
                     KYAFQSFGAHFVEVMIDEPIGRCRVTRVVSVMDVGRVINPKTAGSQVMGGVIMGIGMA
                     LMEETAYDQRSGKPVTNNLADYPVCVNADIHSIEPYFIDIPDPRMNAVGCRGVGEIGI
                     TGVAAAVANAVYHATGKRIRDLPITTDKLM"
BASE COUNT          869 a         1866 c         1662 g         1038 t
ORIGIN      
        1 cgcggtcagc atgtccatcc cgctgtccag cgggccaacc tgcgcacgga aaggtccaca
       61 cgtcaggcta tagatgccgt ccaccgcggc cttgttctcg acgtccgccg tcgatggcgc
      121 gagcgacacg aacagcggct cggacaccgc gaactggctg ccgccgccca cgccgacgtc
      181 ttcgaaccgc gcttcgtgct cgggcgcgtt cagcgcctgc tcgcgcttca ccccgagcgt
      241 ctgggcgctg ttggtggaga tcagcacgcc gctggcgccg gtgtcgagga acgcttcgac
      301 gttgaaggct tcttcgccga gcgtcttgcc ggccaacatc ggcccatggg cggcgcggcg
      361 caggaccata ttgacgcgcg gctgatcaag ggcggcgcat tgcaccccgt cgatgtcttc
      421 ggcgcgcgcg acgagggaaa gcagtgatag cgcgaggaat acgagcaacg ctcgtgtcca
      481 gccgcttgcg gcttcgcaga cccagcggca ccggtttgcc aaaccgcagg cgacgtcatg
      541 ttcgtttccg cagctcacgc ttcaccgact cctggctaga tccttcgact ccggcggagc
      601 gccgcctgcg cttcaggatt aagttaaaag ccgatccacc gaaatcggca gatcgcgcac
      661 gcgcacgccc gtcgcatgat ataccgcgtt cgcgattgcc gccggggcgc cggtgatgcc
      721 caattcaccc acgcccttgg cgccgagcga gttcacgtgc tcgtcgcgct cctccacgaa
      781 gacgatctcg atctccggca cgtcggcgtt taccggcacc tggtaatccg ccaggttgtc
      841 gttgacgacc ttcccgcgca ccgggtcata taccgtccgc tccagcaacg ccatgcccat
      901 ccccatgatg attccgccga tcaactggct ccgcgccgtt ttcggattga ggattcgccc
      961 cgcggcgaac gcgccaaccc atcgcgtcac gcgcgccttg cccagccgcg ggttcacgcg
     1021 cacctcggcg aactgcgcgc cgaacgaata gcgcgcgtac ttgccgcgga tggacggatc
     1081 gggcaccgag ttggcggtga atgacacggg tcggccacca ttcttctcga acaccgccat
     1141 caacttctca ccgccgccgg ccatgtcggc tgcatcacga tcgagcagcg ttgcgcactg
     1201 ccgcagcagt ccttgcgctg ccgcctgaac cgccgcgccg acggtgaccg cttgcgttga
     1261 accgcccgcg ccgggcgcgc ggggcagcgt cgtatctccc agttgcacgc gcaccttgtc
     1321 cgccgaaacg ccgagcgttt ccgccgcgat ctgcgcacag atcgtgtacg tgcccgttcc
     1381 caggtcatgc cccgcagtgg tgacgatcac gttgccatcg gactgcagac ggatcgacgc
     1441 cgacgccccg ttctgccccg cgggatacgt cgccgtcgcc atgccgtagc cgacgagctc
     1501 atcgccgtcg cgcatctgtc ccggctgggc ggcgcgcttc gaccagccga atttatcggc
     1561 gccgacgcgg taacactcgc gcagcgactt gctcgtgaac gtcagcggtt ccttctcgcc
     1621 atagtccttg tcggcataac tgcgcaggcg aagctcaatg gggtcgagct tcgccgcgta
     1681 cgccagctcg tccatcgccg actccagcgc gaacatcccg cttgcttcgc cgggcgcgcg
     1741 catctgcacc ggcacacccg cattgatgcg ggcgaccttt tgcgtgatca gcacgttgtc
     1801 gcacttgtac aacatgccgc tcacacggcc ggagttttcg acccagtctt cgaacgaggc
     1861 tgtggtgcaa gtgctctcgt gcttcagcga cacgagtttt ccatcactgt ccacgcccag
     1921 cgacacctgc tgtttcgtcc acgggcgata gccggtctgg ctgaacatct gctgccgcgt
     1981 gacgaccatc ttcaccgggc gattcacgtg ccgcgccgcc accgcggcga gatagctgtt
     2041 gggccagacc gatcctttgc acccgaaccc gccgcccatg aacggatcga ttacacgaat
     2101 gttttcggga gaaatgccga gcaccgcggc gaccacgccg cgcacgccca gcacgtgctg
     2161 cgtggagtcc cacagcgtca ggcgatcgcc gtcccacgcg gccaccgtcg cgtgcatctc
     2221 catcgggttg tgatgctcga ccggcgtcga atagacctgc gaaaccttca ccgccgccga
     2281 cgccagcgcg ccgtcggcgt ccccgcgcat cgtcttgccc ttctccttca gctccagcgg
     2341 gttgtcgatg tcggtgctcg gcttttccgc cgcgtagatc accttcacca gccgcgacgc
     2401 gtgcttggct tgctcgatcg tctcggcgac gaccagcgcg atgtactgcc cgccataacg
     2461 aatcaggtca tcggtcatcg gcggcttggc ctgacccagc tgcccgccgg gccgcatctc
     2521 gcgcgtgacg gttttcagct cgccaatatt ctgatgcgtc aacaccgcca gcacgcccgg
     2581 cgatttctcg gcggcggagg tatcgaggtt ggtgatcttg ccgcgcgcga tcgtgctaat
     2641 gaccgcgacg gcgtgggcca ggttctgcac cggattgtcc gccgcatatt tcgcgccgcc
     2701 ggtgaccttc agccggccat ccacgcgatc gattggtttg ccgacaacgc tggtttccgc
     2761 cggcattgtg gtcggggtga cgttcttcgc gcctggggtt gtcgcttcgg ccatgcttgc
     2821 tccaggaata caaattcagc attggaccac gaagacacga agggcacgaa gagaagaggc
     2881 attgacgcgg atgaaatttg gcagctcgga gttaccggaa ttcgcaagtc gatcgcacgg
     2941 gccggaggcc agtgcaatgt gccatcgagc tccaggcgca cgttcattcg catctcctct
     3001 tatccgtgtc catcggtgtt catccgtggg aaaaacgctt tccgtccctt cgtgccttca
     3061 tggttcaaat ccaaccgctc acatcaactt atccgtcgtg atcggcaaat ctcgaatgcg
     3121 ctttcccgtc gcgtgataca ccgcgttggc aaccgccgcg gcgactccgg tgatgccgat
     3181 ctcgcccacg ccgcggcagc ccacagcgtt catgcgcgga tctggaatat cgatgaagta
     3241 cggctcgatc gaatgaatgt ccgcgttcac gcacaccgga taatccgcca ggttgttcgt
     3301 caccggcttg ccgctgcgct ggtcgtacgc cgtctcttcc atcagcgcca tgccgatgcc
     3361 catgatgacg ccgcccatga cctggctgcc ggcggtcttg gggttgatca cccggccgac
     3421 gtccatcacg ctgacgacgc gcgtcacgcg gcagcggccg atcggttcgt cgatcatcac
     3481 ctcgacgaag tgcgccccga acgactggaa cgcgtacttc ttctggttgg ccgcgtaatc
     3541 ttcgccttcg gcgtcatcgc tggcggcgtg gcgtgcctgc ggcggttcgc cgggcggggg
     3601 gcttgtgccc tcgacgtatg cgcgcccggc gcgtgcgacc agcgctgcca ggtcttcgct
     3661 tcgcgacgga tcgctggcga gcatcaactt cccatcgcgc atcacaagat ccgaaagctt
     3721 tgcccccgcg agcttcgacg atggattgcc gtccgtcgcc agtttcagca gcgccgactt
     3781 gagcgcatca gcggcgaccg tcagcgcctg tccgacgctg gccgtggtgg ttgaaccgcc
     3841 tgacactgga gccggcggca gcgtggaatc gccgagctcg aacttcacgc gatccagcgg
     3901 caagccaacc aacccggcgg tgatttgggt gcacacggtg tacgcgccgg tgcccaggtc
     3961 gtgcgtggcg ctggaaccaa cggcgcgaac gccatcgctg ccattggaca ttagccggat
     4021 gcgggcggtg ccggggaact ggtagccggg atacgtcgcg gtggccatgc cccagccgat
     4081 caaccgcccg tcatcgtgcc gcatgctgcg cggctcgggc ttgcgcttcg accagccgaa
     4141 tttctcgatg cccatctggt agcactcgcg gaggtgcttg ctggaccacg gttttttatt
     4201 ctccggctgg acgtcggcgt ggttcgcccg tcgcagcgcc accgggtcca tcttcagcgc
     4261 caccgccaac tcgtccatgg cacattccag tgcatatgtg ccgggcgttt caccgggggc
     4321 gcgcatgaag gtcggagacc cgacgttcat cttgcgggcc gcatgcgtga tgtgcatggc
     4381 cggcgtggcg tagaggattc ggctggtgcc gatgccgcag ggctcgataa agtcgttggc
     4441 aatggatgcc tgctgattgg tctcgtgccg cagcgccagc agcttgccgc ttgaatcggc
     4501 ggcaagcgtg agcgtctgct ccgtcggtgg acgatgaccg caactggtga acatctgctg
     4561 ccgcgtgagc atcagcttga ccggacggcg cacgaccttg gctgcggccg cggcgagcag
     4621 cgtatgcggc cactgcgcgc ccttgcaacc gaacgcgcca ccgacgaacg ggcagatcgt
     4681 ccgcacgttt tccgccggca acccgaacgc cgccgccagc gtcgcgcgcg tgcccatcat
     4741 gtattgcgtc gaatcccaaa ccgtgagccg gtcgtcgccg tcccacgcgg caacggtcgc
     4801 gctcatctcc attggattgt gatgctcgac cggcgtcgta taggtctgct gaacgacgac
     4861 ggccgggttg gctttgagtg cggcgtcgac atcgccaatg gtcacctgca atggctcgcc
     4921 aaagttcttt tcagggcgaa ccacctcggc ccgcacatcg tgcagcgcaa ccatcggcgg
     4981 ctctgggtcg tacgcgactt tcaccaaatt cgcggcgtga cgggcctgct ccagcgtgtt
     5041 cgccacgacg accgccacgt gctggccggt gtaaagaata ttgtcgtcgc tgagcggcag
     5101 gcgattctcg ccggtcatcc cgccgccttt tctgggatcg ctcagcttcg gcatgttctt
     5161 cgacgtgaca accgccagca cgccgggctg cgcttcggcc gcgctggtgt cgatcgaggc
     5221 gatcgtcccg cgggcgatgt tgctctccac gagaacagcg tgcgccacgc cctgcaccgg
     5281 cgcctcggcg gcgtagcgcg cctggccggt gacttttaat cgtccatcga cacgatcaat
     5341 cgggcgaccg atcggcgtgg cagcgggttt atctagtgtg gagtcgggca tagatgctcc
     5401 aggaactgcc acgcgtttgc gcgcgtcgat catgc
//
LOCUS       DATIMH010000047         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4918961, whole genome shotgun sequence.
ACCESSION   DATIMH010000047 DATIMH010000000
VERSION     DATIMH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4918961"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1336
                     /locus_tag="VLI90_00810"
     CDS             341..1336
                     /locus_tag="VLI90_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12768.1"
                     /translation="MKILKWIVIVVVLVIVVGLALVYFNIDRIVKHTVETQGTDQLKV
                     PTTLDGVSLGLFKGTVALNDLNVGSPPGFTAPHMLTLGGLNVDTGGISNLRKEPIHVS
                     SIAVDQPKLVVEFAGTEVNFKKLMDNLPSNPDQNPPPSEKKPTKLIIDDLTVNNAHVV
                     LRGLDTVAGKIPGLSKVANLPAELDLPIPSIDVKNIGNADGAQNGAAIKDVVTTIITQ
                     MAAKAADSDKVPPEVKAVLTGNLDAVKDKLTGMATAELNKVTDQLKAKLPAGIPTSLP
                     ANLPGNLGGLINKATGANGNAPADQNGGNNPPSPADNLLNQGLGALRGATSKPAK"
     gene            complement(1418..>3044)
                     /gene="shc"
                     /locus_tag="VLI90_00815"
     CDS             complement(1418..>3044)
                     /gene="shc"
                     /locus_tag="VLI90_00815"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HSV12769.1"
                     /translation="PHMTKARELIRSLGGAEKCNTFTRFYFACLGQISFDSCPSIPPE
                     VVFLPKWFYFNLYNVSAWTRTMILPLGIVTTLRRVRDVPAELGIAELYVDHAAGNMLG
                     IPKPGFPRTWRDFFLRVDRLLKRYEEAPFPGLRERAMQAAEKWLLDHMADSEGLGAIF
                     PPMVYMLIVFKAMGYPDDHPVVLKAHKDLKDFYIREEAPHPGPLPCVPGRGGTIRLQP
                     CVSPVWDTGIAMHALAETGLSIDSDAAMRGTQWLLDKECKYASDWQRNARPVEPSGWF
                     FEFHNPHYPDVDDTAMAVMSLKRLGGKKAEPAVRRGLNWLLAFQNSDGGWAAFDKTKS
                     RPILEYIPFADHNAMQDPSCPDIAGRVLESLGHNGFRKGQTIVDRAIHFIRREQDKDG
                     PWWGRWGVNFVYGTWQVLAGLRAVGEDMNAEYIQRAIAWLRSVQKPDGSFGESCASYD
                     DPSLKGQGESTASQTAWGTMALLAAAGPHDPAVKSGIDWLITHQAADGAWDEQWFTGT
                     GFPRVFYLKYHLYRHYFPLMALARYRRYLDMSA"
BASE COUNT          638 a          972 c          911 g          523 t
ORIGIN      
        1 cgaacgtgca accttccagg tggtacaccg tctcacgcaa aataagcgtg caggcgatga
       61 agttgcagcg gacgaacgcc tgaccgctga tgtagacggt ctggttcaaa aatgtttcgt
      121 tctcgtgctt ggtcaacagc atgggttggg gacctcccgg gcccgcgtca gactcaaaga
      181 tgttccgccg attcgccccc gccaatcggg gaagcggggt cggaaatagt ggcacacccc
      241 ttatcgggtg tcaaatcagg tgacttggga atttccattg tcatttggtc gatacagggg
      301 aacaatcgcc ttctccaaag gcaaacaatc gaggaacgcc atgaaaattc tcaagtggat
      361 cgtcatcgtt gtcgtgttag tgatcgtcgt cggcctggcg ctggtttact tcaacatcga
      421 ccgcatcgtg aagcacacgg tcgagacaca gggcaccgac cagctgaagg tccctaccac
      481 cctggacggc gtcagcctcg ggctcttcaa gggcacggtc gcgctgaacg acctgaacgt
      541 cggctcgccc cccggcttca ccgcgccgca catgctgacg ctgggtggat tgaacgtaga
      601 caccggcggc atctcgaacc tgcgcaagga gccgatccac gtcagcagca tcgcggtcga
      661 tcaacccaag ctggtggtcg aattcgccgg cacggaagtg aacttcaaga agctgatgga
      721 caacctgccg agcaacccgg accagaaccc cccgccgagc gagaagaaac cgacgaagct
      781 gattatcgac gacctgacgg tcaacaacgc gcacgtcgtg ctgcgcgggt tggacacagt
      841 ggccggaaaa atccccgggc tcagcaaagt ggcgaacctg ccggcggagc tggacctgcc
      901 gatcccgtcg atcgacgtga agaacatcgg caacgccgac ggcgcgcaga acggcgcggc
      961 catcaaagac gtggtgacga cgatcatcac ccaaatggcc gccaaggccg ccgattcgga
     1021 taaggttcca ccggaagtaa aagccgtgct gaccggcaat ctcgatgcgg tgaaggacaa
     1081 gctcaccggc atggccaccg cggaactgaa caaggtgacc gaccagctga aggctaaatt
     1141 gcccgcgggc attccgacca gtctccccgc gaacctcccc ggcaaccttg gcggcctcat
     1201 caacaaagcc accggcgcta acggcaacgc acccgccgat cagaacggcg gcaacaaccc
     1261 ccccagcccc gcggataacc tgctgaacca gggcctgggc gcgttgcgcg gcgcgacgtc
     1321 gaaacccgcg aagtaagcga gcgtttacag ccaaccggag cccgcgtacg ctccgaaccg
     1381 ccgtgggtgg taatgacgat gatcaacaga tcgaatgcta agcgctcatg tcgagatagc
     1441 gccggtagcg ggccagcgcc atcagcggga agtagtgccg atagaggtga tacttcaaat
     1501 agaacacccg cgggaaaccg gtgccggtga accattgttc gtcccacgcg ccgtcggcgg
     1561 cctggtgggt gatgagccag tcgatgccgc ttttcaccgc cggatcgtgc gggccggcgg
     1621 cggccagcag cgccatcgtg ccccacgcgg tttgggatgc ggtgctttcg ccctggccct
     1681 tcagactcgg atcatcgtag ctggcgcatg attcgccgaa gctgccgtcc ggcttttgaa
     1741 cgcttcgtaa ccacgcgatc gcgcgctgga tgtattcggc gttcatgtct tcgccgaccg
     1801 cgcgcaagcc cgcgaggacc tgccaggtgc cgtagacgaa gttgacgccc caccggcccc
     1861 accacggccc atccttatcc tgctcgcggc ggatgaaatg gatcgcgcga tcgacgatcg
     1921 tctgcccttt gcgaaagccg ttgtgcccga gcgattccag cacgcggccg gcgatgtccg
     1981 ggcacgacgg gtcctgcatc gcattgtgat cggcgaatgg gatgtactcc aggatcggcc
     2041 ggctcttggt cttgtcgaac gcggcccacc cgccgtcact gttttgaaac gccagcagcc
     2101 aattcaaccc gcgtcggacc gccggttcgg cctttttgcc gcccaatcgc ttcagcgaca
     2161 tcaccgccat ggcggtgtcg tcgacgtcag ggtaatgagg attgtgaaac tcgaagaacc
     2221 aaccactcgg ctcaaccggg cgcgcgttgc gctgccagtc ggaggcgtat ttgcattcct
     2281 tgtccagcag ccactgcgtg ccgcgcatcg cggcgtcgga atcgattgat aaccccgtct
     2341 ccgccagcgc gtgcatcgcg atgccggtgt cccacaccgg cgagacgcac ggctgaaggc
     2401 gaatcgttcc ccctcgcccc ggtacgcagg ggagagggcc ggggtgaggg gcctcctccc
     2461 gaatataaaa atccttaaga tccttatgtg ccttcaacac gaccggatga tcatccggat
     2521 accccatcgc cttgaagacg atcagcatgt agaccatcgg cgggaagatc gcgcccaggc
     2581 cttcggaatc ggccatgtga tccagcagcc atttttccgc cgcctgcatc gcgcgctcgc
     2641 gcaggcccgg gaacggcgcc tcttcgtatc gcttcagcag ccgatcgacg cgcaggaaaa
     2701 agtcgcgcca cgtgcgcgga aaacccggtt tgggaatgcc cagcatattg ccggcggcgt
     2761 gatcgacgta cagttcggcg atgcccagct ccgccggcac gtcgcgcacg cggcggagcg
     2821 tcgtgacgat gcccagcggc aggatcatcg tgcgcgtcca cgccgacacg ttgtacaggt
     2881 tgaagtaaaa ccacttgggc agaaacacga cttccggcgg aatgctggga cacgaatcga
     2941 aactgatctg tcctaagcag gcaaaataaa accgtgtgaa cgtgttgcac ttttccgccc
     3001 cgcccaggct gcggatcaac tcgcgcgcct tggtcatgtg cggc
//
LOCUS       DATIMH010000048         5452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_876191, whole genome shotgun sequence.
ACCESSION   DATIMH010000048 DATIMH010000000
VERSION     DATIMH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876191"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VLI90_00820"
     CDS             <1..770
                     /locus_tag="VLI90_00820"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSV12770.1"
                     /translation="GQRVVGEINIVCGRCDLCLSGLSSHCRSRSVLGILNHDGAFAEF
                     VRLPAVNLHVLPKAVDDDQAVFVEPLAAAFQVLKQLGTQGRSSTAEGRRWVTVLGDGR
                     LGLLVAQVMRNAGYPVRVIGKHPDKLAICEKWSIRSRPLADIVPRHDQDIVVDCTGSA
                     AGFELAMQMVRPRGTIVLKSTVAAGKPLNLAPLVIDEISVIGSRCGPFREAIRALAEK
                     QVDVASLITRRMKLEQGVEAMELAARPGVLKVLLTME"
     gene            906..1565
                     /locus_tag="VLI90_00825"
     CDS             906..1565
                     /locus_tag="VLI90_00825"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HSV12771.1"
                     /translation="MAKDLAIVLNNGSVNSAVATALAAQKYRTILLHAEAGEHPGPRA
                     RAAYDLQVAHFKPYREHTLPMPYLSLIQPANERSMGASDPRHPTPLAPQMVDLLPLIS
                     AALRFAAHYQAAAVYLGLRVGPSPDELAQATEYTQIWNELIQMPCNQPEMTVEMPLLE
                     LEPWQVVDVGFQISAPFDRTWSCLEGGSEPCWACKGCRSREAAFQQSGKADPLRVVRK
                     V"
     gene            1827..1955
                     /locus_tag="VLI90_00830"
     CDS             1827..1955
                     /locus_tag="VLI90_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12772.1"
                     /translation="MSLKPGDVINNCTLFNHVICKIELWWDRPDALRGDKCSVGLN"
     gene            complement(2348..2803)
                     /locus_tag="VLI90_00835"
     CDS             complement(2348..2803)
                     /locus_tag="VLI90_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12773.1"
                     /translation="MPEIDINHLLWIVVGVQLSAELGDRPLAYRIEQQLKDRLDKTLG
                     PPAAGELPKLAPAVISDVYYLNSEEAQSRPVISIGGPGVNALSAQLVNDLPTVIAIED
                     KLVVQMDLEMADLRVAVWGMNHVETVKAVELFVDRGYLDAFVTGVLSQS"
     gene            complement(2821..4896)
                     /locus_tag="VLI90_00840"
     CDS             complement(2821..4896)
                     /locus_tag="VLI90_00840"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSV12774.1"
                     /translation="MQDLQQQAAALRSPSSSIARREEAAHRLLSRPSPQAFDVLRGAL
                     QTTDNPTAQIAVARALGDDPNANPVFIPLLIGAIGPNRALSEAAAEALANYPGNNQAA
                     GALQNVAENARLPETTRTNIVRAMGGMIDKATADYLVNLMNSRTGPMQAAAIEALARM
                     SGNPTASDDPQRLQQWWNVNANKSEEAFRADLLAARAAEANRLRLRHAQLTDDLRTLL
                     ADQYRMLSDPRDVETRKVETLFNLLNSRDPAVRAVGPEIVLQDFQNNRPIDPAIRARL
                     RAMVGDSDSNVRREVVNTITEINDRDAAKAFITQLRQESVPDIRTKLARALGRMGDPQ
                     AVTLLLEMLQSQNTAEATLAANALGEMGDKLRQTNPGAAQQVGDALRKIIDATATSRD
                     ATGLRAACVAALGALHDPRAVDTFLPLLRPNVDSTIRKAALHGLASLGDPNTAEPIAL
                     LPLIDPDASIRLEAARALQNIATFAQSEQLYRMLDPREEPNAQVRDAVWDDLNRVLHS
                     GTTQQLNGWPDRFKNDPPKRLETLYALRDSLIKDGNQEQLAFTRQNIAAELMNLDPKQ
                     TDEAADNIQKALDYWRGEGHSKIGSETTLDGLVGQMLSVLFEAKKYDDAAAFAAKQIE
                     INRQYQETVGSRIKQEADRLHAAGRNSDATALIDAALRMSPPLDSKYQRDLRDIRAEA
                     VKPSPVPGP"
     gene            4895..5164
                     /locus_tag="VLI90_00845"
     CDS             4895..5164
                     /locus_tag="VLI90_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12775.1"
                     /translation="MPSFAGELVGEFAGKVEGAGAASAAKQGGATAAVIAAAVSNAPA
                     ATLKFPRTVCSFRLPIDTPQLHGAHFDVVRAATTAARARACARRR"
BASE COUNT          950 a         1715 c         1768 g         1019 t
ORIGIN      
        1 tgggccagcg cgtcgtcggc gagatcaaca tcgtctgcgg ccgctgcgac ctgtgcctgt
       61 cgggcttgag cagccactgc cgcagtcgca gcgtgctggg gattttgaat cacgacggcg
      121 cgttcgccga attcgttcga ctgcccgcgg tgaatctgca cgtgctgccg aaggcggtgg
      181 atgacgatca agcggtgttc gtcgagccgc tggcggcggc gtttcaggtg ctgaagcaac
      241 tcggcacgca gggccgcagc agcaccgccg aaggccggcg gtgggttacc gtgttgggcg
      301 atggccggtt gggtttgctc gtcgcgcagg tgatgcgaaa cgccggttat cccgtgcgcg
      361 tgatcggcaa gcacccggac aagctggcga tctgcgagaa gtggtcgatc cgctcgcggc
      421 cgctggccga catcgtgccg cggcacgacc aggacatcgt cgttgattgc accggcagcg
      481 ccgcgggatt cgaattggcg atgcagatgg tccgtccgcg cgggaccatc gtgctcaaga
      541 gcacggtcgc cgcgggcaag ccgctgaatc tcgcgccgct ggtgatcgat gagatcagcg
      601 tcatcggcag ccgctgcggg ccatttcgcg aagcgatccg ggcgctggcg gaaaagcagg
      661 ttgacgtcgc gagtctcatc acgcgccgga tgaagctcga gcagggcgtg gaggcgatgg
      721 agctcgctgc aagaccgggc gtgttgaagg tgctgttgac gatggagtga cgccgtggca
      781 cgggctagca gccgtgtttc gagcgcagcg ttacgattcg cgagaacaac aagagccgct
      841 atagcggcaa gtcatgggca ggttgcccat gctacggatg tgggcgaata gcccatgcta
      901 agaatatggc caaagacctg gcgatcgtcc tgaacaacgg cagcgtgaac agcgcggtgg
      961 cgacggcgct ggcggcgcag aagtaccgca cgatcctgct gcatgccgag gccggcgaac
     1021 atccgggtcc gcgcgcgcgg gccgcgtacg atctgcaggt tgcgcacttc aagccgtatc
     1081 gcgagcacac gctgccgatg ccgtatctca gtttgattca acccgccaac gagcggtcga
     1141 tgggcgcgag tgatccgcgg catcccacgc cgctcgcacc gcagatggtg gacctgctcc
     1201 cgctgatctc cgccgcgtta cgcttcgccg cccattacca ggcggccgcg gtttatttgg
     1261 gccttcgcgt cggccccagc ccggatgagt tggcgcaggc gacggaatac acgcaaatct
     1321 ggaatgagct gatccagatg ccgtgcaatc agccggagat gacggtcgaa atgccactgc
     1381 tggaactgga gccgtggcag gtggtggatg tcggttttca aatctccgcg ccgttcgatc
     1441 gaacgtggag ctgcctggaa ggcggcagcg aaccgtgctg ggcgtgcaag ggatgccgat
     1501 cccgcgaggc ggcgtttcaa cagtccggaa aggcggaccc gctgcgggtg gtgcggaagg
     1561 tttagtttcc cttgtcctga gcgaagcacg tacgcgtggg cagtcgaagg atcgagccga
     1621 gaagtgagcg aagcgctcga gcgcaagcac gtcagaacga ggcgtcattt ttgagcaaac
     1681 gcggctcgat ccccatagtc ccaatctatt tccctccgct tccgccattc cgcaacaagc
     1741 cgccgatctg gcgcgggctt ccaaaacgcc ttggcaaaaa atacgccggc gacgatgact
     1801 ggctccgacg acatcgccag tggttgatgt cattgaagcc cggcgacgtg atcaacaact
     1861 gtacgctgtt caaccacgtc atctgcaaaa tcgaactgtg gtgggaccgc cccgacgcgc
     1921 tgcgcgggga caagtgctcc gttggattga actgacaaca gcgggaagcg gcgattgcag
     1981 tggattccat tgtgggatac tccccgcact cgatcagcgt caattgcttg cgcagtgcca
     2041 aagctgcggc tgggtggagg atagctggct tcacgagttg atccgacgca tgcagcgcgg
     2101 cgatcgagtg ctcgacgaac atcgcctgct tgtgccgcgc gagggcgatc cagagaacat
     2161 ccatcgcgcg tacgaggcga acgtgaaagc gctgaaagaa ttttgcaacc ggcgttagcg
     2221 ctcacgatct atcagatgcg aaaagagcac aggagccccg cgtagcggta gccccgaagg
     2281 ggcacgtgtt cacgttcgag cgccgaaaca cgtgcccctt cggggctacc gctacgcggg
     2341 cagaaattca cgattgcgaa agtacccccg tcacaaacgc atccaaatat ccccggtcca
     2401 caaacagctc caccgccttc acggtttcga cgtgattcat cccccacacc gcgacgcgta
     2461 ggtccgccat ctccaggtcc atctgcacga ccagcttgtc ctcaatcgca atgaccgtcg
     2521 gcagatcatt caccagttgc gccgacagcg cattcacacc cggcccgccg atgctgatga
     2581 ccggacgcga ttgcgcttcc tcgctgttca agtaatacac atcgctgatc acggccggcg
     2641 ctagcttcgg cagctcaccg gcggcggggg ggccgagcgt tttgtcgagc cggtctttca
     2701 actgctgctc gatgcgatac gccaacgggc ggtcgcccag ttccgcgctc agctgaacgc
     2761 cgaccacgat ccacagcaga tgattgatgt cgatctcggg catcgtaatg gccggtcatt
     2821 ttacgggccc ggcaccgggg agggcttcac ggcctcggcg cggatgtcgc gcaagtcgcg
     2881 ctgatatttc gaatccagcg gtggcgacat gcgcagggct gcgtcgatca acgcggtcgc
     2941 atcgctgttt cgtcccgcgg catgcaggcg gtccgcctcc tgcttgatgc gcgacccgac
     3001 cgtctcctga tactgccggt tgatctcgat ctgcttcgcc gcgaacgccg cggcgtcgtc
     3061 gtatttcttt gcttcgaaca acaccgacag catctgaccg accagcccgt ccagcgtggt
     3121 ttcgctgccg atcttgctgt ggccttctcc tcgccagtag tccagcgcct tctgaatgtt
     3181 gtcggccgcc tcgtcggtct gcttggggtc gaggttcatc agctccgccg cgatgttctg
     3241 ccgcgtgaat gccagctgct cctgattgcc gtctttgatg agcgaatcac gcagcgcgta
     3301 cagcgtttcg agccgcttgg gcggatcgtt cttgaaccga tcgggccagc cgttcagttg
     3361 ctgcgtcgtg cccgaatgca gaacgcggtt caggtcatcc cacaccgcat cgcgaacctg
     3421 tgcgttcggt tcctcacgcg gatcgagcat gcgataaagc tgctccgact gcgcgaaggt
     3481 ggcgatgttc tgcagcgccc gggcggcctc aagccgaata ctcgcatcgg gatcgatcag
     3541 cggcaacagc gcgatcggct cggccgtgtt cggatcgcca aggctcgcca agccgtgcag
     3601 cgctgctttg cgaatggtgc tatcgacgtt cggtcgcagc agcggaagga aggtatcaac
     3661 cgcgcgcggg tcgtgcagcg cgcccagggc cgccacgcac gcggcgcgca ggccggttgc
     3721 gtcgcgactg gtcgcggtgg cgtcgatgat ctttcgcagc gcatcaccca cctgctgcgc
     3781 cgcgccgggg ttggtctgcc gcagcttgtc gcccatctcg cccagcgcgt tggcggccag
     3841 cgtcgcctcg gcggtgttct gcgattgcaa catctccagc agcagcgtca ccgcctgcgg
     3901 gtcgcccatc cggcccagcg cgcgggccag cttcgtgcgg atgtccggca cactctcctg
     3961 ccgcagctgc gtgatgaacg ccttggcggc gtcgcgatcg ttgatttcgg tgatcgtgtt
     4021 gaccacctcg cgccgcacgt tcgaatcgct gtcgccgacc atcgcgcgca gtcgcgcgcg
     4081 gatcgccggg tcgatcggac gattgttctg aaagtcctgc agcacgattt ccggccccac
     4141 cgcgcgcacg gccgggtcac gcgagttgag caggttgaac aacgtttcga ccttccgcgt
     4201 ctcgacgtcg cgcggatcgg acaacatgcg gtactgatcc gccagcaacg tgcgaaggtc
     4261 atcggttagc tgcgcatgcc gcagacgaag gcggtttgcc tcggcggcgc gagcggcgag
     4321 taaatcggcg cgaaacgcct cttcactttt gttggcgttc acgttccacc actgctgcag
     4381 gcgctgcgga tcgtcgctgg cggtcgggtt gccgctcatt cgcgccagcg cttcgatcgc
     4441 ggccgcctgc atcgggccgg tgcgcgagtt catcaggttg accaggtaat ccgccgtcgc
     4501 cttgtcgatc atcccgccca tggcgcggac gatgttggtc cgcgtggtct cgggaagccg
     4561 ggcgttttcc gcgacatttt gcagcgcgcc tgccgcctgg ttgttgccgg gataattcgc
     4621 cagcgcctcg gcggcggctt cgctgagcgc gcggttgggg ccgatcgcgc caatcaataa
     4681 ggggatgaag accggattgg cgttgggatc atcgcccagc gctcgcgcga ccgcgatttg
     4741 cgcggtgggg ttgtcggtcg tttgcagcgc gccgcgcaaa acgtcgaacg cctgcggcga
     4801 cgggcgactc aacaatcgat gggccgcctc ttcccggcgg gcgatggaac tggatggact
     4861 tcgcagcgcc gcggcctgct gctgcaggtc ctgcatgccg tcatttgccg gtgaattagt
     4921 gggcgaattt gccggtaaag tggagggggc gggagcggcg tcggcggcaa aacagggcgg
     4981 cgcgaccgcg gcggtcatcg ccgccgccgt cagcaacgca cctgccgcaa ccctgaagtt
     5041 cccgcgcacc gtctgttctt tccgccttcc gatcgacacg ccgcagttgc acggcgcgca
     5101 cttcgatgtc gtccgagccg cgaccactgc cgcgcgcgca cgcgcgtgcg cgcgccgaag
     5161 gtaacccatt tccagtccgg aacaaagcat tcagcgcaac ggccccactt gcggacgcgc
     5221 cgcgcgttcg cttaaatgtc tgcatcgcat ttgtgttggt tgtccgttca atttagcgaa
     5281 gttctccgcg ccgcaaaacg cgccccgcgg agctggtggc catgagcgac tggaacgaag
     5341 ccgaacagcg agtcgagaag gctcaggaac tgttcgaaca gcatcgttgg caggaagccc
     5401 ttgatgaact tcaggcggca accgccatca atccatacaa cggcgggtgg tt
//
LOCUS       DATIMH010000049         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_10386, whole genome shotgun sequence.
ACCESSION   DATIMH010000049 DATIMH010000000
VERSION     DATIMH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10386"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VLI90_00850"
     CDS             <1..710
                     /locus_tag="VLI90_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12776.1"
                     /translation="IHVIRPEVVEKAAMKPVMGRGKVFVIEQAELMNPPAQNALLKTL
                     EEPAGRALIILLTDQPGLLLPTIRSRSQIVRFAALDDATVLRELQKRGIAKTDAADAA
                     RFAAGSLGVALKWFEDGVIDPVRQLMRQLDDIFAGKPAGELADWFKAAADAYAEKQLE
                     RDELGSKDQATREGLTLYLKLAADHVRQRLAELDEPDELERSCAAIDAIARAEEYLDA
                     NVNIPLVFQQLAVALER"
     gene            897..>2201
                     /locus_tag="VLI90_00855"
     CDS             897..>2201
                     /locus_tag="VLI90_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12777.1"
                     /translation="MMESAAAEAAGVVADGGGNAAPPARVMRSLLCASIVFIIAFGVV
                     FFRTPWPGTTIGDSDAGHQLAGGQQIAFGEHPFVDFRSTYGPLTFYVSFIGQWLSGGR
                     LAGELLICTLAYAFVYALLLVMSRWMTRGGRVLPIAITVLALIQLPRFYKYYILLGPT
                     LALFAFLWYLARPTWPRRLFAAFGVVVAGLYRPDMGAYAALAGMVAMALVQRDWDTRV
                     RDMFRFGVLIVLVASPWLVFVSARHHLLDYLHDSVMGSASQAQGLSLPFPRPHADLPW
                     NAPANLEASAFYIWWSLPIIAAIITAMRWRAMDELLRRRAVVTIILAALCLLQSAHRS
                     EYNHLMQALAPSYVLAAFVVAQLIDATRRMPGRIAIASLAVVLGGVSVAAGKATGALA
                     GGAVKAISSYRNFYLRDRQAFVARVGETYPKNPYPKLIRYIDD"
BASE COUNT          342 a          661 c          781 g          417 t
ORIGIN      
        1 cgatccacgt gatccgtccc gaggtagtcg agaaggcggc gatgaaaccg gtgatgggcc
       61 gcggcaaggt gttcgtgatc gagcaggcgg agctgatgaa cccgccggcg cagaacgcgc
      121 tgttgaagac gctggaagaa ccggcggggc gggcgctgat tattctgctg acggaccagc
      181 cggggctgct gctgccgacg attcgcagcc gatcgcagat cgtgcgcttc gcggcgctcg
      241 atgatgcgac ggtgctgcgc gagttgcaga agcgcgggat cgccaagacc gacgccgcgg
      301 atgcggcgcg attcgccgcg gggtcgctcg gcgttgcgct gaagtggttc gaagatggcg
      361 tgattgatcc ggtccgccaa ctgatgcgac agctggacga catcttcgcc ggtaaacccg
      421 cgggggagct agccgactgg ttcaaagccg ccgccgatgc gtacgcggaa aagcagctgg
      481 agcgcgacga gctgggcagc aaggaccagg cgacgcgcga agggctgacg ctgtacctga
      541 agctggcggc ggatcacgtg cggcagcgat tggccgagct cgatgagcca gacgaattgg
      601 aacgatcgtg tgccgcgatc gacgcgatcg cccgggcgga agaatatctg gacgcgaacg
      661 tgaacattcc gctggtgttt cagcagctgg cggtggcgct ggagcgatga tctgttcccc
      721 tcgcccaggt attcgtggga gagggttagg gtgagggtga tttagaattt cgagagccgt
      781 tggtactcga aaccaccctc acccccgccc tctcccacga gtacatgggc gagggggtaa
      841 gagctcgtcc actccgtcgc tggcggtgtc ccgccgcacc gatatacttc ggccgcatga
      901 tggaatcggc ggctgctgag gcagcgggtg tggttgcgga tggcgggggg aacgcggcgc
      961 cgccggcgcg cgtgatgcga tcgctgctgt gcgcgtcgat cgtcttcatc atcgcattcg
     1021 gcgtcgtctt ttttcgcacg ccatggccgg ggacgacgat tggcgacagt gacgccggcc
     1081 accagctggc cggcgggcag cagatcgcct ttggcgaaca tccgttcgtc gactttcgca
     1141 gcacctacgg cccgctgacg ttctacgtct cgttcatcgg tcaatggctc agcggcggcc
     1201 ggctcgcggg cgaactgctg atctgcacgc tggcctacgc cttcgtttac gcgctgctgc
     1261 tggtgatgtc gcgatggatg acccgcggcg ggcgagtgct gccgatcgcg atcacggtgc
     1321 tcgcgctcat ccagctgccg cggttttaca aatattacat cctgctcggg ccgacgctgg
     1381 cgctgttcgc gttcctgtgg tatctggcac ggccgacatg gccgcggcgg ttgttcgcgg
     1441 cgttcggtgt cgtcgtcgcg gggctctatc gaccggacat gggtgcgtat gccgcgctgg
     1501 ccggcatggt cgcgatggcg ctcgtgcagc gcgactggga cacgcgcgtg cgcgacatgt
     1561 ttcgcttcgg tgtgttgatc gtgctcgtcg cgtcgccatg gctcgtgttc gtcagcgctc
     1621 ggcatcacct gctggactac ctgcacgatt cggtgatggg atcggccagc caggcgcagg
     1681 gcctgtcgct gccgttcccg cgcccgcacg cggatctgcc gtggaacgcg ccggcgaacc
     1741 tggaagcgtc cgcgttttac atctggtggt cgctgccgat catcgcggcg atcatcaccg
     1801 ccatgcgctg gcgcgcgatg gatgaactgc tgcgacgacg tgcggtggtc acgatcatcc
     1861 tggccgcgct gtgcctgctg cagtcggcgc atcgatcgga atataaccac ctgatgcagg
     1921 ccctggcgcc atcgtacgtg ctggcggcgt tcgtcgtcgc tcagctgatc gacgcgacgc
     1981 gccggatgcc cgggcgcatc gcaatcgcct cgcttgccgt tgtgctcggc ggcgtgagcg
     2041 tcgcggcggg gaaggccacg ggcgcgctgg cgggtggagc ggtcaaggcg atttcgagct
     2101 accggaactt ctacctgcgc gatcggcagg cgttcgtcgc gcgcgtgggt gaaacgtatc
     2161 cgaagaaccc atatccgaag ctgattcggt acatagacga t
//
LOCUS       DATIMH010000050         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1743123, whole genome shotgun sequence.
ACCESSION   DATIMH010000050 DATIMH010000000
VERSION     DATIMH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743123"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VLI90_00860"
     CDS             <1..252
                     /locus_tag="VLI90_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HSV12778.1"
                     /translation="MSLIGPRPLTLREIREYYGDVADEMLQLKPGIAGLWQISGRNRL
                     TYDERRRMDLDFVRRRSLKMYLRIAWRTLPEIFSGANSW"
     gene            279..1298
                     /locus_tag="VLI90_00865"
     CDS             279..1298
                     /locus_tag="VLI90_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HSV12779.1"
                     /translation="MNRLTRRLVLILLAIVTTLLIGTVGFTVIERYPPFDAFYMTLTT
                     MTTVGYMEIHPLSQAGRVFNSFLIFFGVTTIFIAIGAMTQTIIELEFGDAIGKRRNKR
                     MIDKLKDHFIICGFGRVGRGAAAELQHAGVPFVVVDINPERVERAMLAGMLAANADST
                     RDETLRLVGIEKARGLVAALATDADNLFVLLSAKGLNPGIYVAARAAEEGAEEKMRRA
                     GADAVFAPYSITGHRLAQSLLRPHVVQFLDFTTKAIGLDIAIEQIRVSESSEMASKSI
                     KDLQLGRDFGVIVMAIRTSDGQMHFNPTAESTVQAGDHLIVMGSQEKLRALEVLIAEA
                     RPTRR"
     gene            1572..>2152
                     /locus_tag="VLI90_00870"
     CDS             1572..>2152
                     /locus_tag="VLI90_00870"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HSV12780.1"
                     /translation="MIYIAKRERIEPELVRSEVARGRMIIPANVNHTNLEPMCIGVAS
                     KCKINSNIGNSAVTSDIEGELEKLAYSVKYGADTVMDLSTGGDIPAIRKAIIGASPVP
                     IGTVPIYEALSRVRRVEDLSKQVMLEVIEEQAEQGVDYMTIHAGVLVQYIPLTTKRIT
                     GIVSRGGSILAEWMVKNHKQNMLYECFEDICKI"
BASE COUNT          451 a          632 c          681 g          388 t
ORIGIN      
        1 atgtcgctga tcggaccgcg tccgctgacg cttcgcgaaa ttcgcgagta ttacggcgac
       61 gttgccgacg aaatgctgca gctcaagccc gggatcgccg gcctgtggca gatctcggga
      121 cgcaaccggc tgacgtatga cgagcggcgc cggatggatc tcgatttcgt acgccgccgg
      181 tccttgaaga tgtacctgcg aatcgcgtgg cggaccttgc ccgagatttt cagcggcgcg
      241 aatagctggt agctggcggg cgctatactt gtcgttcgat gaaccgcctg acgcgccgtc
      301 ttgtcctcat tctcctggcg atcgtcacca cgctcctcat cggcaccgtc gggttcacgg
      361 tcatcgagcg ctaccccccg ttcgacgcct tctacatgac cctgacgacc atgacgacgg
      421 tcgggtacat ggagatccat cccctgtcgc aggcagggcg cgtcttcaac tcctttctga
      481 tcttcttcgg cgtcaccacc atcttcatcg ccatcggggc gatgacccag accatcatcg
      541 agctggaatt cggggacgcc atcggcaaac ggcggaacaa acgtatgatc gacaagctca
      601 aggatcactt catcatctgc ggcttcgggc gcgtcgggcg cggcgccgcg gcggagctgc
      661 agcacgccgg cgtgccgttc gtcgtcgtgg acatcaatcc ggaacgcgtg gagcgcgcca
      721 tgctggccgg gatgctcgcg gccaatgcgg actccacccg cgacgaaacg ctgcgcctgg
      781 tcggcattga aaaggcgcgg gggctggtag cggcgctcgc gaccgacgcc gataaccttt
      841 tcgtcctgct ctcggccaag ggcctcaatc ctggtattta tgtcgcggcg cgtgccgccg
      901 aagagggcgc ggaagagaag atgaggaggg cgggcgccga cgccgtgttc gcgccgtatt
      961 cgatcacggg gcaccgcctc gcgcagtcgc tgctccggcc gcacgtggtc cagttcctcg
     1021 acttcaccac caaagcgatc gggctcgaca tcgcaattga gcagattcgg gtgtcggaat
     1081 ctagcgaaat ggcgtcaaaa agcatcaaag atctgcaact tggacgcgat tttggggtaa
     1141 ttgtgatggc aattcgcacc agcgacggcc agatgcactt caatccgacg gcggaatcga
     1201 ccgtacaggc gggcgatcac ctcatcgtga tggggagtca ggagaagttg cgcgcgctcg
     1261 aagtgctgat cgcggaagcc cggcccaccc gcagataaat acggggtggg aagtccgtaa
     1321 gcggttataa tcgcatcgta agggaggatc ttgtggccaa ggacagaagc ccgaaaaaag
     1381 agaccaagaa gccgaagaag aaaaagtagg ttcccccagg cacacgtaga cgggccgggt
     1441 ttgcgttggg agtagtaccc ggcccctgca ctcaggagtc agtcatgcgc acagagtggg
     1501 tagcaaaacg gaaaaacgac agcaaccgca cgcagatgca ctacgcccgg cggggcatga
     1561 tcacggagga gatgatctac atcgccaagc gcgagaggat cgagccggaa ctggttcgct
     1621 ccgaggtggc gcgcgggcgg atgatcattc ccgccaacgt gaatcatacg aatctcgagc
     1681 ccatgtgcat cggggtcgcg tccaagtgca agatcaactc caatatcggc aactcggccg
     1741 tcacttcgga cattgaaggg gagctcgaga agctggccta ctccgtgaag tacggcgccg
     1801 acacggtgat ggacctctcc acgggcggcg atattccggc cattcgcaag gcgatcatcg
     1861 gggcctcgcc ggtgccgatc ggcacggtgc cgatctatga agcactctct cgcgtgcgcc
     1921 gtgtggagga cctgagcaag caggtgatgc tggaagtcat cgaggagcag gcggagcagg
     1981 gggtcgatta catgaccatc cacgccggcg tgctcgtgca gtacatcccg ctcaccacta
     2041 agcgcattac gggcatcgtc agtcgcggcg ggtcgatcct ggccgagtgg atggtgaaga
     2101 atcacaagca gaacatgctg tacgagtgct ttgaggatat ctgcaagatc tt
//
LOCUS       DATIMH010000051         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4053113, whole genome shotgun sequence.
ACCESSION   DATIMH010000051 DATIMH010000000
VERSION     DATIMH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4053113"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VLI90_00875"
     CDS             complement(<1..1174)
                     /locus_tag="VLI90_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HSV12781.1"
                     /translation="MSILRLLAMTLLLASSTFAADEAPATAPSVPKGEVTKHVFDPSK
                     IFPGTMRDYWVYVPKQYDGSKPACLYVDQDNVQFKSPGVFDQLIASGEMPVTIGVYVA
                     PGVVKAPSDAALDRYNRSYEFDGMSDEYARFLIEELLPHVIADQKLNISNDPNDRCIA
                     GSSSGAIAAFTAAWERNDSFHRVFSAIGTYVDLRGGNIYPSLIRKFEPKTIRIFLQDG
                     SNDNNHYGGDWWMANNEMQRALTFAGYEVNHAWGTGKHEHTQADAVFADAMRWIWKDW
                     PAPVKTGLGSEKLKEVLVPGEGWQMVGTAGGRDDGPAANAAGEVFINDGMQTMKIGLD
                     GKITPYAKVKTGGQAFGPDGKLYIASGSTGEILVCDGDGHTTVVTDGIKANDLVVAH"
     gene            1292..2608
                     /locus_tag="VLI90_00880"
     CDS             1292..2608
                     /locus_tag="VLI90_00880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HSV12782.1"
                     /translation="MTGTDSPRAILLRRLFLALFCIIAAVFCFAPIRNQLRGGSTKDY
                     PLWFDTGQRVLHGDSPYYKDRHNEFPFMYPPGASVLLAPITVFGKLPMMIVLVAINTF
                     AWVVCILAPIYLVAGTTRQQPLILYVLPTAVCIVYIWSSYLLGNPPIVLAACVLGMFI
                     CLRKRIDWSAGLLLALAAGFKAFPILAVPYLMWRRHWRALGYTVAFLFILLLVLPALF
                     RGVHGAAADLNLWTHDTLGSYNGEQIGQRKQRSYLWKNGSIFAEAHRLLRSGVVADYD
                     DNSPEEPALTVNVASLSFTQVNLVILIATAILCGSYLLVMPRGANRTPWSDAIEGAML
                     MILIITYTPLSFTYNNSWLMLPIVVVLYFICCKARSATEKRLAVAWLAIALLLLIFTA
                     GKIPFFRYMRSLGNTFWCCMLLYAELIWIMLQARRESNVLQPEQSV"
     gene            complement(2585..>3147)
                     /locus_tag="VLI90_00885"
     CDS             complement(2585..>3147)
                     /locus_tag="VLI90_00885"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: protein motif:HMM:NF016857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HSV12783.1"
                     /translation="FHNDVVAVSNERVLLCHALAFAGNALAEIRQSLSWEPTIIAAAE
                     AELSLADAVQSYVFNSQLVTLPDGAMALIAPVECRDHPRVQAFLQRVLAEDNPIRAVH
                     YLDVRQSMFNGGGPACLRLRVVLTDEQLRAVHAGVMLDEALYAKLVASVKRHYREMLS
                     AADLADPVLMRETAAALADLNGLLGL"
BASE COUNT          546 a         1052 c          901 g          648 t
ORIGIN      
        1 cgtgcgcgac gacgaggtcg ttcgccttga tgccgtcggt gacgaccgtg gtgtgaccat
       61 cgccgtcgca gacgaggatc tcaccggttg aaccggacgc gatgtacaac tttccatccg
      121 gcccgaacgc ctgcccgccc gttttcactt ttgcgtatgg agtgatcttg ccgtcgaggc
      181 cgatcttcat cgtctgcatg ccgtcattga tgaagacctc gccggccgcg ttggccgcgg
      241 ggccgtcgtc gcgcccgccg gcggtgccga ccatctgcca gccttcgccg ggaactaaaa
      301 cctctttcag cttttccgac cccagccccg tcttcaccgg cgccggccaa tctttccaga
      361 tccatcgcat cgcatcggca aacaccgcgt ccgcctgcgt gtgctcgtgc tttccggtgc
      421 cccaggcgtg gttcacttca tagccggcaa acgtcagcgc gcgttgcatc tcattgttcg
      481 ccatccacca atcaccgccg taatggttgt tgtcgttgct gccgtcttgg agaaagatgc
      541 ggatcgtctt cggctcgaac ttgcggataa ggctcgggta aatatttccg ccgcgaagat
      601 cgacgtaggt gccgatcgcg ctgaagacgc ggtggaagga atcgttccgc tcccacgccg
      661 ccgtgaacgc cgcgatcgcc ccgctgctgg acccggcaat gcagcggtcg ttcgggtcgt
      721 tcgaaatgtt cagcttctga tccgcaatga cgtgcggcag cagttcctcg atcaaaaagc
      781 gcgcgtactc atcgctcatg ccgtcgaatt cgtaactgcg gttgtagcga tccagcgccg
      841 catccgacgg cgccttcacg acgccgggcg cgacgtagac gccgatcgtc accggcatct
      901 cgccgctggc gatcagctgg tcgaacacac ccggcgactt gaactgcacg ttgtcctgat
      961 cgacgtagag gcacgcgggc ttcgacccgt cgtattgctt gggaacgtag acccagtaat
     1021 cgcgcatcgt gccggggaag atcttgctcg ggtcgaagac gtgcttcgtg acctcacctt
     1081 ttgggacgga cggcgcggtc gccggcgctt cgtctgcagc gaatgtagaa gatgcgagga
     1141 ggagcgtcat cgccagaagc cgaaggatag acatgctctt ctcccgtccc gccgcttgcg
     1201 gcttcgcaag ccacatcgcc ccgattgcca agccgcgagc ggcgggaagt tggatttcaa
     1261 atactctcga tgctacaaat cgcggcccat tatgaccgga acggattcgc cccgcgccat
     1321 cctgcttcgc cgcctgttcc tggcgctgtt ctgcatcatc gccgccgtct tctgcttcgc
     1381 gcccattcgc aaccagctgc gcggcggcag cacgaaagat tatccgctgt ggttcgacac
     1441 cggccagcgc gtgctgcacg gcgacagccc gtattacaaa gatcgccaca acgagttccc
     1501 cttcatgtac ccgccggggg catcggtgtt gctcgcgccg atcaccgtgt tcggcaagct
     1561 gccgatgatg atcgtgctcg tcgcgatcaa caccttcgcc tgggtcgtct gcatcctggc
     1621 gccgatctac ctcgtcgccg gcacaacccg ccagcagccg ctgattcttt acgtcctgcc
     1681 gacggcggtg tgcatcgtct acatctggtc gagctacctg ctgggcaacc cgccgattgt
     1741 tctagccgcg tgcgtgctgg gcatgttcat ctgcctgcgc aaacgcatcg actggtccgc
     1801 cgggctgctg ctggcgctgg ccgcggggtt taaggctttt ccaatcctgg cggtgccgta
     1861 tttgatgtgg cggcgacatt ggcgcgcgct gggttacacc gtcgcctttc tgttcatcct
     1921 gctgctggtc ctgcccgcgc tcttccgcgg tgttcacggc gcggcggccg acttaaatct
     1981 gtggacccac gacacgctcg gcagctacaa cggcgagcaa atcggacagc gcaaacagcg
     2041 cagctatttg tggaagaacg gatcgatttt cgccgaagcc catcgcctgc ttcgatcggg
     2101 cgtggtcgcg gattatgacg acaattcgcc ggaagagccg gcgctgaccg tgaacgtggc
     2161 atcgttgtca ttcacgcagg tcaatctcgt catcctgatc gcgaccgcca tcctatgcgg
     2221 gtcatatctt ctcgtgatgc cgcgcggcgc caatcgcacg ccgtggagcg atgcgatcga
     2281 aggggcgatg ctgatgatcc tgatcatcac ctacacgccg ttgtcgttca cgtacaacaa
     2341 ctcctggctg atgctgccga tcgtggtggt attgtatttc atctgttgca aggcgcgctc
     2401 ggcgacggag aaacgactgg cggtcgcatg gctcgccatc gccctgctgc tgctgatctt
     2461 caccgccgga aagatcccgt tcttccgcta catgcgctcg ctcggcaaca ccttctggtg
     2521 ctgcatgctg ctgtacgcgg agctgatctg gatcatgttg caggcacgcc gtgagagcaa
     2581 cgtcctacag cccgagcaat ccgtttagat cggccagcgc cgcggcggtt tctcgcatca
     2641 acaccgggtc cgccaaatcc gccgccgata gcatctcgcg atagtgccgc ttcaccgacg
     2701 cgaccagctt cgcgtatagc gcctcgtcca gcatcacccc cgcatggacc gctcgcaact
     2761 gctcatccgt cagcacgacg cgcaaccgca gacacgcggg cccgccgccg ttgaacatgc
     2821 tttgccgcac gtcgagataa tgcaccgcgc ggattggatt atcctccgcc agcacccgct
     2881 gcaaaaacgc ctgcacccgc ggatgatcac ggcattcgac cggcgcgatc agcgccatcg
     2941 cgccatccgg tagcgtcacc agttggctgt tgaacacgta actctgcacc gcgtccgcca
     3001 gcgacaactc cgcctcggcc gccgcaatga tcgtcggctc ccacgacagt gactggcgaa
     3061 tctccgccag cgcattgccc gcgaatgcta aagcatgaca cagcagcacg cgctcgttgc
     3121 tcaccgcgac gacatcgttg tgaaacg
//
LOCUS       DATIMH010000052         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1454736, whole genome shotgun sequence.
ACCESSION   DATIMH010000052 DATIMH010000000
VERSION     DATIMH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454736"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1863
                     /locus_tag="VLI90_00890"
     CDS             <1..1863
                     /locus_tag="VLI90_00890"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HSV12784.1"
                     /translation="EQPPATPYQPLQTDQPPPSTPPPGKAPVSDYVDIAPWDNSNSQY
                     GVLGVWAGAETGVEVPTKYWKAVQQHWQSCELKTGEWFYAWINPPQTPTPTLQMTCAG
                     IASLLITHDYLDAPALGNRVGRPPYSPNLSTGLSWLESGDNVINVITENMFYVGYNLY
                     GIERCALASGYKYFGTHDWFAELCGQVLPNQYPNGAWGRGPDGEAALIDTSYIVLFLS
                     RGRHPVIMNKLRFDPFWDNRPRDLANLAKFASHELERPLNWQVVNLQRDWSDWMDAPI
                     LYIASHQAPKFTDEDYDKLRSFAEAGGMIFTHADSAAPAFNNWVDATTKRLFPQYALR
                     DVPGDHELFSVNYKLKSPTHLKMVTNGTRLLWVHSPVDISPAWQERGFKTSLSTFQLG
                     VNLFVWAAGRGDFRNRLDSPYIPQPSGDPQQTITVARLKYGDGPAWNPEPYAWTRFGR
                     IVQTDTGTAVKTVDVDAKDLSAIGDYKIAALTGNAATDFTDDQIKAIASFVESGGTLL
                     IDACGGSTDFATCIEQKLLPEAFPNTPMKLLPADDPIAKTAGAPAIRQYTVAKLGSAD
                     APPVKQISFGKGRVIYSPLDLTTGLLGTNSWGIFGYTPTYSVALLKSIASAAQK"
     gene            2304..2522
                     /locus_tag="VLI90_00895"
     CDS             2304..2522
                     /locus_tag="VLI90_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12785.1"
                     /translation="MRALKFLARQAKLFSRRIEADDAATFWTSRHREVLQQIPAAANV
                     GGLTTWAGPDQTSNRAAKEEQRDERQDG"
BASE COUNT          538 a          917 c          814 g          467 t
ORIGIN      
        1 gagcaaccgc cagccacgcc gtatcaaccg ctgcaaaccg atcaaccccc gccgtcaaca
       61 ccgccgccgg gcaaggcgcc ggtttccgat tacgtcgaca tcgctccgtg ggacaactcc
      121 aactcccaat acggcgtgct cggcgtttgg gccggtgctg aaaccggcgt cgaggtgccg
      181 accaaatact ggaaagccgt ccagcagcac tggcaaagct gcgagttgaa aaccggcgaa
      241 tggttttacg cgtggatcaa tcccccgcag acgccgacgc cgacgttgca gatgacctgc
      301 gccggcatcg cgtcgctgct gatcacgcac gactatctcg acgccccggc gctgggcaac
      361 cgcgtcggcc ggccgccgta ttcgccgaat ctttccaccg gtttgagctg gctcgaaagc
      421 ggtgacaacg tgatcaacgt catcaccgaa aacatgttct acgtcggcta caacctgtac
      481 ggcatcgagc gctgcgcgct ggccagcggg tacaaatatt tcggcaccca cgattggttc
      541 gccgagttgt gcgggcaggt gctgccgaac cagtacccca acggcgcctg gggccgcggg
      601 cccgacggtg aggcggcgct gatcgacacg tcctacattg tcctgttcct gtcgcgcggc
      661 cggcacccgg tcatcatgaa caagctgcgg ttcgacccgt tctgggacaa tcgcccgcgc
      721 gacctggcga accttgcgaa attcgcctcg cacgaattgg agcgcccgct caactggcag
      781 gtcgtcaacc tgcagcgcga ctggtccgac tggatggacg cgcccatcct gtacatcgcc
      841 agccatcagg cgcccaagtt caccgatgag gattacgaca agctccgcag cttcgccgag
      901 gccggaggca tgatcttcac ccacgccgac agcgccgcgc cggcattcaa caattgggtg
      961 gacgcgacga cgaaacggct cttcccgcaa tacgcgttgc gcgacgtgcc gggggatcac
     1021 gaactgttca gcgtcaacta caaactgaaa agtccaacgc acctgaagat ggtcaccaac
     1081 ggcacgcgcc tgctgtgggt gcattcgccg gtggatatct cccccgcctg gcaggagcgc
     1141 gggttcaaga catccctgtc cacattccag ctcggcgtga acctgttcgt ctgggccgcc
     1201 ggccgcggtg atttccgcaa ccggctcgac tcgccgtaca tcccacagcc cagcggcgat
     1261 ccgcagcaga cgatcacggt ggcgagactg aaatacggcg acggtcccgc gtggaacccc
     1321 gaaccgtacg catggacgcg cttcggccgg atcgtgcaga ccgacaccgg caccgcggtc
     1381 aagacggtgg atgtcgatgc gaaagatttg agcgcaatcg gcgactataa aatcgccgcg
     1441 ctgacgggca acgccgccac cgacttcacc gacgaccaaa tcaaagccat cgccagcttc
     1501 gtcgaatccg gcggcacgct gctcatcgac gcctgcggcg gatcgaccga tttcgccacc
     1561 tgcatcgaac aaaaacttct tcccgaagcc ttccccaaca cgccgatgaa gctcctcccc
     1621 gccgacgacc cgatcgccaa gaccgccggc gccccggcga tccgccaata caccgtcgcc
     1681 aagctcggct ccgccgatgc cccgccggtg aagcaaatca gcttcggcaa aggccgcgtg
     1741 atctacagcc cgctggacct gacgaccgga ttgctgggca ccaacagctg gggcatcttc
     1801 ggctacacac cgacgtattc agtcgcgctg ctgaagagca tcgcctcagc cgcgcagaaa
     1861 taagatggcg ggctttcgga caacggcgct atcaaccata tcaactgcgc gcgctctctc
     1921 ttgatagccg caggcgacag ccagcgggtg ctttcgaaag cggcgccggc cgacgtcctt
     1981 cccatgcgcc atcctttggt tcgactgcct ggcaatcacc tctggttttg gacttgcgtc
     2041 acgatgggca aggccgagaa gagcgcgcaa ggtgccgcgg gggcttgcgt attacctgca
     2101 tcgagagatc aatccccctt cgcccggatt gctgcagatc ggcgggcgcc ggatttgatg
     2161 tccttccgga gcggagtggc cgaacctggt cgctgcggcc tggcgtgaag ccacgcccac
     2221 cgcggcggcc ggttcggctt gtagagctgc gcgccaatct cgtttcaggt ttttgaagtt
     2281 cattggcgct ggcttcgccg agcttgcgcg cgctcaagtt cctcgcgcga caggccaagt
     2341 tgttcagccg tcgcatcgaa gccgacgacg cggccacatt ctggacatcg cggcaccgtg
     2401 aggttctcca gcaaataccc gcagcggcaa acgtgggtgg gctgaccact tgggccgggc
     2461 ctgatcagac gtcgaaccga gcggcgaagg aagaacagcg cgacgagcgc caggatggtt
     2521 agggagatgg aaagttgcgg gatcaccgcg ggtgggtcga gctaatgcag ccgcttgcgg
     2581 cttcgcaaat cgcggcacct ggtctgcgaa gccgcaagcg gctgaaacgc gcgtctactt
     2641 caaccgcgta tcactgttgg tgatcccgcg caaggccagc ttcagagcat ccacgttgct
     2701 cgccagccgg ctggcttcgt tgatgtcgat cacctg
//
LOCUS       DATIMH010000053         4252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4631700, whole genome shotgun sequence.
ACCESSION   DATIMH010000053 DATIMH010000000
VERSION     DATIMH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4631700"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1776
                     /gene="pgm"
                     /locus_tag="VLI90_00900"
     CDS             133..1776
                     /gene="pgm"
                     /locus_tag="VLI90_00900"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HSV12786.1"
                     /translation="MAIHPLAGRHAPASMLIDVAQLEREYYERKPDPADPRQLVSFGT
                     SGHRGTTSDNTFTESHILAITQAIAEHRKSKGIDGPLYMGKDTHALSTVAQRTALEVL
                     AGNGVEVNVARQDGVTPTPVISWWILTHNHGRKSGLSDGIVITPSHNPPSDGGFKYNP
                     TNGGPADTDVTSWIQNRANELLKSGNRDVRRTPYESALKAATTHHHDFISPYVDDLDS
                     VIDLAAIQNARVRIGVDPLGGASLPYWEPIRDAYHLDLTVVNKRIDPKFSFMPVDHDG
                     KIRMDCSSPYAMADLVQLKDQYRVAFGNDPDSDRHGIVTPSAGLMNPNHYLAVAINYL
                     LTHRPHWPGRAAVGKTLVSSSMIDHVVHRLGRPLAEVPVGFKWFADALFHGTYCFGGE
                     ESAGASFLKMDGSVWTTDKDGIILNLLAAEITARTGKDPGQHYNELTLEFGTPYYTRI
                     DAPATPAQKAAFKNLTPEAVTATELAGEKILAKLTKAPGNGASIGGLKVVTPSGWFAA
                     RPSGTENIYKIYAESFKSEQQLSAIVEQAQSIVNKSLGG"
     gene            complement(1901..>4252)
                     /gene="carB"
                     /locus_tag="VLI90_00905"
     CDS             complement(1901..>4252)
                     /gene="carB"
                     /locus_tag="VLI90_00905"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSV12787.1"
                     /translation="DETLTTQMKSVGEAMAIGRTFKEALQKGIRSMEVKRFGFGLDKY
                     DKWLNAEKGVTNAAHGSDSDKTTQGESLDQEWPIPADKLRRKLAVPSQGRLYYLRYAL
                     KMGWTIDQVYQLTKIDRWFLAQMKELVEFESRIMKPNAVPTFLREAKELGYSDIQIAA
                     ARGTDAINVRVERMPSHLNVQPVYKLVDTCAAEFEAATPYYYSTYETPYTTIGKDAKP
                     QAATEDEIRLTDKPKVIIIGGGPNRIGQGIEFDYCCVQAAFAMRELGIESVMINSNPE
                     TVSTDYDTSDLLFFEPLTHEDVLNICQRLNGGPFATVSGGAQHRGLVQGVIVQFGGQT
                     PLNLARGLMEAGVPILGTSVDSLDAAGDREQFRTLLQKLGLKQPANGIARSVAEAREI
                     AKRIGYPVLVRPSFVLGGRAMEIVSDEDQLNYYMANAVEASTIADAPILVDKFLDAAT
                     EVDVDCIADFDPVPPLAASQAQASQIREAASGGQAFVIGVMEHIEEAGIHSGDSACSL
                     PPYSLSKEIIAELKRQTKELAKALRVRGLMNVQYAIKDGEIYVIEVNPRASRTVPFVS
                     KAQGQPFAKIAAKVMAGKTLAELGMVEPTPPKHISVKEVVFPFTKFPGVDVILGPEMR
                     STGEVMGIDVNFPMAFAKSQIAAGMMLPTKGNAFLSVRDEDQKALIPIARMLTDAGFE
                     LYTTSGTHAALAAADIQSTRLNKLAEGRPNIRDFIKNSQVQLIINTPTKKGPATDEGK
                     IRAMAVLNKVPMVTTMTGALAVAKAIQELQKGDWGVRPLQSYH"
BASE COUNT          781 a         1329 c         1311 g          831 t
ORIGIN      
        1 tgctcgacgc gcgggaagaa tccgggtcgt cggagtacct ccgaacccgg cgtggggaaa
       61 ctcaagccgc gcgaatcgct gatgtacaca atcgccgccc aacggtaggt tatccgcgaa
      121 gggaacaacc gcatggccat ccacccgctc gccggccggc acgcgccggc ttcgatgctg
      181 atcgacgtcg cccagctgga acgcgaatat tacgaacgca agcccgaccc ggccgacccg
      241 cggcagctgg tcagcttcgg caccagcggc catcgcggga cgaccagcga caacacgttc
      301 accgagtcgc acatcctcgc catcacgcag gcgatcgccg agcatcgcaa gtccaaaggc
      361 atcgacggcc cgctgtacat gggcaaggac acgcacgcgc tgtccaccgt cgcgcagcgc
      421 acggcgttgg aagttttggc cggcaacggt gtcgaggtga acgtcgcgcg acaggacggc
      481 gtgacgccga cgccggtcat ctcgtggtgg atcctgacgc acaatcatgg ccgaaagagc
      541 gggttgagtg acggcatcgt catcacgccg tcgcacaacc cgccgagcga tggcgggttc
      601 aagtacaacc cgaccaacgg cgggccagcc gacaccgatg ttacttcgtg gattcagaac
      661 cgcgccaacg agctactgaa atccggcaac cgcgacgtgc ggcggacgcc atacgaaagc
      721 gcgctgaagg cggcgacgac gcatcatcac gatttcatct cgccctacgt cgacgacctg
      781 gacagcgtga tcgacctggc ggcgattcag aacgcccgcg tgcgcatcgg cgtcgatccg
      841 ctgggcggcg cttcattgcc atactgggag ccgattcgcg atgcatatca cctcgatctg
      901 accgtggtga acaagcggat cgacccgaag ttcagcttca tgcccgtcga tcacgacggc
      961 aagattcgta tggattgttc gagtccctac gcgatggcgg acctggtgca gctgaaggac
     1021 cagtatcgcg tcgcgttcgg caacgatccg gattcggatc gccacggcat cgtaacgccg
     1081 agcgcgggct tgatgaaccc taatcactat ctcgcggttg cgatcaacta cctgctgacg
     1141 caccgcccgc attggccggg gcgggcggcc gttggcaaga cgctggtcag cagcagcatg
     1201 atcgatcacg tcgtgcatcg gctgggccga ccgctggcgg aagtgccggt cggtttcaag
     1261 tggttcgccg atgcgctgtt tcacggcacg tactgtttcg gcggcgaaga gagcgcgggc
     1321 gcgagcttcc tgaagatgga cggcagcgtg tggacgaccg acaaggacgg catcatcttg
     1381 aacctgctgg ccgcggagat cacagcgcgc acgggcaagg atccggggca gcattacaac
     1441 gagctgacgc tggagttcgg cacgccgtac tacacgcgca tcgacgcccc tgcgacgccg
     1501 gcgcagaagg cggcgtttaa gaatctcacg cccgaagcgg tcaccgcgac cgaactggcc
     1561 ggcgagaaga tcctcgcgaa gctgacgaag gcgccgggca acggcgcgtc gatcggcgga
     1621 ctgaaggtcg tcacgcccag cggttggttc gcggcgcgac ccagcggcac cgagaacatc
     1681 tacaagattt acgcggagag ttttaagagc gagcagcaac tgtcggcgat cgtcgagcag
     1741 gcgcagagca ttgtgaataa gtcactggga ggatgatcgt gaatgacgaa atcggaatga
     1801 cgaaacatgc gagatcgcat gggtttgggc attcgtgctt cgtcattcgg atttcattcg
     1861 gcgattcgaa tttcgtcatt cgaatttcag ctcgccgggt tcagtgatac gactgcaacg
     1921 gccgcacgcc ccagtcgccc ttttgcagtt cttgaatcgc cttggccacc gcgagggcgc
     1981 cggtcatcgt cgtcaccatc ggcaccttgt tcagcaccgc catcgcgcgg atcttgcctt
     2041 cgtccgtcgc ggggcccttc ttggtcggcg tgttgatgat cagctgcacc tgcgagttct
     2101 tgatgaagtc gcgaatattc ggccggcctt cggcgagctt gttcaatcgc gtcgattgaa
     2161 tgtccgccgc ggcgagcgcc gcgtgtgtgc cgcttgtcgt gtacagctcg aaaccagcgt
     2221 cggtcagcat ccgcgcgatc gggatcaggg ctttttgatc ttcatcgcgc acgctcagaa
     2281 aagcgttgcc cttggtcggc agcatcatgc ccgcggcgat ctggctcttg gcgaacgcca
     2341 tcgggaagtt cacgtcgatg cccatcactt cgccggtcga acgcatctcc gggccgagga
     2401 tcacgtcaac gccggggaac ttggtgaacg ggaaaacgac ttccttcacg ctgatgtgct
     2461 tgggcggcgt cggttcgacc atgcccagct ccgccagcgt cttgccggcc atcaccttgg
     2521 cggcgatctt cgcgaagggc tggccttggg ccttggagac aaacggcacc gtccggctgg
     2581 cgcgggggtt cacttcaatc acgtaaatct cgccgtcctt gatggcgtac tgcacattca
     2641 tcagcccgcg gacgcgcagc gctttggcca gttccttcgt ctgccgtttc agctccgcga
     2701 tgatctcctt ggacaacgaa tacggcggga gcgagcaggc gctgtcgccg ctgtggatgc
     2761 ccgcttcttc gatgtgttcc atcacgccga tgacgaaggc ttgaccgccg cttgcggctt
     2821 cgcggatctg ggacgcctgg gcttgcgaag ccgcaagcgg cggaacggga tcgaaatccg
     2881 cgatgcagtc cacgtccact tccgtcgccg cgtcgaggaa cttgtcgacc aggatcggcg
     2941 cgtccgcgat cgtgctcgct tctactgcat tcgccatgta gtaattcagc tgatcttcgt
     3001 cgctgacgat ttccatcgcc cggccgccca gcacgaagct gggacggacc agcacgggat
     3061 agccgatgcg cttggcgatc tcgcgcgcct cggccaccga tcgcgcgatg ccgttggcgg
     3121 gttgtttcag tcccagcttc tgcagcaacg tgcggaactg ctcacggtcc ccggcggcgt
     3181 cgagcgaatc gacgctggtg ccgaggatgg gaacgccggc ttccatcagg ccgcgggcca
     3241 ggttcagcgg agtttgtccg ccgaattgga caatgacgcc ttgaactaac ccgcggtgct
     3301 gcgcaccgcc gctaactgtc gcgaaggggc cgccgttcag gcgctggcaa atgttcagca
     3361 cgtcctcgtg cgttaggggc tcgaagaaca gcaggtcgct ggtgtcgtaa tcggtgctga
     3421 cggtttcggg gttcgagttg atcatgaccg actcgatgcc gagctcgcgc atcgcgaacg
     3481 ccgcctgcac gcagcagtag tcgaactcga ttccctggcc gatgcgattc ggaccgccgc
     3541 cgatgatgat caccttgggt ttgtcagtga ggcggatttc gtcttcggta gccgcttgcg
     3601 gcttagcgtc ttttccgatc gtggtgtatg gcgtttcgta tgtggaatag tagtaaggcg
     3661 tcgccgcctc gaactccgcg gcgcatgtat cgacgagctt gtagacaggc tgcacattca
     3721 aatgcgatgg catgcgttcg actcggacgt taatcgcgtc ggtacctcgt gcggcagcaa
     3781 tctgtatatc actgtagccc aactcctttg cctctctcag aaatgtggga accgcattgg
     3841 gcttcatgat gcggctttca aactccacca actccttcat ctgcgccaag aaccaccgat
     3901 cgatcttcgt cagctgatac acctgatcga tcgtccatcc catcttcagc gcgtagcgca
     3961 ggtaatacag ccgtccctgg ctcggcaccg cgagcttgcg gcggagcttg tcggcgggga
     4021 tcggccattc ctggtcgagc gattcgccct gcgtggtctt gtcggagtcc gacccatgcg
     4081 ccgcattcgt cacgcccttc tcggcgttca gccacttgtc gtacttatcc aggccgaagc
     4141 caaagcgctt cacctccatc gagcggatgc ccttctggag ggcttccttg aacgtccgcc
     4201 cgatggccat cgcttcgccg acggacttca tctgcgtcgt cagcgtctcg tc
//
LOCUS       DATIMH010000054         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3187862, whole genome shotgun sequence.
ACCESSION   DATIMH010000054 DATIMH010000000
VERSION     DATIMH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3187862"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VLI90_00910"
     CDS             <1..457
                     /locus_tag="VLI90_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HSV12788.1"
                     /translation="FFINSPGGSVSAGFGIVDTMKYLRCDVATTCIGMAASMGAILLA
                     AGTKGKRACLANSQVMLHQPLIGGVLQGPATDLGIEAQHMLRLRDRMYKLMANWTGKT
                     PEQIHRDFDRNKWLFAEEAVEYGAADRVLDRIPEVAMRPRPRSEDDEKE"
     gene            555..1145
                     /locus_tag="VLI90_00915"
     CDS             555..1145
                     /locus_tag="VLI90_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12789.1"
                     /translation="MLLLSGCGAPSAANIELRKDNQKLQDQVTHLQRQHDADVATIQS
                     YERSRPTVPTLPQERLDKLFTTHGIAFQRITGGDDWDPNQPGDDGLKIGIVPTDEQGE
                     ELKAAGSFKVEAFDLGEPEKPLIGTWTFSPEQVRSMFYDHLTLYTYVLRCPWQTRPTH
                     GDLMVKVTFDDELTGREFTATREVHANVPATTTTQP"
     gene            complement(1264..1410)
                     /locus_tag="VLI90_00920"
     CDS             complement(1264..1410)
                     /locus_tag="VLI90_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12790.1"
                     /translation="MPACREPAPPPVTPSPPPPVPTAAANSTQQASTITSPTHDAPPR
                     QPAN"
     gene            1548..2261
                     /locus_tag="VLI90_00925"
     CDS             1548..2261
                     /locus_tag="VLI90_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSV12791.1"
                     /translation="MSKDRPINRRSFFRQGLSELLRPLAQAAEPLEEVIRQISSIDQE
                     VEAAARAQAARAAKGQTPPAPVVRAAPGVWHRPPGALPEQQFRDTCSKCGECVNVCPA
                     QCIKIDPNGAKGAGVPYIDVDSMPCVLCDGLLCMHNCPTGALVPTPIGDIDMGTAVWH
                     EETCLRTRGEDCTICVDQCPVGEMALVLRDGRIEVLEAGCTGCGVCQHRCPTSPKSIT
                     VIPRMTGGESGGAVARSRR"
     gene            2542..3102
                     /locus_tag="VLI90_00930"
     CDS             2542..3102
                     /locus_tag="VLI90_00930"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSV12792.1"
                     /translation="MLDTQQLPDNVLTLQLKKVVNWARRSSLWPMPFATACCGIELMA
                     TASSRYDLARFGAEAMRFSPRQSDLMIVAGRVTIKMMPVLQHIYQQMTEPKWVISMGA
                     CASTGGVFDAYATIQGIDQFMPVDVYVPGCPPRPETLIEGIMAIQRIIDEDGLPPAGK
                     RRPLQLAIEPSYAPSPQPVPLTIGGR"
     gene            3240..>3586
                     /locus_tag="VLI90_00935"
     CDS             3240..>3586
                     /locus_tag="VLI90_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12793.1"
                     /translation="MADDPNELIPPDVLALIAGLDSVAQLEILLLLHREPSRQWTAEQ
                     VAGELRINPAAAAEQMNVLCDRQLLACDGGRSKQYHDAAHTPQVAAAIDQLTRLYNER
                     RVTVITLVFTKPS"
BASE COUNT          647 a         1109 c         1203 g          627 t
ORIGIN      
        1 gttcttcatc aactcgcccg gcggcagcgt gtcggcgggg tttggcatcg ttgacacgat
       61 gaagtacctg cgctgcgacg tggccaccac ctgcatcggc atggccgcca gcatgggggc
      121 gatcctgctg gccgccggca ccaagggcaa gcgggcctgc ctggcgaata gccaggtcat
      181 gctccaccag ccgctcatcg gcggcgtgtt gcaaggtccg gccacggact tgggcatcga
      241 agcccagcac atgctccggc tccgggaccg catgtacaag ctgatggcga actggaccgg
      301 caagacgccg gagcagatcc accgcgactt cgatcgcaac aagtggctct tcgccgagga
      361 agccgtcgaa tacggcgccg ccgaccgggt gctcgatcgc atccccgaag tcgcgatgcg
      421 cccgcggccg cgcagcgaag atgatgagaa ggaatgagat cctccaaatg gagaaggata
      481 aatgcagaat gcagaatgga cgggctaaag ccttcttcca ttctgcattc tgcattctgc
      541 attctgcatt tgttttgttg ctgctgagcg gctgcggcgc ccccagtgcc gccaacatcg
      601 aactccgcaa ggacaaccag aagctgcagg accaggtcac gcacttgcag cggcagcacg
      661 atgcggacgt ggcgacgatc cagtcgtacg aacgcagccg gcccaccgtg ccgacgttgc
      721 cgcaggagcg gctggacaag ctgttcacca cgcacggcat cgcgttccag cgcatcaccg
      781 gcggtgacga ttgggatccg aaccagcccg gcgacgacgg cctgaagatc ggcatcgtcc
      841 ccacggatga gcagggcgag gaattgaaag ccgcggggtc attcaaggtc gaggccttcg
      901 acctgggcga gccggagaag ccgctgatcg gcacgtggac gttcagcccc gagcaggtcc
      961 gttcgatgtt ttacgatcac ctgacgctgt acacatacgt gctgcgttgc ccctggcaga
     1021 cgcggccgac gcatggggat ttgatggtga aggtgacgtt cgatgatgag ctgaccggcc
     1081 gggagtttac cgcgacgcgc gaggtgcacg cgaacgtgcc ggcgacgacg acaacgcagc
     1141 cgtgatgata aaccacgccg ggttcggagg tactccgacg acgcggatct cttcgcgcgc
     1201 cgcagcatcc gggtcgtcgg ggtacctccg aacccggcgt gcgggggtat gaatcatcct
     1261 cgatcaattc gccggctggc gcggcggagc atcgtgcgtg ggcgacgtga tcgtcgacgc
     1321 ctgctgcgtc gagtttgccg ccgccgttgg caccggtgga ggcggcgacg gggtgaccgg
     1381 cggtggcgcg ggttcgcggc aggcgggcaa ggttgcggct aaaatcaaaa ggcaggcgag
     1441 cgttcggtat cgagtggctt gcattcgcgt acgtttccct gaagcgagcg aggctgtagc
     1501 gttcgaagca taccgcgtgt cccggccgcg tgacaagttt tcgttgcatg tccaaggatc
     1561 gccccattaa tcgccgcagc ttcttccggc agggattgag cgagctgctg cgcccgttgg
     1621 cgcaggcggc agagccgctg gaggaagtca tccggcagat cagctcgatc gaccaggaag
     1681 tggaagcggc ggcccgggcg caggcggctc gcgccgcgaa ggggcaaact ccgccagcgc
     1741 cggtcgttcg cgcggcgccg ggcgtctggc atcgcccgcc aggggcgctg ccggagcagc
     1801 agtttcgcga cacctgcagc aagtgcggcg agtgcgtgaa cgtctgcccg gcgcagtgca
     1861 ttaagatcga tcccaacggt gccaaaggcg ctggcgtgcc gtacatcgat gtcgattcga
     1921 tgccgtgcgt gctgtgcgac ggcctgctgt gcatgcacaa ttgcccgacc ggcgcgctgg
     1981 tgccaacgcc gatcggcgac atcgacatgg gcaccgcggt atggcacgag gaaacctgcc
     2041 tgcgcacgcg cggcgaagac tgcacgatct gcgtcgatca atgcccggtg ggcgagatgg
     2101 cgctggtcct gcgcgacggc cgcatcgaag tgctagaagc cggctgcacc ggttgcggcg
     2161 tctgccagca ccgctgcccg acgagcccga agagcatcac ggtcatccca agaatgaccg
     2221 gcggcgaaag cggcggggcg gtggcgcgct cgcggcgcta gcgcgcgact ggacttgccc
     2281 tctcgcccgg tactccggga gaggggtgag ggtgatttct gatgcagaat gtccaatgtc
     2341 caagcaccaa atcccattga agtcacaatg accaagtctc gcgtgggcat tggtacttgg
     2401 gccttcattg ggatgtggga tttgctcatt ggtcattatc cgcaaaccac cctcactcta
     2461 accctctccc ggcgtaccgg gcgaggggac cggggcgcgt gcgctggcga tcatcgcccc
     2521 gccgcctata atcccttcac catgctcgac acccagcagt tgccggacaa tgttttgacg
     2581 ctccagctca agaaggtcgt gaactgggcc cgccgcagtt cgctttggcc gatgccgttt
     2641 gccaccgctt gctgtgggat cgaattgatg gccaccgcgt cgtcgcgtta tgacttggcc
     2701 cgcttcggcg ctgaagcgat gcgcttcagc ccgcggcaga gcgatttgat gatcgtcgcc
     2761 ggccgggtga cgattaagat gatgccggtg ctgcagcaca tttatcagca gatgaccgag
     2821 ccgaagtggg tgatcagcat gggcgcgtgc gccagcacgg gcggcgtgtt cgatgcgtac
     2881 gccaccatcc aaggcatcga ccaattcatg ccggtggacg tgtacgtgcc gggttgtccg
     2941 ccgcggccgg agacgctgat cgaaggcatc atggcgatcc agcggatcat cgacgaagac
     3001 ggcctgccgc cggccggcaa gcgccggccg ctgcagctgg cgatcgaacc cagctacgcg
     3061 ccgtccccgc aaccggtgcc gctgacaatc ggcggccgtt gagcaacctc gaggcaactc
     3121 gcaaagctgg gcgaattcga gcgcccggct tttctttttt gcgcgttgcc acgcgcggac
     3181 gatgcggtta cggtcccgcc tcctgatgca tttcgtggac tttgggtgcg gttaccgccg
     3241 tggcagacga tcccaacgag ctcattccgc cggatgttct cgcgttgatc gcggggctcg
     3301 attcggttgc gcagctggag attttgctgc tgctgcatcg cgagccatcg cggcaatgga
     3361 cggccgagca ggttgcggga gagcttcgca tcaatcccgc cgccgccgcc gagcagatga
     3421 atgtgctgtg cgatcggcag ttgctggcgt gcgatggcgg gcggtcaaag cagtatcatg
     3481 acgccgccca cacgccgcag gtcgcggcgg ccatcgatca attgacccgg ctgtataacg
     3541 agcggcgcgt gacggtgatc acgctggtct tcaccaaacc gtcgga
//
LOCUS       DATIMH010000055         4310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4632059, whole genome shotgun sequence.
ACCESSION   DATIMH010000055 DATIMH010000000
VERSION     DATIMH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4632059"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..711
                     /gene="ssb"
                     /locus_tag="VLI90_00940"
     CDS             136..711
                     /gene="ssb"
                     /locus_tag="VLI90_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSV12794.1"
                     /translation="MASFNKVLLMGNLTRDPQLKYLPSQMAVVEFGLACNRRFKTAAG
                     EDREEVTFVDVSAFGKTGELINQYFTKGKPIFIEGRLKYDQWEDKNGGGKRSKLAVVA
                     ENFQFIGGRDGGGAGGGGGGGGGYESNAGGGGGGGGDEYEQRPSRPAPQGRPMPQQNR
                     PAPQQRPQPQQPPPEQPFGEEQAFKEDDIPF"
     gene            780..1313
                     /gene="rplI"
                     /locus_tag="VLI90_00945"
     CDS             780..1313
                     /gene="rplI"
                     /locus_tag="VLI90_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSV12795.1"
                     /translation="MANVKLLLKESVKNVGRVGDLVEVSPGYARNFLLPKDLAVHPTP
                     NNVKKVEERKKEIERQERERREQQAALIKQLEGVEVTLERRANEQGHLFGSVSATDIA
                     KALQAQGFNIEAADVNLPGKLDRIDTYQVQIKFADDLATDVKVWVAPDAESKAAIDAA
                     RKTAAENPPPASNFEKA"
     gene            1584..3980
                     /gene="dnaB"
                     /locus_tag="VLI90_00950"
     CDS             1584..3980
                     /gene="dnaB"
                     /locus_tag="VLI90_00950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSV12796.1"
                     /translation="MDQLIDASIAKQFDRLPPHSIEGEMCLLASMMLDKEIIGQAVQI
                     IDREAFFQADHQIIYDVLVKLYEQNRPIDAIILRDELGKIQLLEEIGGQAYLGQILNT
                     VPSAAHGEHYAKIVREKALLRQLIAASNDILRDAYAPHEQADLVLDKAEKRIFEIASK
                     KVGNSVVAMETVLHEVFEMIENRGQRGVETGFFELDDMMNGLQNGEMVIVAARPSMGK
                     AQPLDAKVLTTTGFKTMGELSLDDELASIDGAPSKVTGIYPQGERQVYRITLSDGRST
                     ECCAEHLWRVHYRNWPAPRVLSTAKIMQLLTRQRYQGRIWLESFCGEFGDDAKELPVD
                     AWLLGALLGDGSLSGSSLRFSTASDALRQKIEAVIGAERVVTHAGNYDYRIIQRESKR
                     RGVQGGLPNPIIAALRALGLWERDAASKFIPASYLKASRPTRLQLLAGLIDTDGWVEK
                     FGAIRLATCSSRLANDIVQLTRSLGGSASFYPKQTTYTYKGAKCVGQTTFVCNLQLPN
                     PFELQLLSPKVERLARKRQRRLTIAAIVPSRITQTQCIAVDHDSHLYITDDFIVTHNT
                     AFAMNIIEHVSADSLLPCAVFSLEMSKQQLAQRMLCSRGQIDAHKLRKGMLQAHEYAH
                     LANVVGELAKAPIWVDDSPGLTPLELRAKARRLKLQHDIKLIMIDYMQLMDNPGVESR
                     QQQISEISRGIKAVARELNVPVIALSQLNRASEGRDGHRPRMSDLRESGSIEQDADVI
                     ALLHREDYYRMSEPDFQPDNIAEVIIAKQRNGPTGTVKLTFMNKTTRFENLSTHADPF
                     "
BASE COUNT          939 a         1328 c         1293 g          750 t
ORIGIN      
        1 ggccgcagaa gctggcgctg ccaaagattg agcgtgactc cgtcctcacc gtctcgtatg
       61 gtacggggcg aacattaccc tacctcacgg acggggtgtg catggtgaac caccgctcga
      121 accccggagg tatcgatggc cagcttcaat aaagtcctgc tgatgggcaa tctcacgcgc
      181 gacccgcagc tgaaatatct gccgagccag atggcggtgg tcgagttcgg cctcgcctgc
      241 aatcgccggt tcaaaacggc cgcgggcgaa gatcgcgagg aagtcacatt cgtcgatgtg
      301 tcggccttcg gaaagactgg agagttaatc aaccagtact tcacgaaggg caagccgatc
      361 ttcatcgaag ggcgactgaa gtacgatcag tgggaagaca agaacggcgg cgggaagcgc
      421 agcaagctgg ccgtggtggc cgagaatttc cagttcatcg gcggccgcga cggtggcggc
      481 gccggtggtg gtggtggcgg aggcggtgga tacgaatcca acgccggtgg cggcggcggc
      541 ggcggtggtg acgaatacga gcagcgtcca tcgcgtcccg ccccgcaggg tcggccgatg
      601 ccgcaacaga atcgcccggc gccgcagcag cgcccgcagc cgcagcaacc gccgccggaa
      661 cagccgtttg gtgaagagca ggcctttaag gaagacgata ttccgttcta gtagcatggg
      721 cgtctcgccc atgccgagca gttcgacacg ggcgagacgc ccgcgctacg aggttaaaca
      781 tggcaaacgt gaagttactt ctgaaagaat cggttaagaa cgtcggtcgc gtcggcgatc
      841 tcgtcgaggt ctcgcccggc tacgcccgca actttctcct gcccaaggat ctggccgtcc
      901 acccgacgcc gaacaacgtc aagaaggtgg aagagcgcaa gaaggagatc gagcgccagg
      961 agcgcgagcg ccgcgagcag caggctgccc tcatcaagca gttggaaggc gtcgaggtca
     1021 ccctcgaacg ccgagccaac gagcagggtc acctgttcgg ctccgtcagc gcgaccgaca
     1081 tcgccaaggc cttgcaggcg cagggcttca acattgaagc cgccgacgtc aacctgccgg
     1141 gcaagctcga tcgcatcgac acgtaccagg tgcagatcaa gttcgctgac gatctggcga
     1201 cggacgtgaa ggtgtgggtt gcgccagacg ccgagagtaa ggccgccatc gacgccgccc
     1261 gaaaaaccgc cgcggagaac cccccgccgg cgagcaattt cgagaaggct tgatatcgcg
     1321 cacgggcttt caacagctcg ctgtgcagaa gtcctgaata acctgtccgc gccgtccctt
     1381 ctgaccggcg cggacaggtt cttcgcttat atagagcacc cgcgcggcgt ggtcgcttca
     1441 gcgaccaagt cccccgccca aaacccgaaa atcccttgaa cccccaccag aacgtgttag
     1501 cttcacgttc gggtgaacga ggatgaagga agaagcgttt gtgcgcgccc gccatgcgac
     1561 gcgccaggga aggaaatttc gcaatggatc agctcatcga cgcttccatc gccaagcaat
     1621 tcgatcgcct gccgccgcat tcgatcgaag gtgaaatgtg cttgctcgcg tcaatgatgc
     1681 tcgacaagga gatcatcggg caggccgtcc agattatcga tcgcgaagca ttcttccagg
     1741 cggatcatca aatcatctac gacgtgctgg tgaagctgta cgagcagaac cgcccgattg
     1801 atgcgatcat ccttcgcgac gagctgggca agatccagct gctggaagag atcggcggcc
     1861 aagcttacct cggccagatc ctcaacaccg tccccagcgc cgcgcacggc gaacattacg
     1921 cgaagatcgt tcgcgaaaag gcgctgctcc gccagctgat cgctgccagc aacgacatcc
     1981 tgcgcgatgc gtacgccccg cacgaacagg ccgatctcgt gctcgacaaa gcggagaagc
     2041 gcatcttcga aatcgccagc aaaaaagtcg gcaactccgt cgtcgcgatg gaaaccgtgc
     2101 tgcacgaggt tttcgagatg atcgaaaacc gcggccaacg cggtgtcgag accggcttct
     2161 tcgagctcga cgacatgatg aacggcctgc aaaacggcga aatggtcatc gtcgcggcgc
     2221 ggccttcgat gggaaaagca cagccgctgg atgcgaaggt gctcaccacc accggattca
     2281 agacgatggg cgaactgagt ctcgatgatg agctcgcgtc gattgacggc gcaccatcca
     2341 aagtcaccgg catctatccc caaggcgagc ggcaggtcta tcgcatcacc ctgagtgacg
     2401 gccgatctac cgaatgctgc gctgaacatc tgtggcgcgt acactaccga aactggccgg
     2461 cccctcgcgt gttatccacc gccaagatca tgcaactcct gacgcgacag cgataccaag
     2521 gccggatatg gcttgaatcc ttctgcggcg agttcggaga tgatgccaaa gaattgcccg
     2581 tcgatgcttg gctgctgggc gccctgctcg gagacggatc gctgtcaggt tcctcgctga
     2641 gattcagcac ggcaagcgat gcgctgcggc aaaaaatcga ggcggtgatt ggcgctgaga
     2701 gggtggtcac ccatgctgga aactatgact atcgcatcat ccagcgggaa tcgaagagac
     2761 gcggcgtgca aggcggtctg cccaatccca tcatcgccgc cctccgcgcc ttaggccttt
     2821 gggaacgcga cgccgccagt aaattcatcc cggcgtcgta cctgaaggcc tcgaggccga
     2881 cgcgtctcca gctcctcgcc ggattgatcg acaccgatgg ctgggtcgaa aagttcggcg
     2941 ccattcggct ggccacctgc agctcacgcc tggcgaatga tatcgtgcaa ctgacccgct
     3001 cgctcggcgg ttcggccagc ttctatccaa agcaaaccac ctacacttac aaaggcgcca
     3061 agtgcgttgg gcagacgacc ttcgtctgca atctgcagct gcccaaccca tttgaactgc
     3121 aattgctctc gcccaaggta gagcgcttgg cacgaaagcg ccagcgtcgt cttaccatcg
     3181 ctgctatcgt tcccagccgg atcacgcaga cgcaatgcat tgcagtagat cacgacagcc
     3241 atctgtatat caccgacgac ttcatcgtga ctcacaacac cgcgttcgcc atgaacatca
     3301 ttgagcacgt gtcggctgac agcttgctgc cgtgcgcggt gttctctctc gaaatgagca
     3361 agcagcagtt ggcccagcga atgttgtgca gccgcgggca gatcgatgcg cacaagcttc
     3421 gcaaggggat gctgcaagcg cacgagtacg cgcatttggc gaacgtcgtg ggcgagttgg
     3481 cgaaggcgcc gatttgggtt gatgactcgc cgggactgac gccgctggaa ctgcgggcga
     3541 aggcgcgtcg tcttaagctg cagcacgaca tcaagctgat catgatcgac tacatgcagc
     3601 tgatggacaa cccgggcgtg gaaagccggc agcagcagat cagcgaaatc agccgcggga
     3661 tcaaggccgt ggcgcgcgag ctgaacgtgc cggtgatcgc gctgtcgcag ttgaatcgcg
     3721 ccagcgaagg ccgtgacggc caccgcccgc gcatgagcga cctgcgcgag tccggctcga
     3781 tcgagcagga cgccgacgtg atcgccctgc tgcaccgcga ggattactac cgcatgagcg
     3841 agccggattt tcagccggac aacatcgcgg aggtgatcat cgccaagcag cgcaacgggc
     3901 ccacgggcac tgtgaagctg acgttcatga ataagacgac gcggttcgag aatctttcaa
     3961 cgcacgcgga tcctttctga tcgccagaca gatacgcgcg accattccca gtagccccga
     4021 aggggcacgc gttttctttt cgaaggagaa acacgtgccc cttcggggct accgctacgc
     4081 gtgggaggac attccccgca acttcttctc ggccgattca ttctaaggta ggtcttcttc
     4141 tccaacgcca aaggccgcca tgccataccc cctcccccgc cgccgggatt tgcctgcgtt
     4201 taccctgatc gaactgatgg tggtgatcat gatcatcgcc atattaattg cgctcctgct
     4261 accggcgctg agccgcgccc gccgctccgc gatgacgaag ctggcgaacg
//
LOCUS       DATIMH010000056         4113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1166914, whole genome shotgun sequence.
ACCESSION   DATIMH010000056 DATIMH010000000
VERSION     DATIMH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166914"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VLI90_00955"
     CDS             <1..1096
                     /locus_tag="VLI90_00955"
                     /inference="COORDINATES: protein motif:HMM:NF045341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12797.1"
                     /translation="ARGWVVHHLTDVYLVTTPSDGPPGVWPMGAAWMCQHLWEHYAFT
                     GDKQFLAKQGYPLMKGAARFILDYMIPAPAGTPVAGKLVTCPSYSPENKFIDANGKQA
                     FFSYASSMDLEIIHDLLTHCVAAAKVLGSDDDFRAECEQALANLAPLRIAPDGRLQEW
                     IEDYKEQDPHHRHTSHLFAVYPGDQITLAGTPDLAAAARKSLEARTDEHATEWSFAWR
                     CALWARFHDGARAYGQLERLVSKDLYPNLFNKYPPFQIDGNLGATAALAEMLLQSQGG
                     DIQLLPALPKEWPSGHVKGLCARGGFEVEMTWKDGTLQAATITSKIGGPLRVRGNTPV
                     VAKSTGAETAKDGWITLATEAGKTYTFARE"
     gene            complement(1231..1761)
                     /locus_tag="VLI90_00960"
     CDS             complement(1231..1761)
                     /locus_tag="VLI90_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12798.1"
                     /translation="MPHFRKSSYTSNEFYAGHHRFEHWYRDNTVYFLTARCRDRFHAF
                     ASEPAKEIFWDRFDHYTAEFGFVPWATTLVDNHYHSLGYMKRGTDLGKMMQRIHGSVA
                     KLVNDLLPQRHLPFWRTAGNQDYFDGCIRDVLQAERALIGTRCCKPNDMAWSRIGASI
                     ATRISTSRSSAPSCEL"
     gene            complement(1870..3288)
                     /locus_tag="VLI90_00965"
     CDS             complement(1870..3288)
                     /locus_tag="VLI90_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12799.1"
                     /translation="MQNAARFWSILALVGCLISPHLARGASAAAPVTVTIDSANPGAI
                     IPDDFTGFSFEIQTLLPDKDGKRMFTADNKPLIALFKQLGIKSLRVGGNTADNRAVAV
                     PDEKDADSLIAFARAAGAKVIYTLRLRGGGKPEDVEPIAKYLMSHYKDEITCLAVGNE
                     PNVYAKEYPACRELITRYINAITAPDVAPDAMFCGPSTTPGKGEWAASFAKEFGPGGH
                     VKFISQHSYPGGNARKVPDPRSGRDKLLEPSIAKSYQKMYDAFVSATIEAKLPYRLEE
                     TNSYFNGGAENVSNTQMSALWALDYLHWWAAHGAAGMNFHTGDAVAAGDKPTPCWYAT
                     FWTVKDGYNVHPIGYAIKAFDIAGKGRSVPVKVESTDGVNLTGYAVAADQALYVTLIN
                     REHDENARAATITLSTIADYPHRQMMLLSAPDNDIAATSGVALGGAPIDDAANWSGKW
                     TAVSASEFEVPPSSAAVLKLSK"
     gene            3451..>4113
                     /locus_tag="VLI90_00970"
     CDS             3451..>4113
                     /locus_tag="VLI90_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSV12800.1"
                     /translation="MKQIPTRWYLWTVVAAMACAPLAARGQEEIDAGKGNPRDVAAAN
                     EALKGWWTEAAKTRDQRLEWWRDARFGCFIHWGVYSDLGGEFEGRRGGGYSEHIMRQL
                     KIPLKVYKEKVVANFDPEQFNADEWVKLIKGAGMRYVIITAKHHDGFAMWPSDAYPYD
                     IRLTKFKRDPMKELSEACHKNGLHFGFYYSHAFDWEHPDAPGNDWDYDNPGGDRSLHG
                     KDW"
BASE COUNT          764 a         1215 c         1351 g          783 t
ORIGIN      
        1 cgcgcgcggg tgggtcgttc atcatctgac ggacgtgtac ctggtgacga cgccgagcga
       61 tggcccgccg ggcgtgtggc cgatgggcgc ggcgtggatg tgccagcacc tgtgggaaca
      121 ctacgcgttc accggcgaca agcagttcct cgccaagcag ggttacccgc tgatgaaggg
      181 ggcggcccgg ttcatccttg actacatgat ccccgccccc gcgggcacgc cggtcgcggg
      241 gaagctggtg acgtgcccgt cctattcgcc ggagaacaaa ttcatcgacg ccaacggtaa
      301 gcaggcgttt ttcagctacg cctcgagcat ggacctggag atcatccacg acctgctcac
      361 gcactgcgtc gcggcggcga aggtgctcgg cagcgatgac gactttcgcg ccgagtgcga
      421 gcaggcgctg gcgaacctgg cgccgctgcg gatcgcgccg gatggtcgat tgcaggaatg
      481 gatcgaggac tacaaggaac aggacccgca tcatcgccat acgagccacc tattcgcagt
      541 gtatcccggc gatcagatta cgctggccgg cacacccgac ctggcggcag cggcgcgaaa
      601 atcgctcgag gcgcgcaccg acgaacacgc gaccgagtgg agcttcgcct ggcgctgcgc
      661 cctgtgggcc cgcttccacg acggcgcgcg cgcgtacggc caactcgaac gcctggtcag
      721 caaggacctg tacccgaacc tgtttaacaa gtacccgccg ttccagatcg acggaaattt
      781 gggcgcgacg gcagcactgg ccgagatgct gctgcaaagc caggggggcg acatccagct
      841 gctcccggcg ctacccaagg aatggccgag cggccacgtg aagggcctgt gcgcccgcgg
      901 cggctttgag gtggagatga cgtggaagga cgggacgctg caggcggcga caatcacgtc
      961 aaagatcggc ggcccgctgc gcgtgcgcgg gaacacgccg gtggtggcga aatcgactgg
     1021 ggcggaaacg gcgaaggacg gatggatcac gctggccact gaagcgggaa agacctacac
     1081 gttcgcgcgg gaataaattt cgtccaatgc ccggcgcggc ttggttgctt tagtaaccat
     1141 gtcttttgcg cctgcgcaaa ccatggtctc taaagcgacc acgccggtcg tagcgcgcgt
     1201 acgggacttc ttcgaggaac gcgttgagtt ctacagctcg cacgatggcg cgctcgatct
     1261 cgatgttgag atgcgtgtgg cgatactcgc gccaatccga gaccacgcca tgtcgttggg
     1321 cttgcaacag cgtgtaccaa taagcgcgcg ctcggcctgt agcacatctc ggatgcatcc
     1381 gtcgaagtag tcttggttgc cggcggtgcg ccagaacggg aggtggcgct gagggagaag
     1441 atcgttcacg agtttcgcca cggacccgtg aatccgctgc atcattttgc cgagatccgt
     1501 gccgcgcttc atgtagccga gcgagtggta gtggttatcc acgagcgttg tcgcccaggg
     1561 cacgaatccg aattccgcgg tgtagtgatc gaagcgatcc cagaagatct ctttggctgg
     1621 ttcagaggca aaggcatgga agcgatcgcg gcatcgcgcg gtgaggaagt aaaccgtgtt
     1681 gtcgcgatac cagtgttcga agcgatgatg cccggcgtag aactcgttgg aggtgtaaga
     1741 gctcttgcgg aagtggggca tccgcacatt ctgtcgaatt gatgcgcggc gtggttgctt
     1801 tagcagccat ggcgccagcc ctttcgaaac aaatccggac atggtcgctg aagcgaccac
     1861 gccgcgccgt catttcgaaa gctttagcac cgcggcactc gatggaggga cctcaaactc
     1921 gctcgcgctc accgcggtcc atttcccact ccaattggcc gcgtcgtcga tcggtgcgcc
     1981 gcccagcgca acgccgctcg tggcggcgat gtcgttgtcg ggggcgctca gcagcatcat
     2041 ctggcggtgc ggatagtcgg cgatagtgct cagcgtgatc gtcgcggccc gcgcattttc
     2101 atcgtgctcg cgattgatca gcgtgacgta cagcgcctgg tccgcggcga cggcgtagcc
     2161 tgtgaggttc acgccatctg ttgactccac cttcaccgga acgcttcgcc ctttgccggc
     2221 gatgtcgaat gccttgatcg cgtaaccgat cgggtggacg ttgtagccgt ccttcacggt
     2281 ccagaacgtc gcataccagc aaggcgtggg cttgtcgccc gcggcgacgg cgtcgcctgt
     2341 gtggaagttc atgcccgccg cgccgtgggc ggcccaccag tgcaggtaat ccagcgccca
     2401 cagcgcggac atctgcgtgt tgctgacgtt ctcggcgccg ccgttgaagt agctgttcgt
     2461 ttcttccagc cgatacggca gcttggcctc gatcgtcgcg gaaacgaacg cgtcgtacat
     2521 cttctggtac gacttggcga tcgacggctc gagcaacttg tcgcggccgc tcctggggtc
     2581 tggcaccttt cgcgcgttgc cgccgggata cgagtgttgg ctgatgaatt tcacgtgccc
     2641 gccggggccg aattctttgg cgaagctggc ggcccattcg cctttgccgg gcgtggtgct
     2701 ggggccgcag aacatcgcat cgggggcgac atcgggcgcg gtgatcgcgt tgatgtatct
     2761 tgtgatcagc tcgcgacagg ccggatattc tttcgcgtaa acgtttggct cgttgccgac
     2821 ggccaggcaa gtaatttcgt ccttgtaatg gctcatcaaa tacttcgcga tcggctcgac
     2881 gtcttcgggc ttgccaccac cgcggaggcg cagcgtgtag atcaccttcg cgccggcggc
     2941 gcgcgcgaac gcgatgaggc tgtccgcgtc cttctcatcc ggcacggcaa ccgcgcggtt
     3001 gtcggcggtg ttcccgccga cgcgcaggct cttgatgccc agctgcttga acagcgcgat
     3061 cagcggtttg ttgtcggcgg tgaacatccg cttgccgtcc ttgtcgggca gcagcgtttg
     3121 aatttcaaaa ctgaaaccgg tgaagtcgtc gggaatgatc gcgccggggt tggctgaatc
     3181 gatggtgacg gtgaccggcg cggcggcgct ggcgcctctt gccaggtgcg gtgaaatcaa
     3241 acagcccacg agcgcgagga tcgaccaaaa ccgggcggcg ttctgcatgc accgttcctt
     3301 tccagcccgg gcttcgccgg cgggagcggc attgtaacga gtggctccaa acgctctacc
     3361 acgcccgacc gattgtgttc aaacgaatcc tgtactgcgg cgtacaagat aaggtagact
     3421 catcctaaga aatcgtttgc aaggagcctg atgaaacaaa tcccaacccg ctggtatttg
     3481 tggaccgtcg tcgccgcgat ggcttgtgcg ccgctggcgg cgcgggggca ggaagagatc
     3541 gacgcgggca aaggaaatcc gcgcgatgtc gcggccgcga atgaggcgtt gaaggggtgg
     3601 tggaccgagg ccgccaagac gcgcgaccag cgcctggaat ggtggcgcga tgcgcgcttc
     3661 ggctgcttca tccactgggg tgtctattct gatttgggcg gcgaattcga aggccgtcgc
     3721 ggtggtggat attccgagca catcatgcgg cagctgaaga tcccgctgaa ggtgtacaag
     3781 gaaaaggtcg tcgccaactt cgatcccgag cagttcaacg ccgacgaatg ggtgaagctg
     3841 atcaagggcg cgggcatgcg ctacgtcatc atcacggcca agcatcacga cgggtttgcg
     3901 atgtggccga gcgatgcata tccgtacgac atccgcctga cgaaattcaa acgcgatccg
     3961 atgaaggaac tgtccgaagc gtgccacaag aacggcctgc actttggatt ttattattcg
     4021 catgcgttcg attgggaaca ccccgatgcg ccgggcaacg actgggacta cgacaatccc
     4081 ggcggcgacc ggagcctgca tgggaaggac tgg
//
LOCUS       DATIMH010000057         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2899717, whole genome shotgun sequence.
ACCESSION   DATIMH010000057 DATIMH010000000
VERSION     DATIMH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2899717"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="VLI90_00975"
     CDS             <1..1821
                     /locus_tag="VLI90_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HSV12801.1"
                     /translation="SMSAVGGVKGSIVGNVIAGNRSLGSSPYGVGFVIANTAPGSPTL
                     VADNVFTGDSQHASPAMTLQAAWSTKNPAAAVGINDVIIQSNVFNGWFRAIQTDGGLV
                     PGGSGLAALNRVFIRNNEFQATSGIVVRHDGPFDPTNETWQQNRYFDTPADGKWFVLG
                     QHNITFANWQAQVDPTGVLEQDVFPDDSRNAGTYDGSIGGAGTVDSFVAAERLQSSDH
                     WDPRYIATSINDYIREGFNAATAPIAAATTSPVDMQTVGTPPPPAMVSANVVSAASDA
                     PTAADDLYTFTVAYADTQALDAAGIGSGNVDVVGPNGFDESATLVSMTQAADGKTADA
                     TYSITPPGGSWDGADDGQYAVMMQPGQVRNKAGVGVPEGAIGAFGVSLPLDIVGTAGN
                     DRIGMVALPKNRLLVVVNGKRTIYSLSNVPEVRVFGGDGNDLIVAGTGAPSCYIDGGA
                     GNDRVRGGPGNDTILGGDGNDLIGDVAGQNILIGGAGNDRIVGGVGNDVISGGDGDDI
                     LIGGAGDDVLIGGNGFDRMAGVAGNDLLIGGASSSDDPTALAAMRDSWSALDDAGKTA
                     TLAQPTFMIGNAAFSDDGIVDRLYGGAGNDWFTATDQIVQ"
     gene            1951..2033
                     /locus_tag="VLI90_00980"
     tRNA            1951..2033
                     /locus_tag="VLI90_00980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1985..1987,aa:Leu,seq:cag)
     gene            complement(2070..3119)
                     /locus_tag="VLI90_00985"
     CDS             complement(2070..3119)
                     /locus_tag="VLI90_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSV12802.1"
                     /translation="MTPPGAAGIAVLRLVGPGVEPFLRQRFSKPVQVGRCVHGELRDG
                     DRVIDDPVVVLCRADVADVNLHGGVWVVRAAIELAKRDGFEVVAAVDDVSPVEAVDAQ
                     TVVERDVLRWLPRATTDLAVRALLAQPAAWEQMLARAARSEVGPEQIDRIIGDQSLHW
                     LLSQPRVAIVGSPNVGKSTLANQLFGQVRSITADVAGTTRDWIGEVANIDGLAVMLLD
                     TPGRRETADPIERAAIEVSADEISAADLVVIVLDHSAELGGVEQALLTAHPTAIVVAN
                     KSDLPHRWHTSDVGAIETVASDARGVDQLRRAILRHFSCDDLSLDVPRWWTAEQRQLL
                     RRAVDDPALLAEMAA"
BASE COUNT          595 a         1104 c         1024 g          540 t
ORIGIN      
        1 agcatgagcg cggtgggcgg ggtgaagggg tcgatcgtcg gcaatgtcat cgcggggaat
       61 cggtcattgg ggtcgtcgcc gtatggggtg ggattcgtga tcgccaacac cgcgccgggc
      121 tcaccgacct tggtggcgga caacgtcttc accggcgatt cgcaacacgc cagccccgcc
      181 atgacgctgc aggcggcgtg gagcacgaag aacccggccg ccgctgttgg catcaatgac
      241 gtcatcatcc aaagcaacgt tttcaacggc tggttccgcg cgatacagac cgatggcggg
      301 ctcgtgcccg gcggcagcgg actggccgcg ctcaaccgcg tcttcatccg caacaacgaa
      361 ttccaggcga ccagcggcat cgtcgtgcgg cacgatggcc cgtttgatcc gacgaacgaa
      421 acctggcagc agaaccgata tttcgacacg cccgccgatg gaaagtggtt cgtgctcggc
      481 cagcacaaca tcacgtttgc taactggcag gcgcaggtcg atccgaccgg cgtgcttgaa
      541 caggatgtgt tccccgatga cagccgaaac gccggcacgt acgacggcag catcggtggc
      601 gctggcacag tcgattcgtt cgtggcggcc gagcgattgc agtcgtcgga ccattgggac
      661 ccgcgctaca tcgcgacctc gatcaacgat tacatccgcg aaggcttcaa cgccgccacc
      721 gcgccgatcg cggcggcgac cacgtcgccg gtggatatgc agaccgtcgg caccccaccc
      781 cccccggcga tggtgagcgc gaacgtcgtc agcgcggcgt cggatgcgcc aactgccgcg
      841 gacgatctct atacgttcac cgtcgcgtac gccgatacgc aggcgctgga tgccgcgggc
      901 atcggcagcg ggaacgtcga cgtcgtcggg ccgaatggtt ttgacgaatc tgcgacgctg
      961 gtgagcatga cgcaggcggc cgatgggaag accgcggatg cgacctattc gatcacgcca
     1021 ccgggcggat catgggacgg cgctgatgac gggcagtacg ccgtgatgat gcagccgggc
     1081 caagtgcgca ataaagccgg cgtgggtgtg ccggaaggcg cgatcggcgc gtttggcgtg
     1141 tcgctgccgc tcgatatcgt cggcaccgcc ggcaacgatc gcatcggcat ggtggcgctg
     1201 ccgaagaatc ggctgctggt ggtcgttaac ggcaaacgca cgatctacag cctaagcaac
     1261 gtgccggagg tccgcgtctt cggcggcgac ggcaacgacc tgatcgtcgc cggcacgggc
     1321 gcccccagct gctacatcga cggcggcgcc ggcaacgacc gcgtccgcgg cgggcccggc
     1381 aatgacacca ttctcggcgg cgatggaaac gatttgatcg gcgacgtcgc cggtcaaaac
     1441 atcctcatcg gcggcgccgg caacgaccgc atcgtcggcg gtgtcggcaa cgatgtgatc
     1501 agcggcggcg acggcgatga catcctcatc ggcggcgcag gcgacgacgt cctgatcggc
     1561 ggcaacggct tcgaccgcat ggccggcgtc gccggcaacg acctgctcat cggcggcgcc
     1621 agttcgagcg atgacccaac ggcgcttgct gcgatgcggg acagttggtc cgcgctggac
     1681 gacgccggca agactgcgac gctcgcgcag ccgacgttca tgattggaaa cgccgcgttc
     1741 agcgacgacg gcatcgtcga ccggctatac ggcggcgccg gcaacgactg gttcaccgcg
     1801 accgaccaga tcgtgcaata agcaacgcgt ttagccgccg cggcctcgcg gcgcctttct
     1861 acgtccgcgc cgcgaggccg cggcggctaa tcgtggagac gctgtcgcgc ttgactccgc
     1921 ccgtccgcca tttagaattc tccatccgtc gcggctgtgg cggaattggc agacgcgcta
     1981 gattcaggtt ctagtgtcca caagacgtgc aggttcaact cctgtcagcc gcatgaaaaa
     2041 ccccggttct gccggggttt ttgcgtttat taagccgcca tctccgcaag cagcgctggg
     2101 tcatcgactg cccgccgcag cagctgacgc tgctccgcgg tccaccatcg cgggacatcg
     2161 agcgacaaat catcacaact gaaatgtcga aggatcgccc gccgcagttg atcgacgcca
     2221 cgggcatcgc tggcgaccgt ttcgatcgcg ccaacgtcac tcgtatgcca tcggtgcggc
     2281 aagtctgatt tgttggcgac gacgatcgcc gtgggatgag cggtgagaag cgcttgctcg
     2341 acgccgccca actcggcgga atgatcgagc acgattacca ccaaatccgc cgccgagatc
     2401 tcatcggcgc tcacttcaat cgcggctcgc tcgatcgggt ccgccgtctc ccgccggccg
     2461 ggcgtatcca gcagcatcac cgccaacccg tcgatgttgg caacttcacc gatccagtcg
     2521 cgcgtcgtcc cggccacgtc ggcggtgatc gatcgcactt gcccgaatag ttgattcgcc
     2581 aacgtggact tccccacgtt cggcgagccg acaatggcga cgcgcggctg cgacagtagc
     2641 cagtgcaggc tctggtcgcc gatgatccga tcgatctgct cgggccccac ctcgctgcgc
     2701 gccgcccggg ccagcatttg ttcccaagcc gccggctgcg ccagcagcgc gcggaccgcc
     2761 aaatccgtcg tcgcgcgcgg cagccaccga agcacatcgc gctcgaccac cgtttgagca
     2821 tccaccgctt cgaccggcga aacatcatca accgctgcga cgacttcgaa accgtcgcgc
     2881 ttcgccaatt caatcgcggc ccgcaccacc cacacaccgc cgtgcaggtt cacatccgcg
     2941 acatccgccc ggcacagcac gacgaccggg tcgtcgatca cgcgatcgcc atcgcgcagc
     3001 tccccatgca cacatcgccc cacttggaca ggtttcgaaa accgttgccg caggaatggt
     3061 tcgacgcccg gcccaaccag ccgcaacacc gcgatccccg ccgcccccgg cggggtcatt
     3121 tgcagcactc ggttcatccg ttgcgcattt gccacgatcg tttagtagtc tgcgaagcgt
     3181 gggttgtcga acatctctgt cgctgcgacg ccgtcgtcgc cctggcgccg cgatggcggc
     3241 ggtatcttag acagaagtaa atc
//
LOCUS       DATIMH010000058         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_301036, whole genome shotgun sequence.
ACCESSION   DATIMH010000058 DATIMH010000000
VERSION     DATIMH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301036"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VLI90_00990"
     CDS             complement(<1..825)
                     /locus_tag="VLI90_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HSV12803.1"
                     /translation="MRKMLFVIALMLAPAVARANLKIVTTATDFTDIARAIGDGKVDV
                     HSVMKGPENIHNVIPKPTEMSALNEADLFVHSGLDAEPWRDNLLAGARNPKVEANKPG
                     NVDMSQGITIKDAPVGKIDRSMGDVHAYGNPHYTLNPKIAQRMALTLYLAMAKADPAN
                     AALYKENAVKFVKEMGEVSKQIREEFKPYDGLKVMTFHDAWKYFADAIPLEVVGTIEP
                     KPGITPSPAQVQQEIDTMKQKGVKVVICETWDDVKLAQYVADQAGAKMVLLPDHVNG"
     gene            complement(909..>2242)
                     /locus_tag="VLI90_00995"
     CDS             complement(909..>2242)
                     /locus_tag="VLI90_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12804.1"
                     /translation="PPATQPTDARMDRFEQRLNEMDQKYQAELKARDAKISQLEAELA
                     ARPAAPAATQPAAVPATDATLNDIDKSTAEMLKDIETQQAPPSAPRLPASFNPDIAVI
                     GDFAGNASTYTRNPARNHFDLREVELDLRAAVDPRAEAVVILPIDRSVPDPLFFNPAT
                     ADGHVDTSVDIEEAFLHLHDFGVPNLSADLGRFHMHFGRWNLLHLHAWPTVDNAFMVQ
                     NFLGPESLVDSGLRLDYLVPRQWVGGQTIEAFAEVVTGMGDSEDPVLNNDGFVNSPAL
                     NSHLLWNHDLTPNWNFELGGSFLWAHHNDAPNQYATLTGIDFTLMHTDPMGRYNNQVF
                     QVEWIYGNVDTSPTATQHSNGGFVLLQQQVNADWYVGARLDWTKNAVNQHQEVWDVAP
                     YATWYWSEFLRFRVEYQHKDGNGSPREDNVWFQVDFVFGAHPPHPYWSSLH"
BASE COUNT          367 a          689 c          724 g          462 t
ORIGIN      
        1 gccgttcacg tggtcgggga gcaggaccat tttcgcgccg gcctgatccg cgacgtactg
       61 ggccagcttc acgtcgtccc atgtttcgca gatgacgact ttcacgccct tctgcttcat
      121 cgtgtcgatc tcctgctgca cctgcgcggg cgacggcgtg atgccgggct tgggctcgat
      181 ggtgccgacg acttccagcg ggattgcgtc ggcaaaatac ttccacgcgt cgtggaaggt
      241 catcaccttg agcccgtcgt acggcttgaa ctcctcgcgg atctgcttgg agacctcgcc
      301 catctccttc acgaatttca ccgcgttctc tttgtacaag gccgcgttgg ccggatcggc
      361 cttggccatc gccaggtaca gcgtcagggc catccgctgg gcgatcttgg ggttgagggt
      421 gtagtgcggg ttgccgtagg cgtgaacgtc gcccatggag cggtcgatct ttccgaccgg
      481 cgcgtccttg atcgtgatgc cctggctcat gtccacgttg ccgggcttgt tcgcttcgac
      541 cttcgggttg cgggctccgg ccagcaagtt gtcccgccac ggctcggcat cgagcccgct
      601 gtggacgaac aggtcggcct cgttcagcgc gctcatctcg gtgggcttgg ggatgacgtt
      661 gtgaatgttc tccggcccct tcatgaccga gtgaacgtcc acctttccgt cgccgatcgc
      721 gcgggcaatg tcggtaaaat ccgtcgcggt cgtgacgatc ttcaagttcg cccgcgcgac
      781 tgccggggcg agcatcaacg caatcacaaa caacattttt ctcatgacaa actcctaact
      841 tgcggggtcc gctcagcgga cggaatcggc gccatcgcgt ccgctgaagc ggaccctgcg
      901 caatgatttc aatgaagcga tgaccagtac gggtgcggcg gatgcgcgcc gaagacgaaa
      961 tccacctgga accagacgtt gtcttcgcgc ggcgagccgt tgccgtcctt gtgctggtac
     1021 tccacgcgga accgcaggaa ctcactccag taccacgtcg cgtacggcgc gacgtcccac
     1081 acttcctggt gctggttgac ggcgttcttc gtccagtcga ggcgggcgcc gacgtaccag
     1141 tcggcattga cctgctgctg gagcaggacg aaaccgccgt tggagtgttg cgtggcggtg
     1201 gggctcgtat cgacgttgcc gtagatccac tcaacctgaa agacctggtt gttgtaccgg
     1261 cccatggggt cggtgtgcat gagcgtgaaa tcgatgccgg tcagggtggc gtactggttg
     1321 ggcgcgtcgt tgtgatgagc ccagaggaac gacccgccca gctcgaagtt ccagttcggg
     1381 gtgaggtcgt ggttccagag caggtgtgaa ttcagtgccg gggaattgac aaacccgtcg
     1441 ttgttcagga ccggatcctc cgagtcgccc attccggtga cgacttctgc aaacgcctcg
     1501 atcgtttgcc cgccgaccca ctgccgggga acgaggtaat cgagccgcaa ccccgaatcg
     1561 accagcgatt cggggccgag aaaattctgc accatgaacg cgttgtccac cgtcggccag
     1621 gcgtgcaggt ggagcaggtt ccaccggcca aagtgcatgt ggaaccgtcc gagatccgcc
     1681 gagaggttgg gcacgccaaa gtcgtgcagg tggagaaaag cctcctcgat gtcgactgac
     1741 gtatcgacgt gcccgtcggc ggtggccgga ttaaaaaaca gagggtcggg cacgctgcgg
     1801 tcgatcggca gaatgacgac cgcctcggcc cgcgggtcca ccgccgctcg caggtcgagt
     1861 tcgacttcgc gcaggtcgaa atgatttcgc gccgggttgc gcgtgtacgt cgaggcgttg
     1921 cccgcaaagt cgccgatcac ggcgatgtcg ggattgaaac ttgccggcag ccggggcgcc
     1981 gaaggcggcg cctgctgcgt ctcgatgtcc ttgagcatct cggcggtgct cttgtcgatg
     2041 tcgttcagcg tcgcatccgt cgccggaacc gcggcgggct gcgtcgccgc cggcgcggcg
     2101 ggacgcgccg ccagttccgc ttcgagctgc gaaatcttcg catcccgcgc tttcagctct
     2161 gcctgatatt tctgatccat ctcattgagc cgctgctcga agcggtccat ccgcgcgtcg
     2221 gtcggctgag tggccggggg at
//
LOCUS       DATIMH010000059         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3189505, whole genome shotgun sequence.
ACCESSION   DATIMH010000059 DATIMH010000000
VERSION     DATIMH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3189505"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VLI90_01000"
     CDS             complement(<1..253)
                     /locus_tag="VLI90_01000"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSV12805.1"
                     /translation="MDASFLATEFSAALSYADYLRTGTEEQQRRWQQAYDVARLSEAQ
                     QDLVAGFVRSMNVLVVSGIWCGDCVQQCPLMQRIAEANAA"
     gene            complement(408..>2334)
                     /locus_tag="VLI90_01005"
     CDS             complement(408..>2334)
                     /locus_tag="VLI90_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12806.1"
                     /translation="LESPPLMALTAGQAAVQFVGRPVELTPVGSTVHAAVVTYRNDGA
                     VRLENSPALPIVEMRQDRGMMFDAESIQYDPATQLATILGRSDARMPTARGAQELTAS
                     WTRRGVLHFTGNPAQPDAVDRADLDGHVSVEHPSFNLKSNQLALDMEKIAVPATRPGE
                     VAGSDVAVKRATAVGDVQCRLLRPGQADRGIDGDRLVLETAPGADGKRVPRSVVADGN
                     VHAIDVKQEIFADHLDALIRPKPAPAVADKKAKQPANDEDALDLESMYASKNVHAKLK
                     SGAMADADELRVTTTTNDKQKIELRGQPTASIGDGKGSTLSGPVLHVSPDPLLVTVDG
                     PGTMRKMRRATTTQPAQPMDVSWTDSMTYDGTGNMIDVIGHVAVAMTDKSGTVDTMHG
                     DTAHLELVDVKPAKPEKRAKPGAAPTTRPESDFGDKELKSMLLQGHVHGESELKDANG
                     APLRRGAVVGDRLTYDAEKGRTEIPGPGWLEMEDHRSTKAGGNRGAMAIQWKTGLLYN
                     KPANQVVITGDTLVGFRKDAKDAAPMQLKAQRLTIDVQPEKAGAPADASIQISHVRAE
                     ENVDMFLPGKGTHFMGHSVDYDPAKEEVTLRGSAQEPGRVLDEQGASTGTFDELVIDT
                     RTQEVDRTRGANLNMRK"
BASE COUNT          350 a          828 c          699 g          457 t
ORIGIN      
        1 tcgccgcgtt ggcctcagca atgcgctgca tcagcgggca ctgctgcacg cagtcgccgc
       61 accagatgcc gctgacgacc agcacgttca tcgagcgaac gaacccggca acgagatctt
      121 gctgagcctc gctcaggcgc gcgacgtcat acgcctgctg ccagcggcgc tgctgttcct
      181 cggtgccggt gcggaggtaa tcggcgtagc tcaaagcggc ggaaaattcg gtcgcgagaa
      241 atgatgcgtc catggtgatg ctccgaacga atgatggtta acaaataaag tggcgccacc
      301 gacaaagccc ctggctctgc caggggacgt cttcgataac cacatcgcgc gctgttcgaa
      361 aggagtccta cggcaaagcc gtgggcctta ggcaattcat caatcgatca cttgcgcatg
      421 ttcaaattcg cgcccctggt ccgatcgacc tcctgcgtcc gcgtgtcgat caccagctca
      481 tcgaacgtcc ccgtcgatgc cccctgctca tccagcacgc gccccggctc ctgggctgat
      541 ccgcgcagcg tcacctcttc cttcgccgga tcgtagtcaa ccgagtggcc catgaagtgc
      601 gtgcctttgc cggggaggaa catgtccacg ttttcctcgg ctcgcacgtg cgaaatctgg
      661 atgctcgcat ccgccggcgc tccggctttt tccggttgca cgtcgatcgt caggcgctgc
      721 gccttgagct gcatcggcgc tgcatccttc gcgtctttgc ggaagccgac cagcgtgtcg
      781 ccggtgatga cgacttggtt cgcgggcttg ttgtaaagca aaccggtctt ccactggatc
      841 gccatcgcgc cgcgattccc gccggccttg gtggaccgat ggtcttccat ctccagccaa
      901 ccgggcccgg ggatttccgt ccgccccttc tcggcgtcgt acgtaagccg gtctccaacc
      961 actgccccgc ggcggagcgg tgcgccattg gcgtctttta gctccgactc gccgtgcacg
     1021 tggccctgca gcagcatcga cttcagttcc ttgtcaccaa aatcggattc cggccgagtc
     1081 gtcggcgctg cccccggctt ggcgcgcttc tcaggtttgg cgggtttcac atcgaccagt
     1141 tcgagatgag ccgtgtcacc gtgcatcgta tcgacggtgc cgctcttgtc cgtcatcgcc
     1201 accgcgacgt gaccgatcac gtcgatcatg ttgccggtgc cgtcgtacgt cattgagtcg
     1261 gtccacgaca cgtccatcgg ctgagccggt tgcgtcgtcg tcgcacgccg catcttccgc
     1321 atcgtgccgg ggccatcgac ggtgaccagc agcggatccg gcgacacgtg cagcaccggc
     1381 ccgctcagcg tgctgccctt gccgtcgccg atgctggccg tcggttgccc gcgcagctcg
     1441 atcttctgtt tgtcgttcgt ggtcgtcgtc acgcgcagct cgtcggcgtc ggccatcgcc
     1501 ccgctcttga gcttcgcgtg cacgtttttg ctggcgtaca tcgattccaa atccagcgca
     1561 tcctcgtcat tggccggctg cttcgccttc ttgtccgcca cggccggcgc cggcttgggg
     1621 cggatcagcg cgtccaggtg atcggcgaaa atctcctgct tcacatcgat cgcatgcacg
     1681 ttgccgtcgg cgaccacgct gcgcggcacg cgcttcccgt cggcgcccgg cgcggtttca
     1741 agaacgagcc gatcgccgtc gatcccgcgg tccgcctggc ccggacgcag caaccggcac
     1801 tgcacatcgc ccaccgcggt cgcgcgcttc acggcgacat ccgaacccgc gacctcgccc
     1861 ggccgggtcg cgggcaccgc gatcttctcc atgtccagcg ccaactggtt cgacttcaga
     1921 ttgaagctgg gatgctcgac gctgacgtga ccgtcaagat cggcgcgatc gaccgcatcg
     1981 ggttgagccg ggttgcccgt gaagtgcagc acgccccggc gggtccacga tgccgtcaac
     2041 tcctgcgcgc cgcgcgcggt cggcatgcgc gcgtcgctgc ggcccaggat ggtcgccaac
     2101 tgcgtcgccg ggtcatactg aatcgactcg gcgtcgaaca tcatcccgcg gtcctgccgc
     2161 atctcgacaa tcggcagcgc cggcgaattc tccagccgca ccgcgccgtc gttgcgatag
     2221 gtgacgaccg ccgcgtggac ggtcgatccc accggcgtca gttccacagg ccggcccaca
     2281 aattgcaccg ccgcctgccc cgcggtcagc gccatcagcg gcgggctctc cagc
//
LOCUS       DATIMH010000060         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4922031, whole genome shotgun sequence.
ACCESSION   DATIMH010000060 DATIMH010000000
VERSION     DATIMH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4922031"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VLI90_01010"
     CDS             complement(<1..824)
                     /locus_tag="VLI90_01010"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HSV12807.1"
                     /translation="MPLASAAPRVINIGPNGTSDNGMRLVKTRHYEIHTDLDAALVTD
                     LANRMDVMYDEYTQRLREFHPPTDAPAMPVYLFTHEADYAALTGNMGTNTGGIFVFGG
                     KKTYLAAFLGDQGRDKLRHTLQHEAFHQFAYFAISHDLPIWLNEGLAQFFEEGIWTGK
                     NFWINQVPPERLWDLQDQIKKHKLIEFQRFLDVTPDQWHKNLSGSLAAGDLYYQQAWA
                     MVQFLVDGSHPDYRTRLIDYLKRLHNNESPDKAFEAAFSKNIKGFQDRFAEWIVNL"
     gene            1079..>2332
                     /locus_tag="VLI90_01015"
     CDS             1079..>2332
                     /locus_tag="VLI90_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HSV12808.1"
                     /translation="MSEEPSHYEQERRQKRDKLRELGIDPYGGREVGVQPLAAIKSSY
                     KPEMGHDGGPVVKAAGRVMLKRDMGKLSFLTLRDESGDLQVALDKKRLDERAWEVNGL
                     IDLSDQIVVEGPLGVTKKGEVTIWATQLRMASKALLPPPAKWEGLSDIELRYRQRYVD
                     TWANPDVLRVLKLRMQIVDEVRQYMRDRGYLEVETPMMQTLAGGAAARPFVTHHNALD
                     IPLYLRIAPELYLKRLLVGGFSKVFELNRNFRNEGISPRHNPEFTMLEAYEAYGSWET
                     MADLVEGMVCHIAEKLFGGLKLVHHGDTETRREINLQRPWRRVRMVDLVAERTGWHFN
                     KEPLTAEQTEKLRAKNPGKDLKLKGSPAEQMVEVYEKLIEHTLIDPTFVTHVPSVIIP
                     LARKNQDDPFFADVYELAINGQEISP"
BASE COUNT          484 a          667 c          768 g          413 t
ORIGIN      
        1 ttgaggttga cgatccactc ggcgaagcga tcttgaaagc ccttgatgtt cttcgagaac
       61 gcggcttcga atgccttgtc gggcgattcg ttgttgtgca ggcgcttgag gtagtcgatc
      121 aagcgcgtgc gataatcggg atggctgccg tccaccagga actgcaccat cgcccacgcc
      181 tgctgatagt acaggtcgcc ggcggccagg ctgccggaga gatttttgtg ccactggtcg
      241 ggcgtgacgt cgaggaacct ctggaactcg atcagcttgt gcttcttgat ttgatcctgc
      301 aggtcccaca gccgctcggg cggcacctgg ttgatccaga aattcttgcc ggtccagatg
      361 ccttcctcga agaactgcgc gagcccttcg ttaagccaga tgggcaggtc gtggctgatg
      421 gcgaagtatg cgaactgatg aaacgcttcg tgctgcagcg tgtggcggag cttgtcgcgg
      481 ccctggtcgc cgaggaacgc ggcgaggtat gtcttcttgc cgccaaagac aaagatgccc
      541 ccggtgttgg tgcccatgtt cccggtgagg gcggcgtagt cggcctcgtg cgtgaagaga
      601 tacaccggca tcgccggggc gtcggtggga ggatgaaact cgcgcagccg ctgggtgtac
      661 tcgtcgtaca tcacgtccat gcggttggcc agatcggtga ccagcgcggc gtcgagatcg
      721 gtgtgaatct cgtaatgacg cgttttgacg aggcgcatgc cgttgtcgga ggtgccgttg
      781 ggcccgatgt tgatcacgcg cggcgccgca gacgcaagcg gcacaacaat acaaaaggcc
      841 gccacgaacc cgtgccagca gagagcccag cggctccgcc gcgggacgcg cgggagtcga
      901 aaagacatca ctcgcgaaaa cagcgcacat cgcaacgcgc cccgcggcgg agccgctggg
      961 ctctgtgctg gcgcgggctt ggatgggcct ggcgagaggg cgcttcgttt gtcagtcatg
     1021 ggcgtttcca ctttcggtgc ggtcccacta tcatcggcaa actttttcag aaccacaaat
     1081 gtcagaagaa cccagtcatt acgagcagga acgccggcag aagcgcgaca aactgcgcga
     1141 actcggcatc gatccatacg gcggccggga agtcggcgtt cagccgctgg cggcgatcaa
     1201 gtcatcgtac aaacccgaaa tgggtcacga tggcggaccg gtcgtcaagg cggcaggacg
     1261 cgtgatgctg aagcgcgaca tggggaagct cagcttcctc acgctgcgcg acgagagcgg
     1321 cgacctgcag gtcgcgctcg acaagaagcg gctcgacgag cgcgcgtggg aagtgaacgg
     1381 cctgatcgac ctgagcgatc aaatcgtcgt cgaaggcccg ctcggcgtta ccaaaaaggg
     1441 cgaagtcacc atctgggcga cgcagttgcg catggcgagc aaggcgttgc tcccgccgcc
     1501 ggcgaaatgg gaggggttga gcgatatcga gctgcgctat cgccagcgct atgtggacac
     1561 ctgggccaac cccgacgtgc tgcgcgtgct gaagctgcgg atgcagatcg tggacgaagt
     1621 gcgccagtac atgcgcgacc gcggctacct ggaagtcgaa actccgatga tgcaaaccct
     1681 cgccggtggc gccgcggcgc gaccgttcgt cacgcaccac aacgcgctgg acatcccgct
     1741 gtaccttcgc atcgcccccg aactgtacct gaagcggctg ctggtcggcg ggttcagcaa
     1801 ggtcttcgaa ctgaatcgca acttcagaaa cgaaggcatc agcccgcggc acaatcccga
     1861 gttcacgatg ctggaggcgt acgaagcgta cgggtcgtgg gagacgatgg ccgatctggt
     1921 cgaaggcatg gtgtgccaca tcgcggagaa gctcttcggc gggttgaagc ttgttcacca
     1981 cggagacacc gagacacgga gagaaattaa tttgcagcga ccttggcggc gcgtgcgcat
     2041 ggttgacctc gtggccgagc gcaccggttg gcatttcaat aaggaaccgc tgacggcgga
     2101 gcagaccgag aaactgcggg cgaagaatcc ggggaaagac ctgaagctga agggatcgcc
     2161 agcggagcaa atggtagaag tgtacgagaa gctgatcgag cacacgctga ttgatccaac
     2221 cttcgtcacg cacgtgccgt cggtcatcat cccgctggcc cggaaaaacc aagacgaccc
     2281 gttttttgcg gacgtgtatg aactggcgat caacggccag gaaatcagcc cc
//
LOCUS       DATIMH010000061         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_879369, whole genome shotgun sequence.
ACCESSION   DATIMH010000061 DATIMH010000000
VERSION     DATIMH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879369"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VLI90_01020"
     CDS             <1..988
                     /locus_tag="VLI90_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HSV12809.1"
                     /translation="GDGIGPEVTAAGRRVLEAAGLSVDWVVLPAGTSAIEQGFENVLP
                     QRTLAAIQAHKVALKGPVTTPVGKGFKSVNVQLRQKLNLYAAVRPVRNLPGITTRFNN
                     VDMVIVRENTEGLYSGIENEVVPGVVTSLKVASHAACTRIARYAFRYATRRHRKKITV
                     FHKANIMKLSDGLFLDCARRIYEEEYPNIEYEELIIDAGCMKMVQDPTRFDVLLMENL
                     YGDLMSDLCAGLVGGLGVVPGSNIGDDAAVFEAVHGSAPDIAGKGIANPLAIIMSGVM
                     MLNHLHEEAIATKIKNAYNAVLEESDKAKLTKDLGGSATTDQFADALIAKMK"
     gene            1336..>4340
                     /locus_tag="VLI90_01025"
     CDS             1336..>4340
                     /locus_tag="VLI90_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12810.1"
                     /translation="MNASSKVRAASGFGVGSRRRTPVSSAAGHAVEALEGRRLLSANA
                     WKAAVSGDWDTASNWSAGHVPTASEDVSIAIAGNYTVTHSFGTDSAHSVTLIHPLTIS
                     GGVLTVATTVQANSTFTLSGGTLGNATINAGAGGHFIPTSGTLNHVTLGADVTMANGN
                     QLTIVKGLSLGGHTLTLSSGGSYTGLIFNDAAAQSLSGPGKVVFAGTTPNTDFFNTYG
                     SAAAPVTLAASISANGGIIQSLAGVTGALSIQAPITMASAGNTLTLNTFTNKSTMTVS
                     AGAITFGGAWVNQGTVAVTNAAATLNLGGTFGAIGTINHSAGTVNLTGTDTVNGTLAL
                     TAATGSWNLAGGTLINATLSFAGGAALVPTSSGGTFNHVTLGSDLSFPNNGQITVIKG
                     LSLNSHKVTLASTSAYTGIIFNDSAAQTLSGPGQVLLAGSSPSTDFFNCYGGASNPLT
                     LAANVTINASGGLLQGLGGPASGFVIKSAINMSSAAGNLSISSFTNQGTLTDSAGTVT
                     LGGPWANSGTIVVNNAAATLNLGGTFSKVGTINHSAGTVNLMGTGTINGTLAFTAATG
                     SWKMAGGTLVNATLSFAGGASLVLTSSGGTFNHVTLGSDLTFGNNGQITVVKGLLLNS
                     HTITLASTAAYTGIIFNDSAAQSLSGPGQVIFAGTSPNTDFFNCYGAAAIPVTLGSGV
                     TISAAGGLIQNLGGATAGLINAAAITVTTGTLTLNGSLTNKGTLFAKGGNIVDNGAEL
                     VNSGTILVATGHSLTMAVPTATLTNSGGGVLSGTGTIVAPTVINSATVSPGAPLTPGT
                     AAAGKLTITGNFTQTASGTLRAELGGTAGGAFDVLAVSGTAALAGLLDVYTFNGFHPA
                     KGNVFGVMTYKSHTGAFTSTDGLSIGNGVALTAAATGTRETLTGVTMPADATAPTVSA
                     PTLVGTPAKGGTTLQFKMTFSDNIAIDIATLGNGDLLVTGPNGFSQLATFVSVSKASN
                     GSPRTAIYQLAAPGGTLDALDSGTYTAAV"
BASE COUNT          831 a         1436 c         1366 g          707 t
ORIGIN      
        1 cggcgacggc atcggtcccg aagtcaccgc cgccgggcgg cgcgttcttg aagcggcggg
       61 gctttccgtc gattgggtcg tcctgcccgc ggggaccagc gcgatcgaac aggggttcga
      121 gaacgtcctg ccgcagcgca cgctggcggc cattcaggcg cacaaggtgg cgctgaaggg
      181 gccggtgacc acgccggtgg gcaagggctt caagagcgtg aacgttcagc tgcggcagaa
      241 gctgaacctg tacgcggctg tgcggccggt gcgcaatctg cccggcatca ccacccgctt
      301 caataacgtg gacatggtca tcgtccgcga gaacaccgag gggctataca gcgggatcga
      361 aaacgaagtg gtccccggcg tggtcaccag cctgaaggtc gccagccacg ccgcgtgcac
      421 gcgcatcgcg cgatacgcct ttcgctatgc gacccggcgg catcgcaaga agatcaccgt
      481 cttccataaa gcgaacatta tgaaactgtc cgatggcctg ttcctggatt gcgcccgccg
      541 gatctatgaa gaggaatatc caaatataga atatgaagag ctgatcatcg acgcgggctg
      601 catgaagatg gtgcaggacc cgacgcgctt cgacgtgctg ctcatggaga atctgtacgg
      661 cgacctgatg tccgacctgt gcgccggcct ggtcggcgga ttgggcgtgg tccccggcag
      721 caacatcggc gacgacgccg ccgtcttcga agccgtgcac ggctccgccc ccgacatcgc
      781 cggcaaagga attgccaacc cgctggccat catcatgtcc ggcgtgatga tgctgaacca
      841 cctgcacgaa gaagcgatcg cgacgaagat caagaacgcg tacaacgcgg tgctggaaga
      901 gagcgacaag gcgaagctga cgaaggatct gggcgggtcc gctacgaccg atcaattcgc
      961 ggatgcgttg attgcgaaga tgaagtgaga tgggcgggtt actcgatcga agaggtaatt
     1021 gctgccacga aatcccccgt cggacgatca gctttaggtc cgcggtcgat gcaacaccga
     1081 aagatgttct tctcgatgcc gctcggaatt tagatatacg caagtgatga gactcggacc
     1141 gggctgggga cggcccggct ttcccgcttg gaattgccgg aattcctttg cttccgcggg
     1201 tcgctcggtc taccttggca aattcctggt atggggttat gcgcgataaa ttggcgcgct
     1261 cggcgctcgt tcgcgcgatg cgagtacata catctcttgc ggtgatcacg tccccggcaa
     1321 gcgaaggaga ggaagatgaa cgcttctagc aaggtgcgcg ctgcgtctgg gtttggtgtt
     1381 gggtcgcgcc ggcggacgcc ggtcagctcc gccgcgggcc atgcggtgga agcgctggaa
     1441 ggccggcggc ttttgagcgc caacgcctgg aaggccgcgg tcagcggcga ctgggatacg
     1501 gccagcaatt ggtccgcggg gcatgtgccg acggcgagcg aggatgtttc gatcgcgatc
     1561 gccgggaact acaccgtcac acattcgttc ggcaccgact cggctcacag cgtgacgctg
     1621 atccatccgc tgacgatctc gggcggcgtg ctcaccgtcg ccaccaccgt gcaggcgaac
     1681 agcacgttca cgctgagcgg cggcacgctg gggaacgcga cgatcaacgc cggcgcgggc
     1741 ggtcatttca ttccgacgag cggaacgctc aaccacgtca cgctgggcgc ggacgtgacg
     1801 atggccaacg gcaatcagct gacgatcgtc aaaggcttgt cgctcggcgg tcacacgctg
     1861 acgttgagca gcggcggcag ttacacggga ttgatattta atgacgccgc ggcccagagt
     1921 ctctcggggc cgggaaaagt tgtcttcgcg ggaacgacgc cgaacacgga cttcttcaac
     1981 acctacggct cggcggcagc gccggtcacg ttggcggcca gcatctcggc caatggtggg
     2041 atcatccaga gcctggccgg cgtcactggc gcgttgagca tccaagcgcc gatcaccatg
     2101 gcgtcggccg gcaatacgct gaccctcaac acattcacga ataaatcgac gatgacggtc
     2161 agcgcgggcg cgatcacctt cggtggcgca tgggtgaacc aggggacggt cgccgtgacc
     2221 aacgcggcgg ccaccctgaa tttgggcggc acctttggcg ccattggcac gatcaaccac
     2281 agcgccggca cggtgaacct caccggcacc gacacggtca acggcacgct ggcgctgacg
     2341 gctgccaccg gcagctggaa cctcgcgggc ggcacgctga tcaacgcgac gttgagcttc
     2401 gccggcggcg ctgcgctggt gccgacgtcc agcggcggaa cgttcaatca cgtcacgctc
     2461 ggctcggacc tgtcgtttcc gaacaacggg caaatcaccg tcatcaaggg cctgtcgctg
     2521 aacagccaca aggtcacgct ggccagcacg agcgcgtaca cgggcatcat cttcaacgat
     2581 tcggcggcgc agactctgtc tgggccgggt caagtccttc tcgccggcag ttcgcccagc
     2641 accgacttct tcaattgcta cggcggcgcg agcaatccgc tcacgctggc ggccaatgtc
     2701 acgatcaacg cgtccggtgg attgctgcag ggactgggtg gtccggccag tgggttcgtc
     2761 ataaagtcag cgatcaacat gtcgtcggcg gctgggaatc tttcaatcag ctctttcacc
     2821 aatcagggca cgttgaccga cagcgcgggc accgtcacgc tcggcggccc gtgggccaac
     2881 agcggcacca tcgtcgtcaa caacgcggcg gccacgctga acctgggcgg cacgttcagc
     2941 aaggtcggca cgatcaacca cagcgcgggc accgtgaacc tcatgggcac cggcaccatc
     3001 aacggcacgc tcgccttcac cgcggcgacc ggcagctgga agatggccgg cggcacgctc
     3061 gtcaacgcga cgctcagctt cgccggtggc gccagcctgg tgctcaccag cagcggcggc
     3121 acgttcaatc acgtgacgct tgggtcggac ctgaccttcg gcaacaacgg gcagatcacc
     3181 gtagtgaagg ggttgctgct gaacagtcac acgatcacgc tggccagcac cgcggcgtat
     3241 accgggatca tcttcaacga ctcggcagcg cagagcctgt ctggccccgg tcaggtcatc
     3301 ttcgcgggca cttcgcccaa cacagatttc ttcaactgct acggcgcggc cgccattccg
     3361 gtgacgctgg gttccggcgt gacgatcagc gcggccggtg ggttgattca gaatctcggc
     3421 ggcgccaccg cggggctcat taatgcggcg gcaatcaccg tcacgacggg cacgctgacc
     3481 ttgaacggca gcttgaccaa caagggcacg ctctttgcca agggcgggaa catcgtggac
     3541 aacggcgcag aactggtgaa ctccggcacg atcctggtcg ccacgggcca ttcgctgacg
     3601 atggcggtgc cgactgccac gctcaccaac agcggaggtg gcgtgttgag cggcaccggc
     3661 accatcgtcg cgccgaccgt tatcaactcc gccacggtct cgcccggcgc gccgctcacc
     3721 cccggcaccg cggcggctgg gaagctcacc atcaccggca acttcaccca aacggccagc
     3781 ggcacgctgc gcgcggaact cggcggcacc gccggtgggg cgttcgatgt actcgcggtc
     3841 agcggcaccg cagcgctcgc gggtctgctc gacgtctaca ccttcaacgg cttccacccc
     3901 gccaagggca atgtcttcgg cgtgatgact tacaaatcgc acaccggcgc attcaccagc
     3961 accgatggcc tcagcatcgg caacggcgtc gccctcaccg ccgccgccac cggcacgcgc
     4021 gaaacgctca ccggcgtcac catgccagcc gatgccaccg cccccacggt ctccgcgccg
     4081 accctcgtcg gcacgcccgc caagggcggc accaccttgc agttcaagat gaccttcagc
     4141 gacaacatcg ccatcgacat cgccaccctc ggcaacggcg acctcctcgt caccggtccc
     4201 aatggcttca gccaactcgc caccttcgtc agcgtcagca aggccagcaa cggctcgccg
     4261 cgcacggcca tctaccaact cgccgccccc ggcggcacgc tcgatgcgct tgacagcggc
     4321 acctacaccg ccgccgtcca
//
LOCUS       DATIMH010000062         6402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4634043, whole genome shotgun sequence.
ACCESSION   DATIMH010000062 DATIMH010000000
VERSION     DATIMH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4634043"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VLI90_01030"
     CDS             <1..616
                     /locus_tag="VLI90_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HSV12811.1"
                     /translation="AKQGRDRNRQAILPLRGKVLNTEGSSLAKVLENREIADLVIALG
                     CGAGPECDPSKMRYQRVVLLADADSDGHHITTLLLTFIFRHMRALIDAGRVFIALPPL
                     YRVDIGKETYWAADDADKERILKQHAKGNAKSEITRFKGLGEMMQKVLWETTLNPKTR
                     RLLRVEIADALATDRIINELMGKDASARFRFIMERAEEAEELDV"
     gene            1227..1865
                     /locus_tag="VLI90_01035"
     CDS             1227..1865
                     /locus_tag="VLI90_01035"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV12812.1"
                     /translation="MTITHDIVADPPQHIVFDDVSWDFYEHLLREVGNEAIRITYDRG
                     GLEIRSPLPKHERLGEWIGRLLELMCLEREIPIESLGSTTFRSRTKRSGLEPDQCFYI
                     KHAERARSMEEAFDPAVDLPPDLAVEIDITNRSIDREPIYAALRVPELWRFDGKRLQV
                     LHLSAKAKYTRQTRSLSFPFLSLPAFEKYVLRMNDRDQIRTLRDFRKWVAGL"
     gene            1930..2262
                     /locus_tag="VLI90_01040"
     CDS             1930..2262
                     /locus_tag="VLI90_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12813.1"
                     /translation="MNDPAPNHDLPCPGCGYNLRGVPYDHPCPECGLNVQRQPPVGIF
                     DSAKRRATPTVEDFDRQQDELALAAERLLRADEALRRHETLLARWEQLITRIERILDR
                     MEKHPPAE"
     gene            2437..2631
                     /locus_tag="VLI90_01045"
     CDS             2437..2631
                     /locus_tag="VLI90_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12814.1"
                     /translation="MRNSLSRVQFSAFGAAAVLLGVMMLVNRGRAPASASASEMSPGQ
                     TTLSIEKPATQPTAVSDAGR"
     gene            2849..4522
                     /locus_tag="VLI90_01050"
     CDS             2849..4522
                     /locus_tag="VLI90_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV12815.1"
                     /translation="MDTNTNDAALLKAFADTRSHEAFALLVHRHIAMVYAAALRQVRD
                     PALAEDVAQAVFIVLARKAASLGADVILPGWLIRTTHLAARDALKSESRRRRHEQRYA
                     AMATMQTVSTAVADAALAAELSPEIDRALSHLNDTDRGAIVLRYLQDQSVPQVAAALK
                     VSEDAATKRLQRALAKLRGHFARHPMVPSVAMLATALQHLPAPPVVPESLAQATVKTA
                     LAGAPAATTSAAIAKGALEMIFWSQAKVAAASVAIALLAIAVTVGTVSFVRAQNAADI
                     QVKAPTAGVSPATEPASSSQPAPMSNGAVGRLASGVTVELIGVCESPSSGKQWWQADG
                     TLLATAPYWSMTSMGVQMPMAAGTIKREFAARVAKLPVGGNGEPASARWHPMGGDGGA
                     IIAPPRDKAGQVIAGLEARAFILRTSGPIDIRFDFATGPWQMLVESGPDGDRQTHDRA
                     TYEIRKGFTVKDATHFEFVSVNQPLGYTRMIVEDIDGKSQNPNSVRGQWSTSGATQEF
                     AVPLPLARIKRVRVEFRPFDQWITFKNVCVDPKNPTQVKIETSDETQGL"
     gene            4712..6364
                     /locus_tag="VLI90_01055"
     CDS             4712..6364
                     /locus_tag="VLI90_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV12816.1"
                     /translation="MTGDSDRTLLERFTRQRSHDAFAELARRHLDIVYNAALRQVRGD
                     TALAEDVCQAVFIILARKAASLPAGVILPGWLIKTAHLAARDALKTESRRRVHEQRFA
                     AMAPTQTESPASITADLSSEIDRALAHLNGADRGAIVLRFLEGQSIPEVAAALNVTEM
                     TAAKRLQRAVEKLRDYFARHPIMPSVAALTAAMQHLPHAAAPASLAPAATAAAVSGSA
                     TAASSIIARGTMKVIFWTKAKVAAAIAVALLAAAAGTVTLVNAQSTTPAPAPTQSPAP
                     VAGANAPVAEPSSIAHLSNGIWFELVGISDSPSGPHSWWRADGSLLDVAPYQRMAGQG
                     RIISSVPRQVYREIAVRFGAKGDDHAGGPPTISWSVAPSSGSMGSTLVAPRSPDIEAQ
                     KFLLDGSKPVRLRVDVAAGAWRTLFTAGSHGQSGSVGGGPLGGDSVSYTVSTGFEIKG
                     RTHFVLARGVTSNLKIQSDARVLVADNAGHTEVATSAGGSSSANLMSGEYTVAMPLAN
                     IKQLQYQTRPFDQWIEIDNVSVDSSKPTEPKITSSDEPPQGL"
BASE COUNT         1170 a         2093 c         2053 g         1086 t
ORIGIN      
        1 cgcaaagcaa ggtcgagaca gaaatcgtca agccattctt ccccttcgcg gcaaggtgct
       61 caacactgaa ggctcatcgc tggccaaggt gctggagaat cgcgagatcg cggatctggt
      121 gatcgcgctg ggctgcggag cggggccgga atgcgatccg tccaagatgc gctaccagcg
      181 cgtcgtgctc ctggccgacg ccgacagtga cgggcatcac atcaccacgc tgctgctgac
      241 gttcatcttc cggcacatgc gggcgctgat cgacgccggc cgcgtgttca tcgccctgcc
      301 gccgctttat cgggtggaca tcggcaagga aacctattgg gcggccgacg acgccgacaa
      361 ggaacgcatc ctcaagcagc acgccaaagg caacgccaag agcgagatca cccgcttcaa
      421 gggcctgggt gaaatgatgc agaaggtgct gtgggaaacc acgctgaacc ccaagacccg
      481 ccgactgctt cgcgtcgaaa tcgccgacgc gctggcgacc gatcgcatta tcaacgagtt
      541 gatgggcaaa gacgcgtcgg cgaggttccg gttcatcatg gaacgcgcgg aggaggcgga
      601 agagttggat gtgtagcgca gcggtatgtt atggaacccc cgactagcca caccggtggc
      661 gacatcatca cagacttcga cggtgcgctg aaagcatgtg atcgagtgat cggatcggca
      721 cccgagcatt gccaaccgaa catacccact tctcacaagg aactgggagg cgccacgcgc
      781 tggcacgatt ttgaatcgga tgcctgggcg gtgggcgagg cgatccggcg gtggctcgtg
      841 gcaaacccgc caatgccccg cgactctgcc ctcgtggaac gactaatcgc tgtcatcacc
      901 acgcccacgc ttcggcgcgg gcggcagtca ttcttcatgt gcctcggcgc ttctgctcga
      961 gcacatgcag gcgtaatcgc cggcctgctc gcggacgacg acgtctgcgg gcacgcggca
     1021 tccgccctgc tacgaatgaa cgccttgaac ttctctgacc tcatggatcc cctgctgatt
     1081 caccgacacg cgcacgtccg taggacggcc gaacggtaca tcgcccgctg ccgaaacgtg
     1141 tagtatttga acagtgttca aggtggtcac aatttgtgac cggctcgccc ttcgccgtca
     1201 gtccacggca gcgtatcatt tgcacgttga ccatcaccca cgacatcgtc gcggatccgc
     1261 cgcagcacat cgtcttcgac gacgtgtcat gggacttcta cgagcatctt ctgcgcgaag
     1321 tcggcaacga ggcgatccgc atcacctacg accggggagg gctggaaatc aggtcgccgc
     1381 tacccaagca cgagcgattg ggcgagtgga ttggccggct gctcgaattg atgtgcctcg
     1441 agcgcgagat cccaattgaa tctcttggtt cgacgacgtt tcgcagccgg acgaagcgca
     1501 gcggactgga gccggaccag tgcttctaca tcaagcacgc cgagcgcgcg cgaagcatgg
     1561 aagaggcgtt tgatccggcg gtggatcttc cgccggactt ggcggtcgag atcgacatca
     1621 ccaaccggtc gatcgatcgc gagccgatct acgccgcgct tcgtgtcccc gagctgtggc
     1681 ggttcgacgg caagcggctg caggtgctcc atctgtcagc aaaggccaag tacactaggc
     1741 agacgcgaag ccttagcttc ccgtttctgt cgctgccggc gttcgagaag tacgtgcttc
     1801 gcatgaacga ccgcgatcaa atccgcacgc tgcgagattt ccgcaagtgg gttgcgggac
     1861 tttgatggta cgtagcgccg cgacctcgca tgacgaggtg gtcgcaattc gcgaccacct
     1921 ccaaggcgca tgaacgaccc cgcccccaac cacgacctgc cttgccccgg ttgcgggtac
     1981 aacctccgcg gcgtgccgta cgatcaccct tgcccggagt gcgggctgaa cgtgcagcgg
     2041 cagccgccgg tcggcatctt cgactcagcg aaacggcgag caacgccgac agtcgaagat
     2101 ttcgatcgtc aacaggatga actcgcgctg gcggcggagc ggctgctgcg cgccgacgaa
     2161 gccctgcggc ggcacgaaac gctgctggcg cgctgggagc agttgatcac gcgcatcgag
     2221 cgaatcctcg atcgcatgga gaagcacccg ccggcggaat gaaaagcgcg cgccgcgagc
     2281 gcgccttgcc tcctcgtccg ataactgtgc gttcgcccct cattctggcc aacgtcactc
     2341 aatcatcccc tccaatgcgt cgatctgatc aacgtgtccc ttcgcgcgag catgatacgc
     2401 gcatgggcgc gcggtcccga agttgaggag agccaaatgc gaaacagcct gtcccgggtt
     2461 caattcagcg ccttcggcgc cgccgccgtg ttgctcggcg tgatgatgct ggtcaaccgc
     2521 ggccgcgccc ccgcgtcggc gtcggcttcg gagatgtcgc ccggacagac gactctgtcg
     2581 attgagaagc ccgccacgca gccgaccgcc gtcagcgacg ccgggcggta gctgtcggaa
     2641 attcaattgc ccgcccacgc cgggttcgga ggtactccga cgacccggat gctttttgcg
     2701 cgccgcagaa tctcgcttcg tcggagtacc tccgaacccg acgtgattcg ttctcagcgc
     2761 cagcacaatc cgttccaccc cgttatcatc tccgttgtcc accgtctccc ctcggtcgtc
     2821 cgtattcatt gtgaggcgat cactccggat ggatacgaat accaacgacg ccgcgctgct
     2881 gaaggcgttc gccgacacgc gctcgcatga ggcgtttgcg ctgctggtgc accggcatat
     2941 cgcgatggtt tacgccgccg cgttgcggca ggtgcgcgat ccggcgctgg ccgaggatgt
     3001 tgcccaggcc gtgttcatcg tgctggcgcg caaggcggcg tcgctgggcg ctgacgtgat
     3061 cctgccgggg tggttgattc gaacgaccca tctggcggcg cgcgatgcgt tgaagagcga
     3121 gtcgcgccgc cggcgacacg aacaacggta cgccgccatg gccaccatgc aaacagtttc
     3181 gacagcggtt gccgacgcgg ccttggccgc ggagctctcg ccggagatcg atcgcgcgct
     3241 atcgcacttg aacgacaccg atcgcggcgc gatcgttctg cgctaccttc aggaccaatc
     3301 cgtgccgcag gtggcggcgg cgttgaaggt ctccgaagac gcggccacca agcgattgca
     3361 gcgcgcgctg gcaaagctgc gcggtcattt cgcgcgccac ccgatggtgc cgagcgtcgc
     3421 gatgctggcg acggccctgc agcacttgcc ggcgccgccg gtggtaccgg agtcgctggc
     3481 gcaggcgacc gtgaagaccg cgctggccgg cgcgccggcg gcgaccactt ccgccgccat
     3541 tgccaaagga gcgctcgaga tgatcttctg gagccaagcc aaagtcgccg ccgcgagcgt
     3601 tgctatcgcg ctgcttgcta tcgccgtgac cgtcggaacc gtctcgttcg tgcgcgccca
     3661 gaacgccgct gacatccaag tcaaagcacc cacggccggc gtgtcgcccg cgaccgagcc
     3721 cgcctcgtcg tctcaacccg caccgatgtc gaacggcgcc gtcggccgcc ttgccagcgg
     3781 ggtcacggtt gagctaatcg gcgtgtgcga atcgccttcg tccggcaagc aatggtggca
     3841 ggcggatggc acgctcctgg cgaccgcgcc gtactggtcg atgacttcca tgggcgtcca
     3901 gatgccgatg gccgccggca cgatcaagcg cgagttcgcg gcgcgcgttg ccaaactgcc
     3961 ggttggcggc aacggtgaac cggccagcgc gcggtggcac ccaatgggcg gcgacggtgg
     4021 tgccatcatc gcgccgccgc gcgacaaggc ggggcaggtc atcgccggtc tggaagcccg
     4081 cgcgttcatc ctgcggacgt ccgggccgat cgacatccgc ttcgactttg ccaccggccc
     4141 ttggcaaatg ctggtcgagt ccggccccga cggcgaccgc cagacgcatg accgcgcgac
     4201 ctatgaaatc cgcaaggggt tcaccgtgaa ggacgcgacg cacttcgagt tcgtcagcgt
     4261 gaaccagccg ctcggctaca cgcggatgat cgttgaggac attgacggca agtcacagaa
     4321 ccctaattcg gtgcgcgggc agtggagcac gagtggggct acgcaggagt tcgcggtgcc
     4381 gctcccgctg gcccgcatca agcgcgtccg ggtcgaattc cgcccgttcg accagtggat
     4441 cacgttcaaa aacgtctgcg tcgacccaaa gaaccccacc caggtgaaga tcgaaaccag
     4501 cgacgaaacg caggggcttt agcgaaatgg cgcgccggtt ccagcgtggt tgaccccgcg
     4561 tagcggtagc cccgcagggg cacgtgtttc tttctcgcga aaacacgtgc ccctgcgggg
     4621 ctaccgctac gccgggtgaa ccacgccaac aattcttccg cgctttgttg tctcaaatcc
     4681 ccgccgccgg tccgtgttac attagaggca catgaccggc gacagcgacc gaaccctgct
     4741 tgaacgattc acgcgccaac gatcgcacga cgcgttcgcg gagctggcgc ggcggcatct
     4801 cgacatcgtc tacaacgcgg ccctgcggca ggtgcgcggc gacacggcgc tggcggagga
     4861 tgtttgccag gcggtgttca tcattctggc gcgaaaggcc gcgtcgctgc cggcgggggt
     4921 gatcttgccg gggtggttga tcaaaacggc acatctggcc gcgcgcgatg cgctcaagac
     4981 cgaatcgcgg cggcgcgttc atgaacaacg gttcgcagcc atggcaccga cacaaactga
     5041 atctcccgct tcgatcactg ccgatctgtc ctcggagatc gaccgcgcgt tggcgcattt
     5101 gaacggcgcc gatcgaggcg cgatcgtgct gcggttcctc gaaggtcaaa gcattcccga
     5161 agtcgcggcg gcgttgaacg tcacggagat gacggcggcc aagcgcctgc agcgcgcggt
     5221 ggagaagctg cgcgattact tcgcgcggca tccgatcatg ccgagcgtgg ctgcgctgac
     5281 ggctgcgatg cagcacctgc cgcacgcggc agcgccggcg tcgctggcgc cggcggcgac
     5341 cgccgcagcg gtcagtggat cggcaacggc ggcgtcctca atcatcgcac gcggcacgat
     5401 gaaggtgata ttctggacca aggcgaaggt ggcggcggcg atcgcggtcg cactactcgc
     5461 ggcggcggcg gggactgtga cgctggtgaa cgcgcagtca acgacacctg cgcccgcgcc
     5521 gacgcaatca cccgcgcctg ttgctggcgc gaacgcgcca gtggcggagc catcttctat
     5581 cgcgcatttg tccaacggca tatggttcga actggtcggc atcagcgaca gcccctccgg
     5641 cccgcactcg tggtggcggg cagatggttc actgttggac gtcgcgccat atcagcgcat
     5701 ggccggtcag gggcggatca tctcgagcgt gcctcggcag gtgtatcggg aaatcgcggt
     5761 gcggtttggc gccaagggtg acgaccatgc ggggggtcct cccaccattt cctggagcgt
     5821 cgcgcccagt tcgggttcga tgggttcaac gttggtcgcg ccgcgctcgc cggatatcga
     5881 ggcgcagaag tttctgctcg acggcagcaa accggtcagg ctgcgcgtgg atgtcgccgc
     5941 cggcgcctgg cggacgctgt tcaccgccgg ttcacacggt cagagcggca gcgtcggcgg
     6001 tggaccgctc ggcggcgatt ccgtctccta caccgtcagc actgggttcg aaatcaaagg
     6061 ccgcacgcac ttcgtcctcg cacgcggcgt cacgtcgaac ctgaagatcc aatccgacgc
     6121 ccgcgtgctg gtcgccgaca acgccggcca caccgaggtc gccaccagcg ccggcgggtc
     6181 gagcagcgcg aacctgatgt ccggcgaata caccgtcgcg atgccgctgg ccaacatcaa
     6241 gcaactgcaa taccagaccc gcccgttcga ccagtggatc gagatcgaca acgtgagcgt
     6301 cgattcctcg aagccgacag agccgaagat cacgagcagc gatgaaccgc cgcaggggct
     6361 ttagtggcga aatccgaatg gcgaagtccg aatccccgaa tg
//
LOCUS       DATIMH010000063         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_879557, whole genome shotgun sequence.
ACCESSION   DATIMH010000063 DATIMH010000000
VERSION     DATIMH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879557"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VLI90_01060"
     CDS             <1..874
                     /locus_tag="VLI90_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSV12817.1"
                     /translation="LSRPIDTVLCAVLDSDPHVPLNATVARESSHELAAGIDLLAKLT
                     GAQRVWVAADPEQARGWFGALDPLVRADGLRLIPLSGDYPQSHPTLLLHTLLRRRLRP
                     GRLPVEQGVLLCDAPAAVAVGRCALRDEPMLDVPLAVRDHVARRTHLLTVPIGASVAD
                     ICRAIGVPAGEVMIRAGDFLRDQRVPADAIIGGGGELSLQIATREVPENPDPCIRCGW
                     CVESCPTRINPAGLLEASQRNNAALAEHFGLPACIECGICTYVCPSRLPLLASIRKLR
                     ASGVEASNVPFSPA"
     gene            1014..2252
                     /locus_tag="VLI90_01065"
     CDS             1014..2252
                     /locus_tag="VLI90_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HSV12818.1"
                     /translation="MAESSIQSDPTAAVDPIPSAPRAAYAQLAAALGHSGMTVQRYYL
                     LHVLGAIFPLAAGLVMYGWRAALAMIAVLVSAAGAIFVWRRIGLRGGQLRYAHVLWLA
                     LLLAMMLPATLASRFVLDASAHWPILPLAGVMLAIFCWLLGGLGAGRIHPVVTTYLVI
                     VVLYGGINPMTPRQVLQRNEVGRGDLMDVGRAPPALTAQDAWTKRPIVPDHSATAEVP
                     ASQFLSEYTTGRVSPDRVWLSLEGLLRDRLPPLEDLILGATPGPIGTGSAIAIIIGGL
                     LLLYRGLIDFRIPLITVVVAMVALLVLPVPVVITDHPEWRWLAGHVRGIGWSMGVTFV
                     NYEILASPLLFTAFFLATSPSTRPLTRRGRVIYAMLIGMLAAVFQLYVSVAFGPYLAL
                     MTASLLTPTLDKWLRPRALV"
     gene            2287..3021
                     /locus_tag="VLI90_01070"
     CDS             2287..3021
                     /locus_tag="VLI90_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HSV12819.1"
                     /translation="MESLNDHIQSWAAALGPTGEAILRLILAALAGGLIGLEREVRGR
                     QAGFRTNTLVCLGSCLVMLVSVQFGLHAWPTHPPGSGININVDPARIAYGVMTGIGFL
                     GAGTIIKHRTGVLGLTTAAGMWCVAAVGLAVGFGMYTVAAAGALGLLLVLWVLEYVQR
                     ALPRLRYRTVTVRRRWEPNCVTETVQRLKQAELKVVDVSFRRTADLSHVDIDLHIVYS
                     DEQRYFKFERELQRDSAYDLMSMCET"
     gene            3076..3672
                     /locus_tag="VLI90_01075"
     CDS             3076..3672
                     /locus_tag="VLI90_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12820.1"
                     /translation="MEQTLMRAGIGLLSILIVVAIILFVSFSGPGGGYVTTVVTKGQS
                     AQSQAQQLSGRDDNGMTAEQSIMLEENSAGGRLHGLVVKAIMPMGPMAVTYGLVKGDE
                     ITEVGPMRMRDEQNADLMKSLAFEAYQRNWPLVIIRNGQELKLSPNSALTAAHPNYFP
                     APGVTPTGNNPPGAAAPMTPPQLPAPPVQQVPQRIPTH"
     gene            complement(3774..>4133)
                     /locus_tag="VLI90_01080"
     CDS             complement(3774..>4133)
                     /locus_tag="VLI90_01080"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HSV12821.1"
                     /translation="TIGQRKGVGVAFGRPIYVVEIDPQQNRVTLGDREQLLKPGLIAH
                     QINLIGERLRTPTRCTAKIRYNHDPQPAIAELSGPDELRVRFETPQSAITPGQAVVLY
                     DGDVVLGGGWIDAALCE"
BASE COUNT          630 a         1250 c         1453 g          800 t
ORIGIN      
        1 cctgagccgg ccgatcgaca ccgtgctttg cgcggtactg gacagcgacc cgcatgtgcc
       61 gttgaacgcg acggtcgcgc gcgagtcgag ccacgagctg gcggcgggta tcgatctgct
      121 ggcgaagctg accggcgcgc agcgcgtgtg ggtggcggcc gacccggagc aagcgcgcgg
      181 ctggttcggc gcgctggatc cgctcgtgcg cgccgatggc ctgcggctta tcccgctgag
      241 cggagactat ccgcaatcgc atcccacgct gctgctgcac acgctgctgc gccgacggct
      301 gcggccgggg cgactgccgg tggaacaggg cgtgctgctg tgcgacgctc ccgcggccgt
      361 ggccgtggga cggtgcgcgc tgcgcgatga accaatgctg gatgtaccgc tggcggtgcg
      421 cgatcacgtc gcgcgtcgca cgcatttatt gacggtgccg atcggcgcga gcgtcgccga
      481 catttgccgg gcgatcggcg tcccggcggg tgaggtgatg atccgcgcgg gagacttcct
      541 gcgcgatcag cgcgtaccgg cggacgcgat catcggcggt ggtggcgagc tgtcgctgca
      601 gatcgcgacg cgcgaggtgc cggaaaaccc cgacccctgc atccgctgcg gctggtgtgt
      661 cgagtcctgc ccgacgcgca tcaaccccgc gggattgctg gaagcatcgc agcgcaacaa
      721 cgcggcgctg gccgagcact tcggcctgcc cgcgtgcatc gagtgcggca tttgcacgta
      781 cgtctgcccc tcccgtttgc cgctgctggc gtcgatccgt aaattacgcg ccagcggcgt
      841 cgaagcgtcg aacgtcccgt ttagcccggc gtgaacatac gccgggttta tgtggatctt
      901 cgcggacctc tgattgaaaa aaatcaaacg cctcgacttc ttgacgtaac ccggcgtgtg
      961 ttcacgccgg gctaaatgag ggtgctttta cttccgggcg tcgttgcgcg gtgatggcgg
     1021 aatcttccat ccaatccgat ccgaccgccg cggttgaccc gattccttcg gccccgcgcg
     1081 cggcgtatgc gcagctggcg gcggcgctgg ggcacagcgg catgaccgtg cagcgctatt
     1141 acctgctgca cgtcctgggc gcgatcttcc cgctggcggc ggggttggtg atgtacggct
     1201 ggcgcgccgc gctggcgatg atcgcggtgc tggtcagcgc cgccggcgcg atcttcgtct
     1261 ggcggcgcat cgggcttcgc ggcggacagc tgcgatacgc tcacgtgctc tggctggcgc
     1321 tgctgctggc gatgatgctg ccggcgacgc tggcgtcgcg ctttgtgctc gacgcatcgg
     1381 cgcactggcc catcctcccg ctggcggggg tgatgctggc gatcttctgc tggctcctcg
     1441 gggggcttgg tgctgggcgg attcatccgg ttgtcacaac gtacctggtg atcgtcgtgt
     1501 tgtacggtgg gatcaacccg atgacgccgc ggcaggtgtt gcagcggaat gaagtgggac
     1561 gcggtgatct gatggatgtg ggtcgagcgc cgccggcgct taccgcgcag gatgcgtgga
     1621 cgaagcggcc gatcgtgccg gatcacagcg cgaccgccga ggtgccggca tcgcagttct
     1681 tgtcggaata cacgaccggc cgcgtgtcgc cggatcgagt atggttgtcg ctggaaggct
     1741 tgctgcgcga ccggctgccg ccgctggagg atttgatttt gggcgcgacg ccgggaccaa
     1801 tcggcacggg cagcgcgatc gccatcatca tcggcggctt gttgctgctg tatcgcgggc
     1861 tgatcgactt tcgcatcccg ctgatcacgg tggtggtggc gatggtggcg ctgctcgtgc
     1921 tgccggtgcc ggtggtgatc accgatcatc ccgagtggcg ctggctggcc gggcacgtgc
     1981 gcggcatcgg ctggtcgatg ggcgtgacgt tcgtgaacta cgagatcctc gcctcgccgc
     2041 tgctgttcac ggcgttcttc ctggcgacgt cgccgtcaac gcgaccgctg acgcggcggg
     2101 gacgcgtgat ttatgcgatg ctgatcggca tgctggcggc ggtttttcag ctgtacgtgt
     2161 cggtggcgtt cgggccatat ctggcgctga tgacggccag cctgttgacg ccgacactgg
     2221 ataaatggtt gcggccgcgc gcgctggtat gactgacaga accgccgaca ctttaggcgg
     2281 ctacacatgg aaagtttgaa cgatcacatc cagtcatggg cggcggcgct gggtcctacc
     2341 ggcgaggcga tcctgcgtct gatcctggcc gcgctggcgg gcgggctgat cggcctggag
     2401 cgcgaggtcc gtggccggca ggcgggcttt cgcaccaaca cgctcgtctg cctgggcagc
     2461 tgcttggtga tgctcgtgtc ggtccagttc ggcctgcacg cctggccgac gcatccgccg
     2521 ggcagcggga tcaacatcaa cgtcgatccc gcgcgcatcg cttatggcgt gatgaccggc
     2581 atcggatttc tcggcgcggg cacgatcatc aagcatcgca ccggcgtgct gggcctgacc
     2641 accgccgcgg gcatgtggtg cgtggccgct gtgggtttgg cggtcggctt cggcatgtac
     2701 accgtcgccg cggcgggggc gcttgggttg ctgctggtgc tctgggtgct cgagtatgtg
     2761 cagcgcgcgc tgccgcggct gcggtatcga accgtgacgg tccgccggcg gtgggaaccg
     2821 aattgcgtga ccgagacggt gcagcgattg aagcaggcgg agctgaaggt ggtggacgta
     2881 agcttcaggc gaaccgcgga cctgtcgcac gtcgatatcg atctgcacat cgtctattca
     2941 gatgagcagc ggtacttcaa gttcgagcgg gagttgcagc gcgattcggc ttacgacctg
     3001 atgtcgatgt gcgagaccta gtgaaaaagg ttccccgccg aaccgtcatc tgacatagaa
     3061 tgatgtagtc gttttgtgga gcaaaccctc atgcgcgccg gcatcggcct ccttagcatc
     3121 ctgatcgttg tggcgatcat cctgttcgtg tccttctctg gtcccggcgg gggatacgtc
     3181 acgaccgtcg tcaccaaagg gcagagcgcg cagtcgcagg cgcagcagtt gtcgggccgt
     3241 gatgacaacg ggatgaccgc cgagcagtcg atcatgctgg aggagaattc cgccggtggc
     3301 cggctgcacg ggctggtggt gaaggcgatt atgccgatgg ggccgatggc ggtgacctac
     3361 ggcttggtga agggtgacga gatcaccgaa gtcgggccga tgcgcatgcg cgatgagcaa
     3421 aacgccgact tgatgaagtc gttggcgttc gaggcgtatc aacgaaattg gccgctggtg
     3481 attatccgga acggacagga attgaagctc agtccaaaca gtgcgctgac ggcggcgcat
     3541 ccaaattatt tccccgcgcc tggtgtgaca ccgacaggca acaaccctcc gggcgcggcg
     3601 gcgccgatga cgccgccgca attgcccgca ccgccggtgc agcaggtgcc gcagaggatc
     3661 ccgacacatt aatcaggaca gaattgaaac accaagagca cgaagaacac gaagaacagc
     3721 gaactgcttt gcttcgtgct cttggtgagc ttcgtggttc aattccatcg tcttcactcg
     3781 caaagcgcgg cgtcgatcca tccgccgccc aggaccacat ccccgtcata caacaccacc
     3841 gcttgtcccg gtgtgatcgc gctttgcggc gtctcgaacc gcacgcgcag ttcgtcgggg
     3901 ccgcttaact cggcgattgc cggctgcggg tcgtggttgt agcggatctt ggcggtgcac
     3961 cgcgtcggcg tgcgcaggcg ttcgccgatc agattgatct ggtgcgcgat caggccgggc
     4021 ttgagcagct gctcgcggtc gccgagcgtc acgcggtttt gctgggggtc gatctcgacg
     4081 acgtagatcg gccggccgaa cgcgacgccg acgcctttgc gttggccaat cgt
//
LOCUS       DATIMH010000064         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3190223, whole genome shotgun sequence.
ACCESSION   DATIMH010000064 DATIMH010000000
VERSION     DATIMH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3190223"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VLI90_01085"
     CDS             complement(<1..1187)
                     /locus_tag="VLI90_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12822.1"
                     /translation="MSITAVCPSCGKRFQAPAQFAGKRVKCKQCGVVFQIAAQGGGAA
                     APAAGTAGTQRNQTNSQSKAGDQIDIDDSLAQLESVASFAGEDAPPPQASRAGSKVGS
                     KAGAAGGTSAGAGAGGKPRIVGRDELNTKMEANPDAEEVELEGPTDVSYRANVLKFRY
                     PGAKIIDQWLPLFLLVLGFGLMAMVISPRDQTYGIRWIAAARFVIPVLLYRILILPMA
                     MAMVHKAGQEMRYQMPPNEKLRGFATYLPALAFAFTFWTASGGSVLGLILGAFIGLAL
                     SSAALWLLFRLRDEEVSTSIGYGAGGFGIGMGIAIALILGLNLLMTVVLTQTKSQAKV
                     PASPFGPNLSWVSTSSSSTAVANAKPAKAAPKPAAPTDTQASAPTKSNIDSAVLSDVQ
                     TSPV"
     gene            complement(1332..3062)
                     /locus_tag="VLI90_01090"
     CDS             complement(1332..3062)
                     /locus_tag="VLI90_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSV12823.1"
                     /translation="MTGTFLEIKNGEQAHRVPFTKDKFSLGRGPNNDIVLVDDRASRA
                     HAVIERVSGGFYLRDLNSRNGTKLNGERVATVLELNDGDVIEIGRHQISLFVDNQPEV
                     ETIEELTADDLVEADGDVEEASQLDHVLPTEFFADDESVEVQLQKLAANLPHKGFEAG
                     DIELTDSRGQNLRGEGRTSYAQGEAIPLLKQLLLVCFRARATDIHVEPRQDGHYIRLR
                     IDGQMVDVAAVPAKTGVRLSTVVKVLCQIDIGQRTSIQEGAFAAKMPDIKHAGKSHRV
                     DYRVSFAPAVLGQKLVVRILDASGAPARLSDLGFHRHMMETVARVIHQDSGMVLVCGP
                     TGSGKTTTLYSLLRSIDLAKRNVVTIEDPVEVHLDGITQIPVDDEHERSFLQLLRSVL
                     RQDPDVILVGEIRDAETARVAMQAAITGHMVFSTVHTRDTLGTVFRLRDLGVEPYMLG
                     QGLQLVIAQRLVRQLCPFCKQAVAPTPRQLAALGPKNANVAQIFKPVGCPRCLKTGHA
                     GRRAYFELLASNEELAQAIFANATRQDLLKVIQSNPNFTTLRQSALQLVADGFVAFEE
                     VEQDMGVEVQ"
BASE COUNT          613 a         1050 c         1084 g          669 t
ORIGIN      
        1 accggactag tctggacgtc gctgagcacg gccgagtcga tgttggactt cgtcggcgcg
       61 ctcgcttgag tatcggtcgg tgctgccggt ttcggcgcgg ccttggcggg tttcgcattg
      121 gcgacggcgg tcgatgacga cgacgtcgag acccagctca aatttggacc aaatgggctc
      181 gccggcactt tcgcctgcga cttggtctgc gtcagtacga cggtcatgag caggttcaga
      241 ccgaggatca gcgcgatcgc gatgcccatg ccgatgccga aaccaccagc gccgtagccg
      301 atgctggtgg agacctcttc gtcgcgcaag cggaagagca accaaagggc cgcggatgac
      361 agcgccaacc cgatgaacgc gcccaggatg aggccgagca cgctgccacc ggaggcggtc
      421 cagaaggtaa aggcgaacgc gagggcaggc aggtaggtgg cgaagccgcg cagcttttcg
      481 ttcggcggca tctggtagcg catctcctgc ccggccttgt ggaccatggc catggccatc
      541 ggcaggatga ggatccggta aagcaggacg gggatgacga agcgggcggc ggcgatccac
      601 ctgatgccat aggtttggtc gcgcgggctg ataaccatcg ccatcaggcc gaagcccagc
      661 accagcagaa agagcggcag ccactggtca attattttcg cgccgggata gcggaacttc
      721 aaaacgttgg cgcgatagga gacatcggtg gggccttcca gttcaacttc ctcggcgtct
      781 ggattcgcct ccatcttcgt gttgagttca tcgcgtccga cgatgcgcgg cttcccgccc
      841 gcacccgcgc ccgcgctcgt gccaccggcc gcgcccgctt tggacccgac cttcgacccc
      901 gcccgcgagg cctgcggtgg gggggcatct tcaccggcga atgacgcgac ggattccagc
      961 tgagccagcg aatcgtcgat gtcgatctgg tcaccggcct tgctttgcga gttggtctga
     1021 ttgcgctgcg ttcccgcggt gccggcggca ggcgcggccg cgccaccgcc ttgggcggcg
     1081 atttggaaca cgacgccgca ctgcttgcac ttgacgcgct tgcccgcgaa ctgggcaggg
     1141 gcctggaaac gttttccgca ggatggacag accgctgtaa tcgacatcac gagccccttt
     1201 aagccggacc gacttgaaac acaaacaacc gtagccggtt ctgacgcgta cgacgagatt
     1261 tggttcgccc acgagacggg gaatctaacg cgccgcgatc gcgccggata caagacgcgg
     1321 gagcgggcgg attattgcac ctcgacgccc atgtcctgct cgacttcttc gaacgccaca
     1381 aacccgtcgg caaccagttg taacgcgctc tgccgcagcg tcgtgaagtt ggggttgctc
     1441 tggatcacct tcagcaggtc ctgacgggtg gcgttggcga agatcgcctg ggcgagctcc
     1501 tcgttgctcg ccaacaattc aaaatacgcg cggcggccag cgtgcccggt ctttagacac
     1561 cgaggacaac cgacaggttt gaagatttgt gcgacgttgg catttttcgg acccaaggcg
     1621 gccagttgcc gcggggtcgg cgcgaccgcc tgcttgcaga acgggcacaa ctggcggacc
     1681 agccgctgcg cgatgaccag ctgcaggcct tggccgagca tgtacggttc aacgccaaga
     1741 tcgcgcaggc ggaagactgt gcccagcgtg tcgcgcgtgt gcacggtcga gaagaccatg
     1801 tgaccggtga tggccgcctg catcgcgacg cgcgcggtct cggcgtcgcg aatctcgccg
     1861 acgaggatca cgtcgggatc ctgacgcaac acgctgcgca atagttgtag aaacgatcgt
     1921 tcgtgctcgt catcgacggg gatctgcgtg atgccgtcga gatgcacttc gaccggatct
     1981 tcgatcgtga cgacgttgcg cttggcgagg tcgatgctgc gaagcagcga atacagcgtt
     2041 gtcgtcttac cggaacccgt cggcccgcag acgaggacca tgccgctgtc ctggtggatc
     2101 acgcgggcaa cggtttccat catgtggcgg tggaaaccga ggtcgctcag gcgagccggc
     2161 gcgccgctgg cgtcgagaat tcgcacgacc agtttttgcc cgagaacggc cggcgcgaag
     2221 ctgactcggt agtccacgcg gtgcgatttg ccggcgtgct tgatgtccgg catcttcgcg
     2281 gcgaacgcgc cttcctggat agacgtgcgc tgtccgatgt cgatctggca aagcaccttg
     2341 acgacggtcg ataaacgaac gccggtcttc gccgggacgg cggcgacgtc caccatctgc
     2401 ccgtcgatgc gcaggcggat gtaatgccca tcctgccgcg gctcgacgtg gatgtcggtc
     2461 gcccgcgcgc ggaagcagac cagcagcagc tgcttgagca gcgggattgc ttcgccttgg
     2521 gcgtaagaag tgcggccttc gcccctcagg ttctgcccgc gcgagtcggt cagctcaata
     2581 tcgcccgctt cgaaaccctt gtgcggcaga ttcgccgcca gcttctgaag ctgcacctca
     2641 accgactcgt catccgcgaa gaactccgtc ggcaggacgt ggtccagctg gctggcctct
     2701 tcgacgtcgc catcggcttc gaccaagtcg tccgcggtca gctcctcgat cgtctcaact
     2761 tccggctggt tatcaacgaa cagcgagatc tggtgccggc cgatctcgat aacgtcgcca
     2821 tcgttcagct cgagcacggt cgcgacccgc tcgccgttta actttgtgcc atttcggctg
     2881 ttaagatcgc gcaggtaaaa cccgccggac acgcgttcga tcaccgcatg agcccggctg
     2941 gcgcgatcgt ccacgagtac gatgtcgtta ttcggccccc gacccaagct gaacttgtcc
     3001 ttggtgaagg ggacacggtg agcttgctcg ccatttttga tttcgagaaa cgtgcctgtc
     3061 atgttgctac ttctgccgat tgtatcacct ttttccgcag ccttcgaggt ggtttgttga
     3121 ctttcgcaag cgtctttggg gcgaaccgcg ccgcgtgatg ggcgtatcac tccagccgcc
     3181 ctggtggcgg ccgacaccga aaaatgattt ggacggtgtt gacggcgacg aagcgttctg
     3241 gctatacttc gcctgcgtta gttgatcact ccaccggcaa ggtttggtcg cggtgccctt
     3301 aactgcgacg gttagacgaa cctgttagcc gcctgttgcc tcggcaacaa actctttgag
     3361 tgaagaagct gacttcgaac cgcggttgta accgcgaaag ctttttgtcg gttctg
//
LOCUS       DATIMH010000065         4902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1746617, whole genome shotgun sequence.
ACCESSION   DATIMH010000065 DATIMH010000000
VERSION     DATIMH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746617"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..621)
                     /locus_tag="VLI90_01095"
     CDS             complement(136..621)
                     /locus_tag="VLI90_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12824.1"
                     /translation="MRKRELNTYRRLLPGAILVVSLGLSALYAHRAAGKGDGSLLDDD
                     KISLAQLEQKIADSNAPPAVWMSYGRRLYDAKEYSRAALAYAHVMEAEPFNRDARFQR
                     ALALAGAGDAEGLLGYLRDQLYTDPKLPAEALGRSELRQFLNDPRFAALQKDAAAQAM
                     D"
     gene            664..3801
                     /locus_tag="VLI90_01100"
     CDS             664..3801
                     /locus_tag="VLI90_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HSV12825.1"
                     /translation="MNERPVAVKTKKAARASMWLTLACACLLLAGAQLFFAGYSFGVG
                     NQTIQVPLLQRAVNHALYARDAMITTTAADYPTFFFRILAIPAHVIPIRTLYYLLQIV
                     TAAGVFAAVFALARAMFADWLSGAAAALLLVAGHHQALAGDGLYSIGFTHTFAVFPLA
                     IVALALAYRGRYAWAFAMAGVLFNLHALTAAYLLVLLGGAWIADRPRFSRGLLLAFIG
                     IVTAAPTLIVMLSHRQAFDTQWLTLTRVRSADHSFPSAWWQAGSAELPRFALLAGLFA
                     LSLGVRMDRRVRRKSIAMMVAAAALFAAGYVFTDLWPVPLVVRAQLFRCSRLIMVLML
                     LHIAYAAVCAWRGRRPLEIIAAALLALTLALPPLLPMLPIAFAVALVVVLINGRLSIA
                     QSIAAGVVIFICVAARSDLGFSLFAAPSHFMPNWRIGVGILCLALAVASIYIARLPPR
                     PRLAARGVIALFAGALLVAAGAQPPRDGWTWAQLWARDHTPTDAVFLTPVQPGGFRLQ
                     SQRAIVGEWRDGTQLYFSSKFAPMWWDRISALQPGILVDSAGRHILSHGRSMETLDDE
                     ALVDLAHKFGATHIILPRGGRPRGLTPVYYNPDYAIYLPTIQPTEAPADAPDADRWQA
                     DDRFMRDTVLPNIERNRKGDVHLQLLDTDGRPLAGATVDVHQVRQAFDFSASLPFFQA
                     PAGPSTMGDYQPPPVQQAELDRFLQIFNYTMIPFSSKWMYLEPVEGQRHYEELDRYVD
                     FCTQHDIRIEYHFLSGYWPAWLSRKTPKQQGEDFLRHAKQLAERYGNKIDAWQVTNEG
                     ILFQQSPPVFAALRKMLPNAKLGIADCAKFAPESRLAATPALRERDMYRGLDQIRWLK
                     QQGVKVDFFGFHGHRPFGLWPEAKVMYEMFDTFAKEGVRIHVTEVTIPQDANITGPLR
                     SGRLNAAAQAEYYTRFFTICFSHPSVDMVNLWGIGPNTWQRGSGLLNDHYQPKPAFDA
                     LKKLITETWRTNVKLTLGLDGTAGFRGFQGDYEATVNLPSGATSTIKFSIEPAKPATE
                     LRLKLDDRGTFASRD"
     gene            complement(3890..>4902)
                     /locus_tag="VLI90_01105"
     CDS             complement(3890..>4902)
                     /locus_tag="VLI90_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSV12826.1"
                     /translation="VIAELAKASRARGLTFGLSNHRAEHWWYMNGGRAFESDVSDPAY
                     ADFYGPAASMAETPSKAFKDDWLARCCELVDRYQPQLFYFDTWAERPPLKPYLRHFAA
                     YYYNRAASWPSGAGGGVAINYKNDMFAPHTGVIDVERGQLSEIQPTFWQTDTAVGNKS
                     WCHIKDEEYKTPASLIDALVDIVSKNGTLLLNVGPKADGTIADADRNILEEIGRWLAV
                     NGEAIYGTRPWRVFGEGPTEVPDGHFTETKRPAFTAEDIRFTARGQHALYAIALGHPR
                     GEIVIRSLGSHLKLLPQAITDVRLLGFDGALKWSRDAQGLRVQLPAALPSGYGAALQI
                     IY"
BASE COUNT          812 a         1648 c         1526 g          916 t
ORIGIN      
        1 cgttcacaca tctggcgctc atcagcgccg cgtacaacct cgacgcaaca atgggatctc
       61 gaaattggta gcgcgggcgc cagagcccgt gccgaggacc gtcaggcacg ggcgagacgc
      121 ccgtgctacg gcattctaat ccatcgcctg cgccgccgcg tctttctgca gcgccgcgaa
      181 ccgcgggtcg ttcaaaaact gccgcagctc cgatcgtccc agcgcctcgg ccggcagctt
      241 cgggtctgtg tacagttgat cgcgcaagta acccagcaac ccctcggcat cgcccgcgcc
      301 ggccagcgcc agcgctcgtt gaaagcgcgc gtcgcgattg aacggctcgg cctccatcac
      361 gtgcgcatac gccaacgccg cccgcgaata ttccttcgcg tcatacaacc gccggccgta
      421 cgacatccac accgccggtg gcgcgttgct gtcggcgatc ttctgctcca gttgagccag
      481 gctgatcttg tcatcgtcca gcaaagatcc atcgcctttg cccgccgccc ggtgcgcgta
      541 caatgccgac agccccagcg acacaacgag aatcgccccc ggcagtaatc ttctataggt
      601 gttcaactcg cgcttcctca tgctcttgaa tcggccacag ggtctgcgtt gatgatgtta
      661 ctgttgaacg aacgacccgt ggccgtcaaa acaaaaaaag ctgctcgtgc gagcatgtgg
      721 ttgacgctgg cgtgcgcctg cctgctgctc gccggcgcac agctgttctt cgccggctat
      781 tcgttcggcg tcgggaacca gaccatccag gtgcccctgc tgcaacgcgc cgtcaatcat
      841 gcgctgtacg cgcgcgacgc gatgatcacc accaccgcgg cggattatcc cacgttcttc
      901 ttccgcatcc tggcgatccc cgcccacgtc atcccgattc gcacgctgta ttacctcctg
      961 cagatcgtaa ccgccgccgg cgtgttcgcc gcggtgttcg cgctggcgcg ggcgatgttc
     1021 gccgattggc tcagcggcgc cgcggcggca ctgctgctcg tcgcgggaca tcaccaggcg
     1081 ctggccggtg atgggcttta ctcgatcggc ttcacgcaca ccttcgccgt tttcccgctg
     1141 gcgatcgtcg cgctagcgct ggcgtaccgc gggcgctacg cttgggcgtt cgcgatggcc
     1201 ggcgtgctct tcaacctgca cgcgctgacg gcggcatatt tgctcgtcct gctcggcggt
     1261 gcgtggatcg ccgatcggcc tcggttctcg cgcgggcttc ttctcgcgtt catcggcatc
     1321 gtgacggcag cgccgacatt gatcgtgatg ctctcgcatc gccaggcgtt cgacacccag
     1381 tggctcacgt tgacgcgcgt tcgatccgcc gaccacagtt tccccagcgc ctggtggcag
     1441 gccggatcgg cagaactgcc tcgcttcgca ttgctcgcgg gcctgttcgc gctgtcgctc
     1501 ggcgttcgga tggacaggcg cgttcggcgg aaatcgatcg cgatgatggt cgccgccgcc
     1561 gcgctcttcg ccgccggcta cgtcttcacg gacctttggc cggtgccgct ggtcgtgcgg
     1621 gcgcaattgt tccgctgctc gcggctgatc atggtgctga tgctgctgca cattgcgtac
     1681 gccgcggtgt gcgcgtggcg cgggcggcga ccgctggaga tcatcgcggc cgcgctgttg
     1741 gcgctgacgc tcgccctgcc gccgctgctg ccgatgttgc cgatcgcgtt tgccgttgcg
     1801 ctggtcgttg tgttgatcaa cggtcgattg tcgattgccc aatcgatcgc cgcgggcgtg
     1861 gtcatcttca tctgcgtcgc ggcgagaagc gatttaggct tctcgctctt cgctgccccc
     1921 agccacttca tgcccaactg gcgcatcggc gtcggcatcc tttgcctcgc gctcgccgtg
     1981 gccagcatat acatcgcgcg actaccgccg cggccgcgac tggccgctcg cggcgtgatt
     2041 gcgctcttcg ccggcgcatt gctcgtcgcc gctggcgcgc agccgccgcg cgacggatgg
     2101 acctgggcac agctctgggc gcgcgatcac acgccgaccg acgccgtctt tctcacgccc
     2161 gtgcagcctg gcggattccg cctgcagtcg cagcgtgcga tcgtcggcga atggcgcgac
     2221 ggcactcagc tgtacttctc gtcgaagttc gcgccgatgt ggtgggatcg catcagcgcg
     2281 ctgcagccgg gcatcctggt ggattcggcg ggcaggcaca tcctctcgca cgggcgatcg
     2341 atggagacgc tcgacgatga agcgctggtg gacctcgcgc ataaattcgg agccacgcac
     2401 attatcctgc cgcgcggcgg gcggccgcgc ggcctgacgc cggtgtatta caaccccgac
     2461 tacgcgattt acctcccgac gattcaaccc accgaagccc ccgccgatgc gccggacgcg
     2521 gaccgctggc aggcggatga tcggttcatg cgcgacacgg tgctgcccaa catcgaacgc
     2581 aatcgcaagg gcgacgttca cctgcagttg ctcgacaccg acggccgccc gctcgccggg
     2641 gcgacggtcg atgtccatca ggtgcggcag gcgttcgact tcagcgcgtc tctaccgttc
     2701 ttccaagccc cggccgggcc gtccacgatg ggcgactatc aaccgccgcc cgtgcagcag
     2761 gcggagcttg atcgcttcct gcagatcttc aactacacga tgatcccgtt cagcagtaag
     2821 tggatgtacc tcgaaccagt cgagggccag cggcattacg aagagctcga ccgctacgtc
     2881 gacttctgca cgcagcatga catccgcatc gagtaccact tcctcagcgg ctattggccg
     2941 gcatggctgt cgcggaaaac gccgaagcag caaggcgagg atttcctacg tcacgccaag
     3001 cagctggccg agcgctatgg caataagatc gacgcgtggc aggtgaccaa cgaaggcatc
     3061 ctgtttcagc aatcgccacc ggtgttcgcg gcactgcgga agatgttgcc gaacgcgaag
     3121 cttggcatcg ccgactgcgc gaaattcgcg ccggagagcc ggctggctgc cacaccggcg
     3181 ctgcgcgagc gcgacatgta ccgcgggctc gatcagatcc gctggctcaa gcagcaaggt
     3241 gtgaaggtgg acttcttcgg tttccacggc caccgcccgt tcggcctttg gccagaagcc
     3301 aaagtcatgt acgagatgtt cgacacgttc gccaaggaag gcgtgcgcat ccacgtcacc
     3361 gaggtgacga tcccgcagga tgcgaacatc accggcccgc tgcgcagcgg ccgattgaac
     3421 gcggcagcac aggcggaata ctacacgcga ttcttcacga tctgcttcag ccatccatcg
     3481 gtggacatgg tgaacctgtg gggcatcggt cccaacacct ggcaacgcgg cagcgggctg
     3541 ctgaacgatc actaccaacc caagcccgcg ttcgatgcgc tgaagaagct gatcaccgaa
     3601 acctggcgaa cgaacgtgaa gctgacgctg gggctcgacg gcaccgcggg ctttcgtgga
     3661 ttccaaggcg attacgaggc gacggtgaac ctgccatcgg gcgcgacgag cacgatcaag
     3721 ttcagcattg aaccggcaaa gccagcaacg gagttgcgat tgaagctgga tgatcgcggg
     3781 acgttcgcgt cgcgcgattg aggcaattta cgatcaattg gtgcaggctt atcccaaagc
     3841 ggcaagcggc ccgcgcgtgc gaacttcgaa cctgggcacc cgttcgagct caatagatga
     3901 tctgcagcgc agccccgtag ccggacggca gcgccgccgg cagctgcacg cgcaggcctt
     3961 gcgcatcgcg cgaccacttc agcgccccgt caaacccgag caggcgaacg tccgtgatcg
     4021 cctgcgggag cagcttcaga tggctgccga gcgagcggat gacgatttcg ccgcgcgggt
     4081 ggcccagcgc gatcgcgtac agcgcgtgct gaccgcgcgc cgtaaagcgg atgtcttcgg
     4141 cggtgaacgc ggggcgcttg gtctccgtga aatggccgtc cggcacttcc gtcgggcctt
     4201 cgccgaagac tcgccacggg cgggtgccgt agatcgcttc accgttgacg gcaagccagc
     4261 ggccgatttc ttcaaggatg tttcgatcgg catcggcgat cgtcccgtcg gccttgggcc
     4321 cgacgttcag cagcagcgtg ccgttcttgc tgacgatgtc caccagcgcg tcgatcagcg
     4381 acgccggcgt cttgtattcc tcgtccttga tgtggcacca cgacttgttc cccaccgcgg
     4441 tgtcggtttg ccagaaggtc ggttggatct cgctgagctg cccgcgctcg acgtcgatga
     4501 cgccggtgtg cggggcgaac atgtcgttct tgtagttgat ggcgacccca ccgcccgcgc
     4561 cgctgggcca gcttgcagcg cgattgtagt agtacgcagc gaaatgccgc aggtacggct
     4621 tgagcggcgg gcgctcggcc caggtatcga agtaaaacag ctgcggctga tatctgtcca
     4681 ccagctcgca gcagcgggcg agccaatcgt ccttgaacgc cttactcggc gtttccgcca
     4741 tcgacgccgc gggaccgtaa aagtcggcgt acgcggggtc gctgacgtcg gactcgaacg
     4801 cccggccgcc gttcatgtac caccagtgct cagcacgatg gttcgacagg ccaaacgtga
     4861 gtccacgcgc gcggctcgcc ttcgccagct cagcaatcac gt
//
LOCUS       DATIMH010000066         5661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3767782, whole genome shotgun sequence.
ACCESSION   DATIMH010000066 DATIMH010000000
VERSION     DATIMH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3767782"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VLI90_01110"
     CDS             <1..1066
                     /locus_tag="VLI90_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12827.1"
                     /translation="DGSHVEVAPPLASPDPGSTLMLIDADGNAANEATVILGAATACT
                     LDGHVPTTWTSKKPPAGFSCSAGDVRRDGQTAMFLVTQRHGSANASVVATDRMGRSLS
                     GWPQKVGGKSFAAPSLGNVFGDDKLEVIVPDFRRHLLAWTCDGKRFGSTFEEAEGHSY
                     DGGQTGLSPEQLQQEKCTSIFKDDINCAGPAALADLDGDGRAEIIVVDADAATLRAWH
                     GDGSPAFAGGDADGVVAHLSAQGNVFGVSVAGPDDNGAFDFFAGNDWVHRDREGHVNV
                     RQMMDPGPAPGGKIDTTCQDTICDIDGDGRADILIGTADGRVVIFHTGVKYTSRWAQW
                     PMLGANPQRTGCWQAALPSR"
     gene            1220..2101
                     /locus_tag="VLI90_01115"
     CDS             1220..2101
                     /locus_tag="VLI90_01115"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HSV12828.1"
                     /translation="MRITQEQIDHYLLHGFVIIPRFLTPEELSAAHENMLRYVPTAEE
                     LAATPERYSWVYDDPEHLQTEFPFAGEALNDVSTHPELIAFIERVLGTPDVVLSQAAI
                     WAKYAGTGDFEQGLHLDYQGNTLVVPRDDGDFRQVNMILYYSDVTEEMGPTYVVSQAH
                     TRELPLWPTHRTRKRSPELYRHERPVLANPGDLFLFGMRTWHRASNITDDFGVRLSHH
                     MVYRAARHGFQGYHLWSRQGENEQLQHFMQRATPRQREVLGFPKPGDSYWNEETRAAV
                     KLRYPGMDMTPYGTDRK"
     gene            complement(2080..2802)
                     /locus_tag="VLI90_01120"
     CDS             complement(2080..2802)
                     /locus_tag="VLI90_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HSV12829.1"
                     /translation="MAAACIGLARSGQLAAVRGPTDGRRFDHRFANVILGVWIVIFLR
                     DLRPDRLAWINSLPLHICDIVGFAAVLAIRYRLHWARAMLYFWGLSLCSQAFCFPVLR
                     AGPIHFDFWLYWLQHGIIVAAAVYDLVVRGYRSSWRDWRRMSIALAIYAVAIVPLNVK
                     LQANYGYLGLVDQAQRSTVAAMGPWPDRLPVMYLAACALMAAMALPWRWKRRGEPAPH
                     AVRAQPADYLWLDAPAYLRSVP"
     gene            complement(3161..4978)
                     /gene="pepF"
                     /locus_tag="VLI90_01125"
     CDS             complement(3161..4978)
                     /gene="pepF"
                     /locus_tag="VLI90_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HSV12830.1"
                     /translation="MMTKTKAKPLPKRSKVKPQDTWDLSSLFKSDAAWEAAFAKWERQ
                     IPGYEKFRGKLGESAEMLSACLQFDASLDRLAERVGVYAYLKTTEDTANSDYQRMKGR
                     YQAMATKAAEAASFIRPEIMSVAAETMDRFIAAREMAEWRLAMERILRYRPHTLDNKG
                     EQLLAMQGRMSEASNEFFRQLNDADLKWPMMRDERGQQIELGHSSFSKFLHSPKRSVR
                     KEAFQKYYGQYEAHKNSMAAALNGSVQRDAYYAKARGYPSALESSLFHDNVPVRVYDS
                     LIDSVHRHLPALYRYYDVRRRAMKLKDGIHQYDTYVPILSDLDKRTTWDQAVKTVIAS
                     LEPLGAEYTRELHRGLTTGRWCDKYPNAGKQSGAFSYGTFDAQPYILMNYQPDVLDHV
                     FTLAHEAGHSMHSYYSAKSQPYQYYSYTIFVAEVASTFNEQLLARHLMDKAKDERERA
                     YLINRQIDGIRGTIIRQTMFAEFERITHALVEADEPLTVDTLKREYRKLLDLYFGPDF
                     VIDSELELECLRIPHFYRAFYVYKYATGLSAAIALAERVTNGGKRELNDYLTFLKGGS
                     SKWPLDLLRDAGVDMEQPAAVDSALGYFERLVGELDGLL"
     gene            complement(5361..5591)
                     /locus_tag="VLI90_01130"
     CDS             complement(5361..5591)
                     /locus_tag="VLI90_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12831.1"
                     /translation="MPTAWKQTSTVGEGGRIEVVVPELQPGDIVEVQVARTMSPSSET
                     GAPVKREMGFLRGEIWIADGFDAPLEDFAEYM"
BASE COUNT         1084 a         1776 c         1739 g         1062 t
ORIGIN      
        1 cgacggcagt cacgtggagg tggcgccgcc gctggcatcg cccgatccgg gcagcacgct
       61 gatgctgatc gatgccgatg gcaacgccgc caatgaggcg actgtgattc tcggcgcggc
      121 caccgcgtgc acgctggatg gccacgtacc gaccacctgg accagcaaga aaccgcccgc
      181 gggtttttcg tgtagcgctg gggacgtcag gcgtgacggg caaaccgcga tgttcctggt
      241 cacgcagcgg cacggcagcg cgaacgccag cgtggtggcg acggaccgga tgggccggtc
      301 gctttcggga tggccgcaga aggtgggcgg caaatcgttc gccgccccgt cgctgggcaa
      361 cgtgtttggc gatgacaagc tcgaagtgat cgtgccggat tttcgcaggc acctgctggc
      421 gtggacgtgc gatggcaagc gcttcggcag cacgttcgaa gaagcggaag gccacagcta
      481 tgacggcggg cagaccggcc tgtcgcccga gcagctgcag caggagaagt gcacgagcat
      541 cttcaaggac gacatcaact gcgccggccc ggcggcgctg gccgacctgg acggcgatgg
      601 gcgggccgag atcatcgtcg tcgatgccga cgccgccacg ctgcgcgcgt ggcacggcga
      661 cggcagcccc gcatttgccg gcggtgacgc cgacggcgtc gtggcgcacc taagcgccca
      721 gggcaacgtg ttcggcgtga gcgtcgccgg ccccgacgat aatggcgctt tcgatttctt
      781 cgccggcaac gactgggtgc atcgcgatcg tgaaggacac gtgaacgtgc gccagatgat
      841 ggacccgggt ccggcgcccg gtggaaagat tgatacgacc tgccaggaca cgatttgcga
      901 catcgacggc gacggccgag cggacatcct gatcggaacc gccgacggac gagtcgtcat
      961 ctttcacacc ggcgtgaagt acacatcgcg ctgggcgcag tggccgatgc tcggggcgaa
     1021 tccgcaacgc accggatgct ggcaggcggc gttgccgagc cgctgagaat tactgtccga
     1081 gcttcgcacc cactgtgacg cgaggcttcg gcccgtgtca cggtagcggt gcgaccgctc
     1141 ctaagggcgc ctcgcttcgt tgcgatggtc ctgacgctca cgcgttgatt tccattgcgc
     1201 gttcctcaaa attgacggcg tgcgaatcac gcaagaacag atcgaccact acctgcttca
     1261 cggctttgtc atcatcccgc gcttcctcac gcccgaggaa ctgtcggcgg cgcatgagaa
     1321 catgctgcgt tacgtgccga cggccgagga actcgcggcg acgccggagc gttacagctg
     1381 ggtgtacgat gatcccgagc atctgcagac ggagttcccg ttcgccggcg aagcgctgaa
     1441 cgatgtctca acgcatccgg agttgatcgc gttcatcgag cgcgtgctgg ggacgccgga
     1501 tgttgtgctc agtcaagccg cgatttgggc gaaatatgcc ggcaccggcg atttcgagca
     1561 ggggcttcac ctggattacc agggcaacac gctggtggtg ccgcgggatg acggcgattt
     1621 tcgccaggtg aacatgatcc tgtactacag cgacgtgacg gaggagatgg ggccgacgta
     1681 cgtcgtgtcg caggcgcata cgcgcgagct gccgctgtgg ccgacgcatc gcacgcgcaa
     1741 gcgcagcccc gagttgtacc gccacgagcg gccggtgctg gcgaatcccg gcgacctgtt
     1801 tctctttggt atgcgcacgt ggcatcgcgc cagcaacatc accgacgact tcggcgtgcg
     1861 cctgagccat cacatggtct atcgcgccgc gcgacatggc ttccagggct atcacctgtg
     1921 gtcgcgacag ggcgagaacg agcagctgca gcatttcatg caacgcgcca caccgcggca
     1981 gcgcgaggtg ttgggcttcc ctaagccggg cgattcatat tggaacgaag aaacgcgggc
     2041 agccgtgaag ctgcgatatc cggggatgga tatgacgcct tacggcacgg atcgcaagta
     2101 agcgggggcg tcgagccaga ggtaatctgc cggttgggct cgaacggcgt gaggggctgg
     2161 ttctccgcgc cgtttccacc gccacggaag cgccatcgcc gccatcagcg cgcacgccgc
     2221 gagatacatc accggcaatc gatcgggcca tggccccatc gcggccaccg tcgatcgctg
     2281 cgcctgatcc accaggccga gatatccgta gttcgcttgg agtttcacat tcagcgggac
     2341 gatcgcgacc gcatagatcg ccagcgcgat tgacatgcgc cgccagtcgc gccagctgga
     2401 gcggtagccg cgcacgacca gatcataaac ggcggcggcg acgatgatgc cgtgctgcag
     2461 ccagtacagc cagaagtcga aatggatcgg gccggcgcgc agcactggaa aacaaaacgc
     2521 ttgcgagcag agcgagaggc cccagaagta gagcatcgcg cgcgcccagt gcaggcgata
     2581 ccgaatcgcc agcacggcgg cgaagccgac gatgtcgcag atgtgcagcg gcagggagtt
     2641 gatccacgcg agccggtcgg gccgcagatc gcgaaggaag atgacgatcc agacgccgag
     2701 gatgacgttg gcgaagcgat ggtcgaagcg acggccgtca gtcggcccgc gtaccgcagc
     2761 cagctggccg ctgcgcgcca gcccgatgca agctgcagcc acgccaagaa gaaccgcgac
     2821 atggattgcg gacccggcgt ggaaggcatg caagtggtct gtccagcgca tgatttacaa
     2881 aacgaggctc attgaattca tcggacttgt cgagctggtt gagtagcgtg aggataagca
     2941 aacttttgcg gtcttgacgt ggctgatcat tacaacgatc gcccacacgg cacaggcgtc
     3001 atgacccgaa ttactagagc gtgtttaaca aatgggcaaa tgtttaatag cccaggccgg
     3061 gaggcccggg atcgaactga tagttgcgct atttccaatc caatccctgg ccttccggcc
     3121 tgggctatca cggccgtcat atttctttaa cgcgctcgtc tcatagtaat ccatccaact
     3181 cccccaccag ccgctcgaaa tagcccagcg ccgaatccac cgccgcgggt tgttccatgt
     3241 ctacaccggc gtcgcgcagc aggtccagcg gccacttgga gctgccgccc tttaggaacg
     3301 tcaggtagtc gttcagctca cgcttgccgc cgttggtgac gcgctcggcg agtgcaatcg
     3361 cggcgctcag gcccgtcgcg tatttgtaga cgtaaaacgc gcgatagaag tgggggatgc
     3421 gcaggcattc caattccaac tccgaatcga tcacgaagtc cggcccgaag tacaggtcca
     3481 gcagcttgcg atattcgcgc ttcagcgtgt cgaccgtcag cggttcgtcc gcttccacca
     3541 gcgcgtgcgt gatgcgctcg aattcggcga acatcgtctg gcggatgatc gtgccgcgga
     3601 tgccgtcgat ctggcggttg atcagatacg cgcgctcgcg ctcgtctttc gctttgtcca
     3661 tcagatgccg cgccagcagc tgttcgttga acgtgctggc cacctcggcg acgaaaatcg
     3721 tgtagctgta atactgatac ggctgactct tcgctgaata gtaactgtgc atcgaatgcc
     3781 cggcctcgtg cgccagcgtg aagacgtgat ccagcacgtc cggctgataa ttcatgagga
     3841 tgtacggctg ggcatcgaac gtgccgtagc tgaacgcgcc gctttgcttg cccgcgttcg
     3901 gatacttgtc gcaccatcgc cccgtcgtca gcccgcggtg cagctcgcgc gtgtattccg
     3961 cgcccagcgg ttccagcgac gcgatcaccg tcttcaccgc ctggtcccac gtcgtgcgct
     4021 tgtccagatc cgacaaaatc ggcacgtacg tgtcgtactg atggatgcca tctttcaact
     4081 tcatcgcccg ccggcggacg tcgtaatagc gatacaacgc cggcagatgg cgatgcaccg
     4141 aatcgatcag gctgtcgtac acgcgcacgg gaacgttgtc gtgaaagagc gatgactcca
     4201 gcgccgacgg atagccgcgc gccttcgcgt aatacgcatc gcgctgcacg ctgccgttca
     4261 acgcggcggc catgctgttc ttgtgcgctt cgtactgccc gtaatatttc tgaaacgctt
     4321 ccttgcgcac gctgcgcttc ggcgaatgca ggaacttcga aaacgacgaa tgacccagct
     4381 cgatctgctg cccgcgctcg tcgcgcatca tcggccactt caaatccgcg tcgttcaact
     4441 ggcggaagaa ctcattcgac gcctcgctca tccgcccctg catcgccagc agttgctcgc
     4501 ctttgttgtc cagcgtgtgc ggtcgatagc gcaggatgcg ctccatcgcc agccgccatt
     4561 cagccatctc gcgcgccgcg atgaagcgat ccatcgtctc ggcggcaacg ctcataattt
     4621 ccggccggat gaagctcgcc gcctccgccg ccttcgtcgc catcgcctga tagcggccct
     4681 tcatgcgctg gtagtcactg ttagccgtgt cctcggtcgt cttcagatac gcatagacac
     4741 cgacgcgttc cgccagccga tccagcgacg catcgaactg cagacaagcc gacagcatct
     4801 ccgcgctctc gcccagcttc ccgcggaact tttcgtaccc cggaatctgc cgctcccatt
     4861 tcgcaaacgc cgcctcccac gccgcgtcgc tcttgaacag cgacgacaga tcccacgtgt
     4921 cctgcggctt gactttgctg cgcttcggaa gcggcttggc tttggtcttg gtcatcatgt
     4981 tgggttctta ccagatgcgt gtgattccgt atgcgtcaag tgccgcgtcg gcgctgacca
     5041 gcgggaaagc ctcgaccagg ctctgtgcca cgagcatccg gtcgaaaggg tcgcggtggt
     5101 ccgaccgcgg caagatgagg ccgcgtgata ggcaacagct ccaccgaagc tccttgcata
     5161 tgagaactga agagctctgc caatgatggg cgcagccgca gcttttccaa tcgacacttt
     5221 gatcgcgatc tcccaagcac ctgcaacact gagcagtaag cagttggcgc ggtcatccaa
     5281 aacggctttg ccaggcgctg agagttgcga gggatcggta accatccaga gaaaggtgtg
     5341 cgcgtcaata atcgccgtca ttacatgtac tcggcgaaat cttccagagg agcatcgaag
     5401 ccatcggcaa tccagatctc acctcggaga aagcccatct ctcgctttac aggcgcgccg
     5461 gtttcgctgc ttggcgacat ggttcgagcc acctgtactt caacgatgtc acctggctgt
     5521 aattcaggca ccacgacttc gattcgtccg ccttcgccca ctgtgctggt ttgtttccat
     5581 gcggtcggca tatttcgatt ataccacggc cgcgtcgagt ccttcaccct gcatcgcttt
     5641 tgaaatcaac gttcgggcag c
//
LOCUS       DATIMH010000067         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1169998, whole genome shotgun sequence.
ACCESSION   DATIMH010000067 DATIMH010000000
VERSION     DATIMH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169998"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VLI90_01135"
     CDS             <1..485
                     /locus_tag="VLI90_01135"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV12832.1"
                     /translation="GELLVTPAPSGVHQLAVQLLCSALYPYVRAAKLGLASLAPRDVD
                     PEPGRSVQPDVFVVPPDEITRFRGGEPVERLVLAAEVLSPSSARGDRVKKRALYQRHR
                     VSEYWIVDLDARVIERWRPDDERPEVLAAQLEWLPAGATAPFTLDLVAYFAEVFGEDL
                     "
     gene            complement(482..781)
                     /locus_tag="VLI90_01140"
     CDS             complement(482..781)
                     /locus_tag="VLI90_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1279 domain-containing protein"
                     /protein_id="HSV12833.1"
                     /translation="MRKTFKDLLAEYGVVAVVVYFTIFFAVWIGAWAAIQRGVDLRAL
                     AARVGLSPNGFVASLGAWGAAYIFTKLLQPVRIGVTLLLTPLFAKLYERMRRVPR"
     gene            complement(808..>2037)
                     /locus_tag="VLI90_01145"
     CDS             complement(808..>2037)
                     /locus_tag="VLI90_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12834.1"
                     /translation="RIEPSPHDSMMFFVSFDNHRNGDYKPYVFMTHDFGKTFTSIASN
                     LPTGGPDYVHVIREDPVNKNLLFLGTDVGAYVSMNMGQSWQRFMTGLPTVPVHDLRVH
                     PRDHELIAATHGRGIWIADIDALQQINDKVLAEDVHLFAPPTAYQYGDPPVEGQSQGH
                     QVFAAPSPPYGAVLTYRLTTRQPQVRIAILDAAGDTMQVLNGPGAPGINRIAWPFAGK
                     PAPRPPLGAVQKRDSALQMNRITFVLDSLDKAGMNPMMTGMMRQLVRTGDFTPIMAMF
                     QGRGGGSGFGPPPPWNPRPGEQAIATGGRGGGGGAPSAGGPPGAAAPPNAPAAALDPS
                     AFQTVTPLFQIPGRAPSAGGFGLEFLQTLGFGNSAFAAFGGGGPGVATGDYVAVMTVG
                     GKTLRQKVRVERGGATP"
BASE COUNT          378 a          659 c          639 g          361 t
ORIGIN      
        1 acggagagtt gctcgtgacg cccgcgccga gcggcgtcca ccaactagcg gtacagttgc
       61 tctgcagcgc actgtacccc tacgtgcgcg ccgcgaagct ggggcttgcg tcgttggcgc
      121 cgcgtgacgt cgacccagaa cccggccggt ccgtccagcc cgacgttttc gttgttcccc
      181 cggacgaaat cacgcggttt cgtggcggcg aaccggtcga gcgattggtg ctggccgccg
      241 aagtgttgtc gccgagcagt gctcgcggcg atcgcgtaaa gaagcgcgcc ctatatcaac
      301 gccatcgtgt ctcggaatac tggattgtcg atctcgacgc acgcgtcatc gagcgatggc
      361 ggccagacga tgaacgtccc gaagtgctcg ccgcgcagct cgaatggttg ccggctggcg
      421 caaccgcacc gttcacgctc gatctcgtgg cgtacttcgc cgaggtgttc ggcgaagatc
      481 tctagcgtgg aacccgtcgc atgcgctcat acaattttgc gaacaacggc gtcagcagaa
      541 gcgtgacgcc gatccgcacc ggctgcagca acttggtgaa aatgtaggcc gcgccccacg
      601 ccccgagact cgccacgaag ccgttcggcg acaaaccaac gcgcgcggcc aacgcacgca
      661 gatcgacgcc gcgctggatc gccgcccatg cgccgatcca gaccgcgaag aagattgtga
      721 agtagacgac gacggcgact acgccgtatt cggcgagcag gtccttgaac gtctttcgca
      781 tggctcaacc tagacggcgc gtgatgacta gggtgtcgct cctccgcgtt cgacgcgcac
      841 cttttgacgc agcgtctttc caccgacagt catgacggcg acgtaatcgc cggtcgcaac
      901 gccaggaccg ccgccgccga atgccgcgaa cgctgaattg ccgaagccga gcgtctgcag
      961 gaactcgagg ccgaatccgc ccgcgctcgg ggcgcgtccc ggaatctgga acagcggcgt
     1021 gacagtctga aacgctgatg gatccaacgc tgcggccggc gcgttcggcg gagctgcagc
     1081 tcccggcgga cctcccgcgc tcggtgcgcc accgccacca ccccgaccac cagttgcaat
     1141 tgcctgttcg cctgggcgcg gattccatgg tggcggcgga ccgaagccgc ttccaccacc
     1201 gcgaccctga aacatcgcca tgatcggcgt gaaatcgccg gtgcgcacga gctgacgcat
     1261 catcccggtc atcattggat tcattccagc cttgtcgagc gagtcgagca cgaacgtgat
     1321 gcgattcatc tgcaacgccg aatcgcgctt ctgcacggca ccaagcggcg gacgtggcgc
     1381 cggttttccc gcgaacggcc acgcgatgcg gttgatgccc ggcgcgcccg gaccgttcaa
     1441 cacctgcatc gtgtcgcccg ccgcgtcgag aatcgcgatg cgcacctgcg gttgccgggt
     1501 cgtcaatcga tacgtgagca cggcaccgta gggcggactc ggtgcggcga atacttgatg
     1561 cccctgcgac tggccttcga cgggcggatc accgtactga tacgcggtcg gcggtgcgaa
     1621 gaggtgaacg tcttccgcga gaactttgtc gttgatctgt tggagcgcgt caatatcggc
     1681 gatccaaatg ccgcggccat gcgtcgccgc gatcagctcg tggtcacggg gatgcacgcg
     1741 gagatcgtgc accggcacag tcggcagccc ggtcatgaat cgctgccagc tctgacccat
     1801 gttcattgac acgtacgcgc ctacgtcggt gccgaggaag agcaaattct tattgacggg
     1861 atcctctcga atcacgtgga cgtagtcggg accccccgtc gggagattcg acgcgatgga
     1921 tgtgaacgtc ttcccgaagt catgcgtcat gaagacgtac ggcttgtagt cgccgttccg
     1981 gtggttgtcg aacgaaacga agaacatcat cgaatcgtgt ggcgaaggct cgatgcg
//
LOCUS       DATIMH010000068         5032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_592982, whole genome shotgun sequence.
ACCESSION   DATIMH010000068 DATIMH010000000
VERSION     DATIMH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592982"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VLI90_01150"
     CDS             complement(<1..687)
                     /locus_tag="VLI90_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12835.1"
                     /translation="MPLLQATLIGLIALILAPGALFYFDVTPKLVVLLAGTAALCAIG
                     SRHAWNRFSLLLVLSLGSVVLSTAFSPAPALSLYGSTWRRYGLVAQFAVLLFAWFVSQ
                     SPDRRTILRGISIAGAISAIYGIAQYFGWDPILAASAYHIGEGVWTIVRPPGTLGYVS
                     YFATWLLVCGFLSLSLHTRLAYVATALCWTAMLLTGTRAALLGLIVGLLVWLYRRGFR
                     VPRRAIACCGL"
     gene            714..1823
                     /locus_tag="VLI90_01155"
     CDS             714..1823
                     /locus_tag="VLI90_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12836.1"
                     /translation="MAESFDAFRYMSYLRTRWRSIAASCGTAVVLALIVSLLMPRQYT
                     AAARIVIEPPAGTDMRAAVAVSPIYLESLRTYEQFASGDSLFQQAVNHFGLRSGPVES
                     QKRRVLKVGLLRNTRILEIWATLPDPKKAQALAQYLAQATVDMNRGLTADGDQDLVLG
                     MVQQQRDFRQRLAESDAAWVELLSREPVQALQTETENAAELRSNLEQQISNVELEIAD
                     AAERLKQAVGGDAVEIRKQETNARARLAQLRSQMDTLARESRDREKLLAVRLAHRERL
                     EVDRKAVQAQLNAVETQLREARGGASYRGERLKIIDPGIVPERPSSPNVPLNVAAAFL
                     LGLVFPVLWLTLRMGYEDQRAAGRRSGFTKFAKGE"
     gene            1823..3217
                     /locus_tag="VLI90_01160"
     CDS             1823..3217
                     /locus_tag="VLI90_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSV12837.1"
                     /translation="MPGAIQSERLRPYFVGMLFGFWAAAVSLAPSLALKAAFAAPAVV
                     LPVAWWTVQRPHRWIAMFLATALLLPPLPVALGDSGPNACLFFAALGLIAGSLWASDW
                     RIDISGVTAALVALSGVMLASVASAAWYSGWTAAAGSLARVGLFGITVYVYVFAVGQS
                     HSGDTRPSAGLPHGLYWIAVGSAVFACVDFYFQFPAPAGFGPQFVWLDSGVYRRAQGF
                     FYEASTLGNFCAFFLVMIAVAFSRPREQSPISRPALAAGGAIFFAALVLSYSRGSLIN
                     LAIAVAVLMWRNRRHLRIARLAIAAAVCAVVTWRVFPMFAQIYWLRLSATAEYLFSAT
                     EGVLSGRVASWRTLLAWIAEHPWHTIFGIGYKTLPYTDYLGAPIVADNMYLSLLVETG
                     IAGLGALIWLNVAILRAAGRASYSADPRRAFCGTWMLCFWAGEVVQMASGDLLTYWRV
                     LPVYFFVLALAARV"
     gene            3214..4359
                     /locus_tag="VLI90_01165"
     CDS             3214..4359
                     /locus_tag="VLI90_01165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV12838.1"
                     /translation="MKLLLLDQFSDPGGAQQGLLELLPAIRDRGWDALVGLPGDGELF
                     ERVTGAGFPVERIECGPYASGRKSAGDIARFLRGTPRLASQIHAMAAAADVVYVNGPR
                     LLPAASLARLQAPVVFHAHSLVGPGMMRRIAGWALRRMNAPVLANCEFVAAPWRRYAD
                     EVSIVFNGVGPGSGPARVSSSDRLTVACIGRIAPEKGQLEFLDSVERIHKRLPDTRYV
                     ICGAALFGEPGAQSYDQEVRCRAADLPVEFAGWVDDAGAVLAHVDVLLVPSAGHEATT
                     RVILEAYAAGVPVIAFASGGIPEVVDDGVTGCLTCSAEEMAAMTIELLCDRGRRAAMS
                     AAAHECWKRRFTLERYQNDVLAALERARRPVAPDPPLPPKPAHIQAG"
     gene            complement(4247..>5032)
                     /locus_tag="VLI90_01170"
     CDS             complement(4247..>5032)
                     /locus_tag="VLI90_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV12839.1"
                     /translation="AVPEKRRATAYSVFFACMFATGILGNSLGGVLPFVLHSRRTVLL
                     SSAAFAALAVFPALRLNEFPRPPIGGRIYPRSKFLVLYLIPFAVWHLATGTFNPFNNV
                     YFKRLGFADQRIGSVFAVAQLVQVGALLAAPFIIRRLGLLNGIVVMMAAAALGLGALG
                     TQPPGTGAVAAYIAYMSFQWMSEPGLNTLLMNHVAEREHSGASALNYVVAFGAQALAA
                     YAGGAMFSRLGYGPALAGAAGLALLAAVLFRVLLARRFDTALK"
BASE COUNT          895 a         1462 c         1736 g          939 t
ORIGIN      
        1 taatccgcag caggcgatcg cacggcgagg cacgcgaaaa ccgcggcggt agagccagac
       61 aagcagaccc acgatgagac cgagcagtgc ggctcgggtg ccggtcagaa gcatggctgt
      121 ccaacaaagc gcagtcgcta cgtaggcgag ccgggtatgt aacgagaggc ttagaaaacc
      181 acagaccagc agccaggtag cgaagtaact gacgtaaccg agagtgcccg gcgggcggac
      241 gatggtccag acgccctcgc caatgtggta cgccgaggct gccagaatcg ggtcccagcc
      301 gaagtactgc gcgatgccgt aaatggcgct gatcgcgccg gcgattgaaa tgccgcgcag
      361 aatcgtgcgg cgatccggcg actgcgagac gaaccacgca aacaggagca cggcgaattg
      421 cgcaacgagc ccgtagcgtc gccacgtgga accatagagg gagagcgccg gagcagggga
      481 gaacgccgtc gagaggacca ccgagccgag cgagagaacg agcaggaggg aaaagcgatt
      541 ccaggcatgt cgcgagccta tggcgcagag cgcggccgtg ccggcgagga gcacgaccag
      601 cttcggcgtg acatcaaagt aaaataatgc gcccggagcg aggatcagtg cgatcaggcc
      661 aataagagtg gcctggagaa ggggcatatc ggttacacta actcagaaac ttcatggcgg
      721 aatcatttga tgcgtttcgt tacatgagtt acctgcggac gcggtggcgc tcgatcgccg
      781 caagctgcgg cacggcggtg gtccttgctc tgatcgtgag tctactgatg ccccgacagt
      841 acaccgcggc ggcccgcatt gtcattgagc ccccagccgg aaccgacatg cgggcggcgg
      901 tagcggtcag cccgatctac ctcgaatcgc tgcggaccta cgagcagttc gcctccggcg
      961 acagcctgtt tcagcaagcg gtcaatcact tcggcttgcg gtcagggcct gtggaatctc
     1021 aaaagcggcg ggtgctcaag gtcgggttgc tgcggaatac gcggattctc gaaatctggg
     1081 cgacacttcc cgacccgaag aaggcacagg ctttggcgca gtacctggct caggcgacgg
     1141 tcgatatgaa ccgcgggctg accgccgacg gcgaccagga cctggtgctg gggatggtgc
     1201 agcagcagcg tgactttcgc cagcggctcg cggagagtga cgcggcttgg gtcgagctat
     1261 tgtcacgcga gcccgtccag gccctgcaaa ctgaaacgga gaacgcggca gagttgcggt
     1321 cgaacctgga acagcagatt tcgaatgtcg agctcgaaat tgccgatgca gcggaacgtc
     1381 tcaaacaggc ggtcgggggg gatgctgtcg agattcgcaa gcaggagacg aacgcgcggg
     1441 cacgcctcgc gcagttgcgg agccaaatgg acactcttgc gcgggagagc cgggatcggg
     1501 agaagctgct cgcggtacgc ctcgcgcatc gcgagcgact ggaggttgac cgcaaggcgg
     1561 ttcaggctca gttgaatgcg gtcgagacgc agttgcggga agcgagaggc ggcgcgagct
     1621 atcggggtga aaggctcaaa atcatcgatc ctggcatcgt cccggagcgg ccttcgtccc
     1681 ccaacgtgcc gttgaacgtc gcggcggcat tccttttggg gcttgtgttc ccggtgctct
     1741 ggcttacttt gcggatgggg tacgaggatc agcgcgctgc gggtcggcgc agcggcttca
     1801 cgaaattcgc gaagggcgag tgatgcctgg cgcgattcaa tcggagcggc tgcgcccgta
     1861 tttcgtgggg atgctgttcg ggttctgggc ggcggcggtc tcactagcgc cttcgctggc
     1921 actgaaggcg gcattcgccg cgcccgccgt ggtgctgccg gtcgcatggt ggacggtgca
     1981 gcgtccacat cgatggatcg cgatgttcct tgcgacggca ttgctgttgc cgcccctgcc
     2041 agtcgctctc ggggattcgg gcccgaatgc ttgcttgttc tttgcggccc tcggcctgat
     2101 tgccggaagc ttgtgggcca gcgattggcg gatcgacatt tcaggggtga cggcggccct
     2161 cgttgcactg tcaggtgtga tgctggcgag tgtggcgtct gccgcgtggt attccggatg
     2221 gacggccgcg gccgggagtc tcgcgcgggt ggggttgttt gggattacgg tttacgtcta
     2281 cgtcttcgcc gtgggacagt cgcactcggg ggacactcgg ccgtccgcag gcctgcccca
     2341 cggcctctac tggattgccg tcggatcggc ggtgttcgcg tgcgtcgatt tctattttca
     2401 atttccagca ccggcgggtt tcggtccgca gttcgtttgg ctggattcgg gcgtgtatcg
     2461 acgagcgcag ggattctttt atgaggccag cactctcggg aacttctgcg cgttttttct
     2521 ggtcatgatc gcagttgcgt tctcacgacc gcgcgagcag tcgccgatct cccggccggc
     2581 gctcgcggcg gggggcgcga tcttcttcgc ggcgctcgtg ttgtcatact cgcgcggatc
     2641 gctgatcaac ctcgcgatcg ctgtggccgt gctgatgtgg cgcaacaggc gacacctccg
     2701 gattgcgcgg ttggccatcg cggctgctgt gtgcgcagtc gttacctggc gagtgttccc
     2761 aatgttcgcg cagatctact ggctgcgcct ttccgcgacg gcggagtacc tgttctcggc
     2821 gaccgagggc gtgctctccg gtcgtgtggc gagttggcgg acgctgctgg cgtggatcgc
     2881 ggagcatccg tggcacacga tttttggcat cgggtacaag acgctccctt acaccgacta
     2941 tcttggcgcc ccgattgtcg ccgacaacat gtacctgagc ctccttgtgg agaccgggat
     3001 cgcgggcctg ggcgcattga tttggctcaa tgtcgcgatc cttcgggcgg caggccgcgc
     3061 ttcgtacagt gccgacccgc ggcgtgcgtt ctgcggaact tggatgctgt gtttctgggc
     3121 gggtgaggtc gtgcaaatgg cgtccggcga cctgttgacg tactggcgcg tgctgccggt
     3181 ttattttttt gtcctggcgc tcgccgcgcg tgtatgaagc ttctcctgct cgatcagttc
     3241 agcgatcccg gcggcgccca gcagggattg ctcgagttgc tccccgccat tcgagatcgg
     3301 ggctgggatg cgctggtcgg gctgcccggg gatggcgagc tattcgagcg tgtcactggg
     3361 gctggtttcc ccgttgagcg aatcgaatgc ggaccgtacg cgtccggccg gaagtccgcc
     3421 ggcgacatcg cgcgattttt gagagggaca ccgcggctcg ctagccaaat ccatgccatg
     3481 gccgccgccg cggatgtggt ctatgtgaac ggcccgcggc tgctgccggc ggcgtccttg
     3541 gcgcgcctgc aagcgccggt cgtatttcat gcgcatagcc tggtcggccc cgggatgatg
     3601 cgccggatcg cgggatgggc gctgcggagg atgaacgcgc ccgttctggc gaactgcgag
     3661 ttcgtggcag cgccatggcg gaggtatgcg gacgaagtgt cgatcgtctt caatggcgtc
     3721 ggcccggggt cagggcctgc gcgtgtcagt tcatcggacc gcctcacagt agcgtgcatc
     3781 ggacggattg cgcccgaaaa aggccaactg gaatttctcg acagcgtcga acggattcac
     3841 aaacgactgc cggacactcg atacgtcatc tgcggcgcgg ctcttttcgg tgagcctggc
     3901 gctcaatcct atgaccagga ggtgcgctgc cgtgccgccg acttgccggt cgaattcgcc
     3961 ggctgggtag acgacgccgg cgccgtgctg gcgcatgtcg atgtgctact cgtgccatcc
     4021 gcgggacacg aggcgaccac acgcgtgatc ctggaagcgt atgccgcggg ggtgccggtg
     4081 attgcattcg cttccggcgg tattcccgag gtcgtcgacg acggcgtgac gggatgcctc
     4141 acgtgctcgg ctgaggagat ggcagcaatg actatcgagt tgctgtgcga tcgcgggcgg
     4201 cgggccgcga tgtcggctgc ggcccacgag tgctggaagc ggcgctttac tttagagcgg
     4261 tatcaaaacg acgtgctagc agcactcgaa agagcacggc ggccagtagc gccagacccg
     4321 ccgctcccgc caaagccggc ccatatccaa gccggctgaa catcgccccg ccggcgtatg
     4381 ccgcaagcgc ctgggcgccg aacgcgacta catagttcaa agccgaggcg ccgctgtgct
     4441 ctcgctccgc gacatggttc atcagcaacg tgttcaggcc aggctcgctc atccactgga
     4501 aggacatata cgcaatatat gccgcaactg cgcctgtgcc tggcggctga gtgccgagtg
     4561 cgccgagacc gagcgcagcg gctgccatca tgactacgat gccgttgagt agtccgagcc
     4621 tgcggatgat gaatggagca gccaagagcg caccaacctg gaccaactga gcgaccgcga
     4681 agaccgaccc aatccgctgg tcggcgaatc cgaggcgctt gaagtagacg ttattgaacg
     4741 ggttgaacgt cccagtcgcg aggtgccaga ccgcgaaggg gatcaggtaa aggactagaa
     4801 acttgcttct gggatagatt cgtcctccga tcggcgggcg tgggaattcg ttcagccgca
     4861 gcgcagggaa gactgccagt gcggcgaagg cggccgacga cagcaataca gtcctgcggc
     4921 tgtgaaggac aaacggcagg acgccaccaa gagagttgcc gaggatgccc gtcgcgaaca
     4981 tgcaggcgaa gaacaccgag tatgcggtgg ctcgccgctt ttcgggcacg gc
//
LOCUS       DATIMH010000069         8741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2324793, whole genome shotgun sequence.
ACCESSION   DATIMH010000069 DATIMH010000000
VERSION     DATIMH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2324793"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            595..1818
                     /locus_tag="VLI90_01175"
     CDS             595..1818
                     /locus_tag="VLI90_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HSV12840.1"
                     /translation="MENEATKDPTPARPSVPRVGLLIETQVGPGRDILRGVARYVREG
                     GPWALHLEARNQMFQEGWEPAWLNNWEGHGIIARFETSTIVNAVRRAGVPAVDVLGDA
                     KDRHFPLVHVDDHAIARTAALHLLDRGFTQFGFVARANEPWSDKRLAGFSRAVGERGF
                     TCSVLQAGDLDELPEEWDSFIGQTAAWIAAQPKPLGLMLCSDRIGPLVTQACRQAGVA
                     VPEEVAMIGVDNDEPLCAICDPPLTSVDPNHEEVGYQAAALLDKMMAGEPWPTVPTLV
                     APRTVVVRQSSDISAIDDPIVSFALSMIREHACSGLQVRDVAEHAPVSRSVLQRRFRA
                     VLGRSVHDEIVRMQLRKAQELLSETDLPLRTVAEKAGFNHQEYMGAVFKTRLGMTPGQ
                     YRRREQPGKATADAD"
     gene            1896..2945
                     /locus_tag="VLI90_01180"
     CDS             1896..2945
                     /locus_tag="VLI90_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSV12841.1"
                     /translation="MQPFKSIVAAALLCITTRAFPDARADFLKLIDRPRVDLAAQVDP
                     PKTTDGLDQINFSYATDATQRVPGILLKSSKAPARSPVVIALHGTGGAKEQMLPLMRD
                     LVARGFIAVAIDGRYHGHRTKAGKGSAEYDDAIYRTWHDAGPTTRPTVEHPLYYDTVW
                     DISRLIDYLQTRGDIDASRIGAIGISKGGIETYFAAAADPRIAVAVPCIGVQSFDWEI
                     EHDQWQGRVATFQPAFDAAIGEAAVEHADRSFVRSFFDRVTPGIAGEFDGPAMLRLIA
                     PRPLMMINGDIDPHTPLPGVTICIDAATAAYHAAGVEDHFAFSIEAKTAHKVTPESQA
                     AAIEWFVQWLKPDAK"
     gene            complement(3104..5674)
                     /locus_tag="VLI90_01185"
     CDS             complement(3104..5674)
                     /locus_tag="VLI90_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12842.1"
                     /translation="MRIAGTRRVHTSRHALIGKATLIEGLETRILMTVNAWKAGVSGN
                     WNDPAKWSLGHFPTTAEDVTITAAGSYTVTVPTVVTTPYFYCNSIVVGGSASGAQKLA
                     VNAYMIINKAFSVGAGDSIDIGAGRFNASTPAGTNCQIDGTITLNGVSGKTSYLTTGA
                     GNLNGAGSIVFATGGANSLGVNLMTSNGSVLGAGITLRGKGGTIQGTFTNKGTMKLEG
                     GATDLWSISGLTNQGSLTSPGGSNITIYNLVNAAGHSLSVTGGFLALKGTYSNAGTIT
                     ASNTKITYGMTFSAAGTVNRTNSPVYFSGVLNAAGGVLKTGDAAGQWIWTTGKLQNGS
                     YDAGALTALKIDKTAPSGGYGLLDNVTLTGDLAIPGGFYVNAINVLSISAGHKIILNG
                     TTATGRAALYVLSTTLTGGGEVLFNTAGNNLANNLLSGNAWTLGGGVVVHGKAGQIEG
                     LTNSGVILADNPGETIQINSVINNGNIISAPGVIRIQRFTQNAGGALTVGIGGTSPGS
                     GYGQFVFFDAVNAPALNGTFNAVTLSGFAPAVGGAFNVATFAKAPTGAFATKNVDAGS
                     GKAFDLAQTTAAISLKGKNVAGAFAARTAAGAMTITGTAAADTIATKQLLGVLYATMT
                     GKTSVFFDRQIISETVNAGAGADSVSVNGPRAVTLAGGDGNDSLTGGSGADRIDGGEG
                     SDLLSGGGGDDTYVFTAALLAQVDTIAETSGNGSDTLDFSALTTAVTVNLTSDAALAT
                     MANRTVKTQAAGQAANIENAIGGAANDSLTGNAAANRFVGGAGNDVIDGAAGNDLLFG
                     GIGNDKITGGAGLDSMYGEAGNDSLFGADGGAKDLLDGGADADVKGSADAVDTIVSIP
                     "
     gene            6045..6605
                     /locus_tag="VLI90_01190"
     CDS             6045..6605
                     /locus_tag="VLI90_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12843.1"
                     /translation="MTAILALLLCGCSKKNSDAGGSSTSGSSAASASSATGDGTTAIT
                     GQGDADAVKAVSDELANHWLKVPDGWISEYPSQTYIATGKRASAESFYREMKALKFYV
                     NADDLSPSDKLNGVQFDGTCQFDPAPTRVFGDPNAFGPKRWSDWHDSSESVRVQKRNG
                     QWTFASNLGYLLEGAKPGADVAAQLK"
     gene            6641..7015
                     /locus_tag="VLI90_01195"
     CDS             6641..7015
                     /locus_tag="VLI90_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12844.1"
                     /translation="MRISLVSRVLLVTIVSIAAVALVGCSKSDSQTSAGGSSSAGAAG
                     DAASKWLGTYQEQGKDHMVLVLQADHKASMGPAGQAPADITWEVAGDDKINVTMGVPM
                     AMFRTSDGGLRDSEGSTWKKTK"
     gene            7523..7711
                     /locus_tag="VLI90_01200"
     CDS             7523..7711
                     /locus_tag="VLI90_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12845.1"
                     /translation="MRAKPFKQDSGPRTREPVSNTYVSEALAKQAAMRPKKTPPREMP
                     VTPKLPRVPKPHRRKRAG"
     gene            7969..>8741
                     /locus_tag="VLI90_01205"
     CDS             7969..>8741
                     /locus_tag="VLI90_01205"
                     /inference="COORDINATES: protein motif:HMM:NF037417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12846.1"
                     /translation="MYRPHLRKVPDRMLLADLRGVARRLGARTMTQAVYRRYGRFAPV
                     TPCIRFGSWNAALAAAGLRASQNYRVEKRAILDDIRAVARRLRAERLAFGAYRALGRY
                     GMGPIYRQFGSWRAAAEAAGVVPRRYAARGDRALYDNLEQVWRRLRRQPRAVDLRHPI
                     SKINLTAYIRRFGTFQAALAAFVRRMRVRGYFNRSIGCPATSIPPPIEIQHKTKRHLS
                     WRLRYEILHRDDFRCRACGCSPATHANVVLQVDHIKPWSR"
BASE COUNT         1575 a         2939 c         2659 g         1568 t
ORIGIN      
        1 cttgcggtgc cgttggcgcc aaaggcactc atcgagcaca tcgtgacagc aaccggcgca
       61 acggcagcca ataccagacg cttccgattc gatcgattca tttgtcccgc tccgaaaaga
      121 gatgtagatc cgcctgccgc gaaaaccgac acctgcgcca ccgaagggcg atccttcagt
      181 ctgacccgca gatcgtctgc gacccctcgc tgtcctgcag acgccccacc cgtctcacca
      241 atcgctgttt tggtagagga atcggttcgc gaccaatatc ataatgtgaa actgccgcag
      301 agcaagaaga tttcgctaat tttgcgatag caaacgatat ctcacattgc acctcaccgc
      361 tttttatttc caatgcccgc cggaaattga tatgctccat ctcgttcgag tccaacggaa
      421 tgtagtaaat gaaccggcgt ctaactccac ggcggagccc ctgatatggc tggtgaagct
      481 gaacttccaa ccaaacccgt cgaccgaaaa gcccgcccgc tgatgttgac aactggggca
      541 atggcaaggg cttggtttgt acgccggggt gggcggatgt taggatcgcc cgcggtggaa
      601 aatgaagcga caaaagaccc gactcccgct cgtccttcgg tcccgcgcgt cggtttgctg
      661 atcgaaactc aggtcggtcc cggccgcgat atcctccgcg gcgtggcgcg ctacgtccgc
      721 gaaggtgggc cttgggcgct gcacctggaa gcgcgcaacc agatgtttca ggaaggctgg
      781 gaaccggcct ggctgaacaa ctgggaaggc cacggcatca tcgcccgctt cgaaacatcg
      841 accatcgtca atgccgtgcg ccgcgccggt gtccccgcgg tggacgtgct gggtgacgcc
      901 aaggatcggc attttcccct cgtacacgtg gacgaccacg ccatcgcccg cacggcggcg
      961 ttgcatttgc tcgatcgcgg gttcacgcag ttcggatttg tcgcccgtgc gaatgaaccg
     1021 tggtcggata agcgactggc gggcttcagc cgcgcggtcg gcgagcgcgg gttcacctgc
     1081 agcgtgttac aggcgggcga tctcgacgag ctgccggaag agtgggattc gttcatcggt
     1141 caaacagccg cgtggatcgc cgctcaaccc aaaccgctgg ggttgatgct gtgcagcgat
     1201 cgcatcggcc cgctggtcac gcaagcgtgt cgccaggccg gtgtcgccgt tcccgaagaa
     1261 gttgcgatga tcggcgtgga caacgacgaa ccgctgtgcg cgatctgcga tcccccgcta
     1321 accagcgtcg atccgaatca cgaggaagtc ggctaccagg ccgccgcgtt gctcgacaag
     1381 atgatggccg gcgagccgtg gccgaccgtg cccacgctgg tcgcgccgcg cacggtggtg
     1441 gtccggcaat catccgacat ctccgccatc gacgacccga tcgtcagctt cgcgctgagc
     1501 atgatccgcg agcacgcctg cagcggactg caggtgcgcg acgtggccga acacgcgccg
     1561 gtgtcgcgca gcgtcttgca gcgccgcttc cgcgcggtgc tcggccgcag cgtgcacgat
     1621 gagatcgtcc gcatgcagct gcgcaaagcg caggagctgc tgagcgagac ggatctcccg
     1681 ctgcgcaccg tcgccgagaa ggcggggttc aatcatcagg aatacatggg cgcggtcttc
     1741 aagactcgcc tgggaatgac ccccggccaa taccgccggc gcgagcaacc cggcaaggcg
     1801 accgccgacg cggattaaaa cgcgcgtcag tagcccccac gccgggttcg gaggtactcc
     1861 gacgacccgg attcttggcg gggatgtcac atcaaatgca gcccttcaag tcgatcgtcg
     1921 cagcggctct gctgtgcatc acgacgcgcg catttcccga cgcgcgagcc gacttcctaa
     1981 aactaataga ccgcccccgc gtcgatctcg ccgctcaagt cgatccgcca aaaactaccg
     2041 acggcctcga tcaaatcaac ttctcctacg ccaccgacgc aacgcagcgc gtccccggca
     2101 tcctgctcaa gtcaagcaaa gcccccgccc gcagccccgt ggtgatcgcg ctccacggca
     2161 ccggcggcgc caaggagcag atgctgccgc tcatgcgcga cctcgtcgcc cgcggcttca
     2221 tcgccgtcgc catcgacggc cgctatcacg gccatcgcac caaggccggc aaaggctccg
     2281 ctgaatacga tgacgcgatc taccgcacct ggcacgacgc cggtcccacc acccggccga
     2341 cggtcgagca cccgctctac tacgacaccg tctgggacat ctcccggctg atcgattacc
     2401 tgcaaacgcg cggcgacatc gatgccagtc gcatcggcgc gatcggcatc tccaagggcg
     2461 gcatcgaaac ttatttcgcc gccgccgccg atccgcgcat cgccgtcgcg gtgccgtgca
     2521 tcggcgtgca gagcttcgac tgggagatcg agcacgacca atggcaaggc cgcgtcgcca
     2581 cgtttcagcc agccttcgac gccgccatcg gcgaggccgc cgtcgaacat gcggatcgat
     2641 cgttcgtgcg atccttcttc gaccgcgtga cgcccggcat cgccggtgaa ttcgacggcc
     2701 ccgcgatgct ccgcctgatc gccccgcgcc cgctgatgat gatcaacggc gacatcgacc
     2761 cgcacacccc gctgcccggc gtcacaatct gcatcgacgc cgccaccgcc gcgtatcacg
     2821 ccgccggcgt ggaagatcat ttcgcgttta gcatcgaagc aaagaccgcg cacaaggtga
     2881 cgccagaatc acaagccgcc gcgattgagt ggttcgtgca gtggttgaaa ccggacgcga
     2941 agtgacgcgc gacgccgatt aacagcccag gccggaaggc cggggatcga tcgacgagct
     3001 tcgccatttc taatccgatc cccggccttc cggcctgggc tgttaatcgg caaggcttcg
     3061 atcgtcgtga atcttaaacg ccgatcgcgc ccgacctcgg caatcacgga atcgacacga
     3121 tcgtgtccac cgcgtccgcc gatcccttga cgtccgcgtc cgcgccgccg tccagcagat
     3181 ccttcgcccc gccatcggcg ccgaacaagc tgtcgttgcc ggcttcgccg tacatggaat
     3241 ccaagccggc tccgccggtg attttgtcgt tcccgatgcc gccgaataac aggtcgttcc
     3301 cggcggcgcc gtcgatgacg tcgttgcccg ccccgccaac gaatcgattc gcggcagcgt
     3361 tgcccgtgag cgagtcgttg gcggcgccgc caatcgcgtt ctcgatgttc gccgcctgcc
     3421 ccgccgcctg cgtcttcacc gtgcgattgg ccatcgtcgc cagcgccgca tcgctggtga
     3481 ggttcaccgt caccgcggtg gtcagcgccg agaaatccag cgtgtccgat ccgttgccgc
     3541 tcgtctcggc aatggtatcc acctgcgcga gcagcgcggc cgtgaacaca tacgtatcgt
     3601 cacccccgcc gccgctcagc aggtcgctgc cttcgccgcc gtcgatccgg tccgccccgc
     3661 tgccgcccgt caggctgtcg ttaccgtcac cgcctgccag cgtcaccgcg cgcgggccat
     3721 tgacggaaac cgaatccgca cccgcgccgg cgttgacggt ttcggaaatg atctgccggt
     3781 cgaagaagac cgatgtcttg ccggtcatcg tcgcatacag caccccgagc agctgcttgg
     3841 tcgcaatcgt gtccgccgcg gcggtgccgg tgatcgtcat cgcccccgcc gccgtgcgcg
     3901 cggcgaatgc gccggcgacg ttcttgcctt tgagcgaaat cgccgccgtc gtctgcgcca
     3961 gatcgaacgc cttcccgctg ccggcgtcca cgttcttcgt cgcgaacgcg ccggtcggcg
     4021 ccttggcgaa ggtcgcgacg ttaaacgccc caccgaccgc cggcgcgaat ccgctcagcg
     4081 tcaccgcgtt gaacgtgccg ttcaacgccg gcgcattgac cgcgtcgaag aacacgaact
     4141 gcccgtaccc actccccggt gacgtgccac cgatgccgac cgtcagcgcc ccgccggcgt
     4201 tctgcgtgaa tcgttggatt cgaatgacgc ccggcgcgga gatgatgttg ccgttgttga
     4261 tgaccgagtt gatttgaatc gtctcgcccg gattgtcggc caggatcacg ccggagttgg
     4321 tcagcccttc gatttgcccc gccttgccgt gcacgacaac cccgccgccc agcgtccacg
     4381 cgttgccgct cagcaggttg ttcgccaggt tgttcccggc ggtattgaac agcacttccc
     4441 cgccgccggt gagcgtggtg ctcaacacat aaagcgccgc ccgccccgtg gcggtggtgc
     4501 cgttcaggat gatcttgtga cccgcgctga tcgaaagcac gttgatggcg ttgacgtaaa
     4561 acccgccggg gatcgccagg tcgcccgtca gcgttacgtt gtcgagcaag ccgtatccac
     4621 cgctgggcgc ggtcttgtcg atcttcaacg ccgtgagcgc gcccgcgtcg tagctgccgt
     4681 tctgcaattt gccggtcgtc cagatccact gccccgcggc atcgcccgtt ttcaaaacac
     4741 cgccggcggc attcagcaca cccgaaaaat agaccggcga attcgtgcga ttcaccgtgc
     4801 ccgcggcgct gaaggtcatg ccgtaggtga tcttcgtatt gctcgcggtg atcgttcccg
     4861 cgttggagta cgtgcccttg agcgccaaaa atcccccggt aacgctcagc gaatgcccgg
     4921 cggcattgac caggttatag atcgtgatgt tcgatccgcc cggcgaggtc agtgaaccct
     4981 gattcgtcaa tccgctgatc gaccagagat cggtcgcgcc gccttcgagt ttcatcgttc
     5041 ccttgttggt gaacgtgccc tgaatcgtgc cgcccttgcc gcgcagggtg atgccggcgc
     5101 cgagcaccga gccgttgctg gtcatcaggt tgacgccgag cgagttggcg ccaccggtgg
     5161 cgaagacgat cgagcccgcg ccgttgaggt tgcccgcgcc ggtggtcaga taagacgtct
     5221 tgcccgaaac gccgttcagc gtgatcgtcc cgtcgatttg gcaattggtc cccgcgggcg
     5281 tgctggcgtt gaatcgaccg gccccgatgt cgatcgaatc acccgccccc acgctgaacg
     5341 ccttgttgat gatcatgtac gcgttcaccg ccagcttctg ggcgccgctc gcgctgccgc
     5401 cgaccacgat cgaattgcaa tagaagtatg gcgtggtgac gacggttggc accgtcaccg
     5461 tgtaactccc ggccgcggtg atcgtcacgt cctcggcggt cgtcgggaaa tgtcccaacg
     5521 accatttcgc cgggtcattc cagtttcccg acaccccggc cttccacgcg ttcaccgtca
     5581 tcaggatgcg cgtttcgagc ccttcgatga gcgtcgcttt gccgatcaaa gcatggcgtg
     5641 acgtatggac acggcgcgtg cctgcgatgc gcatgatatt tcctccgacc cgacagagag
     5701 aagattcgtg ccgcagcgag gtcgactagc gacgcaatcg ccgggatacg aggtgagcga
     5761 gcgctgggca gggtaatcgt ttacagcgca acaatacaag ataaatgcaa caattcccgc
     5821 gggcgggaac ggcggtacga tgacggggcg gcgccgatgt aaagccacga ccagtggtcg
     5881 cggcccgccg ccacgggcgg cggctttaca aggtggctca cggcggtttt tccttgattc
     5941 gcgcgagcgc aacggtctaa tgaacgggca tttcgattca ttgcacgacc gggacggctg
     6001 atatgaacag ggcttacatg ccgttggtta gcaaggccgc gagattgacg gccatcctcg
     6061 cattgctgct ttgcggttgc tcaaaaaaga actccgacgc cggcggcagt tcaaccagcg
     6121 gatcttccgc ggcatccgca tcaagcgcga ccggtgacgg caccaccgcc atcaccggtc
     6181 agggcgacgc cgacgccgtc aaagcggtgt ccgacgagct ggcgaatcac tggctgaagg
     6241 tgcccgacgg ttggatcagc gaatatccgt cgcagaccta catcgccacc ggcaagcgcg
     6301 ccagcgccga gagcttttat cgcgaaatga aggcgctgaa gttttacgtc aacgccgacg
     6361 atctatcacc atccgacaaa ctgaacggcg tgcaattcga cgggacctgc cagttcgatc
     6421 ccgcgccgac gcgcgtgttc ggcgatccca acgcgttcgg ccccaagcgc tggtccgact
     6481 ggcacgacag cagtgaatcc gtccgcgtgc aaaagcgaaa cggccaatgg accttcgcca
     6541 gcaacctggg ttacctgctg gaaggcgcca agcccggggc ggatgtcgcc gcgcagctga
     6601 aatgacattg catggggacc catacaacag gagacgtcca atgcgcattt ccctggtaag
     6661 ccgagttctt ctcgtcacga tcgtttcgat cgccgccgtc gcgctggtcg gctgctcgaa
     6721 atccgattcg caaaccagcg ccggcggcag ctccagcgcc ggcgccgccg gcgatgccgc
     6781 cagcaagtgg ctcggcacct accaagagca gggcaaggac cacatggtcc tggtcctgca
     6841 ggcggaccac aaagcctcga tgggccctgc cggccaggcc cccgccgaca tcacctggga
     6901 agtcgccggc gacgacaaga tcaacgtcac catgggcgtg ccgatggcga tgttccgcac
     6961 ctccgacggc ggcctgcgcg acagcgaggg ctcgacgtgg aagaaaacga agtgagcctc
     7021 caacgcgcta ctttcgaacg gagaattttg ccttattcga tttccgccag ccgcttgcag
     7081 acaggaccgc cctgaagaag gtatatgctt gcgccttata tataggtgag cgcgcttttc
     7141 tcatcccgct cggcatcgct ttggtaatgc cgcacctacc agggcttttt caattcctaa
     7201 cggcaaggca accgccgagc cgccgagagc gccgaggcgg acgccgagag gacgaagtgg
     7261 tggaaccgca gattgcgcgg ctcgattgag ctcaccgaaa tcagatttcg tgagggaaga
     7321 cacagcgttc tcttcaaaat cgcctgctgt attctcggcg gcttacctcg gcgctctcgg
     7381 cgcctcggcg gttgcctttg cgccgatccg gtcgagaacc gaaagacccg cccgcagccc
     7441 gcagccgctc gccatcatcc ggtgggctgt ggtacctttg gcaccgtgac gatccacaac
     7501 attcatccac caggaggctc gcatgcgcgc caaaccattt aagcaggact ccggcccgcg
     7561 cacgcgggag ccggtcagca atacgtacgt cagcgaagcg ctggcgaagc aggccgcgat
     7621 gcggccgaag aagacgcctc cgcgggagat gccggtgacg ccgaagctgc cgcgggtgcc
     7681 caaaccgcac cgccgaaagc gggcggggta gcgtgcttct tcgcgggcct tctgatagtg
     7741 gggcagacac cggggttggg gggatgtgcc atatttttct tcggtcgccg ccgcggcggg
     7801 tcttgcgctt cggtgaggct tcgaaactgt tgtattcccg ctgtatagat agaggcgcag
     7861 ctgttcttcg ctgcccatct cacacacctt tgcatgcgat cgtccgcctg ccgctcgacc
     7921 ccatttcggg gcgcggacgg tgcgcgctcg ccgaaggcga tcgactttgt gtaccggccg
     7981 caccttcgca aggtgccgga tcgcatgtta ctggcggatt tgcggggcgt cgcccggcgg
     8041 ctgggcgcgc ggacgatgac gcaggcggtg taccgccggt atggacggtt cgctccggtc
     8101 acgccgtgca ttcgcttcgg cagttggaac gccgccctgg ccgcggcggg gttgcgggca
     8161 tcgcagaact accgcgttga gaagcgggcg atcctcgatg acatccgtgc cgtcgcgcgc
     8221 cgcttgcgcg ccgagcggct cgcgttcggc gcgtatcggg cgctgggccg atatgggatg
     8281 ggcccgatct accgccagtt cggcagctgg cgagccgccg ccgaggccgc gggggttgta
     8341 ccgcggcgct acgccgcgcg tggggaccgg gcgctgtacg acaatctcga acaggtctgg
     8401 cggcgtctgc gccgccaacc gcgcgcggtc gatctgcgcc atccgatttc gaagatcaac
     8461 ctgaccgcct acatccggcg gttcggcaca ttccaggcgg ccctggccgc gttcgtcagg
     8521 cgcatgcgcg tccgcggcta cttcaataga agtataggat gtcccgccac atcgattccg
     8581 ccacctatag agatacagca caagacgaag cgccacctca gttggcggct gcgctatgag
     8641 atcctgcacc gcgatgactt ccgctgccgc gcctgcgggt gttcgcccgc cactcatgcc
     8701 aacgtggtat tgcaggtcga tcacatcaaa ccctggagcc g
//
LOCUS       DATIMH010000070         2856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5502647, whole genome shotgun sequence.
ACCESSION   DATIMH010000070 DATIMH010000000
VERSION     DATIMH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5502647"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..>2856
                     /locus_tag="VLI90_01210"
     CDS             33..>2856
                     /locus_tag="VLI90_01210"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HSV12847.1"
                     /translation="MRKLLVANRSEIAIRVFRAATELGLETVAIYTYEDRFALHRFKA
                     DESYLIGPPEGGMPVKGYLDVDALIAIAKEHGVDAIHPGYGFLSENAGLARACAENDI
                     TFVGPTPEQLETFGDKTAAKRLAKKTNVPIIPGTEDALTDPAEIKQAAKEIGFPLIIK
                     ASFGGGGRGMRVVRNADELMPRLEECQREAGAAFGRPDVFLERYIARAKHIEVQLLGD
                     GQGNLVHLWERDCSVQRRHQKVVEIAPSVNLPETLRRQICDAAVRLCKPVKYKAAGTV
                     EFLLDLDRNEFYFIEVNPRIQVEHTVTEVVTGIDLVKSQILIAQGHKLHDEPLHVPQQ
                     DQIKTSGYAIQCRITTEDPENQFIPDYGRITTYRSPGGFSIRLDGGNGFGGAVITPYF
                     DSLLVKLTTWGPSFDEAIKRTDRALREFRVRGVKTNIPFLENVILNPTFEKGEATTTF
                     IDTTPELFHFRARRDRATKILTYLGDVIVNSRPDTKGKFNPKKELPDPVIPKFAHGTP
                     PPPGLRNKLLELGPEKFCQWVRKQKGLLITDTTMRDAHQSLLATRFRTYDLVQVADAV
                     AHLTPNLFSLEMWGGATFDTAMRFLQEDPWDRLDQLRARIPNIPFQMLFRGSSALGYT
                     NYPDNVVRELVKKSAEHGMDVFRIFDSLNWTENMKVAMTTVREQTDAICEAAICYTGD
                     ILDPKRTKYSLKYYVKMAKELVQMGTHILGIKDMAGLCRPYAAYALVKALREEIDIPI
                     HFHTHDTSGINAASILRACDAGVDIADAACASMSGMTSQPNLNSIVAALQHTPRDTGL
                     NLESLNRLSDYWEAVREFYYPFEEGMKAGTAEVYEHEMPGGQYTNLRQQAKSLHLEGR
                     WHEIADAYAEVNKMFGDIVKVTPSSKVVGDLALFMVTNNLSPMDILAPKSKLNFPKSV
                     VEMMQGYLGQPEGGWPKVLQKIILDSG"
BASE COUNT          610 a          896 c          865 g          485 t
ORIGIN      
        1 gagccgtggg ctttgtttgt gataggacgt ccatgcgcaa actgctggtt gcgaaccgaa
       61 gtgagatcgc cattcgcgtg ttccgcgccg ccaccgagct gggcctggaa accgtcgcca
      121 tttacaccta cgaagaccgc ttcgcgctgc accggttcaa ggcggatgag tcatacctga
      181 tcggcccgcc tgaaggcggg atgccggtca aggggtatct cgatgtcgat gcgctgatcg
      241 cgatcgccaa ggagcatggc gtcgatgcga ttcaccccgg ttatggattt ctgtcggaga
      301 acgcggggct tgcccgcgcg tgcgccgaaa acgacatcac cttcgtcggc ccgacgccag
      361 agcaactcga aacattcggc gacaagaccg ccgccaagcg cctcgcgaag aagacgaacg
      421 tgccgatcat ccccggcacc gaagacgcgc tgaccgaccc cgcggagatc aaacaagccg
      481 ccaaagagat cggcttcccc ctgatcatca aggccagctt cggcggcggc ggacgcggca
      541 tgcgcgtcgt gcgcaacgcc gatgaattga tgccgcggct cgaagaatgc cagcgcgaag
      601 ccggcgccgc cttcggccgg ccggatgttt ttcttgaacg ctacattgcc cgcgcgaagc
      661 acatcgaagt gcagttgctc ggcgacggcc aaggcaatct ggtccacctg tgggagcgcg
      721 attgctcggt ccagcgacgt caccagaagg tcgtcgagat cgcgcccagc gtgaacctgc
      781 ccgagactct gcgccgccag atctgcgacg ccgcggtgcg gctgtgcaag ccggtgaagt
      841 ataaagccgc cggcaccgtc gaattcctgc tcgatctcga tcgcaacgag ttttacttca
      901 tcgaggtgaa cccgcgcatt caggtcgagc atacggtgac cgaagtcgtc accggcatcg
      961 atctggtcaa gtcgcagatc ctcatcgcgc aaggccacaa actgcacgat gaaccgctgc
     1021 acgtgccaca gcaggaccag atcaaaacca gcggctacgc gatccagtgc cgcatcacga
     1081 cggaagatcc tgagaatcag ttcattcccg actacgggcg gatcaccacg taccgctcgc
     1141 ccggcggttt ttccatccgc ctcgacggcg gcaacgggtt cggtggcgcc gtcatcacgc
     1201 cgtatttcga ttcgctgctg gtcaagctga caacgtgggg gccgagcttc gacgaggcga
     1261 tcaagcgcac cgatcgcgcg ctgcgcgagt tccgcgtccg cggcgtgaag acgaacatcc
     1321 ccttcttgga aaacgtcatc cttaatccga cgttcgagaa gggcgaagcg acgacgacgt
     1381 ttatcgatac gacgccggaa ctgttccact tccgcgcgcg gcgggatcgc gcgacgaaga
     1441 ttctcactta cctcggcgat gtcatcgtca acagccggcc cgacacgaag ggaaagttca
     1501 atccgaagaa ggaactgccc gacccggtga tccccaaatt cgcccacggc acccccccgc
     1561 caccggggct gcggaacaag ctgctggaac tggggccgga gaaattctgc cagtgggtgc
     1621 gcaagcaaaa aggcttgctg atcaccgaca cgacgatgcg cgatgcgcat cagtcgctgc
     1681 tggcgacgcg cttccgcacg tacgatctcg tgcaggtcgc tgacgctgtc gcgcatctca
     1741 cgccgaactt gttcagcctg gaaatgtggg gcggggccac gttcgacacg gcgatgcgct
     1801 tcctgcagga agacccgtgg gaccggctcg atcagctgcg cgcccgcatc cctaacatcc
     1861 cgttccagat gctctttcgc ggctccagcg cgctgggata cacgaactat cccgacaacg
     1921 tcgtgcgcga gctggtgaaa aagtccgccg agcacggcat ggatgtcttt cgcatcttcg
     1981 attcgctgaa ctggaccgag aacatgaagg tggcgatgac gacggtgcgc gagcagaccg
     2041 acgccatctg cgaagccgcc atctgctaca ccggcgatat cctcgatccg aagcgcacga
     2101 agtacagcct gaaatactac gtgaagatgg ccaaggaact ggtgcagatg ggcacgcaca
     2161 tcctgggcat caaggacatg gccggcctct gtcgtcccta cgcggcgtac gcgctggtca
     2221 aagcgctgcg tgaagagatc gacatcccga ttcacttcca cacgcacgat accagcggca
     2281 tcaacgcggc gagcattctt cgcgcgtgtg acgccggggt ggacatcgcc gacgccgcct
     2341 gcgccagcat gagcgggatg accagccagc cgaacttgaa ttcgatcgtc gcggccctgc
     2401 agcacacgcc gcgcgacacg ggtttgaacc tggaatcgct caatcggctg agcgattact
     2461 gggaagcggt gcgcgagttt tattatccgt ttgaagaagg catgaaggcc ggcacggccg
     2521 aggtgtacga gcacgaaatg cccggcgggc agtacacgaa cctgcgccag caggccaaga
     2581 gcctgcacct ggaaggccgc tggcacgaga tcgccgatgc gtatgcggaa gtgaacaaga
     2641 tgttcggcga catcgtgaag gtgacgccga gcagcaaagt ggtcggtgat ctggcgctgt
     2701 tcatggtgac caacaacctg tcgcccatgg acatcctggc gcccaaatca aaactgaatt
     2761 ttcccaagag cgttgtcgag atgatgcagg gctacctcgg ccaacccgaa ggcggctggc
     2821 ccaaagtgct gcagaagatc atcctcgatt ccggcg
//
LOCUS       DATIMH010000071         5617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_305683, whole genome shotgun sequence.
ACCESSION   DATIMH010000071 DATIMH010000000
VERSION     DATIMH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305683"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(546..917)
                     /locus_tag="VLI90_01215"
     CDS             complement(546..917)
                     /locus_tag="VLI90_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12848.1"
                     /translation="MTDQRASRGLGWTSIAIGLSEILAPSQLQRTMGIGNGQNTGIMR
                     VCGVREIAQGIDILAHDDPTPGVLARVAGDVLDGVLLGAAAMKTKRPGGFSAVCAMVL
                     GVVAMDVLYAQRLTRKRGLFS"
     gene            complement(1009..2202)
                     /locus_tag="VLI90_01220"
     CDS             complement(1009..2202)
                     /locus_tag="VLI90_01220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HSV12849.1"
                     /translation="MKALIWCAPYKVKIENVPEPKILNKRDAIVRITSTCICGSDLHL
                     VDGFVPFMKPGDILGHEPMGIVEELGPDVDKGKVKVGDRVVIPFPIGCGECFFCRRQL
                     WSLCDNTNPKPYIGETMLGQATAGIFGYSHLTGGFAGGQAEYLRVPLADVNCQVMPQE
                     FTDNQLVFLSDIFPTGYMAAQHCNIQPDDTVAVWGCGPVGQFTIRSAILLGAGRVIAI
                     DNGKMVPERLKMAADAGAITIDNRGDEGVYDRLMDLTGGIGPDVCIDAVGMEAHGSTP
                     LETFYDKVKTNLFMETERPAVIRQAINCCRKGGTVSIPGVYGGMSDKIPMGALMNKGL
                     TIKTGQTHVHRYVPELLDHIRAGRIDPSFVVTHRLPLSEAPHAYNIFREKKDGCIKVV
                     LDPAA"
     gene            complement(2297..3160)
                     /locus_tag="VLI90_01225"
     CDS             complement(2297..3160)
                     /locus_tag="VLI90_01225"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSV12850.1"
                     /translation="MTLMNSSDTRAGWPEAFGGAQQRRSKGGQQQQTQKETNVANTER
                     AVSVAAGSLLALLGLSRGTLPGLLGIAIGGSLIYRGATGHCSMYQTLGINTAEEERHR
                     QGTAAAGMNEVEEEISERGIHVEQAMLINKSPQELYQFWRNFENLPRIMTHLEAVRVS
                     DDRKSHWVAKTNRITGQVEWDAEITADEPNSRIAWRSLPGSDIDTTGEIRFSKAMGDR
                     GTEVHVTMEYVPPAGRMGHWFATLFGSSPRRMIRGDLQNFKRIMEVGEILTIIGQPHG
                     TCTGHGERYTE"
     gene            3535..4233
                     /locus_tag="VLI90_01230"
     CDS             3535..4233
                     /locus_tag="VLI90_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSV12851.1"
                     /translation="MNSHFARTIFAASLSAIAVVGVGCNKDDASAAHPAGSTPNVGVV
                     DLDRVGKAMGWQDEMQRNLQATDAELKRVIESRLHALQDTFEQKRKEIATADKLTAAQ
                     ITDLNNAKQKSDLEKLGLTPPQIDELVQASSAVQMEATSANNAYQQHMQQRAAAIQKA
                     YRDAVGPVVRRVAVANGRTVVFTPIDGLIYSDPANDLTDRVIDDLQKSPPIKVNLPEI
                     PHVVAATTEPTTQP"
     gene            complement(4376..5350)
                     /gene="argC"
                     /locus_tag="VLI90_01235"
     CDS             complement(4376..5350)
                     /gene="argC"
                     /locus_tag="VLI90_01235"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HSV12852.1"
                     /translation="MSIKVGIVGVSGYGGGEALRLCATHPAFEVTYVAGESSAGSRLI
                     EKFPGIGKLGELQIERWDAGKLPKLDVLFASLPSGESKEALARVPRDTKIVDIGGDHR
                     YADGWTYGLADVWPEKIKGQSRIANPGCYPSAALAAIAPLIANGIAKSSSQIIIDAKS
                     GVSGAGRGGGSTFGFAEVNEDVAAYGLLKHAHVPEMSRTIESLGVKGEAARIAFTPHL
                     IPMTRGILATCYLTGDATTEQCLTAARAFYANRPFVRVVEKPPHTKWALGTNLVFVSY
                     AAQAGGPIIALGAIDNLGKGAAGQAVQNANLITGQPETAGLEGTSLWP"
     gene            complement(5347..>5617)
                     /locus_tag="VLI90_01240"
     CDS             complement(5347..>5617)
                     /locus_tag="VLI90_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12853.1"
                     /translation="GWSYRRLNSRSIVAIGLMSYSLYVWQQPFLGAHITWPWEVNLLI
                     TFVVAAASYQLVEKPLRRLVRHRAALKPRATHVFAATVSPATLGA"
BASE COUNT         1030 a         1781 c         1743 g         1063 t
ORIGIN      
        1 ttggccggcg tgtgcggccg gacgagcgtg caatcgcggc tatcgacttg gaagttctcg
       61 cgctcgaagc cgttccacgt ttcggccagc gcggatgatg tcataacaaa caggacgagc
      121 gtggcgagaa gcaagagtga ttgcatgtgg tggcccgccg ccggcacgcg acgggtggcg
      181 catcctacga ggaaatgaat tcgtccgcca tcgccgcgag gagcgctaat ggcagggtga
      241 ccgcgtctgg ctttttcccc ctctcccggt agaccgggag agggtcgggg tgagagtgat
      301 ttcattcaat tgacaattga aaattgtcaa ttgccaattg aaaacgcaat caccctcacc
      361 ctaaccctct cccggcgtac cgggagaggg gaccggagag cgcactggct actgcgcttt
      421 gccttggcgt aaatggtgcg tgactcggtg cgtaccacgc atctcgcccg tgccgaaccg
      481 ccttcgacac gggcgagacg cccgtgctac aaatgaggga tctcgtgatc gcttgctctt
      541 gatgctcagg agaacaaacc gcgcttgcgc gtcaggcgtt gggcatacag cacgtccatc
      601 gcgacgacgc cgagcaccat cgcgcacacg gcgctgaatc caccggggcg tttggtcttc
      661 atggcggcgg cgccgagcag cacgccgtcg agcacgtcgc ccgcgacgcg cgcgagcacg
      721 ccgggggtgg gatcgtcgtg ggccaggatg tcgatcccct gggcgatctc gcgcacaccg
      781 cacacgcgca tgatgccggt gttctggcca ttgccgatgc ccatcgtgcg ctgcagttgg
      841 ctgggcgcga ggatttcgct gaggccaatg gcgatgcttg tccaacccaa tccgcgggag
      901 gctcgttggt cagtcatggg aactcctgtc ttgaatagcg atgagcgatg agcgatgaac
      961 gatgaagagg cgcctctcat cgctcatcgt tccacgttcc tcgttgcctt acgctgccgg
     1021 gtccagcacc accttgatgc agccgtcctt cttctcgcgg aagatgttgt aagcgtgcgg
     1081 cgcttcgctc agcggaagcc ggtgggtgac gacgaagctg ggatcgatcc ggccggcgcg
     1141 gatgtggtcc agcagctccg gcacatatcg gtgcacgtgc gtctggccgg tcttgatggt
     1201 caggcctttg ttcatcagcg cgcccatggg aatcttgtcc gacatgccgc cataaacgcc
     1261 cgggatcgaa acggtgccgc ccttgcggca gcagttgatc gcctggcgga tcaccgcggg
     1321 gcgctcggtc tccatgaaca ggttcgtctt caccttatcg tagaaggttt ccagcggagt
     1381 ggagccgtgc gcttccatgc cgaccgcgtc gatgcagacg tcgggaccga tgccgccggt
     1441 caagtccatc aggcggtcgt acacgccttc atcgccacga ttgtcgatgg tgatcgcgcc
     1501 ggcgtcggcc gccatcttca ggcgctcggg caccatcttg ccattatcga tcgcgatcac
     1561 ccggccggcg ccaagtaaga tcgcgctgcg gattgtgaac tgaccgacgg ggccgcagcc
     1621 ccagacggcc accgtgtcgt ccggctggat gttgcaatgc tgggcggcca tgtagccggt
     1681 cgggaagatg tcggacagga agacgagctg attgtccgtg aactcctgcg gcatcacctg
     1741 gcagttcacg tccgccagcg gcacgcgaag atattccgcc tgccccccgg cgaatccacc
     1801 ggtgaggtgc gagtaaccaa agatgcccgc cgtcgcctgg cccagcatcg tctcgccgat
     1861 gtatggcttt ggattggtgt tgtcgcacaa actccacagt tgccggcgac agaagaagca
     1921 ttcaccgcag ccgattggaa acgggatgac cacgcggtcg ccaaccttca ccttgccctt
     1981 gtccacgtcc gggccgagtt cttcgacgat gcccatcggc tcgtgcccga ggatatcgcc
     2041 cggcttcatg aacgggacga acccgtccac caggtgcagg tccgacccgc agatacacgt
     2101 tgacgtgatg cgcacgatcg cgtcgcgctt gttcaagatt ttcggttccg gcacattctc
     2161 gatcttcact ttgtatggcg cacaccagat gagggctttc atgttgggtt cctctgtttc
     2221 gttatgagtt caggtttccg ggcacctcga cacgccgcgt gaacgcggcc gctaatccaa
     2281 gcgggtgcgc cgcgcatcat tccgtgtacc gctcgccgtg tccggtgcag gtgccgtgcg
     2341 gctggccgat gatcgtgagg atctcgccga cttccatgat acgtttgaag ttctgcaggt
     2401 cgccgcgaat catccgccgc ggggatgaac cgaatagcgt ggcgaaccaa tggcccatcc
     2461 gcccggcggg ggggacgtac tccatcgtca cgtgcacctc ggtgccgcgg tcgcccatcg
     2521 ctttgctgaa gcgaatctca ccggtggtgt cgatgtcgga cccaggcagc gatcgccatg
     2581 cgatgcgcga gttgggctcg tcggcggtga tttccgcgtc ccattcgacc tgaccggtga
     2641 tgcgattggt cttggccacc cagtgcgact tgcggtcgtc gctcacgcgc accgcctcca
     2701 ggtgggtcat gatgcgcggg aggttctcga agttgcgcca gaactggtaa agctcttgcg
     2761 gcgatttgtt gatcagcatc gcctgctcga catggatgcc gcgctcagag atctcttcct
     2821 cgacttcatt cattccagca gcggcggtgc cttgtcggtg gcgttcctcc tcggcggtgt
     2881 tgatgccgag cgtttggtac atcgaacagt gaccggtcgc gccgcgatag atcaggctgc
     2941 caccgatcgc gatgccgagc aggcccggca gggtgccgcg cgacaggccg agcagcgcga
     3001 gcagcgagcc cgcggcgacc gagacagccc gctcggtgtt ggcgacattg gtctccttct
     3061 gcgtctgctg ctgttgcccg cctttagagc ggcgttgctg ggcgccgcca aacgcttcag
     3121 gccaaccggc tcgcgtgtcg ctgctattca tcaatgtcat cgtgctgctc ccttcgtgtt
     3181 gagggtcgag agttttcttg ggacatcaat tcatcagtcc gctttgccag cctggggagg
     3241 gaggaaatat cgtcgctgcc atccggcatt gcgtcgggat aatccgggca tccgatgggc
     3301 tggcgatggt gagtgacaga taacgcatcc atgcccggac ctcgcaagtt ggatcgccgg
     3361 cccacgcggc ggcgatccga ccgtgtcgtc gtttgcaaat gatgagccga ttcgcgctca
     3421 ttcgctaagg ttctgccgcg acgaatgatc gagcggacaa tttgaaaggc cgcaagggat
     3481 cggatatacc gccgtcgctt cacggcgctg tcatctatag agggaggcac atctttgaat
     3541 tcgcatttcg cgcgaactat attcgctgcc agtctgtcag cgattgccgt tgtgggcgtc
     3601 ggctgcaaca aggacgacgc cagcgccgcc cacccggcgg gcagcacgcc gaacgttggc
     3661 gtggtcgatc tcgaccgtgt cggcaaagcg atgggctggc aggatgagat gcagcgaaac
     3721 ctgcaggcga ccgatgcgga gctgaagcgc gtcatcgagt cgcgattgca cgcgctgcag
     3781 gacacattcg aacagaagcg gaaggagatc gcgacggccg acaagctgac ggcagcccag
     3841 atcacagacc tgaacaacgc caagcagaag tcggacttgg aaaagctcgg cctgaccccg
     3901 ccgcagatcg atgagctggt gcaggccagc agcgcggtgc agatggaagc gacgtcggcg
     3961 aacaacgcgt atcaacagca catgcaacag cgcgccgccg cgattcagaa ggcatatcga
     4021 gatgcggtgg gtcccgtggt gcgccgggtg gccgtcgcca atggacgcac ggtcgtcttc
     4081 accccgatcg atggcttgat ctactcagat cccgcgaatg acctcaccga tcgcgtgatc
     4141 gatgatctgc agaagtcgcc gccgatcaag gtgaacttgc cggagatccc gcacgtggtc
     4201 gcggctacga cagagccgac gacgcagcca taacaaaaag agtgcgccgc atacctccac
     4261 accgggttcg gagcgtactc gcgacgaagc gagattctgc ggcgcgagta gaacaaacat
     4321 ccgggtcgcc gcaagtatac ccggcgtgat tacttcaaca gctctgctcg taacctcacg
     4381 gccacagcga tgtcccttcc aaccccgccg tctccggttg ccctgtgatc aaattcgcgt
     4441 tctgcaccgc ctgcccggca gcgcctttgc caagattgtc gatggccccc agcgcaatga
     4501 tcggcccgcc cgcctgcgcc gcgtacgaca cgaacaccag gttcgtcccc agcgcccact
     4561 tcgtgtgcgg cggcttctcg acgacccgca cgaacggtcg attcgcataa aacgcgcgag
     4621 cggcggtgag acactgctcc gtcgtcgcat cgccagtcag gtaacacgtc gccagaatcc
     4681 cccgcgtcat cgggatcaaa tgcggcgtga acgcgatacg cgccgcttcg cctttcaccc
     4741 caagcgattc aatcgtccgc gacatctccg gtacatgcgc gtgcttcagc agcccatacg
     4801 cggcgacgtc ttcgttcact tcggcaaagc cgaacgtcga tccacccccg cggccggcgc
     4861 cgctgacgcc gctcttggcg tcgatgatga tctgcgacga cgacttggcg atgccattgg
     4921 cgatcagcgg cgcgattgcc gccagtgccg ccgacggata acacccgggg ttggcgatgc
     4981 gcgactgccc cttgatcttc tccggccaca catccgccag gccatacgtc caaccgtcgg
     5041 cgtaacgatg gtcgccgccg atgtcgacga tctttgtgtc cctcggcacg cgcgccagcg
     5101 cctctttcga ttcgcccgaa ggaagcgagg cgaagaggac gtcgagcttc gggagtttcc
     5161 ccgcgtccca gcgttcgatc tgcaactcgc caagcttgcc gatgcccggg aacttttcga
     5221 tgaggcgcga tcccgcgctg ctttcgcctg cgacgtacgt gacctcgaac gccgggtgcg
     5281 tcgcgcagag gcgcagcgcc tcgccgccgc cgtaacccga aacacccacg atgccgacct
     5341 tgatgctcat gcaccaagtg tagcagggga gacggtcgcg gcaaaaacgt gagtggcccg
     5401 cggtttcagt gccgcgcggt gccggaccag ccggcgcaac ggcttctcca ccagctgata
     5461 cgaagcggcc gcgacgacga aggtgatgag caggttcacc tcccacggcc acgtaatgtg
     5521 cgcgccgagg aacggctgct gccagacgta taggctgtac gacatcaacc cgatggcaac
     5581 gatcgaccgg ctgttcaacc tgcgatacga ccagccg
//
LOCUS       DATIMH010000072         4457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2615588, whole genome shotgun sequence.
ACCESSION   DATIMH010000072 DATIMH010000000
VERSION     DATIMH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615588"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="VLI90_01245"
     CDS             <1..1467
                     /locus_tag="VLI90_01245"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HSV12854.1"
                     /translation="PYVLRRHLEQNMQSKTLLAILSLLYFTVTTRAQTPPFYDWAPTP
                     PMGWNSWDCFGAGVTEAETMANADYIDQHLKSHGWNIVTIDIQWYEPLAHTDAYRSSA
                     ALEMDANGRLLPAANRFPSTKDTHSFKPIADALHAKDLKFGLHLLRGIPRQAVQQNVP
                     ILGADGVHAADIADTKDTCRWNGDMYGVDMTKPGAQAYYDSVFALLASWDLEFVKVDD
                     LSRPYHTAEIEAIRKAIDKTGRPMVFSTSPGETPLADGEHVANHANMWRVSDDFWDSW
                     PSLYEQFKRLNDWTPYRGPGHWPDADMLPVGNIRTWNPKQNKTHFTRDEQVTLMTLWS
                     IARSPLIVGANLPNNDEFTLALLSNDEVIAVDQHSTNNRQVSREKEHIVWVADVPGSK
                     DKYVALFNASPAPANDGRRRGPATTRAAVATAPTTPPDPAATQPTAVSVSLADLGLSG
                     PCEVRDLWTHRDLPRTTEAISVTVNSHGAALLRVHPAQ"
     gene            1737..2654
                     /locus_tag="VLI90_01250"
     CDS             1737..2654
                     /locus_tag="VLI90_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HSV12855.1"
                     /translation="MQPPGTYQLADVRMRDVCIRPDPATKTYYMVGPARRGVRLYTSK
                     DLRTWDGPQVIYRPPDDVWGDVQVFSIWAPEMHIYRGKYYLFMTFDTRHAFVEQWRNW
                     LPRVTRGSTIAVANSPTGPFTTFADHSTPPADMMTLDGTFWIEDGIPYMVFCHEWVQI
                     TNGSIDYIRLKDDLSGAVGEPTTLFHGSEAKWSHTGHDRGNNVTDGPYLYQSKSGKLF
                     MIWSSGGAGGYVCGIAISPSGKLAGPWVQQDEPLYSHDGGHGMLFTTFGGQLMMVLHS
                     PNGREARPRIFEMEDTGETLKISREFTGE"
     gene            complement(2776..>4457)
                     /locus_tag="VLI90_01255"
     CDS             complement(2776..>4457)
                     /locus_tag="VLI90_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12856.1"
                     /translation="EAELIAKAMHTYLWLPEAGTLAEFKDLLGHQLVHPSAGLWSFYH
                     TIDSEVPTPAEARQMAMALQRAMPHMPVHGPGVPDDRPYFVLPETQWMPYSWSVNNVV
                     MGENLHTALALWQAGDAEQAFTMAKGALLASMYMGICPGNVGTMNYLDVYRRESQRDF
                     ADGCGVMSRAIVQGLFGVKPDALAGELRISPGFPRDWNHATLTHPDVHVEFTREGLSD
                     HWSVGSTSAAFRAIKLRVPAIRERVARVEINDVESKWRRDDDAVGGPRIELDLPGGRV
                     STAVIQWSGDAITPMTIARSMAPADEPVPAFDWRLPRPSAKCEPVDLSKTFNDRVSSI
                     FAAGKYRSPRSPGDSLAIPSQGAGAWAGHVNELPAIDDTGLRNVAAKSGGRLVMPNGV
                     PFATPGPGTSPNICFTSQWDNYPREVTMPLTGRASRAYLLMAGSTNFMQSRLDNGEVL
                     VTYTDGLTSRLALRNPTTWWPIEQDYFIDDYQFRYDGSLPPRVNLKTGEIRLLDEQTF
                     KGKGGPVPGGAATVLQLPLDAGKELQSLTVRTLANEVVIGLMAVTLERAEN"
BASE COUNT          849 a         1496 c         1378 g          734 t
ORIGIN      
        1 ccgtacgtac tgcggcggca tctggaacaa aatatgcaat cgaagacact actcgcgatt
       61 ctctcgctcc tctatttcac agtgaccacg cgcgcccaaa ccccaccctt ctacgactgg
      121 gcccccaccc cgccgatggg ctggaactcc tgggactgct tcggcgcggg cgtcaccgaa
      181 gccgaaacga tggccaacgc cgactacatc gaccaacacc tcaagtcaca cggctggaac
      241 atcgtcacga tcgatatcca gtggtacgag ccgctggcgc acactgacgc ctatcgctcc
      301 agcgccgcgc tggagatgga cgcgaacggc cggctgctgc ccgccgccaa tcgcttccca
      361 tcgacgaaag acacgcactc gttcaagccg atcgccgatg cgctgcacgc caaggacctg
      421 aagttcggcc tgcacctgct ccgcgggatc ccgcgccagg cagtgcagca gaacgtgccg
      481 atcctcggcg ccgatggcgt gcatgccgcc gacattgccg acaccaaaga cacttgccgc
      541 tggaacggcg acatgtacgg cgtcgacatg accaagcccg gcgcgcaggc ttattacgat
      601 tcggtcttcg cgctgctcgc gtcgtgggac ctggagttcg tgaaggtcga tgacctctcg
      661 cgcccgtatc acaccgcgga gatcgaggcg attcgcaagg cgattgacaa gaccggccgg
      721 ccgatggtct tctcaacttc gcccggcgaa acgccgctcg ccgatggcga gcatgtggcg
      781 aaccacgcga acatgtggcg cgtgagcgac gacttctggg acagctggcc gtcgttatac
      841 gagcagttca aacgcctgaa cgactggacg ccctaccgcg gccccggcca ctggccggac
      901 gccgatatgc tgccggtcgg caacatccga acgtggaatc caaagcaaaa taagacgcat
      961 ttcacgcgcg acgagcaggt gacgctgatg acgctgtggt cgatcgcgcg ctcgccgctg
     1021 atcgtggggg cgaacctgcc gaacaacgat gagttcacgc tggcgctgct gagcaacgat
     1081 gaagtgatcg cggtggatca gcactcgacg aacaaccggc aggtttcgcg cgagaaggag
     1141 cacatcgtgt gggtcgcgga tgtgccgggt tcgaaggaca agtacgtggc gctgttcaac
     1201 gcgtcacccg cgccggcgaa tgacgggcga cgccgaggcc ccgcgaccac gcgcgccgcg
     1261 gtggccacgg cgccaaccac gccgccggac cccgcggcga cgcaaccgac ggcggtgtca
     1321 gtgtcgctgg ccgaccttgg cttgagtgga ccgtgcgagg tgcgcgacct gtggacgcac
     1381 cgggacctgc cgcggaccac cgaagcgatt tccgtgaccg tcaactcgca cggcgcggcg
     1441 ctgcttcgcg tgcatccggc gcaataggtg tcggatcaga ccacgccggg ttccgagcgt
     1501 actcgcgacg aagcgagatg ctgcggcgcg ataccggaga tccgggtcgt cggagtacct
     1561 ccgaacctgg cgtgggtgaa tgaccatcga caggagcacg aactatgacg gcgaacacca
     1621 taatcgcacg cgcggcgctc cttctgcttt gcgtcggcac aggcgcacaa gcacaaaccg
     1681 gcatcccccc acgcccgagt cccgggccca ccaccgggcc aacatcccgc ttcatcatgc
     1741 agccgccggg gacgtaccag ctggcggacg tgcgaatgcg cgacgtctgc attcgccccg
     1801 atccggcgac caagacgtac tacatggtcg gccccgcgcg ccgcggggtg cgcctgtaca
     1861 ccagcaaaga cctgcgcacg tgggatggcc cgcaggtgat ttatcgcccg ccggacgacg
     1921 tgtggggaga cgtgcaggtc ttcagcatct gggcgccgga gatgcacatc taccgcggga
     1981 agtattacct gttcatgacc ttcgacacgc ggcacgcatt cgtcgagcag tggcggaact
     2041 ggttgccgcg cgtgacgcgc ggctcgacga ttgccgtggc gaattcaccc accggaccgt
     2101 tcaccacatt tgcggatcac tcgaccccgc cggcggacat gatgacgctg gacggcacct
     2161 tctggatcga ggacggcatc ccctacatgg tcttctgcca cgagtgggtg cagatcacca
     2221 acggctcgat cgactatatc cgactgaagg acgatctgtc cggagcggtc ggtgagccaa
     2281 cgacgctgtt ccacggcagc gaggcgaagt ggagccacac cggccacgat cgcggcaata
     2341 acgtcaccga cgggccgtac ctgtaccaaa gcaaatccgg gaagctgttc atgatctggt
     2401 ccagcggcgg cgccggcggg tacgtctgcg gtatcgcgat ctcccccagc ggcaaactcg
     2461 ccggcccgtg ggtgcagcag gacgagccgc tgtactcgca tgatggcggg catggcatgt
     2521 tgttcacgac gttcggagga cagctgatga tggtgctgca ttcacccaac gggcgagaag
     2581 cacgaccgcg gatttttgag atggaagaca cgggcgagac gctgaagatc tcgcgcgaat
     2641 tcacgggcga gtgagagaag cgcacgcacc gaccacgccg ggttcggagc gtactcgcga
     2701 cgacccggat tctttgcctc ccgcgcgaaa catccgggtc gtcggagtac ctccgaaccc
     2761 ggcgtgggga atggatcagt tttctgctcg ttccaacgtt acggccatca atccaatcac
     2821 cacctcattc gccagcgtgc gcaccgtcag cgattgcaat tccttccccg cgtccagcgg
     2881 cagttgcaga acggtggccg ccccgccggg gacggggccg cctttgcctt tgaacgtttg
     2941 ctcgtcgagc aggcggattt cgccggtctt caaattcacg cgcggcggca gcgaaccgtc
     3001 gtagcggaat tggtaatcat caatgaaata atcctgttcg atcggccacc acgtggtggg
     3061 attgcgcagc gccagccgag aggttaaacc atcggtgtac gtaacgagca cctcgccatt
     3121 atcgaggcgg ctctgcatga agttcgtgga cccggccatc agcaggtagg cgcgcgaggc
     3181 ccggccagtg agcggcatcg tcacctcgcg cgggtaattg tcccactgcg acgtgaaaca
     3241 gatgttagga gatgtgcccg gcccgggcgt cgcgaacggc actccgttgg gcatgacgag
     3301 gcgaccgccg gattttgcgg cgacgttgcg caagccggta tcatcgattg ccggcaactc
     3361 attcacgtga cctgcccatg cgccggcgcc ttgcgatggg atcgccagcg aatcccccgg
     3421 cgagcgtggc gaacgatatt taccggcagc gaaaatgctc gacacgcgat cgttgaacgt
     3481 cttcgacaga tcgaccggct cgcacttcgc gctcggccgc ggcagccgcc agtcaaacgc
     3541 ggggaccggt tcatccgcgg gcgccattga tcgcgcaatc gtcataggtg tgattgcatc
     3601 gcccgaccac tgaatcactg ccgttgaaac ccgcccacca ggcagatcaa gctcgatcct
     3661 cggaccgccg acggcgtcat catcccgccg ccacttcgac tcgacgtcgt tgatctcgac
     3721 gcgcgccacg cgctcccgta tcgccggcac gcgcagcttg atggcgcgaa atgcagcgct
     3781 cgtcgagccg acgctccagt ggtcgctcaa tccctctcgt gtgaactcga catgcacatc
     3841 cggatgcgtc agcgtcgcgt ggttccagtc gcgcggaaag cccggcgaga tccgcagctc
     3901 gccggccagc gcatccggct tcacgccgaa cagtccctgc acgatcgcgc gcgacatcac
     3961 gccgcagccg tcggcgaagt cccgctgcga ttcgcggcga tacacgtcca ggtaattcat
     4021 cgtgccgacg ttgcccgggc agatgcccat gtacatgctg gccagcagcg cgcctttggc
     4081 catcgtgaag gcctgctcgg cgtcgcccgc ctgccacagc gccagcgccg tgtgcaggtt
     4141 ctcgcccatc accacgttgt tgaccgacca cgaatagggc atccactgcg tctccggcag
     4201 cacgaaatag ggccgatcgt ccggcacgcc cggcccgtgc accggcatat gcggcatcgc
     4261 ccgctgcagc gccatcgcca tctgccgcgc ctccgccggc gtgggcactt cagaatcgat
     4321 cgtgtgatag aagctccaca gcccggcgct gggatgcacg agctgatggc cgagcaggtc
     4381 cttgaactcg gccaacgttc ccgcctccgg cagccagagg tacgtgtgca tcgccttggc
     4441 gatcagctcc gcctcgc
//
LOCUS       DATIMH010000073         5086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_882986, whole genome shotgun sequence.
ACCESSION   DATIMH010000073 DATIMH010000000
VERSION     DATIMH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882986"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..1307)
                     /locus_tag="VLI90_01260"
     CDS             complement(321..1307)
                     /locus_tag="VLI90_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12857.1"
                     /translation="MPETIDIDADPFLKLLTDALRAGPGSPDWHQAVSRLRSGGERNL
                     DEYNMLIAARERLESGRGYREIRPGPAFTRKVMDGIDAEPPPGRRALPTANLIAILGA
                     AVVLVVLASVGFWLLHGPPAANPGEDLSNAYFGTTVADATFNAPLPPAWRTLGPAPLD
                     PARGLRPTTLPATTASSADYDGGGVVLTNGIPADQAFAVEASFRIQHVTDDVIPELFV
                     TDQPDFDADRATSAHELVWLVREGHAKVVLPDQQFAGSSEKVTDGQSLTVRAIIGQNT
                     AQLVSGRHTIWSGPSQLAGDRPRYVGIRVLRRGNEKKDRDAVIVQSVKVMQR"
     gene            complement(1332..1961)
                     /locus_tag="VLI90_01265"
     CDS             complement(1332..1961)
                     /locus_tag="VLI90_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV12858.1"
                     /translation="MVDEVQDLAAVPLGRASDEELMRRTQQGDKQAFSLIYERYSASV
                     LSYLYRMLGSVEDVESIGQEVFLRAFRFAPTYRYPQKLSTWLFTITRNLAINQSRRRK
                     RSPVRNITELNLEGIDMSGDPYQVATRATDDVERREEIARVLKALDELPTDQKEVIVL
                     GVFQDLSYAQMEEITGTKAVTLRSRMFHGLKRLGRMMGGTVDESKGDAV"
     gene            2357..3022
                     /locus_tag="VLI90_01270"
     CDS             2357..3022
                     /locus_tag="VLI90_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV12859.1"
                     /translation="MSLLVLGMSVAPATGAATTGITTPYFSSANSPLVTASPGYQLET
                     FESGALTLPAVTLTTLHGSSVDLHSSVDGDDGVLDGDGFNGKSLTVFTQANTTGATFS
                     FNNIMLGTYPKSAGVAVTVDIANPLIFTVFDSSNNVSGTITVPNSNTLDPSNDDFLFW
                     ASDPAGISAISVSSNNAAIHLHLDHLQFDTINTISIPEPGTLAGATIAGGVVCIGRRR
                     RRV"
     gene            3083..4102
                     /locus_tag="VLI90_01275"
     CDS             3083..4102
                     /locus_tag="VLI90_01275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV12860.1"
                     /translation="MPAERKTISIVIPCYNEAEAFARMREALVSLADSLEPDFNVELV
                     FIDDGSRDQTWRAISEFARDDARVRGVALSRNFGHQFALTCGYDLARGDAVVSMDADL
                     QDPPETVRQLIEQWLAGYDVVFAVRRQRAGESWFKLCTASCFYRLIRAMGATDVRANV
                     GDFRLMSRAAVDALNRMREQHRFIRGMVGWVGFRTTEVLYDRLPRVAGETKYPLRKMV
                     KLAIDAIISFSSVPLRVAYWTGVALMIASFAYLAYTVVQHWLFGVALVPGWSSLLVAV
                     VAFGTMNLICLGLIGEYIGRIYEQSKQRPLYLIRDAIENPNVIAGSGSPGLSRSIKTG
                     ASAPG"
     gene            4130..4942
                     /locus_tag="VLI90_01280"
     CDS             4130..4942
                     /locus_tag="VLI90_01280"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV12861.1"
                     /translation="MNPSATDDLHDKESHFHDAWADSVRPEDVRVRESFEAPTAFENR
                     EILRRMGDLRGKRVLDIGGGLGESSVYFAIQGADVTYTDLSPGMADFASRLAAHHGVK
                     IHTAVCAAEAVDTLGGDFDFVYIANTIHHVHDRAALLRAMHAALKPGGRFFSWDPIAY
                     NPVINVYRRMATDVRTEDESPLTFADLKLARTLFRDVGHREFWVSTLSLFLKYYLIDR
                     VHPNQDRYWKRILRETPRSLWWWRPLSALDAALTRLPLVRRLAWNMVMWGTK"
BASE COUNT          888 a         1580 c         1628 g          990 t
ORIGIN      
        1 tgaggatttc ctcgatcacg cgccgctgac cgacaatcac tttcccgagt tcggattcga
       61 ggtgttggta cgcttcgccg aggtagcgcg ccgcgtcgag gtcagatgcg gagccggcgg
      121 cggcgggatc gatggaaccg gtgacggaca tcgaggaact ccgatgagac gcacgcacgc
      181 gtcgtgtctt accccctcgc ccacgtactc gtgggagagg gacggggtga gggtgattta
      241 gggtgcgatc ggctctcgaa attcaaaatc accctcaccc tcaccctctc ccacgagtac
      301 gtgggcgagg ggaccagaag ctaccgctgc atcaccttca cggactgcac gatcaccgcg
      361 tcacgatcct tcttttcgtt gccgcggcgc aggacgcgga tgccgacgta gcgcgggcga
      421 tcgcccgcca attgactggg accggaccag atcgtgtgtc ggccactgac cagctgcgcc
      481 gtgttttggc cgatgatggc gcgcacggtc agcgattggc cgtcggtcac cttctcgctc
      541 gatccggcga actgctgatc gggcagcacg accttcgcgt ggccttcgcg caccagccat
      601 accagttcgt gggcgctggt cgcgcggtcg gcatcgaagt cgggttgatc ggtgacgaac
      661 aattcgggaa tcacgtcatc ggtcacgtgt tgaattcgaa aggaggcttc gaccgcgaag
      721 gcttggtcgg cgggaatgcc gttggtgagc acaacgccgc caccgtcata atcggcggat
      781 gatgcggtgg tcgcgggcaa ggtggtgggt cgcaagccgc gcgcaggatc aagcggggcc
      841 gggccgagcg ttcgccacgc cgggggcagc ggcgcgttga acgtcgcatc ggcgaccgtg
      901 gtgccgaagt atgcgttgga gagatcttcg cccggattgg cggccggcgg accgtgcagc
      961 agccagaatc caacggacgc aagcacgacc agcacgaccg ccgcgccgag gatcgcgatc
     1021 aagttcgccg ttggtaatgc acggcggccg ggcggtggtt cggcgtcgat gccgtccatc
     1081 accttgcggg taaacgcggg acccgggcgg atctcgcgat acccccggcc gctttccaag
     1141 cgctcgcggg cggcgatcag catgttgtat tcgtcgagat tgcgctcgcc gccgctgcgc
     1201 aggcgcgaca ccgcctggtg ccaatcgggg ctgccgggac cggcgcgcag cgcatcagtc
     1261 agcagcttga ggaacgggtc cgcgtcgatg tcaatcgtct ctggcattgt tgtggttgga
     1321 tgggcgcagt cctacaccgc gtcgcccttc gattcgtcaa ccgtgccacc catcatccgg
     1381 cccaggcgct tcaggccgtg gaacatccgc gaccgcagcg tgacggcctt ggtgccggtg
     1441 atctcttcca tctgcgcgta cgacagatct tggaacacgc ccagcacgat gacttccttc
     1501 tggtccgtcg gcagctcgtc cagcgccttg agcacgcgcg cgatctcctc gcgacgttcc
     1561 acgtcatcag tggcgcgtgt ggcgacctga taaggatcgc cgctcatgtc gatgccttcc
     1621 aggttcagct ccgtgatgtt ccgcaccggg ctgcgcttgc gccgacggga ttgattgatc
     1681 gccaggtttc gcgtgatcgt gaagagccag gtcgagagct tctgtggata gcgataggta
     1741 ggcgcgaagc ggaaggcgcg gaggaagact tcctgcccga tcgactcgac gtcttcgacg
     1801 ctgccaagca tgcgatagag gtacgacagg actgacgcgc tgtagcgttc gtagatcagc
     1861 gaaaaggctt gcttgtcccc ctgctgcgtg cggcgcatca gttcttcatc cgaagcacga
     1921 cccagcggca cggcggctag gtcctgtacc tcgtcaacca ccggggggac ctttggtttg
     1981 gctgccggcg ccgcgatgga cgcggtcgcg gtggctgttc tggcgtttgg cttatccgcc
     2041 cgtcgctgca ttcacgctcc gctggaaccg ccgggatgaa agaccgttac gagcgatgct
     2101 cgtgacgccc aagctgccga atggcgggtt ggccatgtta tccgaaagcg gagcagtcgt
     2161 cacgaatacg ggtgtagatc acattagccg gcgccagtag ttcatcgcgg acacgcgcgc
     2221 catcgctttt tcgttgacat ggccacgcaa caacttacaa ttacggtcgt tataagcatc
     2281 ttttccgcat ttctggagga gcgagagcat gggcacgtac gcaacattgt ctagagcgtc
     2341 attgttagct tcgtctatgt cacttttggt gctggggatg tcggtagcgc cggcaacagg
     2401 cgcggccacg accggcatca ctacgcctta tttcagcagt gccaacagcc cgctggtcac
     2461 ggcttcgccg ggttaccagc tggagacgtt tgagagtggc gctctgactc tgcctgccgt
     2521 cacccttacg acattgcatg gctcgtccgt cgacctgcac agttccgtcg atggtgatga
     2581 tggggtgttg gacggcgacg gtttcaacgg caaatcgctc accgtcttta cgcaagccaa
     2641 caccacgggc gccactttca gcttcaacaa catcatgctc ggcacttatc ccaaaagcgc
     2701 cggggtggcc gtcacggtgg acattgccaa cccgctgatc ttcaccgttt ttgacagcag
     2761 caacaacgtc tctggcacaa tcaccgttcc caacagcaac acgcttgacc cgtcgaacga
     2821 cgacttcctg ttctgggcat ccgaccccgc cgggatcagc gccatcagcg tgtcgtcgaa
     2881 caacgcggcc atccacctgc acctggatca cctgcagttc gacacgatca acacgatttc
     2941 gattcccgaa cccggcaccc tcgccggggc aacgatcgcc ggcggtgtcg tgtgcattgg
     3001 ccggcgtcgc cggcgcgttt gaggctggca actcagcggg taacatagcc gcgcgcgatt
     3061 gaccgcgcgg agtaataccc ggttgcccgc tgagcgaaaa acgatttcga ttgttatccc
     3121 ctgctacaac gaagccgagg cgttcgcgcg catgcgcgag gcgctggtct ccctagcgga
     3181 ctcgttggag ccggacttca acgtcgagct ggtcttcatc gacgacggca gccgggacca
     3241 gacttggcgg gcgatcagcg aattcgcccg cgacgacgcc cgcgtgcgcg gcgtcgcgct
     3301 gagccggaac ttcggccatc agttcgcgct gacctgcggc tacgacctgg cgcgcggcga
     3361 cgcggtcgtc tcgatggacg ccgacctgca ggacccgccc gagaccgtgc ggcaactgat
     3421 cgagcagtgg ctcgccggat acgatgttgt cttcgcggtc cgccggcaac gcgcgggcga
     3481 gagctggttc aagctctgca ccgcgtcctg cttctaccga ttgatccgcg cgatgggggc
     3541 gacggacgtt cgcgccaacg tcggcgattt ccggttgatg agccgggccg ccgtcgacgc
     3601 gctcaaccgc atgcgcgagc agcaccgttt catccgcggc atggttggct gggttggttt
     3661 tcgcacgacg gaggtactgt acgaccggct gccgcgcgtt gcgggcgaga cgaaatatcc
     3721 gctgcggaag atggtgaagc tggcgatcga cgcaatcatc tcgttcagct ccgtgccgct
     3781 gcgcgtcgcg tattggaccg gcgtggcgct gatgattgcc agcttcgctt acctggcgta
     3841 cacggtcgtc cagcattggc tgttcggcgt ggcgctggtg ccggggtggt ctagccttct
     3901 cgtcgccgtc gtcgcgtttg ggacgatgaa cctcatctgc cttggcctga tcggcgagta
     3961 catcgggcgc atttacgaac agagcaaaca gcggccgctg tatttaattc gagatgcgat
     4021 cgagaatcca aacgtcatag ccgggagcgg aagccccggt ctgtctcgat cgataaagac
     4081 cggagcttcc gctcccggct aataagtgga cgcgcatacg ttgtattcga tgaatccttc
     4141 cgccaccgat gatcttcacg acaaggaatc gcacttccac gatgcctggg ccgactcggt
     4201 ccggccggag gatgtgcgcg tgcgcgagtc cttcgaggcc cctaccgcgt ttgagaaccg
     4261 cgagatcctc cgccggatgg gcgacctgcg cggcaagcgc gtgctcgaca tcggcggcgg
     4321 cttgggtgaa tcgtccgtct atttcgcgat tcagggcgct gacgtgacgt acaccgatct
     4381 gtcgcccggc atggccgact tcgcatcgcg cctggcggct catcatggcg tcaagatcca
     4441 caccgccgtc tgcgccgccg aagccgtcga tacgctcggc ggcgatttcg acttcgtcta
     4501 catcgccaac acgatccacc acgtgcacga tcgcgccgct ttgttgcggg cgatgcacgc
     4561 ggcgctgaag ccgggtggac gattcttttc gtgggatccg atcgcgtaca acccggtgat
     4621 caacgtctat cgccggatgg cgacggacgt gcgcactgaa gacgaaagcc cgctgacgtt
     4681 cgcggacctg aagctcgcgc ggacgctttt tcgagacgtc ggccatcgcg agttctgggt
     4741 ctcgacgctg tcgctgttcc tcaaatatta cctgatcgac cgcgtgcacc cgaaccagga
     4801 ccggtactgg aaacgcatcc tgcgcgagac gccaagatcg ctgtggtggt ggcgaccgct
     4861 gagcgcgctc gacgcggcgc tgacgcgatt gccgctggtg cgccggctgg cgtggaacat
     4921 ggtgatgtgg gggacgaagt agtcgcagtc atcctgagcg ccgcacgtac tcgtgcgcag
     4981 tcgaaggatc ttgccgagaa gtaagtgaac gttcggatgc gcgaacggcg cagcagtcgc
     5041 ttgcggtttc gcagaccagt gacggcggct tgtccgtctg cgaaac
//
LOCUS       DATIMH010000074         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2615831, whole genome shotgun sequence.
ACCESSION   DATIMH010000074 DATIMH010000000
VERSION     DATIMH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615831"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..916
                     /gene="hisN"
                     /locus_tag="VLI90_01285"
     CDS             134..916
                     /gene="hisN"
                     /locus_tag="VLI90_01285"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HSV12862.1"
                     /translation="MSHSPSLSDLLAVAMDAAYAGGRRTLGYFNTNVEVETKSDNTPV
                     TRADREAEAEIRRIIARHYPSHAVLGEETGGTPGDAGYRWIIDPIDGTKTFIHGVPFY
                     GVLIAVEVAGQPAVGVVYLPALDEMVAAASGLGCGWNGRPARVSTVDRLEDATLLTTN
                     VTRSQARSDAYDRLVAKTKLHRTWGDCYGYVLVATGRAEIMLDAAMNPWDCAPLLPIL
                     TEAGGHFSAWSGEVTIYGPDAVGTNAALHAEVLSILKTEKRR"
     gene            complement(929..1834)
                     /locus_tag="VLI90_01290"
     CDS             complement(929..1834)
                     /locus_tag="VLI90_01290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV12863.1"
                     /translation="MSPAVSVLMPVYNAQRYIASAVQSILGQTFTDFELIIVDDGSTD
                     RSREILRGFAARDPRVKLISRPNTGYVVALNEALATATGEFIARMDADDMSLPYRFER
                     QVEYLRAHPDCVLVGTHVVTMDSDGSLIGVMPDIAFGHQRIDDALLRRGWPIVHPAVM
                     MRARALREIGGYKVEYCPNEDHDLFLRLAEFGQIENLPEVLIHYRKHAASVSAMNNDR
                     MTANVGKIIVEACRRRGVPVPPEVAQTQPRPQLRTADVQRVWAWMAMKNHNIRTARKY
                     ALATLCRRPLSMDSWRLTFCAVRGH"
BASE COUNT          406 a          668 c          797 g          420 t
ORIGIN      
        1 actcccacgt aaacccggcg tgtgttcacg tcgggctaaa cggggtactt ttcatttgat
       61 cgcgggcgcg cacgcgcgca tgcgtcggga tctgatcttg ccggacgcgt gttcgtcggt
      121 tctcatccgc tgcatgtccc attcgccttc gctgtcagat ctgctcgccg ttgccatgga
      181 tgccgcttac gccgggggtc ggcggacgct ggggtatttt aatacgaacg tagaagttga
      241 aactaaatcg gacaatacgc cggtgacgcg ggcggatcgc gaggcggagg cggagattcg
      301 gcggatcatc gcacggcact acccgtcgca cgcggtgctg ggtgaagaga ccgggggaac
      361 gccgggggac gcgggatatc gctggatcat cgatccgatc gacggcacga agaccttcat
      421 ccacggcgtg ccattttatg gcgtgctgat tgccgtcgaa gtcgcggggc agccggcggt
      481 gggcgtcgtt tatctgccag cgctggatga gatggtcgcc gcggcgagtg gattgggctg
      541 cggttggaac ggtcggccgg cgcgggtttc gactgtcgat cgcctggaag acgcgacgct
      601 gctgacgacg aacgtgacgc gcagccaagc gcgatcggac gcgtacgacc ggctggtggc
      661 gaagacgaaa ctgcaccgta cctggggcga ttgttacggc tacgtgctcg tggccaccgg
      721 acgggcggag atcatgctgg acgcggcgat gaacccgtgg gactgcgcgc cgctgctgcc
      781 gatcctgacc gaagccggcg ggcacttcag cgcgtggagc ggggaagtga cgatctacgg
      841 gcccgacgcg gtggggacga acgcggcgct gcacgcggaa gtgctgtcga ttctgaagac
      901 ggaaaagcgg cggtgactct tagcccgttc aatggccgcg cacggcgcag aacgtgaggc
      961 gccacgaatc catcgacaac gggcggcggc atagggtggc cagcgcgtat tttctagccg
     1021 tgcggatgtt gtggttcttc atcgccatcc acgcccagac gcgctgcacg tcggccgtgc
     1081 gcagctgcgg acgcggctgc gtctgcgcga cctccggggg caccggaaca ccgcggcggc
     1141 ggcaggcttc tacgatgatc ttcccgacgt tcgctgtcat gcgatcgttg ttcatcgccg
     1201 acacgctggc ggcgtgcttg cggtagtgga tcagcacctc gggcaggttc tcgatctgcc
     1261 cgaattccgc caaccgcagg aacaggtcgt gatcttcgtt ggggcaatac tcgaccttgt
     1321 acccgccgat ttcgcgcagc gctctggcgc gcatcatgac ggccgggtga acgatgggcc
     1381 aaccgcggcg gagcagcgca tcgtcgatgc gctgatggcc gaacgcgatg tcgggcatca
     1441 cgccgatgag cgagccgtcg gaatccatcg tgacgacgtg cgtgccgacc aatacgcaat
     1501 ctggatgggc gcgaaggtat tcgacctgcc gctcgaagcg gtacggcagc gacatgtcgt
     1561 cggcgtccat gcgggcgatg aattcgcccg tggcggtggc cagcgcttcg ttgagcgcga
     1621 cgacgtatcc ggtgttgggc cggctgatca acttgacgcg cgggtcgcgc gcggcgaagc
     1681 cgcgcaggat ctcgcgcgat cggtcggtcg atccatcatc gacgatgatc agctcgaagt
     1741 cggtgaaggt ctgcccgaga atgctctgca ccgccgacgc gatgtagcgc tgggcgttgt
     1801 agaccggcat caaaacgctg acggcggggc tcattggtcc atcactccga acaggcaatg
     1861 cgggaacagc aggtcggcgg ccatcatcca tgtacgtccg cgaaggcgca atcctgcgtc
     1921 gtagccgtcg gttggtcaag cggcgaattt cacgggcctt gcggcggcac ttcaagcggc
     1981 gagggtctcg ggcagcgcgc gttgaggtcg aacgacagta tgccatcgcg cccgacacca
     2041 tgccaatttg aaccgacgaa gtgacggcgc ctcgggcggt tcgtatcgcc ggtggcggat
     2101 gtatggaagc ggacccagcg attttaaaag ataaatgcgg ttgccgggca aatcaggttg
     2161 aaagtccccg caccgacccc gtagtctgcg cgtacagaag tttattgcgc ggcgcagacg
     2221 ggtgagctgc tcgtcctgaa tcctgttttc ataacggata gattgtccga ccgtggccgt
     2281 ggccgggcaa t
//
LOCUS       DATIMH010000075         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_306413, whole genome shotgun sequence.
ACCESSION   DATIMH010000075 DATIMH010000000
VERSION     DATIMH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306413"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VLI90_01295"
     CDS             complement(<1..1342)
                     /locus_tag="VLI90_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12864.1"
                     /translation="MVALLGSMPMLARAGDTVPIQEFVRHPQYTATKISPDGRFLALA
                     MQQGDVMSLAVLRMKDMQVIRITRLTGGESVGSFYWVGDNRIMYTSAKNFGSYAVPFG
                     TGEWYAMDSDGGRPRTLVSYNTSATEGQSKIMHFHESLSMLDPLPNDESKALMQLNDS
                     STGGRNAVVAIDTMTGRRQVLARAPRDDCEMVLDAANQPRYANCTETKDAKLGYNEHT
                     ELYRLDGDTWTRIDKSNKDEPAMRVVGTAADGRIYAMAGDGKTPSAFGILDPATNAFH
                     PLYRDPDTAIHGFLGASDEKTIVGVVTMAGAPHVELVDKKNPDAAVYVSLSKAFPGEL
                     VDITSATRDGKQMIVAVSSDTDPGQLYLFDRDSKNVRFLMKSRPALDPAVMAHVQPFS
                     FKARDGRTLYGYMTVPNGKSKDLPTIIHPHGGPIGIRDDWGFDPESQMLANRGYL"
     gene            complement(1408..3420)
                     /locus_tag="VLI90_01300"
     CDS             complement(1408..3420)
                     /locus_tag="VLI90_01300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HSV12865.1"
                     /translation="MRILVLAAALLFAGAAQAATAVDVASFVKRDSFDQIKLSPNGDY
                     YAASVPGEDSSVLVIVRRADNKVTGGFGIGRNTYISDFAWVNPQRVVFGTARRFGALD
                     NPQLTGNLYAMNADGTGKDILVGQDVDVMSTGSHIQTKKTEMVAAFLLDDLPADDHNV
                     LISVSGFSGDSYNRVEKMDVYSGRRVPVARSPVRNSDYVTDADGVVRFAFGYETDNDH
                     KLFYRAKTKVDWTNTADWREINDESSTGHIEVPLGFSADGNIAYLQVEQANGPDSIVA
                     LDTRDDTRKTVLRDDDSDPAAIIRSMGTPYPAPVGAFIDDGKPRTQFFDDASPEARLY
                     RSLEAAFPGDLVEITSKTADGALALVRVSSDRDAGEFYLFDTVNKKAGYLLAPRQWQD
                     PATMSEQRPFAFKARDGRMLHGFLTIPRGSDGKHLPTVVIPHGGPFFVADHWGFDTET
                     QLIAAAGYAVVQVNFRGSSGYGRDFTQAGARQWGRALQDDITDATHWVVDQGYADPTR
                     ICIYGASYGGYAALMGLAREPSLYKCAAGYAGVYDLPLMFQKGDTQALDSGKAYLRGW
                     IGDPTQLGAVSPVNLAGQIKAPVFLAAGGADERAPIAHSERMEKALRAAGVPVETLYF
                     RTEGHGFYTDEHRIAFYTQLLAFLHRNIGGGAGVATTTGAAAAAAH"
BASE COUNT          597 a         1187 c         1142 g          704 t
ORIGIN      
        1 cgaggtagcc gcggttggcc agcatctggc tttccggatc gaatccccag tcgtcacgga
       61 ttccgatcgg gccaccgtgc ggatggatga tcgtcggcag gtctttcgat ttgccgttgg
      121 gcaccgtcat gtagccgtac agcgtacggc cgtcgcgcgc cttgaacgag aacggctgca
      181 cgtgcgccat cacggccgga tccagcgccg ggcggctctt catcaggaag cggacgttct
      241 tgctgtcgcg gtcgaacagg tagagctggc ccggatcggt gtcgctggaa acggccacga
      301 tcatctgctt gccatcgcgg gtcgcgctgg tgatgtcgac gagctcgcct ggaaaggctt
      361 tcgacaacga cacgtacacc gccgcgtccg ggttcttctt gtcgaccagt tcgacatgcg
      421 gagcgccggc catcgtcacc acgccgacga tcgtcttctc gtcggatgcc ccgaggaaac
      481 cgtggatggc ggtgtccggg tcgcgataaa gcggatggaa cgcgttggtg gccggatcga
      541 ggatgccgaa ggccgacggc gtcttgccgt cgcccgccat cgcgtagatc cgcccgtccg
      601 ccgccgtgcc gacgacgcgc atggccggtt catccttgtt ggacttgtcg atgcgggtcc
      661 aggtgtcgcc atcgagccgg tacagctcgg tgtgttcgtt gtagccgagc ttggcgtcct
      721 tggtttccgt gcagttcgcg taccggggtt ggttggccgc atcgagcacc atctcgcagt
      781 cgtcgcgcgg agcccgtgcc agtacctggc gacgaccggt catcgtgtcg atcgccacca
      841 ctgcgttgcg gccgccggtc gagctgtcgt tgagctgcat caacgccttc gattcgtcgt
      901 tcggcaacgg atcgagcatc gacaggctct cgtggaaatg catgatcttg gactgtccct
      961 cggtcgcgct ggtgttgtac gacaccaggg tccgcgggcg accgccatcg gagtccatcg
     1021 cgtaccactc gcccgtgccg aaaggcacgg cgtagcttcc gaagttcttc gccgacgtgt
     1081 acatgatccg gttgtcgccg acccagtaga aactgccgac gctttcaccg ccggtcaggc
     1141 gcgtgatgcg gatcacctgc atgtccttca tgcgcagcac cgcgagtgac atcacgtcgc
     1201 cctgctgcat cgccagcgcc aggaagcgcc cgtcgggcga gatcttggtg gcggtgtatt
     1261 gcgggtggcg cacgaattcc tggatcggca ccgtatcgcc cgcgcgggcc agcatcggca
     1321 tggaccccag caacgcaacc atcagtacag tcttcgacaa cattttcctt ccccgtgtct
     1381 gggcggcccg gcgccgccat ctgtcgttca gtgcgccgcg gccgctgccg cgcccgtcgt
     1441 cgtcgctacg cctgccccgc cgccgatgtt gcggtggagg aacgcaagca gttgcgtgta
     1501 gaacgcgatg cgatgctcgt ccgtgtagaa gccgtggcct tccgtgcgga agtaaagcgt
     1561 ctccaccgga acgccagccg cgcgcaatgc cttttccatg cgctcactgt gggctatcgg
     1621 tgcacgctcg tccgcgccac cggctgcaag gaacaccgga gccttgatct ggccggccag
     1681 gttcaccggt gaaaccgcgc ccagttgggt cggatcaccg atccagccgc gcaggtacgc
     1741 cttgcccgaa tcgagcgcct gcgtatcgcc tttctggaac atcagcggca ggtcgtacac
     1801 accggcgtat ccagcggcgc acttgtacag cgacggctcc cgcgcgaggc ccatcagcgc
     1861 tgcataaccg ccataactcg ccccgtagat gcagatcctg gtgggatcgg cgtacccctg
     1921 gtcgactacc cagtgggtgg catcggtgat gtcgtcctgc agcgcacgac cccactggcg
     1981 cgcgccagcc tgggtgaaat cacgaccgta cccggaggat ccgcggaagt tgacctggac
     2041 cacggcgtag ccggccgcag caatcaactg cgtctcggta tcgaaacccc aatggtcggc
     2101 gacgaagaag gggccgccat gcgggatcac gaccgtcggc aggtgcttcc catcgctgcc
     2161 gcgcggaatg gtgaggaagc cgtggagcat tcggccgtcg cgcgccttga atgcgaaggg
     2221 gcgctgttcc gacatcgtcg ccgggtcctg ccattggcgc ggcgccagca ggtagccggc
     2281 cttcttgttg accgtatcga acagatagaa ctcaccagca tcacgatcgc tggagacgcg
     2341 taccagggcg agcgctccat ccgcggtctt ggaggtgatc tcaacgaggt cgcccgggaa
     2401 tgccgcctcg aggctgcgat acagcctggc ctcggggctt gcatcgtcaa agaactgcgt
     2461 acgcggtttc ccgtcgtcga tgaacgcacc taccggcgcc ggataaggcg ttcccatcga
     2521 gcggatgatg gcggccggat cgctatcgtc atcgcgcagg acggttttcc tggtgtcgtc
     2581 gcgcgtatcc agggccacga tcgaatccgg cccgttcgcc tgttcgacct gcaggtaggc
     2641 gatgttgccg tcagcggaga aaccaagcgg aacttcgatg tgtccggtcg aactctcgtc
     2701 gttgatttcc ctccagtcgg cggtgttggt ccagtcgacc ttggttttcg cgcgatagaa
     2761 cagcttatgg tcgttatcgg tttcataacc gaaggcgaag cgcactaccc cgtcggcgtc
     2821 ggtgacgtag tccgaattcc gcaccggcga acgcgctaca ggcacgcggc ggccgctata
     2881 gacgtccatt ttctcgaccc ggttgtaact gtcgcccgag aaaccggata ccgaaatcaa
     2941 tacgttgtga tcgtccgcag gaaggtcgtc gagcaggaag gccgcgacca tttccgtctt
     3001 cttggtctgg atgtgcgaac cggtgctcat cacgtcgacg tcctggccca ccaggatgtc
     3061 cttgccggtg ccatcggcgt tcatcgcgta caggttgccg gtgagctggg ggttgtcgag
     3121 ggcgccaaac ctgcgcgccg ttccgaacac cacgcgctgc gggttgaccc aggcgaaatc
     3181 ggaaatgtag gtgttcctgc cgatcccgaa accgcccgtt accttgttgt ctgcgcggcg
     3241 cacgatcacc aggaccgaac tgtcctcgcc gggaaccgat gcggcgtaat agtcgccatt
     3301 gggcgagagc ttgatctggt cgaagctatc gcgcttgacg aatgacgcca cgtcgacggc
     3361 tgtcgccgcc tgggctgcgc ctgcgaacag cagcgcggca gccaatacca gaattcgcat
     3421 gttttccttc ccctatccgc gcgcgcagcg ttgctccgcg cgtcggccgt tccgtcctga
     3481 cttcgccccc ggcgctcccg gtcgccgctc cacggccaac tccggactcg ccgggccagc
     3541 cacatcgcgt tcgatcacat ctccttcaat gcgaggcagc gttatccgct cctgccgcgc
     3601 cgctcatcgc gagcgcgcca ccgatgttgc
//
LOCUS       DATIMH010000076         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1750028, whole genome shotgun sequence.
ACCESSION   DATIMH010000076 DATIMH010000000
VERSION     DATIMH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750028"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VLI90_01305"
     CDS             <1..612
                     /locus_tag="VLI90_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HSV12866.1"
                     /translation="VLAGSAIGAVTPKSNRITGEVRDGDAIVMLASSGVQTNGLTLCR
                     QIAERMPQSYLKELSDGRTYGEALLDPSVIYVKFIAACQRENLRLRYAAHITGHGWRK
                     IMRLAEPFVYRIDRLAPALPVFEFIMRAGPVELKEAYGTFNMGAGFAAYVDAADAERC
                     VQIAKTAGYDAWVAGVVQKQGDRKAVEIAPLNMTFEGDTLNVR"
     gene            653..1033
                     /locus_tag="VLI90_01310"
     CDS             653..1033
                     /locus_tag="VLI90_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSV12867.1"
                     /translation="MAEELLAMARDLVKVTNVQSASVIDLSLPESLDSEEFDRLNESM
                     LKAVDGKSSGKWVLDLSAVDYMGSSVLGLMINIRQHIKTGGGKLALCGLSPRLTQIFH
                     TCCLEKLFTIAKTREEALRALGTR"
     gene            1179..1976
                     /gene="fdhD"
                     /locus_tag="VLI90_01315"
     CDS             1179..1976
                     /gene="fdhD"
                     /locus_tag="VLI90_01315"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HSV12868.1"
                     /translation="MNATAPDVLRYADGQICEFPDELAAEEPLEIRVRGRAISVTMRT
                     PGDDSDLAAGFLLTEGLIRHASDILNIQACERNEFSNVINVLLAPDVAVDFDRLTRHV
                     FASSSCGLCGKVTVEAVRGQFPPVQGDFAINAGVILKLPEIMRRTQATFDRTGGLHAA
                     AVFDERGELIVLREDVGRHNAVDKVIGHCLRSDRLPLDRHVLLVSGRSSFEIMQKALA
                     AGVAIVAAVSAPSSLAVEFARESGQTLIGFLREGRMNVYANPQRVRF"
BASE COUNT          409 a          659 c          803 g          484 t
ORIGIN      
        1 gtgctggctg gttcggcgat tggcgcggtg acgccgaagt ccaatcgcat caccggcgaa
       61 gtgcgtgacg gcgatgcgat cgtcatgctg gccagctcgg gggtgcagac gaacggcctg
      121 acgctctgcc ggcagatcgc cgagcggatg ccgcagagtt acctgaagga gctgagtgac
      181 ggccgaacct acggcgaagc gctgctcgat ccatccgtca tttacgtgaa gttcatcgcg
      241 gcctgccagc gcgagaattt gcggctgcgt tacgcggctc acatcactgg gcatgggtgg
      301 cgcaagatca tgcggctggc cgagccgttc gtgtaccgca tcgatcgcct cgcgccggcg
      361 ctgccggttt tcgaattcat catgcgtgcc ggtccggtcg aattaaaaga agcgtacggc
      421 accttcaaca tgggcgcggg cttcgcggcg tatgttgatg cggccgacgc ggagcgatgc
      481 gtgcagatcg ccaagacggc ggggtatgac gcgtgggtcg ctggcgtggt gcagaagcag
      541 ggcgatcgca aggcggtgga gatcgcgccg ctgaacatga ccttcgaagg tgatacctta
      601 aacgtgaggt agcgattcgt cctttgttag cccgcggaat tatggtgtaa tggtggccga
      661 ggagctgttg gcgatggcga gagacctggt caaggtgacg aacgtgcagt cggcgagcgt
      721 catcgatttg tcgctgccgg agtcgctgga ctccgaggaa ttcgatcgac tgaacgagtc
      781 gatgttgaaa gccgtcgatg gcaagtccag cggcaaatgg gtgctggatt tgtcggcggt
      841 ggactacatg ggcagctcgg tgctggggct aatgattaac atccgccagc acatcaaaac
      901 cggcgggggc aaactggcgc tgtgcgggtt gtcgccgcgg ttgacgcaga tttttcatac
      961 ctgctgtttg gaaaagttgt tcacgatcgc caagacgcgc gaggaggcgc tgcgggcgct
     1021 ggggacgcgg tgatttctga ttgattggcg ccgtcccgtg gcagagccac gggcttttgt
     1081 tttgcaagtg gtatttctat aaaagcccat ggctctgccg tgggactatg cgtttttccc
     1141 cttattgaaa tcgcccgctg cgcgctacgc tggcgcgcat gaatgccacc gcgcctgatg
     1201 tgctgcgtta cgccgacggt cagatttgcg aattccccga cgaactcgcg gcggaagaac
     1261 cactcgagat ccgcgtgcgc ggccgcgcca tcagcgtgac gatgcgcacg cccggcgatg
     1321 actcggactt ggccgctggg tttttgttga ccgaaggttt gatccgccac gcgagtgaca
     1381 tcctgaacat ccaagcctgc gagcgcaacg aattcagcaa cgtgatcaac gtgctgctgg
     1441 cgccggatgt cgcggtcgat ttcgatcggc tgacgcggca cgtgttcgcc tcgagcagct
     1501 gcgggttgtg cggcaaggtg accgtcgaag cggtccgtgg gcagttcccg ccggtgcagg
     1561 gcgattttgc gatcaacgcc ggcgttatcc tgaaactccc cgagatcatg cggcggacgc
     1621 aggcgacgtt cgaccgcacc ggcgggctgc acgcggcggc ggtgttcgat gagcgcgggg
     1681 agttgatcgt gctgcgcgaa gatgtcggcc ggcataacgc ggtggacaag gtgatcgggc
     1741 attgcctgcg gtcggatcga ctgccgctcg atcggcacgt gctgctggtc agcgggcggt
     1801 catcttttga gatcatgcag aaggcgctgg ctgcgggggt ggcgatcgtc gcggcagtgt
     1861 cggcgccgtc gagccttgcc gtggagttcg cgcgagagtc ggggcagaca ttaattggat
     1921 ttctgcgcga ggggcgaatg aacgtttacg cgaatccaca gcgcgtgcgg ttctgatctc
     1981 actgggataa acgctcacgt gacacgggcc tccggccggt gcttggtgcg ccgtgatttg
     2041 gcgatggcac gatcacgaat gaccctcgct tcacgcacgg gccggaggcc cgtgtcacgg
     2101 tgagcgcctt gggtgtccat tcagtcgtta ttttccctcg aaacttttcc gagtcctgcc
     2161 cgtccaaccc tgccgacgcg atgctcgtcg tgagtgtcgc ttccacacct ctcgctttca
     2221 ccggaggcgt tatgcgtcgg cgtatcctcg tcctggcggc caccgcttgc ttcatgggca
     2281 taaacatggc tccaacattg gcgacggatg cgccgactac ggcaccgtcg actcggcccg
     2341 cgccgaagga actga
//
LOCUS       DATIMH010000077         5376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4059126, whole genome shotgun sequence.
ACCESSION   DATIMH010000077 DATIMH010000000
VERSION     DATIMH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4059126"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..830)
                     /locus_tag="VLI90_01320"
     CDS             complement(378..830)
                     /locus_tag="VLI90_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HSV12869.1"
                     /translation="MDPFLRAAIDEARQGLAEGGLPIGSVLVRNGEIIGRGHNRRVQH
                     GDPMAHAEIDCLTQAGRQSTYRDTVLYSTLMPCYLCTGAVIQFGVPKVIVGEATTFPG
                     GEGKWGKSPDFMRANGVQVINLHDPECIEMMTTFIREHPELWNEDIGK"
     gene            1069..1455
                     /locus_tag="VLI90_01325"
     CDS             1069..1455
                     /locus_tag="VLI90_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12870.1"
                     /translation="MIGKLSVAVLACGLGFGILGCQDQNSGMPNNNDNTYPSSTDNNT
                     GSVGTTGTYDNNGTYHSGSGTYNNTNGTGAFGSWNSDSGTPSGNAHGGMNSGSGWDRG
                     SNNRESTGGAMNNTGSMSKEQPSTQP"
     gene            complement(1674..2384)
                     /gene="rsmG"
                     /locus_tag="VLI90_01330"
     CDS             complement(1674..2384)
                     /gene="rsmG"
                     /locus_tag="VLI90_01330"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HSV12871.1"
                     /translation="MNPLWTDIAARADIALGEQQHALLSRYLDLLLEANARMNLTRIT
                     DRAAAELQHVADSFTLLPYLPKTPHALADVGSGGGVPGIPLAIARPDARVTLIEATKK
                     KAVFLKETVAALQLSNVTIVDQRAEDVARGDLREAFDIAIARAVATMDWLAEWCLPLV
                     SKGGKMLAMKGPKVNEELPTAEPIIRLLGGMPPIVHPVDLPGTEHRVIVEITKGRATS
                     PRYPRPATVAKGKPLSPA"
     gene            complement(2669..>5376)
                     /locus_tag="VLI90_01335"
     CDS             complement(2669..>5376)
                     /locus_tag="VLI90_01335"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSV12872.1"
                     /translation="KVKLALGSKRHWCTLVAALFGICLATPGVSRGADDAASSRLTYD
                     KQCLTLDGKDYFLFSGAFHYFRCPKELWHDRFRTLKAAGFNTVETYVAWDWSEPTPPA
                     NVDDYSKLDLTDLHDWLAMAIDEFGFNVYIRPGPYICAEWDGGGYPQWFVTQRPANFA
                     GKQFTRGDDPAYLAWCKHWYTAVAKVAAPFQVTHRPAGKAGIILWQIENEYNYAAQPA
                     DVKLHQLQFLAHTSRDLGIDVPLTTCMTSDSPAFRKDDFLLKNVVETRNTYPKYSVQS
                     EGKDILFLDKYQPEKFRMVTELQGGWFSQVGGLLSEQQGFDASHINHVTMFAWENGFT
                     ATNYYMGFGGTNLGDWAASDITTSYDYDAPVREPGGVTARYFAVQALGQFLHDHASQL
                     VRATLENVEIKNKTDDDVTITVRKAPDGSRFVFVRTEERDGIRRGTATLQTTDDSPKQ
                     IIVGYELGPFGSKVLYLPIGANSDFDGQWYPKKVDGPKRPDLSELPAPVKITEARVKA
                     DPGPSAWQPIKPGQTEEAAGIFNRGFVFYRATVPALPQGQKTTLVARVPGRDWAGFWI
                     NGKRIGADAKDGGQLLGSSNTGEALLGLYENYGRPNGGAELDRGCGITDVKLIPITDV
                     TRTIDGWRVKHDLTGKKHPEEVSPKTDDSAWPTADITADDGDMKPGETAVYRAWMELT
                     DAELASPKTLVLGRVDEIGTVVVNGKTIGECRDWQVAQRFDVGSNLHAGKNLIAVIVN
                     NQSGVGGLARGASLESNGPAIATQWEISNQTAGATGKWWENDLDDSSWQPAKVSGDVQ
                     PGATASPLTWYRMKFDLPAPKPHIWAPLKLRLDAAGNGFLYLNGHALGRWWQVGPQHD
                     FYLPECWLNTGEGKTNVLTISLRPTDGVGVKSAEVVPYENQAEMR"
BASE COUNT          896 a         1654 c         1748 g         1078 t
ORIGIN      
        1 cagcgctggc tggcgacgca tgggacgttc gagcacgaag gcgacggcgc ggggcgtggc
       61 gtggcggaca tcacgcggac gtatgtgcgg cagcaggcgc gggcgaaatt tatttgttta
      121 tcctgagcgg cggcgtactc gcgggagtcg aaggatttgg ccgaagcaga tgaacgtttg
      181 gatccgagga tggtgcggcc gtcgcttgcg gtttggcaga ccagtgaccg cggcttgtcc
      241 gtctgcgaag ccgcaagcgg ctgccgcgcc cgtttgcgcg agtgatgctt cactgactcc
      301 cggctatgtc cttcgactcc ggcggagtac cgtctgactt cgacgggctc agtcgagtcg
      361 ccctcaggat aaagtcttca cttcccaata tcctcattcc acaactccgg atgctcgcgg
      421 atgaacgttg tcatcatctc gatgcactcg ggatcgtgca ggttgatcac ctggacgccg
      481 ttggcgcgca tgaagtcggg gcttttgccc cacttgcctt cgccgccggg gaatgtggtg
      541 gcttctccta cgatcacctt gggtacgccg aattgaatca ccgcgccggt gcacaggtag
      601 cacggcatca gcgtgctgta gaggaccgtg tccctgtacg tgctctggcg gccggcttgg
      661 gtgaggcagt cgatctcggc gtgggccatg ggatcaccgt gctgcacgcg gcggttgtgc
      721 ccgcggccga tgatctcgcc gttgcggacc aagaccgaac cgatgggaag gccgccttca
      781 gccaggcctt ggcgggcttc gtcgatagcg gcacgcagga aggggtccat tcgatgttct
      841 ccgatttgat gcggtggtgc gaccttcgaa tgtaccggcg gagtcgcctg cggggcaact
      901 tcaaaggctg tggctggcgg cagcgcgagc ccgcgcgtgg aatgcaaatt gcaattcgtt
      961 gatccgattc agtgagccgg ccagaacgac gcaagcgatt ttgtcgcacg tgcggtcgct
     1021 ggtttttcgt ctacctcgtt ggcggccctt ggccgcagga gatcgatcat gattggcaaa
     1081 ctttccgtcg ctgtcctcgc ttgcgggctc ggctttggca tcctcggttg ccaggaccag
     1141 aacagcggca tgccgaacaa caacgacaat acgtacccgt cctcaaccga taacaacacc
     1201 ggatccgtcg gcacgaccgg gacgtacgac aacaacggca cctaccacag cggcagcggg
     1261 acatacaaca acacgaacgg caccggagct ttcggcagtt ggaacagcga cagcggcacg
     1321 cccagcggca acgcccacgg cgggatgaac tccggcagcg gttgggatcg cggcagcaac
     1381 aaccgcgaat cgaccggcgg cgcgatgaac aacaccggtt cgatgtccaa ggaacagccg
     1441 agcacgcagc cgtaaggcga ggccccgcgc gcattggcgc gcgctgcgtg atgacaattt
     1501 gtttgacagc ttgcggcttc gcgattcgga cggtgtttgt ttcgcgaagc cgcgagcgcg
     1561 cgcttcgacg tattcattta catcgtgcgt agcgattggt tgtgtagcgg tagccccgca
     1621 gggcacgtgt ttttcgtttc gaacgagaaa tacgtgcccc tgcggggcta ccgctacgcg
     1681 ggggagagtg gctttccctt ggcgaccgtc gcgggtctgg gatagcgcgg gctcgtcgcg
     1741 cgccctttgg tgatctccac gatcacgcgg tgctcggtgc cggggagatc gaccggatgc
     1801 acgatcggcg gcataccccc gaggaggcgg atgatcggct ccgccgtggg caattcttcg
     1861 ttcaccttcg ggcctttcat cgcgagcatc ttgccgcctt tgctgaccag cggcaggcac
     1921 cactcggcga gccaatccat cgtcgcgacc gcgcgggcga tcgcgatgtc gaaggcttct
     1981 cgaagatcgc cgcgggccac gtcttcggca cgttgatcga cgatcgtcac gttcgacagc
     2041 tgtaaagcgg cgacggtttc tttcagaaat accgcttttt tcttagtcgc ttcgatgagc
     2101 gtcacgcggg cgtccggccg ggcgatggcc agcgggatgc cgggtacacc cccgccgctg
     2161 ccgacgtcgg cgagcgcgtg cggtgtcttg ggcaaatacg gcagcagcgt gaacgagtcc
     2221 gcgacgtgct gcagttcggc agcggcgcga tcggtgatgc gcgtgaggtt catgcgggcg
     2281 ttcgcctcga gtagcaggtc gaggtaacgc gacagcagcg catgctgctg ttcgccaagc
     2341 gcgatgtcgg cgcgcgcggc tatgtcggtc cagagtgggt tcatggaaag tttcggcacc
     2401 ggggtcgggg gacaattcag atttgtcatc ctgagcgaag cacgtactcg tgcgcagtcg
     2461 aaggatctag ccgagtagtc ggtgaacgtt cagttgcgga aaacgtgcgg cagtggctta
     2521 cggtttcgca gatcagtgac cgcggcttgt ccgtctgcga aaccgcaagc gactgctgca
     2581 acatttgggc aaatgaacgt tcaccgagtt ctcggctaga tccttcggct ccggcgagta
     2641 cgcctgcgct caggatgaca actgcgcgtc aacgcatctc cgcctggttc tcgtatggca
     2701 cgacctccgc gctcttcacg ccgactccat ccgtcggccg cagggagatc gtcagcacgt
     2761 tcgtcttgcc ctcgcctgtg ttcagccagc actcgggcag atagaaatcg tgctgcgggc
     2821 cgacctgcca ccagcggccg agcgcatggc cgttgagata tagaaatccg ttgcctgcgg
     2881 catcgagccg cagcttcagc ggggcccaga tgtgcggctt gggcgccggc agatcgaact
     2941 tcatgcgata ccacgtcagc ggcgacgccg tcgcgccggg ctgcacgtcg ccgctcactt
     3001 ttgcgggctg ccacgatgag tcgtccagat cgttctccca ccacttgccg gtcgcaccgg
     3061 cggtctgatt ggagatctcc cactgggtcg caatcgccgg gccatttgat tccagggatg
     3121 ccccgcgcgc caacccaccg acgccggatt ggttgttcac gattaccgct atcaggttct
     3181 tacccgcgtg caggttgctg ccgacgtcga agcgctgggc gacctgccag tcgcggcatt
     3241 cgccgatcgt cttgccgttg acgacgaccg tgccgatttc atcgacccgg cccagcacca
     3301 gcgtcttggg cgacgccaat tccgcatcgg tcagttccat ccacgcgcga tacaccgcgg
     3361 tttcaccggg cttcatatcg ccgtcgtcgg cggtgatgtc ggcggtcggc caggccgaat
     3421 cgtcggtctt gggcgagact tcttcggggt gcttcttgcc ggtcaggtcg tgcttcacgc
     3481 gccagccgtc gatcgttcgc gtgacgtcgg tgatggggat gagcttcaca tcggtgatgc
     3541 cgcaaccgcg gtcgagttcc gcgccaccgt tgggccggcc gtagttttcg tacaggccca
     3601 gcagcgcctc gccggtgttg gatgaaccga gcaattgccc gccgtctttg gcgtcggcgc
     3661 cgatgcgctt gccgttgatc cagaaaccgg cccagtcgcg gccgggcacg cgcgcgacca
     3721 gcgtcgtctt ttgtccctgc ggcagcgctg ggaccgtggc gcgatagaag acgaacccgc
     3781 ggttgaagat gcccgcggct tcctcggtct gccccggttt gattggctgc cacgccgacg
     3841 gtcccgggtc ggccttcacc cgcgcttcgg tgatcttcac cggcgcgggc agctcgctca
     3901 ggtcagggcg cttcggcccg tcaacttttt tcggatacca ctgcccgtca aaatcgctgt
     3961 tggcgccgat cggcagatac agcaccttcg atccgaacgg cccaagctcg tacccgacga
     4021 tgatctgttt cggcgaatca tcggtcgttt gcagcgtcgc ggtcccgcgg cggatgccgt
     4081 cgcgctcttc ggtgcgcacg aagacgaacc ggctgccatc gggcgccttg cgcacggtga
     4141 tggtcacgtc gtcatcggtc ttgttcttga tctcgacgtt ttccaaggtc gcgcgcacca
     4201 gctggctcgc gtgatcgtgc aggaactggc ccagcgcctg cacggcgaag tagcgggcgg
     4261 tcacgccgcc gggctcacgc accggggcat cgtagtcgta gctggtggtg atgtcggatg
     4321 cggcccagtc gccgaggttc gtcccgccga aacccatgta gtaattggtg gcggtgaacc
     4381 cgttctccca ggcgaacatc gtgacgtgat tgatgtgcga cgcgtcaaaa ccctgctgtt
     4441 cgctgagcaa tccgccgact tgggaaaacc acccgccctg cagttccgtc accatgcgga
     4501 acttttcggg ctgatatttg tcgaggaaca ggatgtcctt gccctcgctc tgcacgctgt
     4561 acttcgggta cgtgttgcgc gtttcgacga cgttcttaag gaggaagtca tccttgcgaa
     4621 acgcgggtga gtcgctggtc atgcaggtcg tcagcggcac gtcgatgccc aggtcgcgcg
     4681 acgtgtgcgc caggaactgc agctgatgca gcttcacgtc cgccggttga gcggcgtagt
     4741 tgtattcgtt ctcgatctgc caaaggatga tgcccgcctt gcccgcgggg cgatgggtca
     4801 cctggaacgg cgccgccacc ttggcgacgg cggtgtacca gtgcttgcac caggcgagat
     4861 acgccggatc atcaccgcgc gtgaactgct tgccggcgaa gttggccggc cgctgcgtga
     4921 cgaaccactg cgggtatccc ccgccgtccc attcggcgca gatataaggc cccgggcgga
     4981 tgtagacgtt gaagccgaac tcgtcgatcg ccatcgccag ccaatcgtgc aggtcggtca
     5041 ggtcgagctt cgagtagtca tcaacattcg ccggcggcgt gggttcgctc cagtcccagg
     5101 cgacgtaggt ttcgaccgtg ttgaaccccg cggccttgag cgtgcggaag cggtcgtgcc
     5161 acaactcttt ggggcagcgg aaatagtgga acgcgccgct gaacaggaag tagtcttttc
     5221 cgtccagcgt gaggcattgc ttgtcgtaag tcagccgtga cgaggcggcg tcatccgcgc
     5281 cgcggctgac gccgggcgtc gccaggcaga tgccgaacag cgcggcgacg agggtgcacc
     5341 aatgtcgttt cgaaccgagc gcaagtttca ctttgt
//
LOCUS       DATIMH010000078         7984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_17472, whole genome shotgun sequence.
ACCESSION   DATIMH010000078 DATIMH010000000
VERSION     DATIMH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17472"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="VLI90_01340"
     CDS             <1..1793
                     /locus_tag="VLI90_01340"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HSV12873.1"
                     /translation="ITGDFNKDGKIDIAASNNGSNDVAIYLGKGNGQFNNPVFYPVGM
                     AVRGIASGDFNKDGNVDLVTANKDDGTVSVLLGNSNGTFKTQKTYFAGTGAWAVVDVD
                     FNGDGKLDLAVADSASANVQILLGKGDGTFGTATPVPVPFYPMAIRTLDVNNDGKADL
                     VTANQLSGNVSVLLGKGNGTFNSPLNTTVGSSPDSLAFGDFNNDGKKDLAVTDFQDDT
                     VTVLLGKGNGTFQTPVPYHAGDTPQGLATADLNKDGKLDLIVSNRSDGVNDPSGNVQV
                     FLGVGDGTFLPPFAFTAHKSPVGTAFADFNGDGRLDLAVANFNSDDVSVLLNATVPIT
                     TGNISGSVFSDTNQNGKKDSGETALSGVTIFIDANKNGVQDAGERGVKSDSNGNWSFT
                     GLTAGTYRIRSERGASVIHTLPAGSAAFYDVTVTPGLTAGGKVFGIAMASPSSNLFRS
                     IDAGNTTANAFFDNAGHTWTADTGVSGGTTVSSAFEVANTDDDALYYAYRQGANFTYT
                     ISVPNGTYTVDLLFVEPTKTAAGQRKFSVKSQGSTVLSNFDIFAAAGGAKRAVTKSFN
                     AMVTNGKLVLQFIGSMDLALVSAIDVRALS"
     gene            complement(1931..3133)
                     /locus_tag="VLI90_01345"
     CDS             complement(1931..3133)
                     /locus_tag="VLI90_01345"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HSV12874.1"
                     /translation="MQKLWQQLAIATNWPVLAAVVVLSSLGAISIWADAADAGAKQLL
                     YLGVAFACMVAFQAVSYMVIARWAWPFYIASLLLVAYTVAGSKMTLPFVHNTKGVYAW
                     INLGPMSLEPSELVKIGFILVTARYLRYRSNYRTVIGLLPPFALALVPIILILKQPDL
                     GVAALFLPTLLAMLFVAGARVKHMVLILAMGVMFMPIFWFAGHRYTDVTEKIRDPAKE
                     VPVLRHLPTLMHEYQRDRVTAIFAHDPKSSGGLNYQQDHALMAFGSGGITGKGIGEVP
                     VGRHVPEAHNDMVFSLIGEQFGFFGAAAVLGAYLVLFAAGIEIAAATKEPLGRLIAVG
                     TVAMLACQASINLMVCLRLMPVTGVTLPFVSYGGSSLIASFMAAGLLLNVGQNRPLMI
                     GKQAFEYD"
     gene            complement(3151..4023)
                     /locus_tag="VLI90_01350"
     CDS             complement(3151..4023)
                     /locus_tag="VLI90_01350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HSV12875.1"
                     /translation="MIRRIALGTVGRTPVGLRSWVYRHPRIWHPLARVIGRLVPHDQI
                     TILDIRVGPNAGMKLAANRATPRYYWLDGNYEADVVRQLREWVRPGMVVADVGAHVGF
                     MAMYLAKQVGPGGAVLSFEPSPAAMAQLRQNVEINRLTQVTPMQIALSDAAGQARFIV
                     RPYSTTSGLARGGDSDGNSIEVRMERLDDVVFAAGGTQRLDVVKIDVEGSEAAVLRGA
                     ARVLAELRPNLLIEVHNAAALADCLSQLRAARYEVIALHPDPYYEVVLRDPAAAQGGD
                     AAAFAINHLRCLPR"
     gene            complement(4032..4799)
                     /locus_tag="VLI90_01355"
     CDS             complement(4032..4799)
                     /locus_tag="VLI90_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12876.1"
                     /translation="MTTSIPGPTSVPPPPPPPPAAPRPLDRRTAARLWAEPRVRIWLV
                     ITGALLLLAIYFSIARIQEWKLDRDLVRSGISLQATVGVAGVGRGGYAIPPGTPVELT
                     FDFRGEPQHVSGYLEDQTTPVTGQKINIRIDPDDPQRWTDLAEPRPLAGYLIGPALMV
                     AIVPITLVTAWMIRRRFARTWAGGAVQAAVVVDRLQSAMAPMAQTIRCRFRDKPERRL
                     LVVYLPRSFAKVQRGDVVWVLVPARTLRPALAAGWVA"
     gene            complement(4825..7215)
                     /locus_tag="VLI90_01360"
     CDS             complement(4825..7215)
                     /locus_tag="VLI90_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSV12877.1"
                     /translation="MFERRLKIFLLILLLITSILLTRAIHVQVFAHQYWTNRAAGLLQ
                     TSQTTATTRGRILDITGKELAIEVASTDACVDYRAITEPPDPAWVNAIANTRMRNRFG
                     ADLRKMPAEQRATLKDLEVRKVQADVETMWATLAELYQPDDNDPVLADGTFDRRAAVD
                     EIRHEIQRRVEMRKRWVWYRNFQLNRTKASDAPRWQRWLTGGGEGPDIDSFAVTVEDE
                     KQPHVVLRNIDLDTQNFLGKRMEQFPGLSLQASTHRRYPLEEVACHTLGRMARVSASD
                     VADAKADGWDETRAYLPNDQIGRDGIESLCEPLLRGARGRIERRVGDGLIVKQEDFVP
                     GQDVRLSIDADLQREVQQMLKRVKMTLFHEDPARRKEVELNMHAAAVVIDVATGELRV
                     MASNPDFNANDLDTRYAALMADRLNNPLKNRATMDEFEPGSTVKPMLGLGAITQGVLG
                     PTEGIECTGYLILDYNGRKVRQSIGRCWVASEYADRLAQLHMSVAHHPIPPEYPHRGH
                     DGNPDGWLSYSDAIERSCNVFFETVADRLGPAGIDHWYRAFGFGQRTGIGIPERPGRL
                     PGSVAMIGAMNRMTNCFAGIGQSQVWATPLQIANEAATIARGGVWMRPRLLTGDTQTR
                     LDAVRKRNNDGRPDRVDLHLSPEGLRQAKIGMVAVVNDPAGTGQTAYRDDMVIAAKTG
                     SAEGTHLWLDSKGAIDQNGKPVREPLPPANDTTPNTGTEWYRSSDPDGKHFVHAWFMG
                     FAPAENPQIAFCVFIEYAGAGGGTAAGPVVSEILEACVKHGYLHAHPPETPFVLAH"
     gene            complement(7393..7944)
                     /gene="mreD"
                     /locus_tag="VLI90_01365"
     CDS             complement(7393..7944)
                     /gene="mreD"
                     /locus_tag="VLI90_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HSV12878.1"
                     /translation="MRWFAYFILAYVMLGLQLGLGAHVQYRGISPNLVLLVVVFISLN
                     APRDEALLGSFLLGAMQDLVTLQPMGLYAFSYGIVSMLVTTVGQLAYREHPLTQLFMT
                     LIGGTITSTIVMIHGWVHPLGPARTDGIHVIPAVRQSPRALLVCVVYTAILAPIIIGI
                     LQRMQRMFGFQPSGRRRSRGGER"
BASE COUNT         1516 a         2667 c         2452 g         1349 t
ORIGIN      
        1 tcatcaccgg cgacttcaac aaggacggca agatcgacat cgccgcctcg aacaacggta
       61 gtaatgacgt cgcgatctac ctcggcaaag gcaacgggca gttcaacaat cccgtcttct
      121 atccggtcgg catggccgtg cgcggcattg ccagcggtga cttcaacaag gacggcaatg
      181 tcgatctcgt caccgccaac aaggacgacg gcaccgtgag cgtgctgctg ggcaatagca
      241 acggcacgtt caaaacgcag aagacatact ttgccggcac cggcgcgtgg gcggtggttg
      301 acgtggattt caatggcgat ggaaagctgg acttggccgt cgcggacagt gccagcgcca
      361 atgtgcagat cctgctcggc aaaggcgacg gcaccttcgg cacggccaca ccggtgccgg
      421 tgccgtttta tccgatggca attcgcacgc tggatgtcaa caacgacggc aaggccgacc
      481 tcgtcacggc caaccagttg agcggcaacg tcagcgtgtt gctcggcaag ggcaacggca
      541 cgttcaactc accgctcaac acgacggtcg gttcgagccc ggacagtctc gcgttcggcg
      601 attttaacaa cgacgggaag aaggacctcg ccgtcaccga cttccaggat gacacggtga
      661 cggtcctcct cggcaaaggc aacggtacgt tccaaacgcc ggttccatat catgccggcg
      721 acacgccgca ggggctggca acggccgacc tgaataaaga cggcaagttg gatttgatcg
      781 tctccaatcg cagcgatgga gtgaacgatc caagcgggaa cgtgcaagtc ttcctcggcg
      841 tcggcgacgg aacgttcctg ccgccgttcg ccttcaccgc ccataagagc ccggtcggaa
      901 cggcattcgc cgacttcaac ggtgacggcc ggctggatct ggctgtcgcc aacttcaata
      961 gcgacgacgt cagcgtgctg cttaacgcca ccgtgccgat caccaccggc aacatctccg
     1021 gcagcgtctt cagcgacacc aatcaaaacg gcaagaaaga cagcggcgag accgcgctga
     1081 gcggtgtgac gatctttatc gacgccaaca agaacggcgt gcaggacgcc ggcgaacgcg
     1141 gcgtcaagag cgacagcaat ggcaactgga gcttcaccgg tttgaccgcc ggcacgtatc
     1201 gcatccgctc cgaacgcggc gcaagcgtca tccacacgct gcccgccggc agcgccgcgt
     1261 tctatgacgt caccgtcacg ccgggtctca cggccggcgg aaaagtgttc ggcatcgcga
     1321 tggccagtcc atcgtcgaat ctcttccgat cgatcgacgc gggcaacaca acagcgaacg
     1381 cgttcttcga caacgccggt cacacttgga ctgccgacac cggcgtctcc ggcgggacca
     1441 cggtttccag cgcgttcgaa gtcgccaaca cagacgacga tgcgctctat tacgcctacc
     1501 gccagggcgc gaacttcacc tacacgatca gcgtgcccaa tgggacgtac acggtggacc
     1561 tgctgtttgt tgagccgacc aagaccgccg cggggcagcg gaagttcagc gtgaagtcgc
     1621 agggcagcac ggtgctgagc aacttcgaca tcttcgccgc cgccggcggc gccaagcgcg
     1681 cggtgaccaa gtcgtttaac gcgatggtca ccaacggcaa gctggtgttg cagttcatcg
     1741 gcagtatgga tctagcgctg gtgtcggcga tcgacgtcag agccttgtcc tgagcgcagc
     1801 acgtactcgt gcgcagttga aggatatagc tggaggtaag tgcgctgttg caagcgcgag
     1861 acattcacta acgccgaggc catatccttc gactccgcac gggcacgtgc tgcgctcagg
     1921 ataaagtgcg tcaatcatac tcaaacgcct gcttcccgat catcagcggg cgattctgcc
     1981 cgacgttcag cagcaacccc gcggccatga agctggcgat caggctgctg ccgccgtagc
     2041 tgacgaacgg cagcgtcacg cccgtcaccg gcatcaatcg cagacaaacc atcaggttga
     2101 tcgacgcttg gcacgcgagc atcgcaaccg tgccgacggc gatcaaccgg cccagcggtt
     2161 ccttggtcgc cgccgcgatt tcaatccccg cggcgaacag aaccagatac gcccccagca
     2221 ccgcggcggc gccgaagaag ccgaactgct cgccgatcag cgagaagacc atgtcgttgt
     2281 gcgcctcggg cacgtgccgg cccaccggca cttcgccgat gcccttgccg gtgatcccac
     2341 cggaaccgaa ggccatcagc gcgtggtcct gctggtaatt caatccgccg gacgatttgg
     2401 gatcgtgcgc gaagatcgcg gtcacgcggt cgcgctggta ctcatgcatc agcgtcggca
     2461 ggtgccgcag cactgggact tccttggctg ggtcgcgaat cttctccgtc acgtccgtat
     2521 accgatgccc ggcgaaccag aagatcggca tgaacatcac gcccatcgcc aggatcagca
     2581 ccatgtgctt cacccgcgcg ccggcgacga acaacatcgc cagcagcgtc ggcaggaaca
     2641 acgcggcgac gccgaggtcc ggctgcttca ggatcaggat gatcggcacc agcgccagcg
     2701 cgaacggcgg cagcaggccg atcaccgtgc gatagttcga tcgatagcgc aggtagcgcg
     2761 cggtgacgag gatgaacccg atcttcacca gctccgacgg ctcgaggctc atcggcccca
     2821 ggttgatcca cgcgtacacg cccttggtgt tgtgtacgaa cggcagcgtc atcttcgacc
     2881 ccgccacggt gtaggcgacg agcagcaggc tggcgatgta aaatggccac gcccaccgcg
     2941 cgatgaccat gtagctcacc gcctgaaacg ccaccatgca cgcgaacgcg acgccgaggt
     3001 aaagcaactg cttcgccccc gcgtccgcgg cgtcggccca gatgctgatc gcgccgagcg
     3061 acgaaagcac gaccaccgcc gccagcaccg gccagttggt tgcaatcgcg agttgttgcc
     3121 aaagcttttg cattgaacag acagccgatc ctatcgcggc aagcagcgca ggtggttgat
     3181 cgcgaacgcc gcagcgtcac cgccttgcgc cgcggcggga tcgcgcagga cgacttcgta
     3241 gtaaggatca gggtgcagcg cgatcacctc gtagcgcgcg gcgcgcagct gcgaaaggca
     3301 atccgccagc gcggccgcat tgtgcacctc gatcagcaga ttcggccgca actccgccag
     3361 cacgcgcgcg gcgccgcgca gcacggcggc ttcactgcct tcgacgtcga tcttgaccac
     3421 gtcgagccgt tgagtgccac cggcggcgaa gacgacgtcg tccagccgct ccatccgaac
     3481 ttcaatcgaa ttgccgtcgc tgtcgccgcc gcgggccaga ccactcgtgg ttgaatacgg
     3541 tcgaacgatg aagcgggcct gcccggcagc gtcggacaag gcgatctgca tcggtgtcac
     3601 ctgcgtcagc cggttgatct cgacgttctg ccgcagctgc gccattgccg caggactcgg
     3661 ctcaaagctc aacaccgctc cgcccggccc gacttgcttt gccagataca tcgccatgaa
     3721 accgacgtgc gcgccaacgt cggcgacgac catcccggga cggacccact cgcgcaactg
     3781 gcgcacgacg tccgcttcat aatttccatc gagccagtaa taccgtggcg tcgcgcggtt
     3841 ggccgccagc ttcatgccag cgttgggacc gacgcggatg tcgagaatgg tgatttggtc
     3901 gtgcggcacc agccggccga tcacgcgagc cagcggatgc cagatccgcg gatgccggta
     3961 gacccaggac cgcaggccca ccggcgtgcg gccgacagtg cccagcgcga ttcgcctgat
     4021 catcgcgctc atcacgccac ccaacccgcc gccagcgcgg gccggagcgt tcgcgccggg
     4081 acgagcaccc agacgacatc gccgcgctga accttggcga acgatcgcgg caggtagacg
     4141 accagcagcc gtcgctcggg cttgtcgcga aagcgacagc ggatcgtctg cgccatcggc
     4201 gccatggcgg attgaaggcg atccaccacg acggcagctt gaaccgcgcc gccggcccag
     4261 gtgcgagcaa accggcgtcg aatcatccac gccgtcacca gcgtgatcgg cacaatcgcc
     4321 accatcagcg ccgggccgat gagatagccc gccagcgggc gcggttccgc caggtcagtc
     4381 catcgctggg ggtcgtcggg gtcgatgcgg atgttgatct tttgccccgt gaccggggtg
     4441 gtttggtctt cgaggtagcc gctgacgtgc tgcggttcac cgcggaaatc gaaggttagt
     4501 tcaacaggtg tgccgggcgg gatcgcatat ccgccgcgac cgacaccggc gacgccgacc
     4561 gtcgcttgca gcgaaatgcc gctgcgcacc aggtcgcggt cgagcttcca ctcctgaatg
     4621 cgggcgatgg agaaataaat tgccagcaac agcagagcac cggtgatcac gagccagatg
     4681 cggacgcgcg gctccgccca gaggcgcgcg gcggttcgcc gatccaacgg ccgcggcgca
     4741 gccggtggcg gcggcggcgg cggcggtaca gacgttggac ccggaatcga tgtggtcaat
     4801 ttcgtctcgc gatgcgggca aacgctaatg tgccagaacg aacggcgtct ccggcggatg
     4861 cgcgtgaagg tatccgtgct tcacgcaagc ctcaaggatt tcgctcacca ccggccctgc
     4921 cgcggtgcca ccgccggcgc cggcgtattc gatgaagacg caaaacgcga tctgcgggtt
     4981 ctccgccggc gcgaatccca tgaaccacgc gtgcacgaaa tgcttgccat ccggatcgct
     5041 ggatcgatac cactcggtcc cggtattcgg cgtcgtgtcg ttcgccggcg gcagcggttc
     5101 gcgcacgggc ttgccgttct ggtcgatcgc gcccttcgag tccagccaca ggtgcgtgcc
     5161 ttcagcgctg ccggtcttgg cggcgatgac catgtcatcg cgatacgccg tctgccccgt
     5221 cccggccggg tcgttcacca ccgcgaccat gccgatcttc gcctgccgca ggccctcggg
     5281 gctcaggtgc agatcgaccc ggtcaggccg accatcgttg ttgcgcttcc gcaccgcatc
     5341 gaggcgcgtt tgcgtgtcac cggtcagcaa ccgcgggcgc atccacacac cgccgcgggc
     5401 gatcgtcgcg gcttcgttgg cgatctgcag cggcgtcgcc cagacctggc tctgcccgat
     5461 gccggcgaaa caattcgtca tgcgattcat cgcgccgatc atcgccacgc ttcccggcaa
     5521 acgccccggc cgctcgggaa tgccgatgcc cgtgcgctgc ccgaatccga acgcgcgata
     5581 ccaatgatcg atccccgcgg gcccaagccg gtcagccacc gtttcgaaaa agacgttgca
     5641 actgcgctcg atcgcatcgg aatacgaaag ccaaccgtcg ggattcccgt cgtgcccccg
     5701 atgcggatac tccggcggaa tcgggtgatg ggcaacgctc atatgcagct gcgcgaggcg
     5761 atcggcgtat tcgctggcga cccagcaccg gccgatcgac tgccgcacct ttcgaccgtt
     5821 gtaatcgagg atcagatagc cggtgcactc gatgccctcg gtcggcccga gcacgccttg
     5881 cgtgatcgcg cccaggccca gcatcggctt gacggtcgat cccggctcga actcgtccat
     5941 cgtcgcgcgg ttcttcagcg ggttgttcaa gcgatcggcc atcaacgcgg cgtagcgcgt
     6001 atccagatcg ttggcgttga aatccggatt cgatgccatc acgcgcagct cgcccgtggc
     6061 gacatcgatc accaccgcgg ccgcgtgcat gttcagctcg acttccttcc gtcgcgccgg
     6121 gtcctcgtga aacaatgtca tcttcacgcg cttgagcatc tgttggactt cgcgctgaag
     6181 atcggcgtca atgctgaggc gaacatcctg ccccggcacg aaatcttcct gcttcacgat
     6241 cagcccgtcg ccgacgcgac gctcgatccg gccacgcgcg ccgcgcagca gtggctcgca
     6301 cagcgactcg atgccatctc gcccgatctg atcgttcggc agatacgcgc gcgtctcgtc
     6361 ccagccgtcc gctttggcgt cggcgacatc gcttgccgac acgcgcgcca ttcgccccag
     6421 cgtgtgacac gcgacttcct ccagcgggta ccgccggtgc gtgctggcct gcagtgacag
     6481 cccggggaac tgctccatcc gcttgcccag gaaattctgc gtgtccaaat caatattgcg
     6541 cagcacgacg tgcggctgct tctcgtcttc caccgtcacg gcaaagctgt cgatatccgg
     6601 cccctcaccg ccgccggtca accaccgctg ccaccgcggg gcatcggacg ccttggtgcg
     6661 attcagctga aagttgcgat accagaccca gcgcttgcgc atctcgacgc gacgctgaat
     6721 ctcgtggcgg atctcatcca ccgccgcccg gcgatcgaac gtgccgtcgg ccagcaccgg
     6781 atcgttgtcg tccggctgat acagctcggc cagcgtggcc cacatcgtct cgacatcggc
     6841 ctgcaccttg cggacttcca ggtctttgag cgtcgcccgc tgctccgccg gcatcttgcg
     6901 caggtccgcg ccaaagcgat tgcgcatgcg cgtgttggcg atcgcattca cccaagccgg
     6961 atcgggcggc tcggtgatcg cgcggtagtc aacgcaggca tcggtggagg cgacttcgat
     7021 cgccagctct ttgccggtga tgtcgaggat ccgcccgcgc gtcgtcgcgg tggtttgaga
     7081 tgtttgcagc agccccgcgg cacgattggt ccagtactga tgcgcgaaca cctgcacgtg
     7141 gatcgcgcgc gtgagcagga tgctggtgat cagcagcagg atgagcagga agatttttaa
     7201 gcgccgctcg aacatcggaa actatgcagc aacctgtttc gaagagacaa ggagaaaagg
     7261 agacaggggg acaggtggac taagaggatg aacgcagttc cgtttctatt ctccttgtcg
     7321 ccgtgccccc ttgtctcttc cgctctctgc cccgggtctt cgccagtacc tcagacaccg
     7381 gcgtcggggt ccttatcttt ctccgccgcg cgatcggcgc cgtcctgacg gttgaaaccc
     7441 gaacatccgc tgcatgcgtt gcaggatgcc gatgatgatc ggcgccagga tggcggtgta
     7501 aacgacgcac accagcagcg cccgcggcga ttgccttacc gcgggaatga cgtgaatgcc
     7561 atcggtgcgc gcgggtccga gcgggtgcac ccagccgtgg atcatcacga tggtcgacgt
     7621 gatcgtgccg ccgatcagcg tcatgaacag ttgcgtcaac ggatgctcgc gatacgccag
     7681 ctggcccacc gtcgtcacca gcatcgagac gatgccgtag gaaaacgcgt agaggcccat
     7741 cggctgcagc gtaaccaggt cctgcatcgc gcccagcagg aagctgccga gcagcgcctc
     7801 gtcgcgcggg gcgttgagcg agatgaagac gaccacgagc agcaccaggt tcggcgagat
     7861 gccgcggtat tgcacgtgag cgcccaggcc cagctgcagg ccgagcatca cgtacgcgag
     7921 gatgaagtag gcgaaccaac gcattgtctt ctaattgcct tgtcctgagc gcagcacgta
     7981 ctcg
//
LOCUS       DATIMH010000079         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2905988, whole genome shotgun sequence.
ACCESSION   DATIMH010000079 DATIMH010000000
VERSION     DATIMH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2905988"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..632
                     /locus_tag="VLI90_01370"
     CDS             39..632
                     /locus_tag="VLI90_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HSV12879.1"
                     /translation="MTRATTSTQDHATLPSPTHTGARTATAILAVCGCAVAFYLALYQ
                     WHVIAGVWDPFFRDPSGQYANGSERVLNSWLSRKLPVSDAFIGSLAYAIELIADLIGG
                     RERWRTAPWAVLVTAAVALAMGIGSAALVVIQPTLIHSWCTLCLTSAAISFIVLALVA
                     PEAWASIQYLRHRKSPQRPVAPPSHESQNYGADSART"
     gene            663..2351
                     /locus_tag="VLI90_01375"
     CDS             663..2351
                     /locus_tag="VLI90_01375"
                     /inference="COORDINATES: protein motif:HMM:NF006129.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HSV12880.1"
                     /translation="MADTVSDFIVQRLKEWGINRVYGYPGDGINGIMAALRQAHDQPR
                     FIQARHEELAAFMACAHAKFTGEVGVCMATSGPGAIHLLNGLYDAKLDHQPVVAIIGQ
                     TATTALGSNYQQEVDLQNLFKDVANEYVQTIASPAAARHIIDRAVRIAIDQRNVTCVI
                     VPKDIQEEKAVPEPPQKHDYALSGIGYPVPHIIPKRPELEAAARILNDGAKVAMLIGA
                     GALCAIDEVMQIAELLGAGVSKSWLGKAALADDVPYCCGSIGLLGTKPSYDLMQGCDT
                     LLVVGSSFPYGEFYPKSGQAKAVQIDLDGRMLSLRYPMDVNLKGDAKETLQALIPLID
                     RKRDRSWQNKIVEWVKDWWRVVEARAMNEANPINPERVFWELSPRLPDNCILSADSGT
                     AANWYARDIKIRRGMMASGSGNLATMGPAVPYAVAAKFCFPDRVAIALTGDGAMQMNG
                     LNACITVAKYWKEWSDPRWITLVLNNRDLNQVTWEQRIMTGDIKFNASQDLPDFPYAH
                     FAESIGLKGIRVEHPDQLGDAWDRALNSDRPCVLEAITDPDVPTLPPHITLQQA"
BASE COUNT          474 a          781 c          748 g          404 t
ORIGIN      
        1 cgtgctgcgc tcaggataaa cttacaagag ctccgcaaat gacgcgcgca acgacgagca
       61 cgcaggacca cgcgacgctg cctagcccca cgcacaccgg cgcgcggacc gccaccgcca
      121 tcctcgccgt ctgcggatgc gccgttgcct tctacctggc gctgtaccaa tggcatgtca
      181 tcgccggcgt gtgggatccg ttcttccgcg accccagcgg ccagtacgcc aacggcagcg
      241 agcgggtgct gaacagctgg ctgtcgcgca agctgccggt gtcagatgcg ttcatcggct
      301 cgctcgcgta cgcgatcgaa ctgatcgctg atctgatcgg cggccgtgaa cgctggcgca
      361 ccgcgccgtg ggcggtgctt gtcaccgcag cggtcgcgct cgccatgggc atcggcagcg
      421 ccgccttggt tgtcatccaa cccacgctga ttcacagctg gtgcaccttg tgcctgacgt
      481 ccgcggccat ctcattcatc gtactcgcgc tcgtcgcgcc tgaagcgtgg gcgagcattc
      541 aatacttgcg ccatcgcaaa tccccacagc ggcccgtcgc gccgccctca cacgaatctc
      601 aaaattacgg ggctgacagc gcacgcacat gacgatcaga atgcagtcga aggagtttca
      661 tcatggctga tacggtttcc gatttcattg ttcaacggct caaggaatgg ggcatcaacc
      721 gcgtctacgg atatcccggt gacgggatca acggcatcat ggctgccctg cgccaggcgc
      781 acgatcagcc gcgattcatc caggcgcggc acgaagaact ggcggcgttc atggcatgcg
      841 cgcatgcgaa gttcaccggt gaagtcgggg tatgcatggc caccagcggg cccggcgcga
      901 tccacctgct gaacgggctg tacgatgcca agctcgatca ccagccggtc gtggcgatca
      961 tcggtcagac cgccaccacc gcgctgggca gtaattatca acaggaagtt gatctgcaga
     1021 atctgttcaa ggacgtcgcc aacgaatacg tgcagacgat cgccagcccc gcggctgcgc
     1081 ggcacatcat cgaccgcgcc gtgcgcatcg cgatcgatca gcgcaacgtc acctgcgtca
     1141 tcgttccgaa ggacatccag gaagagaagg cggttcccga gccgccgcag aagcacgact
     1201 acgcgctcag cggcatcggc tatccggtac cgcacatcat ccccaagcgg ccggagcttg
     1261 aggcggcggc gcggatcctg aatgacggag cgaaggtcgc gatgttgatc ggcgccggcg
     1321 cgctgtgcgc aatcgatgag gtgatgcaga tcgccgaact gctcggcgcc ggtgtctcca
     1381 agagctggct cggcaaagcg gcgctggccg atgacgtgcc ctactgctgc ggctcgatcg
     1441 gcctgctggg caccaagccg agctacgacc tgatgcaggg ctgcgacacg ctgctggtcg
     1501 tcggctccag cttcccgtac ggcgagttct atccgaagtc cggtcaggcg aaggcggtgc
     1561 agatcgatct tgatggccgc atgctctcgc tgcgctatcc gatggacgtg aacctcaaag
     1621 gcgacgcgaa ggaaacgctg caggcgctga tcccgctgat cgaccgcaag cgcgatcggt
     1681 cctggcagaa caagatcgtc gaatgggtca aggattggtg gcgggtcgtc gaagcgcggg
     1741 cgatgaacga ggcgaacccg atcaaccccg agcgcgtctt ctgggaactg tcaccccggc
     1801 tgccggacaa ttgcattcta tccgccgaca gcggtaccgc cgccaattgg tacgcgcgcg
     1861 acatcaagat tcgccgcggc atgatggcca gcggcagcgg gaacctggcc accatgggcc
     1921 cggccgtgcc ttacgccgtc gccgccaagt tctgcttccc cgatcgtgtc gccatcgctc
     1981 tcaccggcga cggcgcgatg cagatgaacg gcctgaacgc gtgcatcacc gtcgccaaat
     2041 attggaagga atggagcgac ccgcgctgga tcacgctggt gctgaacaac cgcgacctga
     2101 accaggtgac gtgggaacaa cgcattatga ccggggacat caagttcaat gctagccagg
     2161 atctgccgga tttcccgtac gcgcacttcg ccgaatcgat cggcctgaaa ggcatccgcg
     2221 tggaacaccc ggatcaactg ggtgacgcgt gggatcgtgc gctgaacagc gatcgcccgt
     2281 gcgtgctgga agcgatcacc gatccggacg tgcccacgct gccgccgcac atcacgctgc
     2341 agcaggcgta gaatttcacg gagacgctgc tgaaggacga cccgaacgaa gcgggcatcc
     2401 tgaagct
//
LOCUS       DATIMH010000080         4674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_884017, whole genome shotgun sequence.
ACCESSION   DATIMH010000080 DATIMH010000000
VERSION     DATIMH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884017"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..977
                     /locus_tag="VLI90_01380"
     CDS             195..977
                     /locus_tag="VLI90_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12881.1"
                     /translation="MPRKRKHKSSHDFDSERPWTEAQWEAFMKESDSRAARYGELLET
                     FHEEPDGHEKVMREMGWLNEEDDEDEDPELAAEREQWIKEMNEAGEEALRELEAERAA
                     DEAAGITPDERRQIEEDEEKRAFPAYGAALDLGHRVIELFKPEFNRNPRKDPDAPRSR
                     AEEDEDELFHEAFMGIQMAGAKLTKAMAFEDDDDTRCANIVCCRWGMEAAEAGKAAWQ
                     RLRDYEKSPFTPAVVDDIVAAHAQVIEMIAAHIAEMRSRVWW"
     gene            1202..1486
                     /locus_tag="VLI90_01385"
     CDS             1202..1486
                     /locus_tag="VLI90_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HSV12882.1"
                     /translation="MTATTRPKLRARLKRVAGQIAGLQKMVDDDRYCVDILTQLAAAR
                     SALDAIGVELLTDHMEHCVAGCATGHPEARKRSHEQLVKEMRLALSRFLR"
     gene            1575..3971
                     /locus_tag="VLI90_01390"
     CDS             1575..3971
                     /locus_tag="VLI90_01390"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSV12883.1"
                     /translation="MHATDPSSATHDAAIAVNGMDCASCVAHVSKAARSVPGVRVCDV
                     NLARGRASVRFDPAQTTPDKIAAAITESGYPAAPESPGIAAGNVEEERLQRQMHHANQ
                     WLRRAIAGFVLWFPVEAAHWTLQIFFPHNHGAHGVLTWIAFLTSTIAITYVGSQFYKS
                     AWSALRRRTSNMDTLIALGASVAYVYSVVYFVGGLAHLWPAAMPERLYFMEASALLAL
                     VSLGHWLEARARQSAGSAIRELLNLAPATALRFRDVAATPASRLPILEAGDAGVAPTE
                     EVPVADLRVGDRVLVRPGDRVPIDGVVVDGNSSVDESMITGEPLPATRVPGDTVIGGT
                     VNQDGRLVVRAAKIGAETALAQIVQLVETAQASKPPVQKLADQIAAVFVPAVLLIALI
                     TAIGWYAWGAGHHWSPAATWAAIANAACSVLVIACPCALGLAVPAALMVGTGRGAKRG
                     ILIRDIDALQKAERIDTVVLDKTGTITRGRPSVVWFENKDALRAAAAAEQYSSHPLAQ
                     AIVRAARDAGLDIPPAASFQNDPGYGVVAEIEGRTVLVGSDDFLQKHEPGIAWGASQK
                     WDGAVGTEVKVAIKRSHGVEPFGTIRVADELKMDSVAAVNELHAMGLRTVLLTGDSAA
                     AGASIAKQVSIDDVRANVKPGGKADVIRELQRATRLADKLTTDAVGGRGNAGVAMVGD
                     GINDAPALAAADLGIALGSGSDVAKETGDIVLVGGSLHGVATAIRLSRATMRTIRQNL
                     ALAFLYNVLAIPLAAFGVLNPLIAAAAMALSDVTVIGNALLLRRAKIDAVDAADMKSS
                     "
     gene            4447..>4674
                     /locus_tag="VLI90_01395"
     CDS             4447..>4674
                     /locus_tag="VLI90_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12884.1"
                     /translation="MITLLAPKKSARLTDLPRTDEETMQQLLARLAGEAGDAPQERLT
                     PSAAKRRAKDEEEEDEKGDEDEAADDEEDVAD"
BASE COUNT          865 a         1408 c         1613 g          788 t
ORIGIN      
        1 ctcaaggggc aactgctcga cgtcgagatc gtcgacgcgc gaaacctgac gctgttcgga
       61 aagcttcaac ctgcgccagt ggcagcgctg tcataagcgc gcccggcgcg ccacaacaaa
      121 gtccacggct gtgccgtggg cccgactttc aaaagacgtc gcggggcata gcccgggctt
      181 ttggacattt caccatgccc cgcaaacgaa aacacaaatc ttcccacgac ttcgacagcg
      241 aacgcccctg gaccgaagcg caatgggaag ccttcatgaa agagagcgat tcgcgcgccg
      301 cccgctatgg cgagttgctc gagacgttcc acgaagagcc cgatggtcac gagaaggtca
      361 tgcgcgaaat gggctggctg aacgaggagg atgacgagga cgaggacccg gagctggccg
      421 ccgagcgcga gcagtggatc aaggagatga acgaggccgg cgaagaggcg ctgcgcgagc
      481 tggaggccga gcgcgccgct gacgaggccg cggggatcac gccggacgag cgccggcaga
      541 tcgaagagga cgaggagaag cgtgcgttcc cggcatatgg cgcggcgctg gatctgggtc
      601 atcgcgtgat cgagttgttc aagccagagt tcaatcgcaa tccccgcaag gatcccgacg
      661 ccccgcgcag ccgggccgag gaagacgaag acgaactgtt ccacgaggcg ttcatgggca
      721 tccagatggc gggcgcgaag ctgaccaagg caatggcgtt tgaggatgac gatgacacgc
      781 gctgcgccaa catcgtctgc tgccgctggg gcatggaagc cgccgaagcc ggcaaagccg
      841 cctggcagcg gctgcgggat tacgagaagt cgccgttcac gcccgcggtg gtggacgaca
      901 tcgtggccgc ccacgcccag gtgatcgaga tgatcgcggc ccacatcgcg gagatgcgct
      961 cgcgcgtgtg gtggtgagcg cgcggtgcac tccacgccgg gttcggggtg tacttgcgac
     1021 gacccggatg gatctgtcag tggtcagtcg tccgtggtcg gttgctgggg cgcgtgtacc
     1081 actaacaact gaccactgac aactgacaag taagtatccg gatcgtcgca agtacctccg
     1141 aacccggcgt ggtcggtata tcgaacttgc ctgatacccc ctgggggtat attatcactg
     1201 catgactgcg accactcgtc ctaagttacg cgcccggctc aagcgcgtcg ccggccaaat
     1261 cgccgggctg cagaagatgg tggatgacga ccgctattgc gtcgacatcc tcacccaatt
     1321 ggcggcggcg cgatccgcgc tggacgcgat cggcgtcgag ctgctcaccg accacatgga
     1381 acactgcgtc gccgggtgtg cgacggggca ccctgaggcg cgcaagcgtt cgcatgagca
     1441 gctggtcaag gaaatgcgct tggcgctgtc gcggttttta agatgactgc gtgattacgt
     1501 aattacgtga ttacgtgacg ccgcatcgac tcacgtaatc acgtaatcac ccgtcacctg
     1561 taacggcagt ctacatgcac gccaccgacc catcctccgc cacgcatgac gcggccatcg
     1621 ccgtcaatgg gatggactgc gcatcctgcg tcgcccacgt cagcaaggcc gcccggtcgg
     1681 tgccgggcgt gcgggtgtgc gatgtgaacc tcgcccgcgg acgcgcgagc gtgcgctttg
     1741 atccggccca gacgacgccc gataagatcg cggcggcgat cactgaatcg ggctatccgg
     1801 ccgcgccgga gtcgccgggc atcgccgcgg ggaacgtgga agaggaacgg ctgcagcggc
     1861 agatgcatca tgcgaaccaa tggctccgcc gggcgattgc cgggttcgtg ctgtggttcc
     1921 cggtggaagc ggcgcactgg acgctgcaga ttttctttcc gcataaccat ggcgcgcacg
     1981 gcgtgctcac gtggatcgct tttctcacca gcacgatcgc catcacgtat gtcggcagcc
     2041 agttttacaa gagcgcgtgg agcgcgctgc ggcggcggac gagcaacatg gacacgctga
     2101 tcgcgctagg cgcgagcgtg gcgtacgtct acagcgtcgt ctatttcgtc ggggggctgg
     2161 cgcatctgtg gccggcggcg atgccggagc gcttgtactt catggaggcg tcggcactgc
     2221 tggcgctggt cagcctgggg cattggctcg aggcgcgcgc ccggcaatcg gcgggcagcg
     2281 ccatccgtga gctgctcaat ctcgcgcccg caacagcgct gcgtttccgc gatgtagctg
     2341 cgacgcctgc gtcacgtttg ccgattctgg aagcgggcga cgcaggcgtc gcccctacgg
     2401 aggaagtgcc cgtcgcggat ttacgcgttg gcgatcgcgt gctcgtgcgt ccgggcgatc
     2461 gggtgccgat cgatggcgtg gtcgtcgatg gcaattcatc cgtcgatgaa tcgatgatca
     2521 ccggcgaacc cctgccggcg acgcgggtgc cgggcgacac ggtcatcggc gggaccgtca
     2581 atcaagacgg gcgattggtc gtgcgcgccg caaagatcgg cgccgagacg gcgttggcgc
     2641 agatcgtgca gctggtggag accgcgcaag ccagcaagcc accggtgcag aagttggcgg
     2701 accagatcgc ggcggtgttc gtgccggcgg tgttgctcat cgcgctgatc accgcgatcg
     2761 gctggtacgc gtggggcgcg ggccatcatt ggtcccccgc cgccacgtgg gcggcgatcg
     2821 ccaacgccgc gtgcagcgtg ctggtcatcg cctgcccgtg cgcgctgggc ctggcggtgc
     2881 ccgctgcgct catggtcggc accggccgcg gggcaaaacg cgggattttg attcgcgata
     2941 tcgacgcgct gcagaaggcg gagcgcatcg acacggtggt gcttgataag acggggacca
     3001 tcacgcgcgg ccgcccttcc gtcgtttggt ttgaaaataa agatgcactg cgcgccgcgg
     3061 cggccgcgga acaatacagc agccatccgc tcgctcaggc gatcgttcgc gccgcgcgcg
     3121 acgcggggct ggacattccg ccggccgcgt cgtttcaaaa cgatcccgga tatggagtcg
     3181 tcgccgagat tgaaggccgc acggttcttg ttggcagcga tgattttctg cagaagcacg
     3241 agcccggcat tgcgtgggga gcctcgcaga agtgggacgg cgcggtcggc accgaggtga
     3301 aggtggcgat caagcgttcg cacggcgtcg agccattcgg cacgattcgg gtagccgatg
     3361 aactgaagat ggattcggtt gccgcggtga atgagctgca cgcgatggga ctgcgcactg
     3421 tcctattgac tggcgacagc gccgccgcgg gggcgagcat tgcgaagcag gtgtcgatcg
     3481 acgacgttcg cgccaacgtc aagccgggcg ggaaggcgga cgtcattcgc gagttgcagc
     3541 gcgcgacgcg cttggcggac aagctgacga cggacgccgt cggcggccgc gggaatgcag
     3601 gtgtcgcgat ggtcggcgat ggcatcaacg atgcccccgc cttggcggcg gcggacctgg
     3661 gcatcgcgct gggcagcggc tcggacgttg cgaaggaaac cggcgacatc gtgctcgtcg
     3721 gcggcagcct gcacggcgta gccaccgcca tccgcctgtc gcgcgcgacg atgcggacga
     3781 tccgccaaaa cctggcgctg gcgttcctgt acaacgtgct ggcgatcccg ctggcggcgt
     3841 tcggggtgct caacccgctc atcgcggcgg cggcgatggc gctgtcggat gtcaccgtca
     3901 tcggcaacgc cctgctgctg cggcgggcga agattgatgc ggttgatgct gcggatatga
     3961 agagcagctg aacacggagg ttcggagata aagtcgacac ggagacacgg agacacggag
     4021 acacggagac gcggaggaag aggggatcaa ttcgcgtgct ccgtgattgt cggctttctc
     4081 gcggaatcgc cggcgacgag gcgttgtgaa gagaagagtt ttaagcacag atttcacaga
     4141 gtacgcagat tcagaagggc cattcttcaa tctgtgtcat ctgtgaaatc tgtggtttaa
     4201 aaactcttcc ttcgttccgt agcggctccg cgtttctcga tctgcgtttc ttacgacttt
     4261 cccgcttgac tcccccctac ggcctcgctt caataccgcc actttcggcg gcggtcggcc
     4321 ggcagaaggg ccgggctaat aaagttgatc ccgcgacgct ggattggtcg ttgacagtaa
     4381 cgcgagcctt cggttttcat acgcgcgcgc tgtggcaacg gcagacccct ttctgagatg
     4441 acccctatga tcacgctact tgccccgaag aaatcggccc gcttgaccga tctgccccgc
     4501 acggacgaag agacgatgca gcagctgctg gctcgcctgg ccggcgaggc cggcgacgcg
     4561 ccgcaggagc ggctgacacc gagtgccgcc aagcgccgcg ccaaggacga agaggaagag
     4621 gacgagaagg gagacgagga cgaggcggcg gacgacgaag aggatgtggc cgac
//
LOCUS       DATIMH010000081         4559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5215961, whole genome shotgun sequence.
ACCESSION   DATIMH010000081 DATIMH010000000
VERSION     DATIMH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5215961"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1255
                     /locus_tag="VLI90_01400"
     CDS             77..1255
                     /locus_tag="VLI90_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12885.1"
                     /translation="MSRFATGMPTFTFTNQGPAYDAFGDTTTGWTGYLLSVQADPGDA
                     IQLLDLGTSSSSTNGFFGTLLQDWWPGKKIATPTPVGVSATDTNGSFDGTDTHLLVTS
                     RVDMTAPFENSDLVHPVGAPPNDASDQWGTGDYLHGEFAIASANQLNVQPLAYLVVHN
                     GTTGSFSLDVVERSGVSGHIQSSFGSPAALTVVPVSSGPPVGFGLQSTTATGTTFSPN
                     GPKANAINIVSDGHGRYSPGFATGVDVITSPGGNGGNGFSFIGYVEISADPGSVGATQ
                     SIALELTNHGFPFESSGAQIQSIIDRINANSLGITASLMPASLASQYPDYNALLTVPT
                     PSADEYFGWDFNYPTFFEVGLGDVAAGISVPEPASASALALFATAALARRNKRRRLPR
                     "
     gene            complement(1291..2775)
                     /gene="ahcY"
                     /locus_tag="VLI90_01405"
     CDS             complement(1291..2775)
                     /gene="ahcY"
                     /locus_tag="VLI90_01405"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSV12886.1"
                     /translation="MTATLTDIPKSTKTPKGLEFKVRDLSLADWGRKEMLLAEQEMPG
                     LMAVRAEYAKQQPLKGLKIVGSLHMTIQTAVLIETLSALGAQVRWCSCNIFSTQDHAA
                     AAIAVGPKGTVENPQGVPVFAWKGETLDEYWWCTERALDFGNGAGPDQIVDDGGDVTL
                     LIHKGAEFEAAGTVPDFNKDSDPEEWGVILDLLRKTLKESPTRWTKVGANVKGVSEET
                     TTGVHRLYEMQNAGKLLFPAINVNDSVTKSKFDNLYGCRHSLVDGLFRATDVMISGKV
                     AVVCGYGDVGKGCCQSLKGQGARVIVTEIDPICALQAAMEGYQVLTLEDALPYADIFV
                     TTTGNHAIITAAHMARMKDKAIVGNIGHFDNEIDMAGLKKVKGIKKTNIKPQYDMWTF
                     PDGHSVLILAEGRLLNLGCATGHPSFVMSASFTNQTIAQIELATNRSKYEKKVYVLPK
                     ILDEKVARLHLDKLGVRLTKLTPDQAKYIGVPVEGPYKSDHYRY"
     gene            complement(2818..3117)
                     /locus_tag="VLI90_01410"
     CDS             complement(2818..3117)
                     /locus_tag="VLI90_01410"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSV12887.1"
                     /translation="MTKPIILHEEASKEFLEAEDYYFQQHPSLANEFKIEITRVIANV
                     ADRPKSYVKYLSGTRRSMADRFPYFVVFKEYRKAILVVAVAHVRREPGYWRERLK"
     gene            complement(3114..3347)
                     /locus_tag="VLI90_01415"
     CDS             complement(3114..3347)
                     /locus_tag="VLI90_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HSV12888.1"
                     /translation="MTKSAQQILKQAMALDDEDRAELVDELLGTLEPRVDQEYVTAWE
                     KEISSRIADFESGKAKGIPWEDVLKQLDERRRK"
     gene            complement(3360..4316)
                     /locus_tag="VLI90_01420"
     CDS             complement(3360..4316)
                     /locus_tag="VLI90_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSV12889.1"
                     /translation="MVNTAAQTDQLLAWMASLADPTRLRLLRVLERHELGVVELCDVL
                     QLPQSTVSRHLKVLGDQRWVRSRPQGTTRLYRMLLDELDAGARKLWLVAREQTTLWPT
                     ARQDELRVVRVLQQREIDSQSFFAGAAAEWDRLRSELYGERFSTAAMLALLPGDYAVA
                     DLGCGSGNLTAELAGCVKHVIGVDNSAEMLKAAKRRTAGLKNVDLRKGDLSALPIETG
                     ACDAAIIVLALTYVPDPAPAISEMARILKPGGRGVIVDLLPHDRDDFRRQMGQTSLGL
                     EPHPIERMMSGAGFTNVTTRPLPAEPNVKGPALFLASGAKSA"
BASE COUNT          820 a         1416 c         1369 g          954 t
ORIGIN      
        1 cggaggggtt tatcacatgc gaagcgaatg tagacgacgt gctgctgtcg cgggagcgac
       61 cgcgttgctt tggtcgatga gtcgtttcgc gacgggcatg ccgacgttca cctttacgaa
      121 tcaggggccg gcgtacgatg cctttggcga tacgaccacc gggtggaccg gctatctttt
      181 gagcgtgcag gccgatccgg gtgacgcgat tcagcttctt gatctcggta ccagctcgag
      241 cagcaccaac ggttttttcg ggacactcct tcaagactgg tggccgggga agaagatcgc
      301 aacgcccact ccagttggcg tctccgctac ggataccaac ggaagctttg acggaaccga
      361 cacgcacctg ctggttacct cgcgtgtgga tatgacggct ccgtttgaga acagcgatct
      421 ggtgcatccg gtcggcgcgc cgcccaatga tgcatcggat caatggggca ccggcgatta
      481 cctgcacggc gaattcgcga tcgcttccgc gaaccagctg aacgttcagc ccttggcata
      541 cctcgtggtg cacaacggca ccaccggtag tttttcgttg gacgtcgtcg agcgctccgg
      601 cgtttcgggg cacattcaat caagcttcgg cagccccgcg gcgctgacgg tggtgcccgt
      661 ttcgtctggg ccgccagtgg gattcggttt gcaatcgacg accgccaccg ggaccacgtt
      721 ctcacccaac ggaccaaaag cgaacgcgat caacatcgtc tccgacggcc atggacgcta
      781 ttcaccgggt ttcgcgacgg gcgttgacgt catcacatca cctggcggta acggcgggaa
      841 tgggttctcg ttcatcggat acgttgagat cagcgccgac cccggcagcg ttggcgcgac
      901 ccagtcgatc gcgctagaac tgaccaacca cggcttcccg tttgaatcaa gcggggcaca
      961 aatccagtcg atcatcgacc ggatcaatgc caacagcttg ggcatcaccg ccagcttgat
     1021 gccggcgtct ttggcgtcgc agtatccgga ctacaacgcg ctgctgaccg tgccgacgcc
     1081 gagcgctgac gagtatttcg gatgggactt caactatccg acgtttttcg aagttggtct
     1141 cggcgacgtt gccgcgggca tctcagtccc cgaacccgct tcggcaagcg cgctggcgct
     1201 gttcgcgacc gctgcgctcg cgcggcgcaa taaacgaaga aggctcccgc gatagccgga
     1261 gcctccttcg ttgaattcac ttcgtcagca tcaataccgg taatggtccg acttatacgg
     1321 cccttccacc ggcacgccga tgtatttggc ctggtccggc gtgagcttgg tcagccgcac
     1381 gcccagcttg tccaggtgca ggcgagcgac cttttcgtcg agaatcttcg gcaggacgta
     1441 caccttcttc tcgtacttgc tgcggttggt ggccagctcg atctgggcga tcgtctggtt
     1501 ggtgaagctc gcgctcatca cgaagctcgg gtggcccgtg gcgcagccga ggttcagcag
     1561 ccggccttcg gccaggatca ggacgctgtg accgtcgggg aaggtccaca tgtcgtactg
     1621 cggcttgatg ttcgtcttct taatcccctt caccttctta agccccgcca tgtcgatctc
     1681 gttgtcgaag tggccgatgt tgccgacgat cgccttgtct ttcatccgcg ccatgtgggc
     1741 ggcggtgatg atcgcgtggt tgccggtggt ggtgacgaag atgtcggcgt acggcagggc
     1801 gtcttccagc gtcaggacct gatagccttc catggcagct tgaagcgcgc agatcggatc
     1861 gatttcggtg acgatcacgc gggcgccctg gcctttgagt gactgacagc aacccttgcc
     1921 gacgtcgccg tagccgcaga cgacggcgac tttgccgctg atcatgacgt cggtggcgcg
     1981 gaacagacca tcgacgagcg agtggcggca accgtagagg ttgtcgaact tgctcttggt
     2041 cacgctgtcg ttgacgttga tggcgggaaa gagcagcttg cccgcgttct gcatttcgta
     2101 aaggcggtgg acgccggtcg tggtttcttc agaaacgcct ttgacgttgg cgccaacctt
     2161 ggtccagcgc gtcggtgatt ccttcagcgt cttgcgcagc aggtcgagga tcacgcccca
     2221 ttcttcgggg tcgctgtcct tgttgaagtc cggcaccgtc ccggcggctt cgaattcggc
     2281 gcccttgtgg atcagcagcg tgacgtcgcc accgtcgtcc acgatctgat cgggaccggc
     2341 gccgttgccg aagtcgagcg cgcgttcggt gcaccaccag tattcgtcca acgtctcgcc
     2401 cttccaggcg aagacgggaa cgccctgcgg attctcgacc gtgccctttg ggcccacggc
     2461 aatcgcggcg gcggcgtggt cctgcgtgct gaagatgttg caggaacacc agcgcacctg
     2521 cgcgcccagg gccgacagcg tttcgatcag caccgccgtc tggatcgtca tgtgcagcga
     2581 gccgacgatc ttcaggccct tgagcggttg ctgcttcgcg tactccgccc gaacggccat
     2641 caggcccggc atctcctgct cggcgagcag catttccttg cggccccagt cggccagcga
     2701 caggtcgcgg actttgaact ccaggccctt gggggtcttg gtggatttgg ggatatcggt
     2761 cagcgtggcg gtcatcggtt ctcctgaatg taagtccgca atgcggatca gtcggtacta
     2821 cttcaatctt tctcgccaat agccaggctc gcgtctcaca tgcgcgaccg ctaccacgag
     2881 aatcgctttc ctgtactctt tgaagacgac gaagtacgga aagcgatcag ccattgagcg
     2941 gcgcgtgccc gatagatatt tgacgtacga cttcgggcga tcagcgacat tggcaattac
     3001 ccgcgtgatt tcgatcttga actcattggc cagcgacggg tgctgctgaa aataataatc
     3061 ttctgcttcg agaaactctt tggaagcctc ttcatgaagg atgatcggct tggtcatttc
     3121 cgccgccgct catccaactg cttgagcaca tcttcccacg gtatgccctt ggcctttccg
     3181 ctttcgaaat cggcaatgcg cgaggagatc tccttctccc acgccgtcac gtattcctga
     3241 tcgactcgcg gctcgagtgt ccccaaaagc tcatcgacca gttcggcacg atcttcgtca
     3301 tcgagcgcca tcgcctgctt gaggatttgc tgagccgatt tcgtcatgcc accattctat
     3361 caggctgatt tcgcgccgct cgccaaaaac agcgccggcc ccttcacgtt cggctcggcc
     3421 ggcagcgggc gcgtcgtcac gttggtaaac ccagcgccgc tcatcatccg ctcgatcgga
     3481 tgcggttcaa gccccaggct cgtctggccc atctgccggc ggaagtcgtc gcgatcgtgc
     3541 gggagcagat cgacgatcac accgcggccg ccgggtttca ggatcctcgc catctcgctg
     3601 atggcgggcg ccggatcggg cacatatgtc agcgcgagca cgatgatcgc cgcatcgcag
     3661 gcgccggttt cgatcggcaa tgcggacaga tcgcctttac gcagatcgac gttcttcaac
     3721 cccgcggtgc ggcgtttcgc cgccttcaac atctcggcgg agttgtctac gccgatcaca
     3781 tgtttgacgc atccagccag ttccgccgtc agattgccgc tgccgcagcc taaatcagcg
     3841 acggcgtaat cgcccggcag cagcgcgagc atcgccgcgg tggagaagcg ttcgccgtaa
     3901 agttcactgc gaagccgatc ccactcggcg gcggcgccgg cgaagaagga ctggctgtcg
     3961 atctcgcgct gctgcagcac gcgaacgacg cgcagctcgt cctgtcgcgc ggtcggccaa
     4021 agagtggtct gctcgcgcgc gacgagccac agttttcgcg cgcccgcgtc cagctcgtcc
     4081 agcagcatgc ggtacaagcg cgtcgtgcct tgcggccggc tgcggaccca gcgctggtcg
     4141 cccaacacct tcaggtgccg gctgaccgtg gactgcggca actgcagcac atcgcacagt
     4201 tcgaccacgc ccagctcgtg gcgttccagc acgcgcagca agcgaaggcg ggttgggtcg
     4261 gccagcgacg ccatccacgc cagcaactgg tctgtttgag ccgcggtatt aaccatccgt
     4321 tgatccggat ggaatgttac tgcgcgagtc ccagacggtc aaggagcagc caagaaggca
     4381 tatttccgag gggcgcaaaa ccgtaattct attgcgcaag aacgcaattg tgttttaacc
     4441 cgtttcgcaa taccttgtgc tagtctgagc agtggagagc gagagagagc aagtctggcc
     4501 gatagtttca cgggccatca caaaacacct cctgacttgg acatgggcga gaaacacac
//
LOCUS       DATIMH010000082         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4639482, whole genome shotgun sequence.
ACCESSION   DATIMH010000082 DATIMH010000000
VERSION     DATIMH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4639482"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VLI90_01425"
     CDS             <1..281
                     /locus_tag="VLI90_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12890.1"
                     /translation="AASIRRRASRSEAVRERRDALAQGALVRAFGSELFNAAWNLGDD
                     SPANVAATPASPLRQSARQASRLHRFFHAFGGIRSIFARLLLHYDERP"
     gene            268..1563
                     /locus_tag="VLI90_01430"
     CDS             268..1563
                     /locus_tag="VLI90_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSV12891.1"
                     /translation="MNGRDYALIQLDGRRLPRWPDQLLRGQKQLVAPPDPRDRALGEQ
                     ITAGVVKNLLLLQYLIEHYSGRPLKNIDSLVQKILAVALYQLRFLTRVPANAAVDEAV
                     KQAKRFGRTKAAGFVNAVLRNATREPDPPLPAEQDDPQGFGEIVLSHPPQLLARLTEL
                     LGIADALRVCRHDNAEPPTIVRLFAGQSPEALHAQGVTFTPHEQPGMFVVEGAKHAVL
                     AAWAKAGVAQVQDPTAAIVADACDVSPGQIVLDRCAGLGTKTLQLRERLGDTGELFAV
                     DASAARCEGLQQLIAARGLHNVHVSHGSMLSKLGDQLPRTFDRVLVDAPCSNSGVLAR
                     RPEARYAQDENTLASLAKLQRSILKDTAPHVASDGLLVYSTCSIWPEENERMVAVFLR
                     EHPSFELVDERATLPSLSDDAARYRDGGYFAVMRKRVYP"
     gene            1697..1870
                     /locus_tag="VLI90_01435"
     CDS             1697..1870
                     /locus_tag="VLI90_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12892.1"
                     /translation="MSWILIAFTVLAAWAVLRIFGGERQRQVQLLQAQILQQGSEAAR
                     AAGSAPAQNHGAR"
BASE COUNT          352 a          668 c          708 g          380 t
ORIGIN      
        1 gagcggcatc gatccgaaga cgggcgagcc gcagtgaagc agtgcgcgag cggcgcgacg
       61 ccctggcgca aggcgcgctc gtccgcgcct ttggttccga gctattcaac gccgcttgga
      121 atctgggcga cgattcacct gcgaatgtag ccgcgacgcc tgcgtcgcct ttgcgccaat
      181 cggcgaggca ggcgtcgcgg ctacatcggt tcttccacgc cttcggcggt atccggtcca
      241 tcttcgcgag gctactgttg cattacgatg aacggccgtg actatgcgct gatccaactc
      301 gacgggcggc gattgccgcg ctggccggac caactgctgc gcgggcagaa gcaacttgta
      361 gcgccccccg acccgcgcga tcgggcgctt ggcgagcaga tcaccgccgg cgtcgtcaag
      421 aacctcctgc tgctgcagta tctcatcgag cactactccg gccggccgct gaagaacatc
      481 gactcgctgg tgcagaagat cctggcggtg gcgctgtatc agctgcggtt cctcacgcgc
      541 gtgccggcga acgccgcggt ggacgaggcg gtgaaacagg cgaagcgttt cggccgaacg
      601 aaagccgcgg ggttcgtgaa cgcggtgttg cggaacgcga cgcgcgagcc ggatccaccg
      661 ctgccggcgg agcaggatga tccccaaggc ttcggcgaga tcgtgctttc acatccgcca
      721 cagctgctgg cgcgattgac ggaactgctc ggcattgccg atgcgctgcg ggtctgccgg
      781 cacgacaacg ccgagccgcc gacgatcgtc cgcctcttcg cggggcaatc gccggaggcg
      841 ctgcatgccc agggcgtgac cttcacgccg cacgagcagc ccggcatgtt cgtcgtcgaa
      901 ggcgcaaaac acgcggtcct ggcggcgtgg gcgaaagcag gcgtcgcgca ggtgcaggat
      961 ccgacggcgg cgatcgtcgc cgacgcgtgc gatgtatcgc cggggcagat cgtactggat
     1021 cgatgcgctg gactagggac gaagacgctt caactgcgcg agcgcttggg cgatacaggc
     1081 gagctcttcg cggtcgatgc atcggccgcg cgatgtgaag gtcttcagca gttgatcgcg
     1141 gctcgtggac tccacaacgt ccatgtctcc cacggctcga tgctatctaa attgggcgac
     1201 caacttccgc gcacgttcga ccgcgtcctg gttgacgccc cgtgcagcaa cagcggcgtg
     1261 ttggcgcgcc ggccggaagc gcgatatgcg caggatgaga acacgctggc gtcgctggcg
     1321 aagctgcagc ggagcatctt gaaggacacg gcgccgcatg tcgcttcgga tgggttgctc
     1381 gtctacagca cctgcagcat ttggccggag gaaaatgaaa ggatggtggc agtgtttctg
     1441 cgcgagcacc cgtcgttcga gctggtggac gagcgggcga cgttgccgag cttgagcgat
     1501 gatgccgcgc gctatcgcga tggtggatat ttcgcggtga tgcgcaagcg cgtctacccg
     1561 taaccacgcc ggcttcggtg ggtcctcgcg acgaagcgag attctgcggc gcgccagaac
     1621 cattcgggtc gtcggcgtac ctccgatccc ggcgtgccaa ttcacctccg ctgtccttcc
     1681 tgccgatgat gttgacgtga gctggatcct catcgccttc accgtcctgg ccgcgtgggc
     1741 ggtgctgcgc atcttcggcg gcgaacgcca gcggcaggtt cagctgttgc aagcgcagat
     1801 cctgcagcag gggtctgagg cggctcgcgc tgccggttcg gcgccggcgc aaaatcacgg
     1861 cgctcgttga cagcaaatcc ggaccagcga tacacttctg ttgtgacggt ctcagccaat
     1921 tcggcatgcg ctcgcccaaa ttgattcaca tccggaggtg accgtggaga agcccaagac
     1981 accgccccat ccgctgccgt cgctcaactg ggtttactgg ttggccctgc tgttttcgtt
     2041 ggcggtgctg gggctgggcg catattacgc cgcccacgcg accggcagct tcacgctgct
     2101 ggccaccg
//
LOCUS       DATIMH010000083         3454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2328154, whole genome shotgun sequence.
ACCESSION   DATIMH010000083 DATIMH010000000
VERSION     DATIMH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328154"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="VLI90_01440"
     CDS             <1..2010
                     /locus_tag="VLI90_01440"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HSV12893.1"
                     /translation="AYWQQVVEPLLADAGTMCGKTLTRLQTDSWEMGLCNWTDDFAEQ
                     FRSRRGYEITPYIAALAGKIVGSRDVTNRFLYDFRKTVGDCIADDHYRAFAERAHARG
                     LGIQCESGGPHGAPIDALKCLGRDDMPMGEFWAPSNQHRVKPQERFFMKQSASSAHIY
                     GHPRACGEGFTSIGPHWEETPWSLKTAFDTEACEGLNLIYWHAFVCSPAETGMPGQQY
                     FAGSHLNPKVTWWNDAGAFMSYMNRCQYLLQQGLFVADAVYYYGDSVPNFARLKSSDP
                     AHVLPGRDYDVCDEEVLLTRMSVKGARIVLPDGMTYRVLVLPDRQSMPPAVLAKIAEL
                     VKAGATVIGPKPTAAAGLADYPQCDAKVAALADEVWGTCDGKTITENRYGQGRVVWGK
                     TAKQVLEADGVPMDFATEAKLDYIHRAGADGDIYFITNPADVPVDTTCTFRVSAKAAQ
                     MWWPDYGKIHRAAMTSGNGTAAMPLHLNGRGSMFVVFGAAADGPPQSLAVDSTSAPPI
                     EINGLWTVHFEPGWGAPESIAFPKLISWTDNADPNIKFFSGTATYSVDVGVPAGDHDW
                     VLDLGEVDDLARVKVNGTDVGVVWEKPARIDVSGAWRPGRNTIEIAVTNLWPNRLIGD
                     AGLPPEQRRTRTNIRKFTGKSPLRPSGLLGPVRLIPQAAQQETTQ"
     gene            2007..>3454
                     /locus_tag="VLI90_01445"
     CDS             2007..>3454
                     /locus_tag="VLI90_01445"
                     /inference="COORDINATES: protein motif:HMM:NF027224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HSV12894.1"
                     /translation="MIRAKRNKQLGQRIAGAIFATAITASAATAAGPYGTVSFSSVGT
                     GAYGVGDINSCAVTLNTLTTVGNHQFIAYYDSSGAIMIGRRDTGSSAWTTFNSGITLA
                     SNLLSDDHNVIAIAVDGAGFMHVGWGMHNVALDYAISNATVTGATLSSVSFTQQTSAN
                     APTLFPNAGSTTNEATYPQFYNIPGSNNLLFTYRNGGAGGGSGNGNQYFDVYNPATHT
                     WTNNFVINGELTSVNAYVNRMVYTSTNNLLMTWTWRATSNWQTNSNIMFAQSPDNGAS
                     WYQQGGTTKYDLPIIQHTVSGPGGNTDTKSVAQVIANIPQGDSFINQTSMTVDNNDHP
                     IVATYLAPGWTPTSATAGSGNPNRQYMLYFYTGAQWMSSQVSHRTSDTAVDFSGADVR
                     DLGRPDVLVDKQGRVLVVTRSEDSAMGSYSNPATPNNGIVVYYTTTTALYAGTPNWRS
                     VTLDAANMGSYEPTYDPALWSQTNQFNLFYEPV"
BASE COUNT          665 a         1065 c         1151 g          573 t
ORIGIN      
        1 gcgtattggc agcaggtcgt cgagccgctg ctggccgacg cgggaacaat gtgtggcaag
       61 acgttgaccc ggctgcagac cgacagctgg gagatgggtc tgtgcaactg gacggacgat
      121 ttcgcggagc aattccgcag ccgccgcggt tatgagatca cgccgtacat cgcggcactg
      181 gcggggaaga tcgtcggcag ccgggatgtc accaatcggt ttctttatga ctttcgcaag
      241 acggttggcg actgcatcgc cgacgatcac tatcgcgcct ttgccgagcg ggcgcacgcg
      301 cgcgggctgg gcatccagtg tgaatctggc ggaccgcacg gggcgccgat cgatgcgctg
      361 aaatgcctcg gtcgcgacga catgccgatg ggggaattct gggcgccgtc gaaccagcac
      421 cgcgtgaagc cgcaggagcg gttcttcatg aagcagagcg cgtcgtccgc gcatatttac
      481 ggtcatccgc gcgcctgtgg cgaggggttc acgtcgatcg gcccgcactg ggaagagacg
      541 ccgtggagct tgaagacggc gttcgacacc gaggcgtgcg agggactgaa cctcatctac
      601 tggcacgcgt tcgtctgttc gccggcggaa accggcatgc cggggcagca gtatttcgcg
      661 gggtcgcacc tgaacccgaa ggtgacgtgg tggaacgacg cgggcgcgtt catgtcgtac
      721 atgaaccgct gccagtacct gctgcagcag gggctgtttg tcgcagatgc ggtgtattac
      781 tacggcgaca gtgtgccgaa cttcgcgcgg ctcaagagca gcgatcccgc gcacgtgctg
      841 ccggggcgcg attacgatgt atgcgatgaa gaggtgctgc tgacgcggat gagtgtgaag
      901 ggcgcgcgga tcgtgctgcc ggatggcatg acctatcgcg tgctggtgct gccggaccgg
      961 cagtcgatgc cgccggcggt gctggcgaag atcgccgagc tggtgaaggc gggggcgacg
     1021 gtgatcggtc ccaagccgac cgccgcggcg gggctggcgg attatcccca gtgcgacgcg
     1081 aaggtggcgg cgctggcgga tgaggtgtgg ggcacgtgcg acgggaagac gatcacggaa
     1141 aatcgatacg gccagggccg ggtcgtgtgg ggcaagacgg cgaagcaggt gctggaagcg
     1201 gacggcgtgc cgatggattt cgcgacggag gcaaagctgg attacatcca tcgcgccggc
     1261 gcggacggag acatttattt catcaccaac cccgcggatg tgccggttga tacgacctgc
     1321 acgttccgcg tgagcgccaa ggccgcgcag atgtggtggc cggattatgg aaagattcat
     1381 cgcgcggcaa tgacgagcgg caacggcacc gcggcaatgc cgctgcacct caacgggcgt
     1441 gggtcgatgt tcgttgtatt cggcgcagca gcggatgggc cacctcaatc gctcgctgtc
     1501 gattcaacca gcgcaccacc gatcgagatc aacggactgt ggaccgttca cttcgagccc
     1561 ggctggggcg cgccggagtc gatcgcgttt ccgaagctga tctcctggac cgacaacgcg
     1621 gatcccaaca tcaagttctt ctccggcacc gcgacgtaca gcgtcgatgt gggcgttccg
     1681 gctggcgatc acgattgggt gctggacctg ggcgaggtgg acgatctggc ccgcgtgaag
     1741 gtgaacggca ccgacgtcgg cgtcgtgtgg gaaaagccgg cgcggattga cgtcagcggc
     1801 gcgtggcgcc ccgggcgtaa cacgatcgaa atcgccgtta cgaacctctg gcccaatcgc
     1861 ctgatcggcg acgcgggcct gccgccggag cagcggcgga cgcgcacgaa catccgcaag
     1921 ttcactggca agtcaccgct gcggcccagc ggtctgctcg gtccggtgcg actgatcccg
     1981 caagcggcgc agcaggagac cactcagtga ttcgtgcgaa gcgaaacaag caactcggac
     2041 aacgcatcgc tggtgccatc ttcgccactg cgatcaccgc gagcgctgcc accgccgccg
     2101 ggccttacgg caccgtttcg ttctccagcg tcggcaccgg cgcgtacggc gttggcgaca
     2161 tcaactcctg cgccgtcacg ctcaacacgc tcacgaccgt cgggaaccat cagttcatcg
     2221 cctattacga cagcagcggc gcgatcatga tcggccggcg cgacacggga tcgtccgcgt
     2281 ggaccacctt caactccggc atcacgctgg cgtcgaacct gctgagcgat gaccacaacg
     2341 tgattgcgat tgccgttgat ggcgcgggct tcatgcacgt gggctggggg atgcacaacg
     2401 tcgcgctgga ttatgcgatc tccaacgcga cggtgacggg agcgacgctc agctccgtct
     2461 cgttcaccca gcagacgtcg gccaacgcgc cgacgctatt tcccaacgcc ggctcgacca
     2521 ccaacgaggc cacctacccg cagttctaca acatccccgg cagcaacaac ctcctgttca
     2581 cctatcgcaa cggcggcgct ggcgggggca gcgggaacgg caaccagtat ttcgacgtct
     2641 acaacccggc cacccatacg tggaccaaca acttcgtcat caacggggaa ctcaccagcg
     2701 tcaacgcgta cgtcaatcgc atggtctaca cgtccaccaa caacctgctg atgacgtgga
     2761 cctggcgggc gacgtcgaac tggcagacga actcgaacat catgttcgcg cagtcgcccg
     2821 acaatggggc gagctggtat cagcagggcg ggacgacgaa gtacgacctg ccgatcatcc
     2881 agcacaccgt ctccggtccc ggcgggaaca ccgacaccaa atccgtcgcg caggtgattg
     2941 ctaacatccc gcaaggcgac agcttcatca accagaccag catgacggtg gacaacaacg
     3001 accacccgat cgttgcaacg tatctggcgc ccggctggac gccgacgtcg gccaccgcgg
     3061 ggtcggggaa tccgaaccgg cagtacatgc tgtacttcta cacgggcgcg cagtggatgt
     3121 cgtcgcaggt gagccatcgc acgtcggaca ccgcggtcga tttcagcggc gcggacgtgc
     3181 gcgacctggg ccggccggac gtgctggtgg acaagcaggg ccgcgtgctg gtggtgacgc
     3241 ggtcggaaga ttcggcgatg ggcagctatt cgaatccggc cacgccgaac aacggcatcg
     3301 tcgtttatta cacgacgacg actgcgttgt atgcgggaac tcccaactgg cggtcggtca
     3361 cgcttgacgc agcgaacatg ggatcgtacg agccgacgta cgacccggcg ctctggtctc
     3421 agacgaatca gttcaacctg ttctacgagc cggt
//
LOCUS       DATIMH010000084         3685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1751581, whole genome shotgun sequence.
ACCESSION   DATIMH010000084 DATIMH010000000
VERSION     DATIMH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751581"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1371
                     /locus_tag="VLI90_01450"
     CDS             31..1371
                     /locus_tag="VLI90_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12895.1"
                     /translation="MNHGKRLARSYGGAFALAAATALFGPSIVRAQTPPPNNAADDQS
                     PPTRQEFNAVKEELRQTKAELADVKQKSEAAPSSADMDDFDRRLKEIGDAANRALPGR
                     ETIVIAGDASVGFSVPRHGHSSFDAGVAPLILYRPVDHLLFEAAADIGVSTDETGSSS
                     TSFDLTIADASYEVCDGLVVGAGLFVVPFGVYHNHFDPPWINKFPDDPLPFADGGIAP
                     GSEVGIFARGAVPVRSMKVTYDVYLTNGPQLITNDPAAAGSLNFDDFTDINNNKAVGG
                     RFGFLPVPNAEMGYSIQYSKPGADPYGDVTALLQAVDLNWRQEVTPLMGVFDIRGEWV
                     WTNIGTATYDPLGTAGFGPVTFGSHRNGGYAQLCYRPTLAGNKVIRNFELVSRFDFQE
                     IPLDVPGGEREQRWIFGVDYWFAPNVVAKVAYELDNKTVGDDQNALLVQLGIGL"
     gene            1409..1762
                     /locus_tag="VLI90_01455"
     CDS             1409..1762
                     /locus_tag="VLI90_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSV12896.1"
                     /translation="MRKQLKLKNEIVVAAGVVLAMTMFACKSNNDQPQSMSTAAAAAA
                     PTTQPVMANMFGPLPEKGGAQLWAENCNRCHYAPAPDRYSDAQWDVVVQHMRMRANLT
                     GEEARQIAVFLKSAN"
     gene            complement(1847..>3685)
                     /gene="acs"
                     /locus_tag="VLI90_01460"
     CDS             complement(1847..>3685)
                     /gene="acs"
                     /locus_tag="VLI90_01460"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HSV12897.1"
                     /translation="PDPFWGKIAGDFHWFKRWDQVLQWKAPHAKWFVGGQTNVAYNCL
                     DRQIEQGRGDKTAIIFEAEPISEGGNHDVRRLSYKQLKDEVCRLANGLKSLGIRRGDR
                     VTIYMPMLAEAAIAMLACARIGAAHSVIFGGFSSQAIADRVEDAQSNYLITADGGYRR
                     GAIVPLKKNVDDALTKTDKIKKVVVLRRTGGNINWVEGRDVWWNDVIANQSTDCPAEP
                     MDAEDLLFVLYTSGSTGKPKGIIHTTAGFMVGTSITTKYVFDLHDDDVYWCTADVGWI
                     TGHSYVVYGPLSNGATVLMYEGAPNFPDFSRFWSIIERHKVTLFYTAPTAIRAFMRAG
                     RELVDKHNLSSLRLLGTVGEPINPEAWMWYRDVIGHNRCPIVDTWWQTETGMIMITPL
                     PGATPTKPGTATLPFFGVDAAIVTRDGQELGPNQGGLLVVRKPWPAMLRGIFNDPDRY
                     VRQYWSDVKGMYFTGDGARRDHDGYFWIMGRVDDVLNVSGHRLGTAEIESALVAHPSV
                     AEAACVGMPHELKGQGIAAFVTLKSGQRPTDQLKKELIDGVARAIGSLAKPDQIRFTD
                     ALPKTRSGKIMRRLLKELASGGQVKGDTTTLEDFSVLAKLKESDEG"
BASE COUNT          660 a         1184 c         1148 g          693 t
ORIGIN      
        1 cgccagtcag cccggggaag gagccaacaa gtgaatcatg gcaaacgcct cgcgcgctcg
       61 tacggaggag cgttcgcgct cgcggccgcc accgcgttgt tcggtccgtc catcgtccgc
      121 gcgcagacgc ccccacccaa caatgcggca gacgatcagt cacctcccac gcggcaggag
      181 ttcaacgccg tgaaggagga gctgcgccag accaaggcgg aactggccga tgtcaagcag
      241 aagagcgagg ccgcgccgtc gtcggccgac atggacgatt tcgatcgccg gttgaaggag
      301 atcggcgatg ccgccaaccg ggcgctgccc ggcagggaga cgatcgtcat cgccggtgac
      361 gcgtcggtcg gtttcagcgt gccgcggcac ggtcactcgt cgttcgacgc cggcgtcgcg
      421 ccgctgatcc tgtatcgccc ggtcgatcac ctgctgttcg aagccgccgc ggatattggc
      481 gtcagcaccg acgaaaccgg ctcgtccagc accagcttcg atctcacgat cgccgatgct
      541 tcgtatgaag tgtgtgacgg cctggtcgtc ggggcgggcc tcttcgtcgt gccgttcggc
      601 gtgtatcaca atcacttcga tccgccgtgg atcaacaagt tccctgatga cccgctcccc
      661 ttcgcggacg gcggcatcgc accgggttct gaagtcggca tcttcgcccg cggcgcggtt
      721 ccggttcgct cgatgaaggt gacctatgat gtgtatttga ccaatggccc ccagttgatc
      781 accaacgatc ccgccgcggc gggcagcttg aacttcgacg acttcacgga catcaacaac
      841 aacaaagccg tcggtggacg cttcggcttt ctgccggtgc cgaacgccga gatgggttac
      901 tccatccaat actcaaagcc cggcgccgat ccgtatggag acgtgacggc actgctgcag
      961 gcggtcgatc tgaactggcg gcaggaggtg accccgctga tgggcgtgtt cgacatccgc
     1021 ggggaatggg tgtggaccaa catcggcacc gccacttacg atcccctcgg caccgctggt
     1081 ttcggtccgg tgacgttcgg ctcacatcgc aacggcggat acgcccagct gtgctaccga
     1141 ccgacgttgg ccggcaacaa ggtcattcgc aactttgaac tggtcagccg attcgatttc
     1201 caggaaattc cgctcgacgt ccccggcggt gagcgcgagc agcgatggat cttcggcgtt
     1261 gattactggt tcgcacccaa cgtcgtcgcc aaggtcgctt atgagttgga taacaagacc
     1321 gtcggagatg accagaacgc cctgctggtt caactcggca tcggcctgta ggtagaggcc
     1381 cggtaaaccg acaacgcgag gagtcgccat gcgaaagcaa ctcaaattga aaaacgaaat
     1441 cgtcgtcgcg gccggcgtcg tactggcgat gacgatgttt gcctgcaaga gcaacaacga
     1501 tcaaccgcag tcgatgtcca ccgccgcggc cgcggccgcg ccgaccaccc aaccggtgat
     1561 ggccaacatg ttcggccccc tgcccgaaaa gggcggcgcc cagctgtggg cggagaactg
     1621 caaccgctgc cactacgccc ctgcgccgga tcgctacagc gatgcgcagt gggacgtggt
     1681 cgtgcagcac atgcggatgc gcgcgaacct gaccggcgaa gaagcccgcc agatcgcggt
     1741 gttcctcaag tccgcgaatt gatcggtcct gaacgatcca agagcgaaac gacgaaccgg
     1801 cccgatccgc ttgagcgggt cggccagcgg cgccaggcgc agctggttat ccctcgtccg
     1861 actctttcaa cttcgccaac acgctgaaat cttccagcgt ggtcgtgtcc cccttcactt
     1921 gcccgccgct ggcgagttct ttgagcagcc ggcgcatgat ctttccgctg cgggttttcg
     1981 gcagcgcatc ggtgaaacga atctggtccg gcttcgcgag gctgccgatg gcgcgggcga
     2041 cgccgtcgat cagctctttc ttcagctggt ccgtcgggcg ctggccggat ttgagcgtga
     2101 cgaacgcggc gatgccttgg ccttttagtt cgtgcggcat gccaacgcac gcggcttcgg
     2161 caacactcgg gtgcgcgacc agcgcgcttt cgatttcggc tgtgccaagg cgatggccgg
     2221 agacgttcaa cacgtcatcc acccgaccca tgatccagaa gtagccgtcg tgatcgcgcc
     2281 gggcgccgtc gccggtgaag tacattccct tgacgtcgga ccaatattga cggacgtagc
     2341 ggtcggggtc attgaagatg ccgcggagca tcgccggcca aggcttgcgg acgacgagca
     2401 gcccgccctg gttggggccg agttcctgac catcgcgcgt gacgatcgcc gcgtcgacgc
     2461 cgaagaacgg cagcgtcgcc gtgccgggtt tggtcggtgt cgccccgggc agcggcgtga
     2521 tcatgatcat gccggtttcg gtctgccacc atgtatcgac gatcggacag cggttgtggc
     2581 cgatcacgtc gcgataccac atccacgctt cggggttgat cggctcaccc acggtgccga
     2641 gcagccgcag cgatgacagg ttgtgcttgt ccaccagctc gcggccggcg cgcatgaacg
     2701 cgcgaatcgc ggtcggcgcg gtgtaaaaga gcgtgacctt gtgccgctcg atgatcgacc
     2761 agaagcgcga gaaatctggg aaattcggcg cgccttcata catcagcacc gtcgcgccgt
     2821 tgctcagcgg gccgtacacg acatagctgt gccccgtgat ccatccgacg tcggcggtac
     2881 accagtagac atcgtcatcg tgcaggtcga acacgtactt ggtcgtgatc gaggtgccga
     2941 ccatgaaccc cgcggtggtg tggatgatgc ccttgggctt gcctgttgat ccgctggtgt
     3001 acagcacgaa cagcaggtct tcagcgtcca tcggctcggc tgggcagtcg gtggattggt
     3061 tggcaatgac atcgttccac cacacgtcgc gcccttcgac ccagttgatg ttcccgcccg
     3121 tgcgacgcag gacgacgacc ttcttgatct tgtccgtctt ggtcagcgca tcatcgacgt
     3181 tcttcttcag cggcacgatg gcgccccggc gatagccacc gtcggcggtg atgaggtagt
     3241 tcgactgcgc atcttccacg cggtcggcga tcgcctggct ggaaaaccca ccgaagatga
     3301 cggagtgcgc ggcgccaatc cgcgcgcacg ccagcatggc gatggccgcc tcggccagca
     3361 tcggcatgta gatcgtcacg cggtcgcccc ggcggatgcc gaggctcttc aaaccgttcg
     3421 ccagccggca gacttcgtct tttagttgct tgtacgacaa tcgccgtaca tcgtgattgc
     3481 cgccctcgga gatcggctcg gcttcgaaga tgatggccgt cttgtcccca cggccctgct
     3541 cgatctgtcg atcgagacag ttgtacgcca cgttcgtctg cccgccgacg aaccatttgg
     3601 cgtgcggcgc tttccactgc agcacttggt cccatcgctt gaaccagtga aagtcgccgg
     3661 cgatctttcc ccagaacggg tcggg
//
LOCUS       DATIMH010000085         4303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1752070, whole genome shotgun sequence.
ACCESSION   DATIMH010000085 DATIMH010000000
VERSION     DATIMH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752070"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VLI90_01465"
     CDS             complement(<1..321)
                     /locus_tag="VLI90_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12898.1"
                     /translation="MPDNRRLNRVFCVGGGRGNCFPQAAGVSFMMPPTPADESAAMKH
                     SDSTATARERAARDFSAIVQAMKELHDIRVKKWRRSMSGCAWRVYHHDGRVVNWIESP
                     VPKTP"
     gene            348..1043
                     /locus_tag="VLI90_01470"
     CDS             348..1043
                     /locus_tag="VLI90_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12899.1"
                     /translation="MTGMMPLAIAATLPRFNFAIFFSLLLVIVASGIVFFFLTHRWTL
                     DRRRAALSDWASEKNFSLRLPPRAQLPAALQSLTSVGARVNLAFTRGPISILQLTTIA
                     QPNSRELRWHVLLREMDRAWSPAGLRPINPAPSFLDLFALNGFPSLLPPERFSVFATE
                     PSAARAMAKSPVRGLLPPDIGLLVHGPYVTLDFSRRPFDTIEFERMLVVMEQLVRHLP
                     AQAEAATSRAEKD"
     gene            1202..2044
                     /locus_tag="VLI90_01475"
     CDS             1202..2044
                     /locus_tag="VLI90_01475"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HSV12900.1"
                     /translation="MDDVRVNSWNELNDRLFAGAWQEPMGRFRSTFAFRGVSLADVDL
                     TTTLIRLGEPFGRQEGHLLRNFRKYATRDAVPGESVWNWLALAQHHGLPTRMLDWTFS
                     PFVAMHFATMDSDDFDRDGAIWCVDYVKVHSLLPRELKKVLKDEDADVFTAEMLSRVA
                     GTLPEFDALSGKDDFTVFLEPPSLDDRIVNQFALFSMMSSPVVRLHDWLADRPGLGFR
                     IILPAALKWEVRDKLDQANITERVLFPGLDGLSRWLKRYYTPKKPTTDADRHPSGEGD
                     ESHR"
     gene            2308..2898
                     /locus_tag="VLI90_01480"
     CDS             2308..2898
                     /locus_tag="VLI90_01480"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HSV12901.1"
                     /translation="MANQPPSNSPASAAAAAAPPLVYSSRPASIAAAPLVAVASWVLP
                     GAGYVLIGQRARGLTIGVTIIVLFLLGLLIAGVRVMEVPGYDANGKAIEVPMRDLSTG
                     AVTSHWVMGISPLVEVRSKPWIVPQLLTGPIAIGAAGWSVYESRPDPNNPTQSRGDQS
                     HARVNEIGSLYLSVAGLLNLMAIIDAAHRAAQRAGA"
     gene            2895..3140
                     /locus_tag="VLI90_01485"
     CDS             2895..3140
                     /locus_tag="VLI90_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12902.1"
                     /translation="MINFLADYNYTRPFLQPMPIWNYWYLLLLPLCVGVAVVYKAIKC
                     RSMSQVPAEAASITVWILVCFAAAGVALVGLVKVMSR"
     gene            complement(3264..4019)
                     /locus_tag="VLI90_01490"
     CDS             complement(3264..4019)
                     /locus_tag="VLI90_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12903.1"
                     /translation="MSAAATTALHFSAPDGSRCVQCGYPLRGLDSNRCPECGRAFDPH
                     DRSSMDCGTLGERIIRWLLQPDGWITWISACIAFGWTLLCMLGPMIVPRGIVIATILW
                     AIVYSLRLARFIARWPIVMLCGQDASWLKAGARQGWYITAMFAASALLFAAHQPNRFS
                     FCINDALLERSANHLWNEVPASSAVIRGARIGIIDVGYGCAGGRGVDYYVIAMTTSGE
                     QTVHWMWRPEQNPSEPLPSGMKHLGGKWYQTEW"
     gene            complement(4092..>4303)
                     /locus_tag="VLI90_01495"
     CDS             complement(4092..>4303)
                     /locus_tag="VLI90_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12904.1"
                     /translation="TKKVRMNFSTDNPNWPKVPFIHELWQGAKQELMQIQVRGTVKDP
                     KVSAASLHTFTTTVDEVFSGSGREK"
BASE COUNT          762 a         1441 c         1270 g          830 t
ORIGIN      
        1 cggcgtcttc ggcaccggcg attcgatcca gttcaccacg cgaccatcgt ggtgataaac
       61 gcgccaggcg cagccgctca tgctgcgacg ccacttcttg acgcggatgt cgtgcaattc
      121 tttcatcgcc tgcacgatcg ccgaaaaatc acgcgcggcg cgttcacgcg cagttgcagt
      181 tgaatcactg tgcttcatcg cggctgattc atcggcaggc gtgggcggca tcatgaaaga
      241 cactccagca gcctgaggga agcaattacc gcgcccgccg ccaacacaga aaacgcggtt
      301 gagccgccgg ttatcgggca acgcatttta tgcgcgacga ccatagaatg accggcatga
      361 tgccgctggc gatcgcggcg acgctgccgc gattcaattt cgcgatcttt ttttcgctgc
      421 tcctggtgat cgtcgccagc ggtatcgtgt tcttcttcct gacgcaccgc tggacgctgg
      481 accgccggcg cgctgcgctg tcggactggg catcggagaa aaacttctcg ttgcgcctgc
      541 cgccgcgcgc gcagctgccg gcggcgctgc agagcctcac gtccgtcggc gcccgcgtga
      601 atctcgcgtt tacccgcggg ccgatctcga tcctgcaact gacgacgatc gcgcaaccga
      661 actcgcgcga actgcgctgg cacgtcctgc tgcgcgagat ggaccgcgcc tggtcccccg
      721 ccggccttcg gccgatcaat cccgcgccgt ccttcctcga cctcttcgcg ctcaacggct
      781 tcccctcgct cctgccgccc gagcgcttct ccgtcttcgc caccgaaccc agcgccgccc
      841 gcgcgatggc caagtctccc gtgcgcggcc tgctcccgcc ggacatcggc ctgcttgttc
      901 acggccccta cgtcacgctc gatttttccc gccgcccgtt cgacacgatc gagttcgaac
      961 gcatgctcgt cgtgatggag cagttggtca ggcatttgcc cgcgcaagcc gaggccgcca
     1021 cgtcgcgcgc cgagaaagat taatttccgc gcagagacgc aaaggccgca gagatcagat
     1081 tagagactct ggccctaact ctgcggcctg ccgcgtctct gcgttcaagt ctcattgatc
     1141 gtttgcgtcg aatcacatcg tccgtttctt aaggctgtgc ggccgtgata tcctcaacgg
     1201 cgtggacgat gttcgcgtca acagttggaa cgagttgaac gaccgcctct tcgccggcgc
     1261 ctggcaggaa ccgatgggcc gcttccgcag caccttcgcg ttccgcggtg tgtcgctcgc
     1321 tgacgttgac ctcacgacca cgctcattcg tcttggcgaa ccgttcggcc ggcaggaagg
     1381 ccacctgctg cgcaacttcc gcaagtacgc gacgcgcgac gccgtgcccg gcgagtccgt
     1441 gtggaactgg ctggcgctgg cgcagcacca cggcctgccc acgcgcatgc tggactggac
     1501 cttctcaccc ttcgttgcga tgcattttgc gaccatggac tccgacgact tcgatcgcga
     1561 cggcgcgatc tggtgcgtcg attacgtcaa ggtgcacagc ctgctgccgc gcgagctgaa
     1621 gaaggtgctg aaggacgaag acgccgacgt cttcaccgcc gagatgctct cgcgcgtcgc
     1681 cggcacattg ccggagttcg acgcgctcag cggcaaagat gacttcaccg tcttcctcga
     1741 accaccgagc ctcgacgatc gcatcgtgaa tcagttcgca ctcttttcaa tgatgtccag
     1801 ccccgtcgtc cgcctgcacg actggctcgc cgaccgcccg ggcctgggct tccgcatcat
     1861 cctgccggcc gccctgaagt gggaagtgcg cgacaagctg gaccaggcaa acatcaccga
     1921 acgcgtactg tttcccggct tggacggatt gagccggtgg ctcaagcgct attacacgcc
     1981 gaagaaaccg acgaccgacg cggatcgcca tccgtcagga gaaggcgatg aatcacatcg
     2041 ctaacgcgct acccatgtag ccgcgacgcc tgcgtcgccg ctggccaaac ggcgacgcgg
     2101 gcgtcgcggc tacatcattt tcacctggcc gcccgcgcct gcgctttttc ttccgctatc
     2161 ttcaacgacc ctgtccaaca cacgcgatga tctctcgcgc cgtcgcctcg cgcgttttgt
     2221 tgctcgcaca gcacgcgccg ctcgcccgcc gcgcccgcat ccccgccttt gatccgtttc
     2281 ccgataacga atacagtatc gccccctatg gcgaaccagc cgccgtcaaa ttcacccgca
     2341 tccgccgccg ccgccgctgc gccgccgctg gtttactcgt cgcggccggc gagcatcgcc
     2401 gccgcaccgc tggtggcggt cgcttcgtgg gtcttgcccg gcgccggata tgtcctgatc
     2461 ggccagcgcg cccgcgggtt gacgatcggc gtgaccatca tcgtgctgtt cctgctcggc
     2521 ctgctgatcg ccggcgtgcg cgtgatggag gtgccgggct acgacgccaa cggcaaggcg
     2581 atcgaggtgc cgatgcgcga cctgtccacc ggcgccgtca catcgcattg ggtgatgggg
     2641 atctcgccgc tggtggaggt ccgcagcaag ccgtggatcg tgccgcagct gttgactggg
     2701 ccgatcgcca tcggcgccgc ggggtggagc gtgtacgaat cgcgcccgga tccaaataat
     2761 ccgacgcagt cgcgcggcga ccaatcgcac gcgcgcgtga acgagatcgg ttcgctgtat
     2821 ctctcggtcg ccgggttgtt gaacctgatg gccatcatcg acgccgccca ccgggcggcg
     2881 caaagggcgg gggcgtgatc aatttcctgg ccgactacaa ctacacgcga ccgttcctgc
     2941 agccgatgcc catctggaat tactggtatc tgctactatt acccttatgc gtgggcgtgg
     3001 cggtggtcta caaggcgatc aagtgccggt cgatgagcca agtccccgcc gaggcggcgt
     3061 ccatcacggt gtggatttta gtttgcttcg cggcggcggg cgtggcgctg gtggggttgg
     3121 tgaaggtgat gtcgcgctaa ataacatgcc gcaattatgg cgcatccgct cggggctaaa
     3181 cgcccgctcg gcccttcggg ccgccgttaa aagagacatt cgaaaatcgt gcgctgtgat
     3241 ttagaagcgc gcgcatagat cggctaccac tccgtctgat accatttccc gccgagatgt
     3301 ttcatccctg atggaagcgg ttcggacggg ttttgctccg gacgccacat ccaatgcaca
     3361 gtctgttcgc cggacgtggt catcgcaatg acgtagtaat cgacgcctcg cccgccggcg
     3421 cagccgtaac caacatctat aatcccaatg cgggcgcccc gaatgaccgc cgacgaagcg
     3481 ggaacctcgt tccacaaatg attagccgac cgttcaagca aagcatcgtt gatgcagaat
     3541 gaaaatcggt tcggctgatg cgcggcaaag agcaatgccg aggcggcgaa catcgcggtg
     3601 atgtaccaac cttgtcgagc gcccgccttc aaccaactgg cgtcctgccc gcatagcatg
     3661 acgatcggcc acctcgcgat gaagcgcgcc aatcgaaggg aatagacgat ggcccagagg
     3721 atggtggcga tgacaatgcc acgaggcacg atcatcggcc ccagcatgca gagcaaggtc
     3781 cagccgaacg cgatgcatgc cgaaatccag gtgatccaac cgtctggctg tagcaaccag
     3841 cggatgatgc gctcgccgag cgtgccgcaa tccatcgatg agcgatcgtg tggatcgaac
     3901 gcacggccac attcaggaca tcgattggag tcgaggccac gaagcggata tccgcactga
     3961 acgcagcggg acccatctgg cgctgagaaa tgcaaagctg tcgtcgcggc agcgctcatt
     4021 gcaccacatc cgagccccgc atgtacatgc ggggcttctg tggaagttgc agtgtcgccg
     4081 ttgccgggga attacttctc gcgcccgctg ccgctgaaga cttcatccac cgttgtcgtg
     4141 aacgtgtgca atgacgctgc cgacaccttc gggtccttca ccgtgccacg cacctggatc
     4201 tgcatcagct cctgcttggc cccctgccac agctcgtgga tgaacggcac cttcggccag
     4261 ttcgggttat ccgtgctgaa attcatccgc acttttttcg tgc
//
LOCUS       DATIMH010000086         5585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1752276, whole genome shotgun sequence.
ACCESSION   DATIMH010000086 DATIMH010000000
VERSION     DATIMH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752276"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="VLI90_01500"
     CDS             complement(<1..1354)
                     /locus_tag="VLI90_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HSV12905.1"
                     /translation="MSCGRDARGQLVSPGSGKQWSTTWNDEFNAGAPDLTGFTYDVGN
                     GGWGNSELENYTAPPGSPAPQPNPSGLGSFTQPATGTNSNNVFASTDGTGLGALTIRA
                     IATGSGGSQTYTSGRIKTPNLFNQAYGLIEFRAKLPAGTGLWPAVWMLPANAAGQTSP
                     KYGGWPTSGEIDILESKGQQASLVQGSLHSGSSVNTHITQTQTFSGSGLEPGGFSTTD
                     WHTYDLQWTQGSTNVAGSLKWYVDGVLYETQSGGWFVPSSAPAGDKDAPFDQPFYMLL
                     NMAVGGSYVGSPSLAAGNYDMQVDFLRAYSSITSSASPSWKNNASGNWSAAGNWVSAV
                     VPNASGAAAVFGNILTQPRTVTVDAPVTVGSMSFDSSSGYTVGGTQAITLNSSSAPTV
                     TVVSGNHTISAPITFGKSTTFNIAPANGSLNITSSLSGSVTLTKSGAGMLQAKSLLVT
                     G"
     gene            complement(1472..2434)
                     /locus_tag="VLI90_01505"
     CDS             complement(1472..2434)
                     /locus_tag="VLI90_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV12906.1"
                     /translation="MSAHHRTYRRIRAFTLVELLVVIGIIAVLIGLLLPALNKARQQA
                     NLTACMSNLRSIGLAINMYTTANKLSLPFGYWDGSVPLGTAPDFTKATDWRALLQATL
                     TRQGGQTYVESSNAGGDTSKIATDLFVCKDVPENVGVLTYACHPRLMPAIELKDPVFF
                     FTGKGDFMRPYKITQIKHGAEILLVADSTLKPLPDPGFIHGKMQANFGLGGIDLNAFG
                     GANTTNRSLLLDEGRPATATPVANRDTSIDVLASNGPAFTNKDSMNQNDAVGSSNWGN
                     IRFRHLSDTAANVLMVDGHVETHRLSSDKLHSTLKRLNINVNSK"
     gene            complement(2448..4175)
                     /locus_tag="VLI90_01510"
     CDS             complement(2448..4175)
                     /locus_tag="VLI90_01510"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV12907.1"
                     /translation="MSKKRAIVAAAAATAAVGFTATQPAFALVQTYSTASGAGGKGID
                     HPGFGFFQFDPGQWDSGLDAATTHPPAGEFAFGGSDGSFHEEVHTLLNVGVTGRTGDH
                     AAFFEMNYNPNPPTTSFGSFWFGGVGNDIGPYPKLNGVPVPLNQVMVWGDVLAPAGKP
                     MEFRSESAFTSVGNGFKFGYTGTGGWQTVGGLLSAATAYGTFNYNQAQLSVLSAFGQN
                     GNEITAVDDGSDPNNVPTEMVDNLCLTIASANWGANTTGNWGDNTQWNISAPDGPNAT
                     AVFGGTIAGAAAVNMDSQHFIGTIQLNSAQSYTIGGAGTLSLRSFNGTTFAPIQSSID
                     VVQGNHTISAPVELWGGVNINVEQAGSTLTISNLQPTFIAGAGVDKNGPGTLVVNNVR
                     ADSLGVHNGKVQVIAGAAPNSPAGTTVVKALTIDAGATLDLTNNSMIIDYSPLTVLPA
                     STIRTMLQSGRLTTSTLAAGLKLGYGDNAVLGKTSFAGQDNIDSTTLLIKYTYAGDAT
                     LDGKVDVGDLGALATNYNAGVGSPLVADAAASIAAVPEPASIGLFLFGFAAAATSRRR
                     RCAAAAHHA"
     gene            complement(4404..5444)
                     /locus_tag="VLI90_01515"
     CDS             complement(4404..5444)
                     /locus_tag="VLI90_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSV12908.1"
                     /translation="MSSVRQIARRTRVSVATVSRALNNHAEISPETRERVLRAANEVG
                     YYGTVGKKVTTNIGLVFTSDASFTEFDALIMAGMLRGVAEQRFDVTVVNLERDKAAGE
                     THTQFFMRKGLRGVVLRTFTRSRRICQAIADEGFPSIVVAERFDNPDINCIWADSVPD
                     SRRAVEHLIHLGHRRIAFTMNHLRDRDHDDRCAAYQSVLEAAGIEYDPELVVAVPADL
                     AGGKAALNRLMSLPTPPTAIYYADPLACVGAITRAHEMGLKIPEDVSIVGFDDADIRF
                     RIWPVLTAVCQDASQLGFEAALWLTRAIANPQEGPMRKQVNTFFEVHGTTGQPPKRPI
                     RILPDGMRIPAE"
BASE COUNT          966 a         1772 c         1751 g         1096 t
ORIGIN      
        1 cgccagtcac cagcaggctt ttcgcttgaa gcatgcccgc cccgcttttg gtcagcgtga
       61 cgctgccgct cagcgacgac gtgatgttca gcgaaccgtt ggcgggcgcg atgttgaacg
      121 tcgtgctttt tccgaacgtg atcggagcgc tgatcgtgtg attaccgctg acgacggtga
      181 cggtcggggc gctggatgaa ttgagcgtga tcgcctgggt cccgccgacc gtgtagccgg
      241 acgaactgtc aaacgacatg ctgccgaccg tgacgggggc atcgacggtc accgtgcgcg
      301 gctgcgtgag gatgttgccg aacaccgcgg cggcgccgct ggcgttgggc accaccgcgc
      361 tcacccaatt gcccgcggcg ctccagttac cgctggcgtt gttcttccac gatggcgagg
      421 cgctgctggt gatcgacgaa tacgcccgca agaaatcgac ttgcatgtcg tagttccccg
      481 cggccaggct gggcgaaccg acgtagctcc cgccgaccgc catattcagc agcatgtaga
      541 acggctggtc gaacggcgcg tctttgtcgc cggcgggcgc gctgctgggg acaaaccacc
      601 cgccgctttg agtttcgtac agcacaccgt caacgtacca ctttagcgat ccggcgacgt
      661 tggtgctgcc ttgggtccat tgaaggtcgt aggtgtgcca atcggtggtg ctgaagccgc
      721 ccggttccag gccggacccg gagaatgtct gcgtttgcgt gatgtgcgtg ttgacgctcg
      781 acccgctgtg cagactgcct tggacgagcg aagcttgctg ccccttggat tctagaatat
      841 cgatctcacc ggaggtcggc cagccgccgt atttcggcga ggtttgaccg gccgcgttgg
      901 ccggcagcat ccacaccgcg ggccagagtc cagtgccggc gggcaacttg gcgcgaaact
      961 cgatcaagcc atacgcctgg ttgaacaagt tcggcgtctt gattcgtccc gaggtgtacg
     1021 tctggctgcc accgctgccg gtggcgatgg cgcgaatcgt cagcgcgccc agtcccgtgc
     1081 cgtcggtgga tgcgaagacg ttgttcgagt tggttcccgt cgctggttgc gtgaaacttc
     1141 caaggccgct gggattcggt tgcggcgccg gcgagccggg tggcgcggtg taattctcca
     1201 gctccgagtt cccccaaccg ccgttgccca cgtcgtacgt gaagccggtc aggtcgggcg
     1261 cgccggcgtt gaattcatcg ttccacgtgg tggaccactg cttgcccgag ccgggcgaaa
     1321 ccagctggcc gcgggcatcg cggccgcaac tcaaaaacgc tgtggccgcg ctaccggcaa
     1381 tggcgcagag cagcctagcg cggctaattc ggcgggtggt gaaaaacatg gtgctgagat
     1441 ctccgccact gatgagttac cccaacacta actacttcga gtttacgttg atgttcaggc
     1501 gcttcagggt cgaatgaagt ttatccgagc taagccggtg cgtttccaca tgcccgtcga
     1561 ccatcagcac attcgcggca gtgtcgctca gatggcggaa tcggatgttg ccccaattgg
     1621 aactgccgac ggcatcgttc tggttcatcg agtctttgtt ggtgaacgcc ggaccgttgg
     1681 acgccagtac atcaatggac gtgtcgcgat tggccaccgg agtggcggtg gcgggcctgc
     1741 cctcatccag cagcagcgat cgattggtgg tattggcgcc gccgaacgcg ttcagatcaa
     1801 tcccgccgag gccgaagttg gcctgcatct taccgtggat gaagccggga tccggaaggg
     1861 gtttgagcgt tgaatctgcg acgagcagaa tctccgcgcc atgcttgatc tgggtgatct
     1921 tgtacggccg catgaaatcg cccttgccgg tgaagaagaa gacgggatcc ttcaactcga
     1981 tggcaggcat gagtcgcgga tggcatgcgt aggtcagcac acccacgttc tccggcacgt
     2041 ctttgcacac gaacaaatcc gtcgcaatct tggacgtgtc cccgccggcg ttggagcttt
     2101 cgacgtacgt ttgcccgccc tgcctggtga gggtcgcctg aagcagcgcg cgccagtcgg
     2161 tcgccttggt gaagtcgggc gcggtgccca gcggcactga gccgtcccag tagccaaagg
     2221 gcaggctaag cttgttggcg gtcgtataca tattgatcgc cagcccgatc gaccgcaggt
     2281 tggacatgca cgcggtcagg ttcgcctgct gccgcgcctt gttcagcgcc ggcagcagga
     2341 gcccaatcag caccgcgatg atgcctatca cgaccagcag ctccacaagc gtaaacgcgc
     2401 gaatgcgacg atatgtgcga tggtgagcgc tcattgcaga cactccttca cgcgtgatgt
     2461 gccgcggcag cgcagcgccg gcggcgcgag gtggcggcgg cggcgaagcc gaagaggaaa
     2521 agcccgatcg aggccggttc aggcacggcg gcgatggatg cggcggcatc ggcgaccagc
     2581 ggtgaaccga cgcccgcgtt gtagttcgtc gccagcgcgc ccaggtcgcc aacgtccacc
     2641 ttcccatcca acgtggcatc cccggcgtag gtgtacttga tcagcaaggt ggtcgagtcg
     2701 atgttgtcct gtcccgcgaa cgacgtcttg cccaacaccg cgttgtcgcc gtaacccagc
     2761 tttagcccag ccgccagggt gctcgtggtc aaccggccgc tttgaagcat cgtgcggatc
     2821 gtgctcgccg gcagcaccgt cagcggcgag taatcgatga tcatcgagtt gttcgtcaga
     2881 tcgagcgtcg cgccggcgtc gatcgtcagc gccttcacga ccgttgtccc agccgggctg
     2941 ttgggcgcag cgccggcgat cacctgcacc ttgccgttgt gaacgccaag gctgtcggcc
     3001 cgcacgttgt tcaccaccag cgtgccgggg ccgttcttat cgacgcctgc gccggcgata
     3061 aacgtcggtt gcagattgga aatcgtcaac gtagacccgg cctgctcgac gttgatgttc
     3121 acgccgcccc acagctcgac cggcgcgctg atcgtgtgat tgccttgcac gacatcgatc
     3181 gaactttgga tcggcgcgaa ggtcgtgccg ttgaagctgc gcaggctcag cgtgccggcg
     3241 ccgccgatcg tgtacgactg cgcgctgttc aactggatcg tgccgatgaa atgctggctg
     3301 tccatgttga cggccgcggc gccggcaatc gtgccgccga agaccgccgt cgcattcggc
     3361 ccgtccggcg ccgagatgtt ccactgcgta ttgtcgcccc agttgccggt cgtgttcgcg
     3421 ccccagttgg cggaggcgat cgtcaggcag aggttgtcga ccatttcggt ggggacattg
     3481 tttggatcgg aaccgtcatc gaccgcagtg atctcgttgc cgttctggcc gaacgccgac
     3541 agaaccgaga gctgcgcctg gttgtaattg aacgtgccgt aggcggtcgc ggcagacagc
     3601 agtccgccca cggtttgcca cccgccggtc ccggtgtagc cgaacttgaa accattaccg
     3661 acactggtga atgcgctttc gctgcggaac tccatcggtt tccccgcggg cgcgaggacg
     3721 tcgccccaca ccatcacctg gttcagcggc accggcacgc cgttcaattt cggataggga
     3781 ccgatgtcat tgcccacgcc gccgaaccag aacgagccga agctggtggt gggcgggttg
     3841 gggttgtagt tcatctcgaa gaacgccgcg tgatcgccgg tgcgaccggt gacgccgacg
     3901 ttcaggagcg tgtggacctc ctcgtggaaa ctgccgtccg aaccgccaaa cgcgaattcc
     3961 cccgccggtg gatgcgtcgt ggcggcatcc aggccgctgt cccactggcc tggatcgaac
     4021 tgaaagaacc cgaatcccgg atggtcgatt cccttgccgc ccgcgccgct ggcagtgctg
     4081 taggtttgca ccagcgcaaa cgccggctgc gtcgccgtga acccaaccgc tgccgtcgcc
     4141 gctgccgccg cgacaatcgc tcgtttcttt gacatgctcc tcatcgcact tcctccgaaa
     4201 tgatggacgt gtttctcccg cgttcttctc ttttttcgtt cacagccacg aaccctcagg
     4261 cggctccgct gcgcgtcctc tgggcgttcg ccgcgcgcga tctcgcgagc cacaatcttc
     4321 aactgacatg aaagcgatcc gtgcgtttac tccgctccgc tccaactcgc gcgactgtgc
     4381 cacaggcgcg cccgcttact cgctcattcc gccgggatcc gcatcccgtc gggcaaaatg
     4441 cggatcggcc gcttcggcgg ttgaccggtc gtgccgtgca cttcaaaaaa tgtgttcacc
     4501 tgtttccgca tcgggccctc ctgaggattg gcgatcgccc gcgtcagcca cagcgccgct
     4561 tcgaagccca actgcgacgc atcctggcac accgccgtca gcaccggcca aatgcggaag
     4621 cgaatgtccg cgtcgtcaaa cccgacaatc gaaacgtctt cgggaatctt caaacccatc
     4681 tcgtgcgcgc gggtgatcgc cccgacgcac gcgagcgggt cggcgtaata aatggctgtc
     4741 ggtggcgtcg gaaggctcat caaccgattg agcgccgcct tcccgccggc caaatccgcc
     4801 ggcacggcaa caaccaattc cgggtcatac tcaattcccg ccgcctcgag caccgactga
     4861 tacgcggcac agcgatcgtc gtgatcgcgg tcgcgcaggt ggttcatcgt gaacgcgatc
     4921 cgccggtggc ccaggtgaat caggtgctcc accgcccgcc ggctgtccgg gaccgaatcc
     4981 gcccagatgc agttgatgtc gggattgtcg aaccgctcgg ccacgacgat ggacgggaaa
     5041 ccctcatccg caatcgcctg gcaaattcgc cgcgaccgag tgaacgttcg cagcaccacg
     5101 ccgcgaagcc ccttgcgcat gaagaactgc gtgtgggttt cacccgcggc cttgtcgcgt
     5161 tcgaggttga cgacggtgac gtcgaaccgc tgctcggcca cgccgcgcaa catgccggcc
     5221 atgatcaacg cgtcgaattc ggtgaagctc gcgtcgctgg tgaagacgag cccgatgttg
     5281 gtggtgactt ttttcccgac ggtgccgtag tagcccactt cgttggccgc gcgcaagacg
     5341 cgctcgcggg tctcggggct gatctcagcg tggttgttca aggcccggga gacggtggcg
     5401 accgaaacgc gggttcgacg tgcgatttgg cggacagaag acatctgcac cagcccttcg
     5461 taacagttgc cgtaacatta ccgggactgt tacggccgtg tcaacaatat tccaaaatcc
     5521 tcatcgcatt acgaaaaatg gctgatcttg cggtctgtag ctgcgacgcc tgcgtcgcct
     5581 gtttg
//
LOCUS       DATIMH010000087         3998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_885900, whole genome shotgun sequence.
ACCESSION   DATIMH010000087 DATIMH010000000
VERSION     DATIMH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885900"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VLI90_01520"
     CDS             <1..442
                     /locus_tag="VLI90_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12909.1"
                     /translation="VIAIRVEAAHPLDVDDREAFVHTLSDEGADVPYVMFVDQENTRL
                     YEQKHGKLAGPTVESPTHAILSLYDSSFGSGPVYQPYMEVLVESWIRDLLLHWKSPDP
                     PLSQEMGALFSSRLTPGFTASASSSDASSSSSTSSSSSSSNGGW"
     gene            446..1045
                     /locus_tag="VLI90_01525"
     CDS             446..1045
                     /locus_tag="VLI90_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12910.1"
                     /translation="MIAYLETNFLLAAATGQDAQTSALLQTDRANAQLFMPDVCVMEG
                     WYAIVGKRKEFAAFRAELDRRVREAQRDRTSTMAPSILPSLLDAQLRSGALLNDIERR
                     FSDTLSAFVVRGELLPLLPIALDTSLKQRHIDAPADNLILHVICEHAARHHTTDRVLL
                     TANTNDFASSHVRNVLRAYGVTSLVSDCSNLTRQINAII"
     gene            1054..1842
                     /locus_tag="VLI90_01530"
     CDS             1054..1842
                     /locus_tag="VLI90_01530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV12911.1"
                     /translation="MVISSSGAALITGASSGIGYEFARLCAADKRSLIVTARNAESLK
                     QLAAESREAHGVGVDVIPADLATNAGAKDLVGDLERRNITVDILINNAGFGTHGKFWE
                     NDVEQEQALLHVNVVSLMLLTRLLLPGMVARGRGRILNVASTAAFVPGPLMANYYASK
                     AYVLSLSESLSSELRGTGVTVTALCPGPTATAFQSRAGVKPSKLADVAGMSAADVARV
                     GYDAMMAGKRVAIPGVKNRLLVGLSRFAPRVALLGFVRKLNESR"
     gene            1850..2503
                     /gene="pdxH"
                     /locus_tag="VLI90_01535"
     CDS             1850..2503
                     /gene="pdxH"
                     /locus_tag="VLI90_01535"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HSV12912.1"
                     /translation="MTQPPRPDPAGIRQEYTLGELVESAVSPDAIEQFGRWFNDAIAA
                     KLPEPTAMTLATADATGRPDARIVLLKAFDQRGFAFYTSYISRKGRELAENPRACLVF
                     FWEALQRQVRIDGRAEFVSREETEAYFHSRPVKSQVGAWVSKQSRVLSSRGELEKRAA
                     ELFVQFAGGEIPVPEFWGGYRVVPQEIEFWQGRRSRLHDRLRYTRAGDAWKIERLWP"
     gene            2669..3052
                     /locus_tag="VLI90_01540"
     CDS             2669..3052
                     /locus_tag="VLI90_01540"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HSV12913.1"
                     /translation="MTDTRAFTFTGRLRSVRYAVRGVRLMLASQHNAWVHAAATVLVT
                     AAGLIARLDRAEWCWLVAAMMAVWTVEAINTAFEFLCDVASPDFHPLVEKAKDVAAGV
                     VLLSAIGSVIVGLLIFAPHVMVHLR"
     gene            3052..3462
                     /locus_tag="VLI90_01545"
     CDS             3052..3462
                     /locus_tag="VLI90_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12914.1"
                     /translation="MLLSESLGVAELTDQQVYSTIGEVGFTALVAAFYRRVPTDDLLS
                     HLYPPDDFDAAERRLREFLIQRFGGPARYSETRGHPRLRMRHAPFKVDRAARDRWMTL
                     MDAALVETQLPADVTFLLRKYFDDTATFMINHHT"
     gene            complement(3588..>3998)
                     /locus_tag="VLI90_01550"
     CDS             complement(3588..>3998)
                     /locus_tag="VLI90_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12915.1"
                     /translation="IKGGNGSNIIVGGAGDDVLVGGSGRDIVIGGAGSDALVTGGGDD
                     VLVAMSTQYDNNATALESIQAEWTRSDLNFTDRANHLLHGGGLNGSNILSSATLVNDN
                     ATDVLAGGGGDDLFIISGGDIADANKVDLTWVNM"
BASE COUNT          703 a         1251 c         1262 g          782 t
ORIGIN      
        1 cgtgatcgcg attcgagtag aggccgcgca tccactggat gttgacgatc gggaggcttt
       61 cgttcacacc ttgtccgatg agggcgcgga cgtgccgtac gtgatgttcg ttgaccagga
      121 aaacacgcgg ttatacgagc aaaagcatgg gaagctggcc ggcccaacgg tggagtcccc
      181 aacccacgcg atcctttctc tttatgactc gtcgttcggc agcggcccgg tttatcagcc
      241 gtacatggag gtgctggtcg agtcttggat tcgagatctg ctgctgcatt ggaagtcgcc
      301 cgatccaccg ctgtcacagg aaatgggagc gttgttctca tcgcgattga cgcccggatt
      361 taccgcctcg gcatcatcgt cggatgcttc gtcgtcatct tccacgtcca gttcgtcaag
      421 ttcgtccaac ggcggttggt gacgcgtgat tgcgtacctc gagacgaact tcctgcttgc
      481 agctgcaacg ggtcaggatg cacagacgag cgcgctgctg cagacggatc gcgccaacgc
      541 tcagctgttc atgccggatg tttgcgtcat ggagggctgg tacgccatcg tcggcaagcg
      601 aaaagagttc gcagcctttc gcgccgaact ggatcggcga gtgcgcgagg ctcagcgcga
      661 tcgcacctct acgatggctc cctcgattct tccatcgcta ctggatgcgc aattgcgcag
      721 tggagcgctg ctcaacgaca tcgaaaggcg tttcagcgac acgctgtcgg ctttcgtagt
      781 gcgaggtgaa ttgctgccgt tgctcccgat cgctcttgat accagcctaa agcagcgcca
      841 catcgacgcg ccggcggata atcttatcct ccacgtgatc tgcgagcacg cggcacggca
      901 tcatacgacc gatcgcgttc ttcttaccgc caacacgaac gacttcgcct cgtcgcacgt
      961 tcgcaacgtg ttgcgcgcgt atggcgtgac cagtctcgta agcgattgca gcaatctgac
     1021 ccgtcagatc aacgccatca tctgagatcg aaaatggtta tctcctcgag tggcgcagct
     1081 ttgatcactg gcgcgtccag cggcatcgga tatgaattcg cgcgcctttg cgccgctgat
     1141 aagcgatcgc tcattgtcac ggcgcgaaat gccgaatcat taaagcaact ggctgccgaa
     1201 agccgtgaag cgcacggcgt cggcgttgat gtgattccgg cagacctggc taccaacgcc
     1261 ggagctaaag atctcgtcgg cgacctcgaa cgccggaaca tcaccgttga catcctcatc
     1321 aataacgccg gcttcggcac gcatggaaaa ttctgggaga acgatgttga acaggagcaa
     1381 gcgttgctcc acgtgaacgt cgtctcgctg atgctgctca cgcggctgtt gttgccgggg
     1441 atggtcgcgc gcgggcgcgg gcggattctc aacgtcgcct cgacggcggc gtttgtgccg
     1501 gggccgttga tggccaacta ttacgcgagc aaggcgtacg tgctgtcgct gtcggagtcg
     1561 ctgtccagcg aactgcgcgg caccggggtg accgtcaccg cgttgtgccc gggtccgacg
     1621 gcgacggcgt tccagagccg cgcgggcgtc aagccttcaa aactggccga tgttgccggc
     1681 atgtcggcgg cggacgtcgc ccgcgtgggc tacgatgcga tgatggccgg caaacgcgtc
     1741 gcgatccccg gcgtgaaaaa ccgcttgctc gtcggcctct cgcgcttcgc gccgcgcgtg
     1801 gcgttgctgg ggttcgtgcg taagctgaac gaatcgaggt gaagacgcga tgacgcaacc
     1861 cccccgaccc gaccccgccg gcattcggca ggaatacacg ctcggcgaac ttgttgaatc
     1921 cgccgtctcg cccgatgcga tcgagcagtt cggccggtgg ttcaacgatg cgatcgcggc
     1981 gaaattgccg gagccaaccg caatgacctt ggcgaccgcc gacgccacgg gcaggcccga
     2041 tgcgcggatt gtgttgttaa aagcattcga ccagcgcggc ttcgcgttct acaccagcta
     2101 catcagtcga aagggccgcg agctggccga gaacccgcgg gcgtgcctgg tgtttttctg
     2161 ggaagcgctg cagcggcagg tgcgcatcga cggccgggcc gagttcgtca gccgcgaaga
     2221 gactgaagcg tatttccatt cgcgcccagt gaagtcgcag gtcggcgcat gggtgtcgaa
     2281 gcagagccgc gtgctctcat cgcgcggcga gctggaaaag cgcgccgccg aactgttcgt
     2341 ccaattcgcc ggcggcgaaa tcccggtccc ggaattctgg ggcggctacc gcgtggtgcc
     2401 gcaggagatc gaattctggc aggggcggcg aagccggttg cacgatcgat tgcgctacac
     2461 acgcgccggc gatgcctgga agatcgaacg actgtggccg tgaatataca gccggaggcg
     2521 cgagcgcgca tttcgatcga acgtgagatt cggttacagc cgcacacgac agccagcggg
     2581 cgacagccag cgggcgcgct cgaatctcca gccgcttcgc attcgcacgc actcgctggc
     2641 tgtcgcctgc ggctagcaag gccgttaaat gaccgacacc cgcgccttca ccttcaccgg
     2701 ccgacttcgc agcgttcgat acgcggtgcg cggcgtgcgc ctgatgctcg ccagccagca
     2761 caacgcgtgg gttcacgccg ccgcgaccgt gctcgtcacg gccgctggtt tgatcgcgcg
     2821 gctcgatcgc gccgagtggt gctggctcgt cgccgcgatg atggccgtgt ggaccgtgga
     2881 ggcgatcaac accgcgtttg aatttctgtg cgatgtcgcg tcgccggact ttcatccgct
     2941 ggtcgagaag gcgaaggacg tcgccgccgg cgtggtactg ctcagcgcga tcggctcggt
     3001 catcgtcggc ctgctgatct tcgcgccgca cgtgatggtg catcttcgct aatgttgctt
     3061 tcggagagtc tcggcgtggc ggagctgacg gaccagcagg tgtattcaac gatcggcgag
     3121 gtgggattca ccgcgctcgt cgccgcgttc taccgtcgcg tgccgacgga cgatctgctg
     3181 tcgcacctct atccgcccga tgacttcgac gccgccgagc ggcggctgcg cgagttcctc
     3241 atccagcgat tcggcggtcc tgcccgttac agcgaaaccc gcggtcaccc gcggctgcgc
     3301 atgcggcatg caccgttcaa agtcgatcgc gccgcccgcg atcggtggat gacgctgatg
     3361 gatgcggcgc tcgtcgaaac gcagctgccg gccgacgtca cgtttctgtt gcggaagtat
     3421 tttgacgata ccgcgacgtt catgatcaac catcacacgt agcacgggcg tctcgcccgt
     3481 gcctgggcac tcacgcacgg gcgagacgcg gcgagacgcc cgtgctacga aaaaagcccc
     3541 agcgctttcg cgccggggct ttttcaattc caaggatgac tcagcaatta catgttcacc
     3601 caggtcaggt cgaccttgtt cgcatccgcg atatcgccgc cgctgatgat gaacaagtcg
     3661 tcgccgccgc cgccggccag cacgtcggtg gcgttgtcgt tcaccagcgt cgcgctgctc
     3721 aggatgttcg agccgttcaa accaccgccg tgcagcaggt ggttggcgcg atcggtgaag
     3781 ttcagatccg accgcgtcca ctcggcctgg attgattcca gcgccgtggc gttgttgtcg
     3841 tattgggtgg acatggcgac cagcacgtcg tcaccgccgc cggtcaccaa cgcgtcgctg
     3901 ccggcgccac cgatgacgat atcgcggccg ctgccgccga ccagtacgtc gtcgccggcg
     3961 ccaccgacga tgatgttgct gccgttaccg cccttgat
//
LOCUS       DATIMH010000088         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5218614, whole genome shotgun sequence.
ACCESSION   DATIMH010000088 DATIMH010000000
VERSION     DATIMH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5218614"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..481
                     /locus_tag="VLI90_01555"
     CDS             239..481
                     /locus_tag="VLI90_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12916.1"
                     /translation="MKSSRTNWTARRRWLPLAAGVLVILQVLLMYASAAVGATERGAT
                     QSKDLAEKKVNRVHPRRSLALEDDKNDTRRIRKGLP"
     gene            746..1492
                     /locus_tag="VLI90_01560"
     CDS             746..1492
                     /locus_tag="VLI90_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV12917.1"
                     /translation="MDHRVALITGAGRGIGLAMARALAGAGCAVAIQDIDLPVAQTEA
                     ERINAAGGRAIALGGDVLDLNLPARAVKEVVEKLGGLHVLINNAAIQQPRHWLESSAE
                     EMERQLRADLVAPILFCQQVVPIFRRQQWGRIINMGSIQQQKGNPTMLAYSLSKAALE
                     KMTTALARDLGAGQITVNLIAPGWTHTYRTEKDFTSDQDKLEKGKGAAPLGRVGEPTD
                     FEGIVLLLCSDAGAYITGQSIYVDGGMSAR"
     gene            1614..2669
                     /locus_tag="VLI90_01565"
     CDS             1614..2669
                     /locus_tag="VLI90_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HSV12918.1"
                     /translation="MHLQPFVKLADPARYVACAMDLSVDHDARQHWVRFFRKNFDTIL
                     KLGVDAAAARGESREAATDRAKSCRAEFDEIFDAYTASPSAAGRVTMLTIDAWRDDTL
                     RKHGFTDAFIDLKHRENERALPLLPAVCRQIDALRGADQLRAVIEGVFAGNIFDMGSE
                     ATAAAYLHGGPDFFATRASLSPRPWLIDDYDALQRRLLNRPIHRKAVFFIDNAGSDFL
                     LGVAPLARWLAMRGTRVVLAANQTPALNDMTIADVNEWWPRIVEIEPSLKSLPIDRVS
                     TGTAEPLIDLTGVSRELNEASADADLVILEGMGRGIESNLDARFSCAALNIAMIKDAM
                     TAKQLGGKLYDVVCRFR"
     gene            2856..3596
                     /locus_tag="VLI90_01570"
     CDS             2856..3596
                     /locus_tag="VLI90_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HSV12919.1"
                     /translation="MLNFDPKNVDPRTMARAATVVASHGEDRPMPGRGPMAMPMAMPG
                     PPGQGMNRYREMTLEEMLLENRVLFLGGEINHVSASRIIMQMLYLQSVKKDADINLYI
                     NSPGGVVDDTLAIYDIMRFLTCDVATYCIGRAESGGAVVFMAGKKGKRFILPNAKVMI
                     HQPFGGVYGQAADIEIQAEEILKTKQTLINVMAKCTGQTPERIQEDSERDRFFEAKAA
                     VAYGICDEVLEDAASAEAVAATDGNRPK"
     gene            3698..4099
                     /locus_tag="VLI90_01575"
     CDS             3698..4099
                     /locus_tag="VLI90_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSV12920.1"
                     /translation="MLFTGIDHPAMSCQDAKVLADWYCQNLGMKVIGQNDETPPSFVL
                     GWGDRCDDPGGLLELMPIKHPGGPKPTDVPRFCQGLRHFAVRVSNFDQAYAQLKAAGV
                     MFLFEPVQAVGGGKIVSFRDPEGNELQIVER"
BASE COUNT          817 a         1245 c         1414 g          740 t
ORIGIN      
        1 ttgtgttcat cgttttccgt cttagacggc aaggacccgg cgctgcccag tatttacgca
       61 acatttgcag cttcttgcgc ggcgacggga aaaactcgat cgcgcctttg taaagccggc
      121 gatggcatcg cccgggatgg cgcggggacc ggcgatgcca tcgccggctt tacaggaata
      181 gttgtcccgg ttcagggaca tgaaacggcg tcaccggcat tcctagagtc gccgcgatat
      241 gaaaagttca cggacgaatt ggacggcgcg ccggcgatgg ctgccgctgg cggcgggggt
      301 gctggtgatc ttgcaggtcc tgctgatgta tgcgtcagcg gccgtcgggg cgaccgaacg
      361 cggcgcgacg cagtcgaaag atctggccga gaagaaagtg aaccgcgtcc atccgcgccg
      421 gagcctcgcg ctggaggatg acaaaaacga tacacgccgg attcggaaag gcctgccctg
      481 agcctgtcga agggtactcg cgacgaagcg aggttctgcc gcgcgtcgac tagaagcatc
      541 cgggtcgtcg gagtacctcc gaacccggcg tgtgggattc cgcgggcgcc ctctcaccgg
      601 gaagcaccgc ggcgtcgctt catcaacggc gcccgccctc cgtcactaca tatttccact
      661 tccgatcaag cgtttgcaat ggacgcgcta gccgctagca ttccgccggc tgagtatgga
      721 atcgccgcct tccgtgagtt ttcgcatgga tcatcgcgtc gcactgatca ccggcgctgg
      781 ccggggcatc ggcctggcga tggcgcgcgc gttggccggc gcgggatgcg cggtcgcgat
      841 ccaggatatc gatctgccgg tggcgcagac ggaggcggag cggattaacg cggcgggggg
      901 ccgggcgatc gcgctgggtg gcgacgtgct ggacctgaac ctgccggccc gcgcggtgaa
      961 ggaagtcgtc gagaaactcg gcggcctgca cgtgctgatc aacaacgccg ccatccagca
     1021 gccgcggcat tggctggagt cgtcggcgga ggaaatggaa cgccagctgc gcgcggacct
     1081 ggtcgcgccg atcctgttct gccagcaggt ggtgccgatc ttccgccgcc agcagtgggg
     1141 gcggatcatc aatatggggt cgatccagca gcagaagggc aacccgacga tgctggcgta
     1201 ttcgctgtcg aaagcggcgc tggaaaagat gacgacggca ctggcccgcg atctgggcgc
     1261 gggacagatt acggtgaacc tgatcgcgcc gggctggacg cacacttatc gcacggaaaa
     1321 agatttcaca tcggatcagg acaagctcga gaagggcaag ggggccgcgc cgctgggccg
     1381 cgtcggcgag ccgacggatt tcgagggcat cgtcctgctg ctctgcagcg acgcgggggc
     1441 gtacatcacc gggcaatcga tctacgtcga tggtgggatg agcgccaggt aacgcccacg
     1501 ggttcggagg tactccgacg acccggatct tccgcgcgaa gaatccgggt cgtcgcgagt
     1561 acgttccgaa cccggcatgg ggaggcttgg tcacgagatg gagttgttgg cgcgtgcact
     1621 tgcaaccgtt cgtgaagctg gctgatcctg ccaggtacgt cgcgtgcgcg atggacctgt
     1681 cggtagacca cgacgcgcgg cagcactggg tccggttctt ccgtaagaac ttcgatacga
     1741 tcctgaagct gggcgtcgat gccgccgctg cgcgtggtga atcgcgcgag gcggccaccg
     1801 atcgcgctaa gtcatgccgg gcggagttcg acgagatctt cgatgcctac acagcgagcc
     1861 ccagcgccgc cggtcgcgtc acgatgctca ccatcgacgc gtggcgcgac gacacgctgc
     1921 gcaagcacgg cttcaccgac gcgttcatcg acctgaagca ccgcgagaac gagcgggcgc
     1981 tgccgctgct gccggcggtt tgccggcaga tcgacgcgct gcgcggcgct gatcaacttc
     2041 gcgccgtcat cgaaggcgtc ttcgccggca atatcttcga catgggctcc gaagccaccg
     2101 ccgccgcgta tctgcatggc gggccggact tcttcgcgac gcgcgcgtcg ctctccccgc
     2161 ggccgtggtt gatcgacgat tacgacgcat tgcagcggcg gctgctcaac cggccaattc
     2221 atcgcaaggc ggtcttcttc atcgacaacg ccggcagcga tttcctgctc ggcgtcgcgc
     2281 cgctggcgcg ctggctcgcg atgcgcggca cccgcgtcgt cctggccgcc aaccaaacgc
     2341 cggcgttgaa cgacatgacg atcgcggatg tgaacgagtg gtggccgcgg atcgtcgaga
     2401 tcgagccatc gctgaagtcg ctgccgatcg atcgcgtcag cactgggacg gcggagccgt
     2461 tgatcgatct caccggcgtg tcgcgcgagt tgaacgaagc atccgccgac gccgatctgg
     2521 tgattctcga aggcatgggc cgcgggattg agagcaacct cgacgcgcga ttctcgtgcg
     2581 ccgcgctgaa catcgcgatg atcaaggatg cgatgacggc gaagcaactg ggggggaagc
     2641 tgtacgacgt cgtctgccgg tttcggtgat ttcacagccg ctagcggctt agcgtctgtt
     2701 ccaatcgaag gaaagaaaca aacgctaagc cgcaagcggc tgggtttgat tgccactcga
     2761 tcttctcgga cgctcgtccg ttcgttgacg ctcccatacc ccctgcgtac catccgacgc
     2821 tctatattcg acaacggcag accgaggaca cgcgaatgct caactttgat ccgaagaacg
     2881 tagacccgcg gacgatggcg cgggcggcga ccgtcgtcgc cagtcatggc gaagaccgcc
     2941 cgatgcccgg tcgtggaccg atggcgatgc cgatggccat gcctggcccg ccggggcagg
     3001 ggatgaaccg ctaccgcgag atgacgctcg aggaaatgct cctggaaaac cgggtgctgt
     3061 tcttgggcgg tgaaatcaac cacgtcagcg ccagccgcat catcatgcag atgctttatc
     3121 tgcaaagcgt gaagaaagac gcggacatca acctgtacat caattcgccg ggtggcgtcg
     3181 tcgatgacac gctggcgatc tacgacatca tgcggttcct cacctgcgat gtcgcgacgt
     3241 actgcatcgg ccgagccgaa agcggcgggg cggttgtgtt catggcgggc aagaagggca
     3301 agcgcttcat cctgcccaac gcgaaggtaa tgatccacca gccgttcggc ggcgtgtacg
     3361 gccaggcggc cgacatcgaa attcaggccg aggaaatcct caagaccaag cagacgctga
     3421 tcaacgtgat ggctaagtgc accgggcaga ccccggagcg gatccaggaa gattcggagc
     3481 gcgaccgctt cttcgaagcc aaggccgccg tggcgtacgg catctgcgac gaagtgctgg
     3541 aagacgcggc cagcgccgaa gcggtggcgg cgacggatgg gaatagaccg aagtgagtgg
     3601 gtcagttgtc agtggtccgt tgtcagttgt ccgttgaacg caactggccg caagagcaac
     3661 tgacaacgga ccactgacaa cggacaacgt accaaaaatg cttttcaccg gaatcgatca
     3721 tcccgcgatg agttgccagg acgccaaggt gctggcggat tggtattgcc aaaatctggg
     3781 catgaaggtg atcgggcaga acgacgagac cccgccgagt ttcgtgctgg ggtggggcga
     3841 tcgatgcgat gatccaggcg ggctgctgga actgatgccg atcaagcatc ccggcgggcc
     3901 gaagccgacc gatgtgccgc ggttttgcca gggccttcgg cacttcgcgg tgcgcgtgtc
     3961 gaattttgat caggcatacg cgcagctgaa ggcggccggc gtcatgtttt tattcgagcc
     4021 cgtgcaagcc gtcggcggcg ggaagatcgt cagcttccgc gatccggaag ggaacgaact
     4081 ccagatcgtc gagaggtagt gagagctagt gaaggtgcag gattggcaat gaccgtcatc
     4141 ctgaggtact ccgaaggatc taggctgtgg gatacgaagg tctagatcgt tcggagtacc
     4201 tcaggatgac aattta
//
LOCUS       DATIMH010000089         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_310263, whole genome shotgun sequence.
ACCESSION   DATIMH010000089 DATIMH010000000
VERSION     DATIMH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310263"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..656)
                     /locus_tag="VLI90_01580"
     CDS             complement(117..656)
                     /locus_tag="VLI90_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV12921.1"
                     /translation="MVKSNTLLPTRRSRAGVGDDRLLDIIRHHERAILKTCRRWLHDR
                     QDVEDAAQETYLRLFRMSDEVRTNAAGLAYTCAVHVSIDINRRRRTRRQREGAIAAEA
                     SRRHDQPPRQAMMDEHAEHEVHAALQKLEPRLRDLIVQRYFLDRPQVELASEAGVSPS
                     TMSRRLDRAIQRLRTHLRA"
     gene            complement(899..2194)
                     /locus_tag="VLI90_01585"
     CDS             complement(899..2194)
                     /locus_tag="VLI90_01585"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HSV12922.1"
                     /translation="MTAIILSIGDELALGQTVDTNSAWISQQLAAVGCEIFGHATVPD
                     DQAAIEAAIRQAAGRCDFLIISGGIGPTEDDLTRQALGAVMGVELEMDATWLAKLTDF
                     FRSRGREMPEMNKIQAMIPRGAMMIENTAGTAAGIDATYQSGDLKTTCRVFVMPGVPK
                     EMRIMFTRDVLPHIASAGGGAVILSRTLHTFGLGESMVAEKLGDLMNRKRNPSVGTTV
                     AGGIVSLRINARFGSRDEAQKQLDETSDACRAALGDLIFGQDEQTLPQVVGDLLKNGD
                     RRLTVATAESCTGGLLAKMLTDVPGSSAYFRQGWVTYSNEAKYERLGVSMEILNVYGA
                     VSEHVVGAMAKNARRLAKANFALAISGIAGPDGGSTSKPVGTVCIALAAVDPAEKTKS
                     IVQQRTFLFSGDRDMIRDRSAKMALTMLRYHLLQTDLRF"
     gene            complement(2206..2919)
                     /locus_tag="VLI90_01590"
     CDS             complement(2206..2919)
                     /locus_tag="VLI90_01590"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV12923.1"
                     /translation="MSVVQIPSPRKPAPPPPLENGDRLTRAEFERRYHAMPHVKKAEL
                     IGGIVYMPSPVRARQHSTPNSIIITWLGYYVAKTPGLADFGDNATLRLDDQNEPQPDA
                     HLRMAESAGGKSRISEDGYLEGAVEFLSEIAASSVSLDMHAKLQTYEAHGVQEYLVWR
                     VLDEAVDWFALHGGTYAPIAPDQNGIIRSEIFPGLWLDAPALIRRDLPRLLAILDEST
                     ATPQHAAFVERLRTGRGGA"
     gene            complement(2970..>3164)
                     /locus_tag="VLI90_01595"
     CDS             complement(2970..>3164)
                     /locus_tag="VLI90_01595"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HSV12924.1"
                     /translation="VVEHEDFSYGMARKEILCVRCDCHLGHVFDDGPKPTGLRFCVNS
                     ESLVFTPSDKLATLADPAAG"
BASE COUNT          549 a          986 c         1015 g          614 t
ORIGIN      
        1 ccgggagagg gggaaggagc tacatgcgat tgcgctgcct cacgagcacg ctccgaaccc
       61 ggcgttgtcg aggttcttgc gtatcagcgc gtatggcgcg acacgcgcac acgcgctcac
      121 gcgcgcaggt gcgtgcgcag acgctggatc gcgcggtcca atcgtcgcga cattgtcgat
      181 ggcgacaccc ccgcttcgga cgccagctcc acctgcgggc gatccaggaa ataacgctgc
      241 acgatcaagt cgcgcagccg cggctccagc ttctgcaacg ccgcgtgcac ctcgtgctcg
      301 gcgtgctcgt ccatcatcgc ctgccgcggc ggttgatcgt gccggcgcga cgcctcggcg
      361 gcgatggccc cctcgcgctg ccgccgcgtg cgccgccggc ggttgatgtc gatcgacacg
      421 tgcaccgcgc aggtgtacgc cagtcccgcg gcgttggtgc gcacttcgtc cgacatgcgg
      481 aacagccgca ggtatgtttc ctgggccgcg tcttccacgt cctgccggtc gtgcaaccac
      541 cgccggcagg ttttgaggat ggcccgctcg tgatggcgga tgatgtcgag cagccgatca
      601 tcacccacac ccgcgcgcga gcggcgcgtc ggaagcagcg tgttcgattt aaccatctca
      661 gctaccgccc tacgtccatt cagacgagac aggtgaagtt tccttacagg cggcgacgga
      721 tttcgcgcgc cgcattcgaa aagatattgc cggccgggcg ggttttcgga cagcgatttg
      781 aaaagttgcg atgttttaga atcgagggtg actcagagtt caacgacaga tttcacagat
      841 tcagagcgac ccgttcgcaa tgtgcgaaat ccgtgaaatc tgtggttaag ccctgaggtc
      901 aaaagcgaag gtctgtctgc agcaggtggt atcgcaacat cgtcagcgcc attttcgcgc
      961 tgcggtcgcg gatcatgtcg cgatcgccac tgaagaggaa cgtgcgctgc tgcacgatcg
     1021 actttgtctt ctcggccgga tcgactgccg cgagcgcgat gcacacggtg ccgaccggct
     1081 tggacgtcga cccgccgtcg gggccggcga tgccgctgat ggccagcgcg aagttcgcct
     1141 tcgccagccg ccgggcgttc ttggccatcg ctccgacgac gtgctcgctg acggcgccgt
     1201 agacgttgag gatctccatc gacacgccca ggcgttcgta tttcgcttcg tttgaatagg
     1261 tgacccagcc ctgtcggaaa tacgcgctgg aaccggggac gtcggtgagc atcttggcca
     1321 gcaacccgcc ggtgcagctt tcggcggtgg cgacggtgag ccggcggtcg ccgtttttca
     1381 gcagatcgcc cacaacctgc gggagcgttt gctcgtcctg gccgaagatc agatcgccca
     1441 gcgccgcgcg gcaggcgtcg ctggtttcgt cgagctgctt ttgcgcttcg tcgcgcgagc
     1501 cgaagcgcgc gttgatgcgc agcgacacga tcccgccggc gacggtcgtg ccgacggaag
     1561 gatttcgctt gcggttcatc agatcgccga gcttttcagc gaccatgctt tcaccgaggc
     1621 cgaacgtgtg cagggtccgc gaaaggatga ccgccccgcc gccggcgctg gcgatgtgcg
     1681 gcagcacgtc gcgcgtgaac atgatccgca tttccttggg aacgccgggc atgacgaaga
     1741 cgcggcaggt cgtcttcaga tcgccgctct gatacgtcgc atcgattccc gccgcggtgc
     1801 cggcggtgtt ttcgatcatc atcgcgccgc gcgggatcat cgcctggatc ttgttcatct
     1861 ccggcatctc gcggccgcgg gatctgaaga agtccgtcag cttcgcaagc cacgtcgcgt
     1921 ccatctccaa ttcaacaccc atgacggcgc cgagcgcttg gcgcgtgagg tcgtcttcgg
     1981 tcggcccgat gccgccggag atgatgagga aatcgcaccg ccctgccgcc tggcggatgg
     2041 cggcttcgat cgccgcctga tcgtccggca ccgtcgcgtg gccgaagatc tcgcaaccga
     2101 cggcggcgag ctgctggctg atccacgcgg agttggtgtc aacggtctgg ccgagcgcga
     2161 gctcgtcgcc gatggaaaga atgatcgcgg tcatgcgggg agatattagg cgccgccgcg
     2221 tcccgtgcga agacgttcga cgaacgccgc gtgctgtggt gtggcggtgg attcatctag
     2281 aatggcgagt agtcgaggaa ggtcacggcg gatcagcgcc ggcgcatcaa gccatagtcc
     2341 cggaaagatt tcgctgcgga tgatcccgtt ctggtcagga gcaatgggcg catatgtgcc
     2401 gccgtgaagc gcgaaccaat ccaccgcttc atcgagcacg cgccagacaa ggtactcctg
     2461 cacgccatgc gcctcgtacg tttgcagctt cgcgtgcatg tccagactca cgctggatgc
     2521 cgcgatttcc gaaaggaatt cgaccgcccc ttccaaatag ccgtcttcag atatgcgtga
     2581 ctttccgccg gccgattccg ccatacgcag gtgggcgtct ggttgcggtt cgttctgatc
     2641 gtccagtcgg agcgtggcgt tgtctccgaa gtcagcgagg ccgggggtct tggcgacata
     2701 atagcccagc cacgtgatga tgatgctatt gggcgtgctg tgctgccgcg cgcggacggg
     2761 tgaaggcatg tagacgatcc ctccgatgag ctccgccttc ttgacgtgcg gcatggcatg
     2821 ataacgccgt tcaaattcag cgcgcgtcag tcggtcgcca ttctcaagcg gcggcggcgg
     2881 cgcgggcttg cgtggggatg gaatttgaac gaccgacatg ctcggacatt atatcgaatg
     2941 aatcgtccga aatcgccgcg aaaaggccat catcccgccg cggggtcggc tagcgtcgcc
     3001 agcttatcgc tcggcgtgaa caccagcgat tcggaattca cgcagaaccg aagtccggtt
     3061 ggctttggcc catcatcaaa gacgtggcct aaatggcaat cgcagcgaac gcagagaatt
     3121 tcctttctcg ccatgccgta gctgaagtcc tcgtgctcga cgac
//
LOCUS       DATIMH010000090         4612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_20917, whole genome shotgun sequence.
ACCESSION   DATIMH010000090 DATIMH010000000
VERSION     DATIMH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20917"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..2994
                     /locus_tag="VLI90_01600"
     CDS             262..2994
                     /locus_tag="VLI90_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12925.1"
                     /translation="MPQPQAVLTPQAFDPSLSTGTPRVVNDRREAYWATAAVLSAGAL
                     ALVWHFAWFAQDAFIAFRYVANLWSGHGLVWNAGERVQGCTCALWTLLLCLAPKEFTC
                     YWALLLGMITTAITLIAVAVLFARTRRPILGLLVFFCAFFSSKSILEWSTAGMENSLN
                     HALLAILYAAALVGTPGRRRDRVALIAAGLLMFNRLDQLLVVGPVLLYLAVTALHRRE
                     FVAYGRSAIIAFTPLALWLIFATIYYGYPLPNTSYAKLGVPLRAQVTNGLLCLKDFIT
                     CEPWHAAMVFVVCFIAIPLFVRRRGPGAIVLACLALSVALQIVYFIGVGGDYMRGRFL
                     TGDLLLASLAAGYMVAVAPSPMRRGMIWSLATAAVLLPLGIWQTVNEPIFDGVSGVVR
                     IMDYYHPFTEIRTHLKAWRFSELRAEIRRVHMDLDQLAQLNQRAGTDAAMTYGNLGES
                     TYAAIDTPLIDPSGLTDAFIARTRPGAVNRPGHVQRDTPTAYLVSRGCVDQQPGWQRR
                     LAEGDLTLVRDAVAQRDKAKWLHPEAVQVARELAILTRGPIFSRERFALILKYTFGHP
                     IPYDKHDRGPLIYAEEVPPTMLNLSSDLSPALTLLGISPSDASLVGPRQSNCFWLAPT
                     PTAPLAIHVKCAKPQDVYLTFLAREFGYALPVEQDCPVQVSVRGVPQAEFHVRRLGMY
                     LVPLRLPAGVSIITLDVPVAPGSERFFLDSRPLMLYVGNMKLADEPDAAQQIVDLHMR
                     MGEVEGAQPIWYAPADPPSPNDQVGAPPLLQTAGVTSDHAELVAIEPAALAGGYESYR
                     GGPVIWLGPQEAGQIALLVQSDQSIDAKLSGQVEALGAAAPPAGCPLRVLVNGAPLYN
                     GTAAARTTIAAGIHLNPGLNRVTFRVDAEPSPGRFGLDLRPLMLLLSHLQLSPR"
     gene            3237..>4612
                     /locus_tag="VLI90_01605"
     CDS             3237..>4612
                     /locus_tag="VLI90_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSV12926.1"
                     /translation="MAYNFTAIEKKWQQYWLENKTFAALDPAEAGQMPKAYILDMFPY
                     PSGSGLHVGHPEGYTATDIVARYFRMKDVNVLHPMGWDAFGLPAEQYAIKNNVHPADT
                     TRKNIKNFRRQIQMLGLSYDWDREVDTTDPGYYKWTQWIFLQLFNSYFDPIEQKAMPI
                     GHLVNELQNENYVVAPDGTIHINPTQEGLEEIAGEVRVERLWREMTPQEKRDEIDSQR
                     LAYMDEAPVNWCPGLGTVLANEEVIDGKSEVGGFPVERRPMRQWMLRITAYADRLVKD
                     LDMLQWPESLKEMQRNWIGKSVGAEVDFDIAPPEDTPGSVARLREVHEDAEDDLSITV
                     FTTRPDTLYGATYMVLAPEHPLVDRITPPSHRETIEAYRTIIAGKSERDRMAETKEKT
                     GAFTGAFAINPVNDAKIPIYIADYVLMGYGTGAIMAVPAHDERDYEFAKKFDLPIKPV
                     VKPAHGEAP"
BASE COUNT          833 a         1532 c         1423 g          824 t
ORIGIN      
        1 ccggccaacg tcggcgcgaa tatgcgccgc tgctatgaag tcctcattga cgcaaaattc
       61 ttcccgcagg atgagctgaa gctggtcaac gcgtggctgg cggattgcga tgcgatcgat
      121 cgagcggggt gataagtagt cgatgtatct cacacgccgg gttcggaggg tactcccgac
      181 gacccggatt cttcttctga aaacaggcac agcatccgga ccgtcggagt acctccgaac
      241 ccggcgtggt ttaagaatct catgccgcaa ccgcaagccg ttctcacacc gcaggcattc
      301 gatccgtcgc tctccactgg cacgccgcgc gtcgtcaatg atcggcgcga agcgtactgg
      361 gcgacggccg ccgtgctcag cgccggcgcg ctcgcgctcg tctggcactt cgcgtggttc
      421 gcgcaggacg cgttcatcgc cttccgctac gtcgctaacc tgtggagcgg ccacggcctg
      481 gtgtggaacg ccggcgagcg cgtgcagggc tgcacctgtg cgctgtggac gctgctgctg
      541 tgtctcgcgc ccaaggaatt cacctgctac tgggccctgc tgttgggaat gatcacgacg
      601 gccatcacat tgatcgccgt cgccgtgctc ttcgcgcgca cgcgccggcc gatcctgggg
      661 ctgctcgtct tcttctgcgc gttcttctct tccaagagca tcctcgagtg gagcaccgcg
      721 gggatggaga acagcttgaa ccacgcgctg ctggcgatcc tttatgccgc ggcgctcgtg
      781 ggcacgccgg gccggcggcg cgatcgcgtg gcgctgatcg ccgcgggatt gctgatgttc
      841 aatcggctcg accagctgct ggtcgtcggg ccggtgctgc tgtatttggc ggtcaccgcg
      901 ctccatcgcc gcgaatttgt cgcgtacggt cgcagcgcga ttatcgcgtt cacgccgctg
      961 gcgctatggc tcatcttcgc gacgatctac tacggctatc cgctgcccaa caccagctac
     1021 gcgaagctcg gcgtcccgct ccgcgcgcag gtgaccaacg gcctgctttg cctcaaagat
     1081 ttcatcacct gcgaaccctg gcacgcggcg atggtcttcg tcgtctgctt catcgccatc
     1141 cccctctttg tccgtcgccg cgggcctggc gcgattgtgc tcgcgtgcct cgcgctctcg
     1201 gtcgcattac agatcgtcta cttcatcggc gtgggcggcg attatatgcg cggccgcttc
     1261 ctgaccggcg acctgctgct cgcatcgctg gccgccggtt acatggtggc ggtcgcgcca
     1321 agcccgatgc gccgcggcat gatctggtcg ctcgcgacgg ccgccgtgct gctgccgctg
     1381 gggatctggc agacggtgaa cgagccgatc ttcgacggcg tctccggcgt ggtccgcatc
     1441 atggactact accacccgtt caccgaaatc cgcacgcacc tgaaggcgtg gcggttctcg
     1501 gagctccgcg ccgagatccg ccgcgttcac atggacctcg atcagctcgc gcagctgaac
     1561 cagcgcgccg gcacggatgc ggcgatgaca tacggcaacc tgggcgagag cacctacgcc
     1621 gccatcgaca cgccgctcat cgacccatcc ggtctgaccg acgccttcat tgcccgaacc
     1681 cgtcccggcg cggtgaatcg ccccggccac gtgcagcgcg acacgcccac tgcctatctg
     1741 gtctcgcgcg ggtgtgtcga tcaacaaccc ggctggcaac ggcgattggc cgaaggtgac
     1801 ctcacgctgg tgcgcgacgc cgtcgcccag cgcgataaag cgaaatggct tcaccccgaa
     1861 gcggtgcagg tcgcccggga attggccatc ctcacgcgcg gtccaatctt ctcgcgcgag
     1921 cgcttcgcgc tcattctgaa atatacgttc ggccatccaa ttccgtacga caagcacgat
     1981 cgcgggccgc tcatctacgc cgaagaagtg ccaccgacga tgctgaatct gtcttccgat
     2041 ctgtcaccgg cgctcacgct gctcggcatc tcgccgagcg acgcgagcct ggtcggcccg
     2101 cggcaaagta actgcttctg gctcgcaccc acgcccaccg cgccgctggc gatccacgtg
     2161 aagtgcgcca agccgcagga tgtttacctg acattcctgg cgcgcgagtt cggttacgcg
     2221 ctgccggtcg aacaggattg cccggtgcag gtcagcgtgc gcggcgtgcc gcaggcggag
     2281 ttccacgtgc gacgcctggg catgtacctc gtcccgctac gactgcccgc gggcgtctcc
     2341 atcatcacgc tggacgtgcc ggttgcgccg ggctcggagc ggttcttcct cgactcgcgg
     2401 ccgctgatgc tttacgtcgg caacatgaag cttgccgacg aacccgacgc ggcccagcag
     2461 atcgtggacc tgcacatgcg catgggcgag gtcgaaggcg cgcagccgat ctggtacgcc
     2521 ccggctgatc cgccgtcacc gaacgatcaa gtcggcgccc cgccgctgct gcagactgca
     2581 ggcgtcacgt cggatcacgc cgagctggtc gcgattgaac cggccgcgct ggccggtggt
     2641 tacgagtcct accgcggcgg gccggtcatc tggctcggcc cgcaggaagc gggacaaatc
     2701 gcgctgctcg tgcaatcgga tcagtccatc gacgcgaagc tcagcggcca agtcgaagcg
     2761 ctcggcgccg ccgccccacc ggcgggttgc ccactgcgcg tgctcgtcaa cggcgcgcct
     2821 ctgtacaacg gcaccgccgc tgcccgcact acgatcgccg ccggcatcca cctgaacccc
     2881 gggctcaatc gcgtcacctt ccgcgttgac gccgaaccat cccctggccg attcggcctg
     2941 gacctgcgcc cgctgatgct gctgctgtcg cacctgcagc tttcgccgcg ctaattcatt
     3001 gaaacacgaa gggcacgaag atcgcgaaga gaatcagcca tagatgaacg caaatgcaca
     3061 cagataacgt cgagccattc gaggctgatc tgcgtgcatt tgcgtacatc tgttgctgac
     3121 atcccgcttc gtgtccttcg tgccttcgtg gttcagttct ggttttccat caacgcgcac
     3181 gcttcgcacc actccgccga ctgacaacgc cgacgcgccg ccatatattg ccccgcatgg
     3241 cctacaactt caccgcgatt gaaaagaagt ggcagcagta ttggctggag aacaagacgt
     3301 tcgccgccct ggacccggcc gaagccgggc agatgcccaa ggcgtacatc ctggacatgt
     3361 tcccgtatcc cagcgggtcc ggcctgcacg ttgggcatcc cgaaggttac accgccaccg
     3421 atatcgtcgc gcggtatttc cgcatgaagg atgtgaacgt gctgcacccg atgggctggg
     3481 acgccttcgg cctgccggct gaacaatatg ccatcaagaa caatgtccac cccgccgaca
     3541 cgacgcggaa gaacattaaa aactttcgcc ggcagatcca gatgctgggc ctgagttacg
     3601 actgggaccg cgaagtcgat acgaccgatc cgggttatta caagtggacg cagtggattt
     3661 tcctgcagct gttcaacagc tacttcgatc caatcgagca aaaggcgatg ccgatcgggc
     3721 acctggtgaa cgagctgcag aacgaaaatt acgttgtcgc gcccgatggg acgatccaca
     3781 tcaacccgac gcaggaaggg ttggaagaaa tcgccggcga agtgcgcgtc gagcgcctgt
     3841 ggcgcgagat gacgccgcag gaaaagcgcg acgagatcga cagccaacgg ctggcgtaca
     3901 tggatgaggc cccggtcaac tggtgcccgg gcctgggcac ggtgctggcc aatgaggaag
     3961 tcatcgacgg caagagcgag gtcggcggat tccccgtcga acgccggccg atgcggcaat
     4021 ggatgctgcg catcaccgcg tacgccgatc gactggtcaa agacctggac atgctgcagt
     4081 ggccggaatc gctgaaggaa atgcagcgga actggatcgg caagagcgtg ggcgcagaag
     4141 tcgatttcga catcgccccg ccggaagaca caccgggcag cgtggctcgc ctgcgcgaag
     4201 tgcacgagga cgcggaggat gacctgagca tcaccgtctt cacgacccgg cccgacacgt
     4261 tatacggtgc aacgtacatg gtgctggctc cggagcaccc gctggtggat cgcatcacgc
     4321 cgccgagtca tcgcgagacg atcgaggcgt atcgcacgat aatcgccggc aagagcgagc
     4381 gcgatcgcat ggccgagacg aaggagaaga cgggcgcgtt caccggcgcc ttcgcgatca
     4441 acccggtcaa cgatgcgaag attccgatct acatcgccga ttacgtgctg atgggctacg
     4501 gcaccggcgc gatcatggcc gtgccggcgc acgatgaacg agattacgag ttcgcaaaga
     4561 agttcgacct gccgatcaaa ccggtggtga agccggcgca cggtgaagcg cc
//
LOCUS       DATIMH010000091         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3486331, whole genome shotgun sequence.
ACCESSION   DATIMH010000091 DATIMH010000000
VERSION     DATIMH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3486331"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VLI90_01610"
     CDS             <1..787
                     /locus_tag="VLI90_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HSV12927.1"
                     /translation="RGERVSPLSFERPESYRYTGKDEERYMKLNKIDLNASDAPAAPS
                     TQPVGVAKPQAASVRKKTEIVTPKEQLDFLHRTAMDAQVSSDDILRMTRGHNPTGYPP
                     NEFGNGLKTVAAMIKGGLPTRVYYVSLGGFDTHAQERNRHDLLMTQLAAGVSAFWKDL
                     EDQRNADRVMIMTFSEFGRRVEQNASGGTDHGAAAPMFLMGPKITPGLLGRHPSLQDL
                     DAGDLKYNTDFRSVYATILQNWLNTPSKPILGQQFQTLPILRA"
     gene            complement(928..2241)
                     /locus_tag="VLI90_01615"
     CDS             complement(928..2241)
                     /locus_tag="VLI90_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV12928.1"
                     /translation="MSIAATADLILPDATPLDSAAIVAKILRRLIPFLCVLFIVNYLD
                     RTNVAMAKLRMLDDLRITAAAYGFGAGLFFVGYFIFEVPSNLILEKVGARRWIARIMI
                     SWGVISAAMMFTRGTWSFYALRFTLGVAEAGFFPGIVLYLTYWVPAKQRAQTLAAFLT
                     STALSGVIGNPLAGALMKLDGIAGLHGWQWLFLLEGIPPVLLGIAILIFPLLPDRPIH
                     ARWLSDDERRWISEQLARDGVHPHVHHVADLPIAMADARLWLLSAIYFLLIMGLYGFI
                     YWLPTLVHNFTRASDFHVGLLSAIPYLIAAGSMVVIGRLADRRGERQRYVAACAVIGA
                     AGVAIVSQCHSAIPGMLALCLAAIGIFGTLGPFWTIPTRFLRGTAAAGGIAIVNSIGA
                     LAGFVAPSLIGKWFTIGLLVVAGSLVLGSVLVMCVPRSADAGAAD"
     gene            complement(2580..>2797)
                     /locus_tag="VLI90_01620"
     CDS             complement(2580..>2797)
                     /locus_tag="VLI90_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12929.1"
                     /translation="GSFGYAAHRYDLSMKIGELSLFPPVRNAPADAIITAPGTSCRHQ
                     IHDGTAREALHPIEVIDRLIAGPDRAG"
BASE COUNT          586 a          883 c          907 g          421 t
ORIGIN      
        1 gcgcggggag cgcgtgtcgc cgctgtcgtt cgagcggccg gagtcgtacc gctataccgg
       61 caaggacgaa gagcggtaca tgaagctgaa caagatcgac ctgaacgcgt cggatgcgcc
      121 ggcggcgccg tcgacgcagc cggtgggggt tgctaagccg caagcggcaa gcgtgaggaa
      181 gaagacggag atcgtcacac cgaaggagca actcgacttc ctgcaccgaa ctgcgatgga
      241 cgcgcaggtg tcgagcgacg acatcctgcg catgacgcgc ggacacaatc ccaccggata
      301 tccgcccaac gaattcggca acggcctgaa gacggtggcg gcgatgatca agggcgggct
      361 gccgacgcgc gtgtattacg tcagcctcgg cggcttcgat acgcacgcgc aggagcgcaa
      421 ccgacacgac ctgttgatga cccagctcgc cgccggcgtc agcgcgttct ggaaggacct
      481 cgaagaccag cgcaacgctg accgcgtgat gatcatgacc ttcagcgagt tcggccggcg
      541 cgtcgagcag aacgcttcgg gcggcaccga ccacggcgcc gccgccccga tgttcctgat
      601 gggccccaag atcacgccgg gcctgctcgg ccggcacccg tcgctgcaag acttggacgc
      661 cggcgacctg aaatacaaca ccgacttccg cagcgtgtat gccacgattt tgcagaactg
      721 gctgaacacg ccgagcaagc cgatcctggg gcagcagttc caaacgctgc cgatcttgag
      781 agcgtgagga taggcgcgaa agcaaaagca cgacgggttc ggagcgtact cgcgacgaag
      841 cgagattctt cctctagcgc gcgaaagcat ctgggtcgtc ggagtacctc cgaacccggc
      901 gtgtgggcca cagcgcgctg cgcaccgtca atctgctgcg cccgcatccg cgctgcgcgg
      961 cacgcacatc accaaaactg agcccagcac cagcgacccc gcgacaacca gcaatccgat
     1021 cgtgaaccat ttaccgatca ggctaggcgc gacgaagccg gcgagcgcgc cgatcgagtt
     1081 gacaatcgcg atcccgccgg cggcggcggt gcctcttaga aatctcgttg ggatcgtcca
     1141 gaacggcccg agcgtgccga agatgccgat ggcggccagg caaagcgcca gcatgccggg
     1201 gatcgcgctg tggcattgcg agacgatcgc gacgccggcg gcgccgatga ccgcgcacgc
     1261 ggcgacgtag cgctggcgct cgccgcggcg atcggccagc cggccgatga ccaccatgct
     1321 gccggcggcg atcaaatacg gaatcgccga gagcaggcca acgtgaaaat cgctcgcgcg
     1381 ggtgaagttg tgcacgagcg tcggcagcca atagatgaac ccgtacaggc ccatgatcag
     1441 caggaagtaa atcgcgctga gcagccagag tcgcgcatcg gccatcgcga tgggcaggtc
     1501 cgcgacgtga tgcacgtgcg ggtgcacgcc gtcgcgcgcg agttgctcac tgatccatcg
     1561 ccgctcgtca tcggacaacc atcgcgcatg gatcggccga tcgggcagca gcgggaagat
     1621 caggatcgca atgcccagca gcaccggcgg gatgccttcg agcaaaaaga gccactgcca
     1681 gccgtgcagg ccggcgatgc cgtcgagctt catcagcgcg cccgcgagcg ggttgccgat
     1741 cacgcccgac agcgcggtgg acgtgagaaa tgcagcgagc gtttgcgccc gctgtttcgc
     1801 cggcacccag tacgtcaggt acagcacgat gcccggaaag aacccggcct cggcaacgcc
     1861 gagcgtgaag cgcagcgcgt aaaaactcca cgtcccgcgg gtgaacatca tggcggcgga
     1921 gatgacgccc cagctgatca tgatccgcgc gatccaccgc cttgctccca ctttttccag
     1981 gatcaagttg ctcggcacct cgaagatgaa gtagccgacg aagaacagcc ccgcgccaaa
     2041 cccgtaagcc gcggcggtga tgcgcaggtc gtcgagcatc cgcagcttcg ccatcgcaac
     2101 gttggttcgg tcgaggtagt tgacgatgaa cagcacgcac aagaacggga tgagccggcg
     2161 caggatcttt gcaacgatgg cagcagaatc gagcggcgtc gcgtccggca ggatcaggtc
     2221 agcggtggcg gcaattgaca tgtggcggcc gaaagtatcc cgcgtcacga acgctgcggc
     2281 aagataaatc gtcgtcggtt gtcctgatcg ctcaatgact aacgcgcctt tatttccttc
     2341 gcacccgtca cccctccagc gcggcaccta ctcgtgcgag gtgggaggat cttgccgaga
     2401 agtaattgaa cgatccatcc cccccaacac ttttgcagaa gtggcgtggc ttgatctttg
     2461 acacgcgccg cggaacggtg cgcgaaacgg tcgggtcctc agcatctaca cgcggggctt
     2521 cgaacgtcga tgaatgcgtg cgctggaggg tgacgctatg gagagattga agcgccgctc
     2581 tagccagcac ggtcaggccc cgcgataagt cgatcgatca cctcaatcgg atgcagcgct
     2641 tcgcgagcgg tgccatcgtg aatctgatgg cggcaacttg tgcccggtgc ggtgatgatc
     2701 gcatcggccg gcgcgttgcg caccggcggg aagagcgaca gctcgccgat cttcatcgag
     2761 agatcatatc gatgggcggc gtagccaaac gaaccgg
//
LOCUS       DATIMH010000092         5250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4063925, whole genome shotgun sequence.
ACCESSION   DATIMH010000092 DATIMH010000000
VERSION     DATIMH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4063925"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VLI90_01625"
     CDS             complement(<1..372)
                     /locus_tag="VLI90_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12930.1"
                     /translation="MMLVLFFLVGAALAIAPWFLTQLSATAQIVMSGAGLFVLITVAV
                     AAVITKLYRKASANMAFVRTGMGGAQVYQDAGAIVLPVVHQVIPVSLETMRLNVERRG
                     PHALITKDNLRVDLSAEFYIKV"
     gene            complement(369..1205)
                     /locus_tag="VLI90_01630"
     CDS             complement(369..1205)
                     /locus_tag="VLI90_01630"
                     /inference="COORDINATES: protein motif:HMM:NF044375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12931.1"
                     /translation="MTTQWLLGWSNLIFILPFGLGLLYLGIYTLSGWTFGDADADAGV
                     DHEVDASVDADGHLSCEHDADADLSHDVDADADADADTDAHVDADSDADTDADGHDAE
                     SSDSSQSSGSTILAILSWLGVGRVPVSIILMVMLMSWGVIGFAANRTLLDHPVSQAAL
                     LAMAVAFTGSILITKFVTLTVARYLPTNETYARRRHQLLGCVGEALYNIDGTFGMACG
                     RDAVGEQFQVPCRVAEGRAMIAKGTKVQLVGYTASNQMFYVVPADAADRMATQSTERG
                     SL"
     gene            1438..1818
                     /locus_tag="VLI90_01635"
     CDS             1438..1818
                     /locus_tag="VLI90_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12932.1"
                     /translation="MRFRPGHLALLIFQAIWLNVILPGHTRGVVTTPGTECSACESSS
                     APQRTTTCCGSSSDSKRQQTPNRRAQHCAICAFAARLTLPPVIDLTPAPLGFLERLAL
                     APPARVESVRSLPTYDGRAPPAFA"
     gene            1879..2865
                     /locus_tag="VLI90_01640"
     CDS             1879..2865
                     /locus_tag="VLI90_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HSV12933.1"
                     /translation="MQLSLFARRRIVTAAAAFCVLLYPLVARANHGPGASGGGSATLS
                     GETLKPGSFEFDLREDYTQFEHFGRNEAISRAIEGGGGFDALDHGFLTTVEGAVGVFE
                     DFQLGASIGYFVGRDFISADRADDGSFDVSTAQPSGLTDLVLTGKYRVLKGQPGNLSI
                     VGGVIFPTGRNDVALANGESLSPTDQPGTGRWGFPIGLAYSRFLTSHLTFDASVLYTS
                     RLQRADFRVGNRFDVGLAFAYRLTGSIKHFPQFSIFGEVNSVYLYRDRSDAQGYDPNS
                     GGETIYLTPGGRVRFGPHTALTLAPSFPIYQHLYGNQGKVDFKLAFTFSVSL"
     gene            2884..3192
                     /locus_tag="VLI90_01645"
     CDS             2884..3192
                     /locus_tag="VLI90_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HSV12934.1"
                     /translation="MSNTNRFAVACVFASIAALCGCADESQTVARGPTTAPIAAMTSP
                     RAQCLVCKYNADLACLDVDVDQKTPSYVYQGKTYYFCSQECHDEFAKHPQRYAPLASK
                     "
     gene            3200..5011
                     /locus_tag="VLI90_01650"
     CDS             3200..5011
                     /locus_tag="VLI90_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HSV12935.1"
                     /translation="MTTIESLERRVLLAAAPIAWQRLADAPTIRAEGTKAVVNDKLYT
                     VGGFVSPQLAVTKELDVYDPLANSWTRLADMPAPETHGGSAVFGTDIWVAGFFFNNGI
                     TASNLVYKYDTVNNTWTQEPSLPGARGAVGMAVLDHTLHVWGGLTGQTKSAADHWALD
                     LDNIAAGWHTLAPLPKPLDHIGGVALNGRIWSIGGFVDKNEFSGNLTVVYRYDPTTDQ
                     WTTDVAPLPIALGHIGPDTIVTGDKIVIAGGQINSDRDEETTVVLQYNSAADQWTQLT
                     ALPEPRKSASLGFADGELILSDGNQESSPFLSKKTWVAKYDPATGGAPEPTPTPTPTP
                     TPTPTPTPTPTPTPTPTPTPTPTPTPLPTDPNGPDLTGVLRVRLRPNAVAGQPGLAGV
                     IVTNAGPAAAIGNVNIALSISPDGTADSAVAIATISGGVLRLGARRARGFFIPFTLPA
                     DLAGGTYTFFTTIDTANAFTEVDETNNIATGPAAAVAAQVHDTSATGLFAPAVLRAGR
                     PGGLFFFLQNAGNAIENGVISLTVTATDAAGTPLRILNLTPRVILVPHQRRFVLLRFV
                     VPKELSGGATLTVTAQPLTFADADASDDVTSRAITAV"
BASE COUNT          980 a         1784 c         1551 g          935 t
ORIGIN      
        1 caccttgatg taaaactccg ccgacaaatc gacgcgcaga ttatccttcg tgatcagcgc
       61 gtgcgggccg cggcgttcca cgttcagccg catcgtttcc agcgacactg gaatcacctg
      121 atgaacgacc ggcagcacga ttgcaccggc gtcctgatac acctgcgccc cgcccatgcc
      181 ggtgcggacg aacgccatgt tcgccgacgc cttgcggtac agcttcgtga tgaccgcggc
      241 gacggccacc gtgatcagca cgaacagccc ggcaccggac atgacgatct gcgcggtggc
      301 ggataactgt gtcaggaacc acggcgcgat cgcgagcgcg gcgccgacga ggaagaaaag
      361 taccagcatc atagcgagcc cctttccgtt gattgcgtag ccatgcgatc cgccgcatca
      421 gcggggacga catagaacat ttgattgctt gccgtgtaac cgaccagctg caccttcgtc
      481 cccttggcga tcatcgcacg gccttcggcc acgcggcagg gaacctggaa ttgctccccc
      541 accgcatcgc gcccgcaggc catgccgaag gtgccgtcga tgttatagag cgcttcgccg
      601 acgcacccga gcagctgatg ccgccggcgg gcgtatgttt cgttcgtcgg cagatatcgc
      661 gcgacagtca gcgtcacgaa tttcgtgatc aagatgctgc cggtgaaggc taccgccatc
      721 gcgagcagcg ctgcttgcga gacaggatga tcgagcagcg ttcgattggc ggcgaaaccg
      781 atcacgcccc agctcattag catcaccatc aggatgatgc tgactggcac ccggccaaca
      841 ccgagccagc tgaggatggc gaggatcgtg gagccgctcg attgcgatga gtcggaagat
      901 tccgcgtcgt gcccatctgc gtccgtgtcg gcatccgaat cagcatcgac atgcgcatcg
      961 gtgtccgcgt ccgcatcggc gtccgcgtcc acatcatgcg acagatccgc atccgcatcg
     1021 tgttcacatg acaggtgccc atcagcgtcc accgacgcgt cgacctcatg atccacaccc
     1081 gcgtcggcgt cggcgtcacc gaaggtccag ccgctgagcg tgtagatgcc aaggtagagc
     1141 aggcccagcc cgaagggcag gatgaagatc aggttcgacc accccaacag ccattgcgtt
     1201 gtcatagttc acgctcctgc cgggccactg aaagggccgc aaggcggatc acgaacaacg
     1261 aggaatagca tggtgctttc attaccgccg cacaagggtt ttggcggtat aaagcgaagg
     1321 catgtgtgta acgatcggga gcaagttcga ttggctcgcc cgacaccccg ggagaggggg
     1381 taaggaccat cgccgcttga cgcgcgttcg atatccctgc catcattccc cgcgttcatg
     1441 cgtttccgcc ccgggcatct cgcgctgctg atcttccagg cgatctggct gaacgtcatc
     1501 ctccccgggc acacccgcgg cgtggtgacg acgccgggga ccgaatgcag cgcgtgcgaa
     1561 tcttccagcg cacctcagcg aacaaccacc tgctgcggtt catcatccga ctctaagcga
     1621 caacagacgc cgaatcgccg cgcgcagcac tgcgcgatct gtgcgttcgc cgcgcgactg
     1681 accttgccgc cagtaatcga tttgaccccg gcgccgctgg ggtttctcga gcggttagcg
     1741 ctcgcgccgc cagcgcgggt tgaatcggtt cgatctctac cgacctacga cggccgcgcc
     1801 ccgcccgcct ttgcctgatc tgttcaaccg ttcgatcaga cgcacctgca atcgaaatca
     1861 ggcgaaggaa ccccctacat gcaactcagc ttgttcgcgc gccggcgcat cgtcaccgca
     1921 gccgccgcgt tttgcgttct cctgtatccc ctcgtcgccc gcgccaacca cggccccggc
     1981 gccagcggcg gcggtagcgc caccctctcc ggcgagacac tcaagcccgg cagcttcgaa
     2041 ttcgacctcc gcgaggacta cacgcagttc gaacatttcg gccgcaacga agcgatcagc
     2101 cgcgccatcg aaggcggcgg cggatttgac gcgctcgacc acggcttcct caccaccgtc
     2161 gagggagccg tcggcgtgtt cgaagacttc cagctcggcg ccagcatcgg ttacttcgtc
     2221 ggccgcgatt ttatttccgc cgatcgcgcc gacgacggca gcttcgatgt ctccaccgcc
     2281 caacccagcg gcctgaccga tctcgtgctc accggcaaat atcgcgtcct gaagggccag
     2341 cccggcaacc tctcgattgt cggcggcgtc atctttccca ccggacgaaa cgatgtggcg
     2401 ctggccaacg gtgaatccct ctcccccacc gaccaacccg gcaccgggcg atggggtttt
     2461 ccaatcggct tggcttattc gcgattcctc accagccatc tcaccttcga tgccagcgtc
     2521 ctctacacct ctcgcctgca gcgcgccgac ttccgcgtcg gcaatcgctt cgacgtcggc
     2581 ctggccttcg cctatcgcct caccggatcg atcaagcact tcccgcaatt ttccatcttc
     2641 ggtgaagtga acagcgtcta cctctatcgc gaccgcagcg atgcccaagg ctacgatccc
     2701 aacagcggcg gcgagacgat ctacctcacg cccggcggtc gcgtgcgatt tggcccgcac
     2761 acggctctca cactcgcgcc gtctttcccc atttatcagc atctttacgg caatcagggg
     2821 aaggtcgact tcaaattggc cttcaccttt tccgtctcac tctgatacgt tcacaggagc
     2881 agcatgagta acacaaaccg ctttgcagtc gcttgcgtct tcgcgagcat cgccgcgctg
     2941 tgcggatgcg ccgacgagag ccaaaccgtc gcgcgcggcc ccactaccgc gcccatcgca
     3001 gcgatgacca gccctcgcgc ccagtgcctc gtttgcaaat ataatgcgga cttggcctgc
     3061 ctggacgtgg atgtcgatca gaagacgccc agctacgttt accagggcaa gacctattat
     3121 ttctgcagcc aggaatgcca cgacgagttc gcgaagcatc cccagcgcta cgcgccgctg
     3181 gcgagcaagt agaacaacga tgacgacgat tgaatcgctg gaacgacgcg tcctgctggc
     3241 cgccgcgccc atcgcctggc agcgcctcgc cgatgcgccg acgattcgcg ctgaaggcac
     3301 caaggccgtc gtcaatgaca agctttacac cgtcggtggc ttcgtgagcc cgcaattggc
     3361 ggtcactaaa gaactggacg tgtacgaccc tctcgccaac agctggacgc gattggcgga
     3421 catgcccgcg ccggaaacgc acggcgggtc cgccgtcttc ggcaccgaca tctgggtcgc
     3481 cggtttcttc tttaacaatg gtattactgc cagtaatctg gtctacaaat acgacacggt
     3541 gaacaatacc tggacgcagg aaccgtcgct gccgggcgcg cggggggccg tcggcatggc
     3601 ggtgctcgat cacacgctgc acgtctgggg tgggctgacc gggcaaacca agtcggcggc
     3661 cgatcattgg gcgctggacc tggataacat cgccgcgggc tggcacacgc tggctccgtt
     3721 gcccaagccg ctggatcaca tcggcggcgt ggcgctcaac ggtcgcatct ggtcgatcgg
     3781 cgggttcgtc gataagaacg aattcagcgg caacctcacg gtcgtctatc gctacgatcc
     3841 gacgaccgac caatggacaa ccgacgtcgc cccgctgccg atcgcgctgg ggcacatcgg
     3901 tcccgacacg atcgtgaccg gcgacaagat cgtcatcgcc ggtgggcaaa tcaattctga
     3961 tcgcgatgaa gagacgacgg tggtgctgca atacaactcg gcggccgatc agtggacgca
     4021 gttgacggcg ctgcctgagc cgcgcaagag cgcaagcctc ggcttcgccg atggcgaatt
     4081 gatcttgagc gacgggaatc aggagtcgtc gccgttcctg agcaagaaga cgtgggtagc
     4141 taagtacgac cccgcgaccg gtggtgcgcc ggagccgacg ccgacgccga cgcccactcc
     4201 gactccgact cctactccta cgcccacgcc tacaccgacc ccgacgccca cgcccacgcc
     4261 cacgcccaca ccgacgccgc tgccgacgga tccgaacggg cccgatctca ccggcgtgct
     4321 tcgcgttcgc ctgcggccga acgcggtcgc gggtcaaccg gggctcgccg gcgtgatcgt
     4381 caccaacgcc ggccccgcgg cggccattgg caacgtcaat atcgcgctgt cgatttcacc
     4441 cgatggcaca gccgacagcg cggtggcaat cgcgacgatc agcggcggcg tgctgcgcct
     4501 cggcgcccgc cgcgcgcgtg ggttcttcat cccgttcacg ctgcccgccg acctggccgg
     4561 cggcacctac acgttcttca ccacgatcga caccgccaac gcgttcaccg aagtggatga
     4621 gacgaacaac atcgccaccg gccccgcggc ggcggtggcg gcgcaggtgc acgacacgtc
     4681 ggcgaccggg ctgttcgcgc cggcggtcct tcgcgccggc cggccgggtg ggttgttctt
     4741 cttcctgcag aacgcgggga acgcaatcga gaacggcgtg atctcgctga cggtcaccgc
     4801 gaccgatgcc gctggaacgc cgctgaggat tttgaacctg acaccgcggg tgatcctggt
     4861 cccgcatcag cggcggtttg tgttgctgcg gtttgtggtg ccgaaggaat tgtcgggcgg
     4921 cgcgacgctg acggtgacag cgcaaccgct gacgttcgcg gatgctgatg cgagtgatga
     4981 cgtgacgtcg cgcgcgatta ctgcggtatg aacaaaaaag cccgcggcta tgccgtggga
     5041 cgcatttcga acacgaagtc ccacggcata gccgtgggct gttccttttg ctattcgagt
     5101 tacttgtcca cgatgacgac ttgatccggt gagtcggcga ccaactccgg tccatcgtga
     5161 tacagccgga ctttgccaga gacgcggatc gtcttgccga cgagggcggc ggagacgtcg
     5221 gtgccgaagc gttgctccat cctctcgaac
//
LOCUS       DATIMH010000093         1923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3487582, whole genome shotgun sequence.
ACCESSION   DATIMH010000093 DATIMH010000000
VERSION     DATIMH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487582"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..>1923)
                     /locus_tag="VLI90_01655"
     CDS             complement(103..>1923)
                     /locus_tag="VLI90_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV12936.1"
                     /translation="PVAFISGITGQFYRQFALTIAFSTLLSAFNSLTLSPALAALLLR
                     PHSAKPDWFQRMLDIGFGWFFRLFNRGLELTTHGYVRVLRPIVRLSVIALFVYAGLLV
                     LAYFGFKTVPTSFIPSQDQGYLICAVQLPDAAALDRTDAVMRHVASIANQVAGVRATI
                     ALPGFSILTGANQSNAGTMFVSLQPFDERAKDPSKSGRAIIGKISGQFAQVQEGLALV
                     FPPPPVRGIGTAGGFKMQVEDRSGLATPQELQATVERLMAEARKDTRLTSLISTFRAS
                     VPQLYADIDRTKLKQQNVAVTDVFQALQVYLGGLYVNDFNYLGRTFRVVAQADAPFRA
                     HVADVAQLKTRNSKGQMVPLGSVMELKQVTGPDRINRYNLYTSAEINGSAAPGISSGQ
                     ALSIMEDLAHQYLPTGYSFEWTELAYQEELAGNTALYIFPLCVLFVFLTHSAEYESFA
                     LSAAIILIVPMCLLCGIAGVWMRGMDNNIFTQIGFIVLAGMSVKNAVLIVEFAHQQQS
                     RGKNAFEAAVQASRLRLRPILMTSFAFIFGVLPLTIAIGAGAEMRQALGTVVFFGMIG
                     VTFFGVFLTPVFYYVIRILTRHTAAEVAAEEPAPEAQAPT"
BASE COUNT          371 a          615 c          610 g          327 t
ORIGIN      
        1 cccacacgca ccaccaccgc cgcgggcgac agccagcggg cggctttgga gcaactgccc
       61 ggtcgattgt cgaaaagacc cgctggatgt cgcccgcggc tatcaagttg gtgcttgcgc
      121 ttcgggcgcg ggttcttccg ccgccacttc tgctgcggtg tgtcgcgtga gaattcggat
      181 gacgtagtaa aataccggcg tcaaaaacac gccgaagaac gtgacgccga tcatgccgaa
      241 gaagacgacg gtgcccagcg cctgtcgcat ttcagcgccg gcgccgatgg cgattgtcag
      301 cggcagcacg ccgaagatga acgcgaagct cgtcatcagg atcggccgca ggcgcaggcg
      361 cgaggcttgg accgcggcct cgaacgcatt cttgccgcgc gattgttgct ggtgcgcgaa
      421 ctcgacgatc agcacggcgt tcttcacgct catgccggcc agcacgatga agccgatctg
      481 cgtgaagatg ttgttgtcca tcccgcgcat ccacacgccg gcgatgccgc acaagaggca
      541 catggggacg atcaggatga tggcggcgga cagcgcgaag ctttcgtact ccgccgagtg
      601 cgtgaggaac acgaacagca cgcacagcgg aaagatgtac agcgcggtat tgcccgccag
      661 ctcttcctga tacgccagct ccgtccattc gaaactgtag cccgtcggca gatattgatg
      721 cgccaggtcc tccatgattg aaagcgcctg tccgctgctg atgccgggcg cagcgctgcc
      781 gttgatctcg gccgaggtgt acaagttgta tcgattgatg cgatcgggac cggtgacctg
      841 cttcagttcc atcacggaac ccagcggcac catttgcccc ttgctgttgc gcgtcttgag
      901 ctgcgccaca tccgccacgt gcgcgcggaa cggcgcatcg gcctgggcga cgacgcggaa
      961 cgtgcggccc aggtagttga agtcgttgac gtacagccca cccagataaa cctgcagcgc
     1021 ctggaacacg tcggtcacgg cgacgttctg ctgcttcagc ttcgtgcgat cgatgtcggc
     1081 atacagctgc ggcacgctcg cgcggaaggt ggagatgagg ctcgtcagcc gcgtgtcctt
     1141 gcgcgcctcc gccatcaagc gttccacggt cgcctgcagt tcctgcggcg tagccagccc
     1201 gctgcggtct tccacctgca tcttgaaacc accggccgtg ccgatgccgc gcaccggcgg
     1261 cggggggaag accagcgcca gcccctcctg cacctgggca aactgcccgc tgattttccc
     1321 gatgatcgcg cgacccgatt tgctggggtc cttcgcgcgc tcgtcgaacg gctgaagcga
     1381 cacgaacatg gttccggcgt tggattggtt ggcgccggtg agaattgaaa atcccggcag
     1441 cgcgatcgtc gcgcgcacgc ccgcgacttg gttcgcgatg ctcgccacgt gccgcatcac
     1501 cgcgtcggtg cgatcgagcg cggcggcatc cggcaattgc acggcgcaga tcaggtagcc
     1561 ctgatcctgc gaaggaatga acgatgtcgg cacggttttg aaaccgaaat acgcgaggac
     1621 caacaggccc gcatagacga acagcgcgat gacgctcagg cgaacgatcg gccgaagcac
     1681 gcggacgtaa ccgtgcgtcg tcagctcgag cccgcggttg aaaagtcgga agaaccagcc
     1741 gaagccgatg tccagcatcc gctggaacca atccggcttt gccgagtgtg gccgcagcag
     1801 caacgccgcc agcgccgggc tgagcgtcag cgaattaaac gccgacagca gcgtggagaa
     1861 cgcgatcgtc agcgcgaact gccggtagaa ctgccccgtg attccgctga tgaacgcgac
     1921 cgg
//
LOCUS       DATIMH010000094         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_22819, whole genome shotgun sequence.
ACCESSION   DATIMH010000094 DATIMH010000000
VERSION     DATIMH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22819"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VLI90_01660"
     CDS             complement(<1..1061)
                     /locus_tag="VLI90_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12937.1"
                     /translation="MAAHTPGSDRAHLHAVLDTRGAAVIVALLLVIGTIAFYTSNVLP
                     TELAVLTSDGAMAACWTAGACCCGAMVLRTLSPSPGTPGEGWSEGDSEFRKPLDDRNH
                     PHPGPLPEYRARGPERALHIATAGGLGLGIFSLVGLGLGLLGWLNQITALALPFLSIL
                     AFAIQLFRHRPELHNDNITRWWTAPAGRAWFWLMPAIALATASVVASDLPGDLWKPMD
                     PHPYDVVSYHLQVPREWFEAGRIVPLRHNVFSYFPVNVEMQYLLLMHATGGPWRAMYA
                     CQFMSVAYTALMLLAIAGASEFGVIGAAFAAVVPWTIMLGGVAYEESALMLYTALALV
                     WALRACCVLFLATPGSERTR"
     gene            complement(1070..>2338)
                     /gene="hemA"
                     /locus_tag="VLI90_01665"
     CDS             complement(1070..>2338)
                     /gene="hemA"
                     /locus_tag="VLI90_01665"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSV12938.1"
                     /translation="LNVRERLAFNADDRAAALAAFRQRFANCEAVLLSTCNRVELYVA
                     REVHGHPRAEEMAEFLAELRQVPTSSFRPHLYEKSDHAVVDHLFNVASSLDSMVLGET
                     QILGQVREAYDAAREVSAAGPMLHPLFQRAIAVGKQVMHETPLAEGRLSVASVAVDYA
                     RRIFDHFDDKLVLNIGAGKMATLVLRHFAELKPKQLIIANRDPAKAAALAQRFGGQTA
                     AFEQLDEQLASVDIVITSTGSARPIITAQRFAAVHKRRRFRPIFLIDIALPRDVEAAV
                     GELENVYLYNLDDLQQVVSQTQAGRSGAIDAARRIVAAAVEDHARALRVREMGPVIDQ
                     LYKRYHQVAQEELARTLNKLPNVTEAERQHLEELARRIVNKLLHDPVHALRRADETHA
                     PAEQYLHAMERLFKLTLPETPKGDDAAQAD"
BASE COUNT          398 a          808 c          756 g          376 t
ORIGIN      
        1 cgagtacgct ccgaacccgg cgtggccaag aataagacgc agcacgcgcg cagcgcccac
       61 acaagcgcca gcgccgtata cagcatcaac gccgattcct catacgccac cccgccgagc
      121 atgatcgtcc acggcaccac ggcggcgaag gcggcgccga tcacgccgaa ctcgctcgcc
      181 cccgcgatcg ccagcagcat cagcgccgtg tacgcgacgc tcatgaactg gcacgcgtac
      241 atcgcccgcc acggtccccc ggtggcgtgc atcagcagca ggtactgcat ctccacgttg
      301 accggaaaat acgaaaacac gttgtgccgc agcggcacga ttcgccccgc ctcgaaccat
      361 tcccgtggca cctgcaggtg atagctgacg acgtcgtacg gatgcgggtc catcggcttc
      421 cacagatcgc cgggcaaatc cgaagcgacg acggacgcgg tggccaatgc aatggccggc
      481 atcaaccaaa accacgcgcg cccggcgggc gctgtccacc atctcgtgat gttgtcgttg
      541 tgcagttccg ggcggtgacg gaagagttgg atcgcgaacg cgaggatgct gaggaacggc
      601 agcgccagcg cggtgatctg gttcagccaa ccgagcagac cgaggccgag cccaacgagg
      661 ctgaagatgc ccaggcctag tccaccggcg gtggcgatgt gcagcgcgcg ctccggtccc
      721 ctcgcccggt actccgggag agggccaggg tgagggtgat ttcgatcgtc gagcggcttt
      781 cgaaattctg aatcaccctc actccaaccc tctcccgggg taccgggaga gggggaaaga
      841 gttcgcagca ccatcgcccc gcagcagcac gcgccggcgg tccagcacgc ggccatcgcg
      901 ccgtcgctcg tcagcaccgc cagctccgtc ggcagcacat tgctcgtgta aaacgcgatg
      961 gtgccgatga ccagcagcag cgcgacgatc acggccgccc cgcgcgtgtc cagcaccgcg
     1021 tgcaggtgcg cccgatcgga tccaggggtg tgagcagcca tcacgtcgat caatccgctt
     1081 gtgccgcgtc atcgcctttg ggcgtttcgg gcagcgtcag cttgaacagc cgctccatcg
     1141 cgtgcaggta ttgttcggcc ggcgcgtgtg tttcgtccgc gcggcgcagc gcgtgcaccg
     1201 ggtcgtgcag cagcttgttg acgatccgcc gggccagctc ttccaggtgc tgccgctcgg
     1261 cctcggtcac gttcggcagc ttgttcagcg ttcgcgcgag ctcctcctgc gccacctggt
     1321 gatatcgctt gtacagctga tcgatgaccg gccccatctc gcgcacgcgg agcgcgcggg
     1381 cgtgatcttc gacggcggcc gcgacgatcc gccgggcggc atcgatcgcg ccgctgcggc
     1441 cggcctgcgt ctgcgacacc acctgctgca ggtcgtccag gttgtacaga tagacgtttt
     1501 ccagttcgcc cacggccgct tccacgtcgc gcggcagcgc gatgtcgatc agaaagatcg
     1561 gccggaaccg ccgccgcttg tgcacggcgg cgaaccgctg ggcggtgatg atcggccgcg
     1621 ctgagccggt cgaggtgatg acgatgtcca cgctggccag ctgttcgtcc agctgctcga
     1681 acgccgcggt ttgaccgccg aagcgctggg cgagcgccgc cgccttcgcg gggtcgcggt
     1741 tggcaatgat cagctgcttc ggcttcagct ctgcgaagtg ccggagcacc agcgttgcca
     1801 tcttgccggc gccgatgttc agcacgagct tgtcgtcgaa gtgatcgaag atccgccggg
     1861 cgtaatcgac ggcgacgctg gcgacgctga gccgcccctc ggccagcggt gtttcgtgca
     1921 tcacctgctt gcccacggca atcgcccgct gaaacagcgg gtgcagcatc ggccccgccg
     1981 ccgacacctc gcgggcggca tcgtacgcct cgcgcacctg tccaaggatc tgcgtctcgc
     2041 cgagcaccat ggaatcgagc gacgacgcga cgttgaacag gtgatcgacg accgcatggt
     2101 cggacttctc gtacagatgc gggcgaaatg acgacgtcgg cacctggcgc agctcagcga
     2161 gaaactccgc catctcctcc gcgcgcgggt gcccgtgcac ttcgcgcgcg acgtacagtt
     2221 ccacgcggtt gcaggtggac agcagcaccg cttcgcagtt ggcgaagcgc tggcgaaatg
     2281 ccgccagcgc tgcggcgcga tcgtccgcgt tgaacgccag ccgctcgcgg acgttcaa
//
LOCUS       DATIMH010000095         3581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_312466, whole genome shotgun sequence.
ACCESSION   DATIMH010000095 DATIMH010000000
VERSION     DATIMH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312466"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VLI90_01670"
     CDS             complement(<1..653)
                     /locus_tag="VLI90_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV12939.1"
                     /translation="MPLVASVQNLSKIYRKPGTNIEVAALRNINLDFEQGEFTAIMGA
                     SGSGKSTLMNILGCLDQPTTGSYILGDIDISQLDDDTLSEIRSQRIGFIFQNFNLIQQ
                     LTVLENLEVPMFYLGISPAERRRRAMELAERVGLAERADHRPMQLSGGQQQRVCIARA
                     MVNEPLILLADEPTGALDSKTGQQILELFDELVAQGKTIMLVTHDPSVGHRANRVIHL
                     "
     gene            850..2145
                     /locus_tag="VLI90_01675"
     CDS             850..2145
                     /locus_tag="VLI90_01675"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HSV12940.1"
                     /translation="MVLPLILILLATLTAGVLAYGTDAGWAQFNHGIDFIIWSRRLQW
                     PLIVTALLLCVALLGLVIAGKRRAWWLIALLPILALFLHRFVTGPARTVEVMEDPQFV
                     DAEQATFLQDRDYVVGVRFADGAYAFPYSVIYGAPAIVVSDRERRMMLMWSPFANRAL
                     AYNVGREVRGRDLEIVSTPANALLLYNSRLGQFINGITGLTTRRERPLGFREPLQTLK
                     LPWATWRALSPETRVMSPLDLRWRTQPNKPVLPKFPMPRSRPELADARPVCVVATTQP
                     IAVPAEAVKQTPLNLTAGKTPVLLFRDSATSALRAFDRHVDEDLSPRFSVASDPKHPD
                     VALVDSDTNTEWSAAGVAEALPKDAKPKRLAPIPVEDELYWGVMKFWYPELTLVGPDA
                     LAAASTAGEQVVAPPTNGAKEKAPQPPKRRKRSVATPAR"
     gene            2370..3476
                     /locus_tag="VLI90_01680"
     CDS             2370..3476
                     /locus_tag="VLI90_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSV12941.1"
                     /translation="MQYGVIMAGGSGTRLWPISRGDKPKQLLRVVRGKSLLQVSYDRL
                     RGILPANQIYVCTASAHADLVLQDLPELSRENIIGEPVGRDTANAVGYAAAVLRKRDP
                     DAVCAVVTADHVIEPIDTFQAAIKTAFNVTAEYPNALVTFGIVPTYGHTGLGYVHRGE
                     QLKLRDGAAGAYKVLAFKEKPDKPTADRYVESGRYYWNSGMFVWRADTVLNEIATLLP
                     ESYKGLSAIADAWDTPRREEVLNAIYPTLKKISVDFAIMEPAAQGKGKAQVTVVEMPV
                     QWLDVGSWTTLAETLKTDEHNNAVDGETMLFVDSDDNIVVSTDAKHLVATIGVSDMII
                     VHTPDATLVCPKNESQRVKDLVAKLKDRFGERFQ"
BASE COUNT          639 a         1087 c         1201 g          654 t
ORIGIN      
        1 aggtgaatga cccggttggc ccgatggccg acgctagggt cgtgcgtcac cagcatgatg
       61 gtctttccct gggcgaccag ctcgtcgaac agttccagga tctgctgccc ggttttgctg
      121 tccagcgcgc cggtcggttc gtcggccagc aggatgagcg gttcgttcac catcgcgcgg
      181 gcgatgcaca cgcgctgctg ctgtccaccc gagagttgca tcggccggtg atccgcgcgc
      241 tcggccagcc cgacgcgctc ggccaattcc atcgcccgcc ggcggcgctc ggccggcgag
      301 atgcccaggt aaaacatcgg cacctccagg ttctccagca cggtcagctg ttggatcaag
      361 ttgaaattct gaaagatgaa cccgatgcgc tggctgcgga tttcgctgag cgtgtcatcg
      421 tccagttggc tgatgtcgat gtcgccgagg atgtagctgc cggtcgtcgg ctggtccagg
      481 caaccgagga tgttcatgag cgtgcttttg cccgacccgc tggcgcccat gatggcggtg
      541 aattcgccct gctcgaagtc gaggttgatg ttgcgaagcg ccgccacctc gatgttggtg
      601 ccgggcttgc ggtagatctt gctgaggttc tgtacggatg cgacgagggg catagtcgat
      661 caattgtagc gggcaatcga ctccccgcgt agcggtagcc ccgaaggggc acgtgttttt
      721 gcgttcgaac gtaaaacaca tatcccttcg gggctaccgc cacgcatgag acgacgcggg
      781 cgcgacgtat catcgcgcga cgcatcgcgc gactcgaaat tgcgcatcct cgcggttacc
      841 ttcgccgccg tggtccttcc gctcatcctg attttgctcg ccacgctcac cgcgggggtg
      901 ctggcttacg gcacggacgc cggttgggcg cagttcaacc acgggatcga cttcatcatc
      961 tggtcgcgcc gcctgcagtg gccgctgatc gtgacggcgc tcttgctgtg cgtggcgttg
     1021 ctcggcctgg tgatcgccgg caaacgccgg gcgtggtggt tgatcgcgct gctgccgatc
     1081 ctggccctgt tcctgcaccg ttttgtcacc gggccggcgc gaacggttga agtgatggaa
     1141 gacccgcagt tcgttgacgc cgagcaggcg acgtttttgc aggatcgcga ctacgtcgtc
     1201 ggcgttcgct tcgccgatgg cgcgtacgca tttccgtatt cggtgatcta cggcgcgccc
     1261 gcaatcgtcg taagcgaccg cgagcggcgg atgatgctga tgtggtcgcc gttcgccaac
     1321 cgcgcgctgg cgtacaacgt tggccgcgag gtgcgcgggc gcgatttgga gatcgtctcg
     1381 acgccggcga acgcgttgct gctgtacaac tcgcggctcg ggcagttcat caatggcatt
     1441 accggcctga caacccgccg cgagcgcccg ctcggctttc gcgagccgct tcaaacgttg
     1501 aagctgccat gggcgacatg gcgcgcgttg agccccgaga cgcgggtgat gtcgccgctg
     1561 gacctgcgct ggcggacgca gccgaacaaa ccggtgctgc cgaagttccc gatgccgcgg
     1621 tcgcggccgg agctggcgga tgcccggccg gtctgtgtgg tcgcgacgac gcaaccgatc
     1681 gcggtgccag ccgaagcggt gaagcaaacg ccgctgaacc tgacggcggg gaagacgccg
     1741 gtgctgttgt ttcgcgattc ggcgacgagc gcgctgcgcg cgtttgatcg tcacgtggat
     1801 gaagatctgt cgccgcgatt ttcggtggca tcggatccga agcatccgga cgtcgcgctg
     1861 gtggattccg atacgaatac ggaatggtcg gcggccggcg tcgcggaggc gctgccgaaa
     1921 gatgccaagc caaagcggct ggcgccgatc cccgtcgagg acgagctgta ctggggcgtg
     1981 atgaagttct ggtatccaga gctgacgctg gttggtcccg atgcgctagc cgcggcatca
     2041 acagcggggg agcaggtcgt cgcgccacca acgaacggtg cgaaagagaa ggccccgcag
     2101 ccgccaaaac gccggaaacg ttccgtggcg acgccggcgc gataagcgac ctcgtagtgc
     2161 aatttccccg cgtagcggta gccccgcagg aagacgtgtt ttcccgctgg aacgaaaaac
     2221 gcgtgcccct gcggggctac cgctacgcat gggagaccac gcgccgcgga gcgctccggc
     2281 gaaaaagata tgcggactaa cggttgatcg gccgatgttt ctgatataac gggatggggc
     2341 gcgagtgtcg agacggagcg gattgagtta tgcaatatgg cgtaatcatg gcaggggggt
     2401 cgggcactcg gctttggccc atttcccgcg gcgacaagcc caagcaactg ctgcgcgtcg
     2461 ttcgtggaaa gagcctgctg caagtcagct acgaccgcct gcgcggcatc ctcccggcca
     2521 accagattta cgtctgcacc gcgtcggcgc acgccgacct tgtattgcag gacctgccgg
     2581 agctgtcccg cgaaaacatc attggtgaac ccgtcggccg cgatacggcc aacgccgtgg
     2641 gttacgccgc ggctgtgttg cgcaagcgcg atcccgacgc ggtctgcgcg gtcgtgaccg
     2701 ccgaccacgt catcgaaccg atcgacacgt tccaagccgc catcaagacc gcgttcaacg
     2761 tcacggcgga atatcccaac gcgctggtca cgttcggcat cgtgccgaca tacggccaca
     2821 cgggcctcgg ttacgttcac cgtggcgaac agctgaagct gcgcgacggc gcagccggcg
     2881 cgtacaaggt gctggcgttc aaggaaaaac ccgacaagcc gacggcggat cgctatgtcg
     2941 aatccggccg gtactactgg aacagcggca tgttcgtttg gcgcgccgac acggtgttga
     3001 acgagatcgc gacgttgttg ccggaatcgt acaagggcct gagcgccatc gccgatgcgt
     3061 gggacacccc gcgtcgcgag gaggtgctga acgcgatcta tccgacgctg aagaagatca
     3121 gcgtggactt cgccatcatg gaaccggccg cccagggtaa gggcaaggcg caggtgacgg
     3181 tggtggagat gcccgtgcag tggctggacg tcggctcgtg gaccacgctg gccgagacgc
     3241 tgaagaccga cgagcacaac aacgcggtgg acggcgagac gatgctgttc gtagacagcg
     3301 acgacaacat cgtcgtcagc acggacgcga agcacctggt cgcgacgatc ggcgtgagcg
     3361 acatgatcat cgtacacacg cccgatgcaa cgctggtgtg tccgaagaac gaaagccagc
     3421 gcgtgaagga tttagttgcg aagttgaagg atcggtttgg ggagcggttt cagtaacgac
     3481 gcgctgatgt cgcttgcaca tgccccgacg ccggtgtctg agcgtactcg cgaagacccg
     3541 ggtatcttcc gcgccagcac ccgggtcttc ggagtacctc a
//
LOCUS       DATIMH010000096         4417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4644278, whole genome shotgun sequence.
ACCESSION   DATIMH010000096 DATIMH010000000
VERSION     DATIMH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4644278"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VLI90_01685"
     CDS             <1..376
                     /locus_tag="VLI90_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HSV12942.1"
                     /translation="IRMLVDGDITDAIPVKYASRADYEALLQQGVEIAEYQPTMMHAK
                     VAIVDDGLSIVGSANFDNRSLEMNDELNVAIFDRELAGRLRADFDRDLTRSKTLRLDR
                     WRARPLTEKTRDWLFSYFGEVF"
     gene            complement(416..784)
                     /locus_tag="VLI90_01690"
     CDS             complement(416..784)
                     /locus_tag="VLI90_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HSV12943.1"
                     /translation="MSGHISPKSTYYAIFGALMVLTGVTIAVAFVHLGSLNFPVALAI
                     AITKATLVLLFFMHVKYSSRLVKMVVGMAFFFLLIMFGLTMTDYLSRGWFTGPYGTTS
                     AGTQVTITRTPAPVQSESGH"
     gene            complement(800..1504)
                     /locus_tag="VLI90_01695"
     CDS             complement(800..1504)
                     /locus_tag="VLI90_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HSV12944.1"
                     /translation="MPDAVALHAEPHGHGPAHAHHPAQKHHFQTLEQQAEAATLGMWL
                     FLVTEVMFFGGLFTAYLLYRVWYPAAWSEGSQELNIVLGGTNTAVLIASSLTMALAVR
                     AAQTGFRKSSITFLLLTMALGLTFLIIKFFEYREKYRLSHIPGPNFHFEGPHAGQVEI
                     FFSLYFALTGLHAIHMLIGFGIMSVILWMSVKNRFSPQWYTPVEMAGLYWHFVDIVWI
                     FLFPLLYLVDRAHNVA"
     gene            complement(1497..3107)
                     /locus_tag="VLI90_01700"
     CDS             complement(1497..3107)
                     /locus_tag="VLI90_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HSV12945.1"
                     /translation="MTITESPVNYLNVRHGVASWLLTQDHKRIGLMYMVVVTIAFIIG
                     AFFAAGIRIELATPEGDFVSSDTYNKLFTMHGVAMVFFVLIPAVPAILGNFVLPLQLG
                     AKDVAFPKLNLLSWYVFVFGLIFTLIAMVTGGVDTGWTFYPPYSTEGSTSNVLLAGIG
                     VFITGFSSILTGLNFMVTIHRMRAPGLTWFRLPLFIWAVYATSLVQVLGTPVIAITVA
                     MVAVERAFGLGFFDPRIGGDPVLFQHLFWFYSHPAVYIMILPPMGVISEVIACFSRKN
                     VFGYSFVAFSSLAIAVFGFLVWAHHLFVAGISPYSAMVFSFLTFAVAIPSAVKTFNWT
                     ATLYRGAIWWKSPLMWTFGFLGLFLVGGLTGLFVASLAIDVHVTDTYFVVAHFHYIMV
                     GGAIMGYFAGLHYWWPKMSGKMFNDTLAKVAALLVFIGFNMTFFPQFILGYLGMPRRY
                     HMYPPEFQILNLMSTGGAAILGLGYLLPMIYFTWSWFYGEKAPANPWLATGLEWKTQS
                     PPLPENFPETPIVTEGAYEYSKREIKVA"
     gene            complement(3115..4122)
                     /gene="coxB"
                     /locus_tag="VLI90_01705"
     CDS             complement(3115..4122)
                     /gene="coxB"
                     /locus_tag="VLI90_01705"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HSV12946.1"
                     /translation="MLSGISLLPTQASPYASEVDNLYFFIVAVTAFFAILVVVLVLYF
                     AIKYRDDTGLKVGAPITGSIPLEIGWSLVPFVVAMAIFVWATAVYFHAVRPPNQTMEI
                     YSTGKRWMWRFQHVDGQREINQLHVPIGRPIKVTFTSEDVLHDLYIPAFRVKADAIPG
                     RYSSIWFLPTKTGEYHLFCAEYCGTRHSGMIGTVYVMEADEYQAWLSGGAGTGSMSAR
                     GEQLFQQLACSSCHLTDGTGRGPSLAGVFGSQVKLQAGQAVHADESYVRESILTPQMR
                     IVQGYQPIMPTFQGLVTEEQLLSLIEYVKSLSANESVGQQASSTPRAEAPAAATSGER
                     K"
     gene            complement(4128..>4417)
                     /locus_tag="VLI90_01710"
     CDS             complement(4128..>4417)
                     /locus_tag="VLI90_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12947.1"
                     /translation="GVLVVTPDGRLARYFYGVEYSPKELRFALVESGQGHIGSAIDEL
                     LLYCYHYNPEQGRYGATVMNLVRGAAVLTLAAMAGFFISQRRRRSDPPMAH"
BASE COUNT          976 a         1292 c         1379 g          770 t
ORIGIN      
        1 cattcggatg ctcgtcgacg gcgatatcac ggatgccatt ccggtgaagt acgccagccg
       61 cgcagattac gaggcgctcc tccagcaggg cgtcgagatc gcggagtatc agccgacgat
      121 gatgcacgca aaggtcgcca tcgtcgacga cgggctgagc atcgtcggct cggcaaactt
      181 cgacaaccga tcgttggaaa tgaacgatga gttgaacgtg gcgatcttcg atcgggagtt
      241 ggccggacgt cttcgcgcgg atttcgatcg cgatctgacc cgatcgaaga ccctgcgtct
      301 ggatcgatgg cgtgcgcgtc cgctcacgga aaaaacgcgc gactggctat tcagttactt
      361 cggagaggtg ttttaggatt cgtgattctt gattcgtgat tcttgattgc gggtatcagt
      421 ggccgctctc tgactgcaca ggcgccgggg tcctcgtgat cgtcacctgg gtgccggcgc
      481 tggtcgttcc gtaggggccc gtgaaccagc cgcgcgacag atagtcggtc atcgtcaaac
      541 cgaacatgat cagcagaaag aagaaggcca tgccgacgac catcttgacc aggcggctgc
      601 tgtacttcac gtgcatgaag aacaggagga cgagcgtggc cttggtgatg gcgattgcca
      661 gggccaccgg gaagttgagc gatccgaggt gcacgaacgc caccgcgatc gtgacgcccg
      721 tgagcaccat gagcgcgccg aaaatcgcgt agtacgtgct ctttggagaa atgtgtcctg
      781 acatggctcg tgaaccgcgt taggcgacgt tgtgcgctct gtcgacgaga tagagcagcg
      841 gaaacagaaa aatccagacg atgtcgacga agtgccaata gagaccggcc atttccaccg
      901 gtgtgtacca ctgcggcgag aaacggttct tcaccgacat ccagaggatg acggacatga
      961 tgccgaagcc aatcagcata tggatcgcgt gcaggccggt gagcgcaaag tagagcgaga
     1021 agaagatctc aacctgtccc gcgtgcggtc cttcgaagtg aaagttcggc cctggaatgt
     1081 ggctaagccg gtacttctct cggtactcga agaacttgat gatcaggaag gtcaggccga
     1141 gcgccatcgt gagcagcaga aaggtgatcg agctcttgcg gaaccctgtc tgtgccgcgc
     1201 ggacggccag cgccatcgtc aagctgctcg cgatcaggac ggcggtgttc gtgccaccga
     1261 gaacgatgtt cagctcctga ctgccttcgg accatgctgc cggataccag acgcgataca
     1321 gcaggtaggc cgtgaacagg ccgccgaaga acatgacttc cgtgacgagg aagagccaca
     1381 tgccgagcgt tgccgcttcg gcctgctgct ccagcgtctg gaagtgatgc ttctgcgctg
     1441 gatgatgggc gtgtgccggg ccgtgcccgt gaggctcggc gtgtagggcg acggcgtcag
     1501 gcaactttga tctcccgctt cgagtactcg taggcgcctt cggtgacgat cggcgtctct
     1561 gggaaattct ccggaagcgg cggtgactgc gtcttccact cgagtccggt ggcgagccat
     1621 ggattcgccg gcgccttctc accgtagaac caggaccacg tgaaatagat catcggcagc
     1681 agataaccga gaccgaggat cgcggcgccg ccggtcgaca tcaaattgag gatctggaac
     1741 tccggcggat acatgtgata gcgccggggc atcccgaggt atccgaggat gaactgcggg
     1801 aagaacgtca tgttgaagcc gatgaacacg agcagcgccg ccaccttggc cagggtgtcg
     1861 ttgaacatct tgccgctcat cttcggccac cagtagtgaa gaccggcgaa gtagcccatg
     1921 atcgcgccgc cgaccatgat gtagtggaag tgcgcgacca cgaaatacgt gtcggtgacg
     1981 tgaacgtcga tcgcgagcga cgcgacgaac agaccggtaa ggccgccgac caggaagagg
     2041 ccgaggaacc cgaacgtcca catcagcggt gatttccacc agatcgcgcc gcgatagagc
     2101 gtcgccgtcc agttgaaggt cttcaccgct gaaggaatag caacagcgaa agtgaggaac
     2161 gagaacacca tcgcggagta cggggaaatg cctgcgacga agagatggtg cgcccacacc
     2221 aggaaaccga acaccgcgat cgcaaggctc gagaaggcca cgaacgaata gccgaagacg
     2281 ttcttcctcg agaagcaggc gatcacctcg ctgatcacgc ccatcggcgg caggatcatg
     2341 atgtagaccg cgggatgcga atagaaccag aacagatgct ggaagagtac ggggtcgcca
     2401 ccgattcgcg gatcgaagaa gccgagaccg aacgcccgtt ccaccgcgac catcgcgacc
     2461 gtgatcgcaa tgaccggtgt gccgaggacc tgaacgaggc tggtcgcata caccgcccag
     2521 atgaagagcg gcaggcgaaa ccaggtgagc cccggtgcgc gcatccggtg aatcgtcacc
     2581 atgaagttca ggccggtcag gatcgacgag aagccggtga tgaatacgcc tattccggcc
     2641 agtagcacgt tcgacgtcga cccttcggtg ctgtatggcg gatagaaggt ccaccccgta
     2701 tcgacgccac cggtcaccat cgcgatcagc gtgaagatca ggccgaagac gaacacgtac
     2761 cagctcagaa gattcagctt cggaaaagcg acgtccttcg ccccgagctg cagcggcagc
     2821 acgaagttgc cgagaatggc gggcacggcc ggaatcagga cgaagaacac catcgccacg
     2881 ccgtgcatcg tgaacagctt gttgtacgtg tcgctggaca cgaaatcgcc ttccggcgtc
     2941 gcgagctcga tccggatgcc ggccgcgaag aacgcgccga tgatgaaggc gatcgtgacg
     3001 acgaccatgt acatcaagcc gatgcgcttg tggtcttgcg tgagcagcca cgaggcgacg
     3061 ccgtgccgga cgttgaggta attgaccggt gactcggtga ttgtcattgg ggttctactt
     3121 tcgttcaccc gaagtcgccg ccgctggtgc ttccgcacgt ggcgtcgagc tcgcctgctg
     3181 tccaaccgat tcattggcgg acagcgactt cacgtattca atcaggctga ggagctgttc
     3241 ttcggtgacg agcccctgga aggtcggcat gatcggctgg tagccctgca cgattcgcat
     3301 ctgcggcgtc aggatcgact cgcgcacata gctttcgtcg gcgtggaccg cctggccggc
     3361 ttgcagcttc acctgtgatc cgaacacccc ggccagagag ggcccgcgac cggtgccatc
     3421 agtgagatga catgacgagc aggcgagctg ctggaacaac tgctcgccgc gcgccgacat
     3481 cgatccagtt ccggcgccgc cgctcaacca ggcctggtac tcatccgcct ccataacgta
     3541 gacggtgccg atcatgccgg aatgcctagt gccacagtac tccgcgcaaa acagatgata
     3601 ttcgccggtc ttggtgggca agaaccagat cgagctgtag cgacccggaa tcgcatccgc
     3661 tttgacgcga aacgccggaa tgtacagatc gtgcagcacg tcttccgagg tgaacgtcac
     3721 tttgatcgga cgcccgatcg gcacgtgcag ctgattgatt tccctctggc cgtcgacgtg
     3781 ctggaagcgc cacatccacc gcttgccggt cgaataaatc tccatcgtct gattgggcgg
     3841 ccgcaccgcg tggaaataca cggcggtcgc ccagacgaag atcgccatcg ccacgacgaa
     3901 cggcaccaac gaccagccaa tctccagcgg aatcgatccg gtgatcggcg cgcccacctt
     3961 cagccccgtg tcgtcgcgat acttgatcgc gaagtacagc acgaggacga cgacgaggat
     4021 cgcgaagaac gccgtcaccg cgacgatgaa gaagtacagg ttgtccacct cggatgcgta
     4081 aggggacgcc tgcgtcggaa ggagagaaat tccagacagc atagaagtta gtgggccatc
     4141 ggcggatccg agcgccggcg gcgctgcgag atgaagaagc cggccatcgc cgccagcgtc
     4201 aataccgcgg cgccgcgaac caggttcatc actgtggcgc cgtatcgtcc ctgctccgga
     4261 ttgtagtggt agcagtagag caacagctcg tcgatcgccg agccgatgtg accctggccc
     4321 gattcgacca gcgcgaagcg cagctccttc ggcgagtact cgactccgta aaaataccgc
     4381 gccagtcggc catccggtgt gacgaccagg acgccgc
//
LOCUS       DATIMH010000097         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_889334, whole genome shotgun sequence.
ACCESSION   DATIMH010000097 DATIMH010000000
VERSION     DATIMH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889334"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1877
                     /locus_tag="VLI90_01715"
     CDS             240..1877
                     /locus_tag="VLI90_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12948.1"
                     /translation="MIRHQGHSLKFTAAIRPPAAVAPKRVTRGRRSRSSMKRLALLLP
                     APLLLYALPASATVSLTMVNGAATSATVAPGNSFAFTVKLTSSSTAAVDQVTAVDYFL
                     TSQLASAPVSNIFTIASRNTQSDSSAFTDVYALDANVVTAGAGPGLNPTNGTDLGGSE
                     SNTNVPVSNATVIVADYSVSVNAAAPSGVYKVLTFSTPGTGYSGAGPTFTDHSFTSQG
                     SFTANVVRAGETLWTGGTSTWGTAGNWLKAAVPVATDDAALGAGGPYTVTVSAASVAK
                     NLNVYGNATLNFTGNQQLTLGGSLNLTSNNNPSLTLTGSGSVVMSSGTAVVGSAGGPA
                     KLILSGGGLVTSATQINNGGQVILSLAASAASPNVFNAGALTFNGTGTLDVGNNELLT
                     TTPQGTIRTNLISNAIFTSHTGTNTALGYGDGGGGLTEVRYTLKGDATLNGTVDVGDL
                     GALATNYGATTGATWQQGDSNYDGQVNVADLGALATNYGTSLAAGSIASGTPVAQPTD
                     LAATSTAVPEPASWLPALVGMAGVYTRRSRQRTLNQL"
     gene            complement(2141..2737)
                     /locus_tag="VLI90_01720"
     CDS             complement(2141..2737)
                     /locus_tag="VLI90_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV12949.1"
                     /translation="MNCHELQLLRNVDSSRRGGIVEQLPLGLINLNAKGAVIWSTATA
                     RQMLLAHFDDAKDSVHGLPNDVRLWWRSVSTAKANGTANHLRSVRPGFELRIRLCPLK
                     DGSVVLLLQENPDVRTTRTLNDFALTPREQQVMQSLLQGRSVSEVATQLLISPRTVQK
                     HLERIFPKLGVNNLTAACVKLISSHGSSTHNLEPAGHR"
BASE COUNT          544 a          917 c          884 g          482 t
ORIGIN      
        1 tgcggtcagc agtaccgggc gaggggacca gagcgcgcgg ttgttacatg cgtttgccct
       61 gacacagcac ccagtaccct cattttcccg tttttgcttg tatcgcccca ccacggcggg
      121 ttaggcttgc gggccgttca agattctcgc tcaaggggcg actcgcggcc aagcgagtat
      181 cctgctccag gcgtcgcgcg atctcccggc tggcagcagt aacatccgga gggttcatca
      241 tgatcaggca tcagggtcat tcgttgaaat tcaccgccgc catccgcccg cccgctgctg
      301 tcgcgcccaa gcgcgtgacg cgcggccgac gcagccgatc gtcgatgaag cgcctcgcgc
      361 tgcttcttcc ggcgccgctg ctgctgtacg cgcttcctgc gtcggccacg gtcagcctga
      421 cgatggtgaa cggcgccgcc acgtcggcga cggttgcgcc cggcaactcg tttgccttca
      481 ctgtcaagtt aaccagcagc tccaccgccg ctgtggatca ggtcacggcg gtcgattatt
      541 tcctgacgtc acaactggcg tcggcgccgg tcagcaacat cttcaccatc gccagccgaa
      601 acacgcagtc cgacagcagc gcgttcacgg atgtctacgc cttggacgcg aacgtcgtga
      661 cggccggcgc cggcccggga ttgaacccga ccaacggcac ggatcttggc ggctcggaat
      721 cgaacaccaa cgtcccggtt tcgaacgcga cggtgatcgt ggcggactac agtgtgtccg
      781 tgaacgccgc cgccccgtcg ggggtttaca aggtgctgac cttcagcacg ccaggcactg
      841 gatattccgg cgccggaccc acgttcaccg accactcgtt cacctcacag ggatcgttca
      901 ccgccaacgt ggtgcgcgcg ggtgagactc tctggacggg ggggacatct acgtggggca
      961 ccgccggcaa ttggttgaag gcggccgtgc cggtggcgac ggacgacgcg gcactcggcg
     1021 ctgggggacc gtacaccgtc accgtcagcg cggcgtcggt tgccaagaac ctgaacgtct
     1081 acggcaacgc gacgctgaac ttcacgggca accaacaact cacgctgggc ggatcgctga
     1141 acctgacgag caacaacaat cccagcctca cgctcaccgg cagcggcagc gtcgtgatga
     1201 gcagcggcac cgccgtcgtc ggctcggccg gcggtccggc aaagctgatc ctgagcggcg
     1261 gcggcctcgt taccagcgcc acgcagatca acaacggcgg gcaagtaatc ctgtccctcg
     1321 ccgccagcgc ggcgtcgccg aacgtcttca acgccggcgc gctcaccttc aacggcaccg
     1381 gcacactgga cgtgggcaac aacgaactgc tcaccaccac gccgcaaggc acgatccgca
     1441 cgaacctgat cagcaacgcc atcttcacgt cgcacaccgg cacgaacacg gcgctcggtt
     1501 acggcgacgg cggcggtgga ctgaccgagg tgcgctacac gctcaagggc gacgcgaccc
     1561 tgaacggcac cgttgacgtc ggcgatctgg gcgcgctcgc caccaattac ggcgccacca
     1621 ccggcgccac ctggcaacag ggcgacagca actatgatgg ccaggtcaac gtcgccgatc
     1681 tgggcgcgct ggccacgaac tacggcacca gcctggccgc gggttcgatc gcgagcggaa
     1741 cgccggtggc acagccaacg gacctcgccg caacatcaac cgccgtgccc gaaccggcat
     1801 catggctgcc agcgctggtg ggcatggcag gcgtgtacac gcgccgctcg cggcagcgca
     1861 cacttaatca gttgtagcgt tccgacaaat accggccgaa tccagttaag tccccgagcg
     1921 tttgccatgg tgacgtttga aaccatgggt agaaaccgcg gccgaaatcg ggatagagag
     1981 attctagctg gcaaggaacc acggatcgcg ggaggtgcgc gggtgcataa gccaacgccg
     2041 ctctttgact ggcgcgagct tcagcgtgca cacgtcaccg agaagcaact gccccctcca
     2101 ccgccgtgcg atccttctcg cctgccggca tgaaatatct ctatcgatgt cccgctggct
     2161 ccagattgtg cgtgctgctg ccgtgcgaac tgatcaactt gacgcaggca gctgtgaggt
     2221 tgttgacacc gagcttggga aaaatgcgct ccagatgttt ttgaacggtg cgcgggctga
     2281 tgagcagttg cgtggcgacc tctgagacgg accggccctg caagagagat tgcatgacct
     2341 gctgttcgcg aggggtgagg gcgaagtcgt taagcgtccg cgtcgttcgc acgtccggat
     2401 tttcctgcag cagcaggacg acgctgccgt ctttcagcgg acagaggcgg atgcggagtt
     2461 cgaatcccgg ccggaccgaa cgcaggtggt tcgcagttcc atttgccttg gcggtagaaa
     2521 cggatcgcca ccagaggcgc acgtcattgg gcaaaccgtg cacggaatcc ttggcgtcgt
     2581 caaaatgcgc cagaagcatc tgccgggccg tggcggttga ccagatcacc gcgccctttg
     2641 cattaagatt gatgagccca aggggcagct gttcgacgat gccgccgcgg cggctgctgt
     2701 cgacattgcg gagcagttga agctcgtggc aattcaaata ggcctgaagc aggtgcgggc
     2761 gaagtagatt aagcagctcg cgctcgagat acttccaaaa gttgttgatg gccgcgacag
     2821 ctcaagc
//
LOCUS       DATIMH010000098         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4932412, whole genome shotgun sequence.
ACCESSION   DATIMH010000098 DATIMH010000000
VERSION     DATIMH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4932412"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1131)
                     /locus_tag="VLI90_01725"
     CDS             complement(79..1131)
                     /locus_tag="VLI90_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HSV12950.1"
                     /translation="MGFRQRETSLTFALCASMTVHALTLSAAAWWYVRQTPPPRLAAL
                     PRMEEAAAPIIVPPKEAPPPPPPPAAQPQAPKPAPLPPPDFSKATKVPLRDDSGEAVG
                     HGTANRSTPGDRPMEAAAGLEQANLTRDTNNDVKSKVDDAPMPAQDGSPLGESALAEA
                     TPSFGVPKAADVQPAPAPRQPMIAIADPTHAGAPAAPAPTPPTEAADLGSPTGAKDEA
                     PNPILAPKWAPKEVLGHSSISSDTESPPFAKANSAHFHNGRMDARQGRRVKTTRIDFD
                     IAATTAYLAMGSASVTLGVNVDAAGYVQSVVILRSSGSDAIDLPAERAVYNWWFEPTK
                     DKSGRSLPDQWVVTID"
     gene            complement(1198..1812)
                     /locus_tag="VLI90_01730"
     CDS             complement(1198..1812)
                     /locus_tag="VLI90_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HSV12951.1"
                     /translation="MSSVIHSVTVYCSSSTAIPRAYFDAAAELGAAIASRKWTLVYGG
                     NFVGLMAAVAQAARDRGGRVVGVTPQLMVDKGLSDQKADELIVTNTMRDRKAIMEERG
                     GAFVALPGGLGTFEEVFEIIVGKQLKYHDKAIVLLNVNRYFDPLLQMIEHGIEQRFIK
                     SKARDLYFVAPTVAAAMDYLRHYVPPAPSADKWFTPAGPPSGAE"
     gene            complement(1956..2342)
                     /gene="apaG"
                     /locus_tag="VLI90_01735"
     CDS             complement(1956..2342)
                     /gene="apaG"
                     /locus_tag="VLI90_01735"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HSV12952.1"
                     /translation="MPTPTLSDVTTDGIRVGASAFYLPDQSDPTQQRYVFGYTIVISN
                     EGDAPAQLISRHWVIIDANGHREEVNGPGVVGKTPRLEPGQAFKYQSFCPLKTPWGTM
                     EGEYQTRRDNGETFDARIERFYLRMS"
BASE COUNT          470 a          881 c          871 g          515 t
ORIGIN      
        1 cacgccgact agcttgcctt cgccttggat ggagtagaag agttcggaga tgcgcatggc
       61 gtttgaaata aatgcctatc aatcgatcgt gacgacccac tgatccggca acgatcgccc
      121 ggatttatcc tttgtgggtt cgaaccacca attgtagacc gcgcgctccg cgggcaggtc
      181 gatcgcgtcg ctaccgctgg agcgcaggat gaccacgctc tgcacgtatc ccgccgcgtc
      241 cacgttcacg cccagcgtca cacttgcgct gcccattgcc aggtacgccg tggtcgcggc
      301 gatgtcgaaa tcgatccgcg tcgtcttcac ccgccggcct tggcgggcgt ccattcggcc
      361 gttgtgaaag tgcgcactgt ttgccttggc gaacggcggt gattcggtgt cgctgctgat
      421 cgatgaatgc ccgagcactt ccttcggcgc ccacttcggc gccaatatcg gattcggcgc
      481 ttcatccttc gcgccggtcg gcgagcccag gtcggccgct tcagtcggcg gcgttggcgc
      541 cggcgcggcg ggggcgccgg cgtgcgttgg atcggcgatg gcgatcatcg gctggcgcgg
      601 cgcgggcgcc ggttgaacgt cggccgcttt cggcacgccg aacgacggcg ttgcctccgc
      661 cagcgcgctc tcgcccagcg ggctcccatc ctgcgcgggc atcggggcat cgtctacctt
      721 cgacttcaca tcgttgttcg tatcgcgcgt caggttcgcc tgctccagtc ccgcggccgc
      781 ttccatcggg cgatcgcccg gcgtcgaacg attcgccgtg ccgtgaccga cggcctcgcc
      841 gctgtcgtcg cgcagcggca ccttggtcgc cttcgaaaaa tccggcgggg gcagtggcgc
      901 aggcttgggc gcttgcggtt gcgcggccgg cgggggcggg ggcggcggag cttccttcgg
      961 tgggacgatg atcggcgccg cggcctcctc catgcgcggt agcgccgcca accgcggagg
     1021 tggcgtttgc ctcacgtacc accacgcggc cgccgagagc gtcagcgcgt gcaccgtcat
     1081 cgacgcgcac agcgcaaacg tcagcgatgt ttcgcgttga cgaaatccca tcgggatact
     1141 cagggtccac caccgtgcgc cgcgtgcgtg atcgcaacat tatatggcgg ggcaggctta
     1201 ctcggcaccc gagggtgggc cagcgggtgt aaaccattta tcggccgacg gggcgggcgg
     1261 gacgtaatgc cgcaggtaat ccatggcggc cgcgactgtt ggggcgacaa aatacaaatc
     1321 gcgagccttg gatttgatga accgctgctc gatgccgtgc tctatcatct gcagcagcgg
     1381 gtcgaagtag cgattgacgt tcagcagcac gatggccttg tcgtgatact tcagctgctt
     1441 accgacgatg atctcgaaga cttcttcaaa ggtgccgagc ccgcccggca gcgcgacgaa
     1501 cgcgccgccg cgctcttcca tgatcgcctt gcgatcgcgc atcgtgttgg tgacgatcag
     1561 ctcatcggcc ttctggtcgg acaagccctt gtccaccatc aactgcggcg tcacgccgac
     1621 cacgcgtcca ccacgatcgc gcgccgcctg cgcgacggcg gccatcaggc cgacgaagtt
     1681 cccgccgtat accagcgtcc atttccgcga tgcgatggca gcgcccaact ccgcggcggc
     1741 gtcaaagtag gcgcggggaa tcgcggtgct ggaggaacag taaacggtga cggaatgaat
     1801 cacggaagac atgccggcag tataggcggc gcgggtcatt cagaaaggga agtcggatgc
     1861 gcggcgagac atgcatctcg ccgcttgcgg cttcgcaatc caagcggcgc agtttgcaaa
     1921 tccgcaagca gcacatttgc gcagcttggc gccgatcaac tcatccgaag ataaaaccgc
     1981 tcgatccgcg catcgaacgt ctcgccgtta tcccgccgcg tctggtactc gccttccatc
     2041 gtgccccacg gcgtcttcag cgggcagaag ctctggtact tgaacgcctg ccccggttcc
     2101 aaccgcggcg tcttgccgac gacaccggga ccgttcactt cttcgcgatg accgttggcg
     2161 tcgatgatca cccagtgccg gctgatcagt tgggcggggg cgtcgccttc attcgagatc
     2221 acaatcgtgt agccgaacac gtaccgctgc tgggtcggat cggattgatc cggcagataa
     2281 aacgcactgg cgccgacgcg aatgccgtcg gtggtcacat cagacaaagt cggggtgggc
     2341 atacgtcctc acacacaaac atctcgatcc acggtgtcga aactcagcgg gaaagatatc
     2401 tcacattcgc cgagcgtgcc acggaatgcg ccggagcatt ttctttgcag cctcgtcagg
     2461 atttcgctgg caggttcagc gggcgcctcg gtacgatggg cagcgcagat ggcttgtacc
     2521 gtgtggaggg gtcccgtcgg tcccaagcag aggaggcgac aatggccaag ctgttgatcg
     2581 tcgacgacga gattgccgaa gtccgcccgc tgatggccct gctccgccag gccggtcacg
     2641 atgtctgttg cgccaccacg ccgggtgaag cgctgatcga gctgcagcac aacacgccgg
     2701 acctggtgtt gctcgacctg aacatgcctt acctcaa
//
LOCUS       DATIMH010000099         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_313178, whole genome shotgun sequence.
ACCESSION   DATIMH010000099 DATIMH010000000
VERSION     DATIMH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313178"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="VLI90_01740"
     CDS             complement(<1..1489)
                     /locus_tag="VLI90_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HSV12953.1"
                     /translation="MGRLLVIPLVLLALLVAALAWSGGGGARSRPDFTFINRGEIGTL
                     DPNRMSWMQDIRVGYMIWEGLYALDPVTLDPVPGAAQSIDINPDKTVYTFHLRSDGRW
                     SNGDAVVAGDFAFAWRRMLEEPGDYSYLLNYIKGAKAYSQNFVDNKPADFASVGIEAL
                     DPHTLRVTLNHPVTFFPDLCAFAPFFPLNERSMRPFQKKDEPTGHVTYDKAFTRHLVG
                     NGPYNLVDWQFKRGVRFEANPNYWDHANVKSKVIYMVSADDPLAAFLKYDSGAVDWQA
                     EITGDIAAELLRAHRPDLHVFPSFGTYFYSVNCQPKLPDGRDNPLADARVRQALTMSI
                     DKQAIVETITRLGEKPADKYIPPGIFNHFESAPGLGYDIARARQLLNDAGYPGGRGFP
                     QLSLLYNNEANHGDIAQNVRRQWIEHLGIDFKPEPVEIKTFRERLHKKDYAIARASWF
                     GDYNDVSTFTDKYLSFSDNNDSDWKNQKYDDLCAAAAVQADAGKRL"
     gene            complement(1606..>2949)
                     /locus_tag="VLI90_01745"
     CDS             complement(1606..>2949)
                     /locus_tag="VLI90_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12954.1"
                     /translation="LAGARRVHVRGRARMMANEQRRRCKHPPENRLMNRRVSVRHRVG
                     IPASVCVARAGLIALSGLWLAGGVALAQPAAPSLPSIGGGAGATAAAGPTLPTTIKNA
                     PNAAAFIPQIQQFIQGQLQQLGGEDAAAQKAARERLINECTPGSTQSFFDVYAQVLSQ
                     EIQNLLKQPAGKSLRARLAMAVIVERVATVGKTTKVEPAVIDLINDPLEPVAIKAMQA
                     AKPVLELVYQTPAQIAADKLVMAIKPAVTKHAKPAFPNDEAPGYLIADAYDALMPEAS
                     PTLKADQVTAAMPFVLDPILDLLDFRIQLYTASIPPQPSAESRLPTFFSRSYGGANPK
                     QQLRIVQSLVNLIEVAGQQAQNATKAQLQELIPVLKYAAGTLIVTSPNQPAVQTVLAP
                     IGQLNVNTPGPQVAQKTKGVFAAMQLQFKDLKAPPPVTLAAPTTAAAPTTAAPTH"
BASE COUNT          516 a          906 c          953 g          574 t
ORIGIN      
        1 gaagccgctt gccggcgtcc gcttggaccg cggcggcggc gcacaggtcg tcgtacttct
       61 ggttcttcca atccgaatca ttgttgtcgc tgaagctcag gtacttgtcg gtgaacgtcg
      121 agacgtcgtt gtaatcgccg aaccagctgg cgcgggcgat cgcatagtcc ttcttgtgca
      181 gccgctcgcg gaaagtcttg atctcgaccg gctcgggctt gaagtcgatg ccgaggtgct
      241 cgatccactg gcggcggaca ttttgcgcga tgtcgccgtg gttcgcttcg ttgttgtaca
      301 gcagcgacag ctgcgggaac ccgcggccgc cgggataccc tgcgtcgtta agcaactgtc
      361 tggcgcgcgc gatgtcgtaa cctaatcccg gggctgattc gaaatggttg aatatgccgg
      421 gcgggatgta tttgtcggcc ggcttttcgc ccaaccgcgt gatcgtttcg acgatcgcct
      481 gcttgtcgat ggacatcgtc agcgcctgcc gcacgcgcgc atcggccaac ggattgtccc
      541 ggccatcagg caatttcggc tggcaattta ccgaataaaa gtacgtgccg aagctgggaa
      601 acacgtgcag gtccggccga tgcgcgcgga gcaactcggc ggcgatgtcg ccggtgatct
      661 ccgcctgcca gtccaccgcc ccgctgtcgt atttcagaaa tgccgccagc ggatcgtcgg
      721 cagacaccat gtagatcact ttgcttttga cgttagcgtg gtcccaatag ttagggttcg
      781 cttcgaagcg cacgccgcgt ttgaactgcc aatcgaccag gttgtacgga ccgttgccga
      841 cgaggtgccg cgtgaaggct ttgtcgtagg tgacgtggcc agtcggttca tccttctttt
      901 gaaatggccg catcgatcgc tcgttgagcg ggaagaacgg cgcgaacgcg cacaggtcgg
      961 ggaaaaacgt caccggatga ttcagcgtga cgcgcagcgt gtgcgggtca agcgcctcga
     1021 tgcctacgct ggcaaaatcc gcaggcttgt tgtccacgaa gttttgcgaa tacgccttgg
     1081 cgcctttgat gtagttgagc aggtacgagt aatcgcccgg ctcttccagc atccgccgcc
     1141 aggcgaacgc aaaatcgcca gcgacgacgg cgtcgccgtt gctccatcgg ccatcgctgc
     1201 gcagatgaaa cgtgtagacg gtcttatcgg ggttgatgtc gatcgactga gccgcgcccg
     1261 ggaccggatc gagcgtcacc gggtcgagcg cgtacagacc ttcccagatc atgtagccca
     1321 cccggatgtc ctgcatccag gacatccggt tgggatcgag cgtgccaatc tcaccgcgat
     1381 tgatgaaggt gaagtcgggg cgggatcggg cgcccccacc gcctgaccat gccagcgcag
     1441 caaccagtag cgccagaaga acgagcggaa tgacgagcag tcggcccatg cggatggacg
     1501 accggtatac gtcgtgtcca tggctggatc aatgccacgc gtggcgcccg cgagggaatt
     1561 agcgcgtgca cagtgaaccg cgttcgaatc gtgattacac ggagatcagt gcgtgggcgc
     1621 ggcggtggtc ggtgcggccg cggtggtcgg ggccgccagc gtcaccggcg ggggcgcctt
     1681 caggtccttg aactgcaact gcatcgccgc gaagacgccc ttggtcttct gcgccacctg
     1741 cgggcctggc gtgttgacgt ttaactgccc gatcggcgcc aacaccgtct gcacggccgg
     1801 ttggttcggc gaggtgacga tcagcgtgcc cgccgcgtac ttcagcaccg ggatcagttc
     1861 ctgcaactgg gccttggtcg cgttctgcgc ttgctgcccc gccacttcga tcaggttcac
     1921 cagcgactgc acaatgcgca gctgctgctt ggggttggcc ccgccatagc tacgcgagaa
     1981 aaacgtcggc agccgagact cggcactcgg ttgcggtgga atcgacgcgg tgtacagctg
     2041 gatgcggaaa tccagcaagt ccaggatcgg atcgagcacg aacggcatgg ccgccgtcac
     2101 ctgatccgcc ttcagcgtcg gcgacgcctc gggcatcagc gcatcgtacg catcggcaat
     2161 caaataaccc ggcgcttcgt cattaggaaa cgcgggcttg gcatgcttgg tcactgccgg
     2221 cttgatcgcc atcaccagct tgtcggctgc gatctgggca ggggtttgat acaccagctc
     2281 cagcacaggc ttggccgcct gcatcgcctt gatcgcgacg ggctcaagcg gatcgttgat
     2341 caagtcgatc acggcgggtt caaccttcgt tgtcttcccg acagtcgcca cgcgttcgac
     2401 gatgacggcc atcgcgagcc gcgcccgcag cgacttgccg gcgggttgct tcagcaggtt
     2461 ttggatttcc tggctgagca cctgcgcgta gacatcgaaa aacgattgcg tcgagccggg
     2521 ggtacattcg ttgatcaggc gctcgcgggc cgccttctgg gcggccgcgt cttcgccgcc
     2581 cagctgctgc aattgtccct ggatgaactg ctgaatctgc gggatgaagg cagcggcgtt
     2641 cggcgcgttc ttaatggtcg tcggcagcgt gggaccagcg gcggcggtcg cgccggcgcc
     2701 accaccaatc gatggcaacg acggcgctgc cggctgggcg agggcaaccc cgccagccaa
     2761 ccagaggccg gataacgcga tcaacccagc gcgggcgacg cagaccgagg cgggaatgcc
     2821 cacgcggtgg cggacgctaa cacggcggtt catcaggcgg ttctccggcg gatgcttgca
     2881 tcgtcgtcgt tgctcgttgg ccatcatccg cgccctaccc cttacgtgca cgcggcgcgc
     2941 gccggccaa
//
LOCUS       DATIMH010000100         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1756418, whole genome shotgun sequence.
ACCESSION   DATIMH010000100 DATIMH010000000
VERSION     DATIMH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756418"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VLI90_01750"
     CDS             complement(<1..293)
                     /locus_tag="VLI90_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HSV12955.1"
                     /translation="MPRTPIHSPHAPKAIGPYSQAIRSGNTLYLSGQTPLDPASGEMV
                     AGGIEEQATRVFENLKAVLAAAGASFEDVVRVAIYMTDLVNFATVNDVMKRYF"
     gene            complement(512..>1655)
                     /locus_tag="VLI90_01755"
     CDS             complement(512..>1655)
                     /locus_tag="VLI90_01755"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HSV12956.1"
                     /translation="KCRIAVRALPVTAKSSHCGFGVADGAVTISTVCPEASSARKGAS
                     TPSSLEFLENVKIDLFPDEVYLFTPRGDILSLPKNATALDFAYAVHTDVGHHAVASRV
                     DGALAPLRALLASGQTVEIVTAPSATPNPQWLDFAVTGKARTAIRHFLKRLQHEDAVE
                     FGHRMLDRALDAVGSSLDSIPAEALEKYLQQNSQRRLEELLSDIALGNRTPDQVAAQL
                     IALHGTAAGAKPRAREKIRITGAERGILSFGNCCHPLPGDTIVGYLSPGKGIVVHRTE
                     CPNLGELSKLPERRVEIEWDRDVQGDYKAQLRSEVVNRPGVLATVAAAIAAAGSNIEN
                     VEYTERSPFAATLLFTLEVKDRKHLADVMRRVRRTGVVNGVYRYPV"
BASE COUNT          270 a          548 c          529 g          308 t
ORIGIN      
        1 tggaagtagc gtttcattac atcgttgacg gtggcgaaat tgaccaggtc cgtcatatag
       61 attgcgaccc gcaccacgtc ctcgaacgac gcgccggccg cggccagcac ggccttgaga
      121 ttctcgaaca cgcgcgtggc ctgttcctcg attccgcctg cgaccatttc gccgctcgcg
      181 ggatcgagcg gcgtctgccc tgacaggtac agcgtgttgc cgctgcggat cgcctgcgag
      241 taggggccga tcgccttggg agcgtggggg gagtggatgg gagtgcgggg catggcgtct
      301 ggactatcag ttgattcagt ttgtcagtgt aaccggggat tgggggcggg tagcgggggc
      361 gccctgccct gcctttcccg ctagcactga agaactcgca gattgcatct tgaggtgccg
      421 caattttcaa tagcggtccg attgttgcgc cccgctaccc atcccccgct ttcgggacag
      481 agcgccctcg ctacccatcc gcgctcccgc tctacaccgg ataccggtaa acgccattga
      541 ctacccccgt cctccgcacc cgccgcatca cgtcggcgag atgcttgcga tccttgacct
      601 ccagcgtgaa cagcaaggtc gcggcgaagg ggctgcgttc ggtgtactcc acgttctcga
      661 tgttggagcc cgccgccgcg atcgccgcgg caacggtcgc cagcacgccg gggcggttga
      721 ccacctcgct gcgcaattgc gccttgtaat cgccctgcac gtcgcgatcc cattcgattt
      781 cgacgcgccg ctccggaagt ttggacagtt cacccaggtt cgggcactcc gtgcggtgca
      841 ccacgatccc cttgccgggt gacagatagc ccacgatcgt gtcgcccggc agcggatggc
      901 agcaattgcc gaagctgagg atcccgcgct cggcgccagt gatgcggatc ttctcgcgcg
      961 cccgcggctt ggcgccagca gccgtgccgt gcagggcgat cagctgcgcg gcgacctggt
     1021 cgggcgtgcg gttgcccagc gcaatgtcgg agagcaattc ctccagcctg cgctggctgt
     1081 tttgttgcag gtatttttcc aacgcctcgg ccgggatcga atcgaggctg gaacccactg
     1141 catccagcgc gcgatcaagc atccgatggc cgaactccac cgcatcttcg tgctgcaggc
     1201 gcttcaggaa atggcggatt gcggtgcgcg ccttgccggt gaccgcgaaa tccagccatt
     1261 gcggattcgg cgtcgccgaa ggcgcggtga cgatctcgac cgtctggccc gaagcgagca
     1321 gtgcccgcag cggcgccagc gcgccgtcca cccgcgaagc gacggcgtga tgccccacat
     1381 cggtgtgcac cgcgtaggca aaatccagcg cggtcgcgtt cttgggcagc gacaggatgt
     1441 cgccgcgcgg ggtgaacaga taaacctcgt ccggaaagag atctatcttg acgttctcca
     1501 ggaactccag cgacgacggc gtgctggcac ccttgcgcgc gctgctcgct tccggacaga
     1561 cggtggagat cgtcaccgcg ccgtcggcca cgccgaatcc gcagtggctg gacttcgcgg
     1621 tcaccggcaa ggcacgcacc gcgatccggc atttc
//
LOCUS       DATIMH010000101         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3200401, whole genome shotgun sequence.
ACCESSION   DATIMH010000101 DATIMH010000000
VERSION     DATIMH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3200401"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3127)
                     /locus_tag="VLI90_01760"
     CDS             complement(<1..>3127)
                     /locus_tag="VLI90_01760"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSV12957.1"
                     /translation="KFVATTAPAHPYNETNSPSFPNGGGVYNGSVTINSNDPNTSSKI
                     VPLAGWWQQQSENNDEPSLQSLVNLMNGWGTTINPAAGVPELTESGTTPTYYGEEEPF
                     GPNRSPTITPAPPTYWTVADAAYSVSVRQLSSWHTQGNTVQTWWFNQGSFSTHSLFTT
                     SADYGQTLFPVLNGTTTPAANSFSPGTAVFGFKVDSEWSDDTKNNTSGGGHHIRFYPL
                     RDASGQFVPNTYIMGMDYAVSGSQNFDFQDNVYVISNIKPATILAAPTDLYGVGGNGS
                     ATLQWAPVPGTVQSYNIYRSLAPSSGFALLTSGLTSTSYVDNSAPPGTQVYYRVSALN
                     NGLESLASTAAVTTTGTPAGSLQSIDIAAVPTGSTSVDTTNPANPIYTVVAGGPGATG
                     NSDGFRYLFEQQSGDFDVRLQVSSLTVAGSFSTAGLMARTSLNANSANVYMSASPVNY
                     RFKSRSTTGGTTTITAGANTAFPGAWVRLRRVGNVFTGFSSADGVNWTQLKQVSVTLP
                     STVFLGLAVASNNTTNTTTAQMSSFGPAFTGPVANADTFNVTANTSTPLDIFANDTDA
                     TGTIDRTSVVLSTPNQGGSVAFNPSTGLIDYTPASGFVGTETFTYTVSDNTALTSGSA
                     TVTVIVAPTGPTAHDDQFTTIAGQALPVNVVANDTDTTGTIDPTTVAIVTAPDQGGAA
                     VVDHTNGMITYTPAAGFIGSETFTYIVGDSNGLTATATVTMLVTSSPGAPVANPDNAN
                     TAVGQSVQINVLSNDVPDLTSGSALDPASVVTSDSVNGGSLQLDPTTGIINYTPPLGF
                     SGTDTFTYTVSDLSGHTSNSATVTVTVTPNGPVTADDSATAITGSSTKINVLNNDSST
                     APLNPASVAVASVPSHGAAVANADGTITYTPAAGFVGRDSFTYTVANNNGSTSNTAVV
                     SVDVGMTISSARGATHVLSWTDGDGTTALLTLNSGSANLFFTNGGTVTTTPAGRGTIG
                     GGANAISRMTISGTTAASVLVLRGTGGNGLVALPDVSSTTPLASIIAPAASLSGTFNI
                     AGLGVLLLGNAS"
BASE COUNT          466 a          973 c         1089 g          599 t
ORIGIN      
        1 cgctggcgtt gcccagcagc agcacgccga ggcctgcgat gttgaacgtt ccgctcaacg
       61 aagccgccgg cgcgatgatc gacgccagcg gcgtcgtgct gctgacgtcc ggcagcgcga
      121 ccagcccatt accaccggtg ccgcgaagca ccagcacgct ggcggcggtg gtaccgctga
      181 tcgtcatccg gctgatcgcg ttcgccccgc cgccgatcgt cccgcgaccg gccggcgtag
      241 tcgtcaccgt cccgccattg gtgaagaaga ggttggcgct gccgctgttg agcgtcagca
      301 gcgcggtcgt gccgtcaccg tcggtccagc ttagcacgtg cgtcgcgccg cgtgccgacg
      361 agatcgtcat gccgacatcc acgctgacca ccgcggtgtt ggacgtgctg ccgttgttgt
      421 tggcgacggt gtacgtgaac gaatcgcggc caacaaaccc tgcggccggc gtgtaggtga
      481 tcgtgccgtc ggcgttcgcc accgcggcgc cgtgcgatgg aaccgaagcg acggctaccg
      541 acgcggggtt gagcggcgca gtcgagctgt cgttgttcag cacgttgatt ttcgtgctgg
      601 aaccggtgat cgccgtcgcg ctgtcgtcgg ccgtcaccgg accgttcggc gtaaccgtca
      661 cggtgacggt ggccgagttg gacgtgtgac cggacaggtc cgacacggtg taggtgaacg
      721 tgtcggtccc gctgaatccc agcggcggcg tgtaattgat gatgccggtc gtcgggtcca
      781 gctgtagtga gccgccgttg acgctgtcgc tggtgacgac cgacgccggg tcgagcgcgc
      841 tgccggacgt caggtccggc acgtcgttgc tcagcacgtt gatctgcacg ctctgcccca
      901 cggcggtgtt ggcgttgtcg gggttggcca ccggcgcgcc gggactgctg gtcaccagca
      961 tcgtgacggt cgcggtcgcg gtcagcccgt tgctgtcgcc gacgatgtac gtgaacgtct
     1021 cgctgccgat gaaccccgcg gccggcgtgt aggtgatcat cccgttggtg tgatcgacca
     1081 ccgcggcgcc gccctgatcg ggcgccgtga cgatcgccac cgtggtagga tcgatcgtgc
     1141 cggtcgtatc tgtatcgttg gccaccacgt tcaccggcag cgcctggccg gcgatggtcg
     1201 tgaactgatc gtcatgcgcg gtcggcccgg tcggcgcgac gatgacggtc accgtcgccg
     1261 aacccgacgt cagcgcggtg ttgtcgctca cggtgtacgt gaaggtttcg gtgccgacga
     1321 aaccgctggc gggcgtgtag tcgatcaagc cggtgctcgg attgaacgcg acgctgccgc
     1381 cctggttcgg cgtggacagc accacgctcg tgcgatcgat cgtcccggtg gcgtcggtgt
     1441 cgttggcaaa aatgtccagc ggcgtcgagg tgttagccgt cacgttgaac gtgtcggcgt
     1501 tggccaccgg cccggtgaac gccgggccga agctgctcat ctgcgcggtc gtcgtgttgg
     1561 tcgtgttgtt cgacgcgacg gccagaccca ggaagacggt gctcggcagc gtcaccgaca
     1621 cttgcttcag ctgcgtccag ttcacgccat cagcgctgga gaagccggtg aagacgttgc
     1681 cgacgcggcg caggcgcacc catgcgccgg ggaacgccgt attggcgccc gcggtgattg
     1741 tcgtcgtgcc gccggtcgtc gatcggctct tgaagcgata gttcacgggg ctggcgctca
     1801 tgtagacgtt agcgctgttg gcgttgagcg atgtgcgagc catcaacccc gcggtggaga
     1861 acgagccggc aacggtcagc gacgacacct gcaggcgcac gtcgaagtcg ccactctgct
     1921 gctcgaagag atagcggaag ccgtcggaat tgccggtcgc accgggcccg ccggcgacaa
     1981 ccgtgtagat cgggttcgcc ggattagtgg tatcgacgct ggtcgaacca gtcggcacgg
     2041 cggcgatgtc gatgctctgc agcgacccgg ccggcgtgcc ggtcgtcgtc accgcggcgg
     2101 tgctggccag cgactcaagt ccgttgttga gcgccgatac gcggtaatac acttgcgtgc
     2161 ccggaggcgc gctgttgtcg acatagctcg tcgaagtcag accgctggtc agcagcgcga
     2221 acccgctgct gggcgcgagg ctgcgataga tgttgtagct ctgcaccgtc cctggcaccg
     2281 gcgcccattg cagggtggcg ctgccgttgc caccgacgcc gtacaggtcc gtcggcgccg
     2341 ccaggatcgt cgccggtttg atgttgctga tgacgtagac gttgtcctga aagtcaaagt
     2401 tctgactgcc cgagaccgcg taatccatcc ccatgatgta cgtgtttgga acgaactgcc
     2461 cgctggcgtc gcgcagcgga tagaagcgga tgtgatgccc gccgccgctg gtgttgttct
     2521 tggtgtcgtc gctccattcg ctgtccacct taaagccgaa gacggccgtg cccggggaaa
     2581 acgaatttgc cgcgggcgtg gtggtgccat tgaggacggg gaagagcgtc tggccgtagt
     2641 cggccgacgt tgtgaacagc gagtgcgtgc tgaacgagcc ctggttgaac caccaggtct
     2701 gcacggtgtt cccctgcgtg tgccaggacg acagctgccg caccgacacc gaataggcgg
     2761 cgtcggcgac tgtccaatac gtcggtggcg caggcgtgat cgtcggcgat cggttcgggc
     2821 caaacggttc ctcttcaccg taataggtcg gcgtggtgcc gctctcggtc agctccggca
     2881 cgcccgccgc ggggttgatc gtcgtgcccc aaccattcat gaggttgacc agcgattgca
     2941 ggctcggctc gtcgttattc tccgactgct gctgccacca gccggccagc gggacgatct
     3001 tgctcgacgt gttcgggtcg ttgctgttga tcgtgaccga gccgttgtac accccgccgc
     3061 cgttggggaa gctgggcgag ttcgtctcgt tatacggatg cgccggcgcg gtggtggcga
     3121 cgaactt
//
LOCUS       DATIMH010000102         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_313741, whole genome shotgun sequence.
ACCESSION   DATIMH010000102 DATIMH010000000
VERSION     DATIMH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313741"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="VLI90_01765"
     CDS             <1..1544
                     /locus_tag="VLI90_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12958.1"
                     /translation="TSPTTAAAARDPRIGEMYDYYATLYAQPLNQKDRLARLVGIVSL
                     SRIDGPPLTRALLGVLKDDDLVVGQFAWEGLHARQASLVDRDRTAWLDGGMDLARRGA
                     FPGATAAPLLAALIERQPGTLSGINEFLERVAEENDPATTGGRAALDAGSKTIAVWQD
                     TKILQALVARMAAKPRFTSRLARLLSGLPDAPAVKDADAAAVRSAWSKYAQHAKFAPP
                     TTLPAYTGFSAVLPKAGAIKDVFGKKWHKELELPKLKIDSVEIAFCVDATPSMAVSNP
                     YVATYIQTVAQLFNLLSYNVRCGAVYYRHEIDPELMDACCKRWQNFEGDFAVKVMPLE
                     SAGDLVAKMREMKIGGRGTGHSGNGAYVAGIETAMRGMAWTAKSKRVIALTGDAKPTP
                     NTAKALVDIATKAKADGYTLIFIERDRYAAAAVDDASRAALGMSPILYGDDIKKLQGD
                     AANDAFAAIAAFHDTAFEQMAARVLQQSLPEPYRDRAAALMKMMVGLLQAKAAAAHAA
                     AVAKG"
     gene            1866..2333
                     /locus_tag="VLI90_01770"
     CDS             1866..2333
                     /locus_tag="VLI90_01770"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12959.1"
                     /translation="MKGIPIGQLLVEQGVLTPRQVEHILTVQKQSHRPFGDLAERLFG
                     INPRAVEDAWVEQYTRTVGIVDLDEQQMDEDCLRLLNRRQAWQFHTLPTHREEDNLNL
                     ATTPENLVRAVNFATRKIDEPICFLLAERQQLREFLMKHYPVPEHIAEFASKM"
     gene            complement(2611..3186)
                     /locus_tag="VLI90_01775"
     CDS             complement(2611..3186)
                     /locus_tag="VLI90_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HSV12960.1"
                     /translation="MIAGGAPTVLASFDGGAHGSQGGGEVLVVGNTIYGTTVEGGVNG
                     DGEVFSLPIAGGTPTVLASFDGNDGLDPEGPITLSADDKTIYGTTVRGGPDNVGVVYS
                     VPITGGTPAVLASFNVTNGPELGLALSNGILYGTTELGGANGAGTVYAVVPEPACASV
                     MLLISGVMLCGRRRVRTLSWAEHVLVRSRSI"
     gene            complement(3528..>3731)
                     /locus_tag="VLI90_01780"
     CDS             complement(3528..>3731)
                     /locus_tag="VLI90_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12961.1"
                     /translation="MAGATDGIAAVENRTLAGKIIVYPMLHDLGLTPLTDLSKKFPTV
                     AAKLSNGQWTKAAEAELLSVAAK"
BASE COUNT          703 a         1234 c         1190 g          604 t
ORIGIN      
        1 ccacgtcgcc aacgacggcc gccgcggcgc gcgatccgcg catcggtgag atgtacgact
       61 actacgcgac gctctacgcc caaccgctca accagaagga tcgcctcgcc cgccttgtcg
      121 gcatcgtctc actctcgcgc atcgacggcc cgccgctgac gcgggcgctg cttggcgtcc
      181 tgaaggacga cgacctcgtc gtcgggcagt tcgcgtggga aggcctgcac gcccggcagg
      241 cgtcgctggt cgatcgcgat cgcaccgcgt ggctcgacgg cggcatggac ctcgcccgcc
      301 gcggggcgtt tcccggcgcc accgcggcgc cgctgctggc ggcgctgatc gagcgacagc
      361 cgggcacact gtctggcatc aacgagtttc tcgagcgcgt cgccgaagaa aacgacccgg
      421 caacgaccgg cggtcgcgct gcgctggacg cgggcagcaa gaccatcgcg gtctggcagg
      481 acacgaagat cctgcaggcg ctggtcgcgc gcatggcggc caagccgcgg ttcacgtcgc
      541 ggttggctcg cctgttgagc ggcctgcccg atgcgccggc ggtgaaggac gccgacgccg
      601 cggcggttcg ctcggcctgg tcgaaatatg cccagcacgc caagttcgcc ccgcccacca
      661 cgctgccggc gtacaccggc ttcagcgccg tgctcccgaa ggcgggagca atcaaagatg
      721 tgttcggtaa gaagtggcac aaggaactgg agttgccgaa gctgaagatc gacagcgtcg
      781 aaatcgcatt ctgcgtggac gccacgccaa gcatggcggt gtccaacccg tacgtggcga
      841 cgtacatcca gacggtcgcg cagctgttca acctcctgtc ttacaacgtg cgctgcggcg
      901 cggtttacta tcgccacgag atcgaccccg agctgatgga cgcctgctgt aagcgctggc
      961 aaaatttcga aggcgacttc gccgtcaagg tgatgccgct cgaatccgcc ggtgatctcg
     1021 ttgcaaaaat gcgcgagatg aagatcggcg ggcgcggcac cggtcacagc ggcaacggcg
     1081 cgtacgtcgc cggcatcgag accgccatgc gcggcatggc gtggaccgcc aagagcaaac
     1141 gcgtgatcgc cctcaccggc gacgccaagc ccacgccgaa caccgccaag gcgctggtgg
     1201 acatcgcaac caaggcgaag gcggacggat acacgctgat cttcatcgag cgcgaccgct
     1261 acgccgccgc cgcggtggac gacgccagcc gggcggccct gggcatgtcg ccgatcctgt
     1321 acggcgacga cattaaaaag ctgcaaggcg acgcggccaa cgacgcgttc gccgccatcg
     1381 ccgcattcca cgacaccgcg ttcgaacaga tggccgcccg cgtgctgcag caatcgctgc
     1441 cggaaccgta tcgcgatcga gcggcggcgc tgatgaagat gatggtcggc ctgctgcagg
     1501 ccaaagccgc cgccgcccac gccgccgcgg tggcgaaggg gtgaacgaag gacgtgctgg
     1561 tgcgcagttg aaggctctaa ccgcgaaaca acggatcctt ccgttgcgca atgacagccg
     1621 cttgcggctt agcatcgttt ttcgtccgaa acagacgatt aagccgcaag cggctctgac
     1681 gcgctttttg cgaacgagca attcatcgac tcctcggcca gatccttcga ctccggcgag
     1741 tacgcctgcg ctggaggatc actgctaacc aaatcttatc ggcccccaca ccgcgcagcc
     1801 aaactcaagc cacaatccaa ccgatacgaa cagagaccgg caaccggtcc actggaggtc
     1861 atgcggtgaa gggtattccg attggacaat tactggtcga acagggcgtg ctcacgccgc
     1921 ggcaggtcga gcatatcctg acggtgcaga agcagtcgca ccgccccttc ggcgacctgg
     1981 ccgagcggct gttcggcatc aacccgcggg cggtcgaaga cgcgtgggtg gagcagtaca
     2041 cccgcacggt cggcatcgtg gacctggacg agcagcagat ggacgaggat tgcctacggc
     2101 tgctaaaccg ccggcaggcg tggcagttcc acacgctgcc aacgcatcgc gaggaagaca
     2161 acctgaacct ggcgacgacg ccggagaacc tggttcgcgc ggtgaatttc gcgacgcgga
     2221 agatcgatga accgatctgc ttcctgctgg ccgagcggca acagctgcgg gagtttttga
     2281 tgaagcacta cccggtgccg gaacacatcg cggagttcgc gtcgaagatg tgaggcgcct
     2341 cgcgcgcatc aaccaacgga attgaagcag agcctcgcac gtgggtgtgc gaggctctgt
     2401 tgttttctgc gttcgtgtgt gagtttatcc tgagcggcgc agccgcgaag tcgaatcatc
     2461 tggccaagaa gtagatgaac gtcgagctac gaaagcggca acgcagccgc ttgcggcttc
     2521 gcagacccag aaccgcggct tgtccgtctg ccaaaccgca agcgactgcc gcgcccattc
     2581 ggcgcgagca gcggttctcg gacgcaccgg ctaaatgctt cgactccgca cgagtacgtg
     2641 ctccgcccag gataaagtgc gaacgcgccg tcggccgcac aacatcacgc cgctgatgag
     2701 cagcatgacc gacgcgcacg ccggttccgg cacgaccgca tagacggtgc cggcgccatt
     2761 ggcgccaccc agttcggtcg tgccgtagag gatgccattg gacaaagcca aacctagttc
     2821 ggggccgttg gttacgttga agctcgccag aacggccggc gtgccgccgg tgatcggcac
     2881 ggaataaacc acaccgacgt tgtccggccc accgcggacg gtggtgccgt agatcgtctt
     2941 gtcgtcggcg gacagcgtga tggggccttc ggggtccagg ccgtcattgc cgtcgaacga
     3001 cgcgagcacc gtcggtgttc cgccggcgat cggcagcgag aacacttcac cgtcaccgtt
     3061 gacgccgcct tcgacggtcg tgccgtagat cgtgttgccg acgacgagga cttcgccccc
     3121 gccctggctc ccgtgtgcgc caccgtcgaa cgacgccagc accgtgggcg cgccgccggc
     3181 aatcacgcag cgatttcgcg acgggactcc aggggtgcgg cgtttgattt tgatagatcg
     3241 tctctgcgcc aagacgccaa gacgccaagg aattaaaggc aatggcttac tggtaacacc
     3301 ggctccgagg ctatggctct ctcggccgtg ccaggcactt acggcgcggg cgaaacgccc
     3361 gtgctgcgcg ccgagcactt cacaggttcc tgtcgttctc ttccttggcg ccttggcctc
     3421 ttggcgtctt ggcggagata gacgcatacc aagatcgtgc gctacacccg gccgcaggcg
     3481 agcaaggata aggcgatgcg cgtgaagcgc tggcgctgcg cgttacctca cttcgccgcg
     3541 acgctgagca gctccgcttc ggcggccttc gtccactggc cgttgctcag cttcgccgcc
     3601 accgttggaa acttcttcga caagtccgtc aacggcgtca gacccagatc gtgcagcatc
     3661 ggatacacga tgatcttccc cgccagcgtg cggttctcca ccgcggcgat gccgtcggtc
     3721 gcgccggcca t
//
LOCUS       DATIMH010000103         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1468310, whole genome shotgun sequence.
ACCESSION   DATIMH010000103 DATIMH010000000
VERSION     DATIMH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468310"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VLI90_01785"
     CDS             <1..873
                     /locus_tag="VLI90_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12962.1"
                     /translation="ATAARRFITFWLAHQNHQPDRFGVAGTTYDLDYLRDGEKLEPHL
                     YDKSSPPNTGGPGYDAADADPPIIAITAARYFRATHDLDLLKSHADNWQLTEHAMTAM
                     LDRDGLTWAHPKYRIKYLMDAVEAQVGFAALADIWQALGDDAKSVHTRALSNQMRRGI
                     ASLFSEKTACYDWNRDEAGAQQSCDWHNVYPDTMEQLWPAVWGVEDARVPHSQAAWSA
                     FVQHWPQWSSQNQMLNWPHTAVVASHLDDADQKQAREQIDRILSQRMNDDAWEVNQMY
                     FTMLAIAGETDGRD"
     gene            860..2659
                     /locus_tag="VLI90_01790"
     CDS             860..2659
                     /locus_tag="VLI90_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12963.1"
                     /translation="MGEINKPRSRTILADYFLGFATRAVSGGARPWRLYYETEGDAGL
                     KPPRCDAAFSGDLYVTGDIKDIASTYYPAIGPYDVTDPDVAEYHVLLAKAAGIDAFMA
                     EYTMGQEAQLLSLVQAARTYNFCIGVNWITQSHLAENTWRDRDEAMAKAHELVRWMAK
                     HVYEPCGVRVDGRYLLMIFLAHPREPAKPFDPFFSPDEVRALKRTALEAGCDANFLVL
                     PWEPLPESPAPAPARPGERVWDGYFPWVLRLNEPVPADQRWTHASSRAGFIERMQTYY
                     ATSRRLRDEGRIGTYIGGVCPGFDDHKGQAWGEGHRRHLPRDAGQTLADSWKAMDEAG
                     VDTGMIITWNDWAESSQIEPSLELGSNDLIECARQIARWKQTNSDPRLLAMPLRLFTA
                     RKDVWLLECAGVHLYETRVANEVLNRVALAIAHLDPIHAAELLGQAVAHIDAIKERLH
                     TAAVHAFWEFGFNAAGIAAVNNDPITTTTVENFTGIAFDASASTIGFAVTEEVRQTIQ
                     RGHLVGRLCVEYLDTGVDFVQVLVDAPDDAHRVIASFKKRNTGKWQRASMELVNARFT
                     RGLDGGADITITQRPGTTGGVRMVRIDGTVYGL"
     gene            2907..3377
                     /locus_tag="VLI90_01795"
     CDS             2907..3377
                     /locus_tag="VLI90_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12964.1"
                     /translation="MKRTLLNLAAAASLLLSLAIALLWVRTYVAQDQLSWRHGPYFAM
                     AETYAGVVEISLSAWMNFRHVPDGWQFFSGPYHDPYEHLAIHRQRPPPGRSDWDRWLI
                     VLPMWLILLPSALPLVAWWRWRQRAARAGTCPRCGYDLRATPDRCPECGMITGR"
BASE COUNT          636 a         1236 c         1031 g          537 t
ORIGIN      
        1 gccaccgccg cgcgacgctt catcacgttc tggctcgccc accaaaacca ccagcccgat
       61 cgcttcggcg tcgccggcac gacgtacgat cttgactacc tgcgcgacgg cgagaaactc
      121 gagccgcacc tctacgacaa gtccagccca ccgaacaccg gcggccccgg ttacgacgcc
      181 gccgacgccg acccgcccat catcgccatc accgccgccc gatattttcg cgccacgcac
      241 gatctcgatc tgctcaaatc ccacgccgac aactggcagc tgaccgagca tgccatgacc
      301 gcgatgctcg accgcgacgg gctcacctgg gctcacccga agtaccgcat caagtacctg
      361 atggacgcgg tcgaagcgca ggtcggcttc gccgcgcttg ccgacatctg gcaggcgctc
      421 ggcgacgacg ccaaatccgt ccacacccgc gcgctctcga accagatgcg ccgcggcatc
      481 gcctcgctct tctccgaaaa gactgcctgc tacgactgga accgcgacga agccggggcg
      541 cagcagtcgt gcgactggca caacgtgtat cccgacacga tggaacaact ctggcccgcg
      601 gtgtggggcg tggaagacgc gcgcgtgccg catagccagg ccgcgtggtc ggcgttcgtc
      661 cagcactggc cgcagtggtc gtcgcagaac cagatgctca actggccgca caccgccgtc
      721 gtcgcatcgc atctcgatga cgccgaccag aagcaggcgc gcgagcagat cgatcggatc
      781 ctgagccagc gcatgaacga tgacgcgtgg gaagtgaacc agatgtattt cacgatgctc
      841 gcgattgctg gagagaccga tgggcgagat taataagccc agatcccgaa cgatcctcgc
      901 cgattatttc ctcggcttcg ccacccgcgc cgtctccggc ggcgcgcgcc cctggcgctt
      961 gtactacgaa accgaaggcg acgccggcct caaaccgccg cgctgcgacg ccgcgttttc
     1021 cggcgacctg tacgtcaccg gcgacatcaa ggacatcgcc tcgacgtact accccgcgat
     1081 cggtccgtac gatgtgaccg accccgacgt ggccgagtac cacgtgctgc tggccaaagc
     1141 ggccggcatc gacgcgttca tggccgagta cacgatgggc caggaagcgc agctgctcag
     1201 cctcgttcaa gcggcgcgaa cgtacaactt ctgcatcggc gtcaactgga tcacgcaatc
     1261 acacctggcc gagaacacct ggcgcgatcg cgacgaagcg atggccaagg cgcacgagct
     1321 ggtccgctgg atggcgaagc acgtgtacga gccgtgcggc gtgcgcgtgg acgggcgcta
     1381 ccttctgatg atcttcttgg cccacccgcg cgaaccggcg aagccgttcg atccgttctt
     1441 ctcacccgac gaggtccgcg cgctcaaacg taccgcgctg gaagccggct gcgacgccaa
     1501 ctttctcgtc ctgccgtggg aaccgctgcc ggaatcgcca gcccccgcgc ccgcgcgccc
     1561 cggcgagcgc gtctgggacg gctatttccc ctgggtcctt cgcttgaacg aaccggtgcc
     1621 ggcggaccag cgctggacgc atgcctcgtc gcgcgccggt ttcatcgagc ggatgcaaac
     1681 ctattacgcc acctcgcgcc gcctgcgcga tgaaggccgc atcggcacgt acatcggcgg
     1741 cgtgtgcccc ggctttgacg accacaaagg ccaagcctgg ggtgaaggcc accgccggca
     1801 cctgccgcgc gacgccgggc agacgctggc ggattcgtgg aaagcgatgg acgaagccgg
     1861 agttgacacc gggatgatca tcacgtggaa cgactgggcc gaaagctcgc agatcgagcc
     1921 gagcctcgag ctgggcagca acgacttgat cgaatgtgcc cgccagatcg cccgctggaa
     1981 gcaaaccaac agcgacccgc gcctgctcgc catgccgctg cggctgttca ccgcacgcaa
     2041 agatgtctgg ctgctcgaat gcgccggcgt tcacctgtac gaaacccgcg tcgccaacga
     2101 ggtgctaaat cgcgtcgcgc tggcgatcgc ccatctcgat cccattcacg ccgccgaatt
     2161 gctcggtcaa gccgtcgccc acatcgacgc catcaaagaa cgcctgcaca ccgctgcggt
     2221 ccacgccttc tgggaattcg gcttcaacgc cgccggcatc gccgcagtga acaacgatcc
     2281 gatcaccacc acaaccgtgg aaaactttac tggcatcgcc ttcgacgcca gcgccagcac
     2341 gatcggtttc gccgtcaccg aggaagttcg ccaaacgatc caacgcggtc acctcgtcgg
     2401 ccggctgtgc gtcgaatacc tcgacaccgg cgtcgatttc gtgcaggtcc tcgtcgatgc
     2461 ccctgatgac gcccaccgcg tcatcgccag cttcaaaaag cgcaacaccg gcaaatggca
     2521 acgcgcgtcg atggaactgg tcaacgcccg cttcacccgc ggcctcgacg gcggcgccga
     2581 catcacgatc acccaacgcc ccggcacaac cggcggcgtg cggatggtgc ggatcgacgg
     2641 aaccgtgtac gggctctaac gcggcgggcg cacgcacgca cgctgtcgtc ctgagcgcag
     2701 gcgtgctcgc cgaaatcgaa ggatcttgcc aggaaatatc tgaagcgaat ctgtgcacaa
     2761 gggcgtggtc gtggcctgcc tttcgcggcg ccgcgaacgg gcaagccact tcattcgcgt
     2821 cattcctcta cgacggtcca ccgacgttcc caaatgaccc gcacaacttc cgcgggcatt
     2881 cgcaccatcg ttacaatatc ccgcgcgtga agcgcacgct gctcaacctc gccgccgccg
     2941 catcgctgct gctatcgctg gcgatcgcac tgctctgggt tcgcacctac gtcgcgcagg
     3001 atcaattgtc ctggcggcac gggccatact tcgccatggc cgagacctac gcgggcgtcg
     3061 tggagatctc gctcagcgcg tggatgaact tccgccacgt gcccgacggc tggcagttct
     3121 tcagcggccc gtatcacgac ccgtacgagc acctcgcgat ccaccgccag cgcccaccgc
     3181 cggggcgatc ggattgggat cgctggctga tcgtgctgcc gatgtggctc atcctgctgc
     3241 ccagcgcgct gccgctggtg gcgtggtggc gatggcgcca gcgcgccgcg cgcgccggca
     3301 cctgcccgcg ctgtgggtat gatctgcgcg ccacccccga ccgctgcccc gaatgcggca
     3361 tgatcacggg acgatagtcc gccggcgcgt gcgatcgatg caattgccga gacgccgaga
     3421 gcgccgaggc ggacgccgag
//
LOCUS       DATIMH010000104         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1757087, whole genome shotgun sequence.
ACCESSION   DATIMH010000104 DATIMH010000000
VERSION     DATIMH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757087"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1681)
                     /locus_tag="VLI90_01800"
     CDS             complement(<1..>1681)
                     /locus_tag="VLI90_01800"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HSV12965.1"
                     /translation="DWLDAARYADTHGYQVDPEKEMWPWRDWVINAFNRNLPYDRFTI
                     EQLAGDLLANATLDQKIATGFQRNHRINSETGSIAEEFQAENVVDRVSTMGAVWLGLT
                     VGCARCHDHKYDPITARDFYSFYAFFNTVDEIGNGGPRDGRGNHKPYLRLPAPELEAQ
                     AAAKEKEIVEARRLLDEVEKRLEPRQAEWESRALAEQARWEVLQPANLKAGGGVQLTA
                     QPDGSILASGAMPPSPVYEFTAATKLRNITGFRLELLPDQSLPASGSGRGPGGKGVVT
                     LFEVRNGDRKVDLARITTDFKSEESELDLVIRPADQLKRGWGVNPETTKPHYAVIEPA
                     RMFGSPDGTQLAIRIGNEYEGAPVGRFRVSVTDSEFPEVMPDEIRRILTTEAGTRSDK
                     DRGALRRFFLNHPRERRRASEVVTAREAEKRTIENKIPTTMVMQEMAAPRETFVLLRG
                     SYEKPGEKVTPAVPSFLPPMPAGAPANRLGLAEWLTDPAHPLTARVAVNRYWQSYFGT
                     GLVKTAEDFGAQGEAPSHPELLDWLATEFVRTGWDVKAMQRLIVTSATYRQQ"
BASE COUNT          257 a          529 c          543 g          352 t
ORIGIN      
        1 ttgctggcgg taagtcgcgg aggtgacgat cagccgttgc atggctttca cgtcccatcc
       61 cgtgcgaacg aactcggtgg ccagccagtc gagtagttcc ggatgactgg gcgcctcgcc
      121 ttgcgcaccg aagtcttccg ccgtcttcac cagaccggtg ccgaagtagg actgccagta
      181 gcggttcacg gcgacccgtg ccgtaagcgg atgcgcggga tcggtgagcc actcggcaag
      241 gcccagtcga ttcgccggcg cgcccgcggg catcggcggg agaaaggagg gaacggccgg
      301 cgtgaccttt tcgccgggtt tctcgtagct gccgcgcagc agcacgaatg tttcgcgcgg
      361 tgcggccatt tcctgcatca ccatcgtggt gggaattttg ttttcgatcg tgcgcttctc
      421 ggcctcgcgc gctgtcacca cttcgctagc ccggcggcgt tcgcgcggat gattcaggaa
      481 gaagcgccgc aacgcgccac gatctttatc ggagcgggtg ccggcttcgg tcgtcaggat
      541 cctgcgaatc tcgtccggca tcacctcggg aaactcggag tcggtaaccg agactcgaaa
      601 gcgtccaaca ggtgcgccct cgtactcgtt gccgatgcgg atcgccaatt gcgtgccatc
      661 cggtgagccg aacatcctgg ccggctcgat cacggcgtag tgcggcttcg ttgtctccgg
      721 attcactccc cagccgcgct tcagctgatc ggcggggcgg atgaccagat cgagctccga
      781 ttcttcgctt ttgaaatcgg tggtgatgcg cgccaggtcc accttgcggt cgccgtttcg
      841 cacctcgaag agcgtgacca cgcctttgcc gccgggtccc cggccgctgc cgctcgccgg
      901 caaactttgg tccggcagca gttccaggcg gaagccggtg atgttcctca gtttggtggc
      961 ggccgtaaat tcatataccg gggacggggg catggcgccg ctggcaagta tggagccatc
     1021 gggctgcgcc gtgagctgaa cgccgccgcc ggccttgagg tttgcgggct gtaggacctc
     1081 ccatcgcgct tgttccgcga gggcgcgcga ttcccactcc gcctggcggg gctcgagtcg
     1141 cttttcgact tcgtcaagga gccgcctggc ctcgacgatc tctttttcct tcgcagcggc
     1201 ctgtgcctcg agttccggcg cgggcagccg caggtacggt ttgtgattgc cgcggccgtc
     1261 gcgtgggccg ccgttaccga tctcgtccac cgtgttgaag aacgcgtaga agctgtagaa
     1321 gtcgcgcgcg gtgatcgggt cgtacttgtg atcgtggcac cgggcgcagc cgaccgtcag
     1381 gccaagccat accgcgccca tggtgctgac acggtccacc acgttctccg cctggaactc
     1441 ttccgcgatc gacccggttt ctgaattgat gcgatggttc cgttgaaacc ccgtcgcgat
     1501 cttctgatcg agagtggcgt tcgctaagag gtcgccggca agctgttcga tggtgaaacg
     1561 gtcgtacggc aggtttcgat tgaaagcgtt gatcacccag tcgcgccacg gccacatctc
     1621 tttttcagga tcgacctgat agccgtgcgt gtcggcatag cgtgccgcgt ccagccagtc
     1681 t
//
LOCUS       DATIMH010000105         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2912906, whole genome shotgun sequence.
ACCESSION   DATIMH010000105 DATIMH010000000
VERSION     DATIMH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2912906"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      311..509
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            813..1874
                     /locus_tag="VLI90_01805"
     CDS             813..1874
                     /locus_tag="VLI90_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12966.1"
                     /translation="MKPAPAVLAGAVALTALALLPNESRAQYVPPAGYTKTPLLTAAN
                     QPLNAAGIGVSGGGRLAIADGSTITLYNTWQNGRTVIGSITDSADWSFLTDITFIGEN
                     KIIVGENGKSKTAWSIDFTTPATPVAAKIAPSNSFPAIQDLALLNFTTALVSGSTASQ
                     GASGGLYLDKLNLTNGNISPVVSNAGSGFVGATGVTPAGSGVLLDTGVTTGIAHIYDS
                     TNGTFVRDVSFAGGGGTGAYGLAFDPAGDAFVTTGATLTEITGIDSANPAVTTFGSFS
                     EGAAGFPFPTGIAFTGDSFRPDDSAQTGVLLVNGDFTNDGTVFAITSVPEPASAGFLL
                     LAGVALVGQRRAKRGGDDV"
     gene            complement(1830..>2052)
                     /locus_tag="VLI90_01810"
     CDS             complement(1830..>2052)
                     /locus_tag="VLI90_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12967.1"
                     /translation="RVRLGLPWMGANLVLAALAWATAMQPQAGQAVAAGPAGAAKTAA
                     PASMATHRTLRTSVALHIITSALRSSLAY"
BASE COUNT          345 a          690 c          647 g          370 t
ORIGIN      
        1 cctggcgcga actacgcgtg agcctcgatc agcggcgagc cgccagagcc ttgcgacgcc
       61 gctttcggcg gaaccccgat cattatctgc gtcaggtgga acgcgaacta ctccagtcga
      121 ctttgccacc ctagaaaaaa agacatttac ctcgtgagta cactccgtgc gcggctcttg
      181 acgatcgccg aaactgtcac gcacccgaag tgaccaggcc ggagtgcgcg cgagtgcgcg
      241 cggcttgatt caattctcgc gctcgctatg ttgtccgcca agcccccggg cggctgcgaa
      301 gcccgttgcg agttttccaa ggagcatctg tttccttcca cactcgcgac gatgggaagt
      361 cggtgagaat ccgacgcgga cgcgccgctg tatgggaccg ctttgttgtg ccgtcgatca
      421 ttgagccact gctggcaacc aagccggtgg gaaggcgatc ggcgccaagg tcccgagcca
      481 gaagaccggc ctgtgggtga acggctgacg cgatgtgcgt gggccacgtt cggatacgtc
      541 ctcgcgattc gggacggccg gtttagcaag cagcccgcag gcaagcgact tctgttatcg
      601 ttttgcgacg cgcgcaaatc cgcgtcgcct cgtttgcatt tccgcgtgcg cgcgtgcttg
      661 atccccggtt cgtttcccac ggttgacgcc gtgccccgtt atcgcgaggg cgacggttct
      721 tcgttgcgtg ccgcgcgaat cggcatgcca gcgggctttg cccgcgggag actttcatgg
      781 ttcgggatcg agttgcttat cgttcgagtc gcatgaagcc ggcaccggcc gtgctggccg
      841 gtgctgttgc gttgactgcg ttggcgctgc tgccaaacga atcgcgcgcg cagtacgtgc
      901 cgccagctgg ctacaccaag acgccgctgc tcacggcggc taaccaaccg ctcaatgccg
      961 ccggtattgg ggtaagcggt gggggacgtc tggcgatcgc cgatggttcg accatcacgc
     1021 tctacaacac ctggcagaac ggccgcacgg tcatcggcag catcaccgac agcgccgact
     1081 ggagcttcct caccgacatc accttcatcg gtgaaaacaa gatcatcgtc ggcgaaaacg
     1141 gcaagagtaa aaccgcgtgg tcgattgact tcacaacgcc ggcgacgccg gtggcggcga
     1201 agatcgcgcc gtcgaacagc tttcccgcaa tccaagacct ggcgctgctc aacttcacca
     1261 ccgcgctggt cagcggttcc accgcgtcgc agggtgcgtc gggcgggctg tatctcgaca
     1321 aactgaacct gaccaacggc aacatctcgc cggtcgtctc caacgccggc tcaggcttcg
     1381 ttggcgcaac cggcgtcaca ccagccggca gcggcgtgct gctcgacacg ggcgtcacca
     1441 ccggcatcgc gcacatttac gactcgacca acggcacctt cgtacgcgac gtcagtttcg
     1501 ccggaggggg cgggactggc gcgtacggat tggcgttcga tcccgccggc gatgcgttcg
     1561 tcaccaccgg cgcgacgctt acggagatca ccggcatcga ttcagccaac cccgcggtga
     1621 cgacgttcgg ctccttctcc gaaggcgcgg ccggcttccc gtttcccacc ggcatcgcct
     1681 tcaccggtga ctcgttccgc cccgatgatt ccgcgcagac cggcgtcctc ctagtgaacg
     1741 gcgacttcac caacgatgga accgtcttcg ccattaccag cgtgccggag ccggcgtcgg
     1801 cggggtttct gctgctcgcg ggcgtcgcgc tagtaggcca acgacgagcg aagcgcggag
     1861 gtgatgatgt gtaacgccac gctcgttcga agcgttcgat gcgtggccat gctggccggc
     1921 gcggcggtct tcgccgcgcc ggccgggccg gccgccaccg cctgcccggc ctgcggctgc
     1981 atggccgtcg cccacgccag cgcggccagg accaggttgg cgcccatcca cggcaggccg
     2041 aggcgcacgc gc
//
LOCUS       DATIMH010000106         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2046169, whole genome shotgun sequence.
ACCESSION   DATIMH010000106 DATIMH010000000
VERSION     DATIMH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046169"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..2856)
                     /locus_tag="VLI90_01815"
     CDS             complement(232..2856)
                     /locus_tag="VLI90_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV12968.1"
                     /translation="MRTLRRSLARLLNFTVNRRGDERLREEIESHIAAQTDENIRAGM
                     AHHEARRQACLKFGAVAALTHDYNAEKGLPFVDNLLLDIRYSLRVLRKSPAFTVVALI
                     TLMLGIGANVVVFGVVNAVLLQPLPVKDPQSLYQLRHKRWMSGRLLTTSYPALEDFRQ
                     QNTTFSGLAGVYGYSNATLSWHNTALKVHGDEVTGNYFDLLGVYPRAGRLFRDGDENG
                     PGSAPYVVLSDTLWRSVFQSDPGVVGATVDLNKHPFTVIGVAPPGFHGAERFVWPDYW
                     IPMANQAQIDKSDYLHSRTAVTVTVLGRLKPGVTPQQATDNLNAISAGLAKIYPETDD
                     GQPLRLIHPGLYGDNGDVIRGFLWSVTALALLVLTAACANLATLFAARAADRGRELAL
                     RVALGSSRLRLVRQLLTEAVIVSLMGGAAGVVGAQLLLRLLNRSQPFAGALTVNVDAR
                     VYLAGLIFTLASALLFGMAPVRQLGRGTPLRMMKNGPPGAPRLRHVAMRDLLLGAQIA
                     ICTLLITASLVAVRGMVRVLRAPLGFQPRGAMLVELGDIPADKKKPVIEAARAIPGVT
                     SAGMVNRTPMTGGIHGIPIFRAGVNDFKLNNSALSSYVFQMSPGYLEAAGTRLLTGRD
                     FSWHDTADSPRVGIVNQSFARRMWGEAPPIGKYFVVSGNLTEVVGVAETGKYHDMEES
                     PQPAAYLPLSQSEQSEAAFVVRSQRPPGEMAAALQRTLSGIQPNTPVAVLSWPDALSD
                     VLFPARAATVALGIMGLLAAMLAVTGIFGMANYSVSRRMKELGVRMALGARRRHVISA
                     AVGRPMVLLGLGSLLGLLTGVLTSRLLQQIVYQANPGDPAIVGGAVLTMALLGAAASA
                     IPALRALAVDPSELMRED"
BASE COUNT          592 a          885 c          955 g          574 t
ORIGIN      
        1 cagatagtcg tgggcggcga agaggccggt cttagtggcg tttcttgagg agaccgcttt
       61 accggattcg gtgcgggggc ctgtggatgt gggaaaagat gtttctttca tgccttagcg
      121 taggcatatt gtgtcaagca aaccggggtc gatttcgggt aagtctatga aagcattcgt
      181 taaaaaaggg gtgcgaaacc tgtgacgact cgctgtacga gtcagttctg cttagtcttc
      241 gcgcatgagt tcggagggat cgacggcaag tgctcgcaat gctggaatgg cggaggccgc
      301 ggcgcccagc agggccatcg tgagcacagc gccacccacg atcgccgggt cgcctggatt
      361 ggcctggtat acgatctgct ggagcaagcg gctggtgagg acaccggtga gcagacccag
      421 cagcgatccg aggccgagca gcaccattgg tcgtcccacc gccgcgctta ttacgtgtct
      481 cctgcgcgcg ccgagggcca tgcgaacgcc cagttccttc atccgccgac tcacgctgta
      541 gttcgccatg ccaaagatgc ctgtcacggc aagcatcgcg gcgagcagcc ccatgatacc
      601 cagcgccaca gtcgcagcgc gtgcggggaa cagcacatcg gacagcgcat cgggccagct
      661 taggaccgca accggcgtat tcggctggat accgctgaga gtgcgctgaa gcgccgccgc
      721 catttcgccc ggcggacgtt gcgaacgcac cacgaacgcc gcttcgctct gctcgctttg
      781 cgatagcggc aggtaggcgg cgggctgggg cgactcttcc atgtcatggt atttgccggt
      841 ctccgccacg cccactactt cggtgagatt gccggacacg acaaagtatt tcccgatggg
      901 cggcgcttcg ccccacattc tgcgcgcgaa cgattggttc acgatgccga cgcgcggcga
      961 atccgcggta tcgtgccatg agaaatcgcg cccggtaagc agccgggtac cggcagcttc
     1021 gaggtatccc ggcgacatct gaaagacgta cgatgacagt gcggaattgt ttagcttgaa
     1081 gtcgtttacg cccgcccgga aaatcggaat gccatgaatt cctccggtca tgggggtacg
     1141 gttgaccatg cccgccgacg tgacgcccgg gatggcgcgc gccgcttcaa taaccggctt
     1201 ctttttatcg gccgggatgt cgcctaattc gacgagcatc gcgccgcgcg gctggaagcc
     1261 aaggggggcg cgcagtacac ggaccatgcc gcgcacggcc acaagcgagg ccgttatcag
     1321 caaggtgcag atcgcaattt gcgcgcccag caacagatct ctcatggcaa cgtgacggag
     1381 gcgaggggcg ccgggcgggc cgtttttcat catgcgcagc ggagtgccgc gccccagctg
     1441 gcgcacaggc gccattccaa ataacagggc gctggccagc gtgaatatca gaccggccag
     1501 gtacacacgc gcatccacgt ttaccgttag cgcgccagcg aatggctgcg accgattcag
     1561 caagcgcagc agcagctgcg cgcccacaac gcccgcggcg cctcccatca gcgacacgat
     1621 aaccgcttcg gtgagcaact gtcgcacgag gcgcagacgg ctggacccga gggccacacg
     1681 gagcgccagt tccctgcccc ggtccgcggc gcgcgcggca aacagagttg cgaggttcgc
     1741 acatgccgcc gtcagaacca gcaaggccag tgcggtgacg ctccagagga atccgcgaat
     1801 gacgtcaccg ttatcgccgt agaggcctgg atggataagc cggagcggct gtccgtcgtc
     1861 ggtctccgga taaatttttg cgagcccggc cgaaattgca ttcagattgt cggtcgcctg
     1921 ttgcggcgtg actccgggct tgagccggcc aagcactgta acggttacgg ccgtccggct
     1981 atgcaggtaa tcactcttat cgatttgcgc ctggttcgcc attggaatcc agtaatccgg
     2041 ccacacaaat cgctccgcgc cgtggaaccc ggggggcgcc acgccgatca cggtgaaggg
     2101 atgtttgtta aggtccacgg ttgcgccgac tacacccggg tcggattgaa acacgctgcg
     2161 ccacagagta tcgctcaaga ccacatacgg ggctgaaccc ggaccattct cgtcaccgtc
     2221 gcggaacaat cggcctgccc gcgggtacac cccgagcaga tcgaaatagt ttccggtgac
     2281 ctcatcgccg tggaccttca gcgcggtgtt atgccagctg agcgtcgcat ttgaatatcc
     2341 ataaacgccg gccagcccgc tgaacgtggt gttttgctgt cgaaaatctt ccaatgcggg
     2401 atatgacgtg gtgaggagtc tgccgctcat ccaccgctta tgacgaagct gatagaggct
     2461 ttgcggatcc tttacaggga gaggctgaag caggactgcg ttcacgactc cgaagacgac
     2521 gacgtttgct ccgatgccga gcatcagggt gattagggct acgaccgtaa acgcggggga
     2581 ttttcgaagc acgcgaagag aataacggat gtccaggagc aggttgtcca cgaatggcag
     2641 gcccttttcg gcgttgtagt cgtgcgtaag ggcggcgacc gctccgaatt taaggcaggc
     2701 ttgacggcgg gcttcgtggt gggccatgcc ggcgcgaata ttctcgtcgg tttgggcggc
     2761 tatgtgggat tcaatttctt cccgcagccg ttcgtcgccg cggcggttga cagtgaagtt
     2821 gagcaagcgt gcgagcgagc gacgcagggt tctcatgcaa gatcgtccgc tttcacttca
     2881 aggaagcgtg caatgatgga cgctgtttgc tcccaacccc gtttttcggc ttcgagtgat
     2941 ttgcggcctg tgcgggtgag gcgataaaac cgggcgcggc gattattttc ggaaggcccc
     3001 caatcg
//
LOCUS       DATIMH010000107         1865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2914689, whole genome shotgun sequence.
ACCESSION   DATIMH010000107 DATIMH010000000
VERSION     DATIMH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2914689"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1865
                     /locus_tag="VLI90_01820"
     CDS             <1..>1865
                     /locus_tag="VLI90_01820"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV12969.1"
                     /translation="TPTPGAITGIVYNDQNGDGIRQAGEPGLSGWTVFIDQNANSTLD
                     AGEPSTVTDASGVYTLSNLAVGANQLTEIPQVGFSATAPGTGTQLVSVPNGTTVAGIN
                     FGNKQRTDAGIGGTAFDDVNHNGVRDLGERGLAGLSVYLDLNNNGALDAGEPSLVTSA
                     DQFFTPAVDEAGTYLFTHLPAGTYTVRETLPVELSATPAAVATQTITLAPGELRSNVN
                     FADVFRAGEIHGAKFDDLNGNRRQDPGEPPMAGVTVYLDLNRNDQLDAGEPSTVTAAD
                     GSYAFTGLTPGAYVVRELLPHGHEHTYPATLPGGILWPDGVSNPAVGNVSPTSITTSL
                     AEGETFSQPVSLTLPAGGALTNKADVFLLFDDTGSFTGNSPIVRAAFPQIINSLQTAL
                     PGLDLGFGVGRFEEYGNFAAEFATGRPFILNQSIVSQTTPGFSTSIQSALDRTTPGYG
                     GDQPETDIEALFQMVTGAGFDGNNNGTTTDSGAAGLVSTQLAPGGSGDVPSFASYTAD
                     ATGNVLPAEGNIGGAGFRAEALPIILLATDTGFAYQPKGETSITGLGGLTLPISQLTQ
                     TSRPTTPFNSGAGIQETITGLNALGALVIGLGTNSQTNIDPRQQLESIARLTGAL"
BASE COUNT          341 a          618 c          619 g          287 t
ORIGIN      
        1 tcacaccgac gccgggggcg atcacgggca tcgtgtacaa cgaccagaac ggcgacggca
       61 ttcggcaggc gggtgaaccg gggttgtccg ggtggacggt gttcatcgat cagaacgcca
      121 acagcacgct ggacgccggc gaaccatcga cggtgacgga cgccagcggc gtctacacgc
      181 tgagcaacct cgccgtcggc gccaatcagc tgacggaaat tccgcaagtg ggtttcagcg
      241 cgaccgcgcc cggcacgggc acgcagttgg ttagcgtgcc caacggaacc accgtggcag
      301 gcatcaactt cggcaataag caacgaaccg atgcgggcat cggcgggacc gcgttcgatg
      361 acgtgaacca caatggcgtg cgcgacctcg gcgagcgcgg actcgcgggc ctgtcggtct
      421 atctcgacct gaacaacaac ggcgcgctcg acgcgggcga gccgtcgctg gtgacatccg
      481 ccgatcaatt cttcacgccc gcggtcgatg aagcgggcac ttacctgttc acgcatctgc
      541 cggccgggac gtataccgtt cgtgagaccc tgcccgtgga gctgagcgcg acgccggcgg
      601 ccgttgcgac acagacgatc acgcttgccc cgggcgagct gcgatcgaac gtcaactttg
      661 ccgacgtttt ccgagccggc gaaatccacg gcgcgaagtt cgacgacctg aacggcaatc
      721 gccggcagga cccgggcgaa ccgccgatgg cgggcgtgac ggtgtatctc gatctgaacc
      781 gcaatgacca attggacgcc ggtgagccga gcaccgtgac ggcggcggat ggatcgtacg
      841 cgttcaccgg cctgacgccc ggcgcgtacg tcgtgcgcga actgcttccg cacgggcacg
      901 agcacacata ccccgccacc ttgcccggcg gcatcctttg gcccgatggt gtgagcaacc
      961 ccgccgtcgg caatgtgtca ccgaccagca tcaccacctc gctggccgag ggtgagacgt
     1021 tcagccagcc ggtgagcctc acgttgcccg cggggggcgc gctgacgaac aaggcggacg
     1081 ttttcctgct cttcgatgac accggcagct tcaccggcaa cagcccaatc gttcgggcgg
     1141 cgttcccgca gatcatcaat tcgcttcaga ccgctctgcc gggcttggac ctgggctttg
     1201 gcgtgggacg gtttgaagag tacgggaatt ttgcggccga gtttgcgacg ggtcgcccgt
     1261 tcatcctgaa ccaatcgatc gtgtcgcaga cgacgccggg gttttcgacg tcgatccagt
     1321 cggcgctgga ccgcacgacg ccgggctacg gcggcgatca gccggagacc gacatcgaag
     1381 cgctcttcca gatggtcacc ggcgccggtt tcgacgggaa caacaacggc acgaccaccg
     1441 acagcggcgc ggcaggcctg gtctccacgc aattggcgcc aggcggcagc ggcgacgtgc
     1501 cgtcgttcgc atcgtacacc gccgatgcca ccggcaacgt gctgccggcc gagggcaata
     1561 tcggtggcgc cggcttccgc gccgaggcgc tgccgatcat cctgctggcg accgacacgg
     1621 gcttcgcgta tcagcccaag ggcgagacat caataaccgg cctgggcgga ctgacgctgc
     1681 cgatttcgca gctgacgcaa acctcgcggc cgacgacgcc gttcaattcc ggcgcgggca
     1741 ttcaggagac gatcacgggg ctgaacgcgc tgggcgcgct ggtgatcggg ctggggacga
     1801 attcgcagac gaacatcgac ccgcggcagc agcttgagtc gatcgcccgg ctgacgggcg
     1861 cgctc
//
LOCUS       DATIMH010000108         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3780320, whole genome shotgun sequence.
ACCESSION   DATIMH010000108 DATIMH010000000
VERSION     DATIMH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3780320"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VLI90_01825"
     CDS             complement(<1..772)
                     /locus_tag="VLI90_01825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSV12970.1"
                     /translation="MPQGRSQFVKPRLPSLDGLRAVSIALVLLQHASLTPSFPFALAP
                     LGDAGFGVHIFFVISGFIITLLLLDEERENDGISLKRFYLRRMVRILPVFWTFLLIML
                     LARRIYVGEFFQAATFTAGMGLWSTPGTWNTAHGWSLAVEEQFYLLWPAAMVLLGRTS
                     WRVALLIAVVALANFTRCWAMTHSAVYWSMSHPGHWWSPFERAWQLMGVRTFLSHVDL
                     LACGCLLAIAMQRRPSLVQTIANWRPAIGRPRAVIVMYL"
     gene            complement(788..1102)
                     /locus_tag="VLI90_01830"
     CDS             complement(788..1102)
                     /locus_tag="VLI90_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12971.1"
                     /translation="MSCSNSLDLRNAIREILLADWDPSNAVRFEAARGEYDGYLSPLA
                     DLIKRGSSEDDLIDFLYAREREIMCFPGLGKQRLRRVARKLLALRARRALRARRDGTV
                     TR"
     gene            complement(1799..2389)
                     /locus_tag="VLI90_01835"
     CDS             complement(1799..2389)
                     /locus_tag="VLI90_01835"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV12972.1"
                     /translation="MDWQAYDALLHAVGDHHVFVTYDRGRVELMYPSFEHENRSEIIG
                     LLIRSIAEGLTMKVKGGGSTTFRREDIDRGLEPDKCFYVANEAKVRGKTNIDLSLDPP
                     PDLCVEVEISRRLLNRPEIYASLGVPELWRDDGKHLRIFALSPHADYRQCDRSITFPE
                     LSADQLNELLDASHVTDETSWATDVRAYARKLRQQP"
     gene            complement(2443..>3337)
                     /locus_tag="VLI90_01840"
     CDS             complement(2443..>3337)
                     /locus_tag="VLI90_01840"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSV12973.1"
                     /translation="DACKARKLPIRVGVNEGSIIERRDKQKRLKELGGIFSNEKHGHL
                     LAIMITKLEEYLDIFYERDFKDIVISAKSMDAALTIDAYTEISRRFDHPLHLGVTHAG
                     PKETGTIRSVVPLGHLLASGIGDTVRISYANDPIYEVQDGLELMYILGLRTRIGAELI
                     ACPTCGRIQVDLFKMVQDGRKRLEKEISLPIKVAVMGCIVNGPGEAEGADVAIFAGDR
                     RGIIYVQGEKVANVPEEQILPALLDECRKFQDRVQRGEAKLGEKKVDILPPDPIGELG
                     SGWEKIQKEKSEKGLVLPVLK"
BASE COUNT          638 a         1077 c          981 g          641 t
ORIGIN      
        1 ctaaatacat cacgatgacg gcgcgcggcc ggccgatcgc cggccgccaa tttgcgatcg
       61 tctggacgag acttggtctg cgctgcatgg cgatcgccag caggcaaccg caggccagca
      121 ggtccacgtg cgatagaaat gtgcgcacgc ccatcagctg ccacgcgcgt tcgaatggtg
      181 accaccaatg gccgggatgg ctcatcgacc agtagaccgc cgaatgcgtc atcgcccagc
      241 atcgcgtgaa attcgccaag gcaacgacgg cgatcagcag cgcgacgcgc caagaggtgc
      301 ggccgagcaa caccatcgcc gcgggccaga gtagatagaa ctgttcctcg acggccagcg
      361 accaaccgtg agccgtgttc cacgtgcccg gcgttgacca cagccccatc cccgcggtga
      421 acgtcgcggc ttgaaagaac tcgccgacgt agatccgtcg cgccagcagc atgatcagca
      481 gaaacgtcca aaacaccggc agaatacgaa ccatgcgacg aaggtaaaag cgcttcagcg
      541 agatgccatc gttctcgcgc tcctcgtcca gcagcagcag ggtgatgatg aagccgctga
      601 tgacgaagaa gatgtgcacg ccgaacccgg cgtcgcccaa gggcgccagc gcgaacggaa
      661 agcttggcgt cagcgacgcg tgctggagga gcacgagcgc aatcgagacg gcacgaaggc
      721 cgtccagcga gggcagccgc ggcttcacga attggctgcg gccctgcggc atactcacga
      781 tccgaagcta ccgagtgacg gtgccgtcgc gccgcgcgcg cagcgcgcgc cgcgcgcgca
      841 gcgcgagcag cttccgcgcg acacgtcgca ggcgctgctt gcccaagcct gggaagcaca
      901 tgatctcgcg ctcgcgcgcg tagagaaagt cgatgagatc gtcttctgaa gagccgcgct
      961 tgatcaggtc cgccagcggc gacaaatagc catcgtactc cccgcgcgcc gcctcgaacc
     1021 ggacggcatt gctggggtcc cagtcggcga gaaggatctc gcgaatggcg ttgcgaagat
     1081 cgagtgaatt gctgcacgac atcaccgcac aaccggcagc acgagccgct ttatggcctt
     1141 ttcagctggc taattctagc caagattacc ctttagcgcc gagtagtcgc cgtagccaag
     1201 aacggcggcc gctgaaagct ctagctccac ttgcgggata gcttaataca accggggcta
     1261 atgccgctga tgcggtgctt actccgatcg actcacaggc gacggtctta acgtgcgctg
     1321 gattggtttt cagttgcgta atgactttcc acggctgtcc atcgatgtca tccaggagtg
     1381 caaccagcaa atgactcgac gatatcgttc gatgaccgcg gcagcggccg gagcgcattg
     1441 ccctggcaat ggcccttctc gcatcgctgt ttagcggcag ctgtcccgag atgactgcgt
     1501 cttgtccacg cgggactggt tcgcaaagcc ggcgcagctc ccacaacgag attccgaagc
     1561 gcgacaaaac tcgcgcgccg ttaccggttc cttcgcgcgc gattcccagc aacagcgaat
     1621 acggtgtgat tgcagccaca ccctgagcgg ccgactcttc gctcgccaat ctaaccgccg
     1681 cagccgcatc ggcgtctaat cgtcgccaga agctcattgg ttctgctcgt caaatgctac
     1741 ctcgccgccg gccgcaccag ccgtttttcg ccttttcgac cgtgcgctcc attcagcgct
     1801 aaggttgttg ccgcaacttt cgggcgtagg cgcggacatc cgtcgcccaa gacgtttcgt
     1861 ctgttacgtg tgacgcatcg agcaactcgt tcaattgatc tgcggacaat tccggaaatg
     1921 tgatgcttcg atcgcactgg cgataatcgg cgtgtggcga gagggcgaag atgcggagat
     1981 gcttgccatc gtcgcgccac agttccggca cacccaggct cgcgtaaatc tctggccgat
     2041 tcagcagccg tcggctgatc tcgacctcaa cgcataggtc cggcggtggg tccagcgaaa
     2101 gatcaatgtt cgtcttgccg cgcacctttg cttcattcgc cacgtaaaaa catttgtccg
     2161 gctccagccc gcggtcgatg tcctcgcgcc ggaaggttgt tgagcccccg cccttcacct
     2221 tcatcgtcag tccctcggcg atggaacgga ttagcagtcc aatgatctcc gatcggttct
     2281 cgtgttcgaa tgatgggtac atcaactcga cccttccccg atcgtacgtg acgaagacgt
     2341 gatgatcgcc gacggcgtgc agcagcgcgt cgtacgcttg ccagtccatg ccgtcgagta
     2401 cgaatcgccg atcggcaggg tcaagagtag ccgtcgtcgc gctcatttta acaccggcag
     2461 cacgagcccc ttttccgact tttccttctg gatcttttcc cagccgctgc ccagttcacc
     2521 gatcgggtcc ggcggcagga tgtccacctt tttctcgccc agcttcgctt cgccacgttg
     2581 cacacggtct tggaacttgc ggcactcgtc cagcagcgcc ggcaggatct gctcctccgg
     2641 cacgttcgcg accttctcgc cctgaacgta aatgatcccg cggcggtcgc cggcgaagat
     2701 ggcgacgtcg gcgccttcgg cttcgcccgg gccgttcacg atgcagccca tgacggccac
     2761 cttgatcggc aggctgatct ccttctccag ccgcttgcga ccgtcctgca ccatcttaaa
     2821 cagatcgacc tgaatccgcc cgcaggtggg acaggcgatc agttccgcgc cgatgcgcgt
     2881 gcgcagcccg aggatgtaca tcagctcgag gccatcctgc acttcataga tcggatcgtt
     2941 ggcgtagctg atgcggacgg tgtcgccaat cccgctggcc agcaggtggc ccagcggaac
     3001 aacggaccga atcgtccccg tctccttcgg ccccgcgtgc gtgacgccca ggtgcagcgg
     3061 gtggtcgaag cggcgggaga tctccgtgta cgcgtcgatg gtcagggccg cgtccatgct
     3121 cttggcagaa atcacgatgt ccttgaagtc ccgctcgtag aagatgtcca ggtattcctc
     3181 gagcttggtg atcatgatgg cgaggaggtg gccgtgcttt tcatttgaaa agatgccgcc
     3241 cagctccttg aggcgtttct gcttgtcgcg ccgctcgatg atcgagccct cgttgacgcc
     3301 cacgcggatc gggagttttc tcgccttgca cgcgtcg
//
LOCUS       DATIMH010000109         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2915011, whole genome shotgun sequence.
ACCESSION   DATIMH010000109 DATIMH010000000
VERSION     DATIMH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2915011"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VLI90_01845"
     CDS             complement(<1..455)
                     /locus_tag="VLI90_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12974.1"
                     /translation="MVPLPIDSSLPDIVQRLREARSLVLVAPPGAGKTTRVPPAILRA
                     NLLSPEHPNLVMLQPRRVAARTVAARIADENRWRLGDEVGYHVRFDRMLSNHTRLRVL
                     TEGILTRQLLDDPYIEGIGAVLLDEFHERSLYTDVAVALLREVQQTVRP"
     gene            882..2234
                     /locus_tag="VLI90_01850"
     CDS             882..2234
                     /locus_tag="VLI90_01850"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSV12975.1"
                     /translation="MRTIAIINQKGGCGKTTTSINLAACLARLGQKTLLVDMDPQGHC
                     GVGLAVPEDQIERTVLDALIEESDGKPAKVSEIVWQIASEFDLAPSNIKLAAFEQLFA
                     GRDGREDRLAKALAVVKNTYQWCIIDCPPSVGLITFNALKACDEVVVPVETGYFSLHG
                     LAKMMETLEVMKDRCGRDILIRVLPTLYDTRTKLAREVLSELRTKFRDYLMESTVNFN
                     TKLKEAASFGQPITEYDPGSRGYKDFVNLARELMGHRPVDLEPTPTETLSRPAELVQR
                     AKQLAQLANFQFGRGTVPAPTAPAAQPTEVKPMKTAGPATVSRASEMGLDDLDHSINS
                     HGGGIATLAAPAKAKTTAQKIEEFYGVKQVGDEVMFAARFDNAKKVLIAGDFNNWSPM
                     STPMITAGAPGVWTSRLPLQPGRYRYRFVVDGKWMTDPNNSYVETNQFGELNNVVEVD
                     "
     gene            2322..3845
                     /locus_tag="VLI90_01855"
     CDS             2322..3845
                     /locus_tag="VLI90_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12976.1"
                     /translation="MSDEQQKKKSLSEISHLFLSSVRDNATGGVRPKRLPPGAPRPDV
                     SVDLTPEEFAQVYGGAASVEPERPAIAPVSAIIGAHLNGKQFDRVKEYARHLAAGDKR
                     IGLIEVDASEFRVMCFDRGTADMNDQHDEPVETYDARQMSESLDELAWDIDHWLLLLP
                     NPRTPEARALLRDVGHWVLLSTCDHDGVVSCYRAIKGLLDSTHRPRLTLALLNPADQT
                     EAGRVYRKIAGVCEQFLSWPMEAEPAVQPADDVAEHLVLLCRPNREKSAVAIAPQWQI
                     VADFLTRARADAAARRRASAGSSHPSHETSDDAGAEMEIEAEPMPAPAQHLADVVIPA
                     EPVAAPVIAPAPAAPPAPVTAAAAASVDADDSEITDLPAGETTEAAVLAAVLKKQNLG
                     LIECPVRPPTCPNAILAVNRDRRLVILAVTRQGLAELRSIGQAYRWVIENRPLLSMAL
                     PQFAIDAHAMPCLRLLVDRADLSADLLQPMLESGNVTVQTYRKLKWGGRTGLLLEAA"
BASE COUNT          780 a         1254 c         1246 g          635 t
ORIGIN      
        1 tccggccgaa cggtctgctg cacttcgcgc agcagcgcga cggccacgtc ggtgtacaag
       61 cttcgctcgt ggaattcatc cagcagcacc gcgccgatgc cctcgatata tggatcgtcc
      121 agcaactgcc gtgtcaggat gccttcagtc agcacgcgca gccgcgtgtg gttcgacagc
      181 atgcggtcga agcggacgtg atagccgact tcatcgccca gtcgccagcg gttctcgtcg
      241 gcgatgcgag ccgcgacggt gcgagccgcc acccgccgcg gttgcagcat gacgaggttc
      301 gggtgctcgg gcgacagcag gttggcccgc aaaatcgccg gcggcacgcg cgtcgtcttg
      361 cccgcgcccg gcggggcgac cagcaccagc gaccgcgcct cgcgcaggcg ctggacgatg
      421 tccggcaacg atgaatcgat cggcagcgga accattcgtg gcggattgta cggcgtcgct
      481 ccgccgttgc ggagcaggag caacgcaaat gaccaatgac caaaacccag atcccaatga
      541 aatcccgatg agcccaacga ccaatggacc aattgggatt tggtcattgg cattcattgg
      601 gatttggcat ttgaacattg gtcattcaag aaactgaggc gcgtggaatt gtccgcagaa
      661 tccccaagac cccgcctatc agcactgata ttcgcctcgc ccgcggctga aaattcccca
      721 gcttgacccc ataatcctaa agttttcagt ccccgtcgcc cgattcgttt attggaacag
      781 agttgggagc gggcacttcc aacgtcgaac cagcaacaaa atcccaacgc gcggccgacg
      841 gattggcctc atgcgcgcca gcgagccaag gaaggtctcc gatgcgaacg atcgccatca
      901 ttaatcaaaa gggcggatgc gggaagacaa ccaccagcat caacctggcc gcgtgcctcg
      961 cacgcctggg gcagaagacg ctgctggtgg atatggatcc gcaaggtcac tgcggcgtcg
     1021 ggctggcggt gccggaagat cagatcgagc gcaccgtgct ggacgcgctg atcgaagaat
     1081 ccgacggcaa gcctgccaag gtcagcgaga tcgtctggca gatcgccagc gaattcgatc
     1141 tcgcgccaag caacatcaag ctggccgcgt tcgagcagct gttcgccggg cgcgacggcc
     1201 gtgaagaccg gctggccaag gcgctggctg ttgtgaaaaa cacttatcag tggtgcatca
     1261 tcgactgccc gccgagcgtg gggctcatca cgttcaacgc gctgaaggcg tgcgacgaag
     1321 tcgtcgtgcc ggtggagacc ggttacttca gcctgcacgg cctggcgaag atgatggaaa
     1381 cgctggaggt catgaaggac cgctgcgggc gcgacattct catccgcgtg ctgccgacgc
     1441 tttatgacac gcgcaccaag ctggcccgcg aggtgctcag cgaactgcgg acgaagttcc
     1501 gcgattacct gatggaatcg accgtcaact tcaacacgaa gttgaaggaa gccgccagtt
     1561 ttggacagcc gatcaccgaa tacgatccgg gcagccgggg gtacaaggat ttcgtgaacc
     1621 tcgcccgcga gttgatgggc catcgcccgg tcgatctcga accgacgccg acggaaacgc
     1681 tgtcgcggcc agcagagctg gtgcagcgcg ccaagcaact ggcgcagctg gcgaacttcc
     1741 agttcggccg cggcaccgtg ccggcgccga ccgcgcctgc cgcgcagccg accgaagtga
     1801 agccgatgaa gacggccggc cccgcaacgg tttcgcgcgc cagcgagatg ggtctggacg
     1861 atctcgatca ctcgatcaac agccacggcg gcggcatcgc caccctggcg gccccggcca
     1921 aggccaagac gaccgcgcag aagatcgaag agttttacgg cgtgaagcag gtgggcgatg
     1981 aagtgatgtt cgccgcccgg ttcgacaatg cgaagaaggt gctgatcgcc ggcgacttca
     2041 acaactggtc gccgatgtcc acgccgatga tcaccgccgg cgcgcccggt gtgtggacca
     2101 gccgcctgcc gctgcaaccg gggcgatatc gctatcgctt cgtcgtcgat ggcaagtgga
     2161 tgaccgatcc gaacaacagc tacgtcgaga ccaatcagtt cggcgaattg aacaacgtcg
     2221 tggaagtgga ttgatagcgt cagtggtcag ttgtccgttg tcagttgaaa atcctggcaa
     2281 caaccaactg acaacggaca actgacaacg gacgaattcg catgtctgac gaacagcaaa
     2341 agaagaagag cctcagcgag atcagccacc tgttcctcag cagcgtgcgc gacaacgcga
     2401 ccggcggtgt gcggcccaag cgtctgccgc ccggcgcgcc gcggccggat gtcagcgtcg
     2461 atctgacacc ggaagaattc gcacaagtct acggcggcgc cgcctcggtt gagccggagc
     2521 gtcccgccat cgcgccggtc tccgccatca tcggcgccca tctaaacggc aagcagtttg
     2581 accgcgtgaa ggaatacgcc cgccatctgg cggcgggtga taagcgcatc ggcttgatcg
     2641 aagttgacgc cagcgaattc cgcgtgatgt gtttcgatcg cggcaccgct gacatgaacg
     2701 atcagcacga tgagccggtc gaaacgtatg acgcccggca gatgagcgag tcgctggacg
     2761 agttggcgtg ggacatcgat cattggctgc tgctgctgcc gaacccgcgg acgcctgaag
     2821 cccgcgcgct gctgcgcgat gttggccact gggtgctgct cagcacctgc gatcacgacg
     2881 gcgtcgtctc ctgctatcgc gcgatcaagg gcctgctcga ttcaacccat cgtccgcggc
     2941 tgacgctcgc gctgctgaac ccggccgatc agacggaagc gggtcgggtg tatcgaaaga
     3001 tcgcaggcgt gtgcgagcag ttcctcagct ggcccatgga agccgagccc gcggtgcaac
     3061 cggcggacga cgtggccgag cacctggtgc tgctgtgccg gcccaaccgc gaaaaatccg
     3121 cggtggcaat cgcgccgcag tggcagatcg tcgcggattt cctgacccgc gcccgagccg
     3181 atgccgccgc ccgacgacgg gcctcggcag ggtcgtcgca tccatcgcat gaaacatcgg
     3241 acgatgcggg agccgagatg gaaatcgaag cagaaccgat gccggcgccg gcgcagcatc
     3301 tggctgatgt cgtcattccc gccgaacccg tcgcggcgcc cgtcatcgcg ccagcgcctg
     3361 cggcgccgcc cgcgccggtc acggccgccg cggcggcgtc agtcgatgcc gacgacagcg
     3421 agatcaccga tctcccggcc ggcgagacga ccgaagccgc ggtgctcgcg gccgtcttga
     3481 agaaacaaaa tctgggtctg atcgaatgcc cggtgcggcc gccgacgtgc ccgaatgcga
     3541 tcctcgcggt gaaccgcgat cggcgcctag tcatcctcgc cgtcacccgg caggggttgg
     3601 ccgagctgcg ttcgatcggt caggcctatc gctgggtcat cgaaaatcgc ccgctgctgt
     3661 cgatggcgct cccgcagttc gcgatcgacg cccacgcgat gccgtgcctt cgcctgctgg
     3721 tcgatcgcgc cgatctcagt gccgacctgc tgcagccgat gctcgaaagc ggcaatgtca
     3781 cggtgcagac ctatcgcaag ctgaaatggg gcggccgcac cggcctgctg ctggaagcgg
     3841 cgtaacgcac cactcacgcc gggttcggag cagcctgccc tgagcctgtc gaagggtact
     3901 cgcgacgacc cggat
//
LOCUS       DATIMH010000110         1958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1760061, whole genome shotgun sequence.
ACCESSION   DATIMH010000110 DATIMH010000000
VERSION     DATIMH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760061"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..858)
                     /locus_tag="VLI90_01860"
     CDS             complement(13..858)
                     /locus_tag="VLI90_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSV12977.1"
                     /translation="MTLGTYGWLLRQSLLSSLDDGCFGYAKAAAYSALLSFFPVLTSS
                     AAILVQTRAEFVSRTLQNFLSQIVPPGTEDLVVQQFRVMGERPVGFLVAAGAISLWAA
                     SGVIKSLIEGFQAAYRVPRNRDFIRQSGVAMSLVLLSAVPLICASLLIVFGGQVERIV
                     LNWVKVDPFLTPFAWVWQAASRLARYVLAFVTTATVTSSLYYFGPNRKQRWKYVWRGA
                     WLATFLWLFATLGFAWYVRHLARYNVMYGSIGAGIALLVWMYMIALIALIGCEFNASY
                     ERTAG"
     gene            complement(890..>1958)
                     /locus_tag="VLI90_01865"
     CDS             complement(890..>1958)
                     /locus_tag="VLI90_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV12978.1"
                     /translation="TASQALSGKLYDRVGNRRGFSISIIVWSVASMLHAFATGLFSLN
                     CFRFLLGLGEAGNWPGAAKVIAEWFPIRQRALGMAIFNSGTSIGSVIAPPLIIALQLA
                     YGWQATFLAVGSLGFVWLILWLSFYHPPAEHPRITAAELALIREDRTPAAAPLSWAQL
                     LRYRETWAIVLSRFCCDPVWWLYITWLPLYLYKVHGFSLKEIGLFAWVPYVAADAGSL
                     FGGWMSGHLIARGWTANRARKTVIVAGMLCMCTGLLAAVAHTAAVALAAIAVVLFGFQ
                     TWINNVQTLPSDYFPENAVGSVAGLGGMGAGIGAILFTQATGFVVDHFSYTPMLVIAG
                     LLPLLGTALLFVLGGRIRRVA"
BASE COUNT          439 a          644 c          606 g          269 t
ORIGIN      
        1 gcggaaaata ccctaccccg ccgtacgctc gtagctcgca ttgaactcgc agccgatcag
       61 cgcgatcagc gcaatcatat acatccacac cagcagcgcg atcccggcgc cgatcgagcc
      121 gtacatcacg ttgtatctgg cgaggtgccg cacataccag gcgaacccca gcgtggcgaa
      181 caaccacagg aaagtggcca gccatgcgcc gcgccacaca tacttccagc gctgcttgcg
      241 attaggtccg aaatagtaaa gcgaagacgt caccgtggcc gtcgtcacga acgcgagcac
      301 atagcgcgcc aaccggctgg ccgcctgcca cacccacgcg aagggcgtca ggaacgggtc
      361 gaccttcacc caattcaaga cgatacgctc cacctggccc ccgaatacaa tcagcagaga
      421 tgcgcaaatc aatggcacgg cagagagcag caccagcgac atcgcgaccc cgctctggcg
      481 tatgaaatcg cgattgcgcg gcacgcggta tgcggcctga aagccctcga tcaagctctt
      541 gatcaccccc gacgctgccc acaacgaaat cgcgccggcg gctaccagaa atccgaccgg
      601 ccgctcgccc atcacgcgga actgctgcac caccaggtcc tcggttcccg gaggcacaat
      661 ctgggagaga aaattctgca gcgtcctgga gacgaattcg gcgcgcgtct gcaccaggat
      721 ggcggcggaa ctcgtgagca cggggaagaa gctgagcagc gcggaataag cggcggcctt
      781 ggcatatccg aagcagccgt cgtccaggct ggagagcagg ctctggcgga gcagccagcc
      841 ataggtcccg agagtcatcc gcactagggt cagtatagcg gaggcattat cacgcgacgc
      901 gccgaatgcg gccgccgagc acgaacaaca gcgcggtgcc gagcagcggc agcaggcccg
      961 cgatcaccag catcggcgtg tacgagaaat gatccaccac gaagcccgtg gcctgcgtga
     1021 acagaatcgc gccgataccc gcgcccatac cgccgagccc cgcgacggag ccgacggcgt
     1081 tctcagggaa gtaatcgctc ggcagcgtct gcacgttatt gatccaggtc tggaagccga
     1141 agagcaccac cgcgattgcc gcaagcgcta cggcggcggt gtgcgcgacc gccgccagca
     1201 ggccggtaca catgcagagc attcccgcca cgatcaccgt tttgcgcgcg cggttcgccg
     1261 tccagccgcg cgcgatcagg tggccggaca tccagccgcc gaagaggctc cctgcgtcgg
     1321 cggcgacgta aggaacccac gcgaagagtc cgatttcctt gaggctgaag ccgtgcacct
     1381 tgtagagata gagcggcagc cacgtgatgt acagccacca caccgggtcg cagcagaagc
     1441 gcgagagcac gatcgcccac gtctcccgat agcggaggag ttgcgcccag gagagcggcg
     1501 ccgctgccgg tgtacggtcc tcgcggatca gcgcgagttc ggccgccgtg atgcgcgggt
     1561 gttcggcggg tggatgataa aacgaaagcc acaggatcag ccatacgaag cccagcgatc
     1621 cgacggccag aaaggtggcc tgccagccgt aagcgagttg cagcgcgatg atcagcggcg
     1681 gcgcgattac ggaaccgatg gaggttccgc tattgaagat cgccatcccg agcgcgcgct
     1741 ggcgtatagg aaaccactcg gcaatcacct tggcggcgcc gggccagttg cccgcctcgc
     1801 ccaatccgag caggaagcgg aagcaattca ggctgaagag ccccgtggca aacgcgtgca
     1861 gcatactcgc gacggaccac acgataatcg aaatactgaa cccgcggcga ttgcccacgc
     1921 ggtcgtagag tttgcccgag agcgcctggc tcgcggta
//
LOCUS       DATIMH010000111         3210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4649279, whole genome shotgun sequence.
ACCESSION   DATIMH010000111 DATIMH010000000
VERSION     DATIMH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4649279"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /gene="kdpB"
                     /locus_tag="VLI90_01870"
     CDS             complement(<1..1310)
                     /gene="kdpB"
                     /locus_tag="VLI90_01870"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HSV12979.1"
                     /translation="MQTITETKHAATPPSTRLGHPPAKSRKRNLWEAKIVRRALWDSV
                     GKLNPRNMMSNPVMFVVEVGSVITTVLLLMTPRQDFKFNLQITLWLWFTVLFANFAEA
                     MAEGRGKAQADTLRRARAETMANRLLPDGSIEQVPSSKLRADDTVLCSAGELIPGDGE
                     IVEGVASVDESAITGESAPVIRESGGDRSAVTGGTRVLSDQITILITSNPGETFLDRM
                     IALVEGAERQKTPNEIALNILLAGLTILFLLAVVTLQPFAIYSGAPQSIFVLVSLLVC
                     LIPTTIGGLLSAIGIAGMDRLVQHNVLAMSGRAVEAAGDVNTLLLDKTGTITLGNRQA
                     AQFIAAPNVTPAEMADAAQLSSLADETPEGRSIVVLGKERYGLRGRELAEHEAHFIPF
                     SAQTRMSGVNLDGYEIRKGAVDAIERYVHGNGYTMPAEVMAAVE"
     gene            complement(1319..3073)
                     /gene="kdpA"
                     /locus_tag="VLI90_01875"
     CDS             complement(1319..3073)
                     /gene="kdpA"
                     /locus_tag="VLI90_01875"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HSV12980.1"
                     /translation="MLALTKPLGVFMARVFERERTFMDPVLRPIERVIYRLTGVDEEH
                     EMRWTEYAVAMLLFSLVSMLLLYAMERLQTMLPFNPQKLTAVAQALAFNTAASFTTNT
                     NWQNYSGETTMSYFTQMAGLAYHNFVSAAVGIALAIAFIRGIARRQMQTIGNFWVDML
                     RACLWVLLPFCIVGALVLVSQGVVQNLRPYDTAKLLEPQQVQHVNANGKPAVDASGKP
                     VMDTVTSQTIAQGPVASQEFIKEFGTNGGGFFNANSAHPFENPTPVTNLIELVAIFAI
                     SSGLTYTLGRMTGSQAHGWAVWAAMAILFVAGVTVTYWAEAAGNPLFPAAVNQHATAS
                     QPGGNMEGKEVRFGITNTALWATVTTDASCGAINGWHDSFTPLGGMVPLVNMMLSEVI
                     FGGVGAGMYGMLIYIVLAVFIAGLMVGRTPEYLGKKIEAYDVTMAMLVALVFPLVILV
                     FAAISSVNGFGSSSILNPGPHGLTEILYSFTSQAGNNGSAFAGLNTNTPWYNVAGAFT
                     MLAGRFLMIIPMLAIAGNLAQKKYVPPSLGTFPVTTPLFTVLLIGTIVIVGALTFFPA
                     FSLGPVLEHLLMQAGKSF"
     gene            3006..>3210
                     /locus_tag="VLI90_01880"
     CDS             3006..>3210
                     /locus_tag="VLI90_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12981.1"
                     /translation="MNVRSRSKTRAMKTPSGFVSASTNAKNSRIWSHPLAVMSEPLGP
                     QERVHEVREQEHGNDDSRDEFERH"
BASE COUNT          632 a         1043 c          943 g          592 t
ORIGIN      
        1 tgctcgaccg cggccatcac ctccgccggc atggtgtacc cgttgccatg gacgtagcgc
       61 tcaatggcat ccaccgcgcc tttgcgaatc tcgtagccat ccaggttcac gcccgacatg
      121 cgggtttgcg ccgagaaagg aatgaaatgc gcttcgtgct ccgccaactc gcgcccgcgc
      181 agcccatacc gttctttccc cagcacgacg atcgagcggc cttccggtgt ctcgtccgcc
      241 agcgaagaga gttgtgcggc gtcggccatt tcagccggcg tcacgttggg cgcggcaatg
      301 aactgcgccg cttgtcggtt gcccagcgtg atcgttcccg tcttgtccag cagcagcgtg
      361 ttcacatcac cggccgcttc cacggcgcgt cctgacatgg ccaggacgtt gtgctgcacc
      421 aggcgatcca tgcccgcaat accaatcgcc gacagcaatc cgccaatcgt cgttggaatc
      481 aggcacacca gcaacgacac cagcacgaaa atcgattgcg gcgctcccga gtaaatcgcg
      541 aacggctgta gcgtgaccac cgccagcagg aagaggatcg tcaacccggc gagcaggata
      601 ttcagcgcaa tttcgttggg cgtcttctgc cgctcggcgc cttccaccag cgcgatcatg
      661 cgatcgagaa aagtctcgcc cgggtttgag gtgataagaa ttgtgatctg atcagacagc
      721 acgcgcgtgc caccggtcac agccgaacgg tcgccccccg attcgcggat cacgggcgcc
      781 gattctccgg tgatcgccga ctcatccacc gacgcgacgc cttcgacaat ttccccgtcg
      841 ccgggaatca gttctcccgc ggagcacaaa acggtgtcgt cggcgcgcag cttcgagctc
      901 ggcacctgct cgatggatcc atccggcagc aggcggttcg ccatggtctc ggcgcgggcg
      961 cggcgcagcg tgtctgcctg cgctttgccg cggccttcgg ccatcgcctc ggcgaaattc
     1021 gcgaacagca cggtaaacca cagccacagc gtgatctgta agttaaattt gaaatcctgc
     1081 cgtggcgtca tcaggagcag cacggtggtg atgacgctcc caacctcaac cacgaacatc
     1141 acgggattgc tcatcatgtt gcgcgggttg agcttcccca cggaatccca cagcgcgcgc
     1201 cggacgatct tcgcctccca caaattcctt ttgcgcgact tcgcgggtgg gtgacccagg
     1261 cgcgtgctcg gcggcgttgc agcgtgtttc gtttctgtga tggtttgcat gtgtgtctct
     1321 agaaggactt tcccgcctgc atcagcaggt gctcaagaac cggtcccagg ctgaaggcgg
     1381 ggaagaaggt cagcgcgccg acgatcacga tggtcccgat cagcagtacg gtgaacagcg
     1441 gcgtggttac cggaaaagtg cccagcgatg gcggaacata tttcttctgc gccaggttgc
     1501 cggcgattgc cagcatcggg atgatcatca ggaaacgccc ggccagcatg gtgaacgcgc
     1561 ccgccacgtt gtaccacggc gtgttggtat tcaggccggc gaaggctgag ccgttgttcc
     1621 ccgcttgcga ggtgaacgag tagagaatct cggttagccc atgcggcccg ggattcagaa
     1681 tgcttgagct cccgaaacca ttcaccgacg agattgccgc gaacaccaga atcaccagcg
     1741 ggaagaccag cgccaccagc atcgccatgg tcacgtcata cgcttcgatc ttcttgccca
     1801 ggtattccgg cgtgcgccct accatcagcc cggcgatgaa aaccgccagc acgatgtaga
     1861 tcagcatgcc gtacatcccc gcgccaacgc cgccgaagat gacctcactc agcatcatgt
     1921 tcacgagcgg caccatgccg ccgagtgggg tgaacgaatc atgccagccg ttgatcgcgc
     1981 cgcagctcgc gtcggtggtg acggtcgccc atagcgcggt gttggtgatg ccgaagcgga
     2041 cttccttgcc ctccatgttg ccgcccggct gcgatgccgt cgcatgctgg ttcaccgccg
     2101 ctgggaatag cggattgcct gccgcctccg cccagtacgt caccgtcacc ccggcgacaa
     2161 acaggatcgc catcgccgcc cacaccgccc agccatgcgc ctgcgagccc gtcatgcggc
     2221 ccagcgtgta ggtcaatccc gaggagatag cgaagatcgc aaccagttcg atcaagtttg
     2281 ttaccggagt gggattctcg aatggatgcg cgctgttcgc gttgaagaag ccgccgccat
     2341 tagtgccaaa ctccttgatg aactcctgcg acgcgaccgg cccttgcgcg atagtctggc
     2401 tggtgaccgt gtccatcacc ggcttgccgc tggcatccac cgcgggcttg ccgttcgcgt
     2461 tgacgtgctg cacctgttgc ggctcaagca gctttgccgt gtcgtagggg cgcaagttct
     2521 gcaccactcc ctgcgacacc agcaccagcg cgcccacgat acagaaaggc agcagcaccc
     2581 acaggcacgc gcgcaacatg tcgacccaga agttgccgat ggtctgcatc tgacggcgcg
     2641 cgattccacg gatgaacgcg atggccagcg cgatacccac tgctgcagaa acgaagttgt
     2701 gataggcgag cccggccatc tgcgtgaagt agctcatcgt ggtctcgccg ctatagttct
     2761 gccagttcgt gttggtggta aatgacgccg ccgtgttgaa cgcgagcgcc tgcgcaaccg
     2821 ccgtcagttt ttgcggattg aacggcagca tcgtttgcag ccgctccatc gcatacaaca
     2881 acagcatcga caccaggcta aagagcagca tcgcgacggc atattccgtc cagcgcatct
     2941 cgtgctcttc atcaaccccg gtcaggcgat agataacgcg ctcgatgggc cgcagcaccg
     3001 ggtccatgaa cgtgcgctcg cgctcgaaga cgcgcgccat gaagacgccg agcggcttcg
     3061 tgagcgcgag cacgaacgcg aagaacagca gaatctggag ccatccgttg gcggtcatgt
     3121 cagaacctct cgggccgcag gagcgcgtac atgaggtacg cgagcaggag cacggaaacg
     3181 acgattcccg cgacgaattc gaacgtcatc
//
LOCUS       DATIMH010000112         4302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5515481, whole genome shotgun sequence.
ACCESSION   DATIMH010000112 DATIMH010000000
VERSION     DATIMH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5515481"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..489)
                     /locus_tag="VLI90_01885"
     CDS             complement(109..489)
                     /locus_tag="VLI90_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12982.1"
                     /translation="MKHIVLALIAIALYPAVASAGHHGGGFGFFFSIGDGGRTYCAPP
                     APTCDPPCYSRCAFVSPGWQRTYAYAPQYESAAPVYCEPRIACVAPPPMIVYRSPIVV
                     YSPRVYCPAPYTAYYPRVTDCYPR"
     gene            622..1320
                     /gene="plsY"
                     /locus_tag="VLI90_01890"
     CDS             622..1320
                     /gene="plsY"
                     /locus_tag="VLI90_01890"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HSV12983.1"
                     /translation="MAGSSQQMLAGLAVAAYVAGSIPFGLIVGRMKGIDVRKAGSGNI
                     GASNVGRLLGKRFFFLVFFLDMLKGLIPMMIAAAIANRSGANSSLYAMVLLVGFATIA
                     GHMFSIFLRFKGGKGVATSAGVILGLWPYYTLPGLVAIGTFIVVVYLTRYISVASMAA
                     ACVFPVAYVSMGTMRGWDPFGRRLPLLVFAILMAVMIVYKHRGNIARLRAGTENRVGS
                     SKRANQTGEPPLAA"
     gene            complement(1376..1915)
                     /gene="arfB"
                     /locus_tag="VLI90_01895"
     CDS             complement(1376..1915)
                     /gene="arfB"
                     /locus_tag="VLI90_01895"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HSV12984.1"
                     /translation="MAGRTYHDAHGHDGARPCAVTNAMSAADDLIPNQPSSTTPGGIE
                     LAPGVRIADSNLRLQYARGGGPGGQNVNKLNTKAELWVPITAMIGLTERARERLVSLA
                     GHRLTQAGEIHIASDTRRTQEANRAEVFERLRELMIQAKHEPKRRRKTKPSRAAKQRR
                     LDAKKRRGDVKSKRRYTDD"
     gene            complement(1855..2466)
                     /locus_tag="VLI90_01900"
     CDS             complement(1855..2466)
                     /locus_tag="VLI90_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12985.1"
                     /translation="MTEAMYNHLSNVPDWVQSVVLPLNRWLHVVATATLVGGALFYEF
                     VIPKAIEDLKDETQLSVLGRVRWIFRRLVIFSTVVLCLTGAFETWLIWPSYTHRFAAA
                     RPWWGGHVIVGVTAMAMAVRITLGDRVPRHPIAWLRVSFVIMLVAIFLAALTRHIRLS
                     LQADAEAYGDQTILPDTSLPTMGPWPVEPTTMPTVTTAPAPAP"
     gene            complement(2463..2975)
                     /locus_tag="VLI90_01905"
     CDS             complement(2463..2975)
                     /locus_tag="VLI90_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12986.1"
                     /translation="MDLHAILIIFFRWLHVATACVAVGGVFFIRVIFPIGLRSLDAEP
                     ARTMLLRTRRAFKMVVHSAILLLLISGTYNAIQNWHGYTAAGPGLGHGLFGLHLLLAL
                     TVFGIALWLLTGAEPPADHQKWMGLNVVLLFLAIAAASTLKYAREHAKPLTNHQETAT
                     INVTSVTTQP"
     gene            complement(3148..3825)
                     /locus_tag="VLI90_01910"
     CDS             complement(3148..3825)
                     /locus_tag="VLI90_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSV12987.1"
                     /translation="MASILIIAPHPDDAELGMGGTILKLAKQRHQLHIVDMTNGEPTP
                     HGSPEIRARESAAASKVLGVERSNLGLKNREVQHTIEARHKLAGVIRQHKANWLFVPY
                     PIDAHPDHLAVTRIAEDARFDAKLTKCDTIPGVPCYPKRIIYYFCTHLLMNFTPNFCI
                     DVSDTIDRKVEAVRCYASQFQGASSHVPEMVRTINGYFGTRIGAPYAEPFYTHEMLGF
                     SGLDQLV"
BASE COUNT          790 a         1223 c         1456 g          833 t
ORIGIN      
        1 tctcccggag taccgggcga gggggacgga ggcgcgcgaa aaagcggcac gccgtgggta
       61 ctcggtcgtg ccgcccccct tcatctgaga ctccaaggtt gccgtgcgtt atcgcggata
      121 acagtcagtg acgcgcgggt agtacgcagt gtacggcgcg gggcaataga cgcgcgggga
      181 atagacgacg atcggggacc gatacacgat catcggcggc ggcgcgacac acgcgatgcg
      241 cggctcgcaa tacaccggcg ctgctgattc atattgtggc gcataggcat aggtgcgctg
      301 ccaaccgggt gagacgaacg cacacctgct gtagcatggc ggatcgcagg tgggcgcggg
      361 cggcgcgcaa taggttcgcc cgccgtcacc gatgctgaag aaaaagccga atccgccgcc
      421 gtgatgaccg gctgatgcga ctgcgggata aagtgcgatg gcaatgagcg ccaggacgat
      481 gtgtttcatg acgcggctcc ttcaccggct gcgtttgtcg ccggacgcca gttcagacaa
      541 acgtccccca ttcgcggtta gggaaaatca gaaaaatcga ctcggcgatt ttctgggcga
      601 tctcgggtaa aagcggtggc catggccggt tcatcccagc aaatgcttgc cggacttgcc
      661 gttgcggcct acgtcgcggg gtcgattccg tttggactca tcgtcgggcg gatgaaggga
      721 atcgacgtcc gcaaggccgg cagcgggaac atcggggcca gcaacgtcgg ccggctgctg
      781 ggtaagcggt tcttcttcct ggtgttcttt ctggacatgc tcaaggggct gatcccgatg
      841 atgatcgccg cggcgatcgc gaatcgatcg ggcgccaatt catcgctgta cgcgatggtg
      901 ttgctggtcg gcttcgcgac gatcgcgggg cacatgttca gtatcttctt gcggttcaaa
      961 ggcggcaaag gcgtcgcgac cagcgccggc gtgatcttgg gcctgtggcc gtactacacg
     1021 ctgccgggac tggtcgcgat cgggacgttc atcgtcgtcg tttacctgac gcgctacatc
     1081 agcgtcgcgt cgatggcggc tgcgtgcgtg tttccggtcg cgtatgtgtc gatgggcacg
     1141 atgcgcgggt gggacccatt cgggcggcgg ttgccgttgc tggtcttcgc gattctgatg
     1201 gcggtgatga tcgtctacaa acaccgcggg aacatcgcgc gattgcgggc gggaacggag
     1261 aatcgggtgg gttcgtcgaa gcgcgccaat caaacgggag aaccgccgct tgcggcttag
     1321 catttgtctg gttttcgtcg cgattggaaa cggatgctaa gccgcaagcg gcaattcagt
     1381 catccgtata gcgtcgcttg gatttcacat cgccccggcg ctttttcgcg tccaaccttc
     1441 gttgcttggc cgctcgactg ggcttggtct ttcgccggcg cttgggttca tgtttcgcct
     1501 gaatcatcag ctcgcgcaat cgctcgaaca cttcggcacg attcgcctcc tgcgtccggc
     1561 gggtgtcgga cgcgatgtgg atctcgcccg cttgggtcag tcgatgaccg gccaatgata
     1621 cgaggcgctc gcgcgcccgt tccgtcagtc cgatcatcgc cgtgatcggc acccacagtt
     1681 cggccttggt gttcagcttg ttgacgttct gcccaccggg tccgccgccg cgggcgtatt
     1741 gcaggcggag gttggagtcg gcgatgcgca cgccaggcgc cagttcgatg ccgccggggg
     1801 tcgtgctgct gggttgattc ggaatgagat cgtcggcggc ggacatggcg tttgtcacgg
     1861 cgcaggggcg ggcgccgtcg tgaccgtggg catcgtggta ggttcgaccg gccacggacc
     1921 catcgtcggc agggatgtat cggggaggat cgtctgatcg ccgtaggctt cggcatcggc
     1981 ctggaggctc aggcggatgt ggcgcgtgag ggccgccagg aagatcgcga cgagcatgat
     2041 gacgaagctg acgcgcagcc atgcgatcgg atgccgcggc acgcggtcgc ccagcgtgat
     2101 gcggaccgcc attgccatgg cggtgacacc gacgataacg tgcccgcccc accaaggtcg
     2161 cgccgcggcg aagcggtggg tatagctggg ccagatcaac caggtctcga aggcgccggt
     2221 caggcacaac acgacggtgc tgaagatgac cagccggcgg aagatccagc gcacgcggcc
     2281 cagtaccgat aactgcgttt cgtccttcag gtcttcgatg gccttgggga tgacgaattc
     2341 gtagaacagc gccccaccga ccagcgtggc ggtggccacg acgtgcaacc accgattgag
     2401 cggcagcacg accgactgca cccagtcggg cacgttggac agatgattgt acatcgcttc
     2461 ggtcatggct gggtcgtcac gctggtcacg ttgatcgtcg ccgtctcctg atgatttgtc
     2521 agtggtttcg catgttcgcg cgcatatttc aacgttgacg ccgcggcgat ggcgaggaac
     2581 agcagcacga cgttgaggcc catccacttc tgatggtccg ccggcggctc ggcgccggtg
     2641 agcagccaca gggcaattcc gaaaacggtc aaggcgagca gcaggtgaag gccgaacaat
     2701 ccgtgcccga ggcccggacc ggcggcggtg tagccgtgcc agttctgaat cgcgttgtag
     2761 gtgccgctga ttaatagcag caggatggcg gagtggacga ccatcttaaa cgcccggcgg
     2821 gtgcggagca gcatcgttcg cgcgggctcg gcgtcgagcg atcgcaggcc gatcggaaag
     2881 atcacgcgga tgaagaatac gccgcccacg gcgacgcagg cggtggcgac gtgcagccag
     2941 cggaagaaga tgatgaggat cgcgtgaagg tccatccgag cgcgatctta aatgctggag
     3001 ccccgcgtgt acatcgcatg tgcatgcgga gtgcgatgac cgcgtcgttt ataatagccg
     3061 ccgcggcctc gcggcgcgga cttcgagttg ccgcgcgtat tttctcaatc cgcgccgcga
     3121 ggccgcggcg gctaaacgac gtgcctttta gacaagttga tcgagcccgc tgaaaccgag
     3181 catctcgtgc gtgtagaacg gctctgcata gggcgcgccg atgcgcgtgc cgaagtagcc
     3241 attgatcgtc cgcaccatct ccggcacgtg gctgctggcg ccttgaaatt ggctggcgta
     3301 gcagcgcacc gcttcgacct tgcggtcgat cgtgtcgctc acgtcgatgc aaaaattcgg
     3361 cgtgaagttc atcagcaagt gcgtgcagaa ataatagatg atgcgcttgg gataacacgg
     3421 cacgccgggg attgtgtcgc acttggtgag cttggcgtcg aaccgggcgt cttcggcgat
     3481 gcgcgtgacg gccagatgat ccgggtgcgc atcgatcgga tacggcacga acagccagtt
     3541 cgccttgtgc tggcggatca cgccggccag cttatggcgg gcttcgatcg tgtgctgcac
     3601 ttcgcggttc ttcaggccca ggttgctgcg ctccacgccg agcactttgg aagccgcggc
     3661 ggattcgcgg gcgcggattt ccggcgaccc gtgcggcgtc ggttcaccgt tggtcatgtc
     3721 cacgatgtgc agctggtgcc gctgtttcgc gagcttgagg atggtgccgc ccatacccag
     3781 ctccgcgtcg tcgggatgcg gggcgatgat gaggatcgat gccatggtgc gatcattaaa
     3841 ggcgggtgac gcgcgaaagg ccactgggcg ttcatgtgat accaggctag ccgtacggga
     3901 gctggtccgg tcgtgatata tttgagagaa tgagctaata ttcgacgaac gcgcgcactg
     3961 gagggactgg aatgccatca gcaactaaaa agacttggat ttgcaaagcc acgcactcat
     4021 gagacgactt ttgccttcgc gtcccccggg acttggcgga ggagttacaa ctaattaacg
     4081 ggcaaaccgt gaagatccag gttgtgaagg gcgtagtcac cattcgtaag gcacgacctc
     4141 gcaggcgctg gagcgaggca aaactgctca aggggataac gccagatcga tgtggtccag
     4201 atctgatgcc tgacagagtc cgccgagagt tgatctgacg gcgcaggcat aggtttcatc
     4261 gatccagaca ccatttgagc ccgcccaacg agaaccgacc cg
//
LOCUS       DATIMH010000113         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3205230, whole genome shotgun sequence.
ACCESSION   DATIMH010000113 DATIMH010000000
VERSION     DATIMH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3205230"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VLI90_01915"
     CDS             <1..982
                     /locus_tag="VLI90_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV12988.1"
                     /translation="VTGNLNLGPAVRTFNIARGSAAADMMIAATISGGPGSGTALTKT
                     GAGILVLSGANTYTGNTLVNQGTLSVAAGGSLASASVAVASGATLKVDGTLSPTTAVS
                     ATGNVVFPGNTTGAAVTRTAGALTIGAGVTVQLGASSYSKTPAILSLASLAFGDGTAK
                     LDVSNDELITGGSTAANLRTVIVGSQLFSSAAGPGFGIGSIDVSGGKAEARFTLLGDA
                     NLDGAVDVGDLGVLATSYGITSSNIGWSQGDSNYSGGVDVGDLGSLATNYGSSLTTAP
                     AVVSDASEAMVAPIAQALAEVSSPTAVPEPGCLGILATIGGVAASRKRRR"
     gene            1482..2456
                     /locus_tag="VLI90_01920"
     CDS             1482..2456
                     /locus_tag="VLI90_01920"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HSV12989.1"
                     /translation="MRGFTFAVLLLLAIATLANSSGAEPPPPPERNLTVLIVDGMNNH
                     DWPRNTRILKTILERSGRFSVDVCTSPTNAASQDEWDKWRPAFARYDVILNNFNGGYK
                     ATDRQWPAAVEKDLENYITNGGGLVSYHAANNAFRNWPAYNEMIGLGWRDVNFGPSLI
                     VDKDQRLVVIPAGQGNKPGHPAEHDFQITLLNTDHPITRGMPKVWLHPHEQLTHGQHG
                     PAKNMTVLTYAWADDTKENELVDWVVPFGKGRVYTTMLGHLWKNGPDTALRCIGFQTL
                     FIRGVEWAATGQVTYPIPKDFPTADKIRMTPIFGSSANDVARTLNIAE"
BASE COUNT          536 a          878 c          824 g          437 t
ORIGIN      
        1 cgtcacgggc aatctgaacc tgggaccagc cgtgcgcacg ttcaacatcg cccgcggatc
       61 ggcggcggcg gacatgatga tcgccgcgac gatcagcggc gggccgggca gcgggacggc
      121 gttgaccaag acgggcgcgg gaatccttgt acttagcggc gccaacacct acaccggcaa
      181 tacactggtc aatcagggaa cgctatctgt agccgcaggc ggatcgctcg ccagcgccag
      241 cgtcgccgtc gccagcggcg cgacgttgaa ggtggacggc acgctctcgc ccaccaccgc
      301 cgtcagcgcc accggcaacg tcgtcttccc cggcaacacc accggcgccg cggtcacgcg
      361 cacggccggc gcgctgacga tcggcgccgg tgtcaccgtg cagctagggg cctccagtta
      421 ctcgaaaacg cccgccatcc tcagcctcgc atcactggcc ttcggcgatg gcaccgccaa
      481 gctcgatgtg tccaacgatg agctgatcac cggcggcagt accgccgcga acctgcgcac
      541 ggtcatcgtc gggtcgcagc tgttcagcag cgcggccggg ccgggatttg gcatcggctc
      601 gatcgatgtc tccggtggca aggcggaggc gcgcttcacg ctgctgggtg acgcgaacct
      661 cgatggggcc gtcgatgtcg gcgatctggg cgtgctggct acgagctacg gcattaccag
      721 cagcaacatc ggctggtccc aaggcgacag caactacagc ggcggcgtgg acgtcggcga
      781 tctgggcagc ctcgccacca actacggctc cagcctgacc accgccccgg cggtcgttag
      841 cgacgcgagt gaggccatgg tcgcgccgat cgcgcaggcg ttggcggagg tgagttcgcc
      901 cacggcggtg ccggagccgg gttgcctggg aatcctcgcg acgatcggcg gggtggctgc
      961 gagccggaaa cgtcggcggt gagcaagtat cgctgtgaag tggttcggtg cgagggcgct
     1021 gtcgtcctga gcgcaggcgt acgcgccgga gtccaaggat ctaaagtaag tgcgacaaat
     1081 taaatccgat tggtcatgga gacgctgaaa acgatcgccc cctccacgcc gggttcgaag
     1141 gtactccgac gaaccggatt cttctgcagc acgcgaaaag aatctcgctt cgtcgcgagt
     1201 acgctccgga cccggcgtgg gagcgtttct tcaacgacgg tcgttgcgaa attgatcgtt
     1261 ccgggcgcaa tcggaaaaac ttagcagcgc ttccttagac ttagaactag tcgcccaaaa
     1321 agagtccacg accgtcgagc acccttttag cggcggcccg aagggccgag cgggcgttca
     1381 gacggcgctg aacgcttgac cgatcgcgat ctgcacctcc aaggatcgca actcgcgccc
     1441 gcgaatcttg ataggatgca tctcccttca cggaggcgct cttgcgcgga ttcacgtttg
     1501 ccgtcctcct cctcctcgcc attgcaacgc ttgcgaattc gtccggcgcg gaaccaccgc
     1561 cgccgccgga acgcaacctc actgtcctca tcgtcgatgg catgaacaac cacgactggc
     1621 cgcgcaatac gcgcattctc aaaaccatcc tcgagcgcag cggccgcttc agcgtcgatg
     1681 tctgcaccag cccgacgaac gccgcttcgc aagatgaatg ggacaaatgg cggccggcgt
     1741 tcgcgaggta cgatgtcatc ctcaataact tcaacggcgg ctacaaagcc accgatcgcc
     1801 aatggcccgc ggcggttgag aaggatctcg aaaactacat caccaacggc gggggactcg
     1861 tcagctatca cgccgccaac aacgcgtttc gcaattggcc ggcgtacaac gagatgatcg
     1921 gcctcggttg gcgcgacgtg aacttcggcc cctcgctcat cgtcgataaa gaccagcgcc
     1981 tcgtcgtcat ccccgccggt caaggcaaca agcccggcca tccggcggaa cacgattttc
     2041 aaatcacgct gctgaacacc gatcacccga tcacccgcgg catgcccaag gtctggctgc
     2101 acccgcacga gcagctgacc cacggccagc acggcccggc gaagaacatg accgtcctca
     2161 cctacgcctg ggctgatgac acgaaggaaa acgagcttgt ggactgggtg gtccccttcg
     2221 gcaaaggccg ggtctacacg acgatgctcg gccacctgtg gaagaacggg ccggacacgg
     2281 ccctgcgatg catcggcttc caaaccctgt tcatccgcgg cgtcgaatgg gccgcgacgg
     2341 ggcaggtgac ttatccgatt ccgaaagact tcccgacggc ggataaaatc cgcatgacgc
     2401 ccatttttgg aagctccgcc aacgacgtcg cccgcacgct aaacatcgca gaatagttcc
     2461 tggtgatccg tgcaacctgc gcggcgtgtt tgactgccac tcctatcatc gcggtagatc
     2521 ggagatcaca tgatcaacat gcccagcgac atcgcgttca cccccgccgt caaagcggtg
     2581 cagacaggca agaggcggag gtggaaaggt tacgcgcggg tgggtgatcg agcgccagcc
     2641 ggacgaggta tctaaccacg ccgggttcgg aggta
//
LOCUS       DATIMH010000114         3893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2338009, whole genome shotgun sequence.
ACCESSION   DATIMH010000114 DATIMH010000000
VERSION     DATIMH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338009"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VLI90_01925"
     CDS             complement(<1..746)
                     /locus_tag="VLI90_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV12990.1"
                     /translation="MKPVTSLGSTIVLRLRDLQYLLSVLVAIALLAVQPRYWPRTVRN
                     VLARQILFTGVEALGLIFAVASVAGISVVLQAQVWLGKVGRRDLLGPLLVVVVVREFA
                     PVLASLVVIIRSASAVASEMANMQLAGEVRLLDAQGLDPLVYLVMPRVVGLSVCAFGL
                     SILFVVVSLVSGYLFVTLLGHAIRPRDFADSVFGALGPVDMLNLLGKSVIPAALTAVI
                     CCTEGLSVGAGGTEIPRATSRGLARSVVAM"
     gene            1350..2030
                     /locus_tag="VLI90_01930"
     CDS             1350..2030
                     /locus_tag="VLI90_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12991.1"
                     /translation="MKTSPATASLTPMNGPYPGEDGLNQILAHQYGGVFSAAGSNFTN
                     GAITAQRIDDASDQTWNRHFVSAHVVGAFSAYAQSFGVFAGSGGGAFQKFFDVYGWGY
                     NVTGSASNLNLGGTYRLARGGGGDLVSSKPGNNVDLKDRMVTYKVNGLGDHTNTYMVF
                     FEDFHDFDYNDLSVQLKTAAVFGPPAGVKAVPLPPAVWMDLSTLAGTGVVGGARRLRR
                     RVRVSAGR"
     gene            complement(2031..2237)
                     /locus_tag="VLI90_01935"
     CDS             complement(2031..2237)
                     /locus_tag="VLI90_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12992.1"
                     /translation="MNKRLILVISILAVPVIAVLPRVWRWSPAVAWLFGLAICVWSVW
                     LMVLYLKWARQLGNTSPGEPPMHI"
     gene            complement(2371..2664)
                     /locus_tag="VLI90_01940"
     CDS             complement(2371..2664)
                     /locus_tag="VLI90_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12993.1"
                     /translation="MVYSTKEGYRFRVRCANADSVNLLGDFNDWSTTSHPMTPTEDGA
                     WELALDPGAGPHRFAYFVLLAKRDGDGERIDALASVVLQEDVHGSSPMRRMAS"
     gene            2836..3120
                     /locus_tag="VLI90_01945"
     CDS             2836..3120
                     /locus_tag="VLI90_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HSV12994.1"
                     /translation="MTPSNDQQSEVIVAGADWCGDTRATLAYLQELGVPFNYVNVDED
                     ESAADWVREQNSGKLKLPTVDVGGLILSIPDAEQLQAALDDRNLLGDGRT"
     gene            3183..3872
                     /locus_tag="VLI90_01950"
     CDS             3183..3872
                     /locus_tag="VLI90_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HSV12995.1"
                     /translation="MNPPDDDLAHTSALAITIGRQPGSGGSDLGRAIATRLGIAYLDQ
                     EILREAARQLGVQDQSLTDRGERVTKFWERLLRAFALGAPEETYTAYPERSEVVASIP
                     DVDLFNAQSRAMRELVQLGDSVVIGHAGFYVLRDHPCHVKVFVHAKAELRAERISRER
                     NVSIADARIMVRQADDERRRFIEHISGCEWTNVRNYDLCIDSGRVSNALAEEMIVRLV
                     NERRQQCAGAR"
BASE COUNT          795 a         1285 c         1158 g          655 t
ORIGIN      
        1 aacatcgcga caacggatcg cgccagtccc cgactggtcg cgcgcgggat ttccgtgcca
       61 ccggcgccaa cgctcaaccc ttccgtacag cagatgaccg ccgtcaacgc cgcggggatg
      121 acgctcttgc ccagcaggtt cagcatgtcc accgggccca gcgcgccgaa aacgctgtca
      181 gcgaaatccc gcgggcgaat tgcgtggccc agcagcgtca cgaacaggta accgctcacc
      241 agcgagacga cgacgaacag gatgctcaac ccaaacgcgc acaccgacag gccgacgacg
      301 cgcggcatca cgaggtagac cagcgggtcc agcccctgtg catcgagcaa tcgcacttcg
      361 ccggccagct gcatgttggc catctccgac gccaccgcgc tggcggatcg aatgattacc
      421 accaggctcg ccagcaccgg cgcgaattcg cgcacgacga cgaccaccag cagcggtcca
      481 agcaaatcac gccggccaac cttgccgagc cagacctgcg cctgcagcac gaccgaaatg
      541 cccgcgaccg atgccacggc gaagatcagg ccgagcgctt caacgccggt gaacaggatc
      601 tgtcgcgcca gcacgttgcg gacggtgcgt ggccaatagc gcggctgcac cgcgagcagc
      661 gcgatcgcca ccagcaccga aagcaggtac tgcagatcgc gcagccgcag cacaatcgtg
      721 ctgccaagac tggtcactgg cttcatcacg ggaccgaccc cgctgctgaa ttctcgaacg
      781 cactgatacg aaatggacgc cctcgcacta tcgcggcgac tgattctgcc gagatcgatc
      841 gcgcatgtcc aattctttct ggagtcctga tcggaagcgg attatcggac cgcacatttg
      901 cgtaggcgac cgtaccgacg tgggtaacaa gcggaacgcc gcggcaacga tggcggtgat
      961 cctgcgtgtc cgatgaccaa ttggccgctc agatcacccc ggccgttccc ttcaattata
     1021 aacaagcccc cgttttcaca tcacccgtcc gaataacgcg tcgaaggcgt gacacaaacg
     1081 tgttacacgc ggcacagagc gtccatcttc agtcggatat atgcggacgc gcgggtgatt
     1141 tccccatcgc tccgcgcgcc ggtgctatag ttcaaacagt cccacaaagc tgtggctgat
     1201 cgagcagctg ccccttcgag ggtggccgcc ggttcaattc ataaagccgt tcgacaaatt
     1261 cccctcacgc gaaccaacgg aggtccaaga tgtgcagcaa aactgcccgc gctcttgttc
     1321 tatccttatc cgccgcctcg gcgatgcttt tgaaaacatc acccgcgacg gcgagcctga
     1381 cgccgatgaa cgggccctat ccgggtgaag atggcctgaa ccagatcctc gcccaccaat
     1441 acggcggcgt gttcagcgcc gccggatcga acttcaccaa cggcgcgatc accgcgcagc
     1501 gcatcgacga cgcgagcgat cagacgtgga acaggcactt cgtctccgcg cacgtcgtcg
     1561 gcgcgttctc cgcctacgcg caatccttcg gcgtcttcgc cggcagcggc ggtggcgcgt
     1621 ttcaaaaatt cttcgacgtg tacggatggg gctacaacgt gactggatcg gccagcaatc
     1681 tgaacctcgg cggcacctat cgcctggccc gcggaggcgg cggtgatctg gtcagcagca
     1741 agcccggtaa caacgtcgat ctgaaggatc ggatggtgac gtacaaggtg aacggcctcg
     1801 gtgatcacac gaacacgtat atggtcttct tcgaggactt ccacgacttc gactacaacg
     1861 acttgtcggt gcagttaaag acggccgcgg tgttcgggcc gcctgccggt gtgaaagcgg
     1921 tgccgctgcc gccggcggtg tggatggacc tcagcacgct tgcaggcacg ggcgtcgtgg
     1981 gtggcgcccg gcgcctgcgg cggcgcgtgc gcgtcagcgc cggtcgatag tcagatgtgc
     2041 atcggtggct cgccaggcga ggtgtttccc aactggcgcg cccacttgag atacagcacc
     2101 atcagccaga cggaccagac gcagatcgcg aggccgaaga gccaggccac cgccggactc
     2161 caccgccaaa cccgcggcag caccgcgatg acgggcacgg ccaatatgct gatgacgaga
     2221 atgagacgct tgttcacgac agaagcatag ctctggtcca cgcagccgtc acatttgtga
     2281 aatgcagaat gcagaatgca aattggaagt gggaattttg cacccttctt ccactgtgca
     2341 ttctgccttc atcattccgg attcgagtat tcagctcgcc atccgccgca tcggcgacga
     2401 cccgtgaacg tcctcttgca gcacgaccga cgccagcgcg tcaatgcgct caccatcgcc
     2461 gtcgcgcttg gccagcagca cgaagtacgc gaagcggtgc ggccccgccc ccgggtccag
     2521 cgccagctcc cacgccccgt cttccgtcgg cgtcatcgga tgcgaggtcg tggaccagtc
     2581 gttgaaatcg ccgagcaaat tcacggaatc cgcgttcgcg catcgcaccc gaaagcgata
     2641 accttctttt gttgaataca ccatggaaca cacacccggt tttggctgca ggcgacttcc
     2701 attcgcccgc tgctaacatt cataggcgac tcgccgcgcc gaccgccatt acgagaaacc
     2761 ccaaacaccg cggcgaaatt ccttcgtata accttgcggt tgccgcggca taaatttggc
     2821 ttacgagcac ttcatatgac cccatcgaac gaccagcaat ctgaagtgat cgtcgccggc
     2881 gctgactggt gcggcgacac gcgcgccacg cttgcatacc tgcaggagct gggcgtgccc
     2941 ttcaattacg tcaacgtcga cgaggacgaa agcgccgccg attgggtccg ggagcagaac
     3001 agcgggaagc tcaagctgcc cacggttgac gtgggcgggc ttatcctgag catccccgat
     3061 gccgagcagc tgcaggcggc gctggatgac cgcaatctgc tcggtgatgg aagaacatga
     3121 ggtggccccg ccgcggtttg cgataatatg cggggcgttt ggcggaccgg agcaacctgc
     3181 agatgaaccc gcccgacgac gatttggcgc acacgtccgc cctggcgatc acgatcggcc
     3241 ggcaacccgg cagtggtggg tccgatcttg gccgcgccat cgccacgcgg ctgggcattg
     3301 cgtatctcga ccaggaaatc ctgcgcgaag ccgcgcgcca gctcggcgtg caggaccaaa
     3361 gtctcaccga tcgcggcgag cgcgtcacca aattctggga acgcctgctg cgggcgttcg
     3421 cattgggcgc gccagaggaa acctacaccg cctatcccga acgttccgaa gtcgtcgcgt
     3481 cgattcccga tgtggacctg tttaacgccc aatcgcgcgc gatgcgcgag ctggtgcagc
     3541 tgggcgattc cgtcgtcatc ggccacgccg ggttttacgt gctgcgcgat catccctgcc
     3601 atgtgaaggt cttcgtacac gccaaggccg agctgcgcgc cgagcgcatc agccgcgagc
     3661 gcaacgtttc cattgccgat gcgcgcatca tggtccgcca ggcggatgac gagcgccggc
     3721 ggttcatcga gcacatctcc ggctgcgagt ggacgaacgt ccgcaactat gacctgtgca
     3781 tcgacagcgg ccgagtgagc aatgcgctgg cggaagagat gatcgtgcgg ctggtgaacg
     3841 aacgccgcca gcaatgcgcc ggcgcacgat gagccgatca gtgcgctgcc gcg
//
LOCUS       DATIMH010000115         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3782552, whole genome shotgun sequence.
ACCESSION   DATIMH010000115 DATIMH010000000
VERSION     DATIMH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3782552"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..521)
                     /gene="purE"
                     /locus_tag="VLI90_01955"
     CDS             complement(12..521)
                     /gene="purE"
                     /locus_tag="VLI90_01955"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSV12996.1"
                     /translation="MTTNAANPAKPPLVGVIMGSRSDWETMRHADEVLKQFDVPHECK
                     VVSAHRTPQWMVQYASEAERRGLQLVIAGAGGAAHLPGMVAANTILPVIGVPVQSAAM
                     NGLDSLLSIVQMPGGIPVATTAIGRAGAMNAGLLAVAILANHDAALRQKLMDYRDAQT
                     QKVMGESLE"
     gene            complement(645..1073)
                     /locus_tag="VLI90_01960"
     CDS             complement(645..1073)
                     /locus_tag="VLI90_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSV12997.1"
                     /translation="MSRPRQPDGRVHGVVVACRRGDGRWLCIRRSATVAAPLKVCFPG
                     GAMEVGEDQATAAVREMREELGITINPLRCVWRWDSPTTELTLWGWIADLPDEPLRPD
                     PTEVSEVLWLTDAAVGAHPEGLPSNQSFIECLVRELGKTL"
     gene            complement(1126..2031)
                     /locus_tag="VLI90_01965"
     CDS             complement(1126..2031)
                     /locus_tag="VLI90_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV12998.1"
                     /translation="MFDRATERLQHKTWASFNTVERLLLVAGCTACAFLAAALSRAAG
                     VPPTPHFAGSLLAAPDAFTAVLVAAIAVAACTALGTVIAGFLRLEVGLICAAIGLAAL
                     SARGGSMRAVLQYASSPSIYAALVVETLILFGLLAAAYLLLRRLFPLIWSARLPAEAI
                     AKPADHDVLSEPSDIAFAIAVYAIAMIVCESLLLQSDAKKQAMASVAVSSIVATLAAC
                     QIAPLVEGVWYWLVGPCVVAVVGYALAYASPEGWQVGEVHGMLGALARATPLDYASLG
                     VAGAILGYWTARKWKEEGEFGSSTE"
BASE COUNT          364 a          687 c          688 g          332 t
ORIGIN      
        1 ctgccgcgcc gtcactccaa actttcgccc atcaccttct gcgtctgcgc gtctcgataa
       61 tccatgagct tctgccgcag cgccgcatcg tgattcgcaa gaatcgccac cgccagcagc
      121 cccgcgttca tcgcgccggc gcgaccgatc gccgtcgtcg caacggggat cccgccgggc
      181 atctgcacga tcgacagcag tgaatcgagg ccgttcatcg ctgcgctctg caccggcacg
      241 ccgatcaccg gcaggatcgt gttcgccgca accatgcccg gcagatgagc cgcgccaccg
      301 gcgccggcga tgaccaactg caatccgcgg cgttccgcct cactcgcata ctgcaccatc
      361 cactgcggcg tgcggtgcgc cgaaaccacc ttgcactcgt gcggcacgtc gaactgcttc
      421 agcacctcgt ccgcgtgccg catcgtctcc caatcggacc tgctgcccat gatcacgccc
      481 acgagcgggg gcttggccgg gtttgctgcg ttggtggtca tgctgcggcg cggatcgtaa
      541 tggaagcgcg ggcgggctgc aaaaagctgc accttgtcat gtagctgcga cgcctgcgtc
      601 acctcttctt gcgatgcgaa gggaaggcga cgcaggcgtc gcggctacaa agttttgccg
      661 agctcgcgca caaggcactc gatgaagctc tgattgctgg gcagcccctc cggatgcgcg
      721 ccaaccgccg cgtccgtcaa ccacagtacc tcgctcactt ctgtcgggtc tgggcgcaat
      781 ggttcatccg gcagatccgc gatccatccc cacagcgtca actcggtcgt cggcgaatcc
      841 catcgccaga cgcagcgcag cggattgatc gtaatgccca actcttcgcg catttcgcgc
      901 acggcggccg tggcctggtc ttcgccaacc tccatcgcgc cgccggggaa gcagactttc
      961 agcggggctg ccacggtggc gctgcggcgg atgcagagcc agcggccatc gccgcgccgg
     1021 caggcgacga cgacgccgtg aacgcgacca tcaggttggc gggggcggga catctttttg
     1081 aatgcagaag gatgaatgca gaatgaagaa tgggtaagag ccgagctact cggtggaact
     1141 gccgaactcg ccttcttctt tccacttcct cgcggtccag tatccgagga tcgcgccggc
     1201 gacgccgagg ctggcgtagt ctagcggtgt ggcgcgggcg agcgcgccga gcatgccgtg
     1261 gacctcgccg acctgccagc cttcggggga ggcgtaggcg agggcgtaac cgacgaccgc
     1321 gacgacgcac gggccgacga gccaatacca cacgccttcg accagcgggg cgatctggca
     1381 cgcggccagc gtggcgacga tcgatgacac cgcgacggac gccattgcct gcttcttcgc
     1441 gtcgctctgc agcagcagcg attcgcagac gatcatcgcg atcgcgtaga cggcgatcgc
     1501 gaacgcaatg tcgcttggct cgctcaacac atcgtgatcg gccggtttgg cgatcgcctc
     1561 ggcgggcagg cgggcggacc agatcagcgg gaatagtcgc cgcagcagca ggtacgcggc
     1621 ggcgaggagg ccgaagagga tgagcgtttc gacgaccagc gccgcgtaga tcgacgggct
     1681 cgatgcgtat tgcagcacgg cgcgcatcga tccaccgcgg gcggataacg cggcgaggcc
     1741 gatggcggcg cagatcaggc cgacttcgag gcgaagaaat ccggcgatga cggtgcccag
     1801 cgccgtgcac gccgccaccg cgatggcggc gactagtaca gcggtgaagg catcgggcgc
     1861 ggcaagcaag ctgccggcga agtgcggcgt gggtggcacg ccggcggcgc ggctgagcgc
     1921 ggcggcgagg aacgcgcacg cggtgcagcc ggcgacgagc agcaggcgct cgacggtgtt
     1981 gaacgatgcc caggtcttgt gctgaaggcg ttcggtggcg cgatcaaaca tttcttattc
     2041 cctgcgaagc tgggatcaca cttggtccct g
//
LOCUS       DATIMH010000116         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2338744, whole genome shotgun sequence.
ACCESSION   DATIMH010000116 DATIMH010000000
VERSION     DATIMH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338744"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..2571)
                     /locus_tag="VLI90_01970"
     CDS             complement(133..2571)
                     /locus_tag="VLI90_01970"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HSV12999.1"
                     /translation="MMALLLPSPPMTMTLASTLVWGAAQWRAVAVPLVALLLVLLLWG
                     YSRAKLQPWVRVVAASVKLLAVVILALCLLEPLFSGTRAQPGANLFVVLADNSQSMTL
                     RDRDAPQTRAEQLKSLTAKPAATWLARIGQDFDLRQYAFDSQLHANVDLPALAFDGGS
                     SNLGAALQRLARQYHGRPLAGIMLLSDGAATDAETIDRIVAQAKESGTPLPPIYPVVL
                     GRDAPADDVNLQRVTVSQTNFEDTPVTVSAQITTTGYAGRMLAAQLVDESGKAIDEQK
                     VKADKDGEPVTVRFRLRPQQPGVSFYRVRVAADGQLPQLDAAKESSEATLANNTRLVA
                     VDRGKGPYRVLYVSGRPNWEFKFLQRAVLEDDQVQLVDMVRIAKREPKFNYINAGDRA
                     NPLFRGFAGTDSEQVEQYDQPVIVRLGTQNENELRGGFPQSPEDLYHYHAIIIGDLES
                     GFFTQDQMLLIKEFVRQRGGGLLMLGGQESFKEGNYDRTPIGDLLPVYLNDVVSLPED
                     ARYRLQLTREGWLEPWIRLRPEEEAERARLASMPQFMTMNAVRGIKPGATVLATATVE
                     GAAGADASAIVPALVEQRFGRGRSAAMMVGDLWRWTLQRQADAPDDLPKAWRQMVRWL
                     VSDVPQRVELAVTPRREADDPDGMLAIAVRVRDSRYDPQDNAAVTVRVTTPDGHNVDL
                     PAEASAQEAGLYEAAYMPRQPGAYRAQATAVAPDGSEIAKVQSGWTADPAADEFRDLR
                     PNRALLERIARDTGGQVVAAADLQRFAESLPMRHADVTEPDIRPAWHQSWVFLLAIAC
                     LCAEWGLRRWKGLP"
     gene            complement(2568..>3173)
                     /locus_tag="VLI90_01975"
     CDS             complement(2568..>3173)
                     /locus_tag="VLI90_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13000.1"
                     /translation="QRGQEGRGLRGGGGGLRGTAQQDANQQPSQQQPDDQQQQQPQEA
                     AQNSQRGGQRAGQQQANAGNRNIDGGDDQANPTRGFGGAGGNANHGPLTGEDYRDWSD
                     RLRDVEEMVSDPRLRAEAARIRERARDVRIEFKRHSLPPNWNTVDLQLGQPLAQLRDE
                     VDQELLRHESKDALVPIDRDAVPPEYAEQVRQYYERLGSGR"
BASE COUNT          501 a         1190 c          936 g          546 t
ORIGIN      
        1 cagagcccgt caccatcgct cgtggcaccg cagtcagcgt ccatatgaac gctaacgcca
       61 cggccgcggc agactggaat gtcggcccca cccacagagc ggaataccta ccccgccgta
      121 aacgccatcc catcacggca accccttcca tcggcgcaat ccccactcgg cgcacaggca
      181 cgcgatcgcc agcaggaaca cccacgattg atgccacgcc gggcgaatgt ccggctccgt
      241 cacgtccgcg tgccgcatcg gcagcgactc ggcaaagcgc tgcagatccg ccgccgcaac
      301 cacctgtccg ccggtgtcgc gggcaatccg ttccagcagc gcccgattcg gccgcagatc
      361 gcgaaactca tccgccgccg ggtcggccgt ccatccgctc tgcaccttcg cgatctcgct
      421 cccgtctggc gccaccgcgg tcgcctgcgc gcgatacgcc cccggttgcc gcggcatata
      481 cgccgcctcg tacaaacccg cctcttgcgc gcttgcctcg gccggcagat cgacgttgtg
      541 accgtccggc gtcgtcacgc gcacggtgac ggccgcgttg tcctgtggat catagcgcga
      601 atcgcgcacg cgcaccgcga ttgccaacat gccgtccgga tcatccgcct cacgccgtgg
      661 cgtcaccgcc agctcgacgc gctgcggcac atccgacacc agccagcgca ccatctgccg
      721 ccacgccttg ggcaggtcgt ccggcgcatc cgcctgccgc tgcagcgtcc agcgccacag
      781 gtcgccgacc atcatcgccg cgcttcgccc gcgaccgaag cgctgttcga ccagcgcggg
      841 cacgatcgcg ctcgcgtcag ccccggcagc gccttccacc gtcgccgtgg ccagcaccgt
      901 cgcgcccggc ttgatcccgc gcaccgcgtt catcgtcatg aactgcggca tgctcgccag
      961 ccgcgcgcgc tccgcctctt cctccggccg caggcgaatc cacggctcca gccaaccctc
     1021 gcgcgtcagc tgcagtcgat agcgcgcatc ctccggcaag ctcaccacgt cgttcagata
     1081 caccggcagc agatcgccga tcggcgtgcg gtcgtagttg ccttccttga acgattcctg
     1141 cccgcccagc atcagcagtc cgccgccgcg ctggcggacg aattccttga tcagcagcat
     1201 ctggtcctgc gtgaagaagc cggactccag gtccccgatg atgatcgcgt gatagtggta
     1261 aagatcttca ggcgattgcg gaaatccgcc gcgcagttcg ttctcgttct gcgtgccgag
     1321 gcggacgatg accggctgat cgtactgctc gacctgctca ctgtcggtgc cggcgaaccc
     1381 gcggaacagc ggattggcgc gatcgccggc gttgatgtag ttgaacttcg gttcgcgctt
     1441 ggcgatgcgg accatgtcga ccagctgcac ctgatcgtcc tccagcaccg ctcgctgcag
     1501 gaacttgaat tcccagttcg gccggcccga gacgtacagc acgcgatacg gccccttgcc
     1561 gcgatcgacc gccacaagcc gcgtgttgtt tgccagcgtc gcctcgctcg actcttttgc
     1621 ggcatccaat tgcggcagct gaccatccgc cgcgacgcgc acgcgataaa acgaaacacc
     1681 cggctgctgc gggcgcaacc gaaatcgcac ggtgaccggc tcgccatcct tgtccgcctt
     1741 caccttctgc tcgtcgatcg ctttgcccga ttcatccacc agctgcgccg cgagcatccg
     1801 cccggcgtag ccggtggtgg tgatctgcgc gctcaccgtc accggcgtgt cttcgaagtt
     1861 ggtctgactg acggtgacgc gctgcaggtt cacgtcatcc gccggcgcgt cgcggccaag
     1921 gacgaccgga tagatcggcg gcaggggagt tccagattcc ttcgcttgtg cgacgatgcg
     1981 atcgatcgtc tccgcatcgg tcgccgcgcc gtcactcagc agcatgatgc ccgcgagcgg
     2041 ccggccgtga tattgccgtg cgagtcgctg caacgcagcg ccgagattgg acgagccgcc
     2101 gtcgaatgcc agcgccggca gatcgacgtt cgcgtgcagc tgcgaatcga acgcgtattg
     2161 acgaagatca aaatcctgtc cgatgcgcgc gagccacgtc gccgccggtt tggccgtgag
     2221 cgatttcagt tgctcggccc gcgtctgcgg tgcatcgcgg tcgcgcagcg tcatgctctg
     2281 gctgttgtcg gcgaggacga cgaagagatt tgccccgggc tgtgcgcgcg tgccgctgaa
     2341 cagcggctcc agcaggcaca gcgccaggat gacgaccgcc agcagcttca ccgatgccgc
     2401 gacgacgcgc acccacggct gcaatttcgc gcgcgaatat ccccacagca gcaggaccag
     2461 cagcagcgcc accagcggaa ccgccaccgc gcgccattgc gctgcgcccc acacgagcgt
     2521 cgatgcgagc gtcatcgtca tcggcggcga cggcagcagc agcgccatca tcgaccgctc
     2581 cccagccgct cgtaatattg ccgtacctgc tcggcatatt ccggcggcac cgcgtcgcga
     2641 tcgatcggca ccagcgcatc cttcgactcg tgccgcaaca attcctggtc cacctcatcg
     2701 cgcagctgcg ccagcggctg gcccagctgc aggtccaccg tgttccaatt cggcggcagg
     2761 ctgtgacgct tgaattcaat gcgaacatcg cgagcgcgct cgcgaatccg cgccgcttcg
     2821 gcgcgcagcc gcgggtcgga caccatctcc tcgacatcgc gcaatcgatc agaccagtcg
     2881 cggtaatctt cgccggtcaa cggcccgtgg ttggcgttgc cgccggcgcc gccgaaccca
     2941 cgcgtgggat ttgcctgatc gtcaccgcca tcgatgttcc gattccccgc gtttgcctgc
     3001 tgctggcccg cgcgctgccc accgcgttga ctgttctgcg ccgcctcctg cggttgctgt
     3061 tgctgctgat catccggctg ctgctgcgat ggttgctgat tcgcatcctg ctgagccgtc
     3121 ccgcgcaacc cgccgccgcc accgcgcaat ccgcggcctt cctggccccg ctg
//
LOCUS       DATIMH010000117         1945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3494771, whole genome shotgun sequence.
ACCESSION   DATIMH010000117 DATIMH010000000
VERSION     DATIMH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3494771"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VLI90_01980"
     CDS             <1..303
                     /locus_tag="VLI90_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13001.1"
                     /translation="LPAGVLPDGNYHLAIAAASVADDNGHALRQNATLDFFTLDGDAN
                     HDRVVDVADLGRLATNYNVTLGMTFSDGDFNYDGKVDVADLGRLATNYGKTLAKPG"
     gene            624..>1945
                     /locus_tag="VLI90_01985"
     CDS             624..>1945
                     /locus_tag="VLI90_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV13002.1"
                     /translation="MTATVAALAAATSFGVSRLAWAGNTWTGNGASANWSDDNNWGGA
                     QPAYGTLSFNLGGTQGTTSIVDQSYSMNQLLWTGTSAWTVNSANAAVISLFDLSGTQA
                     KVENQSSGLVTLNAPITFAATAGANWGEINAVNGDLTFGTGMLTVNGSQVAGIRSFTG
                     AGRTVTFNNTVSASGKYLALTASTSGTITIGSSANVTIADLYIMNGGTLNLGGSTLTN
                     DSGTGVRLGGDFGTTGTQDLTKGGTFNLTATGGGTTYAGVINSVALNTSGALAVNSNN
                     TSGANTLSGHVALDSALQFTQASGGTLNITQARAGGVGTTTGTDIKGQSLTLTPASGG
                     TINFSGDIYNSTGAGSVLMNGAGTVTLGGANTYTGATTISAGILSVTGAINSANAANV
                     GRISVANTSGNAELLISGTVRATNTTAPSLLVGSVSGANGTIIVNIGGT"
BASE COUNT          387 a          649 c          612 g          297 t
ORIGIN      
        1 ctgcctgcgg gcgtgctgcc ggacggcaac tatcacctcg ccatcgccgc cgccagtgtg
       61 gcggacgaca acggccacgc gctgcggcaa aacgcgacac tggatttctt cacgctggat
      121 ggcgacgcga atcacgatcg cgtggtggac gtcgccgact tgggccggct ggcgacgaac
      181 tacaacgtca cgcttggtat gaccttcagc gatggcgact tcaattacga cggtaaagtg
      241 gacgtcgcgg acctcgggcg gctggcgacc aactacggca aaaccctcgc caagccgggg
      301 tgaggcgcgc tggttgcgac gaagggagcg actgctgtgc gccgccgcgt tagctcgcgt
      361 cgtccgcctg aacgaccttg attgcttccc aaaattcggt tgcgtcacca ctcccgcagg
      421 ccgcgttggc ccgatccgtg ccctccccgc aaaacgtggg tcattttgcg tctaattgcg
      481 tgaattgtgg ataacggttt ttcaataccc caactacggt actggccgtt gaatgggagt
      541 tgaggcgcgg cgcgcgcagc gtcgcgcccg cgcgcggggc agaggagagg caattcatgc
      601 gggccagacg gatgcgacgc ggcatgaccg caacggtcgc ggcattagcc gccgccacga
      661 gcttcggcgt ttcgcgcttg gcctgggccg gcaacacgtg gaccggcaat ggcgcgtcgg
      721 ccaactggag cgacgacaac aactggggcg gcgcccagcc cgcttacgga acgctgagct
      781 tcaacctcgg cggcacgcaa ggcaccacca gcatcgtcga tcagtcgtac agcatgaacc
      841 agctgttgtg gaccggcacg agcgcgtgga ccgtcaacag cgccaacgcc gcggtcattt
      901 cgctatttga tctcagcggc acccaggcga aggttgagaa ccagagcagc ggcctggtga
      961 cgctgaacgc gccgatcacc ttcgccgcca cggccggcgc gaattggggc gagatcaacg
     1021 ccgtcaacgg tgatctcacg ttcggcaccg gcatgctcac cgtcaatggc tcgcaagtcg
     1081 cgggcatccg gtccttcacc ggcgccggcc gcaccgtgac gtttaataat accgtcagtg
     1141 ccagcggaaa atatctcgcg ctgacggcat cgacgagcgg cacgatcacg atcggttcat
     1201 cggccaacgt cacgatcgcg gacctgtaca tcatgaacgg cggcacgctg aacttgggcg
     1261 gcagcacgct cacgaacgac agcggcaccg gcgttcggct gggcggtgat ttcggcacca
     1321 ccggcacgca ggatttgacc aagggcggga cgttcaacct gacggcgacc ggtggcggca
     1381 ccacctacgc gggcgtgatc aactcggtcg cgctgaacac ctcgggcgcg ctggcggtca
     1441 actcgaacaa caccagcggc gccaacacgc taagcgggca cgttgcgctg gactcggcgc
     1501 tgcagttcac ccaggccagc ggtggcacgc tgaacatcac gcaggcccgc gccggcggcg
     1561 tcggcacgac caccggcacc gacatcaagg ggcaaagcct cacgctgacc cccgccagcg
     1621 gcggcacgat caacttcagt ggcgacatct acaactccac cggcgccggc agcgtcctca
     1681 tgaacggcgc aggcactgtg acgttgggcg gagccaacac ctacactggt gcgacgacga
     1741 tcagcgccgg catcctcagc gttacagggg caatcaacag cgccaacgcc gccaacgtcg
     1801 ggcgaatctc cgtcgccaac acctcgggaa atgccgaact gctgatttcg ggcacggtaa
     1861 gagccaccaa cacaaccgcc ccgagcctct tagtcggcag cgtcagcggc gccaatggta
     1921 cgatcattgt caacatcggc ggcac
//
LOCUS       DATIMH010000118         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_608153, whole genome shotgun sequence.
ACCESSION   DATIMH010000118 DATIMH010000000
VERSION     DATIMH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608153"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..2206
                     /locus_tag="VLI90_01990"
     CDS             224..2206
                     /locus_tag="VLI90_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13003.1"
                     /translation="MSSNVEAAALSRVVGQSRRSIRTTAKAAAAGAGPLLALVMMGGG
                     VMPASVARAGTPQIVVYTGPNGGNWSLNSNWTPANTPSAGDTIFVTLAPTSAFSVNFD
                     SSYSDISGSRPAQLTINSSTTFPVTLNQVTALRNLFVGIETIGTTGQATYNQTGSTHG
                     PGTLFLGGNVGGSGTYLLSSAGVLSANSEVVGSAGAGTFSQSGGSNSTSGDLSIAQFA
                     NSAGTYSLTGTSSLSVGGSLYIGGSSGGAGGSGSFTIAGGTVSVPANIMRVYNTTGTQ
                     LNLSSGVLRTKALDLSGQPSLLNWTGGTLAFPAGYTVNSAFALGSTISMPGSGVLSND
                     AAAATNSGTINMNGGTLSGTGLLTNAGTINGPGTVAVPISNTGTISNAVGTLVVNANV
                     TSSGSATFAAGQQWATGTTFTNTAGIAGFQSDPGGATGAYNLNVNDTGGLVFFLGGTD
                     HLAGLSIASGVTAEIHAAAAPFHFPTIVDVTNLSFPGTGSGPGSGKLDVQVGELIVQS
                     SVATVQGMLANNNVTSTNLASNTALGYKDLNNGKTEVRFTVKGDANLDGLVNVGDLGA
                     LATNYGLSAGAVWSQGNFNNDAIVNVGDLGILATNYGMSLAGGPSAGGAVAADAAVAV
                     AAVPEPSMIAPMALLLSSIGARRFTRRKTLGPSGRR"
     gene            complement(2606..2677)
                     /locus_tag="VLI90_01995"
     tRNA            complement(2606..2677)
                     /locus_tag="VLI90_01995"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2643..2645),aa:Thr,seq:cgt)
     gene            complement(2850..3140)
                     /locus_tag="VLI90_02000"
     CDS             complement(2850..3140)
                     /locus_tag="VLI90_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13004.1"
                     /translation="MIQLNAGNVLLKPSQRRQILACLRRATKFGQRLGNFVLNLTLQR
                     TGRTYELRAQVRDRSGTFTCRARRNDWRDALREMTRTLLARLHGQWVTRSVA"
BASE COUNT          622 a         1104 c         1057 g          630 t
ORIGIN      
        1 tcactcgaaa tcaccctcac cccagcccgc gaggctgtgc ctcctcccac gagtacgcgg
       61 gagatgggga aaacgcgcag gctcgcttta accaccgcgc gctcgtcatc gcgtcaaaaa
      121 tccccttgag atcatccgcc aatcgcttta tgcttccgcc cacgcacgca ctcatgtgtt
      181 tctcatgcac ttcgtgagct gggatttgtt caagggagag gacatgtcat cgaacgttga
      241 agcggccgcg ctttctcgcg tcgtgggtca gagccgtcga tcgattcgca caaccgccaa
      301 agccgcggcg gccggggcgg ggccgttgct ggcgctggtg atgatgggcg gcggtgtgat
      361 gccggcgtcg gtcgctcgcg cggggacgcc gcagatcgtc gtgtacaccg ggccgaacgg
      421 cgggaactgg agcctcaact ccaactggac gcccgcgaac acgcccagcg ccggggacac
      481 gattttcgtc acgctcgcgc ccacgagcgc gttcagcgtg aacttcgaca gcagttattc
      541 cgacatctcc ggctcgaggc cggcgcagct cacgatcaac tcgtcaacca ccttcccggt
      601 gacgctgaac caggtgactg ccttgaggaa tcttttcgtc ggcatcgaga cgatcggcac
      661 aaccgggcag gcgacgtaca accagaccgg cagcacgcac ggccccggca cgctattcct
      721 cggcgggaac gtcggtggca gcggcacgta tctgctcagc agcgccggcg ttctgtcagc
      781 gaattctgaa gtcgtcggca gcgccggcgc cggcacgttt tcgcagagcg gcggatcgaa
      841 cagcacgagc ggcgatctta gcatcgcgca attcgcgaac tccgccggca cctattcgct
      901 caccggcacc agttcgttgt ccgtcggcgg cagcttgtac atcgggggca gcagcggagg
      961 cgccggtggc agcgggtcgt tcacaattgc cggcggcacc gtgtcggttc ccgcgaacat
     1021 catgcgcgtc tacaacacga caggaaccca gttgaacctt tccagcggtg tgctgcgaac
     1081 caaggcgctg gacctgtccg gtcaaccctc attgctgaat tggaccgggg gaacgctggc
     1141 ttttccggcc ggatataccg tcaacagcgc gttcgcgctg gggtcgacca tctcgatgcc
     1201 aggcagcggc gtactgtcga acgacgccgc cgccgccacc aacagcggca cgatcaacat
     1261 gaacggcggc acgctctcgg gcaccgggct gctcaccaac gccggcacga ttaacggacc
     1321 ggggacggtc gctgtgccga tctccaacac cggcacgatc agcaatgccg tcggcacgct
     1381 cgtggtcaat gcgaacgtga ccagcagcgg cagcgccacg ttcgccgcag gccaacagtg
     1441 ggcgaccggc accaccttca ccaacaccgc gggcatcgct ggcttccaat ccgaccccgg
     1501 tggcgcgacc ggcgcatata acctgaacgt caacgacacc ggcgggctgg tcttcttcct
     1561 cggcggcacc gatcacctgg cgggcctcag catcgcctcc ggcgtcacgg ccgagatcca
     1621 tgccgcggcc gcgccgttcc acttcccgac gatcgtcgat gtgaccaact tgtcgttccc
     1681 cggcaccggt agcggccccg gcagcgggaa gctggacgtg caggtcggcg agctgatcgt
     1741 gcagtcgtcg gtggccacgg tgcaaggcat gctcgccaac aacaacgtca cctcgacgaa
     1801 cctcgcaagc aacacagcac ttggctacaa agacttgaac aatggcaaga cagaagtgcg
     1861 cttcaccgtc aaaggcgacg cgaacctcga cggattggtc aacgtcggtg accttggcgc
     1921 gctggccacc aactacggcc tgagcgccgg cgccgtctgg tcccaaggca acttcaacaa
     1981 cgacgcgatc gtcaacgtcg gcgacctcgg gattctcgcc acgaactacg gcatgtccct
     2041 cgccggcggc ccgtccgcgg gtggagccgt tgccgcggac gccgctgtcg ccgttgccgc
     2101 tgtgccggaa ccgtcgatga ttgcgccgat ggcgctgctg ctgtcatcga ttggcgcgcg
     2161 gcgcttcact cgccgcaaaa cactcggccc ttcgggccgc cgctaaaagg gtgtctcgta
     2221 caaaggcgtg ttcgtcttaa cgatcaggaa aacgcgcatg cgacgcggct gccatggcca
     2281 tgtatgtgac ctgctggttc cgagcacttc ggttcctcgt tatgaccgaa ccaccagatc
     2341 ttcggctaac gctttcctgc accgcgaagc ccccagccga tcatcttctt ccagcccttg
     2401 gcgtggcgtg tgagcttgat ccgctcgtgc tccatcgttc ctccggtggc cgtcaatcgg
     2461 ccacgaggag gaaatcttaa actggttttt ggatatgcct ttgtttgcta ttggatacca
     2521 gcgtcaaaaa cgcagtaatc ggagcagcgg cgcgattcca aagaatcgag catatggcgt
     2581 ttggtcgtag cgatttaggg ggaaaagccg aaggagggat ttgaaccctc aacccccgct
     2641 ttacgaaagc ggtgctctgc cattgagcta cttcggcgcg gccgacggcc gttcgcacgg
     2701 cgtcgggctc caagtcgtgc cgaaggatat tgcgggcagg ggcgatttca aggggtacgg
     2761 tggcgcagag cgggcgcgac gcgcacgcac gcacgcacgc acttcccagt tacgcattgc
     2821 ggatgacgcg ttacgggttt ccccgcgcgt cacgccaccg agcgcgtcac ccattgcccg
     2881 tgcaggcggg cgagcagcgt gcgcgtcatt tcgcgcaagg catctcgcca atcattgcgg
     2941 cgggcgcggc aggtgaacgt gccgctgcga tcgcgcacct gggcccgcag ctcgtacgtg
     3001 cggccggtgc gttgcagcgt caggttcagg acgaagttcc ccaggcgttg gccgaacttg
     3061 gtcgcccgcc gaaggcaggc gagaatttgc cggcgttgtg atggcttgag caacacgttc
     3121 cccgcgttta gctgaatcat tgggcagact ccttgctcgc ggacggttca gtttaccaag
     3181 agcaatggtt gtaccggtga gaaatttttg acgattgtgg agagatttcg gcccgtgcgc
     3241 ttgagggggc gtcgaaacat tcgccgacgc cacgcatctt tcatttcccg atcgtgtgta
     3301 actgatcttt gtggtccggc gcttccttgg tcccctcgcc cggtactccg tcggtacgcc
     3361 gggagagggt tagggtgagg gtgatttggt ctggcgataa ccgctcgacg ttc
//
LOCUS       DATIMH010000119         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_319101, whole genome shotgun sequence.
ACCESSION   DATIMH010000119 DATIMH010000000
VERSION     DATIMH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319101"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VLI90_02005"
     CDS             <1..743
                     /locus_tag="VLI90_02005"
                     /inference="COORDINATES: protein motif:HMM:NF025802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HSV13005.1"
                     /translation="EMVFTSDGELPGDVEAIDLTQMAPVEWLDEGVVFYCKCGAPLWA
                     SAKYAGKRGVCRHCDVPVRVPKRKREKRAADAPRATDAAHAKCGVCHSQIIAGEETSA
                     CPDCGTTFHADCWTENYGCSTYGCPQVNALQPAKGEAVAEMTSPPAVMVEEREPGDAG
                     APWDLVLLASSVVGSLVGALLFGVPAIIVAIASLGRFISGKSRRRGLLALALILSLAG
                     VIGGLALSDFWWFSGRHLTPIRRMVHLT"
     gene            740..1369
                     /locus_tag="VLI90_02010"
     CDS             740..1369
                     /locus_tag="VLI90_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HSV13006.1"
                     /translation="MIAFSCGSCGKAFNVPETYAGRSARCKGCGAMVTVPSAPVAPLP
                     SPTPPPKVPMRIRRLRADAEEIASAFRKSTTICVTPAGGGEPPDMYRIEYRVDGLARG
                     PDGKPMPRDRHAVEIQLSSEYPRVSPKCKMLTPAFHPNIDAASICVGDHWTAGERLVD
                     LVIRIGEMIAYQEYNIRSPLDAEAAMWADLNAGKLPIDARDLRPMEDDV"
     gene            complement(1516..2808)
                     /locus_tag="VLI90_02015"
     CDS             complement(1516..2808)
                     /locus_tag="VLI90_02015"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSV13007.1"
                     /translation="MSQPPDPSSCADTRAAAVSAGQMQLVLNVSRMLAVTTDLDALLL
                     RIAESTCELMQCKRASIWLHDAEAKQLWTKVALGSGEIRVPDSAGIVGATFAANGVLH
                     VPQPYDDPRFNPANDKRTGFVTRSLLAAPAADSGGQPIGVIQAVNKRGPCFTPEDLTL
                     IQLLADQAGVAIQRHRLQMAALQAAEFRKEMDLARKVQEALLPSVIPKIPGFDAAGWA
                     KPASITGGDCYDFWQLPDGRLGIFLGDASGHGLAPTLVVSQARTLVRALCDSTQPACT
                     PHQILTRINSRLSEDLEPNRFITAFLAFLAPDGTLHWQSAGHGPIFFRPRAGEPMQSL
                     EAPVPPLNALPELMDDPPPPLQIDAGGALIVMSDGIFESFSPTKEQFGADRVIELLDR
                     SNSHPSKQCIDSLHQAVAKWQENDEPIDDQTIVIVKRS"
BASE COUNT          523 a          890 c         1052 g          513 t
ORIGIN      
        1 cggagatggt cttcacgagc gacggcgagt tgcccggcga cgtcgaagca atcgacctga
       61 cgcagatggc gcccgtggaa tggctcgatg agggcgtggt attttattgc aagtgtggag
      121 cgccgctgtg ggccagcgcg aagtacgccg gcaaacgcgg cgtgtgccgg cactgcgacg
      181 tgcccgtgcg tgtgccgaag cgcaagcggg agaagcgcgc cgccgatgcg ccgcgcgcga
      241 cggacgcggc gcacgcgaag tgcggcgtct gtcattcgca gatcatcgct ggtgaagaga
      301 cgagcgcgtg ccccgattgc gggacgacgt ttcacgcgga ctgctggacg gaaaattacg
      361 ggtgctcgac gtatggttgt ccgcaggtga atgcgctgca gcccgcgaag ggcgaagcgg
      421 tggccgaaat gacgtcgcca ccggcggtga tggtggaaga gagggaaccg ggcgacgcgg
      481 gcgcgccgtg ggatttggtg ctgctcgcca gcagcgtcgt cgggtcgctg gtcggcgcgt
      541 tgctgttcgg cgtccccgcc atcattgtcg ccatcgcgag cctcggtcgg ttcatcagcg
      601 gcaagtcgcg gcggcgcggg ctgcttgccc tggcgctgat cctctcgctc gccggcgtca
      661 tcggtgggtt ggcgctgagt gacttctggt ggttcagcgg ccggcatctg acgccgattc
      721 ggcggatggt tcacctgaca tgatcgcatt ctcctgcggc agctgcggca aagcgttcaa
      781 cgtccccgag acgtacgccg gtcgctccgc gcgctgcaaa ggctgcggcg cgatggtgac
      841 ggtgccgagc gccccggtcg cgccgctgcc atcaccaacg ccgccgccaa aggtgccgat
      901 gcgcatccgg cgactgcgcg ccgatgccga ggagatcgcg tcggcgtttc gcaagtcaac
      961 cacgatctgc gtcacccccg cgggcggcgg cgagccgccg gatatgtatc gcatcgagta
     1021 tcgcgtcgat gggctggcgc gcgggcccga cggcaagccg atgccgcgcg atcggcacgc
     1081 ggtcgagatc caactgagca gcgagtaccc gcgcgtgagt ccgaagtgca agatgttaac
     1141 gccggcgttt catcccaaca tcgacgccgc gtcgatctgc gtcggcgacc actggaccgc
     1201 gggcgagcgg ctggtggacc tggtcatccg catcggcgag atgatcgcgt atcaggagta
     1261 caacatccgc agcccgctgg atgccgaggc ggcgatgtgg gcggacttga acgcgggcaa
     1321 gttgccgatt gacgcgcgcg acttgagacc gatggaagat gatgtgtgag acaaccccat
     1381 ttagcccggc atgaacacac gccggtttcg ctgagaagtc gagaccgttc gatttctgaa
     1441 tcgacgggcc taaaagacgc ataaacccgg cgtgtgttca cgccgggcta aatgcggagt
     1501 tctacttcgt cgccgctatg agcgcttcac gatcacgatc gtctgatcat cgatgggctc
     1561 atcattctcc tgccacttcg ccaccgcctg gtgcaggctg tcgatgcact gcttcgatgg
     1621 atggctgttg ctccggtcga gcagctcgat cacgcgatcg gcgccgaact gttctttggt
     1681 ggggctgaac gactcgaaga tgccgtcgct catcacgatc agcgcgccgc ccgcgtcgat
     1741 ctgcagcggc gggggcggat cgtccatcaa ttcgggcagc gcgttcaacg gcggcacggg
     1801 ggcttcgagg ctctgcatcg gctcaccagc gcgggggcgg aagaagatgg ggccgtgacc
     1861 ggcgctttgc caatgcagcg tgccatcggg cgcgaggaag gcgaggaacg ccgtgatgaa
     1921 tcggttgggt tccagatctt cgctcaagcg cgaattgatg cgcgtcagga tctggtgcgg
     1981 cgtgcacgcc ggctgcgttg aatcacacag cgcgcgcacg agcgtgcgtg cttgtgagac
     2041 gaccagcgtg ggcgccagac cgtgacccga ggcatcgccg agaaagatgc ccagccggcc
     2101 gtcgggcagc tgccagaagt cgtagcaatc gcctccggtg atcgacgcgg gcttggccca
     2161 accggcggcg tcgaaaccgg ggattttggg aatcacgctg ggcagcagcg cttcctgcac
     2221 cttgcgggcc aggtccatct ccttgcgaaa ctccgcggcc tgcagggccg ccatctgcag
     2281 ccgatgtcgc tggatggcaa cgccggcctg gtcggcgagc agctggatca gcgtgaggtc
     2341 ttcgggcgtg aaacacggac cgcgcttgtt gacggcttgg atcacgccga tgggctgacc
     2401 gcccgaatcc gccgcggggg cggccaacag gctgcgcgtg acgaagccgg tgcgcttgtc
     2461 gttggcggga ttgaaccgcg ggtcgtcgta tggctgcggc acgtgcagca cgccgttggc
     2521 ggcgaaggtc gcgccgacga ttcccgccga gtccggcacg cggatttcgc ctgatcccag
     2581 ggccactttc gtccacagtt gtttcgcctc ggcatcgtgc agccagatgc tggcgcgttt
     2641 gcactgcatc aattcgcagg tggattcggc gatgcgcagc agcagcgcgt ccaggtcggt
     2701 ggtgaccgcc agcatgcgcg agacgttcag caccaattgc atctgccccg ccgaaacggc
     2761 ggcggcgcgc gtgtcagcgc aggaagatgg atcggggggc tgggacatgg agttgccgat
     2821 gcgtgcgcgt gcggtcgcgg cgcaggcaat cagaagtgta acaaaatgat aaacggcgag
     2881 atatgcggcg gacgctgcga atgacgaaat ccgaaatgcg aattcccaaa tgaatgacga
     2941 atgacgaaag tgcgagtgac gaatggatgc aggaaaat
//
LOCUS       DATIMH010000120         5179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4360622, whole genome shotgun sequence.
ACCESSION   DATIMH010000120 DATIMH010000000
VERSION     DATIMH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4360622"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1351)
                     /locus_tag="VLI90_02020"
     CDS             complement(122..1351)
                     /locus_tag="VLI90_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSV13008.1"
                     /translation="MTVALDPAQTSAVSQKLAPAMRLLFWETTTGCNLACIHCRRLEV
                     SLALSKLDMSTEQAKAMIRSLPETGRPILVFSGGEPLMRPDIFELAAFANEVGLPTAL
                     ATNGTMIDEAKAKQIVAAGFRRVSISFDGPDAPTHDQFRGDGAFESSVQGFKNLRKLG
                     MSMQINTTIARHNHKKLDETYRLALELEADALHTFMLVPVGCGMDLADDVWLNGDDYE
                     TALNWIYDRSLEGKLHLKATCSPHYFRVMRQRAKADGRPMPAAAHPHRGMHPGAAPRG
                     APHVHAGPQGLGHPAGHPGGHPGGANANMTAMTKGCLAGQAVCFVSHTGEVFPCGYLP
                     LTSGNVKTTPLPQIWRESKIFADLRGDAELEGKCGVCEYSKVCMGCRARAYGHTKNYL
                     AEEPNCDFIPLRVQRKQ"
     gene            complement(1591..1992)
                     /locus_tag="VLI90_02025"
     CDS             complement(1591..1992)
                     /locus_tag="VLI90_02025"
                     /inference="COORDINATES: protein motif:HMM:NF007714.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HSV13009.1"
                     /translation="MNPPTTAPGSATVANEHKQRVMARDLRTLAVFIDVYCRHKHRDA
                     IRQRVSLNTHDAERIAGRELHLCEDCRKLLAHAFVKRSHCPMDPKPMCKHCPSHCYHP
                     TYRAKMQEVMRFAGKRSVLSGRLDFLFHLFF"
     gene            2100..3062
                     /locus_tag="VLI90_02030"
     CDS             2100..3062
                     /locus_tag="VLI90_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HSV13010.1"
                     /translation="MPARIAILVSVLGLTAAVILWVERPQPTQADVARAPANEPTAGK
                     APLRLGLVPERDIFAQRRHYRTLAEYLARRLDQPVELVTLGSYEQILSDFADHRVDAA
                     FLGSFVTVLAVDRLGAQVLVKPAAADGKSTYHGVVFVRGDSPARSLSDLAGKRVVMVR
                     ATSAGSLFPLCQLRRSGLLEGPNAVKIRWVGTHDDAVQEVIDGAADAGAAKDSRLDQM
                     QIEQPAIRLRRLATSDAVPENALVVRSDLSSRWGPRLTDVLSQMSSNDEGRAVLRDFG
                     ADRFAPSNIDEYRPVYQMCGDVNDRCWTMQGIPGAPPRWPTTSR"
     gene            3086..4165
                     /locus_tag="VLI90_02035"
     CDS             3086..4165
                     /locus_tag="VLI90_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HSV13011.1"
                     /translation="MLRSRLMMIMGPVVALLVGSVVAAVWLLQGVLRDMRHVDTQAWV
                     VVERTNAFSTAVNSIEAELYRLQLHRDSHVDRLIDCVEEAHGLLDGVGESYVVHLPAA
                     LPWFDGLNRLMPDFDRHVSALATAQDPQLAAEHLEAGLTAAAQLREQTLPLSRFIQGH
                     ARQEQNALIHHFRVLVLGISLAFLLVINLSILALLRMATMILRPVDALLEGAKQLGRE
                     RFDYRIDLAGNDEFGQLALAYNRMAEQLQANEQRKMDTLAQVGLTMNHELNNSISIIQ
                     LQLRLIDRRIGGNDAAQAKSLMQIRDSLSRMASTVEALKHVRRIVLTDYTPGTKMLDL
                     RRSVDADEPPTPAMDAVGANHGSVK"
     gene            4162..>5179
                     /locus_tag="VLI90_02040"
     CDS             4162..>5179
                     /locus_tag="VLI90_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSV13012.1"
                     /translation="MTRQQRIGRMPLYRQLHWFTRLRWLAGGTVLAFGGADLLLFHWH
                     HAWEAERFAIVGAVVLLYNAALAALLRHRRRQPAPQTWPRHVALLQILLDLACLTPIV
                     LWTGGLTSAILGFFVFHMVFASLLLAPAAAYGTACAAVLMLTIGLIQNNQLPRQHPAN
                     LALPGIACTLLLTVMLTNHVTRDLRRHQLRLVRQGRRVRLLGARLRQHQRAMAQQEKM
                     VALGQIAAGVAHEIANPLASMDSLLQLVQRKPERWGPEKLTTLREQISRINQTIQQMR
                     RFAHPAEGNLESVPLNDVADDAIRMVEFDRRVKELTIVRQFDSDVEGALVMPQAIQQV
                     LVNLM"
BASE COUNT          905 a         1617 c         1699 g          958 t
ORIGIN      
        1 gcgcgcacga tcacgaactg ccccggaccg caatggcgcg tgacgtgtgg cgtttcaacc
       61 accagccggc gcaccgccgg cgctagccag tcggattgga tgacgcggta tcccatgggg
      121 ctcactgctt tcgctgcacg cgcagcggga tgaaatcgca attcggttct tccgccaggt
      181 aatttttcgt atggccatac gcgcgggcgc ggcagcccat gcagaccttg ctgtattcgc
      241 acacgccgca cttgccttcc aactcggcgt cgccgcgcaa gtcggcgaag attttgctct
      301 cgcgccaaat ctgcggcagc ggcgtggtct tcacgttgcc ggatgtcagc gggagatacc
      361 cgcatggaaa aacttcgccg gtgtgtgaga cgaagcagac agcctgaccc gcaagacaac
      421 ccttggtcat cgcggtcatg ttcgcgttgg cgccgccggg atggccgccg ggatgaccag
      481 cgggatgtcc aagaccctga ggccccgcat ggacatgcgg ggccccgcgt ggcgctgcgc
      541 caggatgcat cccacgatgc ggatgcgccg ccgcgggcat cggccgacca tcggcctttg
      601 cacgctgccg catcacgcgg aaatagtggg gcgaacacgt tgccttcagg tgcagcttgc
      661 cttcgagact gcggtcgtag atccagttca gcgcggtttc gtaatcgtcg ccgttcagcc
      721 agacgtcgtc ggccagatcc attccgcagc ccacgggaac gagcatgaac gtgtgcagcg
      781 cgtccgcctc cagctccagc gccaagcggt atgtttcgtc gagcttcttg tgattgtgcc
      841 gggcgatcgt cgtgttgatc tgcatcgaca tgccgagctt gcggaggttc ttgaacccct
      901 gcacggacga ctcaaacgcg ccgtcgccgc ggaactgatc gtgcgtcggc gcatcgggcc
      961 cgtcgaagct gatcgatacg cggcgaaaac ccgcggccac gatctgcttg gctttcgctt
     1021 cgtcgatcat cgtgccgttg gtcgccagcg ccgtgggcag gccgacttca ttcgcgaacg
     1081 cggccaattc gaagatgtcc ggccgcatga gcggctcacc gccggagaag accaggatcg
     1141 gccggccggt ttcgggcagc gagcggatca tcgccttcgc ctgttcggtg ctcatgtcca
     1201 gcttgctcag ggcaagcgac acttccaaac ggcggcagtg gatgcacgcg aggttgcagc
     1261 cagtggtggt ttcccagaac agcaagcgca tcgccggcgc gagtttttgt gaaaccgcgg
     1321 aagtttgggc gggatcgagg gcaacggtca ttaggtcatc tcctgtgggc gcgggcgcgg
     1381 ccgcaccgcc agaggaccaa tgaacaacag cgatgccagc ggagaatcac gcgagaactg
     1441 ggtgttttgc aggaagcgcg gagatcactt ctcaacggcg ggaggcgaaa ggatgagggt
     1501 tttcttacag ggagagccgg gccatcgtcg gcccggactt tatcaatcga gcgcagaccg
     1561 ggccgaggac ggcccggctc tccttgcgga tcagaagaac aaatgaaaca ggaagtccaa
     1621 ccgcccgctc aagaccgatc gtttccccgc gaagcgcatt acttcctgca tcttcgcccg
     1681 ataagtcggg tgatagcagt gcgacgggca atgtttgcac atcggtttcg gatccatcgg
     1741 acaatgcgat cgtttaacga acgcgtgggc cagcagcttt cggcagtctt cgcacaaatg
     1801 cagctcccgc ccggcgatcc gctcggcgtc gtgcgtgttc aatgacacgc gctgccggat
     1861 ggcgtcccgg tgcttgtgcc gacaatacac gtcgatgaac accgcgagcg tgcggaggtc
     1921 gcgggccatc acgcgctgct tgtgttcgtt ggcaacggtg gcagaaccag gggcggttgt
     1981 gggcgggttc atgcctcagg ttctgcacga tccgtgccgc tcgcgggctg gtctgccgtt
     2041 tgttctgaag tcatcacacg cggccgcgga accccggttg gaaagcgatt ttaagcttgt
     2101 tgcctgcgcg tattgccatt ctcgtctcag tcctgggatt aaccgccgcc gtgattctct
     2161 gggttgagag accccagccc acgcaggcgg acgtcgcccg tgcgcccgcc aacgaaccga
     2221 ctgccgggaa ggcaccgctt cggctggggc tcgtgcccga gcgagacatc ttcgcccagc
     2281 gccgccatta tcggacattg gcggagtacc tggctcgccg cctcgatcaa ccggtcgaac
     2341 tggtgacgct tgggtcttac gagcagatcc tcagcgactt cgccgaccac cgtgtggacg
     2401 ctgcctttct cgggtcgttc gtcacggtgc tggcggtcga tcggctcggg gcgcaggtgc
     2461 tggtcaagcc cgcggcggcc gacggcaaaa gcacctacca cggcgtcgtc ttcgtgcgcg
     2521 gcgattcgcc cgcgcggtcg ctctccgacc tggccggcaa gcgggtcgtc atggttcgcg
     2581 ccacttctgc cggaagtttg ttcccgctgt gtcagttgcg gcgctcggga ctattggaag
     2641 gtccaaatgc ggtgaagatt agatgggtgg gtacgcacga tgatgcggtg caggaagtga
     2701 tcgacggcgc cgccgacgcc ggggccgcca aagattcgcg gctggatcag atgcagatcg
     2761 aacaaccggc gatccgcctg cggcgattgg cgaccagcga cgccgtgccg gaaaacgcgc
     2821 tggtcgttcg cagcgatcta tcgtcgaggt ggggaccgcg gctgacggac gtgttgagcc
     2881 agatgtcgag caacgatgaa ggccgcgcgg ttcttcgtga cttcggcgcc gatcgcttcg
     2941 cgccgtcgaa catcgatgaa taccggccgg tctaccaaat gtgcggcgac gtgaacgacc
     3001 gctgctggac gatgcaagga attcccggcg cgccaccgcg gtggccgaca acgtcgcggt
     3061 gattgcgagc ggcaagagtg attttatgct tcgaagtcgt ttgatgatga tcatgggtcc
     3121 ggtggtggcg ctgctggttg gaagcgtcgt cgccgcggtc tggctgctgc agggtgtgct
     3181 gcgcgacatg cggcatgtcg acacgcaggc atgggttgtc gtcgaacgca ccaacgcctt
     3241 cagcaccgcc gtcaattcca tcgaggccga gctctatcgc ctgcaactgc atcgcgacag
     3301 ccacgtcgat cgcctgatcg attgcgtgga ggaggcgcac ggcctgcttg acggtgtagg
     3361 tgaatcgtat gtcgtgcatc tgccggcggc cctgccgtgg ttcgatggac tgaaccgcct
     3421 gatgccggat ttcgatcgcc atgtcagcgc gctggcgacg gcgcaggatc cacagcttgc
     3481 cgcggagcat ctggaagccg ggctgaccgc ggccgcgcag ctgcgcgagc agaccttgcc
     3541 gctgagccgg ttcatccagg gccacgcgcg gcaggaacag aacgcgctga tccatcattt
     3601 ccgggtgctg gtgctcggca tttcgctggc attcctgctg gtgatcaacc tgtcgatcct
     3661 ggcgctgctt cgcatggcga cgatgatcct gcgtccggtc gatgcgctgc tcgagggcgc
     3721 caaacagctg ggccgcgagc gcttcgacta ccgcatcgac ctggccggca acgacgaatt
     3781 tggccaactg gcgctggcgt acaaccgcat ggccgagcag ctgcaggcca acgagcagcg
     3841 caagatggac acgctggcgc aggtcgggct gacgatgaac catgagctca acaactcgat
     3901 cagcatcatc caactgcagc tgcgcctgat cgaccgccgg atcggcggca acgacgcggc
     3961 acaagccaaa tcgctgatgc agattcgcga cagcctcagt cggatggctt cgacggttga
     4021 agcgctcaag cacgttcgcc gcatcgtcct cacggattac acgccgggaa cgaagatgct
     4081 cgatttgcgc aggagcgttg atgctgacga accgccaaca ccggcgatgg atgccgtggg
     4141 tgccaaccac gggagcgtga aatgacccgc caacagcgca ttggccgaat gccgctctat
     4201 cgccagctgc actggttcac ccgcctgcgc tggttggccg gtgggacggt gctggcgttt
     4261 ggcggcgccg atctgctgct gttccactgg catcacgcgt gggaagccga gcgctttgcg
     4321 attgtcggcg cggtcgtgct gctctataac gcggcgctgg cggcattgct gcggcatcgc
     4381 cggcggcaac cggcgccgca aacttggcct cgacacgtcg cgctgctgca gatcctgctc
     4441 gacctggctt gcctcacgcc gatcgtcctg tggaccggtg ggttgaccag cgcgatcctc
     4501 ggtttcttcg tcttccacat ggtcttcgcc agcctgttgc tggcgcccgc cgcggcgtac
     4561 ggcacggcgt gcgcggcggt gctgatgctc accatcggtc tgatccagaa caaccagttg
     4621 ccgcggcaac atccggcgaa cctcgcgctg ccgggcatcg cctgcacgct gctgctgacc
     4681 gttatgttga ccaaccacgt gacgcgcgat ttgcgccgac atcagctgcg gctggtccgc
     4741 cagggccgcc gcgtgcgctt gctcggcgcg cgcctgcggc aacatcagcg cgcgatggcg
     4801 cagcaggaga agatggtcgc cctcgggcag atcgccgcgg gcgtcgcgca tgaaattgcg
     4861 aaccccctgg ccagcatgga cagcctgctg cagctggtgc agcgcaagcc cgagcgctgg
     4921 gggccggaaa agctgacgac gctgcgcgaa cagatcagtc gaatcaatca gacgatccag
     4981 cagatgcggc gatttgccca tcctgccgaa ggaaacctcg agtcggtccc gctcaacgat
     5041 gtcgccgatg acgccatccg catggtcgag ttcgaccgcc gggtgaagga gctgacgatc
     5101 gtgcggcagt tcgacagcga cgtcgagggc gcgctggtca tgccgcaggc gattcagcag
     5161 gtgctggtca atctcatga
//
LOCUS       DATIMH010000121         5868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2339430, whole genome shotgun sequence.
ACCESSION   DATIMH010000121 DATIMH010000000
VERSION     DATIMH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339430"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VLI90_02045"
     CDS             <1..192
                     /locus_tag="VLI90_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13013.1"
                     /translation="DQLSAISGTTITYRSDGRVTSTPAVIVVATSNPDVQARCIRIDL
                     SGRPNVNEYAFGGGTNTCS"
     gene            204..878
                     /gene="pilV"
                     /locus_tag="VLI90_02050"
     CDS             204..878
                     /gene="pilV"
                     /locus_tag="VLI90_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HSV13014.1"
                     /translation="MPRRFSNSPGCAGLATSRVLGFTMIEVLVTLVVLALGLLGVMGL
                     QARGQQAELESYQRGQALVLLQDMVDRISANRTDAHSKLYSTVTTFPVGGGGALTNCG
                     VSTTGAAYDVCDWGNQLNGAAEASASGTCDTSAGSGCIGAMLGARGCITYDNSTELKD
                     STGATMAGTGIYTVTVAWQGLVATAAPPTAVTCGRNLYPNATSPNDAMRRAVTTTIRI
                     ANLNSP"
     gene            933..2033
                     /locus_tag="VLI90_02055"
     CDS             933..2033
                     /locus_tag="VLI90_02055"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HSV13015.1"
                     /translation="MNKRFLSRLSGGFSLIELMVALTLAGILLLGLSVFFVSSSRSYS
                     EAEHVSRQIENGRYASALIAEEARHAGFYGEVKNVSLLPTSPLPSSLIPVPGTLPDPC
                     ATAIASVKAALPVPIQGIDSPDAAPSCVTDALAGTDMIVVRRANTTTVTAAAADAIAG
                     GYYTQTANCATGAPVFQVALSGASPTNLGLTDRNCTTAMPVRQYHVYIYYVAPCSIAT
                     GTNGTCKPTDKALPTLKRVELCSVPATTNPACSGGAGTMSDPEPLVEGIENLQIEYGL
                     DTSGDGEADTFTAKPTTVAQWVQVVAVRFHILARNTDVSPGFTDTKTYALGKNSDGTT
                     NTVTPGGAYRRHNYTELVRVTNVSQRLERTFP"
     gene            2030..2593
                     /locus_tag="VLI90_02060"
     CDS             2030..2593
                     /locus_tag="VLI90_02060"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HSV13016.1"
                     /translation="MNRIAFRSRTSQRGATLVIGMIMLVLVTLFVLAAINMSTTNLRV
                     MGNEQARNEAVAAAQQAVEQVSTVDFTKNPPTTTTPTVVAVDINGDGTSDYQASVSAA
                     CLNSVPVKVTELNPADVVNDGPCFLSTSSSTSGIAGGSSSGDSLCSATQWDVAAAVSD
                     ATANTTSVAVTLHQGVSKRIATGDTPC"
     gene            2647..>5868
                     /locus_tag="VLI90_02065"
     CDS             2647..>5868
                     /locus_tag="VLI90_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13017.1"
                     /translation="MKKTITACAMLAALASVISTSHAEDIDIYGNNGGGPKPNIIIVF
                     DNASRNNSNFSGTCPYAGITNISTLQDATRCALAVAVSDIASQPALLGKFNMGLLVYG
                     YQDNQGGQWIIPSITPGAANVATPLLTMDQTGINTFIAAVTPPINTANNASSSGGMQE
                     AWAFFTGSPAAATEVFGSKTTYVSHETQSCQKSFIIFIGAATGNNGEPETGNSGASGG
                     AAGLANAGATAAQQVQINTSTIGTNTGDDSAWGDEWARFLNQTDFSGSAANSQNIVTY
                     TIATGGTRPNYVQFLQSMANQGGGKAFVAADINTMVQALLQIFNEIQSVNSVFASSSL
                     PISSNSQGTYLNQIFIGMFRPDGSANPRWMGNLKQYQFGVGGTPALPTLFLADKNGSS
                     AISGAGTGFISPTAVSFWNPAIDSSKLPDSIGGFWVNNPQSAGGGYDNPDGEIVEKGG
                     VAQQIRLANLQDDYTANPSSPRKMYTCTGTTGSCVSGDSLSATPFATTNASLTDASFG
                     ITPPTTTISSFSRSGATVTAVLSAAPTPALTNGQSVTVQGASSPELNGTFSIGLVNST
                     TFTYTISVSPPSPATGTYTASIPSSPKTITALSRVGTTVTATSAAHNYNVGDSVTITG
                     ATGSEYNGTFSILSVTTNTFTYKISDSPGAATTGGTADLGTTHTNGLTFTSTQITRDT
                     THSDNTSIVTVTASGNVNSGYKAGAKVNISGVSPTSYNVTLATIIAGGGNNSGDTCPG
                     GSKQKSFCYSITTTPATPDAGGSPKVDRTATTALSGLTRTLACTGSSPSTATTVTATV
                     TSSNPFKAGDVVSIAGAPVGANEAAYVGSFTVLSATNTSGGGETFTYSTTTAPSCTPS
                     ATGASAITSSTGVDKNDLINWVRGYDSVGDEASPGSGITIRPSVHGDVLHSRPVVVNY
                     GGSIGVVVFYGANDGTFRAINGNQPLNGSTAVGGASPGGEIWSFIPTEFFSKLVRLYQ
                     DSPSVKLASTPDGITPTPLRKDYFFDGSTGAYQASDGTVTLFLSERRGGRFIYALNVT
                     DPTNPILLWKKSNLDSNMGELGLTWSTPKPALVRGYSNQVVIFG"
BASE COUNT         1246 a         1788 c         1728 g         1106 t
ORIGIN      
        1 gatcaactgt ccgccatatc ggggaccacg atcacgtatc gaagcgatgg ccgtgttacc
       61 tcgacgccgg cggtgatcgt cgtcgcgacg agcaatcccg acgtgcaggc gcgctgtatc
      121 aggatcgacc tgagcggacg cccgaatgtc aacgaatacg cttttggcgg cgggaccaac
      181 acatgcagct gacaaaaaca gccatgccga gaaggttttc gaattctccg ggatgcgcgg
      241 gccttgcaac atcgcgagtc ctcggattca cgatgatcga ggtccttgtc acgcttgtcg
      301 tgctcgcctt gggattgctc ggcgtgatgg gtcttcaggc gcgcggtcag caggcggagc
      361 tcgagtcgta tcagcgtggt caggcgcttg tcctgctgca ggacatggtg gaccgcatca
      421 gcgccaatcg aactgatgcc cacagcaagt tgtactcgac ggtaaccaca ttcccagtgg
      481 ggggtggcgg tgctctcacc aattgcggag tatcgaccac cggtgcagct tacgacgttt
      541 gcgactgggg aaatcagctc aacggcgccg ccgaggcatc ggcgagcggc acgtgtgata
      601 cgtccgccgg gtcgggctgc atcggcgcga tgctgggtgc gcgcggctgt atcacttacg
      661 acaactccac ggagctaaag gatagtaccg gcgcgacgat ggccggaacc ggaatctaca
      721 cggtcaccgt ggcatggcag ggacttgttg cgacggcggc tccgccgacg gccgtcacct
      781 gcggacgcaa cctttatccg aacgctacgt cgccgaatga cgcgatgcgc cgtgctgtta
      841 ccaccaccat tcgcatcgct aatctgaatt cgccgtaggg catcaccaaa atgtctactc
      901 ggatttgtac ctcatttccc gcgcgctccg gaatgaacaa gcgattcttg tcgcgccttt
      961 ccggcggctt ctcgctgatc gagttgatgg tcgcgcttac gctcgccggc atattgctgc
     1021 tcgggctctc ggttttcttc gtttcaagca gtcgttcgta ctcggaagcc gagcatgtga
     1081 gccgccagat cgagaacggg cggtacgcct ccgcgctcat cgccgaggag gcccgtcatg
     1141 ccggcttcta tggcgaggtc aagaacgtct cactgctccc gacatcgccc ctgccgtcat
     1201 cgttgatccc cgtgccgggc accttgcctg atccgtgcgc taccgcgatc gcgagcgtga
     1261 aagcagcact gccggtccct atacagggca ttgattcacc ggatgcagcg ccaagctgcg
     1321 tgaccgacgc tctggccggg actgacatga tcgtcgtccg tcgtgcaaat acgacgacgg
     1381 tcacggccgc ggctgccgac gctatcgccg gagggtatta cacgcagacg gcaaactgcg
     1441 cgacgggcgc gccggtattc caggtggccc tgagcggcgc gtcgccaacc aaccttgggc
     1501 tgacggaccg caattgcacg acggcaatgc cggtccgcca gtatcacgtc tacatctact
     1561 acgttgcccc gtgcagcatc gcgacgggga cgaacgggac ctgcaagccg accgacaagg
     1621 cgctgccaac gttgaagcgc gtggaattgt gcagtgtgcc cgcgacaacg aatccggcct
     1681 gctctggcgg tgccgggacg atgtccgatc cggaacctct cgtcgagggc attgagaacc
     1741 tgcagatcga atacggattg gacacttccg gcgacggcga ggccgatacg ttcacggcga
     1801 aaccgacgac ggttgcgcaa tgggttcagg tggtcgcggt tcgcttccac attcttgcgc
     1861 gcaacaccga cgtcagccca ggatttacgg atacgaagac gtacgcattg ggcaagaact
     1921 cggacgggac cacgaatacg gttactcccg gtggcgcata ccgtcgccac aactacaccg
     1981 aactggttcg cgtcaccaac gttagccagc gcctcgaaag aaccttccca tgaatcgcat
     2041 tgcattccgt tcccgcacct ctcagcgcgg ggcaacgctc gtcatcggca tgatcatgct
     2101 ggtgctggta acacttttcg tgcttgcggc catcaacatg tcgacgacta acctgcgtgt
     2161 tatgggcaat gagcaggcac gaaatgaggc ggttgccgcc gcgcaacagg cggttgagca
     2221 ggtgtcgacg gtcgacttca ccaagaatcc tccgacgacc acgaccccga cggtggtagc
     2281 cgtggacatc aacggagacg ggacatccga ttaccaggcg tcggtatcgg ccgcctgcct
     2341 caattcggtt ccggtcaagg taacggagct caatcccgcc gacgtcgtca acgacggccc
     2401 atgctttctg tcgacgtcgt ccagtacgtc gggcatcgcc ggcggcagtt cgagtggcga
     2461 cagcctgtgc tcggccacgc aatgggatgt ggctgccgcg gtttccgatg cgacggccaa
     2521 caccaccagc gtcgcggtga cattgcatca aggggtcagc aagcgaattg cgacgggtga
     2581 taccccgtgc taagcctcga cgcgaaccag cgcccggggc gtgcagcaag aagggaacat
     2641 aagaccatga aaaaaacgat cacagcttgc gcgatgctag cggcgcttgc gagtgtcatt
     2701 tcgacctcgc atgcggaaga catcgacatc tatggcaaca atggtggggg tccgaagccc
     2761 aatatcatca tcgtcttcga caatgcgtcg cgcaacaaca gcaacttctc cggaacgtgt
     2821 ccgtatgcgg gtatcacgaa tatttcaacg ctccaggacg cgacccggtg tgcgcttgcc
     2881 gtggcggtca gtgatatcgc gagccagcct gcccttctcg gcaaattcaa catggggcta
     2941 ctggtatatg gctaccagga caaccagggc ggccaatgga tcatcccgag catcactcca
     3001 ggagctgcga acgtcgccac cccactcctc acgatggatc aaacgggaat caacacattc
     3061 atcgccgccg taacgccgcc gataaacact gccaataacg cgtcgtcaag tggtggcatg
     3121 caggaagcgt gggctttttt caccggtagc ccggccgcgg caaccgaagt cttcggatcg
     3181 aaaacgacgt acgtgtcgca tgaaacacag agctgccaga agagcttcat cattttcatc
     3241 ggcgccgcga ctggcaacaa cggcgagccg gaaaccggta actcgggcgc gtcgggcggc
     3301 gcggcgggct tggcaaacgc gggcgctacc gcagcacagc aggttcaaat caatacgtcg
     3361 acgattggca cgaataccgg cgacgactcc gcttggggcg acgaatgggc gcgcttcctg
     3421 aaccagaccg atttcagcgg atcggcggcc aattcacaga acatcgtgac ctacacgatt
     3481 gcgaccggcg gaacccggcc gaattatgtt cagttcctgc aaagcatggc gaaccagggt
     3541 ggaggcaagg cgttcgtcgc tgcggacatc aatacgatgg ttcaggcgct gctgcagatc
     3601 ttcaacgaga tccaatcggt caacagtgtg ttcgcgtcat ccagcctgcc catcagctcg
     3661 aatagccagg gaacgtatct aaatcagatc ttcatcggca tgtttcgtcc ggacggcagc
     3721 gccaaccccc gatggatggg aaatctcaag cagtaccagt tcggcgttgg cggtacgcct
     3781 gcattgccca cgctttttct cgccgacaag aacggaagct ctgcgatcag cggagcgggt
     3841 accggattca tctcgcccac tgcggtgagc ttctggaatc cggcgattga ctccagcaag
     3901 ttgcccgatt cgatcggtgg cttctgggtg aacaacccgc agagcgccgg cggcggttac
     3961 gacaaccccg acggcgaaat cgtcgagaag ggcggcgttg ctcaacagat ccgcctcgcg
     4021 aatctgcagg acgactacac ggcgaatccg tcctcgcccc gcaagatgta tacatgtacg
     4081 ggaaccaccg gttcatgcgt gagcggcgat agtctctctg cgacgccgtt cgcgacaacg
     4141 aatgcgtccc ttacggatgc gtccttcgga attaccccac cgacaacgac catttcatca
     4201 ttcagccggt cgggagcgac ggttactgcc gtactctctg ctgcgcctac gcccgcactt
     4261 acgaacggtc aatcggtcac ggtccagggt gcgagctcgc ccgaactgaa cggaaccttc
     4321 agcatagggc tcgtcaattc gacgaccttt acctacacga tctcggtaag tcccccttca
     4381 cctgccacgg gcacgtatac ggcaagcatc ccttcgagcc caaagacgat caccgcactt
     4441 tcccgcgtcg ggacgaccgt gacggccacg agtgccgcgc acaactacaa cgtcggcgat
     4501 tcggtgacga tcaccggggc gacggggtcc gagtacaacg gaacgttctc gatcttatcg
     4561 gtaaccacca acacgttcac ctacaagatc agtgatagcc ccggcgcggc aacgaccggc
     4621 ggaaccgccg atttgggcac gacccatacg aacgggctca cgttcacatc gacacagata
     4681 acccgcgaca cgacgcacag cgacaacact tcaattgtca cggtgaccgc ctccggtaat
     4741 gtgaacagcg gctataaagc gggcgcgaag gtgaacattt ctggcgtctc gccgacaagc
     4801 tataacgtaa cgctcgcgac gatcatcgca gggggaggga acaactcggg cgatacgtgt
     4861 ccagggggca gcaagcaaaa gagtttctgc tattcgatca ccactacccc ggcaacgcca
     4921 gacgcaggtg gctctcccaa agtagaccgc accgcgacga ccgcactttc cggtctcacg
     4981 cgaactctcg catgtaccgg aagttcgccg tcgaccgcga cgacagttac ggcgacggtt
     5041 acctcgtcga acccgttcaa ggcgggagat gtagtaagca ttgccggtgc gccggtcggc
     5101 gccaacgaag cggcatacgt tggatcattt acagtgctta gtgcgaccaa tacaagtggc
     5161 ggtggcgaga cgttcacgta ttcgacgaca acagcgccat cttgtactcc gagtgcgact
     5221 ggcgcgtcgg cgatcacgag ctccacgggt gttgacaaga acgatctgat caactgggtg
     5281 cgcggctacg atagcgttgg tgacgaagcc agcccgggca gtggcataac gatccgtcct
     5341 tcggtgcacg gggatgtgct ccattcgcgg cccgtcgttg tgaattacgg cggttccatc
     5401 ggtgtcgtcg tgttctatgg cgccaacgac ggcacgttcc gtgcaatcaa cggtaatcag
     5461 ccactcaacg gctctacggc cgtaggcggc gcttcgcccg gcggggagat atggagcttc
     5521 atcccgacgg agtttttcag caaactcgtt cgtctctatc aggacagtcc atcagtaaag
     5581 ctggcaagta cgcccgatgg aattacgccc accccattga gaaaggatta tttcttcgat
     5641 ggctcgactg gagcgtatca agcgtcggac gggacggtaa cgcttttcct gagtgagcga
     5701 cgcggggggc gcttcatcta tgcgctgaat gtcaccgacc cgacgaaccc gattctgctc
     5761 tggaagaagt cgaatctgga ttcgaacatg ggcgagttgg gacttacgtg gtctacgccg
     5821 aagccggcgc tcgttcgtgg gtattccaat caggtggtga ttttcggc
//
LOCUS       DATIMH010000122         1959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_896473, whole genome shotgun sequence.
ACCESSION   DATIMH010000122 DATIMH010000000
VERSION     DATIMH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896473"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..>1959)
                     /locus_tag="VLI90_02070"
     CDS             complement(198..>1959)
                     /locus_tag="VLI90_02070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV13018.1"
                     /translation="NAWQLDMTLSSASPSHLMTSAHGALPPATPVNAKHLIALCALCL
                     AMLLPFLAKPVHNDDPVFIWPARQITRHPLDFYGGLINWEWREQPMSEVLQNPPLFSY
                     DLAGMSAIVGWSEIALHAAALPFGLLTVVGTYLLATQFTRRPALAALLLLCCPAFLVS
                     ATTLMCDVPLLCFWVWSVLFWVWGTRRSAMWLPLAGLSTAAAGLTKYPGINLLPLLIA
                     GAALWPANRRTRLIQIASLLIPIAAWAGYEWFTRIHYGNGLLLGAASHVKVTQSGITI
                     PPLLGILDSLAFIGGGVFAVAVLAVLLAPPRLRGFALPPLVILLGVVASRCVAPPPAW
                     SGAQPIGPAWVFFLQYGLLAAAGVAIFCLCGSFMSQRLRRTGWRDDVFLLLWIGGVAV
                     FATFLNWAINARTILPLVPAACILGARAVDRFGAPSARWLAIALAPAAALAICVAVAD
                     YRMASICRDVVDRLLSVPAVAEAYSQGRLWFAGHSGFQYYMDRRGVRAVDAEHPQFKP
                     GDLVIFPLNSYGATPGGQGFHFVGLYQDPTERWLATMHAPMGGGFYTSFGQALPFVFG
                     PVPPESYSILQVTPGTRLYH"
BASE COUNT          414 a          631 c          649 g          265 t
ORIGIN      
        1 tcgtgttcgt ccgccagcag atacacgacc cacttcgccg ggaccgtttt caaaaacgcg
       61 tcgaaatccc cggcgggatc gaagtcggcg tggtggtcga atggcaggtc gtcgtcgaga
      121 agcatcggca cactcgatga tatgaaccaa acccgcggct gtcgcccttc tcgcgcccgc
      181 cacgtgccgt gcgcatctta atgatacaga cgcgtgcccg gtgtcacctg aagaatgctg
      241 tacgactccg gcgggacggg cccgaacacg aacggcaagg cttggccgaa cgacgtgtaa
      301 aaaccgccgc ccatcggggc gtgcatggtg gcgagccatc gctcggtcgg atcttgatac
      361 agacccacga agtgaaagcc ctgcccaccc ggcgtggcgc cgtagctgtt gagcgggaag
      421 atgaccagat caccgggctt gaattgcgga tgttcggcat cgacggcacg gacgccgcgg
      481 cgatccatgt agtattgaaa accggagtga ccggcaaacc acagccggcc ttgactgtaa
      541 gcctccgcca ccgctggaac actcagcagg cgatcgacca cgtcgcgaca gatgctggcc
      601 attcgatagt ccgcaacggc tacgcaaatg gcgagcgctg cggcgggggc gagcgcgatc
      661 gccagccagc gagcggacgg ggcgccaaaa cggtcgacgg cccgcgcgcc gaggatgcac
      721 gccgcgggaa ccagcggcag gatcgtgcgc gcgttgatcg cccaattcag gaacgtggca
      781 aacaccgcga ccccaccgat ccagagcagc agaaacacat cgtcgcgcca accggtgcgg
      841 cggaggcgct gggacatgaa cgatccgcag aggcagaaga tcgcgacccc ggcggcggcg
      901 agcaggccgt attgcaggaa gaatacccac gccgggccga tcggttgcgc tccgctccaa
      961 gccggcggtg gcgcgacgca gcgcgacgcc acgacgccca gcaggatcac cagcggcggc
     1021 agcgcgaacc cgcgcaggcg cggcggcgcg aggagcaccg cgagcaccgc cacggcaaac
     1081 acccccccgc cgatgaacgc cagcgaatcc aggatgccca gcaatggggg aatggtgatg
     1141 ccgctttggg tcaccttcac atggcttgcg gcgccgagca gcaaaccatt tccgtaatgg
     1201 atccgcgtga accattcgta ccccgcccac gcggcaatcg ggatcagcag tgacgcgatt
     1261 tggatgaggc gcgtccggcg gttggccggc caaagcgcgg cgcccgcgat cagcagcggc
     1321 agcaggttga tgcccggata tttcgtcaac cccgcggcgg cggtggatag gcccgccagc
     1381 ggcaaccaca tcgccgagcg ccgcgttccc caaacccaaa acagcacgga ccacacccag
     1441 aagcacaaca gcgggacatc gcacatcagc gtcgtggccg agacgagaaa tgcgggacag
     1501 cacaacagca atagcgccgc cagcgccggt cgccgagtga actgcgttgc cagcagatag
     1561 gtgccgacga ccgtgaggag gccgaacggc agcgccgccg cgtgcagggc gatttcgctc
     1621 caaccgacga tcgccgacat gcccgccagg tcatacgaaa agagcggcgg gttctgcagg
     1681 acctcgctca tcggctgttc gcgccattcc cagttgatca agccaccgta gaaatcgagc
     1741 gggtgccgcg tgatctgccg ggccggccag atgaagacgg gatcgtcgtt gtgcacgggt
     1801 ttggcgagga acggcaggag catcgccagg caaagcgcac atagcgcgat gagatgtttt
     1861 gcgttcaccg gcgtggccgg cggcagcgct ccgtgagctg aagtcatcag gtggcttggg
     1921 ctcgcggagg acagggtcat gtcgagttgc caggcgttt
//
LOCUS       DATIMH010000123         1502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1763509, whole genome shotgun sequence.
ACCESSION   DATIMH010000123 DATIMH010000000
VERSION     DATIMH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763509"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1502)
                     /locus_tag="VLI90_02075"
     CDS             complement(<1..>1502)
                     /locus_tag="VLI90_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13019.1"
                     /translation="IENADIENADIENADIENADIENADIENGSVADYSVDLKNDGNT
                     ATAYQVKFAVTGDTSPYLFQLIGRRVYKTPTAVGCEVKEAGQNQIFFNVNLTAADLQP
                     GAPPNPLDPSPTNTTVLVQPGEHIKVTLRAWDKDVLSGPGAPPNDGIQPFCPMLSPSC
                     PAVTTLVTTTVVSLSSNTGSNTPPVETFPSTPTQVPFSLIVTNTNDSGPGSLRQAITN
                     ANSHSGFTDTISFNIRGGGVHTILPSSPLPALTEPVIIDATTQLGYAGSPVIEIDGAN
                     AGTADGLVLTGASTVRGLAINRFAGNGLRLTGNGNTVQSSYIGTDSTGVADLGNLANG
                     IQIVDGANNLIGGPQPAVKNIVSGNQGEGIRIDGALATGNIVQGNFVGTNAAGTGALG
                     NSASGIYIRNAPGNSVIGNVVSGNTGFAGIAICGNASSCGGGNTGTQTSNATGNIVQG
                     NLVGTNAVGTGAVGNAQRGISIDAAPNTLVGGPLASHRNVVAATTAGPGI"
BASE COUNT          237 a          449 c          496 g          320 t
ORIGIN      
        1 gattcccggt ccggccgttg tcgccgcgac gacgttccta tgcgacgcga gtggcccgcc
       61 gacaagcgta ttgggcgcgg catcaatgct tattccgcgc tgcgcgttgc caaccgcgcc
      121 ggtgccgacc gcgttggtgc cgaccaggtt gccctggaca atgttgccgg tggcgttgct
      181 ggtttgggta cccgtgtttc cgcctccgca gctgctggca ttcccgcaga tcgcgatccc
      241 ggcgaatcca gtgttgccag acacgacgtt tccaatcacc gagtttcctg gcgcgttccg
      301 gatgtaaatg ccgctggcgc tgtttccaag cgcgccggtt cctgctgcgt tggtgccgac
      361 gaagttgccc tggacgatgt tgccggttgc cagcgcgcca tcgatcctga tgccttcgcc
      421 ctgattgccg gacacgatgt tcttgaccgc gggctgcggg ccgccaatga gattgtttgc
      481 gccatcgacg atctgaatcc cgttcgccag gttgccgaga tccgcaacac cggtcgaatc
      541 ggtgccgatg tagctggatt gcaccgtgtt gccattgccg gtgagtctca gcccatttcc
      601 agcgaaccga ttaatcgcca ggccgcgaac cgtgctggcg cccgtgagca cgagcccatc
      661 ggcggtcccg gcattcgcgc cgtcgatttc aatcactggc gagccggcat agccaagctg
      721 ggtggtggcg tcgatgatga ccggctcggt gagcgccggc agcggcgacg aggggaggat
      781 tgtgtgaacg ccgccgccac ggatgttgaa cgagatcgta tcggtgaacc cggagtgcga
      841 gttggcgttg gtgattgcct ggcgcagcga tcccggaccg ctgtcgttgg tgttggtcac
      901 gatcagactg aacggaacct gcgtcggcgt cgacgggaac gtttccactg gcggcgtgtt
      961 cgagccagtg ttcgacgaca gagacaccac ggtggtggtg accagagtag tgaccgccgg
     1021 gcagctcgga gacaacatcg ggcagaacgg ctggatgccg tcgttcggcg gcgcgccggg
     1081 gccgctgagc acatccttgt cccacgcgcg gagcgtgacc ttgatgtgct cgcccggctg
     1141 cacgagcacg gtcgtgttgg tcggggacgg atcgagagga ttgggcggcg cacccggctg
     1201 gaggtccgct gccgtcaggt tgacgttgaa gaaaatctgg ttctgtccgg cttccttgac
     1261 ctcgcatccg accgcggtcg gcgtcttgta gacgcgtcgc ccaatcagct ggaagagata
     1321 aggcgacgta tcgccggtga ccgcgaactt cacctggtaa gccgtcgcgg tgttgccatc
     1381 gttcttgagg tcgaccgaat agtcagctac ggagccgttc tcgatatcgg cgttctcgat
     1441 atcggcgttc tcgatgtccg cgttttcaat gtctgcgttc tcgatatcgg cattctcgat
     1501 at
//
LOCUS       DATIMH010000124         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3784856, whole genome shotgun sequence.
ACCESSION   DATIMH010000124 DATIMH010000000
VERSION     DATIMH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3784856"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..481)
                     /locus_tag="VLI90_02080"
     CDS             complement(119..481)
                     /locus_tag="VLI90_02080"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HSV13020.1"
                     /translation="MHNIPALCRFFGIIIRMYMESGAPHHLPHFRAYYQEHVAVIGID
                     PVEVIAGEMPRRQQRLVEAWAELHHDEQMENWRRLQPGEAASPIPPLAKWWWYYDSPH
                     LPRRFIQDRSAIYAKRAI"
     gene            complement(486..1550)
                     /gene="mtnA"
                     /locus_tag="VLI90_02085"
     CDS             complement(486..1550)
                     /gene="mtnA"
                     /locus_tag="VLI90_02085"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HSV13021.1"
                     /translation="MLETIGWTGGAMRLIDQTKLPTETVYVQIHDERQTWDAIKRLVV
                     RGAPAIGVAAAFGVYLGVRAGLDDRASFDRRLNQVCDYLITARPTAVNLRWAVDRVRR
                     VAQSGASVNDAKQRLLDECLAMLDEDRRACRAIGEHGMALLAEGATARQPIGILTHCN
                     AGALATVQYGTALSPIYIGAERGLRFHVYSDETRPLLQGSRITACELQSAGIPVTVIC
                     DNMAAVVMQQGKVDAVIVGTDRVAANGDVANKIGTLGVEILARHFNIPFYVAAPISSI
                     DVSLASGAEIPIEQRDASEVTNGLGRQTAPTGVDVYNPAFDVTPADLVSAIITERGVL
                     RPPFSESIRNAIGSESVESA"
BASE COUNT          290 a          526 c          563 g          335 t
ORIGIN      
        1 gagcgtgtgg acgtcggggt cgatcctgac cccattgaag atggtgatgt ccagtagcgg
       61 cgcaagcagc tgcccgccga gcacgggtcg gaagtcgatc aactgcgatg acccgtcatc
      121 aaatcgcacg cttagcgtat atggcgcttc gatcttgaat gaaacgacgc ggtagatggg
      181 gtgaatcata ataccaccac cactttgcga gtggcgggat cgggcttgca gcctcgcccg
      241 gctgcaggcg gcgccagttt tccatttgct cgtcgtggtg caactccgcc cacgcctcga
      301 cgagtcgctg ttgccgtcgc ggcatttcgc cggcgatcac ctcgacagga tcgatcccta
      361 tcaccgccac gtgctcctga taatacgcac gaaaatgcgg cagatggtgc ggcgcgccgg
      421 attccatgta catgcggatg atgatgccga agaatcggca cagtgccggg atattgtgga
      481 ttgtatcacg cggattcgac gctttcgctt ccgatggcgt ttcgtatcga ttcgctgaat
      541 ggcgggcgca gcacgccgcg ttcggtgatg attgccgaga ctaaatcggc gggcgtcacg
      601 tcgaatgcgg ggttgtagac gtccacgccc gttggcgcgg tttgccggcc aagcccgttt
      661 gtcacttcgc tcgcgtcgcg ctgttcgatg gggatctcgg cgccgctggc aagcgagacg
      721 tcgatgctgc tgatcggggc ggcgacgtag aacgggatgt tgaaatgccg cgccaggatc
      781 tccacgccga gcgtgccgat cttgttggcg acgtcaccgt tggcggcgac gcggtcggtg
      841 ccgacgatga ccgcgtccac cttcccctgc tgcatgacga cggcggccat gttgtcgcaa
      901 atgaccgtga ccggaatgcc ggccgactgc agctcgcacg cggtgatccg gctgccttgc
      961 agcagcgggc gggtttcgtc ggagtagacg tggaaacgca gcccgcgctc ggcgccgatg
     1021 tagatcggcg acaacgcggt gccgtactgc accgtcgcca gtgccccggc gttgcaatgc
     1081 gtgaggattc cgatcggctg ccgcgctgtt gcgccttcgg ccaacaaggc catgccatgc
     1141 tcgccgatcg cccggcacgc ccggcggtct tcgtccagca tcgcgaggca ctcgtcgagc
     1201 aatcgctgct tggcatcgtt gaccgatgcg ccagattgag ccacccgccg cacgcggtcc
     1261 accgcccagc gcaggttcac cgcggtgggg cgggcggtga tgagatagtc gcagacttgg
     1321 ttcaatcggc gatcgaacga cgcgcggtcg tccagtccgg cgcgcacacc aaggtaaacg
     1381 ccgaacgcgg cggcgacgcc gatggcgggc gcgccgcgca cgaccagccg cttaatggcg
     1441 tcccacgttt ggcgctcatc gtgaatttga acgtacaccg tctcggtcgg cagcttcgtc
     1501 tggtcgatca gccgcatcgc gccgccggtc cacccaatcg tctcgagcat cgtcatgaag
     1561 ttaccgcggc gaaatcgaca tgtatatcga gcgcgtgtct tggcgaggca gccgggtgta
     1621 actgaacttt gtggttcgac gcttctctgg tcccctcgcc cggtactgct gaccgcaggt
     1681 ctccgggaga gggttagggt gagggtgatt tggt
//
LOCUS       DATIMH010000125         1539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3785679, whole genome shotgun sequence.
ACCESSION   DATIMH010000125 DATIMH010000000
VERSION     DATIMH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3785679"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..524
                     /gene="rpsH"
                     /locus_tag="VLI90_02090"
     CDS             123..524
                     /gene="rpsH"
                     /locus_tag="VLI90_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HSV13022.1"
                     /translation="MSMTDPIADMLTRIRNACGSKHRRVDMPVSKMKTEIARILKEND
                     YIQDYATVEGEDGRPLLRVRLKYAAGGQPVIRALQRVSTPGLRKYVGVTEVPRVRNGL
                     GIAILSTSKGLMSDREARQARTGGELLALIW"
     gene            537..1079
                     /gene="rplF"
                     /locus_tag="VLI90_02095"
     CDS             537..1079
                     /gene="rplF"
                     /locus_tag="VLI90_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSV13023.1"
                     /translation="MSRIGKAPIAVPNGVTITINDGNNITVKGPKGELTRTVPAAMGI
                     TQEGGQVTVTRPSEESNHKALHGLTRTLIANMVEGVTKGFTKNLELVGVGYKAEKRPY
                     GLQLSLGYSHAIEYKAPKGITLTAPVPTQILVEGANKEVVGQVAAEIRSLRPPEPYKG
                     KGVKYAGEQIRRKAGKAGGK"
     gene            1082..1441
                     /gene="rplR"
                     /locus_tag="VLI90_02100"
     CDS             1082..1441
                     /gene="rplR"
                     /locus_tag="VLI90_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HSV13024.1"
                     /translation="MGRIGIPKNRAEKRVRRHLRVRKTVQGTAERPRLVVFRSLKHIT
                     AQLVDDGAARTIATVTSVKVGEGKKSEKSLEVGKQIAAIAKDKGISKVVFDRGGYQYH
                     GRVKAVADGAREGGLEF"
BASE COUNT          304 a          480 c          541 g          214 t
ORIGIN      
        1 cttcgctgcg ccgcggctcc cgaggcccgc cgacaagccg ttcaccgaaa aaccgttcac
       61 ggcagttcac actcaatccg ccacgtcctc cgggatacga gcggaccaag gatttctgtt
      121 caatgagcat gaccgatccg atcgccgaca tgctgacgcg gatccgcaac gcctgcggat
      181 cgaagcaccg ccgcgtcgac atgccggtgt cgaagatgaa gaccgagatc gcgcggatcc
      241 tgaaggagaa cgactacatt caggactacg cgaccgtcga gggtgaggat ggcaggccgc
      301 tgctccgcgt gcgtctcaag tacgccgccg gcggacagcc cgtcattcgc gcactccagc
      361 gcgtgtccac tccgggcctg cgcaagtacg tcggcgtcac cgaggtcccg cgcgtgcgca
      421 acgggctcgg catcgcgatc ctgtccacgt cgaaggggct gatgtccgat cgggaagcgc
      481 gtcaggcgcg caccggcggc gagctcctcg ccctcatctg gtaaggggcg accaacatgt
      541 cacgtatcgg taaagctccg atcgccgttc cgaacggcgt cacgatcacg atcaacgacg
      601 gcaacaacat cacggtgaag ggcccgaagg gcgagctcac ccggacggtg ccggcggcca
      661 tggggatcac ccaggagggt ggccaggtca ccgtgacgcg cccgtccgaa gagtcgaacc
      721 acaaggcgct gcacggtctg acgcgcaccc tgatcgccaa catggtggaa ggggtgacga
      781 agggcttcac gaagaacctc gagctggtcg gcgtcggcta caaggcggag aagcgcccgt
      841 acgggctgca gctctcgctg ggctactcgc acgcgatcga atacaaggcg ccgaagggga
      901 tcacgttgac cgctccggtg ccgacgcaga tcctcgtcga gggcgcgaac aaggaagtgg
      961 ttggccaggt ggcggcggag atccgcagcc tgcgtccgcc cgagccatac aaggggaagg
     1021 gcgtcaagta cgcgggcgag cagattcgcc gtaaggccgg caaggcggga ggcaagtaat
     1081 catgggccgc atcggaattc cgaagaatcg cgcggagaag cgcgttcgcc gtcacctgcg
     1141 cgttcgcaag accgtacagg ggaccgccga gcgtccgcgc ctggtggtgt ttcgctcgct
     1201 gaagcacatc accgcccagc tggtggacga tggcgcggcg cgcacgatcg cgacggtcac
     1261 gtcggtgaag gtgggcgagg ggaagaagtc ggagaagtcg ctcgaagtcg gcaagcagat
     1321 cgccgcgatc gccaaggaca aggggatctc aaaggtcgtc ttcgaccgcg gcgggtatca
     1381 gtatcacggc cgcgtgaagg cggtggccga cggggcccgc gaaggcgggc tggagttcta
     1441 acatggaatc gaacagcaat gccggcggtg cgccggccgg tggacagggc ggtggcagct
     1501 cccagggcgg cggtgcgcgc tccggcggct tcggcggcg
//
LOCUS       DATIMH010000126         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2052848, whole genome shotgun sequence.
ACCESSION   DATIMH010000126 DATIMH010000000
VERSION     DATIMH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052848"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VLI90_02105"
     CDS             <1..971
                     /locus_tag="VLI90_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13025.1"
                     /translation="QLAQRWHYLLLHEKSFGMTRQAFDEVARTADVFLNVSGACFIPE
                     NLNPRAIKVFLDTDPGYNQFVLSERYAWSQNADRWAQTVAAHDRHLTYAENIYGDDCL
                     IPRLNFDWRPTRCVVTLPSWASIRNSPPLADAPFTTVMTLGYFKGPVVYKGKTYDAKV
                     PEFEKFRALPRRTNTPLLMAIGGDMVKLDPIRADGWNLVDAKDVTLTTERYMQFIAES
                     AGEWSVAKNVYVDTNSGWFSCRTACYLAAGRPAVVQETAWSRFVPSGRGVIAFKTMEQ
                     CTAALEEVTAHPQKHREAAYEIAREYLAPDRVMPPMLDEIFAAPKW"
     gene            complement(1022..2146)
                     /locus_tag="VLI90_02110"
     CDS             complement(1022..2146)
                     /locus_tag="VLI90_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HSV13026.1"
                     /translation="MRDPRLAKLAQVLVNYSVGVKRGQLVRISGPPVATPLVVELFRE
                     TVKAGGNPMVRMSPEELTEILIKNAGDDQLKYLNPVTQFEYERIDCSIGIWAEENTKA
                     LSNVDSKKLGLTQAARKPLMDIFMKRAASGALKWSGTQFPCQAAAQDAEMSLAEYEDF
                     VFNAGLLNQPDPVAAWKKMSERQQRLVDLLQGKQDYHVVANNGTDVRMSLAGRTWINC
                     DGHENFPDGEVFTGPVLDSVNGTIHFSFPAVHIGREVQDVVLKFKDGKVVDAHAAKGE
                     DFLISMLDMDGGSRMLGECAIGTNYQIQQYTRNTLFDEKIGGTVHFALGAGYPETGNS
                     NQSGLHWDMVVDLRAGGFIEINGEKINVDGKFMREGFPGL"
     gene            2237..2656
                     /locus_tag="VLI90_02115"
     CDS             2237..2656
                     /locus_tag="VLI90_02115"
                     /inference="COORDINATES: protein motif:HMM:NF018889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1425 domain-containing protein"
                     /protein_id="HSV13027.1"
                     /translation="MAYDADLRLRSAIVASIAIVALSCTRSPPANERLQHSASPDGRI
                     AFADAGLRDQLGLGPLALARDDAQLLHVTLPVRSTTGLAQDINWRITWLDANGIPLGP
                     PTPWSTAAVTTDDFRQILATSWTPRATDARLELRYAK"
     gene            2780..2861
                     /locus_tag="VLI90_02120"
     tRNA            2780..2861
                     /locus_tag="VLI90_02120"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2814..2816,aa:Tyr,seq:gta)
     gene            2888..>3094
                     /locus_tag="VLI90_02125"
     CDS             2888..>3094
                     /locus_tag="VLI90_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13028.1"
                     /translation="MNFDYGPQKGPFLFVVETACIVMRPCASCSRPLASAANLFDRRV
                     YKKFTPKLDASGHLGSRCFWLRKEH"
BASE COUNT          578 a         1038 c          883 g          595 t
ORIGIN      
        1 cgcagttggc acagcggtgg cattacctgc tgctgcacga aaagagtttc ggcatgacgc
       61 ggcaagcttt cgacgaagtc gcgcggacgg ccgatgtttt tctcaacgtc agcggcgctt
      121 gcttcatccc cgaaaacctg aacccgcgcg cgatcaaagt cttcctcgac accgaccccg
      181 gctacaacca gttcgtgctg tccgagcgat atgcgtggtc gcaaaatgcg gatcgatggg
      241 cgcagaccgt cgcggcacac gatcggcacc tgacgtacgc cgagaacatt tacggcgacg
      301 actgcctcat cccgcggctc aacttcgatt ggcggccgac gcgctgcgtc gtcacgctgc
      361 cgtcgtgggc ttcgatccgc aattcaccgc cgctggccga tgcgcctttt accaccgtca
      421 tgacgctcgg ctatttcaaa ggccccgtgg tctacaaggg caagacgtac gatgcgaagg
      481 tgccggaatt cgaaaagttc cgcgcgctgc cgcggcggac caacacgccg ctgctgatgg
      541 cgatcggcgg cgacatggtg aagctcgatc caatccgcgc cgacggctgg aacctggttg
      601 acgccaagga cgtcacgctg acgaccgagc gatacatgca gttcatcgcc gagtccgccg
      661 gtgaatggtc cgtcgcgaag aacgtgtacg tagacaccaa cagcggctgg ttcagctgcc
      721 gcaccgcgtg ctatttagcc gcgggtcgac cggcggtggt gcaggagacc gcctggagcc
      781 gcttcgtgcc gagcggtcgc ggcgtcattg cattcaagac gatggaacaa tgcaccgcgg
      841 cgctagaaga agtgacggcc cacccgcaga aacaccgcga agcggcgtac gagatcgcgc
      901 gcgaatatct cgcgcccgat cgagtcatgc cgccaatgct ggacgagatc tttgctgcgc
      961 caaaatggta gccgcgacgc ccgcgtcacc tctgcccgcg aaggcgacgc aggcgtcgcg
     1021 gctacaagcc cggaaatcct tcgcgcatga actttccgtc cacattgatc ttctcgccgt
     1081 tgatttcgat gaaccccccg gcccgcaggt ccaccaccat gtcccagtgc aggccgctct
     1141 ggttcgagtt gccggtctcg ggatatccag cgcccagcgc gaaatgcacg gtcccgccga
     1201 tcttttcgtc gaacagcgtg ttccgcgtgt actgctgaat ctgatagttc gtgccgatcg
     1261 cgcactcgcc gagcatccgg ctgccgccgt ccatgtctaa catgctgatc aaaaaatcct
     1321 cgcccttggc cgcgtgggca tcgacgacct ttccgtcttt gaatttcagc accacgtcct
     1381 gcacttcccg cccgatgtga acggcgggga agctgaagtg gatggtgccg ttgacgctgt
     1441 ccagcaccgg gccggtgaag acttcaccgt ctgggaagtt ctcgtggccg tcgcagttga
     1501 tccacgttcg cccggccagt gacatgcgca catccgtgcc gttgtttgcc acaacgtgat
     1561 agtcctgctt cccctgcagc agatcgacca accgctgctg ccgctcgctc atctttttcc
     1621 acgccgccac cggatcgggt tgattcaaca gccctgcgtt gaagacgaaa tcttcgtact
     1681 cggccaagct catctccgca tcctgcgccg ccgcctggca ggggaactgc gtgccgctcc
     1741 acttcagcgc gccgctggcg gcgcgcttca taaaaatatc catcagcggc ttgcgggccg
     1801 cctgcgtcag gcccagcttt ttcgaatcga cgttcgacag cgccttggtg ttctcttccg
     1861 cccagatgcc gatcgagcaa tcgatccgct cgtattcgaa ttgtgtgacc ggattcaaat
     1921 atttgagttg atcgtcaccc gcgttcttga tcaaaatctc ggtcagctcc tccggcgaca
     1981 tccgcaccat cggattccca cccgccttca ccgtctcgcg aaagagttcc accaccagcg
     2041 gcgtcgccac cggcggtccg ctgatgcgga ccagttgccc gcgcttcacg ccgacgctgt
     2101 aattcaccaa cacctgggcc agcttcgcca atcgaggatc gcgcatagcg ggactgtagt
     2161 ccccgcgcga gaacgaggca acgcggcgcg gaactgcgaa gcagggcagc gaaggtggcg
     2221 cgcgaagagg tggcgtttgg cgtatgatgc ggacttgcgc ctccgcagcg ccatcgtcgc
     2281 cagcatcgcc atcgtcgcct tgagctgcac ccgatcgccg ccggcgaatg agcgtctgca
     2341 gcattcggca tcgcccgacg gccgcattgc cttcgccgat gccggccttc gggatcaact
     2401 cgggctcggc ccgctcgcgt tggcccgtga cgatgcccag ttgctccacg tcacgctgcc
     2461 cgtgcgatcg acgacgggct tggcacaaga catcaactgg cgcatcacct ggctcgacgc
     2521 caacggcatc ccgctcggcc cgccaacccc gtggtccacc gccgccgtga cgacggatga
     2581 tttccgccag atcctggcca ccagctggac cccgcgcgcc accgacgcac gactggaact
     2641 acgctatgcc aagtagccgc ctcgaccgga gtcgaccacg aagcatggcc ccatcgcggc
     2701 agctattgaa accccggcca gttccgccgt atattcatcg acccgccgcc gagccccggc
     2761 cgcggatcgg gtcgtacacg ggcgtgtacc taagtggcca aaaggggcgg actgtaaatc
     2821 cgctggctct gccttcgcag gttcgaatcc tgccgcgccc attgaaataa cgtcccggca
     2881 ggcgtttatg aatttcgact acggccctca aaaagggccg tttttatttg tagttgaaac
     2941 ggcttgcata gtcatgcgac cctgcgcctc ctgttctcga ccgctggcgt cagcggcaaa
     3001 cctttttgac aggagagttt ataaaaagtt cactccaaaa ctcgatgcca gtggtcatct
     3061 tggatcgcgg tgtttctggc tccgcaaaga gcac
//
LOCUS       DATIMH010000127         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1476776, whole genome shotgun sequence.
ACCESSION   DATIMH010000127 DATIMH010000000
VERSION     DATIMH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476776"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1565
                     /locus_tag="VLI90_02130"
     CDS             <1..>1565
                     /locus_tag="VLI90_02130"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HSV13029.1"
                     /translation="PRTDEVDHPGYYAATLTRYSTRAELTASRRVALHRYTFPASDES
                     HLTLNLDHCLNKGTGWEDQKFLGGEVHIVSDHEAEGVGRYTGGWNRGGEYRVYFYIVL
                     DTPSRTQQIWTGKTLAGKRDATIASDEPFGLSFDFHTKARQAIEAKVAISFVSGAQAR
                     ESSQQEMPGWNFIAARQAATAAWNKALSPMQVRGTTPEQRREFYTALYHVMLMPSDRT
                     GENPVWQSNEPYYDDFQAIWDTFRSSGPLLTLIAPDRQRDMLRSLIDTYRHTGYMPDA
                     RVGNDNGRTQGGSNANILVADAYVKGMTGIDYPTAFAAMLKDAEVPPDDTQKEGRGGL
                     HDYNTRGYVTLADQRSGSRTVEYSYDDYGIAEVACGLNRDKEAKIFAARSRNWQNLWD
                     KDLANDGFTGFLRPRNADGTWAPPNTLVRGTWPDFFYEGDLWTYSLYAPHDVRKLIEL
                     SGGKENFVRRLDRLFLRQHFDVTNEPGFLLPVLYNWAGRPDHTADVVRAYLDKYFTAR
                     RSGIPGNDDSGAMS"
BASE COUNT          330 a          529 c          436 g          270 t
ORIGIN      
        1 ccacgcacgg acgaggttga ccatcccggc tactacgcag ccaccctcac ccgctactca
       61 acgcgagccg aactcaccgc gtcccgccga gtcgcactgc accgctatac atttcccgcc
      121 tcggatgaat cccacctgac actcaacctc gatcattgcc tcaataaagg aaccggctgg
      181 gaggatcaga agttcctggg cggcgaggtg cacatcgtct ccgaccacga agccgaaggc
      241 gtaggacgct acaccggcgg atggaatcgt ggtggcgagt atcgcgtcta tttctacatc
      301 gtcctcgata ccccttcccg aacccagcag atctggaccg gcaagactct cgcgggcaag
      361 cgcgatgcga caatcgcgtc cgatgagccc ttcggcctct ccttcgactt ccacaccaag
      421 gcgcgccagg cgatcgaggc gaaggtcgcg atctccttcg tcagcggagc gcaggcccgc
      481 gaatcttccc agcaggagat gcccggctgg aactttatcg cagcacgtca agcggctacc
      541 gccgcatgga ataaagcgct ctcgccgatg caggtgcgcg gcacgacacc ggaacagcgt
      601 cgcgagtttt acactgcgct ctatcacgtc atgctgatgc cgtccgaccg caccggcgaa
      661 aatccagtgt ggcaatcgaa cgaaccctac tacgacgatt tccaggcaat ctgggatacc
      721 ttccgcagct ccggtccgct gcttaccctg attgcgccag atcgccagcg cgacatgctg
      781 cgctcgctca tcgacaccta ccgccacacc ggatacatgc cggatgcacg cgttggcaat
      841 gacaacgggc gtacgcaggg cggcagcaac gcgaacatcc tcgtcgccga cgcttacgta
      901 aaaggcatga ccggcatcga ctatcccact gcgtttgccg cgatgctgaa ggacgccgag
      961 gtgccgccgg acgacaccca gaaggaaggt cgtggcggtc tgcatgacta caacacccgc
     1021 ggctacgtca ccctcgccga ccagcgctcc ggctcgcgca ccgtcgaata cagctacgat
     1081 gattacggca ttgcagaggt cgcatgcgga ctcaatcgcg acaaggaagc gaaaatcttc
     1141 gccgcacgat cccgcaactg gcagaatctc tgggacaagg atctggcgaa cgatggcttt
     1201 acgggcttcc tgcggccgcg caacgccgat ggaacctggg cgccgcccaa tacactggtg
     1261 cgcggcacat ggccagactt cttctacgag ggagacctct ggacctactc gctgtacgcg
     1321 ccgcacgatg tgcgaaagct catcgaactc tccggcggca aggagaactt cgtgcgcaga
     1381 ctcgatcgcc tgtttctccg ccagcatttc gacgtgacga acgaaccggg attccttctg
     1441 cccgtgctgt acaactgggc cggccggccg gatcacacgg ccgatgtggt ccgtgcctat
     1501 ctggataagt acttcaccgc gcgtcgcagt ggaattcccg gcaacgacga ttccggcgcg
     1561 atgtc
//
LOCUS       DATIMH010000128         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_33128, whole genome shotgun sequence.
ACCESSION   DATIMH010000128 DATIMH010000000
VERSION     DATIMH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33128"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..786
                     /locus_tag="VLI90_02135"
     CDS             151..786
                     /locus_tag="VLI90_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HSV13030.1"
                     /translation="MPPIPERRLVEFVDACHRVAAYGLLKCSSGNLSCRLDEERVMIT
                     ATRSWLADLTLDQVSLLRLSDGQQLNDAKPSVETRFHLGILRQRPDVNVVLHFQSPAA
                     TTITCGDPARVNFFVIPEIPYYIGPIAVLPYIDPGSADLAAAVVPAMRDHNLAILTHH
                     GQVTVGRDYNEALQRAAFFELACDMICRGGDGVHALTATAVEQLLRAGKPI"
     gene            898..2718
                     /locus_tag="VLI90_02140"
     CDS             898..2718
                     /locus_tag="VLI90_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV13031.1"
                     /translation="MRLYLRAISYFRPDWPLVLLWLALIGISTGLGLLGAWPMAILID
                     SVLSTSGAHDGIHRLFLAPLPTSLVGRVIGLAVVGLVLKLMQEGLSIGQTIVSNHINY
                     SGLVRVRCDLYRKLQALHLAYHRSQPQGDAIYRLSTDTFGCQTILGVLVNTIVAAATL
                     VTMTIILGYRNLPLTLIAFSIAPILAGANVFFGRRFRERTLECKDIDTRFTTVVQRSM
                     ACIGLVQSFGREAEEFAHFHGTVREAVRAWWRLNWQQMAYNLIVGATFGIGGAVVFGY
                     GGYLVYRDQIVRHTQSGMTVGDLVVFTSYLGMLWGPLCSMTGFFYNIQGGVAGAQRIF
                     EVLDRDPAIVNKPNARPLPRQPRRLQLDHVTFAYDNGKPVLRDVSVTIEPGEMVAFVG
                     ASGVGKSTLLNLVPRFYDPTDGAIRFDGIDARDVKIADLRRHVALVLQDSVILPTTIA
                     ENIAYGRQHATLAQVRRAAEMAGAAEFIEQLPEGYNTQINEGGQNLSGGQRQRISIAR
                     ALLTEAPFVILDEPTSALDPLHERHITQALRRLKRQRTVILVSHRLSTVIDCDRIFVM
                     HGGAVVEHGAHPELLAKGGLYATMAQHQLSDGDPSRKRAA"
BASE COUNT          485 a          937 c          838 g          507 t
ORIGIN      
        1 cgatttctca attgcagcat cgcgcctgat cgcaaggtcc gcgcgcatgt cattctccaa
       61 cgcaggcgta ctcgccggag gatgacgttg aagttcacgc cgggctgcat ggggttgttc
      121 tgtgctcgac cttctccgat gatttgacac atgcccccca ttccggagcg acgactggtc
      181 gagtttgttg acgcctgcca tcgcgttgcc gcgtatgggc tgctgaagtg cagcagcggg
      241 aacctctcgt gccgccttga tgaggagcgc gtgatgatca ccgcgacgcg aagctggctg
      301 gcggacctga ccctggatca ggtctcgctg ttgcgattga gcgatgggca gcagctgaac
      361 gacgccaagc cgtcggtgga gacgcggttt catttgggaa tcctgcggca gcggccggac
      421 gtgaacgtcg tgctgcactt ccaatccccc gcggcgacga cgatcacctg cggtgatccg
      481 gctcgcgtca acttcttcgt cattccggaa attccctact acatcgggcc gatcgcggtg
      541 ctgccataca tcgaccccgg ctcggccgac ctggcggcgg cggtggtgcc ggcgatgcgc
      601 gatcacaatc ttgccatcct cacccaccac ggtcaggtga ccgtcggccg cgattacaac
      661 gaagcgctgc agcgagcggc gtttttcgag ctggcgtgcg acatgatctg ccgcggcggc
      721 gacggcgtgc atgccctcac cgccacggcg gtggagcagt tgctgcgagc ggggaagccg
      781 atttgagtgc aagccaaagg acgccccatt ccatagcccg tgctatgaaa agcggagcac
      841 caagattttt cgccgcactt ttcgaagcaa agtgctcgag ctaccgataa tcctgatgtg
      901 cgactttacc tccgtgcgat ttcgtatttc cgccccgatt ggccgctcgt gctgctttgg
      961 ctcgcgctga tcggcatctc cacgggcctg ggcctcctgg gggcgtggcc gatggcgatc
     1021 ctcatcgact cggtcctctc cacttcgggc gcgcacgacg ggattcaccg cctgttcctg
     1081 gcgcccctgc ccacgagcct cgtcggccga gtgatcgggc ttgcggtcgt cgggctggtc
     1141 ctgaagctga tgcaggaagg cctgtcgatc gggcagacga tcgtcagcaa tcacatcaac
     1201 tacagcgggc tcgttcgcgt ccggtgcgat ctgtaccgca agctccaggc gctgcacctg
     1261 gcgtatcacc gcagccagcc gcagggcgat gcgatctatc gcctcagcac cgacacgttt
     1321 ggttgccaga cgatcctcgg cgtgctcgtc aacacgatcg tcgccgccgc caccttggtg
     1381 acgatgacga tcatcctcgg ctaccgcaac ctgccgctga cgctgatcgc gttctcaatc
     1441 gcgccgatcc tggctggagc caatgtcttc ttcgggcggc gctttcgcga gcgcaccttg
     1501 gaatgcaagg acatcgacac gcgcttcacc accgtggtgc agcgatcgat ggcgtgcatc
     1561 ggcctcgtgc aatccttcgg tcgcgaagcc gaggaattcg cccacttcca cggcaccgtg
     1621 cgcgaagccg tccgcgcgtg gtggcgattg aactggcagc agatggccta caacctgatc
     1681 gtcggcgcga ccttcggcat cggtggcgcg gtcgtcttcg gctatggtgg ctacctcgtc
     1741 tatcgagacc agatcgttcg ccacacccag agcggaatga ccgtcggcga tctcgtcgtc
     1801 ttcacctcgt acctcggcat gctctggggg ccgctctgca gcatgactgg tttcttctac
     1861 aacatccaag gcggtgtggc cggcgcgcag cgcatattcg aagtcctgga ccgcgacccc
     1921 gccatcgtca acaaacccaa cgcccgcccc ctcccgcggc aaccgcggcg gttgcagctg
     1981 gatcacgtca ccttcgccta cgacaacgga aaacccgtgc tgcgcgatgt gtcggtcacc
     2041 atcgaaccgg gtgaaatggt cgccttcgtc ggcgccagcg gcgtcggcaa aagcacgctg
     2101 ctcaacctcg tgccgcgctt ctacgatcca accgatggcg cgattcgctt cgacggcatc
     2161 gacgcgcgcg acgtgaagat cgccgacctt cgccggcacg tcgcgctggt gctgcaggac
     2221 agcgtcatcc tgccgacgac gatcgcggag aacatcgcct atggccggca gcacgcgacg
     2281 ctggcgcagg tgcgccgcgc cgccgagatg gccggagcgg ctgagttcat cgagcaattg
     2341 cccgagggct acaacacgca gatcaacgaa ggcggccaga atctatccgg cgggcaacgt
     2401 cagcgcatca gcatcgcccg cgcactgctg accgaagcgc cgttcgtcat cctggatgaa
     2461 cccaccagcg cgctcgatcc cctgcacgaa cgccacatca cccaagcgct ccgccgcttg
     2521 aagcggcagc gcacggtcat cctggtcagc caccgcctga gcaccgtgat cgactgcgac
     2581 cgcatcttcg tcatgcacgg cggcgcggtc gtcgaacacg gcgcgcaccc ggaactgctc
     2641 gccaagggcg ggctatatgc gacgatggcc cagcaccagc tgagcgatgg cgacccctca
     2701 cgcaagcgcg ccgcgtaaaa cacgcgacac gcaatgaagc accccacgcc gggttcggag
     2761 gtactcc
//
LOCUS       DATIMH010000129         1589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3786577, whole genome shotgun sequence.
ACCESSION   DATIMH010000129 DATIMH010000000
VERSION     DATIMH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3786577"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..177)
                     /locus_tag="VLI90_02145"
     tRNA            complement(104..177)
                     /locus_tag="VLI90_02145"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(141..143),aa:Met,seq:cat)
     gene            341..1306
                     /locus_tag="VLI90_02150"
     CDS             341..1306
                     /locus_tag="VLI90_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HSV13032.1"
                     /translation="MKAIAREIVRRCSHGERVALCTVVGTKGSTPQARGAKMLVLAGG
                     QTLGTLGGGCVEAEVRSQAIKLLSTGTSKALRFSLDHDYGWDDGLICGGNMEIFIDVL
                     RDRDVDRFAEIADALEQERSATFEFRYAIDEREQRYTEDLGPPPVLVIAGAGHVGQAL
                     AALAAELEYRVDVIDDRVEYLTEARFPRARARIIGEIESALREYPINADTYVVIVTRG
                     HRNDGRALAAVVSSPAKYVGLIGSKRKIKAIMDDLAAQGVDRSSLLRVHAPIGFDIGA
                     VTVPEIALSIAAELVAVRRGCADRAVEPMKIQPAQLEQWLDRERR"
BASE COUNT          292 a          465 c          553 g          279 t
ORIGIN      
        1 gagtcccctt tcggtcgaca gggtacaaac tagggtacaa acgaccgaat cagaccaata
       61 aaaaagggac tcgcgttgtc gcaagtccct tctctgactg aagtagcggg gggaggattc
      121 gaacctccga cctccgggtt atgagcccga cgagctacca agctgctcta ccccgcgatc
      181 ttggtttgcc cattctaagc cacacgatga aagtgtcaag cgtggcggag aattatacga
      241 cctcgccgcg cggcggttcg cattcgagcg cgataagatt gacgtcgcgg gacaaaggag
      301 agccaaggcc gtcctcggcc gggttttgcc aacctcgtca atgaaagcca tcgcccgaga
      361 aatcgtccgc cgctgctcgc atggcgagcg ggtggcgctg tgcaccgtgg tcggcaccaa
      421 gggcagcacg ccgcaggcgc gcggggcgaa gatgcttgtg ctcgccggcg ggcaaacgct
      481 gggcacgctg ggcggcgggt gcgtggaggc ggaggtgcgg agccaggcga tcaagttgct
      541 ttccaccggc acgtctaaag cgctgcgctt cagcctcgac cacgactacg gctgggacga
      601 cggccttatc tgcggcggga acatggagat cttcatcgac gtgcttcgcg atcgtgatgt
      661 cgatcgcttt gccgaaatcg ccgacgcgct ggagcaggaa cgatcggcca cgttcgagtt
      721 tcgttacgcg atcgacgagc gggagcagcg atatacagag gacctgggcc ccccgccggt
      781 gctggtgatc gccggcgccg ggcatgtggg ccaagcgctg gcggcgctgg cggccgaact
      841 ggaatatcgt gtcgacgtga ttgacgatcg cgtcgagtac ctgacggaag cgcgcttccc
      901 gcgggcgcgg gcgcgcatca tcggtgagat cgaatcggcg ctgcgcgagt acccgatcaa
      961 cgccgacacg tacgtcgtca tcgtcacccg cgggcaccgc aacgacgggc gggcgctggc
     1021 cgcggtcgtc tcgtcgccgg cgaaatatgt cgggctgatc ggatcgaagc ggaagatcaa
     1081 ggcgatcatg gacgacttgg cggcgcaggg cgtggatcgg tcttcgctgt tgcgcgtgca
     1141 tgcgccgatc gggttcgaca ttggcgcggt gacggtgccg gagatcgcgc tgagcattgc
     1201 ggcggagctg gtggcggtgc gccgcggctg cgccgatcgc gcggtggagc cgatgaagat
     1261 tcagccggcg cagttggagc aatggctgga tcgggaacgc cgctgatcgt atcatgcccc
     1321 ctcttcgcga tgcgtctcgt ctagcggctg cattttcgcg gccgctaaac gagtgacggc
     1381 gaaacgatcg aacagcgcgg gccatcgcga ttttgtgtcg gcaaaggagt gccgggcgtg
     1441 ccgggtgaaa ctgagcttca caagacgttg aagaaggaag cctgccgctg gctgttccgc
     1501 atggggtacc gctgcatcgc ggcggaggtg cgcctcaaac cgctcggcat catcgatgcc
     1561 gtcggcaccg ggattttccg gccctatca
//
LOCUS       DATIMH010000130         1727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2053637, whole genome shotgun sequence.
ACCESSION   DATIMH010000130 DATIMH010000000
VERSION     DATIMH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053637"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VLI90_02155"
     CDS             <1..835
                     /locus_tag="VLI90_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSV13033.1"
                     /translation="TKRWKTVSMNKSFHGRSLAMIAATGNPAVRVGFEPEVPGFTHVD
                     LGDMEGLAAAVDSETAAVIIEPIQGEGGIHITPTDYIVELRRLCDERKVALIFDEVWT
                     GCGRTGKWFGHQYFTTASGKCVEPDIMTLGKAVGGGLPVGVMWAKPEIAKLFVPGKHG
                     STLGGNPICMAVSRTIFDVIERERLTDNAATLGEYGMARLKSEKRIAPKITAVRGRGL
                     MIGVELKETPEKLVEKGLAKGVLLNLTSKNVIRLAPPINITREDWDRGLDRVIETIAS
                     L"
     gene            928..>1727
                     /locus_tag="VLI90_02160"
     CDS             928..>1727
                     /locus_tag="VLI90_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13034.1"
                     /translation="MKRCLLSIFTLHALVGCQSHRAPTAAPTTRPALPTTMQISLSAH
                     PLQPADPRDPPPELSLLIHLDIYQLDVPVGAVSRNEALWKRVNEQTVGAGEGTTDLLF
                     KNGLRCGVAPRSEWSFFRDLLGGQPGRMRETKINGTHGDAVTLEMDKSVEHEDLFYFD
                     ASNQMVGRTFDSCVNGVSLSFQPAPRKQGSVRLALAPTIRGERRRLEFTPSNQEYESP
                     FTEVTRIYDLNLRADVGDDSFLVVAPSEDASRSTSIGGQFFTTEDKAER"
BASE COUNT          325 a          540 c          595 g          267 t
ORIGIN      
        1 caccaagcgc tggaagaccg tgtcgatgaa taaatcgttc cacggccgat cgctggcgat
       61 gatcgccgcc accggaaatc ccgcggtgcg cgtcggcttc gaaccggagg tgccgggctt
      121 cacgcacgtc gatctgggtg acatggaagg cctcgccgcg gcggtcgatt cggaaacggc
      181 ggccgtcatc atcgaaccga ttcaaggcga aggcggcatc cacatcacgc cgaccgatta
      241 catcgtcgag ctgcggcggc tgtgcgatga gcgcaaggtc gcgctgatct tcgacgaagt
      301 gtggaccggc tgcgggcgca ccggcaaatg gttcgggcat cagtatttca ccacggccag
      361 cgggaagtgc gtcgagccgg acatcatgac gctcggcaaa gccgtcggcg gcggattgcc
      421 ggtcggcgtg atgtgggcca agccggaaat cgccaagctg ttcgtccccg gcaagcacgg
      481 cagcacgctg ggcggcaacc cgatctgcat ggcggtgtcg cgcacgatct tcgacgtgat
      541 cgagcgcgag cggttgactg ataatgccgc gacgctgggc gaatacggca tggcgcggct
      601 gaagagcgag aagcgcatcg cgcccaaaat caccgccgtg cgcggccgcg ggttgatgat
      661 cggcgtcgag ctgaaggaaa cccccgagaa gctggtcgag aaggggctgg cgaaaggcgt
      721 gttgctcaat ttgacgtcaa agaacgtgat ccgcctggcg ccgccgatca acatcacgcg
      781 cgaggactgg gaccgggggc tcgaccgcgt gatcgagacg atcgcgagct tataaacatg
      841 aggagagccg ggccgtcctc ggcccggacc ggcgcgggga cgggccggct ctgcttcgcg
      901 cgacgctcgc acgaacgcat cacgatgatg aagcgctgtt tgctttcgat cttcacgctg
      961 cacgccttgg tggggtgcca gtcgcatcgc gcgccaaccg ccgcgccgac cacgcggccg
     1021 gcgttgccga cgacgatgca gatctcgctg agcgcgcacc cgctgcagcc ggcggatccg
     1081 cgcgatccgc cgccggagct ttcgctgctg atccacctcg acatctatca actcgacgtg
     1141 ccggtcggcg cggtgagccg gaacgaagcg ctctggaagc gcgtgaacga acagacggtc
     1201 ggcgccggcg aaggcacgac ggacctgctg ttcaagaacg gcctgcgctg cggcgtggcg
     1261 ccgcgcagcg aatggtcgtt tttccgtgac ctgctcggcg ggcaaccggg ccggatgcgc
     1321 gagacgaaga tcaacggcac gcacggcgac gccgttacgc tggagatgga caagtcggtc
     1381 gagcacgaag acctgtttta tttcgatgcg tcgaaccaga tggtcggccg cacgttcgat
     1441 tcctgcgtga acggcgtgag cctgtcgttc cagcccgccc cgcgcaagca gggcagcgtg
     1501 cgactggcgc tggcgcccac gatccgcggc gaacgccgcc ggctggagtt cacgccgtcg
     1561 aaccaggagt acgagtcacc gttcacggag gtcacgcgaa tttacgactt gaacttgcgc
     1621 gccgatgtcg gggatgacag cttcctggtc gtcgcgccca gcgaagacgc gagccgatcg
     1681 acgagcatcg gcgggcagtt cttcacgacc gaagacaagg ccgagcg
//
LOCUS       DATIMH010000131         1895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5231556, whole genome shotgun sequence.
ACCESSION   DATIMH010000131 DATIMH010000000
VERSION     DATIMH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5231556"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VLI90_02165"
     CDS             <1..498
                     /locus_tag="VLI90_02165"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HSV13035.1"
                     /translation="DRRDLLDRQTAANDTLDRQRRLRPIWTEITHGGLKTDAAEADSQ
                     ARHAVLEWMQSAGVNLKAVNPERTTNVNQFQVISFHVTGNAHMYSLARLLWSLETATI
                     PVRVNDVQIKPQKEGTDDLTVDLSISTLCLPAATTPAGKTPATPPTPPAPTSESSASI
                     SWERS"
     gene            390..1352
                     /locus_tag="VLI90_02170"
     CDS             390..1352
                     /locus_tag="VLI90_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13036.1"
                     /translation="MPAGGDDAGGQDAGDTTNAARADQRVEREHQLGAVMKRLLTIGI
                     ITLSFALPPVFAQDQQQQQQQQEIDPAAAEAAMQAASAALEAAAAERAALEAQQQQMA
                     EQPGNGEPATQPVAAAEEQPEATAQPNGPTSRPTQQQQRNRFKRRTTTQPAVAHRPSP
                     PRPPRTVNTDFAMLLTRSIFKKGPQQIVDPGAPGSAPATRPAEVVAPEHNLVFNGVTE
                     ADAATALVEDTAGHKVNKLHVGDAIASGKVTGIDFDSLEYTVNGKARRIEIGQNLEGT
                     VAAPTSGPAVFGASGGTSSGDSGTSGGASNSILEKMRQRRMQGK"
     gene            1564..>1895
                     /locus_tag="VLI90_02175"
     CDS             1564..>1895
                     /locus_tag="VLI90_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13037.1"
                     /translation="MRRTLLLTPAVLLAVQPMLLLAQPAPAPAPPATQPSAVAAPTTE
                     PSPNTATVYRPPSTNRPATTQISFNFREAPLDVVLEHLSELAGFVVVKDASVSIDTRV
                     TVLSKQPVT"
BASE COUNT          392 a          625 c          639 g          239 t
ORIGIN      
        1 gatcggcgtg atttgctcga ccgccagacg gcggcgaatg acacgctgga ccgccagcgc
       61 cggcttcggc cgatctggac ggagatcacg cacggcggtc tgaagaccga cgccgctgag
      121 gccgacagcc aggctcgcca cgcggtgctc gagtggatgc aatccgccgg ggtgaacctg
      181 aaagcggtca atccggaacg cacgaccaac gtgaaccagt tccaggtgat cagcttccac
      241 gtgacgggca acgcgcacat gtattcgctg gcgcggctgc tgtggtcact ggaaacggcg
      301 acgatccccg tgcgcgtgaa cgacgtgcag atcaagccgc agaaggaagg cacggacgac
      361 ctgacggtgg acctgagcat ctcgacgtta tgcctgccgg cggcgacgac gccggcgggc
      421 aagacgccgg cgacaccacc aacgccgccc gcgccgacca gcgagtcgag cgcgagcatc
      481 agctgggagc ggtcatgaag cggttgctga cgatcggaat catcacgttg agcttcgcgc
      541 tgccgccggt gtttgcgcag gatcagcagc agcagcagca gcaacaggag atcgatcccg
      601 cggcggcgga agcggcgatg caggcggcgt cggcggcgct ggaagcggcg gcggcggaac
      661 gcgcggcgct cgaggcccag cagcagcaga tggccgagca gccgggcaat ggcgagccgg
      721 cgacgcagcc cgttgcggcg gccgaggaac aaccggaagc gactgcacaa cccaacgggc
      781 cgacgagccg gccaacgcag caacaacagc gcaatcgatt caagcgacgg acgacgacgc
      841 agccggcggt ggcgcatcga ccgtcgccgc cgcgcccgcc gcgcaccgtt aacaccgatt
      901 ttgcgatgct gctgacgcgc agcatcttca agaagggccc gcaacagatt gtcgatcccg
      961 gtgcgccggg ctccgcgccc gccacgcgac cggccgaagt ggtcgcgccg gagcacaacc
     1021 tggtattcaa cggcgtgacc gaggcggacg cggccaccgc actggtcgag gacacggccg
     1081 gtcacaaggt aaacaagctg cacgtgggcg atgcgatcgc ctcgggcaag gtgaccggca
     1141 tcgacttcga cagcctcgaa tacaccgtga acggcaaggc gaggcggatc gagatcgggc
     1201 agaacctgga aggcaccgtg gcagcgccga catcgggccc ggcagtcttc ggcgcctcgg
     1261 gcggcacgag cagcggcgac agcggcacga gcggcggtgc aagtaattcg atcctggaaa
     1321 aaatgcgcca acgccgcatg cagggcaagt gatgcagaat cgcagcgatg cgcgcggcac
     1381 caaaacggaa ccaccgggct aaccgcttca gcgcttagcg aaccatcgaa gcgcgccagt
     1441 aacacccgcg gtaatggggc atatttgtac ctggaccagc aagaccagtc tgatttcgcg
     1501 aacggagaac gaatgaaacg ctttaccatc aacgcgcgac gctcaacgcc agacaacgga
     1561 cggttgcgtc gtacgttgct tctgaccccc gcggttttgc tggccgtgca gccgatgctg
     1621 ctgctggccc aacccgcgcc cgcgccggcg ccaccggcca cgcagccgtc ggcggtggcg
     1681 gcgccgacca cggagcccag ccccaacacc gcaaccgttt atcgcccgcc gtccaccaac
     1741 cgcccggcga cgacgcagat ctcgttcaac tttcgcgaag caccgctgga cgtcgtgctg
     1801 gaacatctgt cggagctggc cgggttcgtc gtggtgaagg atgcatcggt gtcgatcgac
     1861 acgcgcgtga cggtgctgag caagcagccg gtcac
//
LOCUS       DATIMH010000132         4311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4942205, whole genome shotgun sequence.
ACCESSION   DATIMH010000132 DATIMH010000000
VERSION     DATIMH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4942205"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2496)
                     /locus_tag="VLI90_02180"
     CDS             complement(1..2496)
                     /locus_tag="VLI90_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HSV13038.1"
                     /translation="MPRDIPVGNGHLLVTFDNKYQMRDVYFPHVGQENHAGAGPCRFG
                     VHSDIPGTRKHGLFWTTSDGWTIRQRYLRDTLTTSVSLDHGGLRLALYCNDTVDFHRN
                     ILVRKIKVKNLASHERVVRIMHHQDFNMFGTKVGDTAYYDPELRSIVHYRGKRYLMMT
                     FYGNGEQRIDEYATGTSGFHGAEGTWRDAEDGHLQGNAIAQGAVDSTISHHVNLAPDG
                     ERTIYMVMMAGESREELLELHKWLTKMSPQGVIDRTTAYWRLWVGGTNINFGNLPPKV
                     VDLFKRSLLVVRTQIDNGGAIIAANDSDIMQFSRDTYSYVWPRDGALVADSLDLAGFP
                     DIARWFYNFCSKVITDDGYFYHKYNPDGSPASSWHPWVIKNARAMPIQEDETALVVWA
                     MWRHYYRYRDIEFVRPLWVDVVQKAADFMCRYRDPRTGLPHPSYDLWEERWGVHAFTV
                     ATVYGGLKAARNFAVCFGDREKAEKYNKAAEEIRAGAAKYLWSDKLKRFVRRLVPKEQ
                     PVPPDDTAYVESTELKSEEKTEDYYEVDEVIDASLYAIFKFHLFEADDERVEATMKQV
                     EEKLWVKTRVGGLARYENDYYHRVSNDIAAVPGNPWFICTLWLADYFITRAQTTAELK
                     MALPIFEWTASHSLESGVLAEQVNPYTNEPISVSPLTWSHATVVSTAIKYLEKLEQLQ
                     TCGACNQPIFRLRRMGPVEVKSQATFDRLEAEFEMAGTREMASAIGKFTKEDPRSKQV
                     LKATLAIDTRDCIGCDVCVAHCDKGVLKMVDGKALIDLRHLNKCDLDGECVEVCPTDV
                     VTLLVQPVEEAKPVGAMADDSPPDLEATPSAGV"
     gene            complement(2690..2824)
                     /locus_tag="VLI90_02185"
     CDS             complement(2690..2824)
                     /locus_tag="VLI90_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13039.1"
                     /translation="MQSIRVMLDRVIETLQLSRRNSRQRRARRTRAWERLFGLGAAVG
                     "
     gene            3041..3181
                     /locus_tag="VLI90_02190"
     CDS             3041..3181
                     /locus_tag="VLI90_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13040.1"
                     /translation="MSKVNLQKRQQVRQMELLPPRRMLPVILSRFVSDRLKKNGDGGD
                     CD"
     gene            complement(3413..>4311)
                     /locus_tag="VLI90_02195"
     CDS             complement(3413..>4311)
                     /locus_tag="VLI90_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HSV13041.1"
                     /translation="PSLVLPLALLLIFDAPTPADDSLVWPFLLDGGAASQPASPPATL
                     PSEQPQTAGQRTLAEFLGHFSGYEPMYFIAGPVDPMVKWQFSFKYRVLNPNAPLAEKF
                     PPLAGLHFAYTQMALWQIDKPSAPLFDTAYKPEFFWSNEDINYKIPGVAQWGFQGGYG
                     HESNGRGGPDSREINILFARPIVTFGDLEHFHVSLAPKFYTYIGSLYGNQDIWRYRGY
                     CDFRTIIGWRQGLELSTIGRVGNHWNRGSVQLDLTYPLRNLLANNFDVYLDAQYFYGY
                     GESLLTYNKRSSAFRIGLGLVR"
BASE COUNT          761 a         1323 c         1354 g          873 t
ORIGIN      
        1 tcatacgccc gccgaagggg tggcttctaa atccggcgga gaatcatcgg ccatagcccc
       61 tacgggcttc gcctcttcca ccggttgcac cagcagcgtc accacgtccg tcgggcagac
      121 ctcgacgcat tcgccgtcca ggtcgcactt gttgaggtgc cgcaggtcga tcagcgcttt
      181 gccatcaacc attttcaaca cgcccttgtc gcagtgggcg acgcagacgt cgcagccgat
      241 gcagtctcgg gtgtcgatcg ccagcgtggc cttcagcacc tgcttgctgc gcgggtcttc
      301 cttggtgaac ttgccgatgg cgctggccat ctcgcgcgtg ccggccattt cgaattcggc
      361 ttcgagacga tcgaacgtcg cctgcgattt gacctcgacc gggcccattc ggcggagccg
      421 aaagatcggt tggttgcacg cgccgcacgt ctgcagttgt tcgagtttct cgaggtactt
      481 gatggcggtg ctgaccaccg tcgcgtgcga ccaggtcagc gggctgacgc tgatcggttc
      541 gttcgtgtac gggttgacct gctcggcgag cacgccgctt tccagcgaat ggctcgcggt
      601 ccactcgaag atcggcaggg ccatcttcag ctccgccgtc gtctgcgcgc gggtgatgaa
      661 ataatccgcc agccagagcg tgcagatgaa ccacggattg cccggcacgg cggcgatgtc
      721 gttgctgacg cgatgatagt aatcgttctc gtaccgcgcc aagccgccca cgcgggtctt
      781 cacccagagc ttttcttcca cctgcttcat cgtcgcctcg acgcgctcgt cgtcggcttc
      841 gaagaggtgg aatttgaaga tcgcgtacaa cgacgcgtcg atcacctcgt ccacttcgta
      901 ataatcttcc gtcttctctt cgctcttcag ctccgtcgac tcgacatacg ccgtgtcgtc
      961 cggcggcacg ggctgttcct tcggcaccaa tcggcggacg aagcgcttca gtttgtcgct
     1021 ccacagatat tttgcggcgc cggcgcggat ctcctccgcg gccttgttgt acttctcggc
     1081 cttttcgcga tcgccgaagc aaacggcgaa attgcgcgcc gctttcaatc cgccatacac
     1141 cgtggcaacg gtgaacgcgt gcacgcccca gcgttcttcc cacaggtcgt aactcggatg
     1201 cggcaagccc gtgcgcggat cgcggtagcg gcacatgaaa tccgccgcct tctgcacgac
     1261 gtccacccac agcgggcgga cgaactcgat gtcgcgatag cggtagtagt gccgccacat
     1321 cgcccagaca accagggcgg tttcgtcttc ctgaatcggc atcgcccgcg cgtttttgat
     1381 cacccacggg tgccacgaag acgccggcga accgtcgggg ttgtacttgt gatagaaata
     1441 gccgtcgtcg gtgatcactt tactgcaaaa gttgtaaaac caacgagcga tgtcggggaa
     1501 gccggccagg tccagtgaat cggcaacgag cgcgccatcc cgcggccaga cgtacgaata
     1561 cgtgtcgcgc gagaactgca tgatgtcgct gtcgttggcc gcgatgatcg cgccgccgtt
     1621 gtcgatctgc gtgcgcacga ccagcagcga acgcttgaat agatcgacga ccttgggcgg
     1681 caggttgccg aagttgatgt tggtcccgcc cacccacagc cgccaatacg cggtggtgcg
     1741 atcgatcacg ccctgcgggc tcatcttcgt gagccacttg tgcagctcga gcagctcctc
     1801 gcgcgactcg ccggccatca tcaccatgta aatcgtgcgc tcgccatcgg gcgcgaggtt
     1861 cacgtgatgc gagatggttg aatcgaccgc gccctgcgcg atcgcgttgc cttgcagatg
     1921 tccatcttcc gcgtcgcgcc acgtgccctc ggccccgtgg aacccgctgg tgccggtcgc
     1981 gtattcgtcg atgcgctgct cgccgttgcc gtagaacgtc atcatcagat accgcttgcc
     2041 gcggtagtgg acgatcgagc gaagctcggg gtcgtagtac gcggtgtcgc ccaccttggt
     2101 gccgaacatg ttgaagtcct ggtggtgcat gatgcgcacg acgcgctcgt gcgatgccag
     2161 gttcttcacc ttgatcttgc ggaccaggat gttgcggtgg aagtcgacgg tgtcgttgca
     2221 gtacagcgcc aagcgaagcc cgccgtgatc gaggctgacg ctggtggtca gcgtgtcgcg
     2281 caggtagcgc tggcggatgg tccagccgtc ggacgtcgtc cagaacaggc cgtgcttgcg
     2341 cgtgccgggg atgtcgctgt gcaccccgaa gcggcagggg ccggcgccgg cgtggttctc
     2401 ctgcccaacg tgggggaaat acacgtcgcg catctggtat ttattatcga atgtcacgag
     2461 cagatgcccg ttaccgaccg gaatatcacg cggcatgcga ccgtcgcttt cgggtccaag
     2521 aaggaccaac aaattcacgc gtccctgcgt ctgggcgagc ggttgactgc ggtcgcccgt
     2581 ttatatgctg gccgaggggc cgtcacaagt ttctgtgggg agagccgggc cgtcttcggc
     2641 tcggattgtt cccgaagatt ccgggccgag gacggcccgg ctctcctggt tatcccacgg
     2701 cggcgccgag gccgaagagg cgttcccacg cgcgtgttcg gcgcgcgcgg cgctggcggc
     2761 tgttgcgccg cgataattga agcgtttcga tcacacgatc gagcatcacg cgaatgcttt
     2821 gcatttttct gccttccctt ccttggtccg ctcggggcgg ggtgggcatg cgcgcaggta
     2881 cttcgcgcgc atgccgctgc caccaccgca tcaggaatat cgagcgcttg atcgcaacac
     2941 tgaatcagtt ggcgggttta tgccgacttg gcggcgccaa tgttccgata atagatcgtg
     3001 cgatttgcgg gcgataacat gtggcgcgtc actgcctgcg atgtccaaag taaaccttca
     3061 aaagcgacaa caggttcgtc aaatggagct gctgccgccg cggcggatgt tgccggtgat
     3121 cctgtcgcgg tttgtgtcag accggttgaa gaagaacggc gacggcggcg attgcgactg
     3181 atgcacaccg ccgccgcttg cggcttcgca atcccaggcg gcatggtctg ctaagccgca
     3241 agcggcgggg tgattcaaaa caatcgtccg tcctataacc gacctcgccc gcggatggtg
     3301 aatctatctg tggaacagga ggtcgccatg gaacagcaga tacatacgca tgccctttgg
     3361 ccgacgtttc tcgcgctggg attgctcatc gccatattgg caacgatgta attcatcgca
     3421 ccagccccag accgatgcgg aaggcgctgg agcgcttgtt gtacgtcagc aggctttcgc
     3481 cgtagccgta gaaatactgg gcgtcgaggt acacgtcgaa attgttggcc agcagattgc
     3541 gcagcgggta ggtcaggtcc aactggacgc tgccgcggtt ccagtggttg ccgacccggc
     3601 cgatcgtgga cagctccagc ccctgccgcc aaccgatgat cgtgcggaag tcgcagtacc
     3661 cgcggtagcg ccagatgtct tggttgccgt agagggaacc gatgtaggtg tagaacttgg
     3721 gcgcgaggga aacgtggaag tgctcgaggt cgccgaacgt gacgatcggc cgggcgaaga
     3781 ggatgttgat ctcgcgcgag tctggcccgc ctcggccgtt ggattcgtgc ccgtagccgc
     3841 cctgaaatcc ccactgcgcc acgcccggga ttttgtagtt gatgtcctcg ttggaccaga
     3901 agaactccgg cttgtacgcc gtgtcgaaga gcggcgcgga tggcttgtcg atctgccaga
     3961 gcgccatctg tgtgtacgca aaatgcaacc ccgcgagcgg tggaaatttc tccgccagcg
     4021 gcgcgttggg attcagcacc cgatacttga agctgaactg ccacttcacc atcgggtcca
     4081 ccggcccggc gatgaagtac atcggctcgt agccggagaa gtgcccgagg aattcggcta
     4141 gcgtccgctg ccccgcggtc tgcggctgct cgctcggcaa cgtcgccggt ggcgacgcag
     4201 gctgcgatgc agcgccgccg tcgagcagaa acggccaaac cagcgaatcg tccgcaggtg
     4261 tcggtgcgtc gaagatcagc aaaagtgcca agggaaggac gaggctcgga a
//
LOCUS       DATIMH010000133         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5520560, whole genome shotgun sequence.
ACCESSION   DATIMH010000133 DATIMH010000000
VERSION     DATIMH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5520560"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VLI90_02200"
     CDS             <1..877
                     /locus_tag="VLI90_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13042.1"
                     /translation="AVGYQNLFDESTWIVKRRLLDGAIGDPTCASVVCCWPRSDAYFS
                     RTPWAGKLKRNGTWVLDSPANNAMAHFLNLALFLLGPTERESAEPLAVEAELYRVNDI
                     ENYDTCCLRYELPRGGKLVIGFTHACRRTIHPAITITGSNGSMKFWNSNRYEITTNNN
                     GGPHSYPAAPHVGSLVTRGFADYVLGSPSPHVATLTNARAHTIAVNGASEAAPVRAIP
                     SEFIKVVPGPDGHPLRTLPGIEDALLACAGECQMLHESARAVWSVPAGSKDLRGYDHF
                     TAPAGGRTAPVRELV"
     gene            complement(928..1608)
                     /locus_tag="VLI90_02205"
     CDS             complement(928..1608)
                     /locus_tag="VLI90_02205"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSV13043.1"
                     /translation="MPRVSLAVLVVLFSVVQLRADQPATAPAISGTLGAPIQLFNGKD
                     IDSWKWVARPPKAGTTQPVDLASPFAVRDGLLHSNGKPVGYLRTPDVYDNYILTVEQR
                     HVTKGNGGVLFAISGPDKVWPHCLEAQGQNGEEGDIRNVADFELNMDPSRLEPKRLRR
                     IGPSSEKPVGEWETIEVIVDHGNLTLKVNGQLQNLATYSEELAGYIGLQAEGAEMEFR
                     KVELTPIE"
     gene            complement(1694..2065)
                     /locus_tag="VLI90_02210"
     CDS             complement(1694..2065)
                     /locus_tag="VLI90_02210"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSV13044.1"
                     /translation="MSSSHDLQSESNKAAAERRRRERVAGETQGWILSAMPQLPAHLP
                     DGDDEAWEVRIHNVSRIGVGFASTAAMRIGDEHRIRIGRGPMKRARLIRVVACRQGDD
                     GTYTVGAEFVDHPSKTLARAS"
     gene            complement(2122..2490)
                     /locus_tag="VLI90_02215"
     CDS             complement(2122..2490)
                     /locus_tag="VLI90_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13045.1"
                     /translation="MLNTPLPRDLNVLPSATWRERRRRRRLTVVTSASLRPVAQHVFD
                     ATDEGPQDAVAEQLVIVHDVSLDGLGLRSRLPLDVCAQYRIHCEDRNVFLRNSRLRVV
                     SCRRGRDGLYEIGAQLCNGR"
BASE COUNT          438 a          921 c          837 g          476 t
ORIGIN      
        1 ggcggtgggt tatcagaatc tgttcgatga atcgacgtgg atcgtgaagc ggcggttgct
       61 cgacggcgcg atcggtgatc cgacctgcgc cagcgtcgtc tgctgctggc cgcgcagcga
      121 tgcgtacttc agccgaacgc cgtgggcggg gaagttgaag cgcaacggca cgtgggtgct
      181 cgacagcccg gccaacaacg cgatggcgca ctttctgaac ctggcgctgt tcctgctcgg
      241 tccgaccgag cgcgaatccg ccgagccgct ggcggtcgaa gctgagcttt atcgcgtcaa
      301 cgacatcgaa aattacgaca cctgctgcct gcgctatgaa ctgccgcgcg gcgggaagct
      361 ggtcatcggt ttcacccacg cgtgccggcg gacgattcac ccggccatca cgatcaccgg
      421 cagcaacggg tcgatgaagt tctggaactc caaccgctac gagatcacga ccaacaacaa
      481 cggcggcccg cacagttacc cggccgcccc gcacgtgggt tcgctcgtca cgcgcgggtt
      541 cgccgactac gtcttgggat caccctcgcc gcacgtggcg acgctgacca acgcgcgggc
      601 gcacacgatc gccgtcaacg gcgcctccga agcagcgccc gttcgcgcca ttccatctga
      661 gttcatcaaa gtggtgcctg gccccgacgg gcatccgctg cggacattgc ccggcatcga
      721 agatgcgctg ctggcgtgcg ctggcgagtg ccagatgctg cacgaatcgg ccagagccgt
      781 ctggtccgtc ccggccggca gcaaagatct gcgcggctac gaccacttca ccgcccccgc
      841 cggcggtcgc accgcgccgg tgcgcgaact ggtttaaacc tcgaatccgc cgcgtagtaa
      901 tgccgtagca caggctcata gcccgggcta ctcgattggc gtcagctcca ccttgcgaaa
      961 ctccatctcc gcgccctcgg cctgcaggcc gatgtagccc gccagttctt cgctatacgt
     1021 cgccaagttc tgcagctgcc cgttcacctt cagcgtcagg ttgccgtgat cgacgatcac
     1081 ctcgatcgtc tcccactcgc cgaccggctt ctcggagctt ggcccgattc gccgaagccg
     1141 cttcggctcg aggcgcgatg ggtccatgtt caactcgaaa tccgcgacat tgcggatgtc
     1201 gccctcttcg ccgttctgtc cctgcgcttc caggcagtgc ggccaaactt tatcgggccc
     1261 gctgatcgcg aacagaacac cgccgttgcc cttggtcacg tgacgctgct cgacggtgag
     1321 gatgtagttg tcgtaaacgt ccggcgtgcg cagatatccg acgggcttgc cgttcgaatg
     1381 gagcagccca tcgcgcaccg cgaagggact ggccaaatcc accggctgcg tcgtccccgc
     1441 cttgggcggg cgcgcgaccc acttccagct gtcgatgtcc ttgccgttga agagttggat
     1501 cggcgcgccg agcgttccag agatcgccgg cgccgtcgcc ggttgatccg cgcgcaactg
     1561 cacgacgctg aacagcacca ccagtacagc gagagaaacg cgcggcatcc tcatgcccgc
     1621 tgatacccgc tgcgatgtga tttgttgcga tggaggctgg cccgctccag tgggtcgggc
     1681 gctcggcgcg cgatcaactc gcgcgggcga gcgtcttgct cgggtgatcc acgaactccg
     1741 ctcccacggt gtacgtgcca tcgtcccctt gccggcacgc gacgacgcgg atgagccgtg
     1801 cccgcttcat cggaccgcgg ccgatgcgaa tgcgatgttc gtcgccgatg cgcatggccg
     1861 ccgtcgaggc gaagcccacg ccgatgcgcg agacgttgtg aatccgcact tcccaggctt
     1921 cgtcatcgcc atccggcaaa tgcgccggca gctgcggcat ggccgacagg atccacccct
     1981 gcgtctcgcc cgccacccgc tcgcgccgcc ggcgttccgc cgcggctttg ttcgactcgc
     2041 tttgtaaatc gtggctgctg gacatgcgtt ccttatcggc agagccggcg gcgagcggcg
     2101 atttgttttt cgcgccgcgc tttatcggcc gttgcacagc tgcgcgccga tctcatacag
     2161 cccgtcgcgc ccgcggcggc aggacacgac gcgcagccgc gagttgcgca gaaagacatt
     2221 gcggtcttcg cagtggatcc gatattgcgc gcacacgtcg agcggcaaac gcgatcgtaa
     2281 acccagcccg tcgagcgaga cgtcgtgcac gatcaccagc tgctcggcca ccgcatcttg
     2341 cgggccttcg tcagtggcat cgaacacatg ctgcgccacc ggccgaagtg acgcggaagt
     2401 gacgacggtc aaccgccggc ggcgtcgccg ctcgcgccac gttgccgacg gcagcacatt
     2461 caaatcgcga ggcagcgggg tgttgagcat accccgcgta tcggcgcgtt ccggggcgcg
     2521 gttaagatgt gccggttgtg cgggggcggc gaaaacatct cagtgcgtcg cgccctcttt
     2581 gatagccgcg ggcgacagcc ggcgggggct ttcgaacacc agcccggttg acgttcccaa
     2641 gcccccgctg gctgtcgcct gcggctatca cg
//
LOCUS       DATIMH010000134         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1477266, whole genome shotgun sequence.
ACCESSION   DATIMH010000134 DATIMH010000000
VERSION     DATIMH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477266"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VLI90_02220"
     CDS             complement(<1..196)
                     /locus_tag="VLI90_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV13046.1"
                     /translation="MTRRAFTLLEMLLATALAAVLMLAVLTMLAGVSRDGHRVRAAAT
                     SASPAQQAITERVRWDIVNAQ"
     gene            complement(193..537)
                     /locus_tag="VLI90_02225"
     CDS             complement(193..537)
                     /locus_tag="VLI90_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13047.1"
                     /translation="MLAGLVVLGTLLVSVAIARARFVHQAADAERRLSATRATSAMLA
                     TWFTGPTPAVPLAAEGALDGAANFTWQTHVMPKRPAAELTAVIVRLDVFDRSAPSSTP
                     VLSVDLLMHKNP"
     gene            complement(539..1063)
                     /locus_tag="VLI90_02230"
     CDS             complement(539..1063)
                     /locus_tag="VLI90_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV13048.1"
                     /translation="MTRACPRAFTLIELMVAVLLLALLASAAALSFSQPIRAARSQDA
                     IDQLRTFDATVRQAAVAAGRATRMVLDLRTGTIARSDGGTERYRTTLPPGYRVDQVRI
                     GDRTVDTGQALIDVSPLGISRTYALRLHGPTVDQWLLFAGLSGQLTLVIDESNLDAIF
                     AQIAPPSAARHDAD"
     gene            complement(1060..1668)
                     /gene="gspG"
                     /locus_tag="VLI90_02235"
     CDS             complement(1060..1668)
                     /gene="gspG"
                     /locus_tag="VLI90_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HSV13049.1"
                     /translation="MQFSNDASDPDRPSGNRASGEPSGSAAGRESSNRRSRDRHTSTP
                     RAPRGGAAGLSAGAGSQAPFAQRAQHSSPAFSLVEIMIVVVIIGLMAGLVTYATSGYL
                     DRAKRQRARSDIATYSGAVDAYYLAKGSYLDNQTGLKALVPEFIKVLQPDPWGRPYQY
                     VAPGKGGPYDIISYGADGRDGGSGADADISNWDVEVAEIKHK"
     gene            complement(1655..2872)
                     /locus_tag="VLI90_02240"
     CDS             complement(1655..2872)
                     /locus_tag="VLI90_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSV13050.1"
                     /translation="MAVFTYKAIATGAADDVSGTIAADTPRHARDLLREQGLIVRDLA
                     DYKSGQSRSRSFRPARPRRGARRLTTTFIRELATLLGVGAPLLESLETIARQHAGRFQ
                     SSIILLRDRVAAGASLAAAMREQPRVFDDLCVNITEVGEDAGTLDASLERLAEFRERT
                     DQLRNRVGTALIYPAIVSTLAVFSSVFLMTFVVPKILQPLIEQDLPLPLPTRIVKAAS
                     DFALDWWWVLVLIVGAIGVTVASILRTRGGRRALDRTVLRLPLLGDLVRKQAIVRIAV
                     VLSTLLKSGIVFVRALQIAQRSTGNQILRDALNRCEIAITAGGDIGDAMEQTAAFPPM
                     VVQVFALGQQSGRLEEMLDRLAAAYEQQVNTAAQRLAAILEPALIVALALFVLFIVLA
                     TVLPILEVGNAVQ"
BASE COUNT          508 a          964 c         1024 g          467 t
ORIGIN      
        1 actgcgcgtt gacgatgtcc catcgcacgc gctcggtgat cgcctgctgc gcgggggaag
       61 cgctggtggc tgcggcgcgg acgcggtggc cgtcgcgcga cacgccggcc agcatcgtca
      121 acaccgccag catcagcacc gcggcgagcg cggtggccag cagcatctcc agcaatgtga
      181 acgcccggcg ggtcatgggt ttttgtgcat gagcaaatcg accgacaaga ccggggtact
      241 cgacggcgcc gaccggtcga acacatcgag ccgcacgatg acggcggtca gttccgccgc
      301 cggtcgcttt ggcatcacgt gcgtttgcca ggtgaagttg gcggcgccgt ccagcgcgcc
      361 ctccgcggcc agcggcaccg cgggcgtggg gccagtgaac catgtggcca gcatcgcact
      421 ggtggcgcgg gttgcgctga gccggcgctc ggcgtcggcg gcttggtgga cgaagcgcgc
      481 gcgggcgatc gccaccgaaa ccagcagcgt gcccagcacg acgaggccgg ccagcacctc
      541 aatcagcgtc gtgccgagcc gcgctcggcg gcgcgatctg cgcgaagatg gcgtcgaggt
      601 tcgattcatc tataaccagc gtcagttgcc cgctcaggcc ggcgaacagc agccattgat
      661 ccaccgtggg cccgtgaaga cgcagcgcgt acgtccggct gatgcccagc ggcgacacgt
      721 cgatcagcgc ctgtcccgtg tcaacggtgc gatctccaat tcgcacctgg tccacgcgat
      781 atcccggcgg cagcgttgtc cgataccgct cggtgccgcc gtcgctgcgc gcgatcgtgc
      841 cggtgcgaag atcgaggacc atccgggtag cccgccccgc ggccaccgcc gcctggcgca
      901 ccgtggcgtc gaacgtgcgg agttgatcga tcgcatcttg cgatcgggcg gctcgaatcg
      961 gctgcgagaa actcagtgcc gccgccgacg ccagcagcgc cagcagcaga accgccacca
     1021 ttagctcgat cagcgtgaac gctcgtgggc atgcgcgtgt cacttgtgct tgatctccgc
     1081 gacttcgacg tcccagttgg agatgtcggc atcggcgccg ctgccgccgt cgcggccgtc
     1141 ggcgccatag ctgatgatgt cgtacggccc gcccttgccc ggggcgacgt actgatacgg
     1201 ccggccccac gggtcgggtt gaagcacctt aatgaactcc ggcaccagcg ccttcagccc
     1261 ggtctggttg tcgagatagc tgcccttggc caggtagtat gcatccaccg cgccggaata
     1321 ggtggcgatg tcggagcgcg cccgctgccg cttggcgcga tcgaggtacc cgctggtggc
     1381 gtaggtgacc aatcccgcca tcaggccgat gatcacgacg acgatcatga tctcgacgag
     1441 tgagaaggcg ggggaactgt gttgtgcgcg ctgtgcgaag ggcgcttgcg atcccgcgcc
     1501 agcggagagc ccagcggctc cgccgcgggg cgcgcggggg gtcgaggtat gtcgatcgcg
     1561 cgaacggcgg ttacttgatt cccgccccgc ggccgagccg ctgggctctc cgctggcgcg
     1621 gtttccggat gggcggtctg gatccgaagc atcgttactg aactgcattg cccacctcca
     1681 ggattggcag cacggtggcc aggacgatga acaacacgaa cagcgccagt gcgacgatga
     1741 gcgcgggttc taatatcgcc gccagccgct gggcggcagt gttcacctgc tgctcgtacg
     1801 cggcggccag gcggtcgagc atctcttcca gccggccgga ctgctggccc agcgcgaaga
     1861 cctgcacgac catcggcggg aaggccgcgg tctgctccat cgcgtcaccg atgtcaccgc
     1921 cggcggtgat ggcgatttcg cagcggttca acgcgtcgcg caggatctgg ttcccggtgc
     1981 tgcgctgggc gatctgcagt gcgcggacga agacgatgcc gcttttaagc aaggtgctga
     2041 ggacgacggc gatgcggacg atcgcctgct ttcgaacgag gtcgccgagc aacggaaggc
     2101 gcagcactgt gcgatccaac gcgcggcggc cgccgcgcgt gcggaggatg gacgcgacag
     2161 tcacgccgat cgcgcccaca atcagcacga gcacccacca ccagtccagc gcgaaatcgc
     2221 tggcagcctt aacgatgcgg gtcggcagcg gcagcggcag gtcctgctcg atcagtggct
     2281 gcaggatctt cggcacgacg aacgtcatga gaaagacgct ggagaagact gcgagcgtcg
     2341 agacgatcgc ggggtaaatc agcgcggtgc cgacgcggtt gcgcagttga tcggtgcgct
     2401 cgcgaaattc ggccagccgt tccagcgacg cgtccaacgt gccggcgtct tcgccgacct
     2461 cggtgatgtt gacgcacaag tcgtcaaaga cgcgcggctg ctcgcgcatc gcggcagcga
     2521 ggctcgcccc ggcggcgacg cgatcgcgca gcaggatgat cgacgattga aaccgcccgg
     2581 cgtgttggcg ggcgatggtc tccaatgatt cgagcagcgg agcgccaacg cccagcagcg
     2641 tggccagctc gcggatgaag gtcgtggtca atcggcgcgc cccgcgtctc ggccgggccg
     2701 gcctgaacga tcgcgatcgt gattgcccgg atttgtaatc ggcgagatcg cgaacgatca
     2761 gcccctgctc gcgcagcagg tcgcgcgcgt gccgcggggt gtccgcggcg atcgtgccgc
     2821 tgacatcatc agccgcgccg gtggcgattg ctttataggt gaagacggcc atggagtctc
     2881 aagaaacagt gggcgaatga ccaatgtcca agcagcaaat cccaataaaa tcccaatgac
     2941 cgaggccgaa cggccgaatg gct
//
LOCUS       DATIMH010000135         5999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3787394, whole genome shotgun sequence.
ACCESSION   DATIMH010000135 DATIMH010000000
VERSION     DATIMH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3787394"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VLI90_02245"
     CDS             <1..232
                     /locus_tag="VLI90_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13051.1"
                     /translation="EAAPPVGLEYAMALYKIPKNLGKVRVAMFLGGKFAVWNGKTGTG
                     EFRILCKDRKQAEEVAGKINRKEHDGAIEVLG"
     gene            347..1255
                     /locus_tag="VLI90_02250"
     CDS             347..1255
                     /locus_tag="VLI90_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSV13052.1"
                     /translation="MTVTATPTRRPDLLLSPEFMARLDQLDVMSRKLLAGKMKGERRS
                     KRRGQSVEFADYRNYVIGDDLRFIDWNIYARLDKLFLKLFLEEEDLSLYILCDVSKSC
                     DFGNPNKAFYIKQVAAALGYVGLVNYNRVTIAAMNNTVVADTGALRGRTKVNQMMDFV
                     SKLQAEGPSDLAAACKRFALANRRKGVCVVLSDFFDKGGYENGLRYVAGGRYDLFCVQ
                     TLAPQEIEPDLQGDLKLKDIEDNDEAEVSITQPLIKQYKANLNAYCLALKDYITRRGG
                     TYLFTSTAVPFDTLVLNYLRERGLLG"
     gene            complement(1256..1540)
                     /locus_tag="VLI90_02255"
     CDS             complement(1256..1540)
                     /locus_tag="VLI90_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSV13053.1"
                     /translation="MECLVAPLRVADAVLVAAYDGLFRSEELMAAGIHLDILREAARL
                     RATFPGVRTPDAIHTATARMHNCVMFLTNDAGFRRIPGLPVVLLDDVVKS"
     gene            complement(1540..1701)
                     /locus_tag="VLI90_02260"
     CDS             complement(1540..1701)
                     /locus_tag="VLI90_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13054.1"
                     /translation="MGSLTLPANGTVYLDTMTVICSIETQPVYWPILKPLWIAARDGK
                     AALMTSELT"
     gene            complement(1689..1928)
                     /locus_tag="VLI90_02265"
     CDS             complement(1689..1928)
                     /locus_tag="VLI90_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13055.1"
                     /translation="MQNALHIRTQVLPGRKVEVTAAELQEGETVEVFVIRPTSVHASR
                     PSALQLIESLNGHRHFPNAADVDRHLRQERDSWDH"
     gene            2144..3310
                     /locus_tag="VLI90_02270"
     CDS             2144..3310
                     /locus_tag="VLI90_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSV13056.1"
                     /translation="MAISRPSALLRLILMWIACGAAGCATSPPPPASRMVEPANATSL
                     VQTIANAKPGDTIRVAAGTYALSEPLRLTTKGDVDHPIRFESVGPGRAVLDFAAEAEV
                     KVNAGVAITGDYWQLTGIEVAHAGSYGFNITGSHNGLLHCVSRENRNSGTQIEAGGSY
                     NVIDDCESFRNFDLKTKGEDADGFTAKHSVGPGNVFRHCRSYQNADDGWDLWMSPNPI
                     VVEDCVSFRNGYNIWNFPDFQGDGNGFKFGGNYVATAHIARRCVSIENPLHGFDQNHN
                     IGPLTLEDCVAIRCGKGFCFPELPRQGKVILRRCTSFGCVNVLEPEVVTEECRWYADI
                     PTGTLGPPPRPGHRNVKGAGDVPTTEPTPMYLPQGAPLWGRPSDTPATQPYPEP"
     gene            3353..4162
                     /locus_tag="VLI90_02275"
     CDS             3353..4162
                     /locus_tag="VLI90_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HSV13057.1"
                     /translation="MADRSPFPGMDPWLESYWGDIHATIITDFADQIATQLPPELIVR
                     VEEMVYVIDPIGDRGRVVKPDVARFDLGGARSPAAQQSGTAVAEPIRIMIPDESVTEG
                     FIEIRELRQGHPLVTAIEVLSPTNKCSTMGRSEYLRKRDGYYQAKINVIELDLIRAGR
                     HLVGVPVERVQGSLDAPYKCVVRHGGAMAGVGVDYYPLRLRERLPRIAIPLRPADPSA
                     VIDLQRPIDHAYQRGSYCRVIDYNQPPDPPLSPDDAAWAAERIAGARAGNR"
     gene            4208..>5999
                     /locus_tag="VLI90_02280"
     CDS             4208..>5999
                     /locus_tag="VLI90_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSV13058.1"
                     /translation="MTWFPHFLSPLSAAIAAAIAIPALLILYFLKLRRREMPVSSTFL
                     WKKAVQDLQVNAPFQRLRRNLLLFLQLLLLLMLLLALSRPVANFTPGAGKLSVILIDR
                     SASMSAADEAGGKTRLDEAKRRAKELVSSLERNGQATIIAFDDTPEIMQTFTGDAQAL
                     RNAIDRIAPTDRKSRLKMAFQLADAQASYIPEQNRSNVKPDVRLYSDGRVLDANELLL
                     HGDLKYEKIGTDSASNIAIVALDAKRNYELPTQVQVFARLANFGVQPKTADVQLTIDG
                     ALRSIAAVSLPPDRWNDPNWVKDHPGEKDDTFQAKDSVEFTIDLPTAGVIKVEQMSKQ
                     GDLLSADDSAQVVVPPPKSLSVALVTTENYYLDRAIQSMNLKDPVRLTPDEYEQKHPD
                     KYDVIIFDRYYPTWTPDAGNFIYVGCAPTAANAKVKAVKENEHYVVEDDVGVLDWQRD
                     HPILRHLNLSKVFAAHALKLTLPPDAQTLIDGTKGPMVVLSRDDRTTNLIVTFDLLDS
                     NWPLKLSFPIFLHNALQFLALGSDMNVRPSYEPGATPKLPRAKLEEARHGLTSLALDG
                     PTGRISVPVPPTGDFALPALNRVGLYTTDPP"
BASE COUNT         1247 a         1864 c         1836 g         1052 t
ORIGIN      
        1 tgaagcggca ccgccggtcg ggcttgaata tgctatggcc ctttacaaga tccccaaaaa
       61 cctcggcaaa gtgcgtgtgg cgatgttcct cggcggcaaa ttcgcggtct ggaacggcaa
      121 gaccggcacg ggcgagttcc gcatcttgtg caaagatcga aagcaggcgg aagaggtcgc
      181 gggtaagatc aatcgcaagg aacacgatgg ggcgatcgag gtgttgggat gactaccaaa
      241 caaaagccaa cggctttgcc gtgggacggg cttcttagaa acgggtccca cggcagagcc
      301 gtgggctttc ttgagagagc gaaaacactc tgacattgaa aatcgaatga ccgtcaccgc
      361 gacaccaact cgacgaccgg atttgctcct ctcccccgag ttcatggctc ggctggacca
      421 gctggacgtc atgagccgaa agctgctcgc cgggaagatg aagggcgaac gccgcagcaa
      481 gcggcggggg cagtcggtgg aattcgccga ctaccgcaac tacgtcattg gcgacgacct
      541 ccgcttcatc gactggaaca tctacgcgcg gctcgacaag ctctttctaa aactcttcct
      601 cgaagaggaa gacctctcgc tctacattct gtgcgacgtc agcaaaagct gcgacttcgg
      661 caatcccaac aaggcgttct acatcaagca ggtggcggcc gccctgggct acgtcggctt
      721 ggtcaactac aaccgggtga caatcgcggc catgaacaac acggtggtgg ccgacaccgg
      781 tgcgctgcgc ggacgcacga aggtcaacca gatgatggac ttcgtcagca aactgcaggc
      841 ggaggggccg agtgatctgg ccgccgcgtg taagcgcttc gcgctggcca accgccgcaa
      901 gggtgtgtgc gttgtgctca gcgacttctt cgacaagggc ggttacgaga acggcctccg
      961 ctacgtcgcc ggcgggcgct acgacctgtt ctgtgtgcag acgctcgcgc cgcaggaaat
     1021 cgaacctgac ctgcagggcg atctgaaact caaagatatc gaagacaacg acgaagccga
     1081 ggtgtcgatc acgcagccgc tgatcaagca gtacaaggcg aatttgaacg cgtattgcct
     1141 ggcgctgaaa gactacatca cccgccgtgg cgggacgtat ctgttcacca gtaccgcggt
     1201 gccgtttgat acgctggtgc tgaactacct gcgcgagcgc gggctgctgg gatgatcagg
     1261 atttaaccac atcatcgagc aagacaacgg gcagaccagg aattcgacga aagccggcat
     1321 cattggtcaa aaacatgacg caattatgca ttcgagctgt tgctgtatga atggcgtccg
     1381 gcgtccgtac gccgggaaac gtcgcgcgca aacgagcagc ttcgcgaaga atatcgagat
     1441 ggatgccggc cgccatcaac tcctcagagc ggaacaatcc gtcgtacgct gcgaccagaa
     1501 cggcatctgc aacacgcagc ggcgcgacca agcattccat caggtcagtt cgctggtcat
     1561 caacgcggcc ttaccgtcac gagctgcgat ccagagcggt ttgaggatcg gccaatacac
     1621 aggctgtgtc tcaatgctgc agatcaccgt catcgtatca agataaacgg ttccgttggc
     1681 gggcagggtc aatgatccca tgagtcccgc tcctggcgaa ggtgacgatc cacgtcggca
     1741 gcattgggaa aatgccggtg gccattgagc gactcgatca gttgcagcgc ggaggggcgg
     1801 gatgcatgca ccgaggtagg ccgaatgacg aagacctcca cggtttcacc ctcttgaagt
     1861 tccgccgccg taacttccac cttacggccc ggcaggacct gcgtgcgaat atggagggcg
     1921 ttctgcatgc ctcgatcata ccacggcgca ggcgggtagg tgagcgttgc tcaacacacc
     1981 gcgttcttgt tgcacggatc gcccgcggac atctttaccc tttcggttaa atctcttccc
     2041 tttccaccgc cgcggcaatg cgtgccttgg cggtcgcgcg cggccgtcat aagctgacgc
     2101 gcgccgtcag gatgcaaccc gcgcgcggat aaggaacaac tcgatggcca tctctcgacc
     2161 aagtgcactg cttagattga tattgatgtg gatcgcctgc ggcgccgcgg gttgtgcgac
     2221 ttcgccgccg ccgccggcaa gccggatggt tgaacctgcg aatgcgacgt cgctggtcca
     2281 aaccatcgcg aacgcaaaac cgggcgacac catccgtgtt gccgcgggaa catacgcgct
     2341 cagcgagcca ttgcgactaa cgacgaaagg cgatgtggat catccgatcc gcttcgaaag
     2401 cgttggtccc ggtcgcgcgg ttctcgactt cgccgccgag gcggaagtga aggtcaacgc
     2461 cggcgtcgcg atcaccggcg actattggca actgaccggc atcgaagtcg cccacgccgg
     2521 aagttacggg ttcaacatca ccggctcgca caacggcttg ctgcactgcg tctcgcgcga
     2581 gaaccgcaac agcggcacgc aaatcgaagc gggcggaagt tacaacgtca ttgacgactg
     2641 cgaatcgttc cgcaatttcg acctcaagac caagggggaa gacgccgacg gcttcaccgc
     2701 aaaacactcg gtgggaccgg ggaacgtctt tcggcattgc cggtcatatc agaacgccga
     2761 cgatggatgg gatttgtgga tgagcccaaa cccgatcgtc gtcgaagact gcgtgtcgtt
     2821 ccgcaacggc tacaatatct ggaacttccc cgattttcag ggcgatggca acggcttcaa
     2881 gttcggcggg aactacgtcg ccaccgcgca catcgcccgg cgatgcgtgt cgatcgaaaa
     2941 cccgctgcac gggttcgacc agaaccacaa catcggcccg ctgacgctgg aagactgcgt
     3001 cgccatccgc tgcggcaagg gcttttgttt ccccgaactg ccgcggcagg ggaaggtcat
     3061 cctgcgtcgc tgcacatcgt tcggctgcgt gaacgtgctg gagccggagg tggtgacgga
     3121 ggagtgccgc tggtacgccg acatccccac cggcacgctc ggcccgcccc cgcggccggg
     3181 gcaccgcaac gtcaaaggcg ccggcgacgt gccgacgacc gagccgacgc ccatgtacct
     3241 gccacagggc gcgccgctat ggggtcgccc gagcgacacc ccggcgacgc agccgtatcc
     3301 ggagccgtaa caatcgcgag tttccctccg ggttcggata taatccccag caatggccga
     3361 tcgaagccca tttccgggga tggacccgtg gcttgaaagc tactggggcg acatccacgc
     3421 gacgatcatc accgatttcg cggaccagat cgccacgcaa ctgccgcccg agttgatcgt
     3481 gcgcgtcgag gagatggtgt acgtcattga tccaatcggc gaccgcgggc gcgtcgtcaa
     3541 gccggacgtt gctaggtttg acttgggcgg tgcgcgatcg ccagcggcgc agcagtccgg
     3601 tactgcagtg gccgagccca tccgcatcat gattcccgat gaatcggtca cagagggatt
     3661 catcgaaatc cgtgaactgc gtcaggggca tccgctggtg accgcgattg aagttctcag
     3721 cccgacgaac aagtgctcga cgatgggtcg atccgaatac ctgcgcaagc gggatggata
     3781 ctaccaggcg aaaatcaacg tgattgaatt ggatctgata cgcgcgggtc ggcacttggt
     3841 tggtgtgccg gtcgagcgcg tgcagggatc attggacgcg ccgtacaagt gcgtcgttcg
     3901 gcatggcggc gcgatggcgg gcgtgggtgt cgattactat ccgcttcgtc tgcgcgaacg
     3961 attgccgcga attgcgatcc cattgcgacc ggcggatccc tcggcggtga tcgaccttca
     4021 acgcccgatt gatcatgcgt atcagcgcgg cagttattgt cgggtgatcg actacaatca
     4081 gccgcccgat ccaccgcttt cgccggatga cgcggcgtgg gcggcggaac gaatcgctgg
     4141 tgcgcgagcc ggcaacagat gaatggtgcc cccatctgct ccgtcataat tgagtagcag
     4201 ccaacgcatg acctggttcc cgcattttct ctcgccgctg tccgccgcca tcgccgcggc
     4261 gattgccatt cctgcgctgc tcattctcta ttttctgaaa ctccgccggc gggagatgcc
     4321 ggtctcctca acgttcctgt ggaagaaggc ggtgcaggac cttcaggtga acgcgccgtt
     4381 ccaacgcctg cggcggaacc tgctgctgtt cctgcaactg ttgctcttgc tgatgctgct
     4441 gctggcgcta tcgcgaccgg tggcgaactt cacgccgggg gcggggaagt tgagcgtgat
     4501 cctgatcgac cgctcggcat cgatgagcgc cgctgacgaa gcggggggca agacgcgcct
     4561 ggatgaagcc aagcgccgcg cgaaggaatt ggtctcgtcg ctcgagcgca acggtcaggc
     4621 gacgatcatc gccttcgatg acacacccga gatcatgcag accttcaccg gcgatgcgca
     4681 ggcgctgcga aacgcgatcg atcgcatcgc gccgaccgat cgaaaatcgc gcttgaagat
     4741 ggcgttccaa ctggccgacg cgcaggcgtc gtacatcccc gagcagaacc gatcgaacgt
     4801 gaagccggac gtgcggctct attccgacgg ccgcgtgctg gacgccaacg aactgctgct
     4861 gcatggcgat ctgaagtacg agaagatcgg caccgactcg gccagcaaca tcgccatcgt
     4921 cgcgctggac gccaagcgga attacgaatt gcccacgcag gtgcaggtgt tcgcgcggct
     4981 ggcgaacttc ggcgtgcagc cgaagaccgc cgacgttcag ctgacgatcg acggcgccct
     5041 ccgcagcatc gccgcggtgt ccctgccgcc ggatcggtgg aacgatccga actgggtgaa
     5101 ggatcatccc ggcgaaaagg acgacacctt ccaggcgaaa gacagcgtcg agttcaccat
     5161 cgacctgcct accgccggcg tcatcaaggt cgagcagatg agcaagcaag gcgacctgct
     5221 gtccgccgac gacagcgcgc aggtcgtcgt cccgccgccc aagtcgttgt cggtcgcgct
     5281 ggtcaccacg gaaaattatt acctcgaccg cgcgatccag agcatgaacc tgaaggaccc
     5341 ggtccggctc acgccggacg aatacgaaca gaaacatccc gacaaatacg acgttatcat
     5401 cttcgatcgc tattacccga cgtggacgcc cgatgcgggc aacttcatct acgtcggctg
     5461 cgcgccgacc gccgccaacg cgaaggtgaa ggccgtcaag gagaacgagc attacgtcgt
     5521 ggaagacgac gtcggcgtgc tcgattggca gcgcgatcat ccgatcctgc gccacctgaa
     5581 cctgtcgaaa gtcttcgccg cccacgcgct gaagctcacg ctgccgccgg atgcgcagac
     5641 gctgatcgac ggcaccaagg ggccgatggt cgtgctcagc cgcgacgacc gcacgaccaa
     5701 cctgatcgtc acattcgacc tgctcgacag caattggccg ctgaagctca gcttcccgat
     5761 cttcctgcac aacgcgctgc agttcctggc gctgggctcg gacatgaacg tgcggcccag
     5821 ctacgaaccc ggcgcaacgc cgaagctgcc gcgcgcgaaa ctcgaagagg ctcgtcacgg
     5881 cctgaccagc ctcgcgctag acgggccgac gggtcgcatc agcgttccgg tgccgcccac
     5941 cggcgacttc gcgctgcccg cgctcaaccg cgtcgggcta tacacgaccg acccaccgg
//
LOCUS       DATIMH010000136         4292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2054319, whole genome shotgun sequence.
ACCESSION   DATIMH010000136 DATIMH010000000
VERSION     DATIMH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054319"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /locus_tag="VLI90_02285"
     CDS             <1..1555
                     /locus_tag="VLI90_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13059.1"
                     /translation="LAIAGRTFAAAESAAVDPTPLGDLFSSRVDGIALRPPAGGTLVR
                     QVNSDDIVRFVYPDRNWDLRLKKVHLAKPTPLSFEPTGNLGGGLLQMEADQLKASNPS
                     ADVLVQEVRDVGPTKVGFIAARYNVGVDRALAQQAIVRSDDNQTFYVLQFVTPGQAQN
                     AEGIDPQEAAAKSAFDAVLPTIKLLDRGVLKEEQDQRLFRTRELFATWDERRIKSALI
                     PDQYLRVLRDGKDAGYLHVTERMAEHGGHEGVEIGLQSHLEGDLKPAATDAPVAATAS
                     ASAGEVVPAAVPAAPSPMLPARPPRIDRFAKLFVTFDRMHEDWSIETNSDDGRTGPVR
                     TSELGNSDKEIHHKLDRQAMAADEVVDPKDKKQPAVRTTEKYTLSVSSYQKVATGAPV
                     KRELPVFYLPQALGHLLPRLLPLGEAQTYMFASYINDQREVMARYVDVGREQDVVLDG
                     QHVRAVPVLDRIGVQGAATTHYLTRDGQWLGSVNETSKVSVLPADEAEIKRLWKDAQG
                     GAGIDKKVP"
     gene            1664..2380
                     /locus_tag="VLI90_02290"
     CDS             1664..2380
                     /locus_tag="VLI90_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13060.1"
                     /translation="MVRSLAKVIFLAVLVVAGSYGIVYYRDHFSADKKIAALEQEKRQ
                     LQQVVQRLSDERRVADVLVTDQKVVNGVQQTTLLFVEYGRDGSTLPPKSFTVEGDQAH
                     IDALVIKFDHGFVEQNDPLRGCSIALFTRIYGDRQTPQQAFSIDRPGQVPDFYRGSSP
                     QVSQFEQDLWANFWRLADDPSYAREKGVRIANGQGVWGPFKPDQLYTITIEANGGLNI
                     THEPLKGIYREALKRNTAAM"
     gene            2457..3572
                     /locus_tag="VLI90_02295"
     CDS             2457..3572
                     /locus_tag="VLI90_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSV13061.1"
                     /translation="MKPSLHPPTWQQQALGFAVGMLGVLCIAGLDKITGPNIHIGVLY
                     WVPVGIAAWLGGRVPSYVLAIMAIAFWYQFSFPPADHLLPLNLKIWNVAVRVIYYPAV
                     AEMVIYVRNTERRLERLVANRTAELRLEIAERERAQAGQSKLAAQVSDAEDAERRRLA
                     YDIHDALSQMLGVIKLNLETAVAESPTDSQQYARLSDVVNVVNDLIRQTRDLTFDLHP
                     AMLEDLGLVPTLRQFADEFHRRTLAEVTVSEAGETLKLPSALASYLFRSTKELVSNSV
                     RHGNAAEILITVHWLDEGVRIVVDDDGKGFDPQAVQASTGRRGLGLAGIQERLTFLGG
                     LLRLESQPGQGTRVIMDAPAPRQQVASAAVAPVASVH"
     gene            3596..4249
                     /locus_tag="VLI90_02300"
     CDS             3596..4249
                     /locus_tag="VLI90_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSV13062.1"
                     /translation="MSIRALLVEDHTLVRSGIRALLEASKQVQVVGEAAEGRQAVDLA
                     RQLQPELVLMDIAMPELNGIEAARQIRAMMPDVRVVMLSMYADQQYVFESLKAGANGY
                     VLKGSAFQELMNAITTVMGGKNYISPALSDTVMTDYVRRAQGEQRDTELDKISGRERE
                     VLQLIAEGKSSAEVAELLHISVRTVDTHRHNIMTKIDVHSIAGLTKFAIRHGLCVLT"
BASE COUNT          819 a         1271 c         1452 g          750 t
ORIGIN      
        1 gttggcgatc gccggtcgga cgttcgccgc ggcggaatcg gccgcggtgg atccgacgcc
       61 gctgggggac ctgttctcga gccgcgtcga tggcatcgcg ctgcgcccgc ccgcgggggg
      121 aacactggtg cggcaggtga atagcgacga catcgttcgc ttcgtctacc cggatcgaaa
      181 ctgggacctt cggctgaaga aggtccacct ggccaagccc acgccgctgt cgttcgaacc
      241 caccggcaac ctgggcggcg gtctgctgca gatggaggcg gaccagctga aggcgtcgaa
      301 tccctcggcg gatgttctcg tgcaggaggt gcgcgacgtg ggcccgacga aggtcggctt
      361 catcgccgcg cgatacaacg tcggcgtgga tcgggcgctg gcgcagcagg cgatcgttcg
      421 atcggacgac aaccagacgt tttacgttct gcagttcgtg acgccggggc aggcgcagaa
      481 tgcggaggga atcgacccgc aggaggcggc ggcgaagtcg gcgttcgacg cggtgctgcc
      541 gacgatcaaa ctgctggacc gcggcgtgct gaaggaagag caggaccagc gcctctttcg
      601 cacgcgcgag ctgtttgcga cgtgggacga gcggcggatc aagtccgcgc tgatccccga
      661 tcaatacctt cgcgtgctgc gcgatggcaa ggacgcgggt tacctgcacg tgaccgagcg
      721 catggccgag catggcgggc acgaaggcgt ggagatcggg ctgcagtcgc acctggaagg
      781 cgacctgaaa cccgcggcga ctgacgcgcc ggtggcggcg actgcatcgg cgtcggcggg
      841 cgaagtggtg ccggccgccg tgccggccgc gccgtcgccg atgctgccgg cgcgtccccc
      901 gcggatcgat cgcttcgcga aattgttcgt cacgttcgat cgcatgcatg aagactggtc
      961 gatcgagacc aatagcgacg atggccggac cggcccggtg cgcacgtcgg agctgggcaa
     1021 cagcgacaag gaaattcatc acaagctgga tcgccaggcg atggcggcgg acgaagtggt
     1081 cgatccgaag gacaagaagc agcccgcggt gcgcacgacg gagaagtaca cgctgagcgt
     1141 cagctcgtat cagaaggtgg cgaccggcgc tccggtgaag cgcgagctgc cggtgtttta
     1201 tttgccgcag gcgctgggcc acctgctgcc gcggttgttg ccgctgggcg aggcgcagac
     1261 gtacatgttc gcatcgtaca tcaacgatca gcgcgaggtg atggcgcgat acgtggacgt
     1321 cgggcgcgag caggacgtgg tgctggacgg ccagcacgtg cgcgcggtgc cggtgctgga
     1381 tcgcatcggc gtgcaagggg cggcgacgac gcactatctc acgcgcgacg gccaatggct
     1441 cggcagcgtg aacgagacgt cgaaggtgtc cgtcctcccg gcggacgagg cggagatcaa
     1501 gcggctgtgg aaggacgcgc aaggcggcgc gggcatcgac aaaaaggtcc cataattcaa
     1561 cgcattcgcc gcttgcggct tagcgtgctt tcgaatttcg accaatcaag attgaaacaa
     1621 acgctaagcc gcaagcggcg tcgataacgt acaatactca tacatggttc gctcgctcgc
     1681 caaggtcatc ttcctcgccg tcctggtcgt cgccgggtcg tacggcatcg tttactaccg
     1741 cgaccatttc tcggcggaca agaagatcgc cgccctcgag caggaaaaac ggcagctgca
     1801 gcaggtggtg cagcggctga gcgatgaacg ccgggtggcc gacgtgctgg tgaccgatca
     1861 gaaagtggtg aacggcgtgc agcagaccac gctgctgttc gtggaatatg gccgcgacgg
     1921 ttccaccttg ccgccaaaat cgttcacggt cgaaggcgat caagcgcaca tcgacgcgct
     1981 ggtgatcaag ttcgatcacg gcttcgtcga gcagaacgac ccgctgcgcg gctgcagcat
     2041 cgcgctgttc acgcggatct acggcgatcg gcaaacgccg cagcaggcgt tcagcattga
     2101 tcgacccgga caggtgccgg atttttaccg gggctctagc ccgcaggtga gccagttcga
     2161 acaggacctg tgggccaact tttggcggct ggcggatgat ccttcgtatg cgcgggagaa
     2221 gggcgtgcgc atcgccaacg gccaaggtgt gtggggcccg ttcaagccgg accagctgta
     2281 cacgatcacg atcgaagcca acggcggttt gaacatcacg cacgagccgt tgaagggcat
     2341 ttatcgcgag gcgctgaagc gcaacaccgc ggccatgtag atcgatcgcg taattcgcgg
     2401 ccgcctctcg aggtagacaa gaatgtctgc cccaccagtc agactcagtt aaccttatga
     2461 aaccgtccct gcacccaccg acgtggcagc agcaggcgct tggctttgcc gtcggcatgc
     2521 tgggtgtgtt gtgcatcgcc gggcttgata aaatcaccgg gccgaacatt cacatcggcg
     2581 tgttgtactg ggtgcccgtg gggatcgccg catggctggg tgggcgggtg ccctcctacg
     2641 tgctggcgat catggcgatc gccttctggt atcaattcag tttcccgccg gccgaccacc
     2701 tgctgccgct gaaccttaag atctggaacg tcgccgtacg tgtcatctac tacccggcgg
     2761 tggcggagat ggtgatctac gttcgcaaca ccgagcgccg ccttgagcgc ctggtggcga
     2821 accgcacggc ggaattgcgc ctggagatcg ccgagcgcga acgcgcccag gcggggcagt
     2881 cgaagctggc ggcgcaagtt tccgatgccg aggatgccga gcggcgtcgg ctggcctacg
     2941 atattcacga tgccctcagc caaatgctgg gggtgattaa gctgaacctt gagaccgcgg
     3001 ttgccgaaag cccgacggac agccagcaat atgcccggtt gagcgacgtt gtaaatgtcg
     3061 tcaacgactt gatccgccag acgcgcgatc tgacgttcga cctgcacccg gccatgttgg
     3121 aagacttagg cttggtgccg acgctgcggc agtttgccga cgaatttcat cgccggacgc
     3181 tcgcagaagt gaccgtcagc gaggcgggcg agacgctgaa actgcccagc gccttggcca
     3241 gctatctgtt ccgctcgacc aaggaactgg tgagcaactc cgtccgccac ggcaacgcgg
     3301 ccgagatcct gattacggtg cattggttgg acgagggcgt acgaattgtg gtagatgatg
     3361 acggcaaagg gttcgaccca caggccgtgc aggccagcac tggccgccgc gggctgggtc
     3421 tggcgggcat tcaggagcgg ttgacctttt tgggtggatt gctccggctg gaatcgcaac
     3481 cggggcaggg cacgcgggtg atcatggatg ccccggcacc gcggcaacag gtggctagcg
     3541 ccgcggtagc tccagtagca tcagtgcatt agcgtcttga accggatcga caaacatgtc
     3601 cattcgcgca ttgctggttg aagatcacac cttggtgcgt tcgggcattc gcgcgctgct
     3661 ggaagcgtcg aagcaggtgc aagtggttgg cgaggccgcc gagggccggc aggctgtcga
     3721 tcttgcccga cagctgcaac ctgagctggt gctgatggat atcgccatgc cggagctgaa
     3781 cggcatcgag gccgcccgtc agatccgggc gatgatgccg gatgtgcggg ttgtcatgct
     3841 gtcgatgtac gccgaccagc agtacgtttt cgaatccctg aaggcggggg cgaacggtta
     3901 tgtcctgaag ggatcagcct ttcaggagct gatgaacgcg atcacgacgg tgatgggcgg
     3961 gaagaactac atcagcccgg cgttgtccga caccgtcatg accgattacg tccgccgggc
     4021 gcagggcgag cagcgcgata cggagctgga caagatctcc ggccgcgagc gcgaagtgct
     4081 gcagttgatc gccgagggca agagcagcgc cgaagtggcc gaactgctgc acatcagcgt
     4141 ccgcaccgtc gatacccatc gacataacat catgaccaag atcgacgttc actccatcgc
     4201 cggattgacg aaatttgcca ttcgtcatgg cctctgcgtg ctcacctgag cctctcggct
     4261 cagccccaat accccatctt gctgcctcgc ga
//
LOCUS       DATIMH010000137         1637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3499143, whole genome shotgun sequence.
ACCESSION   DATIMH010000137 DATIMH010000000
VERSION     DATIMH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3499143"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..>1637)
                     /locus_tag="VLI90_02305"
     CDS             complement(55..>1637)
                     /locus_tag="VLI90_02305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV13063.1"
                     /translation="HDSPAVESIRASYEETQYPGGAFTASHPDHLRVIAALGGFTATT
                     SAANCRVLEIGCAIGRNLMPMAQSMPNSRFLGIDLSPRQIEIGQAVARQLGINNLELR
                     CQDLLDFPADEGPFDYIIAHGVYSWVPPHVRERLLQICGRSLSPGGLAYISYNTLPGW
                     HLLGALRDMMLFHSQGAKNGTDRAALSREMVKFASQPWPLWPAYRQLMNDEQKQMADR
                     SDWYLLHEFVAPVNHPFHFLDFARDAAGHGLHHLGDALTTIDKSYLLTPEMQQQLRQF
                     AEDDELKRQQYLDYLGGRAFRASVLCRADAPHTPARPAMALQQMYIAGRLTEEPAQDE
                     ATKTPIVRFSAADGGEFKLNLSDMGMIAAMGRISGAWPHAVQFAELLDLATMLSPPDP
                     QQHDPALDLARMLLLAYRAGVVELWTGPTDFIATNLQRPHATALARHQVAMNEDLINL
                     RHQYLRITPTIAAMVPLMDGARDRAALLRELTAMIDRRTLSLPGVDESAVATDSLLDT
                     ILTEFHLKSLLTGPLAQR"
BASE COUNT          279 a          533 c          530 g          295 t
ORIGIN      
        1 agtatccttc gacttcgctc aggacaggcc tccgaacccg gcgtgggggg ctcgtcagcg
       61 ctgcgccaat ggccccgtca gcaggctctt taggtggaac tcggtcagga tggtgtcgag
      121 caggctatcg gtcgcgacgg ccgattcatc gacgccgggg aggctcagcg ttcggcggtc
      181 gatcatcgcc gtcaactcgc ggagcagagc cgctcgatct cgtgcgccat ccatcagcgg
      241 caccatcgcg gcgatcgtcg gcgtgatgcg aaggtactgg tgccgcaggt tgatcaagtc
      301 ttcgttcatg gccacctggt ggcgcgccag cgccgtcgcg tgcgggcgct gcaagttcgt
      361 tgcgatgaag tcggtgggac cagtccacag ctcgaccacg ccggcgcgat atgccagcag
      421 cagcatgcgg gccaagtcca gcgccggatc atgttgctgt ggatccggcg ggctcaacat
      481 cgtcgccagg tcgagcaact ccgcgaactg caccgcgtgc ggccaggcgc cgctgatccg
      541 gcccatcgcc gcgatcatgc ccatgtcgct caggttcaat ttgaactcac caccgtcggc
      601 ggcgctgaag cgcacgatgg gcgtcttggt cgcctcgtcc tgcgcgggtt cttccgtcaa
      661 gcggccggcg atgtacatct gctgcagcgc cattgctggc cgcgctggtg tgtgcggcgc
      721 atcggccctg cacagcaccg acgcgcggaa cgcacggccg cccaggtaat ccaggtactg
      781 ctgccgcttc agctcatcat cctccgcgaa ttgccgcaac tgctgctgca tctccggcgt
      841 caggaggtac gacttgtcga tcgtcgtcag cgcatcgccc aggtgatgca gcccgtgtcc
      901 cgcggcgtcg cgcgcgaaat cgaggaagtg aaacggatga ttcaccgggg cgacgaactc
      961 gtgtagcaga taccaatcac tacgatcggc catctgtttc tgctcgtcgt tcatcaattg
     1021 ccgatacgcc ggccagagcg gccagggttg ggaggcgaac ttcaccatct cgcgcgagag
     1081 cgcggcgcga tcggtgccgt ttttcgcgcc ctggctgtgg aacaacatca tgtcccgcag
     1141 cgcgcccagc aggtgccagc ccggcagcgt gttgtagctg atgtacgcca acccgccggg
     1201 cgataagctg cggccgcaga tctgcagcaa tcgctcgcgc acgtgcggcg gcacccacga
     1261 atagacaccg tgcgcgataa tgtaatcgaa cggcccctcg tcagcgggga agtccagcag
     1321 gtcctggcag cgcagctcca ggttgttgat ccccagctgg cgcgccaccg cttggccgat
     1381 ctcgatttgc cgcggcgaaa ggtcgatccc gaggaaccgg ctgttcggca tcgactgcgc
     1441 catcggcatc aaattgcggc cgatcgcgca gccgatctcc agcacgcggc aattcgctgc
     1501 agacgtcgtt gcggtgaatc cgccaagggc ggcgatcacg cgcaagtgat cgggatgtga
     1561 cgcggtgaac gcaccgcccg ggtattgcgt ttcctcgtac gaggcgcgga tcgattcaac
     1621 ggcgggacta tcgtgcg
//
LOCUS       DATIMH010000138         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_613067, whole genome shotgun sequence.
ACCESSION   DATIMH010000138 DATIMH010000000
VERSION     DATIMH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613067"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VLI90_02310"
     CDS             complement(<1..1376)
                     /locus_tag="VLI90_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSV13064.1"
                     /translation="MSDRSRNDCDRVRCIGAASRNDLWCNQLGETMTRLLSRATLAAL
                     LFAGTASVAYAASDYASVTDSRLANPEPQNWLMTRGNYQGWSYSSLDQINTSNVKNLV
                     PVWSFSTGMDSGHEAPPIVNNGVMFVATPHNQVLALNAATGDLIWRYKRPEPEGFSAL
                     HNTSRGVALYGDKVFFPALDAVLVALDAKTGKVVWETKVEDWKTGYYMTVAPLAEKGK
                     VLVGVSGGEFGVRGFIQAFDADTGKSVWKTYSVPEPGQPGSDTWQKADTWKTGGAPTW
                     MNGNYDPKTNTVYWGTGNGSPWFGDQRPGDNLYTSSTLALDGDTGAMKGYFQYHQNDS
                     WDWDEMNAPLLVDFQKDGATISGLLKPARNGYLYWLSRGADGKIKFINAEAYVKQNVF
                     KSIDPTTGRPVVDMDHKPGTGKEASFCPSLWGGKDWPFEAYNPKTGMIYIPSNDNHCG
                     HMKGAVEER"
     gene            complement(1354..1929)
                     /locus_tag="VLI90_02315"
     CDS             complement(1354..1929)
                     /locus_tag="VLI90_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HSV13065.1"
                     /translation="MGTPKLSRRTTLSGCLLTVAAGTTSAAWAEGETDPAREMPPQPG
                     DPFAFLTGDKKGQVIKANDLPLGGPQVQAYPIDPKSNVVRDGSRLNLIVLIRLDPSSL
                     SEETRLRAADGVVAYSAVCTHQGCPVNMWSSQHNAFFCSCHGSIYDPRNAARVLAGPA
                     PRPLPSLGLKLENDVPVVAAGFTGHVGPIAK"
     gene            2208..>2936
                     /locus_tag="VLI90_02320"
     CDS             2208..>2936
                     /locus_tag="VLI90_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSV13066.1"
                     /translation="MQRTIRTPTLEIAFLDTGPPDGPPVILLHGFPDDPHCYDDVAPP
                     LADAGHRVLVPWLRGYGPTRFLHATTPRSGQQAALGGDLRDFMDALGIGRAILAGYDW
                     GGRAACIVAALWPERVTGLLSITGYNIQAIGTANRPAAAAQEYRYWYQWYFHTQRGRA
                     GLEHNRRDICRLLWQLWSPNYRFDDAAYDLTAASFDNPDFVDVVIQSYRHRYGNAPGD
                     PALEAIEARLATRPLITVPTINLHG"
BASE COUNT          520 a          927 c          880 g          609 t
ORIGIN      
        1 cgctcctcaa ccgcgccctt catgtgcccg cagtgattgt cgttcgacgg gatgtaaatc
       61 atccccgtct tggggttata cgcttcgaag ggccagtcct tgccgcccca gaggctcggg
      121 cagaagcttg cctccttacc cgtacctggc ttgtggtcca tgtcaacaac gggtcgtccg
      181 gtggtggggt caatgctctt gaagacgttc tgcttcacgt aggcttcggc gttgatgaat
      241 ttgatcttgc cgtcggcgcc ccgcgacagc caatagagat aaccgttgcg cgcaggcttg
      301 agcaggccgc tgatggtggc accatccttc tggaaatcga ccagcagcgg tgcgttcatc
      361 tcgtcccagt cccaggagtc gttctggtgg tactggaagt aacccttcat cgcgccggta
      421 tcgccgtcga gcgcgagggt ggacgaggtg tagagattgt cgccgggccg ctgatcgccg
      481 aaccatggcg agccattgcc ggttccccaa tagaccgtgt tggtctttgg atcatagttg
      541 ccgttcatcc aagtcggcgc accgccggtc ttccaggtgt cggccttctg ccaggtgtca
      601 ctacctggct gccccggctc gggcacactg tatgtcttcc acaccgattt gccggtgtcg
      661 gcgtcgaagg cctggatgaa accacgcacc ccgaactcgc cgccggagac gccgacgagc
      721 accttgccct tctccgccag cggagcgacg gtcatgtaat aaccggtttt ccagtcctcg
      781 actttggttt cccagacgac ctttccggtt ttggcatcaa gtgcgacgag gacggcatcc
      841 agcgccggga aaaacacttt gtcgccatac aatgcaacgc cgcggctggt gttgtgcaac
      901 gcgctgaagc cctccggctc cggccgcttg tagcgccaga tcagatcacc ggtggcagca
      961 ttgagcgcca gcacctggtt gtgcggcgtg gcgacgaaca tcacgccatt gttcacgatc
     1021 ggcggcgctt cgtgaccgga atccataccc gtcgagaagc tccacaccgg cacgaggttc
     1081 ttcacgttgc tggtgttgat ctggtcgagc gaactgtaac tccagccctg gtagttgccg
     1141 cgtgtcatga gccagttctg cggctcggga ttcgcgaggc gcgaatcggt caccgacgcg
     1201 tagtcggatg ccgcgtaggc cacgctagcc gttccggcga agagcagggc cgccaaggtt
     1261 gcgcggctca ggagtctcgt cattgtttcc cccaattggt tacaccacaa atcattccgg
     1321 ctggctgcac cgatgcagcg aactcggtca cagtcatttc gcgatcggtc cgacatgacc
     1381 ggtgaagcct gcggcgacga ccggcacatc gttttcaagt ttcaggccca ggctgggcaa
     1441 cggtcgcggc gccgggcccg ccagcacgcg tgcagcgttc cgcggatcgt agatcgagcc
     1501 gtggcatgaa cagaagaacg cgttgtgctg gctgctccac atgttcaccg ggcagccctg
     1561 atgggtgcaa acagccgagt aagcgacgac tccgtctgcc gcccgcaagc gcgtttcctc
     1621 gctgagcgat gatgggtcca ggcgaatgag gacgatcaga ttcagtcgtg agccatcccg
     1681 cacgacgtta ctctttggat cgatggggta ggcctgcacc tgtgggccgc cgagcggaag
     1741 gtcattggcc ttaatgacct gacccttctt gtcgcccgta aggaaagcaa acgggtcgcc
     1801 gggttgcggg ggcatctccc tggccgggtc ggtctcgccc tctgcccatg ctgctgatgt
     1861 cgtgccggca gcaaccgtca gcagacagcc acttaacgta gtacggcgcg acaatttggg
     1921 cgtgcccatt cccgtcgccc ccgttcgatg tccaggcggc acgctagttt tcgcccgcaa
     1981 cgggtgtcaa ggatgcacgt caatctgctt tactacgtgg aaacaccaac atcgctcggt
     2041 tggagggcac ttcggtctcg attttgtctg tatcgtgacg actcgtagcg gtatgccgcg
     2101 acaagtctca cattttaata ccaccttcgg gccagacccg cgatcgcccg gaatcgtgtc
     2161 acgcattcgt gaatacgatt tgggcccgcg gagtttgaaa tgctcccatg caacgcacca
     2221 tacgcacacc gacgctggag atcgcgtttc tggacaccgg gccgcccgat ggtcctccgg
     2281 tgattctgct gcatggattt cccgacgacc cgcattgcta cgacgatgtc gcgccgccat
     2341 tggccgacgc gggacatcgc gtattggtgc cgtggctgcg cggctacggc ccaactcggt
     2401 ttctgcacgc aaccacaccg cgttccggtc agcaggcagc gttgggcggc gatctgcgcg
     2461 acttcatgga cgcgctcggc atcggacgcg ccatacttgc agggtatgat tggggcgggc
     2521 gcgcggcgtg catcgtcgcg gcgttgtggc cggagcgtgt gactggattg ctgtccatca
     2581 ccggctacaa catccaggcg atcggcactg ccaaccgccc cgccgcagca gcgcaggagt
     2641 accgatattg gtatcaatgg tatttccaca cgcaacgcgg ccgtgccgga ctggagcata
     2701 accgccggga tatctgccgc ctgctgtggc aactctggtc gcccaactac cgcttcgatg
     2761 atgctgccta cgacctcacc gccgccagtt tcgacaatcc ggactttgtc gatgtggtga
     2821 tccagtccta ccgtcaccgc tacggcaacg cacctggtga tcctgcgctg gaagctatcg
     2881 aagcgcggct cgccacacgg ccgctgatca cggtgcccac catcaatctg cacgga
//
LOCUS       DATIMH010000139         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_900567, whole genome shotgun sequence.
ACCESSION   DATIMH010000139 DATIMH010000000
VERSION     DATIMH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900567"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..542)
                     /locus_tag="VLI90_02325"
     CDS             complement(198..542)
                     /locus_tag="VLI90_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV13067.1"
                     /translation="MTAASERTTRSADADRKSKKTVLVVDDEGDLLDLITYNLQRNGF
                     AALTATSGDAALEIASRELPNLTLLDLMLPGMNGTEIARRLKADSRTATIPIIMPTAK
                     SEETDVIAGRRS"
     gene            684..1964
                     /locus_tag="VLI90_02330"
     CDS             684..1964
                     /locus_tag="VLI90_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13068.1"
                     /translation="MPRSALGFMHLVGLLIGMLAIGGCEPISHISWSPDGARAAYFVP
                     VQGKLVPGVSYVLDADGKKMASLGPTFGSFAWSPDSKSIYFGAYDNQQPATVPIERKW
                     LVDAEDQQPPEAPANDEFPPMVLTRWSDGKAERLAPVGNRYVVFVQLSPDQNWVAVMT
                     SAKETGTDGRPQLFVYNVASKKLYAVSDYCGNAMCFTGPDRLAYVETMRDHHQPLQTG
                     QIIEVKLDESSEQLERTPLVDLIPSTTTFMQPTGDGLLFTTVAASVPVKASKENEHRP
                     PGLFHYTRANGGVTAMAEATEQLFMPSPDGKRILYIKLTPASEKMPLKRELCVMNANG
                     SDEHVLRDLTIFGQQPPMWPGWRGNDEITFVGSKAEDQPVQEGKDPRIAFDVVQYKIN
                     AQGALEPIKTLSDGWPIEMKPSMKKSDYTAPVSN"
     gene            2039..2584
                     /locus_tag="VLI90_02335"
     CDS             2039..2584
                     /locus_tag="VLI90_02335"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSV13069.1"
                     /translation="MKIYTKTGDDGSTGLIGGERVAKSDPRIDCYGTVDELNAAIGLA
                     AVVADASLVELLRAVQNDLFVIGSHLATPSHVPQNVSLPVLDDAMIHRLEMQIDAADA
                     ALPPLRNFILPGGTECAARLHLARTICRRAERLLVNFAGDRPVPGVIITYLNRLSDWL
                     FVQARSANATAGVADIPWQKP"
     gene            2960..>3413
                     /locus_tag="VLI90_02340"
     CDS             2960..>3413
                     /locus_tag="VLI90_02340"
                     /inference="COORDINATES: protein motif:HMM:NF016553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13070.1"
                     /translation="MSTTLLQFANVNDSAAATTKKLIKQAALAEYFRTLDDDDLRLAV
                     RYAAGRPFPATSERTLNIGGAIVSEVVLGILKLDPRTYHDLVVTSGEIGEALARAWPP
                     DNAPAQSPAAPPRGTHAHAPLTLGDLSEAFEDLSRTGNVERKRELLHAL"
BASE COUNT          648 a         1030 c         1120 g          615 t
ORIGIN      
        1 gcgattgatc gcacgtgcgt cgagcacaag gttagcagtc gaaggatcta gccgagaagt
       61 aagtgaatct tcatttgtgg aatggccgcc gcttgcggct tagcgtcttt ttcttcggaa
      121 gaaacacatg ctaagccgca agcggctgtg cgcgctcttc gcgaacgagt aggtcaccga
      181 ctcctcggct cgatccttca actccggcgg ccggcgatca cgtcggtctc ttcgctcttg
      241 gcggtcggca tgatgatggg gatcgtcgcg gttcggctgt cggccttcag ccggcgcgcg
      301 atctccgtgc cgttcatgcc cggcagcatc aggtcgagca gggtcaggtt cggcagttcg
      361 cgcgacgcaa tctccagcgc tgcgtcccca ctcgtcgccg tcagcgcggc gaaaccgttg
      421 cgctgcaggt tgtaggtgat caggtcgagc aagtcgccct cgtcatcgac gacgaggacc
      481 gtcttcttgg acttgcgatc ggcgtcggcc gaccgggtcg tgcgctcgct ggcggcagtc
      541 atcggctgtc tcccataatc cggcccgcga tgccggaacc cgcgtccatg ctatactcgc
      601 attcatgcgg accatcgatc cgcggggcag ggcaacgggt caagcgatca gcgcggtctt
      661 aacgaacgac gaaggagacg caaatgccac ggtcggcttt ggggttcatg cacttggtcg
      721 gtttactgat cggcatgctg gcgatcggtg gttgtgaacc catttcacat atttcatggt
      781 cgcccgacgg cgcccgggcg gcgtatttcg tgccggtcca gggcaagctc gtgccgggcg
      841 tgtcttatgt gctggacgcg gacggaaaga agatggccag cttggggccg accttcggca
      901 gcttcgcgtg gtcaccggat tcgaagtcaa tttacttcgg cgcgtacgac aaccagcagc
      961 cggcgacggt gccgatcgag cgtaagtggc tggtggatgc cgaggaccag caaccgcccg
     1021 aagcgcccgc gaacgacgaa tttccaccga tggtgctgac gcggtggagc gatggaaagg
     1081 ccgaacggct ggcgccagtg gggaatcgct acgtcgtctt cgtgcaactg tcgccggatc
     1141 agaactgggt ggcggtgatg acgtcggcga aggagacggg gacggacggg cggccgcagt
     1201 tgttcgtgta caacgtcgcg tcgaaaaagc tgtacgccgt cagcgactat tgcggcaacg
     1261 cgatgtgttt cacggggccg gatcggttgg cgtacgtcga gacgatgcgc gaccatcacc
     1321 agccgctgca gacggggcag atcattgaag tgaagctgga cgaatcgagc gagcaactgg
     1381 agcgcacgcc gctggtagat ctcattccga gcacgacgac gtttatgcag ccgaccggag
     1441 acgggctgct gttcacgacg gtagcggcgt cggtgccggt gaaggcctcg aaggagaacg
     1501 agcatcgtcc gccggggttg ttccactaca cgcgcgccaa cggcggcgtg accgcgatgg
     1561 ccgaagcgac cgagcagttg ttcatgccca gccccgacgg caagcgaatc ctgtacatca
     1621 agctgacgcc ggccagcgag aagatgccgc tcaagcgtga gctgtgcgtg atgaacgcca
     1681 acggcagcga cgagcacgtg ctgcgcgatc tcacgatctt cggacagcaa ccgccgatgt
     1741 ggccggggtg gcgtgggaat gacgagatca cgttcgtcgg ttcgaaagcg gaagaccagc
     1801 cggtgcagga aggcaaggac ccgcggatcg cgtttgacgt ggtgcagtac aagataaacg
     1861 cgcagggcgc gcttgagccg attaaaacgc tgagcgacgg ctggccgatc gagatgaagc
     1921 cgtcgatgaa gaaatcagat tacacggcgc ccgtgtcgaa ctagtgcggg accgccgctt
     1981 gcggcttcgc aaactacatc gtctgattca cgaagccgca agcggctgcc tttgcaagat
     2041 gaaaatctat accaaaaccg gcgacgacgg cagcacggga ttgatcggcg gcgaacgcgt
     2101 cgccaagtca gacccgcgga tcgactgcta cggcacggtg gacgagttga acgccgccat
     2161 cggcctcgcg gcggtggtcg ccgatgcgtc gctcgtcgag ctgctgcgcg cggtgcagaa
     2221 cgacctgttc gttatcggct cgcacctggc gacgccgagc cacgtgccgc agaacgtgtc
     2281 gctgccggtg ctggacgacg cgatgattca ccgcctggag atgcagatcg acgccgctga
     2341 tgcggcgttg ccgccgctgc ggaatttcat cctgccgggg ggcaccgagt gtgccgcgcg
     2401 attgcatctg gccagaacga tctgccggcg tgccgagcgg ctgctggtta actttgctgg
     2461 cgatcgtccg gtgccgggcg tgatcatcac ctatctcaat cgcctgagcg attggctgtt
     2521 cgtgcaggcc cgcagcgcaa acgccaccgc cggtgttgcg gatatcccgt ggcagaagcc
     2581 gtgacgcggc gaaaataaat gcgaccgcga agacgcgaaa gcgcgaagcc aagacgcgaa
     2641 gacaagtagc acgggcgtct cgcccgtgtc gagcggtgcc agacacgggc gagacgcccg
     2701 tgctactcgc gctacaaaag catcggatta atggagtggt tgatgttcgg aagccgctcg
     2761 cacgggctgg aagcccgtgt cacgttaagc gtccattccc tcatcgtgac acaggcctcc
     2821 ggcccctgcg agcgatgcag tagttcaaca atctctccct cccaatatct ctatcttccc
     2881 cttcgcgtct gtcttggcgc ctttgcgtct tcgcgctcgc gttccctggg caatctggcg
     2941 cgcgctacaa tcggatgcgg tgtccacaac gcttcttcaa ttcgcgaacg tcaacgattc
     3001 agccgcggcg acgacgaaga aactgatcaa gcaggcggcg ctggcggagt attttcgaac
     3061 gctggacgat gacgatcttc gcctcgccgt gcgttatgcc gcggggcggc cgtttccggc
     3121 gaccagcgag cgcacgctca atatcggcgg ggcgatcgtc agcgaagtcg tgctgggcat
     3181 cctaaagctc gacccgcgga cgtatcacga tctggtcgtc accagcggcg agatcggtga
     3241 agccctcgcc cgcgcatggc cccccgacaa cgcgccagca cagagcccag cggctccgcc
     3301 gcggggcacc catgcacatg cgccgctcac ccttggagat ctgtctgagg cgttcgaaga
     3361 tctgtcgcgc accggcaacg tcgagcgcaa gcgcgagctg ctgcacgcgc tgt
//
LOCUS       DATIMH010000140         4562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2633603, whole genome shotgun sequence.
ACCESSION   DATIMH010000140 DATIMH010000000
VERSION     DATIMH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2633603"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            562..807
                     /locus_tag="VLI90_02345"
     CDS             562..807
                     /locus_tag="VLI90_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13071.1"
                     /translation="MRLEIALPLQHVLHIPTVGSLLRAWRSPKNQKSIEQLFDSPQQA
                     RHAVAICAAWLGVETAPTNSVGPAWWHGDEHAPSFDV"
     gene            complement(880..1305)
                     /locus_tag="VLI90_02350"
     CDS             complement(880..1305)
                     /locus_tag="VLI90_02350"
                     /inference="COORDINATES: protein motif:HMM:NF016245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HSV13072.1"
                     /translation="MTYLPTIVLLLISNTFMTFAWYGHLKFEQVPLWKVILVSWGIAF
                     LEYVFQVPANRIGNMRGLSGGQLKIIQEVITLIVFVVFARMYLGERLTWNYYVAFGFI
                     LAAVFFVFNFRPAQPAQTVGKAPALTQISSVAVESPQTS"
     gene            complement(1477..2037)
                     /locus_tag="VLI90_02355"
     CDS             complement(1477..2037)
                     /locus_tag="VLI90_02355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV13073.1"
                     /translation="MTDALRRLIDELDQLGKTRDDAWQISREEGELLRQIALASNARV
                     IIEVGTSYGFSGLFWGAALMHTGGLLHTIDRNPAKFESSKKTFVDAGLGAHVVNLCGD
                     ATAILQTLKGPFDLAFIDADKPSTRNYFDLIWPKLRVGGSVITDNATTHREQLGEFVG
                     YVRALPNAVSAEVAVGNGIEWTIKVK"
     gene            complement(2204..2974)
                     /locus_tag="VLI90_02360"
     CDS             complement(2204..2974)
                     /locus_tag="VLI90_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSV13074.1"
                     /translation="MLRFPVVGEFAPGQVAIVRAPSTRRIVPEVEAIIDAAWSAALAR
                     PGVHLFDGPMCRLESFRVSPQRLELSLSDTSYKPFFGTHLSHPELFNRFGPEVMSNSV
                     GVSPALLSSDGFLMLGRRNQSVAYYPGRVHPFSGSLEPREQDVFGAVRRELREELSLA
                     DADIAQMVCTGIAEDLCLRHPELMFAVTATRTREQIERQLDPVEHHDVWSTPGGEAGV
                     AAALGQRDQLTPIAIASLLLWGRLSFGDAWFAQRSVEC"
     gene            complement(3011..>4562)
                     /locus_tag="VLI90_02365"
     CDS             complement(3011..>4562)
                     /locus_tag="VLI90_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSV13075.1"
                     /translation="RSGLITAGLALVYVIVMYFIVRPFLRRLQLVYERQQGLSQTMVG
                     IMLLMVLVSAYITEVIGIHALFGAFLLGAMMPKGSRFVRTITEKLEDFTVVFLLPIFF
                     AYTGLKTRIGILDNGEMWFYCSLIIFVACLGKFGGSTLAARACGIGWRESGAIGILMN
                     TRGLMELVILNVGRELGVITDAVFAMMVIMALVTTALTTPILQLVYPQKRMEEEQIVD
                     TGGGYSVLIPVSFPASGPPLVQLANAITGSDNQDRHIFALYLKRPVDRDAYRFGIDQE
                     EAPEKIEALQPLLSEAKAHKIPVTPVAFTSRDVASDIARTARLRNVDLILMGFHNPVY
                     TSSILGGTVHRVMTGSDNDVAIYVDRGVAAPRNILIPYLGSKHDRLAMELAGRMARNA
                     QVSVTVLHVVQPGRAEGESAINAKAATDRVFNDPTQPMPVTMRTMKGDSPVDVVVEQA
                     KEFDLVIIGVAEEWGLESQLLGFRAERIAQDVACSLLIVRKHGRFRAARGVPTEKPAE
                     QQRVGVDV"
BASE COUNT          853 a         1503 c         1370 g          836 t
ORIGIN      
        1 ggttggtggg gcagagattc gtgtctgcct cgcggcgcag ccgcgaattg acatatgatt
       61 ggtttacgtg ccttcggcac gaggcaggca ggaatgcctg ccccaccaaa agttatggca
      121 tcgcccagca ttggcatccc cttcgtctcg acgacccggc aattctcgac gtccagccca
      181 tttcccctca ccgttttccg cgcggcattc cgattgattt cacgaggcga cttcttagac
      241 taataatcac gattgcaagc gggcctcggc gggcgtgggc tatggccgcc gaggtggcat
      301 ggatgccgac cgggtgcgat cgtaatgtct gctggtgcat gggcttgggt ggtgccgcac
      361 acggctttgc cttcggcaat atgccttcgc ggctccttcc ccttcgactt tgccaacgtc
      421 tgatcggccg atcgatcgcc gcgcggcgtc aatagccttt gccgcgcgcc tcgaccgccg
      481 cggtccgctt cgttgcaaac gtctggagta gtcgttcaca tgtctcgtaa tcccattctg
      541 gatcttccgt taagttctat catgcggctg gaaatcgcgc tgccgctgca gcacgtgctg
      601 cacattccca ccgtcggcag tctgctgcgg gcgtggcgga gtccgaagaa tcagaagagc
      661 atcgagcagc tgttcgactc gccgcaacag gcgaggcacg ccgtcgccat ctgcgccgcg
      721 tggctggggg tggaaacggc gccgaccaac agcgtcggcc cggcgtggtg gcatggggat
      781 gaacatgccc catcgttcga cgtctgatct tcaggagagc cgggccgtcc tcgggcccgg
      841 accggcccgg ggatgggccg gctccccttg gatatcgcgc tagctcgttt gcggtgactc
      901 taccgcaacg ctgctgatct gcgtcagcgc cggcgccttc cccacggtct gggcaggctg
      961 ggccggccgg aagttaaaca cgaaaaacac cgcggcgaga ataaagccga acgccacgta
     1021 gtaattccaa gtcagccgct cgcccagata catccgcgcg aacaccacga acacgatcag
     1081 cgtgatcact tcctgaatga tcttcagttg cccgcccgac agcccgcgca tattcccgat
     1141 gcggttcgcg ggcacttgaa agacgtattc caaaaacgcg attccccatg agacgaggat
     1201 caccttccac agcggcacct gctcgaactt caggtggcca taccacgcga aggtcatgaa
     1261 ggtgttggag atcagcaaca ggacgatcgt ggggagataa gtcatgaggg tcggggtgag
     1321 ggtgatttcg aagagctaat gaccaatgct caaccttcaa atcccaatga aattctaatg
     1381 accaacgccc agcccgcgcg actgttgagg cttgggcctt gtggcttcat tgggatttgg
     1441 cttttggaca ttggcattct ggctcggcgc aagcgcctac ttcaccttga tcgtccattc
     1501 gatgccattc ccaacggcca cttccgcgct caccgcgttc ggcagcgctc ggacatagcc
     1561 gacgaactcc cccagctgct cgcggtgcgt cgtcgcgttg tcggtgatga ccgatccgcc
     1621 gacgcgcagc ttcggccaga tcaggtcgaa gtaattccgc gtcgaaggtt tatcggcatc
     1681 gataaaggcc aggtcgaacg ggcccttcag cgtctgcaag atcgcggtgg cgtcgccgca
     1741 gaggttgacg acgtgcgccc cgagcccggc gtcgacgaac gtcttcttgg acgattcaaa
     1801 tttcgccggg ttgcggtcga tcgtgtgcag caagccgccg gtatgcatca gggccgcgcc
     1861 ccagaacagc ccgctgaaac cgtagctggt gccgacttcg atgatcacgc gggcgttgct
     1921 ggccagcgcg atctgccgaa gcagttcgcc ttcctcgcgc gagatctgcc acgcgtcgtc
     1981 gcgcgttttc cccagttggt ccagctcatc gatcagtcgg cgtaaggcgt cggtcatgac
     2041 tgtaccgtag cgccgccgcg atcaattcgc aaccggcggt ggggggatga agttcccatc
     2101 tttaatagcc gcaggcgaca gccagcgggg attctcgaaa tcgcagaccg gtttagttct
     2161 cgcgggcccc cgctggtttt cgcctgcggc tatcagcggg gcgtcagcat tccacggagc
     2221 gctgcgcgaa ccacgcgtct ccgaaggaca agcggcccca tagcagcagg ctcgcgatgg
     2281 cgatcggggt cagctgatcg cgctgcccaa gggccgccgc aacgccggct tcgccgcccg
     2341 gcgtcgacca gacatcatga tgctcgaccg gatcgagctg ccgctcgatc tgctcgcgcg
     2401 tgcgcgtcgc tgtgaccgcg aacatcagct ccggatgccg caggcacaaa tcttcggcga
     2461 tgccggtgca caccatctgc gcgatatcgg catcggccaa cgacaactcc tcgcgcaatt
     2521 cccgccgtac ggcaccgaag acatcctgct cgcgcggctc cagtgagccc gagaacggat
     2581 gcacccgtcc ggggtaatac gccaccgact gattgcggcg accgagcatc aggaacccat
     2641 cgctgctcaa cagcgcggga ctgacgccga cggaattcga catgacctcg gggccgaagc
     2701 gattgaacaa ttcagggtga ctaaggtgtg tgccgaagaa cggtttgtag ctggtgtccg
     2761 acagcgaaag ctcaagccgc tgcggcgaga cgcggaacga ttccagccgg cacatcggcc
     2821 cgtcgaacaa atgcacgccc ggccgcgcca gtgcagcaga ccaagcggcg tcgatgatcg
     2881 cctccacctc cggcacgatc cggcgggtgc tcggcgcgcg cacgatcgcg acctgccccg
     2941 gcgcaaactc acctacaact ggaaatcgca acatcaccat gaggccggcg atggcatcgc
     3001 cgggcgcgac tcacacgtcc acgcccacgc gctgctgctc cgccggtttt tccgtcggca
     3061 caccgcgggc cgcgcggaag cgcccgtgct tgcggacgat cagcagcgag cacgccacgt
     3121 cctgcgcgat gcgctcggcg cggaagccca gcagctgcga ttccaacccc cactcctccg
     3181 ccacgccgat gatcaccagg tcgaactcct tcgcctgctc gaccaccacg tccaccggtg
     3241 aatcgccttt catcgtccgc attgtcaccg gcatcggctg ggttggatcg ttgaacactc
     3301 gatccgtcgc ggccttggcg ttgatcgccg actcgccctc ggctcgcccg ggctgaacca
     3361 cgtgcagcac cgtcacgctc acctgcgcgt tgcgcgccat tcgcccggcc aattccatcg
     3421 caaggcgatc gtgcttgctg cccaggtacg ggatcaaaat gttccgcggc gcagccaccc
     3481 cgcgatcgac gtagatcgcg acatcgttat cgctgccggt catcacgcgg tgcaccgtgc
     3541 cgccgaggat cgacgacgtg tagaccggat tgtgaaagcc catcaggatt agatcgacgt
     3601 tgcgcaggcg agcggtgcgg gcgatgtcgc tggcgacatc ccggctggtg aacgccaccg
     3661 gcgtcaccgg aatcttgtgc gccttcgcct cgctcagcag cggctgcagc gcttcgatct
     3721 tttccggcgc ttcttcctga tcgatgccga accgatacgc gtcgcgatcg accgggcgct
     3781 tgagatacag tgcgaagatg tgccggtctt ggttgtcgct gccggtgatg gcgttggcca
     3841 gctgcaccag cggcggcccg ctggccggga acgacacggg gatcagcacc gaatatcccc
     3901 cgccggtgtc gacgatctgc tcttcctcca tccgcttttg cggatagacc aattgcagaa
     3961 tgggcgtcgt cagcgcggtg gtcacgaggg ccatgatgac catcatcgcg aagaccgcat
     4021 cggtgatgac gccgagttcc cggccgacgt tgaggatgac cagctccatc aacccgcggg
     4081 tgttcatcag gatgccgatc gcgccgctct cgcgccaacc gatgccgcag gcgcgcgccg
     4141 ccaacgtcga tccaccgaat ttcccgaggc acgcgacgaa gatgatcagg ctgcagtaga
     4201 accacatctc gccgttgtcg aggatgccga tgcgcgtctt caacccggtg taggcgaaga
     4261 agatcggcag caaaaacacg acggtgaaat cttccagctt ctcggtgatc gtccgcacga
     4321 accggctgcc cttgggcatc atcgccccga gcagaaacgc gccgaagagc gcgtgaatgc
     4381 cgattacttc cgtgatgtac gccgacacca gcaccatcag cagcatgatg ccgaccatcg
     4441 tctggctcag cccctgctgc cgttcgtaca ccagctgcag ccggcgcaag aacgggcgga
     4501 cgatgaaata catcacgatg acgtaaacca gcgccaaccc cgcggtgatc aggcccgagc
     4561 gc
//
LOCUS       DATIMH010000141         1870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2055464, whole genome shotgun sequence.
ACCESSION   DATIMH010000141 DATIMH010000000
VERSION     DATIMH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055464"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VLI90_02370"
     CDS             <1..754
                     /locus_tag="VLI90_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HSV13076.1"
                     /translation="TKPVIYNVANFTKPAAGQPALLSFDDVTTMFHEFGHALHGMFAD
                     EQYPSLSGTNTARDFVEFPSQFNEHWALEPSVFANYAKHYKTGAPMPQALVDKIKKSA
                     TFNKGYDMTEAISAALLDMSWHTLPADAPKQDVDAFELAALKKNGTDLSYVPPRYSSS
                     YFMHIWGNGYAAGYYAYSWTQMLADDGYEWFKEHGGLTRANGDAFRRMVLSRGNTEDL
                     ATMYRNWRGHDPDIKPMLKDRGLAGGDEGKTP"
     gene            complement(837..1265)
                     /locus_tag="VLI90_02375"
     CDS             complement(837..1265)
                     /locus_tag="VLI90_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSV13077.1"
                     /translation="MDIRSVMTANPATCTPDATLRQAAQMMKQHDCGQIPVVGQDRQP
                     LGVITDRDIAVRAVAEGGDPASATVGDYMTSPVTTVPSSCSLDDVAKVMEQGQIRRVV
                     VIDDQGSVAGIVAQADIARAGRDGKTAEVVRQVSEPGRSQ"
     gene            1461..>1870
                     /locus_tag="VLI90_02380"
     CDS             1461..>1870
                     /locus_tag="VLI90_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13078.1"
                     /translation="MKHPLALALATALVLAAPAYATPAPAKQPAKHAAVNQTNPFFAD
                     SPLPLHYPQFDKIHDGDFAPAFDRGMAEQLKEIDAIANNPDAPTFDNTIIPLEKSGRT
                     LTRAATVFYNLVSADTNPTRDKIDAAYSSKFAAHR"
BASE COUNT          350 a          669 c          550 g          301 t
ORIGIN      
        1 caccaagccg gtcatctata acgtcgccaa cttcaccaag cccgccgccg gccagcccgc
       61 gctgctcagc ttcgacgacg tcaccacgat gttccatgag ttcggccacg cgctgcatgg
      121 catgttcgcg gacgagcagt atccgagcct gtcgggcacg aacacggcgc gcgacttcgt
      181 cgagttcccc tcgcagttca acgagcactg ggcgctggaa ccgagcgtct tcgcgaacta
      241 cgcgaagcac tacaagaccg gcgccccgat gccgcaggca ctcgtcgaca agatcaagaa
      301 gagcgcgacc ttcaacaagg gctacgacat gaccgaggcg atctcggccg cgctgctcga
      361 catgtcctgg catacgctgc cggccgatgc gccgaagcag gacgtggacg ccttcgaact
      421 cgcggcgctg aagaagaacg gcaccgacct gagctatgtg ccgccacgct acagttccag
      481 ctacttcatg cacatctggg gcaacggcta cgcggcgggt tattacgcgt attcgtggac
      541 gcagatgctc gcggacgacg gctacgagtg gttcaaggaa catggcggcc tgacccgtgc
      601 caacggcgac gcgttccgca ggatggtgct gtcgcgcggc aacaccgagg acctggccac
      661 gatgtaccgc aactggcgcg ggcatgatcc ggacatcaag ccgatgctga aggatcgcgg
      721 ccttgccggc ggcgacgagg gcaagacgcc ctgatcgtga cgttcaccgc tgcagaaaaa
      781 cgccgcccac caggcggcgt ttttcattgc aatcaccttt cgtaggagtg gtggctctat
      841 tgcgagcgac cgggttccga gacctgcctc accacctcgg ccgtcttgcc gtcgcggcct
      901 gccctggcga tatccgcctg cgccacgatg ccagcgacgg agccctggtc gtcgatgacc
      961 acgacgcggc gtatctgccc ttgctccatc accttggcca catcatcgag gctgcacgag
     1021 gacggcaccg tggtcaccgg cgatgtcatg taatcgccga ccgtggcgct tgccggatca
     1081 ccgccctcgg cgacggcacg cacggcgatg tcgcgatcgg tgatcacgcc caacggctgg
     1141 cgatcctggc ccaccacggg aatctggccg cagtcgtgct gcttcatcat ctgcgcggcc
     1201 tggcgcaggg tcgcgtccgg cgtgcaggtg gcgggattag cagtcatcac cgaacggatg
     1261 tccattggcg gctcctgtgt ctgggggaac gctcaccttg ccgcaaccat cgtcttggca
     1321 aagtgaatcg ccagcccacc gcctccgcgc gtcgcatcac ggatcagccg tgcaagcggc
     1381 cttaccgtgc ctttcgccat aggcggaccg gcgactccat cggctaggct gcccgttcac
     1441 ctacgaatgg aaccgttccg atgaagcacc cgcttgccct ggcgctcgcc accgcgctgg
     1501 tcctcgccgc gccggcctac gccacgcccg cccccgcaaa gcagcccgcc aagcacgccg
     1561 ccgtgaacca gaccaatccg ttcttcgccg acagccccct gccgctgcat tacccgcagt
     1621 tcgacaagat ccacgacggc gacttcgccc ccgcgttcga tcgcggcatg gctgaacagc
     1681 tgaaggaaat cgacgcgatc gccaacaacc ccgacgcgcc gacgttcgac aacaccatca
     1741 tcccgctcga gaaatccggg cggacgctga cccgcgccgc gaccgtgttc tacaacctgg
     1801 tcagcgccga caccaatccg acgcgcgaca agatcgatgc ggcctactcg tcgaagttcg
     1861 ccgcccaccg
//
LOCUS       DATIMH010000142         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4944112, whole genome shotgun sequence.
ACCESSION   DATIMH010000142 DATIMH010000000
VERSION     DATIMH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4944112"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VLI90_02385"
     CDS             <1..332
                     /locus_tag="VLI90_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV13079.1"
                     /translation="ASAVNWASLAEKIGRMRDSRCGIQQQAVAKIAHVRTATIQDIAD
                     GRREFSSREVSLIERACNRTGGELAALVLDPTGGALAVASMWADYARKFECRPVAVGI
                     AEPKAAG"
     gene            complement(329..1210)
                     /gene="miaA"
                     /locus_tag="VLI90_02390"
     CDS             complement(329..1210)
                     /gene="miaA"
                     /locus_tag="VLI90_02390"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSV13080.1"
                     /translation="MAIAEQVGGEILSVDSMQVYRRMDIGTAKPTPAEQARVRHHLID
                     LVDANESFTVARFVELADAVLAGAKTRNVPLIVTGGTPLYYKALFEGLFAGPTADETL
                     RQTLRALPADELHRRLSAVDPAAAARLHVNDTRRVIRALEVYEATGQPISSLQTEWTS
                     PKARHPAVWVGLSWDREVLNRRINARVKAMIAGGWLEETRQLIERFGQLSPTAGEATG
                     YRELIEHLQGKIALDDAVEQIKIATRQLARRQMKWFRRFRDVKWVMCVPEEWRDALPA
                     GLGEQICDHWQSAGRAT"
     gene            1203..1442
                     /locus_tag="VLI90_02395"
     CDS             1203..1442
                     /locus_tag="VLI90_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13081.1"
                     /translation="MAIDSADLPEAVGPIIARMGGVSGIVQNCQSGNDCHSCRKGQTS
                     GKSLCFASIGTALAYAHGAAAPSRHGLCSTFFGGI"
     gene            1487..1930
                     /locus_tag="VLI90_02400"
     CDS             1487..1930
                     /locus_tag="VLI90_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSV13082.1"
                     /translation="MALPTRVNRSQQNDPMEAMQREFDSMFGRYLTGRGGNDGGRLAP
                     YGVDVREDADHIYVEAELPGFKKDEVDITLENQTLTITAERKEHEQQKGDLLLNERRY
                     TRFLRSFTLPPTVDEQTVNAKLNDGVLTVTLNKREESKPRKISVS"
     gene            complement(2213..3211)
                     /gene="queG"
                     /locus_tag="VLI90_02405"
     CDS             complement(2213..3211)
                     /gene="queG"
                     /locus_tag="VLI90_02405"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HSV13083.1"
                     /translation="MIDAITSNSPPMIDIHALAGRVKAKAREIGFDLVGITTAEVSRY
                     RDYLRQWLDDGQAGTMNYLADRFAERTDPATYLPDAKSIICVAINYHAQLKPPTSEQG
                     VPGRIARYALGDDYHEIIKDRLHALADWLRAQVPGTRTRASVDTAPVMEKELAARAGV
                     GWLGKNTCIINEHTGSWILLGEVLTTLPLPPDDAAIDRCGTCTRCIDACPTAAITGPY
                     KLDARRCISYLTIEHREAIPPELSEKIGDWLYGCDICQDVCPWNKQAPLAVDPVLQPR
                     FPTGSADAEEVLTWNADDYRQRLRHSAMKRVKLPVLQRNARIAIENLSLKDTLGTK"
     gene            complement(3208..>3639)
                     /locus_tag="VLI90_02410"
     CDS             complement(3208..>3639)
                     /locus_tag="VLI90_02410"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HSV13084.1"
                     /translation="EDAMKEGKFANLQGMGQPLDLEPMPADENVRMTWWAIRILKNND
                     FTPHEVQWRKQIDKLREMIDRLTDESKLPQLVARVNELARQLNTLGTNAIRLPIASID
                     LEMERERLRERRSQLGKRHRDTGRSQPEARVTMKVLNPGNP"
BASE COUNT          647 a         1097 c         1160 g          735 t
ORIGIN      
        1 gcgcctcggc ggtgaactgg gcgtcgcttg ccgaaaaaat cggaaggatg cgtgatagcc
       61 gttgcggtat ccagcagcag gccgtggcca agattgccca cgtgcgaact gcgaccattc
      121 aggatatcgc cgacggtcgc cgcgaatttt ccagtcgcga agtttcgctg atcgagcggg
      181 cctgcaatcg aacgggtggt gagttggccg cgctggtgct cgacccgacc ggtggcgcgc
      241 tcgcggtagc gtccatgtgg gcggattacg cgcgaaagtt tgagtgccgc ccggtggcgg
      301 tgggcatcgc agagccgaag gcggcaggtt aggtcgctcg cccagcgctc tgccaatggt
      361 cgcaaatctg ttcgcctaag ccggccggca aagcatcacg ccactcctct ggcacgcaca
      421 tgacccattt cacatcccga aaccgccgga accacttcat ctgccggcgg gcgagttggc
      481 gggttgcgat cttgatctgc tcgaccgcgt cgtctagcgc gatctttccc tgcaagtgtt
      541 cgatcaattc acggtagccc gtcgcttcgc cggcggtggg cgagagttgg ccaaagcgtt
      601 cgatcagctg gcgggtttct tcgagccagc caccggcgat catcgccttc actcgagcgt
      661 tgatccggcg gttcagtacc tcgcgatccc atgacaggcc gacccacacc gccggatggc
      721 gcgctttggg cgacgtccat tcggtctgta gagatgagat cggctgtccc gtcgcctcgt
      781 acacctcgag cgcgcgaatg acgcggcgtg tgtcgttgac gtgcagacga gcggcggcgg
      841 cggggtcaac cgcgctgagc cggcggtgca actcatctgc cggcagggcg cgcagcgttt
      901 gtcgaagcgt ttcatccgcg gtgggtcctg cgaacaagcc ttcgaacaat gccttgtagt
      961 acagcggcgt tccaccggtg acgatgagcg ggacatttct cgtcttcgcg ccggcaagga
     1021 cggcgtcggc aagctcaacg aaacgcgcga ccgtgaatga ctcgttcgcg tcgacgagat
     1081 cgatgaggtg atgccgcacc cgagcctgct cggccggtgt gggtttggcg gtgccgatat
     1141 ccatcctgcg gtagacctgc attgaatcga cgctgaggat ttcgccgccg acctgttcgg
     1201 cgatggccat cgacagcgcg gatttgcctg aagcggtggg accgataatc gcgaggatgg
     1261 gcggcgtcag cggcattgtt caaaactgcc agagcggcaa cgactgtcat tcttgcagga
     1321 agggccaaac gtcgggcaaa tccttatgtt ttgcaagcat cgggacggct ttggcttacg
     1381 cgcatggggc cgccgcgccg tcgcggcatg gtctttgttc taccttcttc ggcggcattt
     1441 gactggatta gccgcacctt aaatggcaat gaaaggagaa ccaaccatgg cactgcctac
     1501 tcgagtcaat cgttctcaac agaacgatcc aatggaagcg atgcagcgcg agttcgacag
     1561 catgttcggc cgctatctaa ccggtcgcgg cggcaatgat ggtggccggc tggcgcccta
     1621 cggcgtcgac gttcgcgaag acgccgacca catctatgtt gaagcggagt tacccggctt
     1681 taagaaggac gaggtggata tcacgctgga aaaccagacg ctgacgatca ccgccgagcg
     1741 gaaggaacac gaacagcaga agggtgacct gctgctgaac gaacggcgtt acacccgctt
     1801 cctccgcagc ttcacgctcc cgccgacggt ggatgaacag accgtcaacg cgaagctgaa
     1861 tgacggcgtg ctcacggtca cgctgaacaa gcgcgaagag agcaagccgc ggaagatttc
     1921 ggtgtcgtaa tcgggtgtaa ctaaactttg tggtccggcc cttctctggt cccctcgccc
     1981 ggtactccgg gagaggggga cggaccagaa tagagccaca aattaaagtc acacccgtcg
     2041 taatcttggc cttgtcctga gcgcaggcgg taccccgccg gagtcgaagg atctagctgg
     2101 aagtgagtga acggtcatcc tgcgcgaaca ggcgcggcag tcgctcgcgg tttcgcggtc
     2161 tcctggtctg cgaaaccgca agcgactgcc gacgccgtct cgcgaacgcc gcttacttgg
     2221 ttccgagcgt atccttcaac gataaattct cgatggcgat ccgggcgttg cgctgcagca
     2281 ctgggagctt cacgcgcttc atggcgctgt gccgcaggcg ctgacggtag tcatccgcgt
     2341 tccacgtcag cacttcttcg gcgtcggccg atcctgtcgg aaacctcggt tgtaacaccg
     2401 ggtccaccgc caatggcgct tgtttattcc acgggcagac gtcctggcag atgtcgcagc
     2461 cgtagagcca gtcgccgatt ttttcgctta gctccggcgg gatggcttcg cgatgttcga
     2521 tcgtcaggta cgagatgcaa cgccgggcgt cgagtttata cggcccggtg atcgcggcgg
     2581 tcgggcacgc gtcgatgcaa cgcgtgcagg tgccgcagcg gtcgatcgct gcatcatccg
     2641 gcggcagcgg cagcgtggtc agcacttcgc cgagtaatat ccacgagccg gtgtgctcgt
     2701 tgataatgca ggtgttcttg ccaagccaac cgacgccggc gcgggccgcc aattcctttt
     2761 ccatgacagg agcggtgtcc acgctggccc gcgtccgcgt gccgggcact tgcgcgcgca
     2821 gccaatctgc cagtgcgtgc agccggtcct tgatgatctc gtgatagtcg tcgcccagcg
     2881 catatcgcgc gatgcggccg ggcacgcctt gctcactcgt cggcggtttc agctgcgcgt
     2941 gatagttgat cgcgacgcag atgatgctct ttgcatcggg cagataagtg gccggatccg
     3001 tgcgctcggc gaagcgatcg gccagatagt tcatcgtgcc ggcctggccg tcgtcgagcc
     3061 attggcgcag ataatcccga tagcgtgaaa cttcggcggt ggtgatgccg acaagatcga
     3121 agccgatttc gcgcgccttc gccttcaccc gcccggcgag cgcgtgtata tcgatcattg
     3181 gcggcgagtt tgaagtaatg gcatcgatca cgggttgccc ggatttagaa ccttcatcgt
     3241 gacacgggcc tccggctggc tccggcccgt gtcacggtgg cgttttccta gctgtgaccg
     3301 ccgttcgcgc aggcgctcgc gctccatctc cagatcgatc gacgcaatcg gcaggcggat
     3361 ggcgttggtg ccgagcgtgt tgagctggcg ggcgagttca ttcacgcgcg cgacgagctg
     3421 cggcagcttt gattcatccg tcaagcggtc gatcatttct cgcagtttgt cgatctgctt
     3481 ccgccactgc acctcgtgcg gcgtgaaatc gttgttcttc aggatgcgga tcgcccacca
     3541 ggtcatgcgg acgttctcgt cggcgggcat cggctcgagg tccagcggct ggcccatgcc
     3601 ctgaaggttg gcgaacttcc cctccttcat cgcgtcctc
//
LOCUS       DATIMH010000143         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_324748, whole genome shotgun sequence.
ACCESSION   DATIMH010000143 DATIMH010000000
VERSION     DATIMH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324748"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VLI90_02415"
     CDS             <1..964
                     /locus_tag="VLI90_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13085.1"
                     /translation="IPAECELWFYTNRNVPPGESTDLDVSLRISADTSMRHLLAPYKA
                     AFDQHFPSLRYHPDPRPVAQFAAVAPQLITFENPLGFNGAWRRFDTAIGTARFVDWMV
                     PRLLRANAAGCIFWTLGGYNPRGAMYRPDFDELPPAVMANVPALTRGFKSRGLRVGLC
                     ARPADIVRPHDATTDELYRISMDHPAEVEMVLNRFRHAMAMGFDVFYCDTFAMNDQDL
                     KLLQKIRDVVGPDVLLYTELGFDLTLPLAGRYCEWHENEVAGLSREEYEAMRYLCPDS
                     TWLAKSRTKEVVPAAYAKWGLVPLVEDFQTPMLPPATRPARTQR"
     gene            complement(1133..2287)
                     /locus_tag="VLI90_02420"
     CDS             complement(1133..2287)
                     /locus_tag="VLI90_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HSV13086.1"
                     /translation="MDFQQLLRFAVEHDASDVHIQAGLPPNLRIGGLLRAASVPPVAD
                     EELHNFIKSIAPARFRDNLDDRITTGLDFSYAMPGLSRFRCSAYRQLGLTGISMRVIK
                     SKIRSVEELHLPEVVNDIAVSSHGLTLVTGTTGSGKSTTLAAMIDLINQNHSSKIITI
                     EDPVEYLHTSKKALIAQLEVGTDTPSFEQALRQALRQDPDVVLVGELRDMDTLRIALR
                     AADTGHQVFSTVHSASAPQTIERIIAMFPPNEHKLLLTQLAGNLEAIISQRLVICRDG
                     SRRPATEILRGGPVPTKYILEGRALELGDYIRSAGNGQQTFDQDLLALHHQQLISHDE
                     ALKNATNREALSMALRGISSSKSITEPGRGAGGGSKTPTPPRPAPAGGHV"
     gene            2452..3108
                     /locus_tag="VLI90_02425"
     CDS             2452..3108
                     /locus_tag="VLI90_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13087.1"
                     /translation="MRFRKSKFAQLGLGTILVAAAVGATPASALAGRHDDFDRRDYRD
                     HDRGDLRIGLDLPGIVVEHRYTPPPCDPVETRVWVEPVYRTVCDRQWMPATYRTVCDR
                     VWVEPVTRTVCDRVYVPDRYEWRDVVRYEYGYRRVARERVLVEPAHYVEQPHQIVVTP
                     GHYQDVQRQELVCDGHWDNVEHQELVTPGHWETRERIVERPLYEEHAGVRLDLRFPIR
                     "
BASE COUNT          546 a          942 c         1036 g          607 t
ORIGIN      
        1 catcccggcc gagtgcgagc tttggtttta caccaaccgc aacgtgccgc cgggcgaatc
       61 gaccgatctg gacgtgagcc ttcgcatcag cgccgacacg tcgatgcggc acttgctcgc
      121 gccgtacaag gcggcgttcg atcagcattt tccgtcgctg cgctatcatc ccgatccgcg
      181 cccggtggcg cagttcgcgg ccgtcgcccc gcagttgatc accttcgaaa acccgctcgg
      241 cttcaacggc gcgtggcggc gtttcgacac cgccattggg accgcgcgct tcgtggactg
      301 gatggtccca cgactgctcc gcgccaatgc ggccggttgc atcttctgga ccttgggcgg
      361 ctacaacccg cgcggggcga tgtaccgccc ggacttcgac gagctgccgc cagcggtgat
      421 ggccaacgtg ccggcgctga cgcgcggatt caaatcgcgc ggcctgcgcg tcggcctgtg
      481 cgcgcgaccc gctgatatcg tccgcccgca cgatgcgacc accgacgaac tgtatcgcat
      541 ttcgatggac cacccggcgg aagtggagat ggtgctgaac cgcttccgcc acgcgatggc
      601 gatgggcttc gacgtctttt attgcgacac gtttgccatg aacgatcagg acctgaagct
      661 gctgcaaaag atccgcgatg tggtcgggcc cgacgtgctg ctgtacaccg agctgggttt
      721 tgacctgacg ctgccgctgg ccgggcggta ttgcgagtgg cacgagaacg aagtcgcggg
      781 gctgagccgc gaggagtacg aggcgatgcg ctacctgtgc cccgactcga cctggctggc
      841 caaatcacgc acgaaagaag tcgttcccgc ggcgtacgcg aagtgggggc tggtgccgct
      901 ggtggaagat tttcaaacgc cgatgctacc accggcgacg cgcccggcga ggacgcaacg
      961 ttgatcttac acgccgggtt cgtggtcagt tgtcagttgt cagttgtcag ttgtggtata
     1021 cgcgacacac aaccgagtcc tgcatcccgg caccccatcc aaatccatcc gggtcgttgg
     1081 agtacctccg aagccggcgt ggtcgactgc ttcagtttct ccagaagcac ggtcacacat
     1141 gcccgcctgc cggggcgggg cgtggcggcg tcggcgtctt cgatccaccg ccggcgccgc
     1201 ggccgggttc ggtgatcgac ttgctcgagc tgatgccgcg tagcgccatc gacagcgctt
     1261 cgcggttcgt cgcgttcttc agcgcttcat cgtgcgagat caattgctgg tgatgcagcg
     1321 cgagtaggtc ttggtcgaac gtctgctggc cattgccggc gctgcggatg tagtcgccga
     1381 gttccagcgc acggccttcg aggatgtatt tggtcggcac cggtccgccg cgcagaattt
     1441 cggtggcggg gcggcgcgaa ccgtcgcggc agatgaccag gcgctgtgag atgatcgctt
     1501 cgaggttgcc ggccaactgc gtcagcagca gcttgtgctc gttgggcggg aacatcgcga
     1561 tgatgcgctc gatcgtctgc ggcgcgctgg ccgagtgcac cgtcgaaaat acctggtgcc
     1621 cagtgtccgc tgcccgcagc gcgatgcgca gcgtgtccat gtcgcgcagt tcgccgacca
     1681 acacaacgtc cggatcctgc cgcagcgcct gccgcaatgc ctgttcaaaa ctgggcgtat
     1741 cggtgccgac ttccaactgc gcgatgagcg ccttcttcga cgtgtgcagg tactcgaccg
     1801 gatcttcaat cgtgatgatc ttcgacgaat ggttttgatt gatcaggtcg atcatcgctg
     1861 ccagcgtggt cgatttgccg gaaccggtgg tgccggtgac gagcgtgaga ccgtgactcg
     1921 acacggcgat gtcgttcacc acctccggca gatgcagttc ttctaccgag cggatcttgc
     1981 tcttgatcac gcgcatcgaa atgccggtca gacccagttg gcgatatgcc gagcagcgga
     2041 agcgcgacag gcccggcatc gcgtaggaga aatccaatcc agtcgtaatg cggtcatcca
     2101 gattatcgcg gaagcgcgcc ggcgcgatcg acttgatgaa gttgtgcaac tcctcatcgg
     2161 cgactggggg caccgacgcg gcgcgcagta acccaccaat gcgcagattt ggcggcagcc
     2221 ccgcctggat gtgcacgtcc gaagcatcgt gctcgaccgc aaagcgcagc aattgttgaa
     2281 agtccatatg ccctcgggaa gagttgaaaa acggagcgga cttatagcca ctctaccggc
     2341 cgggcccact ccgcgaaagc gggtcgtaag tggcggtgat tcgacaagat agctgaaact
     2401 aacttcgctc gccggctcgt gtctctcctg atgacttcaa aggagcgagc tatgcggttt
     2461 agaaaatcaa aattcgcaca actgggcctt ggcacgatcc tggtggcggc ggcggttggg
     2521 gcgacgcccg cgtccgcctt ggccggacgg cacgatgact tcgaccggcg tgattatcgc
     2581 gaccacgatc gcggcgatct gcgcatcggc ttggatctgc cgggcattgt ggtcgaacat
     2641 cgctacacac cgccaccgtg tgatccggtg gagacgcgcg tgtgggtgga gccggtctac
     2701 cgcaccgttt gcgatcgcca atggatgccg gcgacgtatc gcaccgtttg tgatcgcgtg
     2761 tgggtcgagc cggtgacgcg cacggtgtgc gatcgcgtgt acgtgccgga tcgatatgag
     2821 tggcgcgacg tggtgcgata tgagtacggc tatcgccggg tggcgcgcga gcgcgtgctg
     2881 gttgaaccgg cgcattatgt tgagcagccg caccagatcg tcgtcacccc ggggcattac
     2941 caggatgtgc agcggcagga gttagtgtgt gacgggcact gggataatgt ggaacaccag
     3001 gagctggtga cgccggggca ttgggagacg cgcgagcgta tcgtcgagcg gcccctgtat
     3061 gaagaacacg cgggcgtgcg cttggatttg cggtttccga tccgatgaaa tgccagcgcg
     3121 gcgggggggg g
//
LOCUS       DATIMH010000144         4630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2634691, whole genome shotgun sequence.
ACCESSION   DATIMH010000144 DATIMH010000000
VERSION     DATIMH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634691"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2236
                     /gene="glgP"
                     /locus_tag="VLI90_02430"
     CDS             <1..2236
                     /gene="glgP"
                     /locus_tag="VLI90_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HSV13088.1"
                     /translation="YFSAEFGLHESLPVYSGGLGILAGDHLKSAAELAIPLTAVGLLY
                     RNGYFQQYLSNDGWQQEAYPELDFYNLAVEQQKYTDGTPVRVRVDLPDNAVFCNVWKV
                     NVGRIPLYLLDTNLAENSPADREITSRLYGGGTEMRIKQEIVLGIGGCRALEAMNVTP
                     TVYHMNEGHSAFSALERIRVLLEQHPTLTFDEVRQEVMATSIFTTHTPVPAGIDMFPP
                     ELMLKYFKNYIPSLKLDDEGFLALGREDVTNKKQGFSMAVLAIRLADGINGVSWLHGD
                     VSRHMWHNLWPQVPPSDVPIKHVTNGIHVRSWLSADIAFTLDRYLGEGWMSDPADHSV
                     WEGVAQIPDEELWRAHERCRERLVSWTRQTLREQMLRRGAAYDDLAAADEVLDPEALT
                     IGFARRFATYKRGALLLRDVERLKKLLEDTKRPIQFIFAGKAHPADQEGKELIRAIVN
                     FARSTPQIRRRMVFIENYDINIARQLVQGVDVWLNTPRRGMEASGTSGMKAAANGVPN
                     CSILDGWWVEGYSPDVGWAIGRGESYGDAGYQDHVESMALYDLLEKQIIPSFYQRGVD
                     NIPREWIARMKNCMRKLAPVFNTNRMVREYSERFYIPALDRGEQLAADNLARAVNLAH
                     AKDALRQHWGKIKIVGVHTTGNGHYKVGESVQVETLVDLPDVKPDEVTVQLYCGPTTA
                     AGELENPQALVMQHVKELAPNRHVFTGRIECRTSGRQGFAVRILPGNRDLATPFEPGM
                     IIWN"
     gene            2419..4164
                     /locus_tag="VLI90_02435"
     CDS             2419..4164
                     /locus_tag="VLI90_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSV13089.1"
                     /translation="MRGPFRHWLLLLALLALFAAFMLWPIAQIVRVGFQGIPTDGHYG
                     AFTLAYIAAVFKDPQLRRGLINSAAIAIAVTALCTLLSLPLALLTRRYDFRGKSLVNG
                     LLLVPLVLPPFVGAIGMRQILGRFGALTALIEHMGLIAPNAPVDWLGAAPLPGIILVE
                     ALALYPILYLNLTAALANLDPAMEQAAANLGASRWRILRTITLPLVRPGLFAGGTIVL
                     IWSFTDLGTPLMFNYTDVTPVQIWHQINQVSGNPIPYALVVVMLLASATLYVIGRLLL
                     GRGHEVAVTKAATTAVVAEPLGLLRSIPILLPFVLVISLALLPHVGVILTSISGVGAW
                     YRSVLPQTLTARHYVNALTYDVTLASMRNSVMFASLSVLLDIALGLAVAYLIVRSHLP
                     GRRMIDALAMLPLAVPGLVLAFGYLAISLQLQAWFKNAHWLREMVDVTENPTLLLVIA
                     YAMRRLPYVVRSAVAGLQQTPVDLELAARNLGASSAATLRRITVPLISANLIAGALLA
                     FAFAMLEVSDSLILAQKETYWPITRAIYELFQRLGDGPYIASALGVWAMVLLALTILS
                     ANSLLGRRLGAAFRV"
     gene            complement(4177..>4630)
                     /locus_tag="VLI90_02440"
     CDS             complement(4177..>4630)
                     /locus_tag="VLI90_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13090.1"
                     /translation="DCSDYGIPVKPTRIARGLSWVMLGFGLRDRCGSTVSVSVDPAST
                     KSVEGVLVMDRPMHTMAANDDPRLPEPLGQDFLRQSVHVLEWAPRRCLIPSPTHSWER
                     AGVRVISNIERLLKDTNHPHPNPTLTLSRRTGRGDQKRKTGAVGANVN"
BASE COUNT          818 a         1539 c         1419 g          854 t
ORIGIN      
        1 ctacttctct gctgagttcg gcctgcacga atcgctgccg gtttattccg gcggtctcgg
       61 catcctcgcc ggtgatcatc tgaagagtgc cgccgaattg gccatcccgc tcaccgcggt
      121 gggcctgctt tatcgcaacg gatatttcca gcaatacctc tccaatgacg gctggcagca
      181 ggaagcgtat cccgagctgg acttctacaa cctcgccgtc gagcagcaga agtacaccga
      241 cggcacgccc gttcgcgtgc gcgtcgactt gccggacaac gcggtcttct gcaacgtgtg
      301 gaaggtgaac gtcggccgaa tcccgctgta cctgctcgac acgaacctgg ccgaaaattc
      361 cccggccgat cgggagatca ccagccgcct ctacggcggc ggcaccgaga tgcgcatcaa
      421 gcaggagatc gtcctgggca tcggtggttg ccgcgcgctc gaggcgatga acgtcacgcc
      481 gaccgtctat cacatgaacg aaggccactc cgccttcagc gcgctggagc gcatccgcgt
      541 gctgctcgag cagcatccga cgctgacctt cgacgaagtg cgccaggaag tgatggccac
      601 cagcatcttc accacgcaca cgccggtccc cgctgggata gacatgttcc cgccggagct
      661 gatgctgaag tacttcaaga actacatccc gtcgctgaag ctggatgatg agggatttct
      721 cgcgctcggc cgcgaagatg tgaccaacaa gaagcagggc ttttcgatgg cggtgctcgc
      781 catccgcctc gccgacggca tcaacggtgt ctcctggttg cacggtgatg tgagccggca
      841 catgtggcac aacctatggc cgcaggtgcc gcccagcgat gtgccgatca agcatgtcac
      901 caacggcatt cacgtgcgtt cctggctgtc ggccgacatc gcgttcacgc tcgatcgtta
      961 tctcggtgaa ggctggatga gcgaccccgc cgaccacagc gtgtgggaag gcgtcgcgca
     1021 gattcccgat gaagaactgt ggcgcgccca cgagcgctgc cgcgagcgcc tggtcagctg
     1081 gacgcgccag acgctgcgcg aacagatgct gcggcgcggc gccgcctacg acgatctcgc
     1141 cgccgccgat gaagtgctcg atcccgaagc gctcacgatc ggcttcgcgc gtcggttcgc
     1201 gacgtacaaa cgcggcgcgc tgctgctgcg cgatgtcgag cggctgaaga agctgctgga
     1261 agacacgaag cgtccgatcc agttcatctt cgccggcaag gcgcatccgg ccgaccagga
     1321 aggcaaggag ttgatccgcg cgatcgtcaa cttcgcccgc agtacaccgc agatccgccg
     1381 gcggatggtc ttcatcgaaa actacgacat caacatcgcc cggcaactgg tgcagggcgt
     1441 ggacgtctgg ctcaacaccc cgcgccgcgg catggaagcc agcggcacca gcggtatgaa
     1501 ggccgccgcc aacggtgtgc ccaattgctc catcctggac ggttggtggg tcgaaggcta
     1561 cagccccgac gtcggctggg cgatcggccg cggtgagagc tacggcgacg ccggctacca
     1621 ggaccacgtc gagtcgatgg cgctgtacga cctgctggaa aagcagatca tcccgtcctt
     1681 ttatcaacgc ggcgtggata acattccgcg cgagtggatc gcccgcatga aaaactgcat
     1741 gcgcaagctc gccccggtct tcaacaccaa tcgcatggtg cgcgaataca gcgagcgctt
     1801 ctacatcccc gcccttgacc gcggcgaaca gctggccgcc gacaaccttg cccgggcggt
     1861 caacctcgcc cacgcgaagg acgcgcttcg ccagcattgg ggcaagatca agatcgtcgg
     1921 cgtgcacacg accggcaacg gtcactacaa ggtcggcgaa agcgtgcagg tcgaaacgct
     1981 ggtcgatctg ccggatgtga agccggacga agtcacggtg cagctctatt gcggccccac
     2041 aacagcggcg ggggagttgg aaaacccaca ggcgctggtg atgcagcacg tgaaggagtt
     2101 ggcgccaaac cgccacgtgt tcacaggaag aatcgaatgc cgcaccagcg gcagacaggg
     2161 attcgccgtg cgaatcctgc cgggcaaccg cgatttagcg acgccattcg aacccggcat
     2221 gatcatctgg aactaacgct ctggtcccct cgcccggtac gccgggagag ggctagggtg
     2281 agggtgattt tgcttttcga gtatcgctcg acgctcgaaa tcaccctcac ccccgaccct
     2341 tccccgagta ccgggcgagg gagagatgct gaagcacgtc gatctgattt ccatcgcgct
     2401 cggttatcct tcccgcccat gcgcggtccg ttccgacact ggctcctgct cctggcgctg
     2461 ctcgcgctct tcgccgcgtt catgctctgg cccatcgcgc agatcgtacg cgtgggcttc
     2521 cagggcatcc ccaccgatgg ccactacggc gccttcaccc tcgcctacat cgcggcagtt
     2581 ttcaaagacc cgcagctccg ccgcgggttg atcaacagcg ccgccatagc gatcgccgtc
     2641 acggcgctgt gtaccctatt gagcctcccg ctcgcgctgc tcacccgccg ctacgacttc
     2701 cgcggcaaat cgctggtcaa cggcctgctc ctcgtcccgc tcgtgctccc gccgttcgtc
     2761 ggcgcgatcg gcatgcggca aatcctcggc cgcttcggcg cgctgaccgc gctgatcgaa
     2821 cacatgggcc tcatcgcgcc gaacgctccc gttgactggc tcggcgccgc gccgctgccc
     2881 ggcatcatcc ttgtcgaagc gttggctctc tacccgatcc tctacctcaa cctcaccgcc
     2941 gccctcgcga accttgaccc cgcgatggag caagcggcgg caaacctcgg cgcatcgcgc
     3001 tggcgcatcc tccgcacgat cacgctgccg ctggtccgcc ccggcctctt cgccggcggg
     3061 acgatcgtgc tgatctggag cttcacggac ctgggcacgc cgctgatgtt caactacacc
     3121 gacgtcacgc ccgtgcagat ctggcaccag atcaaccagg tatctggtaa tccgatccca
     3181 tacgcgctgg tcgtcgtgat gctgctcgcc agtgcgacgt tgtacgtgat cggccggctg
     3241 ctgctcggcc gcgggcatga ggtcgcggtg accaaggctg cgacgaccgc cgtcgtcgcc
     3301 gaaccgctcg ggttgctgcg cagcatcccg atcttattgc cgttcgtgct ggtgatctcg
     3361 ctggcgctgc tgccgcacgt cggcgtgatc ctcacgagca tcagcggcgt cggcgcgtgg
     3421 tatcgttcgg ttctgccgca aacgttgacc gcgcgacatt acgtcaacgc gctgacgtac
     3481 gacgtcacgc tagcgtcgat gcgcaacagc gtgatgttcg cgagcctcag cgtcctgctc
     3541 gacatcgcgc tgggcctggc ggtcgcgtac ctgatcgtcc gctcgcacct gccgggccgg
     3601 cggatgatcg acgcgctggc gatgctgccg ctggccgtgc cggggctggt gctcgccttc
     3661 ggttatttgg cgatcagcct gcaactgcag gcgtggttca aaaacgcgca ctggctgcgc
     3721 gagatggtcg acgtcacgga aaatcccacg ctgttgctgg tcatcgcgta cgcgatgcgg
     3781 cggctgccgt acgtcgtccg cagcgctgtt gccggcctgc aacaaacgcc ggtggacctg
     3841 gaactggcgg cgcgaaatct cggcgcgtca tcggccgcaa cgctgcggcg gatcactgtt
     3901 ccgctgatca gcgcaaacct gatcgccggc gcgctgctcg cgtttgcgtt tgcgatgttg
     3961 gaggtgagcg attcgctgat cctggcgcag aaggaaacct actggccgat cacgcgcgca
     4021 atctacgaac tgttccaacg cctcggcgac ggcccgtaca ttgccagcgc gctgggcgtc
     4081 tgggcgatgg tcctgctggc gctgacgatc ctgtcggcga attccttgct aggccgccgc
     4141 ctcggcgccg cgtttcgggt ttgatgactg ctcttgtcaa ttgacgttcg cgccgacagc
     4201 gccggtcttt cttttctgat cccctcgccc ggtacgccgg gagagggtta gggttgggtt
     4261 agggtgaggg tgatttgtgt ctttcaataa ccgttcgatg ttcgaaatca ccctcacccc
     4321 agccctctcc cacgagtgcg tgggcgaggg gataagacaa cgtcgtggcg cccattctaa
     4381 aacgtgaacc gactgccgaa ggaaatcctg gcccagcggc tcgggcaaac gagggtcgtc
     4441 gttggcagcc attgtgtgca tcggtcggtc catcaccagc acaccttcga ctgacttggt
     4501 tgacgcggga tcgacggaaa cgctgacggt cgatccacat cgatcgcgaa ggccgaagcc
     4561 gagcatcacc caacttaagc cgcgagcgat gcgggtgggc ttgactggaa tgccgtagtc
     4621 cgagcaatcg
//
LOCUS       DATIMH010000145         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_614568, whole genome shotgun sequence.
ACCESSION   DATIMH010000145 DATIMH010000000
VERSION     DATIMH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614568"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VLI90_02445"
     CDS             complement(<1..411)
                     /locus_tag="VLI90_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HSV13091.1"
                     /translation="MRIHNAFIKAGVAVGLLLLLAGSALRAETPATQSSSAKLTKVAC
                     VGDSITYGSRIEDRAHDSYPAQLGRMLGDQYNVRNFGVSGATLLKKGDRPYNKTKEYQ
                     PALDFAADIVVIMLGTNDSKPQNISHKDEFVADYD"
     gene            complement(435..758)
                     /locus_tag="VLI90_02450"
     CDS             complement(435..758)
                     /locus_tag="VLI90_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV13092.1"
                     /translation="MISARALYGILSRHGFEVTLASTIAQALDHLQIAQPDYVLLDLM
                     LPDGNGAAVLRHMRDIDHAARVVVTTAVSDPERLREVRDLKPDAVLHKPLDVNLLLMM
                     IGPTN"
     gene            complement(766..1980)
                     /locus_tag="VLI90_02455"
     CDS             complement(766..1980)
                     /locus_tag="VLI90_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSV13093.1"
                     /translation="MGWLNAINAWLTGGSTPYMTLFHCMSHDTLWITVTVTLDLLVAT
                     GYGLITLHWWRNQQLLPLSPARSALGNMRNIFTFCCICGYLFIPIKMFWPAWRLYDVF
                     LLALVFFTWRYALNAKSLRVIYSAIGRSNQLAEDLAKSRDESKRKSFFLNAISHDLRT
                     PLNGLMLHANLAEMSAASGDDAATRESIEEIKTCARHTADLLDGLLEYARLENADDSI
                     NESTFELDHMLRGIRSTHAPGAARKSLALEVTPSSGIVLRTDRLKLERILNNLLTNAV
                     KFTRSGSIRVEVERSSESLRLHVIDTGDGIAPEHVARLFEEFFQVHNHERDRQKGFGL
                     GLAISRRLVRRLGGNLDVQSELDRGSRFTVVLPAAVIVDPTKPTIAGASAGSEPIADA
                     QPRNDASAGVAH"
BASE COUNT          408 a          735 c          754 g          458 t
ORIGIN      
        1 gtcgtagtcc gccacgaatt catccttgtg cgagatattc tgcggcttgc tgtcgttcgt
       61 gcccagcatg atgaccacga tgtcggcggc gaagtccagg gccggttgat attccttggt
      121 cttgttgtac gggcggtcgc ccttcttcag cagcgtcgcc ccgctgacgc cgaagttgcg
      181 gacgttgtac tgatcgccca gcatgcggcc cagctgagcg ggataactgt cgtgcgcgcg
      241 atcctcgatg cgcgagccgt aggtgatgct gtcaccaacg caggccacct tggtgagctt
      301 cgccgacgac gattgcgtcg ctggggtttc ggcgcgaagc gcagatcccg ccagcagcag
      361 caacaggcca actgccacgc cggctttgat gaacgcattg tgaattcgca tgtcgtctcc
      421 gcgcgctatg cgccttagtt ggtcggtccg atcatcatca gaagcaggtt cacgtcgagc
      481 ggcttgtgca gcaccgcgtc gggcttgagg tcgcgcactt cgcgcagccg ctcgggatcg
      541 ctgacggccg tcgtgacgac gacgcgcgcc gcgtgatcga tatcgcgcat gtggcgaagc
      601 accgcggcgc cgttgccgtc gggcagcatc aggtccagca gcacgtagtc cggttgagcg
      661 atctgcagat gatccaacgc ctgcgcgatg gtgctcgcca gcgtcacttc aaatccgtgg
      721 cggctcagga tgccatacag cgcgcgagcg ctgatcatgt catcttcaat gagcaacacc
      781 cgccgaggcg tcgttgcgcg gctgggcgtc ggcgatgggt tcggatccgg cgctagcgcc
      841 tgcaatagtg ggcttggtgg gatcgacgat gacggcggcg ggaagaacca ccgtgaaccg
      901 gctgcctcga tccaattcgc tctgcacgtc gagatttcca cccaggcggc gcacgagtcg
      961 ccggctgatg gccaggccca gtccgaaacc cttttgccgg tctcgctcat ggttgtggac
     1021 ctggaagaat tcctcgaaca gccgcgccac gtgctcgggc gcgatgccgt cgcccgtgtc
     1081 gatcacgtgc agccgaaggc tttcactcga tcgctcgacc tcgacccgaa tcgacccgct
     1141 tctggtgaac ttcaccgcgt tggtcaggag gttattcaga atgcgttcca acttcaaccg
     1201 gtcggtgcgc aggacgatgc cggagcttgg cgtgacttcg agcgcgagcg atttacgagc
     1261 cgcccccggc gcgtgcgtgc tgcggatgcc gcgaagcatg tggtccaact caaaggtaga
     1321 ttcgttgatg ctgtcgtcgg cgttttccag ccgggcgtat tcgagcaggc cgtccagcag
     1381 atcggcggtg tggcgggcgc aggtcttgat ctcctcgatc gactcgcgcg tcgccgcgtc
     1441 gtcaccgctg gcggcgctca tctccgccag gttcgcgtgc agcatcaggc cattcagcgg
     1501 tgtgcgcagg tcgtggctga tcgcgttcag gaagaaactc tttcgcttcg attcgtcgcg
     1561 cgacttggcc aaatcttcgg ccagctggtt cgaccgcccg atggccgagt agatcacgcg
     1621 aaggcttttg gcgttgagcg cgtagcgcca ggtgaagaag accagcgcga gcaggaagac
     1681 gtcgtacagc cgccaggccg gccagaacat tttgatcggg atgaagaggt acccgcagat
     1741 gcagcagaac gtgaagatgt tccgcatgtt gcccagcgcg ctgcgcgccg gcgacagcgg
     1801 cagcagctgc tggttacgcc accagtgcag cgtgatcagc ccgtaacccg tggcgacgag
     1861 cagatcgagc gtcaccgtca cggtgatcca cagcgtgtcg tgcgacatgc agtgaaacag
     1921 cgtcatgtac ggggttgacc caccggtcaa ccaagcgttg atcgcgttca gccagcccat
     1981 accgctcgcc tttggaagta ccctgaaatg gcagcgcgct gcgcgcaccg gcccaccgtg
     2041 gccggcatcg tgcggcgcgc ttatcaaccg tcctacgtat aatgccgcgg ccggttcgca
     2101 tcataaaatc cctgatgcgg cggcgtttca cgccagggta aacgcatgta acaaccgcgc
     2161 gctctggtcc cctcgcccgg tacgccggga gagggttagg gccgccatct gttgcgatgc
     2221 cgaaaaagcg cacccccgct gccccgccga ctgccctggg ttcgccggtg ggtgccggtt
     2281 aggggtttgg tcgttcgcgg atcgatcgaa tatataattc ggccaatgct gccacttcac
     2341 gcgcgacgcc cgaag
//
LOCUS       DATIMH010000146         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5523242, whole genome shotgun sequence.
ACCESSION   DATIMH010000146 DATIMH010000000
VERSION     DATIMH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5523242"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VLI90_02460"
     CDS             <1..316
                     /locus_tag="VLI90_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HSV13094.1"
                     /translation="NHLPWTSEFREEIAELMRKGVELEYRYAEDTMPRGVLGLNAPMF
                     KKYLRFIANRRCQQIGLDPLFEKADNPFPWMAEMIDLKKEKNFFETRVIEYQTGGALS
                     WE"
     gene            1141..>1779
                     /locus_tag="VLI90_02465"
     CDS             1141..>1779
                     /locus_tag="VLI90_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13095.1"
                     /translation="MKRIIRYRGRFVLVALALAAGGLLSDLTYPAAHAASQASANANE
                     HKNMQLVGFNDLQGRSAYQPTIHHYTDGRYIAFVGHHGGTSPTVGGSNGTSIVDVTDP
                     SRPQYLFHIPGGAGTGEAGGAQMVRVCDGLAGTPGAGRVWMLRATSTSHELYDVTNPA
                     LPGAAIHVAGPFDATHKNEWECSTGIAYLVSAVPGWRVSRVTQVFDLSDPTNP"
BASE COUNT          332 a          525 c          554 g          368 t
ORIGIN      
        1 caaccatctt ccgtggacga gcgaattccg cgaggagatc gccgagctga tgcgcaaggg
       61 tgtcgagctc gaataccgct atgccgagga caccatgccg cgcggcgtgc tcggcctcaa
      121 cgcaccgatg ttcaagaagt acctgcgttt cattgccaac cgccgttgcc agcagatcgg
      181 cctcgatccg ttattcgaga aggccgacaa tccatttccc tggatggcgg agatgatcga
      241 tctcaagaag gagaagaact tcttcgagac ccgcgtcatc gagtatcaaa ccgggggcgc
      301 gctgtcctgg gaatgagtca cccaggcccg gaaacaacaa caacgaccat cgtctcaaag
      361 ggagaaattc cgtcctaatc gctgtggagg gaatgagatg gttgagttcc tttgggtgtg
      421 ggttgcggct ggcggtgggc tggctgtggg gatgtttttg tttgctgtga tgacgatggc
      481 ggcgaatgat ccgcaatcgg aggttgttag cgcttcgcat actgatgccc tgacgtagtt
      541 cttctcgctg caagggcgag tcactcgcgc acaactcgcg aggatagatc ttcgttctat
      601 tcgactgttc aaagcagggt gcgtagtgtc agctgcgtag gcgccggaga gtcgcgcttg
      661 cctcgacggc ttgaattcgc ctgccgaccc cacgcatttc gtggatcggc gtgtactgcg
      721 ccgctgccgc tgcctccgcg ccgtgtgcca gttaacgccg tcgagcttgc cattgcgccc
      781 gtccacgtat cggtcgggaa gcgggtgctt atgcgcgact gccattgttg attgattggg
      841 ttggcgtcga ggcgcgaagc gcttggtgtc ggatccatca aattggggat tgacacgcct
      901 tctgattcgc gccactctgg cgcgccgctt gtcgtgtaca gatcgtcttc gagtccaaac
      961 aggcaaagca tttcacgacg ttgctcgtcg ttgctctgta cgcgttcagt ggaaggtcgc
     1021 cggtgatggc ggacccggcc gaccgacgca cgctgctcgt ccgggccgag aaaaaacgcg
     1081 cccgcacagc ggggcagcat gcaagcgaat ccgtcgttct tgtctcagag agggctatcc
     1141 atgaaaagaa tcattcggta ccggggacgg ttcgtcctcg tcgcacttgc cttggctgct
     1201 ggcggcctgc taagcgattt gacgtatccc gccgcgcatg ctgcctcgca ggcatcagcg
     1261 aacgcgaacg aacacaagaa catgcaattg gtcgggttca acgacctgca gggtcggtcc
     1321 gcctatcagc cgaccatcca ccattacaca gacggccgct acatcgcctt cgtcggccac
     1381 catggcggca catcgccgac cgtgggtgga agcaacggca cgtcgatcgt cgatgttacc
     1441 gatccatcgc ggccccagta cctgtttcac atcccgggtg gcgccggcac aggcgaagca
     1501 gggggcgcgc agatggtgcg tgtgtgcgat ggtctggccg gcactcccgg agcgggccgt
     1561 gtctggatgc ttcgtgcgac ttcgacgtcg cacgagctct acgacgtcac aaacccggcc
     1621 ttgccagggg ctgcgatcca cgtggcgggc ccctttgacg ccacacacaa gaacgagtgg
     1681 gagtgctcga ccggcattgc gtatctcgtc tctgcggtgc ccggctggcg ggttagccgt
     1741 gtaacgcagg tattcgacct gagcgatccc accaacccg
//
LOCUS       DATIMH010000147         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1192176, whole genome shotgun sequence.
ACCESSION   DATIMH010000147 DATIMH010000000
VERSION     DATIMH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192176"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VLI90_02470"
     CDS             complement(<1..498)
                     /locus_tag="VLI90_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HSV13096.1"
                     /translation="MPGLSYESSGVDYDKLDAFKRACQQAAATTTDALRLHGLSEPAG
                     VRGESAYLMETPGSFLAHVEEGLGTKNLVADEMFRLSGKSYYANIGIDTVATIVNDLV
                     TVGALPISVAMHAAVGDSSWFDNGELAADLARGFAEGCRQAQAVWGGGETPTLRGIVD
                     PATIVL"
     gene            complement(638..>2098)
                     /locus_tag="VLI90_02475"
     CDS             complement(638..>2098)
                     /locus_tag="VLI90_02475"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HSV13097.1"
                     /translation="ADDGNYTLKLYFMERVKTAVGQRVFNVQAEGATVLSNLDIYGQV
                     GYHKALVKSVPVSVTGGALNLTFQSITDYAICSAIELDPVDAQPSAPAASVTLTAAAG
                     TNRVDLSWPDSSNNEAGFELERQGPGDASFIRIATLPSNVSSYSDTAVTAGASYSYRV
                     RAINNAGASAYSSVAQATVGQANPSAFTKVSWTTVQPSPVPRSEAQTAVVNGKLYTFG
                     GYLDGTTLVPAARSDVYDPATNQWTPVAPMLVGLTHSGCCNDGRYVYFAGGYSGPGGG
                     GNQTFARTNVMRYDTVTNSWSTLPGLPQARGAGGLVLLGRVLHFFGGTDLARQDRSEH
                     WTFDLDHDTAWHVAAALPTVRNHLGAVVLNGRIYAIGGQQHQDAKEVPQSAVQMWDPA
                     NPGVWTTLTPLTQARSHIAGATFVMNGRIIVLGGETTYLHGVSTCSAYDPTTNQWVDL
                     SPLSVARSSGNAGVINGVLYYSAGGLQKTVYKGVPG"
BASE COUNT          366 a          750 c          610 g          372 t
ORIGIN      
        1 cagcacgatg gtcgccggat cgacaatacc ccgcagcgtc ggcgtctcgc ctccgcccca
       61 caccgcctgc gcttggcgac aaccttcggc aaacccgcgc gccaggtcgg ccgccaattc
      121 gccattgtcg aaccacgatg aatcgcccac cgccgcgtgc atcgcgacgg agattggcag
      181 cgcgccaacc gtaacaagat cgttcacgat cgtcgcaacg gtatcgatgc cgatgttcgc
      241 gtaataactt ttgccgctca ggcggaacat ctcgtcggcg acgaggttct tcgtgccgag
      301 cccctcttcc acatgagcca gaaacgatcc cggcgtctcc atcagataag cgctttcacc
      361 gcgcacgcca gccggctcgc tcaggccatg cagccgcagc gcatcggtag tcgtcgccgc
      421 cgcctgctga caggcgcgct taaacgcgtc gagcttatcg taatcgacgc cgctggattc
      481 gtagctcaga cccggcatga cgcgtgttgt agggacacac gcgggttggg tcaaaccgca
      541 gcgccaccga tagccttgga actcagcacg atggcattct tcccgaaggt atgtagctgc
      601 gacgcaggcg tcgcagctac tgatctatca atactcccta ccccggcacg cccttataga
      661 cggtcttttg cagcccgccg gcggagtaat acaggacgcc gttgatcaca cccgcgttgc
      721 cggagcttcg ggcgaccgac agcgggctca gatccaccca ctggttcgtc gtcggatcat
      781 atgccgagca ggtggacacg ccgtgcaggt acgttgtctc gccgccgagg acgatgattc
      841 ggccgttcat cacgaacgtc gcgccagcga tgtgcgagcg cgcctgcgtg agcggcgtga
      901 gcgtcgtcca cacgccgggg ttggcggggt cccacatctg caccgcgctc tgcggcactt
      961 ccttcgcgtc ctgatgctgc tgcccgccga tcgcgtagat ccggccgttc agcaccaccg
     1021 cgcccaagtg attgcgcacg gtcggcagcg ccgcggcgac gtgccaagcg gtgtcgtgat
     1081 cgagatcgaa cgtccaatgc tcgctgcggt cttgtcgcgc cagatcggtc ccgccgaaga
     1141 aatgcagcac gcgaccgagc agtacgagcc cacccgcccc gcgcgcctgc ggcaatcccg
     1201 gcagcgtgct ccagctgttg gtgaccgtgt catagcgcat cacattcgtg cgcgcgaacg
     1261 tctgatttcc cccaccaccc ggcccgctat aaccgccggc gaagtagacg tagcggccgt
     1321 cgttgcagca accggaatgc gtcaggccca ccagcatcgg cgccaccggc gtccactgat
     1381 tcgtcgcggg atcgtataca tctgaacgcg cagccggcac gagcgtagtg ccgtcgagat
     1441 atccgccgaa cgtatacagc ttcccgttca ccaccgccgt ctgcgcttcg cttcgcggca
     1501 ccggtgacgg ctgcaccgtc gtccagctca ccttcgtgaa cgcgctcgga ttcgcctgcc
     1561 ccaccgtcgc ctgagcgacg ctgctgtacg ccgacgcacc ggcgttgttg atcgcgcgca
     1621 cgcgatagct gtagctcgca ccggccgtca cggccgtatc cgagtaactg ctcacgttgc
     1681 tcggcaacgt cgcgatgcgg atgaagctcg cgtcgcccgg cccctggcgc tccaactcga
     1741 agcccgcttc gttgttcgac gaatccggcc aggacagatc aacacgattc gtccccgcgg
     1801 cggcggtgag cgttaccgac gccgccgggg cgctcggctg cgcgtccacc ggatccagct
     1861 cgatcgccga gcaaatcgcg taatcggtga tgctctggaa cgtcaggttc agcgccccgc
     1921 cggtcacgct gaccggcacc gacttcacca gcgccttgtg atatcccacc tgcccgtaga
     1981 tatccaagtt cgacagcacc gtcgcgccct cggcctgcac gttgaacacg cgctgcccga
     2041 cggccgtctt cacgcgctcc atgaaataca gcttcagcgt gtaattcccg tcgtcggc
//
LOCUS       DATIMH010000148         7216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4078736, whole genome shotgun sequence.
ACCESSION   DATIMH010000148 DATIMH010000000
VERSION     DATIMH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4078736"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /gene="nrfD"
                     /locus_tag="VLI90_02480"
     CDS             complement(<1..1048)
                     /gene="nrfD"
                     /locus_tag="VLI90_02480"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HSV13098.1"
                     /translation="MAEDRKDAPYDAPWAQNLRHRPDPNRVSSLQTPTPGQFDRPQPL
                     DVRLPSDQATAQHRPLPVGAARDADPQSNAAPPSYYDIPMLKQPVWKWQIATYFYLGG
                     LSAGAYLLSRVADRAGGDKHRNVSRIGAYVAMATLVPCPPLLIWDLGDPKRFHHMLRV
                     WKPSTPMNLGTWSIVAYSGMAAYEVVRQYLTERENRVDAASLGAADRNKLLQVMNNGV
                     LLALHDAAGIPFSLMVAGYTGVLLSCTANPLWCKNPHLGPLFSASAVSTGAEAISLAL
                     DLLGDQPASQSALQNIDTAAHVAELACMAGFSRFAGEKADPLRRGLMKKHHLFSLGAI
                     VGAEVLKRIPVDNEL"
     gene            complement(1041..1970)
                     /locus_tag="VLI90_02485"
     CDS             complement(1041..1970)
                     /locus_tag="VLI90_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSV13099.1"
                     /translation="MTLQLPILNRGIGGALRDAAHDAVVNFPNAFPPVRLPVPQPDDS
                     RQLSQIMGFFTDTTLCIGCKACEVACKQWNQLPSDGFEFTANSYDNTKHLGATTWRHV
                     AFKEQIAGDGDGTDAVSRWLMMSDVCKHCDEAPCQHSCPTGAIIYNNFGDVYVQNDIC
                     NGCGYCVAACPFGVIGRADEQHDDGHAHKCTLCMDRQEEGMTPACAKVCPTSSIQFGP
                     LDQLRDAARQRVGDLKDRGNDRAYLYGCDSVGDYGPLNAFFLLEDHPNSYNLPVDPPQ
                     PFRGQKARYAASLGVGALLVGVAAALFGGARHG"
     gene            complement(1981..5355)
                     /gene="fdh"
                     /locus_tag="VLI90_02490"
     CDS             complement(1981..5355)
                     /gene="fdh"
                     /locus_tag="VLI90_02490"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_271413140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4810..4812),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HSV13100.1"
                     /translation="MFNLPLFGSNGNSHKRPPQPFSHHSIQKHARVRGATLTHSVCPY
                     CAVGCGTNIYTKGGEVIDIEGNPDSPINEGTLCPKGENIFQLHHNPHRVKNVLWRAPY
                     SDRWEVKPLKWAMDRIAQLVKQTRDEGYKEKNEDGKYTNYVLNMGCLGGATLDNEENY
                     LFKKLFGAGLGIVHIENQARIUHSASVPGLGASFGRGAATNYQQDLANSDCILIMGSN
                     MAEAHPVGFRWPMKARERGATLIHVDPRFSRTSACANKYVQIRAGTDIAFVGGLINYV
                     LTHDKWFKEYVLNFTNAATIIDEQFQDTEELGGIFSGYDHKARAYEAGKGRWAYKDSP
                     AAREHASGDRGEQQPEAQGTGGIGKSGAGDREAGSDMQHGIHGPGLMGGASTHTSKRG
                     GTSCADPERDPTLTHPSCVMNLLRRHFARYTPEMVSKICGCKPEEMEQVAELLCRNSG
                     RDRTSAVVYAVGWTQHSVGPQIIRAAGILQQLLGNIGRPGGMIMAMRGHCSIQGSTDI
                     PTLYDLLPGYLPQPTTEEHHGTIDGYVEYEGLRTGYWSKMKPMTVSLLKAYYGPAAAA
                     ENDFRFKWLPRIDGDYSQLPFFERMSRGEVKGYFVFGQNPAAGAPNAGLHRAGLRQLE
                     WLVVADWFETETATFWKNDPKGPKPAEVKTEVFFIPAASIAAKEGSFTNTQRLLQWHD
                     KAVDPEGDCRSDLSFAWNLGRRIKELYKGSTKPQDQAIQALTWDYGYDEHPHLPDGRP
                     SRLTDDPDAAKVLQEINGYFTNQTDEKTGKPKLLPGFSALKDDGTTACGCWIYSGVFP
                     SYDHNRARDRKLGNNPLQPDWAYAWPHNRRVQYNRASADPQGNPWSERKKLIWWDTEK
                     KKWVGFDEPDFEPDKSPDYRPPPGATGMDAIAGTHPFIMKPDGVSWLFSSAMKDGPFP
                     THYEPAESPVTNLLYPKQNDSPVVRYFEGPMNLIDHVPSQQFPIVACTFRVTEMYLSG
                     PMSRFNSWLNELMPEMFVEMSPELAAEKGIRNGEWCTVQSARGSIEAKALVTRRLRPL
                     QIDGKLVHQIGIPFHWGFAGESVGDIANDLVSITAEPNVSIQEDKAFGVDVEAGRTHS
                     SNQRPTKPFAPWPTVMPAPDTPASAQPEGQVHRGKL"
     gene            5866..6180
                     /locus_tag="VLI90_02495"
     CDS             5866..6180
                     /locus_tag="VLI90_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13101.1"
                     /translation="MAIQQRYCVNDPQRLAIGVCVMTRKAICDKCSTRYEGVNYSREG
                     LLLLQQQRAAAAQRAARAGAAGRSIATLLGTLLRSAMLYLVCLAYLTSADVVTSMLQR
                     GQ"
     gene            complement(6445..>7216)
                     /locus_tag="VLI90_02500"
     CDS             complement(6445..>7216)
                     /locus_tag="VLI90_02500"
                     /inference="COORDINATES: protein motif:HMM:NF044456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HSV13102.1"
                     /translation="PREPWNFGEPGTPFYDAILRSIQLRYRLLPYIYSQAGLVHLRGA
                     SFLRPVAFAFPGDSQAHDLKTQLLLGDGMMVSPVVTPMYYAPHSVPIEAAAKSREVYL
                     PQGRWIDFWTGETYGGNTTITADAPIARIPLHVRAGSIIPMGPAVQYASESPEAPIEL
                     RVYPGADGDYTFYEDAGEGWAYERGEYALVRMRWDDARRTLTIGARQGDFSGMIAKRQ
                     FKVILVSPGDGCGVEPAEHALDLSYEGRAVTVSLPAAR"
BASE COUNT         1232 a         2293 c         2272 g         1419 t
ORIGIN      
        1 ggagttcgtt gtccacgggg atgcgcttga ggacttcggc gccgacgatc gcgccgaggc
       61 tgaacaggtg atgctttttc atcagccctc ggcgcagcgg atcggctttc tcacccgcga
      121 agcgcgagaa gccggccatg cacgcgagtt cggcgacgtg ggccgcggta tcgatgttct
      181 gcagcgctga ttgcgatgcc ggttgatcgc cgagcagatc gagcgccagg ctgatcgcct
      241 cggcgccggt gctgacggcg gatgcgctga acaacggtcc gagatgcggg ttcttgcacc
      301 acagcggatt cgcggtgcag ctgagcagca cgccggtgta gccggcgacc atcagcgaga
      361 atggaatacc cgccgcgtcg tgcagcgcga gcagcacgcc attgttcatc acctgcagca
      421 gcttgtttcg atcggcggcg ccaaggctgg ccgcgtcgac gcgattttcg cgctcggtga
      481 gatattgccg cacgacttca taggcggcca tgccgctgta ggcgacgatg ctccacgtgc
      541 cgaggttcat cggcgtgctc ggcttccaca cgcgcagcat gtggtgaaaa cgcttgggat
      601 cgcccagatc ccagatcaac agcggcgggc acggcaccag cgtcgccatc gcaacgtacg
      661 cgccgatccg cgagacattg cggtgcttat ctccccccgc acggtcagca acacgcgaca
      721 ataaatatgc gccggccgac aaaccgccga ggtaaaaata cgtcgcgatc tgccacttcc
      781 acaccggctg cttgagcatc gggatatcgt aatacgacgg cggcgccgcg ttcgattgcg
      841 gatcagcgtc gcgcgccgcg cccaccggca gcgggcgatg ctgcgccgtc gcttgatccg
      901 agggcaaccg cacatccagc ggctgcgggc gatcgaactg ccccggcgtt ggtgtctgaa
      961 ggctcgatac gcggttgggg tcggggcgat gccgaaggtt ctgggcccag ggcgcatcgt
     1021 acggggcatc tttcctgtct tcagccatga cgtgcgcctc cgaacaaagc cgcggccaca
     1081 ccaaccagca acgcgccgac acccaagctg gcggcgtatc gcgccttctg cccgcgaaac
     1141 ggctgcggtg gatcgaccgg caggttgtaa ctgttgggat gatcctccag caggaagaac
     1201 gcgttcagcg gaccatagtc gccgacgcta tcgcagccgt agagatacgc gcgatcgttg
     1261 ccgcgatctt tcagatcgcc cacgcgttgt cgcgccgcgt cgcgcagttg atcgagcggc
     1321 ccaaactgga tcgacgacgt cgggcagacc ttcgcgcacg cgggggtcat gccttcttcc
     1381 tggcgatcca tgcacagcgt gcacttgtga gcgtggccgt catcgtgctg ctcatcggcg
     1441 cgaccgatca cgccaaaggg acacgcggcc acgcagtaac cgcagccgtt gcagatgtcg
     1501 ttctgcacgt acacgtcgcc aaagttgttg tagatgatcg cgccggtggg acaggaatgt
     1561 tggcacgggg cttcgtcgca atgcttgcag acgtcgctca tcatcaacca gcggctgacg
     1621 gcgtcggtgc cgtcgccgtc accagcgatc tgctctttga aggcgacgtg tcgccaagtc
     1681 gtcgcgccca ggtgcttggt gttgtcgtaa ctgttggcgg tgaattcgaa accgtcgctg
     1741 ggcaattggt tccactgctt gcacgcgacc tcgcacgcct tgcagccgat gcacagcgtg
     1801 gtgtcggtga agaagcccat gatctggctg agctgccgag aatcatccgg ctgcggcacc
     1861 ggcaggcgca cgggtggaaa ggcgttggga aagttgacga ccgcgtcgtg tgccgcgtcg
     1921 cgcagggctc cgccgatgcc gcgattgagg atgggcagtt gcaaagtcat tcgaccgcct
     1981 tcataacttc ccgcgatgga cctggccttc gggctgcgct gacgcgggtg tgtccggcgc
     2041 gggcatcacc gtcggccacg gcgcgaatgg ttttgtcggc cgctgattgc tcgaatgagt
     2101 ccggccggct tcgacgtcca caccgaaggc tttgtcttcc tgaatgctga cgttcggttc
     2161 ggctgtgatc gagaccaggt cattcgcgat atcgccaaca ctttcaccgg cgaaacccca
     2221 gtggaacggg atgccgatct ggtgcacgag ttttccgtcg atctgcaacg gccgcaggcg
     2281 gcgggtgacc agcgccttgg cttcgatcga gccgcgcgcc gattgcacgg tgcaccactc
     2341 accgttgcga atccccttct ccgccgccag ctctgggctc atctcgacga acatctcggg
     2401 catcaactca ttgagccagg agttgaaccg gctcatcggg ccgctcaaat acatctcggt
     2461 cacgcggaac gtgcaggcga cgatcgggaa ctgctggctg ggcacgtggt cgatcaggtt
     2521 catcggccct tcaaaatatc gcaccaccgg cgaatcgttc tgcttcggat acagcaggtt
     2581 cgtcaccggc gattcagccg gctcatagtg cgtggggaac gggccatcct tcatggcgga
     2641 cgagaatagc cagctcacac catccggctt catgatgaac ggatgcgtgc cggcaatcgc
     2701 gtccattccc gtcgcccccg gcggcgggcg atagtcgggt gatttgtccg gctcaaagtc
     2761 cggctcgtcg aaacccaccc actttttctt ttccgtgtcc caccagatca gcttcttgcg
     2821 ctctgaccaa gggttgccct ggggatcggc ggaggcacgg ttgtattgca cccgccgatt
     2881 gtgcggccac gcgtaagccc aatcgggctg gagcgggttg ttgccgagct tgcggtcgcg
     2941 cgcgcggttg tggtcgtagc tggggaagac gccggaatag atccaacaac cgcacgcggt
     3001 ggtgccgtcg tctttcagcg cggagaagcc cggcaatagt tttggcttgc ccgtcttctc
     3061 gtcggtttga ttggtgaagt agccgttgat ctcctgaagc accttggcgg cgtcggggtc
     3121 gtcggtcaac cggctcggcc gcccatccgg cagatgtggg tgctcatcgt acccgtagtc
     3181 ccaggtcagc gcttgaatcg cctggtcctg cggcttggtt gagcccttgt atagttcctt
     3241 gatccgccgg cccaggttcc atgcgaacga caaatcggag cggcaatcgc cttccggatc
     3301 aacggcctta tcgtgccact gcagcaggcg ctgggtgttg gtgaagctgc cttccttcgc
     3361 cgcgatgctc gccgcgggga tgaagaagac ttcggttttc acttccgcgg gcttaggtcc
     3421 cttcgggtcg ttcttccaga acgtcgcggt ttccgtttcg aaccagtcgg cgacgaccag
     3481 ccattccaac tgccgcagcc cggcgcgatg cagccccgca ttgggcgcgc cggcggccgg
     3541 gttttggccg aagacgaagt aaccttttac ttcgccgcgc gacatgcgtt cgaagaacgg
     3601 cagctggctg tagtcgccgt cgatgcgcgg cagccatttg aagcggaagt cgttttccgc
     3661 cgcggcagcc gggccgtaat acgccttgag caagctgacc gtcatcggct tcatcttcga
     3721 ccaatagccg gtgcgcaagc cttcatattc cacgtagccg tcgatcgtgc cgtgatgttc
     3781 ttcggttgtc ggttgcggca aatagccggg caacagatcg tacagcgtag ggatgtcggt
     3841 gctgccctgg atggagcagt gaccgcgcat cgccatgatc atcccgcccg gccgaccgat
     3901 gttgccgagc agctgctgca ggattcccgc cgcgcggatg atctgcggac caaccgaatg
     3961 ctgcgtccaa ccgacggcgt agacgaccgc gctggtgcga tcgcgcccgc tgttgcggca
     4021 aagcaactcg gcgacctgct ccatttcttc cggcttgcag ccgcagatct tcgaaaccat
     4081 ttccggcgta tagcgcgcga aatgccggcg caacaggttc atcacgcagc tgggatgtgt
     4141 gagcgtggga tcacgctctg gatcagcgca gctcgtcccg ccgcgtttgg aggtgtgcgt
     4201 gctcgcgccg cccatcaaac cggggccgtg gatgccgtgc tgcatgtcac tgccggcttc
     4261 tcgatcgcca gcgcccgact tgccgatgcc gcccgtgcct tgcgcttccg gctgttgctc
     4321 gccgcgatcg ccgctggcat gttcgcgggc cgccggtgaa tccttgtacg cccaccgccc
     4381 cttaccggct tcgtacgcgc gcgctttgtg gtcgtacccg ctgaagatcc cacccagctc
     4441 ttcggtgtcc tggaactgct cgtcgatgat cgtcgcggcg ttcgtgaagt tcaggacgta
     4501 ttccttgaac cacttgtcgt gggtcagcac gtaattgatc aacccgccga cgaacgcgat
     4561 gtcggtcccc gcgcggatct gcacatactt gttcgcgcac gcgctggtcc ggctgaagcg
     4621 cggatcgacg tggatcagcg tcgccccgcg ctcgcgcgcc ttcatgggcc agcgaaaacc
     4681 caccgggtgg gcctcggcca tgttcgagcc catgatcagg atgcagtcgc tgttggccag
     4741 gtcctgctga tagttagtgg ccgccccgcg gccaaaagag gcgcccagac cgggcaccga
     4801 agcgctatgt catatcctcg cctggttttc gatgtgcacg atccccaacc ccgcgccgaa
     4861 cagtttcttg aacaggtagt tctcttcgtt gtccagcgtc gccccgccga ggcagcccat
     4921 gttcagcacg tagttcgtgt acttcccgtc ttcgttcttc tctttgtacc cttcatcgcg
     4981 cgtctgcttg accagctgtg cgatgcgatc catcgcccac ttgagcggct tcacttccca
     5041 acgatcggaa tatggcgccc gccacagcac gttcttcacg cggtgcgggt tgtgatgcag
     5101 ctgaaaaatg ttctcgccct tcgggcagag cgtgccctcg ttgatcgggc tatcgggatt
     5161 cccctcgatg tcgatcacct caccgccctt ggtgtagatg ttagtcccgc agccaacggc
     5221 gcaatacgga cagacgctgt gcgtcagcgt agccccgcgc acccgggcat gtttctgtat
     5281 tgagtgatgg ctgaacggct gcggcgggcg cttgtgcgaa ttcccgttcg atccaaacag
     5341 cggcaggttg aacatcgcca atctccttct cggtcgatgc cccgaccata cggctgcccg
     5401 ccgaatttcg gcaatacgga ttttcccggc ccgcggtcac cttctcattt actgatcgct
     5461 ggcctgttaa atcgcgcggc tgttgacgtg ttccggcccc ctctcccacg agtacgctga
     5521 cgaaccgaca cttttgcagg aatggttggc gaccgaccac gccgggttcg gaggtactcc
     5581 gacgacccgg atccttcccg cgcgtccagc agaagaatct cgcttcgtcg cagtacctcc
     5641 gaacccggcg tgggtcggtc ccttgctacg tctgtaaaag tgtcgggccg tcggcacgag
     5701 cacgtgggac aggggaaagt cacgaacgcg ctttagctac tgccggatca gtaaccgaat
     5761 ggtcaagcgt gccgggatgc caggccgcgc attgcacgcc agccttgagc ccgccggcgt
     5821 tggcgcgata ggatgcgcgg agcgaagatc ggggttgagc cgcacatggc gatccaacag
     5881 cgttactgtg tcaacgatcc gcagcgcctg gcgatcggcg tttgcgtgat gacgcgcaag
     5941 gcaatttgcg ataaatgttc gacgcgctac gaaggcgtga actacagccg cgagggcctg
     6001 ctgctcttgc agcagcagcg agcggccgcg gcgcagcggg cggcacgcgc cggggcagcc
     6061 gggcgatcga tcgcgacact gctcggcacg ctgttgcggt ccgcgatgtt gtacctcgtc
     6121 tgcctggcgt acctgacgtc ggcggacgtg gtgacgagca tgttgcagcg gggccagtaa
     6181 ctcggggaac gtgacggatg acattggcgg gcggcctacg attctcgaac acgcctttta
     6241 gcggcggccc gaagggccga gtgttcgcgc gctggccacc agcgccagtc atggcgaaga
     6301 tcgaacacgt tgcagttaga ccgagagcgg cgaccaatgc cggtaagccg tagatgcgtg
     6361 cgcgccacgg cacgatcacg tcatcaggca atccattcga cggcgaacca gctgctgtcc
     6421 atcatcacca tgtggcggtc gatttcatcg cgcggcgggg agactgaccg tgaccgcccg
     6481 accttcatag gaaaggtcga gcgcatgttc cgcgggctca acgccgcagc cgtcgccggg
     6541 gctgacgagt atcaccttga attgtcgctt ggcgatcatc ccgctgaagt cgccttggcg
     6601 cgcgccgatg gtcagcgtcc gcctggcgtc atcccagcgc atgcgcacca gcgcatattc
     6661 gccgcgctcg taggcccatc cttcgccggc gtcttcgtag aaggtgtagt cgccgtcggc
     6721 gccggggtag acgcgcagct cgatcggcgc ttcgggcgat tccgacgcgt actgcaccgc
     6781 gggccccatc gggatgatgc tgcccgcccg cacgtgtagg gggatgcggg cgatcggcgc
     6841 gtcggcggtg atcgtcgtgt tgccgccgta agtctcaccg gtccagaaat cgatccacct
     6901 gccttgcggc aggtaaacct cgcgcgactt cgccgcggcc tcgatcggca ctgaatgcgg
     6961 cgcgtaatac atcggcgtca caaccggact gaccatcatg ccgtcgccca gcagcagctg
     7021 cgtcttcaga tcatgcgctt gcgaatcgcc gggaaatgca aacgcgaccg ggcgtagaaa
     7081 cgatgcgccg cgcaggtgca ccagcccggc ctgtgaatag atgtacggca gcagccgata
     7141 acgcaattgg atcgagcgca ggatcgcatc gtagaacggc gtgccgggct cgccgaaatt
     7201 ccacggttcg cgcggc
//
LOCUS       DATIMH010000149         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_902913, whole genome shotgun sequence.
ACCESSION   DATIMH010000149 DATIMH010000000
VERSION     DATIMH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902913"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VLI90_02505"
     CDS             <1..786
                     /locus_tag="VLI90_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13103.1"
                     /translation="IKAYLDGIRAQQKTALDKLAKFAAAVLDEAEKANIDKDARDRLE
                     SFANDDLRIALEGSDQLKPLQQRAMKLVSAFDQKAVGDAAARDKLYNTLIEQANKAWP
                     AMSKDFEATDGLDAVGASQKIESFKGKTVRLKAVQNRMGYDYSPSSGYDFAMTIDSVP
                     VAGKYEPAIRKAYNGITAKTGKDFSAEDCDIIATVEGMGPIVRIARTKGSVQTTGGEN
                     VGTITSEGNETVTGIRLKIIGLHVAPMAATSSGAVDENGNIAP"
     gene            complement(940..1431)
                     /locus_tag="VLI90_02510"
     CDS             complement(940..1431)
                     /locus_tag="VLI90_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13104.1"
                     /translation="MTSMTKLVAAAVLGAGLSLVGCEPNQSGTAGGYHSTSSEQNVGS
                     RSSHGLYGGTTDQTGSAGGGTYGNYSPATPATPYSNGTTPSNGTTPNSNNAIGTTGGS
                     GTAGGRYNGGVSGGTGVNQGGGATGTGTDAAGGYTTSGAGGNSGPIGGGTSQQNAGSG
                     SGR"
     gene            complement(1510..2046)
                     /locus_tag="VLI90_02515"
     CDS             complement(1510..2046)
                     /locus_tag="VLI90_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13105.1"
                     /translation="MDSNEKTDEEMTWERPTAALRQARYEANRRENISIFRMLLITVA
                     IASPISVVAIKWLRLEMPVWPVIVVPLIGALLPLVQLLLSAALERRLSRTQSPYLYRV
                     TKDGLELPQHGAFDRPFIRWNEVKWWRVRNAKRLTGFREIAIAVHRFGFRRLWLPSSE
                     MDAMIISSFEKYVGPPRS"
     gene            complement(2033..>2633)
                     /locus_tag="VLI90_02520"
     CDS             complement(2033..>2633)
                     /locus_tag="VLI90_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSV13106.1"
                     /translation="FATLDTKTRAWKLERGTEVLLSDTVGFVRDLPHNLVASFKATLE
                     EAVHADLLLHVLDVGHPHAQQQFESVHQVLEEIGVQGKPEVLLLNKIDTPEGEEGYPF
                     WRALMIGSIPISAKTGVGLAELNEAVYQHVRGQQVEATLEADVTNGRLLSFIESHTRV
                     QDRQFVDGRVRVRAVMGRQTLADLSRNHQVEVKHVDGLK"
BASE COUNT          519 a          820 c          799 g          495 t
ORIGIN      
        1 atcaaggcct atctcgacgg catccgcgcg cagcagaaga ccgcgctgga caagctggcg
       61 aagttcgccg ccgccgtgct ggacgaagcg gagaaagcga acatcgacaa ggacgcgcgc
      121 gatcgcctcg aaagcttcgc caatgatgac cttcgcatcg cgctggaagg ttccgatcag
      181 ctcaagccgc tgcagcagcg cgcgatgaag ctggtcagcg cgttcgatca aaaagccgtc
      241 ggcgacgccg cggcgcgcga caagctttac aacacgctca tcgagcaggc gaacaaggcc
      301 tggccggcga tgtccaagga ttttgaagcg accgatgggc tggacgcggt gggcgcgtcg
      361 cagaagatcg aatcgttcaa aggaaaaacc gttcgcctga aggccgtgca gaaccgcatg
      421 ggctacgact acagcccgag cagcggctac gactttgcga tgacgattga ttcggtgccg
      481 gtggcgggaa aatacgagcc agccatccgc aaggcgtaca acggcatcac cgctaagacg
      541 ggtaaggact tcagcgcaga ggattgcgac atcatcgcga cggttgaagg gatgggaccg
      601 atcgtccgta tcgcgcgcac gaaaggctcg gttcagacga ccggtggcga gaacgtcgga
      661 acgatcacgt ctgaaggcaa cgagaccgtc acggggattc gcctgaagat catcggcctg
      721 cacgttgcgc caatggcggc gacgtcgagc ggcgccgtcg atgagaacgg gaatattgcg
      781 ccttagtccg cattcacatt tgacgcggta aaagcccagg ccggaaggcg gcggatcgag
      841 cggcgcaaag cgcggcattg caagtcgatc gccggccttc cggcctgggc tattaatgtc
      901 gcggacagtt cctttacgcg ctggttatcc cgcgcgcgct caacggccgc tgccgctgcc
      961 ggcgttttgt tggctggtgc cgccaccgat cgggccgctg ttgccgccgg cgccgctggt
     1021 ggtgtaacca ccggccgcgt cggtgccggt gccagtggcg ccaccgcctt ggttcacgcc
     1081 tgtgccgccg gacacaccgc cgttgtatcg accacccgcc gttccggaac caccagtggt
     1141 gccaatggcg ttattggaat tgggcgtcgt gccgttgctc ggcgtggtgc cgttgctata
     1201 cggcgtggcc ggcgtggccg gtgagtagtt cccgtacgtc ccaccgccag cgctgccggt
     1261 ctggtcggtc gttccgccgt acaacccatg actgctgcgg ctgccgacgt tctgttccga
     1321 agatgtgctg tgataaccac cggccgtgcc gctctggttc ggctcgcagc cgacaagcga
     1381 caaaccggcg cccaacaccg cggcggccac caatttggtc atgctggtca tggattggtc
     1441 ctttcttact tgtagtgaac gatcaatctg caaagtgcgg accgatccaa tggttgagct
     1501 tggccttcct tatgaacgtg gaggcccaac gtacttctcg aagcttgaga tgatcatcgc
     1561 atccatctcc gagctcggaa gccataaacg ccgaaagccg aatcgatgca cggcgatagc
     1621 gatctcacga aacccggtga ggcgcttcgc gtttcgcaca cgccaccatt tcacttcatt
     1681 ccaccggata aaggggcgat caaaggcgcc atgctgtggt agttcaagcc cgtctttggt
     1741 gacgcgatat aaatacggcg actgtgtgcg cgataaccga cgctctagcg cggccgacaa
     1801 cagcaactgg accaaaggaa gaagcgcccc gataagcggc acgacgatca ctggccagac
     1861 cggcatttcc aggcgcagcc atttgattgc aacaacgcta atcggggacg cgatcgcgac
     1921 cgtaataaga agcattcgga atatcgagat gttctcgcgg cggttggcct catatctggc
     1981 ttggcgaaga gcggccgtcg gcctttccca cgtcatctct tcgtcggttt tctcatttga
     2041 gtccatctac gtgcttcacc tccacctgat gattgcgcga cagatccgcc aacgtttgcc
     2101 ggcccatcac cgctcgcacg cgcacgcgac cgtccacgaa ttggcgatcc tgcacgcgcg
     2161 tgtgcgattc gatgaacgac agcagccgcc cattcgttac gtccgcttcc agagtcgctt
     2221 cgacctgctg gccgcggacg tgctgataga ccgcttcgtt cagttccgcc aggccgacgc
     2281 cggttttcgc gctgatgggg atcgagccga tcatgagcgc gcgccagaac ggataaccct
     2341 cttcgccctc gggcgtgtcg atcttgttca gcaacaagac ctcgggcttg ccctgcacgc
     2401 cgatctcttc cagcacctgg tgcaccgact cgaactgctg ctgcgcatgc ggatggccca
     2461 catccagcac gtgcagcagc aaatccgcgt gcacggcttc ttcgagcgtg gccttgaagc
     2521 tggcgacgag attgtgcggc agatcgcgca cgaaaccgac cgtgtcgctc agcagcacct
     2581 cggtgccgcg ttccagcttc cacgcgcgcg ttttcgtatc gagcgtggcg aac
//
LOCUS       DATIMH010000150         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4658793, whole genome shotgun sequence.
ACCESSION   DATIMH010000150 DATIMH010000000
VERSION     DATIMH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4658793"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..769)
                     /locus_tag="VLI90_02525"
     CDS             complement(170..769)
                     /locus_tag="VLI90_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13107.1"
                     /translation="MTGKQLKSMGCSAAVLVAGIWMASLSGGCADTSAKRVETGGPES
                     IRTVDQIDDQDWSEAATKMTKSMLAQDNLFPPKSDGGKQVLEISRIINNTSQNVDTDL
                     LVKKIRVVLNQSGKVVTQTTDMRSRDVGNLTNFMDDKEKVIADYRLSGKMIENRAKAG
                     DIRQSTFYFQLSLTDKAGNDIWENEIPITKIGSHNAVGL"
     gene            complement(833..1267)
                     /locus_tag="VLI90_02530"
     CDS             complement(833..1267)
                     /locus_tag="VLI90_02530"
                     /inference="COORDINATES: protein motif:HMM:NF018889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HSV13108.1"
                     /translation="MMRRTNGLRLSTAALAAALAVAGGCKTENTYERDKPTYEANVIN
                     INKVETDPFLKDKAHVLEVRQADANGLLKVDVELVSDRLWSGSFDYKFEWFDDQGMPV
                     DSPMSSWTTRQVQPQERFSIDAIAPTPKCKDFRLKLQRSIRG"
     gene            complement(1310..>1946)
                     /locus_tag="VLI90_02535"
     CDS             complement(1310..>1946)
                     /locus_tag="VLI90_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13109.1"
                     /translation="VQIPLPLFIFSNQAPTVVLYFPVIKPRGDFAPYLRVDAGGQAYQ
                     TMLVSDIDAVMMQEFKNELPTVITRMIIGAATKAAIDAAAKQGLKNQNEIAQLGFDVV
                     STIYQVEMNQADLRVWRSLPKQFQVARFDTPADRTLSISVGDGSAPTTVTLAENPASR
                     VNVVYVKSVRAGLPPVIRQFAVTPGNPLQAPESAPATQPTTAPVNIAGAPQ"
BASE COUNT          325 a          625 c          565 g          431 t
ORIGIN      
        1 ccgcgagcga ctgcgttacc gctttggtaa tctgaggttc accgacttct gagccagatc
       61 cttcgactcc gcacgagtac gtgctgtgct caggacaaag tcagacaaaa aaagaagccc
      121 cgcatgtaca tgcggggctc cgattgcgct tcttccaatt cgatccagtt tacaacccaa
      181 ccgcgttgtg cgagccgatc ttagtgatcg ggatctcgtt ttcccaaatg tcattgccgg
      241 ccttgtcggt cagcgacaac tggaagtaga acgtggactg gcggatgtcg cccgccttgg
      301 cgcggttttc gatcatcttg cccgacaggc gatagtcggc aattaccttt tccttgtcat
      361 ccatgaagtt ggtcaggttg cccacgtcgc ggctgcgcat gtcggtcgtc tgtgtcacaa
      421 ctttgcccga ctggttcagc accacgcgga tcttcttgac cagcaggtcc gtatcgacgt
      481 tctgcgaggt gttgttgatg attcggctga tctccaggac ctgcttgccg ccatcggatt
      541 tgggcgggaa gaggttgtcc tgggcgagca tggacttcgt catctttgtc gccgcttccg
      601 accagtcctg gtcgtcgatc tgatcgacgg tgcggatcga ttccggcccg ccggtttcga
      661 cgcgcttggc ggaagtatcg gcgcacccgc cggacagcga ggccatccaa atccccgcca
      721 ccagcaccgc ggcggaacaa cccatcgact tcaattgttt gcccgtcatg tcagttacct
      781 ctcgttgatt taagtgttac gaatttacgc gttgcgcaat gctctctctt cgtcacccgc
      841 ggatgctgcg ctgcaatttc aaccgaaagt ccttgcactt gggcgtcggg gcaatcgcgt
      901 cgatcgaaaa ccgctcctgc ggctgcacct ggcgcgtcgt ccaactcgac atcggcgaat
      961 ccaccggcat gccctggtcg tcgaaccact cgaacttgta gtcgaagctg ccggaccaga
     1021 gccggtcgct caccagttcg acgtccacct tcagcagccc gttggcatcg gcctggcgga
     1081 cctccagcac gtgcgccttg tccttcagga acgggtccgt ctcaaccttg ttgatgttga
     1141 tgacgttcgc ctcatacgtc ggcttgtcgc gctcgtaggt gttctcggtc ttgcacccac
     1201 cggccaccgc cagcgcggca gccaacgccg cggtggacaa ccgcaaaccg ttcgttcgtc
     1261 gcatcatggc ctgttcactc ctttggttga cgtttgattt cgcacgcagc tattgcggcg
     1321 cgccggcgat gttcaccggc gcggtcgttg gctgcgtggc cggcgccgat tcgggcgcct
     1381 gcagcggatt gcccggcgtc accgcgaatt ggcggatgac cggcggcagc cccgcgcgca
     1441 ccgacttcac gtatacgacg ttcacgcgcg acgcgggatt ttccgccagc gtcaccgtcg
     1501 tcggcgcgct gccgtcgccg acgctgatgc tcagcgttcg atcggccggc gtgtcgaagc
     1561 gcgcgacctg gaattgcttg ggcaatgacc gccacacccg caggtccgcc tggttcatct
     1621 cgacctggta aatcgtcgag accacgtcga accccagctg cgcgatctcg ttctggttct
     1681 tcaagccctg cttggccgcg gcgtcgatgg ccgccttggt cgcggcgccg atgatcatgc
     1741 gcgtgatcac cgtgggcaac tcgttcttga attcctgcat catcacagcg tcgatgtcgc
     1801 tgacgagcat cgtctgatac gcctgcccgc cggcgtccac gcgaaggtac ggcgcgaaat
     1861 caccgcgcgg cttgatgacc gggaagtaca gcacgaccgt cggcgcctgg ttgctgaaga
     1921 tgaacagcgg cagcgggatc tgcacc
//
LOCUS       DATIMH010000151         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2925356, whole genome shotgun sequence.
ACCESSION   DATIMH010000151 DATIMH010000000
VERSION     DATIMH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2925356"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VLI90_02540"
     CDS             complement(<1..375)
                     /locus_tag="VLI90_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13110.1"
                     /translation="MAIDWKQLKKRVLVRGESAVASVGIAGAAILLAALAACGWWSLQ
                     AARQAQTQARTEEVRMVGTLLAKTAESMLANDELSPLRRLIMEASHNYRLSRCRIVLG
                     EDQVIADAEPSHINVRTLPVSWT"
     gene            complement(771..2306)
                     /locus_tag="VLI90_02545"
     CDS             complement(771..2306)
                     /locus_tag="VLI90_02545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV13111.1"
                     /translation="MNDEQQTGASQWPFIVHRLSLIVFLSIGVFLRWFHLGRESLWFD
                     EGYTAWVISLSPAQLIRAIRVDTAPPLYYLLLHGWAGLFGRSEFALRSLSALFSTASL
                     LLFYPLAFRFLSDRRLAAAALALFAVSEMQVAYAHEARLYTLVTLLAEIDLLLVLQAI
                     DRPPAWWRYLLMIVAWTISLYTNNMLAVYLAALGLMWLILPGPRPWRRRVIDLVIVAI
                     PAALLFLPWVPTLLAQSASLKGRFWTGPPDRRLIYRTLSVVCGWNEEALGWDWRRGVA
                     VAAWIVTGIVLIGLVRPATHRRALALCAFGLLPILVVYIQSKLSQPIFMERAFIATTL
                     VMPILLLLPFVPGRFHPPTWLMALFTAGWLVGSTLSLRLNYVGEHREQWRDACRAVDE
                     GAARNRLIVFVANEGEMLYDYYSRHSDYSPSPNLIGVPTSIFAKDPPQTMMRIHSEAD
                     LYGLRAALGHREFDEVVFVESHPWFSDADRRVEKLLNASLPLLGHQEFTLIHVYRYGA
                     ASR"
     gene            complement(2303..>2765)
                     /locus_tag="VLI90_02550"
     CDS             complement(2303..>2765)
                     /locus_tag="VLI90_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13112.1"
                     /translation="RAIARSVPPPYESNLAELATRAWRELLPGRVWALPKSHWAYELD
                     FGSRDWMPALLENIDLDPGMLADRNNAAAIEFSPDEDGRFQYFTERLLQMLLGSDFLL
                     VFPHCWTICTIHSHKQLWWTTSDAVVRAGLEAMAPTQATTSGEQRTMSDER"
BASE COUNT          543 a          884 c          901 g          437 t
ORIGIN      
        1 ggtccaagac acgggcagcg tgcggacgtt gatgtgactc ggttcggcgt cggcaatcac
       61 ctggtcttcg cccagcacaa tccggcagcg cgacaggcga taattgtgcg acgcctccat
      121 gatcaaccgc cgcagcggcg acagctcgtc attggccaac atcgactcgg cagtcttggc
      181 cagcagcgtg ccgaccatgc gcacctcttc ggtgcgcgcc tgcgtctgcg cctggcgggc
      241 cgcctgcagc gaccaccatc cgcacgccgc cagcgccgca agcaggatag cggccccggc
      301 gatgccgacc gatgccaccg cggattcacc acggaccagc acccgcttct tcagctgttt
      361 ccagtcgatt gccatgttcc acaccacaca aacgggttcg acacgcggtt acctgcgcac
      421 gacaggtcgc gcagagatac ccaccgtcat atcctcttcg gctgatatcg accgatcgtg
      481 aagccggata aatcgcacag atggctttcg cgcaacggcc gataatgcgc ggtaatcagc
      541 gcctattgga gggcaacctc aagcaggcgg tgcgcgctct ggtcccctcg cccggagttc
      601 cggcctgacg aatcgacact tttgcaggaa tggttgggga ccgaccacgc cgggttcgga
      661 gggcctgccc tccgaacccg gcgtgggtcg gtcccttgct aagtctgcaa aagtgtcgga
      721 ccgtcgggag taccgggagc gggggaaaga gcgtgccatc gccctggcgc ttatcgagac
      781 gcggcgccgt agcggtagac gtgaatgagc gtgaattcct gatggcccag cagcggcaga
      841 ctggcgttca gcagcttttc gacgcggcga tcggcatcgg aaaaccaggg atggctttcg
      901 acgaagacga cttcgtcgaa ctcgcggtgg cctaatgcgg cgcgaaggcc gtacaagtcg
      961 gcttcgctgt ggatgcgcat catcgtctgc ggcgggtcct tcgcgaagat gctcgtcggc
     1021 acgccgatca ggttgggcga cggcgaatag tcgctgtggc gcgagtagta gtcgtagagc
     1081 atctcgcctt cgttggcgac gaagacgatg aggcggttgc gggcggcgcc ttcgtcaacg
     1141 gcgcggcacg cgtcgcgcca ttgctcgcga tgttccccga cgtagttcag ccgcagcgac
     1201 agcgtgctgc cgacgagcca gccggcggtg aataatgcca tcaaccacgt gggcggatgg
     1261 aatcgcccgg gcacgaacgg caggagcagc aggatcggca tgacgagcgt cgtcgcgatg
     1321 aacgcgcgct ccatgaagat cggctgcgac agcttgctct ggatgtagac gacgaggatc
     1381 ggcagcaggc cgaacgcgca gagcgcgagc gcccgccggt gtgtcgcggg gcgcaccagg
     1441 cctataagca cgatgccggt gacgatccac gcggccacgg cgacgccgcg tcgccagtcc
     1501 cacccgagcg cttcttcgtt ccaaccacag acgaccgata acgtgcggta gatcaaccgc
     1561 cgatcgggcg ggccggtcca gaagcggcct ttgagcgacg ccgattgcgc gagcaatgtc
     1621 ggcacccacg gcaggaacag caacgccgcg gggatggcga cgatcaccag atcgatcacg
     1681 cgacggcgcc acgggcgcgg gccgggcagg atcagccaca tcaggcccag cgccgccagg
     1741 tacaccgcca gcatgttgtt cgtatacagg ctgatcgtcc acgcgacgat catcagcaga
     1801 taccgccacc acgccggcgg gcggtcaatc gcctgcagga ccagcagcag gtcgatctcg
     1861 gccagcagcg tgacgagcgt gtacaagcga gcctcgtgcg cgtacgcgac ctgcatttcc
     1921 gaaacggcaa acaacgccaa cgccgccgcg gcgagccggc gatcgctcag gaatcggaac
     1981 gcgagtgggt agaacagcag cagcgaagcg gttgagaaca gcgccgacag tgaccgcagg
     2041 gcgaattccg atcgcccgaa cagcccggcc cacccgtgca gcagcaggta atacaacggc
     2101 ggagccgtat cgacgcgaat cgcgcggatc aattgcgccg gcgacagcga gatcacccac
     2161 gcggtgtagc cctcgtcaaa ccagaggctc tcgcggccga ggtggaacca gcggaggaag
     2221 acgccgatcg aaaggaaaac gataagcgat aagcgatgaa cgatgaatgg ccattggctg
     2281 gcgccagtct gctgctcatc gttcatcgtt catcgctcat cgttcgttgc tcgccgctgg
     2341 tcgttgcttg tgttggcgcc atcgcttcca aacccgctcg aacgaccgcg tcgctggtcg
     2401 tccaccatag ctgcttatga ctgtgtatcg tgcagatcgt ccagcagtgc gggaagacca
     2461 gcagaaaatc gctgccgagc agcatctgga gcaggcgctc ggtgaaatat tggaaccggc
     2521 cgtcctcgtc tggcgagaat tcgatggccg ccgcgttatt gcggtcggcg agcatgccgg
     2581 ggtcgaggtc gatgttctcc agcagcgccg gcatccaatc gcggctgccg aagtccagct
     2641 cgtacgccca gtgacttttg ggcagcgccc acacacggcc cggcagcagc tcgcgccacg
     2701 cccgcgtggc cagctcggcg aggtttgact cgtacggcgg cgggactgag cgagcgattg
     2761 cgcgc
//
LOCUS       DATIMH010000152         4400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1193325, whole genome shotgun sequence.
ACCESSION   DATIMH010000152 DATIMH010000000
VERSION     DATIMH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193325"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..2052
                     /gene="lepA"
                     /locus_tag="VLI90_02555"
     CDS             265..2052
                     /gene="lepA"
                     /locus_tag="VLI90_02555"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSV13113.1"
                     /translation="MQIRNFSIIAHIDHGKSTLSDRLLMRAGAITEREFQNQMLDDMD
                     LERERGITIKASAVTVFHEHNGEKFMLNFIDTPGHVDFHYEVQKALQACEGAILVVDA
                     TQGVQAQTVANAYAAIEVGLEIIPVINKIDLPSADPVRVAEEIEQVLGFRAEECLLVS
                     AKTGQGIDELIEALCEKLPPPRGNPEAPPRALIFDSIYDDYRGVIAYVRVVDGALAKG
                     QKIRFMGTDKLYQITDLGKLMPRPKQVDRIGNSEVGFVVAAIKDLHDVRIGDTITDDQ
                     NRAAEQLAGYKPIQQMVYCDFYPSGDTQYDELRDAMDRLSLNDSSFSFEPESNDALGF
                     GFRCGFLGLLHMEIIQERLEREFHIQMIQTAPTVTYEVKKTDGTVFRIDSPSELPNPT
                     HVEEIREPFIMMNLILPADGVGALMKLCEDRRGTYRKTEYIGATRVMLQYELPLAEVI
                     YDFYDRLKSATKGYGTMDFDFLEFRHGDLVKLDILVNGDRVDALSVIVHREHAEARGR
                     KLLVRLKEEIDRHLFEIPLQAAIGGKIIARETIKSVGKNVTAKCYGGDVTRKRKLLEK
                     QKEGKKRMKRVGSVDIPQEAFMAILESGD"
     gene            complement(2088..3143)
                     /locus_tag="VLI90_02560"
     CDS             complement(2088..3143)
                     /locus_tag="VLI90_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13114.1"
                     /translation="MPTSPPIDLPTVTIPCYRCGYDVRAQPVNGRCPECAASVADSRR
                     LALIPRRPAWRDADPRWRRRILTGSWMLVLMPLMAVLQQFQWTERIPAPIFYDVQRGQ
                     SLDESCFAMVYLYFMFCIGLVLLFSKERNRQPARLDWTQRWGVTGSYGVLLLGIPAYA
                     FIMALVIIGIAALLQSMPLRYQPAVTPLLVKLGTGYVYYGPHPTLLAEASLACCSSIV
                     VLLACVPLFNALRSSGPRVPAAIVLAPLALSAVVQVGYVLAYALHLNPLPPDWVGNHF
                     FYFNPEALASWFGDPGRIFFHGNVIAEAAKWLACVGIAVWLTIAQISAICWRRRLVRT
                     ASKRNQSTGTQEALTSR"
     gene            3786..>4400
                     /locus_tag="VLI90_02565"
     CDS             3786..>4400
                     /locus_tag="VLI90_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13115.1"
                     /translation="MLLGATLCYSQTTSPAASEVRAVTGFGCSSPLEFETIEPNHIRV
                     TVPPGMSLDGWLFRLQGVAGKTVQIDFTSEQSLAKWSTLNPVYADGAMALDDPALYAA
                     APSSGATTTAARVKVPNAAGQRWHFITNTKLLNKTTFTMMQTFDVDSVWVANRVPCTP
                     GYNERFIRGLSNNPLAKVVEIGRSQEGRPLLMVRIDGDGADEAAL"
BASE COUNT          909 a         1367 c         1392 g          732 t
ORIGIN      
        1 aatccgggtc gtcgaagtac ctccgaaccc ggcgtgggtc ggttacttgc taagtctgca
       61 caagtgtcgg gccgtcggcc tggcgtgccg ggagagggga ccagagcgcg cggttcccac
      121 atgcgtttgc cttggtcgcg catcgccaaa ccgcccccgc tcttggggaa cccccacatc
      181 gcgcgtagaa tgacctgtct cgcgtttttt cgtaccgaca acaacaggac ttgccccttt
      241 taagcggggc tcgcgaagca agtaatgcag atccgcaact tttcgatcat tgcccacatc
      301 gaccacggca agagcacgct gtccgatcgc ctgctcatgc gagccggcgc catcaccgag
      361 cgcgaattcc agaaccagat gctcgacgac atggacctgg aacgcgaacg cggcatcaca
      421 atcaaggcgt ccgcggtcac cgtgtttcac gaacacaatg gcgagaagtt catgctgaac
      481 ttcatcgata cgccggggca cgtggacttc cattacgaag tgcagaaggc gctgcaggcg
      541 tgcgaaggag cgatcctcgt cgtcgatgcc acgcagggcg tgcaggcgca gacggtggcg
      601 aatgcttatg ccgccatcga agtggggctg gagatcattc cggtcatcaa caagatcgac
      661 ctgcccagcg ccgaccccgt gcgcgtcgcc gaggaaatcg aacaggtgct aggcttccgc
      721 gcggaagagt gcctcctggt ctcggccaag accgggcagg gcatcgacga actgatcgaa
      781 gcgctgtgcg aaaaacttcc gccgccgcgg gggaatcccg aagcgccccc gcgcgccctg
      841 atcttcgaca gcatctacga cgactatcgc ggggtcatcg cgtatgtgcg cgtcgtggac
      901 ggcgcgctgg ccaaggggca gaagatccgc ttcatgggca ccgacaagct gtatcagatc
      961 acggacctgg gcaagctgat gccgcggccg aagcaggtcg atcgcatcgg caacagcgaa
     1021 gtgggtttcg tcgtcgcggc catcaaggac ctgcacgatg tgcgcatcgg cgacacgatc
     1081 accgacgacc agaaccgcgc cgccgagcag ctggccggtt acaagccgat ccagcagatg
     1141 gtgtactgcg atttctatcc atcgggcgac acgcagtacg acgagctgcg cgatgcgatg
     1201 gatcgattgt cgctgaacga cagctcgttc agcttcgagc ccgagagcaa cgacgcgctc
     1261 ggcttcggtt tccgctgcgg ctttcttggc ctgttgcaca tggagatcat ccaggaacgg
     1321 ctggagcgcg agttccacat ccagatgatc cagacggcgc cgacggtgac gtacgaagtg
     1381 aagaagacgg atggcacggt cttccgcatc gacagcccca gcgagctgcc gaaccccacg
     1441 cacgtcgaag agattcgcga gccgttcatc atgatgaacc tgatcctgcc cgccgatggc
     1501 gtgggcgcgc tgatgaagct ctgcgaagac cgccgcggga cgtaccgcaa gaccgagtac
     1561 atcggcgcga cgcgcgtcat gctgcaatac gagctgccgc tggccgaggt gatctacgat
     1621 ttttacgatc gcctgaagag cgcgaccaag ggctacggca cgatggattt cgacttcctg
     1681 gaattccgcc acggcgacct ggtgaagctg gacatcctcg tcaacggtga ccgggtcgat
     1741 gcgctgagcg tgatcgtcca ccgggagcat gccgaagccc gcggccgcaa gctgctggtg
     1801 agactgaagg aagaaatcga ccgccacctg ttcgagattc ccctgcaagc cgccatcggc
     1861 ggcaagatca tcgcgaggga aaccatcaag agcgtcggca aaaacgtcac tgcaaaatgc
     1921 tacggcggcg acgtgacgcg caagcgcaag ctgctggaga agcaaaagga aggcaagaag
     1981 cgcatgaagc gcgtgggcag cgtggacatc ccgcaggaag cgttcatggc gatcctggag
     2041 agcggggact gagttgccca tacgtgtaga gatgcacgga attccagcta tcggcttgtc
     2101 agcgcctctt gtgttcctgt cgattggttg cgcttcgaag cagttcgaac gaggcgcctg
     2161 cgccaacaaa tcgcgctgat ctgcgcgatc gtcaaccaca cggcgatgcc cacgcacgcc
     2221 agccacttgg cggcttcggc tatgacgttg ccgtgaaaga aaatcctgcc gggatcgcca
     2281 aaccagctcg ccagcgcttc gggattgaaa tagaagaagt ggttacccac ccagtcgggc
     2341 ggtagcgggt tcagatgcaa agcgtaagcg agaacgtatc caacctgcac caccgcgctc
     2401 agcgccagcg gcgcgagaac gattgctgcc ggcacccgcg gtccgctgct gcgcagcgcg
     2461 ttgaacagcg gcacacacgc cagcagcacg acgattgatg aacagcaagc cagggaggct
     2521 tcggcaagta gagttggatg cggtccgtaa tacacgtagc cggtgcccag cttgaccaac
     2581 agcggcgtca ccgccggctg gtatcgcagc ggcatggact gcagcagcgc cgcgatgccg
     2641 ataatgacca gcgccatgat gaacgcgtac gccgggattc cgagcagcaa gacgccgtag
     2701 ctgccggtca caccccagcg ctgggtccag tcgagccggg cgggctggcg atttcgttcc
     2761 tttgagaaca gcaggaccag gccgatgcag aacatgaagt agagatagac cattgcgaag
     2821 caggactcgt cgagcgattg gccgcgctgg acatcataaa agatcggcgc gggaattcgt
     2881 tcagtccatt ggaactgctg gagcaccgcc atcagcggca tgagcacgag catccaggag
     2941 cccgtcaaga tgcgccgccg ccagcgcggg tcggcgtcgc gccaagccgg ccggcgcgga
     3001 atcagcgcaa gccgccggga gtccgctacg gacgccgcgc attccgggca acgcccgttc
     3061 accggctggg cacgcacgtc atagccacag cgatagcacg ggatcgtgac cgtgggcaga
     3121 tcgatcggcg gtgaggttgg catgatggag atctggtgcg cgcgcaaaca gatgcgccgt
     3181 ccgaccaaag tatcatctca ctgggtatgg gggagaattt ttagtggctt tgcgcgcgcc
     3241 ggacattcag caaccttcgt cgccgccaat cccgccacgc gtttagccgc cgcggcctcg
     3301 cggcgcgaac gtcgaaaagc gcagccagcg caaaatggcc caggccgcgg cgcctaaacc
     3361 gggcgcgttg cgctccctcg ccaccttcac cgcagaaacg ggtgtaagtg aactttgggg
     3421 tccggcgcta ctctggtccc ctcgcccggt actccgggaa agggttaggg tgagggtgat
     3481 ttggtctggc gataaccgct caacgttcga atcgccctca ccccgaccct ctcccggaga
     3541 cctgcggtca gcagtaccgg gcgagagggg acggaccaga atgtagccag aagtcatagt
     3601 cgtacccgca gaaacaggtt ctcacccccc cggcgcttct cttctccacg ttcccatgat
     3661 gaaataacgc cgccggcgga gcatggctgg ttttgatttc cttgacggcg gagcacccaa
     3721 tggcaattgc gcgcacgcgt cgatccccgt ggcattctcc attcgccgtc gcttgcgccg
     3781 gcctcttgtt gcttggcgcg acgctttgct attcacagac cacctcgccc gcggcgagcg
     3841 aggtgcgcgc ggtcaccggc ttcggctgca gctccccgct ggagttcgaa acgatcgaac
     3901 cgaaccacat ccgcgtgacc gtgccgccgg ggatgtcgct cgacggctgg ctctttcggc
     3961 tccaaggggt tgccggcaag accgtgcaga tcgatttcac gtcagagcaa agcctggcga
     4021 agtggtccac gctgaacccg gtgtatgccg atggcgcgat ggcgctggac gatccagcgc
     4081 tgtacgctgc cgcgccgtcg agcggcgcga cgaccaccgc ggcacgcgtg aaagtgccga
     4141 acgcggcggg gcaacgctgg cacttcatca ccaacacaaa gctgctgaac aagacgacgt
     4201 tcacgatgat gcagacgttc gacgtggatt cggtctgggt cgccaatcgc gtgccgtgca
     4261 cgccgggcta caacgagcgg ttcatccgcg gcctgtcgaa caacccgctg gccaaggtgg
     4321 tggaaatcgg ccgctcgcaa gagggtcgtc cgctgctgat ggtgcggatc gatggcgacg
     4381 gcgctgacga ggccgcgctg
//
LOCUS       DATIMH010000153         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2346758, whole genome shotgun sequence.
ACCESSION   DATIMH010000153 DATIMH010000000
VERSION     DATIMH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2346758"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..3348)
                     /locus_tag="VLI90_02570"
     CDS             complement(112..3348)
                     /locus_tag="VLI90_02570"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HSV13116.1"
                     /translation="MPEITNIKSRSVPPPSSARGNSPFAYAELDVSTNFSFLRGASHA
                     DELVYRAAELGHAAIAITDLNSLAGVVRAQQAAKTAGIKLCVGARLRFTDAPDVLVWA
                     MDRGGYANLCRLLTTGKRRAEKGSCTLHLQDLLAAPAGLLAAIVPQRIDPDQRETDPS
                     WIENAARELKGAFDDRLSIAISRLYGPTDSMRLRHAESIARAFGIPLLATNQVHYHEE
                     HRQSLQDVLTCIRHGCTIRDAGFRLFPNGERFIKPPEDMHRLFHDMPEALRRTVAITD
                     RCMFSLEELRYEYPDEIVPAGQTSASHLSALSWQGAAERYPQGVPEKVRRQIEYELKL
                     IEELKIEPYFLTVYDLVRFARSRGILCQGRGSAANSAVCYCLGVTSVDPNTSNLLFER
                     FVSAARGEPPDIDVDFEHERREEVIQYVYEKYGRERAGMTAEVISYRGRSAVRDVGKA
                     LGLGLDAVGQLAKRLDGWRSGAPMENEIREAGLDPADRSVRMTVALTSQLLGFPRHLS
                     QHVGGMVMTRGPLCELVPIENASMQDRTVIEWDKDDIDALGILKVDVLALGMLTALAK
                     GMKLVNQTHPDDPPIELHTIPQEDASVYDMICAADTVGVFQIESRAQMSMLPRLKPRT
                     FYDLVIEVAIVRPGPIQGDMVHPYLRRRSGAEAITYPTPEMEAVLGRTLGVPLFQEQA
                     MRLVMVAARFSADQADQLRRAMAAWRYTGHIDRFHQKVIDGMTANGYTREFAEQCFNQ
                     IKGFGEYGFPESHAASFALLVYASAWVKRYHPAAFCCAILNAQPMGFYAPAQLVRDAR
                     EHGVEVRGVDINHSDWDCTLEPNAAGSTASISMTGGKRTWGRDGPAVRLGLRMVKGFA
                     NAHAERIMQCRGAVGRFQSIEHFHETTALPVSAITKLSAADAFASVAQTRRLALWETL
                     ALPEEQQPLPVTRRKKMPDPQLPLMTMGEEIQADYTAAGLSLKGHPVQMLRAELTQRK
                     VVPAAQVWNRRHGTWIKVAGLVIIRQRPGTAKGIVFATLEDETGIVNLIIRPDVYERY
                     RPAARSASMVQADGYVERQGEVLHVMAMRLHDLSGWLGGYSLHSRDFH"
BASE COUNT          595 a         1231 c         1076 g          641 t
ORIGIN      
        1 cttgcggctt cgcagacgga caggccgcag tcactgttct gcgaaaccgc aagcgactgc
       61 cgtgcctttc gcgcagctca ttacgcctgc gctcaggatg acgcgcgcac gctaatgaaa
      121 atcccgcgag tgcaatgaat acccgcccag ccacccgctc aagtcgtgca gcctcatcgc
      181 catcacgtgc agcacctcgc cttgccgctc gacgtagccg tccgcctgca ccatcgacgc
      241 gctgcgcgcc gcggggcgat agcgctcgta cacatccggg cggatgatca ggttgacgat
      301 gcccgtctcg tcttccagcg tggcgaagac gatccccttg gctgtgcccg ggcgctggcg
      361 gatgatgacc aggccggcca ccttgatcca cgtgccgtgc cggcggttcc aaacctgggc
      421 ggcggggacc accttgcgct gggttaactc cgcccgcagc atctggaccg gatgcccctt
      481 gagcgacagc cccgcggcgg tgtagtcggc ttggatttct tcgcccatcg tcatcagcgg
      541 caattgcgga tccggcatct tttttcgccg cgtcaccggc aagggttgtt gctcttcggg
      601 cagcgccagc gtttcccaca gcgccagccg gcgcgtctgc gcgacagacg cgaacgcgtc
      661 ggcggcggac agcttggtga tcgccgacac cggcagcgca gtcgtctcgt gaaaatgttc
      721 gatcgactga aaccgcccga ccgccccccg gcattgcatg atgcgctcgg cgtgagcgtt
      781 ggcgaatccc ttcaccatcc gcaaccccaa ccgcaccgcc ggcccgtcgc ggccccaggt
      841 gcgcttgccg ccagtcatcg aaatgctcgc cgtcgatccc gcggcgttgg gttccaacgt
      901 gcaatcccaa tcgctgtgat tgatatcgac gccgcgcacc tccaccccgt gttcgcgcgc
      961 atcgcgcacc agctgggctg gcgcataaaa gcccatcggt tgcgcgttca agatcgcgca
     1021 gcagaacgcc gcggggtgat agcgcttgac ccacgcgctg gcataaacga gcagcgcgaa
     1081 actggcggca tgggattcgg gaaagccgta ctcgccgaac cccttgatct ggttgaagca
     1141 ctgctcggcg aattcgcgcg tgtacccgtt ggcggtcatg ccgtcgatca ccttctggtg
     1201 aaatcgatcg atgtgccccg tgtatcgcca cgccgccatc gcgcggcgca gctggtcggc
     1261 ctgatcggcg ctgaaccgcg ccgccaccat caccagccgc atcgcctgtt cctgaaacag
     1321 cggcaccccc agcgtgcggc ccagcaccgc ctccatctcc ggtgtgggat acgtgatcgc
     1381 ctccgcgccg ctgcgccgcc gcaggtatgg atgaaccatg tctccctgga tcggtccagg
     1441 ccgcacgatc gcgacttcga tcaccagatc gtaaaacgtc cgcggcttca accgcggcag
     1501 catcgacatc tgcgcccgcg attcgatctg aaacacgccg accgtgtcgg cggcgcagat
     1561 catgtcgtac acgctcgcgt cttcctgcgg gatcgtgtgc agctcgatcg gcggatcgtc
     1621 gggatgcgtt tggttgacca gcttcatccc cttggccagc gcggtgagca tccccagcgc
     1681 cagcacatcc accttcagga tgcccagcgc gtcgatgtcg tccttgtccc attcgatcac
     1741 cgtgcggtcc tgcatcgacg cattttcgat cggcaccaat tcgcacagcg ggccgcgggt
     1801 catcaccatc ccgccgacgt gctggctgag gtgtcggggg aaacccagca gttggctggt
     1861 gagcgcgacg gtcatgcgca ccgatcgatc cgccgggtcc agaccggctt cgcgaatctc
     1921 gttctccatc ggcgcgccgc tgcgccagcc gtccagtcgc ttggccagtt gaccgacggc
     1981 gtccagcccc agccccagcg ccttgcccac gtcgcgcacg gctgatcgac cgcgatagct
     2041 gatgacttca gccgtcatcc ccgcgcgctc gcggccgtat ttctcgtaga cgtattgaat
     2101 gacctcttcg cgccgctcgt gctcgaaatc aacgtcgata tccggcggtt ccccgcgggc
     2161 ggcggagacg aaacgctcga acagcaggtt gctggtgttc ggatcgacac tggtgacgcc
     2221 caggcaataa cagacggcgg aattcgccgc cgatccgcgg ccctggcaca aaatcccgcg
     2281 cgagcgcgcg aagcgcacca ggtcgtaaac cgtcaggaaa tacggctcga tcttgagctc
     2341 ttcgatcagc ttcagctcat actcgatctg ccggcggacc ttttccggca cgccctgtgg
     2401 atatcgctcg gcggcgcctt gccacgacaa cgccgacagg tgcgatgccg acgtctgccc
     2461 ggccggcacg atctcgtcgg gatattcgta gcgcaattct tccagcgaaa acatgcagcg
     2521 atcggtgatc gccaccgtgc gccgcagcgc ttcgggcatg tcgtgaaaca ggcggtgcat
     2581 gtcctccggt ggtttgatga agcgctcgcc gttgggaaac aggcggaacc ccgcgtcgcg
     2641 aatcgtgcag ccgtggcgga tgcaggtcag cacgtcctgc agcgattgcc gatgctcttc
     2701 gtgataatgc acttggttcg ttgccaatag tggaatgccg aaggcgcggg cgattgattc
     2761 ggcgtggcgc agccgcatgc tgtcggtcgg gccgtacaag cgcgagatgg cgatcgacaa
     2821 ccggtcgtca aatgcgccct tcaattctcg agcggcgttt tcgatccacg atggatcggt
     2881 ctcgcgctga tcgggatcga tccgctgcgg cacgatggcc gccagcaatc ccgccggcgc
     2941 cgccagcagg tcctgcaggt gaagcgtgca cgaacctttt tccgcccggc gtttgccggt
     3001 ggtcaacagc cggcacaggt tggcgtaccc gccgcggtcc atcgcccaca ccagcacatc
     3061 cggcgcatcg gtgaagcgca gccgcgcgcc gacgcacagt ttgatgccgg ccgtcttggc
     3121 ggcctgttgg gcgcgcacga cgccggcgag gctgtttaaa tccgtgatcg caatggccgc
     3181 gtgccccagt tcggcggcgc gataaaccag ttcatccgcg tgcgatgccc cgcgcaggaa
     3241 cgagaagttc gtgctgacgt ccagctccgc ataagcgaac ggcgaattcc cgcgcgccga
     3301 agacggcggg gggaccgagc ggcttttgat gttcgtgatt tccggcatga ctcactcgtc
     3361 gcttgctcgc tcgccgctcg cgctgcgtca ctcgaagatc ccgtgcaaaa accaccgccc
     3421 ggtctgggcc acgcggaaga tccagtagcg gccgccgccc gcgtccagca cgtcgaagta
     3481 atcgcgcacc ttccaccgcc cgttccacca ctgcccggtg atccgctccg gcccgcgcac
     3541 gtg
//
LOCUS       DATIMH010000154         4836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2925752, whole genome shotgun sequence.
ACCESSION   DATIMH010000154 DATIMH010000000
VERSION     DATIMH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2925752"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1036)
                     /locus_tag="VLI90_02575"
     CDS             complement(2..1036)
                     /locus_tag="VLI90_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV13117.1"
                     /translation="MKSSQTKTSLATACGLVGAAVAFRSIVRAKRRIDFDNRTVIITG
                     GSRGLGLCLARRFLDQGANVAVCARDEAELAEARKELNARGGASRVLAVQCDITSPPQ
                     MTGMIDGIIRHFGSLDVLVNNAGIIQVGPIEMMTIADFERAMQTHFYAPLIAIEAALP
                     QMRRQGGGRIVNITSIGGKVAVPHLVPYSASKFALVGLSESLRMELRRHNIYVTTVCP
                     GLMRTGSPRHAEFKGQHQKEHAWFATSDVLPIVTIDADRMAKRIINATQHGQAELITP
                     WIANLQAKVYGLMPGVMGEMLGCANDMLPRPGGIGRRSAKGYDSDRLQPELARQRNEA
                     AAQRNNEVAR"
     gene            complement(1065..2309)
                     /locus_tag="VLI90_02580"
     CDS             complement(1065..2309)
                     /locus_tag="VLI90_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HSV13118.1"
                     /translation="MRYTKPALATIAAIIIAGCQQDKPELQTAANNNNAAQAMAQLAS
                     AKEGTISNASQRQVEPFALFRGPMPTGVAVSRDNRVFVNYPRWGDPVAYSVTEVKNGQ
                     AVPFPDDRITKLNMDDPQHSLISVQSVMCDARNRLWILDTGSINFQPHLPNATKLIAV
                     DLNTNQVLKTISFPPDVALPTSYLNDMRFDLGRGSEGTAYLTDSSDSGPNGIVVVDIA
                     SGKSWRRLNHHPSTKAEPNFKPIVEGQPLMARPPTGPAAYLKMGSDGIALSPDGKTLY
                     YCALASHHLYSVSTDALADQNKSDNDVAAAVNDLGDRGFASDGLLCDASGKLYLTDFE
                     HNAIRTRDTNGSYGVLVSDARLIWPDSMAIGGDGYLYVTANQLNRQPRFHRGQDLRKQ
                     PYVLFRVKVDAQPSQPGANRAG"
     gene            complement(2341..3219)
                     /locus_tag="VLI90_02585"
     CDS             complement(2341..3219)
                     /locus_tag="VLI90_02585"
                     /inference="COORDINATES: protein motif:HMM:NF005214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV13119.1"
                     /translation="MPKSSNGNGATKTKPAQQQARRPGLESEMTPRPKAVKHEYKAAG
                     KLEGKAAIITGGDSGIGRAIAIDFAKEGADVAIIYLNEHEDAKETKKLVEREGRKCVL
                     ISGDIGDEQFCRESVEQSVKELGGLDILVNNAAEQHPQKQITQISAYQLERTFRTNIF
                     AQFFMVKAALPHMKEGAAIINTASVTAYRGSGGLIDYASTKGAIVAFTRSLAENLAEK
                     KIRVNSVAPGPIWTPLIPSTFPKEKVEKFGADVPMKRPGEPAEVAPCYVFLASQDSSY
                     MTGQVLHPNGGEVVNA"
     gene            3830..>4836
                     /locus_tag="VLI90_02590"
     CDS             3830..>4836
                     /locus_tag="VLI90_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13120.1"
                     /translation="MATRKKATKAATTPRAHHAGHAKTPAAIDPPQSKQYPRPMLQRP
                     QWVNLNGQWDFAIDKAAELSHPRQVQWDRQITVPYSPETPASGVGDTGFYNAVWYRRT
                     FAVPDLRDGQRVLLHFGAVDYEAEVWVNDQLATKHEGGYTPFKADITDLLRGDGEQTL
                     VVRAIDDPQDLAKPRGKQDWLLDAHSIWYPRTTGIWQTVWLEVVPATHVEYVRWTPRF
                     EQWQIQLDARIVGPWRDGTRLRVRLSSRGQTLVDDTYAVGADKHVSRKILLPDPGIDD
                     ARNALFWWPHAPNLIDAELTLIGPDGQTIDVVTSYTAMRGVAILGDRFFLNGRPYKLQ
                     LV"
BASE COUNT          798 a         1510 c         1539 g          989 t
ORIGIN      
        1 tctagcgcgc gacttcgttg ttccgctgcg ccgccgcttc gttgcgctgc cttgccagtt
       61 ccggttgtag tcgatcgctg tcgtatccct tggcgctgcg ccgaccgatc ccgcctgggc
      121 gcgggagcat atcgttcgcg cagcccagca tctcgcccat cacgccgggc atcaggccat
      181 acaccttcgc ctgcaggttg gcgatccatg gcgtgatcag ttccgcctgc ccgtgttgcg
      241 ttgcgttgat gatccgcttg gccattcggt cggcgtcgat cgtcacgatc ggcagcacgt
      301 ccgacgtcgc gaaccacgcg tgctcctttt ggtgttgtcc cttgaactct gcgtgccgtg
      361 ggctgccggt gcgcatcagg ccggggcaga ccgtggtcac gtaaatgttg tgccggcgca
      421 gttccatccg cagcgattcg ctcaggccga ccagtgcgaa cttgctggcg ctatacggca
      481 ccaggtgcgg caccgcgacc tttccgccga tcgaggtgat gttgacgatg cgcccgccac
      541 cctggcggcg catctgcggc aacgccgcct cgatcgcgat cagcggggcg taaaaatgcg
      601 tctgcatcgc gcgctcgaag tcggcgatcg tcatcatttc gatcggtccc acctggatga
      661 ttcccgcgtt gttgaccagc acatcgagcg agccgaaatg ccggatgatg ccgtcgatca
      721 tcccggtcat ttgcggcggc gaggtgatgt cgcattgcac cgcgagcacg cgactcgccc
      781 caccgcgggc gttcaactct tttctcgctt cggccagctc cgcttcgtca cgggcgcaga
      841 cggcgacgtt ggcgccctgg tccagaaagc gccgggcgag gcacaggccc agcccgcgtg
      901 agccgccggt gatgatgacg gtgcggttgt caaagtcgat gcggcgtttg gcgcggacga
      961 tgctgcgaaa ggcgacggca gcgccgacaa ggccacaggc ggtcgcgagg ctcgttttgg
     1021 tttgactgga tttcatagag tcgctctgct tgctgcgcta attcttagcc ggctctgttc
     1081 gcgcccggtt gcgacggctg cgcatccacc ttcaccctga acaacacata cggctgcttg
     1141 cgcaagtcct ggccccggtg gaagcggggc tggcgattca gctgattcgc cgtgacgtac
     1201 aggtacccgt cgccaccgat ggccatcgaa tcaggccaga tcaagcgggc gtcgctgacc
     1261 agcacgccgt aggaaccgtt ggtgtcacgc gtgcggatcg cgttgtgctc gaaatcggtc
     1321 aggtacaact tgccggaggc gtcgcaaagc agcccgtcgc tggcgaagcc gcgatcgccg
     1381 aggtcgttca ccgcggcggc aacgtcgttg tcgctcttgt tttgatcggc cagcgcatcg
     1441 gtgctgacgc tgtagagatg gtggctggcc agcgcgcagt agtagagcgt tttgccgtcg
     1501 gggctgagcg caatgccgtc gctgcccatc ttcaagtacg ccgcggggcc cgtgggtggg
     1561 cgggccatca gcggctggcc ttcgacgatc ggtttgaagt ttggctccgc cttggtggat
     1621 ggatgatggt tcaaccgccg ccagcttttg ccgctggcga tgtcaacgac gacgatgccg
     1681 ttgggaccac tgtccgacga atcggtcaga tacgcggtgc cttcgctgcc tcggcccaaa
     1741 tcgaatcgca tgtcattcag gtaagacgtc ggcagcgcca cgtccggcgg gaacgagatc
     1801 gtcttcagga cctggttcgt gtttagatcg accgcgatca gcttcgtcgc gttcggcagg
     1861 tgcggttgaa agttgatgct gccggtgtcg agaatccaca agcgattccg cgcatcgcac
     1921 atcacgctct gcacgctgat cagcgaatgc tgcgggtcgt ccatgttcag cttcgtgatg
     1981 cgatcgtcgg ggaacggaac ggcttggccg ttcttgacct cggtcacgct gtacgcgacg
     2041 gggtcgcccc agcgcggata gttgacgaag acacggttgt cgcgcgaaac ggccacgccc
     2101 gtgggcatcg gcccgcggaa aagcgcgaac ggctcgacct gccgctgcga tgcgttgctg
     2161 atcgtgcctt ccttggcgct ggccagctgc gccatggctt gcgcggcatt gttgttgttg
     2221 gcggccgtct ggagttccgg tttgtcctgc tggcaaccgg cgatgatgat cgcggcgatc
     2281 gtcgctaacg ctggctttgt atatctcatc ggcagcctcg attgtttgaa gggcgagacc
     2341 tcatgcgttg acgacttccc cgccgttggg atgcagcact tggccggtca tgtacgatga
     2401 atcctgcgac gcgaggaaga cgtagcacgg cgcgacttca gccggttcac ccgggcgttt
     2461 catcgggacg tccgcgccga acttttccac cttctccttg gggaacgtcg atggaatgag
     2521 cggcgtccag atcggtcccg gcgcgacact gttcacgcgg atctttttct ccgccaagtt
     2581 ttccgccagg ctgcgcgtga acgcgacgat cgcgcccttg gtgctggcgt aatcgatcaa
     2641 cccaccgctg ccgcggtagg cggtcacgct cgcggtgttg atgatcgcgg ccccttcctt
     2701 catgtgcggc agggccgcct tcaccatgaa gaactgcgcg aagatgttcg tgcggaacgt
     2761 gcgctccagt tgatacgcac tgatctgcgt gatctgtttc tgcgggtgct gctcggcggc
     2821 gttgttgacc aggatgtcga gcccgcccag ttccttcacc gattgttcga ctgactcccg
     2881 gcagaattgt tcatcgccga tgtcgccgct gatcagcacg cactttcggc cttcgcgctc
     2941 gaccagcttc ttcgtctcct tcgcatcttc atgttcgttc agataaatga tcgcgacgtc
     3001 ggccccttcc ttcgcgaagt cgatggcgat ggcccggccg atgccgctgt ctccgccggt
     3061 gatgatggcg gctttgcctt cgagcttgcc ggcggctttg tattcgtgct tcaccgcctt
     3121 gggccgcggg gtcatctccg attccagccc cggccgtcga gcctgctgtt gggcgggttt
     3181 ggttttcgtc gcaccgtttc cgttagaact ttttggcatg gtgttcctcc gtgggacact
     3241 ttgggtaggc gcgaaggcgt gcaagcaatg tgccatcgac ggcgaggcga cttcaggttt
     3301 cgtggaccac gacggtgaag ctatgtgcgc aatcgccaag aagagcgcgt tgaacgatcg
     3361 ctcaacgacc atcgaatagt cgggaagaaa atgcgcagac gcagggggct ctgccccccg
     3421 caccccccgg agtttggcgc tttggcagtc accggcggga ggtgggagat gacggccgag
     3481 gcgcaatagg cgaagaccgc gcaatctcga agattgcgcg gtctcccgcc gtacgactga
     3541 ccggcgggcg ctcgggtcgc actcctgcgt tgccctatcc tcccgccggc aacgccgagt
     3601 ctagcgatgc accaatcatg ttcgcaatcg catttccgcc ggtgactgcc caagcactaa
     3661 actccggggg gtgcgggggg cagagccccc tgcgtctgcg catttctttt ttgctctatt
     3721 caacgcacgc aatcctcaat cgcccattcc ttcgcaacca gaaacccggt tcactcatca
     3781 cttgcagtcc gctacattcc ccgctttgct actgggatgg agctgaacca tggcgacacg
     3841 aaagaaggcg accaaagcgg cgacgacgcc gcgggcgcat catgcaggtc acgcaaagac
     3901 accggctgcg atcgatccgc cgcaatccaa gcaatacccc cggccgatgc tgcagcggcc
     3961 gcagtgggtc aatctgaacg ggcagtggga tttcgccatc gacaaagcgg cggaactgtc
     4021 gcacccgcgg caggtgcagt gggatcggca aatcacggtg ccatactcgc cggaaacgcc
     4081 ggccagcggt gtcggtgaca ccgggttcta caacgcggtg tggtatcgac ggacgtttgc
     4141 cgtgcccgat cttcgcgatg gccagcgcgt gctgctgcac tttggcgcgg tggactacga
     4201 agccgaagtg tgggtgaacg atcagctggc gacaaaacac gaaggcggct acacgccgtt
     4261 caaggcggac attacggatt tactgagggg tgatggggag cagacgctgg tcgtgcgcgc
     4321 gatagacgac ccgcaggatc tggccaagcc ccgcggcaag caggattggc tgctcgacgc
     4381 ccactcaatc tggtatccgc gcacgaccgg catctggcag accgtctggc tggaagtggt
     4441 gccggcgacg cacgtggaat atgtgcgctg gacgccgcgg tttgaacagt ggcagatcca
     4501 gctggacgcg cggattgtcg gcccgtggcg cgacggcact cgcctgcgcg tgcggctttc
     4561 atcgcgcgga cagacgctgg tggacgacac ctacgccgtc ggcgccgaca aacatgtctc
     4621 gcgaaaaatc ttgctgcccg atccgggcat cgacgacgcc cgcaacgcgc tgttctggtg
     4681 gccgcacgcg cccaacctga tcgacgcgga gctgacgctg atcggtcccg atgggcaaac
     4741 gatcgacgtc gtcaccagct acaccgcgat gcgcggcgtg gcgatcctcg gcgatcgctt
     4801 cttcctcaat ggccggccgt acaagcttca gctcgt
//
LOCUS       DATIMH010000155         6386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3502966, whole genome shotgun sequence.
ACCESSION   DATIMH010000155 DATIMH010000000
VERSION     DATIMH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3502966"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VLI90_02595"
     CDS             complement(<1..1227)
                     /locus_tag="VLI90_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13121.1"
                     /translation="MLKTSSRSAYRSVWHACEALERRRLLAAVAWDGGGDGTNWSDAN
                     NWSSNAVPGAADDVTIDVPGAVTVLVRGAQPSVQSLVNRETLWLRGDNTFGNAAASIS
                     GNVTNDGTIKLESIDSTWSDTLSVGGVLSNSSTGVVQMNAGTGGQRFITGNIDNSGAI
                     STTSAFSGGAAGKTFNQMGGSVTASGGGSFAWASGTMNFSGGAFSGAVYVNGGSLSVA
                     STAGAQTILVEGASTLLSNDSTAATVWVQGNNMVGGATLAIPGNATNNGTIRMESIDS
                     SWSETLNIGGVLSNSASGVIQVNAGTGGTRAINGNINNSGTINTAVAVTAGASGKTFN
                     QIGGTVTASGTGSFVWSSGTMNFSGGSFVGSVYLNGGSLSVASTAGAQTILVEGSSTL
                     LSNDSTAATVWVQGNSG"
     gene            complement(2312..3016)
                     /locus_tag="VLI90_02600"
     CDS             complement(2312..3016)
                     /locus_tag="VLI90_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HSV13122.1"
                     /translation="MSSLHICRRVVSSCFFSLMVAVAISAPGVAVFGDEPHGAAVAGV
                     SPQAALEKLKAGNKRFVEQKLADKDLAQRRTATANGQQPYAIILACADSRVAPELVFD
                     ETIGDLFVIRVAGNVVDPDVLGSVEYAVEHLHARLIVVLGHEKCGAVTAALSREGLHG
                     NLDELIHKVAPGDNLPDDKSRALPEAIKHNALHQAQEMVNQSEPIKKVVDEHDVQIEA
                     AVYSLDSGEVKWLENK"
     gene            complement(3673..5103)
                     /gene="atpD"
                     /locus_tag="VLI90_02605"
     CDS             complement(3673..5103)
                     /gene="atpD"
                     /locus_tag="VLI90_02605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSV13123.1"
                     /translation="MPATGKVTQVIGSTFDIEYPEDQLPKIYNAVTINAKNDGQADIR
                     LTGEVQQHLGGGKVRAVALGSTDGLRRGMDAVDTGGPVTVPVGKETLGRVFNLLGEPI
                     DNRGPVVTESRRGIHREPPAFVDLSPKAEVFETGIKVIDLLTPFLRGGKSGLFGGAGL
                     GKTVVIQELIARIAQQHGGYSVFAGVGERTREGTDLWLEMQEAEIGTTGKHVIDQTVM
                     VFGQMNEPPGARLRVALSALTMAEYFRDETGADTLLFIDNIFRFTQAGSEVSALLGRM
                     PSAVGYQPTLATEMGELQERITSTKNGAITSVQAVYVPADDPTDPAPANAFAHLDAFI
                     YLERSIASKGIYPAVDPLASASRALSADIVGEDHARIARKVQTMLQRYRDLQDIIAIL
                     GVDELSEEDKLIVSRARKIERFFSQPFFVAEQFTGKKGIYTKREETLKSFDAICNGEA
                     DDLPEQAFLYVGGLDDVRARAKEMAK"
     gene            5366..5674
                     /locus_tag="VLI90_02610"
     CDS             5366..5674
                     /locus_tag="VLI90_02610"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HSV13124.1"
                     /translation="MRVIKRLPLLEFGHQRPAAVKPLDAWYRATLAADWRNFAQVKAT
                     FGSTDQAKVASGQTVCIFGIGGNKFRLIAFVSYEKSKVYVLRILTHKEYDSNRWRSEL
                     "
     gene            5671..6099
                     /locus_tag="VLI90_02615"
     CDS             5671..6099
                     /locus_tag="VLI90_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13125.1"
                     /translation="MKSLTKPNRHDAAGDTYLDLVRHLPLRPIRSGKDYAEAAALLDR
                     LALLDDKLDAGQRDYLETLEMLINAYDDAHARVVPDGRTPLERLKYLMQQNAMTSSAL
                     GDLLGNRPLASMILRGKRGLSKAHIRKLADRFTVDAGYFL"
     gene            complement(6108..>6386)
                     /locus_tag="VLI90_02620"
     CDS             complement(6108..>6386)
                     /locus_tag="VLI90_02620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HSV13126.1"
                     /translation="LAEVLKSATPAAPTWVMSTQGAATPIAKLLQSPSLPAAITIFIG
                     PEGGWTDGELDQFTTAGATAVALTATILRIETAAVTAAAVVACWKPSH"
BASE COUNT         1150 a         2130 c         1883 g         1223 t
ORIGIN      
        1 gccgctgttc ccctgcaccc acaccgtcgc cgccgtcgag tcgttgctca acaacgtcga
       61 tgatccttcc accagaatcg tctgcgcgcc cgccgtcgaa gcgacgctca gcgacccgcc
      121 gttgagatag accgatccaa caaacgatcc cccgctgaag ttcatcgtcc cgctggacca
      181 gacgaaactg cccgtaccgc tggcggtgac ggttccgcca atctggttga acgtcttccc
      241 gctcgcgccc gccgtcaccg caacagccgt gttgatcgtc ccgctgttat tgatgttgcc
      301 gttgatcgcg cgcgtcccac ccgtgcccgc gttcacctga atcacaccgc tcgccgaatt
      361 gctcagcacc ccgccgatat tcagcgtctc actccacgaa ctgtcgatcg actccatgcg
      421 gatcgtgccg ttattggtgg cgttcccggg gatcgccagc gtcgccccgc ccaccatatt
      481 gtttccctgc acccacaccg tcgccgccgt ggaatcgttg ctcagcagcg tcgaagcgcc
      541 ttcgaccagg atcgtctgcg cgcccgccgt cgaagcaacg ctcaacgacc cgccgttgac
      601 gtacaccgct ccgctgaacg cgcccccgct gaagttcatc gtcccgctcg cccaggcaaa
      661 gctcccgccg ccgctggcgg tcacgctgcc gcccatctgg ttgaacgtct tcccagccgc
      721 gccgcccgag aacgcgctcg tcgtgctgat cgcgccgctg ttatcgatgt tccccgtgat
      781 gaaccgctgc ccgccggtcc ccgcgttcat ctgaacaacg cccgtggacg aattgctcag
      841 caccccgccc acgctcagcg tgtcactcca ggtactatca atcgactcca gcttgatcgt
      901 cccatcattg gtgacattgc cggagatgct cgccgccgcg ttgccaaacg tattgtcccc
      961 gcgcaaccac agcgtctctc gattgaccaa actctgcacg ctcggctgcg ctccgcgcac
     1021 cagcaccgtc accgcgcccg gcacgtcgat cgtcacatca tccgccgccc ccggcacggc
     1081 gttgctactc cagttgttcg catccgacca gttggtccca tccccgccgc catcccacgc
     1141 cactgccgcc agcaatcgcc ggcgttccag cgcctcgcaa gcgtgccaga cactccgata
     1201 agctgatcga gacgacgtct tcaacacacg aactggccga tgaatattct gctgcacaaa
     1261 gctcccttcc cgcgcacgcg ccgagataga aatgatcaac cgcgagcccg ccgaaaggcg
     1321 gaccacccaa ttcagctgta aacgacggct taagcagacg tgaatgaacc gccccgttga
     1381 ggaccgagag atatcccacc agccaggcga ttcgcgctgg cgcgccaaca aatgagcaaa
     1441 cgcccccgct cacgcgcgcc acggcggcgg ctctttgaag agcgccgcgc caattatgct
     1501 aaaccttgcg ggatcggcat tcaatcagta aatctgctgg cttttgctgc caaaaccccg
     1561 ccatcgccca accatggccc aggagtggca ccccggacgt aatagttcga ttcctaccag
     1621 cgctccgccc tcacgacccg acactttggg gaacaaccag cggaacccca gtttcccatc
     1681 gcccgatgcg aggtgaatcg atgttcgcca cgccgggttc ggaggtactc cgacgacccg
     1741 gattctgcgg cgcgcgagaa gaatccgggt cgtcggagta cctccgaacc cggcgtggat
     1801 cggttcctca ccacttctgc aagagtgtcg gaccgtcagg cgctccgccc gggtgacgtg
     1861 tggctcgaac tgccgggcta tattcaatcg tccggatgtg ctaacgggga tcttggcggc
     1921 gcggttgggt cgacgttgta gtgacgctcg tacgggtcat tcccaagcgc cttctccgtc
     1981 gcgaaatcca atttgtcccg cgcgagcttg tcctccgccg tcttgttcgg cagaagcgca
     2041 tcgcacccgg cgagaacaaa catggctagc cacggcaata agagacgccg gcctcgcatc
     2101 gttggcatca tggtcatcgg ccgagaagta agtgagtcat ccatccggcg ccggagcccc
     2161 gcatgtacat ttggagcttc gaaggtccat gaacgcctgc gctatcgaat cgctccatca
     2221 aatctcatca agatccgtcg acttcggcga atacgcctgc gctcatgatg gccgcgctac
     2281 ggtcgcttcc cgcgtacgct cgcttcccgc gctacttgtt ctccagccac ttcacctcgc
     2341 cgctgtccag gctgtacacc gcggcttcaa tctgcacatc gtgttcatcg accaccttct
     2401 taatcggctc gctctggttc accatctcct gggcctggtg aagcgcattg tgcttgatcg
     2461 cctcgggcag cgcccgcgat ttgtcatcgg gcagattgtc gccgggggcg accttgtgga
     2521 tcagctcatc gaggttgccg tgcaggccct ccctggaaag ggcggccgtg actgcgccgc
     2581 acttttcgtg gccgagcacc acgatcagcc gggcgtgcag atgctcgacc gcgtattcga
     2641 ccgatccgag cacgtccggg tccaccacgt tgccggccac gcggatgacg aacagatcgc
     2701 cgatcgtttc gtcgaagacc agctccggcg cgacgcgcga atcggcgcag gccaggatga
     2761 tcgcatacgg ctgctgcccg ttggccgtcg cggtgcgacg ctgggccagg tccttgtccg
     2821 ccagcttctg ttcgacaaac cgcttgttgc cggccttgag tttctccagc gccgcttgcg
     2881 gcgacacgcc ggcgacggca gcgccgtgcg gctcgtcacc aaacaccgca acgccaggcg
     2941 cgctgatcgc gacggcgacc atcagcgaga agaaacagga cgacacaacc cgccggcaaa
     3001 tgtgcagcga tgacatgagc aagcctttct gcccggaatg aggaggggga tgaacgcgcc
     3061 ggaatagtct cgcgcgggca gggcagcgtc aaatgctctc gagtgcatca gtgcaaggtg
     3121 gatgcggcgc tccgtccgac tagcgccgca atactccgca cagaagcgat gcgcgtttag
     3181 ccgccgcggc ctcgcggcgc aaacttcgag ttgcgacgct gttctaatcc gcgccgcgag
     3241 gccgcggcgg ctaaacgatg gcgtttcttg ggctcttcgt tcgatgttct tgatggcggt
     3301 cagattctac cgtcggaggc gacggtacgc gtgaattatg gcgagtctag cgccgcgtta
     3361 aagatggcgc ctgcggtgct gatcaatcga gcacgttcag cggagacgga tgtcagcgcg
     3421 ggaacgaccc gttgcatggt tggtcgcaga ccgggcgctg cggggaacag cgtgccgagc
     3481 aattggccgg cgcagacgac gcgaacttcc gcggaggtgc ctggtccgcc agcggttcgt
     3541 ccaacttgat gcggttggta tcgggacacg cgattttcgg acaagatgag taaaaagaag
     3601 aagcgcggct ctgggtgagc cgcgcttctg gtcggtttcg tttccagcaa atcccgcgct
     3661 tttcggcggc tgttacttcg ccatttcctt cgcccgcgcc cgcacatcat ccagcccgcc
     3721 gacgtacagg aacgcttgtt ccggcaggtc gtccgcttcg ccgttgcaga tggcatcgaa
     3781 gctcttgagc gtttcctctc gcttcgtgta aatccccttc ttgccggtga attgctcggc
     3841 gacgaagaag ggttggctga agaagcgctc gatcttgcgg gcgcggctga cgatgagctt
     3901 gtcttcttcg ctgagttcat cgacgccgag gatggcgatg atgtcctgca gatcgcggta
     3961 gcgctgcagc atcgtctgaa ccttgcgcgc gatgcgggcg tgatcttcgc cgacgatgtc
     4021 ggccgacagc gcgcgcgatg ccgaagccag cgggtctacc gcggggtaga tgcctttgct
     4081 ggcgatgctg cgctcgaggt agatgaacgc gtccaggtgc gcgaaggcgt tggccggggc
     4141 ggggtcggtc gggtcgtcgg cggggacgta caccgcttgc acgctcgtga tggcgccgtt
     4201 cttcgtgctg gtgattcgct cctgcaattc gcccatttcc gtggccagcg tgggctgata
     4261 acccacggcg ctgggcatgc ggccgagcaa cgccgacact tccgaaccgg cctgcgtgaa
     4321 gcggaagatg ttgtcgatga acagcagcgt gtcggcgccg gtttcgtcgc ggaaatactc
     4381 ggccatcgtc agcgcgctca gcgccacgcg aagacgcgcc cccggcggtt cgttcatttg
     4441 gccgaagacc atcacggtct ggtcgatgac gtgcttaccg gtggtaccga tctcggcttc
     4501 ctgcatttcc agccaaaggt ccgtgccctc gcgggtgcgc tcaccgacgc cggcgaagac
     4561 ggaatagccg ccgtgctgct gcgcgatacg cgcgatcagt tcctgaatca ccacggtctt
     4621 accgagaccc gcgccgccga acaggcccga cttgccgccg cgaaggaatg gcgtcagcag
     4681 gtcgatcacc ttgatccccg tttcgaacac ctcggccttg ggcgacaggt ccacgaacgc
     4741 cggcggttcg cggtggatcc cgcggcggct ttcggtcacc accgggccgc ggttgtcgat
     4801 cggctcgccc agcaggttga acacgcgacc cagcgtttcc ttacccaccg gcaccgtgac
     4861 cggcccgccg gtgtcaaccg cgtccatgcc gcggcgaagt ccgtcggtcg aacccagcgc
     4921 gaccgcgcga accttcccgc cgcccaggtg ctgctgcact tcaccggtca ggcggatgtc
     4981 ggcctgcccg tcgttcttcg cgttgatcgt caccgcgttg taaatcttgg gcagctggtc
     5041 ttcggggtat tcaatgtcaa aggtggatcc gatgacctgc gtcacttttc cagtagcggg
     5101 catgagttcc tcgtcaagct cgtcgattgc cgcccgcgct gctcgaggcg cgcacgatca
     5161 atccgcaatc acacgcggcg cggaagaaat caaccaggcg caccgactgc gcccgatcac
     5221 ccgccacggt cacttcaggg gccggagatt gtattgaaac gtcacaaggt ggcaagggag
     5281 gcacaattcg cgccgttcgt acaaaagtcc ctgttctcac ggcaaagcgg ttgtcgtttc
     5341 agttaacaaa atgtaaacta ctgccgtgcg cgtgatcaag cggctgccct tgctggagtt
     5401 tggacatcaa cggccggccg cggtcaaacc actggatgca tggtatcgcg ccacgcttgc
     5461 tgccgactgg cgcaactttg cccaagtgaa ggcgacgttt ggctcgaccg atcaggcgaa
     5521 ggtggccagt ggacaaacgg tttgcatctt cggcatcggc gggaacaagt tccgcctgat
     5581 tgccttcgtg agttacgaaa agtcaaaggt atacgtcctg cgaatactca cccacaaaga
     5641 gtacgacagc aatcgctgga ggagcgagct atgaagtcgc tgacgaagcc taaccggcac
     5701 gatgccgccg gcgatacata cttggatttg gtccgccatc tgccgctgcg ccccattcgt
     5761 tctggcaagg attatgcgga ggctgccgct ctactcgacc gtttagcgct cctcgacgac
     5821 aagttggacg caggccagcg cgactatctg gaaacgctcg agatgctgat caacgcgtat
     5881 gacgatgctc atgcccgagt ggttccggac ggccgcacac cgctggagcg gttaaagtac
     5941 ctgatgcagc aaaacgcaat gacctcctcg gcacttggag atctactggg aaaccggccg
     6001 ctggcctcga tgatccttcg cggcaagcgc ggcctgagca aagcgcatat ccgcaagctc
     6061 gccgatcggt tcacggtaga cgccggctac ttcctgtaac acgtttatca atgcgagggc
     6121 ttccagcacg cgaccaccgc ggccgccgtt acggcggcgg tttcgatgcg caggatcgtc
     6181 gctgttagcg ccaccgcggt tgcgccggca gtggtgaact gatcgagctc gccatccgtc
     6241 cacccgcctt cggggccgat gaagatcgtt atcgccgccg gcagcgatgg cgactgcagc
     6301 agcttcgcga tcggcgtcgc cgcgccttgc gtggacatga cccatgtggg cgccgcgggg
     6361 gttgccgact tcagcacctc cgccag
//
LOCUS       DATIMH010000156         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4659570, whole genome shotgun sequence.
ACCESSION   DATIMH010000156 DATIMH010000000
VERSION     DATIMH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4659570"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VLI90_02625"
     CDS             <1..189
                     /locus_tag="VLI90_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13127.1"
                     /translation="QHVGRPRISRGRKAEKPEDAAQMQLFIDPAHELMRALGEAKMDE
                     LTPIQAFDLLRQWKEKYK"
     gene            695..>3008
                     /locus_tag="VLI90_02630"
     CDS             695..>3008
                     /locus_tag="VLI90_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HSV13128.1"
                     /translation="MKDMLRIPVALCVLISFLIGDHAVASAIRAVDRRPDGVGLATDG
                     GAVRLQVWSDRIVRVTCSPNADLPADESLSVISKPVATKWELRETPDAVIVETSALRA
                     KVDRKTGVVSFLDLQDQPVLQESPDARQFAPAATQAVSGVSVSDSFALAPDEHVYGLG
                     QHQGGVWNYRGQTVRLLQRNTDVALPVLLSSKGYGILWDNPAVTNIDVGVKGAEDRVR
                     WTSEVGKAVDYYFMYGPSSDEVVRDYRQLTGAAPMMGKWVWGFWQSKERYAKQHELID
                     TVSEYRKRGVPIDGIIQDWQYWKMDTWGSHAFDPTRYPDPAAMIKQLHDDLHARVLIS
                     VWPKFDLGTPNFDELEKAGAFYEPVIPYVYPKGKGKWYDAFNPVARRIYWKQISDQLF
                     SLGIDGWWLDASEAELSGKWGEFRNFKTAAGPGATVFNAYPLETTTAVYQGQRAQTDQ
                     KRVVILTRSAYAGQQRNAAITWSGDIQGNWDVLAKQIPAGLNFCASGIPYWNTDIGGF
                     FGKKPTDKKYQELFTRWFQFGAFCPMFRVHGTVAGKEFWQWDEPTQAIWKTYAGLRYR
                     LLPYIYSVSWQVTHDGGTMMRPLVMDFASDEKALDIGDQFLFGPSIMVNPVTKAGATT
                     RSVYLPGKLPWYDFWTGKRVESEQRMDAAAGIETMPLYVRAGAILPMGPTVQWTNEKP
                     ADPIELRVYRGADGTFTLYEDDGETYNYEKGQLATIPLTWSDAAGTLTIGARQGDFPG
                     MLKQRTFHVVFVTEGHGVGVDETAQADQTAN"
BASE COUNT          599 a          884 c          996 g          529 t
ORIGIN      
        1 caacacgtcg gccgcccccg catcagccgc ggccgaaagg cggagaagcc ggaagatgcg
       61 gcgcagatgc agctgttcat cgacccggcg cacgagctga tgcgggcgct gggcgaggcg
      121 aaaatggacg agctgacgcc gatccaggcg ttcgatctgc tgcggcagtg gaaagagaag
      181 tacaagtagc acgggctccg ggcctgtggc tctctcgccc atgtcttgcg atgctcgaca
      241 cgggcgagac gcccgtgcta caaagattgc tcggcgcggg agagtggacc atgtcgctca
      301 accgtcgtaa gcgcaataga ggaagtcgcc gcagcgcaag agcggcgtcg cgcatcgttg
      361 gcaagttgaa gtcgccgggt cgaacgcgag tacaacacaa gtcggagatc cctgccaaat
      421 gactccgtcg tgcggcaatg ttttcgaaga tgtcggcttc cgcgcggacg aagcgcaggt
      481 tctgctgctt ccttgtgagt tggcgctcac catcgagagg ttcaatggtc gatgcagccg
      541 cgcgcgatgt cgtagccgtc atgcgcaatc ctgagactgc cgcggaaaac caggacgcat
      601 cgcgcgcgtc tggcgctcat gtccagcgga gttggacacc ggcgcgtcac ccgtgctaca
      661 agagtgacaa ttcttccacc taccggaaca atcgatgaaa gacatgcttc gaatccccgt
      721 cgcgctgtgc gttctcatct cgttcttgat tggtgaccac gcggtggcgt cggcgattcg
      781 cgctgttgat cggcggccgg atggtgttgg cttggcgaca gacggtgggg cggtgcgact
      841 gcaggtttgg tctgatcgaa tcgtccgcgt gacgtgttca ccgaacgctg acttgccggc
      901 ggatgaaagc ttgagcgtca tcagcaaacc ggtggcgacg aagtgggaac tgcgcgaaac
      961 gccggatgcg gtgatcgtgg aaacttcggc gctgcgcgcg aaggtcgatc gcaagaccgg
     1021 cgtggtttca ttcctggacc tgcaagatca acccgttctt caagaatcgc ccgatgcgcg
     1081 tcaatttgcc cccgcggcaa cgcaggccgt cagcggcgtg agtgtctccg actcgttcgc
     1141 gctcgcgccg gatgaacacg tctacggcct cggccagcat cagggcggtg tttggaacta
     1201 ccgcgggcaa acggtccgcc tgcttcagcg gaacaccgac gtcgcgctgc cggtgctgct
     1261 gtcgagcaag ggctacggca tcctgtggga taacccggcg gtcaccaaca tcgacgtcgg
     1321 cgtcaaaggc gccgaagacc gcgtgcgctg gacgtccgaa gtcggcaagg cggtcgatta
     1381 ctacttcatg tatggcccat cttcggacga agtggtccga gattatcgcc agctcacggg
     1441 ggccgcgccg atgatgggca agtgggtgtg gggcttttgg caatccaagg aacgctacgc
     1501 caagcagcac gagctgatcg acaccgtcag cgaataccga aagcgcggcg tgccgatcga
     1561 cggcatcatc caggactggc aatactggaa gatggacacc tggggctcgc acgcgttcga
     1621 cccgacgcgc tatcccgacc cggcggcgat gatcaagcag ctgcacgatg acctgcacgc
     1681 gcgcgtgctg atttcggtct ggccgaagtt tgatctgggg acgccgaact tcgacgagct
     1741 ggagaaggcg ggcgcgtttt acgagccggt catcccgtac gtttatccca agggcaaggg
     1801 caagtggtac gacgcgttca acccggtggc tcgacggatt tactggaagc agatctcgga
     1861 tcaactgttc agcctcggca tcgacggctg gtggctggat gcgagcgagg cggaactgag
     1921 cggcaagtgg ggcgagttcc gcaatttcaa gaccgccgcc ggtcccgggg cgacggtgtt
     1981 caatgcctat ccgctcgaaa cgaccaccgc ggtgtaccag ggccaacgcg cccagacaga
     2041 tcagaagcgc gtcgtcattc tcacgcgcag tgcatacgcg gggcagcagc gtaacgccgc
     2101 catcacctgg tcgggcgata tccagggcaa ctgggacgtc ttggccaagc agatccccgc
     2161 ggggttgaac ttctgcgcca gcggaatccc ctactggaac accgacatcg gcgggttctt
     2221 cggcaagaag ccgaccgaca aaaaatacca ggagctattc acgcgctggt tccagttcgg
     2281 cgcgttctgc ccgatgttcc gcgtgcacgg caccgtcgcg gggaaggaat tctggcagtg
     2341 ggacgagccg acgcaggcga tctggaagac gtacgcgggg ctgcgctatc ggttgctgcc
     2401 gtacatctat tcggtcagct ggcaggtcac gcacgacggc ggcaccatga tgcgcccgct
     2461 ggtgatggat tttgcgtccg acgaaaaggc gctcgacatc ggcgaccaat tcctgttcgg
     2521 cccgtcgatc atggtgaatc ccgtcaccaa ggccggcgcg acgacgcgca gcgtgtatct
     2581 gccgggaaaa ttgccgtggt acgacttctg gaccggcaag cgcgtcgaga gcgagcagcg
     2641 catggacgcc gccgcgggga tcgagacgat gccgctgtac gttcgcgccg gcgcgatctt
     2701 accgatgggt cccacggtgc aatggacgaa tgagaaaccg gctgatccga tcgagctgcg
     2761 cgtctatcgc ggcgcggatg gcacattcac gctctatgaa gacgacggcg agacgtacaa
     2821 ctacgagaag ggccaactcg cgacgatccc gctgacgtgg agcgacgccg cgggaacact
     2881 gacgatcggc gctcggcagg gtgattttcc cgggatgctc aagcagcgga cgttccacgt
     2941 cgtctttgtg accgaaggtc acggcgttgg tgtggatgaa acggcgcagg cagatcagac
     3001 ggcgaatt
//
LOCUS       DATIMH010000157         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1193675, whole genome shotgun sequence.
ACCESSION   DATIMH010000157 DATIMH010000000
VERSION     DATIMH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193675"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VLI90_02635"
     CDS             complement(<1..625)
                     /locus_tag="VLI90_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13129.1"
                     /translation="MSFNPNLFPRNDCRAGVIVATLLATLLAMRSIVHADPTLLNVFA
                     NSGVGAETTPIYVTAGANGNVTVVDPFLGQLAGNVYAPDGNYRFTLLLPTGEMGLSNG
                     AAFGVTAADGSVYVADHSAGVAVFSPTGVQQRIIAGGVVQQPSGIAVTDSLVYVTNRT
                     SIAIYTTGGTSVGTFGSVGSGPGQFNIPNGIALDSTATNLYLADQTNN"
     gene            complement(828..>2219)
                     /locus_tag="VLI90_02640"
     CDS             complement(828..>2219)
                     /locus_tag="VLI90_02640"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HSV13130.1"
                     /translation="LKSDFSDPDAIRVGDDFYMVASDFHFVGMQVLHSKDLVNWQIIN
                     QIFHRLPIDAKYNQMTAYGQGTWAPSIRFHDGQFYVYVCTPLDGLFMWHTKDPAGKWS
                     GIVTVKAVPQWEDPCPFWDDDGQAYLIHSHKGAGPLIVHRMSADGTQLLDDGKEIYFH
                     KQAEGPKLYKRGGYYYVSFPESGVATGGQIVIRSKNIYGPYERKEVLPNGSPHQGGIV
                     DLDNGESWFIGFKSTGFLGRISYLEPVKWGDDGWPVFGDNGRPVQRWRKPNVGRQYPI
                     ERPPTSDDFDAKDLSPIWQWNHNPVDDHWSLSDRPGWLRLRGLPAEQLSLARNTLTEK
                     LSDENGTIDVRLDASHLADGQRAGFTFISGDKFGWVGVEQTNGERHIAWEGGQGPALT
                     SNEVWLRGTYHGDTARLSYCLDGKSFTDTKTKFALKFAFWKGARIGIFTYGGDGAADF
                     DYVHYRYADEGGQ"
BASE COUNT          379 a          670 c          726 g          444 t
ORIGIN      
        1 gattgttggt ctggtcggcg aggtacagat tggtcgcggt ggagtccagg gcgatgccgt
       61 tggggatgtt gaattgcccc ggtccactgc ccacgctgcc gaacgtaccg acgcttgtac
      121 cgccggtcgt gtagatggcg atgctggtgc gattggtgac gtacaccagg ctgtcggtga
      181 cggcgatgcc gctcggctgc tgcacgacgc cgccggcgat gatccgctgt tgcacgccgg
      241 tgggtgaaaa caccgccacg cccgcgctgt ggtcggcgac gtagaccgaa ccgtcggcgg
      301 cagtgacacc aaaggccgcc ccgttgctta accccatttc acccgttggc agcaacaacg
      361 tgaatcgata gttgccatcg ggcgcgtaga cgttgccggc cagctggccc aaaaatggat
      421 cgaccacggt gacgttgccg ttggccccgg cggtgacgta gatcggtgtc gtctcggcgc
      481 caacgccgct gttggcgaag acgttgagca gcgtgggatc agcgtggacg atgctgcgca
      541 tcgcgaggag cgtcgcgagg agcgtcgcga cgatcacacc ggcccgacaa tcgtttcggg
      601 ggaacagatt tgggttgaat gacacgatgg aactccctga tttgcgtgca gccgagcgtt
      661 ggatgccggc cgagtgcggg gagttacacg ttgagagaag ttacttggta atcacctcac
      721 gccgggttcg gagcgtactc gcgacgaccc ggattcttct gttctcgcgc cgcaggatcc
      781 gggccgtcgg ggtacctccg aacccggcgt ggaggttcat tgtgcgttca ctgcccgccc
      841 tcatctgcgt agcggtaatg gacgtaatca aaatcagcgg cgccgtcgcc gccgtaggtg
      901 aagatgccga tgcgggcgcc tttccagaat gcgaatttca gggcgaactt ggtcttggtg
      961 tcggtgaacg acttgccgtc caggcagtag ctcaatcgcg cggtgtcgcc gtgatacgtg
     1021 ccgcggagcc acacttcgtt tgacgtcagc gccgggcctt ggccgccttc ccaggcgatg
     1081 tgtcgctcgc cgttggtttg ctcgacgccg acccagccga acttgtcgcc gctgatgaag
     1141 gtgaagccgg cgcgctggcc gtcggccagg tgtgacgcgt cgaggcgcac gtcgatcgtg
     1201 ccgttctcgt ccgacagttt ttcagtgagc gtgtttcgcg ccagcgagag ttgctcggcc
     1261 ggcaggccgc gcaggcgcag ccagccgggg cggtcgctca gcgaccagtg atcgtccacc
     1321 ggattgtggt tccactgcca gatcggcgag agatccttcg catcgaaatc atcgctcgtt
     1381 ggcgggcgtt cgatcggata ctggcgcccg acgttcggct tgcgccagcg ctgcacgggc
     1441 cgaccgttgt cgccgaagac cggccagccg tcgtcgcccc atttgacggg ttcgagatag
     1501 ctgatccgcc cgagaaagcc ggtggatttg aagccgatga accacgactc gccgttgtcg
     1561 agatcgacaa tgccgccctg gtgcggcgaa ccgttgggca ggacttcctt gcgctcgtac
     1621 ggcccgtaaa tattcttcga acggatgacg atctgcccgc cggtggccac gccgctttcg
     1681 gggaacgaga cgtagtaata tccgccgcgc ttgtacagct tcggtccctc ggcttgcttg
     1741 tggaaataaa tttccttccc atcgtcgagc agctgcgtgc cgtccgcgct catccggtga
     1801 acgatcagcg gccccgcgcc cttgtggctg tggatcaggt acgcctgccc gtcgtcatcc
     1861 cagaacgggc atggatcctc ccactgcggc accgctttca ccgtgacgat gcccgaccat
     1921 ttccccgccg ggtccttcgt gtgccacatg aacagcccgt cgagcggcgt gcagacgtat
     1981 acgtaaaact gcccgtcgtg aaagcgaatc gacggcgccc acgtgccctg gccgtacgcg
     2041 gtcatctggt tgtacttcgc gtcgatcggc aggcgatgaa atatctggtt gatgatctgc
     2101 cagttgacca ggtccttcga gtgcagcacc tgcatgccga cgaagtggaa gtcggaggca
     2161 accatgtaga agtcatcgcc gacgcggatg gcgtcgggat cggaaaagtc ggatttgag
//
LOCUS       DATIMH010000158         1732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2347111, whole genome shotgun sequence.
ACCESSION   DATIMH010000158 DATIMH010000000
VERSION     DATIMH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2347111"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VLI90_02645"
     CDS             complement(<1..432)
                     /locus_tag="VLI90_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSV13131.1"
                     /translation="MTLPPPSVSVRERTTDAPSPAPTPPAAPKIDVQSLSFYYGAKRA
                     LEGITLRIQPNLVTAFIGPSGCGKSTFLRTLNRMNDILPGTRVEGKVLIEGQDVYAPT
                     VDVVELRRRVGMVFQKSNPFPKSIFENVAYGLRINRMTTSRN"
     gene            complement(441..1286)
                     /gene="pstA"
                     /locus_tag="VLI90_02650"
     CDS             complement(441..1286)
                     /gene="pstA"
                     /locus_tag="VLI90_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HSV13132.1"
                     /translation="MTRARWRRFISALFVGACGLAVLVALVPLGMILFFVVTQGVQAL
                     NLEFFTGMPKPVGEPGGGMLNAFVGTLILIAIGALIAIPVGVISGIYMSEYAGTRLAT
                     TARFAADTLNGVPSIVIGIFAYGIAVLPFRQFSALAGGLALGVMMIPIIARTTEELLL
                     LVPGSLREGALALGATRGRAVFSVIVPAALPGIITGVVLALARIAGETAPLLFTAFNN
                     QFFSTDIRQPIASMTVQIFTYAISPYEDWHRQAWAGALVLVSFVLVCSLLARIVTARV
                     QRLRG"
     gene            complement(1283..>1732)
                     /locus_tag="VLI90_02655"
     CDS             complement(1283..>1732)
                     /locus_tag="VLI90_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13133.1"
                     /translation="MVIPFTSTVAREVLKTVPVSQREAAYALGATRFEAIRAAMFFAR
                     TGILGAVMLGFGRALGETMAVTMVIGNNPQVSLSLFAPQYTMAAVIANEFTEAADRLY
                     LNALIEIGLVLFIITLLVNAGSRVLIWAMSRTPSPARRAASPAVVTT"
BASE COUNT          336 a          560 c          577 g          259 t
ORIGIN      
        1 gttgcggctg gtggtcatcc ggttgatccg cagtccatac gcgacgttct cgaagatcga
       61 tttggggaac gggttcgatt tctggaacac catgccgacc cgtcggcgca actcgacgac
      121 atcgacggtc ggcgcgtaga cgtcctgccc ctcgatcagg accttgccct cgacgcgggt
      181 gcccggcagg atgtcgttca tgcgattcag cgtgcgcagg aacgtgctct tgccgcatcc
      241 ggacggaccg atgaacgccg tcaccaggtt cggctggatg cgcaacgtga tcccttcgag
      301 ggctcgcttg gcaccgtagt agaaactcag cgactgcacg tcgatcttcg gtgcggccgg
      361 cggtgtcggt gccggtgacg gtgcgtcggt ggtgcgctcg cgaacgctga cggacggtgg
      421 tggaagagtc atcactgcgg ctacccccgc aaccgctgga cccgtgctgt cacgatgcgg
      481 gccagcagcg agcagacgag aacgaaggaa acgagcacca gagcgccagc ccacgcctgg
      541 cgatgccagt cctcgtacgg cgaaatcgca tacgtgaaga tctggacggt catcgacgcg
      601 atcggctggc ggatgtcggt cgagaagaac tggttgttga acgcggtgaa gagcagcggc
      661 gccgtttccc cggcaatacg cgccagcgcg agcaccacgc cggtgatgat accgggcaag
      721 gcggccggaa cgatgacgga gaagaccgcc cgtccccgcg tcgccccgag cgccagcgcc
      781 ccttcgcgca ggctgcccgg cacgagcagc agcagttctt cggtggtgcg cgcgatgatg
      841 gggatcatca tcacccccaa cgccaggcca ccagcgagtg cgctgaactg acgaaacggc
      901 agcacggcga tcccatacgc gaagatgcca atgacgatcg aagggacacc gttcagtgtg
      961 tccgcggcaa agcgcgccgt cgtggccaga cgggtcccgg cgtactccga catgtagatg
     1021 ccgctgatca cgccgaccgg gatggcgatc agcgccccga tggcgatcag aatcagggtg
     1081 ccgacgaacg cgttgagcat gccgccaccg ggctcaccca ccggcttcgg catgccggta
     1141 aagaactcga ggttcaacgc ctgcacgccc tgcgtgacga cgaagaacag gatcatcccc
     1201 agcggcacca gcgcgacgag tacggcaaga ccgcaggcgc cgacgaagag cgcggaaatg
     1261 aatcggcgcc agcgagcgcg ggtcatgtgg tcaccaccgc cggtgaggcg gcccggcggg
     1321 cgggcgaggg ggtccggctc atcgcccaga tcagcacgcg tgatcccgcg ttgaccagca
     1381 gcgtgatgat gaacaggacc aggccgattt caatcaacgc gttcaggtac agtcgatcgg
     1441 ccgcctcggt gaactcgttg gcgatcaccg ccgccatcgt gtactgcggc gcgaacagcg
     1501 acagcgacac ctgcggattg ttcccaatca ccatcgtcac ggccatcgtc tcgcccagtg
     1561 cgcggccgaa cccgagcatg acggcgccga ggatgccggt gcgtgcgaag aacatcgccg
     1621 cgcggatcgc ctcgaaccgt gtcgcgccga gcgcgtatgc tgcctcgcgc tgcgacaccg
     1681 gcacggtctt cagcacttcg cgggcgaccg tggacgtgaa cgggatgacc at
//
LOCUS       DATIMH010000159         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2347140, whole genome shotgun sequence.
ACCESSION   DATIMH010000159 DATIMH010000000
VERSION     DATIMH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2347140"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1414)
                     /locus_tag="VLI90_02660"
     CDS             complement(155..1414)
                     /locus_tag="VLI90_02660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV13134.1"
                     /translation="MPATRTRYILLYFACALSVITYIDRVCIGSSAPAIRAELGLTAV
                     QMGWVFSAFTLAYALFEIPSGWLGDVLGPRKTLMRIVLWWSAFTTASGIAWNFPSLLI
                     ARFLFGAGEAGAYPNMSRSFSRWFPATERGGAHGAVFMSSRLGGAIAPPLVILIISAA
                     GWRASFWIFGLFGIVWSVFWWHWFRDDPATHPSVSGDELAVIRAGTPPTALVRVHWRD
                     LLDVNLLCICTMYFCIGYGLYFYLTWLPTYFKEARGFSTKQAAWLSGAVLLTEAVAAM
                     AGGWLTDRLTARYGLKVGRSIGGIAMPLSGLFILSAAFTSHPIAAAVSIALAAGFADL
                     SLSPSWAMCHDVGGEAAGTVTGAMNTLGNLGGAISPLVVGYSVQMWGSWSRPLVITAF
                     VYGIGGVLTFLCNPHRRLVFREQNTWK"
     gene            1537..>2714
                     /locus_tag="VLI90_02665"
     CDS             1537..>2714
                     /locus_tag="VLI90_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV13135.1"
                     /translation="MSISTLRQDLRYTLRTLRRDAGFAAFAILIAGLGIGASATVFSV
                     LNALLLRPLPFATPERLVWVTNQDSSGLSGQTTQVGYMLDLRETTQTLSELAGYFAFY
                     GVGDNVLSGRGEPERLNGVPVSDNFFQVLGIQPQLGRTFTAEECQWHGPKAVLLSHSL
                     WARRFASDPAVVGTSVIINDEPQTIVGVLPESFDFASVFAPGSRFDLYFPFALSPETN
                     RWGNTMAMIGRLKPGVSASQAQSEIRTLGAQASREHPERNQFKGFVVPLSDHVNGKMR
                     PALWVLAGAVGVVMLIVCANLSNLLLARTASRQKEIAIRTALGASRRRLVAQMLTEGI
                     VLSSSGGALGLLLAIGGTRALAHLDAMNVPLLQRAHVDPATLLFTLVVAIVTGIVFGL
                     A"
BASE COUNT          523 a          874 c          855 g          462 t
ORIGIN      
        1 aaggtcgccg ccacgccgca caaaccgacc gcccaggcga atgccctcgt caaggccatt
       61 cgccatgcga cggatcgatt caggaacgtg acctctattt ctcccgtttg tcttttggtc
      121 ggtatgcagc atgacagtga acgttgctgg ggttctattt ccacgtgttc tgttcacgga
      181 ataccaatcg acgatgagga ttgcagagga acgtgaggac accgccgatg ccatagacga
      241 aggcggtgat caccagcggc ctggaccatg acccccacat ctgcaccgag tagccaacca
      301 ccagcggact gattgcgccg ccgagatttc cgagtgtgtt catggccccc gtgaccgtgc
      361 cggccgcttc gccgccgacg tcgtggcaca tcgcccagct cggactcaga gagagatcgg
      421 caaatccagc ggcaagggcg atcgacacgg ctgcggcgat cgggtgagag gtgaacgccg
      481 ctgacagaat gaacagcccg ctcagcggca tcgcaatgcc gccgatcgag cggccgactt
      541 tcaaaccata gcgcgcggtc agcctgtcgg tcagccatcc gcccgccatt gccgccaccg
      601 cttcggtgag gagcacggcg cccgacagcc acgcggcctg cttcgtgctg aaccctcgcg
      661 cttccttgaa atacgtcggc agccaggtca gatagaaata cagaccgtag ccgatgcaaa
      721 agtacatcgt gcagatacac agcaggttca catcgagcag atcgcgccag tgcacgcgca
      781 cgagtgcggt cgggggagtg cccgccctga tcaccgcgag ttcgtcgccg ctgaccgacg
      841 ggtgggtcgc gggatcgtcc cgaaaccaat gccaccagaa cacgctccag acgatgccaa
      901 atagaccgaa gatccaaaaa gacgcgcgcc accccgctgc cgagataatc aggatgacca
      961 gtggcggcgc aatcgctccg ccgagccggc tgctcatgaa caccgcgccg tgggccccgc
     1021 cccgttcggt cgcgggaaac catcgcgaga agctgcgcga catgtttgga taggcacccg
     1081 cctcgccggc gccgaaaaga aatcgcgcga tcaggagcga tgggaagttc cacgcgatgc
     1141 cgctcgcggt ggtgaacgcc gaccaccaca gcacaatacg catcaacgtc ttgcgcggac
     1201 cgagcacgtc acccagccag ccgctgggaa tttcgaatag cgcgtacgca agcgtaaacg
     1261 cgctgaacac ccaacccatt tgcacggcgg tcaatcccag ctcggcacga atggcaggcg
     1321 ccgacgagcc aatgcacacg cgatcgatat aagtgatcac cgatagcgcg caggcaaagt
     1381 acagaaggat gtagcgggta cgggtcgcag gcatgcggga tgcggcagtc gccggcggcg
     1441 gagtctatca tgacgggaag agctcgcctg gaggcggcgc ggcgaatatt gggaagcaga
     1501 cgccaacgct gccggcctcc gctccgtcaa aggagcatgt cgatctccac tctccgccag
     1561 gatctgcgct acacgctgcg aacccttcgg cgcgacgcgg gcttcgccgc gtttgcgatc
     1621 ctgattgccg gccttgggat cggggcgagc gccacagtgt tcagcgtgct caacgcgctg
     1681 ctgctccggc cgttgccgtt cgccaccccc gagcgcctgg tgtgggtgac caaccaggac
     1741 agcagcggct tgtccgggca gaccactcag gtcggctaca tgctcgatct gcgcgagacg
     1801 acgcaaacgc tctcggaact cgcagggtat ttcgccttct acggcgtcgg tgacaacgtg
     1861 ctgagcggcc gcggggagcc cgaacggttg aacggcgtcc ccgtctcgga caactttttt
     1921 caggtcctcg gcatccagcc gcagcttgga cgcaccttca ccgcggaaga gtgtcagtgg
     1981 cacggaccca aggcggtgct gctcagtcac agcctgtggg cgcgtcgctt tgcgtcggat
     2041 ccggcagtgg tgggaacctc ggtcatcatc aatgacgagc cgcagacgat tgtcggtgtg
     2101 ctccccgagt cattcgactt cgcgtcggtc ttcgcgcccg gcagccgttt cgatctttac
     2161 tttccgttcg ctctgagccc cgagacgaac cgatggggga atacgatggc gatgatcggc
     2221 cggttgaagc ccggcgtctc cgcgtcccag gcgcagtcgg agatcagaac gcttggcgcg
     2281 caggcctcgc gggagcatcc ggaacgaaac cagttcaaag gcttcgtggt gccgctcagc
     2341 gatcacgtca acggcaagat gcggccggct ctgtgggtgc tggccggcgc ggtcggggtc
     2401 gtgatgctca tcgtctgcgc gaacctctcg aacttgctgc tggcgcgcac cgcatcgcgg
     2461 cagaaagaga tcgcgatccg cacggcgctt ggcgccagcc gtcggcggct ggttgcccaa
     2521 atgctgaccg aagggattgt tctttcgtcg agcggcggcg cgctcgggtt gctcctggcg
     2581 atcggcggta cgcgcgcgct ggcgcatctt gacgcgatga acgttccgtt gctgcaacga
     2641 gcccacgtcg atccggcaac gctgctcttc acgctggtcg tcgcgatcgt gacgggcatc
     2701 gtgtttggac tcgc
//
LOCUS       DATIMH010000160         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5236576, whole genome shotgun sequence.
ACCESSION   DATIMH010000160 DATIMH010000000
VERSION     DATIMH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5236576"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VLI90_02670"
     CDS             <1..545
                     /locus_tag="VLI90_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13136.1"
                     /translation="DRILTLPIIAAFVVSLVHFLTLYRLRVPVKTAQMLGAMIAAMSV
                     QWTVSRAVAQGLITEHIAFARTSKGGLSSMSIEFQAFWEAVIGVLLLVGAAVLVVTNN
                     TKQIREIYIFAGVLVLQSLPFLAAVAIALLENSRTNSFAWWRDSAVRTAELIGIRPVR
                     LPTVAAPSQPVASEIRREAN"
     gene            773..848
                     /locus_tag="VLI90_02675"
     tRNA            773..848
                     /locus_tag="VLI90_02675"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:806..808,aa:Lys,seq:ttt)
     gene            1315..3135
                     /locus_tag="VLI90_02680"
     CDS             1315..3135
                     /locus_tag="VLI90_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HSV13137.1"
                     /translation="MSVAPLSEFEILEPAASNAAAVADERKVNLLVLCGLALVVGVMT
                     GCGAVALRALIGFFHNVFYNGTFSFLYDANVTEGPSRFGDFVFLSPILGGIVVVFLVE
                     RFAPEAKGHGVPEVMDAVFYKRGDIPGKVAAVKAVASALSIGSGAAVGREGPIIQIGA
                     ALGSAFAQLIKLSTWQKITLLSAGAGAGIAATFNTPLGGVLFALEILLPEVSNRTFLP
                     VVLATGAATTIGRILIGPDPAFVVPDVQFSLAQALGPQDAIAFVLLGILCGVASWAFI
                     RLLVIMEDTFPKLPGNAYTQNILGMAVIGLMMVMLTHLFGHPYVDGVGYGVIQSILNQ
                     KMSAAGLLALLFVLKMLATTISLGSGASGGIFSPSLYLGATLGASFAIVTGSVLPHAD
                     LTPSSAAIVGMAAMVGAGTGGVMTAIVMVFEMTRDYAIIVPVIVAVAVAAGIRRTLVG
                     ETIYTVKLRHRGHRIPKERHINLYLVKQAQDIMERRFIVAKAGTTLRQAMAEEDTDDL
                     RAIIVERDGRIVGLIPPRSGLWLESQTNPNLLVERFVESRVVVCRDQDLLSLVFARLK
                     RHRSGAAIIFHGVDRPRVRDVVGIITKRAIADAVIESYDD"
BASE COUNT          574 a         1005 c          992 g          703 t
ORIGIN      
        1 ctgaccggat cctgacgctg ccgatcatcg ccgcgttcgt ggtgtcgctg gtgcacttcc
       61 tgacgctgta ccgactccgc gtaccggtaa agacggcgca gatgctgggc gcgatgatcg
      121 ccgcgatgag cgtgcagtgg acggtgtcgc gcgcggtggc gcagggactg atcaccgagc
      181 acatcgcgtt cgcgcgcacg tccaagggcg gcctgtccag catgtcgatc gaattccagg
      241 cgttctggga agcggtgatc ggcgtactgt tgctggtggg cgccgcggtc cttgtcgtca
      301 ccaacaacac caagcagatt cgcgagatct acatcttcgc cggcgtgctg gtgctgcaga
      361 gcctgccgtt cctggccgcg gtcgcgatcg cgctgctgga gaactcccgc accaactcgt
      421 tcgcgtggtg gcgcgatagc gcggtgcgca cggcggaact gatcggcatc cgcccggtcc
      481 gcctgccgac ggtggcagcc ccctcgcagc cggtggcctc ggagattcgc cgggaagcca
      541 attaagacat agcgttttca agcgaagtgg ctaccggttc gcgcccggaa aacgcgtcgg
      601 ataaagtact gagcctctgc agaggctcag ccgtggacgg gtcgccgtat cagcttgaaa
      661 cggtgcggct aaccccaagc gagataacgg gaattcggag ctcaagacgg tggatttacc
      721 ccgctgccgc tattgaccca acggaccccg ccccctacac agcgcggcga gtgagcgcgt
      781 agctcaggtg gtagagcacg tgacttttaa tcatggggtc gagggttcga gtccctccgc
      841 gctcaccaaa catcaacgcg tttttgtttg actttatggt ggtcgcgacc ggccgcgtac
      901 aacttcgaag ctgggttgcg ttcacgcgct cggtcgcgat ttcctgacag agatggcgac
      961 agcgccgcta ggtgggttgc tctatatcgg acatggcctg ctggttgcac catgtaagct
     1021 ggctgaagtt ggcgcaatgg gcgatctgcc atagccgcct gtttggcaat tgtccgtcag
     1081 ccccaatcaa tccagcgcgg ttggtcgtct tgtccgatac cgaaatccga ctgtgctgct
     1141 tgtggacgcc ttgccagccc cctcacctca tcttcagcga taatagttcc gggcattgga
     1201 acccttcttg cctgccctcg ttgggactag cgttgctatg acgcccgctt cgatcgcggt
     1261 gcggggcgct ccctttcgat cgaacgtgaa ccgtgaacaa cctaccggat cgaaatgtct
     1321 gtagcgccgc tttccgaatt tgaaattctc gaaccggctg ccagcaacgc ggccgctgtg
     1381 gcagacgaaa gaaaggtcaa ccttctcgtt ctttgtgggc tcgcgctggt ggtgggtgtc
     1441 atgaccggat gcggcgcggt ggcactgcgc gcgctgatcg gatttttcca caacgtgttt
     1501 tacaacggta ccttcagttt cctttacgat gcaaacgtca cggaggggcc aagccggttc
     1561 ggggacttcg tctttctttc acccatcctc ggcggcatcg tcgtcgtttt cctcgtcgag
     1621 cgcttcgccc cggaagcaaa aggccatggc gtcccggaag tcatggatgc cgtgttttac
     1681 aaacgcggcg acattccggg caaggttgca gccgtgaagg cagtggcttc ggcgctttca
     1741 ataggaagtg gcgccgccgt cggccgcgag ggtccgatca ttcaaatcgg cgccgcgctg
     1801 ggctcggcat ttgcgcaact catcaaattg tcgacttggc aaaaaatcac cctgctctcg
     1861 gccggcgcag gcgcagggat cgcagcaacc ttcaatactc cgctgggcgg cgttcttttt
     1921 gccctggaaa tcctgttacc ggaggtctcc aaccgcacct tcctgcccgt cgtcctggcg
     1981 acgggcgccg ccaccaccat cggcagaata ctgatcgggc ccgatccggc tttcgtcgta
     2041 cccgatgtcc agttctcgct ggcccaagcg ctcggcccgc aggacgctat cgcctttgtt
     2101 ctgcttggta tcctgtgcgg cgtggcgtcc tgggcgttca tccgcctgct ggtgatcatg
     2161 gaagacacct ttccaaaatt gccgggaaac gcctacaccc agaatatcct gggcatggcg
     2221 gtcatcggac tgatgatggt tatgctcacg catctgttcg gccatcccta cgtggacggt
     2281 gtgggttacg gcgtcattca atcgatcctc aatcagaaaa tgtcagctgc cgggttgttg
     2341 gcgctgctgt tcgttctgaa gatgctcgcc accacgataa gcctcggatc gggagcttcc
     2401 ggcggaatct tctctccgtc gctctatctg ggcgccacgt tgggcgcgtc cttcgcgatc
     2461 gtcaccggtt cggttcttcc ccatgcagac ctgacgccct cctccgcggc gatcgtaggg
     2521 atggcagcga tggtgggtgc gggcaccggc ggcgtcatga cggcgatcgt catggtgttc
     2581 gagatgacgc gcgactacgc catcatcgtc ccggttatcg tcgcggtcgc ggtggcggcc
     2641 ggcattcgcc gcaccctggt cggcgaaacc atctacaccg taaagcttag gcatcgtggc
     2701 catcggattc cgaaggaacg acacatcaat ctctatctcg tgaagcaggc tcaggacatc
     2761 atggaacgcc gcttcatcgt cgccaaggcc ggaacgaccc tcaggcaggc aatggccgag
     2821 gaagacacag acgatttgcg cgcaataatc gtcgagcgcg acggccggat cgtcggactc
     2881 attccgccgc ggtccggtct ctggctggaa tcccagacca atccgaacct gctggtcgag
     2941 cgctttgtcg aaagccgcgt cgtcgtttgc cgggatcagg atttgctcag cctggtgttc
     3001 gcgagactga agcgacatcg ctccggcgca gcgattatct ttcacggcgt cgatcgccct
     3061 cgggtgcgag atgtcgtcgg aatcatcacg aagcgagcca tcgcggacgc ggtcatcgaa
     3121 agttacgacg actgattcct tgccagtgcg ctggaaagca ggcgccgacc ccggagaaca
     3181 tcatgcgtta cttgctatcc gccgtggctg ttgtcgtcgc gctttcgcag accgcttcgg
     3241 ctcagtcatc tcgggcagac cactccggcg gctt
//
LOCUS       DATIMH010000161         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4368377, whole genome shotgun sequence.
ACCESSION   DATIMH010000161 DATIMH010000000
VERSION     DATIMH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4368377"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VLI90_02685"
     CDS             complement(<1..250)
                     /locus_tag="VLI90_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13138.1"
                     /translation="MNTSTVHDKFEHVTLIRPRPGWIAINWRELWESRELLYFLVLRD
                     VKVRYKQTVLGVAWAVLQPVMSMLVFTVIFGRLAKMPSE"
     gene            complement(247..1758)
                     /locus_tag="VLI90_02690"
     CDS             complement(247..1758)
                     /locus_tag="VLI90_02690"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSV13139.1"
                     /translation="MLNNLKALVVVLAIGWAVFALCRPICLNFMTKEAFARRRNVWLG
                     LTIVAFVSPSFWIYAIVALIVLAWAAARDENPLALFAITLFVIPNVSFYIPTVLVNTL
                     FDLTQYRILSMAIVIPAMFRPKFGRDPPQSWFRREDAVLVAFLLLQVVLVMPYQSGTA
                     TMRKTLLLFMDIFVLFYSFSRLGTRERLTEVMACFWIGFGVMAPIAIFESMKGWLLYT
                     GIAGVWGDPNVFSWLFRGDSLRAQAAANHSINLGFHLSMALGFYLFLRTRGVDRFRDL
                     TVLATLIAGLVVTYARGPWVTAICLGVVFLATRPDAAKRLAGGLAVLGVLFALMYISP
                     LKESVLDRLPFIGTADQETIEYRRLLAEVSWTLIQQNPFFGDPFVASRMESLRNGQGI
                     IDIVNGYIYTALFNGLVGLALLVAFSLLALFRGFAAFRQCSDDKDAATLGAALLACMV
                     AALVYVATAGYNETLYVLFGLSVSYAFCCQSRVLAAPITRVVDQRRAMSSRTL"
     gene            complement(1809..>2270)
                     /locus_tag="VLI90_02695"
     CDS             complement(1809..>2270)
                     /locus_tag="VLI90_02695"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSV13140.1"
                     /translation="KLGIHADFRINEQLHLHRRLNVIIYLNAQWKPEWGGALELWDRE
                     MKAKVREVMPVFNRCVVFNTDADSFHGHPDPLCTPEGVFRRSIALYYYTASKEIYNEV
                     PSTSTMYHARPGDDATVRREARRLRFDEHIRQWVPPVLQRYVWALRRRLGR"
BASE COUNT          488 a          696 c          730 g          356 t
ORIGIN      
        1 cttccgacgg catctttgcc agtcgcccaa agattaccgt gaatacgagc atcgacatga
       61 ccggctgcag cacggcccaa gccacgccga ggacggtctg cttgtagcgc accttgacgt
      121 cgcgcaggac gaggaaatac agcagctcgc gcgattccca caactcgcgc cagttgatcg
      181 cgatccagcc gggccgcggc cggatcaacg tcacgtgctc gaacttgtcg tgcactgtcg
      241 aagtgttcat aaggttcgtg agctcatcgc cctgcgctgg tcgaccaccc gggtgatcgg
      301 cgctgcgagg acgcgacttt ggcagcaaaa cgcataggac acggacagcc cgaacaagac
      361 gtagagcgtc tcgttgtagc ccgcagttgc aacgtagacg agcgccgcaa ccatgcatgc
      421 caaaagcgcc gcgccaagcg tcgccgcatc tttgtcgtcg ctgcactggc gaaaggcggc
      481 gaagccgcga aacagcgcca gaagcgaaaa cgcgaccagc agcgccaaac ctaccaggcc
      541 gttgaagagc gcggtgtaga tgtagccgtt gacgatgtcg atgatgcctt gtccattgcg
      601 cagcgattcc atgcgcgacg ccacgaacgg atcgccgaag aacgggttct gctggatcag
      661 ggtccacgac acctccgcca gcaggcgtcg gtactcgatc gtttcctggt ctgcggtgcc
      721 gatgaacggc agacggtcga gcaccgattc cttgagtgga ctgatgtaca tcagggcgaa
      781 caggaccccg agcaccgcaa ggccaccggc gagacgcttc gcggcatccg gccgggtcgc
      841 caggaacacg acgcccaggc agatggcggt gacccacggc cctcgggcat aggtgacgac
      901 gagcccggcg atcagcgtgg caagcaccgt caggtccctg aagcggtcga cgcctcgcgt
      961 gcgcaggaag agatagaacc cgagcgccat cgacaagtga aagccgagat tgatcgaatg
     1021 gtttgccgcg gcctgggcgc gcagagagtc gccgcgaaac agccacgaaa agacgttcgg
     1081 gtcgccccag acgccggcga tgccggtgta gaggagccag cctttcatcg attcgaagat
     1141 tgcaatcggc gccatcacgc cgaacccgat ccagaagcac gccatgacct cggtcaatcg
     1201 ctcgcgggtg cccaggcggc tgaacgagta gaagagcacg aagatgtcca tgaacagcag
     1261 cagcgtcttg cgcatcgtcg ccgttccgct ctgatagggc atgacgagca cgacctggag
     1321 cagaagaaac gcgaccagga cagcgtcttc gcggcgaaac caggactgcg gcggatcacg
     1381 gccgaacttc gggcgaaaca tcgccggaat cacgatcgcc atcgacagga ttcgatactg
     1441 cgtcaggtcg aagagagtgt tgacgagaac cgtcgggatg tagaagctga cgttcgggat
     1501 cacgaacagc gtgatcgcga acagcgcgag cgggttctcg tcgcgagccg ctgcccaggc
     1561 cagcacgatc agcgcgacga tggcgtagat ccagaagctc gggctgacga aagcgacgat
     1621 cgtcaatccg agccagacgt tgcgtcggcg cgcgaacgcc tccttcgtca tgaagttcag
     1681 gcagatcggt cgacacaggg cgaataccgc ccagccgatg gccagaacga cgacgagcgc
     1741 cttcaggttg ttgagcatcg aggagatcgg ctagtcgcga tcgacgccgg ccggcgggtt
     1801 gccggggttc aacgaccaag ccggcgtcga agcgcccaga cgtagcgctg caggaccggc
     1861 ggcacccatt gacgaatatg ttcatcgaag cgcaggcggc gcgcctcccg gcgcaccgtc
     1921 gcgtcgtctc cgggccgggc gtggtacatc gtcgacgtgc tcggaacctc gttgtagatc
     1981 tctttcgacg cggtgtagta gtagagcgcg atcgagcgcc tgaagacgcc ttcgggcgtg
     2041 cacagcggat cggggtggcc gtggaacgag tcagcgtcgg tgttgaagac gacgcaacgg
     2101 ttgaagaccg gcatcacctc gcgcaccttg gccttcatct cgcggtccca cagctcgagc
     2161 gcgccgcccc actccggctt ccattgcgcg ttgaggtaga tgatcacgtt caggcggcga
     2221 tgcagatgca gctgctcgtt gatgcgaaag tcggcgtgga ttccaagctt
//
LOCUS       DATIMH010000162         3959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1482345, whole genome shotgun sequence.
ACCESSION   DATIMH010000162 DATIMH010000000
VERSION     DATIMH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482345"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..586)
                     /gene="rnpB"
                     /locus_tag="VLI90_02700"
     ncRNA           complement(94..586)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VLI90_02700"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            703..1545
                     /locus_tag="VLI90_02705"
     CDS             703..1545
                     /locus_tag="VLI90_02705"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HSV13141.1"
                     /translation="MASIDVQLLRNIPLFAKLTETELTELAGYLEPQPHQTNQSIFWI
                     GDKGTDFFIIQSGAVQLFYLDENGKEQPLATLKPGQFFGELSLLDGGPRTATARAASE
                     TTLLRLGRDNFERFLMTHAAAAFHILVTVGQRQRDMLDKLRGIKNANEIVAQEASRWH
                     RSADVVARAMSSPFFIMGQVAIVLTWVILARFEGANAFDPYPFNLLSLVMSVEALFLS
                     AFVLVSQGRQDERDRVQADLDYQVNLKAHMEVVQLNQKVDRIESLLRKQEARDESPML
                     AAGK"
     gene            complement(1610..2266)
                     /locus_tag="VLI90_02710"
     CDS             complement(1610..2266)
                     /locus_tag="VLI90_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSV13142.1"
                     /translation="MKIISNTGGVFATCSYLVADEIAKQAVIFDAPNDTVAPLLDEAQ
                     RNGWDVIGLWLTHGHIDHIADHAVVTSRFPNAKVLIHPLDERKLQTPQSIYPLPFETP
                     GRSADAHLSDGQQLRIGSIAVNVMHAPGHSPGHVVFHLPDEQVLIGGDVILMGAVGRT
                     DFPDCSFDALCASIRRIMQLPGETHLLPGHGHPSTLDHEMQTNECVQQAVAGNNGRTP
                     "
     gene            complement(2358..3383)
                     /locus_tag="VLI90_02715"
     CDS             complement(2358..3383)
                     /locus_tag="VLI90_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV13143.1"
                     /translation="MSQRIRWGILGTGNIATQFAHGVSHSSRGELAAVGSRKIESASA
                     FAKNNGIPTAHGSYDDLLRDHRVDAIYNSLPNSMHHEWTIKALRAGKHVLCEKPFARD
                     AGESQEMFDVARQTGKTVAEAFMYRSNPLTLAVKKAVDGGAIGTPHLIRTSFCYRTNK
                     IAGNIRFNRELAGGGLMDIGCYCINFARHFAGAEPSRVTASAHFHSSGVDDLLAGTMA
                     FPSGLIASFTCGMIAQADNTAYLCGSDGFIEIPVPWKPPAVEAIYHIGRGIPPKMDGP
                     IKPPAVTPRETVCVPIHGELYGMEADDFAAAVLDGAPPRVSEADTIGNMRVLDAIRRE
                     IKLEFST"
     gene            complement(3534..>3959)
                     /locus_tag="VLI90_02720"
     CDS             complement(3534..>3959)
                     /locus_tag="VLI90_02720"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV13144.1"
                     /translation="EQTHAAFAAAIIASYENSLDCPPLNGIRDVEDIIAGHKAAGEFD
                     PADWFVLMHHEAPVAVLLLSRTIHGDAMELVYLGVAPQARGHGLGDHLMRIAAHRMIE
                     RKIRRMTLAVDNLNTPALRLYHRHGLQRVASKLAMMRRL"
BASE COUNT          671 a         1247 c         1263 g          778 t
ORIGIN      
        1 gccgacgtgc gtgcgtacac gcaccacggg caggatgccc gtgctactaa tggaccggcg
       61 ggaccccttt caggaatccc gccggccagt gggtcgttgc ggtggaccgt aagccgaatt
      121 ctgtttcctc attgctgagg agacgatcat ttctctgtga cgcgcgtcgc cgcgcgcctc
      181 aggcgagcgc actcgccatg cgacctaccc gcggatcgta acgccccggg ccggggctgt
      241 ccgcatcttt ggtcgtgctg ccggtggggt ttgccgtgcc agcgatgtca ccaccgccgc
      301 ggtgcgctct taccgcacca tttcaccctt acctgaggaa atgcagattg atgaatgccg
      361 aatgcagaat gaagacggct ctgcattcat tccgcatcct gcattcatca atctgcattt
      421 cccccggcgg tatctttctg ttgcactttc cgtcgactcg ccagcatgct ggcttcgtcg
      481 ccttggcgtt accaagcacc gttgccctgt gcagttcgga ctttcctcac ccgtggcgtc
      541 gaacgtttcc gctcaaccca cgggcgcgat cgtcgcatcc gccgcaacgc gttcgattat
      601 agccgcaact cgccttgatg cgttcgttta gccgaatatc cttttgagcc gagtcgccgc
      661 caaacgcgcg ggaggcggct ctattgcggg agattcacgg gcatggcctc catcgacgtt
      721 cagctgctgc ggaacattcc gctgttcgcc aagcttaccg aaaccgagtt gaccgagctg
      781 gccggttacc tggagccgca accgcaccag acgaatcagt ccatcttctg gatcggcgac
      841 aaggggacgg acttcttcat catccagtcc ggcgcggtgc agctgtttta cctggacgag
      901 aacggcaagg aacagcccct ggccacgctg aagccggggc agtttttcgg cgagctgtcg
      961 ctgctggatg gcggaccgcg cacggcgacg gcgcgggccg cgtcggagac gacgttgctt
     1021 cgattgggcc gggacaactt cgagcggttt ctgatgaccc acgccgcggc ggcgtttcat
     1081 atactcgtga ccgtcgggca gcgccagcgc gacatgctcg acaagctgcg cgggatcaag
     1141 aacgccaacg agatcgtcgc gcaggaagcc tcgcgctggc accggtcggc cgacgtcgtc
     1201 gcccgcgcga tgtccagccc gttctttatc atgggccagg tggcgatcgt cctgacatgg
     1261 gtgatcctcg cccgcttcga aggcgccaac gcattcgatc cgtatccatt caatctgctg
     1321 tcgctggtga tgagcgtcga ggcgctgttc ctgtcggcgt tcgtgctggt cagccagggc
     1381 cggcaggacg agcgcgaccg cgttcaggcg gatttggatt accaggtgaa cctgaaggcc
     1441 cacatggagg tggtgcagct gaaccagaag gtggatcgga tcgagtcgct attgcgcaag
     1501 caggaggcga gggacgaatc gccgatgctt gccgcgggaa aataatagcc gggggcggga
     1561 gccccggtct gttcgaagga gaaaagaccg ggccttccgg ccccggctat tacggagtgc
     1621 gcccgttgtt tccagccacc gcctgctgca cgcactcgtt cgtctgcatc tcgtgatcca
     1681 gcgtgctcgg gtgcccgtgc cctggcagca ggtgcgtctc gcccggcagc tgcatgatcc
     1741 ggcggatcga cgcgcataac gcatcgaaac tgcaatccgg aaaatccgtt cgacccacag
     1801 cgcccatcaa gattacatcg ccgccgatca ggacctgttc gtccggcaga tgaaacacga
     1861 cgtgccccgg tgaatgcccg ggggcatgca tcacgttcac cgcgatcgat ccgatgcgca
     1921 gctgctgccc gtcgctcaga tgcgcatccg ccgatcgccc cggcgtctcg aacggcagcg
     1981 gatagatgct ctgcggcgtc tgcagcttcc gttcgtccag cggatgaatc agcaccttcg
     2041 cattcggaaa ccgcgacgtg accaccgcgt ggtcagcgat gtggtcgatg tgcccgtgcg
     2101 tgagccaaag gccgatgacg tcccagccgt tcctctgcgc ctcgtcgagc agcggggcaa
     2161 cggtgtcgtt gggcgcgtcg aagatcaccg cctgcttcgc gatttcgtcg gcgacgagat
     2221 acgagcaagt ggcgaacacg ccgccggtgt tgctgatgat cttcatggga tgccaagtcg
     2281 ctggacggat aatagccggg ggcggaagcc cggtcctttt tgcgttcgaa cgggatcggg
     2341 gcttccgccc tcggctatta agtcgagaac tccaacttga tctcgcgccg gattgcatcc
     2401 agcacgcgca tgttgccgat cgtatccgcc tcgctcacgc gcggcggcgc gccatccagc
     2461 accgcggcgg cgaaatcgtc ggcttccatg ccgtacaatt cgccgtggat cggcacgcaa
     2521 accgtttcgc gcggcgtcac cgccggtggt tttatcgggc cgtccatctt cggtgggatc
     2581 ccgcggccga tgtggtagat cgcctccacc gcgggcggtt tccacggcac ggggatttca
     2641 ataaagccat cgctgccgca caggtacgcc gtgttgtcgg cttgcgcgat catcccgcat
     2701 gtgaagctgg cgatcagacc gctggggaac gccatcgtgc cggccagcag gtcatccacg
     2761 ccgctgctgt gaaagtgcgc gctggccgtg acgcgcgacg gttcggcgcc ggcgaaatgg
     2821 cgcgcgaagt tgatgcagta gcagccgatg tccatcaacc caccgccggc cagctcgcgg
     2881 ttgaagcgga tgttcccggc gatcttgttc gtgcgatagc agaagctggt gcggatgaga
     2941 tgtggcgtac cgatcgcgcc gccatccacc gccttcttca ccgcgagcgt cagcgggttg
     3001 ctgcggtaca tgaacgcttc ggcgacggtt tttcccgtct gtcgcgccac gtcgaacatc
     3061 tcctgtgatt cgcccgcatc gcgcgcgaac ggcttttcgc acagcacgtg ctttccggcg
     3121 cgcagcgctt tgatcgtcca ctcgtgatgc atcgagttcg gcagcgagtt gtagatcgcg
     3181 tcgacgcgat ggtcgcgcag cagatcgtcg taactgccat gggcggtggg gatgccgttg
     3241 ttcttcgcga acgccgacgc cgattcgatc tttcgcgagc caacggcggc tagttcgccg
     3301 cgggacgagt gcgatacgcc gtgcgcgaat tgcgtggcga tgttaccggt gccgaggatc
     3361 ccccagcgga tgcgttgaga catggcagaa gcatagccgg ggtgattggg agtcgaaatt
     3421 caaaattcga gttgggggaa gatgacgtcg tttagccggc gcgggctcgc ggtgctcttc
     3481 tacgtccgcg ccgcgaggcc gcggcggcta aaggatgagg ggcgcgcccg cgctcacagc
     3541 cgccgcatca tcgcgagctt gctcgcgacg cgttgcaggc cgtggcggtg gtacagccgc
     3601 agcgcaggcg tgttcaggtt gtctaccgcc agcgtcatcc ggcgaatctt tcgctcgatc
     3661 atgcgatggg cggcgatgcg catcagatga tcacccagcc cgtgcccgcg cgcttggggg
     3721 gcgacgccga gatacaccaa ctccatcgca tcgccatgaa tcgttcggct cagcagcaac
     3781 accgcgaccg gcgcctcgtg atgcatcagc acgaaccagt cggccggatc gaattcaccc
     3841 gcggctttgt ggccggcgat gatgtcttca acatctcgaa tgccgttgag gggcgggcag
     3901 tccaggctgt tctcgtagct tgcgatgatg gcggccgcga aggcggcgtg ggtttgctc
//
LOCUS       DATIMH010000163         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3792309, whole genome shotgun sequence.
ACCESSION   DATIMH010000163 DATIMH010000000
VERSION     DATIMH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3792309"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VLI90_02725"
     CDS             <1..299
                     /locus_tag="VLI90_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13145.1"
                     /translation="YCVPAVRAVKIYQRNHGQMPASVDALGPPFSSHHTDGPGIHMID
                     ALRPYTYVAFGSYQHTISYDFTPGEEGWTVYGPYANGPVPVPPVVLEPVPATRP"
     gene            609..1040
                     /locus_tag="VLI90_02730"
     CDS             609..1040
                     /locus_tag="VLI90_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13146.1"
                     /translation="MNASTRRFKYYTDPLCIACLLVYPINRWYLKPHHIGGWFTHGYL
                     NDVLCLPLFLPMILYVQRLIGLRKHDGYPRWWEILQHWAIYTVVFEVILPTMPNVFTH
                     TQDPWNAVAYLAGAVIAEVFWNKAGQNARRQPRDARGANTD"
     gene            1110..1322
                     /locus_tag="VLI90_02735"
     CDS             1110..1322
                     /locus_tag="VLI90_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13147.1"
                     /translation="MTGDLDRPNIDQVDSPELDLVVEAYKSGVDKTLILENLRLTPQQ
                     RVEKLIALHQFDDELRNAIAGRSRGG"
     gene            1319..1828
                     /locus_tag="VLI90_02740"
     CDS             1319..1828
                     /locus_tag="VLI90_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13148.1"
                     /translation="MTDFKALFTCFADSGIDVIVIGGVAARAHGSARFTEDLDVVYLR
                     TPENIRRMVRALDSHSPYLRGAPPGLPFRWDERTITRGLNFTLITDLGAIDLIGEVTG
                     GGHYEDLIEHTVSAELFGHSYRCLDLDWLIRVKRAAGRPKDLEALAELEVIRDAKMPH
                     PPTAGEFAK"
     gene            1938..3431
                     /gene="pyk"
                     /locus_tag="VLI90_02745"
     CDS             1938..3431
                     /gene="pyk"
                     /locus_tag="VLI90_02745"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSV13149.1"
                     /translation="MIRTKIVATMGPACGDVDTLLRLFEAGVDVCRLNFSHGALDGHL
                     LTLRNIREAASRHTAPIAILGDLGGPKIRLGQIKDTNAAGGMPIHVGDTLIIQREPML
                     GENGRVSSTYPDMIDEVCVGQRVLIEDGLLRFVCTDKTANELICSCTAGGILKNSKGI
                     NLPDTTVNVPSITERDWECIDWAIEQRLDYLALSFVRKADDIRELRDYLSSKVSDIHL
                     IAKIEKAEAVDHIDEIIDVADGLMVARGDLGVEMDVAEVPIIQKDLIRRCQSAGKPVI
                     VATQMLQSMVEQSSPTRAEVSDVANAIFDGTDAVMLSGETSVGKFPVVTVHTMSHIAD
                     VTETYLAKVDASEVAPPLVKTTLPAGALARGTLRVVHDLKAKLVVVWTQTGTTARVFS
                     KHHFPVPIIALSPDHRTLRRMALHYGVIPQEMQATPDLTNLIARIDEFIISRQYAEPK
                     DRVIIVAGWSPGTPGTMNGLVIHTVGESWTPVPTAQMLRQMVKTEKE"
     gene            3580..>4078
                     /locus_tag="VLI90_02750"
     CDS             3580..>4078
                     /locus_tag="VLI90_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13150.1"
                     /translation="MLAQLRCALVSSAALALLAGCIVAPANPAIKERVTNVDPKLATP
                     DYWLNQPAVTRISDGDFYKLWNACRDEVFSRFFQIDREEYRDGLLTTQPMVTKQFFEV
                     WRRDAVTIKDIADASVGTIRRTVRFQLTRKDDGTYVAEPKVLVERFSSAERRLTAIHE
                     YHAAFS"
BASE COUNT          830 a         1230 c         1209 g          809 t
ORIGIN      
        1 agtactgcgt gcctgccgtg cgcgccgtca agatctacca gcgaaatcat ggacaaatgc
       61 ccgcaagcgt tgacgcgttg ggaccgccat tttcgagtca ccacaccgat ggaccgggga
      121 tacacatgat tgatgcgctt cgaccttaca cctatgtggc atttggttcg tatcagcaca
      181 caatcagcta cgacttcacc ccaggcgaag agggttggac cgtttacggc ccctacgcca
      241 acggacctgt tcccgtcccg ccggtggtct tggaaccggt ccccgcaaca aggccatgag
      301 taagatttca tgtactgcaa acgctgctta gccgcgcttc cccaatcctt ccgtgatatg
      361 ccggcagctc ctgaggcgcc atccgatccg aagccggcag tgctggagta cgcatcaccc
      421 cgcaagaagc cgaacagatg tcgccggtgc tttcgaaaat tcgattcaga tgaccccaag
      481 acctacctct cgaccctggc ccttacaccg cgccagatgg tactccgggt gatcttgacc
      541 acgctgattg ggattgccgc ggcattcgtc gttgcgtttc atcaattggt ggcacacagc
      601 gggcactgat gaacgcgagc acacgacggt tcaagtatta caccgatccg ttgtgcatag
      661 cctgtctgct cgtctatccg atcaaccggt ggtacctcaa gccacaccac atcggcggtt
      721 ggttcacgca tggctacctg aatgacgtgc tgtgcctgcc gctattcttg ccaatgatcc
      781 tgtacgtcca gcggctgatt ggccttcgca agcacgatgg gtatccacgc tggtgggaaa
      841 tcctgcagca ctgggcgatc tatacggtgg tcttcgaagt gatacttcca acgatgccga
      901 acgtgttcac acacacgcag gatccgtgga atgcggtggc atatcttgcg ggcgcggtga
      961 tcgcagaggt cttctggaac aaggcaggcc aaaacgcaag gcggcagcca cgtgacgcgc
     1021 ggggcgccaa taccgactga agaaaagcgg ttgaaggaag cggacatcga cctgtttgtg
     1081 ccctcagaaa acgcgtagaa tacggcgcca tgacaggtga tctggaccga ccaaacattg
     1141 accaagttga ctcgcccgag cttgatcttg tggttgaggc ctataaatcc ggagtcgaca
     1201 aaacgcttat tttggaaaat ctgcggctaa cgccgcagca gcgcgtggag aagctgatag
     1261 cgctgcatca atttgacgat gagctgcgga acgcaatcgc gggcaggagc cgaggcggat
     1321 gactgatttc aaggctctgt tcacctgttt tgctgattca ggtatagacg tcatcgtcat
     1381 cggtggcgtt gcggctcgcg ctcatgggag cgcgcgattc actgaagatc tcgacgtcgt
     1441 ctatttgcgc acgccggaaa acattcggcg aatggttcgt gcgctcgatt cacattcgcc
     1501 ttacttgcgc ggtgctccac caggacttcc gttcaggtgg gatgagcgta cgatcacacg
     1561 aggactcaac ttcacgctca ttaccgactt gggagccatt gatcttattg gcgaggtgac
     1621 cggtggtggg cattacgaag atctgattga acatactgtc agcgccgaac tgttcggtca
     1681 ctcataccgg tgtctcgatc tcgattggct cattcgcgtt aagcgtgcgg ctggacgacc
     1741 gaaggatctg gaagcactgg cagagttaga agtgattcgc gacgcgaaga tgccgcatcc
     1801 acccaccgcg ggcgaattcg ccaaatgagt gcaattcacc cgtaacggtc tgtgcgcctg
     1861 ctcttccctc gcgttcgccg ttccgtatga tgcagttcgc acatggaact cgcgcgcagt
     1921 tgccgatgaa ccacgcaatg atccgtacca agattgtcgc caccatgggc cccgcctgtg
     1981 gggatgttga cacgctgctg cgcctgttcg aagcgggcgt ggatgtctgc cgattgaatt
     2041 tttcgcacgg ggcgctggat gggcacttgc tgacgctgcg caacatccgc gaagcggcca
     2101 gccggcatac cgcgccgatt gcgattctcg gtgatctggg tggcccgaaa atccgcctcg
     2161 gccagatcaa ggacaccaac gccgccggcg ggatgccgat ccacgtcggc gatacgttga
     2221 tcattcagcg cgagccgatg ctcggcgaaa acggccgcgt ttcctcgaca tatccggaca
     2281 tgatcgacga ggtctgtgtt ggtcagcgcg tgttgatcga ggatgggttg cttcgcttcg
     2341 tctgcacgga caaaaccgca aacgagttga tctgcagctg caccgccggg ggaattctca
     2401 aaaattccaa gggcatcaac ctgcccgata cgacggtgaa cgtgccgtcg atcaccgagc
     2461 gcgattggga gtgcatcgac tgggccatcg agcagcggct ggattacctg gcgctgtcgt
     2521 tcgtgcgcaa ggccgacgac attcgcgagc tgcgcgatta cctgagcagc aaggtcagcg
     2581 acatccatct gatcgccaag atcgagaagg ccgaagccgt cgatcacatc gacgaaatca
     2641 tcgacgtcgc cgacggttta atggtcgccc gcggtgactt gggcgttgag atggacgtgg
     2701 ccgaggtgcc gatcatccag aaagatctga tccgccgctg ccagagcgct ggaaagccgg
     2761 tgatcgtcgc gacgcagatg cttcagagca tggtcgagca aagctccccg acgcgcgcgg
     2821 aagtgagcga cgtggccaac gccatcttcg acggcaccga cgccgtcatg ctcagcggtg
     2881 aaaccagcgt gggcaaattc cccgtcgtca ccgtgcacac gatgtcgcac atcgcggatg
     2941 tgaccgagac ctatctggcg aaggtggatg cgtcggaagt cgcgccgcca ctggtaaaga
     3001 caacgctgcc cgccggggcg ctggcgcgcg gcacgctgcg cgtcgtgcac gatctgaaag
     3061 caaaacttgt cgtcgtctgg acgcagacgg gaaccaccgc tcgcgtgttt tccaagcacc
     3121 atttcccggt gccgatcatc gcgctctcgc ccgaccaccg cacgttgcgg cggatggcgc
     3181 tgcactatgg cgtcatcccg caggaaatgc aggcgacgcc ggatttgacg aacctgatcg
     3241 cgcggatcga cgagttcatc atctcgcggc aatacgccga gccgaaggac cgcgtgatca
     3301 tcgtcgccgg ctggtcgccg gggacgccgg gcacgatgaa cgggttggtc atccacacgg
     3361 tgggtgaatc gtggacgccg gtgccgaccg cgcagatgct gcggcagatg gtgaagacgg
     3421 agaaggaatg agcgcaccca ccgctaccca acacgctgac gaacgtgcgc gagcagagag
     3481 cccagcggct atgccgcggg gcgtcttgtg atgtgcgttg tttgtgcgag taacgtcttt
     3541 tcgacttccg cgcgtcccgc ggcggagccg ctgggctctg tgctggcgca gcttcggtgc
     3601 gcccttgttt catccgccgc gctcgcgttg ctcgccggct gcatcgtcgc ccccgccaac
     3661 cccgccatca aagagcgcgt caccaacgtt gatcccaagc tcgccacgcc ggactattgg
     3721 ctcaaccagc ccgccgtcac gcgcatcagc gatggcgact tctacaagct ctggaacgcc
     3781 tgccgcgatg aggtgttttc tcgcttcttt cagatcgatc gcgaggaata ccgcgatggc
     3841 ttgctgacga cccagccgat ggtcaccaag cagttcttcg aggtctggcg gcgtgatgcg
     3901 gtgaccatca aagacatcgc cgacgcctct gtcggcacca ttcgccggac cgttcgcttt
     3961 caactcacgc gcaaggatga cggcacgtac gtggccgaac ccaaggtgct cgtcgaacga
     4021 ttctcctccg ccgagcgccg cctcaccgcg atccacgaat atcacgcggc cttcagcg
//
LOCUS       DATIMH010000164         4193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1194253, whole genome shotgun sequence.
ACCESSION   DATIMH010000164 DATIMH010000000
VERSION     DATIMH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194253"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VLI90_02755"
     CDS             complement(<1..590)
                     /locus_tag="VLI90_02755"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HSV13151.1"
                     /translation="MATQKRTLGRRTAGLLMHVSSLPGPYGVGDLGPSAYRWIHALSR
                     AKQSWWQLLPLGYGESPYQCLSAFAGSPNLISPAALVQDGLLTRAQTQPPPLPADHVN
                     FPRVRQLKSRLLQRAWEHFNADSRVRRKFAGAFEKFRNQQAHWLDDFALFMALKESAA
                     GRAWFDWSRDLVMRKPDALRSAQHEFADQIDRHRFT"
     gene            815..3034
                     /gene="glgX"
                     /locus_tag="VLI90_02760"
     CDS             815..3034
                     /gene="glgX"
                     /locus_tag="VLI90_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HSV13152.1"
                     /translation="MRVWPGRPYPLGATWDGVGVNFALFSEHATKVELCLFDDIDSTK
                     ESQRIPLPEVTDMVWHGYFPDLEPAQLYGYRVHGPYEPEKGHRFNPNKVVLDPYAKAI
                     GRDLTWDDSLFGYGIGNEHKDLSFDERDSAAFAPLAAVIDTAFTWGDDRPPRTPWHKT
                     LIYEMHIKGFTYQNPEIPEHQRGTYAGVASEASIAHLQSLQVTAVELLPVHFHLNDRH
                     LVEKGLTNYWGYNTLGFFAPHVAYASKQSPRKSVQEFKMMVRALHAAGIEVILDVVYN
                     HTSEGSEMGPTLSWRGIDNASYYRLADDLRYHMDFTGCGNTLKMVNPRVLQMIMDSLR
                     YWVLEMHVDGFRFDLASTLARELHEVDRLGAFFDIIHQDPVLSQVKLIAEPWDVGPGG
                     YQVGNFPVLWTEWNGKYRDTVRRFWKGDGGLASEFATRLAGSSDLYQRDGRKPYASIN
                     FITCHDGFTLQDLVSYDQKHNEANGEDNRDGADDNNSWNCGAEGPTDDQNIINVRERQ
                     KRNLIATLLFSEGVPMIFAGDEMSHTKKGNNNTYCQDNELSWVNWDLDERQRKFLDFV
                     RQCCQIWGEQPVFQRRNFFSGRAIRGSEIKDISFFEPSGQEMTDAAWNTGFVKCLGVR
                     LAGDIINEVDERGEPIVGDTLLMIMNAHWEKLDFTLPAARVEHVWETMLDTSDPDLPL
                     RVCRAGENYPLLGRSLALLRTCKPEETGAVSPAQIETLRRSAERANQPSPKEPPLLR"
     gene            3064..>4193
                     /locus_tag="VLI90_02765"
     CDS             3064..>4193
                     /locus_tag="VLI90_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSV13153.1"
                     /translation="MMVRESVQEQVAPPVASNRRMPIGAEALPGGGVSFRVWAPGHRD
                     VSVVFEDRSIAELPLAPESDGYFAGVSAEAGAGSRYRYRLDGEENFYPDPASRSQPDG
                     PHGPSQVIDPTAFQWTDDDWPGVGAAGQVLYEMHVGTFTTEGTWAAAERELPALKELG
                     VTCLEVMPVADFAGTFGWGYDGVNLFAPTRLYGSPDDFRRFVDRAHRVGIGVILDLVY
                     NHLGPDGNYLTKFTPDYFNAEHKTDWGDALNFDGRNSGGVRELYLSNARYWIEEFHLD
                     GFRFDATQAIVDSSSRHILAEITETSRRAAGKRSVYLVNENEPQHTRLVGPVDAGGYG
                     MDALWNDDFHHSAMVALSGHNEAYYSDYLGKPQELLAAAKWG"
BASE COUNT          816 a         1194 c         1408 g          775 t
ORIGIN      
        1 tgcgtaaagc ggtggcggtc gatctggtcg gcgaattcat gctgagcgga tcgcagggcg
       61 tcgggcttgc gcatgaccag gtcgcgcgac caatcgaacc acgcgcgccc ggcggcggat
      121 tctttcagcg ccatgaagag cgcgaagtcg tcgagccaat gggcttgttg gttgcgaaac
      181 ttttcgaacg cgccggcgaa cttgcgtcgg acgcgactgt cggcgttgaa atgttcccac
      241 gcgcgttgca gcaggcggga cttcaactgg cgaacgcgtg ggaagtttac gtgatcggcc
      301 ggcaacggtg ggggctgtgt ctgggcgcgg gttagcaatc catcttgcac gagcgccgcg
      361 gggctgatca ggttgggtga accggcgaag gctgagagac attggtaagg cgattcgcca
      421 tagcccaggg gcagcagctg ccaccaggac tgcttcgctc gtgacaatgc atggatccat
      481 cgataagcgg atggaccgag atcaccgacg ccgtaaggcc cgggcaggct gctgacgtgc
      541 atcagcagac ctgcggttcg gcgtccgagt gttcgctttt gcgtcgccat caccttcgcc
      601 ttgtagcgcc atttttgccg cggccagaac gccggggttc atgtcggtgc ggacacactg
      661 acgcaccggc catatccgcg tggttcagga acataggaag aatatgcggt gttgacggcg
      721 ctgaatttgg aatcggcgga tggcaccccg tttgttctat gttacacgat tgttgagccc
      781 gcaaaaacaa ggtagtcgga aaggtaaacg aaacatgaga gtgtggcctg gacggccgta
      841 tccattgggc gcgacgtggg acggcgtggg tgttaacttc gcgctctttt cggagcatgc
      901 gaccaaggtc gaactgtgtc tgttcgacga cattgattcc acgaaagaat cccaacgcat
      961 tccgttgccg gaagtgacgg acatggtctg gcacgggtac ttcccggacc tggagccggc
     1021 gcagctgtat ggctatcgcg tgcacgggcc gtatgaaccg gagaaggggc atcgcttcaa
     1081 tccgaacaag gtggtgctcg atccgtatgc gaaggcgatc ggtcgcgatc tgacatggga
     1141 tgattcgctg ttcggttacg gcatcggcaa tgagcataaa gatctgtcgt tcgatgaacg
     1201 cgacagcgcg gcgttcgcgc cgctggcggc ggtgatcgac acggcgttca cttggggtga
     1261 cgaccgtccg ccgcgcacgc cgtggcataa gacgctcatc tacgagatgc acatcaaggg
     1321 tttcacgtac cagaacccgg agatccccga gcaccagcgt ggcacctacg cgggcgtggc
     1381 atctgaagcg tcgattgcgc atctgcaatc gctgcaagtg accgcggtgg aactgctgcc
     1441 ggtgcatttt catttgaacg atcggcacct ggttgaaaag gggttgacga attattgggg
     1501 atacaacacg ctgggctttt tcgcgccgca tgtggcatat gcgtcgaagc agtcgccgcg
     1561 caagagcgtg caggaattca agatgatggt gcgcgcgctg cacgcggcgg gcatcgaggt
     1621 gattctcgac gtcgtttaca accacacttc cgaaggcagc gagatggggc cgacgctgtc
     1681 ttggcgcggc atcgacaacg cctcgtatta tcgattggct gacgatctgc gttaccacat
     1741 ggacttcacc ggttgcggca acacgctgaa gatggtgaac ccgcgcgtgc tgcagatgat
     1801 catggacagc ctgcgctact gggtgctgga gatgcacgtg gacggcttcc gcttcgacct
     1861 tgcgagcacg ctcgcgcgcg aattgcacga agtggatcgg ctgggcgcgt tcttcgacat
     1921 cattcaccag gatccggtgc tgtcgcaggt gaagctgatc gccgagccgt gggacgtcgg
     1981 gccgggtggg tatcaggtcg ggaacttccc cgtgctgtgg acggagtgga acgggaaata
     2041 tcgcgatacg gtgcgacggt tctggaaagg cgatggtggg cttgcatcgg aattcgcgac
     2101 gcggctggcg ggcagcagcg atctttatca gcgcgacggt cgaaagcctt acgccagcat
     2161 caacttcatc acctgccacg acggcttcac gctgcaggac ctggtcagct acgaccagaa
     2221 gcacaacgag gccaacggcg aagacaaccg cgacggcgcg gatgacaaca acagctggaa
     2281 ctgcggcgcc gagggtccga ccgacgatca gaacatcatc aacgtgcgcg agcggcagaa
     2341 gcggaacctg atcgcgacgc tgctgttctc cgaaggcgtg ccgatgatct tcgctggcga
     2401 cgagatgagc cacacgaaga aggggaacaa caacacctat tgccaggaca acgaactgag
     2461 ttgggtcaat tgggacttgg acgagcggca gcgcaagttc ctggacttcg tccgccagtg
     2521 ttgccagatc tggggcgagc agccggtgtt ccagcgccgg aactttttca gtggccgagc
     2581 catccgcggg tcggagatca aggacatctc gttcttcgaa ccgtcgggtc aggaaatgac
     2641 cgacgcggcg tggaacaccg gatttgttaa atgtctcggc gtccgattgg cgggcgacat
     2701 catcaacgag gtggacgaac gcggcgagcc gatcgtcggt gacacgctgc tgatgatcat
     2761 gaacgcgcac tgggagaagc tggacttcac gctgccggct gcgcgggtcg agcacgtgtg
     2821 ggagacgatg ttggacacgt cggacccgga cctgccgctg cgcgtctgtc gggcgggcga
     2881 aaactacccg ctgctgggcc ggtcgctggc gctgctgcgc acgtgcaaac cggaggaaac
     2941 cggcgcggtg tcgccggcgc agatcgagac gttgcggcgg agcgcggagc gcgccaatca
     3001 gccgtcgccg aaggaaccac cgctgctgcg ttagtccctc ataacaacgt gtagatgacg
     3061 tttatgatgg ttcgagaatc agtgcaggaa caggttgcac cgccggtagc gagcaatcgg
     3121 cgcatgccga ttggcgcgga ggcgctgccg ggcggcggcg tgagcttccg cgtttgggcg
     3181 ccggggcatc gcgacgtctc ggtcgtgttt gaagatcgat cgatcgccga gcttccgctg
     3241 gcgccggaat ccgacggcta cttcgccggt gtcagcgctg aggcgggcgc gggtagccgt
     3301 tatcgctatc gcctggatgg cgaggaaaat ttctatcccg acccggcgtc gcgctcccag
     3361 ccggatggcc cgcacgggcc gtcgcaggtg atcgatccaa ccgccttcca gtggaccgac
     3421 gatgactggc ccggcgtcgg cgccgccggt caggtgctgt acgagatgca cgttggcacg
     3481 ttcaccaccg aaggcacctg ggccgccgcc gaacgcgagc tgccggcgct gaaagagctg
     3541 ggcgtgacgt gcctggaagt gatgccggtg gcggacttcg ccggcacgtt cggctgggga
     3601 tacgacggcg tcaacctctt cgccccgacc cggttgtacg gctcgcccga tgacttccgc
     3661 cgattcgtcg atcgcgccca ccgcgtgggc atcggcgtca tcctcgatct cgtctacaac
     3721 cacctcggcc ccgatgggaa ctacctcacg aagttcaccc ccgactattt caacgccgag
     3781 cacaagaccg actggggcga tgcgctgaac ttcgacggcc gcaacagcgg cggggtgcgc
     3841 gagctttacc tttcgaatgc ccgctactgg atcgaggaat ttcatctcga cggttttcgc
     3901 ttcgacgcga cgcaggcgat cgtcgatagc tcatcgcggc atatcctggc cgagatcacc
     3961 gagacgtcgc ggcgagccgc gggcaaacgc agcgtctacc tggtcaacga gaacgaaccg
     4021 cagcacacgc ggttggttgg gcccgtcgac gccggtggat acgggatgga cgcgctgtgg
     4081 aacgacgatt tccaccacag cgcgatggtg gcgttgtcgg gtcacaatga ggcgtactac
     4141 agcgattatc tgggcaaacc gcaggagctg ctggcggcgg cgaagtgggg gta
//
LOCUS       DATIMH010000165         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4080528, whole genome shotgun sequence.
ACCESSION   DATIMH010000165 DATIMH010000000
VERSION     DATIMH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4080528"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2071
                     /locus_tag="VLI90_02770"
     CDS             <1..>2071
                     /locus_tag="VLI90_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13154.1"
                     /translation="VRDLKLILNSAFATADFHGAGIANLAGDLKGQLQKVQSELGQFV
                     DFGGKQYAGDFDVAIANNGDLTSPGGTAHASTTATLTNLKLGDDVNQPWVQAAFKADL
                     HRGNPEFVDTVKNILVTLQSGNPQSPTVDVYATANADLTGAAKADFHLSRANIDLVKA
                     RAEFTKMFPPDLFISGGNVAMTADGAYDGAAKRADITAVTLAARDATIQRQASDGRRV
                     DLLHNYTLNMNTAARADLSNGMAVQVPKLSVTDSQQMQLNVTDVDLQLSKPTSPGQPA
                     AEQNPLDKLRSAKLALLVPDLKQLQALANTFSQSSAPAPSPVDPKAAPPTTQPLPPLE
                     ITSGSLAMHADVSHSGSALALNVSDITGKDIAFHRGDVAYQVKPITMQLAASVEPGDG
                     KTTTKPIQKIQVTQLSGNLGVATLSMPTPIVVTGLDAASPSANGGIKIDGHLQDVTQL
                     LAALQAKTADAYPYRGDFAVTENIGSSASGINVNGGVNVAKFQVLKGNDVQFAEDQLV
                     LANDVSMASDYNSVAIKNVSANMQSSGALNVALRNGTVDDLKSARRMNLGVDLQYDLA
                     KLWPIIHPMLLTPGQEDQYKDLKLAGQFKKVIVLKGQYPTDVPMQQAIRQLSADADLA
                     VGLFDYNGMHIENLDVPISLRDGKASTIYADKPTGQNAAPPAVANQGKLDLSNITIDL
                     TQDPPPRG"
BASE COUNT          434 a          667 c          652 g          318 t
ORIGIN      
        1 atgtgcgcga cctgaagctg atcctcaaca gcgctttcgc caccgctgac ttccacggcg
       61 ccggcatcgc caacctcgcc ggggatctga aggggcagct tcaaaaggtg cagtcggagc
      121 tgggccagtt cgtcgatttc ggcggcaagc aatacgccgg tgatttcgat gtcgcaatcg
      181 ccaacaacgg cgatctcacg tcccccggcg gcaccgcgca cgccagcacc accgcgacgc
      241 tgacgaacct caagctcggc gacgacgtga accagccctg ggtgcaggcg gcgttcaagg
      301 ccgatcttca ccgcgggaat ccggagttcg tcgatacggt gaagaacatt ctcgtcactt
      361 tgcagagcgg caacccgcaa tcgccgacgg tggatgttta cgcgacggcg aacgctgatc
      421 tgaccggcgc tgccaaggcg gatttccatc tgagccgggc gaacatcgac ctggtcaaag
      481 cgcgggcgga gttcacaaag atgttcccgc ccgatctgtt tatctctggc ggaaacgtcg
      541 cgatgaccgc cgacggcgcg tacgacggcg cggcgaagcg ggcggacatc accgccgtca
      601 cgctggcggc gcgggacgcg acgattcagc ggcaggcgag cgacggccgg cgcgttgatc
      661 tgctgcacaa ctacacgctg aacatgaaca ccgccgcccg cgccgatctg tccaacggca
      721 tggccgtgca ggtgccgaag ctgagcgtga ccgattcgca gcagatgcag ctgaacgtga
      781 cggatgtgga cctgcagttg tcgaagccga cgtcgccggg tcaaccggcg gcggagcaga
      841 acccgttgga caagctgcgc tcggcgaagc tggcgctgtt ggtgccggac ctgaagcaat
      901 tgcaggcatt ggccaacacg ttcagccaaa gcagcgcgcc cgcgccgtcg ccggttgatc
      961 cgaaggccgc gccaccgacg acgcagccgc tgccgccgct ggaaatcacg agtggatcgc
     1021 tggcgatgca cgcggacgtt tcgcacagcg gctccgcgct tgcgctgaac gtctccgaca
     1081 tcaccggcaa agacatcgcc tttcaccgtg gcgatgtcgc gtatcaggtg aagccgatca
     1141 cgatgcagct ggccgcttcg gtcgagccgg gcgatgggaa aacgacgacc aagccgatcc
     1201 agaagattca ggtgacgcag ctcagcggca acctcggcgt ggcgacgctg tcgatgccga
     1261 cgccgatcgt cgtcaccggt ctcgacgccg cgtcgcccag cgccaatggc gggatcaaga
     1321 tcgacggcca cctgcaggac gtcacgcagc tgttggcggc attgcaggcc aagacggcgg
     1381 atgcatatcc gtatcgcggc gacttcgccg tcaccgaaaa catcggttcc agcgcaagcg
     1441 gcatcaacgt caacggcggc gtgaacgtcg ccaagttcca ggtgctcaag ggcaacgacg
     1501 tgcagttcgc cgaggaccag ctggtgctgg cgaacgatgt atcgatggcg tcggactaca
     1561 actcggtcgc gatcaagaac gtctcggcca acatgcaaag cagcggcgcg ctgaacgtcg
     1621 cgctgcgcaa cggcaccgtc gatgacttga agagcgcacg gcgaatgaac ctcggcgtcg
     1681 acctgcaata tgacctggcc aagctttggc cgatcatcca cccgatgctg ctgacgccgg
     1741 ggcaggagga tcagtacaag gacctcaaat tagcagggca gttcaagaaa gtgatcgtgc
     1801 tcaaggggca gtacccgacg gacgtgccga tgcagcaggc gattcgccag ctgtccgccg
     1861 acgcggatct ggctgtcggg ctcttcgatt acaacgggat gcacatcgag aacctcgacg
     1921 tgccgatttc attgcgcgac ggcaaagcgt ccacgattta cgccgataaa ccaaccggcc
     1981 aaaacgccgc gccacccgcg gtcgccaatc aaggcaagct cgatctcagc aacatcacga
     2041 tcgacctcac gcaggacccg ccgccgcgcg g
//
LOCUS       DATIMH010000166         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4368930, whole genome shotgun sequence.
ACCESSION   DATIMH010000166 DATIMH010000000
VERSION     DATIMH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4368930"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1293)
                     /locus_tag="VLI90_02775"
     CDS             complement(202..1293)
                     /locus_tag="VLI90_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HSV13155.1"
                     /translation="MPASSPVIVRHSRRRRLRVALLYTQAILLEFRGTLIALAILLII
                     GAALHLITPQPQLSNGRLPFGTAVYAGWMALLAQPQFAPPQPGYLIFIYSIYPLCGFI
                     LIGEGVIRLAQLMMSRRRGEKEWMIVMASTYRDHVILCGLGRLGIRVLEQLVTAGVAV
                     VALEKDPLSAFIDQARTMGVPVLLRDMKEDQALIDAGIVYADAIISCSNDDMANLEVA
                     LDARRMNPKIRVVLRMFEQAVASKIAGALTIDAAFSASSLAAPVVAAMSLKTKVLSTM
                     MIAGVPHVAAEVTVAADSHLAQKRIGELEVGYGARVLARTPAGAATQDSPTPGTALTA
                     GDTLVVYAPASQMPTIAAAAGARDLVARI"
     gene            1812..2936
                     /gene="dnaN"
                     /locus_tag="VLI90_02780"
     CDS             1812..2936
                     /gene="dnaN"
                     /locus_tag="VLI90_02780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HSV13156.1"
                     /translation="MKVICNRGALLEALTVTSNVVQARTPKPVLQCVKVVAADDRLTI
                     AATDLEVAIRYSDSQVQIEQPGETLVPADKFRDIVRESVDDTLSIEVSTEKDGAGGTF
                     ANIRGQDSHFKIFTQKASDFPPVPDFEGEADFELPGGQLKQLIGQTIFAAAKEPGRYN
                     FNGVLTVTKGKKINLISTDGRRLAMAKGDLATDRLEKDGSKVIIPSKALNLIDKLIDD
                     PEEMVGFQVRENQVIFHTSSATLTSNLVEGQFPPYEDVIPKDTDKKMSAATADFLSAI
                     RRAALLTTEESRGVKMQFSKKGLVLTSRSPEAGEATINFACKYEGSDVEIGFNPAFLT
                     EALRVVDSDEISLEMTAPNRPGLLKGGANFLYVIMPVNLQ"
     gene            3070..3447
                     /locus_tag="VLI90_02785"
     CDS             3070..3447
                     /locus_tag="VLI90_02785"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HSV13157.1"
                     /translation="MALQRLQRVKQRDTTVGAALGPAMIAFFKQSVTKRQTKLEKIAS
                     CWGSLVPEMFNDHCALESWNKGTLTVIVDSSSHLYELKQLLLAGLQQQLLLACKSTGL
                     RKIALKPGRWYDGGDEERRPRFD"
     gene            complement(3474..>3679)
                     /locus_tag="VLI90_02790"
     CDS             complement(3474..>3679)
                     /locus_tag="VLI90_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13158.1"
                     /translation="GMPFIADHPNIAGASRQSATTGKQTPPATIVPPRKHYLWDIADI
                     TPAITLGERTKMRIFWICRNCQS"
BASE COUNT          731 a         1066 c         1235 g          647 t
ORIGIN      
        1 gcaggccgac ggccgtcgtc ggcagggccc agagatattt gacgggacgg tggagattca
       61 tcggatggtg atttcccctt tgatagccgg gggcggaagc cccggtcttt tttcccgttc
      121 gaacggaccg gggcttccgc ccccggctat taaagagtct agagaaattc tagcgggcga
      181 accattcgca cgatgcgcaa atcatatgcg cgcgacgagg tcgcgcgcac cggccgccgc
      241 ggcgatcgtc ggcatctgcg atgccggcgc gtaaaccacc agcgtatccc ccgcggtcag
      301 cgcggtgccg ggcgtcgggc tatcctgcgt ggcagcgcct gccggcgtgc gggccaggac
      361 cctcgcgccg taaccgactt ccagttcgcc gatgcgcttt tgcgctaagt gactgtcggc
      421 ggcgaccgtc acctccgccg ccacgtgcgg gacgccggcg atcatcatcg tgctgagcac
      481 tttcgtcttc agcgacatcg cggcgacgac tggggccgcc agcgacgacg cgctgaaggc
      541 ggcgtcgatc gtcagcgcgc cggcgatctt cgacgccacc gcttgctcga acatgcgcag
      601 cacgacgcgg atcttcgggt tcatcctgcg cgcgtccagc gccacctcca ggttggccat
      661 gtcatcgttg gagcaactga tgatcgcgtc ggcgtagacg atgccggcgt cgatcagcgc
      721 ctggtcttcc ttcatgtcgc gcagcagcac gggcacgccc atcgtgcgcg cctgatcgat
      781 gaaggcgctg agcgggtcct tctccagcgc gacgactgcg acgccggcgg tgacgagctg
      841 ttcgagcacg cggatgccca ggcggcccag gccgcagagg atgacgtggt cgcgataagt
      901 ggaagccatg acgatcatcc attccttctc accgcggcgg cgtgacatca tcagctgggc
      961 caggcggatg acgccttcgc cgatcaggat gaagccacac agcggataga tgctgtagat
     1021 aaagatgagg taacccggtt gcggcggggc gaactgcggc tgcgcgagca gcgccatcca
     1081 accggcgtac accgcggtgc cgaacggcag gcggccattg cttaactgcg gctgcggggt
     1141 gatgaggtgt agagctgcgc cgatgatcaa caagattgcc agcgcgatga gggtgccgcg
     1201 aaattcgagt aggatggctt gcgtgtagag cagcgcgacg cgcagccggc gacgacggga
     1261 atgccgcacg atgacaggcg agctggcggg catgcgagat gcgatggaga gtaatcgaaa
     1321 gcgcgattcg atgcgtagcg gtagccccgc aggggcacat gttttcctct ggaaggaaga
     1381 aacacgtgcc cctgcggggc taccgctacg cagggcgcta cggacggcgc tacgtggcgg
     1441 atcggcgctt tcatcaggcc tcgcgcacaa ccggtggaaa acttggcggc gcttctcgcg
     1501 ggcgcagcgc gagcggttcg atccggcaac acccgcggcc ttcatcgcgc ggggcctgtc
     1561 gcgccgggcc gcgccgggca tcgagtcgtc atcacagaac tgaacaggtt atccacggcc
     1621 ttcgtccgat aatgccactc gtaacgttcg acgtcgattg cgcttatcct gtcgtcgccg
     1681 aagttttcca caatgcgcca ggcatcatta cgactgctac tatctttaaa gtttgggtat
     1741 aagaatagag acagtaaagt acgccccgct tgatcgggct cacttaacgg aactgggaaa
     1801 ggccttgaaa catgaaagtg atctgtaacc gcggcgcgct gctggaagcg ctgaccgtga
     1861 cgagcaacgt cgtgcaggcg cgcacgccca aaccggtgct gcaatgcgtg aaggtggtgg
     1921 cggcggatga tcgtctgacg atcgcagcga cggacctgga agtcgccatt cgctacagcg
     1981 attcgcaggt gcagatcgaa cagccgggcg aaacgctggt gccggccgac aagttccgcg
     2041 acatcgtgcg cgagagcgtg gacgacacgc tgtcgatcga agtatcgacg gagaaagacg
     2101 gcgccggcgg gacgttcgcg aacatccgcg ggcaggattc gcatttcaag atcttcacgc
     2161 agaaggcgag tgactttcca ccggtgccgg actttgaggg cgaagcggat ttcgaattgc
     2221 cgggcgggca gctgaagcag ctgatcgggc agaccatctt cgccgcggcg aaggagccgg
     2281 ggcggtacaa tttcaacggc gtgctgacgg tgacgaaggg gaagaagatc aacctgatca
     2341 gcaccgacgg tcgccgcctg gcgatggcca agggcgacct ggcgacggac cggctggaga
     2401 aggacgggtc gaaggtcatc atcccgagca aggctttgaa tctgatcgac aagttgatcg
     2461 acgacccgga agagatggtc ggctttcagg tgcgcgagaa ccaggtgatc ttccacacca
     2521 gcagcgcgac gctgacgagc aatctcgtcg aaggccagtt cccgccgtac gaagatgtca
     2581 tcccgaaaga cacggacaaa aagatgagcg cggcgacggc ggattttctg tcggccatcc
     2641 ggcgcgccgc gctgctgacg accgaagaaa gccgcggcgt gaagatgcag ttctcgaaga
     2701 aggggctggt actgacgagc cggtcacccg aggcgggcga ggcgacgatc aacttcgcgt
     2761 gcaagtacga aggcagcgat gtggagatcg ggttcaatcc ggcgttcctg accgaggcgc
     2821 tgcgggttgt ggacagcgat gagatttcgc tggagatgac ggcgccgaat cgcccggggc
     2881 tgctgaaggg cggggcgaat tttctgtacg tgatcatgcc ggtgaatttg cagtagtgct
     2941 taacagccgc gggcgacagc cagcggggtc tttcgagcac cagccgggct gagattccaa
     3001 acccccgctg gctttcgcct gcggctatca gggtgagctt tgatggggat ggttcctcac
     3061 caatccgact tggcacttca gcgtctgcag cgcgtcaagc agcgcgacac gaccgtcggc
     3121 gccgcgctcg gcccggcgat gatcgcgttt ttcaaacagt ccgtcaccaa gcggcagacg
     3181 aagctggaga agatcgccag ctgttggggc agcctggtgc cggaaatgtt caacgatcac
     3241 tgcgcgctgg agtcgtggaa caagggaacg ctcaccgtca tcgtcgatag ttcatcgcac
     3301 ttgtacgaat tgaagcaact cctgctcgcg gggctgcagc agcaactgct gttggcgtgc
     3361 aagtcaacgg gactgcggaa gatcgcgctc aagccgggcc ggtggtacga cggcggcgat
     3421 gaagagcggc ggccgcggtt cgattgagcg cggctgactt ctcgtaagca gcctcagctt
     3481 tggcagttac ggcaaatcca aaagatccgc attttcgtcc gctcccctaa cgttatcgcc
     3541 ggggtaatgt ccgctatatc ccagaggtag tgtttacggg gcggaacgat cgttgcaggc
     3601 ggcgtttgct tgccggtggt tgccgactga cgcgaagcgc ctgcaatatt gggatggtct
     3661 gcaatgaagg gcataccga
//
LOCUS       DATIMH010000167         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3503945, whole genome shotgun sequence.
ACCESSION   DATIMH010000167 DATIMH010000000
VERSION     DATIMH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3503945"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..1492)
                     /gene="trpS"
                     /locus_tag="VLI90_02795"
                     /pseudo
     CDS             complement(392..1492)
                     /gene="trpS"
                     /locus_tag="VLI90_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
     gene            complement(1557..2693)
                     /locus_tag="VLI90_02800"
     CDS             complement(1557..2693)
                     /locus_tag="VLI90_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSV13159.1"
                     /translation="MPASREQIAQATAKRLASAASSLATIKAFIGLDGFVDEIIAVVD
                     KRQSFQSYDPVKTIAHLGQKILNAAGQSSNYELIVKQMKLGGNGPIMANALASFGLAV
                     TYVGSLGYPALHPVFQEMAGRAKVISIADAGHTDALEFEDGKLMLGKITSMNEVTWAN
                     LVERAGGTAELRRMVDESALFGFVNWTMLPHMSDIWDRLRTEIMPSVSKKPRKFFVDL
                     CDPEKRTHADIERAMRILTQLQESVDVILGLNLKEALAILDVLGLAAPTDPEATIEET
                     AKAIRQLMKIYCCVVHPRSGAAAATSSASAKFAGPFVQQPRISTGAGDHFNAGFALGQ
                     VLGLELDQSLCCGVGTSGYYVRTGISPTAKQLAEFVETLPAPQS"
BASE COUNT          512 a          905 c          918 g          576 t
ORIGIN      
        1 aacttcctgt cattcggttt aacgcgttct gtctcacgaa gcggattcga cggcaactgc
       61 agcttagcga gagacttcaa tgagacgacc ggccgagtag cccggttcgc gaaaagcgcg
      121 gccaaacccc cacgaatcgg cggcggttgc cgaatgataa cagagatcgg aaaggcgggg
      181 tagggaaggt tggggttttc cgggggtggg gttatgggac gcgcgactcc cgttgatctg
      241 ccgcgtagca gtcgccccga aggggcacgt tccggtttcc ttcaacgatg aaagacggat
      301 caaaccacga aggacatgaa gggtacgcag cacagccgct cgatctcgcg cgcggcgcag
      361 gggtgagcct gcgcgctcga tgcgaagcac tctacgtcaa cgccagcgtt cgcgagaaat
      421 aatcctgccg catcgcttgc ttggcgaggg cgagggtttg ctccgcttgg acgttggccc
      481 gtttcgtccc cgctcgcaac gcgtccatca cgtcgtcggg gcgctgctcg aaatgttttc
      541 tgcgcgagcg gatcggctcg atgatcgtgt tcagcacctc gaccagcttg cgcttgatga
      601 ccacgtcgcc gaccttgccg ttcttgtacg cctcgcgctg ctcgtcaacc caggcggtgt
      661 cggggttgaa cgtttcgtga aacgcccaga ggggattctt ggtcgggtcg gtctcgccgg
      721 ggtcagtggc gcgaagtcgc ttggggtccg tgtacatccc catcactttt ttctgcaccg
      781 tgtcggcgtc gtcggccagg aagatcgcgt tgttcagcga cttgctcatc ttgagcaatt
      841 cgccgctggg accgggcgcg ccggtgccga cgagccgctt gacgcggccg agctttgctt
      901 cgatgacggg gaacagcccg ccggctttga cgttgtcctt atcaggcgtc tgcgggtcca
      961 cgctgcggta catctgattg aaccggcggg cgaccgtgcg cgccagctca agatgcggca
     1021 gttggtcttc accgaccggc accagctgcg ggcggaacgc gaggatgtcg gcaacttgcc
     1081 cgacgaggta catcggaaag ccgaacggat aattgtcgcc cagcccctag tcgcggatct
     1141 cgtccttgat cgtgggattt ttcatcacga cgttgaacgg gatcagcatt gcgaaaaaga
     1201 acgtcagctc gtcgatcgcg ggcacttcgc tctggatgaa catcgcgctc ttggccggat
     1261 cgatgccgac ggcgagccaa tcggtggcga tgtcgatcac actttggcgg atgtcggcag
     1321 gcttgtcggc gcgcgtgctg aacgcgtgct tgttggcgat caggaagtag cactcgtact
     1381 gatcctgcag cgcgagccgc gtttcaacgc tgccgacgta gtgaccgaga tggagttttc
     1441 cggtgggcgt atcgccggtg aggatgcggg gtttggtgga cggttcggac atcgcgaatg
     1501 ctcttttggt gaacaggccg acccgattaa gcgggtcggg ccggtggggc ccgcctttaa
     1561 ctctgcggcg ccggcagcgt ctcgacaaat tccgccaact gcttggccgt cgggctgatt
     1621 ccggtgcgga cgtaataccc gctcgtgccc acgccgcagc acaggctttg atccagttcc
     1681 agccccagca cctgccccag cgcgaagccc gcgttaaaat gatcgcccgc gccggtgctg
     1741 atccgcggct gctgcacgaa tgggcccgcg aacttcgcgc tcgccgaact cgtcgccgcc
     1801 gccgccccgc tgcgcggatg tacgacgcag caatagatct tcatcagctg gcgaatcgcc
     1861 ttggccgtct cttcaatcgt cgcctccgga tccgtcggcg ccgctaatcc cagcacgtcc
     1921 aggatcgcca gcgcttcctt caggttcagg ccaaggatca catccaccga ctcctgcagc
     1981 tgcgtcagga tccgcatcgc acgctcgatg tccgcgtgcg tgcgcttttc cggatcgcac
     2041 agatcgacga aaaacttccg cggcttcttc gatacgctcg gcatgatctc cgtgcgcagg
     2101 cggtcccaga tgtcgctcat gtgcggcagc atcgtccagt tgacgaagcc gaacagggcg
     2161 gattcgtcca ccatccgccg cagttctgcg gtgccgccgg cgcgctcgac gaggttcgcc
     2221 cacgtcacct cgttcatcga cgtgatcttg cccagcatca gcttgccgtc ttcgaactcc
     2281 agcgcatcgg tgtgcccggc gtcggcgatg ctgatcacct tcgcccgccc cgccatctcc
     2341 tggaagaccg gatgcagcgc ggggtaaccg aggctgccga cgtacgtgac ggccaacccg
     2401 aagctggcca gcgcgttggc catgatcggc ccgttcccgc cgagcttcat ctgcttgacg
     2461 atcagttcgt aattgctgga ctgccccgcc gcgttcagga tcttctggcc caggtgcgcg
     2521 atcgtcttga ccgggtcgta cgactggaac gactgccgct tatcgacgac ggcgatgatt
     2581 tcatcgacga acccgtcaag gccgatgaag gctttgatgg tcgccagcga tgaagccgcc
     2641 gacgcgagcc gcttggccgt cgcctgtgca atttgctccc gcgatgcagg catggatgtc
     2701 ttctccgcag ctcagaaggt ttcgcgcgcg ccgcacgcac acatggagcg cgcgctgcgg
     2761 tttatatccg cgaatgggtg aaacgtgaaa tcgtgcgttg cgacggggga aagacaatgc
     2821 aacggccgag ccgccgagcg cgccgaggcg gacgccgagc gaacaaaagc gagaagaggg
     2881 tggaaccaca gatttcacag gttacacaga t
//
LOCUS       DATIMH010000168         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_904766, whole genome shotgun sequence.
ACCESSION   DATIMH010000168 DATIMH010000000
VERSION     DATIMH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904766"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VLI90_02805"
     CDS             <1..583
                     /locus_tag="VLI90_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13160.1"
                     /translation="TRAVQRALAGLPVDVITIGIRGCRVPIDAEHPLPAEAVVILAGM
                     GGALDPSLKVGDAVVDDPAGLLPASIDGVPRGMIHGADQIIATPAEKAALHGRTGALA
                     VDMESGVVRRLSGRVINVRAISDSADEVLDPRVIGLVDDVGRARPMKIGAALVKSPGL
                     ARYLARLGKNSKIACEALGGAVRRIVEALLKSS"
     gene            854..2140
                     /locus_tag="VLI90_02810"
     CDS             854..2140
                     /locus_tag="VLI90_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13161.1"
                     /translation="MMDQLGIRKLRPGPDPKNQSTFDEATANRYADSMPDVLRMKDGT
                     RVTSAAQWPARRAEIVEDFEREVYGRIPPDVPAVTWRVTASTRGLSGQTPTLTRTLVG
                     HVDNSAFPKLSVDIQARVTVPERSEQPVPVMVEISGFGFASSRPTTVGSSTRRRPTSA
                     TGPSWQDQAIAHGWGYAAINPASVQPDNNHLTSGIIGLTNKGKPRTPEQWGALRAWQW
                     GVSRLIDCFEQHPEWSVDAKRIGVEGLSRYGKAALVTEAFEPRIAVGLIGSSGEGGAK
                     LHRHIFGEAVENLAGGEYYWMAGNFIKYGASDPPKTVADLPVDSHELIALCAPRPCFI
                     SHGVPEHGDANWIDARGSFMAAVLASPVYELLGAQGLGTHGDYLTEKMPPTLTLMGGQ
                     LAWRQHEGGHDVTPNWPAFFDWVPRYIPTRVAPTSR"
     gene            2404..>3716
                     /locus_tag="VLI90_02815"
     CDS             2404..>3716
                     /locus_tag="VLI90_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV13162.1"
                     /translation="MSDHGRRELNHMALGEIARRHAAMVYAIARRLVGDPHLADDVTQ
                     AVFILLLQKAGNLRNEAHLVGWLHTTTRHAAANARKMEQRRKHYEARAGQRAVVERRE
                     QRSRENHWAELSPVIDEALAGLGERDRQAILLRYICGKGVADVAAATNVTPAAAKKRL
                     RRGLEKLRLVLSRRGVVMPATLLASTLMLSRNVVHGATGELPNSILNLLNGSSRAAAG
                     SSTASTIAARTGAHVARWQTMAAAAAIIIAAALAICFALDGKAPLEQTAQADPTSAKP
                     IAPPAAPAPPVTSEPTANITAVGQLVALPGQVVGSPTPVDLDGDGEPEIVVPYMGMVS
                     GSQGSMTGDVPLTNGREPDLAAYVGAFHLDGSAVPGFPVVVMSAEHHKQAGPNEFPNW
                     WLGTPAVRGAHNGQSGVIVIGHPSGPAKADRGISIIRGDGSHLEVA"
BASE COUNT          685 a         1140 c         1287 g          604 t
ORIGIN      
        1 aacccgcgcg gtacagcgcg cgttagcggg gttgccggtg gacgtgatca ccatcggcat
       61 tcgggggtgc cgagtgccaa tagatgccga gcacccattg cccgcggaag cggtggtgat
      121 cctggcgggg atgggcggtg cgctggatcc atcactgaag gtcggcgacg ccgtagtgga
      181 tgaccccgcc gggcttttgc cggcgtcgat cgacggcgtc cctcgcggga tgattcacgg
      241 tgcggatcag atcatcgcga cgccggcgga gaaggcggcg ttgcatggcc ggaccggcgc
      301 cttggcggtg gacatggagt ccggcgtcgt ccggcggttg agtgggcgcg tgatcaatgt
      361 tcgcgcgatc agcgattcag cggacgaggt gctggacccg cgcgtcatcg ggctggtgga
      421 tgacgtgggc cgagcgcggc cgatgaagat cggcgccgcg ttggtaaagt cgccggggct
      481 ggcgcggtat cttgcgcgac tgggaaagaa ttcgaagatc gcgtgcgaag cgctcggagg
      541 cgcggtgcga aggatagttg aagcgctgct gaagtcgtcg tgaccgatcg caagcgcgca
      601 acacgcgccg tcgtttcgcc gccgcggcct cgcggcgcgg atgtcaaaaa gcgccgcgag
      661 tccgaacgcg aattaaaaaa agcgccgcga ggccgcggcg gttaaacgcg gtggtcttgt
      721 cgtcacagag ggatacatga tgacctcctg cttcggatct cggttgctgc tcgtcgcatc
      781 cacgttcgcg ttcttccttg cgacgccaca catatctttc gcccagacgc agccattcga
      841 cgaccatcaa aacatgatgg atcaactcgg cattcggaaa ctccggcccg ggccggatcc
      901 gaagaatcag tccacattcg acgaggcgac cgccaatcga tacgccgaca gcatgccgga
      961 cgtgctgcgg atgaaggatg gcacgcgggt gacgtcggcg gcacagtggc ccgcgcggcg
     1021 cgctgagatc gtcgaggatt tcgagcgcga ggtgtacggc cgaatcccac cggacgtgcc
     1081 ggcggtgacg tggcgtgtga ccgccagcac ccgcgggttg agtggacaga cgccaacgct
     1141 gacgcgcacg ctggtcggtc acgtcgataa ttcggcgttc ccgaagctgt cggtggacat
     1201 ccaggcaagg gtgaccgtgc cggaacgctc cgagcaaccg gtgccggtga tggtggagat
     1261 cagcggcttc ggtttcgcgt cttcgcgacc cacaacggtg ggaagttcca ccagacgacg
     1321 gcccaccagc gccaccgggc cgtcgtggca agaccaggcg atcgcgcacg gctggggtta
     1381 cgccgcgatc aatcctgcca gcgttcagcc cgacaacaac cacttgacca gcggcatcat
     1441 cggcctgacg aacaagggca agccgcgcac gccggagcag tggggagcgc tgcgcgcgtg
     1501 gcaatggggc gtgagccgat tgatcgactg cttcgagcag catccggaat ggagcgtcga
     1561 tgcgaagcgg atcggcgtcg aaggattgtc gcgctatggc aaagccgcgc tggtgaccga
     1621 ggcgttcgag ccgcgcatcg cggtagggct gatcggttcc tcgggcgaag gcggcgcgaa
     1681 gctgcatcgg cacatctttg gtgaagccgt cgaaaacctc gccggcggcg agtactactg
     1741 gatggccggg aacttcatca agtatggcgc atctgacccg ccgaaaaccg tggcggacct
     1801 gccggtcgat tcgcacgagc tgatcgcgct gtgcgccccg cggccgtgct tcatcagcca
     1861 cggcgtgccc gagcacggcg acgccaactg gatcgacgcc cgcggcagct tcatggccgc
     1921 cgtgctggcg agtcccgtct atgagttgct cggcgcccaa ggcctcggca cgcacggcga
     1981 ttatctcacc gaaaagatgc cgcccacgct cacgctcatg ggcgggcagc tggcttggcg
     2041 acaacatgaa ggcggccacg acgtcacacc caactggccg gcgttcttcg attgggtgcc
     2101 gcgatatatt ccgacgcggg tggcgccgac gagccgctga cggagttcaa agctcaaatg
     2161 caaccgccga gggcgccgag cacgccgagg cagactccga gaagatcttc agagaagaag
     2221 tttcaccaca gatttcacag attacacgga ttgaagaagg cccctcctta atctgtgaaa
     2281 tctgtggtta aaaactcttc ttcattctct cctcggcgtc tgggtggttt cattcatcgc
     2341 taaactggaa tcagcaccac ttttctggtg tccccgccgt aggcggagcg tacattatcc
     2401 ggcatgtccg accacgggcg gcgagaactg aatcacatgg cgcttggcga gattgcccgc
     2461 cggcacgcgg cgatggttta tgccatcgcc cggcggctcg ttggcgatcc gcacctggcc
     2521 gacgatgtga cgcaggcggt gttcatcctc ctgctgcaga aggcgggcaa cctgcgaaac
     2581 gaagcgcacc ttgtgggctg gctgcacacg acgacgcggc acgcggccgc caacgcacgg
     2641 aaaatggaac aacgccgtaa gcattacgaa gcgcgcgcgg ggcagcgggc agtcgtcgaa
     2701 cggcgcgagc aacggtcgcg cgaaaatcac tgggcggaac tgtcgccggt cattgacgag
     2761 gcgctggcgg gactgggcga gcgcgatcgc caggcgattt tgctgcgata catctgcggc
     2821 aaaggcgtgg ccgacgtggc ggcggcgacg aatgtcacgc cggcagcggc caagaagcga
     2881 ttgcggcgcg gattggaaaa gctgcgcctg gtgctgtccc gccgcggggt tgtgatgccg
     2941 gcgacgttgc tcgcatcgac actcatgctt tcgcgaaacg tcgtccacgg cgcgacgggc
     3001 gagcttccca attcaatctt gaacctgctg aacggatcga gccgcgcggc agccggttcg
     3061 tctacggcca gcaccatcgc cgcgcgaaca ggcgcgcacg tggcacgatg gcagaccatg
     3121 gccgcggcgg cggcgatcat catcgcggcg gcgctggcga tttgcttcgc gctcgatggc
     3181 aaagcgccgc ttgaacaaac ggcgcaggcg gatccgacca gcgccaaacc cattgcaccg
     3241 ccggcggcgc cggcgccgcc ggtgacgagc gagccgacgg cgaacatcac cgccgtcggc
     3301 cagttggtcg cattgcccgg gcaagtcgtg ggttcaccaa cgccggtcga tctggatggc
     3361 gacggtgagc cggagatcgt cgtgccgtat atgggcatgg taagcgggtc acagggttcg
     3421 atgaccgggg acgtgccgct gaccaacggg cgcgagccgg acctggcggc gtacgtcggc
     3481 gcgtttcacc tggatggatc ggcggtgccg ggatttcccg tagtcgtcat gtcggccgag
     3541 catcacaagc aggcggggcc gaacgagttt cccaactggt ggctgggcac ccccgccgtg
     3601 cgcggcgcgc acaacgggca gagcggcgtc atcgtcatcg gtcatcccag cggtccggcg
     3661 aaggcggacc gcggcatctc gatcatccgc ggcgacggca gtcacctgga ggtggc
//
LOCUS       DATIMH010000169         4882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3792634, whole genome shotgun sequence.
ACCESSION   DATIMH010000169 DATIMH010000000
VERSION     DATIMH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3792634"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VLI90_02820"
     CDS             <1..440
                     /locus_tag="VLI90_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSV13163.1"
                     /translation="RLAFLTGKIAARHGEHRPALVQVQRVFAELKAEMDSHMMKEERI
                     LFPLCRQLDTADALPSSHCGSVANPIEVMIREHDHAGDALARIRALTDTYTVPADACN
                     TYRALFDSLQQLERDMHQHVHKENNILFPKAIRQERLLSAASR"
     gene            complement(551..1036)
                     /locus_tag="VLI90_02825"
     CDS             complement(551..1036)
                     /locus_tag="VLI90_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSV13164.1"
                     /translation="MTAMFSQTAEYALRIVVYLASLDGEAPATIAEIATATRTPPGYL
                     AKVLRQLARANLIHSQRGPHGGSVLARDAGHITVYDVVQAVDPIQRITTCPLGIRAHG
                     VKLCPLHLRLDSAIAMVERAFKDSPISELIVPRRGGSRALCDEPAIAKRTKRTRSLPV
                     L"
     gene            1184..2275
                     /locus_tag="VLI90_02830"
     CDS             1184..2275
                     /locus_tag="VLI90_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13165.1"
                     /translation="MPRLKPEKIRAGCWGAAVTVLLAVMIVLGSRNLNHFDAALVGYT
                     FATLFATFGITYRYAMWLQRPPTRMYWRRGWQVFLNPRFLGRNSIELARRFFLEFVAN
                     RFIFHRGRTRWAAHWLIMWGCLLAAAITFPLVWGWIHFETVPGNITLYRTFVFGFPVQ
                     DFPADSLPAFIVFHGLVWASFLVIAGVMIAFRRRMIDHGAVAVQQFGEDILPLLLLFA
                     ISVTGLMLTVSYTWLRGYAYDFLAILHALTVIITLIWLPFGKFFHIFQRPAQLGVSFY
                     KDVGRTNEQALCRRCGQPFASAMMVRDLMTVERQLGFSYETDQDGVEHYQQVCPRCRR
                     ALFGLSQGAMWTKAIQRRSDGSHPIEAGV"
     gene            2244..2591
                     /locus_tag="VLI90_02835"
     CDS             2244..2591
                     /locus_tag="VLI90_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HSV13166.1"
                     /translation="MAAIRLKRAYEPPSADDGQRILVERLWPRGLTKAGAKIDEWMKD
                     VAPSPELRKWFAHDPAKWKQFQQRYWKELRHESDAVDQLRKKAKKRRVTLIYAARDEE
                     HNGALALKRFLEK"
     gene            2666..3268
                     /locus_tag="VLI90_02840"
     CDS             2666..3268
                     /locus_tag="VLI90_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSV13167.1"
                     /translation="MAVQLGARPEHSFDEPLGLLSDCHRRIEKFLDVMIRVLDRSDRG
                     NKPLALDERDALESALRYFKNAAPRHTQDEEQSLFPRLRASDDPAAHAALARIEALEE
                     DHRRADAMHDEVHQLCRRWLDSLAISAMEAERLNQLLHDLRDTYAGHIALEDSELFPL
                     AARVLKDADLKAVGAEMAQRRGLDANGPMSRPKCPSKNRS"
     gene            3305..>4882
                     /locus_tag="VLI90_02845"
     CDS             3305..>4882
                     /locus_tag="VLI90_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSV13168.1"
                     /translation="MATIPTDELKIINEFGPHLARMSGERLSTSIEPDRLVKTHCCFC
                     GQQCGIQLKVRGNEVIGFEPWEDFPFNRGMLCPKGVKRYLQGSHPDRLLTAIRRDPSA
                     PGGFTPMPYTDAIAKVASEIQRLQSTYGNGAIAVLGGASLTTEKTYLLGKFARVCLKT
                     PFIDYNGRLCMVSAGAANKKAFGIDRTTNPWSDMIGTGVIWVAGSNVAECSPITTNYI
                     WQAREMGAKVIVQDPRITPIARTCDLFLPVKPGRDAAVFAGVLNLMIEHGWIDRDFIE
                     QHTVGFEQVAEYCKQWTPARTAEVTGVPERSLRQAAELWGTAKSSFLLHARGIEHHSN
                     GVQNALGTINLVLASGRIGKSNSGYGTIVGQANGQGGREHGQKCDQLPGWRDISNPEH
                     RKYIAGVWGVDESSIPGPGVDAYELFRKIDRGEIKGLISICFNPKISLPDNTFIARAL
                     SKLEFYVAIDFFLNDTAHHADIVLPGSLQEEDEGTVTQVEGRVIKINKAVDCPGEARR
                     DWVILQDIARALNRTHGF"
BASE COUNT          918 a         1488 c         1607 g          869 t
ORIGIN      
        1 cgcgactggc gttcctgacg ggcaagattg ccgcccgcca tggagagcat cggccggcgc
       61 tggtccaggt gcagcgggtg ttcgcggagc tcaaggcgga gatggacagc cacatgatga
      121 aggaagagcg gatcctcttc cccctctgcc ggcagctgga cacggccgat gcgctgccga
      181 gttcgcactg cggcagcgtg gcgaatccga tcgaggtgat gatccgcgag cacgaccacg
      241 cgggcgatgc cctggcgcgc attcgtgcgc tgacggatac ctatacggtg cccgccgacg
      301 cgtgcaacac gtaccgtgcg ctgttcgatt cgctgcagca gttggaacgc gacatgcacc
      361 agcacgtgca caaggagaac aacatcctgt tccccaaagc gatccgccag gaacgattgc
      421 tgtcagcggc gtcgcgatga acgttcgcgc ggccgcctcg ctttagcgag tccggctttg
      481 ttcgcacgcg cgcggacggg aaaaagccca ctcgcttagg cgagtcggcc aatgatggga
      541 agcgcggcgt ttatagcacc ggtagtgatc gagtccgttt ggtgcgcttg gcaatcgccg
      601 gctcatcgca taaagcgcgg ctaccgccgc ggcgcggaac gatcagctcg gaaatgggcg
      661 aatccttgaa cgcccgctcg accatcgcga ttgcgctgtc gagccggagg tgcagcgggc
      721 agagcttgac gccgtgagca cggatgccca gcgggcaggt ggtgatgcgc tggatcgggt
      781 cgaccgcctg cacgacgtcg tacacggtga tgtgcccagc gtcgcgggcc agcaccgatc
      841 caccgtgcgg cccgcgctgc gagtggatta gattggcgcg cgccagctgc cgcaacactt
      901 tcgccaggta ccccggcggc gtgcgggtcg cggtggcgat ctccgcgatg gtcgccggcg
      961 cctccccgtc gaggctggcg aggtaaacga cgatgcgcag cgcgtattcg gcagtctgcg
     1021 agaacatggc ggtcatgata gcacattctg gatgtcaata tccagatcgc gcataaatcg
     1081 gttgggtcgc atctatattt gctgtcgcgg atttgatatt gcggtaaggt ggagtgggct
     1141 ggaagatggg acccatggcg ggcgaggcag cgaggagcgg attatgccgc ggttgaaacc
     1201 ggagaagatt cgtgccggct gctggggcgc ggcggtgacg gtcctgctgg cggtcatgat
     1261 cgtgctgggc tcgcgcaacc tgaaccactt cgacgccgcc ctcgtcggtt acaccttcgc
     1321 cacgctcttc gcgacgttcg gcatcaccta tcgctacgcg atgtggctgc agcgcccgcc
     1381 gacgcgcatg tactggcggc gcggctggca ggtcttcctc aatccgcgat ttctcggccg
     1441 aaacagcatc gaactcgccc ggcggttctt cctcgaattc gtcgccaacc gcttcatctt
     1501 ccaccgcggc cgcacgcgct gggcggcgca ctggttgatc atgtggggct gcctgctggc
     1561 cgccgccatc acgttcccgc tggtgtgggg gtggatccat ttcgagaccg tgcccggcaa
     1621 catcacgctc tatcgcacgt tcgtgttcgg cttcccggtg caggatttcc ccgcggattc
     1681 cttgccggcg ttcatcgtat ttcacgggct ggtctgggcg tcgttcctgg tgatcgccgg
     1741 cgtgatgatc gcgtttcgcc gccgaatgat cgaccacggc gcggtcgcgg tgcagcagtt
     1801 cggtgaggac atcctcccgc tgctgctgct gttcgcgatc agcgtgaccg gattgatgct
     1861 gaccgtcagc tacacctggc tgcgcggcta cgcgtacgac ttcctcgcga tcctgcacgc
     1921 gctgaccgtc atcatcacgc tgatctggct gccgttcggt aagttcttcc acatattcca
     1981 gcgaccggcg cagctgggcg tgagcttcta caaagatgtt ggccggacga acgaacaagc
     2041 gctgtgccgg cggtgcggac agccgtttgc ttcagcgatg atggtgcgcg acctgatgac
     2101 ggtcgagcgg caactgggct tcagctacga aaccgaccag gacggcgtcg agcattacca
     2161 gcaggtctgc ccgcggtgcc ggcgggcgct gttcggcttg tcgcaaggcg cgatgtggac
     2221 caaggcaatc cagcggagga gcgatggcag ccatccgatt gaagcgggcg tatgagccgc
     2281 cgtcggctga cgatggtcag cgcatcctcg tcgagcgttt gtggccgcgc ggattgacca
     2341 aggccggcgc gaagatcgac gaatggatga aggacgtcgc gccgagcccc gagcttcgca
     2401 aatggttcgc ccacgacccg gcgaagtgga agcagttcca gcagcggtat tggaaggagc
     2461 tgcggcatga atccgacgcg gtcgatcagc tgcggaagaa ggcgaagaag aggcgggtga
     2521 cgttgatcta tgccgcgcgc gatgaggagc acaacggcgc attggcgctg aagaggttcc
     2581 tcgaaaagta gcacgggcgt ctcgcccgtg cctgagcgcc tcggcacggg cgagacgccc
     2641 gtgctacgga agatggagtg taaccatggc tgtgcagctt ggagcgagac cggagcattc
     2701 cttcgatgag ccgctgggtt tgttgagcga ttgccatcgg cgaatcgaaa aatttctcga
     2761 cgtgatgatc cgcgtgttgg atcgatcgga tcgcggaaat aagccgctcg cgcttgacga
     2821 gcgcgacgcg ctggagtcag cgctgcgtta cttcaagaac gccgccccgc ggcatacgca
     2881 ggacgaggag cagtctttat tcccacggct gcgagcgagc gacgatccgg cagcgcacgc
     2941 agcgctggcg cggatcgaag cgctcgaaga ggaccaccgg cgcgccgatg cgatgcacga
     3001 tgaggtccat cagctgtgcc ggcgatggct cgacagcttg gccatttcgg cgatggaagc
     3061 cgagcgcctc aatcagctgt tgcacgacct gcgcgacacc tatgccgggc atatcgcgct
     3121 ggaggacagc gagctgtttc cgttggccgc gcgcgtgttg aaggatgcgg atctgaaggc
     3181 ggtcggcgcg gagatggcgc agcggcgcgg ccttgacgca aacggaccga tgagccgacc
     3241 gaagtgccct tcgaagaacc ggtcataacg cggagcggaa gcgcgccgaa cttagagtta
     3301 ctccatggcc accatcccca ccgacgaact gaaaatcatc aacgagttcg gcccgcacct
     3361 ggcgcggatg tccggcgagc gcctgtccac ctcaatcgag ccggaccggc tggtgaagac
     3421 gcactgctgc ttctgcggcc agcaatgcgg catccagctg aaagttcgcg ggaacgaggt
     3481 gattggattc gagccgtggg aggattttcc gttcaaccgc ggcatgctct gtcccaaggg
     3541 tgtgaagcga tatcttcagg gttcgcatcc cgaccgcctg ctgacggcaa tacgccgcga
     3601 ccccagcgcg cccgggggat tcactcccat gccatacacc gacgccatcg ccaaggtggc
     3661 gtcggaaatt cagcggcttc agagcacata cggcaacggc gccatcgccg tcctcggtgg
     3721 cgcgagcttg acgaccgaga agacttatct ccttggcaag ttcgcccgcg tttgtttgaa
     3781 gacgccgttc atcgactaca acggccggct gtgcatggtc agcgccggcg cggcgaacaa
     3841 gaaggcgttc ggcatcgatc gcacgaccaa tccctggtcg gacatgatcg gcaccggcgt
     3901 gatctgggtc gccgggtcga acgtcgccga gtgttcgccg atcacgacga attacatctg
     3961 gcaggcgcgc gagatgggcg cgaaagtgat cgtgcaggac ccgcgcatta cgccgatcgc
     4021 gcggacctgc gatctatttc tcccggtgaa acccgggcgc gacgccgccg tcttcgcggg
     4081 tgtgctcaat ctgatgatcg agcacggctg gatcgaccgc gacttcatcg aacaacacac
     4141 cgtcggtttc gagcaggtcg ccgagtattg caagcaatgg acgcccgcgc gcaccgcgga
     4201 agtcaccggt gtcccggagc gatcgctgcg acaggcggcg gaactgtggg gtacggccaa
     4261 gagcagcttc ctcctgcacg cgcgcggcat cgaacatcat tccaatggcg tgcagaacgc
     4321 gcttggcaca atcaatctgg tcctcgcctc cggtcgaatc ggaaaatcca acagtggcta
     4381 cggcacgatc gtcggacagg ccaacggcca aggcggccgc gagcatggac agaagtgcga
     4441 ccaactgccc ggctggcgcg acatcagcaa ccccgagcat cgcaaataca tcgccggcgt
     4501 gtggggcgtc gacgagtcga gcatccccgg ccccggcgtc gatgcatacg aactgttccg
     4561 caagatcgat cgcggagaaa tcaaaggact gatctcgatc tgcttcaacc ccaagatttc
     4621 gctgccggac aacacgttca tcgcgcgggc gctgagcaag ctggagttct acgtcgcgat
     4681 tgatttcttc ctgaacgaca cagcccatca cgccgacatc gtgctgcccg gcagtttgca
     4741 ggaggaggac gaaggcacgg tcacgcaggt tgaagggcgc gtgatcaaga tcaacaaagc
     4801 ggttgattgc cccggcgaag cgcggcggga ttgggtcatc cttcaagaca tcgcccgcgc
     4861 gctgaatcga acgcacggct tt
//
LOCUS       DATIMH010000170         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1771855, whole genome shotgun sequence.
ACCESSION   DATIMH010000170 DATIMH010000000
VERSION     DATIMH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771855"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1053
                     /locus_tag="VLI90_02850"
     CDS             211..1053
                     /locus_tag="VLI90_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13169.1"
                     /translation="MFVSATARAADAPRSPATTQPLHVDLPDNASLDIATVGLSDHLG
                     EVWSADGTPIPAPASLDQSSLHWRHSAGDGQRDVGIRVNINSPAALTSSIGATPASRM
                     GMLDRPPLTFVSVDPGARTIDLRVGMPLGEAHTLATFGPFGDSADLLTDDHHFVAQPS
                     IPAVRDGELIVPIVINMPKLNEWRYALVAVLKDGERQSTDGWARRVGGVGFITYRFEK
                     THPIKPEDVVRSELQLTPFTLGDDQPTLAHARRHQRVSGEDRTTRTAAADAGASGRWA
                     VRVF"
     gene            1274..2341
                     /locus_tag="VLI90_02855"
     CDS             1274..2341
                     /locus_tag="VLI90_02855"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSV13170.1"
                     /translation="MIIASFGAALPGQEQIDKCAATWSNRAEWEARAAMLKKAILGPL
                     APWPPGTPLNPIWRAKIVHDDYTVQPVAIESMPGFYCIADLYMPAHVQGRLPAIIRAH
                     GHGKTGRYGTQVQCAAMARMGAVVMSISMVGYNDCKQYCGEHTKEHPPGPAVASLQLL
                     NTIRAVDFLTTLPEVDAHNINITGESGGGTQTFMLAAVDPRVKTSIPVVMVSATHTGC
                     RCEQGPAGLVPDMTNHAEIAAMHAPLPQLIVSDGKDWTKNVPMVEFPYIKKVYETMGA
                     PELLENVHLASEGHDYGPSKRNAAYAFFVKRGILHGAANETITPDSDEALSVFNEKTP
                     MPETALKTPAAIEAALAHLRE"
BASE COUNT          469 a          748 c          789 g          404 t
ORIGIN      
        1 ccgcggggca ttgcgaatgc tgtgctagat gctgcgaaaa ctgggaaaat ggagagaatc
       61 ggcaggggcc gatttatgtc aacgcatcgg agtatgttgg actaaataga cgcagaagct
      121 ggcccaactg cactggtgta accggaagtg ctcatggcga cacgaagatc cccggtgatc
      181 tttagcctgt tccttgcagc cgcgcttcta atgtttgtta gcgccacggc ccgggccgcc
      241 gacgcgccgc gctcgccggc caccacgcag ccgctgcacg tcgatctgcc cgacaacgcg
      301 tcgctcgaca tcgcgacggt cggcctcagc gaccacctcg gcgaagtctg gtccgccgac
      361 ggcacgccga tccccgcgcc ggcgtcgctc gatcaatcga gcctgcactg gcgacactcc
      421 gctggcgacg ggcagcgcga cgtcggcatc cgcgtgaaca tcaattcgcc ggcggcgctg
      481 accagcagca taggcgccac cccggcgtcg cgcatgggga tgctcgatcg cccgccgctc
      541 acgttcgtct ccgtcgatcc gggcgcgcgg accattgacc ttcgcgtggg tatgccgctg
      601 ggggaagcgc acacgctggc gacgttcggg ccgttcggtg attcagccga tttgctcacc
      661 gacgatcatc actttgtcgc gcagcccagc atccccgccg tgcgcgatgg cgaactgatc
      721 gttcccatcg tgatcaatat gccgaagctg aacgagtggc gatatgcact ggtcgcggtg
      781 ctgaaggacg gcgagcggca atctaccgac ggctgggcgc ggcgagtcgg cggtgtcggt
      841 ttcatcacct accgcttcga gaaaacgcac ccgatcaagc cggaggacgt cgtccgatcc
      901 gaactgcagc tgacgccgtt caccttgggt gacgatcagc caactctcgc tcacgcccgg
      961 cgtcaccagc gcgtcagcgg tgaagatcga accacccgaa ccgctgccgc cgatgcagga
     1021 gccagcggca gatgggctgt gagagtgttc tgagcaggcg aatcgagaaa ttggaggcag
     1081 gacaatcgca tcctcgatta ggccagcagc cgcaactgta gctgcgacgc ctgcgtcgcc
     1141 tccggctcca acaggcgatg caggcatcgc ggctacagga accagaatga cgattgcctt
     1201 agatttagaa attggaggac cgtcgatgac cggaacatcg caccgcgttc gccgcggttt
     1261 tatattgttg ttcgtgatca ttgcatcatt tggcgctgcg ttgccggggc aggagcagat
     1321 cgacaagtgc gctgcgactt ggtcgaaccg cgcggagtgg gaagcgcggg cggcgatgtt
     1381 gaagaaggcc atcctgggcc cactcgcgcc ctggccgccg gggacgccgc tgaatccgat
     1441 ttggcgggca aaaatcgttc acgatgatta caccgtgcag ccggtggcga tcgagtcgat
     1501 gcccggcttc tactgcattg cggatctgta tatgccggcg cacgtgcagg gccggctgcc
     1561 ggcgatcatc cgcgcgcacg gccacggcaa aaccggccga tatggcacgc aggtgcagtg
     1621 cgccgcgatg gcccgcatgg gggcggtcgt gatgtcgatc agcatggtcg gttacaacga
     1681 ctgcaagcag tattgcggcg agcacaccaa ggaacacccg cccgggccgg ccgtggcttc
     1741 gctgcaattg ctcaacacca ttcgcgcggt cgattttctt acgacgctgc cggaggtgga
     1801 cgcgcacaac atcaacatca ccggcgaatc cggtggcgga acgcagacat tcatgctggc
     1861 agccgtcgat ccgcgcgtga agacgtcgat cccggtcgtg atggtgtcgg ccacccacac
     1921 cggttgccgc tgcgagcaag gccccgcggg gctggtgccg gacatgacca atcacgcgga
     1981 gatcgcagcg atgcacgcgc cgctgccgca gctgatcgtg tcggatggga aggactggac
     2041 gaagaacgtg ccgatggtgg agttcccgta tatcaaaaag gtatacgaga cgatgggcgc
     2101 gcccgagctg ctggagaacg tacacctggc gagtgaaggc cacgactacg gcccatcgaa
     2161 gcgcaacgcg gcgtacgcgt tcttcgtgaa gcgcggcatc ctgcacggcg cggcgaacga
     2221 gacgatcacg ccggacagcg atgaggcgct gagcgtgttc aacgagaaga cgccgatgcc
     2281 cgagacggcg ttgaagacgc cggcggcgat cgaggcggca ttggcgcacc tgcgcgagta
     2341 aacatactgt agccgcgacg cttgcgtcgc ctctttgggc cggaggcgac gcaggcgtcg
     2401 cagctacaac
//
LOCUS       DATIMH010000171         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4948484, whole genome shotgun sequence.
ACCESSION   DATIMH010000171 DATIMH010000000
VERSION     DATIMH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4948484"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VLI90_02860"
     CDS             <1..395
                     /locus_tag="VLI90_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV13171.1"
                     /translation="RNNLAYTVLRRGGDLKEAKALAERAVADAPSNSAYQDTLARIYA
                     RLGDLPAALTTFQNAVRDDAGNVEALIGLADTQTRTGNRDEAASTLERVETILHSNPM
                     LPDAVRQQLDEVRTSLKKPAETTSADVK"
     gene            complement(449..832)
                     /locus_tag="VLI90_02865"
     CDS             complement(449..832)
                     /locus_tag="VLI90_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV13172.1"
                     /translation="MPTILVVDDTALAREAVAKLLEYEGFTVLRAEHGRDAWAMMYDH
                     TPDLVLLDLMMPEMDGVTLLRMIRRNDRWQELPVIVLTGVTDDQFLIQRARELKIQDL
                     VPKATFGFDDLLTRVKRHLPKAMAS"
     gene            1113..1676
                     /locus_tag="VLI90_02870"
                     /pseudo
     CDS             1113..1676
                     /locus_tag="VLI90_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
BASE COUNT          335 a          507 c          536 g          298 t
ORIGIN      
        1 cccgcaacaa tctcgcgtat acagtgctgc gccgcggcgg cgatctgaag gaagcaaagg
       61 cgctggccga acgcgcggtg gcggatgcgc ccagcaacag cgcatatcag gatacgctag
      121 cgcggatcta tgcgcgtctg ggcgacttgc ctgcggctct gaccacgttt caaaatgccg
      181 ttcgcgatga cgcggggaat gtggaagcgt tgatcggatt agcggacacg cagacgcgca
      241 cggggaatcg agatgaagcc gccagcacgt tggagcgggt ggagacgatt ttgcacagta
      301 acccgatgct gccggatgcg gtgcggcagc agttggatga ggtgcggacg tcgctgaaaa
      361 agccggcgga gactacttct gcggatgtga agtaatttgc gcagacacac gtgcccgtgc
      421 ggggctaccg ctacgctttc gaaaagttct acgacgccat cgctttgggg agatgccgct
      481 tcacgcgggt caacaggtcg tcgaatccga acgttgcctt cggcaccagg tcctgaattt
      541 tcaattcgcg cgcgcgttgg atgaggaact ggtcgtcggt gacgccggtc agcacgatca
      601 ccggcagctc ctgccagcgg tcgtttcggc ggatcatccg cagcagcgtg acgccgtcca
      661 tctcaggcat catcaggtcg agcagcacca ggtccggcgt gtgatcgtac atcatggccc
      721 acgcgtcgcg cccatgctcg gcgcgcagga cggtgaagcc ttcatactcg agcaattttg
      781 ctaccgcttc acgagcgagc gccgtgtcat caacaaccag aattgtcggc atgcagatgc
      841 ctccctgctt tgacactttg ccgtgcgaag gtcgattggt caatggcaaa gttcgctttc
      901 gtttgatcgc aagcggcgcg cccgcgcgcg ctggcgcgcg tgtcgcgatt ggccgcgcag
      961 gcgcgacacg cgccgctctt tggtagagtc tctcgatcat gagcgctccc acctcaccgg
     1021 caccgacgac agacctcctc tcgcccaagg ccgaacaaca ttccagattc tggcaaaacg
     1081 cgcatcagta tttcgatcgc gtgtaccaga cgatgaacct cgatcaatcg tggcgcgcgg
     1141 tactgtcgtc gcccaagcgc gtgttgaccg tctcgtgtcc ggtgaagatg gacaacgggc
     1201 agatccaggt cttcaccggt tatcgcgcgc agcacaacaa cgcgcgcggg ccgttcaagg
     1261 gcggcatccg ttatgacagc ggtgtcaatc gcgacgaagt gatggcgctg gcgatgctgc
     1321 agacgtggaa gaacgcgctg gcggatcttc ccttcggcgg cgccaagggc ggcgtggtgt
     1381 gcgagcccaa gacactgtca gccggtgaaa aggaacgcct cacccgacga tagaccagcg
     1441 aaatcatgcc gatcatcggc ccgcagcacg acatccccgc gccggacgcc ggcaccgatg
     1501 ggcaggtcat gagctgggtg ctcgacacgt acagcatgat ggccggctac caggcgctgg
     1561 cggttgtcac tggcaaaccg atcgcgctgg gcggcagcgt gggtcgcgaa gaagccacgg
     1621 gccgcggcgt catgaacgtg ctgcgaaagt tcctcgccac gcagcacaag acgatc
//
LOCUS       DATIMH010000172         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3792979, whole genome shotgun sequence.
ACCESSION   DATIMH010000172 DATIMH010000000
VERSION     DATIMH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3792979"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1349)
                     /gene="tyrS"
                     /locus_tag="VLI90_02875"
     CDS             complement(81..1349)
                     /gene="tyrS"
                     /locus_tag="VLI90_02875"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSV13173.1"
                     /translation="MMDIYSELEWRGMLSESTEGVREALLAAPLTGYIGFDPTAPSLH
                     VGSLLTVMGLARLQRFGHNPIAIVGGGTGMIGDPSGKSQERVLLSSADVEANVAGIRQ
                     QLEKFLDFNAPRNAARIVNNADWLGTIDVLSFLRDVGKHFTVNYMLQKESVARRLESE
                     EGISYTEFSYLVLQAYDYLQLFDRYQCTLQLGGSDQWGNITAGIELIRKLRAQKTHGL
                     VWPLMTTASGVKFGKTEAGAVWLDPVRTTPFHFYQFWLNTDDRDVIRYLKCFTFLERD
                     SIEALARATQEKPEAREAQRALAREVTTLVHGAEHVARAERAASVLFTDNLTAADVDD
                     VLMVLADAPSTEMALPDRGISLVELLAIVKLAASKSEALRLIKSGGVYVNNVRAADEK
                     ALVSAADAIGGQVIVLRKGRREQHVVRVRS"
     gene            1508..2323
                     /locus_tag="VLI90_02880"
     CDS             1508..2323
                     /locus_tag="VLI90_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSV13174.1"
                     /translation="MSRRDLLKGFAAAGIGVSAGAVAHGYFYERFRIGLTRETVNMPG
                     LPPALSGLRIGLITDIHRSDTVTHELIDRAVRILLAESPDLIVLGGDYVTWGDRRFVG
                     AAADALAPLTAPQGVFAVLGNHDNDHDMPPPLAAKGFVVLRDARTRLIIRGETLDLAG
                     IRYWTRKPSEIAYVLRGGSPNLILLAHTPMRLIEAASLGVPLVLSGHTHGGQIVLPGI
                     GAVAAREFPVIAGGGRRENTRIFVSRGVGTVYVPVRLNCPPEVAVLTLRPVEV"
BASE COUNT          458 a          815 c          789 g          501 t
ORIGIN      
        1 ccgcggcaat gttcaatgct caatgctcaa tgtccaatgc tcccccgtgc gtcggctctg
       61 ccattcaaca ttgaacattg tcacgatcgc actcgcacca cgtgctgttc ccgtctgccc
      121 ttcctgagca cgatcacctg accaccaatc gcatccgctg ccgacaccag tgccttttca
      181 tccgccgcgc gtacgttgtt cacgtagacg ccgccgctct tgatgagccg cagggcttca
      241 cttttggaag cggccagctt gactatggcg agcagctcga cgagtgaaat accgcgatcc
      301 ggcagggcca tttcggttga aggcgcgtct gccaggacca tcaacacgtc gtcgacatcc
      361 gccgcagtga ggttatcggt aaagagcacc gaggccgccc gctccgcgcg ggcaacatgc
      421 tcagccccgt gaaccagcgt tgtaacctca cgcgccagcg cgcgctgcgc ctcgcgcgct
      481 tccggcttct cctgggttgc gcgcgcaagt gcctcgatgc tgtcgcgctc caggaacgta
      541 aaacacttca gatagcgaat cacatcccga tcgtcggtgt tgagccagaa ctgataaaag
      601 tggaacggcg tggtgcgaac cggatcgagc cacaccgcgc cggcctccgt ctttccaaac
      661 ttcacgcccg acgccgtcgt catcagcggc cacaccagac cgtgcgtttt ctgggcgcgc
      721 aactttcgga tcagctcgat gccagccgtg atgttgcccc actggtcgct gccgcccaat
      781 tgcagcgtgc actggtagcg atcgaagagc tgcaggtagt cgtacgcctg caacaccagg
      841 tagctgaact cggtgtagga gatgccctct tcgctttcga gtcgacgcgc gactgactct
      901 ttctgcagca tgtaattgac ggtgaagtgc ttgcccacgt cccgcaagaa cgagaggacg
      961 tcaatcgttc cgagccagtc agcgttgttg acgatgcgcg cggcatttct cggcgcgttg
     1021 aagtcgagaa acttctcgag ctgctgccga atccctgcga cgttggcttc aacgtcagcg
     1081 ctcgacagca gcacgcgttc ctgagacttg ccgcttggat cgccgatcat tcccgtgccg
     1141 ccgccgacga tggcaatcgg gttgtgacca aatcgctgca gtcgcgcaag gcccatgacg
     1201 gtcaacagcg agccgacgtg aaggctgggg gcggtcgggt cgaagccgat gtagccagtc
     1261 agcggcgcag ccagcagcgc ctcgcgaacg ccctccgtac tctccgagag catgccgcgc
     1321 cattcgagtt ccgagtagat gtccatcatg tcgtcgcgtc ggggtcgtac tatatcgcga
     1381 gaagaatggg tcgcgaatgc cgcgcgaccg tgggttgtgg tggccgacaa tttggttcgc
     1441 ccgccggcaa tcggcgtcgg ccgcgctaac ataacgccca atgcccccga ccggatccgc
     1501 tcgcagcgtg agcaggcgcg acctcctcaa agggttcgct gcggccggca ttggggtgag
     1561 cgccggcgcg gtggcgcacg ggtacttcta cgaacggttc cgcatcggcc tgacgcgcga
     1621 gacggtcaac atgccggggc tgccaccggc gttatccggg ctgcgcattg gactcatcac
     1681 cgacatccac cgcagcgaca cggtgacgca tgaattgatc gatcgcgccg tgcgaatact
     1741 gctggccgag tctcccgact tgatcgttct gggtggggac tacgtgacgt ggggagatcg
     1801 gcggttcgtc ggagcggcgg ccgacgcgct cgcgccgctc accgcaccgc aaggtgtgtt
     1861 tgccgtgctc ggtaatcacg acaacgatca cgatatgccg ccgccgctcg ccgccaaggg
     1921 tttcgtggtt cttcgtgatg cgcgcacccg gctgatcatc cgcggtgaaa cgctcgacct
     1981 cgccggcatc cgctactgga cccgaaagcc ctccgaaatt gcctacgtgc tgcgcggcgg
     2041 ctcccccaac ttgatcctgt tggcgcacac accgatgcgg ttgattgaag cggcctcgct
     2101 cggagttccg ctggtactca gcggccacac gcatggcggt cagattgtgc tgccggggat
     2161 cggcgccgtc gcagcgcggg agttcccggt aatcgccgga ggcgggcggc gcgaaaacac
     2221 ccgcatcttc gtcagccgcg gcgtgggtac cgtatacgtg ccagtccgcc tgaattgccc
     2281 gccggaagtc gcggtcctga cacttcggcc tgtcgaagtc tgaagccggt tgcaatccgc
     2341 cccgctgagg cgcataatca cgccgtcgca aattttgaat aatcaaaatt acagtatcga
     2401 gcagtgaaga cttggaggga acatggcgag gcgtttagga cgatgggcgt tcgcgacttc
     2461 catagtggca ggcctggtgc tggtgctcgc tgggaccttt cgtgatgcgt ggctgtgggc
     2521 ttatgtcggc gtctgggcag cggcgagtgg gtatgcgtta ctg
//
LOCUS       DATIMH010000173         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3505068, whole genome shotgun sequence.
ACCESSION   DATIMH010000173 DATIMH010000000
VERSION     DATIMH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3505068"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VLI90_02885"
     CDS             <1..311
                     /locus_tag="VLI90_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSV13175.1"
                     /translation="AHHRPNQLSGGQRQRAAIARALINEPALILADEPTGNLDTAMAA
                     TIMELFASLNRTGTTVIVVTHDMEVARHTHRMIRMRDGRIVPTQHEEREPQMHTDEHG
                     "
     gene            378..1619
                     /locus_tag="VLI90_02890"
     CDS             378..1619
                     /locus_tag="VLI90_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV13176.1"
                     /translation="MFFLLGIWNGIGEIRAHKLRSMLTITCVLLGVASLVLIAAFITG
                     LFRRWEVYSQEMGWAQKVQVDDSVPPDEQQHIAAISPGRTLADARAIERLSHYAAAVS
                     PMTDLRGNMSFNGKVYSERVHGVTPGVLSVDRYEVARGRFITDLDLARGEPVIVLGSE
                     PVREIFKGIDPMGQVVQVNGQPFRVVGLLKHYEEMDGQYNILERKNRTAFVPITSAQL
                     RISGNDRLDQLNVRVNDLSNLGRLVDEVTAILTRTHRGINDFEAYTEQENIAELNAMR
                     RNFFVVGCGIGAITLLVGGIGIMNLMLASINERVREIGIRKAIGAWNRDIFVQFMAES
                     ITLSAFGGVLGVGVGIVVVRMLRHAMGGGSGGMGSSPPVLSVPAVIIGLGFSVFVGVV
                     AGLYPAFRASRLDPIEALRYE"
     gene            1738..>3154
                     /locus_tag="VLI90_02895"
     CDS             1738..>3154
                     /locus_tag="VLI90_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSV13177.1"
                     /translation="MVVFLVAGCSFPHEQDVRLVVSERQRLQALGDPATLTATTRPAP
                     ADAGRWAGPLTTTQPATRQASIGSVLSLTIEQAVLMALDRNRDLVVQRFNPQISQLAE
                     EGLYGQFDPTVAASASVSRTWPTTGQSGSKSIQAQAAIQQFFATGTTVSVGLSAIDSG
                     THLYGDQRDATTSVRAGISVTQSLLQGAGLRVNLASIRQAELDVLQSQYQLRGFTEQL
                     ASDTEQTYIDYVLAERELEIAESALRVSQQQLDETDAMIRSGKSATSDRAAALATVAQ
                     RKEDLINARSALEQVRLRFLRFVNADTASLAGNWGARLSLIQPAMPTGALESVDAHVR
                     VALLYRSDLNQARLQAERGDLDVIKTRNGLLPRLDLFATLGRTWYFHSPAPAGFTNGG
                     AYDASVGVNFDYPLLNRSARAAYLSSRASRDQAIEAVANLAQLVELDVRLAYEETVRA
                     QEQIAATTATLAARDEALRVEQ"
BASE COUNT          533 a          904 c         1138 g          579 t
ORIGIN      
        1 gcgcgcatca tcggccgaat cagttgtcgg gcgggcagcg ccagcgggcc gcgattgccc
       61 gcgcgctgat caacgagccg gccctcatcc tggcggatga accgaccggc aatctcgaca
      121 ccgcgatggc ggcgaccatc atggaactgt tcgcgtcgct gaatcgaact gggacgaccg
      181 tgattgtcgt cacgcacgat atggaagtgg cgaggcacac gcatcggatg atccgcatgc
      241 gcgacggacg catcgtgccg acgcagcatg aagagaggga accacagatg cacacagacg
      301 aacacggata aggcgaaaac agtgctgttc catctgtgtt catctgtgtg catctgtggt
      361 taatcgagct tgtccttatg ttttttctcc tcggcatctg gaacggcatc ggcgagatcc
      421 gcgctcacaa gctgcggtcg atgctgacga tcacttgcgt gctgctgggc gtggcgtcgc
      481 tggtgctgat cgcggcgttc atcaccggat tgttccggcg atgggaggtt tattcgcagg
      541 agatgggctg ggcgcagaag gtgcaggtgg atgactcggt gccgcccgac gagcagcagc
      601 acattgccgc gatatcgccc gggcgaacgc tggcagatgc gcgggcgatc gagcggctgt
      661 cgcattacgc cgcggccgtt tcgccgatga ccgatcttcg cgggaatatg tcgttcaacg
      721 gcaaggtgta ttccgagcgc gtccacggcg tgacgccggg ggtgctgagt gtcgatcgtt
      781 atgaagtcgc gcgcgggcgg ttcatcacgg acctcgatct tgcgcgtggc gaaccggtga
      841 tcgtgctggg cagcgagccg gtgcgcgaga tttttaaggg catcgatccg atgggccagg
      901 tcgtgcaggt gaacggccag ccgttccgcg tggttgggtt gctgaagcat tacgaagaga
      961 tggacgggca gtacaacatc ctcgagcgaa aaaaccgcac cgcgttcgtg ccgatcacct
     1021 cggcacagct gcggatcagc gggaacgatc gattggatca gctgaacgtt cgcgttaacg
     1081 acctgtcgaa cctgggccgg ctggtggacg aggtgaccgc gatcctcacg cgcacgcacc
     1141 gcgggatcaa cgatttcgaa gcctacaccg agcaggaaaa tatcgctgaa ctgaacgcga
     1201 tgcggcgaaa tttttttgtc gtcggttgcg ggatcggcgc gatcacgctg ctggtcggcg
     1261 ggatcggaat catgaacctg atgctggcgt cgatcaacga acgggtgcgc gagatcggca
     1321 tccgcaaagc catcggcgcg tggaaccgcg acatcttcgt gcagttcatg gcggagtcga
     1381 tcacgctcag cgccttcggt ggagtattgg gcgtgggcgt gggcatcgtg gtcgtgcgga
     1441 tgctccgcca cgcgatgggc ggcggcagcg gggggatggg atcgtcccca ccggtgctgt
     1501 cggtgccggc ggtgatcatc gggctggggt tcagcgtctt cgtcggcgtc gtcgccgggt
     1561 tgtacccggc gtttcgcgcg tctcgtttgg atccgatcga ggcgctgcgc tatgagtgag
     1621 cgcaacgcga gatccatccc aatgcgtagc ggtagccccg caggggcacg tgtttcttcc
     1681 tccagcgcga aaaacgcgtg cccctgcggg gctaccgcta cgcggggagt tgtgtggttg
     1741 gttgtttttc ttgttgccgg ttgctcgttc ccgcacgagc aggatgtgcg actggttgtc
     1801 tcggagcggc agcggctgca ggcgctgggt gatccggcga cgctgacagc cacaacgcgg
     1861 ccggcgccgg cggacgcggg gcgttgggcg gggccactca ctacgacgca accggcgacg
     1921 cgccaggctt cgatcgggtc ggtgctgtcg ctgacgatcg agcaggcggt gttgatggcg
     1981 ctggatcgca accgcgatct ggtggtgcag cggttcaatc cgcagatttc acaactggcg
     2041 gaagaggggt tgtacgggca gttcgacccg acggtggccg cgtcggcgtc ggtgtcgcgc
     2101 acctggccga ctacggggca gtcgggcagc aagtcgatcc aggcgcaggc cgccatccaa
     2161 cagttcttcg cgaccggcac gaccgtatcg gttgggctat cggcgatcga cagcggcacg
     2221 cacctgtacg gcgatcagcg cgacgcgacg acttccgttc gcgccggcat cagcgtcacg
     2281 cagtcgctgc tgcagggcgc cggccttcgc gtcaacctgg cgagcattcg gcaggcggag
     2341 ttggatgtac tgcaatcgca atatcagttg cgcggcttta ccgagcagct ggcgtcggac
     2401 acggagcaga cgtacatcga ttacgtgctg gccgagcgcg agctggagat cgcggagtcg
     2461 gcgctgcggg tctcgcagca gcagctggac gagacggacg cgatgatccg ctccggcaaa
     2521 tcagcgacat ctgatcgggc ggcggcgctg gcgacggtgg cgcagcggaa ggaagatctg
     2581 atcaacgcgc ggtcagcgct ggaacaggtg cggctgcgct tcctgcggtt cgtgaacgcc
     2641 gacaccgcct cgttggcggg taactggggc gcgaggctgt cgctgatcca accggcgatg
     2701 ccgacagggg cgctggagag cgtcgatgcc cacgtgcggg ttgcgctgct gtatcggtcc
     2761 gacttgaatc aggcccgcct gcaggcggag cgcggggatc tggatgtcat caagacgcgc
     2821 aacggattgc tcccgcggtt ggacctgttc gcgacgctgg gtcgcacgtg gtacttccac
     2881 tcgccggcgc cggccggctt cactaacggc ggggcgtacg acgccagcgt cggcgtgaat
     2941 ttcgattatc cgctgttgaa tcgttccgcg cgggcagcgt atctgtcgtc gcgcgcgtcg
     3001 cgcgatcagg cgatcgaggc ggtggcgaac ctggcgcagt tggtcgagct ggatgttcgg
     3061 ctggcgtacg aggagacggt gcgggcgcag gagcagatcg cggcgacgac ggcgacgctg
     3121 gcggcgcgcg acgaggcgct gcgggtggag cagg
//
LOCUS       DATIMH010000174         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1483869, whole genome shotgun sequence.
ACCESSION   DATIMH010000174 DATIMH010000000
VERSION     DATIMH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483869"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VLI90_02900"
     CDS             <1..824
                     /locus_tag="VLI90_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13178.1"
                     /translation="SVSSGKLANVMGTSSRRNWDFFTSDNSTPDNSTAHNSVLQLGVG
                     DTLKWSMSFTLPNGATSNAGNASRDFRIGIYTDPTDARVQTDTNSDSGGATNPWGDAL
                     GYGMKIPLNGNLSNTTSLFQIGKRTSSASTTLFGSDSSYTYAPVGGAAQGLSANTSYT
                     AEMVLNEVSATDMKITANFYQGTTLLSTQTVDDLGSSVGGTANIAGSFAAASSIYTKF
                     DQLAFRMSSNTTTSEIDFTNWKVDYTAAVPEPTMIGVICLAGYAMLARRPRRERA"
     gene            1176..1598
                     /locus_tag="VLI90_02905"
     CDS             1176..1598
                     /locus_tag="VLI90_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSV13179.1"
                     /translation="MRVMVLIKANRDTEAGVMPSEELLTAMGRFNEELVKAGVMLAGE
                     GLKPTSKGARIQFSGSKRAVVDGPFTETKELVCGYWLWKVNSMAEAVEWAKRCPNPTG
                     DQAEMELRPLFEMEDFGAEFTPELRKQEARLRAQAAAH"
     gene            1688..2110
                     /locus_tag="VLI90_02910"
     CDS             1688..2110
                     /locus_tag="VLI90_02910"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSV13180.1"
                     /translation="MHIQPYLNFDGRCEEAIEFYKRAIGATVLFMMRMKDSPEKPPKG
                     MEVPGSDNKIMHASLRIGDSVMNASDGRCTGSGKFDGISLSLNAGNDAEAARLFKALG
                     DGGQIQMPMSKTFFASSFGMVTDRFGVMWMVIAGQQAV"
     gene            2267..>2540
                     /locus_tag="VLI90_02915"
     CDS             2267..>2540
                     /locus_tag="VLI90_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSV13181.1"
                     /translation="MRYLCLGYHDEQTWAAMSDADRRALLADNATYEQRLRGGGHYID
                     GKALQDRRSTATLRFDDGGRVSITDGPFAETKEQLGGVMVLEAADLN"
BASE COUNT          535 a          742 c          837 g          426 t
ORIGIN      
        1 tgagcgtctc gtccggtaag ctggccaacg tcatgggcac tagctcgcgg cggaactggg
       61 atttcttcac cagcgacaac agcacgccgg acaacagcac cgcccacaat tcggttttgc
      121 aactgggcgt gggtgacacg ctgaagtggt cgatgagctt cacgctgccc aacggcgcga
      181 cgagcaacgc cggcaacgcc agccgggatt tccgcatcgg catctacacc gatcccaccg
      241 acgcgcgcgt gcagaccgac accaacagcg atagcggcgg cgcgaccaac ccctggggcg
      301 acgcccttgg gtacggcatg aaaatcccgc tcaacggaaa tctgtccaac acgacctcgc
      361 tgttccagat cggcaagcgc accagcagcg ccagcacgac gttgttcggc agcgatagct
      421 cgtacaccta tgcccccgtg ggtggcgcag cgcagggcct gtctgccaac accagctaca
      481 cggccgagat ggtgctgaac gaagtcagcg cgaccgacat gaagatcacc gcgaacttct
      541 accagggaac gacgctgctc tcgacgcaga cggtggacga cctgggctcg agcgtgggcg
      601 ggaccgccaa catcgccggc agcttcgccg cggcgtcgag catttacacg aagttcgacc
      661 agctggcgtt tcgcatgagc agcaacacga cgacgtcgga gatcgacttc accaactgga
      721 aggtcgatta caccgcggcg gtgccggaac cgacgatgat cggcgtcatc tgcctggccg
      781 gttacgcgat gctcgcccgc cgcccgcggc gggaacgcgc gtaggcgcgc gctttgcccc
      841 gataggtctg ggagagaatg ccgaggcccc ggcgagcgca agctcaccga ggcccggttc
      901 tttttttggt tcatcttcgc agctggcgcc gggatggaat gcaaccgcga aggcacgaac
      961 gcggccgagg tcgcgttgta gcacgccggg tagggtgagg aagaagtcgg ttcttgtgat
     1021 acgcacggcg cggcggttgc tttttgcacc atggcacggg cgagacgccc gtgctatgaa
     1081 ctaaaaaatt cgtcgctgtc gattcgccag ggcttcgcac gattactcag tgttcgagat
     1141 aaccacagac aactaacccc gaaaggagca ccgcaatgcg agtcatggtt ttgatcaagg
     1201 cgaatcgaga caccgaggcc ggcgtgatgc ccagcgaaga gctgctgacg gcgatgggcc
     1261 gctttaatga ggaactggtc aaggccggcg tgatgctggc cggcgagggg cttaagccga
     1321 cgtccaaggg ggcgcggatt caattctccg gcagcaagcg agccgtcgtt gacggcccgt
     1381 tcaccgagac caaggagctg gtctgcggct attggctgtg gaaagtcaat tcgatggcgg
     1441 aggcggtcga atgggcgaag cgctgcccga atccgaccgg ggaccaggcg gagatggagc
     1501 tgcgcccgct gtttgagatg gaagatttcg gtgcggagtt cacgccggaa ctgcgcaagc
     1561 aggaggcccg gctgcgggcg caagccgcgg cgcattaggc tattccgaca cggggcgaaa
     1621 aaaatttttt taccccgtgt cgattccgaa cacactcgtt cgactaaccg ggtaaaggag
     1681 atcgatcatg cacattcaac cgtatctgaa cttcgacggc cgatgcgagg aagcaatcga
     1741 gttttacaag cgcgccatcg gcgcgacggt gctcttcatg atgcgcatga aggacagccc
     1801 ggagaagccg cccaaaggga tggaggtgcc cggcagcgac aataagatca tgcacgcgtc
     1861 gctgcggatc ggcgattcgg tcatgaatgc atcggacggc cgatgcaccg gcagcgggaa
     1921 gtttgatggg atctcgctgt cgctgaatgc cggcaacgac gcggaggcgg cgcggctgtt
     1981 taaagcgctg ggagacggtg gtcaaataca gatgccaatg agcaagacgt tcttcgcgtc
     2041 gagcttcggc atggtcaccg atcgcttcgg cgtgatgtgg atggtgatcg caggacaaca
     2101 agcggtctaa gcgcgacggc gatcgcgcgc aggtgcgcca ctaatagccg caggcgaaag
     2161 ccagcgggtg cgttggaact gcaaccggcc tggcatcgaa acacgcgcgc tgtcgcctgc
     2221 ggctattaaa atccttctcg cgcccctgat cgatccggag ttcatcatgc gttatctttg
     2281 cctcggctat cacgacgaac agacgtgggc cgcgatgagc gacgcagacc ggcgcgcgct
     2341 gctggccgac aacgcgacgt atgaacagcg gctgcgcggc ggtgggcatt atattgatgg
     2401 caaagcgctg caggaccggc gatccaccgc gacgttgcgg ttcgacgacg gtgggcgcgt
     2461 gtcgatcacc gatggtccgt tcgccgagac caaggaacaa ttgggcggcg tgatggtgct
     2521 ggaggcggcg gacctgaacg
//
LOCUS       DATIMH010000175         5348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_329452, whole genome shotgun sequence.
ACCESSION   DATIMH010000175 DATIMH010000000
VERSION     DATIMH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329452"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..386
                     /locus_tag="VLI90_02920"
     CDS             165..386
                     /locus_tag="VLI90_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13182.1"
                     /translation="MIVLAPPYAVCACGVIGSSLVERQSDSSRFLTARNYFSGFLNAT
                     AEGAEHAEADAEKTSVKSLLTADYADLAD"
     gene            532..960
                     /locus_tag="VLI90_02925"
     CDS             532..960
                     /locus_tag="VLI90_02925"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13183.1"
                     /translation="MEVVVDLRNGELVAQDHVETTDDASALVLKADRSAIHADGVDLS
                     MITVSAIDNDGRAVPTADEEVTFTITGQGKIIGVGNGDPSSHESDKANHRRLFNGLAQ
                     VIVQSGRSAGKIELTASAPQMNPATIEIDAQPSPPLPQVP"
     gene            complement(1206..2873)
                     /locus_tag="VLI90_02930"
     CDS             complement(1206..2873)
                     /locus_tag="VLI90_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HSV13184.1"
                     /translation="MGKGSGHRAGNRNRRRHRNSLRASAVHPKSPSIFEPLERRQLLS
                     VSVSGLVLVNADTGQDIMPLSGRVPLDLSTLPTRNLNVRADTGVGTSSVRFGYDGVSR
                     YHIENVAPFDLAGDNGKGGYYAWTPDTGTHKLTATPYSAAKATGTRGSRVSVQLVVTD
                     GSPTVTPPNPDPQPQPNPQPQPDPQPQPQPNPQPQPQPQPQPQPTGVTPPPVGGNWSL
                     KFADEFNATPATPTWVDTLWGTTHFSGEIENYTPNNVNVSGGNLNLTARKESSNGFPY
                     TSGLIDTGGDFATVGKNLPGFSIKYGYLEARMRLNKGSGLWPAFWMMPTPDANGNYHD
                     GDGEIDIMEQVGTDPTRTEAHLHHNGTQGKSFDTGLDLSAGFHTYGLDWQPDHMTWYF
                     DGKPYYTITSNVPNVAEYIILNLAVGDANSWPGAPDSGTPIPATMQIDYVRAFQLPGQ
                     TATGPAAVSIAMSALAAPAASIATTPLDATQLVTGSLPDFSNTPDDASQSLGLQTDAH
                     IDYSKVTDDDIDRHHRGSDDDGGDDDDGDSSPWSDVAWTNDDLTDLS"
     gene            3567..3986
                     /locus_tag="VLI90_02935"
     CDS             3567..3986
                     /locus_tag="VLI90_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV13185.1"
                     /translation="MDRAGKRILLVEDHADTLATFDRLLGRWGYAVCTASCAASASAA
                     IERERFDLLICDIGLPDQPGTSVMRAMRAANPTVPGIALTGYGMEGEIETIMAAGFTR
                     HLLKPVEMQILQTAIEDVLKQRTPAITQVTGGCRPAI"
     gene            4021..5265
                     /locus_tag="VLI90_02940"
     CDS             4021..5265
                     /locus_tag="VLI90_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HSV13186.1"
                     /translation="MPTSRSAAPIVYCFIAIFVSLFMPGSGQAQEKVKVEIRVTVPKE
                     TPADSTIYIAGDQPAVGQWKADGAALKPAGDHVYSIQLELPKGGTMEYKIDRGSWETV
                     EKDAKGDEVGNRTLSLDGDKTENITVATWAGATAAGGADKPAVPAAPAAPKASGVVGD
                     VRYHQSFHSDILHNDRRIIVWLPPTYSTDTSARFPVLYLHDGQNCFDASTSYAGEWRA
                     DETADALIRANKMKPIIMVAVANAGPDRMNEYTPTRDAVRNAGGRGEQYAQFLITEVK
                     PFIDRTYRTQADRENTAAAGSSLGGLISLYLAYKHGDIFGAAGVISPSLSWDNASLMK
                     QIEADPSPLKAERIWLDMGGDEGNPTEAAQGVANAKSLAATLQGTIGEKNLKYLEVPG
                     AKHNEAAWAARFDQVLVFLFGT"
BASE COUNT          967 a         1656 c         1741 g          984 t
ORIGIN      
        1 ggtcggtcgc ttcggcatga atcgtgacgc cgaccgccgt ggtgctgatc ggcacgatcg
       61 tccccaccgg gggcgtcggg gtcaatatag cgctgccgct cagcgacgcg gacaacaacc
      121 ggcgatcttc aagagcttcg atgacgattg gattggatga gttcatgatt gtgcttgcgc
      181 ctccgtatgc cgtctgtgcg tgcggtgtga tcgggagcag cttagtggaa cggcagtcgg
      241 actcaagcag atttctgaca gcgcgcaact atttttcagg gtttttgaat gcaaccgccg
      301 agggcgccga gcacgccgag gcggacgccg agaagacatc agtgaagagt ttattaaccg
      361 cagattacgc agatttggca gattgaagat agctttttct gaatctgcga aatctgccta
      421 atctgcggtt aaaactcttc tcttttcggc gtttatctcg gcgctctcgg cggttgcctt
      481 gccgcgcggt acagcgattt ctctcggagg atggtgaatc atgcggaatc catggaagtc
      541 gtcgttgatc tgcgcaacgg cgagcttgtc gcgcaggatc atgttgagac gaccgacgat
      601 gcatcagcgc tggtgctgaa ggcggatcgt tcggcgatcc acgccgatgg cgtggacctg
      661 tcgatgatca cggtcagcgc gatcgacaac gacggccgag cggtgccgac cgcggatgag
      721 gaggtcacgt tcaccatcac cggccagggg aaaataatcg gcgtcggcaa cggcgatccg
      781 agcagccacg aatccgataa ggcgaatcac cgccggctgt tcaatggact ggcgcaggtg
      841 attgtgcaaa gtggacgatc ggccggaaaa atcgagctga ccgcgagtgc gccgcaaatg
      901 aacccggcga cgatcgagat cgacgcgcag ccgtcacccc cgctgccgca ggtgccttag
      961 atagcgcgca cgcctccgga tgatgaagga gccccgcatg tacatgcggg gcttcatccg
     1021 cacgacgcac gcattgatgt cgcagcatcg acttcctcgt cgcgcgagtc atcgttcata
     1081 gacttccggc aagatccttc gactccggcg gcgtaccgcc tgcgctcagc atgacgcgcg
     1141 tgcgagggcg cgcgcgtggc cgtgctcgag tgctcgcacg cgcgcacgcg cgcgctctcg
     1201 ctcgattacg acagatccgt caaatcatca ttcgtccacg ccacatcgct ccacggcgac
     1261 gagtcgccat cgtcgtcgtc gccgccatcg tcatccgagc cgcgatggtg gcggtcgatg
     1321 tcgtcatccg tcaccttgct gtaatcgata tgcgcatccg tctgcagccc caggctttgg
     1381 ctggcgtcgt cgggcgtgtt gctgaagtcc ggcagactgc cggtgacgag ttgcgtggca
     1441 tccagcggcg tcgttgcgat gctggccgcg ggggcggcga gcgcggacat cgcaatgctg
     1501 acggccgcgg ggccggtggc ggtttggccg gggagttgga acgcgcgcac gtaatcgatc
     1561 tgcatcgtcg cggggatcgg cgtgccgctg tcgggggcgc cgggccagct gttggcgtcg
     1621 ccgacggcca ggttcaggat gatgtattcg gccacgttcg gcacgttgct ggtgatcgtg
     1681 tagtacggtt tgccgtcgaa gtaccacgtc atgtgatcgg gctgccagtc caggccgtac
     1741 gtgtggaacc cggcggacag atcgaggccg gtgtcgaagc ttttgccttg cgtgccgttg
     1801 tgatgcagat gggcttcggt gcgggtcgga tcggtgccga cctgttccat gatgtcgatc
     1861 tcgccgtcgc catcgtgata gttgccgttg gcatcgggcg tgggcatcat ccagaacgcg
     1921 ggccaaagac cggagccctt gttgaggcgc atgcgggctt cgaggtagcc gtacttgatg
     1981 gagaagccgg ggagattctt gcccacggtg gcgaagtccc cgccggtgtc gatgagaccg
     2041 ctggtgtagg ggaagccgtt ggatgattcc ttgcgggcgg tgaggttgag gttcccgccg
     2101 ctgacgttga cgttgttcgg cgtgtagttc tcgatctcgc cgctgaagtg ggtggtgccc
     2161 cagagggtgt ctacccaggt gggcgtcgct ggggtcgcgt tgaattcgtc ggcgaacttt
     2221 agagaccagt tcccgccgac cggcggcggg gtcacgcccg tcggctgcgg ctgcggctgc
     2281 ggctgcggct gcggctgtgg attgggttga ggctggggtt gtggatccgg ctgtggctgc
     2341 ggattcggtt gcggctgtgg atcaggattc ggtggtgtga ccgttggcga accatcggtg
     2401 acgaccagct gcaccgacac ccgcgagccg cgcgtgccgg tcgccttggc ggcgctgtac
     2461 ggcgtggcgg tcagcttgtg tgtgcccgtg tccggcgtcc acgcgtagta accacctttg
     2521 ccattgtcgc cggccagatc gaacggcgcg acattctcaa tgtgatatcg gctgacgccg
     2581 tcgtatccga agcgcacgct gctcgtaccc acccccgtgt cggcgcgcac gttcaaattc
     2641 cgcgtcggca gcgtcgatag gtccagcggc acccgcccgc tgagcggcat gatgtcctgc
     2701 cccgtgtcgg cgttcaccag caccagtccg ctcacgctga ccgacagcag ttgtcgccgc
     2761 tccagcggtt cgaaaatgct cggcgatttc ggatgaaccg ccgatgcccg aagcgagttg
     2821 cgatgacgac gccgattacg attgcccgcc cgatgacctg agcccttacc catgatgcga
     2881 cctccaaaag ttgagacacc tcgacatcgg ccggcgcgcc cgtcgagcgc ggagcatctg
     2941 cgcaccgggt gcgcgcagac cgtcagcccg gccattaaca cagttcaaaa tttgcgatta
     3001 cgaacccgcg attggagaac agcggcgccg ctgcgtgaac ggaagtgggg tgcgccgatg
     3061 gcctacgcgg tttgcacgaa tgccgggtgc ggatgcgaac ccacgccgct cgactcggcc
     3121 tgacctaacg acgcggtcac ccacgggtca gaaaaatcga ttccagcatt cgatgctctt
     3181 gcccgaaacg atccagcgca cacaaggatc gcaaccagcc cgtcccagcg ctcgcggcgc
     3241 cgccaaagat cactcagcgg cgcccacgct tgggacagat gtgcaaccta aggttcagcc
     3301 aataacggtc aacccgaccg cgcgttcaaa atcttatttt tttgcgatcg tcgcagatgc
     3361 cgcgattttt cgctgaaaaa ccgccccaaa agtggtgatg gcaaggtgac ggggatttgc
     3421 gggctggccg cgccaccgtc acaggcgttg gcggcttgat ttttccccct ctcccggttt
     3481 acccggcaat ggaccagcgc gcgcggcgcc tcaggttttt gcacgggctg agcaccgcgg
     3541 cacggggtat ggtataatga cttgcgatgg atcgggctgg gaagcgtatc ttattagttg
     3601 aagaccatgc cgacacgctg gcgacattcg atcgcctgct gggccggtgg ggctacgccg
     3661 tctgcacggc cagctgtgcc gccagcgcca gcgccgccat cgaacgcgaa cgcttcgacc
     3721 tgctgatctg cgacatcggc ctccccgacc aaccgggcac cagcgtgatg cgcgcgatgc
     3781 gcgccgcgaa ccccaccgtg ccgggcatcg cgctgaccgg atacggcatg gaaggcgaga
     3841 tcgaaacgat catggccgcg gggttcacgc ggcacctgct taagccggtc gagatgcaga
     3901 tcctgcaaac cgccatcgaa gacgtgctga agcagcgcac gccggccatc acccaggtca
     3961 ccggcggttg ccgcccggcg atttgaccgc cacgtccgcc agtcgatagc gttggcgttc
     4021 atgccaacca gccgatccgc cgctccaatc gtttattgct tcatcgcgat tttcgtctcg
     4081 ctcttcatgc cgggttctgg gcaggctcaa gagaaggtga aggtcgagat ccgcgtcacg
     4141 gtcccgaagg aaacaccggc cgattcgacg atctacatcg ccggcgatca accggccgtg
     4201 ggccaatgga aggccgacgg cgccgccctt aaaccggccg gagatcacgt ctactcgatc
     4261 cagctggagc tgcccaaggg cggcacgatg gaatacaaga tcgaccgcgg atcgtgggag
     4321 acggtggaga aggacgccaa gggcgacgag gtcggcaacc ggacgctgtc gctggacggc
     4381 gacaagacgg agaatatcac cgtcgccacc tgggcgggcg ccaccgcggc gggcggcgcg
     4441 gacaaaccag cggtgccggc cgcgccggcg gcgcccaagg cctccggcgt cgtcggtgac
     4501 gtgagatacc accaatcgtt ccactccgac attctgcaca acgaccgccg gatcatcgtc
     4561 tggctgccgc cgacgtattc gaccgacaca tccgcgcgct tcccggtgct ctacctgcac
     4621 gatggccaga actgcttcga cgcgagtacc tcgtacgccg gggaatggcg cgccgatgag
     4681 acggctgatg cgctgatccg agcgaataag atgaagccga tcatcatggt cgccgtcgcc
     4741 aacgccggtc cggatcggat gaacgagtac acgccgacgc gcgacgccgt ccgcaacgcc
     4801 ggtggacgcg gcgagcagta cgcgcagttc ctgatcaccg aggtcaagcc gttcatcgac
     4861 cgcacgtatc gcacgcaagc cgaccgcgaa aacaccgccg ctgccgggtc atccctcggc
     4921 ggcctcatct cgttgtacct ggcgtacaag cacggcgaca tcttcggcgc cgccggcgtc
     4981 atctcgccat cgctcagttg ggacaacgct tcgctgatga aacagatcga agccgacccg
     5041 tcgccgctga aggccgagcg catctggctc gacatgggcg gcgacgaagg caaccctacc
     5101 gaagccgccc agggcgtggc caacgccaag tccctcgcgg cgacgctgca ggggacgatc
     5161 ggcgagaaga acttgaagta tctggaagtc cccggcgcca agcacaacga agccgcctgg
     5221 gcggcgcggt tcgatcaggt gttggtgttt ttgtttggga cgtaaggcca gtgcagtgga
     5281 tcgatccatc caatcccggt gcaacttccg tagcccccca cgccgggttc ggaggtactc
     5341 cgacgacc
//
LOCUS       DATIMH010000176         5193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4081947, whole genome shotgun sequence.
ACCESSION   DATIMH010000176 DATIMH010000000
VERSION     DATIMH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4081947"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VLI90_02945"
     CDS             complement(<1..196)
                     /locus_tag="VLI90_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13187.1"
                     /translation="MRKDLALASCILAGLLAVGCDSKSNDTTTSTTPPAAPAAPPQST
                     GNAVSDGVKNATGSVTNAANN"
     gene            446..1537
                     /locus_tag="VLI90_02950"
     CDS             446..1537
                     /locus_tag="VLI90_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HSV13188.1"
                     /translation="MAINYIVPPKVHNELVAAAYRHRGYTADEAEAGARFSELTAVHG
                     IKTHNAIKALHLDELFGSKSGGCKPGAKIDRLPTKYKAVQKWNANRKLGQATAFEAMD
                     TAMNLADEFGVGVVAVDNAFHYLWGGGYVIDAARKGYISYTSCTAALAEVVPFGGKFP
                     TLGTNPHSWGFPTQDVIGFPICIDWATSVVAMGRIQQFIREGKPVPTGWGVDELGNET
                     TDPSKIKALFPFGQHKGYGLSLIDELYAAYMGGSLPTIRNRWDKVPEGEKGTCCFYFQ
                     CIKPDAIAIDFANKRTQNENVKAVIRDIIGHGNEKAMLPGQIEANGAALSRKHGGLLF
                     TEAEVKALGEIAHEAGVRFDAAAFKTVDL"
     gene            1620..2408
                     /locus_tag="VLI90_02955"
     CDS             1620..2408
                     /locus_tag="VLI90_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV13189.1"
                     /translation="MAFYPGLDITNKVCLVTGGTSGIGRAIALGFAKAGAKVIAGSTS
                     ADKVGAIAKELGDGHHALQINVADEASVAQAFAAAKEKYGRIDAVVNAAGVIKRQPSL
                     EMPVSEFRRIVEINLTGTFIVDQAAGRIMKDQSPAANGERGCIVNIASLNSFISLTEV
                     LAYASSKSGVMGVTRGLANEWAQYGIRVNGIAPGVFPTDLNRALIEGTPRGKFLKDHT
                     PQGRFGSADELVGAAIYLISPAASYTNGETIIVDGGFLTRGVGV"
     gene            2565..3401
                     /locus_tag="VLI90_02960"
     CDS             2565..3401
                     /locus_tag="VLI90_02960"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSV13190.1"
                     /translation="MIIDAHHHFWRHNDREFAWISDAMSIIRRDFLPGDLQQAIGAAG
                     VDGVISVQARQTLDETNWLLDLADQNAFIQGVVGWVPLVSPTVGDDLERFAQRAKLRA
                     VRHVLHDEPDDDYCLRDDFNRGISQLRRFNLAYDVLVFERHLPQAIQFVDRHPEQVFV
                     VDHVAKPKIKDNEMEPWRTKLRELARRPNVYCKMSGMVTEADCQAWTPQQLRPYADVV
                     LEAFGPTRVMFGSDWPVCLIACGYARWREVVMGFISKLSPTEQASVMGGTAQRAYALT
                     GG"
     gene            complement(3665..4849)
                     /locus_tag="VLI90_02965"
     CDS             complement(3665..4849)
                     /locus_tag="VLI90_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HSV13191.1"
                     /translation="MAKKTVADVDVRGKRVLMRADFNVPLEGSRITDDRRIAQAMPTI
                     KSIIERGGRLILMSHLGRPKGGPEAKYSLKPVADRLGELLHKPVKFANDCIGPDVDKL
                     VADLKDGDVLLLENLRFHKEEEKNDPNFAKQLAKAGDVYVNDAFGTAHREHASTFGVV
                     QALAGKPRVIGFLIQKELKFLGEAISNPKRPFVAILGGAKVSDKINVIEQLLGKADTL
                     LIGGAMAYTFFLAQGKEVGKSLVERDKVDLAKTLLAKAGGKIKLPVDTVISDKMTDDA
                     QTRIVEGNIPADMEGFDIGPKTRELYKAEVAKAKTIVWNGPMGVFEKKPFAAGTQAIA
                     QAVADATAKSGAVSIIGGGDSAAAIEQMGLAEKVSHVSTGGGASLEFLENGHFSTLDI
                     LD"
     gene            4935..>5193
                     /locus_tag="VLI90_02970"
     CDS             4935..>5193
                     /locus_tag="VLI90_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13192.1"
                     /translation="MIHGKRRDDHRVTMEDAVGHGHRMGVDRPSAAAQHEPRFHVVLS
                     ARLGNASSRSHELARRGKKTTDGYGVYTHRSRAALARGPLGF"
BASE COUNT          988 a         1660 c         1600 g          945 t
ORIGIN      
        1 cgttgttggc ggcgttggtc acgctgcccg tcgcgttttt cacaccatcg gaaacggcgt
       61 tgccggtgct ctgcggcggc gccgccggcg ccgccggtgg cgtggttgat gtcgtcgtat
      121 cgttcgattt gctgtcgcac ccgacggcca gaagaccggc gagaatgcac gacgccaatg
      181 ccagatcctt ccgcataaat cgctcctttc gcaaacatcc cccgtcgggg tgggatggta
      241 gcactcgcct aagattttcg caccagtcaa tccggattca cgacaactgc agcgcgccat
      301 agctaattca aaccgtcgga gccccgcatg aacatgcgag gcttctacat cggcttctgc
      361 atcgcgttgc cgcgcttggc atcccccgcc gctgccggta atctgctccg tcccatttac
      421 agtttcaagc ggagttcgga cagacatggc catcaattac atcgttccgc cgaaggttca
      481 caacgagctg gtcgccgccg cgtatcggca ccggggttac accgccgacg aagccgaggc
      541 cggggcgcgc ttcagcgaac tgaccgcggt gcacggcatc aagacgcaca acgcgatcaa
      601 ggccctgcac ctggatgagt tgttcggttc aaagtccggc gggtgcaagc ctggggcgaa
      661 gatcgaccgc ctgccgacaa agtacaaagc ggtgcagaag tggaacgcga accgaaagct
      721 gggccaggcg acggcatttg aggcgatgga cacggcgatg aatctggccg acgaattcgg
      781 cgtcggcgtc gtcgcggtgg acaacgcgtt ccattatctc tggggcggcg gatacgtcat
      841 cgacgcggcg cgcaaagggt acatctcata tacatcgtgt accgcggcgc tcgcggaagt
      901 ggtgccgttc ggcggtaagt tcccgacgct cggcaccaac ccgcacagct gggggttccc
      961 cacacaggac gtgatcggct tcccgatctg catcgactgg gcgaccagcg ttgtcgcgat
     1021 gggccgcatc cagcagttca tccgcgaagg caagccggtg ccaaccggct ggggtgtgga
     1081 cgagctcggc aacgagacga ccgacccgag caagatcaaa gcgctgttcc ccttcggcca
     1141 gcacaagggt tacggcctgt cgctgatcga tgaactgtac gccgcgtata tgggcgggtc
     1201 attgccgacc atccgcaacc gctgggacaa ggtgcccgaa ggcgaaaagg gaacttgctg
     1261 cttttacttc caatgcatta aacccgacgc gatcgcgatc gatttcgcaa acaagcgaac
     1321 gcagaacgag aacgtcaaag ccgtcatccg cgatatcatc ggtcacggca acgaaaaggc
     1381 gatgctgccg ggacagatcg aggcaaacgg cgccgctttg agtcgaaaac acggcggact
     1441 gctattcaca gaagcggaag tgaaagcgct gggcgagatc gcccacgaag ccggcgtaag
     1501 gttcgacgcg gcggcgttca agactgttga tctataagta gcatgggcag cctgctcatg
     1561 aaccgccgct ataggggcaa aacacgggca agttgcccgt gccacgagtg gagaagcata
     1621 tggcgttcta tcccggtctc gacatcacca acaaagtctg cctcgtcacc ggcggcacca
     1681 gcggcattgg ccgggccatc gcgctcggct ttgccaaggc cggcgcgaag gtcatcgcgg
     1741 gttcgaccag cgcggacaag gtcggcgcga tcgccaagga attgggcgac ggccatcacg
     1801 cgttgcagat caacgtcgcc gatgaagcca gcgtcgcgca agccttcgcc gccgcgaagg
     1861 agaaatacgg gcggatcgac gccgtcgtca acgccgcggg cgtgatcaag cggcagccgt
     1921 cgctggagat gccggtgtcg gaattccgcc ggatcgtcga gatcaacctg accggcacct
     1981 tcatcgtgga ccaggccgcc gggcggatca tgaaggacca gtcccccgcc gccaacggcg
     2041 aacgcgggtg catcgtcaac atcgcgtcgc tcaacagctt catctcgctg accgaagtgc
     2101 tggcctacgc cagcagcaag agcggcgtga tgggcgtgac ccgcggcctg gccaacgagt
     2161 gggcccaata cggcatccgc gtgaacggca tcgcgcccgg tgtttttccg acggacttga
     2221 accgcgcgct gatcgaaggc acgccgcgcg gcaagttcct caaggaccac acaccgcaag
     2281 gccgcttcgg cagcgcggac gaattggtcg gcgccgcgat ttacctgatc agccccgccg
     2341 ccagctacac caacggcgag acgatcatcg tcgacggcgg attcctgacc cgcggggttg
     2401 gcgtctaggc acaaccgccg acgtaaagga tcggccgccg tgacaccggc ctccggcccg
     2461 tgcgcgaggc acgaaacgtc gatcaccgcg ggatcggtca tctgcaagtc tttcgcacgg
     2521 gccggaggcg gtgtcacgat gcgccccgtt cgagttcgcg gctcatgatc atcgacgccc
     2581 accatcattt ctggcgccat aacgaccgcg aattcgcctg gatctccgac gcgatgtcga
     2641 tcatccgccg cgactttctg ccgggcgatc tgcagcaggc gatcggcgcc gccggcgttg
     2701 atggcgtcat ctccgttcag gcgcgccaga cattggacga gacgaattgg ctgctcgacc
     2761 tggccgatca aaacgcgttc atccagggcg tcgtcgggtg ggtgccgctc gtttctccga
     2821 cagtcggcga tgatctcgag cgtttcgcgc agcgcgcgaa actgcgagcg gtccgacatg
     2881 tcctgcacga tgagccggac gacgactact gcttgcgcga tgacttcaac cgcggcattt
     2941 cgcagctgcg gcggtttaac ctcgcgtacg acgtcctggt cttcgagcgg cacctgccgc
     3001 aggcgatcca attcgtcgat cggcatcccg agcaggtgtt tgtcgtggac cacgtcgcca
     3061 agccgaagat caaagacaat gagatggagc cgtggcgaac aaagctgcgc gagctggctc
     3121 gccgaccgaa cgtgtattgc aagatgtccg gcatggtgac cgaggcggat tgccaagcat
     3181 ggacgccgca gcaactgcgg ccctatgcgg acgtggtcct cgaagcgttc ggtcccaccc
     3241 gcgtgatgtt cggctccgat tggcccgtct gcctgatcgc ctgcggctac gcgcgatggc
     3301 gcgaagtggt gatggggttc atctcaaagt tgtcgccaac cgagcaagcc agcgtgatgg
     3361 gcggaacggc gcagcgcgcc tatgcgctga cgggcggttg aaacggcctt gtcattctgg
     3421 gcgaagcacg tactcgtgtg cagtcgaagg atccagccgc gaagtcggag aatcgtcgtt
     3481 tgcgcaaatc agccgcttgc ggcttcgcag atcaggagcc gctgaattgc gaacccgcaa
     3541 gcggctgatt tgcgcgaggg gcggttcact aacccgccgg caagatcctt cgactccgga
     3601 cttcggcgag ctcagtcgag gcgcgagtac gcctgcgctg gaggatgaca gcaccacacc
     3661 cccgctagtc caaaatatcc aacgtcgaaa agtgcccgtt ctccaaaaac tccaacgatg
     3721 ccccgccgcc ggtgctgacg tggctgacct tttccgccaa gcccatctgc tcgattgccg
     3781 cggcgctgtc gccgccgccg atgatgctga cggcgccgct cttggccgtc gcgtcggcca
     3841 ccgcttgcgc gatcgcctgc gtgccggcgg caaaaggttt cttctcgaac acgcccatcg
     3901 ggccgttcca gacgatcgtc ttcgccttgg cgacttcggc cttgtacagc tcgcgcgtct
     3961 tgggcccaat gtcgaagcct tccatgtcgg cggggatgtt gccttcgacg atgcgcgtct
     4021 gcgcgtcatc ggtcatctta tcgctgatga ccgtatccac cggcagcttg atcttcccgc
     4081 cggccttggc gagcagggtc ttggcgagat ccaccttgtc gcgctcgacc aagctcttgc
     4141 cgacttcctt gccctgcgcg aggaagaacg tgtacgccat cgccccgccg atgagcagcg
     4201 tgtcggcttt gccgagcagc tgctcgatga cgttgatctt gtccgacacc ttcgccccac
     4261 ccaggatcgc gacgaacggc cgcttcggat tgctgatggc ctcgccgagg aatttgagtt
     4321 ccttctggat gagaaacccg atgacgcgtg gcttccccgc cagcgcctgc acaacgccaa
     4381 acgtgctggc gtgctcgcga tgagcggtgc cgaacgcatc gttcacatac acgtcgccgg
     4441 ccttagccaa ttgcttggcg aagttgggat cgttcttctc ctcttccttg tggaagcgga
     4501 ggttctccag cagcagcacg tcgccgtcct tcagatcggc gaccagtttg tcgacgtcgg
     4561 ggccgatgca atcattggcg aacttcaccg gcttatgcag cagttccccc agccgatccg
     4621 ccacgggctt cagcgaatac ttcgcctccg gtccaccctt gggccggccc aggtgcgaca
     4681 tcaggatcaa ccgcccgccg cgttcgatga tgctcttgat cgtcggcatc gcctgcgcga
     4741 tccgccgatc gtcggtaatc cggctgccct ccagcggcac gttgaaatcc gcccgcatca
     4801 gcacgcgctt gccccgcaca tccacatccg caacggtttt cttggccatg atcgaattcc
     4861 ttattttgga gcggcgatgc taagacgcgc gagcggcgtg tcaagaacta ttcagatgca
     4921 atacgcgagg cggcatgatc catggcaagc ggcgcgacga ccatcgcgtg acgatggaag
     4981 acgccgtggg tcacggacat cgaatggggg tggatcgacc gtcggccgct gcgcaacatg
     5041 aaccgcgctt tcatgtggtg ctcagcgcga gattgggtaa tgcatcatcg cgttcgcacg
     5101 agctagcccg ccgcggcaag aagaccaccg acggctacgg cgtctacacc catcgcagcc
     5161 gcgcggcttt agcccgcgga ccgttgggat ttc
//
LOCUS       DATIMH010000177         1639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5527496, whole genome shotgun sequence.
ACCESSION   DATIMH010000177 DATIMH010000000
VERSION     DATIMH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5527496"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1557
                     /locus_tag="VLI90_02975"
     CDS             37..1557
                     /locus_tag="VLI90_02975"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HSV13193.1"
                     /translation="MPLLELTGICKRFPGVVALDKVSLSLEKGEVVALIGENGAGKST
                     LMKTLGGIHQPNEGEILIDGKPTVIKSVADSVALGVGFVHQELNNLDNLDVAANLFLG
                     REPLWGGPLKLVDHRAMVEQSKRYLSRLGLNISPKTSLGSLSIAQQQMVEIAKALSQN
                     ARILILDEPTSSLTAAETEKLLQTVRDLRAEGVSVLYISHRLGEIRQIADRVVALRDG
                     KNAGGLSRDQITHDNMVRLMIGRDVKNFYVQPSSTARQGRLSVRNVRTAAKPAHAVSF
                     DAAGGEILGFAGLVGAGRSELMQAIFGVDPMLQGEVLLDGQPLRVGRPRDAINAGIYL
                     VPEDRRKTGLIVDMTIRENITLPALWNYTSAALISRARECEESQKQVKALRVKTPSLE
                     AKAMNLSGGNQQKVVLGKWLSLKPKVLIVDEPTRGIDVGAKAEIYRLLRALSDEGVAV
                     IAVSSDLEEVLNISDRIAVMREGAIAGMLRREQFSEQAVMDLAFGNTNIPSATAAA"
BASE COUNT          319 a          480 c          561 g          279 t
ORIGIN      
        1 ctcatcgttc cccgtacatc gctcatcgtt aacgctatgc ctcttctaga actcaccggc
       61 atctgcaagc gtttccccgg cgtcgttgcc ttggacaagg tctcgttgtc gctggagaag
      121 ggtgaagtcg tcgccctcat cggtgagaac ggcgcgggca agtccacgct catgaagacg
      181 ctgggcggca tccatcagcc gaacgaaggc gagatcctca tcgacggcaa gcccaccgtc
      241 atcaagtccg tcgcggactc ggtggcgctg ggcgttgggt tcgtccacca ggagctgaac
      301 aacctggaca acctggatgt cgccgcgaat ttgttcctcg gccgcgaacc gctgtggggc
      361 gggccgctga agctggtcga tcaccgggcg atggtcgagc agagcaagcg gtatctgtcg
      421 cggctgggat tgaacatctc gcccaagacg tcgctcggat cgctgtcgat tgcgcagcag
      481 cagatggtgg agatcgccaa ggcgttgtcg cagaacgcgc ggattctcat cctcgacgaa
      541 ccgaccagct cactgacggc ggccgagacg gaaaagctgc tgcagaccgt tcgcgacctg
      601 cgggccgagg gcgtcagcgt gctgtacatc tcgcaccggc tgggcgagat ccggcagatc
      661 gccgaccggg tcgtcgcgct gcgcgacggc aagaacgccg gtggtctgtc gcgcgatcag
      721 atcacgcatg acaatatggt ccggctgatg atcggccgcg acgtgaagaa tttttacgtg
      781 cagccgtcga gcaccgcgcg gcaagggcgc ttgagcgtgc ggaacgtgcg caccgctgcc
      841 aagccggcgc acgcggtgag cttcgatgcc gccggtggtg agatacttgg gttcgccggg
      901 ctcgtcggcg cgggccgttc tgaactgatg caggcgatct tcggcgtcga tccgatgctg
      961 cagggtgaag tgttgctcga cgggcagccg ttgcgggttg gacggccgcg cgacgcgatc
     1021 aacgccggga tttacctcgt gcccgaagac cgccgaaaga ccgggctaat cgtggatatg
     1081 acgatccgcg agaacatcac gttgccggcc ctgtggaact acaccagcgc cgccttgatc
     1141 agccgggcgc gcgaatgcga agaatctcaa aaacaggtga aggcgctgcg cgtgaagacg
     1201 ccgtcgctcg aggcaaaggc gatgaacctg agcggcggga accagcagaa ggtggtgctg
     1261 ggcaagtggc tgtcgctgaa accgaaggtg ctgatcgtcg atgagccaac ccgcggcatc
     1321 gacgtgggcg ccaaagcaga aatctatcgg ctgctgcggg cgttgtccga cgaaggcgtg
     1381 gcggtgatcg ccgtctccag cgacctggag gaagtgttga acatcagcga ccgcatcgcg
     1441 gtgatgcgcg aaggcgcgat cgcggggatg ctcaggcgcg agcagttttc ggagcaggcg
     1501 gtgatggacc tggcgtttgg gaacacgaac atcccaagcg ccactgccgc agcgtaagag
     1561 tgagagttga tgcagaagac gaaataaacc tccgtgttta gccgccgcgg cctcgcggcg
     1621 cgaacgtcga aggcgccgc
//
LOCUS       DATIMH010000178         5034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1773249, whole genome shotgun sequence.
ACCESSION   DATIMH010000178 DATIMH010000000
VERSION     DATIMH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773249"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..597
                     /locus_tag="VLI90_02980"
     CDS             55..597
                     /locus_tag="VLI90_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSV13194.1"
                     /translation="MPVNNISIEQCRRLIDSGQTLNILDVRTPAEFCQLHATGARLLP
                     LDELNPAAIAAQPRNDDEPLYVICHSGGRATEACRRLADAGASHVYCIEGGTAAWERA
                     GLPVQRGSIRVMSLERQVRVAAGSLVLLSVALGWFVHPAFLALTAFIGAGLTFAGITD
                     TCGMAMLLAKLPWNRAQACS"
     gene            625..1815
                     /locus_tag="VLI90_02985"
     CDS             625..1815
                     /locus_tag="VLI90_02985"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HSV13195.1"
                     /translation="MAVQLPIYMDHNATTPVDERVFEAMRPYFTRVFGNAASLGHSFG
                     WEADAAVYKAREQVAALLNARPDAIVWTSGATESNNLAVKGAARAQVHRGRHIITQVT
                     EHKAVLDTCKYLQTQGFDVTFLPVDGRGRIDLDQLRAAFRRDTILVSIMWANNEIGTI
                     QEIRTIGAMCRERGILFHTDATQAVAKVPIDVDADNVDLLSMSGHKMYGPKGCGCLYV
                     RNKPQRIDLTPVLHGGGHERGYRSGTLNVPGIVGVGAACEIAKNEMADELPRVRALRD
                     RLQDGILRRVSGTHVVVNGYEPNRLPHMTNLAFEGIDHDQLLFALDGIAVSSGSACTS
                     ASLEPSYVLTALGLPPRLAFPSLRFALGRWTSAEEVDYVIDRLDAALNALRSGDLPPM
                     PLKA"
     gene            complement(1825..>5034)
                     /locus_tag="VLI90_02990"
     CDS             complement(1825..>5034)
                     /locus_tag="VLI90_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13196.1"
                     /translation="SAWSSWSTSNWTGSADVNPVADRLTAGDFYILDGAHLYLSTNGG
                     VSWTLKSSSAPSGTLEASFAAAGDLWISANGNGLWHSTNFGATWTHVASAAITNSSSV
                     GFGKAAAGQTYPAIYVTGSGNGTTGMFRSDDGGTTWLTISDSQHQFSGAIVGDRNIYG
                     RVYIGAGARGVIYGDIQSTAPSAATAAAASPVVGNTSNLSVLGSDDGGESNLTYTWRV
                     TNKPPGSTLPTFSTNGTNAAKNTVATFAQPGFYSFQVALTDAQGHVMISSVGLTVFVS
                     IGAFNSDLDIGSPSPTGSSSYNAGTGTYTVAGGGSDIWNTSDQFHFLSKSFTGDGVMI
                     ARVTSVQNTNTWAKAGVMFRDSTAANSMFADVLVSYSSGVTFQWRNSTGGTADYALIS
                     GIAAPAWVKLVRGGNSFTAFYATTAGTPAAGNWIQIGTPQAIAMGSGIQAGLAVTSHA
                     NGTLCTSTFTGVLVNQNPTVATAASATPNPANSTTSALSALGADDGGEANLAYTWSTT
                     GAPPAAVSFSANGTNAAKNTTATFTKAGTYSFLVTVTDALGLATTSAVSVVVNQSPTL
                     VTVAPNPVTVAGGATKQFSATVTDQFGQPIVSPSVTWSTSGGGSTDTSGLFTASQTGG
                     AFNVTAKSGSASGSAAVNVVPSIYTGTASADNYYVRLSADGVTEQLWVGSTTGAGVPT
                     YTIAQSQLTSLAFNGMGGADSLTVDFTNGNPVPSSGVSFDGGSGSTGNLLVVDGSGSA
                     DTATLSASSVVFNSVAPIAYANTQSLTLATAGGNDVVTQAAQPAVPNVVFNGGSGSDT
                     LNVNAGAFSFNTDAGAGTSSLTVNVAGNGSSASFSGDQHLAGLTLTSSAVVVVTAAAS
                     DLSPAIVNVTALGFDASAGAKLDLVNNELITGDSLSLVRSLIVQGHLWTSIAGRAVGS
                     SDMGGGQVEARPTLQGDANLDGSVNVGDLGSLATHYGTSSGAVWTDGDFDYNGAVDVA
                     DLGYLASGFAASLGGAAVPAAMAASISATDAPATSIPTQKSDAAKPTRSTSFTFATGS
                     SLGDLTTPRESIADLTDHHHGRHLYDSFWRSESEPLDPA"
BASE COUNT          870 a         1556 c         1693 g          915 t
ORIGIN      
        1 tcggcgcgct cggcaccctc ggcgttgcat tgaatttgaa gtgagaggcc catcatgccc
       61 gtcaacaata tttccatcga acaatgccgc cggctgatcg acagcgggca gacgctcaac
      121 atcctggacg tccgcacgcc cgccgagttt tgtcaattgc atgcgaccgg cgcgagactg
      181 cttcccctgg acgaactgaa ccccgcggcg attgccgcac aacctcgaaa cgatgatgag
      241 ccgctgtatg tcatctgcca ctccggcggc cgcgcgaccg aagcgtgccg tcgcctggcg
      301 gatgctggcg catcgcatgt ctactgcatc gaaggcggca ccgccgcctg ggagcgggcc
      361 ggcctgccgg tgcagcgcgg ctccataaga gtgatgtcgc tcgagcggca ggtgcgcgtc
      421 gcggcaggct cgctcgtgct gctgagcgtc gcgctggggt ggttcgttca tcccgcgttt
      481 ttggcgctca cggctttcat tggagccggt ctgaccttcg ccggaattac cgatacctgc
      541 ggaatggcga tgctgctggc gaaactgccg tggaatcgcg cgcaggcctg ttcttgagct
      601 gccgttgacg cgacaataga ggacatggca gtccagttac cgatttacat ggatcacaac
      661 gccaccacgc cagtggacga acgcgtgttc gaagcgatgc ggccctactt cacgcgcgtg
      721 ttcggcaacg ccgcttcact ggggcacagc ttcgggtggg aggcggatgc cgcggtgtat
      781 aaggcgcgcg agcaggtcgc ggcgttgttg aacgcgcggc ccgacgccat cgtctggacc
      841 agcggcgcca ccgaatcgaa caaccttgcc gtcaaaggcg ccgcccgtgc tcaggtgcac
      901 cgcgggcgtc acatcatcac gcaggtgacc gagcacaagg cggtgctcga cacctgcaaa
      961 tatctccaga cccagggctt cgacgtcacg ttcctgccgg tcgatggccg cgggcggatc
     1021 gacctcgatc agctgcgcgc ggcctttcgc cgtgacacga tcctcgtgtc gatcatgtgg
     1081 gccaacaacg agatcggcac gattcaggaa atccgcacga tcggcgcgat gtgccgcgag
     1141 cgcggcatcc tgttccacac cgacgccacg caggccgtcg cgaaggtgcc gatcgatgtc
     1201 gatgccgaca acgtcgatct gttgtcgatg agcggccaca agatgtacgg ccccaagggc
     1261 tgcggctgcc tgtacgtccg caacaaaccc cagcgcatcg acctgacgcc ggtgctgcac
     1321 ggcggcggac acgaacgcgg gtaccgcagc ggcaccttga acgtccccgg catcgttggc
     1381 gtcggcgccg cgtgcgagat cgccaagaac gaaatggcgg atgaactgcc gcgcgtccgc
     1441 gcgctgcgcg atcgcttgca ggatggcatc ctgcgaaggg tgtccggcac ccacgtcgtg
     1501 gtgaacggct acgagccaaa ccggctgccg cacatgacga acctggcgtt cgaaggcatc
     1561 gatcacgacc agttgctgtt cgcgctggac ggcatcgccg tcagttccgg ctccgcctgc
     1621 accagcgcca gtctcgaacc ctcgtatgtg ttgaccgcgc tgggcctgcc gccacgcctg
     1681 gcgtttccct cgctgcgctt cgcgctgggc cggtggactt cggcagagga agtggattac
     1741 gtcatcgacc ggctcgacgc cgcgctcaat gcgctgcgtt cgggtgattt accgccaatg
     1801 ccgctgaagg cgtgaagcca aaagtcaggc ggggtccaat ggctccgact ccgagcgcca
     1861 aaacgaatcg tacagatgcc ttccgtggtg gtggtcggtg agatctgcga tcgactccct
     1921 tggcgtggtc aaatcgccca gggaagagcc tgtggcgaag gtgaacgacg tcgagcgagt
     1981 cggtttcgcc gcatccgact tctgtgttgg aatgctcgtc gcgggcgcgt cggtggcgga
     2041 gatcgacgct gccatggcgg cgggcactgc ggcgccgcca aggctcgcgg caaaaccgct
     2101 ggcaagatat cctaagtctg ccacgtccac cgctccgttg tagtcgaagt ccccgtcggt
     2161 ccagacggcg ccgctgctcg tgccgtagtg ggtggccagg ctgcccaggt caccgacatt
     2221 cacgcttccg tcgagatttg catcgccctg caaggtcggc cgggcttcga cctgtccgcc
     2281 acccatgtcg ctcgagccga ccgcgcggcc ggcgatagac gtccacaggt gtccctgcac
     2341 gatcaggctg cgcacgagcg acaatgaatc gccggtgatc aattcattgt tcacgaggtc
     2401 gagtttcgcg ccggcgctgg cgtcgaagcc cagcgcggtg acgttcacga tcgccggaga
     2461 cagatccgac gccgcggcgg tgacgaccac cacggccgac gatgtgaggg tcagcccggc
     2521 caggtgctga tctccgctga agctcgcgga agaaccgttg ccggccacgt tgaccgtaag
     2581 actgctggtg ccggcgccgg cgtcggtgtt gaacgagaac gcgccggcgt tgacgttcag
     2641 cgtgtcgctg ccgctgccgc cgttgaacac aacgttcgga acagcgggct gggcggcctg
     2701 cgtcaccacg tcatttccgc cggcggtagc gagcgtgagc gactgcgtgt tggcgtacgc
     2761 aatcggcgca acgctgttga agacgacaga actggcgctg agggtggcgg tgtcggcgct
     2821 gccgctgccg tcgacgacga gcaggttgcc cgtgctgccc gagccgccgt cgaaactgac
     2881 gccgctcgat ggaactggat tgccgttggt gaaatcgacg gtgagcgaat ccgccccgcc
     2941 catgccgttg aaagcgaggc tcgtcaattg ggactgggcg atggtgtaag tgggaacacc
     3001 ggcgccggtg gtggaaccga cccagagctg ttcggtcacc ccatcggcgg acaggcgaac
     3061 gtaataattg tcggccgacg cggtgcccgt gtagattgac ggcaccacgt tcaccgccgc
     3121 actgccgctg gcggagccgc tcttggcggt cacgttgaac gcgccgccgg tctggctcgc
     3181 cgtgaagagg ccggatgtgt ctgtgcttcc cccgccgctg gttgaccacg tgacactcgg
     3241 ggagacgatc ggttggccga attgatcggt caccgtggcg ctgaattgtt tggtcgcccc
     3301 accggcgacg gtgacgggat tgggggcaac ggtgacgagc gttggcgact ggttcacgac
     3361 gaccgagacg gcgctcgtcg tagccaagcc caaagcatca gtcacggtga cgaggaagct
     3421 gtacgtaccg gctttggtga acgtggcggt cgtgttcttc gcggcgttgg tgccgttggc
     3481 gctgaagctg accgcggccg gtggtgcgcc ggtggtgctc cacgtgtaag cgaggttcgc
     3541 ctcgccgccg tcatcggcgc cgagggcgct caaggcggac gtggtgctgt tggctggatt
     3601 gggcgtcgcc gaagcggccg tcgcgaccgt cgggttttga ttgaccaaca cgccggtgaa
     3661 ggtgctggtg catagcgtcc cgttggcatg gctggtgacg gcaaggcccg cctggatgcc
     3721 gctgcccatc gcgatcgcct gcggcgtccc gatctgaatc caattcccgg cggccggcgt
     3781 tcccgcggtc gtggcataga aggcagtgaa gctgttgccg ccgcgcacca gcttcaccca
     3841 cgcgggagcc gcgatgccag aaatcagcgc gtagtcagca gttccgccgg tgctgttgcg
     3901 ccactggaaa gtgactcccg atgagtagct gaccaagaca tcggcgaaca tcgagttggc
     3961 ggccgtggaa tcgcggaaca tcacgccggc cttggcccag gtattggtgt tctggacgct
     4021 ggtgacgcgg gcgatcatca cgccgtcacc ggtgaaactt ttcgacagaa agtggaactg
     4081 gtcggaggtg ttccagatat cgctgccacc gccggccacg gtgtacgtgc cggtgccggc
     4141 gttgtagctg ctggagccgg tcgggctggg cgagccgata tcgagatcgc tgttgaaggc
     4201 gccgatcgag acgaaaaccg tcagaccgac ggagctgatc atgacgtggc cttgcgcatc
     4261 ggtgagggcc acctggaaac tatagaatcc gggctgagcg aaggtggcaa cggtgttctt
     4321 ggcggcattc gtcccattcg tggaaaatgt gggcagagtt gagccgggcg gtttattcgt
     4381 cacgcgccag gtgtaagtca gattggattc cccgccatcg tcactgccca gaacactgag
     4441 gttgctcgtg ttgccgacca ccgggctggc ggcagcggcg gtggcggccg aaggggcggt
     4501 agattgaatg tcgccataaa tgacgccgcg cgcgccagcg ccgatgtaga cgcgaccata
     4561 gatgttgcgg tcgccgacga tggccccgct gaactgatgc tgggaatcgc tgatggtcag
     4621 ccacgtggtt cccccgtcat cgctgcggaa catgccggtg gtgccgttgc cggaaccggt
     4681 cacgtatatc gccggataag tctgccccgc ggcggctttg ccgaatccga cgctgcttga
     4741 gttggtgatc gccgcgctgg cgacatgcgt ccaggttgcg ccgaagttcg tcgaatgcca
     4801 cagtccattg ccgttggcgc tgatccacag atcacccgcc gcggcgaatg aagcttctag
     4861 cgtgcccgaa ggcgcggagg atgatttaag cgtccagctc acgccgccat ttgtacttaa
     4921 atatagatgg gcgccgtcga gaatatagaa gtcaccggcg gtcaagcggt cggcgacggg
     4981 gttcacgtcg gccgagccgg tccaattgga ggtgctccaa ctgctccagg cgct
//
LOCUS       DATIMH010000179         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4370695, whole genome shotgun sequence.
ACCESSION   DATIMH010000179 DATIMH010000000
VERSION     DATIMH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4370695"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /gene="thiC"
                     /locus_tag="VLI90_02995"
     CDS             complement(<1..1144)
                     /gene="thiC"
                     /locus_tag="VLI90_02995"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase"
                     /protein_id="HSV13197.1"
                     /translation="MAPAPCTQESEMRTEWVEKRKNDKIRTQMHYARQGVITEEMHYV
                     AKRERITPELVRSEVARGRMIIPANIHHANLEPMCIGVASTCKINSNIGNSSVLSDIE
                     GELDKLRYCIKYGADTVMDLSTGGDIPRIRKSIIEASPVPIGTVPIYEALSRVRRVED
                     LNINVMLEVIEEQAEQGVDYMTIHAGVLVQYIPMTTKRVTGIVSRGGSILAEWMVKNH
                     KQNMLYECFEDICKIFQKHDVSFSLGDGLRPGSVADASDEAQFAELKTLGELTKKAWE
                     YDVQVMIEGPGHIPMDEIEMQVLKEKEMCYEAPFYTLGPLVTDIAPGYDHITSAIGAA
                     MIGWYGASMLCYVTPKEHLGLPNREDVKAGIIAYKIAAHAADIGRKR"
     gene            complement(1302..>1580)
                     /locus_tag="VLI90_03000"
     CDS             complement(1302..>1580)
                     /locus_tag="VLI90_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HSV13198.1"
                     /translation="DFTTKDIGMDIAIEQVRVADHSDAAAKSIKDLQLGRDFGVIVMA
                     IRTADGRMLFNPHADTKVNGGDFLIVMGRQENLRTLESLLAEPRAARG"
BASE COUNT          257 a          498 c          454 g          371 t
ORIGIN      
        1 gacgcttgcg gccgatatcc gccgcgtgcg ccgcgatctt gtacgcgata atccccgcct
       61 tcacatcctc gcgattcgga agcccgaggt gctccttggg ggtcacatag cacagcatcg
      121 acgccccgta ccagccgatc atcgccgcgc cgatcgcgga ggtgatgtgg tcatagcccg
      181 gcgcgatatc ggtgacgagg ggtcccagtg tgtagaaggg cgcctcgtag cacatctcct
      241 tttccttcag gacctgcatc tcgatctcgt ccatcggaat gtgcccgggc ccttcgatca
      301 tcacctggac atcgtattcc cacgccttct tcgtcagctc gccgagcgtc ttcagttcgg
      361 cgaactgcgc ctcgtcgctc gcatccgcga cgctgccggg gcggagtccg tctcccagag
      421 agaaactcac gtcgtgcttc tggaagatct tgcaaatgtc ctcgaagcac tcgtacagca
      481 tgttctgctt atggttcttc accatccact cggcgaggat cgacccgccg cggctcacga
      541 ttccggtgac gcgcttggtg gtcatcggga tgtactgcac cagcacgcct gcatggatcg
      601 tcatgtagtc gacgccctgc tcggcctgct cctcgatgac ctcgagcatc acattgatgt
      661 tcaggtcttc cacccgccgc acgcggctga gagcctcgta gatcggcaca gtgccgatcg
      721 gcacgggaga cgcttcgatg atggacttgc ggatgcgcgg gatatcgccg ccggtggaga
      781 gatccatgac ggtgtcggcg ccgtatttga tgcagtagcg cagcttatcg agctcgcctt
      841 cgatgtccga caaaaccgaa gagttcccga tgtttgaatt gatcttgcac gtggaggcga
      901 cgccgatgca catgggctcc agattggcgt gatggatgtt cgccgggatg atcatgcgcc
      961 cgcgagccac ttccgagcgg accagttccg gtgtaatccg ttcgcgtttc gctacgtaat
     1021 gcatctcttc ggtgatgacg ccctgccggg catagtgcat ttgcgtgcga attttgtcgt
     1081 ttttccgttt ttcaacccac tctgtgcgca tctctgactc ctgtgtgcag ggggccgggg
     1141 ccactcatcc attcacggac gccccggccc ggctgggacc tgagggactc ttacttcttc
     1201 tttttcggct tcttgacttc tttcttgggg cttctgtcct tcgccactag atcctccctt
     1261 acagatgaga ttataaccgc ttgcggagga tcggccccga attatccgcg agccgcgcgt
     1321 ggttcggcga gcaggctttc gagggtccgc aggttttcct gccggcccat cacgatcagg
     1381 aagtcgcccc cgttcacttt cgtgtcggca tgcggattga acagcatccg gccatcggcc
     1441 gtgcggatgg ccatgacgat caccccgaaa tcgcgcccga gctgcaggtc cttgatgctc
     1501 ttcgccgcgg cgtcgctatg gtcggccacg cgcacctgct cgatcgcgat gtccatgccg
     1561 atgtctttgg tggtgaaatc
//
LOCUS       DATIMH010000180         4390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_329913, whole genome shotgun sequence.
ACCESSION   DATIMH010000180 DATIMH010000000
VERSION     DATIMH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329913"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1966
                     /locus_tag="VLI90_03005"
     CDS             260..1966
                     /locus_tag="VLI90_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSV13199.1"
                     /translation="MRRKCAAALVAASAMLLSSGARSASALVGSTSNLNINTFLGADR
                     FYNAGYTGSRTIVANIEAGWVWNGQESLTQVATLFQSDFNTGEFDRHATWVGSTIAGR
                     PTAGGGEYQRGIAYGATLWSGSISTGWAPPSPPATYSLGFGFSGGSMAQPYIIAMQTG
                     IGGQTADVINSSWSTGGSPGGNSYSAETLDSMAFATGKTIVVAAGNNGPGPDTVFAPA
                     SGLNSIAVAALTSDVTDPPYNSAASFSSRSPNEFFLPTSPSGITGTVIDNARAAIDIA
                     APGVDLTLAEYDGPTGGNGFGGPSSLANNLYKGGLNGTSFASPVVAGGAALVVDAGKA
                     LFPADPSAIDGRVVKAVLMNSADKTANWNNGQTLANGVLTTTQALDYVTGAGRMNLSR
                     AFDQYTAGTTDLPGVVGGAVQDIGWDFGRVATGAPQDYLLNGTLAAGSMLTATLDWFA
                     DGQTSNYGSFDNLDLQVWSADPNGAPLAMIAQSATLYNTSEHLSFALPGDGQYLLRVE
                     WENNLYNFAGDRPRDNYGLAWNVTAVPEPVSAMGLFAGVFILMRKPSRRGRSSVHDFP
                     PS"
     gene            complement(2005..3510)
                     /locus_tag="VLI90_03010"
     CDS             complement(2005..3510)
                     /locus_tag="VLI90_03010"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HSV13200.1"
                     /translation="MDDRLLKLAEKAKGLPKAPGVYLMKDDKGRVIYVGKSASLRDRV
                     GSYFQPSTKLEHKKVGLLDHVVDFETIQTDSEVEALLAENRLIKDIQPRYNARLLDDK
                     TFPYLMITTRDEFPGVYVTREPLPNTKLYGPFTSVYQLKEAVTLLQKAFKFRTCHLEI
                     SDSDDSRRFFRPCLLYPIKQCTAPCAAKVSKEQYREDINRLIRFLDGDKKRVMAQMEK
                     EMLEASKDMNFERAAKLRDEIKALQGLGARAAKGEQEFWQPEAFITNPREGVAALQEA
                     LQLPEPPRIVECIDIAHLQGDEMVGSKVCFIDGVPFKDGYRRYKIKHGQGNNDYLSIQ
                     EVVSRRYREAGANNELYPDVICIDGGLGQLHAALDVFKTMDVKPPMVISLAKKEELVY
                     VQAKSEPVRLPRNNLGLKLLQYARDEAHRFAQHYHHILRRKSTLEEDVKKGKRPPRKP
                     SERKKAYTEALKTPAPSENPHTTLASLPVLQASEEQEQETIAPLDAEDEAQ"
     gene            3629..3853
                     /locus_tag="VLI90_03015"
     CDS             3629..3853
                     /locus_tag="VLI90_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13201.1"
                     /translation="MFRKLIVTGIITTVVAVLATPASAAGHVRHYTPSRTATVHMSHS
                     NGVAKHHVAKLTKSKKSKGKSHKGGGHKHK"
     gene            4036..4389
                     /gene="bamE"
                     /locus_tag="VLI90_03020"
     CDS             4036..4389
                     /gene="bamE"
                     /locus_tag="VLI90_03020"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HSV13202.1"
                     /translation="MRDQLGFLILIACLIATTGCLFTADSQKTTSGNSVAEGTFSQIT
                     LGKTTVGWVKATLGEPTRRSQADDHEVWEYSYTERTDSSGSVFLIFGGHNSTQTTHKA
                     YVEFKDGVVVNKWRA"
BASE COUNT          746 a         1394 c         1374 g          876 t
ORIGIN      
        1 ttttccatcg cccgatgcga ggcgcatcga cgctcgccac gccgggttcg gaggtactcc
       61 gacgacccgg attttgcggc gcgcgagaag catccgggtc gtcggagtac ctccgagccc
      121 ggcgtggatc ggttcctgac cacttctgca aaagggtcgg accgtcaggg cgtgccgggg
      181 agagggagaa ctcctgcggt ttctcttgcg tactggacca taccccttct gtggttcata
      241 ctcccgttgg aggagagcga tgcgacgaaa atgcgccgcc gcgttggttg cggcttcggc
      301 gatgctgctg agcagcggcg cacggtccgc ctccgcgctc gttggcagca cttccaatct
      361 aaacatcaac accttcctcg gcgccgatcg cttttacaac gcggggtaca ccggttcgcg
      421 cacgatcgtg gccaacatcg aagcgggatg ggtgtggaac gggcaggagt cgctgactca
      481 agtggccacg ctctttcaat ccgacttcaa caccggcgag ttcgaccgcc atgcgacttg
      541 ggtgggttcg acgattgccg gtcggccaac cgcggggggc ggcgaatatc aacgcggcat
      601 cgcgtacggc gcgacgctct ggtccggctc gatctccacc ggctgggcgc caccgtcgcc
      661 gccggcgact tattcgcttg gcttcgggtt cagcggcggg tcaatggcgc agccgtacat
      721 tatcgcaatg cagacgggca tcggcggaca gaccgccgac gtgatcaaca gcagctggag
      781 caccggcggc agccctggtg gcaacagtta ttcagcggag acgctcgatt cgatggcatt
      841 cgccaccggc aagacgatcg tcgtcgccgc ggggaacaac gggccggggc cggacaccgt
      901 gttcgcgccg gcgtcggggt tgaacagcat cgcggtggcg gcgttgacgt cggacgtcac
      961 agatccgccg tacaattcag ccgcgagttt ttccagccga tcgcccaacg agttcttctt
     1021 gccgaccagt ccctcgggca tcaccggcac ggtcatcgac aacgcgcggg ccgcgatcga
     1081 catcgccgcc cccggcgtcg atctcacgct ggcggagtac gacggtccca ccggcggcaa
     1141 cggcttcggc gggccttcga gcctggcgaa caatttgtac aaaggcgggt tgaacggaac
     1201 gagcttcgct tcgcccgtgg tcgccggcgg ggcggcgctg gtcgtcgacg cgggcaaggc
     1261 gctgtttccc gccgatccat ccgccatcga cggtcgcgtg gtcaaagcgg tgctgatgaa
     1321 cagcgccgac aagaccgcca attggaacaa cggacagacg ctggctaacg gcgtgctgac
     1381 gacgacgcag gcgctggatt acgtcaccgg cgcgggccgg atgaatttgt cgcgcgcgtt
     1441 cgatcagtac acggcgggga cgacggatct gcccggcgtt gtcggcgggg cggtgcagga
     1501 catcgggtgg gattttggcc gcgtggccac cggcgcgccg caggattacc tgctgaatgg
     1561 aacgctcgcc gcaggttcca tgctgacggc caccttggat tggtttgccg atggacagac
     1621 gtcgaactac ggcagcttcg acaacctcga tctgcaggtg tggtcggctg atccgaacgg
     1681 tgcgccgctg gcgatgatcg cccagtcggc aacgctgtac aacacgagcg agcacctgtc
     1741 attcgcgctg ccgggtgacg ggcaatacct gctgcgcgtg gagtgggaga acaacctgta
     1801 caacttcgcg ggtgatcggc cgcgggataa ttacggcctg gcgtggaacg tcacggcggt
     1861 gccggaaccg gtgagcgcga tgggtctatt cgcgggggtg ttcatcctca tgcgaaagcc
     1921 ctcgcggcgt ggtcgctcta gcgtccatga ctttccgccc tcgtaaaaac atggtcgcta
     1981 aagcgaccac gccgcgcgtg cgttttattg ggcctcgtct tccgcatcca agggcgcgat
     2041 cgtctcctgc tcttgctcct cgctcgcttg caacacgggt aagctcgcca gcgtcgtgtg
     2101 cgggttctcg ctcggcgccg gcgttttcag cgcctccgta tacgccttct tccgctccga
     2161 cggctttctc ggcgggcgct tgcccttctt cacgtcctct tccagcgttg atttccgccg
     2221 caggatgtgg tggtagtgct gcgcgaaccg atgcgcctcg tcgcgcgcgt attgcagcag
     2281 ctttaagccc aggttattcc gcggcaggcg caccggctcg ctcttggcct gcacgtaaac
     2341 cagctcttcc ttcttcgcca acgagatcac catcggcggc ttcacgtcca tcgtcttgaa
     2401 gacatccagc gccgcgtgca gctggcccag cccgccgtcg atgcagatga cgtcggggta
     2461 cagctcgttg ttggctccgg cttccctgta tcttctacta acgacttctt gaatgctcag
     2521 gtaatcgttg ttgccttggc cgtgcttgat cttgtatcgg cggtagccgt ccttgaacgg
     2581 cacgccgtcg atgaagcaga ctttgctgcc gaccatctcg tcgccctgca ggtgggcgat
     2641 gtcgatgcac tcgacgatcc gcggcggctc gggcagttgc agcgcttcct gcagcgcggc
     2701 cacgccttcg cgcgggttgg tgatgaaggc ctcgggctgc cagaattcct gctcgccttt
     2761 cgccgctcgc gccccgaggc cctgcagcgc cttgatctcg tcgcgcagct tcgccgcccg
     2821 ctcgaagttc atgtccttcg atgcttcgag catctccttt tccatctgcg ccatcacgcg
     2881 tttcttgtcg ccgtcgagaa agcggatcag ccggttgatg tcctcgcgat attgttcctt
     2941 gctgaccttc gccgcgcacg gcgcggtgca ttgcttgatc ggatacagca ggcaggggcg
     3001 gaagaaccgg cgcgaatcat cgctgtcgct gatctccagg tggcaggtgc ggaacttgaa
     3061 cgccttctgc agtagcgtca ccgcttcctt caactgatag acgctggtga acgggccgta
     3121 cagttttgta ttcggcagcg gctcgcgcgt gacatatacg ccggggaact cgtcccgcgt
     3181 cgtgatcatc agatacggga acgtcttgtc gtccagcagc cgcgcgttgt agcgtggctg
     3241 gatgtccttg atcagccggt tctccgccag cagggcttcg acttcactgt cggtctggat
     3301 cgtttcgaaa tcgacgacgt ggtcgagcag cccgaccttc ttgtgctcca gcttggtcga
     3361 tggctgaaag taactgccca cgcgatcgcg caggctggcg ctcttgccga cgtagatgac
     3421 gcgccctttg tcatccttca tcaggtagac gccgggcgct ttcggcaggc ctttggcttt
     3481 ctcggcgagt ttcagaagtc ggtcgtccat gacgttcggc cccggacggg cgcggtccca
     3541 tattgttggc acgtgccggt cattttgcca cgcgtttgcc tgtgattcat ctcaatcgta
     3601 tctcagggca acgaaaggga gattgaccat gtttcgcaaa ttgatcgtta ccgggattat
     3661 tacaaccgtc gtcgccgtgc tcgccacgcc ggccagcgcc gcgggtcacg tgcggcacta
     3721 cacgccgtcg cgcaccgcga cggttcacat gtcgcacagc aacggcgtcg ccaagcatca
     3781 cgtggcgaag ctgaccaaaa gcaagaaaag caagggcaag tcgcacaaag gcggcggaca
     3841 taagcataag tgattcgacg ccagcctttg tcgattgagc agagcacgag cccccgacgc
     3901 cagggcatga gtctcagacc cggcgtcggg gcgcgcgtcc gggcggtttc tggtccgcag
     3961 cgcgacaaac tcatcccgct ttcccgtgtc ggtctctaca atggtcccct ttgaccatcc
     4021 gacacggagc ggctcatgcg cgatcaactg ggttttctca tcctgatcgc ctgcctcatc
     4081 gccaccaccg gctgcctctt cacggccgac agccagaaga cgaccagcgg caacagcgtg
     4141 gccgaaggca ccttctcgca aatcacgctg ggcaagacca ccgttggctg ggtgaaggcc
     4201 acgctcggcg agccgacccg ccggtcgcag gcggatgacc acgaagtctg ggaatacagc
     4261 tacaccgaac ggaccgacag cagcgggtcg gtcttcctga tcttcggcgg ccacaactcc
     4321 acgcagacga cgcacaaggc gtacgtcgaa ttcaaagacg gcgtcgtcgt caacaaatgg
     4381 cgggcgtgag
//
LOCUS       DATIMH010000181         8740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5239194, whole genome shotgun sequence.
ACCESSION   DATIMH010000181 DATIMH010000000
VERSION     DATIMH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5239194"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2272)
                     /locus_tag="VLI90_03025"
     CDS             complement(<1..2272)
                     /locus_tag="VLI90_03025"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV13203.1"
                     /translation="MSPRQMIKRAVEHHQSGQLDEAERLYRAALDESPNDADAWHLLG
                     VLTGQRGNKEGARQLVARAIEIDPSADLYHVNLGTLNGACGHSELAVANFRRAIELNP
                     NVPAVVHAELAQALSALSKIEEAIHALQWAVRREPRAEWLVALAELLQRAGRQQEALD
                     RLKQAIQARPDLAEAHGALGLAMEREGRLAEAEACYHKALELNPDLVQALNNLGHVLN
                     GQRRWDEAIKVLERAVELHPNLAQAYNNLGDAFRGQNRSDAAIACYRQALQLNPNLQG
                     AWDSLGSILLSRRELPAAADAFRKSTALRPTSASLVDLARAAAGVDQYDEAIDALNKA
                     AELSPSASDPHRVLGVALRWCGRLDEAIDEFKRAMYLDPRDHAAHSGLLYAMLFHDRA
                     YTPEQIFQEHVEWGRRHASLTPSPAAPGEGGGEGDLERRAPLEKQNHPNPLPAYPAGP
                     EQAPPDKHHSPDRRLRIGYISPNFRNTAVLMFVLPILRNHDAWEVEVFCYSDAHAPDE
                     WTAQVRERADEWRDTSKMTDEQLARQIREDRIDIAVELSGHIGDGRLLALACKPAPIQ
                     ISYIGYQATTGVSAIDYFLTDGWADPPGQTERFFVERLIRLPESFFCYAPPPEAPAVG
                     PLPAAAAGFVTLGCQNNLAKVTPRTIELWSTILHAVPDARFMLLTPGSREVDERVLNA
                     FESHGIVRERIELVRRATPVEYLARYNRIDIALDPVPFNGHTTTCDAAWMGCPTVTLS
                     GQIYAHRYGGSVLRNVG"
     gene            2399..2614
                     /locus_tag="VLI90_03030"
     CDS             2399..2614
                     /locus_tag="VLI90_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13204.1"
                     /translation="MSLPAIEGVVENGAIRLQKGVQLPERTRVFIIVAEPDARPSARI
                     WTPRLAIPGQAAEFRKQVLKAPTDAKM"
     gene            2655..2999
                     /locus_tag="VLI90_03035"
     CDS             2655..2999
                     /locus_tag="VLI90_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13205.1"
                     /translation="MLRSLAGGSPLPNVLLLIDTLADVTLLPASAVKQLGIAPIAGAA
                     YDLIGFDGSTSSATAVELDMIFLGRAYRGRYLLIDDEHGVLGREVLANVAMLFDGPGQ
                     EWTEQRTGTKPS"
     gene            3032..4798
                     /locus_tag="VLI90_03040"
     CDS             3032..4798
                     /locus_tag="VLI90_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13206.1"
                     /translation="MWRFLGYRGNRFVLVAIPLLIGLAIVPPLVAHARRRDPTLLPRP
                     PISVQFDKVRPRMPDRIVSFHRGRADKQLIRLASQLGFNGVQFQIEGSTVNGVKDFAE
                     RDRREGLIDFCHSLGMTVTVWVHEMSDLPPPWMPERIGQVNVDNAALWKLLDDRYEWM
                     LRDEIPNVDGLCLTVVETQVRATDTAVMLKLVDLLQGKCRKYNKSIMVRTFVWYPDEF
                     ANVMAAVNQLPKDMVIMSKCVPQDWNLRGTDAAEIGHVGGRPQIIEFDVAGEYYRRNF
                     VANCMPAMLKRQFDYAVKNGASGICVRVDREDDNVLEQPNEVNLWTLGMLADGASDSL
                     DEIWHAWATNRFGSAAADGVIKALKPTGDVVAEMLSIGNFSFGDTRKFPYLGDEDVYG
                     QLHQNWWWDAKYDVEHVRAETGDAAFIADVQAGKAHAMQLANQSILDLIEVKDHLAPA
                     DYDILGTRLLSNRVQLSFRAPMALAVLHYRQMINAATPADHDAADQAMQQDLAAVRAA
                     ATPVYGKPRRIEWLDRTWYVGVPDDYDRNAIFGWAYQMDELRQGIRPRPITPRQLAIA
                     KAQLEGPGTKVPPNAGRERPPQ"
     gene            4795..6366
                     /locus_tag="VLI90_03045"
     CDS             4795..6366
                     /locus_tag="VLI90_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HSV13207.1"
                     /translation="MTPQQMFDNAVNEQRAGRFANAIAIYRQLQAANPNAPELHFQLG
                     TALQAMNKVSDSIAAYRAAIALRPDYAQAINALGTALRERALLDEAADAFRKAIALAP
                     EQATPHYNLAVVLLRQGRTAESIAAFERAIQLQPDLIEAVGTLAEVYRVQGDYDAAIA
                     QCQRALALRPEAPPALTIWGSVLMEMERYDEAMAMYDRALRANPDYAEAHWCRACLQL
                     MRGQLRQGWREYEWRVRVRDLRVPTLVSKLPRWDGSPLNGKRVLINVEQGMGDTINFV
                     RYAKKIANERGGRVVLDCQAPLVRLMQTVEGVEEVVERGQQLPPFDAHFPLMGLPALF
                     ETALQTIPAEVPYVSAPAEETARWRQRFDPADRRLRVGLAWSGSPSHANDRNRSMALA
                     QLAPLAAVADVRFYSLQKGPAADQVKSAPAGFDLVDWMAELNDFADTAALMMNLDLVI
                     CVDTSVAHLAGALAKDVWVLLPKGPDYRWLLDRLDSPWYPTMRLFRQKHRGDWADVVR
                     RVGEALGRRASANAE"
     gene            complement(6721..7119)
                     /locus_tag="VLI90_03050"
     CDS             complement(6721..7119)
                     /locus_tag="VLI90_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSV13208.1"
                     /translation="MPRRERDPKSTTGRFAYDGLERVIHERARLSILSSLAAHPEGLL
                     FTELRTLCALTDGNLSRQLTQLQDGRFVETWKGTRNNRPQTLVKLTESGRKRFLEYIG
                     VLEGLVADALSSTRTSTADASAARARLSAT"
     gene            complement(7106..7720)
                     /locus_tag="VLI90_03055"
     CDS             complement(7106..7720)
                     /locus_tag="VLI90_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13209.1"
                     /translation="MELNEALSKIAEIHLQMARTRLFRGYRSNTTLFSAVVAIATAAA
                     QALLVPDPAHHEMAYLSLWCTAAVVCLVVVGTGVVLRYWRTDSPVERELTLVAIQQFI
                     PCLVVGGLLTYIFARFAWGSVWLLPGMWTILFGMGILASRPLLPREIKWIGAFYLLCG
                     LMSIVWARVGEPFSPWAMGIPFGVGQAAAAAVLYLKLERTDAAA"
     gene            8049..>8740
                     /locus_tag="VLI90_03060"
     CDS             8049..>8740
                     /locus_tag="VLI90_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HSV13210.1"
                     /translation="MPHRGQQRGRSTRLKISRRRPALISAAIEALECRRLFVNSAWAF
                     PGADGHMLYQPQPLGDHVEDFGMAGYMGGTTPMPTAAVKATVSPIAGDNTANIQNAIN
                     AVAALPQDSTGMRGAVLLNPGTYPIDGILNINASGVVLRGSGAGQTFIYGTTNTDKTE
                     RTLINVNGSSTQTLSNTKNITDKYVPVGAISFTVNDASGFSVGDNVIVHRPSTQQWID
                     DLGMNLLTFPWTP"
BASE COUNT         1611 a         2706 c         2919 g         1504 t
ORIGIN      
        1 gccccacgtt ccgcagcacg ctgccgccgt agcggtgggc gtagatttgg ccagacaacg
       61 tcaccgttgg acaacccatc cacgcggcat cgcacgttgt cgtgtgcccg ttgaacggca
      121 ccggatcgag cgcgatgtcg atgcggttgt agcgcgccag atactccacc ggcgttgccc
      181 gtcggaccaa ctcgatccgc tcgcgcacga tgccgtgtga ctcgaaggcg tttagcacgc
      241 gctcgtccac ctcgcggctc ccgggcgtta gcagcatgaa ccgcgcatcc ggaaccgcgt
      301 gcaggatcgt gctccacaac tcgatcgtcc gcggcgtgac cttggccagg ttgttctggc
      361 aacccaacgt gacgaacccc gccgccgccg cgggcagcgg gccgaccgcc ggcgcctccg
      421 gtggcggggc gtaacagaaa aaactttccg gcaggcgaat caggcgctcg acgaagaatc
      481 gctcggtttg gccgggtgga tcggcccagc catcggtcag aaaataatcg atcgcggaaa
      541 cgccggtcgt cgcttggtag ccgatgtacg agatttggat cggcgccggc ttgcacgcca
      601 gcgcgagcaa ccggccgtcg ccgatatggc cgctcagctc gacagcgatg tcgatgcgat
      661 cctcgcggat ctgccgggcc agctgctcgt cggtcatctt gctcgtgtcg cgccactcgt
      721 cggcccgctc gcgcacttgc gcggtccact catcgggcgc gtgggcatcg gaatagcaga
      781 acacctccac ttcccacgcg tcatgattcc gcaggatcgg cagcacgaac atcagcaccg
      841 ccgtgttgcg gaagttcggc gagatgtacc cgatgcgcag ccgccgatcc ggcgagtgat
      901 gcttgtcggg cggcgcctgc tcgggtcccg ccgggtacgc cgggagaggg ttagggtgat
      961 tttgcttttc taaaggcgct cgacgttcca aatcaccctc acccccgccc tctcccggcg
     1021 cagcgggcga gggggtaaga ctggcgtgcc gccggcccca ttccacatgc tcctgaaaaa
     1081 tctgctccgg cgtgtatgcg cggtcgtgaa acagcatcgc gtaaagcaac ccgctatgag
     1141 cggcgtgatc gcgcgggtcg agatacatcg cgcgcttgaa ctcgtcgatc gcctcgtcca
     1201 atcgcccgca ccatcgcagt gcaacaccca gcacgcgatg tggatcagag gcgctcggcg
     1261 acaactccgc cgccttgttc agcgcgtcga tcgcttcgtc gtattgatcg acgcccgccg
     1321 ccgcgcgggc gagatcgacc aggctcgccg acgtcggccg aagcgctgtg cttttgcgaa
     1381 acgcatccgc ggcggccggc agttcgcggc gcgacagcag gatcgatccg aggctgtccc
     1441 acgccccctg cagattcgga ttcagctgca gcgcctgccg gtaacacgcg atggcggcgt
     1501 cactgcgatt ctggccgcgg aaggcatcgc ccagattgtt atatgcctgc gccagattcg
     1561 gatgcagctc gaccgcgcgc tcgagcacct tgatcgcctc gtcccaccgc cgctggccgt
     1621 tcagcacgtg gcccaggttg ttcagcgcct gcaccagatc cggattcagc tccagcgctt
     1681 tgtggtaaca cgcctccgcc tcggccagcc ggccttcgcg ctccatcgcc aaacccagcg
     1741 caccatgcgc ctcggccaga tccggacgag cctgaatcgc ctgcttcaat cgatccagcg
     1801 cctcctgctg ccggccggcg cgctgcagca attccgccag cgcgacaagc cattccgccc
     1861 gcggctcgcg ccgcaccgcc cactgcagcg cgtggatcgc ctcctcaatc ttgctcagcg
     1921 ccgacaatgc ttgcgccagc tccgcatgca ccaccgccgg cacgttcgga ttcagctcga
     1981 tcgcgcgccg aaaattcgcg accgccaact cgctatggcc acacgcgccg ttcagcgtgc
     2041 ccaggttgac gtgatacagg tcggcggatg gatcgatctc gatcgcccgc gcgaccagct
     2101 gccgagcgcc ttccttgttc ccgcgctgcc cggtcagcac gcccagcaga tgccacgcat
     2161 cggcatcgtt cggcgactca tccaacgccg cacggtacag ccgctccgcc tcgtccagtt
     2221 gtccggattg gtggtgctcg acggcgcgtt tgatcatctg acgtggggac attgctgcaa
     2281 cacctcaaag cgccaaaggc atatgcgacg ctcggcgggt actgtcccgc gtcgatgctg
     2341 cgcaggcaat agcacgctcg gccctgtcgc agggtataat gccaggcaga ggtgctgcat
     2401 gtcgcttcct gcaatcgagg gtgtggtcga gaacggcgcg attcggctgc aaaagggcgt
     2461 ccaactgcct gaacgcaccc gtgtgttcat aatcgtcgcg gagccggatg cgcgtccatc
     2521 agcccgaatt tggacaccgc gcctggcgat tcctggacag gccgcggaat tccgcaagca
     2581 agtcttaaag gcacccacag atgccaaaat gtgacacctc cagatacgac ccacctgccc
     2641 cggtggcgga ggtcatgctg cgcagcctcg caggtggttc accgctgccg aacgttctgc
     2701 ttctcatcga cacccttgct gatgtgacgc tgcttccagc gtcagccgtg aagcaactgg
     2761 gcatcgcgcc gattgccggc gcggcttacg atctcatcgg ctttgacggg agcaccagca
     2821 gcgcaacggc cgtggagctt gacatgatct tcctcggccg tgcttatcgc gggcgctatc
     2881 tgctgatcga cgacgagcat ggcgtgctcg gtcgagaagt gctggcaaat gttgcgatgc
     2941 tgttcgatgg ccccggtcag gaatggacag agcagcgaac gggaacaaag ccgtcgtgag
     3001 tcgggcggga tcaacattgg agaacatgtt catgtggcgg tttctcggat atcgaggaaa
     3061 caggttcgtt ctcgtcgcga ttccgctgct catcgggctg gcaatcgtgc cgccgctggt
     3121 cgcccacgcc cgccggcgtg acccgacgct gttgccgcgg ccgccgatca gcgtccagtt
     3181 cgacaaggtt cgcccgcgga tgccggatcg gatcgtttcg tttcatcgcg ggcgagccga
     3241 caagcagctg atccgcctcg cgtcgcagct cggtttcaac ggcgtgcagt ttcagatcga
     3301 aggatccacc gtcaacggcg tgaaggattt cgccgagcgc gatcggcgcg aaggcttgat
     3361 tgacttctgc cacagcctgg gcatgaccgt gacggtgtgg gtgcacgaga tgagcgacct
     3421 gccgccgccg tggatgccgg agcgaatcgg gcaagtcaac gtcgataacg cggcgttgtg
     3481 gaagctgctg gacgatcgat atgagtggat gctgcgcgat gagattccca acgtcgatgg
     3541 gctgtgtttg accgtcgtcg aaacgcaggt gcgggcgacc gataccgcgg tcatgctgaa
     3601 gctggtcgat ctgctgcagg gcaaatgtcg taaatacaac aaatcgatca tggtgcggac
     3661 gttcgtatgg tacccggacg aattcgccaa cgtgatggcc gcggtgaacc agctgcccaa
     3721 ggacatggtc atcatgagca agtgcgtgcc gcaggattgg aacctgcgcg gcacggacgc
     3781 ggcggagatc gggcacgtgg gtgggcggcc gcaaatcatc gagttcgacg tggccggtga
     3841 atattaccgc cggaacttcg tcgccaactg catgccggcg atgctgaagc ggcagttcga
     3901 ttacgcggtg aagaacgggg catccggcat ctgcgtgcgg gtcgatcgcg aagacgacaa
     3961 cgtgctggag cagccgaacg aagtgaacct gtggacgctg gggatgctgg ccgacggggc
     4021 gagcgacagc ctcgacgaga tctggcacgc ctgggcaacg aacagattcg gctcggccgc
     4081 cgccgatggg gtgatcaagg cgctcaagcc caccggcgac gttgtcgcgg agatgctgag
     4141 catcggcaac ttcagttttg gcgacacgcg gaagtttccg tatctgggtg acgaagacgt
     4201 gtacggccag ctgcaccaga attggtggtg ggatgcgaag tacgatgttg agcacgtgcg
     4261 ggcggagacg ggcgacgccg cgttcatcgc ggacgtgcaa gcgggcaagg cgcacgcgat
     4321 gcagctggcg aatcagtcga tcctcgattt gatcgaggtg aaggatcatc tcgcgccggc
     4381 cgactacgac atcctcggca ctcggttgct gagcaaccgc gtgcagctgt cgtttcgcgc
     4441 gccgatggcg ctggcggtgc tgcactatcg ccagatgatc aacgcggcga cgccggccga
     4501 tcacgatgcg gccgaccagg cgatgcagca ggatctcgct gccgtgcgtg ccgcggcaac
     4561 gccggtgtac ggcaaacccc ggcgaatcga atggctcgat cgcacgtggt acgtcggggt
     4621 gcccgatgac tacgatcgca acgcgatttt cgggtgggcg tatcaaatgg acgaactgcg
     4681 gcagggcatc cgcccgcggc cgatcacacc gcggcaattg gcgatcgcca aggcgcagct
     4741 ggaaggtccc ggaaccaaag tcccaccgaa cgccggtcgc gagcggccac cgcaatgacg
     4801 ccacagcaga tgttcgacaa cgcggtgaac gagcagcgcg ccgggcgctt cgccaacgcc
     4861 atcgcgatct accgccagct tcaggcggca aatcccaacg cgccggaatt gcacttccag
     4921 ctgggcacgg cgcttcaggc gatgaacaag gtgagcgatt cgatcgcggc gtatcgggcg
     4981 gcgatcgcgc tgcggccgga ttatgcgcag gcaatcaacg cgctgggtac ggcactgcgc
     5041 gagcgagcgc tgcttgacga ggcggccgac gcatttcgca aggcaatcgc gctggcgccg
     5101 gagcaagcga cgccgcatta caacctggcg gtcgtgctgc tgcggcaggg ccggacggcg
     5161 gagtcgatcg cggcgttcga gcgcgcgatc caattgcaac ccgatctgat cgaagcagtg
     5221 ggcacgctgg ccgaggtcta ccgcgtgcag ggcgattacg acgcggccat cgcgcagtgc
     5281 cagcgggcac tggcgctgcg cccggaggcg ccgccggcgc tgacgatctg gggttcggtg
     5341 ctgatggaga tggagcggta tgacgaggcg atggcgatgt acgatcgcgc gctgcgggcc
     5401 aacccggatt atgccgaggc gcactggtgc cgagcgtgcc ttcagctgat gcgcggccag
     5461 ctgcgccaag gctggcgcga atacgaatgg cgcgtgcgtg tgcgcgacct gcgcgtgccc
     5521 acgctggtgt cgaagctgcc gcggtgggac gggtcgccgc tgaacggcaa gcgcgtgttg
     5581 atcaacgtcg agcaggggat gggcgatacg atcaacttcg tgcgctacgc gaagaagatc
     5641 gcgaacgaac gcggcgggcg cgtcgtgctg gattgccaag cgccgctggt gcgattgatg
     5701 caaacggtgg agggcgtcga ggaggttgtc gagcgcgggc agcagttgcc gcccttcgat
     5761 gcgcattttc cgttgatggg attgccggcg ttgttcgaga cggcgctaca gaccattccg
     5821 gcggaggtgc cgtacgtttc ggcgccggcg gaagagacgg cgcgttggcg gcagcggttt
     5881 gatccggcgg accgccggtt gcgcgtgggg ctggcgtgga gcggcagccc gtcgcacgcg
     5941 aacgatcgca atcgatcgat ggcgctggcg cagctggccc cgttggcggc ggtggcggac
     6001 gtgcggtttt atagtcttca aaagggtccg gcggcggatc aggtgaagag cgcgccggcc
     6061 ggatttgatc tggtggattg gatggcggag ctgaatgact tcgccgatac cgccgcgcta
     6121 atgatgaacc tggacttggt gatctgcgtg gacacatccg tcgcgcacct ggcgggggcg
     6181 ctggcgaagg acgtgtgggt gctgctgccg aaggggccgg attatcggtg gctgctcgat
     6241 cgcctggaca gcccgtggta tccgacgatg cgcttgttcc ggcagaagca ccgcggcgat
     6301 tgggcggatg tcgtgcggcg ggtgggggag gcgttggggc ggcgcgcgag tgcgaatgcg
     6361 gaatgatgca gaatgcagaa tgcagaatgc agaatgatga cggaacggga gcctaggtcg
     6421 tccggagcgc agcacgtact cgtgcgcggc tcctgttttg gatgagagtt ggatcagcgt
     6481 cgcccaccgc gggggcggac cgttgattgc ggtcgtgaag cgtggacggc ccgtcgccac
     6541 ggtgacgaga cgggagaatg ctgcgtcgtc gcgctatcat gatcgttgcc gtcagcttcg
     6601 atcttgcgcg atcgctggta gggctggtcg cagatggcgg cggctagctt tatggcgtaa
     6661 agaatccggg cgaagaaggg cggcattttt cagctccaat tcctgttgat cgtgagttag
     6721 ttaagtggcg gacaggcgcg cgcgggcagc gctggcgtcg gcggtggatg tcctggtgct
     6781 cgacagcgca tcggcgacga ggccctcaag cacgccgatg tattccagga accgcttgcg
     6841 gccggactcg gtcagcttca ccagcgtttg cgggcggttg ttgcgcgtgc ccttccaggt
     6901 ctcgacgaac cgcccatcct gcagctgcgt cagctgccgg ctgaggttcc cgtcggtcag
     6961 cgcgcacagc gtcctcagct ccgtgaagag caggccttcg ggatgggccg ccagggacga
     7021 gaggatgctg agccgggcgc gctcgtggat cacgcgctcc aggccgtcgt aagcaaatcg
     7081 gccggtggtt gactttggat cgcgttcacg ccgcggcatc ggttcgctcc agcttcaagt
     7141 acaacacggc agcggcggcg gcctgaccga cgccgaaggg aatgcccatc gcccacggtg
     7201 agaacggctc acccacgcgg gcccagacga tcgacatcaa gccgcacagc agataaaacg
     7261 cgccgatcca tttgatctcg cgcggcagca gcggccggga tgcaaggatg cccatgccga
     7321 agaggatcgt ccacatgccc ggcagcaacc agacactgcc ccacgcgaag cgcgcgaaga
     7381 tgtaggtgag caacccgccg accaccaggc acgggatgaa ctgctgaatg gcgacgagcg
     7441 tgagttcgcg ttcgaccggc gaatcggttc gccaatagcg caggacgacg ccagtgccga
     7501 cgacgaccag gcagacgacc gccgccgtgc accagaggga gagatacgcc atctcgtgat
     7561 gcgccggatc ggggacgagc agcgcctgcg ccgccgcggt ggcgatggcc acgacggcgg
     7621 aaaagagcgt ggtgttggac cgatacccgc ggaacaggcg ggtgcgagcc atctgcaggt
     7681 ggatctcggc gatcttgctc agagcttcgt tgagttccat ggggagcgcc ccttcctctt
     7741 tatagcataa agtaatcaac ggctagcata agatcaagtc gaatttgcct tggcggcaat
     7801 cgcacggacg acagcatccg cgagccgagc gaaggttgca ggagcacgga tgggaagcgg
     7861 attatcagac gtgaagcagc tccgtcacag cccaggccgg tgctcaatgc aatcgattga
     7921 gaatagcaaa aacctttgat ttccggttct gatggcggta gagtgcgcgc tggtacacgt
     7981 acaggccgca cgactggtca ggcagacgga caatcagcgg ggttgataca actcgccgga
     8041 gggcttgcat gccgcatcgt ggtcagcagc ggggacggtc tacgcgtctg aaaatctcgc
     8101 gccgtcggcc tgcgctgatc agcgccgcca tcgaagcgct cgaatgccgg cggttgttcg
     8161 tcaacagcgc ctgggcgttt cccggcgctg acgggcacat gctctatcaa ccgcagccgc
     8221 tgggcgacca cgtcgaagat ttcggcatgg ccggttacat gggcggcacg acgccgatgc
     8281 cgacggcggc tgtgaaggcg acggtgtcgc ccatcgcggg cgataatacg gcgaatatcc
     8341 aaaatgcgat caacgcggtg gccgcgctgc cgcaagacag cacaggcatg cgcggcgcgg
     8401 tcctgctcaa cccaggcacc tatccgatcg atggaatatt gaacatcaac gcctccggtg
     8461 ttgtcctgcg cggctccggc gccgggcaga cgttcatcta cggcaccacc aacaccgaca
     8521 aaaccgaacg cacgctgatc aacgtcaacg gctccagcac ccagaccctc agcaacacca
     8581 agaacatcac cgacaaatac gtccctgtcg gcgcgatcag cttcacggtc aacgatgcgt
     8641 ccggcttcag tgtgggtgac aacgtcatcg ttcatcgccc cagcacgcag cagtggatcg
     8701 acgatctggg catgaacctg ctgacgttcc cgtggacgcc
//
LOCUS       DATIMH010000182         4563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1773542, whole genome shotgun sequence.
ACCESSION   DATIMH010000182 DATIMH010000000
VERSION     DATIMH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773542"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VLI90_03065"
     CDS             <1..1008
                     /locus_tag="VLI90_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13211.1"
                     /translation="HPIHEERPMRMTWLAGLLISLPLQSLLLAAAAPPATTEPTAALP
                     PTTQAAKANDFLRFVDKGSTGSRLETADVAYRNADGATVHLVAAVHIGERDYFEGLNE
                     SFKLRDAVLYEMVKPKDQPAPEHGAESHSTISQMQRMIKDALGLEFQLDVIDYTRPNF
                     VHADLDAETFERMQAERGESMTMLMVRQMLEQMANPPKDDANAPTSEMQLEDLIQVFT
                     RPDGERQVKLLLAKNLAQLEEGGMGLDAMDGTVILTERNKAAISTLERTLKEGKRDVA
                     VFYGAAHMPGLSKALVEMGFHPVAREWRRAWDLTIRADQPSAVENLLMNAAKGLDDLD
                     K"
     gene            1281..2258
                     /locus_tag="VLI90_03070"
     CDS             1281..2258
                     /locus_tag="VLI90_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV13212.1"
                     /translation="MNYRNVGATDLKVSEISFGCWTMGGLNWVNGTPNGWANVNEDEI
                     TAAIKVGLDAGVNHWDNADVYGNGKAERMLARCFERLGVTSADQIIATKVGHFPGTAE
                     HAYDPLHIRHQCEQSLKNLRRDYIDLYYLHHGNFGDGDQYLHPAAEAMQQLKREGKIR
                     HIGQSAYSDADFEKAVPVVKPAVLQSWAHAMDDGFIRPGSRVARLMDQHNLSFVAFSP
                     LAQGLLLDKFDPDRPPSFDEGDHRRGQKKFSREELLKLKPKLAKLKSRFGETADALAG
                     VALNYVLAQPRVCCVIPGFRNERQARSNASAAGKYLSEADVKYVQEALR"
     gene            complement(2665..3042)
                     /locus_tag="VLI90_03075"
     CDS             complement(2665..3042)
                     /locus_tag="VLI90_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSV13213.1"
                     /translation="MKPLFHPSIQDVTVEGILHALSDPVRVAIFAQMASSQCTHICSE
                     FLKIDQRAIPKSTLSQHFKSLREAGLIRSERRGVEMLNTSRCAEIEQRFPGLLNSISN
                     AYRVQAKSAGRQDTPSRRATRKA"
     gene            3171..3947
                     /locus_tag="VLI90_03080"
     CDS             3171..3947
                     /locus_tag="VLI90_03080"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSV13214.1"
                     /translation="MSNANSSSNSNLTGKVALVTGASKGIGAEIARQLAAAGAAVVVN
                     YASDKAGAERVVDAIKTGGGRALAVQADVSKPADVKRLFTESDKAFGGRLDVLVNNAG
                     VYSFAPLTEVTPEHFHKQFDLNVLGLILASKEAAERFGDAGGSIVNVSSMVSVKGFPT
                     TAVYSATKGAVDAVTRVLATELGSRKIRVNSVNPGVVITEGVQSAGLSGGDFEQGYIN
                     STALGRVGVPSDIAQVVLFLATPQSGWMTGETLFTTGGVR"
     gene            complement(3993..4439)
                     /gene="trxA"
                     /locus_tag="VLI90_03085"
     CDS             complement(3993..4439)
                     /gene="trxA"
                     /locus_tag="VLI90_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSV13215.1"
                     /translation="MSIVTCPKCGAKNRVDERRASEQTPKCGKCGSPLPVSSSSDGHP
                     VTITDDNFAREVLQAGATPVLVDAWATWCPPCRALAPTIDQLAAESGGRYRVGKLNVD
                     ENPRVAGQFRIESIPTMLIFKNGQLVDKLVGLMPKPAIESKLQQWR"
BASE COUNT          871 a         1338 c         1544 g          810 t
ORIGIN      
        1 catccaattc acgaggagcg tcctatgcgc atgacgtggc tcgccgggct gttgatctca
       61 ttgccgctgc aatcgctcct gctggctgcc gcggcgccgc cggcgacgac ggaaccaacc
      121 gccgcgttgc cgccgacgac acaggcggcg aaggcgaacg attttctgcg cttcgtcgat
      181 aagggatcga cgggttcgcg cctggagacg gccgatgtcg cctaccgcaa cgccgacggc
      241 gcgacggtgc acctcgtcgc ggcggtgcac atcggcgagc gcgattactt cgaaggcttg
      301 aacgaaagtt tcaagctgcg cgacgcggtg ctgtacgaga tggtcaagcc caaggaccaa
      361 ccggcgccgg agcacggggc ggagtcacac agcacgatca gccagatgca acggatgatc
      421 aaggatgcgc tggggctgga atttcagctg gacgtgatcg actacacgcg gccgaatttc
      481 gtgcacgccg acctggacgc cgagacgttc gagcgaatgc aagcggaacg tggcgaatcg
      541 atgacgatgc tgatggtgcg gcagatgtta gagcagatgg cgaacccgcc gaaggacgat
      601 gccaacgcgc cgacgtcgga gatgcagctg gaagatctca ttcaggtatt cacgcgcccg
      661 gatggcgagc ggcaggtgaa gctgctgctg gcgaagaacc tggcgcagct ggaagaaggc
      721 gggatgggcc tggacgcgat ggacggcacc gtcatcctca ccgagcgaaa caaggcggcc
      781 atcagcacgc tggagcgcac gttgaaggaa ggcaagcgcg acgtcgcggt tttttatggc
      841 gctgcccaca tgccgggctt gtcgaaggcg ctggtcgaga tgggctttca tcccgtcgcc
      901 cgcgaatggc ggcgggcttg ggatctgacg attcgggcgg atcagccgtc agcggtggag
      961 aacctgctca tgaacgcggc gaaggggctg gatgatttgg ataagtgagc ggcagctgtg
     1021 tcggcgcgct tgtctctttc tgacgggaag agccgggccg tgtcggtccg gaccaaccac
     1081 gccggggcga cacgccccgg ctctgccctt ggcaatcatc gggtggatgt aactaggctt
     1141 tgtggtccgg cgctgctctg gtcccctcgc ccggtactcc gggagagggg aaacggacca
     1201 gaatcgagcc atattccaag tcgtacccat cgggtcgcct gattgcctgt cccaaccatc
     1261 cgcatgtatc ctcccacgtc atgaactacc gcaacgtcgg cgcgaccgat cttaaggtga
     1321 gcgaaatcag cttcggctgc tggaccatgg gtggactcaa ctgggtcaac ggtacgccca
     1381 acgggtgggc gaacgtgaac gaagacgaga tcaccgcggc gatcaaggtg ggcctcgacg
     1441 ccggcgtgaa ccattgggac aacgccgacg tctatggcaa cggcaaggcc gagcggatgc
     1501 tggcgcgatg tttcgagcgg ctgggggtaa cgagcgccga tcagatcatc gcgacgaagg
     1561 tcgggcattt tccgggcacg gccgaacacg cgtatgaccc gctgcacatc cgccatcagt
     1621 gcgagcagtc gctgaaaaat ctgcggcggg attacatcga cctgtattac ctgcaccacg
     1681 gcaacttcgg tgacggcgat caataccttc accccgccgc cgaggcgatg cagcagttga
     1741 agcgcgaggg aaagatccgg cacatcgggc agagcgcgta cagcgatgca gatttcgaaa
     1801 aagcggtgcc ggtggtgaaa ccggcggtgc tgcagagttg ggcccacgcg atggacgatg
     1861 ggttcatccg cccggggtcg cgcgttgcgc ggctgatgga tcagcacaac ctgtcgttcg
     1921 tcgcattcag cccgctggcg caagggctgc tgctggacaa gttcgacccg gatcgaccgc
     1981 ccagctttga cgaaggcgac catcgccgcg ggcagaaaaa gttcagccgg gaagagttgc
     2041 tgaagctgaa gccgaagctg gcgaagttga agtcgcggtt tggggagacc gccgatgcgc
     2101 tggcgggcgt ggcgttgaac tacgtgctgg cccagccgcg ggtgtgctgc gtgattccag
     2161 gttttcgcaa cgagcgccag gcgcgcagca atgcgagcgc ggcggggaaa tatctgagcg
     2221 aggcggatgt gaagtacgtg caggaggcgc tgcggtgagg ggcgggtgcg cgtcatgctg
     2281 agccaagcac gtactcgtgc gccgtcgaag gatgtcaaaa agcgctatta aatttttccg
     2341 aatgcgccag gaatgatgaa tttcgcaagc accgtcgttg aagaaacgca tcccacgccg
     2401 ggttcggagc gtactccgac gacccggatg tcttgtgatc gagaagcatc ggggtcgtcg
     2461 gactacctcc gaacccggcg tggagggggg gtcgcgctta ctgagccgag aactgagtga
     2521 accgcgtccg tccgggcctg agccccgtat gtacatgcgg ggcttcgaac gtcgatgaat
     2581 gcctgcgaca tcaaatcgtt cgatcacgcc gcggctaggt ccttcgactc cggcgagtac
     2641 gcctgcgcac ggcatgacat ttttctaagc cttgcgtgtg gcgcgccttg agggggtatc
     2701 ctgccgcccc gcgctcttcg cctgcacacg gtacgcgttg ctgatgctgt tgagcagccc
     2761 gggaaagcgc tgttcgattt ccgcacacct tgaagtgtta agcatttcca cgccgcggcg
     2821 ttcgctgcgg atcaggcctg cttcgcgcag cgatttgaag tgctgcgaga gcgtggactt
     2881 ggggatcgcg cgctgatcga tcttcaggaa ttcagagcag atatgcgtgc attgggagct
     2941 cgccatctgg gcgaagatgg cgacgcgcac ggggtcggac agggcatgca ggatgccctc
     3001 gacggtgacg tcttggatcg atgggtgaaa caggggcttc atgacaagca gttatacgct
     3061 cgatttgact ctttgttcga tagttctatt attcggaaca tttgcaccat gcggaaggcg
     3121 ggcttccgat cggttgcagg tgcgtgcttc acgttccaag ggagttcgcc atgagcaacg
     3181 ccaatagcag cagcaacagc aatctgaccg gcaaggtcgc cctcgtcacg ggggcatcga
     3241 agggcatcgg cgcggagatc gcccggcagc tcgccgccgc gggggccgcg gttgtcgtca
     3301 actacgcatc ggacaaggcg ggggccgagc gcgtcgtcga cgcgatcaag accggcggcg
     3361 ggcgcgccct ggcggtgcag gccgacgtca gcaagcccgc cgacgtcaaa cgcctcttca
     3421 ccgagtccga caaagcgttc ggcggccggc tcgacgtgct ggtcaacaac gccggcgtct
     3481 attcgttcgc cccgctgacc gaagtcacgc ccgagcattt ccacaagcag ttcgacctga
     3541 acgtgctggg attgatcctg gcatcgaagg aggcggccga acgcttcggc gacgccgggg
     3601 gcagcatcgt caacgtctcc agcatggtat cggtcaaagg atttccaacg acggccgtgt
     3661 acagcgcgac caagggcgcg gtggacgcgg tgacgcgcgt gctggcgacg gagctgggct
     3721 cgcggaagat ccgcgtgaac tcggtgaatc ctggcgtggt gatcaccgaa ggcgtgcaat
     3781 ccgccggatt gtccggcggt gacttcgagc aggggtacat caactcaaca gccctgggtc
     3841 gcgtgggcgt gccgagcgac atcgcgcagg tcgtgctgtt cctggcgacg ccgcaaagcg
     3901 gctggatgac cggcgaaacc ctcttcacca ccggcggcgt gcgatgacga gccccgcgca
     3961 gtggcgttgt ttgcgcgacg agaaaatcgc gctcaacgcc actgctgcag cttcgactcg
     4021 atcgccggct tgggcatcag gccgaccagc ttgtccacca gttggccgtt cttgaagatc
     4081 agcatcgtcg ggatgctttc gatgcggaac tgccccgcga cccgcgggtt ctcatccacg
     4141 ttcagttttc cgacgcggta acgcccgccg gattcggcgg ccagttggtc gatcgtcggc
     4201 gccagcgccc ggcacggcgg gcaccaggtc gcccacgcgt ccaccagcac cggcgttgcc
     4261 ccggcttgca gcacttcgcg cgcgaagttg tcgtcggtga tcgtcacggg atgaccatca
     4321 ctgctgcttg aaaccggcag cgggctgccg cacttgccgc acttgggggt ttgctcgctg
     4381 gcgcgacgtt cgtccacgcg gttcttcgcg ccgcacttgg gacaggtgac gatggacatg
     4441 gcgtttgatt atcgccaagg gtcaagcgga acgcagcggg aaattcgaag caccaggtgc
     4501 gttgtcattc gaatcgcagc cgcggggctt tagcccgcgg accattgaga tagcgagcgg
     4561 tgc
//
LOCUS       DATIMH010000183         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2061469, whole genome shotgun sequence.
ACCESSION   DATIMH010000183 DATIMH010000000
VERSION     DATIMH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061469"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2025
                     /gene="mgtA"
                     /locus_tag="VLI90_03090"
     CDS             <1..>2025
                     /gene="mgtA"
                     /locus_tag="VLI90_03090"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HSV13216.1"
                     /translation="RLRALSRLDADAAIAQLSSAAPGLQPDEAARRLRNYGRNDVATI
                     ERQSLAGELLSRSVNPLNALLLSLAAVSYYLGEQRASAMIVTMVLLSVGLGFVQEHRS
                     NKTADALRRMVRTTATVRRGGAAGHLEVPMEEVVPGDIVLLSAGDMVPADLRLVSAKD
                     LFVNQSSLTGEAMPVEKFAHAVEDAADSAFDLQNMCFAGTSVLSGIGCGVVALTGQHT
                     TFGHVAGMLAARRAPTSFDKGIARFTWMMIGLIAVMVPLVFIINGLTKGDWFEALLFA
                     VAVAVGLTPEMLPMIVTVNLAKGAIEMARRRVIVKRLDSIQNFGAMDVLCTDKTGTLT
                     QDRIILKRHLDVHGDESTRVLKYAYLNSLHQSGLKNLLDVAVLSHVELEERIGMAGTY
                     DKIDEVPFDFERRRMSVVLSDENGKRLLICKGAVEEVFSVCTRYATDAGEGPLDATHF
                     ADAQRTTTQLNADGFRVVAVACKTLPQESRAYTAADEQDMTLAGYIAFLDPPKETCAP
                     ALAALSASGVCVKILTGDNDIVTRKVCHEVGLSVDRIVIGKELESMAPETLEALAESA
                     VVFAKVSPAQKAAIIEALRRRGHVVGFLGDGINDGPALHAADVGVSVDSAVDIAKESA
                     DIILLEKSLGVLNEGVLEGRKVFGNITKYIKMGASSNFGNMFSVLGASAFL"
BASE COUNT          378 a          596 c          667 g          384 t
ORIGIN      
        1 gcggcttcgc gcgctatcgc ggcttgatgc ggacgccgcc atcgcccagc tatcgagcgc
       61 tgcgcccggc ctccaaccgg atgaagcagc aagaagattg cggaattacg ggcgcaacga
      121 cgtggcgacg atcgagcggc agagcctcgc gggcgagttg ctgagccggt cggtcaaccc
      181 gctcaatgcg ctgcttttgt cgctggctgc cgtgtcgtac tacctcggcg aacagcgggc
      241 ttctgcgatg atcgtcacga tggtcttgct aagcgtcggg ctcggcttcg tccaggaaca
      301 tcgctccaac aagactgcgg acgcgctgcg gcgcatggtg cgcacgaccg ccaccgtgcg
      361 ccgcggcggc gctgctgggc accttgaagt gccaatggaa gaagtcgtgc cgggcgacat
      421 cgtgctgctt tccgctgggg atatggtccc cgccgacctc aggctcgtct cggcgaagga
      481 cctctttgtc aaccagtcgt cgctgaccgg cgaagccatg ccggtcgaga agttcgcgca
      541 tgccgtcgag gacgccgcgg acagcgcctt tgaccttcag aacatgtgtt ttgctgggac
      601 gtcggtgttg agcggtatcg gctgcggcgt ggtggcgctg acgggccagc acacgacgtt
      661 cggccacgtt gcgggaatgc tcgccgcacg gcgcgcgcca acgagctttg acaagggcat
      721 cgcgcggttc acttggatga tgatcggcct gattgcggtg atggtcccgc tcgtgttcat
      781 catcaacggc ctaaccaaag gcgactggtt cgaggccctg ctttttgcgg ttgcggttgc
      841 agtgggtctg acacccgaga tgctgcccat gatcgtgacg gtcaatctcg cgaagggcgc
      901 aatcgagatg gcaaggaggc gtgtcatcgt caagcgtctc gattcgatcc aaaacttcgg
      961 cgcgatggac gtgctgtgca ccgacaagac cggcaccctg acgcaggacc gcatcattct
     1021 caagcgtcat ctcgatgtgc acggcgacga gtccacccgc gtcctcaaat atgcgtactt
     1081 gaacagcctg catcagtcgg gcttgaagaa cctgctcgac gtcgccgtgc tctcgcatgt
     1141 ggagctggaa gagcgaatcg gcatggcagg cacgtatgac aagatcgatg aagtgccgtt
     1201 cgatttcgag cggcggcgca tgtcggtggt gctgtcggac gagaatggca agcgcctgct
     1261 catctgcaag ggcgcggtgg aggaagtgtt ctccgtctgc actcgctatg cgaccgatgc
     1321 aggcgaaggc ccgctcgatg cgactcactt cgccgatgcg cagcgaacga cgacgcaact
     1381 gaacgcggac ggctttcgcg tcgtggcggt tgcgtgcaag accttgccgc aagagtcgcg
     1441 ggcgtacacc gcggcagacg agcaggacat gacgcttgcc ggttatatcg cgtttttaga
     1501 cccgcccaag gaaacctgcg ccccggcgct tgcggcgctg tccgcaagcg gtgtttgcgt
     1561 gaaaatcctc accggcgaca acgatatcgt cacgcgcaag gtctgccacg aagtgggact
     1621 gtccgtcgat cgcatcgtga tcggcaagga actggagtcg atggcgcctg aaacgctgga
     1681 agcccttgcc gaaagcgcag ttgtgttcgc caaagtatcg cccgctcaaa aggcggcgat
     1741 catcgaagcc ttgcggcgac gcggccatgt cgtgggcttt ctgggcgacg gcatcaatga
     1801 tggcccggct ctgcacgcgg cggacgtggg cgtgtcggtg gatagcgccg tcgatatcgc
     1861 gaaggagtcc gccgatatca ttctgctgga aaagagcctc ggcgtgctca acgagggcgt
     1921 gctcgaaggc agaaaagtgt tcggcaacat cacgaagtac atcaagatgg gcgcgagttc
     1981 gaacttcggc aacatgttca gcgtgctcgg cgcgagcgcg ttcct
//
LOCUS       DATIMH010000184         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4082702, whole genome shotgun sequence.
ACCESSION   DATIMH010000184 DATIMH010000000
VERSION     DATIMH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4082702"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /gene="malQ"
                     /locus_tag="VLI90_03095"
     CDS             <1..994
                     /gene="malQ"
                     /locus_tag="VLI90_03095"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HSV13217.1"
                     /translation="DALRSAQRELADAINRHRFTQFLFFRQLDQLKRYANDHRVRLIG
                     DLPIFISGDSADVWSHPHLFQLDKHRRPTVVAGVPPDLFSKTGQRWGNPIYNWPAMQR
                     DNFAWWIARFRSALTQADLVRIDHFRGFAACWQIPADHPTAEHGRWVKAPGRQLFQTI
                     RQQIGQLPFIAEDLGTITPDVESLRDNLRLPGMRILHFAFNGDPTSPYLPHNYIRNAV
                     AYTGTHDNDTTVGWFNTLPPKHARFLHQYLGHPCTNRDIAWSLIRLCWSSVADCAIAP
                     LQDVLSLGSKARMNVPGTCNGNWRWRVREAQLKTETLDRLAEMTTVCGRAPHAG"
     gene            complement(1001..1393)
                     /locus_tag="VLI90_03100"
     CDS             complement(1001..1393)
                     /locus_tag="VLI90_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13218.1"
                     /translation="MRIPSSITPMKRRVRRKRRGAPAAAALTLVAASFDPVEETVLLT
                     FDRAIDVSGWVGSALTVNDPVSTVNLLGGTWIVEMMSAASVRVTLDTLGDASGSVVLL
                     VVDAGNGVVAAGDGAAWAGTGGVALPFG"
     gene            complement(1530..2213)
                     /locus_tag="VLI90_03105"
     CDS             complement(1530..2213)
                     /locus_tag="VLI90_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13219.1"
                     /translation="MGLIPDTKIGKIEFCESHVSPFTTNAVAIGTTAPAVTAWSALVT
                     AARAAYTAQQAAQLAAKNATTNLNVAVGAMMDATSSIIKQVRAKADLSGNGVYALASL
                     PVPATPTPVGPPGTPTNFTVSLDQTGVLKLKWKCANPAGGTIYQVWRRTTPTGEFAYL
                     GGSGTKDFVDATIPAGSSQVTYQIQGVRSTAVGEWAQFNVNFGVGSGGAFATIASVEE
                     TTPTAKLAA"
BASE COUNT          474 a         1031 c          592 g          375 t
ORIGIN      
        1 cgacgccctg cgatccgctc agcgcgaact cgccgacgca atcaaccgcc accgcttcac
       61 ccagttcctc ttcttccgcc aactcgatca gctcaagcgt tacgcgaacg accaccgcgt
      121 tcgattgatc ggcgacctcc ccatcttcat ctccggcgac tccgccgacg tctggtccca
      181 cccgcacctg ttccagctcg acaagcaccg ccgccccacc gtcgtcgcgg gggtcccgcc
      241 cgatctgttc tccaagaccg gccagcgctg gggtaacccc atctacaact ggcccgcaat
      301 gcagcgcgac aacttcgcct ggtggatcgc gcgcttccga tcagccctca cccaggccga
      361 cctcgtccgc atcgaccact tccgcggctt cgcagcctgc tggcaaatcc ccgccgatca
      421 tcccaccgcc gaacatggcc gatgggtcaa agcccccggc cgccaactct tccaaaccat
      481 ccgacaacaa atcggccaac tacccttcat cgccgaagac ctcggcacca tcacgcccga
      541 cgtcgaatcc ctgcgcgaca acctccgcct ccccggcatg cgcatcctcc acttcgcctt
      601 caacggcgat cccaccagcc catacctgcc ccacaattac atccgcaacg ccgtcgccta
      661 caccggcacc cacgacaacg acaccaccgt cggctggttc aacaccctgc cccccaaaca
      721 tgcccgcttc ctccaccaat acctcggcca cccctgcacc aaccgcgaca tcgcctggtc
      781 cctcatccgc ctctgctggt catccgtcgc cgactgcgcc atcgccccac tacaagatgt
      841 actcagcctc ggcagcaaag cccgcatgaa tgtccccggc acctgcaacg gcaactggcg
      901 ctggcgcgtg cgcgaagctc agctgaaaac cgaaacgctc gatcgattag cggagatgac
      961 aaccgtgtgc ggccgagccc cacacgctgg ctaaacccaa ttacccaaac ggcagcgcca
     1021 ccccacccgt ccccgcccac gccgccccgt cgcccgccgc caccaccccg ttccccgcat
     1081 ccaccaccag cagcaccacc gacccactcg catcgcccag cgtatccagc gtcacccgca
     1141 cgctcgccgc cgacatcatc tccacaatcc acgtcccgcc cagcaggttc accgtcgaca
     1201 ccggatcatt caccgtcagc gccgatccca cccaccccga aacatcaatc gcccgatcga
     1261 acgtcaacag caccgtctcc tcgaccggat caaacgaagc cgccaccaac gtcagcgccg
     1321 ccgccgccgg cgccccgcgc cgcttgcgcc gcacgcgccg cttcatcggc gtgatggagg
     1381 aaggaatacg catgacttgg ctcgcctcgt gtgcacgcgc gcttgccatc ctgagcgtac
     1441 tccgcgacgg atctcggccg gtcacagaac gcgcgcccgc gccccagatc cttcgccgaa
     1501 taccgctcag aatgacagcg gaaataaaac tacgccgcca acttcgccgt cggcgtcgtc
     1561 tcttcgaccg acgcgatcgt cgcgaatgcc ccgccgctgc ccacgccgaa gttgacgttg
     1621 aactgcgccc attcacccac cgcggtcgat cgcacgccct gaatctgata cgtcacctgc
     1681 gacgagcccg ccgggatggt ggcatccacg aaatccttcg tgcccgatcc gcccagatac
     1741 gcgaactcgc ccgtcggcgt cgtgcgccgc cacacctggt agatcgtccc accggcgggg
     1801 ttggcgcatt tccacttcag cttcagcacg cccgtctggt ccaacgacac tgtgaagttc
     1861 gtcggcgtgc ctggcgggcc gaccggcgtc ggcgtcgccg gcaccggcag tgacgccagc
     1921 gcatacacac cgttccccga caagtccgcc ttcgccctca cctgcttgat gatcgacgac
     1981 gtcgcatcca tcatcgcccc aaccgcgacg ttcaggttcg tcgtcgcgtt cttcgccgcc
     2041 aactgcgccg cctgctgcgc cgtgtaagca gcccgcgccg ccgtcaccaa tgcgctccac
     2101 gccgtaaccg ccggcgccgt tgtcccgatc gccactgcgt tcgtggtgaa cggcgacacg
     2161 tggctctcgc aaaactcgat cttcccaatc ttggtatccg gaataaggcc catgacacac
     2221 gctccaaaaa aaggtcacgc gtgcgacaag cccctccggc gatggcgcgc ggggccgaac
     2281 actcgacccc atcaaccggc gcgccgccga tgtttgtcac acaccgcatc catcggacga
     2341 gcgacgcccc ggctgaaggc gcaaatcccg atcgttggaa caccgtcccc cgcgcgccga
     2401 tgcccgcgcc gataaaaccg gccctgccgc gacgcgttgg caatcaagac ccatccagcg
     2461 acgatagaag at
//
LOCUS       DATIMH010000185        10809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1196635, whole genome shotgun sequence.
ACCESSION   DATIMH010000185 DATIMH010000000
VERSION     DATIMH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196635"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2354)
                     /locus_tag="VLI90_03110"
     CDS             complement(<1..2354)
                     /locus_tag="VLI90_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HSV13220.1"
                     /translation="MRLNVSQRCRLIAITCVLAGQLCMAQTTRPAQDDPAPSQRQLKL
                     YCVGYTHLDTQWRWSYPQVIAEFLRNTLHDNFSLFEKYPHYIFNFTGANRYMMFKEYF
                     PDDYQKLKQYIAAGRWFPGGSSVEEGDVNMPDGEAIVRQVLYGNEFFRREFGTQSNEF
                     MLPDCFGFQASLPSVLSHCGIVGFSTQKLTWGSAVGIPFNVGVWEGPDGGSVVAALNP
                     GDYNGSISEDLSHSKRWQSRIEAEGRASGFYSDYHYYGTGDRGGAPRTQSIEWLEKSV
                     AAGGPVKVISATSEQMFKDITPPQIAKLPRYKGDLLLTNHSTGELTSQSFIKRINRKN
                     EQLADAAERASVAADWLGAAPYPMEKINRAWILELGAHFHDTMAGTALPKAYEYSWNN
                     EFLAANQFAAALEDGAGAVARGMDTRTQQGTAIVVFNPLSIEREDVAEATVTLPSNET
                     DTVAVFDPAGHAVPSQVVERAGERVKLLFLAKLPSVGFATFEARSIAANASPASELSV
                     SENGLENKRFRVTLNKDGDIASVFDKANQREVLSAPARLAFLYEKPQQYPAWNMDYDD
                     RMRAPLDYVHGPCKVRVVENGPARVAIEVERQTQGSRFVQQIRLAAGQAGNRVEVSSV
                     IDWQTREASLEAVFPLTVGNPMATYESQSAAVQRGNNDPKKFEVPQQQWFDVTGSDGA
                     YGTAILNDCKYGSDKPDDHTVRLTLLYTPGVRGVYQDQATQDIGRHEMVYAIAPHAGS
                     WQQGGTPWEAQRLNQPLITFQSGAHDGALGRSFSLLRVNNDHVTV"
     gene            complement(2443..5004)
                     /gene="ptsP"
                     /locus_tag="VLI90_03115"
     CDS             complement(2443..5004)
                     /gene="ptsP"
                     /locus_tag="VLI90_03115"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HSV13221.1"
                     /translation="MAVDFCFKFPLPNGLHARPASHFQEITKRFRSKVELIHERTCYK
                     ANVRSVLSMVSADIKWEDPCRVQITGEDEQPAMEAVRSFLKNVLPHCDEALPEIAPAA
                     AGALSAAEAPLPRSLKSAGLKSYLRGTAVSGGVGWGDVVLMGCVALPPELEHETATNL
                     AVEQDKASSAIAQVSAAINAKIEAARHRQEIEVLKAHAAIVGDVALAESVAELIDGKR
                     RTAAQAIVDACRTFTDALRNAHSAYLRERILDIEDVCTQLLEKIVWPGAIRVTPRLTS
                     PSICVADQMTPGQFLALDREHLKGLVLSDGGTTSHTVILARSMNVPTLVRVAKAIATL
                     RPGQEAIVDGELGLLVTEIPEPVRRYYRKERDTLAQRRRQLDAFRTRGGKTTDGHTIE
                     VAANVASAQEVAIAMRGGAQGIGLFRTEMLFMDRAAPPGEAEQAEIYCAAVTAAGDRS
                     VIIRLIDIGGDKPAPYLNLPAEVNPFLGYRGARLYREFAELVKTQLRAILRGSVTGDV
                     KILVPMVCCLEEVRDVRQLLTEAAEELAKNGVKIDRLPPLGVMIEVPSVAFLMPELCR
                     EVDFFSIGSNDLIQYFLAADRANQKVSNLYTWSHPAFLRLLKSVVDQAKAHGKWIGLC
                     GEMGDAVAALPLLTGLGLDEISLSAPRLGDAKAAINASSFVQCRQLVDAAINCATRAE
                     VESLLQEASATSDANAPAFSADLIVQSDAVTKEEVIRQLTNTLHLAGRVPRRQALEEA
                     IWRREDTYSTGFGHGFAVPHCKCDCLTANSMAIARLATSVEWGSMDGKPVDVVILLAI
                     RPQDHAQQHMRIFARLSRLVMREEFRDHVRQTQDPVELAAYIERELDQRPQPANA"
     gene            complement(5043..5339)
                     /locus_tag="VLI90_03120"
     CDS             complement(5043..5339)
                     /locus_tag="VLI90_03120"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF014368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HSV13222.1"
                     /translation="MKIVAVTACPTGIAHTYMAAEQLEKTAKSLGHQIKVETQGAMGI
                     ENELSTSDIQQSDVVVFAVDIAVEQPERFFGRKLVQVSVQDAIKDPKGVIARLR"
     gene            complement(5366..6535)
                     /locus_tag="VLI90_03125"
     CDS             complement(5366..6535)
                     /locus_tag="VLI90_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HSV13223.1"
                     /translation="MRESLIKLRQYLLSGVSFAIPFIACGGIMIAAAIGFNAHYSPNA
                     PGDFSHAPPILKLIIDIGAAAFTLMPAVLAGYISYAMAGKPGLVPGMVGGWLATHLGV
                     FVVTATHAIVATAEPGTHEATAGFLGALLAGLLAGYIVNLLKKLRVPKYIKPIMPILI
                     IPIVSSTLIGFVMLKVAGIPIAQAMASLGNWLDAMGKGNGVLLAMILGTMIAFDMGGP
                     INKAAFFFGAGLVAEGHYTVMGCVAAAICTPPLGMGLATLINRKQWSAEEREAGIAGL
                     AMGMIGITEGAIPFAAADPLRVIPCICFGSATAAVIAMIGGVGDHAPHGGPIVLPVVD
                     HRLMFVLAIVVGTVVTAVAVNAVKMLTTRRAVVDTADSAPVTKPARASATAAVTA"
     gene            6731..7606
                     /locus_tag="VLI90_03130"
     CDS             6731..7606
                     /locus_tag="VLI90_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV13224.1"
                     /translation="MDDQPTKQIADALDSFLRRGGIQTVHFAGGESPPPQLAYLVNFP
                     RLSIILAGQDQMEIEEQGTTTTLTAKRGEAIFVPANCWNKPAWASRVKVLTFLFGKRQ
                     TGVSLVSQQKRSATPAAAMKAHFHRPLEGPAPGILDALMTLSTAERPSPTDRLLVEAL
                     LYSCLKLLKEPSGHTGGKADKTFQNICLYLQENFQFPLTRDSVADHFRLSPNHVSRLF
                     RSEGLMRFTDYLTWVRLDRAKYLLRNHNSTLGEVASSCGFSDIGYFCRVFKRRTKLTP
                     TAYRLQTESNAAEKA"
     gene            complement(7811..9526)
                     /locus_tag="VLI90_03135"
     CDS             complement(7811..9526)
                     /locus_tag="VLI90_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HSV13225.1"
                     /translation="MNYAASPIAISVFLLFVGFVLGISFYLGSKAKSARGYFAAHGQI
                     NWFVNGIAFAGDYLSAASFLGICGMIAFYGYDGFLYSIGYLAGWVVALFVIAEPIKRL
                     GKYTFADALNAKFNSRGVQVAAAVSTLLVSIFYLIPQMVGAGALVTPLLGLPHWAGVT
                     IVGTVVILIVVTAGMVSTTWVQFIKGSLLVVLCAMLTVAILARGFRVTMHTPPVPVAA
                     TQPSDVPAGGQVVSIGSSDGSQSTGPLGPIDFLSRINDVRVVQWTAKADPAHKGTTVY
                     TPKEVKVQTLMLPGGSPTFKGVRSAKWADKLDYLSLMLALFAGTASLPHILIRYYTVK
                     DAAAARKSTIVGIASIGFFYVLTLYLGLGAMTSGSIDPTNSNMAAPLLAKSFGELPFA
                     VISAVAFTTVLGTVSGLILAASGAVAHDLMENVFKHHPNENAKVGAAKWTAVVVGIIA
                     MVLGVLFEKLNVTFLVGWAFNVAASANLPSLVMLLFWRRTTRQGITAAITIGLISSLT
                     WLLLSAPAYQYVYNADPAKAIAPFTQPGLVTIPLGFIVLVVVSLATSGREVTSKARRS
                     ARAAA"
     gene            complement(9523..9774)
                     /locus_tag="VLI90_03140"
     CDS             complement(9523..9774)
                     /locus_tag="VLI90_03140"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HSV13226.1"
                     /translation="MAFNARLGVVLFLVYLLLYVGFIYLSAFQRERMAVEVIGRVNLA
                     VVYGFALIAAAFIMALLYMVLCRREPAVDANDTAAEGGR"
     gene            complement(10003..10563)
                     /locus_tag="VLI90_03145"
     CDS             complement(10003..10563)
                     /locus_tag="VLI90_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13227.1"
                     /translation="MAAFDGRLWGSLLVAALVASGLFSLSAVSIVRYHRKLGVIGKRL
                     SEAEPVEVLEVRASRVVDVQAPGSTGPALCFELADGQVLLLYGQWLLEYSLYRAPPPA
                     DDGNDAHFNGLDEPHAFPSDHFSIHRWRGDVRPFWIEVHGRYLDPVNAPVQLGRSRRI
                     REVELFPGSFGSLQADIDRAFADRTT"
BASE COUNT         2098 a         3696 c         3089 g         1926 t
ORIGIN      
        1 accgtcacgt gatcgttgtt cacgcgcagc agcgaaaacg atcggcccag cgcgccatcg
       61 tgggcaccgc tctgaaacgt gatcaacggc tgattcaacc gctgcgcttc ccacggcgtc
      121 ccaccctgct gccacgaccc cgcgtgcggc gcgatcgcat aaaccatctc gtgccgcccg
      181 atgtcctgcg tcgcctggtc ctgatacacg ccgcgcacgc ccggcgtgta tagcagcgtc
      241 agccggaccg tgtgatcgtc cggcttatct gagccatact tgcagtcgtt caagattgcc
      301 gtgccatacg cgccgtcgct gccggtaacg tcgaaccact gctgctgcgg cacctcgaac
      361 ttcttcggat cgttgttacc gcgctgcacg gcggccgact gcgattcgta cgtcgccatc
      421 ggattaccga ccgtcagcgg gaacaccgct tccaggctcg cctcgcgcgt ctgccaatcg
      481 atcacggacg aaacctcgac gcgattgccc gcctgccccg cggccaaccg aatctgctgc
      541 acaaaccgcg agccctgcgt ctgccgctcg acttcgatcg ccacgcgcgc cggcccattc
      601 tccaccaccc gcaccttgca cggcccatgc acgtaatcca gcggtgcccg catgcggtcg
      661 tcataatcca tgttccacgc ggggtactgc tgcggcttct cgtacaaaaa cgccaaccgt
      721 gccggcgcgc tcagcacctc gcgctggttc gctttgtcga agacggatgc gatatcgccg
      781 tccttattca gcgtcacgcg aaaccgcttg ttctccagac cattttcgct gacgctcaac
      841 tcagaagccg gcgaagcatt cgccgcaatc gatctcgctt caaatgtcgc gaagccgacg
      901 gagggcagct tcgccaggaa cagaagcttc acgcgctcgc cagcgcgctc gaccacctgc
      961 gaaggcaccg cgtgccccgc cgggtcgaag acggcgacag tatccgtctc attcgatggc
     1021 agcgtgaccg ttgcttcggc cacgtcttcg cgctcgatcg acagcggatt gaagacaacg
     1081 atcgccgtcc cctgctgcgt tcgcgtgtcc atgccgcgcg cgaccgcgcc cgcgccgtct
     1141 tccaatgcgg cggcgaactg attggccgcg agaaattcgt tgttccacga atactcgtac
     1201 gccttcggca acgccgtgcc ggccatcgtg tcgtgaaaat gcgcgcccag ctcaaggatc
     1261 cacgcgcggt tgatcttctc cattggatac ggcgccgccc ccagccaatc cgcggcgacc
     1321 gatgcgcgct cagcggcgtc ggccagctgc tcgttcttcc gattgatccg cttgatgaac
     1381 gactgcgacg tcagctcgcc cgtggagtgg ttcgtcagca gcagatcgcc tttatagcgc
     1441 ggcagcttcg cgatctgcgg cggcgtgatg tccttgaaca tctgctcgct cgttgccgag
     1501 atcaccttca ccggcccgcc ggcggcgacg ctcttttcca gccattcgat cgactgcgtc
     1561 cgcggcgccc cgccgcgatc gcccgtgccg taatagtgat aatcgctgta gaaaccagac
     1621 gcgcgaccct ccgcttcgat gcgcgactgc caccgcttgc tgtggctcaa gtcttcgctg
     1681 atcgatccgt tgtaatcgcc gggattgagt gccgcgacga cgctgccccc gtcggggcct
     1741 tcccacacgc cgacgttgaa cggaatgccg acggccgatc cccaggtcag cttctgcgtc
     1801 gaaaatccga cgatgccgca gtgcgacaac acgctcggca atgacgcttg aaacccgaaa
     1861 caatccggca acatgaattc gttgctctgc gtgccgaact cgcggcggaa gaattcgttt
     1921 ccgtacagga cctggcggac gatcgcctcg ccatcgggca tgttcacgtc gccttcttcc
     1981 accgacgaac cgcccgggaa ccaccgcccc gccgcgatgt actgcttcag cttctggtaa
     2041 tcatccggaa aatactcctt gaacatcatg tagcgattag cgccggtgaa attgaagatg
     2101 tagtgcggat acttttcgaa caggctgaag ttgtcgtgca gtgtgttgcg aaggaattcg
     2161 gcgatgacct gcggatagct ccagcgccac tgcgtatcga ggtgcgtgta accgacgcag
     2221 taaagcttca gctgccgctg cgacggcgca ggatcatcct gcgccggccg cgtggtctga
     2281 gccatgcaca gttgccccgc gagcacgcag gtgatggcga tcaatcgaca gcgttgcgaa
     2341 acgttcaatc gcatttgaga aatatccttc cggcccacgt gcgccgatgg cgtttgattc
     2401 tacagggata taagaccgcc tcggctgccg gcatccgcgc gcttatgcgt tggccggctg
     2461 cggtcgttgg tccagttcac gttcgatata cgccgccagc tccaccgggt cctgcgtctg
     2521 tcgaacgtgg tcccggaatt cttcgcgcat caccaaccga gagagccgcg cgaaaatccg
     2581 catgtgctgt tgtgcatgat cctgcgggcg gatggccagc aggatgacga cgtccaccgg
     2641 tttgccgtcc atcgagcccc actcgaccga cgtcgccagg cgggcgatcg ccatcgagtt
     2701 ggccgtcagg caatcacact tgcaatgtgg cacggcaaat ccatgaccga atccggtcga
     2761 atacgtgtcc tcgcgccgcc agatcgcttc ttccagcgcc tgccgccgcg gcacccgccc
     2821 ggccaagtgc agcgtgttgg tcagctggcg gattacttct tccttggtca ccgcgtccga
     2881 ctgcacaatc aaatccgctg aaaaagccgg tgcgtttgcg tcgctcgtcg ccgatgcttc
     2941 ctgcagcagc gattcgactt ccgcgcgcgt cgcacagttg atggcggcat cgaccagttg
     3001 ccggcactgc acgaaactcg atgcgttgat cgccgccttc gcgtcgccca accgcggcgc
     3061 cgataagctg atctcgtcca gccccagccc ggtcaacagc ggcagcgccg ccacggcatc
     3121 acccatctcg ccgcacaggc cgatccactt gccgtgcgct tttgcttgat cgaccacaga
     3181 cttcagcaac cgcaggaacg ccgggtgcga ccacgtgtac aggttcgaca ccttctggtt
     3241 ggcgcgatcg gcggccagga aatattgaat caagtcattg ctgccgatgc tgaagaaatc
     3301 gacctcgcgg caaagctccg gcattaaaaa agcgaccgac ggcacctcga tcatcacgcc
     3361 cagcggcggc aggcgatcga tcttcacgcc attcttcgcc agttcctcgg cggcttcggt
     3421 caacagctgc cgcacgtcgc gcacctcttc caggcagcac accatcggca ctaggatctt
     3481 cacatcaccg gtgacggagc cgcgcaagat cgcgcgcagc tgcgtcttca ccagctcggc
     3541 gaattcccga tagagccggg cgccgcgata acccagaaac ggattcactt ccgccggcag
     3601 gttcaggtat ggcgcgggct tgtcgccgcc aatgtcgatc agccgaatga tgaccgaccg
     3661 atcacccgcg gcggtgacgg cggcgcaata aatctccgcc tgctccgcct cgcccggtgg
     3721 cgcggcgcga tccatgaaca acatttcggt gcgaaacagg ccgatgccct gcgcgccgcc
     3781 gcgcatcgca atcgccacct cttgggcgct ggcgacgttg gcggccactt caatcgtatg
     3841 accgtcggtc gtcttcccgc cgcgcgtgcg aaatgcatcg agctgacgac ggcgctgcgc
     3901 cagcgtgtcg cgctccttgc ggtaataccg ccgcaccggc tcgggaatct ccgtcaccag
     3961 caggccgagt tcaccgtcaa cgatcgcttc ctgccccggt cgaagcgtcg cgatcgcttt
     4021 ggccacgcgg accagcgtcg gcacgttcat cgatcgcgcg agaatgaccg tgtgcgacgt
     4081 cgtcccgcca tcgctgagca ccaggccctt gagatgttcc cggtccagcg ccaaaaattg
     4141 tcccggcgtc atctgatcgg ccacgcagat cgacggcgac gtcaaccgcg gcgtcacccg
     4201 aatcgcgccc ggccagacga tcttctccag cagctgcgtg cagacgtctt cgatgtcgag
     4261 gatgcgctcg cgcagatagg cactgtgggc gttgcgcagc gcatcggtaa atgttcggca
     4321 ggcgtcgacg atcgcctgcg ctgccgtgcg acgcttgccg tcgatcaatt ccgccaccga
     4381 ctccgccagc gcgacatcac cgacgattgc cgcgtgcgct ttgagcacct cgatttcctg
     4441 ccggtgccgt gccgcttcga tcttcgcgtt gatggcagcg ctcacttgcg cgatcgccga
     4501 cgatgccttg tcctgctcca ccgccaagtt cgtggctgtt tcgtgttcca gttccggcgg
     4561 tagcgcgacg cagcccatca gaacgacgtc accccagcct accccaccgc tgacggccgt
     4621 gccgcgcaga tacgatttca agcccgcgct cttcagcgat cgcggcagcg gcgcctcagc
     4681 cgccgacaac gcgccagccg ccgccggggc aatctccggc agcgcttcat cgcagtgcgg
     4741 cagcacgttc ttcagaaaac ttcgcaccgc ctccatcgcg ggctgttcgt cttcgccggt
     4801 gatctgcacg cggcaggggt cttcccactt gatgtccgcg ctgaccatcg acagcacgct
     4861 gcgcacgttc gccttgtaac aggtgcgctc gtggatcaat tccaccttcg agcggaatcg
     4921 cttggtgatc tcctgaaaat ggctcgccgg ccgggcgtga agcccgttgg gcagcggaaa
     4981 tttgaaacaa aaatcgacgg ccatttcaca aaccttgcaa caaattacaa acatcaaaac
     5041 gcttaccgaa gccgcgcaat cacgcccttg ggatccttga tcgcatcctg caccgacacc
     5101 tgcaccagct tgcggccgaa gaaccgttcc ggttgttcca ccgcgatatc aaccgcgaac
     5161 acgaccacgt ccgattgctg gatgtcgctg gtgctcaatt cgttttcgat gcccatcgcg
     5221 ccttgcgtct caaccttgat ctgatggccc agcgacttgg ccgtcttttc cagctgctcc
     5281 gccgccatgt acgtgtgcgc gatgcccgtg ggacacgcgg tgactgctac gattttcata
     5341 aacactctct ctagctattc gtgtttcaag ccgtcacggc ggccgtcgcg ctggcgcgcg
     5401 ccggtttcgt cacgggcgcc gaatcggccg tgtcgacgac cgcgcgacgc gtggtcagca
     5461 tcttcaccgc attgacagcg accgccgtca caacggtgcc gacgacaatc gccaggacaa
     5521 acatcaaccg atgatccacc accggcagca cgatcggccc gccgtgcggc gcgtggtccc
     5581 ccacgccgcc gatcatggcg atgaccgccg ccgtcgccga accgaagcag atgcacggaa
     5641 tgacccgcag tggatcggcc gccgcgaagg gaatcgcccc ttcggtgatg ccgatcatgc
     5701 ccatcgccag cccggcaatg cccgcttcgc gctcttccgc cgaccactgc tttcgattga
     5761 tcaacgtcgc aagccccatc cccagcggcg gtgtgcagat ggccgcggcc acgcagccca
     5821 tcactgtgta atgtccctcg gccaccagcc ccgcgccgaa gaagaacgcc gccttgttga
     5881 tcggcccacc catgtcgaac gcgatcatcg tccccaggat catcgccagc agcacaccgt
     5941 tgcccttgcc catcgcatcg agccaattgc ccagcgacgc catggcctgc gcgatcggaa
     6001 tgccggcgac cttcagcatg acgaacccga tcagcgtcga tgagacgatc gggatgatga
     6061 ggatcggcat gatcggcttg atgtatttgg gaacccgcag tttcttcaaa aggttgacga
     6121 tgtacccggc gagcaacccc gccagcaacg cgccaagaaa ccccgccgtc gcttcgtgtg
     6181 tgcccggctc ggccgttgct acgatggcat gtgtagcagt cacaacgaac acacccaggt
     6241 gcgtggccag ccaaccgccg accatacccg ggaccaggcc gggctttccg gccatcgcat
     6301 acgagatgta cccggcgagc accgccggca tcagcgtgaa ggcggccgcg ccaatatcaa
     6361 tgatcaactt caagatcggc ggcgcgtgcg aaaaatcgcc cggcgcgttg ggtgaatagt
     6421 gtgcgttgaa cccgatcgcc gccgcgatca tgatcccgcc gcaggcgata aacggaatcg
     6481 cgaacgacac cccgctcagc aggtactggc gtaatttaat cagtgactct cgcatcgcgc
     6541 ttgctcctcg cccacgcaat tgccgcggcg cgggccagcc tcgtttggca accctcgccg
     6601 cactcctacc ttaaatgacg ccagcggggc taaaaagtgg aatgtcgtat cattcgttgg
     6661 ataatcgtac cattgcgatg gtacagtagg acaacaccac acgcgcgcgg gcagacgggc
     6721 ggaccgacag gtggacgacc agccaaccaa gcagatcgcc gatgcgctgg acagcttcct
     6781 gcgccgcggc ggcattcaaa ccgtccattt cgccggcggg gaatcaccgc cgccacagct
     6841 ggcgtatctc gtgaacttcc cgcgcctctc gatcatcctc gccgggcaag accagatgga
     6901 gatcgaagag caaggcacga ccaccacgct gaccgccaag cgcggcgaag caatcttcgt
     6961 ccccgccaac tgctggaaca aaccggcctg ggcctcgcgc gtgaaggtgc tgacgttcct
     7021 gttcggcaag cgacagaccg gcgtgagcct ggtcagccag cagaaacgca gcgcgacgcc
     7081 ggccgcggcg atgaaggcgc atttccatcg cccgctcgaa ggcccggcgc cgggcatcct
     7141 cgacgcgctg atgacgctga gcaccgccga acggccgtcg cccaccgatc ggctgctggt
     7201 cgaagcgctg ctctattcgt gcctgaaact tctcaaggaa cccagcggcc acaccggcgg
     7261 gaaggcggac aaaacgttcc agaacatctg cctttacctg caggaaaatt ttcaatttcc
     7321 cctgacgcgc gactcggtcg ccgatcactt ccgcctctcg cccaatcacg tctcgcgcct
     7381 gttccgcagc gaaggcttga tgcgcttcac ggactacctg acgtgggtcc ggcttgatcg
     7441 cgccaagtat ctgctgcgca atcacaacag cacgctcggc gaagtcgcca gcagctgcgg
     7501 gttcagcgac atcgggtact tctgccgcgt gttcaaacgc cgcacaaaat taacgcccac
     7561 tgcatatcga ctgcagaccg agagcaatgc ggcggagaag gcgtgatcgc tccgaacgtg
     7621 cagggttgaa gattgcgcca agctcgccaa gacgcaaaga cgccaaggaa gaaagagagc
     7681 gtcaattcgt agcacgggca tgtcaccggt gtcgaacggc gccaggcacg ggcgagacgc
     7741 ccgtgctaca tgttggcgcg agctggcgaa cggtgcgtgt gccgcgctca ttgttcattt
     7801 gtatcatttt ttaagccgca gctctggctg atcggcgcgc ctttgaagtg acttcgcgcc
     7861 cgctcgtcgc cagcgacacg accaccaata cgatgaaccc caatgggatc gtcaccaggc
     7921 ccggctgagt gaacggcgca atcgccttcg ccggatcggc gttgtacaca tactgatacg
     7981 ccggcgcgct cagcagcagc caggtgaggg agctgattaa cccgatcgtg atcgccgcgg
     8041 tgatgccctg ccgcgtcgtt cgccgccaga acagtagcat gaccagcgag ggcagattcg
     8101 ccgacgcggc cacgttgaac gcccagccga ccaggaacgt cacgttcagc ttctcgaaca
     8161 gcacgcccag caccatcgcg atgatgccga cgaccaccgc cgtccacttg gcggcgccca
     8221 ccttcgcgtt ctcgttcggg tgatgcttga aaacgttctc catcaggtcg tgcgccaccg
     8281 cgccgctggc ggccaggatc aaaccgctga ccgtgcccag cacggtcgtg aacgcgacgg
     8341 ccgagatgac cgcgaacggc agttcgccga acgacttggc cagcagcggc gcagccatgt
     8401 tgctgttggt cggatcgatc gatccgctgg tcatcgcgcc caaccccagg tacagcgtca
     8461 gcacgtagaa gaacccgatg ctggcgatgc cgacgatcgt gctcttgcgc gccgccgccg
     8521 cgtctttcac ggtgtagtag cggatgagga tgtgcggcag cgatgccgtg cccgcgaaaa
     8581 gcgccagcat cagcgaaaga taatccagct tgtccgccca cttcgccgag cgcacacctt
     8641 taaacgtcgg cgatccgccc ggcagcatca gcgtctgcac cttcacttcc ttcggcgtgt
     8701 aaacggttgt ccccttgtga gccgggtccg ccttcgccgt ccactggacg acgcgcacgt
     8761 cgttgatgcg gctcaaaaaa tcgatcggtc ccaatggccc cgtgctctgg ctcccatcgc
     8821 tcgatccgat gctcacgacc tgcccgcccg cgggcacgtc gctcggttgc gtcgccgcca
     8881 ccggcacggg cggcgtgtgc atcgtcacgc gaaagccgcg cgccaaaatc gcgaccgtca
     8941 gcatcgcgca cagcacgacc agcagcgagc ccttgatgaa ctgcacccac gtcgtgctca
     9001 ccatccccgc cgtcacgacg attagaatga cgaccgtgcc gacgatcgtc acgcccgccc
     9061 agtgcggtaa tcccagcaat ggcgtcacca gcgcgccagc tccgaccatc tgcgggatca
     9121 agtaaaagat gctgaccagc aacgtgctga ccgccgccgc cacttgcacc ccacggctgt
     9181 tgaacttcgc gttcagcgca tcggcgaagg tatatttgcc cagtcgtttg atcggctcgg
     9241 caatcacgaa cagcgcgacc acccacccgg cgagataacc gatcgagtag agaaacccat
     9301 cgtacccata gaacgcgatc atcccgcaga tgccgaggaa cgacgccgcc gacaggtaat
     9361 caccggcgaa ggcgatgccg ttgacgaacc agttgatctg accgtgagcc gcgaaataac
     9421 cgcgcgcgct cttggctttg gagccaagat aaaagctgat gcccagcacg aagccgacga
     9481 acagcaggaa gacgctgatc gcgatcggcg aagcggcgta attcatcgcc cgccctccgc
     9541 cgctgtgtca ttcgcgtcca ccgccggctc gcgccggcag agcaccatgt acagcagcgc
     9601 catgatgaat gccgccgcga tgagggcgaa gccgtagaca acggcgaggt tcacccggcc
     9661 gatcacttcc accgccatcc gctcgcgctg gaacgcgctg aggtaaatga acccgacgta
     9721 cagcagcagg taaacgagaa agagcacaac gccgaggcgc gcattaaacg ccacgaccgc
     9781 gtcccgcgca gcccggccgg tttcagatat aggcacgatg tgtggtctcc aatttcccta
     9841 gagccgcgcg atccccgtga agggccagtg tacttcgaaa gccgcacctc gactacaacg
     9901 gcgtatttat cgctaaacgc cacgacggtc cagatgcgca aaaacaacgc cacgtttaga
     9961 tttagcggcc gcgtcaatac aacattccgt ctgttgcggc tgctacgtcg tccgatcggc
    10021 gaacgcccgg tcgatgtccg cttgcagcga tccaaacgat ccgggaaaga gttccacttc
    10081 gcggatccgt cgtgatctcc cgagttgtac aggggcattc accgggtcca gatatcgccc
    10141 atgaacttcg atccagaacg gtcgcacgtc gccacgccaa cgatgtatcg agaagtggtc
    10201 ggacggaaac gcgtgcggct catctaggcc gttgaagtgc gcatcgttgc cgtcgtccgc
    10261 cggaggtggc gcgcggtaaa gcgaatactc gaggagccat tgtccgtaga gcagcaggac
    10321 ctgcccgtcc gcgagctcga agcatagcgc tggaccggtg ctgcccggcg cttgcacgtc
    10381 gaccacgcgc gatgcgcgaa cctccagcac ctcgaccggc tctgcttcgg aaaggcgttt
    10441 cccgattacg ccgagcttcc ggtgataacg gacgattgaa accgccgaca gcgaaaacag
    10501 gccgctcgcc accagcgccg ccaccaatag cgaaccccac agccgtccat cgaaagcagc
    10561 catcgcccgg gtggcgagga atgctacagc gaaaacgccg cagccgagga tgagaaacga
    10621 gcggaggagg tgcgatggca gcgacgccga agcagtgtcc ccggttccga tgtccgcttg
    10681 cagccctttt cgatatcgtg gcgtaagcat agtggcggca cgatctcgag agtcagcata
    10741 cccgcgctcg ctggcgcgaa gtgcgcgcga tcgtttagcc gctgcggcct cgcggcgcga
    10801 actttcaaa
//
LOCUS       DATIMH010000186         4972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_330516, whole genome shotgun sequence.
ACCESSION   DATIMH010000186 DATIMH010000000
VERSION     DATIMH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330516"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(537..2360)
                     /locus_tag="VLI90_03150"
     CDS             complement(537..2360)
                     /locus_tag="VLI90_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HSV13228.1"
                     /translation="MPTPQANLFQQALAHHQAGRLGPARAAYEQVLAAEPNHADALHM
                     LGVVNAQEGRPDAAVQLIHRALRIKPRYPEALTNLALALTALGRVTEAADALKKAAEL
                     QPNDPLVHFHQAETLLRVGRIEEARAAYARTVQLNPNFAAALVGLGVTLGELDRVDEA
                     TRALDRAIELAPNDAMAYQAKGIILHRHPAKMHEATDTLRRAVELAPQLAAAWNALGS
                     ALRAMGQLDEAARCFARASELRPDIALFATNLASITKAAGAAVDAQVAPLLAVLSRPD
                     ASELDRVGAGFAVGKLLDEAGRFDDAFGYFATANARVKQQAAAHGDRFDSDLLRRQID
                     QIIARFTPAFFQEHRDWGNPSQLPVFIVGMPRSGTTLVEQIAASHPAVVGAGELKDID
                     RISAGLGRDPAGWTRAAIAAAAAQHLERLQAIARDHVAARGGGDEGGAVQTQRVVDKM
                     MGNAIELGLIATMFPGARVIICRRDPRDNCLSCFFQHFASGNLFTYDLADCGHRYMQI
                     ERLLEYWQRVLPLPMLEVQYEQLVADLPGQTRRLIDFLGLAWDPACLEFHRTPRAMTT
                     ATAWQVRRPIYRDAVGRWRNYQRHLQPLLDVLGASGTGLSS"
     gene            complement(2366..3925)
                     /locus_tag="VLI90_03155"
     CDS             complement(2366..3925)
                     /locus_tag="VLI90_03155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV13229.1"
                     /translation="MDSQIDQLRTDYDAVPYLSDAFAASHPDNLRTIATLAGFAGAAD
                     VTRCRVLEIGCAIGRNLIPMAQTLPGSTFVGIDLSPKQIEIGKRTVSELGLANIELRV
                     RDLMDFGADEPAERFDYIIAHGIYSWVPAAVRDRLFEVCGPMLRPAGLAYISYLALPG
                     SQLRQIVRDLSAFHCRGAALDPLERAARTREAVAFAAQAAPGPQVYKSLLSGLDAAAK
                     RSSDWSFLHDELELVNHAVYLEQFVAEAARHQLRYVADAHPTQEIFPYLLPATQQQVR
                     QFSTDLVKRQQYSDYLEGRAYHASILCRADAGTKPEDLRFAADPQTRISLVGMHVAGR
                     IVEETPDGVAGAAPGTLRFGARGARVKLMSHDPLVISAYRTLRAAWPRAMPFEELLEA
                     CGGSSVDPQAAAETLTRNLMLAYYVGIVELWTRPTIFVGVDPQRPRVTPLARLEASTS
                     ASVVNLRHQPINPDAALRQLLQQMDGTRSRDELFELWMRTTGGGGVKDRATFDRLLAE
                     LVANSCVLPDA"
     gene            4557..4628
                     /locus_tag="VLI90_03160"
     tRNA            4557..4628
                     /locus_tag="VLI90_03160"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4589..4591,aa:Ile,seq:gat)
     gene            4708..4783
                     /locus_tag="VLI90_03165"
     tRNA            4708..4783
                     /locus_tag="VLI90_03165"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4744..4746,aa:Arg,seq:acg)
     gene            4875..4946
                     /locus_tag="VLI90_03170"
     tRNA            4875..4946
                     /locus_tag="VLI90_03170"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4907..4909,aa:Lys,seq:ctt)
BASE COUNT          917 a         1632 c         1561 g          862 t
ORIGIN      
        1 gtattcgcgc gcgagggtcg gggtgaggtg aattcgagca cgaatggtac gaaattctta
       61 gatcaccctc acccttttct atgaacaatc gctgctgtca atagcgttgg cacacgttgg
      121 cactgctggg gcactgcgtc cagggcaaac gcatgtagga accgcgcgct ctggtcccct
      181 ctcccggtac gccgggagag ggttagggtg agggtgattt cgagtgccag cgactatcga
      241 cattcgaaat caccctcacc cctgccctct cccggagacc tgcggtcagc agtaccggga
      301 gagggggaag gagctacatg cgattgccct gaccctctcc cggagtaccg ggcgagccgg
      361 atcagagcgc acgcctttaa tccagttcct attcgtggca acacgggcgc ccagcgcttc
      421 acaggttctg tctttctctt ccctgccgtc ttggcctctt ggcctcttgg cgctgacaga
      481 cccataccaa gatcgtgcgc tacacccttc gactggttca cgagcacgtg ctgcgctcaa
      541 gacgacaacc ccgtccccga tgcccccagc acatccagca gcggctgcag atgccgctgg
      601 taattccgcc agcggcccac cgcgtcgcgg taaatcggcc ggcggacttg ccacgccgtc
      661 gcggtggtca tcgcccgcgg cgtgcgatga aactcgaggc aggcggggtc ccacgcaagt
      721 cccaggaaat cgatcaaccg ccgggtttgc cccggcaggt ccgccaccag ctgttcgtat
      781 tgcacctcga gcatcggcag cggcaggacg cgctgccaat attccagcaa ccgctcgatc
      841 tgcatgtatc gatgcccgca gtcggccagg tcgtacgtga acaggtttcc gctggcgaaa
      901 tgctggaaga agcaggagag gcagttgtcg cgcggatcgc gacggcagat gatcacccgc
      961 gcgccgggga acatcgtggc gatcaacccg agctcgatcg cgttccccat catcttatcg
     1021 accacccgct gcgtctgaac ggcgccacct tcatcgccgc cgccgcgtgc agcgacatga
     1081 tcgcgcgcga tggcctgcag acgttccaga tgctgcgccg cggccgcggc gatcgccgcc
     1141 cgcgtccatc ccgcgggatc tctgccaagc cccgcggaaa tgcggtcgat gtctttcagc
     1201 tcacccgcgc caaccaccgc cgggtgactg gcggcaatct gttcgaccaa cgtcgtgccc
     1261 gagcgcggca tcccgacgat gaacacgggc aactgcgaag ggtttcccca atcgcggtgc
     1321 tcttggaaaa acgccggcgt gaaccgcgcg atgatctggt cgatttggcg gcgcagcaga
     1381 tcgctgtcga accgatcgcc atgcgccgcc gcctgctgct tgacgcgcgc gttcgcggtt
     1441 gcgaagtaac caaaggcatc atcgaagcgg ccggcttcat cgagcagttt gccgacggcg
     1501 aaccccgctc cgacgcgatc gagctctgaa gcatccggcc ggctcagcac ggccagcagc
     1561 ggagccacct gcgcatccac cgccgctccc gcggctttgg tgatgctcgc caggttggtg
     1621 gcgaacaagg cgatgtcggg ccgaagttcg ctggcgcgtg cgaagcagcg tgccgcctcg
     1681 tccaactgcc ccatcgcgcg cagcgcgctg cccagcgcgt tccacgccgc ggccagctgc
     1741 ggcgccagct cgaccgcacg ccgaagcgtg tcggtggctt catgcatttt cgccgggtgg
     1801 cgatggagga tgatcccctt cgcctggtag gccatcgcgt cgttcggggc aagctcgatc
     1861 gcgcgatcga gcgcccgcgt ggcctcgtcc acccggtcca gctcacccag cgtcaccccg
     1921 agcccaacca gcgctgccgc gaagttggga ttcagttgca ccgtccgcgc gtacgcagct
     1981 cgcgcttcct cgattcggcc gacgcgcagc agcgtttcgg cctggtggaa atgcacgagc
     2041 ggatcgttgg gctgcagctc ggctgccttc ttcaatgcat cggccgcttc agtaacccgg
     2101 cccagcgcag tcaacgccaa agccaggttc gtcagcgctt cgggatagcg cggcttgatc
     2161 cgcagcgcgc ggtgaatcag ctgcaccgcc gcatccggcc ggccttcctg cgcgttcacc
     2221 acgcccagca tgtgcagcgc atcggcgtga ttcggctcgg ccgcgagcac ctgctcgtat
     2281 gcagcccgcg ccgggcccag tcgccccgcc tgatgatggg ccaacgcctg ctggaaaaga
     2341 ttcgcttgcg gagtcggcat ggatttcacg catccggcag aacacagctg ttggcgacca
     2401 gttccgccag cagtcggtcg aacgtcgctc ggtcctttac accgccgccg ccggtggtgc
     2461 gcatccacag ttcgaacagc tcgtcgcggc tgcgcgtgcc atccatctgc tgaagcagct
     2521 gccgcagcgc cgcgtcggga ttgatcggct gatgccgcag gttgaccacc gatgcgctcg
     2581 tgctggcttc gagccgcgcc agcggcgtga cgcgcggccg ctgcggatcg acaccgacaa
     2641 aaatggtggg gcgggtccac agttcgacga tgccaacgta gtacgccagc atcaggttcc
     2701 gcgtcaacgt ctcagccgcg gcttgcggat cgaccgatga accgccgcac gcttcgagca
     2761 actcttcaaa cggcatggcc cgcggccagg cggcgcgaag cgttcgatac gcgctgatga
     2821 ccagcgggtc atgcgacatc agcttcaccc gggcgccgcg cgcgccgaag cggagtgtgc
     2881 ccggagcggc gccggcgacg ccgtccgggg tttcctcgac gatccgaccc gccacgtgca
     2941 tgcccaccag cgagatccgc gtttgcggat ccgcggcgaa gcgcagatct tccggcttcg
     3001 tcccggcgtc cgcgcggcaa aggatcgacg cgtgatacgc ccggccttcc agataatccg
     3061 aatactgttg ccgcttgacc aggtccgtag agaactgccg cacctgctgc tgcgtcgccg
     3121 gcagcaggta gggaaagatt tcctgcgtcg ggtgtgcatc ggccacgtag cggagctggt
     3181 ggcgcgcagc ttcggccacg aactgctcca ggtacaccgc gtgattgacc agctccagct
     3241 catcgtgaag gaacgaccag tcgctggaac gcttggccgc cgcgtccaat ccgctcagca
     3301 gcgacttgta cacctgcggc cccggcgccg cttgtgcggc gaacgccacc gcttcgcgcg
     3361 ttctcgctgc gcgttccaac gggtcaagcg ctgcgccccg gcagtggaac gccgacagat
     3421 cacgaacgat ctgcctcagt tgcgagcccg gcagcgccag atagctgatg tacgccaggc
     3481 cagcgggcct cagcatcggc ccgcacacct cgaacagccg atcgcgaacc gccgccggca
     3541 cccaggagta aatcccatgt gcaatgatgt agtcgaaccg ctccgctggc tcgtccgcgc
     3601 cgaagtccat caggtcccgc acgcgcagct cgatgttggc cagtcccagc tcgctcaccg
     3661 tgcgcttgcc gatctcgatc tgcttcggcg acagatcgat gccgacgaac gtgctgccgg
     3721 gcagcgtctg ggccatcggg atcaggttgc gcccgatcgc acagccgatc tccagcaccc
     3781 ggcagcgcgt gacatcagcg gcgcccgcga agcccgcgag cgtggcgatc gtgcggagat
     3841 tgtccggatg cgatgccgcg aacgcgtcgc tcaggtatgg cacggcgtca taatccgtgc
     3901 gaagctggtc gatctgtgaa tccatcggtc gaagctcccg caaaagaaat gatcgccaca
     3961 ccaccggcga atgcaagtgc ggggaaccat cgagatttgc gccagcgcag gcggagcact
     4021 ccatagatcg gttcggaatt tttctcttgc atttcatagt tggcgcggaa taattgaacg
     4081 catcgacctg ttatgtcgat ggagagacac atgcggcaca gttggaagca acccgaaacg
     4141 ctatgcgata cctcacgagg ttatctcgtg attaaccacg tcgcggcgaa gccgcgcaga
     4201 tagctcccgc cttttgttgg ccgggagttc ttcccggtcg aaagtaagcc ctttttgctt
     4261 gaaaaccggg aaaacgtgac gcggatgaac cgcgcacttg ttttgactgc gggcctgtag
     4321 ctcagcggcc agagcgccgc ggtcacaaga gcggaggttg cgggttcgag tccagcttgg
     4381 cgcacggtag ttcgagtagc ggtcatgtat gaaaagggag gatcgattga aaagacgcaa
     4441 ctgtgacacg ggcttccagc ccgtgcgagc gacgtagaag tagagaaatc tatagtgtgc
     4501 tcaagttaga acgcaagatg cacgggccag aggcccgtgt cacagggaga ttgtgcgcgc
     4561 cgttagctca gaggcagagc acccgcccga taagcgggag gccggaattt caatacttcc
     4621 acggcgcact gacaaagacg tagcacgggc gtctcgcccg tgccgagcga tccaggcacg
     4681 ggcgagacgc ccgtgctacg tgaaaacgca ttggtagctc aatcggaatc agagcgccgg
     4741 tctacgaaac cggaggttga aggttcaatc ccttcccagt gcactgaacg gacgaagtga
     4801 ttgtatctag tatcgaagcg atagggagcc gcggccgccg ggggggcggc ctgcggcagc
     4861 gtgggacaag cgccgcactc gtagctcaat ggcggagccg ccgcctctta agcggtggga
     4921 tgcaggttcg agtcctgccg ggtgcatgga aggggtcagg agtcagtaaa ga
//
LOCUS       DATIMH010000187         5657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_41742, whole genome shotgun sequence.
ACCESSION   DATIMH010000187 DATIMH010000000
VERSION     DATIMH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41742"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VLI90_03175"
     CDS             <1..214
                     /locus_tag="VLI90_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13230.1"
                     /translation="EHHWTILGVGNRPGVRVMILGCCMIVAGVLYAFYAKPYIIRRMK
                     ANAIAKANALKAAKAAAPKPVELVNS"
     gene            267..2129
                     /gene="ccsA"
                     /locus_tag="VLI90_03180"
     CDS             267..2129
                     /gene="ccsA"
                     /locus_tag="VLI90_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSV13231.1"
                     /translation="MRSQLGRVLLVTFALLAFSPVARADGADDAFAKAVDLSPLRTVA
                     VQHRQTLKTLDSYARQTISAITGHSSIDGHDAIFSILDIAYHPDDYAKLNLIKIRNVP
                     LRQEFRGLKLISSDEAERIVHDGTISLNLWMQDDVQQMLRDVMASDTRKSNAVDQAAE
                     AAGAMSELCRGTDPFVPVACIPPATNSADDHIWHTFSQTAGNSSIWTAVAKQNHGAVI
                     APLPNYAADRIDACVRYSFLMMQAWRDKNAADVNSNATALAAALEQVNPAVYPPMIKR
                     QVEVVYNRLAKMTLPGAAFYFLAFVLFLAFARSGVRSLHLWGLRFFAIGFLIHTIGIA
                     IRWWLVGSIPIKNQFESVMFSAWFGCAVGLILELRRPRGIFGAGASFVGWLSLVAIFC
                     APFVFGREIGGEIGQVNGVLMSYWLYIHVTAVTASYSLIGTAFLLSAWWLIRYYRSQD
                     ELGDTSGHRLSADAAEPDLRDVSVSTSGGGAVVQSSWLNSLAAVLFIPGATKTTAKPR
                     AALPVAEKHRVFLANLDACNLVVLQLAFWILGAGIIFGAIWADESWGRPWGWDPKETF
                     ALVTWIVYLIVVHVRVATEDKAWWTAVLSVIGFFIMLFNWIGVNFFLVGLHSYA"
     gene            2198..2839
                     /locus_tag="VLI90_03185"
     CDS             2198..2839
                     /locus_tag="VLI90_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13232.1"
                     /translation="MSDPIVIAYHLIWTAYGWWLPNDIRGSGSTIIRNDIHKKLGELH
                     LGRKQSQPAGWQVRDFYQQASDLLKYPLLTFGPAQVNAIASPFQQVIQQMKYTCYACA
                     IMPDHIHLLIRKHKHLAEEMIGNFETAGRATIAGTGGWARSHPVFTLGGWKVYLDHPD
                     EVWRTIPYIEENPGKIGLPRQQWSFVTPYNNWPLHEGHSPNSPYARRLREYRR"
     gene            complement(2847..4439)
                     /locus_tag="VLI90_03190"
     CDS             complement(2847..4439)
                     /locus_tag="VLI90_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13233.1"
                     /translation="MGIIEQLEDRALLSVVLTGMRNIGQVDLPTGVPAQYSGAIDQTT
                     GFGYFGSASGANPGIVSKINLNGPLPTVVQDGLPNSVPLNVGHFNAIEIDTTAADATQ
                     HYLYVAGNSGHILKMSPGDATHDPQVAAQLAATSASGGILGGAIDTSNVDPTQRYAYF
                     LVMETSDSKLLRVRLSDFSDQGSETLNMGNARTCAIDTIHHYLYCTTYPSTTVTVPQI
                     CKVNLATFTPGGSSTATCSMGSTTPGDGIAIAGYDNPGFDIGLSIDPVHQFAYLGSFN
                     ATSDASKSQTYPYNQSIIVRVNLGNGDAFPAHPISVLKLGIGEQDLTASVIDLAHGNV
                     YFGTDLTYPGHVYKIHVGDGTQPMTEIGRLDLNLGTAGSYPPDGTPGGIPNNTPENGG
                     EIYLRSAVFNATQNAIYFGTDGRPGQIIKVATALGGDANVDGMVDVADLGALASHYGT
                     TSGAVWAQGDFDWNGTVDVADLGALATNYGASLPSGAASAAMAASNQPIDSASGAPTP
                     DFSVQPADFDWLFRKKSRPLAA"
     gene            complement(4588..5556)
                     /locus_tag="VLI90_03195"
     CDS             complement(4588..5556)
                     /locus_tag="VLI90_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSV13234.1"
                     /translation="MSEAAAVAGEGEGSRRQRRGDRLQRREHRRRRFRRHGRRAYIRS
                     VYFLPSMATLGNAICGFGAMYVAALAPDIHSRDRLTNFFSLHHFTVAAYLIFVAMIFD
                     ALDGRLARFARHTTDFGGQLDSMADVVSFGVAPAYIALLLFKAGTEQLPPPTIISRLI
                     WAIGALYMSCAAIRLARFNVSNEHGEQHHFSFLGLPSPGAGGTVAAWVLMQQDLANTA
                     FEMGADSRIGRLLGHCSTLCILVLPLIVLMTGLLMVSNIRYPHLVNRYLRGRRSIGRL
                     MAVLIVVLLIVVAHQYVLAMGTMVYALWGLMSATNRRIRRRPTPVV"
BASE COUNT         1044 a         1774 c         1779 g         1060 t
ORIGIN      
        1 agagcaccac tggaccatcc tcggcgtcgg taaccgtccg ggtgtgcgcg tgatgatcct
       61 cggctgctgc atgatcgtcg ctggtgtgct gtacgcgttc tacgccaagc cgtacatcat
      121 ccgaaggatg aaggcgaacg cgatcgccaa agcgaacgcg ctgaaggcgg cgaaggcggc
      181 agcgccaaag cccgtcgagt tggtgaattc gtaatagcgt agcccatgct acaaatctgc
      241 tcgtggtgtt tcacatgatg aaagctttgc gttctcagtt gggccgagtg ctgctcgtca
      301 cgtttgcgct gctcgcgttc tcgcccgtcg cgcgcgctga tggcgctgac gatgccttcg
      361 ccaaagccgt cgatctctcg ccgctgcgca cggtcgccgt gcagcatcgc cagacgctca
      421 agacgctcga cagctacgcg cgccagacga tctccgccat caccggccac tcgtccatcg
      481 acgggcacga tgcgatcttt tcgatcctcg acatcgccta ccaccccgac gactacgcga
      541 agctgaacct gatcaagatc cgcaacgtcc cgctgcggca ggaatttcgc gggcttaagt
      601 tgatcagctc tgacgaagcg gagcgaatcg tgcacgatgg cacgatctcg ttgaacctgt
      661 ggatgcagga tgacgtgcag cagatgctgc gggatgtgat ggcgtcggac acgcgcaaga
      721 gcaacgcggt ggaccaggcc gccgaggcgg ccggggccat gtcggaactg tgccgcggga
      781 ccgacccgtt cgtgcccgtc gcgtgcatcc cgccggcgac gaatagcgcg gacgatcaca
      841 tctggcacac cttcagccag accgccggca attcatcgat ctggacggcg gtggcgaaac
      901 agaaccacgg cgccgttatc gctccgctgc cgaactacgc ggccgatcgc atcgatgcct
      961 gtgtgcgcta ttcgttcctg atgatgcagg cgtggcgcga caagaacgcc gccgatgtga
     1021 actcgaacgc caccgcgctg gcggcggcgc tggagcaggt gaacccggcc gtttatccgc
     1081 cgatgattaa gcggcaggtc gaggtcgtct acaaccgcct ggcgaagatg acgctgccgg
     1141 gggcggcgtt ctatttcctc gcattcgtgt tgttcctggc cttcgcgcgc agcggtgtgc
     1201 gatcgctgca cctgtggggc ctgcggttct tcgccatcgg ttttctcatc cacacgatcg
     1261 gcatcgccat ccgctggtgg ctggtcggca gcatcccgat caagaaccaa ttcgagagcg
     1321 tgatgttcag cgcgtggttc ggctgcgcgg tcggattgat cctggaattg cgccgcccgc
     1381 gcggcatctt cggcgctggc gccagcttcg tcggctggct gtcgctggtc gcgatcttct
     1441 gcgccccgtt cgtcttcggc cgcgaaatcg gcggcgaaat cggccaggtc aacggcgtgt
     1501 tgatgagcta ctggctctac atccacgtca ccgcggtgac ggcgagttac tcgctgatcg
     1561 gcactgcgtt cctgcttagc gcgtggtggt tgattcgcta ctaccgcagc caggacgaac
     1621 tgggcgacac ttcaggacat cgactatcag ctgacgccgc cgagccggac ctgcgcgacg
     1681 tgagcgtcag cacgagcggc ggcggtgctg tcgtgcaaag cagctggctc aactcactcg
     1741 ccgccgtgtt gttcattcca ggagcgacga aaacaactgc taaaccgcga gcggcgttgc
     1801 cggtcgccga gaagcaccgc gttttcctcg ccaacctcga cgcctgcaac ctcgttgtcc
     1861 tgcaactcgc gttctggatc ctcggcgccg gcatcatctt cggcgccatc tgggccgacg
     1921 aatcctgggg ccgcccctgg ggctgggacc ccaaggaaac cttcgcgctg gtgacgtgga
     1981 tcgtttacct catcgtcgtg cacgtccgcg tcgccacgga agacaaagcc tggtggaccg
     2041 cggtgctgag cgtgatcggc ttcttcatca tgctcttcaa ctggatcggc gtgaacttct
     2101 tcctggtggg cctgcacagt tacgcgtgat cagccgcttg cggcttagcg tcttttgttc
     2161 ttccaattgc aggacggcgc cgatatcgtc gcgcgccatg tccgacccca tcgtcatcgc
     2221 ctatcacctc atctggacgg cctacggttg gtggttaccc aacgacattc gcggcagcgg
     2281 ctcaacgatc attcgcaacg acatccacaa aaaactcggc gaacttcatc ttggccgaaa
     2341 gcagtcgcaa cccgcgggtt ggcaagttcg agacttctat cagcaagcga gcgatctgct
     2401 gaagtacccg ctgctgacgt tcgggccggc gcaggtgaat gcgatcgcca gtccatttca
     2461 gcaagtgatt caacagatga agtacacctg ctacgcctgc gcgatcatgc cggatcacat
     2521 ccatctgctg atccggaagc acaagcacct ggcggaggag atgatcggca actttgagac
     2581 cgcgggacga gcgacaattg cgggcacggg cgggtgggcg cggagccatc ctgtcttcac
     2641 gctgggcgga tggaaggtgt atctggatca tccggatgaa gtgtggcgga cgatcccgta
     2701 catcgaggag aatccgggga agattggtct accgcggcaa cagtggagct ttgtgacgcc
     2761 gtataacaac tggccgttgc atgagggcca cagtccgaat tcgccgtacg cgaggcggtt
     2821 aagggaatat cgaaggtgaa cagacgctaa gccgcaagcg gccgggattt tttgcgaaac
     2881 agccaatcga agtcggcggg ttgtaccgag aagtccggag ttggagcgcc agacgccgag
     2941 tcaatcggct ggttcgacgc cgccatcgct gccgacgccg cgccactcgg caaactcgcg
     3001 ccatagttgg tcgccagcgc gcccaaatcc gccacgtcca ccgtcccatt ccaatcgaag
     3061 tccccttgcg cccacaccgc gccgctggtc gtgccatagt gcgacgccag cgcgcccagg
     3121 tccgccacgt ccaccatgcc gtcgacgttc gcgtcacccc cgagcgcggt cgcgaccttg
     3181 atgatctggc ccggccggcc gtcggtgccg aagtagatcg cgttttgcgt cgcattgaag
     3241 acggcgctgc gcaggtaaat ttcaccgccg ttctcgggcg tgttgtttgg aatgccgccg
     3301 ggcgtgccat ccggtggata gctgccggcg gtgccgaggt tcaggtccag ccggccgatc
     3361 tcggtcatcg gctgcgtacc gtcgccgacg tggatcttgt acacgtggcc gggatacgtc
     3421 aggtcggtgc cgaagtagac gttgccgtgg gcgaggtcaa tcaccgacgc ggtcaggtcc
     3481 tgctcaccga tgcccagctt caacacggaa atcggatgag cggggaacgc gtcgccgttg
     3541 cccaggttca cgcgcacgat gatcgactgg ttatacggat aggtttggct cttgctcgcg
     3601 tcggatgtcg cgttgaacga gccgaggtac gcgaactgat gaacgggatc gatgctgagg
     3661 ccaatgtcga accccggatt gtcataccca gcgatggcga tgccatcgcc gggcgtcgtg
     3721 ctgcccatgc tgcaggtcgc ggtgctggac ccgccgggcg tgaaggtggc gagattcacc
     3781 ttgcagatct gcggcaccgt cacggtcgtc gagggatagg ttgtgcaata gagataatgg
     3841 tgaatcgtgt cgatcgcgca cgtgcgcgcg ttgcccatgt tgagcgtctc ggagccttga
     3901 tcggaaaaat ccgacaggcg aacgcgcagc agcttcgaat cggaggtttc catgacgagg
     3961 aagtacgcgt agcgctgcgt gggatcgaca ttgctggtgt cgatcgcgcc gccgaggatc
     4021 ccgcccgatg ccgacgtggc ggccagctgc gcagccacct gcggatcgtg cgtcgcgtcg
     4081 ccgggtgaca tcttcaaaat gtggccgctg ttccccgcca cgtacaaata atgctgcgtt
     4141 gcatccgcgg cggtcgtgtc gatctcgatc gcgttgaagt gcccgacgtt cagcggaacc
     4201 gagttaggca atccatcctg caccaccgtc ggcagggggc cattgagatt gatctttgaa
     4261 acgatgccgg ggttggcgcc gctggcagag ccgaagtaac cgaagccggt cgtttgatcg
     4321 atcgcgccgg agtattgcgc cggcacgccc gtcggcagat cgacctgccc gatgttgcgc
     4381 atgccagtca gcacgaccga caacagtgca cgatcttcca attgttcgat gatgcccaag
     4441 ggcagctccc gcttgcagat tatgcgcagt gtccaccgcg cgcgcaatcg cgcgccacaa
     4501 aaaaacgcca cggctcggcc gtgggacagc ttctcgaaaa aaccgtccca cggcagaacc
     4561 ctgggctttt gggcaaaatt tgacgtttca aacgaccggc gtcggtcggc gccgaattct
     4621 tcgattcgtc gccgacatca atccccacag cgcatacacc atcgtcccca tcgccagcac
     4681 gtattggtgc gcgacgacga tcagcagcac gacgatcagc accgccatca gtcggccgat
     4741 cgagcgccgg ccgcgcagat agcggttgac caggtgcgga tagcggatgt tgctgaccat
     4801 cagcaggccg gtcatcagca cgatcagcgg cagcaccagg atgcacaagg tcgagcagtg
     4861 gccgagcaat cgcccgatgc gggaatcggc gcccatctcg aaagcggtgt tggccaggtc
     4921 ctgctgcatc agcacccacg cggccacggt gccgccggcg ccggggctgg gtagtcctaa
     4981 gaagctgaag tgatgctgtt cgccgtgttc gttgctgacg ttgaatcgcg ccaggcggat
     5041 ggcggcgcag ctcatgtaca gcgcgccgat cgcccagatc aatcgcgaga tgatcgtcgg
     5101 cggcgggagc tgctcggtgc cggccttgaa cagcagcagc gcgatgtacg ccggggcgac
     5161 gccgaagctg acgacatcgg ccatgctgtc gagctgccca ccgaagtccg tcgtgtgtcg
     5221 cgcgaagcgc gccagccggc catcgagggc atcgaagatc atcgcgacga agatgagata
     5281 cgcggcaacg gtgaaatggt gcagcgagaa aaagttcgtc agtcgatccc ggctgtgaat
     5341 atcgggcgcc agcgccgcga catacatcgc cccgaatccg cagatcgcgt tgcccaacgt
     5401 cgccatgctt ggcaggaaat agaccgagcg gatatatgcc cgccgcccgt gccggcggaa
     5461 gcgccgccgg cggtgctcgc ggcgttgcag ccggtccccg cggcgctgtc gccgcgatcc
     5521 ctcaccctcg cctgccaccg ccgccgcctc gctcatgcgg gtgtctcccg gccgacgatc
     5581 ggttcgacct ccgggtccac gatgtgcgtc ctggtatgaa tcggctcagt caaaatcgca
     5641 atgatgtccg ccgcccc
//
LOCUS       DATIMH010000188         9488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3218210, whole genome shotgun sequence.
ACCESSION   DATIMH010000188 DATIMH010000000
VERSION     DATIMH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3218210"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VLI90_03200"
     CDS             <1..927
                     /locus_tag="VLI90_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV13235.1"
                     /translation="SDLLVSLSSSNQTAPNFWLDPAVNVQRSVYVQTPQYKIASINEL
                     QNTPVTPTNAAAKNGDVQLLENLASVSRGTSPTNVTHYDIAPTRDVLLGVEGTDLGSA
                     AKRIQQVVDKYAKQLPRGSFIKIRGQVQSMNESFYAMAGGLVLAVVLVYLLMVINFQS
                     WMDPLIILMALPGALAGILWMLWATGTTVSVPALMGAIMSIGVATANSILMITFANDQ
                     RAEGKDARDAALAAGMTRLRPVMMTALAMIIGMLPMSLGLGEGGEQNAPLGRAVIGGL
                     SMATFATLLFVPVVYSLLRKNAPVTQVEEELR"
     gene            927..2261
                     /locus_tag="VLI90_03205"
     CDS             927..2261
                     /locus_tag="VLI90_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSV13236.1"
                     /translation="MTTTERTMDRPDTRDNHGNGEATRDVHAGHEEIPKNLPRPSNAT
                     VIVVLGVFVLLLVALFFVGYIPHRHRVAEADADAAERASGVPVVQVELPKKSAPTSDL
                     LLPCDIRANQETAIYPRSSGYLKKWYVDIQDKVQAGQLLAEIDAPEVDAQLAQSRAQL
                     EQSKANVVRANSDLDLAQKNLDRFEEANKNSPGSVTQQQLDQMRAAVENTRSALEQAK
                     ASVASSDADVQRLSVLQNFERVTAPFAGIITSRNYDVGALLNPSDTGAGRQMFSIAQI
                     DALRVFVNVPQAESTEVKVGQPVFLKVRNYPDREFAGEVARTAGSIDPATRTMTVELH
                     FPNKDLALVPGMYGQVRLPLHEAAPTLTIPTSALVFNADGLRADVVRDGKIHEQKLQV
                     GRDFGTEIEVSSGLSDQDEVVTNPGEKLSEGVEVKTFEPKQAAPNTRVAHAN"
     gene            complement(2324..3181)
                     /locus_tag="VLI90_03210"
     CDS             complement(2324..3181)
                     /locus_tag="VLI90_03210"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HSV13237.1"
                     /translation="MDDIATLKNVQLFASLGAADVQTLRAAMGEQRFVPGQVIIRSGE
                     PGDRFYIITEGRVQFVSADADGNEVVVGDAGAGGFFGELSMLTGEPRTVRVRALDAVR
                     TLTLGRDDLHGFLRSHPEAAISVLTEIARRLHKTDSMLRNSTSRNVNDIADDKATFGQ
                     RIADAVAAKMGSWTFIIVQSVLLAIWVTLNCLAWVFHWDPYPFILLNLALSFQSAYAA
                     PIIMMSQNRSSDKDRLAAEIDHQVNVKAEIKTGQIISRLDDLERGMHALHQEHMSMLR
                     GGGGQAAAS"
     gene            3284..3556
                     /locus_tag="VLI90_03215"
     CDS             3284..3556
                     /locus_tag="VLI90_03215"
                     /inference="COORDINATES: protein motif:HMM:NF017793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HSV13238.1"
                     /translation="MAQANVDPVELRRFASDLNRFNGELQALMSGLHGRLRGLENTWR
                     DQEQRRFTEVFEQTMKVLNVFLENSHLHASFLSKKAAAIEAYLKQR"
     gene            3632..4702
                     /locus_tag="VLI90_03220"
     CDS             3632..4702
                     /locus_tag="VLI90_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13239.1"
                     /translation="MAQQGARVGSVDAIRQFRAALIKFAESGNVALTSADTDVERTLS
                     WLEREQTVYWANQVRKRHEFVQRCEDAVRQKRLFKGFDGREQSAVDEMKALSNARRMR
                     EEAEQKVLAVKRAIQTLQKEAQLYKGRVQKLSTTLQQDIPNAVRMLDAMVGQLEGYLA
                     VQTEGAGIGLAEAAESVAKAAGPRRVGYERLRDLTPKPEQRAQTPPKALTADDAFFKP
                     WVPGVVEKWQHEALAKLSIDRLPLDPEQKVIIAHNCWQQPRIYLEHLDPAFEGDSGWF
                     IGSPEEGELAHEAFDAIRLGDVIAAREDLADILSLPPGFLLVLDSGGVAAILDAAGLD
                     VWAVALMTDAAKNPSAAAPAGS"
     gene            4706..4918
                     /locus_tag="VLI90_03225"
     CDS             4706..4918
                     /locus_tag="VLI90_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13240.1"
                     /translation="MGLQESRGKLARSMKDLLAQWALVRSQWNDVNAERFEREVLHML
                     EMDLRNASGAMDQMGALLAQIRSQCD"
     gene            5097..9086
                     /locus_tag="VLI90_03230"
     CDS             5097..9086
                     /locus_tag="VLI90_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HSV13241.1"
                     /translation="MPDPLDESLVTLDRLPPTAAPAAASAGAAPPATAETDRDIQRSA
                     LRDLIALSTESASTEADIEREHRTSLEKEKQTFSDRDFQINEQHKQHVADANQQNQDR
                     LNELASQYQAERNKIVSADKAFRQKIESEKSAIDDRLKQKYDQAVWEADSVNEMASQK
                     IAADYKATTEAIKAAGDALNAMEVKAAELVQLYGLPLATDAVNVDDDPQITTSTEVAF
                     ARHKEAIEQHLSRLGHMPLASLFIGIRPYLFLLLITAAAAAAVQFHAQSTDPQWKALA
                     IFAGGTFVLALAIGFALRLLANSKVRAVYHPLRQELVLARSAAEQQQANAVEKRKADT
                     AAATKARQDEVQAAKEEVGPQAAKAARNRDAATATAQAEFAKQVARIDRIRDTANAET
                     AERLRRMIHEIDHKQERELRDNKTRNQTRLVEIEQRYQQRRAELEQHWSERLRRIQAP
                     IDRDGDSVPASLDWNDGAWKSWTPPKKFASRVRFGEMQVDLKQIAENVPQRLTLPPTF
                     SLPALLAFPAQASLLIHTDHAGRGEAIRTLQMLMARLLTNLPPGRVRFTILDPVGLGQ
                     NFAGFMHLSDYDDALVGGRIWTSQDQIDQRLANLTDHMETVIQKYLRNEFQTIDEYNA
                     QAGELAEPYRFLVIADLPVGFTSEAFRRLNSIASTGARCGVYTLIARDTRAALPAGAH
                     LDELVAHSVNLVREGDRFVWDDDVFRPLPLTLDPPPREDVLLTEILHKVGAGAKEAKR
                     VEVPFENIAPSNEAIWSRSTTSEIDVPIGRLGANRLQSLKLGRGVAQHVLIAGKTGSG
                     KSTLLHAMITNAAMWYSPDEVELYLIDFKKGVEFKTYASNLLPHARAIAVESDREFGL
                     SVLQRLDAELSRRGELFRKAAVQDLAAYSKTPNAVVIPRTLLMIDEFQEFFSEDDKLA
                     QESALLIDRLVRQGRAFGIHVLLGSQTIGGAGGLPRSTLGQMAVRIALQTSEADSQLI
                     LGDNNSAARLLSRPGEAIYNDAGGLVEGNSPFQVAWLPDDKRDKYLRDLARRAKPYFE
                     RFGYPIVFEGNAPADIRRNPQLTAAMEAKSWSRVAAARAWLGEPVAIKEPTAVTFRRQ
                     AGANMIMVGQQDEQSMALACSSIVSLAAQQGSDQAVFYILDGTPADSALAGTFARVGA
                     ALPHDVKLVEWRATPEAIDEIAKEVTRRQDVDQPGAPSIYVIVYGLQRYRALRKQEDD
                     FGSFSMDADEPKKANPGKQFAEILRDGPPVGVHVITWADTLVAVDRTLDRGSMREFDN
                     RVLFQMSAADSSNLIDSPVGNKLGFNRALVYSEEQGVMERFRPYALPDGAWLATVSSL
                     LNARNTQPA"
BASE COUNT         1880 a         3065 c         3019 g         1524 t
ORIGIN      
        1 agcgatctgc tggtctcgct cagctcgagc aaccagacgg cgccgaactt ctggctggac
       61 ccggcggtga acgtgcagcg cagcgtttat gtgcagacgc cgcagtacaa gatcgcctcg
      121 atcaacgaac tgcagaacac gccggtaaca ccgacgaatg ccgcggcgaa gaatggtgat
      181 gtccagctgc tggagaacct ggccagcgtg agccgcggga cgtcaccgac gaacgtcacg
      241 cactacgaca tcgcaccgac gcgcgacgtg ctgctgggcg tggaaggcac cgacttgggt
      301 tcagcggcta aacgcatcca gcaggtggtg gacaagtacg ccaagcagtt gccgcgcggg
      361 tcgttcatca agatccgcgg gcaggtgcag agtatgaacg aatcgttcta cgcgatggcc
      421 ggcgggttgg tcttggcagt tgtactggtc tatctgctga tggtgatcaa cttccagtcg
      481 tggatggacc cgctgatcat cctgatggcg ctgccggggg cgctggccgg catcctgtgg
      541 atgttgtggg ccaccggcac caccgtcagt gtgccggcgc tgatgggggc gatcatgagc
      601 atcggcgtgg cgacggcgaa ctcgatcctg atgatcacct tcgccaacga tcagcgcgcc
      661 gaaggcaaag acgcgcgcga cgctgccttg gcggcgggga tgacgcgatt gcggccggtg
      721 atgatgaccg cactggcgat gatcatcggc atgctgccga tgtcgctggg cctgggtgaa
      781 ggcggcgagc aaaacgcgcc gctcggccgg gcggtcatcg gcggactttc aatggcaacc
      841 ttcgccactc ttctgtttgt tccggttgtt tacagcctgc tgcggaaaaa tgccccggtt
      901 acgcaggtgg aagaggagct aagataatga cgaccaccga gcgaaccatg gaccgacccg
      961 acacgcgcga caaccacggc aacggcgaag cgacccgcga cgttcacgcc gggcatgagg
     1021 agatccccaa gaacctgccg cggccgagca acgccacggt gatcgtggtc ctcggtgtgt
     1081 tcgtgctgct gctggtggcg ttgttcttcg tcggttacat cccgcaccgc catcgcgtcg
     1141 ccgaagccga cgccgatgcc gccgagcgcg cgtcaggcgt gccggtcgtt caagtcgagc
     1201 tgccgaagaa gtcggcgccg accagcgacc tgctgctgcc gtgcgacatc cgcgccaacc
     1261 aggagacggc gatctatccg cgctccagcg gttatctgaa gaagtggtat gtcgatatcc
     1321 aggacaaggt gcaggccggg cagttgctgg ctgagatcga cgcgccggag gtggacgcgc
     1381 agctggcaca gagccgggcg cagctggagc aatcaaaggc gaacgtcgtt cgcgccaact
     1441 cggacttgga tttggcgcag aagaacctcg atcgtttcga agaggcaaac aagaacagtc
     1501 ctggcagcgt aacgcagcag cagctggacc agatgcgggc cgccgtcgaa aacacccgca
     1561 gcgcgctgga gcaggccaag gccagcgtcg cctcgtccga tgcggatgtg cagcgcctct
     1621 cagtcctgca gaacttcgag cgcgtaaccg cgccgttcgc cggcatcatc acgtcgcgca
     1681 attacgacgt cggcgccttg ctcaatccat ccgacaccgg cgccggccgg cagatgttca
     1741 gcattgcgca gatcgatgcg ctgcgcgtct tcgtgaacgt gccacaggcc gagtccaccg
     1801 aggtgaaggt cggccagccg gtcttcctga aggtgcggaa ttatcccgat cgcgaattcg
     1861 ccggtgaagt cgcccgcacc gccgggtcca tcgatccggc gacgcgaacg atgacggtcg
     1921 agctgcactt cccaaacaag gacctggcgc tggtccccgg catgtatggc caggtccgcc
     1981 tgccgctgca tgaagcggcg ccgacgctga cgattccaac cagcgccctg gtcttcaacg
     2041 ccgacggcct gcgcgccgac gtggtgcgcg acgggaagat ccacgagcag aagctccagg
     2101 tcggccgcga ctttggcacc gagatcgaag tgtcatcggg attgtccgac caggacgaag
     2161 tggtcaccaa ccccggcgag aagctaagcg aaggcgtgga agtgaagaca ttcgaaccga
     2221 agcaagcggc gccgaacacg cgcgtcgcgc acgcgaattg acgccgcttg cggcttcgca
     2281 aaccacgcca ctcggtctgc gaagccgcaa gcggcaccac ggctcaggat gccgccgcct
     2341 gcccgccacc gccccgcagc atcgacatgt gctcctgatg caacgcgtgc atcccgcgtt
     2401 ccaaatcatc cagccggctg atgatctgcc ccgtcttgat ctccgccttc acgttcacct
     2461 ggtgatcgat ctccgccgcc aggcggtcct tgtccgacga ccgattctgg ctcatcatga
     2521 tgatcggcgc cgcgtatgcc gactggaacg acagcgccag gttcagcagg atgaacgggt
     2581 acgggtccca gtgaaacacc cacgccaggc aattgagcgt gacccagatg gccagcagca
     2641 ccgactgcac aatgatgaac gtccacgacc ccatcttcgc cgcgaccgcg tcggcgatgc
     2701 gctggccgaa cgtcgccttg tcgtcggcaa tatcattcac gttgcggctc gtgctgtttc
     2761 gcagcatcga atcggtcttg tgcagtcgcc gcgcgatttc cgtcagcacg ctgatcgccg
     2821 cctcggggtg gctgcgtaga aacccgtgca gatcatcgcg gcccagcgtc agcgtgcgca
     2881 ccgcgtcgag cgcgcgcacg cgcacggtac gcggctcccc cgtcagcatc gatagttccc
     2941 caaaaaatcc acccgccccc gcgtcgccga cgacgacttc gttcccgtcc gcatccgccg
     3001 agacgaactg cacgcggcct tcggtgatga tgtaaaagcg atcccccggc tcgcccgacc
     3061 gaatgatcac ctgccccggc acgaaccgct gctcgcccat ggccgcccgt aacgtctgaa
     3121 cgtctgcagc acccagcgac gcgaacaact gcacgttctt cagcgtggcg atgtcatcca
     3181 taggcgccag ttttcagcgg ctcgttgtct attgtcaatt gcatcgtcgg cgactgacaa
     3241 aagaccgtcc atcgctaaaa tgcagtagaa cggaggacca gtaatggccc aagccaatgt
     3301 agacccagtc gaattacgcc gcttcgccag cgacctgaat cgattcaacg gtgagctcca
     3361 agccctgatg tccggcctgc acggccggct ccgcggcctg gaaaacacct ggcgcgacca
     3421 ggaacaacgc aggttcaccg aagtcttcga gcagacgatg aaggtgctca acgtcttcct
     3481 cgaaaattcg catctgcacg cgtcgttcct gtcgaagaaa gccgccgcga tcgaagcgta
     3541 tttgaagcaa cgataagagc gcgaccgccg cttgcggcat cgcaaaccgg gcggcgtggt
     3601 ctgcgaagcc gcaagcggct caagtgacga gatggctcaa caaggcgcac gagttggatc
     3661 cgtcgacgcc atccgccagt tccgggcggc gttgatcaag ttcgccgagt ccggcaatgt
     3721 cgcgctcacc agcgccgaca ccgacgtcga gcgcaccctt tcctggctcg agcgcgagca
     3781 gaccgtctac tgggcgaacc aggtccgcaa gcggcacgag ttcgtgcagc gctgcgaaga
     3841 cgccgttcgg cagaagcggt tgttcaaagg cttcgacggc cgcgagcaat ccgcggtcga
     3901 tgagatgaaa gcgctgtcca acgcccggcg gatgcgcgag gaggcggagc agaaagtgct
     3961 cgccgtgaag cgcgcgatcc agacgctgca gaaggaagcg cagctgtaca agggccgcgt
     4021 gcagaagctg tccacgacgc tgcagcagga cattccaaac gccgtgcgca tgctcgacgc
     4081 gatggtgggg cagttggaag gatatctggc ggtgcagacc gaaggcgccg gcatcggcct
     4141 ggccgaagcc gccgaaagcg tcgccaaggc tgctggacct cgccgggtgg gctacgagcg
     4201 cctgcgcgat ctgacgccca agcccgagca gcgcgcccaa acgccgccaa aggcgctgac
     4261 cgccgacgat gcgttcttca aaccctgggt ccctggcgtc gtcgagaagt ggcagcacga
     4321 ggcgctggca aaactgtcga tcgatcgcct gccgctcgat ccggaacaga aggtgatcat
     4381 cgcgcacaat tgctggcagc agccgcggat ttatctcgag catcttgacc cggccttcga
     4441 aggcgacagc gggtggttca tcggttcgcc ggaagaagga gagttggccc acgaagcatt
     4501 cgacgcgata cgactgggcg acgtcatcgc cgcacgcgaa gatcttgcgg acatcctctc
     4561 gctgccgccc gggttcctgc tcgtgctcga ctccggcggc gtcgccgcca ttctcgatgc
     4621 cgcgggactc gacgtctggg cggttgcttt gatgaccgat gcggcgaaga atccatcggc
     4681 cgccgcgccc gctgggagct gagcgatggg cctgcaggaa agccgcggaa aactcgcccg
     4741 gtcgatgaag gacctgctgg cccaatgggc gctggtccgc tcgcaatgga acgacgtgaa
     4801 cgccgagcgt tttgaacgcg aggtgctgca catgctcgag atggatctgc gcaacgcatc
     4861 gggcgcgatg gaccagatgg gggcgctgct ggcgcagatc cgaagccagt gtgattgact
     4921 gccgcttgcg gcttagggtc tgttggttgg tctgtctatc cggcaaaaaa gaaacagacg
     4981 ctaagccgca agcggcaaat cgaccgcagt cccctgcgac ttcgcgcgtt ttgtatggta
     5041 aaactgccgc cgttcgctcg gtgcacccgt agagtaaatc cttgatgcca ccaccgttgc
     5101 cagacccgct cgacgaatcg ctcgtcacgc tcgaccgcct gccgcccacc gccgcccccg
     5161 cggcggccag cgcgggcgcc gcgccgcccg ccaccgcgga gaccgaccgc gacatccagc
     5221 gcagtgccct gcgcgatctg atcgcgctct ccaccgaatc cgcctccacc gaagccgaca
     5281 tcgagcgcga gcaccgcacc tcgctggaaa aggaaaagca gaccttcagc gatcgcgact
     5341 tccagatcaa cgaacagcac aagcagcacg tggccgacgc caaccagcag aaccaggacc
     5401 ggctgaatga gctggcatcg cagtaccagg ccgagcgcaa caagatcgtg tcagcggaca
     5461 aagcgtttcg gcagaagatc gaatccgaga agagcgcgat cgacgatcgt ctgaagcaga
     5521 agtacgacca ggcggtgtgg gaggccgaca gcgttaacga gatggcgagc caaaagatcg
     5581 ccgccgatta caaggcgacg accgaggcga tcaaggccgc cggtgatgcg ctcaacgcga
     5641 tggaggtgaa ggccgccgag ctggtgcagc tgtacggcct gccgctggcg actgacgcgg
     5701 tgaacgtcga tgacgatccg caaatcacca ccagcaccga ggtggcgttc gcgcgccata
     5761 aggaagcgat cgagcagcac ctgtcgcgcc tcggtcacat gccgctggcc agcctgttca
     5821 tcggcatccg gccgtacctg ttcctgctgc tcatcaccgc cgccgcggcc gccgccgtgc
     5881 agtttcacgc acaatcgacc gacccgcagt ggaaagcgct ggcgatcttc gccggcggta
     5941 cgttcgtcct ggcgctggcc atcggtttcg cgcttcgcct gctggccaat tcgaaggtgc
     6001 gcgcggtcta tcatccgctg cgacaggaac tggtgctggc ccgaagcgcc gcagagcagc
     6061 agcaggcgaa cgcggtagag aagcgcaagg ccgacaccgc cgccgcgacg aaagcgcggc
     6121 aggacgaagt gcaagccgcc aaagaagaag tcggcccgca ggccgccaag gcggcgcgaa
     6181 atcgcgacgc ggcgacggcc accgcgcagg ctgaattcgc caagcaggtc gcgcgcatcg
     6241 atcgcatccg cgacaccgcc aacgccgaga cggccgagcg cctgcgccgg atgatccacg
     6301 agatcgacca caagcaggag cgcgagctgc gcgacaacaa gacgcgcaac caaacccgcc
     6361 tcgtcgagat cgagcagcgc tatcagcaac gccgcgcgga actggaacag cattggagcg
     6421 aacggttgag gcgcattcag gctccgatcg atcgcgacgg cgacagcgtg ccggcatcgc
     6481 tcgactggaa cgacggcgct tggaaatcgt ggacaccgcc gaaaaaattc gcatcgcgcg
     6541 tgcgcttcgg cgagatgcaa gtggacctca agcagatcgc cgagaacgtc ccgcagcggc
     6601 tcacgctccc gccgacgttt tcactgccgg cgttgctcgc gttccccgcg caggcgtcgt
     6661 tgttgatcca caccgaccac gccggacgcg gtgaggcgat ccgcacgttg cagatgctga
     6721 tggcccgcct gctcaccaac cttccgccgg ggcgcgtgcg gtttaccatt ctcgatccgg
     6781 ttggtctcgg gcagaacttc gcggggttca tgcacctgtc ggattacgat gacgcgctgg
     6841 tcggtggccg catctggacg agccaggatc agatcgacca gcggctggcg aatctgaccg
     6901 atcacatgga aacggtcatc cagaaatatt tgcggaacga atttcagacg atcgacgaat
     6961 acaacgccca ggccggcgaa ctggccgagc cgtaccgctt cctggtcatc gcggacctgc
     7021 cggttggttt cacgtccgaa gcgttccgcc ggctcaacag tattgcatca accggtgctc
     7081 gctgcggtgt ctacacgttg atcgcccgcg acacgcgcgc cgcattgcct gctggagcgc
     7141 atcttgatga actggtcgcc cacagcgtga acctcgtgcg cgaaggcgac cgcttcgtgt
     7201 gggatgatga cgtctttcgt ccgctgccgc tgacgctcga cccgcctccg cgggaagatg
     7261 tattgttgac ggaaatcctg cacaaggtgg gcgccggcgc gaaggaagcg aagcgcgtcg
     7321 aggtgccgtt tgagaacatc gcgccgtcga atgaggcgat ctggtcgcgc agcacgacca
     7381 gcgagatcga cgtgccgatc ggccggctcg gggcgaaccg cctgcaatcg ctgaagctgg
     7441 gccgcggcgt cgctcaacac gtgctcatcg ccggcaaaac cggttcgggt aagtcgacgc
     7501 tgctgcacgc gatgatcacc aacgcggcga tgtggtattc gccggatgaa gtcgagctgt
     7561 acctgatcga cttcaaaaag ggcgtggagt tcaagaccta cgcgtcgaac ctgctgccgc
     7621 acgcgcgggc gatcgcggtg gaatccgatc gcgagttcgg cttgagcgtg cttcaacgtt
     7681 tggatgccga actctcccgc cgcggtgaac tgttccgaaa ggcggccgtg caggatctgg
     7741 cggcttatag caagacgccc aacgccgtcg tgatcccgcg cacgctgctg atgatcgacg
     7801 agttccagga attcttctcc gaagacgaca agctggcgca ggagtcagcg ctgctgatcg
     7861 accgtctcgt gcggcagggc cgcgcgtttg gcattcacgt gctgctcggt tcgcagacga
     7921 tcggcggggc aggcgggctg ccgcgcagca cgctggggca gatggccgtg cgcatcgcac
     7981 tgcagacgag cgaggcggat tcgcagttga tcctgggcga caacaactcg gccgcccggt
     8041 tgctcagccg gccgggcgaa gcgatctaca acgacgcggg tggactcgtt gaaggcaata
     8101 gcccgtttca ggtcgcgtgg ctgccggatg acaagcgcga caaatacctg cgcgatctgg
     8161 cgcggcgggc caaaccatat ttcgagcgtt tcggttatcc gatcgtcttc gaaggcaacg
     8221 ccccggcgga catccggcgc aacccacagc tgacggcagc gatggaagcc aagagctggt
     8281 cgcgcgtcgc cgccgctcgc gcatggctgg gtgaaccggt cgccatcaag gaaccgaccg
     8341 ccgtcacctt ccgccggcag gcgggggcga acatgatcat ggtcggccag caggacgagc
     8401 agtcgatggc gctcgcgtgc tcgtcgatcg tcagcctcgc cgcgcagcag gggtcggacc
     8461 aggcggtgtt ctacatcctc gacggcacgc ccgccgactc ggccctggcc ggcacgttcg
     8521 cccgcgtggg cgcggcattg ccgcacgacg tgaagctcgt cgaatggcgc gccacgcccg
     8581 aggcgatcga cgagatcgcc aaggaagtca cgcggcggca agacgtcgat caacccggcg
     8641 cgccgtcgat ctacgtcatc gtctacggcc tgcagcgcta tcgcgcgctc cggaaacagg
     8701 aagacgattt cggcagcttc tcgatggacg ccgacgagcc gaaaaaggcg aaccccggca
     8761 aacagttcgc cgagatccta cgcgacggcc cgccggtcgg cgtgcacgtc atcacctggg
     8821 ccgacacgct ggtggccgtc gatcgcacgc tcgaccgcgg ttcgatgcgc gagttcgaca
     8881 accgcgtgct gttccaaatg tccgcggccg attcgagcaa cctgatcgac tcgccggtcg
     8941 gcaacaaact cggcttcaac cgcgcgctgg tctacagcga agagcagggc gtgatggagc
     9001 gcttccgccc ttatgcgctc cccgacgggg cgtggctggc gacggtaagt tcgctgctga
     9061 atgccaggaa tacccaaccg gcgtgactcg cttgagcgag tcggcgtagc tgccgatccg
     9121 tctagtcctc gatctcaatc aaccacagcg aagatgcata acagctggcg acatcgattg
     9181 gcatcgcgag tggtactccc gtagcggtcg ccccgcaggg gcaggcgttt tccgttcgag
     9241 cgtcgtagca cgtgcccctt cggggcgatc gctacgcggg gagatttgat cttcgaacgg
     9301 cgactcggtg tgcgcaaaaa aaagagaggc cggtgctcgc cgttacaccg gcctccaaac
     9361 aggctgccca gccgaaaacg gccggagacc cacagccatt gtcacgctat cgggcactcc
     9421 gaaacgcaac tttaaaaatt caggcccgcg ggtcattccg atctgaaacg cgcgggacgc
     9481 ctccattg
//
LOCUS       DATIMH010000189         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4663520, whole genome shotgun sequence.
ACCESSION   DATIMH010000189 DATIMH010000000
VERSION     DATIMH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4663520"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1237)
                     /locus_tag="VLI90_03235"
     CDS             complement(80..1237)
                     /locus_tag="VLI90_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSV13242.1"
                     /translation="MDVVYLDNNATTKPAPEVVAAMLPYLTDLYGNASSVHRFGQRSR
                     QAVDEARGQVASLIHCSESEIVFTGVGTESINTAIRGILASRAPRRQIIISTVEHSAV
                     RELAKQLTREGVEVLEIPVDPRGSLDFDRLESVVGPDTALVSMMWANNETGVIFPVDR
                     IAALCRARGVPFHCDGTQAIGKVPVDLTAVPIDAMSFSAHKFHGPKGVGGLFVRRGVR
                     MRPFMIGGSQERNRRGGTENVPGIVGMGKAAELALASLPEMSSRVASLRDRFDQAVTG
                     NIDNAHVIGRTDSRVPNTTNIGFARLEAEAILLLLSEQGVCASAGAACSSGSLEPSPV
                     LQAMGIDPKIAHGAIRFSLSKYTTDAEIDRALAVLPSVIARLRAVLPVHAA"
     gene            1460..2440
                     /locus_tag="VLI90_03240"
     CDS             1460..2440
                     /locus_tag="VLI90_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSV13243.1"
                     /translation="MPSALTNFTECIARELSAVDDRFHAELSSDLRCVNTLVKHVSRF
                     RGKMLRPCLVLLSGKACAASELGDAHVTLATVVEMVHMATLVHDDVLDEAELRRKGAT
                     INHLRGNEAAVLLGDYLISHSYHLCSSLESQFASRLIARTTNQVCEGELLQIENRNNI
                     DLDDQTYLKIITLKTAVLTAACCTLGATFAGADERRVKALETYGLSLGVAFQIQDDIL
                     DIVGDTSTVGKTLGIDIEKGKMTLPMIHFLRTAPAEHRALLRSLLASRDADKVERIRN
                     LILPSKSIQHARDRAHELVDRAREAIADLPDSEAKRVLDAMAEFVVTRPM"
     gene            2715..3779
                     /gene="ligD"
                     /locus_tag="VLI90_03245"
     CDS             2715..3779
                     /gene="ligD"
                     /locus_tag="VLI90_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HSV13244.1"
                     /translation="MARASSTILARRVPTVRARRTSKIIDPVPATIAPMLAVGSDLPS
                     DPRNYTFEFKWDGVRAICYHDGSSRNFRIESRNQLDITRRYPELHALADALGKSPAIL
                     DGEIVSLDDAGRPSFAKLQHRMHLNGPSEIVRLSKSEPVFYVLFDVLYTRGRLVLNEP
                     YSARREMLQELTLAGAHWQITPAHVGEGKTMLDAARRNGLEGLVAKRLDSIYIPGKRS
                     PAWRKIKIIQRQEFVIGGYIPEKTGASNRIGALLTGYYDCDGKLRFAGKVGTGLSAAD
                     HAPLLKQLTRHPIAKSPFADPVPRGSIFNEPTIVAEIEYRRWPEGGSLQHASYKGLRM
                     DKNAREVVKEPNLAAKNNAA"
     gene            3879..>4447
                     /locus_tag="VLI90_03250"
     CDS             3879..>4447
                     /locus_tag="VLI90_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HSV13245.1"
                     /translation="MAESKSATPSGRPIWSGAISFGLVTIPVKLYTAVREKRIAFRSL
                     HDQDKVPLKQKLYCPADGKDVHPEHIVKGYEIEKDRFVVVRQEDLEAAHPKATKSIDI
                     QDFVELSEIDPLYFDRPYYIVPRPEGLKPYRLLVETMEKTGRVGIARVVMHTKEYLAA
                     LRPVEGALVMETMHFADEVVSSEGMGPGES"
BASE COUNT          844 a         1337 c         1483 g          783 t
ORIGIN      
        1 gtgggaacgt tcccgtgacg atcgcgcgat tgctgatcga cgtcttggcg atccttggcg
       61 cgatctgctt cgagcgctgt tacgccgcgt gtacgggcag cactgcccgc agccgcgcaa
      121 tgacgctggg cagcaccgcc agtgcgcgat cgatctctgc atcggtagtg tatttcgaca
      181 agctgaatcg gatcgcgccg tgggcgattt ttggatcgat ccccatcgcc tgtaagaccg
      241 gcgagggttc caagctgccg ctgctgcacg cggcgccggc ggacgcgcac acgccctgct
      301 cgctgagcag gagcaagatc gcctcggctt ccaagcgcgc gaagccaatg ttggtcgtgt
      361 tcggcacacg gctgtcggtg cggccgatca cgtgcgcgtt gtcgatgtta cctgtcacgg
      421 cttggtcaaa tcgatcgcgc agcgatgcga cgcgcgagga catttccggc agcgatgcca
      481 gcgccagctc cgccgccttg cccatgccga cgatgccggg aacgttttcg gtgccgccac
      541 ggcggttgcg ctcctggctg ccgccgatca tgaacggccg catgcgcacg ccgcggcgga
      601 cgaacagacc gccgacgccc ttgggcccgt ggaatttgtg ggccgagaaa ctcatcgcgt
      661 cgatcggcac ggcggtcaaa tcgaccggca ccttgccgat ggcctgcgtg ccatcgcagt
      721 gaaacggtac gccacgcgcg cggcacagtg cggcgatgcg atcgacgggg aagatgacgc
      781 cggtctcgtt gttggcccac atcatcgaca cgagggcggt gtcggggccg acgacggatt
      841 cgaggcggtc gaagtcgagc gagccgcgcg ggtcaaccgg gatctccagg acctcgacgc
      901 cttcgcgcgt cagctgcttg gccagctcgc gcacggcgga atgttcgacg gtcgagatga
      961 tgatctgccg gcgcggcgcg cgcgaggcga ggatgccgcg gatggcggtg ttgatggatt
     1021 cggtgccgac gccggtgaag acgatctcgc tttcactgca gtggataagc gacgcgactt
     1081 gaccgcgggc ttcgtcgacg gcttgtcggc tgcgctggcc gaagcggtga acgctggagg
     1141 cgttgccgta aaggtcagtg aggtacggca gcatcgcggc gacgacttcc ggggcgggtt
     1201 tggtggtggc gttgttgtcg aggtagacga cgtccattgc ggttgccaat cttaacgcgc
     1261 caaacgttga aattcccggt ggcgcggcgt gccggccgct tgcggcttaa cgtcttggtc
     1321 ttccacggag gaaacagacg ctatgccgca agcggcagtg gaaccgcacg ttttcaatct
     1381 tttcgaaact tccagcaccc attcgcgttg ggctggcgcc acttctatac tcccgcggcc
     1441 aatgaaggag cccatcggta tgccgtccgc cctcactaac ttcaccgagt gcatcgcgcg
     1501 cgaactcagc gccgtggacg atcgatttca tgccgaattg tccagcgatc tgcgctgcgt
     1561 caacacgctc gtcaagcacg tcagccgatt ccgcgggaag atgctgcggc cgtgtcttgt
     1621 gttgctgagc ggcaaggcgt gcgcggcgag tgaattgggc gatgcgcacg tgacgctggc
     1681 gaccgttgtc gagatggttc acatggccac gctggtgcac gatgacgtgc tggacgaagc
     1741 ggagcttcgc cgcaagggcg cgacgatcaa ccaccttcgc ggcaacgaag ccgcggtgct
     1801 gctgggggat tatctgatca gccacagtta tcacctgtgc tccagccttg aatcgcagtt
     1861 cgcgtctcgt ttgatcgcgc gcacgacgaa ccaggtatgc gaaggcgaat tgctgcagat
     1921 cgagaaccgc aacaacatcg atctggatga ccagacgtac ctgaagatca tcacgctgaa
     1981 gaccgcggtg ctgacggcgg cgtgctgcac gctgggggca acgttcgccg gcgcggacga
     2041 gcggcgcgtg aaggcgctgg agacgtacgg gctgtcgctg ggcgtcgcgt ttcaaattca
     2101 ggacgacatc ctcgacatcg tcggcgacac cagcacggtg ggcaaaacgc tcggcatcga
     2161 catcgagaag gggaagatga cgctgccgat gattcacttc ctgcgcaccg cgccggccga
     2221 gcatcgggcg ctgctgcgaa gcctgctggc cagccgggat gcggacaagg tcgagcgcat
     2281 ccgcaacctg atcctgccga gcaaatcgat ccagcacgcg cgcgatcgcg cgcacgagtt
     2341 ggtggatcgc gcgcgcgagg cgattgccga cctgccggat tccgaagcca agcgcgtgct
     2401 cgacgcgatg gcggagtttg tcgtgacacg tccgatgtag ggcccgacgc gcgcgagcga
     2461 gtccaagaat ctgccatcgc gctgagcgct gctgcacgtt gaggttacgc gaggcccgat
     2521 aaccaaatcg cacgtaagtg cgcgctggcg cgccacttgc atcaatcacg tcatagggcc
     2581 aatccggtgt gatgtcagga ggacggcgtg cagcccggca ggaaagctgg gcccgaggat
     2641 cggatcgccg ttggcgttaa ggatcaactg tttacatcta aatcgcagcc cccgctgcgg
     2701 cagcagaagc gcccatggca cgcgcgtcgt caaccatcct cgcgcgccgc gtacccaccg
     2761 tacgcgcgcg ccgaacgagc aagatcatcg atcccgtccc ggcgacgatt gcgccgatgc
     2821 tggcggtcgg gtctgacctt ccgtcggacc cgcgaaacta cacgtttgag ttcaagtggg
     2881 atggcgtgcg ggcgatctgt taccacgatg gttcatcgcg caacttccgc atcgagagcc
     2941 gcaatcagct ggacatcacg cggcgatatc cggagttgca cgcgctggcg gatgcgctgg
     3001 gaaaatcgcc ggcaatcctt gatggcgaga tcgtttcgct ggacgacgcc ggccggccga
     3061 gctttgcgaa gctgcagcat cgcatgcatc tgaatggacc gagcgagatc gttcggctgt
     3121 cgaaatccga gccggtgttc tacgtgctgt tcgacgtgct ctacacgcgc ggccggctgg
     3181 tgctgaacga accgtacagc gcgcggcgcg agatgctgca agagctgacg ctggccgggg
     3241 cgcattggca gatcacaccg gcgcatgttg gcgaagggaa gacaatgctc gatgcagcgc
     3301 ggcgcaacgg gctggaaggc ctggtcgcta agcggctgga cagcatctac atccccggca
     3361 agcgcagccc ggcgtggcgg aagatcaaaa tcatccagcg gcaggagttc gtcatcggcg
     3421 gatatatccc ggagaagacg ggggcatcca atcgtatcgg cgcgctgctg accggttact
     3481 acgactgcga tggcaagctc cgtttcgcag gcaaagtcgg cacaggatta tccgccgccg
     3541 atcatgcgcc gctgctgaag cagctgacgc ggcaccccat cgctaaaagt ccgttcgccg
     3601 atccagtgcc tcgcgggtcg atcttcaatg agccgacgat cgtcgccgag atcgaatatc
     3661 gccgctggcc cgaaggcggc tcgctgcagc acgcgagcta caagggcttg cgaatggata
     3721 agaacgcacg cgaggtggtg aaggaaccga atctggccgc gaagaataat gccgcgtagc
     3781 ggtagccccg aaggggcacg tgtttttccg tcgcgccaga aacacgtgcc ccttcggggc
     3841 taccgctacg cgggagaacg tgaacaacga ggcgcgttat ggcagaatca aaatccgcaa
     3901 ccccgtccgg ccggcccatc tggtccgggg cgatcagctt cgggctcgtc accatccccg
     3961 tgaagctcta caccgcggtg cgcgaaaagc gcatcgcgtt tcgcagtttg cacgaccagg
     4021 acaaggtgcc gctgaagcag aagctgtatt gccccgccga cgggaaggat gtgcatcccg
     4081 agcacatcgt gaaggggtat gagatcgaga aggaccgctt cgtcgtggtg cggcaggaag
     4141 atctggaggc ggctcatccg aaggcgacga agtcgatcga tatccaggat ttcgtcgagc
     4201 tgtcggagat cgatcccctt tacttcgatc gcccctatta catcgtcccg cggccggagg
     4261 gattgaagcc ttatcgactg ctggtcgaga cgatggaaaa gaccggccgc gtcggcatcg
     4321 cgcgggtggt gatgcacacg aaggaatatc tggcggcgct gcgaccggtc gagggcgcgc
     4381 tggtgatgga gacgatgcac ttcgccgatg aagtcgtgtc cagcgaaggc atggggccgg
     4441 gcgaatc
//
LOCUS       DATIMH010000190         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_42404, whole genome shotgun sequence.
ACCESSION   DATIMH010000190 DATIMH010000000
VERSION     DATIMH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42404"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2481
                     /locus_tag="VLI90_03255"
     CDS             <1..2481
                     /locus_tag="VLI90_03255"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HSV13246.1"
                     /translation="PVAAPPVTPSTPPPTQPSTASAPDVAVRKIIRNGTIEFEVRSFD
                     DTYATVATVVSENSGFVSSTSSDKLANGKVRGSIVVRVPPERLDRFLLQLRSLGDLKS
                     QQIAANDVTKQYTDIASELRGLRTMEGRLLDLIKTGKGEVKDLVEAEKQLGEYRIRIE
                     KLEGEISYYNNLVGMATITITAYEKDIQKPTAAAEQESVILNVETEEVETKYQAARKV
                     IEEAKGRIVESQLKNTDAEHIAATIVADVPPDKADFVAAQLKQLGKVSSYNRDRRQTT
                     TGGPGAPPPGVQVEQKDTRFSIALYNLANLAPRETTVLRVAVRDVQDTYRKTLSAVRK
                     ETGAPGAAPAEGGDNAANNAADKAATRAAGRVLSSNLTGQQADQMAATIVAEVRADQA
                     DAVLELIRSSGEVLASALATNQDNASSTSAKRGIQLTLVNFSTVAPKEVDTLNIAVPD
                     VDEAYKKLLVLIRTDVPPPAPGQAPANPLLPSTTGRVLASNVNGQQPEQMVADIRAEI
                     RSDQAQRVLDAVRGSGEILNSTSAETPEANNSTLAKRGLQVRLVNVAAIGAREAQALR
                     LVAADVPEAYTRLLSSLQDMQTAGNARVISSQLNQNDARNVTATMTFDAQRKALPSVD
                     KAFKDAGVDFLSRNIVRSTDTSGTLDTKVRFQIDALVSADSLEPRRKTVLAVEVDDPE
                     KAMEIMRSGIAGTPAREVDFNMASDKSGRVTGTIVIEAPVSNIPAILDRAHHLGDEKV
                     NQVVKNQQVSDTRFARERVELTLSSRAPIVSSDKGLGSTIRAALSAAAGALLYSMYLV
                     VTGVLFVLPFALLIWVGMVVFRRRKAAA"
BASE COUNT          581 a          887 c          928 g          468 t
ORIGIN      
        1 cccgtcgccg ctccccccgt cacgccgtca accccgccgc ccacgcagcc ctccaccgca
       61 tccgcccccg atgtcgccgt gcgaaaaatc atccgcaacg gcaccatcga atttgaagtc
      121 cgctcctttg atgacaccta cgccaccgtc gccaccgtcg tctctgaaaa cagcggcttc
      181 gtctcctcca ccagctccga caagctcgcc aacggcaagg tccgcggcag catcgtcgtg
      241 cgcgtcccgc cggaacggct cgatcggttc ctgctgcaac tgcgctcgct gggcgatctc
      301 aagagccagc agattgccgc caatgatgtc accaagcagt acacggatat cgcctccgag
      361 ctccgcggcc tgcgcacgat ggaggggcgg ctgctcgatc tgatcaagac cggcaagggc
      421 gaagtgaagg acctggtcga ggcggaaaag cagctcggcg agtaccgcat ccgcatcgaa
      481 aagctcgagg gcgagatcag ttattacaac aacctcgtgg gcatggcgac gatcacgatc
      541 acggcctatg aaaaggatat ccagaagccg acggccgcgg cggagcagga aagcgtgatc
      601 ctgaacgtcg agaccgaaga ggtggagacg aaataccagg cggcgcggaa ggtgattgaa
      661 gaggcgaagg gtcggatcgt cgagagccag ttgaagaata cggatgccga gcacatcgcg
      721 gcgacgatcg tggcggacgt gccgccggac aaggcggatt ttgtcgcggc acagctcaag
      781 cagctgggaa aagtctccag ctacaaccgc gatcggcggc agacgacgac gggcgggccc
      841 ggcgcgccgc cgccgggcgt gcaggtggaa cagaaggaca cgcggttcag cattgcactt
      901 tataacctgg cgaatctggc cccgcgcgag acgacggtgc tgcgcgtggc ggtgcgcgac
      961 gtgcaggata cgtatcgcaa aacgctttct gccgtgcgca aggagacggg cgctccgggg
     1021 gcggcgccgg ccgaaggcgg ggacaatgcg gccaataatg ccgccgacaa ggccgcgaca
     1081 cgagcggcgg gccgcgtgtt gtcgtcaaat ctcacgggcc agcaggcgga ccagatggcg
     1141 gccacgatcg ttgcggaagt tcgcgccgac caggcggacg cggtgctgga gctcattcgc
     1201 agctcgggcg aggtgctcgc cagcgccctg gcgacgaatc aggacaacgc gtcgtcgacg
     1261 tccgccaagc gcggcattca actgacactg gtgaattttt caaccgtcgc ccccaaggaa
     1321 gtggacacgc tgaacattgc cgtgcccgat gtggatgagg cgtataagaa gctacttgtg
     1381 ttgatccgta cagatgttcc cccgccggcc cccggccagg ctccggccaa tccactgctg
     1441 ccttcgacga cggggcgcgt tctcgcgagc aacgtcaacg gccagcagcc cgagcagatg
     1501 gtggcggaca ttcgcgcgga gattcgttcg gatcaggcgc agcgcgtgct ggatgccgtg
     1561 cgcggctcgg gggaaatctt gaacagcacc tccgccgaaa cacccgaggc gaacaattcc
     1621 acgctggcca agcgcgggct gcaggtgcgg ctggtgaatg tggcggcgat cggggcgcgg
     1681 gaggcgcagg cgctgcggct ggttgccgcc gacgtgcccg aggcatacac ccggctgctc
     1741 agttcgctgc aggatatgca aaccgccggc aacgcgcggg tcatctcctc gcagctcaat
     1801 cagaatgacg cgcgaaacgt caccgccacg atgacgtttg acgcgcagcg caaagccctg
     1861 ccctccgtcg acaaggcctt caaagatgcc ggcgtcgatt tcctctcgcg gaacatcgtg
     1921 cgctccaccg acacctccgg cacgctggac acgaaggtgc gcttccagat cgacgccctc
     1981 gtctccgccg actcgctcga gccgcggcgg aagacggtgc tggccgttga agtcgacgat
     2041 ccggaaaaag cgatggagat catgcgcagc ggcatcgcag gcacgccggc acgagaggtc
     2101 gatttcaaca tggccagcga caagagcgga cgcgtcacgg gcacgatcgt gatcgaagcg
     2161 ccggtctcaa acatcccggc aattctcgac cgggcgcatc acctcggcga tgaaaaggtc
     2221 aatcaggtgg tgaagaatca gcaggtgtcc gacacgcgct tcgcgcgcga acgcgtggaa
     2281 ctcacgctgt cgagccgggc gcccattgtg tcgtcggaca aggggctggg ctcgacgata
     2341 cgcgcggcac tctcggcggc ggcgggggcg ctgttgtaca gcatgtatct ggtggtgacg
     2401 ggcgtgctgt tcgtgctgcc attcgcgctg ctgatctggg tggggatggt ggtgtttcgc
     2461 cggcggaagg cggcggcgtg aggtgttgct caagagcgtt ttcggaagag cttgcgaaag
     2521 ttccgccggc cgtggatgac tcgaacaatg gtgacagagg cgtcgtcgta ccggtatgca
     2581 attacgtatg aatatagatt ccaaaaatgg aaacccgctt cttttacatc cgcgcgtcta
     2641 tgccccttgc caggcatgac ggttaaatcg cggagcgtcg cgtcaaattc ctcccccact
     2701 cggttcgccg ttgacacgct gtcctgtgcg ataaaatgcc agatatcaag aagatccagc
     2761 cgggcttgcg gcttgaagcg caattctctc atccgcgatt accctggcgc tgcgccatgc
     2821 gaagatcgcg caattcctta aggacggtct cgccatttag ggca
//
LOCUS       DATIMH010000191         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4083840, whole genome shotgun sequence.
ACCESSION   DATIMH010000191 DATIMH010000000
VERSION     DATIMH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4083840"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VLI90_03260"
     CDS             complement(<1..553)
                     /locus_tag="VLI90_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HSV13247.1"
                     /translation="MRERGQHRKGSRDSARHATRGNDGITIISGGQTGADRAALDVAL
                     ALHIPCGGWCPSDRAAEDGTIPAKYPLTPLPGRGGYRQRTRRNVHDSDGTVIFSYGPL
                     TGGSKATAGDCRHFRKPCLMIDANVDGPMDAALSLAVFILRHHVRRLNVAGPRASGQP
                     GIYEFVRGALMAMLKKPPRRRRRG"
     gene            623..1537
                     /locus_tag="VLI90_03265"
     CDS             623..1537
                     /locus_tag="VLI90_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HSV13248.1"
                     /translation="MPQNKVILAGGSGFLGQALVPFLLKRFESIIVLTRNAARSDGPV
                     RYVSWDAKSQGPWTAEIDGATAIVNLVGRTVDCRKTPANKREILESRVHSVRALAAAC
                     NAVANPPNVWVQAGTAHIYGDTADEILDESSPLGSGFAPDVGRAWEAAFDNADLPNCR
                     HIMLRTSFVLGRHGGALATLAKLTRCFLGGTVATGRQYISWIHVDDFCRIVMRAIENP
                     TMEGVYVVTAPNPVTNRDFMAALRRTLHRPWSPPAPAACVRLASLLMRTDPELALLGR
                     RCIPTRLMREVFEFQFPKLNDALRELLL"
     gene            1613..1849
                     /locus_tag="VLI90_03270"
     CDS             1613..1849
                     /locus_tag="VLI90_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13249.1"
                     /translation="MSRLQKDDGAGGAAPRTIDDIAREQGINAPQELDRMIGSAANLW
                     ENDEEFESFVQGIHTRRREGLAAETRKAAGESQA"
     gene            complement(2273..>2863)
                     /locus_tag="VLI90_03275"
     CDS             complement(2273..>2863)
                     /locus_tag="VLI90_03275"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HSV13250.1"
                     /translation="YSLADWYPAFHVTAITHRKGPVYPTTIVGKPPMEDYYLGKATER
                     VFLPLLRMMVPDIIDYSLPISGVFHNCAFIKIRKEYPYQARRVMHAIWGAGQMSFTKF
                     IIVVDEQVNVHDEQDVLFHLFSNCDPARDTEIVHGPVDILDHASPELGAGSKIGFDAT
                     VKLPAEGKVRAWPKELEFDAATRALVERRWREYGIG"
BASE COUNT          547 a          995 c          814 g          507 t
ORIGIN      
        1 cccctcgcct tcgccgccgc gggggcttct tcaacatcgc catcaacgcc ccccgcacaa
       61 actcataaat ccccggctgc ccgctggcgc gcgggccggc gacgttcaac cgccgcacgt
      121 gatgccgcag gataaaaacc gccaacgaca acgccgcatc catcggccca tcgacattcg
      181 catcaatcat caggcacggc ttacgaaaat gccggcaatc acccgccgtc gccttcgacc
      241 cgccggtgag cggaccataa ctaaagatca cggtcccatc gctgtcgtgc acattccgcc
      301 gcgtgcgctg ccgatacccg ccgcggcctg ggagcggcgt caacggatac ttcgccggga
      361 tcgtcccgtc ttccgccgcg cgatccgatg gacaccaccc gccgcacggg atgtgaagcg
      421 ccagcgccac atcgagcgcc gcccgatccg cgccggtctg cccgccggag atgattgtaa
      481 tcccgtcgtt cccccgcgtg gcgtgacgcg ccgaatcgcg gctgcccttg cgatgctgac
      541 cccgttcccg cacgaccata ttcaactttc aaaacccgcc gccgcgccgc ccgcgctaaa
      601 gatcacgcga taaactttat cgatgcccca gaacaaggtc atcctcgccg gcggttccgg
      661 cttcctcggc caggcgctcg tcccgttcct gctcaaacga ttcgagtcaa tcatcgtcct
      721 cacgcgcaac gccgcgcgca gcgacggtcc cgtgcgctac gtgtcatggg acgcaaaatc
      781 gcagggacca tggaccgcag aaatagacgg agccaccgcg attgtaaacc tcgtcggccg
      841 gaccgtggac tgccgcaaga cgccggcgaa caagcgcgag attctcgagt cgcgcgtgca
      901 ctccgtccgc gccctcgccg ccgcgtgcaa cgccgtcgcc aacccgccga acgtgtgggt
      961 tcaagccggc accgcccaca tctacggcga caccgccgat gaaattctcg acgagtcatc
     1021 cccgctcggc agcggcttcg cccccgacgt cggccgcgcc tgggaagcgg cgttcgacaa
     1081 tgccgacctg cccaactgcc gccacatcat gctgcgcacc agcttcgtcc tcggccgcca
     1141 cggcggcgcg ctcgcgacgc tggccaagct cacccgctgc ttcctcggcg gcaccgtcgc
     1201 caccggccgg caatacatca gctggatcca cgtcgacgac ttctgccgca tcgtcatgcg
     1261 cgcgatcgag aacccaacta tggaaggcgt ctacgtcgtc accgccccaa accccgtcac
     1321 gaaccgcgac ttcatggccg ccttacgccg cacgctacat cgcccctggt ccccacccgc
     1381 cccggccgcg tgcgttcgcc tggcatcgct cctgatgcgc acggaccccg aactcgccct
     1441 cctcggccgc cgctgcattc caacccggct gatgcgagag gtgtttgagt ttcaattccc
     1501 aaaactgaat gacgccctac gcgagctttt attgtgagaa tgcgcggatg ctccagccgg
     1561 tttacgcgaa gctatatata aatgagcctc gtctaaatcg ggaggctatc aaatgtcacg
     1621 actgcagaaa gacgacggtg cgggcggtgc ggcaccgcgc acgatcgatg acatcgcgcg
     1681 cgaacaggga atcaatgcgc cgcaggaact ggaccggatg attggctccg ccgccaatct
     1741 ttgggagaac gacgaagagt tcgagtcgtt cgtgcaaggt atccatacca gacgaaggga
     1801 ggggctcgcg gcggaaacgc gcaaggctgc cggggaatcc caagcgtgaa tacctacctc
     1861 ctcgatacga acgtcgtttc atacttcttt cgcggcgaca acctcggatt gccatatcgt
     1921 tcaatggtgc aaggtgggcg cctcgccatc tcgtttgtga cagtgggcga gttgtacgaa
     1981 gtcgcatatc gcgcaggttg gggcgcggct aagcttcgaa cattgggaac cgaaatcacg
     2041 aaatacgtcg tcgtccactc ctccgaatcg caatgtcgca tttgggctga ggtccggacg
     2101 gcacgacgac atcaaccgat cgccggcgac gatgcatgga ttgcggcagc tgccttagag
     2161 ttgtctttac ccctggtgac ccacaatcgc gcggatttca atgggattcc gggggctgtc
     2221 agtcatctcc gcctctccct gagaaggata aaatccttaa gccgggggaa gttcaaccga
     2281 tcccatattc ccgccaccgc cgctccacca gcgctcgcgt cgcggcgtca aattccaact
     2341 ccttcggcca cgcgcgaact ttcccctccg ccggcagctt caccgtggcg tcaaagccga
     2401 tcttgctgcc ggcgccgagt tccgggctcg cgtgatccag gatatcgacg gggccgtgga
     2461 cgatttccgt gtcgcgggcc gggtcgcagt tgctgaacag gtggaacagc acgtcctgtt
     2521 catcgtgcac gttcacctgc tcgtccacca cgatgatgaa cttcgtgaag ctcatctgcc
     2581 cggcgcccca gatcgcgtgc atcacgcgcc gggcctggta cgggtattcc ttgcggatct
     2641 taataaaagc gcaattgtgg aagacgccac tgatcggcag gctgtagtcg atgatgtccg
     2701 gcaccatcat ccgcagcagc ggcaagaaga cgcgctccgt cgccttgccc aggtaatagt
     2761 cctccatggg cggcttgccg acgatcgtcg tggggtagac ggggcccttg cggtgggtga
     2821 tggccgtgac gtggaacgcc gggtaccagt cggccaggga gta
//
LOCUS       DATIMH010000192         5293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3795523, whole genome shotgun sequence.
ACCESSION   DATIMH010000192 DATIMH010000000
VERSION     DATIMH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3795523"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VLI90_03280"
     CDS             <1..985
                     /locus_tag="VLI90_03280"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HSV13251.1"
                     /translation="GGMILLHNENATPEMDVFAEQLAKQAYPEFPLVDLPPDHLLYSV
                     VYPMKKKTPLKGVSNGHRLLMVYSPTDITKGWLIRQGRGTNEQAQLGLNLFVYAAGKT
                     NFHGRLITPYIPPPLFAALGSIPIARIKYTGGNWDPEPFAWQRFPRWFQEQTSIALSV
                     TPQAIEELNFPAVPVAVLTGDSAVDFDKMNLQPLHDYVAAGGLVVIDSVAGNQPFIQS
                     VRDKLLPKAFADGQIQQLDTSHPLLAGTGDGMDPLPKAQLRQFAIEQLGHNLPALDIV
                     TLGKGAVIISDIDLTCGLLDTSTWGITGYEPNYCNSLVKNVILWASERYHL"
     gene            1271..3997
                     /locus_tag="VLI90_03285"
     CDS             1271..3997
                     /locus_tag="VLI90_03285"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HSV13252.1"
                     /translation="MILCPACGFRFVVPGISRMTPLAAAPDAATGPSPATPATSPPAA
                     SHRGLQIIVGICLMAIIAAAAFFIHNATATREASPPPAIAPAAPAPATPRRVVATKPA
                     TTQATPQMSQLLQHAATSQAIVEVPPPLPAQPPTEPASQPATTAATEPAPAPSTSPAP
                     PLPRIHPVRPTVDTASVDLDDQIGQSIRRGVDYLLNRMKTGRLQKTKDEEIDNDTLAG
                     LDALVVYALLHAGEATNDERLGPHTTIVDDMLKVLKALPADHGPATYARALRAAALGV
                     YNRGEDRTSLQADATWLLKANIGGAYSYELPRPGMRGTRGVGENEWDNSNSQYGALGV
                     WSADEAGVSVPQSYWTEVEQHWLGCQLPSGEWAYAGPSEFGRLSMTVAGITTLFVTQD
                     QLSGSHAANVSVLGQPPFTRALARGLQWLESGDNSVTLPLHWRTYNLYGLERAALASG
                     FKYFGTHDWYRELAKDALLSQRPDGSWFTADTNTDEAVDTAFTLLFLSRGRHPIFMNK
                     LRYDGFWANRPRDAANLTRFATHELERPLNWQVVNLSGDWIDWMDSPVLYMAGHQPPK
                     LEQSDYAKLKAYAENGGLIFTHADGDSADFTRFAQDLARHLFPQYALQDLPPTHEIYT
                     TLYKLKAGPRLQGVSNGSRLLLLHSPTDLNKAWQLRDWKPRQTPFEMGLNTFIYAAGT
                     VNLRNKLHTPYVPEPGIAPLATVNLPRLRYDGNWDPEPAAWSRFGRQFLGATSIDVRP
                     TPIDVADLRVNSGQLAHLTGTSEVHFSDDQIAALRNYVNAGGVLLIDACGGSTAFAQS
                     IRAELLPRAFPDGQLKRLRADHPILSGDGGADIEGLLRITPHLRPHATELLGITGVPI
                     ETLTAGRGCVIFTGVDLTTGLLGTNTYSVIGCDPETATPLCRNILLWALQRD"
     gene            complement(4541..4909)
                     /locus_tag="VLI90_03290"
     CDS             complement(4541..4909)
                     /locus_tag="VLI90_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13253.1"
                     /translation="MAPIVIGSCAAVMLLSNARTLGGGFKRALGSGGGAGGARARRPS
                     PRPNMATDGRSLAGLRNAILGSGKPQIASVFGPPHSAAIRGGVGDTWYYPLQPREKLA
                     MAISFDDGTAREVEFFHAPD"
     gene            complement(5019..>5293)
                     /locus_tag="VLI90_03295"
     CDS             complement(5019..>5293)
                     /locus_tag="VLI90_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13254.1"
                     /translation="AAVAVLREMNRVARRGVVVADLIRRRRAYAWISLFTLLANPMVR
                     HDARVSVAQAFSRREILALRDRAGLSFADYAEHFGHRFVLAGDKPQ"
BASE COUNT         1010 a         1801 c         1611 g          871 t
ORIGIN      
        1 cggcggaatg atcctgctgc acaacgaaaa cgcgacgccg gagatggacg tctttgccga
       61 gcagctggcc aagcaggcgt atccggaatt cccgctggtg gacctgccgc cggatcatct
      121 gctttactcc gtcgtttatc cgatgaagaa aaagacgccg ctcaaaggcg tgagcaacgg
      181 gcatcggctg ctgatggttt attcgccgac cgacatcacc aagggctggc tgatccgcca
      241 gggccgcggc accaacgagc aggcgcagct gggcctcaac ctgttcgtgt acgcggcggg
      301 caagacgaac tttcacgggc ggttgatcac gccgtacatt cccccaccac tgttcgcggc
      361 gctgggcagc atcccgatcg cgcgcatcaa atacaccggc ggcaactggg accccgagcc
      421 gtttgcatgg cagcggtttc cgcgttggtt ccaggagcag acgagcatcg cgctgtcggt
      481 gacgccgcag gcgatcgagg agctgaattt ccctgcggtg cccgtcgcgg ttttgacggg
      541 cgactcggcc gtggacttcg acaagatgaa cctgcagccg ctgcacgact acgtcgccgc
      601 gggcgggttg gtggtgatcg attcggtcgc gggcaatcag ccgttcattc aatccgtgcg
      661 cgataagctg ctgccgaagg ctttcgccga tggacagatc cagcaactgg acacgtcgca
      721 cccgctgctg gccggcacgg gcgacggcat ggacccgctg ccgaaagcgc aactgcggca
      781 atttgcgatc gagcagctcg ggcataatct acccgcgctg gacatcgtga cgctcggcaa
      841 aggcgcggtg atcatcagcg acatcgacct gacctgcggg ctgctggaca ccagcacgtg
      901 gggcatcacc ggctatgagc cgaactactg caactcgctg gtgaagaacg tgatcctatg
      961 ggcgtcggag cgatatcatc tgtgacgtcg catgggcatc ttgcccgtgt ttcgtggtac
     1021 accgcgaatg ccgacggtac accgttgacg aagcaatcgc gcaagcgacc gtgcggcagt
     1081 cgagtcgctg aggcgattgc tgtgtcgatc gcgtaccatc gacgtgcgcg tgtacacgca
     1141 ccacgggcca gaggcaccgt gtcgctttag taaatcacca cggtaggcgc acctgcaatc
     1201 catgcccaat cgcattaaat gcccccgctg cggccgagcc cagagcgtcg atgacgcgga
     1261 tgctgggcat ttgatcttgt gccccgcgtg tgggtttcga tttgtcgtgc cgggcatctc
     1321 gcgcatgacg ccgttggcgg ctgcgcctga cgcggcaacc ggtccatcgc cagcaacacc
     1381 cgctacatcg ccgccggctg catcgcatcg cgggctgcag atcatcgtcg gcatctgtct
     1441 aatggcgatc atcgccgccg cggcattctt cattcacaac gccaccgcaa cacgcgaagc
     1501 atcgccgccg ccggcgatcg cgccagccgc gccagcacca gcgacaccgc gacgcgtggt
     1561 cgcgacgaag cccgcgacga cgcaggcgac gccgcagatg tcgcagctgc ttcaacatgc
     1621 cgcgacgagc caagcgatcg tcgaagtacc accaccgctg ccggcgcagc ccccgaccga
     1681 gccggcatcg caacccgcga caaccgcggc gacggagccc gcgcccgcgc catccacatc
     1741 acccgcgcct ccgctgccgc gcatccatcc ggttcgaccc accgtcgata ccgcctcggt
     1801 cgatctcgac gatcagatcg gccagtccat ccgccgcggc gtggactatc tgctgaaccg
     1861 catgaagacc ggccgactac agaaaacgaa ggatgaggag atcgacaacg acacgctcgc
     1921 cggtctcgat gcgctggtcg tgtacgccct gctgcacgcg ggcgaggcga cgaacgacga
     1981 gcggctcggg ccgcacacga cgatcgtcga cgacatgctc aaggtgctca aagcgctgcc
     2041 ggccgatcac ggtccggcga cgtacgcacg cgcgctgcgc gccgcggcgc ttggcgttta
     2101 caaccgcggt gaagatcgca catcgctgca agccgatgcg acgtggctgt tgaaggcgaa
     2161 catcggcggc gcgtacagct atgagttgcc tcgacccggc atgcgcggca cacgcggcgt
     2221 cggtgagaac gagtgggaca attccaattc ccaatacggc gcgctcggcg tctggtccgc
     2281 cgacgaggcc ggcgtcagcg tgccgcagtc gtactggacg gaagtcgagc agcattggct
     2341 cggctgccaa ttgcccagcg gcgaatgggc gtacgcgggg ccgtcggaat tcgggcgatt
     2401 gtccatgacc gtcgcgggca tcacgacgtt gttcgttaca caggatcaac tttcaggatc
     2461 acatgcggcg aacgtcagcg tgctgggtca accgccgttc acccgcgcgc tggcgcgcgg
     2521 gctgcagtgg ctcgagtccg gcgataattc ggtcacgctg ccgctgcact ggcgaacgta
     2581 caacctgtac gggctggagc gggccgcgct ggccagcggg ttcaaatatt tcggcacgca
     2641 cgattggtac cgcgaattgg ccaaggacgc gctgctgtcg cagcggccgg acggttcctg
     2701 gttcaccgcc gacaccaaca ccgacgaagc ggtggacacc gcgttcacgc tgctgttcct
     2761 ctcgcgcggg cggcatccga tcttcatgaa caagctgcgc tacgacggct tctgggccaa
     2821 ccgcccgcgc gatgccgcca acctcacgcg cttcgccacg cacgaactgg agcggccgct
     2881 caactggcaa gtcgtgaacc tcagcggcga ttggatcgac tggatggatt cgccggtcct
     2941 gtacatggcg ggtcatcaac cgccgaagtt ggagcagagc gattatgcga agctcaaggc
     3001 atacgccgag aacggcggac taatctttac gcatgccgac ggcgacagcg cggacttcac
     3061 gcgcttcgcg caggacctgg ccaggcatct gttcccgcaa tacgcgctgc aggacctgcc
     3121 gccgacgcac gagatttaca cgacgctcta caagttgaaa gccggcccgc ggctgcaagg
     3181 cgtcagcaac ggctcgcgcc tgctgctgct gcattcgccg acggacctga acaaggcgtg
     3241 gcaactgcgc gactggaagc cgcggcagac gccgttcgag atgggcctga acacgttcat
     3301 ctacgccgcc ggcacggtga atttgcggaa caagctgcac acgccgtacg tgccggagcc
     3361 cggcatcgcg ccgctggcga cggtgaactt gccgcggctg cgatacgatg gcaactggga
     3421 ccccgagccc gccgcatggt cgcgcttcgg ccggcagttt ctcggcgcga ccagcatcga
     3481 cgtccgaccg acgccgatcg acgtcgccga ccttcgcgtc aattccggcc agctcgcgca
     3541 cctgaccggc acgagcgaag tgcatttctc cgacgaccag atcgccgccc tccgcaacta
     3601 cgtcaacgcc ggcggcgtgc tgctgatcga cgcctgcggc ggatcgaccg cctttgccca
     3661 aagcatccgc gccgaactgc tgccgcgcgc gttccccgac ggacaactga agcgattgcg
     3721 cgccgatcat cccatcctca gcggcgacgg cggcgccgac attgaaggcc tccttcgcat
     3781 caccccgcac ctgcgcccgc atgcaacgga actgctcggc atcaccggtg tgccgatcga
     3841 aaccctcacc gccggtcgtg gctgcgtcat cttcaccggc gtcgatctaa ccaccggtct
     3901 gctcggcacc aacacgtaca gcgtgatcgg ctgcgacccc gaaacggcga cgccgctgtg
     3961 ccggaacatc ctgctttggg cgctgcagcg ggactaagag ccttggcgcg caagaaaaaa
     4021 atcctgcgat gttgcgcgcg catcaggacg cgtaacatat tcatgcacag ctacttatat
     4081 ttatgagtcc ggcgaagaaa accacaagcg agttgcgcaa catgcgattt tatacccata
     4141 tttcccaaac ttcccatctt catccagcgc cgggttctga acgtactccg accgagcgag
     4201 attctgcggc gcatcgatca gaaagcatcc gggtcgtcga atacctccga acccggcgtg
     4261 gagggagacc agccattctt gccgccggca aagtccgggg aaatgtaacc gccgagccgc
     4321 cgagcgcgcc gaggcagacg ccgagaagag gctcagagaa gagttttcaa ccacagattt
     4381 cgcagcttac acagattgaa aagccagttt ctgaatctgt gtaatttgtg aaatctgtgg
     4441 tttaaccctc ttctgctctt gtcttctcgg catccgcctc ggcgcccgcg gcgcccgcgg
     4501 cggcctcggc ggttgcattt cacgaacggc ccacgacaga tcaatccggc gcgtgaaaga
     4561 actccacctc gcgagccgtc ccatcatcga acgaaatcgc catcgcgagt ttctcgcgtg
     4621 gctgcagcgg gtagtaccag gtatccccca ccccgccgcg aatcgccgcc gaatgcggcg
     4681 ggccgaagac cgacgcgatc tgcggtttgc cgctgccgag gatcgcgttg cgcaaccccg
     4741 ccagcgagcg gccatccgtc gccatattcg gtcgcggact cgggcgtcgc gcgcgagcgc
     4801 cgcccgcccc gccgccgctg cccagggctc gtttgaatcc gccgcccagc gttcgcgcgt
     4861 tcgacagcag catcaccgcg gcacacgatc cgattacgat cggcgccacc agaagggctc
     4921 gaagccatgc ggcttcccgg gatggtgagt taccgggcga cagcatcatg ctgaaatcta
     4981 ccaaatccgc gcccgcggca aaacccgcgt tcgccgcttc actgcggctt gtccccggct
     5041 aacacgaacc gatgaccgaa atgctcggca taatcggcaa agcttaaccc cgcccggtcg
     5101 cgcagcgcca ggatttcgcg ccggctgaac gcctgcgcca cgctcacccg ggcatcgtgc
     5161 cggaccatcg ggttggccag caacgtgaac aggctgatcc acgcatacgc ccgccgccgg
     5221 cggattagat ctgcgacgac cacgccacgc cgggccacgc ggttcatctc gcgcagcacc
     5281 gccaccgcgg cgt
//
LOCUS       DATIMH010000193         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1485960, whole genome shotgun sequence.
ACCESSION   DATIMH010000193 DATIMH010000000
VERSION     DATIMH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485960"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..723)
                     /locus_tag="VLI90_03300"
     CDS             complement(250..723)
                     /locus_tag="VLI90_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSV13255.1"
                     /translation="MKRSQSQFVSSRWLSACALIAMVPALAMSSKSPTSARPSASRTV
                     WDSVYSVPQAARGESTYAKTCARCHGASLTGGDESPPLTGGAFLGNWNGLPLSDLQTR
                     IKTTMPTDSVGIYDRQLITDVMAFLLKVNGFPAGQAALPADGDALKEITLQATKP"
     gene            complement(731..>2600)
                     /locus_tag="VLI90_03305"
     CDS             complement(731..>2600)
                     /locus_tag="VLI90_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSV13256.1"
                     /translation="LDQINAANFSTLEVAWRFKTDSLGPRPEFKLEGTPLMVRGTLYT
                     TGGTGRAVVALDAVTGAQKWVHREDEGERGAAAPRPLSGRGLAYWTDGRDERIIYVTP
                     GYRLMALDAKTGEVIPSFGTNGVVDLKTDVDQTIFPDLITGEIGYQGAPVVGRDVVVI
                     GAAFKEGSAPKSYKNNKGYIRAFDVRTGKRLWTFHTIPKKGESGYETWLNGSAEDAGN
                     TGVWTQVTIDEDLGLVYLPVESPTGDYYGGHRPGNDLYGESLVCLDLRTGAKKWHYQI
                     VHHPIWDFDLASAPILADANVDGRPVKTVAVPTKQGILYVFDRVTGKPVWPFEEKPVE
                     KGDVPGEWYSPTQPIPTKPRAYAKNGISTDDLVDFTPELHAQALAIASRFKLGPIFTP
                     PVVSKAEGPIGTLARGPTNGGSNWPGGSYDPETHMVYLASSNAAPVVLGLVQPSKERS
                     DMSWIAGIASAPGATGGGGGRGGAALSIQGLPIFKPPYGTISAINLDKGEIAWQVPHG
                     ETPDAVRNSDALKGMTIPRTGQPGFIVGTLVTKTLVIAGDPQVTTLPTRPRGAMLRAY
                     DKATGAEVGSVLLPAPISGSPMTYSVNGKQYIVTAVSGGPYSGEYIALRLPDAAPK"
BASE COUNT          462 a          795 c          801 g          542 t
ORIGIN      
        1 tcttcgacgc gaaatccgtg tctatagtaa gacggcggcg caggtgtttc cgggcgaggg
       61 cggcatcaat ccacgaaaga actcgaacta tggccctgac gtgaatctcc ccgtgccgca
      121 ggccgagcga aacaacttca agttcggcgg atgtacggac cggaaagcat aagacaacag
      181 ggttggccgt agagaatgaa agcgggagca cgcgtcgcgt gctcccgctt tctgtttgat
      241 ggcgcggtgt tacggcttcg tcgcctgaag agtgatttct ttcaatgcat caccgtccgc
      301 tggcagcgcg gcttggccag cgggaaagcc gttgaccttc agcagaaagg ccatcacgtc
      361 ggtgatgagc tgacggtcgt agataccgac gctgtcggtt ggcatcgtcg ttttgatcct
      421 ggtctgcaga tcgctcaacg gcaagccatt ccagtttcct aaaaacgcgc cgcccgtcag
      481 tggtggtgat tcgtcgcccc cagtgagcga tgctccatgg cagcgcgcgc aggtcttggc
      541 gtacgtgctt tctccgcgcg ccgcttgtgg caccgagtac acgctgtccc acactgtgcg
      601 cgaagccgac gggcgcgcgg acgttggcga tttggaactc atggccaacg cgggtaccat
      661 cgcgatgagc gcgcaggcgc tcaaccatcg gctcgaaaca aactgtgact gactgcgctt
      721 catcgctgga tcacttcggt gcagcgtccg gaaggcgcag cgcaatgtat tcgccggagt
      781 aggggccgcc gctcaccgcg gttacgatgt actgcttgcc gtttaccgag tacgtcatgg
      841 gcgatccact gatcggcgcc ggcagcaaga ccgatccgac ttcggcgccc gttgccttgt
      901 cgtacgcgcg aagcatcgcg ccacgcggcc gcgtcggcag tgtcgtgact tgcggatcgc
      961 cggcgatcac caaggtcttc gtgacgagtg tgccaacgat gaatccaggc tgaccggtcc
     1021 ggggaatcgt catgcctttc agcgcgtcac tgttgcggac agcgtcgggt gtctcgccgt
     1081 gcggcacctg ccaggcaatt tcgcctttgt cgagattgat cgcgctgatc gtaccgtatg
     1141 gcggcttgaa gatcggaagg ccttggatcg agagggccgc gccgccgcgt ccgcctcccc
     1201 ctccggtcgc accaggcgcc gatgcgatgc cggcgatcca actcatgtcc gaacgctcct
     1261 tgctgggttg gaccagccca aggacgaccg gcgccgcgtt ggacgatgcc agatacacca
     1321 tatgagtttc tggatcgtac gatccgccgg gccaattgga gccgccgttc gtcgggccgc
     1381 gagcgagtgt gccgatcggg ccttccgctt tgctgacgac gggcggtgtg aagattggtc
     1441 cgagtttgaa gcgcgacgcg atcgcgagag cctgtgcatg aagctcggga gtgaagtcga
     1501 ccaaatcgtc cgtcgagatt ccgtttttcg cgtaggcgcg cggcttggtt ggaatcggtt
     1561 gcgttggtga gtaccactcg ccgggcacgt cgcctttctc gacgggcttc tcctcgaatg
     1621 gccacaccgg ttttcccgtc acgcggtcga agacatagag aattccttgc ttcgtcggga
     1681 cggccacggt cttgaccggc ctcccgtcga catttgcatc ggcgagaatc ggcgcggacg
     1741 cgaggtcgaa gtcccaaatc ggatggtgca cgatctggta atgccacttc tttgcacccg
     1801 tccgaagatc caagcagacg aggctctcgc cgtacagatc gttgcccggt cgatggccgc
     1861 cgtagtaatc accggtgggc gattccaccg gcagatacac gaggcccagg tcttcgtcga
     1921 tcgtgacttg cgtccacacg ccggtgttgc cggcgtcctc ggcggatcca ttcagccacg
     1981 tctcgtatcc cgactcccct ttctttggaa ttgtgtgaaa ggtccagaga cgtttgccgg
     2041 tgcgcacgtc gaaggcgcgg atgtagcctt tattgttctt ataactcttt ggtgcgctgc
     2101 cttccttgaa cgccgcacca atgacgacga catcgcggcc aacgacgggc gcaccctgat
     2161 agccgatctc tcctgtgatg aggtctggga aaatcgtctg atcgacgtcc gtcttgagat
     2221 cgaccacgcc gttcgtccca aagctcggaa tcacttcgcc ggtcttggcg tccaatgcca
     2281 tcagccgata gccaggcgtg acgtagatga ttcgttcgtc gcgaccatcg gtccaatacg
     2341 cgagtccgcg accggacagt ggccgcggtg ccgccgcgcc gcgctcacct tcatcttctc
     2401 gatgcaccca tttctgcgcg ccggttacgg catcgagtgc aacgacggca cggcctgttc
     2461 cgccagtcgt gtagagcgtg ccgcggacca tcaacggcgt tccctcgagc ttgaactccg
     2521 ggcgaggtcc aagcgagtca gtcttgaacc gccatgcgac ctcgagcgtg ctgaaattcg
     2581 ctgcgttgat ctggtcgagc
//
LOCUS       DATIMH010000194         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4664450, whole genome shotgun sequence.
ACCESSION   DATIMH010000194 DATIMH010000000
VERSION     DATIMH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4664450"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VLI90_03310"
     CDS             complement(<1..1187)
                     /locus_tag="VLI90_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSV13257.1"
                     /translation="MPVGTVTLMFTDIEGSTSLWEKLGDRFYPVLEQHNVILRDAIAA
                     CRGFEVKTEGDAFMVAFQTAQDGVRCAIAAQLALAEAHWPADVGEVRVRMGLHTGEPI
                     VATHPDGARDYFGPMVNRSARVAASAHGAQVVLSDAARLAAGQLPDGATLADLGRHQL
                     KGLDQLDQIWQLDHPSFRIKNFPPLKTLNAVRHNLPLPVTSFIGRKTEVDELQKLIAR
                     PEIRLITMLGPGGTGKTRLSQHVAAEMAEGFKDGVWFVELAETKTAVEIPQAVLRAMS
                     IRPLPTRDMRDQLFEHLASKHLLLVLDNTEQIADIAAFVTDVQRAAPEIELLVTTRIM
                     LNLRGEHVYTVPPLPLPDEGETRIDRLTQSDAVCLFAERARDACTAFELTPENGPLIG
                     TLV"
     gene            1685..>1917
                     /locus_tag="VLI90_03315"
     CDS             1685..>1917
                     /locus_tag="VLI90_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13258.1"
                     /translation="MAAGAAVALANHAHVFPPAMNADGTVDQLLRHLDACDIARAVCF
                     APFPFQSKDPSFDQNEWLAREIKARDRLYAFGTL"
BASE COUNT          335 a          574 c          663 g          345 t
ORIGIN      
        1 tgcaccagcg tgccgatgag cgggccgttt tcgggggtca gttcgaaggc ggtgcacgcg
       61 tcgcgggcgc gctcggcgaa gaggcagacg gcgtcgcttt gcgtcaggcg gtcgatgcgc
      121 gtttcgcctt cgtccggcaa cggcagcggg gggacggtgt agacgtgttc gccgcgcagg
      181 ttcagcatga tgcgcgtcgt caccagcagc tcgatctccg gggcggcccg ctgcacgtcg
      241 gtgacgaacg cagcgatgtc ggcgatctgc tcggtgttgt cgagcaccag cagcaggtgc
      301 ttgctcgcca ggtgctcgaa gagctgatcg cgcatgtcgc gcgtcggcag cgggcggatg
      361 ctcatcgcgc gcagcacggc ctgcgggatc tccacagcgg tcttggtctc ggccaactcg
      421 acgaaccaga cgccgtcttt aaacccttcg gccatctcgg cggccacgtg ctgcgacagg
      481 cgcgttttgc cggtgccacc ggggccgagc atcgtgatca gccgaatctc cggccgcgcg
      541 atcagctttt gcagctcatc gacttcggtc ttgcggccga tgaagctcgt caccggcagc
      601 ggcaagttgt ggcgaaccgc gttgagcgtt ttaagcggcg ggaaattctt gattcggaag
      661 ctgggatgat cgagctgcca gatctggtcg agctgatcca gtcctttaag ttgatgacgg
      721 cccaggtccg ccagcgtcgc cccatcaggc agttgtccgg cggcgagacg cgcggcatcg
      781 gacaaaacga cttgagcgcc gtgagcgctc gccgcgacgc gcgcggagcg attcaccatc
      841 ggcccgaaat aatcgcgcgc gccatcggga tgcgtcgcga cgatcggctc cccggtgtga
      901 aggcccatgc gcacgcggac ctcgccgacg tctgccggcc agtgcgcttc cgccagcgca
      961 agttgggcgg cgatggcgca gcgcaccccg tcttgcgcgg tttgaaaggc gaccatgaag
     1021 gcgtcacctt cggtcttcac ttcgaagccg cggcaggcgg cgatcgcgtc gcgcaggatc
     1081 acgttgtgct gctcgagcac ggggtaaaaa cgatcgccga gtttctccca aagcgaagtg
     1141 gacccttcga tgtcggtgaa catcagggtc acggttccaa ccggcataag tgcgggatca
     1201 cccaagccct gtgagcgtaa gcaatcgcgg gggggcagac aagaaaattt aaaagggggt
     1261 aaatggtggg gcttgggagg ctgggccggt ctttgcgcga gtgtgtcccc cggcagagcc
     1321 gggggctttg ggcggtgaca aaatttttga cggcgcgcgc aggatggcgg gtcttgctgg
     1381 gtatctcatg catcgaaacg atcggaaagg gacggcaaca ccgaacgagc gccgtccacc
     1441 ccgttccgtc gcggtctgcg ggagtgtctg gctgttacgt gggtgaccag gcatcaatcc
     1501 ttcgcaggct gcgacgggcg gggcggatag gcgaacgctg attgttgatc gatgcgattt
     1561 cgcgttgaac gtggcacatc tgatatgatg gcggcctgcc acacgcgggg gccgacaact
     1621 agaagcgggc ggatgttcgc gcggggaaat gcgcgcgtgc caccgtcgcg gaggcgaaca
     1681 caagatggca gcaggggccg ccgtggcgct ggcgaatcac gcgcacgtct tcccgccggc
     1741 gatgaacgcc gacgggaccg tcgatcaatt gctgcgccat ctcgacgcct gcgacatcgc
     1801 gcgcgcggtc tgctttgcgc ccttcccgtt tcaatccaaa gatccaagct tcgatcagaa
     1861 cgaatggctt gcgcgcgaga ttaaagcgcg cgatcggctc tacgcattcg gcacgct
//
LOCUS       DATIMH010000195         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1775631, whole genome shotgun sequence.
ACCESSION   DATIMH010000195 DATIMH010000000
VERSION     DATIMH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775631"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2089
                     /locus_tag="VLI90_03320"
     CDS             <1..2089
                     /locus_tag="VLI90_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13259.1"
                     /translation="TQPAMRPTMVATTSPAAKLVATYMDAVRTANPLVPATQPLALPV
                     DLSDAAHLIVHDAVYLDPLGHLWIARRDARPTAEALRNPADVTEHVVADAPAFVHWWI
                     DGNGDWAAAVIARGSSNSGGWDIITLRDRAPLGADRPFHWQRAYSWDGKFVVPSDGGV
                     SVFEMDGPSRFVEHYHALAGAPASPPLTVLDAKGVLAWSPWERGQTGSRGASRYVDGS
                     WSDLSPENWGERFVQLIPLLDGSVLQIIAGAGAGEDDHVSMSIAPLESTQIDQHHVEE
                     LVTALADPDDAKRHSAFDELSRYGPGLWPILEKLADDQPAEARVAIKQLLRSKTAPGL
                     GGLTLIGDRLTVVNRQNDGSVLLFAPLGVKIGNGDSEPQVVTPAWISLRPGGRVERPL
                     PVALVKDLRADLPCHLRAVRDDWIISDEGGGPRRFVGNGFVPLVRPDERTFTQLIGED
                     RRGRWVFSKPSSSEASTRASASTQTSVAETLIIDPTILDPAPRLPVWTMTIQKGVVGW
                     DGGDWPVIKRGGAWALKSDSWEPLPEQSAIITEMPALPSAHHAATQPTTAESLGRPLR
                     VTSDGTRYYDGRTWLVTIDKSGHQTRWPLPASAVGSMDPPTLIRTADGQLFLFNQPGR
                     VLRIAPTPEGSEPFRVEATFTKDIPNVDHPARIWLDPLGRIDIAYNANCLAVLFPSGH
                     IPMEISQMMDSKN"
     gene            2165..3004
                     /locus_tag="VLI90_03325"
     CDS             2165..3004
                     /locus_tag="VLI90_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV13260.1"
                     /translation="MSEERFRGGSRRAFTLLEVLIVIGIISVLMAILLPAAERVRHQA
                     YIDKCASNLRQICLAFQTYAQDNHGNYPRTPYSGNPATYVLNEGTGTNAVDPFQSGPG
                     AVQVNDLTAPLFLLMKMQKLSPEVMICPYNDETDFKADNSNFTGRSNFTDEKKNLGYS
                     FANPYPLDAVAAAGYRLSNKLSAEFAVAADRNPGVSGKNDDVYAPTLGSPWSKMKLAN
                     SPNHEFDGQNVLYGDGHVAWQKTAFCGVAQDNIYTAKKGLAPKVEASPSDATDSILLP
                     TDD"
     gene            complement(3112..3369)
                     /locus_tag="VLI90_03330"
     CDS             complement(3112..3369)
                     /locus_tag="VLI90_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13261.1"
                     /translation="MRADEMESPQSGATETLAYATPDHRRGQSAGGTVGVILVALFIC
                     IAAVAIFALVIHEHQEANAAALAVSALAAMGTCCAFFLTRR"
     gene            complement(3356..3622)
                     /locus_tag="VLI90_03335"
     CDS             complement(3356..3622)
                     /locus_tag="VLI90_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13262.1"
                     /translation="MSRSWFKAKRFGWGWTPTTWQGWFVMAAFVAAVTTSAFGILGVG
                     KVMTIHQWVAFYAALALEPLILVLICCKTGERPRWRWGTHDESR"
     gene            complement(3805..4092)
                     /locus_tag="VLI90_03340"
     CDS             complement(3805..4092)
                     /locus_tag="VLI90_03340"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HSV13263.1"
                     /translation="MIMGFRVHDVQPTPNPNAVKFILDRPVSDQPMSFFNAGAAKDHP
                     LAARLFGVPGVSSLLLLGDFITINKSPEARWADIKGKVEQVLAGTSPEGLK"
BASE COUNT          812 a         1296 c         1420 g          808 t
ORIGIN      
        1 cactcaaccg gcgatgcgac caaccatggt cgccaccact tcaccggcgg cgaagctggt
       61 cgccacttat atggacgcgg tgcgcaccgc gaaccccctg gtgccggcga cgcagccgtt
      121 ggcgttgccg gtggatcttt ccgacgcggc ccacctgatc gtgcacgatg cggtttatct
      181 tgacccgctg ggtcatcttt ggattgctcg gcgcgatgca cgacccaccg ccgaggcgct
      241 gcggaatccg gcggacgtga ccgagcacgt cgtcgccgat gcgccggcgt tcgtgcattg
      301 gtggatcgac ggcaacggcg attgggcggc ggcggtgatt gcgcgcggaa gctcgaactc
      361 gggcgggtgg gacatcatca cgctgcgcga ccgcgcgccg ctgggcgccg atcggccctt
      421 ccattggcag cgggcatatt cgtgggacgg gaaattcgtc gtgccgagcg acggcggggt
      481 ttcggttttt gagatggatg ggccgtcgcg tttcgtcgag cattatcatg cgctggcggg
      541 cgcaccggcg tcgccgccgt tgacggtgtt ggatgccaag ggtgtgctgg cgtggtcgcc
      601 atgggaacgc gggcagaccg gcagccgcgg ggcgtcgcgg tatgtggatg gcagttggtc
      661 cgacttgtcg cccgagaact ggggtgagcg attcgttcag ctgatcccgc tgctggacgg
      721 gagcgtgctg cagatcatcg ctggcgcggg cgcgggtgaa gatgaccatg tgagcatgtc
      781 gatcgcgccg ctggagtcca cgcagatcga tcagcatcac gttgaagaat tggtgacggc
      841 cttggccgat ccggatgacg ccaagcggca cagcgcgttt gatgagttgt cgcgctatgg
      901 gccggggctt tggccgattt tggaaaagct ggcggacgat caaccggccg aggcgcgcgt
      961 ggcgatcaag cagttgctgc gcagtaagac ggcgccggga ttgggcgggc ttacgctgat
     1021 cggcgatcga ttgacggtgg tcaatcggca gaacgatggg tcggtgctgc tgttcgcgcc
     1081 gctgggggtg aagatcggga atggagacag cgagccgcag gtcgtcacgc ccgcgtggat
     1141 cagccttcga cccggcggcc gcgtcgagcg gccgctgccg gtggcgctgg tgaaggacct
     1201 gcgggccgat ctcccctgcc acctgcgcgc ggtgcgcgat gattggatca ttagcgatga
     1261 aggcggcgga ccgcggcggt tcgtcggcaa cggtttcgtg ccgctggttc gaccggatga
     1321 gcgcacattc acgcagctga tcggcgaaga tcgccgcggg cgatgggtgt tttctaaacc
     1381 gtcatcgagc gaggcatcga ctcgcgcctc agcatcgaca cagacttcgg tcgccgagac
     1441 gttgatcatc gatccgacga tcctcgaccc cgcgccgcgg ttgccggttt ggacgatgac
     1501 catccagaaa ggcgtggtcg gctgggacgg tggggattgg ccggtcatca aacgcggcgg
     1561 cgcgtgggca cttaaatcgg actcgtggga accgctgccg gagcagtcgg cgatcatcac
     1621 ggaaatgcct gcgctgccaa gcgcgcatca cgcggcgacg cagccgacga cggcggagtc
     1681 gctcgggcgg ccgctgcgcg tgacgtccga tgggacgcgc tattacgacg gtcgcacgtg
     1741 gctggtcacg atcgacaagt cgggccacca gacgcgctgg ccgctgccgg cgtcggcggt
     1801 gggcagcatg gatccgccga cgctgattcg cacggcggac ggacagctgt ttctgttcaa
     1861 ccaaccgggc cgggtgctgc ggatcgcgcc aacgccggag ggcagcgagc cgtttcgggt
     1921 cgaggcgacc ttcaccaagg acattcccaa cgtcgatcat cccgcgcgca tctggctcga
     1981 tccgctgggg cggatcgaca tcgcctacaa cgccaattgc ctggccgtgc tgtttccgtc
     2041 cggccacatc ccgatggaga tttcgcagat gatggacagc aagaactgag catgcgtcat
     2101 tttcctgtca tgtcgcacga gtagcttttc cttcgatgcg cacgcgaagc cgcgaaggaa
     2161 gtctatgagc gaagaacgat tcaggggcgg tagccgccgc gcgttcacgc tgctggaagt
     2221 gctgatcgtc atcggcatca tctcggtgct gatggcgatc ctgctgcccg cggcggagcg
     2281 cgtgcggcac caggcgtaca tcgacaaatg cgccagcaat ctccgccaga tctgtctcgc
     2341 gtttcaaact tacgcgcagg acaaccacgg caactacccg cgcacgccgt atagcggaaa
     2401 ccccgcgacg tatgttttga acgaaggcac ggggacgaac gctgtcgatc catttcaatc
     2461 cggcccgggc gcggtgcagg tgaacgacct gacggcgccg ctgtttctgc tgatgaagat
     2521 gcagaagctg tcgccggagg tgatgatctg tccgtacaac gacgagacgg atttcaaggc
     2581 ggacaattca aacttcaccg gccggtcgaa ttttacggat gagaagaaaa atctcggcta
     2641 cagctttgcc aatccgtatc ccctggacgc cgtggcggcc gccggatatc ggctgtcgaa
     2701 caagctgtcg gcggagtttg ccgtcgcggc cgatcgcaac cctggggtga gtgggaagaa
     2761 cgacgatgtg tacgcgccga cgctggggag cccgtggtcg aaaatgaagc tggcgaacag
     2821 cccgaaccac gagttcgacg ggcagaacgt gctgtacggc gacgggcacg tcgcgtggca
     2881 gaagaccgca ttctgcggcg tcgcgcagga caacatctac acggcgaaga agggcctggc
     2941 gccgaaggtg gaggcgagtc cgtcggatgc gacggacagc atcctgctgc cgacggatga
     3001 ttgagaagcc actcgcgcgg tgagaaccat caccgtgaca cgggcctccg gctcgagtca
     3061 cggtgacccg ttcaaatttc aaatcatttc ttaccgatcg acatttgcac gtcatcgccg
     3121 ggtgaggaag aacgcgcagc aggtgcccat cgccgccagc gcgctcacgg cgagcgcggc
     3181 ggcgtttgct tcctggtgtt cgtgaatgac cagagcaaag atcgcgacgg cggcgataca
     3241 gatgaacagc gcgaccaaaa tcacgccgac cgtgccgccg gcgctctgcc cgcggcgatg
     3301 atccggcgtt gcatacgcca gcgtctccgt cgctccggac tgcggcgatt ccatttcatc
     3361 ggctctcatc atgtgttccc catcgccacc gcgggcgctc gccggtcttg caacagatca
     3421 acaccaggat cagcggctcc agcgccaacg ccgcgtagaa cgcgacccat tggtggatcg
     3481 tcataacctt ccccacgccg aggatgccaa aggctgatgt ggtgacggcg gcgacgaacg
     3541 cggccatgac aaaccaaccc tgccatgtcg tgggtgtcca tccccagccg aaccgcttgg
     3601 ctttgaacca cgaccgactc attagcgaaa ttctatcagc aggtcgaact ggctggccag
     3661 tatgacggta tacgtcaaaa atacgcgcat aaaaaaaccc cgtaggcgtg ggtgagacct
     3721 acggggtaag gagggaagaa agctgttacg cacgagtcat acgtgcggcg tttgattgta
     3781 ggtcgcgcga accggacaaa cttcctattt taaaccttcc ggtgacgttc ctgcgagcac
     3841 ctgctcgact ttgcctttga tatcggccca gcgagcttcc ggacttttgt taatcgtaat
     3901 gaaatctccc aggagcagca ggctgctgac cccgggcaca ccaaacaacc ttgcagccag
     3961 cgggtggtcc tttgccgcgc ccgcgttgaa gaagctcatc ggctggtcgg aaaccggccg
     4021 atcgaggatg aacttcacag cgttcgggtt cggcgtgggt tgaacgtcat gaacccgaaa
     4081 gcccatgatc attcctcatc atcctccgct ggctaactat accatgctcc gtatcgcctt
     4141 cgccaccgct agattcgcta ttttgacgct ttttgcaaaa ttttttattc cctcgcaaca
     4201 ggggcgggcc cgcggcgttt agagttaact gcgcacttgt cggttcgtgg gtcacttgga
     4261 acgtcatcat tcctgtagct gcgacgcctg cgtcgcctct ttcggcccga ggcgacgcag
     4321 gcgtcgcggc tacaat
//
LOCUS       DATIMH010000196         3415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4665305, whole genome shotgun sequence.
ACCESSION   DATIMH010000196 DATIMH010000000
VERSION     DATIMH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4665305"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VLI90_03345"
     CDS             <1..1765
                     /locus_tag="VLI90_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13264.1"
                     /translation="ACEFPDGNYYGQQCCIKAIETLSAMDEVGIVSFSWNGGGSNWDF
                     PLAPKGDGSKAVAAAKAMNVGDMPSFEESMAVALHGSATSKGLKDSDAKQKHVIVISD
                     GDPGPPSPGLVAEYNAAKVTVSTVSVYPHNGDPDGLPITMKGIARDLHGKAYGPINNN
                     PNQLPQIFVKEATVVRRSLITEDNNGIQLKRTPSSSDMIKGLSEIPAVKGMVLTSRKN
                     NPQIEIPLVAGKNNDPVLAHWQTGLGRAAVYTSDANNKWGAWWVQSPDFNKFWAQVVR
                     GVARPPMSTLFQITTSQEGDKAKIVVEALDKDSGFQNFLNIAGRVAGPDPGKPPIDVR
                     LVQTGPGRYETTFDTPEPGTYVSALQYHGANGQTGMLLSGVAMNNSPELRDLQSNDGL
                     LREIAERTGGRVLTPFDVQNANIFDHEGLPPAISPLPVWDVLLPFLIALILIDVATRR
                     IAWDWVALKRYAAVTTSAVRSYTTTRKIETRESLDALKRIREEGAAAAPQPGLSASAV
                     SSPAPARPDPKAKFEAPKTTQQVEGDITKVVGGATDKPMPAPPKKVEPKGAQGGGMGS
                     LMEAKRRAQQKIKEKEQGEER"
     gene            1769..2044
                     /locus_tag="VLI90_03350"
     CDS             1769..2044
                     /locus_tag="VLI90_03350"
                     /inference="COORDINATES: protein motif:HMM:NF023528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5-related ribonuclease"
                     /protein_id="HSV13265.1"
                     /translation="MASADAPDGTTGGADPPLRFSNEIKLRSQMRRRGWSDQMIREAM
                     QAPGIPTNGKKGPATRYVHPTTGRSVVVDNATGEIFHVGGEEFRYDD"
     gene            2034..2282
                     /locus_tag="VLI90_03355"
     CDS             2034..2282
                     /locus_tag="VLI90_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13266.1"
                     /translation="MTTDREQVFVYLEDEGVEVWRPTQAERQSDGSYRLLPTPDYDPN
                     DEKWQFPPGSRVWCERRKLSGGEALVAARAADSKKRTA"
     gene            2309..3376
                     /locus_tag="VLI90_03360"
     CDS             2309..3376
                     /locus_tag="VLI90_03360"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSV13267.1"
                     /translation="MTQQNPIPGVDPQIQSLTDQFRKDYHAIQAEIAKAIVGHADIVD
                     GVLTCLFVGGHALLEGVPGLGKTALIRSLAQALNLKFNRIQFTPDLMPADVIGTNIIM
                     ENAQGLKAFQFMHGPIFSQIVLADEINRATPKTQSALLEAMQEKQVTAGGEIRHLELP
                     FFVMATQNPLEQEGTYPLPEAQLDRFFYKLLVQYSGRDELRQILDRTTTNYKDEPQPV
                     LDGPRIMQHQQLIKRVVIAPHVQDYAIRCTLATHPNGIFAIPMVNQFLRVGASPRAAQ
                     AITLASKVRALLDGRFHVSFKDIKEVVVPAMRHRVILNFEGEAEGITTDMVLEKVIAE
                     TPTTIEGESAAAPVPAGAARR"
BASE COUNT          771 a         1121 c         1035 g          488 t
ORIGIN      
        1 cgcctgcgaa ttccccgatg ggaactatta cggccagcag tgctgcatca aggcgatcga
       61 aacgctgagc gcgatggacg aagtcggcat tgtcagcttc agctggaacg gcggcgggag
      121 caattgggac tttccgctgg cgcccaaagg cgacggcagc aaggccgtcg ccgcggccaa
      181 ggcgatgaac gtcggcgaca tgcccagttt cgaagaatcg atggccgtcg cgctgcacgg
      241 gtcggccacc agcaaggggc tcaaggacag cgacgcgaag cagaagcacg tgatcgtcat
      301 cagcgacggt gaccccggcc cacccagccc cggcctggtc gctgaataca acgcggcgaa
      361 ggtcaccgtc agcacggtca gcgtctatcc acacaacggt gatcccgacg gcctgcccat
      421 caccatgaag ggcatcgcgc gcgacctgca tggcaaggcc tatggcccga tcaacaacaa
      481 cccgaaccag ctgccgcaga tcttcgtgaa ggaagcgacg gtcgttcggc gaagcttgat
      541 caccgaagac aacaacggca ttcagctcaa gcgcacgccc agcagcagcg acatgatcaa
      601 gggcctgagc gaaatccccg cggtgaaggg gatggtgctc accagccgaa agaacaatcc
      661 gcagatcgag attccgctcg tcgccggcaa gaacaacgac ccggtgctgg cccactggca
      721 gacgggcctc ggtcgcgccg ccgtttacac gtccgatgcc aacaacaagt ggggcgcttg
      781 gtgggtgcag tcgcccgact tcaacaagtt ctgggcccag gtcgtccgcg gcgtggcgcg
      841 cccgccgatg tccacgctgt tccagatcac caccagccag gaaggcgaca aggcgaagat
      901 cgtcgtcgaa gcgctggaca aggacagcgg gttccagaat ttcctgaaca tcgccggccg
      961 agtcgccggg cccgatccgg gcaaaccgcc gatcgatgtt cgacttgtcc aaaccgggcc
     1021 gggccgatac gagacgacct tcgatacgcc cgaacccggc acgtacgtct ccgcattgca
     1081 ataccacggc gccaacggcc agaccggcat gttgctgagc ggcgtcgcaa tgaacaacag
     1141 cccggagctg cgcgacctgc aaagcaacga cgggctattg agggaaatcg ccgagcgcac
     1201 cggcggccgg gtgctcacgc cgttcgacgt gcagaacgcc aacatcttcg accacgaagg
     1261 cctgcccccg gcgatcagcc cgctgcccgt ctgggacgtg ctcctgccgt tcctgatcgc
     1321 gctgatcctg atcgacgtcg cgaccaggag aatcgcctgg gactgggtcg cgctgaagcg
     1381 ctacgccgcc gtcaccacca gcgccgtccg ctcgtacacg accacgcgca agatcgaaac
     1441 cagggaatcg ctcgatgcgc tcaagcgcat ccgagaagaa ggcgccgccg ccgctcccca
     1501 acccgggctg tccgcttcag cggttagctc tccagccccc gcgcgtcccg accccaaggc
     1561 gaaattcgaa gcgccaaaga caacacaaca agtcgaaggc gacatcacca aagtcgtagg
     1621 cggcgccact gataaaccca tgcccgcgcc gcccaaaaaa gtcgaaccca aaggcgccca
     1681 aggcggcggc atgggcagcc tgatggaagc caaacgccgc gcgcagcaga agatcaagga
     1741 gaaggaacag ggagaggagc gatagaatat ggcgtcggct gatgcgcccg atggcaccac
     1801 tggtggcgcc gatccaccgc ttcgattcag taacgaaata aagttgcgca gccagatgcg
     1861 aaggcgcggc tggagcgacc agatgattcg cgaggcgatg caggcgcccg gcatcccgac
     1921 gaacggcaag aaagggcctg cgacgagata cgtccatcca acaaccggcc gttccgtcgt
     1981 ggtggacaac gcaactggcg aaatctttca cgtgggtggc gaggagttca gatatgacga
     2041 ctgatcgcga acaagtgttt gtctatctcg aagacgaagg cgtggaagtg tggcgaccga
     2101 cgcaggccga gcgccagtcc gatggaagct accggctgct gccgacgccc gattacgatc
     2161 ccaacgacga gaagtggcaa tttccacccg gcagccgcgt gtggtgcgag cgccgaaagc
     2221 tttccggcgg cgaagcgctc gtcgcggcgc gcgctgcgga ctcaaaaaag cgaaccgctt
     2281 aactctcacc ggctctgcac gaggaaacat gacccagcaa aaccccatcc ccggcgtcga
     2341 tccgcagatc caatccctca cggatcaatt tcgcaaggac tatcacgcca tccaggccga
     2401 gatcgccaag gcgatcgtcg gccatgccga catcgttgat ggcgtgctca cctgcctctt
     2461 cgtcggcggg cacgcgctgc tcgaaggcgt ccccggactc ggtaaaacgg cactcatccg
     2521 ctcgctcgct caggcgctga acctgaagtt caaccgcatc cagttcacgc ccgacctgat
     2581 gccggccgac gtgatcggca ccaacatcat catggaaaac gcccagggcc tgaaggcgtt
     2641 ccagttcatg cacgggccga tcttttcgca gatcgtgttg gccgacgaga tcaatcgcgc
     2701 cacgcccaag acgcagagcg cgctgctgga agcgatgcag gaaaagcagg taaccgccgg
     2761 cggcgagatc cgccaccttg aattgccgtt cttcgtgatg gcgacgcaaa acccgctgga
     2821 acaggaaggc acctacccgc tgcccgaagc gcagctcgac cgcttcttct acaagctgct
     2881 cgtgcaatac agcggccgcg atgaactgcg gcagatcctc gaccgcacga cgacgaacta
     2941 taaagacgag ccgcaacccg tgctcgatgg cccgcgcatc atgcagcacc aacagctgat
     3001 caagcgcgtc gtgatcgccc cgcacgtgca ggactacgcg atccgctgca cgctcgccac
     3061 gcaccccaac ggcatcttcg cgatcccgat ggtgaaccag ttccttcgtg taggggcaag
     3121 cccgcgagcc gcgcaagcga tcacgctggc gagcaaagtt cgcgcgctgc tcgatggccg
     3181 gttccacgtc agcttcaaag acatcaagga agtcgtggtg ccggcgatgc gccaccgcgt
     3241 gatcctgaat ttcgaaggcg aagccgaggg gatcacgacg gacatggtgc tggaaaaggt
     3301 gatcgcggaa acgccgacga cgattgaagg tgaaagtgca gcagcgccgg tcccggcggg
     3361 cgccgccaga agataagcat ccacccggcg gtgccgcttc agcggcactg ggcgt
//
LOCUS       DATIMH010000197         4674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5530983, whole genome shotgun sequence.
ACCESSION   DATIMH010000197 DATIMH010000000
VERSION     DATIMH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5530983"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VLI90_03365"
     CDS             complement(<1..913)
                     /locus_tag="VLI90_03365"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HSV13268.1"
                     /translation="MNGFQVVSPYKPEGDQPAAIDKLVAGVNEGKRFQTLMGVTGSGK
                     TFSMAHTIARLNRPTLVISHNKTLAAQLYEEFKDLFPHNAVGYFVSYYDYYQPEAYIP
                     QRDIYIEKDASRNDDLDRLRLSATSNLVSRRDVLLVASVSCIFGLGSPAEYKSSVLPI
                     RKDVETDRDEMLRKLIDLQYSRNDIDFKRGTFRVRGDVVELHPSYEEFAYRIEFFGEE
                     ITNIDAINPLTGELLKSEDQIFIYPAVHYVMPEERVHEAVKNIREELDQQVMHLRGQG
                     KLLEAQRLLARTKYDLEMMLEVGYCSGI"
     gene            complement(1070..3094)
                     /locus_tag="VLI90_03370"
     CDS             complement(1070..3094)
                     /locus_tag="VLI90_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13269.1"
                     /translation="MQKVTAILEKNVEWVALGIAGLFFLFMLYSYIVLPPVTATVGSA
                     PVTPGEVDPKILAGPAKDLERKMSTAEVPKFVVKDFGQDLQEVLANKNVEIQAIAGAY
                     GPSNIAPEQLQARPQPGTPGVAVVPGQPGQPGQPNPTVAAAAPVTVLPVPPAPANLQF
                     SQGRSNVTIPAPAAPGSPNPAASTGTPTDRNWVTVSGLIPMKGLADEWTKRNIPPQPN
                     TTTILRVEMVRQELDPTTDNWGPETVLPPLQNVNLQPLPPVNANPTQAQTYQQWAETT
                     QIDLLQPPFYAVTQGDPWLFPGQALLQPDQINQAFDPSKVQAKDIATLTPDQKKLYDD
                     YRKAEAERKRQEQRQRYQQTHPKSGGGGGGAAPYGPPGGRGGPGGDSTSYAPRDPERP
                     ITLAQVGRGRISFDPEMEAAPVPYGPGGGPAAPNQPPPQPGQPVPGPVPPIGPVPNPG
                     SFDPIALSNGQLPNGQQPPPAPVPVPAQPGAAPAAPALNPGDVTIWAHDDSVQPGHTY
                     RYKLRYIIRNPVFQTRNVCKPQTLADQFLINSTESDWTPRVSVKSETNLFAASTSPLG
                     DRVKFDIFRWKNGNWQMQTVEVTPGDMVGSTQGQGPAAVDFKTGLTLVDVRQDPKNSG
                     NRIILLTGDNGQIVLHNTVDDRNSDERKRLMDEIQKNVKPPGNPTASAGT"
     gene            complement(3161..4507)
                     /locus_tag="VLI90_03375"
     CDS             complement(3161..4507)
                     /locus_tag="VLI90_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13270.1"
                     /translation="MNTAQILEILKKNIFSVVCAVIAIAAVVATFWPIGGMYAQLKTQ
                     ADQHASMYSQLQGLITKERKLPQTDPAQTTQDPLTIFPNEQTITKGAEATKRVSEESK
                     HMVEFVDKINRDPHNLLVQDALPNPATDTPRFIFGDLYKLVLSMDPAVSIKTEPKLQN
                     AKAPNIRNDILNAGVPPTDVEITTRKNDIWNNDFAPRVITVNGQPVNLPQVQQDFTNR
                     SLTLPDEMKMEVAKTKRIYIAPDALTMNPNVIANNAPAPFDIWYAQLSLWIQQDVATA
                     IAQANAKSKNILDAPVKHFLKLTIPPAPGTYVLQPNTATPGAPPAAAPVEGGDTQPVP
                     KVPAVSATGRVSNPMYDVVQFKLAINVDAAQVPYVIAMLGQNRLIDVYNIDVVTVDSS
                     ERANAGFIYGANPVVQLNLKCETLFMRQWTVPWMPDRVRVAIGVPPVAPVVGGATP"
BASE COUNT          716 a         1433 c         1545 g          980 t
ORIGIN      
        1 cgatccccga gcagtagccc acctccagca tcatctccag gtcgtatttc gtgcgcgcga
       61 gcagccgctg cgcttcgagc agcttgccct gtccgcgcag gtgcatcacc tgctggtcca
      121 gctcttcgcg gatgttcttc accgcttcgt gcacgcgctc ttcgggcatc acgtagtgaa
      181 cggcggggta gatgaagatc tggtcttcgc tcttgagcaa ctcgccggtc aaaggattga
      241 tcgcgtcgat gttggtgatc tcttcgccga agaattcgat gcgataggcg aattcctcgt
      301 aggacggatg cagctcgacg acgtcgccgc gcacgcggaa cgtgccgcgt ttgaagtcga
      361 tgtcgtttcg gctgtactgc aggtcgatca gtttgcgcag catctcgtcg cgatcggtct
      421 cgacgtcttt tcgaatcggc agcacgctgc ttttgtattc cgcgggcgaa cccagaccga
      481 agatgcacga gacggatgcg acgagaagca cgtcgcgccg gctgaccagg ttgctggtcg
      541 ccgagaggcg caggcggtcc aggtcgtcgt ttcggctggc gtccttctcg atgtagatgt
      601 cgcgctgcgg gatgtacgct tcgggctggt aatagtcgta atagctgacg aaatagccga
      661 ctgcgttgtg cgggaacagg tctttaaatt cctcgtacag ctgggccgcc aaagtcttgt
      721 tgtggctgat gaccagcgtc gggcggttca gccgcgcgat ggtatgcgcc atcgagaacg
      781 tcttaccgga accggtcacg cccatcaggg tttggaagcg tttgccttcg ttgacgccgg
      841 cgaccagctt gtcgatggcc gcgggttgat cgccttcggg tttgtaggga ctgacgactt
      901 ggaatccatt catgcggggg attatacgct ggtgaagggg atggttcagg cgcggcggcg
      961 cgatggagcg aaaaaagagg gaagccccgg atgcatatct gatctacatc cggagctccc
     1021 gctgattcac acctccggcg ataccatcgc cggctttaca cgaccgcggc tatgttcccg
     1081 cgctcgccgt cgggttcccc gggggtttta cgttcttttg aatttcgtcc atgagccgct
     1141 tgcgctcgtc gctgttgcgg tcatcgaccg tattgtggag aacgatctgg ccgttgtcgc
     1201 cggtcagcag gatgatccgg ttgccggaat tcttaggatc ctgccgcacg tcaaccagcg
     1261 tcaggccggt cttgaagtcc accgcggcgg ggccttggcc ttgcgtgctg ccgaccatat
     1321 cgcctggcgt gacctccacc gtttgcatct gccagttgcc gttcttccag cggaagatgt
     1381 cgaacttcac ccgatcaccc agcggcgacg tgctggcagc gaacaggttt gtctccgact
     1441 tcacgctgac gcgcggcgtc cagtcgcttt cggtgctgtt gatcaggaac tgatccgcca
     1501 gcgtctgcgg cttgcaaacg ttgcgcgtct ggaacaccgg gttgcgaatg atgtagcgca
     1561 gcttatagcg gtaggtatga ccgggctgga ccgaatcatc gtgggcccag atggtcacgt
     1621 cgcccgggtt cagcgcgggc gccgcggggg cagcgccggg ttgagccggc acgggcaccg
     1681 gcgcgggcgg tggttgctgg ccgttgggca gctgcccgtt cgacagcgcg atgggatcga
     1741 atgatccggg gttgggaaca ggaccgatcg gcgggaccgg gcccggcaca ggttgaccgg
     1801 gttgcggcgg tggttggttg ggcgctgccg gaccaccacc gggaccgtac ggaaccggag
     1861 cggcttccat ctccggatca aacgagatcc gaccgcggcc gacttgcgcc agcgtgatcg
     1921 gccgttccgg atcccgcggg gcgtagctgg tcgaatcacc gccgggacca ccgcggccgc
     1981 cgggggggcc gtatggcgca gcgccaccgc cgccaccgcc ggacttcggg tgcgtttgct
     2041 gatagcgttg acgttgctcc tgccgcttgc gctcggcctc ggccttgcga taatcgtcgt
     2101 acagcttctt ctggtcgggc gtcagcgtcg cgatatcctt ggcctgaacc ttgctcgggt
     2161 cgaacgcctg gttgatctga tccggctgca gcagcgcctg tcccggaaac agccaaggat
     2221 cgccctgcgt caccgcataa aacggcggtt gaagcaggtc gatctgcgtc gtctcagccc
     2281 actgctgata cgtctgcgcc tgcgtcggat tcgcgttcac cggcggcagc ggttgcaggt
     2341 ttacgttctg cagcggcggg agcaccgtct ccgggcccca gttgtctgtc gttggatcca
     2401 gctcctgtcg caccatctcc acgcgaagga tcgtcgtggt gttcggctgc ggtgggatgt
     2461 tgcgcttggt ccattcgtcg gccaggccct tcatcgggat taggccggac acggtcaccc
     2521 aattgcgatc cgtcggcgtg ccggtgctgg ccgcgggatt aggcgaaccc ggcgctgccg
     2581 gggcgggaat cgtgacgttg gatcggcctt gcgagaactg cagattcgcc ggcgccggtg
     2641 ggaccggcag caccgtgacg ggcgcggcgg ctgctacagt cgggttcggc tgtccgggct
     2701 gcccgggctg tcccggcact acggcaacgc caggcgtacc cggctgcggt cgcgcttgca
     2761 actgctccgg cgcgatgttc gatggaccat acgcgcctgc gatcgcctgg atctcgacgt
     2821 tcttgttcgc cagcacttcc tgcaggtcct gcccgaaatc cttgacgacg aacttcggca
     2881 cttccgcggt ggacattttc cgctccaggt ccttggccgg accagcgagg atcttcggat
     2941 ccacctcgcc cggtgtcacc ggcgcgctac ccacggtggc cgtcaccggc ggcagcacga
     3001 tgtagctgta gagcatgaac aggaagaaca gccccgcgat gccaagggcg acccactcga
     3061 cgttcttctc gaggatggca gttacttttt gcatgactgg tcccgtttct cgattgtctc
     3121 aactgctgac cacggataac tgacccgctg acgaccgaca tcaaggcgtc gcgcccccga
     3181 ccaccggggc gaccggcggc acaccgatcg ccacgcgaac gcggtcgggc atccacggca
     3241 ccgtccattg ccgcatgaat agcgtttcgc acttgaggtt cagctgcacg accggattgg
     3301 cgccgtaaat gaaccccgcg ttggcgcgct cgctgctgtc caccgtgacg acgtcgatgt
     3361 tgtacacgtc gatcaaccgg ttctgcccga gcattgcgat gacgtacggc acctgcgccg
     3421 cgtccacgtt aatcgccagc ttgaactgca caacgtcgta catcgggttg gacacccgac
     3481 cggtggctga gaccgcgggg accttcggca ccggctgggt gtcaccgcct tcgaccggcg
     3541 ctgccgccgg cggcgcgccc ggagtggcgg tgttcggctg caacacatac gtgcccgggg
     3601 cgggtggaat cgtcagcttc aggaagtgct tcaccggcgc gtcgaggatg ttcttgctct
     3661 tggcgttcgc ctgcgcgatc gccgtcgcca cgtcctgctg aatccacagc gacagctgcg
     3721 cgtaccagat gtcgaacggc gccggggcgt tgttggcgat cacgttcggg ttcatcgtca
     3781 gcgcatcggg cgcgatgtag atccgcttgg tcttggcgac ctccatcttc atctcgtccg
     3841 gcagcgtcag cgagcggttg gtgaagtcct gctgaacctg cgggaggttc acaggctgcc
     3901 cgttgacggt gatcacccgc ggggcgaagt cgttgttcca gatgtcgttc ttgcgcgtgg
     3961 tgatctcgac gtcggtcggc ggcacgccgg cgttgaggat gtcgttgcgg atgttcggcg
     4021 ccttggcgtt ctgcagcttc ggttcggtct tgatcgacac cgccgggtcc atgctcagca
     4081 ccagcttgta gagatcgccg aagatgaagc ggggcgtgtc ggtcgcgggg ttgggcagcg
     4141 catcctgcac gagcaggttg tgcgggtcgc ggttgatctt gtccacgaac tcgaccatgt
     4201 gcttgctctc ttcgctgacg cgcttggtcg cttcggcgcc cttggtgatg gtctgctcgt
     4261 tggggaagat cgtcagcgga tcctgcgtgg tctgcgcggg atcggtctgc ggcagcttgc
     4321 gctccttggt gatcaggccc tggagctggc tatacatgct ggcatgttga tcagcctggg
     4381 tcttgagctg ggcatacatc ccaccgatgg gccagaacgt cgccaccact gcggcaatgg
     4441 cgatcaccgc gcagacgacg ctgaagatgt tcttcttcag gatttccagt atttgtgctg
     4501 tgttcatgac acgcgttcct tgatgcattc acttccgggg cacgagcagc tcggcgcgat
     4561 cgcgatcgtc ggttggtcaa ccgatcatgg agcggcggcc gccacgggct tcgacggcga
     4621 aatggccgcc gccggaccga ccggcgcagc gggcgccgtc ggtgcgccgg gcgc
//
LOCUS       DATIMH010000198         4317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3508835, whole genome shotgun sequence.
ACCESSION   DATIMH010000198 DATIMH010000000
VERSION     DATIMH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3508835"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VLI90_03380"
     CDS             complement(<1..263)
                     /locus_tag="VLI90_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13271.1"
                     /translation="MLAPHRKLRIIVGGMVGQFPYGGVAWDYFHYCLALHELGHNVYY
                     HEDTWCWPFDPTLGYVTDNPTHTVNFIRSFFDTHAPQLADRWH"
     gene            complement(270..869)
                     /locus_tag="VLI90_03385"
     CDS             complement(270..869)
                     /locus_tag="VLI90_03385"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV13272.1"
                     /translation="MTQLAERSNQPAPSEPTERRWTRREYYRLAEQGYFKGQRVELIE
                     GRIIQMPPQGLPHTTGVELVDRAVRKAFLDGYRFRIQMPFRAADGSDPEPDVAIVKGD
                     PRDPADGHPTSAALIVEVSDTTLCHDRRKARLYAISGVSECWILTLADSTLEVYQNPM
                     PSAGAADLPYASIRQLRREDSLAPLAAPQGIIQVADLLP"
     gene            complement(904..2745)
                     /locus_tag="VLI90_03390"
     CDS             complement(904..2745)
                     /locus_tag="VLI90_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV13273.1"
                     /translation="MRFYIRAISYFKPDTGKIVLSLIVIAAMTLCGLLQVYPFAILSD
                     SVFTGLPPKDWVYRAFLAISPSTKVGQIISLSLIVLGLRLVQELLQMVMTLLNIKIGY
                     AGLMRVRCDLFRKLQELSLAYHKSQPQGDAIYRLSYDTYGFQTILNVLVQTIIVSSIT
                     LLLMIGIMLSKSWQLTLISLSVAPLLFWTTRIYSKILTRKATEAKEVDAKLTTAIQRS
                     VATVGLVQAFGREADEYANFSTTVESSVAAYLRLHWQELIFWLFVGTIFGVGGAAIFG
                     YGGYLVYRDQYILHLGEKGMTLGTLWVFLAYLTMLYGPLKSLSGSGAQLAGGVAGARR
                     VFEVLDRDPIIKDAPDAIHLPKQPRTLALDHVVFEYRKDQPVLDDVSCTIKPGEMVAF
                     VGPSGVGKTTLLNLLPRFYDPVGGAMRLDGHDARQIKIKDLRKHIALVLQESVILPTS
                     VRENIAYGYPDATDTQVHHAAEIAGASVFIEKLPNGYAEQIDEAGQNLSGGQKQRISI
                     ARALLTEAPIIIMDEPTSALDPEHEARIVETLKRIKGQRTIILVSHRLSTVADCDQIF
                     VMDAGKIIERGTHDELLALRGAYFRMAKHQMKIEDEPRFIEQSKLPA"
     gene            complement(2977..>4317)
                     /locus_tag="VLI90_03395"
     CDS             complement(2977..>4317)
                     /locus_tag="VLI90_03395"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV13274.1"
                     /translation="PNLIPDEGSWVADCVGLSPGGPTHVMIDDRTAEHVPGCNMAYYT
                     FAAKQINGFDSQFRKAGDDVDFIWRLQNAGYTIGFAPAAQVWHYRRNTVKAYLKQQRG
                     YGEAEALLKYKHPDHFNTLGASHWRGRIYGGEEIGVRFGGDVIYHGVFGTGLFQTIYR
                     QPASMLAMMMMSIEWHLLGAFVTILGLAFTPLLWVAVFMFFVPIGFAIVAAMQVPMPK
                     HRHLFTRPLIAFLHYRQPIARGWARYSARLRHKVMKVKKEARGYKRPGGLPFDPLDSQ
                     TLRYWSHEQERVGLIKQITTEVKSAGWRMRLDSGWGEWDMEIYGSRYVKVRLTTASEH
                     HSDGMLTRVRVETSMSKFCLCLFVASLILCGLLLLHVWPFSRPAVLIPLAWWAMFLVN
                     QRRVTEPVLGLIDEVAERGGFHAIPAEPSKTTATPDVKPARVGVDFEEHEPSAA"
BASE COUNT          819 a         1319 c         1331 g          848 t
ORIGIN      
        1 tagtgccacc ggtccgccag ctgcggggcg tgcgtgtcga agaagcttct aatgaagttg
       61 accgtgtggg tcgggttgtc ggtgacgtag cccagcgtcg gatcgaacgg ccagcaccag
      121 gtgtcttcgt ggtagtaaac gttgtggccc agctcgtgca gagcgaggca gtagtggaaa
      181 taatcccacg cgacgccgcc gtaggggaac tgcccgacca tgccgccgac gatgattctg
      241 agttttcgat ggggagcgag catgggcgac tacgggagca ggtctgcgac ttgaatgatc
      301 ccctgcggcg ctgccagcgg cgcgagggaa tcttcgcgcc tcagctgtcg aatgctcgca
      361 tacggcaaat ccgctgcgcc agccgacggc atgggatttt gatagacctc gagagtcgaa
      421 tcggcgaggg tcaggatcca acactcgctc acgccgctga ttgcatagag gcgggctttg
      481 cgtcgatcgt gacaaagggt cgtgtcgctg acttcgacga tgagcgccgc cgaggtcggg
      541 tgaccatctg ctggatcgcg cggatcaccc ttgacgatcg ccacgtccgg ttccgggtcc
      601 gaaccatcgg ccgcccgaaa cggcatctga attcgaaaac gatagccatc gagaaatgct
      661 ttgcgaacgg cgcggtccac cagctccacg ccagtcgtgt gaggcagccc ttgcggcggc
      721 atctgaatga tccttccctc gatcagttcg acgcgctggc ccttgaaata tccctgctct
      781 gccagccggt aatattcccg ccgggtccag cggcgttccg tgggctccga cggcgcgggt
      841 tgattgcttc gctcggctaa ctgagtcatc gcctcaccac acaatatcgt agcaccatgt
      901 tcgtcacgcc ggaagcttcg actgttcaat gaagcgcggc tcatcctcga tcttcatctg
      961 atgcttcgcc atcctgaaat acgccccgcg gagcgcgagc agctcatcgt gcgtcccgcg
     1021 ttcgatgatc tttccggcgt ccatcacgaa gatctgatcg caatcggcga ccgtgctcaa
     1081 tcggtggctg accaggatga tcgtgcgctg gcccttgatg cgttttagcg tttcgacgat
     1141 ccgcgcttcg tgctcggggt cgagcgcgct cgtcggttcg tccatgatga tgatcggcgc
     1201 ttcggtcagc agcgcgcggg cgatgctgat gcgctgcttt tgtccgccgg agaggttctg
     1261 gccggcttcg tcgatttgtt cggcgtagcc gttgggcagc ttctcgatga acactgacgc
     1321 cccggcgatc tcggcggcgt ggtggacctg agtgtcggtg gcgtcggggt agccgtaagc
     1381 gatgttctcg cggacgctgg tcggcagaat gaccgactcc tgcagcacca gcgcgatgtg
     1441 cttgcgaagg tctttgatct tgatctggcg cgcgtcgtgg ccgtccagtc gcatggcccc
     1501 gccgacggga tcgtagaagc gcggcaacag gttcagcagc gtggtcttgc cgacgccgct
     1561 ggggccgacg aatgcgacca tctcgccggg cttgatcgtg caggagacgt cgtcaagcac
     1621 gggttgatct ttgcgatact cgaacacgac atgatcgagc gccagcgtgc gcggctgctt
     1681 gggcagatgg atcgcatcgg gcgcgtcttt gatgatcgga tcacgatcga gcacttcgaa
     1741 gacgcgccgc gcgccggcga cgccgccggc taactgggca ccgctgccgg agaggctttt
     1801 cagcgggccg tagagcatcg tgaggtatgc gaggaagacc cacagcgtgc caagcgtcat
     1861 gcctttttcg cccaggtgca gaatgtactg gtcgcggtaa acaaggtaac cgccgtaacc
     1921 gaagatggcc gccccaccga cgccgaagat cgtgccgacg aaaagccaga agatcagctc
     1981 ctgccagtgc agccgcaggt acgccgcgac ggagctttcg acggtcgtgg agaagttcgc
     2041 gtactcatcc gcctcgcgcc cgaaggcttg caccaatccc accgtggcga ccgagcgctg
     2101 aatggcggtc gtcaatttcg cgtcgacttc cttcgcttcg gtcgcttttc gcgtgagaat
     2161 cttgctgtaa atccgcgtcg tccagaacag cagcggcgcg accgacagcg agatgagcgt
     2221 cagctgccag cttttgctga gcatgatgcc gatcatcagc agcagcgtga tcgagctgac
     2281 gatgatcgtc tgcaccagca cgttcaggat cgtttgaaag ccgtaggtgt cgtacgacag
     2341 gcgatagatc gcatcgccct gtggctggga cttgtgatac gcgaggctca actcctgcag
     2401 cttgcggaac agatcgcaac gcacgcgcat caacccggcg tacccgatct tgatgttcag
     2461 cagcgtcatg accatctgca gcagttcctg cacgagccgc aggcccagca cgatcagcga
     2521 cagcgagatg atctggccga ctttggttga cggcgaaatc gcgaggaagg cgcgatacac
     2581 ccaatccttc ggcggcagac cggtgaagac cgagtcggac aggatggcga aggggtagac
     2641 ctgcagcaga ccgcacaacg tcatggcggc gatgacgatc aacgagagca cgatcttgcc
     2701 cgtgtcgggt ttgaagtagg agatggctct tatgtagaac cgcatgcgat tgttttctgg
     2761 tctttccgaa gtccgaatga cgaaatccga atccccgagt caatgacgga tcgaaactcg
     2821 aatgacgaaa cctgaattcc cgaatcaatg acgaatgacg aaagtccgaa tgacgaagaa
     2881 gactaaacgc gcgctccctc cggggcttcg tcattcgtgc ttcggcattc ggatttcatt
     2941 cgggtattcg gacttcgtca ttcgggtttc cacggttcag gcggcagacg gctcgtgctc
     3001 ttcgaaatca acacccactc gcgctggttt cacgtcgggt gtcgccgtgg tctttgaggg
     3061 ctctgccgga atcgcatgaa atccaccgcg ttccgccact tcatcgatca accccaacac
     3121 cggctcggtc acgcgccgct ggttcaccag gaacatcgcc caccaggcca gcgggatcag
     3181 cacggcgggt cggctgaacg gccagacgtg cagcagcagc aggccgcata aaatcaggct
     3241 ggcgacgaac aggcagaggc agaacttgct catcgacgtc tcgacgcgca cgcgcgtcag
     3301 catgccgtcg ctgtgatgtt cgctggcagt cgtcagtcgc accttcacgt agcgcgagcc
     3361 gtaaatctcc atgtcccact cgccccaacc gctgtcgagg cgcatccgcc agccggccga
     3421 cttcacctcg gtggtgatct gcttgatcaa cccgacccgt tcctgttcgt ggctccagta
     3481 gcgcagcgtc tggctgtcga gcggatcgaa cggcaggcca ccggggcgtt tgtagccgcg
     3541 cgcttccttc ttcaccttca tcaccttatg ccgcaggcgc gcgctgtagc gcgcccaacc
     3601 gcgcgcgatg ggctggcgat aatgcaggaa tgcgatgagc ggccgggtga acaagtggcg
     3661 gtgcttcggc atcggcacct gcatcgccgc gacgatcgcg aagccgatcg gcacgaagaa
     3721 catgaacacc gcgacccaca gcagcggcgt gaacgctaat ccgaggatcg tgacgaacgc
     3781 gccgagcagg tgccactcga tgctcatcat catcatcgcc agcatcgacg cgggctggcg
     3841 gtagatcgtt tggaacaaac ccgtgccgaa gacgccgtgg tagatcacgt cgccgccgaa
     3901 gcgcacgccg atctcttccc cgccgtaaat tcgcccgcgc cagtggctgg cgcccagcgt
     3961 gttgaagtgg tccgggtgct tgtacttcag cagcgcttcg gcttcgccgt aacctcgctg
     4021 ttgtttcaaa tatgctttga ccgtgttgcg gcgatagtgc cacacctgcg ccgccggcgc
     4081 gaaaccgatc gtgtacccgg cgttctgcaa acgccagatg aagtcgacgt catcgccggc
     4141 ttttcggaac tggctgtcga aaccgttgat ctgtttcgcg gcgaacgtgt agtacgccat
     4201 gttgcagccc ggcacgtgct cggcggtgcg gtcgtcgatc atcacgtgcg tcggcccgcc
     4261 cgggctcagg cccacgcagt cggccaccca cgatccctca tccggaatca agttcgg
//
LOCUS       DATIMH010000199         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4953419, whole genome shotgun sequence.
ACCESSION   DATIMH010000199 DATIMH010000000
VERSION     DATIMH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4953419"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VLI90_03400"
     CDS             complement(<1..694)
                     /locus_tag="VLI90_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13275.1"
                     /translation="MRFRQFAPLCGVVLLCPAAVHAAAIFTGEDRIASLEIAGGFDAT
                     KNSTLNDFSDGNQSISLTHDDGTLTATASVTAVTSYSPTQYNFNILDVSDTDLHTGRS
                     FTNNAFFDSDLGFHLDTDTAYDASGILAFTQATSTDGLGPNQSFEIRLLQNGSDIRTL
                     FLTTSGPFDFAGVLPAGDYGVHMSVIAQSGVPSGTPPTGTSDVSTSVIGSFAVPEPTG
                     LATLAITAAAATT"
     gene            complement(887..1639)
                     /locus_tag="VLI90_03405"
     CDS             complement(887..1639)
                     /locus_tag="VLI90_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13276.1"
                     /translation="MKLNWSSVRARRVVVAGAAVITAAVLSSAAPAAVITGFSGNTQP
                     SHNGTDSATVNFAVFDRTGGTAGDTFDTGLSGFDSLFVAGSSSAALDTSAKYLYLYQT
                     VANGDTNTPVFQNTVGVVQPLVSSFGSFTGTKFSTTVLGTPAGFGNTSPASVGATPVI
                     LSGQSGLTSGSISDGASSVKALYLTEVPVGGASTLWGYTSNYAPALGNTAILETTGAN
                     GTAATAVPEPTAAFGAVVAAIGLCTGRRKRRA"
BASE COUNT          466 a          696 c          786 g          434 t
ORIGIN      
        1 atgtggtggc ggcggcggcg gtgatggcca gggtcgccag gccagtgggt tccggaacgg
       61 cgaacgagcc gatcaccgaa gtggagacgt cgctggtgcc ggttggaggc gtgccggatg
      121 gaacgccgga ctgcgcgatt acgctcatgt gcacgccgta atcgcccgcg ggcagaacgc
      181 cggcgaagtc gaacgggccc gacgtcgtca ggaacaacgt gcgaatatcg ctcccgttct
      241 ggaggagcct gatctcaaag ctctggttcg gccccaggcc gtccgtgctg gtggcctggg
      301 tgaaggcgag gatcccgctg gcgtcgtagg cggtatcggt gtccaggtga aagccaagat
      361 cgctatcgaa gaacgcattg ttggtgaagc ttcggccggt gtgcaggtcg gtgtcggaca
      421 cgtccaggat gttgaagttg tactgcgtcg ggctgtacga cgtgacggcc gtcacgctgg
      481 cggtggcggt gagggtgccg tcgtcgtgcg tcagcgagat gctctggttg ccgtcgctga
      541 aatcgttcag cgtggagttc ttggtggcgt caaagccccc cgcgatttcg aggctggcga
      601 tccgatcttc acccgtaaaa atcgccgccg catgcacggc ggcggggcac agaagaacaa
      661 cgccgcataa cggagcgaac tgacgaaatc gcatctgttt ctccttctcc cgttctacaa
      721 aaaacctagc gatgtggggc agacgatatg aaatgctccc cgggttaagc ggcggcgatt
      781 ataacccaga cgaatcgcga caaaataaat gcgaccgcca ttcttgatgc gcggaagttc
      841 tcgcgcgcct gggcgcgcgc gttcgcgccc gtggtctgcg gtccattcac gcgcggcgtt
      901 ttcgcctgcc ggtgcagagg ccaatcgcgg ccaccacggc gccgaatgct gcggtgggtt
      961 ccgggacggc ggtggcggcg gtgccgttgg cgccggtggt ttccaagatg gcggtgttgc
     1021 ccaacgccgg agcgtagttg gatgtgtagc cccacagcgt gctggcgcca ccgactggca
     1081 cttcggtcaa atacaacgcc ttcacgctgg acgcgccgtc gctgatgctg ccgctggtca
     1141 gaccggactg accgctcaat ataaccggcg tcgcccccac gctggccggc gaggtgttcc
     1201 caaacccggc gggggtgccc agcacggtcg tggaaaactt cgtgccggtg aaggaaccga
     1261 aggaactcac caggggttga acaacgccga cggtgttctg gaacaccggc gtgttcgtgt
     1321 cgccgttggc gacggtctga tacaggtaaa ggtacttggc cgacgtatcg agcgcggcgc
     1381 tggagcttcc ggcgacgaac aggctgtcga acccgctcag gcccgtgtcg aacgtatcgc
     1441 ccgcggtgcc accggtgcga tcaaataccg cgaagttgac ggtggcgctg tcggtgccgt
     1501 tgtggctcgg ttgcgtgttc ccggagaatc cggtgatgac cgcggccggc gcggcagagg
     1561 acagaacagc agcggtgatg accgcagccc cagccacaac aacacggcgc gcgcggacgg
     1621 aagaccaatt cagtttcatc gcattttctc caaggaagcc ctgaggggat tgaacgtgac
     1681 acaggcgccg gccgcccttc ccagggacgg gcaccgctat gaagtatggc gactaaccat
     1741 tgcctgtcaa gaaaagatgt tttggcgcag atcggccgag tcgttgctgt agcgtctttg
     1801 cattctcaag agaaaggcaa ccgccgagtc gccgagagcg ccgagacaga cgccgagaag
     1861 acgaagaggt ggaaccgcag attgcgcggc tcgactgagc tcgccgaagt cagatttcgc
     1921 agatgaagac aaagcatttt tctctctcaa attgcagaat ctgcgcaatc tgcgcttaca
     1981 gctcttctgt agtattctcg gcggcttacc tcggcgctct cggcgcctcg gcggttgcat
     2041 ttgcgccgac gcgtcaagaa ctgattaccc ctgtcaccgc cgggcggacg aatgaaatgg
     2101 acgccgttcg agccgggaca gtttcagctc taacagacag caaagcgaaa gagtagccag
     2161 gcacccgatc gcccaagcta gtaacaatga acgcggcatg atcgccacaa gccccgcaat
     2221 gcacgcgagc agggcgatga tcgcgaggat gcggtgcatc ttcatcccaa tggtgacgag
     2281 gggcggatca atgccgcccg ttcacgccgt tcatcgcttg ctggtccacc tccacatacg
     2341 cccgggcggc gtcggcggat cagcttaggc gattcgcttc gg
//
LOCUS       DATIMH010000200         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4374424, whole genome shotgun sequence.
ACCESSION   DATIMH010000200 DATIMH010000000
VERSION     DATIMH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4374424"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1273
                     /locus_tag="VLI90_03410"
     CDS             71..1273
                     /locus_tag="VLI90_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV13277.1"
                     /translation="MLLSENVMTQQQTVEYASPATLVAPRKLEWTNLWKKLGPFIGLI
                     FVFALFGALRPKTFLTQINLQIMLLQTAVVGTAALGMTVIIISAGIDLSVGSVVALST
                     VVIAKLLNLGAPPIVAAIGGTAVGVASGFVTGNLVIGQIGRVLSVLLAVGVGAWFNDH
                     NFSLWVTWLSVAAVLVVGIVINELFLKNREFNSRLGLIPFIATLGMMGALRGVAHWQG
                     DNGTIVVLGKMWLKELLVILGKNQTWRIFPPGVWLMLVLAAAVGFVLRYTRFGRHVFA
                     IGSNEQTARLCGVNVERTKLLIYMIGVGFAGLAGVLQFSYLRMGDSTTAPGMELNIIA
                     AVVIGGASLNGGEGSVVGSIVGALIMTVVANGCTKMGLETYVQEIVTGGIIIVAVLLD
                     RLRHRAGA"
     gene            complement(1314..>2255)
                     /locus_tag="VLI90_03415"
     CDS             complement(1314..>2255)
                     /locus_tag="VLI90_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSV13278.1"
                     /translation="MQPETTPTAPPVLQELSVVEYSSLPPRPPRRPRRGPAGRWFTHI
                     ALLVACTAFVLPFLWILSTSFKNVEETMASPPRWIPHVFEWRNYSEVLRQYDFLLWTR
                     NTLIVEVLTVSGTVISSALVAYGFAKIRFKGRGVLFGLMLSTMMIPFPVIMVSMFAIF
                     RWLGDHTGTPWIGTFKPLWVPAWFGSAFSIFLLRQFFITIPDELSEAARIDGCSEFGI
                     FIRVILPLARPALTVVALLSFMATWNDFLGPLIYLQRSEQFTLALGLQNLQSQHGGAS
                     WGQLMAASVLVIMPVLILFFLAQKTFIEGIATTGMKG"
BASE COUNT          418 a          694 c          734 g          409 t
ORIGIN      
        1 gcgcgattgt tgtgtcgatg gagtaccatc gacattcgcg gcaatacggc gaatcacggg
       61 cgagacgccc gtgctacttt cagagaacgt tatgacgcag cagcagaccg ttgaatacgc
      121 ctcgcccgcg acgttggtcg ctccacgcaa gctggagtgg acgaaccttt ggaaaaagct
      181 cgggccgttc atcggtctga tcttcgtctt cgcgctgttc ggcgcgctgc ggcccaagac
      241 gttcctcacc cagatcaacc tgcagatcat gctgctgcag accgccgtcg tcggcacggc
      301 ggcgctgggc atgaccgtca tcatcatatc tgccggcatc gacttgtccg tcggctcggt
      361 cgttgcgctg agcacggtgg tgatcgcgaa gctgctgaac ctgggcgccc cgccgatcgt
      421 tgcggcgatc ggcggcaccg ccgtcggtgt cgccagcggg ttcgtcacgg ggaatttggt
      481 catcgggcag atcggccggg tgttgtcggt gctgctggcc gtgggcgtgg gggcgtggtt
      541 caacgatcat aacttttcac tgtgggtgac ctggctcagc gtcgcggccg tgctggtcgt
      601 cggcatcgtc atcaacgagc ttttcctgaa gaaccgtgag ttcaacagcc ggctcggcct
      661 gatcccgttc atcgccacgc tcggcatgat gggcgcgctc cgcggcgtcg ctcactggca
      721 gggcgacaac ggcaccatcg tcgtgctcgg caagatgtgg ctgaaggaac tgctggtcat
      781 cctcggcaag aaccaaacct ggcgcatctt cccgccgggc gtgtggttga tgctcgtgct
      841 tgccgccgcc gtggggtttg tactgcgcta cacgcgcttt ggccggcacg tcttcgcgat
      901 cggttccaac gaacagacgg cgcggttgtg tggcgtgaac gtcgagcgaa ccaagctcct
      961 gatttacatg atcggcgtcg gcttcgcggg cctcgcgggc gtgctgcagt tctcgtatct
     1021 gcgcatgggc gattcgacca cagcgccggg gatggaattg aacatcatcg ccgcggtcgt
     1081 catcggcggg gcaagtctca acggcgggga aggcagcgtg gtgggctcga tcgtcggcgc
     1141 gctgatcatg accgtcgtcg ccaacggctg cacgaagatg gggctggaaa cctacgtgca
     1201 ggaaatcgtg accggcggga tcatcatcgt cgcggtgctg ctggatcgat tacggcatcg
     1261 cgcgggcgcc tgacacacgc agaccggccc gacccgctta agcgggcggg cctctacccc
     1321 ttcatcccag tcgtcgcgat gccttcgatg aacgtcttct gcgccaggaa aaacaggatc
     1381 agcaccggca tgatcaccag cacgctggca gccatcagct gtccccacga tgcgccgccg
     1441 tgctggcttt gcaggttttg caggcccagc gccagcgtga attgttccga tcgttgcagg
     1501 taaatcagcg ggcctagaaa atcgttccac gtcgccatga acgacagcag cgcgacgacg
     1561 gtcagcgcag gcctggccag gggaaggatt acgcggatga agataccgaa ttcggagcag
     1621 ccgtcgatgc gggctgcttc gctcagctca tcggggatcg tgatgaagaa ctgccgcagc
     1681 aggaagatgc tgaaggccga cccgaaccac gcgggcaccc acagcggttt gaacgtgccg
     1741 atccacggcg tgccggtgtg atcgcccagc cagcggaaga tcgcgaacat cgacaccatg
     1801 atcaccggga acgggatcat catcgtcgag agcatcagcc caaataagac cccccggccc
     1861 ttgaagcgaa tcttcgcgaa gccatacgcg accagcgcgc tggagatgac ggtgcccgag
     1921 acagtcagta cttcgacgat cagcgtgttg cgcgtccaga gcaggaagtc atactgccgc
     1981 agcacctccg aatagttccg ccattcgaac acgtgcggaa tccaccgtgg cggcgacgcc
     2041 atcgtctcct cgacgttctt aaagctggtc gagaggatcc aaaggaacgg caggacaaac
     2101 gcggtgcagg cgacgagcag cgcgatgtgc gtgaaccagc gcccggccgg cccgcggcgc
     2161 ggccgccgcg ggggacgcgg cggcaacgag ctgtactcga cgacgctgag ctcctgcaaa
     2221 accggcggcg cggtcggcgt cgtctcaggc tgcat
//
LOCUS       DATIMH010000201         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5243155, whole genome shotgun sequence.
ACCESSION   DATIMH010000201 DATIMH010000000
VERSION     DATIMH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5243155"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VLI90_03420"
     CDS             complement(<1..587)
                     /locus_tag="VLI90_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSV13279.1"
                     /translation="METIKNYLTHPEWRRQQRWRKAIWRNLNRVGLTGVHALPMSRRW
                     VDIHRRRMPLVNLDPAMNGFRLVQISDLHYSPVVWQRYLIQYVQWVNELKPDAVVVTG
                     DLITGGYRFAHRIATILTHLHAPHGIICTLGNHDYSIYGKASKQEGDRRADYLEKCLK
                     DRGLICLRNEKLQLRRDGATTPVEIVGLDDEWSGHL"
     gene            complement(740..1051)
                     /locus_tag="VLI90_03425"
     CDS             complement(740..1051)
                     /locus_tag="VLI90_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13280.1"
                     /translation="MANRLEQLQKMLEREPNDTFLLYGLALEYKKVNELDRALEYLSR
                     VTQIDPGYCYAYHQRGLVHEMRGDTAAAKQAYQEGLAAAVKKGDAHAQEEIAAALGML
                     D"
     gene            complement(1066..2496)
                     /locus_tag="VLI90_03430"
     CDS             complement(1066..2496)
                     /locus_tag="VLI90_03430"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSV13281.1"
                     /translation="MLQANTLHKPRLLILDEDRIMCQSLSQFLRREGYEVRTSDQPED
                     AFSQMEHGQIELLLADVNMPGVRSQDFLRDIRRRFPHVVVIVITSYGSIEGAVEATKM
                     GAFDYLTKPIVDDEIRVVVEKAARQQSLLFENQTLRNQLDLRFGLENVVGHDHKMLKI
                     FDLVEAVADSRTTVLMTGESGTGKSLLARAIHHRSPRRDKPFIEVSCGALPETLLESE
                     LFGHVKGAFTGAIADKLGRFMVADGGTLFLDEINSASPAMQVKLLRVLQERAFEAVGS
                     TETKTVDVRVILASNVDLTTLVAEQKFRQDLYYRINVVTIRLPGLRERLSDIPLLAGA
                     FLRHFKKESGREIIGMTEAAMAALQRYTWPGNVRELENAVERAVVLCRRPQIDVEDLP
                     EAIQFFSPRPATVGARGETSGEFDQPMPLELALEGPERRIIEAALKRNNWNRQSTAAE
                     LDINRTTLYKKMRKYRLDLGETNMVA"
     gene            complement(2665..>3767)
                     /locus_tag="VLI90_03435"
     CDS             complement(2665..>3767)
                     /locus_tag="VLI90_03435"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV13282.1"
                     /translation="EVNGALLTNFGTITGTTDVNFGGQAQGIGTYGLVNITTGGLFQP
                     GAGSNPPQLNFASVSGIAGNGTIDNATAGGFMDLFVTGPSTSTFAGTIQNTIGLLQLF
                     LRNNASLTLLTSSQPAGGQSVNTYNGGSVISSGCTLTIGAVGALPTGGSVSNNGAFII
                     NADSTCGQLGAGGTTTINTGATLHISPSVGINSQLHIIFNGTGKLDLTNNELLTNQTP
                     AGIRALLSSGVIFSSSAGANTALGYADQGGGVVEVKYTLRGDANLDGKVDVGDLGALA
                     TAYGSSSGASWVQGDFNYDGAVNVGDLGALATNYGLSLAGGTAAAAADPSVLPLARIS
                     AQAVISPVPEPAGLALIGMAVVGALKRRSRKV"
BASE COUNT          673 a         1203 c         1132 g          759 t
ORIGIN      
        1 agatgtccgg accattcatc atcgagcccg acgatctcca ccggcgtcgt cgcgccgtct
       61 cgccgcagct gcagcttctc gttccgcagg cagatcaacc cgcgatcctt caaacatttt
      121 tcgagatagt ccgcccggcg atcgccctcc tgcttcgacg ccttgccata gatgctgtaa
      181 tcgtgattgc ccagcgtgca aatgatgcca tgcggagcgt gcaggtgcgt gaggatcgtc
      241 gcgatgcgat gggcgaagcg atatccgccg gtaatcaaat cgccggtgac gacgaccgcg
      301 tcgggcttca gttcgttcac ccattgcacg tattgaatga ggtagcgctg ccagacgacc
      361 gggctgtaat gcagatcaga aatctgcacg aggcggaagc cgttcatcgc cgggtctagg
      421 ttcaccagcg gcatccgccg gcggtggatg tcgacccagc gccggctcat cggcagcgcg
      481 tgcacgccgg tcagaccaac gcggttaagg ttccgccaga tggctttgcg ccagcgttgc
      541 tggcggcgcc attcggggtg cgtgagatag ttcttgatcg tttccatcac ggtccgggcg
      601 gtcctccgtg taatggatgg tagtcgattc aggtgatttt cggcagggaa aatcgatgca
      661 aaggcgtccc acggcagagc catgggcttt ttcggaaagc ttcttcaaca aagcccgcgg
      721 ctctgccggg ggaccgacct caatccagca tccccagcgc cgccgcgatt tcctcttgcg
      781 cgtgcgcatc ccccttcttc accgccgccg cgaggccttc ttgatacgcc tgcttcgccg
      841 cggcggtgtc accgcgcatc tcgtgcacca gcccgcgctg gtgatacgca tagcaatacc
      901 cgggatcgat ctgcgtcacc cgcgacagat actccagcgc ccgatccagc tcgttcacct
      961 tcttgtattc cagcgccagc ccatacaata aaaatgtatc gttcggctcg cgctcgagca
     1021 tcttctgcag ttgttccagt cgattcgcca tcgcacacgc tcagttcaag cgaccatgtt
     1081 ggtctcgcca aggtccaatc gatacttccg cattttcttg tagagcgtcg tgcgattgat
     1141 gtccagttcc gccgcggtgg attggcggtt ccagttgttg cgcttcagcg ccgcttcgat
     1201 gatccgccgc tcggggcctt ccagcgccag ctccagtggc atcggttggt cgaattcacc
     1261 gctcgtctcg ccgcgcgcgc caaccgtagc cggacgcggc gagaagaatt gaatcgcctc
     1321 cggcagatct tccacgtcga tctgcggacg acgacagagg accaccgcgc gctcgaccgc
     1381 gttttccaac tcgcgcacgt tgccgggcca ggtgtagcgc tgcagcgccg ccatggcggc
     1441 ctcggtcatg ccaatgatct cgcggccgct ttccttcttg aaatgccgca ggaatgcccc
     1501 ggccagcagc gggatgtcgc tgagccgctc gcgcaggccc ggcaggcgga tggtcacgac
     1561 gttgatgcgg taatacaaat cctgccggaa tttctgctcg gcgaccagcg tcgtcagatc
     1621 gacgttgctc gccaggatca cgcgcacatc gaccgtcttc gtctcggtgc tgccgacggc
     1681 ttcgaacgcg cgctcctgca gcacgcgcag cagtttcacc tgcatcgccg gcgacgcgga
     1741 attgatctcg tccaggaaca gcgtgccgcc gtcggccacc atgaatcggc ccagcttgtc
     1801 ggcgatcgcg ccggtgaacg cgcccttcac gtggccgaac aattccgatt ccagcaacgt
     1861 ctccggcagc gcgccgcagc tgacttcgat gaacggcttg tcccttcgcg gtgaacgatg
     1921 atgaatcgcc cgcgccagca gcgatttgcc ggtgccgctt tcgccggtca tgagcaccgt
     1981 cgtccggctg tcggccaccg cttcgaccag gtcgaagatc ttcagcatct tgtgatcgtg
     2041 cccgacgacg ttttccaagc cgaaacgaag atccagctgg ttgcgcaggg tctgattctc
     2101 aaacagcagc gattgctgcc gggccgcctt ttcgacgacg acccgaattt cgtcatcgac
     2161 gatcggcttg gtcagataat cgaatgcccc catcttcgtc gcttcgaccg ccccttcgat
     2221 cgatccgtaa ctggtgatga cgatgacgac gacgtgcggg aaccgccggc ggatgtcgcg
     2281 cagaaaatcc tgcgatcgca cgcccggcat gttcacgtcc gccaataaaa gctcgatctg
     2341 cccgtgctcc atctgcgaaa atgcgtcttc cggctgatcg ctggtgcgga cttcataccc
     2401 ttcacgccga agaaactgtg acagcgattg gcacatgatc cggtcttcat ccaggatcag
     2461 cagccggggt ttgtgcagcg tgttggcttg gagcatgggc agcaccgata acgcagtgga
     2521 aattacacgg gcgcgggtgt cgcttcgctg caacacgcgt gcatttccag catcggcaca
     2581 atcggatgat gtcgccacgc aacaggtcaa tttttcgcgg ccaataactg aagctgccgt
     2641 tccagtggca gccggcaacg caagtcagac cttacgcgag cgccgcttca gcgcgccgac
     2701 cactgccatg ccaatcagcg caagacccgc tggctcgggc actggagaaa tgacagcctg
     2761 agcagatatc cgggcgagcg gcagcacgga aggatcagca gcggctgcgg ccgttccgcc
     2821 ggccaggctc agtccatagt tggtggcgag cgccccaaga tcgccgacgt tgaccgcgcc
     2881 gtcgtaattg aaatcgccct gcacccagct cgctccgctg ctggaaccat atgccgttgc
     2941 cagcgcgccc aggtccccga cgtccacctt gccgtccagg ttggcgtcgc cgcgcagggt
     3001 gtacttcact tcgaccacac cgccgccctg atcggcataa cccagcgcgg tgttggcgcc
     3061 ggcgcttgag ctgaagatca caccgctgct cagcagcgcc cgaatgccgg ccggcgtctg
     3121 gttcgtcaga agctcgttgt ttgtcaggtc gagcttgccg gttccgttga agatgatgtg
     3181 gagttgactg ttgatgccaa cgctggggga gatgtgcagc gtcgcgcccg tgttgatcgt
     3241 cgtggtgccg ccagcgccga gctggccgca ggtggagtcg gcgttgatga tgaatgcgcc
     3301 attgttggaa accgatccgc cggtgggcag cgcgccgacc gcgccaatcg tcaacgtaca
     3361 tcccgatgag atgacgctgc cgccgttgta cgtgttcacc gattgtcccc cagccggctg
     3421 ggaggaggtg agcaacgtga gcgaggcgtt gtttctcagg aatagctgga gcaatccgat
     3481 cgtgttttga atcgtgccgg cgaaggtgga cgtcgacggg ccggtaacga acaggtccat
     3541 gaatccgccc gccgtcgcgt tatcgatcgt gccgttgccg gcgatcccgc tcaccgacgc
     3601 gaagttgagc tgcggcgggt tggaacccgc gcccggctga aacaatccgc cggtcgtgat
     3661 gttgaccagg ccgtaggtgc cgatgccctg ggcttggcca ccgaagttga catcggtggt
     3721 gccggtgatt gtgccgaagt tggtgagcag cgcgccgttg acttcaa
//
LOCUS       DATIMH010000202         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1777546, whole genome shotgun sequence.
ACCESSION   DATIMH010000202 DATIMH010000000
VERSION     DATIMH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777546"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VLI90_03440"
     CDS             <1..479
                     /locus_tag="VLI90_03440"
                     /inference="COORDINATES: protein motif:HMM:NF021900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HSV13283.1"
                     /translation="GSSIMPGKVNPVMSEMMLQAAAQVVGNDSTIAFAGSGLGSTFEL
                     NVMMPVMAHALLESIELLAKAARVFADRTVAGLEANRERCESLVEQSLAMCTSLAPII
                     GYDKAAEIAKESLKTGKTVRQVAAERNVLSDAELDKALDPRRMTSPQADMVGSGGG"
     gene            complement(707..1666)
                     /locus_tag="VLI90_03445"
     CDS             complement(707..1666)
                     /locus_tag="VLI90_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSV13284.1"
                     /translation="MSDFIESNENKDGVDRRGFLKCMAWAGAGLVWAMKGGVLQSQAF
                     GADATDAGAGADFTFVQVSDSHIGFNKGVYSNVASTFQLAVDRVNALAKPPGLVLHTG
                     DLTHLSKPAEFDTVSQIMRSIRTDQSFYVPGEHDYFVDNGQQYLERFGKGTRGSGWQS
                     FDFRGVHFMGLVNVANLSNGKTALGVLGEDQLAWMEKDLAAVKSSTPIVVFAHVPLWT
                     VYPQWGWGTEDSGRAMSLLKRFGSATVLNGHIHQILQKVEGNVTFHTARSVAFPQPEP
                     GKAESPGPMKNVPAEKLKSMLGLTRVDYIEHAGTLAVTDSTLE"
     gene            complement(1681..>2032)
                     /locus_tag="VLI90_03450"
     CDS             complement(1681..>2032)
                     /locus_tag="VLI90_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HSV13285.1"
                     /translation="ERMGRTVQILMAMMIGGACAAASPAPATQPAAATATVKITDFHF
                     DPESVTVAHGASVTWVNQDDVPHTATAKGDKPLFDSKALDTDEHYSFTFTTAGTYTYY
                     CKVHPHMTGTIIVK"
BASE COUNT          361 a          653 c          636 g          382 t
ORIGIN      
        1 cgggaagcag catcatgccc ggcaaagtca acccggtcat gagcgagatg atgctccagg
       61 ccgccgccca ggtcgtcggc aacgactcga cgatcgcctt cgccggctcg ggcctcgggt
      121 caacgttcga gctgaacgtc atgatgcccg tgatggccca cgcgctgctc gagtcgatcg
      181 agttgctcgc caaagccgcg cgggtcttcg ccgatcgcac ggtcgccggc ctggaggcca
      241 atcgcgagcg ctgcgaatca ctcgtcgagc aaagcctggc gatgtgcacg agccttgccc
      301 ccatcatcgg ctacgacaag gccgcggaga tcgcgaagga gagcctcaag accggcaaga
      361 ccgtgcgaca ggtggcggcg gagcgaaacg tgttgagcga cgcggagctg gacaaagcgc
      421 tggacccgcg gcgaatgaca tcgccgcagg cggatatggt cgggagcggg ggtgggtgat
      481 tctgcgcctg ctcatcctcc aacgcaggcg tactcgccgg agtcgaagga tttagctgag
      541 aagtatgcga acgatcgatc gagcgaatcg cgcccatgtc gcatccttcg cgacaagatc
      601 cttcgactgc gcacgaggac gtgcttcgct ccggatgaca ctgcggaaat gcgcgaacgc
      661 ggcatcggaa actccggtgg ttctggagtg atgcgctcgc ggccgttcac tccaacgtcg
      721 aatccgtcac cgccagggtg cccgcgtgct cgatgtagtc cacccgcgtc aatcccagca
      781 tgctcttcaa cttctccgcc gggacgttct tcatgggacc tggcgattcc gccttgccgg
      841 gttcgggctg cggaaacgcc accgagcgcg cggtatggaa cgtcacgttg ccctcgacct
      901 tctgcaggat ctggtggatg tggccgttca gcaccgtggc ggagccaaag cgcttcagca
      961 gcgacatggc ccgcccgctg tcttcggtgc cccagcccca ctgcggatag accgtccaca
     1021 acggcacgtg cgcgaagacg acgatcgggg tgctggactt caccgcggcc agatctttct
     1081 ccatccacgc cagttgatcc tcgcccagca cgcccagcgc ggtcttaccg tttgataaat
     1141 tggcgacgtt caccagtccc atgaagtgca cgccgcggaa gtcaaagctc tgccacccgc
     1201 tgccgcgcgt gcctttgccg aagcgctcga gatattgctg gccgttgtcg acaaagtaat
     1261 cgtgttcgcc ggggacgtag aacgattgat ccgtgcggat cgaccgcatg atttgcgaca
     1321 cggtgtcgaa ctccgccggc ttggacaggt gcgtgagatc gcccgtgtgc agcaccagcc
     1381 ccggcggctt agccagcgca ttgacgcgat cgaccgccaa ttgaaacgtg ctggcgacgt
     1441 tgctgtacac gcccttgttg aagccgatgt ggctgtcgct gacctggacg aacgtgaagt
     1501 ccgcgcccgc gccggcgtcg gtcgcatccg ccccgaacgc ctgcgactgc agcacgccgc
     1561 ccttcatcgc ccagaccagc cccgcgccgg cccaggccat gcacttcaaa aacccccggc
     1621 ggtccacgcc gtctttgttc tcgttgcttt cgatgaaatc agacatttgg ttgctcctgg
     1681 tcatttcacg atgatcgtgc ccgtcatgtg cgggtggact ttgcagtagt aggtgtaggt
     1741 gccggcggtg gtgaacgtga acgagtagtg ctcgtcggtg tccagcgctt tggaatcgaa
     1801 caatggctta tcgcccttgg cggtggcggt gtgcggcacg tcgtcctgat tgacccacgt
     1861 caccgatgcg ccgtgcgcga ccgtcaccga ctccggatcg aagtggaagt cggtgatctt
     1921 cacggtcgcc gtcgccgccg cgggttgcgt ggccggcgcc ggtgaagcgg cggcgcatgc
     1981 acctccgatc atcatcgcca ttaaaatttg aaccgttcgt cccatgcgtt cg
//
LOCUS       DATIMH010000203         5747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3509971, whole genome shotgun sequence.
ACCESSION   DATIMH010000203 DATIMH010000000
VERSION     DATIMH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3509971"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VLI90_03455"
     CDS             complement(<1..382)
                     /locus_tag="VLI90_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13286.1"
                     /translation="MALLISNAAAVPAVDGGRANLAPVATPSSSFVSGHESLATINDG
                     FEPRNSGDHRHGCYGNWPEHGTQWIQYEWSQPISTDGVDVYWWDDHQGVRLPKAARVL
                     YWNGSAFVPVSAMLGVEHDRYNSAA"
     gene            381..719
                     /locus_tag="VLI90_03460"
     CDS             381..719
                     /locus_tag="VLI90_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13287.1"
                     /translation="MTKTHCSNEMRIVRYPPANGERGIVHKAEKVARANAHASRSAEA
                     VETEHGAFGIGGLGCADFKLPRGFKRAITVDQQHLVELAPASAIATSGVPFPLKSAIA
                     SCDGNAPAEK"
     gene            945..4382
                     /locus_tag="VLI90_03465"
     CDS             945..4382
                     /locus_tag="VLI90_03465"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13288.1"
                     /translation="MHRDQCSAMGVSDERRTVLTNAADFDGCRLVAENGLSVRVLSGG
                     CIHAIEYGEILINQVLAGPMGGGIHRLILRIDSGDGFACHEIIGPDQASAVTSGVDRV
                     LWSRTVDGIRCECTCWVHPSGEGWFFHIEVANGLPRAVRADLLMVQDVGLATRGQVRN
                     NELFTSQYLDHAAVKHAERGYVVMTRQNLPQSPGETHPWLMQGCFPAAVGFTTDGFDF
                     FSAEHRGSGGAAALGRPVIGRLVRQYETGYIAIQSSPMGLQPGGSDTRTFFAHFLADH
                     PMPTAATDVDRLSRVSAMRADLLRAMREQVNLAPMPRHVSLFQTAELLAGEQLSADDL
                     TRWFGSERRHEEIDDGTLLSFFHGEDARHVVLKAKELSVARPHGHILRAGRGVMPDEA
                     LMSCTFYASGVFASQLTLGNTSLAKMLSGVRDPLNIVRSSGLRLFVRCDPSQPWRLLG
                     AASAFEMALQECRWIYKCDGEWLTVRCRATDAEPVITFDLQATGKPVEWLICGEIAAG
                     PAEFENSPSMSVDAEQARITIRPDPKSLLGERQPGITFRIAARAPANVGRLGGDELLF
                     EDHVSRRLPYVTMQTRPTPSFTFSFSGELDDQRAIKPLATSWQDLTAGVRLRSASQEI
                     TEQIQDALRWFARDAMIHLSCPRGLEQANGGAWGVRDVCQGPVEFLLSYGRSGIVADI
                     LRRLFAQQYHVRGDWPQWFMFPPFESIQSRHCHGDVLIWPLKALCDYLEDSDDESILR
                     ERLPYTDELTFQPTDDAATMLEHVDRVIAKIERNFLPGIALPRFGDGDWDDSLQPADA
                     ILRERMVSSWTSALMFQTLRRYANAMAHFGEGERAAKSTKIADAINEDFHRHLMPDGI
                     VAGFAVFNAGGPVEYLLHPSDTRTGLRHRLIPMSRGIISGIFSRAEAQRHLDLIHEHL
                     LFPDGARLMDRPTKYEGGRERTFRRAESAAFFGREIGLQYVHAHLRYAEALAVMGRGE
                     ELLHALRVVNPIAVTQVVPGARRRQRNCYFSSSDAVFGDRYDAAASYARLRDGDIPTD
                     GGWRIYSSGPGIYTGIVMRQMLGIRRHFDWIEFDPVLSNELNGLCCQLTDGGRQFEYR
                     FAKSTTAGAPKRVTVNGRPIDSAQTIPHPYRPGGVRCRRSEFQSRLTQPLNLVQIEI"
     gene            4513..5625
                     /locus_tag="VLI90_03470"
     CDS             4513..5625
                     /locus_tag="VLI90_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSV13289.1"
                     /translation="MTDTATEISPKKTQPTLYDVCELSGVSSATVSRVFSGRAKVSEE
                     VRKRVIDAAQQLSYEPSHAARALAGRKTNTLGAIFPEIASGFYADVLSGIDEVAAESG
                     FDVLASFVGKRRSRPEMVKRLLRQGRVDALLLLNLENSTDLKPEALDQLPIILIDREI
                     SGSNLPVIGMDNVGGAEAMIEHLFEQGHRHIAVLTGPKGNHDSEQRLLGCQRAMTRLG
                     LTLDPSLVWAGEFTTDSGALAAREFLDRRQPLPDAIFCLNDAMAIGMLSELQRAGISV
                     PGDVALAGYDNVEAAAHLCLTSVACPMRLMGQVAARWAVDLITRNERPVGHRLQVRLV
                     VRNSSAGKTRPPVLPAAAAACDAAPLRNVHRPGMTA"
BASE COUNT         1048 a         1804 c         1843 g         1052 t
ORIGIN      
        1 aggcggcgct gttgtacctg tcatgctcaa ccccgagcat cgcgctgacc ggcacgaacg
       61 cgctgccgtt ccagtagagc acgcgcgccg ccttcggcag ccgaacccct tgatggtcgt
      121 cccaccaata gacgtccacg ccatcggtgc tgatcggctg gctccattcg tactgaatcc
      181 actgcgtgcc atgctcgggc caattgccgt aacacccgtg gcgatgatcg ccgctgttgc
      241 gcggctcgaa cccgtcgttg atcgtcgcga gcgattcgtg cccggaaacg aacgaactgc
      301 tcggcgtcgc gaccggcgcc aggttcgcgc gcccgccatc caccgcgggc accgccgccg
      361 cgtttgagat gagtaaggcc atgacgaaaa cccattgcag caatgagatg cgcatcgtga
      421 gatatcctcc agccaatggt gagcgaggaa ttgtacacaa agcggagaaa gtagcacgag
      481 cgaacgctca cgcgtctcga tcggccgaag ccgttgaaac cgaacatggc gccttcggga
      541 ttgggggact gggctgcgcc gacttcaaat taccgcgggg cttcaaacgt gccatcaccg
      601 ttgaccagca gcatctggtg gagttggcgc cggcgtcggc aatcgcgaca tccggcgtgc
      661 cattcccgtt gaagtccgcc atcgcctcct gtgacggaaa cgcgccggcc gaaaaatgaa
      721 gcccgctcag ttgtggatcg gagcccgatg gaacaataag cgggaaccag cagcgggtga
      781 aacgaaatct cgcggtccgg atatgtcgaa atcataccaa tgcgaaggag cttcatctcg
      841 aacttgccgg tcgcagcgcg aggttgattc ctgtagaatc aaaatcgaaa catctctttt
      901 gatccgaacg ccgttgttcg cacagctggc cgtggagggt tgccatgcat cgtgatcagt
      961 gttccgcgat gggcgtgtcg gacgagcggc gcaccgtcct caccaatgcc gccgacttcg
     1021 atggttgccg gcttgttgcg gaaaacggac tgagcgttcg cgtgctgagc ggcggctgca
     1081 ttcatgcgat cgagtacggc gagatcctga tcaaccaggt gctcgccggg cccatgggcg
     1141 gtggaatcca ccggctgatt ctacggatcg atagtgggga tggctttgcc tgccatgaga
     1201 tcatcggccc cgatcaggcg agcgcggtga cgagcggcgt cgatcgtgtg ctctggagcc
     1261 gcaccgtcga tggaatccgt tgcgaatgca cctgctgggt gcatccgagc ggcgaggggt
     1321 ggttcttcca catcgaggtc gccaatggct tgccgcgggc ggttcgcgct gatttgctga
     1381 tggttcagga cgtgggcttg gcgacgcgcg ggcaggtgcg gaacaacgag ttgttcacgt
     1441 cgcagtacct cgatcacgcg gcggtgaagc acgctgagcg cggatatgtc gtgatgaccc
     1501 ggcagaatct tccgcaatct ccgggcgaga cgcacccgtg gttgatgcag ggatgtttcc
     1561 ccgcggcggt gggattcacg acggacgggt ttgatttttt ctcagcagag catcgcggca
     1621 gcggtggggc ggcggcgctg ggcaggccgg tcattggccg actcgtgcgg caatatgaga
     1681 ccggctatat cgcgatacaa tccagcccga tgggactgca acctggtgga tctgacacgc
     1741 gaacattctt cgcgcacttt ctggccgacc accccatgcc gacggcagcg accgatgtcg
     1801 atcggctaag ccgcgtgagc gcgatgcgag cggacctgct gcgcgccatg cgcgagcaag
     1861 tgaacctggc gcccatgccg cgccacgtca gcttgttcca aaccgctgag ctcttggccg
     1921 gggagcagtt gagcgcagac gatctaaccc gatggttcgg ctccgagcga cggcatgagg
     1981 aaatcgacga cggtacgctg ctgtcattct tccatggcga agacgcacgc catgtcgtgc
     2041 tcaaggccaa ggaactatcg gtcgcgcgcc cgcacgggca catcctgcgc gccggacgcg
     2101 gcgtcatgcc agatgaggcg ctgatgtcct gcacgttcta cgccagcggc gtgttcgcct
     2161 cacaactgac gctcggcaac accagcctcg cgaaaatgct atccggcgtg cgcgacccgc
     2221 tgaacatcgt gcgatccagc ggcctgcggc tgttcgtccg ctgcgatccg tcgcagccat
     2281 ggcgactgct cggcgctgcg tcggcgttcg agatggcgct gcaggagtgc cgctggattt
     2341 acaaatgcga cggcgaatgg ttgaccgtgc gctgccgcgc cacggacgcg gagccggtga
     2401 tcacgttcga tcttcaagcg actggaaagc cggtcgaatg gctgatctgc ggtgaaatcg
     2461 ccgcggggcc ggcggagttc gagaattcgc cgagcatgag cgttgacgct gagcaggcgc
     2521 ggatcacgat tcgcccggac cccaagtcgc tgctgggcga acgccagccg ggcatcacgt
     2581 ttcgaatcgc agcccgggcg cccgcgaacg tcggccgact tggtggcgac gaactgctat
     2641 tcgaagatca cgtctcacgg cgcctgccgt atgtcacgat gcaaacgcgg ccgaccccat
     2701 cgttcacttt ttcattcagc ggtgagctgg atgaccagcg ggcgatcaaa ccgctggcca
     2761 caagttggca ggacctcacc gcaggcgttc gacttcgatc ggcatcgcag gagatcaccg
     2821 agcagattca ggacgcgctg cggtggttcg cgcgcgacgc gatgatccac ctcagctgcc
     2881 cgcgcgggtt ggaacaggcc aacggcggcg cgtggggtgt gcgcgacgtg tgccagggac
     2941 cggtcgagtt tctcctcagc tatggccgca gcggaatcgt cgccgacatc cttcgccgat
     3001 tgttcgcgca gcaatatcac gttcgcggcg attggccgca gtggttcatg ttcccgccgt
     3061 tcgagtcgat ccagtcgcgg cactgccacg gcgatgtgct gatctggccg ctgaaggcgc
     3121 tgtgcgatta cctcgaggat tcggacgatg aatcgatcct gcgcgagcgg ttgccgtaca
     3181 cggacgagct gacgtttcag ccgacagacg atgcggcgac gatgctggag cacgtcgatc
     3241 gcgtgatcgc gaagatcgag cggaatttcc tgcccggcat cgcgctgccg cggtttggcg
     3301 atggtgactg ggacgattca ctgcaaccgg ccgatgcgat ccttcgcgag cggatggtca
     3361 gctcgtggac gagcgcgctg atgttccaga cgctgcggcg ctacgcgaac gcgatggctc
     3421 atttcggtga aggcgaacgc gcggccaaaa gcacgaagat agcagatgca attaacgagg
     3481 actttcaccg ccacctgatg cccgatggca tcgtcgccgg cttcgcggtc ttcaacgccg
     3541 gcgggccggt cgaatacctg ctgcacccat ccgacacgcg caccggcctt cgacatcggt
     3601 tgatcccgat gtcgcgcggc atcatcagcg gcattttttc gcgagccgag gcccagcggc
     3661 atctcgatct gattcacgag catctgctgt tccccgacgg cgcgcgattg atggaccgcc
     3721 caacgaagta tgaaggcggc cgcgagcgca cgttccgccg ggccgaatca gcggcgttct
     3781 tcgggcgcga gatcggcctg cagtacgtgc acgcgcacct gcgctacgcc gaggcgctgg
     3841 cggtcatggg tcgtggcgag gagttgctgc acgcgctgcg ggtggtcaac ccgatcgctg
     3901 tcacgcaggt ggtgcccggc gcgcggcggc ggcagcgcaa ctgctacttc agcagctccg
     3961 acgccgtgtt cggcgatcgc tatgacgccg cggcgagcta cgcccgcctg cgcgacggcg
     4021 acatacccac cgacggtggc tggcggatct attccagcgg ccccgggatt tacaccggca
     4081 ttgtgatgcg gcagatgctc ggcatccgcc gccacttcga ttggatcgag ttcgacccgg
     4141 tgctgagcaa cgagttgaac ggtctctgtt gccagttgac cgatggcggc cggcagttcg
     4201 agtaccgctt cgcgaagtcc acaaccgccg gcgcgcccaa gcgggtaacg gtcaacgggc
     4261 gccccatcga ttcagcccag accattccgc atccgtatcg ccccggcggc gtgcgctgcc
     4321 ggcggtcgga atttcagtcc cggctcaccc agcctttaaa cctcgtccaa atcgagatct
     4381 gaccgcaaac ggacccgtcg cgcggcttaa tagccggtcg ttgcaaccac cttttgtcaa
     4441 tattttcctt gacatgctta aaatggctag ccacaatgaa agcggttaca ttgcgggttt
     4501 gagcggtatt tcatgaccga cacagccacg gaaatctcgc cgaagaagac ccagccgacg
     4561 ctttatgacg tctgcgaact ctccggcgtc tcctcggcga cggtttcccg cgttttcagt
     4621 ggccgggcca aggtcagcga agaggtccgc aaacgcgtca tcgacgccgc ccagcagctt
     4681 tcatacgaac caagccacgc cgcccgggcg ctggcgggtc gcaagaccaa cacgctgggc
     4741 gcaatcttcc ccgagatcgc cagcgggttt tatgccgatg tcctgtccgg catcgacgaa
     4801 gtcgccgctg aaagcggttt cgacgtcctg gcatcgttcg tcggcaagcg ccgcagccgg
     4861 ccggagatgg tcaagcggtt gctccgtcag ggccgcgttg acgcgctgct tctgctcaat
     4921 ctggaaaaca gcaccgatct gaaacccgaa gcgcttgatc agctgccgat catcctgatc
     4981 gaccgcgaga tcagcgggtc caatctaccc gtcatcggca tggacaacgt cggtggcgcc
     5041 gaggcgatga tcgaacacct gttcgaacag ggccatcggc acatcgccgt cctcaccggc
     5101 cccaagggca atcatgattc cgaacagcgc ctgctgggtt gccagcgcgc gatgacccga
     5161 ctgggactga cgctggaccc gagcctcgtc tgggctggcg agttcaccac cgacagcggt
     5221 gcgctggccg cccgggagtt cctcgatcgt cgccagccgt tgcccgacgc catcttctgc
     5281 ctgaacgatg cgatggcgat cggcatgctc agcgaacttc agcgcgcggg catctcggtg
     5341 cccggcgatg tcgccttggc agggtacgac aacgttgaag cggccgcgca cctgtgtttg
     5401 accagcgtgg cctgtccgat gcgattgatg gggcaagtgg cggcccgatg ggccgtcgat
     5461 ctgatcaccc ggaacgaacg gccggtcggg catcgactgc aggtgcggct ggtggttcgc
     5521 aattcctcgg cgggcaagac acgcccgccg gttcttcccg ccgcggcggc cgcatgtgat
     5581 gcggcgccgc tgcggaacgt acatcgaccg ggcatgacgg cgtagcgcgc gagcgcggca
     5641 ttaacttgcc gcgcgcgtcg tgtcgacgtt cgaatgccca tgtcacctct ggcggaggaa
     5701 acaaatgttt ccgacacgaa cacgctttgg tgcgccacgc gccgatt
//
LOCUS       DATIMH010000204         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_624480, whole genome shotgun sequence.
ACCESSION   DATIMH010000204 DATIMH010000000
VERSION     DATIMH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624480"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VLI90_03475"
     CDS             <1..208
                     /locus_tag="VLI90_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13290.1"
                     /translation="TLRQQVRELAPEIAAAQRFLVEPKPATAATKAKAQPKKTVAAPA
                     PRRERPTPKPAPAAPFSSQRVAQF"
     gene            complement(287..1381)
                     /locus_tag="VLI90_03480"
     CDS             complement(287..1381)
                     /locus_tag="VLI90_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV13291.1"
                     /translation="MRKQRGFTLVELLVVIGIIALLISILLPALSKARESANTLKCSS
                     NLRSIGQGIANYIAEYRGKIPASNWYYGLQIPGPGGAGAAPATATQGYVHWSSLIFQG
                     GKHFPTSTALASTDPVYLSNSGWDMFLCPSVNNGGLPPANTFPGNNEAGIANETAGVV
                     DVQAPRLAYMLNEALAGRSRVAPNYAGATNTYYHWIPAGRVRNSGETILATEMWGIPT
                     MMTAPSNTGGGGPASNSRRSINGFSVSLSAAAGTTGVSTVDKFYATSNGTANFRPAKV
                     DDLQSNPVTFYAGQVPPTTGLDTSLDFVGRNHGAKKTGAVAGDTKARGDWDLRKSNFL
                     YLDGHVETKHVSETVYPRNQWGEQFYSLTP"
     gene            complement(1674..>2474)
                     /locus_tag="VLI90_03485"
     CDS             complement(1674..>2474)
                     /locus_tag="VLI90_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13292.1"
                     /translation="AAIDPAAVVFTILFFSIILVMRWRWSLRIGGVLMYAIGVVPPVL
                     LHVALSVPITGDWRLGLAHLPTQPIRVASLPHLSNAPTPAAVVKRPADANDDEDFTPP
                     PPTWYTLAWVFLARFFAAFLGSHGLLTHFPVLIIGVAGVFSVMHRHWPSSTKTLAIIT
                     MAGATIVILRYVCLPVDWRWAMFAVRWYVIFLPLVLFWAGAWIRKSHHPAVWAIAGVL
                     LVFSSTVAIIGATDPMPREGYDRYTAAAALRSMIRPPTSGDAAMLAGR"
BASE COUNT          464 a          759 c          820 g          431 t
ORIGIN      
        1 aaccctgcgg caacaggtcc gcgagctggc gccggagatc gcggcagcgc agcggttcct
       61 tgtcgaaccc aaaccggcga ctgctgcgac gaaggcgaag gcgcaaccga agaagaccgt
      121 tgcggcgccc gcgccgcggc gggagcgccc gactccgaaa ccggcgccgg cggcgccgtt
      181 ttcgagtcag cgcgtcgcgc agttttgagc gcaacggtag aaaataaaaa aagcccacgc
      241 gtagctatgc gtgggctttc tcttttcatt cgacgtccgt gatttgtcac ggcgtcaagc
      301 tatagaactg ctcgccccac tggttccgcg gatacaccgt ctcgctcacg tgcttggttt
      361 ccacgtgccc gtccagatag aggaagttgc tcttgcgcag atcccaatcc ccgcgggcct
      421 tggtgtcgcc ggccaccgcg ccggtctttt tcgcgccgtg atttcggccg acgaagtcca
      481 ggctcgtgtc caacccggtg gtcggtggga cttggccggc gtagaacgtc accgggttcg
      541 actgcaggtc gtccaccttc gccggtcgga agttcgccgt gccgttactg gtggcataga
      601 acttgtccac cgtgctgacg cccgtggtcc ccgcggcggc gctgaggctg acgctgaaac
      661 cgttgatcga tcggcgcgag ttgctcgccg gcccaccacc tccggtgttc gacggcgccg
      721 tcatcatcgt cggaatgccc cacatctcgg tcgccaggat cgtctcaccg ctgttgcgca
      781 cgcgcccggc ggggatccag tgatagtacg tgttggtcgc cccggcgtaa ttcggcgcca
      841 cgcggctgcg gccggcgagc gcctcgttca gcatgtacgc caagcgcggc gcctgcacgt
      901 ccaccacacc cgcggtttca ttcgcgatgc cggcttcgtt gttccccggg aacgtgttgg
      961 ccggcgggag cccgccgttg ttgacgctgg ggcacaggaa catgtcccag ccgctgttgc
     1021 tcaggtagac cggatccgtc gaagccagcg cggttgacgt cgggaaatgt ttcccgccct
     1081 ggaagatcaa gctgctccag tggacgtagc cttgcgtcgc ggtggcgggg gcagcgccgg
     1141 cgccacctgg accggggatc tgcaaaccat agtaccagtt ggacgcgggg atcttgccgc
     1201 gatactcggc aatgtagttg gcgatgccct ggccgatcga gcgcaggttg gacgagcact
     1261 tgagggtatt ggccgactcg cgcgccttgc tgagggcggg cagcaggatg ctgatgagta
     1321 gtgcgataat cccaataaca accaaaagct cgacgagggt gaacccacgc tgcttacgca
     1381 tgacaggact ccttgcaacc gagcggcccg gccggccgcg gatagaacag ttgggaaggc
     1441 gtgccgaggc gcctccgatc acaacggacc gaagcatagc ggacgatccg cagttgtcaa
     1501 gtattcctat agatgacgta ggagattggt cgcctgcaac acgttgcggt cggtgttgca
     1561 tcatcaatat tggattaaag ctctgataaa taaggacttg cgtaatcgat gcaacgtttt
     1621 cgggctaaag ataaaacgtt gcatttcgtt gcaaaacgtt gcatcaggat tttttaccga
     1681 ccggcgagca tcgccgcgtc gccgctcgtc ggcgggcgga tcatgctccg caacgccgcg
     1741 gcggcggtgt agcggtcgta gccttcgcgc ggcatcggat cggtcgcgcc gatgatcgcg
     1801 acggtagagc tgaagaccag cagcacgccg gcgatagccc agacggcggg gtggtggctc
     1861 ttgcggatcc acgcgccggc ccagaacagg accagcggca ggaagatcac gtaccagcgc
     1921 accgcgaaca tcgcccagcg ccaatcgacc ggtaggcaga cgtagcgcag gatgacgatc
     1981 gtcgcgccgg ccatcgtgat gatggccagc gtcttggtgc tagacggcca gtgccggtgc
     2041 atgacgctga agacgccggc cacgccaatg atcagcacgg ggaagtgcgt gagcagccca
     2101 tggctgccga gaaaagcggc gaagaagcgg gcgaggaaaa cccacgcgag cgtgtaccac
     2161 gtcggcggcg ggggggtgaa gtcttcgtca tcattcgcat ccgccgggcg tttcactaca
     2221 gctgccggtg ttggagcatt cgaaagatgc ggcagcgacg cgacgcggat tggttgtgtc
     2281 ggcaggtggg cgagtcccag ccgccagtca ccggtgatcg gcacgctcag cgccacgtgc
     2341 agcagcaccg gcgggaccac gccgatcgcg tacatcagca ccccgccgat gcgcagcgac
     2401 catcgccagc gcatcaccag gatgatcgag aagaacagga tggtgaagac gacggcggcg
     2461 gggtcgatgg cggc
//
LOCUS       DATIMH010000205         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3798824, whole genome shotgun sequence.
ACCESSION   DATIMH010000205 DATIMH010000000
VERSION     DATIMH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3798824"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1206
                     /gene="prfB"
                     /locus_tag="VLI90_03490"
                     /pseudo
     CDS             166..1206
                     /gene="prfB"
                     /locus_tag="VLI90_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            1429..2310
                     /locus_tag="VLI90_03495"
     CDS             1429..2310
                     /locus_tag="VLI90_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSV13293.1"
                     /translation="MIVDCHTHIWQSPEQLGQLDLGETHRVPRPRTTRVSPQRSTEWR
                     NVPAADPDHHLAQSTAVDKSFVLGFKSRYLRAEIPNRYVSDYVNRFPQKLIGFAGIDP
                     TERGAVEEVRIAKNELKLRGITISPANQDFHPSDSRAMDVYAEAEKLGMPILIHPVGQ
                     YTEESKLEFARPYLLDEVARTFPRLPIIIAQLGQPWIDETICLLGKHHNVFADVSGLL
                     SRTWQAYGALVSAHQNQVIDKLLFGSDFPYTSATECIEALYSINQIAQGTNLPVVPRE
                     ALRGIVERDALGVLGLG"
     gene            2387..3064
                     /locus_tag="VLI90_03500"
     CDS             2387..3064
                     /locus_tag="VLI90_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13294.1"
                     /translation="MREVARFRNQSLAFRPGLLKIPLVVALLVFVVGCGTSPPVLSVQ
                     PLQSPSALTQTFTQAYTTRDAAGDYQVVLLDDPIDRASPGDAGRPLTPARLPPVRQVL
                     QIRVLWRPVSGAKPDSPAATNASLHWYVLSGPTTAGVSMIHYAGTAFVAVVPNGPGAE
                     ITIRNGLLKVVEHDGELTDPLNIFKLSGRFDAVADDARLQQIINDTRTATAEARGGDA
                     QAKVAAP"
     gene            3061..3471
                     /locus_tag="VLI90_03505"
     CDS             3061..3471
                     /locus_tag="VLI90_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV13295.1"
                     /translation="MIVFCYEPPLPEKSPVSPTAFLQTITMKDEGRTLGVARWHSPPD
                     PTAGLVQVLDLTILPPHQRVGHGKRLLEEVMAQGREWQRRRGSKLRRVWVGIEQKSQV
                     IARAFLTSRGFHHIATVDELYRDQELMVYVKSMD"
     gene            3621..>4351
                     /locus_tag="VLI90_03510"
     CDS             3621..>4351
                     /locus_tag="VLI90_03510"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSV13296.1"
                     /translation="MRNQPLVALLALLIVAPLLRAADAPPENDPNANTLTDAEKQAGW
                     RLLFDGKSLDGWHRYTDKSHPTTDPAKPSGWTVEDGVLSRLKTAGDLITDANFDNFEL
                     VFQWKISPGGNSGVIYRATEDHHQPYETGPEYQVLDNTKHPDGKNPLTSASSCYALYA
                     PTKDLTQPVGQWNQGRIIVVGTHVEHWLNGEKVVEYEWGSDQWKDLVKNSKFSAMPDY
                     GTRKTGGVDLQDHGNNVWYRNIKIRV"
BASE COUNT          899 a         1441 c         1273 g          738 t
ORIGIN      
        1 acagcccgtt caccgtccgc gtggaaacgc cgcggccgtc cttaaccaac tttgcaaaac
       61 gcgtttcatt cgcctaccct tggctcacca tgatccacga gaacctctcc caatccatcc
      121 aagacctttc atcccggatc atcgcgatcc gggactctct ttgacttccc cactcgccag
      181 gccagacgca aggaacttga agcgaagatg ggcgagccgg ggttctggga caatccggaa
      241 tcggccaagg ggatcgtctc ggaaatgaag gccatcaagg cgatgctcga cccggtcgag
      301 gagctgctca ccggcatcga cgacgtccgg gcgatgtacg agctggccga ggaggccggc
      361 gacgccgaga cgatgaacga agccgaccgg atgctggccg atctggagaa gaagggcgag
      421 aaggtcgagc tgcaagcgct gctcgacggc aagaacgacg cccgcaacgc gttcttcacc
      481 atccaggccg gcgccggcgg aaccgaggcg caggactggt ctgagatgct tctgcggatg
      541 tatctatatt ggttcgaacg ccgcgggtgg gacgttgcgg aagtcgaccg ccagtacggc
      601 gaacaagccg gcatcaaaag cgtcaccctg cacgttaagg gtccgtacac ctttggctac
      661 ctccgggccg aagcgggtgt gcatcggctg gttcgaccca gccccttcaa cgcccagaac
      721 aaacggcaaa ccagcttctc atcggtcacc gtcgttccgg agttcgacga ggcagacacc
      781 gacctggaaa ttcccgaaga cgacctcgac ttcgtcgcgt tcgtccgcgc ctccggtccc
      841 ggcgggcaga acgtcaacaa ggtcgccagc gccgtccgca tcacccacaa acccaccggc
      901 atcaccgtca cctgctcggt tgaacgcagc cagcagcaga acaagcgatt ggcgatgaac
      961 atcctgacca gcaagctcga agcgctcgag caatccaagc gcgacgaaga gctgaagcaa
     1021 gcggtcggtg acatgaaggc caacagcttc ggcagccaga tccgcaacta cgttctggac
     1081 gaccgccggg tgaaagacgt ccgcaccggc gtcgagacca gcaacgtcga gagcgtgtta
     1141 gaccgtggcg agcttgacga gtttgtcgat gccgagcttc gccgccgcaa gcgcgagaag
     1201 aaataaccga tccgtcttaa gtcgagccac atcagcagct aacgccgttt cgcccgcgca
     1261 gaatcttagg aattttgacg cggatgggaa aatgcgtaga ctcaattgag ctatcggacc
     1321 gatccgggga aagcagggcg ctcggccgat ctttgttggc ttgcctgctc aacgtcacgg
     1381 atgatctgat cgcgccgcgc tgcgtccggt gtgttgggta tttggtttat gatcgtcgat
     1441 tgtcatacgc atatctggca gtcccccgag cagctgggcc agctggacct cggtgagacc
     1501 caccgcgtcc cccggccgcg gaccacgcgc gtgtcgcctc agcgctcgac cgaatggcgc
     1561 aacgtccccg ccgccgatcc ggaccaccat cttgcccaaa gcaccgccgt tgataagtcg
     1621 ttcgtccttg gtttcaaaag ccggtatttg cgggcggaga ttccaaaccg ctacgtctcg
     1681 gattacgtca atcgctttcc gcaaaagctg atcggcttcg ccgggatcga cccgaccgag
     1741 cggggcgccg tcgaagaggt ccgcatcgcc aagaacgaac tgaagctccg cggcatcacg
     1801 atctccccgg ctaaccaaga cttccacccg tccgacagcc gggcgatgga cgtatacgcc
     1861 gaggccgaga aactcggtat gccgatcctg atccatccgg ttgggcagta caccgaggaa
     1921 agcaagctgg aatttgcccg gccctatctg ctggacgaag tcgcccgcac gttcccccgg
     1981 ttgccgatca tcatcgcgca acttggccag ccgtggatcg atgagacgat ctgcctgctc
     2041 ggcaagcacc acaacgtttt cgctgacgtc agcggcttgc tctcacgcac ctggcaagcc
     2101 tacggcgcac tcgtatcggc ccaccaaaac caggtgatcg acaagctgct gttcggcagc
     2161 gacttcccgt acaccagcgc caccgagtgc atcgaggcgc tgtacagcat caaccagatc
     2221 gcccagggca ccaacctgcc agtggtcccg cgcgaagcgc ttcgaggcat cgtcgaacgc
     2281 gatgcgctgg gtgtcctcgg cctcggataa ttcaatcctc ccccgcttga tgtttaccaa
     2341 agcgctgtgt caacccgtaa tagcccaggc cggacggccg gggatcgtgc gggaagttgc
     2401 gcgtttccga aatcaatccc tggccttccg gcctgggcta ttaaagattc cgctcgttgt
     2461 cgcacttctt gttttcgtcg tcggctgcgg gacgtcccca cctgtcctct ccgtccagcc
     2521 gctgcaatcg ccaagtgcgc tcacgcagac cttcacccag gcgtacacca cgcgcgacgc
     2581 ggcgggggat taccaggtcg tgttgctgga tgacccaatc gaccgcgctt cgccgggtga
     2641 tgccggccgg cccctcacac ccgcccgcct gccgccggtg cggcaagtgt tgcagatccg
     2701 cgtgctctgg cggccggtgt ccggcgccaa gccagactcc cccgctgcca ccaacgcatc
     2761 gctgcattgg tacgttctca gcggccccac caccgcgggc gtgagcatga tccactacgc
     2821 cggcaccgcg ttcgtcgccg tcgtccccaa tggtcccggc gcagaaatca ccattcgcaa
     2881 cgggctgctg aaagttgtcg aacacgacgg cgagctgacc gacccgctca acatcttcaa
     2941 actcagtggc cggttcgacg ccgtcgccga tgacgctcgg ctgcagcaga ttatcaacga
     3001 cacgcgcacc gccaccgccg aagcgcgcgg cggcgacgcg caggcgaagg tcgccgcacc
     3061 atgatcgtct tctgctacga accaccgctg cccgagaagt ccccggtgtc gccgacggcg
     3121 ttccttcaga cgatcacgat gaaggacgaa ggccgcacgc tcggcgtcgc ccgctggcac
     3181 agtccacccg atccaactgc gggtttggtt caggttctag acctgacaat tcttccgccg
     3241 caccagcgag ttggccatgg taagcgattg cttgaggagg tgatggcgca aggccgcgag
     3301 tggcaacgcc gccgcggcag caagctgcgg cgggtgtggg tgggcatcga gcagaaatcg
     3361 caggtgatcg ccagggcgtt cctcacctcg cgcggcttcc atcacatcgc cacggttgac
     3421 gagctgtatc gagatcaaga gctgatggtg tacgtgaagt cgatggattg actgcacacg
     3481 ccgggtgcgg aggtactccg acgacccgga tgctttgctt caatcgcgcc cgaagaatcc
     3541 aggtcgtcgc gagtacgctc cgaacccggc gtggacggcg caggtgtagc caccatggat
     3601 cggctgtaca atcccgcccc atgcgaaatc aacctctcgt cgcactgctc gccttgctca
     3661 tcgtcgcccc gctgcttcgc gccgccgacg ccccgccgga gaatgatccg aacgccaaca
     3721 ccttaactga cgccgagaaa caagccggct ggcggttgct gttcgacggc aagagcctcg
     3781 acggctggca ccgctatacc gacaagagcc atcccaccac tgacccggcg aagcccagcg
     3841 ggtggaccgt cgaagacggt gtcctgtcgc gtctcaagac cgccggcgac ttgatcaccg
     3901 acgcaaactt cgacaacttc gaactggtct tccaatggaa gatctccccc ggcgggaaca
     3961 gcggtgtcat ttatcgcgcg accgaagacc accatcagcc gtacgaaacg gggccggagt
     4021 atcaggtgct cgacaacacg aagcacccgg acggcaagaa cccgctgacc agcgcgtctt
     4081 cgtgctacgc cctgtatgcc ccgacgaagg acctgaccca gccggtgggc caatggaacc
     4141 agggccgcat catcgtggtc ggcacgcacg ttgagcattg gctcaacggc gagaaggtcg
     4201 tcgaatatga atggggcagc gaccagtgga aggacctggt gaaaaacagc aagttcagcg
     4261 caatgcccga ctacggcacc cgcaaaaccg gcggcgtcga tctgcaggac cacggcaaca
     4321 acgtgtggta tcgcaacatc aaaatccgcg t
//
LOCUS       DATIMH010000206         4061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2933182, whole genome shotgun sequence.
ACCESSION   DATIMH010000206 DATIMH010000000
VERSION     DATIMH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2933182"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..3960
                     /locus_tag="VLI90_03515"
     CDS             430..3960
                     /locus_tag="VLI90_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HSV13297.1"
                     /translation="MQTFRARIGCWLFPALLVVFARPAFCEDLSGGAWRLWRDEQAAW
                     KDDRIFLPGEVKLDQLPVNPPTGGWQTLDDSAGVPVTLPCTVEEHFWGATTRPYTTGE
                     YRSANDDHDFANGNYLGVSWWWRTFTAPVVAPGQRLIVHIRGARLRSEVYCNGKLCGY
                     SIMTELPFDADVTAAVHPGVNQLAVRITNPGGRLDWRDSQSFKWGNYELPSSLGFGGL
                     DAGIEMETVNDVAVRDLAVLNHPDLKQVDLSAAVENTAASDFGGTVRFTIARDGATVW
                     TGTVPAKLKAGQRRDVSTTATVDNAEPWDLDHPNLYRASAELDGKDHSARETDFGFRF
                     FTATGIGDDAKLTLNGKRIVVKSSISWGFWAPSGLWPNDETADREVAAVKAFGLNCIQ
                     SHRNLSKPIVLDRFDRAGLLRYEEPGSGKFALGNRSPYSSRTAQKDVANIDTSGNGGE
                     PQSFAEKYEEQKLLAMVRRDRSHPSLIIYCIQNEIGIDLHEPRIYHLLRAMRAIDPSR
                     IALLKSGIPAANQAYMLPWDETVHHDDGTGFSGWSDQHTVGGPGTYMDSLYKSPDDFS
                     HRSTNRKEIVMWGEMLGSGTPDDHQRIVDHYRATSTSGYDRADHETILAAYDRFLDQY
                     GFRSAFPTSSALFQAVGYRSYFMWQKMIENCRISDAVDYFCVSGWESTTLENHSGLLD
                     AHRNFKADPAVIHQATEPFMLVVRPRHLVLSRGDQAIVDVHVVNEVNQRGKRRLNVNA
                     IAPDGSQVFSANDTVELTGGDVFGQLLHAGHTFPMPVEGVAKVTATLTPIDGNELPIS
                     RVSELEVIDPRGPGALPARVVVLSGDAIVSDALKESLGVTPLPASALDEQQPLDAVVV
                     AINSLSNQQINAAPADGSDDPALYQVQTTSTGGVVAAWRGLREGPCKVELFLSEPSKD
                     QPAQRLFDIALNGKTVATDLDPFVEAGGRNRAVVKTFTTDTDNGAVVLSVPNIRRGPA
                     IFAAARLTDSAGRIFRTIFRADPYTDPSGDTWAAFRAQDEGLPQPQLDAALRRVSEDG
                     TRLICWPDRATDSKAFAQALAARKILNFEGTVGTSRAAWMGSWYFVRQHPLFAGLPTD
                     CAMDWRYQIPRTSSGDEDGLLLDAPRMEVIAGYGRDHDPHVGIAACAVPYGKGRIIIY
                     CMPALKRALKGDANAPAKPVALRMLANALR"
BASE COUNT          771 a         1384 c         1212 g          694 t
ORIGIN      
        1 aataagttcg ccacaacgcc cgcggaaaac tggctacgca ctctggatat cgctaacgac
       61 gaaatcatgg aggtaaaatg gcatcgtgtc atgttgcacc gcgcttccct tttaataagc
      121 caaaagagtt gagaattgaa accttcccgc gccggtttct ccgttcgcga acacctacaa
      181 tggcggcgca ctcaacttca ccggctttcc ttcgctccca ctggcgtaca gcgcatccat
      241 cagccaactg tgcagaatcc cataccacac cgggcagccc ggttcggctt tgcccaaaag
      301 gcatccacag aattccccac cggcgtccgt tgtttctggt tgaccggcag ctatcgcacc
      361 gccccttcgc ggatggcgtg acatctatcc acctttctct tccgcggcca atcagtacaa
      421 gatgtgcccg tgcaaacctt ccgcgcgcgt atcggttgct ggttgttccc cgcgttgctt
      481 gtggtcttcg ccaggccggc gttctgtgaa gacttgtctg gcggcgcctg gcgattgtgg
      541 cgggacgagc aggccgcctg gaaagacgac aggatttttc tccccggcga agtgaagctc
      601 gaccagctgc cggtcaatcc gcccaccggt gggtggcaaa cactcgatga ctcggccggc
      661 gtccccgtca cgctcccctg caccgtcgaa gaacatttct ggggcgcgac gacgcggccg
      721 tacaccactg gcgaatatcg atccgccaac gacgaccacg acttcgccaa cggcaactac
      781 ctcggcgtct cctggtggtg gcggacgttc accgccccgg tcgtcgcgcc cggccagcgg
      841 ttgatcgtcc acatccgcgg cgcccgcctg cgcagcgagg tgtactgcaa cggcaagctg
      901 tgcggctact cgatcatgac cgagctgccc ttcgacgccg acgtgacggc cgccgtgcat
      961 ccgggcgtca accagctcgc cgtgcgcatc accaaccccg gtggccggct cgactggcgc
     1021 gacagccaaa gcttcaaatg gggcaactat gaattgccca gttccctcgg cttcggcggg
     1081 ctggatgcgg ggatcgaaat ggaaaccgtg aacgacgtcg ccgtgcgcga cctggccgtt
     1141 ctcaatcacc cggacttgaa gcaggtcgac cttagcgccg cggtcgagaa caccgccgcc
     1201 agcgatttcg gtggtacggt gcgtttcacc atcgcgcgcg acggcgcgac agtctggacc
     1261 gggactgtcc cggcgaaact gaaggccggc caacgccggg atgtttcgac aaccgccacc
     1321 gtcgacaacg ccgaaccgtg ggatctcgat caccccaatc tctatcgcgc ttcggcagaa
     1381 ctcgacggca aggaccacag cgctcgcgaa accgacttcg gctttcgctt cttcaccgcc
     1441 accggcatcg gtgacgacgc caagctcacg ctgaacggca agcgcatcgt cgtgaaatcc
     1501 tcgatttcct ggggcttctg ggctcccagc ggcctttggc ccaatgacga aaccgccgac
     1561 cgcgaagtcg ccgccgtcaa agccttcggc ctgaactgca tccagtcaca ccgcaatcta
     1621 tccaagccga tcgtgctcga tcgcttcgat cgcgccggtt tgttgcgata tgaggaaccc
     1681 ggctcaggga agttcgcgct gggcaatcgc agcccgtatt caagtcgcac ggcgcagaag
     1741 gatgtagcga acatcgacac gagcgggaac ggcggtgaac cgcagtcgtt cgccgagaag
     1801 tacgaagaac aaaagctgct ggcgatggtc cgccgcgatc ggtcgcaccc cagcctgatc
     1861 atctactgca tccagaacga gatcggcatc gacctgcacg agccgcggat ttatcacctg
     1921 ctgcgcgcga tgcgcgcgat cgacccgagc cgaatcgcgc tgctcaaatc cggcatcccc
     1981 gccgccaacc aggcgtacat gctgccgtgg gacgaaacgg ttcatcacga cgacggcacc
     2041 ggtttcagcg gctggtccga ccagcacacc gtcggcgggc ccggcacgta catggactcg
     2101 ctctacaaat cgcccgacga tttctcgcac cgctcgacca accgcaagga aatcgtcatg
     2161 tggggcgaga tgctcggcag cggcacgccg gacgatcacc agcgcatcgt cgatcactac
     2221 cgcgcgacaa gcaccagcgg ctatgaccgc gccgaccacg aaacgatcct cgccgcgtac
     2281 gaccgcttcc tcgatcaata cggcttccgc tccgccttcc ccacgtcatc ggcgctcttt
     2341 caagccgttg gctatcgcag ctatttcatg tggcagaaga tgatcgagaa ctgtcgcatc
     2401 agcgacgcgg tcgattactt ctgcgtgagc gggtgggaat ccacgacgct ggaaaatcac
     2461 tcggggctgc tcgacgcgca tcgcaatttc aaagccgatc cggcggtgat ccaccaggcg
     2521 accgagccgt tcatgctggt ggtccgcccg cggcacctgg tgttgagccg cggcgaccaa
     2581 gcgatcgtcg atgtgcatgt tgtgaacgag gtgaaccagc gcggtaagcg ccggctgaat
     2641 gtgaacgcca tcgcgccgga tggttcgcaa gtctttagcg ccaacgacac cgtcgaactg
     2701 accggtggcg atgtgttcgg tcaacttctc cacgccggcc acacgttccc gatgcccgtt
     2761 gaaggagtgg cgaaggtgac ggcgacgctc acgccgatcg acggcaatga attgccgatt
     2821 tcgcgcgtgt cggagttgga agtgatcgac ccgcgcggcc cgggcgcatt gcccgcgcgg
     2881 gtcgtcgtct tgtctggcga cgcgatcgtg agcgatgcac tcaaagaatc gctgggcgtc
     2941 acgccgctgc cggccagtgc gctcgatgaa cagcaaccgc tcgacgcggt ggtggtggcg
     3001 atcaattcgc tgagcaacca gcagatcaac gccgcccccg ccgacggcag cgatgacccc
     3061 gcgttgtacc aggtgcagac caccagcacc ggcggcgtcg tcgccgcgtg gcgcggcctg
     3121 cgcgaggggc cgtgcaaggt cgagctgttc ctgtccgaac catcgaaaga ccaacccgcc
     3181 cagcggctgt tcgacatcgc gctcaacgga aaaaccgtcg cgaccgatct cgatcccttc
     3241 gtcgaagccg gcggacgaaa tcgcgcggtc gtcaaaacgt tcacaaccga caccgacaac
     3301 ggcgcggtcg tcctgtcggt ccccaacatc cgccgcggcc ccgccatctt cgccgccgcc
     3361 cgacttaccg attccgccgg ccgaatcttc cgcaccatct tccgcgccga tccctacacc
     3421 gatccgtccg gcgacacctg ggcggccttc cgcgcccaag acgagggcct gccgcaaccg
     3481 cagctcgacg cggcgctgcg gcgcgtgagc gaagacggca cccgcctgat ctgctggccc
     3541 gatcgcgcca cggacagcaa ggcgttcgcg caggcgctgg ccgcgcgaaa gatcctcaat
     3601 ttcgaaggaa cggtcggcac ctcgcgcgcc gcgtggatgg gttcgtggta tttcgtccgc
     3661 cagcacccgc tcttcgccgg cctgcccacc gactgcgcga tggactggcg ctaccagatc
     3721 cccagaacca gcagcggcga cgaagacggc ctgctgctgg acgccccgcg gatggaagtg
     3781 atcgccggct acggccggga tcatgacccg cacgtcggca tcgccgcgtg cgccgtgccc
     3841 tacggcaagg gccggatcat catctattgc atgcccgcgc tgaagcgggc gctaaaaggc
     3901 gacgcgaacg cgccggcgaa gccggtggcg ttacgtatgc tggcgaacgc gctgcggtga
     3961 acgcgcgcgc gtcagccgcc ggagccgaag gatgtagctg gaagtgattg gactgttatt
     4021 ttgcgcgaac ggtgcgagcg tcgcttgcgg tttcgcagac c
//
LOCUS       DATIMH010000207         3255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4375708, whole genome shotgun sequence.
ACCESSION   DATIMH010000207 DATIMH010000000
VERSION     DATIMH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4375708"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..3041
                     /gene="infB"
                     /locus_tag="VLI90_03520"
     CDS             39..3041
                     /gene="infB"
                     /locus_tag="VLI90_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HSV13298.1"
                     /translation="MSLKVFQIAKELGIDSKVIVKKCKDEGIPGIETHMSPVKAGLAA
                     TIREWFTAGELKTAVESTQHIDAARIQKAPRKRGSKKAASAGDAGDSGQDSATAVAEM
                     PIEEEPAIIQEAAPSVAEEAPVDVVAEAPAVVEAPAVDEVKEPVVTPPQVEVREEQAA
                     PAAVVEAPAPVEVQPVLPKVEAPVVPEVPVKPVAPLRPAAVAPAAPAVAPAAHAPVKP
                     VAPQVPQKPVRPAVRPAGVPNVPTRPTTISPVGPQVRPAPAQVQGPKVIRVEQPDQLP
                     VPRARPRPAPGQGGGYGGGGGGGPGRGPAAQPQQPTMPTPSSAGRKSGASRGEAAPVD
                     DEEAAAKKKGRTTRRGGTGRRGDAGAQAIREWREADLVEREERLASAAGVLKERKREM
                     TKQQRVPGAPAAPSAKPTHVEITPPFTIRELSEAIGIKSSDIVKKLMATGVMATVNQN
                     IEPEAAQVIALEFGIDLVLKQRESLAAAVDRVYKELGDAAEKTGRAPVVTILGHVDHG
                     KTSLLDKIRSANVAAGEAGGITQHVGAYTVEVIGGDGKPKRVTFLDTPGHQAFTAMRA
                     RGANMTDVVVLVVAADDGVMPQTVESINHAKAAGVPIVVALNKIDKPEANANKVLGQL
                     AEQGLNPVEWGGDTEVVRTSAVTGQGIKELIEYLDYTATLKELKATPSLPARGATIEA
                     FMDPNRGVVARVLVQNGTLHVGDTVVCGPAWGRVRSITDDKGKQIQEAGPSSPVEMIG
                     LGSVPGAGDKFYAMEDANHAREIAEERAMSDREGEIAQRAKVTLENLFDTIQAGTVKE
                     LRVILKADMQGSVDVLKTLLTEELSKEVKVRLLHAAVGGVTESDVLLAEASGAIIIGF
                     SVVPDETARKLAERHGVEIRTYRIIYEITDDIRKALTGMLAPTRQDQVLGHAEVRQVI
                     KVSKVGSIAGCMVTDGVLQRNNKYRLTRDGAVVVDNLTLDSLKRFKEDVKEVRGGLEC
                     GLKLAGYDDIKVGDRLEAYKTVDIARSL"
BASE COUNT          640 a          923 c         1213 g          479 t
ORIGIN      
        1 ggaatagcgg aaggacgagg atttttcggg agtcttactt gagtttgaaa gttttccaaa
       61 tcgccaaaga acttggcatc gacagcaagg tgatcgtcaa gaaatgcaag gatgaaggca
      121 ttcccggcat tgagacgcac atgagcccgg tgaaggccgg gctggcggcg acgattcgcg
      181 agtggttcac ggccggtgag ctgaagaccg cggtcgagtc gacgcagcat attgacgctg
      241 cgcgcattca gaaggcgccg cgcaagcgcg gttccaagaa ggcggcatcg gccggcgacg
      301 cgggggattc gggccaggat agcgccacgg ccgtggcgga aatgcccatc gaagaagagc
      361 ctgcgatcat tcaggaggct gcgccgtcgg tggcggaaga agccccggtg gacgtcgtgg
      421 ctgaagcgcc ggcggttgtt gaggcgccgg cggttgacga agtgaaagaa ccagtagtta
      481 ctccgcctca ggttgaggtg cgggaagagc aggctgcgcc ggccgcggtc gtagaagcgc
      541 ccgcgccggt cgaggtgcag cccgtgctgc cgaaggtgga ggctccggtt gtgccggagg
      601 tgccggtgaa gccggtggct ccgttgcgtc cggcggctgt ggccccggcc gctcccgcgg
      661 ttgcgccggc ggctcatgcg ccggtgaagc cggtggcgcc gcaggttccg cagaagccgg
      721 ttcgtccggc agtccgtccg gcgggggttc cgaatgtgcc gacgcgtccg acgacgattt
      781 cgccggtggg cccgcaggtg cgtcccgctc ccgcgcaggt gcagggaccg aaggttattc
      841 gcgtggaaca gccggaccag ttgccggtgc cccgtgcgcg gccgcggccg gcgccgggcc
      901 agggcggcgg ttatggcggg ggaggcggcg gcggcccggg tcgtggacca gcggcgcagc
      961 cgcagcagcc gacaatgccg acaccgtcga gcgcgggtcg caagagcggc gccagccgcg
     1021 gcgaggccgc gccggtggat gatgaagagg cagcagccaa gaaaaagggc cggacaacgc
     1081 ggcgcggcgg cacgggccgg cgcggggacg cgggtgcgca ggcgatccgc gagtggcgcg
     1141 aggcggattt ggtggagcgc gaggagcgtc ttgcgagtgc cgcgggcgtg ttgaaggaac
     1201 gcaagcgcga gatgaccaag cagcagcgcg tgcccggcgc gccggcggcg cccagcgcca
     1261 agccgacgca cgtggagatc acaccgccgt tcacgattcg cgaactgtcg gaagcgatcg
     1321 gcatcaagtc cagcgacatc gtgaagaagc tcatggccac gggcgtgatg gcgaccgtga
     1381 atcagaatat cgagccggag gccgcccagg tcattgcgct ggagtttggc attgatctgg
     1441 tgctgaagca gcgcgagtct ctcgcggccg cggttgatcg tgtctacaag gaactgggtg
     1501 atgccgccga aaagaccggc cgcgccccgg tggtgacgat cctcggccac gtcgaccacg
     1561 gcaagacgag cttgctcgac aagatccgct cggcgaatgt cgctgcgggc gaagcgggcg
     1621 ggattaccca gcacgtgggc gcgtatacgg tcgaagtgat cggcggcgac ggcaagccga
     1681 agcgcgtgac attcctcgac acgccgggcc accaggcgtt cacggccatg cgcgcccgcg
     1741 gggcgaacat gacggacgtg gtggtgctgg tggtggcggc ggatgacggc gtgatgccgc
     1801 agacggtcga atcgatcaac cacgcaaagg ccgccggcgt gccgatcgtg gtggcgctca
     1861 acaagatcga caagcccgaa gcgaatgcca acaaggtgct gggccagttg gccgagcagg
     1921 ggctgaatcc cgtggaatgg ggcggcgaca ccgaagtggt gcgcaccagc gcggtgacgg
     1981 gccagggcat caaagaattg atcgagtacc tcgattacac cgcgacgctg aaggagctga
     2041 aggccacgcc gagcctgccg gcgcgcgggg cgacgattga agcgtttatg gatcccaacc
     2101 gcggcgtggt ggcgcgcgtg ctggtgcaaa atggcacgct gcacgtgggc gacacggtcg
     2161 tatgcgggcc cgcgtggggc cgcgtgcggt cgatcacgga cgacaagggc aagcagattc
     2221 aggaagccgg gccatcatcg ccggtggaaa tgatcgggct gggctcggtg ccgggcgcgg
     2281 gcgacaaatt ctatgcgatg gaagacgcga accacgcgcg ggaaatcgcc gaggaacggg
     2341 cgatgagcga ccgcgagggc gagatcgccc agcgggccaa ggtgacgctg gaaaacctgt
     2401 tcgacacgat ccaggccggc acggtgaaag aactccgcgt catcctgaag gcggacatgc
     2461 agggttctgt ggacgtgctc aagacactgt tgaccgaaga gttgtcgaag gaagtgaaag
     2521 tgcggctgct gcatgcggcg gtcggcggcg tgacggagag cgatgtgctt ctggcggaag
     2581 cgtcgggcgc gatcatcatc gggttctcgg tcgtgccgga cgaaacggcg cggaaactcg
     2641 ccgagcgcca tggcgtggaa atccgcacgt accggatcat ctatgaaatc acggacgaca
     2701 tccgcaaggc cctcaccggc atgttggccc cgacgcggca ggaccaggtg ctgggccacg
     2761 ccgaggtgcg gcaggtcatc aaggtctcca aggtcggcag catcgccggc tgcatggtga
     2821 ccgacggcgt gctgcagcgg aacaacaagt accggctgac gcgcgacggc gcggtggtcg
     2881 tggacaatct gacgctggac agcctgaagc gcttcaagga agacgtgaag gaagtccgcg
     2941 gcggtttgga atgcggcttg aaactggccg ggtatgacga catcaaggtc ggcgaccgtc
     3001 tggaggccta taagacggtt gatatcgccc ggtcgctcta acgagttgtc agtggccagt
     3061 ggtcagttgt cagaaaattc catatttctg ccaacgggca actgaccact gaccactgac
     3121 gaacatgctc attggaattt tacagttgga actgcggatc ggagatgccc tgaacctcaa
     3181 ggacaaacag cggattcttc gctcgctgaa agaccggtgg catcaccacc acaacgtgtc
     3241 ggtggccgag gtcga
//
LOCUS       DATIMH010000208         3947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3511220, whole genome shotgun sequence.
ACCESSION   DATIMH010000208 DATIMH010000000
VERSION     DATIMH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511220"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1069)
                     /locus_tag="VLI90_03525"
     CDS             complement(50..1069)
                     /locus_tag="VLI90_03525"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSV13299.1"
                     /translation="MSNHRIHAALLLFLLVALGGCATDNSVINQAAAANQQLQPAIMN
                     DAQLANYIQTLGDRIVAAGKRADQAGMGPSGHTKEDSAWMFSNKMQFHIVNSKTVNAF
                     TTGGEHMYVYNALFQMCTSEDELAAVMSHEFAHVYCRHVQAGTNRQYAQLGAAGAAAV
                     AGYAVGGKENGATYASTAGSGTLALAKYFGMGYTRHDEAQADEWGFIFYCVAGWDPDH
                     FGDFFQAMIDAGYDKTPTEQSDHPMLKDRVAAAKQRAAEWKQKHDDQFHQPPIADQAT
                     FAQLKQRAAQIAASTPDDASLQKAQGLLASFSSCVSPADTQPEQVRVRQRAEEAMQKQ
                     QQQSQ"
     gene            complement(1124..2386)
                     /locus_tag="VLI90_03530"
     CDS             complement(1124..2386)
                     /locus_tag="VLI90_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV13300.1"
                     /translation="MLISVADNIERPERHDFYRGVRQFYFVEALASHAANLLLTGIFF
                     YTHQQYQWGVRQNLTLAAGQGAVYVVGALMAGRLAAQLGRRNVLLGLQVIMMLLAVQA
                     WLTPRPLTVVLVLMVYTALSSTAWPALESLVSQRASGQVLAKRLAMYNLVWSGTNALT
                     LAISGSIIQHWPGGIFILVAAAHLFAMGILLRARDRVSTIAKSEPAAAAHLEPEPALL
                     RVRTQALWLSRIALPSTYVVVYSLSAMLPSLAVMQPLPTQWRTIVGSVWFVARFATFA
                     VLGATTFWHTRPRLMLAAAAVMLVAFIGTTERPSDWFTAAHVPRAIDVLSMISWQILL
                     GAAMGIIYSASLYFGMVLSAGSTEHGGYHEALIGFGSFIGPGSAVAADLLFPGRIGAG
                     ISAVAGVIGLSLIAAAAAAAFRADDSDG"
     gene            2676..3068
                     /locus_tag="VLI90_03535"
     CDS             2676..3068
                     /locus_tag="VLI90_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13301.1"
                     /translation="MHHDPFSTTTAAEPIQPHVDRWVNEQMFEEDVRAVLRMMRPHGR
                     ERLQGWKEYRTGGAHFRVTVSWDDEFEITAPNDPLAVPHIADVIETLLVGSAKRQDEL
                     TAPEEFEQYRQVLREVYGRLQEQQACYE"
     gene            3274..3498
                     /locus_tag="VLI90_03540"
     CDS             3274..3498
                     /locus_tag="VLI90_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13302.1"
                     /translation="MKRPPNKAPKLVLKPKRTRAERAAAQRFLSILKTDPTTRRVKVK
                     CVKRSVRLRAYENDLKLSIAVDRMARELAG"
     gene            3614..>3947
                     /locus_tag="VLI90_03545"
     CDS             3614..>3947
                     /locus_tag="VLI90_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13303.1"
                     /translation="MRVPIRLSAQSPIRKLLAAVAAAAVAVLIAQLLLMATRTIDDAS
                     KFPPDPAVPPPQGNFISDAFYTWFYRQRPIESRAATEVVIVAVDQKSLDYVDENQHYG
                     WPWPREFWS"
BASE COUNT          761 a         1310 c         1155 g          721 t
ORIGIN      
        1 cgcgaggccg cggcggctaa acacgcgtcg ttaaacacgt tcgaggcgat cactgcgatt
       61 gctgttgctg cttctgcatc gcttcctcgg cgcgttgccg cacgcggact tgctccggtt
      121 gggtgtccgc cgggctcacg caactggaga acgacgccaa tagcccctgc gccttttgca
      181 ggctcgcatc gtccggcgtc gacgccgcga tctgcgccgc ccgctgcttc aactgggcaa
      241 acgtcgcctg gtccgcgatc ggtggctggt ggaactggtc atcgtgcttc tgcttccact
      301 ccgccgctcg ctgctttgcc gcggccacgc ggtccttcaa catcgggtgg tcactctgct
      361 cggtgggcgt cttgtcgtaa ccggcgtcga tcatcgcctg gaagaaatcg ccaaagtgat
      421 cgggatccca cccggcgacg caatagaaga tgaatcccca ctcgtccgcc tgcgcttcat
      481 cgtgccgcgt gtaacccatc ccgaaatatt tcgccagcgc cagcgtccca ctgcccgctg
      541 tcgatgcgta ggttgccccg ttttctttcc cgcccaccgc atatcccgcc accgccgccg
      601 cccccgcggc gccgagctgc gcgtactgcc ggttcgtccc cgcctgcacg tgccggcaat
      661 aaacgtgggc gaactcgtgg ctcatcaccg ccgccagctc gtcttcgctc gtgcacatct
      721 gaaacagcgc gttgtagacg tacatatgct cgccaccagt cgtgaacgcg ttcaccgttt
      781 tgctgttcac gatgtggaac tgcatcttgt tgctgaacat ccacgcgctg tcttcctttg
      841 tatggccaga cggacccatg cccgcctgat cggcgcgctt gccagcggcg acgatgcgat
      901 cgccgagcgt ctggatgtag ttcgccagtt gcgcatcatt catgatcgcc ggctgcaatt
      961 gctgattcgc cgccgccgcc tggttgatca cgctgttgtc ggtggcgcaa ccaccgagag
     1021 cgacaagaag gaacaaaagc aacgcggcgt ggattctgtg gttcgacatg gcgattcctc
     1081 gattcgcaac tcgcaattac cccagcgggc aatagtgtaa cgcctatcca tcggaatcgt
     1141 cagcgcgaaa cgcggctgcc gcggcggcgg cgatcaagct caatccgatc acgcccgcga
     1201 cggccgaaat acccgcgccg atgcgccctg gaaacagcag atccgccgcc accgccgaac
     1261 caggaccgat gaacgaaccg aaaccgatca gcgcctcgtg atatcccccg tgctcggtgc
     1321 tgccggcgct gaggaccatg ccgaaataca agctggccga gtagatgatg cccatcgccg
     1381 ccccgagcag gatttgccag ctgatcatcg acagcacgtc aattgcacgc ggcacgtgcg
     1441 cggcggtgaa ccaatcgctg ggccgctcgg tggtgccgat gaatgccacc agcatcaccg
     1501 ccgccgccgc cagcatcagg cgcggccggg tgtgccagaa ggtggtcgca cccagcacgg
     1561 cgaacgtcgc gaagcgcgcc acgaaccaca cgctgcccac gatggtccgc cactgcgtcg
     1621 gcaacggctg catgaccgcc agcgacggca gcatggccga caaactgtag acaacaacgt
     1681 acgtcgaagg cagcgcgatc cgcgacaacc acaacgcctg cgtgcgaacg cgcagcagcg
     1741 ccggttccgg ttcgaggtgc gcggcggcgg cgggctccga cttcgcaatt gtcgaaacgc
     1801 gatcgcgcgc ccggagcaag attcccatcg cgaacagatg cgccgccgcc accaggatga
     1861 aaatcccacc cggccaatgc tggatgatcg acccgctgat cgccagcgtc agcgcgttcg
     1921 tgccggacca gaccaggttg tacatcgcca gccgcttggc cagcacctga ccgctggcgc
     1981 gctgcgagac gaggctctcc agcgccggcc aggcggtgga actgagggcg gtgtacacca
     2041 tcagcacaag caccaccgtc aacggtcgcg gcgtcaacca cgcctgcacc gccaacagca
     2101 tcatgatgac ctgcaatccc aacagcacgt tgcgccgccc caattgcgcc gccaatcgcc
     2161 cggccatcag cgccccgacg acgtacaccg cgccctgccc cgccgccagc gtcaggttct
     2221 gccgcacacc ccattggtac tgttgatgcg tgtaaaaaaa gatcccggtc agcagcaggt
     2281 tcgccgcgtg cgatgccagt gcttcgacaa aataaaactg ccgcacgccg cgatagaagt
     2341 cgtgccgctc cggtcgttcg atgttgtctg ccacggatat caacacgata gcgaatcctt
     2401 agcattgctt ggaccgctgt acaagcaccg ccgcgtgcgc gcgggaaagc ggcactctgt
     2461 caccgcgctg caaaactttc cctcttgcga tgtttttcgc gcgcgtgtgg acaccggtaa
     2521 taccgggcga tttcgcagtt cgagacgatt ggaaaagttg tcggattgaa acgggtgact
     2581 gtcgccgtga agattcgtcc gcatcacggt cgatgctctt cgtgtcacgc ctgttgaatc
     2641 ttcaaggaag gagaaattga tacatggtcc gatcaatgca ccacgatcct ttttcaacga
     2701 cgacggccgc tgaacccatt cagccgcacg tcgatcgctg ggtcaacgag cagatgttcg
     2761 aagaggacgt tcgggcggtt ttgcgcatga tgcgcccgca tggccgcgag cgcctgcaag
     2821 gctggaaaga atatcgcacc ggcggcgcgc acttccgcgt gaccgtcagt tgggatgacg
     2881 agttcgagat caccgcgccc aatgatccgc ttgcggtgcc gcacatcgcg gacgtgatcg
     2941 aaacgctgct cgtcggctcc gccaaacggc aggacgagct gacggcgccc gaagagttcg
     3001 agcaatatcg ccaggtcctg cgcgaggtgt acggccggct gcaggagcag caggcgtgtt
     3061 atgagtgaac gatgagcgat gaccggcgag gccgaaatcc ggccggcttc gtttgttcat
     3121 tattcatcgt ttgtttcacg ccgggcgtcg ggaaggacgc ccggcgttct tttttgcaac
     3181 acgcgatgct ctcacgcagc atgatcgata attctaaaaa tccacccgct tgctaatgat
     3241 ttcccaagcc tgatccggcc gatgtaaggg ttgatgaaac gaccgccgaa caaggccccg
     3301 aagttggtgc tcaagcccaa gcgcacgcgc gccgagcgag ccgccgccca gcggtttttg
     3361 tcgatcctga aaacggaccc gacgacgcgg cgagtaaaag tgaagtgcgt gaagcgctcg
     3421 gtgcggctgc gggcgtacga gaacgatctg aaactatcga tcgcggttga ccgcatggcg
     3481 cgcgaactcg cggggtgacg tccccgcgat aaacgccacg gatagcgatc cgtgggcctt
     3541 gcttttcctg ccgcacctct ctcaattcaa gggatttttc cctccaccgc ccagacgtta
     3601 cactcccctg ccgatgcggg tgccgattcg cctctctgct caaagcccca tacgcaagct
     3661 cctcgccgct gtcgcggcgg cggcggtcgc ggtgttgatc gcgcagctat tgctgatggc
     3721 gacgcgcacc attgacgacg cgagcaaatt tcccccagac cctgcggtgc caccgccgca
     3781 ggggaatttt atcagcgacg cgttctacac ctggttctac cgccagcggc cgatcgagtc
     3841 gcgcgccgcc accgaagtcg tcattgtcgc ggtggaccag aaaagcctcg actacgttga
     3901 cgaaaatcaa cactacggct ggccgtggcc gcgggaattc tggtcgc
//
LOCUS       DATIMH010000209         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1490237, whole genome shotgun sequence.
ACCESSION   DATIMH010000209 DATIMH010000000
VERSION     DATIMH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490237"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            463..1221
                     /locus_tag="VLI90_03550"
     CDS             463..1221
                     /locus_tag="VLI90_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSV13304.1"
                     /translation="MSYRDKRTNRLLSLLSDDDYELLRGHLSQVPLEYKKSLYEASLP
                     IEHVYFPINGVASLVITMADGASAEVGTIGSEGMVGLPICLGDREAPSSVYVQVPGTA
                     LRLDAGVFRKEFERSGTLHQTIVRYAHAFFNQVAQSAACAHLHQVEQRCCRWLLMTRD
                     RMPSGEFLLTHEFLGMMLAVRRTTVTDVMGGLQKAGLIRYRRGHVTILDQEGLRQRAC
                     ECYEISKREFDRLLGDTPLAPRTDKKHRLISELG"
     gene            1484..>2161
                     /locus_tag="VLI90_03555"
     CDS             1484..>2161
                     /locus_tag="VLI90_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSV13305.1"
                     /translation="MDNKLLASLPRGDFARLLPHLSTIVLQQGAVLIEAGDEVDHVYF
                     PHYGMLSLLAVLRDGKAIETATVGREGVVGAMAGLGLYKSLVRVVVQMPMAASKIAAT
                     HFRTVVATSDPVRSLAIRYNEVLLSQARITAACNALHSIEARFCRWLLQCADRAASST
                     VPLTQEFLAEMLGVRRTSVTEVASKVQNAGAITYSRGVIRIVDRAALTRMSCECYETL
                     VEQSATLS"
BASE COUNT          425 a          655 c          668 g          413 t
ORIGIN      
        1 ccgtgcggcc atccgatctg ccaattcgcg ggggcgaagg gccatctcgc aactccagca
       61 ggaacgtcaa cagtcacgtc ccgaaccaat atatggcctg atgaaggcaa tccgagtccc
      121 cttgagcctg cccaatcagg tgaattgagt cgtatgaccg acataagctt ggtaggatac
      181 cggattgcgg cacgacgttt tccggggccg ccggcgagtg agagcccgga acggactcgt
      241 tcgttgggag tgagtccgag cgttgccgcc gtctcgtccg cgatggcacg atcaagacac
      301 ccgcgaagtg ctttttgaaa ctacgcgatg aatacagcgc ccgcgccgcg acgctggaaa
      361 gcgcggagca ccgaacccgg cacccaggct cggcgacaac acctgaagtc cggccgcgaa
      421 accgggattt gatgcttcca tcatgacggg agatcaagat ccatgtcata ccgcgataaa
      481 cggaccaacc ggttgctctc gctgttgtcg gacgatgact acgaactgct gagggggcat
      541 ctgtcgcagg tgcctcttga atacaaaaaa agcttgtacg aggcatcgct cccaatcgag
      601 cacgtgtact tcccgataaa cggggtcgct tcgctggtga tcacgatggc ggatggcgcc
      661 agtgcggagg tcggtaccat cggcagtgaa ggaatggtgg gactgccgat ctgcctcggc
      721 gaccgcgaag cgccctcatc ggtttatgtg caagtgccgg gaacggcgct gcggttggat
      781 gccggcgtat tccgtaagga gttcgagcgg agcggcacgc tgcatcagac gattgtgcga
      841 tacgcccatg ccttcttcaa ccaggtcgcg cagtcggccg cttgcgccca tcttcaccag
      901 gtcgaacagc gctgttgccg ctggcttctg atgacgcgcg accgcatgcc ttcaggagag
      961 tttctgctca cgcacgaatt tctaggaatg atgctcgcag tccgccgaac gacggtcacc
     1021 gatgttatgg gtggcctgca gaaggcgggg ctgatccggt atcgccgcgg gcatgttacc
     1081 atcctcgatc aggaggggct gcggcagcgc gcctgcgagt gctacgaaat atcgaaacgg
     1141 gaattcgatc ggctgctggg cgataccccg cttgcgccga gaaccgacaa gaagcatcgc
     1201 ctgatcagcg aactcgggta gaaacgcgtg aacggtccct gtgtccgatt tcggacaccg
     1261 gattgcgagc cgaagagcgt tccgggccta attagcgcgg catttcaacg atttgcaaaa
     1321 gacgttatga cggctaccgg accgacaatg accccgccga tgcctataaa acttttccgc
     1381 aggacgcctt tcggagcgcc cttgcaatcc ctgcggtaca tcggcccgac cgaccgggcg
     1441 ggatatcgca gtgtcaatac aggtttcaaa agccggtctc cccttggaca ataagcttct
     1501 ggcgtccctt ccgcgtggcg atttcgctcg cctgcttccg cacctttcga ccattgtcct
     1561 gcaacagggc gcggtgctga tcgaggccgg cgatgaggtc gatcacgtct acttcccgca
     1621 ttatggcatg ctgtccctgc tcgccgtgct gcgtgacggc aaggctatcg agacagccac
     1681 cgtcggccgc gaaggcgtgg tcggcgcgat ggctggcctt ggtctgtaca aatcgctggt
     1741 ccgggtcgtc gtgcagatgc caatggccgc cagcaagatc gcggccacgc atttccgcac
     1801 ggtcgtcgcc accagcgatc ccgtacgcag tctcgccatc cgttacaacg aagtgctgct
     1861 gtcgcaggcg cggatcaccg ccgcgtgcaa cgcgttgcat tcgatcgagg cccgattttg
     1921 ccgctggttg ctgcaatgcg ccgatcgcgc cgcgagcagc accgtgcccc tgacgcagga
     1981 atttctcgcg gagatgctgg gggtgaggcg cacctcggtc accgaggtgg cgagcaaggt
     2041 gcagaacgcc ggcgccatca cctactcccg aggcgtgatc aggatcgtcg atcgcgccgc
     2101 gctgacgcgt atgtcgtgcg agtgctacga gacgctggtg gagcaatccg ccacattgag
     2161 c
//
LOCUS       DATIMH010000210         6435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3800278, whole genome shotgun sequence.
ACCESSION   DATIMH010000210 DATIMH010000000
VERSION     DATIMH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3800278"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..3092)
                     /locus_tag="VLI90_03560"
     CDS             complement(84..3092)
                     /locus_tag="VLI90_03560"
                     /inference="COORDINATES: protein motif:HMM:NF040048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HSV13306.1"
                     /translation="MIAYLWRQMIVIILIGLFGIFNIIAIAAHAAGAAAPSIAIDFSH
                     AGYGAGQSLPVVRNAITVRPSGGDDTELLQAAVDHVAALPADADDYRGAVLLWPGTFR
                     VEGQLLLRGDGIVLRGSRAPGQPTAIVATGHHRRTLIEMGERRRAPTTAPAGNVVDEN
                     APAGATSLTLDSLWPLSPGDRVIITRPSTAEWIADLGMKGNKGPFADQRLDWTPGSRD
                     LHWHRIVTAVDVGSNRITLDAPITTALERRYGGGAVVKVSDDALAHRLGLEDLILDSE
                     FDSANACDEEHAWIAVALDAVEDASVRNVTARHFAGSAVRVGPRARRVTVEGCRSEQP
                     VSEPGGYRRQSFLVEGQQVLVRRCTAEAGMNDFAVGLCAAGPNVFLNCSATGALGPSG
                     GFESWASGALYERVRIAGSGIRLTNDMTRAQGGGWTAGNCVVWNCSADALDVRGPDGA
                     ANVVVESAQPLYESQLRARFGQLPAATTVPTTEANIAEFHPQLEANHPAATTTTTAPL
                     QIINGRFVINGRALWGGQVNDGWWKGNANPATALDAGVALTRFVPGRVGPGLTEDLPA
                     LAAKMVANGTPFYQSVPGLWYDRRRDEHSIVERTDGNVWAPFYEMPWARSGQAKAWDG
                     LSRFDLTRFNPWYFDRVREFESLCEQRGLLLYHNLYNTHNVLEIGPHWIDYPPRPANC
                     VNENGLPEPPPLEPGNHLHVANAFYDADNEKLRALHRAFIFHSLDELAASPNVIFSLS
                     AQFAGPLKFQQFFQDTVADWQQQHAGRPVRIALVTSKDMTDAILADPVRAKQVAVIDM
                     RYWQYRPDGSLWAPHGGQNLAFREMIAKDFGKMGDAPPPTTPAQLYRQVREYRDRYPD
                     KAIVAWNGGVGPVPILMAGGAQALMRNPSAGHGQGRSVDRTPLDAFVREHLAPVLMTM
                     TPVDGVVENGTENWCLADGGRAHLLLFSPRGATLKFAVDVGDGFRGVWFEASRGETHT
                     VDDAIDARAGVTVAKPGDGNWVLFLARTSS"
     gene            3248..4753
                     /locus_tag="VLI90_03565"
     CDS             3248..4753
                     /locus_tag="VLI90_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HSV13307.1"
                     /translation="MLVRPHQCCLTVSLLATLLLAGCTVGPDYKPPKIEVAKSFGTQA
                     GPLPATTRPSSVLESKSLGVAQWWTLFNDPTLNELMADAIRSNLDLRLAEARVREARG
                     LRATITGQRYPTVNAGAGYSHQRLSQNAPPYNAFNVPGFPWEFDLYQAGFDASWEIDV
                     FGRVRREVEAANANIEASVEDRRNTLLTLLAETARNYFELRGYQRQLVIAEENLQLQQ
                     ESVELTRNRVTNGVAPELDLTRAQAQTATTASQIPELRSQLDEALTRLAVLLGTDASQ
                     LQPRLLPADVATTQPSAVPPVPPPPPVVPVGLPSDLLRRRPDIRRAERQFAASNARIG
                     AAVADLFPRFSLTGAFNLQAGDFTHLGDWSSRSFNIGPAVSWPIFQAGQLQAAVDVRN
                     EQQQQALVQYQMTVINALREVRNAMTSFGNEQDRRTQLVQAVQADQRSLDMANQLYQQ
                     GLLDFLSVLDAQRSLYQSQDSLAQSDRLVTTDLIALYKALGGGWEIESTPH"
     gene            4768..5904
                     /locus_tag="VLI90_03570"
     CDS             4768..5904
                     /locus_tag="VLI90_03570"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HSV13308.1"
                     /translation="MSERNDDDANGNGAHTATSDAPPARRTGEPAREPRRDAPRDDEH
                     DREGRARRPWYRRPVPLLVLAVVIVAAAVGGVLWWLHARQWEETDDAFIAADVAPVTT
                     KVAGMVLRVHIQLNQDVQAGDVLVDIDPRDIQARLDQSKAEVAVAQAARETAQASSAS
                     AEADVAAAQAEFERRSSDIKRYQALDPRVVSQQQLDVARAAAQSAEAQLRASQTHLAG
                     AKAAITEAQARIEQAQAATREAELQLSYTTVRAAIAGRVTQKNVQVGQYLQVGQALMA
                     LVPRDVYIIANYKETQITNMRRGQEAEIDVDAYPDHTFHGHVDSIQSGSGAAFSLLPP
                     ENATGNYVKVVQRVPVKITFDDTASDAYKRLGPGMSVTPSVKVR"
     gene            5981..>6435
                     /locus_tag="VLI90_03575"
     CDS             5981..>6435
                     /locus_tag="VLI90_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV13309.1"
                     /translation="MSAHAASFSDAPREPVRPAKSKGQPPAATPRSLAGPHNPWLVAV
                     VVSIATFMEVLDTSIANVSLTHIAGNLSAGIDESTWVLTSYLVANAVVLPISGWLANV
                     FGRKRFYMLCVALFTGSSLLCGLAPSLGWLIVFRILQGLGGGGLAPSEQS"
BASE COUNT         1121 a         2173 c         2085 g         1056 t
ORIGIN      
        1 agcgactgcc acgccgatcg cgcgagccat ccttcaccga cgcgccagcc agacccttcg
       61 accccggcga gtacgcctgc gcttcaggat gacgttcttg ccagaaacaa aacccaattc
      121 ccatccccag gcttcgcaac cgtcactcca gctcgtgcat caatcgcgtc gtccaccgtg
      181 tgtgtctccc ccctcgacgc ctcaaaccaa acgccacgaa atccatcccc cacatccacc
      241 gcgaacttca gcgtcgcccc ccgcggcgaa aacagaagca gatgcgcgcg gcccccatcc
      301 gccaagcacc agttctccgt cccattctca accaccccat ccaccggcgt catcgtcatc
      361 aacaccggcg ccagatgctc gcgcacaaac gcgtccagcg gcgtgcgatc gaccgaacgg
      421 ccttgcccgt ggcccgcgct gggatttcgc atcagcgcct gcgcgccgcc ggccatcagg
      481 atcggcaccg gtcccacgcc gccgttccac gcgacgatcg ccttgtcggg atatcgatcg
      541 cgatactcgc gaacctgccg atacagctgc gccggcgtgg tcggcggcgg ggcgtcgccc
      601 atcttgccga aatccttggc gatcatctcg cggaacgcca ggttctgtcc gccgtgcggc
      661 gcccacagcg aaccgtcggg gcgatattgc cagtagcgca tgtcgatcac cgccacctgc
      721 ttcgcgcgca ccggatcggc caggatcgca tcagtcatgt ccttgctcgt caccagcgcg
      781 atgcgcaccg gccggcccgc gtgctgctgc tgccagtcgg ccaccgtgtc ctgaaaaaac
      841 tgctggaact tcaacggtcc ggcgaactgc gcactaaggc tgaagatgac atttggcgac
      901 gccgccaact cgtccagcga atgaaagatg aacgcgcgat gcagcgcgcg cagcttctcg
      961 ttgtccgcat cgtaaaaggc gttggcgacg tgcaggtggt tgcccggttc cagcggcggc
     1021 ggctcgggca ggccgttttc gttcacgcag ttggccggcc gcgggggata gtcgatccag
     1081 tgcgggccga tctccagcac gttgtgcgtg ttgtacaggt tgtgatacag cagcaagccg
     1141 cgctgctcgc agagcgattc aaattcgcgc acgcgatcga agtaccacgg gttgaaccgc
     1201 gtcaggtcga atcggctgag cccatcccac gcctttgctt gcccgctgcg cgcccacggc
     1261 atttcgtaga acggcgccca cacgttgccg tcggtgcgct cgacgatcga atgctcgtcg
     1321 cgccggcggt cgtaccagag gccggggacc gactgataga acggcgtgcc gttcgccacc
     1381 atcttcgccg ctagcgccgg cagatcttcg gtgagaccag gaccgacccg gccgggcacg
     1441 aaccgcgtca gcgccacccc ggcgtcgagc gcggtggcgg ggttggcgtt gcccttccac
     1501 cagccgtcgt tcacctgccc gccccagagc gcgcggccgt tgatgacgaa tcggccgttg
     1561 atgatttgca gtggcgccgt ggttgtcgtt gtcgccgctg gatgatttgc ttccagctgc
     1621 ggatgaaatt ccgcaatgtt cgcctcggtt gtcggcaccg ttgtcgccgc gggcagttga
     1681 ccgaagcgtg cccgcagttg cgactcgtac aacggctgcg ccgactcgac cacgacgttc
     1741 gcggcgccgt ccggcccgcg cacgtcgagc gcatcggcgc tgcaattcca cacgacgcaa
     1801 ttcccagcgg tccatccgcc gccctgggcg cgggtcatgt cgttggtcaa ccggatgccg
     1861 ctaccggcga tgcgtacgcg ctcatacaac gcgcccgacg cccagctttc gaacccgccg
     1921 ctgggaccga gcgcgccggt cgcgctgcaa ttaagaaaca cgtttggccc cgcggcacag
     1981 aggccaacag cgaaatcgtt catgcccgcc tcggcggtgc agcggcgcac gagcacctgc
     2041 tggccttcga ccagaaaact ttgccgccga tacccgccgg gctcggagac cggctgctcg
     2101 ctccggcaac cctccactgt cacccgccgt gcccgcgggc cgacccgcac ggccgaaccc
     2161 gcgaaatgcc gcgccgtcac gttgcgcacc gatgcatcct ccacggcgtc cagcgccacc
     2221 gcgatccacg catgttcttc atcgcacgcg ttggccgaat cgaattcgct gtcgaggatc
     2281 aggtcttcca ggccgaggcg atgagccaac gcatcatcgc tgaccttcac caccgcgcca
     2341 ccgccgtagc ggcgttcgag cgcagtcgtg atcggcgcat cgagcgtgat gcgattactc
     2401 ccgacatcga cggcagtgac gatgcgatgc caatgcagat cgcgcgagcc cggcgtccag
     2461 tcgagccgct ggtctgcgaa cggccccttg ttgcccttca tgcccaggtc tgcgatccac
     2521 tcggcggtgc tgggccgggt gatgatgacg cgatcgccgg gtgaaagcgg ccagagagaa
     2581 tcgagcgtca gcgaagtcgc gcccgccggc gcgttctcat cgacgacgtt gcccgcgggc
     2641 gcggtggtcg gcgcgcggcg acgctcgccc atctcgatca acgtccgccg atggtggccg
     2701 gtcgcgacga tcgccgtcgg ctgccccggc gctcggctgc cgcgcaggac gatcccgtcg
     2761 ccgcgcagca acagctggcc ttcaactcga aaagtccccg gccataacag caccgcgccg
     2821 cgatagtcat ctgcatccgc cggcagcgct gccacgtgat ccaccgcggc ctgaagcaac
     2881 tcggtgtcgt cgccaccgct cggtcgaacc gtgatcgcgt tgcgcaccac gggcagcgat
     2941 tgcccagcgc cgtaaccggc gtgcgagaag tcgatcgcaa tcgatggcgc ggcagcccca
     3001 gccgcgtgtg ccgctattgc gatgatatta aagatcccaa ataagccgat caaaatgatc
     3061 acaatcatct gccgccagag ataggcaatc atgcctatga cgctacccgc ggaccggccg
     3121 tcaaggcaac ggacacatcc agcgccattg ataaccgcgg gactcgcgac ccgtttttct
     3181 gtccgttgcc gcgcacgcca gagcaggact accatcggac tcgtccaaaa aacgattgtc
     3241 gccgctcatg cttgttcgtc ctcaccagtg ctgtctcacg gtttcattgc ttgcgacgct
     3301 gctgctcgcc ggttgcacgg ttgggcccga ttacaaacca ccgaagatcg aagttgcgaa
     3361 aagtttcggg acgcaggcgg gaccgctgcc ggcaacgacc cggcccagtt ccgtcctcga
     3421 atcgaaatcg cttggtgtgg cgcagtggtg gacgctgttc aacgatccca cgctcaacga
     3481 gctgatggcg gacgcgattc ggtcgaacct ggacctgcgc ctggccgagg cgcgcgtgcg
     3541 cgaggcccgc ggcttgcgcg cgaccatcac cggacagcga tatccgaccg tgaacgcggg
     3601 ggcgggatat tcgcatcagc gattgagcca gaacgcgccg ccttacaacg catttaacgt
     3661 acccggattt ccgtgggagt tcgacctgta ccaggcgggt ttcgatgcat cgtgggagat
     3721 cgacgtcttc ggccgcgtgc gccgcgaagt cgaggcggcg aacgcgaaca tcgaagccag
     3781 cgtcgaggac cgccgcaaca cgctgctgac attgctcgcc gagacggcgc ggaattactt
     3841 cgagcttcgc ggctatcaac gccaactggt gatcgctgag gaaaacctgc agctgcagca
     3901 ggagagcgta gagctgacgc gcaaccgcgt gaccaacggc gtggcgccgg aactggatct
     3961 gacgcgcgcg caagcacaga cggcgacgac ggcgtcgcag attccagaac tgcgcagcca
     4021 actggacgag gcgctgacgc ggctggcggt gctgctgggc accgatgcat cgcagcttca
     4081 accgcggttg ttgccggcgg acgtggcaac gacgcagccg agcgccgtgc cgcccgtgcc
     4141 accaccaccg ccggtcgtgc cggttgggct gccgtcggat ttgctgcgtc gccggccgga
     4201 catccgccgg gccgagcggc agttcgcggc atccaacgcg cgcatcggcg ccgccgtggc
     4261 cgatctgttc ccgcggttct cgctcaccgg cgcgttcaat ctgcaggccg gcgacttcac
     4321 gcatctgggc gactggagca gccgaagctt caacatcggc ccggcagtgt cgtggccgat
     4381 ctttcaggcg ggtcaactgc aggctgccgt cgatgttcgc aacgaacagc agcagcaggc
     4441 gctggtgcag tatcagatga cggtcatcaa cgcgttgcgc gaggtgcgca acgcgatgac
     4501 gtcgttcggc aacgaacagg accggcgaac gcagctggtg caggcggtgc aggcggacca
     4561 gcgttcgctg gacatggcaa accagctgta tcagcaggga cttttggact tcctgtcggt
     4621 gctggatgcc cagcggtcgc tgtatcagtc gcaggattcg cttgcgcagt ccgaccgatt
     4681 ggtcacgact gatttgattg cgctatacaa ggcgctgggt ggcggttggg agatcgaatc
     4741 gactccgcat tgagtttggt gatcgcgatg tccgaacgaa acgacgatga cgcgaacggc
     4801 aatggcgccc acaccgcgac cagcgatgcg ccaccggcgc ggcgcaccgg cgagccggcg
     4861 cgcgagccgc ggcgggatgc gcctcgcgac gacgaacatg accgcgaagg ccgcgcgcgg
     4921 cggccatggt atcgccggcc ggtgccgctg ctggtgctgg cggtggtcat cgttgccgcg
     4981 gcggtcggtg gggtgctgtg gtggctgcac gcgcggcagt gggaggaaac cgatgacgcg
     5041 ttcatcgccg ccgacgtggc gccggtcacc accaaggtgg ccgggatggt cttgcgcgtt
     5101 cacatccaat tgaaccagga cgtgcaggcg ggcgacgtgc tggtggatat cgatccgcgc
     5161 gatatccagg cgcggctgga ccagtccaag gccgaggtgg cggtggctca ggcggcgcgc
     5221 gagaccgcgc aggcgtcgtc ggcgtcggcg gaagcggacg tcgcggcggc gcaggcggaa
     5281 ttcgagcgac ggtcatcgga catcaagcgc taccaggcgc tggatccacg cgtcgtttcg
     5341 caacagcaac tggacgtggc gcgggcggcg gcacaaagcg ctgaggcgca gttgcgcgct
     5401 tcgcagacgc acctggccgg ggccaaggcg gcgatcaccg aggcgcaagc gcggatcgag
     5461 caggcgcagg cggcgacgcg cgaggcggag cttcagctgt cgtacaccac cgtgcgtgcc
     5521 gccatagcgg gtcgcgtgac gcagaagaac gtgcaggtgg ggcagtacct gcaggtgggg
     5581 caggcgctga tggcgctggt cccgcgcgac gtgtacatca tcgccaatta caaggagacg
     5641 cagatcacca acatgcgccg cgggcaggag gcggagatcg acgtcgatgc gtatcctgat
     5701 cacacgttcc acgggcacgt ggacagcatt cagtccggca gcggggcggc gttcagcctg
     5761 ctgccgccgg agaacgcgac ggggaattac gtgaaggtcg tgcagcgcgt gccggtgaag
     5821 atcacgttcg acgacaccgc gagcgatgcc tacaagcgcc tcggaccggg aatgtcggtg
     5881 acgccgtcgg tgaaggtcag atgatggacg cgggcgggcg acaaactgcc gcagccgcag
     5941 ggcgttgccc tgcgggtatt gaaccgtcct ttgttcagtg atgtctgcac acgccgccag
     6001 tttctccgac gccccgcgtg aaccggtgcg gcccgcgaag tccaaaggcc aaccgccggc
     6061 ggcgacgcct cgttcgctcg ccgggccgca caatccgtgg ctcgtcgccg tcgtcgtttc
     6121 gatcgcgacg ttcatggagg tgctcgacac ctcgatcgcc aacgtcagcc tcacccacat
     6181 cgcgggcaat ctctcggccg gtatcgacga gagcacctgg gtgctgacga gctacctcgt
     6241 ggccaacgcg gtcgtgctgc cgatcagcgg ctggctggcg aacgtcttcg gccgcaagcg
     6301 gttttacatg ctctgcgtcg cgctgttcac cggctcgtcg ctgctgtgcg gtctcgcgcc
     6361 atcactcggc tggctgatcg tcttccgaat cctgcagggc ctcggcggcg gggggctggc
     6421 gcccagcgag caatc
//
LOCUS       DATIMH010000211         4236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_336624, whole genome shotgun sequence.
ACCESSION   DATIMH010000211 DATIMH010000000
VERSION     DATIMH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336624"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VLI90_03580"
     CDS             complement(<1..257)
                     /locus_tag="VLI90_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13310.1"
                     /translation="MKLTVSTLSCPAWELPRIVQTAQACGITGIDFRGLGAEIDITRL
                     PLFNEELDQTLAFLRAHGIELPCLNTSVTLISPAPERWSAML"
     gene            531..716
                     /locus_tag="VLI90_03585"
     CDS             531..716
                     /locus_tag="VLI90_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13311.1"
                     /translation="MLETITNNLPAIASISPITVAGVGVSALAALLYLFTRVPEMLLV
                     IATAIIYAAVPLLPRMF"
     gene            complement(828..1907)
                     /locus_tag="VLI90_03590"
     CDS             complement(828..1907)
                     /locus_tag="VLI90_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSV13312.1"
                     /translation="MSISQIAREAKVPYATAWRIINNRPCRSPEAVHAVKAAIARVGY
                     APEEIRRGRRPKSADGIRTHNVALLHLREGSSISSSVLAHVQRMLADRNLNLIFAHVE
                     SAAQLPQAIRSGNVDGILGYGEFPSDAITPQIKMVPAVWMMSRASAGTGDAWGDRVRP
                     DNQEIGRLAAQYLIDHGHQHIAYFNPDPALSMYAEREMGFRAAGEAAAASSGGERTVH
                     AFCGQPLRPAEQNSIPAMEAASERFVEKWLATSPRPTGVFIPVDRVTLRAYRHLEQHG
                     IKPGRDVQVVSCDNEQELLSLMDPRPDSIDLNRKSIAQLAVERLLWRMKHRMSAPAVT
                     ISVGPVLQRHDAPVSERIESQSAIV"
     gene            2113..2859
                     /locus_tag="VLI90_03595"
     CDS             2113..2859
                     /locus_tag="VLI90_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13313.1"
                     /translation="MSRIRTPDGIEIASLKGKKTEFFDAMAPGPGTPWEDRGTRGVVS
                     AFLKTCAMSLTSFFKLVDAIRRPETTNDAFVFLIDCCILWGVSAFIHGLIFMHFAGKV
                     QYHEVETQTYLMYVAIAAVGAGGGVYLLFRLYNIIYAKLIAQEKTATALPDVLLYNVN
                     AYALGPSILAPIPFIGPALAGLFIFIDLYIAGRKRLNIRATGALIDALMAYVVVLVVA
                     FVGYWVGNLVLHQVLGDPIDDVTPKTAQTR"
     gene            2856..3575
                     /locus_tag="VLI90_03600"
     CDS             2856..3575
                     /locus_tag="VLI90_03600"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HSV13314.1"
                     /translation="MSTSRVDRERHALLWRIHRRFPTVTATVQFGPVTFPFTRVADPD
                     RVLDEVAEEEDRRERLGGVRLEDPLHLPYWAELWDSAAGMAQYLAREPTAIASKRVLD
                     LGCGQGLAGCAAAALGANVLFADLEPPALLFAKLNSLPFSPRVRTRRLNWQRDRLDER
                     FDVILGADILYERKQWEFLEPFWRAHLSHAGSVLLGEPGRQTGDLFVEWIAQRGWWLE
                     RFEEPVPTRDRPIRIFKLRLR"
BASE COUNT          755 a         1263 c         1430 g          788 t
ORIGIN      
        1 agcatcgccg accaacgctc cggcgccggc gaaatcagcg tgaccgacgt gttgaggcac
       61 ggcagctcga tgccatgcgc gcgcaggaac gcgagcgtct ggtccagttc ctcgttgaac
      121 agcggcaatc gcgtgatgtc gatctccgcg cccagcccgc ggaagtcgat gccggtgatc
      181 ccacacgctt gcgcggtctg cacgatccgc ggcagctccc acgccgggca ggacaacgtc
      241 gatacggtca gcttcatgcc gcatgacgct agccatggca agcggaattg caaccgccga
      301 ggcgatcgcg tgtgacccat aacatcgcgt ttcgcgccgc gacctgctcg tcacgcgcgt
      361 cgcgcgcgca ataaacccaa cttgacggag gcgaaaacgt atcgaatggg cgcgctttcc
      421 cggccggcga aaaacgcggt gatagtgttg gagcacgaac gtgattgaac agcggcgcgc
      481 cgcgaggagg cgctggatca gttgcgtcgc aacactttga ggagccagcc atgctcgaga
      541 cgatcaccaa caacctgccc gcgatcgcgt cgatctcccc gatcacggtc gccggtgtcg
      601 gcgtgagcgc gctggcggcg ctgctttacc tgttcacgcg ggtgccggag atgctgctgg
      661 tgatcgccac cgcgatcatt tacgcggcgg tgccgctgtt gccgcggatg ttttgagttt
      721 gatgaccgcg ttatgcgctg cagttgtcga acattcgttc ggcgctgaac gcccgctcgg
      781 cccttcgggc cgccgctaaa aggggattcg agaatcatca gcgccgatta gacgatcgcg
      841 ctttgacttt cgattcgctc gctcactggt gcatcgtgcc gctgcagcac cgggcctacc
      901 gagatcgtca ccgcgggggc gctcatgcgg tgcttcatcc gccataacaa tcgctcgacc
      961 gccagctggg cgatcgactt gcggttcaag tcgatcgaat ccggccgcgg gtccatcagc
     1021 gacagcagtt cctgctcgtt atcgcaggag acgacctgca cgtctcggcc cggcttgatg
     1081 ccgtgctgct ccagatggcg ataggcgcga agcgtcacgc gatcgaccgg gatgaacacg
     1141 ccggttggtc gcggggatgt ggcgagccat ttctcgacga agcgctccga agccgcctcc
     1201 atcgcgggga tgctgttctg ctccgcgggg cgcaggggtt gaccgcagaa cgcgtgcacg
     1261 gtgcgctcac caccggacga ggcggcggcg gcttcgccgg cggcgcggaa acccatctcg
     1321 cgctcggcgt acatcgacag cgccgggtcc ggattgaaat acgcgatgtg ctgatgcccg
     1381 tggtcgatca ggtactgcgc ggccaatcgg ccgatctcct ggttgtccgg ccgcacgcgg
     1441 tcgccccacg cgtcaccggt gccggcgctg gcgcggctca tcatccacac ggcgggcacc
     1501 atcttgatct gcggcgtgat cgcatcggac gggaactcgc cataaccgag aatgccatcg
     1561 acattgccgg agcggatcgc ctgcggcaac tgcgcggccg actcgacgtg ggcgaagatc
     1621 agattcaaat tgcggtcggc cagcatccgc tgcacgtgcg ccagcacgga ggagctgatc
     1681 gacgagcctt cgcgcaggtg cagcagcgcg acgttgtgcg tgcgaatgcc atcggcggat
     1741 ttcggccggc gcccgcggcg gatttcttcc ggggcataac cgacgcgcgc gatggcggcc
     1801 ttgacggcgt gcaccgcctc gggtgaacgg cacggccgat tgttgatgat gcgccacgcg
     1861 gtggcgtagg gcaccttcgc ttcccgcgcg atctgcgaaa tcgacatggc cgattattat
     1921 gcgcgacgtg tagaaattaa gtaagagcgg ggctccttca aacgaaaagc ccgtggctct
     1981 ggcatgggac ggggtcttgc gaggtcggtc ccacggcata gccatgggct ttgttttgcg
     2041 gcgttgcgtg ggggcgcttc agcggacaca attgtgtccc tcagacacaa cagcaacagg
     2101 aagtgattga ctatgtccag gattcgcacg ccggatggta ttgagattgc ctcgttgaag
     2161 gggaagaaga cggaattctt cgacgcgatg gcgccggggc cgggcacacc gtgggaagac
     2221 cgcggcaccc gcggcgtggt cagcgcgttt ctcaagacct gcgcgatgag cctgacttcg
     2281 ttcttcaagc tggtcgatgc gattcgccgg ccggagacga ccaacgatgc attcgtgttc
     2341 ctcatcgact gctgcatcct gtggggcgtc agcgcgttta tccacgggct gatcttcatg
     2401 cacttcgccg ggaaggtgca gtaccacgag gtggagacgc agacgtacct gatgtacgtg
     2461 gcgatcgccg ccgtcggcgc gggcgggggg gtttacctgc tgttccggtt gtacaacatc
     2521 atctacgcga agctgatcgc gcaggagaag acggcgaccg cgttgccgga cgtgctgctt
     2581 tacaacgtga acgcgtacgc gctggggccg agcatcctcg cgccgatccc gttcatcggt
     2641 ccggcgctgg cgggtctgtt catcttcatc gacctgtaca tcgcggggcg aaagcggttg
     2701 aacatccgcg ccaccggcgc gttaatcgac gcgctgatgg catacgttgt ggtgctggtg
     2761 gtggccttcg tcgggtactg ggtcggcaac ctggtgttgc accaggtgct gggggatccg
     2821 attgacgacg tcacacccaa gacggcccaa acgcggtgag cacatctcgc gtggatcgcg
     2881 agcgccacgc gttgctgtgg cgcattcatc gccggtttcc gacagtgacc gcgacggttc
     2941 aattcgggcc ggtcacgttt ccgtttacgc gcgtcgccga tccggatcgc gtgctggacg
     3001 aggtggcgga ggaagaagac cgccgggagc ggctgggggg cgtgcgcctt gaggacccgc
     3061 tgcacctgcc gtattgggcg gagctgtggg acagcgccgc ggggatggcg caatatctcg
     3121 cgcgggaacc aactgcgatc gcgagcaagc gcgtgctcga cctgggctgc gggcagggct
     3181 tagctggttg cgccgcggcg gcgctggggg cgaacgtgct gttcgccgat ctcgaaccgc
     3241 cggcgctatt gttcgcgaag ttgaacagcc tgccgttctc accgcgagtc cgcacccggc
     3301 ggttgaactg gcagcgcgat cggctggacg agcgattcga cgtcatcctc ggggcggaca
     3361 tcctgtacga gcggaagcag tgggaattcc ttgagccgtt ctggcgagcg catttaagcc
     3421 atgccggttc agtgctccta ggcgagccgg gccggcagac gggtgattta ttcgtcgaat
     3481 ggattgccca gcgtggctgg tggttggagc gattcgaaga accggtgccc acgcgagacc
     3541 ggccgatccg catctttaag ctgaggttga gatgaaccaa atgcgcggcg acgcatgtaa
     3601 actttcccct tgcccggtac tccggagtac cgggcgaggg gaccgcggag cgtcgccttg
     3661 gtcggactcg cgtcgccggg cgccaagtca tgttgcactc atcgaaaagt gctacaaagt
     3721 gcaacacggt gcaacatttc gggtggcgga aaaaattgcg tgggccaaaa caagcggcca
     3781 aacgcaaaac gcccgcaacc tctttcgagg atggcgggcg ttaaagcacg gttgatccgc
     3841 tggtgccgtg tgagagccgt tcagtccaac cctaatgaaa aacggtgggt ggaagtcgat
     3901 cgaaacaaca gctctttcga gctgaggctt cgctgcggga agtcatcttc ccgcttcctg
     3961 caacgtttgc ttcccgtcaa cgtgtgccgg cgtcgatccg catacacaag accgtcgcaa
     4021 agcactcgtg cgggcatgcg cccggcaggc ggctaacccc tagggttgct tatcggttga
     4081 accaaatatg gctcccaatc tcgcacaccg cagaacatcc gtaccgcaga tactatcgac
     4141 cggattgggc gtcggcaatt acttaaatag tacggttgcg gggggtgggg gttcggggat
     4201 gaggggcgtg gggggggggg ggggggggtg gggggg
//
LOCUS       DATIMH010000212         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4088922, whole genome shotgun sequence.
ACCESSION   DATIMH010000212 DATIMH010000000
VERSION     DATIMH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4088922"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..919
                     /locus_tag="VLI90_03605"
     CDS             77..919
                     /locus_tag="VLI90_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSV13315.1"
                     /translation="MTESPAEYAARRLSGVIAERPRHEPPLALPVSLRSVQRSNISPG
                     KFDPPLANRILLVKWVIAGEAAMELHGQRFHFGPGDIAICTPGVPHAFWAVAPVNEMC
                     WFTVDGPLVEPFARHLDLSPGVYPYGAAPLQQIAELTDSLTDHSQHGRRRSSLLAIAL
                     LYDIGERVGNVELPAVVRQAQHIIEAEFANAELSTEGIAGRLNYHRGSLSRLFHQHTG
                     VTIIDYMTHVRLQEARLRLSESTDKVAEVGRRCGFRDPTYFCRWVRKHTGLSPKQLRL
                     QVSA"
     gene            1398..>2898
                     /locus_tag="VLI90_03610"
     CDS             1398..>2898
                     /locus_tag="VLI90_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSV13316.1"
                     /translation="MSTTELAKTYEPAAVEAEAYRVWMAEGAFVADPSRAGEPYSIVI
                     PPPNVTGALHLGHAINNTLQDILTRMHRMAGFNTVWIPGIDHAGIATQAVVEKQLKEK
                     ENQTRHDVGRDGLVARIWEWKQQYGDRILSQLQRLGCSCDWSRTRFTLDEQCARAVRE
                     TFFKLFKDGLIYRGKRLVNWDIHLQTSISDDEIYHETVKTSMWHIKYPIVSSTGVSPV
                     SGDARHGRDAHATEMVVATTRPETMLGDTAVAVHSEDPRWNWAIGKFVKLPLTDRLIP
                     IIADDILVDPKFGSGVVKVTPAHDPNDYAVYQRHLGQPDAIDIINILNSDGTINSNGG
                     VYAGMKRDAARKKVVADLEAQGLLAKEEPYETQVGHSDRSKTPVEPYLSDQWFVKMAP
                     LAEPALQVVRDGTIKFFPERHAQQYLSWLGEKRDWPISRQLWWGHRIPVWRRSQRLEQ
                     SLVQADIELVMTQLNRWRDQGKLAFQILAEGEQISPLEAADPNLALLYIC"
BASE COUNT          577 a          877 c          908 g          536 t
ORIGIN      
        1 gattccggaa ttgatttgca tctatttccc attccgttat cattgtttta tgtcactgac
       61 aacggtgagg gagcctgtga ccgaaagccc agcggaatat gcagccagaa ggctgtccgg
      121 cgtgattgcc gagcggccgc gacacgagcc acccctggcc ctgccggtgt cgctgcgcag
      181 cgtgcagcgg tcgaacatct cgcccggaaa gttcgatccg ccgctggcca accgcatcct
      241 gctggtcaaa tgggtgatcg ccggcgaggc cgccatggaa ctgcacgggc agcgcttcca
      301 cttcggtccc ggtgacatcg ccatctgcac gcccggcgtc ccgcacgcgt tctgggccgt
      361 cgcgccggtc aatgaaatgt gctggttcac cgtcgatggc ccgctggtcg agccgttcgc
      421 gcggcatctg gatctttcgc ccggtgtgta tccctacggc gccgccccgc tgcagcagat
      481 cgccgagttg accgacagcc ttaccgatca ttcccagcat ggccggcggc gctcgagcct
      541 gctcgccatc gcgctgcttt acgacatcgg cgagcgcgtc ggcaacgtcg agttgcccgc
      601 ggtcgttcgc caggcgcagc acatcatcga agccgaattc gccaacgcgg aactatcgac
      661 cgaaggcatc gccggccgat tgaactatca ccgcgggtcg ctcagccggc tgttccacca
      721 acacaccggc gtgacgatca tcgattacat gacgcacgtc cgcctgcagg aagcgcggtt
      781 gcgactgagc gaatcgaccg acaaggttgc cgaagtcggc cggcgctgcg ggtttcgcga
      841 tccgacgtac ttctgccggt gggtgcgcaa gcacacggga ctatcgccga agcaactgcg
      901 attgcaggtg tcggcctaat ctattgtgcc gcgccacgcc gggttcggag ggtactcccg
      961 acgaagcgag ctccttcgag cgcggcaaaa tccgggtcgt cggagtacct ccgagcccgg
     1021 cgtgggggtt gagccaagat ctgcgacgtc ttttgactgc cattgactgc cgcattgctc
     1081 gaaccgtcgc ggagcgataa acttccacgt cgaatgttca cactcctgcc cattccgatc
     1141 gtcgtacgat agggtcgccg gccctcgcct ggccgggctg acccccccat ctccgttaat
     1201 attccagcga atttcaacag tcgagaaata ccagcagatg attgaccacg gacaagacgg
     1261 aagaatgaac cacagatgca cacagataaa cacagataaa agcaattcag ttctcatctg
     1321 tgttcatccg tgttcatctg tggttccacc ctcttccgct ccgcgtctgc ggtgcctgaa
     1381 ttcgaaagag atttgaaatg agcaccaccg aacttgccaa gacctatgag cccgccgccg
     1441 tggaggccga agcgtatcgc gtgtggatgg ccgagggcgc gtttgtggcc gacccgtcgc
     1501 gcgcggggga gccgtacagc atcgtcatcc cgccgccgaa cgtgaccggt gcgttacacc
     1561 tgggtcacgc gatcaacaac acgctgcagg acatcctcac gcgcatgcac cgcatggcgg
     1621 ggttcaatac ggtttggatt cccggcatcg accacgccgg cattgccacg caggcggtgg
     1681 tcgagaagca gctgaaggag aaggagaacc agacccggca cgacgtgggc cgcgacggtt
     1741 tggtcgcgcg catctgggaa tggaagcagc agtatggcga tcggattctc tcgcaactgc
     1801 agcggctggg ctgttcatgc gactggagcc gcacgcgctt cacgctcgac gagcaatgcg
     1861 cgcgggccgt gcgcgagacg ttcttcaagc tgttcaagga tgggctgatt taccgcggca
     1921 aacggctggt gaattgggac attcatcttc aaacgagcat cagcgacgac gagatttatc
     1981 acgagacggt gaagacgtcg atgtggcata tcaagtatcc gattgtgagt agcacgggcg
     2041 tctcgcccgt gtctggcgat gctcggcatg ggcgagacgc ccatgctacg gaaatggtcg
     2101 tcgccaccac ccggcctgaa acaatgttgg gtgatacggc agtggccgtg cactcggaag
     2161 atccgcgctg gaactgggca atcgggaagt tcgtcaagct gccgctgacc gatcggctca
     2221 tcccgatcat cgcagacgat attcttgtcg atccgaaatt cggttccggc gtggtgaagg
     2281 tgacgccggc gcacgatcca aacgactacg cggtgtacca gcggcacctg ggtcagccgg
     2341 acgcgatcga catcatcaac atcttgaatt ccgacggcac gatcaattca aacggcggcg
     2401 tctacgccgg gatgaagcgc gacgccgcgc gcaagaaagt ggtcgccgat ctggaagcgc
     2461 agggactgct ggcgaaggaa gagccgtacg aaacgcaggt gggccacagc gatcgcagca
     2521 aaacgccggt ggagccgtat ttgtcggatc agtggttcgt gaagatggcc ccgctggcgg
     2581 agccggcgct gcaggtggtg cgcgatggga cgatcaagtt ttttccagag cggcacgcgc
     2641 agcagtattt gagctggctc ggggagaaac gcgattggcc gatctcgcga cagctgtggt
     2701 gggggcatcg gattccggtg tggcgccgct cgcagcgcct cgagcaatcg ctggtgcagg
     2761 cggacatcga gctggtgatg acgcagctca accgatggcg cgatcaggga aagctggcct
     2821 tccaaatcct ggccgagggc gagcagatct caccgctgga ggcggcggat ccgaacctcg
     2881 cgctgctgta catctgcc
//
LOCUS       DATIMH010000213         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_626559, whole genome shotgun sequence.
ACCESSION   DATIMH010000213 DATIMH010000000
VERSION     DATIMH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626559"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VLI90_03615"
     CDS             complement(<1..388)
                     /locus_tag="VLI90_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13317.1"
                     /translation="MFTPTADGGHARYSMELLRAMSDPPRGRWNFELVSSENLHDEFK
                     SDLYPVHPILPAIRDRSTFPNRFAWVVSRLMHYPRREAGFLKWLAEQDRLSGVHFQEW
                     TPWLAARVFRKIHRMGKRVFYTVHNIV"
     gene            complement(442..1983)
                     /locus_tag="VLI90_03620"
     CDS             complement(442..1983)
                     /locus_tag="VLI90_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HSV13318.1"
                     /translation="MQHREGKHVLSHAAIYLLARGLPGVIAFLTIPLFTRLLAPEEYG
                     RYALVLATVMLFNALLFQWLRLSMVRFLPACKDDPAQLKSALLTSSVLMLLALLPLAL
                     VAYFLPFARQWRPVIFPCWVMLALQSMFELCNEYSRAILRPWQYMGLQVARSGSILLV
                     GIAMVKFGAGWASPLIGMGVGMAAGVAYAYRRDWLDARLTIDRALLIKLCQYGIPLSL
                     TVALSMVISSSDRFLIAGFKGDAAAGLYSVAVDFTSQTLTLLMLVINLAMFPIAVRAW
                     ENHGRQAAQDAMKSNASLLMAVGMPCVVGLSAIAPGIAHCFLGKDYRQAAAGIIPLIA
                     LGTFVAGLKAYHFDAAFQFVHRTIYQVWIVLFAAIVNVGLNLVAIPRWGINGAAAASL
                     LAFIVSIALTAAVGRRHFALPFPLTSCLQVGLASAAMAAVLLPFRDRVGPLAVALQIT
                     GGAAVYGGILLAVNFLQLREPLLKRLGITGITEEAREHSDAGLAVGSTSSPSSISAAA
                     ALLEV"
BASE COUNT          429 a          674 c          674 g          319 t
ORIGIN      
        1 gcacgatgtt gtgcaccgtg tagaacacgc gcttgcccat tcgatggatt ttgcggaaga
       61 cgcgagccgc caaccacggc gtccactcct gaaagtgcac cccgctgagg cggtcctgct
      121 cggccagcca tttgaggaac cccgcctcgc gccgcgggta gtgcatcaac cgactgacga
      181 cccaggcgaa gcgattgggg aacgtgctgc gatcgcgaat ggccggtagg atcgggtgga
      241 cggggtagag gtcggacttg aactcatcgt gcaaattctc gctgctgacc agctcgaagt
      301 tccatcgccc gcgcggggga tcggacatcg cgcgcagcag ctccatgctg taacgcgcgt
      361 gaccgccgtc ggcggtcggg gtgaacatgc agatcgtttc tttttccgcg tgcactgtgg
      421 gtgcctccag acaaatgcgc gtcacacctc gagcagcgcc gcggcggcgc tgatggatga
      481 ggggcttgac gtggaaccga ccgccaatcc ggcatccgag tgctctcggg cttcttccgt
      541 gattccagta atgcccagtc gcttcagcag cggttcacgc agctgaagga agttcaccgc
      601 cagcaggatg ccgccgtaga ccgcggcgcc gccggtgatc tgcagcgcga cggccagcgg
      661 accgacgcga tcgcgaaatg gcaatagcac cgcagccatc gcggcgctgg ccaacccgac
      721 ctgcaggcaa gacgtgagcg ggaacggcaa tgcgaaatgc cgccggccca cggctgcggt
      781 cagcgcgatc gatacgatga acgccagcag cgacgctgcc gccgccccgt tgattcccca
      841 acgcgggatc gcgaccaggt tcaatcccac gttgacgatc gccgcgaaga gcacgatcca
      901 cacttggtaa atcgtgcgat gcacgaactg gaacgccgcg tcgaagtggt acgccttgag
      961 accagcgacg aacgtgccga gcgcgatcag cgggatgatc cccgcggcgg cctggcgata
     1021 gtctttgccg agaaaacagt gcgcgatgcc cggcgcaatc gcgctcagcc cgacgacgca
     1081 cggcatgccg acggccatca gcagcgatgc gttggacttc atcgcgtcct gcgcagcttg
     1141 ccggccatga ttctcccacg cgcgcacggc gatggggaac atcgccaggt tgatcaccag
     1201 catcagcagc gtgagcgttt ggctggtgaa atccacggcg accgagtaaa gccccgcggc
     1261 ggcgtcgcct ttgaagccgg cgatcaggaa acgatcgctg ctgctgatca ccatgctcag
     1321 cgccaccgtc agcgacagcg ggatgccgta ctggcacagc ttgatgagca gcgcgcgatc
     1381 gatcgtgagc cgggcatcga gccagtcacg acgatacgca tacgccaccc ccgcggccat
     1441 gccgacgccc atgccgatca gcggcgaagc ccaaccggcg ccgaacttca ccattgcgat
     1501 gccgaccagc aggatgctgc ctgagcgggc gacctggaga cccatgtatt gccagggccg
     1561 caggatcgcc cgcgaatatt cgttgcacag ctcgaacatc gactgcaggg cgagcatgac
     1621 ccagcagggg aaaatgaccg gccgccactg gcgcgcgaac ggcaggaagt atgcgaccag
     1681 cgccagcggc agcagcgcga gcagcatcag cacgctggag gtgagcagcg ccgatttgag
     1741 ttgggccgga tcgtccttgc acgcgggcag gaagcggacc atggacaacc gcagccactg
     1801 gaacagcagc gcgttgaaga gcatgacggt cgccagcacc agcgcgtaac ggccgtactc
     1861 ttcgggcgcc agcaggcgag tgaagagcgg gatcgtcaag aacgcgatga cgccgggcaa
     1921 cccgcgcgcg agcaaataga tcgcggcgtg tgataggacg tgcttacctt ccctgtgctg
     1981 cattgaaact cactctggtc ccctcgcccg gtactccggg agagggggta aggcctaata
     2041 cgctccatcg cggctcgcca gcgcgatgaa cgtctttcca acgatgtaca agtcca
//
LOCUS       DATIMH010000214         8550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4670122, whole genome shotgun sequence.
ACCESSION   DATIMH010000214 DATIMH010000000
VERSION     DATIMH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4670122"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1368)
                     /locus_tag="VLI90_03625"
     CDS             complement(13..1368)
                     /locus_tag="VLI90_03625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV13319.1"
                     /translation="MRIAFLNPIGDIGGAERSLLAWMAATRQAFAAAELTLVLPDDSG
                     ALAGAAREIGVKVKAVAMPAALARMGDSALIGSRAIAMLRLGGRALVALPALLLYIRR
                     LRRALREIQPDLIHSNGIKTHLLARLVLLRWRMPPGVTWHIHDFVGQRPLMARALRWA
                     ARPVTVAVAVSEAVAKDARLVLPRMRIDVVYNVTDVERFSPQPMGGERLDELAGLPTA
                     GNAVVRIVLVATYARWKGHDVFLRAAASARSHSTGVSPVPPLRFFIVGGPIYRTAGSQ
                     FSREELDELAEGLGLGSTPTTPGSEGSRDGPDPSRAPQNPGRRSTSEPGVLGFIAFQP
                     DPIDVYRAADIVVHASTRAEPFGLTIIEAMACGKPVIVSQAGGAAELFTDGEDALGVP
                     PGDAEQLAAAIVKLAQDADLRRRLGARGRQTVLRRFDQRLLPQAVSQFYRQLGAVPLA
                     P"
     gene            complement(1368..2543)
                     /locus_tag="VLI90_03630"
     CDS             complement(1368..2543)
                     /locus_tag="VLI90_03630"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSV13320.1"
                     /translation="MTKQSLYILCDLLEERWPSMDLAAEQLLAHLDAVARSDVQRLRP
                     VFRHRATKLAKASGAARNLDRLVNRLHDYPRMLRKSYGSSRDLFHLVDHSYSQLLHAL
                     PPGRVGVFCHDLDTFRSVLEPATERRPVWFRAMTRHILRGFQKAAVVFYSTNAVRGEI
                     ERFGLVDPVRLVQARMGVCEEFTAVKPAAEPGVSLLGEWAMREAFVLHVGSCIPRKRI
                     DVLLDVFAAARGRRRELRLVQAGGEWTAAQREQISRLGIESSVTQVRGLTREQLASLY
                     RSAAVVLQPSEAEGFGLPVAEALACGTAVLASDIPVLREVGGDMAAYAPVGDVAAWSE
                     ALLSMLDEPAALERPNERTAWASRYSWATHARIIGETYLRLSRGEPVAAPLTTAVAS"
     gene            complement(2548..3717)
                     /locus_tag="VLI90_03635"
     CDS             complement(2548..3717)
                     /locus_tag="VLI90_03635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HSV13321.1"
                     /translation="MAKQGMWDAIYPVPIPGAKFIPAAPQGSIAKPQYAAAAQSAGIH
                     GDPWRVVQAPPVCLLFAPLAMFPLHTAFLFYRFLSTICVWVVALYAGLIAGRIIGRPT
                     RLSGSVAALIGASPNSLATIRTGNVSSIVAALIAVAIYELQARDGWRGGAAALGAFVL
                     KYASFPLLPVAIVAGRLRTVAWAALLLLAFVLIPIPFIGVAPYREFAFKIAPHFDQLE
                     AYYGNQALRAVLARCWANADPPVTFLHAFSIVRWATFALFVFVLYSARCRIQTSARLI
                     ASASALMLCWFLIFSPVAHAHYYLYIFPLWGWLIIELRRVRFGLALATIAIALMWIPL
                     AGGRWSHLPLVISAHMLLAAIIMMVVALISIVRREADRGSMMSHSTAAIESTVSD"
     gene            complement(3876..4592)
                     /locus_tag="VLI90_03640"
     CDS             complement(3876..4592)
                     /locus_tag="VLI90_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSV13322.1"
                     /translation="MDTFGMVPPDASSLLDVGCNVGALLSDWHRRRPELRLAGVEVNA
                     EALASAKRDLSFADLQCAGAEALPFADGSFECVTCTEVVEHVPPELRAAAFREIHRVL
                     RPGGRLVLTTPHAGLFAFADSNNMRFRFGAVYRMVIRNGLRDRPYEGRGRAVEWHQHF
                     TLEELLQLAGDGWNVVAVQRGGLFLYPLMDWLSWPFYRMGRSDHWLRRLFQRIAGWDY
                     SVDFGRASYGIMIVLDKQTD"
     gene            complement(4596..6914)
                     /locus_tag="VLI90_03645"
     CDS             complement(4596..6914)
                     /locus_tag="VLI90_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV13323.1"
                     /translation="MGDRWVILTGEYPPQPGGVSDYTRLIAKGLAAAGDEVHVWAPPS
                     VGATPVDEGVRVHRLADHWLGRGRRDFERELLGIERPYRVLVQYVPQAFGCRGMNLGF
                     ALMLRRLARRGAAVWVMFHEVVFPLQRGAPLRHNVLGIVTRVMARIIARNADRLFVSI
                     EDWRRVLTPMTNGRPIDWLPVPSNVAQQRDDEAARRVRERIIAGRDLSAIIGHFGSCD
                     GPIEPLLRGTLQRVLSGDRRRIALLIGRGGDAFRERFIAENSTLADQVVATGDVPADQ
                     AAAHIAACDLLVQPYIDGISCRRSSAIAGLALGTPVVSCRGHLTEAFWASSGAMPLAE
                     TPDVARMAELVERFIGDQALRASVASKGSELYAERFHPQNTIDALTRRGRFSDHGPGA
                     HEPMVAEATTPLHGSVRPIVVMLVNGDDSSAMGIRARSLSAGVSNHFDIRLAYRSGRK
                     IQSMWRFFRLLRRTRPAVTYVLDMSFSGVLAACAHRLLSRRNRLIIDTGDAIYELAKS
                     MGRGRVGLMLTWALEHLGFAVADHIVVRGSYHRELLEAQGVRATFIPDGVDVQQFARG
                     DRARIRRELGIGDDITLVGIVGSCVWNDRLQMCYGWELIELLHLLKDQPVHGILVGDG
                     SGIAHLQQMCRERGVDGRLHLVGRVPYEQLPDWLAAMDICLSTQTNDVPGRVRTTGKL
                     PLYLAAGRYVLATRVGEAARVLPEEMLVEYEGVRDSSYPIRLERRIRTLLATPASDCR
                     ERCRAIAQETFDYQRLSERAAAMLGTVLKGSD"
     gene            complement(7010..8362)
                     /locus_tag="VLI90_03650"
     CDS             complement(7010..8362)
                     /locus_tag="VLI90_03650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV13324.1"
                     /translation="MKIAITTHAMQRIGGVETYLSDIIPALRQAGHELIIACERGAIS
                     PGGDAIDTRGLGVDVLAADAIGFDAVVDNVRRWRPDIVYAHGMLDPRLEWAIARLAPS
                     VFFIHNYHGTCISGMKRFAFPVMRPCTRTFGFKCFAHYLPRRCGGLSPITMWRQFWLQ
                     RARLAMVRRCDAVVTHSFHMYSEFARHGVDIDRLHRFVYYSAEQIGGAIERAPTFPLP
                     IAGDPSSSTSAMSNGSDDDAPLQLTFAGRLDRNKGVQMLIAAAPLAARQLRRRLHVNI
                     LGEGPARAQFERLAKRAAGDCPQVTFTFTGWLNREAVADHLRRTDLVVFPSVWPEPFG
                     LVGPEAGNLGIPVAAFAVGGVTGWLVDDVNGQLAPADPPTAAGLASAIARCLRDPQTY
                     MRLKRGAFEMATRFNRASHLSELSAVFDSVLHERGNRAGATASAEPSGTEAATVAVTR
                     "
     gene            complement(8359..>8550)
                     /locus_tag="VLI90_03655"
     CDS             complement(8359..>8550)
                     /locus_tag="VLI90_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13325.1"
                     /translation="LSSVVGLKRAIWLASRARKFVPQREATPFLVNVPAPRFDTTTNR
                     AATELPDERKQRQFGVAVP"
BASE COUNT         1701 a         2956 c         2588 g         1305 t
ORIGIN      
        1 cggcgacggt tgttatgggg cgagcgggac agcgcccagc tgccgataaa actgcgaaac
       61 cgcctgcggc aacagccgct ggtcgaagcg gcgaaggacg gtttggcgcc cgcgtgcgcc
      121 caaacgcctg cgcagatccg catcttgcgc cagcttcacg atcgccgccg ccaactgctc
      181 ggcatcaccc ggtgggacgc ccagtgcatc ttcgccatcg gtgaacagct ccgccgcgcc
      241 accggcttgt gagacgatca ccggtttgcc gcaggccatc gcttcgatga tggttaagcc
      301 gaagggttca gcgcgcgtgc tggcgtggac gacgatgtcc gccgcgcgat acacgtcgat
      361 tggatcgggc tgaaacgcaa tgaacccgag cacgccgggt tcggaggtac tccgacgtcc
      421 cggattctgc ggcgcgcgag aaggatccgg gccgtcgcga ctaccctccg aacccggcgt
      481 ggtcggggtc gaacctagcc ctagcccctc cgcgagttcg tccaactcct cgcgcgagaa
      541 ctgcgacccc gccgtccgat aaatcggccc gccgacgatg aagaagcgca acggcggcac
      601 aggcgagacg cccgtgctat gtgaacgcgc gctcgccgcc gcgcggagga acacatcatg
      661 ccccttccac cgcgcgtagg tcgcaacgag gacgatgcga accacggcgt tccccgccgt
      721 cggcagtccc gccagttcat cgaggcgctc gccgcccatc ggctgcggcg aaaaacgctc
      781 gacatccgtc acgttgtaca cgacgtcaat gcgcatccgc ggcagcacaa gccgcgcatc
      841 tttcgccacc gcctccgaca ccgcaaccgc caccgtcacc gggcgcgccg cccaacgcaa
      901 cgcgcgggcc atcaacggcc gctgtccaac gaagtcatga atatgccacg tcacccccgg
      961 cggcatgcgc cagcgcagca acaccagccg cgcgagcagg tgcgtcttga tgccgttgga
     1021 gtggatcagg tcaggctgaa tctcccgcag cgcccgccga agccgtcgga tgtaaagcag
     1081 caacgcgggt agggcgacga gcgcgcgacc cccgagccgc agcatcgcga tcgcgcgcga
     1141 gccaatcagc gcgctgtctc ccatccgcgc cagcgctgcc ggcatggcaa ccgctttcac
     1201 cttcacgcca atctcgcgcg ccgccccggc cagcgcgcca gaatcatccg gcaaaaccag
     1261 cgtcagctcc gccgccgcaa acgcctgccg cgtcgccgcc atccacgcca gcaggctgcg
     1321 ctcggctccg ccgatgtcgc cgatcggatt gagaaacgcg atgcgcatca ggatgccacc
     1381 gccgtcgtga gcggcgccgc caccggctcg ccgcgcgaga gtcgcagata ggtctcgcca
     1441 atgatccgcg cgtgcgtcgc ccaagaatat cgcgacgccc acgccgtccg ctcgtttggt
     1501 ctttcaagcg ccgccggctc atccagcatc gacaacagcg cctcgctcca cgccgcgacg
     1561 tcgcccaccg gcgcataagc cgccatatca ccacccactt cgcgcaacac cgggatatcg
     1621 ctcgccaaca ccgccgtccc gcacgccagc gcctcggcca ccggcaggcc gaatccttcc
     1681 gcctcgctag gctgcaacac gaccgccgcg gagcgataca gcgacgccaa ctgctcgcgc
     1741 gtcaacccgc gcacctgcgt cacgctcgac tcgatcccca gccgcgagat ctgttcccgc
     1801 tgcgccgccg tccactcgcc gcccgcctgc accagccgca actcccgccg ccgcccgcgc
     1861 gcggccgcaa agacatccag cagcacatcg atccgcttcc gcggaatgca ggaccccacg
     1921 tgcagcacaa acgcctcgcg cattgcccac tcccccagca acgaaacccc cggctccgcc
     1981 gccggcttca ccgcagtgaa ctcctcacaa acccccatcc gcgcctgcac cagccgcaca
     2041 ggatcgacga gcccaaaccg ctcgatctcc ccgcgcaccg cgttggtcga ataaaagaca
     2101 accgccgcct tctgaaaccc ccgcaggatg tgccgcgtca tcgcccggaa ccaaaccggc
     2161 cgccgctccg tcgccggctc aagcacgctg cgaaacgtat cgaggtcatg gcaaaacacc
     2221 ccgacgcgcc ccggagggag ggcgtgcagc agctgtgagt agctgtggtc caccagatga
     2281 aacagatcac ggctactacc gtaggacttc cgcagcatgc gtggataatc atgtagccga
     2341 ttcaccagac ggtcgagatt tcgtgcggcg ccgctggctt tagcaagttt cgtcgcgcgg
     2401 tgccgaaata ccggtcgaag gcgctgcaca tcagaccgcg cgacggcatc gagatgtgca
     2461 agcagctgtt cggcggccaa gtccatgctc ggccagcgct cctcaagcaa gtcgcacaga
     2521 atgtaaaggc tctgcttcgt cattctctca gtccgacacc gtggattcga tcgccgcagt
     2581 tgagtgggac atcatggacc ctcggtctgc ctcgcgccgg acaatcgaga tcaacgctac
     2641 gaccatcatg atgatggctg ccaaaagcat gtgagcgctg atcaccagcg ggagatgaga
     2701 ccatcgtcca ccggcgaggg ggatccacat caacgcaatc gcaatggtcg ccaatgcgag
     2761 accgaatcga acccggcgca attcgatgat caaccatccc cataatggga atatgtagag
     2821 gtagtagtgc gcgtgagcga caggactgaa gatcaggaac cagcagagca tcagcgccga
     2881 cgcgctggca atgagccgtg cggaagtttg aattcggcag cgggctgaat agaggacaaa
     2941 aacaaacagc gcgaacgttg cccaccgcac aatggaaaat gcgtggagga atgtcaccgg
     3001 cggatcggcg ttcgcccaac agcgggcgag cactgctcga agggcttgat tgccataata
     3061 cgcttcgagc tgatcgaaat gtggggcgat cttgaatgcg aactcgcgat agggggcgac
     3121 gccgataaat ggaatcggga tcaacacgaa ggccagaagt agcaatgccg cccatgcgac
     3181 agtgcggagg cgcccggcga cgatggcgac gggcagcagc gggaaggagg cgtattttag
     3241 aacgaatgcg cccagcgcgg cggcaccgcc gcgccatccg tctcgagctt gaagttcata
     3301 aatcgcaacg gcgatcagcg cggcgacgat ggatgataca tttccggttc gaatcgtggc
     3361 gagcgagttt ggcgaagcgc cgatcagagc cgcgaccgag ccactcaggc gcgtgggccg
     3421 tccaatgatg cgcccggcga tcaaccccgc gtacagcgcg acgacccaga cacatatagt
     3481 gcttagaaac ctgtaaaaga gaaaggcggt gtgcagcggg aacatcgcga gcggtgcgaa
     3541 cagcagacaa accggaggcg cctgcaccac gcgccaaggg tcgccgtgga tgcccgctga
     3601 ctgcgcggct gcggcatatt gcggcttcgc gatcgatcct tgaggagcgg ccgggatgaa
     3661 tttagcgcct ggaattggga ctggatagat cgcatcccac atcccttgct tcgccatcaa
     3721 accggccatg tagtactgcg tgaaatccgc ctctcccgcg ggggcgaaat gttcccgtac
     3781 gggaacgacg acacacaaaa ataccagtac aaccgcgtag caattgcggt tgaagccagc
     3841 ggtagaccgg tctggtgatg ccggcgaagt gtgcattagt cagtctgctt gtcgagcacg
     3901 atcatgatcc catacgacgc ccgtccgaaa tccaccgagt aatcccaccc cgcgatccgc
     3961 tggaataagc gtcgcagcca atggtcgctg cgacccattc ggtagaacgg ccagctgagc
     4021 cagtccatga gcgggtatag aaatagtccg ccgcgctgaa cggccacgac gttccatccg
     4081 tcgccggcca gctgcagcaa ttcctccagc gtgaagtgct ggtgccactc gaccgctcgt
     4141 ccgcgacctt cgtaaggccg gtcgcgcagt ccgttgcgga tgaccatccg ataaactgcg
     4201 ccgaagcgaa accgcatatt gttcgaatcg gcgaaggcga agaggcccgc gtgcggtgtc
     4261 gtgagaacca gccgcccgcc cggccgaagg acgcgatgta tttctcgaaa cgccgcggct
     4321 cgcagctcgg gcggcacgtg ttccacgact tcggtgcagg tgacacattc gaacgaacca
     4381 tccgcaaagg gcagtgcttc cgccccggcg cattgaagat cggcgaaaga gagatcgcgc
     4441 ttcgcgctcg ccaatgcttc cgcattcact tcgacgcccg cgagccgaag ctccggccgg
     4501 cggcgatgcc aatcagacaa aagcgcgccg acgttgcatc ccacgtccag cagcgacgac
     4561 gcatcgggcg gcaccatacc aaaggtatcc accggttaat ctgatccttt caacacggtg
     4621 ccaagcatag ccgcggcacg ctcgctcagc cgttgataat cgaatgtttc ctgtgcaatc
     4681 gctctacagc gctcacgaca gtcgctcgcc ggcgtggcga gtaatgttct gattcgtcgc
     4741 tcgagccgga tcggatagct tgaatcccgc acgccctcat actccaccag catctcctcc
     4801 ggcagcacgc gggcggcttc gccgacgcgc gtcgccagta catatcgacc cgcggccagg
     4861 tacagcggga gctttccggt cgtccgcacg cggccgggga cgtcgttggt ctgcgtcgac
     4921 aggcagatgt ccatcgccgc cagccagtcg ggcagctgtt cgtacggcac ccggccaacc
     4981 aggtgcaggc ggccgtcaac gccgcgctcg cggcacatct gctgcaggtg cgcgatgccg
     5041 gaaccatcgc ccacgagaat gccgtgcacc ggctggtcct tcagcaggtg cagcagctcg
     5101 atgagttccc agccatagca catctgcagg cgatcgttcc acacgcagga gccgacgatg
     5161 cccacgagcg tgatgtcgtc gccgatgccc agctctcggc ggatgcgcgc acgatcgccg
     5221 cgggcaaact gctggacatc caccccatcg gggatgaagg ttgcgcgcac gccttgagct
     5281 tcgagaagtt cccgatgata gctgccgcgg acgacgatgt ggtcggcgac ggcgaagcca
     5341 agatgttcca gcgcccacgt gagcatcagc ccaacgcgcc cgcggcccat ggatttcgcc
     5401 aactcgtaga tcgcatcgcc ggtatcgatg atgagccgat tacgccgcga cagcaggcga
     5461 tgcgcgcacg cggcgagcac gccggagaac gacatgtcca gaacgtaagt gacagcgggg
     5521 cgggtccgcc ggagcaggcg aaagaagcgc cacatggact ggatctttcg gccggagcga
     5581 taagcgaggc gaatgtcgaa atgattcgag acgccagcgg acaacgagcg agcgcggatg
     5641 cccatcgcgg aagagtcgtc gccgttcacg agcatcacaa cgatcgggcg cacagacccg
     5701 tgaagcggcg tggtcgcttc agcgaccatg ggttcgtgcg cgccaggccc atggtcgcta
     5761 aagcgaccac gccgcgtgag cgcgtcgatc gtgttctggg ggtggaagcg ctcggcgtac
     5821 aactcacttc cctttgatgc cacgctcgcg cgcagcgcct gatccccaat gaaccgctca
     5881 accagctccg ccatccgcgc cacatccggc gtttccgcca gcggcatcgc accgctgctt
     5941 gcccagaacg cttcggtcag atgaccgcgg caagagacga ccggagtccc cagcgccagc
     6001 cccgcgatgg cgctgctccg ccggcaactg atgccatcaa tgtacggctg caccagcaga
     6061 tcgcacgcgg cgatgtgcgc cgccgcctga tccgccggca catcgccggt ggcgacgact
     6121 tgatccgcga gggttgaatt ctccgcgatg aatcgctcgc gaaatgcatc acccccgcgg
     6181 ccgatcaaca gcgcgatgcg acgacgatcg ccgctcagca cgcgctgcag cgtgccgcgc
     6241 aaaagcggct cgatcggccc gtcacagctg ccgaagtgcc cgatgatcgc ggacaaatcg
     6301 cggccagcga tgatccgttc ccgcacgcgc cgcgccgcct catcatcgcg ctgctgcgca
     6361 acattactgg gcaccggcag ccaatcgatc ggccgcccgt tggtcatcgg cgtcagcacg
     6421 cgccgccagt cttcgatcga cacgaacaac cgatcggcgt ttcgcgcgat gatccgcgcc
     6481 atcacacgcg tgacgatgcc cagcacgttg tgccgcagcg gtgcgccgcg ctgcagcgga
     6541 aacaccacct cgtgaaacat cacccacacc gccgccccgc gccgggccag ccgccgcagc
     6601 atcagcgcga aacccaggtt catcccgcgg cagccaaacg cctgcggcac atattgcacc
     6661 agcacgcgat atggcctctc gatccccaac aactcccgct cgaagtccct tcgcccgcgg
     6721 ccaagccaat gatccgccaa ccgatgcacg cgcacgccct catccaccgg cgttgcccca
     6781 accgatggcg gggcccacac gtgcacctca tcacccgccg cggccagccc cttggcaatc
     6841 aagcgcgtat agtcactcac cccgcccggc tgcggcgggt attcaccggt gaggatgacc
     6901 catcgatcgc ccattcagac tcccaaagcg ccgccacata ttgcatctga cagcgcggcg
     6961 accgacctcg agcagttgat tgggattggt gtggccatcg catctctctc taccgagtga
     7021 ccgcgacggt ggcggcttcc gtgcccgacg gctcggctga tgcggtggcg ccggcgcggt
     7081 tgcctcgctc gtgcaagaca gaatcgaaca ccgcgctcaa ttccgacagg tgactggctc
     7141 ggttaaatcg cgtcgccatc tcaaaggcgc cgcgcttcag tctcatatag gtctgcggat
     7201 cgcgcaggca tcgcgcgatg gcggacgcca gtccggcggc cgtcggtggg tcagccggcg
     7261 ctaattgccc gttcacgtca tcgaccaacc agcccgtcac accaccgacg gcaaacgcgg
     7321 cgacgggaat gcccaggttc cccgcttccg gaccgaccaa accgaatggt tcgggccata
     7381 cgcttgggaa gacgaccagg tctgttcgcc gcaaatgatc cgccaccgct tcgcggttca
     7441 accagccggt gaacgtaaat gtgacctgcg ggcagtcgcc ggccgcgcgc ttggcgaggc
     7501 gttcgaactg cgctcgcgcc ggaccttcac ccaaaatgtt gacgtgcagc cgccggcgca
     7561 gttgccgggc cgccagaggg gcggcggcga tcagcatctg cacgcccttg ttgcgatcca
     7621 atcgccccgc gaacgtcagc tgcagcggcg cgtcatcatc gctgccgttg ctcatcgctg
     7681 atgttgatga tgatggatcg ccggcaatcg gcagcgggaa cgtgggggcg cgttcgatcg
     7741 cgccgccgat ctgctcggcc gagtagtaga cgaaccggtg caggcgatcg atgtcgacgc
     7801 cgtgtcgggc gaactcgctg tacatgtgga acgaatgcgt gacgaccgcg tcgcagcggc
     7861 ggaccatcgc cagccgggcg cgctgcaacc agaactgccg ccacatcgtg atggggctca
     7921 gcccaccgca gcgtcgcggc aggtaatgcg cgaagcactt gaagccgaac gtgcgcgtgc
     7981 acgggcgcat gacgggaaat gcaaatcgtt tcatcccgct gatgcaggtg ccgtggtagt
     8041 tgtggatgaa gaagacgctg ggcgcgagcc gcgcgattgc ccattccagc cgcgggtcga
     8101 gcatgccgtg ggcgtagacg atgtccggcc gccaccgccg cacattgtca acgacggcgt
     8161 cgaaaccgat cgcgtcggcc gccagcacat cgacgccgag cccgcgcgtg tcgatcgcat
     8221 cgccgcccgg cgagatcgcc ccgcgttcgc aggcgatgat tagctcatgc cccgcctggc
     8281 gcagcgcggg gatgatgtcg ctgaggtagg tttctacccc accgatccgc tgcatcgcgt
     8341 gggtggtgat ggcgattttc atggcaccgc caccccgaat tggcgttgct tgcgctcgtc
     8401 gggcaattcc gtggcagcgc gattagtggt cgtatcaaaa cgaggcgcag gcacgttgac
     8461 cagaaacggc gtcgcctcgc gctgcgggac gaacttgcgc gcacgtgacg ccagccagat
     8521 ggcgcgcttc agcccgacga ccgaggacag
//
LOCUS       DATIMH010000215         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_337419, whole genome shotgun sequence.
ACCESSION   DATIMH010000215 DATIMH010000000
VERSION     DATIMH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337419"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VLI90_03660"
     CDS             <1..1540
                     /locus_tag="VLI90_03660"
                     /inference="COORDINATES: protein motif:HMM:NF026034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HSV13326.1"
                     /translation="RQNIVNANSYMELNSSNLTTTTPVVAAPVFRIGTWDGTPLGFSN
                     ADKITIMHPTDVRMIPWAADSSGLTNFTVGVDPDSSFPMAEWHTQNGAAPFVDTDNRI
                     TFTLTSAQAATLQSLRIGVTRMQQGRPNINVNGSWSSSVQSQSSQPSSRGLTTGNWRG
                     NNSVYIFNIPSGTLHSGTNTIDISCASGSTGTLYSGYHIYDAIDLVNTSSLTNASVLK
                     TITLTPSNPTVLTNAQQQFTATAKDQFGAVIPANFAWSDINGVVDGTGLYTAPGATGS
                     DTVTATSGTVSGSTNVTIAPPLQVNSASFGYLTQQAVIITFNKDVDTTKLSNAFTLVN
                     LTTNTPVPAASIHLSYANKTGTFTFTTQLADGNYRATIPASATLTAGGEHLPSDLQLN
                     FFTLRGDANDDAKVDVGDLGPLSTNYGIVTSGATWAQGDFNYDGKVDVADLGALASNY
                     GKALATGSSDATAASTAAPASVFANTSILSATPKQMIAAPQASVAASIFSDTNQRLRG
                     VGLE"
     gene            1756..2496
                     /locus_tag="VLI90_03665"
     CDS             1756..2496
                     /locus_tag="VLI90_03665"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HSV13327.1"
                     /translation="MSFCAPMGSGHVVESLESRRMLWAGVPDVGFSQDGYVDSDLAYT
                     KFVEAVGGDKVVLIGTDAADNTVIERRTGSGALDPTFDGDGRKTLANYQPNYASLVQS
                     DGKVIVAVMTSPNADGFLARFNTDGSLDGTFGVSGIAPSMFGTNSTIALTQQPDGKLL
                     ALGSEVTQQAGALRIFLQHPGDQRATGGSGCVAKQQDPRGGQSAGFDRKLRLDRLSPE
                     RRRLDRYDARVQRLGVFQPGQRPLRRAG"
     gene            2493..>4464
                     /locus_tag="VLI90_03670"
     CDS             2493..>4464
                     /locus_tag="VLI90_03670"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV13328.1"
                     /translation="MTWRSFPNGSVAAVGYTTDASAATHYGVWKWASLGTAYDLFGDP
                     TFNGTGSVLDTGLPLTRVFAQPDGKVVAAGSLLSTSPYSVLVARFAANGAFDSSFGSL
                     ASGKWSGNFNGQGMLLSDVDFSTDGDYLAVLTSNTVQQTITEGAIKFGDDGPADTTAP
                     TASIGGPYTLPEGGTVTLDGTASSDVGGSGIGKYEWDFNYDGSTFQADANGAKPVFSA
                     VDRDGPNSATVALRVIDNAGNVSAPAVTTVTWLNVAPTAKFTGSTVTLGSAGTVTFSA
                     PSDPSPADVSAGFQYSYDFNNDGTFDVVDSSSASVAVPASYLTTSGAHAVKGRIKDKD
                     GGSTTYTATVQVNPPASGGSIAGVVFNDANANATKDSGEAGMANVKVYLDANKNGSFD
                     SGEAFTMTGSSGAYTFGNLAAGTYRVREVTPSGSRNTTPSAHYFDATLTAGQNVTGKN
                     FGNTTLVLIAGNVFNDLNASGGKDSGESALASIKIYFDANKNGAWDSTEKYVLSDSAG
                     NWSFNIPAGSYRVRQVLGSGYRISSPTTGYFDITASAGQSFTGRNFADTQKILISGNI
                     FNDANGNGSKDTGETGLGKWTVYADLNNNGKLDAGEANVLSDGSGNWAIKTLAAGTYV
                     IRIVRPTGWSQTKPANNAGQSVTLTSGQTKTGL"
BASE COUNT          895 a         1437 c         1426 g          706 t
ORIGIN      
        1 tcggcagaac atcgtcaatg ccaacagcta catggagctg aattcgtcga acctcacgac
       61 gacgacaccg gtggtggcgg cgccggtgtt ccgcatcggc acctgggacg gcacgccgct
      121 ggggttctcg aacgccgaca agatcacgat catgcacccg accgacgtgc gcatgatccc
      181 gtgggcggcg gattcgagcg gcttaacgaa cttcacggtc ggcgtggatc cggacagctc
      241 gttcccgatg gccgagtggc acacgcagaa cggcgctgcg ccgttcgtgg ataccgacaa
      301 ccgcatcacg ttcacgctga cgtccgccca ggcggccacg ctgcagtcgc tgcgcattgg
      361 cgtgacgcgc atgcagcagg gccggccgaa catcaacgtg aacggcagct ggtcgtcatc
      421 cgttcagtcg caaagcagcc agcccagttc gcgcgggctc accaccggca actggcgcgg
      481 gaacaactcg gtctacatct tcaacatccc cagcggcacg ctgcacagcg ggacgaacac
      541 gatcgacatc tcctgcgcca gcggatcgac cggcacgctc tactccggct atcacatcta
      601 cgacgcgatc gaccttgtga acaccagcag cctgaccaac gcgtccgtac tgaaaacgat
      661 cacgctgacg ccgagcaatc caacggtcct caccaacgcc cagcagcagt tcaccgcgac
      721 ggcgaaggac cagttcggcg cggtgatccc ggccaacttc gcctggtcgg atatcaacgg
      781 cgtcgtcgac ggcaccggct tgtacaccgc gccgggcgcc accggcagcg acaccgtgac
      841 cgccaccagc ggcacggtca gcgggagcac caacgtcacg atcgccccgc cgctgcaggt
      901 caacagcgcc agcttcggat acctgacgca acaggcggtg atcatcacgt tcaacaaaga
      961 tgtggacacg acgaagctga gcaacgcgtt cacgctggtg aacctgacga cgaacacgcc
     1021 ggtgccggcg gcgtcgatcc acctgagcta cgcgaacaag accggcacgt tcacgttcac
     1081 cactcagctg gccgacggca actatcgcgc gacgatcccc gccagcgcca ccctcaccgc
     1141 cggtggcgag catctgccca gcgacctgca gttgaacttc ttcacgcttc gcggcgacgc
     1201 gaacgacgac gcgaaggtgg acgtcggcga cctcggcccg ctcagcacca actacggcat
     1261 cgtcaccagc ggcgccacgt gggcccaagg cgatttcaac tacgacggca aagtagacgt
     1321 ggccgacctg ggcgcgctgg cgtccaacta cggcaaggcg ctggccaccg ggtcgagcga
     1381 cgccacggcc gcgtcaaccg cggcgccggc gtccgtgttc gccaatacgt ccatattgtc
     1441 ggcaacaccg aagcagatga tcgccgcgcc gcaagcgagc gtggccgcgt cgatcttctc
     1501 agatacgaac caacgcctgc gcggcgttgg attggagtaa gcacagcgac gcgcgtcaga
     1561 gctggccgag gctttcttca aaaaggcccg cggctctgcc gcgggacggg gcgcgacgaa
     1621 ttcgtcccac ggcagagccg tgggcttcgg accgtgcgcg gttgcccttc agcgcagccc
     1681 gcctctcgtt gtataatctg acagctggga atcttttttc atcaagtcat tgtttcgatc
     1741 gcgggaggaa ggcggatgtc gttctgcgca cccatgggtt ccggtcacgt cgtcgaatcg
     1801 cttgaatcgc gccgcatgct ctgggcgggc gtgccggatg tggggttcag ccaggacggg
     1861 tacgtcgatt ctgacctggc ttacacgaag ttcgtcgagg cggtcggtgg ggataaggtc
     1921 gtcttgatcg gcacggacgc ggccgacaac acggtgatcg agcggcgcac tggcagcggc
     1981 gcgctggatc caacctttga cggcgacggt cgcaaaacgc tggcgaatta tcagccgaat
     2041 tacgcgagcc tggtgcagag cgacggcaag gtcatcgtgg cggtgatgac gtcgccaaac
     2101 gccgacggat ttttggcgcg cttcaatacc gatggctcgc tggacggcac gttcggcgtt
     2161 agcggcatcg cgcccagcat gttcggcacg aactcgacga ttgcgctgac gcagcagcca
     2221 gacggcaaac tgctggcctt gggtagcgag gtgacgcagc aagccggcgc gctgcggata
     2281 tttctccagc acccagggga tcagcgcgcg acgggcggaa gtgggtgtgt tgccaaacaa
     2341 caagatcctc gtggtggcca atccgcaggg tttgatcgaa agctacggct cgatcgttta
     2401 tcgcctgaac gccgacggct cgaccgatac gacgctcgag ttcaacggct cggtgttttt
     2461 caacccggcc agcggccact acgccgagct ggatgacatg gcgatctttc cccaatggct
     2521 cggtcgccgc ggtgggatat acgaccgacg ccagtgccgc gactcattac ggtgtgtgga
     2581 agtgggcgtc gctcggcacc gcgtacgacc tgttcgggga tccgacgttc aacggcaccg
     2641 gctcggtgct cgacacgggc ctgccgctga cgcgcgtgtt cgcgcagccc gatggcaagg
     2701 tggtcgccgc gggttcgcta ttgtccacct caccctactc ggtgctcgtg gcgcgcttcg
     2761 ccgccaacgg cgcgttcgat tcatccttcg gctcgctcgc atcgggcaag tggtcgggca
     2821 acttcaacgg gcagggtatg ctgctcagcg atgtagactt ctccaccgac ggcgattacc
     2881 tcgccgtgct cacatccaac acggtgcagc agacgatcac ggagggcgcg atcaagttcg
     2941 gcgatgacgg tccagccgac acgacggcgc ccaccgcgtc aatcggcggt ccgtacacgt
     3001 tgcccgaagg tggcacggtg acgctggacg gcacggcatc cagcgacgtc ggtggaagcg
     3061 ggatcgggaa gtacgagtgg gattttaatt acgacggttc cacgtttcag gcggacgcga
     3121 atggcgcgaa gccggtgttt tcagctgtgg accgcgacgg gcccaactcg gcgaccgtcg
     3181 cgctgcgcgt gatcgacaac gccggcaacg tctcggcgcc ggccgtgacg accgtgacgt
     3241 ggctgaacgt cgcgccgacg gcgaagttta ccggctcgac ggtgacgctc ggctcggccg
     3301 gcacggtgac gttcagcgcc cccagcgacc cgtcgcccgc cgatgtctcg gccggcttcc
     3361 agtacagcta cgacttcaac aacgacggca cattcgatgt ggttgattcg tcgtccgcgt
     3421 cggtcgcggt gccggcgtct tatctgacga cgagcggcgc gcacgcggtc aaaggccgga
     3481 tcaaggacaa ggacggcggc tcgacgacgt acaccgcaac ggtgcaggtg aacccaccgg
     3541 cgagcggcgg ttccatcgcc ggcgtggtct tcaacgatgc caacgccaat gcgacgaaag
     3601 acagcggcga agccggcatg gcgaacgtga aggtttatct ggacgccaac aagaacggct
     3661 cgttcgacag cggtgaagcg ttcacgatga ccggcagcag cggcgcgtat accttcggca
     3721 acttggcggc tgggacgtat cgcgtgcgcg aggtgacgcc cagcggcagt cgcaacacga
     3781 cgccgagcgc gcactatttc gacgcgacgc tgacggcggg gcagaacgtc actggcaaga
     3841 attttggcaa cacgacgctg gtgctgatcg cgggcaacgt cttcaacgat ctcaacgcca
     3901 gcggcgggaa ggacagcggt gagagcgcac tcgctagcat caagatctac ttcgacgcga
     3961 acaaaaacgg cgcgtgggac agcactgaaa aatatgtgct gagcgatagc gccggcaact
     4021 ggtcgttcaa catccccgcg ggcagctacc gcgtgcggca ggtgctgggc agcggatacc
     4081 gcatcagctc ccccaccacc ggctacttcg atatcaccgc gtccgctggc caaagcttca
     4141 ccggccgaaa tttcgcggac acgcagaaga tcctgatcag cggcaacatc ttcaacgatg
     4201 ccaacggcaa cggcagcaag gacaccggcg aaaccgggct gggcaagtgg acggtctacg
     4261 cggatctgaa caacaacggc aagctggacg cgggcgaagc gaacgtgctg agcgacggca
     4321 gcggcaactg ggcgatcaaa acactcgccg ccgggacgta tgtcatccgg attgtccggc
     4381 cgaccgggtg gtcgcaaacg aaaccggcga acaacgccgg ccaatcagtg acgctgacga
     4441 gcgggcagac aaagacgggc ctga
//
LOCUS       DATIMH010000216         4731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4378622, whole genome shotgun sequence.
ACCESSION   DATIMH010000216 DATIMH010000000
VERSION     DATIMH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4378622"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..358)
                     /locus_tag="VLI90_03675"
     CDS             complement(44..358)
                     /locus_tag="VLI90_03675"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSV13329.1"
                     /translation="MSVPPNNATIQIKDKDRRRSERRPLVVEAWVSSPTAVDPLDREE
                     VTSVNVSRHGVAFDRRTPLPVGTFHIIDLSMGEQRMRCEARIMTCRKLDSGLFEIGAE
                     FC"
     gene            complement(431..2275)
                     /locus_tag="VLI90_03680"
     CDS             complement(431..2275)
                     /locus_tag="VLI90_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV13330.1"
                     /translation="MLIASVSLAAGTARGTSNFWNPHGNGDGTSWSDSKNWDSNTIPG
                     AASGTTNADTATFNISTPDTLVTIDLNRNIKSLTFDTAVASGFTIGSTSAPTLLLSSG
                     GIIQTTTTIANTQTVSAPLVIEGTNGTYTFTSAASDNSKLLDFNGAISGGAAGSTVLT
                     LNGSNTGNNTIAGVISNGSATNFGLTTGGSATWVLTRNNTYTGLLTLGGSGKLVAATT
                     NINGSSTTNGISFNGGTLQAASGGITTAKAVSMTGAGTFDTNGNASILSGNITGSGGA
                     LTKAGASSLTLSGAANAFSAGLTINNGSVIAANANSGVLGSGNVTFGGSNTPTLDLNN
                     NSPTVGLLVGTGSNGVVTNNNASLATSTLTLNGAGSQSFAGVIKDGSVAKVAMKMSGG
                     TQSLSGANSYTGGTTISGGTLLANSMDTTNGSLGTGAVIVNGGTLGGGSISSTGQVKA
                     AVTVNSGGKITAGDIGTIGKLATGAQTWNTGGTYVWDVSNATGAAGAGFDQLNFSSLS
                     IAAAGANSFTIKIVGDAIANFDHTKPFSFAIAVGGSNSISGFDATKFVLDTSGFTHNG
                     VVAGDFFAISTSNNNSELDINYVPEPSSLGLAAIGAASLLRRRRRSAR"
     gene            3196..3369
                     /locus_tag="VLI90_03685"
     CDS             3196..3369
                     /locus_tag="VLI90_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HSV13331.1"
                     /translation="MNTAKSLISRFIKGEEGVTLIEYGLLAALIAVACVTILGNLGTK
                     LNNSFNNVNSKIP"
     gene            3390..3560
                     /locus_tag="VLI90_03690"
     CDS             3390..3560
                     /locus_tag="VLI90_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HSV13332.1"
                     /translation="MRIRALIRRLIRDESGVTLIEYGLLAALLALACVTVLGSLGTKL
                     NNSFNNVNNAVP"
     gene            3557..4249
                     /locus_tag="VLI90_03695"
     CDS             3557..4249
                     /locus_tag="VLI90_03695"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HSV13333.1"
                     /translation="MIACFDASQSQRLLALEQGSSQPSVKELRCLNRGIDRRSENRVM
                     TPQWFWFIPLFIALGVAAATDLRSRRIPNALTLALASAGLLQAILLPARAGGIGLGGC
                     AAGLAVGLLVPFVFFAINALGAGDVKLLAAVGAWVGPHPILIIMLVAALIGGVLAIVQ
                     GLMQRRLALVVGNTALLAMNLLNVRRYGVAKVAAMNSEAPTLKHAMPYGLAIALATAL
                     FITARAAGWVHA"
     gene            4249..4704
                     /locus_tag="VLI90_03700"
     CDS             4249..4704
                     /locus_tag="VLI90_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HSV13334.1"
                     /translation="MSARRSIRPFDNRRDRRGGGVLIEAAFALIILVIMTGGMIEFGW
                     LIFVTHTATGAARSGARAAIVAGAVKSDVQTAVNNIMTAAGWATTKYTVQVDDKTGGT
                     TDVDPSSIAVGHDVAVHVKSTWANVGVSVFNFVSSTRAIDCVVVMRKES"
BASE COUNT          817 a         1358 c         1540 g         1016 t
ORIGIN      
        1 cagggcaggc tgctccgaac ccggcgtggc tttgcgtcgt tgctcagcag aactcagcgc
       61 cgatttcgaa aaggccgctg tccagcttcc ggcacgtcat gatccgtgct tcgcagcgca
      121 tgcgctgctc gcccatcgac aggtcgatga tgtgaaacgt gcccaccggc agcggggtgc
      181 ggcggtcgaa cgcgacgccg tggcgcgaca cgttcaccga ggtcacttct tcgcggtcca
      241 gcggatcgac ggcggtgggg gatgacaccc acgcctcgac caccaacggc cgccgctcac
      301 tgcggcggcg atccttgtcc ttgatctgaa ttgtggcgtt gttgggcgga acgctcatgc
      361 ttccgctatc ggcgttatgc gcgcccgatg tgagcgcgaa gaccgcgttg actgccggat
      421 aatccaaacg ttatcgggca ctgcgacggc ggcgacgcag caggctggcc gcgccgatgg
      481 cggccagacc gagcgacgac ggctcgggga cgtaattgat atccagctcg ctgttgttgt
      541 tagacgtgga gatcgcgaag aaatcgccgg ctacgacgcc gttgtgcgtg aagccgcttg
      601 tgtccagcac gaacttggtc gcgtcgaagc cgctgatcga gttcgacccg ccaacggcga
      661 tcgcgaagga gaacggcttg gtgtgatcga agttcgcaat cgcgtcacca acgatcttga
      721 tggtgaagct gttggccccc gcggcggcga tgctcagcga cgagaagttc agctggtcga
      781 accccgcgcc cgcggcgccc gtggcgttgg aaacatccca gacataagtg ccgccggtgt
      841 tccacgtctg cgcgccggtg gcgagcttgc cgatcgtgcc gatgtcgccc gcggtgatct
      901 tgccgccgct gttgacggtg acagcggctt tcacttgacc ggtggagcta atcgatccgc
      961 caccaagcgt tccaccgttg acgatgacgg cccccgtgcc gagcgagccg ttggtcgtgt
     1021 ccatgctgtt ggccagcagc gtgccgccgc tgatggtcgt gccgccggtg tagctgtttg
     1081 ccccactcag cgactgggtt ccaccgctca tcttcatcgc gactttcgca accgatccgt
     1141 ctttgatgac gccggcgaag ctttgactgc cggcgccatt gagcgtcagc gttgaggtgg
     1201 cgaggctggc gttgttgttc gtgacgacac cgttgctgcc ggtgccgacc agcagcccaa
     1261 ccgtggggct gttgttgttc aggtcaagcg tcggcgtgtt cgacccgccg aaggtcacgt
     1321 tgcccgatcc aagcacaccg ctgttggcgt tggcggcgat gaccgagccg ttgttgatcg
     1381 tgaggcccgc gctgaaggca ttcgcggcac cactgagcgt caggctgctt gcgccggcct
     1441 tcgtcagcgc accgccggag ccggtgatat tgccggagag gatggaagcg ttcccgttgg
     1501 tgtcaaaggt tcccgccccg gtcatgctca cggccttggc ggtggtgatc ccaccgctgg
     1561 cggcctgcag cgtgccgccg ttgaacgaga tgccgttggt tgtgctgctg ccattgatat
     1621 tcgttgtggc cgcgacaagc ttgccgcttc caccaagcgt aagaagcccg gtgtaggtat
     1681 tgtttctggt caacacccag gtagcggatc cacccgtggt caaaccaaaa ttcgttgcgc
     1741 tgccgttgga gataacgccg gcaatcgtgt tgtttccggt gttcgaacca ttgagggtaa
     1801 gaactgtgga acctgcggcg ccaccgctga tcgcgccgtt gaagtcgagc agcttgctgt
     1861 tgtcggacgc ggcggacgtg aatgtatagg tgccgtttgt gccttcaatg actaaaggcg
     1921 cgctgaccgt ttgtgtattg gcgatcgttg tcgtcgtttg aatgatgccg ccgctgctca
     1981 acagcagcgt tggagcgctg gtgctgccga tggtaaaccc gctggcgacc gcggtgtcaa
     2041 aggtgagcga tttgatattt cgattcaggt cgatcgtgac cagcgtgtcg ggcgtcgaga
     2101 tattgaacgt ggcggtatct gcattagttg tgccgctggc ggcaccgggg atggtatttg
     2161 aatcccagtt cttggaatcg ctccaactgg taccatcgcc attgccgtgg gggttccaga
     2221 aattactggt gccgcgcgcg gtgcccgcag ccaaggagac cgaggcgata agcaccgctg
     2281 ccttggcccg gtgggggcga ggcctgcgcg taaatagatt attccccaaa gtcacccaca
     2341 caacttctgc cctccgaggc acaaccacat tcgagaacgg aatcaaccat ccatggcggg
     2401 aacgaggcgg gtacccactg atcaagccac ccactggctt cgccgactcg tctggcggag
     2461 actactcgat cgaagagcaa aatccaacgg ttttacgagg attttttctc cagtttcgtg
     2521 cgtgcgcgtt aatctggtga agcaatagcg gcgcactgac gaatttaggt tgcgcaagtt
     2581 aacaaggcct aacgggtatc aacgatctag tgtgggtgaa cggacatctt tagctcgcgg
     2641 cccgcgggga tccaatctct atatgttcga cgggatgccg gtcagccgtc gccaaagatt
     2701 taaacgttct actccggcga ttgagcagct ctggcggcgc accgcgacaa cattgcccgc
     2761 agaaaaggat tgacattggc gatgctcgcc cgcaagatgc tgacggtttg cggggggaca
     2821 cgttttgtgg gtgccaggac agcgagtggg taactctctt tagtccgatt ggccacattt
     2881 cagcacggaa gctgattgaa ttcgatttga ccgcgccccc gttctgttga ttcgggagcg
     2941 cttcgggtca accgcgggtg cacgcgggct ggccttttgc gctgcgtttt tcgcggctca
     3001 aggtctgctg cgccgcgaca tggcttgggc tatgtgattg gatgatctcg ctagtgcgat
     3061 cattgcaaat gcattgatat ctgccacggg cgtggcaggt ctatcgtgaa tttctgccgg
     3121 cgcttcgacg ttcgaagcga tgccagtttg gcatgggctg ttttggggtt gggctacttt
     3181 caaaggagtg cggtcatgaa taccgccaag tctcttattt ctcgtttcat caagggtgaa
     3241 gaaggcgtca cgctgatcga gtacggcctg ctggccgcgc tgatcgccgt cgcttgcgtc
     3301 acgatcctcg gcaacctggg cacgaagttg aacaactcgt tcaacaacgt gaacagcaag
     3361 attccctgat ctcttatgag gtgaacgcta tgcgtatccg cgcattaatt cgtcgcttga
     3421 tcagggatga gtcgggcgtg acgttgattg aatacggatt gctcgcggcg ctgctggcgc
     3481 tggcgtgcgt gaccgtcctg ggttcgctcg ggacgaagct caacaactcg ttcaacaacg
     3541 ttaacaacgc cgttccttga tcgcctgctt tgatgcatcg caaagccagc ggctgctcgc
     3601 gctcgaacag ggttcgagcc agccttcggt gaaggagttg cgatgtctca atcgagggat
     3661 tgatcgtcga tcggagaacc gcgtaatgac gccacagtgg ttttggttca ttccgttgtt
     3721 cattgcgctc ggcgttgccg cggcgaccga tcttcgatcg cgccgcattc ccaatgcgct
     3781 cacgctggcg ctggccagcg cggggttgct ccaggcgata ttgctgccgg cacgagccgg
     3841 tggcattggt ctgggtggat gcgccgcggg attggcggtg gggctgctcg tgccgttcgt
     3901 gttcttcgcg atcaatgcgc tcggcgccgg cgatgtgaag ttgctggccg cagtgggcgc
     3961 gtgggttggg ccgcatccga tcctgatcat catgctggtc gccgccctca tcggtggtgt
     4021 gctggcgatc gtgcagggat tgatgcagcg ccgcctggcg ctggtcgtcg gcaacactgc
     4081 gctgctggcg atgaacctgc tgaacgtccg ccgatatggt gtcgcgaagg tggccgccat
     4141 gaacagcgaa gcgccgacgc tgaagcacgc catgccgtac ggcctggcca tcgcgctggc
     4201 cacggcgctg ttcatcaccg ctcgcgctgc cgggtgggtg cacgcgtgat gtccgcacgt
     4261 cgttcaattc gaccgttcga caaccgacgt gatcgtcgcg gtggcggcgt cctgatcgaa
     4321 gcggcgttcg cgctgatcat cctggtgatc atgaccggcg ggatgatcga gttcggctgg
     4381 ttgatcttcg tcacacacac cgcgaccggc gccgcccgca gcggcgcgcg ggcggccatc
     4441 gtcgcgggcg cggtgaagtc ggatgtgcag accgccgtca acaacatcat gaccgccgcc
     4501 gggtgggcca ccacgaaata cacggtgcag gtggacgaca agacgggcgg gacaacggac
     4561 gtcgatccgt cctcgatcgc ggtggggcat gacgtggccg ttcacgtgaa gtcaacctgg
     4621 gcgaatgtgg gcgtgagcgt gttcaacttc gtctcatcca cccgggcgat cgactgcgtc
     4681 gtcgtgatgc gcaaggaaag ttgagcgcgg gtgagggcgc gtggggcgcg t
//
LOCUS       DATIMH010000217         4180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2068859, whole genome shotgun sequence.
ACCESSION   DATIMH010000217 DATIMH010000000
VERSION     DATIMH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068859"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..>4180)
                     /locus_tag="VLI90_03705"
     CDS             complement(286..>4180)
                     /locus_tag="VLI90_03705"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSV13335.1"
                     /translation="TNCTFTGNTAQGGDDVDLAAISGGTGRGGAIEITGGGSLSLINA
                     TISGNQAVGGLSSFGLGRADGLGGGVSSDNAGFVISVENTIVAGNSATTGPDISATGG
                     VGSSGNNIIGSTSGIGGTPFSDGVNGDHVGVDPKLSPLASNGGATQTMATAANSPARD
                     AGSASGAPTADQRGFARSGAVDIGAFEYQNHAPSITSTAPSTAGTSQAFTYTITTSDA
                     DSDHLTITAPTLPAWMTFTDHGDGTATISGTPGLANAGANAVSVVASDGIATATQSFT
                     LTVADQAPVFTSTPITAASPGLAYVYQVMTSDADGDAVAIGAQTLPAWLTLVDHGDGT
                     ATLSGTPARGDIGANSVTVSANDGVAGATQTFTVTVANHAPIFTSTPITSMRAGQAYT
                     YNITTSDADGDLVAITAPTLPGWLSLVDHGDGTATLSGTPTLADLADNSVSLQADDGL
                     ATATQSCTLSATNHAPVFTSSAPTTANIGQTLTYTITATDADGDAVAITASTLPAWLT
                     LADIGDGTATLSGTPARGDLGANAVVLQVSDGIASSAQSFTLAVSNQAPAFTSTPVTA
                     AVVGQAYSYLVTTSDGNGDSLALSVAGSLPGWLTFIDNGDGTGTLNGTPSAGDVGAAA
                     MSFQASDGLATATQSFAINVSTVAAQPPVLTAVLPITGGAEDAPRTIDYATLLAASDA
                     TAVSGQPISFIVTSVVSGTLTKNGVDVTPGTTTLSAGESLVWTPAGNASGTLAAFTII
                     ASDGALTSAGPVTVSAQVQEVEQPPVAGDDAFSVLEDLSVTGNVLGNDSHIDGDSLTA
                     ALENGPSHGTLSLGGDGSFDYTPAPGFIGDDAFTYRASDGIAAGNVATVTIQVVQVLK
                     PGSFGFAAAAADVSTGQTITLTLSRSGGLDGAASVDVIVAGGSAVAGADFVLTAGTVQ
                     FADQQASATITLSVPASALSGADLTVRLRLQNATGGAAIDAAVAETVVTIHEIPEHAP
                     VVADVSVTRKPGAQVVLDVLAKATDADGDKLTVSIATQAGHGGVSVFDNGTPDNPYDD
                     KLVYVPTPGTCDDDSFQFNVADGKGGISTGTVRITVSGAGLDVDPNNVKVKALFVVGT
                     PGNDVIVFLRSRKGIGVVMNRRSQGLFKFSGRLIAYGRAGNDLIVDHNVERQCYFVGG
                     QGNDRLFGYNQADVMLGGAGNDTIGGGANNDILIGGAGSDFIVGATGDDLLVGGSTVY
                     DDDSPASRLALKDLSGTLGNRKRVVEQRWLSVTRSGGVGSGHARVAPETLLDDGSTDV
                     LGGGVGADWVVGRFSDGAKDRMLGKQAVDRSTDLG"
BASE COUNT          698 a         1476 c         1258 g          748 t
ORIGIN      
        1 gttcaacgaa attgcggaga cgaagggatt tggacagggg cgatcgcatg tggcgcgtca
       61 gtccgctccg tctgaatgca acgttttgca acgtaatgca acgttttcca atggattcat
      121 ttgattgttg ctatatctgc aattcgatcg tggtgcagga cttgtcgatg aacgtcttgc
      181 ttgaggcatc cgattccgtt gcagaaccgc gttgcatcga cctacgactc cgcgccttgg
      241 tgagcgttgc caccttgaag aagcagctca gcaacgtcaa ccacgtcacc ccaaatccgt
      301 ggagcgatcc accgcctgtt tgcccaacat gcggtccttc gccccatcgc tgaaccgccc
      361 aaccacccag tccgccccca ccccgccgcc caacacgtcc gtcgatccat catccagcag
      421 cgtctccggc gcgacgcgcg cgtgccccga cccaaccccg ccgctgcgcg tcaccgacag
      481 ccagcgctgt tcgacgacgc gcttgcggtt ccccagcgtc ccggacaaat ccttcagcgc
      541 caaccggctc gccgggctgt cgtcgtcata cacggttgat ccgccgacca gcagatcgtc
      601 gccagtcgcg ccgacaataa aatccgaccc agcgccgccg atcaggatgt cgttgttcgc
      661 cccgccaccg atcgtgtcat tgcccgcgcc gcccagcatg acgtcggcct ggttgtaacc
      721 gaagagccga tcgttcccct ggccgccgac gaaatagcat tggcgttcga cgttgtgatc
      781 cacaatcaaa tcatttcccg cccggccata cgcgatcaac cggccgctga acttaaacaa
      841 cccctgcgat cgccgattca tcacgacacc gatgcccttg cgactgcgca ggaaaacgat
      901 cacgtcattc cccggcgtgc caacgacgaa cagcgctttg accttcacat tgttcggatc
      961 gacatccaga cccgcgccac tgaccgtgat ccgcaccgtg ccggtggaaa tgccgccctt
     1021 gccgtcggcc acattgaatt ggaaactgtc atcatcgcac gtgcccggcg tcggcacata
     1081 aacgagcttg tcatcgtacg gattgtccgg cgtgccattg tcgaagacgg acacgccgcc
     1141 atgccccgcc tgagttgcga tgctcaccgt gagcttatcg ccatcggcat cggtcgcctt
     1201 cgccaggaca tcgagcacga cctgcgcgcc cggctttcgc gtcaccgata catccgcaac
     1261 aaccggcgcg tgctcgggaa tctcgtgaat cgtgacaacg gtctccgcca ccgcggcgtc
     1321 gatcgcggcg ccgccggtgg cgttctgcaa acgcaaccga accgtcaaat ccgcgcccga
     1381 cagggcgctg gcgggcacgc tcagcgtgat cgtcgccgac gcctgctggt cggcgaactg
     1441 caccgtgccg gcggttaaaa cgaagtcggc gcccgcgacg gccgatccac cggcgacgat
     1501 cacgtcaacc gatgccgccc catcgagccc gccgctgcgc gacaacgtca gggtgatcgt
     1561 ctgccccgtc gagacatcgg ctgccgcggc ggcgaagccg aagctgcccg gcttcagcac
     1621 ctgcacgacc tggatcgtga ccgtcgccac gttccccgcg gcgatgccgt cgctcgcgcg
     1681 ataagtgaac gcatcgtctc cgataaaccc aggtgccggc gtgtaatcaa acgatccatc
     1741 gccgccaagc gatagcgtcc cgtgcgacgg gccgttctcc agcgcggcgg tcaaactatc
     1801 cccatcgatg tgcgaatcat tgccgagcac gttgccggtg acgctcaaat cttccagaac
     1861 gctgaatgcg tcatcgcccg ccacgggtgg ctgctccact tcctgcacct gcgcgctaac
     1921 cgtcaccggc ccggccgacg tcagcgcgcc atctgatgcg atgatcgtaa aagccgccaa
     1981 tgttccgctg gcgttgccgg cgggcgtcca caccagcgat tcccccgccg atagcgtggt
     2041 ggttcccggc gtcacatcga cgccgttctt cgtcagcgtt ccgctgacga ccgaggtgac
     2101 gatgaagctg atcggctgcc cgctgaccgc cgtcgcatcg gatgccgcca gcagcgtcgc
     2161 gtagtcgatc gtgcgcggcg cgtcttcggc gccgccggtg atcggcaaca ccgccgtcag
     2221 caccggcggc tgagcggcga cggtcgatac gttgatggcg aacgactgcg ttgccgtcgc
     2281 caaaccgtcg ctggcctgaa atgacatcgc ggcggcgccc acatcgcccg cgctcggcgt
     2341 tccattgagc gtccccgtac cgtcgccgtt gtcgatgaac gtcaaccacc ccggcaaact
     2401 gccggcgacc gacagcgcga ggctatctcc attgccatcg ctcgtggtca cgagatacga
     2461 atatgcttgc cccaccaccg ccgccgtcac cggcgtgctg gtgaacgccg gcgcctggtt
     2521 cgacaccgcc agcgtgaacg actgcgcgct gctggcgatc ccgtcgctca cctgcaacac
     2581 caccgcgttc gcgcccagat caccgcgcgc gggcgtgccg ctgagcgtcg cggtgccgtc
     2641 gccgatgtcc gccagcgtca gccacgcggg cagtgtgctc gccgtgatcg ccaccgcgtc
     2701 gccgtcggca tctgtggcgg tgatggtgta cgtgagtgtc tgcccgatgt tggccgtggt
     2761 cggcgcgctg ctggtgaaga cgggggcatg attcgtcgcg ctgagcgtgc agctctgggt
     2821 cgccgtcgcc agcccatcat ccgcctgcag cgatacgctg ttgtccgcca ggtcggcaag
     2881 cgttggcgta ccgctcaacg tggccgtgcc atcgccgtga tcaaccaacg acaaccaacc
     2941 tggcaaagtc ggcgcggtga tcgccactaa atcgccgtcg gcatcgctcg tcgtgatgtt
     3001 gtacgtatac gcctgcccgg cccgcatgga cgtgatcggc gtgctggtga aaatcggcgc
     3061 atggttggcg accgtgaccg tgaacgtttg agtcgcgccc gccacgccat cgttggccga
     3121 cacggttacg gaattcgcgc caatatcgcc gcgcgccggt gtgccgctta acgtcgcggt
     3181 gccgtcaccg tgatcgacga gcgttaacca cgccggcaac gtctgcgcgc cgatcgcaac
     3241 cgcatcgccg tccgcgtcac tcgtcatcac ttgatacaca tacgccagcc ccggcgaagc
     3301 cgcggtgatc ggcgtgctgg tgaagacggg cgcctggtcc gccaccgtca gcgtgaacga
     3361 ctgcgtcgcc gtcgcgatgc cgtcgctggc gacgaccgaa acggcgttcg cccccgcgtt
     3421 ggcgagaccc ggcgtcccac tgatagtcgc ggtgccgtcg ccgtggtcgg taaacgtcat
     3481 ccacgccggc agcgtcggcg cggtgatcgt caggtgatcg ctgtcggcat cgctggtcgt
     3541 gatcgtgtac gtgaacgcct ggctcgtgcc ggcagtgctg ggggccgtgc ttgtgatcga
     3601 tggcgcgtgg ttctgatatt cgaacgcacc aatatcgaca gcgccgctgc gggcaaaccc
     3661 gcgctggtcg gctgtcggcg cgccgctggc gctgccggcg tcgcgcgccg gactgttggc
     3721 ggcagtcgcc atcgtctgcg tcgccccgcc gttgctcgcc agcggcgaca gtttcggatc
     3781 gacaccgacg tgatcgccgt tcacgccatc actgaatggc gtgccgccga tgccgctggt
     3841 gctgccgatg atattgttgc cagagctgcc gaccccgccg gtcgcgctga tatcggggcc
     3901 agtcgtcgcg ctattgccgg cgacgatcgt attttcgacc gagatcacaa agcccgcgtt
     3961 gtcgctcgag acgccgccgc ccaagccgtc ggcgcgacca aggccgaagg agcttaaccc
     4021 gccgaccgcc tggttgccgc tgatcgtcgc gttgatcagc gacagcgaac cgccgccggt
     4081 gatctcgatc gccccaccgc ggccggtgcc gccggagatc gcggccagat cgacgtcgtc
     4141 gccgccctgc gccgtattgc cggtgaacgt gcagttggtg
//
LOCUS       DATIMH010000218         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_914769, whole genome shotgun sequence.
ACCESSION   DATIMH010000218 DATIMH010000000
VERSION     DATIMH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914769"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VLI90_03710"
     CDS             <1..1434
                     /locus_tag="VLI90_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV13336.1"
                     /translation="IDGTGAINSVVVDSPGSGYTSTPSLTLSSAGQTTAGALGLNGVT
                     LTSLQLTNPGFDYNSPTISITGGSGTGATGNIAGSSTSWTLNANRGITLGVGGGGLYQ
                     TAGTTLTYGGVIGSTDPGPFTKSGAGTLTLTAPETYTGNTSVSAGTLNVASSSSLPSG
                     TNLTANGTAHLSNAAQTIGSLNGAGSVVIDGGTALSVNGGGTFSGAISSGGSVTVGGT
                     TALTLSGANTYTGATTVNAGATLNVSGSLAATNAVTSNGTTNFAGNTTAVTETQVLSS
                     LAIGNGATVSITPSTFPFTPTLLKPGTTTYGSGAKIDITNNEFATTGTASGALTQIQS
                     GQIFSSQTPDPNKAIGYIDLSGADAGKFEVRYTLKGDANLDGTVNVGDLGALATSYGL
                     AGSWANGDFTQDGVVNVGDLGALATNYGTQLASGPSAGGGGLAASPLAIVSSGAGGGS
                     AAVPEPSSLGLLAAAALLSQRRRRRRA"
     gene            complement(1864..>2699)
                     /locus_tag="VLI90_03715"
     CDS             complement(1864..>2699)
                     /locus_tag="VLI90_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSV13337.1"
                     /translation="STNSLQVSMMLVMALNPPIQRQIVLPMSKAVEIAYKSLRLRLSR
                     SLLVTSGIILALAFLMSILTTDAMTAGMRRWLQAYPQSTQYKQLAARRQELESASAQQ
                     SNKTATTELEQIRAQLIAPQRLREMMTSAGVPTTAAEIDANRIQTRWLIGLALLVAFV
                     GILNAMLMSVTERFREIGTMKCLGALDSFIIKLFLIESLFQGVVGTVLGVIVGALLAM
                     ASQTRAYGSFAWTNVPWMQILTAAGICLAVGIGLTVAGALYPAWQAARMQPIEAMRVE
                     T"
BASE COUNT          474 a          865 c          882 g          478 t
ORIGIN      
        1 attgacggaa ccggcgcgat caacagcgtg gttgtcgatt cgcccggcag tggttacacc
       61 tcgacgccca gcctgacgct cagctccgcc ggtcaaacga ctgccggcgc gctgggactc
      121 aacggtgtca cgctgacctc cctccagttg acgaatccgg gcttcgatta caacagcccg
      181 accatcagca tcaccggcgg ctcgggcacc ggcgctaccg gcaatatcgc cggctcgtcg
      241 accagctgga cgctcaatgc caaccgcggc atcacgcttg gcgtgggcgg tggcggactc
      301 tatcaaaccg ctggcactac gctcacctac ggtggtgtca tcggcagcac cgatccgggc
      361 ccgttcacca agagcggcgc cggcacgttg acgctgactg cgccggagac atataccggc
      421 aataccagcg tcagcgccgg cacgctcaac gtcgcgtcca gcagttcgct ccccagcggc
      481 acgaatttga cggctaacgg aaccgcccac ttgtcgaatg cagcgcagac gatcggcagc
      541 ctgaacggcg ccggttcggt tgtgatcgat ggtgggaccg ccttgagtgt caatggcggt
      601 gggacgttct ccggggcaat cagcagcggt ggctcggtga cggtcggtgg cacaacggca
      661 ctgacgctga gcggcgccaa cacctacacc ggcgcgacca ccgtgaacgc cggcgcaacg
      721 ctgaacgtgt cgggttcgct ggcggccacc aacgcggtga cgtccaacgg cacaacgaat
      781 ttcgccggca acaccaccgc ggtcaccgag acgcaggtgc tttcgtcgct ggcgatcggc
      841 aacggtgcga ccgtcagcat caccccttcg accttcccat tcacgccgac gcttctgaag
      901 ccgggcacca cgacctacgg cagcggcgcg aagatcgaca tcacgaacaa cgagttcgcc
      961 acgacgggca cggcctctgg agcgctgacg cagattcaga gcggacagat cttcagttcg
     1021 cagacgcccg atccgaacaa ggcgatcggt tacatcgact tgagcggcgc tgacgccggc
     1081 aagttcgagg tgcgatatac gctcaagggc gacgcgaacc ttgacggcac cgtgaacgtc
     1141 ggcgacctgg gcgcgctggc gaccagctat ggtttggccg gcagttgggc gaacggtgat
     1201 ttcacgcaag atggcgtcgt caacgtcggt gatcttggcg cgctggcgac gaactatgga
     1261 acgcagttgg cgtccggtcc gtcggccggt gggggtggtt tagccgcgtc gccgctggcc
     1321 atcgtctcaa gtggcgccgg cggtggctcc gccgccgtcc ctgaaccctc gtcgctcgga
     1381 ctactcgctg ccgcagccct cctgtcgcaa cgacgccggc gccgccgggc ctgatttgaa
     1441 tgcacgcgcg ccggtgcaaa caccggcgaa caacgagtcc ctccgctgcg gcggctcgcc
     1501 cctgaccagg cgggccgccg cccccttttg tggatgccac cgcccggtcg catctgttgc
     1561 ggtcgagacg gatgtgatct ttgccgcttg cggcttcgca gactgcgtgg cctggattgc
     1621 gaagccgcaa gcggcgaatc gtggggaaga tcatcatcgc cgccgcgttt tgcggcgctt
     1681 gccgctgagg cggcgggcat tggctttgca cgcgcgtctt gccggggcca tgccgggttt
     1741 catgcatttc gaaaatgatc gggcttcaaa aatcccattt gtagccgcga cgcctgcgtc
     1801 gccttgcttt acggatggga agaggcgacg cgggcgtcgc agcgacaggc aggtttggac
     1861 gctctacgtc tccacccgca tcgcttcgat cggttgcatg cgcgccgcct gccaggcggg
     1921 atagagcgcg ccggcgaccg tgaggccgat gccgacggcg agacagatcc cggcggcggt
     1981 gaggatttgc atccacggaa cattcgtcca ggcaaagctg ccgtaggcgc gggtttggct
     2041 ggccatcgcc agcagggcgc cgacgatcac gccaaggacg gtgccgacga cgccttggaa
     2101 gagactttcg atcaggaaca gcttgatgat gaagctgtcg agggcgccaa ggcatttcat
     2161 ggttccgatt tcgcggaagc gctcggtgac gctcatgagc atcgcgttga ggatgccgac
     2221 gaaggcgacc agcagcgcca ggccgatcag ccagcgcgtt tgaatacggt tggcgtcgat
     2281 ctccgccgcc gtggtgggca cgccggcgct ggtcatcatc tcgcgcaggc gctgcggggc
     2341 gatcagctgc gcgcggatct gttccaactc tgtggttgca gtcttgttgg attgttgagc
     2401 gctggcggac tccagctcct ggcgacgcgc ggccagctgt ttgtactgtg ttgactgcgg
     2461 atacgcctgc aaccaccgcc gcatgcccgc ggtcatggcg tcggtcgtga ggatcgacat
     2521 gagaaacgcc agcgccagga tgatgccgct cgtcaccagc aggcttcggc tgaggcgcag
     2581 tcgaaggctt ttgtaggcga tctcgaccgc cttgctcatc ggcagcacga tctggcgctg
     2641 gattggcggg ttcagtgcca tcaccaacat catcgacacc tgaagtgaat ttgtcgatg
//
LOCUS       DATIMH010000219         4237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1781852, whole genome shotgun sequence.
ACCESSION   DATIMH010000219 DATIMH010000000
VERSION     DATIMH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781852"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1213)
                     /gene="coxB"
                     /locus_tag="VLI90_03720"
     CDS             complement(149..1213)
                     /gene="coxB"
                     /locus_tag="VLI90_03720"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HSV13338.1"
                     /translation="MPRWFGTGETPVLLAGIIVCLLTTCLARADVPYYGTQSALNPAG
                     ATATNIDILWWRTFWLLTAIYVIVVILALIPMFRDWGLSGVPPEHISPTAREERRLIT
                     IVSSAVGITTMILFVLLFGDFRTGIKNRSLESEADPLTISVIGHQWWWDIAYQDPKPN
                     KWISDANEIHIPVNRPVRLDLSSADVIHSFWAPNLSGKKDLVPGHPTSLTIRAQRTGT
                     YWAQCAEYCGYQHAHMRLAVIVQTPEEYQAWAEAARQPAPTPVTELQKRGMQVFMGRT
                     CSMCHTIQGTRAGGRLGPGLTHIASRATIAAGSLPNTMGHLGGWIVDPQGIKPGTNMP
                     QNALSADDLQAVLEYLESLK"
     gene            complement(1255..1806)
                     /locus_tag="VLI90_03725"
     CDS             complement(1255..1806)
                     /locus_tag="VLI90_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSV13339.1"
                     /translation="MGHLIAISIIAVAVFVSCKREERDFRVQPPAAGTTYLPPATDFH
                     AGPTTEPTHVFNAYDENAYAMQQGKLYYENFNCVGCHFHGGGGIGPPLMDDKWIYGSE
                     PEQIFATIIQGRPNGMPSFRGKIPDDEVWRIVAYVRSLSGLANIQAAPGRSDEMQNAP
                     SPNSLQKGKPVNSSQPPSTEMPS"
     gene            complement(1901..2881)
                     /locus_tag="VLI90_03730"
     CDS             complement(1901..2881)
                     /locus_tag="VLI90_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HSV13340.1"
                     /translation="MCSPSRNPRVDIPYAGFGAYSRRPGFFSRRSIRVVGVPPNPART
                     GVLIVIVIGLIGRSVLASAATTQPLVLRIAADPNNMPFSNDREEGFENRIAKLIADDL
                     GARIEYTWWAQRRGFFRDAIKHGESDVVLGVPAENFQRVLPTRPYYRSTYVFVYRADR
                     GLDIRSFDDPALRKLKIGVPLVGGSSNSPPGQALADRGIIDNIVGFSLYNDYRTSNPA
                     AEMIHAVANGAIDVAVIWGPIGGYFAPRETAAPLKVVPVNAPPELSTPFAYDIAIGIK
                     RGHAELTGRINDILARRRSDIERILDEYGVPRLPIAAATEETSDAKPSSR"
     gene            complement(2862..>4237)
                     /locus_tag="VLI90_03735"
     CDS             complement(2862..>4237)
                     /locus_tag="VLI90_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSV13341.1"
                     /translation="FINTLDVQTCCVDAETGKELWKVKLGSINKGESITMAPLVVHGK
                     VYVGNSGGEFGVRGWLKCLDAKTGNVLWTAYSTGPDADCLIGPNFKPFYKMDQGTDLG
                     VTTWPPDHWKLGGGTVWGWVTYDPETNQIFYGTANPGSWNPELRPGDNKWSCTVFARD
                     ADTGQARWAYQYNPHDLFDHDEINEHIVLDLPLDGPDKPVRKVIVHPARNGYMYIIDR
                     VTGQVLSATTYERNTCIKSIDLETGRLIPNDEKHPQVGATIRDIQPASPGAKDWQPCA
                     FSPRTGLLYVPHQNLSCDYEAVEANYIEGTPYVGVNERMYAGADDPDALGYFTAWDLI
                     NHKLVWRIKETFPCWSGTVVTSGDVVFYGTMDGWFRALDARSGQKLWEFHCGSGIVGQ
                     PTTFKGPDGKQYVAILSGVGGWAGAVVAGDLDIRDPTGALGFVNAMKELPKYTTKGGT
                     LYVFALP"
BASE COUNT          707 a         1340 c         1374 g          816 t
ORIGIN      
        1 aggccgatct ccttgtggtc cgtcgatgtc atccatcccc agaagccggg cgcgcggctc
       61 caggtgatgt cgagcatctc ttccttcgac agcacgtccg ccggcgcggt cgggcgcggc
      121 tcggtggtga ttggttcggc agtggcggtc acttcaggct ctccagatat tccagcaccg
      181 cctgcagatc gtcggcggac aacgcgttct gcggcatgtt cgttcccggc ttgatcccct
      241 gcggatcgac gatccacccg ccgagatgac ccatcgtgtt gggcaacgac cccgcggcga
      301 tcgtcgcccg gctggcgatg tgcgtgaggc ccggccccag acgcccaccg gcgcgcgtgc
      361 cctggatcgt gtggcacatg ctgcaggtgc ggcccatgaa tacctgcatg ccgcgcttct
      421 gcagttccgt caccggggtc ggcgcgggtt gtcgcgcggc ctcggcccag gcttggtatt
      481 cctcgggcgt ctgcacgatc accgccaggc gcatgtgtgc gtgctgatag ccgcagtatt
      541 cggcgcactg tgcccaatat gtgccggtgc gctgggcgcg gatcgtcagc gacgtcgggt
      601 gccccggcac cagatctttc ttcccggaca ggttcggcgc ccagaagctg tggatcacgt
      661 cggcgctgga cagatcaagc cgcaccgggc gattcaccgg gatgtggatc tcgttggcgt
      721 cgctgatcca cttgttcggt ttcggatcct gataggcaat gtcccaccac cactggtggc
      781 cgatgacgga gatcgtcagc gggtccgcct cgctctcgag tgatcggttc ttgatccccg
      841 tgcgaaagtc gccgaacagc aggacgaaca ggatcatggt cgtgatgccg accgcgctgc
      901 tgacgatcgt gatcaggcgg cgttcttcac gggcggtggg cgagatgtgc tccggcggaa
      961 cgccgctgag gccccagtcg cggaacatcg ggatcagcgc gaggatgacg acgatgacgt
     1021 agatcgccgt aaggagccag aacgtgcgcc accagaggat gtcgatgttg gttgccgtcg
     1081 cccccgcggg gttgagggcg gattgcgtgc cgtagtacgg cacgtccgcg cgcgccaagc
     1141 atgtcgtcag aagacagacg atgatgccag ccagtagcac gggcgtctcg cccgtgccga
     1201 accaccttgg cacgggcgga acgcccgtgc gacttaattg tccgcctgat ctcctcatga
     1261 cggcatctcc gtcgaaggcg gttgcgacga gttcaccggc ttgcccttct gcagcgaatt
     1321 gggcgatggc gcgttctgca tctcatcgct gcgaccgggg gccgcctgga tgttggccag
     1381 cccgctcagg ctgcgcacgt acgccacgat ccgccagacc tcgtcatccg gaatcttccc
     1441 gcggaacgac ggcatgccgt tcggccggcc ctgtatgatt gtcgcgaata tctgctccgg
     1501 ctccgaaccg tagatccact tgtcatccat caacggcggc ccgatcccgc cgccgccatg
     1561 gaagtggcaa ccgacgcaat tgaagttctc gtagtagagc ttcccctgct gcatcgcgta
     1621 ggcgttctcg tcgtaggcgt taaagacgtg cgtcggctcg gtcgtcggcc cggcgtggaa
     1681 atcggtggcc ggcggcagat aagtcgtccc cgcggcgggg ggttggacgc ggaaatcgcg
     1741 ctcttcgcgc ttacaggaga cgaacacggc cactgcgatg atcgaaatag caatcaggtg
     1801 tcccatggca gagccatggg ctttatcatc gttgctacga tagcgcgacc gaatcccgcg
     1861 gctctgccgt aggacgggtt ggcgcgaagt tgggaaatgc tcatcgcgac gaaggtttcg
     1921 catcggacgt ctcctctgtc gcggccgcga tcgggaggcg cggcacgccg tactcatcga
     1981 gaatccgttc gatgtcactg cgtcgtcgcg ccagaatgtc gttgatccgg ccggttaact
     2041 ccgcgtgccc gcgtttgatc ccaatcgcaa tgtcataggc aaacggcgtg ctcaactccg
     2101 gcggcgcgtt caccggcacc actttgagcg gtgccgccgt ttcgcgcggc gcgaaatagc
     2161 caccgatggg accccatata actgcaacgt cgatggcgcc gttggcaacc gcgtggatca
     2221 tctccgccgc ggggttggac gtgcgatagt cgttgtaaag cgagaagccg acgatgttgt
     2281 cgatgatgcc gcggtccgcc agcgcctgcc ccggggggct gttgctgctg ccgcccacca
     2341 gcggcacgcc gatcttcagc ttgcgcaatg ccggatcgtc gaacgagcgg atatcgagcc
     2401 cgcggtcggc gcgatagacg aagacgtacg tcgagcgata gtacgggcgg gtcggcagca
     2461 cgcgctgaaa attctccgcg ggcacgccga ggacgacgtc gctttcgcca tgcttgatgg
     2521 cgtcgcggaa gaacccgcgg cgctgggccc accaggtgta ttcgatgcgg gcgcccagat
     2581 cgtcggcgat cagctttgcg atgcggtttt cgaagccttc ttcgcggtcg ttgctgaacg
     2641 gcatgttgtt ggggtccgcc gcgatgcgca gcacgagcgg ctgcgttgtc gcagcgctgg
     2701 caaggacact tcggccgatc aatccgatga cgatgacgat cagcacgccc gtccgcgccg
     2761 ggttcggagg tactccgacg acgcggatgc tgcggcgcga gaagaatccg ggtcgtcgcg
     2821 agtacgctcc gaacccggcg taggggatat cgacgcgcgg attacgggag ggcgaacaca
     2881 taaagcgtgc ctcccttggt ggtgtacttc ggaagctcct tcatcgcgtt gacgaaaccg
     2941 agcgcgccgg tgggatcgcg gatgtccaag tcgccggcga ccaccgcgcc ggcccagccg
     3001 ccgacgccgc tgaggatcgc gacgtactgc ttgccgtcgg ggcctttgaa tgttgtcggc
     3061 tgaccgacga tgccggaacc gcagtggaat tcccacagct tctgtccgct gcgggcgtcg
     3121 agcgcgcgga accagccgtc catcgtgccg tagaagacga cgtcgccgga cgtgacgacg
     3181 gtgccgctcc agcaggggaa ggtttccttg atccgccaga cgagcttgtg gttgatcaaa
     3241 tcccacgcgg tgaagtaacc gagcgcatcg ggatcatccg cgccggcgta catccgctcg
     3301 ttcacgccga cgtagggcgt gccttcgata taattcgcct cgaccgcttc atagtcgcac
     3361 gagaggtttt gatgcggcac gtacagcaag ccggtgcgcg gcgaaaacgc gcagggctgc
     3421 cagtccttcg cgcccggtga tgccggttga atgtcgcgaa tggtcgcgcc aacctgtggg
     3481 tgcttctcgt cgttcggaat cagccggccc gtctccagat cgatcgattt gatgcacgta
     3541 ttccgttcgt aggtcgtcgc cgacagcacc tgcccggtca cgcggtcgat gatgtacata
     3601 tacccgttgc gcgccgggtg cacgatgact ttccgcaccg gcttgtccgg cccgtcgagc
     3661 gggaggtcga gcacgatgtg ctcgttaatc tcgtcgtggt cgaacaagtc gtgcggattg
     3721 tattgatatg cccagcgcgc ctgcccggtg tcggcgtcgc gcgcgaagac ggtgcacgac
     3781 cacttgttgt cgcccgggcg cagctcggga ttccaactgc cggggttggc ggtgccgtag
     3841 aagatctggt tggtttcagg atcatacgtc acccagcccc acacggtgcc gccacccaac
     3901 ttccagtgat cgggcggcca cgtcgtcacg cccagatccg tgccctgatc catcttgtag
     3961 aacggcttga agttcggccc gattaagcag tcggcgtccg gcccggtgct gtacgcggtc
     4021 cacaggacgt tgccggtctt agcgtccagg cacttcaacc aaccgcgcac gccgaactca
     4081 ccaccgctgt tgccgacgta caccttgcca tgcacgacca gcggcgccat cgtgatggat
     4141 tcgcctttgt tgatcgaccc gagcttgacc ttccaaagct ccttgccggt ctcggcatcc
     4201 acgcagcagg tctgcacgtc cagcgtgttg atgaaga
//
LOCUS       DATIMH010000220         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3802169, whole genome shotgun sequence.
ACCESSION   DATIMH010000220 DATIMH010000000
VERSION     DATIMH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3802169"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..371
                     /locus_tag="VLI90_03740"
     CDS             69..371
                     /locus_tag="VLI90_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13342.1"
                     /translation="MIKSILILVLLLALAAGAFMTRPSQADFQNYMHQQNSGNGQLSL
                     KGLWNDYELENYLKSVTFHDRVLWVTVEKDGQRQYTGAFGHWFKSSSAAVPAAKPS"
     gene            complement(368..1762)
                     /locus_tag="VLI90_03745"
     CDS             complement(368..1762)
                     /locus_tag="VLI90_03745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HSV13343.1"
                     /translation="MPNVQFLPALRAAKPRRRLWLAGSALGLFLLTMVVGNAFVPRDK
                     AVTRAMLGHDFMAFYTAGAFVREGRYREMYNLDAVKAYEATLSRSAGLEVGSSFGPWW
                     NPPFYALVFEPLAALPYAKALDLWRWIGTGAFLIATTMLASFVARAASPCGFTRAGSP
                     CYLLVPLLILVSMPFLQAMSHGQNTFTSLLMLVIVVALWRQKRAWLGGLTAGLLFYKP
                     QLAAVVAAAIVFDLGWRALAGLCVTGVALLAVMMIAMPGAIGDYLHLLPANVRWMQVE
                     HMYLWERHVTLKAFWRLMLQGREAGEASVLTTGLTYLSVAAIGGSLAWVALRERFASA
                     RGRWPRLYMDDDVRRDRLITATIAVMPLLMPFYFDYDLLLLAVPATLYAVERIGRPEL
                     ARANDVWLTRAWVGLFVWLFVNPGLAMHTHVNVTVILLSCVAGLLIARSRRCDAQVVS
                     LSTSNKLTPFAAAA"
     gene            complement(2040..2969)
                     /locus_tag="VLI90_03750"
     CDS             complement(2040..2969)
                     /locus_tag="VLI90_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSV13344.1"
                     /translation="MTTATADLSVTQQPSPRKRRTRRFLLRVVKLSAIIYLVLCVLTY
                     CCQDWLMFPAHAYQGTPQAKIRLERDSEQVQLTTAHGDSVVAIFGRALLPDGSPHPDA
                     AHRPTLLYFYGNGGAIAWSMNEFDHFRRLGANVLVPDLVGFGMSSGKPSEANLYATAD
                     AAYDYLLQRRDIDPGKIVTVGWSMGGAIAIDLAARRPVAGVATFNAFTSMPEMAHLLL
                     PWLPAGTLCKYDFANERKMATIRVPAFICNGMRDTLVPPTMSDRLARAAQGPVTRVTI
                     DTADHNTIFVAKPLDLFAALQKFVDQVDAPIPR"
     gene            complement(3132..>3328)
                     /locus_tag="VLI90_03755"
     CDS             complement(3132..>3328)
                     /locus_tag="VLI90_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13345.1"
                     /translation="AEALVFYLFERTGASAPGYYAALMAVWSSAGVPRRRPDIAHLRV
                     ASSAAAWSTLLAVASSGGVE"
BASE COUNT          669 a         1104 c         1046 g          509 t
ORIGIN      
        1 gaaaaaacgt gtcccacggc atagcccggg ctttcgtggc gttggggggc tacgggtata
       61 attcgatcgt gatcaaaagc atcctcatcc tcgtcctgct gctcgccctg gcggctggcg
      121 ctttcatgac ccggccatcg caggcggatt tccagaacta catgcaccaa cagaacagcg
      181 gcaacggcca gctatcgctg aaggggctgt ggaacgacta cgagctggag aactacctca
      241 agtcggtcac gttccacgat cgtgtgttgt gggtcacggt cgagaaggac ggccagcggc
      301 aatacaccgg cgcgttcggc cactggttca agagcagcag cgccgcggtt cccgcggcga
      361 agccgtctta agcggcggca gcgaatggcg tgagtttgtt cgacgtggaa agcgagacga
      421 cctgcgcgtc acaccgccgc gagcgcgcta tgagcaggcc ggcaacgcac gacaacagga
      481 tgaccgtcac gttcacgtgc gtgtgcatcg ccaagcccgg gttgacgaag agccacacaa
      541 atagccccac ccaagcgcgg gtcagccaga catcattcgc gcgggccagt tccggccggc
      601 caatgcgctc gacggcatac agcgtcgccg gcactgccag cagcagcagg tcgtaatcga
      661 agtaaaacgg cataagcaac ggcatgacgg caatcgtcgc cgtgatcaat cggtcgcgcc
      721 gcacatcatc atccatgtaa agccgcggcc agcggccgcg ggcggacgcg aagcgttcgc
      781 ggagcgccac ccacgcgagc gatccaccaa tcgccgcgac gctcaagtaa gtcagccccg
      841 tcgtgagaac cgaagcctcc cccgcctcgc gcccctgcag cattaaccgc cagaacgcct
      901 tcagcgtcac atgccgttcc cacaggtaca tgtgctcgac ctgcatccac cgcacgttgg
      961 ccggcagcag gtgcaggtaa tcgccaatcg cgccgggcat cgcgatcatc atcacggcca
     1021 gcagcgccac gccggtcacg cacaatcccg ccagcgcccg ccagcccaga tcgaacacga
     1081 tcgccgccgc gacgacggcg gcgagctgcg gcttgtagaa gagtagccct gcagtcagtc
     1141 cccccagcca cgcgcgcttc tgccgccata gcgctacgac gatcaccagc attagcagcg
     1201 acgtgaacgt gttctgcccg tgactcatcg cctgcaagaa cggcatcgag acgagaatga
     1261 gcagcgggac caacaagtag cacgggctac cagcccgcgt gaagccgcac gggctggcag
     1321 cccgtgctac gaagctggca agcattgtag tagcaattaa aaaagcaccg gtgccgatcc
     1381 accgccacag atccagcgcc tttgcatacg gcagcgccgc cagcggctca aacaccagcg
     1441 cataaaacgg cgggttccac cacggcccga agctcgaacc gacttccaac cccgcggatc
     1501 gcgacagcgt cgcctcatac gccttcaccg cgtcgaggtt gtacatctcc cgatatcgcc
     1561 cctcgcgcac gaacgccccg gcggtgtaga aggccatgaa gtcatggccc agcatcgcgc
     1621 gcgtgacggc cttgtcgcgc gggacgaacg cgttgccaac aaccatggtc agcaggaaca
     1681 gccccagcgc gctgcccgcg agccacagcc gccgccgcgg ctttgccgcg cgaagcgcgg
     1741 gcaggaattg gacgtttggc attccgatgc cgttatatcg gccaaacgcg gcaagaagac
     1801 gcagggattg agggagagcg cgccggtgat agcccaggcc gggaggcctc ggatcggata
     1861 gcgagtggtg ccagttcgca gatcgatccc cggccttccg gcctgggcta ttaggattca
     1921 acgagccttc agatgtgctc tcgggaactc tccacgccgg gttcggagcg tacttgcgac
     1981 gacccggatg ctcgcgcggc agaatccggg tcgtcggagt acctccgaac ccggcgtggc
     2041 tagcgcggga tcggcgcatc cacctgatcc acaaacttct gcaacgccgc gaacagatcc
     2101 agcggcttgg cgacgaagat cgtgttgtga tcggcggtgt cgatcgtcac gcgcgtgacc
     2161 ggcccttggg cggctcgcgc caatcgatcc gacatcgtcg gcgggaccag cgtgtcgcgc
     2221 atgccgttgc agatgaacgc aggcacgcgg atcgtcgcca tcttgcgctc gttggcgaag
     2281 tcgtacttgc acagcgtgcc ggcgggcagc caggggagca gcaggtgggc catctccggc
     2341 atgctcgtga acgcgttgaa cgtggcgacg ccggcgacgg gccggcgggc cgcgagatcg
     2401 atcgcgatcg ccccacccat cgaccatccg accgttacga tttttcccgg gtcgatatca
     2461 cgccgctgca gcaggtagtc atacgcggcg tcggcggtgg cgtagaggtt cgcttccgac
     2521 ggctttccgc tgctcatgcc gaacccgacc aggtctggca ccagcacgtt ggcgccgagc
     2581 cggcggaagt ggtcaaactc gttcatcgac cacgcgatcg ccccgccgtt gccgtagaag
     2641 tagagcaacg tcggccgatg ggccgcgtcg ggatgtggcg atccatcggg caacagcgcc
     2701 cggccgaaga tcgcgacgac cgagtcgccg tgcgcggtcg tcagctgcac ctgctcgctg
     2761 tcgcgctcca gccggatctt cgcctgcggc gtgccctggt acgcgtgggc cgggaacatc
     2821 aaccagtcct ggcaacagta ggtcagcacg cacaggacca ggtagatgat ggccgagagt
     2881 ttgacgacgc ggaggaggaa ccggcgagtc cgccgcttcc gcggggaggg ttgctgcgtg
     2941 acagaaagat cggcggtcgc ggtggtcatt cgtccgacaa ggtctgcatt tcgtatgccg
     3001 cgtagcggta gccccgcagg ggcacgtgtt tttcgttcga gcgccaaaac atgtgcccct
     3061 gcgcggctac cgctacgcgg cacagagtca caccgcagaa cgtgacgcga aaatcgcggc
     3121 ggcgcgatca gttattctac gccaccgctt gacgctacgg cgagcagcgt tgaccatgcc
     3181 gccgcggatg aagcgacacg aagatgcgca atatctggac gccgtcgtgg aacccccgcc
     3241 gacgaccaga ccgccatcaa cgcggcgtaa tagccggggg cggaagcccc ggtccgttcg
     3301 aacaaataaa agaccagggc ttccgccc
//
LOCUS       DATIMH010000221         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_628611, whole genome shotgun sequence.
ACCESSION   DATIMH010000221 DATIMH010000000
VERSION     DATIMH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628611"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VLI90_03760"
     CDS             <1..1027
                     /locus_tag="VLI90_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13346.1"
                     /translation="VSSATNSFITQIGGFVGHLSTSDISGPDGVLVVNLPGQHSLYAG
                     DGNSTLKGFNVNAGYAPLPNTPLATGPASDKRVDEMAFNPTGQRLLVVNDAATPSPFI
                     SLINTTNNTIAVKTVFNGLNGTPNATMGLEQPVWDPTTQKFYLSMPQNNGTGPGAIAE
                     IDSTTGAVTHVFNLGDFGISSCGPTGLARGTGGRLLIGCGNAGTQSILFNPQANGGNG
                     AIIKTFSQVSGSDQVWYDPTTHRFFITAANNIGGPVLGIVGLDGTFLGDLTTSPGAHS
                     VAVDPVTGEIFVPFGGIAGNTTCPNGCIAVYTPNAAVPEPPTLPLLATFVTLLLGAAW
                     KTGRRTH"
     gene            complement(1136..1759)
                     /locus_tag="VLI90_03765"
     CDS             complement(1136..1759)
                     /locus_tag="VLI90_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13347.1"
                     /translation="MKLGLVAAWKKIAADHKQLSDKRFRPDLGPLLAKYETDFDEQDD
                     LHKQLDAIKSGFVPYLKAAAEARTETKNQLEDARKQLVDQFGADWQKFFNDYGDGSDW
                     EVVQKACNTYTTAQKKFIAAVDSYVKQLTQQRDTSAKGEQTAFTQYLTKRTQVEAKQK
                     KVLGELSKLQDEIPQVIGTYIKIAKQMKDDDLADDINSILRKLPVPA"
     gene            1943..2089
                     /locus_tag="VLI90_03770"
     CDS             1943..2089
                     /locus_tag="VLI90_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13348.1"
                     /translation="MQVIDPGIQDRSQRFWRHISIQLARALATQQDRRLPRGFDERER
                     VLTS"
     gene            2100..>2813
                     /locus_tag="VLI90_03775"
     CDS             2100..>2813
                     /locus_tag="VLI90_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13349.1"
                     /translation="MFRATKKVWLLALPLLAAWPAAGRASDNDTNTSRLFERPVSIQE
                     ISASSNPDPVDAIRCTYYPDFMIRESGTDSPAPNDAVFVPGVHPACSAAGPATGERVV
                     ATANYSLLGKIGRFVLFSATDPNGAVPFMVLDAATGRTIYTDATAADHGIDDVAADQR
                     TLHLQYMRGFNASCSIMQNPTGCWAKLVAEGKIPQAMTPPAPSPQLCVASYKQANATP
                     DDPSVVTYPVDMTIEASGRV"
BASE COUNT          511 a          920 c          807 g          575 t
ORIGIN      
        1 ggtctcgtca gctaccaaca gcttcatcac ccagatcggt ggcttcgtcg gccacttgtc
       61 aacctcggac atctccggcc ccgatggcgt gctggtggtg aaccttcccg gccagcactc
      121 cctctatgcc ggggatggca acagcacgct gaagggcttc aacgtcaacg ccggctatgc
      181 gcccttgccc aacaccccgc tcgccactgg ccccgcctcc gacaagcggg tggacgagat
      241 ggcgttcaat ccgacgggcc agcgcttgct ggtcgtgaac gatgccgcta ccccgtcgcc
      301 gttcatcagc ctgatcaaca ccaccaacaa caccatcgcc gtgaagaccg ttttcaacgg
      361 cctgaacggc acgcccaacg ccaccatggg gcttgagcag ccggtatggg atcccacgac
      421 ccagaaattc tatctctcga tgccgcagaa caacggcacc ggccccggcg ccattgccga
      481 gatcgactcc accaccggcg cggtgacgca tgtcttcaac ctgggcgact tcggcatctc
      541 gtcctgcgga cccacgggcc tggcccgtgg caccggaggc aggctgctga tcggttgcgg
      601 caatgccggc acgcaatcga tcctgttcaa cccccaggcg aacggcggga atggcgcgat
      661 tataaagaca ttctcgcaag taagcgggtc cgaccaggtg tggtatgatc cgacaacgca
      721 ccgcttcttc atcaccgcag cgaacaatat cggtgggcct gtactcggga tcgttggcct
      781 cgacggaaca ttcctgggtg acctcaccac ctcgcctggc gcgcattcag tggcggtgga
      841 tccggtaacc ggcgagatct tcgtcccctt cggcgggatc gccggcaata ccacctgtcc
      901 caacggctgc atcgccgttt acacccccaa cgcggcggtc cccgagccgc caacgctgcc
      961 gttgctggcg acgttcgtca cactgcttct cggcgccgcc tggaaaaccg gccggcgcac
     1021 ccactgaaga ccgactgctc gaaagtcgga gccgccggca tccccggcgg cttgacgtcc
     1081 caggacccgc cctggcgggg ctcactgccg gagcggcccg attatggcgc cctggctacg
     1141 ctggcacggg aagtttccgg aggatggaat tgatgtcgtc cgcgagatca tcgtctttca
     1201 tctgtttcgc tattttaata tatgtaccga ttacctgcgg tatttcgtcc tgtagcttcg
     1261 ataactcgcc gagtactttc ttttgcttcg cttccacctg agttcgcttc gtcaggtact
     1321 gagtgaacgc ggtttgttcg cctttggcgc tggtatctct ttgctgcgtg agctgcttta
     1381 cataggaatc aacagcggcg atgaatttct tctgcgctgt ggtgtaggta ttgcacgctt
     1441 tctgcacgac ttcccagtcc gagccgtcgc cgtagtcgtt gaagaatttc tgccaatcgg
     1501 ccccgaactg gtcgacgagc tgtttcctcg catcctcgag ctgatttttg gtttccgtcc
     1561 gcgcttccgc cgcagccttc aggtaaggaa cgaagccgga cttgatggcg tcgagctgct
     1621 tgtgaaggtc gtcctgctcg tcgaaatccg tctcgtactt ggcgagcagc ggccccagat
     1681 ccggccggaa ccgtttgtcg gacagttgct tgtgatcggc agcgatcttc ttccaggctg
     1741 cgaccaggcc gagtttcatg ttttcctccc acccgggttg cgccgcgggt taccactctc
     1801 atctgacaag aagcaagcag cccgccgctg tgaaccggtt cacatgctgc gtcgaggtga
     1861 cgacttcgtc ccgattgtct ggaagcggac atcggcaacc atcactgctt gcaatcgctc
     1921 gtcggttaca cgctgagatg cgatgcaggt gatcgacccg ggcattcagg acaggtcgca
     1981 gcggttctgg cggcatatca gcatccagct tgcgcgcgca cttgccacac agcaggatcg
     2041 ccgtcttccg cggggattcg acgaacgcga acgggttctg acatcgtgaa aggatgggtg
     2101 tgttccgggc aacgaaaaag gtttggctgc tcgcgctgcc gctgttggcg gcgtggcctg
     2161 cagcaggccg ggcatcggat aacgatacga atacgagcag gctcttcgag cgtccggttt
     2221 ccatccagga aatctccgcc agctcgaacc ccgatccggt ggatgcaatc cgctgcacct
     2281 actacccgga cttcatgatc cgtgagagcg gtaccgactc gcccgctccg aacgacgcgg
     2341 tgttcgttcc aggcgtccat ccggcctgca gcgccgctgg cccggcgacc ggagaacgcg
     2401 tcgtggccac ggcaaactac tcgttgcttg ggaaaatcgg ccgctttgtc ctgttttccg
     2461 cgaccgatcc caacggcgcg gtcccgttca tggtgctcga cgccgccacc ggccggacca
     2521 tctataccga tgcaacggcg gcggatcacg gcatcgacga cgtcgcggcc gatcaaagga
     2581 ccctgcatct tcaatacatg cgcgggttca atgcttcatg ctcgatcatg cagaacccca
     2641 cgggttgctg ggcgaagctg gtcgcggagg gcaaaattcc acaggcaatg acgccgccgg
     2701 ccccatcgcc gcaactctgc gtggcatctt acaaacaggc gaatgccacg cccgacgatc
     2761 ccagcgtcgt cacgtatccc gttgatatga ccatcgaagc gtccggcagg gtg
//
LOCUS       DATIMH010000222         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5536973, whole genome shotgun sequence.
ACCESSION   DATIMH010000222 DATIMH010000000
VERSION     DATIMH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5536973"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /gene="fabZ"
                     /locus_tag="VLI90_03780"
     CDS             <1..842
                     /gene="fabZ"
                     /locus_tag="VLI90_03780"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HSV13350.1"
                     /translation="IIYDFEAPAPIGRQTFGFTLGQDDFKGQLAPARTFVFEHEANEL
                     RARGMGKHLTPKELLVISPDGPIDNAFHWPNECVRHKVLDLIGDLYLVGRPLRGRIVA
                     YKSGHSLNHLLAKRLLEREQLTGRAAMVHRDAALDIRRIQRILPHRYPMLLVDRVLEI
                     QGDQKAIGIKNVTFNDIFFQGHYPGTPIMPGVLIVEALAQLGGILLSQKLEHTGKLAV
                     LLSMDKVKMRTPVTPGDQLLLEAVTVRVKSRTGHVRCKAFVGDKLAAEADIKFMLVDA
                     EPV"
     gene            912..1748
                     /gene="lpxA"
                     /locus_tag="VLI90_03785"
     CDS             912..1748
                     /gene="lpxA"
                     /locus_tag="VLI90_03785"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HSV13351.1"
                     /translation="MSKISPLAVVDPSARVADDVEIGPFCTVGPDVEIGPGNRLISHV
                     VITGHTTVGECNTFHPFAVIGGSPQDKKFRGEVSRLIVGSRNTVRESVTIHVGTALGG
                     GVTRVGDGNLLMVNSHIGHDSQVGSNCVIANNVMLAGHVNIGNYVVMSGGAACHHFVT
                     IGDYAFIAGMARIHHDVPPYVMISDNDRIRAINAEGLRRARFNDDEIDAIDEAARRLF
                     FNREKTFAAALAEFDTMNGLHPYVKTLVEFLRRRDIGKHGRYLEGLRLKKSYNSPPPP
                     MP"
BASE COUNT          402 a          611 c          613 g          382 t
ORIGIN      
        1 agatcatcta cgacttcgaa gcccccgcgc ccatcgggcg acagaccttc ggcttcaccc
       61 tgggccagga cgattttaag ggccagctcg cgccggcgcg cacgttcgtc ttcgagcatg
      121 aagcgaacga actccgcgcc cgcggcatgg gcaagcacct gacgccgaaa gagttgctcg
      181 tcatcagtcc cgacggcccg atcgacaacg cgttccactg gcccaacgag tgcgtgcgcc
      241 acaaggtgct cgatctcatc ggcgacttgt acctggtcgg ccggccgctg cgcggtcgta
      301 ttgtcgcata caagagcggg cattcgctca accatttgct ggccaagcgg ttactggagc
      361 gagagcagct gaccgggcgg gcggcgatgg tgcatcgaga tgccgctctg gatattcggc
      421 gaatccagcg tatcctgccc catcgctatc cgatgttgct ggtcgatcgc gtgttggaaa
      481 tccaaggcga tcaaaaagcc atcgggatta agaacgtcac gttcaacgac atcttcttcc
      541 agggccacta ccccggcacg cccattatgc cgggcgtgtt gatcgtggag gcgttggccc
      601 agcttggggg gatcctgctc agccagaagt tggaacacac aggaaaactt gcggtgttgc
      661 tgagcatgga taaggtgaag atgcgtaccc cggtgacgcc gggggatcag ctgctgctgg
      721 aagcggtgac ggtccgcgtg aaaagccgga ccggtcacgt ccgctgtaag gcgttcgtcg
      781 gggacaagtt ggccgccgag gcggacatca agttcatgct ggttgacgcc gagccggtgt
      841 gagaaatttc actttgagat ttcgaatttg aaatctccgc agaagaggga tcgaaaggtt
      901 tgtcgctgct gatgtccaaa atctcgccgc tggctgtggt ggacccgtcc gctcgcgtcg
      961 ccgatgacgt cgagatcggg ccgttctgca cggtcggccc cgatgtggag atcggcccgg
     1021 gcaatcggct catcagccac gtcgtcatca ccggtcacac caccgtcggt gaatgcaata
     1081 ccttccaccc ttttgcggtg atcggtggtt cgccacagga caagaagttc cgcggggaag
     1141 tgtcgcggtt gatcgtcggc agccgaaata cggtgcgcga atcggtcacg atccacgtcg
     1201 gcaccgccct tggtggtggc gtgacgcgcg tgggcgacgg caacctgctg atggtcaaca
     1261 gccacatcgg ccacgatagc caggtcggca gcaattgcgt gattgccaac aacgtgatgc
     1321 tcgcggggca cgtgaacatc ggcaattacg tggtgatgtc cggcggagct gcctgccatc
     1381 acttcgtgac gatcggggac tacgccttca tcgccggcat ggcccgcatc caccacgatg
     1441 tcccgccgta tgtgatgatt tccgacaacg atcgcatccg cgccatcaat gccgaaggac
     1501 ttcggcgggc acggttcaac gatgacgaga tcgacgcgat tgacgaagcc gcccgccggt
     1561 tgttcttcaa ccgcgaaaag acgttcgcgg cagcgctggc ggaattcgac acgatgaacg
     1621 gcctgcaccc gtacgtgaaa acgctggtcg aattcctccg ccgccgggac atcggcaagc
     1681 atggccgcta tctggaaggc ctgcgcttga agaagtccta caactccccc ccgccgccca
     1741 tgccgtaacc aatcgcccca cgtcgggttc ggaggtactc cgacggcccg gatttattct
     1801 ttcaagcttc aaagaaatgc gagcctgagt tgtagctgcg acgcctgcgt cgccttggtc
     1861 ttccgcgcga aagaggcgac gcaggcgtcg cagctacaac agatcccagc acatcaaagc
     1921 gcgtgattct tgaacacccc ttttagcggc ggcccgaagg gccgagcgtt cgcctggagc
     1981 ggacatccga caatcgccgc acgtcatt
//
LOCUS       DATIMH010000223         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4091141, whole genome shotgun sequence.
ACCESSION   DATIMH010000223 DATIMH010000000
VERSION     DATIMH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4091141"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VLI90_03790"
     CDS             complement(<1..1322)
                     /locus_tag="VLI90_03790"
                     /inference="COORDINATES: protein motif:HMM:NF020120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HSV13352.1"
                     /translation="MPMSPNQPPIHLRCEYFENPLVIDVPQPRLSWRLNDDRRGAAQS
                     AYEIHVASTPQLLADGKVDLWNSGKVASDLSIQVPYGGKPLQSRQRCHWAVRYWDAGD
                     APSPWSEPASWEMGLLNRDDWTANWIGAPIVGGPYTIPPAAYLRRAFSLNKRIAGARL
                     YVTALGLHEFEINGQRVGNDRLTPGRTEYLKRVPYHVYDVTALLAPGDNACGAVLGDG
                     WYCGHLHSDPRQTYGDRPRLLAQLEVQFDDRTSVTIASDSSWQSSEGPIRSSDLLMGE
                     DYDARQEMPGWSRPGFEASRWRPVELFDHPQIEIVAPRTPPVRPTRQVKPIAPPKVSA
                     NKRRWVFDLGQNIVGVARLKIRNAPPGHVIDLRYTEMLDKDGKPYTQALRTARAIDHY
                     TTKGGEEETYEPHFTFHGFRFVEVRDCPGTPTIDDLTGIVLHSDLPETG"
     gene            1967..>2762
                     /locus_tag="VLI90_03795"
     CDS             1967..>2762
                     /locus_tag="VLI90_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13353.1"
                     /translation="MSAAIVGLFFAHAGQARESWPVPDDAVKARLDTYDQHEKEVQEK
                     IKPELEAWAKKGKPYIPWASKPEDLPQAGIPAFPGAEGGGMYSFGGRGGKVYLVTSLD
                     DSGPGTLREACEAAGPRIVVFNVAGIIHLKMPIFIEAPYITIAGQTAPGDGICIADQA
                     LVDNAHDVVIRYLRLRRGNTDIYYRHGAHYGSPIGNIVLDHISASWGCDQNLDTYRHM
                     YQPPEGGPAKKLPAVNVTIQWCITSEALNTWNHAFGGDWGGRNTGFH"
BASE COUNT          520 a          861 c          893 g          488 t
ORIGIN      
        1 ccggtctcgg gaagatcgga gtgcaggacg atgccggtga gatcatcgat cgtcggcgtg
       61 ccggggcagt cgcgaacttc gacaaagcga aacccgtgaa acgtgaagtg cggctcgtag
      121 gtttcttcct cgcctccctt ggtcgtgtag tggtcgatcg cccgggcggt gcgcagcgcc
      181 tgcgtgtacg gcttgccgtc cttgtcgagc atctccgtat atcgcaggtc gatcacatgc
      241 cccggcgggg cgttgcggat cttcaggcgc gcgacgccga cgatgttctg gccgaggtcg
      301 aagacccagc ggcgtttgtt cgccgagact tttggcgggg cgatgggttt cacctgacgc
      361 gtcggccgaa ccggcggcgt gcgcggagcg acgatttcga tctgcgggtg atcgaacagc
      421 tcgaccggcc gccagcgcga cgcttcgaag ccgggccggc tccagccggg catttcctgg
      481 cgcgcatcgt agtcttcacc catcaacaga tccgacgaac ggatggggcc ttcgctcgac
      541 tgccaggatg aatcgctggc gatcgtgacg ctggtgcgat cgtcgaactg cacttccagt
      601 tgcgccagca gacgagggcg atcgccatac gtctgccgcg ggtcgctgtg caggtgaccg
      661 cagtaccagc cgtctccgag gaccgcgccg caggcgttgt cgcccggcgc aagaagcgcc
      721 gtcacatcgt acacgtgata cggcacgcgc ttgagatact cggttcgccc gggcgtcagg
      781 cgatcgttgc cgacgcgctg gccgttgatc tcgaattcat gcaagcccag cgcagtgacg
      841 tagagccgcg cgccggcgat gcgcttattg agcgaaaacg cccgccgcag atatgccgcg
      901 gggggaatcg tgtagggccc gccgacgatc ggcgcgccga tccaattcgc agtccagtca
      961 tcacggttga gcagccccat ctcccacgac gccggctcgc tccacggcga cggcgcatcg
     1021 ccggcgtccc aatagcgaac ggcccagtga cagcgctggc gcgattgcag cggcttgccg
     1081 ccgtaaggaa cttgaatcga cagatccgat gcgaccttgc cggaattcca aaggtccacc
     1141 ttcccatccg ccagcagttg cggcgtcgat gcaacgtgaa tctcataagc cgactgcgcc
     1201 gccccgcggc gatcgtcgtt caaccgccac gacagccgcg gctgcggcac atcgatgacc
     1261 agcggattct cgaaatattc gcagcgcaga tggatcggag gttggttcgg gctcatcggc
     1321 atttcagttt ccgcggccag cgttgccccg gccagcgggc gcggtgcgga aatgagtgta
     1381 cattctgtag gaactgttgc acttgatgag gaacaacagc gcgtcgagca acgcttcggc
     1441 gcggcgcgcg ttatggccta tggagtgtca cgccgccagc gccgcattct gctctggtcc
     1501 cctcgcccgg tacgccggga cagggtgagg gtgacttgga gctgtgaatg acctaatgtc
     1561 caaaagccaa atggcaatga agcgcatggc ccagcgggcg ctgcttggct ttggtcattg
     1621 ggaacttcat cgggatttgg agcttgagcc ttggtcatca accgctcgaa atcgccctca
     1681 ccgcagccct ctcccggagt accgggcgag ggggcaagac aaaacaccgc gctgccgcgc
     1741 aaagactact gggcgagatg cgtcgcgcgc gatcgcgcga gcgcctgcgc aacatcgcaa
     1801 taagtttacg caggttcgca agtcctcgtg tgtatgagat tgttagattg gccgcgcgcc
     1861 gcggtcgctg gtggaagcgg atcattgctc gcgcgcgttg tgctcatctc gaattgaaag
     1921 tcactggcga aacgaaacca tgttcagcaa aactgcaatt acagtcatgt cggcggcgat
     1981 cgttgggctg ttcttcgcgc acgcgggcca agcgcgcgaa agctggccgg tgccggacga
     2041 cgccgtcaaa gcccgcctcg acacctacga ccagcacgag aaggaagtcc aggagaagat
     2101 caagccggag ctggaggcgt gggcgaagaa gggtaagccc tacatcccct gggcttcgaa
     2161 gcccgaagac ctgccgcagg cgggaatccc ggcgttcccc ggcgccgagg gcggcgggat
     2221 gtacagcttt ggtggccgcg gcgggaaggt ctacctcgtc accagcctcg atgattccgg
     2281 tcccggcacg ctgcgtgaag cgtgtgaggc cgcggggccg cgcatcgtcg tcttcaacgt
     2341 cgccggcatc attcacctga agatgccgat cttcatcgaa gcgccgtaca tcacgatcgc
     2401 ggggcaaacc gcgccgggcg acggcatctg catcgccgat caggcgctgg tcgataacgc
     2461 gcatgatgtg gtgatccgct acctgcgact tcgccgcggg aacaccgaca tttactatcg
     2521 ccacggcgcg cactatggca gcccgatcgg caacatcgtg ctcgatcaca tctcggccag
     2581 ctgggggtgc gatcaaaatc tggacacgta tcgtcacatg taccagccgc cagaaggtgg
     2641 gccggccaag aagctgcccg cagtgaacgt caccattcaa tggtgcatca ccagcgaagc
     2701 gctcaacacg tggaaccacg cgttcggcgg cgactggggc gggcgaaaca cgggctttca
     2761 tc
//
LOCUS       DATIMH010000224         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5248298, whole genome shotgun sequence.
ACCESSION   DATIMH010000224 DATIMH010000000
VERSION     DATIMH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5248298"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1238)
                     /locus_tag="VLI90_03800"
     CDS             complement(75..1238)
                     /locus_tag="VLI90_03800"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HSV13354.1"
                     /translation="MSPKRVAILGCTGSIGCNTLDVIEHLGPPFRVVGLSAHTQTQKL
                     VEQVRRHRPAAVAVTDHDAAAKVGPALRDLGSTVHVGAEGMIELVQRDDVDIVVAAVV
                     GAAGLPAALAAVEAGKTLALANKESLVCAGSLLIPLARRKNVPILPVDSEHSAVFQAM
                     HCGRQREVRRVILTASGGPFRTATAERIRNATVEDAMNHPTWKMGGKITIDSATMFNK
                     GLELIEACWLFDLPPEQVEIVVHPESVVHSMVEFVDGSVIAQLSPPDMRTPIQYALTY
                     PERRDGNSRRLDISKAFDLHFEPPDFERFPALKIAYEVVRKGGTAGVVLNGANEAAVA
                     AFRSGKISFGEISRMVGLTIDRHDVQPEPSLDDLLRADRWARGCVETLIAGGD"
     gene            complement(1408..1947)
                     /locus_tag="VLI90_03805"
     CDS             complement(1408..1947)
                     /locus_tag="VLI90_03805"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSV13355.1"
                     /translation="MPSPQLFIFAGLPGVGKTTLARRLARELHAVYLRIDSIDQAMRD
                     EHLAIDGPAGYVVAYRLAEDNLRLGTPVVADSVNPIRITRRAWREVAVRAGVPFAEIE
                     VICSDAAEHRRRVESRPGDIAGLHLPTWDEVQTRERDAWETSPIIIDTAGQTEIDSFA
                     SLRRALIRCSGRGRPGCPG"
BASE COUNT          379 a          729 c          600 g          345 t
ORIGIN      
        1 gaagaagaag ctttatggcc atcttcgctt tcctgacccc taactcctga cccctaactc
       61 ctgacccctc actcctaatc gccaccggcg atcaaagttt caacgcatcc gcgggcccag
      121 cggtccgctc gcaggagatc gtccagcgac ggctccggct gaacgtcgtg ccgatcgatt
      181 gtaaggccca ccatccggga aatctcccca aacgagatct tcccggacct gaacgccgcc
      241 acggcggcct cattggcacc gttgaggacg acaccggctg tgccgccttt acggacaact
      301 tcatacgcaa tcttcaatgc cgggaaccgc tcgaaatccg gcggttcgaa atgcagatca
      361 aacgctttgc tgatatccaa ccgccggctg ttgccgtcgc gacgctcggg gtatgtcagc
      421 gcgtattgga tcggcgtccg catgtccggc ggcgacagtt gggcgatcac gctgccatcg
      481 acgaactcga ccatcgaatg caccaccgat tccgggtgca cgacgatctc cacctgctcg
      541 ggcggcaggt cgaacaacca gcacgcctcg atcagctcca gccccttgtt gaacatggtc
      601 gccgagtcga tcgtgatctt cccgcccatc ttccacgtcg gatggttcat cgcatcttcg
      661 acggtcgcgt tgcgaatgcg ctccgcggtt gccgtgcgaa acggcccacc gctggcggtc
      721 aggatcaccc gccgcacttc gcgttgccgg ccacagtgca tcgcctgaaa gaccgcagaa
      781 tgttccgaat cgaccggcag gatcggcacg ttcttccgcc gtgccagcgg aatcagcagc
      841 gaccccgcgc aaacgagcga ttccttattc gccaatgcca gcgtcttccc cgcctccacc
      901 gccgccagcg ccgccggcag ccccgccgcg cccaccacgg ccgcgacgac gatgtccaca
      961 tcgtcgcgct ggaccaactc gatcatcccc tccgcgccga catgaaccgt cgatcccaga
     1021 tcgcgcagcg ccggccccac cttcgccgcc gcgtcgtgat cggtgaccgc aacggccgcc
     1081 ggccgatgcc ggcggacctg ctcgaccagc ttctgcgttt gcgtatgagc cgacaacccg
     1141 accacgcgaa atggcgggcc caggtgctcg atgacatcga gcgtgttgca gccaatggaa
     1201 ccggtacaac cgaggatcgc gacgcgtttg ggggacaagg tgggcgtgat gatagcggcg
     1261 ggcgcgcggg ggacaagcgc ttgtcaggga gcgccgcatg ccccccacgc cgggctcgga
     1321 ggtattccga cgacccggat tcttctgcgc gcgagaacaa tccgggtcgt cggaatacct
     1381 ccgaacccgg cgtggggatg ccctccatca cccaggacat ccgggccgtc ctcggcccga
     1441 gcatctaatg agcgcgcgcc gaagcgatgc aaaactatca atttcagtct gacccgcggt
     1501 gtcgatgata attgggctcg tctcccacgc atcgcgctcg cgcgtttgca cctcgtccca
     1561 cgtgggcaga tgcagtcccg cgatatcgcc gggccgcgat tcgacgcgcc ggcggtgctc
     1621 ggcggcgtcc gaacaaatca cctcgatctc ggcaaacggc acgccggcgc gcactgcgac
     1681 ttcacgccac gcccgccggg tgatgcggat cgggttcacc gagtcggcga cgaccggcgt
     1741 gccgagccgc agattgtcct cggccaatcg ataagcgacg acgtatcccg ccggtccgtc
     1801 gatggcgaga tgctcgtcgc gcatcgcctg gtcgatgctg tcgatccgca ggtaaaccgc
     1861 gtgcagctcg cgagcaagcc gccgcgccag cgtcgtcttc cccaccccgg gcaggcccgc
     1921 gaaaatgaac agttgcggcg atggcatcag atgatggaga aaccttcagc atcacgaagc
     1981 gcgtcgatca tcgcaccaaa cggtacgtga tagccagagc ctgcgtcgtt tagccgccgc
     2041 ggcctcgcgg cgc
//
LOCUS       DATIMH010000225         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4671659, whole genome shotgun sequence.
ACCESSION   DATIMH010000225 DATIMH010000000
VERSION     DATIMH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4671659"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VLI90_03810"
     CDS             <1..222
                     /locus_tag="VLI90_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13356.1"
                     /translation="TLVLRETIYHLEGCTFDRCNWHIDWILLWGSPESIREIKALVTL
                     IEQAQQQQLPPDQLAQGAVQANPGGQTGR"
     gene            323..2023
                     /locus_tag="VLI90_03815"
     CDS             323..2023
                     /locus_tag="VLI90_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HSV13357.1"
                     /translation="MPRVNSRFLAVVFALLFSLPPFLASSALALQPDEIALIVNSNEP
                     RGRELANFYAQARHIPDNRILELNLTPSDDLPFKVYEEQVVPQVREFLRTGHLEQQVK
                     CFVTFYGVPIRIAARVSTPADSTELERIKITLNQTVEKIIPPVKKLEALAAQLDAQFA
                     PTPGDDLDQLSARADRAMKVIRGQMSTIADPRRQLEVGADLLTALEPLLGASAKVQHD
                     VIEARLHPATQPATQQAALQAMADQYTAAIERAKDLEQRRFDPKARDELRHVVAKNFG
                     LLQWIKLLRDQADYLDPKETTAAFDSELAMVRWTVYPRVRWCENPLYYAAKRQAINMP
                     TYMVTRLDAPTTDQVKRIISDSIKAETDGLKGKVVLDSRGLLIDKAQPTERGLAEYDE
                     SIRDLGHLIRKHTKLAVLADDNPDPLPANSADDVALYCGWYSLRHYIPECKFNPGAVG
                     YHIASFEMLTLHGPGEQGWVPGLINDGIAATLGPVAEPFVQLFPKPADFFPLLLTGKL
                     TLAEVYWRTTPAASWMLGLIGDPLYTPYKNNPQLQASDLPDRLRPMVSTTTQPIAAEK
                     "
     gene            2237..2410
                     /locus_tag="VLI90_03820"
     CDS             2237..2410
                     /locus_tag="VLI90_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13358.1"
                     /translation="MPVQLICPNLRCRKFLSVPDDVRGKLVKCQHCQTNFRVPDHRKA
                     EPAGAAAKPAAAK"
     gene            2536..2880
                     /locus_tag="VLI90_03825"
     CDS             2536..2880
                     /locus_tag="VLI90_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13359.1"
                     /translation="MSDAPLDLSVRPNRSTTLVRAGGALGIAGCMIGLLVFITACAGF
                     DAVFYFSPIPVLMGLAGLIMVIAGGVQQRTLKIEDMHVVAAFFVTLIATIGGLVEVAA
                     WRHWTIFFGQGM"
     gene            3020..3415
                     /locus_tag="VLI90_03830"
     CDS             3020..3415
                     /locus_tag="VLI90_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HSV13360.1"
                     /translation="MGFGDTTSAEVEAVATAIVDAAYKIHKRFGPGLLESAYRVCLAH
                     ELRTRGFTVQCEVAMPLEYEGVHLDCGFRIDLLVADLVIVEVKAVADFHPVHEAQLIT
                     YLKLTGIRLGILINFNKKFIKDGIERVAL"
BASE COUNT          661 a         1148 c         1038 g          648 t
ORIGIN      
        1 accctcgtcc tccgggagac gatctaccac ctcgaaggct gcaccttcga ccgctgcaac
       61 tggcacatcg actggatcct cctctggggc agccccgagt cgatccgcga gatcaaggcg
      121 ctggtcacgc tgatcgaaca ggcccagcag cagcagctgc cgccggacca gctggcccaa
      181 ggcgcggtgc aggccaaccc cggtggacag acggggcggt aacgcgcccg cgctcgcgcg
      241 atctatcgca actaacaagc ggcggcggat gattcagccg aatcatccgc cgccgttgtt
      301 ttgcatcgcc tacgcttcta gcatgccgcg tgtgaacagc agatttttag ccgtcgtctt
      361 cgcgctcctt ttctccctgc cgcctttttt ggcatcatcg gcgctggcgc tgcagcccga
      421 tgaaatcgcg ctgatcgtca acagcaacga accgcgcggg cgcgagcttg caaacttcta
      481 cgcgcaagcc cggcacatcc ccgacaaccg tattctcgag ctgaacctca cgccgtcgga
      541 tgacctgccg ttcaaggtgt acgaggagca agtcgtcccg caggtgcgcg agttcctgcg
      601 caccgggcat ctcgagcagc aggtcaaatg cttcgtcacg ttctacggcg tgccgatccg
      661 catcgctgct cgcgtctcga cgccggcgga ttcgacggag ttggaacgga tcaagatcac
      721 gctgaaccag acggtcgaga agatcattcc gccggtgaag aagctcgaag cgctggcggc
      781 ccagctggac gcacaattcg caccgacgcc gggggatgat ctggatcaat tgtccgcccg
      841 ggcggaccgg gcgatgaagg tcatccgcgg gcagatgagc accatcgccg acccgcgccg
      901 gcagctggag gtgggggcgg atctgctgac cgcgctggag ccgctgctcg gcgccagcgc
      961 gaaggtgcag cacgacgtga tcgaagctcg gcttcatccc gccacgcaac cagcgacgca
     1021 gcaggcggca ctgcaggcga tggccgacca atacaccgcc gcgatcgagc gcgccaaaga
     1081 cctcgagcag cggcggttcg atccgaaagc gcgagatgaa ctccgtcacg tcgtcgcgaa
     1141 aaacttcggc ctgctgcagt ggatcaagct gctgcgcgac caggcggact acctcgatcc
     1201 caaggaaacc accgcggcgt tcgacagtga attggcgatg gtgcgctgga cggtctaccc
     1261 gcgcgtgcgc tggtgcgaga acccgctgta ttacgcggcc aagcgccagg cgatcaatat
     1321 gccgacgtat atggtgacgc gattagatgc accgactacc gaccaggtga agcgcatcat
     1381 cagcgattcg atcaaggcgg agacggatgg gctgaagggg aaggtcgtgc tcgacagcag
     1441 agggttgctg atcgacaagg cgcagcccac cgagcgcggg ctggcggaat acgatgagtc
     1501 aatccgcgat ctcggtcacc tcatccgcaa acataccaag ctggcggtgc tggccgacga
     1561 caaccccgac ccgctgcccg ccaacagcgc cgacgacgtc gcgctctact gcggctggta
     1621 cagccttcgg cattacattc ccgagtgcaa gttcaatccc ggcgcggtgg gttatcacat
     1681 tgccagcttc gagatgctca cgctgcacgg ccccggcgag caggggtggg tgccgggcct
     1741 gatcaacgac ggcatcgccg ccacgctcgg cccggtggcc gagccgttcg tgcagctgtt
     1801 ccccaagccc gcggatttct tcccgctgct gctgaccggc aagctcacgc tggccgaggt
     1861 ctattggcga accacgcccg cggcgagctg gatgctgggc ctgatcggcg acccgctcta
     1921 cacgccatac aaaaacaacc cgcaactgca ggcgagcgat ttaccggacc gtctgcggcc
     1981 gatggtttcg acgaccacgc aacccatcgc cgctgaaaaa tagaacggcc ccgtttagcc
     2041 cggcgtgaca caacgccgag tttcgctgag aagtagaggc cgttcgcctt ctgaatcgaa
     2101 agggcctcga agacccacat aaacccggcg cgtgtgccca cgccggggtg aatggggttt
     2161 cctactgctg tgggggctgt ggaatcggct caaggcgtcg catcgccggg tcgataatga
     2221 cacgggagaa cccgctatgc ccgtgcagtt gatttgcccg aaccttcgct gccgcaagtt
     2281 cctcagcgtc cccgacgacg tccgcgggaa gctggtcaag tgccagcatt gccagaccaa
     2341 cttccgcgtg cccgaccatc gcaaggccga acccgccggc gccgccgcta agcccgccgc
     2401 cgcgaaataa cccttcctta actgtatagc cgcgaacggt gcttgcggtc tttctcttcc
     2461 gtcagtgttc gcgggtcgca ttgttcccgc agattttctt tgcccccgtc gcgcctcgcg
     2521 gtatcgttac cgctcatgag cgacgcacct ctggacctct ccgttcgccc caatcgttcc
     2581 accaccctcg tccgcgccgg cggcgcactc ggcatcgccg ggtgcatgat cggcctgctc
     2641 gtcttcatca ccgcctgcgc cggtttcgac gccgtcttct acttctcgcc catcccggtc
     2701 ctgatgggcc tcgccggcct gatcatggtc atcgccggcg gtgtgcagca gcgcacgctg
     2761 aagatcgaag acatgcacgt cgtcgccgcg ttcttcgtga cgctgatcgc aacgatcggc
     2821 ggcctcgtcg aagtcgccgc ctggcggcat tggacgatct tcttcgggca ggggatgtga
     2881 tctttggtgc aatcggaatt gaaccacgaa gaccacgaag agcacgaagc aatgacacag
     2941 cagcagaatt gactgtttcc tttttctcct cttcgtgtgc ttcatcccct tcgtggttca
     3001 atcaatcgga gaggtacacg tgggatttgg ggacacgacc agtgccgagg tggaggccgt
     3061 tgccacggca atcgtcgatg cggcgtataa gatccacaag cgcttcgggc cgggccttct
     3121 tgaatccgcc tatcgcgtct gcctcgccca tgaactgcga acgcgcgggt tcaccgttca
     3181 atgtgaagtc gccatgccgc tggagtacga aggcgtccac ctcgactgcg gcttccgcat
     3241 cgacctgctc gtcgccgatc ttgtcatcgt agaagtaaag gccgtcgcgg atttccatcc
     3301 cgtccacgaa gctcaactga tcacctatct caagctcacc ggcatacgcc tgggcatcct
     3361 gattaacttc aacaagaagt tcatcaagga cgggatcgag cgggtggcgt tgtaacgcga
     3421 gcgttgcttg gtgcccttcg tgccttgatt cacttggtgc gtacctccgg gtctccattc
     3481 atggatcatc gaatc
//
LOCUS       DATIMH010000226         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4091364, whole genome shotgun sequence.
ACCESSION   DATIMH010000226 DATIMH010000000
VERSION     DATIMH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4091364"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VLI90_03835"
     CDS             <1..1457
                     /locus_tag="VLI90_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV13361.1"
                     /translation="GWLRVLGQRAGASDVDFGVSLVILGDVLFFMMLVWSTAGLLRRA
                     DVHRQQTQTALAGERTLLRGLIDNLPDHIFIKDAEGRYITDNISHAQFVGLSSPEQVV
                     GKTVADFFPPEVAAKFAADDAAVVNSARPLVSRVEVITEHDGSRRWVATTKVPVRDAA
                     GGVTGLVCISRDITDQVRADELLREQNVKLQDLARSERDAHETLKRAQSQMVQSEKLA
                     GLGQLVAGVAHEINNPLSFVNNNVAVLQRDVGAIAKLLKMYRQADPLVQAGNAELMSE
                     IRDLCDAIDIDYTLTSIDELTMRSREGLKRIQQIVKDLRDFARLDESDLHEVDLNHGV
                     ESTVNIIRGRAKSKQVLLELDLHPLPPVACFPAKVNQVIMNLLANAIDACRDGGKVTI
                     STHALDNGDGVRVEVADTGSGIPPDVRERIFDPFFTTKKQGEGTGLGLSISYGIIQDH
                     GGTIEVESKEGEGSRFTVTLPLHASIPSRRNKSG"
BASE COUNT          293 a          485 c          494 g          248 t
ORIGIN      
        1 tcggctggct ccgcgtgctc ggccagcgcg ctggcgccag cgatgtcgac ttcggcgtgt
       61 cgctggtcat cctcggcgat gtgctgttct tcatgatgct tgtctggtcc accgccgggt
      121 tattgcgccg cgctgatgtg catcgacaac agacccagac agcgctcgcc ggggaacgca
      181 cgctgttgcg cgggctgatc gacaacctgc ccgatcacat tttcatcaag gacgcggaag
      241 gccgatacat caccgacaat atttcgcacg cccagttcgt cgggttgtcg tcgcccgagc
      301 aggtcgtcgg caaaaccgtc gctgattttt ttccgccgga ggtggcggcg aagttcgccg
      361 cggatgatgc cgcggtcgtt aactccgcca ggccgctcgt cagccgggtc gaagtcatca
      421 ccgagcacga cggctcgcgg cgctgggtgg caacgaccaa ggtgcccgtg cgcgacgccg
      481 ccggcggcgt caccgggttg gtctgcatca gtcgagacat taccgatcaa gtgcgcgctg
      541 acgagctcct gcgcgagcag aacgtgaagt tgcaagactt ggctcgatcc gagcgtgacg
      601 cccacgagac gctgaagcgc gcccagagcc agatggtcca gagcgagaag ctcgccggcc
      661 tcggccagct ggttgccggc gtggcgcacg agatcaacaa cccgctgtcg ttcgtcaaca
      721 acaacgtggc ggtgctgcag cgcgacgtgg gggcgatcgc gaagctgctg aaaatgtatc
      781 gccaggcgga cccgcttgtg caggcgggga acgcggagct gatgagcgaa attcgcgacc
      841 tgtgcgatgc catcgacatc gactacacgc tgaccagcat cgatgaactc acgatgcgct
      901 cgcgcgaggg gctgaagcgc atccagcaaa tcgtcaagga cctgcgcgat ttcgcccggc
      961 tcgacgagag cgacctgcac gaggtggacc tgaaccacgg cgtcgaatcc accgtcaaca
     1021 tcatccgcgg gcgagcgaaa tccaagcagg ttttgctgga actggacctg cacccgctgc
     1081 cgccggtcgc gtgcttcccg gcgaaggtca accaggtgat catgaacctc ctcgccaacg
     1141 ccatcgacgc ctgccgcgac ggtggcaagg tcacgatcag cacacacgca ctcgacaacg
     1201 gcgacggcgt gcgcgtcgag gtcgccgaca ccggcagcgg catcccgccc gacgtgcgcg
     1261 agcggatctt cgatccgttc ttcacgacca agaagcaggg cgaaggcacc gggctgggac
     1321 tgagcatcag ctacggcatc atccaggacc acggcgggac gattgaggtg gagtcgaagg
     1381 aaggcgaagg ttcaaggttt acggtgacgc tcccgctgca cgcgagcatc ccgtcgcgcc
     1441 gcaacaaaag cggttgatgg cgcgctcacc ccgacgccgg tgtctgaggg tactcccgaa
     1501 gacccgggtc ttcgggagta
//
LOCUS       DATIMH010000227         4117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3803164, whole genome shotgun sequence.
ACCESSION   DATIMH010000227 DATIMH010000000
VERSION     DATIMH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3803164"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1552)
                     /locus_tag="VLI90_03840"
     CDS             complement(185..1552)
                     /locus_tag="VLI90_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSV13362.1"
                     /translation="MIETLCSCGGAIRAAENTAGKVVPCGACGHAVRLAAAEAISPET
                     SAGDFDARFAITSGPESVGDTIALGGVPDIDIGKIAGRHIILAGGAMVSRAHAKLVRI
                     DFGPSRWKIVDTQSRNGVFVNGKRIAEQELNDGDIVQVGDYLLQYAVGFPAPDASFEP
                     QPESEAPSKARGRKIVAADVPCRKCQYNLRGLPADGKCPECGAPVRLSIHEPLIKFSD
                     PQWVDTLRRGINCIIFGVVAAVVSVIAGMASGALGSMLPAVLISLASSVLFIAGGWFL
                     TAPDPSGIGEDAYGTVRKLIRITLIIRIVSDVAEFGEQSGSVPREMRSLLVVIGFVAS
                     ISGVVGIVAMLNYLSKLAERIPDEYLAERAKFLMWAVGTCYGVFIAIGAIIAFAAASA
                     ARSGTGAVVGAMAGFGCIALIDGIALLIFMIRYLMFLGNLNTQLTEQSALAKHAWSAA
                     NAAAQ"
     gene            1760..3790
                     /locus_tag="VLI90_03845"
     CDS             1760..3790
                     /locus_tag="VLI90_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV13363.1"
                     /translation="MAATLGWGIIGTGAIAGTLANAIGKSQTGKLIAVASRARQQAET
                     FGATFNLDATKCYGNYESLLADPAVHAVYIATPHPLHATWAIKAAEARKHVLVEKPIA
                     VNQFQAQAIVEAAIANDVLLMEAFMYRSHPQTRRVVELLKERAIGDARVIHATFSFQA
                     GFSADSRLYSNALAGGAILDIGCYPMSMARLLAGAATGIDFADPIDVKSVGHLESTGV
                     DGYTLAVAKFPGDILAQLSAGVAINQENVVRIFGTDGRIFLSNPWVANRTAPDQGRII
                     VHRNGEKEPREITIDADVTSFTLEVDAFGDAVNSGRRQASAPAMTWADSLGNIRALDA
                     WRSQIGLTYESETPQGYPKVTVHDRPLRVRDKNKMKYGSVPHVSKRVSRLVMGVDNQS
                     ALPQAMVMFDTFFEAGGNAFDTAFVYGGGRHEQNLGHWMKIRGVRDDVAVIVKGAHTP
                     ECYPHAITRQLLISLDRLQTDRADLYLMHRDNPNVPVGEFVDVLNEHVRAGRFNAFGG
                     SNWSVARLTEANDYAKRKGLQGFTLMSNNFSLARMVSPVWGGCVAASDPDTRAWLTQT
                     QTTLLAWSSQARGFFTDRAHREQALNDEELNRCWYSEDNWQRRDRVIELAKRRGVLPI
                     NIALAYVLNQPFPTFPLIGPRTLEELRTSLPALNVELSAAEVRWLNLEAASA"
BASE COUNT          810 a         1309 c         1278 g          720 t
ORIGIN      
        1 ctcgatgatc cagaaagcat tacctagggc ggcgcgcgcg cgacgctgta gcgagcaaga
       61 acgtccccgt gtagccggcg agcttggtcg ccgtgtttta cgatcgaacg gaaatacggc
      121 gagcaagatt gccggttaca cgggctgcta tgcaagcgtt gattggtttt tatgaatggt
      181 tcgctcactg cgccgcggcg ttggcggcgg accaggcatg cttcgccaac gccgattgct
      241 cggtcagctg cgtgttcagg ttcccgagga acatcaggta gcggatcatg aagatcaaca
      301 gcgcgatgcc gtcgatgagg gcaatgcatc cgaagcctgc catggcgccc accaccgcgc
      361 cggtgccgga gcgagcggcg ctggcggcgg cgaaggcgat gatcgctccg atggcgatga
      421 acacgccgta gcacgtgccg accgcccaca tcaggaactt ggcgcgctca gccagatatt
      481 catccggaat gcgttcggcc aatttgctca ggtaattcag catcgcgacg atgccgacca
      541 cgccggagat cgacgcgacg aagccgatca ccacgagcag gcttcgcatc tcccgcggca
      601 cgctgcccga ttgttctccg aactccgcca cgtcgctgac gatgcgaatg atcagcgtga
      661 tgcgaatcag ctttcgcacg gtgccgtacg catcttcgcc aataccgctc ggatcaggcg
      721 cggtgaggaa ccatccaccg gcgatgaaca gcacgctgga agccagagaa atcagcaccg
      781 cgggcagcat gctgcctagc gcgcctgatg ccatgccggc aatcaccgat acaacggccg
      841 cgaccacgcc gaagatgatg cagttgatgc cgcggcgcag cgtgtccacc cattgcggat
      901 cggaaaactt gatgagtggt tcatggatgg acaggcgcac gggcgcgccg cactcggggc
      961 atttgccgtc agccggcagc ccgcgcaggt tgtattggca ctttcgacac ggcacgtcgg
     1021 ccgccacgat cttgcgaccg cgcgctttcg atggcgcttc gctttccggc tggggttcga
     1081 agctcgcgtc cggcgccgga aatccgactg catattgcag caggtaatcg ccgacctgca
     1141 cgatgtcgcc gtcgttcagt tcctgctcgg cgatgcgctt gccattgacg aagacgccgt
     1201 tgcggctctg cgtgtcgacg atcttccagc gcgacggacc gaagtcgatc cgcaccaact
     1261 tcgcatgcgc gcgcgagacc atcgcgccgc cggcaaggat gatgtgccgg ccggcgattt
     1321 tgccgatgtc gatgtccggc accccgccca atgcgatcgt atcgccgacg ctttcgggac
     1381 cagacgtgat ggcgaatcgc gcgtcgaaat cgcccgcgga ggtctcgggc gaaattgcct
     1441 ccgccgccgc cagacgaact gcgtgaccgc aggcgccgca aggtacgacc ttgcccgcgg
     1501 tgttctcggc cgcgcgaatc gcgccgccac atgaacagag tgtttcgatc atccccatgc
     1561 cccgtggaaa atgccctcac tgaattagat ggagcatcaa ggatatcaaa ggggcaacat
     1621 cagggcgaac aaaatcgccc tccctctccc cggtacgccg gggagagggc cgggtgaggg
     1681 gcagcgcgag aagtcgaacg cctctcgatc tcgatctcga tcatcgacgc gcatcgtctg
     1741 cccggataaa agtgccccca tggcagcaac cctcggatgg ggcatcatcg gaactggcgc
     1801 gatcgcaggc acgctggcga acgccattgg caaaagtcaa accggcaaat taatcgccgt
     1861 cgcaagccgg gcgcgccagc aggcggaaac gtttggcgca acgttcaacc tcgacgcgac
     1921 caaatgttat ggcaattacg aatcgctgct ggctgacccg gcggtgcacg cggtgtacat
     1981 cgccacgccg catccgctgc acgcgacgtg ggcgatcaag gcggccgagg cgcgcaagca
     2041 cgtgctggtc gagaagccga tcgcggtgaa tcagtttcaa gcgcaggcga tcgtcgaagc
     2101 cgccatcgcc aatgacgtgc tgctgatgga agcgttcatg tatcgcagcc acccgcaaac
     2161 ccggcgagtt gtcgagctgt tgaaggagcg cgcgattggc gacgcgcgag tcatccacgc
     2221 gacgttcagc ttccaggccg gctttagcgc cgatagccgg ttgtacagca acgcgctggc
     2281 cggtggggcg atcctcgaca tcggctgcta ccccatgtcg atggcccgcc tgctcgccgg
     2341 cgccgcgacc ggaatcgact tcgccgatcc gatcgacgtc aaatccgtcg gtcacctcga
     2401 atccaccggt gtcgatggtt acacgctcgc cgtcgccaaa ttccccggcg acatcctcgc
     2461 tcagctgtca gccggcgtgg cgatcaacca ggaaaacgtc gtccgcatct tcggcacaga
     2521 cgggcggatc ttcctgtcca atccctgggt cgccaatcgc accgcccccg atcagggccg
     2581 gatcatcgtg catcgcaacg gggagaagga gccgcgcgag atcacgatcg acgccgacgt
     2641 cacctcgttc acgcttgaag tggatgcgtt tggcgacgcc gtcaactccg gtcgacgcca
     2701 agccagcgcg ccggcgatga cgtgggcgga ttcgctcggc aacatccgcg cgctcgacgc
     2761 gtggcgaagc cagatcggtc tcacgtacga atccgaaacg ccgcagggat atccgaaggt
     2821 gactgtgcac gatcggccgc tgcgcgtccg cgataagaat aaaatgaagt acggctcggt
     2881 gccgcacgtg tcgaagcgcg tctcgcgcct ggtgatgggc gtcgacaatc agagcgcgct
     2941 gccgcaggcg atggtgatgt tcgacacctt cttcgaagcc ggcggcaacg cgttcgacac
     3001 cgcgttcgtc tacggtggcg gccggcacga gcagaacctg ggtcactgga tgaagattcg
     3061 tggtgtgcgc gacgacgtcg ccgtcatcgt caaaggcgcg cacacgcccg agtgttaccc
     3121 gcatgcgatc acgcggcagc tactgatcag cctcgatcgc ctgcagactg atcgcgcgga
     3181 tttgtacctg atgcaccgcg acaaccccaa tgtgccggtc ggtgaattcg tcgacgtgct
     3241 gaacgagcac gtgcgcgccg gccgattcaa tgctttcggc gggagcaact ggtcggtcgc
     3301 gcgcctcacc gaagccaacg attacgcgaa gcgcaagggc ctgcagggct tcacgctgat
     3361 gagcaacaac ttcagcctcg cccgcatggt cagccccgtg tggggcggct gcgtcgccgc
     3421 gtccgatccc gacacgcgcg cgtggctcac gcaaacgcaa acgacgctgt tggcgtggag
     3481 cagccaggcc cgcgggttct tcaccgatcg cgcccatcgc gagcaggcgc tgaacgacga
     3541 agaactgaac cgctgctggt attcggaaga caactggcaa cgacgcgacc gcgtgattga
     3601 gttagcaaag cgccgcggcg tgctgccgat taacatcgcg ctggcgtacg tactgaacca
     3661 gccgttcccc acgttccccc tgatcggccc gcggacgttg gaagaactgc gtaccagttt
     3721 gccggcgctg aacgttgaac tgtcagccgc ggaagtgcgg tggttgaacc tcgaagccgc
     3781 cagcgcctga acatccgcgc gctgccgctc gcgcgcgttc taaaatacgc aattccagaa
     3841 agtggtgaag ctcaccgtga catcggcctc cggcccatgc gtggcgctcc gtagcagcag
     3901 aggctatagc ttttcttaca gtaaatttga tgcctcacgc acgggccgga ggtccgtgtc
     3961 acggtaaggc gctaatacgc atctgaatta agctgcgcgc tcgatccacg gtgagcgggt
     4021 cagcgttttg agccgctcgt catcaaagct gttccacgca cgtttgatgg cttcgaacag
     4081 ctcatggtaa tccttgatgc ggttgctcag gtaatgg
//
LOCUS       DATIMH010000228         1598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4380003, whole genome shotgun sequence.
ACCESSION   DATIMH010000228 DATIMH010000000
VERSION     DATIMH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4380003"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VLI90_03850"
     CDS             complement(<1..922)
                     /locus_tag="VLI90_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HSV13364.1"
                     /translation="MEADYIVIGAGSAGCVLAARLSENPATKVLLLEAGGSDRNPWIH
                     IPLGYGKTIADPSVNWCYRTEPDLNGRRYYWPRGKVLGGSSSINGLVYIRGQSRDFDY
                     WRQLGNPGWSFADVLPYFKRAEDRIGEDGGELHGTGGPMAVSSADDDDPISEAFIAGA
                     VGLGFPRNHDFNGHAQDGAGYYHLTTRKGRRCSTAVGYLRPAMRRPNLRVVTHALTER
                     ILLDGKRATGVEWRVGNRIFRASATREVIVCAGALNSPQLLLLSGIGPAAHLAEQGIA
                     VQHDLPGVGQSLQDHYQARIVLKCTREITLN"
     gene            1259..>1598
                     /locus_tag="VLI90_03855"
     CDS             1259..>1598
                     /locus_tag="VLI90_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13365.1"
                     /translation="MAEAYICDYTRTPIGRYAGALRDVRTDDLAAMPIKALMARNPGV
                     DWEALDDCIMGCANQAGEDNRNVARMAVLLAGLPTTVPGGTVNRLCGSGLDAVGTAAR
                     AIKAGEAGLMI"
BASE COUNT          276 a          546 c          513 g          263 t
ORIGIN      
        1 cgttgagcgt gatctcacgc gtgcatttaa ggacgatgcg ggcctgataa tgatcctgca
       61 acgattgtcc gacaccgggc agatcgtgtt gcaccgcgat cccttgttcg gccagatgcg
      121 ccgccggccc gatcccggac agcagcaaca actgcggcga gttcagcgcc ccggcgcaaa
      181 cgatgacctc gcgcgtcgca ctggcgcgga agatgcggtt gccgacccgc cattccaccc
      241 ccgtcgcgcg tttgccgtcc agcaaaatcc gttcggtcaa tgcgtgcgtg acgacccgaa
      301 ggttgggccg gcgcatcgcc ggccgcaaat agccaacggc ggtcgagcat cgccgcccct
      361 tgcgcgtggt gagatgataa tatccggcgc cgtcctgcgc gtgcccgttg aagtcatggt
      421 tgcgagggaa accaaggccg accgcacccg cgatgaacgc ctcactgatg ggatcgtcgt
      481 catcggccga cgacacggcc atcggtccgc cggttccatg cagttccccg ccgtcctcgc
      541 cgatccggtc ctcggcgcgt ttgaaatacg gcagcacgtc cgcgaacgac catccgggat
      601 tgccgagttg ccgccagtaa tcgaagtccc tgctctggcc gcggatatag acgagcccgt
      661 tgatcgagga ggatccgcca agcactttgc cgcgcggcca gtaatagcgg cgcccgttca
      721 gatcgggctc cgttctgtag caccagttca cggacggatc ggcgatggtc ttgccatagc
      781 ccagaggaat atggatccac gggttgcggt cggacccgcc cgcctccagc aacagcacct
      841 tcgtcgcggg gttctcggac aggcgagcgg ccagaacgca accggcggag cccgctccga
      901 tgacgatgta gtcggcttcc attggttgca tgactgctcc cacacgcgcg gtgaaaacgg
      961 ctccacgcgc gtggtgagac aaaccccgtt ctcacccggc ggttccgatc caggctgcct
     1021 gaccgccgca aacggcgatg cgtcgccgga attcggtgtg gcatgacgat gcccgccgcc
     1081 ctcgcggacg tcgtcgatcg ccacgtcgtc cgcgatggta aaccgggaag ccactgctgg
     1141 cggaggtgtc gccccggccg ggtgaagcgt gccggtcgtg atgcgtctcg ccgaccgatc
     1201 ttggcccgca ccgcgcggcg tattacaaac ccggcaacct tcctgggagc aaacggacat
     1261 ggccgaagcc tatatctgcg actacacccg cacgccgatc gggcgttatg ccggcgccct
     1321 gcgcgatgtc cgcacggacg acctcgcggc gatgccgatc aaggcgctga tggcgcgcaa
     1381 tccgggcgtc gattgggaag cgctggacga ttgcatcatg ggctgcgcca atcaggccgg
     1441 tgaggataac cgcaacgtcg cccgcatggc cgtcctgctg gccggccttc ccacgacggt
     1501 gcccggcggc acggtgaacc gcctctgcgg ctccggcctc gacgccgtcg gcacggcggc
     1561 gcgcgccatc aaggccggcg aagcgggcct gatgatcg
//
LOCUS       DATIMH010000229         1692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2648578, whole genome shotgun sequence.
ACCESSION   DATIMH010000229 DATIMH010000000
VERSION     DATIMH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2648578"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VLI90_03860"
     CDS             complement(<1..246)
                     /locus_tag="VLI90_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13366.1"
                     /translation="MTSPVMQQIGTPLMTVQSYAEAFNCGDAVAMAALFAVPGHILDG
                     MAPHVWSGPSATIDWCRDALAEAEHLGISDFQMTLGTP"
     gene            complement(342..707)
                     /locus_tag="VLI90_03865"
     CDS             complement(342..707)
                     /locus_tag="VLI90_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HSV13367.1"
                     /translation="MPLSGSCHCGAVRVTVPEVAEWLGSCNCSICRRTAWLVAYYPDD
                     GQVRIEGKTEPYIWGDRMIALHHCPICACGTHWDSTGESYGRVGVNARLLDSFELRDG
                     RPLIEGWEVEVRYLDNADG"
     gene            876..1193
                     /locus_tag="VLI90_03870"
     CDS             876..1193
                     /locus_tag="VLI90_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13368.1"
                     /translation="MKEQSLEYYRQRARAECEAAFNATCPEARHAHAEMAKAYDHLAQ
                     IAELEQQGEIPPGKVTTMADALRDREEWELGGHAPGNVAEGQDQLASDDGLPPRNGSN
                     GLT"
     gene            complement(1205..>1692)
                     /locus_tag="VLI90_03875"
     CDS             complement(1205..>1692)
                     /locus_tag="VLI90_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13369.1"
                     /translation="TTSFQGGTPVPYEIKVDRTPDQKLVVHLLFSGRQGAEADIDLVP
                     QDGGNSTLLTNRIQADHAVLRPVLAGTDKAKLGYAPDWMFNLTFKPLLKQLAAQIEQG
                     QPASLTGQTQADAEAQWESNLSDEQRNQVAQWRQYDATRPTTDPNADADQYMKGTGGS
                     N"
BASE COUNT          304 a          542 c          528 g          318 t
ORIGIN      
        1 cggcgttccg agtgtcattt gaaaatcgct gatgccgaga tgctcggcct ccgcgagcgc
       61 atccctgcac cagtcgatcg ttgccgaagg acccgaccac acatgcggcg ccatgccgtc
      121 gaggatgtgg cctggcaccg caaagagcgc cgccatcgca accgcgtccc cgcaattgaa
      181 cgcctctgcg tagctttgga cggtcatcag cggcgtgccg atttgctgca tcacggggct
      241 ggtcatttgc tatgctcctg cgcatcccgc gacgggcttg gggcccatcg gcgcatacga
      301 ccatcgcgtt gcccctgact ttctgcagtt agcaaacgat cctagccgtc cgcattgtcg
      361 agatagcgca cctcgacctc ccagccctcg atgagcgggc gcccgtcgcg cagctcgaag
      421 ctgtccagaa gtcgcgcgtt gacgccaacg cggccatagc tttcgccggt gctgtcccag
      481 tgggtgccgc aggcgcagat ggggcagtgg tgaagcgcga tcatccggtc gccccagatg
      541 tagggctccg tctttccttc tatgcggacc tggccgtcat cagggtagta agcgaccagc
      601 caggcggtcc gcctgcagat gctgcaattg cacgacccca gccactcagc gacctccggc
      661 accgtcaccc gcacggcccc gcaatgacac gaacctgaaa gcggcatttg cgcaccttta
      721 cctgtcaacc tgccaccgtg cagtgtgagt atcatcgcag tcgcgcatac tccatggacg
      781 cagatcatcg cggtccaatg gcagctgacg gataatcgct tgtaattaaa ggccttaacc
      841 agccccctaa gagagatctc cggggggata tcgacttgaa agaacaatcg ctcgaatatt
      901 accgccagcg tgcccgtgcc gaatgtgagg ccgccttcaa cgcgacttgc ccggaggctc
      961 ggcatgccca tgcggaaatg gcgaaggctt acgaccatct cgcgcagatc gcggagctag
     1021 agcagcaggg tgagattcct cccggtaagg tgaccacgat ggccgacgcg ctccgcgacc
     1081 gtgaggagtg ggaactcggc ggacacgctc caggcaacgt ggcggaggga caagaccagc
     1141 tggcgtcaga tgacggtctc ccgccgcgca acgggtcgaa cgggctaacc tagcctgccg
     1201 gtgattagtt cgagccgccg gtgcctttca tatactgatc ggcgtcggca tttggatcgg
     1261 tcgtgggccg cgtggcgtcg tactgccgcc actgcgcaac ctggttgcgc tgctcgtcgg
     1321 agaggttcga ctcccattgc gcttcggcat ccgcctgcgt ctgtcccgtc aggctcgcag
     1381 gctgcccttg ctcgatctgt gccgcgagct gcttgagcag cggcttgaac gttaggttga
     1441 acatccagtc gggcgcatag ccgagcttcg ccttgtcggt cccggcgagc accgggcgga
     1501 gcacggcgtg atcggcctgg atgcggttgg tgagcaaagt gctgttgccg ccgtcctgcg
     1561 gaacgaggtc gatgtccgcc tcggccccct gccggccgga aaagagcagg tgaacgacga
     1621 gcttctggtc gggtgttcga tcgaccttga tctcgtaggg caccggcgtg ccaccctgga
     1681 aagaagtggt gc
//
LOCUS       DATIMH010000230         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4380391, whole genome shotgun sequence.
ACCESSION   DATIMH010000230 DATIMH010000000
VERSION     DATIMH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4380391"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="VLI90_03880"
     CDS             complement(<1..1597)
                     /locus_tag="VLI90_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSV13370.1"
                     /translation="MDARHAAWAALLASASAIAQQAPVRTPMDDAANDASAQLDRIVV
                     TARRRDEAVQDVPLSVTAVNGAELESRGIEDLGGLGAAVPNLTIYPARAFNSTITAYI
                     RGVGQSDPAWGFEPGVAVYVDDVYLARPQAALLDILDVQRIEVLRGPQGTLYGKNTIG
                     GAIKYVTRPVGDAFSGEATIALGDYDRRDFKAIINAPLSDTVRARIALGSFDRAGFGH
                     NLLTGEDVSAKHVQVARLTAQWLPRDDVDVRVAYDRYRDRSGMRGARRLEIDPFDPSR
                     TPPNDNGFDVQSGMADVDNADSENASVTVDWDLAAGWRLKSVTAHHSADTLGNVDFDT
                     LPLPISDLTRHIFDEQASQEFQLHRDSQRAHAVAGLYYFDGEADGRVNVNSNGQLFLR
                     STGSVKTRSAAVYGDLTLDLSDRFSVEGGLRYTSERKTGTVFNQGYTDATFTTPSGFV
                     AADFTHSKTFGAFSPRLSLSWHPTTDAMLYLQASRGFKSGSYNIRANTVFVPDSGRPY
                     ADETSTSYELGAKTQWLDGRVTLN"
     gene            complement(1902..2132)
                     /locus_tag="VLI90_03885"
     CDS             complement(1902..2132)
                     /locus_tag="VLI90_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13371.1"
                     /translation="MSRLLVPARLAPLSSFCVSITGNAGGQETGSRRRVCRGASMRVA
                     TLSGCRSLRTVRFLALRIRRSPWRDTTDRCRG"
BASE COUNT          390 a          745 c          801 g          376 t
ORIGIN      
        1 cgttgagcgt cacccgccca tccagccatt gcgtcttggc gcccagttcg taggaggtcg
       61 aggtttcatc cgcgtacggc cgtcccgaat cggggacgaa cacggtgttg gcgcggatgt
      121 tgtagctgcc gctcttgaag ccgcggctgg cctgcaggta cagcatcgcg tccgtggtcg
      181 ggtgccatga caggctcagc cgcggcgaga acgcgccgaa ggttttcgaa tgggtgaaat
      241 ccgcggcaac gaatccgctc ggcgtggtga acgtggcatc ggtgtagccc tggttgaaga
      301 ccgtgccggt tttgcgctcc gaggtgtagc gcaagccgcc ttccacgctg aacctgtcgc
      361 tgaggtccag ggtgagatcg ccgtagaccg cggcgctgcg ggtcttgacc gatcccgtgc
      421 tgcgcaggaa cagctgtccg ttgctgttca cgttgacccg gccatcggct tcgccgtcga
      481 agtaatacag cccggcgacg gcgtgggcgc gctgcgaatc ccggtgcaac tggaattcct
      541 gcgatgcctg ttcgtcgaag atgtgacggg tgagatcgga aatcggcaac ggcagcgtgt
      601 cgaaatcgac gttgcccagg gtgtcggcgc tgtggtgcgc ggtgacggat ttcaggcgcc
      661 agcccgcggc aagatcccag tccaccgtga ccgatgcgtt ttccgaatcc gcattgtcga
      721 catcggccat gccgctctgc acgtcgaaac cattgtcgtt gggcggcgtg cgcgacggat
      781 cgaacggatc gatctccagg cgcctggcgc cgcgcatgcc ggagcggtcg cgataacggt
      841 cgtacgccac gcgcacgtcg acatcatcgc ggggcagcca ctgtgcggtc aggcgcgcaa
      901 cctgcacgtg cttggcgctg acgtcttcgc ccgtgagcag gttgtggccg aacccggcgc
      961 gatcgaagct ccccagcgcg atgcgtgcac gcactgtgtc ggacagcggc gcattgatga
     1021 tcgccttgaa gtcgcggcgg tcgtagtcgc cgagcgcgat ggtcgcctcg ccggaaaatg
     1081 cgtcgcccac cggccgggtg acgtatttga tcgcgccgcc gatggtgttc ttgccataca
     1141 gcgtgccctg cggtccgcgc agcacttcga tgcgctgcac gtcgaggatg tcgagcagcg
     1201 cagcctgggg acgcgcgagg taaacatcgt ccacatagac cgcaacgccc ggttcaaacc
     1261 cccacgccgg atccgactgg cccacgccgc ggatgtacgc ggtgatcgtg ctgttgaagg
     1321 cccgggccgg gtagatcgtg agattgggga ccgccgcccc caacccgccc aagtcctcga
     1381 tgccgcggga ttccagctcg gcgccattga cggccgtgac cgacagcggc acgtcctgca
     1441 cggcttcgtc gcgccggcgc gcggtgacca cgatccggtc cagctgcgcc gacgcgtcat
     1501 tggcggcgtc gtccatcggg gtccgcacgg gcgcctgctg ggcgatggcg gacgcggagg
     1561 cgagcaaggc cgcccaagct gcatgccgcg cgtccaaggt tggcattccg tgtccccgaa
     1621 gttgtcgcgt catgccgctg gcggtctgca gtggtggact gcgccgcact gcggggttcc
     1681 agccttccag cgaatcggcg ccccgtttac agcccaacgt cgacggcgat tgtcggcggc
     1741 cccgatacgg ccggatccgc gcgcggcccg ttgacagtcg caaggcccgg gcgtaggttg
     1801 gatcccctgc gtcgccacaa catcttgtgg tgaacggggc caaggaagcc caagtgctgt
     1861 ggtgcgccgc agcgaggcac gcagggtggg ggcggcgtaa ttcagccgcg gcaacggtcg
     1921 gtggtgtctc gccaagggga tcggcggatg cgcagggcga ggaacctcac ggtccgcagg
     1981 gacctacatc cagacaacgt cgcgacgcgc atgctggcgc cgcggcacac ccgtcgccgc
     2041 gatccggtct cctgaccgcc cgcgttgccg gtgatcgaca cacagaacga cgacagcggc
     2101 gccagccgcg ctgggacaag gagacgggac acgatgagca cggtgcggtt ggaggcggtc
     2161 aagacggaca tgagccaggc gacgacatcg gacaagacgg gcgccgacaa ggcggccgat
     2221 agggaaatcc cgatgcagcc ggcctccacc gacatctggg acaagaaata ccgcctgaag
     2281 accaaggccg gcgacgccat cgatgccgac at
//
LOCUS       DATIMH010000231         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2938239, whole genome shotgun sequence.
ACCESSION   DATIMH010000231 DATIMH010000000
VERSION     DATIMH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2938239"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2547)
                     /locus_tag="VLI90_03890"
     CDS             complement(<1..2547)
                     /locus_tag="VLI90_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSV13372.1"
                     /translation="MSNSPLPLRFDFLPAWQAAALYLMLAIPVLWLGARSMTGLASFR
                     KWTAIAIRLDLLMFLVLLIAGARWEQTPKDLTVVVVRDVSRSTANVPSPNPAGLQMQV
                     TQYLREAARNKPAGDRIGAVSFDRLSMVDALPSGNPGTAGDTAAVRECADGTNVAQAL
                     RMAMAAMPADTMRRIVLLWDGNATTGDLGPAVDAAASLHIPIDVVSLYYNIKHEICLE
                     RLVAPAVRRQDEAFTLDVIVRSHNVARVDATLSLSDGGVCLQRTFPITLDPGANLIHV
                     PVSPLPAGAHHFHAAVQPVTPGSDGIRENNAADAVTLVRGPAKVLLVHGSETDDTTQL
                     REAMLRQGVTIDEQAEPALLPRTPADLETYDAVVLVNVPCGGPNGLDASQNLALARYV
                     KDLGGGLIAIGGTNTFGAAGWGGTELEKVLPINCQPPPHDPTTPGALVIAIDRSASMG
                     ERLRGSTRTKQELANESAALALRSLLPCDYAGVVAFNSTPQWIVPLCNGVEHARVDAA
                     IRSIASAGDTEIDPALMMACDALKQIPTAPGRAAGVRNVLLVTDGRSRDANLIGTVAA
                     MQRAGVTLSTVAIGDDVDQRRLDFLAWMGHGRSYRVTDPAMLPLTVICDARMVDASLI
                     RERPFTPRFTGVGDWSSGFAPLPPLRGMVMTWPKRSPAVRMSVVDDQGQPVLADWTIG
                     MGHAAVFTSDAGPRWAAAWIESPVFERFWAHALHRVLRPDPPVDVTARIVATSPDRAE
                     VIVESPAAAAGASTMMATIVVPDGQQPATSLRLIRSSDGAYRGEFATPDNGTYVAVVH
                     DADAASLTPAIYVASASAELSDLRSNEQAVRDIAQRTGGRLLPPFDPKANL"
BASE COUNT          466 a          901 c          897 g          405 t
ORIGIN      
        1 caaattcgcc ttcggatcga acggcggcag cagccgaccg ccggtgcgct gggcgatgtc
       61 gcgaactgcc tgctcgttgg atcgaagatc gcttagctcc gccgacgccg acgcgacata
      121 gatggcgggc gtcaggctgg ccgcgtcggc atcgtgaacg acggcgacgt acgtgccgtt
      181 gtccggcgtc gcgaattcgc cgcggtacgc gccgtcggat gagcggatca accgcaggct
      241 ggtcgccggt tgttggccat cgggcacaac gatcgtcgcc atcatggtcg aagcgccggc
      301 cgcggccgcg ggcgattcga cgatcacctc ggcgcgatcc ggactggtcg cgacgatgcg
      361 cgccgtaaca tccaccggtg gatccggccg caggacgcgg tgcagcgcgt gagcccaaaa
      421 ccgctcgaag accggcgact cgatccacgc ggccgcccag cgcgggccgg catcggacgt
      481 gaacaccgcg gcgtgtccca tgccgatcgt ccagtcagcc agcacgggct ggccctggtc
      541 atcgacgacc gacatgcgca ctgccgggga gcgcttcggc caggtcatca ccatgccgcg
      601 cagcggcggc agcggagcga agccgctgga ccaatcgccg acgcccgtga accgcggcgt
      661 gaacggccgc tcgcgaatga gcgaggcatc caccattctc gcatcgcata tcaccgtcag
      721 cggcagcatc gccggatcgg tcacgcggta cgatcggccg tgacccatcc acgcgaggaa
      781 gtcgagccgc cgctgatcca catcgtcgcc gatcgcgacc gtcgaaagcg tcaccccggc
      841 gcgctgcatc gcggcaacgg tgccaatcaa attcgcgtcg cggctgcgcc cgtccgtcac
      901 gagcaacaca tttcgaacgc cggcggcgcg accgggcgct gtagggattt gcttcagcgc
      961 atcgcatgcc atcatcagcg ctggatcaat ctcggtatcg ccggcgctgg cgatgctgcg
     1021 gatcgcggca tcgacgcgtg cgtgctcgac gccgttacac agtggcacga tccactgcgg
     1081 cgtcgaattg aacgcgacga caccggcgta atcgcagggc agcagcgatc gcagcgccag
     1141 cgccgccgac tcgttcgcca gttcctgctt ggtgcgcgtg gaaccgcgca gccgttcgcc
     1201 catggacgcc gaccgatcaa tggcgatcac cagcgccccc ggcgtcgtcg gatcatgcgg
     1261 cggtggctgg cagttgatcg ggagaacctt ctccaactca gtgccgcccc aaccggctgc
     1321 gccgaacgtg ttcgtgccgc cgatggcgat cagaccgccg cccaggtctt tgacgtatcg
     1381 cgccagcgcc aggttctgcg aagcgtccaa cccgttcggc ccgccgcagg ggacgttcac
     1441 cagcacgacg gcgtcatacg tttccaggtc ggccggtgtc cgcggcaata gcgccggctc
     1501 cgcctgttcg tcgatcgtga cgccctggcg aagcatcgcc tcgcgcagct gcgtcgtgtc
     1561 atccgtctcc gagccatgaa caagcagcac cttcgccggc ccgcgaacaa gcgttaccgc
     1621 gtcggcggcg ttgttctcgc gaatgccgtc gctgcccggc gtcaccggtt gcaccgcggc
     1681 gtggaaatgg tgcgcgccgg cgggcagcgg tgacaccggc acgtggatca ggttcgcgcc
     1741 ggggtcgagc gtgatcggaa acgttcgctg caggcaaacg ccgccatcgc tcagcgacaa
     1801 cgtggcatcg acgcgcgcga cgttgtgcga gcggacgatg acgtccaacg tgaacgcttc
     1861 atcctgtcgg cgaacggcgg gggcgaccag gcgctccaag cagatctcgt gcttgatgtt
     1921 gtagtagagt gagacgacgt cgatgggaat gtgcagcgag gcggccgcat cgacggcggg
     1981 gccgaggtcg ccggtggtgg cgttgccgtc ccacagcagc acgatccgcc gcatcgtatc
     2041 ggccggcatg gctgccatcg ccatgcgcag cgcctgcgcg acgttggtgc cgtcggcaca
     2101 ttcgcgcacc gcggcggtgt caccggccgt tccgggattg cccgagggca acgcgtcaac
     2161 catcgacagg cgatcgaagc tgaccgcgcc gatgcgatcg cccgcaggtt tgttccgcgc
     2221 cgcttcgcgc aggtactgcg tcacctgcat ctgcaacccc gccgggttag gcgacggcac
     2281 gttcgcggtc gatcggctca cgtcgcgaac cacgacgacc gtcagatctt tcggcgtctg
     2341 ctcccatcgc gccccggcga tcagcagcac caggaacatc aacaggtcga gtcgaatggc
     2401 aatcgcggtc catttgcgga aggaagcgag cccggtcatc gatcgcgcgc cgagccagag
     2461 cacgggaatc gcgagcatga ggtagagggc cgccgcttgc cacgccggca ggaagtcgaa
     2521 ccgcagcgga agcggcgaat tggacatgca agcaacaatt agacttcgcc gacagcggtg
     2581 gggtcaagga atatctgcgg tggggagggt gaaacctgag tacaccgggt gcagaacgca
     2641 ggtcacgagg agccgcgcac ggagcgtac
//
LOCUS       DATIMH010000232         6315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2649213, whole genome shotgun sequence.
ACCESSION   DATIMH010000232 DATIMH010000000
VERSION     DATIMH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2649213"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VLI90_03895"
     CDS             <1..1038
                     /locus_tag="VLI90_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV13373.1"
                     /translation="VASGTEGLTLGSITMSGNGVLDVVDGASASTLVTVTGVVGGAFT
                     LTKTGSGDMALNTVNTYSGDTTVSGGSLTIASAGSIASANVTVSAGATLSINGSLASS
                     VAISDSGVVNFAGNPGTSAFTRAVGTLNVQHGGSAVALDSAFAMQAGVSQITTLTFAD
                     TTAKLDLRNNELIVNNALPAVRTQIILGEIFTTTAGGVIGYKDLGSGQTEARYALRGD
                     ANLDGTVDVSDLGALATSYGNSTGQLWVQGDFNNDGSVDVADLGALASSYGVSLASGL
                     QAARLITAAAAATVASPASAASAIPRTALSNDAVRTTSYWIAGASPFGQNDRTVAWIF
                     GDSRRDKLRPN"
     gene            1415..2350
                     /locus_tag="VLI90_03900"
     CDS             1415..2350
                     /locus_tag="VLI90_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13374.1"
                     /translation="MLRRSLLVSAVIGFCTSSLFGGAVMFGGLGGHGPGSNSTNDGSL
                     VTIDQTTGDVTVVGHPAGVARLTGITFDSSGALFGSTLGSIPFPPPPAKSTSSLIQIN
                     PATGAIESTIGSITDGAGGAAISIADLAAQPGTGTLFGIRSPVDGLGGQGNLYTINKS
                     TGVAKLVGATGRFFASIAFAPNGTLYMTGADLGPNGAEVNPQLLTLDPANAKVKTSVT
                     TDGFFGALGVRPGDGAIFAGNGDGSQIFTLNAGTGAATALSHNTGTNFVGDLDFRPAT
                     NAIPLPPALWSGIIGMIALVGLASARMLRSAGTGY"
     gene            complement(2617..4662)
                     /locus_tag="VLI90_03905"
     CDS             complement(2617..4662)
                     /locus_tag="VLI90_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV13375.1"
                     /translation="MPASLRSSRRRYLDYRTRLKERRRRRDAAAKGDADIPAAGGHGQ
                     TDRKNRPRSRSFLTLLAQFWDLLRGYRRTVIIVLIAASLSTLLGLVPLYGTKIVFDNV
                     LNDHPLPARVPRWIHMPENRRQLLTFVAVAMVLLSAFSETVGLWGRWQMTRMTKRVQV
                     SVRKKVFDHAVRLPLHRVYDLKSGGVASILREDAGGVAELIFSMLYNPSRAIVQLITI
                     VVILAWTDWRLLLGSLALLPTVWVTHRTWIGRIRPIFRDIRSTRQQMDSHATEAFGGM
                     RVVRSFSRQQAEAATFTRNGHLMARQEIFAWWWMRGIDMTWSLLIPLASALLLYWGGN
                     RVLGDMEKVRAGLIAPKDALTVGDLVMFLSYLAALLGPIAALAGSATSLQNSLSGLDR
                     VLDLLTEPREMPSKPGAIVVDREHVTGRITLRDVSFAYSGSSTPVLHDVNLDVQPGEM
                     IALVGPSGAGKTTLCNLIARFYDPTAGAVMLDGVDLRDITADSYRRLLGIVEQDTFLF
                     DGTITENIAYGRRGATREQIEQAARLANAHDFIGALPDGYDSLIGERGVKLSGGQRQR
                     LTIARAILADPRILILDEATSNLDTESERLIQGSLQTLMAGRTSFVIAHRLSTIAHAS
                     RILVLENGRIVEQGRHEELMQASGRYRQMVDLQTSPPPPPRAEARVPRDEAFANSAN"
     gene            4862..5908
                     /locus_tag="VLI90_03910"
     CDS             4862..5908
                     /locus_tag="VLI90_03910"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HSV13376.1"
                     /translation="MSLYPLKFKPRFLEKIWGGRKLETVLGKSLPPEKLIGESWELYD
                     FPPGVIEGSGDEWTSAEIANGALAGRSLHWVVQEFGRDLMGDVALTGEHGQFPILIKF
                     LDAREDLSVQVHPDQEYAQSHPGAHLKSEAWYVMQHDAGARLLKGLVPGATRSDFEAA
                     TVVGGVDRLIKTIPAKVGDCHYLPSGTVHALGAGMLVAEVQTPSDTTFRLFDFNRIDA
                     TTGKPRKLHVNEALACIDFSGAPEPAQKRSHVAGFHTTVSQLVNSPYFKIEKVRFTEG
                     VEEPVPYDEPVVWMMLEGEAQVKVDGVKEPTRFAKGDTVLLPAAMKDPVIKTIKDCVW
                     LEVTFPTEANRPAV"
     gene            complement(5932..6144)
                     /locus_tag="VLI90_03915"
     CDS             complement(5932..6144)
                     /locus_tag="VLI90_03915"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HSV13377.1"
                     /translation="MKRKKKRRRSTDEGSYICPTCGEAIVVPLDRSGGSEQKYVEDCP
                     VCCNPNVIHVEFFDDGETPRIWADAE"
BASE COUNT         1146 a         1954 c         2092 g         1123 t
ORIGIN      
        1 gttgccagcg gcaccgaagg cttgacgctc gggtcgatca cgatgagcgg caatggcgtg
       61 ctcgacgtcg tcgatggcgc atcggccagc accctcgtga ccgtgaccgg cgtggtcggc
      121 ggggcgttca cgctgaccaa gaccggcagc ggagatatgg cgctgaacac ggtgaacacc
      181 tacagcggcg atacaaccgt cagcggcggg tcgctgacga ttgccagcgc cgggtcgatc
      241 gccagcgcga acgtgaccgt gagcgcgggc gcgacgctga gcatcaacgg ctcgctcgcc
      301 agcagcgtgg cgatcagcga tagcggcgtc gtcaacttcg ccggtaaccc cggcaccagc
      361 gccttcaccc gagccgtcgg cacgttgaac gttcagcacg gcgggtcggc cgtggcgctg
      421 gactcggcgt ttgcgatgca ggcgggggtc tcgcaaatca ccacgctgac cttcgctgac
      481 acgacggcaa agctcgatct gcggaacaac gagttgatcg tgaacaacgc gctgccggct
      541 gtgcgcaccc agatcatcct cggcgaaatc ttcaccacca ccgccggcgg cgtgatcgga
      601 tataaggacc tgggcagcgg gcagaccgaa gcgcgctacg cgctgcgcgg cgacgcgaac
      661 ctcgacggca ccgtcgatgt cagcgacctg ggcgcgctgg ccaccagtta cggcaacagc
      721 accggccagc tgtgggtcca aggggatttc aacaacgacg ggagcgtgga cgttgcggac
      781 ctgggcgcgc tggcgtccag ctacggcgtc agcctcgcca gtggcctgca ggccgcgcgg
      841 ctaataacgg cggcggcagc ggcgacggtc gcgagtcccg cgagcgcggc cagcgcaatt
      901 cctcgcaccg ctctgagcaa tgacgctgtt cgcacaacca gttattggat cgcgggcgca
      961 tcgccattcg ggcaaaacga cagaacggtc gcatggatct tcggcgactc gcgcagggac
     1021 aagctgcgcc cgaattgacg cgaccatgcc cgcacatgcg aacatgccgg cgctggcgcg
     1081 cggttggtcg cgaaagagat ggaacacaaa tcgcaggcgc agggggctcg gccaggcaga
     1141 gccccctgcg cctgcgcttt ttcctttcga ctgttcgatg ggtagcttgc cggttcgaca
     1201 atgctgctct tcttgccgtc actccttgct tgccgcgcct aagggaaatc cttattgccg
     1261 cattcccggt agcgcggcta actcatttgc agagcatctc gcatcgtcgt gggtgtagac
     1321 gcggtcatgc gggcgcgaaa cgactcgcgc gcgtgcgctc gctccgcttc gatcgcttcg
     1381 cctgcttttg aaaaccaaga agggacgggt tcatatgctg cgccgttcat tgctcgtttc
     1441 agcggtcatt ggtttctgca ccagcagcct gttcggcggc gcagtgatgt ttggcggcct
     1501 tggcggccac gggcccggat cgaactccac caacgatgga tcgctggtga cgatcgacca
     1561 aaccactggc gatgtcacgg tggttggcca tcccgcgggg gtggcgcggc tgactggaat
     1621 caccttcgat tccagcggcg cactgttcgg ctcgacgctg ggcagcattc cgtttccacc
     1681 gccgcccgcg aagtccacca gctcactgat ccagatcaat cccgcgaccg gcgcgattga
     1741 atcgacgatc gggtcaatca ccgacggcgc gggcggcgcg gcgatcagca tcgccgatct
     1801 cgcggcgcag ccgggcacgg ggacgctgtt cggcattcga tcgccggtcg atgggctggg
     1861 tggacagggg aatctttaca cgatcaacaa aagcaccggc gtcgccaagc tggtgggcgc
     1921 gaccggtcgt ttcttcgcca gcatcgcctt cgcgcccaac ggcacgctct atatgacggg
     1981 cgcggatctg ggtccgaacg gcgcggaggt caaccctcag ctgctcacgc tcgatccggc
     2041 caacgcgaag gtgaaaacca gcgtgacgac cgacggcttc ttcggcgcgc tgggcgtgcg
     2101 ccccggcgac ggcgcgatct tcgccggcaa cggtgacggg tcgcagatct tcacgctcaa
     2161 cgccggcacc ggcgcggcca ccgcgctgtc gcacaacacg ggcacgaact tcgtcggtga
     2221 cttggacttc cgtccagcaa ccaatgccat tccgctgccg ccggcgctgt ggtctggaat
     2281 cattggcatg attgcgctgg tcgggctggc atcggcaaga atgctgcgat cggctggtac
     2341 gggttattag tgggcgtgcc gattcaccag cgcggggctc agtctttccc cctctcccgg
     2401 tacgccggga gagggccggg gtgagggtga tttccgatga aaaaatgtcc aatgttcaaa
     2461 cgcgaaaaat gaacttccaa tgaccaagtc tctcgtggac attggcgatc ggcacttcat
     2521 tgggattttg gatttgctca ttggtcatta ccccgagtca ccctaaccct ctcccggaga
     2581 cctgcggtca gcagtaccgg gcgaggggac cagagctcaa tttgccgagt tcgcgaacgc
     2641 ctcatcacgc ggtacgcgcg cctctgctcg gggcggcggc gggggactcg tctgtaggtc
     2701 caccatctgc cgatatcgac cgctcgcctg catcagctcc tcatgccggc cttgctcgac
     2761 gatgcggccg ttttccagaa ccaggatccg gctcgcgtgc gcgatcgtgc tgagtcgatg
     2821 cgcgatcaca aaactggtcc ggcccgccat cagcgtttgc aggctgcctt ggatgagccg
     2881 ctcggattcg gtgtcgagat tgcttgtggc ttcgtcgagg atgaggatgc gcgggtccgc
     2941 taaaatcgcg cgggcgatcg ttaggcgctg acgctgcccg ccggaaagtt ttacgccgcg
     3001 ttcaccgatg agtgaatcgt agccgtccgg cagcgcgccg atgaaatcgt gcgcattcgc
     3061 cagccgggcg gcttgttcga tctgttcccg cgtcgccccg cggcggccgt aggcgatgtt
     3121 ctcggtgatc gtgccgtcga acaaaaacgt gtcctgttcg acgatgccga gcaggcgacg
     3181 gtaactatcg gcggtgatgt cgcgcaggtc cacgccgtcc agcatcaccg ccccggcggt
     3241 gggatcgtaa aagcgggcaa tcaggttgca cagggtggtc ttgccggcgc cgctggggcc
     3301 gaccagcgcg atcatctcgc ccggttgaac gtcgagattc acatcgtgca gcaccggcgt
     3361 gctcgacccg ctgtacgcga aggagacgtc tcgcagcgtg atgcgaccag tgacatgttc
     3421 gcgatcgacg acgatcgcgc cgggtttaga cggcatttcg cgcggctcgg tcagcaggtc
     3481 cagcacgcga tccaggccgg acagggagtt ctgcagcgag gtcgcgctgc cggccagcgc
     3541 ggcgatcggc ccgagcaacg ccgccaggta cgacaggaac atcaccagat cgcccacggt
     3601 tagcgcgtcc ttgggcgcga tcaatccagc cctcactttt tccatgtcgc ccagcacgcg
     3661 gttgccgccc cagtacagca gcagcgccga cgccagcggg atcagcagcg accacgtcat
     3721 gtcgatcccg cgcatccacc accacgcgaa gatttcctgc cgcgccatca ggtgcccgtt
     3781 acgcgtgaac gtggcggcct cggcttgttg acgcgagaac gatcgcacga cgcgcatgcc
     3841 gccgaacgcc tcggttgcgt gcgaatccat ctgctggcgc gtcgagcgga tgtcgcggaa
     3901 gatcggccga atgcggccga tccacgtgcg gtgcgtcacc cacaccgtcg gcagtagcgc
     3961 gagcgagccg aggagcagtc gccagtcggt ccatgcgagg atcaccacga tggtgatgag
     4021 ctgcacgatc gcccgcgacg ggttgtagag catggagaag atgagttccg ccacgccgcc
     4081 cgcgtcttcg cgcaggatcg acgcgacgcc gccggacttc aggtcgtaca cgcgatgcag
     4141 cggcagtcgc accgcgtggt cgaacacctt cttgcggacc gagacctgca cgcgcttggt
     4201 catgcgcgtc atctgccatc ggccccagag gccgaccgtt tcggagaatg ccgacagcaa
     4261 caccatcgcc accgcgacga acgtcagcag ttgccggcgg ttttccggca tgtgaatcca
     4321 ccgcggcacg cgcgccggca gcgggtgatc gttcagcacg ttgtcgaata cgatcttcgt
     4381 gccgtacagc gggaccaggc cgagcagcgt cgagaggctc gcggcgatca gaacgatgat
     4441 gacggtgcgg cggtaaccgc gcaataaatc ccagaattgc gcgagcaacg tgagaaacga
     4501 tcgcgaccgc ggacggttct tgcgatcggt ctgcccatgt ccaccggcgg ccgggatatc
     4561 ggcgtcaccc ttggcggcgg cgtcacggcg ccggcggcgc tccttcaagc gcgtgcgata
     4621 atcaaggtaa cggcgtcggc tggaacggag cgaagcaggc attggttgat actatcgtag
     4681 gcgcgatcgc ttggtatcga aagccacgcc gggctcgggg accgtacttg cgacgagcgg
     4741 gattcttctg cggcgcgggg agcaagcatc cgggtcgtcg cgggtaggct ccgagcccgg
     4801 cgtgggggag cgcctgacga aggctttgtc tggctccgct ggttttatat gatcgccggc
     4861 gatgtcactc tatccgctga aattcaaacc gcgatttctg gagaagatct ggggcgggcg
     4921 caagctcgaa acggtgcttg gcaaatcgtt gccgccagaa aagttgatcg gggaaagctg
     4981 ggagctttac gacttcccgc cgggcgtgat tgaaggctcg ggcgacgagt ggaccagcgc
     5041 ggagatcgcc aacggcgcgc tggcgggccg gtcgctgcat tgggtcgtgc aggagttcgg
     5101 ccgcgacctg atgggcgatg tcgcgctgac cggcgagcac gggcagttcc cgatcctgat
     5161 caaattcctg gacgctcgcg aagacctgtc ggtgcaggtg cacccggatc aggaatacgc
     5221 acagtcgcac ccgggtgcgc atttgaaaag cgaagcgtgg tacgtgatgc agcatgacgc
     5281 gggcgcgcgg ctgctgaaag gcctggtgcc cggcgcgacg cgcagcgact tcgaagccgc
     5341 caccgtcgtt ggcggtgtcg atcggttgat caagacgatt ccggcgaagg tgggtgattg
     5401 tcactatctg cccagcggca ccgttcacgc gctgggcgcg ggcatgctcg tggccgaagt
     5461 gcagacgccg agcgacacga cgtttcggct gttcgatttc aatcgtatcg acgcgacgac
     5521 cggcaaaccg cggaagctgc acgtgaacga ggcgctagcc tgcatcgatt tcagcggcgc
     5581 tccggagcca gcgcagaagc gcagtcacgt cgccgggttt cacacgacgg tgtcgcagct
     5641 ggtgaattcg ccgtatttca agatcgagaa ggtgcggttc acggagggcg tggaagaacc
     5701 ggtgccgtat gacgagcccg tggtgtggat gatgctcgaa ggcgaagcgc aggtgaaggt
     5761 ggatggcgtg aaggagccga cgaggtttgc caaaggcgac accgtgctgc tgccggcggc
     5821 gatgaaggac cccgtcatca aaacgattaa ggattgtgtg tggcttgaag tgacgttccc
     5881 caccgaagcg aatcgcccgg cggtgtgagt ggatgtcgct cccccgcgat atcactcggc
     5941 gtctgcccag atgcgcggcg tttcaccgtc gtcgaaaaat tcgacatgga tgacgttggg
     6001 attgcagcag acggggcaat cctcaacgta cttttgctcg gagccgccgg atcggtccag
     6061 cgggacgacg attgcttcgc cacaggtggg gcagatatag gagccttcat cggtcgagcg
     6121 gcggcgtttc ttcttgcgct tcatgtgccc attttacttg ctcggtcgct cgcgccagcg
     6181 gggcatcagt gacccagcac gcccacgtgt acgatcagat atgccaagct ggctatcagt
     6241 ccgatcaagc cgccgatttt gcccagcacg gcgcatgcgg acagatgata gatgttgccc
     6301 cagaagtagt ggcag
//
LOCUS       DATIMH010000233         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_340188, whole genome shotgun sequence.
ACCESSION   DATIMH010000233 DATIMH010000000
VERSION     DATIMH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340188"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VLI90_03920"
     CDS             complement(<1..434)
                     /locus_tag="VLI90_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HSV13378.1"
                     /translation="MNTVAIAGVGLIGGSFALALRKAGFQGTIIGVSSPATVQKALSL
                     GVIDEAMPLAEAVSAADLVYLAQPISQILETLDVLDTDALVTDAGSTKAVICDRARRL
                     KRFVGGHPMAGKESRGVEHADADLFRGRPYVLTMHEPQLETW"
     gene            complement(431..730)
                     /locus_tag="VLI90_03925"
     CDS             complement(431..730)
                     /locus_tag="VLI90_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HSV13379.1"
                     /translation="MTHEEAQHLLQNSRKRIDDIDVTLVDLLNRRAGVVEDIAAAKRV
                     LNKDVDDPGRREQVLAHARAQNAGPLSSDELTSIFERIIDKMSDHQVARLKTGRS"
     gene            complement(727..1512)
                     /gene="trpA"
                     /locus_tag="VLI90_03930"
     CDS             complement(727..1512)
                     /gene="trpA"
                     /locus_tag="VLI90_03930"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSV13380.1"
                     /translation="MQPTRIQKLFERTRKEGRAALIAYVTAGDPSPGRTPEVVAALER
                     GGVDLIELGVPFSDPIADGPVIQRAAARALAAGTTVPKVLDSARVIRQTSQMPILLFT
                     YLNPVLRYGLDKLARDAKSAGIDGCLLTDVSVEEAEPYVAAMRAAELDTVFLAAPTST
                     EHRLKLVAQYSTGFVYLVSRTGVTGEQVSLSQDLAPLIEKMRTVTNLPLAAGFGISTP
                     EQAGAVAKMADGVVVGSAIVRQVEKDPAGLESLARSLAERMKK"
     gene            complement(1512..>2626)
                     /gene="trpB"
                     /locus_tag="VLI90_03935"
     CDS             complement(1512..>2626)
                     /gene="trpB"
                     /locus_tag="VLI90_03935"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HSV13381.1"
                     /translation="EQAYRAARIDPTFQAELTDLLTHYAGRPTPLYYARRLSETLGGA
                     KIYIKREDLLHTGAHKINNCLGQALLARRMGKKRIIAETGAGQHGVATATVCALFGLE
                     CVVYMGEEDMRRQRLNVFRMRLLGATVVSVSNGSRTLKDAISEAMRDWVTNVHTTHYL
                     LGSALGAHPYPLMVRDFHRVIGDETRAEILNREGRLPDSVLACVGGGSNAIGIFHAFI
                     GDENVKLIGIEAGGRSLQLGEHAARFSGGAPGVLHGAKSYLLQDADGQAALTHSVSAG
                     LDYVLIGPEHAWLHDRNRAEYTSCSDADALAAAKKLARTEGIIPALESAHAVHETIRR
                     APLEPGKLFVVNLSGRGDKDTDIYRENFPELDAAGK"
BASE COUNT          499 a          814 c          785 g          528 t
ORIGIN      
        1 atccaggttt cgagttgagg ctcgtgcatg gtcagaacgt acggccggcc gcgaaacagg
       61 tccgcatcgg catgctccac cccgcgagat tccttgcccg ccatggggtg accgcctacg
      121 aaccgcttga gcctccgagc acgatcgcag atgaccgcct ttgtgctgcc cgcgtctgtg
      181 accaaggcgt ccgtgtccag cacatcgagc gtttcgagaa tctggctgat cggctgtgcg
      241 agataaacca gatccgctgc cgacaccgct tcggccagcg gcatggcttc atctatgaca
      301 ccgagagaca gggctttctg aacggttgcc ggtgaactga ccccaatgat cgttccctga
      361 aatccagcct tgcggagcgc caaggcaaat gagcccccaa tgagcccgac gccggcaatg
      421 gcaaccgtgt tcacgaccgt ccggttttga ggcgtgccac ctgatggtcg ctcattttat
      481 cgataatgcg ctcgaagata ctggtcaatt cgtcactcga cagaggaccg gcgttctgag
      541 ccctcgcgtg ggcgagcacc tgttcacggc ggcccggatc gtcgacgtct ttgttgagca
      601 ctctcttggc tgcagcgata tcctcgacca cacccgcacg gcgattcagc aggtcgacca
      661 gagtgacgtc gatatcgtcg atgcgcttcc ggctgttttg cagtagatgc tgtgcttcct
      721 catgcgtcat tttttcattc tctccgcgag cgaccgcgct agcgactcaa gaccggccgg
      781 gtccttctcg acctgccgta cgatcgcgct gccgacaaca actccgtccg ccatcttggc
      841 gacagcgcct gcttgctcgg gcgtcgagat cccaaatccg gcagcgagcg gaaggttcgt
      901 gacggttcgc attttctcga tcagcggcgc cagatcctgc gacagcgaca cttgttcgcc
      961 ggtgaccccg gtcctcgaaa caagatacac gaaaccggtg gaatactgcg cgaccagctt
     1021 caaacggtgc tccgtcgaag tcggcgcggc gagaaaaacg gtatctagct cggcggcgcg
     1081 catcgcggcg acatagggct cggcctcctc gacgctgacg tcggtaagca ggcacccatc
     1141 aatgccggcg ctctttgcgt cgcgggcgag tttatccagg ccgtagcgca gcacagggtt
     1201 caagtatgtg aacaacaaga tcggcatctg cgacgtttgc ctgatcaccc gggcgctatc
     1261 cagcaccttt ggaacggtgg tgccggcagc tagtgcccgt gcggcggccc gctggatcac
     1321 cggcccatcc gcaatgggat ctgagaacgg aacgcccaac tcgatcagat cgacgcctcc
     1381 gcgttctaac gccgcgacaa cttcgggggt gcgcccaggt gaaggatcgc cggcggtgac
     1441 gtaggctatg agcgctgcgc ggccctcctt gcgagtacgt tcaaacagct tctggatcct
     1501 ggtgggctgc atcactttcc tgccgcgtcc agctccggaa agttttcgcg gtagatgtcg
     1561 gtatctttgt cgccgcggcc cgaaagattg acgacaaaca actttccggg ctccaaaggg
     1621 gcacggcgaa tggtctcgtg cacggcatgc gccgattcga gcgccgggat gattccctcc
     1681 gtgcgagcca gctttttggc tgccgccaag gcgtcggcat cgctgcacga agtgtattcg
     1741 gcacggttcc gatcgtgaag ccacgcgtgt tcgggaccga tcaggacata gtcgaggccc
     1801 gccgaaacgg aatgcgtcag cgcagcctgg ccatcggcat cctggagtag ataactcttg
     1861 gccccatgga gcacgccggg agcaccgcca gagaaacgcg cggcatgttc accaagctgc
     1921 aggctgcggc cgcccgcttc aatgccgatc agcttcacgt tttcatcgcc gataaacgcg
     1981 tggaatatgc cgatggcgtt cgagcctccc cccacgcagg cgagaactga atccggcaat
     2041 cgtccttcac ggttgagaat ttcagcgcga gtttcatcgc caatcactcg gtggaaatcg
     2101 cgcaccataa gcggataggg atgggctccc agcgctgaac cgagcagata gtgggttgtg
     2161 tgaacgttag tgacccaatc gcgcatggct tcgctgatcg cgtccttgag tgtccggctg
     2221 ccgttcgaaa cagagaccac cgtagcgccc aaaagccgca tgcggaagac gttcaggcgc
     2281 tggcgtcgca tgtcttcttc acccatgtag accacgcatt ccaggccgaa cagtgcgcac
     2341 acggtggccg tggcgactcc gtgctgtccc gcgccggtct ccgcgatgat gcgcttcttt
     2401 cccatacggc gtgcgagcaa cgcctggccg aggcagttgt taatcttgtg tgcaccggtg
     2461 tgcagcaggt cttcgcgctt gatgtagatt ttggctccgc cgagtgtttc gctgagacga
     2521 cgcgcgtaat agagcggcgt gggtcgaccg gcgtagtgcg tcaacagatc ggtcagctct
     2581 gcttgaaatg taggatcgat tcgagccgcg cggtaagcct gctcca
//
LOCUS       DATIMH010000234         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_630344, whole genome shotgun sequence.
ACCESSION   DATIMH010000234 DATIMH010000000
VERSION     DATIMH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630344"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..417)
                     /locus_tag="VLI90_03940"
     CDS             complement(106..417)
                     /locus_tag="VLI90_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13382.1"
                     /translation="MSLFTPDALHEVESGSVIPDAAVGGALDGVIGVLRQESFDAGYR
                     RAVSDLLAEYLLITEEHLHDLPKPDPALRALLRSFEEQLTRFAQARLELEGFVEGGLG
                     I"
     gene            complement(669..1475)
                     /locus_tag="VLI90_03945"
     CDS             complement(669..1475)
                     /locus_tag="VLI90_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HSV13383.1"
                     /translation="MRIKIGFDLAFGISTPTPMLLMLYVHPSRQADLESAEILKLEPD
                     IPVETFIDVYGNKVGRIVAPPGDLKIHHSAVVHDSGKHDIPGTEALQHPVNQLLPHIL
                     PYLFSSRYCEVDRMADIAWSLFGQTPLGWPRVMAVQKWVHEHIQFGYEHARKNKTAYD
                     AYVEKRGVCRDYMHLSITFLRALNIPARYVTGYLGDIGVAFNPAPMDFSAWFEVFLGG
                     QWWTCDARHNEPRIGRILMATGRDATDVALTTCFGPTTLNRFEVVTEEVT"
     gene            1676..2650
                     /locus_tag="VLI90_03950"
     CDS             1676..2650
                     /locus_tag="VLI90_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13384.1"
                     /translation="MFGNRLGWGISAVIVLFAAFLAKLIYSAGLPTPPTGWVQKAVQP
                     LTAPAGTDAVVPGGMNEARDAGELYRRLIDDYEMNTAAYAAIAAAKELDPAAIAARPG
                     LQALLDATHCQTMTLFANKPEEIINYDAEKPALEALREAAKAATRTALLLGTRDPAAA
                     AKVYEAVFALGAKLYHERVVFDELSKGEDLMGTAAAALQRLAERTKDAERAKALQQFN
                     TDRLNDYEAKVKPVWDVVSSADEGIIAGYAGDIFVLAQDRTIEKMWRVEATLKLGRML
                     YMAGRRGDQLAAKRVLREMSADTSEDPAVRTAADQARNLTIQQYRTLR"
     gene            complement(2716..3021)
                     /locus_tag="VLI90_03955"
     CDS             complement(2716..3021)
                     /locus_tag="VLI90_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13385.1"
                     /translation="MAKAAGAKLRALGAVSSSTNWAERAAVCEGCPLRVVRGGVSYCG
                     TPFLQKIDREPAVDGCGCPTRDKAKSPGEHCPLDARQRPAMAIGSACTCKWCALTHL"
     gene            complement(3102..>3865)
                     /locus_tag="VLI90_03960"
     CDS             complement(3102..>3865)
                     /locus_tag="VLI90_03960"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HSV13386.1"
                     /translation="YWTEPQLIDEIRGLWRRTMQGFIAPGPDGTPPALLVEKTPGHAL
                     FMDVIMKVLPEARFVHVIRDSRSVAASLLAAKKDDWAKMWAPTKARDAALTWYQHVTA
                     ARKFGKTLPPEKYMEVFYEDFHANPAANLTRLLDYLNLPLPAARVNEIVAEQSFEKQK
                     AIGGTPFARLGKFENAKITEPKGFFRSGKADGWRQELSWRQKLTVWRFTRRLMASCGY
                     KWGGRQDGFVPLENSNTATTPGNVPAAPAPANAHS"
BASE COUNT          720 a         1206 c         1252 g          687 t
ORIGIN      
        1 ctcaccccgg ccctctccca cgagtacgtg ggagaggggg aaaggcgcgc acgcgcttta
       61 gcaaccgcgc gctcgctcag taggccggct tcaggcgcat cactgttaga tcccgagccc
      121 gccttcgacg aagccttcca actccagccg cgcctgcgcg aagcgcgtca gttgttcctc
      181 gaacgaccgc agcagcgcgc gcagcgccgg gtccggcttg ggcaggtcgt gcaggtgctc
      241 ctcggtgatc agcagatact ccgcgagcag atcgctgacg gcccggcgat agccggcgtc
      301 gaatgattcc tgccgcagca cgccaatcac gccatccaac gcgccgccca cggcggcgtc
      361 ggggatgacc gatccggatt caacttcgtg caacgcatcg ggtgtgaaca acgacatagg
      421 tgcgcctacc tgtttgtgcg ggacaatctt gtgtggaagc ggccgaacgg gcgaggcagg
      481 tcaacagcgg cacatcgcaa ccgccggggc ggtggcagtc atttgcgcaa acgtggcggg
      541 tgggaaactc atggtgggga agtgtatgga cccggtggac gctgtcaacc ataattctgc
      601 aattcctacc gttttggtag aaagtgttac atttgagaca aaatgttacg tttggagcaa
      661 tgttcgcgtt acgtaacctc ctccgtcacc acttcgaagc gattgagcgt cgtcggaccg
      721 aagcaagtcg ttaaagcaac gtcagttgca tcgcgaccgg tggccatgag aatccggccg
      781 atgcgcggct cgttgtgtcg tgcgtcgcag gtccaccatt ggccgccgag gaagacttcg
      841 aaccacgcgg agaagtccat cggggccggg ttgaaggcga cgccgatgtc gcccaggtac
      901 cccgtgacgt agcgggcggg gatgttgagg gctcggagga acgtgatgct gaggtgcatg
      961 tagtcgcggc agacgccgcg cttttcgacg taggcgtcgt acgccgtctt gttcttccgg
     1021 gcgtgttcgt agccgaactg gatgtgctca tgcacccact tctgcacggc catgacgcgc
     1081 ggccagccga gcggcgtttg gccaaacaag ctccaggcga tgtcggccat gcgatcgact
     1141 tcgcaatatc gactggaaaa cagatacggc aggatgtgcg gcagcagctg gttcaccgga
     1201 tgctgcaggg cctcggtgcc ggggatgtcg tgcttgccgg agtcgtggac gacggccgag
     1261 tgatggatct tcaaatcccc cggcggggcg acgattcggc cgaccttgtt gccgtagacg
     1321 tcgatgaacg tctcgacggg aatgtccggt tccagcttca ggatctcagc cgattccaag
     1381 tccgcctgcc gcgacgggtg gacgtacaac atcagcagca tcggcgtggg ggtggatatg
     1441 ccgaaggcga ggtcgaagcc gatcttaatg cgcattgcag gcgatctttc ggggctgcgc
     1501 gccggttggt tcgtggatac gctaactgaa gcggcgcgga accgtcaacg cgcgaagccg
     1561 catgcgcaaa gagccgcgcg cgaagtaatc gcgattttac aacgcccgcg gacttcgcgc
     1621 acggcttctt gcgcacgatt ccctttcggt caaccgcgct ctattctcat ctcccatgtt
     1681 cgggaaccgc ctgggctggg gcatttcggc cgtcatcgtt ctctttgccg cctttttagc
     1741 gaagttgatt tacagcgccg gcctgcccac gccgcctacc ggatgggtgc agaaagcggt
     1801 gcaaccgctg acagcgccgg cgggaacgga tgcggtggtg cccggcggga tgaacgaagc
     1861 gcgcgacgcg ggcgagctgt atcgccgcct catcgatgac tacgagatga acaccgccgc
     1921 gtacgcggcg atcgcggcgg cgaaggagct ggaccccgcg gccatcgccg cccggccggg
     1981 tttgcaggcg ctgctggatg cgacgcattg ccagacgatg acgcttttcg ccaacaagcc
     2041 cgaagagatc atcaattacg atgcggaaaa accagcgctg gaagcactgc gcgaagccgc
     2101 caaggcggcg acgcgcacgg cattgttgct cggcacgcgc gaccccgccg ccgcggcgaa
     2161 ggtgtatgaa gcggtcttcg cgctgggtgc gaagctgtat cacgagcggg tggtgttcga
     2221 cgagttgtcc aaaggcgaag acctgatggg caccgccgcc gccgcgttgc agcggctggc
     2281 ggagcgaacc aaggacgccg agcgggcgaa ggcgctgcag cagttcaaca ccgaccggct
     2341 gaacgattac gaggcgaagg tgaaaccagt gtgggacgtc gtcagcagcg cggacgaagg
     2401 catcatcgcg ggttacgccg gcgacatctt cgtgctggcg caagatcgga cgatcgaaaa
     2461 gatgtggcgg gtggaagcga cgctgaagct gggccggatg ctatacatgg ccggccgccg
     2521 gggcgaccag ctggcggcga aacgcgtgct gcgcgagatg tccgctgata catcggaaga
     2581 ccccgcggtc cgaaccgcgg cggatcaggc gcggaatctc acgatccagc agtatcgaac
     2641 gctgcgctga ttgaaggcga atatcgaaca cgccgggttc ggagagtact cgcgacgacc
     2701 cggatcgttc gcgcgctaga gatgagtcag cgcacaccac ttgcacgtgc acgccgaacc
     2761 gatcgccatc gccgggcgtt gtcgggcatc cagcggacaa tgctcccccg gcgacttcgc
     2821 cttatcgcgc gtaggacatc cgcagccatc cactgcaggc tcgcgatcga tcttctgcag
     2881 gaacggcgtg ccgcagtacg agacgccgcc gcggacgacg cgcagcggac aaccttcgca
     2941 gacggccgct cgctcggccc aatttgtgga tgatgacacc gccccgagcg cgcgcagttt
     3001 cgcccccgcg gccttggcca tcgctttggt caattcgcgg aatgggctag acgtgcaatt
     3061 ccagcaagcc atgctggaag agttatcggt cgttggctcg attaagagtg agcattggct
     3121 ggcgccggag ccgccggcac attgcccggc gtggtggccg tgttgctgtt ctcaagcggc
     3181 acgaacccgt cctgcctgcc gccccacttg taaccgcacg acgccatcaa ccgccgggtg
     3241 aaccgccaga cggtcagctt ctgccgccag ctcagctcct gccgccaacc gtcggctttg
     3301 ccgctgcgaa agaacccttt gggctcggtg atcttcgcgt tttcaaactt cccaagccgc
     3361 gcaaagggtg tcccgccgat cgccttttgc ttctcgaacg attgctcagc cacgatctcg
     3421 ttcacgcgcg ccgccggcaa cggcaggttc aggtaatcca gcaaccgcgt caggttcgcc
     3481 gcggggttgg cgtggaagtc ttcataaaag acctccatgt acttctccgg cggcagggtc
     3541 ttgccgaact tccgcgcggc ggtcacgtgt tgataccacg tcagcgctgc atcgcgcgct
     3601 ttcgtcggcg cccacatctt ggcccagtca tccttcttcg ccgcgagcag tgaggcggcg
     3661 acggaccggc tgtcgcggat cacatgcacg aagcgcgcct ccggcagcac cttcatgatg
     3721 acgtccatga acagcgcgtg ccccggcgtc ttctcgacca gcagcgcagg cggcgtgccg
     3781 tcgggaccgg gcgcgatgaa cccctgcatc gttcgccgcc acagcccgcg gatctcgtcg
     3841 atcaattgcg gctcagtcca ataat
//
LOCUS       DATIMH010000235         1754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3804429, whole genome shotgun sequence.
ACCESSION   DATIMH010000235 DATIMH010000000
VERSION     DATIMH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3804429"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /gene="ilvA"
                     /locus_tag="VLI90_03965"
     CDS             complement(<1..1127)
                     /gene="ilvA"
                     /locus_tag="VLI90_03965"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HSV13387.1"
                     /translation="MTPVTIDSIRAAHARIKDTIYRSPCTYSLALSRLCDAEIFCKLD
                     HLQMTGSFKERGARNKLLQLSAEQQCRGVIAASAGNHALALAYHGSLLSVPVTVVMPK
                     WAPLVKVANCRSLGANVILFGDSYDEAKHQATALGEERGLTIVSGFDDPDIIAGQGTL
                     ALEVLQDVPDADAIIVPVGGGGLIAGVAVAVKALRPATRIIGVEPTNAPTLHESLKRG
                     EATKVPTRPTLADGLAVAQMGELCFELIRPALDDLVMVDEPQIAQAMLRLLELEKMVV
                     EGAGAVTLAAAQIRKSDLAGKKVVLCLTGGNVDVTMISKVIDRGLAADGRLCRITAFA
                     GDRPGSLAHLTRVLADTGANVLEVAHDRHFSPADVLVVGIT"
     gene            1319..>1754
                     /locus_tag="VLI90_03970"
     CDS             1319..>1754
                     /locus_tag="VLI90_03970"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HSV13388.1"
                     /translation="MARLFAFIVIASVFLATTQLDCFAASSVEQWDVFDLQLNGSPEG
                     NPFIDVNISARFSNGADTVSVPGFYDGDGVYRIRFAPPRRGEWSYVTQSNRPELDGQR
                     GSVTATAATGDNHGPVRVRDTFHFAYADGAPYFECGTTCYAWT"
BASE COUNT          295 a          581 c          561 g          317 t
ORIGIN      
        1 caggtgatgc cgacgaccag cacgtcggcg ggggaaaagt ggcggtcgtg cgccacttcc
       61 agcacgttgg cgccggtgtc cgccagcaca cgcgtcagat gagcgagcga ccccgggcga
      121 tcgcccgcga acgcggtgat gcggcagagc cgcccgtcag cagcgaggcc gcgatcgatc
      181 actttcgaaa tcatggtcac gtccacattg ccaccggtca ggcagaggac gaccttcttc
      241 ccagccaagt cggacttgcg aatctgcgcc gccgccagcg tgacggcacc ggcgccttcg
      301 acgaccatct tttccagctc cagcagccgc agcatcgcct gcgcgatctg cggctcatcg
      361 accatcacca ggtcatccag cgccggccga atcagctcga agcaaagctc gcccatctgt
      421 gcgaccgcca ggccgtcggc cagcgtcggc cgggttggca cctttgttgc ctcgccgcgc
      481 ttcagcgatt cgtgcagcgt cggcgcgttg gtcggctcga cgccgatgat gcgcgtcgcc
      541 ggccgcagcg ctttaacggc gacagcaacg ccggcgatca acccgccgcc gccgaccggc
      601 acgatgatcg cgtcggcgtc gggcacgtct tggaggacct ccagcgcaag cgtcccctgg
      661 ccggcgatga tgtccgggtc gtcgaagccg gagacgatcg tgaggccgcg ttcctcgcca
      721 agcgcggtcg cctggtgctt ggcttcatcg taggagtcgc cgaagaggat cacgttcgcg
      781 ccaagcgatc ggcagttggc caccttcacc agcggggccc acttgggcat gacgacggtg
      841 acggggaccg acaacaggct gccgtggtac gccagcgcca gcgcgtgatt gcccgcggat
      901 gccgcgatga cgccgcggca ctgctgctcg gcggacagtt ggagcagctt gtttcgcgcg
      961 ccgcgctctt taaacgaccc ggtcatctgc agatggtcga gcttgcagaa gatctccgca
     1021 tcgcacagcc ggctcagcgc cagcgagtaa gtgcaggggc tgcggtagat ggtgtctttg
     1081 atgcgcgcgt gagcggcgcg gatcgaatcg atggtgacgg gcgtcatgcg cgatcatcgt
     1141 accatgacga gcgtgtcgca gtggttatca tgccttggcg ccatcgcagc cgcggggctt
     1201 tagcccgcgg actgtccgtc gctcgaatcg ttctcgttac gtcaacggtc cgcgggcgat
     1261 agcgccgcga ttgcgattgt ggatccgagc cgattcttcg ttccaactag agatcttcat
     1321 ggcacgtctc ttcgctttca tcgtcatcgc cagtgtattc ctcgcaacga cgcaacttga
     1381 ctgcttcgcg gcttcgtcag tcgaacagtg ggacgtcttc gaccttcagc tcaacggctc
     1441 ccccgaaggc aatccgttta tcgacgtgaa catctccgcg cggttcagca acggcgccga
     1501 caccgtcagc gtccccggtt tctatgatgg cgatggcgtc tatcgaatcc gcttcgcgcc
     1561 accgcggcgg ggggaatgga gctacgtcac gcagagcaat cgcccggaac tggacggcca
     1621 gcgcggcagc gtgaccgcca ccgcggcaac cggcgataac catgggccgg tgcgcgtgcg
     1681 cgatacgttt cacttcgcct atgccgacgg cgcgccttac tttgaatgcg gcacgacgtg
     1741 ttatgcgtgg actc
//
LOCUS       DATIMH010000236         5327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_340457, whole genome shotgun sequence.
ACCESSION   DATIMH010000236 DATIMH010000000
VERSION     DATIMH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340457"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(581..2332)
                     /locus_tag="VLI90_03975"
     CDS             complement(581..2332)
                     /locus_tag="VLI90_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HSV13389.1"
                     /translation="MLTRRRFLRRSLEAGLAAAAPIGLGAPLSGCARAISAPAGSGDV
                     LVNHVGFTPAAAKFCLLAGNNATPFSVLDRSSSKVVQQGTLAVQHGDLGDFLVGDFSD
                     LEQAGQYEVRVGDRTAAPFSISPDVCSDGIAKCVGYFARQRCGNSQSGHHAPCHLDDG
                     RRSDNGQHQDATGGWHDACDLRKWVDATIYGMIGLSRVLDLSSSHGTDRDRIIDEMRW
                     GDGYFHRMQEPDGFVMDYCGGDDGNRYTDNQIGSGDDRTIHVEPCILPAQFHFVAAQA
                     AMARHVRDSDPDYAKACESAAERCLDWCVKNRSPGAATSLAAGIIACVELHRTTGDDR
                     LKDRAAEFMGRLLALQVTTADGDSPTGFFNAAPDKLEPSREIQHGNLPLIAMCEAIEQ
                     FKNHEKVGNWRQALGSHCEYLSGMASRSVFGTVPYGLYSGGDPGSHRRIGRYWYRWFM
                     PRRGETAAADWWVGVNAHLASNGVGLARAARVLQDPKLAVLAQRQLDWVLGVNPFNAS
                     WVTDVGRNQPKLYRSGQFAPPTPLIAGGVMNGIGGDDEDRPVLEPGSWNTCEYWTPMV
                     AYVMWLMAELQNAAVPV"
     gene            2794..3990
                     /locus_tag="VLI90_03980"
     CDS             2794..3990
                     /locus_tag="VLI90_03980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV13390.1"
                     /translation="MSRKLSVVHIINSFQFGGAERMLCNLLLNTDMDRFEPSVVSLID
                     DMTVAAPIIDAGIPITVVGMRPGIPDPRGIARLARHLWRLKPAVIQTWMDHSNLIGGI
                     AARLVSRTPLVWGIHHSNHIPSLTKKSTLATVWACKMFSRRVPTRIVCCSEHARGMYA
                     QRGFADDKLMVIPNGFDVSAFRPDPAARANLRRELDVHPQTPLVGLIARYDPFKDHET
                     FLRAAAKLKRIRPDVHYVLCGHNVDSNNSVLMAMIGSLGIGEQCHLIGARTDVPRVLA
                     GLDLAAQSSVSEAFPLVLGEAMACGVPCVATDVGDSRLIVGDTGKIVPARDPDALAAG
                     MGEMLAMRSSALQQLGITARTRVREMFDLKSVTRRYESLYTDLASAAGRAAPIEMQSR
                     EIVATS"
     gene            4051..>5327
                     /locus_tag="VLI90_03985"
     CDS             4051..>5327
                     /locus_tag="VLI90_03985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV13391.1"
                     /translation="MSVADSMILNAPAPDAARESDGARRPWRVLMIVESAAGGTGRHV
                     LDLCEGFIARGCDAHVIYSTGRVDRMFLNRLAKIPALHQQAIRMRTAPHPSDVGVVRA
                     VKRYMREHGPFDVIHGHSSKGGAIARLAAFGTGIPAFYTLHGLIMMDPGLPRWKWLMY
                     YSAEMAMTWRTQRIIAVSPEEQRAARKLGFGHDRVTMVPNGVGPLGGATRKIARQTMG
                     VAEDQIVIGFIGRLVEQKAPHILIEAFANTLKVTPNARLAIVGAGPLEREMHDLATRL
                     GAADKIIWLGERDARGVIAGFDLFALSSRKEGLPYVVLEAMVTGLPVVATNKAGVEIL
                     IDPGVSGEVVPADDAKAFGDALIRLTSDPARLAAQSKAAGERAKRFTVDAMVEGTIQA
                     YEGALSPSPGTPGAGRGEGDFERRAPLENSNHP"
BASE COUNT         1005 a         1845 c         1531 g          946 t
ORIGIN      
        1 gatcaccgcc cagcggcggc gccacagcac gtgcagcaac ggcgccggcg gcaccacacc
       61 gctgccgcca ccgttgacgg acgagccgtg gaaatgatcc gtcatgctcg aggcgtaacg
      121 gacgctggat tctctgcgaa gcaacgtatg cattctcagt gtttctcgcg gaccagacgc
      181 gggcgaccgc aacgacagac aaaccgcgac catcgcggcg agcgcgccgg gtgaaccggt
      241 acacacgctg tcgacaaacg cgaatcaacc tgtcaacgaa cgccgcgcgc caagacgcgg
      301 ttaaaactga cacccactgc ggtacaaaaa cgactatccc tgcccacacc acatccgcta
      361 gcaccggcgg tcaaacagca actcaagcgg cacccactgt gcccgctgga gggggaatac
      421 tacctcatgt tcagtcccca tgactgcctc tgccgcgcga ttgggatgtg ccgagcgcga
      481 acaaggatac gcccggaaag ttacgactga agctgcccaa attcaagtgc aatccatttc
      541 ttaacttggg taacttgcac tgtctttaac gttcaatccc ttaaaccggc accgcagcgt
      601 tctgcaattc ggccatcaac cacatgacat acgccaccat cggcgtccaa tactcgcagg
      661 tattccagct gcccggctcc aaaaccgggc gatcttcgtc atctccgccg atcccgttca
      721 tcacaccgcc cgcaattaac ggcgttggcg gcgcgaactg cccgcttctg tagagcttcg
      781 gttggttccg gccaacatcc gtcacccagc tggcgttgaa cggattgacg cccaggaccc
      841 aatccagctg ccgctgcgcc agcaccgcca gcttcggatc ctgcagaacc ctcgccgccc
      901 gcgccaagcc gactccattc gaagccaagt gggcattcac acccacccac caatccgcgg
      961 ccgcggtttc accacggcgc ggcatgaacc atcgatacca gtatcgacca atccggcgat
     1021 gacttccggg atccccgccg gaatagagcc cataagggac cgtcccaaaa acggaccggc
     1081 tggccatgcc cgataagtat tcgcagtggg aacccaaagc ctgccgccaa ttacccacct
     1141 tctcatgatt tttaaattgc tcgatcgcct cacacatcgc gatgagcgga aggttgccgt
     1201 gctgaatctc gcgcgatggc tcgagcttgt ccggcgccgc gttgaaaaaa ccggtgggac
     1261 tgtcgccatc ggcggtggtg acttgaagcg ccagcaatcg ccccatgaac tccgccgctc
     1321 ggtcctttaa ccgatcgtca ccggtcgttc gatgcagctc gacgcacgcg atgatccccg
     1381 ccgccagcga tgtcgcggca cccggtgatc ggttcttcac gcaccagtcc aggcatcgct
     1441 cggccgccga ctcgcacgcc ttggcgtaat ctggatcgct gtcgcgcacg tgccgggcca
     1501 tcgcggcctg tgccgcgacg aagtgaaact gcgccggcag gatgcacggc tcgacgtgaa
     1561 ttgtccggtc gtccccgctg ccgatttggt tgtcggtgta gcgattccca tcgtccccgc
     1621 cgcagtagtc catgacgaat ccgtccggct cctgcatgcg gtgaaagtag ccatcgcccc
     1681 accgcatctc atcgatgatg cgatcgcgat ctgtcccgtg agatgacgaa agatccagca
     1741 cgcgcgacag gccgatcatg ccgtagatcg ttgcatcaac ccacttgcgc aggtcgcacg
     1801 catcgtgcca accaccggtg gcatcctgat gctgcccatt gtcgcttcgc cggccgtcat
     1861 ctaagtgaca aggggcgtga tgaccgctct gactgttccc gcaacgttgc ctggcgaagt
     1921 agccgacgca tttggcgatg ccgtcgctgc acacatcagg cgagatcgaa aagggcgctg
     1981 ccgtacggtc accaacgcgc acctcgtatt ggccggcctg ttcaagatcg ctgaaatcgc
     2041 cgacaagaaa atcacctaaa tcgccgtgct gcaccgcgag cgttccctgt tgaaccacct
     2101 ttgatgaact gcgatctaaa acgctgaacg gcgtcgcgtt attccccgcc agcaggcaga
     2161 acttcgccgc cgccggggta aagccgacat gattgaccag cacatccccg ctgcccgccg
     2221 gggcggaaat agcgcgggcg catccgctca atggcgcccc cagcccgatc ggcgccgccg
     2281 ccgccagccc cgcctccagc gagcgtctca agaaccgccg acgtgtcagc atgtcaccgc
     2341 gcctccgcgt taccggacgg gccaaatcat accggctctc gctcgccgcg catcacatat
     2401 agaacggtat cgacgcgtcc ctcgcatgcc gcgcaatcac agtcgtcaca gtccctacac
     2461 cttcacaggg actacagcag cgcagcgcgc gagtgcgcca ttttgacttt gtggttgcgt
     2521 tcccctccgg cgcggttata tcctatgact gtcccgtggg cggttcagtg ggtgaacggc
     2581 cagcggacat gcagtgggtg aactgggaca tctcgctttg gagtgatcgc ctataactgg
     2641 tcttgcccgt tttggcgccg gcggcgcagt ggcaaaatcc ttaccccgcg ttcaaacgcg
     2701 cgggctgcgc gcgatcgtta tccagatttg tttagggcgt ccccccatcc cgctgccgtt
     2761 tcgagtcgat agtcagggga aggcagtaaa ctcatgagca ggaagctttc agtcgttcac
     2821 atcatcaaca gttttcagtt cggcggagcc gagcggatgc tttgcaatct gctgttgaac
     2881 accgacatgg atcggttcga gccgtcggtc gtctcgctga tcgatgacat gaccgtggcc
     2941 gccccgatca tcgacgcggg catcccgatc accgtcgtcg gcatgcggcc ggggattccc
     3001 gacccgcgcg gcatcgcacg cctcgcccgc catctgtggc gcctgaagcc agcggtcatt
     3061 cagacttgga tggaccactc gaacttgatc ggtggcatcg cggcccgatt ggtgtcgcgc
     3121 acaccgctgg tttggggcat ccaccattca aaccacattc cgtcgctgac gaaaaagtcc
     3181 acgctggcca ccgtgtgggc gtgcaagatg ttttcccgcc gggtgccgac gcgcatcgtc
     3241 tgctgctcgg aacacgcccg cggcatgtat gcccagcgcg gcttcgcgga cgacaagctg
     3301 atggtgatcc ccaacgggtt cgatgtcagc gcgttccgcc cggaccccgc cgcgcgagcc
     3361 aaccttcgcc gcgagctgga cgttcacccg caaacgccgc tggtaggact catcgcgcgt
     3421 tacgatccgt tcaaggatca cgagacgttc ctgcgcgctg ccgcgaagct gaagcgcatc
     3481 cgcccggacg tgcactatgt cctgtgcggc cacaatgtcg attcgaacaa ctcggtgctg
     3541 atggcgatga tcggctcgct cggcatcggt gaacaatgtc atctgatcgg cgcgcgcacc
     3601 gatgtgccgc gcgtgctggc tggcctcgat ctggccgctc aatcatccgt cagcgaagcg
     3661 ttcccgctgg tacttggcga agcgatggcg tgcggcgtgc cgtgcgtcgc caccgacgtc
     3721 ggtgactccc gcctgatcgt cggcgacacg ggcaagatcg tccccgcgcg cgatcccgat
     3781 gcactcgctg ccggcatggg tgaaatgctc gcgatgcgca gcagcgccct gcagcaactg
     3841 ggcatcaccg cccgcacccg cgtgcgcgag atgttcgacc tgaaatcggt cacgcgccgc
     3901 tacgaatcgc tctacaccga tctcgccagc gccgccggtc gcgccgcgcc gatcgaaatg
     3961 caatcccgcg agatcgttgc caccagctaa aaagccacgg ctctgccgtg ggactcttaa
     4021 ccgcgacacc aggagaagga gatcccagcc gtgtccgttg ccgattcgat gatcctcaac
     4081 gcccccgcac ccgacgccgc gcgcgaatca gacggcgccc gccgcccatg gcgcgtgctc
     4141 atgatcgtcg aatccgccgc cggtggcacc ggccgccacg tgctcgacct ctgcgaaggc
     4201 ttcatcgccc gcggctgcga tgcccacgtc atctactcca ccggccgggt cgatcggatg
     4261 ttcctcaatc gccttgcgaa gatccccgcg ttgcatcagc aggcgatccg catgcgcacc
     4321 gctccgcacc ccagcgacgt cggtgtcgtg cgcgcggtca agcgctacat gcgcgagcac
     4381 ggtcccttcg acgtcatcca cggccacagt tccaagggcg gcgcgatcgc gcgcctcgcc
     4441 gcgttcggca ccggcatccc cgccttctac acgctgcacg gcctgatcat gatggacccc
     4501 ggcctgccgc ggtggaaatg gctcatgtat tactcggccg agatggcgat gacttggcgc
     4561 acgcagcgca tcatcgccgt gtcacccgaa gaacaacgcg ccgcgcgaaa gctcggcttc
     4621 ggtcacgatc gcgtgacgat ggtccccaac ggcgtcggtc cgctcggtgg cgcgacgcgc
     4681 aagatcgccc gccagaccat gggcgtcgcg gaggatcaaa tcgtgattgg tttcatcggc
     4741 cggcttgtcg aacaaaaggc tccgcacatc ctgattgagg ccttcgccaa cacgctgaag
     4801 gtcacgccga acgcgcggct cgccatcgtc ggcgctggcc cgctcgaacg cgagatgcac
     4861 gatctggcaa cccgcctggg cgcagccgac aagatcatct ggctcggcga gcgcgacgcc
     4921 cgcggtgtca tcgcggggtt cgatctgttc gccctctcca gccgcaagga aggcttgccg
     4981 tacgtcgtgc tcgaagcgat ggtcaccggc ctgcccgtcg tcgccactaa caaagccggc
     5041 gtggaaatcc tgatcgaccc cggcgtcagc ggtgaagtcg tccccgccga cgacgccaag
     5101 gccttcggcg acgcgctgat ccgactgacg tccgatccgg cgcgcctcgc cgctcaatcc
     5161 aaagccgccg gtgagcgcgc caagcgattc acggtcgacg cgatggtcga aggcaccatt
     5221 caggcgtacg aaggtgcact gtccccctct ccgggtactc cgggagcggg ccggggtgag
     5281 ggtgattttg aacgtcgggc gcctctggaa aattcaaatc accctca
//
LOCUS       DATIMH010000237         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_52100, whole genome shotgun sequence.
ACCESSION   DATIMH010000237 DATIMH010000000
VERSION     DATIMH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52100"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VLI90_03990"
     CDS             <1..456
                     /locus_tag="VLI90_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13392.1"
                     /translation="TFEKKHGALASGVAATLREDLRRSVIKSLRSTVIEPEHRFFLAL
                     LMNAPTRDDLFSLVAQRARGQAPVSVVLRWVEELMEISDAGVTILDAAFPETLEVETQ
                     AQPNLFLAAFRHFVKRDKKLPAIMRDLTARDVNELRAAFADSALSLLTV"
     gene            602..>1506
                     /locus_tag="VLI90_03995"
     CDS             602..>1506
                     /locus_tag="VLI90_03995"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HSV13393.1"
                     /translation="MAYLPIEDHGLIGNMHTAALVGRNGSIDWLCLPHFDSPSIFAAI
                     LDDKKGGYFQIAPVDEKATRKQLYWPETNVLITRFFLADGVIEVIDYMPVGVKRGQPG
                     FRQIVRRVEAIRGTVPVRVDCCPAFNYARDPHTTDIHDRGAVFSSATLTVELVTAHPL
                     TMKSGACGVVAQLSLRQGEKATFVLRQLDEKHKGGPVTEEHEQELFNRTVDFWHKWLA
                     KCTYTGRWREMVYRSALVLKLLTFEPTGALVAAPTCSLPEAIPGPRNWDYRYTWIRDA
                     AFSLYALLRIGFHEEAGAFMSWIEAR"
BASE COUNT          275 a          459 c          498 g          274 t
ORIGIN      
        1 acgttcgaga aaaagcatgg cgcgctcgcc agcggcgtcg cagcaacact gagagaagac
       61 ctgcggcgca gcgtcatcaa gagcttgcgc agcacggtca tcgagcccga gcatcggttc
      121 ttcctggcac tgctgatgaa cgctccgacg cgcgacgatc tgttctcgct tgtggcgcag
      181 cgcgctcgcg ggcaagcgcc tgtttctgtc gtcctgcgct gggtggaaga gttgatggaa
      241 atatcggatg cgggcgtcac gatccttgat gcggcgtttc ccgaaacgct tgaagtcgaa
      301 acccaggcgc agccgaacct gttcctggcg gcgttccggc atttcgtgaa gcgcgacaaa
      361 aagcttccgg cgattatgcg tgacctgacc gcgcgagacg tgaacgaatt acgggccgcc
      421 ttcgcagact ccgctctgag ccttttgacc gtttagcctc tcagcaagca atcgcctgcg
      481 cgcggtccgc cggcggcggg gtgcactttt gcccgttccg atggggagcg ggggcagata
      541 gtttaggctc gcgcctgtgt gtggcggccc gagcgagcgg ggccgcgcgc ggtcgtgaga
      601 tatggcttac ctccccatcg aagatcacgg cctcatcggg aacatgcaca ccgccgcgct
      661 cgtcgggcga aacgggtcga tcgactggct ttgcctgccg cacttcgatt cgccgagcat
      721 cttcgcggcg atcctggacg acaaaaaagg cggatacttt cagatcgcgc cggtggacga
      781 gaaggcgacg cgcaagcagc tgtattggcc ggaaacgaac gtgctgatca cgcgattttt
      841 cctggcggac ggcgtgatcg aggtgatcga ttacatgccg gtgggcgtga agcgcggaca
      901 gccggggttt cgccagatcg tccgccgggt ggaagcaatc cgcggcaccg tgcccgtgcg
      961 cgtggactgc tgcccggcgt tcaactacgc gcgcgatccg cacacgaccg acatccacga
     1021 tcgcggcgcg gtcttcagca gcgcgacgct gaccgtcgag ctggtcacag cgcacccgct
     1081 gacgatgaag tccggcgcct gcggcgtcgt tgcgcagctg tcccttcgac aaggcgagaa
     1141 ggcgacgttc gtccttcgcc agctggacga aaagcacaag ggcgggccgg tcaccgaaga
     1201 acacgaacag gaactattca accgcacggt ggacttctgg cacaagtggc tggccaagtg
     1261 cacgtacacc gggcggtggc gcgagatggt atatcgatcg gcacttgtcc tgaagctttt
     1321 gacgtttgaa ccgaccggcg cgctggtggc ggcgccgacg tgcagcctgc ccgaggcgat
     1381 tcccggcccg cggaattggg actatcgcta cacgtggatt cgcgacgccg ctttttcgct
     1441 gtacgcgctg ctgcgcatcg gcttccatga agaagccggc gcgttcatga gctggatcga
     1501 agcgcg
//
LOCUS       DATIMH010000238         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1208327, whole genome shotgun sequence.
ACCESSION   DATIMH010000238 DATIMH010000000
VERSION     DATIMH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208327"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /gene="dprA"
                     /locus_tag="VLI90_04000"
     CDS             complement(<1..1138)
                     /gene="dprA"
                     /locus_tag="VLI90_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HSV13394.1"
                     /translation="MALCHWLQLALTDGIGPILARRLIETCGGVEAACAADTRTLQQI
                     EGIGSHKSGNILASLRAAAGEVDAELERAAAAGAAVICPDDEVYPALLNSIPDPPLVL
                     YVKGQLEPRDLNAVAIVGSRKCSFYGREQGERFAALLAGAGVTVISGGARGIDSASHR
                     GALSHPHGRTIAVQGCGVNIAYPPENAPLFEQIASRGAVVSEYPIDSPPVPENFPRRN
                     RIVSGMSRGVLVIEADERSGALITARQACDDHGRPVFALPGRVDNRMSAGPHMLIRDG
                     ATLVTKLEDILDSLGPLPQQVMESMPESLKEEVLTPPAQPSPREAPAGIDLELLSEGQ
                     RQIIAHLDGEPVSVDQIIERSGLDASAVMRELTFLSLKGVVRRVG"
     gene            complement(1159..1923)
                     /locus_tag="VLI90_04005"
     CDS             complement(1159..1923)
                     /locus_tag="VLI90_04005"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSV13395.1"
                     /translation="MPKSTLDILFQDDDFVAVNKPAGLAVIPGRGEAEGETVLERLAM
                     ELNLPASGAADPRIRVVHRLDKDTSGVMLFAKHVAAQRHVSHQFQNNTTSKEYLALVV
                     GRPSQSAGEIDAKLAPHPSSKLRMAVIRHGGRPARTQWRVEQSFRQYTLLRVFPKTGK
                     THQIRVHLKHIGLPLAVDPLYNPTPPGTPVGLFLSSFKRGYRSTRGEQERPLIARLTL
                     HAERLRFQDLGGREIDLVAPLPKDFRAALNMLARHG"
     gene            complement(1956..>2620)
                     /locus_tag="VLI90_04010"
     CDS             complement(1956..>2620)
                     /locus_tag="VLI90_04010"
                     /inference="COORDINATES: protein motif:HMM:NF016611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV13396.1"
                     /translation="FLNELRASVENYLHALELDPNDARSSANLGAVYLSLDDVDNALK
                     YLERATTLDPKSWYAWLNLGLARDAKGNYKEAEAAYRRAQELDSSQPSVLFNLASNLV
                     TQGRPDEAIAIFKQLLEREPNNARYHKRYADALVVANRPDDADKEYRESLRVDSRYYP
                     AMNELGLLLITRYKREAELDDDKKKSALDFWRQSLAVNPDQPRIKALIAEWGPHDQSG
                     KT"
BASE COUNT          433 a          886 c          819 g          482 t
ORIGIN      
        1 ccccaacccg gcggacgacg cccttcagcg acaggaacgt cagctcccgc atcaccgctg
       61 acgcgtccaa tccgctgcgc tcgatgatct gatccaccga cacgggctcg ccgtcgagat
      121 gcgcgatgat ctgtcgctgt ccctcgctca acaattccag atcgatgccc gccggcgctt
      181 cccgcggcga cggttgcgcc ggcggcgtga ggacttcttc tttaagcgac tcgggcatac
      241 tctccatcac ttgctgcggc agcggcccga gactatcgag aatatcttcc aacttcgtca
      301 ccagcgtcgc gccgtcacgg atcagcatgt ggggacctgc cgacatgcgg ttgtccacgc
      361 ggcccggcag cgcgaagacg gggcgaccat gatcgtcgca ggcttgtcgc gcggtgatga
      421 gcgcgccgct gcgttcgtcc gcttcgatga ccagcacgcc gcgggacatg ccgctgacga
      481 tgcggtttcg ccgcgggaag ttctcgggca ccggcgggct gtcgatgggg tattcgctga
      541 cgaccgcgcc gcgcgaggcg atttgttcga acagcggcgc gttctccggc ggataggcaa
      601 tgttcacgcc gcaaccctgg acggcgattg tccggccgtg gggatgactg agcgcgccgc
      661 ggtgtgacgc cgagtcaatt ccgcgagcgc cgccagagat cacggtcaca ccggcgccgg
      721 cgagcaacgc ggcgaagcgc tcgccctgct cgcgtccgta aaaactgcac ttgcggctgc
      781 cgacgatcgc caccgcgttc aaatcgcgcg gctcgagttg acccttcacg tacagcacca
      841 gcggtgggtc cgggatgctg ttcaacaacg ccgggtaaac ctcgtcatcc gggcaaatga
      901 ccgccgcgcc cgcggcggcg gcacgctcca actcggcatc gacttcaccc gccgccgcgc
      961 gaagagaggc aaggatgttt ccagatttgt gggagccaat gccctcgatc tgctgaagcg
     1021 ttcgcgtgtc cgcagcgcac gcggcctcga cgccgccgca ggtctcgatc aatcggcgcg
     1081 ccaggatcgg gccgatgcca tcggtcagcg cgagttgaag ccagtggcaa agggccatga
     1141 cgcgtgctta tatcgcgctc aaccgtggcg ggcgagcatg ttcagcgccg cgcggaaatc
     1201 cttcggcagc ggcgcgacca ggtcgatctc ccggcccccg aggtcttgaa accgcaaacg
     1261 ctcggcgtgc agcgtgaggc gcgcgatcag cggccgctct tgttcaccgc gcgtcgagcg
     1321 atagccgcgc ttgaacgatg agaggaacaa tcccaccggc gtccccggcg gcgtcgggtt
     1381 gtacaggggg tcgaccgcca gcggcaggcc gatgtgcttc aggtgcacgc ggatctggtg
     1441 cgttttgccg gtcttaggga acacgcgcag cagcgtgtat tggcggaagg attgctcgac
     1501 gcgccactgc gtgcgcgccg gtcggccacc gtggcggatg accgccatgc gcagcttgga
     1561 actcggatgc ggggcgagct tcgcgtcgat ctcgccggcc gattggctcg gtcgccccac
     1621 caccagcgcc agatattcct tgctcgtcgt gttgttctga aactggtgtg acacgtggcg
     1681 ctgcgccgcc acgtgcttgg caaacagcat cacgccgctg gtgtctttgt cgagccgatg
     1741 cacgacgcga atccgcgggt ccgcagcccc cgatgccgga agattcagct ccatcgccag
     1801 ccgctccagc accgtctccc cttcggcttc cccgcggccg ggaatcaccg ccagccccgc
     1861 gggtttgttc acggcgacga agtcgtcgtc ctgaaagaga atgtccagcg tagatttggg
     1921 cacgcgcgcg agtgcgatga ggtccggaag cagcgttagg ttttcccgct ctgatcgtgc
     1981 ggcccccact cggcgatcag cgccttgatc cgcggctggt ccgggttcac cgctaaactc
     2041 tgccgccaga aatccagtgc cgacttcttc ttgtcgtcgt ccagttccgc ctcgcgcttg
     2101 taccgcgtaa tgagcagcag ccccagctcg ttcattgccg ggtaatagcg cgagtccacg
     2161 cgcagcgact cgcgatattc cttgtccgcg tcatccgggc gattcgccac caccagcgcg
     2221 tcggcatatc gcttgtgata tcgcgcgttg ttcggctcgc gctcgagcag ttgcttgaag
     2281 atcgcaatcg cctcgtccgg ccgaccctgc gtcacgagat tgctcgccag gttgaacagc
     2341 accgacggct ggctcgaatc cagttcctgc gcccggcgat acgccgcctc tgcttccttg
     2401 taatttccct tggcgtcgcg cgccaggccc aggttcagcc aggcgtacca cgacttcgga
     2461 tccagcgtcg tcgcgcgttc caggtatttg agtgcgttat ccacatcgtc gagcgacagg
     2521 tagaccgcgc caagattggc agatgaacgc gcatcgttcg ggtccaactc cagcgcgtgc
     2581 aggtaattct ccaccgacgc ccgtaactcg ttcaaaaact
//
LOCUS       DATIMH010000239         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2072882, whole genome shotgun sequence.
ACCESSION   DATIMH010000239 DATIMH010000000
VERSION     DATIMH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072882"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1281
                     /locus_tag="VLI90_04015"
     CDS             52..1281
                     /locus_tag="VLI90_04015"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF006803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HSV13397.1"
                     /translation="MSFASIADCLEDLKAGRMVVLVDDEDRENEGDLVIAAEHCTPEA
                     INFMVTHARGVVCLALTGEQCDRLHLHPQVDVNTAQMGTAFTVTVDAHPKFSVTTGVS
                     AKDRAKTIEVAIAEDAQPQDLLRPGHINPLRARTGGVLVRAGQTEGSVDLARIAGLKP
                     AAVICEIMREDGEMARRPELETFCAKHGIRMCTIADLISFRLKREQFVKRIETITLPT
                     QWGLFTLHAYQSAIDPQPHLALCKGGIGDLNIHGVTIPHPEPVLVRVHSECLTGDIFG
                     SARCDCGPQLSTAMQMIEAEGKGVLLYLRQEGRGIGLANKLHAYALQDRGLDTVEANQ
                     RLGLPVDKRDYGIGSQILRDLGLSKLRIMTNNPKKIYGIDGYGLQVVEEVPIRIPPGE
                     HNRRYLETKKAKMGHTL"
     gene            complement(1316..1726)
                     /locus_tag="VLI90_04020"
     CDS             complement(1316..1726)
                     /locus_tag="VLI90_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13398.1"
                     /translation="MNRAVREALSAYTYDNNRWLSHANYDIPALLTHIILKARYARNL
                     LQHKRCPINRCNINDYAELTRRMLKFHISGTVIVLFISSAQPPHRKTSDEGLNNSPFF
                     PPMWRTLPSHNPKRFPTAPFEAVLARDGFFYAPK"
     gene            1796..2725
                     /locus_tag="VLI90_04025"
     CDS             1796..2725
                     /locus_tag="VLI90_04025"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSV13399.1"
                     /translation="MRILVTGGAGFIGSNLARRLFNLGHDVVAADSFGSAHFSNLTDF
                     AGDVLTLKDDEDVDSMIALGPFEAIFHQASITGVVGAHGEDQSSQHRMMRNNVETFRR
                     LLDWARQTEARVIWASSCSVYGRGAVPMIETQRPDPLNVYAFSKLTMERMAARYAPKL
                     AHPIVGLRYSNVYGPGEDHKGRLASMIHQLAKQMRAGKRPQIFTAGEQKRDFVYIDDA
                     VQANIAAITAKQAGVFNAGAGRSWSFNQVVAELNRVLKTDLPPEYFDNPYAFTQDWTE
                     TDQTLAKRVLGYEPKFDLARGIDAYHASGKLGV"
     gene            2910..>3555
                     /locus_tag="VLI90_04030"
     CDS             2910..>3555
                     /locus_tag="VLI90_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HSV13400.1"
                     /translation="MIAPTVHAEVGRAWAFRNTRFNDGWKFLKSDAVGAEATEFDDHS
                     WRDIQLPHDWSIEGPFDQKWASGTGYLPGGIGWYRKTFSLTSAQAGRRISIRFDGVYK
                     NSKVWCNGQLLGERPNGYVSFEYDLTPYLRPMGQTNVIAVRVDHSDFADSRWYPGSGI
                     NRDVWLINTGDVHVPTNGTFVTTPQVDAHRAVVDVRTSVKNDGTEPAKVTLVSTI"
BASE COUNT          700 a         1020 c         1176 g          659 t
ORIGIN      
        1 cttggcgaac ctgcgtgttt cgccgcggca tctcacctac aattgttcat tatgtcattc
       61 gcttccattg ctgattgtct cgaagacctc aaggccggcc ggatggtggt gctggtggac
      121 gatgaggatc gtgagaacga aggcgatctg gtcatcgcgg ccgagcactg tacgccggag
      181 gcgatcaatt tcatggtcac gcacgcgcgc ggcgtcgtct gcctggcgct gaccggcgag
      241 cagtgcgatc gcctgcatct gcatccgcag gtcgatgtga acaccgccca gatgggcacc
      301 gcgttcacgg tgaccgttga tgcgcatccg aagttcagcg tgacgacggg cgtgagcgcg
      361 aaggatcggg ccaagaccat cgaagtggcg atcgctgaag acgcccagcc gcaggacctg
      421 ctgcggccgg ggcacatcaa cccgctgcgc gcccgcaccg gcggcgtgct ggtgcgcgcg
      481 gggcagaccg aaggcagcgt cgacctggcg cgcatcgcgg gactgaaacc cgcggcggtc
      541 atctgcgaga tcatgcgcga agacggcgag atggcccgcc ggccggaact ggaaacgttc
      601 tgcgccaagc acggcatccg catgtgcacg atcgcggacc tgatcagctt tcgattgaag
      661 cgcgagcagt tcgtgaagcg gattgagacg atcacgctgc cgacccagtg gggcctgttc
      721 acgctgcacg cctatcagag cgcgatcgac ccgcagccgc acctggcgct gtgcaagggc
      781 ggcatcggtg acttgaacat ccacggcgtg acgatcccgc accccgagcc ggtgctcgtc
      841 cgcgtgcaca gcgaatgttt gaccggtgac atcttcggct ccgcgcgttg cgactgcggc
      901 ccgcagttgt cgacagcgat gcagatgatc gaagccgaag gcaaaggcgt gctgttgtac
      961 ttacgacagg aaggtcgcgg gatcggcttg gccaacaagc tgcacgcata cgcgctgcag
     1021 gatcgggggc tggacacggt cgaagcgaac cagcggctgg gattgccggt ggacaagcgc
     1081 gactacggta tcggcagcca gatcctgcgc gacctgggct tgagcaagct gcggatcatg
     1141 acgaacaacc cgaagaagat ttatgggatc gacgggtatg gcttgcaggt ggtggaggag
     1201 gttccgatcc ggatccctcc cggggaacac aaccggcgct atttggaaac gaagaaggcg
     1261 aagatggggc acacgctctg acggaagaaa agcccaggga tagctatccg tgggcttatt
     1321 tcggcgcata aaaaaagccg tcccgggcaa ggacggcttc gaatggggct gttggaaaac
     1381 gcttcgggtt gtgggaggga agcgttctcc acatcggggg gaagaatggg ctattgttca
     1441 agccctcgtc ggacgtttta cggtgcggcg gttgggctga tgaaatgaat agaacaatca
     1501 ctgtgccgga aatgtggaat ttcagcatgc ggcgggttag ttcggcataa tcgtttatgt
     1561 tgcagcgatt tatcggacag cgcttatgct gcaagagatt tctggcgtat ctcgctttca
     1621 aaataatgtg cgtcaaaagg gcgggaatgt cgtagttagc gtgcgacaac catcgattgt
     1681 tgtcgtacgt gtaagcgctt aaggcttcac gaacggcgcg attcaaactc gcttcgcttt
     1741 gacgtttccc gcgtctgacc ctaccttctc gcctcgttcg gaataggagg cccctttgcg
     1801 aattctggtc accggtggcg cgggcttcat cggttccaac ctcgcccgcc ggctgttcaa
     1861 cctcgggcac gatgtggtcg ccgccgactc gtttgggtcg gctcacttct ccaatctgac
     1921 tgatttcgcg ggcgacgtgc tgacgctcaa ggacgacgag gacgtcgatt cgatgatcgc
     1981 gctcgggccg ttcgaggcga tcttccacca ggccagcatc accggtgtcg tcggcgccca
     2041 cggcgaagac cagagcagcc agcatcgcat gatgcggaat aacgtcgaga cgttccgccg
     2101 attgctcgac tgggcccggc agaccgaggc ccgcgtcatc tgggcgagca gctgcagcgt
     2161 ctatgggcga ggcgcggtgc cgatgatcga gacccagcgg ccagacccgc tcaacgtcta
     2221 tgccttcagc aagctgacga tggaacgcat ggccgcgcgg tatgcgccga agctggcgca
     2281 cccgatcgtc ggcctgcgct acagcaacgt gtacggcccc ggtgaagatc acaagggccg
     2341 gctcgccagc atgatccacc aattggcgaa acagatgcgc gccggcaagc gcccgcagat
     2401 cttcaccgcg ggcgagcaga agcgcgactt cgtctacatt gacgacgcgg tgcaggcgaa
     2461 catcgccgcg atcaccgcga agcaagccgg cgtcttcaac gccggcgccg ggcggtcgtg
     2521 gtcgttcaat caagttgtcg cggagttgaa tcgagtgttg aagaccgatc tgccgccgga
     2581 gtatttcgac aacccgtatg cattcacgca ggactggacg gagacggacc agacgctggc
     2641 gaagcgcgtg ctgggttatg agccgaagtt tgacttggca cgcgggatcg atgcgtatca
     2701 cgcatcgggt aagctcggcg tttgacgagc atcaacgtga cacgggcctc cggcccgtgc
     2761 gaacggcgtc gaaccttgcg cgaactgcgg atcctcaatt ctaaaacacc tcgcgcgggc
     2821 cggaggccgg tgtctcggtg agcatctcag ggtgacgaat gacggcaacg gtggcgagat
     2881 atctggcgtg tgccttaggc gttctatgtg tgattgcgcc gacggtgcat gccgaagtgg
     2941 ggcgtgcctg ggcgtttcgc aacacgcggt tcaacgacgg ctggaagttc ctaaagtccg
     3001 acgccgtggg ggcggaggcg accgagttcg acgatcacag ctggcgcgat attcagctgc
     3061 cgcacgactg gagcatcgag gggccattcg atcagaaatg ggccagcggc accgggtatt
     3121 tgcccggcgg gattgggtgg tatcgcaaga cgttttcgct gacctccgcg caggcggggc
     3181 gtcgcatctc gatccgcttc gacggcgttt acaagaacag caaagtctgg tgcaacgggc
     3241 agctgctcgg tgagcggccg aacggttacg tcagttttga gtacgacctg acgccgtacc
     3301 tgcggccgat ggggcagacg aacgtcatcg ccgtccgcgt cgatcacagc gactttgccg
     3361 actcgcgctg gtatcccggc tcgggcatca accgcgacgt ctggttgatc aacactgggg
     3421 acgttcacgt gccgaccaac ggcacgttcg tcaccacgcc gcaggttgat gcgcatcgcg
     3481 cggtggttga tgtgcgcacg agcgtgaaga atgacggcac ggagccggcg aaggtgacgc
     3541 ttgtcagcac gatct
//
LOCUS       DATIMH010000240         1879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2361287, whole genome shotgun sequence.
ACCESSION   DATIMH010000240 DATIMH010000000
VERSION     DATIMH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361287"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1409)
                     /locus_tag="VLI90_04035"
     CDS             complement(18..1409)
                     /locus_tag="VLI90_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV13401.1"
                     /translation="MTPSTEPSPTSAPALPYAAPLQRPASSYRWVICALLFLATTINY
                     MDRQLLGLLAPILEKAIHWTETDYGHIVIAFQAAYAIGQVSCGWLIDRWGTKRGYSLS
                     IIVWSFAAAAHGLARSVLGFGVARFALGLGEAGNFPAAIKTVAEWFPKKERALATGIF
                     NSGSTVGAIIAPLIVPPLALAFGWQAAFVVLGATGLLWLILWLALYDRPDVSRRVSPS
                     ELAYIRSDAEAATTLADAVEPADAAVEAVIAEPPPVRWGQLLRHRQTWAYNIQSLCVQ
                     PIWWFYLFWLPKFFVSHFGLELQKSGQFLAVTYTMSMIGSVSAGLFSAFLLRRGWSVN
                     ASRKTALLACALLTIPMTLVAHTRSVWLATALIGLTLAGMQGWASNAYTIVSDLFPKR
                     AVASIVGLGSACGSLAAIVLAEVVGKVLQKTGSYNGLFIVAGLSLPLAVTLLHLLAPR
                     WEPVRLDGLDSSS"
     gene            complement(1415..>1879)
                     /locus_tag="VLI90_04040"
     CDS             complement(1415..>1879)
                     /locus_tag="VLI90_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HSV13402.1"
                     /translation="GSATAVFDHCEIHSRNGGFVTAANTPQDKPFGFVFMNCKLTGDA
                     IPWDPASTNPATTQKARVTPMADLGRPWRAYANVIYLNCEMGAHINPAGWNNWGKAEN
                     EKTARYAEYNSTGPGANPQKRVPWSKQLSKDEAEKITIERVLGGNDGWKPAE"
BASE COUNT          342 a          650 c          570 g          317 t
ORIGIN      
        1 acgagtacgt gctccgctca ggatgaacta tccaaaccat ccaaccgcac cggctcccac
       61 cgcggggcca gcaggtgcag cagcgtgacc gccagcggca gcgataagcc cgcaacgatg
      121 aacagcccgt tatagctgcc cgtcttctga agcaccttcc cgaccacctc cgccagtacg
      181 atggccgcga gcgagccgca cgcgctgccg aggccgacga tcgatgcgac ggcgcgcttc
      241 ggaaataaat ccgacacgat cgtgtacgcg ttcgacgccc agccttgcat ccccgcgagc
      301 gtcaggccga tcagcgctgt cgccaaccac acgcttctgg tgtgagccac cagcgtcatc
      361 ggaattgtga gcagcgcgca ggccagcagt gccgtcttcc ggctcgcgtt gactgaccag
      421 ccgcggcgca agagaaatgc cgagaacaac ccagcgctga cgctgccgat catcgacatc
      481 gtgtacgtca ccgccaaaaa ctgtcccgat ttctgcagct ccaacccgaa atggctgacg
      541 aaaaattttg gcagccaaaa caagtagaac caccagatcg gctggacgca cagtgattgg
      601 atgttgtacg cccaggtctg gcgatgccgc agcaattgcc cccagcgcac cggcggcggt
      661 tcagcaatga ccgcctcgac cgcggcgtcc gctggttcca ccgcgtcggc cagcgtcgtt
      721 gccgcttcag catccgatcg aatatatgcc agctcgctcg gcgacacgcg tcgcgacacg
      781 tcgggccggt cgtacagcgc gagccagagg atcaaccaca gcagcccggt cgcgccgagc
      841 acgacgaatg ccgcctgcca cccgaacgcc agcgccagcg gcgggacgat cagcggcgcg
      901 atgatcgcgc ccaccgtcga gccgctgttg aagatgcccg tcgccagcgc gcgttccttc
      961 ttcggaaacc actcggccac cgtcttgatg gcggcgggaa aattgccggc ctcgcccagg
     1021 cctagcgcga agcgggcgac gccgaaaccg agcaccgatc gcgccagccc gtgcgccgcg
     1081 gcagcgaagc tccagacgat gatcgacagg ctgtagccgc gcttcgtgcc ccagcggtcg
     1141 atcaaccaac cgcagctgac ctgcccgatg gcgtatgccg cctgaaatgc gatcacgatg
     1201 tggccgtaat ccgtctcggt ccagtggatc gccttctcca ggattggcgc gagcaacccg
     1261 agcagctgcc ggtccatgta gttgatcgtc gtcgccagga acagcagcgc gcagatcacc
     1321 cagcggtatg acgacgccgg ccgttgcagc ggcgccgcat atggaagcgc gggcgcggac
     1381 gtgggcgatg gttcggttga cggcgtcatg tccttcactc cgccggcttc caaccgtcgt
     1441 tgccgccgag cacgcgctcg atcgtgatct tttccgcttc gtccttgctc agctgcttcg
     1501 accacggcac gcgcttttgc ggattcgcgc ccgggccggt gctgttgtac tcggcatagc
     1561 gcgccgtctt ttcgttttca gccttgcccc agttgttcca acccgcgggg ttgatgtgcg
     1621 cgcccatctc acagttgaga tagatgacgt tcgcatacgc ccgccacggg cggccgaggt
     1681 ccgccatggg cgtcacgcgc gccttctgcg tcgtcgccgg gttggtgctg gccgggtccc
     1741 acggaatcgc gtcgccggtc agcttgcagt tcatgaagac gaagccgaat ggcttatcct
     1801 gcggcgtgtt cgccgccgtc acgaaaccgc cgttgcggct gtggatctca cagtgatcga
     1861 agaccgcggt ggccgagcc
//
LOCUS       DATIMH010000241         3330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5540029, whole genome shotgun sequence.
ACCESSION   DATIMH010000241 DATIMH010000000
VERSION     DATIMH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5540029"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="VLI90_04045"
     CDS             <1..1361
                     /locus_tag="VLI90_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSV13403.1"
                     /translation="DEPTNHLDLAAIEWLEKYLLGFSGSVVLISHDRYLLDRLATRIV
                     WLTQAKIKSYPGNYSAFLEQKELEELSQNRAYEQQQADIDKQKEFVRRFGAGQRAREA
                     KGREKRLNRLLKSDQLIGAVATQSKIRLQLDTDQRAGDQVLTVRELSKTYGTKKLWDD
                     VGFFVKRGERIGIIGPNGSGKTTMLQVLVGQADADRGTVKWGANLSLGYYDQRLDDFD
                     PEATVLEEAAADREGVTIKEVRDVLALMLFRGDDIEKPIKLLSGGERARVRIAQLLLD
                     RPNVFLMDEPTNHLDIASREALEGALKDFPGTIMCVSHDRYFLDQVASRLLVLDPPRV
                     KDFEGGYSAWATKQSAPAPKATAAPAKAKQAAAPQKPSNKPAAPKKDNPYARPFGRLS
                     VQELEKQIARTEQDLKRCQAAFGDQKIMRDGHKGRQVQAEHDELAAKLEALEAEYFAR
                     EK"
     gene            1656..2342
                     /locus_tag="VLI90_04050"
     CDS             1656..2342
                     /locus_tag="VLI90_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV13404.1"
                     /translation="MAVRLEQLKKRYVGPDGSVVPVVDIERFDLADGEQVALVGTSGS
                     GKTTLLHLIAGILAPDSGRILFGQGNGDMVDLAGLSEANRDVFRGRNIGYIFQTHHLL
                     PGFTAMENVQLGMSFTGRAHDPDWAKHLLNEVGLAERLNYKPAKLSVGQQQRVAIARA
                     LANRPKLVLADEPTGALDAVSAQQALDLIRKLCGEVSASLLLVSHDPNITRQLPRAVD
                     LKELNRAAQM"
     gene            2431..>3330
                     /locus_tag="VLI90_04055"
     CDS             2431..>3330
                     /locus_tag="VLI90_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV13405.1"
                     /translation="MRQRALGTWLTLLSVMLGVALAIAIMILRREAGTLFGQKEYGYD
                     VIVGKKGSGTQLVMNTVYHLDVSPGNISYQLYQNLLHSPQFRPLVRIAVPYVVGDTYQ
                     GKYRIVGTEASLFGYDDEGNKLDDAKVLEYRPGKRYQIVEGRDFAPNKFEAVIGSDLA
                     KLTDLKIGKTFRATHGMPRPDETPDIHPEVWTVVGILAPTHTANDRVVFLPFRSLYTI
                     GEHDLALKAQWAIQHGQPAPPSNVDPDQVKSYKLNDNGTFDLFVPQEQWEVSAILVKA
                     RGEFPAQSLIYTLSNGNEAMAVNP"
BASE COUNT          677 a          959 c         1102 g          592 t
ORIGIN      
        1 tggatgagcc gaccaatcac ctcgacctcg ccgcgatcga atggctcgaa aagtacctgc
       61 tcggcttcag cggatcggtc gtcctcatca gccacgatcg ctacttgctc gatcgcttgg
      121 cgacgcgcat cgtctggctc acgcaggcga agatcaaaag ctatcccggc aactattccg
      181 cgttcctcga gcagaaggaa ctcgaagagc tctcgcagaa ccgcgcctac gagcagcagc
      241 aggcggacat cgataagcag aaggaattcg tccggcggtt cggcgcgggg caacgggcgc
      301 gcgaggcgaa gggccgcgag aagcggttga atcgcttgct caagagcgac cagctgatcg
      361 gcgcagtggc gacgcagagc aagatccgcc tgcagctcga taccgaccag cgcgcgggcg
      421 atcaggtgtt gaccgtgcgc gaattgtcga agacatacgg cacgaaaaaa ctttgggacg
      481 acgttggctt cttcgtcaag cgcggcgagc gcatcggcat catcggaccc aacggatcgg
      541 gcaagacgac gatgctgcag gtgctggtcg gccaggccga cgccgaccgc ggcacggtca
      601 aatggggcgc gaacctgagc ctcggttatt acgaccagcg gctggacgat ttcgatcccg
      661 aagccacggt gctggaagaa gcggcggccg atcgcgaagg cgtgaccatc aaggaagtgc
      721 gcgacgtgct ggcgctgatg ctcttccgtg gcgacgatat cgagaaaccg atcaagctat
      781 tgagcggcgg cgagcgagcg cgggtgcgga tcgcgcagct gctgctggat cggccgaacg
      841 ttttcctgat ggacgagccg acgaatcacc tggacatcgc atcgcgcgag gcgttggagg
      901 gggcgctgaa agattttccg gggacgatca tgtgcgtcag ccacgatcga tattttctgg
      961 atcaggtggc gagtcggctg ctggtgctgg acccgccgcg ggtgaaggat ttcgagggcg
     1021 ggtatagcgc gtgggcgacg aagcagtctg cgccggcgcc gaaggcgact gcggcacccg
     1081 caaaggccaa gcaggcggca gcgccacaga agccctcaaa taaaccggcg gcgccgaaaa
     1141 aagataatcc gtatgcgcgg ccgttcgggc ggctcagcgt gcaggaattg gaaaagcaga
     1201 tcgcgcgaac ggagcaagat ctcaaacgct gtcaggcggc gtttggtgac cagaaaatca
     1261 tgcgcgacgg ccacaagggc cggcaggtgc aggcggagca cgatgagctg gcggcgaagc
     1321 tcgaagcgct tgaagcggaa tattttgcaa gagagaagtg atttatgttt atcgatcttc
     1381 gcgcgctggg tgctggcttt cgttctggtc ccctcgcccg gtaatccggg agagggttag
     1441 ggtgagggtg atttggttta tcgatgaacg ttcgacgttc ggaatcaccc tcacccccgc
     1501 cctctcccgg aggcctgcgg tcagcagtac ggggcgaggg ggtaagagcg gcgttgcgtt
     1561 gcaccgcggt ttcggcgcag tccgtgcaac ttccgcacgg cccgcgcatt tcgacgattg
     1621 cacgcgggaa gtgacacctc tgattttgga gcaccgtggc ggttcggctt gagcaattga
     1681 aaaagcgcta cgtcggcccc gacggcagcg ttgtgccggt ggttgatatc gagcggttcg
     1741 atctcgccga tggcgagcag gtcgcgctcg tcggcaccag cgggtccggc aagaccacgc
     1801 tgctacatct catcgccggc atcctcgcgc ccgattccgg gcgcattctt tttggccagg
     1861 gcaacggcga catggtcgat ctggccggcc tttccgaagc caatcgcgac gtcttccgcg
     1921 gtcgcaacat cgggtacatc tttcaaacgc atcacctgct gccgggcttc accgcgatgg
     1981 agaacgtgca gttggggatg agcttcaccg gccgggcgca cgatcccgac tgggcgaaac
     2041 acctgctgaa cgaagtggga ttggccgagc ggttgaacta caagcccgcg aagttgtcgg
     2101 tcgggcagca gcagcgcgtc gcgatcgcgc gagcgctggc gaatcggccc aagctggtgc
     2161 tggcggatga accgaccggc gcgctggatg cggtgagcgc gcagcaggca ttggatttga
     2221 ttcgcaagct ctgtggtgaa gtatccgcga gcttgttgct ggtctcacac gatcccaaca
     2281 ttacgcggca gctgccgcgc gcggtggacc tgaaggaact taatagagct gctcaaatgt
     2341 agcgggcgtc gcccgtgcca aggtgctctg acacgggcga gacgcccgtg ctactcaatc
     2401 atgaatctct ttcaactagt cctcaaacag atgcgccaac gcgcgctcgg cacgtggctc
     2461 acgctgcttt ccgtgatgct gggcgtggcg ctggcaatcg cgatcatgat cctgcgccgc
     2521 gaagccggca ccctctttgg ccagaaggaa tacggctacg acgtcattgt cggtaagaag
     2581 ggctctggca cccagctggt catgaacacg gtgtatcacc tggacgtcag cccggggaac
     2641 atctcgtatc agctgtatca gaacctgctg cattcgccgc agtttcggcc gctggtgcgc
     2701 atcgcggtgc cgtatgtggt gggcgatacg tatcagggca agtaccgcat cgtcggcacc
     2761 gaggcgtctc tgtttggtta cgacgatgaa gggaacaagc tggatgacgc gaaggtgctg
     2821 gagtatcggc cgggcaagcg ctatcagatc gtcgaaggcc gcgacttcgc gccgaacaag
     2881 tttgaagcgg tgatcggttc cgatctggcg aagctgaccg acctgaagat cggcaaaaca
     2941 tttcgcgcga cgcacggcat gccccggccc gatgagacgc cggacattca cccggaagtg
     3001 tggacggtcg tcggcattct cgcgccgaca cacaccgcca acgaccgcgt cgtgtttctg
     3061 ccgttcagaa gtctgtacac gatcggcgaa catgatctgg cgctcaaggc gcagtgggcg
     3121 atccagcatg gtcaaccggc gccgccgtcc aacgtcgatc cggatcaagt gaagtcgtac
     3181 aagctgaacg acaacgggac gtttgacctg ttcgtgccgc aggaacagtg ggaggtgtcg
     3241 gccatcctcg tgaaggcgcg cggcgaattc ccggcgcaga gcctgatcta cacgctgagc
     3301 aacggcaacg aagcgatggc ggtgaacccc
//
LOCUS       DATIMH010000242         1809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_342338, whole genome shotgun sequence.
ACCESSION   DATIMH010000242 DATIMH010000000
VERSION     DATIMH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342338"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VLI90_04060"
     CDS             <1..574
                     /locus_tag="VLI90_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13406.1"
                     /translation="LVGPTGVGKTTTLAKLAANLKLREKHKVGLITLDTYRIAAIDQL
                     KKYADILGSPLKVVNSGEELRDAVKGMVECDFILIDTAGRSPNDTLKINELKNLLATV
                     EPDEVHLVLSSTSSESCIELAATRFAEVRVDKVIFTKVDEAAHIGVVFNVVRKLNKNL
                     SYITTGQDVPDDIEVGRGRRLAQLILGGGL"
     gene            766..1659
                     /locus_tag="VLI90_04065"
     CDS             766..1659
                     /locus_tag="VLI90_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HSV13407.1"
                     /translation="MQSATATVGVPVQQHQAAPRRRAAVVAITSGKGGVGKSNIAVNL
                     AIKLSMAGKETVLLDADLGLANADVLCNMDLRANLSHVIGRHKNLQDVIATGPGGFGL
                     IGGASGLARMADLSDLDRQRLVDALAELEQRADVILIDTGAGISPNVLTFTRAADHVL
                     VVTTPEPTAITDAYAVVKVIARQAAADGQNLNDATGQRMSLLVNQVRTAGEARVVHER
                     IAKVARQFLNVKVFDAGYVVHDDAVSDAVRRRTPFVVGAPRSAAAQCIAHLAMRLEQG
                     VAAAPAQSGGFFERMGRWFKR"
BASE COUNT          329 a          576 c          588 g          316 t
ORIGIN      
        1 gctggtcggt cccaccggtg ttggcaagac gacaacgctt gccaagctcg ccgccaacct
       61 gaagcttcgc gagaagcaca aggtgggact gatcacgctc gacacgtacc gcattgccgc
      121 gattgaccag ctcaagaagt acgcggatat ccttggctcg ccgctgaagg ttgtgaactc
      181 cggcgaagag ctgcgtgacg ccgtcaaggg aatggtcgag tgtgacttca tcctgatcga
      241 cacggccggc cggagcccca acgacacgct gaagatcaac gagctgaaaa acctgctggc
      301 gaccgtggag cccgacgagg ttcacctggt gctttccagt acgtccagcg aatcctgcat
      361 cgagctggcg gcgacgcgct ttgcggaggt gcgggtcgac aaggtgatct tcacgaaggt
      421 ggatgaggcg gcgcacatcg gcgtcgtatt caacgtagta cggaagctca acaagaacct
      481 gtcgtacatc acgaccgggc aggacgtgcc ggacgacatc gaggtcggcc gcgggagacg
      541 gctggcgcaa ctgattttgg gcggcggcct atgacgataa gacgatgtgg cggtcgtccg
      601 ccgtttgtgg gcggtggttg ccgcgggcaa cacgtcgtct ttctcgtgtg agatgcgggc
      661 aagtggatcg gaccgcgcgg ttccgcggag tggtatccgt gttcggggaa gcttatgacg
      721 gcagcagcag cagtcattga ccaggcggag cacttgcggt cgctcatgca atcggcgacc
      781 gcgaccgtgg gcgtgccggt gcaacagcac caggctgcgc cgcgccgccg cgcggcggtg
      841 gtggcgatca ccagcggcaa gggcggcgtc ggcaagagca acatcgcggt caatctcgcc
      901 atcaagctaa gcatggccgg taaggagacg gtgctgcttg acgccgatct gggccttgcc
      961 aatgccgacg ttctctgcaa catggacttg cgcgccaacc tgtcgcacgt catcgggcgt
     1021 cataagaatc ttcaagacgt catcgcgacc ggaccgggcg ggttcggtct catcggcggc
     1081 gcctcgggcc tcgcccgcat ggcagatctg tccgaccttg accgccagcg tctcgtcgat
     1141 gccctggcgg agcttgaaca gcgggccgac gtgatcctga tcgacaccgg cgccggcatc
     1201 agccccaacg tcctgacctt cacccgcgcc gctgatcacg tgctcgtcgt caccacgcca
     1261 gagccgaccg ccatcaccga cgcctacgcc gtcgtaaaag tcatcgcccg ccaggcggcc
     1321 gccgatggac aaaacctcaa cgatgcaacc ggccagcgca tgagtctcct ggtcaaccag
     1381 gtccgcacgg cgggggaggc ccgggtggtc cacgagcgca tcgccaaggt cgcccggcag
     1441 ttcctgaacg tgaaggtttt cgacgctggc tacgtcgtcc acgatgatgc cgtctccgac
     1501 gccgtccgcc gccgaactcc gttcgtcgtt ggtgccccgc gcagcgctgc cgcccagtgc
     1561 atcgctcacc tggcgatgcg attagagcaa ggcgtcgccg ccgctccggc ccagtccggc
     1621 ggcttcttcg agcggatggg gcggtggttt aaacggtagg ggtcactggt cacttgtcac
     1681 tggtcactcg cagggcccac ccgatcttga gccggccgcg agtgacgagt gacgagtgac
     1741 cagtgacgca tttatgccta cacgcatcgc cgccgcactg tcgctcatcg tcttcgcggt
     1801 ctgcgtgat
//
LOCUS       DATIMH010000243         4500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2363666, whole genome shotgun sequence.
ACCESSION   DATIMH010000243 DATIMH010000000
VERSION     DATIMH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363666"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..771)
                     /gene="gspG"
                     /locus_tag="VLI90_04070"
     CDS             complement(361..771)
                     /gene="gspG"
                     /locus_tag="VLI90_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HSV13408.1"
                     /translation="MNRRFKKRRATGFTLIELMLVMVIIAILAAVVVPKFTGQRERAN
                     QTKAKADIATLKNALSMFEVDNGRFPTTDEGLRALVEKPQGLDTWKHAYIDSLQKDPW
                     EQDYIYRSPGNGGKDYDLISSGPDKHEGGGDDIE"
     gene            complement(818..2056)
                     /locus_tag="VLI90_04075"
     CDS             complement(818..2056)
                     /locus_tag="VLI90_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSV13409.1"
                     /translation="MSVFAYTALSKDGSKTSGTLSAETRAAAIAQMSRQGLSPVRIDE
                     ARDAAAAAKKAAAAKAGAGGPVGKVSNKAVESFTRELANLLAGGVPLSRAMSLLRREA
                     SNPAAKALWNSIYEDIVGGTALADALAKYPKNFSKVYVAMVRAGEAGGFLDVVLGQIS
                     DFRTREQDLMGKVKAAMVYPIVLVVLAVGVLIFLLSYFIPQFSGIFKQFGSNLPTLTL
                     VIIAISNVVKHYILYVLAAAIIIGVVFKRAIQTQSGRRQVELTVLSTPLLGNVIAHFA
                     LVRFTRMLGTLVGAGVSLIASLRVAREAIGNQTLADTVSTAIDEVQRGQALSKSLASS
                     PRLFPPSVIEMIAVAEETGRLDKELLRIATTYESDLDRQLRLLVAVGEPVLLFMMAAM
                     IGTIVIGMLLPILNLSDVVK"
     gene            complement(2143..3882)
                     /locus_tag="VLI90_04080"
     CDS             complement(2143..3882)
                     /locus_tag="VLI90_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSV13410.1"
                     /translation="MEHVVERLLNEGLLDADGAALVRDALAAGKPLDDALRAAKGASE
                     EKILRFLAADYDIPYVDLEKDAAKYQPTKELLAKFPARILIDHRLMPIAENGDGFTVV
                     TSKVFDHSGLDELRLATGFEFHPVLATASEVDRFTKKYLGVGADTLQEMNADGEDGDV
                     KVLDDAAHEDDLDLTNAAQDASIIRFVNQVLAEALESRATDVHVEPFEDQLRIRYRVD
                     GVLIEANIPPQVRRYHAAIVSRLKILSHLDIAEKRLPQDGRIRLRVAGRDIDLRVSVI
                     PMVHGEAVVLRILDRGDASFGLEHLGMAKRDRNAWDKVLDLPHGIILVTGPTGSGKTT
                     TLYAALTKINQTDLKIITIEDPVEYQLRGINQIQVNTKSGLTFASGLRAILRHDPDVV
                     LIGEIRDRETAEIAVQASLTGHLVFSTLHTNDAPGAATRLVDMGVEPYLVSSSLEMVV
                     AQRLVRLICPHCKEEAPEEETEKLRNEFGAEVPDVIYRGRGCRNCQNTGFRGRQGVFE
                     MMPLTDEIRALILERASSRVVRKVAVQQGMQSLREDGWRLIREGRTTPEEVLRLTKDE
                     DAIVGAEELSAVR"
     gene            complement(4023..>4500)
                     /locus_tag="VLI90_04085"
     CDS             complement(4023..>4500)
                     /locus_tag="VLI90_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13411.1"
                     /translation="GGGGGNPPADKGGGGNGRFTGGGGGGGGGPQQWRQQLQDRMKQE
                     LGVTDEEMQALQPKIDQVMTLRRDAGGFGGGRPGRNRPGGGNNGPQSDVQQKIADLQA
                     AIDNKDSKPEEIKAKLDALRAAKTKAHEDLVKAQADLRGLLTQRQEAVLVMRGLLD"
BASE COUNT          756 a         1476 c         1359 g          909 t
ORIGIN      
        1 cgtccgtccc tcggcggcgg cttgcgtgcg ggcatagttg gccaggccga tgatgtcggt
       61 ggccgtgttg ctgctgctcc gcccgagcgc gaaggcgcga aagctcggaa tgatcgccgc
      121 cgaaacgatg gcgatgatcg ccatgacgag gatcagctcg aggagcgtaa aggcgctcgt
      181 cttgcccgtt cgctcggagc cgccgattgc gggtcgagaa tgaccaatga ccaaattcca
      241 attcccaatg aagttccaat gaccaatgga ccattgggat ccggtcattg ggacttcatt
      301 gggatttggg gcttggacat tggtcattgt tcactccgac gcgcgcccgt gcttgtgagg
      361 tcactcaata tcgtccccgc cgccttcgtg tttgtcgggg ccgctgctga tcaagtcgta
      421 gtctttgccg ccgttacccg gcgagcggta gatgtaatcc tgctcccacg ggtccttctg
      481 cagcgaatcg atgtacgcgt gcttccaggt atcgagcccc tgcggcttct ccaccagcgc
      541 gcgaagccct tcatcggtgg tcgggaagcg cccgttgtcc acctcgaaca tgctaagcgc
      601 gttcttcagc gtggcgatgt cggccttggc cttggtctga ttcgcgcgct cgcgctggcc
      661 ggtgaacttc ggtacgacca ccgcagccag aatggcgatg atcaccatga ccagcatcag
      721 ctcgatcagc gtgaaacccg ttgcccgtcg ttttttgaat cttcgattca tagtttgccc
      781 tttttcgttt gcttgcccgc ccgcccgctt gcgtggttca cttgaccaca tccgagaggt
      841 tcaaaatcgg cagcagcatg ccgatcacga tggtcccgat catcgcggcc atcatgaaca
      901 gcaggaccgg ttcaccgacg gccaccagca ggcgcagctg gcggtccaga tcggattcgt
      961 acgtcgtcgc gatccgcagc agttccttgt ccagccgccc ggtttcctcc gcgacggcga
     1021 tcatctcgat caccgacggt ggaaacagcc gcggactgct ggccaggctc ttggacagcg
     1081 cctggccgcg ttgcacttcg tcgattgcgg tcgaaacggt gtcggccaaa gtctggttgc
     1141 cgatcgcctc gcgcgctacg cggagcgatg cgatcagcga cacgcccgcg cccaccagcg
     1201 tgccgagcat tcgcgtgaag cgcaccagcg cgaagtgtgc gatgacgttg ccgagcaggg
     1261 gggtcgatag cacggtcagc tccacctgcc gccggcccga ttgcgtttgg atcgcgcgct
     1321 tgaagacgac gccgatgatg atggccgccg ccagcacgta caggatgtaa tgcttgacca
     1381 cgttgctgat cgcgatgatg accagcgtca gcgtcggcag gttcgagccg aactgtttga
     1441 agatcccgct gaactgcggg atgaagtacg acagcaggaa gatcagcacg cccaccgcca
     1501 gcacgaccag cacgatcggg tagaccatgg cggctttgac tttgcccatc aggtcctgct
     1561 cgcgcgtgcg gaagtcggag atctgcccca gcacgacgtc gaggaacccg cccgcttcac
     1621 ccgcgcggac catcgcgaca tacaccttgg aaaaattctt cggatacttc gccagcgcat
     1681 cggccaacgc ggtgccgccg acgatgtctt cgtagatcga gttccacaat gccttggcgg
     1741 cggggttgga cgcttcgcgg cgcaacagcg acatcgcgcg gctcaacggc acaccgccgg
     1801 caagcaggtt cgccaattcg cgcgtgaacg attcgacggc cttgttcgag accttgccga
     1861 ccggcccgcc cgcgccggcc ttggcggccg cggccttctt cgccgccgcc gcggcatcgc
     1921 gggcttcgtc gatccgcacc ggcgacagac cctgccgcga catctgcgcg atcgccgccg
     1981 cgcgcgtctc cgccgacagc gtcccgctgg tcttgctgcc gtccttgctc agtgctgtgt
     2041 aggcgaatac agacattgtt tgtaatgcgt gatgcgtaac tcgtaatggg taattcgtaa
     2101 aaacaagcgg ctcgctgtta cgcgttacga gttacgaatt gatcaccgaa cggcgctcag
     2161 ttcctccgcg ccgacgatcg cgtcttcgtc cttcgtcagc cgcaggactt cttccggtgt
     2221 ggtgcgccct tcgcgaatta agcgccaacc gtcttcgcgc aggctttgca tgccttgctg
     2281 cacggctacc ttgcggacga cgcgcgagct ggcgcgttcc aggatcagcg cgcggatctc
     2341 atcggtgagc ggcatcattt cgaacacgcc ctggcgaccg cggaagccgg tgttctggca
     2401 gttgcggcag ccgcgcccgc ggtagatcac gtccggcact tccgcgccga actcgttgcg
     2461 cagcttctcg gtttcttcct ccggcgcttc ttccttgcaa tgcgggcaga tcaatcgcac
     2521 cagccgctgc gcgaccacca tttccagcga tgatgagacg agatatggct caacgcccat
     2581 atcgaccaga cgcgtggccg cgcccggcgc atcgttcgtg tgcagcgtgc tgaaaaccag
     2641 gtggcccgtg agcgacgcct gcaccgcgat ttccgccgtt tcgcggtcgc ggatttcacc
     2701 gatcagcacg acgtcggggt cgtgacgcag gatggcgcga aggccggatg cgaacgtcag
     2761 gccggacttt gtgttcacct ggatctggtt gatgccgcgc aactgatatt cgacggggtc
     2821 ttcgatcgtg atgatcttca ggtccgtctg gttgatcttc gtcagcgccg cgtacagggt
     2881 ggtcgtctta ccggagcccg tcggccccgt gaccaggatg atgccgtgcg gcaggtccag
     2941 caccttgtcc cacgcgttgc gatcgcgctt ggccatgccc aggtgctcga gcccgaacga
     3001 tgcgtcgccg cggtccagga tacgcagcac caccgcttcg ccgtgcacca tcgggataac
     3061 cgacacgcgc aagtcgatgt cgcgccccgc gacgcgcaat cgaattcggc cgtcctgtgg
     3121 caaacgcttt tcagcgatgt ccaggtggct gagaattttc aatcgcgaaa cgatcgccgc
     3181 gtgataacgc cgcacctgcg gcgggatgtt cgcttcgatc agcacgccat ccacgcgata
     3241 gcggatgcgc agctgatctt cgaacggctc aacgtgcacg tcggttgcgc gagactccag
     3301 cgcctcggcc agcacctggt tcacgaagcg gatgatcgac gcatcctgag ccgcgttggt
     3361 caggtccagg tcatcttcgt gggcggcgtc gtccagcacc ttcacgtcgc cgtcttcgcc
     3421 gtcggcgttc atttcctgca gcgtgtcagc gccgacgccg aggtatttct tggtgaagcg
     3481 atcgacttcg ctcgcggtcg ccagcaccgg gtgaaattcg aaacccgtcg ccaggcgaag
     3541 ctcatcgagg cccgagtgat cgaacacctt cgacgtcacc acggtgaagc cgtcgccgtt
     3601 ttcggcgatc ggcatcagcc ggtgatcgat caggatgcgg gccggaaact tggccagcag
     3661 ttctttcgtc ggttggtact tcgccgcgtc cttctccaga tcgacatagg gaatgtcata
     3721 gtcggccgcg agaaagcgca ggatcttttc ttccgatgcg cccttggccg cgcgcagcgc
     3781 atcatcgagc ggtttgcccg cggccagcgc gtcgcgcacc agcgcagcgc cgtcggcgtc
     3841 gagcaagcct tcattcagca atcgttcaac cacgtgttcc actaccgagt actccagaca
     3901 gttaacataa ccgtgacagc cgggggcgga agccccggtt cttttcgttt ctgcgaaacg
     3961 gaccgggcct gccggccccg cggctatcac ggattctttg ctacagttgc tgcttcgcct
     4021 gattagtcga gcaggccgcg catcaccagc accgcttcct ggcgctgcgt cagcaggccg
     4081 cgcaggtctg cctgggcctt gaccagatcc tcgtgggcct tggtcttggc ggcgcgaagc
     4141 gcgtcgagct tggccttaat ctcttccggc ttcgaatctt tgttgtcgat ggcggcctga
     4201 agatcggcaa tcttctgctg cacgtccgac tgcgggccgt tgttgccgcc accgggacga
     4261 ttgcgaccgg gccggccacc accgaaacca ccggcgtcgc ggcggagagt catgacttgg
     4321 tcgatcttgg gctgaagcgc ctgcatctcc tcgtcggtga cgccgagctc ctgcttcatg
     4381 cggtcctgca gctgctgccg ccactgttgc ggtccaccac caccaccacc accaccaccg
     4441 gtgaagcgac cgttgccgcc gccacccttg tcggccgggg gattaccacc ccccccgccg
//
LOCUS       DATIMH010000244         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3520488, whole genome shotgun sequence.
ACCESSION   DATIMH010000244 DATIMH010000000
VERSION     DATIMH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3520488"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..628
                     /locus_tag="VLI90_04090"
     CDS             74..628
                     /locus_tag="VLI90_04090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSV13412.1"
                     /translation="MADRPILIVSGTNRPSSNTLRIARVIQQHYQDHNLSADFFDVTD
                     LPREVFEPTVYASKPAPFVEIQNRVYHAAGLHLVLPEYNGSFPGILKYFIDLLKFPES
                     FDRKPVAFVGLANGMFGALRAVEQLQMVFSYRNAHVYPDRVFIPAVRDKLDATGKVSD
                     PAIVERLAKQAKGFAQFAGAVAAH"
     gene            794..1783
                     /locus_tag="VLI90_04095"
     CDS             794..1783
                     /locus_tag="VLI90_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13413.1"
                     /translation="MKIEPIADDYNPLGDEWPTKLYRLPDQTVLSVDLASDARLSEWC
                     YFWLTFGDGGARAAWYRVFENGKQPPEWRPLKSSVKRVLKQTAVDDDDDEPPAQRRRA
                     RAGKVGAAEKLPPGQYLLEVKIGEGDQAYELAGIEFNVKAAPAKATPQAGEHQFSLTR
                     PRCSATAVASLIKMPKFAVRGLSNVARPMRFNAGPEFEATDLYPEYLRVNGQPAARYP
                     WPAITLLTLLPYLQEKHQITLTQSEHDKIARAITEARNAPQFFLTDAHRQAFLAKLDS
                     PFPEQEMRAYFREHNDVDDPEAGKKMLDGIRALRQCLSVLDKNSVVLLRVSGH"
BASE COUNT          394 a          620 c          545 g          352 t
ORIGIN      
        1 tccaattctt ctttggaagc tcgcgccagc cgcgcgtttg accttcccac cgcaacactc
       61 aacaatgcaa ctcatggccg accgaccgat cctgatcgtc agcgggacca atcgccccag
      121 ttccaacacg ctgcgcatcg ccagggtgat ccagcagcac taccaggatc acaacctgtc
      181 cgccgacttt ttcgacgtga ccgacctgcc gcgcgaggtg ttcgaaccga cggtctacgc
      241 cagcaagccc gcgcccttcg tcgagatcca gaaccgcgtg taccacgccg ccgggctgca
      301 cctggtgctg ccggaataca acggctcgtt ccccggcatc ctcaagtact ttatcgacct
      361 gctgaagttc cccgagagtt tcgaccgcaa gcccgtcgcg ttcgttggcc tggccaacgg
      421 aatgttcggc gcgctgcggg ccgtcgaaca gctgcagatg gtcttcagct accgcaacgc
      481 ccacgtgtat cccgatcgag tcttcatccc cgccgtgcgc gataagctgg atgcgacggg
      541 aaaagtaagt gaccccgcga ttgtcgagcg cctggccaaa caagccaagg gcttcgccca
      601 attcgcgggg gcagtcgccg cgcattgaaa cccacgccgg gttcggaggt actacgacga
      661 ctcggatctg gttcctaccg atgctacagg tcggtcttgc tcttgtatgc tttgttcagc
      721 tgaggaagac aaacgataca tcattggtgg acggaccaag tgaagcggac ctttcgcctt
      781 ccaactcatc acgatgaaaa tcgaacccat tgccgacgac tacaacccac tgggcgacga
      841 atggcccacg aagctctacc gtcttcccga ccagaccgtc ctcagcgtcg atctcgccag
      901 cgacgctcgc ctcagcgaat ggtgttattt ctggttgacg tttggcgatg gcggcgcccg
      961 cgctgcatgg tatcgggtgt ttgagaacgg caagcagccg ccggaatggc ggccgctgaa
     1021 atcttccgtc aagcgggtgc tcaagcagac cgccgtggac gatgacgatg acgaaccccc
     1081 ggcccagcgg cggcgggcgc gtgcgggcaa agtcggcgcg gcggaaaaac tgccgccggg
     1141 acagtatctg ctggaagtga agatcggcga gggcgatcag gcgtatgagc tggccggcat
     1201 cgagttcaac gtcaaagccg cgcctgccaa agccacgcca caagccggcg aacatcagtt
     1261 ctccctgacc cgcccacggt gcagcgcgac cgcagtcgcg tcactcatca agatgccgaa
     1321 gttcgccgtc cgcggtttga gcaacgtcgc ccgcccgatg cgcttcaacg ccggaccgga
     1381 attcgaggcg accgatctat atcccgagta tctgcgtgtg aacggtcagc ccgcggcccg
     1441 ctacccgtgg cccgcgatca cgctgctcac gctgctgccg tacctgcagg aaaagcacca
     1501 gatcacgctg acccaatccg agcatgacaa gatagcccgg gcgatcaccg aagcgcgcaa
     1561 cgcgcctcaa ttctttctga ctgatgcgca ccggcaggcg tttctcgcaa agctggacag
     1621 cccattcccg gagcaggaga tgcgcgcgta tttcagagaa cacaacgacg tagacgaccc
     1681 ggaggcaggc aaaaaaatgc tcgacggcat acgagcgttg cgccagtgct tgagcgttct
     1741 cgataagaat tcagtggttc tgcttcgtgt cagcggtcat tgacgtggac gcagaactct
     1801 gcgttagccg gcgtggcttc gcttgctatc cgcctggcgc gcccgcccgc aaggagctga
     1861 tttatcggac cttcttttct actaccgtga aaacatggat ttcccggagg t
//
LOCUS       DATIMH010000245         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_56675, whole genome shotgun sequence.
ACCESSION   DATIMH010000245 DATIMH010000000
VERSION     DATIMH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56675"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1037
                     /locus_tag="VLI90_04100"
     CDS             231..1037
                     /locus_tag="VLI90_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13414.1"
                     /translation="MLTADADPSTSPITRPLGQYGQLEVDAFCAGCGYNLHGQDVSMD
                     ERLGFLVCRCPECGRYHPAGHGTVANSVWMSRYAAMLLVVWIGIVLATAAAATFAMGG
                     LQAAYIGTFTWYQAASGNGQGFSGQWFYTTTPPADANPMSIPGWGSATVLIVASLALG
                     FFVGTLMVAFLWHWARWRYVLGIIMPALAASFAMWIYMSQREYQYVEQWCVSRTWTMA
                     AVQAAGMMLAIRLGRPLARAIVRSIIPPKPRQLLTFLWRVDGKTMPAASA"
     gene            1066..2013
                     /locus_tag="VLI90_04105"
     CDS             1066..2013
                     /locus_tag="VLI90_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13415.1"
                     /translation="MAQSPAFTRFMDSAKPDSDRWRAGKGYDLDAVEEMTDDERAEVV
                     RFLCDRETITWREVEVLIVLDPVVAEQALDRAEGKRSSEDEVVARRSAKLKSKSNKRA
                     MASAEPTHTPAYTRFMAKMNEHIMQWPDGQTYDVSAIAEMNDVERTEIVQLLGHRGTM
                     SWREVQVLSAIDTPEARAAVDAASNDHLSPTTRLTAGAAMHAAGRLPEFELFLAKQIR
                     SLSKKSDGMDRALELARQQPTETIKQALLWASWNQTECAPPCAATLCVLSGRASGPDD
                     PALAPMLQKLGVHNSYFDRKKAFEELSKLVGMELDTSQE"
     gene            complement(2032..>4888)
                     /gene="lptD"
                     /locus_tag="VLI90_04110"
     CDS             complement(2032..>4888)
                     /gene="lptD"
                     /locus_tag="VLI90_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HSV13416.1"
                     /translation="GRTNVVELKGPVDIRLDHTRLTADNAVIWLHPLPGGLLDEQRVE
                     ISLIGNARLTQEGIVRTETNLLVTAVVTGTIRLNGDRTARDDSGGEIYQLASAVRRGQ
                     TIAPGAPAPAKESTPQRRWSNSTATRVPTSGPTTQQVQSSSFLPKRTLELHYSDSKRE
                     VTPDGKLAYVISGDFVVRSIDPKGDMIEFLADNAVIFTDLARAKDARESDGGKDSKQI
                     ADHVVSAYFEGDVRVYTTPADGAKNALSMRADRVYYEFATDRAVMTDVVFHTVDFKKQ
                     MPIYMRASKVRQLSQGEFKAENVELTTSAFATPTYSLAADKAYVRSEPSENPNDGSRV
                     TFAAENTTLRGFGVPFFWLPESGGTMTSRGTALRGASIEDSSDFGFGVRTQWGLFETL
                     GQPAPSGLDADYRIDYFGDRGPAGGLDAKYNGGFITETTKDPWNFLGELRSYFVDDHG
                     TDHFGGLRTDVPVTDAFRGRAYLEHQHFFPDDWQAQLRLGWVSDANFRSQWFFDEFQN
                     GSPINESIYLKHQKDSEVFTLLTEWQPNDVVTTSETIQEQREVEYIPEFGYHRVGDGF
                     ADDTFTFFSDNSAAGMRFSRSGATLAEQGFGPGLLPGKPSFAYTGDPGTTTWRGDFRQ
                     EIDWPINAGPFKMVPYTFARYTVYSQGVVPPAVEPQVKTIPTGPVARSSDQNRVMAGT
                     GFRISTAFWRVDDTLQSDLLDIHRIRHVIEPEINLFASAQTIDQSRLFIYDENVDAVN
                     DVQAIQVALRQRWQTKRGGPGRWRSVDFFTFNVYGNFFANQPAKQFRDPADFRGAFFA
                     TEPETSLARNSLNMDSQWRISDSMTVLADASENLDYMKFATGSIGLAVQRDERLSYFI
                     GNRYIADLNSSILTVEMNYQLTRKYGFSAVQSFDFGAGQDVYYSFSLNRKFDRFLMAI
                     SLSYDQTTDNKGISFQFVPYGLRRGFGSDRLAQHYQQ"
BASE COUNT          861 a         1510 c         1606 g          911 t
ORIGIN      
        1 cagcccggtt gatcttgaac atcacttagt ccactcccca cgccgggttc ggaggcctgt
       61 cctgagcgaa gtcgaagggt actcgcgacg acccggattg tttcgtgagt ggaagaagaa
      121 tccgggtcgt cggagtaccc ttcgacttcg ctcaggacgg gcctgcgaac ccggcgtggg
      181 gggatcagaa attcttgcca cctcgccacg ttcatcgtca caatacatcg atgctcaccg
      241 cagacgccga tcccagtacc tcgccgatca cgcgcccgct gggtcagtac ggccaattgg
      301 aagtggacgc cttctgcgcc ggctgcggtt acaacctgca cgggcaggac gtgtcgatgg
      361 atgagcggct cggcttcctc gtctgtcgat gcccggaatg cggccgctat caccccgcgg
      421 gccacggcac ggtggccaac agcgtctgga tgtcgcgcta cgcagcgatg ctgctggtcg
      481 tgtggatcgg catcgtgctc gccaccgccg cggcggccac gttcgcgatg ggcggactgc
      541 aggcggcgta catcggcacg ttcacctggt accaagcggc cagcggcaac gggcagggtt
      601 tttcgggcca gtggttctac accaccacgc cgccggccga cgccaatccg atgagcatcc
      661 ccggctgggg ctccgcgacg gttctgatcg tcgcgtcact ggcgcttggc ttcttcgtcg
      721 gcacgctgat ggtcgcgttc ctctggcatt gggcgcggtg gcgttacgtg ctgggcatca
      781 tcatgcccgc gctggcggcg tcgttcgcga tgtggatcta catgagtcag cgcgagtatc
      841 aatacgtcga gcaatggtgc gtaagccgca cgtggacgat ggccgccgtg caggcggcgg
      901 ggatgatgct ggcgatccgc ttgggccggc cgttggcgcg cgcgatcgtt cgctcgatca
      961 tcccgcctaa accgaggcag ctgctcacgt tcctctggcg cgtcgatggc aagacgatgc
     1021 ccgctgccag cgcctgagcc tgctgccgcc gtagaatgcg ccgccatggc acaatccccc
     1081 gccttcacgc ggttcatgga cagcgccaag cccgactccg accgctggcg cgcgggcaaa
     1141 ggttacgatc tggacgccgt cgaggagatg accgacgacg agcgggccga ggttgtgcgc
     1201 ttcctgtgcg atcgcgaaac gatcacctgg cgcgaagtcg aagtgctgat cgtgctcgac
     1261 ccggtcgtgg ccgagcaggc gctggatcgc gccgagggaa agcgatcgtc ggaagacgag
     1321 gtggttgcgc ggcgatcagc gaagctgaaa tcgaagtcga acaagcgcgc gatggcctcc
     1381 gccgagccga cgcacacccc cgcgtacacg cgcttcatgg cgaagatgaa cgagcacatc
     1441 atgcaatggc cggacggcca gacatacgac gtgtccgcca tcgcggagat gaacgatgtc
     1501 gagcgcactg agatcgtcca gttgctgggg catcgcggga cgatgagctg gcgagaagtg
     1561 caggtgctgt cggcgatcga cacgcccgaa gcgcgtgccg cggtggacgc ggcgtctaac
     1621 gatcacttgt caccaacgac gcgcctgacc gccggcgccg ccatgcacgc ggcggggcgg
     1681 ttgccggagt tcgagctgtt tctggcgaag cagattcgca gcctgtctaa aaagtcggac
     1741 ggaatggatc gcgcgctgga actggcgcgg cagcagccaa ccgagacgat caaacaggcg
     1801 ttgctgtggg caagctggaa tcaaaccgaa tgcgcgccac cgtgcgccgc cacgctttgc
     1861 gttttatcgg ggcgggcgag tgggccggat gacccggcgt tagcgccgat gctccaaaag
     1921 ctgggcgtgc ataacagtta tttcgatcgc aaaaaagcat tcgaggagtt gtcgaagctc
     1981 gtcgggatgg agctggatac gagccaggag taggcggtcg aatcgacgcg ttcactgctg
     2041 ataatgctgc gccaatcgat ccgatccgaa cccgcgccgt agcccgtacg ggacgaactg
     2101 gaacgagatc cccttgttgt cggtggtctg gtcgtacgac aggctgatcg ccatcaggaa
     2161 gcggtcgaac ttccggttga gggaaaacga gtagtacacg tcctgtccgg cgccgaagtc
     2221 aaagctttgc accgcgctga agccgtactt gcgggtcaac tggtagttca tctcgacggt
     2281 taagatgctc gaattgagat cggcgatgta tcggttgccg atgaagtaag acagccgctc
     2341 gtcgcgttgc acggccagcc cgatcgatcc cgtcgcgaac ttcatgtagt ccaggttctc
     2401 cgatgcatcc gccagcaccg tcatcgaatc gctgatgcgc cactgcgaat ccatgttcag
     2461 gctgttgcgc gccaggctgg tctccggctc agtcgcgaag aacgcgcccc ggaaatcggc
     2521 ggggtcgcga aactgcttgg cgggctggtt ggcgaagaag ttgccgtaga cgttgaacgt
     2581 gaagaagtcc acgctgcgcc agcgccccgg gccgccgcgt ttggtctgcc agcgctgccg
     2641 cagcgcgacc tggatggcct gcacgtcgtt gaccgcgtcc acgttctcgt cgtagatgaa
     2701 cagccggctt tggtcgatcg tctgagcgct ggcgaagaga tttatttctg gctcgatgac
     2761 gtggcggatg cggtggatgt ccagcaggtc cgactgaagc gtgtcatcga ctcgccaaaa
     2821 cgcggtgctg atgcgaaagc ccgtgcccgc catcacgcga ttttgatcgc tcgatcgtgc
     2881 gaccggcccg gttgggattg ttttcacctg cggttccacc gccgggggga cgacgccttg
     2941 cgaatagacc gtgtaccgcg cgaacgtgta cgggaccatc ttgaacggac cggcgttgat
     3001 gggccaatcg atctcctgcc gaaagtcgcc gcgccacgtg gtcgtgccgg gatcacccgt
     3061 gtaggcgaac gaaggttttc ccggcaaaag ccccgggcca aaaccctgct cggccagcgt
     3121 ggcgccgctc ctggaaaagc gcatccccgc cgcgctgttg tcgctgaaga acgtgaacgt
     3181 gtcgtcggca aagccatcgc cgacgcggtg gtaaccgaac tccggaatgt attcgacctc
     3241 gcgctgttcc tggatcgtct ccgacgtcgt caccacgtcg ttcggctgcc actcggtcag
     3301 cagcgtgaag acctccgaat ccttctggtg cttcaggtag atcgattcat tgatcggcga
     3361 gccgttctgg aactcgtcga agaaccattg cgatcggaag ttggcatcgg agacccagcc
     3421 cagccgaagc tgcgcctgcc agtcatcggg aaagaagtgc tgatgttcca gataagcgcg
     3481 gccgcgaaac gcatcggtca cggggacatc cgtgcgcagt ccgccaaaat gatcggtgcc
     3541 gtggtcgtca acgaaatagc tccgcagctc gcccaggaaa ttccacgggt ccttggtggt
     3601 ctcggtgatg aacccgccgt tgtacttggc atcgagccca ccggcggggc cgcgatcacc
     3661 gaagtagtcg atgcgatagt cggcatcgag cccgctgggc gcgggttggc ccagcgtctc
     3721 gaacaatccc cactgcgttc gcacgccgaa gccaaagtcg ctgctgtctt cgatcgacgc
     3781 gccgcgcaat gctgtcccgc ggctggtcat cgtgccgccg gattcgggca gccagaagaa
     3841 cggcacgccg aaaccccgca gcgttgtgtt ctctgcggcg aacgtcacgc gcgaaccgtc
     3901 gttgggattt tccgacggct cggatcgcac atacgccttg tccgccgcca ggctgtaggt
     3961 cggcgtcgcg aaggcgctgg tggtcagttc gacgttctcg gccttgaact cgccctgcga
     4021 cagctgccgg actttgctgg cccgcatgta gatcggcatc tgctttttga aatcgaccgt
     4081 gtggaacacg acgtcggtca tgaccgcgcg gtcggtggcg aactcgtaat acacgcggtc
     4141 ggcgcgcatc gacagcgcgt tcttcgcccc gtcggccggc gtggtgtaga cgcgcacgtc
     4201 gccttcgaaa taggccgaga cgacgtgatc cgcgatctgc ttcgagtcct tgccgccgtc
     4261 gctttcccgg gcgtccttcg cgcgcgccag atcggtgaag atgacggcgt tgtcggcaag
     4321 gaactcgatc atgtccccct tgggatcgat cgaccgcacg acgaagtcgc cgctgatgac
     4381 gtacgccagc ttcccatcgg gggtcacttc gcgcttggag tcgctgtagt gaagctccaa
     4441 cgttcgcttg ggcaggaacg acgaggactg cacttgctgg gtggttggcc cgctggtggg
     4501 cacgcgggtg gcggtcgaat tgctccaccg ccgctgcggg gttgactcct tcgcaggggc
     4561 cggcgcgccc ggcgcaatcg tctgcccccg gcgaacggct gacgccaact ggtagatctc
     4621 gccgccgctg tcatcgcgcg cggtgcggtc gccgttcagt cggatcgtcc cggtgacgac
     4681 ggcggtgacc agcaggttcg tctcagtgcg aacgatgcct tcctgcgtca ggcgggcgtt
     4741 gccgataaga gagatttcca cgcgctgctc gtcgagcaac ccgccgggca gcgggtgtag
     4801 ccagatgacg gcgttgtcgg cggtgaggcg ggtgtggtcg agccggatat caaccggccc
     4861 cttgagctcg acgacgttgg tgcgcccc
//
LOCUS       DATIMH010000246         7965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3809226, whole genome shotgun sequence.
ACCESSION   DATIMH010000246 DATIMH010000000
VERSION     DATIMH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3809226"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..2726)
                     /locus_tag="VLI90_04115"
     CDS             complement(42..2726)
                     /locus_tag="VLI90_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HSV13417.1"
                     /translation="MRRIWPAVLCIAIASHIAAAAAAAAPKVALDPDAAPRVQYGAAR
                     LTSAMNGVAAAADRSIVVGTINSPSIQQLIRSDALKLAPNDLKSEGFCLSTLPDGRIV
                     VAGDNDSGALYGCLELAQRLRQGMNLPADLHFIDAPVFKLRGPCIGMQKTYILPGRHV
                     YEYPYTPELFPFFYDKQFWTEYLDFLATNRMNTLYLWSGHPFASLVKLADYPYAMEVP
                     EDVFQKNVEMYRWITAEADRRGIWVVQMFYNIILSKPFAEHNGLATQLAASTPIVDDY
                     MRKSIAEFVRQYPHVGLMLCLGEALQGIDNQKYFLTKVILPGVHDGMKQAGLTQEPPV
                     VIRTHATDLRLFMADALRIYKNLYTEAKFNGESLTTWEPRGTRQQVHLAMSQLGSTHL
                     INVHILANLEPFRYGDVNFIKRCVQAGRDRLGARGLHLYPLSYWNWPDSPDKVDPPLK
                     QWERDWIWFEAWARYSWNPDIDDATDHAYWISRLSDMYGTKESAEHILAAYNESGECA
                     PRILRRFGITEGNRQTLSLGMTLSELVHPEKFHEFPELWESQSPPGERLKEYVDREMQ
                     HQAHEGETPPQIVSEILKFSGDALAEINAAAPTRNEAEFGRLKDDINCIRLMSQFYAA
                     KVNAAMAMLRYERGKNIGDLRACVSSLSESVDFYRQLSELTERTYRFANSMQTSQRRI
                     PVPGGVDGKPANYHWTQVLPVYERELDEIKRQLSEAQSGQATPTDDAKIQPLPKATIT
                     LLDLRAQTYQVNIGAKVFTDRDDVIQSLAPELNGLTGIRLSYDQMANGLTPPIEFEVA
                     EPVTILVGYIQSTQPTWLQPPNLETDATAIDRGDVEPLIQNAATIQHLPAINIYGMKF
                     AAGKHKLEMHGRGSFIVLGVIRQSANVIKRDAHRPGGS"
     gene            complement(2782..4335)
                     /locus_tag="VLI90_04120"
     CDS             complement(2782..4335)
                     /locus_tag="VLI90_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5605 domain-containing protein"
                     /protein_id="HSV13418.1"
                     /translation="MAVKQWDVFEIALQGPSAGNPFCDVQFSAQFTWSGQPGVNVNGF
                     YDGNGRYLARFMPSVQGRWEYQTRSNAAELNGKTGSFDCIAPADNVHGPVRVHNTFHF
                     AYADGTPYRAIGTTCYAWTHQGSELEHQTLETLKASPFNKLRMCVFPKRYAYNENEPH
                     LYPFEGSPPRQWDFTRFNVDFFRYLEKRVGQLRDLNIEADIILFHPYDEGHWGFDRMD
                     ADSDDRYIRYVVARLSAYRNVWWSMANEFDFMEKKRPADWDRYFRIVQESDPHGHMRS
                     IHNGKLIYDHNKPWVSHASIQNGSAVEDFGRAVLYRDVYGKPIVFDEVKYEGNLPQRW
                     GDISAEEMVHRFWQGTIAGTYVGHGETYLHDKDVIWWSRGGTLHGKSPKRIAFLKEIL
                     ETSPPEGLEPIDKWQDVNTAGKAGEYYLVYFGRQQPSQWLFELPRIGLTPGMMFAVEI
                     LDTWNMTVTPVDGTFKIIADTTYRYHAEGQKKVKLPGHAYIALRIRRVGDTAAEKTAT
                     AESPRIYGE"
     gene            4602..6059
                     /locus_tag="VLI90_04125"
     CDS             4602..6059
                     /locus_tag="VLI90_04125"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HSV13419.1"
                     /translation="MTWLLLIASCAIGAIARADVRVAGVFADHMVLQRRVPLPLWGWA
                     EAGEHIEVKLASSAASATTDQDGKWHVELPPLEAISDGVDLTIQGKTTVAIHDVLVGE
                     VWLASGQSNMQYAVKNALNAAAALRDANRPMLRLCTVGSKASLTPLDDRGVRWSPSTS
                     DSAKGFSAVAYFFASELQQKLSVPVGIIGAYVAGTPAQSWTDLAELQSSPQLAHYVDA
                     LNRSQKAATTNPSKESAGVPTSLYNGMIAPLKSFPIKGVIWYQGESNTGEAMEYRTLF
                     PAMVRGWRKQWGDDQLPFLFVQLPGFGRRQTELAPSNWAMLREAQAMTLQLPRTGMAV
                     TVDLVPARALLHPKNKADIGHRLALLARSIAYGEAVDAWSPMFASMQIESGAARIRFT
                     HADGELVVRAAPEERAEPSTTAPSEVTGFAIAGADHKFVRATARIDADTVIVSSDEVN
                     SPVAVRYAWEENPQVDLYNAAGLPVAPFRTDDWPQ"
     gene            6094..7182
                     /gene="pelA"
                     /locus_tag="VLI90_04130"
     CDS             6094..7182
                     /gene="pelA"
                     /locus_tag="VLI90_04130"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HSV13420.1"
                     /translation="MKMKWSSLWCIAGIIILNCAVAQAVYTSAKAYLDRPAEWFKSDE
                     AAKIASNILSFQASNGGFPKNVDTASRPYTGDTATIHATFDNSATTDELRYLAHIYNA
                     TQNPRYQQAFLKGLDYVLAAQYPNGGWPQSYPPGETDYDQYITFNDGAMARLMFFIRE
                     VANDPTYNFVDAERRDHCRTAWDRGIDCILKCQVRVNGKLTAWPQQSDEKDFTPRPAR
                     KFEPIALTPNESVGICHVLMNVDHPSPAVIQAVDAAVAWMDAVKIPGIRVEDRPQKDT
                     PRGFERFVVKDPSAPPMWARYYEIGTNRPIYCGRDSVVRYDLSEIEIERRTGYQWLKY
                     WPKNLIATEYPAWKAKVEGAAASTQPSK"
     gene            7269..>7965
                     /locus_tag="VLI90_04135"
     CDS             7269..>7965
                     /locus_tag="VLI90_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13421.1"
                     /translation="MLTHLTRQVLRLEQGWRFHLGDIESPVPNTHIAAYMANKAGWSR
                     GAARVNYDDSDWRVLDLPHDWSIEGEFNPENHVDSGYLPRGIGWYRRHFRLDDADRDQ
                     HLSVRFDGIATHCTIYFNGHLLHRHFNGYTPITIDISDVATFGDQLNVIAFRVDATFM
                     EGWWYEGAGIYRHAWLIKTGKVHVAPFGVFICPRKADGDNWHTHIEATLANEMYEDRQ
                     CNVDCELFDTSGQS"
BASE COUNT         1492 a         2495 c         2440 g         1538 t
ORIGIN      
        1 tggcggaaat cttcggggcg tagcagctgc atggttcgga ttcaactccc gccggggcga
       61 tgggcgtcgc gcttgatgac gttggccgac tgccggatca cgccgagcac gatgaagctc
      121 ccgcggccgt gcatctcgag cttgtgcttg cccgcggcga acttcatccc ataaatgttg
      181 atcgccggca gatgttgaat ggtcgcggcg ttctggatga gcggctcgac atcgccgcga
      241 tcgatcgccg tcgcgtccgt ttccaggttc ggcggctgca gccacgtcgg ctgcgtcgat
      301 tggatgtacc caaccaaaat cgtcacgggc tcggccactt cgaattcgat cggtggagtc
      361 aaaccgttcg ccatttgatc gtacgacagg cgaatgcccg tcagcccgtt caactccggc
      421 gccagcgatt gaatcacgtc gtcccggtcc gtgaacacct tcgcgccaat attcacctga
      481 tacgtctgtg cccgcagatc gagcagcgtg atcgtcgcct tcggcagcgg ttggatcttc
      541 gcgtcatccg ttggagtcgc ctgacccgat tgcgcctcgc tcagctgccg cttgatctca
      601 tccaattcgc gttcgtaaac cggcagcacc tgcgtccagt gataattcgc cggctttcca
      661 tcaaccccgc ccggcactgg aatccgccgc tgcgatgtct gcatcgagtt ggcgaagcga
      721 tacgtccgct cggtaagctc agacagttgg cgataaaaat ccacgctctc gctcaagctc
      781 gacacgcacg cccgcaggtc gccgatattc ttgccgcgct cgtagcgcag catcgccatg
      841 gccgcgttca ccttggcggc ataaaactgc gacatcagcc ggatgcagtt gatgtcatcc
      901 ttcagccgcc caaactccgc ctcgttccgc gtcggcgccg ccgcgttgat ctcggccagc
      961 gcatctccgg agaacttcaa aatctcgctg acgatctgcg gcggcgtttc gccttcgtgc
     1021 gcctgatgct gcatctcgcg atcgacgtat tccttcagcc gttcccccgg cggcgattgc
     1081 gattcccaca gctcggggaa ctcatggaat ttctccgggt gcaccaactc gctcagcgtc
     1141 atgcccagcg acaacgtctg gcggttgcct tcggtgatgc cgaatcggcg caggatgcgc
     1201 ggcgcgcact cgcccgattc attgtacgcc gccaggatgt gctccgccga ttccttcgtg
     1261 ccgtacatgt cgctcagccg tgagatccaa tacgcgtgat cggtcgcgtc gtcgatgtcg
     1321 gggttccagg aatatcgcgc ccacgcttcg aaccagatcc agtcgcgctc ccactgcttc
     1381 agcggcgggt caaccttgtc gggtgaatcc ggccaattcc aataggacag cggatacaag
     1441 tgcaagccac gggcgccgag gcgatcgcgc ccggcctgca cgcagcgctt gatgaagttt
     1501 acgtcgccgt atcggaacgg ctcgaggttc gccaggatgt gcacgttgat caggtgcgta
     1561 gaaccgagtt ggctcatcgc caggtgcacc tgctgtcgcg tgccgcgcgg ttcccatgtt
     1621 gtcagcgatt caccgttgaa cttcgcctcg gtgtacaggt tcttgtagat gcgcagcgcg
     1681 tcggccatga acagccgcag gtcggtggcg tgcgtgcgga tgacgaccgg gggttcctgc
     1741 gtcaaccccg cttgcttcat cccatcatgt acgccgggca gaattacttt ggtaagaaaa
     1801 tatttctggt tgtcgatgcc ctgcagcgct tcgccgaggc agagcatcag accgacgtgc
     1861 ggatattgcc gcacgaattc ggcgatgctc ttgcgcatgt agtcgtcaac gatcggcgtg
     1921 gacgccgcca gctgcgtcgc cagcccgtta tgctccgcga agggtttcga caggatgatg
     1981 ttgtaaaaca tctgcaccac ccagatcccc cggcggtcgg cctcggcggt gatccagcgg
     2041 tacatctcca cgttcttctg aaacacgtcc tccggcacct ccatggcgta ggggtaatcc
     2101 gctagcttca ccagcgacgc gaacggatgg ccggaccaga ggtacagcgt gttcatccgg
     2161 ttcgtcgcga ggaaatcgag atactcggtc cagaattgct tgtcgtaaaa gaatgggaac
     2221 agctcgggcg tgtaagggta ttcgtacacg tgccggccgg gcaggatgta cgtcttctgc
     2281 atgccgatgc acggcccgcg cagcttgaac accggagcgt cgatgaaatg caggtcggcg
     2341 ggcaggttca tgccttgccg gaggcgctgg gcgagttcga gacaaccgta cagcgcgccc
     2401 gaatcgttat cgccggcgac aacgatgcga ccatcgggca gcgtcgatag acagaagcct
     2461 tcgctcttca aatcattcgg cgcgagcttg agcgcatcgg acctgatgag ctgctgaatg
     2521 gaaggcgagt tgatcgtgcc gacgacgata gaccgatccg ccgccgcggc aacgccgttc
     2581 atcgcgctgg tgagtcgagc cgcgccatac tgcacgcggg gggcagcgtc aggatcgagc
     2641 gcgactttgg gcgccgccgc cgccgccgcc gccgcgatgt gcgaagcgat ggcgatgcac
     2701 agcaccgcgg gccaaattcg acgcatccat tgcattcgaa atcctcgccc gcgcgctgca
     2761 catgcgtagc atggcacagc atcactcgcc gtaaatgcgc gggctctccg ccgtcgccgt
     2821 cttctccgcc gccgtgtcac ccacccggcg aatccgcagc gcgatatacg cgtgccccgg
     2881 cagcttgacc ttcttctggc cttcggcgtg atagcgatac gttgtgtcgg cgatgatctt
     2941 gaacgtgccg tccaccggcg tcaccgtcat gttccacgtg tccaggatct ccaccgcgaa
     3001 catcatcccc ggcgtcagcc cgatgcgcgg cagctcgaac aaccactggc tcggttgctg
     3061 ccggccgaag taaaccaggt aatactcgcc cgccttcccc gcggtgttca cgtcctgcca
     3121 cttgtcgatc ggctccaatc cttccggtgg cgacgtctcg agaatctcct tgagaaatgc
     3181 gatgcgcttg gggctcttgc cgtgcagcgt gccgccgcgc gaccaccaaa ttacgtcctt
     3241 gtcgtgcagg tacgtctcgc catgaccgac gtatgtgccg gcgatcgtgc cctgccagaa
     3301 gcggtggacc atctcctccg ccgagatatc gccccagcgt tgcggcaggt tgccttcgta
     3361 cttcacttcg tcgaacacga tcggtttgcc atacacgtcg cggtacagca ctgcgcgacc
     3421 gaaatcttcg acagccgatc cattctgaat gctggcgtgg ctgacccagg gtttgttgtg
     3481 gtcgtaaatc agcttgccgt tgtggatcga gcgcatgtgc ccgtgcgggt ccgactcctg
     3541 cacgattcgg aaatagcgat cccaatcagc cggacgcttc ttctccatga agtcgaactc
     3601 gttcgccatc gaccaccaga cgttgcgata cgccgacagg cgcgcaacga catagcggat
     3661 gtatcggtcg tcgctgtcgg catccatccg atcgaagccc cagtggcctt cgtcgtacgg
     3721 gtgaaacagg atgatgtcgg cttcgatgtt caagtcgcgc agctgcccga cgcgcttttc
     3781 cagatagcga aaaaaatcaa cgttgaaccg cgtgaagtcc cattgccgcg ggggcgagcc
     3841 ttcgaacgga tacaggtgcg gctcgttctc gttgtacgcg tagcgcttgg ggaacacgca
     3901 catccgcagc ttgttgaacg gcgacgcctt cagcgtctcc agggtctgat gttccaactc
     3961 actgccctgg tgcgtccatg catagcacgt cgtgccgatc gcgcggtaag gcgtgccgtc
     4021 ggcgtacgcg aaatgaaacg tgttgtgcac gcgcacgggc ccgtgaacgt tgtcggccgg
     4081 cgcgatgcag tcgaagctgc cggttttgcc gttcaactcc gcggcgttgc tgcgcgtctg
     4141 gtattcccag cggccctgaa cgctcggcat gaagcgcgcg aggtagcgcc cgttgccgtc
     4201 gtaaaacccg ttgacgttga cgcccggctg accgctccag gtgaactgcg ccgaaaactg
     4261 cacatcgcaa aacggattgc cggcggacgg tccctgcagg gcgatttcaa acacatccca
     4321 ttgtttaact gccacgcgag ttcctcaggc atggggcacg cgcactacca caattgatgc
     4381 ggcgcgcgcg tgctccgtgt tgcaatcgcc ggcggccttg gcatcggctg ccccgagaat
     4441 accactggcg attgatgggg tcgtatgacg cgcagtatga attattgtcc aaactcttgc
     4501 agaaatcgac ccggtcatgc gcgcgtcgct catggcggtg ttattacgtc atcagcgcac
     4561 gcaaaagaga atggccgccg atgcgaaatg tcagacattc tttgacctgg ctgctgctga
     4621 tcgcctcatg cgcgatcggc gcgattgccc gcgccgacgt gcgcgtcgcc ggggtgttcg
     4681 ccgatcacat ggtcctgcag cgccgcgtgc cgcttccgct atggggatgg gctgaagcgg
     4741 gcgagcatat tgaagtgaag ctggcgtcta gcgcggccag cgcgaccacc gatcaagacg
     4801 gcaagtggca cgtcgagctg ccgccgcttg aagcgatcag cgacggcgtc gatctgacga
     4861 tccagggcaa gacgacggtc gcgattcacg atgtgctcgt cggagaagtt tggctcgctt
     4921 cgggccagtc gaacatgcag tacgccgtga agaacgcgct gaacgcggcc gcggcgctgc
     4981 gcgatgcgaa tcggccgatg cttcgcctgt gcaccgtcgg cagcaaggcg agcctgacgc
     5041 cgctggacga ccgcggcgtg cgctggtcgc ccagcaccag cgattcggcc aaaggttttt
     5101 ccgccgtcgc gtatttcttc gccagcgagc ttcagcaaaa gctcagcgtg ccggtcggca
     5161 tcatcggcgc gtacgtcgcg ggcacgccgg cgcagtcgtg gacggatttg gctgagcttc
     5221 agtcgtcgcc gcagctggcg cattatgttg acgcactgaa ccggtcgcaa aaggcggcga
     5281 cgacgaatcc gtcgaaggaa agcgccggcg tgccgacgtc gctctacaac ggcatgatcg
     5341 cgccgctcaa gtcgttcccg atcaagggtg tcatttggta ccagggcgaa tcgaacacgg
     5401 gcgaagcgat ggaatatcgc acgctgttcc ccgcgatggt ccgtggctgg cgcaagcagt
     5461 ggggcgatga ccagcttccg ttcctattcg tgcagttgcc cggcttcggc cggcgacaaa
     5521 ccgagttagc gccgagcaat tgggcgatgc tgcgcgaagc gcaggcgatg acgctgcagt
     5581 tgccgcgcac cgggatggcc gtcaccgtgg acctggtgcc cgcgcgggcg ctgctgcatc
     5641 cgaagaacaa ggcggacatc ggccatcgac tggcgctgct ggcgcggtcg atcgcatatg
     5701 gcgaggcagt cgatgcgtgg tcgccgatgt tcgcgtcgat gcagatcgag tccggtgccg
     5761 cgcgcattcg attcacgcat gccgatggcg aattggttgt ccgtgccgcg ccggaagaac
     5821 gcgcggagcc atcaaccacc gcgccgtcgg aagtgacagg cttcgccatc gccggggcgg
     5881 atcacaaatt cgttcgcgcg acggcgcgca tcgatgccga caccgtcatc gtctccagcg
     5941 acgaggtgaa ttcacccgtg gccgttcgat atgcttggga ggagaacccg caggtggatc
     6001 tgtacaacgc cgccgggctg ccggtggccc cgtttcgcac ggacgattgg ccgcagtagc
     6061 tcgcgcgcaa tcccgttacg tcaggagaat ccgatgaaaa tgaaatggtc gtcgctttgg
     6121 tgcatcgcgg gaatcatcat cctgaactgc gccgtcgcgc aggcggtcta cacatccgcc
     6181 aaggcatatc tcgatcgacc cgccgaatgg ttcaagtccg acgaggcggc gaagatcgcg
     6241 agcaacattc tctcgtttca agcgagcaac ggcgggttcc ccaagaacgt ggacacggcc
     6301 agccggccgt acaccggtga cactgcgacc atccacgcga cgtttgacaa cagcgcgacg
     6361 acggacgagc tgcgttacct cgcccacatc tacaacgcga cgcagaaccc gcgttatcag
     6421 caggcgtttc tgaaggggct cgactacgtg ctggccgccc agtaccccaa tggcggttgg
     6481 ccgcaatcgt acccgccggg ggagacggat tacgatcaat acatcacgtt caacgacggc
     6541 gcgatggcgc ggttgatgtt cttcattcgg gaagtcgcca atgatccgac gtacaacttt
     6601 gtggacgccg aacgccgcga ccattgccgc acggcgtggg accgcgggat cgactgcatc
     6661 ttgaagtgcc aggtgcgcgt gaacggtaag ctgaccgcct ggccgcagca atctgacgag
     6721 aaggatttca cgccccggcc ggcgcgcaag ttcgagccca tcgcgctgac gccgaacgag
     6781 tccgtcggca tttgtcacgt gctgatgaac gtcgatcatc caagtccggc cgtgatccag
     6841 gcggtggacg ccgcggtggc gtggatggat gcggtgaaga tcccgggcat tcgagtcgag
     6901 gatcgcccgc aaaaggacac gccgcgcgga ttcgagcggt ttgtggtgaa ggatccctcg
     6961 gccccgccga tgtgggcgcg ctactacgag atcgggacga atcgcccgat ctactgcggc
     7021 cgtgatagcg tggtgcgata cgatctgtcg gagattgaga tcgagcgaag gacggggtat
     7081 cagtggctga agtattggcc gaaaaatcta atcgcgacgg aatacccggc gtggaaagcc
     7141 aaagtggaag gcgccgccgc gagcacgcag ccgtcgaagt aatcggaact tcctcgatga
     7201 gcgtccgcgt aaatgttgcc gctaaatgag acggccgtca gacggcatcg agagatttca
     7261 ccaccgccat gctcacccac ctcactcgcc aagtgctacg gttggagcag ggctggcgtt
     7321 tccacctcgg cgacatcgaa tcgcccgtgc ccaacacgca catcgccgcg tacatggcga
     7381 acaaggccgg ctggtcgcgc ggcgccgccc gcgtgaatta cgacgattcc gattggcgcg
     7441 tgctggacct gccgcacgat tggtcgatcg aaggcgagtt caatcccgag aaccacgtgg
     7501 acagcggcta cttgccgcgc gggatcggat ggtatcgccg gcactttcgc ctcgacgatg
     7561 ccgatcgaga tcagcacttg tccgtgcggt tcgacggcat tgccacgcac tgcacgatct
     7621 acttcaatgg ccacctgctg caccgccact tcaacggcta cacgccgatc accatcgaca
     7681 tcagcgacgt cgcgacgttc ggcgatcaat taaatgtcat cgccttccgc gttgatgcga
     7741 cgttcatgga aggctggtgg tacgaaggcg ccggcatcta ccgccacgcg tggctgatca
     7801 aaaccggcaa ggtccacgtc gcgcccttcg gcgtgttcat ctgcccccgc aaggccgacg
     7861 gcgataactg gcatacgcac atcgaggcaa cgctggcgaa cgagatgtat gaagatcgcc
     7921 aatgcaatgt cgactgcgag ctcttcgata catcgggtca atccg
//
LOCUS       DATIMH010000247         4479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5255117, whole genome shotgun sequence.
ACCESSION   DATIMH010000247 DATIMH010000000
VERSION     DATIMH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5255117"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..175
                     /locus_tag="VLI90_04140"
     CDS             8..175
                     /locus_tag="VLI90_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HSV13422.1"
                     /translation="MSTTNRNFPGRMGSTKSAVYLASPLTVAASALTGYVTDPREYVS
                     TKIETGMAGVL"
     gene            202..879
                     /locus_tag="VLI90_04145"
     CDS             202..879
                     /locus_tag="VLI90_04145"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HSV13423.1"
                     /translation="MGSTFRETWASLREGLHRFAAQDRQRRRQLLFRGQSDTNWPLDT
                     TLDRFKPIADPLKRARLVYELLQEFSREAVQITSRAAPLPEGDALELLARHHGLPSPL
                     LDWTRSPFVAAYFAFEAAGAADRAVWVLDLNRIPPPEFFPVDIIDDHLLLQFNRRALR
                     QQSIFLRVNEAKPLEQIASIALTKFELPSSERGIALAELDAMNINATSLFEDFDGVAR
                     AVRTRMS"
     gene            complement(1217..2065)
                     /locus_tag="VLI90_04150"
     CDS             complement(1217..2065)
                     /locus_tag="VLI90_04150"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSV13424.1"
                     /translation="MPLMQEDIRAHYEQAWKQSDDAAANDDSGLTYSNPVEDAVLYPI
                     YEQLLRDLKVNVDGGKVLDVGAGSGRWVRYFIQRFKPAMLMGVDFTQSSIDLLKRRYA
                     NVPSVTTKFRTADLTEPALDLGEKFDLINVMNVLFHIPEPDRFMTAMRNLANHLAPGG
                     RIVTTEYMPRTTMRTNWMLVRSRYDFEASAAKAGLRIVATRASCVFSNDPMGLDGPDA
                     GVRRQFNAVRTGMNQILSLQMNEETRQFFVKFLSDVESATLNFCRERMAEVDMPSQKL
                     VVLAAG"
     gene            complement(2208..3236)
                     /gene="gmd"
                     /locus_tag="VLI90_04155"
     CDS             complement(2208..3236)
                     /gene="gmd"
                     /locus_tag="VLI90_04155"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSV13425.1"
                     /translation="MEQQQRQRKKALITGITGQDGSYLAELLLSKGYLVHGVIRRSSS
                     FNTERIDHLYRDPHDPEARVFLHYGDLVDGTGLREILTRVQPDEVYNLGAQSHVRVSF
                     DQPVYTVAADALGTMNLLESIRDTSLPTKFYQASSSEMYGKVVETPQTETTPFHPRSP
                     YACAKVYSYWQTVNYRESYGMYAVNGILFNHESPRRGETFVTRKITRAATRIKEGLQD
                     KLFLGNLDAKRDWGFAGDYVEAMWLMLQQDKPDDFVIATGETHSVREFLDEVFGILDL
                     DWKKYVVVDPRYFRPAEVDLLLGDASKAKRLLDWTPAITFKALAKMMTEEDWKVARRE
                     RLLADHNH"
     gene            3344..>4479
                     /locus_tag="VLI90_04160"
     CDS             3344..>4479
                     /locus_tag="VLI90_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13426.1"
                     /translation="MADTTPNPAPDPNQSIAQPPPRKKRRLWLKIPLALLILLLLFVL
                     LLPTILSTGMARRFALGQANKNLNGHVEVADWSIGWTGGINAQGIKVFDDAGKQVVQL
                     DHFSTELPLTSAMFGKLHLGKVKIDGLSFDLKADEQGKLNVAKVAKPSNPNEPAPPPA
                     KPSSGKLPDISADVQISNVHGTFTQPGKPAVAVTKLDGNIKIPDINQPITNDFNVGLK
                     VGNGPEGSIKTSGTADVIKDNKVALDTANVQQTVTVNNVDLAAAKPFLAGTGIEDVAG
                     QLGANLALDVKGGKDATLDANVNAGNITAAGPALKGDTFTTKTFTVAVPKLSAAFPQG
                     LDHWQSGRIKAGADAGSAPIAVKLDQAQVTATLDVTPQALLNLK"
BASE COUNT          884 a         1307 c         1392 g          896 t
ORIGIN      
        1 agtctgcatc agcacgacca accgcaattt ccccggccga atgggtagca ccaaaagcgc
       61 ggtctatctg gccagccccc tgaccgtcgc ggccagcgcg ctgacggggt atgtcaccga
      121 tccgcgcgag tatgtttcga cgaagatcga aacgggcatg gcgggggtgc tgtaggacca
      181 ccgtttcaag atggggcgag gatgggaagc acctttcgtg agacgtgggc gagccttcgc
      241 gaagggcttc atcgttttgc ggcgcaggat cgtcagcgac gacgacagct tctattccga
      301 ggccaaagtg atacgaattg gccgttggac acgacgctcg atcggtttaa gcccatagcc
      361 gatccgttaa agcgagcccg gttagtttac gaactgcttc aggagttctc acgggaagct
      421 gttcagatca cttcgcgcgc cgcgcctctt ccggaaggcg atgcactcga attacttgct
      481 cggcaccatg gcttgccatc accgcttttg gattggactc gtagtccgtt tgttgccgcc
      541 tattttgcat tcgaagcagc aggtgcggcc gatcgcgcag tgtgggtgct ggatctgaat
      601 cgcatcccgc ctcccgagtt ctttccagtc gatataatcg atgaccattt gctcctacag
      661 ttcaaccggc gagcactacg gcagcaaagc atttttcttc gggtgaatga ggcaaaacca
      721 ttggagcaga tagcttcaat cgcactgaca aagttcgagc ttccttcatc agagcgtgga
      781 atcgcgctag cggagctcga tgcaatgaat atcaacgcga catccttgtt cgaagatttc
      841 gatggcgtgg cccgcgcggt tcgtactaga atgagctaac gaggcatata tgactgagca
      901 gttcgactgg agtgcgccac cgctttcagc agaagatcag cgactgatct ccgcatattt
      961 gaatgctggt cggccggttg atgatcttgc gtatacgccc gaattcgacc ggatagtgcg
     1021 agctgtaggg ctggagctaa cggatgaaaa caagcacgca ctttaccggc gactcctaac
     1081 gctccgcaag aatggacagc ttccacgagg gttattcgcc gcataaagct gtgtaccgat
     1141 ctttgaagct aatcagcgtg agttgagttg tggaggatgt gggagcgaca cgccgtccgg
     1201 ccaacagcgt catccgttat cccgccgcga gaaccaccag cttctgcgac ggcatatcca
     1261 cctccgccat ccgttccctg cagaagttga gtgtcgcgct ctccacgtcg ctcaaaaact
     1321 tcacgaaaaa ctgccgcgtc tcttcattca tctgcagcga caggatctgg ttcatgccgg
     1381 tgcggacggc gttgaattgc cggcggacgc cggcgtcggg gccgtctaaa cccatcgggt
     1441 cattgctgaa gacgcagctg gcgcgggtcg cgacgatgcg caaacctgcc ttggcggcgc
     1501 tggcttcgaa atcgtagcgg ctgcgcacca gcatccagtt tgtccgcatc gtcgtgcgcg
     1561 gcatgtattc ggtcgtcaca atccgcccgc cgggggcgag gtggttggcg aggttgcgca
     1621 tcgcggtcat gaagcggtcg ggttcgggga tgtggaacag cacgttcatt acgttgatca
     1681 ggtcgaactt ctcgcccagg tccagcgcgg gttcggtgag atcggcggtg cggaacttcg
     1741 tggtgacgct gggaacattc gcgtagcggc gcttcagcag gtcgatggac gactgcgtga
     1801 aatcgacgcc catcagcatg gcgggcttga agcgctggat gaagtagcgg acccaccggc
     1861 cggaaccggc gccgacatct aaaactttcc cgccatcgac gttcaccttc aggtcgcgca
     1921 gcagctgctc gtagatcggg taaagcaccg cgtcttcgac ggggttgctg taggtcagcc
     1981 cgctgtcatc gttggccgcg gcgtcgtcgg attgcttcca ggcttgttcg tagtgggcgc
     2041 ggatgtcttc ctgcatgagc ggcatggggc gagcctccgg atgttatcgg acctcggagg
     2101 atcatcggca ggaggcgatg atgtggtgca gtttttgcgc ctgccgcttg cggcttagca
     2161 tctttctatt ccggcggcgg cgaaaacaga cgctaagccg caagcggcta atggttgtgg
     2221 tccgccagca accgctcgcg ccgggcgacc ttccagtctt cttccgtcat catcttcgcc
     2281 agcgccttga acgtgatggc cggcgtccaa tccagcaggc gcttggcttt gctcgcgtcg
     2341 cccagcagca ggtccacctc agcggggcgg aagtagcgcg gatcgacgac gacgtacttc
     2401 ttccagtcca gatcgaggat gccgaacacc tcgtctaaaa attcccgcac gctatgcgtc
     2461 tcgccggtcg cgatgacgaa atcatccggc ttgtcctgct gcaacatcag ccacatcgcc
     2521 tcgacgtagt cgccggcgaa accccagtcg cgcttcgcgt ccaggttgcc gaggaacagc
     2581 ttgtcctgca aaccttcctt gatgcgcgtg gcggctcgcg tgatctttcg cgtgacgaat
     2641 gtttcgccgc ggcggggtga ttcgtggttg aacagaatgc cgttcaccgc gtacatgccg
     2701 tagctttctc ggtagtttac ggtctgccaa tagctgtaca ccttggcgca cgcatacgga
     2761 ctccgcggat gaaacggcgt cgtttccgtc tgcggcgtct cgaccacctt gccgtacatc
     2821 tcgctgctgg acgcttgata aaacttcgtc ggcaaactcg tgtcgcggat cgactcgagc
     2881 aaattcatcg tgcccagcgc gtcggcggcc accgtgtaga cgggttgatc gaagctgacg
     2941 cgaacgtggc tctgcgcgcc gaggttgtac acctcatccg gctgcacgcg ggtgaggatc
     3001 tcgcgcaggc cggtgccatc gacaagatcg ccgtagtgca ggaagacgcg ggcttcggga
     3061 tcgtgcgggt cgcgatagag gtgatcgatg cgttcggtgt tgaacgagct gcttcggcgg
     3121 atgacgccgt gtaccaggta gcctttgctc agcagcagct cggccaggta tgaaccatcc
     3181 tgcccggtga tgccggtgat caacgctttc tttcgctgcc gctgctgctg ttccatacgc
     3241 cactcttcct tttgcggcga agttaccggc gtcggatggc agtggcaaca atctcgccgg
     3301 ctgttcatcc gcaagtgcga atctcggtat gatggcgcgc gtcatggctg acacgacccc
     3361 caatccggct cccgatccga accaatcgat tgctcaaccc ccgccgcgca aaaagcgccg
     3421 gctgtggttg aagattccgc tggcgctgct gattttgttg ctgctgttcg tgctgctgct
     3481 gccgacgatc ctcagcacgg gcatggcgcg gcgcttcgcg ctgggacagg cgaacaagaa
     3541 cctgaacgga cacgtcgagg ttgccgactg gtccatcggt tggaccggcg ggatcaacgc
     3601 gcagggcatc aaggtattcg acgacgcggg caagcaggtc gtgcagctgg atcatttcag
     3661 cacggagctg cccctgacca gcgcgatgtt cggcaagctg cacctgggga aggtgaagat
     3721 cgacgggctg agcttcgatt tgaaggcgga cgagcagggg aagctgaacg tcgcgaaggt
     3781 ggccaagccg tcgaacccaa atgagcccgc gccaccaccg gcgaaaccga gcagcggaaa
     3841 gctgccggac atttcggccg acgtgcagat cagcaacgtg cacggcacgt tcactcaacc
     3901 cggcaagccg gcggtcgccg tcacgaagct ggacggcaac atcaagattc cagacatcaa
     3961 ccagccgatc accaacgatt tcaacgtcgg gctgaaagtg ggcaacgggc ccgagggttc
     4021 gatcaagacc agcggcacgg ccgacgtgat caaggacaac aaggtcgcgc tcgacacggc
     4081 caacgtgcag cagacggtca cggtgaacaa cgtcgatctc gcggcggcga aaccgttcct
     4141 tgcagggacc ggcatcgaag atgtcgccgg ccaactgggt gcgaacctcg cgctggacgt
     4201 gaagggcggc aaggacgcga cgctcgatgc gaacgtcaac gccggcaaca tcaccgcagc
     4261 cgggccggcg ctgaagggcg atacgttcac gacgaaaacg ttcacggttg ccgtgccgaa
     4321 actgtcggcg gcgtttccgc aggggttgga tcattggcag agcggtcgca tcaaagccgg
     4381 ggcggatgcg gggtcagcgc ctatcgcggt aaagctggat caggcgcagg tgacggcaac
     4441 gctggacgtg acgccgcagg cgctgctgaa tttaaagga
//
LOCUS       DATIMH010000248         3488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_345549, whole genome shotgun sequence.
ACCESSION   DATIMH010000248 DATIMH010000000
VERSION     DATIMH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345549"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VLI90_04165"
     CDS             <1..1099
                     /locus_tag="VLI90_04165"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HSV13427.1"
                     /translation="RHCKITLESPTAATAAYDIEYRTYPSGTVVRSFSPAELASTKPM
                     LDRVQKALLSPGETLPAHVKGTPAQRQQVPPQGTMAIDLEGPAAIRQMSIKIVAANAA
                     DLAQALRSTVLAIDCDGGQTVWCPIGDFFGSGVGLNPFQCWTSKVDKTGLMTCWWVMP
                     FAHTCHVEVRNLGGQPVDVTLGPTFTGDWAWEVWSMYFHANWHQQYPIHTKPRDGTMD
                     WNTISIKGAGVYVGDTLAVHNGARASWSEGDEKISVDGEAFPSHVGTGTADYYGISFG
                     ERSAFFDSPFHAQPRADGNNKPGFVTLTRNRALDAITFSRSLKFDMEIWHWAATDMAY
                     AMTSYWYALPAATSNLAAEVEEAQRGVIFSP"
     gene            1403..1840
                     /locus_tag="VLI90_04170"
     CDS             1403..1840
                     /locus_tag="VLI90_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSV13428.1"
                     /translation="MRLSKRTEYGLRAVVQLARLEPRAFVQSKDLAQQENLPNKFLES
                     ILLALRRGGFLESKVGSGGGYRLSRPPREIAVGELIRRLEGRLTVNQGARAGDISPGE
                     VAVRLLNEKLTNAMDEVLDAMTLEQLMEHVGRAVNEQQEMYYI"
     gene            1931..2743
                     /locus_tag="VLI90_04175"
     CDS             1931..2743
                     /locus_tag="VLI90_04175"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HSV13429.1"
                     /translation="MRFQMFFTATILTRIQNLMGTWGYPVLFGLLFSCGLGFPLPEDV
                     PLILAGYFIAIGKMHIGIAAVCAWLGIIGGDCMLYWFGYKYGLGITKVPIIGKHVTAK
                     RIQYAERLFQRYGTWVVGFGRMFAGIRGAMVIAAGTIRFNIVRFLIADGLAAIVSGGL
                     FMLLGHWAGKKLGDLEALREKIQHYEHYVLGGLILLVALFVLSTIWRKRRHKPPVLEK
                     ALQKVVEKAEKSTVIQLPPSSQQQTGAPTVPPPAPEQTHPTAEQADVAESSR"
     gene            2822..3439
                     /locus_tag="VLI90_04180"
     CDS             2822..3439
                     /locus_tag="VLI90_04180"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HSV13430.1"
                     /translation="MPYQLPPLPFPSDALEPNIDKQTMEIHHDKHHAAYVNNLNAAIA
                     KHPELDGKPLHEILANNLKIVPEDIKTAVRNNGGGHHNHSLFWEILNPAGKPGTSPVG
                     KLGEAINSTFQSFDNFKQQFTTAATTRFGSGWAWLVKRGQKIEVISTANQDSPIMEGA
                     FPIIGLDVWEHAYYLKYQNRRPDYIGAWWNVVNWKVAEDRFGRAQ"
BASE COUNT          702 a         1035 c         1109 g          642 t
ORIGIN      
        1 tcggcactgc aagatcacgc tcgaaagtcc aactgctgcg acggcggcgt atgacatcga
       61 gtatcgcacg tatccttccg gaacagtcgt gcgctcgttc agcccggcgg aactggcgtc
      121 gaccaagccg atgttggacc gcgtgcagaa ggccctgctt tctccgggtg aaacgctgcc
      181 ggcgcacgtg aagggcacgc cggcgcagcg gcagcaggtg ccgccacagg gaacgatggc
      241 gatcgatctc gaaggccccg cggccatccg gcaaatgtcg atcaagatcg tcgccgccaa
      301 cgcggctgat ctagcgcagg cgctgcgatc caccgtgctg gccatcgact gtgacggcgg
      361 gcagaccgtc tggtgcccca tcggtgattt cttcggttcc ggtgtcggcc tcaacccatt
      421 ccagtgctgg acgagcaagg tggacaagac cgggctcatg acctgctggt gggtgatgcc
      481 gttcgcgcac acgtgccatg ttgaagtgcg gaacctcggc gggcagccgg tggatgtcac
      541 gctcggcccg acgttcaccg gcgactgggc gtgggaagtc tggtcgatgt atttccacgc
      601 caactggcat cagcagtacc cgatccacac caagccgcgc gacggcacga tggattggaa
      661 cacgatcagc atcaaaggcg ccggcgtgta cgtcggcgac acgctcgcgg tgcacaacgg
      721 cgcccgcgcc tcgtggagcg aaggcgacga gaagatcagc gtggatggcg aagcgttccc
      781 gtcccacgtc ggcacgggca cggcggatta ctacggcatt tccttcggcg agcgcagcgc
      841 gttcttcgat tcaccgttcc acgcccagcc gcgcgcggat gggaacaaca aaccggggtt
      901 cgtcacgctc acgcggaatc gcgcgctgga cgcgatcacg ttcagccgat cgctgaagtt
      961 cgacatggaa atctggcatt gggccgcaac cgacatggcg tacgcgatga cgtcatactg
     1021 gtacgcgctg cccgcagcga cgagcaacct cgcggccgaa gtggaggagg cgcagcgcgg
     1081 cgtcatcttc tcgccgtgat tcacttactg aagagaattt gaaccacgaa gggcacgaag
     1141 agcacgaaca gggaggaata gctcgtgtct gattttggca gattggctat cgacacattt
     1201 ggaacatcaa tggcgagccc tcgattttcg tacttcgtgt tcttcgtgtt cttcgtgttc
     1261 ttcgtggttc aattcccgtc ccgacgacgt acttcgccga acgcatctcc tccagatcaa
     1321 atcgatcgga attgacgctg tttccgccca cccgtaccat gcccgctcgc gtgcaattcg
     1381 cgtcgcgcgg tggagtgaac ctttgcgcct ttccaagcgg acggaatacg gattacgcgc
     1441 ggtcgtgcag ctggcccggc tcgagccgcg ggcgtttgtg caatccaagg acttggccca
     1501 gcaggaaaac ctgccgaaca aatttctcga atccatcctg ctggcgctcc gccggggggg
     1561 atttctggaa agcaaggtgg gcagcggcgg ggggtatcgg ctatcgcgcc cgccgcgcga
     1621 gatcgcggtg ggtgagttga tccgccggct tgaggggcga ttgacggtca accaaggcgc
     1681 gcgagcgggc gacatttcac cgggcgaagt cgcggtgcga ctgctgaacg agaagctgac
     1741 caacgcgatg gacgaggtgc tcgacgcgat gacgctggag cagctgatgg aacacgtcgg
     1801 tcgggccgtg aatgagcagc aggagatgta ctacatctga attgggaatc agttgctggt
     1861 tgctagttgc tagtgaaagg cgactcgcga catatccgaa ccagcaacga acaaccagca
     1921 actagcactg atgcgcttcc agatgttctt caccgccacc atcctcaccc gcattcagaa
     1981 cctgatgggc acgtggggtt atcccgtgct cttcgggttg ctgttttcct gcgggctggg
     2041 gttcccgctg ccggaggatg tgccgctcat tctcgctggg tacttcattg ccattggaaa
     2101 gatgcacatc ggcatcgccg cggtgtgcgc gtggctcggc atcatcggcg gcgactgcat
     2161 gctgtattgg ttcggctaca agtatgggct cggcatcacg aaggtgccga tcatcggcaa
     2221 gcatgtgacg gccaagcgaa tccaatacgc cgagcgattg ttccagcgct atggcacttg
     2281 ggtggtggga ttcgggcgaa tgttcgcggg gattcgcggg gcgatggtga tcgcggcggg
     2341 gacgattcgg ttcaacatcg tgcgcttcct gattgccgat ggactggccg ccatcgtcag
     2401 cggcgggctg ttcatgctgc tgggccactg ggcggggaag aagctgggcg acctggaagc
     2461 gctgcgcgaa aaaattcagc actacgaaca ttacgtcctg ggcgggctca ttctgctggt
     2521 cgcgctgttc gtcctgtcca ccatctggcg caagcgccgg cacaagccgc cggtgctgga
     2581 gaaggcgctg cagaaggtgg ttgagaaggc ggagaaatcg acggtgatcc agctgccccc
     2641 gtcgtcgcag cagcagactg gggcgccaac agttccgccg ccggcgccgg agcagacgca
     2701 cccgacggcg gagcaagccg acgttgccga atcctcacgt tgacctctct cggccgcagg
     2761 tatacgcttc tgcgctcgaa gcaagacact cgcggcctac cacatagaag gagaaatccg
     2821 catgccttac caactgcccc cgttgccgtt tccatccgat gcgctcgagc cgaacatcga
     2881 caagcagacg atggagatcc atcacgacaa gcaccacgcg gcgtacgtga acaacctgaa
     2941 cgcggccatc gccaagcacc cggagctgga cggcaaacct ctgcacgaga tcctggccaa
     3001 caacctgaag atcgtgccgg aggacatcaa gaccgccgtc cgcaacaacg gtggcgggca
     3061 tcacaatcac tcgctgttct gggagattct gaatcccgcg ggcaaacccg gcacgtcacc
     3121 ggtcggcaag ctgggcgaag cgatcaactc gacgttccag tcgttcgaca acttcaagca
     3181 gcagttcacg accgccgcga caacgcggtt tggatcaggc tgggcgtggc tcgtgaagcg
     3241 cgggcagaag atcgaagtca tttcgaccgc gaatcaggat tcgccgatca tggaaggcgc
     3301 gttcccgatc atcggcttgg acgtgtggga gcacgcgtac tacttgaagt accaaaaccg
     3361 ccggccggat tacatcggcg cgtggtggaa tgtggtgaat tggaaagtgg cggaagatcg
     3421 cttcggtcgc gcgcagtaag acgcgacgta tccgatcttg atgaaacatc gaacgccccg
     3481 gctgcttg
//
LOCUS       DATIMH010000249         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4386373, whole genome shotgun sequence.
ACCESSION   DATIMH010000249 DATIMH010000000
VERSION     DATIMH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4386373"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..482
                     /gene="rplU"
                     /locus_tag="VLI90_04185"
     CDS             171..482
                     /gene="rplU"
                     /locus_tag="VLI90_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSV13431.1"
                     /translation="MYAIIETGGRQHKVTSGEIIRIEREANADEKNITFDRVLFVGGE
                     GSPKIGAPLVAGATVTANVRRQMKGPKIDIMKYKRRKGYHKKQGHRQKLVEVEITGIN
                     L"
     gene            complement(567..1592)
                     /locus_tag="VLI90_04190"
     CDS             complement(567..1592)
                     /locus_tag="VLI90_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13432.1"
                     /translation="MVFSTSLSAATTQPAGDLVSAATAGTKLPHDQAGRLEAALRSAP
                     NDAEQHATLLGLYSLRPADDAGRRLPHVLWTIENRATDPIAGSGYCRIDPEIDPAGYA
                     QGRRLWLQIEAKAPDDLDVLRNASAFMSAEDDVEAERMLAHAAELRPKEGEWPERLAL
                     VYERRALRQRKSHEDRQKSAWNALGQRQRAYLLTHEASHRFGIFVEMPRDAVRAGDYL
                     QAKKLAQQALQMSTMVRDDPAYGAAVNVANIVLGRVALNTGDVDSANAYLQAAGQAPA
                     SPVLASIGPDMSLAKELLKRGQRKTVRDYLDACLRLWPGGATRVRAWIATVDAGETPD
                     FGTQALE"
     gene            complement(1742..>2209)
                     /locus_tag="VLI90_04195"
     CDS             complement(1742..>2209)
                     /locus_tag="VLI90_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HSV13433.1"
                     /translation="IDAILQLNVDRLGELVAHNVRIKARVVEADPFEHGERAHLNFGH
                     TFGHAVEKVSNFAYSHGESVALGMVAATHLAVDLGMLDHGSGDRIMKLIARAGLPTRG
                     MTLDSAAVHEAMAFDKKVKASKLRLILPDRIGHVVIRDDVPAAAVRGAIDSLV"
BASE COUNT          427 a          825 c          593 g          364 t
ORIGIN      
        1 gtggtcgcgg cctcacttgt tgtcgagata ttaccaccgc agcggcgcgc ggcttttcac
       61 caacttttca ccaagtctca ccactagaac ccttgacatt tcccgccgcc tcgtcagaat
      121 tccccgttcg ctcaaaatcg ggcgagcaac gacttcggag ctttacagac atgtacgcga
      181 tcattgaaac cggcggccgg cagcacaaag tcaccagcgg tgaaatcatc cgcatcgaac
      241 gcgaagccaa cgccgacgaa aagaacatca ccttcgatcg cgtcctgttc gtgggcggcg
      301 aaggatcgcc gaagatcggc gccccgctcg tcgccggcgc cacggtgacg gccaacgttc
      361 gccggcagat gaagggcccg aagatcgaca tcatgaagta caagcgccgc aagggctacc
      421 acaagaagca gggccaccgc cagaagctgg tggaagtcga gatcacgggg atcaatctct
      481 aaaagccccc acgccgggtt cggagcgtac tcgcgacgac ccggattatt ctcgcgcgac
      541 tggggcagcg cgcgagacaa tgacgatcac tccaacgcct gcgtcccaaa atccggcgtc
      601 tcccccgcat ccaccgttgc aatccaagct cgcacccgcg tggccccgcc cggccatagc
      661 cgcaggcacg catccaggta atcccgcacc gtcttgcgct gtccgcgctt gagcaactcc
      721 ttggccaggc tcatgtccgg accgatcgac gccagcaccg gcgacgccgg cgcctgcccg
      781 gccgcctgta aatacgcgtt ggccgaatcg acatccccgg tgttcagcgc gacgcgcccc
      841 agcacgatgt tcgcgacatt caccgccgcc ccgtacgccg ggtcgtcgcg aaccatcgtc
      901 gacatctgca gcgcctgctg cgccagcttc ttcgcctgca gataatcccc ggcgcgcacc
      961 gcgtcgcgcg gcatttcgac gaaaatccca aaccgatgag acgcctcgtg cgtcaacaaa
     1021 tatgcccgct gccgctggcc cagcgcgttc cacgcggact tctgccggtc ctcgtgcgac
     1081 ttccgctgcc gcagcgcccg gcgttcatac accaacgcca atcgctccgg ccactcgccc
     1141 tccttcggtc gaagctccgc cgcgtgcgcc agcatgcgct cggcctccac gtcgtcctcc
     1201 gccgacatga aggccgacgc gttccgcaac acatccagat catccggcgc cttcgcctcg
     1261 atctgcagcc acaacctccg cccctgcgcg taacccgccg ggtcgatctc cggatcaatc
     1321 cggcaatacc cgctgccggc gatcggatcg gttgctcggt tctcaatcgt ccacaacaca
     1381 tgcggcagcc gccgcccggc gtcgtccgcc ggacgcagtg aatacagccc aagcagcgtc
     1441 gcatgttgct ccgcatcatt cggcgccgat cgcaacgccg cttcaagccg gccggcttga
     1501 tcgtgcggca acttcgtccc cgccgtcgct gcgctcacca agtcgcccgc gggttgagtc
     1561 gttgccgccg ataacgacgt ggagaacacc attgcgcaca cgaccgcgcg agcgaattgg
     1621 cgagcgatga accacatgcg tttcttgaaa gccagagaat ccgccgcttg cggcttcgca
     1681 aatcgagcag caccgcttgc gaagccgcca acggcggtgc gcgcgagcgc gcgcggcccc
     1741 gtcacaccaa tgaatcaatc gccccccgca ccgccgccgc cggcacgtca tcgcgaatga
     1801 cgacatgccc aattcgatcc ggcaggatca gccgcagctt cgatgccttc accttcttat
     1861 cgaacgccat cgcctcatgc accgccgcgg aatcgagcgt catgccacgc gtcggcagcc
     1921 ccgcccgcgc aatcagcttc atgattcgat cgcccgatcc atgatcgagc atccccagat
     1981 ccaccgccag atgggtcgcc gccaccatgc ccagcgccac gctttccccg tgcgaatacg
     2041 cgaagtttga aaccttctcc accgcatgcc caaaggtatg gccgaagttc aggtgagcgc
     2101 gttcgccgtg ttcaaaagga tccgcctcga cgactcgcgc cttgatccgc acgttgtgcg
     2161 cgaccagctc gcccagccga tcaacgttca gctgcaggat cgcgtcgat
//
LOCUS       DATIMH010000250         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1789572, whole genome shotgun sequence.
ACCESSION   DATIMH010000250 DATIMH010000000
VERSION     DATIMH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789572"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VLI90_04200"
     CDS             <1..776
                     /locus_tag="VLI90_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13434.1"
                     /translation="SRIGNWFKRGPRPSNGDMLDPDNTGDTRLANSGVGHPVVRRMKK
                     RDQAIAMQEGFNALTNLMTTIKDNLNDQGRRQDELLKILSHLPQALQAIPESNRLQGE
                     TLRTIHARLEQQNEQQQLIAEILNKVAESGNEQRKTVADVRDRVDSMADHEAKIADNL
                     SSVGAAMQTVSRNSQASAQVLEQLRDNINNRDGQLERILHKQAARFTTMLAIAIFLSV
                     AAIGAVAIIGYQMMNKPAMNAPTTAQPVQAPAEIRANPT"
     gene            993..1346
                     /locus_tag="VLI90_04205"
     CDS             993..1346
                     /locus_tag="VLI90_04205"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSV13435.1"
                     /translation="MELTRAVLSELLKALRSNPKTGSEKRVQPRVGLRLRARVFTTTS
                     IDRPVEVWLRDISAGGLGLLSSQPFARGDTIKIILADSEDDQLPCTVAYCRRVGNRLF
                     QIGLKFAAGQGSLAA"
     gene            complement(1521..2219)
                     /locus_tag="VLI90_04210"
     CDS             complement(1521..2219)
                     /locus_tag="VLI90_04210"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSV13436.1"
                     /translation="MAADVQQRMIPQEPPKVPGIDLAAVYVPCFELGGDFYDFIPLQG
                     DNTGLAVADVAGKGVPASLIMASVRAALRAHVDNVYYLYEVIRRINVMVYRDTKPSEF
                     VTLFYGVLDARSKRFTYCNAGHPPGLLLREGKVIELASDNMVLGVDPNEEYKQSIIDL
                     KSGDILLLYTDGLPDAMNFKQETFGRQRLAEAFKKGGETAEIVSQNILWELRKFVGMS
                     KRTDDVTMIVARVD"
     gene            complement(2232..3242)
                     /locus_tag="VLI90_04215"
     CDS             complement(2232..3242)
                     /locus_tag="VLI90_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSV13437.1"
                     /translation="MSGLDADHLKLTDFMDLPTLQEIQDNFAAIANVRAIITDAEGNV
                     LTQAQPTREFLQRQRALAAAEESRPAPQKEGAEYVAPITINNQRLGTLRMSIEPGAVA
                     GVDEARLAAIATKFNIDLKQAKSLATQLTRSRTARPAAIQFLFLLANAIARLCYQEFQ
                     LRQRINELTAVYGVTMMLADARDLQKVLQRTVQVVADLMDTKACSIRLIDRDQDELVI
                     KAVYNLSDEYLAKGPVRLSQADIDQIALSSQGFEFVRNMATDPRVRYPQESAREGIVS
                     MLSVGMRYKGRAVGVLRVYTDQEQSFSPLKIDLLKAVAAQAAAAIENARLVEERLASQ
                     AL"
BASE COUNT          642 a         1099 c          999 g          593 t
ORIGIN      
        1 tgtcgaggat cggcaactgg ttcaagcgcg gcccgcgccc cagcaacggt gacatgctcg
       61 accccgacaa caccggtgac acgcggctgg ccaacagcgg cgtgggccac ccggtcgtcc
      121 gccggatgaa aaagcgcgat caagccatcg ccatgcagga gggcttcaat gcgctgacga
      181 acttgatgac gaccatcaag gacaacctga acgaccaggg ccgccggcag gatgagctgc
      241 tgaagatcct gtcgcacctg ccgcaggcgc tgcaggcgat tcccgaaagc aaccgcctgc
      301 agggcgaaac gctgcggacg attcacgcgc gactggaaca gcagaacgag cagcagcagc
      361 tgattgccga gatcctgaac aaggtcgccg agagcggcaa cgagcagcgc aagacggtgg
      421 ccgacgtgcg cgaccgcgtg gattcgatgg cggaccacga ggcgaagatc gccgacaacc
      481 tgtcgagcgt cggcgccgcc atgcagaccg tcagccgaaa ttcgcaggcc agcgcgcagg
      541 tgctcgagca gctgcgcgac aacatcaaca accgcgatgg ccagctcgag cgcatcctgc
      601 acaagcaggc ggcacggttc acgacgatgc tggcgatcgc gatcttcctg tcggtggccg
      661 ccatcggggc cgtggcgatc atcgggtatc agatgatgaa caagcccgcg atgaacgcgc
      721 cgacgacggc ccaaccggtt caagcgccgg cggagatccg cgccaatccg acataacgcg
      781 ccatcgcgaa aactgccgga caatcagcga gattatcgct cccgccacgg gtgtctgccg
      841 gcgaatgcgc ctgcgattct gtcccgcctc aggaatcccg ccgccaaaag aataccaccg
      901 ttcgcccttg cctttggtcc gattcaaacc gaaatcaaag gtagggagtt gccgatcgcg
      961 gccgatggtg tccgcgaaga cggctttcaa ccatggaact cactcgggcc gtactaagcg
     1021 agctactcaa ggcgctgcga tccaacccga agacggggtc ggaaaagcgc gtacagccac
     1081 gcgtgggatt gcgcctgcgg gcgcgcgtgt tcaccaccac ctcgatcgac cggccggttg
     1141 aagtctggtt gcgcgacatc tccgccggcg ggctcgggct gctctcgtcg caaccgttcg
     1201 cgcgcggcga cacgatcaag atcatcctcg ccgattccga ggacgaccag ctgccctgca
     1261 ccgtcgcgta ctgccggcgc gtcggcaatc gactgttcca gatcggcttg aagttcgccg
     1321 cggggcaggg aagtttggcg gcgtgaatca ccctttatcc tccagcgcag gcgtactcgc
     1381 cggagtcgaa ggatttagcc gaggagttgg agaaacctcg ttcgcgagaa gcgccggacc
     1441 gccgcttgcg gtttagcatc ggtcgcctct tgcacgcaga acagatgcta agccgcgagc
     1501 ggcgggacga ttgttgagaa tcaatccacc cgcgccacga tcatcgtcac gtcgtccgtg
     1561 cgcttcgaca tcccgacgaa cttccgcagc tcccacaaaa tattctgcga cacgatctcc
     1621 gccgtctccc cgcctttttt gaacgcttcc gccagccgct gccggccgaa ggtttcctgt
     1681 ttgaagttca tcgcatccgg caagccatcg gtgtacagca gcaggatgtc gccgctcttt
     1741 aggtcgatga tcgactgctt gtattcctcg ttcggatcga cgccgagcac catgttgtcg
     1801 ctggcgagtt cgatcacttt cccctcgcgc agcagcagcc ccggcgggtg accggcgttg
     1861 caataggtga accgcttgct gcgcgcatcg agcacgccgt agaacagcgt gacgaattcg
     1921 cttggtttgg tgtcgcggta gaccatcacg ttgatccgcc ggatcacttc gtacaggtaa
     1981 taaacgttgt ccacgtgagc gcgcaatgct gcgcggaccg aggccatgat caagctcgcc
     2041 ggcacgcctt tgccggcgac gtcggcgacg gcaaggcccg tgttgtcgcc ctgcagcggg
     2101 atgaaatcgt agaagtcgcc acccagctcg aagcacggca cgtacaccgc cgccaggtcg
     2161 atgcccggca ccttcggcgg ctcctgcggg atcatgcgct gctgcacgtc cgccgccatc
     2221 tgcacctgct tttacaaggc ctgcgacgcc aaccgctctt ccaccagccg ggcgttttcg
     2281 atcgccgccg ccgcctgcgc cgccaccgct ttcagcaggt cgatcttcaa cggactaaaa
     2341 ctttgctcct ggtccgtgta gacgcgcagc acgcccacag cacgcccctt gtagcgcatt
     2401 cccactgaaa gcatcgaaac gatgccttcg cgggccgatt cctgcggata tcgcacgcgc
     2461 gggtcggtcg ccatgttccg cacgaactca aatccctgcg acgacagcgc gatctgatcg
     2521 atatctgcct gactcaatcg caccgggcct ttggccaaat actcgtcgct caggttgtaa
     2581 acggctttga tgaccaactc gtcctgatcc cgatcgatca ggcggatcga gcaggccttg
     2641 gtgtccatca aatctgccac gacctgcacc gtccgctgca gcaccttctg caggtcgcgc
     2701 gcatcggcga gcatcatggt gacgccatag acagccgtca gttcgttgat gcgctggcgc
     2761 agctgaaatt cctggtaaca cagccgcgcg atcgcgttgg ccagcaggaa caaaaactga
     2821 atggccgccg gcctggcggt gcgcgatcgc gtcagttgcg tcgccagcga cttcgcctgt
     2881 ttcaaatcga tgttgaactt ggtggcgatc gccgccagcc gcgcttcatc gacgccggcc
     2941 accgcgccgg gttcgatcga catgcgcagc gtgccgagcc gctggttgtt gatggtgatc
     3001 ggcgcgacgt attccgcgcc ttccttctgc ggcgccggcc gcgattcttc cgccgccgcc
     3061 agcgcccgtt gacgctgcag aaattcccgc gtgggctggg cctgcgtcaa cacattgccc
     3121 tcggcatcgg tgatgatcgc gcgcacgttc gcgatggccg cgaagttatc ctggatctcc
     3181 tgcaacgtcg ggagatccat gaaatcggtc aactttaggt gatcggcgtc aagcccagac
     3241 attcacgcgc tccgtcgccc gcggttcggc gcgacgcgcc gcggcgactg gcgattgtag
     3301 aggttgagac ggcgcaatca acgtgagcca atg
//
LOCUS       DATIMH010000251         5327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3521692, whole genome shotgun sequence.
ACCESSION   DATIMH010000251 DATIMH010000000
VERSION     DATIMH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3521692"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..556
                     /locus_tag="VLI90_04220"
     CDS             101..556
                     /locus_tag="VLI90_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13438.1"
                     /translation="MASNATDFARHLGSYEPTLLAYMATPFIFSATPGQFSPALFRAF
                     CDCYVDGFLATFQAIRQRNPNLTRVFYPSSSAVEQTPANMGEYAAAKRAAESLCQYLE
                     SVHRKLRIHTPRLPRLATDQTASLLQAEMPDAAELMLEHLRQTLLSEEE"
     gene            721..867
                     /locus_tag="VLI90_04225"
     CDS             721..867
                     /locus_tag="VLI90_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13439.1"
                     /translation="MDLSEGPIISFFRRHPWLHLVAVLLLCVGTIVILLGGYRTVGLV
                     YKAF"
     gene            867..1916
                     /locus_tag="VLI90_04230"
     CDS             867..1916
                     /locus_tag="VLI90_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13440.1"
                     /translation="MTELTPTIDADDRRAARGHVRLALIVLALLAGIFVSADVCERHD
                     QWLLHRRLVQWRLTNGSYFDGRYFDNPEERLLYHSLPSHDFSRGGVWFFGSSPTQQAV
                     LPWEFPADIRPRVENFSMAGANHNNERRFMRCLIDHHGLLAAGGDKSLVVIELLFGSA
                     CHDRTPVQQYMQDVWDGYFTRYGMYYFGADGEILPTEMTPLECTFKSQSLRSHVFLSW
                     LLRHENNWLKPPEHAITSEILRYWSDRFGPNWEAGMDAEIAELSAAIDYLQARHANVA
                     GLYFPQRQWARDYRPAIRYRQRVMALMAAKSVQVIDLRDSFDESEFADSVHLNYVGSM
                     KLQPRLIELAQRQLQ"
     gene            2059..2418
                     /locus_tag="VLI90_04235"
     CDS             2059..2418
                     /locus_tag="VLI90_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13441.1"
                     /translation="MDELPEKIESDVRRHARRAAETLRFWTKLALMILLALLLGVLLP
                     SACHERAAFHAAERAYEQASMAYAKRAMAGQPVDPDIAKEKDRAEQRYVAASRRYRYY
                     FAWLLRDPALTAHQDDH"
     gene            2492..4144
                     /locus_tag="VLI90_04240"
     CDS             2492..4144
                     /locus_tag="VLI90_04240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HSV13442.1"
                     /translation="MPRATSIDRNQLRQIAKQKFGYDELRPGQEQTIRSIVDGHDALS
                     IMPTGSGKSAIYQIAGLLIDGPTVIVSPLIALQKDQKDSIEEAGIAEAAVVNSTQRVG
                     ERREAFEGLDGGTLEFLFLSPEQLANEETREHLMANRPSLFVVDEAHCLSEWGHDFRP
                     EYARLGSVIEALGHPRVLALTATASPNVREEIVQRLGMRQPRVIVWGFDRPNIWLSVE
                     ACPDEEIKNRVLIGRATDLPKPMIVYVGTHRHAEEISQQLCDAGLGSACYHGGMKKAD
                     RDAVQDQFMADGHEIIVATNAFGMGVDKPNVRTVIHYDIPESIDAYYQEIGRAGRDGE
                     PSRAILLYRAEDLGMRRAQAAGPKLTETIVEQVAEAVADRNDPVAIKDLKEETDLPAS
                     KVAAAVNRLEEVGAVKITADGEVKPAGKNDPTDSVEDAVREQDAYRRYRLGRVELMKD
                     YAESRDCRRRYLLNYFGEAAGGPCGHCDNCDAGIVQKSAPERATDEQHPFALKSRVVH
                     RKYGEGVVMRYDKEKVEVLFNEAGPKSLVTDYLVEHELMRAV"
     gene            4195..>5327
                     /locus_tag="VLI90_04245"
     CDS             4195..>5327
                     /locus_tag="VLI90_04245"
                     /inference="COORDINATES: protein motif:HMM:NF027641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13443.1"
                     /translation="MPSLVIDTIIASTLMISIAAAQQPATSPKAANPPLIVAHYMSWY
                     QTPAVSGSWGFWQVNRANIDTKYWHWPDKNTPAGGPDIASVYCPIIGPYDSADPDTCE
                     YHILLAKLCGIDAFVADWYGPDASQEHPYDNIGFLAMKRAAERLDFKVMICWEDRSMF
                     PPISKAVTSRRDAVERGKQMIAYLAKEWFASPAYLKIDGHPVLTDYTYVQSNGTMSTP
                     ALDADEWNQVLQSTQPTQRGAMLIHDWHRHFKSPEFGGYTSVVPWGDTYHGNADRADT
                     FWPAAEHAVRDGDNRFLSGAVLPGFDNRGCGGWGTGGAIGITDRRDGAKFRATWADVM
                     RHDVRFIQIATWNDFNEGGTIEPVRQIVLHEDAPAPGYGYREL"
BASE COUNT         1008 a         1689 c         1695 g          935 t
ORIGIN      
        1 cccgccacgc cgggttcgga ggtactccga cgacccggat tcttctggcg cgagtaaaga
       61 acaagaatcc aggtcgtcgg gagtaccttc cgaacccggc gtggcgagta atgcaaccga
      121 cttcgcgcgt catttagggt cgtacgagcc cacgctcctc gcgtacatgg cgacgccgtt
      181 catcttctcc gccaccccgg ggcaattttc gccggcgctg ttccgcgcgt tttgcgattg
      241 ttacgtcgat ggtttcctcg ccacgtttca agcgattcgc cagcggaacc cgaatctgac
      301 gcgcgtgttt tatccatcgt catccgcggt cgagcagacg ccggcgaaca tgggcgaata
      361 cgccgcggcg aagcgggcgg cggagtcgtt gtgccaatat ctggagagcg tgcaccgcaa
      421 gcttcgcatt cacacgccgc ggttgccgcg gcttgcaacg gaccagacgg cgagcctgct
      481 gcaggcggag atgccggacg cggcggagtt gatgctggag catcttcggc agacgctttt
      541 gagcgaggaa gaataggcac gtaattcgcg cgcgccgaga tcgtcacagc cgcaggcgac
      601 agccagcggg ggctcgggta atcgagccgg actggtgttc gaagcccccc gctggctgtc
      661 gcctgcggct acgaaagaga ttcaccaccg tcgcgcgcga cgtgttcaac tggaacgacc
      721 atggatttat ccgaaggccc catcatctcg ttcttccgac ggcacccctg gctgcacctg
      781 gtcgcggtgc tgctgctgtg cgtcggcacg atcgtcattt tgctcggtgg atatcgcacc
      841 gtcggtttgg tttacaaggc gttttgatga cggagctgac gcccacgatt gacgcagacg
      901 accgccgcgc cgcccgcggg catgtcaggc tcgcgctgat cgtgctcgcg ctgctcgcgg
      961 gcattttcgt ctcggcggac gtctgtgagc gacacgacca atggctgctg catcgccggc
     1021 ttgtgcagtg gcggctcacc aacggcagct atttcgacgg ccgctatttc gacaacccag
     1081 aagagcgcct gctctatcac agcctgccga gccacgactt ctcccgcggc ggggtgtggt
     1141 tcttcggttc gtcgccgacg caacaagcgg tgctgccgtg ggagttcccc gccgacatcc
     1201 gcccgcgcgt ggaaaacttt tcgatggccg gcgcgaatca caacaacgag cggcgcttca
     1261 tgcgctgcct gatcgatcac cacggcctgc tggccgccgg tggggataaa tcgctcgtcg
     1321 tgatcgagct gctcttcggc tccgcctgcc acgaccgcac gcccgtgcag caatatatgc
     1381 aggatgtgtg ggacggttat ttcacgcgct acggcatgta ctacttcggc gcggacggcg
     1441 aaatattgcc gacagaaatg accccgctcg agtgcacgtt caagtcgcaa agcctccgct
     1501 cgcacgtctt cctcagctgg ctattgcggc acgagaacaa ttggctcaag ccgcccgagc
     1561 acgcgatcac atcggagatc ctacggtatt ggtccgaccg cttcggcccc aattgggaag
     1621 cgggcatgga cgcggaaatc gcagaattat ccgccgcgat cgattatctc caggcgcgtc
     1681 acgccaatgt cgccgggttg tatttcccgc agcggcagtg ggctcgcgat taccggccgg
     1741 ctattcgata tcgccagcgc gtgatggcgc tgatggcggc gaagtcggtg caggtgatcg
     1801 acctgcgcga ttcattcgat gagagcgagt ttgccgattc ggtgcatctc aattatgtgg
     1861 ggtcgatgaa gctgcagcca cggttaatcg agctggcgca gcggcagttg caatgatcac
     1921 aaaacccgcg gctctgctgt gggacgcgtt agacgaattt tctacgccag tcccagggca
     1981 gagccatacg ctttgttgca gacgcgctgc gcttccattc gcgcatcgca cgcgcgttgc
     2041 tagcatcgat tcgtccccat ggacgaacta ccggagaaga tcgaaagcga tgtcaggcgc
     2101 cacgcgcgcc gcgcggccga gacattgcgc ttctggacca agcttgcgct gatgatcctg
     2161 ctcgccctgc tgctgggcgt gttgctgccc agcgcctgcc acgagcgcgc ggcgttccac
     2221 gccgccgagc gcgcgtatga gcaggcgtcg atggcgtacg cgaagcgggc gatggcggga
     2281 cagccggttg atccggacat cgcaaaggaa aaggaccgag ccgagcagcg gtacgtcgcc
     2341 gccagccggc gctatcgcta ctacttcgcg tggctgctgc gcgatccggc gctcaccgcg
     2401 catcaggacg accattagcc gccgcaaatt cgcgcgatga tgtgcgactg taatcgcgcg
     2461 ccggcggcac atcggttgca cttctccgtt catgccgcgc gccacttcaa tcgaccgcaa
     2521 ccagctccgt cagatcgcaa aacaaaagtt cggctacgac gagctgcgcc ccgggcagga
     2581 gcagacgatc cgctcgatcg tcgacgggca cgatgcgttg tcgatcatgc cgaccggctc
     2641 cggcaagtcc gcaatttacc agatcgcggg gctgctgatc gatggcccca ccgtgatcgt
     2701 ctcaccgctg atcgcgctgc agaaggacca gaaggattcg atcgaggaag ccggcatcgc
     2761 cgaggcggcg gtggtgaatt ccacgcagcg cgtcggcgaa cgccgtgagg cgttcgaagg
     2821 cctggacggc ggcaccctgg aattcctgtt cctctcgccc gagcaactcg ccaatgaaga
     2881 aacgcgcgaa cacctgatgg ccaatcggcc gtcgctgttc gtcgtggacg aagcccactg
     2941 cctcagcgaa tggggtcacg actttcggcc ggaatacgcc cggctcggca gcgtgatcga
     3001 agcgctcggt cacccgcgcg tgctggcgct aacggcaacg gcatcgccca acgtgcgcga
     3061 ggagatcgtg cagcggctgg gcatgcgcca gccgcgcgtc attgtctggg ggttcgatcg
     3121 cccgaacatc tggctcagcg tcgaggcgtg tcccgatgaa gagatcaaaa accgcgtgct
     3181 gatcgggcgc gcgacggatc tgcccaagcc gatgatcgtc tacgtcggca cgcatcgcca
     3241 cgccgaggag atctcgcagc agctttgcga cgcgggcctg ggcagcgcgt gctatcacgg
     3301 cgggatgaag aaggccgatc gcgacgccgt gcaggaccag ttcatggccg acgggcacga
     3361 gatcatcgtc gccaccaatg cgttcggcat gggcgtggac aagccgaacg tgcgaactgt
     3421 cattcattac gatattccgg aatcgatcga cgcgtattac caggagatcg gccgggccgg
     3481 gcgcgatggc gagccgtcgc gcgcgatcct gctgtatcgc gccgaggacc tgggcatgcg
     3541 ccgcgcgcag gcggcgggac cgaagctcac ggaaacgatc gtcgagcaag tcgccgaggc
     3601 ggtggcggat cgcaacgacc cggtcgccat caaggacctg aaggaagaaa ccgatctgcc
     3661 cgcgtccaaa gtcgcggcgg cggtgaaccg gctggaagaa gttggcgccg tgaagatcac
     3721 cgcggatggc gaagtcaaac ccgcgggcaa aaatgacccg actgacagcg ttgaagacgc
     3781 ggtgcgcgag caggatgctt accgacgata tcgactgggc cgggtcgagc tgatgaagga
     3841 ctacgccgaa tcgcgcgatt gccgccggcg ctatctgctc aactatttcg gcgaagccgc
     3901 cggtggtccc tgcggccact gcgacaactg cgatgccggc atcgtgcaaa agagcgcgcc
     3961 tgagcgagcg accgatgagc aacacccgtt cgcgctcaag agccgggtcg tccacaggaa
     4021 gtacggcgaa ggcgtggtca tgcgatacga taaggaaaag gtcgaggtgc tgttcaacga
     4081 ggcggggccg aagagccttg tgacggatta cctcgtcgag cacgagctga tgcgggcggt
     4141 gtaacggggc gagcacaagg ttaagatgtc gagcgtctga ccgatcgccg atccatgccg
     4201 tcgcttgtca ttgataccat catcgcatcc acgctcatga tctccatcgc cgccgcgcaa
     4261 cagccggcga cgtcgccgaa ggctgcgaac ccgccgctca tcgtcgcgca ttacatgtcg
     4321 tggtaccaga cccccgccgt ctccgggtcg tggggattct ggcaagtcaa tcgcgcgaac
     4381 attgacacca aatattggca ctggccggac aagaacacgc cggcgggcgg gccggatatc
     4441 gcatcggtct attgcccgat catcggtccg tacgactcgg ccgaccccga cacctgcgaa
     4501 taccacatcc tccttgccaa gctttgcggc atcgatgcgt tcgtcgccga ctggtacggg
     4561 cccgatgcgt cgcaggagca tccgtacgac aacatcggct tcctcgcgat gaagcgggcg
     4621 gccgaacggc tcgatttcaa agtgatgatc tgctgggaag accgctcgat gttcccgccg
     4681 atttccaaag cggtgacgtc gcgacgagat gcggtcgagc gcggcaagca gatgatcgcc
     4741 tacctggcga aagagtggtt cgcatcgccg gcgtatctga agatcgacgg ccacccggtg
     4801 ctgaccgact acacctacgt ccagtcgaac ggcacgatgt cgaccccggc gctggatgcc
     4861 gacgaatgga accaggtgct gcaatccacg cagccgacgc agcgcggggc gatgctcatt
     4921 cacgactggc atcggcattt caagtcgccg gaatttggcg gctacacctc cgtcgtcccc
     4981 tggggcgaca cgtatcacgg caacgccgac cgcgccgaca ccttctggcc cgccgccgag
     5041 cacgccgtgc gcgacggcga caatcgcttt ctctccggcg ccgttctccc tggcttcgac
     5101 aaccgcggtt gcggcggctg gggcaccggt ggcgccatcg gcatcaccga ccgccgcgac
     5161 ggcgccaagt ttcgcgccac ctgggccgac gtgatgcggc acgacgtgcg cttcatccag
     5221 atcgccacgt ggaacgactt caacgaaggc ggcacgatcg aaccggtgcg ccagatcgtg
     5281 ctgcacgaag acgcgccggc gccgggttac ggttatcgcg aattgga
//
LOCUS       DATIMH010000252         3742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_57186, whole genome shotgun sequence.
ACCESSION   DATIMH010000252 DATIMH010000000
VERSION     DATIMH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57186"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VLI90_04250"
     CDS             complement(<1..187)
                     /locus_tag="VLI90_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13444.1"
                     /translation="MNLPDFSIESWRTRTTPAHDEELTIFHSIIDRSGLIQRLPSHNQ
                     LRIEVCDAVPAGTKLAAG"
     gene            complement(274..2784)
                     /locus_tag="VLI90_04255"
     CDS             complement(274..2784)
                     /locus_tag="VLI90_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HSV13445.1"
                     /translation="MNFNDRLKSIAILAAAVLAFTTFALHAEPPQSAATAAATAEVDR
                     RTEELLAQLTLDEKLQLLGGVDSFYTRPIDRLNIPRLKMSDGPVGTRNDGPTTAYPAG
                     VALAATWDPALAEREGQSLGRDARARGDHFLLGPGVNIYREPQNGRNFEYFGEDPYLA
                     GQIAVGYIRGVQSQGVAACVKHFACNNQETDRMTIDARVDERALHEIYLPAFEAAVKS
                     GHAWSVMASYNRINGDYATANHYLLTDVLKDSWGFTGVLMSDWGATHDTLGPMTAGMD
                     LEMPSPKFLNAAKIKPLIDDGPVSIAMIDDKVRRLLRVMLAMHWLDRPQKDSSIPLED
                     PSSNDTALAVARESIVLLKNQGNLLPLDRNKPQTIAVVGPTAAHYTAGGGSSMAKPFH
                     PITVLEGLQKIAGDRVKIVHIPFISLGGNDLTAFADASQYESPGLAAEFFNNRDLTGK
                     PVTRADHTINFNWHDEEMPIDGITTRSFSARWTGNIRAAESGPYVFAVNSDDGSRVKL
                     DGKTIIDDWTNHATRVRTATVNLTAGETHDVVVEYYNSRGGASVQFGWGKPPALLSPD
                     DRNMLAQADAVIACVGTSESEGADRAYELPEDQNQLITDAAGLNPKTIVILNAGGNVA
                     MADWIERVPALLNAWYPGQAGGQAIAETIFGDNNPSGHLPDTFEKQWPDAPASGHYPG
                     KDGKVEYAEGIYVGYRWFDSRKIEPRFPFGHGLSYTTFTIDNLKITPVSGGDSHDQVA
                     TVTVTNTGNKRGATVAQLYVRPHTTGIDRPLQELKAFARVELEPGQNKTITLPLNSRS
                     FAYWDVDTHAWKSPAGEYEIAVGTSSRDIACVGRVELK"
     gene            complement(2805..>3742)
                     /locus_tag="VLI90_04260"
     CDS             complement(2805..>3742)
                     /locus_tag="VLI90_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13446.1"
                     /translation="PGGTLLDDQGVLTIYGGGSEIFATSDSFRFLSQPAAQPMDRSTR
                     VLCMLDTDLYAKAGLMARWSMEPNSPFAMVNVFPDGSVAMCSRSSKGATTTEKHVQSG
                     VPIGEVVLRLTVEGGHATAYFRRGDEKWRTIAAAAVPADAAFDQGYAVCAHRESGFTV
                     ARFAPPEVSVATTRPTETSLSDDWQMSGAWRRSGDGGSAVFNADASGRLWQEIPVQPG
                     RRYRFTVDASGGDTTPTTNPTRLVELRLEGTLDDQPVALNAASMMASHTMSIIGTAIG
                     SHLRVVIVSPAQGKIAVHRASLADLSSANDACTDQ"
BASE COUNT          621 a         1170 c         1205 g          746 t
ORIGIN      
        1 gccccgccgc cagcttcgtc cccgcgggca cggcatcgca gacttcaatt cgaagctggt
       61 tatgcgacgg caaacgctgg atgagtcccg atcggtcgat gatcgagtgg aagattgtta
      121 gctcctcgtc gtgcgccggt gttgttcgcg tgcgccatga ttcgatggag aagtcgggga
      181 ggttcatctc tcaaagccca cgattaggcc gtgggaccga ttgcccggac gcacatgaca
      241 gcggaggctc ttgtcgtggc ggccatagcc ccgtcatttc aactcaactc ggccgacgca
      301 cgcgatgtct cggcttgatg tgcccacggc aatctcatat tcgcccgcgg gggatttcca
      361 cgcgtgggta tcgacatccc aatacgcgaa cgatcggcta ttgagcggca gcgtgatcgt
      421 cttgttctgc cccggctcga gttcgacgcg ggcaaacgcc ttcaactcct gaagcggccg
      481 atcgatgccg gtggtgtgcg ggcgaacgta taactgtgcg accgtggcgc cacgcttatt
      541 gccggtgttg gtcaccgtga cagtcgcgac ctggtcgtgc gaatcgccgc cgctgaccgg
      601 cgtgatcttc aggttgtcga tcgtgaaggt cgtgtacgac aacccgtggc cgaacggaaa
      661 ccgcggctcg atctttcggc tgtcgaacca gcgatagccg acgtagatgc cttccgcgta
      721 ttcgaccttt ccgtcctttc cgggatagtg gccggacgcg ggggcgtcgg gccattgctt
      781 ctcgaacgtg tcgggcaggt ggccgctggg gttgttgtca ccgaagatcg tctcggcgat
      841 cgcctgccca ccggcctggc cgggatacca cgcattcagc agcgccggca cgcgctcgat
      901 ccaatccgcc atcgccacgt ttcccccggc gttcaggatg acgatcgtct tcggattaag
      961 ccccgcggcg tcagtaatca gctggttctg atcttcgggc aactcgtacg cgcgatcggc
     1021 accttccgat tcactcgtgc cgacgcaggc gatgacggca tccgcctgcg cgagcatgtt
     1081 gcgatcatcg ggcgacagca gcgccggcgg tttgccccag ccgaactgca cgctggcgcc
     1141 gccgcgggag ttgtaatatt cgacgacgac gtcgtgcgtt tccccggcgg tgaggttgac
     1201 ggtcgcggtg cgcacgcggg tggcgtggtt ggtccagtcg tcgatgatcg tcttgccgtc
     1261 gagcttcacg cgcgagccat cgtcgctgtt gactgcgaag acatacggcc cgctctcggc
     1321 cgcgcggata tttccggtcc atcgcgctga gaacgaccgc gtcgtgatgc cgtcgatcgg
     1381 catctcctca tcgtgccaat tgaagttgat cgtatggtcg gcgcgcgtga caggttttcc
     1441 ggtgagatcg cggttgttga agaactccgc ggccaggccc ggcgattcat attgcgacgc
     1501 atcggcgaac gccgtcaggt cgttgccgcc caggctgatg aaggggatgt gtacgatctt
     1561 gacgcgatcg ccggcgattt tctgaagtcc ctccagcacg gtgatcggat ggaatggctt
     1621 agccatcgag ctgccaccgc cggcggtgta atgggcggcg gtggggccga cgacggcgat
     1681 cgtctgcggt ttgtttcgat ccagcggcag caggttgccc tggttcttca gcagcacgat
     1741 cgactcgcgc gccaccgcca gcgccgtgtc attgctcgag ggatcctcca acggaatcga
     1801 gctgtccttc tgcggccgat cgagccagtg catcgccagc atcacgcgca gcaaccggcg
     1861 caccttgtcg tcaatcatcg cgatgcttac ggggccgtcg tcgatcagcg gcttgatctt
     1921 cgccgcgttc agaaatttcg gcgacggcat ctccaggtcc atccccgcgg tcatcgggcc
     1981 tagcgtgtcg tgcgtggcgc cccagtcgga catcagcacg cctgtgaacc cccacgaatc
     2041 tttaagcaca tcggtgagca ggtaatgatt cgcggtggcg tagtcgccgt tgatgcggtt
     2101 gtaggaggcc atgacggacc atgcgtgccc gcttttcacg gctgcctcga aggcggggag
     2161 atagatctcg tgcagcgcgc gttcgtccac gcgcgcatcg atcgtcatgc ggtcggtctc
     2221 ctggttgttg cacgcgaaat gcttgacgca ggcggcgaca ccctgcgact gcacaccgcg
     2281 gatgtaaccg acggcgatct ggccggcgag gtatggatct tccccaaaat attcgaaatt
     2341 ccggccgttt tgcggctcgc ggtagatgtt tacaccgggg ccgagcaaaa aatgatcgcc
     2401 gcgcgcacgg gcgtcacgtc ccagtgactg gccttcgcgc tcggccagcg ccggatccca
     2461 cgtcgcggcc agcgcgacgc ccgcggggta ggcggtggtg gggccgtcgt tgcgcgtgcc
     2521 gaccgggccg tcgctcatct tcaggcgcgg gatgttcagg cggtcgatcg gccgggtgta
     2581 gaacgaatcg acgccgccga gcaactgcag cttttcgtcc agcgtgagct gggcgagcag
     2641 ttcttcggtg cggcgatcaa cttctgccgt cgccgccgcg gttgcagctg actgcggcgg
     2701 ttcggcatgt aatgcgaatg tagtaaatgc aagcacagcg gcggcgagaa tcgcaatgga
     2761 cttcaatcga tcgttgaaat tcacaatcac tctcctggag atgttcattg gtcagtgcag
     2821 gcgtcgttgg cgctgctcag gtccgccagc gatgcgcgat gcaccgcgat cttaccctga
     2881 gccgggctga cgatcacgac gcgcagatga gagccgatgg ccgtgccgat gatggacatc
     2941 gtgtgcgaag ccatcatcga cgccgcgttc agcgcgacgg gctggtcgtc cagcgtgcct
     3001 tcgagccgga gttcgacaag gcgggtggga ttcgtcgtcg gcgtggtgtc gccaccggac
     3061 gcatcgactg tgaagcgata gcgtcgcccc ggttgtaccg ggatctcttg ccacaatctc
     3121 ccgctggcgt cagcgttgaa gacggcgctg ccaccgtcgc cggatcgtcg ccatgcgccg
     3181 gacatctgcc aatcgtcgct caggcttgtc tcggtcggac gcgtcgttgc cacggacact
     3241 tccggaggcg cgaagcgcgc gacggtgaag ccggactcgc gatgggcgca taccgcgtag
     3301 ccctgatcga acgccgcgtc cgccggcacc gccgcggcgg cgatcgtccg ccatttctcg
     3361 tcaccgcggc ggaagtacgc ggtggcgtgg ccaccttcaa ccgtcagtcg aagcacgacc
     3421 tccccgatcg gcactccgct ctgcacgtgt ttctccgtgg tcgtcgcgcc cttgctcgat
     3481 cggctgcaca tcgcgacgga accgtcggga aagacattga ccatcgcgaa cggcgagttg
     3541 ggctccatgc tccatcgggc catcagcccg gccttcgcgt aaagatctgt gtccaacatg
     3601 catagtacgc gcgtcgagcg atccattggc tgcgccgcgg gctgcgacaa gaagcgaaac
     3661 gaatcgcttg ttgcgaagat ctccgaccca ccgccgtaga tcgtgaggac gccttgatca
     3721 tccagcaacg taccgccggg tg
//
LOCUS       DATIMH010000253         5397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4097761, whole genome shotgun sequence.
ACCESSION   DATIMH010000253 DATIMH010000000
VERSION     DATIMH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4097761"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="VLI90_04265"
     CDS             complement(<1..1821)
                     /locus_tag="VLI90_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV13447.1"
                     /translation="MIARIIEFSVRNRLMVLLLTAMLVVGGIWAALHISLDAVPDLSD
                     VQVIITTDYAGQAPNVVEDQVTYPLTTAMMGIPHAKVVRGISMFEQSFVYIIFDDSTD
                     LYWARSRVLEYLNFVKDRLPANVEPKLGPDATGVGWVYQYVVYPGYFDPAHPKGVWHD
                     VENDRWYAEPSQAPADRQAKLVKVRAFDKPGTSPVSGKALAGSSQNLASLRSLQDWYL
                     RYQLTAVPNVAEVAPIGGFVKEYQVVLKPERLLAYKLPISRIMMAIQRSNNDVGGSVV
                     EMAENEYMIRSRGYLNGLNDLGKVPVGVGEGGTPIMLADVATLQIAGEERRGIGEWNG
                     VGEAVGGVIVARFGANAYQVIHDAKAKLAELEEGLPPGVTIKVAYDRSDLIERSIHTL
                     RHTLVEEIIVVGLVCIIFLLHARSELVAIFVVPSSVLASLLVMHLMGINANIMSLGGI
                     AIAIGVVVDSAIIMVENAHKHLDREDERVHAGLPPRDRGEVIMEAAKEVGPSLFFSLL
                     IITVSFVPVFVLGGESGRLFKPLAFTKTFAMAAASILSITIIPVLMVYFITSRVLPKR
                     WGWKWNTLLTLSAMLVPAAILWMVADLVPALRPWQAWLAGG"
     gene            complement(2041..3906)
                     /locus_tag="VLI90_04270"
     CDS             complement(2041..3906)
                     /locus_tag="VLI90_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSV13448.1"
                     /translation="MKNGSWNSTAWLIALAIGALAAVVWMISPHHTGGIDGNVASATT
                     TTSGQPSSSGEHLWTCGMHPQVLLPHPGNCPICGMKLTPVKDQDHDDHPAAGKSQRKI
                     AYWWDPMLGASSVSDHPGKSAMGMDLVPVYDEQLQSGPGVRIDPTVVQNIGVETAAVT
                     RGPLNVTVRAVGVLMVPTPGLHDVTLKISGFVQKLYADTEGAAVVKGDVLFDLYSPEL
                     IVAGQELSAAEQSLKSLDANASDAQRAEANAMVESAKSKLRLWDIDERDIEAMVSSGG
                     QVKTVPIRSPADGYVTDKMIVQGSAVQAGMKLMRIVDQRSMWLDVQVYPGQAERVAMG
                     QPVRTTIDGLPGKTFNGKITFIAPQVDQNTLAQTVRTTLDNPDHELRPGMFATANITT
                     QPVQDTVMAPREAVIDTGARQIAFVMDATNPGHFQPRNVRMGIAGDDDKVQIVEGLAP
                     GDQVVTSGQFLMDVESRTTEAIQKLRSAGMAGGGAKSAAATTAPAELTLAHCSMWNTD
                     WLQVGDGIANPYAGSLMLTCGNVLRRVSGPANGTPLAGVIDAYLKIEQGLDADKIDAN
                     ATAAIKRAADLLTGDRYVSLRQASDRLATAADLTTAREAFKNATDALIPLLEEHH"
     gene            4086..4661
                     /locus_tag="VLI90_04275"
     CDS             4086..4661
                     /locus_tag="VLI90_04275"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSV13449.1"
                     /translation="MPMARVVASVLLLAMLAIGCSSSRRATAELWVEDHGGVVAGKSR
                     AQTRLQTVSRPLITACCGRKISVQILATDAVTAYSLWDGHVFVTRGLMNHLDNAELQA
                     AVAHELGHLLSDGHVQTIASLRGCCDDPDCEVRADAAGAQLLRAQGLSPDAMPSMLQK
                     IVRFGALSPTCTRALQHRIGLLSESLDDPPR"
     gene            complement(4740..>5397)
                     /locus_tag="VLI90_04280"
     CDS             complement(4740..>5397)
                     /locus_tag="VLI90_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13450.1"
                     /translation="ADDAVAKRLAKLRTMPMDLSNLERSMRQMIPPAEAATSPRLQIW
                     TRWARPLRAVAAGIILVAAILILMLTTSGGPVVASTTDMAKFHDDLVSGRVPVTRVDS
                     VQAANATLASEWAASPQVPDIPNEHVMACCMRSVRNKRMACVLLKGEGEPVTMTVANA
                     SEMKPPASPVVSRDGVQYHVEAVGDLNMIMTERHGRWVCLVGKVPSGRLIDLAASLQF
                     "
BASE COUNT          930 a         1833 c         1620 g         1014 t
ORIGIN      
        1 cccgccggcg agccacgcct gccaggggcg aagcgcgggc acgaggtcgg cgaccatcca
       61 gaggatggcc gcggggacca acatcgccga cagcgtcagc agcgtgttcc acttccagcc
      121 ccagcgcttg ggcagcacgc ggctggtgat gaagtagacc atcagcaccg ggatgatggt
      181 gatcgacaag atcgacgccg ccgccatggc gaacgttttg gtaaacgcca gcggtttgaa
      241 gagccggccg ctttcaccgc cgaggacgaa gaccggcacg aagctgacgg tgatgatcaa
      301 cagactgaag aacaggctcg ggccgacttc tttggcggcc tccatgatga cctcaccgcg
      361 atcgcgcggc ggaagccccg catgcacgcg ctcgtcttcg cgatccaaat gtttgtgcgc
      421 gttctccacc atgatgatcg cgctatcgac caccacgccg atggcgatcg cgatgccacc
      481 caggctcatg atgttcgcgt tgatgcccat gaggtgcatc acgagcaggc tggccagcac
      541 gctggagggc acaacgaaga tggcgaccag ttcgctgcgc gcgtgcagca gaaagatgat
      601 gcagaccagg ccgacgacga tgatctcttc gaccagcgtg tggcgcagcg tatgaatcga
      661 ccgctcgatc agatcgctgc ggtcgtaagc caccttgatc gtcacgcccg gcggcaggcc
      721 ttcttccagc tcggccagtt tcgccttggc atcatgaata acttgatagg cgttggcgcc
      781 gaagcgcgcg acgatgacgc cgccgaccgc ttcaccgacg ccattccatt caccgatgcc
      841 gcggcgttct tcgccggcga tctgcagcgt cgccacatcc gccagcatga tcggtgtgcc
      901 gccttcaccg acgcccaccg gcaccttgcc caggtcgttc agcccattga gatacccgcg
      961 gctgcggatc atatattcgt tctcggccat ctcgacgacg ctgccgccga cgtcgttgtt
     1021 cgaccgctgg atggccatca tgatccggct gatcggcagc ttgtaggcca gcaaccgctc
     1081 gggcttcagt acgacttgat attctttaac gaacccgccg atgggcgcga cctctgccac
     1141 gttcggcacc gccgtcagct ggtaacgcag ataccagtcc tgcaggctgc gcaggctcgc
     1201 caggttctgg ctcgacccgg ccagcgcctt gccactcacc ggcgatgtgc cgggcttgtc
     1261 gaacgcgcgc accttcacga gcttcgcctg tcgatccgcc ggcgcctgcg atggctcggc
     1321 gtaccaccgg tcgttctcca cgtcgtgcca cacgcccttt ggatgagccg gatcgaagta
     1381 accgggatag acgacgtact gatacaccca acccacgccg gtcgcgtcgg ggccgagctt
     1441 cggttccacg ttcgccggca gccgatcctt cacgaagttc agatattcca gcacccggct
     1501 gcgcgcccaa tagaggtcgg tgctgtcgtc gaaaatgatg tagacgaacg actgctcgaa
     1561 catgctgatc ccgcgcacga ccttcgcgtg cgggatgccc atcatcgcgg tggtcagcgg
     1621 gtacgtgacc tgatcttcga cgacgttggg cgcctgcccg gcgtaatcgg ttgtgatgat
     1681 gacttgaacg tcagacaggt ccgggaccgc gtcgaggctg atgtgcaggg ccgcccagat
     1741 cccgccgacg accagcattg cggtgagcag cagaaccatc agacggttgc ggacggagaa
     1801 ttcgatgatg cgcgcgatca cggccgaccc ccgggcgctg cggcgcgtcc cgctgtcatc
     1861 ctgagcgcgt atcgagagac tggttgagtc cttaatagcc caggccgaaa ggccggggat
     1921 cggaccgaaa gtggcacgtt ttgcagtccg atccccggcc ttccggcctg ggctattacg
     1981 gccgctacac ttcctgcttt cattgtcata attggtactc gggcattcat tgggggtggc
     2041 tcaatggtgt tcttccagca gcggaatcag cgcatccgtc gcgttcttaa acgcctcgcg
     2101 cgccgtggtg agatcggcag cggtcgctag ccggtcgctc gcttgccgca gcgacacata
     2161 tcgatcgccc gtcaataaat ccgccgctcg cttgatcgcg gccgtggcgt tcgcgtcgat
     2221 cttgtcggcg tccagtccct gctcgatctt caaataagca tcgatcacgc ccgccagcgg
     2281 tgtgccgttc gcgggacccg acacgcgccg cagcacgttg ccgcaggtga gcatgagcga
     2341 accggcgtac gggttggcga tgccgtcgcc gacctgcagc cagtcggtgt tccacatcga
     2401 gcagtgcgcc aaagtcagct ccgccggcgc cgtcgtcgcc gccgcgctct tggccccgcc
     2461 gccggccatg ccggcgcttc gcaacttctg aattgcctcg gtcgttcggc tctcgacatc
     2521 catcaaaaac tgcccgctgg tcaccacctg atcgcccggc gccagacctt cgacgatctg
     2581 caccttgtcg tcatcgccgg caatgcccat gcgcacgttg cgcggctgga agtgaccggg
     2641 gtttgtcgcg tccatcacaa atgcgatttg ccgagcgccg gtgtcaatga cggcttcgcg
     2701 cggcgccatc accgtgtcct gcaccggctg cgtggtgatg ttggcggtcg caaacatgcc
     2761 cggccgcagc tcgtgatcgg ggttgtccaa cgtcgtgcgg accgtctgcg ccagcgtatt
     2821 ctgatccacc tgcggtgcga taaacgtgat cttgccattg aacgtcttgc ccggcagccc
     2881 gtcgatagtc gtccgcaccg gctggcccat cgccaccctc tccgcctggc ctggataaac
     2941 ctgaacgtcc agccacatcg agcgctggtc cacgatccgc atcagcttca tccccgcctg
     3001 caccgccgag ccttgcacga tcatcttgtc cgtgacatat ccatcagcag ggctccggat
     3061 cggcacggtc ttcacctgcc caccgctgga taccatcgct tcgatatccc gctcgtcgat
     3121 gtcccacagg cgcaacttgc tcttggcgga ttcaaccatc gcgttcgctt ccgcccgctg
     3181 cgcatcgctc gcgtttgcat ccagcgattt cagcgactgc tcggccgccg acaattcctg
     3241 gcccgcgacg atcagctccg ggctgtacag gtcgaacagc acatcgcctt tgacgaccgc
     3301 cgccccttcg gtgtcggcgt acagcttctg aacgaaaccg ctgatcttca gcgtcacatc
     3361 gtgcaacccc ggcgttggca ccatgagcac acccaccgcg cgcaccgtca cgttcagcgg
     3421 cccgcgcgtc accgccgccg tctccacgcc gatgttctgc accacggttg gatcgatccg
     3481 cacgcccggc cccgactgca gctgctcgtc gtacacggga accaaatcca tccccatcgc
     3541 gctcttgccg ggatgatcgc tcacgctgga cgcgccgagc attggatccc accaatacgc
     3601 gatcttccgc tggctcttgc ccgcggcggg gtgatcatcg tggtcctggt ccttcaccgg
     3661 cgtgagcttc attccgcaga tcgggcagtt gcccgggtgc ggcagcagca cctgcgggtg
     3721 catcccgcag gtccagaggt gttcgccgct ggatgaaggt tgacccgagg tggtggtggt
     3781 tgccgatgcg acgttgccgt cgataccgcc ggtgtgatgc gggctgatca tccaaaccac
     3841 cgccgccagc gcgccgatgg cgagtgcgat cagccatgcc gttgaattcc atgaaccatt
     3901 cttcatcgcc cgattccttc cggcatgccc tcaaagcagt taacgacggg atggacggaa
     3961 aaggttccat cgctgccggc gcgcaataaa tgggcagcat gatccgcaag gtttgatatt
     4021 tgcgcacgcg gcgtgcgtga aatggctgga attgcagctg gcaccggcat tgttattgcc
     4081 gtcgcatgcc aatggctcgg gtggttgcga gtgtgttgtt gctggcgatg ctggcgatcg
     4141 gttgctcatc gtcgcggcgg gcaacggctg aattatgggt cgaggaccac ggcggtgtcg
     4201 tggccggcaa aagcagagct cagacgcggc tgcagacggt ctcgaggccg ctgatcaccg
     4261 cctgctgcgg ccggaagatc agcgtgcaaa tcctcgcgac cgacgccgtc accgcgtaca
     4321 gcctgtggga tggacacgtc ttcgtgaccc gcggcttgat gaaccatctc gacaacgcgg
     4381 agctgcaggc cgcggtggcg cacgaactgg gtcacctgct cagcgacggt cacgttcaaa
     4441 ccatcgcgag cctgcgaggc tgctgcgacg atccggattg tgaggttcgc gccgacgcgg
     4501 ccggcgcgca gctgcttcgc gcccagggtc tgtcgcccga cgcgatgccg agcatgctgc
     4561 agaagatcgt gcgcttcggc gcgctctccc caacctgcac ccgcgcgttg caacatcgca
     4621 tcgggctgtt gagcgagtcg ctcgatgacc cgccgcgtta ggagggatgt cggaaactca
     4681 ccgtgacacg ggcgtccgtg tcacgattta gcttctgaat tcgcgcctca agttccagtt
     4741 caaaactgaa ggctcgccgc caggtcgatg aggcggccgc tcggcacctt gccgaccagg
     4801 cacacccagc gtccgtgccg ctcggtcatg atcatgttta gatcgcccac cgcctcgacg
     4861 tgatactgca cgccatcgcg cgagaccacc ggtgacgccg ggggtttcat ctccgatgcg
     4921 ttcgcgaccg tcatggtcac cggctcgcct tcgcctttca gcagcacgca cgccatccgc
     4981 ttgttgcgca ccgaccgcat gcagcacgcc atcacgtgtt cgttgggaat atccggcacc
     5041 tgcggcgacg ccgcccactc cgacgccagc gtcgcgttcg ccgcctgcac cgaatccacc
     5101 cgcgtcaccg ggacgcgccc gctcaccagg tcgtcgtgga atttggccat gtccgtcgtc
     5161 gaagcaacga ccggcccgcc ggatgtcgtc aacatcagga tgagaatcgc ggcgacaagg
     5221 ataatccccg cggcaacggc gcgcagcggc cgcgcccagc gcgtccaaat ctgcaaccgc
     5281 ggcgaagtcg ccgcctcggc cggcgggatc atctgccgca tcgaccgctc gaggttcgac
     5341 agatccatcg gcatcgtgcg cagcttcgcc aatcgcttcg ccacggcgtc atctgcg
//
LOCUS       DATIMH010000254         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1789796, whole genome shotgun sequence.
ACCESSION   DATIMH010000254 DATIMH010000000
VERSION     DATIMH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789796"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VLI90_04285"
     CDS             complement(<1..1220)
                     /locus_tag="VLI90_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HSV13451.1"
                     /translation="MRRSSSVLWSTTALTAMIAAAAPAAAQTVPPPPNPGATAQQNPA
                     EPAQNADAPQSPAAANGTGNVADVPIVVTGLRRSLQSARNIKRNSDQIVDAVVAEDIG
                     KLPDITVSDTAARIPGVQVERSGGEANRVLLRGLDNTYYTTTYNGREIFTAETRSVAL
                     QDFPAGAIAAVEAFKTSTANLVEPGIAGLLNVRSRRPFDFSGSEISGSVWALHPNQSR
                     DTSINGNLLLSDRWEMGNGAEFGALVNFSYTRIHYKDSVRRHGFFIANLDGGRSPDWP
                     EIHYNEGNRWRPSANAALQYRSGDLELYAEGLWQGYRERVTDRMWAQPLWSCGGAATY
                     SNIQFRPGTNEILSGTVQNPGCPGSAWGFKGATHRETNTYQFAAGGSYDAGALRITAD
                     LARTYSHFQLRTESV"
     gene            complement(1345..2481)
                     /locus_tag="VLI90_04290"
     CDS             complement(1345..2481)
                     /locus_tag="VLI90_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSV13452.1"
                     /translation="MINRFNAENPDIHIDVNVVAWPGYPQLTAQMAARDPPDLVTMHE
                     GVISDYALKGLLEPVDWYLARAGISTNDFTDASRAGVTIGAHEYGLPWDTHGGLFHVN
                     TGLFAKAGLMRGGKPVLPTSAAELIQQARQFEQRTGKPYLIQSLVGDPAGAARNLYTY
                     MMAQGAVLFPDPKHVKLDTPVGVRVATFFRDINAQHLGTLNMDTPGAIAAFINGEGGI
                     YPTGTWMIGSFEQEAETPGRPLYKNYAVFPYPRLWGQRVEYVSGHSWVVPSRKRTPRQ
                     RDAIARFFRFMAAHNYDWARTGHIPAFKSVLADPRFMALPHRADIAPLAAMGRQLPPY
                     VQRQSAIEGLVGEEAAAAFTGQKSVSQALADAERRVDALLGHLN"
     gene            2512..2706
                     /locus_tag="VLI90_04295"
     CDS             2512..2706
                     /locus_tag="VLI90_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13453.1"
                     /translation="MIDVDAAAVLGAALAEEALEVDVRLVVASRAAAERKDEQKRGGD
                     PPHRSAAPAIARTICFWKTI"
BASE COUNT          484 a          860 c          884 g          493 t
ORIGIN      
        1 acgctttcgg tacgcagctg gaagtggctg taggtgcgcg ccaggtcggc ggtgatccgc
       61 agcgcgcccg catcgtaact gccgccggcg gcgaactgat aggtgttcgt ctcgcggtgg
      121 gtcgcgccct tgaagcccca cgcgctgccg gggcagcccg ggttctggac cgttccgctc
      181 aggatctcat tcgtgccggg acggaactgg atattcgaat aagtcgcagc gccgccgcag
      241 ctccacagag gctgcgccca catgcgatcg gttacgcgct cgcgatatcc ctgccacagg
      301 ccttcggcgt aaagctcgag atcgccactg cgatactgca atgcggcgtt tgccgacggg
      361 cgccagcgat tgccttcgtt atagtggatt tcgggccagt cgggcgaacg gccgccgtcg
      421 aggttcgcga tgaagaagcc gtgccggcgg accgaatcct tatagtggat acgcgtgtag
      481 gagaagttga ccagcgcgcc gaactcggcg ccgttgccca tctcccagcg gtcgctgagc
      541 agcaggtttc cgttgatcga cgtgtcacgc gactgattcg ggtgcagcgc ccacaccgat
      601 ccggaaattt ccgagcccga aaaatcgaac ggacggcgcg agcggacgtt cagcaggccg
      661 gcaatccccg gctcgaccag gttcgcagtc gatgtcttga aggcttccac ggcagcgatc
      721 gcgccggcgg gaaagtcctg cagtgcaacc gatcgggttt cggcggtaaa gatttcgcgg
      781 ccgttgtacg tcgtcgtgta ataggtgttg tcgaggccgc ggaggagcac acgattggct
      841 tcacccccgg agcgttcgac ctggacgccg gggattcgcg cggcggtgtc cgacacagtg
      901 atgtcgggca gcttgccgat gtcttccgcc accaccgcgt cgacgatctg gtcgctgttg
      961 cgcttgatgt tgcgcgcaga ctgaaggctg cgcctgaggc cggtgacgac aatgggaaca
     1021 tcggcaacgt tgccggtacc gtttgccgct gcgggcgact gcggcgcgtc cgcattctgc
     1081 gccggctcgg ccggattttg ctgcgccgta gcgccgggat tgggcggcgg cgggacagtt
     1141 tgcgcggcag ctggagcagc tgcggcaatc atcgccgtca atgccgtagt cgaccacaaa
     1201 accgagctgg aacgccgcat atagagcccc ctcctgcgac caaagtacag ccgtaaaccc
     1261 gaacagcctg cacattacta ttacgccggt tggaaaagtt acaagaagca atagggcaac
     1321 actgatgcag gttattttcg acgctcagtt gaggtggccg agcagcgcgt cgacgcgacg
     1381 ctcggcatcg gccagcgcct gactgacgct cttctgcccg gtgaatgccg cagccgcttc
     1441 ctcgccgacg agcccttcga tcgcgctctg ccgctgaaca taaggcggca gctgccgacc
     1501 catcgctgca agcggcgcga tatccgcgcg gtgcggcaag gccatgaacc gcggatcggc
     1561 aagaaccgac ttgaacgcgg ggatgtgccc cgtccgggcc caatcataat tgtgcgccgc
     1621 catgaacctg aagaagcgcg cgatcgcgtc ccgctgccgc ggcgttcgct tgcgcgacgg
     1681 cacgacccag ctgtggccgc tcacatattc gacgcgctgc ccccacaggc gcggataggg
     1741 aaagactgca tagtttttgt acagcggccg cccaggcgtc tccgcttcct gctcgaacga
     1801 gccgatcatc cacgtgcccg tcggataaat gccgccctcg ccgttgatga acgccgcaat
     1861 cgcgcccggc gtgtccatgt tgagcgttcc caaatgctgc gcgttgatgt cgcgaaagaa
     1921 agtcgcgacc cgcaccccga ccggcgtgtc cagcttgacg tgcttcgggt ccggaaacag
     1981 caccgcccct tgcgccatca tgtaggtgta gaggttgcgc gccgcgccgg ccggatcgcc
     2041 gacgaggctt tggattagat aaggcttgcc ggtgcgctgc tcgaactggc gcgcctgctg
     2101 aatgagctcg gcggcgctgg tgggaagcac cggcttgccg ccgcgcatca gccccgcctt
     2161 ggcgaacaag ccagtgttca catgaaagag cccgccgtgc gtgtcccacg gaagcccgta
     2221 ttcatgcgcg ccgatcgtca ctcccgcgcg actcgcgtcg gtaaagtcgt tcgtgctgat
     2281 gcccgccctg gcgaggtacc agtcgaccgg ctcaagcagc cccttcagcg cataatcgct
     2341 gatcacgcct tcatgcatcg tgacgagatc gggcggatcg cgggcagcca tctgcgcggt
     2401 gagctgcgga tagccgggcc aggcgacgac gttgacgtcg atgtgaatgt cggggttctc
     2461 ggcgttgaag cggttgatca aagtggtgat gatcccgcat tcgccttcgg catgatcgac
     2521 gtcgatgctg ccgccgtact cggcgccgca ctcgccgaag aagcgctgga ggtagatgtg
     2581 cgtcttgtgg tcgcttcgcg cgcagctgcc gagcgcaagg atgagcaaaa gcgcggcggc
     2641 gatccgcctc atcgcagcgc cgcacccgcc atcgcgcgca cgatctgttt ctggaagacg
     2701 atatagacga tgaagatcgg g
//
LOCUS       DATIMH010000255         8176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4966685, whole genome shotgun sequence.
ACCESSION   DATIMH010000255 DATIMH010000000
VERSION     DATIMH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4966685"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VLI90_04300"
     CDS             complement(<1..452)
                     /locus_tag="VLI90_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13454.1"
                     /translation="MKSKESRTVRGPDFNTMTTITDTDLELLEAYLDGEVSGTEADAL
                     QQRLRAEPELSAAMDDLRSQRAIRQSLWQSLEPDQKTADQFAWRIRGAIETQRQTPAR
                     RKWDTWQFARFGSAAAACVVLGFFVGWVGRGHHANVSSVVSPPSHNESA"
     gene            complement(568..1074)
                     /locus_tag="VLI90_04305"
     CDS             complement(568..1074)
                     /locus_tag="VLI90_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV13455.1"
                     /translation="MTTEQLFEKAVRENSRRLLAIARGIVGTRTSPEDVVQQAVMNLF
                     QHRARYDWREPGGLLRRAVVNEALRILRQPRMAAMEEDYHGGSAAPPDADMDERETVA
                     RVRQAIDRLPPNFKAALVLCEYENMPYAQIAESLDASVPQVKTWIFRARRQLAEMLKD
                     LAPANRSS"
     gene            1736..3175
                     /locus_tag="VLI90_04310"
     CDS             1736..3175
                     /locus_tag="VLI90_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSV13456.1"
                     /translation="MSHSHPHPATVGQLKETDYRPMSVKDELRKNLIRKLKAGEQLFP
                     GILGYRDSVIPQIVNGILSKHDLLFLGLRGQAKTRILRMLPDLLDEWMPVLSGVEIND
                     DPLSPMTKTGQRIVAEQGDDAKIEWVHRSDRYQEKLATPDVTIADLIGEIDLVKHAEG
                     RYLSDESTMHYGMIPRSNRGLFAINELPDLAPRIQVGLFNVLEERDVQIRGYPIRLNL
                     DVCLVFSANPEDYTNRGRIVTPLKDRIGSVIRTHYPETVQEGIEIAQQNAWVDRDAQP
                     SGSGVRVDTPAFMTEIVEQVVRLARSSPHVSQASGVSVRTSIANLETVTSNAERRGII
                     TGEKRVVPRICDLNYLVASCRGKIEMTLAEEDGAEDKLVRSLIGEAVKTVFAKYADTD
                     SYDHISEQFKSNLTFPAGDDLSADEFVANMKAVNGLHKTAVALSREMELDGGDHAILA
                     SVGEFLLEGLYVNNRLSKYNAKGKTFFKR"
     gene            complement(3388..4089)
                     /gene="tsaB"
                     /locus_tag="VLI90_04315"
     CDS             complement(3388..4089)
                     /gene="tsaB"
                     /locus_tag="VLI90_04315"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HSV13457.1"
                     /translation="MPRGLAIETSGRLGSIAVIDGDAVVAEEVFPHGLKHAAEIVPII
                     DRLTRSRGWSPRDVEHLYVSAGPGSFTGLRIGVTLAKVMALATGVKLVAAPSLQVLAR
                     NAPADAQHLIVVLDAKRDQIFTARFERAGSQWLEREPAKLDDLTSMLARSPRPVHLLG
                     EGIPYHQQFIPPDDGSVIVTPPERWRARASAVAEIGATFAAAGEFNDPFTLTPIYVRR
                     PEAEEKYELAQKNRA"
     gene            complement(4169..4684)
                     /locus_tag="VLI90_04320"
     CDS             complement(4169..4684)
                     /locus_tag="VLI90_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13458.1"
                     /translation="MDDQRSQLPPRTWSSAWSIPLMCLGLGVIAACVLVPQAEANKRL
                     AAERDKLKRDLAYVDTQISVNSEFLKNVGGDASLAERLAQRQMKVIREGSSVLELRGR
                     HVGSKREEMSPFMLVSVAPPPAPAPYAPPAGILGSLCRDARYQLYVSGLGMLMVATGL
                     VLGASATPRRV"
     gene            complement(4772..6061)
                     /gene="eno"
                     /locus_tag="VLI90_04325"
     CDS             complement(4772..6061)
                     /gene="eno"
                     /locus_tag="VLI90_04325"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSV13459.1"
                     /translation="MSVSIEFIQGRQILDSRGNPTIEVDVILEDGTMGRAAVPSGAST
                     GEYEAVELRDDDKKVYVGKGVLKAVDNVNSKIAPELIGLDARDQEAIDQLMIEMDGTP
                     NKAKLGANAMLGVSLAVAKAAAKSSGLPLYRYLGGPAAKTLPVPMMNILNGGKHADNN
                     VDFQEFMIQPWGFESFSDGLRAGVEIYHALKKVLHKNKMSTAVGDEGGFAPDLKDNED
                     ALKVIGEAVTAAGYEFGKQVFVALDPAASELWDKEKKGYKFFKSAPDKVLAPEAMVDY
                     WAKWVEKYPIRSLEDGLAENDWDGWKKLTDRLGKKIQLVGDDLFVTNVKFLEKGIKTG
                     TANSVLVKVNQIGTLSETFATVNMAIRNGYSAVLSHRSGETEDATIADIAVATNCGQI
                     KTGAPARSDRVAKYNQLLRIEEQLGEHAQYGAQFWNK"
     gene            complement(6365..6829)
                     /locus_tag="VLI90_04330"
     CDS             complement(6365..6829)
                     /locus_tag="VLI90_04330"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSV13460.1"
                     /translation="MRLSAEMFNDIVEALRSDRKGDRDQRIEPRVGMAGEANLVSICD
                     DGKRRISRVRIRDISRSGIGFCTSTKFAKDQRVIIQLQSFQGEPIWLICVAAHSRRLE
                     ADRFSIGVRIKQVMRSEQVHRVEQQLGQAAVQLLLDPAARASAARISKAILA"
     gene            complement(7192..>8176)
                     /locus_tag="VLI90_04335"
     CDS             complement(7192..>8176)
                     /locus_tag="VLI90_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13461.1"
                     /translation="NAGTPAGVVIVGGAMADSSLNSSVPIRVMRVASWSDTANNSSIS
                     APLIATLISLGGFNAALNLSGSGSALPTLGVAQVVGAIGGDSWAINGRANTIVAGSIT
                     SAWSGTLAGLLNVIVDRGGSFASNLSAGGIGTMLVIGDVNGGAAITAASANVIRVTGS
                     VANSTITMNGPASATPSLRAMIVGNAITGSTIATAGDLTALIASSLAGSTITAGVDGG
                     VTTIDAVTPGNIGSHAIRSLRLTARTAGAFAGSTVIAHTITTAALGDVNTNNNGTPEG
                     VAAVAFTSLTATVGGIRVAPSRTQLQDAGQLSAYFSDKGVNLGDFRVDIVT"
BASE COUNT         1445 a         2637 c         2583 g         1511 t
ORIGIN      
        1 gccgattcat tgtgtgacgg cggcgagacg accgatgaga cattcgcatg atgcccgcgg
       61 ccaacccaac cgacgaagaa gccgagcacg acgcacgcgg cggcggcgga gccgaagcgg
      121 gcgaactgcc aggtgtccca cttgcggcgg gccggcgtct gacgctgtgt ttcgatcgcg
      181 ccgcggatgc gccaggcgaa ttgatcggcc gtcttctggt cgggttcgag tgattgccag
      241 agggactggc ggatggcgcg ctgcgaccgc aggtcgtcca tggcggccga caattcgggt
      301 tcggcgcgca ggcgctgctg cagcgcgtcg gcttcggtgc cgctcacctc gccgtcgagg
      361 taggcttcca gcagttcgag atcggtgtcc gtgatcgtcg tcatcgtatt gaagtccggg
      421 ccgcggacgg tccggctctc cttggatttc atttcctgca atcgatcgtt gtctgcttcg
      481 tcattttcta aagatacttc cgtctgtgac ggacgcgcgc tcagcgttcg gctctgcggg
      541 ccgccgctaa aaaatcgggt tcgacattta cgaactccga ttggcgggcg ccaggtcctt
      601 caacatctcc gccagttgcc ggcgggcacg gaagatccag gtcttcacct gcggcacgct
      661 ggcatcgagc gactcagcga tctgcgcgta cggcatgttc tcgtactcgc acagcaccag
      721 ggccgccttg aagttcggcg gcaggcgatc gatcgcctgc cgcacacggg cgaccgtttc
      781 gcgctcgtcc atgtccgcat cgggcggggc ggccgatcca ccgtggtaat cctcctccat
      841 cgccgccatg cgcggctggc ggaggatccg cagcgcctcg ttcaccaccg cccggcggag
      901 caacccgccg ggttcgcgcc aatcataccg ggcgcgatgc tgaaacaggt tcatcaccgc
      961 ctgctggacg acgtcttctg gcgacgtgcg cgttccgaca attcctcggg cgatcgccag
     1021 cagccgacgt gaattctcac gaacagcctt ttcaaacagt tgctcggtgg tcacaccgtt
     1081 gggatctttc gcctccaacg gagccggcgg ggactgcggt ggcgcagacg ccgccggacc
     1141 gacacccgaa gggtgaacac cggcattgtc agcttcaggc tgttggctct tctcatccat
     1201 cacatgcgcc gcgggaagcg aagtttcgca gaatgttacg gcaataacac cggctgcgtc
     1261 acgactttat ggtccggcag gtgatggagc aaattgcgcc gaatcggatc agagctgatt
     1321 ccgtcgctcc gcgcgtggca tgcggcatcc ccacaacttg cgtagcggta gccccgcagg
     1381 ggcacggcgc cgccggcccg tggtgcgcgg actcacgcat ggcgatcgta cgcgatcgac
     1441 acaacaatcg cgtaaacgac gggcagcaca tcattccgat ccccggcacc gcggtggcgc
     1501 gtcatcgatc tccggaacat gccaccaaag cgcttaatgc ttagtcaacc cccaacccca
     1561 acgccgggtt cggaggtact cgcgacgacc cggatccttc tgcgcgcgac gcgaagcatc
     1621 cgggtcgtcg gagtacctcc gaacccggcg tggcccgggt atgaaactcc ttcaaacgct
     1681 ggcttcacga ccgtcatgcc ccctaccatt gcaccaccga ggagagatca accgtatgtc
     1741 ccattcgcat ccgcatcctg ccaccgtcgg tcaactcaag gagaccgact atcgtccgat
     1801 gagcgtcaag gacgagcttc gcaaaaatct gatccgcaag ctcaaagccg gcgaacaatt
     1861 attccccggc atccttggct atcgcgacag cgtcatcccg caaatcgtca acggcatcct
     1921 gtccaagcac gacctgctgt tcctgggctt gcgcgggcag gccaagacgc gcatcctgcg
     1981 catgctgccg gacctgctcg atgagtggat gccggtgctg agcggcgtcg aaatcaatga
     2041 cgatccgctg agcccgatga ccaagaccgg tcagcgcatc gtcgccgagc aaggcgatga
     2101 cgcgaagatc gaatgggtgc atcgcagcga ccgctaccag gaaaaactgg cgacgccgga
     2161 tgtgactatc gccgatttga tcggcgagat cgatctggtg aagcacgccg aggggcgata
     2221 cctcagcgat gaatcgacga tgcactacgg catgatcccg cgttccaatc gcgggctgtt
     2281 cgcgatcaac gagctgccgg acctggcgcc gcgcattcaa gtcggcctgt tcaacgtgct
     2341 cgaagagcgc gacgtgcaga tccgcggcta tcccatccgc ctgaacctcg acgtctgcct
     2401 ggtcttcagc gccaaccccg aggactacac caaccgcggg cggatcgtca cgccgctgaa
     2461 ggaccgcatc ggctcggtca ttcgcacgca ttatccggag acggttcagg aaggcatcga
     2521 gatcgcccag cagaacgcgt gggtcgatcg cgacgcccaa cccagcggca gcggtgtgcg
     2581 cgttgatacg ccggcgttca tgaccgagat cgtggagcag gtcgtgcggc tggcgcgatc
     2641 gagcccgcat gtctcgcaag cctcgggcgt ctccgtgcgg acgagtattg ccaatttgga
     2701 gacggtgacg agcaacgccg agcgccgcgg gatcattacg ggtgaaaagc gcgtcgtgcc
     2761 gcgcatctgc gacttgaact atctcgtcgc ctcctgccgc gggaagatcg agatgacgct
     2821 ggccgaggaa gacggcgcgg aagacaagct ggtgcgctcg ctgatcggcg aagcggtgaa
     2881 gacggtcttc gccaagtatg ccgacaccga ttcatacgat cacatcagcg agcagttcaa
     2941 gagcaacctg acattccccg ccggggacga tctgtcggct gacgaattcg tcgccaacat
     3001 gaaagcggtc aacggcctgc acaagacggc ggtggcgctg tcgcgcgaaa tggagttaga
     3061 cggcggcgac cacgcgatcc tcgccagcgt cggggaattc ctgcttgagg gtttgtacgt
     3121 caacaaccgc ctgagcaagt acaacgccaa gggcaagacg ttcttcaagc ggtgagacgc
     3181 ggcgataaga cggtacaggt ccgacgataa gcggtcttga actgacccac gccgggttcg
     3241 gagcgtactc gcgacgaccc ggattcttct ggcgagcgaa aacgatccgg gtcgtcggag
     3301 tacctccgaa cccggcgtgg tcggtccttg tgcattcctg caaagcgtcg ggtcctctgc
     3361 atgtaaatgc gggaccccgg attcacgcta cgcgcgattc ttctgcgcca gttcgtactt
     3421 ctcctccgcc tccggtctcc tcacgtaaat cggcgtcagt gtgaacgggt cattgaattc
     3481 ccccgcggcg gcgaacgtgg cgccgatctc ggccaccgcg ctcgcgcggg cgcgccaacg
     3541 ttccggcggc gtgacgatca cacttccatc atccggcggg atgaactgct ggtgatacgg
     3601 gattccttcg cccagcaggt gcaccggccg cggggaacgc gcgagcatcg atgtcagatc
     3661 atcgagcttc gcgggctcgc gctcgagcca ttgtgagccg gcgcgttcga agcgggcggt
     3721 gaagatctga tcgcgcttgg cgtcgaggac gacgatcagg tgctgagcgt cggctggcgc
     3781 atttctcgcg agcacttgca ggctcggcgc cgcgacgagt ttcacgccgg tggccagcgc
     3841 catgactttg gcgagcgtga cgccgatgcg caggccggtg aacgacccgg gaccggcgga
     3901 gacgtagagg tgttcgacgt cgcgcggcga ccagccgcgc gagcgggtga ggcggtcgat
     3961 gatcggcacg atctcggctg cgtgcttgag gccgtgcggg aagacttctt cggcgacgac
     4021 ggcgtcgccg tcgatgaccg cgatcgatcc aagtcggccg gatgtttcga tggcgagccc
     4081 gcgcggcatg aacgcgattg tagcacgggc gtctcgcccg tgtctgacgg tgcacgacac
     4141 gggcgagacg cccgtgctac gaaaagattc agacgcggcg aggagtagcc gacgcgccaa
     4201 gcaccaagcc cgttgcgacc atcagcatcc ccaggccact gacgtacagc tgatatcgcg
     4261 catcgcgaca gagactgccc agaatacccg cgggcggtgc gtatggcgcg ggcgccggcg
     4321 ggggcgccac gctgaccagc atgaacggcg acatctcttc gcgcttcgaa ccgacgtgac
     4381 gcccgcgcag ttccagcacg ctggagcctt cgcggatgac cttcatctgc cgctgcgcga
     4441 gccgctcggc caggctcgcg tcgccgccga cgttcttcag gaattcgctg ttgacggaaa
     4501 tctgcgtgtc cacgtacgcg aggtcgcgct tgagcttatc gcgctcggcg gcgaggcgct
     4561 tgttcgcctc cgcctgcggc accagcacgc acgcggcgat gacgcccagg cccaggcaca
     4621 tcagcgggat cgaccaggcg ctggaccagg tgcggggagg aagttggctt ctttggtcgt
     4681 ccatgacggt ctgatcgtga atacgtgatt acgtgattac gcgacaagat cggttcttta
     4741 tcacgtaatc acgtattcac tcatcacggg cttacttgtt ccagaactgc gcgccgtact
     4801 gcgcgtgttc gcccagttgc tcttcgatgc ggagcaactg gttgtacttc gcgacgcggt
     4861 cgcttcgcgc gggcgcgccg gtcttgatct gaccgcagtt ggttgcgact gcgatgtccg
     4921 caatcgtcgc atcttcggtc tcgccgctgc ggtggctcag gaccgcgctg tagccattgc
     4981 ggatcgccat gttcaccgtg gcgaacgttt cgctgagcgt gccgatctgg ttcaccttca
     5041 ccagcacgct gttggcggtg ccggtcttga tgcccttttc caggaacttc acgttggtga
     5101 cgaacagatc gtcgcccacc agctggattt tcttgcccag gcggtcggtc agcttcttcc
     5161 agccatccca gtcgttctcg gccaggccat cttccagcga gcggatcgga tatttctcga
     5221 cccacttcgc ccagtaatcg accatcgcct cgggggccag cactttatcc ggcgcgctct
     5281 tgaagaactt gtaacccttc ttttccttgt cccacaattc cgacgcggcc ggatcgagcg
     5341 cgacgaacac ctgcttgccg aattcatacc ccgcggcggt gacggcttcg ccgatcacct
     5401 tcagcgcgtc ttcgttgtcc ttcaggtccg gggcgaaccc gccctcgtcg ccgacggcgg
     5461 tgctcatctt gttcttgtgc agaacctttt tgagcgcgtg ataaatttcg acgccggcgc
     5521 gaaggccgtc gctgaatgac tcgaaacccc acggctggat catgaattcc tggaagtcga
     5581 cgttgttgtc cgcgtgcttc ccgccgttga ggatgttcat catcggcacc ggcagcgtct
     5641 tggcggccgg gccaccgagg taacgataca gcggcagccc gctgctcttg gcggcggcct
     5701 tggcgacggc cagcgacaca cccagcatcg cgttggcgcc cagcttcgcc ttgttcggcg
     5761 tgccgtccat ctcgatcatc agttgatcga tggcttcctg gtcgcgggcg tcgaggccga
     5821 tcagctcggg ggcgatcttg ctgttgacgt tgtcgacggc cttgaggacg cccttgccga
     5881 cgtagacctt cttgtcgtcg tcgcgcagct cgacggcttc gtactcaccg gtgctcgccc
     5941 cgctggggac tgctgcccga cccatcgtgc cgtcttcgag gatcacgtcc acttcgatcg
     6001 tgggattgcc gcgggaatcg aggatctgcc ggccctgaat gaattcgatg ctgacgctca
     6061 taatgtttgc caaaccagcc ggcgtggaga gacgcacgca cgccaccggc ggaggcttgg
     6121 ccctttgggt tatcggacgg atggcttgcc agactgcaac cgaagcagca ctgggcatgc
     6181 gcggtgaagc gcagcaccgg agtttatgcg caggggggta gctgtcaaac gggaagaacg
     6241 atcgatcgca cagcgcttgc gcagcggtcg ccccgcaggg gcatggtctt ctcgttcgag
     6301 cgcaaaaaca cgtgcccctt cggggctacc gctacgcggg ggaatgaaga atccacggtt
     6361 gtcgttatgc caggatggct tttgaaatgc gggcggcgct ggcgcgggcg gcagggtcca
     6421 gcagcagctg caccgccgcc tgacccagtt gctgttccac ccggtgcacc tgctccgatc
     6481 gcatcacctg cttgatgcgc acgccgatcg agaatcgatc cgcctccagc cgccgcgagt
     6541 gggcggccac gcaaatgagc cagatcggtt caccctggaa cgattgcagc tggatgatga
     6601 cgcgctggtc cttggcgaac tttgtgctcg tgcagaatcc gatgccgctg cgcgagatat
     6661 cgcggatgcg cacgcgcgag attcggcgct tgccgtcgtc gcagatgctg acgagattcg
     6721 cctcgccggc catgccgaca cgcggctcga tacgctgatc gcgatcgccc ttgcggtccg
     6781 accgcagggc ttcgacgatg tcgttgaaca tttcggccga taatcgcata cgtctgacgc
     6841 ggcgtgacac cccgtccttt atagaatcgg gcaaacagag cctctcgctt aaccccggcc
     6901 gcgacacgaa atgttgcgtc ggccatggtt aaaggcacca gtgccccctc cccaccgcct
     6961 gcggccgcag aattacaccg ccccccgcgc gcgactgccc ttcggcgagc cgacgaatcg
     7021 tatgtctcac cgggtcgtag ctaacaccat gaaggggtgt gttatgcaga atcgcacgcg
     7081 ggatgatcgg accctgcgcc ttttttctgc gcgcgcccac cgggctaacc gcttcagcgg
     7141 ttcgcggagc acctcggcgc ctcgtgcaca gatcgcgcgc atcggcgtca atcacgtcac
     7201 gatatcaacg cggaagtcgc cgaggttcac gcccttgtcg ctgaagtacg cggacagctg
     7261 acccgcatcc tgcagctgcg tccgcgacgg cgccacacgg atgccgccga ccgtcgcggt
     7321 cagcgacgtg aacgccaccg cggccacgcc ttcgggcgtg ccgttgttgt tggtgttcac
     7381 atcgcccagg gcggcggtgg tgatcgtgtg cgcgatgacc gtcgatcccg cgaatgcccc
     7441 ggcggtgcgg gcggtcaagc gcagcgaacg gatcgcgtgc gagccgatgt tgcccggcgt
     7501 caccgcgtcg atcgtcgtta cgccgccgtc aacgccggcg gtgatcgtgg acccggcgag
     7561 cgagctggcg atcagcgctg tgagatcgcc ggcggtggcg atcgtgctgc cggtgatcgc
     7621 gttgccgacg atcatcgcgc gaagcgatgg cgtcgccgac gccgggccgt tcatcgtaat
     7681 cgtcgagttg gcaaccgaac cggtgacgcg gatgacgttg gccgaagcgg cggtgattgc
     7741 cgccccgccg ttcacgtcac cgataacgag catcgtcccg atgccgccgg ccgacagatt
     7801 cgacgcgaag ctgccgccgc gatcgacgat gacattgagc aacccggcaa gcgtgccgct
     7861 ccacgcgctg gtgatcgacc cggcgacgat cgtgttcgcc cggccgttga tcgcccagct
     7921 gtcgccaccg atggcgccga caacttgagc gacgcccagg gtcggcaagg ccgatccgct
     7981 gccggacagg tttagcgccg cgttgaagcc gccgagcgaa atcagcgttg cgatcaacgg
     8041 cgcagagatc gaactgttgt ttgcagtgtc gctccaggat gcaacgcgca tcacccggat
     8101 cggaacagaa ctgttgagcg acgagtccgc catcgccccg ccgacgatca cgacaccggc
     8161 gggcgtgccg gcgttg
//
LOCUS       DATIMH010000256         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1789947, whole genome shotgun sequence.
ACCESSION   DATIMH010000256 DATIMH010000000
VERSION     DATIMH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789947"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1953)
                     /locus_tag="VLI90_04340"
     CDS             complement(49..1953)
                     /locus_tag="VLI90_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSV13462.1"
                     /translation="MSVPRDNPELLKAQYRAFSRQLPLMYFILLTNTWALAVSHYAVA
                     PHWLTVYCPAGFTALCGIRVIGWWRSRSVVPTAEIAFRALTQTNRLTSILTIAFSAWA
                     LALCPYGDLLARAHVAFYMAITVIACIFSLMHLRPAAFTVAICANIVFIAFFGSSGNI
                     VFMAMAVNALLVTIAMMFILETNYRNFARMVDGRTSLKALSNENFRLANLDSLTRLPN
                     RRQFFAQLDQEFEAAQTQGYRLAVGVIDLDGFKPVNDIYGHAVGDKLLMAVGERLTEA
                     CGGVHVARLGGDEFALIVKQVNDDELLKQGEKICATLRKTFELKEATVQISSSVGFTV
                     FPELASNATDLFEQADYALYHSKRTSRGNAMLFSTAHEAEIHRNAKIERALRAADLGN
                     ELSAVFQPVVDARTEKTVGFEALARWNSPTLGHVPPDQFIPVAERAGIVNRLTRVLLE
                     KALTAATRWPDDIRLSFNLSNHDISSPEGVIRIIGIILKSGVDPKRIDFEITETAIMR
                     DFEQARSAINSLQTFGCGISLDDFGTGYSSLSQLHALPLTKIKIDRSFVTQLHERPAS
                     HKIVKSLIALSRDMGLGCVIEGVETSEELAAVLHLGGFEIQGYYYSRPLPELELAAYL
                     DAAVAVAAIA"
     gene            complement(2193..>2701)
                     /locus_tag="VLI90_04345"
     CDS             complement(2193..>2701)
                     /locus_tag="VLI90_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13463.1"
                     /translation="AGTAKTTLYKLFGSKTNLVHAVLESEGKQWREWFITSIETGGGD
                     AQAKLTRIFPALKQWFAEERFYGCPFINAVGEHDKDAKQFRAIALRHKKVVLSHIEKL
                     AGEMGAGEPEMLAHQLALLIDGAIVAAMVTRDPTVADTAGLAAGNLFGPSKAKKPKRA
                     SVAQLVAV"
BASE COUNT          493 a          798 c          866 g          544 t
ORIGIN      
        1 gccatcacgg gacgagctgc gcagccgcaa ctcgttcgct tcggttcttc acgcgattgc
       61 cgcgaccgca acagcggcgt caagatacgc cgcaagttcc agctctggta acggccgcga
      121 atagtaatag ccctggatct cgaagccgcc gaggtgcagc accgccgcca gttcttctga
      181 tgtttcgacg ccttcgatca cgcagccgag gcccatgtcc cggctcagcg cgatcagcga
      241 cttgacgatc ttgtggctcg ccggccgttc gtgcaattgc gtcacgaagc tgcggtcgat
      301 cttgattttg gtcagcggca gtgcgtggag ctggctcagg ctcgaatagc cggtgccgaa
      361 atcatccagc gagatgccgc agccgaacgt ctgcaggctg ttgatcgccg agcgcgcctg
      421 ttcgaaatcg cgcatgattg cggtctcggt gatttcgaaa tcgatgcgtt tgggatcgac
      481 gccgctcttg aggatgatcc cgatgatccg gatcacgccc tcgggcgaac tgatgtcgtg
      541 gttcgagaga ttgaacgaca gcctgatgtc gtcgggccaa cgggtggccg cggtcagcgc
      601 cttttccagg agaacgcgcg tcaggcggtt gacgatgccg gcgcgctccg ccaccggaat
      661 gaattggtcc ggcggtacat ggccaagcgt cgggctgttc cagcgcgcca gcgcctcgaa
      721 tccaaccgtc ttttccgtcc gggcatcgac gaccggctgg aacacggcgg acagttcgtt
      781 gccgaggtcg gccgcgcgca gggcgcgttc gatcttggcg ttgcggtgga tttcggcctc
      841 gtgtgcggtc gagaacagca tggcgttgcc gcggctggtt cgcttgctgt gatagagcgc
      901 gtagtcggcc tgctcgaaca ggtcggtcgc gttggaagcc agttcgggga agacggtgaa
      961 gccgaccgag ctcgatatct gcacggtcgc ttccttcaat tcgaaggtct tgcgcagggt
     1021 cgcgcagatt ttctcaccct gcttcaggag ttcgtcgtca ttgacctgtt tgacgatcag
     1081 cgcgaattcg tcgccgccga gacgcgcgac gtgaacgccg ccacaggcct ccgtcagtcg
     1141 ctcgccgacg gccatcaaca gcttgtcgcc gacggcgtga ccgtagatgt cgttgaccgg
     1201 cttgaaccca tcgaggtcga tgacccccac ggcgagccgg tagccttgcg tttgggctgc
     1261 ttcgaattcc tggtcgagct gcgcgaagaa ctggcgccgg ttcggcagtc gggtaaggct
     1321 gtcgaggttc gcaaggcgga agttttcgtt gctcagggcc ttcaggctgg tccgtccgtc
     1381 gaccatccgg gcgaaattcc ggtagttggt ctcgaggatg aacatcatgg cgatggtgac
     1441 gagcagcgca ttgaccgcca tcgccatgaa cacgatgttg cccgacgatc cgaagaaggc
     1501 gatgaagacg atgttggcgc agattgcgac cgtgaaggcg gccggacgca ggtgcatcag
     1561 gctgaagatg caggcgatca ccgtgatcgc catgtagaac gcgacatggg cgcgggcgag
     1621 caggtcgccg tagggacaaa gcgcgagcgc ccacgcgctg aatgcaatgg tgaggatgct
     1681 ggtgagccgg ttggtctggg tcagcgcgcg aaaggcgatc tccgcggtcg gcaccaccga
     1741 tcgcgatcgc caccagccga tcacgcgaat cccgcacagc gcggtgaagc ctgcggggca
     1801 gtaaaccgtc agccagtgag gggcgaccgc gtaatgcgaa accgccagcg cccaggtgtt
     1861 ggtgagcagg atgaaataca tcagcggcag ctgccgcgaa aacgcccgat actgcgcctt
     1921 cagcagttcg ggattgtccc gcgggaccga catgaactgg cgaatcttct taaaaatgtc
     1981 ttgggtattc atgacgctgt cccggaacca ccaacagatc cggggctacc atcgcgcttt
     2041 ttcgacccat gatttcttaa gccgcgggat agtagaaata ctggttgcga ccagcaggtc
     2101 atgtcgagtt ctgttaacct tgccggatcg ttgctccgag catggcggcg gaccgcatgc
     2161 gtgaacgcat ccgctgctcc tgtcggtggc agtcagacgg cgacgagttg cgcgacgctc
     2221 gctcgcttcg gcttctttgc cttggaaggc ccgaacaaat tgcccgctgc gagccctgcg
     2281 gtatcggcaa cagtgggatc gcgggtcacc atggcggcga cgatggcgcc gtcgatcaac
     2341 aatgcgagct gatgcgccag catttccggt tcgcctgcgc ccatctcacc ggcgagtttt
     2401 tcgatatgcg acagcaccac cttcttgtgc ctcagcgcaa tggcgcgaaa ctgcttggca
     2461 tccttgtcgt gctcgccgac ggcgttgatg aaagggcagc cgtagaaacg ctcttcggcg
     2521 aaccattgtt tcagcgccgg aaagatccgc gtcagcttag cctgcgcgtc gccgccaccg
     2581 gtttcgatcg aggtgatgaa ccattcgcgc cactgcttgc cttcgctttc cagcacggca
     2641 tggacgaggt tggtcttcga tccgaacaat ttgtagagcg tggtcttcgc ggtgccggct
     2701 t
//
LOCUS       DATIMH010000257         1514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5255793, whole genome shotgun sequence.
ACCESSION   DATIMH010000257 DATIMH010000000
VERSION     DATIMH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5255793"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VLI90_04350"
     CDS             complement(<1..778)
                     /locus_tag="VLI90_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HSV13464.1"
                     /translation="MRIRLLSILLAIATCAAAGAKTITVAADGGGDFKTIQEAIAAVP
                     DKSAERTVIHIRAGKYEGQIFIPQAKTKIAFEGDGVDRTFITYHFNTNEPNPPGVGRN
                     YWGTAVVVLSDDFTAHNLTFENESGDHGQALALRIDGDRAVVAGCHLVGWQDTLMVNN
                     GRQYFADCYIAGRVDFIYGSATAVFDRCQIHSRNGGHVTAASTPQDHPYGLVFLNCKL
                     TGDATPWDPATTNPASTVKPRVTPIADLGRPWRPYASVAYI"
     gene            complement(840..>1514)
                     /locus_tag="VLI90_04355"
     CDS             complement(840..>1514)
                     /locus_tag="VLI90_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HSV13465.1"
                     /translation="TDTAGWAKGFVACLKDDVQCTNLSRGGRSSKSFIAEGLWKKCLD
                     LKPDYVLIQFGHNDQPGHPGDRETDPQTTYKQFMTQYVDDARAAGIKPVLVTSMSRRQ
                     WGPDGKIHSTLVPYVEVVKEIAAAKHVPLIDLHERSIELYEKLGKDGCNALSPLKPAT
                     TKPTKDAEAVADNKTAGMVYDGTHLNEKGSAIVGPIVAAELAKVVPELAPHIHTGEPT
                     TQLRDR"
BASE COUNT          241 a          476 c          495 g          302 t
ORIGIN      
        1 tgatgtacgc gacgctggcg tagggccgcc aggggcggcc aaggtcggca atcggcgtga
       61 cgcgcggctt caccgtgctc gccgggttcg tcgttgccgg gtcccacggc gtcgcgtcac
      121 cggtgagttt gcagttcagg aacaccagcc catacgggtg atcctgcggc gtgctggcgg
      181 cggtcacgtg cccgccgttg cggctgtgga tctggcagcg atcaaacacc gcggtggccg
      241 atccgtagat gaagtccacg cgaccggcga tgtagcagtc ggcgaaatac tgccggccgt
      301 tgttcaccat cagcgtgtcc tgccagccga ccagatgaca tccggcgacg acggcgcgat
      361 cgccgtcgat gcgcaacgcc agcgcctgcc catgatcgcc ggattcgttc tcaaacgtca
      421 ggttgtgcgc ggtgaagtcg tcgctgagca cgaccaccgc ggtgccccag taattccgcc
      481 cgacacccgg cgggttgggc tcgttggtgt tgaagtgata ggtgatgaac gtgcggtcca
      541 cgccgtcgcc ttcgaaggcg atcttcgtct tggcctgcgg aatgaaaatc tgcccttcat
      601 acttgccggc gcggatgtgg atgacggttc gctcggccga cttgtcgggc acggcggcga
      661 ttgcctcctg gatcgtcttg aaatcaccgc cgccatcggc ggcgacggtg atggtcttcg
      721 cgcccgccgc ggcacaagtg gcgatggcca gcaagatcga caggagtcgg atgcgcatta
      781 gataccctcg aaagacagat gttttctaag agcgatgacg aacggtcgcg cgttgtgtat
      841 cagcgatcgc gaagttgcgt ggtcggctcg cccgtgtgga tatgcggcgc caactccggc
      901 acgaccttcg ccaattccgc ggcgacgatg ggaccgacga tcgctgaacc cttctcgttc
      961 aaatgcgtgc catcataaac catccccgcg gtcttattgt cggccaccgc ctcggcatcc
     1021 ttggtgggtt tggtcgtcgc gggcttcagc ggcgacagcg cgttgcagcc gtccttcccg
     1081 agcttttcgt acagctcgat cgaccgctcg tgcaggtcga tcagcggcac gtgcttagcc
     1141 gcggcgatct ccttcaccac ctcgacatat ggcaccagcg tcgagtgaat cttgccgtcg
     1201 gggccccatt gccggcggct catcgacgtg acgagcaccg gcttgatgcc cgccgcgcgg
     1261 gcgtcgtcca cgtattgcgt catgaactgc ttgtacgtcg tctgcggatc ggtctcgcgg
     1321 tcgccggggt ggccgggctg gtcgttgtgg ccgaactgga tgaggacata gtcgggcttg
     1381 aggtccaaac acttcttcca caacccctcg gcgatgaagc tcttggagct gcgcccgccg
     1441 cgggacaggt tcgtgcactg gacgtcgtcc ttcaggcagg cgacgaagcc cttcgcccag
     1501 ccggcggtgt cggt
//
LOCUS       DATIMH010000258         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3233732, whole genome shotgun sequence.
ACCESSION   DATIMH010000258 DATIMH010000000
VERSION     DATIMH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3233732"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2298)
                     /locus_tag="VLI90_04360"
     CDS             complement(<1..2298)
                     /locus_tag="VLI90_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13466.1"
                     /translation="MDVENALGLTMSGEAPPASVRQRRLRQVNLQLVASGLPVTSDGD
                     GEFSAVAKGVLDSFRMQARLLGEHRCPVDRRIESFLQAHFADETRGQAPLRLPDRTVI
                     LGQHGIARELSLPAHGDQFANDLVTSYRVRNGVLHNPKNDRRTTKGTFHVAEGGLPVP
                     FDKKAVPKAVFAQLFRAAMNSPKEMLEIPFTHGAPRPANSFVSLLLRPLVCPAVPGVC
                     PRRTMEIRFFAPGSLVSNLDFVESIFGNAGDPFLPQNDAALDVEHWTGHTGCVILAPH
                     LVTLTKKELGLPHFNEASERQRRDGMCWRDEKEKYNDGSAFKITCRTDAGVIVTIVAD
                     NYFGYCKKEVKTQISYAANLYGNVEEEHAGGAIAFVSYSFGDEYQTDSSKVNNRTFDD
                     VLRDYSSLMDAKPEGYGVDRTFRDLIYIPEDARASVKRQQVWWTRGGKEQAVPLLPGK
                     IYMTPSGFKLRMEKHPGAPSWRIIGTVGQGVFCHKPCTVSGGGKSEISKGIRDYMLYG
                     PIFMSDVERDLDLVDAIFHRDYSDRWRKESHIKPDYAKKRSRPILSPERSLGSVIKLL
                     TPSADYTDGYNRWLASIPNYVYPIVFIIKRFYRPEWNGNWREHFGVDIVNGFPGHELK
                     YEDRKLVGTYLRVGLMPPALWRTYKVRQDFAAATKIQTEDDISASVVVPARQLAGATP
                     ADADKSLKFVTNCEFRLFQRPDDAIHRGLDKQTESDMSRADNFISNFEPLTDNDARAM
                     VERVTEFDQFTEPMQRLLRRAAESGE"
BASE COUNT          401 a          786 c          761 g          486 t
ORIGIN      
        1 ctcgccgctc tcggcggcgc ggcgcagcag gcgctgcatc ggctcggtga attgatcgaa
       61 ctccgtcacg cgctcgacca tcgcgcgggc atcgttgtcc gtcagcggct cgaagttcga
      121 gatgaaattg tccgctcgcg acatgtccga ttcggtctgc ttatccagcc cgcgatgaat
      181 cgcatcatcc ggccgctgga acaggcggaa ttcgcagttc gtcacgaact tcaagctctt
      241 gtcggcatcc gccggcgtcg ctcccgccag ttgccgcgcc ggcaccacga ccgatgccga
      301 gatgtcgtct tcggtttgaa tcttcgtcgc cgccgcaaaa tcctggcgga ccttgtacgt
      361 ccgccacagc gccggcggca tcaggccgac gcgcaggtac gtgccgacca gcttgcggtc
      421 ttcgtacttc agctcgtgac cggggaagcc gttgacgata tcgacgccga aatgctcgcg
      481 ccagttgccg ttccattccg gccgataaaa tcgcttgatg atgaagacga tcgggtaaac
      541 gtaattggga atcgacgcca gccagcggtt gtaaccatcc gtgtaatccg ccgacggtgt
      601 gagcagcttg atcacgctgc cgagcgagcg ctcggggctg aggatcggcc ggctgcgctt
      661 cttcgcgtag tccggcttga tgtgcgattc ctttcgccag cggtccgaat aatcgcggtg
      721 aaagatcgca tcgaccaggt ccaggtcgcg ctcgacgtcc gacatgaaga tcgggccgta
      781 cagcatgtaa tcgcgaatgc ccttgctgat ttcgctcttg ccgccgccgc tgacggtgca
      841 cggcttgtgg cagaacacgc cctgccccac cgtcccgatg atgcgccagc tgggcgcgcc
      901 cgggtgcttt tccatgcgca gcttaaaccc gctgggcgtc atgtagatct tgcccggcag
      961 cagcgggacc gcctgctctt tgccgccgcg ggtccaccag acttgctggc gtttcacgct
     1021 ggcgcgcgcg tcttcgggga tgtagatcag gtcgcggaac gtgcgatcga cgccgtagcc
     1081 ctcgggcttg gcgtccatca gcgagctgta atcgcgcagc acgtcgtcga acgtgcggtt
     1141 gttcaccttg ctcgagtcgg tctgatattc atcgccgaag ctgtagctga cgaacgcgat
     1201 cgccccgccg gcgtgttctt cttcaacgtt gccgtacagg ttcgccgcgt aactgatctg
     1261 cgtcttcact tcctttttgc agtaaccgaa gtagttatcg gccacgatcg tgacgatgac
     1321 gccggcgtcg gtgcggcagg tgatcttgaa cgcgctgccg tcgttgtatt tctccttctc
     1381 gtcgcgccag cacatgccgt cgcggcgttg ccgctcgctg gcttcattga agtgcggcaa
     1441 tcccagttcc ttcttcgtca gcgtgaccag gtgcggcgcg aggatgacgc aaccggtgtg
     1501 gccggtccaa tgctccacgt ccagcgcggc gtcgttctgc ggcaggaatg gatcgccggc
     1561 gttgccgaag atcgattcaa cgaagtccag attgctgaca aggctgccgg gcgcgaagaa
     1621 gcggatctcc atcgtccgcc gcgggcaaac cccgggcacc gccgggcaaa ccagcgggcg
     1681 aagcagcagc gacacgaacg agttagccgg cctgggcgcg ccgtgcgtga acgggatctc
     1741 cagcatctcc ttcggtgagt tcatcgcggc gcgaaacaat tgcgcgaata ccgccttcgg
     1801 caccgccttt ttatcaaacg gcaccggcag cccgccttcg gccacgtgaa acgtgccctt
     1861 ggtggttcgg cggtcgttct tcgggttgtg caacacgccg ttgcgtacgc ggtaggacgt
     1921 caccagatcg ttggcgaatt gatccccgtg cgcgggcagc gacagctcgc gcgcgatgcc
     1981 gtgctggccg aggatgaccg tgcgatccgg caaccgcagc ggcgcttggc cgcgcgtctc
     2041 gtcggcgaag tgcgcttgca ggaatgattc gatgcgccga tcgaccgggc agcgatgctc
     2101 gcccagcagg cgggcctgca tgcggaagct gtcgagcacg cccttggcga cggcgctgaa
     2161 ttccccgtcg ccgtcggatg tgacgggcaa tccgctggcg accaactgca ggttcacctg
     2221 tcgcaaccgc cgctgccgca cgctcgccgg cggcgcttcg ccggacatcg tcaaccccaa
     2281 cgcattttca acgtccattg ccaaaacgtc ctctctgggt gcggttcatc cacggtggga
     2341 gcgggcgcgc tcgatgtcgg caacgcgcat tatctaagaa tgacgccgcg tgtcgatgat
     2401 agcgcgcgcg gccgccgctt gcggcttagc atct
//
LOCUS       DATIMH010000259         5996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4967232, whole genome shotgun sequence.
ACCESSION   DATIMH010000259 DATIMH010000000
VERSION     DATIMH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4967232"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1292
                     /locus_tag="VLI90_04365"
     CDS             144..1292
                     /locus_tag="VLI90_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HSV13467.1"
                     /translation="MMKTAVNRAKAYLKVRHQNVRAAMKELKLDGLLLTHPPDLAYLT
                     NFTGDDSIGLITEKDFFLATDFRYQEQADLEAGWLKQHIREAKMSDTLAKMLAEAKVK
                     RIGFEANFTTVGQVDALETALKGKDGAGNGSPVKVELVPLDNVMVNIRKVKDDHEIDL
                     VRKSVAVAEEAFEAIRSEIKVGQTENYLAGLLVFELRSRGASDSSFPCIVAAGANSSL
                     PHYRPQETLVARDQPLLFDWGALNKGYCSDLTRTLMIGRVSQRIKKIYQVVLEAQMEA
                     IKFLRPGVTTLQADRVARDVIEKAGFGKEFGHGLGHGIGRDIHELPTMRKTGGEEELR
                     PGMIVTVEPGIYLPGEGGVRIEDDCLITHSGCEVLSTLDKSFEGCHME"
     gene            1314..1853
                     /gene="accB"
                     /locus_tag="VLI90_04370"
     CDS             1314..1853
                     /gene="accB"
                     /locus_tag="VLI90_04370"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HSV13468.1"
                     /translation="MSKKKANNRSTAAPPAKSTGPVSPMNVGLLEQIVKLMAANDLNT
                     VDLRDGNQRVVLKRGAAAYMTAAPVTYTAPSPAPTFAGAASSPASPMAATPASDEANL
                     VPIKSPMVGTFYSAGSPDAKPFVQVGSTVDEDTDVCIIEAMKVFNNIKAETRGTIAKV
                     LISNGQTVEFGQTLFLVKP"
     gene            1850..2770
                     /locus_tag="VLI90_04375"
     CDS             1850..2770
                     /locus_tag="VLI90_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HSV13469.1"
                     /translation="MSTTSTPTRRPLAFATEDGVLAEVARLRRHGYQKTGNWSLPQIA
                     WHLMIPIDAYLNPPASPDIQPTPEQAKMKAGFVDYIVANGKLPPHATTAPPSWTPPAD
                     AGDADVERLVIGMNKLKNYPHAMVEMSVIGPVPIAECRRVHLVHAAHHLSFLQPKNAR
                     RDVLRFKSPDQAIADVRALKKGHKQAGDWTLPQACWHLNVALGNTMRPGPHAESAPMT
                     DELRRRLQGILNTGQLPQGVQAPATAAPPADAPESSIDTFIATLERWESYRGDFAPHR
                     IFGKISDDEMCQLAMIHCAHHLSFFVPTGE"
     gene            2783..4129
                     /gene="accC"
                     /locus_tag="VLI90_04380"
     CDS             2783..4129
                     /gene="accC"
                     /locus_tag="VLI90_04380"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HSV13470.1"
                     /translation="MFSKILIANRGEIALRIIRACKELGIQTAVVYSTADKDAAYLQL
                     ADQAICIGNAPPAESYLNIPRIISAAEIADVQAIHPGYGFLAENSHFAEVCRSCKIEF
                     IGPPVKAMAAVGDKVACKKLAKKAKVPTVPGSDGAVDDEKIALRLASEIGYPVIIKAA
                     AGGGGRGMRVAHNDVSLRAGLKQAQAEAENAFKDSTVYIEKYVEFGRHVEVQILADSH
                     GNAVHLWERDCSMQRRHQKLVEESPSPVLRQEVRERLCDSAVRLVKAAGYTNAGTVEF
                     LVDKDQNFYILEVNARIQVEHPVTEQVTGIDLIKEQIRIAAGEPLSFKQTDVRQIGHA
                     IECRINAEDPARNFAPSPGLIIELRVPGGPGVRLDSHAYAGYRIPPNYDSMIGKLIVH
                     RNTRAEAIATMKRALGEFHISPIKTTIPLHLQIMDNEYFRSGDVDTGFVERVLLQK"
     gene            complement(4491..4649)
                     /locus_tag="VLI90_04385"
     CDS             complement(4491..4649)
                     /locus_tag="VLI90_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13471.1"
                     /translation="MTQARHPISGFEKVDWLGHWTNVRRPPRRRFTIALRHHFNQRGS
                     ERANTRIT"
     gene            complement(4753..5376)
                     /locus_tag="VLI90_04390"
     CDS             complement(4753..5376)
                     /locus_tag="VLI90_04390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSV13472.1"
                     /translation="MTKVLLIRHGATVLTLEDRFAGSTDVQLSDDGRSQAQRLGLRLA
                     ATKIAAVYCSDMHRAMDTAAAVARPHGLTPIVRAGLREIDHGQWEGMVHKAVEQQYAA
                     EYQAWEANPFLVAPPGGETGLTVLARSLPELRRIVLDHPDQTVAVVSHKATNRLLLCS
                     IMGIDPQMYRARISQDTACLNVFEFCESMSGRVTLLNDVSHYSTSEA"
     gene            5709..>5996
                     /locus_tag="VLI90_04395"
     CDS             5709..>5996
                     /locus_tag="VLI90_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13473.1"
                     /translation="MRLSLRATLLAALFLFCLSPIIARADLPPMPETAHPIDSGATAW
                     MLTSTALVLLMVPGLAMFYGGLVRTKNVLGTMMHSFSAMAVVGVLWVVIGYA"
BASE COUNT         1178 a         1923 c         1827 g         1068 t
ORIGIN      
        1 attacgactt tccgttggcg ctgccagccc cgcggttggt tgacagtccc accggctgtc
       61 cgctaacgtt tcgcctcctt agaacccggt gccgcgcagg cgcattggtg cggtgccgcc
      121 gaaggttttt ccggatcagc agcatgatga aaacggcagt gaatcgagcc aaggcgtatc
      181 tcaaagtccg ccaccagaac gtgcgggcgg cgatgaagga actgaagctc gacggcctgc
      241 tgcttaccca tccgcccgac ctcgcctacc tcaccaactt caccggcgac gactcgatcg
      301 gtctgatcac cgaaaaggat tttttcctcg ccaccgactt tcgctatcag gaacaggcgg
      361 acctggaagc gggctggctg aagcagcaca tccgcgaagc gaagatgtcc gacacgctgg
      421 ccaagatgct ggccgaggcg aaggtgaagc gcatcggctt cgaagcgaac ttcacgaccg
      481 tcggtcaggt cgatgcgctg gagacagcgc tgaagggcaa ggacggcgcg ggcaacggat
      541 cgccggtgaa ggtcgagctg gtgccgctcg acaacgtgat ggtcaacatc cgcaaggtga
      601 aggacgatca cgaaatcgat ctggtgcgca agtccgtcgc cgtggccgaa gaggcgttcg
      661 aagcgatccg cagcgaaatc aaagttggcc aaactgaaaa ttacctcgct gggctgctgg
      721 tgttcgagct gcgttcgcgc ggcgccagtg acagcagttt tccctgcatc gtcgcggcgg
      781 gcgcgaatag ctcgctgccg cactatcgcc cgcaggagac gctagtcgcg cgcgatcaac
      841 cgctgctgtt cgactggggc gcgctgaaca agggatattg cagcgacctg acgcgcacgc
      901 tcatgatcgg ccgggtcagc cagcgcatca agaagatcta tcaggtggtg ctcgaagcgc
      961 agatggaggc gatcaagttc cttcgccccg gcgtgacgac gctgcaggcg gatcgtgtcg
     1021 cgcgcgacgt gatcgaaaag gccggcttcg ggaaggaatt cggccacggg ctcggtcacg
     1081 gcatcggccg cgatattcac gaactgccga cgatgcgcaa gaccggtggc gaagaagagc
     1141 ttcgccccgg catgatcgtc accgtcgagc cgggcatcta tctgcccggc gaaggcggtg
     1201 tgcggatcga ggacgactgc ctcatcacgc actccggctg cgaagtgctt tcgacgctcg
     1261 acaagagctt cgaaggctgc cacatggaat aacagggaat tgacgaggtt tgattgagca
     1321 agaaaaaggc gaacaacaga tcaaccgccg cgccgccggc gaaatccacc ggtccggtta
     1381 gcccgatgaa cgtcgggctg ctggaacaga tcgtcaagct gatggcggcg aacgatctga
     1441 acacggttga cctgcgcgac ggcaatcagc gcgtcgtcct gaagcgcggc gctgccgcgt
     1501 acatgaccgc ggctcccgtg acttacaccg cgccgtctcc cgctccgaca tttgccggcg
     1561 ccgcttcatc gcctgcttca ccgatggctg caacgccggc ctcggatgaa gcgaacctcg
     1621 tccccatcaa gagcccgatg gtgggcacct tctactccgc cgggtcaccc gacgccaagc
     1681 cgttcgttca agtcggttcc accgtcgatg aggacaccga cgtctgcatc atcgaagcga
     1741 tgaaggtctt caacaacatc aaggccgaga cgcgcgggac gatcgccaag gtgctgatca
     1801 gcaacggcca aacggttgaa ttcgggcaga ccctcttcct ggtcaaacca tgagcacgac
     1861 gagcactccc acccgacggc cgctggcgtt tgcgacggaa gacggtgtgc tggccgaggt
     1921 cgcgcgcctg cgccgccacg gttatcagaa gaccggaaac tggtcgctgc cgcagatcgc
     1981 ctggcacctg atgatcccga tcgacgcgta tctgaatccg ccggcgtcgc ccgacatcca
     2041 accgacgccc gagcaggcga agatgaaggc tggcttcgtc gattacatcg tcgccaacgg
     2101 aaaactcccg ccgcatgcga cgaccgcgcc accgagctgg acgcccccgg ccgacgccgg
     2161 cgatgccgac gttgagcgcc tcgtcatcgg gatgaacaag ctcaaaaatt atccgcacgc
     2221 gatggtcgag atgagcgtga tcgggcccgt gccgattgcc gaatgccgcc gggtgcacct
     2281 ggtgcacgcc gcccatcacc tgtcgttcct gcaaccgaaa aacgcccggc gtgacgtgct
     2341 gcgattcaaa tcgcccgacc aagcgatcgc cgacgttcgt gcgttgaaga agggccacaa
     2401 gcaggcgggt gattggacgc tgccgcaggc gtgctggcat ctgaatgtcg cgctcggcaa
     2461 cacgatgcga ccgggaccgc acgcggaaag cgcgccgatg acggacgagc ttcgccggcg
     2521 attgcaggga attctgaaca cgggccagct gccgcagggc gttcaagctc cggcgaccgc
     2581 cgcgccgccg gccgatgcgc ccgagtcatc gatcgacacg ttcatcgcta cgctcgagcg
     2641 atgggagtca taccgcggcg acttcgcgcc gcatcgcata ttcggcaaga tcagcgacga
     2701 cgagatgtgc caattggcga tgattcattg tgcgcaccac ctgagcttct ttgtccccac
     2761 gggcgagtga gaccactacc gcatgttttc caagatcctg atcgccaacc gtggtgaaat
     2821 cgccctgcgg atcatccgcg cctgcaaaga attgggtatt cagaccgccg tcgtttattc
     2881 gaccgccgac aaggacgccg cgtatctgca attggccgac caggcgatct gcatcggcaa
     2941 cgcgccgccg gccgaaagtt acctgaacat cccgcgcatc atcagcgccg ccgagattgc
     3001 cgacgtgcag gcaattcatc cgggctacgg attcctagcg gagaattcgc acttcgccga
     3061 agtctgccgc agttgcaaga tcgagttcat cggcccaccc gtgaaggcga tggccgcggt
     3121 gggtgacaag gtcgcgtgca agaagctggc gaaaaaagcg aaggtgccga ccgtgcccgg
     3181 ttccgacggc gcggtcgatg acgagaagat cgcgctgcgc ctggccagcg agatcggtta
     3241 cccggtcatc atcaaggccg ccgccggtgg tgggggacgc ggcatgcgcg tcgcccacaa
     3301 cgatgtgtcg ctgcgcgcgg gtctgaagca agcgcaggca gaagcggaaa acgcattcaa
     3361 agactcgacg gtctacattg aaaagtacgt cgagttcggc cggcacgtcg aagtgcagat
     3421 tctcgccgac agtcacggca acgccgtgca cctgtgggag cgcgactgct cgatgcagcg
     3481 gcgtcaccag aagctggtgg aagagtcgcc gtcgcccgtg cttcgccagg aagtgcgcga
     3541 gcgcctctgc gacagcgctg ttcgcctcgt caaggccgcc ggctacacca acgccggcac
     3601 ggtcgaattc ctggtcgata aggaccagaa cttttacatc ctggaagtga acgcccgcat
     3661 ccaggtcgag cacccggtga ccgagcaggt gaccggcatc gacttgatca aggaacagat
     3721 ccgcatcgcc gccggcgagc cgctgtcgtt caagcagacg gacgtccggc aaatcggcca
     3781 cgcgatcgag tgccgcatca atgccgaaga ccccgcgaga aatttcgcgc catcccccgg
     3841 tttgatcatc gagctacgcg tccccggtgg ccctggtgtc cgcctggaca gccacgccta
     3901 cgccggctac cgaatcccgc ccaactatga cagcatgatc ggcaagctga tcgtgcaccg
     3961 aaacacccgg gccgaggcga tcgcgacgat gaagcgcgcc ctgggcgagt tccacatttc
     4021 gccgatcaag acgacgatcc cgctgcatct gcagatcatg gacaacgagt acttccgcag
     4081 cggtgacgta gacaccggct tcgtcgagcg ggtgctgctg cagaagtaga agtgggcaaa
     4141 gcttttcgcg ccgacaatgt gcgatcaatt cgaatagtgg ctatgctcgg attttatggc
     4201 ggttttaaat aaggtcgaca tcactgctgc gcttgcacaa ttgggtgaac tcgctttcgc
     4261 tgaaggactg actatcgagt cgctcattgt cggcggcgca gcgatgggct ggagttcgaa
     4321 gcgcggctat ccacgcgata cgcgttcgag gatccatggg aaagtcggcc atgacgttga
     4381 tcgaagatct ggccgccgct gccttggccg gagatggact aaccgcgcgc agcctgttgc
     4441 aggactttca gcgagcacgg ccacgtctag ccgatgttcc gccgccttcg tcacgtgatc
     4501 cgcgtgttcg ctcgctcgct gccgcgctgg ttgaaatgat ggcggagcgc aatggtgaac
     4561 cgcctccgcg gtggacggcg gacattggtc cagtggccga gccaatccac cttctcaaat
     4621 ccgctgatcg gatgtctcgc ctgcgtcatc tgtgcgaaga acagtcgccg gaaccgcttc
     4681 gccggcgcag gttctttgcg ccaccgaatt atctgcaatc ggtatgatgg caatgcgcac
     4741 tcacgctgtg gattacgctt cgctggttga ataatgcgac acgtcattga gcagcgtcac
     4801 gcgcccgctc atcgattcgc agaactcgaa cacgttcaag cacgccgtgt cttgcgagat
     4861 ccgtgcccga tacatctgcg gatcgatccc catgatgctg cacagcagca gccggtttgt
     4921 tgccttgtgc gagaccaccg cgaccgtctg gtccggatga tccaatacaa tccgccgcag
     4981 ctccggcagg ctccgcgcga gtaccgtcaa ccccgtctcg ccacccggcg gggcgacgag
     5041 aaacgggttc gcctcccacg cctgatactc cgccgcgtac tgctgctcga ccgccttgtg
     5101 caccatgcct tcccactggc cgtgatcgat ctcgcgcaac cccgcccgca cgatcggcgt
     5161 cagcccatgc ggccgcgcca ccgcggcggc ggtgtccatc gcacgatgca tgtcgctgca
     5221 atacaccgcc gcgatctttg tagctgccaa tcgaaggccg agccgctggg cctggcttcg
     5281 cccatcgtcc gacagctgaa catccgtcga accggcgaag cggtcttcca gcgtcaacac
     5341 cgtcgccccg tggcggatga gcaggacctt tgtcatatcg cgcatcatta ccgcccgatg
     5401 cgatgcgctc aacgcgcatc gacaatcccc cacgccgggt tcggaggtac gccgacgacc
     5461 cgggtggtct tgaatgcaga atgatgaatg cagaatggcg aatgaatact ggccgcttcc
     5521 gtctgcattc tgcattcatc attctgcatt cctgcatttg caatttgcgt ccgcgccgcc
     5581 tcgccgatac cttccccgca tcccgctggt cgccgcgcgg acgggttagc cgcgcgtggg
     5641 ttcgaccagt catgtcgggc cggcgcgctg caactttgag cgcgcgcggg ccctcgtgcg
     5701 gagaaacgat gcgtctgtcg ttacgcgcca cgttgttggc tgcgctgttc ttattctgtc
     5761 tgtcgccgat catcgcgcgc gctgacctgc cgccgatgcc cgagacggct catccgatcg
     5821 actccggcgc caccgcgtgg atgctcacct ccaccgcgct ggtcctgctg atggtcccgg
     5881 gcctggcgat gttctacggc ggacttgtcc gcaccaagaa cgtcctcggc acgatgatgc
     5941 acagcttcag cgccatggcg gtcgtcggcg tcctctgggt cgtcatcggt tacgcg
//
LOCUS       DATIMH010000260         1588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_924111, whole genome shotgun sequence.
ACCESSION   DATIMH010000260 DATIMH010000000
VERSION     DATIMH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924111"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VLI90_04400"
     CDS             complement(<1..253)
                     /locus_tag="VLI90_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13474.1"
                     /translation="MAGHSDIAPAIPGAEPKLASDALFAAPVASRRGIVAIGLVVFIA
                     FSDIALFVATANPLIAAAPAIAAALAFAIWKTPLRHLIYA"
     gene            complement(441..>1588)
                     /locus_tag="VLI90_04405"
     CDS             complement(441..>1588)
                     /locus_tag="VLI90_04405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV13475.1"
                     /translation="DSRPVEQRVNRFVNALRGRRGGGLTTVTAFSAAAYYYFERLVWR
                     EFGERIRGGEFDVVHRLTPLSPTTPSLLAKKVARAGVPFVLGPLNGGLPWPPGFNYAR
                     VQEHEWLSFVRGGYKLLPGYRGTRNHASAIICGSAATRAQISPRYRDKTVYIPENAVD
                     PARFDERASPVTTLPLRVAFVGRFTLYKGCDMLIEAAAPLVRAGQVQLDLIGDGAQMP
                     ALRTLAARLGVPDASFAGWVPHGTLKQRLSRSHVFAFPSIREFGGAVVLEAMALGLVP
                     IVMQYGGPGELVSPATGFRLPMGRRDEIIAALREQLEQLAAAPERIATIAARARDRVL
                     RHFTWEAKAAQVHEVYRWVLNERDKPDFGMPLPDEAAEVMTTAAVGG"
BASE COUNT          288 a          544 c          531 g          225 t
ORIGIN      
        1 gggcatagat caggtggcga agcggcgtct tccagatcgc gaacgcgagc gcagcggcga
       61 tcgccggcgc cgcggcgatc agcggattcg ccgtcgcgac gaacagcgcg atgtcactga
      121 acgcgatgaa caccaccagc ccgatcgcca cgatcccgcg tcgcgaagct accggtgcag
      181 cgaacaatgc atcgctcgcc agcttcggtt cggcgccggg aatcgccggc gcgatgtcac
      241 tgtgaccagc cacgcgatac ggcatctagg acaggcgcgg aaaaacgtag acgaccagga
      301 cgacatagac cgcgacgaca gcggccatcg tcgcgatggt ccggcgcagt ccgcggaccg
      361 ccgaccggtc gcgcgcgaat cgcatcggca cggcgaacgt cgagatcagc agcgacagca
      421 gcagcagctt gcgcaggtcc tcaccctccg acagcagcgg tggtcatcac ttcagccgct
      481 tcatcgggga gcggcatgcc gaagtcgggt ttgtcgcgtt cgttgaggac ccagcggtac
      541 acctcgtgta cctgcgcggc cttcgcttcc cacgtgaaat ggcgaagcac ccggtcgcga
      601 gctcgtgcgg cgatcgtcgc gatccgctcc ggcgcggcgg ccagctgctc gagctgctcg
      661 cgcagtgccg cgatgatctc gtcgcgacga cccatcggca ggcggaaacc ggtcgctgga
      721 ctcacgagct caccgggacc gccgtactgc atcacgatcg ggacgagacc cagcgccatc
      781 gcctcgagca ccaccgcacc cccgaactcg cggatgctgg ggaacgcgaa cacgtgcgac
      841 cgggacaagc gctgcttgag ggtgccgtgc ggtacccatc cggcgaagct cgcatcgggt
      901 acccccaacc gcgcggcgag cgtccgcagc gcgggcatct gcgcgccgtc cccgatgaga
      961 tcgagctgca cctggccggc ccgcacgagc ggagccgccg cttcgatcag catgtcacac
     1021 cctttgtaga gcgtgaaccg gccgacgaag gcgactcgca ggggcagggt cgtcaccggt
     1081 gatgcacgtt cgtcgaaacg cgctggatcc accgcgttct cggggatgta gacggtcttg
     1141 tcgcggtaac gcggcgagat ttgcgctcgc gttgcagccg aaccgcagat gatcgccgat
     1201 gcatgattgc gcgtcccgcg gtaacccggc agcagcttgt agccgccgcg cacgaaactc
     1261 agccactcgt gctcctggac gcgcgcatag ttgaagccgg gtggccaggg caagccgccg
     1321 ttcagtgggc cgagcacgaa cggcacgccg gcgcgtgcga cttttttcgc gagcaggctc
     1381 ggcgtcgtcg gcgacagcgg cgtcaggcga tggacgacgt cgaattcgcc accgcggatt
     1441 cgctcgccga actcgcgcca gacgagccgt tcgaagtagt agtaggccgc agcgctgaag
     1501 gccgtgacgg tggtcagccc gccaccacgc cgcccgcgca gcgcgttgac gaagcgattc
     1561 acccgctgct cgaccgggcg cgaatcga
//
LOCUS       DATIMH010000261         6041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4968072, whole genome shotgun sequence.
ACCESSION   DATIMH010000261 DATIMH010000000
VERSION     DATIMH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4968072"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /gene="nusA"
                     /locus_tag="VLI90_04410"
     CDS             complement(<1..1047)
                     /gene="nusA"
                     /locus_tag="VLI90_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HSV13476.1"
                     /translation="MNPELIRIVDGIARDKSIEREQIYTDIEQAIASALRKQFNTEDT
                     SEFTVTLDRTTGDISAARQGNPMPLSILGRIAAQTAKQVMIQLIRQDERGSIYEEYKD
                     RVGTIVTGTIARFEGGSMIVTLGRNEGIMPRSEQIPGETHQVGERVQALILEVRDTPS
                     AVKIILSRSHPELIRRLFEREVPEVGERTIEIKALAREPGHRTKIAVSSIDSKVDAVG
                     ACVGVRGSRIKNIVDELGGEKIDIVRWNESSQILISNALKPAEVAEVSLCFELGRATV
                     IVNDDQLSLAIGKRGQNVRLAARLTGWDIDILTPPEFQNGVQRLDLTLKSIEGITQEM
                     VDKAIALGLIDVRDI"
     gene            complement(1258..2508)
                     /gene="ugpC"
                     /locus_tag="VLI90_04415"
     CDS             complement(1258..2508)
                     /gene="ugpC"
                     /locus_tag="VLI90_04415"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HSV13477.1"
                     /translation="MARVELEKVSKIYAGGVKAVDAIDLVIKDQEFIVLVGPSGCGKS
                     TTLRMVAGLEEISEGVIKIGDRIVNDVPPKDRDIAMVFQNYALYPHMSVYKNMAFGLK
                     LRGMPKAQINERVMQAAKILDIEHLLERKPKALSGGQRQRVAVGRAIVREPAAFLFDE
                     PLSNLDAKLRVTTRAELKRLHQRLKTTTIYVTHDQEEAMTLGDRIVVMKDGLIQQADT
                     PLNTYNYPANRFVAGFIGMPPMDFFDGAIKLVDGQMVFEEGKLENAREATRGAGGKAS
                     DEPVVLVGELTLPGSGFTLPIPDRMKDALAGKVGSHVVLGIRPEHLHVRQTEGAMPMK
                     VTMNVIEPLGNDMDVYMKTALRDHVVGRMEAEQGLQMGTLTTVYADVRKSHFFEPGVT
                     GMNLSLTAAGAKSAGLKETTHAIA"
     gene            complement(2711..3136)
                     /locus_tag="VLI90_04420"
     CDS             complement(2711..3136)
                     /locus_tag="VLI90_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HSV13478.1"
                     /translation="MSDEWVEIRGDALPVAEAIAFTSTPAAGGIDVFLGTTRGETNSD
                     GRELIALDYEAYAAMADQQFRSFAKQARQRWPIVRLVILHRTGRVGLGEPSVLIAVST
                     PHRAESFEACHWLIDTLKKEATIWKKQVWQDGAGEWVHP"
     gene            complement(3133..3384)
                     /gene="moaD"
                     /locus_tag="VLI90_04425"
     CDS             complement(3133..3384)
                     /gene="moaD"
                     /locus_tag="VLI90_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HSV13479.1"
                     /translation="MHITVKLFAVLRDRAGTNQIDLELPERATAMDASRRIGERYPAI
                     ADFLPRVAHAVNQAYVPASTVLNDGDELALIPPVSGGCA"
     gene            complement(3494..4372)
                     /gene="lpxI"
                     /locus_tag="VLI90_04430"
     CDS             complement(3494..4372)
                     /gene="lpxI"
                     /locus_tag="VLI90_04430"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HSV13480.1"
                     /translation="MTPLGLIAGEGVFPILVARGARAAGRKVVCVGLAGNAWPQLREE
                     CDEFHWAGISRLGGWVRRLRASGCTEAIMVGRVSKAKMYTPFVLLRYIPDLRTARVYL
                     QQLRHDKRPNAVLRAIIDVLGSSGIHLIDSTSYCKDQLATPGVMTRRQPSDDQWRDIR
                     FGWQICKQISTMDIGQSIAVLDCDVIAVEALEGTNAMIERAGGLCKCGGWTLIKVANK
                     NQDMRADVPSIGTVTIDKLAAAGASCLVLEAGKTIMLEKQKVLELADRHKIVIVGYEG
                     EPAAKLEQPQAAQAMS"
     gene            4576..>6041
                     /locus_tag="VLI90_04435"
     CDS             4576..>6041
                     /locus_tag="VLI90_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13481.1"
                     /translation="MPDVRPNHLRRREFEPLEDRLLLSVSQDANGWSVVTPSADSQLI
                     YVSSSLGNNSNNGLTPSSPVKTFAKAQSLMRNGFPDQLLLRRGDTFVDYFTNWSKSGR
                     SANEPILIGNYGTGARPVVDTSGQFGGFATLDTSTVNYLDIIGVEFINQVHNPNSPTF
                     SFSNQSGVTGLEFGCAGGNLLVENCSFRYYRNNLDIEGLHGPLGNITIRRSLIADSWS
                     NTSAHSQGMYGNEIDNVAITECVFDHNGWNEQITGAGAQGYNHNIYLSSLTTNADIEN
                     NILSNASFAGVMDRCGGIVKNNVFINDAIAVSFGNADGANSTAGGVTGEITGNIVLGD
                     KGGVSYGEGIEVGNTKPGANVPVQNNIIVGDTIHTAAAITLTMATNTLNPSQAVGLND
                     VTLQNNIEYGWYRGMMIDGRFSPGGSGLYALNNLNIRNNDFIGSTNRLIRQDGRFVRE
                     QEHWAGNRYYTSALSQSSWFLLISNVLSFKQWKSAFDPA"
BASE COUNT         1144 a         1972 c         1710 g         1215 t
ORIGIN      
        1 gatgtcgcgc acgtcgatca ggcccagggc aatggccttg tctaccatct cctgcgtgat
       61 gccttcgatg ctcttgagcg tcaggtccaa ccgctgcaca ccgttctgga attccggcgg
      121 cgtgaggatg tcgatgtccc aacccgtcag ccgcgcggcc agccgcacgt tctgtccgcg
      181 cttgccgatc gccagcgaca attgatcgtc attaacgatg accgtcgcgc gaccgagttc
      241 gaagcagagc gagacttccg cgacttccgc aggcttgagc gcgttgctga tcaggatctg
      301 cgaggactca ttccagcgga cgatgtcgat cttctcgccg cccaattcat cgacgatgtt
      361 cttgatgcgg ctgccgcgca cgcccacgca cgcgccgacg gcgtcgacct tcgaatcgat
      421 ggacgacacg gcgatcttcg tgcgatgacc cggctcgcgc gccagcgctt tgatttcgat
      481 cgtgcgctcg ccgacttccg gcacctcgcg ctcgaacagc cggcggatca gttccgggtg
      541 gctccggctg aggatgatct tcacggcgct gggcgtgtcg cgcacttcca agatcagcgc
      601 ctgcacgcgc tcgccgacct ggtgcgtttc acccgggatc tgctcgctgc gcggcatgat
      661 gccttcgttg cggccgagcg tgacgatcat gctgccgcct tcgaagcggg cgatcgtgcc
      721 ggtcacgatt gtgccgacgc ggtctttgta ttcctcgtaa atgctgccgc gttcgtcctg
      781 gcggatcagc tggatcatca cctgcttggc ggtctgggcg gcgatgcgac cgaggatgct
      841 cagcggcatc gggttgccct gccgggcagc gctgatgtcg ccggtggtgc ggtcgagggt
      901 cactgtaaac tcgctggtgt cctcggtgtt gaactgcttg cgcagcgcag aggcgatcgc
      961 ttgttcgatg tcggtgtaga tctgttcgcg ctcgatgctc ttgtcgcggg cgatgccatc
     1021 tacgatgcgg atcagttcgg gattcatgct tcctcgctat tcagttgtcg tgatcgcgcg
     1081 cgaccgatgg tcgcggcttt acaacagaca cataaacaaa ttgggccgcc tttttgtagg
     1141 cagcccactc tcttcgacgg aacctgttca aacgccgaca ccctcacccg gagggaaaat
     1201 ttttcctcca ggtaagggcc gcagttgccc cgaagatgga cccacgctta tgcattgtta
     1261 tgcgattgca tgggtcgtct ctttcaggcc ggcgcttttg gcgccggcag ctgtgaggct
     1321 caggttcatc ccggtcactc cgggttcgaa aaagtgggac ttgcggacgt cggcatacac
     1381 cgtcgtcaac gttcccattt gaaggccttg ctcggcctcc atccgtccaa ccacatgatc
     1441 gcgcagggca gtcttcatgt atacatccat gtcattgccc agcggttcga tcacgttcat
     1501 cgtcactttc attggcatcg caccttcggt ctgccgaacg tgcagatgct ccggccgaat
     1561 gcccagaacc acatgactgc cgaccttgcc ggccagggca tctttcatgc gatcagggat
     1621 gggtagggta aacccgctac ccggcaaggt caactcgccg accaagacaa ccggctcatc
     1681 cgacgccttg ccaccggccc cgcgagtcgc ctcgcgggcg ttctccagct tgccttcctc
     1741 gaacaccatt tgcccgtcga ccagcttgat cgccccgtcg aagaagtcca tcggaggcat
     1801 gccgatgaag cccgcgacga atcggttggc cgggtaatta taggtgttca acggcgtgtc
     1861 ggcttgctga atcaggccat ctttcatcac gacgatgcga tcgcccagcg tcatggcttc
     1921 ttcctggtcg tgcgtgacgt aaatggtcgt ggtcttcagc cgctggtgca gccgcttgag
     1981 ctcggcccgc gtcgtcacgc gcagcttggc atcgaggttc gacaatggtt catcgaacaa
     2041 gaatgcggcg ggctcgcgca caatcgcgcg accgactgcg acgcgctggc gttgaccgcc
     2101 cgaaagcgct ttgggctttc gttccagcag atgttcgatg tccaggatct tcgccgcctg
     2161 catcacgcgt tcgttgatct gcgccttggg catcccgcgg agcttcaacc cgaaggccat
     2221 gttcttgtac acgctcatgt gcgggtagag cgcgtagttc tggaagacca tcgcgatgtc
     2281 gcgatccttg ggcggcacat cgttgacgat ccggtcgccg attttgatga cgccttcgga
     2341 aatctcttca agccccgcga ccatgcgcag cgtggtgctc ttgccgcaac cggacgggcc
     2401 gacgagcacg atgaattcct gatccttgat gaccaggtca atcgcgtcga ccgccttcac
     2461 accgcccgcg tagatcttgc tgaccttctc gagttcaact ctggccatgc gtgtcaccgc
     2521 tccgataggg gtacagatcc gccgaaagga agggggatta tacagaatgc ttgatgagga
     2581 tcaaaacccg aggggcacaa agcggtgcaa tcgatacgcc gcttgcggct tcgcaatcca
     2641 ccacccatgc aatagtaggt tttgcgatcc cgccgagtcg aattgcgaag ccgcaagcgg
     2701 cggaggtgat ttacggatgc acccactcgc cggcgccgtc ttgccatact tgctttttcc
     2761 agatggttgc ttccttcttc agcgtgtcga tgagccagtg acatgcttca aacgactccg
     2821 cccgatgcgg cgtgctcacc gcgatcagga ccgagggttc acccaaccca acccgccccg
     2881 tgcgatgtag gatgaccagc cgaacgatcg gccagcgctg ccgggcctgt tttgcgaatg
     2941 accggaactg ctgatccgcc atcgccgcgt acgcttcata atccagcgcg atcaactctc
     3001 gcccgtctga gttggtctcg ccgcgagtgg tacccagaaa cacatcgatc ccccccgccg
     3061 cgggtgtcga ggtgaacgct atcgcttcag caactggcag cgcatcgccg cgaatttcca
     3121 cccattcatc gctcacgcgc agcctccact caccggcggg atgagcgcca gttcatcgcc
     3181 gtcgttcaga accgtcgaag ccgggacgta cgcttggttg acggcatggg ccacccgcgg
     3241 caggaagtca gctatcgcgg gataacgctc gccgatgcgg cgcgacgcgt ccatcgccgt
     3301 ggcgcgctcc ggcaactcga gatcgatctg attcgtcccc gcgcggtcgc gcagaacagc
     3361 gaacagcttt acggtgatgt gcatggttgt catcacgccg agttcggagc gtactcgcga
     3421 cgaagcgaga ttcttccaac ttcgcaaaaa gcatccgggc cgtcggagta cctccgaacc
     3481 cggcgtggtt tgatcatgac atcgcctgcg ccgcctgcgg ctgctccagc ttcgcggccg
     3541 gttcgccttc gtagcccacg atgacgatct tatgccggtc ggccagctcc agcacctttt
     3601 gcttttccag catgatcgtc ttgcccgcct ccagcaccag gcacgatgcc cccgcggccg
     3661 cgagcttgtc gatcgtcacg gtgccgatgc tcggcacatc cgcgcgcata tcctggtttt
     3721 tgttggcgac cttaatcaac gtccatccgc cgcacttaca caggccgccg gcacgctcga
     3781 tcatggcatt ggtgccttcg agcgcttcaa ccgcaatcac atcgcaatcc agcaccgcga
     3841 tggattggcc gatgtccatc gtgctgattt gcttgcagat ctgccaaccg aagcggatgt
     3901 cgcgccactg gtcatcgctg ggctgccgcc gggtcatcac gcccggcgtg gctaactggt
     3961 ctttgcaata gctggtcgag tcgatcaagt gaatccctga agaaccgagc acatcaatga
     4021 tcgcacgcag caccgcgttg ggccgcttgt cgtgacgcaa ttgctgcagg tatacgcgcg
     4081 cggttcgcag gtctggaata taccgtagaa ggacgaacgg cgtgtacatc ttcgccttgg
     4141 acacccgacc gaccatgatc gcttcggtac accccgatgc gcgcaaccgc cgaacccacc
     4201 cacccaggcg ggaaatgccg gcccagtgga actcgtcgca ttcctcgcgc agctgcggcc
     4261 aggcgttgcc ggccagaccg acgcagacca cctttcgccc cgccgcccgc gcgccgcgcg
     4321 caacgaggat cggaaagacg ccctccccgg cgatcaaacc cagcggtgtc atctgactga
     4381 ggataataat cgctcgccct caatgtgtga aatcgcgcgg tttcgaatgc aaatttcccg
     4441 ctaattgatt gaccaaacac cgtccgattc ttagattagc tggactgtcg ttctgcattc
     4501 tgtacgggcg ttcccccctc cgctaattga cgcgcgccac ggcacaacca gttgtcatcg
     4561 caccgggacc aaccattgcc tgacgtacgc ccgaaccatc tccgccgtcg cgagttcgag
     4621 ccgctggaag accggctgct gctgtccgtc tcacaagacg ccaacggctg gagcgtcgtt
     4681 acacccagcg ccgattcgca actgatctac gtcagctcat cgctgggcaa caatagcaac
     4741 aacggcctga ctccatcatc gccggttaaa acattcgcca aagcgcagtc gctgatgcgc
     4801 aacggcttcc ccgaccaatt gctgcttcgc cgcggggaca cgttcgttga ctacttcacc
     4861 aactggagca agtccggccg cagcgcgaat gagccaatct tgatcggcaa ctacggcacc
     4921 ggcgctcggc ccgtcgttga caccagcggg cagttcggcg gcttcgccac gctcgatact
     4981 tcgaccgtca actacctcga catcatcggc gtcgaattta tcaaccaggt tcacaacccc
     5041 aactcgccca cgttcagctt ctccaaccag agcggtgtga ccgggctgga gttcggctgc
     5101 gcgggcggga acctgctggt cgagaactgc tccttccgct actaccgaaa taatctcgac
     5161 atcgaaggtc tgcacggccc gctcggcaac atcaccatcc gccggtcgct catcgccgac
     5221 tcctggtcca acaccagcgc ccattcgcag ggcatgtacg gcaacgagat cgacaacgtc
     5281 gccatcacag aatgcgtgtt cgaccacaac ggctggaacg agcagatcac cggcgccggc
     5341 gcacaggggt acaaccacaa catctacctg tcgtcgctga cgaccaacgc cgacatcgaa
     5401 aacaatattc tctccaacgc ctccttcgcc ggcgtcatgg atcgctgcgg tgggatcgtg
     5461 aagaacaacg tcttcatcaa cgatgcgatt gcggtctcct tcggcaacgc cgacggcgcc
     5521 aacagcaccg cggggggtgt gaccggcgag atcaccggca acatcgtgct cggtgacaaa
     5581 ggcggcgtgt cgtacgggga agggatcgaa gtcggcaaca ccaagcccgg cgccaacgtg
     5641 ccggtgcaga acaacatcat cgtcggcgac acgatccaca ccgccgccgc catcacgctg
     5701 acgatggcga ccaacaccct gaatcccagc caggccgtcg ggcttaacga tgtcacgctg
     5761 cagaacaaca tcgagtacgg ctggtaccgc gggatgatga tcgatggccg gttcagcccc
     5821 ggcggcagcg ggctgtatgc gctaaataac ctgaacatcc ggaataacga cttcatcgga
     5881 tcgaccaacc gcctgatccg gcaggatggg cggttcgtcc gcgagcagga acactgggcc
     5941 ggcaatcgct attacacctc ggcgctgagc caatcatcgt ggttcctgct gataagcaac
     6001 gtgctgtcgt tcaagcagtg gaagtcggcg tttgatcccg c
//
LOCUS       DATIMH010000262         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4968120, whole genome shotgun sequence.
ACCESSION   DATIMH010000262 DATIMH010000000
VERSION     DATIMH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4968120"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..1477)
                     /locus_tag="VLI90_04440"
     CDS             complement(296..1477)
                     /locus_tag="VLI90_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13482.1"
                     /translation="MHTILAAAVSFLTFCGMLRADPPTTAPLEAQRAEERKGIEKQYA
                     DAPVLLPAKLSDAVSFQLNKNDLMVYTRLAPTNGPARLNLPELPGICVMDTRGPQPRL
                     HQPYTPVSMLFIHFDFSKPGTSISSTTVQVTPMNVQVSQDMEWPGGGVRQVSLVQNLP
                     GINDSEAPVRMSVSLIGNPSEAPATQAADANNDGSAMVKIEHTAPDFVTLRRQYPGDA
                     AKYLEPIFRELHADATLFGPDPKLAWQVFLTEAPETPQVTQQVKRIVAQLDSDDVHER
                     DAAAAHLAELGDPAAVSLMHMDRSAWSPEQSSRVDAFLSSYRLVSDEDAHRLRDDVNF
                     LLDCLYDTDDFTVHCALKRLGEITGHPVAFDASLRDEARRNAVNTLREQLAPTTPKPV
                     K"
     gene            complement(1532..2074)
                     /locus_tag="VLI90_04445"
     CDS             complement(1532..2074)
                     /locus_tag="VLI90_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HSV13483.1"
                     /translation="MSRKAAQDWPPGRELELYIKPAMPSPVLIEKAGDTIASFTYKEV
                     PLAYWLLKTEPDCYAWDDLARDKKTTWDGITNALALKHIRTMKKGDLALIYHTGGERA
                     AVGIAEITTNPHADPKEDDEKLAVVDLKPKGKLPRPVTLDEFKADEAFAGWDLLRIGR
                     LSVVPVPDAMWKRIEALAKE"
     gene            complement(2028..2417)
                     /locus_tag="VLI90_04450"
     CDS             complement(2028..2417)
                     /locus_tag="VLI90_04450"
                     /inference="COORDINATES: protein motif:HMM:NF017902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HSV13484.1"
                     /translation="MPTDTQQIMDAAEKLSQMLADHPAVARYKAAQKAVTEDAEASRL
                     LGEFDQQIERLARQEQTGMPITDAQRQQLESLQTRIVSHLKIKNLNLAQVEFVDLLRK
                     VNQTIQRPLTETGVAQGGARLAAGPRA"
     gene            2530..3567
                     /locus_tag="VLI90_04455"
     CDS             2530..3567
                     /locus_tag="VLI90_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HSV13485.1"
                     /translation="MDRYHRQTLLPFIGEAGRDRLSASRVLLVGCGALGSVIADQLVR
                     AGLGELIIADRDVVEMTNLQRQVLFDEQDARDGVPKAVAAPRRLAAVNSTVRIEPRVV
                     DVHSGNIEALAGLEAGGTRVDLILDGTDNAQTRYLVNDVAVKHGVPWVYGACVGADGR
                     VMSVRPGVGPCLRCVFPVPPSAGELPTCDTAGVLASATAVVASMQVVASLKLLLGASL
                     ADDRLLAMDLWNGRFRSIAADRQEHCVCCTQRQFEFLDRPIEQSAIQLCGQNAVQIRA
                     ERGERIELKALAERLTPLGHVTVTDFLVRCRPTQERGVQLTVFADGRTVVQGTSDPSR
                     ARILVSRYVGL"
BASE COUNT          609 a         1062 c         1259 g          764 t
ORIGIN      
        1 ggtgatgccg atcgtctggg cgttgaccca gtagatgaca aagagcgtga cggcgacggg
       61 gatgacggcg aaaatcccgg cgaggaacgc gttgcgcagg tgagcttgga gccgatgcat
      121 gtggcgcatt ctgacgggga ggtgcgggtt agcaagtgga cgaggcgctc gcgcgcacac
      181 cgttgacagc cgcgggcgat agccagcggg gcgcggcagt tcgatgcggg tttgcgtatt
      241 cgagagtccc cgctggctgt cgcctgcggt tgtgaaagac aaggcggtcg ttcaactact
      301 tcacgggctt cggtgtggtc ggcgccagct gctcgcgcag cgtgttcacc gcgttgcgcc
      361 gcgcttcatc gcgaagcgat gcatcgaacg ccacgggatg tccggttatc tcacccagac
      421 gcttcagcgc gcaatgcacg gtgaagtcgt ccgtgtcgta caggcagtcg agcaggaagt
      481 tcacgtcatc gcgcaggcgg tgagcgtctt cgtcggagac caggcgatac gacgagagga
      541 acgcgtccac gcggctgctt tgttcgggag accacgccga gcggtccatg tgcatcaggc
      601 tgaccgcggc ggggtcgccg agttcggcca ggtgtgcggc ggcggcgtcg cgctcgtgga
      661 cgtcatccga atccagctgc gcgacgatgc gcttcacctg ctgcgtcacc tgcggagttt
      721 cgggcgcttc ggtaaggaag acttgccatg caagcttggg atcgggaccg aacagtgtcg
      781 cgtcagcgtg cagttcgcgg aagatcggct cgagatactt ggcggcgtcg ccggggtatt
      841 ggcggcgcag cgtgacgaaa tcaggcgcgg tgtgttcgat cttcaccatc gccgagccgt
      901 cattattcgc gtcggcggct tgcgtggccg gcgcttccga tgggttcccg atgagtgaga
      961 cggacatgcg gactggcgct tcgctgtcgt tgatgccggg caggttctgc acgagcgaca
     1021 cctgccgcac accgccgcca ggccattcca tgtcttgcga gacttgcacg ttcatcggtg
     1081 tgacctgcac ggttgtcgat gagatgctcg tgccgggttt ggaaaagtcg aagtggatga
     1141 acagcatgct gaccggcgtg tatggctggt gcagccgcgg ctgcgggccg cgcgtgtcca
     1201 tcacgcaaat gccgggcagt tctggcaagt tcaagcgcgc tggcccgttg gtcggcgcca
     1261 gccgggtgta gaccatcagg tcgttcttgt tcagttggaa cgagacggcg tcgctgagct
     1321 tcgccggcaa cagcaccggg gcatcggcgt attgcttctc aatgcccttt cgctcctcgg
     1381 cccgctgggc ttccagcggc gcggtggtgg gtggatcggc gcggagcatt ccgcagaacg
     1441 tcaaaaacga aacggcggcg gcgagaatcg tgtgcaagcg aacgcgcata cgctttggaa
     1501 atagtagcac cgccgctcgc cgaagacgcg gtcattcctt ggcgagcgct tcgatccgtt
     1561 tccacatcgc atcgggcacg gggacgacgc tgagccggcc gatgcgcagc aggtcccagc
     1621 cggcaaaagc ctcgtcggcc ttgaactcat ccagcgtcac cgggcggggg agctttcctt
     1681 tcggtttcag atcgaccacc gcgagcttct cgtcatcttc cttcggatcc gcgtgtggat
     1741 tggtggtgat ctcggcgatg cccacggcgg cgcgttcgcc gccggtgtga tagatgaggg
     1801 cgaggtcgcc ctttttcatg gtgcggatgt gcttgagcgc gagggcgttg gtgatgccgt
     1861 cccaggtggt tttcttgtcg cgcgcgagat cgtcccaggc gtagcagtcg ggttcggtct
     1921 tgaggagcca gtaggccaac gggacttcct tatatgtaaa gctggcgatg gtatcgccgg
     1981 ctttttcaat gaggaccggc gatggcatcg ccggctttat atacagttca agctctcggc
     2041 ccggcggcca gtcttgcgcc gccttgcgcg acaccggttt ccgtcagcgg ccgctggatc
     2101 gtctggttca ccttgcgcag cagatcgacg aactcgacct gcgccaggtt caggttcttg
     2161 atcttcaggt gcgacacgat ccgcgtctgc agcgattcca gctgctggcg ctgggcatcg
     2221 gtgatcggca tcccagtctg ctcctgtcgc gccaggcgtt cgatctgctg atcgaattcg
     2281 ccaagcaatc ggctggcctc ggcatcttcg gtgacggctt tctgggcggc cttgtagcgc
     2341 gcgacggccg ggtggtcggc cagcatctgc gaaagcttct cggcggcgtc catgatctgc
     2401 tgcgtgtcgg taggcatgcg aatccttgag tttgcgggcg tttgcgaagg gagtcactat
     2461 aatcgagccg cccggtcggg caaatgacag aaagcacgcg ctccggcgtg tgcccgcggc
     2521 tggcgattca tggaccgtta tcaccgtcaa actctgctgc cgttcatcgg cgaggccggt
     2581 cgagaccggc tgtcggcgtc gcgcgtgctg ctcgtgggtt gcggcgcgct tggatcggta
     2641 atcgccgatc aactcgtgcg cgcgggcctt ggcgagctga tcattgcaga ccgcgatgtc
     2701 gtcgagatga cgaaccttca gcgccaggtt ttgtttgacg agcaggacgc ccgcgatggc
     2761 gtgcccaagg ccgtcgcggc cccgcggcga ttggctgcgg ttaactcgac cgttcggatt
     2821 gaaccacgcg tcgtcgatgt tcattcgggg aacatcgaag ccctggcggg actggaagct
     2881 ggtggcacgc gcgtcgactt gatcctcgac ggcaccgaca acgcgcagac cagatatcta
     2941 gtcaacgatg tggctgtgaa gcacggcgtg ccgtgggtgt atggcgcgtg cgtcggcgcg
     3001 gatggccggg taatgagcgt tcgccccggt gtcggcccgt gtctgcgctg cgtattccca
     3061 gtaccgccat cagctgggga actgccgaca tgcgatacgg caggcgttct cgcttcggcg
     3121 acggctgtcg tcgcgtcgat gcaggtggtt gcttcgctga agttgctgct tggtgcgtcg
     3181 ctagcggatg atcgattgct cgcgatggat ctgtggaacg ggcggtttcg ctcgatcgct
     3241 gcggatcgac aagaacattg cgtctgctgc actcagcgtc agtttgagtt tctcgaccgg
     3301 cccattgagc aatccgcgat tcaactctgc ggccagaacg ccgtgcagat tcgcgcggag
     3361 cgaggcgagc gaatcgaact caaagccctt gcggagcggc tgacgccgct ggggcacgtg
     3421 acggtcaccg actttctggt acgctgccgc ccaacgcagg aacgcggcgt gcaattgacc
     3481 gtcttcgccg atggtcgaac ggtggttcaa ggaaccagcg atccgagccg agcgagaata
     3541 ctcgtcagcc gatatgttgg cctctgatcg gtagttatgg aagattgatt tattcactac
     3601 ttccccctag gattggtagg aaatgcctgt ggatgtgctg acgcgaacac cttcgatgca
     3661 ccagcggtcg ctgctggaac tgatcggtaa cacg
//
LOCUS       DATIMH010000263         5623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2946065, whole genome shotgun sequence.
ACCESSION   DATIMH010000263 DATIMH010000000
VERSION     DATIMH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2946065"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2615
                     /locus_tag="VLI90_04460"
     CDS             <1..2615
                     /locus_tag="VLI90_04460"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HSV13486.1"
                     /translation="ELGHFVAKLDPLGHDRPNHPLLELSNFGITSNLLDRPVSGGSFV
                     GWTGGTLRDLIEKLRATYCRTIGVEYTGISDKAQREWLTTRMEPILNRPEFSVTEARA
                     LMFQMIAAEEFENYLQTRFIGKKRFSLEGAESLIPLLNTIVEEGATVGAEKMIMAMAH
                     RGRLNALAHVLNKPYEVILSEFLETNLPPDQEGDGDVKYHLGYANTRPVANNKKVKVS
                     LLPNPSHLELIDPIQQGIIRCQQEIAGDKERTRVVPVTIHGDASFTGQGIISETLSLS
                     ELPGFRTGGTIHVIVNNQIGFTTPPRQGRFTPYPTDVAKMIQAPIFHVNGDDPEECVW
                     AAKLAIGFRQQFKCDVFIDMWCYRRHGHNETDEPEFTQPIMYRQIKSHKTTRQLYAEK
                     LIAEGKVSQQDLDAMKNIVIERLEQARNLAQEMKPRMKVPMFSGVWKGLGKAGSDWGA
                     KTGVTRDVLQKVTDAATRVPDGFTPHPKAARVLAGRAEMVKSGKGIDFGCAEMLAFGT
                     LLLEGTNIRFSGQDVERGTFSHRHDVLHDYKTGAVYTPLQHLTEKQARFNIRNTMLSE
                     LATLGFEWGYASADPRNLVCWEAQFGDFVNGAQSILDQIMASAESKWRYMNGMCLLLP
                     HGYEGQGPEHSNAYIERFLSLCAENNMQVVIPSTPASYFHVLRRQIHRKFRKPLVLFM
                     PKAMLRAGISTIDELIGDSHFQLVIDDPTNPAAADVTRVLLCSGKVYFSLATAREKEG
                     INDIAIVRVEQPYPFPAKEINGVLEKYRNARQIFWVQEETKNRGCWSFMESRVRELLP
                     QGAALGYIGRDEAASPATGSHKMHEVEEHEILTHALDLKQRAAAKPAAAQAAVAAVAT
                     SAPATAPSPIAASQ"
     gene            2748..4055
                     /gene="odhB"
                     /locus_tag="VLI90_04465"
     CDS             2748..4055
                     /gene="odhB"
                     /locus_tag="VLI90_04465"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF004309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HSV13487.1"
                     /translation="MPTNVDIPALGESVQEAVLLKWHKNNGDVVAENEPLCELETDKA
                     NVDIPAPAAGVLRRVKDEGATVHIGETIARIDESAGATGGNGSPAASKPVKAESAPAA
                     TPRITEAPKPPVEDLSPAVRRLVEENKLDPKQIAATGPGGRLTKEDVLKHIEGKSAAA
                     SAPTPAAPVAPSPAPAPTKAPSESIVAPPTSSKPIAGAMQFDAAGVHRAPMSKMRASI
                     ARRLVEAQHTAAILTTFNEVDLTEVISLRSKYKETFEKTHGIGLGFMSFFARAVVLGL
                     REFPRINAFIDGNDVVYHNYVHLGIAVSTDRGLAVPVLRNVQDMSFAKIESEIKRLAV
                     AARDGKLGIEELSGGTFTITNGGIFGSLLSTPILNPPQSGILGMHAIQKRAMVVGDKI
                     EPRSMMYVALSYDHRLVDGRESVSFLVRLKQLLEDPARLMLEI"
     gene            complement(4219..4974)
                     /locus_tag="VLI90_04470"
     CDS             complement(4219..4974)
                     /locus_tag="VLI90_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13488.1"
                     /translation="MKPVSVLSIACACAAGLTMVAAQSDAGVVQPGGQLSVTNQGPAH
                     DNSGDTAQGWTGYLLALVAPPGQMIEGISFGEPRSFGVPGNSSTLGIRGQMLQDWIPH
                     HGSHLSTPIGSSLSDANGSYNGTDSHLLTRSPITFQLSEDNDLVHPPGAPRNLITDTW
                     GTGHRLQGDTLFGPGDQLSVQPIAYLVLRNGSTATVAAEALPTGGGPEVGVDAEIVVP
                     RSVPLPAAPMLALSGLCAWGIPRAFRINSKARG"
     gene            complement(5173..>5623)
                     /locus_tag="VLI90_04475"
     CDS             complement(5173..>5623)
                     /locus_tag="VLI90_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV13489.1"
                     /translation="RYVIERVPVDAILSYCHYSLNDTSLLELIPTLKAKGVGVISASP
                     LSMALLSTRGAPDWHPAPPRVKQLCAQAAAHCRAKGASIEKLAVQYAVAQPDIATTVV
                     GTAEPKNMELNLAALDEPIDQQLLAEVLEILKPIHNVTWPSGRPENN"
BASE COUNT         1132 a         1850 c         1722 g          919 t
ORIGIN      
        1 gcgagctcgg ccacttcgtc gccaagctgg acccgctcgg tcacgaccgg ccgaaccatc
       61 cactgctcga gctgtcgaac ttcggcatca ccagcaacct gctcgaccgc ccggtcagcg
      121 gcggcagctt cgtcggttgg accggcggca ccctgcgcga cctgatcgaa aagctccgcg
      181 cgacctactg ccggacgatc ggcgtggaat acaccggcat ctccgacaag gcccagcgcg
      241 aatggctcac cacgcgcatg gagccgattc tcaatcggcc ggagttttcc gtcaccgaag
      301 cgcgggcgct gatgttccag atgatcgccg cggaagaatt cgagaattac ctgcaaacgc
      361 ggttcattgg gaagaagcgc ttctcgctcg aaggcgccga gtcgctcatt ccgctgctca
      421 ataccatcgt ggaagaaggc gcaaccgtcg gggccgaaaa gatgatcatg gcgatggccc
      481 accgcggccg gttgaacgcg ctggcccatg tgctgaacaa gccgtacgaa gtcatcctca
      541 gcgaattcct cgaaactaat cttccgccgg accaggaagg cgacggcgac gtcaagtacc
      601 acctcggtta cgccaacacc cgcccggtgg cgaacaacaa gaaggtcaag gtttcgctgc
      661 tgcccaaccc cagccacctg gagctgatcg acccgattca gcaaggcatc atccgctgcc
      721 agcaggaaat cgccggcgac aaagagcgca cccgcgtcgt ccccgtgacg atccacggcg
      781 acgcgtcgtt caccggtcag ggcatcatca gcgaaacgct gagcttgtcg gaactgcccg
      841 gcttccgcac gggcggcaca attcacgtca tcgtgaacaa ccagatcggc ttcaccaccc
      901 cgccgcggca ggggcgattc acgccttatc cgaccgacgt ggcgaagatg atccaggcgc
      961 cgatcttcca cgtgaacggc gacgatcccg aagaatgcgt gtgggcggcg aagctggcga
     1021 ttggcttccg ccagcagttc aagtgcgacg tgttcatcga catgtggtgc tatcgccggc
     1081 acggtcacaa cgagaccgac gaaccggagt tcacccagcc gatcatgtat cgccagatca
     1141 agtcgcataa gacgacccgc cagctgtacg ccgagaagct gatcgccgaa ggcaaggtca
     1201 gccagcagga cctggacgcg atgaagaaca tcgtgatcga acggctggaa caggcccgca
     1261 acctggcgca ggaaatgaag ccgcgcatga aggtgccgat gttcagcggt gtgtggaaag
     1321 gcctgggcaa agccggatcc gactggggcg caaagacggg cgtcacgcgc gatgtgctgc
     1381 agaaagtcac cgacgccgcg acgcgcgtgc ctgatggatt cacgccgcat cccaaagctg
     1441 ctcgagtatt ggccggtcgc gctgagatgg tcaagagcgg caaaggcatc gacttcggct
     1501 gcgccgagat gcttgccttc ggcacgctgc tgctggaagg cacgaacatc cgcttcagcg
     1561 gacaggacgt cgaacgcggc acgttcagcc atcgccacga cgtgctgcac gattacaaga
     1621 ccggtgcggt ctacacgccg ctgcagcacc tgacggaaaa gcaggcccgc ttcaacatcc
     1681 gcaacacgat gttgagcgag ttggcgacgc tcggattcga gtggggctat gcgtcggctg
     1741 acccgcgcaa cctcgtctgc tgggaagccc agttcggcga cttcgtcaac ggcgcgcaat
     1801 cgatcctcga ccagatcatg gccagcgccg aatcgaagtg gcgctacatg aacggcatgt
     1861 gcctgctgct gccgcacggc tacgagggcc agggaccgga acactcgaat gcgtacatcg
     1921 aacgattttt atcgctgtgt gccgagaaca acatgcaggt ggtgatcccc agcacgccgg
     1981 ccagctattt ccacgtgctt cgccggcaga tccaccgcaa gttccgcaaa ccgctcgtgc
     2041 tgttcatgcc caaggcgatg cttcgcgcgg gcatctcgac gatcgacgag ctgatcggcg
     2101 acagccactt ccagctggtc atcgacgatc ccaccaatcc tgccgcggcc gacgtgacgc
     2161 gcgtgctgct gtgcagcggc aaggtctact tcagcctggc aacggcgcgc gagaaggaag
     2221 ggatcaacga catcgcgatc gtgcgcgtcg agcagccgta tccgttcccg gccaaggaaa
     2281 tcaacggtgt gctggagaaa taccgcaacg cccggcagat tttctgggtg caggaagaaa
     2341 ccaaaaaccg cggctgctgg agcttcatgg aatcgcgcgt gcgcgaattg ctcccacaag
     2401 gcgcggcgct gggctacatc ggccgcgacg aagcggcgag ccccgcgaca ggcagccaca
     2461 agatgcacga ggtcgaggag cacgagatcc tgacgcacgc gctggacctc aagcagcgcg
     2521 ccgccgccaa acccgccgcc gcccaagccg ccgtcgccgc ggtggccacg agtgcgccag
     2581 ccaccgcgcc gtcccctatc gcggcgtcgc agtgaacggc accccgccgc ttgcggctta
     2641 gcgtctgttc cgtcccagta agaaacaaac gctaagccgc aagcggccgt agcgcgcacc
     2701 cgttcccttc caatcccccc tcgcgccacc taaaatctgc cacccttatg ccaaccaatg
     2761 tcgatatccc cgccctcggt gaatctgtgc aggaagccgt gctgctcaag tggcacaaga
     2821 acaacggcga cgtcgtcgcc gagaacgagc cgctgtgcga gctcgaaacc gacaaggcca
     2881 acgtggacat ccccgccccc gcggcgggcg tgctgcgccg cgtgaaggac gaaggcgcga
     2941 cggttcacat cggcgaaacg atcgcgagga ttgacgaatc ggccggcgcc accggtggca
     3001 atggcagccc cgcggcatcg aaaccggtga aggcggaatc ggcaccggcc gcgacgccgc
     3061 gcattaccga agcgcccaag ccaccggttg aagatctgag ccccgccgtt cgccggttgg
     3121 ttgaagagaa caagctcgat ccgaagcaga tcgccgcgac gggtcccggt ggtcggttga
     3181 ccaaggaaga tgtgctgaag cacatcgaag gcaagagcgc ggcggcgtcc gcgccgacgc
     3241 cggcagctcc ggtcgcgcca tcgccagcgc cggctcccac taaagctccg tcggaatcca
     3301 tcgtcgctcc gccgacgtcg tccaagccga tcgccggcgc gatgcaattc gacgccgccg
     3361 gtgttcatcg cgcgccgatg agcaagatgc gcgcgagcat cgctcgccgt ctggtcgaag
     3421 cccagcacac cgccgcgatc cttacgacgt tcaacgaagt cgatctgacg gaagtgatct
     3481 cgcttcgcag caagtacaag gagacgttcg aaaaaaccca cggcatcggc ctgggcttca
     3541 tgagcttctt cgcccgcgcg gtcgtgctcg gcctgcgcga gttcccccgc atcaacgcct
     3601 tcatcgacgg caacgacgtc gtctatcaca actacgtgca cctgggcatc gccgtcagca
     3661 ccgaccgcgg gctggccgtg cccgtcctgc gcaacgtgca ggacatgtcg ttcgcgaaga
     3721 tcgaaagcga aatcaaacgg ctggccgtcg ccgcccgcga cgggaaactc ggcatcgaag
     3781 aactctccgg cggcacgttc accatcacca acggcgggat cttcggttcg ctcctgagca
     3841 ccccgatcct caaccccccg cagagcggca tcctgggcat gcacgcgatc cagaaacgcg
     3901 cgatggtcgt gggcgataaa atcgagccgc gctcgatgat gtacgtcgcg ctcagctatg
     3961 accaccgctt ggtcgatggc cgcgaaagcg tgagcttcct cgtgcggctg aagcaactgc
     4021 tggaagaccc ggcgcggttg atgctcgaga tttaaagctt ctaaaaattg tagctgcgac
     4081 gcctgcgtcg cctctgcggc tcggaagaag aaggcgacgc aggcgtcgca gctacaatgc
     4141 gatgagtatc ttcagaggct tgtaagctcg ccgcccaatc acgttcgcgc ctcgaccctt
     4201 gggaccgatc acatcgcgct atccacgcgc tttggagttg atccgaaaag cccttgggat
     4261 tccccacgcg cacagaccac tcagtgcaag catcggcgcg gcgggcaatg ggacggagcg
     4321 cgggaccaca atctcggcgt ccacccctac ctccggccca ccgccggtcg gcaatgcttc
     4381 tgccgcgacg gtggccgtcg agccgttcct caacacgaga taagcaatcg gttggacgct
     4441 gagttgatcc ccgggtccga aaagggtgtc gccctgcagg cgatggccgg tgccccaggt
     4501 atcggtgatg agattgcgcg gcgcgcccgg tggatgcacc agatcgttgt cttcgctgag
     4561 ttggaacgtg atgggcgatc gggtgagcag atggctgtcg gtgccgttgt agctgccgtt
     4621 ggcgtcgctc aagctcgagc caatcggcgt ggacaaatgc gagccgtggt gtgggatcca
     4681 gtcctgcagc atttgcccgc ggatgccaag ggtggacgaa ttgccgggca cgccaaagct
     4741 gcggggctcg ccgaatgaaa tgccctcgat catctgtccc ggcggagcga cgagggcgag
     4801 caggtaaccc gtccacccct gggcggtgtc gccagaatta tcgtgggcgg gtccttgatt
     4861 tgtgacgctc aactgaccgc ccggctggac cacgccagcg tcagactgcg ctgcgaccat
     4921 cgtcagccca gcggcgcatg cgcacgcgat ggacagcacg ctcaccggct tcatggctcc
     4981 tcccttggct tagacgtgca tcggcatttg aacgtccaac cccctcgcgc tgggtgcggc
     5041 ctcagcggta gcagcttacc accgggatca actttaaggg gagaaaaaag cgcgcgaagc
     5101 aaagcccacg gatagctatc cgtgggcttt atttgcgttc ggtgcgggtc gcgcgagcat
     5161 ccggcgactc cgtcagttgt tctcaggacg cccgctgggc cacgtcacgt tgtgaatcgg
     5221 ctttaggatc tccagcacct ccgccagcag ttgctggtcg atcggttcat ccaacgccgc
     5281 gaggttcaac tccatattct tcggctccgc cgttcccacc acggttgtcg cgatgtccgg
     5341 ctgcgccacc gcgtattgca cggccagctt ctcgatgctc gcccccttcg cgcggcaatg
     5401 ggcggcggct tgggcgcaca actgcttcac ccgcggcgga gccgggtgcc agtcgggggc
     5461 gccgcgggtg ctcaacagcg ccatgctcag cggcgatgcg ctgatcacgc caacgccttt
     5521 cgccttcagc gtcgggatca gctccagcag cgatgtgtcg ttcagcgaat agtgacaata
     5581 cgagaggatt gcatcgaccg gcacgcgctc gatgacgtac cgc
//
LOCUS       DATIMH010000264         5746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4968427, whole genome shotgun sequence.
ACCESSION   DATIMH010000264 DATIMH010000000
VERSION     DATIMH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4968427"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1236)
                     /locus_tag="VLI90_04480"
     CDS             complement(184..1236)
                     /locus_tag="VLI90_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSV13490.1"
                     /translation="MTSQLDAPVKPHRVITPFRVLVTLVLVGLLAWNLGLFRRAPRVA
                     IITSGEGHYWDPIEAGAKRAADDYDVKLTVIRCKSELLPQLDAIKQATGEHYDAIAIS
                     PINPEGEATLMADVAAKTQLITLDSDSPVARRLCFVGTDNYDAGRLCGQMVRDAVPGG
                     GEVVITLGNSDKENTRLRRQGVIDELLQRPYLPERPADAYDAELKGNDFNIVATFAAG
                     ADSANATDMAVKAIKDHPNVKCLVGLLSYSGPALVKAIEQSGKARQIQVVGFDVSEDT
                     LADIEAGKIASTIMQDQFGCGYETVRLLADCAKGNSAELPVFGKKTLQCRVVKKDNVQ
                     EARQRLTGPPPATQPG"
     gene            complement(1366..2442)
                     /locus_tag="VLI90_04485"
     CDS             complement(1366..2442)
                     /locus_tag="VLI90_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV13491.1"
                     /translation="MAKLKLGVLGLGEGRSIISAALNSELWELARICDLNETLCKERA
                     EEFRFPHYTTRFDEMLADPQIDVIGIYTPDPLHAGHIRQCLDAGKHVICTKPLIDNLQ
                     DAKALLDAGTRSKRQVFVGQSSRFFEPMIRQRADFDAGKHGALFSVEAHYHADNRWFS
                     KKSWAKSGGVRWLFNGLSHPVDLVRWYVPDITDVMGYGMLTENGKAVGLEAPDAMHFV
                     MRSAGGQIARVSGAYSSPPGNHVRDSHITCVLRGDRGCTHADYSDLRYSTQFSGEGNA
                     QYDFASKEPYYFRFGGRSHHAGEYQNYIEYFARCVERGETPKPDIAEGIGTVALMSAM
                     ERSLATSQPVKVAAVLQEHGLAQM"
     gene            complement(2578..5064)
                     /locus_tag="VLI90_04490"
     CDS             complement(2578..5064)
                     /locus_tag="VLI90_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSV13492.1"
                     /translation="MSRKRINLNRDWRFHRGDIVGAAAVDFDDSNWERVGLPHTFDLP
                     YFRTAEFYVGVGWYRKRVDTRGLRLTARTFSHWLEFDGAFQVAEIFINGRRVGEHRGG
                     YTGFHCSGFEDGENMLAVRVSNEWDAKLAPRAGEHIFSGGLYRDVQMVFVPAVHVKWN
                     GVAVATENLSPDRWKVHVTTEVSNETPHPVQCTARQIIIDADGDILASFMSTQVLQSQ
                     QSTEFTQTSDAIESPRLWHPDHPNLYMVRTEVLSDDEPNDVVETMFGFRWFEWTADRG
                     FFLNGEHLYLRGVNAHQDHAGWGIGITQAACERDVRLIKEAGFNFVRGAHYPHHPAFA
                     DACDRLGLIFWSESTFWGKGGFGAEGYWNASAYPIEERDRAPFEEHCKQTLREMIRIN
                     RNHPSIVVWSMTNEAFFTYHLDRAKGLMRELVELSHQLDPTRPAAIGGAQRGGVDKIG
                     DIAGYNGDGARLFIDPGVPNMVSEYGAISKPHDAYEPFFGELQPEPFPWRAGQAIWCG
                     FDYGSIAGKQGLKGILHHNRLPKRSWYWCRNEHRQILPPPEPSDAPATQLQLSADKTT
                     IRGTDATDDCQIVISALDAGGAIARHCPPVTLTIETGPGEFPTGPSITFAEESDIPIV
                     AGQAAIAFRSYHAGQTLIRATAAGLKDATLTITTTGAPAFVEGETPRVAPRPYAPPPP
                     SAAAVAAMKNLVNVARDRPSRASSEAERHPARFANDAHAATFWEAAEEGAAAWWQVDL
                     EGFYQLSGCKLIFSAPASYRFYVEISNDGAKWTMAIDRRQSVRTDAVRNDIFDPDTVA
                     RYVRVTFSEVGSRGRAHLCELELHGVLSLR"
     gene            complement(5061..>5746)
                     /locus_tag="VLI90_04495"
     CDS             complement(5061..>5746)
                     /locus_tag="VLI90_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HSV13493.1"
                     /translation="LECFVQDDMDMPTTIALIHTSPTMIPTFKPLVAELLPAGVSTFN
                     MVDESLLCDIIREGALPPGTARRLVGHMLAAEQAGADYILVTCSSMGRAVEASRLMCA
                     AKVLRVDEPMASKAVSIGKRIGVVATLPSTLEPTVALIRSTGQAVDVQPLVVEGAFAA
                     VMGGDGAKHDALVGAAITTLAKTTDVIVLAQASMARVVDALRPEDKPVPILSSPRLAV
                     EHLASLLQR"
BASE COUNT         1020 a         1934 c         1674 g         1118 t
ORIGIN      
        1 cggcaatgcc gaggtgttca tagacgacat tgcggtgcaa cccgccaacc gatccaacga
       61 cggtcaacta caagtcgaat tcgcgccgac cgcgaagaag atcgccgtat ccgtgctgat
      121 cttcgccgcg tcatcgcccg ccggcattct gggaccggtc gagctacttg agtaatctcg
      181 cgctcacccc ggctgcgtcg ccggtggcgg tccagtcaac cgttgccgcg cttcctgcac
      241 gttgtccttc ttcaccaccc ggcactgcag cgtctttttg ccgaagaccg gcagctccgc
      301 cgaattgccc ttggcacagt cggccagcag gcgcacggtt tcatagccgc agccgaactg
      361 gtcctgcatg atcgtgctgg caatcttgcc cgcttcgatg tccgccagcg tgtcttcgct
      421 cacgtcgaag ccgacgactt gaatttgcct ggccttgccg ctctgttcga tcgccttcac
      481 aagcgccggc ccactgtacg acagtaagcc gacgaggcac ttcacgttcg gatgatcttt
      541 gatcgccttc accgccatat ccgtggcgtt cgccgagtcg gcccccgcag caaatgtcgc
      601 gacgatgttg aagtcgttgc ccttcagctc cgcgtcatac gcatcggccg ggcgctccgg
      661 cagatacggc cgctgaagca actcgtcgat cacaccctga cgccgcaggc gggtgttttc
      721 cttgtcggaa tttccaagcg tgatgaccac ctcgcccccg cccggcacgg cgtcgcgcac
      781 catctgcccg cacaagcgac cggcgtcata gttgtcggtg ccgacgaagc agagtcggcg
      841 cgccaccgga ctgtccgaat ccagcgtgat cagttgcgtc ttcgccgcga cgtccgccat
      901 cagcgtcgct tcgccttcgg gattgatggg gctgatggcg atggcatcgt aatgctcgcc
      961 cgtcgcctgc ttgatcgcgt ccagctgcgg cagcaattcg ctcttgcagc ggatgaccgt
     1021 cagcttcacg tcgtaatcgt cggccgcgcg cttggcgccg gcctcgatcg gatcccagta
     1081 atgaccttca cctgacgtga tgatcgccac ccgcggggcg cgccggaaca ggccaagatt
     1141 ccacgccagc agtccgacca ggaccagcgt gaccagcacg cggaacggcg taatgacgcg
     1201 atgaggtttc acgggcgcat ccagctgcga ggtcatgtta ttctccaatc aattcggcga
     1261 cgccgcgcga tcagcagaga tgggcggagc tgatcgcagt ggtgattgat cgagcgtaac
     1321 tcgcgcttga attggggtca aaagaattcc tattcgcgat gtagattaca tctgcgccaa
     1381 tccatgctcc tgaagcaccg ccgccacctt cacgggctgg cttgtcgcca ggctccgttc
     1441 catcgcgctc atcagcgcta ccgtgccgat gccctcggca atgtccggct tgggtgtttc
     1501 gccacgctcg acgcaccggg cgaaatattc gatgtagttc tgatattccc ctgcgtgatg
     1561 cgagcgccct ccgaagcgga agtagtaggg ttccttgctg gcgaagtcgt attgcgcgtt
     1621 gccttcgccg ctgaactgcg tgctgtaacg cagatcggaa tagtcagcat gggtgcagcc
     1681 gcgatcaccg cggagcacgc aggtgatatg gctgtcgcgc acgtgattcc ccggcgggga
     1741 actgtacgcg ccgctcaccc gcgcgatctg ccccccggct gagcgcatca cgaaatgcat
     1801 cgcatcgggc gcctccaggc cgacggcttt gccgttctcg gtcagcattc cgtatcccat
     1861 cacgtcagtg atgtccggca cgtaccatcg aacgagatcg accggatgac tgagcccgtt
     1921 gaacaaccag cgcactccac cgctcttggc ccagctcttc tttgagaacc agcggttgtc
     1981 cgcgtgatag tgcgcttcga ccgagaacaa cgccccgtgc ttccccgcgt cgaagtcggc
     2041 gcgctggcgg atcatcggtt cgaaaaaccg cgagctctgc ccgacgaaca cctgccgctt
     2101 gctgcgcgtc cccgcatcga gcagcgcctt ggcatcctgc aggttgtcga tcagcggctt
     2161 ggtgcagatg acgtgcttcc ccgcgtccag gcattggcgg atatgccccg cgtgcagcgg
     2221 gtccggcgtg tagatgccga ttacgtcgat ctgcgggtct gccagcatct cgtcgaaccg
     2281 cgtcgtgtaa tgcgggaagc ggaattcctc cgcgcgctct ttgcagagcg tctcgttcaa
     2341 atcgcagatc cgcgccagct cccacagttc gctgttcagc gccgcggaga tgatgctccg
     2401 gccttcgccc agacccagga cacccagttt cagtttcgcc attttggctc actctccgtc
     2461 gggcgcgctc aagcacccct gataaccgca ggcgaaagcc agcggggggt ttctacaacc
     2521 agcccggttc aaactcgtgc gcccccgctg gctgtcgcct gcggctatga tcaatcgcta
     2581 tcgaagcgat agcaccccgt gcagttcaag ttcgcacagg tgcgctcggc cgcgcgagcc
     2641 gacctcgctg aacgtgacgc gaacgtatcg cgccaccgta tccggatcga agatgtcgtt
     2701 gcgaaccgcg tcggtgcgca cgctctgccg gcggtcaatc gccatcgtcc acttcgcgcc
     2761 gtcgttcgaa atctcgacat agaaacgata actcgcgggt gcactgaaga tcagcttaca
     2821 gccggacagc tggtaaaaac cttcgagatc gacttgccac catgcggcgg cgccctcctc
     2881 ggccgcttcc cagaacgtcg ccgcgtgcgc gtcgttggcg aatcgcgccg gatgccgctc
     2941 cgcttcgctg ctggcccggc tgggccggtc gcgcgcgacg ttcaccaggt tcttcatcgc
     3001 cgcgaccgcc gccgccgacg gcggcggggg cgcataaggc cgcggggcga cgcgaggcgt
     3061 ttcgccttcg acgaacgcag gggcgcccgt cgtcgtgatg gtcaacgtcg catctttcaa
     3121 tcccgccgcg gttgcgcgaa tgagcgtctg ccccgcgtgg tacgagcgaa acgcgatcgc
     3181 cgcctggccg gcgacaattg gaatgtcgct ctcctccgcg aacgtgatcg atggccccgt
     3241 cggaaattcg ccggggccag tctcgatcgt caacgtcaca ggcgggcaat gtcgcgcgat
     3301 cgcgccgccg gcatccaacg cactgatgac gatttggcaa tcatccgtcg catccgtgcc
     3361 gcggatcgtc gtcttgtccg ccgacagttg cagctgcgtt gccggcgcat cgctcggttc
     3421 tggcggcggg aggatttgcc gatgctcatt tcgacaccaa taccagctgc gcttcggcag
     3481 ccggttgtga tgcaggatgc ccttcaggcc ctgcttgccc gcgatgctgc cgtagtcgaa
     3541 cccgcaccag atcgcctgtc ccgcgcgcca cgggaatggt tccggttgta gctcaccgaa
     3601 gaacggctcg tacgcatcgt gcggcttgct gatcgcacca tattccgaaa ccatgttcgg
     3661 cacgccggga tcgatgaaca gccgagcccc atcaccgttg tagcccgcga tgtcaccaat
     3721 cttgtccacc ccgccgcgtt gagccccgcc gattgccgcg gggcgtgtgg gatcgagttg
     3781 atgactcagc tcgaccagct cgcgcatcaa tcccttggct cgatccaggt gataggtgaa
     3841 gaacgcctcg ttggtcatgc tccagacaac gatcgacggg tgattgcggt tgatgcgaat
     3901 catctcgcgc agcgtctgct tacaatgctc ctcgaatggc gcgcggtcgc gttcctcgat
     3961 tggatacgca ctggcgttcc aatagccttc agcgccgaac ccgcccttgc cccagaacgt
     4021 gctttcggac cagaagatca atccgagccg atcgcacgca tctgcgaagg ccggatgatg
     4081 cgggtaatgt gcgccgcgca cgaagttgaa acccgcctcc ttgatcagcc gcacgtcgcg
     4141 ctcgcaggct gcctgcgtga tgccgatgcc ccagccggcg tgatcctggt gcgcgttgac
     4201 gccgcgcagg tacaaatgtt cgccattcag aaagaagccg cgatcggcgg tccactcgaa
     4261 ccagcggaag ccgaacattg tttccaccac gtcattcggc tcatcgtcgc tgagcacctc
     4321 ggtccgaacc atgtacagat ttggatggtc cggatgccac aggcgcggac tttcgatcgc
     4381 gtcgctggtt tgtgtgaact cggtgctttg ctgtgactga aggacctgtg tcgacatgaa
     4441 tgacgcgaga atatcgccgt cggcatcgat gatgatttgt cgcgcggtgc actgaacggg
     4501 atgtggagtc tcgttactga cttcggtcgt tacgtggacc ttccatcgat ccggcgacaa
     4561 attctccgtc gcaaccgcga cgccgttcca tttcacatgg acggcaggca cgaaaaccat
     4621 ttgtacatcg cgatacagac cgccgctgaa gatatgttca cccgcacgcg gcgccagttt
     4681 cgcgtcccat tcattgctga cgcgaacagc cagcatgttc tcgccgtctt caaacccgct
     4741 gcagtggaag ccggtgtagc caccccgatg ctcgccaacg cgccggccgt tgatgaagat
     4801 ctcggcaacc tgaaaggcgc cgtcgaattc cagccagtgc gagaaggtcc gcgcggtgag
     4861 acgaagcccc cttgtgtcca ctcgcttccg ataccacccc acgcccacgt aaaactccgc
     4921 cgtgcggaag tacggcaggt caaacgtgtg cggcagacca acacgctccc aatttgagtc
     4981 gtcaaaatcg acggccgccg cgccgacgat gtcgccgcgg tggaatcgcc agtcgcgatt
     5041 gaggttgatc cgcttgcggc tcatcgttga agcagcgacg ccagatgttc caccgccagc
     5101 cgcgggctcg acaagattgg caccggcttg tcctctggtc gcagcgcgtc cacgacgcgg
     5161 gccatggacg cctgcgccaa cacgatcacg tccgtcgtct tggccagcgt tgtgatcgcc
     5221 gcgcccacca acgcatcgtg cttcgcgccg tcgccaccca tgacggcggc aaaggcgccc
     5281 tcgaccacca gcggctgcac gtctaccgcc tggccggtcg atcgaatcag cgccaccgtc
     5341 ggttccagcg tagatggcag cgtcgcaacc acgccgatcc gtttgccgat cgacaccgcc
     5401 ttgctcgcca tcggttcgtc cacgcgcagc accttcgccg cgcacatcag tcgcgacgct
     5461 tccaccgccc ggcccatcga cgaacaggtc accaggatgt aatccgcccc cgcctgctcg
     5521 gccgccagca tgtgcccgac caaccgccgc gccgtccccg gcggcaaggc gccttcgcga
     5581 atgatgtcgc acagcaggct ctcatcgacc atgttgaacg tgctgacgcc cgccgggagc
     5641 agctcagcaa cgagcggctt aaacgtcggg atcatcgtcg gcgatgtgtg gatcaacgcg
     5701 atagttgtgg gcatatccat gtcatcctga acgaagcatt ctagcc
//
LOCUS       DATIMH010000265         4262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2656950, whole genome shotgun sequence.
ACCESSION   DATIMH010000265 DATIMH010000000
VERSION     DATIMH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2656950"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VLI90_04500"
     CDS             complement(<1..732)
                     /locus_tag="VLI90_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13494.1"
                     /translation="MTEINLPTSSAGPPESSSASAERTPGADEPAGTSFASRVLVDVL
                     ARASAKIGLLWIILLVVCAVFAPLLANSHPLLMRSGGRWSSPMLRHLSPADSVLQIGF
                     WAGAVLVIVPGLSRKARWWSWSAVLVLAMIICPLTVHPPQLVVYEAYRLDQQAGKIER
                     ALYAPIAFSPDDHLRDQPDARLTAPSSTHWGGTTSDSADLLSNLIYACRIALSIGFIS
                     TGIALILGIIIGGLMGYYVGWVDLLG"
     gene            complement(855..1928)
                     /locus_tag="VLI90_04505"
     CDS             complement(855..1928)
                     /locus_tag="VLI90_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV13495.1"
                     /translation="MLTYIVRRFLLMFPTLLGITILVFFVMAYSPGGIGGPLLDRTGN
                     MKSADAARVREYYEKRFGINNPPLIQYFRWLNLISPFGKQQNEDGSFGKLGFKWPSLG
                     ESMQQHRPVMDLIAESLPITLLLNLISIPLIYGTGIFTGIVAARHRGSLYDTATGITQ
                     LATWSVPTIWAGVMLIGLLANRQYFKWFPTSGLHDADALLMPFFPHHLPTGWARGWLL
                     DTIWHLVLPVICLSYGGSAFLMKLTRGSVLENLGADYVRTARAKGVDENVVLYRHVFR
                     NSLLSLITVAANILPALLGGSVIVESIFSVPGMGRLGVESVQFRDREVVLAVTLIGGL
                     VGLLSQILRDILYALADPRVSYD"
     gene            complement(1976..3832)
                     /locus_tag="VLI90_04510"
     CDS             complement(1976..3832)
                     /locus_tag="VLI90_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSV13496.1"
                     /translation="MENRFGIKDFFLFLVLFVLIGLVVLAMVQYDRQWELQRQTNALL
                     TQQTTDLSRIRRLLEQGVSFNSNGGAPTTQSASSMTAGFERVLAAHAAPDFAAGGQLL
                     LMSQATTARLTPLIASDAFAFDVWGYVMDSLVNRDPDTLQWLPSLAQSWKISDDSLTI
                     DFQLRRGITFSDGSPFSADDVVFTFDFMRNPQVEDPIMRIGAERLDHVEKTGDYSVRF
                     VFKEPYYKSFETAGGTPIMSKAFYAKYSPTDFNNSTGLLIGSGPYRLPDPTSWRPQPG
                     QPIVLVRNERYWGPTPSFDRIVWNVIEQPSARVTAFRNGDIDFMGGQGPPTPEQFTQL
                     IADQQLAPHINHWALTSPAEAWYYLGWNEKQGLNGKPSAFADPRVRRAMTMLTDRDAI
                     LKNIIHGYGAILSSPFPPNTPQCDPSIQPWPYDPVAAEKLLAEAGFHRTGDRMIGPAG
                     QPLTFKLLFNSNSEPRRRIASLLHDAYAKLGIDAQSESAEWSVFEQRLKDRQYDCVIG
                     AWGGALEGDPYEELHSSQIEHTGENFIQFNNPDVDKVIEQARATVDEAKRMPMWHKLH
                     QLIHDQQPYTYLFIYRELDIARDRIHGMKPTKVLGLNPLGEWYIPQALQRAQ"
     gene            complement(3898..>4262)
                     /locus_tag="VLI90_04515"
     CDS             complement(3898..>4262)
                     /locus_tag="VLI90_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSV13497.1"
                     /translation="RGLPFIEAARAAGVPERRIFLRHIFPNLAGPIIVYATLTVPRAM
                     LEESLLSFLGIGIAPPLSTWGSLASDALQPALNPVNPGWWLLLFPCTLLAVTLLSLNF
                     LGDGLRDLFDPKSGDTGY"
BASE COUNT          835 a         1346 c         1341 g          740 t
ORIGIN      
        1 gccgagcagg tccacccatc cgacgtagta gcccatcaag ccgccgatga tgatgccgag
       61 gatcagcgcg atgccggtcg agatgaagcc aatcgacagc gcgatgcggc aggcataaat
      121 caggttgctc agcaggtcgg ccgagtcgga ggttgtgccc ccccagtgcg tgctgctggg
      181 cgctgtgagc cgggcgtccg gttgatcgcg caagtgatcg tccggcgaaa acgcgatcgg
      241 cgcgtacagc gcccgttcga tcttcccggc ctgctgatcc agtcgatacg cctcatacac
      301 aaccagctgc ggcggatgca ccgtcagggg gcagatgatc atcgccagca ccagcaccgc
      361 cgaccaactc caccatctgg cttttcgcga cagccccggc acgataacca gaacggcgcc
      421 ggcccagaag ccgatctgca gcacactatc ggccggcgag aggtgacgga gcatggggct
      481 ggaccagcgc ccgccgctgc gcatcagcag cggatggctg ttggccagca gcggcgcaaa
      541 gaccgcgcat acgaccagca ggatgatcca gagcaatccg atcttcgcgg acgcgcgtgc
      601 caacacgtcc accagcacgc gactggcgaa ggaagtgccg gcgggttcgt ccgcgccggg
      661 cgtccgctcg gcagatgccg atgacgactc cggcgggccg gcgctgctgg ttggaaggtt
      721 gatctcagtc atcgatccct atcgttcgca cgctgcgcga cgaccgggct tgccgcttta
      781 acggtaagcg ttacccttcg caacgctgct taccgctaat gcggtaaacc cggtcgaagc
      841 aatgcgcatc aacctcagtc atagctcacg cgcgggtccg ccaacgcgta aaggatgtcg
      901 cgcaggattt ggctcagcag gccgactagg ccgccgatca gcgtcaccgc gagcacgact
      961 tcgcgatcgc ggaactgcac cgactccacg cccaaccggc ccatgccggg gacgctgaag
     1021 atgctctcga cgatgaccga tccacccagc agcgccggca ggatgttggc cgctaccgtg
     1081 atcagcgata gcaggctgtt tcgaaagacg tgccggtaca atacgacgtt ctcgtcaaca
     1141 cccttggccc gcgcggtgcg gacgtagtcg gcgccgagat tctccagcac cgacccgcgc
     1201 gtcagcttca tcaggaacgc gctgccgccg taactcaggc agatgaccgg caacaccaag
     1261 tgccagatcg tgtccagcaa ccagccgcgc gcccagccgg tgggcagatg gtgcgggaag
     1321 aacggcatca gcagcgcatc ggcatcgtgc aggccggagg tgggaaacca cttgaaatat
     1381 tgccggttgg ccagcaggcc aatcagcatg acaccagccc agatggtcgg caccgaccac
     1441 gtcgccagct gtgtgatgcc ggtcgcggtg tcgtacaggc ttccgcgatg gcgcgccgcg
     1501 acgatccccg tgaagatgcc cgtgccatag atcagcggga tactgatcaa gttgagaagc
     1561 agcgtgatcg gcagcgattc ggcgatcaaa tccatcaccg ggcgatgttg ctgcatgctc
     1621 tcgccgaggc tcggccactt gaaacccagc ttgccgaagg agccgtcttc gttctgttgt
     1681 ttgccgaacg gagagatgag gttcaaccat cggaagtatt gaatcagcgg cgggttgttg
     1741 atgccgaatc gcttctcgta atactcgcgc acgcgggcgg cgtcggcgct tttcatatta
     1801 cccgtgcggt ccagcagcgg cccgccaatc ccaccgggtg aatacgccat cacgaagaac
     1861 acgaggatcg tgatccccaa tagcgtggga aacatcagca ggaatcgtcg gacgatgtac
     1921 gtcagcattc agcagacctt aatcgcgctc gcgtgcgcac gctcgcgccc cggaactact
     1981 gcgcccgctg cagcgcctgc gggatatacc attcccccag cgggttcagg cccagcacct
     2041 tcgtcggttt catgccgtga atgcgatcgc gcgcgatgtc caattcccga tagatgaaca
     2101 gatacgtgta cggctgctgg tcgtgaatga gctggtgcag cttgtgccac atcggcatgc
     2161 gcttcgcctc atccaccgta gcgcgggcct gttcgatcac cttgtccacg tcgggattgt
     2221 tgaactggat gaagttttca ccggtgtgtt cgatctggct cgaatgcagc tcttcatacg
     2281 ggtcgccctc cagcgccccg ccccaggcgc cgatcacgca atcgtattgc cggtccttca
     2341 agcgctgctc gaagaccgac cactccgccg actccgactg cgcgtcgatg ccgagcttcg
     2401 cataagcatc gtgcagcagc gacgcgatcc gccggcgcgg ctcgctgttg ctgttgaaca
     2461 gcagcttgaa ggtcagcggc tgaccagcgg gcccgatcat gcggtcgccg gtgcgatgaa
     2521 agcctgcctc ggccaacagc ttctccgccg caaccggatc atacggccac ggctggatcg
     2581 acgggtcgca ctgcggcgtg ttcggtggga acgggctgct gaggatcgcg ccgtacccgt
     2641 ggatgatgtt cttcaggatc gcatcgcgat cggtcagcat cgtcatcgcc cgccgcaccc
     2701 gcgggtccgc gaacgcgctg ggcttgccgt tcaagccctg cttctcattc caccccagat
     2761 aataccacgc ctccgcggga gatgtgaggg cccagtggtt gatgtgcggc gcgagctgct
     2821 ggtccgcgat caactgcgtg aactgctccg gcgtcggtgg gccttggccg cccatgaagt
     2881 cgatgtcacc gttgcgaaat gccgtcacgc gcgctgacgg ttgctcgatc acgttccaca
     2941 caatgcgatc gaacgacggc gtgggtcccc aataccgttc gttgcgcacg agcacgatcg
     3001 gctggcccgg ttgcggccgc caagatgtgg gatcaggcag ccgatacggc cccgagccga
     3061 tcaacagccc cgtcgagttg ttgaagtccg tcggcgaata cttcgcgtag aacgccttgc
     3121 tcatgatcgg cgtgccaccg gcggtttcga agcttttgta atatggctcc ttgaagacga
     3181 accgcacgga gtaatcgccg gtcttctcga catggtcgag ccgctcggcc ccgatccgca
     3241 tgatggggtc ttccacctgc ggattgcgca tgaagtcgaa ggtgaacacg acatcatcgg
     3301 ccgagaatgg cgagccatct gagaacgtga tgccgcggcg aagctggaag tcgatggtga
     3361 gcgagtcgtc ggaaatcttc cacgattgtg cgaggctggg cagccattgc agcgtgtctg
     3421 gatcgcggtt caccagcgaa tccatgacgt agccccacac gtcaaacgcg aaggcatcgc
     3481 tggcgatcag cggcgtcaat cgcgcggtgg tggcttggct catcaacaac aactgcccgc
     3541 ccgcggcgaa atcgggcgcc gcgtgtgccg ccagcacgcg ctcgaagccg gccgtcatcg
     3601 atgaagcaga ttgagtcgtc ggcgcgccgc cgttggaatt gaacgacaca ccctgctcga
     3661 gcagccggcg gatgcgcgac aggtccgttg tctgctgcgt gagcagcgcg ttggtttgcc
     3721 gctgaagctc ccactgccgg tcgtactgca ccatcgctag gacgaccagg ccgatcagca
     3781 caaataagac gaggaagaga aagaagtcct tgatgccgaa gcggttttcc atgggttttg
     3841 tagcatgggc tagcagcccg tgacgaggcg gcggtgcttg gacacgacac gcacgggcta
     3901 gtagcccgtg tcacctgatt tcgggtcgaa caggtcgcgc aaaccgtcgc cgaggaagtt
     3961 caggctcagc agcgtgacgg cgagcaaggt acacggaaac agcagcagcc accagcccgg
     4021 attgaccggg ttgagcgccg gttgcaacgc gtcggacgcc agcgaacccc aggtgctcag
     4081 cggcggcgcg atgccgatgc cgaggaagct gagcagcgat tcttccaaca tcgcgcgcgg
     4141 caccgtgagc gtcgcgtaca cgatgatcgg cccggccaag tttggaaaaa tgtgccgcaa
     4201 gaagatgcgg cgctcgggaa cgccggcggc gcgggcggct tcgatgaacg gcaggccgcg
     4261 aa
//
LOCUS       DATIMH010000266         4778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2657132, whole genome shotgun sequence.
ACCESSION   DATIMH010000266 DATIMH010000000
VERSION     DATIMH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657132"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VLI90_04520"
     CDS             <1..1010
                     /locus_tag="VLI90_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13498.1"
                     /translation="EINIVMVLRDEASGIYVWSESFRLGLGNWFDAQQRIIRRIATSL
                     NVQLSAERLMRLAGEPDVSLDLHDRWLRGQSLHFKYDAESWQRAVTIFRDAIRENPTY
                     SPCYSSLVQMNNVEHFIHPGIFRDPDKARTTLELAKCAVQLDPVDSRAHLCCGWSYVM
                     VHREIEAAAHMDLACELNENDPWTLVSGALYRAFCGSIEQARVLAEQSLAQSPAPTNL
                     GWAYHCIIRFIGSDFVGALEAFDRSQGVVPILPAWRAAALFHIGEVEKAREEAARFVN
                     GLRSFWVGSSSPTDEAVARWVLQAHPISVRERWESLRLGLRGAGLPVDGIAYQAISAS
                     N"
     gene            complement(1007..1402)
                     /locus_tag="VLI90_04525"
     CDS             complement(1007..1402)
                     /locus_tag="VLI90_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13499.1"
                     /translation="MQVTNHERWGNLIKTWSTGKNYLDDDNEYPIPATIDEFKEQLAK
                     AQVFMTVPDRYTKVNFVEQHMDTIVVRLPPAFAIADSEEKLSQPGATYPLPPFYKRLF
                     NGLDPVIPEADKFKVHAERIGDYTISLCS"
     gene            complement(1461..3248)
                     /locus_tag="VLI90_04530"
     CDS             complement(1461..3248)
                     /locus_tag="VLI90_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13500.1"
                     /translation="MLSRLAIEPTSEILSMQVQNVAEASTLQTSGTEARDAVQAAAAP
                     GFRHFLHTPLPSQRFRAFGIDPLESPALQPRLRGLMHRLSRRMDAEIAWPWPDDEAAV
                     ERWENPGIPSGYTYLLQFVAHDLVHSAIPLSVAGTLGAGTANARRAPLRLETLFGSGP
                     VGSPHIYALDAPNDDRRTKLRLGRMRWKENEPATGCPFRDIARTGAENVTGVDRSIAG
                     MRVALTEALVADPRNDDHAVISQLTALFAVLHNSLIDLIRRQEGDRGPNRRFGAAYKR
                     FLCAREALTLIYHNILRNDLMRRISHPAIFAAYSGPAPHFIDRPDPPGSGEWQIPFEF
                     SHGAFRFGHAMVRNEYCINDRSLHDLNNTLEKTSANDPANMPLDETWMVQWSRFFEIN
                     ETRPNFSRRIGPFLSDGLGNEQIFPAFDETQRIGLLYRDLFGAGIAGMWSVDALIAEI
                     GGRRPHFIAKSRLLADRSYRVSQLREWLAAAPAYGALTADDIETLSNDPPLPFFILFE
                     AMRQPGAEGLRLGLLGSIIVSEVIFGVLACDPRSSSGDALRGQLADLSSEYYPANFFE
                     DIPDISSMAQLVEFTTEIAKLRQAVPAFL"
     gene            3364..>4778
                     /locus_tag="VLI90_04535"
     CDS             3364..>4778
                     /locus_tag="VLI90_04535"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HSV13501.1"
                     /translation="MSRSLSDELFDASAGTFAYGGITAATMIPRAMSKEFGVNPIAIP
                     QVLQRLAAAFALTFSISAVACLLSSAACAEPVFSFEGTPGKLPKTVVPIHYAIELKPD
                     TTSLALPGVEIIDIEVHAPTARLTLNAVNTTFASVTVDDGTGRADIALDASAETATFT
                     FAQPLGVGPHKLRVEFAARINKFDRGFFFVDYPTDNGVKRLLTSKLEPSDARRIFPCW
                     DEPAFKASFALTVAVPRNFLAVGNMPVVREAPLEPNLKQVAFATTPDMSTYLFVLTVG
                     ELERITAEIDGVTVGVVATAGKAAKGQFALDSGVKLLSWFNDYFGVKYPLPKLDLIAV
                     PGGFGGAMENWGGITFFESRLLFDPATNPDSARRGIFGIIAHEMAHQWFGDLVTMAWW
                     DNLWLNEGFATWMATKASEQFYPQWQSWLNGYAQKQLALALDARRTSHPIQQPIADES
                     EAMVAFDAITYNKGQALIRML"
BASE COUNT          884 a         1435 c         1511 g          948 t
ORIGIN      
        1 ccgaaatcaa catcgtgatg gtgctgcggg acgaagcgtc cggaatctac gtgtggagcg
       61 aaagttttcg tctcggtctc ggcaactggt ttgacgccca gcaacggatc atccgccgga
      121 tcgcaacttc gctgaacgtg cagttgtcgg ccgagcggct gatgcggctg gcgggtgagc
      181 ccgacgtttc gctcgatctg catgaccgct ggctgcgcgg ccagagcctg cacttcaaat
      241 acgatgcgga aagctggcag cgcgcggtca ccatcttccg cgatgcgatc cgggaaaatc
      301 ccacctactc gccttgctac agcagcctcg tgcagatgaa taatgtcgag catttcattc
      361 acccgggcat cttccgtgat ccagataagg caaggacaac gttggagctg gcgaagtgtg
      421 ccgttcaact ggatccggtc gattcccggg cgcacctgtg ctgtggctgg tcctacgtga
      481 tggtgcatcg ggagatcgaa gcggcggcgc acatggacct tgcttgcgag ctaaacgaaa
      541 acgatccttg gaccctggtg tccggcgccc tctatcgtgc cttctgcgga tcaattgagc
      601 aggcccgtgt gctggccgaa caatcgctcg cgcagtcgcc tgcgcctaca aatctcgggt
      661 gggcttacca ttgcatcatt cgcttcatcg gcagcgactt tgttggcgcg ctcgaagcct
      721 ttgatcgttc ccagggtgtc gtgccgatcc tgccagcatg gcgggctgca gcgcttttcc
      781 atatcggaga ggtggaaaag gcgcgagagg aagcggcgcg gttcgtcaat ggactgcgtt
      841 cgttttgggt cggctcatcc tcgccgaccg acgaggctgt cgcgcggtgg gtgctgcaag
      901 ctcatccgat cagtgtgcgc gagcgttggg agtccctgcg cctcggtttg cgtggcgcag
      961 gacttcccgt tgatggtatc gcctaccagg cgataagcgc gagcaattag gagcagaggc
     1021 tgatcgtgta gtcgccgatg cgttcagcat gcaccttgaa cttgtcggct tccggaatca
     1081 ccggatccag accattgaac aaccgcttgt agaacggcgg aagcggatag gtcgcgccgg
     1141 gttggctcag cttctcctcg gaatccgcga tcgcgaaggc gggcggcaac cgaacgacga
     1201 tcgtatccat gtgctgctca acgaagttca ccttggtata acggtccggc accgtcatga
     1261 acacctgcgc cttggcgagt tgctccttga attcgtcgat cgtggccggg atcggatatt
     1321 cgttgtcgtc atccagatag ttcttgcccg tcgaccaggt cttgatcagg ttgccccagc
     1381 gctcatgatt ggtaacttgc atcctctcga gtgccatctt acttactcct ccgggttgcg
     1441 tgagacggaa cgatttccgc tcacaaaaat gcgggcacgg cttgccgcag cttggcaatt
     1501 tcggtggtga actccaccag ctgcgccatg ctcgagatgt cgggaatgtc ctcgaaaaaa
     1561 ttcgccggat aatattcgct ggacaaatcg gcaagctggc cgcgaagcgc gtcgccacta
     1621 gatgagcgtg gatcgcaggc gagcacgccg aagatcactt ccgatacgat gatcgaaccc
     1681 agcagcccga gacgcagccc ttccgcgccg ggttgccgca tcgcctcgaa caggatgaag
     1741 aacggcagcg gcggatcgtt cgacaacgtc tcgatgtcgt cggccgtgag cgccccatag
     1801 gccggggctg cggccagcca ttcgcggagc tggctgacgc gatagctgcg gtcggcgagc
     1861 aggcgcgact tcgcgatgaa gtgcggtcgt cgtccgccga tctcggcgat cagggcgtcc
     1921 accgaccaca tgccggctat accggcacca aacaggtcac gatagagcag gccgatgcgc
     1981 tgggtctcgt cgaacgccgg aaagatctgc tcgttgccga ggccgtcgct gaggaaaggt
     2041 ccgatgcgtc ggctaaaatt cggccttgtt tcgttgatct cgaagaaacg cgaccattgt
     2101 accatccatg tctcgtcgag tggcatgttg gctggatcgt tcgctgaggt cttttccagt
     2161 gtgttgttaa ggtcgtggag cgaccggtca ttgatgcagt attcgtttcg caccatcgcg
     2221 tgaccgaacc gaaaggcgcc gtgcgagaac tcgaagggaa tctgccattc gccgcttccg
     2281 ggtggatcgg gccggtcgat gaaatgtggc gccggtccgc tataggcggc aaagatcgcg
     2341 gggtgactga ttcggcgcat cagatcgttg cgcaggatgt tgtgatagat cagcgtcagc
     2401 gcctcacgcg cgcatagaaa gcgcttgtaa gccgccccaa agcgtctgtt cggcccacgg
     2461 tcgccctctt gacgtcgaat gagatcgatc aggctgttgt gcagcaccgc gaacagcgcc
     2521 gtcagttgcg atatcacggc gtggtcgtcg ttgcgcggat cggcgaccag cgcttcggtg
     2581 agggcgacgc gcatgccggc gatgctgcga tcgacgccgg tgacgttctc ggcgccggtt
     2641 ctcgcgatat cgcggaacgg acaaccggtc gccggctcgt tctccttcca gcgcatccgc
     2701 cctagccgca gcttggtgcg ccggtcgtcg ttcggcgcgt cgagcgcata gatatgcggg
     2761 gaaccgaccg gaccgctgcc gaagagggtt tccagccgca gcggcgcgcg acgcgcattg
     2821 gcggtgccgg cgccgagcgt tccggccacc gaaagcggga tcgccgaatg gaccaggtcg
     2881 tgcgcaacga attgcaggag gtaagtgtaa cccgaaggga tgccggggtt ttcccaacgc
     2941 tcgaccgcag cttcgtcgtc gggccacggc caggcaattt cggcatccat ccgccgcgac
     3001 aggcggtgca tcagcccgcg cagccgtggc tgcagcgcgg gcgattccag cgggtcgatc
     3061 ccgaacgcgc gaaaccgctg cgaaggcagc ggcgtatgca ggaaatgacg aaagccggga
     3121 gcggctgcag cttgcacagc gtcgcgcgct tctgttccag aagtctggag agtggaagct
     3181 tcagcgacgt tctgtacctg catcgacagt atctcgctcg ttggctcgat cgcaagcctg
     3241 ctgagcaagt cgtgaaatta tagtgagcta aatcattaag ggttgtggac aggtgtggac
     3301 atcttttgcg tgcgcaccgt ttgagcgcgc gtggtgcgtg gtcttttccg ccacaacatc
     3361 tggatgtcac gatccctgtc cgacgagctg tttgatgctt cggcaggcac ttttgcctat
     3421 ggcggaataa cggccgcgac tatgataccg cgcgcaatgt cgaaggagtt tggcgtgaac
     3481 ccgatcgcga taccgcaggt gctgcaacgg ctcgccgcag cgttcgcgct gacgttctcg
     3541 atatccgccg tcgcttgctt gctatccagc gccgcgtgcg ccgagccggt tttttccttc
     3601 gagggaacgc cgggaaagct gccgaagacc gtcgttccga tccactatgc gatcgagctc
     3661 aaacccgaca ccacaagcct cgcgctgccg ggcgtggaaa tcatcgacat cgaggtgcac
     3721 gcgcccaccg cgcggctgac gctcaacgcc gtcaacacca cgttcgcctc cgtcaccgtc
     3781 gacgacggca cggggcgcgc cgacatcgcg ctcgacgcca gcgccgagac cgccacgttc
     3841 accttcgcgc aaccgctcgg cgttggcccg cacaagcttc gtgtcgaatt cgcggcgcgg
     3901 atcaacaaat tcgatcgcgg cttcttcttc gtcgattatc caaccgataa cggcgtcaag
     3961 cgcctgctca ccagcaaact cgaaccgtcg gacgcgcggc ggatctttcc gtgctgggac
     4021 gagcccgcct tcaaggcaag tttcgcgttg acggtcgcgg tgccgcggaa tttcctcgcg
     4081 gtcggcaaca tgccggtggt gcgtgaagcg ccgctggagc cgaacttgaa gcaggtcgcg
     4141 ttcgcgacca cgcctgatat gtcgacctac ctgttcgtgc tgacggtggg tgagctggag
     4201 cggatcacgg cggagatcga cggcgtcacg gtcggcgtcg tcgccaccgc cggcaaggcg
     4261 gcgaagggac agttcgcgct cgatagcggc gtcaagctgc tgtcctggtt caacgactac
     4321 ttcggcgtca aatatccatt gcccaagctc gatctgatcg cggtacccgg cggcttcggc
     4381 ggcgcgatgg agaactgggg cggcatcacc tttttcgaga gccggctgct gttcgatccc
     4441 gcgaccaatc cggacagcgc gcgccgcggc atcttcggca tcatcgccca tgaaatggcg
     4501 catcaatggt tcggcgatct cgtcaccatg gcgtggtggg acaatctgtg gctcaacgag
     4561 ggctttgcga cctggatggc aaccaaggcc tcggagcagt tttacccgca atggcagagt
     4621 tggctgaacg gctacgccca gaagcaactg gctttggcgc tcgacgcgcg gcggacctcg
     4681 catccgatcc agcagccgat agccgacgag agcgaggcga tggtcgcgtt cgacgccatc
     4741 acctacaaca agggtcaggc gctgatccgc atgctgga
//
LOCUS       DATIMH010000267         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_348201, whole genome shotgun sequence.
ACCESSION   DATIMH010000267 DATIMH010000000
VERSION     DATIMH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348201"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VLI90_04540"
     CDS             <1..780
                     /locus_tag="VLI90_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13502.1"
                     /translation="AGAQTSLTNVLATGAMRAINARSANLLGQVNVTGSLNSLSLNFV
                     QAGNITVGGGVGNGALAVNLGRALDTSITSAIPISSLTAAAYLNTDNVPDLITAPAIG
                     TIRVKGTFGGTQVVTASVASVIVGGSIQGSSVLANDRIGVVAALNGISDSTFFAGIRT
                     DLTGALPASTDDFVNKSAAIGRVYVRSGGFSNSLVAGWHVGALSLGNVQTVDGGSTFG
                     VSGDRVAAVVGAFTDASPIHAVELDDPTRSLVQSNFVVRSI"
     gene            complement(1232..2734)
                     /locus_tag="VLI90_04545"
     CDS             complement(1232..2734)
                     /locus_tag="VLI90_04545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV13503.1"
                     /translation="MTDGSVQQQSFTAGRPTGPALRRQLRLITLAWVFGSVWMTATAG
                     APLTLFAKGLGANEFQFGILAALPYLAAFLSLLGTVLTERVGHRKAFFLATLYLQRFA
                     WFPIALVPIWLMHRGFPASGVPLLAFLALIFLMNAGGAVGGVPWTSWMADLVPPRVRG
                     KYFSRRRQWAVLSAIPAAIFVGWVLDHHIDSRNQSALMSTCAMLFMFAAVFGTADIAT
                     FHLVPDIPKTPRRGGAFWRALLEPLRNRSFLWATGFIALMTFAMTIMNQFVTLYLLER
                     VHATSTQTQLMVLIAPAIAQLLTLGVWGAAADRMGKKPLLILAGIGMVLPGLGWSLIT
                     PTSVWIGYAAALIGTAFWTGIEIANFNLVIELSGSDATHDGNAKGSSSYVAVNSIVIN
                     TAGLLGGLASGVIAQLLVHWHAHPRGAFKTFDYFDVLFAISALLRLAAVVIFLPNIHE
                     PSARPSVETLRFMTSNVYNNLVGAAMQPIRILRRRARAETAETQQGPTLS"
BASE COUNT          584 a          994 c         1022 g          538 t
ORIGIN      
        1 gcgggcgcgc agacctcgct gaccaacgtg ctggccaccg gcgcgatgag ggccatcaac
       61 gcccgcagcg ctaatctgct ggggcaggtc aacgtcaccg gaagcctcaa cagcctgagc
      121 ctgaacttcg tgcaggccgg gaacatcacc gtcggcggcg gggtcggcaa cggcgcgctg
      181 gccgtcaacc tcggacgcgc gctcgacacc agcatcacct cggctattcc gatctcgtcg
      241 ctcaccgcgg cggcgtacct caacaccgac aacgtgcccg acctgatcac cgccccggcc
      301 atcggcacga tccgcgtgaa gggcacgttc ggcggtacgc aggtggtcac cgcgagcgtc
      361 gcctccgtca tcgtcggcgg gtcgatccag gggtcgtcgg tactggccaa cgaccgcatc
      421 ggtgtcgtcg cggcgctgaa tggcatcagc gacagcacgt tcttcgccgg catccgcacc
      481 gacctgaccg gcgcgctgcc ggcatcgacg gatgacttcg tgaacaagtc cgccgccatc
      541 ggccgcgtct acgtgcgcag cggtgggttc agcaattcgc tcgtcgccgg ctggcacgtc
      601 ggcgcgctga gcctcggcaa cgttcaaaca gtggacggcg gatccacctt cggcgtcagc
      661 ggcgaccgcg tcgccgccgt cgtcggcgcg ttcacggatg cttctccaat ccacgcagtc
      721 gaattagacg atcccacgcg ctcgctggtg cagtcgaact tcgtggtacg gtcgatctaa
      781 cgctgtcgaa cgcagattga tgaatgcaga atgaagagga gcgaaagctt ttctcattct
      841 gcattctggc ttctcggcag aacgtgggcg ccgctgtgcg catgcctttg cgtccttccg
      901 cgcgcgattg atcaatccgc aggcggcgcg acattcccgt ttcgcgcaag caaaacaaat
      961 gcaaccgccg agggcgccga gcacgccgag ataaacgccg agaaggcatt cagagaagag
     1021 gtttaaccac agatttcaca gatgacacag attcagaagg agcattcttc aatctgtgta
     1081 agactgtgaa atctgtggtt aaaattcttc ctcctctcgg cgtcttcctc ggcgtgctcg
     1141 gcgccctcgg cggttgcatt gaatcccgaa cgagtgagcc gacatataaa acaaaccggg
     1201 cgtgggtgcc gggtgcgggg tccggcttct ctcaggagag cgtcgggcct tgttgtgttt
     1261 cggccgtctc cgctctcgcc cgccttcgca gaatgcgaat cggctgcatc gcggcgccga
     1321 cgaggttgtt gtagacgttg ctggtcatga agcgcagcgt ctcgacgctg ggccgggcgc
     1381 tgggctcgtg gatgttcggc aggaagatga ccaccgccgc caggcggagc agggcgctga
     1441 tggcgaagag cacgtcgaag taatcgaacg tcttgaacgc gccgcgcgga tgcgcgtgcc
     1501 agtgcaccag caattgcgcg atcacgccgg aggccagccc gccgagtaat ccggcggtgt
     1561 tgatgacgat cgagttcacc gccacataac tgctggagcc tttggcgttg ccatcgtgcg
     1621 tcgcgtcgga gccggacaac tcgatgacca ggttgaagtt cgcgatctcg atccctgtcc
     1681 agaacgcggt gccgatcagg gcggcggcgt agccgatcca aacgctcgtc ggcgtgatca
     1741 gcgaccagcc cagtcccggc aggaccatgc cgatgccggc gaggatcagc agtggctttt
     1801 tccccatgcg gtcggccgcc gcgccccaga cgccgagcgt gagcagctgc gcgatggccg
     1861 gtgcaatcag caccatcagc tgggtttgtg tgctggtcgc gtgcacgcgt tccagcagat
     1921 agagcgtgac gaactggttc atgatcgtca tcgcgaacgt catcagcgcg atgaacccgg
     1981 tggcccagag gaacgagcga ttacgcagcg gttcgagcag cgcccgccag aacgccccgc
     2041 cgcgccgcgg cgttttgggg atgtcgggca ccaggtggaa cgtcgcgatg tccgccgtgc
     2101 cgaagacggc ggcgaacatg aacagcatcg cacaggtgct catcagcgcg ctttgattgc
     2161 gagaatcaat gtgatgatcg agaacccaac caacgaagat ggccgcggga atggcgctga
     2221 gcaccgccca ttgccggcgg cgcgaaaagt atttgccgcg cacgcgcggc gggacgaggt
     2281 ccgccatcca gctggtccac ggcaccccgc cgacggcgcc accggcgttc atcaaaaaga
     2341 tcaacgcgag aaaggccagc agcggcacgc ctgatgcggg gaagccgcgg tgcatcagcc
     2401 agatcggcac gagcgcgatc gggaaccacg cgaagcgttg caggtaaagc gtggcgagga
     2461 agaacgcctt gcgatgcccg acgcgttcgg tgaggacggt gccgagcagc gacaggaacg
     2521 cggcgagata tggcagcgcc gccaggatgc cgaactgaaa ttcgttcgcg ccgagtccct
     2581 tggcgaagag cgtcagcggc gcgcccgcgg tggcggtcat ccagacggag ccgaataccc
     2641 acgcgagcgt gatcaggcgc aactgccggc gcagcgccgg gccggtcggc cggccggctg
     2701 tgaacgactg ctgctgcacc gatccgtctg tcaacgctgt cccatccatg gtagctcaat
     2761 cgtgggatcg taagggtgag cacgtttgcc gcaagcgtcg cccgcgcggt ggtgatgctt
     2821 gattccaatc gcgcccgcgc agtcgccctc gacgccggtg tctgaggtac tccaaaggct
     2881 cgggttcttc cgcgcatgga cccgggtctt cgcgagtacg cttagagacc ggcgtcgggg
     2941 gcggtccacg aaacttgact cgcgcccgcg ggtacagcac caacgaatcg ctttggacat
     3001 tgtcctgttc cttgcccgat gcctatgttc ggcgcgtcag acaaggagtt gtgagtggtc
     3061 cgtttccctc agttcattct cgctgcagca attctcagcc atgccgcgct ggcggcgacg
     3121 acggtgccgg cgacgacg
//
LOCUS       DATIMH010000268         4702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4100391, whole genome shotgun sequence.
ACCESSION   DATIMH010000268 DATIMH010000000
VERSION     DATIMH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4100391"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VLI90_04550"
     CDS             complement(<1..296)
                     /locus_tag="VLI90_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13504.1"
                     /translation="MLISAIVGVGLRSGVALATANPATWNGNSGTSSSWGDVANWNGT
                     NGNPDGGGTGVVHFAGSNRLTPNNDYGAFTQFNQIQFDSGASSFQLNGNVIKLFG"
     gene            806..2770
                     /locus_tag="VLI90_04555"
     CDS             806..2770
                     /locus_tag="VLI90_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HSV13505.1"
                     /translation="MPQQPASGTASVAPVIKQALDRRKSIVSLSNVTIRFAGDSGDGM
                     QVAGTQFSDVSALVGNDISTLPDFPAEIRAPAGTLAGVSGYQIHFADSDIFTPGDDVD
                     TLIAMNPAALKMNLRSVRPNGNVIVNEDAFTAADLRKAGYETNPIEGNTIEGFRLIKV
                     PIERLNAEALKDSGLTAKEIDRCKNFFALGLVCWLYDRPLEPTLKYIRAKFGKKQANV
                     AAANETVLKAGHAFGETAELFPAQYRVEKAKLPPGRYRKITGNEAVALGMVTASRLAG
                     KQLFYASYPITPASDILHSLAAMKQFGVVTFQAEDEICAAGAAIGASFGGSLAATGTS
                     GPGLALKTEAIGLAVMTELPLVIVNVQRGGPSTGLPTKTEQADLLQAMFARNGECPLA
                     VIAAASPADCFNATIEAFTIATRYMTPVILLTDGYIANSAEPWVIPDVKEMHAIEIKH
                     PTEKNDPSGFQPYKRNEDGARPWAIPGTAGLEHRIGGIEKQDLTGNVSYDPANHQAMV
                     NKRAAKIAGIRPAGASFFWTGPEHGDVLLLGWGGTYGSIKSAALDLRKAGVAASACQL
                     RYLNPLPERLGSLLKRFKKVLIPELNSGQLATLIRARYLVDAVTLSKVRGQPFAVNEI
                     VQAANALLAGDVPATIPAAPLPADDVTSGG"
     gene            2903..3919
                     /locus_tag="VLI90_04560"
     CDS             2903..3919
                     /locus_tag="VLI90_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HSV13506.1"
                     /translation="MSTSLPIPTLKPADFASDQDVRWCPGCGDYSILAQTKKVLAKLG
                     VQRENTVFVSGIGCSSRFPYYMNTYGMHSIHGRAPTFATGLKLARPELNVWLVTGDGD
                     GLSIGGNHMLHVIRRNLDVKILLFNNRVYGLTKGQYSPTSVKGMKTKSSPMGTLDAPV
                     YPICFALGCGVTFAARAVDTDVKQLEYVLERAAKHRGTAFVEIYQNCNIFNDGAFDYA
                     TDKDTKLDHVLYLEHGKPLVFGKNRDKGIRLNALEPEVVNITPGQEDGLIVHDEKSRG
                     GSLAYLLSRMQQPDYPEAMGIFRATEHDTYDELVHRQITDAKSQGGEADLQTLLNGSE
                     TWTV"
     gene            complement(4075..>4702)
                     /locus_tag="VLI90_04565"
     CDS             complement(4075..>4702)
                     /locus_tag="VLI90_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSV13507.1"
                     /translation="LALALLLNAKVRGQTIYRTIIFLPSLVPAVASAMVWLWLYNPKL
                     GLINLGLRKIGVGEPPGWLSDPNWSLRALVLMSLWGVGNTVIIYLAGLQDVPRDLYEA
                     AELDGAGVAGKIWHVTIPCISPVIFFNLIIAIIGTIQQFTLPYIMTPNGQPGRATYVF
                     TMYLYDNAFGYLKMGTASGMAWIQLLLILALTGLAFWSSKRWVHYQGK"
BASE COUNT          951 a         1466 c         1457 g          828 t
ORIGIN      
        1 ccaaagagct tgatgacgtt tccgttgagt tggaacgaac tcgcgccact gtcgaactgg
       61 atctggttga actgtgtgaa ggcgccgtag tcgttattgg gtgtgaggcg gtttgagccg
      121 gcaaaatgca cgacgcccgt accaccgccg tcggggttac cgttggtgcc gttccaattg
      181 gcgacatcgc cccagctgct gctggtgccg ctgttgccgt tccaagtggc cggattcgcc
      241 gtcgcgagcg cgacgccact ccgcagcccc acgcccacga tggcgctgat caacatagcg
      301 tgcgctatgc gaggctttgc tgctcgcgcg gcctgagcaa tgtacgtgtt cgaagtctcc
      361 cggcgcatcc cgctgccctc gcaatcccct gattctgccc gtcgatctat gacttaaggt
      421 taatcggttt agcagtatat cggaccgacc cgctttatca acaattttcc gattccctga
      481 gtcgcgaaca cgccgtcgcc taacccaccg catataatgg actaacactc aatccatcgc
      541 gggtggtgga cccgattttt ggtggtgctg gagaaatccg gccgcgcgga agctgcccat
      601 cgctacaatc tttctactca cacttcgcaa acgcgcgcgc gtggcttgcg tttgcgcgat
      661 cgccagcgct gccaagcgcg cccgacgacc gtgacatccc tttgtaaatc gcttgacatc
      721 ggaggcaact cttatggacg gcgtctcttc agttaaggag gaaaccccaa gggagcaagt
      781 tggcctgggc gcgggcccag gcgcagtgcc ccagcaaccg gcgagcggca cggcgtccgt
      841 ggcgccggtc atcaaacaag cgctcgatcg acgaaagtcg atcgtctcgc tcagcaacgt
      901 caccatccgc ttcgccggtg acagcggtga cggcatgcag gtggccggca cgcagttctc
      961 ggatgtgtcg gccctcgttg gtaacgacat cagtacgctg ccggatttcc ccgccgagat
     1021 ccgcgccccc gccggcacgc tcgcgggcgt cagcggttat cagatccact tcgccgattc
     1081 cgacatcttc acgcccggcg atgacgtgga cacgctcatc gcgatgaacc cggcggcgct
     1141 gaagatgaac ctcaggtccg ttcgccccaa cggcaacgtc atcgtcaacg aagatgcgtt
     1201 caccgcggcc gacttgagaa aggccggcta cgaaaccaac ccaatcgaag gcaacacgat
     1261 cgaaggcttc cgcctgatca aggtgccgat tgaacggctc aacgccgagg cgctgaaaga
     1321 ctcggggctg acggcgaagg aaatcgatcg ctgcaagaac tttttcgcgc ttggcctggt
     1381 gtgctggcta tacgaccggc cgctggaacc gacgctgaaa tacatccgcg ccaagttcgg
     1441 caagaagcag gcgaacgttg ccgccgccaa tgaaacggtg ctgaaggcgg ggcacgcgtt
     1501 tggtgagacg gcggaactgt tccccgcgca atatcgcgtg gaaaaagcca agctgccgcc
     1561 ggggcgatat cgcaagatca ccggcaacga agcggtggcg ctgggcatgg tgacggcgtc
     1621 gcggctggcg ggcaaacagc tgttctacgc cagctatccg atcacgccgg ccagcgacat
     1681 cctgcacagc ctcgctgcga tgaaacagtt cggcgtcgtc acgttccagg cggaagatga
     1741 aatctgcgcc gccggcgccg ccatcggcgc cagcttcggc ggatcactcg ccgcgactgg
     1801 aaccagtggg ccgggtctgg cgctgaagac cgaggcgatc ggcctggccg tcatgaccga
     1861 attgccattg gtcatcgtca acgttcaacg cggcgggccg agcaccggct tgccgaccaa
     1921 gaccgaacaa gccgatctgc tgcaggcaat gttcgcccgc aacggcgaat gcccgttggc
     1981 ggtcatcgct gcggcgtcgc cggccgattg tttcaacgcg accatcgaag cgttcacgat
     2041 cgcgacgcgt tacatgacgc cggtcatcct gttgaccgat ggctacatcg ccaatagcgc
     2101 tgagccgtgg gtgattcccg atgtgaagga gatgcacgcg atcgagatca agcatccgac
     2161 ggagaagaac gacccgagcg ggttccagcc gtacaagcgc aacgaagacg gcgcgcggcc
     2221 gtgggcgatc cccggcaccg cgggtctcga gcaccgcatc ggcgggatcg agaagcagga
     2281 cctcaccgga aacgtcagct acgacccggc caatcaccag gcgatggtga acaagcgggc
     2341 ggcgaagatc gccggcatcc gcccggcggg ggcgtcgttc ttctggaccg gccccgagca
     2401 cggtgatgtc ctgctgctgg gttggggcgg cacgtatggt tcgatcaagt cggccgcgct
     2461 ggaccttcgc aaggccggtg tggccgcgtc ggcgtgtcag ctgcggtatc tcaacccgct
     2521 gccggagcgg ctgggttcgc tcttgaagcg attcaagaag gtcctgatcc ccgaactgaa
     2581 cagcgggcag ttggcgacgc tgatccgcgc cagatatctg gtggacgcgg tgacgctgag
     2641 caaagtccgc gggcaaccgt tcgcggtgaa cgagatcgtc caggcggcga atgcgctgct
     2701 ggcgggcgat gtccccgcga cgatccccgc agcgccgctt cccgcggatg acgtgacatc
     2761 gggtggataa gccatcaccg tgacacgggc cgccggcccg tgcgtgaacc tcgggacttt
     2821 cgaaatgcct ggtttttcaa attcataagc agctcgcacg ggccagaggc ccgtgtcacg
     2881 attgagcagc tggagttcga ccatgagcac aagccttccc attccaactt tgaaacccgc
     2941 cgactttgcg tcggaccagg acgtgcgctg gtgccccggt tgcggggatt attcgatcct
     3001 ggcgcagacc aaaaaagtac tcgccaagct cggcgtgcaa cgcgagaaca cggtcttcgt
     3061 ctcgggcatc ggctgctcga gccgattccc gtactacatg aacacctacg gcatgcactc
     3121 gatccacggc cgcgcgccga cgttcgccac gggccttaaa ctcgcgcggc cggagctgaa
     3181 cgtctggctc gtcaccggtg acggcgacgg tctgtccatc ggcggcaatc acatgctgca
     3241 cgtgatccgc cgaaacctcg acgtgaagat cctgctgttc aacaaccgcg tgtatgggct
     3301 gaccaagggc cagtattcgc cgacgagcgt gaagggcatg aagaccaaga gcagcccgat
     3361 gggcacgctg gatgcgccgg tgtatccgat ctgcttcgcg ctaggttgcg gcgtaacatt
     3421 cgctgcccgc gcggtggaca ccgatgtgaa gcagctggag tacgtgctgg agcgcgccgc
     3481 caagcatcgc ggcaccgcgt tcgtcgagat ctaccagaac tgcaacatct tcaatgacgg
     3541 cgcgttcgat tacgcgacgg ataaggacac gaaactggat cacgtgctgt acctcgagca
     3601 cggcaagccg ctggtcttcg gcaagaatcg cgacaagggc attcgcctaa acgcgctcga
     3661 gcccgaagtc gtgaacatca cgcccggcca ggaggacggc ctgatcgtcc acgacgaaaa
     3721 atcccgcggc gggagtctgg cgtacctcct gagccggatg cagcagccgg actatcccga
     3781 ggcgatgggc atctttcgcg cgacggagca cgacacgtac gatgagctgg tccaccggca
     3841 gatcacggac gcgaaatcgc agggtgggga agcggatttg cagacgctgt tgaatggatc
     3901 ggagacgtgg acggtgtgaa ttggaaaatc cgaatgacga aagagcgaat ccccgaatga
     3961 atgacgaatg acgaaatccg aatgtagaaa cgctgcggga ttcttcaccc aggctaaccg
     4021 cgtcagcggt tggcatattc atcgtcattc aagcttcgtc attcggattt ccgatcactt
     4081 cccctggtaa tgcacccacc gtttgctgct ccagaacgcc agccccgtca gcgccaagat
     4141 cagcagcagc tgaatccacg ccatgccgct ggcggtgccc attttcagat acccgaacgc
     4201 gttgtcgtat aggtacatcg tgaagacgta cgtcgcccgg ccgggttggc cgttgggggt
     4261 catgatatac ggcagcgtga actgctggat ggtgccgatg atcgcgatga tcaggttgaa
     4321 gaagatgacc ggcgagatgc aggggatcgt gacgtgccag atcttgccgg ccacgccggc
     4381 gccgtccagc tccgccgctt cgtacaggtc gcgcggcacg tcctgtagcc cagccagata
     4441 aatgatgacc gtgttgccta cgccccagag gctcatcagc accagcgcgc gcagcgacca
     4501 gttcggatcc gacaaccaac ccggcggctc gccgacgccg atcttgcgca ggcccaggtt
     4561 gatgagcccg agcttggggt tgtacagcca aagccaaacc atcgccgacg ccaccgccgg
     4621 caccagcgac ggcaggaaga tgatcgttcg gtagatcgtc tgcccgcgga ccttcgcgtt
     4681 cagcagcagc gccagcgcga gc
//
LOCUS       DATIMH010000269         2547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4100605, whole genome shotgun sequence.
ACCESSION   DATIMH010000269 DATIMH010000000
VERSION     DATIMH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4100605"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..>2547)
                     /gene="galA"
                     /locus_tag="VLI90_04570"
     CDS             complement(57..>2547)
                     /gene="galA"
                     /locus_tag="VLI90_04570"
                     /inference="COORDINATES: protein motif:HMM:NF041462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HSV13508.1"
                     /translation="RRYFKLEEADRARHLELQFDGVGTHCTVWVNGVIAHRNWCGYTS
                     FAIDITPFAHYGEDVNNIAVRVDAEAMEGWWYEGAGIYRHTWLVKRDPVHIATDGVYA
                     NPVRSADGSWSVPVEVTLSSSATAPATVNVETRVIDSAGAEVGHGNAQVTVKPFEASI
                     ARYVIAVASPKLWSVDEPALYHTRTVIKRDDGTIIDEADTQCGFRTIRFDPNEGFFLN
                     DQHLKLQGTCNHQDHAGVGAAMPDSIWDFRVRRLKEMGANAYRCAHNPPAAEFLAACD
                     RMGMLVMDENRNFNTTPEYMGQLQWLIRRDRNHPSVILWSVFNEEPSQGNEQGYEMVR
                     RMSAAVKELDVTRPVTAAQSSAMMNPVNASQAADVAGFNYQHRDYDRYHAANPTKPIT
                     SSEDTSAVMTRGEYTTDHKRAIIDSYDTQFQRWGNTHRNGWREIAERPFLAGGFVWTG
                     FDYRGEPQPLEWPATGSSFGCMDLCGFPKTAYYIHQAQWISDRPIVQIVPHWNWPGRE
                     GKRVHVMVITNAQRIALSLNGKSLGEKPVDKYQMVSWDVPYEAGKLEAIATNDGKEVA
                     RSVVQTGGAPAMLRLIADRASLAGDGRDAQPITVEALDAEGRIVPTANLPVTFDLSGP
                     GAIIGVGNGNPNAHEPEKGNKQSIFNGYAQAIVQSTPGGSGRLTLHAAGDGVAPADVT
                     IDVTPTAAPPEIAPAKPTVIVLAWRMSPMSNERPDPNQSIDRNDVNTWAAVRPGRLTP
                     FNNGKFAVLRATFHPRQTVRESGGQLVMRNVTGKAEVWIDGKLAAEKKDAARGSITAP
                     LPPGEGDRTVSVLIESDPGTPAGLGGVVTVE"
BASE COUNT          383 a          827 c          822 g          515 t
ORIGIN      
        1 aaatcaccct caccctaacc ctctcccacg cgtacgtggg cgaggggacc agaaagttat
       61 tcaaccgtca ccaccccgcc cagaccggcg ggggtgccgg gatctgattc gatcagcaca
      121 ctcaccgtgc gatcgccttc gccgggtggg agcggggcgg tgatgctgcc gcgcgccgcg
      181 tctttctttt cggcggcgag cttgccgtcg atccagacct cggccttgcc ggtgacgttt
      241 ctcatcacca gctgtccgcc ggattctcgc acggtttgac gcgggtggaa cgtcgcgcgc
      301 agcacggcga acttgccgtt gttgaacggc gtcagccggc cggggcgcac ggccgcccag
      361 gtgttgacgt cgttcctgtc gatcgactgg ttcgggtccg ggcgctcgtt cgacatcggc
      421 gacatgcgcc aggctaagac aatcaccgtg ggcttagcgg gcgctatctc cggcggcgcg
      481 gctgttggcg tgacgtcaat cgtgacatcc gccggcgcca cgccgtcgcc agccgcgtgc
      541 agcgtcaagc gcccgctgcc gccgggcgtg gattgcacga tggcctgtgc gtagccgttg
      601 aagatgcttt gcttgttgcc cttctcgggc tcgtgagcgt tcgggttgcc gttgcccacg
      661 ccgatgatcg cgccggggcc gctcaagtcg aacgtcaccg gcagattggc cgtcgggacg
      721 atccggcctt cggcgtcgag cgcctcgacc gtgatcggct gcgcgtcgcg tccgtcacca
      781 gcgaggcttg cgcgatctgc gatcaaccgc aacatcgcgg gcgcgccgcc cgtctggacg
      841 accgatcgcg ccacctcctt gccgtcgttc gtcgcgatcg cttccagctt gcccgcttcg
      901 tacggcacgt cccatgacac catctggtat ttgtccaccg gtttttcgcc aagtgatttg
      961 ccgttgaggc tcagcgcgat gcgctgcgcg ttggttatga ccatcacgtg cacccgtttg
     1021 ccttcgcggc cgggccagtt ccagtgcggg acgatctgca cgatcgggcg atcgctgatc
     1081 cactgcgcct ggtggatgta gtacgccgtc ttgggaaacc cgcacaggtc catgcagccg
     1141 aacgacgacc ccgtcgccgg ccattccagc ggctgcggtt caccacggta gtcgaacccg
     1201 gtccacacga atccgccggc gaggaacggc cgctcggcga tttcccgcca cccgttgcgg
     1261 tgcgtgttgc cccagcgctg gaactgcgtg tcgtacgaat cgatgattgc gcgcttgtgg
     1321 tccgtcgtgt actcgccgcg ggtcatgacg gccgaagtgt cttcggagct ggtgatcggc
     1381 ttagtcggat tggcggcgtg atagcggtcg tagtcgcggt gctggtagtt gaaccccgcc
     1441 acgtcggcgg cttgcgaggc gttcaccggg ttcatcatcg cgctgctctg ggcggccgtc
     1501 accggccggg tcacgtccag ctccttcacc gcggccgaca tccggcggac catctcgtaa
     1561 ccctgctcgt tgccttgcga cggttcttcg ttgaagaccg accacaggat cacgctggga
     1621 tgattgcggt cgcggcggat gagccactgc aactgcccca tgtattccgg cgtcgtgttg
     1681 aagttgcggt tctcatccat caccagcatg cccatccgat cgcacgcggc gagaaactcg
     1741 gcggcggggg gattgtgggc gcagcgatag gcgttagcgc ccatctcttt gagccggcga
     1801 acgcggaaat cccagattga atccggcatg gcggctccga cgccggcgtg gtcctggtga
     1861 ttgcacgtgc cctgcaattt gaggtgctga tcgttgagga agaaaccttc attcggatca
     1921 aagcggatcg tgcggaagcc gcactgggtg tcggcttcgt cgatgatcgt gccatcatcg
     1981 cgcttgatga ccgtgcgcgt gtgatacagc gcgggctcat cgacggacca cagttttggc
     2041 gatgcgacgg cgatcacata tctcgcgatc gacgcttcga acggtttgac tgtgacttgc
     2101 gcgttgccgt ggccgacttc ggcgccggcg gaatcgatga ctctcgtctc gacattgacc
     2161 gtcgccggcg cggtggcgct gctgctcagc gtcacttcga ccggcacgct ccacgagcca
     2221 tctgcgctgc gaacgggatt ggcgtaaacg ccatcggtgg cgatgtgcac cgggtcgcgc
     2281 ttcaccagcc aggtgtggcg atagatgccg gcgccttcgt accaccagcc ttccatcgcc
     2341 tcggcatcga cgcgcacggc gatgttgttg acgtcctcgc cgtagtgcgc gaacggcgtg
     2401 atgtcgatcg cgaacgaagt gtacccgcac caatttcggt gcgcgataac gccgttgacc
     2461 cagacggtgc agtgcgtgcc aacgccatcg aactgcagct ccaggtgccg cgcgcggtcg
     2521 gcttcctcga gcttgaaata tcgccgg
//
LOCUS       DATIMH010000270         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4682118, whole genome shotgun sequence.
ACCESSION   DATIMH010000270 DATIMH010000000
VERSION     DATIMH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4682118"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VLI90_04575"
     CDS             <1..668
                     /locus_tag="VLI90_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HSV13509.1"
                     /translation="VELDVIHRVVGIGTDWLANLSPGDEVDVLGPLGNAFTLPGQAQT
                     AVMVGGGVGIPPMLYLATKLVGRNAVAIAGALTRDLLPLMITGEPAGAEPRPIIDEFA
                     RHQIPTVITTDDGSFGYRGFVTQALDEYLDTHRTGPTPVLYTCGPEPMMKRIADIAKR
                     RGLQCQIAVERAMACGMGTCQSCCIRLKKPDPNQPPLVGKDWAYRLACTDGPVFRARD
                     LLW"
     gene            777..1064
                     /locus_tag="VLI90_04580"
     CDS             777..1064
                     /locus_tag="VLI90_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HSV13510.1"
                     /translation="MTTIGMHYDVIPGKEEEFERGFIGVLEVLKTLPGHVESHMYEDV
                     QSLGSYVILSQWESREAFDRFMHSPEFAKAVAWGKAEILRSRPRHKIYTNA"
     gene            1113..>2267
                     /locus_tag="VLI90_04585"
     CDS             1113..>2267
                     /locus_tag="VLI90_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13511.1"
                     /translation="MKSDRPARFRGQRLFPGALLLLAITLIAYIPIFRAGFVWDDDSY
                     VEQNATLRTLDGLYEIWTNWHATPQYYPLVHTSFWVEYHLWGATSALGYHIDNVLLHA
                     LSAILIWRLLNRLNVPGALLAAAIFAVHPVHVESVGWITERKNVLSLVFYLLALRSYL
                     RSTGSEPVLRDYAFSLLFFLLALLSKTVTATLPAAILLIFWWKRGRIRVRAVTPLLPF
                     FVLAAVCGSITGYLERTQVGARGPEWAALTPLHRVLIASRAVWFYAGKLLVPWRLTFI
                     YPRWSIDPRDPLQWLYLVALIAVIAGLWLRRRRLGRGPLVAVLFFIGTLTPALGFVNV
                     YPMRYSFVADHFQYHASIGLIALAAACIARFKPAGLIIVALLIALTFHRCL"
BASE COUNT          374 a          821 c          645 g          427 t
ORIGIN      
        1 gggtggaact ggacgtgatc caccgcgtgg tcggcatcgg caccgattgg ctggccaact
       61 tgtcgcccgg cgacgaagtc gacgtcctcg ggccgctggg caacgccttc acacttccgg
      121 gccaagcgca gaccgccgtc atggtcggcg ggggcgtcgg catcccgccg atgctgtacc
      181 tcgcgacgaa gctcgtcggc cggaacgccg tcgccatcgc cggcgcgctg acgcgcgacc
      241 tgctgccgct gatgatcacc ggtgaacccg ccggcgctga accgcgtccg atcatcgacg
      301 aattcgcccg gcaccagatc cccaccgtca tcaccaccga cgacggcagc ttcggttacc
      361 gcgggttcgt cacgcaggct ttggacgagt acctcgacac ccaccgcacc ggcccgacgc
      421 cggtgctcta cacgtgcgga cccgagccga tgatgaagcg catcgccgac atcgccaagc
      481 gccgcgggct gcaatgccag atcgccgtcg agcgcgcgat ggcctgcggc atgggcacct
      541 gccagagctg ctgcatccgc ctgaaaaagc ccgaccccaa ccaacccccg ctggtcggca
      601 aagactgggc atatcgcctc gcttgcaccg acggcccggt atttcgcgcc cgggacttgt
      661 tgtggtaacg gaattggaat cagttgctcg ttgctagttg ctagtgaata cggggggatt
      721 gcggtactct tactagcaac tagcaacgaa caaccagcaa ctgattccaa acccccatga
      781 ccaccatcgg catgcattac gacgtgatcc ccggcaagga agaggaattc gagcgcggct
      841 ttatcggtgt gctcgaggtc ctcaagacat tgcccgggca cgtcgagagc cacatgtacg
      901 aagacgtgca gtcgctcggc agctacgtca tcctcagcca atgggaatcg cgcgaggcgt
      961 tcgatcgctt catgcacagc cccgaattcg ccaaggcggt cgcctggggc aaagccgaaa
     1021 tcctgcgcag ccgaccccga cacaagatct acaccaacgc ctaagcgcgt tgctgacgcc
     1081 caaggcgcgt gcgaaaccgc aaaccgtccc acgtgaaatc cgaccggcct gcccgcttcc
     1141 gcggacagcg tctgttcccc ggcgcgctcc tgctcctggc gatcaccctc atcgcctaca
     1201 tccccatttt ccgcgccggc ttcgtctggg acgacgattc ctatgtcgag caaaacgcaa
     1261 cgctccgcac gctcgatggg ttgtacgaga tctggaccaa ctggcacgcg acgccgcagt
     1321 attacccgct cgttcacacc agcttctggg tcgaatacca cctctggggc gcgaccagcg
     1381 cgctcggcta tcacatcgac aacgtcctgc tccacgcgct cagcgcgatc ctgatctggc
     1441 ggctgctgaa ccgcctgaac gtccccggcg cgttactcgc cgccgcgatc ttcgccgtgc
     1501 atcccgtgca cgtcgaatcc gtcggatgga ttaccgaacg caagaacgtt ttgtcgctcg
     1561 tgttttattt gttggccttg agatcgtacc tccgtagcac gggctcggag cccgtgctac
     1621 gtgattacgc tttctccctt ctattcttcc tcctcgcgct cctcagtaaa accgtcaccg
     1681 ccaccctgcc cgcagcgatc ctgctcatct tctggtggaa acgcggtcgc atccgcgtgc
     1741 gcgccgtcac gccgctgctg ccgttcttcg tcctggccgc cgtgtgtggc tcgatcaccg
     1801 gctatctcga gcgcacgcaa gtcggcgccc gcggccccga atgggccgct ctcacgcccc
     1861 tgcaccgcgt gctgatcgcc tcgcgcgccg tctggttcta cgccggcaaa ctgctcgtgc
     1921 cctggcggct gacgttcatc tacccgcgct ggtcgatcga tccgcgcgac ccgctgcagt
     1981 ggctctacct cgtcgcgctc atcgccgtca tcgcaggtct gtggctccgg cgccgccggc
     2041 tcggccgcgg gccgctggtc gcggtgctgt tcttcatcgg cacgctgacg ccggcgctgg
     2101 gcttcgtgaa cgtttacccg atgcgctatt cgttcgtcgc cgaccatttc cagtatcacg
     2161 cgagcatcgg cctgatcgcc ctcgccgccg cgtgcatcgc gcgcttcaaa ccggccgggt
     2221 tgatcatcgt cgcgctgctg atcgccctca cgttccaccg ctgcctc
//
LOCUS       DATIMH010000271         3954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3813456, whole genome shotgun sequence.
ACCESSION   DATIMH010000271 DATIMH010000000
VERSION     DATIMH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3813456"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1395
                     /locus_tag="VLI90_04590"
     CDS             136..1395
                     /locus_tag="VLI90_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HSV13512.1"
                     /translation="MSDAPIQISPAALAAADDDRFHRFRLIEWWDQAKLRNARVLVVG
                     AGALGNEIVKNLALLGVGNVLIADLDRVENSNLSRSVLYRKQDNGRFKADVAAASARD
                     IYPDIVAHAFNGNIVYDLGLGVFHWADVIMGGLDNREARLSINRNCYRCSKPWIDGAI
                     QQIDGTARVFIPETHALGRANTACYECTMSQTDWRLLQLRRSCNLLSKTEMETGKTPT
                     TPTISSIIAGVQCQEAIKILHGMDTIAGKGWVFNGITADSYLTEFQRKDACYSHDPLE
                     EIVAMDWSAHTTTARDALSEARRRLGAGAELELARDVLEKLVCPKCGATEELFASLGN
                     VKAEKANCPNCPGVRREVVTFYKIRGDESFLDRPLAAIGVPAFDILIARTPERATGLE
                     LSADAGNVLGPLPRTRAVANEDELEWT"
     gene            1392..2207
                     /locus_tag="VLI90_04595"
     CDS             1392..2207
                     /locus_tag="VLI90_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HSV13513.1"
                     /translation="MTDAGTIPSERDISAIAAGDWPVRELVGHVTGRREARFQVVFRQ
                     SVLDQIHLHGQSTTEVEVCGVLVGNGYRDARGPYLLIEHCIRGTAAASRSTNVTFTAD
                     TWQHIQATMDREHAKAKIVGWYHTHPGFGIFLSDMDVFICENFFNLPWQVAFVYDPIS
                     GEEGDFLWRAGRPERDPVLIEHDVTPASASIALISKQDAMAAPSIADDRLVELQRRVR
                     QLERRQRVMLLAIAFLVAFATFWLMQNAPTPPAVKGNGSQPTTMPRAGLGQKR"
     gene            2303..3577
                     /locus_tag="VLI90_04600"
     CDS             2303..3577
                     /locus_tag="VLI90_04600"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSV13514.1"
                     /translation="MPGEKIQIQWDDLKSRKVDQRLREQEALERNRRQADGQLLSDGF
                     ESTAAPSSSLWYNAILTMAIFGLLGGVLAWCAGEALRLRPDPKAEARAHMREIGYFTQ
                     RVAEGKMTAAEADSTINVLRAPYGSNPYFAIYSDTSLSDAEKDAQLRALDQREAWKAF
                     IAKLLSFGLSGMLIALCLSIAEPVVDHNVQGAVVNGAVGAAAGLMGGLIVSLFVDRLY
                     AALRGAGSGGGGDETGYQQMLARAITWGVLGLFLTLAPGLVMRNAKKFFIGMAGGLLG
                     GLVGGALFDPVAAATDGNQHVSRLVALLAIGVVAGAATGLIENAAKSGWLKVTVGLIA
                     GKQFILYRNPTFIGSGPECQIYLFRDPKVGRRHAAVHIVPGGFELENLPMGGTTLIND
                     KPVERARLRCGDRIQIGATSLAFQEKEQTNAE"
     gene            3746..>3954
                     /locus_tag="VLI90_04605"
     CDS             3746..>3954
                     /locus_tag="VLI90_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSV13515.1"
                     /translation="MATLIYNHAGVRLGGVIGGRVLIGRRLGRGVSIDDPAVSRLHAW
                     IDHRVGTYVLSDAGSRTGTFVNGGG"
BASE COUNT          730 a         1244 c         1302 g          678 t
ORIGIN      
        1 tgggctttgg tcattggaac ttcattggca tttggagctt gagccttggt cattagccat
       61 tcgaaatcac cctcacccca gcccgcaagg ctgtgcctcc tcccacaagt acgtgggaga
      121 gggggcgaaa cctgaatgtc cgacgctccg attcaaatct cccccgcggc gctcgccgcc
      181 gcggacgacg accgcttcca ccgctttcga ctcatcgaat ggtgggacca ggccaagctg
      241 cgcaacgccc gcgtgctggt cgtcggcgcc ggcgcgctcg gcaacgagat cgtcaagaac
      301 ctggcgctgc tcggcgtcgg caatgtcctc atcgccgacc tcgaccgcgt cgagaactcc
      361 aacctctcgc ggtccgtcct ctatcgcaag caagacaacg gccggttcaa agccgacgtt
      421 gccgcggcgt cggcgcggga catttatccc gacatcgtcg cccacgcgtt caatggcaac
      481 atcgtctacg acttaggcct gggcgtcttt cattgggccg acgtcatcat gggcggactc
      541 gataaccgcg aggctcgcct cagcatcaac cgcaattgtt accgctgcag caagccatgg
      601 atcgacgggg cgattcagca gatcgacggc accgcccggg tgttcatccc ggagacgcac
      661 gcgctgggcc gggcgaacac cgcctgttac gagtgcacga tgagccagac cgactggcgg
      721 ctgctgcagc tgcggcggtc gtgcaacctg ctgagcaaga ccgagatgga aaccggcaag
      781 acgccgacca cgccgacgat cagctcgatc atcgccggcg tgcaatgcca ggaggcgatc
      841 aagatcctgc acggcatgga cacgatcgcc ggcaaaggct gggtcttcaa cggcatcacc
      901 gccgattcgt acctgacgga gtttcaacgc aaagacgcct gctacagcca tgatccgctg
      961 gaagaaatcg tcgcgatgga ttggtcggcg cacacgacga cggcgcggga tgcgctgagc
     1021 gaagcgcgcc ggcggcttgg agcaggcgct gagctggaac tggcccgcga cgtgctcgag
     1081 aagctggtct gtcccaagtg cggcgcgact gaagaactct tcgcctccct cggcaacgtg
     1141 aaggccgaaa aggccaactg ccccaactgc cccggcgttc gccgggaggt tgtcacgttc
     1201 tacaaaatcc gcggcgatga atcgttctta gatcgcccgc tcgcggcgat cggtgtgccg
     1261 gcgttcgaca tcctcatcgc gcgcacgccg gaacgcgcga ccggactgga actttccgcc
     1321 gatgcgggga acgtcctggg accgttgccg cggaccaggg ccgtcgcgaa cgaagacgaa
     1381 ctggagtgga cgtgaccgac gccggaacga ttccaagcga gcgcgacatc agcgcgatcg
     1441 ccgccggcga ttggccggtg cgcgagttgg tcggtcacgt caccggcagg cgcgaagcgc
     1501 gcttccaggt cgtcttccgc caatcggtcc ttgaccagat ccatctgcac ggccaaagca
     1561 ccaccgaagt ggaagtgtgc ggcgtcctcg tcggcaacgg ttaccgcgac gcgcgcgggc
     1621 catacctgct gatcgagcat tgcatccgcg gcacggccgc ggcgtcgcga tcgaccaacg
     1681 tcacgttcac cgccgacacc tggcagcaca tccaggcgac gatggaccgc gagcacgcga
     1741 aggcgaaaat cgtcggctgg tatcacacgc atccgggctt cggcatcttt ctgtcggaca
     1801 tggatgtgtt catctgcgag aacttcttca acctgccctg gcaggtggcg ttcgtgtacg
     1861 acccgatcag cggcgaggaa ggcgattttc tctggcgcgc cgggcgaccg gagagagacc
     1921 cggtcctgat cgagcacgac gtgacgccgg cgtcggcgag catcgcgctg atcagcaagc
     1981 aggacgcgat ggccgcgcca tcgatagcgg atgaccggct ggtcgagctg cagcgtcgcg
     2041 tgcgccaact agaacggcgg cagcgcgtga tgctgctagc gatcgcgttc ctcgtcgcgt
     2101 tcgcgacgtt ctggttgatg caaaacgcgc cgacgccgcc ggcggtgaag ggaaatgggt
     2161 cgcagcccac cacgatgccg cgcgcgggct tgggacagaa gcgataaaag aaggctgcgt
     2221 ctggtcccct ctgccacaag acctgcggtc ggcgtacgtg ggcgagggga ccagggtgta
     2281 aactgcattg gagccgtacg taatgcccgg tgagaagatc caaattcagt gggacgatct
     2341 caagtcgcga aaggtcgatc agcggctgcg cgagcaggag gcgctggagc gcaaccgccg
     2401 gcaggcggat gggcagttgc tgagcgacgg cttcgagtcg acggcagcgc cgtcgtcgag
     2461 cctctggtac aacgcgattc tcacgatggc gatcttcggc ctgctgggcg gcgtgctggc
     2521 gtggtgcgcc ggcgaagcgc tgcggctgcg gccggatccc aaggccgagg cccgcgccca
     2581 catgcgcgag atcggctatt tcacccagcg cgtcgccgag ggcaagatga ccgccgccga
     2641 ggccgacagc acgatcaacg tgctgcgcgc gccgtacggc agtaatccat attttgcgat
     2701 ctatagcgac acctcgctaa gcgacgcgga gaaagatgcg cagctgcgcg cgctcgatca
     2761 gcgcgaggcg tggaaggcgt tcatcgccaa actcctgagc ttcggcctga gcggcatgct
     2821 gatcgcgctg tgcctgtcga tcgccgaacc cgtcgtcgat cacaacgtgc aaggcgcggt
     2881 cgtcaacggc gcggtcggcg ccgccgccgg attgatgggc ggcttgatcg tgtcgctctt
     2941 cgtcgatcgg ctttacgccg cgctccgcgg cgccggcagc ggcgggggtg gtgacgaaac
     3001 gggatatcag cagatgctcg cccgcgcgat cacctggggc gtgctcggcc tgttcctcac
     3061 gctcgcgccg gggctggtca tgcgcaacgc gaaaaagttt ttcatcggca tggccggtgg
     3121 actgctcggc gggctggtcg gtggcgcgct gttcgatccc gtcgcggcgg cgaccgatgg
     3181 caatcagcat gtgagccggc tcgtcgcgct gctggcgatc ggcgtcgtcg cgggggcggc
     3241 cacggggttg atcgagaacg ccgccaagag cggttggttg aaagtgacgg tcggcctgat
     3301 cgccggcaag cagttcatcc tgtatcgcaa ccccacgttc attggcagcg gccccgagtg
     3361 ccagatctat ctgttccgcg accccaaggt cggccggcgt cacgccgccg tgcacatcgt
     3421 ccccggcggg ttcgagctgg aaaacctgcc gatgggcggg acgacgctca tcaacgacaa
     3481 gccggtggaa cgcgctcggc tgcgctgcgg cgatcgaatc caaatcggcg cgacgagctt
     3541 ggcgtttcag gagaaggagc agacgaatgc agaatgatga atgcagaatg cagaatggac
     3601 gacggggagc ttttaaccac agatttcaca gattacacag attgatgtgg ggactttctg
     3661 aatctgtgta atctgtgaaa tctgtggtta aaactctcaa tttgattctg cattctgcat
     3721 tcatcattct gcatttcccc accccatggc caccttgatc tacaaccacg ccggcgtccg
     3781 cctcgggggc gtgattgggg gccgcgtgct gatcgggcgg cgcctgggcc gcggcgtttc
     3841 gatcgatgac ccggcggtgt cgcgcctgca cgcgtggatc gatcaccgcg tcggcacgta
     3901 tgtgctgagc gacgccggca gccggaccgg caccttcgtt aacggcggcg gcat
//
LOCUS       DATIMH010000272         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1217435, whole genome shotgun sequence.
ACCESSION   DATIMH010000272 DATIMH010000000
VERSION     DATIMH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217435"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VLI90_04610"
     CDS             complement(<1..551)
                     /locus_tag="VLI90_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13516.1"
                     /translation="MTKPHEFPGKLIIVEGIDGSGKSTQLQLAMRYLQAKGLPVFFTE
                     WNSAELVKTVTKRGKKKMTLTPLTFSLLHATDFAHRLVHNILPPLKAGMIVLADRYAY
                     TAFARDVVRGCDPTWVRRVYSFAPRPDLALYFNVPIDVSSARILSGRAQLKDYEAGMD
                     LHLAPDKVSSFKIFQSRILTEYE"
     gene            complement(707..2296)
                     /locus_tag="VLI90_04615"
     CDS             complement(707..2296)
                     /locus_tag="VLI90_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HSV13517.1"
                     /translation="MPIHKTTRPAQRRAVAAAPAHPVEFRIAAIDVGSNSLHMIIAQV
                     DPDGAITTLWRMKEMVGLGRISFPARRLSGEAMERAIATLRRFHEAAHRRQCEKILTV
                     ATSAVREAQNGGDFLERVRRELGMNIRVVSAREEARLIYLGVRHGTPLKSAPTFMIDI
                     GGGSVEFIVADKTKPMMLESRKIGAARMTARYVKSDPIAGPDLKSLLGNYDRELTPLC
                     EQILALKPIEALATSGTCENLAAMCTALYGSPEGVIEREPLNKLVSKLLESRSKDRAE
                     YKGLDDQRKDQIIAGALLVNELFRRLNLERMTVCKSALREGILVDYLSRHLPEVSIRR
                     EVPDPRRRSVLDLARRCDYHQTHAEQVARLCMQLFDQLKPMHELNKNGRELIEYAALL
                     HDIGWHISRDQHHKHGMYLILHGNLKGFSPEEVQIMANIVRYHRKKGPTLEHEAFASL
                     SGKAKKIVRAGAALLRISDGLDRSHSTVVSSIRCTLHRRRVDVTIKARGDAELEMWGG
                     QRKMDLFAQTFGRSLSFREVE"
BASE COUNT          436 a          789 c          725 g          485 t
ORIGIN      
        1 ttttcatact ccgtcaggat gcggctctga aaaatcttga agctcgacac cttatccggc
       61 gccaggtgca gatccattcc ggcctcgtaa tccttcaatt gcgcccggcc cgaaagaatg
      121 cgcgcagagc tcacatcgat cggcacatta aaatagagcg ccaggtccgg ccgcggcgca
      181 aaactgtaaa cccgccgcac ccacgtcgga tcgcaaccgc gcaccacgtc gcgcgcgaag
      241 gccgtatacg cataacgatc cgccagcacg atcatccccg ctttcagcgg cggcaggatg
      301 ttgtggacca atcgatgggc aaaatccgtc gcgtgcagca gcgaaaacgt cagcggcgtc
      361 agcgtcatct tcttcttgcc gcgcttggtc accgtcttca ccagctcagc gctgttccat
      421 tcggtgaaga acaccggcag gcccttggcc tgcaggtagc gcatcgccag ctgcagctgc
      481 gtgcttttgc cggaaccgtc gatgccttcg acgatgatca gcttgccggg aaattcgtgg
      541 ggtttggtca atgcatccat acaaccgcgt ctttccccct ctcccacgta cgcgtgggag
      601 agggccgggg tgagggtgat ttggaaaatc aaacggctat cgacttctca aatcaccctc
      661 accctaaccc tctcccacgc gtacgtgcga gaggggacta gaaaaatcac tccacttccc
      721 ggaagctgag gcttcggccg aacgtctgcg cgaacaaatc catctttcgc tgcccgcccc
      781 acatttccag ttcggcgtcg ccgcgcgctt tgatcgtcac atcgactcgc ctgcggtgca
      841 gcgtgcagcg gatcgatgaa accacggtgc tgtggctgcg atctaaaccg tcggagatgc
      901 gcagcaatgc tgcgccggcg cgcacgattt tcttggcctt gcccgacaac gacgcgaagg
      961 cctcatgctc cagcgtcggg cccttcttgc ggtgatagcg cacgatgttc gccatgatct
     1021 gcacttcctc cggggaaaaa cctttgaggt tgccgtgcag gatcaggtac atgccgtgct
     1081 tgtggtgctg gtcgcgcgag atgtgccagc cgatgtcgtg cagcagggcg gcgtattcga
     1141 tcagctcgcg cccattcttg ttcagctcgt gcatgggctt gagctgatcg aacagctgca
     1201 tgcacaatcg cgcgacctgc tcggcgtgcg tctggtgata atcgcatcgt cgcgccagat
     1261 cgagcacgct gcgccgccgc gggtctggca cttcgcggcg gatcgacacc tccggcaggt
     1321 gccgcgacag gtaatcgacc aggatgcctt cgcgcagcgc ggatttgcaa accgtcatcc
     1381 gctccaggtt caaccggcgg aacaattcgt tcaccagcag tgcgcccgcg atgatctgat
     1441 ccttgcgctg atcatccaac cctttgtatt cggcgcgatc cttgctgcgg ctttcgagca
     1501 gcttgctgac cagcttgttc aatggctcgc gctcaatcac tccctcgggt gagccgtaca
     1561 gcgcggtgca catcgcggcg aggttttcgc acgtgccgct ggtggcgagc gcttcgatgg
     1621 gcttcaacgc caggatctgc tcacacagcg gtgtcaattc gcggtcgtag ttacccagca
     1681 gcgacttcaa atccggcccg gcgatgggat cgctcttcac atatcgcgcg gtcatgcggg
     1741 ccgccccgat cttgcggctc tcgagcatca tcggcttggt tttgtcggcg acgatgaact
     1801 cgacgctccc gccgccgatg tcgatcatga acgtgggcgc gctcttcagc ggcgtgccgt
     1861 gccgaacgcc caggtagatc agccgcgcct cttcgcgcgc gctgacgacg cggatgttca
     1921 tcccaagctc gcggcggacg cgctcgagga agtccccgcc gttctgcgct tcgcgcacgg
     1981 ctgacgtcgc gacggtaagg atcttttcgc actgccgccg gtgggccgct tcgtggaaac
     2041 gccgtagcgt ggcgatcgcg cgctccatcg cttcaccgct caatcgccgc gcggggaaac
     2101 tgatccgtcc aaggccgacc atctccttca tccgccacag cgtggtgatc gccccgtcgg
     2161 gatcgacctg tgcgatgatc atgtgcaggc tgttggaccc gacgtcgatg gcggcgatgc
     2221 ggaactcgac cggatgagcc ggcgcggcgg caaccgcgcg ccgttgcgcg gggcgggttg
     2281 ttttatggat cggcataggc gttggcaccc gcgtcaagtc tagcgttctt atcgaccgtg
     2341 tcgaaggggt gcgtaagggg cgataactac caggacagat gcgcgaaacg cacgcccaca
     2401 cgcaccatca ccgccgcggg cgacagccag cgggc
//
LOCUS       DATIMH010000273         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3814401, whole genome shotgun sequence.
ACCESSION   DATIMH010000273 DATIMH010000000
VERSION     DATIMH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3814401"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..334
                     /locus_tag="VLI90_04620"
     CDS             26..334
                     /locus_tag="VLI90_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HSV13518.1"
                     /translation="MAKGCTHIAGIQNVTPSALGCEECLKSGSRWLHLRICRTCGHVG
                     CCDDSPNKHATAHFHATGHPVIEGYDPPEGWGWCYVDEVVFDLSKRKTPHNGKIPRYY
                     "
     gene            516..1016
                     /locus_tag="VLI90_04625"
     CDS             516..1016
                     /locus_tag="VLI90_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13519.1"
                     /translation="MILGMSLSTFVLVHTIISIVAIIAGFIVMFGMLKSTRPGGLTAL
                     FLVLTILTSATGFLIPPLVSDKLLPSHIIGALSLVLLAIACIALYAMKLSGWWRWIYA
                     VTALLALYFNTFVLVIQSFLKIPALTAVAPGNPPSGPVFAVVQGVVLVFFVLMIIGAV
                     RRFWPK"
     gene            1041..1865
                     /locus_tag="VLI90_04630"
     CDS             1041..1865
                     /locus_tag="VLI90_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSV13520.1"
                     /translation="MATITTKDGVEIYYKDWGKGQPIVFSHGWPLSADDWDAQMLFFL
                     NNGFRVIAHDRRGHGRSSQTGDGHDMDHYADDLAALTAHLDLKNAVHVGHSTGGGEVV
                     HYIARHGESRVAKAAILSAVPPLMVQTAANPGGLPKSVFDGFQTALAANRSEFYRAIA
                     AGPFYGYNRPGANASEPIIQNWWRQGMMGGAKAHYDGIVAFSQTDFTEDLKKINVPVL
                     VMHGDDDQVVPYADSGPLSAKLLKNGTLKTYKGFPHGMPTTEAAVINADLLAFIRS"
BASE COUNT          370 a          631 c          629 g          416 t
ORIGIN      
        1 cggcgcttta ggatagaggt cgagcatggc aaaaggctgc acccacatcg ccggcattca
       61 aaacgtcacg ccgagcgcgc tcggctgcga ggagtgtctc aagagcggca gccgctggct
      121 gcatttgcgg atctgccgca cctgcggcca cgtcggctgc tgcgacgatt ccccgaacaa
      181 gcacgcgacc gcacattttc acgccaccgg ccatcccgtg atcgaaggct acgatccgcc
      241 ggaaggctgg ggctggtgct atgtcgacga agtcgtgttc gacctgtcga agcgaaaaac
      301 gccacacaac ggcaagatcc cgcgctacta ttgattcgaa tctcccctgc ctcgatttgt
      361 gacaaccttg caaaaatttg atgcagcttg cggctttcct cgatacgcgt gcgctgacgc
      421 gcatgcgtgg gttgtcgcaa gacgtgactg acgcgtctgg caatttggtt ccctgcgtcg
      481 taacctcgga taggttctaa aagcgggggc gcatcatgat actcgggatg agtctgtcta
      541 cgttcgttct ggtccacacc atcatcagta tcgtcgcgat cattgccggc ttcatcgtga
      601 tgtttggcat gcttaagtcg acgcggcccg gcggcctcac cgcgctgttc ctggtgctga
      661 cgatcctgac cagcgcgacc gggtttctga ttccgccgct ggtctccgac aagctgctgc
      721 cgtcgcacat catcggcgcg ctgtcgctgg tgctcctcgc catcgcctgc atcgcgctct
      781 atgcgatgaa gctgtcgggt tggtggcgct ggatctatgc ggtcaccgcg ctgctggcgc
      841 tgtacttcaa caccttcgtg ctggtgattc agagtttctt gaagattccg gcgttgaccg
      901 cggtagcgcc cggcaacccg ccatccggac cggtattcgc tgtcgtgcag ggcgtggtgc
      961 tggtgttctt cgtgctgatg atcatcggcg ccgtcagacg cttctggccg aaatgaccgc
     1021 gtagttagaa ggagtcccct atggccacca tcaccaccaa ggacggcgtc gagatctatt
     1081 acaaggactg gggcaagggt cagcccattg tgttctcgca cggctggccg ctgtcggcgg
     1141 acgactggga cgcgcagatg ctgttcttcc tcaataacgg ttttcgcgtc atcgcccacg
     1201 accgccgcgg tcatggccgt tccagtcaga ccggcgatgg ccatgacatg gaccattacg
     1261 ccgacgatct ggcggcgttg acggcgcatc tcgatctcaa gaacgccgtt cacgtcgggc
     1321 attccaccgg cggcggcgag gtggtgcatt acatcgcccg gcacggcgag agccgggtgg
     1381 cgaaggccgc gatcctctcc gcggtgccgc cgctgatggt gcagacggcg gccaatcccg
     1441 gcggcctgcc gaagtcggtg ttcgacggat ttcagacagc gctcgccgcc aaccgttcgg
     1501 agttctaccg cgcgatcgcg gcggggccgt tctacggcta caaccgtccc ggcgccaacg
     1561 cgtcggaacc catcatccag aactggtggc ggcaaggcat gatgggcggg gcgaaagccc
     1621 attacgacgg catcgtcgcg ttctcgcaga ccgacttcac cgaggatctg aagaagatca
     1681 atgtccctgt cctggtgatg catggcgacg acgaccaagt cgtgccttat gcggactccg
     1741 gcccgttgtc ggcaaaactt ctgaagaacg gcacgctgaa gacctacaag gggtttccgc
     1801 acggcatgcc gaccacggaa gcggctgtta tcaacgccga cctgctggcg ttcatcagat
     1861 catgaaagtg gtcctgttcg gcgccaccgg catggtgggc caaggcgtgc tgcgcgaatg
     1921 cctggtcgat gccggcgtcg agcgcgtgct ggtgatcggg cgcagcccga ccggatcgca
     1981 taacgccaag ctgcgtgaag tccagcacga ggatttcacg agtttttccg caatcgaatc
     2041 cgaact
//
LOCUS       DATIMH010000274         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4971384, whole genome shotgun sequence.
ACCESSION   DATIMH010000274 DATIMH010000000
VERSION     DATIMH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4971384"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..700
                     /locus_tag="VLI90_04635"
     CDS             122..700
                     /locus_tag="VLI90_04635"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HSV13521.1"
                     /translation="MTNDNRSRCPWAKSELMIHYHDTEWGVPLHDDQKLFEFLILEGA
                     QAGLSWETILRKRDNYRAAFDNFDPNVVAKYGQRKIRQLLADAGIVRNRLKITSAIDN
                     AKAFLVVQKEFGSFDKYIWQFVDGVPRVNRIKTIKDVPVTSVESDTMSKDLRRRGFRF
                     VGSTICYAHMQATGMVNDHLLTCWRRAAGTDA"
     gene            complement(915..>1645)
                     /locus_tag="VLI90_04640"
     CDS             complement(915..>1645)
                     /locus_tag="VLI90_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HSV13522.1"
                     /translation="CVNCPRAVALSAVYYCFACLLDELVPLNGGCFGNIDVLTTPGTV
                     VDARYPAAVVAGNTETSQRIVDVVFGALAKAMPDRIPAASCGTMNSVALGGEGWTYYE
                     TIGGGSGAGPGYAGAPAVQCHMTNTLNTPAEAIELQYPLRVRRFERDPGSGGAGEFPG
                     GDGIVREIEVLAKVDGTILSDRRVTRPYGLNGGNAASVGENWAVVPGVRDFLLAGKAQ
                     FTLEPGERLRILTPGGGAWGGAAR"
BASE COUNT          312 a          530 c          515 g          288 t
ORIGIN      
        1 cgaaaccgca agcgactgct gcgccgatcg cgatgatttt cgttcactca cttctcagca
       61 agatccttcg acggcgcacg agtacgtgct ccgctcagga tgacaattag gacataatgc
      121 gatgaccaac gacaaccgat cccgctgccc gtgggccaag agcgaattga tgatccatta
      181 tcacgacacc gaatggggcg ttccgcttca cgatgaccag aagctgttcg agtttctgat
      241 cctcgaaggc gcgcaagcgg ggctgagttg ggagacgatc ctgcgcaagc gcgacaacta
      301 tcgcgcggcg ttcgacaact tcgacccgaa cgtcgtcgcg aaatacggcc agcggaagat
      361 ccgccagctg ctggccgacg cgggaatcgt gcgcaatcgc ctgaagatca cgtcagccat
      421 cgacaacgcc aaggcgtttc tcgtggtgca gaaagagttc ggctcgttcg acaaatacat
      481 ctggcagttc gtcgacggcg tgccgcgggt caatcgcatc aagacgatca aagacgtgcc
      541 ggtgacgtcg gtggagtcgg acacgatgag caaggacctc cgccgccgcg gatttcgctt
      601 cgtcggttcg acgatctgct acgcccacat gcaggcgacg gggatggtga acgaccattt
      661 gctgacgtgc tggcggcgcg ccgctgggac tgacgcttga tcctccgtct taagcgccaa
      721 ggccgccaag acgccaagag cgccaagaag agaaaaaatc ttgagccaca gatgaacgcg
      781 gatgcacgcg gaccagtttc gacagctcga ttttcatgcg cgtgcatctg cgtctatccg
      841 tggcacattc tccggcgttt gctcttggcg ctcttggtgt gcgcgataga cccgggccga
      901 tagccattga tctatcatcg cgctgcgcca ccccacgccc cgccgccggg ggtgaggatt
      961 cttaggcgct cgccgggttc gagggtgaat tgcgctttgc cggccagcag gaaatcgcgc
     1021 acgccgggga cgacggccca gttctcgccg acgctggcgg cgttgccgcc gttcaggcca
     1081 tacggccggg tgacgcggcg atcgctgagg atcgtgccgt ccactttggc cagcacttcg
     1141 atctcgcgga cgatgccgtc gccgccgggg aattcaccgg cgccaccgct gccggggtcg
     1201 cgctcgaatc ggcggacgcg cagcggatat tgcagctcga tcgcctcggc gggcgtgttc
     1261 agcgtgttgg tcatgtgaca ctgcaccgcc ggcgcgccgg cgtaacctgg ccccgccccg
     1321 ctgccgccgc cgatcgtttc gtaatacgtc cagccttcgc cgccgagcgc gacgctgttc
     1381 atcgtcccgc agctggcggc gggaatgcga tcgggcatcg ccttggccag cgcgccaaac
     1441 acgacatcaa cgatgcgctg gctcgtctcg gtgttccccg cgaccaccgc cgcgggatac
     1501 cgcgcatcga ccacggtgcc gggcgtggtc agcacgtcga tgttgccgaa gcagccgccg
     1561 ttcaacggca ccagctcatc gagcagacaa gcgaagcagt agtagacagc ggacagcgcg
     1621 acggcgcgcg ggcagttcac acacc
//
LOCUS       DATIMH010000275         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2659849, whole genome shotgun sequence.
ACCESSION   DATIMH010000275 DATIMH010000000
VERSION     DATIMH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2659849"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VLI90_04645"
     CDS             <1..1172
                     /locus_tag="VLI90_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HSV13523.1"
                     /translation="EFYDRLVDALLEAGITPYLTLFHWDYPYELYCRGGWLNAESPIW
                     FEQYAQVLAKRLGDRVRHWITLNEPQCFLALGHREGVQAPGLRLAWMDVLRALHHSLL
                     AHGRAVSALREHCALKPEIGWAPVGEVKFPLSNDSAEVDAARARTMSAGERNLWNNTL
                     FSDPACLGHYPEDALRTWGKDFPKFTQGDLRVIHQTIDFYGLNIYRGQPVRRGEDGRA
                     IDVEPAPGCATNAYQWRVEPASLYWGPRFLNERYKLPIHITENGFAGLDWVAMDGAVH
                     DPQRVDFTRRYLRELSRAIDEGIDVRGYFHWSLLDNFEWADGYRQRFGLVHVDYQTQR
                     RTPKDSAYWYSSLIKNNGQEIAGDASPYVGDADGRARQPRVSVSVRKPRSITIST"
     gene            complement(1342..2682)
                     /locus_tag="VLI90_04650"
     CDS             complement(1342..2682)
                     /locus_tag="VLI90_04650"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSV13524.1"
                     /translation="MSLNVLVIDDEASIRKALSACLQADAHRPVAVGSAAEAMDAIRR
                     RSFDLALIDLNLGKDAGLDLITSLLAESPWTKVVVITAYASVDTAVESMRRGAHDYLP
                     KPFTPAQVSALTTKIEQLRQLEQRVAGLQEALGEAGPEADLTSSSSPGMQRALQLAHQ
                     VASSDATILIRGDSGTGKGVLAKAIHAWSARANQRFSVVSCPALSPQLLESELFGHVR
                     GSFTGAARDNPGRIAASEGGTLFLDEIGDLPLPLQPKLLRFVQDREYERVGDHTPRKA
                     DVRVIAATNVNLEDAVKAGTFRQDLLYRLNVIQIVIPPLRERPDDIAPMAETLLAYFA
                     KQNRKPALRLSDDARTALRHYAWPGNVRELRNVVERAAILCSGDEVIPQQLLLETATA
                     APAPVRIGDPVPMEKIEELHICSVLASAKSIEEAAKILNMDTVTLWRRRKKYGV"
BASE COUNT          489 a          911 c         1051 g          591 t
ORIGIN      
        1 tcgaatttta cgatcggctc gtggacgcgc tgctcgaagc cggcatcacg ccgtacctca
       61 cgctgttcca ttgggattat ccgtacgaac tttattgccg cggcgggtgg ttgaacgcgg
      121 agtcgccgat ctggttcgag caatacgcgc aggtgctggc gaagcgcctg ggcgaccgcg
      181 tgcggcattg gattacgttg aacgagccgc aatgtttcct ggcgctgggc caccgcgaag
      241 gggtgcaggc gccgggcctg cggctggcgt ggatggacgt gctgcgggcg ctgcaccatt
      301 cgctgctggc ccatggccgg gcagtcagcg cgctgcgcga gcactgcgcg ttgaagccgg
      361 aaatcggctg ggcgccggtc ggcgaggtga agttcccgct gtcgaatgat tcggccgaag
      421 tggacgccgc ccgggcgcgg acgatgtcgg ccggcgagcg aaacctgtgg aacaacacgc
      481 tgttcagcga tccggcttgt cttgggcatt atcccgaaga cgcgctgcgc acctggggca
      541 aggactttcc gaagttcacg cagggcgatt tgcgcgttat ccaccagaca atcgatttct
      601 acgggctcaa tatctaccgc gggcaaccgg tgcggcgcgg tgaagatgga cgggccatcg
      661 acgtcgagcc ggcgccgggt tgcgcgacca acgcgtacca atggcgcgtt gagccggcga
      721 gcttgtattg gggaccgcgc tttctgaacg agcgctacaa gctgccgatc cacatcaccg
      781 agaacggctt cgccgggctc gattgggtag cgatggacgg cgcggtgcac gacccgcagc
      841 gggtcgattt cacgcgccga tatctgcgcg agctgagccg ggcgatcgac gaaggcattg
      901 atgtgcgcgg ctacttccac tggtcgctgc tggacaactt cgagtgggcc gacggctatc
      961 gccagcgatt tgggctggtg cacgtggatt atcaaacgca gcggcgaacg ccgaaggatt
     1021 cggcgtattg gtattcgagc ttgatcaaga acaatggtca ggagatcgcg ggggatgcca
     1081 gtccctacgt aggcgacgcc gacggccgcg cgcggcagcc gcgcgtctcg gtgtcggtgc
     1141 gcaagccgcg gtcgatcacg atctcgacgt gatgatctgc tgagaaccga ggcaacagac
     1201 cacgccaggt tcggagcgta ccggcgacga accggattct ttttgctcga tgcgcggcag
     1261 aatccgggtc gttggagtac ctccgaaccc ggcgtgggaa agtgctgcgc gcgtctgctc
     1321 attgggggga gaaagtcgta atcacactcc gtatttttta cgcctgcgcc atagcgtgac
     1381 cgtgtccata ttcaggatct ttgccgcttc ctcgatcgac ttggcgctgg cgaggacgct
     1441 gcagatgtgc agttcttcga tcttctccat cggcaccggg tcgccgatgc gcaccggggc
     1501 cggggcggcg gtggcggttt ccaacagcaa ttgttgcggg atcacttcgt cgccggagca
     1561 taggatggcg gcgcgctcga cgacattgcg cagctcgcgc acgttgccgg gccaggcgta
     1621 gtgccgcagg gcagtgcggg catcatccga caggcgcagc gccggcttgc ggttttgttt
     1681 cgcgaaatac gccagcagcg tctccgccat cggggcgata tcgtccggcc gctcccgcag
     1741 cggggggatg acgatctgga tcacgttcaa gcgatacagc aagtcctggc ggaatgttcc
     1801 tgctttaact gcatcttcta aattcacatt ggtcgctgcg atgactcgga catccgcctt
     1861 gcgcggtgtg tggtcgccga cgcgctcgta ctcgcggtcc tgcacgaagc gcagcagctt
     1921 cggttgcagc ggcagtggca ggtcgccgat ctcgtcgagg aacagcgtgc cgccttcgct
     1981 ggcggcgatg cggccggggt tatcgcgggc ggcgccagtg aacgagccgc gcacgtgacc
     2041 gaataattca ctctccaata actgtggtga aagagcgggg caggagacga cgctgaagcg
     2101 ctggttggct cgcgcgctcc aggcgtggat cgccttggcg agcacgcctt tgccggtgcc
     2161 gctgtcgccg cggatgagga tcgtcgcgtc gctgctcgcg acttgatgcg cgagttgcaa
     2221 cgcccgctgc atgccgggac tgctgctgct ggtcagatcg gcttcgggcc cggcttcgcc
     2281 gagcgcttcc tgcaggccgg ccacgcgctg ctcgagctgg cgcagttgct cgatcttcgt
     2341 cgtcagcgcg ctgacctgcg cgggtgtgaa cggcttgggc aggtaatcgt gcgcgccgcg
     2401 ccgcatcgat tcgacggcgg tgtccaccga cgcgtacgcc gtgatcacga cgactttcgt
     2461 ccagggactt tccgccagca aagacgtgat gagatcgagc cccgcgtcct tgcccaagtt
     2521 caggtcgatc agcgccagat cgaacgaccg ccggcggatg gcgtccatcg cctcggcggc
     2581 cgatccgacc gctactggcc ggtgcgcgtc ggcctgcaag caggcggaca acgccttgcg
     2641 gatgctggct tcgtcgtcga tgaccaggac gttgagggac attggcgggt attgtcgcgc
     2701 cggggcggat ggaatcaacg cgcgtttgtt ggtcgcatcc acgccgggtt cggaggtact
     2761 ccgacgaagc gagattctgc cgcgcgcgag aagcatcggg gtcgtcggag cacgtccgaa
     2821 cccggcgtgt ggggcattgc gatatacttg atcttgggtt cgcggctcac acatgctttt
     2881 cacgtcgcct atctggttac tcggtcttct gccatgggcg ggcgtggtgg tgtacctgct
     2941 gcgcgggcgt ccgcagcgaa gggctgttcc atttcttgaa ctctggtcgc ccgtgtcggt
     3001 cgcgccgaag ccgcggcgcg cggttcggcg gccaccgctg tt
//
LOCUS       DATIMH010000276         7606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1505752, whole genome shotgun sequence.
ACCESSION   DATIMH010000276 DATIMH010000000
VERSION     DATIMH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505752"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1997
                     /locus_tag="VLI90_04655"
     CDS             <1..1997
                     /locus_tag="VLI90_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSV13525.1"
                     /translation="GAVETLLILGINPAYTAACDVPFADALRNLSNDKRGGDYAAFTA
                     HLATHVDETSFACQWHLPRAHWLESWGDVRAFDGTTSIIQPLIAPMFGGRSDWELMEA
                     ALARPDPTGMEIIRDHWIATVGAGGFDSWWMKSLQAGVIENTASKRRDPPPLRDDVLA
                     TLNAGSSDGTEIIFRTDPNVWTGEFTNNAWLQELPKPFTKLTWDNAVIISPGLAQRLG
                     SGGDGGMLRDGSVVRVEYRGQTLEAPIVLLPGQPDEVITLHLGYGRSRGGQVLMDEGK
                     PRGFKAYSLRTSDAPWHSRGSVTIESTGGAAFLAITRGHHAMAIQPGMPGVKTSLKPE
                     VIGAAGDSDDDLELRNRRIVRTATLDQFKTDPHVVEKLEPLSKKPLLSLYPGWDYEHG
                     LQWGMSIDMTACIGCNACVIACQAENNIPVVGKGEVGRQREMHWIRIDDYFGGSLENP
                     SVFHQPVPCMHCENAPCEYVCPVGATTHSDEGLNEMTYNRCVGTRYCSNNCPYKVRRF
                     NFLLYSDYQTPELKMLHNPDVTVRSRGVMEKCTYCIQRIDRTRIEMEREVLDLQERAK
                     KATTEEERIALLQQADQRGRQIINGLQTACQQSCPTVAIVFGDIRDPQADVSKLKKEP
                     TDYSILAELTTKPRTTYLARISNPNPAMQAAEIAAGGASL"
     gene            2003..3433
                     /gene="nrfD"
                     /locus_tag="VLI90_04660"
     CDS             2003..3433
                     /gene="nrfD"
                     /locus_tag="VLI90_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HSV13526.1"
                     /translation="MTTPPPPLAASNGTYAPGKNAPVLHQGQTFESVTEKISGLVLDR
                     PVHRSYLLALGLAGTLTMILGYTVFYLIWKGVGIWGVNVPVAWGFAIANFVWWIGIGH
                     AGTFISAMLLLMRQDWRTSINRFAEAMTLFAVACAGMFPLMHLGRPWMFYYLFPYPNT
                     MKLWPQFRSPLVWDVFAVGTYFTVSLIFWYIGLVPDLATMRDRAKGKLAWAVYGILAL
                     GWRGASRHWKRYQTAYLLLGALAAPLVISVHSVVGMDFAAALVPGWHSTIFPPYFVAG
                     AIFSGFAMVLTLAIPLRAIYGLHGLVTIRHIDNCCKLMLVNGLIVAYGYGSELFMGWY
                     GGAKYDQYMDLNRMFGPYAWSWWTIMFCNVLTPQALWFRQVRQNAPTVFLVAIIVQIG
                     MWTERFVIVITSLHRDFVPSAWGYYEPTKWDWSTLIGTMGFFLLCFFLFVRLLPMISM
                     AEVRELVHSTGGGRHEPVSFSPKYAE"
     gene            3441..4025
                     /locus_tag="VLI90_04665"
     CDS             3441..4025
                     /locus_tag="VLI90_04665"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HSV13527.1"
                     /translation="MASASQPSTPQEPTHADEKPELYGLAAIFDSPEAILEAAGKVHA
                     AGYRRAEGYTPFAVEGLSDALGFKHTGVPLIVLVGGAFGAIGGYFMLWYANVISYPWN
                     VGGKPPNSWPAFIPITFEMTVLGASLLALFGMLLLNGLPSPYHPMFHAPRFDRASQTE
                     FFLCIESADAQFDPARTRAFLEGLNPLAIVEVPW"
     gene            4022..4726
                     /locus_tag="VLI90_04670"
     CDS             4022..4726
                     /locus_tag="VLI90_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSV13528.1"
                     /translation="MSAGTQHIQPGEPQPPPGLSGRARNVDTGALFLLFLVFCLVGGA
                     ITFLIVLPSWRPTANMHRQPYFKPYRPSEMFADGMSERPLVSGVVPRPADRSPGIPYV
                     AVREPGPANFPVLAQTDRIPMPITRPLLERGQEQYTIYCSMCHGDTGRGDGMIVHRGF
                     YPPPSYHNQRLRDLPDSHFFNVITNGYGAMFSYANRVTPEDRWAITAYIRALQLAVQR
                     SPELSQQLQKPRGVQP"
     gene            4723..5931
                     /locus_tag="VLI90_04675"
     CDS             4723..5931
                     /locus_tag="VLI90_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13529.1"
                     /translation="MSESPVIVFPAAGAQRFRAWAIIVAAAWIVCVIIGWIFEPDVFY
                     RSYLFAWLFWLGVTLGAIGVVMLHHLMGGHWGTPTRRLGEHAGMTLPLMIVLFIPVAI
                     GLHSLFIWSRADELAKDPTLRHKAAYLNVPFFLLRACFYFASWLLLAWYLRSRSLRFE
                     RTRNPATERAMHNVSAGGMLYYFISMSFAGVDWIMSREPHWFSTVFGLLVVAAQGISG
                     LCFIILVLSILYRIGILRGVVDEDQFNDLGSLLLTFVIVWAYLAFVQLLVIWMGNKQD
                     EIPWYVQRLSNGWWWIGLLLVLLHFLVPFIVLLMRQLKRSARAMLWLCAGLLIMRAVD
                     VFWMVGPSGDDPQPLLRHILSWLDFVLPIGMGGAWLLMFLWLADGSPLLVSMPTGAPA
                     AERADGLQGA"
     gene            5954..6391
                     /locus_tag="VLI90_04680"
     CDS             5954..6391
                     /locus_tag="VLI90_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13530.1"
                     /translation="MAQAQDQLPSADALQRGYESITVSNRGLLIFLICFILTAVVLHW
                     AMWVLIKYYAGMTRDADMPRSDVTRVDPFVDPRLQPSEPHPYLPWQDLASWRQEQQQV
                     FDQLGWRQNQDNGRPHIPDDVVAALAARYATTGPSKTRGGGQP"
     gene            6388..7263
                     /locus_tag="VLI90_04685"
     CDS             6388..7263
                     /locus_tag="VLI90_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HSV13531.1"
                     /translation="MSRRGVPSSVALAMLLGLLQLLQCAPARAGITMAALPKTAFIEQ
                     HTGAVIPPELIFTDESGNDVQLGQMLAAKPTLLVLAYFSCPDLCPMTLRHLTQGLNGI
                     ALRAGDEFQVVVISFDSRDTPAAAAQQRLNYTRGYRHGDDADGWHFLTGRQPMIDRLT
                     AAVGYHCALDADVNRFAHAAGVMVVSPGGRLSHYFFGVDYSPADLEAAVRDAAAGRAT
                     VVDQPDQQYCYAYNPATGRRGRQIVAALRCSGVCWMLALFGYIGTKLTQEFRSRRSTP
                     VSLPPGEGRREGVLR"
     gene            7260..>7606
                     /locus_tag="VLI90_04690"
     CDS             7260..>7606
                     /locus_tag="VLI90_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13532.1"
                     /translation="MSSTQDIPFAPIQASTAAASVDHLFLFELSVGMFFVALIFTLIV
                     YFALRYHRRSDDEVPPRVGTHYGLEAAWSIIPFAFMMVYFFWGAKIYVNLKKPVTHAM
                     EVHVTGKQWMWKI"
BASE COUNT         1288 a         2367 c         2489 g         1462 t
ORIGIN      
        1 ccggcgctgt ggagacgctg ctgatcctcg gcatcaatcc cgcgtacacc gccgcgtgcg
       61 atgtgccgtt tgccgatgcg cttcgcaacc tgtccaacga caagcgcggc ggcgattacg
      121 cggcattcac cgcccacctg gcgacgcacg tggacgagac ctccttcgcc tgccaatggc
      181 atctgccgcg ggcgcactgg ctggaatcgt ggggcgacgt ccgggcgttc gacggcacga
      241 cgtcgatcat ccagccgctg atcgcgccga tgttcggcgg tcgctcggat tgggaactga
      301 tggaggccgc gctcgcccgg ccggacccaa ccgggatgga aatcatccgc gatcattgga
      361 tcgcaaccgt cggcgcaggc ggcttcgatt cgtggtggat gaagagcctg caggcggggg
      421 tgatcgagaa cacggcgagc aaacgccgcg accccccgcc gctgcgcgat gatgtgctgg
      481 cgacattgaa cgcgggaagc agcgacggca ccgaaatcat cttccgcaca gacccgaacg
      541 tctggaccgg cgagttcacc aacaacgctt ggctccagga actgccaaag cccttcacga
      601 agctgacgtg ggacaacgcg gtgatcatca gcccgggcct agcgcagcgc ttgggcagtg
      661 gtggcgacgg cggaatgctg cgcgacggca gcgtcgtccg cgtggaatac cgtggccaaa
      721 cgctcgaggc gcccatcgtg ctgctgccgg ggcagccgga tgaagtgatc acgttgcacc
      781 tcggttatgg ccgaagccgc ggcgggcagg tgctgatgga cgaggggaaa ccgcgcggat
      841 tcaaggctta ttctctgcgc accagcgacg ccccctggca cagccgcggc agcgtaacaa
      901 tcgaatcgac gggcggcgcg gcgtttctcg ccatcacgcg cgggcatcac gcgatggcca
      961 ttcaacccgg catgccgggt gtgaagacgt cgcttaagcc ggaggtgatc ggcgccgccg
     1021 gcgattccga tgacgacctg gagctgcgca atcgccgaat tgtccgcacc gccacgctcg
     1081 atcagttcaa gacggacccg catgtcgtcg agaagctgga accgttgtcg aagaagccgc
     1141 tgttgtcgct gtatcccggt tgggactacg agcacggcct gcaatggggt atgtcgatcg
     1201 acatgaccgc gtgcatcggc tgcaacgcgt gcgtgatcgc ctgccaggcg gagaacaaca
     1261 tccccgtcgt cggcaaaggc gaagtcggcc ggcagcgcga gatgcactgg atccgcatcg
     1321 acgattactt cggtggctcg ctggagaacc ccagcgtgtt ccaccagccg gtgccgtgca
     1381 tgcactgcga gaatgcgcct tgcgaatatg tctgtcccgt cggcgcgacg acgcacagcg
     1441 acgaggggct caacgaaatg acctacaacc gctgcgtcgg cacgcggtat tgctcgaaca
     1501 actgcccgta taaagtccgc cggttcaact tcctgttgta cagcgattac cagacgccgg
     1561 agctgaagat gctgcacaac ccggacgtca ccgtgcgcag ccgcggcgtg atggagaaat
     1621 gcacctactg cattcagcgc atcgatcgca cgcggatcga gatggagcgc gaggtactcg
     1681 atcttcagga gcgcgcgaag aaggcgacga cggaggaaga gcgcatcgcg ctgttgcagc
     1741 aggcggacca gcggggccgc cagatcatca acggcttgca gaccgcctgc cagcagagtt
     1801 gcccgacggt ggcgatcgtc ttcggagata ttcgcgatcc gcaagccgac gtgtcgaagc
     1861 tgaaaaaaga gccgaccgac tacagcatct tggcggaact gacgacgaag ccgcgcacaa
     1921 cctatctggc gcggatcagc aaccccaatc cggcgatgca ggctgcggaa atcgcggcgg
     1981 gaggcgcatc gctatgacga cgatgaccac ccccccgccg ccgctcgccg cttcgaacgg
     2041 cacctacgcg ccaggtaaga acgcgccggt gttgcatcag ggacagacgt ttgaatcggt
     2101 gacggaaaag atcagcgggc tggtgctcga ccggccggtg catcgctcgt acctgctggc
     2161 gctcggactg gcgggcacgc tgacgatgat cctcggctac accgtctttt acctcatctg
     2221 gaaaggcgtc ggcatctggg gcgtgaacgt cccggtggca tggggatttg cgatcgcaaa
     2281 cttcgtctgg tggatcggga tcggtcacgc cggcacgttc atcagcgcga tgctgctgct
     2341 gatgcggcag gattggcgga cgagcatcaa tcggttcgcc gaagcgatga cgctcttcgc
     2401 cgtcgcgtgc gccggcatgt ttccgctgat gcacctgggc cggccgtgga tgttttacta
     2461 tctgttcccc tacccgaaca caatgaagct ttggccgcag ttccgcagcc cgctggtgtg
     2521 ggacgtgttc gcggtgggaa cctatttcac ggtctcgctg atcttctggt acatcggact
     2581 cgtcccggat ctggcgacga tgcgcgaccg cgccaaaggc aagcttgcct gggcggtgta
     2641 tggcattttg gcactgggtt ggcgcggggc gtcgcggcac tggaagcgat atcagaccgc
     2701 ctacctgctg ctgggcgcgc tggcggcgcc gctggtgatc tccgtgcaca gcgtcgtcgg
     2761 catggacttc gccgccgcac tcgtgccggg ttggcacagc acgatcttcc cgccctactt
     2821 cgtcgccggc gcgatcttta gcggcttcgc catggtattg acgctggcca tcccgctgcg
     2881 cgcgatctat gggcttcacg gtctggtcac cattcgccac atcgacaatt gctgcaagct
     2941 gatgctcgtt aacggcctca tcgtcgccta cggctacggg tccgaactgt tcatgggttg
     3001 gtacggcggc gccaaatacg atcagtacat ggacctgaac cgcatgttcg gtccgtacgc
     3061 gtggtcgtgg tggacgatca tgttctgcaa cgtcctgacg ccgcaggcgc tgtggttccg
     3121 gcaagtccgg cagaacgcgc cgacggtgtt tctcgtcgcc attatcgtgc agatcggcat
     3181 gtggaccgag cgcttcgtca tcgtgatcac cagcctgcac cgcgacttcg tgcccagcgc
     3241 ctggggatat tacgagccga ccaagtggga ctggagcacg ctgatcggca cgatgggttt
     3301 cttccttctt tgctttttcc tgttcgttcg attgctcccg atgatctcga tggcagaagt
     3361 ccgcgagctg gtgcactcca ccggaggcgg gcggcacgag ccggtgagct tttcaccgaa
     3421 gtacgcagag tgaacgcgct atggccagcg ccagccaacc atccacaccg caggaaccga
     3481 ctcacgcgga cgagaagccg gaactctatg gactggcggc gatcttcgat tcgcccgagg
     3541 cgatcctgga agccgcgggc aaagttcacg cggcgggata tcgccgggcc gaaggatata
     3601 cgccgttcgc ggtggaagga ctgagcgacg cgctcgggtt caagcacacg ggtgtgccgc
     3661 tgatcgtgct ggtcggcggg gcgtttggcg cgatcggcgg ttacttcatg ctctggtacg
     3721 ccaacgtcat cagctacccg tggaacgtcg gcggcaaacc gcccaacagc tggccggcgt
     3781 tcattcccat tacgtttgag atgacggtgc tgggcgcgtc gttgctggca ttgttcggca
     3841 tgttgctgct gaacgggctg ccgtcgccgt atcacccgat gttccacgcg ccgcgattcg
     3901 accgggcgag ccagacggaa ttcttcctgt gcattgagtc ggccgatgcg cagttcgacc
     3961 cggcgcggac gcgggcgttt ttagaagggc tcaatcccct ggcgatcgtg gaggtgccct
     4021 ggtgagcgct ggcacgcagc acattcaacc gggcgaaccc cagccaccgc cgggactgag
     4081 cgggcgggcg cgcaatgtgg acaccggcgc gctgttcctg ctgtttctgg tcttctgcct
     4141 ggtgggcggc gccatcacgt ttttaatcgt gctgccgagt tggcggccga cggcgaacat
     4201 gcatcgccag ccgtacttta aaccgtatcg gcccagcgaa atgttcgccg acggcatgtc
     4261 cgagcgcccg ctggtcagcg gcgtggtgcc gcgcccggcc gaccgatcgc ccggcattcc
     4321 atacgttgcc gtgcgcgaac cgggaccggc gaacttcccc gtgctggcgc agaccgaccg
     4381 cattcccatg ccgatcacac ggccactgct ggagcgcggg caggagcagt acacgatcta
     4441 ctgctcgatg tgccacggcg acaccggtcg gggtgacggc atgatcgtcc atcgcggctt
     4501 ctacccgcca ccgtcgtatc acaaccagcg attgcgcgat ctgccggact cgcacttctt
     4561 caacgtgatc accaatggtt acggcgcgat gttcagctat gccaaccgcg tgacgcccga
     4621 agatcgctgg gcgatcaccg cttacattcg cgcgctccag ctggcggtgc agcgatcacc
     4681 ggagctgtcg caacaactgc agaaaccgcg gggggtgcag ccatgagcga atccccggtc
     4741 atcgtattcc ccgccgctgg ggcacagcgg ttccgcgcct gggcgatcat cgtcgccgcg
     4801 gcgtggatcg tctgcgtcat cattggatgg atcttcgagc ccgatgtctt ctatcgctcg
     4861 tacctgttcg cctggctgtt ctggctcggc gtgacgctgg gcgcgatcgg cgtcgtcatg
     4921 ctgcaccacc tgatgggcgg gcattggggc acgccgacac gccgcctcgg cgagcacgcg
     4981 ggcatgacct tgccgctgat gatcgtcctg ttcatcccgg tcgccatcgg cttgcattcg
     5041 ctgtttatct ggtcgcgcgc ggacgagctg gcgaaggacc cgacgctgcg ccacaaggcg
     5101 gcgtacctga acgtgccgtt cttcctgctg cgggcgtgtt tctacttcgc cagctggttg
     5161 ctgctggcgt ggtatctgcg cagccggtcg ctgcggttcg aacgcacgcg caacccagcg
     5221 accgagcgcg cgatgcacaa cgtcagcgcc ggtgggatgc tgtactactt catctcgatg
     5281 agcttcgccg gcgtggactg gatcatgtcg cgcgagccac actggttctc caccgtcttc
     5341 ggtctgctgg tcgtcgcggc ccaggggatc tccgggctgt gcttcatcat cctggtcctg
     5401 tcgatcctct atcgcatcgg tatcttgcgc ggcgtggtcg atgaagatca gttcaacgac
     5461 ctcggcagcc tgctgctgac gttcgtgatc gtctgggctt acctggcgtt cgtgcagctg
     5521 ctggtgatct ggatggggaa caagcaggac gagattccgt ggtacgtgca gcggttgtcc
     5581 aacggctggt ggtggatcgg cctgctgctg gtgctgctgc atttcctcgt gccgttcatc
     5641 gtgctgttga tgcgacaact caagcgcagc gcgcgggcga tgctctggct gtgcgccgga
     5701 ctgctcatca tgcgggcggt ggatgttttc tggatggtcg gacccagcgg tgacgacccg
     5761 cagccgctgc tgcggcacat cctcagctgg ttggatttcg tgctgccgat cggcatgggc
     5821 ggcgcctggc tgctgatgtt cctttggctt gcggatggtt cgccgctgct cgtcagtatg
     5881 ccaaccggcg cacccgccgc ggaacgcgcc gatggcttgc aaggcgcata aggagaacgc
     5941 gcgcgatcgc tttatggcac aggcacagga tcaacttccg tcggccgacg cgctgcagcg
     6001 cgggtacgag tcgatcaccg tcagcaaccg cgggctgctg atctttctta tctgcttcat
     6061 cttgacggcg gtggtactgc attgggcgat gtgggtgctg atcaagtact acgccgggat
     6121 gacgcgcgat gcggacatgc cgaggtcgga tgtcacgcgc gtcgatccgt ttgtcgatcc
     6181 gcgcctgcag cccagtgagc cgcacccgta tttgccgtgg caggacctgg cgtcgtggcg
     6241 gcaagagcag caacaggtct ttgatcagct cgggtggcgt cagaatcagg acaacggaag
     6301 gccgcacatt ccagatgacg tggtggcggc gctggcggcg cgatatgcga cgacggggcc
     6361 gagcaagacg agaggaggtg gccagccgtg agccggcgag gcgttccatc ttctgtcgcc
     6421 ttggcgatgc tgctgggttt gctccaactg ctgcaatgcg cgcctgcccg ggcgggaatc
     6481 accatggctg cgttgccgaa gacggcgttc atcgagcagc acaccggcgc ggtgattcca
     6541 ccggaactga tcttcaccga tgaaagcggc aacgacgtac aactgggcca gatgctggcg
     6601 gccaagccga cgctgctggt gctggcctat ttctcgtgtc ccgacctttg cccgatgacc
     6661 ctgcggcatc tcacgcaggg gttgaacggc atcgcgctgc gcgccgggga tgagtttcag
     6721 gtcgtggtca tcagcttcga ttcgcgcgac acaccggcgg ccgcggcaca gcagcggttg
     6781 aactacacgc gcggctaccg ccacggtgac gacgccgacg gctggcattt cctgaccgga
     6841 cgccagccga tgatcgaccg gttgaccgcc gcggttgggt atcactgcgc gctggacgcg
     6901 gatgtgaatc gcttcgcgca cgcggccggg gtgatggtgg tttcgccggg ggggcgcttg
     6961 tcgcactatt tcttcggcgt cgattattca ccggcggacc tggaagccgc cgtgcgcgac
     7021 gccgccgccg gtcgagcgac ggtcgtggat cagccggacc agcaatactg ctatgcgtac
     7081 aaccccgcga ctggccggcg cggccggcag atcgtcgcgg cccttcgatg cagcggcgtc
     7141 tgctggatgc tggcgctgtt cggatatatc ggcacgaagc tgacgcagga gttccgctcg
     7201 cgccgctcga caccagtttc gctgcctccc ggtgagggcc gtagagaggg ggtgctgcgg
     7261 tgagcagcac gcaagacatc cccttcgccc cgattcaagc ctccaccgcc gcggcgtcgg
     7321 tcgatcatct gtttttgttc gagctgtcgg tgggcatgtt cttcgtcgcc ctgatcttca
     7381 cgctcatcgt ctatttcgcg ctgcgctacc accgccgatc ggatgacgaa gtgccgccgc
     7441 gcgtgggcac gcattacggg ctggaagccg cgtggtcgat catccccttc gcgttcatga
     7501 tggtctattt cttctggggc gccaaaatct atgtcaacct caagaaaccg gtgacgcacg
     7561 cgatggaagt gcatgtgacg ggcaagcagt ggatgtggaa gatcga
//
LOCUS       DATIMH010000277         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1505784, whole genome shotgun sequence.
ACCESSION   DATIMH010000277 DATIMH010000000
VERSION     DATIMH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505784"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VLI90_04695"
     CDS             <1..702
                     /locus_tag="VLI90_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HSV13533.1"
                     /translation="PDIDLTHYAERHAAVKDIMGATALPIMVDCDDGYGDVKNVTYTV
                     RGYEALGVQGIFIEDQQAPKQCGHMDNKKVVPAEVMVGKIKAAVAAKRDDFFILARSD
                     ALAAEGMDNTLRRGEKYLKAGADGIYIEGPESVEQLKMIGKTFGSEPLATSILENGGK
                     TPWVSPKEMGKMGFSMVLYPTTLLFRATYALQQAAADLREGTALNKDEAVDMKAFEKL
                     VDLDHWRAIEKKFGS"
     gene            746..1906
                     /locus_tag="VLI90_04700"
     CDS             746..1906
                     /locus_tag="VLI90_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSV13534.1"
                     /translation="MAVQAGSGPGWIPDLLEHHALHELADALRARTEAISVEWEKLVE
                     RTIPSADQLTFEQIRDDQPKILAMIADALEAASADEARGLLGASLEHGEVRFHQGYVE
                     GDLLIEYRLMRRVVIEQVEDQLQRRLTTIEDLALDMGLDIACHRTMLAFTNQQRTQIM
                     AAAESERLFLSFLSHDVRNSLGSVTLMLKVLRDRLSRTKQFGDEVIELDTLSQLISST
                     MSGMERLLQGESLRRGAVAVARQPIDLRRLVADVTRQQQRRAEEKGITILVEVPEEMI
                     AHSDPELITLVLQNLLDNAVKFTDRGQVRIRIERRGDTFHLLVIDNGPGVPPELLKVL
                     SQPFHRGETGGRPGMGLGLAIATRAAQMLGAELRIDPPGKEGGATFRLILPA"
     gene            2106..>2675
                     /locus_tag="VLI90_04705"
     CDS             2106..>2675
                     /locus_tag="VLI90_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV13535.1"
                     /translation="MTLNEPPDGGDISQTNSNATRAAVAAAVLPHLDAAFNLARWLTR
                     DEHDAADVVQQAYLRAMTAADTFRGGGGAKSWLLTIVRNTAFDRLRRDKTRRQEELGD
                     DLPMTPSDAEACDPQAILLRAANAERVRAAIEELPDGIREVIVLREMEELSYKEVAAV
                     VGVPIGTIMSRLARGRQRLQQLLSEPGEST"
BASE COUNT          531 a          760 c          948 g          436 t
ORIGIN      
        1 ccggacatcg atctcacgca ctacgccgag cgccacgcgg ccgtcaaaga catcatgggc
       61 gcaacggcgc tgccgatcat ggtcgattgc gacgacggtt acggtgacgt gaagaacgtc
      121 acgtacaccg tgcgcggcta cgaagcgctg ggcgtgcagg gcatcttcat cgaagaccag
      181 caggcgccca agcaatgcgg gcacatggac aacaagaagg tggttccggc cgaggtgatg
      241 gtcggcaaga tcaaggccgc cgttgcggcc aagcgtgatg atttcttcat cctggcgcgc
      301 agcgatgcgc tggccgcgga aggcatggac aacacgctgc gccgcggcga gaagtatctg
      361 aaggcgggcg cggacggcat ctatatcgag ggtccagagt cggtcgagca gctgaagatg
      421 atcggcaaaa cgttcggcag cgaaccgctg gcgacgagca tattggagaa cggcggcaag
      481 acgccgtggg tttcgccgaa ggagatgggg aagatggggt tcagcatggt gctgtacccc
      541 acgacgctgc tgttccgcgc gacgtacgcc cttcagcagg cggcggcgga cctgcgcgag
      601 ggaacggcgc tgaacaagga cgaggcggtg gacatgaagg cgttcgagaa gctggtggat
      661 ctcgaccact ggcgggcgat cgagaagaag ttcggctcat gaggctcaaa agtcgcgcac
      721 attaatgcgc ggaaggagcg ttcgcgtggc ggtgcaggca gggtcagggc cggggtggat
      781 tccagacctg ctggagcacc acgcgctgca cgaactagcc gacgcgctgc gggcgcgaac
      841 cgaagccatc agtgttgaat gggaaaagct ggtcgaacga acgattccgt cagccgatca
      901 attgaccttc gagcagatcc gcgacgacca gccgaaaatc ctggcgatga ttgccgacgc
      961 gctcgaggcg gccagcgcgg acgaagcgcg cggattgctc ggggcttcgt tggaacatgg
     1021 ggaagtccgc tttcatcaag gctacgtcga aggcgatctg ctgatcgaat accgtctgat
     1081 gcgccgggtc gtgatcgaac aggtggaaga tcaacttcag cgtcgcctga cgactatcga
     1141 agacctggcg ctggacatgg gcctggacat cgcctgccat cgcaccatgc tggcgttcac
     1201 caatcagcag cgcacgcaga tcatggcggc cgcggaatcg gagcgattgt tcctgtcgtt
     1261 cctgtcgcac gacgtgcgca atagcctggg gtcggtcacg ctgatgctga aagtgctgcg
     1321 cgatcggctg tcgcgcacca aacagttcgg cgacgaggtc atcgaactgg atacgttgtc
     1381 gcagctgatc tcatcgacga tgagcgggat ggaacggttg ctgcagggcg aatcgttgcg
     1441 gcgcggagcc gtcgcggtag cgcggcaacc gattgacctc cgccgcctgg tggcggacgt
     1501 gacgcgccag cagcagcggc gggcggagga gaagggcatc acgatcctcg tcgaggtccc
     1561 cgaagagatg atcgcgcaca gcgatccgga gctgatcacg ctggtcctgc aaaacctgct
     1621 ggacaacgcg gtgaagttca ccgatcgcgg gcaggtccgc attcggatcg agcgccgggg
     1681 cgacacgttt catctgctgg tgatcgataa cggcccgggc gtgccgccgg aactgctcaa
     1741 ggtcctgtcg cagccgttcc atcgcggtga aaccggcggc cggccgggga tgggattggg
     1801 gttggcgatc gcgactcggg cggcgcaaat gctgggggcg gagctgagga tcgatccacc
     1861 gggcaaagag ggcggggcga cttttcggtt aatcctgccg gcgtaaagag ttcagaagca
     1921 ccagacaaag cccgacaaag cccgtggcta tgccacggga cggttttctc aacccccgtc
     1981 ccacggcata gccgtgcgct tttcttgcgc gcgaatggaa taacgcggcg gtccaaccag
     2041 tctcctctac agaggtgcgc gcgcaatcgc gcgctgcgcc ggcacgcgtg caggagcatc
     2101 gactgttgac gctgaatgag ccgccggatg gcggcgacat ttcgcagact aattccaacg
     2161 ccactcgcgc cgcggtggcg gcggcggtgt tgccgcatct ggacgcggcg ttcaacctgg
     2221 cgcgatggtt gacgcgcgat gaacatgacg ccgccgacgt ggtgcagcag gcgtatctgc
     2281 gcgcgatgac cgccgccgac acgtttcgcg gcggtggggg agccaagtcc tggctgctga
     2341 cgattgtgcg aaacaccgcg ttcgatcggt tgcggcggga taagacgcgc cggcaggaag
     2401 agctgggcga tgatctgccg atgacgccga gtgatgcgga ggcttgcgat ccgcaggcga
     2461 tcctgctgcg tgccgccaac gccgagcgcg tgcgggcggc aatcgaagaa ttgcccgatg
     2521 gcattcgcga agtgatcgtg ctgcgggaga tggaggagtt gtcgtacaag gaagtcgcgg
     2581 ccgtggtggg cgtgccgatc ggcacgatca tgtcgcggct ggcgcgcggc cggcagcggt
     2641 tgcagcaatt gttgagcgaa cccggcgaat cgacc
//
LOCUS       DATIMH010000278         6789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_641640, whole genome shotgun sequence.
ACCESSION   DATIMH010000278 DATIMH010000000
VERSION     DATIMH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641640"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VLI90_04710"
     CDS             complement(<1..550)
                     /locus_tag="VLI90_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSV13536.1"
                     /translation="MATRLIELVESLPRSRVVLIGDLMVDRYLYGNAERLSPEAPVPV
                     LHFQKQELRLGGAGSVAANLAALGSVVSSVGVIGRDEVGQQLRQLLKEIGADGAGLLE
                     CGRPTTCKMRLVGSAQHRHPQQMIRLDFEEPSPIDASLAAAIVERAIAAMDAGAEMIC
                     IEDYNKGVVTADVCRGVIDAASK"
     gene            655..1200
                     /locus_tag="VLI90_04715"
     CDS             655..1200
                     /locus_tag="VLI90_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13537.1"
                     /translation="MAFDTAVTESTERETDGQVAADAHAQFVLQKVQPALLGLMDGSV
                     STLAPLFAAAGVTGKPMYAFLIGTAASVGAGVSMGLAEALSDDGKVTGRGAPVGRGVI
                     TGAATTLGGMLHTLPFLIPNLRYALHTAYAVVVFELMVIAWIRYKFMKTPLAKTIVQV
                     IVGGAIVFFIGIALGKLGAGD"
     gene            complement(1273..2154)
                     /locus_tag="VLI90_04720"
     CDS             complement(1273..2154)
                     /locus_tag="VLI90_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13538.1"
                     /translation="MPDPIQLLLGLGVPMIIAALLAWIGGRAPRGSSWGAIAGLAIGL
                     LIAYRAFLSRWSPLPAIEAPERIVVFNVGLAIAALLLCWQRVPWIARVLLAIAANVLI
                     VWYVFRPMPVSSLPVNRLWTYVAITAAASLALTGLIERIGQRSGSVAAALVLGPFTAG
                     VGLIDFLTGNAKLGWLGASMGLIVFGWFVAAIVSRTTSLARGPIVVCMTALAALLSFG
                     YFVSDSMTTVELLLLAVAPVLGLIAELPPVQRWKPWRRELARLVLVAIPIGVAIGIAV
                     PKFIRENSEATSASQEM"
     gene            complement(2252..2815)
                     /locus_tag="VLI90_04725"
     CDS             complement(2252..2815)
                     /locus_tag="VLI90_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HSV13539.1"
                     /translation="MNTRIASFAAFVGLSVSAAAFAPAAQYKVDPVHSFVNFKVNHLG
                     VSYVWGRFDGPEGTFNTDEGKESFEATVHTDKIDTGVAKRDQHLKSPDFFNAKQFPEL
                     TFKSTGVKKSGENQYEVTGDMTVHGVTKSITVTLTKVGEAKTQMGERAGFDGTFTIKR
                     SDFGMAFMPGAVGDEITVYVGMEGTKE"
     gene            complement(2920..3633)
                     /locus_tag="VLI90_04730"
     CDS             complement(2920..3633)
                     /locus_tag="VLI90_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSV13540.1"
                     /translation="MMQVIQSKDRYFGDHGWLQARWHFSFGDYHDRRNMNWSALRVFN
                     DDVIQPGQGFPAHPHRDMEIVTYVLDGVVRHGDNLGNRGLIKAGEVQVMSAGRGIEHQ
                     EFNGSETEPVHLLQLWIMPRTRGNEPRWEQRSWSGDGTDVRDGVLHPVVSGDGKIPGT
                     LAIDQDAAVYVAKLSPGQDLTHRSEPNRHAYLFVIDGDVTLNGDTTLAKGDQARIADE
                     PALTIRAGSNAHLILLDLP"
     gene            3846..5678
                     /locus_tag="VLI90_04735"
     CDS             3846..5678
                     /locus_tag="VLI90_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13541.1"
                     /translation="MSRLDRHVAFVQGKLALARFLTAVAYTTLAFFALLWLGVVIERL
                     FHVAPPKPTAFAWAGLALALLAAAIYSMLRKPTAHEAAIAIDERLGLKEKFSTALFAR
                     NAQDPFAIAAVRDAERTAENVSLHKRFPLAFPRMGYWSVAGALVVLLTAWLLPTNLDL
                     FGREAQRKHLAEEQAKRDVAKKKVEEALAVVNSYPKTMQNAEAIQIAKRDLEKMLSQP
                     ITDPAQANRTAFKALEQSNEALKEEIKRNEKFAMAQASDKMLRSMNPPNDEQGPVADA
                     QRDIAKGDFSKAVENLNDVANKFDKMSDADQKKAAQQMQSLAKQMEKLAKDPQQMQKL
                     AQQLQQQGMNQQQAQQLAKTMQQAAQGNPQAQQQLQQMQQQAQQAMQKMSPQQQQAMQ
                     NLMKQMQAQSQSQAQAQQMAQAAQQMAQAMQQGASKQGNQQQQGNQQGQMAQAQQAMQ
                     QAAAQMDAVQKDAEQMAAAQDSTAQAQNDAADQGGSQSGNSSGGARPNMGRAGQWRAG
                     ETRGSGNGQGGPGKGMGGGIGKEEAPYAIKKEAAPVQDIANGRILASTFVKAGTVKGE
                     SKVELAKVAKAAEQDMADEVDEEAPSKENQKVVKDYFETMQKSQ"
     gene            5736..6743
                     /locus_tag="VLI90_04740"
     CDS             5736..6743
                     /locus_tag="VLI90_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSV13542.1"
                     /translation="MIRTFITTLVLVALLACTSCSKNAAAPIITVYCAVDEPYASKIF
                     AEFEKETGIRIAALYDIESSKSVGLAGKLEAERDHPQADVWWGSEAFLTARLAEEGVL
                     APYASSNAADIPAVYKDKNGRWAGVGLRARVIALGVPPPDVAIQRLEDLTNPKLKNKI
                     AIARPTAGATGAHVTALYLAWGQDKGDAFFRGLHDNGVALLGGNAEVADQVGAGSFEA
                     GLTDTDDVVNSTANGGKLSMVVPDQGANDAGTMTMPTTVGLVTGSKHAEAAKRLIDYL
                     LSRQTETRLEQLNFVKWSVRDPAGAGGVKAMTVDYAKAAENYAASVRRATALLEGRPI
                     E"
BASE COUNT         1384 a         2048 c         2233 g         1124 t
ORIGIN      
        1 gcttgctcgc cgcatcgatc acgccgcggc agacatcggc ggtgacgacg cccttgttgt
       61 agtcctcgat gcagatcatc tccgcgccgg cgtccatcgc ggcgatcgcg cgctcgacaa
      121 tcgcggccgc gagacttgcg tcgatcggcg acggttcctc aaagtcgagc cggatcatct
      181 gctgcggatg gcggtgttgg gcggagccaa ccagtcgcat cttacacgtg gtggggcgac
      241 cacattcgag taagccggcg ccatcggcgc cgatctcctt cagcaattgc cgcagttgct
      301 gtccgacctc atcgcggccg atgacgccga cggacgagac aactgagccc agcgccgcca
      361 ggtttgccgc gacgctgccg gcgccgccga gccgcagctc ctgcttctga aaatgcagca
      421 cgggcaccgg cgcttcgggg ctgagacgct cggcgttgcc gtagagatag cggtctacca
      481 tgagatcgcc gatcagaaca acccggctgc gggggaggct ttcaaccagt tcgatgagac
      541 gcgtcgccat ggcggcgatt gtacaggaga tggacgggga tttcgtgaca gcgtgactag
      601 gtgaaaccgc aggctatcat tcccggcacg caattaggga gtcccgcaat taccatggcc
      661 ttcgacaccg ccgttactga atccaccgag cgcgagaccg acgggcaagt cgccgcggat
      721 gctcacgcgc agttcgttct ccaaaaagtt cagcccgcgt tgctcgggtt gatggacggc
      781 tcggtctcga cgctcgcccc gctgttcgcc gccgcggggg tgacgggcaa gccgatgtat
      841 gcgttcctca tcggcaccgc ggcaagcgta ggggccggtg tgtcgatggg gttggccgag
      901 gcgttgagtg acgatgggaa agtgacgggt cgcggcgcgc ccgttggccg cggggtgatt
      961 accggcgccg ccacgacgct gggcggcatg ctccatacgc tgccgttttt gatcccgaat
     1021 ctgcgctacg cgctgcacac cgcgtacgcg gtggtggtat tcgagctgat ggtaatcgcg
     1081 tggatccgct acaagttcat gaagacgcct ctggccaaga ccatcgtgca ggtaatcgtc
     1141 ggcggggcga tcgtgttctt cataggaatt gcgctaggga aattgggcgc aggggactga
     1201 gcggcggata gatacaggga tcgttcaatc gcagccgcgc gctaaagcct cgcggctgcg
     1261 atttttcggg gattacatct cttgcgacgc ggacgtggct tctgaatttt cccggatgaa
     1321 tttgggaacc gcgatcccga tcgcgacgcc gatgggaatc gcgacgagca cgagtcgagc
     1381 cagctctcgt cgccagggct tccaccgctg cacaggtgga agttcggcga tcaatccaag
     1441 cacgggcgct acggcaagca atagcagttc gaccgtcgtc atgctgtcgg atacgaagta
     1501 gccgaacgac aacaacgcgg ccagcgcggt catgcagacg acgatggggc cgcgcgcgag
     1561 ggatgtcgtt ctggaaacga tcgctgcaac gaaccagccg aagacgatca agcccatcga
     1621 tgcgccgagc cagccaagct tggcgttgcc ggtcaggaag tcgatcagcc caacgccggc
     1681 ggtgaagggg ccgagcacca gtgccgcggc gacggagcca ctgcgctggc cgatgcgctc
     1741 gatcaatccc gttagcgcca aactcgccgc cgcggtgatg gcgacgtagg tccacaaccg
     1801 gttcacgggc agcgacgaga ccggcatcgg ccggaagacg taccagacga tcagcacgtt
     1861 ggctgcgatg gccagcagca cgcgggcgat ccacggcacc cgttgccagc agagcagcag
     1921 cgcggcgatg gccaagccaa cgttgaagac gacaatgcgc tctggcgcct cgatcgccgg
     1981 cagcggcgac cagcggctta gaaatgcgcg ataggcgatg agcaggccga tcgcgaggcc
     2041 ggcgatggcg ccccatgacg atccgcgcgg ggcgcgcccg ccgatccacg cgaggagggc
     2101 cgcgatgatc atcggcacgc cgaggccgag cagaagttgg atggggtcgg gcatggttgt
     2161 gctcgcggaa atgcaggatg aatgcagaat gcagaattaa gccggatgct caatcctcca
     2221 ttctgcattc atcattcatc attgcgacaa gttactcctt cgtcccttcc atccccacgt
     2281 acacggtgat ctcatcgccc accgcgccgg gcatgaaggc catgccgaag tcgctgcgct
     2341 tgatcgtgaa ggtgccgtcg aaaccggcgc gttcgcccat ctgcgttttg gcttcgccga
     2401 ctttggtgag cgtgacggtg atcgacttag tgacaccgtg caccgtcatg tcgccggtca
     2461 cttcatattg attctcgccg ctcttcttca cgccggtgct cttgaaggtc agctcgggaa
     2521 actgcttggc gttgaagaag tcggggctct tcaggtgctg gtcgcgcttg gcgacgccgg
     2581 tgtcgatctt gtcggtgtgc actgtcgcct cgaacgattc cttgccttcg tcggtgttga
     2641 acgtgccttc gggcccgtcg aatcggcccc agacgtaact gacgccgagg tgattgacct
     2701 tgaagttcac gaaggagtga accgggtcca ccttgtactg ggccgcgggg gcgaaggcgg
     2761 cggcgctgac ggacaggccg acgaacgcag cgaatgacgc gatgcgagta ttcacgatcg
     2821 atctccttga aaggggtttg ttgcgtgtat gtatagccgg ccgcttgcgg cttagcgtgt
     2881 gtttttcgca cgaccgatgc taagccgcaa gcggcaattt tatggaaggt ccaacaggat
     2941 caaatgggcg ttgctgccgg cgcgaatcgt caaagccggt tcgtccgcga tgcgcgcctg
     3001 gtcacctttc gccagtgtcg tatcgccgtt cagcgtcacg tcgccgtcga tgacgaacag
     3061 atatgcgtgg cgatttggct cgctgcggtg cgtcagatcc tgtccgggcg aaagcttcgc
     3121 gacgtagacg gccgcgtcct gatctatcgc caacgtgccg gggatcttcc cgtcgccgga
     3181 gacgacggga tgcaacacgc cgtcgcgaac atccgtgccg tcgccgctcc agctgcgctg
     3241 ttcccaacgc ggttcgttac cgcgcgtccg cggcatgatc cacaactgca agagatgcac
     3301 gggctccgtt tcggagccgt tgaactcttg atgttcgatg ccgcgaccgg ccgacatcac
     3361 ctgcacttca cccgccttga ttagcccgcg gttgccgagg ttgtcgccgt ggcggacgac
     3421 gccgtcgagc acgtacgtca cgatctccat gtcccggtgc gggtgggcgg gaaaaccctg
     3481 gcccggctga atcacgtcat cgttgaagac gcgcagcgcg ctccagttca tgttccggcg
     3541 atcgtggtaa tcgccgaagg aaaaatgcca ccgcgcctgc agccagccgt ggtcgccgaa
     3601 ataacgatct ttcgattgaa tgacctgcat catgagtaat ctcctctgat ccaacgggat
     3661 caacattagc tgttataaca atcattgtca agccaaatat attcccgctg ccggactcag
     3721 ctgaggcaaa gccggatcgg ctgcaaccat tcatgtccct gaccgtcatt aagttattag
     3781 cctgccgcgg tggtggcgat tcactggcgg tcgtacaatt gcgggcagtc aaaagggaag
     3841 ccgttatgag ccgacttgac cgacatgtcg cgttcgtcca ggggaagctg gcgctggccc
     3901 ggttcctgac ggcggtggcg tacacgacgc tggctttctt cgcgctcctg tggctgggcg
     3961 tcgtcatcga acgcctcttc cacgttgcgc cacccaagcc aaccgcgttt gcctgggccg
     4021 gtcttgcact ggccctcctg gcggcggcga tttactcgat gctgcgcaag cccaccgccc
     4081 acgaggcggc gattgcgatc gatgaacgcc tcggactcaa agagaaattc agcaccgcgc
     4141 tgtttgcccg caatgcgcaa gatcccttcg cgatcgcagc ggtgcgcgac gccgagcgca
     4201 cggcggaaaa tgtcagcttg cacaagcggt ttccactggc tttcccgcgg atggggtatt
     4261 ggtccgtcgc gggggcgctg gtcgtcctgt taaccgcctg gctgctgccg acgaacctgg
     4321 atctgtttgg tcgcgaagcg cagcggaagc atctggcgga agaacaggca aagcgcgacg
     4381 tcgccaagaa gaaggtcgaa gaagcgctcg ccgtcgtcaa ttcctatccg aagacgatgc
     4441 aaaacgccga ggcgatccag atcgccaagc gcgatctgga aaagatgctg agccagccga
     4501 tcaccgatcc ggcccaggcg aatcgcaccg cgttcaaggc attggagcag tcgaatgaag
     4561 cgctgaagga agagatcaag cggaacgaaa agttcgcgat ggcgcaggcg agcgacaaga
     4621 tgctgcgctc gatgaacccg ccgaacgacg agcaggggcc ggtggccgat gcgcagcgcg
     4681 acatcgccaa gggcgatttc tccaaggccg ttgagaacct gaacgacgtc gcgaataagt
     4741 tcgacaagat gagcgacgcc gatcagaaga aggccgctca gcagatgcag agcctggcaa
     4801 agcagatgga gaagttggcg aaggacccgc agcagatgca gaagctggcc cagcagctgc
     4861 agcagcaggg gatgaaccag caacaggcgc agcagctggc caagactatg cagcaggccg
     4921 cccagggaaa cccacaggcg cagcagcaat tgcagcagat gcagcaacag gcgcagcagg
     4981 cgatgcagaa gatgtcgccg cagcagcagc aggcgatgca aaacctgatg aagcagatgc
     5041 aggcgcagtc gcagagccag gcgcaggcgc agcagatggc gcaggcggcc cagcagatgg
     5101 cccaggcgat gcagcagggc gccagcaagc aaggcaacca gcagcagcag ggcaatcagc
     5161 agggccaaat ggcgcaagcg cagcaggcga tgcagcaggc ggcggcccag atggacgcgg
     5221 tgcagaagga cgccgagcag atggccgccg cgcaggactc gacggcgcaa gcccagaatg
     5281 acgccgccga ccaaggggga agtcagagcg gcaattcctc aggcggcgcg cggccgaaca
     5341 tgggtcgcgc cggtcaatgg cgcgccggtg aaacccgcgg cagcgggaac ggtcaaggtg
     5401 gtcccggcaa aggcatgggc ggcggcatcg gcaaggaaga agcgccgtac gcgatcaaga
     5461 aggaagccgc cccggtgcag gacatcgcca acggccggat cctcgccagc acgttcgtca
     5521 aagccggcac cgtgaagggc gagtcgaagg tggagctggc caaggtcgcc aaggccgccg
     5581 agcaagacat ggccgacgag gtggacgaag aagcgccgtc gaaggaaaat caaaaggtgg
     5641 tcaaagatta tttcgagacg atgcagaagt cgcagtaatt cccccgcgcg gcctggtcac
     5701 tttagtgacc gggctccccc agtctgagcc aagacttgat ccgcacattt atcaccactc
     5761 tggttctcgt cgcgctcctc gcctgcacca gttgctccaa gaacgccgcc gcgccgatca
     5821 tcaccgtgta ctgcgcggtg gacgaaccgt acgcgagcaa gatcttcgcc gagttcgaaa
     5881 aagaaaccgg catccgcatc gccgcgctgt acgacatcga atcaagcaag tcggtgggcc
     5941 tggcgggcaa gctcgaagcc gagcgcgatc acccgcaggc ggacgtctgg tggggcagcg
     6001 aagcgtttct caccgctcga ttggctgaag aaggagtgct cgcgccgtac gccagctcca
     6061 acgccgccga cattccggct gtctataagg acaagaacgg ccgatgggcg ggcgtgggcc
     6121 tgcgggcgcg ggtgatcgcg ctgggcgtgc cgccgccgga tgtggcgatt cagcggctgg
     6181 aggacctgac gaacccgaag ctgaagaaca aaattgcgat cgcgcgaccg accgccggcg
     6241 cgaccggcgc gcacgtcacg gcgctgtatc tggcctgggg acaggacaag ggcgacgcct
     6301 tcttccgtgg gttgcacgac aacggcgtcg cgctgctcgg cggcaacgcg gaagtcgccg
     6361 atcaagtcgg agccggttcg tttgaagcgg gactgaccga cacggacgac gtggtcaact
     6421 ccaccgccaa cggcggaaag ctgtcgatgg tcgtgccgga ccaaggcgcc aacgacgccg
     6481 gcacgatgac gatgccgacg acggtcggcc tggtcaccgg cagcaagcac gcggaagcgg
     6541 ccaagcggtt gatcgactat ctcctgagcc ggcagaccga aacgcggctg gagcagctga
     6601 acttcgtgaa gtggagcgtc cgcgaccccg ccggcgccgg cggcgtgaaa gcgatgacgg
     6661 tcgattacgc caaagcagcg gagaactatg cggcaagcgt gcggcgcgcg acggcgctac
     6721 tggaaggacg gccgatcgag tgaacgaggt cagaccttga tagccggggg cggaagcccc
     6781 ggtctgttc
//
LOCUS       DATIMH010000279         4489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2370868, whole genome shotgun sequence.
ACCESSION   DATIMH010000279 DATIMH010000000
VERSION     DATIMH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2370868"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..659
                     /locus_tag="VLI90_04745"
     CDS             222..659
                     /locus_tag="VLI90_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HSV13543.1"
                     /translation="MTTPPAISDAEWEVMNVLWDAGPQSLSAAEVVDRIAASNNWSPR
                     TVKTLLNRLIKKGALTFTRDGKSYLYRPAVRREQCVRAVSRSFLWRVFGGNVAPMLAH
                     FVTHAELSAEEVDQLQRLLHEKRAPADAAAAAAAADKPHRPTH"
     gene            670..3144
                     /locus_tag="VLI90_04750"
     CDS             670..3144
                     /locus_tag="VLI90_04750"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HSV13544.1"
                     /translation="MSNVLVHVWQNSWQAAIVALLVLVMRAMFGRVLTRRRCHALWIL
                     VLLRLALPVLPGSPVSLFEIVHRFWQQTPPRAAEQSDLKWTVLYGPMSNTRAPAIADQ
                     PRQTSTQTNRQAPVDLLSFTWLAGVALFAARAIYGNAVFMRRLRRRPAADADEPTRQL
                     FRDCCNAMRLRRPPQLLMTDAITSPAVAGLFRPRVLLPVGLTADLTTEQLRLVLLHEL
                     AHVRCRDVAVDWLWAAVQAMHWFNPIVWLIRPLRHADREQARDEMVLSIVGARQAECY
                     GRTILGFVQSARPIRLYPGLIGILNKRAGLGKRIEMIARFQQRSLVLRLLGLTMFIAV
                     GCIALTNPPTTHAQAQTPAPGNTIATPPSTRPAISPAAEPNESAADAVVRDKLDRRMN
                     ALNFDKVPFADVIDFFRQSMDLNIYVDWPGLERASIKRDAPVTARLRDIRFSKALELI
                     FKCVEGDEDDRKIGYVIDDGVLTISTHRELNKNVVTERYDINDLLFVAPDYSNAPPST
                     TAPTADEQHNLRQQRIEELKKFIVDNVATTSWKINGGEVGSISSSPLRAMLLITQTPS
                     NHRAIHQLLDSLRESQSRQISVESRLFLLDDAAERALSDNLRQHLTAAARADKDSSTN
                     VLPAAQVDELIRVIRKWQGATSLTAPRLTLFSGQRAVLSVSTSTTYIASMSANVKTTG
                     NSVVTTYEPQNSNVTGTGIKVDVMATVAPDNRGVYVDFHPVLAQLDRLDSVPWAGAPA
                     DAHVFVQKPIVSTCELRTSCTIPDGASVVLGGFSQSTPATADAAVQNDVERPTTNPDA
                     MASLVSASRSGRLYWLIKPTILPHVK"
     gene            3341..>4489
                     /locus_tag="VLI90_04755"
     CDS             3341..>4489
                     /locus_tag="VLI90_04755"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSV13545.1"
                     /translation="MATAAQPAPKKIVLAYSGGLDTSVILPWLKERYPGVKLVAFAAE
                     LGQGSELEGIEAKAKKSGADEVVVMDLREEFAEQYCFPMLRAHAIYEQDYLLGTSIAR
                     PLIAAKQVEVARQTGADAVGHGATGKGNDQVRFELTYMALAPDLKIIAPWKDPSFELR
                     DRESAIEYAEKHGVPIPVSKTKIYSQDRNLWHISHEGAEIEHPDQEPNWLKCLTMTTP
                     PEKAPNESEVVEVGFERGNPISVNGKKLPPGELIAELNKIGGKHGVGITLLVENRLIG
                     MKSRGVYETPGGTILYEAHKALEQICIERDTLHYKQQVALRYAELVYYGQWFHPLREA
                     LQTFIDHTNATVTGTAKVRLYKGKAQPIAAGSPHSLYDSKLASFSMEGY"
BASE COUNT          910 a         1506 c         1302 g          771 t
ORIGIN      
        1 cgccgggttc ggagcgtact cgcgacgacc cggatttgtc ggattctgtc gattgtgtcg
       61 gattctgtgg gatcgccggt cggttgtgtc ggattctgtt ggattctgtc ggattgtgtt
      121 ggatcggtag aaggatccgg cccatcgcgc ttggttccag ccaagcctgc cggtgcctcc
      181 ttgacatccg attacaagtg taatagatta cacccgtaat cgtgaccacc ccgcccgcca
      241 tctccgacgc tgaatgggaa gtgatgaacg tcctatggga cgccggtccg cagtcgctta
      301 gcgctgccga agtggttgac cgaatcgcgg catccaacaa ctggagcccg cgaacggtca
      361 agacgctgct caaccgcctg atcaagaaag gcgccctgac cttcacgcgc gacggcaagt
      421 cctatcttta ccgccccgcc gttcggcgcg agcagtgcgt ccgcgcggtg agccgctcat
      481 ttctctggcg cgtcttcggt ggcaacgtcg cgcccatgtt ggcgcacttc gtcactcacg
      541 cggaattgtc cgcggaggaa gtggatcagc tgcagcgcct gctgcacgag aagcgcgcgc
      601 ccgccgatgc cgccgccgcc gccgccgccg ccgacaaacc gcatcgaccc acccactaag
      661 gattgcgcca tgtcgaacgt gcttgtgcat gtctggcaga acagctggca agcggcaatc
      721 gtcgcgctgt tggtgttggt catgcgcgcg atgttcggcc gcgtgctcac ccgccgacgg
      781 tgccatgcgc tgtggatact cgtactgctt cgcctggcgc tgccggtgct gccgggcagc
      841 ccggtcagcc ttttcgaaat cgtccaccgc ttctggcagc agaccccgcc acgagccgcc
      901 gaacaatccg atctaaagtg gacggtgctt tatggcccga tgagcaacac gcgcgcgcct
      961 gcgattgcgg accagccgcg gcagacatcg acacagacaa accgccaagc gccggtcgat
     1021 ctgctctcgt tcacctggct cgctggcgtc gccctgtttg ccgcgcgggc gatctacggc
     1081 aacgccgtat tcatgcgccg gctgaggcga agaccggccg ccgatgcgga tgaaccaacg
     1141 cgacaactct tccgcgattg ctgcaacgcg atgcgtctgc ggcgcccacc gcagctgctc
     1201 atgacggacg cgatcaccag ccccgcagtc gcaggattat tccggccgcg cgtgctgctg
     1261 ccggtcggcc tcaccgcgga tctgaccacc gagcagcttc gcctggtcct gctgcacgag
     1321 ctggcgcacg tgcgctgccg cgacgtcgcc gtcgattggc tgtgggccgc cgtgcaggcg
     1381 atgcactggt tcaacccgat cgtctggctg atccgcccac tgcgccacgc cgatcgcgag
     1441 caggcgcgcg atgagatggt gctgtcaatc gttggagcgc gccaagccga gtgctatggc
     1501 cgcaccattc tcggtttcgt tcaatccgcc cgccccatcc gcctctatcc gggcctgatc
     1561 ggaatcctca acaagcgcgc aggcctcggc aaaaggatcg aaatgatcgc ccgttttcag
     1621 caacgctctc tggtccttcg cctgcttggt ttgacgatgt tcatcgccgt cggctgcatc
     1681 gcgttgacca acccgccgac cacccatgcc caagcccaaa cccccgcacc ggggaacacc
     1741 atcgcgacac cgccgtcaac acggccggcc atttcccccg cggcggaacc caatgaatcc
     1801 gcagcagacg ccgtcgttcg cgacaagctc gatcgacgca tgaacgcgct taacttcgac
     1861 aaggtgccgt tcgccgacgt catcgacttc ttccgccaaa gcatggactt gaacatctat
     1921 gtcgattggc ccgggctcga gcgcgcctcc atcaagcggg acgcgccggt caccgcccgt
     1981 ttgcgcgaca tcagattctc aaaagcactg gaactgattt ttaagtgcgt cgaaggcgac
     2041 gaagacgacc gcaaaatagg ttacgtcatc gacgacggcg tcctcaccat cagcacccac
     2101 agggagctga acaaaaacgt cgtcaccgaa cggtatgaca tcaacgatct gctgttcgtc
     2161 gcgcctgatt attccaacgc cccgccgtcg accacagcgc ccaccgccga cgaacagcac
     2221 aacctgcggc agcagcgcat cgaagagcta aaaaagttca tcgtcgacaa cgtggcgacg
     2281 accagttgga agatcaacgg cggcgaagtc ggctccatca gctccagtcc cctgcgcgcc
     2341 atgctgctga tcacgcagac cccctcgaac caccgcgcaa tccaccagct tctggattca
     2401 ctgcgcgagt ctcaaagccg gcagatttct gtcgaaagcc ggctgttcct cctcgatgac
     2461 gccgcggagc gcgcgttgtc cgacaacctg cgccagcacc tgaccgccgc cgcccgcgct
     2521 gacaaggaca gctcgacaaa cgtgcttccc gccgcacagg tggacgagct cattcgcgtc
     2581 attcggaaat ggcagggcgc caccagcctg accgcgcctc gactcacgtt gttcagcgga
     2641 caacgggcag tcctgtctgt gtccacttca acgacctaca tcgccagtat gagcgccaac
     2701 gtgaaaacga ccggcaattc cgttgtcact acatatgaac cccaaaacag caacgtgacc
     2761 ggaaccggca tcaaggtcga cgtgatggcg accgtcgccc cggacaaccg cggcgtttac
     2821 gttgacttcc acccggtcct cgcccaattg gaccgcctcg actccgtacc ttgggccggc
     2881 gcgccagcgg atgcacacgt cttcgttcag aagccgatcg tttcgacgtg cgagcttcgc
     2941 accagctgca cgattcccga cggagcgtcg gtcgtactcg gcggcttcag ccagtccaca
     3001 ccggcgaccg ccgacgcggc agtccaaaac gatgtcgagc gcccgacgac caatccggac
     3061 gcgatggcgt cgctggtttc cgccagccgc agcggaagac tttattggtt gatcaaaccg
     3121 acgatcctgc cgcacgtgaa gtgatcgcgt gaaaaaaatc tgcccgcgta gcggtcgccc
     3181 cgaaggggca cgcgtttttt cctcgaatgg aaaagaccgt gccccttcgg ggcgaccgct
     3241 acgcagcatc tcgcacattc gtggcgcgcg tggcgaagaa ttcgcccatc cctatactgt
     3301 cccgccgaac ttgaatttct tgaatcggag tcccacacac atggccacag cagcgcaacc
     3361 cgcaccgaag aaaatcgtcc tcgcgtacag cggcggactt gatacgtccg tcatcctccc
     3421 ctggctcaaa gagcgttacc ccggcgtcaa gctcgtcgcg ttcgcggcgg agctgggcca
     3481 aggcagcgaa ttggaaggca tcgaagccaa ggcgaaaaag agcggcgcgg atgaagtcgt
     3541 cgtgatggat ttgcgcgagg agttcgcgga acaatattgc ttcccgatgc tgcgggcgca
     3601 cgcgatttac gagcaggatt acctgctcgg cacgagcatc gcccggccgc tgatcgcagc
     3661 caagcaagtc gaagtcgccc ggcagaccgg cgccgacgcc gtcggccacg gcgcgaccgg
     3721 caagggaaat gaccaagttc gatttgagct gacctacatg gcgctggcgc cggatctgaa
     3781 gatcatcgcg ccgtggaagg acccgagctt cgagctgcgc gaccgcgaga gcgcgatcga
     3841 atacgccgag aagcacggcg tgccgatccc ggtgagtaag acgaagattt atagccagga
     3901 ccgaaatctg tggcacatca gccacgaagg cgcggagatc gagcacccgg atcaggagcc
     3961 gaactggctc aaatgtttga caatgacgac gccgccggag aaagcgccga atgaaagtga
     4021 agtcgtcgag gtcggcttcg agcgcggcaa cccgatctcg gtcaacggca agaagcttcc
     4081 gccgggtgag ttgatcgcgg aactgaacaa gatcggcggc aaacacggcg tcggcatcac
     4141 attgctcgtc gagaaccgcc tgatcggcat gaaatcccgc ggcgtctacg agacgcccgg
     4201 cggcaccatc ctgtacgaag cccacaaggc gctcgagcaa atctgcatcg agcgcgacac
     4261 gttgcactac aagcagcagg tcgccctgcg ctacgcggag ctggtctact acggccaatg
     4321 gttccacccg ctgcgcgagg cgctgcagac gttcatcgat cacaccaacg ccaccgtcac
     4381 cggcaccgcg aaggtgcggc tgtacaaagg caaggcccaa ccgatcgccg ccggcagccc
     4441 gcacagcctg tacgacagca agctagccag cttcagcatg gaaggctac
//
LOCUS       DATIMH010000280         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4684972, whole genome shotgun sequence.
ACCESSION   DATIMH010000280 DATIMH010000000
VERSION     DATIMH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4684972"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..>2044
                     /locus_tag="VLI90_04760"
     CDS             171..>2044
                     /locus_tag="VLI90_04760"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HSV13546.1"
                     /translation="MTQLAHHPALLQIEDRLDAAGQKYRGQRIVRGAILWLATAIAAS
                     WSAALAAHFIGAVSVGGSVWTYLVLAIWLAVIIAATVVWIVRPLFIRPAAVEVARLLE
                     SRVPGLHNGLTNGVLLARAEDLHDSPWLPQIFEEIHQTIAREPIGDAVRLADLRPVFV
                     RAMLIALPLLLVSGIFPRPFVHGWQQMLHPAAFVPQVAATTHIIDVQPGDVTLVRDQP
                     LEIMATATGSAAQDAKLIFDNGSPSASMPGMASDSGTRYSYRVEHVDQSFKYRLEIGG
                     TQSRWYSVNVVRQILLQQVTLKITPPAYTRKVSSVLALKTVEIAATPITVPQGSAIEL
                     TEGIDIAANAAMFQIGEQPPTPMTANTTHTAFSTSLTIVDDVTAAMLLTDGAGQVVAK
                     LPADSLAIHCSKDAAPAIEMRWPAHDAVVAPADELKVQATIHDDYGVTSARVLLSANA
                     TDPLAPVATFNYPASSDAVDLASVLDLKPEVRKHGNAIRVQVQATDNRDLGAGNGPQT
                     TASPIIEIKFRDPETIAKEERESSDKLRTILWQMLKTQQALHAQTIAWKPAAADVMRK
                     ISSGQSSLRETMRSTAETFTFDASNQIIQKTLLVLSLNPAKDAVDQSAALLKAIDPKE
                     "
BASE COUNT          364 a          649 c          689 g          342 t
ORIGIN      
        1 actacccgac ctggtcaatg ccgaccccac cgccttcgcc gcccgcgtgg tgcggcggac
       61 acgccgccac cccacccgcc ccgctaaggc cgcctgagcg ccagcgcggg catcgattcg
      121 acggtatact gatatgggac cgctgcgccg ccgcgcacga gtctctccgc atgacgcaac
      181 tggctcacca tcccgccttg ctgcagatcg aggaccggct cgatgccgcg gggcagaagt
      241 accgcgggca gcgcatcgtg cgcggggcga tcctgtggct ggcgacggcg atcgccgcca
      301 gctggtccgc ggcgttggcg gcgcatttca ttggcgcggt ttccgtcggt gggagtgtgt
      361 ggacgtatct ggttctggcg atctggctcg cggtcattat cgccgcaacg gtcgtatgga
      421 tcgtccggcc gctgttcatc cgccccgccg ccgttgaagt ggcgcggctg ctcgaatcgc
      481 gcgtgccggg acttcacaac ggtctgacca acggcgttct gctcgcgcgc gccgaagacc
      541 tgcacgacag cccttggttg ccgcagattt tcgaggagat ccaccagacg atcgcgcggg
      601 agccgattgg cgatgccgtt cgcctggctg atcttcggcc ggtcttcgtg cgcgcgatgc
      661 tcatcgccct gcctctgcta ctcgtctctg gcattttccc gcggccgttc gtccatgggt
      721 ggcagcagat gctgcatccg gcggcgttcg tgccgcaggt ggcggcgacg acgcacatca
      781 ttgatgttca acctggcgac gtgacgctcg tgcgcgatca gccgctggag atcatggcga
      841 ccgccaccgg atcggccgcg caagatgcga agctgatctt cgacaacggc tcacccagcg
      901 catcgatgcc ggggatggcg agcgacagcg gcacgcgcta ttcgtaccgc gtcgagcacg
      961 tcgatcaatc gttcaagtat cgtctggaga tcggcggcac gcagagccgg tggtacagcg
     1021 tcaacgtcgt ccggcagatt ttgctgcagc aggtcacgct gaagatcacg ccgccggcgt
     1081 acacgcgcaa ggtgtcgtcg gtgttggcgc tgaagacggt ggaaatcgcg gcgacgccca
     1141 tcacggtgcc gcagggcagc gcgatcgagc tgacggaggg catcgatatc gcggccaatg
     1201 cggcgatgtt tcagatcggg gagcaacccc cgacgccaat gacggcgaac acgacgcata
     1261 cggcgttcag cacgtcgctg acgatcgtgg acgacgtgac ggcggcgatg ctgctgacag
     1321 acggggccgg ccaggtcgtc gcaaagctgc cggcggattc gctggcgatc cactgcagca
     1381 aagatgccgc tccggcgatt gagatgcgct ggcccgcgca cgatgcggtc gtcgcgcccg
     1441 cggatgaact gaaggttcag gcgacgattc acgatgacta cggcgtgaca tcggcgcgcg
     1501 tgctgctgtc cgcgaatgcg accgacccgc tcgcgccggt ggcgacgttc aactatcccg
     1561 ccagctctga cgcggtggat ctggcatcgg tgctagacct caagccggag gttcgcaagc
     1621 acggcaacgc gatccgcgtg caggtgcagg cgacggacaa tcgagacctc ggcgccggca
     1681 acggaccgca gacgacggcg tcgccgatca tcgagatcaa gttccgcgat ccggagacga
     1741 tcgcgaagga agagcgggaa tcgagcgaca agctgcgaac gattttgtgg cagatgctca
     1801 aaacgcagca ggcgctgcac gcgcagacga tcgcatggaa gccggcggcg gcggatgtga
     1861 tgcggaagat cagcagcggg cagtcctcgc tgcgcgagac gatgcgttcg accgcggaga
     1921 cgttcacctt cgatgcgtca aatcagatca tccagaagac gctgctggtg ctgtcgctga
     1981 acccggcgaa ggatgcggtg gatcagtcgg ctgctctgct caaagcgatc gatccgaagg
     2041 aaca
//
LOCUS       DATIMH010000281         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4103796, whole genome shotgun sequence.
ACCESSION   DATIMH010000281 DATIMH010000000
VERSION     DATIMH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4103796"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VLI90_04765"
     CDS             complement(<1..553)
                     /locus_tag="VLI90_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HSV13547.1"
                     /translation="MTTSTRSAPIRPLSLSVPANAPASPVGDHPHSTSLDLAIALATD
                     ALLAKQAPGGWWVGELQGDSILESEYILLKWIINQEADPDLPLIANYLRNLQNADGGW
                     SLYPGGPPDISGCVKAYFALKLMGDDPSAPHMTKARQLILSLGGAEKCNTFTRFYFAC
                     LGQISFDSCPSIPPEVVFLPKWFY"
     gene            859..1422
                     /locus_tag="VLI90_04770"
     CDS             859..1422
                     /locus_tag="VLI90_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13548.1"
                     /translation="MAQRTLRCNIEDIAKWHPWFYLEPHIVSCAAVLARYGPAPALFD
                     VECTRVKSTWLGDARRFRLEIGWSNETERKADRLRATVQPKPLVEMAATAVALVLSHR
                     VLALGQLDVTRYGERTDFRSVAVDCMLEISGTEMPDHLGRRQREKIAQAMTNPLGWDS
                     YVVVCAFFQRGHRVRLSFHSPEGSTHG"
     gene            1415..1789
                     /locus_tag="VLI90_04775"
     CDS             1415..1789
                     /locus_tag="VLI90_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13549.1"
                     /translation="MAKAKPYTKQVKQLMAEKSRLLSKAEAFEQMNLGETAQPLWATV
                     AASEERLAPLLDLLGRESEAALHRISAASAYERCGQPGRAANLYRAALAGPLDDAARK
                     DVGKKLTACLKQLQRDATQSVA"
     gene            complement(2546..>2833)
                     /locus_tag="VLI90_04780"
     CDS             complement(2546..>2833)
                     /locus_tag="VLI90_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13550.1"
                     /translation="VWHLFTDNGETKLVRVDQPDAMNIKTTAVTITNDLPQEFYDGRV
                     RFVLDRGRYRTAKNGTLLAQYDCEDNTKTAVLVKVDIPASGSTTVTVEAER"
BASE COUNT          587 a          852 c          864 g          530 t
ORIGIN      
        1 agtaaaacca cttgggcaga aacacgactt ccggcggaat gctcgggcac gaatcgaagc
       61 tgatctgccc caggcacgcg aaataaaacc gcgtgaacgt gttgcacttt tccgccccgc
      121 cgaggctaag gatcaactgg cgcgcctttg tcatgtgcgg cgcgctcggg tcatcgccca
      181 tcagcttcag cgcgaagtat gccttaacgc atccggaaat gtccggcggc ccgccggggt
      241 acaagctcca cccgccgtcg gcgttctgca ggttgcgcag ataattcgcg atcagcggca
      301 ggtccgggtc ggcttcctga ttgatgatcc acttcagcag gatgtattcg gattcgagga
      361 tgctgtcgcc ttgcaactcg cccacccacc acccgccggg agcttgcttg gccagcaacg
      421 catcggtcgc gagcgcgatc gcaagatcca gcgaagttga atgagggtga tcacccaccg
      481 gcgatgccgg ggcgtttgcg gggactgaga gactcagcgg ccgaatcggc gcgctgcggg
      541 tgctggtcgt cacgacggat acatcctctg atgaagaaca gggttgagcc aaatcgcggc
      601 cctgcggaac cggggcgaaa acgtaccacg ggggcgggag ttggacaagg ctgggggtgg
      661 gcgggtcgcc gaaggcattt gaaacaacac ggcgagctaa tgggcataag ttgctggccc
      721 gcgcatggaa ctcgatgcta tgcgccgcct ggctttgaca gtcccgggct gaagcggtca
      781 aatccgtcac taagcgagat tcccccgcgg cgcatctctc ggcgtcggcg acggcaatgg
      841 gctccgcggc ggttctatat ggcccaacgg accctgcgct gcaacatcga agatatcgcc
      901 aagtggcatc cgtggttcta tcttgagccg cacatcgtct cgtgcgccgc cgtgctggct
      961 cgctatggcc ctgcgccggc gctctttgac gttgaatgca cccgcgttaa gtccacttgg
     1021 ttaggtgacg caaggcgatt tcgtctcgaa atcgggtggt caaacgaaac cgagagaaaa
     1081 gcagatcgcc tgcgtgccac cgtccaaccc aaaccgctcg tcgaaatggc cgcgacagcc
     1141 gtggcgctcg tcctttcgca ccgggtgctt gccctcggtc agctggacgt cacgcgctat
     1201 ggcgagcgaa ctgatttccg atcagtagcc gttgactgta tgctggaaat cagcggcacg
     1261 gagatgcctg accatttggg ccgccgacag cgcgagaaga tcgcacaagc gatgaccaat
     1321 ccgctgggtt gggacagtta tgtcgtcgtg tgcgcttttt tccaacgagg gcaccgcgtc
     1381 cgtctgtcct tccatagccc cgagggatcg acccatggct aaagcgaaac catacaccaa
     1441 gcaggtcaag cagctgatgg ccgagaagtc gcggctgctc agcaaagcgg aggcgttcga
     1501 gcaaatgaac ctgggcgaga cggcccagcc gctgtgggcg acagttgccg cgtctgaaga
     1561 gcgtctcgcg ccgctgctgg accttctggg gcgggaatcg gaagcggcac tgcaccgcat
     1621 cagcgctgcc agcgcctatg agcgctgcgg tcagccgggc cgcgccgcca atctctaccg
     1681 cgccgcgctg gccggcccac tcgacgacgc cgcgcgcaaa gacgtgggga agaagctgac
     1741 ggcctgtctt aaacaactgc aacgggacgc aacccagtcg gtcgcataac tcgccggaca
     1801 catcagccaa tcggcagacg attcgcacat tggcgtcgcg ctacggtcgt atagaatgac
     1861 gcggtccagc cgaggatcgt gaccgcattc ttcgacccgc gtctgaggcg aaagcgatga
     1921 gcaaacgcaa atcggtcaaa ttcgaatatg gccgcgagag cgatgctgct tacctgcgca
     1981 tttgaccagg caagtccgcg agtccgaaga agtcgagccc ggcctgatcg tggatctggg
     2041 cgcggacgat caagtcatcg gtgtcgaaat cctacgcttc acccgccgct tcgggcgagg
     2101 gacgagaaac tcgcgggata accgcagcgg tgacgcatgc tcaacgttct gcacaacagg
     2161 aaaagcgaag tgcaggacga agaaaaatgt tgcgcgtcac cttgttcgct tccgagcctg
     2221 cagtgccttt atgaaaaacg ccaacgtgag atttatggct cagtcgtcgg ctgtatcttt
     2281 ttcaggagag cgcgaaccgc aggtcggcac tctccgctga ccaactcggg gaggctaatt
     2341 tagcgaaatg ccatctgaac ccgtcagcaa agtcaagtat aaagaacgag gaggtggatg
     2401 acgccggctg cttgcgccgt tggtgattta cgaaaacatc gtcaatccgg tcgggacggc
     2461 tcggacgtgt cctccagcat tcaaaaagct tccattccct ggcgaccgtg aacgggcgcg
     2521 gcttgttcag aacagccacg acgctttatc gttccgcttc aaccgtcacc gtcgttgacc
     2581 cacttgcggg gatgtccacc ttcaccaaca ccgccgtctt agtgttgtcc tcgcaatcgt
     2641 actgcgccag cagcgtgcca ttcttagccg tgcgatagcg gccgcgatct agcacgaagc
     2701 gcacgcggcc atcgtagaac tcctgcggca gatcgttggt gatcgtcacc gcggtcgtct
     2761 tgatgttcat cgcgtccggc tggtcgacgc gcaccaactt ggtttcgccg ttgtcggtga
     2821 acaggtgcca gac
//
LOCUS       DATIMH010000282         4751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2950443, whole genome shotgun sequence.
ACCESSION   DATIMH010000282 DATIMH010000000
VERSION     DATIMH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2950443"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VLI90_04785"
     CDS             complement(<1..433)
                     /locus_tag="VLI90_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSV13551.1"
                     /translation="MSESQHSGHILITGASTGIGRTTAIHLATIGFHVFAGVRRETDG
                     QSLEADGGGRITAVRIDVADDASIASAFEMIRTTVADDGLLGVVNNAGIGVFGPIEHL
                     SRDEWHRQFNVNVFGPIAVTQAALPLLRAHVQRHGRGSARLV"
     gene            592..1998
                     /gene="lpdA"
                     /locus_tag="VLI90_04790"
     CDS             592..1998
                     /gene="lpdA"
                     /locus_tag="VLI90_04790"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HSV13552.1"
                     /translation="MAEQPSQYDLVIIGAGPGGYVAAIRAAQLGMKVACIDRQFLGGT
                     CLNVGCIPSKAMLESTERFYETKSKLAHHGIKVDGVSLDLPQMLARKDAVVKQLTSGV
                     GFLFKKNKIDSIIGHGRITARDTVEVKARDGKATTLKAKRIMIATGSAPIELPGLKFD
                     GKYIVDSTGALSFSEVPKKLVVIGAGYIGVEMGSVWRRVGSEVLFIEFTPTILPLMDQ
                     ELAGLLQRSLEKQGMKFKFNAAAQGAQVKDGEVFVTVKSGDQTTVEECDRVLVSTGRK
                     PITDGLGLAELGVEMDKRGFVKVNDHYETNIPGIYAIGDVIGGLMLAHKAEEEGIAAA
                     ELMAGKAGHVNYKVVPGVVYTHPELAQVGLTEADAKKEGYEVRVGKFPLKANGRAISI
                     DDTEGWMKVVGDAKTDRLLGVQILSPHASDIIAEACVAMEFAASVEDLARTMHAHPTL
                     PEALKEAALAAEKRAIHI"
     gene            2178..2720
                     /locus_tag="VLI90_04795"
     CDS             2178..2720
                     /locus_tag="VLI90_04795"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HSV13553.1"
                     /translation="MAATDCPALIAVFDSRDEAELAVDELREAGFRDDEIGIVLRGRD
                     AIAGGMITDAEGTKDAVGAVEGAATGAVIGAGFGALVTLLIPGAGPVLAAGILATALG
                     YAGAGAAIGGIYGAMKGLGVSEEEAQRYEEQFNAGKAIVAVKAGARLPDAIGIFRRHG
                     GFDLHQDVNNPVPLTGPNEF"
     gene            2759..3538
                     /locus_tag="VLI90_04800"
     CDS             2759..3538
                     /locus_tag="VLI90_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13554.1"
                     /translation="MRSHLIENTMSLLGGAGLGAALMYLFDPDLGEQRRQDARQVAGQ
                     AVASTGDAVQSTVHTATDSARSFASCISDYAHDLADRAGGQSSNLTDRAGDLASGAMK
                     SARKMWNRSSSSAGDYASDAADRARGVRDDLSDRASSLWSSARQSSGMEESHPIATAT
                     GITAGTIGVLALGAGLMYFMDPERGRGRRAWASDKIFSVSRRTGKRARSLGRHLGNRM
                     QGVAHEAMNRVPENWTGGGSGESSQATSGEPQQQSMPSAGM"
     gene            complement(3686..>4751)
                     /locus_tag="VLI90_04805"
     CDS             complement(3686..>4751)
                     /locus_tag="VLI90_04805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HSV13555.1"
                     /translation="RKAAFQQGLSALKAGRSNKLTRKTGPVGAIRPTLYNPAGMPNTN
                     QIIQGDSIKVLNEGPERWVDLVFADPPFNIGYLYHGYNDELKAEDYLKFSTDWMAAVH
                     RALKPTGSFYLAIGDEYAADLAVIARRQLGFNLRNWIIWHYTFGQQTKFMFAKSHTHI
                     FYFTKSKEGFTFNPDAVRVASARQTTYGDHRANPKGKLPDDTWYLRPQEAGGNGYFEP
                     GHDVWNESRVCGTFKEREGWHGCQMPIGVLNRIIKASSNPGDVVLDPFNGSGTTCVSA
                     ALLGRQYVGIDQSEEYVGYARKRLEHALAQKVTADGEDHTAIAAVIDPNRESKVMTKT
                     DAFGRPRVARGRRRTIAETA"
BASE COUNT          866 a         1456 c         1586 g          843 t
ORIGIN      
        1 tcacaaggcg cgcgctgccg cggccgtggc gctgcacatg cgcgcgcagc agcggtagcg
       61 ctgcttgagt caccgcgatg ggaccgaaga cgttgacgtt gaactggcga tgccattcgt
      121 cgcgcgacaa gtgttcgatg ggcccaaaga cgccgatgcc ggcgttgttg acgacgccga
      181 gcaagccgtc atcggcgacg gttgtgcgaa tcatctcgaa ggcgctggcg atcgacgcgt
      241 catcggcgac gtcgatgcga acggctgtga tgcggccacc gccgtcggct tcgagggatt
      301 ggccatccgt ctcgcggcga acgccggcga agacgtggaa gccgatggtc gcgagatgga
      361 tcgcggtcgt ccggccgatg ccggtgctgg cgccggtgat gagaatgtga ccagagtgtt
      421 gggattcgga catatccgcg agtgataccc ccacgccggg ttcggaggta ctccgactgc
      481 ccggattctt ctcgcgccgc agaatctcgc ttcgtcgcga gtacgctccg aacccggcgt
      541 ggtaagcgcc tctacccttg cgcgaactcc gtccctaata tgtctcgacc tatggctgaa
      601 caaccctcgc aatacgacct cgtcatcatc ggcgccggcc ccggcgggta cgtcgcggcc
      661 atccgcgcgg ctcagctggg catgaaggtc gcctgcatcg accggcaatt cctcggcggc
      721 acgtgcctga acgtcggctg cattcccagc aaggcgatgc tcgaatcgac cgagcggttt
      781 tacgaaacga agtcgaagct cgcccatcac ggcatcaagg tggacggcgt ttcactcgac
      841 ctgccgcaga tgctggcccg caaggatgcg gtggtgaagc agctgaccag cggcgtcggt
      901 ttcctgttca agaagaacaa gatcgattcg atcatcggcc acggacgcat caccgcgcgc
      961 gacaccgttg aagtgaaagc acgggatggc aaggcgacga cgctgaaggc gaagcgcatc
     1021 atgatcgcca ccggcagcgc gccgatcgag ttgccgggat tgaagttcga cggcaaatac
     1081 atcgtcgatt cgacgggcgc gctgtcgttc agcgaggtgc cgaagaagct ggtcgtgatt
     1141 ggcgcgggtt acatcggcgt cgaaatgggc agcgtctggc gacgcgtcgg cagcgaggtg
     1201 ctgttcatcg aattcacgcc aacgattttg ccgctgatgg atcaggagct ggcggggctg
     1261 cttcagcggt cgctcgaaaa acaggggatg aagttcaagt tcaacgcggc cgcccaaggc
     1321 gcccaggtga aggacggcga ggttttcgtc accgtcaaga gcggcgacca gaccaccgtc
     1381 gaagaatgcg accgcgtgct ggtctcgacc ggtcgaaagc ccatcaccga cgggctcggt
     1441 ctggccgagc ttggtgtcga gatggacaag cgcggcttcg tcaaagtcaa cgatcattac
     1501 gaaaccaaca tccccggcat ctacgcgatc ggcgacgtga tcggcgggct catgctggcg
     1561 cacaaggccg aggaagaagg catcgccgcc gccgagctga tggcgggcaa ggccggtcac
     1621 gtcaactaca aggtcgttcc cggcgtcgtc tacacgcacc cggaactggc ccaggtcggg
     1681 ctgaccgagg ccgatgcgaa gaaggaaggt tacgaagttc gcgtgggaaa attcccgctg
     1741 aaggcgaacg gccgggcgat ctcgatcgac gacaccgaag gctggatgaa ggtggtcggc
     1801 gacgcgaaaa cggaccggct gctcggcgta cagatcctgt cgccgcacgc cagcgacatc
     1861 atcgccgagg cgtgcgtggc aatggaattc gcggcgagcg tggaagatct ggcgcgaacg
     1921 atgcacgcgc atccgacgct gccggaagcg ctgaaagaag ccgcgctggc ggcggaaaag
     1981 cgcgcgatcc acatctaaag cgcagccgct tgcggcttag catcggttcg ttctcccgcg
     2041 atcgaaaagc tgctaacccg cgagcggcaa ccccatccgg cattccattt gcacgttccc
     2101 aacccacggg cgcagcttta cgcttttgcc agcgtacttc gtcccgccac gacggtctgt
     2161 ccgtcaggag cgttcccatg gccgcgaccg attgccccgc gttgatcgca gtgttcgata
     2221 gccgtgacga agcggagctt gccgtggacg agttgcggga ggccggcttt cgggatgacg
     2281 aaatcggcat cgtcctgcgc ggccgcgacg ccatcgccgg ggggatgatc actgacgccg
     2341 aaggcacgaa ggatgccgtc ggcgccgtcg aaggcgccgc caccggcgcg gtgattggcg
     2401 cgggtttcgg cgcattggtc acgctgttga tccccggcgc cggcccggtc ctggccgcgg
     2461 ggattctcgc caccgcgctg ggttacgccg gcgcgggcgc ggccatcggc ggcatctacg
     2521 gcgcgatgaa aggcctgggc gtttccgaag aagaagcgca gcgctacgaa gaacaattca
     2581 acgccggcaa ggcgatcgtc gccgtgaagg cgggcgcgcg cctgccggac gccatcggca
     2641 tcttccgccg gcatggcgga ttcgatctgc atcaagacgt gaacaacccg gtgccactca
     2701 ccggtccgaa tgaattttga aaccgtgaac gatcgcatcg cgaagaagga gttgatgtat
     2761 gcgaagccat ctgattgaaa acacgatgtc cctgctcggc ggggctggcc tgggggcggc
     2821 gttgatgtac ctgttcgacc ccgaccttgg cgagcagcgc cggcaggacg cccggcaagt
     2881 cgccgggcag gcggtggcca gcaccggtga cgcggtgcaa tcgacggtgc acaccgccac
     2941 cgattcagcg cggtcgttcg cgtcgtgcat cagcgattac gcgcacgacc tggcggatcg
     3001 cgccggcggc caatcgtcga acctcaccga tcgcgccggc gatctggcgt ctggggcgat
     3061 gaagtcggcg cgcaagatgt ggaaccgatc atcgagttcg gccggcgatt acgcgtcgga
     3121 cgccgccgat cgcgcccgcg gcgtgcgcga cgatctgtcc gatcgcgcca gctcgctctg
     3181 gtccagcgcc cgccaatcat cgggaatgga agaatcgcat ccgatcgcca cggcgaccgg
     3241 catcaccgcc gggaccattg gcgtgctggc gctgggcgcc ggcttgatgt acttcatgga
     3301 ccccgagcgc ggccgcggcc gccgggcctg ggcgagcgac aagattttca gcgtcagccg
     3361 ccgcaccggc aaacgcgccc gcagcttagg ccggcacctg ggcaaccgga tgcagggcgt
     3421 tgcccatgag gcgatgaaca gggtccccga aaactggacc ggcggcggca gtggtgaatc
     3481 gtcgcaagcg acgagcggcg agccacagca acagtcgatg ccgtcggcgg ggatgtagag
     3541 atgtgaatcg cgtcccctgg gcatgaacca tttttttagc tgcggcccgc agggccgagt
     3601 gtttcttcgc aactagcgcc aaaacactcg gccctgcggg cggccgctaa actgggtgtc
     3661 tgcaattaga ccgacatcga aagtcttacg cggtctccgc gatcgttcgg cgccggccgc
     3721 gcgcgactcg gggcctgcca aacgcgtccg tcttcgtcat taccttgctc tcgcgatttg
     3781 gatcgatcac cgcggcaatc gcggtgtggt cttcgccatc ggcggtgacc ttctgggcca
     3841 gcgcgtgttc caggcgcttc ctcgcgtagc cgacgtattc ttcgctctgg tcgatgccga
     3901 cgtattgccg gccgaggagg gcggcgctca cgcacgtcgt tccgctgccg ttgaacggat
     3961 cgaggacgac gtcgccgggg ttcgagctgg ctttgatgat gcggttgagg acaccgatcg
     4021 gcatctggca gccgtgccag ccttcgcgtt ctttgaacgt gccgcagacg cggctttcat
     4081 tccacacgtc gtggccgggt tcgaaataac cgttcccccc ggcctcctgc gggcggaggt
     4141 accaggtgtc atccggcagc ttgcctttgg ggttggcgcg atgatcgccg taggtcgtct
     4201 ggcgagcgct ggcgacgcgc accgcgtcgg ggttgaaggt gaaaccttcc ttcgactttg
     4261 tgaaataaaa gatgtgcgtg tggctcttgg cgaacatgaa cttggtttgc tggccaaagg
     4321 tgtaatgcca gatgatccag ttccgcaggt tgaagcccag ttgccgccgg gcgatgacgg
     4381 ccaggtcggc ggcgtattca tcgccgatcg ccagataaaa gctgccggtt ggcttgagcg
     4441 cgcgatgaac ggccgccatc cagtcggtgc tgaacttcag gtaatcctcg gccttcagct
     4501 cgtcgttgta gccgtggtag aggtacccga tgttgaacgg cggatcggcg aagaccaggt
     4561 cgacccagcg ctcggggcct tcgttgagaa ccttgatcga gtcgccctga atgatttggt
     4621 ttgtgttcgg catcccggcc ggattataga gcgttgggcg gatggctcca accgggccgg
     4681 ttttgcgcgt cagtttgtta gaccttcccg ccttcagcgc agacaatccc tgctgaaacg
     4741 cggctttacg c
//
LOCUS       DATIMH010000283         6064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3816237, whole genome shotgun sequence.
ACCESSION   DATIMH010000283 DATIMH010000000
VERSION     DATIMH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3816237"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..2409)
                     /locus_tag="VLI90_04810"
     CDS             complement(271..2409)
                     /locus_tag="VLI90_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HSV13556.1"
                     /translation="MSKCFPRLPALLITLLLAPASGAEATSEWVHPGPDGKLEYKATH
                     AGDRIMDFSYAGYMGGGVALPDVPVKRSVEPSSDKDADDTARIQAAIDDVAKLALAGG
                     FRGAVLLAPGTYNCASPITITASGVVLRGSGAAGPSASTINLTGNPHLAIAVRLGGNG
                     GLRGAPRDATTRETIIAETSIAEAYVPSGAMSLNVRDASSFRVGDAVSIRKPVTPQWV
                     HFMQMDDLVRDGKHQTWIKTGTVLSTERHVAAIAANRITLDVPLSDSIDERFTEPSGG
                     RVARIKPVPRITQAGVENLHIQSPPQAINHTQAHFQALRISGEDCWARDLVIDETMNS
                     VGVNGARLTLQRITVNRKAKHLGASKPAEFAPNGTQVLLDRCNVNADNVWFAATGAQV
                     PGPIVLLNCTFHGNGRAESHQRWSTGMLYDNCRAPEGGIDVRNRGSMGSGHGWPMGWG
                     VLWNCTAKSFLVQNPPGALNWLIGCVGESTQSPRPFGSAPLLPGGVVDSMNAPVTPRS
                     LYLTQLAERLGPQAVRNLGYSSPADVTVADESPRSASDDQTASDELLGQNLALHRPAY
                     TSNVRGNSPEFAGENALDADPNTIWMTDDNAARATLELDMEGGKAINACRIIEARGQR
                     IEEYKIEAQVDSDWRLLAHGTTIEPGHVDRFPKVLAWKVRLTIMNAKQPPAISTFGLY
                     LDKVSPPDQFKAPPPVTEKSEPVKGAPEER"
     gene            2859..3881
                     /gene="aroF"
                     /locus_tag="VLI90_04815"
     CDS             2859..3881
                     /gene="aroF"
                     /locus_tag="VLI90_04815"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSV13557.1"
                     /translation="MVVVMKPGSTQTQVDHVVKLVREMGLKDHVIVGTERTVVAVIGN
                     DRFKDRTVLETVAGVDKVVPILAPYKMASLEVKKERSVIALGGPINAKIGGSKVGVIA
                     GPCSVESKQQTLEIAAAVKEAGAVGLRGGAFKPRTNPYSFQGLAEKGLQILAEAREKT
                     GLAIVTEVMAVDQVKLVADYADVLQVGARNMQNFNLLNAVGEQSKPVLLKRGMSATLE
                     EFLLAAEYIMAKGNAKVMLCERGIRTYEEYVRNTLALAAVPELHRVSHLPVIIDPSQG
                     TGKSHLVDSMCRAAVAAGADGLIIEVHNDPEHAMTDGAQSITPVQFAEMMKSIKRIAA
                     AVDREV"
     gene            complement(4012..4872)
                     /locus_tag="VLI90_04820"
     CDS             complement(4012..4872)
                     /locus_tag="VLI90_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13558.1"
                     /translation="MFGQTFQPHDLLIVLLLVVLEGVLSIDNALVLGLLARRLPAPLQ
                     KKALTYGLIGAFVFRFIAIAVASKLLQWRLVKLLGGGYLVYVALKHLLFEGPPEHEKV
                     VVGADGMPLAVDDRTGRPLTEDELGVEMLRQTHGASVDIEQAVPTTHARAFWAAVASI
                     ELTDIAFAVDSILAAIALVESPDNTPVTGLHPKLWVIITGGMLGVILMRFAAVVFIKL
                     LEKFPRFELSAYLLVLVIGLKLIADWWFNAYTMRLDFHSPTALAFWIFWLLMLICFCV
                     GFMPKRRPLS"
     gene            complement(4985..5389)
                     /locus_tag="VLI90_04825"
     CDS             complement(4985..5389)
                     /locus_tag="VLI90_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13559.1"
                     /translation="MNDATDKTSSGAKPVLDYGHRGLNPHDVRTSRLAIFSLLISLAG
                     TPPFLNLMIAWSLRRWLLRVGVQRYGTERIVFGCVMTPTFVALILATCAAWRIGCNDR
                     LRGEALAMIVMSICVFWLFGGACFFMFGLYEG"
     gene            complement(5386..>6064)
                     /gene="upp"
                     /locus_tag="VLI90_04830"
     CDS             complement(5386..>6064)
                     /gene="upp"
                     /locus_tag="VLI90_04830"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HSV13560.1"
                     /translation="RRDRGGTSIDWGPVARMSLSNVFLIEHPVIQTKLTELRDFTTDH
                     RKFRALLNEIASLMVYEVTRDWPTRPKPVQTPLERMTGQVLARRVTLVPILRAGLGMA
                     DGVLHLLPDARLGHLGVYRDEKTLEPVSYYQKLPPDIADTEVLLIDPMLATGGSGSAA
                     VAFLKKAGVKTMKFVCLVAAPEGIQAIHRQHPEIPIYAAAIDRQLNEKGYILPGLGDA
                     GDRIFGT"
BASE COUNT         1123 a         1805 c         2023 g         1113 t
ORIGIN      
        1 actatatctg atccgcctgg cggcgttcgt gctgatcatc atcgcgatcg cagataaaaa
       61 tcgcgccgcg cggcggaatg gttcgcgaaa ataatgcctc tacggcgcgg cggacgccgc
      121 ggagctttag cccctgtagc tgcgacgcct gcgtcgcctt ggcgttcctt gccaaggagg
      181 cgacgcaggc gtcgcagcta caaagtcacg cagttcgctg tgcatcccct tggcgtttcc
      241 gcccgcgtgg ctgcgatatc cgatgtcggc ttaccgttcc tccggcgcgc ccttcaccgg
      301 ctccgatttc tctgtcacgg gcggcggcgc tttaaactga tccggcggtg agaccttgtc
      361 gagatataag ccgaacgtgc tgattgccgg cggttgcttg gcgttcatga tcgtcagccg
      421 aactttccac gccaggactt tggggaagcg atcgacgtgg ccgggttcga tcgtggtgcc
      481 gtgcgcgagc agccgccagt cgctgtccac ctgagcttcg attttatatt cctcgatccg
      541 ctgccccctg gcttcgatga tccggcaagc gttgatggcc ttgccgcctt ccatgtccaa
      601 ctccagcgtt gcgcgggcag cgttgtcgtc ggtcatccag atcgtattcg ggtcggcatc
      661 cagcgcgttc tcgccggcga attcggggct gttgccccgg acgttgctcg tgtatgcggg
      721 gcgatgcagg gcgaggtttt ggccgagcag ctcgtccgat gcagtttgat cgtccgacgc
      781 acttcgcggc gattcatcgg ccaccgtcac atccgccggc gatgagtaac cgagattccg
      841 caccgcctgc gggccgaggc gctcggccag ctgcgtcaga taaagactgc gtggcgtgac
      901 cggcgcgttc atcgaatcga ccacaccgcc cggcagcagc ggagcggagc cgaacggccg
      961 cggggattgg gtcgattcac cgacgcagcc gatcagccag ttcagcgcgc ccggcgggtt
     1021 ctgcacgagg aaacttttgg ccgtgcagtt ccacaaaacg ccccagccca tcggccagcc
     1081 gtgacccgag cccatcgacc cgcggttgcg cacgtcgatg ccgccttcgg gtgctcggca
     1141 gttgtcgtat agcatgccgg tggaccagcg ctggtgcgac tcggctcgac cgttgccgtg
     1201 gaacgtgcag ttcagcagca cgatcggtcc cggcacctgg gcgccggtgg cggcgaacca
     1261 gacgttgtcg gcgttcacgt tgcaccggtc cagcagcacc tgcgtcccgt tgggcgcgaa
     1321 ctccgccggc ttggacgcgc ccaggtgttt tgcctttcga ttgacggtga tgcgctgcag
     1381 cgtgaggcgc gcgccgttca cgccgacgct gttcatcgtc tcatcgatta caaggtcgcg
     1441 cgcccagcaa tcttcgccgc tgatgcgcag cgcctggaaa tgcgcttgcg tgtggttgat
     1501 cgcctgcggc ggcgactgga tgtgcagatt ctccacgccc gcctgcgtga tgcgcggcac
     1561 cggcttgatc cgcgcgaccc ggccgccgct cggttccgtg aaccgctcgt cgatcgagtc
     1621 ggacagcggc acgtcgagcg tgatgcgatt ggcggcgatg gcggcgacgt gccgctcggt
     1681 ggagaggacc gtgccggtct tgatccacgt ctgatgcttc ccgtcgcgga cgagatcgtc
     1741 catctgcatg aagtggaccc actgcggcgt gaccggcttg cggatggaaa ccgcgtcgcc
     1801 aacgcggaac gagctggcgt cgcggacgtt cagcgacatc gcgcccgacg gcacgtacgc
     1861 ctcggcgata gaagtctcgg cgatgatggt ttctcgtgtt gtcgcatcgc gcggcgcgcc
     1921 acgcaggcca ccattgccgc cgaggcgcac ggcgatcgcc aggtgcgggt tgccggtcag
     1981 gttgatcgtg ctggcggagg ggccggccgc gccgctgccg cgcaggacga cgccgctggc
     2041 ggtgatcgtg atggggctgg cgcagttgta cgtccccggc gcgagcagca ccgcgccgcg
     2101 gaagccaccg gcgagcgcca gcttggcgac gtcgtcaatg gcggcctgaa tccgcgcggt
     2161 gtcgtcggca tctttgtcgc tcgaaggctc gacgctgcgc ttcaccggca cgtccggcag
     2221 cgcgaccccg ccgcccatgt agccggcgta ggagaaatcc atgatgcgat cgccagcgtg
     2281 ggtggctttg tattcgagct tgccatcggg tccggggtgg acccattcgc tggtggcttc
     2341 agcgccggac gcgggggcaa gcaacaacgt gatcaacaaa gcgggcaggc gcggaaagca
     2401 cttggacatg atcatccata ccgccgtcaa tcgattgcgt ttgcgagtcc cgcggcagag
     2461 cggtgggctt tttgggtggc tggatttcac tttgacaagc ggcatcggcg tgatattgtg
     2521 gttttcaacg tgagccagtt cagcgcgagc ttcgggtctt ggtttagcgg ttactttacc
     2581 gccgcccgat cctgcgcgtt ctgagctgac accgatacac cggtagaatt tcaaaagcct
     2641 caggacgcgg tcctggggct ttttgcgttt gacgcgaaga ggattaacca cagatgtaca
     2701 cagatgaacg cagatgaggg aaaagtagca cgggcgtccc gcacgtgtct aatggcgcca
     2761 gacaccggcg agacgcccgt gctacctttc ttatcggtgt gcatccgtgt gcatctgtgg
     2821 ttccattctc ctgattttca aacattggag gccccgcgat ggtcgtcgtc atgaagccgg
     2881 gatcgactca gactcaggtc gatcatgtcg tcaaactggt gagagaaatg ggactcaaag
     2941 atcacgttat cgtcggcacc gaacgcaccg tggtggccgt catcggcaac gaccgcttca
     3001 aggaccgcac ggtgctcgaa accgtcgccg gcgtcgataa ggtcgtgccg atcctcgcgc
     3061 cgtacaagat ggcttcgctg gaagtgaaga aggagcggtc ggtcatcgcg ctgggtgggc
     3121 cgatcaacgc gaaaatcggc ggcagcaaag tgggcgtgat cgccgggccg tgcagcgtgg
     3181 agagcaagca gcaaacgctg gagatcgctg ctgcggtgaa ggaagccgga gccgttggcc
     3241 tgcgcggtgg ggcgttcaag ccgcgcacga atccctattc attccagggt ttggcggaga
     3301 agggtctgca gatcctggcc gaggcgcgcg agaagacggg gctggcgatc gtcaccgagg
     3361 tgatggccgt cgatcaggtc aaattagttg cggattatgc ggatgtatta caagtgggcg
     3421 cccgcaacat gcagaacttc aacctgctga acgcggtggg cgagcagagc aagccggtat
     3481 tgctgaagcg cggcatgagc gccacgctgg aagaattcct gctggccgcc gaatacatca
     3541 tggccaaggg caacgcgaag gtgatgctgt gcgagcgcgg catccgcacg tacgaagaat
     3601 acgtccgcaa cacgctggcg ctggcggcgg tgccggagct gcaccgcgtg agccatctgc
     3661 cggtgataat tgaccccagc cagggcacgg gcaagagtca cctggtggac agcatgtgcc
     3721 gagccgccgt cgcagcgggg gcggatgggt tgattatcga agtgcacaat gatccggagc
     3781 acgcgatgac ggacggggca cagagcatca cgccggtgca gttcgcggag atgatgaagt
     3841 cgatcaagcg gattgccgcg gcggtggatc gggaagtcta aagtttatcc tgagcgcagc
     3901 acgtactcgt gcggagtcga aggatctggc agtggagtcg gtgaacctct aatcgcgaga
     3961 acagcaccga cgtccagcga tatccttcga ctccggcgcg tccgcctgcg ctcacgacaa
     4021 ggggcggcgc ttgggcataa acccgacgca gaagcagatc agcatcagca gccagaagat
     4081 ccagaacgcc agcgcggtgg ggctgtggaa atccagccgc atggtgtacg cgttgaacca
     4141 ccagtcggcg atgagtttga ggccgatgac cagcacaagt aagtaagcgg acaactcaaa
     4201 ccgtgggaac ttctcgagca gcttgatgaa gacgacggcg gcaaagcgca tcaggatcac
     4261 gccgagcatg ccgccggtga tgatgaccca gagcttgggg tgcaacccgg tgacgggcgt
     4321 gttgtcgggc gattcgacca gcgcgatggc ggcgaggatc gagtccaccg cgaaggcgat
     4381 gtcggtcagc tcgatcgagg cgacggcggc ccagaacgcg cgggcgtggg tggtggggac
     4441 ggcctgctcg atatcgacac tggcgccgtg cgtttgccgc agcatctcca cgcccagttc
     4501 gtcctcggtc agcgggcggc cagtgcgatc gtccacggcg agcggcattc catcggcgcc
     4561 gacgacgacc ttctcatgtt ccggcggccc ttcgaacagc aggtgcttga gcgcgacgta
     4621 gacgagatac ccgccgccca gcagcttcac gagccgccat tgcagcagct tggacgcaac
     4681 ggcgatggcg atgaagcgga agacgaacgc gccgatcagg ccataggtga gggctttttt
     4741 ctgaagcggc gcgggcaatc ggcgcgcgag caggccgagg acgagggcgt tgtcaatcga
     4801 gagaacgcct tcgaggacga cgaggagcag gacgatcagc agatcgtggg gttggaaggt
     4861 ctggccgaac attcaatgca gcgacagcac gctacgcggg gggttgtaca tgttttggcg
     4921 gtcaaactct attagaacga cggcggcttt tttcacaccg cgcgtttcag tccggcttgt
     4981 gttttcaccc ctcgtaaagt ccgaacatga aaaagcacgc gccgccgaaa agccaaaaga
     5041 cgcagatcga catcacgatc atcgccagcg cctcgccgcg caagcggtcg ttacaaccga
     5101 tgcgccacgc cgcgcacgtc gcaaggatca acgcgacaaa tgtgggcgtc ataacgcagc
     5161 cgaagacgat gcgctccgtc ccgtaccgct gcacgccaac gcgcaacaac cagcggcgca
     5221 aactccaagc aatcatcagg ttcaggaagg ggggagtgcc tgcaaggctt atcaacagcg
     5281 agaagatcgc caagcggctc gttcgcacgt cgtgcggatt cagaccgcga tggccgtaat
     5341 cgagtacggg cttggcgccc gaggaggttt tatccgtcgc gtcgttcacg ttccaaatat
     5401 acggtctccg gcgtcgccga gtcccggcag aatatatccc ttctcattca gctgccggtc
     5461 gatcgcggcg gcataaatcg ggatctccgg gtgctggcgg tggatcgctt ggatgccttc
     5521 gggggcggcg acgaggcaga cgaacttcat cgtcttcacg ccggcttttt ttaggaacgc
     5581 aacggcggcg gagccgctgc caccggtggc gagcatcggg tcgatcagca gcacttcggt
     5641 gtcggcaatg tcgggcggca gcttctgata atagctgaca ggttcgagcg ttttttcatc
     5701 gcgatagacg ccgagatgac ccagccgggc gtcgggcagc aggtgcagca caccatcggc
     5761 catgccgagg ccggcgcgaa ggatcggcac cagcgtcacg cggcgggcca ggacctggcc
     5821 ggtcattcgc tccagtggcg tctgcacggg cttgggccgc gtgggccagt cgcgcgtgac
     5881 ttcgtagacc atcagcgatg cgatttcgtt caacagcgcg cggaattttc ggtggtcggt
     5941 ggtgaaatcg cgaagctccg tgagcttcgt ctggatgacg gggtgttcga tcaggaagac
     6001 gtttgacaag gacattcgcg ctaccgggcc ccagtcgatc gacgtgccgc cacggtcacg
     6061 tcgt
//
LOCUS       DATIMH010000284         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4104237, whole genome shotgun sequence.
ACCESSION   DATIMH010000284 DATIMH010000000
VERSION     DATIMH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4104237"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1011
                     /locus_tag="VLI90_04835"
     CDS             271..1011
                     /locus_tag="VLI90_04835"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HSV13561.1"
                     /translation="MAKLSVNVNKVATLRNTRDLGIPSVIRASQICLNAGAHGITVHP
                     RPDQRHIRPSDVHEIAQLLKSYPNSEFNIEGNPFEHYMHYAKDERPTQCTLVPDTRDV
                     PTSNTGWDLTAANIDRLRPVIAELKSYGCRVSLFLNPDLSQVDSAAAIGADRIELYTE
                     PYAAAFARGAAQAADAYVAAANHAKARGLQVNAGHDLNLANLPPLVEKIPFLAEVSIG
                     HALIADALELGLAETVRRYLRAAAGPGH"
     gene            1105..1977
                     /gene="dapA"
                     /locus_tag="VLI90_04840"
     CDS             1105..1977
                     /gene="dapA"
                     /locus_tag="VLI90_04840"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HSV13562.1"
                     /translation="MFSGAFTAMVTPFREGRLDEARLREQVEHQIRGGIDGLVPVGTT
                     GESPTLNFDEHVKVIELTVKFAAGRVPVVAGTGANATSEAVELHAAAKKVGATACLSV
                     NPYYNKPTQEGLYRHFMTLADRVDLPIVLYNIPGRTGITMTPQTVARLNEHKNIIAIK
                     EATGSLDMASEILSLCDITVMSGDDSLTLPIMSVGGTGVISVASNLIPTKIKSLTQLA
                     RTGNFAQAKQIHLELFGFIKTLFLDGNPAGIKHAMKVAGLDSGEMRLPLWEASEATKK
                     VIEQQVARLGVTAR"
     gene            2077..2616
                     /locus_tag="VLI90_04845"
     CDS             2077..2616
                     /locus_tag="VLI90_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13563.1"
                     /translation="MAVYFITLHAYRSWRPDHKRGYTRRGKGVLPPDPQTAANYDAAA
                     HQEPVEFTKEMQQVMVAGAFDVCARRGWRLHGAGFELTHGHFLVSWRGFISWLDVRAK
                     LKNLLSLFLGRLTGKQNQNWFVDGGSRKWVKTRGHFNFLMKTYLPKHHGEKWYEGDPL
                     PVIPEHILNPPRNDGRDTK"
BASE COUNT          546 a          835 c          832 g          459 t
ORIGIN      
        1 agcagatcgg cagcggggcg atgctgagcc gggctgtggg tggaattgca aaaaggaaat
       61 tgctcttcgc aatgccggga tccaccgccg cggtggagct ggcgatgacg aagctgattc
      121 tgccggaatt gaagcatctg ctgcatgaat tgcgcaaatg atccgacggt ttgcttctgt
      181 aatagcccag gccggaaggg cggggatcaa atccgccaat tgtcgaagtc tgatccccgg
      241 ccttccggcc taggctatta ttcttcgcct atggccaagc tcagcgtcaa cgtcaataag
      301 gtcgccaccc ttcgcaacac ccgcgacctc ggcatcccca gcgtcatccg cgcttcgcag
      361 atctgcctca acgccggggc gcacggcatc accgtgcacc cgcggccgga ccagcggcac
      421 atccggccca gcgacgtgca tgagatcgcg cagctgctga agtcttaccc aaattccgag
      481 ttcaatatcg aaggcaaccc gttcgagcac tacatgcact acgcgaaaga cgagcggccc
      541 acgcagtgca cgctggtgcc ggacacgcgg gatgtgccga cgagcaacac cggctgggac
      601 ctgacggcgg cgaacatcga ccgcctgcgg ccggtgatcg ccgagctcaa aagctatggc
      661 tgccgcgtca gtctgtttct caatccggat ctgtcgcagg tcgatagcgc cgcggcgatc
      721 ggcgccgatc gcatcgagct gtacaccgag ccgtatgctg cggcgtttgc gcgcggcgct
      781 gcgcaggcgg cggatgcgta cgtcgcggcg gcgaatcacg cgaaagcgcg aggcctgcaa
      841 gtgaacgcgg ggcacgatct gaacctggcg aacctgccac cgctggtcga gaagattccg
      901 ttcctcgcgg aagtgtcgat cggccacgcg ctcatcgcgg atgcgctcga acttgggctc
      961 gccgaaaccg tgcggcgtta tctgcgcgcg gcggcaggtc ccggccactg aagtggcacg
     1021 ggcctcggcc cgcgcacaat ccgcgcttgc ccgttgcttg aatccaccta gaatcacccg
     1081 gctttaccga cacggagttt gcccatgttc agcggcgcgt tcacagcaat ggtcaccccc
     1141 ttccgcgaag gccggctcga tgaggcccgc ctgcgcgagc aggtcgaaca ccagatccgc
     1201 ggcgggatcg acgggctcgt ccccgtcggc accaccggcg aatcgcccac gctcaatttc
     1261 gacgagcacg tcaaggtgat cgagctgacc gtgaagttcg ccgcgggccg ggtgccggtc
     1321 gtcgccggca ccggcgcgaa cgcgaccagc gaagcggtcg agctgcacgc cgccgcgaag
     1381 aaggtcggcg ccaccgcgtg cctcagcgtc aatccttatt acaacaagcc gacgcaggaa
     1441 gggctgtatc gtcatttcat gacgctggcc gatcgggtcg atttgccgat cgtgctttac
     1501 aacattcctg gtcgcaccgg catcacgatg acgccgcaga ccgtggcgcg cctgaacgaa
     1561 cacaagaaca tcatcgccat taaagaagca accggctcgc tcgacatggc cagcgagata
     1621 ctcagcctct gcgacatcac ggtgatgagc ggcgacgatt cgctgacgct gccgatcatg
     1681 agcgtcggcg gcactggcgt gatcagcgtc gcgtcgaacc tgatcccgac gaagatcaaa
     1741 tcgctgacgc aactggcccg caccggtaac ttcgcccagg cgaagcagat tcacctggag
     1801 ctgttcgggt ttatcaagac gctgttcctc gacggcaacc ccgccggcat caagcacgcg
     1861 atgaaagtcg cgggactcga cagcggcgag atgcgcctgc cgctgtggga agcaagcgag
     1921 gcgacgaaga aggtgatcga acagcaggtg gcgcggctgg gtgttacagc gcgctaattt
     1981 tgatagccgg gggcggaagt cccggtcttc taacgctcaa acggaaaaga ccggagcttc
     2041 cgcttccggc tatcaaagct cgaagaggcg cgcgccatgg ccgtctactt catcacgctg
     2101 cacgcgtatc gctcctggcg tcccgatcac aagcgcggct acacgcgacg aggcaaaggc
     2161 gttttaccgc cggacccgca aacggcagca aattacgacg cggcggcgca tcaggaacca
     2221 gtggagttca cgaaggagat gcagcaagtc atggtcgccg gcgcgttcga cgtctgcgct
     2281 cggcgcggat ggcggttgca cggcgccggc ttcgagctca cgcacggcca ttttctcgtc
     2341 agctggcgcg gcttcatttc ctggctcgat gttcgcgcga agttgaagaa cctgctcagc
     2401 ctttttctcg gacgactgac ggggaaacag aaccagaatt ggtttgtcga cggcggcagc
     2461 cgcaaatggg tgaagacgcg cggtcatttt aactttctga tgaagacgta cctgccgaag
     2521 catcacggcg agaagtggta cgaaggcgat ccgctgccgg tgattccaga gcacattttg
     2581 aatccgccgc ggaacgacgg acgcgatacg aagtgatagc cggaagcgga agctccggtt
     2641 tttccgttcg agcattaaaa gacccgggct tc
//
LOCUS       DATIMH010000285         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_352269, whole genome shotgun sequence.
ACCESSION   DATIMH010000285 DATIMH010000000
VERSION     DATIMH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352269"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VLI90_04850"
     CDS             <1..495
                     /locus_tag="VLI90_04850"
                     /inference="COORDINATES: protein motif:HMM:NF037386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13564.1"
                     /translation="ITMRSTFIVGFPGETEEHVAYLEEWIGRAQLDRVGFFEYSQEEG
                     TPSADLPGQVPAQEKRRRLVRLREAQRLASSKARGRRAGEQVRVLVEERRSLRKTDPL
                     RAGLGSSQAWFGRSMGEAPGVDGGVYFTGEAEPGQFVEVTLEGHGPFDFFGRITSKET
                     ATIG"
     gene            488..1843
                     /locus_tag="VLI90_04855"
     CDS             488..1843
                     /locus_tag="VLI90_04855"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HSV13565.1"
                     /translation="MDNRVKRIALTVAALLVLGFVAVVVGFSVAFHRSPLSVLAGQLT
                     PTPQELFHKDHILVLAEGLDYDYTDTDQEFSKQSRSDVIKAINLDFRTKNVYVVSIPR
                     DMDAVLPSGRESKINEAQAEGGVREAQNVIASWLGIPGFDRYILLRINTTKDLINAIG
                     GIDINPKNSDALKGTGPNGPIDYDDSWGHLHIHFKPGMQHMNGEQAVSYARFRHDWCS
                     DECRILRQQQVMSAALNKLRSDKFNTLVHLGDLIGVFNRDVQTNLTRDEQLSLAQMFS
                     TMPKDGLHQKPIDYVDTKMLSDGGEVIIPNQAQKQALVQNMLLDPPVPTPTPAAGAVA
                     AINPFSVRVDVENGTGIAGMAKRAAAVLREKGFTIGTVSNAATSDVATTELHEHSKIT
                     FAGLRVREALGKAASSAPVIADSEPATPEASGDAPQSDVTLVVGQDLVPSLTQQASTQ
                     P"
     gene            1840..2661
                     /locus_tag="VLI90_04860"
     CDS             1840..2661
                     /locus_tag="VLI90_04860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSV13566.1"
                     /translation="MNPRAWVAAAIVASAVLAYAGYRVILHASHIQPAIVTPKMDAQQ
                     AISSSFAGHVKRLLNERVTYANREGRPKLIALTFDDGPYPIFTPLLIDELRKLDVPAT
                     FFLIGRDAQQWPELAQRIERSGNEIADHTYTHPNLDQESPPQVRREILEGRDVLYGMV
                     HDPALLDEFRPPHGRYTLQTIQVAQSLGYKTILWTDDGGDWRTVTARSLADHLEQHAT
                     APEIVLLHSGKLATIEMLPEVVARFRAAGYRFVTVGQMLRQVPAADLNHPAKRAV"
BASE COUNT          575 a          878 c          874 g          494 t
ORIGIN      
        1 atcacgatgc gatcgacgtt catcgtcggc ttcccaggcg aaactgagga acacgtcgct
       61 tatttagagg agtggatcgg gcgtgcgcag ctcgaccgtg tcggcttctt cgagtacagc
      121 caagaagaag gcacgccgag cgcggacctg cccgggcaag tccccgctca ggagaaacgg
      181 cgcaggctcg tccggctccg cgaagcccaa cgtcttgcct catcgaaggc ccgcgggcgg
      241 cgcgccggcg agcaggtgcg cgttctcgtg gaagaacggc gctcgctgcg caagaccgac
      301 cccctgcgtg ccggattagg cagcagccag gcgtggtttg ggcgctccat gggcgaagca
      361 ccgggtgtag acgggggcgt ctacttcacg ggcgaggccg agccggggca gttcgtcgag
      421 gtcacgctcg aaggacacgg cccgttcgac ttcttcgggc gtatcacttc aaaggaaaca
      481 gcgacgattg gataaccgcg ttaaacgcat agcccttacc gtagccgcgc ttttggtgct
      541 gggctttgtc gccgtcgttg tcggattttc cgtcgcgttc caccgctcgc cgctctctgt
      601 gctggccggc cagctgaccc ccacgccgca agaactcttc cataaagatc atatcctggt
      661 gcttgccgaa ggcttggatt acgactacac cgacacggac caagagtttt ccaagcagtc
      721 gcgcagcgat gtgatcaagg cgatcaatct ggatttccgc accaaaaacg tgtacgtcgt
      781 ctcgattccg cgagacatgg atgcggtatt gcctagcgga cgcgaatcga agatcaatga
      841 agcgcaagcc gagggcggtg tgcgcgaagc gcaaaacgtc attgcgagct ggctgggcat
      901 tcccggcttc gaccgttata ttctgctgcg catcaacacg acgaaagatc tgatcaacgc
      961 gatcggcggc atcgacatca acccgaagaa ttcggatgcg ctcaaaggca cggggcccaa
     1021 cggccccatc gattacgacg acagctgggg acatctgcac atccatttca aacccggcat
     1081 gcagcacatg aatggcgagc aagcggtgag ctacgcccgt ttccggcacg attggtgcag
     1141 cgacgaatgc cgcattctgc gccagcagca agtcatgtcc gccgcgctga acaaactacg
     1201 cagcgataaa ttcaacacgc tcgtgcatct gggcgatctt atcggcgttt tcaatcgcga
     1261 cgtgcagacc aatctcaccc gcgacgagca gctcagccta gcgcagatgt tctctacgat
     1321 gcccaaagac gggttgcacc aaaaaccaat cgactacgtc gatacgaaaa tgctctccga
     1381 cggcggcgaa gtcatcatcc ccaatcaggc gcagaaacaa gcgctcgttc aaaacatgct
     1441 gttggatccg ccggtcccca cgcccacccc ggcggccggc gcggttgcgg ccatcaatcc
     1501 gttcagcgtg cgcgtcgacg tggaaaacgg caccggcatt gcgggaatgg caaaacgcgc
     1561 cgcggcggtg ctgcgcgaga agggctttac gatcgggacc gtttcgaacg cggcgaccag
     1621 cgacgttgcg acgaccgaac tgcacgaaca ctctaagatc acgttcgccg gtttgcgcgt
     1681 gcgcgaggcg ctcggaaaag ccgcgagcag cgcgccggtg atcgcagatt ccgaaccggc
     1741 tacgcctgaa gcctcgggcg atgcgccgca gagtgacgtg acgctggtgg tgggacaaga
     1801 tctcgtcccc tcgttgacgc agcaggcctc aacgcagccg tgaacccgcg cgcctgggtc
     1861 gccgccgcga tcgtagcgag cgcggtgctg gcgtatgccg gttaccgcgt gatcttgcac
     1921 gcatcgcaca tacaaccggc gatcgttacg ccgaagatgg acgcgcaaca ggcgatctcg
     1981 tcgtcattcg cggggcacgt aaagcggttg ctcaacgaac gcgtcacgta cgcgaaccgc
     2041 gaagggcgtc cgaaattgat cgcgctcacg ttcgacgatg ggccgtatcc gatttttacg
     2101 ccgctgctca tcgacgaact gcgcaaactc gacgttccgg cgacgttctt ccttatcggt
     2161 cgcgacgcgc agcagtggcc cgagctggcg cagcgcatcg agcgctcggg caacgagatc
     2221 gccgatcaca cctacacgca cccgaatctg gatcaggagt cgcccccgca ggtccgccgg
     2281 gaaatcctgg aagggcgcga cgtgctctac gggatggttc acgatccggc actgctggat
     2341 gagttccggc cgccgcacgg ccgctacacg ctgcagacga ttcaggtggc ccagtcactg
     2401 gggtacaaga cgatattgtg gacggatgac ggcggggatt ggcggacggt gaccgcgcgc
     2461 tccctggcgg accacttgga gcagcatgcg acggcgccgg agatcgtctt gctgcacagc
     2521 gggaaactgg cgaccatcga aatgcttccg gaggtggtcg cgcgtttccg cgccgccggc
     2581 taccgcttcg tgaccgtggg gcagatgctc cgccaggtgc cggccgccga tttgaaccat
     2641 ccggcgaagc gcgccgttta aggagaacct gtgcctcgac agatcatcga tacggaatcg
     2701 agccgcccgc aatacattcg ccggctggtc atcaccacgg cggtcattgt aatcgtggtc
     2761 gcggtgctcc taatccttgc cttcgcggcg tggggacgct atgggacctc caaagggaat
     2821 g
//
LOCUS       DATIMH010000286         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4104518, whole genome shotgun sequence.
ACCESSION   DATIMH010000286 DATIMH010000000
VERSION     DATIMH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4104518"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VLI90_04865"
     CDS             complement(<1..362)
                     /locus_tag="VLI90_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSV13567.1"
                     /translation="MPRPNIVVILADDLGFSDIGCYGGEIHTPNIDALASDGVRVAQF
                     YNMARCCPTRAALLTGLYPHQAGVGAMNQDLGKPAYQGELNARCATIAEVLRTAGYHT
                     GMVGKWHLSHLTIASAPR"
     gene            complement(440..>2510)
                     /locus_tag="VLI90_04870"
     CDS             complement(440..>2510)
                     /locus_tag="VLI90_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HSV13568.1"
                     /translation="LRDYYDATADPRVLPFLRNYYHYMLRSLPGRPLRDWAKSRAGDD
                     MDTAIWLYDRTGEPFLLELVDLLRRQAYDWPTIMHQNSFESFDKDFQPKHNVNIPQAM
                     KMPAVSWQRTGDERERTAIDAGEANLMRDHGLSIGMQSGTEFLAGRSPGQGVEFCAIV
                     EQMLSDETIVRIFGDGTYADHLEQIAYNALPAAWNRDLTALRYYTLPNHVIAKRGPQG
                     FGQNYDNGVVYGPRSGYPCCCFNVHQGWPKFVQNSWAATSDNGLAAIAYAPTTVTASV
                     GDGSGATATIVEETNYPFDDEIRFKITMSAPATFPIALRTPGWCDRASITVNGRSVEA
                     AKPGDFQKITRQWNSGDEIVVKLPMTVRTVSGVHGSVSVHRGPLVYSLQIEHDKHIVD
                     QPAPGFNEFEETPTGQWNYALDPGRDIELLRDEKPLARANPFTPEATPIKLTASARQL
                     PAWGLAWNGVVALDPPISPVQSNERETRVTLVPFGAEDLRLTDFPILGEPAGAPPNPL
                     TFPFDQNDTTGWTTIGGGWWVHDGRLRTTPNGGAPGYKALLENLTCADVRIEADVTPP
                     PTGDAGVIFRVSKPSIGADAYQGYYAGISASGNQVILGRADGLTWTPLKIVPHVMSAE
                     RSTKLSVTAVGNGIEVRVNDETSPAIAIADDRWTTGQIGVRMFTSDNDHAISAFGNVR
                     VIPGSQR"
BASE COUNT          410 a          768 c          841 g          491 t
ORIGIN      
        1 tcgcgcggag cgctggcgat cgtcaggtgc gacagatgcc atttgcccac catgcccgtg
       61 tgataccccg cggtccgcag cacctcggcg atcgtcgcgc agcgggcgtt caactcgcct
      121 tgatatgccg gcttgcccag atcttggttc atcgcgccga cgcccgcctg gtgtgggtaa
      181 agccccgtca gcaacgccgc ccgcgtgggg cagcagcggg ccatgttgta gaactgcgca
      241 acccgcacgc cgtcggacgc aagcgcgtcg atgttcggcg tgtggatctc accgccgtaa
      301 caaccgatat ctgaaaaccc caggtcatcg gcgagaatga caacaatgtt ggggcgcggc
      361 atcggctgag cgcaaacggt ctgggtcacg acagcacagc aaagaacgat cgccggacca
      421 atgcagccga gccggtgcgt catcgctggc tcccgggaat gacgcggacg ttgccgaagg
      481 ccgatatcgc gtgatcatta tcgctggtaa acatgcgcac cccaatttgg cccgttgtcc
      541 atcggtcgtc ggcgatcgca atggcaggcg acgtttcgtc gttcacgcgc acttcaatgc
      601 cgttaccgac ggcagtgacg cttaacttgg tacttcgctc agcggacatc acgtgaggga
      661 cgatcttcag cggcgtccag gtaagtccgt cggcgcggcc gaggatgact tggtttccac
      721 tagcgctaat gcccgcgtaa tagccttgat acgcatcggc gccgatcgac ggtttgctga
      781 cgcggaagat gacaccggcg tcgccggtgg gtggcggtgt gacgtccgcc tcgatgcgga
      841 cgtcggcgca ggtgaggttt tcgagcagcg ccttatagcc gggcgcgccg ccattggggg
      901 tggttcggag tcggccgtcg tgaacccacc aaccgccgcc gatcgtggtc cagccggtgg
      961 tgtcgttttg gtcaaagggg aatgtgagcg ggttcggcgg cgcgccggcc ggttctccga
     1021 gaattggaaa atccgtcagc cgtagatcct ccgcgccgaa cggcacgagc gtgacccgcg
     1081 tttcgcgctc gttcgattgc accgggctga tcggcggatc gagcgcgacg acgccgttcc
     1141 acgccaagcc ccaagcgggt aactgccgcg cgctggcggt cagcttgatc ggcgtcgctt
     1201 cgggcgtgaa cggattggct cgcgcaagcg gcttttcgtc tcgtagtagt tctatgtccc
     1261 tgccgggatc gagcgcatag ttccactggc ccgttggcgt ttcttcaaat tcattgaagc
     1321 ccggcgcggg ctggtcgacg atgtgcttgt catgctcgat ctgcagtgaa tacacgagcg
     1381 gcccgcggtg aacgctgacg ctgccgtgta cgccgctcac cgtgcggacg gtcatcggta
     1441 acttgacgac gatttcatcg ccgctgttcc actggcgggt gatcttctga aaatcgccgg
     1501 gcttggccgc ttcgacggac cggccgttga cggtgatgct cgctcgatcg caccagcccg
     1561 gcgtgcgcag cgcgatgggg aacgtggccg gcgcgctcat cgtgatcttg aagcggatct
     1621 cgtcgtcgaa cggatagttt gtctcttcga cgatcgtcgc ggtcgcgccg ctgccgtcgc
     1681 cgacactggc ggtgacggtc gttggcgcgt acgcgatcgc cgccaggccg ttgtcgctcg
     1741 tggcggccca actgttctgc acgaattttg gccagccttg gtggacgttg aagcagcaac
     1801 aggggtaacc cgagcgcggg ccgtagacga cgccgttgtc gtaattctgg ccgaagccct
     1861 gcgggccgcg cttggcgatg acgtgattgg gcagcgtgta ataccgcagc gccgtcaggt
     1921 cgcggttcca cgcggcgggc agggcgttgt aggcgatctg ctccagatgg tccgcatatg
     1981 tgccgtcgcc gaagatgcgg acgatcgttt cgtcgctgag catctgctcg acgatcgcgc
     2041 agaactccac gccttggcca ggacttcgcc cggccaggaa ctcggtgccg ctttgcatgc
     2101 cgatcgacag gccgtggtcg cgcatgaggt tcgcctcgcc cgcgtcgatc gcggtgcgct
     2161 cgcgctcgtc accggtgcgc tgccacgaca ccgcgggcat cttcatcgcc tgcgggatgt
     2221 tcacgttgtg cttgggctga aagtccttgt cgaaggactc gaagctgttc tggtgcatga
     2281 tggtcggcca gtcgtacgcc tggcggcgca gcagatcgac cagctcgagc aggaacggtt
     2341 cgcccgtgcg gtcgtagagc cagatggcgg tgtccatgtc atcaccggcc cggctcttgg
     2401 cccagtcgcg cagcggacgg ccgggcaggc tacgtagcat gtaatgatag taattgcgca
     2461 gaaacggcaa cacgcgcggg tcggcggtgg cgtcgtagta gtcacggaga
//
LOCUS       DATIMH010000287         4343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4685994, whole genome shotgun sequence.
ACCESSION   DATIMH010000287 DATIMH010000000
VERSION     DATIMH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4685994"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..2090
                     /locus_tag="VLI90_04875"
     CDS             330..2090
                     /locus_tag="VLI90_04875"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HSV13569.1"
                     /translation="MRIYIALAAGLVCVRTATATDILVENFDEPGGFFAGSGLHTPSD
                     PPPDWQVVNNSQPQAANSNSWFDAPNANYNYFQAQSGANFAGVDAASGSNVATLNNWL
                     ITPQISFNAGDQLSFYSRSKGSYPDRLQVRLSFNGASASAGTTSTSTGDFSTTLLDIN
                     PTYLASNNGGYPATWTPYTLSFPAAGSGRLAFRYFVEDGGPGGDNSDFLGVDTLAVVT
                     GAPANQWAATTGGSWNNAGNWASGIPNAAAAKVGLGSGATSSAVVTLDGNVTAGQITF
                     NNANGYSLQPGAGGTLKLDDTGDAGGVHPSVSVVSGSHLISAPITLTSGLTIATSAGG
                     ALNITSDISGSGDIAVAGPGTVHLSGALPTTAAINANGAVVLAAQTSSGAAARTIAAL
                     NIASGVTASIGASNGPLNPLIVTAPTLSFDADGTSKLDLTNSGIITAATVDDAFNWID
                     SGEIFSSLSDSGRSVGYAPVPSSSNIRIRFTLKGDANVDGAVDVGDLGALATNYGVTA
                     GAYWNQGDFNDDGAVNVGDLGSLATNYNQSLGSGSAHATDMAIASESTTAVPEPAMLG
                     LMILSATALPRRRRVRERSS"
     gene            complement(2228..3418)
                     /locus_tag="VLI90_04880"
     CDS             complement(2228..3418)
                     /locus_tag="VLI90_04880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV13570.1"
                     /translation="MATIAETIRPLRIVHYVPGIRLEQGGVVRAILDWCTVFAVRGHQ
                     MTLLAYQGNDIPREWLTGAPGSPVAKVIPPPRAMPGKPLGAEALRVAEQAIKDADVLH
                     LHGPWLDGNRQLANIARRHRVPYIVTLHGMLDDWSMTQRAMKKRLYMRLWGRRFLDNA
                     ACVHCTAEAELAQSRRWFDNPATAVLPYLVELTAFARLPGPGLATPLIPAGMRDRPKV
                     LFLSRLHEQKGIDILIRAAAALRDSGASFTLLIAGNGEPGYERRLYEMVEQLNLRDRV
                     VFLGLVTGEQKVSLYQSADVFVLPTRHENFGLVLTEAMACGTPVVTTKGTDIWSELQS
                     AGGVIAENTPEGIAAAVRGIFQKPQELAALGQRGRAWVFETLAVEPLAQRYEGMYRAQ
                     AARA"
     gene            complement(3515..>4343)
                     /locus_tag="VLI90_04885"
     CDS             complement(3515..>4343)
                     /locus_tag="VLI90_04885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV13571.1"
                     /translation="LVSIHGMLDDWSMAQKSLKKRLYLALVGRRFLNRAARVHCTAEA
                     ELAQARKWFDNSNTAVLPYLVDLTPFETLPGPAAALASLPPAMRHLPKLLFLSRLHEK
                     KGVDVLLRAAARLRDEGRTFALIIAGTGEPAYERSLKELAAQLRLSEIVHFTGLVTGT
                     SKLSLYQAADVFVLPTQQENFGLVLIESLACGTPIVTTRGTDIWQELEQAGGVIAEPA
                     AESIATAIARLLDDPAEQARRGERGRQWTFQTLAVAPLAERYEALYRQIAVRRARHP"
BASE COUNT          789 a         1539 c         1284 g          731 t
ORIGIN      
        1 atccgggtcg tcggagtacc tccgaacccg gcgtggatcg gttcctgacc attctgcaaa
       61 gtgtcggacc gtcagcatgt acccgtgaga gagggttagg gtgagggtga tttcgagcac
      121 tagcggctct ggaaatacaa aatcaccctc accccagccc tctcccacga gtacgtgaga
      181 gagggggaca tgcgtctcgc gctcgttaaa gttgcgccgc cccgttcatc aaacttttgt
      241 gtttctcacc ttttcctcgc tttccccaac cacggcacct cgccccgccg ctataagtga
      301 tcgcttcact tatcgggaac ggggatcaca tgcgcatcta catcgccttg gcggctggat
      361 tggtctgcgt tcgaactgcg acggcgaccg acatcctggt cgagaacttc gacgaacccg
      421 gcgggttctt cgccggcagc ggtttgcaca cgccttcgga tccaccaccc gattggcagg
      481 tggtcaacaa cagtcagccg caggccgcca acagcaacag ctggtttgat gcgccaaacg
      541 ccaactacaa ctatttccag gcgcagtcgg gcgcgaactt cgctggcgtc gatgctgcgt
      601 ccggcagcaa cgtggcgacg ctgaacaact ggctcatcac cccgcaaatc agcttcaacg
      661 ccggcgatca gctttcgttc tactcgcgca gcaagggcag ctatcccgat cgcctgcagg
      721 ttcggctcag cttcaacggc gcgagcgcct ccgccggaac aaccagcacc agcaccggcg
      781 atttttcgac gacgctgctc gacatcaacc cgacgtatct ggccagcaac aacggcggat
      841 atccggcgac gtggacgccg tacacgctca gcttccccgc cgccggcagc gggcggttgg
      901 cgtttcgcta cttcgtcgaa gacggcgggc ccggtggcga caactccgac ttcctcggcg
      961 tcgatacgct cgccgtcgtc acgggcgccc cggccaacca atgggcagcc accaccggcg
     1021 gctcgtggaa caacgccggc aactgggcca gcggcatccc caacgccgcg gcagcgaagg
     1081 tggggcttgg atcgggcgcg acgagtagcg ccgtcgtcac attagacggc aacgtcaccg
     1141 cgggtcagat cacattcaac aacgccaatg gctacagcct acagcccggc gccggcggca
     1201 cactgaaact cgacgacact ggcgacgccg gcggcgttca tccttccgtc agcgtcgtca
     1261 gcggcagtca cttgatttca gcgccgatca cgttgaccag cggcttgacc atcgcgacgt
     1321 ccgccggcgg cgctctgaac atcaccagtg acatcagcgg cagcggcgac atcgccgtcg
     1381 ccggcccggg cacggttcat ttgagcggcg cgctgccgac gacggcggcg atcaacgcga
     1441 acggcgccgt tgtcctcgcc gcccaaacat cgagcggcgc cgcggctcga acgatcgcgg
     1501 cgctcaacat cgcctccggt gtcacggcgt ccatcggcgc atcgaacggc ccgctcaatc
     1561 cgctcatcgt caccgccccc acgctcagct tcgacgccga tggcacatca aagctggact
     1621 tgaccaacag cggcatcatc accgccgcga ccgtcgatga cgcgttcaac tggatcgaca
     1681 gcggcgagat cttcagctcg ctgagcgact ccggtcgatc ggtcggctac gcgcccgtcc
     1741 cctcaagctc caacatcaga atccgcttca cccttaaggg agatgccaac gtcgatggcg
     1801 ctgtcgacgt cggcgacctg ggcgccctag ccaccaacta cggcgtcacc gccggcgcgt
     1861 attggaacca gggcgacttc aacgacgacg gcgccgttaa cgtcggcgac ctcggcagcc
     1921 tcgccaccaa ctacaatcaa tccctcggct ccggttcggc tcatgcaaca gatatggcaa
     1981 tcgcatcgga atcaaccacc gccgtccccg aacctgcgat gcttggcctg atgatcctca
     2041 gcgccaccgc cctcccccgc cgccgccgcg tgcgcgaacg ctcatcctga cggcaggcgt
     2101 acccgccgga gtcgaaacat ttagcccaga agcaagtgaa cgttcggttg gggaaggcca
     2161 cagcagtggc tcgcggtttc gcagagcggt tggcgtagat tgcgaaatcg tgatccggcc
     2221 atcgccctca cgcccgcgcc gcctgcgcgc gatacatccc ctcataccgc tgcgccaacg
     2281 gctccaccgc gagcgtctca aacacccatg cccgcccgcg ctggcccagc gccgccagct
     2341 cctgcggctt ctggaatatt ccgcgcacgg cggcggcaat cccttcggga gtgttttctg
     2401 cgatcacacc gcctgcagat tgcagttcgg accagatatc cgttcccttc gtcgtcacca
     2461 ccggcgtgcc gcacgccatc gcctcggtca gcaccagccc gaagttctcg tgccgcgtgg
     2521 gcagcacgaa cacgtccgcg gattggtaca gcgaaacctt ctgctcgccc gtgaccagcc
     2581 cgaggaagac gacgcggtcg cggaggttca gctgctcgac catctcatac agccgccgct
     2641 cgtaaccggg ctcgccgttg ccggcgatca ataacgtgaa cgacgccccg ctgtcgcgca
     2701 gcgccgcggc ggcgcgaatc aggatgtcga tccccttctg ctcgtgcagc cggcttaaaa
     2761 acaaaacctt cgggcgatcg cgcatcccag ccggaatcag cggcgtcgcc aaccccggcc
     2821 ccggtagccg cgcgaacgcc gtcagctcga cgaggtacgg caacaccgcc gtggccggat
     2881 tgtcgaacca ccgccgcgat tgcgccagct ccgcctcggc ggtgcagtgg acgcacgccg
     2941 cgttatcgag aaatcggcga ccccacagcc gcatgtacag ccgctttttc atcgcccgct
     3001 gtgtcatcga ccagtcgtcg agcatcccgt gcagcgtgac gatgtacggc acgcgatgcc
     3061 gccgcgcgat gttcgcaagc tggcggttcc cgtccagcca gggcccatgc aggtgcagca
     3121 cgtcggcgtc cttgatcgcc tgttccgcaa cccgcagcgc ctccgccccc aacggtttgc
     3181 ccggcatcgc ccggggcggt gggatcactt tcgcaaccgg gctgccggga gcgccggtga
     3241 gccattcgcg cgggatgtcg ttgccttgat acgccagcag cgtcatctga tgcccgcgca
     3301 cggcgaagac ggtgcaccag tcgaggatcg cgcggacgac gccgccttgt tccaggcgga
     3361 tgccgggaac gtagtggacg attcgaagcg gcctgattgt ttctgcaata gttgccatga
     3421 acgcaggccg tcgtcctgtt cgctttgata gccggggccg gaaggcccgg tctttttcca
     3481 cgttcaaaca gaccgggcct tccggcccgg gctattaagg atgtcgcgct cgccgtaccg
     3541 cgatctgtcg atagagcgct tcgtaccgtt cggccagcgg cgcgacggcg agcgtttgaa
     3601 aagtccactg ccgcccgcgc tcaccgcggc gggcctgctc agccgggtcg tccagcaacc
     3661 gcgcgatcgc agtcgcgatt gactccgccg ccggctcggc gatcacgccg ccggcttgtt
     3721 ccagctcttg ccaaatatcg gtgccgcgag tcgtgacgat cggcgtgccg caggcgagcg
     3781 attcgatcag caccaggccg aaattctcct gctgcgttgg cagcacgaac acatcggccg
     3841 cctgatagag cgacagcttg ctcgtgcccg tgacgaggcc ggtgaaatgc acgatctcgc
     3901 tcagtcgaag ctgcgcggcc aattccttca gcgagcgctc gtacgccggc tcacccgtgc
     3961 cggcgatgat cagcgcgaac gttcgcccct catcccgcag tcgcgccgct gcgcgaagca
     4021 gcacgtcaac gcccttcttc tcatgcagcc ggctcaagaa cagcagcttc ggcagatggc
     4081 gcatcgccgg gggcaacgat gccagcgccg ccgccggacc gggcaaggtt tcgaacggcg
     4141 tgagatcgac cagataaggc agcaccgccg tgttcgaatt gtcgaaccac ttgcgcgcct
     4201 gcgccaactc cgcctcggcc gtgcagtgaa cgcgggcggc gcgattgagg aaccgccggc
     4261 caacgagcgc gagatacagc cgcttcttca gcgacttctg cgccatcgac cagtcatcga
     4321 gcatgccgtg gatcgacacg agg
//
LOCUS       DATIMH010000288         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4104863, whole genome shotgun sequence.
ACCESSION   DATIMH010000288 DATIMH010000000
VERSION     DATIMH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4104863"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VLI90_04890"
     CDS             <1..1205
                     /locus_tag="VLI90_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13572.1"
                     /translation="KDITRSIDVVSSPRVAQVLGMFDVPPTSTSSQHWHVDLDLPAHW
                     NVGVIVGPSGSGKTTVARELFNDCIADAFTWPGDRSILDGFPLSLTVRTVVGLLSSVG
                     FSSPPAWVRPFHALSNGQQFRVTLARTLAESMVDGVPRLVDEYSSVVDRTVARIGSAA
                     VAKTVRQYGLKFIAVTCHYDVLDWLEPDWVYEPHTNEFATFASASRECLRRRFKRPPI
                     EMEIIRTDRTAWPRFKPHHYLSGDLHPSAQCYVALLCARRDEDAATPQNHGLEAHATV
                     PVAFTAVLPFPHPTRSGWREHRTVCLPDFQGVGIGNAMSEFIASIYRATGKPYTSTTS
                     HPAMIRHRARSPLWRMRRAPSLVGGHAGGFGSMRKTSAVDRLTAGFEYVGPARIEDAR
                     AFGISIAA"
     gene            1349..1738
                     /locus_tag="VLI90_04895"
     CDS             1349..1738
                     /locus_tag="VLI90_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13573.1"
                     /translation="MRIIAPFNILAAAVGAVVNEVFVHFHASAIEPHEHNPQQVEKLA
                     IIGCTDDDIADRYLVPVELIRTLYPHQLRRGRALHRIGIRQAQWSQTVKQPLVTMLIW
                     LGRNVLGQTNDPERPAEAAPCLESKVG"
     gene            complement(1743..2909)
                     /locus_tag="VLI90_04900"
     CDS             complement(1743..2909)
                     /locus_tag="VLI90_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV13574.1"
                     /translation="MAACASLARVPMARGATAYTATVLYPLQVPAGSASISPRSVMTN
                     HSAGSGLLAGANAPSHALLWSSPTGAASDLNPTNLGDISSSRIMAAVRDQQVGSIGGN
                     ATGGFSHAALWSGAADSAVDLEPTNLSGFFASGGSGSDGVHQVGFGFYGTQASSIHHA
                     LLWTGTADSAVDLNPVKFDANVASLAFGVGGGQQVGEWFSNVNTIHATLWTGSADSAV
                     DLHPADPTLAYSLAIATNGAQQVGAARASGADEQAFLWFGTADSAVNLNPLTLPGINA
                     SEAVATNGQQQVGFGFDGSVNSDDDHALVWSGSADSAVDLHGALPASGTWNFSDADGI
                     DPTGNIFGTANGTYNGFTGDVAVEWSPATLPEPGSSIMLGIGMIYSLRRHRRRR"
     gene            3030..4331
                     /locus_tag="VLI90_04905"
     CDS             3030..4331
                     /locus_tag="VLI90_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13575.1"
                     /translation="MSNALNHSWTPRWAMLRPHAEQLRLWNDEHRFKIVPAGRRSGKT
                     ELAKRRLINHLFRRTWHGKPGRYFAAAPTRDQAKRIWWRDLCALMPRKWIDSVSASDL
                     MIRTVNGSELWVIGLDKPQRMEGVSWDGGVIDEYADCRPGTFDAHIRPALADRRGWLW
                     LIGVPDVDGPAQVEYERFYDIAIKHEDSEWAAFHWASADILPAEEIESAKRRMDPRLF
                     EQEMMGKFILAGGRAFPDFDTKVHVREVEYDPKLPLCWSLDFNINPMCSGIMQHKDGQ
                     VNVLDELVLPDTKTDCAVTAFFDRVAERGWRAQRVQVYGDATGNARDSTSGISDWFIV
                     KNRLRALKPVHKVPSSNPPIKETINAINARLKAADGTVSLAIHPRCTRLIDDFRNALW
                     PSPTMLHDEHSLAWLRYFVHREYPIKLDRPAAAGTLGWSSQ"
BASE COUNT          827 a         1398 c         1539 g          755 t
ORIGIN      
        1 ccaaagacat cactcgatcg atcgacgtcg tctcgtcgcc gcgcgtcgcg caggtcctcg
       61 gcatgttcga cgtgccgccg acttcgactt cctcgcagca ctggcacgtg gacctggacc
      121 tgccggcgca ctggaacgtc ggcgtcatcg tcggacccag cggcagcggg aaaacgaccg
      181 tggcccgcga gctgttcaac gactgcatcg ccgacgcctt cacctggccg ggcgatcgca
      241 gcatcctcga cgggtttccg ctgtcgctca ccgtgcgaac ggtcgtcggg ctgctcagca
      301 gcgtcggctt ctccagtccg cccgcgtggg tgcggccgtt tcatgcgttg tccaacgggc
      361 aacaattccg cgtgacgctg gcgcgcacgc tggccgagtc gatggtcgat ggcgtgccgc
      421 ggctggttga tgagtattcg tcggtcgtcg atcgcaccgt cgcgcggatc gggtcggccg
      481 cggtggcgaa gaccgtgcgg cagtatgggc tgaagttcat cgcggtcacc tgtcattatg
      541 atgtgctgga ttggctggaa ccggattggg tgtatgagcc gcacacgaat gaattcgcga
      601 cgtttgcctc cgccagccgg gagtgtcttc ggcgtcgctt caagcgtccg ccgattgaaa
      661 tggaaatcat ccgcacggat cgcacggcgt ggccgcggtt caagccgcat cattatctga
      721 gcggcgattt gcacccgtcg gcgcagtgct acgtggcgct gttatgcgcg cgaagagacg
      781 aagatgcggc tacgccgcag aatcatgggc tggaagccca tgccacggtg cccgtggcgt
      841 tcacggcggt gctgccgttc ccgcacccga cgcgctcggg ctggcgcgaa catcgcacgg
      901 tatgcctgcc ggacttccag ggcgtgggca tcggcaacgc gatgagtgaa ttcatcgcgt
      961 cgatctatcg cgccacgggc aagccgtata ccagcacgac gagccacccg gcgatgattc
     1021 ggcatcgcgc gcgctcgcca ttgtggcgaa tgcgaagagc gcccagcctc gtcggcggac
     1081 acgcgggtgg gttcgggtcg atgcgaaaga cgagtgcggt cgatcgattg acggcggggt
     1141 ttgagtatgt ggggccggcg cggatcgagg atgcgcgcgc cttcgggatt tccatcgccg
     1201 cgtgactacg aaatccgccg cgtagcggca gtcccgcagg ggcgcgtgtt tttgcgctcg
     1261 aacgaaaaac acgtgcccct gcggggctac cgctacgcaa tggaaagaga gcgcgcgcgc
     1321 acaaccgtga cgtttgccgg gatcgattat gcgtatcatc gcccccttca acatcctcgc
     1381 cgccgccgtt ggcgcggtgg tgaacgaagt cttcgtgcac ttccacgcca gcgcgatcga
     1441 gccgcacgag cacaacccgc agcaggtgga gaagctcgcc atcatcggct gcaccgatga
     1501 cgacatcgcc gaccgctacc tcgtgccggt tgagctgatc cgcacgctat atccgcacca
     1561 gctccgccgc gggcgggcgc tgcatcgcat cggcatccgg caggcgcagt ggtcgcaaac
     1621 ggtcaagcaa ccgctggtga cgatgctgat ctggctgggc cgaaacgtgc tggggcagac
     1681 gaacgacccg gagcgccccg ccgaagcggc gccttgcctg gagtcgaagg tgggatgagg
     1741 cgtcaacgac gccgtcgatg gcgtcgcaac gagtagatca tgccaatgcc cagcatgatg
     1801 gacgaacccg gctcgggaag agtcgccggc gaccattcga ccgcgacgtc gccggtgaat
     1861 ccgttgtacg tgccgttggc ggtgccgaaa atgttgccgg ttggatcgat gccatcggcg
     1921 tcagagaagt tccaggttcc gctcgccggc agcgcgccat gaagatcgac cgcggagtcg
     1981 gcgctgcccg accacaccag cgcgtgatcg tcgtcgctgt tgaccgagcc gtcgaagcca
     2041 aagcccacct gttgctgccc attggtggcc accgcttcgg atgcgttgat gccgggcaac
     2101 gtcagcggat tcaaattgac ggcggaatcg gccgtgccga accagaggaa tgcctgctcg
     2161 tcagcgccgc tggcgcgggc ggcgccgacc tgctgcgcgc cgttggtggc gattgccagt
     2221 gaataggcga gcgtcggatc agccggatgc agatcgacgg cggaatccgc cgagccggtc
     2281 caaagcgtgg cgtgaatcgt gttgacgttg gagaaccatt caccgacctg ctgcccgccg
     2341 cccacaccga acgccagcga cgcgacgttg gcgtcgaact tcaccgggtt caaatcgacg
     2401 gcggaatcgg cggtgccggt ccacagcagc gcgtgatgaa tggaactcgc ttgcgtgccg
     2461 taaaagccaa agcccacctg gtggaccccg tcgctgccgg agccgcccga ggcgaaaaag
     2521 ccgctcaggt ttgtcggctc cagatcgact gctgaatcag cggcgccgct ccacagggcg
     2581 gcgtgggaaa acccgccggt ggcgttgccg ccgatcgatc caacctgctg gtcgcgcacg
     2641 gccgccatga tgcgcgacga gctgatgtcg ccgaggttcg tcggattcaa atcgctggcc
     2701 gcgccggtgg gtgatgacca cagcagcgca tgactgggcg cgttggcgcc ggcgaggaga
     2761 ccggaacccg cggaatggtt ggtcatcacc gaacggggcg aaatgctggc agaaccggct
     2821 ggaacctgca gcgggtacag cacggtcgcg gtgtaggccg tcgcgccgcg cgccatcgga
     2881 acgcgcgcca gcgaagcgca ggcggccatc cctgcgcaag aaataagaat tgaacggcgg
     2941 ggcatcagcc ccctccctga atatcggata tcgggccgcg caattttatc tctgccggcg
     3001 aactcaaaca acgggagttt ctcaaaccca tgtccaacgc gctcaaccat tcctggacac
     3061 cgcggtgggc gatgctccgc ccgcacgccg agcaactgcg gctgtggaac gacgagcatc
     3121 gattcaaaat cgtcccggcc gggcgacgca gcggcaagac cgagctggcc aagcgccggt
     3181 tgatcaacca cctgttccgc cggacctggc acggcaaacc ggggcgctat ttcgccgcgg
     3241 cgccgacgcg cgaccaggcc aagcgcatct ggtggcgcga tctgtgcgcg ctgatgccgc
     3301 ggaagtggat cgacagcgtg tcggcgtcgg acctgatgat ccgcacggtt aacggctcgg
     3361 agctgtgggt gatcggcttg gataaaccgc agcggatgga aggcgtcagc tgggacggcg
     3421 gcgtcatcga cgagtacgcc gactgcagac cggggacgtt cgatgcgcac atccgccccg
     3481 cgctggcgga ccgccgcggg tggttgtggt tgatcggcgt gcccgacgtc gatgggccgg
     3541 cccaggtgga atatgagcgc ttctatgaca ttgctattaa acacgaggac tctgaatggg
     3601 cggcgttcca ctgggcgtcg gccgacatct tgccggcgga ggagatcgaa tcggccaaac
     3661 gccgcatgga cccgcggctg ttcgagcagg agatgatggg gaagttcatc ctggccggtg
     3721 ggcgggcgtt tccggatttc gatacgaagg tgcatgtgcg cgaggtcgaa tatgacccga
     3781 agttgccgtt gtgctggtcg ctcgacttca acatcaaccc gatgtgcagc gggatcatgc
     3841 agcacaagga cggccaggtg aacgtgctgg acgagctggt gttgccggac acgaagaccg
     3901 attgcgcggt gacggcgttc ttcgatcgcg tcgccgagcg cgggtggcgc gcgcagcgcg
     3961 tgcaggtcta cggcgatgcc accggcaacg cccgcgacag caccagcggg atcagcgact
     4021 ggttcatcgt gaagaaccgc ctgcgggcgt tgaagccggt gcacaaggtg cccagcagca
     4081 atccgccgat caaggaaacg atcaacgcga tcaacgccag gttgaaggcc gcggacggca
     4141 cggtgtccct ggcgattcac ccgcggtgta cgcggctgat cgacgacttc cgcaacgcgc
     4201 tgtggccgag cccgacaatg ctgcacgatg agcattccct cgcgtggctg cggtatttcg
     4261 tgcaccgaga gtatccgatc aagctggatc ggcccgcggc ggcggggacg cttgggtggt
     4321 cgtcgcagtg atctttgtcg cgccgaaaaa atgcgagcgc gaagtcgcga aggcgccaag
     4381 acagacgcgg aggggaatga ttgtcatcct ccagcgcagc acgcactcgt gcggagtcga
     4441 tggatctagc tgaaaaggaa gagaaggatc actggcgcga atggcgcggc cgtcgcctgc
     4501 ggtttcgcag accaggtcg
//
LOCUS       DATIMH010000289         3719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5552060, whole genome shotgun sequence.
ACCESSION   DATIMH010000289 DATIMH010000000
VERSION     DATIMH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5552060"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2629)
                     /locus_tag="VLI90_04910"
     CDS             complement(<1..2629)
                     /locus_tag="VLI90_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSV13576.1"
                     /translation="MSTNSNKRQLTSGISAVAIAVALGFASPAHAQQEFAILQGHVDG
                     AAPGTQVVAVDTQNGRRSVGTVDARGNYEIFGLRPSTYSVSVAGRAAQTATLQVGQTV
                     ALDFIAARANGNIVVTGRRTAQPVQAQTVATNITPAQIENLPQNSRNFLSFATLAPGI
                     TLTNPSGAVQFQSGAVTADHSNVILDGMSFKNPVNHGGMFGQNFGDFGNPFPQIAIQE
                     YQVETQNFGAEVGQSAGAVLNAITKTGGDKFHGSIFGEWQPKALIEKPFFQRNQPKQD
                     FDRKQFGGELGGPIIPGVLSFYVGGEGTIQRLPAVTFNVNATVPPNIEALVNTAKPKT
                     FKQGLYFGKLTWFATPDDTVNGTVYVRRQSNLSDYGGNAVASHGHLLTTDQTRYQLQW
                     KHTGGNFLNYVNIAHVKAVNSTPTVSQGPEFILTAPPTVVNGAATGTGATQVLLGNNS
                     FVQGDTEKSWMFKDDATYRAGTHTLKAGAQVTFYNLSREVNDHFNGTYYVANPCVGTT
                     CPANFDFAAAQPYWGQINIQASPTLAGKDTLVGIYAEDEWKPDAHWTVNYGLRWDFES
                     NPNDVHYVTPPAVAAALRGYQGWQARGINANDYISTGNNRHPRYNEFQPRVGVAYDVH
                     GDHDFVVFGGAGRYYDESLFIEGQIEQQMNSSIIIGQALPACAGAAPPSYCHDPNALR
                     TFFQSQGFTGGAVWVLPNKIKTPYSDQFDLGVRKQFGVFQTSLTYSHVESHNLFLFAR
                     SNFYNNGWASVLRTPAGCVNGGDNWINDNVPNGPFPNCAVSGAQLPGFNGKVDRGLDN
                     GRARLDALYLHIEKPFTNTSVWGVTESFTLQRARTNVGQDPFNQDEMFNGTELNVFGW
                     NYLPEVPKWSSVTSATWRAP"
     gene            complement(2804..>3719)
                     /locus_tag="VLI90_04915"
     CDS             complement(2804..>3719)
                     /locus_tag="VLI90_04915"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HSV13577.1"
                     /translation="GDALSVGYQDWNQWLPCNRAQAVPCDSVEPVTPYTRSTARKAGW
                     QWRIPLQHRIGNGYVYASELISDEEVAESLLANLDGKATADPRPVRFRPGRRLKSWDK
                     NVIAIGLSSGFLEPLESTSIHLIQTGIHRLLAMFPAVRFSHADIDEYNLQARTEYEDV
                     RDFIIAHYKVTRRTGDPLWDHVRTMDVPDSLNARLELFRSSGRFFKHNAQELFAEESW
                     VQVLLGQGFEMRADPVTQFVPDDDLAGFLGDLAEVIEDVASTMPDHGEFVRGLPPSTD
                     AARSQQQAAAAASTLDFTLRYERGETLA"
BASE COUNT          657 a         1115 c         1219 g          728 t
ORIGIN      
        1 acggcgcccg ccaggtagcc gacgtcaccg agctccattt cggaacctcc ggcaggtagt
       61 tccagccgaa gacgttcagt tccgtgccgt tgaacatctc gtcctggttg aacgggtcct
      121 gcccgacgtt tgtcctcgcc cgctgcaggg tgaaggactc ggtgacgccc cagacgctcg
      181 tgttcgtaaa cggcttttcg atgtgcagat aaagggcgtc caagcgagcg cgcccgttgt
      241 ccagcccgcg atcgactttg ccgttgaatc caggcagctg agcgccgctt accgcgcagt
      301 tcgggaacgg gccgttcggg acattgtcgt tgatccaatt gtcgccgccg ttcacgcacc
      361 cggccggggt gcgcagcacg gaagcccagc cgttgttgta gaaattggac cgcgcgaaca
      421 ggaacaggtt gtgcgattcg acatgcgaat aggttagtga tgtctggaac acgccgaact
      481 gcttgcgcac gcccagatcg aactgatctg aatagggcgt cttgatcttg ttcgggagca
      541 cccagacggc gccgccggtg aacccctggc tctggaagaa ggtgcgcagg gcgttcggat
      601 catgacagta actgggcgga gccgcaccgg cgcaggcagg aagagcctgc ccgatgatga
      661 tgctgctgtt catctgctgc tcgatctgcc cctcgatgaa cagcgactcg tcataataac
      721 ggccggcccc gccgaacacg acgaaatcat ggtcgccatg aacgtcgtac gccacgccga
      781 cacgaggctg gaactcgttg taacgcggat gccggttgtt gccggttgaa atatagtcgt
      841 tcgcgttgat gccgcgcgcc tgccagccct ggtaaccccg cagcgccgct gcgaccgcgg
      901 gcggcgtcac ataatggacg tcgttcgggt tactctcgaa atcccagcgc agcccgtaat
      961 tcacggtcca gtgggcatcg ggcttccact catcctcggc gtaaatgccg acgagcgtat
     1021 ccttgcccgc gagcgtcggc gaggcctgga tgttgatctg gccccagtaa ggctgggcgg
     1081 ccgcgaaatc gaagttggcg gggcaagtcg tcccgacgca cgggttggcc acataatatg
     1141 tgccgttgaa gtgatcgttc acctcacgcg acaggttgta gaaggtgacc tgtgcgcccg
     1201 ccttcagcgt atgggtgccg gcgcggtagg tcgcgtcgtc cttgaacatc catgacttct
     1261 cggtgtcgcc ctggacgaag ctgttgttgc cgaggaggac ttgggtcgca ccggtgcccg
     1321 tcgcggcgcc gttcaccacc gttggaggag cggtcaggat aaactccggt ccctgcgaaa
     1381 ccgtcggcgt cgaattcacc gccttgacat gcgcgatgtt cacatagttg agaaagtttc
     1441 ccccggtatg cttccattga agctggtagc gggtctggtc ggtcgtgagc aggtggccgt
     1501 ggctggccac cgcgttgccg ccataatcgc tgagattgct ctggcggcga acgtacaccg
     1561 tcccgttgac ggtgtcgtcg ggcgtcgcga accaggtgag cttgccgaaa taaaggcctt
     1621 gtttgaacgt cttgggcttc gcggtgttga ccagcgcctc gatattggga ggcacggtcg
     1681 cgttcacgtt gaaggtgacc gcgggcaatc tttggatcgt tccttcgccc ccaacgtaga
     1741 acgacagcac gccggggatg atcggtccgc cgagctcacc gccaaactgc ttccggtcga
     1801 agtcctgctt gggctgattg cgctggaaga agggtttctc gatcagggcc ttgggctgcc
     1861 actcgccaaa aatcgagccg tgaaacttgt cgccgccagt cttggtgatc gcgttgagca
     1921 cggcgcccgc cgactgaccg acctcggcgc cgaaattttg tgtttcgacc tgatattcct
     1981 ggatggcgat ctgcgggaac gggttgccga agtcgccgaa gttctggccg aacatgccgc
     2041 cgtgattgac cgggttcttg aagctcatcc cgtccaggat gacgttcgag tgatcggcgg
     2101 tcacagcgcc tgactggaac tggactgcgc cgctcggatt ggtcagcgtg atgcccggcg
     2161 ccagcgttgc gaagctcagg aagttgcgcg agttctgcgg caggttctcg atttgcgcgg
     2221 gagtgatgtt ggtggccacc gtctgcgcct gaaccggctg cgccgtgcgg cggccggtca
     2281 cgacaatatt gccgttggcg cgcgccgcga tgaagtcgag cgcgaccgtc tggcccactt
     2341 gaagggtggc ggtctgcgcg gcgcgaccgg caacggacac cgaataggtc gacgggcgaa
     2401 ggccgaagat ctcgtaattg cccctcgcat cgaccgtgcc gacggatcgc cggccgttct
     2461 gcgtatcgac agcgaccacc tgcgtgcccg gcgccgcgcc gtcgacgtgg ccctgcagaa
     2521 tcgcaaattc ctgctgcgca tgcgcaggcg aggcgaagcc cagcgccacc gcgatcgcga
     2581 cagcggaaat gccagacgtg agctggcgct tgttcgaatt ggtgctcacc cttgttccct
     2641 ctcccaaggc cggtgatgta accggttaca cgacgatacg agctgcttgc ctctttgccg
     2701 cggaagaaac gccttcggca gcttggagtc aattacgggt cagcaccggc gggtgccggt
     2761 ttaggaacgg ctgtggcttt cgcgcaacgc ggcggcaaca agctcaggcg agcgtttcgc
     2821 cgcgttcgta gcgcaaggtg aagtccaggg tcgatgcggc cgccgcggct tgctgctgag
     2881 agcgtgcggc atctgtgctc ggaggcagcc cgcggacgaa ttcgccatgg tcgggcatgg
     2941 tcgaggcgac atcttcgatg acttcggcga gatcgccgag gaagccggcc aggtcgtcgt
     3001 cgggaacgaa ctgcgtgacg ggatcggcgc gcatctcaaa gccctgcccc agcagaacct
     3061 gcacccagct ctcctcggcg aaaagctcct gcgcgttgtg cttgaagaac cggcctgacg
     3121 agcgaaacag ctccagtcgc gcattgaggc tgtccggcac gtccatcgtg cggacatgat
     3181 cccacaacgg atcgccggtg cggcgcgtga ccttgtagtg agcgatgatg aagtcgcgca
     3241 catcctcata ttcggtgcgc gcctgaagat tatattcgtc gatatcggcg tgcgaaaacc
     3301 ggaccgcggg gaacatcgcg agaaggcggt ggatgcccgt ctggatgagg tggatgctgg
     3361 tcgactcgag cggctcgagg aaaccagacg agaggccgat ggcgatgacg ttcttgtccc
     3421 acgacttgag ccggcgtccg ggcctgaacc gcaccggcct gggatcggcc gtggccttgc
     3481 cgtcgaggtt cgcgagcaat gattcggcga cttcttcgtc gctgattagc tcggatgcat
     3541 agacgtagcc gttgccgatc cggtgctgga gcgggattcg ccattgccac cccgccttgc
     3601 gcgcggtgga tcgcgtgtaa ggggtgaccg gctcgacact gtcgcaaggc acagcctggg
     3661 cgcggttgca cggcagccat tggttccagt cctggtagcc gacactgagc gcgtccccg
//
LOCUS       DATIMH010000290         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3241614, whole genome shotgun sequence.
ACCESSION   DATIMH010000290 DATIMH010000000
VERSION     DATIMH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241614"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VLI90_04920"
     CDS             complement(<1..320)
                     /locus_tag="VLI90_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13578.1"
                     /translation="MAKSTPRTRRPAPPQSDAADTREQDQPQTAAATAVEAPPAPAPR
                     AERPAPPERQERPERQDRGDRGDRNESSGGGGGGGGSVGEDVAGIDAETNAKYEQVKG
                     GKLY"
     gene            complement(915..1526)
                     /gene="coaE"
                     /locus_tag="VLI90_04925"
     CDS             complement(915..1526)
                     /gene="coaE"
                     /locus_tag="VLI90_04925"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HSV13579.1"
                     /translation="MFHNKPVIGIAGGIGGGKTHIAKLFGELGCFIIHSDDQVRDAYN
                     DPAVQDAIRTWWGDDVFRADNTVNRSAIAALVFNDPAERRRLEQLLHPWVNDARERAM
                     SAVADDPQVLAFIWDTPLLFETALNRQCDAVVYVETPAQARLQRLTATRGWDAGELAR
                     REKSQWALDKKREMSDYVLVNTADADDAVRSQVREILSRILAK"
     gene            complement(1762..2214)
                     /locus_tag="VLI90_04930"
     CDS             complement(1762..2214)
                     /locus_tag="VLI90_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13580.1"
                     /translation="MERLELEPSTGLQPRTDPRPRMDELPPVRLVAVEDVRLPTPPGL
                     GEQLDSFYVGLWQFERDVTEGGGAIVYRAENVRLRFEVVADQKPIERETLRPQGIEVL
                     SLAEAELKLVEAEHEYIRQRGLTPGQESLLLQDPAGNWIELFEVRRVG"
     gene            complement(2249..2626)
                     /locus_tag="VLI90_04935"
     CDS             complement(2249..2626)
                     /locus_tag="VLI90_04935"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV13581.1"
                     /translation="MKRAKKTSASERVVIHGSGNVFADIGFAPDEAPELSVKAELTRQ
                     IYNRIRRLGLTQVRAADRLGISQPDVSKLMNGRHTGYSIERLISLLNALAMDIDIVVR
                     VRESARGRQRGVLRILEATRVAG"
     gene            complement(2613..3014)
                     /locus_tag="VLI90_04940"
     CDS             complement(2613..3014)
                     /locus_tag="VLI90_04940"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSV13582.1"
                     /translation="MAEEGSGGAKWRPVHWIGRSLADLKEFPVLAQRNIGFALWFAQR
                     GDKHPSAKPAKWFGGSGVLEIVEDLHGDTYRAIYTVRFAKSIYVLHAFKKKSKSGIKT
                     PRHELALIDSRLKWAAADYQRWLKEQANEES"
     gene            complement(3071..>3730)
                     /locus_tag="VLI90_04945"
     CDS             complement(3071..>3730)
                     /locus_tag="VLI90_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HSV13583.1"
                     /translation="LRMLAHFTQEPALMKAFAADEDIHKAVAAEVYNVPLADVTREQR
                     GYAKTINFGIIYGVSAFGLAARIEGLTQQSAQELIITYNKRFPGIETFSKQQVQHAQA
                     YGYVETILGRRRPITEINSAIIAQRRAAERAAGNSVIQGSAADLIKKAMVSLHRRMKK
                     EKMQSRMLLQVHDELVFETPAEVAEREGEIIREEMANAMTLTVPLKVEVGWGKNWDDA
                     K"
BASE COUNT          677 a         1269 c         1042 g          742 t
ORIGIN      
        1 atgtagagct tgccgccctt gacctgttcg tatttcgcgt tggtttcggc atcgatgccg
       61 gcgacgtctt cgccgaccga accaccgcca ccaccaccgc cgccggacga ttcgttgcga
      121 tcgccacggt cgccgcggtc ctggcgctcg gggcgctcct ggcgttccgg tggcgccggg
      181 cgctcggcgc gcggtgcggg agccggtggg gcttcgacgg ccgttgcggc cgcggtctgc
      241 ggttggtctt gttcacgggt atcggcggcg tcgctctgcg gcggcgccgg ccggcgggta
      301 cggggtgtgg acttagccat ctggctagcc tccaaaagaa ttcaaagaag gaatgaacgt
      361 ttcgtgcggg tctcgtgtgt gaagaagatt cagttgaagt tccgcttttc acggcggatc
      421 acgcgcggtt cacgcgtgcg acggttcaaa cgcgcccggt ggacatgctg aacgaacaac
      481 tgagggaact tagccgcgag gggctacata caactctacg gcaacaatcg ccccaaactc
      541 aactcgattc tcgcaggaaa ataattcgtt tctgtgctcg gcagaacaat cgcggccaag
      601 cgacgcgcgt gcgcgatttg tgaaacacac gccaaacgca atgcacacat ccgcccgcgc
      661 accggggcga atcgcgcgcc gaacaaatcg tcttgtcgtg gggagatcaa ccagtcatct
      721 tacgcccgct tccccgcggg cgtccacgca tgcagcggcg agcatccggc acgccgcata
      781 ctttctcaca tccacccaaa gctgaaacgg accgctcgtc acacgccatc atggcgtgat
      841 tgctcgccgt cgccgcacct ggattcaacc gtgtggggat caaacccgcg gaccattcgg
      901 tccggttgcg gtcgtcactt cgcgagaatt cgggaaagta tctctcggac ttgactgcgg
      961 acagcgtcgt ccgcgtcggc ggtgtttacc aagacataat ccgacatttc ccgcttcttg
     1021 tccagtgccc actgtgattt ttctcgccga gccagttccc ccgcgtccca accgcgggtg
     1081 gccgtcaaac gctgcaaccg agcctgggcc ggggtctcca cgtacaccac cgcgtcacac
     1141 tgccggttaa gtgccgtttc aaacagcagc ggcgtatccc aaatgaaagc aagcacttgc
     1201 ggatcatccg ccaccgcaga catcgcccgt tctcgggcat cgttcaccca gggatgcaac
     1261 agttgttcca agcggcgccg ctccgcagga tcattaaaaa ccagcgccgc gatggccgag
     1321 cgattcaccg tgttgtcggc ccggaagacg tcgtcgcccc accatgtgcg gattgcatcc
     1381 tgcacggccg ggtcattata cgcgtcgcgc acctgatcgt cggagtggat gatgaagcag
     1441 ccaagttcgc cgaaaagttt ggcgatatgc gtcttccccc cgccgatgcc gcccgcgatg
     1501 ccgatgaccg gtttgttgtg gaacacgcga acgcgattgt agatgaccga agccccgcgt
     1561 gtacacgctg aggatccgac ccttttgcag ggacggtcag caccgcgccg ggttcggagc
     1621 gtacccgcga ccacccggat tttcctgtag cgcgaaaaaa aatccgcttc gtcggaatac
     1681 ctccgaaccc ggcgtggttt gatcacgaca tcgcccgtcc aaaggtgtcg ggcgttcagc
     1741 acgtacatgt ggggctccgc ctcaaccgac tcgccgaact tcaaacagct cgatccaatt
     1801 ccccgccggg tcctgcagca gcaggctttc ctgcccgggc gtcagcccgc gttggcggat
     1861 atattcgtgc tccgcttcga ccagcttcaa ctccgcctcc gccagcgaca gcacttcgat
     1921 cccctgcggc cgcagcgtct cgcgctcgat cggcttctga tccgcgacaa cctcaaaccg
     1981 cagccgaacg ttctccgcgc gatagacaat cgccccgccg ccttccgtca cgtcgcgctc
     2041 gaattgccaa agcccgacgt agaacgagtc gagttgctcc cccaaccccg gcggtgtcgg
     2101 cagccgcacg tcctccaccg ccaccaagcg caccggcggc aactcgtcca tccgcggccg
     2161 cggatctgtg cgcggttgaa gaccggtcga tggttcaagc tccaggcgtt ccataccaga
     2221 tcatcattga ccagcacgcg gccacacttc aaccggccac gcgcgtcgct tccaaaatcc
     2281 gcagcacgcc gcgctgccgc ccccgggccg attcccgtac tcgcaccaca atatcgatgt
     2341 ccatcgccag cgcattgagc aacgagatca aacgctcgat cgaataaccg gtgtgccggc
     2401 cgttcatcag cttcgaaacg tccggctggc tgatgccaag gcgatcagca gcacgcacct
     2461 gcgtcagccc aagtcgccga atgcggttgt aaatctgtcg cgtcagttcg gccttcacgc
     2521 tcagctccgg tgcctcgtcc ggggcaaatc caatgtcagc gaaaacattg ccgctcccat
     2581 gaatcacaac tcgttcggat gcggatgtct ttttagctct cttcatttgc ctgctccttc
     2641 agccaccgct ggtagtccgc cgccgcccat ttcagccggg agtcgatcaa cgccagctca
     2701 tgccgcggcg ttttgatgcc gctcttgctc ttcttcttga atgcgtgcag cacgtaaatc
     2761 gacttggcga atcgaacggt gtaaatcgcg cggtacgtat caccgtggag gtcttcgacg
     2821 atctccaaca caccggaccc accaaaccac ttagccggct tcgcgcttgg gtgcttatcg
     2881 ccacgctgcg cgaaccagag ggcgaagccg atgttccgct gcgccaggac tggaaactcc
     2941 ttcaggtccg caaggctgcg gccgatccag tggaccggac gccacttcgc cccgccggag
     3001 ccttcctcag ccattatggc tattccacca tatggccgct ggcgtcacaa ctgtggatag
     3061 gattcctgcc ctacttcgca tcatcccaat tcttccccca tcccacctcc accttcagcg
     3121 gcaccgtcag cgtcatcgcg ttggccatct cttcgcggat gatttccccc tcgcgctcgg
     3181 ccacttccgc gggagtctcg aacaccaact catcgtgcac ctgcagcagc atccggctct
     3241 gcatcttctc cttcttcatc cgccggtgca ggctcaccat cgccttctta atcaagtcgg
     3301 cggcggagcc ttgtatcact gaattacccg cggcacgctc ggcggcgcgg cgctgggcga
     3361 tgatggcgga attgatctcc gtgatcggcc ggcgccggcc gaggatcgtc tcgacgtagc
     3421 cgtacgcctg cgcgtgttgc acctgctgct tcgaaaacgt ttcgatgccg gggaaccgct
     3481 tgttgtaggt gatgatcagc tcctgcgccg actgctgcgt taggccttcg atgcgagcgg
     3541 ccaggccgaa cgcgctgacg ccgtagatga tgccgaagtt gatcgtcttc gcgtagccgc
     3601 gctgttcgcg cgtcacgtcc gccagcggca cattgtagac ttccgcggcg acggccttgt
     3661 gaatgtcttc gtccgccgcg aacgctttca tcaacgccgg ttcctgcgtg aagtgcgcga
     3721 gcatgcgcag
//
LOCUS       DATIMH010000291         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_66221, whole genome shotgun sequence.
ACCESSION   DATIMH010000291 DATIMH010000000
VERSION     DATIMH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66221"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VLI90_04950"
     CDS             <1..762
                     /locus_tag="VLI90_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV13584.1"
                     /translation="FVLVALGLFLIHNLIDFSMFEVGPLFLFLMLVGAALGVAPATAR
                     AGRSRAWAIAILILAIIGWLVAAARLVGPVVVAEQSAFEADEAIRTAPADQPAQLEMR
                     YRQAATGLSDALARVPWNTDYALRAAKAWLGAGQVDRAEDMLAAAIASDPMYIDAYLL
                     RANLELQQPSPDVARVRSDFGTIIRLNPNDVSFHRHYGEALERFGLKDAAREQYELAL
                     KYNSELAEGEPKRMSAAQEEELTARIARLSGEHGR"
     gene            835..1764
                     /locus_tag="VLI90_04955"
     CDS             835..1764
                     /locus_tag="VLI90_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSV13585.1"
                     /translation="MGKQRNKLIDLSQYVGLRVVNMLLHSFPVDANLRTARLLGNFMY
                     AFDRKHRDRAMGNLRRSFPEMPERQRQLLARRSMQQLFMLFIEVLFTTRLVHLDTWAD
                     YCELENFREVLQLLLKRDQGLIMLTGHFGNWEILGYVLATLGFNTTSIARPLDNPYVN
                     RWLLGVREKKGQRIIAKKGATEEVIEVLARGGAVGFIADQNAGSKGIFVDFFGRKAST
                     YKSIGLLAMQYEVPIIIGYARRVDDSFKFRVGAQDIIYPQDWKDQDDPLRYITQRYTK
                     AIEDFVLKDPGQYLWIHRRWKTRPKGETAETFD"
     gene            complement(1785..2531)
                     /locus_tag="VLI90_04960"
     CDS             complement(1785..2531)
                     /locus_tag="VLI90_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13586.1"
                     /translation="MTDSPADDASAMPTASLPLVPTLGYATTATSIDPPLPSGPRPWW
                     VYGVVAIYLLLLGGMLTMPIWAAFTDGNDVVLPAAIFVAALFGCGWTLILLPVRALRL
                     RPVRRRSIWLPVIVSGLLGGALVFGAGIALYEFFRISGDAVEWALVLGAVCVWAGWSI
                     VFMLIVGSNGPGTIGMKLHRLLIAGSALELLIAVPAHVVVRRRNECCAGIETGIGICI
                     GVGVMFISFGPSVLVLYHRRRKRITPPRRR"
BASE COUNT          519 a          829 c          923 g          484 t
ORIGIN      
        1 ttcgtgctcg tcgcgctggg actttttctg atccacaacc tgatcgactt ctcgatgttc
       61 gaggtcgggc cgctgttttt gttcctcatg ttagttggcg cagcgctggg cgtggcgccg
      121 gcgacggcgc gagcgggtcg atcgcgcgcg tgggcgatcg cgatattgat cctcgcgatc
      181 atcggttggc tcgtcgcggc cgcccggctc gtcggtcccg ttgtcgtggc ggagcagtcc
      241 gccttcgaag cggatgaggc gatccgcacc gcgccggcgg atcagccggc gcagttggaa
      301 atgcgttatc gacaggcggc gacgggattg tctgatgcgc tggcgcgggt gccgtggaat
      361 acggactatg cactgcgcgc ggcgaaggcg tggctcgggg cggggcaggt cgatcgggcc
      421 gaggacatgc tggcggcggc gatcgccagc gatccgatgt acatcgatgc gtacctgctg
      481 cgggcgaacc tggagctgca gcagccttcg ccggacgtgg cgcgcgtgcg atcggacttt
      541 ggcacgatca ttcggttgaa cccgaatgat gtgtcgtttc atcggcatta tggggaggcg
      601 ctggagcggt ttggattgaa ggacgcggcg cgcgagcagt atgagttggc gttgaaatac
      661 aattcggagc ttgcggaggg ggagcctaag cggatgtctg ccgcgcagga ggaggagttg
      721 acggctcgga tcgctcgatt gagtggggag catggtcgct gaagcgaccg cgccgcgcgt
      781 ttgggggcgg cgggcatcgc ctctgaagtt cgcagccgct atcatcgccg ccccgtggga
      841 aaacagcgca acaagctgat cgacttgtcg cagtacgtcg gcctgcgcgt cgtcaacatg
      901 ctgctgcaca gttttccggt cgatgcgaac ctgcgcacgg cccggttgct gggcaacttc
      961 atgtacgcct tcgaccgcaa gcatcgcgac cgcgcgatgg ggaacctgcg gcgaagtttt
     1021 ccggaaatgc ccgagcggca gcgccagctg ctcgcccgcc ggagcatgca gcagttgttc
     1081 atgctgttca tcgaggtgct gttcaccacg cggctcgtcc acctcgacac ctgggcggac
     1141 tattgcgagc tggaaaattt ccgcgaggtc ctgcagctgc tgctcaagcg cgaccagggc
     1201 ctgatcatgc tgaccggtca cttcggcaac tgggaaatcc taggttacgt gctagcgacg
     1261 ctcggcttca acaccaccag cattgcccgg ccgctcgaca acccatacgt caaccgatgg
     1321 ctgctcggcg tacgcgagaa gaagggccag cgcatcatcg ccaagaaagg cgcgaccgaa
     1381 gaagtgatcg aagtccttgc ccgcggcggc gctgtcgggt tcatcgcgga tcagaatgcc
     1441 ggcagcaaag gcatcttcgt agacttcttc ggccgaaagg cgagcacgta caaatcaatc
     1501 ggactgctcg cgatgcagta cgaagtgccg atcatcatcg gctacgcacg ccgcgttgac
     1561 gacagcttca aattccgcgt tggggcgcag gacatcatct acccgcagga ttggaaggac
     1621 caggacgacc cgctgcgcta catcacgcag cggtatacga aggcgatcga agattttgtg
     1681 ctcaaggatc cgggccagta cctgtggatt caccggagat ggaagacgcg ccccaagggc
     1741 gagacggcgg agacgttcga ttgagcaagc gaggcatgtc cgcttcagcg gcgacgtggc
     1801 ggcgtgatgc gcttgcgccg gcggtggtac agcaccagca cgctgggccc gaaggagatg
     1861 aacatcaccc cgacgccgat gcagatgccg atgccggttt cgatgccggc gcagcattcg
     1921 ttccgccggc ggaccaccac gtgcgcgggc acggctatta gcaactccag cgcgctgccg
     1981 gcgatcagca ggcggtgcag cttcatgccg atcgtcccag ggccgtttga tccgacgatc
     2041 aacataaaga cgatcgacca ccccgcccac acacagacag cccccagcac gagcgcccat
     2101 tccaccgcgt cgccgctgat cctgaagaat tcgtaaagtg cgattcccgc tccgaagacg
     2161 agtgcgccac cgagcaaacc ggagacgatg acgggcagcc agatcgatcg acgccggact
     2221 ggccgcaggc gaagcgcccg caccggcagc aggatcagcg tccacccgca accgaagagc
     2281 gccgcgacga agatggcagc cggcaggacg acatcgttgc cgtcggtgaa cgcggcccag
     2341 atgggcattg tcaacatgcc gcccagcagc aggagataga tcgcgacgac gccgtacacc
     2401 caccacggcc gcggcccgct ggggagcggc gggtcgatcg acgtagcggt ggtcgcgtag
     2461 ccgagcgtcg ggaccagcgg caagctcgcg gtgggcatgg cgctggcgtc atcagctggg
     2521 gagtcggtca ttttttcttt cgtcgaagaa gatcatcgcg gtagcgcggt agccccgaag
     2581 gggcacgtgt ttcgtggcac ggtgcctcgc ccgtgtttcg tggtactcca cgaatatcgg
     2641 cggtactccg tcgatgaagc aatcgcgtta gcgaatagat gtcgaccacc gtcgcttgcg
     2701 cgattgctag gtcgatgagt acattgcccg tgcgtgagta cacgcaccac gggcc
//
LOCUS       DATIMH010000292         2331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_645305, whole genome shotgun sequence.
ACCESSION   DATIMH010000292 DATIMH010000000
VERSION     DATIMH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645305"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VLI90_04965"
     CDS             <1..681
                     /locus_tag="VLI90_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV13587.1"
                     /translation="DVGPVSTSPLIVNVSGGTAAFASTQHLSAVNVSGAGLAQMSAAA
                     TPAAANVLNVTSISFTGASAKIDLTNNEMITTTTPAVIRGDIAAHSLITSITTPLGRN
                     LGYKDLNGTQTEVRYTLLGDTDLNGAVNVGDLGALATNYGTTAGALWSQGDFNYDAKV
                     DVADLGALATNYNVALGFGAGSATVAIAEPAASTAAAIPEPASVVLIAAGALGLCLRR
                     QRRLRVRP"
     gene            complement(755..1099)
                     /locus_tag="VLI90_04970"
     CDS             complement(755..1099)
                     /locus_tag="VLI90_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13588.1"
                     /translation="MTNATKRSILRWVHLIVTIPILGYIYGKPSDVEQYAGGVRYLFV
                     PIIILSGYWMYGGIIFAMLGAALWLGAFRLSGFGAALLSQIALFIGRKIWLMMRARRR
                     QADIGLTEAVET"
     gene            1272..>2331
                     /locus_tag="VLI90_04975"
     CDS             1272..>2331
                     /locus_tag="VLI90_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13589.1"
                     /translation="MHLRTKHLTLATLLAALAAASGVARTANADSIWIGATTGTPIKA
                     DGIKVLRIEGDSLAYTTPSGSQTSKPLSQIAQMASDDEPAFNAAEQAYRDGKWADAVD
                     GYQKAVQTTAKDWVRDRSSIRLVDVAAKTNRFDAAVTAYVALVQKDPTLAAKAKPALP
                     DANSKYLDGAIADINKALESTRLSDAQRSSLLSFELDIYRARKDTANVNQTLQQLVKL
                     GAATPADLAMLKLASAHAALDAKEYSKAIGEIEQNRKLFMDPANQLDALQTLAQAKDG
                     QDGAKDDADTLKDIALAYMRVVTFGKDVPGQPHVAGALLRVAQIEEKLKEPQVALALY
                     QQITKDYGDQPAAADARAG"
BASE COUNT          480 a          743 c          720 g          388 t
ORIGIN      
        1 gatgtcggtc cggtgagcac gtcgcccctc atcgtcaacg tcagcggtgg caccgctgcc
       61 ttcgcctcaa cccagcacct gtccgccgtc aacgtgtcgg gcgccggcct cgcccagatg
      121 tccgccgccg ccacccccgc ggcagccaat gtgctgaacg tcacgtcgat cagcttcacc
      181 ggcgcgagtg ccaagattga cctgacgaac aacgagatga tcaccaccac gacgccggcg
      241 gtcatccgcg gcgacatcgc cgctcactcg ctcatcacgt ccatcaccac cccacttggc
      301 cgaaatttgg gttacaagga cctgaacggc acgcaaaccg aagtgcgata cacgttgctc
      361 ggtgacaccg acctgaatgg agcggtgaac gtgggcgacc tgggcgcgct ggcaaccaac
      421 tacggcacaa cggccggcgc cctctggtcg caaggtgatt tcaattacga cgcgaaagtt
      481 gatgtcgccg acctgggcgc gttggcgacg aactacaatg tcgctctcgg attcggcgct
      541 ggctctgcca cggtcgccat cgccgaaccc gcggcctcaa ccgccgcggc gattcccgaa
      601 ccggcgtcgg ttgttttgat cgcggcaggc gcactgggct tatgcctgcg ccgccaacgt
      661 cgcctacgtg tgcggccata gcgcacgacg acaggtgatg gcttggcacg atgacacccg
      721 cgctgtcagc tcaatcggct ggtagtcctt gggatcaggt ttcaactgct tcggtcaacc
      781 cgatatcagc ctgacggcgt cgggcgcgca tcatcaacca aatcttccgc ccgatgaaca
      841 gtgcgatctg gctcaggaga gctgcaccga agccggacag gcggaaggcg ccgagccata
      901 gggcagcgcc gagcatagcg aagatgatgc cgccatacat ccagtatccc gacaggatga
      961 tgatcggcac gaagagatat cgcaccccgc cggcgtattg ttcgacgtcc gacggcttcc
     1021 cgtaaatata gccgaggatc gggattgtca cgatcaggtg aacccagcga aggatcgacc
     1081 gcttggtggc gttggtcatg gtgatgttcc taacgatttt gcctgcaaag gcaacgtgag
     1141 tcctgacaag ctccggcgtc gcgcccccga cagtggacgc gtcagcctac ccaccgaacc
     1201 cggcgtggta gatcgtggcg aatcgcttcg attgttccgc ccccccgcgt gcgttacaat
     1261 gtctcttctc gatgcatttg cgcacaaagc atctcacgct cgccacgctg ctggcggcct
     1321 tggcggccgc gtcgggtgtt gcgcgcactg ctaacgcaga ctccatctgg atcggcgcga
     1381 ccaccggcac acccatcaag gccgacggca tcaaagtgct gcgcatcgaa ggcgattcgc
     1441 tcgcgtacac cacgcctagc ggttcgcaga cgtccaagcc gctcagccag attgcgcaga
     1501 tggcctccga cgatgaaccg gccttcaacg ccgccgagca ggcgtatcgt gacggcaagt
     1561 gggccgacgc cgtcgatggg tatcagaaag ccgtgcagac gaccgcgaag gattgggtgc
     1621 gcgatcgatc gagcatccgg ctggtcgatg tggccgccaa gaccaaccgc ttcgacgccg
     1681 cggtgacggc gtacgtcgcc ttggtgcaga aggacccaac gctggccgcg aaggcgaaac
     1741 cggcactgcc ggatgcgaat tcgaaatatc tcgacggggc tatcgccgac atcaacaagg
     1801 cgctggaatc cacgcgattg agcgacgcgc agcgaagtag tcttctcagt ttcgaactgg
     1861 acatttaccg agcccgcaaa gacaccgcca acgtgaacca gacgttgcaa cagctggtga
     1921 agctgggcgc tgccacgccg gcggatttgg cgatgctgaa gctggcatcg gcgcatgcag
     1981 cgctcgacgc caaggagtac tccaaagcaa tcggcgaaat cgagcagaat cgcaagctct
     2041 tcatggatcc ggccaaccag ctggatgcgc tgcagacgct ggcgcaggcg aaagatggac
     2101 aggatggcgc caaggacgac gcggatacgc tcaaggacat cgcgctggcg tacatgcggg
     2161 tggtgacgtt cgggaaagac gtgccgggcc agcctcatgt tgcaggggcg ctgcttcgcg
     2221 tggcgcagat cgaggaaaaa ctgaaggagc cgcaggtggc gctggcgctg tatcagcaga
     2281 tcaccaagga ctacggcgat caacccgccg cggctgatgc gcgtgcgggc g
//
LOCUS       DATIMH010000293         3774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5553242, whole genome shotgun sequence.
ACCESSION   DATIMH010000293 DATIMH010000000
VERSION     DATIMH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5553242"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VLI90_04980"
     CDS             <1..186
                     /locus_tag="VLI90_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13590.1"
                     /translation="HDLTKLPLPVIELAAVDTRIEGLAHMAPFCPAALEQTANYLFVS
                     VRPDGSFELKAERPRRS"
     gene            complement(203..2305)
                     /locus_tag="VLI90_04985"
     CDS             complement(203..2305)
                     /locus_tag="VLI90_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV13591.1"
                     /translation="MPKTLQNNVLTLADDNPFHAPDPYRLAPRRRRFVLQIFLLVAIG
                     FAVFWPALAAGPIWQDVGKITTNTALRTWAGVGRLWVHPNAQAVYAPLTSTLLGAEHR
                     LWVAELMRYRVVSLLLHVTSVLLIWKILRRLEAPGAWLAAALFAVHPVDVQAVVWVSR
                     QGLILSTTLSAGALLAYLRWRDVAPPPPEDQLWEPPRWRGWLYLLAIVLFVAAVLAHP
                     VAGVMPVVLLLVLWWRDRLPRNELLWLSPVLLIAIATFMLALMTPHAELPLSPLGISL
                     MWGRAVVFSALKIICPYPLHFVYARWEIRATDPLAYLWLAGAVASPICAWLLRPRIGR
                     GVFVGVVSYELALSAGLWLGAREWSTYSYIADFFQYPASIALIALLAAGVVGLGRRVS
                     SPEGARLARLALGGSLMIAAGVLTWRQASSYASELTLWQSELAKGGAPPIIHDRLGKL
                     LLDQQNVEEAAAQFNAALARDPADVEANIGLGRCFEAESRLDDAVQQYRKAIALSPNQ
                     ASLYTTLAGVLVRQHHPDEAIDAYQRAIATEPADESAHNNLGLLLADSGKLAAAVTQY
                     KAALQSNPDSTAVRLNLASAYFREGDYDRAAEQLQEVVRIDPVNFEAFMNSGAMLGQL
                     REFPKAEEMFRAAVRLRPESPEAYNNLGLALAAQGKFNDATYNFAKAVQLKPDYAEAK
                     ENLAKAKQRLPRTTTEAL"
     gene            2747..>3774
                     /locus_tag="VLI90_04990"
     CDS             2747..>3774
                     /locus_tag="VLI90_04990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV13592.1"
                     /translation="MRPLRICHLGKYYPPAPGGIETHVRTLATAQAALGADVRILCVN
                     HTDTRGRDVTWSRYGATETVTETDHGVRVTRLGRSAHVAKLDVVPELRKHFRNFKRDP
                     VDVLHLHTPNPTMLLAVAALRPWAPLVITHHSDVVKQRKLYLAFAPFEQMVYERATRI
                     ISNSPNYIDGSDILQAHREKVQSLAMGLDLQEFQHPSAAALKHAAELRRRFAGGPLWL
                     SVGRCVYYKGYDTALRALRSVPGNLIIIGQGPYQVGLIEMARALGVADRVHWWGYATR
                     DELVGSYLAASAFWFPSNARSEAFGLVQVEAMASGCPVINTRIPASGVAWVSPHERTG
                     LTVPVGDA"
BASE COUNT          715 a         1258 c         1148 g          653 t
ORIGIN      
        1 catgacctga caaaactgcc cttgcccgtg attgaactgg ccgcggtgga cacacgaatc
       61 gaaggacttg cgcatatggc gccattctgc cccgcggcgc tggagcagac ggcgaactac
      121 ttattcgtaa gtgtccggcc cgacgggtcg ttcgagttaa aggcggagcg accaaggcga
      181 tcgtgaatgc aatcgctcgc ttttacagcg cctccgtcgt tgtcctaggc agccgctgct
      241 tcgccttcgc caggttctcc ttcgcctccg cgtaatccgg tttcaactgc accgctttgg
      301 cgaagttgta cgtggcgtcg ttgaacttgc cttgggctgc cagcgctaag ccgaggttgt
      361 tgtacgcctc gggtgattcc gggcgcaggc gcaccgcggc gcggaacatt tcttcggctt
      421 tggggaactc gcgcagctgg ccaagcatcg cgccggaatt catgaacgcc tcgaagttca
      481 ccggatcgat tcgcacgacc tcctgcaatt gctcggcggc gcggtcgtaa tcgccttcgc
      541 ggaagtaggc gctggccaag ttcagtcgca ctgccgtcga gtccgggttc gactgcagcg
      601 ccgccttgta ctgcgtcacc gcggcggcga gctttcccga atcggccagc agcaggccga
      661 ggttgttgtg cgccgattca tccgccggct ccgtggcgat cgcgcgctga tatgcgtcga
      721 tcgcctcgtc ggggtggtgt tgtcgaacca agacgccggc cagcgtcgtg tagagcgacg
      781 cttgatttgg cgacagcgcg atcgccttgc gatactgctg gactgcgtca tccagtcgcg
      841 actcggcctc gaaacaccgc ccgaggccga tgtttgcttc gacatcagcc gggtcgcgcg
      901 ccaacgccgc gttaaactgc gccgccgcct cttccacgtt ctgctgatcg agcagcagct
      961 tgcccagccg atcgtgaatg atcgggggcg ctccgccctt ggccaattcg ctttgccaga
     1021 gcgtcagttc actcgcataa gacgacgctt gccgccaggt cagcacgccc gcggcaatca
     1081 tcaagcttcc acccagcgcc aatcgtgcca accgcgcgcc ctccggcgat gacacgcgcc
     1141 ggccaagccc gacgacgccc gctgccagca gggcgatcag cgcgatcgac gccggatatt
     1201 ggaagaaatc cgcgatgtat gaataggttg accattcgcg tgcaccgagc cacaggccgg
     1261 cgcttagtgc gagttcgtag gagacaacgc caacgaacac gccgcgaccg atgcgcgggc
     1321 gaagaagcca cgcgcagatc ggcgacgcca ctgctccggc tagccataga tacgccagcg
     1381 gatccgtcgc gcggatctcc cagcgggcat agacgaagtg cagcggatac gggcaaatga
     1441 tcttcaacgc ggagaaaacc accgcccggc cccacatcag cgaaatgccg agcggcgaca
     1501 gcggcagctc cgcgtgtggc gtcatgagtg ccagcatgaa cgtagcaatc gcaatcaaca
     1561 gcaccggcga cagccaaagc agctcgtttc gcggcagccg atctcgccac cacaggacca
     1621 gaagtaagac gaccggcatc acccccgcca ccggatgcgc cagcaccgcc gcaacgaata
     1681 ggacgatcgc cagcaaatac aaccacccgc gccagcgcgg cggttcccac agctgatcct
     1741 ccggcggcgg tggcgcgacg tcgcgccatc gcaagtacgc gagcagcgcc cccgccgaca
     1801 gtgtcgttga taagatcagt ccctgacggc tcacccacac gaccgcctgc acgtccaccg
     1861 gatgcaccgc gaacagcgcc gccgccagcc atgcgcccgg cgcttccaat cgtcgcagga
     1921 ttttccagat cagcagcacg ctggtcacgt gcaggagcag gctgacgacg cggtagcgca
     1981 tcagctccgc cacccagagc cggtgctcgg cgcccaggag cgtgctcgtc agcggcgcat
     2041 agaccgcctg cgcgttcgga tgcacccaaa gccggccgac gcccgcccag gtgcgcaggg
     2101 cggtgttggt ggtgatcttc ccgacgtcct gccagatcgg ccccgccgcc agcgcgggcc
     2161 agaagacggc aaagccaatc gccacgagca agaagatttg cagcacaaac cgccgccgcc
     2221 gcggggcaag ccggtagggg tccggcgcgt gaaacggatt gtcgtcagcg agggttaaca
     2281 cgttgttctg caatgtcttc ggcatcgcct ggtggtcaga ttgatcgaaa tcagcccctc
     2341 agcattaggc cccatttccg gttgaaggcg cgccccaaac gaaccactcc cccaaacccg
     2401 ccgtaccaac gcccgcagac tctaccatgc cgcccggccg gtgccgcgcg aacttgctta
     2461 tcgccgttaa ctcgttcagc aacaggcgga tatccggata ttggtctaat ccgcaaattc
     2521 cttgacttgc cgtgagcggt gatgcaaatt gagtttgtca ggctaagggc ttcgtctgcg
     2581 ggatttaccg tatgctcatg aagtgatcgt gaggaacagc gctgcaaggt cagtgcgtgc
     2641 tgttcgacgt ggatatgttt gcgtgaggtt aaagggttaa cccgggaaaa tggcatcggg
     2701 cgccacggac gtttgtgggc gttggggcgt ggagagggtg tggagcgtgc ggccgttacg
     2761 aatctgtcat cttggcaaat actatccgcc cgcgccgggt ggaattgaaa cgcacgtgcg
     2821 cacgctggcg accgcgcagg cggcactcgg cgcggacgtg cgcatcctct gcgtcaatca
     2881 caccgacacc cgcggccgcg atgtcacctg gagccgctac ggcgcgaccg aaaccgtcac
     2941 cgaaaccgat catggcgtgc gcgtcacgcg gctgggccgc agcgctcacg ttgccaagct
     3001 tgatgtcgtc cccgaacttc gcaaacactt tcgcaatttc aagcgcgatc cggtggacgt
     3061 gctgcacctg cacacgccca acccgacgat gctgctggcc gtcgccgcgc ttcgcccgtg
     3121 ggcaccgctg gtcatcacgc accacagcga cgtcgtcaag caacgcaagc tctatctcgc
     3181 ctttgcgccg ttcgagcaga tggtgtatga gcgcgcgacg cgcatcatct ccaacagccc
     3241 gaattacatc gacggctccg acatcctcca ggcccatcgc gaaaaagttc aatcgctggc
     3301 aatgggactg gacctgcagg aatttcaaca ccccagcgcc gcggcgctca aacacgcggc
     3361 cgagcttcgc cggcgattcg ccggcggacc gctgtggttg agcgttggtc gttgcgttta
     3421 ctacaaaggc tatgacaccg cccttcgcgc gctgcgatcg gttccgggca acctgatcat
     3481 catcggccag ggaccgtacc aggttggcct gatcgagatg gcccgcgccc tgggcgtggc
     3541 cgaccgcgtg cactggtggg gctatgcgac gcgcgatgaa ttggtcggtt catacctcgc
     3601 cgccagcgca ttctggttcc cgtccaacgc ccgcagcgaa gcgttcggcc tggtgcaggt
     3661 cgaagcaatg gccagcggtt gcccggtcat caacacgcgc atccccgcca gcggtgtggc
     3721 gtgggtcagc ccgcacgagc gcaccggatt gaccgttcca gtcggcgacg ccca
//
LOCUS       DATIMH010000294         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4976300, whole genome shotgun sequence.
ACCESSION   DATIMH010000294 DATIMH010000000
VERSION     DATIMH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4976300"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1222
                     /gene="trxB"
                     /locus_tag="VLI90_04995"
     CDS             200..1222
                     /gene="trxB"
                     /locus_tag="VLI90_04995"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HSV13593.1"
                     /translation="MAAEKLVIIGSGPAGWTAAIYAARANLSPVVYEGALSEDNRLRG
                     TLPLGQLNLTTEVENFPGFAKGIMGPDLMLAMREQAERVGTRIITEDIVDIDLSKRPF
                     TMKDSADGTLTAEAVIIATGASANYLGLESESRFKNHGVSACAVCDGALPRFRNKPLI
                     VVGGGDSACEEGTYLTKFASTVHLVYRKSREQMRASPIMAERILSHPKVKPIWNSVID
                     EVHGDDERGMTGVRVKNLSTGETQAIDAPGMFVAIGHTPNTKFLKGQLKTDAQGFLVL
                     RDAARTTTSVDGVFAAGDVADPIYKQAITAAGMGCKAALDAERWLEAQHTHNPNTPMK
                     SQWNAG"
     gene            1262..1669
                     /gene="rnpA"
                     /locus_tag="VLI90_05000"
     CDS             1262..1669
                     /gene="rnpA"
                     /locus_tag="VLI90_05000"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HSV13594.1"
                     /translation="MGWWRPCYNKYVAERTHTYPKSRRLGGRLQFAAVFDAKAKESRG
                     PLMLYSLPNDLGHPRLGISISRRVGNAVRRNRIKRLLREAFRAHQHDLPDGYDLVFVV
                     RPHAPLILAEYQKLMTALMLKAHATWQRRKPLA"
     gene            1761..1967
                     /gene="yidD"
                     /locus_tag="VLI90_05005"
     CDS             1761..1967
                     /gene="yidD"
                     /locus_tag="VLI90_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HSV13595.1"
                     /translation="MSWLLIQLVLLYRVTLGRLLGGQCRFTPTCSQYAIDAITKHGPL
                     RGSWMAIKRICRCHPWGGQGYDPA"
     gene            complement(1994..>2769)
                     /locus_tag="VLI90_05010"
     CDS             complement(1994..>2769)
                     /locus_tag="VLI90_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSV13596.1"
                     /translation="VTSLEELRLALHRLDTPAVLKTAGWGYDGKGQITLKTADHADAA
                     WSGLETDEAVLEAFVDFEKEVSVVAARGADGSFAHWGVIENNHQHHILDTSLAPAAVP
                     PRTAQRAIEIARGVLEQLNVVGVLCVEFFLRKDGELVINELAPRPHNSGHLTFDASVT
                     SQFEQQARAVTGMSLGSTQLLRPAAMANLLGDLWFGDGNHVEPNWPAALASSPDVKLH
                     LYGKSDARPGRKMGHITAMAETPEAALERVVAAREALKA"
BASE COUNT          516 a          959 c          849 g          445 t
ORIGIN      
        1 agggttaggg tgagggtgat ttggaatcac tagcggccat cgcggcagaa atcaccctca
       61 ccctaaccct ctcccatgag tacatgggcg aggggaccag aaaaggcgtt cgccacaatc
      121 acgagttaca cccttcgaac ccggcgcggt ccctccccat tcgtctccac cgcgtcctcg
      181 gctacaatac gccagacaca tggcagcaga gaaactcgtc atcatcggtt ccggccccgc
      241 cggatggacc gccgcgattt atgcggcccg cgccaacttg agccccgtcg tctatgaagg
      301 cgcgctcagc gaagacaacc gcctccgcgg caccctgccg ctggggcaat tgaacctgac
      361 gaccgaagtc gaaaatttcc ccggcttcgc caagggcatc atgggccccg acctgatgct
      421 ggcgatgcgc gagcaggccg agcgcgtcgg cacgcgcatc atcaccgagg acatcgtcga
      481 tatcgacctg agcaaacggc cgttcacgat gaaggactcc gccgacggca cgctgaccgc
      541 cgaagcggtc atcatcgcga cgggggccag cgcaaattac ctcgggctgg aatcggaaag
      601 tcgcttcaag aaccacggcg tgtccgcgtg cgccgtctgc gacggcgcgc tgccgcgctt
      661 tcgcaacaag ccgctcatcg tggtcggcgg gggtgactcg gcgtgcgaag agggcacgta
      721 cctcaccaag ttcgccagca ccgtgcacct ggtctaccgc aagtcgcgcg agcagatgcg
      781 ggccagcccg atcatggccg agcgcatcct ctcgcacccc aaggtcaagc cgatctggaa
      841 cagcgtgatc gacgaagtcc acggcgacga cgaacgcggc atgaccggtg tgcgcgtgaa
      901 gaacctgagc accggcgaaa cccaagccat cgacgctcct ggcatgttcg tggcaatcgg
      961 tcacacgccg aacacgaagt tcctcaaggg ccagctgaag accgacgccc aaggcttcct
     1021 cgtcctgcgc gatgcagcgc gcacgacgac cagcgtagat ggcgtcttcg ccgccggcga
     1081 tgtggccgat ccgatctata agcaagcgat caccgccgcc ggcatgggtt gcaaggcggc
     1141 gctggacgcc gagcgctggc tcgaagcgca gcacacgcac aacccgaata cgccgatgaa
     1201 atcgcagtgg aacgcggggt aaagtagcac ggacgccaga gcccgttccg gacacctcgg
     1261 catgggctgg tggcgtccgt gctacaacaa atacgtggcc gagcgcacgc acacctaccc
     1321 gaaatcgcgc cgcctcggcg ggcggttgca gtttgccgcc gtcttcgacg ccaaggcgaa
     1381 ggaatcgcgc ggcccgctga tgctctactc gctgcccaac gacctgggcc acccgcggct
     1441 cggcatcagc atctcccgcc gcgtcggcaa cgcggtgcgg cgcaaccgca tcaagcggct
     1501 gctgcgcgag gcgtttcgag cgcatcagca cgacctgccg gatgggtatg atctggtgtt
     1561 cgtcgtgcgg ccgcacgcgc cgctgatcct ggcggaatat cagaagctga tgaccgcgct
     1621 gatgctgaaa gcgcacgcga cgtggcaacg gcgcaaaccc cttgcgtagc ggttgcctcg
     1681 caggggcacg tgtttctttt gcgaacgaaa aacgcgtgcc ctgcgggcaa ccgctacgcg
     1741 tagagatatc tttacaggat atgtcctggc tcctcatcca actcgtcctg ctctaccgcg
     1801 tcacgctcgg gcgcctgctc ggcgggcagt gtcgcttcac gcccacgtgc agccaatacg
     1861 cgatcgacgc gatcaccaag cacggcccct tgcggggttc ctggatggca ataaaaagaa
     1921 tctgccgctg ccacccctgg ggcgggcagg gctacgaccc ggcgtgaggt gactggagcc
     1981 gcaagcggcg acttcacgcc ttcagcgcct cgcgcgctgc gacgacgcgc tccagcgccg
     2041 cctccggcgt ctccgccatc gcggtgatgt ggcccatctt ccgccccggt cgcgcgtccg
     2101 acttcccgta gagatgcagc ttcacatccg gcgacgacgc cagcgccgcc ggccagtttg
     2161 gctcgacatg gttgccatcg ccgaaccaaa ggtcgcccag caaattcgcc atcgccgccg
     2221 ggcgcagcaa ttgcgtcgat ccgagcgaca tgccagtcac cgcgcgggct tgctgctcga
     2281 attggctcgt cacgctcgcg tcgaacgtca ggtgtcctga attgtgcggc cgcggggcca
     2341 gttcgttgat gaccaattcg ccatccttgc gcaggaagaa ctcgacgcac agcacgccga
     2401 cgacgttcag ttgctccagc acgccgcggg cgatttcgat cgcgcgctgc gccgtccgcg
     2461 gcggcaccgc cgcgggggcc agcgaggtgt cgaggatgtg atgctgatgg ttgttctcga
     2521 tcacgcccca atgcgcgaat gacccatccg ccccgcgggc ggcgacgacg gagacttcct
     2581 tctcgaaatc cacgaacgct tcgagcaccg cttcgtcggt ctcgaggccc gaccatgccg
     2641 cgtcggcgtg atcggcggtc ttcagcgtga tctgcccctt cccgtcgtaa ccccacccgg
     2701 ccgtcttcag caccgccggg gtgtccaggc gatgcagcgc cagccgcaac tcttccagcg
     2761 atgtcaccg
//
LOCUS       DATIMH010000295         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3532235, whole genome shotgun sequence.
ACCESSION   DATIMH010000295 DATIMH010000000
VERSION     DATIMH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3532235"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VLI90_05015"
     CDS             complement(<1..1166)
                     /locus_tag="VLI90_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13597.1"
                     /translation="MSTAAPSRHVAPDSARRRFWVTVLCFALANLAAWVVYDRVIAYR
                     HRGTLRVDAFEPGDGAVVGARPNVRFHFSADVIPTALYGHDPARITPSIPGRWTWDDP
                     RTLVFTPAADLPRATRVHFALDQQFIRSGTGATLNGPYVAAVDAQPLELLDARQTALE
                     DRDQYILELRFNDRVAPGDVLQHLTMHTAAGRTVTGQLHGEAAGNIVRIRTESVEPDR
                     PDGDTELHLHLAPGVVGLSGPLGIDRDIDQTVNLARALAATDLKAYVPARGQPYLLLS
                     FNNEIDSLALKEIIAIDPATPFTIESDGGQTVRLLGDFKPGTRYTLKLNAAPAGADRA
                     KYPRAAQLAAFMPDRGSGVWFDNDQGYLSTAGNRALLVHAVNLDALRVSVTRMY"
     gene            complement(1387..2532)
                     /locus_tag="VLI90_05020"
     CDS             complement(1387..2532)
                     /locus_tag="VLI90_05020"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HSV13598.1"
                     /translation="MRRLTDEPNSVGFYFNINAYTPDGREMVYSTPDSIRVLNLETMQ
                     TRPVVSGANLKTVVVGRKTPSIFFIKQDERELCVADINTGAVRTLAKVPARGLIASVN
                     AAETLAAGTYIEGSAGEDYGEKRVMPPGMSGPLVQPLNKARMMEERLAAHLPLVLFTI
                     DLKTGQMRELLHSTDWINHLLFSPVDPSLLMYCHEGPWHKVDRIWTIRTDGSQNQLIH
                     KRTMNMEIAGHEFWGQAGETIWYDLQTPKGEDFWLAAYDLPTSRRRWFHLQRNEWSIH
                     FNVTSDESLFCGDGGDTGQVARAPDGKWIELFRPQSLDGQDPAPDLIHPGVFHSEHLV
                     NMSKHNYRLEPNVRFSPDDKMVLFSSNMFGPSYVFGVDVQKAEQAGK"
BASE COUNT          472 a          829 c          971 g          487 t
ORIGIN      
        1 tcgtacatgc gtgtgacgct gacgcgcagc gcgtcgaggt tgacggcgtg cacgagcagc
       61 gcgcggttgc cggcagtgga gagatagccc tggtcgttgt cgaaccacac gccgctgccg
      121 cggtcgggca tgaaggcggc cagctgggcg gcgcgcggat acttggcgcg gtcggcgccg
      181 gcgggggcgg cgttgagctt cagcgtgtag cgcgtgccgg gtttgaagtc gccgagcaat
      241 cgcacggttt ggccgccgtc gctttcgatc gtgaacggcg tggcgggatc gatcgcgatg
      301 atttccttca gcgccagcga atcgatttcg ttgttgaagc tgagcagcag gtagggctgg
      361 ccgcgcgccg ggacgtaggc tttgagatcg gtggcggcca gcgcgcgggc gaggttgacg
      421 gtctggtcga tgtcgcgatc gatgccgagc ggaccggaga gaccgacgac gccgggcgcg
      481 aggtgcaggt gcaactccgt gtcgccgtcg ggacgatcgg gttcgacgga ttcggtgcgg
      541 atgcgcacga tgttgccggc ggcctcgccg tgcaattggc cggtgacggt gcggccggcg
      601 gcggtgtgca tcgtcaggtg ctgcaacacg tcgccggggg cgacgcggtc gttgaagcgc
      661 agctcgagga tgtactggtc gcggtcttcc agggcggtct gccgggcgtc gagcagttcc
      721 agcggctggg catcgaccgc ggcaacgtaa ggaccgttca acgtcgcgcc ggtgccgctg
      781 cggatgaact gctggtcgag cgcgaagtgc acgcgcgtcg cccgcgggag gtcggcggcc
      841 ggcgtgaaga cgagcgtgcg cggatcgtcc cacgtccatc gcccggggat gctcggcgtg
      901 atgcgcgccg ggtcgtggcc gtagagcgcg gtggggatga cgtcggcgct gaagtggaag
      961 cgcacgttcg gccgagcgcc gacgaccgcg ccgtcgccgg gctcgaaggc gtcaacgcgc
     1021 agcgtgccgc ggtggcgata agcgatgacg cggtcgtaga cgacccacgc ggcgaggttg
     1081 gccagcgcga agcagagaac ggtcacccag aatcggcggc gggcggaatc gggcgcaaca
     1141 tgacgcgaag gggcggcggt cgacatagcc agtcctccat gactcggcga tcgagcgtgt
     1201 cagcgagagt cgcagattgt gacgcgtcgg cagaaattcg caatagaatt ttgcggcgtg
     1261 ccgggaagcg aaggaaaggt gttcgtagca cgggcatctc gcccgtgcca aggtgctcgg
     1321 caccggcgag acgcccgtgc tacagcaggc aaaaatcaag cgccgcaccg tgcgttgatg
     1381 aagcggctac ttcccggcct gctccgcttt ctgcacatcg acgccgaaca cataacttgg
     1441 cccgaacatg ttcgagctga ataacaccat cttgtcgtca ggactgaacc gcacgttcgg
     1501 ctcgagcctg tagttgtgct tcgacatgtt gaccaggtgt tccgagtgga acacgccggg
     1561 gtggatcaga tcgggcgcgg gatcctggcc atccagcgac tgcgggcgga acagctcgat
     1621 ccacttgccg tcgggcgcgc gggccacctg ccccgtgtca ccgccgtcgc cgcagaacag
     1681 gctttcgtcg ctggtcacgt tgaagtggat cgaccattcg ttgcgctgca ggtggaacca
     1741 gcggcggcgc gacgtcggca ggtcgtacgc ggccagccag aaatcttcgc ccttgggcgt
     1801 ttgcaggtcg taccagatcg tctcgccggc ctggccccag aactcgtggc cggcaatctc
     1861 catgttcatc gtgcgcttgt ggatcagctg attttgcgag ccatccgtgc ggatcgtcca
     1921 gatgcgatcg accttgtgcc acgggccctc gtggcaatac atcagcagcg atggatcgac
     1981 cggcgaaaac agcaggtgat tgatccaatc ggtgctgtgc agcagctcgc gcatctgccc
     2041 cgttttcaaa tcgatcgtga acaacaccag cggcagatgc gcggccaggc gctcttccat
     2101 catccgcgct ttgttcaacg gctgcaccag tggcccggac atgcccggtg gcatcacgcg
     2161 cttctcgcca taatcttcac cggccgagcc ttcgatgtac gtgccggccg cgagcgtttc
     2221 ggcggcgttg acgctggcga tcaacccacg cgccggcacc ttcgccagcg tgcgcaccgc
     2281 gccggtgttg atgtcggcca cgcacagctc gcgctcatcc tgcttgatga agaaaatgct
     2341 cggcgtcttc cggccgacga cgaccgtctt cagattggcg cccgacacga ctggccgcgt
     2401 ttgcatcgtc tcgaggttca acacgcgaat gctgtcgggc gtggagtaaa ccatctcgcg
     2461 cccatcgggc gtgtaggcgt tgatgttgaa gtagaagccg acggagttgg gttcatcggt
     2521 gagacgacgc acgcgatgat ccgtgtctgg gtcgatccaa tctttcggcg gcggttcaga
     2581 cggcgcggtc gtttgagcgc gcgccagcgc cggcaaaaga caagccgagc caatgaccac
     2641 gaaacgtgcg attgaattga gcatgcgtcg cctgcgcaga aaggtcatgt cacacaccac
     2701 gccgggttcg gaggtactcc gacgacccgg atccttccgt gggggtggga aaagcatcc
//
LOCUS       DATIMH010000296         7420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_647132, whole genome shotgun sequence.
ACCESSION   DATIMH010000296 DATIMH010000000
VERSION     DATIMH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647132"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="VLI90_05025"
     CDS             <1..1286
                     /locus_tag="VLI90_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HSV13599.1"
                     /translation="LLSWYLFMIGGLLAFVAMVAGGVDTGWTFYAPYSTTYSNSYVSL
                     TLTGIFINGFSSILTGLNFIVTIHKMRAPGLTWFRLPLFLWSTYATSVIFILGTPVVA
                     ISLLLIVFERTFHIGIFDPAIGGDPVLFQHMFWFYSHPAVYIMVLPAMGVVSEIIACF
                     SRKRIFGYEMVAFSSLAIAIIGFLVWGHHMFVSSQSAYAGMIFSVMSFLVAIPSAIKV
                     FNWTATMYKGSIELRTPMLYALGFIGLFTLGGLTGLFLASMGMDVHLHDTYFIIAHFH
                     YIMVGGAVMGFMGAVHFWWPKMTGRMYPEWWGRFSAFTIFMGFNLTFFPQFILGYLGM
                     PRRYHSYPDEFQVLNVMSTAGASILAIGYALPLCYLLWSLKWGEVAGNNPFKATGLEW
                     QTESPPITENFETTPIVTQEPYDYEHMEPQGVGTV"
     gene            1270..2058
                     /locus_tag="VLI90_05030"
     CDS             1270..2058
                     /locus_tag="VLI90_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HSV13600.1"
                     /translation="MSEPSNAISPAAGTNPLLPSMLGEEAPHGDSHGHHGLVAHHFAD
                     RAQQLESGTLGMWLFLSTEVMFIGAIFVAFFAYRLNPYYFRAFRLGSENLITWIGAVN
                     TAVLLTSSLTVVLAIRAAQLGKQNQIVLNLILTIILGSMFFGFKVLEYYKDYQEHLWP
                     AVGAVFEPEKGFDVQKDFGGDVAAHDLTVARSKLFFRFYYSLTGLHALHMLVGLGIFV
                     YLLVRAIRGRYTPQSHAQLEISGLYWHFVDLVWIFLFPLLYLIR"
     gene            2097..2501
                     /locus_tag="VLI90_05035"
     CDS             2097..2501
                     /locus_tag="VLI90_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HSV13601.1"
                     /translation="MAHRHKDYDEHHEEVDPLRAYILVFVALMVLLALTLVAYEIPFE
                     KIPPKGTFDFMNTAIALTIATIKALLVMLVFMHLRHSTRLTWVIASAGFIWLFIMISF
                     TFSDYLSRHAIIEGVKEPHTPPGMSMPHEPEH"
     gene            2661..3650
                     /locus_tag="VLI90_05040"
     CDS             2661..3650
                     /locus_tag="VLI90_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV13602.1"
                     /translation="MRLGFIGGYGHHYLRGALTDPACDITEIAVAGDGHDDARAETFA
                     RSLPNNPRWFPDAAAMIDQFRPHAISVGTIYGFNGDLIAQSLEANIPTVSDKPIAATW
                     PQFHRIQELSKQDPRRVICTEFDFRCRPDFCAAKKAVDDGAIGDICLAVAQKSYRWGT
                     RPDWYKDRRSYGGTLLWIASHGVDVIPFTTGRHYKKVIGFHGNVTHPEFGEKFDDHCV
                     AIYKLDNGGSAAVHADFSRPVKAATHGDDRLRLSGSKGVIEVRGERCLLTTNEQPERD
                     ITDWGATRKIHVELLAALEGQSEKYGTASSLEMAEILLRSRDAIDETKLIDLS"
     gene            3763..4287
                     /locus_tag="VLI90_05045"
     CDS             3763..4287
                     /locus_tag="VLI90_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSV13603.1"
                     /translation="MFFLRDLPKYDAIRARAARYPEVDPAALESFLTLLRVGSDVLAA
                     WEKYLAGHHISQGRFTVLMVLNRDPITGLNPSDLAARCGVTRATMTGLLDSLERKRLI
                     RRESDQADRRTVLVRLNDKGIDLLEGMLHDYYRRISLLMGELSEDEKRSLTAMLTKVA
                     GSIRHVSPSESPSV"
     gene            complement(4308..5900)
                     /locus_tag="VLI90_05050"
     CDS             complement(4308..5900)
                     /locus_tag="VLI90_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13604.1"
                     /translation="MNRRRCRTAALAGAMFPLTFVGSTRAAAPAWSGYANDPFHAANA
                     PAASDPLSSIRWQTPVDLSPQFSGNDLLIHYGSPTITAANTLLVPVKTGASGGFEINA
                     INGATGSSKWSVTSDYVLPPHNWVPSYSPALTPNNRMYYAGAGGTIYWRDTLDGAAPS
                     AAHQLAFFGDANYAANPSAYNSNVFINTPITSDASGNIYFGFRVTGSTPLGAGLSSGI
                     ARIGADGSETFTSAAAAAGGDAVINNDLMNCAPALSRDGSKLYITLTGGGVGDLVSLN
                     SSTLAPQAHVRLKDPASGADAEVTDDGSASPTVGPDGHVYMGVLENPFPSNHDRGWML
                     HYTGDLSAQKTTGAFGWDDTASIVPASMVPGYAGPSSYLLMTKYNNYAGLGGDGVNKL
                     AILDPNATETDPVTGATVMKEVLSIAGVTPDGEFPSKPGAVREWCINSAVVDPSTDSI
                     LANSEDGKLYRWDLGTNTFMQVITLTPGVGEAYTPTLVGPDGAVYAINNATLFSVVPE
                     PTSGAMVIASVTLVLARRRRHV"
     gene            6173..7066
                     /locus_tag="VLI90_05055"
     CDS             6173..7066
                     /locus_tag="VLI90_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HSV13605.1"
                     /translation="MPGEFDFIEWIRSQQRPSHLVNTGVGDDLAVLKWPKDDLLIAGV
                     DQVLDGVHFDSKMHPPRAIGRKVMNRNLSDCAAMASLPAAAVATVALPRGVGHDYAKQ
                     LFLGMKEAGEVFDCAIVGGDTGSWDGKLVMTVTILGRSAGVQPVLRSGAKAGDGIYVS
                     GPLGGSILGRHMTFSPRVDLGRELAKRGDAAAMIDLSDGLSRDLRHICRLSGNLGAVV
                     YADRVPIHDDAQAAFRLDHVSPLDHALNDGEDYELLYTCRNVATMGIYIGDISSAPGI
                     WLQHADGKREPLEPKAWEHLL"
     gene            7132..>7420
                     /locus_tag="VLI90_05060"
     CDS             7132..>7420
                     /locus_tag="VLI90_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13606.1"
                     /translation="MLPYAITIFLGAFLLFQVQPLIGKYILPWFGGATAVWTTCMLFF
                     QLLLLGGYAYAHLSTQLLRPRAQSMLHVALLVAALCVLPIVPSPRWKPTPAD"
BASE COUNT         1331 a         2497 c         2151 g         1441 t
ORIGIN      
        1 acttgctcag ctggtacctg ttcatgatcg gcgggctgct ggcgttcgtc gcgatggtcg
       61 ccggcggggt cgataccggg tggacgttct acgccccgta ttcaacgacg tattccaata
      121 gctacgtcag cctcaccctc accggcatct ttatcaacgg cttcagctcg atcctcacgg
      181 ggctgaactt catcgtcacg atccataaga tgcgcgcccc aggccttacc tggttccgcc
      241 tgccgctgtt cctgtggtcg acttatgcaa ccagcgtgat ctttattctc ggcacgccgg
      301 tcgtcgccat ctcgctgctg ctgatcgtct tcgagcgtac cttccacatc ggcatcttcg
      361 atccggccat cggtggggac cccgtgctgt tccagcacat gttctggttc tacagtcacc
      421 cggcggtgta catcatggtc ctgccggcca tgggcgtggt cagcgagatc atcgcctgct
      481 tcagccgcaa acgcatcttc ggttatgaaa tggtcgcctt ctccagtctg gcaatcgcga
      541 tcatcggctt cctggtctgg ggacatcaca tgttcgtctc cagccaaagc gcctacgccg
      601 ggatgatctt ctcggttatg agcttcctcg tcgccatccc atcggccatc aaggtcttta
      661 actggaccgc gacgatgtac aagggctcga tcgagctgcg cacgccgatg ctgtacgcac
      721 ttggattcat cgggctgttc acgctcggcg gtctgaccgg tttgttcctg gccagcatgg
      781 gcatggacgt gcacctgcac gacacctact tcatcatcgc ccacttccat tacatcatgg
      841 tcggcggcgc ggtgatgggc tttatgggcg cggtgcactt ctggtggccc aaaatgaccg
      901 gccggatgta ccccgaatgg tggggccgct tcagcgcctt caccatcttc atgggcttca
      961 acctgacgtt cttcccgcag ttcatcctgg gttacctggg catgccccgg cgatatcact
     1021 cgtacccgga cgaattccag gtgctgaacg tgatgagcac cgccggcgcg tcgatcctgg
     1081 cgatcggtta cgccctgccg ctgtgttacc tgctctggtc gctcaagtgg ggcgaggtcg
     1141 cgggcaacaa cccgttcaag gcgaccggtt tggaatggca gaccgaaagc ccgccgatca
     1201 cggaaaattt cgagacgacg ccgatcgtca cgcaggaacc ctacgactac gagcacatgg
     1261 agccgcaagg tgtcggaacc gtctaacgca atttcccccg cggcgggcac gaacccgctg
     1321 ttgccgagta tgctcggcga agaagcgccg cacggcgatt cccacggcca tcatggcttg
     1381 gtcgcgcatc actttgccga tcgcgcccag cagctggaat ccggcacgct gggcatgtgg
     1441 ctgttcctgt ccaccgaagt tatgttcatc ggcgcgatct tcgtcgcctt cttcgcctat
     1501 cgcctgaacc cgtattactt ccgcgcgttc cggctcggca gcgaaaacct catcacctgg
     1561 atcggcgccg tcaacaccgc cgtgctgctg accagcagct tgacggtggt cctcgccatt
     1621 cgcgccgccc agctgggcaa gcaaaaccag atcgtcctga atctgatttt gacgatcatc
     1681 ctgggttcga tgttctttgg ctttaaggtc ctggaatatt acaaggatta tcaggaacac
     1741 ctctggcccg cagtgggggc ggtgttcgaa ccggaaaagg gattcgatgt tcaaaaggat
     1801 ttcggcggcg acgtcgccgc gcacgacctg accgtcgcgc gctccaagtt gttcttccgc
     1861 ttctactatt cgctgaccgg cctgcacgcc ttgcacatgc tggtgggctt gggcatcttc
     1921 gtctacctgc tggtgcgggc gatccgcggc agatacacgc cgcagagcca cgcgcagttg
     1981 gaaatcagcg gcctgtattg gcacttcgtg gacttggtgt ggattttcct cttcccgctc
     2041 ctgtacctga ttcgataacg tgcatgttcc tgaccccctg acacgcgact cctcccatgg
     2101 cccatcgaca caaagattac gacgagcacc acgaagaggt cgatccgctc cgcgcttaca
     2161 tcctggtgtt cgtcgcgctg atggtgctgc tggccctgac gctggtggca tacgaaatcc
     2221 cgttcgaaaa gatcccgccc aagggcacct tcgatttcat gaacaccgcc atcgcgctga
     2281 cgatcgccac catcaaggcg ctgctggtca tgctcgtgtt catgcacctg cggcatagca
     2341 cgcgcctgac ctgggtcatc gcctccgccg ggttcatctg gctgttcatc atgatcagct
     2401 tcacgttcag cgattacctc agccgccacg ccatcatcga aggggtcaag gaaccgcaca
     2461 cccccccggg gatgtccatg ccgcacgagc cggagcattg aggtttccct tgtcctgagc
     2521 gcagcacgta ctcgtgcgga gtcgaaggat atagcctcga gcgtcggtga acctctcgca
     2581 cgcgaaaggt tcaccgacgt tcgaggctat atccttcgac ttcggcggag taccgcctgc
     2641 gctcaggaca aggtgaaact atgcgactcg gcttcatcgg cggttacggt caccactacc
     2701 tgcgcggcgc cctcaccgat cccgcctgcg acatcaccga aatcgccgtc gccggcgatg
     2761 gacatgacga cgcccgcgct gaaaccttcg cgcgttccct gcccaacaac ccgcgctggt
     2821 tccccgacgc cgccgcgatg atcgaccagt tccgcccgca cgcgatcagc gtcggcacga
     2881 tctacggatt caacggcgac ctcatcgccc aatccctcga agccaacatc cccaccgtca
     2941 gcgacaaacc catcgccgcc acctggccgc agttccaccg catccaggaa ctctcgaagc
     3001 aggatccccg gcgggtgatc tgcaccgagt tcgacttccg ctgccgcccc gatttctgtg
     3061 cggccaaaaa agcggtggac gacggcgcga tcggtgacat ctgcctcgcc gtcgcccaga
     3121 aaagttaccg ctggggcacc cgccccgatt ggtacaaaga ccgccgcagc tacggcggca
     3181 cgctgctctg gatcgccagc cacggcgtcg atgtcatccc cttcaccacc ggccggcact
     3241 ataaaaaagt cattggcttc cacggcaacg tcacgcaccc cgagttcggc gaaaagttcg
     3301 acgaccactg cgtcgcgatc tacaagttag acaacggcgg ctccgccgcg gtgcatgccg
     3361 acttctcgcg ccccgtcaaa gccgcgaccc acggcgacga ccgcctgcgc ctcagcggct
     3421 ccaaaggggt catcgaagtc cgcggcgaac gctgcctgct gaccaccaac gaacagcccg
     3481 agcgcgacat caccgactgg ggcgcaacgc gaaagatcca cgtcgagttg ctcgccgcgc
     3541 tcgaaggcca atccgaaaag tacggaaccg cgtcctcgct ggagatggcc gagatcctgc
     3601 tgagatcgcg cgacgcgatc gacgagacga aactcatcga tctatcatga tccaggagtt
     3661 ctcgccgcgc gagccacttt ctcgctggcc gtttattgct accgcccgta acatgctcat
     3721 cgcacgcggc agcgagccgc ggatgttctg tcttttcgcc gcatgttctt cctccgcgac
     3781 ttaccgaagt acgacgccat ccgcgcccgc gctgcgcgat atcccgaagt cgatcccgcc
     3841 gccctcgaat cgtttctcac gctgctccgc gtcggcagcg acgtgcttgc cgcgtgggaa
     3901 aaatacctcg ccggccacca catctctcaa gggcgcttca ccgtgctgat ggtgctgaac
     3961 cgcgatccga tcacgggcct gaacccgtcc gacttagccg cccgctgcgg cgtcacgcgc
     4021 gcgacgatga ccggcctgct cgatagcctc gagcgcaaac gactcatccg ccgcgagagc
     4081 gaccaagccg accgtcgcac ggtgctggtc cggctaaacg ataaagggat cgatctgctg
     4141 gaaggaatgc tgcacgacta ttaccgccgc atctcgctgc tgatgggcga gctaagcgaa
     4201 gacgaaaaac gcagcctcac cgcaatgcta acaaaggtcg ccggcagcat ccgccacgtg
     4261 tcgccttctg aatcgcccag tgtttagcgg ccgcatttac gcggccgcta aacgtgacgg
     4321 cgccgccgcg ccagcaccag cgttacgctg gcaatcacca tcgcaccaga cgtcggctcc
     4381 ggcaccacgc tgaacagcgt cgcgttgttg atcgcataca ccgccccgtc cggtcccacc
     4441 aatgtcggtg tgtacgcctc gcccacgccc ggcgtcagcg tgatcacctg cataaaggtg
     4501 ttcgtcccca ggtcccagcg atacagcttc ccgtcttcgc tgttggccag gatcgaatcc
     4561 gtcgatggat cgaccaccgc ggaattgatg caccactcgc gcaccgcgcc gggcttgctg
     4621 ggaaattcac cgtccggcgt tacgccggcg atgctcagca cttccttcat cactgtcgcg
     4681 cctgtcaccg gatcggtctc ggtcgcgttg ggatcgagga tcgccagctt gttcacgccg
     4741 tcgccgccca ggccggcgta gttgttgtac ttggtcatca gcaggtacga cgacggcccg
     4801 gcgtatccgg gcaccatcga cgcggggacg atcgacgccg tgtcgtccca gccgaacgcg
     4861 ccggttgttt tctgtgccga cagatcgccg gtgtagtgca gcatccaccc gcgatcgtga
     4921 ttgctgggga acgggttctc cagcacgccc atgtagacat gcccgtccgg gccgacggtc
     4981 ggcgacgcgc tgccgtcgtc tgtcacctcg gcgtcggccc cggaggccgg gtctttcaat
     5041 cgcacgtgcg cctgcggcgc cagcgtcgag ctgttgagcg agaccagatc gcccacgccg
     5101 ccgcccgtca gggtgatata gagcttcgat ccatcgcggc tcagcgccgg cgcgcaattc
     5161 atgagatcgt tgttgatcac cgcgtcaccc cccgcggccg ccgcggcgga cgtgaaggtc
     5221 tcgctgccat cggcgccgat ccgcgcgatg ccgctgctca agcccgcgcc cagcggcgtt
     5281 gaaccggtca cgcgaaagcc gaaataaatg ttgcccgaag catccgacgt gatcggcgtg
     5341 ttgatgaaga cgttggagtt gtacgccgac ggattcgccg cgtagttggc gtcaccgaag
     5401 aacgccagct ggtgggccgc gctgggcgct gccccgtcga gcgtgtcgcg ccagtaaatc
     5461 gtcccgcccg cgccggcgta gtacatgcga ttgttcggcg tcagcgccgg cgaataacta
     5521 ggcacccagt tgtgcggcgg cagcacgtaa tcgctcgtca cgctccactt gctcgacccg
     5581 gtcgcgccgt tgatcgcatt gatctcaaac ccgccgctcg cgcccgtttt caccggcacc
     5641 agcagcgtgt tcgccgcggt gatcgtcggc gaaccgtagt ggatcagcag atcgttcccg
     5701 ctgaattgcg gcgacagatc gaccggcgtc tgccagcgga tagaagacag aggatccgat
     5761 gccgccggcg cgttggcggc gtgaaatgga tcattcgcgt acccgctcca agccggggca
     5821 gcagcgcgcg tgctaccaac aaatgtcagg ggaaacatcg cgccggcgag cgcggcggtt
     5881 ctgcaacggc gtcgattcat cggccctcca ccgcgaagtt tacccgaaac cccggtcgtt
     5941 tcctcaaaca aagcccacag ttctgccgtg ggaccgtccg ggaaaccagt caccgctgat
     6001 tctcgatacg gcgctgaata acctggaaga gaatgcgcaa acgctgggac ggccatgtcc
     6061 gtgcgtctgc gcattctcct ccagcctatt ggacgccgca gcgattatca gcggcgacca
     6121 atcctgtagc cgttgttttc ccgcggcatc gcatttctaa tcaatcattc aaatgcccgg
     6181 cgagttcgat tttattgagt ggattcgcag ccaacagagg ccgtcgcatc tggtcaacac
     6241 cggcgtcggt gatgacctgg ccgtgctgaa atggccgaag gacgatctgc tgattgccgg
     6301 cgtcgatcag gtgctggacg gcgttcattt tgattcgaag atgcatccgc cgcgcgcgat
     6361 cggccggaag gtgatgaacc gcaacctcag cgactgcgcc gcgatggcat ccctgcccgc
     6421 ggcggccgtc gcgaccgtcg cgctgccgcg aggtgtcggc catgattacg cgaagcagct
     6481 gttcctcggg atgaaggaag cgggcgaggt gttcgactgc gcgatcgtcg gcggcgacac
     6541 cggatcgtgg gacggcaagc tcgtgatgac cgtcacgatc ctcggccgca gcgccggcgt
     6601 tcagccggtg ctgcgcagcg gcgccaaagc cggcgacggc atctacgtct ccggtcccct
     6661 tggcggaagc atcctgggcc ggcacatgac gttctcgccg cgcgttgatc tcgggcgcga
     6721 gctggccaaa cgcggtgacg ccgcggcgat gatcgattta agcgacggac tatctcgcga
     6781 tctccgccac atctgccgcc tgagcggcaa cctcggcgct gtcgtctacg ccgatcgcgt
     6841 gccgattcac gatgatgcgc aggcggcatt ccgcctcgac cacgtgtcgc cgctcgatca
     6901 tgcgctcaac gacggcgaag actacgagtt gctgtacacc tgccggaacg tggcgaccat
     6961 gggcatctac atcggcgaca tcagcagcgc gcccggcatc tggctgcaac acgccgacgg
     7021 caagcgcgaa ccgctggaac ccaaggcgtg ggagcattta ctctgaacag cgcggatgtc
     7081 cgatcggcgc cgactgaaga aaagcccacg gatcgctatc cgtgggcctt aatgctgccg
     7141 tacgcgatca caatttttct cggcgcgttc ctgctcttcc aagtccaacc gctgatcggc
     7201 aaatacatcc ttccctggtt cggcggcgcc accgcggtgt ggacgacgtg catgctgttc
     7261 ttccagctgc tgctgctcgg cggctacgcg tatgcacacc tctcgacgca gctgctgcgg
     7321 ccgcgcgctc agtcgatgct gcacgtcgcg ctgctcgtcg cggcgttgtg cgtgctgccg
     7381 atcgttccgt cgccgcgatg gaaaccgacg ccggcggacg
//
LOCUS       DATIMH010000297         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5266795, whole genome shotgun sequence.
ACCESSION   DATIMH010000297 DATIMH010000000
VERSION     DATIMH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5266795"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="VLI90_05065"
     CDS             <1..1768
                     /locus_tag="VLI90_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSV13607.1"
                     /translation="QANPVIMDKLRELGLLLGQETITHSYPHDWRSKTPIIFRATEQW
                     FVAMDKPFTLFPVAATPASPSGERATQASPLQSEEPRSLRQRAMDAVNKGIEFVPGWG
                     QARMRGMLESRPDWCISRQRAWGLPIPVFYNEKGEPLLTPESVRAVAKRFEEKGSDVW
                     FTDPPTELLGCDQTVCPTHFGDMATLRKEQDIFDVWFESGSSWHAVLQERSYLKYPAD
                     LYLEGSDQHRGWFQLSLLPSLGATGQPPFKQVLTHGFVVKPDGTKVSKSDKEYVKAVD
                     EINRHGADLLRLWCCSVDYQNDIPASPKAIQEFGDKYRKIRNTLRYLLSNLYDFNGRT
                     HCVEVSANSLDGWAIDQLDKLIVDVRAGYDAYQLHRVFRLLHDFCSVQVSAVYGNAMK
                     DRLYCEAADAPLRRRCQTVMYRMVIALTKLLAPMIVFTADEAWQHIAHRPADEADLPS
                     VHLALLPEPSRQQVSDEVREQWSLLMQLRDAALLQLDALKKEAGLNKALESEVVYHLG
                     DNRLRQSLQAYGPDLEDLVGAGHHAFSEDQSPHPAVKVFDTRSKCGACARCWKRRPDV
                     GQDAEFGDLCLRCAAAVRTSSR"
     gene            1863..2492
                     /gene="purN"
                     /locus_tag="VLI90_05070"
     CDS             1863..2492
                     /gene="purN"
                     /locus_tag="VLI90_05070"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HSV13608.1"
                     /translation="MSAASPIKLAVLVSGSGTTLQNLIDEIAAGRLNASIQIVIGSRP
                     DLVGIERAAAAKIPNFVVDRKGFDSCTAFSKQVFGLVDDAGVDLVCLAGWLCLLDLPE
                     KYAGKAINIHPALLPSFGGKGMFGQRVHQAVIDHGCKVSGCTVHFVDAAYDSGPIIIQ
                     RTCPVLDDDAAETLAHRVFEEERIAYPQAVRLFQAGKLKIEGRRVRTLS"
     gene            complement(2536..3357)
                     /gene="trpA"
                     /locus_tag="VLI90_05075"
     CDS             complement(2536..3357)
                     /gene="trpA"
                     /locus_tag="VLI90_05075"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSV13609.1"
                     /translation="MATTAVSRQSIRSTFQALRQRKQIALMPFVPAGYPDLAATAAVL
                     PAIEAAGASLIEIGIPFSDPIADGPTIQAAFTEALAKKLKVADIFRTIAEVRPRMSIP
                     LVAMVSYSIVFRYDLNRFLKDMKAAGFDGLILPDLPPPEARRVCDAVQAAGLDTILLV
                     APSTTAERRKEIARLSSGFIYYLSVAGITGERDRLPADLERNVAELKSLTDRPVCVGF
                     GIHKAEQVAELAKFADGAIVGSAIVTRMKQHAPQGADAIAKAVEGYCRELLRLVR"
BASE COUNT          679 a         1056 c         1146 g          651 t
ORIGIN      
        1 gcaggccaac cccgtcatca tggataagct gcgcgagctc ggactactgc tgggccagga
       61 gacgatcacg cacagctacc cgcacgactg gcgcagcaaa actccgatca tctttcgcgc
      121 aacggagcaa tggttcgtcg cgatggataa gccgttcacc ttgtttcctg tagctgcgac
      181 gcctgcgtcg ccttcgggag aaagggcgac gcaggcgtcg cccctacaga gcgaggagcc
      241 gcgctcgctt cggcagcgcg cgatggacgc ggtcaacaag ggcatcgaat tcgtccccgg
      301 ctggggccag gcgcgaatgc gcggcatgct cgaaagccgc cccgattggt gcatctctcg
      361 gcagcgggcg tggggcttgc ccattccagt cttctacaac gaaaagggcg aaccgctgct
      421 gacgccggaa tcggtgcggg cggtcgcgaa gcgattcgaa gagaagggat cggacgtgtg
      481 gttcaccgat ccaccgacgg aattgctcgg ctgcgatcaa acggtctgcc cgacacactt
      541 tggcgacatg gcgacgctcc gcaaggagca ggacatcttc gacgtctggt tcgaatccgg
      601 cagcagctgg cacgcggtgc tgcaagagcg gtcatatcta aaatacccgg cagacctgta
      661 cctcgaaggc tccgatcaac accgcgggtg gtttcagttg tctttactac catcgcttgg
      721 cgcgacgggt cagccgccgt tcaagcaggt gctgacgcat gggttcgtgg tgaagccgga
      781 cggcacgaag gtcagcaaga gcgacaagga atatgtgaag gcggtcgacg agatcaaccg
      841 ccacggcgcc gatctgctgc ggctgtggtg ctgcagcgtc gattaccaga acgacatccc
      901 ggccagcccg aaggcgatcc aggagttcgg cgacaagtat cgcaagatcc gcaacacgct
      961 gcggtatctg ctgagcaacc tgtacgactt caacggccgc acccattgcg tggaggtgtc
     1021 ggccaacagc ctggatggct gggcgatcga tcagctggac aagctgatcg tggacgtccg
     1081 cgccggctac gacgcgtatc aactgcatcg cgtgttccgg ttgctgcacg atttctgctc
     1141 ggtgcaggtc agcgcggtct atggcaatgc gatgaaggac cgcctgtatt gcgaagccgc
     1201 cgatgcgccg ctgcggcggc gttgccagac ggtgatgtat cgcatggtca tcgcgctgac
     1261 gaagctgttg gcgccgatga tcgtcttcac cgccgacgaa gcctggcagc acatcgcgca
     1321 tcgcccggcg gacgaggcgg atctgcccag cgtacacctc gcgctgctgc cggaaccatc
     1381 gaggcagcaa gtgagcgacg aggtgcgcga gcaatggtcg ctgctgatgc agctgcgcga
     1441 cgcggcgttg ctgcagctgg atgcgctgaa gaaggaagcg gggctgaaca aggcgctgga
     1501 gtcggaagtg gtgtatcacc tcggcgacaa caggctgcgg caaagccttc aggcgtatgg
     1561 gccggacttg gaagacctgg tcggagccgg ccatcacgcg ttctcggaag atcaatcgcc
     1621 ccatccggcg gtaaaagtgt tcgacacgcg gagcaagtgt ggcgcgtgcg cgcgatgctg
     1681 gaagcgccgg ccggacgtgg ggcaggacgc ggagtttggt gatctgtgtt tacgctgcgc
     1741 cgcggctgtg agaacgagtt cgcgctgaga ctgtaaaaaa gcccacggct acgccgtggg
     1801 acggattcta aaagactatc ccacggcaat agccgtgggc tttcgcatcg ggcgcaccgt
     1861 aaatgtctgc agcatccccc atcaaactcg cggtgctcgt cagcgggtcc ggcaccacgc
     1921 tgcagaacct catcgacgaa atcgccgcgg ggcgattgaa cgcgtcaatt cagatcgtga
     1981 tcggttcgcg gccggacctg gtaggcatcg agcgcgctgc ggcggcgaag attccgaact
     2041 tcgtcgtcga ccgaaaaggt tttgactcgt gcacggcgtt cagcaagcaa gtgttcggat
     2101 tggtcgatga cgccggcgtc gatctggttt gtcttgccgg ctggctttgt ttgctcgatc
     2161 taccggagaa gtacgccggt aaggcgatta acatccaccc cgcgctactg cccagcttcg
     2221 gcgggaaggg catgttcggc cagcgcgtgc atcaggcggt gatcgatcac ggctgcaagg
     2281 taagcgggtg cacggtccat ttcgtcgatg ccgcgtatga cagcgggccg atcatcattc
     2341 agcgcacctg tcccgtgctg gacgacgacg cggcggagac gctggcgcat cgggtgttcg
     2401 aggaagagcg gatcgcgtat ccgcaggcgg tgcgattgtt tcaggcgggg aagttgaaga
     2461 tcgaggggcg ccgcgtgcgg acgttatctt gaaaaattaa agcccacgga tagctatccg
     2521 tgagctttcg tgtggtcatc gcaccaatcg caataattcg cggcagtatc cttctaccgc
     2581 tttcgcgatc gcatccgcgc cttgcggcgc gtgctgcttc attcgcgtga cgattgctga
     2641 tccaacgatc gcgccgtccg caaatttcgc cagctcggcg acctgctccg ccttgtgaat
     2701 cccgaacccc acgcacaccg gccgatcggt caacgacttc agctccgcga catttcgctc
     2761 tagatcagcc ggaagccgat cgcgctcgcc cgtgatgccg gcgacggaga ggtagtagat
     2821 gaagccgctg ctcagtcgcg cgatttcctt tcggcgttcg gctgtcgtgc tcggcgcgac
     2881 cagcaagatg gtatccagcc ccgcggcttg cacggcgtcg cacacccgcc gcgcctccgg
     2941 cggcggcagg tccggcagga tcagcccgtc aaatcccgcg gccttcatgt ctttcaggaa
     3001 tcgattcagg tcgtagcgga acacgatgct gtagctgacc atcgccacca gcgggatcga
     3061 catgcgcggg cggacttccg cgatggtgcg aaaaatgtcg gcgactttca gtttcttcgc
     3121 taacgcttcg gtgaacgccg cttgaatggt tggcccatcg gcgatcggat cggaaaacgg
     3181 aatgccgatt tcgatcaagc tcgcgcccgc ggcttcgatg gccggcagca ccgccgccgt
     3241 cgcggcaagg tcgggatatc cagcggggac aaacggcatc agcgcgatct gcttgcgctg
     3301 ccgcagggct tggaatgtcg agcgaatgga ttgacgagac acggcggtgg tggccatggc
     3361 cgcatcctgc gggcgatcag cggcggcgtc aacggcattg ctctacgagt agctcgcaac
     3421 cctttccgaa gagttgccca ggcgtcgact agccgtcgcg ggcggttcgc tgaaactgcc
     3481 tagcggagtt gctcaagctc agatcgcagc gccgcctcca atagcaccat ct
//
LOCUS       DATIMH010000298         1900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1511545, whole genome shotgun sequence.
ACCESSION   DATIMH010000298 DATIMH010000000
VERSION     DATIMH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511545"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1446)
                     /locus_tag="VLI90_05080"
     CDS             complement(106..1446)
                     /locus_tag="VLI90_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSV13610.1"
                     /translation="MKRIAITIVAAALLAGCTVHPKGEREERDIAIAAGKTYEKPVEE
                     RQLPPLSDRPTADDLVRFALLNSAEVERAYWEWRAALEQVPIDGTQATNLSLSLSSSV
                     VNGNTSWGQTTITAGNDPMADIVLPNKLDAAARRSLEMARAAGKRFHKMQLELRAKVL
                     DAYYDYALNAELIRLSRQNIAWLQITRTLADTRVRTGGGSQAELLKAENELDMALNDL
                     ANLQAQLPANRAMINALLNRPALADLPVPDAVPSTGPVNGDDAAILGAIDSDNPELKA
                     LAHEIRSRKVGIETASLQYQPDFSISAATDLRGITQTLSSMITVPILKYEAIHATIAQ
                     AEANLKAAEAARRQSKSDLAAQAISDLVTLRDVDRQLSLFEQTILPRAKQIVPVTRQS
                     YETGRGSLLELFDAQRSLLDIERLVANLRTTRNKRLASIEAMGAPLYPVPANTH"
     gene            complement(1443..>1900)
                     /locus_tag="VLI90_05085"
     CDS             complement(1443..>1900)
                     /locus_tag="VLI90_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV13611.1"
                     /translation="VVLLAVPFSLVGAVWLLWFLGYHLSLAVWVGMIALAGLDAETGL
                     VMLLYLDNSYERFNSERRMNNRDDLWHAVHDGAVKRIRPKTMTVAAAFIGLVPLLWAT
                     GTGADVMRRLAAPMLGGLFTSFLMELLIYPIIFYLAKAMTLQRETKEVA"
BASE COUNT          312 a          617 c          607 g          364 t
ORIGIN      
        1 ggagcgtact cgcgcagtaa gtggcgcgcg cagcgcttac tgcgcgagta cgctccgtgc
       61 gcggctcctt tgcggtgcaa tagcttccat tatgcatcct gcacttcaat gcgtattggc
      121 cggcacggga tagagcgggg cgcccatcgc ctcgatgctc gcgaggcgct tgttgcgcgt
      181 cgtgcggagg ttggcgacga gacgttcgat gtccagcagt gatcgctgcg cgtcgaacaa
      241 ctccaacagc gacccgcggc cggtttcgta ggactggcgt gtcaccggca cgatctgctt
      301 cgcccgcggg aggattgtct gttcgaacag cgatagctgt cggtccacgt cgcgcagtgt
      361 caccagatcg gagattgcct gggcggccag atcgcttttt gactgccggc gggcggcctc
      421 ggcggctttg aggttcgcct ccgcctgcgc gatggtggca tggatcgctt cgtacttcag
      481 aatcggaacc gtgatcatgc tgctcagcgt ttgcgtgatg ccgcgcaggt cggtggcggc
      541 ggagatggaa aaatcgggct gatactgcag gctggccgtc tcaatgccga ccttgcggct
      601 gcggatttca tgggccagcg ctttgagctc cggattgtcg ctgtcgatcg cgccgagaat
      661 cgcggcgtcg tcgccattca ccggcccggt gctcggcacc gcatccggca ccggcaggtc
      721 agcgagcgcc gggcgattca acagcgcgtt gatcatcgcg cggttggccg ggagttgcgc
      781 ctgcaggttc gccaagtcgt tcagcgccat gtcgagttcg ttctccgcct tcagcaactc
      841 cgcctgcgag ccgccgcccg tgcggacgcg cgtgtcggcc agcgtgcgcg tgatctgcag
      901 ccaggcgatg ttctgccggc tcaggcgaat caactccgcg ttcagcgcgt agtcgtaata
      961 cgcatccagc accttggccc gcaattccag ctgcatctta tggaatcgct ttccagcggc
     1021 gcgggccatc tccagcgaac gccgggcggc ggcgtccagc ttgttgggca ggacgatgtc
     1081 cgccatcggg tcgttcccgg ccgtgatcgt cgtctgtccc catgaggtgt tgccgttgac
     1141 gacgctgctg gatagcgaaa gcgacaggtt ggtcgcctgc gtgccgtcga tgggcacctg
     1201 ctccaacgct gctcgccatt cccaataggc acgctcgacc tccgcgctgt tcagcagcgc
     1261 gaagcggacc agatcgtcgg ccgtcgggcg atcagatagc ggcgggagct gccgttcctc
     1321 gaccggcttt tcatacgtct tccccgcggc gatggcgata tcgcgctcct cgcgctcacc
     1381 cttgggatgc accgtgcaac cggcgagcag cgcggcagca acgattgtga tcgcaatgcg
     1441 cttcatgcga cctccttcgt ttcccgctgc agcgtcatcg ccttggccag ataaaagatg
     1501 atcgggtaaa tcagcaactc catcaggaag ctcgtgaaca gcccgcccag catcggggca
     1561 gccagtcttc gcatcacatc cgcgccggtg ccggtggccc acagtagcgg caccagcccg
     1621 atgaacgcgg cggccaccgt catcgtcttg ggacgaatcc gcttcaccgc gccgtcgtgc
     1681 acggcgtgcc ataggtcgtc gcgattgttc atccgccgct cggagttgaa gcgctcgtag
     1741 ctattgtcga gatacagcag catgaccagg ccggtttcgg catcgagtcc cgccagcgcg
     1801 atcatgccga cccacaccgc cagcgacagg tgatagccga ggaaccagag caaccagacc
     1861 gcgccgacaa ggctgaacgg cacggccagc agcacgacgc
//
LOCUS       DATIMH010000299         6589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1801184, whole genome shotgun sequence.
ACCESSION   DATIMH010000299 DATIMH010000000
VERSION     DATIMH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801184"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VLI90_05090"
     CDS             <1..822
                     /locus_tag="VLI90_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13612.1"
                     /translation="VLDAMGFDGFLKEVERHLPTGITLRRFPLDKCQARPTVQKHGHI
                     GFHAQKQSGLYYVGVVLPVGRMTVQQMRGLADIAERFGSGTIRLTVWQNLLISDIPAA
                     CIDEAKASLRAIGLDYSTSSLRAGLVACTGNAGCKYAAANTKAHALVLANYLEHRVQL
                     DQPINIHLTGCHHSCAQHYIGDIGLIATTIAVGEEMVEGYHLFVGGGYGAEQGIAREL
                     YRDVTASELPSLLDRVLQGYLEHRNAPGESFRQFVLRHTSDALKEVFERTAAAAA"
     gene            842..2389
                     /locus_tag="VLI90_05095"
     CDS             842..2389
                     /locus_tag="VLI90_05095"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004859.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HSV13613.1"
                     /translation="MSTTLLLPESAPFTPQQRAWLSGFFTGLVNVQPEPVAAAPAVEE
                     EFPWHDPALSLSKRLALAAGKPRERQLMAAMAQLDCGACGYLCQTYAEAIASGSEKDL
                     ARCTPGGRETNKSLKELLKVLPSDAGQAKSAPAAPVKKQEQAAHGRDNPFPARLVQCK
                     PLNGAGSSKDTRHVVLDLRHSNLSYKVGDALGVYPENCHDTVQQLLEALHTSGAEDVE
                     NPAGEHVSLRHALLHDITITRPSDALLELLASSASDAEQKVELTKLAADGLAEDDTRQ
                     VIDLLRQFPSARPMAEAFVAALLPLQPRLYSISSSLLAHPNQVHLTVGVVRYMNGAGR
                     TCKGVASNYLTERVRPGQHVRVFVHASPKFCLPSDRNAPVIMVGPGTGIAPFRAFLHE
                     RRVDRHPGKNWLFFGDQRSECDFLYRDELQTMHEEGVLTRLDTAFSRDQAEKVYVQTR
                     MMQSAGELWSWLQAGAYFYVCGDAKRMAVDVDRALKQIIAEQGSVDADVFVAAMTRNG
                     RYQRDVY"
     gene            2460..4628
                     /locus_tag="VLI90_05100"
     CDS             2460..4628
                     /locus_tag="VLI90_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HSV13614.1"
                     /translation="MPHTRDAKTLCPYCGVGCGIIATTDGSRITKIRGDNHHPANFGK
                     LCPKGATVAQTVDVRTRLRHAMSRSSRAQEPSRVTAGEAVQHVADRLNHILQSHGPEG
                     IAFYLSGQLTTESQYLFNKFAKGYLRTNHVDSNSRLCMSSAASAMTLSLGSDGPPTCY
                     ADIDLADTFLFIGSNAAECHPIVFGRVERRLEKRRTHCVVVDPRRTKTAESANLHLAV
                     RPGTDLALLNGLLYLLDMWGKIDPAFIAAHTEGWAELRAMLPQYPPHRVSQICGIGVP
                     ELLQTAKTIADAKRLITFWTMGVNQTLQGTFTSNAIINLHLATGQIGKPGCGPFSLTG
                     QPNAMGGRDVGYMSHLLPGQRQIANADHRLQIERFWGLPQGTIYPHAGYDAVRMFDAL
                     ESRDIRAIWIVGTNPAASMPNLPKIRRALERADLVIVQDAYYPTETTRYADVLLPAAV
                     NLEQSGTFCNSERRVTLMEQVVKPPGEAQPDWWWPRHVAAAMSFTRGMWFNTAEDIFD
                     EFARSTAGRPNDQSALSHATLRAKGPQIWPCPALGAPQERRYTDGVFPTSSGRARFFA
                     REHLTAEEKTTAKFPLLLTTGRVANQWHTRTKTALVPALGKGEAAPSVHMHPADAESL
                     FLQDRQAVTVHSSRGESATILRIDAAMSPGLVWMPIHWNELWGKRASCNEITTDASDL
                     QSKQPALKCCAVSVTARADDAAEPETATAVAPLAIVHRHV"
     gene            complement(4953..5024)
                     /locus_tag="VLI90_05105"
     tRNA            complement(4953..5024)
                     /locus_tag="VLI90_05105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4990..4992),aa:Gly,seq:tcc)
     gene            5400..>6589
                     /locus_tag="VLI90_05110"
     CDS             5400..>6589
                     /locus_tag="VLI90_05110"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSV13615.1"
                     /translation="MLLSESASAQLQLVSTTGTQANPVFHYDITVTDTGTTNIGTFWF
                     SWVPEENFLPTSPAAASSPAGWSATITGFGNSIDGAGIEWVATSNAITPGHSLSGFDF
                     TTSDSPSALAANSPAHPGLPVLTAFVYSGGPFSDLGSQVSVTTVAAQVASMTTLATSA
                     PTITAGDSLTLTATVAPAAGSGATPTGTVTFFEGSNNLGTSQVQSDGAAALVTAALPS
                     GTLSLTAQYSGDSAYTGSTSAAITQIVTPAGTAATTVALSSSAPAPAPGTPVTVNATV
                     SAVTSNGSTPSGNVSFFDNGAPAGEAALVDGVAQVPLTLAVGVHAITATYAGDATFAG
                     STSTAGVTETVTAAPTLSAAIAKDTLPSVFVPGDRGLVALQLTNAASILERGTVAIQA
                     FLTP"
BASE COUNT         1252 a         2100 c         2104 g         1133 t
ORIGIN      
        1 gtgctggatg caatgggctt tgacggcttc ttgaaagaag ttgaacggca tttgcccacc
       61 ggtatcactc tgcggcggtt cccgttggac aagtgtcagg cgcgccccac cgttcagaag
      121 catggccaca ttggatttca cgcccagaaa caatcgggtc tttattatgt cggcgttgtc
      181 ctgcccgttg ggcgaatgac ggtgcagcag atgcgcggcc ttgccgatat cgccgagcga
      241 ttcggcagcg gcacgattcg gcttaccgtc tggcagaacc tgctgatctc cgacattccc
      301 gccgcatgca ttgatgaggc gaaggcgtcc ctccgcgcga tcggactgga ttattcgacg
      361 tcgtcccttc gcgccggact cgtcgcctgc accggcaacg ccggttgtaa atacgcggcg
      421 gccaacacca aggcccacgc gcttgtcttg gcgaactacc tcgagcatcg cgtccaactt
      481 gatcagccga tcaacatcca cttgaccggc tgtcaccatt cgtgcgcaca gcattacatc
      541 ggcgatatcg gcttgatcgc cacgacgatc gccgtcggcg aggagatggt cgaaggctat
      601 cacctgttcg tcggcggtgg gtacggcgct gagcagggaa tcgcgcgcga gttgtatcgc
      661 gacgtgacgg cgagcgagtt gccgtcgctg ctcgatcggg tgttgcaggg atacctcgaa
      721 catcgaaatg cccccggtga gtcattccgg caattcgtgc ttcggcacac gagcgatgcg
      781 ttgaaggaag tgttcgaacg tacggcggct gcggccgcat aagcccataa ggagcagata
      841 aatgtccacc acgttgttac tgcccgaatc cgcgccgttc acaccgcagc agcgcgcgtg
      901 gttgagcggc ttcttcaccg gcttggtgaa tgtgcaacct gaacccgttg ccgccgcacc
      961 ggcggtggag gaagaatttc cctggcacga cccggcgtta tcgctttcca aacgcctggc
     1021 gctggcggcg gggaaaccgc gggaacggca gctgatggcg gcgatggcgc agctggattg
     1081 cggcgcgtgc ggctacctct gccagacgta tgccgaggcg attgcatcgg gcagcgagaa
     1141 ggacctggcg cgctgcacac ccggtggccg cgagacgaac aagtcgctca aagaactgct
     1201 gaaggtgctg ccgtcggatg cggggcaggc gaagtctgcg ccggccgcgc cggtgaagaa
     1261 acaggagcag gcagctcatg gccgcgataa tccgttccct gcgcggctcg tgcagtgcaa
     1321 gccgctgaat ggcgcgggat catcgaaaga cacgcggcac gtcgtgctcg atcttcgcca
     1381 cagcaacctg tcgtacaaag tcggcgacgc gctgggcgtt tatccggaga actgtcacga
     1441 cacggtgcaa cagctgctcg aagcgctgca caccagcggc gccgaagacg tggagaaccc
     1501 cgcgggtgag cacgtcagcc tgcgccatgc gttgctgcac gatatcacga tcacacggcc
     1561 gagcgatgct ttgctggaat tgctcgccag cagcgcatcc gatgccgagc agaaagtgga
     1621 actgacgaag ctcgccgccg acggcctggc cgaggatgac acgcggcagg tcatcgatct
     1681 gctgcggcag ttcccgtccg cccggccgat ggcggaggcg ttcgtggccg cgctgttgcc
     1741 gctgcagccg cggttgtatt cgatatcgtc atcgctgctg gcgcatccga accaggtgca
     1801 tctgacggtg ggcgtggtgc gttacatgaa tggcgccggg cggacgtgca aaggcgtcgc
     1861 gtcgaactat ctgaccgagc gcgtgcggcc ggggcagcac gtgcgcgtgt tcgtgcacgc
     1921 atcgccgaaa ttctgtctgc cgagcgaccg caatgcgccg gtcatcatgg ttggcccggg
     1981 caccggcatc gcgccgtttc gcgcgtttct ccacgaacgc cgggttgacc ggcatccggg
     2041 caagaactgg ttgttctttg gcgaccagcg gagcgaatgc gatttccttt atcgcgatga
     2101 actccaaacg atgcacgaag agggcgtgct cacgcggctc gacacggcgt tctcgcgcga
     2161 tcaagccgag aaggtgtatg tgcagactcg aatgatgcag agcgccggcg aattgtggag
     2221 ttggctgcag gccggggcgt acttctacgt ctgcggcgac gccaagcgaa tggcggtgga
     2281 tgtcgaccgg gcgctcaagc agatcatcgc cgagcaaggt agcgttgatg ccgatgtgtt
     2341 tgtggcggcg atgacgcgga acggccgata ccagcgcgat gtctactagc cgcttcgcgg
     2401 cttagcatcg ttccccccga aaacaaatgc taagccgcga agcggcagga agccgacaat
     2461 tgccccacac ccgcgacgca aaaactctct gcccctactg tggtgtcggc tgcggcatca
     2521 tcgcgacgac cgacggttcg cgcattacga agatccgtgg cgacaaccat caccccgcga
     2581 acttcggcaa gctctgcccc aaaggcgcca ccgtcgcgca gacagtcgat gtccgcacgc
     2641 gcctgcgtca cgcgatgagt cgctcgtcgc gcgcgcaaga gccgtcgcgc gtcaccgccg
     2701 gggaggcggt tcagcacgtc gccgatcggc tgaaccacat cctgcagagc catgggcctg
     2761 aaggcatcgc cttttatctc tccggccaac tcaccaccga atcgcaatac ctgttcaaca
     2821 agttcgccaa aggatacctg cggacgaacc acgtcgacag caacagccgg ctgtgcatga
     2881 gcagcgcggc tagcgcgatg acgctgtcgc tcggcagcga cggcccgccg acctgctacg
     2941 cggacatcga cttggccgac acgttcctgt tcatcggctc caacgccgcc gagtgtcacc
     3001 cgatcgtgtt cggccgggtt gaacgtcggc tggaaaagcg ccgcacgcac tgcgtcgtcg
     3061 tcgacccgcg aagaacgaag acggctgaat cggcaaatct gcatttggcc gtgcgacccg
     3121 ggacggattt agcgctgctc aacgggctgc tgtatctgct cgacatgtgg ggcaagatcg
     3181 acccggcgtt catcgcggcg cacaccgagg gttgggcgga gctgcgggcg atgctgccgc
     3241 aatacccgcc gcatcgtgtg tcgcagatct gcggcatcgg cgtgcctgaa ctgctgcaaa
     3301 cggcgaagac gatcgccgac gccaagcgcc tcatcacctt ctggacgatg ggcgtgaatc
     3361 aaacgctgca aggcacattc acgtcgaacg cgatcattaa cctgcatctg gcgacgggcc
     3421 agatcggcaa gccgggttgt gggccgttca gtttgaccgg tcaacccaac gcgatgggcg
     3481 ggcgtgacgt cggctacatg agccacctgc tgccgggcca gcggcagatc gccaacgctg
     3541 atcatcgctt gcaaattgaa cgcttctggg gcttgcccca agggacgatt tatccgcacg
     3601 ccggatacga cgcggtgcgc atgttcgatg cgctggagag ccgcgacatc cgcgcgatct
     3661 ggatcgtcgg caccaacccc gcggcgagta tgcccaacct gccgaagatc cgccgcgcgc
     3721 tggagcgcgc ggacctggtg atcgtgcagg atgcctatta cccgacggag acaacgcgat
     3781 atgccgacgt gctgctgccg gccgcggtga acctggaaca atccggcacg ttctgcaaca
     3841 gcgagcggcg cgtgacgctg atggagcagg tggtgaaacc accgggcgag gcgcagcccg
     3901 actggtggtg gccgcggcac gtggcggcgg cgatgagctt cacgcgcggc atgtggttca
     3961 ataccgccga agatattttc gatgaattcg cccgcagcac cgccggccgg ccgaacgatc
     4021 agagcgcgct gtcgcatgca acactgcgcg cgaagggccc gcagatctgg ccgtgcccgg
     4081 cgctgggcgc cccgcaagag cggcgctata ccgacggcgt cttcccaact tccagtggac
     4141 gagcgcgctt ctttgcccgc gagcatttga cggcggaaga gaaaaccacc gcaaagttcc
     4201 cgctgctgct gacgaccggc cgcgtcgcca accaatggca tacgcgcacc aaaacggcac
     4261 tcgtgcctgc gctgggcaaa ggggaagcgg cgccgagcgt tcatatgcat ccagcggatg
     4321 cagaatcgct gttcctgcag gaccgtcaag ccgtaaccgt ccacagttct cgcggcgaat
     4381 ctgccacaat ccttcgcatc gatgccgcaa tgagcccggg gctcgtgtgg atgccaattc
     4441 actggaacga gctgtggggc aagcgcgcca gttgcaatga gatcacaacc gacgcgtcgg
     4501 atctgcaaag caagcagccc gcgctgaaat gctgcgccgt ttcggtcacg gctcgagcag
     4561 atgatgcagc cgagccagaa accgctacgg ctgtcgcgcc gctcgccatc gtgcatcgtc
     4621 acgtgtaaag atccaactgc tgcccttcaa gccgcttcct ctggcaagac aagctaaggt
     4681 gcgcgcgctg acggagcagg agacgacgaa cctgttgaac gccgtaccag ctcgacatca
     4741 gttggcttat cgactgatcc tcagcaccag gctgcgccgg gattcaggaa actgcgccgg
     4801 ggcgcagcga aagcatcagt agccgattgt ccgatttcgg gggttatgcg gcagtgatcg
     4861 gcgacaacga aggcgcgctc cacattacgc aaaccgcttg cgctggcggg cattggcaac
     4921 aaaagacccc atcggctgcg atggggaaaa tgagcgggcg aagggactcg aaccctcaac
     4981 agctagcttg gaaggctagc gctctgccat tgagctacgc ccgcgaatcg cgcgcagtac
     5041 gttagcacac caaatgccag ggttcaaata tgcgcggatg ttgcgatgat ccggcggatt
     5101 ccggcatatc ccgaacccga catgcaaggg gcgtcatcgc gctctcgcct cgttcgctca
     5161 gaagagcgct catttcctct tcgtgtcccg ggcgacgggc cttggcggct tctgaacatg
     5221 caaattctgc gcaccgcaag ttcaggctgt tatggtgacg cgcgcgccgc ggagccccgg
     5281 caacctgagg taacgagatg tttggatcga tcgttcgaca agcgatgtct tcacgcgctg
     5341 caactgcacg gaacgccgtc cggcgacacg cctgctgcac ggaatttctt gaatcgcgga
     5401 tgctcctgag cgagtccgcc tccgctcagc ttcaattggt ttcgacgacc ggcacgcagg
     5461 caaatccggt ttttcattac gacatcacgg tcaccgatac cggcacgacc aatatcggca
     5521 cgttctggtt ttcgtgggtg ccggaggaaa actttctacc gacatcgccg gcggccgctt
     5581 cgagtcccgc ggggtggagc gcgacgatta ccggattcgg caactcgatc gacggcgcgg
     5641 ggatcgaatg ggttgcgaca tcaaacgcca tcacgccggg gcattcgtta agcgggttcg
     5701 acttcacgac gagcgacagt ccatcagcgc tggcggcgaa ttcgccggcg catccggggc
     5761 tgccggtgct caccgcgttc gtttacagcg gcgggccgtt ctcggacttg ggctcgcaag
     5821 tcagcgtcac gacggtcgcg gcgcaggtgg cgtcgatgac gacgctcgcg acttccgcgc
     5881 cgacgatcac cgcgggcgat tcgctcacgt tgacggcgac cgtcgcgccg gcggccggct
     5941 cgggcgcgac acctaccggt accgtcacgt tcttcgaagg cagcaacaac cttggcacat
     6001 cgcaggtgca gagcgacggc gcagcggcgc tggtgacggc ggcgttgccc agcggcacgc
     6061 tcagcctgac cgcgcaatac agcggcgaca gcgcgtacac cggcagcacg tcggcggcca
     6121 tcacgcagat cgtcaccccc gccggcacgg cggcgacgac cgtcgcgctt tcgtcatcgg
     6181 ccccagcgcc cgcgccggga acgccggtga cggtcaacgc gaccgtctcg gccgtcacgt
     6241 ccaatggttc gaccccgagc ggcaacgtca gcttcttcga caacggcgcg ccggccggtg
     6301 aggcggcgct ggtggatggc gtggcgcagg tgccgcttac gctcgccgtc ggtgttcatg
     6361 cgattacggc gacatacgcc ggcgatgcga cgttcgccgg cagcacatcg acggcgggcg
     6421 tcaccgagac ggtcaccgcg gcccccacgc tcagcgcggc gatcgccaaa gacacgctgc
     6481 ccagcgtctt cgtcccgggc gaccgcgggc tggtcgcgct gcagctcacc aacgccgcct
     6541 caatcctgga gcgcgggacg gtggccatcc aggccttcct cacgcccga
//
LOCUS       DATIMH010000300         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3532968, whole genome shotgun sequence.
ACCESSION   DATIMH010000300 DATIMH010000000
VERSION     DATIMH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3532968"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VLI90_05115"
     CDS             complement(<1..386)
                     /locus_tag="VLI90_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13616.1"
                     /translation="MSAGAPIICFGQQPCGIFPKRFLYAKIATARRLQQEIGGEVVFF
                     YHDSDHDPRETKTILRHRKNSEAFAINFTFENKLQRKYSPLYLKRVMRDWREKTAIQL
                     PAYVDRCWVEAFKQTDAANVADFCLA"
     gene            complement(383..2077)
                     /locus_tag="VLI90_05120"
     CDS             complement(383..2077)
                     /locus_tag="VLI90_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HSV13617.1"
                     /translation="MNALDYVVLIGSLLGIAAYGMWRTRGRRNLNTYLKGDGRARWIV
                     IGLSVMATQASATTFLSTPGQGFNDGLGFVQNYFGAPFALILIAAVFLPIYRRLKVNT
                     AYEYLGKRFDSKTRLLGAALFLLQRGLGAGITIYAPAIVLSTVFGWRLDLTILCTGLV
                     VIVYTVTGGTDAVTVTQKYQLGVIFAGMITAFAVLVAKLPHDLHFGEALTIAGRFHKL
                     NGVTFSTDLHQRYTFWSGTIGGMFLALSYFGTDQSQVQRYISGASLRESRLGLMFNAV
                     FKIPMQFFILLLGVLLFVFYQFQAPPVYFNRTAWVDQENRGDAPAMRAMEAAFADAHA
                     RKREQIDAWLRARRDGDAAAAASAKRELLAMDRRADEVRARAKSLMTAANRTNANDND
                     YVFITFILDQLPHGVIGLLVAAFFAAAMSSKAAELNALGSTTTVDFYRHVIRRSASDA
                     HFVFATKCFTVFWGLVAIGFALFAHLSENLIQAGNIIGSVFYGPVLGLFLVAFFLRWI
                     GGTAIFVSTLVAQVLVLLFYYHLTISYLWYNIIGCAACMLLAIVLQAMLRRGTTNV"
     gene            complement(2074..2220)
                     /locus_tag="VLI90_05125"
     CDS             complement(2074..2220)
                     /locus_tag="VLI90_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13618.1"
                     /translation="MTADPGVSTESDDTGLPALRTWPAVYLFVLVVFVIWVALLIALE
                     RTYS"
     gene            complement(2217..>3120)
                     /locus_tag="VLI90_05130"
     CDS             complement(2217..>3120)
                     /locus_tag="VLI90_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13619.1"
                     /translation="GTDGTLRLDVPAGWTLAPAQQAFHLSNVGDHARLSFTVTAPSAP
                     AAASIAAVAEVNGARYSNERIEIHYEHVPPILLQPPARLKAVALELAIRGQKVGYIAG
                     AGDSVAEAIEQMGYSVTPLNVDDLTPEKLREFDAVVVGIRAFNVRKDLAAKVPALSAY
                     AESGGTVIEQYNRPDGLQNVALAPYDLRISGDRVTDERAKVTFLAPDHPVLNTPNKIS
                     EADFAGWVQERGIYYPNHWGEEFTPILACCDPGEAPLKGGLLVAKHGKGYFVYTSLVF
                     FRELPAGVPGAYRLFANLLSLGKP"
BASE COUNT          630 a          995 c          964 g          531 t
ORIGIN      
        1 atcgccaggc aaaaatccgc gacgttggcc gcgtcggtct gcttgaacgc ctccacccag
       61 caccgatcca catacgccgg cagctggatc gccgtcttct cgcgccaatc gcgcatcacc
      121 cgctttagat acagcgggga atatttgcgt tgaagtttat tttcaaacgt gaagttgatg
      181 gcgaacgctt cgctgttctt gcgatggcgc aggatcgttt tcgtttcacg cggatcatga
      241 tcgctgtcgt gatagaagaa gacgacctcg ccgccgatct cctgttgcag ccggcgcgcg
      301 gtcgcgatct tcgcgtacag aaatcgcttg gggaaaatgc cgcacggttg ctggccgaag
      361 cagatgatgg gagcgccggc actcatacgt tcgttgtgcc ccgccgaagc atcgcctgca
      421 gcacgatcgc cagcagcatg cacgcggcac aaccgatgat gttgtaccag agatagctga
      481 tcgtcaggtg gtagtagaac agcagcacga gcacttgggc gaccaatgtc gagacgaaga
      541 tggcggtgcc gccgatccat cgcaggaaga acgcgacgag gaacaggccg agcacggggc
      601 cgtagaagac ggacccgatg atgttgccgg cttggatcag gttctccgac agatgcgcga
      661 acagcgcgaa gccgatcgcc accagtcccc agaacacggt gaagcacttg gtggcgaaga
      721 cgaagtgggc gtcgctggcg ctgcgccgga tgacatggcg ataaaaatcc acggtcgtcg
      781 tagaccccag cgcgttcagc tccgccgcct tggaagacat cgccgcggcg aagaacgccg
      841 ccaccagcag gccgatcacg ccgtgcggca gctggtccag gataaacgtg atgaaaacgt
      901 agtcgttgtc gtttgcgttg gtccgattcg ccgccgtcat cagcgacttc gcccgcgcgc
      961 gaacctcatc ggcgcgacgg tccatcgcca acagctcacg cttcgccgaa gcggcggcgg
     1021 cagcgtcgcc atcgcgccga gcccgcagcc acgcgtcgat ctgctcgcgc ttgcgcgcat
     1081 gcgcatcggc aaacgccgct tccatcgcgc gcatcgccgg cgcatcgccg cgattctctt
     1141 ggtccaccca agccgtgcga ttgaagtaca ccggcggcgc ctggaactga tagaagacga
     1201 acagcagcac gcccagcagc aggatgaaga actgcatcgg gattttgaac accgcgttga
     1261 acatcaaccc cagccggctt tcgcgcagcg atgcgccgga gatgtaacgc tggacctgcg
     1321 actgatccgt gccaaagtag gagagcgcca ggaacatccc gccgatcgtg ccggaccaga
     1381 acgtgtagcg ctggtgcagg tcggtgctga acgtcacgcc attaagcttg tgaaatcgcc
     1441 cggcaatcgt cagcgcttca ccaaaatgta aatcatgcgg cagcttcgcc accagcaccg
     1501 caaacgcggt gatcatcccc gcgaagatca cgcccagctg atatttctgc gtgaccgtca
     1561 ccgcgtcagt cccgccggtg acggtgtaga cgatcacgac aagaccggtg cagaggatcg
     1621 tcagatcgag ccgccaaccg aagacagtgg agagcacgat ggccggggcg tagatcgtga
     1681 tgccggcgcc gagtccgcgc tgcagcagga acagcgccgc cccgagcagg cgcgtcttgg
     1741 aatcgaagcg cttgcccaga tattcatatg cggtgttcac cttgagccgg cgatagatcg
     1801 gcagaaacac cgccgcgatg aggatcagcg cgaagggcgc gccgaaataa ttctgcacga
     1861 agccgagacc gtcgttaaat ccctggcccg gcgtggagag gaacgtcgtc gcgctggcct
     1921 gcgtggccat gacggacagg ccgatcacga tccaccgcgc gcgaccatcg cctttgagat
     1981 aggtgttcag gttgcgccgg ccgcgcgtgc gccacatgcc gtaggcggcg atccccagca
     2041 ggctgccgat gagaaccacg tagtcgagcg cgttcacgaa taggttctct cgagcgcgat
     2101 cagcagcgcc acccagatga cgaagacgac gaggacgaac aggtaaaccg ccggccacgt
     2161 gcgcagcgcc ggcagtccgg tgtcgtcgct ttcggtcgat acacccgggt cggcggtcat
     2221 ggcttgccca acgagagcag gtttgcgaag agccgatacg cccccggcac accggcgggt
     2281 agctcgcgga aaaagacgag gctcgtgtaa acgaaatatc ccttgccgtg cttcgcgacc
     2341 aggagcccgc ccttcagcgg cgcttcgcct ggatcacagc acgccaggat cggcgtgaac
     2401 tcctcgcccc aatgattcgg gtaataaatt ccgcgctcct gcacccagcc ggcgaagtcg
     2461 gcttcgctga tcttgttggg cgtattcagc actgggtgat ccggcgccag gaacgtcacc
     2521 tttgcgcgtt catcggtgac ccggtcgccg gaaatgcgca ggtcatatgg cgcgagcgcg
     2581 acgttctgca gtccatccgg ccggttgtac tgctcgatca ccgtgccacc cgattcagca
     2641 tatgctgaca acgccggcac cttcgcggcc aggtctttcc gcacgttgaa cgcgcgaatg
     2701 ccgacgacga cggcgtcgaa ctcgcgcagc ttctcgggcg tcagatcgtc cacattcagc
     2761 ggcgtgaccg agtatcccat ttgctcgatc gcctcggcga cgctgtcgcc agcgccggcg
     2821 atgtatccga ccttttgccc gcgaatggca agctccagcg cgaccgcctt cagccgcgcc
     2881 ggtggctgca gcaagatcgg cgggacgtgc tcgtagtgaa tctcgatccg ttcgttgctg
     2941 tagcgcgcgc cgttcacctc ggcgacggcc gcgatgcttg ccgccgccgg cgcggatggc
     3001 gcggtgaccg taaaactcaa tcgagcatga tcgccaacgt tcgacagatg aaacgcctgc
     3061 tgggccggag cgagcgtcca tcccgcgggg acgtccagcc gcagcgttcc atcggtgccg
//
LOCUS       DATIMH010000301         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2376604, whole genome shotgun sequence.
ACCESSION   DATIMH010000301 DATIMH010000000
VERSION     DATIMH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376604"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            723..>2204
                     /locus_tag="VLI90_05135"
     CDS             723..>2204
                     /locus_tag="VLI90_05135"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HSV13620.1"
                     /translation="MPLPTTPPSGPVSLSQNARTVLAKRYLVKDDKGKPVESPEDLFW
                     RVATVVAEADRRYGASEGDVRAVAEEFYGLMTQRRFEPNSPTLMNAGRPLGQLSACFV
                     LPVEDSLSNGQNGIYDTLRSMAIIHQSGGGTGFSFSRLRQKGAMVRSTTGVASGPISF
                     MKLYDASTDAVKQGGTRRGANMGILRVDHPDILEFIACKEDLTQITNFNISVAVTTDF
                     MNAVKSKSDYALRDTKTGKEVARLDANMVWDKMILGAWRTGEPGCFYIDEANRYNPVP
                     HLGAYEATNPCGEQPLLPYDVCNLGSINVGLYVKDGVMDWDAFRRDIQLSTHLLDNII
                     DVNKYPLPEIDALSKRIRRIGLGLMGFADMLVRLGIPYDSAEGVEMGRRVMEFLDVES
                     EKESERLAKERGAFPEWARSIWGPDETCARDAEGKRIRPMQMLRNCNVTTIAPTGTIS
                     IIAGCSSGLEPLFAVAFMRNQAGVMMPDVNDDFVAIAKKEGWYS"
BASE COUNT          407 a          676 c          752 g          369 t
ORIGIN      
        1 ccgcatcgtg cggccgacga ccgcggcgga acgggagcgg attgcgcgga cggtggacag
       61 ctacctggcg atgcaggagc gtcaccggcg gggggaattt ccgctgcgcc gaaattcctg
      121 atcgcgcacc gcggccggaa ctggtagctt gatgagcggc aacgcggccg gaattcccgc
      181 cagcgcgctc aacgttaggc agcgccggtc tcgaccgtct gcttcgcgca ttccccggtc
      241 acgggcacgg cgcatgcttg acgtgtttgc gggcaagcgg gagttccgcg gccgcgcgat
      301 cgcgagttga ccctcgaagt tcggaacgcg gccggcgcgc cgccgctttc gcccagttgt
      361 tttccaacgc cagcgcaggc atgcacttac gggttgcggc cggaactttc ccggccgcac
      421 tcggcgtttg tctacggtgg cggcgacgat gttacgagcg tcaccagtga tgagcggtgg
      481 aaagatcagg ccggcaggac attcgtgatg ttgataacgt aaagcaaata actgcaaggc
      541 gttgcgagaa cactggcgcg gaatcacgat cgcgactagg ttgcccgccc gcctcggaat
      601 cccgcggttt ttgaaggagc cggcggttcg tccggcagct tgttgaagga ccagatcagc
      661 ttcacctgtg gcccccccgg ccgctgtctt ttttcatcgt ttcactgtac gaggtgttga
      721 agatgcccct ccccaccacc cctccgagcg gtcccgtctc cctcagccag aatgcgcgca
      781 ctgtgttggc caagcgctac ctggtgaagg acgacaaggg aaagccggtg gagagtcccg
      841 aagatctgtt ctggcgcgtg gcgaccgtcg tcgccgaagc tgatcgccgc tacggggcga
      901 gtgagggaga cgtacgggcg gtggccgagg agttctacgg gctgatgacg cagcgccggt
      961 tcgagccgaa ctcgccgacg ctgatgaatg ccggccggcc gttaggccag ctgtcggcgt
     1021 gcttcgtcct ccccgtcgag gattcgctgt cgaacggaca gaacgggatc tacgacacgc
     1081 tgcgctcgat ggcgatcatc caccagagcg gcggcggcac cggcttctcg ttctcgcggc
     1141 tgcgccagaa gggggcgatg gtccgctcga cgaccggcgt cgccagcggc ccgatctcgt
     1201 tcatgaagct gtacgacgcg agcaccgatg cggtgaagca gggtgggacg cgccgcggcg
     1261 cgaacatggg aattctccgc gtcgatcatc cggacatcct cgagttcatc gcctgcaagg
     1321 aagatctcac gcagatcacc aacttcaaca tctcggtcgc ggtgacgacc gatttcatga
     1381 acgcggtgaa gtcaaagagc gattacgcgc tgcgcgacac gaagacgggc aaggaagtcg
     1441 ctcgtctcga cgcgaacatg gtctgggaca agatgatcct cggcgcctgg cgcacggggg
     1501 agccgggctg cttctacatc gacgaggcca atcgctacaa cccggtgccg cacctcggcg
     1561 cctacgaagc gaccaacccg tgcggcgagc agccgctgct gccgtacgac gtctgcaacc
     1621 tcggcagcat caacgtcggg ctgtacgtca aggacggggt gatggactgg gatgcgttcc
     1681 gtcgcgacat ccagctcagc acgcacctgc tcgacaacat catcgacgtc aacaagtacc
     1741 cgctgccgga gatcgacgcg ctgtcgaagc ggatccgccg gatcgggctc gggctgatgg
     1801 gattcgccga catgctcgta cggttaggca tcccgtacga ttccgcggaa ggcgtcgaga
     1861 tgggacgccg cgtgatggag ttcctggacg tcgagagcga gaaggagagc gagcggctgg
     1921 cgaaggagcg cggcgcgttc ccggagtggg cgcgcagcat ctggggaccg gacgagacgt
     1981 gcgcgcgtga tgcggagggg aagcgcatcc gcccgatgca gatgcttcgc aactgcaacg
     2041 tgacgacgat cgcgccgacg gggacgatca gcatcatcgc cggctgctcg agcggtctcg
     2101 agccgctgtt cgcggtcgcg ttcatgcgca accaggcggg cgtgatgatg cccgacgtga
     2161 acgacgactt cgtcgcgatc gcgaagaagg aagggtggta cagc
//
LOCUS       DATIMH010000302         1607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3821076, whole genome shotgun sequence.
ACCESSION   DATIMH010000302 DATIMH010000000
VERSION     DATIMH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3821076"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /gene="flgL"
                     /locus_tag="VLI90_05140"
     CDS             <1..814
                     /gene="flgL"
                     /locus_tag="VLI90_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HSV13621.1"
                     /translation="KDGQGDYIFAGNRTGAAPFVSQTVGVNYVGDDGQRMVAAGPGLQ
                     VATGDPGSAIFKNIPTGNGSFAVTAGASNAGSAVAGASSVSDLAAWDGGSYSVVFTAA
                     DAYEVRDGSGTAISTGSYDPAKGGTVAFKGIQLAISGTPQSGDAFAVAPSGTQDIFST
                     MDNIIATLSQPNGGGAATQNALNTQFANLDQSIDTLTRARAGIGARMNALDQQGSLND
                     DLTLQYKTSLTGVQDLDYYDAISKFSLQQSSLQAAQLTFTKLQSSKLFDYLK"
     gene            996..>1607
                     /locus_tag="VLI90_05145"
     CDS             996..>1607
                     /locus_tag="VLI90_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HSV13622.1"
                     /translation="MSVNTNVSSLNAQKNLATSQSKLSTAIARLSSGMRINSAKDDAA
                     GLAISTRFTTQINGLNQAVSNANDGISLAQTTESALNEVTNNMQRIRTLAVQSANATN
                     SDSDRAALDAEVQQRLSEITRISQQTTFNGRHVLDGTFGNAAFQIGANVGETISVNLS
                     QGAGAKQVGQVATTSTGDVSSMFASPAGLTLAAGDLTVNGTNVA"
BASE COUNT          326 a          571 c          485 g          225 t
ORIGIN      
        1 caaggacggg cagggcgatt acattttcgc cggcaaccgc accggcgcgg caccgttcgt
       61 gtcgcagacg gtgggcgtga attacgtggg cgacgacggc cagcgcatgg tcgccgccgg
      121 gccgggcctg caggtcgcca ccggcgatcc ggggagtgcg atcttcaaga acatccccac
      181 cggcaacggc agcttcgcgg tcaccgcggg cgcatccaac gcaggcagcg cggtagccgg
      241 cgccagcagt gtcagcgatt tagcggcgtg ggacggcggc agctacagcg tggtgttcac
      301 cgcggccgat gcatacgagg tgcgcgacgg ttccggcacg gcgatctcca ccggcagcta
      361 cgatccggcc aagggcggca ccgtcgcctt caagggcatc cagctggcga tcagtggcac
      421 accgcaaagc ggcgatgcgt tcgcggtggc gccgtccggt acgcaggaca tcttcagcac
      481 gatggacaac atcatcgcca cgctcagcca gcccaacggc ggcggcgcgg cgacgcagaa
      541 cgcgctcaac acgcagttcg ccaacctcga ccagtcgatc gacacgctca cccgcgcgcg
      601 cgccggcatc ggcgcgcgca tgaacgcgct cgaccagcaa ggctcgctca acgacgacct
      661 cacgctgcag tacaagacgt cgctgaccgg cgtacaggat ctcgattact acgacgcgat
      721 cagcaagttc agcctgcaac agtcctcgct gcaggccgcg cagctgacct tcaccaagct
      781 gcagtcgtcc aagctgttcg actatctgaa gtgacgcgct ggcgttcgcg ccaggccgca
      841 agaagaaacg taccgatttt ccattgacgc actcaagttt ccatgcaagg ggtcgaaaaa
      901 gtgtctgagg cggcggtgtt cccaacggaa tccccgctgg ctaccaccgc cggccaccgg
      961 cactacagac ccactttgga gaaactccca tggtcatgag cgtcaacacc aacgtcagct
     1021 cgctgaacgc ccagaagaac ctggcgacgt cgcagagcaa gttgtccacc gccatcgcgc
     1081 gcctgtcctc gggcatgcgc atcaacagcg ccaaggacga tgccgccggc ctcgccatct
     1141 cgacgcgctt caccacccag atcaacggcc tgaaccaggc ggtcagcaat gccaacgacg
     1201 gcatctcgct ggcgcagacc accgagtcgg cgttgaacga ggtgaccaac aacatgcagc
     1261 gcatccgcac gctggccgtg cagtccgcca acgccaccaa ctccgacagc gaccgcgccg
     1321 cgctcgatgc cgaagtgcag cagcgtctgt cggaaatcac ccgcatctcg cagcagacca
     1381 ccttcaacgg ccgtcatgtg ctcgatggca cgttcggcaa cgccgcgttc cagatcggcg
     1441 ccaacgtcgg cgaaaccatc tcggtgaacc tgagccaggg cgccggcgcc aagcaggttg
     1501 gccaggtcgc caccacttcg accggcgacg tcagcagcat gttcgccagc ccggccggct
     1561 tgaccctggc cgcgggcgac ctgaccgtca acggcaccaa cgtcgcc
//
LOCUS       DATIMH010000303         1566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2376685, whole genome shotgun sequence.
ACCESSION   DATIMH010000303 DATIMH010000000
VERSION     DATIMH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376685"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VLI90_05150"
     CDS             <1..394
                     /locus_tag="VLI90_05150"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HSV13623.1"
                     /translation="SLRSTTSGGLGYYIVKTEKNSIDLRAGPAFVWEKLFNGETHADA
                     SGLAGLRAVHTINDHVNLSQEILYTVAFSDTNRYQITGETALNVKLPEVARGMGLKLA
                     FRDDYDNGTSAPRKNNDTRLTLAMTLDF"
     gene            complement(539..781)
                     /locus_tag="VLI90_05155"
     CDS             complement(539..781)
                     /locus_tag="VLI90_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HSV13624.1"
                     /translation="MSEPNATQTATINIQGMTCQHCTQSVQRAVSSLPGVSAVNVDLA
                     KNAATVTFNPASVTVGKVMQTIQQAGFTPVGFTKSA"
     gene            complement(789..>1566)
                     /locus_tag="VLI90_05160"
     CDS             complement(789..>1566)
                     /locus_tag="VLI90_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV13625.1"
                     /translation="RIPRDLPAFLQDIFRQPLMPHEVETDAFRQMNYLNYRISRLQRS
                     LDVETASAEQLTEIESLLAQSAALKNLIVQSNLRVAVHVARKHQRVDRPLMELVSDAT
                     IWLMRAADKFDFARAGGGGSGARFSTYASFALMKNFARDRVEQLTRRDSHLLTGQEEI
                     INTLGDRETDAAGERIDAANLQTDLLTVIQELPPRERELLTHHYGLDQTKPALSLAQI
                     GDKMGITKARVRQLEARALRKLRHLLESRREKLHKATARK"
BASE COUNT          275 a          481 c          494 g          316 t
ORIGIN      
        1 ttcgctgcgt tcgacaacct ccggcggtct gggctattac atcgtcaaaa ctgaaaagaa
       61 cagcatcgac ctccgcgccg gcccggcctt cgtctgggag aaattgttca acggcgaaac
      121 ccacgcagac gcctccggcc tggccgggct tcgtgccgtt cacaccatca atgaccacgt
      181 gaatctgtct caggagattc tgtacacggt cgccttttcc gataccaacc gctatcagat
      241 caccggcgag acggccctca acgtgaagct ccccgaagtc gcccgcggca tgggcctcaa
      301 gctcgccttc cgcgatgact acgacaacgg cacctccgcc ccgcgcaaga acaatgacac
      361 ccgcctgacg ctggcgatga cgctggattt ctaaggcgct ggagttcccg tcagcccccg
      421 gctcggccgg gggtacgcgc gcggtgggcg attatttatg agaggggacc gcgcagtcgc
      481 tgttcttcac gttcgcaccc ccggccgagc cgggggctga cggctgcgtg gccgatcgct
      541 acgcggattt ggtaaatccc acgggcgtga agccggcttg ctggatggtc tgcataacct
      601 tgccgacggt gacggaggca gggttgaacg tgaccgtcgc ggcgtttttg gcgaggtcga
      661 cattaacggc gctcactccc ggcaggctcg agacggcccg ctgaacactc tgcgtgcagt
      721 gctggcaggt cattccctgg atgttaatcg tggcggtttg cgtggcgttg ggttcactca
      781 tggcgggttc actttcttgc ggtggccttg tgaagtttct cgcgccggga ttccagcagg
      841 tggcggagtt tgcgaagcgc ccgggcctca agctgccgga cgcgggcctt ggtgataccc
      901 atcttgtcgc cgatctgcgc cagcgagagg gcgggcttgg tctggtcgag gccgtagtga
      961 tgggtcaaca gttcgcgctc gcgcgggggc agttcctgga tcaccgtcaa caaatccgtt
     1021 tgtagattgg cggcgtcaat tcgttctcca gcagcgtcgg tttcgcggtc gcccagggtg
     1081 ttgatgattt cttcctgccc cgtgagcagg tgagagtcgc gacgcgtaag ctgctcgacg
     1141 cggtcgcggg cgaagttttt catcagggca aaactggcgt acgtggaaaa acgcgccccg
     1201 ctcccgccgc ccccggcccg cgcgaaatca aatttgtccg cggcgcgcat cagccagatc
     1261 gtggcatccg acaccagttc catcagcggg cgatcgacgc gctggtgctt gcgcgccaca
     1321 tgcacggcca cgcggagatt gctctgcacg atcagattct tcaacgccgc ggactgggcc
     1381 agcagcgact cgatctccgt gagttgttcc gccgacgccg tttccacatc aagcgaccgc
     1441 tgcaaacggc tgatgcggta attgagataa ttcatctgtc gaaatgcgtc ggtttccact
     1501 tcgtggggca taagcggctg gcggaaaatg tcctgaagaa acgccggcag atcgcgcggg
     1561 atgcgg
//
LOCUS       DATIMH010000304         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4978612, whole genome shotgun sequence.
ACCESSION   DATIMH010000304 DATIMH010000000
VERSION     DATIMH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4978612"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VLI90_05165"
     CDS             complement(<1..440)
                     /locus_tag="VLI90_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV13626.1"
                     /translation="MDQRRILLLITDLELGGTPTVVRELAVRLRRDVAAAHVEVACLS
                     KWGPVATQLQEAGVTVTALDAKGVADLGIVARLIRFLHGRKFDTVFSFLVHANTVAAM
                     ASVSVRDIRWIQAIQTTQPWPRWHWLAQGVAQLAADAVVVPSPSV"
     gene            complement(547..957)
                     /locus_tag="VLI90_05170"
     CDS             complement(547..957)
                     /locus_tag="VLI90_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13627.1"
                     /translation="MRSSHFSSGGRYPVLRSLAIIYMIAAILTGIGGIVLACWIFFSL
                     RGMENHITSGILVLAGTFLVVLSMLAVAEVLKLFIDIEHNSRMMYYGRERVSEVTTAD
                     EAVATTAGPNGARMTGKRWLEGEETAEGALLRGH"
     gene            complement(1044..1874)
                     /locus_tag="VLI90_05175"
     CDS             complement(1044..1874)
                     /locus_tag="VLI90_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSV13628.1"
                     /translation="MLKRPRRLALLVLIPLIVIAAVAWWVTLPVSTGPAEGSSFPGIA
                     ATQPVVPSTLRIATFNIDGGRGTDGVVDLERTARSLQRIDFVGLNEVHNDLFGSQQNQ
                     AASLAAPLHTPGLFVPSERRLGRETFGNAVFTRLPVKHWQRIVLPSGPFKAPRNYLLT
                     DVDWHGVTVHFLTTHTDWKSGGAEQRQIVADAFLNLPEPAVLMGDLNAPQHDPLIERL
                     LASKGVEEAIDAILGPEPGRVDWIFLRGLTALDAGRVDIGASDHPAYWAQVRLRSEIR
                     "
     gene            complement(1904..>2805)
                     /locus_tag="VLI90_05180"
     CDS             complement(1904..>2805)
                     /locus_tag="VLI90_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13629.1"
                     /translation="ILSAGCGSPPVKHPPPQDPDLEVFNATARLEFDRGSLGPAAASY
                     ARALDRARMMDDAAAIATAAYNLAVCRAALGDYPAARMLLDEADREARRSNLSTADVY
                     LALARIALLEHDPTATARLSDAVLNPEARPDTEQRAQAHVLHGLAACDLSDAGLAKQQ
                     LDAARSLAATVKDPALDASVWALSAAVHLLAHDWAAAAADFDREADLRRTMHQYRELE
                     RALTKAGRAFAAVGDHREAADRLYRAARAAAARGGADAPALASEANTAARKAGDESLV
                     RLTASLQQSLTVATTPATRPAID"
BASE COUNT          527 a          897 c          922 g          459 t
ORIGIN      
        1 accgatggtg agggaacgac cacagcatca gcggccagct gggcgacgcc ctgcgcgagc
       61 caatgccacc gcggccacgg ttgggttgtc tgaattgcct gaatccaccg aatgtcgcgc
      121 accgacaccg atgccattgc agcgacggta ttcgcgtgaa cgaggaagct gaagacggta
      181 tcgaatttcc gtccgtgaag gaagcggatg agccgggcga cgatgccgag gtctgccaca
      241 cctttggcat cgagggcggt gacggtcacg cccgcttctt gaagctgcgt cgcaaccggt
      301 ccccatttgg acaggcaagc cacttccacg tgcgcggccg cgacatcgcg gcgcaagcgc
      361 acggcgagtt cgcgcacgac cgtaggcgtg ccaccgagtt cgaggtcggt gatgagcagc
      421 aggatgcgac gctgatccac gccgctaatg tacggcgggg atcagctttt cgcattggtg
      481 cgcagcgaac accgacaggc cgacctgctt aagcgggtcg ggccggtgtt gcccgcgacg
      541 agccgttcaa tgtccgcgaa gcagcgcgcc ttcggcggtc tcttcgcctt cgagccagcg
      601 cttgccggtc atgcgggcgc cgttcggtcc cgccgtcgtc gccaccgctt cgtcggccgt
      661 cgtcacttcg ctgacgcgct ctcggccgta atacatcatg cgggagttgt gctcgatgtc
      721 gatgaatagc ttgagcacct ccgcgaccgc cagcatgctc agcacaacga ggaacgtccc
      781 cgccagcacc aggatgcccg acgtgatgtg gttctccatc ccgcgtaggg agaagaagat
      841 ccagcatgcc agcacaatcc caccgatgcc ggtgaggatg gcggcaatca tgtaaatgat
      901 ggccaatgag cggagcaccg gatatcggcc gccgcttgaa aaatgcgaac tacgcatgcg
      961 aacctccgat tcagaggacc acgagatatg tcaaaacctg tagagcagta tgagcggggg
     1021 cgtgaagatg gggaacggaa aatttacctg atttccgatc ggagccgaac ctgagcccag
     1081 tatgcgggat ggtcggatgc accgatatcc acgcgaccag cgtcaagcgc ggtcagcccg
     1141 cgcaggaaaa tccagtcaac ccgccccggt tcggggccca ggatcgcatc aatggcttct
     1201 tcgacgccct tgctggcgag cagccgctcg atcagcgggt cgtgctgcgg cgcgttcaaa
     1261 tcgcccatca gcaccgccgg ttcgggcaga ttgagaaacg catcggccac gatctgccgc
     1321 tgctccgccc caccggattt ccaatcggtg tgcgtcgtca gaaagtggac ggtcacgccg
     1381 tgccaatcga cgtcggtgag caggtagttg cgcggcgctt tgaacggacc gctcggcagc
     1441 acgatgcgtt gccaatgttt gacaggcaat cgcgtgaaga cggcgttgcc gaacgtctct
     1501 cgccccagtc ggcgctcgga cggcacaaac aaccccggcg tgtgcagcgg cgccgccagc
     1561 gaggccgcct ggttctgttg gctgccgaac aaatcgttgt gcacctcgtt tagaccgacg
     1621 aaatcaatac gctgcagcga tcgcgccgtg cgctcgagat cgacgacgcc gtccgtcccg
     1681 cggccaccgt cgatgttgaa ggtggcgatg cgcagcgtcg aaggcacgac cggctgcgtg
     1741 gctgcgatgc ctgggaacga cgagccctca gccggaccgg tggaaaccgg cagcgtcacc
     1801 caccacgcaa ccgccgcgat gacgatgagc gggatgagaa ccagcagcgc caaccggcgg
     1861 ggacgtttga gcatcggcgg gaacgtaccg ggagcagatc ggctcagtca attgccgggc
     1921 gcgttgcggg tgtggtggcc accgtcaacg actgctgcag ggatgcggta aggcgcacga
     1981 gcgattcatc gcccgctttg cgagcggccg tattggcttc actggcgagg gccggcgcgt
     2041 cggccccacc gcgcgcagcc gccgcgcggg cggcacgata cagacgatcg gccgcctcac
     2101 gatgatcgcc cacggcggca aatgcgcgtc cggctttggt cagcgcccgt tccagttccc
     2161 gatattggtg catcgtccgg cgcaggtccg cttcgcgatc gaaatccgcc gcagcagcag
     2221 cccagtcgtg cgccaacaga tgaaccgcgg cagacagcgc ccagacggat gcgtcaagcg
     2281 ccgggtcttt cacggtggca gcgagtgacc tggcagcgtc caattgctgc ttcgcaaggc
     2341 cggcgtcgct cagatcacac gctgccaatc cgtgcaggac atgcgcctgc gcgcgttgct
     2401 cggtgtccgg ccgagcttcg ggattgagca cggcatcaga gagcctggcg gtcgcggtgg
     2461 gatcgtgctc caacagcgcg atccgcgcca gggcgagata gacatccgcg gtgctgaggt
     2521 tcgaccgtcg tgcttcgcgg tcggcttcat cgagcaacat ccgagccgcg ggataatcgc
     2581 ccagcgccgc ccggcagacg gccaggttgt acgcggcggt ggcaatcgcg gcggcgtcgt
     2641 ccatcatgcg ggcacggtcc aatgcccgcg cataagatgc cgcggcgggg ccgagcgagc
     2701 cgcggtcgaa ctccagtcgt gccgtcgcat tgaatacttc cagatccgga tcctgcggcg
     2761 gcgggtgttt caccggcggt gagccgcaac ccgccgataa gatca
//
LOCUS       DATIMH010000305         8869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1225398, whole genome shotgun sequence.
ACCESSION   DATIMH010000305 DATIMH010000000
VERSION     DATIMH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225398"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VLI90_05185"
     CDS             complement(<1..782)
                     /locus_tag="VLI90_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV13630.1"
                     /translation="MQSSAPTLPRRHDHRIGVLGSGFIVNDCHLVSYRKYGLNPVAIA
                     SRTRANAERVARHHEIAKVHEDYDALLDDASIEVLDIAVTPDVQLDLIRRACRRRTLK
                     GILAQKPLGMNLAEAREAVRLCDEAGIKLAVNQNMRHDPSVRAVHSLLAAGALGTPVF
                     ASIDMRAIPHFQPWQQQLGWVTLRIMSIHHLDCLRHWFGDPQRIFCSTRSDPRTTFPH
                     TDGICTYTLEYADGLRCVGIDDIWTGPAREGCPADIGIGYRI"
     gene            complement(786..3101)
                     /gene="nqrF"
                     /locus_tag="VLI90_05190"
     CDS             complement(786..3101)
                     /gene="nqrF"
                     /locus_tag="VLI90_05190"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HSV13631.1"
                     /translation="MEFDNDHGPAVLTRPPKHLDYSLTGEETGRAIERGLAEAEWYQC
                     PVPRATMRELLDRRNGPAIRDTIIWFTLIIGLGALTWWLWPSWWAALPYLAYATLYAS
                     TSDSRWHEAGHGTAFKTDWMNNALYEIASFMVMRESTLWRWSHTRHHSDTIIVGRDPE
                     IAVPRPPNLRAMLLTVVGLQLYPPYFKAIVRHTLGRMSGAERTFIPESEFGKIYFRAR
                     IYVLIYLSVIVLAICTRSILPLLLVGGTHIFGTWLMLIYGWTQHAGLAENVLDHRLNC
                     RTVYMNPIHRFLYWNMNYHVEHHMFPLVPYHALPRLHALVKDDCPTPYPSIFAAWREI
                     VPTVLRQRKDPSYYVRRKLPETKPAVAVSLQQLDGRWPDVTPDAEGWVEVCAAADLDV
                     ADVIRFDLGRRTFALYRDETGNLFATDGICTHGNTHLADGLVKDGQIECPKHNGRFNL
                     NDGSPARAPVCRGLATYPIEERAGRLWLNVVRAGGAGARSQKTVNLRVISNRNVATFI
                     RELVLQPVDGQNAVEFRPGDYLQIDIPQYEEIRFRDLDVPEPYRSVWERQHVFDLVAR
                     NPSCSARRNNYSIASNAVTEPGVLRFNVRIATPPPGQDCPPGVGSTYVWNLRPGDTVS
                     AIGPFGDFHIKPTQREMVYIGGGAGMAPMRAHLSHLFETEPTQRRVSYWYGARSRQEL
                     FYEDYFAGLAATHANFRFELALSSPLPEDNWTGHGGLINEVVLEQYLSQHPNPRAIEY
                     YLCGPPMMVKACMKMLAELGVPANHISYDEF"
     gene            complement(3111..4163)
                     /locus_tag="VLI90_05195"
     CDS             complement(3111..4163)
                     /locus_tag="VLI90_05195"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HSV13632.1"
                     /translation="MAEKLESRNCGHLPGGETVDAFVLEGAGGVALEAITLGGIVTRL
                     LVPDQRGNRADVVLGFADLAGYLRPHPYFGAIAGRVAGRISGSRFTIDGQTHSLSCNE
                     PPNHLHGGARGFDKRLWSAQPVARADGAPSLRLSRISPNGEEGYPGNLRVSVTYAITA
                     RNEFIIDSEATTDRPTPISLTHHSYFNLSGEGSSSVAHHKLQIMASRYAPTDSAFAFI
                     GRRDAVDGNDFRAARRMSDAIPHLYGQHGDLYFIDRPAPSPATLVDAAQLIDPSSGRV
                     MTVRTTEACIQLYTGTGLDGSIVGKSGRPYGPFAGMCLECEGFPDGANRPDLGNVILR
                     PGGVFRQTTVYAFSTL"
     gene            4291..4638
                     /locus_tag="VLI90_05200"
     CDS             4291..4638
                     /locus_tag="VLI90_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HSV13633.1"
                     /translation="MIAIPRCFKVDAADNVATMLDDAEPGAARVIGAAVDDAAAAALV
                     LREAIQLGHKVALTDIPAGEAVVKFGVPIGRASSQIRRGDWVHLHNCESNFDARSGSL
                     DLHSGAATDTKYE"
     gene            4747..5973
                     /locus_tag="VLI90_05205"
     CDS             4747..5973
                     /locus_tag="VLI90_05205"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HSV13634.1"
                     /translation="MGNGVTTSNTGPVWQGYLRSDGRRGIRNLVLVVHTVECAKFVAE
                     AIAKDEPDVHVIGFSGCYDNQYAIRLMLALARHPNVGAVLAVGLGCEYTQPEKIAEVV
                     RESGRPAEWFYIQDAGGTRTGVERGKQLVAKLREQIANTPRVEMRVSDLTIGCECGGS
                     DGTSGLAGNPVVGAFFDQLVDSGGRAIFEEIVEMIGLKQIMLARAASPMAREQIAAAY
                     DKAVRYCQDVRQYSVSPGNFAGGLTTIEEKSMGAFAKSGTRAIQGVIKVAQSPPAPGL
                     WLMDSVPDPHFMGFGYTNPNDTEGIMDLISGGCQIVLFVTGRGSVIGSPVAPLIKVTG
                     NSQTYRRMQDDMDFDAGRVLTGERTLLEAATELRDLIARVAAGEPSKPEALGHREYFV
                     MYKHQDTPSLEAGCRA"
     gene            5970..6737
                     /locus_tag="VLI90_05210"
     CDS             5970..6737
                     /locus_tag="VLI90_05210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV13635.1"
                     /translation="MSDFVNAFTLDGEVALITGGGSGLGLGMARCMAQAGARVVLVGR
                     RRSVLDDACASIGERAVAEPYDVTQFDQADAMVQRVTERVGSITILANNAGIHLKRPA
                     VETTIDGFNSVLQTHVLAAHNLTRAVLPGMISRKHGSVLFMASMASLMGLPQVIAYSA
                     AKSAYVGMVRTLSTEVAQHGVRVNAIAPGWIESDMLHKALDGDPPRKAKILARTSMAR
                     FGVPDDIGWAAVYLCSPAAKFVTGVVLPVDGGASSGF"
     gene            complement(6890..8182)
                     /locus_tag="VLI90_05215"
     CDS             complement(6890..8182)
                     /locus_tag="VLI90_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV13636.1"
                     /translation="MTASDQLNPIASDPPGGAGVGGLRWWICALLFFATTINYVDRQV
                     ISVLKPTLQKQFSWNEIDYSNIVFWFQVAYAGGYLFAGRLMDRVGLRIGYTIAIVLWS
                     VAAMSHAFARTAAQFAAARFGLGLAEGGNFPAAIKTVSEWFPKKERALATGLFNAGSN
                     VGAIIAPLIVPWITVRYGWPWAFGVTGSLGLLCVVAWLVLYRRPEMHPGLAPSELAYI
                     RSDPPDPTVHVPWAQLLHYRATWAFIVGMAMSSPIWWFYLYWVPGFLFDRYGVDLMHM
                     GPPLVVIYVISDFGSILGGWVSSRLIQRGWNVLTARKVTLLACALCVVPVFFASRVSS
                     VWPATLLIALAAAGHQGWAANLYTLVSDAMPRHTVSSVVGIGGFAGAVAGMFFAKFVG
                     YVLQWTQSYVLIFALAPAAYLVALSVMHVLLRRSSSLS"
     gene            8345..>8869
                     /locus_tag="VLI90_05220"
     CDS             8345..>8869
                     /locus_tag="VLI90_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HSV13637.1"
                     /translation="MPAAEWTNDAELFQLIRDVLYTPVVGDILDDLGFVHQFLPPPIQ
                     PLREEMKLAGRAMPVLMIDVFGKQKQPFGKLTEALDQLQPGEVYLATGGEMRCAYWGE
                     ILTATAKKRGAVGAIINGFHRDTPKVLEQDWPVFSRGRFAQDSGVRTQVADYRCPIEV
                     GHVHVEPSDLIFGDL"
BASE COUNT         1654 a         2757 c         2901 g         1557 t
ORIGIN      
        1 tcgatgcggt agccgatgcc gatgtcggcc gggcacccct cgcgcgcggg gccggtccag
       61 atgtcgtcga tgccgacgca gcgcaggccg tccgcgtact ccagcgtgta ggtgcagatg
      121 ccgtcggtgt gtggaaacgt cgtgcgcggg tcgctgcgcg tgctgcagaa gatgcgctgc
      181 ggatcgccga accagtgccg caggcaatcg agatgatgga tcgacatgat gcgcagcgtg
      241 acccaaccca gctgctgctg ccacggctga aagtgcggga tggcccgcat gtcgatgctg
      301 gcgaagacgg gtgtgcccag cgcgccggcg gcgagcagcg aatgtaccgc gcgcaccgat
      361 gggtcgtgcc gcatgttctg gttgacggcc agcttgatgc ccgcctcatc gcagaggcgc
      421 accgcttcgc gcgcttccgc caaattcatc cccagcggct tttgcgccag gatgcccttg
      481 agcgtccgcc gccggcacgc ccggcggatc agatccagct gcacatccgg cgtcaccgcg
      541 atgtcgagca cctcgatcga cgcatcatca agcagcgcat cgtaatcttc gtgcaccttg
      601 gcaatctcat gatgcctcgc cacgcgctcg gcattggcgc gcgtgcgcga cgcgatggcg
      661 accggattga gcccgtactt gcgatagctg acgaggtggc agtcattgac gatgaacccg
      721 ctgccgagca ctccaatgcg gtgatcgtgc cggcgtggaa gagtgggggc gctggattgc
      781 atttgttaga actcgtcgta cgagatgtga ttcgccggca cgccgagttc ggcgagcatt
      841 ttcatgcagg ccttcaccat catcggcggg ccgcacaggt aatactcgat cgcccgggga
      901 ttcgggtgtt ggctgagata ttgctcgagc acgacttcat taatcaaacc gccgtggccg
      961 gtccagttgt cctccggcag cggtgacgac agggccaact cgaaccggaa attcgcatgc
     1021 gtggccgcca ggccggcgaa gtagtcttcg tagaacagct cctgccgcga ccgcgcgccg
     1081 taccagtagc tgaccctgcg ttgcgtcggc tcggtctcga acagatgcga caggtgcgcc
     1141 cgcatcggcg ccatgccggc gccaccgccg atgtaaacca tctcgcgctg ggtgggcttg
     1201 atgtggaaat ccccgaaggg gccgatggcg gaaaccgtgt cacccggtct caagttccag
     1261 acgtacgtcg atcccacgcc cggcggacaa tcctgtcccg gtggtggcgt ggcgatccgc
     1321 acgttgaatc gcagcacgcc cggttccgtc acggcgttgc tcgcgatcga gtaattgttg
     1381 cgccgcgcgg aacaggatgg gtttcgcgcg accagatcga acacgtgctg ccgttcccac
     1441 acggaccgat acggctcggg cacgtccagg tcgcggaagc gaatctcctc gtactgcggg
     1501 atgtcgatct gcaggtaatc cccggggcgg aactcgaccg cgttctgccc atccaccggc
     1561 tgcaggacca gctcgcgaat gaacgttgcc acgttgcggt tgctgatcac gcggaggttg
     1621 accgtcttct gtgaacgcgc gccagcgccg cccgcgcgca cgacgttcag ccacaaccgg
     1681 ccggcgcgct cttcgatcgg atacgtcgcc aacccgcggc agacgggcgc tcgcgcgggc
     1741 gagccgtcgt tcagattgaa ccggccgttg tgcttcgggc attcgatctg cccgtccttc
     1801 accagcccgt cagccaggtg cgtgttgccg tgcgtgcaga tgccgtcggt ggcgaagagg
     1861 ttgccggttt cgtcgcgata gagcgcgaac gtccgccggc ccaggtcgaa gcggatgacg
     1921 tcggccacat ccagatcggc ggcggcgcag acttcaaccc aaccctcggc atccggtgtg
     1981 acgtccggcc atcggccatc caactgctgc aacgacaccg cgacagcagg ttttgtctcg
     2041 ggcagcttgc gtcgaacgta atacgacgga tctttccgct ggcgcaggac cgttggcacg
     2101 atctcccgcc acgccgcgaa aatgctggga tacggcgtcg ggcagtcgtc tttgacaagg
     2161 gcatgcaacc gcggcagcgc gtggtacggc accagcggga acatgtgatg ttccacgtgg
     2221 tagttcatgt tccagtacag gaaccggtgg atcgggttca tgtacacggt ccggcaattc
     2281 aggcgatgat cgagcacgtt ctcggccagg cccgcgtgct gcgtccagcc gtagatcagc
     2341 atcaaccagg tgccgaagat gtgcgtcccg ccgaccagca gcagcggcag gatgctgcgc
     2401 gtgcaaatcg cgaggacgat caccgacagg taaatcagca cgtagatccg cgcgcggaaa
     2461 tagatcttgc cgaattcact ttcggggatg aacgtgcgct ccgcgccgga cattcggcca
     2521 agcgtgtgcc gcacgatcgc cttgaaatat ggtgggtaca gctgcaggcc gacgaccgtg
     2581 agcaacatcg cccgcaggtt cggcgggcgc ggcacggcga tttccggatc gcggccgacg
     2641 atgatcgtgt cgctgtgatg gcgcgtgtgg ctccatcgcc acagcgtcga ttcgcgcatc
     2701 accatgaatg atgcgatttc atacagcgcg ttgttcatcc agtccgtttt gaaggccgtg
     2761 ccgtgaccgg cttcgtgcca gcgagaatcg gacgtcgatg catagagcgt cgcgtacgcc
     2821 aggtacggca gcgccgccca ccacgacggc cagagccacc acgtcagcgc gccaaggcca
     2881 atgatgagcg tgaaccagat gatcgtgtcg cggatggccg ggccgttccg gcggtcgagc
     2941 aattcgcgca tcgtcgcgcg gggcaccggg cactgatacc actccgcctc ggcaagtccg
     3001 cgctcgatcg cccggccggt ctcttcgccg gtcaggctgt agtcgagatg cttcggtggc
     3061 cgcgtcagga ccgccgggcc gtgatcgttg tcgaattcca tgaccgttcc tcacagcgtc
     3121 gagaacgcgt agaccgtcgt ctgtcgaaaa acaccgcccg gtcgaagaat gacgttgccc
     3181 aggtcggggc ggttcgcgcc gtcgggaaag ccttcgcact cgaggcacat gccggcgaac
     3241 gggccgtagg gcctgccgga tttgccgacg atggatccat cgaggccggt tccagtatat
     3301 agctgaatgc aggcttcggt ggtgcgcacc gtcatgacgc ggccgctgct gggatcgatc
     3361 aattgcgccg catcgacgag cgtggcaggc gatggcgcgg ggcgatcgat gaagtagagg
     3421 tcgccgtgct gtccgtacag gtgcgggatg gcatctgaca tgcgccgggc ggcgcggaag
     3481 tcattgccat cgacggcgtc gcgccggccg atgaaggcga atgccgaatc cgtcggtgcg
     3541 tagcggctgg ccatgatctg cagcttgtga tgcgcgacgg aactcgaccc ttcacccgac
     3601 aggttgaagt acgaatgatg cgtcaggctg atcggcgtcg gccgatcggt cgtcgcttcg
     3661 ctgtcgatga tgaattcgtt ccgggcggtg atggcgtacg tgacgctgac ccgcagattg
     3721 ccgggataac cttcttcacc gttgggactg atgcgcgaca gccgcaagga tggggcacca
     3781 tccgcccgcg cgacaggctg agccgaccag agccgcttgt cgaatccgcg cgcgccgccg
     3841 tgcaggtgat tgggcggctc gttgcagctg agcgaatgag tctggccgtc gatcgtgaag
     3901 cgcgagccgc tgatccgccc ggcgacgcgg ccggcgatgg cgccgaaata cgggtgcggg
     3961 cggaggtagc cagccaaatc ggcaaagccg agcaccacat cggcgcggtt gccgcgctgg
     4021 tctggcacca gcaatcgggt gacgatgccg ccgagcgtga tcgcttcaag cgcaacaccg
     4081 ccggcgcctt cgagcacgaa ggcatccacg gtttcgccgc cgggaagatg gccgcaattg
     4141 cgggactcca gcttttccgc catttcgctg gctgttttta gcagaaatgg gcgacgaggg
     4201 gctttcactt cctgccgtcc gttttggaga atttgacgaa cgtgcgaggg gtgcggcgga
     4261 cacggtatgc ttatcgggcg cgagacgccg atgatagcga ttcccaggtg cttcaaggtc
     4321 gatgcggcgg acaacgtcgc gacgatgctc gacgatgccg agcccggcgc ggcacgcgtg
     4381 attggcgctg cggttgacga tgcggcggcg gcggctctag tccttcgcga ggcgattcaa
     4441 ctgggtcaca aggtggcact gacggacatc cccgcgggcg aggccgttgt gaagttcggc
     4501 gtgccgatcg gccgggcgtc atcgcagatc cgccgcggag attgggtcca cctgcacaac
     4561 tgcgaaagca actttgatgc ccgatctggt agcctggatc tgcacagcgg agcggcgact
     4621 gatacaaaat atgagtgagt aaagaggagc cacagatgca cacagatgca cacggataaa
     4681 aattccatcc gttttatctg tgttcatctg tgtgcatctg tggttaataa gatctttcga
     4741 gtctctatgg gaaacggggt cacaaccagc aacaccggac ctgtttggca gggttatctc
     4801 cgcagcgatg gccgcagggg tattcgcaat cttgtgctgg tcgtgcacac ggtggagtgc
     4861 gcgaagttcg tcgccgaggc catcgccaag gatgagccgg acgtgcacgt gatcgggttt
     4921 tccgggtgct atgacaatca gtatgcgatt cgcctcatgc tggcgctggc gcgccatccg
     4981 aacgttggtg ccgtactggc cgttgggctg ggatgcgagt acacgcagcc ggaaaagatc
     5041 gcggaggtcg tacgagaatc cggtcggccg gccgagtggt tctatattca ggacgccggt
     5101 ggcacgcgca ccggggttga acgagggaag caactggtgg cgaagctgcg cgagcagatt
     5161 gcgaatacgc cgcgcgtcga gatgagagtg tccgatctga cgatcggctg cgagtgcggc
     5221 gggtccgacg gcaccagtgg ccttgctggc aaccccgtcg tcggcgcgtt cttcgatcaa
     5281 ctggttgact ccggtggccg cgcaatcttc gaagagatcg tcgagatgat tggtttgaag
     5341 cagattatgc tcgcgcgggc ggcgtcgccg atggctcgcg agcagatcgc cgcggcgtat
     5401 gacaaggccg tgcgctattg ccaggacgtc cggcagtatt ccgtgtcacc cggcaacttc
     5461 gccggtgggt tgacgacgat cgaagaaaaa agcatgggcg cgttcgccaa gagcggcaca
     5521 cgcgcgattc aaggcgtgat caaggtcgcc caatcaccac cggcgccggg gctttggttg
     5581 atggacagcg tgcccgaccc gcatttcatg ggcttcggtt acacgaaccc caacgacacc
     5641 gaaggcatca tggacctgat cagtggcgga tgccagatcg ttctattcgt caccggccga
     5701 ggcagcgtga tcggctcgcc ggtggcgccg ctgatcaagg tcaccggcaa tagccagacg
     5761 tatcggcgga tgcaggacga catggatttc gacgccggcc gggtgttgac gggcgagcgc
     5821 acgcttttgg aagccgcaac ggaactgcgc gatctgatcg cgcgcgtcgc cgcgggcgaa
     5881 ccaagcaaac cggaggcgct gggccaccgg gagtatttcg tgatgtacaa acaccaggac
     5941 acaccgagcc tggaagcagg gtgccgggca tgagcgattt cgtaaacgca ttcactcttg
     6001 atggcgaagt cgccctgatc accggcgggg gctcggggct gggtctgggc atggcgcgct
     6061 gcatggccca ggcgggcgcg cgcgtcgttc tcgtgggacg tcgcagatca gtgctcgatg
     6121 acgcgtgcgc ctcgatcggc gagcgagccg tcgcggagcc atacgatgtg acacagttcg
     6181 accaggccga tgcgatggtg cagcgcgtca ccgagcgcgt cgggtcgatc acgatcctcg
     6241 cgaacaacgc cggcattcac ctgaagcgcc cggcggtcga gacgacgatc gacggtttca
     6301 attcggtgtt gcagacgcat gtgctggccg cgcacaacct cacccgcgcg gtgctgccgg
     6361 gaatgatttc gcgcaagcac ggcagtgtgc tgttcatggc gtcgatggcc tcgctcatgg
     6421 gattgccgca ggtcatcgca tactccgccg ccaagagcgc ctatgtgggg atggtccgca
     6481 cgctatccac cgaagtcgcc cagcacggcg tgcgcgtgaa tgccatcgcg cccggctgga
     6541 tcgaaagcga catgctgcac aaagcgctgg acggcgaccc gccgcgaaag gcgaagattt
     6601 tggcgcgcac gtcgatggcg agattcggcg tgccggacga catcggctgg gccgccgtgt
     6661 atctctgttc ccctgcggcc aagttcgtga ccggcgtcgt tcttcccgta gacggcggcg
     6721 cgagcagcgg gttctgagat tccgcgatgg tatgaccgcc gcttgcggct tggcagaccc
     6781 ggcggctccg atctgcgaaa ccgcaagcga ctgctgagcc tttcgcgcag ctgaaggttc
     6841 accgactttc cggctagatc cttcgactcc ggcggcgtac cgcctgcgct caggataaac
     6901 tgcttgaccg cctcaacagc acgtgcatca cgctgagcgc caccaaatac gccgccggcg
     6961 cgagcgcgaa gatcagcacg tagctctgcg tccactgcag cacgtaaccg acgaacttcg
     7021 cgaagaacat ccccgccacc gcgccggcga agccgccgat gccgacgacg gagctgacgg
     7081 tgtgccgcgg catcgcatcc gacaccaggg tgtacagatt tgccgcccaa ccctgatgcc
     7141 ccgcggccgc caacgcgatc agcaacgtcg ccggccagac gctggagacg cggctggcga
     7201 agaagaccgg caccacgcac agggcgcacg cgagcagcgt gactttgcgc gcggtaagca
     7261 cgttccaacc acgctggatc aatcgcgatg atacccatcc gccgaggatg ctgccgaaat
     7321 cggaaatgac gtagatcacc accagcggtg ggcccatgtg catcaggtcc acaccgtatc
     7381 gatcgaacaa aaaccccggc acccaataca ggtagaacca ccagatcggg ctgctcatgg
     7441 ccatgccgac gatgaacgcc caggtcgccc ggtagtgcag cagctgggcc cacggcacgt
     7501 gaacggtcgg atccggcgga tcgctgcgga tgtacgccag ttcagagggc gccagcccgg
     7561 gatgcatttc cggccgacgg taaaggacca gccacgcgac gacgcagagc agcccgagcg
     7621 aaccggtgac gccgaacgcc catggccagc catatcgcac ggtgatccac gggacgatca
     7681 atggcgcgat gatcgcgccg acattagacc ccgcgttgaa caagccggtg gccagcgcgc
     7741 gctctttctt cggaaaccat tcgctcaccg ttttgatcgc ggcgggaaag ttgccgcctt
     7801 cggccagtcc aagtccaaat cgcgcagccg caaactgcgc cgccgttcgc gcgaacgcat
     7861 ggctcatcgc ggcgacgctc caaagcacga tcgcgatcgt atagccgatg cgcaggccga
     7921 cgcggtccat caatcgcccg gcgaacaggt atccgccggc atacgccacc tggaaccaga
     7981 agacaatgtt gctgtagtcg atctcgttcc agctgaattg cttttgaagc gtgggcttga
     8041 gaacgctgat cacttggcga tcgacataat taatcgtggt ggcgaagaac aacagcgcgc
     8101 agatccacca tcgcaacccg cccacgccgg cgccgccagg aggatcggat gcgattggat
     8161 tgagctggtc gctcgcggtc atgcaatcgt ctccgtcgcg ttggcgcatg cgccgaacgc
     8221 acctgggctt tccccctctc ccgcgaatac gtgggcgagg gaacccggac ctgcgcccac
     8281 cacgatggcc tacgaggccg gctgagttgt agcctttcgg cccgccgatg tcataatccc
     8341 cgccatgccg gccgccgagt ggaccaatga tgcagagctc ttccagctga tccgcgatgt
     8401 gctgtacacg cccgtcgtcg gcgacatcct cgacgacctc ggattcgtac accagttcct
     8461 gccgccgccg atccaaccgc tgcgcgagga gatgaagctg gccggccgcg cgatgccggt
     8521 gctgatgatc gacgtgttcg gcaaacagaa gcagccgttc ggcaagctga ccgaggcgct
     8581 ggaccaactg cagcccggcg aggtttatct cgccaccggc ggcgagatgc ggtgcgcgta
     8641 ctggggtgaa atcctcaccg ccaccgcgaa aaaacgcggc gctgtgggcg cgattatcaa
     8701 cggttttcac cgcgacacgc ccaaggtgct ggaacaagac tggccggtct tttcccgcgg
     8761 gcggttcgcc caagattcgg gcgtccgcac gcaggtggcg gactatcgct gcccgatcga
     8821 agtcggccac gtgcacgtcg aacctagcga cttgatattt ggcgatctc
//
LOCUS       DATIMH010000306         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3245184, whole genome shotgun sequence.
ACCESSION   DATIMH010000306 DATIMH010000000
VERSION     DATIMH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3245184"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VLI90_05225"
     CDS             <1..430
                     /locus_tag="VLI90_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSV13638.1"
                     /translation="GFAARGRGTLINVSSVLALAPERFNATYSGTKAFMLNLTLALRT
                     ELANTGIRVQAVLPGATRTEIWGKAGIDVAAMPADRVMDAGEMVDAALAGLDMGETVT
                     IPSLPDMTEWSRFDDARLAMGPNLSLAQAAPRYTKTGKAA"
     gene            481..1815
                     /locus_tag="VLI90_05230"
     CDS             481..1815
                     /locus_tag="VLI90_05230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV13639.1"
                     /translation="MLANLDLFIVNVALPNIAQDFGETSLGTLSWILNGYAIVYAALL
                     VFCGRFAERFPRNLSFLAGIALFTVASAACALSNSVESLVAFRLVQAAGAALLTPTSL
                     GLILATFPAERRGGAVRTWAAIGGFAAALGPLVGGLLVTASWRWIFLVNVPIGLLALA
                     VGWFKLPRVPGHDVPAPHLGAAVLITAGIATLTFGIVKLNDWGWNAPAVVLSFVSAAA
                     LLALFVAHCLRSDNPFIDPALFRIRDFTRATLTMAPFSTAFGGFLLSLVLWEEGVWHW
                     PAMMIGLAIAPGPFMVPVTSLLLSGRLIARFGAETVVATGLITFAIGVIAWAVLAGPH
                     PSLWFAVLCVIPSGIGVGLTLPTLMGFGTSALPASSFATGSGVINMVRQIGFAVGVAI
                     FVAVVGSPGSPEAHMAAFRLAWWLMTAITVIGLFPLFLLRRWRPAPAITKPA"
     gene            complement(1826..>2330)
                     /locus_tag="VLI90_05235"
     CDS             complement(1826..>2330)
                     /locus_tag="VLI90_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV13640.1"
                     /translation="AIVPFEDRHAPAVRALFTTVNRLLAPPGMRDAFEAYIARSLAEE
                     IDRITEYYADHDGGFWVALKADAVAGMFGLERCGPDAMELRRMYVDPSARRGGIARAM
                     LAFAEAECRRRGAARLELSTSEVQPAALALYRNSGFRLTREEAADTASNKTIGGGIRR
                     FYFEKLV"
BASE COUNT          308 a          739 c          810 g          473 t
ORIGIN      
        1 cggtttcgcg gcgcgtggac gagggacgct gatcaatgtg tcttcggtgc tcgctctggc
       61 gcccgagcgg ttcaacgcca cctacagtgg tacaaaggcc ttcatgctga acctcaccct
      121 ggctctgcgc accgagttgg cgaacaccgg catccgcgtc caggcggtgc tgcccggcgc
      181 gaccagaacc gaaatctggg gcaaggcggg gatcgacgtc gcggcgatgc cggcggacag
      241 ggtcatggac gccggagaaa tggtggacgc ggctctcgcg ggcctggaca tgggcgagac
      301 ggtgacgatc ccgtccctgc cggacatgac cgagtggtcg cggttcgacg acgcgcgtct
      361 ggcgatgggg ccgaatttat cgctggcgca ggcggccccg cgatacacga agacgggaaa
      421 agcggcgtga ccgcgccaac gaggcctggg caggtcctgg cgatcgtttg cgccggcatc
      481 gtgctggcca atctcgatct gttcatcgtc aacgtcgcgc tgccgaatat cgcccaggat
      541 tttggtgaga cgagcttggg gacgctgtcg tggattttga acggctatgc gatcgtttac
      601 gcggccttgc tggtgttttg cggcaggttc gccgagcgct tcccgcgaaa tctcagtttt
      661 ctggcgggca tcgccctgtt caccgtcgcg tcggccgcct gcgcgctctc gaacagcgtt
      721 gaaagcctgg tcgcgttccg tctggtgcag gcggccggcg cggcgttgct caccccgacg
      781 tctctgggct tgatcctggc cacgttcccg gcggaacgcc gcggcggcgc ggtccgcaca
      841 tgggccgcta tcggtggttt cgccgcggca ctggggccct tagtgggcgg tttgctggtc
      901 acggccagtt ggcggtggat attcctggtg aacgtgccga tcggtctgct cgcattggcc
      961 gtgggctggt tcaaactccc cagggtgccc ggtcacgacg ttcccgcacc gcatttgggg
     1021 gcggccgttc tgatcaccgc cggcatcgcg acgctgacat tcggaatcgt gaagctgaac
     1081 gactggggct ggaacgcgcc ggcggtcgtg ctgagcttcg tttcggcggc ggcgctgttg
     1141 gctttgttcg tcgcgcactg cctgcgatcc gacaatccgt tcatcgatcc cgctctgttt
     1201 cggatccggg atttcacgcg cgcgactttg acgatggccc cgttctcaac cgcgttcggc
     1261 ggtttcttgc tctcgctcgt gttgtgggag gaaggcgtgt ggcactggcc cgccatgatg
     1321 atcggtctgg cgatcgcgcc ggggcccttc atggttcccg tcacgtcgtt gttgctttcc
     1381 ggacgattga tcgcccgttt tggtgccgag acggtcgtgg cgacggggct gatcaccttc
     1441 gccatcggcg tgatcgcatg ggccgtgctc gccgggccgc acccaagttt gtggttcgcc
     1501 gtgctttgcg tgattccgtc gggcatcggc gtcgggctca cgctgccgac gctgatggga
     1561 tttggcactt cggcgctgcc ggcgtcgtcg ttcgcgaccg gatccggcgt gatcaacatg
     1621 gtgcgtcaga tcgggttcgc ggtcggtgtc gccattttcg tcgctgtcgt cggatcgccc
     1681 ggttcaccgg aggctcacat ggccgcgttc cgtctcgcct ggtggctgat gacggcgatc
     1741 accgtgatcg gcctcttccc gctgttcctg ctgcggcggt ggaggcccgc acccgccatc
     1801 acgaagccgg cgtgaggccg ggtcgtcaga ccagcttctc gaagtagaac cggcgaatgc
     1861 cgccgccgat ggtcttgttg ctggcggtgt ccgccgcttc ctctcgcgtc agccggaatc
     1921 cgctgttccg gtaaagcgcc agtgccgccg gctgcacctc cgacgtgctg agttccagtc
     1981 gcgcggcgcc gcgccgccgg cattcggcct cggcgaaggc gagcatggcg cgggcgatgc
     2041 cgccgcggcg cgccgacggg tcgacataca tccggcgcag ttccatcgcg tctggcccgc
     2101 atcgctccag cccgaacatg ccggcgacgg cgtccgcctt cagcgcgacc cagaagccgc
     2161 cgtcatggtc ggcgtaatat tcggtgatcc gatcgatctc ctcggccaat gagcgggcga
     2221 tataggcttc gaacgcatcg cgcatgcccg ggggtgcgag caggcggttg accgtggtga
     2281 acagggcgcg gacggcgggt gcgtggcgat cctcgaacgg gacgatcgct
//
LOCUS       DATIMH010000307         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_648751, whole genome shotgun sequence.
ACCESSION   DATIMH010000307 DATIMH010000000
VERSION     DATIMH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648751"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..2750)
                     /locus_tag="VLI90_05240"
     CDS             complement(192..2750)
                     /locus_tag="VLI90_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV13641.1"
                     /translation="MAFRPVLLLPLVIIIAIVAAARGQSLDDAQRLLRTGEYAKCDQI
                     CDAAIAGNDWRESWWLLKLRAELTTGQYGEALKTYESALARQPASVQIRLLGYQVLRA
                     NDRPADAISAINEIAQMIRAEPWRYSSARDRVAVGRAMLIAGSDARQILELLYDRAKK
                     DEPEAIEPYLASGDLALDKNDFAVAAESFEAAAKHATNDPDVYFGLARAYENDSQRAN
                     AALTKALELNPRHVPSLLYQADNAIDQEAYDAASEILDRVLSINLHESHAWAYRAVLA
                     HLLGDRKKESDARDQALSTWHTNPEVDHLIGKKISQKYRFVEGQAYQRRALEFAPDDQ
                     PARIQLCQDLLRLGQEDEGWQLAADVFKADPYNVLAFNLITLHDTMAKYSVLTSGGFD
                     VRMEPHEAAVYGQRVQALLARARQTLCEKYDVQLPETIQVEIFPQLKDFAIRTFGLPG
                     GAGYLGVCFGQVITANSPASRPGHPANWEAILWHEFCHVVTLNRTRNKMPRWLSEGIS
                     VYEETQANPAWGQTMNPQYRELILEKPEAVTPVSKLSGAFLKPPGAMELQFAYYESSM
                     VVRYIVDHFGLSAIKQILSDLADDAPINDALARHTEPIEKLDRDFADWLQAQAKALAP
                     DVDWERPDVALDAGSAALLAWVDQHPKSFFGQLAIGQALVAEQKWSDAKTHLERAVAL
                     YPTYGEVGGPYVLLAAVYRGFNDVPAERAMLEKHASLNTDAVEPRLRLLEILEQQKDW
                     PAVRKMAEQVLAVNPLIPAPHRYLADAAEAQGDRAVAIEANRTLLTMDPPDLADRHYH
                     LAKLLRANGQSADARREVVRALEEAPRFREAHRLLLEIVESSPTTASTTAPARP"
BASE COUNT          484 a          959 c          809 g          540 t
ORIGIN      
        1 gccgccgcca ccgtagcgac gttgctgttg ggcgatgacg aggctggcaa cgcatagcaa
       61 ccccagtgcg agggcgatga tggtggtggg ccggcgtctc ataggcgttc gagtgtacgt
      121 ccgtcatagc cgggggcgga aaccccggtc tgttttccgt ccgaatggac cggggcttcc
      181 gcccccggct atcaaggtcg cgccggcgct gtagtcgacg ccgtcgttgg tgaagattcg
      241 acgatctcca gcaacagccg atgcgcttcg cgaaaccgcg gggcttcttc cagcgcgcga
      301 acgacttcac gccgcgcatc agccgattgt ccgttcgcgc gcagcagctt cgccagatga
      361 tagtgccgat ccgccaggtc cggcgggtcc atcgtcagca gcgtgcgatt cgcttcgatg
      421 gcgacggctc gatcaccctg cgcctcggcc gcgtcggcca aatatcgatg cggcgccgga
      481 atcagcggat tcaccgccag cacctgctcg gccatctttc gcacggccgg ccaatccttt
      541 tgctgctcga ggatctccag caaccgcaac cgcggctcca cggcgtcggt gttcagggac
      601 gcgtgcttct ccaacatcgc tcgctcggcc ggcacgtcat tgaacccgcg atacaccgcc
      661 gccagcagca cgtacggccc accgacttcg ccgtaggtag gatacagcgc caccgcccgc
      721 tccaggtgtg ttttcgcgtc cgaccatttc tgctccgcga ccagcgcctg cccgattgcc
      781 agctggccga agaaactctt tggatgctga tctacccacg ccagcagcgc agcgctgccg
      841 gcgtccagcg cgacatccgg tcgctcccag tccacatctg gcgccagcgc cttcgcctgc
      901 gcttgcagcc aatcggcaaa atcgcgatcg agcttttcga tcggctccgt atgccgcgcg
      961 agggcgtcgt tgatcggcgc atcgtcggcc aagtcgctca agatctgctt gatcgccgac
     1021 aggccgaagt gatcgacgat ataacgcacg accatcgacg actcgtaata cgcgaactgc
     1081 aattccatcg cccccggcgg tttcaagaac gcgccgctca gcttgctcac cggcgtgacc
     1141 gcctccggct tttccaggat cagctcgcga tactgcgggt tcatcgtctg tccccacgcg
     1201 gggttcgcct gcgtttcttc atagacgctg atgccttcgc tcagccagcg cggcatcttg
     1261 ttgcgcgtcc tgttcaatgt gacgacgtga cagaattcat gccacaggat cgcttcccag
     1321 ttcgccggat ggccggggcg agacgcgggg ctgttggcgg tgatgacctg gccgaaacaa
     1381 acgccgaggt aaccagcgcc acccggcaaa ccaaacgtgc ggattgcgaa gtctttcagt
     1441 tgcggaaaga tctcgacctg gatcgtctcc ggcagctgca catcgtattt ctcgcacaat
     1501 gtctgccgcg cccgggccag cagcgcctgc acacgttggc cataaaccgc cgcctcgtgc
     1561 ggctccatcc gcacgtcaaa cccgccgctc gtcagcacgc tgtacttcgc catcgtgtcg
     1621 tgcagcgtga tgaggttgaa cgccagcacg ttgtatggat cggccttgaa tacgtcggcc
     1681 gccagctgcc agccttcgtc ctcctgcccc agccgcagca ggtcctggca cagctggatg
     1741 cgcgccggct gatcgtctgg cgcgaattcc aacgcccgcc gttgatacgc ctgtccctcg
     1801 acaaaccggt atttttgcga gatttttttg ccgatcaggt gatcgacctc ggggttcgtg
     1861 tgccaggtcg agagcgcctg atcgcgcgca tcgctttcct tcttgcgatc cccaagcaga
     1921 tgagccaaca ccgcgcgata cgcccacgcg tggctctcgt gcagattgat ggacagcacg
     1981 cgatccaaaa tctcgctcgc ggcgtcgtat gcttcctggt cgatcgcgtt gtccgcctga
     2041 tagagcaaac ttggcacatg ccgcgggttc agctccagcg ccttggtcag cgctgcgttg
     2101 gcgcgctgcg aatcgttctc gtacgcgcgg gccaagccaa aataaacatc aggatcgttc
     2161 gtcgcgtgct tcgccgccgc ttcgaacgac tcggctgcga cggcaaaatc gttcttgtcc
     2221 agcgccaggt ccccgctggc cagatacggc tcgatcgcct cgggctcgtc cttcttcgcg
     2281 cgatcgtaca acagctccag gatctgccgg gcatcgctgc cggcgatcaa catggcccga
     2341 ccaaccgcca cgcgatcgcg cgcgctgctg taccgccacg gttccgcgcg gatcatctgg
     2401 gcgatttcat tgatggcgga aatggcatcg gcggggcggt cgttcgcccg cagcacctga
     2461 taacccagca accggatctg cacgctcgcc ggctggcgcg ccagcgccga ttcgtacgtc
     2521 ttcaatgcct cgccatattg ccccgtcgtc agctccgcgc gaagcttcag cagccaccac
     2581 gactcccgcc aatcattgcc cgcaatggcg gcgtcgcata tctggtcgca cttagcatat
     2641 tcgccggtgc gcaacagccg ctgcgcatcg tccagcgatt gtccgcgcgc cgcggcaacg
     2701 atcgcaatga tgatgaccag cggtaacagc aggaccggcc gaaacgccat atgtctatcc
     2761 taccgggaaa tcgacgccgc cgttgagcaa ga
//
LOCUS       DATIMH010000308         1981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4399330, whole genome shotgun sequence.
ACCESSION   DATIMH010000308 DATIMH010000000
VERSION     DATIMH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4399330"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VLI90_05245"
     CDS             <1..1092
                     /locus_tag="VLI90_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HSV13642.1"
                     /translation="LSDITESNGSSSMATVCGGSLSMMDAGVPLKRPVSGIAMGLILE
                     GKDFAVISDILGDEDHLGDMDFKVAGTSEGITSLQMDIKIAGITEEIMKAALAQAKEG
                     RAHILGEMAKALDHTREELSAHAPRIETMQIPKDKIREVIGTGGKVIREIVATTGAKV
                     DIDDEGLIKISSSDLSQIEAARQWIHGIVAEPEPGTIYTGKVASIVDFGAFVTFMPGK
                     DGLVHVSEIKNERVENVRDVLSEGQEVKVKLVEVDQRGKVRLSMRLVDQETGEELPDT
                     RPAREPREGGDRGPRGEGRGDRGDRGRGGRDRGPRRDGDRGPRREGGDRGDRGPHGEG
                     RGDRGPRREGGDDNGPAPEFSPAFLTGED"
     gene            complement(1149..>1981)
                     /locus_tag="VLI90_05250"
     CDS             complement(1149..>1981)
                     /locus_tag="VLI90_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSV13643.1"
                     /translation="RAPRQIISLWNSLKQPDLKESDMWGMSPIDQMVCRIQFRQSDYR
                     GEFTPPRADKHTVQYPGYNGGSDWGGVALDPMRGVLVVNYNDMPNYLRLVPRAEANRL
                     GIVPRFASKQGAPTNAHSIDAQWGVPYAINVDAGWRLKFTKLMCKRPPYGGIRAIDVA
                     TGKTLWDRPFGTARRNGPFNIPTMLPFSIGTPNNGGAVTTASGLVFIAAATDDLIRAI
                     DLKTGKTVWSAPLPAGGQANPIIYQQDGREYLVIVAGGHHFMETPMGDSVVAYALPKA
                     "
BASE COUNT          356 a          637 c          653 g          335 t
ORIGIN      
        1 ctctccgaca ttacggagag caacggctcg tcttcgatgg cgaccgtctg cggcggctcg
       61 ctgtcgatga tggacgctgg cgtcccgctg aagcggccgg tgtccggcat cgccatgggc
      121 ctgatcctcg aaggcaagga cttcgcggtg atcagcgaca tcctcggcga cgaggaccat
      181 cttggcgata tggacttcaa ggttgccgga acgtccgagg gcatcaccag ccttcagatg
      241 gacatcaaga tcgccggcat caccgaggag atcatgaagg ccgccctcgc ccaggcgaag
      301 gaaggccgcg ctcatatcct cggcgagatg gccaaggccc tcgatcacac gcgcgaagag
      361 ctgtcggctc acgcgccgcg catcgagacc atgcagatcc cgaaggacaa gatccgcgaa
      421 gtcatcggca ccggcggcaa ggtcatccgc gagatcgttg cgacgaccgg cgccaaggtc
      481 gacatcgacg acgaaggcct gatcaagatc agctcgtccg acctgtcgca gatcgaagct
      541 gcccgccagt ggatccacgg catcgttgcc gagcccgagc cgggcacgat ctacaccggc
      601 aaggttgcga gcatcgtcga tttcggcgcg ttcgtgacct tcatgccggg caaggatggt
      661 ctggttcacg tctcggaaat caagaacgag cgtgtcgaga acgtccgcga tgtcctgagc
      721 gaaggccagg aggtcaaggt caagctggtc gaggtcgatc agcgcggcaa ggttcgcctg
      781 tcgatgcgcc tcgtcgacca ggaaactggc gaagagctgc ctgatacccg cccggcgcgc
      841 gagccgcgtg agggtggtga tcgcggaccg cgcggcgaag gccgtggcga ccgcggcgat
      901 cgtggccgtg gcggccgcga ccgtggcccg cgccgcgatg gcgatcgcgg accgcgccgt
      961 gaaggcggcg accgcggcga tcgtggcccc cacggtgaag gccgtggtga tcgtggaccg
     1021 cgtcgcgaag gtggcgacga caacggcccg gcgccggagt tctcgcccgc cttcctgacc
     1081 ggcgaagact aagtcttcag catcacagag aaattggggc cgtcggcatc acgccggcgg
     1141 cccctttttc atgccttcgg aagcgcgtag gcgacgacac tgtcgcccat cggcgtctcc
     1201 atgaagtggt ggccgccggc cacgatcacc agatattcgc ggccatcctg ctggtagatg
     1261 atcgggttcg cctgtccgcc cgcaggaagc ggcgccgacc agaccgtctt tccagtctta
     1321 agatcgatgg cgcggatcag gtcgtcggtc gccgcggcaa tgaacaccag cccgctcgcg
     1381 gtcgtcaccg cgccgccatt gtttggcgtg ccgatgctga acggcagcat ggtcgggatg
     1441 ttgaacggcc cattccgccg cgcagtgccg aacggccggt cccacagcgt cttgcccgtg
     1501 gcgacgtcga tcgcccggat tccgccgtag ggcggccgct tgcacatcag tttggtgaat
     1561 ttcagccgcc agccggcatc gacattgatc gcataaggca cgccccattg agcatcgatc
     1621 gaatgggcgt tggtcggcgc accctgcttg ctggcgaaac gcggcacgat gcccagcctg
     1681 ttggcttcgg ccctcggcac cagccgcagg tagttcggca tgtcgttgta gttgacgacg
     1741 agcacgccgc gcatcgggtc cagtgcaacg ccgccccagt cactgccgcc attgtaaccc
     1801 ggatactgga cggtgtgctt gtcagcccgc ggcggcgtga actcgccgcg ataatcggac
     1861 tggcggaact ggatgcggca gaccatctgg tcgatcggcg acatgcccca catgtccgat
     1921 tccttgagat ccggctgctt caggctgttc cagagcgaga ttatctgacg cggcgcacgt
     1981 t
//
LOCUS       DATIMH010000309         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_70504, whole genome shotgun sequence.
ACCESSION   DATIMH010000309 DATIMH010000000
VERSION     DATIMH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70504"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VLI90_05255"
     CDS             <1..541
                     /locus_tag="VLI90_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13644.1"
                     /translation="IAAFVLLYDARAKRHPIAGPVCMGTCRGLNLLLGVSIDGGALGA
                     HWPLGLLPLVYIAAVTTVSRGEVHGGRKPVATFALISLALVLTGLIAVAVNSTMGGRG
                     AALVLVAVLGYRVLPPYWQVRKNPDPVTIRTAVRAGVLSLVLLDSAIGAAYAGPLYAA
                     IILAAGVAAGALARVFSVT"
     gene            544..1773
                     /locus_tag="VLI90_05260"
     CDS             544..1773
                     /locus_tag="VLI90_05260"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF004852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HSV13645.1"
                     /translation="MDVIQQEFLVPVRYPVHFTTGVFAASNRVLRDVIADGRSATASA
                     PNGEDRLPARVVVVLDRGVERAHPTLVDTIRAYADQHRDALTLATPVLVVPGGEQIKE
                     DRRHADRIVEAINAASLCRHSYVIAVGGGAVLDAAGFAAATAHRGVRLVRVPTTVLSQ
                     DDSAVGVKNGINAFGKKNYLGTFSPPFAVINDAGFLATLSDRDWRGGTTEAVKAALIR
                     DPQFFELLEQSATSLADRDLAAMAQVIRHSAILHLRHIATSGDPFEHGSSRPLDFGHW
                     AAHKLEQVTQHRLGHGEAVGIGIAIDSTYSYLAGYLPEASWRRIVQLLVALGVPVYDP
                     ALDERLDTPDHPDCVLKGLDDFQEHLGGRLTVMLLKDIGQPFDVHEVRPDLVRTSIAM
                     MKAREAERAASSAKKAS"
     gene            1770..2084
                     /locus_tag="VLI90_05265"
     CDS             1770..2084
                     /locus_tag="VLI90_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13646.1"
                     /translation="MKTHPAGCPLSGTQIVDEYFIENRTRLLEIAAFLDRLDRVDREC
                     AMRDFRMQVFRDAVDALGTPGDRLNRIQLLLSDPTIEPLDRLDRKSALGAYDRQAKES
                     RS"
     gene            2081..2917
                     /locus_tag="VLI90_05270"
     CDS             2081..2917
                     /locus_tag="VLI90_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSV13647.1"
                     /translation="MRYIDHHAHMVSRTTDDYQQLALTGCVAVTEPAFWAGWDRSCGD
                     SVEDYFRQLTDFEPRRAAQYGIQHYTWMAINPKEADDRVLSRDVLARIPKFLDHPTVL
                     GIGEIGMNRITRNEIATYTEQVELAIAHHQLILIHTPHLEDKLKGTRISVDVLRRYRE
                     IDPGRVLIDHAEEHTLDLILEHGFWAGLTLYPQTKISAQRAVDVIEKHGPDRICVAGA
                     CDWGPSDPIAVPRLIMEMRRRRHPEALIERVVFENPARFLSQSPKFKVTATGAAQPAR
                     VG"
     gene            2930..>3132
                     /locus_tag="VLI90_05275"
     CDS             2930..>3132
                     /locus_tag="VLI90_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13648.1"
                     /translation="MQLDAPHPFHLTYCTNIHAADGWTAVFENVRQFAPALKDRLSPT
                     APFGIGLRLSARDARELLQDERL"
BASE COUNT          529 a         1023 c         1007 g          573 t
ORIGIN      
        1 gatcgctgcg ttcgtgctcc tgtatgacgc tcgcgcgaag cggcatccca tcgcgggtcc
       61 ggtctgcatg ggaacgtgtc gcggactgaa cctgttgctc ggcgtgagca tcgatggcgg
      121 ggcgctcggt gcgcattggc cactgggact gcttccgctt gtgtacatcg cggcggtcac
      181 caccgtcagc cgcggcgagg tccatggcgg acgcaagcca gttgcgacgt ttgctttgat
      241 atcgttagcg ctggtgctga cgggcttgat cgcggtggcg gtcaattcga cgatgggtgg
      301 ccgcggcgcg gcgctcgtgc tcgtcgcggt ccttgggtat cgagtgctgc cgccgtactg
      361 gcaggtgagg aagaatccgg acccggtgac gatccggacc gcagtcaggg caggcgtgct
      421 gtcgctggtc ctgctcgatt cggccatcgg cgctgcgtat gctggtccgt tgtacgccgc
      481 gatcattctt gcggcaggtg tggcggccgg cgcgctcgcc agggtgttct cggtaacgtg
      541 atgatggatg tcatccagca ggagtttctc gtcccggttc gctacccggt gcatttcacc
      601 accggtgtgt tcgcagcgtc gaatcgggtg ctccgcgacg tcatcgccga cgggcgatcc
      661 gcgacggcgt ccgcgcccaa cggagaagac cgcctgcccg ccagggtcgt ggtcgtcctc
      721 gatcgcggcg tcgagcgcgc tcatcccacg ctggtcgaca ccatccgcgc ctacgctgac
      781 cagcatcgtg acgccttgac cctcgcgacg cccgtgctgg tcgtcccggg aggcgagcag
      841 atcaaagaag atcgccgaca cgccgatcgc atcgtcgagg cgattaacgc ggcgtcactg
      901 tgccgtcact cgtatgtgat cgccgtcggc ggcggcgcgg tactcgacgc ggcggggttc
      961 gccgcagcga ccgcgcatcg cggggtccgg ctcgtccggg ttccaacgac cgtgctgtcg
     1021 caggatgatt ctgccgtcgg cgtcaagaac ggaatcaatg cgttcgggaa gaagaattac
     1081 cttggcacgt tctcgccgcc cttcgcggtc atcaatgatg ccggctttct tgcaacgctg
     1141 tcggatcgcg actggcgcgg cgggaccacc gaagcggtga aggccgcgct cattcgcgac
     1201 ccgcagtttt tcgagcttct cgaacaaagc gccacctcgc tcgccgatcg cgacctggcg
     1261 gccatggcgc aggtgatccg gcactcggcc atcctgcacc tccgtcacat tgcgaccagt
     1321 ggagatccgt tcgagcacgg gtcctcgcga ccgttggact tcggtcactg ggcggcgcac
     1381 aaactggaac aggtcacaca gcatcgcctt gggcacggcg aggcggtcgg tatcggcatc
     1441 gccatcgaca gcacctactc gtatctggcg ggatatcttc ccgaagcgtc atggcggcga
     1501 atcgtccagc tgctggtcgc cctcggcgtg ccggtgtacg atcctgcgct ggacgagcgc
     1561 ctcgatacgc ccgatcatcc tgattgcgtg ctgaaaggcc tcgatgattt ccaggaacac
     1621 ctgggagggc ggctcacggt catgctgctc aaagacatcg gtcagccgtt cgacgtgcac
     1681 gaggtgcgtc cggatctcgt gcgcaccagc atcgcgatga tgaaagcgcg cgaggccgag
     1741 cgcgcggcat cctctgcaaa gaaggcctca tgaagacgca ccctgccggc tgcccgctgt
     1801 ccggcacgca aatcgtcgac gagtatttca tcgagaaccg cacccggctg ctcgaaatcg
     1861 ccgccttcct cgatcggctc gaccgcgtcg atcgcgagtg cgcgatgcgc gatttccgca
     1921 tgcaggtgtt ccgcgatgcg gtcgatgcgc tgggcacgcc gggcgatcgg ttgaatcgca
     1981 ttcagctcct cctcagcgat ccgacgatcg agccgctcga tcggctggat cgcaagagcg
     2041 ctctcggcgc ctacgatcgc caggccaagg agtcacggtc gtgagatata tcgaccacca
     2101 cgcgcacatg gtctcgcgca ccaccgacga ctatcaacag ctggcgctga ccggctgcgt
     2161 cgccgttacc gaaccggcct tctgggccgg atgggatcgc agctgcggcg acagcgtgga
     2221 agactacttc cgacagctga cggacttcga gccgcgccgc gccgcgcagt acgggatcca
     2281 gcattacacc tggatggcga tcaacccgaa agaggccgac gatcgggtgt tatcgcggga
     2341 tgtgctggcg cgaattccca aatttctcga tcatccgacg gtgctcggca tcggcgagat
     2401 cgggatgaat cgaatcaccc gcaacgagat cgcgacgtac accgaacagg tcgaactggc
     2461 gatcgcacac catcagctga tcctcattca cacgccgcac ctcgaggaca agctgaaggg
     2521 gacgcgaatc tcggtcgatg tgctgcggcg ctaccgcgag atcgatcccg gtcgcgtcct
     2581 catcgatcac gccgaggagc acaccctcga tctcatcctc gagcatgggt tctgggccgg
     2641 actgacgctt tatccgcaga cgaagatctc ggcgcagcgc gcggtcgacg tgatcgagaa
     2701 acacgggccc gatcgcatct gcgtcgcggg cgcctgcgac tggggcccaa gcgatccgat
     2761 cgccgttccc agattgatca tggagatgcg tcggcggcgc catcctgaag cgctcatcga
     2821 gcgggtcgtg ttcgagaacc cggcgcgttt tctcagccag tcaccgaagt tcaaggtgac
     2881 cgccaccggc gccgcgcagc cggcgcgggt cggctgaggc gacgccgctt tgcaactcga
     2941 cgcgccgcat ccgttccacc tgacctactg tacgaacatc catgcggctg acggatggac
     3001 cgccgtcttc gagaacgtcc gccagtttgc tcccgcgctg aaggatcgcc tctcaccgac
     3061 cgccccgttt ggaatagggc tgcgcctgtc ggcacgcgac gcgcgtgagc tgctccagga
     3121 cgaacgcctg ca
//
LOCUS       DATIMH010000310         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2378274, whole genome shotgun sequence.
ACCESSION   DATIMH010000310 DATIMH010000000
VERSION     DATIMH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378274"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /gene="pgsA"
                     /locus_tag="VLI90_05280"
     CDS             complement(<1..457)
                     /gene="pgsA"
                     /locus_tag="VLI90_05280"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HSV13649.1"
                     /translation="MNLPNAITIGRIFLVPLLVVVLLTKFEGRLILGIRKELVGAAIF
                     GIASLTDWLDGYLARRRKQVTPLGQFMDPLADKLLITAALVSLVQMDLAQSWMVVVIL
                     GREIAVTVLRSLAQSRGVSIPASPLGKFKMVSEVTAILLLILGDHIPMFA"
     gene            complement(454..609)
                     /locus_tag="VLI90_05285"
     CDS             complement(454..609)
                     /locus_tag="VLI90_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13650.1"
                     /translation="MLNFEGVVRGEEVNNQQSTIHPELVDDWGVKGTRARNVTTQPSA
                     FNIQQSL"
     gene            648..1316
                     /locus_tag="VLI90_05290"
     CDS             648..1316
                     /locus_tag="VLI90_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSV13651.1"
                     /translation="MKAILLAGGKGTRLRPLTVHTPKPIVPILDRPFLYYQIDLLKQV
                     PEIDEVILSLNYQPRRIEEIFGEGADLGVRLRYVVEPMPLGTGGAIRYAGDSLTDSVV
                     VFNGDVLTEVDLGAVLRLHRERRAKATIVLTPVDNPRAYGLVETDASGNILRFLEKPG
                     EDEITCNTINAGIYVLEPETFDRIPKDTAWSIERSFFPSLIERGETFVAYVYDGYWID
                     IGTP"
     gene            1344..1706
                     /locus_tag="VLI90_05295"
     CDS             1344..1706
                     /locus_tag="VLI90_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HSV13652.1"
                     /translation="MDGRYKAPPFAGAPAATCWVSPDAKVEQGVELHGPCFIDDGAVV
                     KAGAKILPYAVIGRQTHVSEGAVIDGSIIWPNGWIGPEATVRSSILGRNCHIGRNVTI
                     ETPVQLGDKTAITDYSRL"
     gene            1703..3073
                     /locus_tag="VLI90_05300"
     CDS             1703..3073
                     /locus_tag="VLI90_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HSV13653.1"
                     /translation="MTTNSSTINTSIFKAYDVRGLYPGEVNEDAARQIGRAFVAYLKA
                     KRIAVSRDMRLSSPSVAAAFIDGARQQGADVVDYGMLATDMMYFAVARDGHDGGAQIT
                     ASHNPKQYNGIKLVRREAFPLSGEEGLGDIRDMIVKQTIPAAAGRSGSLTQMDVLDDY
                     VKHVLSFIDTRIIKPFNVVLDAGSGMGGLVAPKLFDRLPCRTTKLCFDIDGTFPNHEA
                     NPLIEENRRDIVERVVRDHADIGIAWDGDADRCFFIDGTGEFIAGDFVTALLAEAFLL
                     KNPGAKVVYDVRASYAVKDMVAKYGGTALMNRVGHAFFKKRMREEGAIFGGEVTGHYY
                     FRDNFYADNGFIPAMLMLELMSHKGQTLHELVAPLRQKYFISGEINTKMPDMRVVQEK
                     VDALAARYADAKVYSMDGTSVEYPDWHFNVRASNTEPLIRLNLEATTQQMMEQKREEV
                     LAFIRA"
BASE COUNT          649 a          942 c          944 g          578 t
ORIGIN      
        1 cggcaaacat cgggatgtgg tcgcccagaa tcaacagcag gatcgccgtc acttccgaca
       61 ccatcttgaa cttgcccagc ggtgatgcgg ggatcgacac gccgcgtgat tgcgccaggc
      121 ttcgcagcac ggtcacggca atctcgcgcc cgagaatgac gaccaccatc cacgattggg
      181 cgagatccat ctgcacgagc gagacgagtg cggcggtgat cagcagcttg tcggccagcg
      241 gatccatgaa ctggccaagc ggcgtcacct gcttgcgtct tcgcgccaga tagccatcca
      301 accagtcggt caacgacgcg atgccgaaga tggccgcgcc caccagttct ttgcgaatac
      361 cgagaatgag gcgcccctcg aatttcgtca gcaggaccac gacgagaaga ggaaccagga
      421 agatccggcc gatcgtgatc gcgttcggca gattcaaagg gactgctgaa tgttgaaggc
      481 tgaaggttga gtggtcacgt ttcgcgcacg cgttcctttt acgccccaat catcgaccaa
      541 ttccgggtgg attgtcgatt gctgattgtt gacctcttcg ccgcgaacga cgccttcgaa
      601 attcagcacc cggcattcgg ccgattcgca ttattatcgg aagttgcatg aaggcgattc
      661 ttctcgcagg cggtaagggc acgcggctga ggcctctgac ggttcacacc cccaagccga
      721 ttgtgcccat cctcgaccgg ccgtttctct attaccagat agacctgctc aagcaggtgc
      781 cggaaatcga cgaggtcatt ctcagcctca attaccagcc gcgccgcatc gaggaaatct
      841 tcggtgaagg agcggatctc ggtgtccgct tgcgctacgt cgtcgagccg atgccgctcg
      901 gaacgggcgg cgcgatcagg tatgccggcg actcgctgac cgactcggtc gtggtgttca
      961 acggcgatgt gctgacggaa gtcgatcttg gcgcggtcct gcggctgcat cgcgagcggc
     1021 gggcgaaagc cacgatcgtg ctcacgccgg tcgacaaccc gcgcgcctac gggctggttg
     1081 aaaccgatgc gtcgggcaac atcctgagat ttctggagaa gccgggcgag gacgagatca
     1141 cctgcaacac gatcaatgcc ggcatctacg tgctcgagcc ggagaccttc gatcgcattc
     1201 ccaaagatac ggcgtggtcg atcgaacgca gcttcttccc gtcgctgatc gagcgcggtg
     1261 aaacgttcgt cgcctacgtc tacgacggct actggatcga catcggcacg ccgtgaaaat
     1321 acctgcaaga ccatcgcgac atcatggacg ggcgatacaa ggcccccccg ttcgccggcg
     1381 cacccgcggc aacgtgctgg gtctcccccg atgccaaggt cgaacaaggg gtcgagcttc
     1441 acggtccgtg ttttatcgac gatggtgcag tggtgaaggc gggagcaaag attctgccgt
     1501 atgccgtgat cggacgacag acacatgtct ccgaaggcgc cgtgatcgat ggctcaatca
     1561 tctggccgaa cggctggatt ggtcccgagg cgacggtgcg ttcatcgatc ctcggccgca
     1621 actgtcacat cgggcgcaac gtcaccattg aaaccccggt gcagctcggg gacaagaccg
     1681 ctatcaccga ctacagccgc ttatgaccac caactcctcg actatcaaca cgtccatctt
     1741 caaggcctac gacgtccgcg gtctttatcc gggtgaagtc aacgaggacg ccgcacggca
     1801 gatcgggcgc gccttcgtcg cctatctgaa agccaagcgc attgctgtct cgcgtgacat
     1861 gcgcctcagc tctccgtcgg tcgcggctgc gtttatcgac ggcgcgcgcc agcagggggc
     1921 agacgtcgtc gattacggga tgctcgccac cgacatgatg tactttgccg tggcgcgtga
     1981 cggccacgac ggtggcgcgc agatcaccgc gtcgcacaat ccgaagcagt acaacggcat
     2041 caagctggtg cgccgtgaag ccttcccgct gagcggcgaa gagggactcg gcgacattcg
     2101 cgacatgatc gtgaagcaaa cgattccggc tgccgctggg cggtcaggat cgctcacgca
     2161 gatggatgtg ctcgacgact acgtcaagca cgtactgtcg ttcatcgaca cacgcatcat
     2221 caaaccgttc aacgtggtcc tcgacgcggg aagcggcatg ggcgggctgg tggcaccgaa
     2281 gctgttcgac cgactcccct gccggaccac gaagctctgt ttcgacatcg acggcacgtt
     2341 ccccaatcat gaagcgaacc cgctgatcga agaaaaccgc cgcgacatcg tcgaacgcgt
     2401 cgtgcgagac cacgcggaca tcgggatcgc ctgggatggc gatgccgatc ggtgcttctt
     2461 catcgacgga accggcgagt tcatcgccgg cgatttcgtc acggcgctac tcgcggaagc
     2521 attcctgttg aagaaccccg gcgccaaggt ggtctacgac gttcgcgcca gctacgcggt
     2581 gaaggacatg gtcgccaaat acggcggcac ggcgttgatg aaccgcgtcg ggcatgcgtt
     2641 cttcaagaag cggatgcgcg aagaaggggc gatcttcggc ggcgaggtga ccggtcacta
     2701 ttacttccgc gacaacttct atgcggacaa cgggttcatt ccggcgatgc tgatgctcga
     2761 actgatgtcc cacaaagggc agacgttgca cgagctggtt gccccactgc gacagaagta
     2821 tttcatctcc ggcgagatca acaccaagat gccggatatg cgcgtcgtcc aggaaaaggt
     2881 cgatgcgctt gcggcgcgct atgcggacgc gaaggtctat tcgatggacg gcacgtcggt
     2941 cgagtacccg gattggcatt tcaacgttcg cgcgtcaaat accgagccgc tgatccggct
     3001 gaatctggaa gccacgacgc agcaaatgat ggaacagaag cgggaggagg ttctcgcgtt
     3061 catcagggct taaggctcaa ggctcacgag cttgaggcgt gagccctcag cct
//
LOCUS       DATIMH010000311         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3246156, whole genome shotgun sequence.
ACCESSION   DATIMH010000311 DATIMH010000000
VERSION     DATIMH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3246156"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2310)
                     /locus_tag="VLI90_05305"
     CDS             complement(<1..>2310)
                     /locus_tag="VLI90_05305"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HSV13654.1"
                     /translation="VTILLDYTFNLPAVPRVLLMLVAFAAMALTLWHWVIQPLLVKLS
                     LGDVAGRLEQAFPQFQDRLRSTVDIITGHIPGSEMMKQRIVTETTNLALHVDLTRGIV
                     VRPVWYSTAAGLGSILLAVILMLAIGSQYTETALTRLFLPFADRTWPKTVQMDLVGNV
                     PSRVPVGQRLDINMRLARGDKASRKATIFYQYGDDAGQHFGPVEQEYMTRGDDGVYHA
                     SLDSRVMSDAATTGAMKIWMTAGDDQLAVTPVKVVQRLALRGVTAVVTPPPYAKQPPV
                     TINLAQNPAVMTVGSQVQLTAAFNKPLDAAKAPVIEALTGKAAPALDWRRPDVQTISG
                     TLTASESLRFRVHATDVDGFQNTAVEEYELIVRPDQNPTVQIENPRRNEDRTPVAVVP
                     FQAVAEDDFGINKLTLVVDRLGDKKHWEIPLIQGASATNGVTWNKIDAAGDLQRFRAN
                     YSWDLAALNGANLKPGDVLEFFAVVQDNYELNGQTHAPVPSGKLRINIISQDEFSNKI
                     TDEMRTIGEQVAQVKQGQGRTQRETASLTKQSADKPAFDAGDKVAADRLESQQSNAAA
                     QTKQLSGKLDDIGRRLEENKSTNTELKDTARDVGDLLNKTAENPMKDAAGSINAAKQS
                     PAKNDRNQKMQAAQDNQQTAGAQLQKALDRMGTIGSLSRTIDQIKALLEEQQRISNET
                     QAAGKNNLGKRPEEMDPKDRQKLEQAAKDQAELGNRTAKALDDMQKMADKLGKSDPSA
                     SSAMKEAASTGQQQNVPQQQSKAAQAAQQ"
BASE COUNT          333 a          794 c          694 g          489 t
ORIGIN      
        1 tctgctgcgc cgcctgcgcc gccttcgact gctgctgcgg cacattctgc tgctggcccg
       61 tgctggcggc ctccttcatc gccgacgacg cggatggatc ggacttgccc aacttgtccg
      121 ccatcttctg catgtcatcc agcgccttgg cggtgcgatt gcccagctcc gcctggtcct
      181 tcgccgcctg ctccagcttc tggcggtcct tcggatccat ctcctccggc cgcttgccaa
      241 gattgttctt ccccgccgcc tgcgtttcgt tgctgatgcg ctgctgctct tcgagcagcg
      301 ctttgatctg atcgatcgtg cgcgacaggc tgccgatcgt gcccatgcgg tccagcgcct
      361 tctgcagttg cgcgcccgcc gtttgctgat tgtcctgcgc cgcctgcatc ttctggttgc
      421 ggtcattctt cgcgggcgac tgcttcgccg cgttgataga gcccgcggca tccttcattg
      481 ggttctccgc cgtcttgttc agcagatcac cgacatcgcg cgccgtgtcc ttcaactccg
      541 tgttagtcga cttgttctcc tccagccgcc ggccgatgtc gtccagcttc ccggacagtt
      601 gcttggtctg cgccgccgcg ttgctctgct ggctttccaa tcgatcggcc gcgactttgt
      661 cgccggcgtc gaacgccggc ttgtccgcgc tctgcttggt cagcgacgcc gtctcgcgct
      721 gcgtccgccc ctggccctgc ttcacctgcg caacctgctc gccgatcgtc cgcatctcat
      781 cggtgatctt gttggaaaat tcatcctggc tgatgatgtt gatccgcagc ttgccgctcg
      841 gcaccggcgc gtgcgtctgg ccgttcagtt catagttgtc ttgaacgacg gcgaagaact
      901 cgagcacatc gcccggcttc aaattcgcgc cgttcagcgc cgctagatcc caggaatagt
      961 ttgcgcgaaa ccgctgcagg tcccccgccg cgtcgatctt gttccaggtc acgccgttcg
     1021 tcgccgacgc gccttgaatc agcggaatct cccaatgctt tttgtcgccg agccgatcga
     1081 cgaccagcgt cagcttgttg atgccgaaat catcctcggc gacggcttgg aacggaacga
     1141 cagcgacagg cgtgcgatct tcgttgcgcc gcggattctc gatctgcacc gtcgggttct
     1201 ggtccggccg gacgatcagc tcgtactctt cgacggccgt gttctgaaac ccgtcgacat
     1261 cggtcgcgtg cacgcggaaa cgcagcgatt cgctcgccgt cagcgtgccc gagatcgtct
     1321 gcacatccgg ccgccgccaa tccaacgccg gggcggcttt gcccgtcagc gcttcgatga
     1381 ccggggcctt cgccgcgtcc agcggcttgt tgaacgccgc cgtcaactgc acctgcgaac
     1441 ccaccgtcat gacagcgggg ttctgagcca ggttgattgt caccggcggt tgcttggcgt
     1501 acggcggcgg tgtgacgacc gcggtgacgc cgcgaagggc gagccgctgc acgactttca
     1561 ccggcgtcac ggccagctgg tcgtcgccgg cggtcatcca gatcttcatc gcgcccgtcg
     1621 tggcggcgtc gctcatcacg cgcgagtcga gcgacgcgtg atatacgcca tcgtccccgc
     1681 gcgtcatgta ttcctgctcg accggcccga agtgctgccc ggcgtcgtcg ccgtattgat
     1741 agaagatggt tgccttgcgc gacgccttgt cgccgcgcgc caggcgcatg ttgatgtcca
     1801 gccgctgccc caccggcacg cggctgggca cgttgcccac cagatccatc tgcacggtct
     1861 tgggccacgt gcgatcggcg aatggcagaa acaatcgcgt gagcgccgtc tcggtgtatt
     1921 gcgaaccgat ggccagcatc aggatgacgg ccagcaggat cgaccccagc cccgcggccg
     1981 ttgaatacca caccgggcgg acgacgatgc cgcgcgtcag gtcaacgtgc agcgcaaggt
     2041 tcgtcgtctc ggtcacgatc cgctgcttca tcatctccga gcccggaatg tggccggtga
     2101 tgatgtcgac ggtgctgcgc agccgatcct gaaattgcgg aaacgcctgc tcgagccggc
     2161 ccgcgacgtc gccaagactg agcttgacca gcagcggttg gatcacccag tgccacagcg
     2221 tcagcgccat ggcggcgaag gcgacgagca tcaacaacac ccgcggcacc gcgggcaggt
     2281 taaatgtgta gtcgagcagg atggtgacga
//
LOCUS       DATIMH010000312         4835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_358820, whole genome shotgun sequence.
ACCESSION   DATIMH010000312 DATIMH010000000
VERSION     DATIMH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358820"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VLI90_05310"
     CDS             <1..429
                     /locus_tag="VLI90_05310"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HSV13655.1"
                     /translation="DGTGWEMLCCKPGSRFPIESALVETRDQYMKLKGREVYKFAVTR
                     FEELIEDAMRKCELTREDVSLIIPHQSNLRIIESAMHKLKLPAEKAFVNIDKYGNTSA
                     ASIPIALDEAVRSGRVKSGDTVIFVAFGAGLTWSNAVVRF"
     gene            506..1480
                     /gene="fabD"
                     /locus_tag="VLI90_05315"
     CDS             506..1480
                     /gene="fabD"
                     /locus_tag="VLI90_05315"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HSV13656.1"
                     /translation="MASKTFILCPGQGAQVVGMGKDFYESSPMAKGTFDTANDVLGFD
                     IASLCFNGPEDRLNQTDISQPAIYTTSVAAYRAGLDVGVIDPSVITAFAGLSLGEYTA
                     LFLGGVFTFEDGLKLVAARGRFMQQAAVATPSGMVALLGADEAMADKLCAENAQGEVL
                     VPANYNAPGQIVVSGSRAACDRVIKAAEAAGLKASALKVAGAFHSPLMQSGADKMRAE
                     LDAATFAAPATTVYSNVTAKPHGDPASIKDLLVQQIVSPVRWEQTMRTLITDGGDRAR
                     FVELAPNRHLAGLAKRINRRLPIESLSTVEGLRTKENLVPSPGTPGEG"
     gene            1588..2325
                     /gene="fabG"
                     /locus_tag="VLI90_05320"
     CDS             1588..2325
                     /gene="fabG"
                     /locus_tag="VLI90_05320"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HSV13657.1"
                     /translation="MTEKRVAIVTGGSRGIGAAIVRQLAADGLHVVAIARNLDKLKSV
                     CDEINAAGGSAEPTVCDIADSKTLAETIDQITERHGRVDVLVNNAGITKDGLLLRMDD
                     EDFDSVIDTNLKSAFVTIRSAARMMMRSKTGRIINISSVAGVAGNAGQANYAASKAGL
                     IGLSKSVAKELSGKGITCNVVAPGFITTDMTDVLNDKIKEGVRQFIPLKRFGEPQEIA
                     AVVAFLASPAAGYVTGQVICVDGGMVM"
     gene            2523..2765
                     /gene="acpP"
                     /locus_tag="VLI90_05325"
     CDS             2523..2765
                     /gene="acpP"
                     /locus_tag="VLI90_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSV13658.1"
                     /translation="MAEDIEQKVIEIVSEQMGVDKSEITRETHFINDLNADSLDTVEL
                     VMEFEDEFELSIPDEEAEKIQTVGQAIDYIKEHSSK"
     gene            2875..4140
                     /gene="fabF"
                     /locus_tag="VLI90_05330"
     CDS             2875..4140
                     /gene="fabF"
                     /locus_tag="VLI90_05330"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HSV13659.1"
                     /translation="MHKRRVVITGLGVVTSLGETVDELWENLCAGKSGIKTVTRWDFS
                     KFPVRFGGECTNFDITSYQSEANKYTEEKLVPRRLDRFGQFGIAAALSAVKDSHIDFK
                     KEDPYRCGVIIGSGIGGIETLEEQNKVLIERGVSRVSPFTVPRLMVNAASGNVSIIFG
                     INGPNTAVATACATGSNAIGDAGRYIQFDMADVMIAGGSEAALCELGMGSFIAARALS
                     TRNEEPEKASRPFDKDRDGFVMGEGAGVVILEEYEHAKRRGAHIYAELIGYGMSGDGH
                     HITAPPEDGRGAAKAMQMALADGGITPDKIDYINAHGTSTPLGDVAETRAMKTVFNDH
                     AKKLAVSSTKSQLGHLLGASGGVEAIITALAVERNLLPPTINLDNPDDECDLDYVPHK
                     ARDRKVTYALSNSFGFGGHNATLLLKKPE"
     gene            4354..>4835
                     /locus_tag="VLI90_05335"
     CDS             4354..>4835
                     /locus_tag="VLI90_05335"
                     /inference="COORDINATES: protein motif:HMM:NF023325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HSV13660.1"
                     /translation="MAELVVPSIRTFQVFPDLPQELQPLMELAGNFWWVWHPDAVELF
                     RRLDRKLWDDVYHNPVKLLGQIAQPKLAAAAKDDGYIAHMNRVYTAFRQQLEEPGWFK
                     ETHPDKDKLKVVYFSAEFGLHESLPVYSGGLGILAGDHLKSAAELAIPLTAVGLLYRN
                     G"
BASE COUNT         1030 a         1435 c         1527 g          843 t
ORIGIN      
        1 gacggcacgg gctgggagat gctctgctgc aagccgggca gccggttccc gatcgaatcc
       61 gccctcgtcg agacgcgcga ccagtacatg aagctcaagg gccgcgaggt gtacaagttc
      121 gcggtgacgc gcttcgagga actgatcgaa gacgcgatgc gcaagtgcga gctgacgcgc
      181 gaagacgtgt cgctgatcat cccgcaccag tcgaacctgc ggatcatcga aagcgcgatg
      241 cacaagctga agctgccggc ggaaaaggcg ttcgtgaaca tcgacaaata cggcaacact
      301 tcggcggcgt cgatcccgat cgcgctggac gaagcggtcc gcagcggccg ggtgaaatcg
      361 ggtgacacgg tcatcttcgt cgcgtttgga gcggggctga cctggtcgaa cgcggttgtg
      421 agattttagt agcacgggcg tctcgcccgt gccgagttgc tctgacacgg gcgagagagc
      481 cataggctcg gagcccgtgc tacaaatggc atcaaagact ttcattctct gccccggtca
      541 aggcgcgcaa gttgtcggca tgggcaaaga cttttacgaa tcctcgccga tggcgaaggg
      601 gacgttcgac accgccaacg acgtcctcgg ctttgacatc gcgtcgcttt gcttcaacgg
      661 tcccgaagac cgactgaacc agaccgacat cagccagccg gcgatttaca cgaccagcgt
      721 cgccgcgtat cgcgcgggcc tggatgttgg tgtgatcgat ccatccgtca tcaccgcctt
      781 cgcgggcctg agccttggcg aatacaccgc acttttcctc ggcggcgtct tcacgtttga
      841 agacggtttg aaactcgtcg ccgcccgcgg acggtttatg cagcaagccg ccgtcgccac
      901 gcccagcggg atggtcgcat tgctcggcgc ggacgaagcg atggccgaca agctttgcgc
      961 cgagaacgcc caaggcgaag tgctggtgcc ggcgaactac aacgcgccgg ggcagatcgt
     1021 cgtgagcggc tcgcgagccg cgtgtgatcg cgtgatcaaa gccgccgaag cggcggggct
     1081 gaaggccagc gcgctgaaag tcgccggcgc gtttcatagt ccgttaatgc aaagcggtgc
     1141 ggacaagatg cgggcggaac tggacgccgc cacgttcgcg gcgccggcga cgaccgttta
     1201 ttcgaacgtg acggcgaagc cgcacggcga tccggcgtcg atcaaggacc tgctggtcca
     1261 gcagatcgtc tcgccggtgc gatgggaaca gacgatgcgc acgctgatca ccgatggcgg
     1321 cgaccgggcg cggttcgtcg agctggcgcc gaaccggcat ctggcgggac tggcgaagcg
     1381 tatcaatcgg cggttgccga tcgagagtct ttcgaccgtg gaaggtttgc gcacgaagga
     1441 gaatctggtc ccctcgcccg gtactccggg agagggttag ggtgagggtg attttagaag
     1501 gtcgaaagga cctcgaaatc tcaaatcacc ctcaccccag ccctctcccg gggtaccggg
     1561 agagggggta agacggagat gctaagcatg accgaaaagc gagtcgccat cgtcaccggc
     1621 gggtcccgcg ggatcggggc ggccatcgtt cgccagctcg cggccgacgg cctgcacgtc
     1681 gtcgcgatcg cgcgcaatct cgacaagctc aaatcggtct gcgacgagat caacgccgcc
     1741 ggtggtagcg ctgaaccgac cgtctgcgac atcgccgaca gtaaaacgct cgccgagacg
     1801 atcgaccaga tcaccgagcg gcacggccgg gttgatgtgc tggtgaacaa cgccggcatc
     1861 accaaggatg gattgctgct gcggatggac gacgaagatt tcgactcggt catcgacacg
     1921 aacttgaaga gcgcgttcgt caccatccgc tccgccgccc gcatgatgat gcggagcaag
     1981 acggggcgga tcatcaacat cagttcggtc gccggcgttg caggaaatgc aggccaggcg
     2041 aattatgccg ccagcaaggc ggggttgatc ggcttgagca agtcggtcgc taaggagttg
     2101 agcggcaagg gcatcacgtg caacgtggtg gcgccggggt tcatcacgac ggacatgacg
     2161 gacgtgctga acgacaagat caaggaaggc gtgcggcagt tcatcccgct gaaacgtttc
     2221 ggcgagccac aggaaatcgc ggcggtggtc gcattcctgg ccagccccgc ggcggggtac
     2281 gtgacaggac aggtgatttg cgtcgacggg ggaatggtga tgtgaggagg tcgtcagtag
     2341 tcagttgtcc gttgtcagtt gtggcgatgt gatttgcaag ggcgtgatca caacggacga
     2401 cggaccactg acgactgaca accagtttgc aaaccgcacg gtcacagcta tagtttcgca
     2461 cctcgcgccg tcgcgtggcg gcggtcgagc ggaactacat gacagggcag gtggataaga
     2521 caatggcaga agatatcgag caaaaggtca tcgagatcgt cagtgagcag atgggcgtcg
     2581 ataagagcga gatcacgcgc gagacgcact tcatcaacga tctgaacgct gacagcctgg
     2641 acaccgtaga actggtcatg gagttcgaag acgagttcga gctgtcgatc cccgacgaag
     2701 aggcggaaaa gatccagacc gtcgggcagg cgattgatta catcaaggaa cactccagca
     2761 aatgaaatga gttgctggtt gcttgttgct agttgctagt gcccacgtgt gtgtggcgcc
     2821 agcgtcacgt atctgtacta gcaaccagca actagcaact agcaacttat tctcatgcac
     2881 aaacgccgcg ttgttattac tggcctgggg gtcgtcacct cgctcgggga aaccgtcgac
     2941 gagttgtggg agaacctctg cgccggcaag agcgggatca agaccgtcac ccgctgggac
     3001 ttcagcaagt tcccggtccg cttcggcggc gagtgcacca acttcgacat caccagctat
     3061 caatccgaag ccaacaagta caccgaggag aagctggtcc cgcgccggct ggatcgcttc
     3121 ggtcagttcg gcatcgccgc ggcactgagc gcggtcaaag attcgcacat cgacttcaag
     3181 aaggaagacc cgtatcgctg cggtgtcatc atcggcagcg gcatcggtgg gatcgaaacg
     3241 ctggaagagc agaacaaggt gctgatcgag cgcggcgtga gccgggtgag cccgttcacc
     3301 gtgccgcggc tgatggtcaa cgccgccagc ggaaacgtct cgatcatctt cggcatcaac
     3361 ggtccgaata ccgcggtggc gacggcgtgc gcgactggat cgaacgcgat cggcgacgcc
     3421 gggcgttaca tccagttcga catggctgac gtaatgatcg ccggcggatc tgaagcggcg
     3481 ttgtgcgagt tgggcatggg cagcttcatc gccgcccggg ctctttcgac gcgcaacgaa
     3541 gaaccggaaa aggccagccg tccgttcgac aaggaccgcg acggcttcgt gatgggcgaa
     3601 ggcgcgggcg tcgtcatcct ggaagaatac gaacacgcca agcgccgcgg cgcgcatatc
     3661 tacgccgagt tgatcggtta cggtatgagc ggtgacggcc atcacatcac cgcgccgccg
     3721 gaagacggcc gaggcgccgc caaggcgatg cagatggcgc tggccgacgg cggcatcacg
     3781 ccggataaga tcgactacat caacgcgcac ggcacgagca caccgctggg cgacgtcgcc
     3841 gaaacccgcg caatgaagac ggttttcaac gatcacgcga aaaagctcgc ggtcagctcg
     3901 accaaaagcc aactcggtca cctgctcggc gccagcggcg gcgtcgaagc gatcatcacc
     3961 gcgctggccg tcgagcgcaa cctgctcccg ccgacgatca acctcgacaa ccccgacgac
     4021 gagtgtgacc tcgattacgt ccctcacaag gcgcgcgacc gcaaggtcac ctacgcgctg
     4081 agcaacagct tcggcttcgg cgggcacaac gcgacgctgc tgttgaagaa accagagtga
     4141 cccgagccgc ccatggctca gcatctgttt cttctcgcga atttattccc ttcaaattcc
     4201 aaacgtgcta agccgcaacc ggctcggccg cagaattact ggacctgccg tctgtatcgc
     4261 ctttaccgtc tgggcaaaat agtcgattat acttccagac gcctcgcgcg ttctaacgca
     4321 ctccataaca acgtcgatca acacaggtga aacatggctg agctcgttgt cccctccatc
     4381 cgcacattcc aagtctttcc cgatctaccg caggaacttc aaccgctgat ggaattggcc
     4441 ggcaatttct ggtgggtctg gcatccggac gctgtcgaac tgtttcgccg gctcgaccgc
     4501 aagctgtggg acgacgtgta tcacaacccg gtgaagctgc tcgggcagat cgcccaaccc
     4561 aagctcgccg ctgccgccaa agatgatggc tacatcgccc atatgaatcg cgtgtacacc
     4621 gcgtttcgcc agcagctgga agagccgggc tggttcaagg aaacgcaccc ggacaaggac
     4681 aagctgaagg tcgtctactt ctctgctgag ttcggcctgc acgaatcgct gccggtttat
     4741 tccggcggtc tcggcatcct cgccggtgat catctgaaga gtgccgccga attggccatc
     4801 ccgctcaccg cggtgggcct gctttatcgc aacgg
//
LOCUS       DATIMH010000313         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2667980, whole genome shotgun sequence.
ACCESSION   DATIMH010000313 DATIMH010000000
VERSION     DATIMH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2667980"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1616)
                     /locus_tag="VLI90_05340"
     CDS             complement(123..1616)
                     /locus_tag="VLI90_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV13661.1"
                     /translation="MRFPFAKADDFIAALTFVIAVALILISIVVVQLKQKFSPQLLGA
                     RLKRFFGAELRHLQAHDKNFPGYDLASIHRALLSYFQDHCEPIDTVGSAAPTLSTRDM
                     LQTDDRPYGPWRRKPAPMAYERVAVALDEEESFPSNCLYFAKSKTDPAEKFAVLLSYI
                     RAYRAESGEDMDERSAPASVLTLSIACRRRELADAFFREIESRRQRLSVYRGKVIDPV
                     VTAGGIVTIVFRNIARVGENDLVLPKAVRELIRGSIVNFYTHQTALRALGVEMKRGVL
                     FNSPPGTGKTSVSLYLAGLLPNFTICFVSGRRLLYPREVCAMARYLQPTMLVFEDIDL
                     IAQERETNGLATVLGELMNQIDGCEPNDQVLFIMNTNSMERLEAAVRNRPGRVDQIIH
                     IPLPDRDDRRRLIHHFASTVRVSPAAVVALVDATEGVTPAILKEVIKRAAVMAVQRLA
                     SDGDGRTGGEIDLTTADLLLSVEQVLALRSDMHKLDPLARDPIRFGS"
     gene            complement(1924..>2444)
                     /locus_tag="VLI90_05345"
     CDS             complement(1924..>2444)
                     /locus_tag="VLI90_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HSV13662.1"
                     /translation="WQLGTVQVDYQLPKRFDLSYIGADNKPHPPVMIHRAPFGSMERF
                     VAVLIEHFAGAFPLWLAPVQVAVLSISEKSEEYANRVAQQLKDAGLRVEANTGAEKIG
                     AKIRAATLDKTPYMLVVGEQEAAAGKVAVRHRTAGDQGTMALQDFVERAKQEVASRAQ
                     SAAAPESPAPAR"
BASE COUNT          438 a          766 c          783 g          457 t
ORIGIN      
        1 tttgcatgtt ttttgtcaaa tcgcagccgc ggggttttgg cccgcggacc attcggagtc
       61 gaacgcgtct ggatatccca acggtccgcg ggctaaaagc cccccggctg cgatggggaa
      121 atttacgacc caaaccgaat cgggtcgcgc gccagcgggt cgagcttgtg catgtcgctt
      181 cgcagcgcca gcacctgctc gaccgacaac agcaggtcag ccgtcgtcag atcgatctca
      241 ccaccggtgc ggccgtcgcc atcgctggct agccgctgta ccgccatcac cgcggctcgt
      301 ttgatgactt ccttcaggat cgccggggtc acgccttcgg tggcgtccac cagcgcaacg
      361 accgccgcgg gggaaacgcg gacggtgctc gcgaagtggt ggatgagccg cctgcgatcg
      421 tcgcggtccg gcagcgggat gtggatgatt tgatcaacgc gcccggggcg attccgcacc
      481 gcggcttcca gtcgttccat cgagttggtg ttcatgatga acaggacttg gtcattgggc
      541 tcgcagccgt caatctggtt catcaactcg ccgagcacgg tcgccagccc attcgtctcg
      601 cgctcttgcg cgatcaagtc gatgtcttcg aagacgagca tcgttggctg aagatagcgc
      661 gccatcgcgc acacctcgcg cgggtacaaa agccggcggc cgctgacgaa gcagattgta
      721 aagttcggca gcaagcccgc gagataaagg ctgaccgacg tcttgcccgt gcccggcggg
      781 ctgttgaaca gcacgccgcg tttcatctcg acgccgagcg cgcgcagcgc ggtctggtgc
      841 gtgtagaagt tgacgatcga accacggatc aattcgcgca cggccttcgg cagcacgagg
      901 tcgttttccc cgacgcgcgc gatgttgcga aagacgatcg tcacgatacc gcccgccgtg
      961 acgaccgggt cgatgacctt cccgcgatag acgctgaggc gctggcggcg cgattcgatc
     1021 tcgcggaaga acgcatccgc cagctcgcgc cgccggcagg cgatgctgag cgtcagcacg
     1081 ctcgcggggg ccgaacgctc gtccatgtct tcgccgctct cggcgcggta cgccctgatg
     1141 tacgacagca gcaccgcgaa cttctccgcc gggtcggttt tgcttttcgc gaagtagagg
     1201 cagttggacg gaaacgattc ctcttcgtcg agcgcgacgg ccacgcgttc atacgccatc
     1261 ggcgcgggct tgcgccgcca ggggccgtag ggtcgatcgt cggtctgcag catgtcgcgc
     1321 gtcgaaagcg tcggcgccgc cgagccgacg gtgtcgatgg gctcacaatg gtcttggaag
     1381 taactgagca gcgcccggtg gatggaggcc agatcgtagc cggggaagtt cttgtcgtgg
     1441 gcctggagat ggcggagctc agcgccgaag aagcgcttca gccgtgcgcc cagcagctgc
     1501 ggcgagaact tctgcttgag ctggacgacg acgatcgaga tcaggatgag cgcgaccgcg
     1561 atgacaaacg tgagggccgc gatgaagtcg tccgctttgg cgaagggaaa ccgcacgggg
     1621 atatattagc agagcgccta gagaagtaga gaatctgccc cctctcccgg tactccggga
     1681 gagggctggg gtgagggtga tttcgagtga ggagatccga gtgaggaaat ccgaatgacg
     1741 aagtccgaat ccccgaatca aatccgaatg atgaatgacg aatgacggac acgcgcgcag
     1801 atcgcgcgtt ttcgtcattc ggatttcgtc attcattcgg gaattcggac ttcgtcattc
     1861 gtcattcgaa aatcaccctc accctaaccc tctcccggcg taccgggaga gggaataaga
     1921 gcgtcaccgt gccggcgctg gcgattcggg cgctgccgcg gattgcgcac ggctcgcgac
     1981 ctcctgcttg gcccgctcga caaaatcctg cagcgccatc gtcccctggt cccccgccgt
     2041 tcgatgccgc accgcgacct tgccggcggc ggcttcctgt tcgccgacga ccagcatgta
     2101 tggcgtcttg tccaacgtcg cagcgcgaat cttcgcgccg atcttttccg cgccggtgtt
     2161 ggcttcgact cgcagccccg cgtccttcag ttgctgcgcg actcgattgg catactcctc
     2221 ggacttctcg ctgatgctca gcaccgccac ctgcacgggc gcgagccaca gcgggaacgc
     2281 gccggcgaag tgctcgatca gcacggccac aaaccgctcc atgctgccga agggcgcgcg
     2341 gtggatcatc accggcgggt gcggtttgtt gtcggcgccg atgtagctca ggtcgaaacg
     2401 cttgggcagt tggtaatcca cctgcacggt gccgagctgc cact
//
LOCUS       DATIMH010000314         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_936183, whole genome shotgun sequence.
ACCESSION   DATIMH010000314 DATIMH010000000
VERSION     DATIMH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936183"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1423
                     /locus_tag="VLI90_05350"
     CDS             164..1423
                     /locus_tag="VLI90_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13663.1"
                     /translation="MNARPLARVVVLAISLAAVFPLAGCQSHSQNQSNPPLEAHAPAG
                     PSSAPNVSAQQSSSSSQANNEPAPGGLPDALAQKTAAYARNLEPADQPAAVRRAKQPS
                     PVQWEDPHKPTAAANDHAELAATPPSAAAAKPSPSPSASSDAHPAAAVVQPGDDHAAA
                     IARSAKANALNDIPAIVPESSDFASAGSPANSDPLEQQLIKRAHDNPRDIASQLDCEL
                     FEMLRDEASPQLASLASLPNEDKEVVAAVVDGLTNFRTGVRQDNNMLLSKKIRPLMDM
                     ADRLRSHAELSVPTVALCKRVDGFGKYEPIDPPRFPAGKDNPVIVYCEIQNFESQLNE
                     RRLWQTRLTQQVTLFTETGMLVWNDKSRPVADECRNRRHDFFLYNVVKLPANLTIGRY
                     LLKVSIEDRTAHRVAESTVPVEIVGQQ"
     gene            complement(1572..2573)
                     /locus_tag="VLI90_05355"
     CDS             complement(1572..2573)
                     /locus_tag="VLI90_05355"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HSV13664.1"
                     /translation="MNRRDFIIRTGAAAVGLSLARFTSRQAFAAANAKLRKVLFFTKS
                     SNFEHTVIKRKNGEPSYVEKVLSDLGPKRGIDFTYSKDGSVFTPDYLAQFDGYMFYTS
                     GELTAAGKDRNPPMTPAGKAAFLDAIKNGKGFVGVHSATDTFHTGETAATDTNVARTW
                     RYRNVGDQADPYVRMIGAEFIIHSVQQAATMRIVDPKFPGFGGHGDNVKMMDEWYSLT
                     DFSKDLHVLLVQETAGMTGIPYKRPPYPATWARMHGHGRVFYSSMGHREDTWTNPIFQ
                     DMLFGGLSWTTGVLDADVTPNIEQVTPEAWSLPPVSHPVASDPAKYQADKELVPATK"
BASE COUNT          457 a          785 c          906 g          502 t
ORIGIN      
        1 cgggtcgtcg gagtacctcc gaacccagcg tgggcgattt cacagtctac tatggtccgc
       61 aaagtcatcg cttgctctgc cgcgtctgcc ggttatattg cggttgtccg atatctcgcc
      121 ggtcaaggat gactggcgtg cgcgattcaa ggatgaatca ctcatgaacg ctcgccctct
      181 cgctcgggtc gtggtcctcg cgatttcact ggcggcggtt ttcccgctgg ccggttgcca
      241 atcccactca cagaatcagt cgaatccacc gcttgaagcg cacgcgccgg ccgggccgtc
      301 gtcggcgccg aacgtgagcg ctcagcagtc gtcgtcgtcg tcgcaggcga acaatgaacc
      361 cgcgcccggc gggttgcccg atgcgctggc gcagaagact gcggcgtacg cgcgcaacct
      421 ggaaccggcc gatcaacccg cagccgtgcg tcgcgccaag caaccgtcac cggtgcagtg
      481 ggaggacccg cacaagccga cggcagcggc gaacgatcac gcggagcttg cagcgactcc
      541 gccgtcggcc gcggcggcga agccgtcgcc gtcgcccagt gcatcatccg acgcccatcc
      601 cgcggcggcg gtcgtccagc cgggtgatga tcacgccgcc gcgatcgcgc gatcggccaa
      661 ggccaacgcg ctgaatgaca ttccggcgat cgtgcccgag tcgtccgact tcgccagcgc
      721 gggctcgccg gccaactcgg atccgctgga acaacagttg attaagcgag cccacgacaa
      781 cccgcgcgat attgcgagcc agctggattg cgagctgttc gagatgctgc gcgatgaggc
      841 atcgccgcag ttggcgtcgc tggcgtcgtt gccgaatgag gacaaggaag tcgtcgccgc
      901 cgtcgtcgat ggtttgacca acttccgcac gggcgttcgg caggacaaca acatgctgtt
      961 gtcgaagaag atccgcccgc tgatggacat ggcagaccgg ctgcgcagcc acgcggaact
     1021 gtcggtgccg acggtcgcgc tgtgcaagcg cgtggacggc ttcggcaagt acgagccgat
     1081 cgatccgcct cgcttccccg cggggaagga caacccggtc atcgtgtatt gcgagatcca
     1141 gaacttcgag tcgcagttga acgaacgccg gctgtggcaa acgcggctga cgcagcaagt
     1201 gacgctgttc accgagaccg ggatgctggt gtggaacgac aagagccggc cggtggccga
     1261 tgaatgccgc aaccgcaggc acgatttctt cctgtacaac gtggtgaagt tgccggcgaa
     1321 cctgacgatc gggcgatatc tgctgaaggt gtcgatcgaa gaccgcaccg cccaccgcgt
     1381 ggcggaatcc acggtgccgg tggagattgt cgggcagcag tgaggcgttt gcaccaagtc
     1441 ctggctgcgc gctccggatg accagaacga cgaagcaccg catgtacatg cggggctctg
     1501 cggatgcacc gttacttact tctcggcgag atccatcgat ttcgcacgag cacgcgttgt
     1561 cattgcggtg atcactttgt ggctggcacg agttccttgt cggcttgata cttcgcgggg
     1621 tcgcttgcga ccgggtgtga aaccggcggc aggctccacg cctcgggcgt cacctgctcg
     1681 atgttgggcg tgacgtcggc gtccaggacg ccggtggtcc aactcaggcc gccgaagagc
     1741 atgtcctgga agatcgggtt ggtccacgtg tcttcgcggt ggcccatcga gctgtagaac
     1801 acccggccgt ggccgtgcat tcgggcccac gtcgccggat agggcgggcg cttgtagggg
     1861 atgccggtca tgccggcggt ttcctggacc aacaggacgt gcaggtcttt tgagaagtcg
     1921 gtcagggaat accactcgtc catcattttg acgttgtcgc cgtgaccacc gaagccgggg
     1981 aacttcggat cgacgatgcg catcgtcgcg gcctgctgca cgctgtggat gatgaattcg
     2041 gcgccgatca tgcggacgta ggggtcggcc tggtcaccga cattgcgata gcgccaggtg
     2101 cgggcgacgt tggtgtcggt ggcggcggtt tcgccggtgt ggaaggtgtc ggtggccgag
     2161 tgcacgccga cgaaaccttt gccgttcttg atggcgtcga ggaaggcggc tttgccggcg
     2221 ggggtcatcg gtggattgcg gtccttgccg gcggcggtga gttcgccgct ggtgtagaac
     2281 atgtagccgt cgaactgcgc gaggtaatcg ggggtgaaga cgctgccgtc cttggagtaa
     2341 gtgaagtcga tgccgcgctt ggggccaaga tcgctgagga ccttttcgac gtagcttggc
     2401 tcgccgttct tgcgcttgat gacggtgtgt tcgaagttgc tcgacttggt gaagaagagc
     2461 accttgcgca gcttggcgtt ggcggcggcg aaggcttgcc gcgacgtgaa gcgagcgagg
     2521 ctgagaccta cagcagcggc gccggttcgg atgatgaaat cgcgtcgatt catggtggtt
     2581 gcttttcaaa acgccgcttg cggcttcgca gaccaggcgg cttggattgc gaagccgcaa
     2641 gcggcgggtt
//
LOCUS       DATIMH010000315         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4400545, whole genome shotgun sequence.
ACCESSION   DATIMH010000315 DATIMH010000000
VERSION     DATIMH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4400545"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1130)
                     /locus_tag="VLI90_05360"
     CDS             complement(276..1130)
                     /locus_tag="VLI90_05360"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSV13665.1"
                     /translation="MPSTTIRIFLLAAFCCLASGCAVNNQTEHQQQNNYTSVSATFHP
                     GGTNQSPTNESNNNGGSIMATIGKGLASPFVYIGQQIDYASGHTPAKAVAHMEDKSNA
                     DWRRLGIIDLVSSWDYARKPPYTTRYQQIAQHDPDYTVRAMAIRALNISRDASATPIF
                     IAALDDDNELVRLEATKALANVPDPAAVPALLRHLQGQRETLATAVGRPAASEEAKDV
                     RIAAADALRHYRTLDVARTLVTYLNENEFGVAWQSRQSLRALTGRDLKYDQAAWLNYL
                     TGPEKPFG"
     gene            complement(1158..2141)
                     /gene="murI"
                     /locus_tag="VLI90_05365"
     CDS             complement(1158..2141)
                     /gene="murI"
                     /locus_tag="VLI90_05365"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HSV13666.1"
                     /translation="MFASWSGGCVNHCCRSLRGDCQDYSPERPITRLAVSSPGAPILV
                     LDSGLGGLTVVKALRATLPHEDILYFGDTARVPYGSKTKATVTAFVRQIIAYLRPFNP
                     KHVVIACNTATALAMQSVKAEFTDLTISGVVEPGARAAIEAAGAKASPLIAIIATEAT
                     ARSKAYEHAIHRRRHHARLILRPTPLLVPIIEDGRDENDPLVQLALQQYLKPVMEHRP
                     DVLVLGCTHYPMLKRAITRIAGERCRVIDSAEQCAEDVARRLRTCGLLRGSAAAGAIN
                     GSLRCYVTDDSPKFQTLASRFLGVEIDPPTWVTPEQLIAGPALVLPLRQAG"
     gene            2142..>2424
                     /locus_tag="VLI90_05370"
     CDS             2142..>2424
                     /locus_tag="VLI90_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV13667.1"
                     /translation="MSQPSLEGKRVLLVDDDSDILTSMQAAFEPTGATVETASNGNKA
                     VEIAEKTQPDLVVLDMMLPGRSGFLVLEKIKAKKPRNAKPFVIMITGNQG"
BASE COUNT          439 a          746 c          795 g          444 t
ORIGIN      
        1 aggtcggtcg aacagatgcc ggcctggcgg acgcgcagca gcgtgtcgcc atcgcccgcg
       61 gcggggtcct tgcggcgcgg gtcgaaggcg agcgattcat tgaagcaaag tgcgcgcatt
      121 aatctccccc tcgctcagta cgccgggaga gggctgggga gagggtgatt tgaattcaat
      181 tgacaattga aaatttacaa ttgtcaattt tcaattgaaa aagcaatcac cctcaccctc
      241 tcccggtgtc ccggccgagg ggaccagagg gaatctcaac cgaacggctt ctccggaccc
      301 gttagataat tcaaccatgc cgcctgatcg tatttcaggt cccgccccgt cagcgcgcgc
      361 aaactttgcc gcgactgcca ggcgacgcca aattcgttct cgttcaggta cgtcaccagc
      421 gtgcgggcca cgtcgagcgt gcgatagtgc cgcagcgcat cggcggcggc gatgcggacg
      481 tccttggctt cctcgctggc cgccgggcgg cccaccgcgg tggcgagcgt ttcgcgctgg
      541 ccctgcaggt gccgcagcaa cgccggcacc gccgcggggt ccggcacgtt ggccaacgcc
      601 ttggtggctt cgagccgcac cagttcgttg tcgtcatcca gcgcggcgat gaagatcggc
      661 gtggccgatg cgtcgcgcga gatgttgagc gcgcgaatgg ccattgcgcg caccgtataa
      721 tccggatcgt gctgcgcgat ctgctgatac cgcgtcgtgt acggcggctt tcgcgcgtag
      781 tcccagctgc tcacgaggtc gatgatcccg agccggcgcc agtcggcgtt cgatttgtcc
      841 tccatgtgcg ccaccgcctt ggcgggcgtg tgaccggagg cgtagtcgat ttgctggccg
      901 atgtagacga acggcgacgc cagccctttg ccgatcgtcg ccatgatgct gccgccgtta
      961 ttgttgcttt cgttggtggg cgactgattg gtcccacccg gatggaaggt agcgctcacg
     1021 ctggtgtagt tgttttgctg ctggtgttcc gtctgattgt tcacggcgca gccggacgcc
     1081 aggcagcaga aggcggcgag caggaagatt cggatcgtgg tgctgggcat ctgaatgtgg
     1141 cgcggccgtt cgtcggatca ccccgcctgc cgcagcggta acaccagggc cggcccggca
     1201 atcaactgct cgggtgtgac ccacgtgggc gggtcgatct cgaccccgag gaaccgcgac
     1261 gccagcgtct ggaacttcgg cgaatcgtcc gtcacgtagc agcgaagcga tccgttaatc
     1321 gcgccggccg cggcactgcc gcgaagcagg ccacacgttc gcagccgtcg cgcgacatct
     1381 tcggcgcact gctcggccga gtcaatcacg cggcatcgtt cgccggcgat cctggtgatc
     1441 gcgcgcttca acatcgggta atgcgtgcaa cccagcacca gcacgtcggg gcggtgctcc
     1501 atcaccggct tgagatattg ctgcagcgcc agctggacca gcgggtcatt ctcgtcgcgg
     1561 ccgtcttcga tgatcggcac cagcagcggc gtcggccgca ggatcaaccg cgcgtgatgc
     1621 cgccgacggt ggatcgcgtg ctcataagct tttgatcgag cggtcgcttc ggtggcgatg
     1681 atggcgatca gtggcgacgc cttggccccc gcggcttcga tggcggcccg cgcgcccggc
     1741 tctaccacgc cgctgatggt gaggtcggtg aactccgcct tcacgctctg catcgccagc
     1801 gccgtggcgg tgttgcaggc aatcacaacg tgcttgggat tgaacggccg caggtacgca
     1861 atgatctggc ggacgaacgc ggtgacggtg gccttggtct tcgatccgta tggcacccgg
     1921 gcggtgtcgc cgaaatagag gatgtcctcg tgcgggaggg tggcccgcag ggctttgacg
     1981 acggtcaatc cgccaagtcc gctatccaat acgagaatgg gtgcgcccgg cgaagacact
     2041 gctaagcgcg ttatcggacg ttctgggcta taatcttgac aatcgccgcg gagagatcga
     2101 caacagtgat tcacgcaccc cccggaccaa gaggcaaaca catgagccaa ccaagtttag
     2161 aaggcaagcg cgtcctgctc gtggacgatg acagcgacat cctgaccagt atgcaggccg
     2221 cattcgaacc aacgggcgcg acggtcgaaa ccgcaagcaa cggcaacaag gcggtagaga
     2281 tcgccgaaaa aacccagccg gacctggtgg tgctggacat gatgctgccc gggcgcagcg
     2341 ggtttctggt gctggagaag atcaaagcga agaagccgcg caacgccaag cccttcgtga
     2401 tcatgatcac ggggaaccaa ggcg
//
LOCUS       DATIMH010000316         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3246974, whole genome shotgun sequence.
ACCESSION   DATIMH010000316 DATIMH010000000
VERSION     DATIMH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3246974"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2190)
                     /locus_tag="VLI90_05375"
     CDS             complement(<1..2190)
                     /locus_tag="VLI90_05375"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HSV13668.1"
                     /translation="MHDFCQVSRAPIFAQFGRGRRRLAVRQTRVVSPFCGPNSRKLST
                     EFRRRVLTNRIISMLVAAAGSVAAQSVVITPSSPPVVFRGGTYKFTADRPVRWSLAPG
                     SQGTIDADGTYHASTQVTAKQQAGGCQVLPNDHVFNTKIDTLPVHPSSAAWIAAVGAA
                     APSYGPAFPLNILDSTAATQNLVFAYTSAHNGPFRLPQYPNAKIEGGWFSPPFAGIDR
                     HLIAIDPAACVAEELYNLYPAGTNTYNNCPLCTSQSGVKYSLMTYDLAATGATDAAGM
                     YLEPLTLRVDEIQAALASGGAINHALRFSMSNAVLAKKNVWPAMTYAAVTGQMPYGAR
                     FRLRPGFSATSINPVTQLLLKQLREYGMFAADGGSTWEITVDDTVLIPKTIRDAFVEV
                     HTLVKSSDLEAVDESSLMISPFSGRVNPNNGYVPLDAFATVIATDNANAANIATMAVA
                     LQAVTVGVPRTREFIQAGAGAVQLAAWVNGAADASITWSMSPPLGALTSTGLFTAPAF
                     VPAPAVTVVTAKSKADPTAWAQIEITVLPSGTLRLAVGNSANYLDSQGNTWWGTQYSP
                     IDAGWNYDTGGIWPPIADVALYEMGHYSTGDMRVDVVVPNGNYTLTAKMAETEATSAG
                     QVLQDLEVQGQIAVTGLDVFASAGGRHKPFDVQLPATVTDGRLSFVVRKRGTIGHSKL
                     SSLQILPGNLTTVTVLSSPPGLVLTVDDVPCTSPCGFQWTQGSTHTIA"
BASE COUNT          494 a          775 c          784 g          512 t
ORIGIN      
        1 tgcgatcgta tgagtggagc cctgcgtcca ttggaatccg caaggggacg tgcagggcac
       61 atcatctacg gtgagtacca agcccggcgg ggaactgagc acggtgacgg tcgtcagatt
      121 gccgggcagg atttgaagcg acgacagttt cgaatgtccg atggtaccgc gtttgcgcac
      181 tacgaacgag agcctgccgt cggtgacggt cgccggaagc tgaacatcga acggcttgtg
      241 ccgtcctccc gcggacgcaa acacatccag ccccgtgacg gcaatctggc cttgcacctc
      301 gaggtcctgg agcacctgtc cggcgctggt cgcctcggtt tccgccattt tggccgtcag
      361 ggtgtaattc ccgttcggaa cgaccacgtc gacccgcata tctccggtcg aataatggcc
      421 catttcatac agagcgacat cggcaatggg cggccatatc cccccggtat cgtaattcca
      481 tcccgcatcg atgggcgagt attgcgtgcc ccaccacgtg ttgccttggg agtctaaata
      541 attcgcgctg ttgccgaccg ccagccgcag cgtgcccgaa ggaagcaccg taatctcgat
      601 ctgcgcccac gcggtcggat cggctttgct cttcgccgtg accacggtca cggcgggcgc
      661 cggcacaaac gcgggagcgg taaacaggcc ggtcgaggtc agggcgccca gcggcggcga
      721 cattgaccag gtaatggaag catcggccgc gccattcacc cacgccgcca gttgcacagc
      781 gccggctccg gcctggatga actccctcgt tcgcggcacg cccacagtca cggcttggag
      841 cgcaacggcc atggtggcga tgttagcggc gttggcgttg tcggtcgcga ttacggtggc
      901 gaacgcgtcc agcggcacgt atccgttgtt cggattcacc cgcccggaaa acgggctgat
      961 catcaggctc gactcgtcca cggcctcgag gtcgctggat ttgacgagag tgtgaacctc
     1021 aacgaaggca tctcgaatgg tcttgggaat cagcacggtg tcgtccaccg tgatctccca
     1081 ggttgatccg ccgtcggccg cgaacatccc gtattcgcgg agctgtttga gaagcagctg
     1141 cgtgaccgga tttatcgagg tggcgctgaa gccgggcctc aatcggaaac gcgcgccgta
     1201 gggcatctgt cccgtgaccg ccgcgtatgt catggcgggc cacacgttct ttttcgcaag
     1261 cacggcattg gacatgctga acctgagggc atgattgatc gccccccccg aggccagcgc
     1321 cgcttgaatc tcatccaccc gcagcgtcag cggttcaaga tacatgcccg cggcgtcggt
     1381 ggcgcccgtg gcggcaaggt cgtacgtcat gaggctgtat ttcacgcccg attgcgatgt
     1441 gcacagcggg cagttgttgt acgtgttcgt ccccgcggga tataggttgt acagttcctc
     1501 ggcgacgcag gcggccgggt caatggcgat gagatggcgg tcgatgcccg cgaacggcgg
     1561 cgagaaccag ccgccttcaa tcttcgcgtt cgggtattgc ggaagacgga acggcccgtt
     1621 atgcgcggag gtgtacgcaa acaccaggtt ctgagtcgcc gcggtcgaat cgagaatatt
     1681 cagagggaat gccggaccgt agctcggcgc agccgcgccg actgcggcga tccacgccgc
     1741 cgatgagggg tgaaccggca gagtgtcgat cttggtatta aaaacatgat cgttgggcag
     1801 gacctgacat ccgcccgcct gctgcttggc cgtgacctgc gtgctcgcgt gatacgtccc
     1861 atcggcatca atcgtgcctt ggctccccgg cgcgagcgac caccgcacag gcctgtcggc
     1921 ggtaaattta tatgtgccgc ctcgaaagac tacgggcggg cttgatggcg tgatgacgac
     1981 gctctgtgcg gccacgctcc ccgcggcggc cactaacatc gatatgatcc ggtttgtaag
     2041 cacacgtctc cgaaattcgg tcgataactt tctgctgttg ggtccgcaaa aaggcgacac
     2101 gacgcgggtt tgtctcaccg ccaacctgcg ccgccctcgt ccgaattgtg caaaaatcgg
     2161 cgctcgggaa acctgacaaa aatcatgcac tcttctgtcc gaaaatcgcc ctgaatcata
     2221 gcttgtaaaa taacacacaa ttctaaacta ttttggcccg ccgacacgat gcgggccaat
     2281 caattgcgga tgtacttctt cggcgatatg tctcggcttt gcgacggctg cgaatgcgga
     2341 acacagatat cggaaccgca ggcacgaccg gtacgcgcct gctgaaatgg cgagggtggc
     2401 gccgatcagt tgtgcggcgg attgcgcttt ggcggcgctt cgacgacccg ccacaacatg
     2461 accttttcct agtcgcctca ccacggagta gcgtagtttt ccactgtaac ggtgtgacat
     2521 gcacggtgaa atcagtgtct agccatttga tcccctccgg tttag
//
LOCUS       DATIMH010000317         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1804105, whole genome shotgun sequence.
ACCESSION   DATIMH010000317 DATIMH010000000
VERSION     DATIMH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804105"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1045
                     /locus_tag="VLI90_05380"
     CDS             116..1045
                     /locus_tag="VLI90_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13669.1"
                     /translation="MRMSAGLMIGAWIGLLACTAATGQAAGAPPPPVAPAPVPPAPVP
                     RAGSPEIESQVIPGDQISSISLQDLLNLLKDTVPGFNSVIVRDPEVVPDYPTLPAMTL
                     KNVTVGQLLELIKTSFAGVEISRIDGPTAPLYLIRILPREGVPTQAQAAQAAAMKAAQ
                     PNQAGLAGQFNPNPIVPGFDPASGGPDAPQVKVYRLDDIVRSLTTAESDPASRKKALD
                     DVLSLIQAALDQTGGSDSVVLKVHEPTQTIIFKGSYRKMLVLEQVLETLRPKPNEPGT
                     VEALLRRQRDIEDMVRQIQSRIGKTTGTSTTRP"
     gene            1071..1628
                     /locus_tag="VLI90_05385"
     CDS             1071..1628
                     /locus_tag="VLI90_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV13670.1"
                     /translation="MCDATEKLTAAMAAGDEEAVEVFYRQYFDWLYAQARRASRRDEA
                     FCLDVVQDAVLRVIRVVRPVPSEGQFRAWLRLVVQTTALDRLRAERRRTKRETVVLAA
                     RSADEAVAEEAESFDVAQQQWLREQIAQFDPQLLRMIELRYQQRWTLAKIAGLFGLTT
                     GTVDGRLRRAIRDLRLRAMETFDEQ"
     gene            1618..2046
                     /locus_tag="VLI90_05390"
     CDS             1618..2046
                     /locus_tag="VLI90_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13671.1"
                     /translation="MNSDGGVFLTQEGLQRREQIAAMARAAARARRRRRVQVRMISVS
                     AAVAVCAFTAVTLNDRPRRVTMPGYLPHVAVRNSSPRAPGVIVARIETDPNITTRLAI
                     PRQPPRWRVVGDDDLLQTLTEAGHPAGLVRMNGQAMLLVR"
BASE COUNT          398 a          702 c          752 g          370 t
ORIGIN      
        1 gcgagtacgc tccgaacccg gcgtggtctg gtcgtgctcg cgcaccaatt ctggatttga
       61 cgcgaaaatg cgccggcggc ggcgtatttt cttgcgggac attcaggagc gaaacatgag
      121 aatgagcgcg ggcttgatga tcggcgcgtg gatcggactt ttggcgtgca ccgccgcgac
      181 gggccaggcg gccggcgcac ccccgccccc ggtggcgccg gcgccggtgc cgcccgcgcc
      241 ggtgccgcga gcagggtcgc cggaaatcga atcgcaggtc attcccggcg atcaaatcag
      301 cagcatctcg cttcaggacc tgctcaatct cctgaaggat acggtgcccg gcttcaacag
      361 tgtgatcgtt cgcgatcccg aagttgtgcc tgactatcca acactgccgg cgatgacgct
      421 gaagaacgtg acggttggcc agctgctgga actgatcaag acgtccttcg ccggcgtcga
      481 gatcagccgg atcgacgggc cgactgcccc gctgtatctc attcgaattc tcccgcgtga
      541 aggcgtgcca acgcaggcgc aggccgccca ggccgcggcg atgaaagccg cgcaacccaa
      601 ccaggcgggg ttggcagggc aatttaaccc gaatccgatc gtgccggggt tcgacccagc
      661 cagcggtggg cccgatgccc cgcaggtgaa ggtgtatcgc ctggacgata tcgtgcggtc
      721 actcacgact gcagaatccg atcccgcgag tcggaagaag gcgctcgacg atgtgctttc
      781 gctcattcaa gccgcgctgg atcagacggg cgggtcggac agcgtggtat tgaaggtgca
      841 cgagccgacg cagacgatca tcttcaaggg cagctatcgg aagatgttgg tgctggagca
      901 ggtgctggag acgctgcggc caaaaccgaa tgaaccggga accgtcgaag cgcttctccg
      961 cagacagcgc gacatcgagg acatggtgcg acagatacag tcccggattg gcaagacaac
     1021 cggaacctcg acgacccggc cgtgagaaca cacgatccaa ggcggcgccc ttgtgcgatg
     1081 cgaccgaaaa gctgaccgcg gcgatggcgg ccggtgacga ggaggcggtc gaggtgtttt
     1141 atcggcagta cttcgactgg ctctacgcgc aggcccgccg ggccagtcgg cgtgacgagg
     1201 cgttttgcct ggacgtggtg caggacgcgg tgctgcgggt catccgtgtc gtgcggcccg
     1261 tgccatccga aggacagttc cgcgcctggc tgcgtttggt ggtgcagacg acggcattgg
     1321 atcgccttcg ggctgaacga cgccgaacga agcgcgagac tgtggtgctc gccgcgcgat
     1381 cggcggacga agcggttgct gaagaggcgg agtcgttcga tgtcgcccag cagcaatggc
     1441 tgcgcgagca gatcgcgcag ttcgacccgc agctgctgcg catgatcgag ctgcgttacc
     1501 agcagcgctg gacgctggcg aagatcgctg ggctgttcgg cctgacgacc ggcaccgtcg
     1561 atggccggct ccgccgggcg atccgcgacc ttcgcctgcg cgcgatggag acgttcgatg
     1621 aacagtgacg gcggggtttt tctgacgcaa gagggcctgc aacggcgcga gcagatcgcc
     1681 gcgatggcac gggccgctgc acgcgcccgc cgccggcgtc gcgttcaggt tcgaatgatc
     1741 agcgtgagcg ctgccgtcgc tgtctgcgcg ttcaccgccg tgacgctcaa cgatcggccg
     1801 cggcgggtaa cgatgccggg ctacttacca cacgtcgccg tgcgaaattc atcgccgcgc
     1861 gccccaggcg tgatcgtcgc gcgaattgaa acagatccca acatcaccac tcgcctggcg
     1921 atcccccggc agccgccgcg ttggcgcgtc gtgggcgacg acgacctgct gcaaaccctc
     1981 accgaagccg gccaccccgc gggcctcgtg cgcatgaacg ggcaggcgat gctgctggtg
     2041 cgctagcgcc cgctccggtt ccatctccac cgtcgaactg taaaaagaag acgcccggac
     2101 ctctgggtcc gggcgtctgt tcaatccgaa tgtcgcaaga caatcacgtc gcgtcgctcg
     2161 ccgcctgggc cagctgtggc tggtccggct tggcactctt cagcgcgtcg aagtacaggt
     2221 gc
//
LOCUS       DATIMH010000318         1608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4401412, whole genome shotgun sequence.
ACCESSION   DATIMH010000318 DATIMH010000000
VERSION     DATIMH010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4401412"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VLI90_05395"
     CDS             complement(<1..185)
                     /locus_tag="VLI90_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13672.1"
                     /translation="MCSTRNVHLLICGLLAIALGCEVKQPPKPDAVSHSPLVVDDAMR
                     HRQWPTSVAHYANGQTVA"
     gene            complement(169..468)
                     /locus_tag="VLI90_05400"
     CDS             complement(169..468)
                     /locus_tag="VLI90_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13673.1"
                     /translation="MENGKRKMENVRRRGGGARRVIFCVSICLLPFSICHVPGCAENK
                     PPSTQPTSMEDRQNAAMNDPMNYKPQFGNGDISGGDINHFDRDGFNKDLHDVLNP"
     gene            complement(476..>1608)
                     /locus_tag="VLI90_05405"
     CDS             complement(476..>1608)
                     /locus_tag="VLI90_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HSV13674.1"
                     /translation="PGGRGCESGNGKAPKLVGIMTRRDLKFQEDDNRRIGDVMTKENL
                     VTAPEDTTLDDAERILYRSKVEKLLLVDKENRLSGLITMRDIDKLHQFPNACKDKRGR
                     LRVGAATGVKDFKRIEALINADVDVVVVDTAHGHSKNVLETVREIKKNWTIQVIAGNV
                     ATAEGAKDLIEAGVDAVKIGIGPGSICTTRIVSGVGVPQVSAIMQAVSVGEPAGVPII
                     ADGGIRHSGDITKAIAAGASAVMMGSLFAGLDESPGELVIHQGRRYKTYRGMGSTGAM
                     IQGSKDRYGQAGVKESSKLVPEGVEGRVPYRGPLGDFVYQMVGGLRAGMGYCGTKDIE
                     QLRKNGRFCRISAASMAESHPHDITITKESPNYTVEYAADVK"
BASE COUNT          267 a          552 c          446 g          343 t
ORIGIN      
        1 gcgaccgttt ggccattcgc gtaatgcgca acggacgtcg gccactgccg gtgccgcatc
       61 gcgtcgtcca ccaccagcgg cgagtggctc accgcgtccg gcttgggcgg ttgcttcact
      121 tcgcagccaa gcgcgatcgc cagcaatccg cagatcaata gatgaacatt acgggttgag
      181 cacatcgtgc aaatccttgt tgaagccgtc gcggtcgaag tgattgatgt caccaccgga
      241 gatgtcgccg ttgccgaact gcggcttgta gttcatcggg tcgttcatcg ccgcgttctg
      301 gcgatcttcc atcgacgtcg gctgcgtgct gggcggcttg ttctcggcgc agccgggaac
      361 atggcagatg gaaaacggca aaaggcaaat ggagacgcag aagatgacgc gccgggcacc
      421 gccgccgcgc ctccgcacat tctccatttt ccgttttcca ttttccatgc gaacctcact
      481 tcacgtccgc cgcgtattcc accgtgtaat tcggcgactc cttcgtgatc gtaatatcgt
      541 gcggatggct ctcggccatg ctggcagcgc tgatccggca gaaccggccg ttcttccgca
      601 actgctcgat atccttcgtc ccgcagtaac ccatgccagc ccgcaggccg cccaccatct
      661 ggtacacaaa atcgccaagc ggcccgcgat aaggcacccg gccttccacg ccttcgggca
      721 ccagcttgct gctctccttc acgcccgcct gcccgtagcg gtccttcgag ccttggatca
      781 tcgcgccagt cgatcccatg ccgcggtacg ttttataccg ccggccctgg tgaatcacca
      841 attcaccggg actttcatcg agcccggcaa acagtgatcc catcatcacc gcgctcgccc
      901 ccgcggcgat cgccttggtg atgtcgccac tatgacgaat gccgccatca gcgatgatcg
      961 gcacgcccgc cggttcgccg acgctgaccg cctgcatgat cgccgacacc tgcggcacac
     1021 ccacgccgct gacgatgcgg gtggtgcaaa tcgaacctgg cccgatcccg atttttaccg
     1081 cgtccacgcc ggcctcgatg agatctttgg cgccttccgc cgtggccacg ttgccggcga
     1141 tgacctggat ggtccagttc ttcttgatct cccgcacggt ctccaagacg ttcttgctgt
     1201 ggccgtgcgc ggtatcgacg acgacgacat cgacgtcagc attgatgagg gcttcgatgc
     1261 gcttgaagtc tttgacgccg gtggcggcgc cgacgcggag ccggccgcgc ttgtctttgc
     1321 aggcgttggg gaactggtgc agcttatcga tgtcgcgcat cgtgatcagg cccgacaggc
     1381 ggttctcctt gtccaccagc agcagctttt ctaccttgct gcggtacagg atccgctcgg
     1441 cgtcatccag cgtcgtatct tccggcgcgg tgaccaggtt ttccttggtc atcacgtcgc
     1501 cgatccggcg attatcatct tcctgaaatt tcaaatcccg ccgggtcatg atgcccacca
     1561 acttgggcgc cttgccgttc cccgactcac acccccgccc ccccgggt
//
LOCUS       DATIMH010000319         3610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1228123, whole genome shotgun sequence.
ACCESSION   DATIMH010000319 DATIMH010000000
VERSION     DATIMH010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228123"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VLI90_05410"
     CDS             complement(<1..811)
                     /locus_tag="VLI90_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV13675.1"
                     /translation="MPKATINVCLIGQKFMGRAHSNAYLKAPRFFELPAAPVMHTIVA
                     RNKAEIETFAARWGWQHASIDWRKVIADPAIDLVDVTTPNNKHREIVIAALEAGKHVA
                     CEKPLAATLEDARAMRDAAKRAPSSKTFVWYSYRRVPAVALAHQLVKEGRIGRIYHVR
                     AYYLQDWAGPEIPLVWRFSKEQAGSGSHGDLGAHIIDMARFITGEEITEVIGAAQDTF
                     IKERVLVTEEAKGGISGGATAAGGRKGKVNVDDHTTFMARMKGGAIATFEAT"
     gene            complement(1112..1381)
                     /locus_tag="VLI90_05415"
     CDS             complement(1112..1381)
                     /locus_tag="VLI90_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13676.1"
                     /translation="MNEQEFQTKLAELMGEISTLPSTERQKLEKLADETRQRHERLRQ
                     TVSSLQESLDYLRLSIKYLVFDLEATRRENGYLRKMLEETSGNND"
     gene            complement(1622..2524)
                     /gene="amrB"
                     /locus_tag="VLI90_05420"
     CDS             complement(1622..2524)
                     /gene="amrB"
                     /locus_tag="VLI90_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HSV13677.1"
                     /translation="MAGPSVSNVESRREPVFAGRFYPADDAECQRAAESFVKNAEVGG
                     NDRTRWIGGLVPHAGWICSAAIAGQTIAAMAAGTTPDVVIVFGAIHTPVPTPVAALDV
                     HRKWHVPGADLEVAEFLHGKLSELKTLFTVDERFHAREHAVEVELPLIRAAWPNAMVL
                     PVEVPVREDAIAMGQRAAQQVLSSGLNAVYLASSDLTHYGPSYGFTPAGIGASALQWA
                     KDNDRRLLQLVTDMRVEQIVPEVRARMNACGGGAIAAMMAAAREHGAGNGRLLGHTNS
                     FETLANIHPQPPTDAVGYASLVIA"
     gene            2674..3351
                     /locus_tag="VLI90_05425"
     CDS             2674..3351
                     /locus_tag="VLI90_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13678.1"
                     /translation="MAQPDPDELADALSKLAGGEIAPSEQEPPAPIQQPQAQPARSPA
                     PSAAKPAAKPAARPAAAGAARPQRPTTPAPRPAAPTKAEPLPPSVSAPAETPLDVNLS
                     SEAGMVLPPEQPEIIDDDDSVNIPAPDATVFAPRPRTRPALRTGRSVIYQTIEFRRTI
                     IPILLTCGTLVILFGSLKYTLGPDSALAELPSWLPVVLFITGAVLIALAVVNMLSVKH
                     QLADVKK"
     gene            3509..>3610
                     /locus_tag="VLI90_05430"
     CDS             3509..>3610
                     /locus_tag="VLI90_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSV13679.1"
                     /translation="MASENVKEFTDQNFETEVLNSQQPVLVDFWAEWC"
BASE COUNT          623 a         1234 c         1074 g          679 t
ORIGIN      
        1 gcgtggcctc gaaggtggca atcgcgccgc ctttcatgcg ggccatgaaa gtggtgtggt
       61 catcgacgtt gacctttccc ttgcgtcccc ccgccgccgt cgccccaccg gagatgccgc
      121 ccttggcttc ttccgtgacc aagacgcgct ccttgatgaa cgtgtcttgg gcagcgccga
      181 tcacctcggt gatttcctcg ccggtgatga accgggccat gtcgatgatg tgcgcgccca
      241 agtcgccatg gctgccgctg ccggcttgct ccttgctgaa gcgccagacc agcggaattt
      301 ctggtcccgc ccagtcctgc aggtaatacg cgcgcacgtg atagatccgc ccgatgcgac
      361 cttctttcac gagctgatgc gccagcgcca ccgccggcac gcggcggtag ctgtaccaga
      421 cgaacgtctt gctgctcggc gctcgcttcg ctgcgtcgcg catcgcgcgg gcatcttcca
      481 gcgtcgcggc caacggcttt tcgcaggcga cgtgcttccc cgcttccaac gcggcgatga
      541 cgatctcgcg gtgcttattg ttcggcgtcg tcacatcgac cagatcgatc gcgggatcgg
      601 cgatcacctt gcgccaatcg atgctggcat gttgccagcc ccatcgcgcg gcgaacgtct
      661 cgatctccgc cttgttgcgc gcgacgatcg tgtgcatgac cggcgcggct ggcagctcga
      721 agaatcgcgg cgccttcagg tatgcattgc tgtgcgcccg gcccatgaac ttctggccga
      781 tgaggcagac gttgatcgtc gctttaggca tctggtttcg gaacctctcg cgggggcggg
      841 cgcgctcgct tcagagacgt ttcagtgacc gcgcgaagga gtttaacggt cgcgcccgac
      901 catgcaagcg gggaccaacg cggcgacagc gccgaggcac ttcgatcctt cgcgtagcga
      961 tagcctcgcg ggggcacgtg tttttcttcc gatcgaaaaa cgtgtgcccc tgcgaggcta
     1021 ccgctacgca tgaagaagaa cgcgaaaaaa acggacgccg cgcaagtgcg cggcgtccgc
     1081 ctcagtatca attcgtgcag cgttcccgtc tttagtcgtt attcccgctc gtctcctcca
     1141 gcatcttgcg caggtacccg ttctcgcggc gcgtcgcttc gagatcgaac accaagtact
     1201 tgatactcag ccgaaggtaa tccaggcttt cctggaggct gctgaccgtc tgccgcaggc
     1261 gctcgtggcg ctggcgggtt tcgtcggcaa gcttctccag cttttgccgc tcggtcgatg
     1321 ggagcgtgct gatctcgccc atcaattccg ccagcttggt ttggaactcc tgctcgttca
     1381 tgtccgtctc ctgaaacgaa aaactccgtg ggtgcctcgc tcgttgaacc actgaggcaa
     1441 agcccgttcc acatccgtgc aggggtacga ctccgtccaa accgtcaaac cattcgagtg
     1501 aattcgatcc gttccgcccg ttccgcccgc gccgcgtgcg cgggatgtcg cccgcgggca
     1561 tcattcctcc acctgggtgc ttcgtcgttg cgacattcgc aatggatgtt gcgttgacgc
     1621 atcacgcgat caccagcgat gcatagccca ccgcgtccgt cggcggctgc gggtgaatgt
     1681 tcgccaacgt ctcgaaacta ttcgtgtgcc cgagcaaccg cccattcccg gcgccatgtt
     1741 ctcgcgccgc ggccatcatc gcggcgatcg ccccgccgcc gcaggcgttc atccgcgccc
     1801 gcacctccgg cacgatctgc tcaacccgca tatccgtcac cagctgcaac agccggcgat
     1861 cgttgtcctt cgcccactgc aacgccgacg ccccgatgcc cgcgggggtg aagccgtagc
     1921 tggggccata gtgcgtcagg tcgctgctcg ccaggtacac cgcgttcaat ccgctgctca
     1981 agacctgctg cgcggcgcgc tgtcccatcg caatcgcatc ttcgcgcacc ggcacttcca
     2041 ccggcagcac cattgcattg ggccaagccg cgcggatcag tggcagctcc acttccaccg
     2101 cgtgctcgcg cgcgtgaaat cgttcgtcaa ccgtgaacag cgttttcaac tcgctcaact
     2161 tgccgtgcag aaactcggcc acctcaagat ccgcccccgg cacgtgccat ttgcgatgca
     2221 catccaaggc agccaccggt gtcggcacgg gcgtgtgaat cgcgccgaag acaatcacga
     2281 catccggcgt ggtccccgcg gccatggcgg cgatcgtttg acccgcgatg gcggcgctgc
     2341 agatccaccc cgcgtgcggg acgaggccgc cgatccatcg cgtgcgatcg ttcccaccga
     2401 cttccgcgtt cttcacgaac gactcggcgg cgcgctggca ctcggcgtcg tccgcgggat
     2461 aaaaccgccc ggcgaagacc ggctccctcc gggactcgac gtttgacacg cttggcccgg
     2521 ccatgcgcgg cactataatc ccgcccggaa ataccggaca atgtgaaatg cgcccgtgac
     2581 aggagaaaga gttatcggtc cgttgaacac gggcaacttg caacggtcct gtcgatcgtt
     2641 cgtgccgtgc gcgcgccccc ggagcgatgt gtgatggccc aacctgatcc tgatgaactg
     2701 gccgacgccc tgtccaagct cgccggcggc gaaatcgcgc cgagcgaaca ggaaccgccg
     2761 gcgccaatac agcaaccgca ggctcagccg gcgagatcac cggcgccatc cgcggcaaaa
     2821 cccgcggcaa agcccgccgc tcgacccgcc gctgcagggg cagcgcgccc gcagcggccg
     2881 acgacgcctg cgccgcgacc ggcggcgccg acaaaggcgg agccgctgcc gccgagcgtc
     2941 tccgcacccg ccgaaacgcc gctggatgtg aacctctcga gcgaagccgg catggtcctg
     3001 ccgccggaac agccggagat catcgatgat gacgattcgg tgaacattcc cgcgccggat
     3061 gcgaccgtct tcgcgccgcg gccgcgcacg cggccagcgc tgcgaacagg tcggtcggtc
     3121 atctaccaga ccatcgaatt ccgccgcacg atcatcccca tcctgctgac ctgcggcacg
     3181 ctcgtgatcc tcttcggttc gctgaagtac acactcggcc cggactcggc cttagcagaa
     3241 ttgccgtcgt ggctgccggt cgtgctgttc atcaccggcg cagtcctgat tgcgctggcg
     3301 gtggtgaaca tgctgagcgt gaaacatcag ctggcggatg tgaagaagtg aacgcggcac
     3361 tcgcgtcact cgttaatagc cgcaagcgag aagccagcgg ctggcaaagc acttgcgccg
     3421 cccgcgttga caatgcttgc ggtggacttc tatcattcgc gtcgaatcgc acatacgcag
     3481 cgatcgcata gttcgaagga gcttgtttat ggccagtgag aacgtcaagg aatttaccga
     3541 tcagaatttt gaaaccgaag tgctcaacag ccagcaaccg gtgctggtgg atttctgggc
     3601 cgagtggtgc
//
LOCUS       DATIMH010000320         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3824335, whole genome shotgun sequence.
ACCESSION   DATIMH010000320 DATIMH010000000
VERSION     DATIMH010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3824335"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VLI90_05435"
     CDS             complement(<1..656)
                     /locus_tag="VLI90_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HSV13680.1"
                     /translation="MTSKDLLKLGFKPSRSLGVALRLLPELSKWFDDEYLKRELKAVL
                     QDPIRNAAHSQFSEVALAIREEQEAPPYVERPVPAPYQIWGENLEPTAVDQIKAASRL
                     PVAVRGALMPDAHLGYGLPIGGVLATENAVIPYAVGVDIACRMKLSVYDIPADDLARL
                     DAQLTKALQRETLFGTGKSFKQPYDHEVLEADWRVSPITAKNFDKARGQLGSSGSGNH
                     "
     gene            complement(732..1802)
                     /locus_tag="VLI90_05440"
     CDS             complement(732..1802)
                     /locus_tag="VLI90_05440"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSV13681.1"
                     /translation="MALQHEQVMAALRTVRDPELFRDIVTLGMIKGVKIDGATVWIHL
                     ELTTPACPMKDAIERDVRAALTQIGAEHVEMNTTANTRGPAQPRADVLPQVKNVIAVG
                     AGKGGVGKSTVALNVAVCLSRTGARVGLMDGDIYGPSMPTMLGIKGLPPQVRGSKLQP
                     FHVHGIHAVTIGALVEQDKPLIWRGPMAHGAFKQLLLDNTEWPDLDYLIVDLPPGTGD
                     VPLTLCQLLPLTGAVVVATPQQVALDDAIRAVRMFQQLNTTVLGLVENMSYIPLPNGE
                     VLDMFGRGGTQKAAQELGLPYLGAIPMFTELRQNSDAGTPGKNFEVNPALRESLETVV
                     GNLAGEVSKRNMAGAGPTLNVT"
     gene            2179..3462
                     /locus_tag="VLI90_05445"
     CDS             2179..3462
                     /locus_tag="VLI90_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HSV13682.1"
                     /translation="MTTLSTQPTDRTDALNEMPCPPSPHETGTASGQTIRPGANDDGW
                     IVPAPVKLSDGTTIRLYKDGEAWHAAFDAMRDAKMRICLEIYIFASDDTGRAVADFLC
                     KKARDGLRVYVVYDSFGSLETDREMLRQMQRAGVNLQQFHPMLPWECRYSWRPVNRDH
                     RKLIVIDNEVGGLGGINLATEYAGSSIIRALKPKAARMSRCELWRDNAISIRGPSVIH
                     LMRSFARTWNYVLHGGRIRRAELIHNIHQGEFGLLASVPTLSSPLQPFLCNLLRSART
                     KILLTMAYFAPDEVLVNELCKAADRGVHVQLMLPGRGDVKLLVVAARSFYSRLMSHGV
                     DIYERQNVVLHAKTLVIDGHISVVGSTNLDYRSIEYNCELSAIIRSEEFGQQMDDLFQ
                     HDIRFARQIFPEEWRTRPFCDRVGQWAVSRARYLL"
     gene            3477..>3779
                     /locus_tag="VLI90_05450"
     CDS             3477..>3779
                     /locus_tag="VLI90_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13683.1"
                     /translation="MASISQTASNVSKLVRYAMGAAMSPIHAGDEKIASRQPTLNAPK
                     SLTITSNSFLPNQPIPTKHTPLAENLSPTLSWSNVPAEARELVLICEDPDAPMAKPF"
BASE COUNT          698 a         1194 c         1168 g          719 t
ORIGIN      
        1 aaatgattcc cggaaccgct ggagcccagt tgcccgcggg ctttgtcaaa attctttgcg
       61 gtgatcggcg agacccgcca atccgcctcc agcacttcat gatcgtacgg ctgcttgaac
      121 gacttgcccg tgccgaacag cgtttcgcgc tgcagcgctt tggtcagctg ggcgtcaagc
      181 cgagccaggt catccgcggg aatgtcgtag acgctaagct tcatccggca ggcgatgtcg
      241 acgccgacgg cgtaggggat gacggcgttc tcggtcgcca gcacgccgcc gatcggcagg
      301 ccgtaaccga ggtgcgcatc gggcatcagc gccccgcgca ccgccaccgg caggcgcgac
      361 gcggccttga tctgatcgac ggcggtcggc tccaggttct cgccccagat ctgatacggc
      421 gccgggaccg gccgctccac gtacggcggg gcttcctgct cttcacgaat cgccagcgcg
      481 acttcggaaa actgcgaatg cgcggcgttg cggatgggat cttgcagcac cgctttcagt
      541 tcgcgcttga ggtattcgtc gtcgaaccat ttggaaagct cgggcagaag gcgcaatgca
      601 acgccgagcg agcggctcgg tttgaagccg agtttcaaga ggtctttgga tgtcatccat
      661 tcccccgtct ttgccgcttg cggcttcgca atccggcacg tgtcggattg cgaagccgca
      721 agcggcaaca gtcacgtcac gttcaacgtc ggtccggcgc cggccatgtt gcgcttcgaa
      781 acttcaccgg cgagatttcc cacgaccgtc tccaacgatt cgcgcaacgc cgggttcacc
      841 tcaaaatttt tccccggcgt tcccgcgtcg ctgttctgtc gcaactccgt gaacatcggg
      901 atcgcgccga ggtaaggcaa tcccaattcc tgcgccgcct tctgcgtgcc gccgcggccg
      961 aacatgtcca gcacttcgcc attgggcagc gggatgtagc tcatgttctc gaccaggccc
     1021 agcaccgtcg tgttgagttg ttggaacatc cgcaccgcgc ggatcgcgtc atccagcgcc
     1081 acctgctgcg gcgtcgcgac aacgaccgcg cccgtcagcg gcagcaactg gcacagcgtc
     1141 agcggcacgt cgccggtgcc gggcggcagg tcgacgatca ggtaatccaa gtccggccat
     1201 tccgtgttgt cgagcagcag ctgcttaaat gcgccgtggg ccatggggcc gcgccaaatc
     1261 agcggcttgt cctgctcgac cagcgcgccg atcgtcaccg cgtgaatgcc gtgcacgtga
     1321 aacggctgaa gtttcgatcc gcgcacctgc ggcggcaggc ccttgatgcc gagcatcgtc
     1381 ggcatgctgg ggccgtaaat gtcgccgtcc atcaggccga ctctggcgcc ggttcgactg
     1441 aggcagacgg cgacgttcag cgcgacggtg gacttgccga cgccgccctt gccggcgccg
     1501 acggcaatca cgttcttcac ctgcggcagc acatcggccc gcggttgggc gggcccgcgc
     1561 gtgttggcgg tcgtattcat ttccacgtgt tcagctccaa tctgcgtcag cgccgctcgc
     1621 acgtcgcgct cgatcgcgtc cttcatcggg cacgccggcg tggtcaactc gaggtgaatc
     1681 cagacggttg caccgtcgat cttcacaccc ttgatcatgc ccagcgtgac gatgtcgcgg
     1741 aacaactcag ggtcgcgaac cgtccgcaac gccgccatca cctgctcgtg ctgcagtgcc
     1801 ataagtgtcg cgcatcgagc gcgaaccatt gagtatagcg gtactgagcc ccgcatgtac
     1861 atgctgggct tctggagcta ttggttgtcc gcaccttgtt caccgccgag gcgccgagag
     1921 cgccgagagc gccgagcgcc gaggcagacg ccgagaagag aagaacttga gccgcagttt
     1981 atgcagattt cgcggattca gacaaagcct cgtccaaact gcgtaatctg tgaaatctgc
     2041 ggttaaaact cttctgtctt tgtcttctcg gcgtctgcct cggccctctc ggcgcctcgg
     2101 cggtgaacca acccctcgaa gtcgaaaacg aaattccacc gttacgctcg cctgggcgtt
     2161 gcataagatt tgttccagat gaccacgctc agcacgcagc cgacggatcg caccgatgcg
     2221 ctcaatgaga tgccgtgccc gccgtcgccg catgagacgg ggacggcgag cgggcagacg
     2281 attcggcccg gcgcgaacga cgatggttgg atcgtgcccg cgccggtgaa actatccgat
     2341 ggcacgacca tccgcctgta caaggacggc gaagcttggc acgcggcgtt tgacgcgatg
     2401 cgcgacgcga agatgcgcat ctgcctggag atttacatct tcgccagcga tgacaccggc
     2461 cgggccgtcg cggatttcct ctgcaagaaa gctcgcgatg gcctgcgagt gtacgtcgtc
     2521 tacgattcgt tcggatcact cgaaaccgat cgcgagatgc tccggcagat gcagcgcgcc
     2581 ggcgtaaacc tccagcagtt ccacccgatg ctgccgtggg aatgcagata cagctggcgg
     2641 ccggtgaacc gggaccatcg caagctaatc gtgatcgaca acgaagttgg cgggctgggt
     2701 gggatcaacc tggcgaccga gtacgcgggg tcgtcgatca tccgcgcgct caagccgaag
     2761 gccgcccgca tgtctcgctg cgaactttgg cgagacaacg ccatcagcat ccgcgggcca
     2821 tcggtcatcc atctgatgcg atcgttcgcg cgcacgtgga actacgtcct gcacggcggg
     2881 cggattcggc gggcggaatt gattcacaac atccaccagg gcgagttcgg actgctggcg
     2941 agcgtgccga cgttgagcag tccattgcag ccattcttgt gcaacctgct gcgtagcgcg
     3001 cgaacgaaga tcctgctgac gatggcgtat ttcgcgcccg atgaggtgct ggtgaacgag
     3061 ctgtgcaaag ccgccgaccg cggcgtgcac gtgcagctga tgctgccggg gcgtggcgat
     3121 gtgaagctgc tggtggtcgc cgcgcgcagt ttctattcgc gcttgatgtc gcacggcgta
     3181 gacatttacg agcggcagaa cgtcgtgctg cacgccaaga cattggtgat cgacgggcac
     3241 atcagcgtgg tcggatcgac caacctcgac taccgcagca tcgaatataa ctgcgaattg
     3301 tctgccatca tccgctcgga agagttcggc cagcagatgg acgatctttt ccagcacgac
     3361 attcgctttg cccgacaaat ttttcccgaa gagtggcgaa cccgcccgtt ctgcgaccgc
     3421 gtcggtcagt gggcggtcag ccgagcccga tatcttttgt agaaacggag gcttttatgg
     3481 ccagcatctc ccaaacggcg tcgaacgtga gcaagctggt tcgatatgcg atgggggcgg
     3541 cgatgtcacc gatccacgcc ggcgatgaga agatcgcctc gcgccagccg acgctgaatg
     3601 cgccaaagtc actgacgatt acgagcaatt cgttcctgcc gaaccaaccg atcccgacga
     3661 agcacacgcc gctggcggag aatctgtcgc ccacgttgtc gtggtcgaac gtgccggcgg
     3721 aagcgcgcga gctggtgttg atttgtgaag atccagatgc gccgatggcc aagccgttc
//
LOCUS       DATIMH010000321         9579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4401748, whole genome shotgun sequence.
ACCESSION   DATIMH010000321 DATIMH010000000
VERSION     DATIMH010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4401748"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VLI90_05455"
     CDS             <1..804
                     /locus_tag="VLI90_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV13684.1"
                     /translation="DLRIETGSTVGLIGPNGGGKTTLVKLLIGLLKPTRGSITVDGLT
                     PAQAVRRGDVIGYLPQNPGRLEKFPLSVRQVVRLGLSGKTGMLRGIAKDDAEFADFLL
                     QRVGMGELAQTPVGQLSGGQLQRVFIARALAPRPKILLLDEPTTGIDRLGQQQFIDFL
                     QGLKRELGLTVIMVSHDLRAVSAISDRIACLNLTLHYHDVPERLPADLVYRMFACDLQ
                     AMGIGGEHKGCGDPTCDHGQSVARASRPCPEPLDTGSGAVLQKTDQLAQ"
     gene            794..1636
                     /locus_tag="VLI90_05460"
     CDS             794..1636
                     /locus_tag="VLI90_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HSV13685.1"
                     /translation="MPNNPTFLYGLPSAVALAIACAVLSVMVVSRRWAFIGEGISHSG
                     FGGAGTAWVLALLFPAVFDQPWMPYLCVVCFSLLTAIAIGYLSYRQRVNSDAAIGIFM
                     VASLAWGFLGRQIYFAHRHAEPVGFDQFLFGEMGALSAQYAIATVMLSAAIVLIVAVL
                     AKEILYYCFDPVMAEASGVPAGFVHYLLMLLVALAIVLGVRVAGSVLVTALLVLPGAT
                     ALLISERLRTVIAAAVTIAVIGAAGGVGLNLHWRFIPAGPAIVLVLFVEFVLSYVASV
                     VARR"
     gene            complement(1606..2661)
                     /gene="ada"
                     /locus_tag="VLI90_05465"
     CDS             complement(1606..2661)
                     /gene="ada"
                     /locus_tag="VLI90_05465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HSV13686.1"
                     /translation="MKRRATGNDHRWQAVLAHDATQDGRFVYAVRSTGVFCKPSCPSR
                     RPGPEQVEFFETPAMAERAGYRACRRCRPGAESTVDALIKFIDGRTDVPRALNEFAQQ
                     NGVGVQHLRRLLRAKTGMTPGAFDRARRVERLKSNLRNGRSVTHAALAAGFPSPARLY
                     ERADRQLGMTPGCYRDGGRGVHIRYAVVDCSLGKLLVATTAKGVCAVKLGDTERPLVR
                     ELNQEFPAATITPGDVPALAQVLRLLDGRPTIGAVPLDMRGTLFQRTVWAALCAIRPG
                     ETRSYQQLAASVGRPAAARAVARACATNPVAVVVPCHRIVRGTGQISGYRWGVDRKKS
                     LLERERRVQRRATTLAT"
     gene            2828..4234
                     /locus_tag="VLI90_05470"
     CDS             2828..4234
                     /locus_tag="VLI90_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13687.1"
                     /translation="MRSIALTALLSLLLASAASAASVSLTITPEWNNAGRQIPADFLG
                     LSYETKMLLPTRAGQRFFRPDNTPLVSMFKSLGMRNLRIGGNSVDTPSVDVPSEADID
                     SLYAFAAAADVRVIYSVRMKAAVEPTDAARVAKYVMDHYADRTAAITVGNEPNMYFVD
                     YAPYRNDYERFASAILAVAPNAIISGSSATPGKSPWTGELVRDVGGTPMLKLVTQHSY
                     PGGNATKVTNAAHARRILLSPEIDAGYEAFYATFAPAAIEKHLPYRLEEANSFFHGGA
                     PGVSNSFASALWALDYLYWWASHDAAGINFHTNNAIVADEQKIPGGYDTFWTGPNGFK
                     VHPIGYAEKAFTLAAPGGRLVPVKIAGANGVNVVAYATLAADGTRYVTVINRESGEAG
                     RAIAATTGMGDGASNAESLTLAAPNNDEAALTGVTLGGQMIGSAGNWNGRWTESTPDV
                     LVMPTSCVVIRASSVQRR"
     gene            4493..5176
                     /locus_tag="VLI90_05475"
     CDS             4493..5176
                     /locus_tag="VLI90_05475"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSV13688.1"
                     /translation="MAQIFHPSANTIAKASIFGAVFVAAFVGYCAAGYDRSVWHTRAG
                     YAKAQPVPFSHEHHVRGLGIDCRYCHTSVETSHFAGIPSTKTCMTCHSQVWTGAALLE
                     PVRESWRTNRPIEWNRVHNLPEFVYFNHSIHVNKGIGCSSCHGQVDKMPLMWQEGSLQ
                     MEWCLNCHRDPAKFVRPRAEIYNLNYDPAMDPSLPANMRSQETLGPQLVKDYHIHTEQ
                     LTNCSICHR"
     gene            5238..8420
                     /locus_tag="VLI90_05480"
     CDS             5238..8420
                     /locus_tag="VLI90_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HSV13689.1"
                     /translation="MLDACPHHHEDEAEKLDGLTLRTPRTSQGKRMWRSLDELAQTPD
                     FLNMMHREFPHAASEWTDEPSRRNFLKLMSASLALGGLTACTLQKTPEKILPYVNPPE
                     EVIPGRPLFFATSAPWCGYAKGVVVESHEGRPTKIEGNAEHPSTGGYGGTDIWMQASV
                     LDMYDPDRSQTVMHGYGNVSTWGAFTDELSGKMAEQQSKGGAGLRFLTGTVTSPTVAR
                     QIQGILKKFPQAKWHQWEAVGRHNARDGALRAFGQDVQAVYRLDQAKVILSLDSNFLL
                     EDPGSLAYAKQFIDGRRVRRDRDEMNRLYTVESGVTITGAMADHRLALRSSEIESLAQ
                     QLAGAINGTAASGPNAKWVNAVAKDLQANRGHALVLVGESQPASVHALGYAINDALGA
                     AGSTVVYVDPIETSGPDGPHTLEELVSDINGGTIDLLVILGTNPSFTAYSDMGFVSGA
                     GEKTPEDRTQAYNVKNPLSKVKTVVHHGTHFDETAFFSHWHIPASHYLESWGDLRSYD
                     GTASIVQPLILPLYSSKSDVELLAFLDGDANRGAYDIVRETWQSQMAAPYEATWAKSL
                     EKGVIDGSAAKPKQVALKTNIGALLGGATTQPATAGGLELVFRPDPHIWDGRFANNGW
                     LQELPKPITLLTWDNAALMGYNTARKLGVIQNDPGRATEASMIEIKHGSTTLRVPALV
                     VPGHAEDAITLHLGYGRSRAGNVGNGKGVNAYDMRTRATPWVVAGVEVNLTGDMFPLA
                     TSQSHHLMEVGRAPEMDKSWMAERELFHVFTRDEFAEKLEEYHKAQGANDARKLRTIE
                     LPLLKHNPDEEAESMLPNWDYSHNKWGMVIDNNACIGCNACVIACQSENNIPVVGKEQ
                     VLRGREMHWLRIDTYWYGKPEEAPDVYFQPITCMHCETAPCTLVCPVEATSISAEDIN
                     EQTYNRCVGTRYCSNNCPYKVRRFNFLYYNDYKTPSLMLQRNPNVTVRSRGIMEKCSY
                     CVQRVNNGRIEAKKQDRLIAPGEVRTACEQSCPTQAIMFGNLNDPRWDATKLQDEPHR
                     YTLLDELNTKPRTSYLSRLKNPNPELVGSA"
     gene            8574..>9579
                     /gene="nrfD"
                     /locus_tag="VLI90_05485"
     CDS             8574..>9579
                     /gene="nrfD"
                     /locus_tag="VLI90_05485"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HSV13690.1"
                     /translation="MADKPLHPHLHTVSTMDDERLVVPPPVVGEGYTIARVSDKIASV
                     VLTHPVTKGWLLGFTLAFTLVMALTMAIAYLFTKGVGIWGINMPVAWGFAIVNFVWWV
                     GIGHAGTFISAFLLLLRQDWRTSINRFAEAMTLFAVACAGMFPLLHVGRPYMAYWLLP
                     YPNTMLLWPQFRSPLVWDVFAVSTYATVSLLFWYVGLIPDLATMRDRAPHWYGRLAYG
                     VFSLGWRGSARHWHRYQAAYILLAGLAAPLVVSVHSVVGMDFAAGQVPGWHSTIFPPY
                     FVAGAIFSGFAMVVTLAIPLRYFYGLRDFITLRHLDNCAKLMLVTGLIVAYGYGIEAF
                     M"
BASE COUNT         1765 a         2953 c         3153 g         1708 t
ORIGIN      
        1 gacctgcgca tcgaaaccgg cagcacggtc ggcctgatcg gtcccaacgg cggggggaag
       61 acgacgctgg tcaagctgct gatcgggctt ttgaagccga cgcgcgggtc gatcaccgtg
      121 gatggtctga cgcccgcgca agccgtgcgg cggggggatg tgattggcta tctgccgcag
      181 aacccggggc ggctggaaaa atttccgctg agcgtgcggc aagtggttcg gctgggcctg
      241 tcgggcaaga cgggcatgtt gcggggaatt gccaaagatg acgcggaatt cgcggatttc
      301 ctgctgcagc gcgtcggcat gggcgaattg gcccaaacgc ctgtcgggca gctgtcgggc
      361 gggcagttgc agcgcgtgtt catcgcccgc gcgctcgcgc cgcggccgaa gatcctgttg
      421 ctcgatgagc cgacgacagg gatcgatcgc ctcgggcagc agcaattcat cgacttcctg
      481 cagggcttga agcgcgagtt gggattgacc gtcatcatgg tcagtcacga tcttcgcgcg
      541 gtgtccgcca tcagcgaccg aatcgcctgc ctgaacctca cgctgcatta ccacgacgtc
      601 cccgagcgcc tccccgccga cttggtctac cgcatgttcg cctgcgacct gcaggcgatg
      661 ggcattggcg gcgaacacaa aggatgcggc gatcccacct gcgaccacgg acaatccgta
      721 gcacgggcgt ctcgcccgtg tcccgaaccg ctcgacacgg gttccggcgc cgtgctacaa
      781 aagaccgatc aacttgccca ataatcccac gtttctctat ggcctcccca gcgccgtcgc
      841 gctcgcgatc gcctgcgcgg tcctcagcgt catggtcgtc tcgcgccgct gggcgttcat
      901 cggcgaaggc atcagccata gcggttttgg cggggccggc accgcgtggg tgctcgcgct
      961 gctctttccc gctgtcttcg atcagccgtg gatgccgtac ctgtgcgtcg tctgtttctc
     1021 gctgctgacc gcgatcgcga ttggctattt gagctaccgc cagcgcgtga acagcgatgc
     1081 cgccatcggc atcttcatgg tcgcgtcgct cgcgtgggga ttcctgggcc ggcagattta
     1141 cttcgcgcat cgccatgccg agccggtcgg cttcgaccaa ttcctcttcg gcgaaatggg
     1201 cgcgctctca gcgcagtacg cgatcgcgac ggtgatgctc agcgccgcca tcgtgctgat
     1261 cgtcgcggtg ctggcgaaag aaatcctcta ctactgcttc gaccccgtga tggccgaagc
     1321 ctccggcgtg cccgcggggt tcgtccacta cctgctgatg ttgctcgttg cactggcgat
     1381 tgtccttggc gtccgcgtcg ctggcagcgt gcttgtgaca gcgctgctgg tgctgccggg
     1441 ggcgacggcg ctgctgatca gcgagcggct tcgaaccgtg atcgccgccg ccgtgacgat
     1501 cgcggtgatc ggcgccgccg gcggcgtcgg gttgaacctg cactggcggt tcatccccgc
     1561 ggggccggcg atcgtgctgg tattgttcgt cgaatttgtc ttgagctacg tagcgagcgt
     1621 ggtggcgcgc cgttgaacgc gccgttcgcg ctccaatagt gatttcttac ggtcaacccc
     1681 ccagcggtac ccagagattt gacccgttcc gcgcacgatt cgatgacatg gcaccaccac
     1741 cgcaacgggg ttggtcgcgc atgcgcgggc gaccgcccgc gctgcggccg gtcgaccaac
     1801 gctcgccgcc agttgctgat aacttcgcgt ctcgcccggc cggatggcgc acagggcggc
     1861 ccacacggtc cgctgaaaga gggtgccgcg catgtccagc ggcaccgcgc cgatggtcgg
     1921 ccgcccatcc agcaaacgca aaacctgcgc cagcgccggg acatccccgg gcgtgatcgt
     1981 cgcggcggga aactcttgat tcagctcgcg caccagtggc cgctcggtgt cgccgagttt
     2041 cacggcgcat acgcccttgg cggttgtcgc gaccagcagc ttcccgagcg agcaatcgac
     2101 gacggcgtaa cgaatgtgta cgccgcgccc accgtcgcgg taacagccgg gcgtcatgcc
     2161 gagctggcgg tcggcgcgct cgtatagccg ggcgggtgac ggaaatcccg ccgccagcgc
     2221 cgcgtgcgtc accgaccgtc cgttgcgcaa gttcgacttg agccgctcga ctcgccgggc
     2281 gcgatcgaac gcgcccggcg tcataccggt cttggcgcga agcaggcggc gcaggtgctg
     2341 aaccccgacg ccattttgct gcgcgaattc attcagcgct cgtgggacat cagtgcgacc
     2401 atcgatgaat ttaatcagcg catcgacggt ggattccgct cccggccggc aacgccggca
     2461 ggcgcggtag cccgcccgtt ccgccatcgc gggcgtctca aaaaactcca cctgttccgg
     2521 gcccggccga cgcgacggac acgacggttt gcagaatacg cccgtcgatc gcaccgcgta
     2581 cacgaaccgg ccatcctgcg tcgcatcgtg cgccagcacc gcttgccagc gatggtcgtt
     2641 tcccgttgct cgtcgtttca tcttcacgcg attgaacata gccacgccgt cgcagcggca
     2701 ctatccgttt gtgcgcgcca atttcgaatt ctgccacgcg ctctggtccc ctctcccggc
     2761 gacctgcggt cagcagtacc gggagaaggg gacgagctac atgccattgc ttggtatact
     2821 gtgccgcatg agatcgattg cattgacggc tctgctttca cttctgctgg catctgcagc
     2881 ctcggcggcg tcggtgtcgt tgacgatcac gccggaatgg aacaatgccg gccggcaaat
     2941 cccggccgac ttcctcggtc tgagctacga gaccaaaatg ctcctgccca cccgcgcggg
     3001 ccagcgcttc ttccggcccg acaacacgcc gctcgtctcc atgttcaaat ccctcggcat
     3061 gcgcaacctg cgcatcggcg ggaactccgt tgatacgcca agcgtcgacg tgccgagcga
     3121 ggcggacatc gacagcctct acgccttcgc cgccgcggcg gacgtgcgcg tgatctatag
     3181 cgtgcggatg aaggcggcgg tcgaaccgac ggacgcggcg cgggtcgcga aatacgtgat
     3241 ggaccactac gccgaccgca ccgcggcgat cacggtcggc aatgagccga acatgtactt
     3301 cgtcgattac gcgccgtatc gaaatgacta cgagcgcttc gccagcgcga tcctcgcggt
     3361 ggcgccgaac gcaatcatca gcggctccag cgcgacgccg ggcaagagcc cgtggacggg
     3421 cgagctggtg cgcgacgtcg gcgggacgcc gatgctaaag ttggtgacgc agcattcgta
     3481 tccgggcggg aacgcaacga aggtgaccaa cgccgcccac gcccggcgga ttttattgtc
     3541 gccagagatc gacgccggct acgaagcgtt ctacgccacc ttcgcccccg cggcgatcga
     3601 aaagcatttg ccgtatcggc tggaagaggc gaacagcttc ttccacggcg gggcgcccgg
     3661 cgtgagcaac tccttcgcct cggcgctgtg ggcgctggac tatctttact ggtgggcctc
     3721 gcacgacgcg gccggcatca acttccacac gaacaacgcg atcgtggctg acgagcagaa
     3781 aatccccggc gggtatgaca cgttctggac ggggccgaac ggattcaagg tgcatccgat
     3841 cggctatgcg gagaaggcgt tcacgctggc cgcgcccggc gggcggctcg tgccggtgaa
     3901 gattgccggc gccaatggcg tgaacgtggt ggcgtacgcg acgctcgcgg cggatgggac
     3961 gcgatacgtc accgtgatca accgcgaaag cggtgaggct ggccgggcga tcgccgcgac
     4021 gaccggcatg ggggatgggg cgtccaacgc ggaatcgctc acgttagctg cgccgaacaa
     4081 tgacgaggcg gcgttaaccg gcgtcacgct cggcggccag atgatcggat cagctggcaa
     4141 ctggaatggc cgatggaccg agtcgacgcc ggacgtgctt gtgatgccga cgagttgcgt
     4201 ggtgatcagg gcgtcgagcg tccagcggcg ttgaaggtga cgaagaggat gcacagatcc
     4261 tgggaccctc tcgaggtcaa gaagtttttc agtggtgacg attcgcagcg cgcctcgtta
     4321 agcttccccc cacattggat cgcgtgagag gaaggacgcg cggcggatga ggagtcaatt
     4381 gaacagcgga gcaaggattt tcgagcttcg ttcaaaattt cacgcgcgct tttgacagcc
     4441 gccgtcacat agctatccta ccgcgccctg acggaacgaa agcccagcag acatggccca
     4501 gatattccat ccaagcgcta acaccatcgc caaggcgagc attttcggag ccgtgtttgt
     4561 cgcggcgttc gtgggttact gcgcggcggg atacgaccgc tcggtgtggc acacgcgggc
     4621 cggttacgcc aaggcccaac cggtgccgtt cagccacgag catcacgtgc gcgggctggg
     4681 gatcgactgc cggtactgtc acacgtcggt ggaaacctca cattttgcgg ggattccttc
     4741 gaccaagacc tgcatgacgt gccacagcca ggtgtggacg ggcgcggcgt tgctcgagcc
     4801 ggttcgcgaa agctggcgga ccaatcgccc gatcgaatgg aaccgcgtgc acaacctgcc
     4861 ggagttcgtt tacttcaatc acagcattca cgtgaacaag ggcatcggtt gcagcagctg
     4921 ccacggtcag gtggacaaga tgcccttgat gtggcaggag ggatcgctgc agatggagtg
     4981 gtgcctgaac tgccaccgcg atccggcgaa gttcgtgcga ccccgcgcgg agatttacaa
     5041 cttgaactat gacccggcga tggacccaag cctgcctgcg aacatgcggt cgcaggagac
     5101 gttgggaccg cagctggtga aggattatca cattcatacg gagcagctga cgaactgctc
     5161 gatctgtcac cggtaggcca gtgatagtta ctgcgataaa cgaagtgtca gtcgaaggtt
     5221 aacgacaaag atcccaaatg ctcgacgcgt gtcctcatca tcacgaagac gaagctgaga
     5281 agctcgacgg gctgaccctg cgcacgccgc ggacaagcca gggcaagcgc atgtggcgca
     5341 gcctggacga gctggcgcag acgccggact tcctgaacat gatgcatcgg gagttcccgc
     5401 atgcggcgtc ggaatggacc gatgaaccga gccggcggaa tttccttaag ctgatgagcg
     5461 cctcgcttgc gctgggcggg ttgacggcct gcacgctgca gaagacgccg gaaaagatcc
     5521 tgccgtacgt caatccaccc gaagaagtga ttccaggccg gccgctgttt tttgccacca
     5581 gcgcgccgtg gtgcggttac gccaagggcg tggtcgtgga aagtcacgaa ggccggccga
     5641 ccaagatcga aggtaacgcg gagcatccct cgaccggcgg gtacggcggg acggacatct
     5701 ggatgcaggc gtcggtgctg gacatgtacg atccagatcg aagccagacg gtgatgcacg
     5761 gctacgggaa cgtcagcacg tggggcgcgt tcacggatga gttgtcgggg aagatggccg
     5821 agcagcagtc caagggcggg gcggggctgc ggtttctgac cggaaccgtg acgtcgccga
     5881 cggtggcccg gcaaattcag ggcatcttga agaagttccc gcaggctaaa tggcatcagt
     5941 gggaagcggt ggggcggcac aatgcgcgcg atggcgcgct gcgggcgttc gggcaggatg
     6001 tgcaggcggt ttatcgcctg gatcaggcga aggtcatcct gtcgctggat tcgaatttcc
     6061 tgctggaaga tccgggcagc ctggcgtacg ccaagcagtt catcgacggc cggcgcgtcc
     6121 gcagggaccg cgacgagatg aaccggctgt acaccgtcga aagcggcgtg acgatcaccg
     6181 gcgcgatggc ggatcatcgg ctggccctgc ggagtagtga gatcgagagt ctcgcgcagc
     6241 aattggccgg cgcgatcaac ggcaccgccg ccagcggccc gaacgcgaag tgggtcaacg
     6301 cggtggctaa ggacctgcag gccaaccgcg ggcacgcgct ggtgctggtg ggtgaaagcc
     6361 aaccggcatc ggtccacgcg ctgggttatg cgatcaatga tgccctgggc gccgcgggca
     6421 gcacggtggt ttacgtcgat ccgatcgaaa cgagcggacc ggatggcccg cacacgctcg
     6481 aggaactggt cagcgacatc aacggcggca ccatcgacct gctcgtgatt ttgggtacga
     6541 acccgtcatt caccgcttac agcgacatgg gttttgtgtc cggcgccggc gagaagacgc
     6601 cggaagaccg cacgcaggcg tacaacgtca agaacccgct gtcgaaggtg aagacggtcg
     6661 tccaccacgg cacgcatttc gacgaaacgg cattcttcag ccactggcac attccagcgt
     6721 cgcactacct ggaaagctgg ggcgatctgc ggtcgtacga cggcaccgcg tcgatcgtgc
     6781 agccgctgat tttgccgctg tattcgagca aatcggacgt cgagctgctg gcgttccttg
     6841 acggtgacgc caaccgcggc gcgtacgaca tcgtgcgcga gacctggcaa agccagatgg
     6901 ccgcgccata cgaggcgacg tgggcgaagt ccctggagaa gggcgtcatc gacggtagcg
     6961 ccgccaagcc caaacaggtg gcgttgaaga cgaacatcgg cgcgttgctc ggcggcgcga
     7021 cgacccagcc ggcgacggcg gggggattgg aactggtctt ccggcccgac ccgcacatct
     7081 gggacgggcg cttcgccaac aacggctggc tgcaggaact gcccaagccg atcacgctgc
     7141 tgacgtggga taacgccgcg ttaatgggct acaacacggc ccggaaactc ggtgtcatcc
     7201 agaacgaccc gggccgggcc accgaagcgt cgatgatcga aatcaaacat ggcagcacga
     7261 cgctgcgcgt gccggcgctg gtggtgccgg ggcacgcgga agatgcgatc acgctgcacc
     7321 tgggatacgg ccgcagccgc gccgggaatg tcggtaatgg caaaggcgtg aacgcatatg
     7381 acatgcggac gcgcgcgacg ccgtgggttg tcgccggcgt tgaggttaac ctcacgggcg
     7441 atatgttccc gctggcgacg tcgcagagcc atcacctgat ggaagtgggc cgggcgccgg
     7501 agatggacaa gtcgtggatg gcagagcgcg agctgttcca cgtcttcacg cgcgacgaat
     7561 ttgccgagaa gctcgaagag tatcacaagg cacagggcgc gaacgacgcg aggaagctgc
     7621 gcacgatcga gctgccgctg ttgaagcaca atcctgacga agaagccgag tcgatgctgc
     7681 ccaactggga ctacagccac aacaaatggg gcatggtgat cgacaacaac gcgtgcatcg
     7741 gctgcaacgc gtgcgtgatc gcctgccaga gcgaaaacaa cattccggtg gtgggcaagg
     7801 agcaggtgct tcgcggccgt gagatgcact ggctgcgcat cgacacctac tggtacggca
     7861 aacccgaaga agcgccggac gtgtacttcc agccgatcac ctgcatgcac tgcgaaaccg
     7921 cgccgtgcac gctggtttgc ccggtggaag cgacgagcat cagtgctgaa gacatcaacg
     7981 agcagacgta caaccgctgc gtcggcacgc gttattgctc gaacaactgc ccgtataaag
     8041 ttcgccggtt caacttcctg tactacaacg attacaagac gccgagcctg atgctgcagc
     8101 gcaacccgaa cgtgacggtg cgcagccgcg gcatcatgga gaagtgttcg tactgcgtac
     8161 agcgcgtgaa caacggtcgc atcgaagcaa aaaaacagga ccgcctgatc gcccccggcg
     8221 aagtgcggac cgcgtgcgag cagtcatgcc cgacgcaggc gatcatgttc ggtaacttga
     8281 acgacccgcg ttgggatgcg accaagctgc aggacgagcc gcaccggtac acgctgttgg
     8341 atgaactgaa taccaagccg cggacgagct atttgtcgcg gttgaagaat ccgaacccgg
     8401 agctagtggg ttcggcgtga agccccttcc ggccccctcg cacacgtact cgtgggagag
     8461 ggctggggtg agggtgattt tgaatctcga gcggtgttgg tgaaagcaaa tcaccctcac
     8521 cctaaccctc tcccggtgta ccgggcgagg ggaccagagt ctggtaagga cgcatggctg
     8581 acaaacccct ccatccccat ctgcacaccg tctccacgat ggacgatgag cgcctggtcg
     8641 ttccgccgcc ggttgttggc gaggggtata cgatcgcgcg cgtcagcgac aagatcgctt
     8701 cggtcgtcct gacgcatccg gtcaccaagg gctggctgct ggggttcacg ctcgccttta
     8761 cgctggtgat ggcgttgacg atggcgatcg cctacctgtt caccaagggc gtgggcatct
     8821 ggggcatcaa catgccggtc gcgtgggggt tcgcgatcgt caactttgtg tggtgggtcg
     8881 gtatcggtca cgcgggcacg ttcatcagcg cgttcttgct gctattgcgg caggattggc
     8941 gaacgtcgat caatcgattc gccgaagcaa tgacgctctt cgccgtggcg tgcgccggca
     9001 tgttcccgct gctgcacgtg gggcgaccgt acatggcgta ctggctgctg ccgtatccga
     9061 acacgatgct gctctggccg cagttccgca gcccgctggt gtgggacgtg ttcgcggtct
     9121 cgacttacgc gacggtgtcg ctgctgttct ggtacgtcgg tctgatcccc gacttggcga
     9181 cgatgcgcga ccgcgcgccg cactggtatg gccggttggc gtatggcgtg ttttcgctcg
     9241 gatggcgcgg gtcggcccgg cattggcatc ggtatcaagc cgcttacatc ctgctcgcag
     9301 gtttggcggc gccgctggtg gtttcggtgc acagcgtcgt gggtatggac ttcgccgccg
     9361 ggcaggtgcc ggggtggcac tcgacgatct tcccgcccta cttcgtcgcc ggtgcgatct
     9421 tctccggttt cgccatggtg gtgacgctcg cgatcccgct gcgctatttc tacggcctgc
     9481 gcgatttcat cacgctgcgc cacttggaca actgcgccaa gctgatgctc gtcaccggcc
     9541 tgatcgtcgc ctacggatac ggcattgagg cgttcatgg
//
LOCUS       DATIMH010000322         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_937469, whole genome shotgun sequence.
ACCESSION   DATIMH010000322 DATIMH010000000
VERSION     DATIMH010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937469"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..>1519)
                     /locus_tag="VLI90_05490"
     CDS             complement(18..>1519)
                     /locus_tag="VLI90_05490"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV13691.1"
                     /translation="FGAAHHMTQLNRRAFLKSTSGLVALAALDLANLHCDVHRQKPAI
                     SRAPKGPNEQLNVAVIGLGGRSSAHIPKFGPNNNCRITHVCDPDTALAQTAIDRARAA
                     NGDRVPDARYVRDLRHIFDDRNVDLVTIATCNHWHSLAAIWAMQAGKDVYVEKPLSHN
                     LSEGRRVVQVAERTGRICQHGSQMRSNPGITEAMAFLHEGHLGNVMVSRGLCYKPRPS
                     IGKVSGPQPIPPTVDYNLWCGPAPMAPLMRMKFHYDWHWNWQTGNGDIGNQGIHEIDL
                     ARWALNTSAMPRSVLSIGGRFGYDDDGQTPNTQIAAYDFGPAQPRLIMEVRGLKTSCF
                     DSNGVENVVACERGFLVFPSYTSAIAYDLDSNIIRVFDGGEDALHFNNFVDAVRSRRP
                     QDLHGPVLEGHRSCALLHMANISHRLGTATPLASRGDDLFNDPDANRAMQRMRQHLVD
                     NQVPLTDKNFVIGRRLSYDDATEMFHNDAEANRMLTRDYRASFIVPASV"
BASE COUNT          242 a          469 c          512 g          296 t
ORIGIN      
        1 caccggcgtc gggggaatca aacacttgcg ggaacgatga acgaagctcg gtagtcgcgc
       61 gtcagcatcc ggttcgcttc tgcgtcgttg tggaacatct cggtcgcgtc gtcgtagctt
      121 aacctgcgcc cgatgacgaa gtttttgtcc gtgagcggga cttggttatc gaccagatgc
      181 tgccgcatgc gctgcatcgc gcggttggcg tcgggatcgt tgaacagatc gtccccgcgg
      241 ctcgccagcg gtgtcgcggt gccgaggcga tggctgatgt tcgccatgtg cagcagcgcg
      301 cacgagcggt ggccctcgag caccgggccg tgcaggtcct gcgggcgccg gctgcggacg
      361 gcatcgacga aattgttgaa gtgcagcgca tcttccccgc cgtcgaagac gcggatgatg
      421 ttcgagtcca ggtcgtaggc gatcgcgctg gtgtatgacg ggaagacgag gaacccgcgt
      481 tcgcacgcga cgacgttctc gacgccgttg ctgtcgaagc aactggtctt cagcccgcgg
      541 acttccatga tcaaccgcgg ctgagcgggg ccgaaatcgt acgcggcgat ctgcgtgttg
      601 ggcgtctgcc cgtcgtcgtc gtagccgaag cgaccgccga tgctgaggac ggatcgcggc
      661 atcgcgctgg tgttcagcgc ccagcgggcc aggtcgattt cgtggatgcc ctggttgccg
      721 atgtcgccgt tgccggtctg ccagttccag tgccagtcgt agtgaaactt cattcgcatc
      781 agcggcgcca tcggcgccgg gccgcaccac aggttgtaat ccaccgtcgg cgggatcggt
      841 tgcggtccgc tgactttgcc gatgctcggc cgcggtttgt agcacagccc gcgcgagacc
      901 atcacgttgc cgagatgccc ttcgtgcagg aacgccatcg cttcggtgat gccggggttg
      961 ctgcgcatct gcgagccgtg ctggcagatc cgcccggtgc gctcggccac ctgcacgacg
     1021 cggcggcctt cgctgaggtt gtggctcagc ggcttttcaa cgtacacgtc cttccccgcc
     1081 tgcatcgccc agatggccgc cagcgaatgc caatgattgc acgtggcgat cgtgacgaga
     1141 tcaacattgc gatcgtcgaa gatgtgacga aggtcgcgga cgtatcgcgc atccggcaca
     1201 cgatcgccgt tggcggcgcg ggcgcgatcg atcgccgtct gcgccagcgc cgtgtccggg
     1261 tcacaaacgt gcgtgatccg gcaattgttg ttcggaccga acttggggat gtgcgccgac
     1321 gagcgcccac cgaggccgat gacggcgacg ttcaattgtt cgttgggtcc cttgggagcg
     1381 cgcgagatcg ccggcttttg acggtgcacg tcgcaatgca gattcgccag gtccagcgcc
     1441 gccagcgcga ccagaccgct cgtcgatttc aaaaacgccc ggcgattgag ttgcgtcatg
     1501 tgatgcgccg ccccgaaag
//
LOCUS       DATIMH010000323         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2669679, whole genome shotgun sequence.
ACCESSION   DATIMH010000323 DATIMH010000000
VERSION     DATIMH010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2669679"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VLI90_05495"
     CDS             <1..301
                     /locus_tag="VLI90_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13692.1"
                     /translation="MEPAKNQELLAQMSQIGQLETSTQLQTSLKSVTLQTQIGSASAL
                     IGKSVQGLDSNGNPIDGLVNSVKVDGDSVDLQLDSGMSMALSNVTSIGPAPVVTK"
     gene            387..2114
                     /locus_tag="VLI90_05500"
     CDS             387..2114
                     /locus_tag="VLI90_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HSV13693.1"
                     /translation="MALTSTLFTGLSGLDVNQTRMNVVGNNIANVNTVAYKASRALFK
                     PQFYVTDAAGSPPSSDFGGTNPSQRGLGASVATIQKDFTQGSIEPTGKPTDMAIDGQG
                     FFVVQDQGNGQMFTRDGSFTLNANHELVTSGGAFVQGYGVDNNGVVLPGQLNNLTIPL
                     GSATIAKATENVQLVGNLAADGQVASGQSITDSQDMQLSPAAAAVAGTTVPATTTLLT
                     DLVSTASGNPTFSSGQTLTLSAKRSGNDIPAQTFDVTPTSTVADLQGFFNSALGIDTS
                     VAGAGAGIPAGTAAGSVKFEVTADSGAANALTLTSGGFADAAGNTPFTFTSNPAGVPA
                     GESTHTSMTVYDSLGVPVNVDMTTVLQSSSNNGTTWKFYARSADNIDPANPGATLLGT
                     GTLSFDTNGKLKTVTGGNLTVHRANTGATPTLPIQLDFTGVSSLAQDAAHKGSDMEMS
                     SQDGIQLGTLVGFSVGADGTITGAFDNGKTRSLGQLAVATFNNPQGLDDKGGNMFAAG
                     ADSGVPIITAPETLGAGGVRSGALEQSNVDLSSEFINLIVASTGFSASSRVISTSDQL
                     MQELLNTQH"
     gene            2352..2546
                     /locus_tag="VLI90_05505"
     CDS             2352..2546
                     /locus_tag="VLI90_05505"
                     /inference="COORDINATES: protein motif:HMM:NF018043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HSV13694.1"
                     /translation="MIALTRLNGQQFVINAEKIRYIESTPDTVVCCDTGEKMMVKETL
                     HEVMKRAIEYARVVRRPITD"
     gene            2683..3894
                     /locus_tag="VLI90_05510"
     CDS             2683..3894
                     /locus_tag="VLI90_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13695.1"
                     /translation="MSRRFIVQPQFALLIMLFSLVPRVHGADVPPMVPRIDTALTNAA
                     KFLLAQQSPDGAWRSHRYGLLKDGPSLTPHVALGVHILSAYEPLAAAAVKDAKAYLDG
                     LLDADDHLKRDVDLIYPVYTAAEACRLAELFEDHHAAAVWLALLRRQQLTEALEWRSS
                     DLEYGGWSYALTPPHRPAAGRQRGAWDWSNLSATVDALETLQALHVPADDPAFAAALL
                     FIRRCQNLADDGSVADVKFDDGGFCFSPAEALRNKAGEAGVDARGHTRFRSYGSATVD
                     GVQALQLCGVRDDDPRLLAGRRWLDEHFLADHNPGDFVAANEDIRDATYFYYCRGFSR
                     LAMSPAQARGIAEALLRRQRDDGSWVNRFTDGREDDPLVATPPAVEALLNCRRILQST
                     APATSTSHRNP"
BASE COUNT          756 a         1253 c         1264 g          678 t
ORIGIN      
        1 gatggagccg gcgaagaacc aggagctgct ggcgcagatg agccagatcg gccagctgga
       61 aacgtccacg cagctgcaga cgtcgctgaa gagcgtcacg ctgcagacgc agatcggatc
      121 ggcctccgcg ctgattggca agagcgtgca gggcctggac tccaacggca atccgatcga
      181 cggcctggtg aactccgtca aggtggatgg tgactccgtc gatctgcagc tcgattcggg
      241 catgagcatg gcgctgagca acgtgacctc gatcggcccg gcaccagtgg taacgaagta
      301 gcaacggtgt aaagccggcg atggcatcgc cggtcttgtt gattttcaaa ccggcgatcc
      361 catcgccggc ttaacaggga caaagcatgg ctctgaccag cacgttgttc acgggactgt
      421 ccggtttgga cgtcaaccag acgcggatga acgtcgtcgg caacaacatc gccaacgtca
      481 acaccgtcgc gtacaaggcc agccgggcgc tgttcaaacc gcagttctac gtgaccgacg
      541 ccgcggggtc accgccgagt tcggacttcg gcggcaccaa tcccagccag cgcggcttag
      601 gcgcgagcgt ggcgacgatc cagaaggatt tcacgcaggg ctcgatcgaa ccgacgggca
      661 agccgaccga catggccatc gacgggcagg gctttttcgt cgtgcaggac caaggcaacg
      721 gccagatgtt cacccgcgat ggttcgttca cgctgaacgc gaaccacgag ctggtcacca
      781 gcggcggggc gttcgtgcaa ggctatggcg tggataacaa cggcgtcgtc ctgccgggcc
      841 agttgaacaa cctgacgatc ccgcttggct ctgctacaat agcaaaagca accgaaaacg
      901 tgcagctggt cggcaacctt gccgccgatg gacaggtggc ttcgggccag tcgatcacgg
      961 atagccagga catgcagctt tctcccgccg ccgcggcggt cgcggggaca accgtgccgg
     1021 cgacgacgac gctgctgacc gatctcgtct caacggccag cggcaatccg acatttagca
     1081 gcggacagac gctgacgctc agcgccaagc gcagcggaaa cgatattccg gctcagacgt
     1141 tcgacgtgac gccgaccagc accgtcgcgg atttgcaggg cttcttcaac agtgcgcttg
     1201 gaatcgacac cagcgttgcc ggcgccgggg cgggaatccc cgcgggcacg gcggcgggca
     1261 gcgtgaagtt cgaagtcacc gctgacagcg gcgctgccaa cgcgctcacg ctaacgtccg
     1321 gcggctttgc cgacgccgcg ggcaacacgc cgttcacctt cacgtcgaac cccgccggtg
     1381 tccccgcggg ggagagcacg catacgtcga tgacggtgta cgattcactg ggcgtgcccg
     1441 tgaacgttga catgacgacc gtgctgcagt catcgagcaa caacggcacg acctggaagt
     1501 tctatgcccg aagcgcggac aacatcgacc ccgcgaatcc gggcgcaacg ctgctcggca
     1561 ccggcacgct gagtttcgac accaacggca agctcaagac cgtcacgggc ggcaacctga
     1621 ccgtccatcg cgccaacacc ggcgcgacgc cgacgctgcc gattcagctg gatttcaccg
     1681 gcgtttcgtc gctggcgcag gacgcggccc acaagggatc ggacatggaa atgtccagcc
     1741 aggacggcat tcagctcggc acgctggtgg gattttccgt cggcgccgac ggcacgatta
     1801 ccggcgcatt cgacaatggc aagacgcgct cgctcggcca gctcgcggtg gccacattta
     1861 acaacccaca aggcctggac gataagggcg gcaacatgtt cgccgccggc gccgacagcg
     1921 gcgtgccgat catcaccgcg cccgaaacgc tcggcgccgg cggcgtccgc tcgggcgcgc
     1981 tggagcagag caacgtcgat ttgtcttcgg aatttatcaa cctgatcgtc gcatcaacgg
     2041 gtttttcggc gtccagccga gtcatctcga cgtcggatca gctgatgcag gagctgctga
     2101 acacgcagca ctgatgattg aacgaatgaa cgccgccaag cgcgctgcca aacaccagcg
     2161 ccggcggcca agatggccgg ggaggatgcc ccggccgatg cgaaactgag ccctcgcggc
     2221 aggtgtttat tctgctgtcg cgggggcctt tttaagacaa cgatgaacgc tgaacgttga
     2281 atgatgagta gcaggctgct cctcgttcat ccttcatcgc gcatcgtttg attgaagatc
     2341 tgggaccgat catgattgca ctcacgcgac tcaatggcca gcagttcgtc atcaacgctg
     2401 aaaagatccg ctacattgaa tccacgcccg acacggtcgt atgctgcgac accggcgaaa
     2461 agatgatggt gaaggaaacg cttcacgaag tgatgaagcg cgccatcgaa tacgctcgcg
     2521 tcgtccgtcg gccgatcacg gattgaagcg cgccgaaacc tgagccggca tcaatcacta
     2581 gccgcgccgc tttacatggg ctgtgacgtt cgcgcgaacg cccacggctc tgccgtggga
     2641 cgccgctcgc gcgcttgcgc cgcttgcggt acaatcgcga ccatgtcgcg gcgctttatc
     2701 gtccaaccac agtttgccct tctcatcatg ctgttctcgc tcgtgccacg agtgcacggt
     2761 gcggatgtgc cgcccatggt cccgcggatc gacaccgcgt tgacgaacgc ggcgaagttc
     2821 ctgctcgctc agcaatcgcc cgacggtgcg tggcgatcgc atcggtatgg actgctgaaa
     2881 gacgggccgt cgctcacgcc gcacgttgcg ctgggcgtgc atattctttc ggcgtacgag
     2941 ccgctagccg cggcggcggt gaaggacgcg aaggcgtatc tggatggttt gctcgacgcg
     3001 gatgatcacc tcaaacgtga cgttgacctg atctatcctg tctacaccgc ggcggaggcg
     3061 tgtcgtctgg ctgaattgtt cgaagatcac cacgccgccg ccgtgtggct cgcgcttctg
     3121 cgtcgccagc agctgaccga ggcgctcgaa tggcggtcga gtgacctgga atatggcggg
     3181 tggagttacg cattgacgcc gccgcatcgg ccggcggcgg ggcggcagcg cggggcgtgg
     3241 gattggtcga acctgtcggc gacggtcgat gcgctggaga cgctgcaggc gctgcacgtg
     3301 ccggcggatg acccggcgtt cgcggcggcg ctgctgttca tccgccgctg ccaaaacctg
     3361 gcggacgacg gttcggtcgc cgacgtcaag ttcgatgacg gcggattttg cttttcaccg
     3421 gcggaggcgc tgcgcaacaa agccggcgag gccggtgtcg atgccagggg ccacacgcgg
     3481 tttcgctcat acggcagcgc aaccgtcgat ggcgttcagg cgctgcagct ctgcggcgta
     3541 cgcgatgatg acccgcgcct cctcgccgga cggcgatggc tcgacgagca ttttcttgcc
     3601 gaccacaacc ccggcgattt cgtcgccgcc aacgaagaca ttcgcgatgc gacgtacttc
     3661 tactattgcc gcgggttcag tcgattggcc atgtcgccgg cgcaggcgcg cggcatcgcc
     3721 gaagcgctgc ttcggcggca gcgcgatgac ggatcatggg tgaatcgctt tacggacggg
     3781 cgcgaagacg atccgctggt ggcaacaccg ccggcagtcg aggcgttgct caactgccgg
     3841 cggattctgc aatcgactgc gcccgccaca tcgacgtcac accgaaatcc gtgatcgtcg
     3901 ctggccagct cgcgcggcct cacttcgccg gcacgtccgg cggcaggtct t
//
LOCUS       DATIMH010000324         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3824735, whole genome shotgun sequence.
ACCESSION   DATIMH010000324 DATIMH010000000
VERSION     DATIMH010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3824735"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VLI90_05515"
     CDS             complement(<1..214)
                     /locus_tag="VLI90_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSV13696.1"
                     /translation="MPQNELLSDVLRRDVGFLQMTVSDMSDGELAQRPVPGANTALWQ
                     LGHLITADAHMVNACAGRTVVELPAGF"
     gene            complement(334..2301)
                     /locus_tag="VLI90_05520"
     CDS             complement(334..2301)
                     /locus_tag="VLI90_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13697.1"
                     /translation="MTASTDASLCGVCGSAIGDAEAACGHCGATPQWRNVDNALRFAR
                     AAFADWRDRNLIDAAQWEARDQHLATALQRVGEQAREGKPVPPDIQLSSPSACWRCAR
                     PVAGFYSYCNTCGAPLGQAADTLRYLAYLARELSRTPTHELTLAQINACTADVQRQMA
                     ALRAELETGRLSPDALTNKRLDHLREMKSTTTTVAVAAAPIASAPAAPAAAEIIALER
                     RSILEILLDPRNIQWLLGSGGGLLVAGLVIWLASLGVFKNALVVAGCMGVGTAALLAG
                     GCSIVKLTRYQLAGRAITLLACLIMPLNLWFYHAHGLVTLDGHLWAAALVCCALYAMA
                     AAVLEDPLFVYVLSAGVAMTGLLILFDLHQLMEAAAPSTLLVVLGLIALHVERACADN
                     DVTFSRKRFGMACFWSAQALLGAGLLWLMGAQLAGTFPIALSWMSGRAILAIDSSQHW
                     LTVGLVLAGAYAYLYSDLVVRRVGVYVYLSAFALLWAQVLVVAVTQLLDHPAVVIAAL
                     SLIAGAVNAAQALALSRRALDSSPRLARALPVVGLILTAAPVLLGVTLDLRANAIALH
                     DVWPYSMNWTLMAAIALVALCCRISALVFNKHHPRLSDEQTNRSAASDDIADKLWTCL
                     LQCAQKRRWSSPGMASQSAGHGLATSSVSSL"
     gene            complement(2477..2830)
                     /locus_tag="VLI90_05525"
     CDS             complement(2477..2830)
                     /locus_tag="VLI90_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSV13698.1"
                     /translation="MTIGDNRELCISLEDRAGALVLRVVGQVGIPSEADQLEGALARA
                     FLHCPRLMVIDLAQMTAISSHGISALLKWRPQIRERIAPLRLAAPQPHVLDLMQRTGM
                     VELFEVFPTVDAALS"
BASE COUNT          582 a          984 c          953 g          477 t
ORIGIN      
        1 cgaatcccgc cggcaactcg acgaccgttc gcccggcgca ggcgttgacc atgtgcgcgt
       61 cggcggtgat caggtgcccc agttgccaaa gcgccgtgtt ggcgccaggc accggccgct
      121 gggccagctc gccgtcgctc atgtcgctga cggtcatctg caaaaagccc acatcccggc
      181 gaagcacatc ggaaagcagt tcgttctgtg gcatgaaatt cctcgctctg aaaaaggtaa
      241 ttgtccgcgt cgcgcgcttg aaaggttaca cgatgtggcg gcgagagaga aacttttccc
      301 gccgatgcgc gtcgataaaa acatgcccgc cacctataga cttgagacgc tcgaagtcgc
      361 caagccatgc cccgccgact gggatgccat gcccggcgat gaccagcggc gcttttgcgc
      421 gcattgcaat aagcatgtcc acaacttgtc ggcaatgtca tcggatgccg ccgagcggtt
      481 ggtctgctca tcggacaggc gcgggtgatg tttgttgaac accagcgcgg agattcggca
      541 gcacagcgct accagcgcga tcgccgccat cagcgtccaa ttcatggaat acggccacac
      601 atcgtgcagc gcgatggcat tcgcccgcag gtccagcgtc acgcccagca gcaccggggc
      661 tgccgtcagg attaacccga ccaccggcag cgctcgcgcc agtcgcgggg aactatcgag
      721 cgcccgccgg cttagcgcca gcgcctgcgc agcattcacc gcccccgcga taagcgacag
      781 tgcggcgatt accaccgccg ggtgatcgag caattgcgtt acggcaacga ccagcacctg
      841 cgcccacagc agcgcaaagg cggagaggta gacgtacacg ccaacgcgac gcacgaccag
      901 gtcagagtag agatacgcat acgccccggc gagcacgagg ccgacggtga gccagtgctg
      961 agatgaatcg atcgcgagga tcgcgcggcc gctcatccac gaaagggcga tcggaaacgt
     1021 cccggccagc tgagcgccca ttaaccacag cagccccgcg cccagcagcg cctgcgccga
     1081 ccagaagcag gccatgccga atcgctttcg cgagaacgtc acgtcgttgt cggcgcacgc
     1141 gcgctcgacg tgcagcgcga tcagcccgag cacgacgagc aacgttgacg gggcggccgc
     1201 ttccatcagc tggtgaagat cgaacaggat gagcagcccg gtcatcgcca cgccggccga
     1261 cagcacgtag acgaacaacg ggtcttccaa caccgcggcc gccatcgcat agagcgcgca
     1321 gcacaccagc gctgccgccc acaaatgccc gtccagcgtg accagcccgt gcgcgtgata
     1381 gaaccatagg ttcagcggca tgatcaggca cgccagcagc gtgatcgccc ggccggccaa
     1441 ttgatatcgc gtcaacttca cgatcgagca cccacccgcc aggagtgccg cggtgccgac
     1501 gcccatgcaa cccgcgacga caagcgcgtt cttgaacacg ccgaggctgg ccagccagat
     1561 gaccagcccc gcgacgagca gtccgccgcc gctgccgagc agccattgga tgttgcgcgg
     1621 gtcgaggagg atttcgagga tcgagcggcg ctcgagggcg atgatctcgg ccgccgcggg
     1681 ggccgcgggc gctgacgcga tcggcgcggc ggcgaccgcg actgtcgtgg tggtggactt
     1741 catttcgcgc agatgatcga gccgcttgtt cgtcagcgcg tctgggctca accgccccgt
     1801 ttccagttcc gctcttagcg cggccatctg ccgctgcacg tccgcggtgc aggcgttgat
     1861 ctgcgccagc gtcagctcgt gcgtcggcgt tcggctcaac tcgcgcgcga gatatgcgag
     1921 ataacgcagc gtgtccgccg cctgaccgag cggggcgccg catgtattgc aataggagta
     1981 gaacccggcg accgggcggg cgcatcgcca gcaggcgctg ggcgatgaca actgaatgtc
     2041 cggtgggacc ggctttccct cgcgcgcctg ctcgccgacg cgctgcagtg ccgtggcgag
     2101 atgctgatcc ctcgcttccc attgggccgc atcgatcagg ttgcgatcac gccaatccgc
     2161 gaacgccgct cgtgcgaatc gcagggcgtt gtcgacattg cgccattgcg gggtcgcccc
     2221 gcagtgcccg caggccgcct ccgcgtcgcc gatcgcgctg ccgcacacgc cacacaaaga
     2281 cgcatccgtt gaagccgtca tggctcctcc cttgtgacgg cccaaacata ctagcgtgtg
     2341 ctaactcggt cgagcgattt gttagtggtc aatagcaaat gatgataagc tgaaatcggg
     2401 acaggaaata gcgacgttcg atctggggaa gaatccgggt cgtcgggagt accctccgaa
     2461 cccggcgtgc ggtgtctcag gacagcgcgg catcgacggt ggggaagacc tcgaacaact
     2521 cgaccatccc ggtgcgctgc atcaggtcaa gcacgtgcgg ttgcggggcg gccaggcgca
     2581 gtggagcaat ccgctcgcgg atctgtggac gccatttcaa caacgcgctg atgccatggc
     2641 tcgagatcgc ggtcatctgc gccaggtcga tcaccatcag ccgcgggcaa tgcaggaatg
     2701 cgcgggcgag cgccccttcc agttggtcgg cttcgctggg gatgccaact tgaccaacga
     2761 cgcgcagcac cagcgcgccg gcgcggtctt ccaaagaaat acaaagctcc ctattatcgc
     2821 caatggtcat ggtgcgcccg acgcacgcca ttgtagatcg aattgcgctt cacgccacgt
     2881 ggggcgaggc gaagcaatat caggcaggtg acggggcttt ggttgccgtg gtcgtggcaa
     2941 tcgcggcggg gacgccgcga atggagaggt tatagacgcc gcccgttttt ccgtcg
//
LOCUS       DATIMH010000325         1588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2092028, whole genome shotgun sequence.
ACCESSION   DATIMH010000325 DATIMH010000000
VERSION     DATIMH010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092028"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VLI90_05530"
     CDS             <1..342
                     /locus_tag="VLI90_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13699.1"
                     /translation="ALGGDPAEQLKQSMHNIDTASERIDGDTATISTGPSETMTLKKS
                     NGTWRVSVAALSPGTTPAAVDERIAVVTAQMKAMQDVTAGILAGKYATATDAVAAIRA
                     RLGGPPPATSP"
     gene            complement(399..878)
                     /locus_tag="VLI90_05535"
     CDS             complement(399..878)
                     /locus_tag="VLI90_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13700.1"
                     /translation="MKSFEVIRQAVDEPGVKAVAGALKVSPALVYKWCEPPAEADDPD
                     QSGAKNPLDRVREMYMLTKDIRLVRWLCNEADGFFVSNPVPDLRKSLDETVFSQTRAM
                     VRDFSELLEAVTESVEDDAAIDPNEADLIRQKWEDLKACVEKFVIGCEKGHFHLRKK"
     gene            1006..>1588
                     /locus_tag="VLI90_05540"
     CDS             1006..>1588
                     /locus_tag="VLI90_05540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HSV13701.1"
                     /translation="MAQTKKQIQSLLSGASTSPRHRFGQNFMIDQNLVRLVAAAGELG
                     PGDVAIEVGPGTGTLTEQLLASPAARVVAVEIDRDLAALLRERLGHDARFQLIEGDAL
                     DGKHAINAELLEVVRASPDRAKLVANLPYNIASPLVIELLLAGVATLAFTVQKEVADR
                     LRAGAGSDAYGPLSVVAQMLSRVEVLRTLPPQAF"
BASE COUNT          281 a          477 c          535 g          295 t
ORIGIN      
        1 gcgctggggg gtgacccggc ggagcagctg aagcagagca tgcacaacat cgacaccgcc
       61 agcgaacgga tcgacggcga caccgcgacg atcagcaccg ggccgagcga gacgatgacg
      121 ctgaagaagt cgaacggcac gtggcgcgtg tcggtggccg cgttgtcacc cggcaccacg
      181 ccggccgcgg tggatgaacg gatcgcggtg gtgacggctc agatgaaggc gatgcaggac
      241 gtgacggccg gcatcctcgc gggcaaatac gcgaccgcaa cggatgccgt cgcggcaatc
      301 cgggcaagac tcggcggccc gccaccggcg acgtcgccgt gaatctgaaa tgaaacttgg
      361 aacgtgaaaa gctcttttca cgtttcacgt ttcgtcggct acttcttccg cagatggaaa
      421 tgccccttct cgcagccgat gacgaacttt tcgacgcacg ccttcaggtc ttcccacttc
      481 tgtcggatga ggtccgcttc gttcggatcg atcgccgcgt cgtcttccac gctttcagtg
      541 accgcttcca gtagctcact gaagtcgcgg accatcgcgc gcgtctggct gaagacggtt
      601 tcgtccaaac tcttgcgcag gtccggcacg gggttggaga cgaagaagcc gtcggcttcg
      661 ttgcacagcc agcgcaccag gcgaatgtcc ttggtcagca tgtacatctc gcgcacgcgg
      721 tcgagcgggt tcttcgcccc gctctgatcc gggtcgtccg cttctgccgg tggctcgcac
      781 cacttgtaga caagggccgg ggaaaccttc agcgcgcccg cgaccgcctt gacccccggt
      841 tcgtcaacgg cctggcgaat gacttcgaaa gatttcatgg gggcgaagtg taacacaaaa
      901 gcgtagcacg ggcgtctcgc ccgtgtcgag gtgttaggca cgggtgggac gcccgtgcta
      961 cgcttcgtgc ccttcgtgcc ttcgtggttt aatgtgttca atcgaatggc acagacgaag
     1021 aaacaaatcc aatcgctgct cagcggcgcc agcacctcgc cgcggcatcg gtttgggcag
     1081 aatttcatga tcgaccagaa cctggtgcgg ctagtcgccg ccgcggggga gctggggccg
     1141 ggggatgtgg cgatcgaagt cgggccgggc accgggacgt tgaccgaaca gttgctggca
     1201 tcgccggcgg cgcgcgtcgt ggcggtggaa atcgatcgtg atttggcggc acttctccgc
     1261 gaacgtctgg gccacgatgc gcgatttcag ttgatcgaag gcgatgcgct cgatgggaaa
     1321 catgcgatca acgcggagct gctcgaagtc gttcgcgcgt cgcccgatcg cgcgaagctc
     1381 gtcgcgaacc tgccatacaa tatcgcctcg ccgctggtga tcgaactgct gctggccggc
     1441 gtggcgacgc tggcgttcac ggtgcagaag gaagtggccg accggctgcg cgccggtgcg
     1501 gggtcggatg cgtatgggcc attgtcggtg gtggcgcaga tgttgtcgcg ggtcgaggtg
     1561 ctgcgcacgc tgccgccgca ggcgtttt
//
LOCUS       DATIMH010000326         5622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_73298, whole genome shotgun sequence.
ACCESSION   DATIMH010000326 DATIMH010000000
VERSION     DATIMH010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73298"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VLI90_05545"
     CDS             <1..899
                     /locus_tag="VLI90_05545"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSV13702.1"
                     /translation="PVDAGVAMVGLDAWVYCNPYHEAAGGGDVYYVSACATGRITRLL
                     VADVSGHGEQVNAVGATLRTMMRRYVNYLDQRRMVRSLNREFTTLTAAGCFATAIVCT
                     FFGPTRHLTLCNAGHPPPLLYRAKERQWTALERGGCDANIPWGIDEVCNYDQFDVRLR
                     VGDLVLCHTDSLSEARKPDGELLGMPGLLRIASTLDVSEPSKLTPALLRAIEAEGYRT
                     DDDVTVLLFRPNGAQPRLSFVERWRVQGRMAKAFARSLIPGGGPMPWPDLRLANIGGV
                     FFSSLNKTWGPRMMRREMAERD"
     gene            complement(1247..1573)
                     /locus_tag="VLI90_05550"
     CDS             complement(1247..1573)
                     /locus_tag="VLI90_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HSV13703.1"
                     /translation="MADQKNYVRTDADGVMRVGARGVPIDGVIAAFEIGDSPEAIRSQ
                     YPALELEEVYGAIAYYLANRQEIAEYLKRQDEVWAYWRSRFEKNPDPLIDRLKAIRAA
                     QSMQAS"
     gene            complement(1604..4228)
                     /locus_tag="VLI90_05555"
     CDS             complement(1604..4228)
                     /locus_tag="VLI90_05555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV13704.1"
                     /translation="MARPGKSKRTKKTTKKQPIKPAIAAPPKPTIKKTVKKTAKKATP
                     STPRPRAATPSPPTVNAKPKTNAKLGRAVSKSAVVAAADELLVAPVRVMPAGDPQPEL
                     ILKPEASPPQPSRAIRSESNNRNAPPPMSFYRPAASNRPASGAPSFAQSPPPQPAPAA
                     PVAEVIAPQNRNTSVSTIERPSEPESTAMRSTTAATPSPAPVSSDVPKLAEAPPAQAR
                     PPVIPSPPPEDRRPHAPAAPAPAPAQPPPAPVREPEPVEPPKTEPEPAKTPAEPILFE
                     IAWEVCWQLGGIYTVLKSKANAMLQRWGDRYCLIGPYNPQTAAYEFDEHAPTGVFRDA
                     IEALAHAGIPAKFGRWLIPGRPQVILIDYRARYNALDSDKYLLWHDHHISAPANDGEV
                     NEVVAFGFATAEFFARLASAVGDRPILAHFHEWMAGVAIPRIAHMRLPIATIFTTHAT
                     LLGRYIAGDSPEFYNHLPYINADEQAAKYQIYPRFAIERAAAHASTVFTTVSEVTATE
                     AEKLLGRKPDAIVPNGLNIQRFAALHEFQNLHQVYKERIHEFVMGHFFPSYTFDLDRT
                     IYFVTSGRYEYRNKGFDLFIEAMYRLNQRLKAMPDRPTVVAFIVTRAWTRHINVGVLQ
                     RESMFEDLKHVCSGLERQMGGRLFRAAANGRLPQMKDLLNDDELLRLRQSIQAWRTGP
                     LPAIVTHDLVDDATDPVLQHLRHRQLFNSADDPVKMIFHPEFVTATSPLFKLDYQQFV
                     RGCHMGIFPSYYEPWGYTPMECVALGLPAVTTDLSGFGAYVEHHVPNAAHQGICVLNR
                     RTKSFEETTNNLVDYVMNFVQLSRRQRIEMRNRVERLSELFDWSALVIHYHEAHDMAL
                     QRLTAGRASTGRLEVRMV"
     gene            4393..>5622
                     /locus_tag="VLI90_05560"
     CDS             4393..>5622
                     /locus_tag="VLI90_05560"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HSV13705.1"
                     /translation="MALFTVETYRELKPHDEQEWLLTNGLGGFASSTIVGMNTRRYHG
                     LLCAATLPPVGRVMTINRIGEILTLDGKSDQLLEFSCNAFRGSVHPRGDQYLDRFELD
                     DTARWHYDVEGVHVTKQLQMLWLKNVVAVRYTVDAGGTRPFELRLLPFASLRDFHATR
                     HAGSASFRVEAGDRRVTVGEGPHEVTITSDDGSFTQQGDWWYGHVYPIETERGQDDVE
                     DLFSPGRFVLSGQGKGTITLWIGLEPTPPGDWEAERKRRCDAVAAACTEPENIGAGRE
                     HGMVTPAMGGRLHPVAASKSAVVQKLFRASNDFVVYRKSPSGEDGTSVIAGYPWFSDW
                     GRDTMISLPGLLLVPGRYRQAREVLTVFAQYVSEGMIPNRFDDYTNEPSYNTVDASLW
                     FVHAVHEYARLSHDSRTF"
BASE COUNT          988 a         1680 c         1878 g         1076 t
ORIGIN      
        1 agcccgtgga cgcgggcgtc gccatggttg gcctagacgc gtgggtctac tgcaatccct
       61 atcatgaggc cgcgggcggc ggagatgtgt actacgtctc ggcgtgcgcg acgggccgaa
      121 tcacgcgctt gctcgtggcg gatgtcagcg ggcacggtga gcaggtcaac gccgtcggcg
      181 ccacgctccg cacgatgatg cggcggtacg tgaactacct ggatcagcgg cggatggttc
      241 gttcgctcaa tcgcgaattc accacgctta ccgccgccgg ttgcttcgcc accgccatcg
      301 tctgcacgtt cttcggcccc acgcggcatc tcacgctgtg caacgccggt cacccgccgc
      361 cgctgctgta tcgcgccaag gaacgccaat ggaccgcgct cgaacgcggc gggtgcgacg
      421 cgaacatccc ctggggtatc gatgaagttt gcaattacga tcagttcgac gtgcgcctgc
      481 gcgtgggcga cttggtgctg tgccacacgg attcattgag cgaagcgcga aagcccgatg
      541 gcgagctgct tggtatgccg ggcttgctgc gcatcgcgtc aacattggac gtctcagaac
      601 catcaaagtt gacgccggcg ctgctgcgcg cgatcgaagc cgaaggctat cgcacggatg
      661 atgacgtgac ggtgctgcta ttccgcccaa acggagcgca gccgcggctg tcgttcgtcg
      721 aacgctggcg cgtgcaaggc cgcatggcca aagcattcgc caggtccctg atccccggcg
      781 gcggcccgat gccgtggccg gacctgcgcc tggccaacat cggcggggtg ttcttctcat
      841 cgctgaataa aacctggggc ccgcgcatga tgcggcggga gatggcggag cgagattagc
      901 cgttgccgcg aatgctctgc acatacagtc acagcggaag aaatgtgatt cgatcccgcc
      961 actcctcagc tgtgcttgca gaccaaatta aaacaacaga ggcgatcacg gccgcgacat
     1021 ccagcgaccg ctggtgcgca atcatcaggc ctggaaattg ttgccgactc tgcagccgcc
     1081 agcacgatca ggtcccgatc cgctgcccat gctagaacgt cagcatcact ggcggcagaa
     1141 agaccaacat cgcgcaatag gattaactga aggttcggct ggagcctgga cacaccgtct
     1201 atgatatctt cgttgaagtc gttatcgacg aggaatgccg accgcgtcac gacgcctgca
     1261 tggattgtgc agcgcgaatt gccttcagcc ggtcgataag cgggtcgggg ttcttctcaa
     1321 atcgcgatcg ccagtaagcc cacacttcgt cctgtcgctt caggtactct gcaatttcct
     1381 gtcgatttgc cagataataa gcgatcgcgc cgtagacttc ttcgagttcg agtgccggat
     1441 actgactgcg aatggcttcc ggtgagtcgc cgatctcaaa cgcggcgatg acgccgtcga
     1501 tcggaacgcc gcgtgcgccg acgcgcatca cgccatccgc atccgttcga acataattct
     1561 tctggtccgc catgatctgc cattatatcg cggataaaag ccatcacacc atccgaactt
     1621 ccaaccgccc ggtgctggct cgaccggcgg tcaggcgttg cagggccatg tcgtgggctt
     1681 cgtgatagtg gatcaccagc gcgctccagt cgaacaactc gctgaggcgc tccacgcggt
     1741 tgcgcatctc gatgcgctgc cggcggctta gctgcacgaa gttcatgacg taatccacca
     1801 gattgttcgt cgtctcctca aaactcttcg tgcggcggtt cagcacgcag atgccttggt
     1861 gggccgcgtt gggaacgtga tgttcgacgt acgcgccgaa gccgctcagg tccgtcgtca
     1921 ccgcgggcag gcccagggcg acgcattcca taggcgtgta gccccacggt tcgtaatagc
     1981 tggggaagat gcccatgtga cagccgcgaa cgaactgctg gtagtccagc ttgaacagcg
     2041 gactggtcgc ggtgacgaac tcgggatgaa aaatcatctt caccggatcg tcggcgctgt
     2101 tgaacagctg gcggtggcgc aggtgttgga gcaccggatc ggtcgcgtcg tccaccagat
     2161 cgtgcgtgac gatggccggc agcggacccg tgcgccacgc ttggatcgat tgtcgcagac
     2221 gcaacaactc atcgtcgttc agcaaatcct tcatctgcgg cagccgccca ttggcggcgg
     2281 cgcggaacag ccggccgccc atctggcgtt ccaaaccaga gcagacgtgc ttgaggtctt
     2341 cgaacatcga ctcgcgctgc agcacgccga cgttgatgtg ccgcgtccac gcgcgggtga
     2401 cgatgaacgc gacgacggtc ggccggtccg gcatcgcctt gagccgctgg ttcaggcggt
     2461 acatcgcttc gatgaacagg tcgaagccct tattgcgata ttcgtatcgc ccgctggtga
     2521 cgaagtagat cgtgcggtcc aggtcgaagg tgtagctggg gaagaagtgg cccatgacga
     2581 attcatggat gcgctccttg tatacctggt gcaggttctg gaactcgtgc aacgcggcga
     2641 agcgctggat gttcagcccg ttgggcacga tcgcgtcggg cttgcggccg agcaatttct
     2701 cggcttcggt ggctgttact tcactgaccg tagtaaacac agtcgaagcg tgggcggcgg
     2761 cgcgttcgat cgcgaagcgc ggatagatct gatatttcgc cgcctgctcg tcggcgttga
     2821 tgtacggcag gtggttgtaa aactccggcg aatcgcccgc gatgtatcgc cccagcagcg
     2881 tggcgtgcgt ggtgaagatg gtggcgatcg gcaagcgcat gtgcgcgatg cgcgggatcg
     2941 cgacgccggc catccactcg tggaagtggg cgaggatcgg gcggtcgccg acggcgctgg
     3001 ccaggcgcgc gaagaattcc gcggtggcga aaccgaacgc gacgacttcg ttgacctcgc
     3061 cgtcgttcgc cggcgcggaa atatggtggt cgtgccagag caggtacttg tcgctgtcga
     3121 gcgcgttata gcgggcgcgg taatcgatca ggatgacctg cggccgaccg ggaattagcc
     3181 agcgaccaaa cttcgccggg atgcccgcat gggcgagggc ttcgatcgca tcccgaaaca
     3241 cgccggtcgg cgcgtgctca tcgaattcat acgccgcggt ctgcgggttg tacgggccga
     3301 tcagacaata gcgatcgccc cagcgctgca gcatcgcgtt ggctttggac ttcagcaccg
     3361 tgtagatccc gccgagttgc cagcagactt cccaggcgat ctcgaacaag atcggctccg
     3421 cgggcgtttt cgccggctcg ggttcggtct tcggtggttc gaccggctcg ggttcgcgca
     3481 ccggcgccgg tggaggttga gccggcgcgg gcgccggcgc agcaggggcg tgcgggcggc
     3541 gatcttccgg tgggggcgat ggaatcaccg gcggccgcgc ttgcgcgggc ggcgcctcgg
     3601 ccagcttggg tacatccgat gaaacaggcg cgggacttgg cgtggcggcg gtggtggaac
     3661 gcatggcggt ggactcgggt tctgacggtc tctcgatggt tgagacggac gtgttccgat
     3721 tctgcggcgc aataacctcc gccaccggcg cggcgggcgc aggttgcggc ggcggagact
     3781 gcgcgaacga cggcgcgccc gatgccggtc gattcgacgc cgccggtcga tagaacgaca
     3841 tcggcggcgg ggcgttgcgg ttgttcgatt cggaccggat cgcgcgcgaa ggctgcggcg
     3901 gcgaggcttc cggcttgagg atcaactccg gttgcggatc gcccgccggc atcacccgca
     3961 ccggcgcgac caacaattca tcggcggcgg cgacgaccgc ggacttagac acagctcgcc
     4021 ccagcttggc gttggtcttc ggtttcgcat tgacggtcgg tggcgatggc gtcgccgcgc
     4081 gcggccgcgg tgtgctcggc gtcgctttct tcgcggtctt cttcacggtt ttcttgatcg
     4141 tcggtttcgg tggcgcggcg atggcgggtt tgatgggctg tttcttcgtc gttttcttcg
     4201 ttcgcttgga tttgccgggc cgcgccatgg cggcattatg acgagagtct gtcggcgggc
     4261 aaatggcgat tgttccagat gaaaccggcg tcgcccgata ggcatctgaa gaggcgcatc
     4321 gtggatgttt tgtcgcgcac cgccaggcga cgcgcatgac tcggtctgta ttccccatta
     4381 cactcggacg ccatggcatt gttcaccgta gaaacatatc gcgaactgaa gccccacgac
     4441 gagcaggagt ggttgctgac gaacgggctc ggcgggttcg cttcgtccac gatcgtcggc
     4501 atgaacactc ggcgatatca tgggctgctc tgcgcggcca cgctcccgcc ggtgggacgc
     4561 gtgatgacca tcaaccgcat cggggaaatt ctcacgctcg acggcaagtc cgaccagctg
     4621 ctggaattct cctgcaacgc cttccgcggc tcggtccatc cgcgcggcga tcaatacctc
     4681 gaccgcttcg aactcgatga caccgcccgc tggcattacg acgtcgaagg cgtgcacgtc
     4741 accaaacaac tgcagatgct ctggttgaag aacgtggtcg cggtgcgata cacggtggat
     4801 gccgggggaa cgcggccgtt cgaattgcgg ctgctgccgt tcgcgagcct gcgcgatttt
     4861 catgcgacgc gccatgcggg atccgcgtcg ttccgcgtcg aagcgggcga tcgccgggtg
     4921 acggtgggcg aaggcccgca cgaggtgacg atcaccagcg acgacggctc gttcacgcag
     4981 cagggcgatt ggtggtacgg gcacgtgtac cccatcgaga cggagcgcgg gcaggatgac
     5041 gttgaagacc tgttctcgcc ggggcgcttc gtgttgagtg gccaaggcaa gggaacgatc
     5101 acgctgtgga tcggattgga acccacgccg ccgggggact gggaggcgga gcggaaacgc
     5161 aggtgtgacg cggtggcggc ggcttgcacg gaaccggaaa acatcggcgc cggccgcgag
     5221 cacggcatgg tcacgccggc gatgggcggg cgattgcatc cggtggcggc gtcgaaatct
     5281 gcggtcgttc aaaaattgtt tcgtgcgtcg aacgattttg tcgtctaccg caaatcgccg
     5341 agcggtgaag acggcacgag cgtgatcgcc gggtatccct ggttcagcga ctggggccgc
     5401 gacacaatga tttcgctgcc gggcttgttg ctggtaccgg ggcggtatcg gcaggcgcgc
     5461 gaggtgctga cggtcttcgc ccagtacgtt agcgaaggga tgatcccaaa ccgcttcgac
     5521 gattacacga acgagccgag ctacaacacg gtcgatgcca gcctgtggtt tgtgcacgcg
     5581 gtgcacgagt acgcccggct gtcgcacgat tcgcggacgt tt
//
LOCUS       DATIMH010000327         3981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5559481, whole genome shotgun sequence.
ACCESSION   DATIMH010000327 DATIMH010000000
VERSION     DATIMH010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5559481"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..855
                     /locus_tag="VLI90_05565"
     CDS             181..855
                     /locus_tag="VLI90_05565"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSV13706.1"
                     /translation="MTLIHTAIDLFLHLDKHLNDFASAHPTGVYAILAAIVFCETGFV
                     VTPFLPGDSLLFAVGALAASTGSPINLPLVIVMLCLCANAGDLINYSIGRRVGPAIFS
                     KEGGSILLSRKHLAEAHAFYERHGRKTIILARFVPIIRTFAPFVAGIGAMPFARFIGF
                     SIGGGILWVVSVSLAGYFFGQIEIVKKHFELVVLAIIGISVVPVIVQALRSRGKTQSF
                     EVVART"
     gene            complement(1081..1998)
                     /gene="atpG"
                     /locus_tag="VLI90_05570"
     CDS             complement(1081..1998)
                     /gene="atpG"
                     /locus_tag="VLI90_05570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HSV13707.1"
                     /translation="MAKARAIVKRRKAVRNIRKITKTMQMIATAKFQKALKRAVGTKP
                     YTRKVRELVAELAASLGGDVQHPLLRNPSDAERTNRIALVVITSDRGLAGGYNGNVLR
                     AANVFFREQTANGKTIHLYVSGKKGTSYFKFQRRPITKRFDQFADMPKFVDVNRIADD
                     FIQQFTSGQLDAVYVAYMNFVSAGTQRPDVAQLLPLASLSTTAATKSADHAGPGKLRS
                     ETIYDFSPDPKTLLDELLPLTVKTALFQAFLDATTSENVARMVAMKSATDNADKIVKA
                     LTMEYNRARQSQITTELSEIMGGVEAMKG"
     gene            2133..2666
                     /locus_tag="VLI90_05575"
     CDS             2133..2666
                     /locus_tag="VLI90_05575"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HSV13708.1"
                     /translation="MFPNGNRLVTFFVGLLLLGLGICGLIHPLLQSPHPEQAEAQHMM
                     VSVNYGFLFGVFAMNIVSDIVFIVLGVAGIVAAYTASISRVYERSLCILSVVFMLMGF
                     MPGEIDKVWGLMPLFGWTDALFFFIALLTFYFGFVEGPPSTFLNQPLEPLSQIEPPND
                     PLGPKHSTRPPTGLSHS"
     gene            complement(2676..>3981)
                     /locus_tag="VLI90_05580"
     CDS             complement(2676..>3981)
                     /locus_tag="VLI90_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSV13709.1"
                     /translation="GWRGASPDAIPRFIENGKRKTGRCAVVKLGTNYRSTPEVIAVAD
                     RLIRCSAERAIAVEFRPVHKSGEPVKCAKLGTPEEEGEAVALSIWNTMKSGVQPREMA
                     VFYRTNDMSRLVEQALAKRQIAYIVVGSGSYYDRMEVKDVLSMLRFLCNPRDGISFAR
                     IANKPARGMGDALIGRLESHAERHGMDLLSALARSEEIRDENDKPLSDAARRACDEAL
                     GVFCFDLSGKSLADVAMEILSRTNYDAWLKDRYEEKGEYESRQRNVNELVNSIAEFSR
                     TSPKASIADYLQSISLYTDGDRSRDDNAVRLMSLHASKGLEFDVVYMIGVEHGILPHE
                     KAVIDRGDRGLEEERRLCYVGFTRARKLLRVTWCQARQDVFNRSKTARYQPSVPSRFL
                     LESGLMTKDEYDAAVNEARFGIKRPTAAASAAAATAAAAKRG"
BASE COUNT          657 a         1330 c         1140 g          854 t
ORIGIN      
        1 cagctctagc ggcaaaagca tcgctcagct cctaactctg cggcctccgc gtctctgcgt
       61 tcatcctcgt tcttcgcacg ctcttgcctg gcatcccgtc gcaagcgcta aatcatgggc
      121 tggtagcgga tgccacgatc ggttgtgcgg gctgcatctg tcgggtatga ccctcgccga
      181 atgacgttga ttcataccgc catcgatctg ttcctgcacc tggacaagca cctgaacgac
      241 ttcgcgtcgg ctcatccgac gggagtctac gcaattttgg ccgccatcgt cttttgcgaa
      301 accggcttcg tcgtcacgcc attcctgccg ggcgattcgc tgctgttcgc ggtgggggcg
      361 ctggcggcat cgaccggctc gccgatcaac ctgccgctgg tgatcgtcat gctatgcctc
      421 tgcgccaacg ccggtgacct gatcaactac tcgatcggcc ggcgcgttgg cccggcgatc
      481 ttcagcaaag aaggcgggtc gatcctgctg agccgcaagc acctggccga agcccatgcc
      541 ttctacgaac ggcacggccg caagacaatc atcctcgccc gattcgtgcc gatcatacgc
      601 acgttcgccc cgttcgtcgc cggcatcggc gcgatgccgt ttgcgaggtt catcggcttc
      661 agcatcggcg gggggatcct atgggtcgtc tcggtgtctc tggcgggcta cttcttcggg
      721 cagatcgaga tcgtgaagaa gcatttcgag ctggtggtgc tggcgatcat cgggatatcg
      781 gtggtgccgg tgatcgtgca ggcgctaaga tcgcggggga agacgcagag ttttgaggta
      841 gtggcgcgaa cgtaattctg gcgccgccag gttgaccacg tcctgcagcg gtttcagaac
      901 cttcaccgtg acacgggcct ccggctgcct ccggcccgtg cgcgctcgct ggttgaaacc
      961 cacgcattct acaattcagt gattcacgca cgggccggag gcacgtgtca ccgaggcgtc
     1021 ttcaattctg aaagcgcctt tttgcggccg ctacatgaaa aagaccaagc gcgctgacaa
     1081 tcaccccttc atcgcttcga cgccgcccat gatctctgaa agctccgtcg tgatctggct
     1141 ctggcgggcg cggttgtatt ccatcgtcag cgccttgacg atcttgtcgg cgttgtcggt
     1201 ggcgctcttc atcgccacca tgcgggcgac gttttcgctg gtcgtcgcgt cgaggaacgc
     1261 ctggaacaac gccgtcttta ccgtcagcgg gagcaattcg tccagcagcg tcttggggtc
     1321 tggcgaaaaa tcatagatcg tctcggacct caacttgccc ggcccggcat gatcggccga
     1381 cttcgtcgcg gccgttgtgc tgagcgacgc cagcggcaac agctgcgcga cgtccggtcg
     1441 ctgcgtgccg gcggacacga agttcatgta cgcgacatac accgcgtcca actggcccga
     1501 cgtgaactgc tgaatgaagt cgtcggcgat acgattcaca tctacaaact tcggcatgtc
     1561 ggcgaattga tcgaatcgct tggtgatcgg ccggcgctga aacttgaagt agctggtgcc
     1621 ctttttgccg gagacataca gatggatcgt cttcccgttg gcggtctgct cgcggaagaa
     1681 cacgttggcg gcgcgaagga cgttgccgtt gtagccccca gccagcccgc gatcgctggt
     1741 gatgacgacg agcgcgatgc gattggtgcg ctcggcatcg gatggattgc gcaacagcgg
     1801 atgctgcacg tcaccgccga ggctggccgc cagctccgcc accagctcgc gcaccttgcg
     1861 cgtgtagggc ttggtgccga cggcgcgctt gagcgctttc tggaatttcg ccgtcgcgat
     1921 catctgcatc gtcttggtga tcttgcgaat attgcgaacc gccttgcggc gtttcacgat
     1981 cgcgcgtgcc tttgccatct cgagtcgtct ccgatgttaa acagcgagcg ggattatggt
     2041 cagcgccggc acatctgcaa gttattgatc gctggcgacg ccgcatcggt acgtcgcttg
     2101 cagcaaatct tattgctgca acggaggtta agatgtttcc aaacggcaat cgcctcgtca
     2161 cgttcttcgt ggggctgctt ctgctcggac tcggcatttg cggcctcatc catccgctgc
     2221 tgcagagccc gcaccccgaa caggccgagg cgcagcacat gatggtcagc gttaactacg
     2281 gctttctgtt cggcgtcttc gccatgaaca tcgtcagcga catcgtcttc atcgtgctgg
     2341 gcgtcgccgg catcgtcgcg gcatacaccg cttcaatctc tcgggtttac gagcgttcgc
     2401 tctgcatact gagcgtggtg ttcatgctca tgggatttat gcccggcgag atcgacaagg
     2461 tctgggggct gatgcctctg ttcggttgga ccgatgccct gttcttcttc atcgcgctcc
     2521 tgacgttcta cttcggattc gtcgagggcc cgccgtcaac cttcctcaac cagcccctgg
     2581 aaccgctgag ccaaattgaa ccaccgaatg acccgttggg cccgaagcat tcgacgcggc
     2641 cgccgacggg gctttcgcat tcgtgaatgg cgcgatcagc cgcgcttggc cgccgccgct
     2701 gtggctgccg ccgcggacgc tgccgccgtg ggacgtttga tcccgaaccg cgcctcgttt
     2761 accgccgcgt cgtactcgtc cttggtcatc aggcccgatt cgagcaggaa ccggctgggt
     2821 acgctcggct ggtaccgcgc cgttttgctg cgattgaaga catcctgccg cgcctgacac
     2881 cacgtgacgc gaagcagttt ccgcgcgcgg gtgaacccca catagcacag ccggcgttct
     2941 tcttcgagcc cgcggtcgcc acggtcgatg acggcctttt cgtgcggcag gatgccgtgc
     3001 tccacgccga tcatgtacac gacgtcgaac tccaacccct tggatgcgtg cagcgacatg
     3061 agccgaactg cattgtcatc gcgcgagcgg tcgccatcgg tgtacagcga gatgctctgc
     3121 agatagtccg cgatgctcgc cttcggtgac gtgcggctga actcggcgat gctgttcacc
     3181 agctcgttca cattccgctg gcgactttcg tactcgccct tttcttcgta cctgtctttc
     3241 agccacgcgt cgtagttcgt tcggctgagg atctccatcg cgacgtcggc caagctcttg
     3301 cccgacagat cgaagcagaa cacgcccagc gcctcatcgc acgcgcgacg cgctgcatca
     3361 ctcagcggct tgtcgttctc atcacgaatc tcctccgacc gcgccagcgc cgacagcagg
     3421 tccatcccgt gccgctcggc gtgcgattcc aatcggccga tcagcgcatc acccatcccg
     3481 cgcgccggct tgttggcgat ccgcgcgaag ctgatgccgt cgcgcgggtt gcacaagaac
     3541 cgcagcatcg acagcacgtc cttcacttcc atgcggtcgt agtaactgcc gctgccgacg
     3601 acgatgtacg caatctgccg cttcgccagc gcctgctcga ccagtcggct catgtcgttg
     3661 gtgcgataaa acaccgccat ctcccgcggc tgcaccccgc tcttcatcgt gttccagatc
     3721 gacaacgcca ccgcctcccc ctcctcctcc ggcgtcccca acttcgcaca cttcaccggc
     3781 tccccgctct tgtgcaccgg ccgaaactcc accgcgatcg cccgctccgc cgaacaccgg
     3841 atcagccgat ccgccaccgc aatcacctcc ggcgtcgacc ggtaattcgt ccccagcttc
     3901 accaccgcgc accgccccgt ctttctcttc ccattctcaa taaaccgcgg gatcgcgtcc
     3961 ggcgatgccc cccgccaccc g
//
LOCUS       DATIMH010000328         5621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2381038, whole genome shotgun sequence.
ACCESSION   DATIMH010000328 DATIMH010000000
VERSION     DATIMH010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381038"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VLI90_05585"
     CDS             <1..492
                     /locus_tag="VLI90_05585"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV13710.1"
                     /translation="LAVAAKPAYVEGLYLQHRNVEALQAADRYLAPGNDLRSEQRSLV
                     SYYRGMILLDLGRVKDAIAVLKAATEQTRTRKIAIINLFEAQLRDDDCVAAEATLNDI
                     TRNYHNTLGRLARLKAELAHAKETHGILPLNEAPRVSNRLSDGSERREPVIGVTEDRH
                     ATR"
     gene            complement(1330..4332)
                     /locus_tag="VLI90_05590"
     CDS             complement(1330..4332)
                     /locus_tag="VLI90_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13711.1"
                     /translation="MDRALFRRPTAEYRGTPFWSWNNRLCIPQLLRQIDQLKAMGFGG
                     FHMHPRTGMETEYLGREFFDAVIACTEHAKRRAMLAWLYDEDRWPSGFAGGLVTKDKS
                     LRAKRLLWTSKEYRGDGVLLARYAVALKDGRLAKYRRLSAAARGRGGWAAYLETTPED
                     SWFNGQTYVDTFSRRAIERFIEITHERYAAAVGQHFGTLVPAIFTDEPQFVKKQTFQL
                     ASDQRDVLMPFTDDFVETYRRAYGQDLLDHLPELFWNLPDDEPSLARYRFHDHTAERF
                     AAAFGDTLGQWCGRHHIALTGHMLGEANLFSQTRGVGEVMRALRGFHLPGIDILQDKM
                     ELTTAKQAQSIAHQFGRPGVLSELYGATNWDFDFVGHKAQGDWQAAMGVTVRVPHLAW
                     VSMAGEAKRDYPASIGPQSPWYAEYPLIEDHFGRVNTVLTRGRPHVRVGVIHPIESYW
                     FSFGSMEQNQPEMQDREEAFRELPSWLLFGLIDFDYISESLLPTQAGGSERDAKRFMV
                     GEMAYDAVLVPSLRTIRATTLQRLERFANAGGAVIFAGEIPSLVDGNRSPAAQKLAAR
                     CTQVGWRRRDILAALSPFRDVEIYHGDGVRADSILHQRRDDGKHVYLFFCNTDREFGR
                     GHARIRVRGKWDVDQLDSMTGRTRPLYSTIDVGDTVIPWSFTPHGHLLIRLSRRTGRS
                     VRAVAEPKLVEIGRIEGPVPVTLSEPNVLMLDQAEWRLNDGEWNAAEEVLRIDDRVRQ
                     ALNLPLRGGRMAQPWVDQSEAPVVALLQLRFSIRSEVTVDQPSLAIEAPETVKIHFDA
                     DEVSNRPTGWWIDESIATVALPALAADVHELLLTIPFTRKTNLEWCYLLGDFGVAIAG
                     RSARIIAPVRELAFGDWTRQGLPFYAGNVTYRCVIDGDDRDLTLAVPKFRAPLLSVNV
                     DDKPAGKIAFAPFELTIGRSRQRRSLEITAFGNRVNTFGAVHNANEKLNWFGPAAWRQ
                     TGASWACEYQLRRMGVLAAPILRVVRRT"
     gene            4881..>5621
                     /locus_tag="VLI90_05595"
     CDS             4881..>5621
                     /locus_tag="VLI90_05595"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13712.1"
                     /translation="MLILDYAHDPHATSPAINADMLKLRQDIGGKSKSLLADQKVDLS
                     HLFILIEGFRLKRDVAFAKDGSRTLAMDVQYPSQPTHPVPALMEITCDNANRMGTASL
                     LFCHDTLLDGGQAAGFAVAMIDHPVPPPYKGIDDPMPQCIYRLKAAVRTLRSMSGELH
                     LDGQIGAIGFSRGGPMAAYLAVTNGRADLEGDGPHLDVPSDVQAALIHGNRYDYTDLS
                     DKDPMYKRFEKAWGPYDQNKQKWLDHGAA"
BASE COUNT         1117 a         1748 c         1678 g         1078 t
ORIGIN      
        1 ctagccgtcg ccgccaagcc agcgtatgtc gaaggtttgt acctccagca tcggaacgtt
       61 gaagcgctgc aggctgccga ccggtacctc gcccccggaa atgatttgcg gagcgaacaa
      121 cggtcgctag tgagctatta ccgagggatg atcctgttgg atttgggtcg cgtaaaggat
      181 gcgatagccg tcttgaaggc tgctacagag caaacgcgca cgcgaaaaat cgccattatc
      241 aatctttttg aggctcagtt gcgtgacgac gactgcgttg ccgctgaagc gacgctgaac
      301 gatattacga gaaactacca taatacgctg gggcgtttag cgaggctcaa agctgaactg
      361 gcgcacgcga aagaaacgca tggcatcctg cccctaaatg aggcgccgcg ggtctccaat
      421 agattgagcg acggcagcga acgtcgggag ccagtaatcg gtgtaacgga agatcggcat
      481 gccacgcgct agggtgatta gatcactttg gtgggttgcg ggaattggct acggtgctgc
      541 gctatctctg gctcctgcca tcacccacaa agaggcagcc ctgtccctgg caggtgcgtg
      601 gacgcggttg gtgattttcg tggctctcgg aggtattctt ttcggcatag tttggccaat
      661 agcgtggggc ctgctagtac agttattcgt tgccacacgc gtgccaccag gttcccgccg
      721 ttccgcggcg ggtgggccaa gtatgactgc aggaactgac agttctgacg gaagggcagg
      781 ggacgcggga caagttaccg atggtgtgaa tgcacccgac tgccaggtac attcgaatcc
      841 tgagaggcaa aatgttgttg cttcggtgcc agtcatgccc gacgagaagc cgatcgaaga
      901 gccgcagcgg ccggattcca agcagtttgc gggtgaatat tatccagtcg aacgaaggac
      961 acagacttac agtaagtcag gtccataata atagttcaaa cagctagagc cgcgttttca
     1021 tagaaacatg cttggtgatc cgtaaagccc gcgctcgccg tggggctcgg agatgccgaa
     1081 ccacatgctg ctgcgttgag tcgacatcgt attatcgaat ttcctttaca agtgtcgctc
     1141 cgtcgggcat cttgttaaca tgcaatgtct gggtgagatg ggtataaagt gaggcgcgtt
     1201 tagccatttt aatcaaaatt cccgcgcatt cattattgca aattgttatc atcaagcgag
     1261 ttgcgacaat tggcagcctg ctgagacacc gcaaaaactg tctcaaactg tctcattccg
     1321 cgcccatctt cacgtccgcc gaaccacgcg caggatcgga gccgcgagaa cgcccattcg
     1381 gcgaagctgg tattcgcacg cccagctggc tcccgtctgt cgccaggcgg cggggccgaa
     1441 ccagttgagc ttttcgttgg cgttgtgaac ggccccgaag gtgttcacac ggttgccaaa
     1501 cgcggtgatt tcgagcgacc gccgctggcg cgatcgacca atggtcagct cgaacggcgc
     1561 gaaggctatc tttccggcgg gtttgtcgtc gacgttcacc gacagaagcg gcgcgcgaaa
     1621 cttcggcacc gcgagggtaa ggtctcgatc atcaccatca atgacgcaac ggtaggtgac
     1681 gttgcccgcg taaaacggca agccttgccg tgtccagtcg ccgaaggcca attcgcgcac
     1741 gggtgcgatg atgcgagccg agcgaccggc gatggcgacg ccgaagtcgc cgagcagata
     1801 gcaccactcc aggttcgtct tccgcgtgaa cgggatcgtc aacagcaatt cgtgcacgtc
     1861 tgcggcgagc gcaggtagcg ctaccgtcgc aatcgattca tcgatccacc atccggtggg
     1921 gcgattggag acctcgtctg catcgaagtg aatcttcacc gtctcaggcg cctcgattgc
     1981 cagcgacggc tgatccaccg ttacctcact gcgaatgctg aaccgcagct gcagcagcgc
     2041 gaccaccggc gcttccgact gatccaccca cggctgcgcc atccgcccac cgcgcagcgg
     2101 gaggttcagc gcctggcgaa cgcgatcgtc gatgcgaagg acttcttccg ccgcgttcca
     2161 ctcgccgtcg ttcaaccgcc actcggcttg atccagcatc agtacgttcg gctccgacaa
     2221 cgtcaccggc accggacctt cgatccggcc gatctccacg agcttgggtt ccgcgaccgc
     2281 tcgaacagat cgccccgtgc gacggctcaa tcgaatcagc aaatgcccat gcggcgtgaa
     2341 gctccacggg atgacggtgt caccaacgtc gatcgtcgaa tacagcggtc gcgtgcggcc
     2401 agtcatcgag tccagctgat cgacatccca cttgccgcgc acgcggatgc gggcgtgccc
     2461 gcggccgaat tctcggtcgg tgttgcagaa gaagaggtaa acgtgcttgc cgtcatcacg
     2521 gcgttgatga aggatcgaat cggctcgcac gccgtcgccg tggtagattt cgacgtcgcg
     2581 aaatggcgac agtgccgcga ggatgtcccg ccggcgccat ccgacttggg tacatctcgc
     2641 cgctagtttc tgcgccgcgg gcgagcgatt gccgtccacc agtgatggga tttcgccggc
     2701 gaagatgacg gcgccgcctg cgttggcgaa acgctccagc cgctgcagcg tcgtcgcgcg
     2761 gatcgttcgc aggctcggca ccaggacagc gtcgtatgcc atttcgccga ccataaatcg
     2821 cttcgcatca cgctcgctgc cgcccgcctg cgtcggcagc aggctttcgc tgatgtagtc
     2881 gaagtcgatc aggccaaaga gcaaccagct cggcaactcg cgaaacgctt cctcccgatc
     2941 ctgcatctcc ggctggttct gttccatcga tccaaagctg aaccagtatg actcgatcgg
     3001 atggatcacc cccacgcgca cgtgcggccg gccgcgcgtc agcaccgtgt tcactcggcc
     3061 aaagtgatct tcgatgagcg gatactccgc ataccacggc gactgcggcc caatcgacgc
     3121 ggggtaatcg cgcttcgcct cgcccgccat cgacacccat gccaggtggg gcacgcgcac
     3181 cgtcacaccc atcgccgcct gccagtcgcc ttgcgctttg tgcccgacaa aatcgaaatc
     3241 ccagttggtg gcgccgtaca actcgctcag cacccccggc cggccgaact ggtgcgcgat
     3301 gctttgcgcc tgcttcgcgg tcgtcagctc catcttgtcc tgcaggatgt cgatgcccgg
     3361 caggtggaac ccgcggagcg cgcgcatgac ttcacccacg ccgcgcgtct gcgagaagag
     3421 gttcgcctcg ccgagcatgt gacccgtcag cgcgatgtga tgccgcccgc accactgccc
     3481 gagggtatcg ccaaacgcgg cggcgaagcg ctcggccgtg tgatcgtgaa agcgatatcg
     3541 cgccagtgac ggctcatcgt ccggtaaatt ccagaacaac tctggcaagt gatccagcaa
     3601 atcctgaccg tatgcgcgcc gataggtctc gacgaaatca tcggtgaacg gcatcaacac
     3661 gtcgcgctga tccgacgcca gctgaaatgt ctgcttcttc acgaactgcg gttcatccgt
     3721 aaaaatcgcc ggcaccagcg tgccgaaatg ctgacccacg gccgccgcgt agcgctcgtg
     3781 cgtgatctcg atgaaccgct cgatcgcccg gcggctgaac gtgtccacgt acgtctggcc
     3841 gttgaaccac gaatcctccg gcgtcgtttc cagatatgcg gcccatccgc cgcgcccgcg
     3901 cgcggccgca gatagtcgcc gatacttcgc cagccggccg tccttcagcg cgacggcgta
     3961 tcgcgccagc agcacgccgt cgccgcgata ttctttggac gtccacagca gccgcttggc
     4021 gcgaagcgat ttgtccttcg tcaccaaccc accggcgaag cccgatggcc agcggtcttc
     4081 gtcgtacaac cacgccagca tcgcccgtcg cttcgcgtgc tcggtgcagg cgatcaccgc
     4141 gtcgaaaaat tcccggccga gatactccgt ctccatgccc gtccgcgggt gcatgtggaa
     4201 gccgccgaag cccatcgctt tgagttgatc gatctgccgg agcagttgcg ggatgcacag
     4261 tcggttgttc cacgaccaga acggcgtgcc gcgatattcg gcggtggggc ggcggaacag
     4321 ggcgcggtcc aactcgcgcg aatcgttttg cggatacagc tggaatgtcc gacgccgaga
     4381 gcccattcgt ctgtgatggt atccgagacg gcatgatcta tctggtgcgc gggccaactg
     4441 cggaggataa attgaaagcg aattgatgct ttgtgtaact gacgaagcaa gcgggattgg
     4501 caatgatcat gggcgtgatg gagtcgatga aaaatgctcg ccgcagccgc actgctcagt
     4561 cttcatcgct tgtgggcgaa cgggcagagc cccctgcgtc tgcatgtttg ttcttgcttt
     4621 ttctctgtac cgtacccgcg gccgttcgcg tgaacgcggc gcctgaacca accacacaat
     4681 ccgcgaacag gtcgattcaa tggcaaagtc agactaccgg cactgcgctc accggtatcc
     4741 tgaagccgcc cgccactccc gcgcgcgccg ccgatggcag tgtcgcgacc gtcatctatc
     4801 tgaagaatct ttccatccct cgtctcggcc aggagccgga cgaaccaatc tttcgcgacc
     4861 tatcggaagc gggaaatctc gtgttgatcc tcgattatgc acacgatccg cacgccacat
     4921 ctccggccat caacgcggac atgcttaagc tgcggcagga catcggcggc aagagcaagt
     4981 cgctgctcgc cgatcagaag gtggatctca gtcacctgtt cattctgatc gaaggttttc
     5041 gcctgaagcg agacgtcgcg ttcgccaagg atggctcgcg cacgcttgcg atggatgtgc
     5101 agtatccatc gcagccgacg catcccgtgc cggcgctgat ggagatcacc tgcgacaacg
     5161 ccaaccgcat gggcacggcg tcgctgctgt tctgccacga cacgctgctg gacggcgggc
     5221 aagccgcggg gttcgcggtg gcgatgatcg accacccggt gccgccgccg tacaagggga
     5281 tcgacgaccc gatgccgcag tgcatttatc gactgaaggc cgccgtgcgc acgctgcggt
     5341 cgatgagcgg cgagttgcat ctcgacggcc agattggcgc gatcggtttt tcgcgcggcg
     5401 ggccgatggc ggcgtacctc gcggtcacca acggccgggc cgatctcgaa ggtgatggtc
     5461 cgcatctcga tgtcccgagc gacgtgcagg ccgccctcat ccacggcaac cgttacgatt
     5521 acacggacct gagcgacaag gacccgatgt acaagcggtt cgaaaaggcg tggggaccat
     5581 acgaccagaa caagcagaag tggctcgacc acggggcggc g
//
LOCUS       DATIMH010000329         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4113712, whole genome shotgun sequence.
ACCESSION   DATIMH010000329 DATIMH010000000
VERSION     DATIMH010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4113712"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VLI90_05600"
     CDS             <1..248
                     /locus_tag="VLI90_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13713.1"
                     /translation="CTRPMKDILFDEKIAGSIHFTPGNCYDEASNGNKSGIHWDMVMR
                     QDPQVGGGEIWFDDKLVRKDGRFVVKELEGLNPENLK"
     gene            458..646
                     /locus_tag="VLI90_05605"
     CDS             458..646
                     /locus_tag="VLI90_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13714.1"
                     /translation="MARPTRRTLARVLVLLRQRYGPRPTKIWGDGVSVLVDTILSQNT
                     SAKNSDAGYRQLRRRFPS"
     gene            653..1144
                     /locus_tag="VLI90_05610"
     CDS             653..1144
                     /locus_tag="VLI90_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13715.1"
                     /translation="MANAPAAEVERHIRVSGLSNQKAPRIQSILRQIKQRHGKVDLQF
                     LAKMDDDAAFEYLMQFHGVGPKTANCTLLFAFGKPLFPIDTHIHRIARRLGLIGAKAT
                     SEQAHALLKPLIAPADRYETHVLLIEHGRRTCKAINPRCDDCVLLRMCPFGETRVRAN
                     EYS"
     gene            complement(1271..2005)
                     /locus_tag="VLI90_05615"
     CDS             complement(1271..2005)
                     /locus_tag="VLI90_05615"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSV13716.1"
                     /translation="MAGHSHWAKIKRAKGANDKKRGKIWSKIARKIIIAAKNGGDPRD
                     NLTLRYTIDEAKAANMPKDTIANAVKKGTGELGGENYEAVTYEGYGPAGVALIVEGLT
                     NNRARTAPEMRKIFEVNGGNLATSGAVAFQFNKRGVITIKSDATTEDRLMEIALEAGA
                     EDVTNEGEVFEVITSPIQFLTVRDAISAANIPIEASEITNLPTSSIAVDAEVGQRVLK
                     LIDALEDNDDVQSVAHNAELPESIVA"
     gene            complement(2043..>2387)
                     /locus_tag="VLI90_05620"
     CDS             complement(2043..>2387)
                     /locus_tag="VLI90_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV13717.1"
                     /translation="NESFLDSLGWVLYKRGKFTEARKYLEQAVGPAAFPDPVVLDHLA
                     DTLYRLSQNADATKLWQRSLKGIGEGEPDRDDLKQLRIQLIQKIKDAEANKPVEVAPT
                     VEGTTSTAQAKQ"
BASE COUNT          430 a          723 c          735 g          499 t
ORIGIN      
        1 actgcaccag gccgatgaag gacatcctgt tcgacgagaa gatcgccggc agcatccact
       61 tcacgccggg caactgctac gacgaggcga gcaacgggaa caagagcggc atccactggg
      121 acatggtcat gcgccaggac ccgcaagtgg gcggcggcga aatctggttc gacgacaagc
      181 tagtccgcaa agacgggcgg tttgtggtga aggaactgga agggctgaat cccgagaatc
      241 taaaatgagg aatgaatctc acgtccataa ccttgaccag aaaacgaact tctgtcgcgg
      301 aacagcgacg gacgcagcgc catcatcagc gccgctcgtt gaattcgatt gttcggcaat
      361 tgaggcgctc attctataaa cgccatttgg tcggccttgc cgctgatcga aacgtcggac
      421 gagaggtctg gtagaagcac ccacgaagta ctcgtcaatg gcccgtccca ctcgccgcac
      481 cctcgcccga gttctcgtcc ttctccgcca acgatacggc ccgcggccga caaagatctg
      541 gggcgatggc gtcagcgtgc tggtcgacac catcctctcg caaaatacgt cggcgaagaa
      601 cagcgacgcg ggatatcgcc aactgcgccg gcgattcccg tcgtgaaacc aggtggcgaa
      661 cgcgccggca gcagaagtcg aacgtcatat ccgcgtcagc ggattgtcga accagaaagc
      721 gccgcgcatc cagtcgatcc tgcggcagat caagcagcgg catggcaaag tcgacctgca
      781 gttcctcgcg aagatggacg acgacgccgc gtttgaatac ttgatgcaat tccacggcgt
      841 cgggccgaag accgccaact gcacgctgct gttcgcgttt ggcaagccgc tgttcccgat
      901 cgatacgcac atccaccgca tcgccaggcg gctgggcctc atcggcgcga aggcgacttc
      961 ggagcaagcc catgctctgc tcaagccgct gatcgcgccg gcggatcgat atgaaacgca
     1021 cgtcctgctg atcgaacatg gccgccgcac gtgcaaggcg atcaatccgc gatgcgatga
     1081 ctgcgtgctg ctgcgcatgt gcccgttcgg agaaacgcgc gtgcgcgcga atgaatattc
     1141 atagccgcag gcgactgcca gcgggtgcag cgcaattgag cggaacgcac gcagccaagc
     1201 tgcgaaagcg cccgcgggct gtcgcctgcg gctgatatgg ttgagggatc tcgcgctcgc
     1261 ggcgcacacg tcacgcgacg atggattcag gaagctctgc gttatgcgcg acgctctgca
     1321 cgtcgtcgtt gtcttccagc gcgtcgatca gcttcaagac acgctggccg acctcggcgt
     1381 ctaccgcgat actgctggtt ggcaggttgg tgatttccga tgcctcgatg ggaatgttgg
     1441 cggcgctgat cgcgtcgcgc acggtcagga attggatggg cgatgtgatg acctcgaaca
     1501 cttcgccctc gttggtcacg tcttcggcgc cggcttccag cgcgatttcc atcaggcggt
     1561 cttcggtcgt ggcgtcggac ttaatcgtga tcacgccgcg cttgttgaac tggaacgcga
     1621 cggcgccgct ggtggctagg ttgccgccgt tcacttcgaa gatcttgcgc atttcgggcg
     1681 cggtgcgggc gcggttgttc gtcagcccct cgacgatcag cgccaccccg gcggggccgt
     1741 agccttcgta ggtgacggct tcgtagtttt ccccgccgag ttcaccggtg cccttcttga
     1801 ccgcgttggc gatcgtgtcc ttgggcatgt tggcggcctt ggcttcgtcg atcgtatagc
     1861 gcagggtgag gttgtcgcgc gggtcgccgc cgttcttggc ggcgatgatg atcttgcggg
     1921 cgatcttgct ccatatcttg ccgcgctttt tgtcgttcgc gcctttggct cgcttgatct
     1981 ttgcccagtg actgtggccg gccatgtgct tgcttgctcc gggtcgtaaa cctatttagt
     2041 tgctactgct tcgcttgcgc tgttgatgtc gtaccttcaa ccgtcggggc gacctccacc
     2101 ggcttgttcg cttcggcatc cttgatcttc tggatcagct ggatgcgcag ctgcttcaga
     2161 tcgtctcgat ccggctcgcc ctcgccgatg cctttgaggg accgttgcca caatttcgtg
     2221 gcgtcggcgt tttgcgacag gcgatagagc gtatccgcca ggtgatccag cacgaccggg
     2281 tcggggaatg ccgccggacc gaccgcttgt tccaggtatt tccgcgcctc ggtgaatttt
     2341 ccgcgcttgt acaacaccca gccgaggctg tcgaggaacg attcatt
//
LOCUS       DATIMH010000330        10392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4983024, whole genome shotgun sequence.
ACCESSION   DATIMH010000330 DATIMH010000000
VERSION     DATIMH010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 10392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10392
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4983024"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2047)
                     /locus_tag="VLI90_05625"
     CDS             complement(<1..2047)
                     /locus_tag="VLI90_05625"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HSV13718.1"
                     /translation="MRCESRDVPMGIDTTHPRLSWTLASDERGQRQSAYQIQVTSSAG
                     RFDTPDLWDSGKVSSDESASIAYAGKPLNSLATCAWRVRVWDVNDHPSSWTDGPAWTM
                     GLLAPSDWQAKWIDRGNGPAATQPTTPMPRFSKTFRAAQPVKRALIAICGLGQFDLRI
                     NGKPARDDFLQPGWTDYRKTCLYVLYDVTPLLQRDQNVVSVTLGNGMYNCVGGRYTKF
                     KASFGPPKLIAQLRLDYADGTSQTIGTDSSWKTGDSSTTFSSIYGGEDVDARISSDES
                     ASSSAHETDGPGGTLACASKSALPIQIDHVLTPVKITEPRPGVFVYDMGQNCAQVPQL
                     RVSGPAGTKVVLEPSELLHPDGMIAPQSTGAKLGGVHSTYTLRGGGGVETFEPRFYYA
                     GCRYWQVTGAIPKDSAGADDPTKAVVQELRGQFVTSTSPIAGEFECSSEMFNRTRTLI
                     RWAMRSNMMSVLTDCPHRERLGWLEQDHLVGPSITYNFDVQALLGKICNDMAEAQHDD
                     GLMPDIAPEYVKFKGGFLDSPEWGSACVLVPWQAYQSYGDEQVLRDHLDTMTRYVEYL
                     NRQSKEHILDFGLGDWYDLGPKPPGIAQLTPKALTATAFFYRDASLVAQIAKLLGRAE
                     DATKYASLAGEICEAFNRAFYDAAHHRYATGSQTGDALPLVFGMVPETDRAAVLDDLV
                     "
     gene            complement(2247..2807)
                     /gene="thpR"
                     /locus_tag="VLI90_05630"
     CDS             complement(2247..2807)
                     /gene="thpR"
                     /locus_tag="VLI90_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HSV13719.1"
                     /translation="MRLFLAIELPRVVREHLRGLQAIVAEYAPKAALVPTENLHLTIK
                     FLGEVDDRRLAALEESLEKLHRVGVIMLQAQDLECFPLRGPIRIIGAAVGGDLTPLLA
                     LQKAVEQRCQYLGFDTEARGFRPHITLARAKAGLPPAARATLTLAAQSSWPGPTFTID
                     HFTLMRSVLKPKGAEYHAVRTFTTIA"
     gene            2893..4188
                     /locus_tag="VLI90_05635"
     CDS             2893..4188
                     /locus_tag="VLI90_05635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSV13720.1"
                     /translation="MPKEADVLIVGAGVAGLTAGRRLSGAGRRVMVLEARDRIGGRIH
                     TFHDPASPVPIERGAEFIHGVPTETWDIVRAGKLLVADTSDEHWHFADGRLHKRDDLW
                     EQLQRVMDRVKVLGDRDMPFAQFIREHVREPELHEAADLATAFVEGFDAAHADRISAQ
                     ALKRENEASQNEQEDRQFRLLNGYDGLVNWLAAGIAPPSDVRLRTAARTIRWSRDRVQ
                     IETDGEMFTAARAIVTLPLGVLQLAEGDPSAVRFDPPLPEKLDAARRLAMGPVVKIFL
                     RFRESFWERDERLRTMSFVHSRELCFPTWWTFLPVRAPALTGWAGGPAADRLSGRGDA
                     FILDKALETLAKIFDRPRREIESLLEQSIVSDWPSDPLSRGAYSYVPAGSGDAMRQLA
                     AAVQQRLFFAGEATHYEGQSGTVAGAIASGYRAASEVLG"
     gene            4427..4720
                     /locus_tag="VLI90_05640"
     CDS             4427..4720
                     /locus_tag="VLI90_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13721.1"
                     /translation="MPRGDKSSYTNKQKRQAEHIEEGYEKRGTSKKEAERRAWATVNK
                     ETGGGKRSGSGRGKAVDRSSSRKGGHKGGSSQSHATRVRAGKKAARTRAKHGK"
     gene            4829..6211
                     /locus_tag="VLI90_05645"
     CDS             4829..6211
                     /locus_tag="VLI90_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HSV13722.1"
                     /translation="MTDTTGVFAAKTAGPIGAAKTAENPASTCPADPSPQKKTSFFKR
                     LGPGLITGASDDDPSGIATYSQAGAAYGYDLLWTLLLTLPLMAAIQEIAARIGRVTGR
                     GIAGNMRRHYSAWLLYPVISLLVIVNTINLGADIGAMGDAVKLLVGGPALVYAAILAI
                     ISVLLQVFASYPRCTKFMKWLTLALFSYVVSVFIVHLPWGQALWRTIVPHWSFSGDYW
                     ATVIALLGTTISPYLFFWQASQETEEIRANGEQKPLKREPCQAPLQLERIRLDTYVGM
                     TFSNLVAYCIILAAAATLHGNGITEINTSADAAKALEPVAGRFAFVIFAIGIVGTGML
                     AVPVLAGSAAYGVGEALKWRTGLDQKPRRAKGFYGVLTAATFIGLALNVPAVQHVTHL
                     SPIKALFWAAVINGVVAVPIMVVMMLMFHNGKVMGEFAKGSRGLKVLGWISTAAMFVA
                     SLGLFLTWKS"
     gene            complement(6396..7061)
                     /locus_tag="VLI90_05650"
     CDS             complement(6396..7061)
                     /locus_tag="VLI90_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3826 domain-containing protein"
                     /protein_id="HSV13723.1"
                     /translation="MKNHFCKACVVVAVVSIMSVSIPAWSAKKPAATQDSADQTAAYA
                     ATANERAAKIVKSLKIEDEAKAARVRDAIAAQYIALNGIQTANEAAGLKPAQAAKDPA
                     KQQALKNLHEQFIAKLEADLAPEQVEQVKDGMTANKVRVTYDAYNKFIPNLTGEENAK
                     IMGWLKEARELALDAGSMEAKSEIFNKYKGRINNYLASHGKSANPSKKPPATRAADLP
                     AAD"
     gene            complement(7058..9397)
                     /locus_tag="VLI90_05655"
     CDS             complement(7058..9397)
                     /locus_tag="VLI90_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3826 domain-containing protein"
                     /protein_id="HSV13724.1"
                     /translation="MKSFAYCIALFLALAVATNARGQTSQPVYTLPPGQVQYKKGYPA
                     IPPEIKAADEARRREWKRLDDDAWHRAEPDVARWAANGKPYIALALNPDDLPQAEIPA
                     FPGAEGGGMYTVGGRGGRVFVVTSLADSGPGTFREACEAGGPRIVVFNVAGIIHLDRP
                     IHIRAPYITIAGQTAPGDGICVAGRSTKIDTHDVVVRYMRFRRGITELADRDDALGGD
                     APIGNIIIDHCSCSWGLDETLSIYRQMYPTVPGDESKRLKLPVVNITIQWTIITEGLD
                     TYHHAFGATWGGRNSLFSHNLFACNTGRNASIGMNYDFNWINNVVFNWRHRTLDGGDD
                     LSLVNCINNYYKPGPVTNDSPVKYRVLLPQPKRTKDPKAPRSFGRYYVEGNVVDGNER
                     VTANNWDGGVQFPGGAGNDTGDNGSVTGDQAKQLIEQVRLTQPLPMAPVTIQPAKDAY
                     EAVLAGGGATLPRRDSVDQRAVEQTRTGKVQYDKGIITDISQVGGYPQYAGEPVKYSQ
                     NDGIPDWWKAKYHLDVNDRQLASKDAGDGYTYIEKYLDGLDPTVKVDWKDLKNDVNTL
                     GDGAALRAEPGARSTKTPEQIEAKYEDSLNRRANDILSAIGVIDAAKAARVHDALVEH
                     WRAVKAYHESPGAALVKVEGMKAKIPDPADPAVRAMHDKLIAALSADLMPEQVSTVKD
                     KLTVGKLEFTYKGYEAIVPNLTDTERAKIRELLTAAREEAIDARDTDAKSAVFKKYKK
                     QIQAYLNDNGRNWDQMYKEYAVAVKAKKAAAASQPAVEQ"
     gene            complement(9469..>10392)
                     /locus_tag="VLI90_05660"
     CDS             complement(9469..>10392)
                     /locus_tag="VLI90_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13725.1"
                     /translation="HDLFACNTGRNPSIAMSYDFNFVNNVLFNWRHRSVDGGDEHSQI
                     NMINNYYKPGPATPDSPVRYRIVEPAQSWSKANPVSRWGKVYAAGNVVEGNEKVTKNN
                     WDGGVQFQFAPDRDASGAIANGAIRDDDKLKEIIAKVREDKPFPMPPMTIQSAEDAFQ
                     SVLANAGATLPRRDAVDQRVIEEVRTGVTWGMGKEIPIEPMKDLAKNNIGVAGNGIIT
                     DVNQVGGYPEYKGEPVKYTQNDGIPDWWKQKYHLDVNDPDLAGKDCNGDGYTNIEKYL
                     NGLEPTQKVDWHDLKNNVNTLAPAQLTASVK"
BASE COUNT         1697 a         3334 c         3286 g         2075 t
ORIGIN      
        1 tgaccaggtc gtccagcacc gccgcgcgat cggtctcggg caccatgccg aagaccagcg
       61 gcagcgcatc gcccgtctgg ctgccggtgg cgtagcggtg atgcgccgcg tcgtagaacg
      121 cgcgattgaa cgcttcgcag atttcgccgg cgagcgaagc gtacttcgtc gcatcctccg
      181 cgcggccgag cagcttcgcg atctgcgcca ccaggctcgc gtcgcgatag aagaacgccg
      241 tcgccgtcag cgccttcggc gtcagctgcg cgatgcccgg cggcttgggg ccgaggtcgt
      301 accagtcgcc caagccgaaa tcgagaatgt gctccttcga ctggcgattc agatattcga
      361 catagcgcgt catcgtatcc aaatgatcgc gcagcacctg ttcgtcgccg tacgattgat
      421 acgcctgcca cggcaccagc acgcacgcgc tgccccactc gggcgaatcc agaaacccgc
      481 ccttgaactt cacatactcc ggcgcgatgt cgggcatcag cccgtcatcg tgctgcgcct
      541 cggccatgtc gttgcagatc ttgccgagca gcgcctgcac gtcgaagttg tacgtgatcg
      601 acggcccgac gagatggtcc tgctccagcc aacccagccg ctcgcggtgc gggcagtcgg
      661 tcagcacgct catcatgttg ctgcgcatcg cccagcgaat cagcgtgcgg gttcggttga
      721 acatctcgct ggaacattcg aattcgccgg cgatgggcga ggttgacgtg acgaactgcc
      781 cgcggagttc ttgcacaacc gccttggtcg gatcgtccgc gcccgccgaa tctttcggga
      841 ttgcgccagt cacttgccaa tagcggcaac cggcgtagta gaaccgcggt tcgaacgtct
      901 ccaccccacc cccgccgcgg agcgtgtagg tgctgtgcac gccgcctaac ttcgcgccgg
      961 tgctttgcgg cgcgatcatg ccgtcgggat gcaacagttc cgacggctcc agcaccacct
     1021 tcgtccccgc cgggccgctc acgcgaagct gcggcacctg cgcgcagttc tggcccatgt
     1081 cgtagacgaa cacgcctggc ctgggctcgg tgatcttcac cggcgtgagc acatgatcga
     1141 tttggatcgg cagcgccgac ttcgacgcgc acgccagcgt gccgccggga ccgtctgttt
     1201 cgtgcgcgct gcttgacgcg gattcatcgc ttgagattcg agcatccaca tcttcgccgc
     1261 cgtagatgct gctgaacgtt gtggatgaat cgccggtctt ccacgatgaa tcggtgccga
     1321 ttgtctgcga tgtgccgtca gcgtaatcca ggcgtagctg cgcgatgagc tttggcggtc
     1381 cgaacgatgc tttgaatttc gtgtagcgcc cgccgacgca gttgtacatg ccgttgccga
     1441 gcgtcacgct gacgacgttc tggtcgcgct gcagcagcgg cgtgacatcg tacaggacgt
     1501 acaggcacgt cttgcgataa tcggtccagc cgggctgcag gaaatcatcg cgggccggct
     1561 tgccgttgat ccgcaggtcg aactgtccga gcccgcagat cgcgatcagc gcccgcttca
     1621 ccggctgggc cgcgcgaaat gttttcgaga accgcggcat cggcgtcgtc ggctgcgtcg
     1681 ccgccgggcc gttgccgcga tcgatccact tggcttgcca gtcgctcggc gcgagcagcc
     1741 ccatcgtcca ggccggcccg tcggtccaag acgacggatg atcgttcaca tcccacacgc
     1801 gcacgcgcca ggcgcacgtc gccaacgaat tgagcggttt cccggcgtag gcgatcgacg
     1861 cggactcgtc cgacgacact tttccgctgt cccacaggtc cggcgtgtcg aagcgaccgg
     1921 ctgacgacgt gacctgaatc tggtacgccg actgccgctg cccgcgctca tcggacgcca
     1981 gcgtccagct caaccgcggg tgcgtcgtgt cgatccccat cggcacgtcg cggctctcac
     2041 agcgcaagcg cgtgatccgg aatggttcgc ccgccgcgga tgccgaagtc gtcaacgcca
     2101 ttgaaatcaa cacgagaaag gtgaggcaat tacgcatcgg cgggatctta cccggccgag
     2161 gttttcacgg gaacgcagcc gcgacgcctg cgtcgccttg gctccaagag gcgacatagg
     2221 cgtcgcggct acggggaccg tgcggtctac gcgatcgtcg taaacgtccg gaccgcgtga
     2281 tattccgcgc ccttcggttt aagcacgctc cgcatcagcg tgaagtgatc gatcgtgaac
     2341 gtcggccccg gccaactgga ttgcgcggcc agcgtcaacg tcgctcgcgc cgccggcggc
     2401 agacccgcct tggcgcgggc gagcgtgatg tgcggccgaa agccgcgggc ttcggtgtcg
     2461 aagccgagat actggcagcg ctgctcgacc gccttctgca gcgccagcag cggtgtgaga
     2521 tcgccgccga cggcggcgcc aatgatgcgg atcggcccgc gcagcgggaa acattccagg
     2581 tcctgcgcct gcagcatgat cacgccgacg cgatgcagct tttccaacga ctcttcgagc
     2641 gccgccagcc ggcggtcgtc cacctcgccc aggaacttga tcgtcaggtg caggttctcc
     2701 gtcggcacca gcgcggcctt cggcgcatac tcggcaacga ttgcctgcag cccgcgaaga
     2761 tgctcgcgga cgaccctcgg caactcgatg gcgaggaaca ggcgcatggc tcgaaccgct
     2821 tcatgccccg cgcgcggcca gttgaatatt tgccgttgcc tgcgaggctg actggcagcg
     2881 tacaacattg tcatgccgaa ggaagcggac gttttgatcg tcggcgccgg cgtcgccggt
     2941 ctgaccgcgg ggcgacggct gtccggcgcc ggccggcgcg tgatggtcct cgaagcgcgc
     3001 gaccgcatcg gcgggcgcat tcacacattc cacgacccgg cctcgcctgt gccgatcgaa
     3061 cgcggcgcgg agttcatcca cggcgttccc accgaaacct gggacatcgt ccgcgccggg
     3121 aaactactgg tcgccgacac gtccgatgaa cattggcact tcgccgatgg ccggcttcac
     3181 aagcgcgacg atctttggga gcagcttcag cgcgtgatgg atcgggtgaa ggtgctcggc
     3241 gatcgcgaca tgcccttcgc gcagttcatc cgcgagcacg tgcgcgagcc ggagcttcac
     3301 gaggcggccg acctggcgac ggcgttcgtc gaaggtttcg acgccgccca cgcggatcgg
     3361 atcagcgcgc aggcgctgaa gcgggagaac gaggcgagcc agaacgagca ggaagatcgg
     3421 cagttccgat tgctcaacgg ttacgacggc ctggtgaact ggctggccgc cggcatcgcc
     3481 ccgccgtctg acgtgcgatt gcgcacggcg gcgcggacga tccggtggtc gcgcgatcgc
     3541 gtgcagatcg aaacggacgg cgagatgttc accgcggcgc gggcgattgt cacgctgccg
     3601 ctgggcgtgc tgcagttggc ggagggcgat ccatcggccg tgcgtttcga tccgccattg
     3661 ccggagaaac tagatgccgc ccggcggctg gcgatggggc cggttgtgaa aatctttttg
     3721 cgctttcgcg aatcattctg ggagcgcgac gagcggctgc ggacgatgtc gttcgtgcat
     3781 tcgcgcgagt tgtgtttccc gacgtggtgg acgttcctgc cggtgcgcgc gccggcgctg
     3841 accggttggg ccggtggtcc cgccgccgat cgactatcgg ggcgcggcga cgcgttcatc
     3901 ttggacaagg cgctcgaaac gcttgccaag atcttcgatc gcccgcggcg cgagatcgag
     3961 tcgctgctcg agcagtcaat cgtgtccgat tggccgagcg atccgctgtc gcgcggcgcg
     4021 tacagttacg tccccgccgg cagcggcgat gcgatgcgcc agctggcggc ggcggtgcag
     4081 cagaggctgt tcttcgccgg tgaagcgacg cattacgaag gccaatccgg cacggtcgcc
     4141 ggcgcaatcg cgagcggata tcgcgcggcc agtgaggtgc ttggatgagc gctggtggcc
     4201 ggaggagcgg aattcaacca cgaagacacg aagagcacga agggggagac accgaagtgc
     4261 gtgcgatgca cgacgttcaa acgatctcgt tttacatttt tgcagttcca tccgtttcgt
     4321 tgcgcgcgtt atcgaagagc catgcccgtc ggtttaactc gccacggccg gtgggattca
     4381 tcttcgttgg cgagacgagt gtacgcagcc aagtggaggc tccaccatgc cacgaggaga
     4441 caagtcaagt tacacgaaca agcagaagcg ccaggcggaa cacattgaag aggggtacga
     4501 gaagcgcggg acgtcgaaga aggaggccga acgccgcgcc tgggcgacgg tgaacaaaga
     4561 gaccggcggc gggaagcgca gcgggtccgg tcgcgggaag gcggttgacc gttcgtcctc
     4621 gcgcaagggc gggcataagg gtgggtcgtc gcagtcgcac gcaacgcgcg ttcgggcggg
     4681 gaagaaggcg gcgcgcacgc gggcgaaaca tggcaagtag cgtgatcttg cagccgcttg
     4741 cggattggtt ttgcgaagcc gcaagcggcg gttttttcac catttctgcc gtgccaccaa
     4801 tcgatcggcg tcgatacctt atcaggtgat gactgatacg acaggagtct tcgcggccaa
     4861 gacggctgga ccgattggcg cggcgaagac ggctgaaaac cctgcctcta cctgtcccgc
     4921 agatccatca ccgcagaaga aaacaagctt cttcaaacgc cttggccccg gtctgatcac
     4981 cggcgcatcg gatgatgacc ccagcggcat cgcgacgtac tcgcaagccg gggcggcgta
     5041 cggttacgac ctgctctgga cgctgctgct gacgttgccg ctgatggcgg cgattcagga
     5101 aattgcggcg cgcatcggcc gcgtgaccgg ccgcgggatc gccggcaaca tgcggcggca
     5161 ttactcggcg tggctgctct atccggtcat ctccctgctg gtgatcgtca acacgatcaa
     5221 cctgggcgcc gacatcggcg cgatgggcga cgccgtgaag ctgctggtcg gcgggccagc
     5281 gctggtttat gcggctatcc ttgccatcat cagcgtcctc ctgcaggtct tcgcgtcgta
     5341 tccgcgctgc acgaagttca tgaagtggct gacgctcgcg ctgttcagct atgtggtgag
     5401 cgtcttcatc gtgcacctgc catggggaca ggcgctgtgg cgaacgatcg ttccgcactg
     5461 gtcgttctcc ggcgattact gggccaccgt catcgcgctg ctgggcacga cgatcagccc
     5521 ctacctgttc ttttggcaag cctcgcagga gacggaagag attcgcgcca acggtgagca
     5581 gaaaccgctg aagcgcgagc catgccaggc gccactgcag ctggaacgca ttcgcctgga
     5641 cacctacgtc gggatgacgt tctcgaacct cgtcgcgtac tgcatcatcc tcgccgccgc
     5701 ggcgacgctg catgggaatg gcatcaccga gatcaacacc tccgccgacg ccgccaaggc
     5761 gctggaaccg gtcgccgggc gattcgcgtt tgtgatcttc gcgatcggca tcgtcggcac
     5821 cggcatgctg gcggtgcccg tgctggccgg gtcggcggcg tatggggtgg gtgaagcgct
     5881 gaagtggcgc acgggacttg atcagaagcc ccgccgcgcc aagggttttt acggcgtcct
     5941 caccgcggcc acattcatcg gcctggcgct gaacgtcccg gcggtgcagc acgtcacgca
     6001 cctgtcgccg atcaaagcgc tgttctgggc cgccgtcatc aacggcgtcg tcgcggtgcc
     6061 aatcatggtc gtgatgatgc tgatgttcca caacggcaaa gtgatgggcg aattcgccaa
     6121 aggctcgcgc gggttgaagg tgctggggtg gatttcaacc gctgcgatgt tcgtggcatc
     6181 gctgggattg tttttgacgt ggaagagtta gcgcacgcgg tcctcctgga aaatggcatt
     6241 ccagccgctt gcggcttagc gcctgttcaa gtgccaaaac agacgccaag ccgcaagcgg
     6301 ctggaactgc gcgagtgaag gttcacctgc gtcccggcta gttagatcct tcgactacgc
     6361 acgcgtacgt gcttcgctca ggatgacaag ttgctctaat ccgccgcagg aagatcggcc
     6421 gctcgcgttg ccggcggttt cttcgacgga ttggcgctct tgccgtgcga ggcgagatag
     6481 ttgttgatcc ggcccttgta cttattgaag atctcagact tcgcttccat gctgccggcg
     6541 tccagcgcca gctcgcgcgc ttccttcagc cagcccatga tcttcgcgtt ctcttccccg
     6601 gtcaggttcg ggatgaactt gttgtaagca tcgtacgtca cccgcacctt gttggcggtc
     6661 atgccgtcct tcacctgctc gacctgctcg ggcgccaggt cggcctcgag cttcgcgatg
     6721 aactgctcgt gcaggttctt cagcgcctgc tgcttcgccg ggtccttcgc cgcctgcgcg
     6781 ggcttgagcc ccgcggcttc attggcggtc tggatgccgt tcagcgcgat gtattgagcg
     6841 gcgatcgcat cgcgcacgcg agcggccttg gcttcatctt cgatcttcaa cgacttgacg
     6901 atcttcgccg cccgctcgtt cgcggtggcg gcgtatgccg cggtctgatc ggctgaatcc
     6961 tgcgtggccg ctggcttctt cgccgaccat gcggggatgc tcacagacat gatcgacacg
     7021 accgcgacga cgacgcaggc tttacaaaaa tggttcttca ttgttccacc gccggctgcg
     7081 atgccgccgc cgccttcttc gctttcacgg ccacggcata ctccttgtac atctgatccc
     7141 agttccggcc gttgtcgttc aggtacgcct ggatctgctt cttgtacttc ttgaagactg
     7201 ccgactttgc gtcggtgtcg cgggcgtcga tcgcctcttc acgcgcggcc gtcagcagct
     7261 cgcgaatttt cgcccgctcc gtgtccgtca ggttcggaac aatcgcttcg taacctttgt
     7321 acgtgaactc cagcttgccg accgtcagtt tatctttgac ggtcgacacc tgctccggca
     7381 tgaggtcggc cgacagcgcg gcgatcagct tgtcgtgcat cgcgcgaacc gcggggtccg
     7441 ccgggtcggg aatttttgcc ttcatcccct cgacctttac cagcgccgcg cccggcgact
     7501 cgtgatacgc cttcaccgcc cgccagtgct cgaccagtgc atcgtgcact cgcgccgctt
     7561 tcgccgcatc gatcacgccg atcgcgctca ggatgtcgtt cgcccggcga ttcagagagt
     7621 cttcgtattt cgcctcgatc tgctccggcg tcttcgtcga acgtgccccg ggctcagcgc
     7681 gaagcgccgc gccgtcgccc agcgtgttca cgtcgttctt cagatctttc caatccacct
     7741 tcaccgtcgg atccagacca tcgagatact tctcaatata cgtgtaccca tcgccggcgt
     7801 ccttgctggc cagctgccgg tcgttcacgt ccaggtgata tttcgccttc caccaatccg
     7861 gaatgccgtc gttctgcgaa tacttcaccg gctcgcccgc gtactgcgga tatccgccga
     7921 cctggctgat gtcggtgatg atgcccttgt cgtattgcac cttcccggtg cgggtttgct
     7981 ccaccgcgcg ctgatcgacg ctgtcgcgcc gcggcaacgt cgctccgccg ccggccagca
     8041 ccgcttcgta tgcatccttc gccggctgga tcgtcaccgg cgccatcggc agcggctggg
     8101 tcaatcgcac ttgctcgatc agctgcttcg cttgatcgcc ggtgacgctg ccattgtcgc
     8161 cggtgtcgtt gccggcgccc ccggggaact gcacgccgcc gtcccagttg ttggcggtga
     8221 cgcgctcatt gccatcgacg acgttgcctt cgacgtaata ccggccgaac gaccgaggcg
     8281 ctttcgggtc tttggtgcgc ttcggttgcg gcagcagcac gcggtatttc acgggtgaat
     8341 cgttggtgac cgggccgggt ttgtaatagt tgttgatgca gttgacgagc gacaggtcat
     8401 ccccgccgtc cagcgtgcgg tgccgccagt tgaacacgac gttgttgatc cagttgaagt
     8461 cgtagttcat gccgatgctg gcgtttcgac cggtgttgca ggcgaagagg ttgtgcgaaa
     8521 agagcgaatt ccgcccgccc caggtggcgc cgaacgcgtg gtgatacgtg tcgaggccct
     8581 cggtgatgat cgtccactgg atcgtgatgt tgacgaccgg cagcttcagc cgcttgctct
     8641 catcgcccgg cacagtcggg tacatctggc gatagatcga gagcgtttcg tccaggcccc
     8701 agctgcacga gcagtggtcg atgatgatgt tgccgatcgg cgcatcgcca cccagcgcat
     8761 cgtcgcgatc cgccagctca gtgatcccac ggcggaaacg catgtagcgc acgacgacat
     8821 cgtgcgtgtc gatcttggtc gatcgcccgg cgacgcagat gccatcgccg ggcgcggtct
     8881 ggccggcgat ggtgatgtac ggcgcgcgaa tgtgaatggg tcgatcgaga tggatgatgc
     8941 cggcgacgtt gaagacgacg atgcgcggcc cacccgcttc gcacgcttca cgaaacgtgc
     9001 ccggcccgct gtcagccagg ctggtgacaa cgaagacgcg tccgccgcgt ccgccgacgg
     9061 tgtacatgcc accgccctcg gcgccgggga acgcgggaat ctccgcctgc ggcagatcgt
     9121 ccggattcag cgccagcgcg atgtacggtt tgccgttggc ggcccagcgc gccacatccg
     9181 gctccgcgcg atgccacgcg tcgtcatcga gccgcttcca ctcgcgacgc cgcgcctcgt
     9241 ccgccgcctt gatctccggc ggaattgccg gatagccctt cttatattgc acctgccccg
     9301 gcggcagcgt gtaaaccggc tgtgatgtct gcccgcgcgc gttcgtcgca acggcgagcg
     9361 cgagaaataa tgcgatgcag taggcgaatg atttcataga tatgtagcac gggcgtctgg
     9421 cccgtgccta ggtgctcgat acgggcgaga cgcccgtgct acggaaattc atttgactga
     9481 tgccgtcagc tgcgccggcg ccagcgtgtt cacgttgttc ttcagatcgt gccaatcgac
     9541 cttctgcgtc ggttccaggc cgttcaggta tttttcgatg tttgtgtacc cgtcgccgtt
     9601 gcagtccttg ccggccaggt ccgggtcgtt cacatccagg tgatatttct gcttccacca
     9661 gtcggggatg ccatcgttct gcgtgtactt cacgggctcg cctttgtatt ccggatatcc
     9721 gccgacctga ttgacgtcgg tgatgatgcc gttgccggcc acgccgatgt tgttcttcgc
     9781 caggtccttc atgggctcga tcggaatctc cttgcccatg ccccaggtca cgccagtgcg
     9841 gacttcttcg atcacccgct ggtccaccgc atctcgccgg ggcagtgtcg cgccagcatt
     9901 agccagcacc gactggaacg catcttccgc cgattgaatc gtcatcggcg gcatcgggaa
     9961 gggtttgtct tcgcgcacct tcgcgatgat ctccttgagt ttgtcgtcat cgcggatcgc
    10021 gccgttggcg atcgcgccgc tggcatcccg atccggcgca aactggaact gcaccccgcc
    10081 gtcccagttg tttttggtca ccttctcatt gccttcgaca acattgccgg cggcgtaaac
    10141 tttgccccat cggctgaccg ggttcgcctt cgaccaactc tgcgccggtt cgacaatgcg
    10201 atatcgcacc ggtgagtccg gcgtggccgg gccaggcttg taatagttgt tgatcatgtt
    10261 gatctggctg tgctcatcgc cgccgtccac cgatcgatgc cgccagttga acagcacgtt
    10321 gttgacgaag ttgaagtcgt acgacatcgc gatgcttgga tttcggccgg tgttgcaggc
    10381 gaacaggtcg tg
//
LOCUS       DATIMH010000331         5599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5559999, whole genome shotgun sequence.
ACCESSION   DATIMH010000331 DATIMH010000000
VERSION     DATIMH010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5559999"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(855..1160)
                     /locus_tag="VLI90_05665"
     CDS             complement(855..1160)
                     /locus_tag="VLI90_05665"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV13726.1"
                     /translation="MKRHSLQAIDKLLGPSRVAAAKANARGKMEAMLLSEVRKQLGFT
                     QTAVAKAMGVSQSALSQMESQDDLQLSTLRRLVSALGGELDIVARFGDRAIVLVDAK"
     gene            complement(1157..1540)
                     /locus_tag="VLI90_05670"
     CDS             complement(1157..1540)
                     /locus_tag="VLI90_05670"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSV13727.1"
                     /translation="MPVSAPGQRWNIEITDDYLQWFRALDEGQQDALRTDIEILEQMG
                     PALGRPQVDSIKGSRHSNMKELRTMHARRQVRSFFAFDPRRTAILLIGGDKTGKRRFY
                     ERMVPLADRLFDQYLADIRKEGLIP"
     gene            1921..3021
                     /locus_tag="VLI90_05675"
     CDS             1921..3021
                     /locus_tag="VLI90_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13728.1"
                     /translation="MLRKTNRITKTTKTNTSAAQPMFERLEDRRLQSATPINYDGQYV
                     NYTNNVRYEVDFKHSTSATKYTGDVKSGKQDLKLTATETNNKLAGSINVNGKSSNFTA
                     VLNGRQLTLTMGSTKVIYTKRVQTLTPHTASSLFEYDTPSGWTARANTGGLLIKSADG
                     TQQVSVVSGVSSGWIAPQALVNNAAQHGTHVKLTSSSPVYNLGGGLYLADEVAEVTFN
                     VGKTNFTSDVIIASVYNASYAITKVGMASITAPTSVFAKNGGVLVDILSSVHQINTGT
                     GAAAASPAAEVAGGMVASPAVAAGNIWSSFDYGYTFTNGFYDPGVVGNWAYGYTTAWV
                     SPFVAAEQVYMAQTSSAFDAEVAAWHEQFNAT"
     gene            complement(3228..4133)
                     /locus_tag="VLI90_05680"
     CDS             complement(3228..4133)
                     /locus_tag="VLI90_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSV13729.1"
                     /translation="MAQPHLLNHRLLGANIFLPGHAPVRATRTIINGATPMDDRDCMD
                     IFLVVSGTGTHRTIYGDQTLARGDAYILRPGAWHSYQNAKSLELLQCRFGLELLQHEL
                     AWTRNDPAMELMFWAAPLSLDRRGLLHVPLTGATLDRCQALLEELVVLTSPGSAGLRA
                     EEIGRLLLFLSEMGREVAASLLKLRRGKPEPHQAVTEGIRLMEEDIRRDWTLPELAKR
                     LKLDKSYLVRLFRAHTGSPPMQFLTRLRAERAAMLLLRTQRDIGIIGGQVGWNDPNYF
                     ARRFRAHFGVSATNYRRQFAEELSA"
     gene            4344..4646
                     /locus_tag="VLI90_05685"
     CDS             4344..4646
                     /locus_tag="VLI90_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSV13730.1"
                     /translation="MAGPSSITSIPNRRRGRRHRLDIPASLAPTGGDPGRPGIAVRIS
                     ELSVAGVGLRSDQPLEPGHVYEVRAFDTLVPTGMRVKVISHRAAADGGFEIGAESL"
     gene            4880..>5599
                     /locus_tag="VLI90_05690"
     CDS             4880..>5599
                     /locus_tag="VLI90_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13731.1"
                     /translation="MQTVAHVTHEAIQKIGGIGAVLQGLLTSRTYLDKAQRNILVGPF
                     WPSDGAGEQRLGPAGEVLYSSMDSLYRSPLAARFREIEQTFDVGIVYGRRKFSDKETG
                     VTSVPEVLLIDVSRYDAGKIGTFKFQLWEKFGIDSSKYENIWDYEQYMRLAQPAVAAL
                     HALGATSSVEPCVILSHEYMGMPTALAAILEDGSKGNFRTIFYAHEVATMRRIVEGHG
                     GHDTMFYNVMRSALASGHFVED"
BASE COUNT         1139 a         1739 c         1693 g         1028 t
ORIGIN      
        1 ggagttatgt aaatcctttc gcggggagtg cccacttccg gattcctgcg cttcatgtag
       61 gagatctggc aggtaacgaa ggcccggtat gggtttggcc ccctcccaca tttggcggaa
      121 agcatcatgt accatgatcc gcttctgaac tcgttgaagc gtcgaataaa gtggaacacc
      181 tcgaatagag atcgctaacc gtagcgactc gcatgccgcc agtttcgata tcacatcgcg
      241 gtaactcgca cccgttagtc tccgaagcat caccagcgcc gctagttgcg gaacagtaaa
      301 gtcgcggcgg ctttcagagc aggaatacgt tggcaagatt ttatggctca ccgagtagca
      361 ggctcgaaga agctcgtaaa ggggttccga cggcataggt tgcgcgggat ttcaacagca
      421 tttcaacagc atttcaagaa gcgccgttcc gcagacgctc ccagcgatta ggagttctag
      481 cctccgtcga ggcgaacagc ccggtccact tgccggagaa acgatatgaa agcagctgaa
      541 gcgatcaccc ttgagttggg ggaaccaaaa tacggcacga acaagatgct ccgctggaaa
      601 agcacgccag ggatggccgt gaatgtacag gtcgacaccc aacggtggaa catttggctg
      661 ccgtggacag agactctcct agtcccacat ttcgcaaccg tgcaccaaag cggcggcaag
      721 cattccaatc tttcgccaga gttgaagcac ggcatcgtcg tgcgacttca gccggagacg
      781 aacgctgatc gagcggcggc ggtgcagttg gttttgaggt tgtggcaaag cggggtaagc
      841 cgctgtgaac gtagctactt cgcatccacc agcacaatcg cccgatcgcc aaaccgggcg
      901 acgatgtcga gttccccgcc cagcgcgctg accagtcgcc gcagcgtgct gagttgaaga
      961 tcgtcctgcg attccatctg cgacagcgcc gactggctca cgcccattgc cttggcgacg
     1021 gcggtttgag tgaagcccaa ctgcttgcgc acttcgctca gcagcatcgc ttccatcttc
     1081 ccccgggcat tggccttcgc cgccgcaacg cggctagggc ccaggagctt gtcgatcgcc
     1141 tgcagggaat gtcgcttcat ggaatcaacc cttcctttcg aatatcggcc agatactgat
     1201 cgaacagccg atctgccagc ggcaccattc gttcataaaa cctccgtttt ccggtcttgt
     1261 cgccgccgat gaggagaatc gccgttcgcc gcggatcgaa tgcgaagaag ctgcgaactt
     1321 gtcgccgcgc gtgcatcgtg cgcagctcct tcatgtttga atgcctagag cctttgatgg
     1381 aatccacctg cggacgcccg agcgcgggac ccatctgctc gaggatctcg atatccgtac
     1441 gcagcgcatc ctgctgtcct tcgtccaacg ccctgaacca ctgcaaatag tcatcagtga
     1501 tttcgatatt ccatcgctga cccggggccg acaccggcac cgtataagat ttggctaatg
     1561 ataatcaacg cacaaaggcc atcgccggag gtaactaaaa caagttcttc acgcggagat
     1621 tctggagacg gacaaacggc gcaggagttt gaccctccac gtctttgcgt tcatccactc
     1681 attccccgtt cgctcaacct attatgcgcg gcaatgattg ctctaatcaa cgcacgtcca
     1741 taagtcgcaa atccttttta cgccgcttga tacagttgac gctttcccac cgcggggcag
     1801 gctgcgcagg ctccgccgcc cgccgcagta cccacttgtg tcatcgagga accggcggct
     1861 gagtcagccc cggagatgac gtgaaacgaa gcctgcaaag agctgattca ggagacggta
     1921 atgttgcgaa agaccaacag gatcacgaag acgaccaaga ccaacaccag cgccgctcag
     1981 ccgatgttcg agcggttgga agaccgccgg ttgcagtcgg ccacgccgat caactacgac
     2041 gggcagtacg tcaattacac caacaacgtc cgttatgaag ttgacttcaa gcacagcacc
     2101 agcgcgacca agtacaccgg cgacgtcaag agcggcaagc aggacctgaa gctgaccgcg
     2161 accgagacga acaacaagct ggccgggtcg atcaacgtca acggcaagtc gagcaacttc
     2221 accgccgtgt tgaacggccg acagctgacc ttgaccatgg gaagcaccaa ggtgatctac
     2281 accaagcgcg tgcagacgct gacgccgcac acggcatcga gcttgttcga atacgacacg
     2341 cccagcggtt ggaccgccag ggcgaacacc ggcgggttgc tgatcaagag cgccgacggg
     2401 acgcagcagg tgtcggtggt cagcggtgtc agctccggtt ggatcgcgcc gcaggcgctg
     2461 gtcaacaacg cggcccagca tggcacgcac gtgaagctca cgtcgtcgtc gccggtgtac
     2521 aacctgggcg gcgggcttta cctggccgac gaagtggccg aggtgacgtt caacgtcggc
     2581 aagaccaact tcaccagcga cgtgatcatc gcgtcggtct acaacgccag ctacgcgatc
     2641 accaaggtcg gcatggccag catcaccgcg ccgacgagcg tgttcgccaa gaacggcggc
     2701 gtgctggtgg acatcctcag cagcgtgcac cagatcaaca ccggcaccgg cgcggccgct
     2761 gcaagccccg cggcggaggt cgccggtggc atggtcgcca gccccgcggt tgccgcgggc
     2821 aacatctggt cgtcgttcga ctatggctac acgttcacca acggcttcta cgaccccggc
     2881 gtcgtcggca actgggcgta cggttacacc accgcgtggg tcagtccctt cgtggccgcc
     2941 gagcaggtgt acatggcgca gacgagcagc gcgtttgatg cggaagtcgc cgcgtggcat
     3001 gagcagttca atgcgaccta atttgtcatc ctgagcgcag gcgtacaggc cggaggcgaa
     3061 ggatctgctc gagaagtgat agaacgatcc gatagcacag gcgttcatca acgttcgaag
     3121 acccgcatgt acatgcgggc tccggcgcgg atggacgcct ctcccggagt accgggcgag
     3181 gggaccagag gagattatgc gcgagcgcgc atgccgttgt gtgcgaatta cgcgctcagc
     3241 tcttccgcga attgccggcg gtagttcgtc gcgctcacgc cgaagtgggc gcggaagcgc
     3301 cgggcgaaat aattcggatc gttccacccc acctgtccgc caatgatgcc gatgtcgcgc
     3361 tgcgtgcgca gcagcagcat cgcggcccgc tcggcgcgca gccgggtgag gaactgcatc
     3421 ggcggcgagc cggtgtgggc gcggaacaat cgcacgagat atgacttgtc cagcttcaga
     3481 cgcttggcga gctccggcag cgtccagtct cggcggatgt cttcttccat caggcggatg
     3541 ccctcggtca cggcctggtg cggctcgggc ttgccgcggc gaagtttcag caacgacgcc
     3601 gccacttcgc ggcccatctc agacaggaat agcagcagcc ggccgatttc ttcagcgcgc
     3661 aaccccgccg atcccggcga cgtcagcacg accagttctt cgagcaacgc ctgacatcga
     3721 tccagcgtcg cgccggtcag cgggacatgc agcaacccgc ggcggtcgag cgatagcggc
     3781 gccgcccaga acatcagctc catcgccggg tcgttgcgcg tccacgccaa ctcgtgctgc
     3841 agcagttcca agccgaaccg gcactgaagc aattccagcg actttgcatt ctgatacgag
     3901 tgccacgccc cgggacgaag gatgtacgcg tcaccgcgcg caagcgtctg gtcgccatag
     3961 atcgtgcggt gagtgccggt gccgctgacg accagaaaga tgtccataca atcgcggtcg
     4021 tccatcggcg tggcgccgtt gatgatggtg cgcgtcgccc gcacgggcgc gtgcccgggc
     4081 aggaaaatgt tcgccccgag cagtcgatgg ttcagcagat gcggttgggc cacgcctatg
     4141 gtttcgcaga ctgttgcctt gatgtcaata ttgtcctatt ttgcaaacac gttcttcacg
     4201 cgtccccgcg acgatctggg caacattccg ggccaatacc ggccagctgc gcacgcctgc
     4261 gctcgaaaac ccgccgttat cagactgata cgaaccggcg cagcgcgctt gagtgcgtcg
     4321 cggatggcgt cgatccaaag gacatggcag gcccgtcatc gatcacctcg atccccaatc
     4381 gccgccgtgg ccggcggcat cgactggata tccccgcgtc gctcgcgccc accgggggtg
     4441 acccgggccg tccgggcatt gcggtgcgca tttcggaatt gtcggtcgcg ggcgtggggt
     4501 tgcggtcgga tcagccgctg gaaccggggc atgtttacga ggtgcgcgca tttgacacgc
     4561 tggtgccgac aggcatgcgc gtcaaggtca tctcgcatcg cgccgccgcc gatgggggtt
     4621 ttgaaatcgg cgccgaatcg ctttgattgc gaccccgcac ccgcggcatg ccgatagctt
     4681 gtacggcgga tgctgcgcgc tcgctgcgcg tttgttggca attcccgcgc gtgcgcgtgg
     4741 ctgttgcagg gaatggatgc cgctgctttc gccgggcaag tggctcctct atacttctcg
     4801 attcggccgt tcgcgaacac gcgcgcggcc gcgcgggtga acctcacata cttccggctg
     4861 acatcgaagg atttgttcta tgcagactgt cgctcacgtt acacacgaag ccattcaaaa
     4921 aatcggtggc attggcgccg tccttcaggg cctgctcacc agccgcacgt atctcgataa
     4981 agcgcagcgc aacatcctgg ttggcccatt ctggcccagc gacggcgcgg gcgaacagcg
     5041 cctcggtccc gcgggggaag tgctgtacag cagcatggac tcgctctatc gctcgccgct
     5101 ggccgctcgc tttcgcgaaa tcgagcagac gttcgacgtc ggcatcgtct atggccggcg
     5161 gaaattcagc gacaaggaaa ccggcgtcac cagcgtgccg gaagttttgt taatcgacgt
     5221 ctcgcgctac gacgcgggca agatcggcac gttcaaattt cagctgtggg aaaagttcgg
     5281 catcgactcg agcaagtacg aaaacatctg ggattacgag cagtacatgc ggctggcgca
     5341 gccggccgtc gcggcgctgc acgcgctggg cgcgacgagc agcgtcgagc cttgcgtgat
     5401 cctctcgcac gagtacatgg gcatgccgac ggcgctggcc gcgatcctgg aagacggcag
     5461 caagggcaac ttccgcacga tcttctatgc ccacgaggtc gccacgatgc gccgcatcgt
     5521 cgaagggcac ggcgggcacg acacgatgtt ctacaacgtc atgcgcagcg cgctggcgtc
     5581 cgggcatttc gtcgaagac
//
LOCUS       DATIMH010000332         4073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1230022, whole genome shotgun sequence.
ACCESSION   DATIMH010000332 DATIMH010000000
VERSION     DATIMH010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230022"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..697
                     /gene="ybeY"
                     /locus_tag="VLI90_05695"
     CDS             182..697
                     /gene="ybeY"
                     /locus_tag="VLI90_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HSV13732.1"
                     /translation="MTPTTRRQSVPALQLSIRADVGALHVPYLRRHIRKAHELLAPPL
                     RELSIALVNDRRMSDLHVRFMNIPGPTDVLTFPLELLKNGKATAGEVILCVPEARRRA
                     KEHRVTVQNELLLYALHGMLHLCGLDDRTARGFARMHRTEDDILQRLGVGRVFHSRRA
                     SQTSGRTRGGR"
     gene            697..1983
                     /locus_tag="VLI90_05700"
     CDS             697..1983
                     /locus_tag="VLI90_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSV13733.1"
                     /translation="MTYYFIIAGIGLASIASLFFSTLTYSLRDFARPRLQEQMERRGN
                     ETLFDRIADHAADLIFVTAIGRLFANILVLIFLLRLFNETHHALWLQYLYGVVVTAII
                     TLFVSVAIPNAAAKYASEACIATCAGFLNGARVAMSPVTKIMHVIDDVIRRAAGAHDT
                     PQPEQLEEEILSVVEEGEKEGIVDVQEREMIESVIAFRDTTASQIMTNRPEIIAIEAP
                     VRIDQVKDLVERSGHSRIPVYQDNIDHIIGILFARDLLKYIGLPLEKFEIRPHLKTPL
                     FVPESKPLRDLLKDFRLQKIHLAIVQDEYGGTAGLVTIEDILEELVGDIADEHERSEP
                     AMLQRINDQTWEADARVYIDELNRAIGLSIPDDVGFDTLGGFLTTTMGRIPPAGATLD
                     HEGVKYTVLDAEPQRVNRVRIELVPEPATEAPASRG"
     gene            2088..2303
                     /locus_tag="VLI90_05705"
     CDS             2088..2303
                     /locus_tag="VLI90_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13734.1"
                     /translation="MRTTLAVILLTSALLPVAGGCEQALETGYIPRKLNASESERRAF
                     YAPEYTPEANGSKDSPAAAPNLGMTHR"
     gene            2464..3123
                     /locus_tag="VLI90_05710"
     CDS             2464..3123
                     /locus_tag="VLI90_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV13735.1"
                     /translation="MTAAIDPVQFVSSVRPLLERQDLQGLVGLLEERWTCDQIAHLLM
                     TGDEDTRKVAALCLSLVGKRCTIDKIAEQLKDPDPMVNQMAEHALWSIWFRCGDPASN
                     KELCRGTKALNAREFDRAIEHFDRAIEHDDNFAEAYNQRAIAHYLQEQYESSVRDCRT
                     AVERMPCHFGAWAGMGHCFLHMGELAKALQCYEQALSINPHLDCLRETIKEIKGRLEE
                     E"
     gene            complement(3351..3423)
                     /locus_tag="VLI90_05715"
     tRNA            complement(3351..3423)
                     /locus_tag="VLI90_05715"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3387..3389),aa:Glu,seq:ctc)
     gene            3638..3708
                     /locus_tag="VLI90_05720"
     tRNA            3638..3708
                     /locus_tag="VLI90_05720"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3670..3672,aa:Ala,seq:cgc)
     gene            3806..>4073
                     /locus_tag="VLI90_05725"
     CDS             3806..>4073
                     /locus_tag="VLI90_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HSV13736.1"
                     /translation="MSASAPADSFFVYIVRCVDDSFYVGHTSDLKERVNTHNEGRGAS
                     WIACRRPVQLVYHERHDSEEKAVARERQIKRWTHDKSWPSSKETE"
BASE COUNT          823 a         1292 c         1197 g          761 t
ORIGIN      
        1 ctcgggcact gacccagagt ttggcgttac cggattgaag gtcgactccc tcatccgctt
       61 ggacaaactg attactttgt cgaccggttt gattgcgcgg cgactggtcg ctaccggacc
      121 gggtgcccga gcgcgcgtcg cttcgatgct ccggcgcgca ttcgacttga agtaaacaat
      181 catgacgccg accacacgcc gccagtctgt gccggcactt cagctatcga tccgggccga
      241 cgtcggagca ttgcacgtgc cgtatctccg ccggcacatt cgcaaagccc acgaactgct
      301 cgcaccaccg cttcgcgagt tgtcgatcgc actcgtcaac gaccgccgca tgagcgactt
      361 gcacgtgcgc ttcatgaaca tccccggccc aaccgacgtg ctgacgtttc cgctcgaact
      421 gctgaagaac ggcaaagcga ccgccggcga ggtcatcctt tgcgtgccgg aggcccgccg
      481 gcgcgcgaaa gaacaccgcg taaccgtgca aaatgaactc cttttgtacg cccttcacgg
      541 catgttgcac ttgtgcggtt tggacgatag aaccgcccgc gggttcgcac gcatgcaccg
      601 caccgaagac gacattctgc agcgcctcgg cgtcgggcgg gtcttccaca gccgccgcgc
      661 cagccaaacc tccggccgta cccgcggagg ccgctgatga cgtattactt catcatcgcc
      721 ggcatcggcc tggcatcgat cgcgtcgctg ttcttttcca cgctgacgta ttcgctgcgc
      781 gacttcgcgc gcccgcggtt gcaggaacag atggagcgcc gcggcaacga gacgctcttc
      841 gaccgcatcg ccgatcacgc cgccgatctc attttcgtta ccgccatcgg ccggttgttc
      901 gcgaacatcc tcgtgctgat cttcctgctg cggctcttca acgaaacgca ccacgcgctg
      961 tggctgcagt atctctacgg agtcgttgtc actgcgatta tcacgctatt cgtatccgtg
     1021 gcgatcccca acgccgccgc caagtatgcc agtgaagcgt gcatcgcgac gtgcgcgggt
     1081 tttttaaacg gcgctcgcgt ggcgatgtcg ccggtgacca agatcatgca cgtgatcgac
     1141 gacgtgatcc gccgcgccgc cggcgctcac gacacgccgc aaccggagca gctcgaggaa
     1201 gagattttgt cggtggtcga agagggggag aaggaaggga tcgttgacgt gcaggagcgc
     1261 gagatgatcg agtcggtcat cgccttccgc gacaccaccg ccagccagat catgaccaat
     1321 cgcccggaga tcatcgcgat cgaagcgccc gtccgcatcg accaggtgaa agacctggtc
     1381 gagcgcagcg gccattcgcg gatcccggtt tatcaggaca acatcgatca catcatcggc
     1441 atcctgttcg cgcgcgatct gctgaaatat atcggcttgc cactggaaaa attcgagatc
     1501 cgcccgcacc tcaagacgcc gctgttcgtg ccggagtcca aaccgctgcg cgacctgctg
     1561 aaggattttc gcctgcagaa gatccacctg gcgatcgtgc aggacgaata cggcggcacc
     1621 gccgggctgg tcacgatcga agacattttg gaagaactgg tcggcgacat cgccgacgaa
     1681 cacgagcgct ccgagccggc gatgctgcag cgcatcaacg accaaacgtg ggaggccgac
     1741 gcccgcgtgt atatcgacga gctgaatcgc gcgatcggcc tgtcgatccc cgatgacgtc
     1801 ggcttcgaca cgctcggcgg gtttttgacg acgacgatgg gccgcatccc accagccggc
     1861 gcgacgctcg atcacgaagg cgtgaagtac acggtgctgg acgccgaacc gcaacgcgtg
     1921 aaccgcgtgc ggatcgagct ggtgcccgag ccggccaccg aagccccggc gagccgcggg
     1981 taatgtgatg ataacaatca cgccggattc ggagtacctc cgtacccggc gtgatgtgta
     2041 tcagcgcata tcgcattgcc ccaacatcaa tgttatcgtt gcccgccatg cgaacgacgc
     2101 tcgctgtaat tctcctcaca tcggcactgc tgcccgtcgc cggcgggtgt gaacaggcgc
     2161 tggaaactgg ctacatcccg cggaagttga acgccagcga atccgagcgg cgggcgtttt
     2221 acgccccgga gtacacgccg gaggcgaacg gttcgaagga cagccccgcg gccgcgccca
     2281 acctgggcat gacgcatcga tgatcgcatc ggtcgaggtc ggcaatatcc ggttttccgt
     2341 ggaaaccggc acctccgtta cctacacttg agccacgggg aacacatttt cgccgtctcc
     2401 ccccgtgtga tatgctcatt ttcagccgaa ccattgctgt atccaacaca aggcgatgac
     2461 ggcatgactg ctgcgatcga tccagttcag ttcgtctcgt cggtccgtcc gctcctggag
     2521 cggcaggacc tgcaggggtt ggtcgggctg ctggaagaac gctggacctg cgaccagatc
     2581 gctcacctcc tcatgaccgg tgacgaggac acccgcaaag tggcggcgtt gtgcctgtca
     2641 ctcgtcggca agcgctgcac gatcgataag attgccgagc agctgaaaga ccccgatcca
     2701 atggtgaacc agatggccga gcacgcgctt tggtcgatct ggttccgctg cggtgacccc
     2761 gcgtccaaca aagaactgtg ccgcggcacc aaggcgctga acgcgcgcga atttgaccgg
     2821 gccatcgaac acttcgatcg cgcgatcgag cacgacgaca acttcgccga ggcatacaac
     2881 cagcgggcga tcgcgcatta cctgcaggag cagtacgagt catcggtgcg cgattgccgc
     2941 accgcggtcg agcgcatgcc atgccacttc ggcgcctggg ccggaatggg ccactgcttc
     3001 ctgcacatgg gcgaactggc gaaggcgctg caatgctacg agcaggcgct gagcatcaat
     3061 ccccacctcg attgtctgcg ggagacgatc aaagaaatca aggggcgctt ggaagaggag
     3121 taggcgcttt cgtctacttt cgctgtctcc tgcgtagcgg tcgccccgaa ggggcatgcg
     3181 tttttcctct aagagacaaa agaaatcccc cagttgtggt cagcatctaa caatcgcctc
     3241 cgctcggata gttcggcctc ggcgtggcac tcctcgccga tccattcgta atagttgccg
     3301 gataatggcg gactatgcgc aataaaaaac ccgcctttcg gcgggtccag tacccccaag
     3361 gggagtcgaa cccctgtctt ctggctgaga accagacatc ctaggccact agacgatggg
     3421 ggcgtctgcg gatcgttggc gcgagcagtg cagctgctga gcctaggcga aggcattatt
     3481 caaccgcggc agtggcgcgt caagcagcgg ccgttttgcg cgccacaacc gtgccacgcg
     3541 cggccgcagt gcttgacgac cgggcgtttg cacctaaaat gaccaccccg cacgcgtgac
     3601 gcagcagcag cggcgagtcg tgcaactgaa atatagcggg gccatggtct aacgggatga
     3661 cggctgaatc gcattcagca aatcggggtt cgattccccg tggctccatt cgactcggcc
     3721 gggtacggcc tcgctcatgg caggccagct ccggccgagt cgaatgccct gagcgaacgt
     3781 actcgtgagt cgaacgggct tttccatgtc cgcatctgcg ccagcagatt cctttttcgt
     3841 ctacatcgtt cgttgcgttg atgacagttt ttacgttgga catacatccg accttaaaga
     3901 acgagtcaat acccataatg aaggccgcgg tgcttcgtgg attgcctgtc gccgtccagt
     3961 ccagctggtc taccacgaac gacatgactc tgaagaaaaa gcggttgccc gcgaacgcca
     4021 gatcaagcgc tggacacatg acaaaagctg gccctcatca aaggagaccg agc
//
LOCUS       DATIMH010000333         4214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4403911, whole genome shotgun sequence.
ACCESSION   DATIMH010000333 DATIMH010000000
VERSION     DATIMH010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4403911"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="VLI90_05730"
     CDS             complement(<1..1471)
                     /locus_tag="VLI90_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13737.1"
                     /translation="MQFLCSGIYLLLITATGALAWTWVNAKRVHLIGAAGFAFAAGSG
                     LLGLLLFDLSLCGVAPSRPELSAIALIVAIGLILRRRALPRLTFPRAMPKRWDGWTWL
                     GAIALLVLIAAVVNVAIADVTFGLSDIDAFGIWMLKAKAAYLAPLRPIPAVMTHPSFS
                     YSHQDYPLGFPMVIAGFYAMVGGVDEQLAKTALLPPIYASLIAVMYTSLRWLLPARRA
                     PAICITAIFAAAPVLTTHAGMAVAELSLVLMHACCLALLMRWMIEPDRRLLIASAVFA
                     ALAAMEKNEGLALLPIVALVPFGFAVSRRVRRYSDDPDSAAGRAEESRSSRIPFDKLR
                     AGCSEPGVLWDWIIAWLTAGALIAPWLIYRCYLPRTHEDYGSKLTSITTLLHDLPRLG
                     HVLPRYLRLFVAFSSAGAIWILLLIVAICCHKTFRRAVVRAMWILLLLHAGLYMLTFV
                     VTPWDVDVLLPMVGPKLLMHIAPTAVLLIGLHLSNSCEDG"
     gene            complement(1524..2003)
                     /locus_tag="VLI90_05735"
     CDS             complement(1524..2003)
                     /locus_tag="VLI90_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13738.1"
                     /translation="MSEDGPSKSARSKLLYACVLIALLQALYENVQSNAPLQQLRSGS
                     SPQLAVEQRLSGMDPLVATAADGGNLAMHFDGFDPAEFHQGLLMSMLYFRAAYALYPH
                     RVLVGPDGRVINSATDLAAASSTLPDDNWLKRHDVRAVMDIHQIAGGGVYQQVRAIP"
     gene            2068..2715
                     /locus_tag="VLI90_05740"
     CDS             2068..2715
                     /locus_tag="VLI90_05740"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HSV13739.1"
                     /translation="MNGTIRRVHDFELDGRGSASAWEAVQWWAIEPLKSAGDGYRTRV
                     KLAYSGTGIYCLYDCDDRVLTSSGLGDNQDLWKEDVVEAFLWTDERHPVYFEYELSPL
                     GAELPLLVPNDRGTFIGWVPWHYGEARRTRRATNIRGGERAAGAAISGWTAEFFIPFA
                     LLRGLSNVPPTPGATWRANFYRIDHDAGEPALFAWSGGVGDSFHNINEFGTLLFE"
     gene            2726..3091
                     /locus_tag="VLI90_05745"
     CDS             2726..3091
                     /locus_tag="VLI90_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSV13740.1"
                     /translation="MPKLAIDGGAAVDVPPGKRLVNALTDEGGIDQLHACGGFARCTT
                     CRVQFVAGEPSAMTQAERDVLTSRALSGQGGLRLSCQIVCDHDIEVRAISRLAGSGRV
                     DAGKRPADQIEPPAVWVSK"
     gene            complement(3139..>4214)
                     /locus_tag="VLI90_05750"
     CDS             complement(3139..>4214)
                     /locus_tag="VLI90_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13741.1"
                     /translation="LFVPSRVISEDKSMPTGTGSTFVCSACGKKFVWKPELAGKTVRC
                     KCGQSIAVPTGAAPAVAAPAAARPAVKSQLAPQTAAVRKPPKPAASNEPSLDDLYDLA
                     ADADSAPASRSSAAMESAAVAAPVAVPGLEDDAYRCPSCNEPMQAGSILCGSCGFNLK
                     TGEKMTVKRAGGTVARPAAGGGGGGSPSGSAFSGIPARSKPKFVEDKQGQMMKILIPV
                     FLIAAVIGGIFAFKIIAGRSGNSAAALSTGKGDDPDVAEKMENENPMEVHAWFKQNPS
                     RMMGELSERQAIGKADELEKMGAKKCYAFGSMMCLCMAVELPDDPAQRKALIDYRNKW
                     NVERHYKPVKDEGQKYMILNFGL"
BASE COUNT          767 a         1269 c         1438 g          740 t
ORIGIN      
        1 gtccatcctc gcagctgttc gataggtgaa gtccgatcaa caatacagcc gtcggcgcga
       61 tgtgcatcaa cagctttggc cccaccatcg gcagcagcac atcgacatcc cacggcgtga
      121 cgacgaacgt taacatgtat aaccccgcgt gcagcagcag caggatccac atcgcgcgaa
      181 caaccgcccg gcggaacgtc ttgtggcagc agatcgcgac gatgagcagc aatatccaga
      241 ttgcgccggc cgaactgaac gccacgaaaa gccgcaagta tcgcggcagc acgtgcccca
      301 gccgtggcag atcgtgaaga agcgtggtga tgcttgtgag cttgctgccg taatcttcgt
      361 gcgtgcgcgg gaggtaacag cggtagatga gccagggcgc gatgagtgcg ccggcggtga
      421 gccaagcgat gatccagtcc cacaacacgc cgggttcgga gcagcctgcc ctgagcttgt
      481 cgaagggtat tcgcgacgac ctggattctt ctgctcgacc cgccgcagaa tccgggtcgt
      541 cggagtacct ccgaacccgg cgtgaaacgg caaatccaaa aggaaccagc gctacaatcg
      601 gcagcagcgc gagcccctcg ttcttttcca tcgccgccaa cgccgcgaag accgcgctcg
      661 cgatcagcag ccggcggtct ggttcgatca tccatcgcat cagcagcgcc agacagcaag
      721 cgtgcatcag caccagcgag agttccgcga ccgccatccc cgcgtgcgtg gtcaacaccg
      781 gcgcggcggc gaagatcgcg gtgatgcaga ttgccggcgc ccgccgggcc ggcagcaacc
      841 atcgaagcga tgtgtacatc accgcgatga gcgatgcgta aatcggcggc agcagcgccg
      901 tcttcgccag ttgctcatcg acgccgccga ccatcgcgta aaagccggcg atgaccatcg
      961 ggaaacccag tgggtaatcc tgatggctgt agctgaacga cgggtgggtc atcaccgccg
     1021 ggatcggccg cagcggggcg aggtacgcgg ccttcgcttt caacatccag atgccgaagg
     1081 catcaatatc agagaggccg aaggtgacgt ccgcgatcgc cacattgacc accgcggcga
     1141 ttagcacgag cagcgcgatg gccccaagcc acgtccagcc gtcccatcgt ttgggcatcg
     1201 cccgcggaaa cgtgaggcgc ggcagcgcgc ggcgccgcag gatcaaacca atagcgacga
     1261 tgagcgcaat ggcgctgagt tctggccggc tcggggcgac gccgcagagg gaaagatcga
     1321 acagcagcag gcccagtagt ccagaccccg cggcgaatgc gaagccggcg gcgccgatga
     1381 gatggactcg ctttgcgttg acccacgtcc aagcgagggc tccggtcgcc gtaatgagca
     1441 ggaggtagat gccggagcag agaaattgca tgagcctcac cccctcttcc acgcgtgagt
     1501 ggacgagggg aacgcggagc agatcatggg atcgcccgca cctgttgata cacgccgccg
     1561 ccggcgatct ggtggatgtc cattacggcg cgaacgtcat ggcgcttgag ccaattgtca
     1621 tctggcaggg tcgatgaagc ggccgcgaga tcggttgcgg agttaatcac ccggccatcg
     1681 gggccgacca gcacgcggtg gggatacagc gcgtacgccg cgcgaaaata caacatgctc
     1741 atcaacaggc cctgatgaaa ttcggcggga tcgaagccgt cgaagtgcat cgccaggttt
     1801 ccgccgtcgg cggcggtggc gaccaacggg tccatgccgc tcagccgttg ctcgaccgcc
     1861 agctgcggtg aggagccgct gcgcagctgc tgcagcggcg cgttggattg cacgttctcg
     1921 tacaacgcct gcaagagcgc gatcagcacg cacgcgtaca acagcttcga tcgtgcggat
     1981 ttgctgggcc cgtcctcgct cacgcggcga atcttaccgg cgaggttcgg cttgtgtaat
     2041 ggaaggccat cgctacgctc acgcgcgatg aacgggacga tcaggcgcgt gcacgatttc
     2101 gagctggatg gccgcgggtc ggccagcgcg tgggaggccg tgcagtggtg ggcgattgag
     2161 ccgttaaagt ccgctggcga tggttatcgc acgcgcgtga agctcgccta ctcgggcacc
     2221 ggcatctact gcttgtacga ctgcgatgat cgcgtgctca cgtcgagcgg attgggcgac
     2281 aatcaggacc tgtggaagga agacgtcgtc gaggcattct tatggacgga cgagcggcat
     2341 ccggtttatt tcgagtatga gctgtcgccg ctcggggcgg agttgccgct gctggtgccg
     2401 aacgatcgcg ggacgttcat cggttgggtg ccgtggcact acggcgaggc gcggcggacg
     2461 cgacgcgcga caaacatccg cggcggtgag cgcgcggcgg gggcggctat cagcggatgg
     2521 acggcggagt ttttcattcc gttcgcgctg ctgcgcggat tgagcaatgt cccaccgacg
     2581 ccgggcgcga cgtggcgggc gaacttttat cgcatcgatc atgacgccgg cgagcccgcg
     2641 ctgttcgcgt ggtccggcgg ggtgggcgat tcatttcaca atatcaatga gtttggaacg
     2701 ctgctgtttg aatgatgaga acgctatgcc gaagctggcg atcgatggcg gtgcggcggt
     2761 ggatgtgccg ccgggtaagc ggttggtgaa cgcgctgacg gacgagggtg ggatcgacca
     2821 attgcacgcg tgcggcgggt tcgcgcgatg cacgacgtgc cgggtgcagt tcgtcgcggg
     2881 cgagcccagc gcgatgacgc aggcggagcg ggatgtgctg acgtcgcgcg cgctgagcgg
     2941 gcaaggcggg ttgcggttga gctgccagat tgtttgcgat cacgacatcg aggtgcgggc
     3001 gatcagccgg ctcgcgggaa gcgggcgagt ggatgcgggg aagcggcctg cggatcagat
     3061 cgagccgcct gcggtttggg tttcgaagta acgggaacgc taaccgctga agcggttagc
     3121 ccgggttagg ttttcggtct ataggccgaa gttcaggatc atgtactttt gcccctcatc
     3181 cttaaccggc ttgtagtggc gttcgacgtt ccacttgttg cgatagtcga tcagcgcttt
     3241 gcgctgggcg gggtcgtcag gtagttcgac ggccatgcac aggcacatca tcgagccgaa
     3301 cgcatagcac ttcttggcgc ccatcttttc cagctcgtcg gctttgccga tggcttgtcg
     3361 ctcgcttagc tcgcccatca tgcggctggg gttctgcttg aaccacgcgt gcacttccat
     3421 cgggttttcg ttttccatct tctcggcgac gtccgggtcg tcgcctttgc cggtgctgag
     3481 ggcggcggcc gagttgcccg agcggccggc gatgattttg aacgcgaaga tgccaccgat
     3541 gaccgcggcg atgaggaaga cggggatcag gatcttcatc atctgaccct gcttgtcttc
     3601 gacaaacttc ggcttgctgc gcgcggggat gccgctgaat gccgagcccg aagggctccc
     3661 accgccacca ccacccgcgg cgggcctggc gacggtgccg ccggcgcgct tgacggtcat
     3721 cttttcgccg gtcttgaggt tgaacccgca gctgccgcag aggatggaac ccgcctgcat
     3781 cggctcgttg caagaggggc agcggtacgc gtcgtcttcc aaacccggca cggcaaccgg
     3841 ggcggcgacg gcggcggatt ccatggcggc gctcgaacga gacgccggcg cgctgtcggc
     3901 gtcggcggcg agatcgtaca ggtcatcgag gctcggttcg ttcgacgccg cgggcttcgg
     3961 cggtttgcgc acggcggcgg tctgcggggc gagctgtgac ttcacggccg gtcgagccgc
     4021 ggcaggggca gcgactgccg gcgcggcgcc ggttggcacc gcgatggact ggccgcactt
     4081 gcagcgcacc gtcttcccgg ccagttccgg cttccagacg aacttcttcc cgcacgcgct
     4141 gcagacgaag gttgaccctg tgcccgttgg catcgacttg tcctccgaga tcacccgaga
     4201 cgggacgaac aatg
//
LOCUS       DATIMH010000334         5520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3539004, whole genome shotgun sequence.
ACCESSION   DATIMH010000334 DATIMH010000000
VERSION     DATIMH010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3539004"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VLI90_05755"
     CDS             <1..1246
                     /locus_tag="VLI90_05755"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HSV13742.1"
                     /translation="PLDGIDPSFFFDDDGRTYIVHNGKPPGDKALYNGHRAIWLWAFD
                     PKTHSVSNGRIIVNGGAKLADKPVWIEGPHLFKRNGFYYLCAAEGGTSVNHSQVVFRT
                     KSLDEPFVPFEGNPILTQRDQHGQRPDPVTSVGHAQLVDTPAGDWWAVFLGVRPYERN
                     LYNTGRETFLLPVTWADEWPVILKKGEPLPRLVKRPALPASQAPTPPTTGSFTWRDEF
                     DGKELGMDWSFLRTPRERWWSLTAKPGSLLVAPRPVALNSVSEKNLELNGNPSFIARR
                     QQHMDFSAVTTLVVNAATMPSESGLAALQNDASYFFLGVKVEGGKPREIFLERHVKSS
                     QAVIARALLAADSESVDLKIEGAGKDYRFSYRPAHADAWIMLIDKVDGSILSTETAGG
                     FVGTMIGVYSRLPIENAPQAQR"
     gene            1252..3240
                     /locus_tag="VLI90_05760"
     CDS             1252..3240
                     /locus_tag="VLI90_05760"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HSV13743.1"
                     /translation="MRCSFSFFVLCFIAGHAVAATDAAKLPLLSPMFGDHMVLQRGKP
                     NTFWGCAKPGERVKVSVADRSGEAVAGDDGRWQAKLDPPEGPGPYDVTIEGSPQALRL
                     HDVLVGDVWLCGGQSNMELGLPRVTNAADELKAADHPQVRLYVVHQHVSYATAAVPAG
                     EWKVCTPASVAEGAGFSAVGYFFGAMLNQRLNVPIGLVEDCVGGTTAETWMNPDTLAK
                     HPEFSEQIAETRRLRDAGALEYGNYIMHWYDQYDAGGKNNAWAEPSLDDAGWTTVDVP
                     GHPFAGVGLPDTPAVVWFRKGITLPDPLPAGPAHLHLGQIERMDTAYVNGQFVGASAW
                     VENPRNYIVAAKLLKPGKNLIALRVLKIKPTGGFLDGPAALRLELGDKQSIALAGAWK
                     ARVSVDARPPHPMPLGFENWPSMPAVLFNGMIKPVAPLALSGAIWYQGESNAGRAAQY
                     RTLLPELIGDWRREFAQGDFPFYIVSLPAFMHRRYQPGDDGWAELREAQAMAARTVAN
                     AGLAVTIDTGEADDIHPKQKRLVGERLALLALAQHYGQPVICAGPMFTSMEHIPSDGS
                     GPAKLRLHFDHTDGGLVSKGGMPAEFSVAGADHTWHWADAKIDGDTVIVQSPEVADPQ
                     QVRYAWQSNPVATLYNGAGLPAVPFRTDAPGAPSGDAK"
     gene            3518..4213
                     /locus_tag="VLI90_05765"
     CDS             3518..4213
                     /locus_tag="VLI90_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13744.1"
                     /translation="MRATQYMSRQFPSDVGTLNALGDAQRRSDQKREAKVTFARVVAI
                     APSNSYAAFNLFDVQLELDEPDDAERTLALLRTHHAGPWADQRQIALAIRRGEKTSAQ
                     RLLGQVLAAGQAGRGALELAVKEMDGAGWTPLVDELLSEWSKSKHGAANDDLVALAEI
                     WAKRAGEAKHLRRCQRILRTLRGHQQAWLAAVVPYVNALATAKRASTLRWLRWRHRAL
                     LKSDTRAWGAVVT"
     gene            4213..4473
                     /locus_tag="VLI90_05770"
     CDS             4213..4473
                     /locus_tag="VLI90_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13745.1"
                     /translation="MTVAGKSLWRLRRVAKWMADYAVRAVARPCMMSNLTMALRALGR
                     DRQARAVSAVAVNLAPDQTTPRHVVWLALDELIANQRGPGVE"
     gene            4550..>5520
                     /locus_tag="VLI90_05775"
     CDS             4550..>5520
                     /locus_tag="VLI90_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HSV13746.1"
                     /translation="MADQYQAILDILGRAKRVLVTTHVRPDGDALGTTAAMVLGMRAK
                     GVDSHVLLLSHLPKKYEFVFKELGIAHTDVEREWPATLSLESFDTFLVVDTGTWSQLP
                     GLRERIANWRVPKLVVDHHLTQEDWADAKLVVTEAAAAGEIAAELLEQWDVELTEPIA
                     RALFLAIASDTGWFQFSNTRPFTLRLAAMLIEAGVNTDELYQLLYQNERAERVALQTR
                     AQQSLELLCDQRLAVMRVTKSDFETLRANVGDTENLINIPLQIRAVEVSLLFVEPIDG
                     GPIRVSLRSKGQVDVARFAEQFGGGGHARAAGLKVAGTMDEVHDKVVA"
BASE COUNT          949 a         1550 c         2025 g          996 t
ORIGIN      
        1 gccgctggat gggatcgacc cgtcgttttt cttcgatgac gacgggcgga catacatcgt
       61 tcacaacggt aagccgccgg gggacaaggc gctttacaac ggtcaccggg cgatttggtt
      121 gtgggcgttc gatccgaaga cgcacagcgt cagtaatggc cggatcattg tgaatggcgg
      181 tgcgaagctg gcggacaagc cggtgtggat cgaggggccg cacctgttca agcgcaacgg
      241 gttctattac ctgtgcgcgg ccgagggcgg cacgagcgtc aaccactcgc aggtggtgtt
      301 ccgcacgaaa tcgctcgacg agccgttcgt gccgttcgag gggaatccca tcctgacgca
      361 gcgcgatcag cacgggcaac ggccggaccc ggtgacgagc gtcggtcacg cgcagctggt
      421 ggacacgccc gcgggcgatt ggtgggcggt gttcctcggc gtgcggccgt atgagcggaa
      481 cctctacaac acgggccgcg agacgttctt gttgccggtg acatgggcgg atgagtggcc
      541 ggtgatcctg aagaagggtg agcctttgcc tcgccttgta aagcgtcctg cgctgccggc
      601 gtcgcaagcg ccgacgccgc cgacgacggg ttcgttcacg tggcgcgacg agtttgacgg
      661 gaaggaactg gggatggact ggtcgttcct gcgcacgccg cgtgagcgat ggtggtcgct
      721 gacggcgaag ccagggtcgt tactcgtcgc gccgcgcccg gtggcgttga attccgtcag
      781 tgaaaagaac ctggagctga atggcaatcc ctcgttcatc gcccggcgtc agcagcacat
      841 ggatttcagc gcggtgacca cgctcgtggt gaacgcggcg acgatgccga gcgagagcgg
      901 cttggctgcg cttcaaaacg acgcgagcta cttcttcctc ggcgtgaagg tggaaggcgg
      961 caagccgcgg gagatctttc ttgagcggca cgtgaagtcg tcgcaggcgg tcattgcccg
     1021 cgcactgttg gccgctgatt cggagtcggt ggacctgaag atcgaaggcg cgggtaagga
     1081 ctatcggttt tcgtatcgac cagcacatgc cgacgcttgg atcatgttga tcgacaaggt
     1141 cgatggcagc atcctgagca ccgaaacggc cgggggattt gtggggacca tgatcggcgt
     1201 ctattcgcgg ctgccaatcg aaaatgcgcc ccaagcgcag cggtgacctc catgcgatgc
     1261 tcgttttcat ttttcgtgtt gtgtttcatc gcggggcacg ccgtagccgc gacggatgcg
     1321 gcgaagctcc cactgcttag cccgatgttt ggcgaccaca tggtgcttca gcgcggcaag
     1381 cccaacacgt tctggggctg tgccaaaccg ggcgagcgtg tgaaagtgag cgtcgcggat
     1441 cgttcgggcg aagcggtcgc cggtgatgat ggtcgttggc aggcgaagct cgatccgccg
     1501 gagggaccgg ggccttacga tgtgacgatc gagggttctc cgcaggcact gcggttgcac
     1561 gatgtgctgg ttggcgacgt gtggttgtgc ggcgggcagt cgaacatgga gcttggcctg
     1621 ccgcgcgtga ccaacgcggc cgatgagctg aaggcggcgg atcatccgca ggttcggttg
     1681 tacgtcgtgc accagcacgt gagctatgcg accgcggcgg ttccggcggg ggaatggaag
     1741 gtgtgtacgc cggcgtcggt cgccgaaggg gcgggctttt ccgcggttgg atatttcttc
     1801 ggcgcgatgt tgaatcagcg gctgaacgtg ccgatcgggc tggtcgaaga ttgcgtcggc
     1861 gggacgacgg ctgagacgtg gatgaacccg gacacactgg cgaagcatcc ggaattcagc
     1921 gagcagattg ccgagacgcg ccgcctgcgc gacgcggggg cccttgagta cggcaactac
     1981 atcatgcact ggtacgacca gtacgacgcc ggcggcaaga acaacgcgtg ggcggagccg
     2041 tcgctggatg atgccgggtg gacgacggtg gatgtgccgg gtcatccgtt tgctggtgtg
     2101 gggctgccgg acacgccggc agtcgtttgg tttcgcaagg ggatcacgct gccggatccg
     2161 ctacccgcgg ggccggcgca tttgcacctg ggccagatcg agcggatgga caccgcgtac
     2221 gtcaacggcc agttcgtcgg cgccagcgcc tgggtggaga acccgcgcaa ctacatcgtc
     2281 gccgcgaagc tgctcaagcc cgggaagaac ctgatcgcgc tgcgcgtgtt gaagataaaa
     2341 cctaccggcg gatttctgga tgggccggcg gcgctgcggt tagagttggg cgacaagcaa
     2401 tcgatcgcgc tggccggtgc gtggaaggcg cgggtgagcg tggatgcacg gccgccgcac
     2461 ccgatgccgc tggggtttga gaactggcca agtatgcccg cggtgctgtt caatgggatg
     2521 attaaacccg ttgcgccgct ggcgctgagc ggggcgatct ggtatcaggg cgagtccaac
     2581 gccgggcgcg cggctcagta tcgcacgctg ctgcccgagc tgatcggcga ctggcggcgc
     2641 gaattcgcgc aaggcgattt cccgttctac atcgtcagcc tgccggcgtt catgcatcgc
     2701 aggtatcaac ccggtgacga tgggtgggcc gagctgcgcg aagcgcaggc gatggcggcg
     2761 cgcacggtgg cgaacgccgg gctagcggtc acgatcgaca cgggggaagc cgacgacatt
     2821 cacccgaagc agaagcgcct ggtgggcgag cggttggcgc tgctcgcgct ggcgcagcac
     2881 tacggccagc cggttatctg tgccgggccg atgttcacgt cgatggagca catccccagc
     2941 gacggcagcg gcccggcgaa gctgcggctg cacttcgatc acaccgacgg tggattggtt
     3001 tccaaaggtg gaatgcccgc cgaattctct gtcgccggcg ccgatcacac ctggcactgg
     3061 gccgacgcga agatcgacgg cgacacagtc atcgttcaaa gccccgaagt ggctgacccg
     3121 cagcaggtgc gctatgcctg gcagtcgaac ccggtggcaa cgctgtacaa cggcgccggt
     3181 ctgccggccg tgccgttccg caccgatgca cccggcgccc cctcgggcga cgccaaatag
     3241 cgaagttagt aagcgagcag gatcgaaaga tcggccgacg cgctcagacg gcaaacaccg
     3301 cttgggatgc accaatccaa cggcccaccc acgaactcgt actgtcgcgg atgctgtatc
     3361 aattggagtg acctcgcccc ggctgatctt ctcctcggtg gtttgaagag gttagtcaac
     3421 ttctcccgca gcactgctgt gcgatgagcc ggcgtattac tggggctggc agacgctgtg
     3481 cgaatggttg gcggatcaga aggatcgcgg ccgatacttg cgtgcgacgc agtacatgtc
     3541 gcggcagttc cccagcgatg ttggcacgct gaacgcgctg ggcgatgcgc agcggcgttc
     3601 ggatcagaag cgcgaggcga aggtgacatt cgctcgcgta gtggcgatcg cgccgtccaa
     3661 ctcgtacgcg gcgttcaact tgttcgacgt ccagctcgag ctggatgaac cggatgatgc
     3721 cgagcggacg ctggcgctgc tgcgcacgca ccacgctggc ccatgggcgg accagcggca
     3781 gatcgcgctg gcgatccgcc ggggggagaa gacgtcggcg cagcggcttc tggggcaagt
     3841 gctggcggcg gggcaggccg gccgcggtgc gcttgagttg gcggttaagg aaatggacgg
     3901 cgcgggttgg acgccgctgg tggacgagct gttgtcggaa tggtccaagt ccaagcatgg
     3961 cgcggcaaac gatgatctgg ttgccttggc ggagatttgg gcgaagcgcg cgggtgaggc
     4021 gaagcacctg cgtcggtgcc agcggattct gcggacgctg cgcggccatc agcaggcgtg
     4081 gctggcggcg gtggtgccgt atgttaacgc gctggccacc gccaagcgcg cgtcaactct
     4141 tcgctggctg cgctggcggc atcgcgcgct gttgaagtcg gatacgcgcg cctggggcgc
     4201 cgtggttacg tgatgacggt cgcggggaaa tcgctttggc ggctgcggcg ggtggcgaag
     4261 tggatggcgg attacgcggt tcgcgccgtt gcacggccgt gcatgatgag caacttgacg
     4321 atggcgcttc gcgcgctcgg tcgagatcgg caggcgcgcg cggttagcgc ggtcgcggtg
     4381 aatctggcgc cggatcagac gacgccgcgg catgtcgtgt ggctggcgct ggatgagctg
     4441 atcgcgaacc agcgcgggcc aggggtcgaa tgatttgcga gcgtatcgcg cgcgttgagt
     4501 ctgcgcatcc ccgtgcgtct ttggcggcgg gcgcttatta tcctgcccca tggcggatca
     4561 gtatcaggcg attcttgaca tcctcggccg ggcgaagcgc gtgctggtga cgacgcatgt
     4621 gcggcctgat ggcgatgccc tgggcaccac ggcggcgatg gtgctgggga tgcgggcaaa
     4681 gggggtcgat tcacacgtcc tgctgctgag ccatttgccg aagaagtacg aatttgtttt
     4741 caaagagctg ggcatcgcgc acacagatgt ggagcgcgag tggccggcga cgttgtcgct
     4801 cgaatcgttc gatacgttcc tggttgtcga taccgggacg tggtcgcagt tgccggggtt
     4861 gcgcgagcgg atcgcgaatt ggcgggtgcc aaagctggtg gtcgatcatc acctgacgca
     4921 ggaagattgg gcggacgcga agctggtggt gaccgaggcg gcggcggcgg gggagatcgc
     4981 ggcggagctg ctggagcaat gggatgtgga gttgaccgag ccgatcgcgc gggcgctgtt
     5041 tttggcgatc gcttcggata cgggatggtt tcagttctcc aacacgcggc cgttcacgct
     5101 tcgcctggcg gcgatgctca tcgaggcggg cgtgaatacc gatgagctat accagttgct
     5161 ctaccagaac gagcgggccg agcgcgtcgc gctgcagacg cgggcgcagc agtcgctgga
     5221 attgttgtgc gaccagcggc ttgcggtgat gcgcgtaacc aagagcgatt ttgagacatt
     5281 gcgcgccaac gtcggcgata cggaaaacct gatcaacatc ccgctgcaga tccgcgcggt
     5341 ggaagtgtcg ctgctgttcg ttgagccgat cgacggcggg ccgatccgcg tgagcctgcg
     5401 gagcaaaggg caggtggatg tcgcaagatt cgccgagcag ttcggcggcg gcgggcacgc
     5461 ccgggcggcg gggctgaagg tggcgggaac gatggatgaa gtacacgaca aggtcgtcgc
//
LOCUS       DATIMH010000335         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4696769, whole genome shotgun sequence.
ACCESSION   DATIMH010000335 DATIMH010000000
VERSION     DATIMH010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4696769"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1559
                     /locus_tag="VLI90_05780"
     CDS             75..1559
                     /locus_tag="VLI90_05780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSV13747.1"
                     /translation="MSDKTEHPPVPEAFAALGVRSSILRSLAEMKFETPSEIQSLLIP
                     RALAGVDILGQARTGTGKTAAFGIPILQQATKGLATQAIILVPTRELAVQVEAEIKRL
                     AQYTPIRSTPVYGGEKIAAQMKFLKHGPEIVVGTPGRVIDLLDRRIINFNNVRFVILD
                     EVDRMLDIGFRDDIRNILSRVKGVRRQLPPDVREGEGQPQQPSGEGDATPTSHQTMFV
                     SATISDEIDKLARRYMREPVEKLIAPGADEKPTVENVEQYYLSVQPWDKYRLLRMLLE
                     QENPDLAIIFCRTKHGAEKLAKKLHHDGIECREIHGNLAQKKRERVMKGFRGGKFDVL
                     VATDLASRGIDVADISHIINYDVPEDPEVYVHRVGRTARMGAKGKAFTFIGKDQGDEL
                     TKVENLINMIVPQATIEGFEARPTPSDWTEGKPGDMQLGPAKPIVNRFERPYGAAAAS
                     SSSQSTTATADATTGNAPAPAPVVLPPRTLGSKIPINRRHKRRR"
     gene            1721..2269
                     /locus_tag="VLI90_05785"
     CDS             1721..2269
                     /locus_tag="VLI90_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HSV13748.1"
                     /translation="MPKIIVVNYDPAWPAAFERIRAAVWPSIRDVASSVEHIGSTSVV
                     GLAAKPIIDATIVVPTASAMPIVIGRLATLGYQHCGNLGVYGREAFTAPPATPPHHLY
                     ACVTGSDGLHNHVTLRDYLQSNPTAAQAYSALKKQLAQQFADDIDGYTAGKSELILAI
                     LARSRFTPAQLAAIRAINSKPS"
     gene            2619..3701
                     /locus_tag="VLI90_05790"
     CDS             2619..3701
                     /locus_tag="VLI90_05790"
                     /inference="COORDINATES: protein motif:HMM:NF033816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cj0069 family protein"
                     /protein_id="HSV13749.1"
                     /translation="MKCQAAEGRDQRIGILWRGDRAAEVRPLSDECRLHRVFQVLADH
                     QVSAEPVVYCEEASAAVREQLLQLDGVLVWVNPTDGGRDRSDLNQLLRSAADRGVWVS
                     AHPDVILKMGTKEVLHRTQVVGWGDDVHRYNSVDELSLQLRCRLMAGRARVLKQNRGN
                     GGDGVWKVQLAESARDELPTAESRLRVRHALRGCIEEEMSLLAFVDRCQPYFAGGGMM
                     IDQPYQDRLPEGMIRCYLVHDRVVGFGHQATNALFPAPPGAPATAAPQPGPRLYHPPD
                     KPEFQQLKQKLEQEWVPAMQRILDIARRDLPVLWDADFLIGPKASDGGDSYILCEINV
                     SSVFPFPPNALTPLAEAVLNRLNRHP"
BASE COUNT          773 a         1306 c         1146 g          666 t
ORIGIN      
        1 cctcctgcgc acaacagcta gaatcatttc aatcgaccac aaaaacgggt cagaccacag
       61 aataggataa tgcattgagc gataagaccg agcacccgcc tgtgcccgaa gcgtttgcgg
      121 ctttaggcgt tcgatcgtcc atccttcgtt cgcttgccga aatgaagttc gaaacgcctt
      181 cggaaattca gagcctgctg atcccgcgcg cgctcgccgg tgtggacatc ctcggtcagg
      241 cccgcaccgg caccgggaaa accgcggcct tcggtattcc cattctgcaa caagccacta
      301 agggactggc cacgcaggcg atcatcctcg tcccgacgcg cgagttggcc gtgcaggtcg
      361 aggcggagat caagcggctg gcgcagtaca cacccatccg ctccaccccg gtgtacggcg
      421 gtgagaaaat cgcagcgcag atgaagttcc tcaagcacgg ccccgaaatc gtcgtcggca
      481 cccccggccg cgtgatcgac ctgctcgacc gccggatcat caacttcaac aacgtgcgtt
      541 tcgtcatatt ggacgaagtc gatcgcatgc tggacatcgg cttccgtgac gacatccgca
      601 acatcctgtc gcgcgtcaaa ggcgtgcgcc gccagctgcc gccggacgtg cgcgaaggag
      661 aagggcaacc gcaacaaccc tctggcgaag gcgacgcgac gccgacctcg catcagacca
      721 tgttcgtctc ggccacgatc agcgacgaga tcgacaagct cgcccggcgt tacatgcgcg
      781 agccggtcga aaagctaatt gcccccggcg ccgatgaaaa gccgacggtt gagaacgtcg
      841 agcagtatta cctgtcggtc cagccgtggg acaagtaccg cctgctgcgg atgctgctgg
      901 agcaggagaa ccccgacctg gccatcatct tctgccgcac caagcatggc gcggaaaagc
      961 tggccaagaa gctgcatcac gacggcatcg aatgtcgcga aatccacggc aacctcgcgc
     1021 agaaaaagcg cgagcgcgtg atgaaaggtt tccgcggcgg gaagttcgac gtgctggtcg
     1081 ccaccgatct ggcgtcgcgc ggcatcgacg tcgccgacat cagccacatt atcaactacg
     1141 acgtgccgga agaccctgag gtgtacgtgc atcgcgtcgg ccgaacggcc cgcatgggcg
     1201 ccaagggcaa ggcgttcacg ttcatcggca aagatcaggg cgacgagctg accaaggtcg
     1261 agaacctgat caacatgatc gtcccccagg ccaccatcga aggcttcgaa gcccgcccaa
     1321 ccccgtccga ctggaccgaa ggcaagcccg gcgacatgca gctcggcccg gccaagccga
     1381 tcgtcaaccg cttcgaacgt ccctacggcg cggccgcggc gtcatcctca tcgcaatcaa
     1441 ccaccgcgac cgccgacgcc accactggaa acgcccccgc gcccgccccg gtcgtcctgc
     1501 caccgcgcac gctgggcagc aagatcccca tcaaccgccg ccacaagcgc cggcggtgaa
     1561 gcgcgcgaag gagcgcctag cccgcgcgaa agtctctccg ccgcgtagcg gtagccccga
     1621 aggggcacgt gtttttccct cggacgaaaa cgcgtacccc ttcggggcta ccgctacgcg
     1681 gcagaagtct ggatgaccgc ccagcgccaa gcttcccccc atgcccaaga tcatcgtcgt
     1741 caattacgac cccgcctggc ccgcggcctt cgagcgcatc cgcgctgctg tctggccgtc
     1801 cattcgcgac gttgcatcct ccgtcgagca catcggcagc acatcggtcg tcggcctggc
     1861 ggccaagcca atcatcgacg cgaccatcgt cgtgcccacg gcgtcggcga tgccgatcgt
     1921 catcggtcga ctcgcaacgc tcggctacca acactgcggg aacctgggcg tgtatggccg
     1981 cgaggcgttc accgcgccgc ccgccacgcc gccgcaccac ttgtacgcct gcgtcaccgg
     2041 cagcgacgga ctccataacc acgtcaccct tcgcgattat ctgcaaagca atcccaccgc
     2101 ggcacaggca tacagcgcgc tgaagaagca actggcccaa caattcgccg acgacatcga
     2161 cggctacacc gccggcaaaa gcgaactcat cctggcgatc ctggctcgct cccgcttcac
     2221 gccggcgcag ctcgccgcga tccgcgccat caacagcaag ccgagttgac tacgagcgtt
     2281 cgacggtgcg gcctgtagct gcgacgcctg cgtcgccggt cttgtctgtc cgctcgcgca
     2341 tcgtcacccc tttttctgcc gatcttgcgg cactagtatc tttctccgca ggagaatctc
     2401 tatgcacgtc cgcgtttcgc atgtaagtgg ccgaagcgct ccgcccgctg aaccgctggt
     2461 actgtgccag gcccagatgt aaaaacgtaa ctctcaagat ttgcgctaga ccttgagcgc
     2521 ccgctcttcc gttgctcctt ttcttaggtt tcttcggcca gctgccgtag ccgcaacaac
     2581 atccgaagca agcgctcgac gatggaggct cacaactcat gaaatgccaa gcagccgaag
     2641 ggcgtgatca acgcatcggc atcctatggc ggggcgatcg ggcggccgaa gtgcggccgc
     2701 tgtccgacga atgccgcctg caccgggtgt ttcaagtgct cgccgatcat caggtttcgg
     2761 ccgagccggt ggtttattgc gaagaagcgt cggccgcggt ccgcgagcag ttgctgcaac
     2821 tggacggcgt gctggtgtgg gtgaatccga ccgacggcgg tcgcgatcgc agtgacttga
     2881 accagctact tcgcagcgca gcagaccgcg gcgtttgggt cagcgcccat cccgatgtga
     2941 tcctgaagat ggggacgaaa gaagttctcc atcgaacgca ggtggttggc tggggagatg
     3001 atgtccatcg atacaacagc gttgatgaac tgtccctcca attgcgctgc cggttgatgg
     3061 cggggcgggc tcgcgtgttg aaacagaacc gcgggaatgg cggcgacggc gtttggaagg
     3121 tgcagctcgc ggaatcggct cgtgatgaat tgccgaccgc cgaaagccgc ttgcgcgtcc
     3181 gacatgcgct gcgcggatgc atcgaggaag agatgtcgct gctcgcgttc gtcgatcgtt
     3241 gccagcccta tttcgccggc ggcgggatga tgatcgacca gccatatcaa gaccggctgc
     3301 ccgaaggcat gattcgatgc tacctcgtgc acgaccgcgt cgtcggattc gggcaccagg
     3361 cgacgaacgc gctgttccca gcgccacccg gcgcgccggc gaccgccgcg ccgcagccgg
     3421 ggccgcggct ctatcacccg ccggacaaac cggaattcca gcaattgaaa cagaagctcg
     3481 aacaggaatg ggtgccggcg atgcagcgga tcctcgacat cgcccgccgc gatcttcccg
     3541 tgctctggga tgccgatttt ctgatcggac ccaaggccag cgacggcgga gattcttata
     3601 tcctttgcga aatcaacgtc agctcggttt tcccattccc gccaaacgcg ctgacaccgt
     3661 tggccgaagc cgtgttgaac aggctgaatc gacacccata gcgaggaaca ctgatgcaac
     3721 atcgaaccat tgccgctttg gccgtgctga ctgccatcgc cgcggtgctc gcctcggcgc
     3781 gactctttgg cgccgagccc gtggcgtcgt cattgtcgtt ggttcggacg atttccctcg
     3841 acggtgtcga gggtcgaatc gaccacatgt cgatcacgcc ggatgggcgg c
//
LOCUS       DATIMH010000336         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4405356, whole genome shotgun sequence.
ACCESSION   DATIMH010000336 DATIMH010000000
VERSION     DATIMH010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4405356"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VLI90_05795"
     CDS             complement(<1..883)
                     /locus_tag="VLI90_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV13750.1"
                     /translation="MESKPVTPERVMQMAFAFSAPLMIEAAIEHRVFDLLDQGPKTVD
                     EVAKAGGASVRGLRGIMDALVGLEFLQRDGNRYKLAADTQQFLVSTKASFLGNVFKHM
                     NRQLMPKWMQLNEIVRTGKPAYSVNEQGDGSAFFAQFVESIFPMSYPAASALGDALKL
                     STTEKPISVLDLAAGSGVWGIALAQKSPKVRVTAVDWPNVLEVTRRVVERFKLGDRFT
                     YSPGDLATADFGSGHQVATLGHILHSEGERRSRELLKKTFKALAPGGTISIGEFNPNE
                     ERTGPPQALIFAVNMLVN"
     gene            1101..3833
                     /locus_tag="VLI90_05800"
     CDS             1101..3833
                     /locus_tag="VLI90_05800"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSV13751.1"
                     /translation="MRALLIGMLITALVIVLDGAGKLQRLEWALYDARVRLCQFFRKL
                     PTDRLVHVDIDDSALETIGRWPWPRAILADVVDELGRAGTKAVAFDVLLSEPEKPEWH
                     EDHTVTDHDRIFADAVRKMGHVLVPASLTFEDQTTAIQLVPAAELHLEQNLELSFDEL
                     NQLLLASHLPRVTQDEYAHAQKTAMATRVQRALDAEALTSQQLRQRLLPTISRWFPNH
                     PAIAVLDKIELQYRAQRAFERLTLPMTAATAHIPLLHGIGVIAALPEFSAAARFSGIV
                     DNPAMPDGVVRAIPLFAEFHNRLAPQIGLVLACAALDVDPQQLLIQPGQVQVPLADGF
                     NINIPVHELPTRFGRGGLFMDVPWFGGKDWISIYGAPGRQHVNISVLYELAGWRRRLS
                     ANNQIIDESLLYLMNQSLDLAPVQEKLAAAVKGMAADDFAARRDYVARTLGNEELKQG
                     VPALPADDKIRIAWDNLVSAANQNERLDALARARSAQFRSMFENHVVLIGNTASANTD
                     MVNTPLAPQMPGVIAHGVVVNAILTRQMWREAPFFVTALYTLGCGLIATLAVAFLGPF
                     RSAMSVIALLLGFLLLNGIVLFDRGNYLLGTAGPVVVIVLVWGVENLISVITETAERA
                     RLDNRFRSRTDPQLVDFLHEHPEQTRLSGQRKELTVVFTDLEGFTAASEKLREETVQV
                     LNEYFRRMVPLIRRRKGFVNKLLGDGIMFFFGAPFDNPNHATDAIETVLDMVAETERF
                     STDLVARGLPPVVMRAGINSGIMTVGDVGGEAYSDYTLIDDNVNTAARLEKANKRFDA
                     RVMIGGNTYRHLDSTRFALRPVGALMLAGKSESVQAYEVLARSADATITQRQICRLSE
                     PVVATFAAGDVAGCLEAIERMRVELGQTKFLAAYEQLCRSAEGENASVQKYVFA"
BASE COUNT          709 a         1241 c         1267 g          724 t
ORIGIN      
        1 tgttcaccag catgttcacc gcgaagatca gcgcctgcgg cggaccggtg cgctcttcgt
       61 tcggattgaa ctcgccgatc gaaatcgtcc cgccgggggc cagggctttg aaggttttct
      121 tcagcagctc gcggctgcgg cgttcgcctt cgctgtgcag gatgtgaccg agcgtcgcga
      181 cctggtgccc gctgccgaag tcggcggtgg cgagatcgcc cggggaatac gtgaagcgat
      241 cgccgagctt gaaacgctcg acgacgcgcc gcgtgacctc cagcacgttc ggccaatcga
      301 cggccgtcac gcgcaccttt ggcgacttct gggccagcgc aataccccac acgccggaac
      361 cggcggcgag atccagcacg ctgatcggct tctcggtcgt cgaaagtttc agcgcgtcac
      421 ccaacgccga cgcggcgggg tagctcatcg gaaaaatcga ctcgacgaac tgcgcgaaga
      481 acgcgctgcc gtcgccttgt tcattgaccg agtacgcggg cttgcccgtg cggacgatct
      541 cgttcagctg catccacttc ggcatcagct ggcggttcat gtgcttgaag acgttgccga
      601 gaaaactcgc cttggtgctg acgaggaact gctgcgtgtc ggcggccagc ttgtatcgat
      661 tgccgtcgcg ctgcaggaac tcgagcccga cgagtgcatc catgatgccg cgcaggccgc
      721 gcaccgatgc gccgccggcc ttggcgactt cgtcaacggt cttcggcccc tggtcgagca
      781 ggtcgaagac gcgatgttcg atggccgcct cgatcatcag cggcgcggaa aacgcgaacg
      841 ccatctgcat gacgcgctcg ggcgtgacgg gttttgattc catggacgtg cctcctgttt
      901 ggttggcccg cgcctatcga taccgtcgcg gggggtgaag ttcaattcga aagtacctca
      961 gacaccggcg tcgggcgcgt gcgcgcatgt ctcaatcgtg aattgcacgc tccgaccgta
     1021 taatcccgct tcacttgaca gacgcgggcg atccaactca acgacgcaag cgacgaacgc
     1081 gcaacgagcg ccggcaggcg atgcgcgcac tgctgatcgg catgctgatc accgcgctgg
     1141 tcatcgtgct cgatggcgcc gggaagctgc agcggctgga atgggccctg tacgacgccc
     1201 gcgtgcggct gtgccagttc ttccgcaagc tgcccaccga tcgcctcgtt cacgtggaca
     1261 tcgacgacag cgcgctggag acgatcggcc gctggccgtg gcctcgcgcc atcttggcgg
     1321 atgtcgtcga tgaactcggc cgcgccggca ccaaggcggt cgcgttcgac gtgctgctgt
     1381 cggaaccgga aaaacccgag tggcatgaag accatacggt gaccgatcac gatcggattt
     1441 tcgccgacgc ggtgcgaaaa atgggccatg tgctggtgcc ggcgtcgttg acgttcgagg
     1501 accagacgac cgcgattcag cttgttccgg cggccgaact gcatctggaa cagaacctgg
     1561 agctgtcctt tgatgagctg aaccagctgt tgctggcgtc gcacctgccg cgggtgacgc
     1621 aggacgaata cgcgcacgcg cagaagacgg cgatggcgac gcgagtgcag cgcgccctcg
     1681 acgcagaagc gctcaccagc caacagctgc gccagcggct gttgccgacc atcagccgat
     1741 ggtttccgaa tcacccggcg attgccgtgc tggacaagat cgagctgcag tatcgcgctc
     1801 agcgcgcgtt cgaacggctc acgctgccga tgacggcggc gaccgcgcac atcccgttgt
     1861 tgcacggcat tggcgtcatc gcggcgctgc cggaattctc ggcggcggcg cggttcagcg
     1921 gcatcgtcga taacccggcc atgcccgatg gggtggttcg agccatcccc ttgttcgcgg
     1981 aatttcacaa tcgcctggcg ccgcaaatcg gcctggtgct ggcgtgcgca gcgctggatg
     2041 tggacccgca gcagctgctc atccaaccgg gccaggtgca ggtgccgctg gccgatggat
     2101 tcaacatcaa cattcccgtc cacgagctgc ccacccgatt cggccgcggc gggttattca
     2161 tggacgtgcc gtggttcggt gggaaagatt ggatatcgat ctacggggca cccggccgtc
     2221 agcacgtgaa catcagcgtt ctttacgagc tggccggatg gcgccgccgg ttgagcgcca
     2281 acaatcagat catcgatgag tcgctcttgt acctgatgaa tcagtccctg gacctggcgc
     2341 cggttcagga gaaactcgcc gcggcggtga agggaatggc ggcggacgat tttgccgccc
     2401 ggcgcgatta cgtcgcgcga acgcttggca acgaagagct caagcaaggc gttccggcgc
     2461 tgccggcgga cgacaagatc cgcatcgcct gggacaacct cgtctcagcg gccaaccaga
     2521 acgaacggct tgatgcgctg gcgcgggcgc ggtcagcgca gttccgctca atgttcgaga
     2581 accacgtcgt cttgatcggc aacaccgcca gcgccaatac cgacatggtc aacaccccgc
     2641 tcgccccgca gatgccgggc gtgatcgccc atggcgtcgt ggtcaacgcg atcctgaccc
     2701 ggcagatgtg gcgcgaagca ccgttcttcg tcaccgcgct ttacacgctg ggctgcgggt
     2761 tgatcgcgac gctggcggtt gcgtttctcg ggccgtttcg ttcggcgatg tcggtcatcg
     2821 cgctgctgct cggcttcctg ctgctcaatg gcatcgtgct gttcgaccgc gggaattacc
     2881 tgctcggcac cgccgggccg gtggtggtga tcgtgctggt gtggggtgtt gagaacctta
     2941 tctcggtgat tacggagacc gccgagcgcg cccggctcga caatcgcttc cgcagccgaa
     3001 ctgacccgca gctggttgac ttcctgcacg agcatccgga acagacgcgc ctcagcggtc
     3061 agcgcaagga actcaccgtt gtcttcaccg atctggaagg ttttaccgcg gccagcgaga
     3121 agctgcgcga agagaccgtg caggtgctga acgaatattt ccgccggatg gtcccgctga
     3181 ttcgccggcg aaagggtttc gtcaacaagc tgctcggcga cggcatcatg ttcttcttcg
     3241 gcgcgccgtt cgacaaccca aaccacgcga cggacgccat cgaaacggtg ctggacatgg
     3301 tggccgagac ggagcggttt tcgactgacc ttgtcgcgcg cggcctgccg cccgttgtga
     3361 tgcgcgcggg catcaacagc gggattatga ccgtcggcga cgtgggcggc gaggcgtaca
     3421 gcgactacac gctgatcgac gacaacgtga acaccgccgc ccgattggag aaggcgaaca
     3481 aacgcttcga cgcccgcgtg atgatcggcg ggaacaccta tcggcatctg gactcgacgc
     3541 gtttcgcgct ccggccggtc ggcgcgctga tgctcgcggg aaaatccgag tcggtgcaag
     3601 cgtacgaagt gctcgcgcgc agcgctgatg caacgatcac acagcggcag atctgccgat
     3661 tgagtgagcc cgtcgtcgcc acgtttgccg cgggcgatgt ggccgggtgt ctcgaggcga
     3721 tcgagcggat gcgcgtcgag cttggccaga caaagttctt ggccgcctac gagcagcttt
     3781 gccgctcggc cgagggtgag aacgcgtcgg tgcagaagta cgtttttgcc taatttcgca
     3841 aaacgccgag aaccctgacc ctccattccg gtaggtggct gcacagtaaa accgcatctg
     3901 cacgatttgc acctaatatc cacacgcatc catgaacaat c
//
LOCUS       DATIMH010000337         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1808587, whole genome shotgun sequence.
ACCESSION   DATIMH010000337 DATIMH010000000
VERSION     DATIMH010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808587"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..518)
                     /locus_tag="VLI90_05805"
     CDS             complement(69..518)
                     /locus_tag="VLI90_05805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV13752.1"
                     /translation="MAQADIIIADDKLLPQGVELYNSIFRPKREVDFFKRRFMGRYNA
                     LTLIARMDDKPVGFWIGFELKPGMFYHWLGAVVPDIRRHGIGRQLQEAQQAWAKDHGY
                     EYIRCECMNHKREFIHFAVEMGYDIVGIRWDSTHADNLVVFEKNIIE"
     gene            complement(720..2129)
                     /locus_tag="VLI90_05810"
     CDS             complement(720..2129)
                     /locus_tag="VLI90_05810"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV13753.1"
                     /translation="MQPPQQPKAYEPPDPERPASAALTEPELEREMPGGHDTGGHDPY
                     AALRVPGYRLYALGTILLFMGQQAQSVAIGWELAVRDPKHAAMALSFVGLVGAAPVIL
                     LALPAGQLADRIDRKRLVIAMAILAAMCSVGLSIFSYQHWPTSYMYGLLLLGATAWAL
                     GGPARSALAPQIVPINVFSNAMMWAATFFQVSAISGPALAGWIIGWEYVHLRGRTLPT
                     LPLAYLIDAFSGVAFAILLLFVRVRPIETRKEPASLKTLLAGIHFVGRNQIILATITL
                     DLFAVLLGGATYLLPIYATSILHVGAQGFGWLRAAPALGAATMAMAIAHLPPMKRAGR
                     DLLLAVIGFGVATIVFGLSKSFWLSLAMLYLTGAFDNISVVVRHTLVQVLTPDEMRGR
                     VSAVNNVFIGASNELGGWESGLTAQIFGPVISVVGGGIGTILVVIATALTWPRLRQFG
                     SLHDAKPIEEAEVPVATSS"
     gene            2429..>3546
                     /locus_tag="VLI90_05815"
     CDS             2429..>3546
                     /locus_tag="VLI90_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSV13754.1"
                     /translation="MDLLKYSPFPDLAAALRARLDSILQRWGELVVQKLPGADELTLE
                     QLRDHLPAVLEKIAVALESKGGESMSDLREVSESHGETRFHQSFNVNELLVEYGLLRP
                     VVIDEVMAHLGRDATRDEIAVLNMSLDAAVRRSVTQFLAYQEKQLKTITGAQSKYLSF
                     LSHDLRGGLNGVLLMAEVLKRELANEPRFAESVEDLDAMRRSILDTVATMDRFLHAER
                     FRQGKVQPKNSTVDLRAVTADLSHQLSHQAKEKGIDLDFKFKNGSVAHTDKDLLALIL
                     QNLIGNAIKYTPRGSVRVVVEPPADGRGVRIAVQDQGPGISPEHLANLFEPFQRGETH
                     GQPGVGLGLSIAHQAAELIGAKLRATSTVGKGSTFTLDL"
BASE COUNT          700 a         1173 c         1082 g          591 t
ORIGIN      
        1 gatcatgttc gcctacacga ccaagccgtt catcgccggc gtcacctccg gcgcgttcaa
       61 agcctgaatt attcgatgat gttcttctca aacaccacga gattgtccgc gtgcgtcgaa
      121 tcccaccgga tcccgacgat gtcgtatccc atctcgaccg caaaatgaat gaactcgcgc
      181 ttgtggttca tgcactcgca gcggatgtat tcgtacccgt gatccttggc ccacgcctgc
      241 tgcgcttcct gcagctgccg gccgatgcca tggcggcgga tgtccggcac caccgcgccc
      301 agccagtgat aaaacatccc gggcttcagc tcaaagccga tccagaatcc caccggcttg
      361 tcgtccatcc gcgcgatcag cgtcagcgcg ttgtatcggc ccatgaagcg gcgtttgaag
      421 aagtccacct cgcgcttggg ccggaagatg ctgttgtaca gctccacgcc ctgcggcagg
      481 agcttgtcgt ctgcgatgat gatgtcggcc tgagccacgg aaacgaacct ccaaagacgc
      541 aggcggagca agcgctgcga gtgcgcgatt gtaaccacgg cgcggcaaga ttccatcggc
      601 aggttacgtg cggaggaatc gccttaacag ccgcaggcaa aagccagcgg gggcgttaac
      661 ttttcgagcg atttgcgaat tccaaaatcc cgctggctgt cgcctgcggc tacgaaagat
      721 taggaggaag tcgcaactgg aacctccgct tcctcaatcg gcttcgcgtc atgcagcgac
      781 ccgaactgtc gcagccgcgg ccacgtcagg gcggtggcga tcacaacgag gatcgtccca
      841 atgccgccgc caaccacgga gatgacgggg ccgaagatct gcgcggtcaa tccagattcc
      901 cacccgccca gctcgttgct cgcgccgatg aagacgttgt tgactgctga cacgcggccg
      961 cgcatttcgt ccggcgtcag cacctgcacc agcgtgtggc gaacgacgac gctgatgttg
     1021 tcgaacgcgc cggtcaggta gagcatcgcc agcgacagcc agaagctttt cgacaaaccg
     1081 aagacgatcg tcgcaacgcc gaacccgatg acggccagca gcaggtcgcg gccggcgcgc
     1141 ttcatcggcg gcaggtgcgc gatggccatc gccatcgtgg cggcgcccaa tgccggcgcg
     1201 gcccgcagcc agccgaagcc ttgggcgccg acgtgcagga tgctggtggc gtaaatcggc
     1261 aacaggtacg tcgcaccgcc gaggagtacc gcgaacagat cgagcgtgat cgtcgcgagg
     1321 atgatttgat tgcggccgac gaagtgaatg cccgcgagca gtgtcttgag gctcgccggt
     1381 tccttgcgcg tttcaatcgg gcggacgcgc acgaacagca gcaggatggc gaacgcgacg
     1441 ccgctgaacg cgtcgatcag gtacgccagc ggcaaggtcg gcagcgttcg gccgcgcagg
     1501 tgcacgtatt cccagccgat gatccacccg gccagcgccg ggccagagat ggccgacacc
     1561 tggaagaagg tggccgccca catcatcgcg ttgctgaaga cgttgatcgg gacgatctgc
     1621 ggcgccagcg ccgatcgcgc cggcccgccc agcgcccacg cggtggcgcc cagtagcagc
     1681 aagccataca tataagatgt cggccaatgc tggtacgaga agatcgaaag ccccacggag
     1741 cacatggccg ccaagatcgc catagcaatc acgagccgct tgcgatcgat gcgatccgcc
     1801 agctgacccg ccggcagcgc cagcaagatg accggcgcgg cgccgaccag gccgacgaag
     1861 ctcagcgcca tcgccgcgtg cttcgggtcg cgcacggcca gctcccaacc gatggccacg
     1921 ctctgcgctt gctgacccat gaacagcagg atcgtgccca gcgcgtacag ccgatatccc
     1981 ggcacacgca gcgcggcgta cggatcgtgc ccgccggtgt catgcccgcc cggcatttcg
     2041 cgttccagtt ccggctccgt cagcgccgcc gacgccggtc gctcggggtc gggcggttca
     2101 tatgctttag gttgctgcgg cggctgcatg tgctggcttc actcccacgt cgcctccacc
     2161 agcggaacag ccgcctgcga gggggacgga agtttagtcg cacatccccc gcgtggcgag
     2221 ccagcgagcg cgatccgcgc gcctgcgcct ggcgcgccag ccgatgacgc tggtgtcctg
     2281 aggtattgca accctcagtt attccgtcca aacaacgaac acagcttgac gtactaccac
     2341 ttcatgggaa aattcgaccg gctatcaggg ctatcagttt cgtgcggcga tcgcgcgcgg
     2401 gagacaacgg atgttagata atccgacaat ggatttgctg aagtattccc ctttccccga
     2461 tctggccgcc gcgctgcgcg cgcggctcga ttcaatcctg cagcgatggg gcgagctggt
     2521 cgtccaaaag ctgccgggcg cggacgaact cacgctcgaa cagctgcgtg atcacctgcc
     2581 ggccgtgctg gagaagatcg ccgtcgcgct ggaatccaag ggcggcgaat cgatgtccga
     2641 ccttcgtgaa gtctccgaat cccatggcga aacgcgcttt caccagagct tcaacgtcaa
     2701 cgaactgctc gtcgagtacg gcctgcttcg gccggtcgtg atcgacgaag tcatggcaca
     2761 cctgggccgc gacgcgacgc gcgatgagat cgcggtgctg aacatgagcc tcgatgccgc
     2821 cgttcggcgg agcgtcacac aattcctcgc ttaccaggaa aagcagctca agacgatcac
     2881 cggcgcgcag agcaagtacc tctcgttcct gtcgcacgac ctccgcggcg ggttgaacgg
     2941 cgtgctcttg atggcggaag tgctgaagcg cgaactggcc aacgagccgc gcttcgccga
     3001 atcggtcgaa gacctcgacg cgatgcggcg gtcgatcctc gacaccgtcg cgacgatgga
     3061 ccgcttcctg cacgccgagc gcttccgcca gggcaaggtc cagccgaaga acagcaccgt
     3121 tgaccttcgc gccgtcaccg ccgacctttc gcatcagcta tcacaccagg cgaaggagaa
     3181 ggggatcgat ctcgatttca aattcaaaaa tggctcggtc gcgcacaccg acaaggacct
     3241 gctggcgctg atcctgcaga acctgattgg caacgcgatc aagtacacgc cgcgcggcag
     3301 cgtgcgcgtg gtcgtcgaac cgcccgccga cggccgtggc gtccgcatcg cggtgcaaga
     3361 ccagggcccg ggcatctcgc cggagcatct ggcgaacctc ttcgaaccct tccaacgcgg
     3421 cgaaacccac ggccaacctg gcgttggcct cgggctctcc atcgcgcatc aagccgctga
     3481 attaatcggc gcgaaactgc gtgcgacatc caccgtcggc aaaggctcga cattcacgct
     3541 ggatct
//
LOCUS       DATIMH010000338         1893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3251381, whole genome shotgun sequence.
ACCESSION   DATIMH010000338 DATIMH010000000
VERSION     DATIMH010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3251381"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VLI90_05820"
     CDS             complement(<1..183)
                     /locus_tag="VLI90_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13755.1"
                     /translation="MSMNLSDKDILDLNELCNAVVEGTITDDQRSELSHWLITSEDAR
                     QFYVRAMSLSASMCCYA"
     gene            complement(195..755)
                     /locus_tag="VLI90_05825"
     CDS             complement(195..755)
                     /locus_tag="VLI90_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV13756.1"
                     /translation="MERDRNEQETESRKRLMALMTRHQRQIFSYIYVLVPNRNDAEDI
                     LQETSLLICEKFHEFKEGTDFVAWASQIAYWRVRYSRQKFARSKVVFDQEIVDVLAQT
                     ASSMADEMDDRHEALGRCLQQLHPRDRELLIARYEPGGGVEEAARRSGRTLPTAYKAL
                     ARLRKLLLDCVTTRIANESGGPATAT"
     gene            complement(853..>1893)
                     /locus_tag="VLI90_05830"
     CDS             complement(853..>1893)
                     /locus_tag="VLI90_05830"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSV13757.1"
                     /translation="VRGIRIFAAAALLATTPMIFAADAPSKSAEATHVKKVEKPPNFI
                     PAKNDPNVGDYQGDGSGQVVAQVVVAPGGGYTANLLKAFDTENNLIATLHGSADNLTG
                     DGWTGSIKSSHFTGNKGGQKFDIQHIERTSPTLGAKPPAGAVVLFDGKNMDAWAKKSG
                     KDWLTEDGPSKWKLVPEENAMEVVPESDCIITHQKFGDCHLHAEFRTLGTPSNSGVFL
                     EDRYEANINETYGRTDGTPNGGFDNCTDKVEPKIRPSFPPLAWQTFDIDFHAPRFDGA
                     NKTTDATATVLLNGVKIYDNQKLDPPHGAAGRLREAPTGPLMLQEHGMPVQFRNIWIV
                     PAAGATASATGG"
BASE COUNT          323 a          548 c          608 g          414 t
ORIGIN      
        1 ggcataacag cacatgctgg cggacaagct catcgcgcgc acgtaaaact gtctggcgtc
       61 ttctgaagtg atgagccaat gggacaactc gctgcgctgg tcgtcggtga tcgtaccttc
      121 gacgacggcg ttgcacagtt cattgagatc gaggatgtcc ttgtcgctga ggttcatgga
      181 cattgacgaa atgtttacgt ggccgtcgcg gggccaccac tttcattggc gatacgcgtc
      241 gtcacgcaat ccagcagcag cttgcgcaac cgcgccaacg ctttataggc cgttggcagc
      301 gtgcggccgg atcggcgagc ggcttcttcg acgccgccgc cgggttcgta tcgggcgatc
      361 agcagctcgc ggtcgcgcgg gtgcagttgt tgaagacatc ggccaagcgc ctcgtggcgg
      421 tcgtccattt cgtcggccat ggaactggcg gtctgggcca gcacgtccac gatctcctgg
      481 tcgaacacga ctttggagcg ggcaaatttc tgcctggaat accgcacccg ccaataggcg
      541 atttgcgagg cccaggcgac gaagtcggtg ccttctttaa attcgtgaaa tttttcgcaa
      601 atcagcaggc tggtttcctg caggatgtcc tcggcatcgt tacggttggg caccagcacg
      661 tagatgtacg aaaagatctg ccgctggtga cgtgtcatca gcgccatcaa gcgcttacga
      721 ctttcggtct cttgttcgtt gcgatcgcgc tccatcgacg tgcgctccca caaacggggg
      781 aaaaacaatc gctctgcagt cctaatagca attgcccatc aagattagga caggcaaagc
      841 cgcgggcgcg gctcaaccac cggtggcgct ggcggtggcg ccggccgcgg ggacgatcca
      901 gatgttccgg aactgaaccg gcatgccgtg ttcctggagc atcagtggtc ccgtcggcgc
      961 ttcacgcaac cgcccggcgg caccgtgcgg gggatccagc ttctggttgt cgtaaatctt
     1021 cacgccgttc agcagcaccg tcgccgtggc gtcggtggtc ttgttggcgc catcgaaccg
     1081 aggggcgtgg aagtcgatgt cgaacgtctg ccaggccagc ggcggaaagc tggggcggat
     1141 cttgggctcg accttatcgg tgcagttatc gaacccaccg ttaggcgtgc cgtcggtccg
     1201 gccgtaggtc tcgttgatgt tcgcttcgta gcgatcttcg aggaacacac cgctgttgct
     1261 cggcgtgccg agcgtgcgaa attccgcgtg cagatggcag tcgccgaatt tttgatgcgt
     1321 gatgatgcag tcgctctcgg gcacgacctc catcgcgttt tcttccggca ccagtttcca
     1381 cttcgagggg ccgtcttcgg tcagccagtc tttgccggac ttcttggccc aggcgtccat
     1441 gttcttgccg tcgaagagta cgaccgcgcc cgcgggcggc ttggcgccta gcgtgggaga
     1501 ggttcgctcg atgtgctgga tgtcgaactt ctggccgccc ttgttgccgg tgaagtgcga
     1561 ggacttaatc gagccggtcc aaccgtcgcc ggtgaggttg tcggccgagc cgtgcaacgt
     1621 ggcgatgagg ttgttctctg tgtcgaaggc cttgagcaga ttagcggtgt aaccgccgcc
     1681 gggggcgacg acgacttggg cgacgacctg gccgctaccg tcgccctggt aatcacccac
     1741 gttgggatcg ttcttggccg ggatgaaatt cggtggtttc tcgaccttct tgacatgcgt
     1801 ggcttcggcg gatttcgacg gcgcgtcggc cgcgaagatc attggcgtgg tggccagcag
     1861 agcagccgcg gcgaagattc gaatgcctct cac
//
LOCUS       DATIMH010000339         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1809092, whole genome shotgun sequence.
ACCESSION   DATIMH010000339 DATIMH010000000
VERSION     DATIMH010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809092"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..429)
                     /locus_tag="VLI90_05835"
     CDS             complement(160..429)
                     /locus_tag="VLI90_05835"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HSV13758.1"
                     /translation="MNISLDPKLARFVEERVKAGLYDSADDLINAAVARLKTDEEKAA
                     EELADLRVDVDAGLAEADRGDFAEFTAEDVIAERHAARASRHLGT"
     gene            complement(500..3334)
                     /locus_tag="VLI90_05840"
     CDS             complement(500..3334)
                     /locus_tag="VLI90_05840"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSV13759.1"
                     /translation="MNRSRSLVLTLLLLLCLPLRSATAQQAEPIQYQEQTLPNGLRVI
                     YAPLHQAPVVHVRVLYHVGSRDERPDRQGFAHMFEHMMFRGSAHVAPQQHMKLVNEVG
                     GYSNAFTSFDQTTYVNTVPAENLEMVLYLEADRMSSFKVNDEIYRTERKVVAEEWGIK
                     QNRPYGNLYEDYLKTAFTTHSYRWTPIGNMQQLAAAPSSELQEFFNTFYVPNNAVLVV
                     AGDIDVDAAKAMVQKYFGWIPKGADVPRRAEPEPPQTQPRNATIPDRVPLTQIVAGWH
                     TPDYQSDDNYALSVLSAILGEGASSRLERLLVTSEHPLCDSAETIFFNLEDAGIFGVS
                     GRVMPGKDAGEVERVLKSAVADVVANGVSDEEVAKARTLRRVEVIHNRLTAENLATQL
                     GEEAMFGGDPSRVNRELAKIDAVTAADIKAIAAKYLQPDGCTTVRMVPDPLGIEARKA
                     ATMAVAELSKKDAPVAPSTEPIQARVVQFPGGYPAQPPMADPRSAAVFQKGTEQTING
                     VRVVVMPDHRLPLVNWNLAIRRGSHCDPSGKEGLADLTAHMLRRGAGNLTEHQLSEEL
                     ESHGISLEVRDDGDITRLVGSTTTEELDRGFARSRDVLLSPTFPADEFEKVKEQSLSG
                     LTAALENPSTVADMDLLRALFGGTPLGHPATPASLSRITLDDVKQFYAEIYHPNDAVL
                     IISGDVTVERGQALAKQLTDGWQPKDLPAVDYTLPPTSQSRKIVLVDGPSGKGGAVIR
                     MGIRAYDIHTDEKYAGSLASTILSSGIESRLGEYVRAKKGYVYGVFGIFQPGRHAGSF
                     TGNTETKLPTTADTIEAMFKVFRDMRDADVTADELAAAKRRVAGGLVMTMQTIAEQAR
                     LRGDGILNGYPIDYYDRYAGHVMNVTADQIRAVMRKYVDPNAMAIVVVAPAAEVKDQL
                     QRLGEVQVVPMPAKREGGNATTQRAGEMLK"
BASE COUNT          605 a         1160 c         1064 g          684 t
ORIGIN      
        1 gcgcttgtct cgctccggga aacgccgcga gtaaggtgag cttctcgtca aacgaccgca
       61 acaggctatc agctgcgacc agatcccgct ctgcgatata gtcccagatg atttggtagt
      121 tacggagaga cgcgcgcgtt cggcggatct ggcccatgct caagtgccca ggtgacgcga
      181 cgcccgcgcg gcgtgacgct cggcgatcac gtcctccgcg gtgaactcag caaagtcgcc
      241 acggtcagct tcagccaatc ccgcatcgac atccacacga agatcggcaa gttcctcggc
      301 ggctttctct tcgtcagtct tcagacgagc cacagccgca ttgatcaaat cgtcggcgga
      361 atcatacaga cccgccttga cgcgctcctc gacgaatcgg gcgagcttgg gatcgagtga
      421 aatgttcatc tgttgcttct cgtgcagatg ttaccgcgcc aagtcatcct gctccaacaa
      481 ctttcggtca gtgcgaaggt cacttcaaca tctcccccgc ccgctgcgtt gtcgcgttgc
      541 cgccttcgcg tttcgccggc atcggcacca cctgcacttc gcctaagcgc tgtagttgat
      601 ccttcacttc cgccgcgggc gcgaccacga cgatcgccat tgcgttgggg tccacgtact
      661 ttcgcatcac cgctcggatc tggtccgccg tcacgttcat cacgtgaccg gcgtagcggt
      721 cgtaataatc gatggggtaa ccattcagga tgccgtcgcc gcgcagcctc gcctgctcgg
      781 cgatcgtctg catcgtcatg accagcccac cggcgacgcg ccgcttggcg gcggcgagtt
      841 cgtccgccgt tacgtcggcg tcgcgcatat ccctgaagac cttgaacatc gcctcgatcg
      901 tgtccgccgt cgtcggcagt ttcgtctcgg tatttcccgt gaacgacccc gcgtgacggc
      961 cgggttggaa gatgccgaaa acgccgtaga cgtagccctt cttcgcgcgg acgtattcgc
     1021 cgaggcgcga ctcgatgccg ctgctgagga ttgtgctggc gagcgacccg gcgtattttt
     1081 cgtccgtgtg gatgtcgtac gcgcggatgc ccatacggat gacggcgccg cccttgccgc
     1141 tggggccatc aacgagcacg atcttgcgcg actggctggt cggcggcagc gtgtaatcga
     1201 ccgccggcaa atctttcggc tgccaaccat cggtcaattg cttcgccagc gcttgcccgc
     1261 gctccaccgt cacgtcgccg ctgatgatca gcaccgcgtc gttagggtga tagatctcgg
     1321 cataaaactg cttgacgtca tcgagcgtga tgcgcgacaa gctggccggc gtggccgggt
     1381 gacccagcgg tgtgccgccg aagagcgcgc ggagcaggtc catgtcagcc acggtgctgg
     1441 gattctccag cgcggccgtc agcccgctca gtgactgctc cttgaccttc tcgaattcat
     1501 cagcgggaaa cgtcggcgac aacagcacgt cgcgcgagcg tgcgaacccg cggtcgagtt
     1561 cttcggtggt ggtcgatccg acgagccgcg tgatgtcgcc gtcgtcgcgc acctccagcg
     1621 aaatgccgtg cgattccagc tcttcgctga gctgatgctc ggtgagattg ccagcgccgc
     1681 ggcgcagcat gtgggccgtc agatcggcca agccttcctt gcccgaagga tcacagtgcg
     1741 acccccggcg gatcgccaga ttccagttca ccagcggcag gcgatggtcc ggcatcacga
     1801 cgacgcgcac gccgttgatc gtttgttcgg tccccttctg aaacacggcc gccgatcgcg
     1861 ggtccgccat cggcggctgc gcgggataac ccccggggaa ctgcacgacc cgcgcctgga
     1921 tcggctcggt agacggggcg accggcgcgt ctttcttgct caactccgcc accgccatcg
     1981 tcgcggcttt gcgcgcctcg atccccagcg ggtccggtac catccgcacc gtcgtgcagc
     2041 catcgggctg aagatatttc gcggcgatcg ccttgatgtc cgcggcagtg acggcgtcga
     2101 tcttcgccaa ctcgcggttc acgcgcgatg gatcgccgcc gaacatcgct tcttcaccca
     2161 gctgcgtcgc gagattttcc gcggtcaaac ggttgtgaat gacctcgacc cgccgcagcg
     2221 tgcgcgcctt ggcgacctct tcatcgctca caccgttggc gaccacatcg gcgacggcgg
     2281 acttcagcac ccgctccact tcccccgcgt ccttgcccgg catcacccgc ccgctgacgc
     2341 cgaagatgcc ggcgtcttcg agattaaaga agatcgtctc cgcgctgtcg cacagcggat
     2401 gttcgctggt gaccagcagc cgctccagcc ggctcgacgc gccttcaccg aggatcgccg
     2461 acagcaccga cagcgcgtaa ttgtcgtccg actgataatc cggcgtgtgc cagcccgcga
     2521 cgatctgcgt cagcggcacg cgatcgggaa tcgtcgcatt gcgcggctgc gtctgcggcg
     2581 gctcgggctc ggcgcgccgc ggcacatccg cccccttggg aatccacccg aaatatttct
     2641 gcaccatcgc cttcgccgca tcgacatcaa tatcgccggc gacgaccagc accgcgttgt
     2701 tcggcacata gaaggtgttg aagaactcct gcaactcgct cgacggcgcc gccgccagct
     2761 gctgcatgtt accgatcggc gtccagcgat agctgtgcgt cgtgaacgcc gtcttcagat
     2821 agtcctcgta taaattgccg tacggccgat tctgcttgat gccccactcc tcggccacca
     2881 ctttgcgctc ggtgcgataa atctcatcgt tcaccttgaa gctgctcatg cgatcggctt
     2941 ccaggtacag caccatctcc aggttttccg ccggcacggt gttcacgtac gtcgtctgat
     3001 cgaagctggt gaacgcgttg gaatagccgc cgacctcgtt gacgagcttc atgtgctgct
     3061 gcggcgcgac atgagcggag ccgcggaaca tcatgtgctc gaacatgtga gcgaacccct
     3121 gccggtccgg ccgctcatcg cgcgagccga cgtgatagag cacgcgcacg tgaacgacgg
     3181 gggcttgatg cagcggcgcg tagatcacgc gcaagccgtt gggcagggtt tgctcctgat
     3241 actgaatcgg ttcggcttgt tgagcggtcg cagaacgcag cggcaggcag agcagaagca
     3301 gcagcgtgag gacgagcgag cgtgaacgat tcatgcgggg ttctccaagc gggttgtcga
     3361 ttcgcaacga tgatatacgc cgcgggcgcc gatgttacgg ctggagcggc tgaatgcgag
     3421 agtgatgcgc caagacgcca aaggaagagg attggacggc aaaatcgtag cacggacgtc
     3481 tcgcccgtgt cggagcacct tggcacgggc gag
//
LOCUS       DATIMH010000340         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1519546, whole genome shotgun sequence.
ACCESSION   DATIMH010000340 DATIMH010000000
VERSION     DATIMH010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519546"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VLI90_05845"
     CDS             <1..839
                     /locus_tag="VLI90_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13760.1"
                     /translation="FETPFRFYADRDHPLTSFGFHAYVAGTRPVSDLPQKQDLYLRNY
                     VPLIIDHTPANVPAQWWPARLRETFTLDSSAPFAVLVLIALVGLLTAPRLQWAVLGSL
                     PLFVILYACYVFFLPHYTIIVAPAIIVAIVIGLRALPQLAPLRAQRMVSAWLMIMTIG
                     LTVAALPQWDATANDQMFASPMIGSVNEALASLGGQRVVVLFRYDPQRNVHEEPVYNA
                     DVAWPDDAQIIRAHDRGIENTRIYRYYAEHQPDRVFYRFDEATKSLQRLGSATALAAQ
                     SK"
     gene            899..1498
                     /locus_tag="VLI90_05850"
     CDS             899..1498
                     /locus_tag="VLI90_05850"
                     /inference="COORDINATES: protein motif:HMM:NF016265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HSV13761.1"
                     /translation="MAENRERIEQFRKMAEADPTNEIGHFSLGRELFSAGQYDAAVQS
                     LRRVIELNPNISKAYQLVGEALLKQNQRDEAIKVLTDGVRIADSRGEVLPRKEMIQLL
                     TDAGAPVPELAVKPGAKLEVGEGDVLCKRCGRINKKLPRPPFKNPFGQQIFENTCADC
                     WREAIGLGTKVINELRLPMADPQAQKIWDQHIREFLNLG"
     gene            1637..>2780
                     /locus_tag="VLI90_05855"
     CDS             1637..>2780
                     /locus_tag="VLI90_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13762.1"
                     /translation="MVLGLALWAWVQEGDAQRNTPWPLLAVASALLLWQPFRRALTSS
                     SRWLTQRTSARRGITTIVVATVAGIYLFATARLQQRPFVPIYHDEFMYLLQARLLASG
                     RLWMPQHPLADFFDTFYVLGRPVYAPLAWPGISLFYVPGIWLAVPPWVTSLLLAACTT
                     GLVYRLVAQLMGDGFGLLAPALVLSLDLLRTYSILAFAHIGLLMLMIIAALALMAWTR
                     RGEIFWALCFGAAAGWACITRPVDALCFLAPMLVALLWQRPVARHGWRVAVRCAIAAS
                     VAALPFLALQLIFDRGVTGHSLKTPYALYCERDLPGVTFGFPKPDPHAMPASIVPQKQ
                     YLYSDWIVQLVRDHQPDRFISDWIHNRVPVALRMVTPHPLLLIALPF"
BASE COUNT          468 a          939 c          817 g          556 t
ORIGIN      
        1 tcttcgaaac gccgtttcgt ttctacgcgg atcgcgatca cccgctgacg tcattcggat
       61 tccatgcgta cgtcgccggc acccggccgg tctccgatct gccgcagaaa caggacctgt
      121 atctgcgcaa ctacgtgccg ctcatcatcg accacacgcc ggcgaatgtg cccgcgcaat
      181 ggtggcctgc gcgattgcgg gagacgttca cgctggattc atccgcgcca ttcgcggtgc
      241 tcgtgctgat tgcattggtc ggcctgctaa ccgcccctcg cctgcaatgg gccgtcctcg
      301 gctccctacc gcttttcgtc atcctgtatg cgtgctacgt ctttttcctg ccgcattaca
      361 ccatcatcgt cgcgccggcg ataatcgttg ccatcgtcat cggcctccgc gcactaccgc
      421 aactagcgcc gctccgcgcg cagaggatgg tgtcagcatg gctcatgatc atgaccatcg
      481 gcctcaccgt cgccgccttg ccgcaatggg atgcaaccgc gaacgatcag atgttcgcct
      541 caccgatgat tggaagcgtg aacgaagcgc tggcatcgct gggcggtcag cgcgtcgtcg
      601 tgttgttccg ttacgacccg cagcgaaacg tgcacgaaga accggtgtac aacgccgacg
      661 tcgcttggcc cgatgatgcg caaatcatcc gagcgcatga tcgcggcatt gagaacacgc
      721 gcatctatcg ctactacgcg gagcaccagc ccgatcgtgt cttctatcga ttcgatgaag
      781 ccacaaaatc gctccagagg ctaggcagcg ccaccgcgtt ggccgctcag tcgaagtaat
      841 cgcccgcccc gctataatcc cccggtactt agttgagccc agtaaaggag cttgtcacat
      901 ggcggagaat cgtgagcgga tcgagcagtt tcgcaagatg gccgaggccg atccgacaaa
      961 tgagatcggc cacttttcgc tcggccgcga gttgttcagc gccggtcagt acgacgcggc
     1021 ggtgcaatcg ctgcggcgcg tgattgagct gaatccgaac atcagcaagg cgtatcagct
     1081 ggtcggtgaa gcgctgctga agcagaacca gcgcgacgag gcgatcaagg tgttgaccga
     1141 cggcgtgcgc atcgccgact cgcgcggcga agtgttgccg cgcaaagaga tgatccaact
     1201 gctcaccgac gccggcgccc ccgtgccgga attggccgtc aaacccggcg cgaagctgga
     1261 ggtcggcgaa ggcgacgtcc tgtgcaagcg ctgcggccgg atcaacaaga agctcccgcg
     1321 cccgccgttc aaaaacccgt tcggccaaca gatcttcgaa aacacctgcg ccgactgctg
     1381 gcgcgaagcg atcggcctgg gcacgaaggt gatcaacgag cttcgcctgc cgatggccga
     1441 cccgcaggcg cagaaaatct gggaccagca catccgcgag ttcctgaacc tcgggtaacg
     1501 ccagcgcgcg cccccctccc gccatcgttt tggcgcgcat cgcacgtacg tggaacggat
     1561 ggaggtgctt tcggaatcat ccccgcccca cactcgccga cagccgctga tcgcacgcgc
     1621 tgcagcgctg tcagcgatgg tgctcggcct cgcgctgtgg gcctgggtcc aagaaggcga
     1681 cgcccaacgc aacacgccct ggccattact cgcggttgcg tcggcgctgc tgctctggca
     1741 gccctttcgc cgcgccctta catcctcctc acggtggctc actcaacgca cctcagcgcg
     1801 gcgcggaatc accacaatcg tcgtcgccac agtcgccggc atttatctct tcgccaccgc
     1861 gcggctgcag caacgcccgt tcgtgccgat ctatcacgac gagttcatgt acctgctgca
     1921 agcacggctg ctggccagcg gccggctttg gatgccgcag catccgctgg cggatttctt
     1981 cgacacattc tacgttctcg ggcgccccgt ttatgcgccg ctggcgtggc cggggatctc
     2041 gctgttttac gtcccaggca tctggcttgc cgttccgccg tgggtcacat cactgctgct
     2101 ggccgcctgc accacagggt tggtctatcg actcgtcgcc caattgatgg gcgatggttt
     2161 tggtcttctc gcgccggccc tggtgctgtc gctcgatctg ttgcgcacat actcgatcct
     2221 cgcgttcgcg cacattgggc tgttgatgct aatgatcatc gccgcgctgg cgttgatggc
     2281 ctggacacgc cgcggcgaaa tcttctgggc gctctgcttc ggcgccgcgg caggctgggc
     2341 atgcattacc cggccggtgg atgctctgtg tttcctcgcg ccgatgctgg tcgcgctgct
     2401 gtggcagcgg ccggtcgcgc gccacggctg gcgcgtcgct gtccgctgcg ccatcgccgc
     2461 ctcagtggcg gctctcccat ttctggccct ccaattgatc ttcgatcgag gcgtgaccgg
     2521 gcattccctc aagacgccct acgccttgta ctgcgagcgc gatctgcccg gcgtgacctt
     2581 cggatttccc aagcctgatc cccacgccat gccggcctca atcgtgccgc agaaacagta
     2641 cctgtattcc gactggatcg tgcaactggt gcgggatcac cagcccgacc ggttcatcag
     2701 cgattggatt cataaccgcg tgccggttgc actgcgaatg gtgacgccgc acccgttgct
     2761 gctgatcgcg ctgccgttcg
//
LOCUS       DATIMH010000341         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2384712, whole genome shotgun sequence.
ACCESSION   DATIMH010000341 DATIMH010000000
VERSION     DATIMH010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2384712"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1180
                     /locus_tag="VLI90_05860"
     CDS             158..1180
                     /locus_tag="VLI90_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HSV13763.1"
                     /translation="MKRSMMFAALFFAFTLATSASAAELNLFAWSEYVPQEVIDGFTK
                     ETGVKVNYETYASNEEMLSKLFAGASNYDVIQPSEYTVEALIRLKKLAPIDWSRVPNI
                     KNIDPQYLHLGHDPQQQFSIPWMTGTVGIVVNTNKVKDPIRGYKDVFQDKYKNRIVVV
                     NDNRELVSWALDTLGLPINDITPANLHKARPLLAKWIKLIKVFDSDSPKTALINGDVD
                     LGIVWSGEAALCFQQDKKFTYVLPIEGVHMFVDNLAIPASAAHKDDAMRFMNYCLRPE
                     VSKLISDKFPYTNPNAEARKLLTPEQLANPASYPKAPPKMEIFHDIGKASGEIDRLVT
                     DLKSAQ"
     gene            1250..2284
                     /locus_tag="VLI90_05865"
     CDS             1250..2284
                     /locus_tag="VLI90_05865"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV13764.1"
                     /translation="MPNSATTQSMQRPGIPATFLMLPILLWLLLLVVAPTVILVVYSF
                     CDRDEMGQVVFSFTLDNYRRIFDPDLTPVFAKAAGAGAIGAVLLAIARRTFAGARIGF
                     VLAFSGYLSYAIRHLEEDQGTYLRIFWRSTELAAFTAILCLLIGYPVAYFVGRASPRW
                     RNRLLAAIMIPFWTSFLIRTYAWITILNENGLLNTTLRSLHLTALIPASGGMLYTPTA
                     VVIGLVYAYLPFMILPIYGSVEKLDDALIEASLDLGAGPLRTFTNVILPLTRPGIVAG
                     IMLVFVPAIGMFAVTDLLGGARVPMIGNVIQNQFGQARDWPFGSALGVTLMAMFGLVF
                     LVTGRGAAER"
     gene            2347..3414
                     /locus_tag="VLI90_05870"
     CDS             2347..3414
                     /locus_tag="VLI90_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV13765.1"
                     /translation="MVSLRHLTKRFAAFTAVDDVSLDIRAGEFLTLLGPSGCGKTTLL
                     RMIAGFERPDAGSIFLDDRDVTPLPPYRRDVNQVFQSYALFPHLSVRENIAFGLRMRR
                     LPRATIETKVAAAVDWVSLAGMESRKPHQLSGGQRQRVALARALVCEPKVLLLDEPLA
                     ALDAKLRRSMQVELKRLQRRVGITFVFVTHDQEEAMTMSDRIAVMNHGRIEQLGDVAD
                     VYHRPCTAFVAGFLGHANLLPARVVARETDGTRIALADGSLLIASADATTEDMLISIR
                     PEKLTIAGTRPPAENVLEAEVKEAIFKGAFTQTLLRTAGGVELTALTANAANESPRQG
                     ERVFCQVHRDNIVLLPLLSGR"
     gene            complement(3477..>3716)
                     /locus_tag="VLI90_05875"
     CDS             complement(3477..>3716)
                     /locus_tag="VLI90_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13766.1"
                     /translation="AGRPVRQPAAGPDFLSVRHDRNASWPDDTNAANLTGYDATVGIQ
                     NARSRGNVEFCDGHVDYITREYIHTPNWWHWDPPR"
BASE COUNT          712 a         1273 c         1042 g          689 t
ORIGIN      
        1 gatcaaagtc ttcggcatgg cgaaggtcgg attgaacccg atgctgaacg cgatcagcgc
       61 gatcttcatc gccggcaccg cgcttttgat cggcgtcgcg gaatatttgc ggtcgccgcg
      121 ccaagcagat gcagcgccaa catgacaggg gagacgaatg aaacgctcga tgatgttcgc
      181 cgcgctgttt ttcgcattca cactcgctac atccgcgtcc gccgcggagc tgaacctctt
      241 cgcctggtcc gaatacgtcc cgcaggaagt catcgacggc ttcaccaaag agaccggcgt
      301 gaaggtgaac tacgaaacct acgcctccaa cgaagagatg ctcagcaaac tcttcgccgg
      361 cgcgagcaac tatgacgtca tccagccatc cgaatacacc gtcgaagcgc tcatccgcct
      421 gaaaaaactc gcgccgatcg actggtcgag agtccccaac atcaagaaca tcgacccgca
      481 atacctgcac ctcggccacg acccgcagca gcagttcagc atcccgtgga tgaccggcac
      541 cgtcggcatc gtcgtcaaca ccaacaaagt caaagacccc atccgcgggt acaaagacgt
      601 cttccaggac aagtacaaga accgcatcgt cgtcgtgaac gacaaccgcg agctggtctc
      661 ctgggcgctc gacacgctcg gcctgccgat caacgacatc acccccgcca acttgcacaa
      721 ggcccgcccg ctgctggcca agtggattaa gctaatcaag gtgtttgatt ccgacagccc
      781 caagaccgcg ttgatcaacg gcgacgtgga cctcggcatc gtctggagcg gcgaagcggc
      841 gctgtgcttt cagcaggaca agaagttcac gtacgtcctg ccgatcgaag gcgtgcacat
      901 gttcgtcgac aacctggcca tccccgcgtc ggccgcccat aaggacgatg cgatgcgttt
      961 tatgaactat tgtttgcgtc ccgaggtgag caagctgatc tcggataagt tcccgtacac
     1021 caatcccaac gccgaggcgc gcaagctgct cacgcccgag cagctggcca atcccgcgag
     1081 ctacccgaag gcgccgccga agatggagat cttccacgac atcggcaaag cctcgggtga
     1141 gatcgaccgg ctggtgactg atttaaagag tgcgcaatag cgcaataagg tcgaaagcca
     1201 ccaccaaagc ccatgtctcc gccgtgggaa ctcctcaaga acaatccaga tgccgaattc
     1261 cgccacgacc caatccatgc agcgccccgg catccccgca acatttctga tgctcccgat
     1321 cctcctatgg ctcctcctcc tggtagtcgc tcccacggtc atcctcgtcg tttacagctt
     1381 ctgcgatcgc gatgagatgg gccaagttgt cttctcgttc acgctcgaca actaccgccg
     1441 gatctttgat cccgacctga cgccggtctt cgccaaagcc gccggcgccg gcgcgatcgg
     1501 cgctgttctc ctcgccatcg cgcggcgaac gttcgccggt gcccgcatcg gtttcgtcct
     1561 cgccttcagc ggttacctct cttacgccat ccggcatctg gaagaagacc aaggcaccta
     1621 tctccgcatc ttctggcgtt ccaccgagct ggccgcgttc accgctatcc tctgccttct
     1681 gatcggctac cccgtcgcgt acttcgtcgg ccgtgcgtcg ccgcgctggc gcaaccgcct
     1741 gctggccgcc atcatgatcc cgttctggac cagcttcctc atccgcacct acgcgtggat
     1801 caccatcctg aacgagaacg gtctgctcaa caccaccctg cggtcgctgc acctgaccgc
     1861 cctcatcccc gcatcgggcg gcatgctcta cacgcccacc gccgtcgtca tcggcctggt
     1921 ctacgcatac ctgccgttca tgatcctgcc gatctacggc agcgtcgaaa agctcgacga
     1981 cgcgctcatc gaagcgtcac tggatctcgg cgctggtccg ctgcgaacgt tcacgaacgt
     2041 gatcctcccg ctgaccaggc ccggcatcgt cgccgggatc atgctcgtct tcgtccccgc
     2101 catcggcatg ttcgccgtca ccgatttgct cggcggcgca agggtgccga tgatcggcaa
     2161 tgtcatccaa aaccagtttg gccaggccag agactggcca ttcggctccg cgcttggcgt
     2221 gacgctgatg gcgatgttcg gactcgtgtt tctggtgacg ggaagggggg cagcggaacg
     2281 atgaacgatg aacgatgagc ggaaaacact catcgttcat cgctcatcgt tcatcgttcg
     2341 ccaactatgg tttccctccg ccacctaacc aagcgcttcg ccgccttcac cgcggtcgat
     2401 gatgtctcgc tcgacattcg tgccggtgaa ttcctcacgc tgctcggccc atcgggctgc
     2461 gggaagacca cgctgctgcg catgatcgcg ggcttcgaac gccccgatgc cggctcgatc
     2521 tttctggatg accgcgacgt cacgccgctg ccgccctacc gccgcgatgt gaaccaggtt
     2581 tttcagagct acgccctgtt cccgcacctg tcggtgcgcg aaaatatcgc gttcggcctg
     2641 cgcatgcggc ggttgccgcg cgcgacgatc gaaaccaaag tcgccgccgc cgtcgattgg
     2701 gtctcgctcg ccggaatgga atcgcgcaag ccacaccaac tctctggcgg acaacgccag
     2761 cgcgtcgccc tcgctcgcgc gctcgtctgc gagccaaaag tgctgctgct ggatgaacct
     2821 ctcgccgccc tcgacgccaa gctgcgccgc tcgatgcagg tcgagctcaa gcgtctgcag
     2881 cgccgtgtcg gcatcacgtt cgtcttcgtc acgcatgatc aggaagaagc gatgacgatg
     2941 tcggatcgaa tcgcggtgat gaaccacggc cggatcgaac agcttggcga cgtcgccgac
     3001 gtctatcacc gcccctgcac cgcattcgtc gccggatttc tcggccacgc gaatctgctg
     3061 cccgcgcgcg tcgttgcccg cgaaaccgat ggcacgcgca tcgccctcgc cgacggaagc
     3121 ctcctcatcg caagcgccga cgcgaccacc gaagacatgc tgatctcgat ccgtcccgaa
     3181 aagctgacca tcgcggggac gcgcccgccg gccgaaaatg tattggaagc ggaagtgaaa
     3241 gaagcgatct tcaaaggcgc gttcacccaa acgctgctgc gcacggccgg cggcgtcgaa
     3301 ctcaccgcgc tgacggccaa tgctgcaaac gaatcgcccc gccagggcga gcgcgttttc
     3361 tgccaggttc accgtgataa catcgtgctg ctgcccctgc ttagcggccg ctaacgccgc
     3421 aagcggcggt gggcgctgcg cgagcgcaca tcgatgacag ctctttcggc tcggccctag
     3481 cgtggcggat cccagtgcca ccagttcggc gtgtggatgt actcgcgcgt gatgtaatcg
     3541 acgtgcccgt cgcagaactc gacgttgccg cgggagcggg cgttctggat gccgaccgtc
     3601 gcgtcatagc cggtgagatt ggcggcattg gtgtcatctg gccagctggc gttgcgatcg
     3661 tggcggacgg agaggaagtc cggcccggca gcgggctgcc gaactgggcg cccagc
//
LOCUS       DATIMH010000342         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2964235, whole genome shotgun sequence.
ACCESSION   DATIMH010000342 DATIMH010000000
VERSION     DATIMH010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2964235"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VLI90_05880"
     CDS             complement(<1..879)
                     /locus_tag="VLI90_05880"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSV13767.1"
                     /translation="MPTSFVDMRVDGSTASATLSRSGVDAELLDLIEPIGSRPSSPLA
                     ASTVIVMAHPDDEVVSLGSRLTRLSQATLVHTTDGAPPNGLDARAHGFTNAADYAAAR
                     RHEMERAMALSGIPLDHLRELNCPDQGASHMLTKLAIECAALFRELHARRIITHPYEG
                     GHPDHDATAFAVHAAAAVGASAQSRPLLVEMTSYHLGPGGIETGKFLHPSATPFSEHL
                     SRDECATKRAMLACFNSQRETLQYFTDEVEQFRMAPTYDFLSAPHPGLLFYEKFNWGM
                     TGSQWRQLARAALHQLH"
     gene            complement(986..1771)
                     /locus_tag="VLI90_05885"
     CDS             complement(986..1771)
                     /locus_tag="VLI90_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSV13768.1"
                     /translation="MWAIIPAAGMGTRIQPLAFSKELLPVGSRLDGRTERPLAVSEYL
                     VDRMILAGATRICFVISPAKSDIVQYYGEGVGAAKFCYVVQQRPAGLCDSIFHALPFI
                     GTGEDVVVGLPDTVWFPEDGLCALPDGKLSFLLFPVSHPEFFDAVVTNEHGEVVEVQV
                     KRPDAESHWVWGAFKIPQSVLMDLHEIWREREMHDEYIGTLVNEYLRRGGHAVGVPAG
                     ESYVDVGTLHGYREAIHLLEQRPPRPVRVAAASARAMEGANTP"
     gene            complement(1791..2921)
                     /locus_tag="VLI90_05890"
     CDS             complement(1791..2921)
                     /locus_tag="VLI90_05890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV13769.1"
                     /translation="MKIVIFGLTVTSSWGNGHATLWRGLCRALARRGHSIVFFEKDVP
                     YYASERDLEILTGCWLHMYRSWNEALPVAIRESSNADVAMVTSFCPDGAAASDVVLSS
                     SAQLRVFYDMDTPVTLEKLRGGEQVFYLPSRGLGEFDLVLSYTGGAKLENELRQRLGA
                     RRVAPLYGSVDPELHHPVPSVDKFRADLSYMGTYAADRQAGVEELLLEPARRLPERQF
                     SVGGAQYPPEVHWTENVRFTQHVAPPQHAAFYCSSRLTLNVTRKVMADMGYCPSGRFF
                     EAAACGTPILSDWWEGLDQFYKPGSEILIAHNTDDAVGAVELSDRELSRLSRAARERT
                     MAEHTAERRAIEFENAVESARQACSSGSERARRDSMSAPLAG"
     gene            complement(2978..3199)
                     /locus_tag="VLI90_05895"
     CDS             complement(2978..3199)
                     /locus_tag="VLI90_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13770.1"
                     /translation="MHVKLGPYHVWDSPNSAGVVIDAVRRAKLALDRGIGGALSSPSS
                     YFTKSPPEQYTDAESRARTDAFIQGEADA"
BASE COUNT          775 a         1295 c         1218 g          718 t
ORIGIN      
        1 gtgcaactga tgaagcgccg cgcgtgcaag ctgccgccat tggcttccag tcatgcccca
       61 attgaatttt tcataaaaca gcagccccgg atgcggcgcg ctcaggaagt cgtaggtcgg
      121 cgccattcgg aattgttcca cttcatcggt aaaatattgc aacgtctccc gctggctgtt
      181 gaagcacgcc agcatggcgc gtttcgtcgc gcactcgtcg cgggacaaat gttcggaaaa
      241 cggagtcgcc gatggatgca aaaacttccc cgtctcaatt cctcccggac cgaggtggta
      301 cgaagtcatc tccaccagga gaggccggct ctgcgcgctc gcaccaacag cagcggcagc
      361 atgaaccgcg aacgccgtcg catcatgatc gggatgcccg ccttcatacg ggtgtgtgat
      421 gatgcgcctc gcgtgcagct cacgaaacag cgctgcacac tcgattgcca gcttcgtcaa
      481 catgtgcgaa gcgccctgat cgggacagtt cagttcgcgc agatgatcca gcgggatccc
      541 agacaaggcc atcgctcgtt ccatctcgtg ccggcgagcg gccgcataat ctgccgcgtt
      601 tgtaaaaccg tgtgcgcggg catctaaacc attcggcggc gcgccgtcgg tcgtgtggac
      661 cagcgtggcc tgggacaacc tcgttaaacg tgagccaagg ctgacgacct catcatccgg
      721 atgcgccatc acgatgaccg tgctcgcggc cagcggtgat gatggccgtg agccgatcgg
      781 ctcgatcaga tcgagcagtt ccgcgtccac acctgagcgt gatagggtcg ctgaagctgt
      841 tgagccatca acacgcatat cgacgaacga tgtcggcaca gaagtcggca tactcattga
      901 tgcacctggg tgggctggat gcggcgctcg agattgtcgc gcgcgtatgg cgctggtgcg
      961 ggagttgcca tctcctcaga agcggtcatg gcgtgttcgc tccctccatc gctcgtgccg
     1021 atgccgcggc gacgcggaca ggccggggtg ggcgctgctc cagcaaatga atcgcctcgc
     1081 ggtagccgtg caacgtcccc acgtccacgt aagattctcc cgcgggcaca cccaccgcgt
     1141 ggccgccgcg acgcaggtat tcgttgacca gcgtgccgat gtactcatcg tgcatctcgc
     1201 gctcgcgcca aatctcgtgc aggtccatca gcacgctctg cggaattttg aatgcgcccc
     1261 acacccagtg cgactcggca tccggccgct tcacctggac ctcgacgacc tcgccatgct
     1321 cgttcgtgac caccgcgtcg aagaactccg ggtgcgacac gggaaacagg aggaatgaaa
     1381 gtttgccgtc cggcagcgcg cacaggccgt cctccggaaa ccaaaccgtg tccggcaggc
     1441 cgacgacgac atcttctcct gtgccgatga acggaagcgc gtggaagatc gagtcgcaca
     1501 atcccgccgg ccgctgctgc acgacatagc agaacttcgc cgcgccgacc ccttcgccgt
     1561 agtactgaac gatgtccgat tttgccggcg agatgacgaa gcaaatccgg gtggcgccgg
     1621 cgaggatcat gcgatcgacc agatattcgc tgacggccaa cggacgttcg gttcggccat
     1681 cgagacggct gccgaccggc aacaactcct tggaaaatgc caacggctgg atgcgtgttc
     1741 ccatgcccgc cgccggaatg atcgcccaca taaggcactc ctcagatgct ttagccggcc
     1801 aacggcgccg acatcgaatc gcgccgcgcc cgctcgcttc cgctgctgca agcttgcctg
     1861 gccgattcaa ccgcattctc aaattcgatc gcccgccgct cggcggtgtg ctccgccatc
     1921 gtgcgctcgc gcgcggcgcg actcagccgc gacagttccc ggtcggacaa ctcaacggcg
     1981 ccaacggcat cgtccgtgtt gtgcgcgatg aggatctccg agcccggctt gtaaaactga
     2041 tccagacctt cccaccagtc gctgagaatc ggcgtcccgc aggcggcggc ctcgaagaat
     2101 cggcccgacg ggcagtagcc catgtcggcc atcaccttgc gagtcacgtt caacgtcagt
     2161 cgcgacgagc agtaaaacgc cgcgtgctgc ggcggggcga cgtgctgggt aaatcggacg
     2221 ttctcggtcc agtggacctc cggcgggtac tgagcgccgc cgaccgaaaa ctgccgctcc
     2281 ggcaaccgcc gtgcgggctc gagtaaaagt tcctccacgc ccgcctgacg atcagcggcg
     2341 taggtcccca tgtaagacaa gtccgcgcga aatttgtcga cggatggcac cggatgatgc
     2401 aattccggat cgacgctgcc atacagcggc gcgacccgcc gcgcgcccaa ccgctggcgc
     2461 aattcattct caagctttgc gccaccggtg tagctcagca ccaggtcgaa ctcgccgagc
     2521 ccgcgcgatg gcaggtaaaa gacctgctca ccgccgcgca acttttccag cgtgaccggc
     2581 gtgtccatgt cataaaacac ccgcaactga gccgacgacg agagcaccac gtccgacgcc
     2641 gccgcgccat cggggcagaa cgatgtcacc atcgcaacat cggcgttgct gctctcgcgg
     2701 atcgccaccg gtagcgcttc gttccagctg cgatacatgt ggagccagca accggtgaga
     2761 atctccaagt cgcgctcgct ggcgtagtag ggcacgtcct tctcgaagaa gacgatgctg
     2821 tggccgcggc gcgccagcgc gcggcagagg ccccgccaca gtgtcgcgtg accgtttccc
     2881 caggaactgg tcaccgtcag cccgaatatc acgatcttca ttgctcacgt cctcttgtgt
     2941 gcgccgtcgc gcgcggcgcc cgatatttgc cggagcctca agcgtcagcc tcgccctgga
     3001 tgaacgcgtc ggtgcgggcg cgcgactcgg cgtccgtgta ctgctccggc ggggacttcg
     3061 taaagtagct cgaggggctc gacagcgcgc cgcctatgcc gcgatctaac gcaagcttgg
     3121 cgcggcgaac cgcgtcgatc acgacgcccg cgctatttgg cgaatcccat acgtgatacg
     3181 ggccgagctt cacgtgcatc aatcccggca ccgatgtgcc cggttcagcg tttcgatata
     3241 agtgaacacc ctgaataagt gacgatgcgc aattgccgcc agcgacgacg gcaacgcgaa
     3301 cagtggtagg gctgctcatg tgaaagccgc tcctgcgcag ataagttcgg tactccgtca
     3361 ccgatgggtc cgccgatgcg tgtccgccac gatcgaccgg agatgctggc ccgcgtccac
     3421 aagcatcccg caaaaacagc gcgcgttccg agcagtctta tccctcgctt gtggttcgga
     3481 gcgaataacc acgtcttggg gcggcgtgaa gtaaagacga cccgcgcgaa ttggccaacc
     3541 aagagttcct tctgtcgcgg cactcgccgg tgggtcgggg ggcgaccaag ctgatgcacg
     3601 cgggcgccgg agctgcggta gcgctgtgca gctcacgcaa gcggccgacg atgacccggc
     3661 aacacccagg gatttggacc gaaacaaatt ctgtcgtcgc aggggaaatg gtgtgatctt
     3721 ggtttcgcag gaccgtcacc gccgtctgcg gttgagtgcc agacgaggtc ggtagagttg
     3781 ctgcagcggg aggggcgcaa aaaagcaatc caccacggat cgttggtgac gagcgtggtg
     3841 ggtttcgtta cggtgagggg gggacacggc tagaacttga acccttcgac tcgctccgct
     3901 cgctcaggac gttcgactcg cgtggagtca atcggcctgc cgtgagcgag cgcagcgagt
     3961 cgtacggtct gtctgggaca gccttcgaac ctttttcaag gtcaac
//
LOCUS       DATIMH010000343         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5563991, whole genome shotgun sequence.
ACCESSION   DATIMH010000343 DATIMH010000000
VERSION     DATIMH010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5563991"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VLI90_05900"
     CDS             complement(<1..238)
                     /locus_tag="VLI90_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13771.1"
                     /translation="MVDAHDHLIHASGADIPATARVSTVQASKLAGLRKPSAEDASTG
                     CHADAGTVLYYVDRPGLLKYSIERLSDAPDDLLMR"
     gene            complement(415..708)
                     /locus_tag="VLI90_05905"
     CDS             complement(415..708)
                     /locus_tag="VLI90_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13772.1"
                     /translation="MNRLAIGFIAALAISPFGVSLLYAQANPSASGSAPRGGPGGTSM
                     ISPTPGPATSPALPSANTPEVTVANKDKAKAAEAERKAQKKAKKAKKKAPATQ"
     gene            complement(772..>1549)
                     /locus_tag="VLI90_05910"
     CDS             complement(772..>1549)
                     /locus_tag="VLI90_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSV13773.1"
                     /translation="DLGRRLGRDYADLKALIAGDGVKIAATHLGAAPDYALSDITWLP
                     VIPNPGKIVCVGLNYQEHIAETGRDNTEQPSLFLRVAESQVGHRQAIVRPRESTHLDF
                     EAEIAIVIGLPGRRIAQADAWRYVAGYSCYNDGSVRDWQRHTTQWTAGKNFSRTGGFG
                     PWLVTADEIPPGTVLTLSSRLNGKEMQRATTDQMIFKIPKLIEYISAFTSLLPGDVIV
                     TGTPGGVGARRTPPVWMKPGDTIEIEVDKVGVLQNSIVDG"
BASE COUNT          269 a          489 c          483 g          308 t
ORIGIN      
        1 gacgcatcaa caggtcgtcg ggtgcgtcac tcagtcgctc aatggaatac ttgagcaaac
       61 cgggccgatc gacatagtac aaaacagttc cggcatcagc gtgacagccg gtggatgcgt
      121 cttcagcgga aggttttcgc aagccggcca gtttggaagc ctgaacggta cttacacgtg
      181 cagtggcggg gatgtcggca ccggacgcgt gtatgagatg atcgtgggca tccaccacgt
      241 gtcagcacgc ttcgacacca tcagtaccgg cgctcgcggt tgccaatcga gtggatacct
      301 cgccggtatt cggcgccagt aatcaatccg cgatttgcga tcatcaacca acaggcgtga
      361 gttatgcctg cgagtgaagc ccccctgcga tgataccggt gacgcgcgac atggctactg
      421 cgtcgccggc gccttcttct tcgctttctt cgccttcttt tgcgccttgc gctccgcctc
      481 cgccgccttg gccttgtcct tgttggccac ggtcacctcg ggggtgttgg cagacggcag
      541 cgccgggctc gtggccggac ccggcgtcgg gctgatcatc gacgtgcctc ccgggccgcc
      601 gcggggcgcg ctgcccgagg cactgggatt tgcctgggcg tacagcaggg aaaccccgaa
      661 tggggaaatc gcgagcgcag cgatgaagcc gattgcgagt ctgttcatgg ccagtccttc
      721 agtcctgttc tcgagtcgcc agtctgcagt ccgtgagggg ctgccgcggg ttcagccgtc
      781 cacaatgctg ttctgcagga cgccgacctt gtcgacttcg atctcgatgg tgtctcccgg
      841 cttcatccag accggcggcg tccgacgcgc accgacgccg cccggcgtgc cggtcacgat
      901 cacgtcgccg ggcagcaggc tcgtgaatgc cgagatgtat tcgatgagct tgggtatctt
      961 gaagatcatc tgatccgtgg tcgcgcgctg catctccttc ccgttgagac gggatgagag
     1021 cgtgagaaca gtgcccggcg gaatctcgtc cgcagtcacc agccacggac cgaagccgcc
     1081 cgtgcgcgag aagttcttgc ctgccgtcca ctgcgtggtg tgacgctgcc aatcgcgaac
     1141 cgagccgtcg ttgtagcagc tatagccggc gacgtaacgc cacgcatccg cctgcgcgat
     1201 ccgccgtccc ggcaagccga tgactatcgc gatctccgct tcgaaatcga gatgcgtcga
     1261 ttcgcgcgga cgaacgatgg cctgacggtg cccgacctgt gactctgcga cgcgcaggaa
     1321 caatgacggc tgctcggtat tgtcgcgtcc ggtctcggcg atgtgctcct ggtagttgag
     1381 acccacgcac acgatcttgc cagggttcgg aatcaccggg agccaggtga tatccgacag
     1441 cgcgtagtca ggtgcggcac ccagatgtgt cgccgcgatc ttcacaccat cacccgcaat
     1501 cagtgccttc agatctgcat agtcgcgacc gagtcggcgg ccgagatcg
//
LOCUS       DATIMH010000344         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3253675, whole genome shotgun sequence.
ACCESSION   DATIMH010000344 DATIMH010000000
VERSION     DATIMH010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3253675"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1160
                     /locus_tag="VLI90_05915"
     CDS             141..1160
                     /locus_tag="VLI90_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HSV13774.1"
                     /translation="MNTLILENPQQFRFAQTDEPDLTLAPGQALVRVRRVGICGTDWH
                     AYHGRQPFFTYPRILGHELGVVVEAVKDPASDLKPGDKCSVEPYMNCGVCIACRRGKT
                     NCCAKLNVIGVHTDGGMRERIVLPARKLHKSDKLTLEQLALVETLGIGGHAVDRAAIE
                     RDEWVLIIGAGPIGLSIIPFAKAAGARVIVTDVSESRLAFCQKQMGVQHMIDARGDVL
                     AELKRLTNEDLPTAVFDATGAPASMMKTFDCIAPGGRIIFVGLFQGDVTFNDPNFHRR
                     EISLLGSRNARASDFKRIIKMMEDGTVDTTPWITHRAACNQLVQTLPDWLKPDAGVIK
                     AMVEF"
     gene            complement(1292..1552)
                     /locus_tag="VLI90_05920"
     CDS             complement(1292..1552)
                     /locus_tag="VLI90_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13775.1"
                     /translation="MTCLDLQRKLHDKPFKPFRIWLVNNTTYDVTEPWMVTVGETSAV
                     IVTQSRKDEKGYELALDWRTVSISHMLEFSDLDVKERKRRPA"
     gene            complement(1549..>2492)
                     /locus_tag="VLI90_05925"
     CDS             complement(1549..>2492)
                     /locus_tag="VLI90_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13776.1"
                     /translation="KFGSDIIEYNPTNNAIHNGTNVHTPLVTHSISGLATNGVGIVNG
                     TDGYIYSVGSAGLQRIDPSNWAAPAVTLPGSVGGQGYGVNTLPNGKIVYSDGSNSSSV
                     WVYDPVALTNTLIYSASFLVDDIETGPAGEIALAGQSNSSITILNSGGGLIKQFNTAR
                     FPDGLAFGAGPFANSIYANNNDGSITRYDMSAGWTGTVTPVDIATTLAGHHGYGDLAS
                     VGPDCAFYISTFPNVNFGHGNDAGIGTNWDNGVTNGEASIVRVGSSGIGADGLPNSDC
                     VFYSLIETTIPEPGSAMILAIGMLPMLCRRRSHRMRL"
BASE COUNT          454 a          785 c          806 g          447 t
ORIGIN      
        1 tcccggcgac ctgcggtcag cagtaccggg agaggggacc agagcgcgcg attcctacat
       61 gcgtttaccc tgcctccgcc gcacggtcat cgacggatgc cgtgcagcct cgcgctcgca
      121 cgaatatgat ggcaagccga atgaacaccc tcatcctcga aaacccccag cagttccgtt
      181 tcgcgcagac cgatgaaccc gacctcacgc tcgcgccggg gcaggcgctg gtgcgcgtgc
      241 ggcgggtcgg catctgcggc accgactggc acgcgtacca cggccgccag ccgttcttca
      301 cttacccgcg catcctgggg cacgaactcg gcgttgtggt cgaagcggtg aaggatccgg
      361 cgtcggacct gaagccgggc gacaaatgtt ccgtcgagcc gtacatgaac tgcggcgtct
      421 gcatcgcctg ccggcgcggc aagaccaact gctgcgccaa gctcaatgtc atcggcgtgc
      481 acaccgacgg cggaatgcgc gagcgcatcg tcctgccggc gcgcaagctg cacaagtcgg
      541 acaagctcac gctggaacaa ctggcgctgg tggaaacgct cggcatcggc gggcatgccg
      601 tcgatcgcgc cgcgatcgag cgcgacgagt gggtgctgat catcggcgcc ggcccgatcg
      661 gcttgtcgat catcccgttc gccaaggccg ccggcgcgcg cgtcatcgtc accgacgtca
      721 gcgaatcgcg cctcgcgttt tgccaaaagc agatgggcgt gcagcacatg atcgacgccc
      781 gcggcgacgt gctggcggag ttgaaacgcc tgacgaacga agacctgccc accgccgtct
      841 tcgacgccac cggcgccccg gcgtcgatga tgaagacctt cgactgcatc gcccccggcg
      901 ggcggatcat cttcgtcggc ttgttccaag gcgacgtgac cttcaacgat cccaacttcc
      961 accgccggga gatttcgctg ctcgggtcgc gcaacgcgcg ggcgagcgac ttcaagcgca
     1021 tcatcaagat gatggaagac ggcacggtgg acaccacgcc gtggatcacc caccgcgcag
     1081 cgtgtaacca actggtgcag acgctacccg attggctcaa gccagacgcg ggcgtgatca
     1141 aggcgatggt ggagttctga tcgactccgt ggttgagagt cgcagccgcg cggcttcaga
     1201 ccgcggagcg ttcgacgttc caacggcgct ggtcacccaa aaagtccgcg ggctaaagcc
     1261 cggcggctgc gatgggcgcg gctcgcaacc atcaggcggg tcttcgcttt cgctccttca
     1321 cgtcgagatc ggaaaactcc agcatgtgcg agatggaaac cgtgcgccag tccagcgcca
     1381 gctcgtaccc cttctcgtct ttccgcgact gcgtgacgat caccgcgctg gtttcaccga
     1441 ccgtgaccat ccagggctcg gtgacgtcgt acgtcgtgtt gttgaccaac caaatccgaa
     1501 acggtttgaa tggcttatcg tgaagcttcc gttgcagatc taagcaggtc ataaccgcat
     1561 tctatgcgag cgtcgccgac agagcatcgg caacatgcca atcgccagaa tcatcgcgct
     1621 gcccggctcg ggaatcgtcg tctcgatcag cgaataaaac acgcagtcgc tgttgggcaa
     1681 accatcggcg ccgatgccgg aggaaccgac gcggacgatc gacgcttcgc cgttggtgac
     1741 gccgttgtcc cagttggtgc cgatgccggc gtcgttgccg tggccgaagt tcacgttggg
     1801 gaacgtgctg atgtagaacg cgcagtcggg accgacagag gcgaggtcgc cgtaaccgtg
     1861 atggccggcc aacgtggttg cgatatcaac cggcgtgacg gtgccggtcc agccggcgct
     1921 catgtcgtag cgcgtgatcg agccgtcgtt gttgttggcg tagatcgagt tggcgaacgg
     1981 gccggcgccg aaggcgagac cgtcgggaaa gcgcgcggtg ttgaactgct tgatcaagcc
     2041 cccgccgctg ttcaagatcg tgatgctgct gttgctctgg ccggcgaggg cgatttcacc
     2101 ggcggggccg gtttcgatgt cgtcgacgag gaacgacgcg gagtaaatca gcgtgttggt
     2161 cagcgcgacc ggatcgtaga cccagacgct gctggagttg ctgccgtcgg agtagacgat
     2221 cttgccgttg ggcaaagtgt tgacgccgta gccctgtcca cccacgctgc ccggcagcgt
     2281 caccgcgggc gcggcccagt tgctcggatc gatgcgctgc aaaccggcgc tgccgacgga
     2341 atagatatag ccgtcggtgc cattgacgat gccgacgcca ttcgttgcca acccgctgat
     2401 cgagtgcgtg accagcggcg tgtgaacgtt ggtgccgtta tgaatggcgt tgttcgtcgg
     2461 gttgtactcg atgatgtcgc tgccgaactt gg
//
LOCUS       DATIMH010000345         5790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1521748, whole genome shotgun sequence.
ACCESSION   DATIMH010000345 DATIMH010000000
VERSION     DATIMH010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521748"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VLI90_05930"
     CDS             complement(<1..336)
                     /locus_tag="VLI90_05930"
                     /inference="COORDINATES: protein motif:HMM:NF036305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13777.1"
                     /translation="MTATLHFTHLVRPSIARSAVEAELQVHQTVQQWVDECIDLCQPN
                     DVHICTGSAEEKQQLIERGIEEGIFIKLNQEKLPGCYLHRSNPNDVARTEQSTFICAP
                     SQDMAGPTNN"
     gene            665..1753
                     /locus_tag="VLI90_05935"
     CDS             665..1753
                     /locus_tag="VLI90_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSV13778.1"
                     /translation="MSDGTKSSTSTPAAATEAALRDSASRADAILNTTVDGIITIDER
                     GLIESFNPAAERLFGYTAAEVIGRNVSQLMPQPYQGEHDQYIRNYLTTGRPKIIGLGR
                     EVLGLRKDGATFPMHLAVSEVRLPGAPGRPRLFTGIVHDLSERKRLERELLEISDREQ
                     RRIGRDLHDGLGQQLAGIGFLSKSLENRLAAQDSPEAADARKIAELVAVAIAQARAMA
                     RGLHPVDLKASGLMSALEELAANVEGIFRVRCALDCPQPVLLSDNAVAVHAYRIAQEA
                     VNNALRHGKAKRIVISLERAGSAVTLKVHDDGSGLPATPIKNGGMGMQIMRYRAALIS
                     ASLSFTPAQPHGTVVSCTFIHPDQFAQR"
     gene            1765..2460
                     /locus_tag="VLI90_05940"
     CDS             1765..2460
                     /locus_tag="VLI90_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSV13779.1"
                     /translation="MSAKKAPQTEIKPAAAEKKKVMLVDDHPLVREGLGRLINDQADL
                     IVSGEADGPVRALELLKHSRPDLVALDLSLAGGDGLELCKQLHELYPELPVLIISMHD
                     ESLYAERALRAGASGYVMKQAPQEQVMQAIRRTMGGETWLSDKMSAKLLRSIRGARAG
                     ADVSPLERLTDRELEIFRMIGQGLSVKAIADALFLSPKTIEAHKEHIKQKLNLETSND
                     LLQYAIQARAQTG"
     gene            2566..2946
                     /locus_tag="VLI90_05945"
     CDS             2566..2946
                     /locus_tag="VLI90_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13780.1"
                     /translation="MRVLLHPPIEELELGRRAVRAKICPGCYQSASSERALDQLTPRS
                     CEPGCTIFTNLPAMHLAVANRLTVLADADKAAEQVVCETCHARPTSGEFCAAFSSRTC
                     PLSRYGREVIDLLQRMRDRHVRVS"
     gene            complement(3082..4650)
                     /locus_tag="VLI90_05950"
     CDS             complement(3082..4650)
                     /locus_tag="VLI90_05950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV13781.1"
                     /translation="MPARSEARLDSVTANAAPAPPPRRIPLAGLLLTLILIAAAALRI
                     YNLSALGYWTDEFCTLSTANGWGLVFLRTPMDQVVPPTQPVATRYDHARPRADILPAV
                     VREDAHPPLYILLVRAFEALFRDDSEAHVRAVGVVFSVAAVALLYFVARPFVGTSAAL
                     WACLLLAVTGPQIEFSQEAGRYMPLMTFTLAAAWALARMRQRATIGAAIALGAATLAM
                     ALTHYFAVGAAFAFGLYALLALGGRARMLAIAAMAIAGVVFMATWGPFMLRQHPNVDA
                     YYGWVQDHAGPGHAARVLLDLDRLPARFLAEPSPSGWSNFVTLVAGVLWLLLPIAFVR
                     RPNLRLFVLWIICAVGLVLCLDLARGTLQLRVLRYTLVATPAACVLIAAAVPRGRWAF
                     VPPALAVTFALLRLPAAYVPPWKYDFRTPVEIVAPRLRAGDALVLASTDPLLVAVSYT
                     AFQHYALPAMPAVVATLTKPADNPTLDRLRACGHVWIVWMTGRATPQQWLPGLRVEQS
                     GELTAESRIVEGRF"
     gene            complement(5024..>5790)
                     /locus_tag="VLI90_05955"
     CDS             complement(5024..>5790)
                     /locus_tag="VLI90_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSV13782.1"
                     /translation="SPGGLVFGHPDNMSTNIPGLYAMGEVSFAYHGANRLGANSLLSC
                     IFDGLFGGTCIKNYITDTAKTAADDVPAATFERFEKQEKDRQNWLVTNDGNENPYLLW
                     QEMGKWMTENCTVVRYNEKLELTLEKCQEWKDRFKRVKLSDTGMWTNQNLSFARATRD
                     MILESEAILKGALARNESRGAHYKPEHAERDDANFLKATIATYDAASDSAVISYAPVD
                     TSLVKPRARTYGKTADNPAAKAPAAAATPEPAPAGV"
BASE COUNT         1059 a         1832 c         1908 g          991 t
ORIGIN      
        1 gttgttcgtc ggcccggcca tgtcctgact gggcgcgcag atgaacgtgc tttgttcggt
       61 ccgggcgacg tcattgggat tgctgcggtg cagatagcag cccggcagct tctcctggtt
      121 gagtttgatg aagatgcctt cttcgatgcc gcgttcgatc agctgctgct tttcctcagc
      181 cgagccggtg caaatgtgaa cgtcgttggg ttggcatagg tcgatgcatt catccaccca
      241 ttgctgcacc gtttgatgca cctgcaactc agcctcaacg gcggagcgtg cgatggatgg
      301 gcgaaccaag tgcgtgaagt gcaacgtcgc tgtcatgaca agtcctcttt aggaagccgc
      361 attcccagtg agcgcggggc cggggcggcg gccgcattca cctctgaaga agtatcgggg
      421 cctcgaagga aggggactat taggtaccgc ccgtgttcga acgtaggggc tttccccgag
      481 cagggagtaa agaggcgaaa cggggcgcac ccacgttcga cgccggtgtc tgaggtactc
      541 cgaggacctg agtatcttcc gcgcagtacc cggatcttcg gagtacctca gacaccggct
      601 tcgcgatgcg cgcgattgct cgtttgtcga gcaacattca ctacaatcct cgcaggacga
      661 agcgatgtca gacggaacga aaagctcgac ctccaccccc gccgccgcga cagaggcggc
      721 cctgcgcgac agcgcatcgc gcgccgatgc catcctcaac accaccgttg atggcatcat
      781 cacgatcgac gaacgcggct tgatcgagtc cttcaacccc gcggccgagc ggttgttcgg
      841 ttacaccgcg gcggaggtga tcggccgcaa tgtcagccaa ctcatgcccc agccgtatca
      901 gggggagcac gatcaataca tccggaatta cctcacgacc ggccgtccga agatcatcgg
      961 gctcggtcga gaagtgttgg gacttcgcaa ggacggcgcg acgttcccga tgcacctggc
     1021 ggtcagcgaa gtgcggctgc cgggcgcgcc gggccggccg cggctgttca ctggaatcgt
     1081 gcacgacttg agcgagcgca aacggctgga gcgcgagctg ctggaaatca gcgatcgcga
     1141 gcagcgccgc atcggccgcg atctgcacga tggcctgggc caacaactcg cgggcattgg
     1201 attcttgagc aagtcgctgg agaaccgcct ggccgcgcag gattcaccgg aggcggccga
     1261 cgcgcgcaag atcgccgagc tggtcgccgt cgccatcgcg caggcccgcg ccatggcccg
     1321 cgggcttcac ccggtcgatc tgaaagccag cggattgatg tccgccctgg aagagttggc
     1381 ggcgaatgtg gaaggcattt tccgcgtgcg ctgcgcactg gattgcccgc agccggtgct
     1441 gttgtccgac aacgctgtgg cggtccacgc gtatcgcatt gcgcaggaag cggtgaacaa
     1501 cgcgctgcgt catggcaagg cgaagcggat cgtcatctcc cttgagcgcg ccggtagcgc
     1561 ggttaccctg aaggtgcacg acgacggcag cggactgccc gcgacgccga tcaaaaacgg
     1621 cggaatgggc atgcagatca tgcggtatcg agcggccttg atcagcgcat cgctgagctt
     1681 caccccggcc cagccgcacg gcacggtcgt cagctgcacg ttcattcatc ccgatcaatt
     1741 tgcgcaacgg taggagtgtc ttacatgtca gcgaagaaag cgccgcaaac cgaaatcaag
     1801 cccgcagcgg ccgaaaagaa gaaggtgatg ctcgttgacg atcacccgct ggtccgggag
     1861 ggacttggcc gattgatcaa tgatcaggcc gatttgatcg tcagcggcga ggcggacggg
     1921 ccggtgcgcg cgctggagct gctgaagcat tcccggccgg atctggtcgc gctcgatctg
     1981 tcgctcgccg gcggcgatgg cttggagctg tgcaagcagc tgcatgagtt atatccggag
     2041 ctgccggtcc tgattatctc gatgcatgat gagtcgctgt acgccgagcg ggcgctgcgc
     2101 gccggggcgt cgggttacgt catgaagcag gcaccgcagg agcaggtgat gcaggccatc
     2161 cgccggacga tgggcggtga aacgtggctg agcgataaga tgtcggccaa gctcctgcgc
     2221 tcgatccgcg gggcgcgcgc cggcgcggat gtgtcaccgc tggaacggct gacggatcgc
     2281 gagctcgaaa tcttccgcat gatcggacag gggctaagcg tgaaggcgat cgccgatgcg
     2341 ctgttcctca gccccaagac gatcgaggcg cacaaggagc acatcaagca gaagctgaac
     2401 ctggagacga gcaacgatct tcttcagtac gcgattcagg cgcgcgcaca gacggggtga
     2461 cgccggcgcc ggcgtgcgta ccggacaatt cacctgacgg gacaataggg cgaactcccg
     2521 atgcgcatct atctgataca cgtgtaatgt agatgcgatc gcgaggtgcg agtactgctt
     2581 cacccgccca tcgaagaatt ggaactgggt cggcgggccg tgcgggcgaa gatctgcccc
     2641 ggctgctatc aaagcgccag ttccgagcga gcgctcgatc aactcacccc gcgctcttgc
     2701 gaacccggtt gcacgatctt caccaatctg ccggcgatgc acctggccgt tgccaatcgg
     2761 ctgacggtgc tggccgacgc ggacaaggcg gccgagcaag tcgtctgcga aacgtgccac
     2821 gcccggccaa cctcggggga attctgcgcc gcgttctcct cgcgcacctg cccgctcagc
     2881 cggtatggcc gtgaagtcat cgacttgctg cagcgcatgc gcgaccgcca cgttcgcgtg
     2941 tcgtgattgt ttcccctcgc cttgtgcgcc gggacatctt cgacactcgt agtagaccct
     3001 ttgtgcgcgc ccgcgtcatc ctgaggctga ggatgacaat cgatagtgcc atgagcggat
     3061 gcagatgaaa cgactatccc cctaaaaccg cccctccaca atcctcgact ccgccgtcaa
     3121 ctcccccgac tgctccaccc gcagtcccgg cagccactgc tgtggcgttg cacgcccggt
     3181 catccacacg atccacacgt gcccgcacgc gcgcagccgg tccagcgtcg ggttgtccgc
     3241 gggcttggtc agcgtcgcga cgaccgccgg catcgccggt agcgcgtaat gctgaaacgc
     3301 cgtatatgac accgcgacca gcagcgggtc ggttgacgcc aggaccagcg catcgccggc
     3361 gcgcaggcgc ggggcgacga tctcgaccgg cgtgcgaaag tcatacttcc acggcggcac
     3421 atatgccgcc ggcaaccgca acaacgcgaa cgtcaccgcc agcgccggcg gtacgaaagc
     3481 ccatcgcccc cgcggcaccg cggcggcgat cagcacgcac gccgcggggg tcgcaacgag
     3541 cgtgtagcga agcacgcgca gctgcagcgt gccgcgcgcg aggtccaggc acaggaccag
     3601 gccgaccgcg cagatgatcc acagcacgaa cagccgcagg ttcggccggc gaacgaaggc
     3661 gatcggcagc agcaaccaga gcaccccggc gacaagcgtg acgaaattcg accaaccgct
     3721 gggcgacggc tccgcgagga agcgcgccgg caggcggtcc aggtccagca gcacgcgggc
     3781 cgcatgaccg gggccggcgt ggtcttgcac ccagccgtag tacgcatcca cgttcggatg
     3841 ctgccgcaac atgaatgggc cccaggtggc cataaagacg acgccagcga tcgccatggc
     3901 ggcgatggcg agcatgcggg cacgcccgcc aagggcgagc agggcgtaca gcccgaaggc
     3961 gaacgcagcg cccacggcga agtaatgggt cagcgccatc gcgagcgtcg cggcgccgag
     4021 cgcgatggcg gcgccgatcg tggcgcgctg tcgcatgcgc gccagcgccc acgccgccgc
     4081 gagcgtgaac gtcatcagcg gcatgtaacg cccggcttcc tgcgagaact cgatctgcgg
     4141 ccccgtcacc gccagcagca ggcacgccca cagggcggct gaggtgccga cgaacggccg
     4201 cgcgacgaag tacagcagcg ccacggcggc aaccgagaag acgacgccga ccgctcgcac
     4261 atgcgcttcg ctgtcatcgc ggaacagcgc ttcgaaagcg cgcacaagca ggatgtacag
     4321 cggcgggtgg gcgtcttcac gcacgacggc gggcaggatg tccgcccgcg gccgggcgtg
     4381 gtcataccgc gtcgcgaccg gttgcgtggg cggtaccacc tggtccatcg gcgtgcgcag
     4441 gaacacgagc ccccagccgt tggcggtgga gagggtacag aattcgtcgg tccagtaacc
     4501 gagggcggag aggttgtaga tccgcaatgc ggcggcggcg atgaggatca gcgtgagcag
     4561 caatcccgcg agcggaatgc ggcgaggcgg aggcgccggc gccgcgttgg cggtcacaga
     4621 atcaaggcgc gcttcgctgc gggcgggcat gcgtgtggtt tagcggctcc tcgaccagcg
     4681 gccgcgttca cgcggcgtgt ctttccggtc ctctcgcccg gtactcccgg agagggctgc
     4741 ggttttgaat gacgaatgac gaagtccgaa tcccagaatg aaatgacgaa atccgaatga
     4801 cgaaacggcg cgatccgcgc gcgtgttcgt catccgtcat tcatcattcg tcattcgtca
     4861 ttcgtcattc gtcattcgtc attcggattt cattcgggga ttcggacttc gtcattcgga
     4921 tttcctcact cgaaatcacc ctcaccccag ctctctcccg gggtaccggg agagggggta
     4981 agacggttgt gccgcgcgag gggcgttgtg gctaaacggc gaactacaca ccggccggcg
     5041 ccggttccgg cgtcgccgcc gcggcgggcg ccttcgccgc ggggttgtcc gcggttttgc
     5101 cgtaggtgcg cgctcgcggt ttcactaggc tggtgtccac cggcgcgtag ctgatgaccg
     5161 ccgaatcact cgcggcgtcg taggtcgcga tcgtggcttt caggaagttc gcgtcgtcgc
     5221 gctcggcatg ttccggcttg taatgcgcgc cgcgcgattc gttgcgagcg agcgcgcctt
     5281 tcaagatcgc ctcggactcc aggatcatgt cgcgcgtcgc gcgggcgaat gagaggttct
     5341 ggttcgtcca catgccggtg tccgacagct tcacgcgctt gaagcggtcc ttccattcct
     5401 ggcatttttc cagcgtcaat tcgagcttct cgttgtagcg aacgaccgtg cagttctcgg
     5461 tcatccactt gcccatctcc tgccacagca ggtaagggtt ttcgttgccg tcgttggtga
     5521 cgagccaatt ctggcgatcc ttctcctgct tctcgaacct ttcaaacgtg gcggccggca
     5581 cgtcatcggc ggcggtcttg gcggtgtcgg tgatgtagtt cttgatgcac gtcccgccga
     5641 acaggccgtc gaaaatgcag ctgagcaggc tgttggcccc gaggcggttc gcgccgtgat
     5701 acgcgaagga gacttcgccc atcgcataca ggcccgggat gttggtgctc atgttgtcgg
     5761 ggtgaccgaa gaccaggccg ccggggctgg
//
LOCUS       DATIMH010000346        10232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5565249, whole genome shotgun sequence.
ACCESSION   DATIMH010000346 DATIMH010000000
VERSION     DATIMH010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10232
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5565249"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VLI90_05960"
     CDS             complement(<1..430)
                     /locus_tag="VLI90_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13783.1"
                     /translation="MKSRLLATDTLDVSGKILKNALSAIARHRGDFRPARSWPALLLI
                     MLAAVNTAEGPTLFGPPSIAFASAPASPTTQPTGIATADGPLKLIDLTFPSDPPINDP
                     QLTQIDPPKPEQIWRAVRSHLAPDPAIDSDPLSLIPATTPS"
     gene            645..1544
                     /locus_tag="VLI90_05965"
     CDS             645..1544
                     /locus_tag="VLI90_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13784.1"
                     /translation="MIEVACSSCGKQYRLADELSGKRVRCKECGETIAVPELAPAGAI
                     ATLNDDDIAVAPAPAPVAAPSPAKPAAPMFAAAPSPMPGAPAGKRWGFRINRFAIVLG
                     VIGVFLLMTGVKEMRLANAASPTPQDISCLELEKSGPGKNAYVRVSKFLTTGGGVYQK
                     EEHADNWQMVWLPLIAKDGEYAQRVRQMVLSGAIKTAADLPAPDHVRVIVKSSKVRTQ
                     ADIDSFDDQHKTLTGMVINQVETMSEKEKRLLQESYPGSDLDACWIIEEGREPASSAK
                     YLGMMGGGLALLLLGLWMTFKPA"
     gene            complement(1751..2470)
                     /locus_tag="VLI90_05970"
     CDS             complement(1751..2470)
                     /locus_tag="VLI90_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13785.1"
                     /translation="MSFAPTTGLCLDCNYSLRDLPTPRCPECGREFDPADKTTFNPGR
                     PVPTYAAWAVGPISLPVYFVAIAGCATTIWRARLPGMSFSWTSPAMVGWAALAILWIA
                     WPLVRRLVLRHHGWPPAVIRPMGRRHWIVPLVMLAMVVMTSRMLPRRLAFAASRGAMD
                     QLAAEVMANPQSKFDNRWVGLFQAKNVRAIPGGMKFTAEDDDIQFKAGFVYLPKVDPK
                     NVAWKAYRYIGNGWWSWREEG"
     gene            complement(2536..2916)
                     /locus_tag="VLI90_05975"
     CDS             complement(2536..2916)
                     /locus_tag="VLI90_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HSV13786.1"
                     /translation="MKIVSMTPEEICDRLLKLAVRLGKVVDALPDIRQGRHVAGQLIR
                     SGTSPMPNYEEGCAAENMDDFIHKLSICLKELRESRGWVKYIVEAQLLQKRKLAGVLD
                     ECEQMCAVIGKSLVTSKRNAGRMR"
     gene            complement(2954..3892)
                     /locus_tag="VLI90_05980"
     CDS             complement(2954..3892)
                     /locus_tag="VLI90_05980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV13787.1"
                     /translation="MKYRKLGNTGLKVSVVGVGTWQYGGEWGIDFTQPMVDAIFDKSR
                     QLGINLIDTAECYGDHTSEAFIGTATQRDREQWIIATKFGHKFHGPFNRTDERSAKDV
                     RVQVEASLKALRTDYVDLLQYHSVRDSEFFDIAVRDEIAKLMTEGKVRFIGNSIGNAA
                     IADDGTNHQAARSIEFNVSVLQVIYNRLDRRPDEGEHSVFRSAGEQDLGVLARVPLAS
                     GLLSGKYKPGASFPQGDIRAQRKREELDAKLKEVEQIRHNEVPPGVDMATWALAWCLQ
                     HPAVTAVIPGCKSAQQVEQNAKAAELAQADHPQAWR"
     gene            4106..5248
                     /locus_tag="VLI90_05985"
     CDS             4106..5248
                     /locus_tag="VLI90_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13788.1"
                     /translation="MRATSWLIVLGCCCVETIALGQDAAVPASAPATTQPGITLVNLH
                     LKQAVPEDVFFMLGKQGGIKFAPNGNVWDQEAMQVPNDVDFTNRPFWQAVHEVCGMWH
                     VTLQNDFNSGRRLMVNAPQMSPNGQTWHALPTSESDGFVVEAVGFNQSINYGNPAGNS
                     CGVQLRTYVDPSLRVQTFNGVAKVLEAADEAGHSMLLEHANGMFYGGGMQQRSFVFDC
                     SVPLKYPPENAGKKIAHLKCILQLRAGDKMDSMVVEDPLKSPQVNKEFGDLRVTFRSL
                     KKQGPSNYELKLTITHNDDDGRGGNSDWPMMQSAQLLDSMGRSFVNGGGGGGSSRNGV
                     YNYTVNYNAPGGEDAPSGDPAKWVIELPTNTHVMRVPVEFSDLPMP"
     gene            complement(5398..6258)
                     /locus_tag="VLI90_05990"
     CDS             complement(5398..6258)
                     /locus_tag="VLI90_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HSV13789.1"
                     /translation="MTQFLSIIALCLSFASNITSAAPTTRPTIFICGDSTAKNTGKDK
                     AGQPLQGWGTAIAAYFDPAKVAVENVAHAGKSSMTYYNGDWPSVLPRIHAGDYLLLVF
                     GINDGTTPDGLGDETREGRNGDLQHTYGWYMSKMATDAQAKGAHVFLLTVTTRNIWTN
                     PKVRYHDGTPATTLPADYDPKQDRIERGTGGGKYTQWTKEIGQRLHIPVFDLTNYCAD
                     RYEQMGREAVNRLYSDHNHTYPPGADLVAQSIVSGLKALKDSPFVDLLSEKGKAPPPA
                     SAKYVNDNAP"
     gene            complement(6420..8156)
                     /gene="murJ"
                     /locus_tag="VLI90_05995"
     CDS             complement(6420..8156)
                     /gene="murJ"
                     /locus_tag="VLI90_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HSV13790.1"
                     /translation="MSKAAIAPSMGTFTAPAGSPAAAPSKTKSFVQHAKLIGFLTLIS
                     RFFGLGREIVAGHYLGTALVGSAFTVAFVVPNLFRKLFGEGALSAAFIPLYSQALKRD
                     TEQEANDFAAAAVNLLCAILLGLTVIGEAIIFGLLLIDRDFRPDRVLTLKLAAIMLPY
                     VLLICGGAFLSGILQVHKRFGPPAAAPIILNLCHIFVLLIGAKLVGLGKNTPLDMIVA
                     KQTTLAYWLASFVLVAGVMQVAVLMPALRQVGFRFKFIPHFWTPAIKRMLMLSLPVAL
                     GAGVLQLSVLLDKGLSMVLMQGVDNAGHAVTHFHFFGHLVRFPMELGAPRRLDLAQFL
                     YQFPLGIFAIALATAIFPGLSADALDKDRDRFRGVLRHGIEASLWEGLPASVGLMLVA
                     GPTAKLLFQHGQIHAHDAELIARSTVIYAGAIWAFSLLQIINRAYYAVHDTVTPLVMS
                     IVNIVLNLAIEIPLLWTNLGESAMAVGTLVSFAVQAVIMLWMLDRKIGGLNLRQSAAT
                     VGKMILATLVMGIACYALQHSPLYPYSAGRMSWAMELAIMLIVGASIYFGMCALLGVS
                     LLRDLFPRRQRT"
     gene            complement(8195..8650)
                     /locus_tag="VLI90_06000"
     CDS             complement(8195..8650)
                     /locus_tag="VLI90_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSV13791.1"
                     /translation="MDDRLHIHRRRLPHWELKGSTYFLTFRVARGELTMPQRLIVLQH
                     IRSGDGKFYDLLAAVLMPDHGHAILTPRDNVELSRIMKGIKGVSAKLVNDSRGTRGQL
                     WQDESFDRIIRDQAELDEKLNYVITNPVRAELVVDPWAYEALYINPNAG"
     gene            complement(8829..9887)
                     /locus_tag="VLI90_06005"
     CDS             complement(8829..9887)
                     /locus_tag="VLI90_06005"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF002727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HSV13792.1"
                     /translation="MSQSMNPPPGPALRVLDANANRAREALRVLEDYARFVLNSDELS
                     AILKQHRHALADVTRPWLADAILFRNTFDDVGKANKTASELQRDGVEDVVIAAGKRIG
                     EALRAIEEFAKTFSPETAADVEDLRYRFYDTEQVLAGTLRAANRFASVRLYVLITESA
                     CRRPWLEVAMEAIKGGADCLQLREKGMEGGELLARAKQLVAICRPRGIISIINDRPDI
                     AILSGADGVHVGQGDLPATEARKIVGREPIVGVSTHSIEQAKQAVLDGADYIGVGPVF
                     ASATKPREILPGLACAREVAEKIKIPAVAIAGINERNVDEVIAAGIKRIAVTAAVTGC
                     EEVRDAAVRLKQKLLAAT"
     gene            10018..>10232
                     /locus_tag="VLI90_06010"
     CDS             10018..>10232
                     /locus_tag="VLI90_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13793.1"
                     /translation="MLAFAGFSIIVLISFVLTCLPVQLSTEGGRADERAVHIEPYAFC
                     IDDTALVQETVEEEADDAEEPSLMIST"
BASE COUNT         1871 a         3049 c         3363 g         1949 t
ORIGIN      
        1 tggatggcgt cgtcgcgggg atgagcgaga gcggatcgga gtcgatcgcc ggatcgggcg
       61 cgaggtgcga gcgcaccgcc cgccagattt gttccggttt tggcggatca atttgcgtca
      121 gctgcgggtc attgatcggc gggtctgacg gaaacgtcaa atcgatcagc ttcaatggcc
      181 catcggccgt tgcgattccg gttggctgag tcgtcggcga cgccggcgcg ctggcgaacg
      241 cgatgctcgg cgggccgaag agggttggac cctcggcggt gttgacggcg gcgagcatga
      301 tcagcagcag cgccggccag ctgcgcgcgg gccggaagtc accgcgatgg cgcgcgatgg
      361 cggatagggc gtttttgagg atcttgccgc tcacatcgag cgtatcggtc gcaaggaggc
      421 gcgatttcac cggcggcgca gaattggcgg gcagtgccgg ttgtaacggc ggggcgggtg
      481 aggattatcg gtctcactgt gttcgcgtag cggtagcctg cggggctccc gctacgcatt
      541 acggagtcag cagtccgtcc tgcgcatcga agacgctttt tgattgaccc cgaaatccgc
      601 gggcgatatc acgtgcggat caaccaactc gcggggatgc gcccatgatc gaagtcgcct
      661 gttcaagctg cggaaagcaa tatcgcctgg cggatgaact gtcgggcaaa cgcgtgcgct
      721 gcaaggaatg cggcgagacg attgccgtgc cggagcttgc gccggcgggc gccattgcga
      781 ctttgaatga tgatgacatc gccgtcgcgc ccgcgcccgc gccggttgcc gcgccgtcgc
      841 cagcgaaacc ggcggcgccg atgtttgccg ccgcgccatc gccgatgccg ggcgcgccgg
      901 ccggaaagcg atggggattc cggatcaatc gcttcgcgat cgtgctcggt gtcatcggcg
      961 tctttctatt gatgacgggc gtgaaagaga tgcgcttggc aaacgcggcg tcgccgacgc
     1021 cgcaggatat ttcctgcctc gagctggaga agagcggccc cggcaaaaac gcgtatgtgc
     1081 gcgtgagcaa atttctcacc accggcggcg gggtgtatca gaaggaagag cacgcggaca
     1141 actggcagat ggtctggctg ccgctgatcg ccaaggacgg cgaatatgcg cagcgcgtgc
     1201 ggcagatggt gctgagcggc gccatcaaga ccgcggccga cctgcccgcg ccggatcacg
     1261 tgcgcgtgat cgtcaagtca tcgaaggtcc gcacgcaggc ggacatcgac agcttcgacg
     1321 atcaacacaa aacgctgacc gggatggtca tcaaccaggt cgagacgatg tcggagaaag
     1381 aaaaacggct gctgcaggag tcgtaccccg gcagcgactt ggacgcgtgc tggatcatcg
     1441 aagaaggccg cgaaccggca tcgagcgcga agtatttggg aatgatgggc ggcggtttag
     1501 ccctgctgct gctgggcctg tggatgacgt tcaaaccggc gtaattaaat gcaaccgccg
     1561 agggcgctga gagcgccgag cgaaacgccg agaagaaaat acagaagcgt ttttaaccgc
     1621 agattacgca gattcagagg aaggttcttc caatctgcga aatctgcgta atctgcggtt
     1681 acaaactctt tctctgctcg gcgtctgcct cggcgtgctc ggcgccctcg gcggttgcat
     1741 tttcaagtct tcatccctct tcccgccatg accaccaacc gttgccgatg tagcggtagg
     1801 ctttccacgc gacgtttttt ggatccacct tgggcagata gacgaagccg gctttgaatt
     1861 ggatgtcgtc gtcttcggcg gtgaacttca tcccgccggg gatggcgcgg acgtttttcg
     1921 cctggaagag gccaacccag cggttgtcga atttgctctg tggattggcc atcacttcgg
     1981 cggcgagctg gtccatcgcg ccgcggctgg cggcgaaggc cagccggcgc ggcagcatac
     2041 gcgaggtcat cacgaccatc gccagcatga ccagcggcac gatccagtgt cgccggccca
     2101 tcggacggat caccgcgggc ggccaaccgt ggtgccgcag cacgagccgg cggacgagcg
     2161 gccaggcgat ccagaggatc gccagcgccg cccaaccaac catcgcgggc gaggtccagg
     2221 agaagctcat gccgggcaag cgggcgcgcc agattgtggt cgcgcagccg gcgatcgcga
     2281 cgaagtagac gggcaaggag atcgggccga cggcccaggc cgcgtaagtc ggaacgggcc
     2341 ggccagggtt gaacgtggtc ttgtcggctg gatcgaactc gcgcccgcac tcggggcaac
     2401 gcggggttgg gaggtcgcgc agcgaatagt tgcaatcgag gcagagtccg gtggtgggag
     2461 cgaacgacat agcagttgta cgtctcaccg ctatgacggt ttcaaacagc agcgctttcg
     2521 gcggtgcgaa tccggtcacc tcatgcgtcc ggcatttcgt ttcgatgtga cgagtgactt
     2581 cccgatcacc gcgcacattt gctcgcactc gtcgagcacg cccgcgagct ttcgcttctg
     2641 aagcagttgt gcctcaacaa tgtatttcac ccacccgcgc gattcgcgca gctccttcag
     2701 gcagatactt agcttgtgaa taaaatcatc catgttctcg gctgcgcacc cctcttcata
     2761 gttcggcatc ggcgaagtac ccgatcggat gagctgcccg gcgacgtgtc gtccctggcg
     2821 aatatcgggc agcgcatcga caaccttgcc aagacggacc gcaagcttta acaggcgatc
     2881 gcagatttct tcaggtgtca tagaaacaat tttcaattga caattgtcaa tttttaattg
     2941 tttctcctcc ctcctaccgc cacgcttgcg gatgatctgc ctgcgccagc tccgccgcct
     3001 tcgcgttttg ctcaacctgc tgcgcgctct tgcatcccgg gatcaccgcc gtcaccgcgg
     3061 ggtgttgcag gcaccacgcc agcgcccagg tcgccatgtc cacgccgggt ggaacttcgt
     3121 tgtggcgaat ctgctcgacc tccttcagct tcgcgtccag ctcctcgcgc ttgcgctggg
     3181 cccgtatatc accctgcgga aacgaggcgc cgggcttgta cttcccgctc agcagcccgc
     3241 tggctagcgg gacgcgcgcg agcacgccca ggtcctgctc gccggcgctg cggaagacgc
     3301 tgtgttcgcc ctcgtccggc cggcggtcca ggcggttgta gatcacctgc agcaccgaca
     3361 cgttgaactc gatcgatcgc gccgcctgat ggttcgtgcc atcgtcggcg atggcggcgt
     3421 tgccgatcga gttgccgatg aagcgcacct ttccttcggt catcagcttg gcgatctcat
     3481 cgcgcacggc gatgtcgaag aactcgctgt cgcgcacgct gtggtactgc aatagatcga
     3541 cgtagtcggt ccgcaacgct ttcagcgacg cttccacttg cacgcgaacg tccttggccg
     3601 accgctcgtc ggtgcggttg aacggcccgt ggaatttgtg gccgaacttg gtagcgatga
     3661 tccactgctc gcgatcgcgc tgcgtggccg tgccgatgaa cgcttcggac gtgtgatcgc
     3721 cgtagcactc ggcggtgtcg atcagattga tgccgagctg gcgcgacttg tcgaagatgg
     3781 cgtcgaccat cggctgcgtg aagtcgatgc cccactcgcc gccgtactgc caggtgccga
     3841 cgccgacgac ggagactttg agaccggtgt tgccgagctt gcggtatttc atgcgcggca
     3901 gtttaacgcg ggaagcggag gcggggtcta gaggcgattc acagccgcag gcgacagcca
     3961 gcggggggag cgcgagttgg cgctcgacct gctggctatc gcctgcggct gtgacggaag
     4021 tgccttcgtg acttcgtgca ttttctcgct cctcgggcaa caatcttcga gcatctgcgt
     4081 ctaaacgcac aacaaaagga aatgtatgcg ggcaacatct tggctcatcg tgctcggttg
     4141 ctgttgcgtc gaaacgatcg cgctggggca ggatgcggcg gtgcccgcct cggcgccggc
     4201 gaccacgcag ccggggatca cgctggtcaa cctgcacctc aagcaggcgg tgccggagga
     4261 cgtgttcttc atgcttggca aacagggcgg gatcaagttc gcgcccaacg gcaacgtgtg
     4321 ggaccaggaa gcgatgcagg tgcccaacga tgtggacttc accaatcggc cgttctggca
     4381 ggcggtgcac gaggtctgcg gcatgtggca cgtcacattg cagaacgatt tcaacagcgg
     4441 ccggcggctg atggtgaatg cgccgcagat gtcgcccaac gggcagacgt ggcacgcgct
     4501 gccgacatcc gagtcggacg ggttcgtcgt cgaagcggtc ggcttcaacc agagcatcaa
     4561 ctacggcaac cccgcgggca attcatgcgg cgtgcagctg cggacgtacg tagacccatc
     4621 gctgcgcgtg cagacgttca acggcgtggc gaaggtgctc gaggccgcgg atgaagcggg
     4681 gcattcgatg ctgctcgaac acgctaacgg gatgttctat ggcggcggga tgcagcagcg
     4741 ttcgttcgtg ttcgactgca gcgtgccgct gaagtatcca ccggaaaacg cgggcaagaa
     4801 gatcgcgcat ctgaagtgca tcctgcagct gcgcgccggc gacaagatgg attcgatggt
     4861 ggttgaagat ccgctgaagt cgccgcaggt caacaaggag ttcggcgatc ttcgcgtgac
     4921 gtttcggtcg ctgaaaaaac aggggccgtc gaattacgag ttgaagctca cgatcacgca
     4981 caacgacgac gacggccgcg ggggcaacag cgattggccg atgatgcaga gcgcgcagct
     5041 attggattcg atgggccggt ccttcgttaa cggcggtggc ggtggtggga gcagccgcaa
     5101 cggcgtgtac aactacaccg tcaactacaa cgcccccggc ggcgaggatg cgcccagcgg
     5161 tgatccggcg aagtgggtga tcgagttgcc gacgaacacg cacgtgatgc gcgtgccggt
     5221 ggagttttcg gatttaccga tgccatgatg ggggtcgtca ttggagcgcg cgttacagcc
     5281 gcttgcggct tagcgtctgt tttgattgaa cgatgttaat cgcgttgtcg attgtgggag
     5341 tgtactgcgc gccgaacacc gggccctgcg ggccgccgct ataaggggac aggggggtca
     5401 cggcgcgttg tcgttgacgt acttcgcgga tgccggaggt ggggcttttc ctttttctga
     5461 caacaagtct acgaacgggc tgtccttcag cgccttcagg ccgctgacga tcgactgcgc
     5521 gacgaggtcg gcgcccggcg ggtaggtgtg attgtgatcg ctgtacaggc gattgaccgc
     5581 ctcgcggccc atttgttcgt agcggtcggc gcagtagttg gtgagatcga agacggggat
     5641 gtgcaggcgc tggccgattt ctttggtcca ctgcgtgtac ttcccgccgc cggtgccgcg
     5701 ttcgatcctg tcttgcttgg ggtcgtagtc ggccggcagc gtcgtggcgg gcgtcccgtc
     5761 gtggtagcgg accttcgggt tcgtccagat gtttcgcgtg gtgacggtca gcaggaaaac
     5821 gtgcgcgccc ttcgcctggg cgtcggtggc catcttcgac atgtaccagc cgtaggtgtg
     5881 ctggaggtct ccgttgcggc cttcgcgcgt ttcgtcgccg aggccgtcgg gcgtggtgcc
     5941 gtcgttgatg ccgaagacga gcaagaggta gtcaccggcg tggatccgcg gcaggacgct
     6001 gggccaatcg ccgttgtagt aggtcatgct cgacttgccc gcgtgagcga cgttttcgac
     6061 cgcgactttc gccggatcga aatacgcggc gatcgcggtg ccccagccct gcagcggctg
     6121 gcccgccttg tctttgccgg tgttctttgc tgtggaatcg ccgcagatga agatcgtggg
     6181 ccgggtggtg ggggcggcgc ttgtgatgtt cgaggcgaat gacaagcaga gcgcgatgat
     6241 cgaaaggaac tgggtcaacc cccacgccgg gttcggagcg tactcgcgac gacccggatt
     6301 cttctgggcg cgagagagaa agaatccggg tcgtcggagt atccttcgac ttcgctcagg
     6361 acaggcctcc gaacccggcg tgggggcttt tgaatgtgtc tcaagaaacg aacgactcat
     6421 cacgtcctct gccgccgcgg gaacaggtcg cgcagcaggc tgacgccgag cagcgcgcac
     6481 atgccgaagt atatgctcgc gcccacgatc agcatgatgg ccaattccat cgcccagctc
     6541 attcggcccg cgctatacgg gtagagcggc gagtgctgca gcgcgtagca cgcgatgccc
     6601 atgacgagcg tcgcgagaat catcttgccg accgtcgccg cgctctgccg caggttcagg
     6661 ccgccgatct tgcggtccag catccacagc atgatgaccg cctgcaccgc gaagctgacc
     6721 agcgtgccga cggccatcgc gctttcgcca aggttcgtcc acagcagcgg aatttcgatc
     6781 gccaggttca gcacgatatt cacgatcgac atgaccagcg gcgtgaccgt gtcgtgcacc
     6841 gcgtaatacg cgcggttgat gatctgcagc agcgagaacg cccagatggc gccggcgtag
     6901 atgacggtcg atcgcgcgat cagctcggca tcgtgcgcgt ggatctgccc gtgctgaaac
     6961 agcagcttgg ccgtcggccc ggcgacgagc atcaggccga cgctggcagg caggccttcc
     7021 cagaggctgg cttcgatgcc gtggcgaagc acgccgcgga agcggtcgcg gtctttgtcc
     7081 agcgcatcgg cgctgaggcc ggggaagatc gcggtagcca gcgcgatcgc gaaaatgccg
     7141 agcgggaatt ggtagaggaa ttgcgccaag tccaaccgtc gcggtgcgcc cagctccatc
     7201 gggaagcgca cgagatgccc aaagaagtgg aagtgcgtga cggcgtggcc ggcgttgtcc
     7261 acgccttgca tcagcaccat cgacagaccc ttgtcgagca acaccgacag ctgcagcacg
     7321 cccgcgccga gggcgacggg aagtgacaac atgagcatgc gcttgatcgc cggcgtccaa
     7381 aaatgcggga tgaacttaaa gcggaagccg acttgccgca gcgccggcat cagcaccgcg
     7441 acctgcatca cgccggcgac gagcacgaag gacgcgagcc agtaggcgag cgtcgtctgc
     7501 ttcgcgacga tcatgtccag cggcgtattc ttgcccaggc cgaccagctt cgcgccgatc
     7561 agcagcacga agatgtggca gaggttgagg atgatcggcg ccgcggccgg ggggccgaag
     7621 cgcttgtgga cttgcaggat gccgctgaga aacgccccgc cgcagatgag cagcacgtac
     7681 ggcagcatga tggcggccag cttcagcgtc agcacgcggt ccggccggaa atcgcggtcg
     7741 atcagcagca ggccaaagat gatcgcttcg ccgatcacgg tgaggccgag caggatcgcg
     7801 cagagcaagt tgaccgcggc ggcggcgaaa tcattcgctt cctgctcggt gtcgcgcttc
     7861 agtgcttgtg aatagagcgg aatgaacgcg gcggagaggg cgccttcgcc gaatagcttg
     7921 cggaagagat tgggaaccac gaacgcaacg gtgaacgccg agccgactaa ggcggtgcca
     7981 agataatgcc ccgcgacgat ttcgcggccg aggccgaaga agcggctgat gagcgtgagg
     8041 aacccgatca gcttcgcgtg ttggacgaag gattttgttt tcgatggcgc agcagccggc
     8101 gaacctgcgg gtgcggtgaa ggttcccatc gatggtgcga ttgcggcctt agacatcctt
     8161 gcctctggtg gggcagacat tcttgtctgc cgcgtcaacc cgcgttcggg ttgatataca
     8221 acgcttcata cgcccaggga tcgactacga gttcagccct gacgggattc gtgatgacgt
     8281 aattcagctt ttcatccagc tccgcctggt cgcgaatgat gcgatcgaac gactcgtctt
     8341 gccacagttg cccacgcgtg ccgcgcgagt cgttcaccag ctttgcggaa acgcctttga
     8401 tgcccttcat gatgcgcgac agttcaacgt tgtcgcgcgg tgtgaggatc gcgtgaccgt
     8461 ggtccggcat caagaccgca gcgagaagat catagaattt tccgtcgccg ctgcggatgt
     8521 gctgcagcac gatgaggcgc tggggcatcg tcagttcgcc gcgggccacg cggaaggtga
     8581 ggaagtaggt cgagccttta agttcccagt ggggaaggcg acggcgatga atgtgcaggc
     8641 ggtcgtccat gggttcagat tcttggcagc ggtggggcag acattgctgt ctgccacggt
     8701 ctcaaccatt ggtggggcag acattcttgt ctgccacggg cggcggtact ccgccgcacg
     8761 tatcttctaa gaagaaacgg acggatgagt acatgcgccc gtggcaggca agaatgcctg
     8821 ccccaccatt aggttgccgc tagcaatttc tgtttcaatc gcaccgcggc gtcgcggact
     8881 tcttcgcatc ctgtgactgc cgccgtcacc gcgatgcgct tgatccccgc ggcaattact
     8941 tcatcgacat tccgctcgtt gatgcccgca attgcgaccg cgggaatttt gatcttctcg
     9001 gcgacctcgc gcgcgcacgc caaccccggc aggatctcgc gcggcttggt cgcacttgcg
     9061 aagaccggcc cgacgccgat gtagtcggcg ccgtcgagca ccgcctgctt ggcctgttcg
     9121 atcgagtgcg tgctgacccc gacgattggc tcgcggccga cgatctttct cgcctccgtc
     9181 gccggcaggt cgccttgtcc gacatgtacg ccatcggcgc cgctgaggat cgcgatgtcc
     9241 gggcgatcgt tgatgatcga gatgatcccg cgcgggcggc aaatggcgac cagctgtttc
     9301 gcgcgcgcca gcaactcgcc gccttccatt cccttctcgc gcagttgaag gcaatcggcc
     9361 ccgcccttga tggcttccat cgccacctcc aaccacggcc ggcggcaggc ggattcggtg
     9421 atcaacacat acagccggac acttgcaaag cggtttgcgg cacgaagcgt gcccgccaaa
     9481 acctgttcgg tgtcataaaa gcgatagcgg agatcctcga catcggctgc cgtttcagga
     9541 ctgaaggtct tggcaaattc ttcaatcgcc cgcagcgctt cgccgatgcg cttgcctgcg
     9601 gcgatgacca catcctcaac cccatcgcgc tgcaactcgc tcgccgtctt gttggctttg
     9661 cccacgtcat cgaacgtgtt gcggaacaag atggcatccg ccagccatgg ccgcgtgacg
     9721 tccgcaagtg catgtcgatg ctgctttaag atcgccgaca gctcgtcgct attcagcaca
     9781 aagcgggcgt aatcttccag cacccgcagc gcttcgcggg cgcggttggc gttggcgtcc
     9841 agcacgcgaa gggccggacc gggcggggga ttcatgctct gtgacaccgg gggccgatta
     9901 taggaccagt tgccgccatc gccagacgag cggcgaatgc gctctggtcc cctcgctcgg
     9961 cgacctgcgg tcagcagtac cgggcgaggg ggaaagaagg gtcttttcat gctgttaatg
    10021 ctcgccttcg ccgggttttc catcatcgtg ctgatctcgt tcgtgctgac ttgtctgccg
    10081 gttcagctga gcaccgaagg cggccgcgcg gatgaacgcg cggtgcatat cgagccgtac
    10141 gccttctgca tcgacgacac ggcacttgtt caagaaacgg tcgaagaaga agccgacgac
    10201 gccgaagagc cgtcgttgat gatctccacc ca
//
LOCUS       DATIMH010000347         4023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1522930, whole genome shotgun sequence.
ACCESSION   DATIMH010000347 DATIMH010000000
VERSION     DATIMH010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522930"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VLI90_06015"
     CDS             <1..1373
                     /locus_tag="VLI90_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13794.1"
                     /translation="STGARARAKVLGHGLWRPCYKMSNSVSEALTFLRQLGTPDELST
                     RANSSYEPTTRPRVNAHIHLPPNFSAFDSVAQAIQLAGDQQIGVLGVSNYYDYDVYGD
                     FVAGARQRGIFPLFGLEIIALIDEYVQAKAKINDPGNPGKIYICGKGITRFAPFNAEA
                     QRLMNIIRGNDSKRMAEMVAKLSAVFAERGLPVNLSEADVVQMVVDRHGSKRERVYLQ
                     ERHACQAFQEAIFRKVPANQRVERLSNVLGLNGSPPKFGPGDAVKIQNEIRAYLMKAG
                     RPAYVPETFVSFDEAYRLILELGGIPCYPTLADGVTPITWFESPVEKLIAHLKQFGFH
                     CAEFIPIRNTPEVLSEYVKAMRAAGLVITAGTEHNTLDLLPIEPTCLNGVPVPDDVKE
                     IFWEGACVVAAHQFLTLNGKTGFVESSGAPNKEYADTETRIAAFKKLGAAVIQRYSER
                     ATKAHA"
     gene            1408..3444
                     /locus_tag="VLI90_06020"
     CDS             1408..3444
                     /locus_tag="VLI90_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSV13795.1"
                     /translation="MSPTTAWPEFSTSDAPIDALVKLSRFYGANPEFVLAGGGNTSVK
                     VGDRLFVKGSGHALATISADGFVELDRKKLESLLKSKLSDDRMKREEEFKTAIMASRL
                     AAERGQRPSVEALLHHLMPGTFVVHTHSTLVNMLTCSARGKALMRGVLGEDAIWIPEV
                     DPGFVLSQTLQQALAEYKTATGRDCPRAVLMQNHGLVIAGDTPEAIRKNTDWLISVLH
                     AHLEKTPATDPFGAVTRIEDPKRVRQLVTLIGPALRGLLASGETLPIVAFDDSHDVMN
                     LVGGVEAQTVATTGPLTPDQIVYCKSFPLWIDPPDSVEAIRSAIEQHRAKHKADPHVV
                     LVKGLGMFTIGDDLNGARTAQAVYTDAIKIMAGARRLGGVNYLNADLRRFIEEWEVES
                     YRKGIAAAGRAGGRAAGKVVIVTGAAQGFGLEISQDAASQGAYVVLTDINAEGTAKAA
                     AALNERLGKTRAIGLPINVTSGESIEEAIYQTVRVFGGVDCFISNAGVLKAGSVKTQS
                     EKDFDFVTNVNYKGFFLCTQKVAPILATQHVAKRDYWSDIIQINSKSGLQGSNRNGAY
                     AGGKFGGIGLVQSFALELVEDGVKVNAICPGNFFDGPLWSDPVNGLFAQYLKSGKVPG
                     AKTIEDVKRSYESKVPMNRGCTTADVVKAIYYLMEQKYETGQAVPVTGGQVMLK"
     gene            3511..3930
                     /locus_tag="VLI90_06025"
     CDS             3511..3930
                     /locus_tag="VLI90_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HSV13796.1"
                     /translation="MDVFDFHARNESRLEPELEDLAFAVIGAAIEVHRELGPGLSELH
                     YRKALSHELSLRNIPHACEVPIAIAYKGVDIGEGRLDIIVADRLILELKAVERLTEVH
                     RSQAITYLCVTKNKLALLINFNVAILKDGIKRVINSI"
BASE COUNT          841 a         1188 c         1288 g          706 t
ORIGIN      
        1 gtagcacggg cgccagagcc cgtgccaagg tgctcggaca cgggctctgg cgcccgtgct
       61 acaaaatgtc gaactctgtc tctgaagctt tgacgttcct tcgccagttg ggcacacccg
      121 acgagctctc gacgcgcgcc aacagtagtt acgagccaac aactcgcccg cgggtgaacg
      181 cccacattca cctgccgccc aacttttcgg cgttcgattc ggtcgcgcag gcgatccagc
      241 tcgccggtga tcagcagatc ggcgtgctcg gcgtcagcaa ctattacgac tacgacgtct
      301 acggcgactt cgtcgccggc gctcggcagc gcggcatctt cccgctcttt ggcctggaga
      361 tcatcgcgct catcgacgag tacgttcaag ccaaagcgaa aatcaacgac cccggcaacc
      421 ccgggaaaat ctacatctgc ggcaaaggca tcacgcggtt cgcgccgttc aatgccgagg
      481 cgcagcgatt gatgaacatc atccgcggga acgattcgaa gcgcatggcc gagatggtcg
      541 ccaagctgag tgccgtcttc gccgagcgcg gattgccggt gaacctgagc gaagcggacg
      601 tggttcagat ggtcgtcgat cgccacgggt cgaagcgcga gcgcgtatac ctgcaggaac
      661 gccatgcctg tcaggcgttt caggaagcga tctttcgcaa ggtgccggcc aaccagcgcg
      721 tcgagcggtt atcgaacgtg ctcggcttga acggctcgcc gcctaagttc gggcccggcg
      781 atgcggtgaa gatccagaac gagattcgcg cttatctcat gaaggccggc cggccggcgt
      841 atgtgccgga gacgttcgtc agcttcgacg aagcgtatcg attgatcctc gagctcggcg
      901 gcatcccgtg ttacccgacg ctggccgacg gcgtgacgcc gatcacctgg ttcgaatcgc
      961 cggtggagaa gctgatcgcg cacctgaaac agttcggctt ccactgcgcc gagttcatcc
     1021 cgattcgcaa cacgcccgag gtgttaagcg agtacgtgaa ggcgatgcgg gccgccggcc
     1081 tggtcatcac cgccggcacg gagcacaaca cgctggacct gctgccgatc gagccgacgt
     1141 gtttgaacgg cgttcccgtg ccggacgacg tgaaggaaat cttctgggaa ggcgcgtgcg
     1201 tcgtggcggc tcatcagttt ctgacgctga atgggaagac cggttttgtc gaaagcagcg
     1261 gcgcgccgaa caaggaatac gcagatacag aaacacgaat cgcggcgttc aagaagctcg
     1321 gcgctgctgt cattcaaaga tattcagagc gcgccactaa agcccacgca tagccatgcg
     1381 tgggctttcg gcagagcagg aacccttatg tcaccgacca ccgcttggcc tgagttctca
     1441 acgtccgatg cgccgattga cgcgctggtg aagctctcgc gcttctacgg cgcgaacccc
     1501 gagttcgtcc tcgccggcgg cgggaacact tcggtcaaag tcggggaccg cctgttcgtc
     1561 aaaggcagcg ggcatgcgct ggccaccatc agcgccgatg gcttcgtcga actggatcgc
     1621 aaaaagctcg agtcgctgct gaagagcaaa ctgagcgacg atcgcatgaa gcgcgaggaa
     1681 gaattcaaaa ccgccatcat ggcgtcgcgc ctggcggccg agcgcggtca gcgccccagc
     1741 gtcgaagcgc tgctgcacca cctgatgccg gggacattcg tcgtccacac gcacagcacg
     1801 ctggtgaaca tgctgacctg cagcgcccgc ggcaaagcgc tgatgcgcgg cgttctgggc
     1861 gaagacgcga tttggattcc cgaagtcgat cccggcttcg tcctgtcgca gacgctgcag
     1921 caggcgctgg cggaatacaa aaccgccacc ggccgcgatt gcccgcgggc tgtgctgatg
     1981 cagaaccacg ggcttgtgat tgccggcgac acgccggagg cgattcgcaa gaacacagat
     2041 tggctgattt cggtcctgca cgcgcatctg gagaaaacgc cggcgacgga tccgttcggc
     2101 gctgtgacgc gcatcgaaga cccgaagcgc gtgcgccagc tggtgacgct gattggacca
     2161 gcgctgcgcg gattgctcgc gagcggcgag acgctgccga tcgtggcgtt cgatgattcg
     2221 catgacgtga tgaacctcgt cggcggcgtc gaagctcaga ccgtcgcgac cacggggccg
     2281 ctcacgccgg atcagatcgt gtactgcaag tcgttcccgc tgtggatcga tccgccggat
     2341 agtgtcgagg cgattcgcag cgcgatcgaa cagcatcgag ccaagcacaa ggccgacccg
     2401 cacgtggtgc tggtgaaggg gctgggcatg ttcacgatcg gcgacgactt gaacggcgca
     2461 cgcaccgcgc aggccgtgta caccgatgcg atcaagatca tggccggcgc tcgccggctc
     2521 ggcggtgtga attacctgaa cgcggatttg cggaggttca tcgaagagtg ggaggtcgag
     2581 tcgtaccgca aaggcatcgc cgccgccggt cgcgcgggcg gtcgcgccgc ggggaaagtc
     2641 gtcattgtca ccggtgcggc gcaggggttc ggcctggaga tttcgcaaga cgcggcgtcg
     2701 cagggcgcgt atgtcgtgct caccgacatc aacgccgaag gcacggccaa ggccgcggcg
     2761 gcgctgaacg agcggctcgg caagacgcgc gcgatcggct tgccgatcaa cgtgaccagc
     2821 ggcgaatcga tcgaggaagc gatctatcaa acggtccgcg tcttcggcgg cgtggattgc
     2881 ttcatctcca acgccggcgt gctgaaagcc ggcagcgtga agacgcagtc ggaaaaagat
     2941 ttcgacttcg tcaccaacgt gaactacaag gggttcttcc tctgcacgca gaaggtcgcc
     3001 ccgatcctgg ccacgcagca cgtggccaaa agagattact ggtccgacat catccagatc
     3061 aattccaaga gcgggttgca gggatccaat cgaaacggcg cctacgccgg cggtaaattc
     3121 ggcggtatcg ggctggtgca gtcgttcgcg ctggaattgg tcgaagacgg cgtgaaggtc
     3181 aacgcgatct gccccggcaa tttcttcgac ggcccgctgt ggtcggaccc tgtgaacggc
     3241 ctgttcgcgc aatacctcaa gagcggcaag gtgcccggcg cgaagacgat cgaggacgtg
     3301 aagcgcagct acgagtcgaa ggtccccatg aaccgcggct gcacgacggc ggacgtcgtg
     3361 aaggcgatct attatctaat ggaacaaaag tacgaaaccg gccaagcggt gccggtgacc
     3421 ggtggacagg tgatgctcaa gtaggcgagt gaaagagaac gcgaccgcga agacgcgaag
     3481 gcgcgaagac agacgcgaag gaggatccgg atggacgtgt ttgattttca cgctcggaat
     3541 gagtcgcggc tggaaccgga gttggaggat ctggcattcg ctgtgatcgg cgcggcgatt
     3601 gaggttcatc gcgagctagg accgggcttg agcgagctgc attatcggaa ggcgttgtct
     3661 cacgaactgt cgctgaggaa catcccgcac gcatgtgagg tgccgatcgc gattgcttac
     3721 aagggcgtcg acatcggtga gggacgattg gacatcatag tcgcggaccg attgatcctg
     3781 gaattgaagg cggtcgagcg gctcactgag gtccaccgat cacaggcgat cacatactta
     3841 tgtgtgacca agaacaagct ggcgcttttg atcaacttca acgtcgcaat cttgaaggac
     3901 ggcatcaaac gagtcatcaa ctcaatctaa aagttccgct tcgcggctgt cttcgcgtct
     3961 tcgcggtcgc attgaattcg attaaggaac gcacaaatga ccaccaccct cccagaaaag
     4021 cag
//
LOCUS       DATIMH010000348         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2387611, whole genome shotgun sequence.
ACCESSION   DATIMH010000348 DATIMH010000000
VERSION     DATIMH010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2387611"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1752)
                     /locus_tag="VLI90_06030"
     CDS             complement(142..1752)
                     /locus_tag="VLI90_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSV13797.1"
                     /translation="MTKLRRTLGVIALAGASALAWEGGNALVDNVRFARAEQRVESTH
                     EQLQSVQELSTVFRNVGKVIEPSVVQIEVHKTIKGARRQFMPDDDFLRRFFRDHGVPG
                     GGDDQVQPNDPNGGGGNNDAAPDDNGPGGGGDLEQMGTGSGVIIEVDGSDAYIVTNNH
                     VAGGATEMVVTLSDGRTIKGGKTLGADPKSDLAVVKIHADHLIAAKWGDSDQLDRGDW
                     VLAFGSPFGYVGSMTHGIVSALNRHDVGILGNQGYENFIQVDAPINPGNSGGPLVNIH
                     GDVVGINTAIASRSGAFSGIGFAIPSNQAKVIVKMLKEKGKVTRGWLGVSIADVQRDP
                     GLAKSFGYDQDNGVIVQQVIQGTPSTGKLQPGDIITGIDGKNVENVQQLRNQVALISP
                     GTTAKLQVFRNGKQTDVPITIGEQPEDVMALGPKGSGIAPKAGNAESGEALGMRLTNV
                     NDDAIQKYGLSSDVKDGALVTAVEPRSLAAKAGLQPGDVITRVGGESVSDAKEASDAI
                     AKQDVNKGIRLYITNKLGSRFVFIQNEK"
     gene            complement(1790..>2258)
                     /locus_tag="VLI90_06035"
     CDS             complement(1790..>2258)
                     /locus_tag="VLI90_06035"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HSV13798.1"
                     /translation="DAGVAVMAHLGLRPQSVGLLGGYRFQGRTSEQADLIVSTAMQMQ
                     NAGAAALLIEAVPPEVSEAVVRATNIPVIGCGAGPACHGSVIVMHDALSLSPRRPRFV
                     PQLAELNRPMISAFADYVRRVSSGQYPAAEHQYEMPAEERSKFTSKRTAARPA"
BASE COUNT          358 a          760 c          672 g          468 t
ORIGIN      
        1 tcaccctcac cctaaccctc tcccatgagt acatgggcga ggggaccgaa tcggctgatg
       61 gtggcgcgac cgtcaaaaaa aaacgctgga tgaagatcat cttcatccag cgtttggttt
      121 tcgttcactc gctgacccgg atcacttctc gttctgaatg aacacgaacc gtgatccaag
      181 cttgttggtg atgtacagcc ggatgccctt gttcacatcc tgcttggcga tcgcgtcgct
      241 ggcttccttg gcgtcgctga ccgactcgcc gcccacgcgg gtgatgacgt cgccgggttg
      301 caggccggct ttggcggcga gcgaacgcgg ctcgaccgcc gtcaccagcg cgccgtcctt
      361 cacatcgctg ctcaggccgt acttctgaat ggcgtcgtcg ttcacgttcg tcagccgcat
      421 gcccagcgct tcaccgcttt cggcattgcc ggccttgggg gcgatgccgc tgcccttggg
      481 gcccagcgcc atcacgtctt ccggctgctc gccgatggtg atcggcacgt cggtttgctt
      541 gccgttgcgg aagacctgca gcttggcggt cgtgccgggc gagatcagcg cgacttgatt
      601 gcgcagctgc tgcacgttct cgacattctt gccgtcgatg ccggtgataa tgtcacccgg
      661 ttgcagcttg cccgtgctgg gcgtgccctg gatcacctgc tgcacgatca cgccgttgtc
      721 ctggtcatag ccgaagctct tggccaagcc cgggtcgcgc tgcacgtcag cgatgctgac
      781 gcccaaccac ccgcgggtga ccttgccctt ttccttcagc atcttcacga tgacctttgc
      841 ctggttggac ggaatggcaa agccgatccc ggagaacgcg ccgcttcggc tggcgatggc
      901 cgtgttgatg ccgaccacgt caccgtggat gttcaccagc ggaccaccgc tgttgccggg
      961 gttgatcggg gcgtcaacct ggatgaagtt ctcgtagccc tggttgccca ggatgccgac
     1021 gtcatggcga ttgagcgcgc tgacgatgcc gtgcgtcatc gatccgacgt aaccaaacgg
     1081 gctgccgaag gccagcaccc agtcgccgcg gtccagctga tcgctgtcgc cccatttggc
     1141 ggcgatcaga tgatcagcat ggatcttcac cacggccagg tcgctcttgg ggtcggcccc
     1201 cagcgtcttg ccgcccttga tcgtgcggcc gtcgctcagt gtgacgacca tctcggtcgc
     1261 tccaccggcg acgtgattgt tggtgacgat gtatgcgtcg gagccatcga cttcgatgat
     1321 cacaccgctg ccggtgccca tctgctcgag gtcgcccccg ccgccgggac cgttgtcgtc
     1381 tggcgcggcg tcattgttgc caccgccgcc gttcggatcg ttcggctgca cctgatcgtc
     1441 acccccgccg ggcacgccgt ggtcgcggaa gaaccggcga aggaaatcgt catccggcat
     1501 gaactgccgg cgggcgccct tgatcgtctt gtggacctcg atctgcacga ccgatggctc
     1561 gatcaccttt ccaacgttgc ggaagacggt ggataattct tgcacgctct gcaactgctc
     1621 gtgcgtcgat tccacccgtt gttccgcgcg ggcgaaccgc acgttgtcca ccaatgcgtt
     1681 accgccttcc cacgccagcg cggacgcgcc ggccaaggcg atcacgccca atgtccttct
     1741 caacttcgtc atcgttaagt ctcctcagtt tctgtacagg ccgacccgct taagcgggtc
     1801 gggccgctgt gcgcttgctt gtgaacttcg agcgttcctc cgcgggcatc tcatactgat
     1861 gctccgccgc cggatattgt ccgctcgaca cgcgccgaac gtaatcggca aaagcggaaa
     1921 tcattggtcg attcagctca gccagctgcg gcacgaaccg tggccggcgg ggtgacaggc
     1981 tcaacgcatc gtgcatcacg atcacgctgc cgtggcaggc ggggccggcg ccgcacccga
     2041 tcacggggat gttggtcgcc cgcacgacgg cctcgctgac ttccggcggg accgcctcga
     2101 tcagcagcgc ggccgcgccc gcgttctgca tttgcattgc agtagataca atcagatcag
     2161 cctgctctga cgtgcggccc tgaaaacggt acccaccgag cagacccacc gattgtggcc
     2221 gcaaacccag gtgtgccatc accgccacac ccgcgtcc
//
LOCUS       DATIMH010000349         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4120601, whole genome shotgun sequence.
ACCESSION   DATIMH010000349 DATIMH010000000
VERSION     DATIMH010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4120601"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VLI90_06040"
     CDS             complement(<1..763)
                     /locus_tag="VLI90_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSV13799.1"
                     /translation="MSELRGEPETTEIRTAGRSTGSGYYTAAPRGIKKPKSLQRPGGG
                     SRAYAGDGMLLHQDHSPTSSPSQTVAADSDSAGQWVNIRPGLRQLVTPRGPWFQFNDA
                     AGFEWNTYELSISHLPAALHGFRILQVADVHCWRHWQVAYDQLIERVRADEPDLMVFT
                     GDMVDDLWKPLPALPIARRLLNNLRAKHGMFGVRGNHDLKVRQTNFTGTALKLIDGQR
                     VVLETASGELELIGLPGPDREDLSDDFLRSIPPKQT"
     gene            762..1202
                     /locus_tag="VLI90_06045"
     CDS             762..1202
                     /locus_tag="VLI90_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13800.1"
                     /translation="MPSPRPQNRVFYALCANAAVLVLILCALISRGGGSQLGPSAAFA
                     QQQPPIAGGGGVFVMPAQLSTNVWGCFLMDIDNQTLCVYQYSPGEKMLRLSASRTFKF
                     DRGLNNFNTSPPPLEVRDLVQREREALRVTEPATPKSPEVQKEQ"
     gene            1313..2404
                     /locus_tag="VLI90_06050"
     CDS             1313..2404
                     /locus_tag="VLI90_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSV13801.1"
                     /translation="MAKMFYNVSEAKAALGKSEEEIKQYVREGRLREFRDGAQLMFKA
                     DQVDALKAQLGGGGGGGIEPVDLGPSDSGAAIGLVDSRGGSGTGIGLADTGGIGSGVG
                     SGIGSGVGSGIASSGSGLSGGISGSGLSGLGSAGSGMMNLKEDTALAADLGMSGSVGG
                     VPSPARSAGSGTGLSGSAAGRGGSGINVFGEEPERVDPMAQTAISSSGSIGDLNLEGV
                     GSGSGLLDLTRESDDTSLGAELLDEISPGGKRPVAPPDTGAMTAVGGSGVGLEAPRGG
                     VIRGTAGVTYVEAPDPMGFALGGLALGTAAVMIVGVLALTAALFGVHIDWMDKLADRG
                     FAIAAAIMAALPIIFFLFFFLAGKGTAKR"
     gene            2697..3623
                     /locus_tag="VLI90_06055"
     CDS             2697..3623
                     /locus_tag="VLI90_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSV13802.1"
                     /translation="MARMHRLLGLAAVGLALTGCVPQEKYAALKLDDDRLREQLGKAQ
                     TETQQARAESEAYKRQLDAYTNAGGSRDALTKNLMDQNANLQAQLDALNAKYADAIGK
                     ISTGGTALPAALSNDLSAFAAQNPDLVEFDAARGIVKFKSDVTFAVGDATLTSKAKEV
                     LTRFASILNGAGASNYELLVAGHTDSAPVVNPETIRKGHKDNWYLSSHRAITVSEELI
                     AGGVSSRRLGAVGYADQRPIASNASEGGKQQNRRVEVLILPTSARSSGPSVAETAPTR
                     TQRTTAAAPKKRPAPSFNKDTAATDNRPAFNK"
BASE COUNT          710 a         1247 c         1224 g          634 t
ORIGIN      
        1 cggtttgttt cggggggatc gagcgcaaaa agtcgtccga taaatcttcc cgatccggcc
       61 cgggcaggcc gatcaattcc aattcgccgc tggcggtttc caggacgacg cgttggccgt
      121 cgatcagctt taatgctgtg cccgtaaagt tcgtctgccg gactttcaga tcgtgattcc
      181 cgcgcacgcc gaacatgcca tgcttggcgc gaaggttgtt cagcaaccgc cgggcgatcg
      241 gcagcgctgg cagcggtttc cagaggtcgt ccaccatatc gccggtgaag accatcaagt
      301 ccggctcatc ggcgcgcacg cgctcgatca gctggtcata cgccacctgc cagtgtcgcc
      361 agcagtggac gtcggccact tgcaggatgc gaaacccatg caacgccgcc ggcagatgag
      421 agatggataa ctcgtaggta ttccactcga accccgcggc atcgttgaac tgaaaccacg
      481 gcccccgtgg tgtcaccaac tggcgaagac cggggcgaat gttgacccat tgcccggctg
      541 aatccgaatc ggccgcgacc gtttggctgg gggaggaagt tggcgaatga tcctgatgca
      601 gcagcatccc gtccccagcg taggcgcgcg acccgccgcc gggccgttgt aaactctttg
      661 gtttcttgat gccgcgcggg gcggccgtat aatatccact gccggtcgat cggccagcgg
      721 ttcgaatttc cgtcgtttca ggctcgccac gcagttcgct catgccatca ccccgtccac
      781 agaaccgggt cttttacgcg ttgtgcgcca acgccgccgt cttggtattg atcttgtgtg
      841 cgctgatttc gcgcggcggg ggcagtcaac tcggaccgtc cgccgcgttc gcccagcagc
      901 agccgcccat cgccggtggc ggcggcgtgt tcgtgatgcc ggcgcagctt tccaccaacg
      961 tctggggctg cttcctgatg gatatcgaca accagacgct gtgcgtctac caatattcgc
     1021 ccggcgaaaa gatgctcagg ctgtccgctt cgcggacatt caagttcgat cgcggactga
     1081 acaacttcaa caccagcccg ccgccgctgg aagtgcgaga cctggtgcag cgcgaacgcg
     1141 aagcgctgcg cgtgaccgaa ccggcgacgc caaagtcacc ggaagtgcag aaggaacagt
     1201 aaacgtgaat acgtgattac gtgaatacgt gacaagagct gcgagccttt cacgtatcgc
     1261 gtattcgctc atcacggtcg atgcagatcg acacacaacg aggatcaccc ccatggccaa
     1321 gatgttctac aacgtgagcg aggcgaaggc cgccttgggt aaaagcgaag aggagatcaa
     1381 gcagtacgtg cgcgaaggcc ggctgcgcga attccgcgac ggcgcgcagc tcatgttcaa
     1441 ggctgaccag gtcgatgcgc tcaaggccca gttgggcggc gggggcggcg gcgggatcga
     1501 accggtggac ctcgggccca gcgacagtgg cgcggccatc ggactggtag acagccgcgg
     1561 gggcagcggc acgggcatcg gcttggccga caccggcggc atcggcagcg gtgtgggcag
     1621 tggcatcggc agcggcgtcg ggagcggcat cgccagctcc ggcagcggat tgtccggcgg
     1681 catctccggc agcgggctca gtggcctggg cagcgctggc tcgggcatga tgaacctgaa
     1741 ggaagacacc gcgctggccg cggacctggg catgtcgggc agcgtcggtg gtgtgccgtc
     1801 gcccgcgcgc agcgccggca gcggcacagg cctgagcggc tcggccgccg ggcgcggtgg
     1861 gtccggcatc aacgtcttcg gcgaagagcc cgaacgcgtt gatccgatgg cgcaaaccgc
     1921 catctcctct tccggctcga tcggcgatct caatctcgaa ggcgtcggct ccggttcggg
     1981 tctgctcgac ctgactcgcg aaagcgatga cacgagcctc ggcgctgagc tgctcgatga
     2041 aatttccccc ggcggcaagc gccccgtggc cccgcccgac accggcgcga tgaccgccgt
     2101 cggcggcagc ggcgtcggtc tggaagcccc gcgcggcggt gtcattcgcg gcaccgcggg
     2161 tgtcacctac gtcgaagcgc cggatccaat gggcttcgcc ctcggcggcc tggccctggg
     2221 caccgcggcc gtcatgatcg tgggcgtgct cgccctcacg gcggcgctgt tcggcgtcca
     2281 catcgactgg atggacaagc tcgccgaccg cggtttcgcc atcgccgctg ccatcatggc
     2341 cgcgctgccg atcatcttct tcctgttctt cttcctggcc ggcaaaggta ccgccaagcg
     2401 ataagatgac gcatcgactc aacccatacg cggggttcgg aggtatgccg acgacccgga
     2461 ttctttgccg cgactgggca gaagaatccg ggtcgtcggc gtatctccga acccggcgtg
     2521 ggaaaatttt cttcgcgtat tgcttgcatt tcgtaactcg cccggcaaaa tatgcgcatt
     2581 cgagcgcgtc gggcattccg tccacgcgtt cgaattgaca ttcgaaaccg cgtcccgcga
     2641 cgggacacca acggccggtg gaaccggctt attaggattt tgcacggagg catcgtatgg
     2701 cacggatgca tcgtctgctc ggtctcgcgg ccgtcggtct ggcactcacc ggctgcgttc
     2761 cccaggaaaa atatgcggct ctgaaactgg acgacgaccg tctgcgcgag cagctcggta
     2821 aagcccagac ggaaacgcaa caggctcgcg ccgagtccga ggcgtacaag cgccagctcg
     2881 acgcctacac caacgccggc ggcagccgcg acgcgctgac caagaacttg atggatcaga
     2941 acgcgaacct gcaggcgcag ctggacgcgc tgaacgccaa atacgcggat gcgatcggca
     3001 agatcagcac gggtggcacg gcactgccgg ccgcgctcag caacgacctg agcgccttcg
     3061 ccgcccagaa cccggacctg gttgaattcg acgcggcccg cggcatcgtg aagttcaaga
     3121 gcgacgtgac gttcgccgtc ggcgacgcga cgctcacctc caaggccaag gaagtgctga
     3181 cccgcttcgc gtcgatcctg aacggcgcgg gcgcgagcaa ctacgagctg ctcgtcgccg
     3241 gtcacaccga cagcgcgccg gtcgtgaacc ccgagacgat ccgcaagggt cacaaggaca
     3301 actggtatct gtcgtcccac cgcgcgatca cggtcagcga agagctgatc gccggcggtg
     3361 tcagcagccg gcgcctgggc gcggttggtt acgccgatca gcggccgatc gccagcaatg
     3421 ccagcgaagg cggcaagcaa cagaaccgcc gcgtggaagt gctgatcctg ccaacgtcgg
     3481 cccgcagcag cggtccctcg gtggcggaga ccgctccgac ccgcacgcag cgcaccaccg
     3541 ccgccgcgcc gaagaagcgc ccggcgccgt cctttaacaa ggataccgcc gcgacggaca
     3601 atcgtccggc gttcaacaag taagcaagcc ctgagcgatc gctcaagcag cggtccctct
     3661 cggacgctga catacaagcc ccgcacatgc acaccgatgc atgtgcgggg ctttttcgtg
     3721 tgggcgaata taatcgcatc ctcgccgaca gccctgtagc tgcgacgcct gcgtcgcctc
     3781 cgcgcgaaga ggcaacgcag gcgtcgcagc tacag
//
LOCUS       DATIMH010000350         4759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5566559, whole genome shotgun sequence.
ACCESSION   DATIMH010000350 DATIMH010000000
VERSION     DATIMH010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5566559"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VLI90_06060"
     CDS             complement(<1..590)
                     /locus_tag="VLI90_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSV13803.1"
                     /translation="MTTDVSKPPIAGDDVDVLRAIVEGTASSTGDAFFRSLVQHLARA
                     IDVRYAFIAEFSPSDSRTRVHTISYWAIDHFADNVTFDLSGTPCEDVVRGNLCHYPSE
                     VYKRFPQDQALVKMGIESYLGVPLRDRDGRHLGHLAVFDQRPMPPEPRRLYLFRIFAD
                     RAAAELARIRYEQRLAESEQRFKDLFEEAPIAYVHE"
     gene            844..1092
                     /locus_tag="VLI90_06065"
     CDS             844..1092
                     /locus_tag="VLI90_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSV13804.1"
                     /translation="MTCRDVIGFLDRYCDGELAPLERMRFTLHLSLCSNCRRYLASYR
                     TTIRLSKNAFADSSQPAGDEVPPELARAIMASVQKDRK"
     gene            1195..2046
                     /locus_tag="VLI90_06070"
     CDS             1195..2046
                     /locus_tag="VLI90_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HSV13805.1"
                     /translation="MSDDATTPAAWRLYVIEACLLGTFMISACAFTALLEHPSSLALR
                     AIPSDFARRALIGLAMGATAIALIYSPWGRRSGAHMNPAFTVSFLWLRKISGRHAAGY
                     ITGQFAGGFLGLLICRMVWPMLIRHPSVNYVITTPGRHGATAAWAGEFSIAFVLMAVV
                     LLLNQFPRVAKRTGYVAGLLVAIYITFEAPLSGMSMNPARTLGSAIPAGVFTGLWIYF
                     TAPVLGMLAAVELHVLAGKPRHLLCGKLTHCHKIPCIIRCNCTTHAGINGASEDQPWP
                     AQQTTTT"
     gene            2016..3629
                     /locus_tag="VLI90_06075"
     CDS             2016..3629
                     /locus_tag="VLI90_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HSV13806.1"
                     /translation="MASTTNDYDVIIIGSGAGGGTLAFKLAPSGKRILLIERGDYVPR
                     ETDNWSTLAVNVKAKYNTTEVWHDRDGKPLHPHTNYYVGGNTKFYGAALFRLRREDFS
                     EIRHFGGGISPAWPLSYDEFEPYYSQAERLYCVHGTRGEDPTEPTASAPYPFPSVSHE
                     PRIQQLHDDLARLGHKPFHVPLGIQLNESNRHTSPCIRCATCDGHPCLIGAKADAQVL
                     CVDRALEYPNFTLLTNAYVSRLETDDSGTEVKTVHVERGGAKERYSAQVVVVSAGAIN
                     SAALLLRSASDKHPRGLANGSDVVGRHYMGHVNSIMLAISRTPNPSVFQKTLALNDFY
                     FGDPSGDFQFPMGHVSFVGKLDAITLGAGAPAIAPGFTLELMAKHSLDFWLTSEDLPD
                     PDNRVTLDRDGKIVLSYTPNNEEGHRRLIKTLKQMLNHLDCHEHLIPRNLFVGQRIPL
                     AGVAHQNGTIRFGTDPKTSALNVNCRAHEVQNLYVIDASFFPSCGAVNPALTIIANAL
                     RVGDHLLRQLGVASVTSQSRQPTAQAVVA"
     gene            complement(3877..>4759)
                     /locus_tag="VLI90_06080"
     CDS             complement(3877..>4759)
                     /locus_tag="VLI90_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HSV13807.1"
                     /translation="AVAYDEAARELFGEHARLNFPDGVDPAALREAMETENYEAPFPP
                     PGMMGRHEACRVFEVSITTWVVWERKGRVQCGQWVPVPGGKPGRCKVYPIDEINRLLE
                     EYQRIGKPYADPDRAGCCRVPLRSLIHQYEAIISAEDLPLVEGRNWNWAPRSNSESGR
                     VTLATMTGPQTPLCRIITGANGYGARVMHLNGDPLDCRRENLIVRTCAEQTYTNRKRS
                     TTNGEPCSSRYKGVSWDRKREKWVAQIVKDKQHHHLGRFGDELAAAEAYDEAARELFG
                     EHARLNFPEGVDVALAA"
BASE COUNT          869 a         1623 c         1399 g          868 t
ORIGIN      
        1 tcctcatgca cgtaggcgat cggcgcttct tcgaacaaat ccttgaagcg ctgctccgac
       61 tcggccaggc gctgctcata tcgaatgcgc gccaactccg ccgcggctcg gtcggcgaaa
      121 atgcgaaaca ggtacaaccg ccggggttct ggtggcatcg ggcgctggtc gaacacggcc
      181 aagtgcccca ggtgccgccc atcgcgatcg cgcagcggca cgccgaggta gctctcgatg
      241 cccatcttga ccagggcctg atcctgcgga aaccgtttgt agacctccga gggataatga
      301 cagaggttgc cgcggacgac gtcctcacac ggtgtgccgg acagatcgaa ggtgacattg
      361 tcggcgaagt ggtcaatcgc ccagtacgag atcgtgtgga cgcgcgtcct gctatcgctc
      421 ggcgagaatt ccgcgatgaa cgcgtagcga acgtcgatcg cgcgcgccag gtgctggacc
      481 agagaccgga agaacgcatc gccggtgctg ctggcggtgc cttcgacgat ggcgcgaaga
      541 acgtcaacat cgtcgccggc aatcgggggt ttggaaacgt cggtcgtcac ggtcgctaag
      601 cgggctgcgg catcagtgga gcgctgcggc ggaggtggga cgtacaaccc accggaatgg
      661 ccttgccagt cgaacgatcc ccagcccgtg cgagcataat aacctctggc gttggggcga
      721 cgccagcggt tggggccgag ccatcgcgcg caaggagcgc ccggcgatta gatgcacact
      781 gaatatgttg cgttacaggc tggttgggtg tgacccgagc cgactcctcg gcatcaaacg
      841 agcatgacct gccgcgacgt catcgggttt ttggatcgat actgcgacgg ggagctggcg
      901 ccgctggagc gcatgcgctt cacgctccac ctgtcgctgt gctccaactg ccggcgctat
      961 ctagcgtcgt accggaccac gattcggctg agcaagaatg cctttgccga ctcgagccag
     1021 ccagcgggcg acgaggtccc gccggaactc gcccgcgcga tcatggcatc agttcaaaaa
     1081 gaccggaagt aacggcgggt tcccgccgag cgggcggtgt ctacgcggcc atcgcttgct
     1141 gttgcgtaaa tggttaggat tgtttgctcc gcacgcgggc gggtacgact ccacatgagc
     1201 gatgacgcca caacgccggc ggcctggagg ctctacgtca tcgaggcgtg cctgctcggc
     1261 acctttatga tttccgcctg tgccttcacc gcactgctgg aacatcccag ttcactggca
     1321 cttcgggcca ttccgtctga cttcgcgcgt cgcgcgctga tcggtttggc gatgggcgcg
     1381 actgccatcg cgctgatcta cagcccatgg ggccggcgat cgggcgctca catgaacccc
     1441 gcgtttaccg tcagttttct gtggctcaga aagatcagcg ggcgccacgc cgcgggttac
     1501 atcaccggcc aattcgctgg cggctttctc ggcctgctga tctgtcgcat ggtttggccg
     1561 atgctgatcc gccacccttc cgtcaactac gtcattacga cccccggccg ccacggggcg
     1621 accgcggcgt gggcaggcga attttcgatc gcatttgtcc tgatggccgt cgtgctgctg
     1681 ctgaaccaat tcccgcgtgt tgccaagcgc accggctacg tcgcgggatt gctcgtcgcg
     1741 atctacatca cgtttgaagc gccgctctcg ggcatgagca tgaaccccgc gcgcacgctc
     1801 ggctcggcca tccccgccgg cgtcttcacc gggctttgga tctatttcac cgcgccggtg
     1861 ttggggatgc tcgcggcggt ggaactgcac gtgctggcag gcaagccgcg acatctgctc
     1921 tgcgggaagc tcacacactg ccacaaaatc ccctgtatca tccggtgtaa ctgcacgacg
     1981 cacgcgggca tcaatggggc aagcgaggat cagccatggc cagcacaaca aacgactacg
     2041 acgtgatcat catcggcagc ggcgctggcg gtggaacact ggcgtttaaa ttggcgccgt
     2101 ccggcaagcg catcctgctg atcgagcgcg gcgactacgt cccgcgggag accgacaact
     2161 ggagcacgct cgccgtcaac gtcaaagcta aatacaacac caccgaggtg tggcacgacc
     2221 gcgatggaaa gccgctgcac ccgcacacga actactatgt cggtgggaac accaagtttt
     2281 acggggcagc gctgtttcgc ctccgccgcg aagacttcag cgagatccgg catttcggcg
     2341 gtggcatctc gcccgcctgg ccgctctcgt acgatgagtt tgagccctac tactcgcaag
     2401 ccgagcggct ctattgcgtg cacggcacgc gcggcgaaga tcccaccgag ccaaccgcgt
     2461 cggcgcctta tccgttccct tcggtcagcc acgagccgcg catccagcag ctgcacgatg
     2521 acctggcgcg gcttggacac aagccgtttc acgtgccgct cggcatccag ctgaatgaat
     2581 caaaccgcca caccagcccg tgtatccgct gcgcgacctg cgacggtcac ccatgcttga
     2641 tcggcgccaa agcagacgcg caggtgctgt gtgtcgatcg agccctggag tatcccaact
     2701 tcacgctgtt gaccaacgct tacgtctcac gtttggagac cgacgactcc ggcacggaag
     2761 tgaagacggt tcacgttgaa cgcggcggcg cgaaggaacg ttactcagca caggtcgttg
     2821 tcgtctccgc cggcgcgatc aactccgccg ccctgttgct tcgctcggcc agcgacaagc
     2881 acccgcgcgg cctggccaac ggcagcgacg tcgtcggccg gcattacatg ggccacgtca
     2941 actcgatcat gctggcgatc tccaggacgc cgaacccttc ggtgtttcaa aagacgctgg
     3001 cgctgaatga tttctacttc ggtgacccat ccggcgattt ccagttcccc atgggacacg
     3061 tctcgttcgt gggcaagctc gacgccatca cgctgggcgc gggtgcgccc gcgatcgcgc
     3121 cgggtttcac gctggagctg atggccaagc attcgctcga tttctggctc accagcgaag
     3181 atctgccgga tccggacaac cgcgtgacgc tcgaccgcga cggcaagatc gtcctgagct
     3241 acacgcccaa caacgaagag ggccatcgcc ggctgatcaa gacgctcaag cagatgctga
     3301 atcacctgga ctgccacgag cacctgatcc cgcggaacct gttcgtcggc cagcggattc
     3361 cgctcgcagg ggtagcccat caaaacggaa cgatccgatt cggcaccgat cccaaaacgt
     3421 cagcgctcaa cgtcaactgc cgcgcgcatg aggtccagaa cctgtacgtg atcgacgcga
     3481 gcttcttccc ctcctgcggc gcggtcaacc ccgccctcac gatcatcgca aacgcgctgc
     3541 gcgtgggcga tcacctgctg cggcaactgg gcgtcgcttc ggtcaccagc caaagccggc
     3601 agccgaccgc gcaggcggtg gtggcatgac cgcctccagg tccttcgacc cgatcggccg
     3661 cttggccttg gccagacacg cgtagatata aaaccgctcc ccgatgatcg tcgtcgcccg
     3721 cgaccgcagc tccggagcct gccctgagcg aagccgaagg gtcttgatcc gccgaccgca
     3781 ggtccccgca agatcagctc cgcgggagcc tgccctgagc ctgtcgaagg ggctggcgaa
     3841 tggacaacgc tcgcatatcc gttcacccgt cctggattac gctgccagcg ccacgtccac
     3901 accctcgggg aaattcaatc gcgcatgctc gccgaacaac tctcgcgccg cctcgtcata
     3961 cgcctcggcc gcggcgagct cgtcgccgaa gcggcccaga tgatggtgct gcttgtcctt
     4021 gacgatctgc gcgacccatt tctccctttt ccgatcccac gacacaccct tgtaccgcga
     4081 actgcatggc tcaccgttcg ttgtcgaccg cttgcgattc gtatacgtct gctccgcgca
     4141 agtccgcacg atcagattct cacgccggca gtccagcgga tcgccgttca ggtgcatcac
     4201 gcgcgcccca tagccgtttg ccccggtgat gatccggcac agcggcgtct gcggcccagt
     4261 catggttgcc aacgtcaccc gtccgctctc actattactg cgcggcgccc aattccaatt
     4321 ccgcccctca accagcggca aatcttccgc gctgatgatc gcctcgtact gatgaatcaa
     4381 gcttctcaac ggcacgcggc agcagcccgc gcgatccggg tccgcgtacg gcttgccgat
     4441 ccgctgatac tcctccagca gccgattgat ctcgtcgatc ggataaacct tgcaacgccc
     4501 cggtttcccc cccggcaccg gcacccactg gccgcattgc actcggcctt tccgttccca
     4561 aaccacccac gtcgttatcg agacttcgaa cacccggcac gcttcatgcc ggcccatcat
     4621 gcccggcggc gggaacggcg cttcgtaatt ctccgtctcc atcgcctcgc gcaacgccgc
     4681 cggatcaacg ccgtcgggga aattcaaccg cgcatgctcg ccgaacaact cgcgtgccgc
     4741 ctcgtcatac gcgaccgcg
//
LOCUS       DATIMH010000351         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3544847, whole genome shotgun sequence.
ACCESSION   DATIMH010000351 DATIMH010000000
VERSION     DATIMH010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3544847"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VLI90_06085"
     CDS             complement(<1..300)
                     /locus_tag="VLI90_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13808.1"
                     /translation="MGIDPVAARDRQTDSNIMETLAKRPLAEFPTGLAMARIQAPNYR
                     SQTTESYGHGAYSVIITRDIEKDEQLKRLEKLPMVNGVAPISRLLLPEDLSSDREL"
     gene            710..1774
                     /locus_tag="VLI90_06090"
     CDS             710..1774
                     /locus_tag="VLI90_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HSV13809.1"
                     /translation="MSRPLNEQLLSITRLGRNFTSTLDGTPDDAAAVVNPLYDGADPW
                     VARHGDTYYACNSTRDGHIEVWRSDSPLTRDHRATVWTPPVRGWNSRQVWAPELHHIA
                     GRWYIYYSASDGRNANHRMGVLRATSDDPQGSYEDLGQLYTGDDIAGKTNNRWAIDGT
                     VLQLRGQLYFIWSGWQDERDIQHLYIAEMSDAATISSSRVQICANNCHVWEHVGEDRG
                     ERGLHEGPAILRRHGRVFLIYSASGSWQHTYKLGMLWMDSDADPMDPRSWTKHEQPVF
                     ESTDDVFGVGHCSFTTTPDGQDVMLYHAKKSRRDGWDRRVHAQLFDWDEDGLPAFGQP
                     ITPGQPIRPPTRTTPQRRAA"
     gene            complement(1940..>3240)
                     /locus_tag="VLI90_06095"
     CDS             complement(1940..>3240)
                     /locus_tag="VLI90_06095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV13810.1"
                     /translation="QATQASRLRWLIIALVFFATIINFLDRLTISVLAVPICQSLHLN
                     NLQFASISTWFLLAYTISQGISGRVFDRIGTKLGFTISVVIWSTAAMAHSVARGLASL
                     SIFRALLGLGEAGNWPGATKVIAEWFPVRERALAMGIFNSGPSLGSVIAPPLIVWLNL
                     RYGWQAAFLVTGSLGFLWLFAWLLIYRPAPVAVEEAPPSSSEMPPQPLAASRKILEYE
                     SPLAIDDDAAWTWRELLLHRQVWAIICCRVLVDPVWWLYILWLPKYLGAARGLSLQQA
                     GWRTSIPFIAADIGALTGGFASGYLIQRRWSVDASRKTIVILATVPMMAGFFAPHASS
                     LVMALAYISCVTFGFQAWINNVQTLPTDLFPERAVATVAGLGGVGAGIGSMIFTLSTG
                     WVVDHFSYTPVLMTAGLLPAAGTAVLLALLGRVEPVHRRA"
BASE COUNT          635 a         1011 c         1095 g          499 t
ORIGIN      
        1 cagctcgcgg tcgctgctca gatcttccgg caacaaaagc cggctgatcg gcgcgacgcc
       61 gttcaccatc ggtagctttt ccaggcgctt cagctgctca tctttctcga tgtcgcgcgt
      121 gatgatgacg ctgtacgcgc cgtggccata gctctcggtg gtctgcgatc gatagttcgg
      181 cgcctggatg cgcgccatcg ccaagccggt tggaaattcc gccagcggtc gcttggccag
      241 cgtctccata atgttggaat cggtctgccg gtcgcgcgcc gcgacggggt cgatgcccat
      301 ggagcggaag tccgcggcgc cgcgcgggac cgtataagac gtggcacatc cttcaagcca
      361 tgcgacgcag gataatgcaa ggacgccgag cgtgagactg cggttcatgc catacctccc
      421 ggaaatggtt gccgggatcc taccaccgca agggctgggg gtggttcaga aaccgccggg
      481 cgtttgtaac cccgctgccg gcaagttgcc ggttgattcg gcgtaaacct tgtcgatccc
      541 gaacaccggg cggtaaccgg tgcttgggaa tcaaacatcg aaacttaggc cgcgaatctg
      601 ccgataaaag gtcggagcgt gcggcagtaa gcaccggcgg agccggattg tcgcaccgga
      661 ctgaaccgta aaaaccacgg tggccgttgg aacaacggca gagggaagaa tgtcgcggcc
      721 gctgaacgaa caactgcttt cgattacccg cttggggcgc aacttcacca gtaccttgga
      781 cggtaccccg gacgacgcgg cggcggtggt caacccgctg tatgacggcg ccgatccctg
      841 ggtcgcccgc cacggcgaca cttattacgc ctgcaactcg acgcgcgatg gccacattga
      901 agtctggcga tcggacagcc cgctgacgcg cgatcatcgc gcgacggtct ggaccccgcc
      961 cgtgcgcggg tggaacagcc ggcaggtgtg ggccccggaa ctgcaccaca tcgccgggcg
     1021 gtggtacatc tattacagcg ccagcgacgg gcggaacgcg aaccatcgca tgggcgtgct
     1081 tcgcgcgacc agcgatgacc cgcaggggtc gtacgaagac ctcggccagc tgtacaccgg
     1141 cgacgacatc gccggcaaaa cgaacaaccg ctgggcgatc gacggcaccg tgcttcagct
     1201 gcgcgggcag ctctacttca tctggtccgg ctggcaggac gagcgcgaca tccagcacct
     1261 gtatatcgcc gagatgagcg acgccgcgac catctcgtcg agccgggtgc agatctgcgc
     1321 gaacaactgc cacgtgtggg aacacgtcgg cgaagaccgc ggcgagcgcg gcctgcacga
     1381 aggccccgcg atcctccgcc ggcacggccg ggtgtttttg atctactccg ccagcggttc
     1441 ctggcagcac acgtacaagc tgggcatgct gtggatggac agcgacgccg acccgatgga
     1501 cccacgaagc tggacgaagc acgagcagcc ggtgttcgag tcaacggacg acgtcttcgg
     1561 cgtcggtcac tgctcgttca cgacgacacc ggacgggcag gacgtgatgc tgtatcacgc
     1621 gaagaagagc cgccgggacg ggtgggatcg ccgggtgcat gcgcagttgt tcgactggga
     1681 cgaagacggc ttacccgcgt tcggccagcc gatcacaccg gggcaaccga tccgcccgcc
     1741 gacgcgcacg acgccgcagc gccgtgcggc gtgatgattg cgtgagcgag agggcgctca
     1801 tgcttcacgt acgctaaaga aatcaaattt aaaagcgcag acaggcgggc acggcccatg
     1861 cgtctgcgca tgctcttcca gcctgtcaac gagtccgaac aaccaacgcc gccgcctggt
     1921 ggggaatcca cctgtcgtct caggcgcgcc gatgcacagg ctcgacgcgg ccgagcagcg
     1981 ccagcagcac cgcggtgccg gcggcgggga ggaggccggc ggtcatcagg accggcgtgt
     2041 acgagaagtg atcgacgacc cagccggtgc tgagggtgaa gatcatgctg ccgatgcccg
     2101 cgccgacgcc gcccaggccg gcgacggtgg cgacggcgcg ctcggggaac aggtcggttg
     2161 gcagggtttg gacgttgttg atccacgcct ggaacccgaa cgtgacgcag ctgatatacg
     2221 ccagcgccat caccagcgaa cttgcgtgcg gagcgaagaa gccggccatc atcggcaccg
     2281 tggcgaggat gacgatcgtt ttgcgggaag cgtcgacgct ccagcgccgc tggatcaggt
     2341 agccggacgc gaatccgccg gtgagggcgc cgatgtcggc ggcgatgaag gggatgctgg
     2401 tgcgccagcc ggcttgttgc aggcttaagc cgcgggcggc gccgaggtat ttggggagcc
     2461 agaggatgta gagccaccag acgggatcga cgagcacgcg gcagcagatg atcgcccaga
     2521 cctggcggtg taagaggagt tcgcgccagg tccacgccgc gtcatcgtcg atggcgagtg
     2581 gcgattcgta ctccaggatt ttccgcgaag cggcaagcgg ctgcggtggc atttccgatg
     2641 atgacggtgg cgcttcttca acagcaaccg gcgcgggtcg atagatcagc aaccaagcga
     2701 ataaccatag aaaccccaac gaccccgtca ccagaaacgc agcctgccag ccgtagcgca
     2761 ggttcagcca gacgatcagc ggcggggcga tgaccgatcc gagggagggg ccgctgttga
     2821 agatccccat cgccagcgcg cgctcgcgca cgggaaacca ctcggcgatc accttcgtcg
     2881 cacccggcca gttgccggct tcgcccaggc ccagcagcgc gcggaagatc gacaggctgg
     2941 ccaatccgcg cgcgacgctg tgcgccatcg cggcggtgga ccagatgacg accgaaatgg
     3001 taaagcccag cttggtcccg atgcggtcga acacgcggcc gctgataccc tggctgatcg
     3061 tgtacgccag cagaaaccac gtgctgatgc tggcgaactg caggttgttc aggtggaggc
     3121 tctggcagat cggcaccgcc agcacggaga tcgtcaggcg gtcgaggaag ttgatgatcg
     3181 tggcgaagaa gacgagggcg atgatcagcc accgtagccg cgacgcctgc gtcgcctgcg
//
LOCUS       DATIMH010000352         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2677783, whole genome shotgun sequence.
ACCESSION   DATIMH010000352 DATIMH010000000
VERSION     DATIMH010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677783"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1271)
                     /locus_tag="VLI90_06100"
     CDS             complement(123..1271)
                     /locus_tag="VLI90_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13811.1"
                     /translation="MTHLLHARTSLLATFVVAFATFLAPAFVHAQAAGGGTDATITED
                     DVIAGTMNIDFKTRTNSDTSGDLKEGSAALGAQDKYSLNLNVAKTTEFAGDIMRQPKL
                     SSSVLGRTKQNAKLAYNVNIAVLNPRDLKQKKNVGKWVGEIAIDPQTGAYDLARGESP
                     LRIAIDAVGRASSFTDKFGGKLQGKAEKKESLSSYTYNRIVGNKTVSVTVKKSDPMRF
                     DNIELAKGPAEVYPHTFVTGRLDYDYETGNWFTDGIHFKYNMDGRDVEDVVTGSIKWV
                     EDPDRATNGKGHYEFNLRFNEDKNKPAQSESAAFEKMSSEDAFFAVDNSIPCLTGKIE
                     YVDTFISGGTTPSSSKITYHLSANKLTKQQIENFFKLWMVCVGPTNDE"
     gene            complement(1494..>2024)
                     /locus_tag="VLI90_06105"
     CDS             complement(1494..>2024)
                     /locus_tag="VLI90_06105"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HSV13812.1"
                     /translation="HARQMLIARRLLERGVRFVQVWHGAGQPWDNHDDLEVNHRRLAQ
                     ESDQPIGALLKDLKQRGMLEDTLVIWGGEFGRTPTVEMPTPGSNAGKINGRDHNHYGF
                     TMWMAGGGVKGGHVHGATDEFGFKAVQDPVHVHDLHATMLQLLGFDHEKFTYRHAGRD
                     FRLTDVHGTVVRDLIA"
BASE COUNT          301 a          624 c          675 g          424 t
ORIGIN      
        1 ctactgggag agggttaggg tgagggggtg ccgatttgga cttgcgattt tggattgaga
       61 ggcgcgccat catcgcgtcg cctcttcaat ccaaaatccg caatccgaaa tccaaaatgc
      121 actcactcgt cgttcgtcgg cccgacgcac accatccaca gcttgaagaa gttctcgatc
      181 tgctgcttgg tcagcttgtt ggccgacagg tggtaggtga tcttgctgct gctcggcgtc
      241 gtgccgccgg agatgaacgt gtcgacgtac tcgatcttgc cggtcaggca cgggatggag
      301 ttgtcgacgg cgaagaacgc gtcctcgctg ctcatcttct cgaacgcggc cgattccgac
      361 tgcgcgggct tgttcttgtc ctcgttgaag cggaggttga actcgtagtg ccccttgccg
      421 ttggtggcgc ggtcggggtc ctcgacccac ttgatcgatc ccgtcaccac gtcctccacg
      481 tcgcggccgt ccatgttgta cttgaagtgg atgccgtcgg tgaaccagtt gccggtctcg
      541 tagtcgtagt ccaagcggcc ggtgacgaac gtgtgcgggt agacctcggc cgggcccttg
      601 gccagctcga tgttgtcgaa ccgcatgggg tccgacttct tcaccgtgac cgacaccgtc
      661 ttgttgccga cgatgcggtt gtaggtgtag ctgctgagcg attccttctt ctcggccttg
      721 ccctggagct tgccgccgaa cttatcggtg aaggagctgg cgcggccgac ggcgtcgatg
      781 gcgatgcgca gcgggctttc cccgcgggcg aggtcgtacg cgccggtctg cgggtcgatg
      841 gcgatctcgc cgacccactt gccgacgttc ttcttctgct tcaggtcgcg cgggttgagc
      901 accgcgatgt tgacgttgta cgcgagcttg gcgttctgct tcgtgcgacc gagcacgctg
      961 ctggacagct tgggctgccg catgatgtcg ccggcgaact cggtggtctt ggcgacgttg
     1021 aggttcaggc tgtacttgtc ctgcgccccg agggcggcgg agccttcctt caggtcgccg
     1081 ctggtgtcgg agttggtgcg cgtcttgaag tcgatgttca tcgtgccggc gatgacgtcg
     1141 tcttcggtga tggttgcgtc cgtgccgccg ccggcggctt gggcgtgaac gaacgccggc
     1201 gcgagaaacg tcgcgaaggc gacaacgaag gtcgcgagca gcgaagtgcg cgcgtgaagc
     1261 aagtgagtca tttcgatctc ctcccgagtt gcgatgaagt gccctgtgct gccgccgaac
     1321 cggtggcccg gccgcccatt tgccgaccgg gccgactcgc gcggcagtgc gcaaaatcat
     1381 aactcggggc gggagtgtcg caatcgcgca ccgcgtgcgc aattagaaat gctgccgctg
     1441 ttgataatgc gggcgggcgg tggcggttgt gcaacgccga gccgcgccga ggctcacgca
     1501 atcaggtcgc ggacgaccgt gccgtgcacg tcggtgaggc ggaaatcccg cccggcgtgg
     1561 cggtaggtga acttctcgtg gtcgaagccg agcagttgca gcatcgtcgc atgcaaatcg
     1621 tgcacgtgga cggggtcctg caccgccttg aacccgaact cgtcggtcgc gccgtgcacg
     1681 tgcccgccct tcacgccgcc gcccgccatc cacatcgtga agccgtaatg gttgtgatcg
     1741 cgaccgttga tcttgcccgc gttggagccc ggcgtgggca tctcgacggt tggcgtccgc
     1801 ccgaactcgc cgccccagat gaccagcgtg tcctctagca tgccgcgctg cttcaaatcc
     1861 ttgagaagcg cgccaatcgg ctgatccgat tcctgcgcca gccggcggtg gttcacttcg
     1921 aggtcgtcgt ggttgtccca cggctgtccg gcgccgtgcc acacctgcac gaagcgcacg
     1981 ccgcgctcga gcagccgccg cgcgatgagc atctgccgcg cgtg
//
LOCUS       DATIMH010000353         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_369290, whole genome shotgun sequence.
ACCESSION   DATIMH010000353 DATIMH010000000
VERSION     DATIMH010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369290"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..439)
                     /locus_tag="VLI90_06110"
     CDS             complement(32..439)
                     /locus_tag="VLI90_06110"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSV13813.1"
                     /translation="MLDWFKNLIKRSERPPQRDALGDRGENVAARYLRNQGYKIIVRN
                     FRCELGEIDIIARDGKTLVFVEVKTRAYDDPTPEDQVNTAKQHQLTKAAKIYLSRYGL
                     PQPPARFDVVAVVWPNGREPIIRHTPHAFEPTF"
     gene            complement(523..1248)
                     /locus_tag="VLI90_06115"
     CDS             complement(523..1248)
                     /locus_tag="VLI90_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13814.1"
                     /translation="MPIPRSCVIAFLCVCTVGLQLVHAEPAATQPFKPTSEYVQREIR
                     GWTVLVNGDLLSRDPPLSAACLDLLDAKLLDVERAVPPRALAKLKPVHIWLELHDPRF
                     PGGAYHPSRQWLIENGFNPDKAKSVELGNARNFLSWSKEQPMMVLHELAHAYHDQVLG
                     YDDPDIAAAYEAAKHDGLYEHVLRSNGHIERAYALNNDQEYFAELSEAFFGTNDFFPF
                     VRAEIGQHDPRMLKVLKDKWNRD"
     gene            1406..3316
                     /locus_tag="VLI90_06120"
     CDS             1406..3316
                     /locus_tag="VLI90_06120"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV13815.1"
                     /translation="MSVQLGLLRMPTMVGEPGQMPFRPRICLWVDQSSGMVLHFQLQE
                     QPGDPIRFTLESIVELANRIHGLPRQIQTREPNLAMALRPALADLGVEVIVRESLPML
                     DEAFESLGNFKDLGVKPEPSVLQSPGMTLDRLIAFAEAAKLFYEARPWWQLVDDDLIE
                     IESPAGPKGTRFAQMLGAGGREFGLGFVATRKVHEDMQAGLGVPRGGLWSLLFDELDY
                     LPFDDGQAWERHNLPVAHARAYPNFKKFMTSRPQGVLPTADELEWAEGLLRALAATSE
                     DELDSGRWTKDVQTASGPRTYQLSLPILLEEMAGGSAPNPNAVITSGRMAMERMMRGI
                     AQQVAEQGATSEADINRILKGVTAGAQLPEFKPTTPTEQAMELAYQAHDARGRRKVQL
                     ARQALAIDPDCCEALLILAGRNTDPEFALPRLRRATEAGERQLGPEVFAEDAGHFWMI
                     TETRPYMRARGTLAATLMRIEQYDEAIVHFQELLRLNPGDNQGNRYMLAQCLLYTNRL
                     DELDELLNRAEYKDEFSAEWAYTRTLLSYRRGGDSPEARQQLLEAHRTNPNVVSLLTG
                     KAKMRPGPPMPYSPGDMNEAVNCVMQLGAAWDETDGAIEWLQTVTAPLEKRTQPSHGE
                     GERQKKDKKKRR"
     gene            complement(3350..>3626)
                     /locus_tag="VLI90_06125"
     CDS             complement(3350..>3626)
                     /locus_tag="VLI90_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13816.1"
                     /translation="SQFIAKLDHFTEEELVHLNRLIVERLRIMRDIGAHQAMTQFRIG
                     QRVSFTDPRGREVGGVLTRYNRKSVSVIADDGTQWTVSPALLHADER"
BASE COUNT          706 a         1111 c         1174 g          635 t
ORIGIN      
        1 ccactttctc tgcctaccgc ctactgccta ctcagaacgt gggctcaaac gcgtgcggcg
       61 tgtgccggat gatgggctcg cggccgttcg gccagacgac cgcgaccacg tcgaaccggg
      121 ccggcggttg gggaaggccg tagcggctca ggtagatctt ggcggccttg gtgagttggt
      181 gctgcttggc cgtgttgacc tggtcctccg gcgtcggatc gtcgtacgcg cgggtcttca
      241 cctcgacgaa caccagcgtt ttcccgtcgc gggcgatgat gtcgatctcg cctagttcgc
      301 agcggaaatt gcggacgatg atcttgtacc cctggttgcg caggtaacgg gcggccacgt
      361 tttcaccgcg gtcgcccagc gcatcgcgct gcggcggccg ctcgctgcgc ttgatcaagt
      421 ttttgaacca gtccaacatg acccgcccag cgtaccgcga ccggcggcgg atgaatagtg
      481 cgcgagcttg cgatgtcttc cgcagcacgg gctccatgtc cgtcaatcgc ggttccactt
      541 gtccttcagc accttcagca tccgagggtc atgctggccg atctcggctc tcacgaacgg
      601 gaagaaatcg ttcgtgccga agaacgcctc gctcagttcc gcgaaatatt cctggtcgtt
      661 gttcagcgca tacgcacgtt cgatgtggcc attgctgcgc agcacgtgtt cgtaaagccc
      721 atcgtgtttc gccgcttcat acgcggcggc gatgtcaggg tcgtcgtatc ccaacacctg
      781 atcgtgatac gcgtgggcca gttcgtgcag caccatcatg ggctgctcct tgctccagct
      841 gaggaagttc cgcgcgttgc ccagttccac gctcttggct ttgtccggat tgaagccgtt
      901 ttcaatgagc cactgccgcg acggatgata cgccccaccg ggaaagcgcg ggtcgtgcag
      961 ctccagccag atgtgcaccg gcttcaactt cgccagcgcc cgcggcggca cggcgcgctc
     1021 gacgtcgagc agcttcgcgt cgagcaaatc caaacacgcc gccgacaacg gcgggtcgcg
     1081 actcagcaaa tcgccgttca ccaggacggt ccacccgcga atctcacgct gcacatactc
     1141 cgatgtgggc ttgaacggct gagtcgcggc cggttccgca tgcaccaact gaaggccaac
     1201 cgtgcacaca cagaggaacg cgatcacaca gcttcgcggg attggcatgc gcgccatcat
     1261 ccacgaacgg gtcgcaacaa ccaaagcgcg cgcccgagct gccggggaac aaatgacacg
     1321 tgcggcggaa gattttacga aattggaaag caccgctatt ctcacgccga tgtcagacga
     1381 atctcgccga ccgccgctat tgaacttgag cgtccaactg ggcctgctgc gcatgccaac
     1441 catggtgggc gagccagggc aaatgccgtt tcgcccgcgc atctgccttt gggtcgatca
     1501 atccagcgga atggtcttgc atttccagct gcaggaacag cccggcgatc cgattcgttt
     1561 cacgcttgaa tcgatagtgg aattggccaa tcgtattcac ggactgccgc ggcagattca
     1621 gacgcgtgaa ccgaatctgg caatggcgtt gcggccggcg ctggcggatc tgggggtcga
     1681 ggtgatcgtg cgcgaatcat tgccgatgct ggacgaggca ttcgaaagct tagggaactt
     1741 caaagacctg ggcgtgaagc cggaaccaag cgtgctgcag tcgccgggga tgacgctgga
     1801 ccgattgatc gccttcgccg aggcggccaa gctgttctac gaagcgcggc catggtggca
     1861 actggtggat gatgacctga ttgaaatcga atcaccggcg gggcccaagg gcacgcgctt
     1921 cgcgcagatg ctgggcgcgg gcgggcgcga gtttggtctt ggcttcgtcg ccacgcgtaa
     1981 agtccacgaa gacatgcaag cgggcctggg tgtgccgcgc ggcgggttgt ggagcctgct
     2041 ctttgacgag ctggattatc ttccgttcga cgacggccaa gcgtgggaac gccacaactt
     2101 gccggtggct catgccaggg catatccaaa cttcaagaag ttcatgacgt cgcgcccgca
     2161 gggcgtgctg ccgacggccg acgaactgga atgggccgag ggcctgctgc gcgcgctggc
     2221 ggcgacgagc gaagacgagt tggacagcgg acggtggacg aaggacgtgc agacggcatc
     2281 ggggccaagg acatatcaac tgtcgctgcc aattctgctc gaggagatgg caggcggcag
     2341 cgcgcccaat ccgaacgcgg ttatcacgtc gggccgaatg gcgatggagc ggatgatgcg
     2401 aggcatcgcg cagcaagtcg ccgagcaggg cgcgacgagc gaggcggata tcaatcgcat
     2461 cttgaaaggg gtgacggccg gcgctcaact gccggagttc aaaccgacaa cgccaaccga
     2521 gcaggccatg gaattggcat atcaagccca cgacgcccgt ggccggcgca aagtacagct
     2581 ggcacggcaa gcgctggcga tcgatccgga ttgctgcgag gcgctgctga tcctggccgg
     2641 gcgaaacacc gatccggagt tcgcgcttcc gcggcttcgc cgcgcgaccg aagccggtga
     2701 gcggcaactg gggccggaag tcttcgcgga agacgccggg catttctgga tgatcaccga
     2761 aacgcgccca tacatgcgcg cccgcggcac cctggcggca acgctgatgc gcatcgagca
     2821 atacgacgag gcgatcgttc actttcagga gctgctgcgc ctgaaccccg gcgacaacca
     2881 gggcaatcgc tatatgctgg cgcaatgcct gctttacacg aatcggttgg acgagctgga
     2941 cgagctgctt aaccgggcgg agtataagga tgaattctcg gccgagtggg cgtacacgcg
     3001 cacgctgctg agctatcgcc gcggcggcga ctcgccggag gcgcgccaac agttgctcga
     3061 agcccatcgg accaacccga acgtggtgtc actgctaact ggcaaagcga agatgcgacc
     3121 gggcccgccg atgccataca gccccggcga catgaacgag gcagtcaact gcgtgatgca
     3181 actcggggct gcctgggacg aaaccgacgg cgcgatcgaa tggttgcaga ccgtcacagc
     3241 cccgctggag aagcgcacgc agccatcgca cggtgaaggc gagcggcaga agaaggacaa
     3301 gaaaaagcgg cggtgacagc gaacgggcca acgcctgctg ccccggcggt caccgttcat
     3361 ccgcgtgcag caacgcgggg ctgacggtcc attgcgtgcc gtcgtcagca atgacgctga
     3421 cgctcttgcg gttgtaccgc gtgagcacgc cgcccacctc ccggccgcgc gggtccgtga
     3481 acgacacgcg ctgcccgatg cggaactgcg tcatcgcctg gtgagcgccg atgtcgcgca
     3541 tgatccgcag ccgctcgacg atcaggcggt tgagatggac cagctcttct tctgtgaaat
     3601 gatcgagctt ggcgatgaac tgggag
//
LOCUS       DATIMH010000354         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3545875, whole genome shotgun sequence.
ACCESSION   DATIMH010000354 DATIMH010000000
VERSION     DATIMH010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3545875"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..939)
                     /gene="hemF"
                     /locus_tag="VLI90_06130"
     CDS             complement(28..939)
                     /gene="hemF"
                     /locus_tag="VLI90_06130"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HSV13817.1"
                     /translation="MNETVLDERKAQARAWFESLQDKIIAAFESLEDDCHGPFNDEAP
                     ITPGRFERKPWSRDNHDGQPGGGGRMGILRGRFFEKAGVHTSTVFGTFAPEFAKQIPG
                     AAEDPRFWASGISLIAHPWNPNAPTVHMNTRFVVTSKAWFGGGADLTPVLMKRRTQED
                     ADTIAFHAAFEGACGRHAVADYPKYKAWCDEYFWLKHRNEPRGVGGIFYDYLNSANDA
                     ENSAWEADFAFTRDVGESFVKIYPEIARRNMTLPWNPLQRDEQLIRRGRYVEFNLLYD
                     RGTIFGLKTGGNVDSILSSMPPLVKWP"
     gene            complement(936..1436)
                     /locus_tag="VLI90_06135"
     CDS             complement(936..1436)
                     /locus_tag="VLI90_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HSV13818.1"
                     /translation="MTMISDDDPAARARRLTLALYQPDIPQNAGTMLRACACLGVGAA
                     IIEPAGFPISDRHFRRSGMDYLDHVAIDRHMNFASFQDWRRANRRRLVLLTTTGAVDY
                     TDFVFQPGDVLMVGRESAGVPADVHEAADARIVIPIRKDLRSLNVAVAAAMAMGEALR
                     QLGDRK"
     gene            1774..2292
                     /gene="petA"
                     /locus_tag="VLI90_06140"
     CDS             1774..2292
                     /gene="petA"
                     /locus_tag="VLI90_06140"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HSV13819.1"
                     /translation="MAEATTVEPTRRDFLYIATGAAAAVGVGAVAWPLISQMNPDAST
                     LALASTEVDMGSLPEGGIMTVKWRGKPVFVRHRTAKEIQEATSVPMAQLPDPEADSAR
                     VKKPEWLIVIGVCTHLGCVPLGHQGEYDGWFCPCHGSVYDTSGRIRKGPAPRNLDVPQ
                     YQFVADTKVRIG"
     gene            2371..>2567
                     /locus_tag="VLI90_06145"
     CDS             2371..>2567
                     /locus_tag="VLI90_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13820.1"
                     /translation="MSGPSTYVPKSAIGRWFESRLPVMGLIHSSFVAYPTPKNLNYFW
                     TFGGILSLMLGVQIITGVVLA"
BASE COUNT          455 a          858 c          807 g          447 t
ORIGIN      
        1 caacaagctc tggacgttca gcgccaccta gggccacttc accagcggcg gcatcgacga
       61 caggatcgaa tccacattgc cgccggtctt cagcccgaag atcgtgccgc gatcgtacag
      121 caggttgaat tcgacatagc ggccgcggcg gatgagttgc tcgtcgcgct gcaaggggtt
      181 ccagggcagc gtcatgttgc gccgcgcgat ctccggatag atcttcacga agctctcgcc
      241 gacatcccgc gtgaacgcga aatccgcctc ccacgcgctg ttttccgcat cattggccga
      301 attcagataa tcgtaaaaga tgccgccgac gccgcgtggt tcgttgcgat gcttcaacca
      361 gaaatattcg tcgcaccacg ccttgtactt cggataatcc gcgaccgcgt ggcgaccgca
      421 cgcgccctcg aaggcggcgt gaaacgcaat cgtatcggcg tcttcctgcg tgcggcgctt
      481 catcagcacc ggcgtcagat cggcgccgcc gccgaaccac gctttcgacg tcacgacaaa
      541 gcgcgtgttc atgtgcacgg tcggcgcatt cggattccac ggatgcgcga tcagcgaaat
      601 gcccgaagcc cagaagcgcg gatcttccgc cgcgcccggg atctgcttgg cgaattccgg
      661 cgcgaaggtt ccgaacaccg tcgacgtatg cacgccggcc ttctcgaaga agcgtccgcg
      721 caggatgccc atgcggccgc cgccgccggg ctgcccgtca tgattgtcgc gcgaccacgg
      781 cttgcgctcg aagcgccccg gcgtgatcgg cgcttcgtcg ttgaagggac cgtggcaatc
      841 gtcctcgagc gattcgaacg cggcgatgat cttgtcctgc aagctttcga accaggcgcg
      901 cgcctgcgcc ttgcgctcgt ccaacaccgt ctcgttcatt tcctgtcccc caactggcgc
      961 aacgcttcac ccattgccat ggcggccgcg acggcgacat tgagcgagcg caaatccttc
     1021 cggatcggaa tgacgatcct ggcgtccgcc gcttcatgga catccgccgg cacgcccgcc
     1081 gattcccgcc cgaccatcaa aacatcgccc ggctgaaaga cgaaatccgt gtaatccacc
     1141 gcgcccgtcg tggtcagcag cacgagccgc cggcggttgg cgcggcgcca gtcctgaaag
     1201 ctcgcgaaat tcatgtgccg gtcgatcgcg acgtgatcga ggtaatccat gcccgaccgg
     1261 cggaagtgcc tgtccgatat cggaaatcca gcgggctcga tgatcgccgc gccgacgccc
     1321 agacacgcgc aggcgcgcag catcgtcccg gcgttttgcg gaatgtcggg ctggtagagc
     1381 gcgagagtga gacgccgcgc ccgcgcggcc ggatcgtcat cggaaatcat cgtcatgctg
     1441 tgagcggtgc gtccttgtgc gacgggcgcc ggaaccgggg cggtcccgcg gacggacatc
     1501 gtgctttgcc cgatacgatg tcacgacagc gctactatgg acaagcgcgg cgaacgatgc
     1561 caaacaacgc ccgcccagca gcaggttccc gcgaccgggc cgtcgatgca cggccacggt
     1621 ggcgatgggt cgcattgcgg cgatagggcg ccagccgtaa ttcgaccggg tgaccggcgg
     1681 cggccggccc cgggcgcccg ccgcctggtt gtcgcgcgtg ctgcgcgcgg cgccgatgcg
     1741 ggcccgagac tgagaaggat ttgggggtta atcgtggcag aagcaacgac ggtcgagccg
     1801 acccgtaggg actttcttta tatcgccacc ggcgcagccg cggcggttgg cgttggcgcc
     1861 gtggcctggc cgctcatcag ccagatgaat ccggacgcgt ccacgctggc gctcgcctcc
     1921 acggaagtcg acatgggttc gctgcccgaa ggcggcatca tgacggtgaa atggcgcggc
     1981 aagccggtct tcgtgcgtca tcgcaccgcg aaggaaatcc aggaagccac gtcggtgccg
     2041 atggcgcaac tgccggatcc ggaagccgac tcggcccgcg tgaagaagcc ggaatggctg
     2101 atcgtgatcg gcgtctgtac gcatctcggc tgcgtgccgc tcggccatca gggcgaatac
     2161 gacggctggt tctgtccctg ccacggctcg gtctacgaca cgtcgggccg catccgtaag
     2221 ggcccggccc cgcgcaatct cgatgtgccg cagtaccagt tcgtcgccga cacgaaagtg
     2281 cggatcggct gattctccag acccgggccc gcgcccgggt tttgcgcttt ccgcgctagc
     2341 gattgagaca ttcagaacag agtgaacgac atgagcggac cctcaacata cgtccccaag
     2401 agcgccattg gccgctggtt cgagagccgg cttccggtca tgggcctcat ccacagttcc
     2461 ttcgtggcct acccgacgcc caagaacctg aactatttct ggactttcgg cggcatcctc
     2521 tcgctgatgc tcggcgtgca gatcatcacc ggcgtcgtgc tggcgat
//
LOCUS       DATIMH010000355         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5280198, whole genome shotgun sequence.
ACCESSION   DATIMH010000355 DATIMH010000000
VERSION     DATIMH010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5280198"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..2225)
                     /locus_tag="VLI90_06150"
     CDS             complement(84..2225)
                     /locus_tag="VLI90_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13821.1"
                     /translation="MRKPDITAFAIIALLAASSRAQPAPTTAAADVPVKEVVLFSSGV
                     GYFEHFGTVRGDGSTELRFKTNQINDILKSLVLQDVDGGQVTTVTYPSQDPIAKTLKS
                     FQVDITTNPPLADLLNQLRGAKVSIDNFGGEHIDGTILGVEKKKRLVNDHANETIEKS
                     YLNLLSGGGNIQPVELDGIRSIKLEDPQLQEELSKALLALASARDQDKKPVRINFTGQ
                     GERHIRIGYVVETPIWKTSYRLILGEPKKQAATTTQPTLADGKLQGWAIVENQTDNDW
                     NNVQLSLVSGRPISFIENLYTPLYIPRPVVQPELYASLRPQTYAGGMAEDKAVSDLGA
                     ITGAPAATAAPQQMAANRALRKELASRADQAQGNASLFSNITPAAPPMDPTASVATLA
                     SATNVGELFQYTVGNVSLPRQRSAMIPIITDPVEVERVSIYNASVLPRNPLNGARVKN
                     TTQKHLLAGPITVIDGASYAGDAQIDNLPPGQERLLSYGVDLQMLVDAKDNSSDSQLM
                     TGKIVKGVLTLTRKNVRSQDYVAENKSDHDKVLIVEHPREQSWNLVDTPKPMETTDNV
                     YRFKGKVAAGEKTKLTVKEQIIQVEEVAILPADIGQIEVYQRAGEIPKAVKDALAKAM
                     QLKQAMVDTQRQIDEAKAKLAGITAEQTRIRENMRTVDKGSQYYTRLMTKLNDQETQI
                     EKTQGDIDTLQKGLDGQRKELEAYLQDLNVA"
     gene            2396..3703
                     /gene="aroA"
                     /locus_tag="VLI90_06155"
     CDS             2396..3703
                     /gene="aroA"
                     /locus_tag="VLI90_06155"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HSV13822.1"
                     /translation="MTLTPINKPFRATIAPPGSKSLTNRALVLAALAEGISELSNVLF
                     ADDTLVMLDGLAKLGFQLEIDRDAHRVIVHGRGGQIGATDADLFCGNSGTTIRFLAAM
                     CALGHGEYTLDGVPRMRQRPIRDLVDLLRNLGVRVQYLGEDGFPPLKILADGLPGGIL
                     QYSSTVSSQYLSAVLMASPYARHEVRVDLVGEQTSWPYVAMTMQLMDRFGHTPELLRD
                     PSTGRPKQIVVPTGRYAATQYNVEPDASNATYFLAAAAVQPGSKVTIEGLGKGSLQGD
                     VGFADVLRRMGADLIFGRDFITIGGTDVLDGIEVDLRDMPDTAQTLAVVALFARGETI
                     IHGLHTLRVKETDRLAALSNELQKFGAAVTIDADDTLTIDPPKLLRRAEVDTYDDHRM
                     AMSFAIAGTRIEHVMIRNAECVNKTYPEFFADLEKLRTELRPA"
BASE COUNT          625 a         1208 c         1134 g          757 t
ORIGIN      
        1 ctgaccactg accactgaca acgaagcatc cgggtcgtcg cgagtacgct ggcgaacccg
       61 gcgtgggggg aatttaatcc accctacgcc acgttcaggt cctgcaagta cgcctccagt
      121 tccttgcgct gcccatccag ccccttctgc agcgtgtcga tgtccccctg cgtcttttcg
      181 atctgcgttt cctggtcgtt cagcttggtc atcagccggg tgtaatactg gctgcccttg
      241 tccaccgtgc gcatgttttc gcggatgcgg gtttgctcgg cggtgatgcc ggcgagcttg
      301 gccttcgcct cgtcgatctg ccgctgcgta tccaccatcg cttgcttcag ctgcatcgcc
      361 ttggccagcg cgtccttcac cgccttgggg atttcaccgg cgcgctggta gacctcgatt
      421 tgcccgatgt ccgccggcag gatcgcgacc tcttcaacct gaatgatctg ctccttcacc
      481 gtcagcttgg tcttctcccc cgcggcgact ttgcctttga agcgatatac gttgtcagtc
      541 gtctccatcg gcttgggcgt atcgaccaga ttccaactct gctcgcgcgg gtgctcgacg
      601 atcagcacct tgtcgtggtc cgacttgttc tcggccacgt aatcctgcga ccgcacgttc
      661 ttccgcgtca gcgtgagcac gcccttgacg atcttgccgg tcatcagctg gctgtcgctg
      721 ctgttgtcct tggcgtcaac gagcatctgc agatcgacgc cgtaactgag caatcgttcc
      781 tgccccggcg ggagattgtc aatctgcgca tccccggcgt aggacgcgcc gtcgatgacg
      841 gtgatcggcc cggccagcag gtgcttctgc gtcgtgttct tcacccgcgc gccgttgagc
      901 ggattccgtg gcaggaccga cgcgttgtag atcgagacgc gttcgacttc gaccggatcg
      961 gtgatgatcg ggatcatcgc cgaccgttgc cgcggcagcg aaacgttacc gacggtgtac
     1021 tggaacaatt cgccgacatt agtggccgac gcgagggtcg ccaccgatgc ggtgggatcc
     1081 atcggcggcg ctgccggcgt gatgttcgaa aaaaggctcg cgttcccctg cgcctgatcc
     1141 gcgcggctcg cgagttcctt ccgcagcgcg cgattcgccg ccatctgctg cggcgccgcc
     1201 gtggcggcgg gcgcgccagt gatagcgccg aggtcgctga cggctttgtc ctctgccatc
     1261 ccccccgcgt acgtctgcgg ccgcaggctc gcgtacagct ccggttgcac caccggccgc
     1321 gggatgtaca gcggcgtata caggttctcg atgaacgaga tcggccgccc gctcaccagc
     1381 gacagctgca cgttgttcca gtcgttgtcc gtctgattct cgacaatcgc ccagccctgc
     1441 agcttgccat ccgccagcgt cggttgcgtg gttgtcgcgg cttgcttctt cggctcgcca
     1501 aggatcaaac gatagctggt cttccaaatc ggcgtttcga cgacataccc gatgcgaatg
     1561 tgccgttcgc cctggccggt gaagttgatg cgcaccggct tcttgtcctg gtcgcgcgcc
     1621 gacgccagcg ccagcagcgc cttgctcaac tcctcctgca gctgcggatc ttccagcttg
     1681 atcgagcgaa tcccgtccag ctcgaccggc tgaatgttcc cgccgccgct cagcaggttc
     1741 aggtacgact tctcgatcgt ctcattcgca tgatcgttca ccaggcgctt ctttttttcg
     1801 acgccaagga tggtgccgtc gatgtgctcg ccgccaaagt tatcgatcga caccttcgcg
     1861 ccgcgcagct ggttcagcaa atccgccagc ggcggattgg tcgtgatgtc cacctgaaaa
     1921 ctcttgagcg tcttggcgat cggatcctga gacggatacg tgacggtggt cacctgcccg
     1981 ccatcgacgt cctgcagcac gaggctcttg aggatgtcgt tgatctggtt ggtcttgaag
     2041 cgcagctcgg tgctgccgtc gccgcgcacg gtaccgaagt gctcgaagta acccacgccg
     2101 gaggagaaga gcacgacttc cttaaccgga acatccgccg ccgcggtggt gggcgcgggc
     2161 tgggcgcggc tggatgcagc cagtaacgcg atgatagcga atgcagtgat gtcgggcttg
     2221 cgcatgcgat agctcctttg agccgttcgg gagagaaatt gtaccgatga ttggacgcct
     2281 tcgcgggtaa aaggttcccg ccgcttgcgg cttcgcaaac ccgggccgcg tcgtttgcga
     2341 agccgcaagc ggcaaaatgc acggccgtcc actatactgc gcaacgatga gcgacgtgac
     2401 gttaacgcca atcaataaac ccttccgcgc gacgatcgcc ccgccggggt cgaagagctt
     2461 gaccaaccgc gcgctcgtcc tggcggcctt ggcagagggg atcagcgaac tgtccaatgt
     2521 tctcttcgcc gacgacaccc tcgtcatgct cgatggtttg gcgaagctcg gtttccaact
     2581 ggagattgac cgcgatgccc accgcgtcat cgttcacggc cgcggtggac aaatcggcgc
     2641 aaccgacgct gatcttttct gcggcaacag cggcacgacg atccgcttcc tcgccgcgat
     2701 gtgcgcgctc ggccacggcg aatacacgct cgacggcgtg ccgcgcatgc ggcaacggcc
     2761 tatacgcgat ctggtcgatc tcttgcgcaa ccttggcgtg cgcgtccaat acctcggcga
     2821 agatggcttc ccgccgctga agatccttgc cgacggcctg cccggcggca tcctgcagta
     2881 cagctcgacc gtctcgtcac agtatttgtc agccgtattg atggccagcc cctacgcgcg
     2941 gcacgaggtg cgcgtcgatc tggtcggcga acaaacgagc tggccgtacg tcgcgatgac
     3001 gatgcagctg atggaccggt tcggtcacac accggagctg ctgcgcgatc catcgactgg
     3061 ccggccgaaa cagatcgtcg tgccaaccgg gcgatacgcg gcgacgcaat acaacgtcga
     3121 gcccgacgcc agcaacgcga cgtacttcct cgccgccgcc gcggttcagc cggggtcgaa
     3181 ggtcacgatc gaaggcctcg gcaaaggtag cctgcagggc gatgtcggct tcgccgacgt
     3241 gctgcgccgc atgggcgccg acctgatctt cggtcgcgac ttcatcacga tcggcggcac
     3301 cgacgtcctc gacggcatcg aggtcgatct gcgcgacatg cccgacaccg cgcagacgct
     3361 ggccgtcgtc gcgctgttcg cacgaggcga aacgatcatc cacggcctgc acaccctgcg
     3421 cgtaaaagag acggatcgac tggccgcgct gtccaacgaa ctgcagaagt tcggtgcggc
     3481 ggtcacgatc gacgcggacg acacgctgac gatcgacccg ccgaaacttt tgcggcgggc
     3541 ggaagtggat acctatgacg atcatcgcat ggcgatgagc tttgcgatcg cggggacgcg
     3601 gattgagcat gtgatgattc gcaacgcgga atgcgtgaac aagacgtatc cggagttttt
     3661 cgcggacttg gagaaacttc ggacggagtt aaggcccgcg tagcggtcgc cccgaagggg
     3721 cacg
//
LOCUS       DATIMH010000356         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4122476, whole genome shotgun sequence.
ACCESSION   DATIMH010000356 DATIMH010000000
VERSION     DATIMH010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4122476"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VLI90_06160"
     CDS             <1..189
                     /locus_tag="VLI90_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13823.1"
                     /translation="EKLNNLKNQFSAAVHEERWRDAINIGDTISRDFPNSRIALEVKD
                     MMETLHKRASRQAEVAKA"
     gene            254..1360
                     /locus_tag="VLI90_06165"
     CDS             254..1360
                     /locus_tag="VLI90_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSV13824.1"
                     /translation="MPDVLIIGAGPAGCVAASLLARARFSVHLLEQHTFPRDKVCGEC
                     LSALGIDVLNRAGLESRIAALHPARFTRALLYADQTPAIEVPLPRAMWGVSRSALDAA
                     LLDAARDAGAIVTQPARGERVTPGPRPRAHWRCLRTNQQREESFNWVLIGDGKGALTL
                     GSAIRGASGDLGIKAHFTHVASPRDAIELFGVRGHYGGVAAVEGDRWNVAFSVPVERV
                     RQSGGNVEQMFSDMTAENAALRAAFGSARRASDWLASPLPRFAVRPAWPHRIIPLGNA
                     AAALEPVGGEGMGLAMRSAELAAAALIAHRDGGEESSMRHLRRQFDQLWRARSWGSRW
                     LARMLSSPTLADAAAPLVAGNRHVIRAILRMTGK"
     gene            1730..1999
                     /locus_tag="VLI90_06170"
     CDS             1730..1999
                     /locus_tag="VLI90_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13825.1"
                     /translation="MHEVTINIGWAVIFSVVGGVVGILLVLCSSLILPKLIDRLTPDL
                     DEAKEIARGNQAVAEYFGRVVAAGVIGISIVVGAAVLGGILAALH"
     gene            2006..>2643
                     /locus_tag="VLI90_06175"
     CDS             2006..>2643
                     /locus_tag="VLI90_06175"
                     /inference="COORDINATES: protein motif:HMM:NF019227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HSV13826.1"
                     /translation="MRTFHVILAALTLLISATLALARGGGGCFEAGTPVLTPCGPRAI
                     EQLKPGDAVFSVVANQLCAARVSSCQQVTPAQYIEIQLDDRRLHVTAEHPFETSRGTF
                     VEAHDLHAGDEIECVERDVAHARRIASVRIVAAVHPAYNLLVTPGGTFVADGAVVHNK
                     GCFLPDTPILRADGSEAPIASIKPGDELLAFSEDGTPTHAIVRDIITHDVDE"
BASE COUNT          450 a          903 c          857 g          433 t
ORIGIN      
        1 gaaaagctga acaacctgaa gaaccagttc agcgccgcgg tgcacgaaga gcgctggcgc
       61 gatgcgatca acatcggcga cacgatctcg cgcgacttcc cgaacagccg gatcgcgctg
      121 gaagtgaagg acatgatgga aacgctgcac aagcgcgcga gccgccaggc ggaagtggcg
      181 aaggcgtgaa gtcactggga aaagtcccat cggcccgaca cgcttaagcg ggtcgcggcc
      241 tgtatctgat gcaatgccag acgtgctgat catcggcgca ggccccgccg gctgcgtcgc
      301 ggcttcgctg ctggctcgcg ctcggttctc ggttcatctc ctcgaacagc acacgtttcc
      361 ccgcgacaaa gtctgcggcg aatgtctcag cgcgctgggc atcgacgtgc tgaatcgcgc
      421 gggattggaa tcgcgcattg ccgcgcttca tcccgcccgc ttcacgcgcg cgttgctcta
      481 cgccgatcaa acacccgcga ttgaagtccc gctcccgcgg gcgatgtggg gcgtgtcacg
      541 atctgcattg gacgccgcgt tgctggacgc cgcgcgcgac gcgggcgcga tcgtcacaca
      601 acccgcgcgc ggcgagcgcg tgacgccggg gccgcggccg cgcgcgcact ggcgctgtct
      661 gcgaacaaac cagcagcgcg aagaatcctt caactgggtc ctcatcggcg acggcaaagg
      721 cgcgctaacg cttggcagcg caatccgcgg cgccagcggt gatctcggca tcaaagccca
      781 cttcacccac gtcgccagcc cgcgcgacgc gattgaactc ttcggtgtcc ggggccacta
      841 cggcggcgtc gccgccgtcg aaggcgaccg ctggaacgtc gcgttcagtg tccccgtcga
      901 acgcgtccgc caatccggcg gaaacgtcga acagatgttc agcgacatga ccgcggaaaa
      961 cgccgccctt cgcgccgcct tcggcagcgc ccggcgagcg agcgattggc tcgcatcccc
     1021 cctgccgcgc ttcgcggtgc gtccggcgtg gccacaccgg atcatcccat tgggaaacgc
     1081 cgccgccgca ctggaaccgg tcggcggcga aggtatgggc ttggcaatgc gaagcgcgga
     1141 actcgcggcg gcggcgctga tcgcgcaccg tgatggcggc gaggaatcat cgatgcgcca
     1201 tcttcgccgg cagttcgacc agctgtggcg cgcgcgcagc tggggcagcc gatggctggc
     1261 gcggatgctg tcgagtccaa cacttgccga cgccgcagcg ccgctggtcg cgggcaaccg
     1321 acacgtgatc cgcgcgatcc tgaggatgac tggaaagtaa gaacctacca cgggggcgcg
     1381 ccagacacgc aagcgtgtta tggaggccag cacctctccg cgcctctgcg cgcctctgcg
     1441 cggtacacgt tgaactctct tcctcggcgc cctggcggcc ttggccctga tccgcgtccc
     1501 aacctcgcca cgccgggttc ggaggtactc cgacgacccg gatgctcctg gcgcgcgaaa
     1561 gaatccgggt cgtcgcgagt acgctccgaa cccggcgtgg ttcagttttg accacttctg
     1621 caagagcccc cttacaaatc cgccgccgcc gcgtaattgt cggcgtcatg gtgaacgtct
     1681 acactgccgg ctcaacacgc cgctcgtcat gggcgcaagg gaaactcaca tgcatgaagt
     1741 gaccatcaac atcggctggg ccgtgatttt ctcggtggtc ggcggcgtcg tcggcatcct
     1801 gctcgtcttg tgcagctcgc tgatcctgcc caagctgatc gatcgtctca cgcccgacct
     1861 ggacgaagcg aaggagatcg cccgcgggaa tcaggcggtg gcggagtatt tcggccgcgt
     1921 cgtcgccgcg ggggtgatcg gcatcagcat cgtcgtcggc gccgcggttc ttggcggcat
     1981 cctcgccgcc ctccactgaa cgttcatgcg gacgttccac gtcattctcg ctgcgctgac
     2041 gttgctgatc agcgcgaccc tcgcgctcgc ccgcggcggt ggtgggtgct ttgaagccgg
     2101 cacgcccgtg ctgacgccgt gcgggccgcg cgcgatcgag cagctcaagc ctggcgacgc
     2161 ggtgttcagc gtcgtcgcca atcagctctg cgctgcgcgc gtctcttcat gccagcaggt
     2221 cacgcccgcg cagtacatcg agatccaact cgacgaccgc cggctgcacg tgacggcgga
     2281 acatccattc gaaacttcgc ggggaacatt cgtcgaagcg cacgaccttc acgccggcga
     2341 tgagatcgag tgcgtcgagc gcgacgtcgc tcacgcgcgg cgcatcgcca gcgtgcgcat
     2401 cgtggccgcc gtgcatccgg catacaacct gctcgtcacg ccgggcggga cgtttgtcgc
     2461 tgatggcgcg gtcgttcaca acaagggctg cttcctgccg gacacgccga tcctgcgggc
     2521 cgacggcagc gaagcgccga tcgcgtcgat caaacccggc gatgagctgc tggcgttcag
     2581 cgaagacggc acgccgacgc acgcgatcgt gcgcgacatc atcacgcacg atgtggatga
     2641 gta
//
LOCUS       DATIMH010000357         4066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_82124, whole genome shotgun sequence.
ACCESSION   DATIMH010000357 DATIMH010000000
VERSION     DATIMH010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82124"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /gene="ald"
                     /locus_tag="VLI90_06180"
     CDS             complement(<1..983)
                     /gene="ald"
                     /locus_tag="VLI90_06180"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HSV13827.1"
                     /translation="MRIGVPKEVKADEYRVAMMPVGADVLVRAGHEVFIETNAGIGSG
                     FADEDYAKVGAKIVLSHEQVFASSDMIVKVKEPQPQEIGLFHAGQVVFTYFHFAASRE
                     LTEACRKSGITAIAYETIKDRAGRLPLLTPMSEIAGKMSIQEGAKYLEKPMMGRGILL
                     GGVPGVPPANVVVLGAGIVGTNAAKVAAGLGANVVLMDVNLERLRYLDDVMPANVSTI
                     FSDPHTVRDHLRQADLVVGAVLIPGATAPRLVTRKDLVEMKNGSVIVDVAIDQGGCVE
                     TARPTTHKEPTYVVEGVVHYCVTNMPGAVGRTSTIALCNATLPYAVKIANKG"
     gene            1363..>4066
                     /locus_tag="VLI90_06185"
     CDS             1363..>4066
                     /locus_tag="VLI90_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13828.1"
                     /translation="MQIAGKSSGRNARRACASCALLTVAALTGRASGQTWVGNTQDWN
                     TASNWSPAVVPNSTSAAAVFPSFGSGLSTVNISASVSAQSLSFTNTSGQYLISGGANQ
                     TLSGVTTINVSAGGTGAVGIGLTSVATGNLLFPSGGNLTINNNNANSGSFLVFGSGSV
                     IGTPGTGGVVFNGAGLTSFIGSFAALGSPNNMVVGGITANGPGEVVLGGDGTNLGGSL
                     TLNGGLMQLDYVPTTATKIASSNPLNLNGGTLQFFTGNGVITQNFSTTNVGAGDTDVQ
                     GIINTGGTGNVTFGAGAINHSVAGTVDFQPQSGVLAITTSTGNTGGLLGTGPAFATVN
                     GGANWAMALGGNVTAVTTYGTDNYASGVNTDVTTSTTKFSGITTNSLRFNTNAPTVLT
                     LTGNNTIQSGGILVTPNFTDANGAQITGGSINATANGELLFHVYGSALEIFSNLVSTA
                     GLTKTGPGLLLLEASSPGLTGPININRGSLEVLVNTSAVNSASQINFNDNRLNSVQQM
                     TVDLGFGASGTISRPIRVAAPGGLSGPEDFGTLFTTGNSVNSTVTLSGAISSPAGLNT
                     PICFQALDGSSQFNLTNSGNSFSGNVILKQGSLGISSNGTLGAGGNTLYLDTLGNGGL
                     VFLNSGVTLTHAMTINTETPIVCNGTDSNTISGAITSFLNSGIDKQGTGTLTLTGSTT
                     ASLGNFNVDGGTLALTGAAVGVASGVNVFVQTGGTFNAGSAQSQHYGFIDLLGGTFTA
                     TSAGPGFIFVNQILDTAGGSINLGNAGSLVLALQGSGAGISVSGNSTWVSPGNSATIG
                     NLTGTPAPISIKPGVTLTNGIALSGVSGFTVTGGGTLFQNSDATNVLNMTAPITVGAA
                     STFRVADASSHSGLGNFGTGAFTLDGGTFEFSGAPVTTSKSIAIT"
BASE COUNT          784 a         1371 c         1157 g          754 t
ORIGIN      
        1 cctttgttcg cgatcttcac tgcgtacggc agcgtcgcgt tgcacagggc gatcgtgctc
       61 gtgcggccca cggcccccgg catgttcgtc acgcaataat gcacgacgcc ctcgacaacg
      121 tacgtcggct ccttgtgcgt cgtcggccgg gccgtctcga cgcagccgcc ctggtcgatc
      181 gcgacgtcga cgatcacgct gccgttcttc atctcgacca ggtccttgcg cgtgaccagc
      241 cgcggcgccg tcgcgccggg gatcaacacc gcgccgacga cgagatcggc ctgtcgcaga
      301 tgatcgcgca cggtgtgcgg atcgctgaag attgttgaga cgttcgccgg catcacgtcg
      361 tcgagatacc gcaaccgctc gaggttcacg tccatcagca cgacgttcgc ccccagcccc
      421 gcggcaacct tcgccgcgtt ggtgccgacg atgcccgccc ccagcacgac gacgttcgcc
      481 ggcgggacgc cgggaacgcc acccagcagg atgccgcggc ccatcatcgg tttctccagg
      541 tacttcgccc cttcttgaat gctcatcttc cccgcgatct cactcatcgg cgtgagcagc
      601 ggcaatcgac ccgcgcgatc tttgatcgtc tcgtaagcga tggcggtgat gccgctcttg
      661 cggcaggctt cagtcagctc ccggctcgcc gcgaaatgga aataggtgaa caccacctgc
      721 cccgcatgga acagcccgat ctcctgcggc tgcggttcct tcaccttcac gatcatgtcg
      781 ctgctggcga acacctgctc gtgcgacagc acgatcttcg cgccgacttt cgcgtagtct
      841 tcatccgcga acccgctgcc gatccccgcg ttggtttcga tgaaaacctc atgccccgcc
      901 cgcaccagca catcggcgcc gaccggcatc atcgccacgc gatattcatc cgccttcact
      961 tccttgggaa caccgatgcg catttaatga ttcctccagt cgcatcatct tagaactgat
     1021 gtgccgacac gtatacacgt cgacaacctg ttcagcgtgc gtcacgactg ttggtgcata
     1081 gatggcgtcc atgtccgcac ctgttccccc gctggagaat ttcgttgact tgcgccgaat
     1141 ggcccctgac aatcccgctg ccgcaagaat gaaaaatgct gctggaagtc actcgcgtgg
     1201 gtgagtaatg aagcagctcg cacgaccacg tcgcggcatc ggtcgtacta atcagcgcac
     1261 gggcgcgaac gaacgcgcgt ccgagggaac gccgcaccac atcagcggcg aacacaatgt
     1321 cgaaccgcac ggttcggcgg cacgcgggcg agtgggtgat tcatgcagat tgcagggaag
     1381 agcagcgggc gcaacgcccg ccgggcgtgt gcgtcatgcg cgctgctgac cgtggcggcg
     1441 ttgaccggtc gcgcttctgg acaaacttgg gtgggcaaca cgcaggattg gaacaccgcg
     1501 tccaactgga gcccggcggt cgtgccgaac tccacgtcgg ccgccgccgt ctttccttcc
     1561 ttcggttccg gcctgagcac cgtcaatatc tctgccagcg tctcggccca gtcgctgtcg
     1621 ttcactaata ccagcggcca gtacttgatc agcggtggcg ccaatcaaac cttgagcggc
     1681 gtgaccacga tcaacgtctc ggcgggtgga accggagccg ttggcatcgg cctgaccagc
     1741 gttgcaacgg gcaacctgct gtttccttcc ggcggcaacc tgaccatcaa caacaacaac
     1801 gccaacagcg gttcgtttct ggtctttgga tccggctccg taattggaac acccggtact
     1861 gggggcgtgg tgtttaacgg ggcagggctc acttccttca tcggatcgtt cgccgccctt
     1921 ggatcgccca acaatatggt tgtcggcggg atcacggcga acggaccggg cgaggtggtc
     1981 ttgggcggtg acggcaccaa tctcggtggc agcctgaccc tcaacggcgg tttgatgcag
     2041 ttggattacg tccccaccac cgcgaccaag atcgccagca gcaacccgct gaacctgaac
     2101 ggcggcacgc tgcagttctt caccgggaac ggtgtcatca cgcagaattt ctccaccacc
     2161 aacgtcggcg ccggcgacac cgatgtccaa ggcattatca acactggcgg cacaggcaac
     2221 gtgacgtttg gggcgggagc gatcaaccac agcgtggcgg gaaccgtcga tttccaaccg
     2281 cagagcggcg ttctggccat caccacatcc accggcaaca ccggcggcct gctcggcacc
     2341 gggccggctt tcgccacggt caacggcggc gccaattggg cgatggcctt gggcggcaac
     2401 gtcaccgccg tcacgaccta cggcacagac aattacgctt ccggcgtgaa caccgacgtc
     2461 acaacgtcca ccacgaagtt ctccggcatc accaccaact cgcttcgctt caacaccaac
     2521 gcgccaaccg tcctcacgct gaccggcaac aacacgatcc aatccggcgg catcctcgtc
     2581 acgccgaact tcaccgatgc caacggcgcg cagatcaccg gtggaagcat caacgccacc
     2641 gccaacggcg agctgctgtt ccacgtgtac ggttctgcgc tggagatctt ttccaatctc
     2701 gtttcgaccg cgggcctgac caagaccggg ccgggtttgc tgctgttgga agcgagcagc
     2761 ccgggactga ccggcccgat caacatcaat cgcggaagcc tggaggtgct ggtcaatacg
     2821 tcggcggtga attccgccag ccagatcaac ttcaacgaca accgcctgaa cagcgttcag
     2881 cagatgacgg tggacctggg cttcggcgcg agcggtacca tcagccggcc cattcgcgtt
     2941 gccgcgcccg gtggcctcag tggtccggaa gatttcggca ctttgttcac gaccggcaac
     3001 agcgtcaact ccacggtaac gcttagcggc gcaatctcgt cgccggctgg cctgaacacg
     3061 ccgatctgct tccaggccct tgatggcagc agccagttca acttgaccaa cagcggcaac
     3121 agcttttccg ggaatgtcat cctgaagcag ggatcgctcg gcatcagttc caatggaacc
     3181 ttgggcgcag gcggaaacac gctgtatctc gacacacttg gcaatggcgg gctggtgttc
     3241 ctcaactccg gtgtgacgct aacgcatgcc atgactatca atactgagac acccatcgtc
     3301 tgcaatggca cggacagcaa taccatctca ggcgcgatta ccagcttctt aaacagcggc
     3361 atcgacaagc aaggcaccgg aacgctcacg ctcacgggat cgactaccgc gtccttgggc
     3421 aactttaacg tagatggagg aaccctggcc ctcaccggcg cggcggtggg cgttgcgtca
     3481 ggtgtgaacg tcttcgtgca aacaggcggg acatttaacg ccggctcggc ccaaagccaa
     3541 cactacggct ttatcgacct gctcggaggc acgttcaccg caaccagcgc cggtccgggt
     3601 ttcatctttg tcaatcagat cctagacact gccggtggct caatcaatct tggcaatgca
     3661 ggctcgctcg tgctggcact ccaaggcagc ggtgccggca tttccgtcag cggcaactcc
     3721 acctgggtga gccccggcaa tagcgccacg atcggcaatc tcaccggcac acccgcgcca
     3781 atatcgatca agcctggcgt gacgttgacc aacggcatcg ccttgagcgg cgtatccggc
     3841 tttaccgtga ccggcggcgg tacgctcttt caaaactccg atgccacgaa cgtactcaac
     3901 atgaccgcgc cgattacggt gggcgcggcc agcacgttcc gcgtggccga tgcctccagc
     3961 cattccggtc tcggtaactt cggcaccggg gcgtttaccc tcgacggcgg cacgtttgaa
     4021 ttcagcggcg cacccgtgac gacgtccaag tccatcgcca tcaccg
//
LOCUS       DATIMH010000358         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4704299, whole genome shotgun sequence.
ACCESSION   DATIMH010000358 DATIMH010000000
VERSION     DATIMH010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4704299"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1537)
                     /locus_tag="VLI90_06190"
     CDS             complement(<1..>1537)
                     /locus_tag="VLI90_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13829.1"
                     /translation="FHVYPPNFKGAKIEPSFEGLMCAYNLTCAGTDWATAGRPRVRAG
                     DTILVHAGLYKYNRFEYTNDPAVNRTVPLDGTYYLTADGTPERPIVIAAAGDGEVIFD
                     GGGNFALFDVKAADYNYFEGITFRNADIAIWGGTQFAIGSKGLTVKRCRFENVGAGIF
                     TNYSGSSNYYIADNWFIGRDDPNHVIGWAGNFWQPFNGVDGQKFPPVMGSYVAVKVYG
                     PGHVIAYNYVANFHDGIDVETYGNPDGSAAEGGPSYPPKAYWDRRPVAIDFYNNYMTN
                     FHDNPFEADGGMHNVRVMRNMMINSASHAMCNQPSIGGPTYWIRNIEYHLPGGSTRLT
                     GGSAGVLFYNNTILSETAAQGASNVHWRNNLMLGENSAPAIFSINTYTNYSSSDYNGF
                     RPNPGAAASFQWNSPPANVAADFTLVTAGPQGAAAAAGAGRGAGAPATRRLEQRSFAT
                     LAEYSAATKQDQHSALVDYDIFVNVPRLDAQDRARVQTVYKAEDFDFSLRPGSAAIDR
                     GTPL"
BASE COUNT          257 a          474 c          501 g          305 t
ORIGIN      
        1 gcagtggtgt cccgcgatcg attgccgccg atccaggccg cagcgagaaa tcgaaatctt
       61 ctgccttgta caccgtctgc acgcgggcgc ggtcctgcgc gtcgagccgc ggcacgttga
      121 cgaagatgtc gtaatcgacg agcgcgctgt gctgatcctg cttggtcgcc gcgctgtatt
      181 ccgccagcgt ggcgaagctg cgctgctcca gccgccgcgt cgccggcgcc cccgccccgc
      241 gccctgcgcc cgctgcggcc gcagcacctt gagggccggc ggtgaccagc gtgaaatcgg
      301 cggcgacgtt tgccggcggc gaattccatt ggaacgacgc ggcggccccc gggttcggtc
      361 ggaacccgtt gtagtcggac gaggaatagt tggtgtacgt attgatgctg aagatcgcgg
      421 gcgcgctgtt ctccccgagc atcagattgt tgcgccagtg cacgttgctc gcaccctgcg
      481 ccgccgtctc ggacaggatc gtgttgttgt aaaagaggac accggcggag ccgccggtca
      541 gccgggtcga tccaccgggc aagtggtact cgatattccg gatccagtac gtcggcccgc
      601 cgattgacgg ctgattgcac atcgcgtgcg acgcggagtt gatcatcatg ttccgcatca
      661 cgcgcacgtt gtgcatcccg ccgtcagcct cgaacgggtt gtcgtgaaaa ttggtcatgt
      721 agttgttgta gaagtcgatc gcgaccggcc gccgatccca gtacgccttc ggcgggtacg
      781 acggcccgcc ttccgcggct gacccgtcgg gattgccata ggtttcgacg tcgatcccat
      841 cgtggaaatt ggccacatag ttgtacgcga tgacgtggcc ggggccgtac accttgaccg
      901 ccacgtacga ccccatcacc ggcggaaact tctggccgtc gacaccgttg aacggctgcc
      961 agaaatttcc cgcccagccg atgacgtgat tgggatcgtc gcggccgatg aaccagttgt
     1021 cggcgatgta gtagttgctg gagcccgaat agttggtgaa gatgccggcg ccgacgttct
     1081 cgaagcggca gcgtttgacc gtcaaccctt tcgatccgat ggcaaactgc gtcccgcccc
     1141 agatcgcaat gtcggcattg cgaaaggtga tcccctcgaa gtagttgtag tcggccgcct
     1201 tgacgtcgaa cagcgcgaag ttgccgccac cgtcgaagat cacctctccg tcgccagcgg
     1261 cggcgatgac aatcggtcgc tccggcgtgc cgtcagccgt caggtagtag gtgccatcga
     1321 gcggtacggt tcgattgacc gccgggtcgt tggtgtattc gaaccggttg tatttgtaga
     1381 ggccggcgtg gacgaggatc gtgtcgccgg ctcggacgcg tgggcggccg gccgtggccc
     1441 agtcggtgcc ggcgcacgtc aggttatagg cgcacatcag cccttcgaac gacggctcga
     1501 tcttcgcgcc tttgaagttt ggcggataga cgtgaaa
//
LOCUS       DATIMH010000359         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2969155, whole genome shotgun sequence.
ACCESSION   DATIMH010000359 DATIMH010000000
VERSION     DATIMH010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2969155"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VLI90_06195"
     CDS             complement(<1..512)
                     /locus_tag="VLI90_06195"
                     /inference="COORDINATES: protein motif:HMM:NF020544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HSV13830.1"
                     /translation="MSVTVTYYRLPAQERESVTRDQSAWEQFRRRIQSAHFQSFHSAA
                     AGLDGFSGSREERYAKLAALLQEHRDPRRFDMEKDWHIVGYLLTGRAEMIEEHRPDDL
                     LHSVIFGGHNVSVITGYGAVRYYDGQLVTQLADALRAADRKSVSRRFEPARMRELDIY
                     APTDEGEREAV"
     gene            complement(613..804)
                     /locus_tag="VLI90_06200"
     CDS             complement(613..804)
                     /locus_tag="VLI90_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13831.1"
                     /translation="MSHLDPAWLLGADIAGCIVLFFAFRALFRERRRMRQRMAEHVER
                     LVRVEGKLDRILASRTTGG"
     gene            complement(1392..3047)
                     /locus_tag="VLI90_06205"
     CDS             complement(1392..3047)
                     /locus_tag="VLI90_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13832.1"
                     /translation="MHTSLEVPALVVTTRRTANLRRVLARTAFVVALLVAAFSAAFAG
                     LRLREVAFLDTAEFHFKGDVRNGFRWGMAAQDEGLLNLYDRIQAELDGGGPRRTLDYT
                     PLRLTAVWLWTRWANRHFPGYTQWVDDYDFTRPMLNANTIAEAASAVLVFALIWLWTR
                     RMDDARRPPGEPRRYLRGAVAGLFGALLFWFNPAVIWVSHCWPQWDVWLMPFFLAAVL
                     LASVNGWFTAGLMLAIGAHLKGQILLGAPILILWPLVSLRFGAVLRLLAGFLFAGTLV
                     ALPWFKIYAPALGDAPAAWNHPAVIWCVIAGLVLTAAAVVGIVLLRRYHKPIGWIAPL
                     YAAMWALIFLATLKYFHADTAWYTVGFKYGTEKYGDMMAGNGVWNIAKMQQTYAFNGV
                     SWNDPSDPLALPLINKVVTYRAATFIVYGVCLLICAIGAALHSHRRRPDTRFLAAIAA
                     PWVCFFVVLTQMHGRYLMWGAGMCSLLAGVSPGMALLGVLVSVISTLGTLENQLDFAP
                     SYAPDWRRDLQAVDPHVGWMLLLIAMILLYVAVAPSRGTVMKR"
     gene            complement(3211..>3399)
                     /locus_tag="VLI90_06210"
     CDS             complement(3211..>3399)
                     /locus_tag="VLI90_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13833.1"
                     /translation="MRVTKVLGVESIGGGSVKAANSWEQPDVVVPVVGNASVVEGRLR
                     VVVPAPGLSVLRASVTRA"
BASE COUNT          676 a         1170 c         1004 g          549 t
ORIGIN      
        1 acagcctcgc gctcgccttc gtccgttggg gcgtaaatgt ccaactccct catccttgcc
       61 ggttcaaacc gccggcttac gctcttgcga tctgctgctc gtagtgcgtc ggctaactgt
      121 gtgacgagct ggccatcata gtaacgaact gcgccgtatc cggtgatgac tgaaacgttg
      181 tggcctccga agataacgct gtgaagcaag tcgtccggcc ggtgctcctc gatcatctcg
      241 gctcgtcccg tgagcagata tccgacgatg tgccaatcct tctccatatc gaatcgtcgc
      301 ggatcgcgat gctcctgaag caacgctgcg agcttcgcat atcgctcttc ccggctgccg
      361 ctaaacccgt caaggcccgc ggcggcggag tgaaatgact ggaagtgcgc gctctgaatg
      421 cgccgccgaa actgctccca tgccgattga tcgcgcgtca cactctcccg ttcttgcgcg
      481 ggcaggcggt agtaggtgac agtgacactc atgtgtgaag tcggctaacg caccaacatt
      541 cactggccga ggccgacgac cagcgttcgc cttacaaggt ccgttgtggc cccggtccag
      601 tgcagtgttt gattatccgc cggtcgtgcg gctggcgaga attcgatcca gcttaccctc
      661 tacccgtacc aaacgttcaa cgtgttctgc catgcgctga cgcattcggc ggcgttcacg
      721 gaagagcgcg cgaaacgcaa agaagagaac gatgcacccg gcgatgtctg cgccgagcag
      781 ccacgccgga tcgagatggc tcatgtgcgg actcccgtag gcgataacta gtattcccgc
      841 gcgcgccgtg ccatatgcaa cggcgccgcg tcgcgggggc ttgtcgaagc tatattctac
      901 ctgccaggca gttgtcggca acgcagcagt gtgcgccgat ccgcgcaggc gaccttctga
      961 tcagggttct tcgcggcata caccaacggc ggccaaatgg cgagacggcc gtctcacccc
     1021 gctctataaa tcactgtgtc atttagcgac ggtcggcggc gcagcgctgg ctgtcgcgcc
     1081 ggcgtcgatc agcaattgga cgcacgctgc gctaaccacg ccgggcagag agccgccaga
     1141 cctaaaaaaa cccgatcagc gccgggagca ccccaatgac cattccccac tcacccccat
     1201 tgttcgaccg tgtttcccag acgcggaggg cgagtgatgc gaccgacatg gcgatcgccg
     1261 cgatcacggc tccgagcgct atccaagtac ggcacgagaa cacgttttgc gggagaagcg
     1321 gttgttggtc agtcataact gaatgacctt tcgcattcat gaagctttac ccgagcggcg
     1381 cacgcgcgcg atcaacgctt catcaccgtc ccacgcgaag gtgccaccgc gacgtacaac
     1441 agaatcatcg cgatcagcag cagcatccag cccacgtgcg ggtccaccgc ctgcaggtcg
     1501 cgccgccaat ccggcgcgta ggatggcgcg aaatccagct ggttctccag cgtgcccagc
     1561 gtcgaaatca ccgacaccaa tacgcccagc agcgccattc cggggctgac gccggccagc
     1621 agcgagcaca tgcccgcgcc ccacatcagg tatcgcccgt gcatctgcgt cagcacgacg
     1681 aagaagcaga cccacggcgc cgcgatcgcc gccaggaagc gcgtgtctgg ccgccgccgg
     1741 tggctgtgca gcgcggcgcc gatcgcgcag atcagcaagc agacgccgta gacgatgaac
     1801 gtcgccgctc gatacgtcac gaccttgttg atcagcggta gcgccaacgg gtcgctcggg
     1861 tcgttccacg acacgccgtt gaatgcgtac gtctgctgca tcttcgcgat gttccacacg
     1921 ccgttgccgg ccatcatgtc gccgtacttc tcggtgccgt atttgaaccc gaccgtgtac
     1981 cacgcggtgt cggcgtggaa gtatttgagc gtcgccagga agatcagcgc ccacatcgcc
     2041 gcgtacagcg gcgcgatcca gccgatcggc ttgtgatatc gccgcagcag gacgatgccg
     2101 acgacagcgg cggccgtcag cacgaggccg gcgatgacgc accagatgac cgccggatga
     2161 ttccacgccg ccggcgcatc gcccagcgcg ggcgcgtaaa tcttgaacca cggcagcgcc
     2221 accagcgtgc ccgcgaagag gaacccggcc agcagtcgaa gcaccgcgcc gaaccgcagc
     2281 gacaccagcg gccacaggat caggatcggc gcgccgagca aaatctgccc tttgagatgc
     2341 gcgccgatcg ccagcatcaa ccccgcggtg aaccatccgt tgactgacgc cagcagcacc
     2401 gccgccagga agaacggcat cagccaaacg tcccactgcg gccagcaatg gctgacccag
     2461 atgacggccg ggttgaacca gaacagcagc gcgccaaaca gccccgcgac ggcaccgcgc
     2521 aaatagcgcc gcggctcgcc cggcggccgc cgcgcgtcat ccatccgccg ggtccacagc
     2581 cagatgagcg cgaagacgag gaccgccgac gccgcctcgg cgatcgtgtt cgcgttcagc
     2641 atcggccggg tgaagtcgta atcatcaacc cactgcgtgt accccgggaa atgccgattc
     2701 gcccagcgcg tccagagcca gaccgccgtc aagcgcagcg gcgtgtaatc cagcgtccgt
     2761 cgcggccccc cgccgtccag ctccgcctgg atgcggtcat acaggttgag caggccttcg
     2821 tcctgagcgg ccatgcccca gcgaaagccg ttgcgcacgt cgcccttgaa gtgaaactca
     2881 gcggtatcga gaaacgcgac ttcgcgcaag cgaagccccg cgaatgcggc tgagaaagct
     2941 gcgacgagca gcgcgacgac gaatgcggtg cgcgccagca cccgccgcag gttcgctgtg
     3001 cgccgagtag tgacaaccag cgccggcact tcaagtgacg tgtgcacagc cagcgatcat
     3061 ccgggcgacg cgcggcattt tcaatcgggt aatcaaaacg cgcgcgacga gcattccatg
     3121 atagccgggg gcggaagccc cggtctgttc gaacctaaaa agaccgggcc ttccggcccc
     3181 ggctataacg gagaaaatca ttcttcgaga ctaggcgcgc gtcacactcg cgcgcagcac
     3241 gctcaacccc ggcgccggca ccacgacgcg cagccgccct tccaccacgc tggcgttccc
     3301 caccaccggc accacgacgt ccggttgctc ccagctgttc gcggccttca cgctgccgcc
     3361 gccgattgat tcgacgccca gcaccttggt tacgcgcat
//
LOCUS       DATIMH010000360         7982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_82665, whole genome shotgun sequence.
ACCESSION   DATIMH010000360 DATIMH010000000
VERSION     DATIMH010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82665"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VLI90_06215"
     CDS             complement(<1..356)
                     /locus_tag="VLI90_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13834.1"
                     /translation="MKKSLLAFSLSIAALLAPVLAQSPKSTATGLDALDENRVMTELA
                     NRGMETLLKREFEVNKVPPGQQSGVLTLVSIRRLQDPSLRLTPTEKQDLLEKISKGIV
                     SALPSISDPTALMQIA"
     gene            complement(473..937)
                     /locus_tag="VLI90_06220"
     CDS             complement(473..937)
                     /locus_tag="VLI90_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSV13835.1"
                     /translation="MRIRGAKAVHYDSGPNMTPLVDVVMVILIFLMLAGSFGGVEHYL
                     VSSTPVTKTGGGANTSVVPDEVQYTITVDSPMGLSDRFVVRAGDLVTSDMDKLTAALV
                     RKRKEFNAAGTATDKVQVIVAPGRTVKYKFLIDVYQAALSAEFTKVSFQQAH"
     gene            complement(1033..1524)
                     /locus_tag="VLI90_06225"
     CDS             complement(1033..1524)
                     /locus_tag="VLI90_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSV13836.1"
                     /translation="MKKVHIPETHVAHPNVTPLIDIVMCLIIFYMLVAKIGVTTGAEK
                     MDIPESIVGTDIKDMGNTLTLNVRPGAIEEPLVTALVKLPGSAISRQSELKLRDPASG
                     QNQLLNTLKMYRQQNDQFKVIIRGEADMPYRFLEPVLVACADANVRNVNFNTKKVSEK
                     TGG"
     gene            complement(1568..2440)
                     /locus_tag="VLI90_06230"
     CDS             complement(1568..2440)
                     /locus_tag="VLI90_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSV13837.1"
                     /translation="MPVAKRSSYSYSKRRIALLVAMMAATAFTVASTSFGQSTGTPPP
                     ASGDQPTGLLHLILGHMDFVFITIAGLSVWGLTLIIQASIKNRASVFMPEETTNQIRE
                     MISQRKFKELIDFTETDDSFVSKALNPALKRAPSFSSMKEAMETAIGEHTAEQFRKIE
                     YLNIIGNLGPLLGLLGTVLGMIEAFQAVQQTGGQADVAKLSGGISTALTHTFLGLFLA
                     IPCLAAFGVLRTIVDRLTVRGALTAEELLLMIKPAEAAKPSVGGAAPRIAASPQPGVA
                     RKPLPTPAPSAAST"
     gene            complement(2489..3643)
                     /locus_tag="VLI90_06235"
     CDS             complement(2489..3643)
                     /locus_tag="VLI90_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13838.1"
                     /translation="MSVPTPPAPPAEATSAAGGAKMAAPPAPPQPPPVPEGMLGDAAA
                     AQPPPVEYYREQQSFWRQPWVQNVLPLATSLALHLSLIVIGFATYKTVQKVVQVVKEQ
                     IIIPESTMADNGPPGGIQHPGLGADPTRDAAQDLVKDASQDAGWATKATDELNQTLAG
                     GAASDADSSLISIGANASTGGKGNGIGGGGADGGQLAPFGVPGGGAGMGPKSNFAGTG
                     GNAHQIMYFCDASGSMLSVFTQLKLELKRSIDALKPVQSFNVVFYRDDQYYALSKDGL
                     VMASPDNKRKAYEFIDSQVSTGSTQPIPAIKFALSQKPQLMYLLTDGFDQIANFDDVI
                     NTFKNGNTEKKIHVNCIFLKSNDDPNLERVLRQIATENGGLMKIIAKSEF"
     gene            complement(3893..5119)
                     /locus_tag="VLI90_06240"
     CDS             complement(3893..5119)
                     /locus_tag="VLI90_06240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV13839.1"
                     /translation="MTFRCVGADELDRVADTRFLCYGGAERDRARFHERLRNDARAKP
                     GDYLLAEVDGRAVGTATSLSLKMWVRGGAIPCQGVAWVGAIKTFRRRGGGEGSPGVAS
                     AVMHEMLRMARERGDVVTALMPFRGSYYEHFGYSLVERRNEWTIPLSVLPAGPFDGVR
                     FYEPADFTARAECLRRVNQRGQSDIERTDDVWRSFATPALDGLEVVDRPSPDGPVHGS
                     MFFTQQHENGKDILRVVEAVYEDLPALRRQLYFLASLKDQYSAVKIALPGDLRLNWLL
                     KETQLPHRPVNHAFATVNPITRMQLRVLNHAKFLEALHLPGDVTGSVTVAVHESEGSV
                     SRLRIDIEGGRASVGARDGATEFECPDRVWAAVATGDLPATEALRLGLATGDMRAAGV
                     LDVLSRGPAPWSNEYF"
     gene            5184..5771
                     /locus_tag="VLI90_06245"
     CDS             5184..5771
                     /locus_tag="VLI90_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HSV13840.1"
                     /translation="MARIAILVEKIYEDLELQYPRLRLREAGHTVDIVGPKAGETYIG
                     KHGYPQKSERAASEVKASDYALIVIPGGSSPDHMRRSGDMVRLVRDAAAAKIPIAAIC
                     HGPWMLCSTDALRGRRCTSYMSIVHDVVNAGGKWVDEECVVDGPIITARTPDDLPAFS
                     NAILQLVSQGKAQGVKGKPIAPAWVMETIPAASRG"
     gene            5804..6139
                     /locus_tag="VLI90_06250"
     CDS             5804..6139
                     /locus_tag="VLI90_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13841.1"
                     /translation="MLICGGYDVCSRRNLRLHAVGTDPTHGHYLVSDKDFLDWELVRD
                     KLKNLLSLFLGRATGNAGRRWFSDGASRKRVETQSHFDHLVNSYLPDQCGIFWKPGVM
                     LPEIPDGIL"
     gene            complement(6190..6951)
                     /locus_tag="VLI90_06255"
     CDS             complement(6190..6951)
                     /locus_tag="VLI90_06255"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSV13842.1"
                     /translation="MFRLTREVRFAINAGGDDQLDRAPSNSYGGFPSIVGLGVYYTAR
                     VTLRGELGEQSQYLVNIKDVDDAVRQYLREAKLSLADPAAHPPRIVREIYAAVAARWP
                     NEVARVALALSPLLALSLDRSEFPMMSLSQKFEFAASHRLHNPSLGEAANRKLFGKCN
                     NPNGHGHNYELQVTVRGNESLADLPALERIVASSVIDRVDHKNLNIEVPEFRELNPTV
                     ENIAKVIHGWLKPQVENLASVTVWETAKTWCEYGE"
     gene            complement(6979..>7982)
                     /locus_tag="VLI90_06260"
     CDS             complement(6979..>7982)
                     /locus_tag="VLI90_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13843.1"
                     /translation="EGVPAPVADLFALHAEHGPFDGFWQDVQIYRAYLCEPELLYALG
                     QLASREGHPMAGLAAQAAGLAAGGVSPARHTQVGGFLTTQGWSAAAERELSAAIWLSG
                     GEEINAYFQLARLAAERGDELAVAQRLEEALNGINRRGGGTVMSRTTRFGDVLPWTAE
                     DAWAEVHWHYLRAAQAANDLPGVQKHLAELTKLDRLGQIVHKDPGLASDIVPALQETG
                     QSADAQKCFDAAYTDLHQAMVEDPTNAEAKNNLAWLCARCGQRLDEAVRLADEAVAAA
                     PNNSAYLDTAAEAHFRNGDTKGAVGLESRAIELRPGDAFMKGQLERFEHGGAGGASR"
BASE COUNT         1477 a         2701 c         2284 g         1520 t
ORIGIN      
        1 ttggcgatct gcatcagcgc cgtgggatcg ctgatgctcg gcagcgcgct gacgatcccc
       61 ttggaaatct tctcgagcag gtcctgtttt tccgttggcg tgaggcgaag gctggggtcc
      121 tgcagccggc ggatcgacac aagcgtgagc acgccgctct gctggccggg ggggactttg
      181 ttcacttcga attcgcgctt gagcagcgtt tccatcccgc ggttggccag ctcggtcatc
      241 acgcggtttt cgtcgagcgc gtccagaccg gtggcggtgg atttcggcga ctgcgcgagg
      301 acgggggcga ggagcgcggc gatcgaaagc gagaacgcga ggagggattt cttcatgatg
      361 aacgggatcg tctgtgattg cgatgggcgt tgcgcgagcc ggcgttcacc tacttctcgg
      421 ctagatcctt cgactccggc agagtaccgc ctgcgctccg gatgacaccc tgttaatgcg
      481 cctgctgaaa agaaaccttc gtgaactccg ccgacaacgc ggcctggtat acgtcgatca
      541 ggaacttgta tttcacggtc cgccccggcg cgacgatcac ctgcaccttg tccgtcgcgg
      601 tgccggcggc gttgaactcc ttgcgcttgc ggaccagcgc cgctgtcagc ttgtccatgt
      661 cgctggtgac caggtcgccc gcgcggacga caaagcgatc gctcaagccc atcggcgaat
      721 cgaccgtgat cgtgtactgc acttcatccg gcaccacgct ggtattcgcc ccgccgccgg
      781 tcttggtgac cggcgtgctg ctgaccaggt aatgctcgac ccccccgaag ctgccggcca
      841 gcatcaggaa gatcaggatg accatgacaa cgtccaccag cggggtcata ttgggaccgc
      901 tgtcgtagtg gacggctttt gcgcctcgaa ttctcatggg ggatcagttg ctggttactg
      961 gttgtaagtt gctagtgaaa gtcactttcg ttgcatttac tagcaacaag caactagcaa
     1021 ccagcaactc attcatccac cggtcttctc actgaccttc ttcgtattga agttgacgtt
     1081 gcgcacgttc gcgtccgcgc aggcgaccag cacgggttcc aggaatcggt acggcatatc
     1141 cgcttcgccg cggatgatga ccttgaactg gtcgttttgc tggcggtaca tcttcaaggt
     1201 gttcagcagc tggttctgcc ccgacgcggg gtcgcgcaat ttcaattccg attgccgcga
     1261 tatcgcgctg ccgggcagct tcaccagcgc ggtcaccaat ggctcctcga tggcgccggg
     1321 ccgcacgttc agcgtcagcg tgttgcccat atccttgatg tcggttccaa cgatgctctc
     1381 ggggatatcc atcttctccg cgccggtggt cacgccgatc ttcgccacca gcatgtaaaa
     1441 gatgatcaga cacatcacga tgtcgatcag cggcgtcacg tttgggtgcg cgacgtgagt
     1501 ttcaggaatg tggacctttt tcatcgcagt agatcccgcg cgcggaggac actcacatca
     1561 cgttgattca agtggatgcc gccgacggcg ccggcgtggg cagtggtttg cgcgccacac
     1621 ccggttgcgg tgacgccgcg attcgcgggg ccgctccacc cacgctgggc ttggccgcct
     1681 cggcgggctt gatcatcagc agcagttcct ccgccgtcag cgcgccgcgc accgtcaatc
     1741 gatcgacgat cgtccgcagc acgccgaacg ccgccagaca gggaatcgcc agaaacaatc
     1801 ccaggaatgt gtgcgtcagc gcggtcgaaa taccacccga gagcttcgcg acgtcagcct
     1861 gtccaccggt ttgctgcacc gcttgaaagg cttcaatcat ccccagcacc gtgccgagca
     1921 gcccgagcag tggaccgagg ttgccgatga tgttcaggta ttcaatcttg cggaactgct
     1981 cggccgtgtg ctcgccgatc gccgtctcca tcgcttcctt catgctgctg aagctcgggg
     2041 cgcgcttgag cgccgggttc aacgccttgg agacgaagct gtcgtcggtc tcggtgaagt
     2101 cgatcagctc cttgaacttc cgctggctga tcatctcgcg aatttgattg gtcgtctctt
     2161 ccggcatgaa gaccgatgca cggttcttga tcgacgcctg gatgatcagc gtcagtcccc
     2221 acaccgacaa cccggcaatc gtgatgaaca cgaaatccat gtggccgagg atcaggtgca
     2281 gcagcccggt cggctggtcg ccactggccg gcggtggtgt gccggtggat tgaccgaagc
     2341 tggtgctggc cacggtgaat gcggtggccg ccatcatggc caccagcagc gcgatccgcc
     2401 gcttgctgta cgagtaggac gaacgcttgg caacaggcat ggaactctcc aattcactcg
     2461 ctcgggtgag gccagtgttt tccaaatctc aaaattcgct cttggcgatg atcttcatca
     2521 acccgccgtt ctccgtcgca atctgccgga gcacgcgctc gaggttcggg tcatcgttgg
     2581 acttcaaaaa gatgcagttc acgtggatct tcttttccgt attcccgttc ttgaacgtgt
     2641 tgatcacatc atcgaaattg gcgatctgat cgaacccatc ggtcaagaga tacatcagct
     2701 gcggcttctg cgacagcgcg aacttgatcg cgggaatcgg ctgcgtgctg ccggtcgaga
     2761 cctggctgtc gatgaactca tacgccttgc gcttgttgtc cgggctggcc atcaccaggc
     2821 cgtccttgct cagcgcgtaa tactggtcgt cgcgatagaa gacgacgttg aacgactgca
     2881 ccggcttgag cgcgtcgatg gaccgcttca actccagctt cagctgcgta aagacgctga
     2941 gcatcgaacc cgacgcgtcg cagaaataca tgatctggtg tgcgttgccg cccgtgccgg
     3001 caaaattgct tttgggaccc atgcccgccc caccgccggg gacaccgaac ggcgccaatt
     3061 gtccgccgtc ggctccacca ccgccgatgc cgttgccttt gccgccggtg ctggcgttgg
     3121 cgccgatcga gatcaagctc gaatccgcgt cgctggcggc gccgccggcg agcgtctggt
     3181 tcagttcatc ggtagccttg gtcgcccaac ccgcatcctg cgatgcatcc ttgaccagat
     3241 cctgcgccgc atcgcgcgtc gggtcggcgc ccagaccggg gtgctgaatg ccacccggcg
     3301 ggccgttgtc ggccatcgtc gattcgggaa tgatgatctg ctctttgacg acctgcacga
     3361 ccttctgcac cgttttgtag gtcgcgaacc caatgacgat cagcgacagg tgcagcgcca
     3421 gcgaagtcgc caacggcagg acgttttgca cccacggctg ccgccagaac gattgctgct
     3481 cgcggtaata ctcgaccggc ggcggttgcg cggcagcggc gtcgcccagc atgccctcgg
     3541 gcacgggcgg cggttgcggt ggcgcgggtg gcgcggccat cttcgcgccg cctgccgcgg
     3601 acgtcgcttc agcaggaggt gcggggggcg tcggcacgga catcatcaac tccgttgtcc
     3661 aatcggtttg gcaacacgca cggcgggcga gccccgtctc ggctcgatac agtcaggcaa
     3721 gaagaagccg cggcgacaac ttaaatcccg cgcgggccgg gactaccacc gacacaatga
     3781 taatacgccg cttcggccgc tttcaaacaa aaaatgaagg gcttgccgat cggtgcatct
     3841 ttgcattttc cgctaaaaac acaagaaaga gacgtctcgt ctgacgcgat cgctagaaat
     3901 attcgttgct ccacggtgcc ggaccgcgcg acagcacgtc caatacgccc gccgctcgca
     3961 tgtcgcccgt tgccaagccc aaccgtagcg cttctgtcgc cggtagatcg cccgtcgcaa
     4021 cggccgccca gacacgatcg ggacattcga attctgtcgc cccgtcgcgc gcgccgacgc
     4081 ttgctcgccc gccttcgatg tcgatgcgaa gccggctcac agagccttcg ctctcatgca
     4141 ccgccacggt caccgatccg gttacgtcgc cgggcaggtg caacgcttcc aggaacttcg
     4201 cgtgattcaa aacccgcagc tgcatccgcg tgatcgggtt cacggtcgca aaggcgtgat
     4261 tcaccggccg gtgcggcagc tgcgtttcct tcagcaacca gttcagccgc agatcgcccg
     4321 gcagtgcgat cttcacagcc gaatattgat ccttcaggct cgccaggaaa taaagttgcc
     4381 gccgcagcgc cggcaggtct tcgtagacgg cttcgacaac gcggaggata tccttgccgt
     4441 tctcgtgctg ctgcgtgaag aacatcgacc catgcaccgg cccatcgggc gacggccgat
     4501 cgacgacctc caacccatcc agcgccggcg tcgcaaacga ccgccagaca tcgtcggtgc
     4561 gctcgatgtc agactgcccg cgctggttca cccgtcgcag acactccgcc cgcgcggtga
     4621 aatccgcagg ctcataaaac cgcacaccat cgaacggccc cgccggcagc accgacagcg
     4681 ggatcgtcca ttcgttccgc cgctcgacca agctatatcc aaagtgttcg tagtaagagc
     4741 cacgaaatgg catgagtgcg gtgacgacgt cgccacgctc gcgcgccatc cgcagcatct
     4801 cgtgcataac tgcgctggcc acgcccggcg aaccctcgcc gccgcctcgt cgccggaatg
     4861 ttttgatcgc ccccacccac gcaacgcctt ggcacgggat ggcgccgccg cgcacccaca
     4921 tcttcaatga caagctggtc gccgtgccga ccgcacggcc atccacttcc gccaacagat
     4981 aatcccccgg cttggcgcgg gcatcgttcc gcaagcgctc gtgaaaccgc gcgcgatctc
     5041 gctccgcccc gccgtagcaa aggaaccgcg tgtccgccac gcgatccagc tcatccgcac
     5101 ccacgcatcg aaacgtcatc gccatgatgc ccgaagctta tctttccagt tgccatttac
     5161 catttgccct ttacgattcg actatggcac gaatcgcaat cctcgtcgaa aagatttacg
     5221 aagacctcga gctgcaatac ccccgccttc gcctgcgcga agccggtcac acagtggaca
     5281 tcgtcggccc caaggccggc gaaacgtaca tcggcaagca cggctatccg caaaaaagcg
     5341 aacgcgccgc cagcgaagtc aaagccagcg actacgccct catcgtcatc cccggtggct
     5401 catcgccgga ccacatgcgc cgcagcggcg acatggtgcg gctcgtccgc gacgccgccg
     5461 ccgcgaagat ccccatcgcc gcgatctgcc acggcccgtg gatgctctgc tcaacggacg
     5521 ccctgcgcgg ccgccgctgc acgagctaca tgagcatcgt ccacgacgtc gtgaacgccg
     5581 gcggcaaatg ggtggacgaa gagtgcgtgg tcgatggccc gattatcaca gcaagaacgc
     5641 cggatgatct gccggcgttc agcaatgcga tcctgcaatt ggtcagccaa ggcaaagcgc
     5701 agggcgtgaa aggaaagccg atcgcgccgg cgtgggtgat ggagacgatc cccgcggcgt
     5761 cgcgggggta ggttatcgac gtccgatccc cgggcttcag cgtgtgctga tctgcggcgg
     5821 ctatgatgtt tgctcacgac gcaatttgcg attgcacgcc gttgggacgg atccgacgca
     5881 tggacactac ctcgtcagcg acaaggactt ccttgattgg gagctcgtac gcgacaagtt
     5941 gaaaaacctg cttagcctgt ttctaggtcg agcaacggga aacgcaggac gcagatggtt
     6001 ctccgacggc gccagccgca agcgcgtcga gacgcaatcg cacttcgatc acctggtgaa
     6061 ttcctatttg ccggaccagt gcggaatctt ctggaagcca ggagtcatgc ttcccgaaat
     6121 cccagacgga atcctttgaa cttgtcccca taatagccca gggcggaagc ccggggatcg
     6181 gacagaaaat cactccccat actcgcacca agtcttcgcc gtctcccaca ccgtcacgct
     6241 cgccaaattc tccacctgcg gcttgagcca gccgtggatc accttcgcga tgttctccac
     6301 cgtcgggttc aactcgcgga actccggcac ctcgatgttc agatttttat gatccacccg
     6361 atcgatcacg ctcgacgcca cgattcgctc cagcgccggc agatccgcca gcgattcatt
     6421 gccacgcacc gtgacctgca attcatagtt gtgcccatgc ccgttcgggt tgttgcactt
     6481 gccgaacagc ttacggttgg cggcttcacc cagcgaagga ttgtgcagcc ggtgcgacgc
     6541 ggcgaactcg aacttctgac tcaaactcat catgggaaac tccgatcgat ccagcgacaa
     6601 cgcaagcaac ggcgacaacg ccagcgcaac ccgcgccacc tcgttcggcc aacgcgccgc
     6661 caccgcggca taaatctcgc gcacgatccg cggcggatgc gccgccggat ccgccagcga
     6721 cagcttcgct tcgcgcaggt actgccgcac cgcgtcgtcc acgtctttga tgttcaccag
     6781 gtactggctc tgctcgccga gttcgccgcg caacgtcacg cgcgcggtgt agtatacgcc
     6841 caagccaacg atggacggga accccccata gctgttagat ggcgcgcgat ccagctgatc
     6901 atcgcccccc gcattgatcg cgaaccgcac ttcacgcgtc agtcgaaaca tacaataagt
     6961 atacccgggc taaccgcttc agcggctagc tccccccgca cccccgtgtt cgaaccgctc
     7021 caactgcccc ttcataaacg cgtccccagg ccgcagctcg atcgcccgcg actcgaggcc
     7081 caccgccccc ttcgtgtcgc cattgcgaaa atgcgcctcc gccgccgtgt ccaaataagc
     7141 cgaattgttc ggcgccgccg ccaccgcttc atccgccagc cgcaccgctt catcgagccg
     7201 ttgtccgcag cgcgcgcata gccaggcgag gttgttcttc gcttcggcgt tggttggatc
     7261 ttctaccatc gcctggtgaa gatccgtgta cgccgcgtcg aagcatttct gggcgtcggc
     7321 tgactgcccc gtttcctgaa gcgcagggac aatgtccgac gccagcccgg ggtccttgtg
     7381 cacgatctgc cccagccgat ccagcttggt cagctccgcc agatgcttct gcacccccgg
     7441 caaatcgttc gccgcctggg cggcccgcag gtaatgccaa tgcacctccg cccacgcgtc
     7501 ctcggccgtc cacggcagca cgtcaccaaa gcgcgtcgtg cgcgacatga ccgtgccgcc
     7561 cccgcgacga ttgatgccat tcagcgcctc ctccaatcgc tgcgccaccg ccagttcatc
     7621 gccgcgctcc gcggccaatc gcgccagttg aaagtacgcg ttgatctcct cgccgccgga
     7681 taaccagatg gcggccgaca gctcgcgctc ggccgccgca ctccacccct gcgtcgtcag
     7741 aaatccgccc acctgcgtgt gccgcgccgg cgacactccg cccgccgcca gccccgctgc
     7801 ttgcgccgcc aagcccgcca tcggatgccc ctcgcgcgac gccaactgtc cgagcgcata
     7861 cagcaattcc ggctcgcaca aatacgcccg atagatctgc acgtcctgcc agaacccgtc
     7921 gaacggccca tgctccgcgt gcagcgcgaa caggtccgcc accggcgccg gcacgccttc
     7981 gg
//
LOCUS       DATIMH010000361         4228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4123298, whole genome shotgun sequence.
ACCESSION   DATIMH010000361 DATIMH010000000
VERSION     DATIMH010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4123298"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..449
                     /locus_tag="VLI90_06265"
     CDS             54..449
                     /locus_tag="VLI90_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSV13844.1"
                     /translation="MEVDPNNPTPIYQQIVHRLCTAVAAGIYKPGDAIPSVRQQAVAL
                     LVNPNTVQRAYEELQRAGLLVARRGGAPIVAGAALAVALARTTDAVRNTFATGIADGA
                     AAGLPPSEIDRLYDQAWNGSRPREPEAAR"
     gene            446..1393
                     /locus_tag="VLI90_06270"
     CDS             446..1393
                     /locus_tag="VLI90_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV13845.1"
                     /translation="MNADHTPPAAIELINLTRRFGRTVAVDDVTLGIPAGTTCGFIGL
                     NGAGNTTTLRMLVGLLAPTAGAIRVAGCPMPAEHQLAKLRIGYVPDRPTVYSWMRVRE
                     AVAFCRSLYGPAWNQPRCDEVMKTLRLPPDRRVKHLSKGEGAKLSLLLAIGHDPEVLI
                     LDEPTGGFDVLARDEFLEGLLNVTTTQRDTGRARTVLFSSHALGDVQRLADSVAILHQ
                     GKLLLHRPLEQFLETTKRIRSVLEESTATVAPPGTVHQRISGREWIVTVKDFSPDQVE
                     FIRSKNHVQRIDVMNVTLDEAFRDYVRGEESPETEAVMS"
     gene            1390..2061
                     /locus_tag="VLI90_06275"
     CDS             1390..2061
                     /locus_tag="VLI90_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13846.1"
                     /translation="MKAGIVSLMRKDWRLYRLPVIACAMLGALGYVVSRITYLIDLRR
                     HYNFPFLWDYYLARAAWFGVFVTTIMAAAFGGAAFAAERRERWADFLNLLPPHRAVVA
                     LSKLLVAAVSLIVALQLHIAVLWFTGELRGNNWIGYMVIGCAMIMLFGTSWMLSTAIS
                     SAAVSASIAILFTVGAAVIVNSLGTTYGWQDDGAAIVFAAVSAITGTVRFVAGTIYYL
                     YRVQP"
     gene            2135..2818
                     /locus_tag="VLI90_06280"
     CDS             2135..2818
                     /locus_tag="VLI90_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSV13847.1"
                     /translation="MVNQLFPLIRKDWRLFRVPVLAVLILALMGYNVCFLLYRTDQRQ
                     FGQEAVLGQWWNQYMNDAAWFGLFTAVIMSAVFGGAAFATERRERWGDFLNLLPPGRA
                     LVIASKLLVSFATLAAIAAAHLLVLRMVGTLHHAGDWIGYMILGSGAAMLFGVSWMLS
                     TVLRSASLSASIAIALTIGAALVVAMVGTAFDWKNDWGYTVFTVLSAVTGVVCFGAGT
                     IYYLRRVEP"
     gene            2991..>4228
                     /locus_tag="VLI90_06285"
     CDS             2991..>4228
                     /locus_tag="VLI90_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13848.1"
                     /translation="MSEYGDDLALLWCVLLNAAVFGTAYRFAIRRMTRDRVQAALDAA
                     LAGYAIQYASICLPAVLGALRPSTVSCVAMLLSALLWAGAGRARKSAEELPLSRPEPK
                     WSSWVALIVACFAAGYALALARLQDVLPVMSNDALTYHFPAAVQWFQTGRLTLFPTWF
                     FNPANTYSPLGGSTFIAWLIAPFDNDVLARFVQVPALAIVGVALFQLCRQLGVRLAVA
                     ALIAAAGVLARPMLFASIMGKDDLFVAAFFLLALIAMSPPRAAERFATVRLGVVLGLL
                     VAMKYTALLSVPVLLLAIDGPWRAGWRWRWWSTAVGVAILVGGPWYWRNLWITGNPLF
                     PIDMTIAGRHVFRGLFTPARSEGLRSIAGIWNVLTGGDYGLVPVMTGICALGWLALLV
                     RQWRKSAPPTPGLRYAEDPDR"
BASE COUNT          667 a         1437 c         1298 g          826 t
ORIGIN      
        1 ctgcccaaaa acgcttgaca ttcgcgggac gactgtatta atgtattagt acagtggaag
       61 tggaccccaa caaccccacc cccatctatc agcagatcgt ccaccgcctc tgcaccgccg
      121 tcgccgccgg catctacaaa cccggcgacg cgatcccctc cgtccgccaa caagccgtcg
      181 cactgctcgt caaccccaac accgtccaac gcgcctacga agaactgcaa cgcgccggcc
      241 tgctcgtcgc ccgccgcggg ggcgcgccga tcgtcgctgg cgccgccctc gccgtcgcgc
      301 tggcgcgcac caccgatgcc gtccgcaaca ccttcgccac cggcatcgcc gacggcgctg
      361 ccgccggtct tccaccaagc gagatcgacc gcctgtacga ccaggcatgg aacggctcac
      421 gtccccgcga accggaggcc gcccgatgaa cgctgatcac actccacccg ccgcaattga
      481 attgatcaac ctcacccgcc gattcggccg caccgtcgcc gtcgacgatg tcaccctcgg
      541 cattcccgcc ggcacgacct gcggtttcat cggtctcaac ggcgccggca ataccaccac
      601 cttgcgcatg ctcgtcggcc tgctcgcgcc aacggccggc gccatccgtg tcgccggttg
      661 cccgatgccc gccgaacacc aactcgctaa gctccgcatc ggttacgtgc ccgatcgacc
      721 gaccgtctac agctggatgc gcgtgcggga agcggtcgcc ttctgccgct cgctatatgg
      781 cccggcatgg aaccagccgc gctgcgatga ggtgatgaaa acccttcgcc tcccgccgga
      841 ccggcgcgta aaacatcttt ccaaaggcga aggcgcgaag ctctcgctcc tgcttgcgat
      901 cggccacgac cccgaggtgc tgatcctcga cgagcccacc ggcgggttcg acgtgctggc
      961 gcgcgatgaa tttctcgaag gcctgctgaa cgtcacgacg acccagcgcg acaccggccg
     1021 cgcccgaacc gtcctgttct cctcccatgc cctgggtgat gtccaacgat tggccgactc
     1081 cgtcgcaatc ctccaccagg gaaaattgct gctgcaccgc ccgctcgagc agttcctgga
     1141 aaccaccaag cgcatccgca gcgtgctgga agaatccacc gccaccgtcg ccccgcccgg
     1201 cacggtgcac cagcgcatca gcggccgcga atggatcgtc accgttaaag atttctcgcc
     1261 cgatcaggtc gaattcatcc gcagcaaaaa ccacgtgcag cgcatcgacg tgatgaacgt
     1321 cacgctggac gaagccttcc gcgactacgt ccgtggcgag gagtcgccgg agactgaggc
     1381 ggtgatgtca tgaaagccgg aatcgtctcg ctgatgcgaa aggactggcg gctctatcgc
     1441 ctgccggtga tcgcgtgcgc gatgcttggc gctttgggat acgtcgtcag ccgaatcacc
     1501 tatctgatcg acctgcgccg gcattacaac ttcccgttcc tgtgggatta ctacctcgct
     1561 cgggcggcgt ggttcggcgt cttcgtcacc accatcatgg ccgcggcgtt cggtggcgcg
     1621 gccttcgccg ctgaacgtcg ggagcgctgg gccgactttc tgaatctgct gccgccgcat
     1681 cgagcggtgg ttgcgctcag caagctgctc gtcgcagccg tcagcttgat cgttgcgctt
     1741 cagctgcaca tcgcggttct ctggttcacc ggtgaactgc gtgggaacaa ttggataggc
     1801 tacatggtca tcggctgtgc gatgatcatg ctcttcggga cttcgtggat gctctcgacc
     1861 gcgatcagca gcgccgcggt gagcgccagc attgcgattt tgttcaccgt gggcgctgcg
     1921 gtcatcgtca actcgctcgg gacaacttat ggctggcaag acgacggggc cgccatcgtc
     1981 ttcgccgccg tcagcgccat caccggaacc gttcgcttcg tagccgggac gatctattac
     2041 ctgtaccgcg tgcagccgtg agttgcgccc aaccaacagg ccgcgtaaac gcggacgcta
     2101 aacgaggtgc gaaatcaacc ggggcgtaat catcatggtc aaccaactct tcccactgat
     2161 ccgcaaagat tggcggctgt ttcgcgtgcc ggttctggcc gtcctcatcc tggcgctcat
     2221 gggctacaac gtctgtttcc ttctctatcg aacggatcaa cgccagttcg ggcaggaggc
     2281 ggtgcttggt cagtggtgga atcagtatat gaacgatgcc gcctggttcg ggctcttcac
     2341 cgccgtcatc atgtcagcgg tcttcggtgg cgctgccttc gcgaccgagc ggcgggaacg
     2401 gtggggcgat ttcctcaacc tgctcccgcc aggccgcgct ttggtgatcg ccagcaaatt
     2461 gctcgtcagc ttcgccaccc tcgccgccat tgccgcggcg cacctattgg tccttcgaat
     2521 ggtcggcacc cttcaccatg ccggtgattg gatcggttac atgatcctcg gcagcggcgc
     2581 cgccatgctg ttcggcgtat cgtggatgct gtcaaccgtg ctgcgcagcg catcgctcag
     2641 cgccagcatc gcgatcgccc tcactatcgg cgccgcactg gtcgtcgcca tggtcggcac
     2701 cgcattcgat tggaagaatg actggggcta caccgtcttc accgtgctca gtgccgtcac
     2761 cggcgtcgtc tgcttcggcg ccggcacgat ctactacctg cgccgcgtcg agccgtgatt
     2821 gcggctgctc agtcggcaga ggtgacgtga aggtgataca tcccctcgct accaccactt
     2881 gcttgaccgg gctaacccct tcagcgattg ccttgggacg agcgtcccgc ggagccgcgg
     2941 gcttttattg tggtccttga tcgtcttgcg ctgaacccga caatgccgcc gtgtctgaat
     3001 atggcgatga cctggcgctg ctgtggtgcg tgctgctgaa tgccgccgtt tttggcaccg
     3061 cgtatcggtt tgccatccgg cggatgaccc gcgaccgcgt gcaggcggcg ctcgatgcgg
     3121 cgctggcggg gtacgccatc caatacgcca gcatctgtct gccggcggtg ctcggggcgc
     3181 tgcgaccgag cacggtcagc tgcgtggcga tgttgctgtc cgcactgctt tgggccgggg
     3241 ccggccgggc gcgcaagtcg gccgaggaac tgccactgtc tcgtcctgaa ccgaaatggt
     3301 catcgtgggt cgcgctgatc gtcgcgtgct tcgccgccgg ttatgcgctg gctttagccc
     3361 gattacagga cgtgctgccg gtgatgtcca acgatgcctt gacgtatcac ttccccgccg
     3421 ccgtgcagtg gtttcagacc gggcgattga cgctcttccc cacctggttc ttcaacccgg
     3481 ccaacaccta ctcgcccttg ggcggatcga cgttcatcgc ctggctcatc gcgccgtttg
     3541 ataacgacgt gctggcgcga ttcgtgcagg tgccggcgct ggcgatcgtc ggcgtcgcgc
     3601 tgttccaact ctgccggcag ctgggcgtgc ggctggcggt cgcggcgttg atcgccgccg
     3661 cgggggtgct cgcccggccg atgctgttcg cctcgatcat gggaaaggac gacctgttcg
     3721 tcgcggcgtt cttcctgctc gcgctgatcg cgatgtcgcc gccgcgcgcc gccgagcgct
     3781 tcgcaacggt gcgactcggc gtggtcctcg gcctgctcgt ggcgatgaag tacaccgcgc
     3841 tgctgagcgt gcccgtgctg ctgctggcga tcgacggccc ctggcgagcc ggctggcgct
     3901 ggcgatggtg gtcgacagcg gtcggcgtcg ccatcctcgt cggcgggccg tggtactggc
     3961 gtaatctgtg gatcacggga aacccgctgt ttccgatcga catgacgatc gccggccggc
     4021 atgtcttccg cgggctcttt acccccgcgc gaagcgaagg gctgcgatcg atcgccggga
     4081 tatggaacgt gctgaccggc ggggattacg ggttggtccc ggtgatgacg gggatctgcg
     4141 cgctaggatg gttagcgctg ctggtgcggc agtggagaaa aagcgcgccc ccgacgccgg
     4201 gtctgaggta cgccgaagac ccggatcg
//
LOCUS       DATIMH010000362         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_947893, whole genome shotgun sequence.
ACCESSION   DATIMH010000362 DATIMH010000000
VERSION     DATIMH010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947893"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..983
                     /locus_tag="VLI90_06290"
     CDS             216..983
                     /locus_tag="VLI90_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13849.1"
                     /translation="MIARRPFHPTLLALLIVALAADASLARMWVGGSGVHGWYYGGGD
                     THRGRYDDSASSDTGTGSNAAVAANDPNNGMNSPGAAGNDEAAHAAAAMDVARRLVEQ
                     RFESSPEWTEASMAFDEARAAFEQAKRRIIAALANDPRYQSAVRECVAINDALTALRA
                     SAAGQTSEQIIRTAAAAMRARAAVNAMESAACANDAAASAAAAKLKDATTVMAQLRQQ
                     EREAVYADAAWKSAQQTYQQSMGAMASARVTPSERMR"
     gene            complement(1224..1916)
                     /locus_tag="VLI90_06295"
     CDS             complement(1224..1916)
                     /locus_tag="VLI90_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HSV13850.1"
                     /translation="MMLAAVATAGAAGPSTEPSVPPMRIALVGDSTMIDRGGWGGAFA
                     DLLFDEDEVQCINLSRGGRSSSSFITEGRWAKCLELKPAYIFIQFGCNDEPGHGADRE
                     ANANTTYRRNMTRYVDEARAAGATPILVTSLARRQFGPDGKIHSTLEAYAATVRAIAQ
                     ERHVPLIDLHARSIELYEKLGPDGCRALSPKKADGSYDGTHLNATGAAAVASLVVDEL
                     NRALPELGAYFE"
BASE COUNT          387 a          787 c          707 g          383 t
ORIGIN      
        1 tcgtgtctcc ttcgccggtt ctcgatcgcg cgcggtgttt ggccagcagc gcggcgacgt
       61 gttcgcacac gttagcttcc acacgggtga tgtccagtct cacaaacgcc gctgccacgg
      121 cgggaaaagg agggaaaagg cggttgcaac attcccctgc gattgcgata attagaattg
      181 atctttcgac gtgcgcgcgg gaaggcatgt tcgccatgat cgcccgacga ccgttccatc
      241 cgactcttct cgctctgctc atcgtcgccc tcgcggccga cgcttcgctc gcgcgcatgt
      301 gggtcggcgg cagcggtgtg cacggttggt attacggcgg cggcgacacg caccgcgggc
      361 ggtatgacga ctccgccagc tccgacaccg gcaccggttc caacgccgcg gtggccgcca
      421 atgatcccaa caacggcatg aacagccccg gcgcggccgg caacgacgaa gccgcccacg
      481 ccgcggcggc gatggacgtt gcccgccggc tggtcgagca gcgctttgaa tcgtcgcccg
      541 agtggaccga agcgtcgatg gcgttcgacg aagcgcgagc ggcgttcgag caggcgaagc
      601 ggcgcatcat cgccgcgctg gcgaacgatc caagatatca atcggcggtt cgagagtgcg
      661 tggcaatcaa tgacgcgctg accgcgctgc gcgccagcgc cgcgggtcag acgtcggagc
      721 agatcatccg caccgccgcc gcggcgatgc gggcgcgggc ggcggtgaat gcgatggagt
      781 ccgccgcgtg cgcgaatgac gccgcggcca gcgccgccgc tgcgaagctg aaggacgcga
      841 cgaccgtcat ggcgcagctg cggcagcagg aacgcgaggc cgtctacgcc gacgcggctt
      901 ggaaatcggc gcagcaaacc taccagcaga gcatgggcgc gatggccagc gcgcgcgtca
      961 caccgtccga acgcatgcga tgactcttgg tcatcctcca gcgcaggcgt actcgccaga
     1021 cacgcctcgc gcgactcgcg gtgaatccgc ggaggggcaa gcgacttccg cacaatccgc
     1081 tcggaacgac acttcattta ccacgagctg gattcttcga ctgcgcccga acacgcgcgc
     1141 ttcgctcagg acgccgaata ccacgccggg ttcggagggt actcccgacg accctgattc
     1201 ttcccacgcg ccgtccaacc agctcactcg aagtacgccc ccagctccgg cagcgcgcga
     1261 ttcagctcat ccaccaccag actcgccacc gccgcggcgc cagtcgcgtt caagtgtgta
     1321 ccgtcgtaac tgccatccgc cttcttcggc gacagcgcgc gacagccatc cggtcccagc
     1381 ttctcgtaca gctcgatgct gcgcgcatgc agatcgatca gcggcacgtg ccgctcctgc
     1441 gcgatcgcgc gaaccgtcgc agcgtaggct tccagcgttg agtggatctt cccgtccggt
     1501 ccgaactgcc gccgggccag cgacgtgacg aggatcggcg tcgcccccgc cgcccgcgct
     1561 tcatcgacgt accgcgtcat gttccgccga tacgtcgtgt tcgcattcgc ctcgcgatcg
     1621 gcgccgtggc cgggttcgtc attgcagccg aattggatga agatgtacgc cggcttcagc
     1681 tcgaggcatt tcgcccatcg tccctcggtg atgaagctgc tggagctgcg accgccgcgg
     1741 gagagattga tgcactgcac ctcgtcttca tcgaagagca ggtccgcgaa tgccccgccc
     1801 caaccgccgc gatcgatcat ggtggaatcg ccgaccagtg cgatgcgcat cggcgggacc
     1861 gacggttcag ttgatggtcc ggctgcacca gcggtggcga cggccgcgag catcatggca
     1921 atgattacca ctggccgcga attgatcctc ttgtacatca ttcggccgct cacccggtgc
     1981 cgcgcgtgcg tggtcttcag tcacatctac cgggttgcgg cacgtgtgtt gagatcatct
     2041 tgtgcagcgc gcgcaattcc cgcgcggcca cttcgcgacg caatttcacg ttgctttgca
     2101 gttgcttcag aacccgatgc tcacgccgcc atcgaccggc agcaccacgc ccgtgacgaa
     2161 tttcgccgcc ggcgagcaca ggtacaccgc ggcccaaccg atgtcttccg gctggcccat
     2221 ctgacccatc ggcgtgcgcg ccaaaatccg cgccttgcgc ggcg
//
LOCUS       DATIMH010000363         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_948298, whole genome shotgun sequence.
ACCESSION   DATIMH010000363 DATIMH010000000
VERSION     DATIMH010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948298"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..796
                     /locus_tag="VLI90_06300"
     CDS             362..796
                     /locus_tag="VLI90_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13851.1"
                     /translation="MPSRGSALLPLLIVGSMLLVGCQPKPKPTPPPPPPPATMSDAGR
                     IQADAQQRDPHARAGLVVGVHLQDQNAAVSLVPVSQQPAADDPNRVKEGSVFTFMDSS
                     NTPIANGSVIYVEDGIFVIHYAPIEGGHAPAVGDIALHLSTR"
     gene            834..1610
                     /locus_tag="VLI90_06305"
     CDS             834..1610
                     /locus_tag="VLI90_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HSV13852.1"
                     /translation="MASPRLILASASPRRRELLHAAGYEFEVHPPDVDEDKQPPQILP
                     ADLAQRLAQLKAYTVAERFPNDIVLAADTVVAFGDRALGKPANDTAAVKMLELLSGTT
                     HIVITGVVIMHKAAGITRSARVMSAVRMKMLSSDEIRRYVEGGQWQGKAGGYGIQDER
                     DAFVTCIAGSHSNVVGLPMEKATELLAGVGIVPTQVKSESAADSSDQTRGKGDKETRG
                     EDDSLAAPPVPMSPPPPVPLSPQNESETKRLSPPAWDTLL"
     gene            1614..1925
                     /locus_tag="VLI90_06310"
     CDS             1614..1925
                     /locus_tag="VLI90_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13853.1"
                     /translation="MEIFGGIIALIVGVAGWYYLFYSQAATRLGAIEDAAANQRRHRL
                     RRTNGLVMLALAVLIYAGAHIESPRAFVMVWIAVLLLFFVFVALGFADIRLTAKLRRK
                     R"
     gene            1930..2403
                     /locus_tag="VLI90_06315"
     CDS             1930..2403
                     /locus_tag="VLI90_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSV13854.1"
                     /translation="MKRNFASPSLVHLLLLALLGGCGTGPATTQSSLPTVSMKIGART
                     FTLEVAADEYSRERGLMERDSMPADHGMIFVFPSDSSEGFWMKNTHIPLDILFIDAGG
                     KVVSVHTMKPFDMNTTSSDGEYRYAIELNAGAAADVGVKAGDHVDIPRNVGAASH"
     gene            2596..>3388
                     /locus_tag="VLI90_06320"
     CDS             2596..>3388
                     /locus_tag="VLI90_06320"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSV13855.1"
                     /translation="MNAAPATQPHLRLVRDDDPPRDGDLIAPAPAASIAGDAVGQVEA
                     LRTEITALTAELSLLRRKGETLNFHMSRLDEELRLAARLQQDFLPKSIPQIGRVHFHT
                     LFRPAGYVSGDFYDVMRLDEKNVGFYIADAVGHGVPAALLTMFIKHALATKQITKSGY
                     RLLGAGETMTRLNETLMEQNLSAATFATALYGTINVETLQLTFARGGHPHPILLRANG
                     EVEPLLSDGGLLGVFPGETFDERTVQLAQGDRLLLFTDGIEVIFSD"
BASE COUNT          631 a         1154 c         1017 g          586 t
ORIGIN      
        1 atcggtcaac agcatcaaca cctatttcaa catcgccccg caggaagctt acgacctgta
       61 cctggcggca cagctgctga tgaaggaccg ctttgaaccc cggctgaggg agatcgtgaa
      121 ggagatgaac gggagtaaat aaggcgccac tcgcgcgatt ggagccgctt gcggtttagc
      181 gtctgttcga atccgcaaca catgctaagc cgcaagcggc gggaaccagc gccttccatt
      241 ggggtgtctc caaccactcc cgcacttgcg taaactcacc cctttacgct ttactatgcg
      301 ccgcacacgc gctggcgcgc accgccttcc cggcgcgccg gtgcgcacaa tggagccctc
      361 aatgccttca cgtggatctg cgcttctgcc tttgctgatc gtcggttcga tgttgctggt
      421 cggctgccag ccgaaaccca aacccacccc gcccccgccg cccccgccgg cgacgatgag
      481 cgacgccggg cgcatccagg ccgacgccca gcagcgtgat ccgcacgccc gcgcggggct
      541 cgtcgtcggc gttcacctgc aagaccaaaa cgccgccgtc tcgctggtcc ccgtttcaca
      601 gcagccggcg gccgacgatc cgaatcgcgt caaggaaggc agcgtcttca cgttcatgga
      661 ttccagcaac acgccgatcg ccaatggctc ggtgatctac gtggaagatg gcatcttcgt
      721 gatccactac gcaccgatcg aaggcggcca tgccccggcg gtgggcgaca tcgccctgca
      781 cctttcgacc cggtaatcgc gagcttcgac gacgcgcgca agcgcgccac acattggcat
      841 ccccgcggct catcctcgcc agcgccagcc cgcgccgccg agagctgctg cacgcggccg
      901 gttacgaatt cgaagttcac ccgcccgacg ttgacgaaga caagcaaccc ccgcagattc
      961 ttcccgctga tctggcgcag cgcctggcgc agctgaaggc gtacaccgtc gccgagcgct
     1021 tccccaacga catcgtgctg gccgccgaca ccgtcgtcgc cttcggcgat cgcgcgctag
     1081 gcaaacccgc caacgacacc gccgcggtga agatgctcga actgctcagc ggcaccacgc
     1141 acatcgtcat caccggcgtc gtcatcatgc acaaggcggc gggcatcacg cggtcggcgc
     1201 gcgtgatgtc ggcggtccgc atgaagatgc tgtcgagcga cgaaatccgc cgctatgtcg
     1261 aaggcggaca gtggcagggc aaagccggcg gatatggcat tcaggacgag cgcgacgcgt
     1321 tcgtgacgtg catcgccggc agtcattcca acgtcgtcgg cctgccgatg gagaaggcga
     1381 cggagcttct ggcgggagtg gggattgtgc cgacgcaagt gaaatcggaa tccgcagctg
     1441 atagctcgga ccagacacgc ggaaaaggag acaaggagac aaggggagaa gacgattcac
     1501 tcgccgcgcc gccggtcccc atgtctcccc ctccccctgt ccccttgtct ccccagaacg
     1561 aatcggagac gaagcgtctt tcgccaccag cctgggatac actgctttaa cccatggaga
     1621 tttttggcgg catcatcgcc ctgatcgtcg gcgtcgccgg gtggtattat ctgttctact
     1681 cgcaggcggc cacgcggctc ggcgccatcg aagacgccgc cgccaaccag cgccgccatc
     1741 gcctgcggcg gaccaacggt ctcgtcatgc tcgcgctggc ggtgctgatc tacgccggcg
     1801 cccacatcga gtcgccgcgg gcattcgtca tggtgtggat cgcggtgctg ctgctgttct
     1861 tcgtattcgt cgcgcttgga tttgccgata tccgactgac ggccaagctg cggcgaaaga
     1921 gatgaaccga tgaaacgaaa ctttgcatcg ccgtcgctcg ttcacttgct gctgctcgcg
     1981 ctgctgggcg gctgcggcac cgggccggca accacgcaga gcagcctgcc tacggtgtcg
     2041 atgaagatcg gcgcgcgcac gttcacgctg gaagtcgccg ccgatgaata ctcgcgcgag
     2101 cgcgggttga tggagcgcga ttcgatgccc gctgaccacg gcatgatctt cgtcttcccc
     2161 agcgacagca gcgaaggctt ctggatgaag aacacgcaca tcccgctgga catcctgttc
     2221 atcgacgccg gtggcaaggt cgtgtcggtc cacacgatga agccgttcga catgaacacg
     2281 acgtcgagcg acggcgagta tcgctatgcg attgagctga acgccggcgc cgccgcggat
     2341 gttggagtga aggcgggcga tcacgttgat atcccgagga acgtcggcgc cgcgtcgcat
     2401 tgaccgccgc ttgcggctta gcgtctgttt cttcccgcgc gaaaacagat gctaaaccgc
     2461 aagcggctca gaaccatcac aaccgataaa gtcgcgcccc catccggtcg aagttcattg
     2521 agatgcgccg ttccgcccgc agttcacgcg gccggcgcag ttggcttttt ctcggaccac
     2581 acaagggcgc gccccatgaa cgcagcacca gccactcaac cgcacctccg cctcgttcgc
     2641 gatgatgacc cgccgcgcga cggcgacctc atcgcgccgg cgcccgcggc gtcgatcgcc
     2701 ggcgatgccg tcggtcaggt cgaagcgctg cgcacggaga tcaccgcgtt gacggcggaa
     2761 ctcagcctcc ttcgccgcaa gggcgagacg ctcaatttcc acatgtcgcg gctcgacgag
     2821 gaactgcggt tggccgcccg gctgcagcag gattttctgc ccaagtccat cccgcagatc
     2881 gggcgcgtcc acttccacac gctcttccgc cccgccggtt atgtcagcgg cgatttctac
     2941 gacgtcatgc ggctggacga aaagaacgtc ggcttctaca tcgccgacgc cgtcggtcac
     3001 ggtgttcccg cggcgttgct cacgatgttt atcaagcacg cgctggcgac caagcagatc
     3061 accaagagcg ggtatcgcct gctcggcgcg ggcgagacga tgacgcgcct gaacgaaacg
     3121 ctgatggagc aaaacctatc agccgccacg ttcgccaccg cgctgtatgg cacgatcaac
     3181 gtcgaaacgc tgcagctcac cttcgcccga ggcgggcacc cgcatccgat cctgctccgc
     3241 gctaacggcg aggtcgagcc cctgttatcc gacggcggcc tgctcggtgt cttccccggc
     3301 gaaacgttcg acgagcgcac ggtccaactc gcgcagggcg atcggctgct gctgttcacc
     3361 gatggcatcg aagtgatttt ttcggacg
//
LOCUS       DATIMH010000364         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_948440, whole genome shotgun sequence.
ACCESSION   DATIMH010000364 DATIMH010000000
VERSION     DATIMH010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948440"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VLI90_06325"
     CDS             <1..1174
                     /locus_tag="VLI90_06325"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HSV13856.1"
                     /translation="SVNNGIVEADVSGKTLSVQGVITNKGVFRASHGATMSMTAPVTF
                     TNQGAIAAEGGTVLIGGAFNCGAAGIIDIGIGGTGQGTSYGLINVTGALTLGGTINTY
                     LTGVFKPANGASFTPIKYTNKTGTFATSNFNAGGGVAFTPTFGGTSLSIKASTAASSV
                     ASVSAGKLTLNGTSAADVLSVTDSLGVVTATRGGVTSVFSTGSITGINISGGDGADTI
                     TYTGNTIPSTLAGGNGDDKITGSNASDVLSGDAGNDTEIGGAGNDRYLFAAAASAETD
                     LIGESSGGGNDTLDFSAMTTSVVIKLNSDTLATMTNRTVKTQTAGQFANIEHAIGGSG
                     NDTITGNAAANNLQGGAGNDTFFAQDGTTAKDSIDGGLGTDVVSTKDASDVVTNVP"
     gene            1616..>2651
                     /locus_tag="VLI90_06330"
     CDS             1616..>2651
                     /locus_tag="VLI90_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSV13857.1"
                     /translation="MRPARRDLLRTAGGLTALALGACRPSVALADEEAPAHGPALSPD
                     VKGVLVDLTECIGCRLCEYACKRANGFETGPLASYDDTSVFRTPRRPGTTALTVVNAW
                     PPPATGATATTSASTPPATGPARPVYTKVNCMHCNHAGCISACIVGAMRKEASGAVTY
                     DAWKCIGCRYCMVACPFGVPAYEYDNVLTPQVRKCEFCQPRTSRGELPACVKECPRQA
                     LVYGKRSDLLALARKRIGEHPDRYIDHIYGEHEVGGTSWLYLSAAPFEEAGFLTLGSA
                     APPALTEKIQHGVFKHWIAPVGWYTFLASMMWFTGRRSRLIAAKAHGSAMGYSDAAAT
                     PASPVTEEATQ"
BASE COUNT          522 a          860 c          859 g          410 t
ORIGIN      
        1 ctcggtcaac aacggcatcg tcgaagcaga cgtcagcgga aagacgctga gcgttcaagg
       61 ggtgatcacg aacaagggag tcttccgcgc atcgcacggg gcgacgatgt cgatgacggc
      121 gccggtgacg ttcaccaacc agggcgcgat cgccgccgag ggcggcacgg tgctgatcgg
      181 tggcgcgttc aactgcgggg cggccggcat catcgacatt ggcatcggcg gaaccgggca
      241 gggaacgagc tacggtttga tcaacgtcac cggcgcgctg acgctcggcg ggacgatcaa
      301 cacgtacctg accggcgtgt tcaagccggc caacggggcg tcgttcacgc cgattaagta
      361 cacgaacaag acgggcacct tcgcaacgag caacttcaac gccgggggcg gcgtggcgtt
      421 cacgccgacg ttcgggggga catctttgtc gatcaaggcc agcaccgcgg cgagcagcgt
      481 cgccagcgtc agcgccggca agctcacgct caacggcacc agcgccgccg atgtgctgag
      541 cgttaccgat tcgctcggcg tcgtcaccgc aacccgcggt ggcgtgacca gcgtcttctc
      601 aaccggcagc atcaccggca tcaacatcag cggtggtgat ggcgccgaca cgatcaccta
      661 caccggcaac acgatcccca gcacgttggc cggcggcaac ggcgacgaca agatcaccgg
      721 ttccaacgca tccgacgtcc tctccggtga cgccggcaac gacaccgaga tcggcggcgc
      781 tggcaacgac cgctacctct tcgccgccgc ggcgtcggcg gaaacagatc tgatcggcga
      841 atccagcggt ggcgggaacg acacgctgga cttcagcgcg atgacgacgt cggtcgtgat
      901 taagctcaac agcgacacgc tggcgaccat gaccaaccgc acggtgaaga cgcagacggc
      961 aggtcagttc gccaacatcg agcatgcgat cggcggcagc ggcaacgaca cgatcaccgg
     1021 caacgccgcc gcgaacaacc tgcaaggcgg agccggcaac gacacattct tcgcccagga
     1081 cggcacgact gccaaagatt cgattgacgg cggcctcggc accgacgtcg tcagcacgaa
     1141 ggatgcaagc gacgtggtga cgaacgtgcc gtgacaaaaa gcccccacgc cgggttcgga
     1201 ggtactccga cgacccggat gtttttcgcg cgcctgaaga atccgggccg ccggagtacc
     1261 tccgaacccg gcgtggggga aagcgccgcg caagttctcg aacggcgcga cacaagagcg
     1321 atgcgcctat gccacacccg ccgcacgcca caaccttccg aaatccgcaa ttgccatccc
     1381 acgccgggtt cgcagggtgc tcccgacaat ccgctttcct tgcacaaagc atccgggtcg
     1441 tcggcctgcc tgcgaacacg gcgtggcaag cgacgaaaaa ccgtcgaaaa tccagatttc
     1501 gatttgattg gaacgctggc aacgcagata ctattaatgg acttgtaagt cctttgatgt
     1561 cgatctgtgg aaatattcgc aggaactcgg catcaacacc cccaaagggc attttatgcg
     1621 ccccgcccga cgagatcttc tgcgaactgc cggtggattg accgcgctgg cgctgggcgc
     1681 ctgccggccg tccgtcgcgc tggcggatga ggaggccccg gcgcacgggc cggcgttgtc
     1741 gccggacgtc aagggcgtgc tggtcgatct gaccgagtgc atcggttgcc ggctgtgtga
     1801 gtacgcctgc aagcgggcca acggttttga gaccgggccg ctggcgtcgt atgacgacac
     1861 gtcggtcttc cgcacccccc gccggccggg gacgaccgcg ctcaccgtcg tcaacgcctg
     1921 gcctcccccc gcaaccggcg cgacggcgac caccagcgcc agcactccac ccgcgaccgg
     1981 ccccgcccgg ccggtctata ccaaagttaa ctgcatgcac tgcaatcacg ctggatgtat
     2041 ctcggcgtgc atcgtcggcg cgatgcgaaa ggaggcgagc ggagccgtca cctacgacgc
     2101 gtggaagtgc atcggttgcc ggtattgcat ggtggcgtgt ccgtttggtg tgccggcgta
     2161 cgagtacgac aacgtgctga cgccgcaggt gcgcaagtgc gagttctgcc agccgcgcac
     2221 cagccgcggg gaactgccgg cgtgcgtgaa ggagtgcccg cggcaggcgc tggtgtacgg
     2281 caagcgcagc gatctgctgg cgctggcgcg caagcgcatc ggcgagcatc cggaccgcta
     2341 tatcgatcat atttacggcg aacacgaggt cggcgggaca tcctggctat atctgtcggc
     2401 ggcgccattc gaggaggcgg gattcctcac gctgggtagc gccgccccgc cggcgctgac
     2461 ggagaagatt cagcacggcg tgttcaagca ctggattgcg ccggtcgggt ggtacacatt
     2521 tttggcatcg atgatgtggt tcaccgggcg gcgctcgcga ttgatcgccg ccaaagccca
     2581 cggctctgca atgggatatt ccgatgcagc tgcgacgcct gcgtcgcctg tcaccgaaga
     2641 ggcgacgcag g
//
LOCUS       DATIMH010000365         4769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3836256, whole genome shotgun sequence.
ACCESSION   DATIMH010000365 DATIMH010000000
VERSION     DATIMH010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836256"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VLI90_06335"
     CDS             <1..1053
                     /locus_tag="VLI90_06335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSV13858.1"
                     /translation="SPGVPGEGTRPNGPRGGSMMTGLIQSTIRGVDSTVARLYLSFFR
                     ERNALMPFLFHSLFADKNEVAENLVDPLQRTTVDEFRQFIKYYLDHGYRFVSPTDLLN
                     GLKPDGKYALITFDDGYYNNSRALPVLHEFKVPAIFFISTDHVRENRCFWWDVLYRER
                     AKQGLSHDEIYDEARSMKSMRTEDIEEKLAQTFGPTAFTPRGDVDRPFSPSDLREFAS
                     SPYVHLGNHTAHHAILTNYTPDQVRAQLLGAQQALRDITGVEPCAIAYPNGNQSDSVI
                     EICNDVGLKIGFTVRPEKTPLPLNKDRGLLRIGRFATHGDGHILEQCRTYRSDVLLYR
                     TFRDLYLRYMRGQVAQ"
     gene            complement(1216..2199)
                     /locus_tag="VLI90_06340"
     CDS             complement(1216..2199)
                     /locus_tag="VLI90_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13859.1"
                     /translation="MKRIAIAISAAMAVSGFALSAHAADPNAPVGAPAAQTDNQLDRA
                     RDNTRDATNNAGAAVNNGAAAARDKTETTGDKVRNSLDQATAGTPTANNPAPDAKDIR
                     SSIASLTNEAMTKGDLNHIVNRFVDADRNRIKNSDTYSQDYGQKLDGRIEQINETWKQ
                     KYGHTFDMKHANDVLTDQFASIQQGQIGRDAALASEVIHNSERANLSNKDNAAGNDKG
                     DENLEPGRGIAVVTVNSSHGLPDLRVPMIHEMPDQWKVNVPDSLTADKLRQNLLDHLT
                     ALGDSSSQWPSDENEAYRMAAHHVLMAVLDQPVQSHTGGTMNEGTTGGMAK"
     gene            2439..3599
                     /gene="mqnE"
                     /locus_tag="VLI90_06345"
     CDS             2439..3599
                     /gene="mqnE"
                     /locus_tag="VLI90_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HSV13860.1"
                     /translation="MTTAPATVDPALRPIAEKLAAGQILSAADGMALFQSRDLHGVGR
                     LANFVRDRLHGDNAYYNRNRHINYTNVCALSCKFCSFYRKRGEDGAYEMPVDQVVATA
                     KKAAETGATEVHIVGGLHPWLKFDYYLDMLRGIRGECPQLHIKAFTAIEIIHFSRVAR
                     LSIRDVLVALREAGLGSLPGGGAEIFDDRVHDEVFKGKVREDRWFEVHRTAHELGTYS
                     NATMLYGHVETPAERINHLIKLRELQRESLASSPANFNCCIPLSFVPENSELGFLPGP
                     TGLADLKTLAIARLMLHNIPHVKAFWIMQGIGLSQLALDWGCDDLDGTVVWYDITKRE
                     GHGTVHQQFHVDDIKRLIREAGRVPVERDTLYRPVVRDADDRAVPRPEPALV"
     gene            complement(3707..4693)
                     /locus_tag="VLI90_06350"
     CDS             complement(3707..4693)
                     /locus_tag="VLI90_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HSV13861.1"
                     /translation="MKAWLLDRIGDGVDSLRLAEIPDPTPAADEAIVSVEYAALNPAD
                     RYLAEGQYPAKPALPHILGRDGIGTIEAVGTNVTQFKPGDRAVILRSEIGVNRAGTFA
                     TKVAVPVESLVPPPPGWTDEQSAGAALVYLTAYQAITQWNDLPPASVVLITGASGGVG
                     VASTQLAAAMGHLPVALSRSDRKRATLETLGANIALDPEDAEWPAQLKSSLTGRKVDL
                     AIDNIGGPLFNDVLGTLGYHGRISVVGRLAGPVPQFNTASLFFRRIKIGGVAVGTYTA
                     VQSRAAWEKIVGILGRAGAKPQVDSTFAFDQLKEAFARLEQGPMGKVLLTLR"
BASE COUNT          853 a         1536 c         1480 g          900 t
ORIGIN      
        1 tctcccggcg taccgggaga ggggacaaga cccaacggtc cccgcggggg cagcatgatg
       61 accggcttga tccaatccac catccgcggc gtcgattcca ccgtcgcgcg cctgtacctc
      121 agcttcttcc gcgaacggaa cgcgctcatg ccgttcctgt tccactccct cttcgctgac
      181 aagaacgagg tcgcggaaaa cctggtcgat ccgctgcagc gcaccacggt ggatgagttc
      241 cggcagttca tcaagtatta cctcgaccac ggctaccgct tcgtcagccc gaccgacttg
      301 ctcaacggcc tcaagcccga cggcaagtat gcgctgatca cgttcgatga cggctattac
      361 aacaacagcc gcgcgctgcc ggtgctccac gagttcaaag tcccggccat tttcttcatc
      421 tccaccgatc acgttcgcga gaaccgctgc ttctggtggg atgtgctcta tcgcgagcgc
      481 gccaagcaag gcctgtcgca cgacgagatt tacgatgaag cgcgctcgat gaagtcgatg
      541 cggaccgaag acatcgaaga aaaactggcg cagacgttcg gaccaaccgc gttcaccccg
      601 cgcggggatg tcgatcggcc gttctcgccg agcgacctgc gcgagttcgc cagcagcccg
      661 tacgtgcacc tgggaaatca cactgcccac cacgcgatcc tcacgaacta cacgcccgac
      721 caggtgcgcg cccagctgct cggcgcgcag caagcgctcc gcgacatcac cggcgtcgag
      781 ccgtgcgcga tcgcctatcc caacggcaac cagagcgaca gcgtcatcga gatctgcaac
      841 gacgtgggcc tgaagatcgg cttcaccgtc cgccccgaaa agacccccct gccgctgaac
      901 aaagaccgcg ggctattaag gatcggccgc ttcgccaccc acggcgacgg gcacatcctg
      961 gagcagtgcc gcacgtaccg ctcggacgtc ctgctctacc gcaccttccg cgatctctat
     1021 ctacgctaca tgcgcggcca agtcgcgcag taatccgaac gggagccgct tgcggcttag
     1081 catctgtttc ccgtcgcgag agaacagacg ccaagccgca agcggctggc gcaacgcaac
     1141 ggagccgcgg atttgattcc gcggctccgt ttactgcaat cgtatcaata gcagctcaac
     1201 cgaccaccgg tgccattact tggccatacc gccggttgtg ccttcattca tcgtcccgcc
     1261 ggtgtggctc tgcaccggtt gatcgagcac cgccatcagc acgtgatggg cggccattcg
     1321 ataggcctcg ttttcatcgc tgggccattg cgacgagctg tcgcccagcg ccgtcaggtg
     1381 gtccagcagg ttctgccgca gcttgtcggc ggtcagcgaa tcgggcacgt tcaccttcca
     1441 ctggtccggc atctcgtgga tcatcggcac gcgcaggtcc ggcagcccgt gcgagctatt
     1501 cacggtcacc accgcgatcc cgcggccggg ctcaaggttc tcgtcgccct tgtcgttgcc
     1561 ggccgcattg tccttgttgg acaagttggc ccgctcggaa ttatggatca cctccgacgc
     1621 cagcgccgca tcgcggccga tctggccctg ctggatcgac gcaaactggt cggtcagcac
     1681 gtcattggcg tgcttcatgt cgaaggtgtg gccgtacttt tgtttccacg tctcgttgat
     1741 ctgctcgatg cggccgtcga gcttttggcc gtagtcctgg ctgtacgtgt cggaattctt
     1801 gatgcgattc cgatcggcat cgacgaagcg gttgacgatg tggttcaggt cgcctttggt
     1861 catcgcttca ttggtcaaag atgcgattga actgcgaatg tccttagcgt ccggggcggg
     1921 attgttcgcc gtgggcgtgc cggcggtggc ctggtccaaa ctgttgcgca ccttgtcgcc
     1981 ggtcgtctcg gtcttgtcgc gcgcggcagc ggcgccattg ttcacggccg ccccggcgtt
     2041 gttggtcgcg tcgcgcgtgt tgtcgcgggc gcgatcgagc tgattgtccg tctgcgccgc
     2101 cggcgcgccg acgggggcgt tcggatcggc agcgtgagca cttagggcaa aaccactgac
     2161 cgccatggcg gcggaaatcg cgattgcgat acgcttcata tgaggctcct ttcgaaaaaa
     2221 cttccttaaa tgggtgagac acgggtagcg cagaaatgtg ggtgcaaccg ctgcgccggc
     2281 gaccggtcga ggccggcata gaatcgacaa tcacgccgat ttcatgcagt tctcatagag
     2341 gcaggttctt gagcctgctt catcgacgat tttcaaaacg agcgaaagaa atccgcgtga
     2401 tagaagccgc cgccgcgcgt ccttatactc cgccggacat gacgaccgcc cccgcaactg
     2461 ttgatcctgc actgcgaccg atcgccgaaa aactcgccgc cggccaaatt ttgtcggcgg
     2521 ccgacggcat ggcgctattc cagagccgcg acctgcacgg cgtcggccgg ttggccaact
     2581 tcgtgcgcga ccgcctgcac ggcgacaacg cgtattacaa ccgcaaccgc cacatcaact
     2641 acaccaacgt ctgcgcgctc agctgcaagt tctgttcgtt ctatcgcaag cgcggcgaag
     2701 acggggcgta cgagatgccg gtcgatcagg tcgtcgcgac ggcgaaaaag gcggccgaga
     2761 ccggcgcgac cgaggtgcac atcgttggcg ggctgcatcc gtggttgaag ttcgactatt
     2821 acctcgacat gctccgcggc atccgcggcg agtgtccgca actgcacatc aaggcgttca
     2881 ccgcgatcga gatcatccac ttcagccgcg tcgcgcggct gagcatccgc gatgtattag
     2941 tcgcgctgcg cgaagcggga ttaggctcgc tgcccggcgg cggcgcggag atcttcgacg
     3001 accgcgttca cgatgaagtc ttcaaaggca aggtgcgcga agaccgctgg ttcgaggtgc
     3061 accgcaccgc gcacgagctg ggcacctatt ccaacgcgac gatgctctac ggccacgtcg
     3121 agacgccggc cgagcgcatc aaccatttga tcaaactgcg ggagttgcag cgcgaatcgc
     3181 tcgcgtcttc gccggcgaat ttcaattgct gcattccact gagcttcgtt ccggagaaca
     3241 gcgagcttgg atttctgcct ggacccacag ggctggctga cctcaagacg ctggcgatcg
     3301 cgcgcctcat gctgcacaac atcccgcacg tcaaagcatt ctggatcatg caaggcatcg
     3361 gcctatcgca gctggcgctg gactggggct gcgatgacct cgacggcacc gtcgtctggt
     3421 acgacatcac caagcgcgaa ggtcacggca cggtgcatca gcagtttcac gtggacgaca
     3481 tcaagcggct gatccgcgaa gccggccgcg tgccggtgga acgcgacacc ctgtatcgac
     3541 cggtggtacg cgatgcggac gatcgagcgg tgccacgccc ggagccggcg ctggtgtgat
     3601 cttctgccca caccggggtc ggagcgtact cgcgacgatc cggatctctt cgagcgccgc
     3661 agaatccggg ttgtcggagt acctccgaac ccggcgtggg gcttcatcag cgcagtgtca
     3721 aaagcacctt ccccatgggc ccctgttcca gccttgcgaa cgcttccttc agctgatcga
     3781 acgcaaacgt cgaatccacc tgcggcttcg cgccggcgcg acccaggatg ccgacgatct
     3841 tctcccacgc cgcacggctt tggaccgcgg tgtacgtgcc aacggcgacg ccaccgatct
     3901 tgatgcggcg gaagaacagc gacgcggtgt tgaactgcgg caccggcccc gcgagccggc
     3961 cgacgacgct gatgcggcca tggtaaccga gcgtgccgag cacgtcgttg aacagcggtc
     4021 cgccgatatt gtcgatcgcg agatcgacct tgcggccggt cagtgatgac ttcaattgcg
     4081 ccggccattc agcgtcttct gggtcgagcg cgatgttcgc gccaagtgtt tcgagcgtcg
     4141 cgcgtttcct gtcgctgcgc gacagtgcga ctggcagatg ccccatcgcc gcggcgagtt
     4201 gtgtgctggc gacgccgacc ccaccgctgg cgccggtgat cagcacaacc gatgcgggcg
     4261 gcaggtcgtt ccactgcgtg atcgcctgat atgcggtgag atagacgagc gctgcgccgg
     4321 cggattgttc atcggtccaa cctggtgggg gcggcacgag cgactcgacg ggaaccgcga
     4381 ccttggtcgc gaacgtgccg gcgcggttga cgccgatctc gctgcgcagg atgacggcgc
     4441 gatcgccggg cttgaactgc gtgacgttgg tgccgacggc ttcgatcgtg ccgatgccat
     4501 cgcggccgag gatgtgcggg agcgcgggct tggctgggta ctgtccctcg gcgagataac
     4561 ggtcggcggg gttgagcgcg gcatattcga cggagacaat cgcttcatcc gcagcggggg
     4621 ttggatcggg gatttcggca agtcggagtg aatcgacacc gtcgccgatg cggtcgagca
     4681 gccaggcttt catttgttac acctcgtcgt tgcgggactg gcgaagtgta gtcttccggc
     4741 gcggacctgc gtagcggaag cccggcagg
//
LOCUS       DATIMH010000366         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5283790, whole genome shotgun sequence.
ACCESSION   DATIMH010000366 DATIMH010000000
VERSION     DATIMH010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5283790"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..388)
                     /locus_tag="VLI90_06355"
     CDS             complement(59..388)
                     /locus_tag="VLI90_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13862.1"
                     /translation="MSKPAGLTFEPVNEHGILTVHADDEFFPFSATDKAKLVAQIDAA
                     MKVVQFDFSPVQVAGKSVAFTRADQARVIRQAALHWLGGARVMSSEEMRRTKCESPNQ
                     IRMTNDE"
     gene            complement(385..1089)
                     /locus_tag="VLI90_06360"
     CDS             complement(385..1089)
                     /locus_tag="VLI90_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HSV13863.1"
                     /translation="MSKSSLRRRGFIMLFGTRNIVSGSSNDEQPIDTRCPRCGAASAM
                     IGKTVRPWFTLFLLPLFPVGAARTFTQCTNCQASFAVAPQQLANQAARVDAKQNERAI
                     AMYNSLRASPGNSITLNDVMQLYASLGEYDQAISAAADFPQALNNSEQCMATLGRVYL
                     AKNEHAAAIKWFEAAIARNPSQGEAQYYKAAAYLTATPPEPQKAVPAARAARNAGYPN
                     ADELLREAEEKVRGAA"
     gene            complement(1257..2387)
                     /locus_tag="VLI90_06365"
     CDS             complement(1257..2387)
                     /locus_tag="VLI90_06365"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV13864.1"
                     /translation="MPARYLVLSASVGAGHMRAAQAVELALRELAPDATVQNIDLLTL
                     TNAAFRRVYGSAYLDLVNKAPHVLGYIYDLLDRPREPDSARDKLRLAIEKLNLAKFRD
                     MLDCDHWDVVVNTHFLPAEIIAHLRRDGKCSLKQMTVTTDFETHRLWVNQPCDHYTTA
                     TAEGAAYLHHWGVPKEDISVTGIPIHPAFAKPRDRQACLKSQGLSGDRPIVLQSAGGF
                     GVGPIGKLYDALLAIEHPLEVVVVAGKNAAVKEELAKVTVPPRHRAHLLGFTDKMHEL
                     MAVADIVLSKPGGLTTSEVLASGAAMAIVNPIPGQESRNSDYLLENGAAIKINNLPTL
                     SLKLNELLANPARLESLKANARRIAKPQAAYDVARRAIAMHS"
BASE COUNT          438 a          798 c          814 g          484 t
ORIGIN      
        1 tgacaaaatc cgaatgacga aaacgcgcga tccgcgcgcg agagcgcgcg tgtgtccgtc
       61 attcgtcatt cgtcattcgg atttgattcg gggattcgca cttcgtcctt cgcatttcct
      121 cactcgacat caccctcgcc ccaccgagcc agtgcagcgc cgcttggcgg atgactcgcg
      181 cttgatcggc ccgcgtgaac gccacggatt ttccggcgac ttggacgggg ctgaagtcga
      241 actgcacgac cttcatcgcc gcgtcgatct gcgcgaccag cttcgctttg tccgtggccg
      301 agaacggaaa gaactcgtcg tccgcgtgga ccgtgaggat gccgtgctcg ttcacgggct
      361 cgaaggtgag tccggcgggt ttgctcacgc agcgcctcgc actttctctt ccgcttcgcg
      421 cagcaactca tccgcattgg gataaccggc gttgcgcgcg gctcgcgcgg cgggaaccgc
      481 cttctgcggt tccggtgggg tcgccgtcag atacgcggcg gccttgtagt actgcgcttc
      541 gccttgcgat ggattgcgcg cgatcgccgc ttcgaaccat ttgatcgccg cggcgtgctc
      601 gttcttcgcc aggtacaccc ggcccagcgt tgccatgcac tgctcgctgt tgttcagcgc
      661 ctgcggaaaa tccgccgcgg cggagatggc ctggtcgtat tcgcccaacg atgcgtacag
      721 ctgcatcacg tcgttcagcg tgatcgagtt gccgggtgaa gcgcgcaggc tgttgtacat
      781 cgcgatcgcg cgctcgttct gcttcgcatc cacgcgagcc gcctggttgg ccaattgctg
      841 cggcgctacg gcgaatgacg cctggcaatt ggtgcactgc gtgaacgttc gcgcggcgcc
      901 aaccgggaac agcggcagca aaaacagcgt gaaccacggc cgcacggtct tgccgatcat
      961 cgccgacgcc gccccgcacc gcgggcagcg cgtgtcgatc ggctgctcgt cgttcgatga
     1021 gccggagacg atgttccgag ttccgaacag catgatgaac ccgcggcggc ggagtgagga
     1081 cttcgacacg cgcgcaagct taacgccggg cggggcgaaa acgaaaagaa acgcgcgggc
     1141 gctgtattca cacgccgggt tcggaggtac tccgacgacc cggatgcttt gcttcactcg
     1201 cgccgaagaa tccgggtcgt cgcgagtacg ctccgaaccc ggcgtggggg gggatctcaa
     1261 gaatgcattg cgatcgcgcg gcgcgcgacg tcgtacgccg cttgcggctt cgcaatccgc
     1321 ctcgcattcg ccttcagtga ctccagtcgc gccgggttcg ccagcagctc gttcagcttc
     1381 agcgacagcg tcggcaggtt gttgatcttg atggcggccc cgttctccag caggtaatcg
     1441 ctgttgcggc tctcctgccc ggggatcggg ttcacgatcg ccatcgcggc cccgctggcc
     1501 agcacttccg acgtcgtcag cccgccgggc ttggagagga cgatgtcggc gacggccatc
     1561 agctcgtgca tcttgtcggt gaaccccagc aagtgcgccc gatgccgcgg cggcacggtg
     1621 accttcgcca gttcctcttt gaccgccgcg tttttgccag cgacgacgac gacctccagc
     1681 gggtgctcga tcgccagcag cgcatcgtac aatttgccga tcggcccgac cccgaacccg
     1741 ccagccgatt gcaggacgat cggccggtcg ccgctcaggc cttgcgattt caaacacgcc
     1801 tggcgatcgc gcggcttggc gaaggcgggg tgaatcggga tgccggtcac gctgatgtct
     1861 tctttcggca cgccccagtg atgcaggtac gccgcccctt cggcggtcgc ggtcgtgtaa
     1921 tgatcgcacg gctgattgac ccacagccgg tgcgtttcga aatcggtggt gacggtcatc
     1981 tgcttcagcg agcatttccc gtcccgccgc aggtgcgcga tgatctcggc gggcaggaaa
     2041 tgggtgttga cgacgacgtc ccaatgatcg caatcgagca tgtcgcggaa tttcgcgagg
     2101 ttcagcttct cgatcgccag ccgcagcttg tcgcgcgccg aatccggttc tctcggccgg
     2161 tcgagcaaat cataaatgta ccccagcacg tgcggggcct tgttgaccag gtcgaggtaa
     2221 gccgagccat acacgcggcg aaacgcggcg ttggtgaggg tgagcaggtc gatattttga
     2281 acggtggcgt cgggggcgag ttcgcgcagc gccagttcaa cggcttgagc ggcgcgcata
     2341 tgcccggcac cgacggacgc ggagaggacg aggtagcgag cgggcatcta tgaattgaaa
     2401 acgaaatgac gtggaatcgc aaaaagacgc gactcccacg tgcgtagcgg tagccccgaa
     2461 ggggcacgtg tttttcgtct tcgcgaagcc tcgaaaagag attgaaccac gaagctcacg
     2521 aagggcacga agaa
//
LOCUS       DATIMH010000367         9012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3550163, whole genome shotgun sequence.
ACCESSION   DATIMH010000367 DATIMH010000000
VERSION     DATIMH010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3550163"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..4644
                     /locus_tag="VLI90_06370"
     CDS             112..4644
                     /locus_tag="VLI90_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSV13865.1"
                     /translation="MTRSNRSPMPLIELLELRVLFAVHNDSFDVTDLTQLRSDPNFSA
                     IDGSGVGIAVLDTGVYDQNPDLNSNVVGWYNAVTTQPNATLTSANDPSNAFDHQGHGS
                     HTSGIAASSNPAIGVAFHAHLIDIRVFADQGEAQLGGDPILRGLEWVALHANDPQYNI
                     KVVNMSLGFGGVNANTVTSQYAQQDETREINTLQSMGITVVTSSGNSYANDPVPGASF
                     PAIVSTISVANTYADNGQPSDFGIPYGGSGDQFYAVEYHAQPDLIAATSQRSSLPNQV
                     AAPGQDIYSTWNGTASADNSSDPNHKLDSGTSMAAPFVAGTVALMQQAAKMFGGQYFS
                     NPQEILQILKDTADTITDFNVADNGRVAASGNTLLPLPETGLQFQRINVLHAIERVKQ
                     LVQQGNPTPNPNNGADTDSVTANATSLGNLDGTQVLGAGGNIGADGSVNVGANDIDLY
                     QMNVTSIGNFTIQLSLPNGGTAFGAALRLFDSTGVEIAHSTATGGVYPSLSTDPNSPL
                     PVGVYYVGVSATNNLSYDVNTGAGATGGTTVGDYTLSIGLQNPDPNGVAAGAVDANLT
                     LPDVIGPATNTPALKFTGTLGSDPGPVAGGPRISVPNGDVDMFRVIAPDNGDLVVDTE
                     ALGVYGADAADTYVRVFDSNLNQIAFNDDKQPGVNTDSFVSAPVSIGQTYYVAVTNFA
                     NADFDPQNPYNRAAGSTTPEQSYDLYLSFDNGDINGTVFGAVAESVGNAVQGTVGADQ
                     GVPLIGANGGFKDVDFLQYTPTASGLLDFAAGSASTGFQPSIALWQFGSDNSSIVQVA
                     QTTSTNPALIYPVTANQELFLSVTGSGNQNFNWFAPASGTGGQTGNYTLNTSVLPSSD
                     MSTLSDNAINDTPTNIALGQSIVANLSMDNALVVGDTDVDIYKFVAPTSLHVTIRTNT
                     SQEGSADTVLRVFDASGNPLASNDNADPNGTASAIALDVTAGTTYYIGVSGAGANAMS
                     YDPVTGANAGSGSTGNYSIAIFAPAFVAAASPVSEPLPGTTATETFTVTLAEPQSASA
                     TVDYATADGTAVAGADYDAVSGTLTFAPGETTKMIPVTVHGDLLSNADKTFTLNLTNP
                     SAGTTVSTPSVTATITNVAVTETDFGGNKSATYTDSTGHRVLMRLVGPGNGQIFFAGG
                     SADPAQVNVTNTTGASTLVVAASNGATTLPGLSVDGSLAALVATTANITGNVHITGTA
                     GRIFLGNVSGGSITIDGASAAGSTIRLGAVVDSSLTTASPIAALILTSWTDTGGSADT
                     ITAPSIAVLRSLGAFNAGLNVSGGDLRIAQFLGGITGGTWAVSGSAATLVAKNTAAGW
                     SATFGGSVGVFVSQGNASGSLTAQSIRLMRVAGNLAQANITLSGGGTSLGTLVVVGVD
                     SASHVTTAGNISVVSVGAMTDGSVEAGVDPAVTGLPISTTAFTTPASIGVFAVTGAAP
                     SPLAFADSIIAAEDLGKVIVRRVLFDNNATPFGFASRSLALFADFENGKAPVVWTPKK
                     PASDLTFAGDFKVSLL"
     gene            complement(5084..7135)
                     /locus_tag="VLI90_06375"
     CDS             complement(5084..7135)
                     /locus_tag="VLI90_06375"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HSV13866.1"
                     /translation="MRSIIQAVFVLATMSLVAHAEPPQLTVHADQPGPRISPMLYGLM
                     TEEINHSYDGGLYAELIQNRIFRDAPIPAAPHRGATISLTTQPVPPPEQPYHWSLVTD
                     GAAAGSMIVDTDNPVNTTALTHSLRLDVKSTTGGRVGVANDGYWGIPVKPDTTYRASF
                     YARASQGLAGPLTVSIESNDGQTVAASAQVPSVTTEWKRYELTLKTGNVAASTANRFV
                     ISTSSPGSIWFNLISLFPPTYHDRPNGNRVDLMELLSAMHPAFLRFPGGNYLEGDYFN
                     ERFNWKETIGPLEQRPGHNSPWRYRSTDGLGLLEFLEWCQDLKMQPVLAVFAGYTLRG
                     QHIAGGPELQPYVDEALEEIEYVTGDAGTRWGQERAKDGHPAPFDLTYVEIGNEDNFD
                     ARSGHDTYDSRFTQFYDAIKAKYPKLQLIATAKVHTRTPDVLDDHYYRPGSVMARDAH
                     HYDHTNRNGPKIFVGEWASREVPPNRTLTPSLHSALGDAAWMTGLERNSDIVVISCYA
                     PLLTNVNRGAWQWLTDLIGYDAGRSFGSPSYYVQAMFAQNRGDVVLPVTLTDATDPNA
                     VGVMNSPTAPTTMPASAEGKQIESIFATASRDEASGDVIVKFVNMRGTPQPVRVKLDG
                     VTSVNAAAGTVVSGQPKDMNSVQEPTKVAPRPLEVPASGKDFTQEFPAYSATVLRFKT
                     R"
     gene            complement(7174..7944)
                     /locus_tag="VLI90_06380"
     CDS             complement(7174..7944)
                     /locus_tag="VLI90_06380"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV13867.1"
                     /translation="MLDSTTRFSDRVAYYIRSRPRYPAALLRFFQDELGLLPSHAVAD
                     IGAGTGLLTELFVRNGNVTYAVEPNQEMRAAADAALSKCANYRSTRGTAEATTLADAS
                     MNFATAGQAFHWFEPVATRREFARILEPGGFVALVWNERPEHASTFAAAYEQLVSEYH
                     ADPTAMSTRRQLLATDAAIAAFFAPSSFDARRFDNPQSLDRSQLIDRLSSASYTPLPP
                     DPRHAQMLAAANALFDRHQVDEYVVMPHDTRVFFGRLH"
     gene            complement(7953..>9012)
                     /locus_tag="VLI90_06385"
     CDS             complement(7953..>9012)
                     /locus_tag="VLI90_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13868.1"
                     /translation="FVDAHSYWDHPRFPHKAWDASDWLINNTPMVDQPERATLWSLIG
                     TRVAGKPFTVTEYNHPAPNEWQAECVPIIATCAALQDWDGVFLFAYSHSADFEKEKVD
                     GFFNIEGNPLKMPLMPMGARLFLGGAIEPRGSAVGVAMPHELMVQTSPSLSTDQGAFL
                     RSVLGLKWQHLLSKRISVHFDDTANDISGHAGGPEVGWTSDGAGTGTGRYTCFGPKAA
                     VFTGFAAGRMPIDLGAVRIESLQMPFATIIVTPADPTTTIEQADRLLVMAVARGQNTD
                     MKWDAQRHTVSNEWGSAPPRVEVVRGSISVSGQWSHVRPLSSSGQPVDRDATTQLAGG
                     RTTIRLGETPALAYEVSR"
BASE COUNT         1598 a         3044 c         2816 g         1554 t
ORIGIN      
        1 cgtgtcttta taacaatgcg cctccgccct tgtcgcgcgc cgcgatgcag gcacccacgc
       61 acgcacgaat cgtaatagtg ttacccaccg cttgagtgag gagaaaccac cgtgacccgt
      121 tccaatcgtt cgccgatgcc cctgatcgaa ttgctcgaac tccgcgtgct cttcgcggtt
      181 cacaacgact cgttcgacgt gaccgacctg acacagctgc gcagtgaccc gaacttctcc
      241 gccatcgacg gcagcggcgt cggcattgcc gtgctcgaca ccggcgtgta cgaccagaac
      301 ccggacctga acagcaacgt cgtcggctgg tacaacgcgg tgacgacgca gccaaacgcc
      361 acgctgacct cggcgaacga tccgtcgaat gctttcgatc accagggcca cggcagccac
      421 acgtcaggca tcgcggcgtc gtcgaacccg gccatcggtg ttgcctttca cgcgcacctg
      481 atcgacatcc gcgtcttcgc cgaccagggc gaagcgcagc tgggcggcga tccgatcctg
      541 cgcgggctgg aatgggttgc cctgcacgct aacgatccgc agtacaacat caaggttgtc
      601 aacatgtcac tgggctttgg cggtgtgaat gccaacacgg tcacctcgca atatgcccag
      661 caggacgaga cgcgcgagat caacacgctg caatcgatgg gcatcaccgt cgtcacgtcg
      721 tccggcaaca gctatgcgaa cgatccagtg cccggggcgt cgttcccggc gatcgtctcg
      781 acgatcagcg tcgccaacac gtacgccgac aacggccagc cgtccgactt cggcattccc
      841 tacggtgggt ccggcgatca gttttatgcg gtggagtatc acgcgcagcc ggatctgatc
      901 gcggcgacaa gccagcgcag ttcattgccg aaccaagtcg ccgcgccggg gcaggacatc
      961 tacagcacgt ggaacggcac ggccagcgcc gacaacagca gcgacccgaa tcacaagctc
     1021 gacagcggca ccagcatggc ggcgccgttc gtcgcgggca cggtcgcgtt gatgcagcag
     1081 gcggcgaaga tgttcggcgg gcaatatttc agcaacccgc aggagatcct gcagatcctg
     1141 aaggatacgg ccgacacgat caccgacttc aacgtcgccg acaacggccg ggtggccgcc
     1201 agtggtaaca cgctgctgcc gctgccggag acgggactgc aattccagcg catcaacgtg
     1261 ctgcacgcca tcgagcgcgt gaagcaactg gtgcagcagg ggaatcccac gccgaacccc
     1321 aacaacggcg cggacaccga ttcagtcacc gccaacgcca cgtcgctggg caacctcgac
     1381 ggcacgcagg tgctgggcgc cggcggcaac atcggcgccg atggatcggt caacgtcggc
     1441 gccaacgaca tcgacctgta ccagatgaac gtcacgtcga tcggcaactt cacgatccaa
     1501 ctgtccttgc cgaatggcgg gaccgcgttc ggcgcggcgc tgcggctgtt cgacagcacc
     1561 ggcgtcgaaa tcgcccactc caccgccacc ggcggggtgt atcccagcct cagcaccgat
     1621 ccaaacagcc cgctgccggt cggcgtctat tacgtcggcg tcagcgcgac caacaacctg
     1681 tcctacgacg tcaacaccgg cgccggcgcg accggtggga cgaccgtcgg tgattacacg
     1741 ttgtcgatcg gcctgcagaa ccccgatccc aacggcgtgg ccgccggcgc ggtcgacgcc
     1801 aacctcacgc tcccggacgt gatcggcccg gccaccaata cgccggcgct gaagttcacc
     1861 ggcacgctcg gttccgatcc cggtccggtg gccggtgggc cccgcatctc ggtgcccaac
     1921 ggcgacgtcg atatgttccg cgtcatcgcc cccgacaacg gcgatctcgt cgtcgatacc
     1981 gaagcgctcg gcgtctacgg cgcagacgcg gccgacacgt acgtgcgcgt cttcgattcc
     2041 aacctcaacc agatcgcgtt caacgacgac aagcagcccg gcgtgaacac cgacagcttc
     2101 gtcagcgcgc cggtttcgat cggacagacc tactatgtcg cggtcaccaa ttttgccaac
     2161 gccgatttcg atccgcagaa tccttacaac cgcgctgccg gcagcaccac gccggagcag
     2221 agctatgacc tgtacctctc gttcgacaac ggcgacatca acggcaccgt cttcggcgcg
     2281 gtggccgagt cggtcggcaa cgccgtgcag ggcacggttg gcgcggacca gggcgtgccg
     2341 ctgatcggcg ccaacggcgg gtttaaggac gtggacttcc tgcagtacac gcctactgcg
     2401 tcgggcctgc tcgatttcgc cgccgggtct gcctcgaccg gttttcagcc gtccattgcg
     2461 ctctggcagt tcggcagcga caacagctcg atcgtgcagg tggcccagac gacgtcgacc
     2521 aaccccgcgc tgatttaccc ggtgacggcc aaccaggaat tgttcctgtc ggtgaccggc
     2581 tcggggaacc agaacttcaa ctggttcgcc cccgccagcg gcaccggcgg gcagacgggc
     2641 aattacacgc tgaacaccag cgtgctgccg agcagcgaca tgagcacgct cagcgacaac
     2701 gcgatcaacg acacgccgac gaatatcgcg ctcggccaga gcatcgtcgc gaacctgagc
     2761 atggacaatg cactggtcgt cggcgacacc gacgtggaca tctacaagtt cgtcgcgccg
     2821 accagtctgc acgtgacgat ccgcacgaac accagccagg aaggcagcgc ggacacggtg
     2881 ctgcgcgtct tcgatgccag cggaaacccg ctggcgagca acgacaacgc cgatcccaac
     2941 ggcaccgcca gcgccatcgc gctcgacgtg acggcgggga cgacgtacta catcggcgtc
     3001 agcggcgccg gcgcgaatgc gatgtcgtac gatccagtca ccggcgccaa cgccgggtcc
     3061 ggcagcacgg gcaattattc gatcgccatc ttcgcccccg cgttcgtcgc ggcagcgtcg
     3121 ccggtcagcg aaccgttgcc agggacgacc gcgaccgaga cgttcaccgt cacgctggcc
     3181 gaaccgcagt cggccagcgc gaccgtcgat tacgccaccg ccgacggcac cgccgtcgcc
     3241 ggcgccgatt acgacgccgt cagcggcacg ctcaccttcg ccccgggcga gacgaccaag
     3301 atgatccccg tcaccgtgca cggcgatttg ctcagcaatg ccgacaagac cttcacgctg
     3361 aacctgacca accccagcgc gggcaccacc gtgtccacac cgtcggtcac cgcgacgatc
     3421 accaacgtcg ccgttaccga aaccgacttc ggcggcaaca agagcgcgac ctacaccgac
     3481 tcgaccggtc atcgcgtgtt gatgcgattg gtggggcccg gcaacgggca gatcttcttc
     3541 gccggcggat cagccgatcc ggcgcaggtc aacgtcacca ataccaccgg cgcgtctacg
     3601 ctggtggtcg cggcttcgaa cggcgcgacc acgctgccgg gcctgagtgt ggacggctcg
     3661 ctcgccgcgc tggtcgcgac gaccgccaac atcaccggca acgtgcacat caccggcacc
     3721 gccggtcgca ttttcctcgg gaacgtcagc ggcgggagca tcaccatcga tggcgccagc
     3781 gccgccggca gtaccatccg cctcggcgcg gtggtcgatt cgtcgctgac cacagccagc
     3841 ccgatcgcgg cgctcatcct caccagctgg accgacacgg gtggcagcgc cgacacgatc
     3901 actgccccct cgatcgcggt gctgcgatcg ctcggcgcgt tcaacgcggg cttgaacgtc
     3961 agcggcggcg acctgcgcat cgcgcaattc ctcggcggca tcaccggtgg cacgtgggcc
     4021 gtcagcggct cggccgccac cctcgtcgcc aagaacaccg ccgccggttg gagcgccacg
     4081 ttcggcggca gcgtcggtgt gttcgtgtcg caaggcaacg cgtccggctc gctcaccgcg
     4141 cagtcgatac ggctcatgcg cgtggccggg aatctcgctc aagcgaacat cacgctcagc
     4201 ggcggcggca cgtccctcgg cacgctggtc gtcgttggtg tcgatagcgc atcgcacgtg
     4261 accaccgcgg gcaacatcag cgtcgtcagc gtcggcgcca tgaccgacgg ctccgtcgaa
     4321 gccggtgtcg atccagccgt gacaggatta ccgatcagca cgacggcctt cacgacgccg
     4381 gccagcatcg gcgtcttcgc cgtcaccggc gcggccccgt cgccgctggc cttcgccgac
     4441 tcgatcatcg ccgccgagga cttgggaaaa gtaatcgtcc gccgcgtcct gttcgacaac
     4501 aacgccacgc cgttcggctt tgcgagcagg tcgctggcgc tgttcgcgga tttcgaaaac
     4561 ggcaaagcgc cggtggtgtg gacgccgaag aagcccgcga gcgatctaac gttcgcgggg
     4621 gactttaaag tgtcgctgct gtaacgtgat ttcgcatcga tgtcctgagc tcgagcgttt
     4681 gtgatctctt cgtagcggtc atcgcgtgcg aagcacgtac cggtgaacca gtccaagatc
     4741 tagccgagaa gttagtgaac catccgtcgc gcatgacgcg tagcagtcgc ttgcgtcatc
     4801 gcgggtccgc cgcgtctccg cgacgacgca agcgacagct atgttccccc gggaaaatca
     4861 atccttcaca tacttcccgg ctgaatcctt cggctgcggc gaggcgaaac tcagaatcga
     4921 acaagcgatg gcgcgtgcgg ggcaattcta gtatcgcgcg ccggatgatt tcgttatcgc
     4981 atgccccatg atagcccagg ccggaaggcc gggggtcgca cttgcgaaag cgctgattcg
     5041 ctgatcgatc cccggacttc cggcctgggc tattaatggt tctctatcgc gtcttgaacc
     5101 gcagcaccgt cgcgctgtac gccgggaact cctgcgtgaa atcttttccg ctcgctggga
     5161 cttccagcgg gcgcggcgcg accttcgtcg gctcctgcac gctgttcatg tccttcggct
     5221 gcccgctgac gacggttccc gctgcggcgt tcacgcttgt cacgccgtcc agcttcacgc
     5281 gcaccggctg cggcgtgccg cgcatgttca cgaactttac gatcacatcc ccgctcgcct
     5341 catcccggct ggcggtggcg aagatcgatt cgatctgctt cccttcggca gacgccggca
     5401 tcgtcgtcgg cgccgtcggt gaattcatca cgcccaccgc gttcgggtcc gttgcatccg
     5461 tcagcgtcac cggcagcacg acgtcgccgc ggttttgggc gaacatcgcc tgcacgtagt
     5521 acgacggcga accgaagctg cgccccgcgt cgtagccgat caggtccgtc aaccattgcc
     5581 acgccccgcg gttcacgttg gtcagcagcg gcgcgtagca ggagatgacg acgatgtcgc
     5641 tgttgcgctc cagccccgtc atccacgccg cgtcgccgag ggccgagtgc aggcttggcg
     5701 tgagcgtgcg gttcggcggc acctcgcgcg acgcccattc gccgacgaag atcttcggcc
     5761 cgttccggtt cgtgtggtcg taatgatgcg catcgcgcgc catgacagac ccggggcgat
     5821 agtagtgatc gtccagcacg tccggcgtgc gcgtgtgcac cttcgccgtc gcgatcaatt
     5881 gcagcttcgg atactttgcc ttgatcgcat cgtaaaactg cgtgaaccgg ctgtcgtatg
     5941 tgtcgtgacc cgagcgcgca tcgaaattgt cctcgttgcc gatctcgacg taggtcagat
     6001 cgaacggcgc gggatggccg tccttggcgc gctcctgtcc ccagcgcgtg ccggcgtcgc
     6061 cggtgacgta ctcgatttct tccagcgctt cgtccacgta cggctgaagc tccggcccgc
     6121 cggcgatgtg ctggccgcgc aatgtgtaac cggcgaacac ggccagcacc ggctgcatct
     6181 tcaggtcttg gcaccactcg agaaattcga gcaagcccag tccatcggtc gatcgatatc
     6241 gccacgggct gttgtgcccg gggcgctgct cgagcgggcc gatcgtttcc ttccagttaa
     6301 aacgctcgtt gaagtaatcg ccttccagat agttcccgcc ggggaaacgc aggaacgccg
     6361 ggtgcatggc ggacagtagc tccatcagat cgacgcggtt gccgttgggc cgatcgtgat
     6421 atgtcggcgg gaagagcgag atgaggttga accagatcga tcccggcgag ctggtcgaga
     6481 tgacaaagcg attggcggtg gacgccgcga cgttgcctgt cttcagcgtc agctcatatc
     6541 gcttccactc tgtggtgacg ctcggcacct gcgccgatgc ggcgacggtc tgcccgtcgt
     6601 tgctttcgat gctgaccgtg agcggtcccg ccaaaccctg tgaagcgcgg gcgtaaaacg
     6661 acgcgcgata ggtcgtgtcc ggcttcaccg gaattcccca gtacccgtcg ttggcgacgc
     6721 cgactcggcc gccggtggtg gacttcacgt cgagccgcag gctgtgcgtt aatgccgtgg
     6781 tgttcacggg attgtccgtg tccacgatca tcgaccccgc cgcggcgccg tcggtgacga
     6841 gcgaccagtg atacggctgc tccggcggcg gcaccggttg agtcgtcagc gagatcgtcg
     6901 cgccgcggtg cggcgccgcc ggaatcggcg cgtcgcgaaa gatgcggttc tggatcaact
     6961 ccgcgtaaag cccaccgtcg tacgagtggt tgatctcttc ggtcatcagg ccgtacagca
     7021 tgggactgat gcgcggccct ggttggtcgg catgaaccgt cagctgcggc ggttcggcgt
     7081 gcgcgacgag cgacatcgtc gcgagaacga atacggcctg aatgatcgag cgcaatgcgg
     7141 tctccttgta atgacgcggc agtatagcga gcgttaatga agtcgcccaa agaagacgcg
     7201 cgtgtcgtgc ggcatcacga cgtattcatc aacctgatgc cgatcgaaca gcgcgttcgc
     7261 cgcggccagc atctgtgcat gccgcgggtc gggcggcaac ggcgtgtatg aagccgagga
     7321 caggcggtcg atcaactggg agcgatccag gctctgcggg ttgtcgaatc ggcgcgcgtc
     7381 gaatgaactg ggcgcgaaga acgcggcgat ggccgcgtcg gtcgcgagca gttgtcggcg
     7441 ggtagacatg gcggtcggat cggcgtggta ttcgctgacc agctgttcgt acgcggccgc
     7501 gaaggttgat gcatgctctg gtcgctcgtt ccagacgagc gcgacgaaac cgccgggctc
     7561 gaggatgcga gcgaactccc tccgtgtcgc gacgggttcg aaccaatgaa acgcctgccc
     7621 cgcggtcgcg aagttcatgc tggcatccgc gagcgtcgtt gcctccgcgg tgccacgcgt
     7681 gctgcgatag ttcgcgcatt tgctcagcgc ggcgtcggct gccgcgcgca tttcttggtt
     7741 gggttccacg gcgtaagtca cgttgccgtt gcggacgaac agttcggtaa gcaggcccgt
     7801 gccggcgccg atgtcggcaa ccgcatgtga tggcaacagt cccagctcgt cctgaaagaa
     7861 acgcagcagc gctgcgggat aacgagggcg cgagcggatg tagtacgcga cgcgatcgga
     7921 aaagcgcgtg gtggagtcga gcatgatgcg ccttaccgcg agacttcgta cgcgagcgcc
     7981 ggcgtctcgc cgagtcggat cgttgtgcga ccgccggcaa gttgggtcgt cgcgtcgcga
     8041 tccaccggct gcccgctcga cgacagcggt cgcacgtgcg accattggcc gctcacgctg
     8101 atcgacccgc gcacgacttc gacccgcggc ggggccgagc cccattcgtt cgacaccgtg
     8161 tgccgctgcg cgtcccattt catgtccgtg ttctggccgc gggccaccgc catcaccagc
     8221 aaacgatccg cctgctcgat cgtcgtcgtc ggatcggccg gcgtcacgat gatggtcgcg
     8281 aacggcatct gcagcgattc gatccgcacc gcccccaggt caatcggcat cctccccgcg
     8341 gcgaaacccg tgaagacggc ggccttgggg ccaaagcagg tgtaccgccc ggtgccggtg
     8401 ccggcgccgt cgctggtcca gccaacctca ggcccacccg cgtggccgct gatgtcgttg
     8461 gcggtgtcgt cgaaatgaac gctgatgcgc ttgctcagca ggtgttgcca cttcaggccg
     8521 aggaccgatc gcagaaatgc gccttgatcg gtgctcaggc ttggcgaggt ctgcaccatc
     8581 agctcgtgcg gcatcgccac accgaccgcg ctgccgcgcg gttcgatcgc gccgccgagg
     8641 aacagccgcg cgcccatcgg catcagcggc atcttcagcg ggttgccttc gatgttgaag
     8701 aagccatcca ccttttcctt ctcgaagtcg gcgctgtggc tgtaggcgaa cagaaagaca
     8761 ccgtcccaat cctgcagcgc ggcgcacgtc gcgatgatcg gcacgcactc cgcctgccat
     8821 tcattcgggg cagggtggtt gtactccgtc accgtgaacg gcttgcccgc cacgcgcgtg
     8881 ccgatcaggc tccacagcgt cgcgcgctcc ggctggtcca ccatcggcgt gttgttgatc
     8941 aaccagtccg atgcatccca cgccttgtgc ggaaagcgcg ggtggtccca gtatgaatgg
     9001 gcatcgacga aa
//
LOCUS       DATIMH010000368         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_665278, whole genome shotgun sequence.
ACCESSION   DATIMH010000368 DATIMH010000000
VERSION     DATIMH010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665278"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VLI90_06390"
     CDS             complement(<1..523)
                     /locus_tag="VLI90_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13869.1"
                     /translation="MPREERMFGDIVDPSIKMGNKQGASVAMTILLEAAIIAAIVIIP
                     LMAYDVIPTPADSMSAFVAAAPPPPPPPPPPPPPAAQAPKPVEVVNPNAAPTEAPAEI
                     KPETNIDTNFDRSVGVEGGVPGGVVGGVVGGLPEAPPPPPPPPQAPVRVGGNIKPPQQ
                     LKRVNPTYPAIAQS"
     gene            complement(842..>2919)
                     /locus_tag="VLI90_06395"
     CDS             complement(842..>2919)
                     /locus_tag="VLI90_06395"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HSV13870.1"
                     /translation="NPIPGKAVATTLPIILGFSVIGILGSYSVAWFGIRVNTYANSRT
                     AFASLEGKPYAVYAIPLRAGMSIGMLLISVELLIMLLILLFIPGDYAGPCFIGFAIGE
                     SLGAAALRIAGGIFTKIADIGSDLMKIVFNIKEDDARNPGVIADCTGDNAGDSVGPSA
                     DGFETYGVTGVALITFIMLAVKDPAVQGKLLVWIFSMRIMMVIASAGSYFINEAIAKA
                     RYGNATKMNFEAPLTSLVWLTSIVSVVLTYIVSNLLIADLGSDGTLWWKLASIITCGT
                     LAGAIIPELVKVFTSVESSHVKEVVTASREGGASLNILAGFVAGNFSAYWLGLTIVAL
                     MSIAYFVSASGAAMSDLMLAPAVFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVFE
                     LSVIEQVPNIHQELKRDFGLTVQFDKAKDLLEENDGAGNTFKATAKPVLIGTAVVGAT
                     TMIFSIIVALTEGLKPELAVNLSLLHAPFLLGLVTGGAIIYWFTGASTQAVTTGAYRA
                     VEFIKANIKLDDTVTKASVEDSKKVVEICTQYAQKGMFNIFLTVFFATLAFAFVEPYF
                     FIGYLISIALFGLYQAVFMANAGGAWDNAKKIVETELKMKNTPLHAATVVGDTVGDPF
                     KDTSSVALNPVIKFTTLFGLLAVELAVSLGRDLGQGVIHGLAAVFLLISMAFVYRSFY
                     GMRIGGSTD"
BASE COUNT          560 a          945 c          906 g          508 t
ORIGIN      
        1 cggactgggc aatcgccgga tacgtgggat tgacccgttt gagctgctgg ggcggcttga
       61 tattgccgcc gacgcgaacc ggcgcctgag ggggcggcgg gggcggcggg ggcgcttcgg
      121 gcaatccacc aacaacaccg ccgacgacgc cgccgggcac gccgccctca acgccgacgc
      181 tacggtcgaa attcgtgtcg atgttggttt ctggcttgat ctcggccggc gcttcggtcg
      241 gggccgcgtt ggggttcacc acttccaccg gcttcggcgc ctgcgctgcg ggcggcggcg
      301 gcggcggggg tggcggcggc ggcggcggtg cagccgccac gaacgcgctc atcgaatctg
      361 cgggcgtcgg aatgacgtca tacgccatca aaggaatgat gacgatcgca gcgataatcg
      421 cggcttccag cagaatggtc atcgccaccg acgccccctg cttgttcccc atcttgatcg
      481 agggatcgac gatatcgcca aacatgcgtt cttcgcgtgg caccggcctt actcctgttg
      541 gctgccgcgg gaggtcgtcc cgcggggttt tcccatccgc gagacggggc tcgcagctgt
      601 cataacgttt gggcgcttat accagtgatt atttccggct gcagagcggc cgattatatc
      661 taggcccaaa aaacagtgtc aacgaaccga cgcccaacaa tagggctacc ggtcctcaac
      721 ttagacaccg atacgccgaa agtgctccgt gtctgcgccc tggagaacgc ggcattgtat
      781 cggccgtcca gggcatgcac aaggggccgt gcctcaggtt ttccccccgg acgacccttc
      841 cttaatctgt gctgccgccg atgcgcatcc cgtagaacga ccggtagacg aacgccatcg
      901 aaatcagcag gaacaccgcg gccagaccgt ggatcacacc ctgcccgagg tcgcggccga
      961 ggctcaccgc caactccacc gccagcaacc cgaacagcgt ggtgaacttg atcaccgggt
     1021 tcagcgccac cgacgaggtg tccttgaacg gatccccgac cgtatcgcca acgaccgtcg
     1081 ccgcatggag cggcgtgttc ttcatcttga gctcggtttc gacaatcttc ttcgcgttgt
     1141 cccaggcacc accggcgttc gccatgaaca ccgcctgata cagcccgaag agcgcgattg
     1201 agatcagata gccgatgaag aagtacggct cgacgaacgc gaacgccagc gtggcgaaga
     1261 agacggtcag gaagatgttg aacatcccct tctgcgcgta ttgggtgcag atctcgacca
     1321 ccttcttact gtcttcgacg gaggccttgg tgaccgtgtc gtccagcttg atattcgcct
     1381 tgatgaactc gacggcccgg tacgcaccgg tcgtcaccgc ctgcgtggac gcgccggtga
     1441 accagtagat aatcgcgccg ccggtgacca gcccgagcag gaacggggca tggagcaacg
     1501 acaggttcac cgccagctcc ggcttcagcc cctcggtcag cgccacgata atcgagaaga
     1561 tcatcgtcgt cgccccgacc accgcggtgc cgatcagcac cggcttggcg gtcgccttga
     1621 aggtgttgcc ggcaccgtcg ttctcttcga gcagatcctt cgccttgtcg aactgcacgg
     1681 tcaggccgaa atccctcttc agctcctggt gaatattcgg cacctgctcg atgaccgaca
     1741 gctcgaacac ggactgcgcg ttgtcggtga ccgggccgta ggaatcgacg gcgatcgtca
     1801 ccgggcccat gccgaggaac ccgaacgcca ccaggccgaa cgcgaacacg gccggggcca
     1861 gcatcaggtc ggacatcgcc gcgccggacg cgctcacgaa ataggcgatc gacatcagcg
     1921 cgacgatcgt caggccgagc cagtaggcgc tgaagttgcc cgcgacgaag ccggccagga
     1981 tgttcagcga cgcaccgccc tcgcgcgacg cggtcaccac ttctttcacg tggctggact
     2041 cgaccgaggt gaagaccttg acgagttccg ggatgatcgc gcccgccagc gtgccgcacg
     2101 tgatgatcga cgccagcttc caccagagcg tgccatcact gccgagatcg gcgatcagca
     2161 ggttggaaac gatgtacgtg agcacgaccg agacgatcga ggtcagccag accagcgagg
     2221 tcaacggcgc ctcgaaattc atcttggtcg cgttgccgta acgcgccttc gcaatcgcct
     2281 cgttgatgaa gtacgacccg gcgctggcga tcaccatcat gatgcgcatg gagaagatcc
     2341 agacgagcag cttcccctgc accgccggat ccttcaccgc cagcatgatg aaggtgatga
     2401 gcgccacgcc ggtcacgccg taggtttcaa agccgtccgc gctgggtccg accgagtcgc
     2461 cggcgttgtc gcccgtgcag tccgcgatga cgccggggtt gcgggcgtcg tcttccttga
     2521 tgttgaagac gatcttcatc aggtctgacc cgatgtcggc gatcttcgtg aaaatgccgc
     2581 cggcgatgcg cagtgccgcg gcgcccagcg actcgccgat ggcaaagccg atgaagcagg
     2641 ggcccgcgta gtcacccggt atgaacagca ggatcagcag catgatcagc aactccacgc
     2701 tgatcagcag catgccgatg ctcatgcccg cgcgcagcgg aatcgcatag accgcgtagg
     2761 gcttgccttc gaggctcgcg aatgccgtcc gcgagttggc gtaggtgttc acgcggatgc
     2821 cgaaccaggc cacgctgtag ctgccgagaa tgccgatcac gctgaacccc aggatgatcg
     2881 gcaacgtggt ggcgaccgcc ttgccgggaa tcgggttca
//
LOCUS       DATIMH010000369         1722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1243326, whole genome shotgun sequence.
ACCESSION   DATIMH010000369 DATIMH010000000
VERSION     DATIMH010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243326"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..1184)
                     /locus_tag="VLI90_06400"
     CDS             complement(342..1184)
                     /locus_tag="VLI90_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HSV13871.1"
                     /translation="MIRREVGSDFFLIAQNDHAILAGQLAQHFGNDRFARPEPRESVL
                     TGVRMHDAGWPLHDDEPTLNADHRPLDVFETPRPIGLKVWAASAERAAAVDPYAGLLV
                     SLHSLALSIFATSPSTPGRHERFDVSQLSERFEVNKFQHREVERQEQLRKQLGLPSDL
                     PLTHGLAEPHASADDDRLAFHFRMLQAMDLFSLCVCCTKPPADKTQDVFPKPGATATH
                     LKLRRDEKHNALRVDPWPFDQPRLELSVPYRRVPARAYQGPAELHRQLAAAAAEQLVV
                     IVTE"
     gene            complement(1291..>1722)
                     /locus_tag="VLI90_06405"
     CDS             complement(1291..>1722)
                     /locus_tag="VLI90_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HSV13872.1"
                     /translation="HSIAQRLVELETELDTLFEEHKPDVCAVEQLYAHYAHPRTAIMM
                     AHARGVILLVAARREIRVEQFPANRIKQSVTGHGHASKEQMQRAVQSLWNLKDPPEPP
                     DVADALSVALCCGRHLEMVKLRGDTTPLIAAVPDDSEDDDR"
BASE COUNT          288 a          479 c          625 g          330 t
ORIGIN      
        1 atcaaaagat ggcgcgggtt catgagtgaa tatggtaacg cgacgcggtg tgtggcgaaa
       61 tccagaggac ggcggcgaca gcggcgggct ttcgcgttcc ggagccccgg atgttcacgc
      121 tgaggcgacg ctgttgcatg aatggttagc aatcaaccac gccgggttcg gaggtactcc
      181 gacgacccgg atgttttcgc gcaagcgccg cagaatccgg gtcgtcggag tatccttcga
      241 cttcgctcgg gacaggcctc cgaacccggc gtgaatggtt caagaccgct tgtgcgaaag
      301 tgtcggatcg tcagcatgta catgcggggc ttcgcgggga gtcattccgt gacaatcacg
      361 acgagttgct cggcggccgc ggcggcgagt tgcctgtgta gttctgcggg gccctggtac
      421 gcacgcgccg gtacgcggcg gtacgggacg ctcagttcca gccgcggttg atcgaacggc
      481 cagggatcga cgcgcagcgc gttgtgcttt tcgtcgcggc ggagtttcag gtgcgttgcc
      541 gttgcgccgg gcttgggaaa cacatcctgt gttttgtccg ccggcggttt cgtgcagcac
      601 acgcaaaggc tgaacagatc catcgcctgc agcattcgga agtggaaggc gaggcgatca
      661 tcatcggccg acgcgtgcgg ctcggctaag ccgtgcgtca gcggcaggtc gctcggcagg
      721 ccgagctgtt tgcgcaactg ctcctgtcgc tcgacttcgc ggtgttggaa tttgttcacc
      781 tcgaagcgct cggacagttg gctgacgtcg aagcgctcgt gccggccggg cgtgctgggg
      841 gaggtggcga agatgctgag cgccagcgag tgcagactga cgagcaggcc cgcgtacgga
      901 tcgacggcgg cggcgcgctc ggcggaggcg gcccagactt tgaggccgat cggccgcggg
      961 gtttcgaata cgtccagcgg gcgatggtcg gcgttcagcg tgggctcgtc atcgtgcagc
     1021 ggccagccgg cgtcgtgcat gcgcacgccg gtgagaacag actcgcgcgg ctcggggcgc
     1081 gcgaagcgat cgttgccgaa gtgctgcgcc agctgccccg ccaggattgc gtggtcgttc
     1141 tgggcgatga gaaaaaagtc gctgccgact tcgcggcgga tcatgggagt agggtaacgg
     1201 ggagcggatt agagcgacaa gctttgaaag attcgccgct tgcggcttag cagatcggcg
     1261 ccgctgggtt tgcgaagccg caagcggcga tcaccgatcg tcatcttccg aatcgtcggg
     1321 aaccgctgcg atgagcggcg tcgtgtcgcc tcgaagtttc accatctcca gatgccgtcc
     1381 acaacacagc gcaacagaca gggcgtcggc gacgtcgggc ggttccggcg gatcctttaa
     1441 attccacagc gactggacag cgcgctgcat ttgttctttg ctcgcgtgac cgtggccggt
     1501 gacggattgc ttgatgcggt tggcgggaaa ctgttcgacg cgaatctctc gccgggcggc
     1561 gaccagcaga atgacaccgc gggcgtgggc catcatgatc gcggtgcgcg ggtgggcgta
     1621 gtgggcgtag agctgttcga cggcgcagac gtccggcttg tgttcttcga agagcgtgtc
     1681 aagttcggtt tcgagttcga cgaggcgctg ggcgatcgag tg
//
LOCUS       DATIMH010000370        10437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5285710, whole genome shotgun sequence.
ACCESSION   DATIMH010000370 DATIMH010000000
VERSION     DATIMH010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5285710"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1541)
                     /gene="thrC"
                     /locus_tag="VLI90_06410"
     CDS             complement(63..1541)
                     /gene="thrC"
                     /locus_tag="VLI90_06410"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSV13873.1"
                     /translation="MNDAAFQQCINPACGQTFAVEEVKVACTRCGSLLDIKYDWSRLP
                     ALKSLNYFEHRWATKGTETEGRIDFSGVWRFRELFPFYRTESDIVTIGEGRTTLQTAD
                     LLAKQMHMRPGHLLLQYEGLNPSGSFKDNGMTAAFTHARMVGAHKVACASTGNTSASL
                     AMFASLAEMEGIVFIGSGKISFGKLSQALDYGAKTLQIQGDFDACLRRIRQIAIDPRM
                     GVYLMNSVNPFRLEGQKSIMLRILEARDWEPPDWIIVPGGNLGNCSAFAKAYMELKEL
                     GLVKKVPRLAVINAAGANTLNVLVNDRKLTWQGGNFDPTIIQKHYAVLDESNYYPHTI
                     ASAIEISRPVNLPKALRALEFMNGVVREVTDEEILEHRAMVARWGFGCEPASAASVAG
                     LRKLLEEQVISADESVVCILTGHELKDPNATVKYHTGVDMKVAQDLAPRETPHGKLSN
                     PPVQVADDLKAIVRALGVDPDKVDLSAYNASAAKPGVPVAEF"
     gene            complement(1694..1975)
                     /locus_tag="VLI90_06415"
     CDS             complement(1694..1975)
                     /locus_tag="VLI90_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13874.1"
                     /translation="MLAAAGLIVFFIFAGFDALTNLEADLAAALRPLFTMERDAAAAA
                     LAGDAWDEGFLLAGLPIGFLLGASFFDDVLATVTRRSFRARQTSDNCLN"
     gene            2187..2486
                     /locus_tag="VLI90_06420"
     CDS             2187..2486
                     /locus_tag="VLI90_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HSV13875.1"
                     /translation="MEREALRSGGTSNLSTLPIHMADMGTDNYEQEFTLGLVEKDRNL
                     LREINNALAKIQNGTFGICEGTGKPIPKARLEVQPWARFGVEYQSRMEKGTMGVR"
     gene            2749..3330
                     /gene="lspA"
                     /locus_tag="VLI90_06425"
     CDS             2749..3330
                     /gene="lspA"
                     /locus_tag="VLI90_06425"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HSV13876.1"
                     /translation="MPRSSDFAITPAAIARFAVTTAAGLSLDLWSKAQAVVHLKNADP
                     FDFIPGWLQFEYTENHGAVFGVAQGQRWVFLVVSFLALVFLTYLFSASGRRAFYQIIL
                     GMLLAGVLGNMYDRIAFGYVRDMIHALPMWPHFFPWIFNVADSLLCVGVGLMLVYSFF
                     HSPPPSPSVPAEGRRPIEPRPHDERSPIVSENP"
     gene            3434..4597
                     /gene="ribD"
                     /locus_tag="VLI90_06430"
     CDS             3434..4597
                     /gene="ribD"
                     /locus_tag="VLI90_06430"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HSV13877.1"
                     /translation="MKRILDEHYLRRAIRLAMNGRGRVEPNPMVGCVIVKNDRVIGEG
                     YHAHFGGPHAEPSALANCTESPEGATGYVTLEPCCHTNKKTPPCAPRVIAAKIARVVV
                     GCLDPNPQVNGNGVRMLRDAGIDVVGPMLEAEAKQLIAPFLARVTHHRPYVTLKWAES
                     ANGLVAGRMGRPVRITNATSDRAVHALRARCDAIAVGTNTVLNDDPLLTARDVQTHRP
                     LLRVVLSNTLKITAQSRLVQTAREQPLLVFCSHESAAQHSERIGELKRAGAEVIALDT
                     AGEGRFAFPDVLAELHHRAVTHLLIEPGPTLARALIEREQADRVWVIRSSKTIEDADG
                     LPAAAVPYPAVGEVTLDGDRLTEYLNPASAVFFAPAASADFVLTEEQSSPLKR"
     gene            complement(4608..5411)
                     /gene="purQ"
                     /locus_tag="VLI90_06435"
     CDS             complement(4608..5411)
                     /gene="purQ"
                     /locus_tag="VLI90_06435"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HSV13878.1"
                     /translation="MKPKTLILRTAGTNCDGETAYAFERAGATAAFLHINRLLERPQL
                     IHDYQILAFPGGFSYGDDIAAGRILANQITHHLRDVLQAFVDAGKPVIGICNGFQVLV
                     KTDLLPGALAGQTGQTATLTNNDSGRFIDRWIHLAPRGSKCIWTAGLEPLDLPIAHGE
                     GKFVPVNEQVRRALADNDQIALVYAKADGSPAAGQSPENPNGSIDDIAGVCDASGLVF
                     GLMPHPERYVEPFQHPAWTRQQPLPGVGRGLKFFENAVRHASEAVGAGV"
     gene            5555..8899
                     /locus_tag="VLI90_06440"
     CDS             5555..8899
                     /locus_tag="VLI90_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13879.1"
                     /translation="MRTHVTAIILFIAAAFGLQASPPTNGAEIQAKFDPPRGVVKDDQ
                     GRAIDGATVVVYCVHSRWGLGNKTIETVTSDAAGNFVLAQPLTFENLSGTDYTDFYLL
                     YAKKAGFAAGWAQIFSNGPAASYELTLMPSKSQLVHVTDRATGQPIKGAAVFLYSAGT
                     PVDHEAHFRRPFFTSTNSGMAEATTDEQGKATISGVPDTTCGFRTTCSGYAPGSPVAA
                     GSRISPVPAAIQLTAAATVSGVLQDDSGAPIADAILAFSPTDPISHYTLAKTASDGTF
                     VVDDLIPKGGFPFANTSGSGDYNVEVRSDRFTAPRRTVHVDPGQWIEGFDITASPGVL
                     IKGKLIDVDGKPVVGGRVQGDSPSGRLDSYSDRAGTFRWRVMPGQLNAFVVSPPGGTY
                     MVDGNRASQQMTLTAATKEIDLTLRIEGKLGRLVSAHGRLLRPEGTPAAGVAVLPSTD
                     ERVSTASTYGSFRQAITAADGTFTFDEVPVDLPLQVYADAEKRKLVFSATVPPPALPA
                     GEVAKELDLGELHMIAGQTVTIHVTDLAGKPRGGLSLLMTPETCPQRTRAVRSGADGN
                     VHVDGIIPDLRYSIQDRSGGGRGSFQTTLVPLPKDGGGAAVNLVVDPAIAVHVVGPDA
                     KPILIGDITQISVTIPEGAWQLGPKPQIVGHLDDGSVTVSREQFVLGKPGAHVEIRYT
                     VADGSHGVAVGQMPSDGAGVIELKTMGGSAGVDFTNGPAAAHDEIAARIVDPDGKPLA
                     DVEVSLLPQDFRAPRPPSRTNADGVVRFPGIQHDYQRYFQIAKAGFGTRWLVDPPMGK
                     GITVKLDNTTRFRGTLHGVDGSTLTSAATIMLITSRPTVRANYGDVGPLMITVVSDEH
                     GAYDVPIEPGEYEARVTAPAGPGGTAVFLNAQHLQIAAGQVQELPGQLGPGADIKFLA
                     IDETTGKPVAGVKFFIERSSYGRADMQPGSERTSDEQGVVHYESLMPTLTQFEPWLAG
                     YTRWWCDEDAWFRGRAPHGIDAVNLKLAPGAHTYTIHMQRGMHVFGKVITPNGESVDR
                     VWIEIEGLNTGDQRYAPRAKANGEFETWLPVGLRNQPVTTVSLKATDQQRGWTATSEQ
                     FQCTVGGERSVTLKLAAEGSGPATQP"
     gene            9208..10398
                     /locus_tag="VLI90_06445"
     CDS             9208..10398
                     /locus_tag="VLI90_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HSV13880.1"
                     /translation="MSTATSNVSDIPENADIDRDTPQPQIHVEERKLTLQDFLKTVVK
                     INGSDLHLQAGSVPMIRVDGRARFLDCPAPDDQQMKEYVDAIIKSQGEPEDRRHQLEH
                     KGAVDVAYSIAGLARFRTNIFHSREKYAIVMRRIVTKIPNFEDLSLPPIVEGLADFHR
                     GIVVVAGTTGSGKSTTLAAIIGKINRSRSERIITVEDPIEFQHENAKALVSQVEVGTD
                     SESYEYALRAMMRQDPDTILIGEIRDPFSLSTALKASDTGHLVYTTIHATNAWTSVQR
                     IVSLFDPNMRELQQQQLAMNLNAIICQRLAKKREGKGRVPVVEIMMATPLVRKYILDG
                     EFDKLKSTIGNKEAGSQSFDQHLTELFHRQVIDINEASRLASNVDALKLALRGITNSD
                     TRLK"
BASE COUNT         1976 a         3298 c         3307 g         1856 t
ORIGIN      
        1 tgtggcgccg cctgctgcag gtgtcatggg acctcctgaa ccgcagtagt ttgtccgatc
       61 gatcaaaact ccgccaccgg cacgcccggc ttcgctgcgc tggcattgta cgcgctcagg
      121 tccaccttat ccggatccac acccagcgcg cggacgatcg ctttcagatc atccgctacc
      181 tgcaccggcg ggttcgacag cttgccatgc ggagtttcgc gcggggcgag gtcttgcgcg
      241 accttcatgt ccacgccggt gtgatatttc accgtcgcgt tggggtcttt cagctcgtga
      301 ccggtgagga tgcacacgac cgattcatcg gccgagatga cctgctcctc gagcagcttg
      361 cgcagccccg cgacgcttgc ggcgctggcg ggttcgcagc cgaagcccca cctcgcgacc
      421 atcgcgcggt gctcgaggat ctcctcgtcc gtcacctcgc gcaccacgcc gttcatgaac
      481 tccagcgcgc gcagcgcctt cggcagattt acaggccggc tgatctcgat cgcgctggcg
      541 atcgtgtgcg ggtaatagtt cgattcatcc agcaccgcgt aatgcttctg gatgatcgtc
      601 ggatcgaagt tgccgccttg ccaggtgagc ttgcggtcgt tcacaagaac gttcagcgtg
      661 ttggccccgg cggcgttgat gaccgccagc cgcggcactt ttttcaccag gcccagctcc
      721 ttcagctcca tgtacgcctt ggcgaacgcc gagcagttcc ccaaatttcc gccggggacg
      781 atgatccaat ccggcggttc ccagtcgcgc gcttccagga tgcgcagcat gatgctcttc
      841 tgcccttcga ggcggaaggg gttcacgctg ttcatcaggt agacgcccat gcgcgggtcg
      901 atggcgatct gccggatgcg ccgcaggcac gcgtcgaagt cgccctggat ctgcagcgtc
      961 ttcgctccgt agtcgagcgc ttgcgacagt tttccaaaac tgattttccc ggagccgatg
     1021 aagacgatgc cttccatctc cgccagcgat gcgaacatcg ccaggctcgc gctggtgttg
     1081 ccggtcgatg cgcacgccac cttgtgcgcg ccgaccattc gcgcgtgcgt gaacgcagcg
     1141 gtcatgccgt tgtctttgaa gctaccggat ggattcaagc cttcgtactg cagcagcagg
     1201 tgacccggcc gcatgtgcat ctgcttggcc agcaggtcgg cggtctgcag cgtggttcgg
     1261 ccttcaccga tcgtgacaat gtcgctttcg gttcgataga acgggaatag ttcacggaag
     1321 cgccacacgc cggaaaagtc gatccggcct tcggtctcgg tccccttcgt ggcccagcgg
     1381 tgttcgaagt aattgagcga cttgagcgcc ggcaggcgcg accagtcgta tttgatgtcg
     1441 agcaggctgc cgcaacgggt gcaggcgacc ttcacttctt cgacggcaaa agtctgcccg
     1501 catgcggggt tgatgcactg ctgaaaggcg gcgtcgttca tcgtccgcac aaggtaggat
     1561 tgcgcggcgc aaaggtcaaa acgcgctgtc gaaggtccag taccggcctt actttacggc
     1621 cgatcgggta ttgacaacgg acacaaaata agttagtatc ggctgttctc tgaacttcgt
     1681 ggcgcaagtt gggttaattg aggcagttat cagacgtttg gcgggctcga aaggagcggc
     1741 gggtcaccgt ggctaagaca tcatcgaaga agctcgcacc gagcaaaaag ccgatcggta
     1801 aaccggccag caagaaaccc tcgtcccacg cgtcccccgc caaggccgcc gccgcggcgt
     1861 cgcgctccat cgtaaagagc ggccgcaacg ccgccgccaa gtcggcttcc aaattcgtca
     1921 aggcatcgaa gcccgcgaat atgaaaaata caatcaaacc ggctgctgca agcaagcccg
     1981 cggtggtcgt cgcctagaag ggcggcaagc cccatcacgc cagcgacgct ccgcaactga
     2041 agcccgaagg cacaccgctg ccggatcgag tcgcgacgaa cctgaagccg cgaaaaaacc
     2101 aggccggctt caactccaaa gagctggaac atttccgcga caagctgctg gaaaaacgcc
     2161 gcgagatcgt cggtgacatg agctcgatgg aacgcgaagc cctgcgctcc ggcggcacca
     2221 gcaacctgtc caccctgccc atccacatgg ccgacatggg caccgacaac tacgaacagg
     2281 aattcacgct gggcctggtc gaaaaggacc gcaacctgct ccgcgagatc aacaacgccc
     2341 tggccaaaat ccagaacggc accttcggca tctgcgaagg caccggcaag ccgatcccca
     2401 aagcgcggct ggaggttcaa ccttgggcgc gcttcggcgt cgaatatcaa agccggatgg
     2461 aaaagggcac gatgggggtt cggtagaaat tccgaatggc gaagtccgaa tccccgaatc
     2521 aatgacgaat gacgaaatcc gaatgaagaa acgcgcgcgc gagcgcagcc gtcgccagga
     2581 ctaaccagcg gttagtgcat ttggtatttc attcgtcatt cgcacttggt cattcggaat
     2641 tcattcgggg attcgggttt cgccattcgg ctttcggatc cgagagtcat tccgggattc
     2701 gcactttcac cattcggatt ttcccccacg acttcctaca atccccgcat gccgcgttcg
     2761 tctgatttcg ctatcacccc cgccgccatc gcccgctttg ccgtcaccac cgccgcgggg
     2821 ctgtcgctgg acctttggtc caaagcccag gccgtcgtgc acctgaagaa cgccgatcca
     2881 ttcgacttca tccccggctg gctgcagttc gaatacaccg agaaccacgg cgccgtcttc
     2941 ggcgtcgccc aaggccaacg ctgggtcttc ctcgtcgtct cgttcctggc cctggtgttc
     3001 ttaacctacc tcttctccgc cagcggccgg cgggcgtttt accagatcat cctcggcatg
     3061 ctgctggccg gcgtgctggg caacatgtac gaccgcatcg cgttcggcta cgtccgcgac
     3121 atgatccacg cgctaccgat gtggccgcat ttcttcccgt ggatcttcaa cgtcgcggat
     3181 agtctgctct gtgtgggcgt ggggctgatg ctggtgtaca gcttcttcca ttccccgccg
     3241 ccttcgccat ctgtgccggc cgaaggacgc aggccaatcg aaccgcgacc gcatgatgag
     3301 cgctcgccga tcgtatctga aaatccatga tagccggggc cggaaggccc ggcgtgttcg
     3361 accgaaagag accggggctt ccggcctcgg ctattacagc gcccacatcc gacgcactac
     3421 aatcacgcat cgaatgaagc gcattctcga tgagcattac ctccgccggg cgatccgcct
     3481 cgcgatgaac ggccgcgggc gtgtcgagcc caacccgatg gtcggctgcg tgatcgtcaa
     3541 gaacgaccgg gtgatcggcg aaggctatca cgcgcacttc ggcggaccac acgccgagcc
     3601 aagcgcgctg gccaactgca cggaatcccc cgaaggcgcg acggggtacg tgacgctgga
     3661 accgtgctgc cacacgaaca agaagacccc gccgtgcgcg ccgcgggtga tcgcggcgaa
     3721 gatcgcccgc gttgtcgtcg gctgtctcga tccgaacccg caagtcaacg gcaacggcgt
     3781 gcgcatgctg cgagacgccg gcatcgacgt cgtcggcccg atgttggagg ccgaagcgaa
     3841 gcagctgatc gcgccgtttc tggcgcgcgt gactcatcat cgcccgtacg tcacgctgaa
     3901 gtgggccgag tccgccaacg gcctggttgc cgggcgaatg ggccggccgg tgcggatcac
     3961 gaacgcgacg tccgatcgcg cggtgcacgc gctgcgcgcc cgctgcgatg cgatcgccgt
     4021 cggcacgaac acagtactga atgatgaccc gctgctgacc gcgcgagacg tccagacgca
     4081 ccgcccgctg ctgcgggtcg tgctatcgaa cacgctgaag atcacagcgc aaagtcgatt
     4141 ggtgcagacc gcgcgcgagc aaccgctgtt ggtcttctgc tcgcacgagt ccgcggccca
     4201 gcattctgag cgcattggcg agttgaagcg cgccggcgcc gaagtcatcg cactcgacac
     4261 cgcgggcgaa ggccggttcg cgtttccaga tgtgctggcg gaactgcatc atcgcgcggt
     4321 gacgcacctc ctgatcgagc cggggccaac cctggcgcgg gcattgatcg agcgcgaaca
     4381 ggcggatcgg gtttgggtga ttcgatcatc aaaaacaatc gaagacgcgg acggcctgcc
     4441 cgcggcggcc gtgccgtatc cggcggtggg ggaagtcacg ctcgatgggg atcggttgac
     4501 ggagtatctg aatccggcga gcgctgtttt cttcgcgccg gctgcttcag cggattttgt
     4561 tttgaccgaa gagcagagtt caccgctgaa gcggtaagcg tggttattca cacgccggcg
     4621 ccgacggctt cgctcgcgtg gcgcaccgcg ttttcgaaga acttcaaccc gcgacccacg
     4681 cccggcagcg gctgttgccg cgtccacgcc ggatgctgga acggctcaac gtagcgctcg
     4741 ggatgcggca tcaggccgaa gaccaggccc gatgcatcgc acacgcccgc gatgtcgtcg
     4801 atgctgccgt tcggattctc cggcgactgt cccgcggcgg gggagccgtc cgctttcgcg
     4861 tacaccagcg cgatctgatc attgtccgcc agcgcccggc ggacctgctc gttcaccggc
     4921 acaaattttc cttcgccatg cgcgatcggc agatcgagtg gctccagccc cgccgtccag
     4981 atgcacttgc tcccgcgcgg cgcgaggtga atccaccgat cgatgaaccg cccgctgtcg
     5041 ttgttggtca acgtcgcggt ctgccccgtc tgtcccgcga gcgcacccgg cagcaggtcc
     5101 gtcttcacca ggacctgaaa gccattacag atgccgatga ccggcttgcc cgcgtccaca
     5161 aacgcctgca gcacgtcgcg caggtggtgg gtgatctggt tcgccaggat ccgccccgcg
     5221 gcgatgtcgt cgccgtaact gaagccgccg gggaaggcga ggatttgata atcgtgaatc
     5281 agctgcggcc gctcgagcaa gcggttgata tgcaggaacg ccgccgtcgc gccggcccgc
     5341 tcgaatgcgt acgcggtctc cccatcacaa ttcgtcccgg cggtgcgaag gatcaatgtt
     5401 ttgggcttca tccgctgaag tgtaggcatc acgccgcgga attaaaggtt gcggcgggaa
     5461 gggccttcgc atggtgaaaa agtggctcgt cgtcggctgc gcgagattgg aagattttcc
     5521 ctgccggccg tcgcgtgagg gtataaacgg tggcatgcgt acgcatgtca ccgcgatcat
     5581 tttgttcatc gccgccgcgt ttggcctcca agcttccccg ccgacgaacg gcgcggagat
     5641 tcaggcgaag ttcgacccgc cgcgtggcgt ggtgaaggat gatcagggcc gcgccatcga
     5701 tggcgcgacg gtcgtcgttt actgcgtgca tagccgctgg ggactgggga acaagacgat
     5761 cgaaaccgtg accagtgacg cggcggggaa ttttgtcctc gcgcagccgc tcacgtttga
     5821 gaacctcagc ggaaccgact acacagattt ctacctgctg tacgcgaaga aagccggctt
     5881 cgccgcgggg tgggcgcaga tcttcagcaa tgggccggcg gcgtcgtacg agttgacgtt
     5941 gatgccgagc aaatcgcagc tggtgcacgt gaccgatcga gccacgggcc agccgatcaa
     6001 aggggcggcg gtttttctgt actccgcggg gacgccggtc gatcacgagg cgcatttccg
     6061 ccggcccttt ttcacgtcca ccaacagcgg gatggccgaa gcgaccaccg acgaacaagg
     6121 gaaagcgacc atcagcggcg tgccggatac gacctgcggg tttcgaacca cgtgcagcgg
     6181 gtacgcgccg ggcagtcccg tggcggccgg cagccgtatc tctccagttc cggcggcgat
     6241 ccagctgacc gccgcggcaa cggtgtctgg tgtgctgcag gatgactcgg gcgcgccgat
     6301 cgccgacgcg attctcgcgt tctcgccgac cgatccgatc tcgcattaca cgctcgcgaa
     6361 gaccgcgagt gacggcacat tcgtcgtcga tgatttgatt cccaaaggag gcttcccgtt
     6421 cgccaatacg tccggcagtg gtgactacaa cgtggaagtg cgcagcgatc gcttcacagc
     6481 gcctcgccgc acggtgcacg ttgacccggg gcaatggatc gaggggttcg acatcacggc
     6541 ctcgcccggc gttctgatca aaggcaaact gatcgacgtt gacggcaaac ccgttgtcgg
     6601 cgggcgcgtg caaggcgata gcccgtcggg tcggcttgat tcctattccg atcgtgccgg
     6661 cacattccgc tggcgggtga tgccggggca gctgaacgcc ttcgtggtct cgccaccggg
     6721 cggcacgtat atggttgacg gcaatcgcgc cagccagcag atgacgctga cggcggcgac
     6781 gaaggaaatc gatctgacgc tgcggatcga agggaagctc ggacggctgg tgtcagcgca
     6841 cgggcggttg ctccgccccg agggcacacc ggcggcaggc gtggccgtgc tgccttcgac
     6901 ggacgagcgc gtatcgacgg cgagtaccta cggctcgttt cgccaagcga tcaccgcggc
     6961 cgacggcacg ttcacgtttg acgaagttcc ggttgatctg ccgctgcagg tatacgcgga
     7021 tgcggaaaag cgaaagctgg ttttttcagc caccgtgccg ccgccggcac tgcccgcggg
     7081 agaagtagcg aaggaattgg accttggcga acttcacatg atcgccggtc aaacggtgac
     7141 gatccacgtc acagatctgg cggggaaacc gcgcggcggg ctttcattgc tgatgacgcc
     7201 ggagacctgc ccgcagcgta cgcgcgccgt gcgcagcggc gcggacggca acgttcacgt
     7261 tgacgggatc attcccgatc ttcgctattc gattcaggat cgcagcggcg gcggccgcgg
     7321 gagttttcag acgacgttgg tgccgctgcc gaaggatggc gggggcgcag cggtcaatct
     7381 ggtggtcgat ccagccatcg cggtgcacgt ggttggaccg gacgccaagc cgattctgat
     7441 cggcgacatc acgcagatca gcgtgacgat tccggaaggc gcctggcagc tggggcccaa
     7501 accgcagatc gtgggccacc tcgacgacgg cagcgtaacg gtctcgcgcg agcagtttgt
     7561 gctgggcaag cccggggcac acgtcgagat tcgttacacc gtcgccgatg ggtcgcatgg
     7621 cgtagcggtc ggccagatgc ccagcgatgg cgcaggtgtg atcgagctga agacgatggg
     7681 cggctcggcc ggcgtcgatt tcaccaacgg ccccgcggcg gcgcatgacg aaatcgcggc
     7741 gcgcatcgtc gatcccgacg gcaagccgct ggcggatgtc gaggtgtcgc tgttgccgca
     7801 ggatttccgc gcgccgcgcc caccgtcgcg cacgaacgcc gacggcgtcg tgcgctttcc
     7861 cggcattcag cacgactatc agcggtattt ccagatcgcc aaggccggct tcggcacgcg
     7921 ctggttggtt gacccgccga tgggcaaggg gattacggtg aagctcgaca acacgacgcg
     7981 ctttcgcggc acgctgcacg gcgtcgatgg ttcgacgctg acgtccgcgg cgacgatcat
     8041 gctgatcacc agccgcccga ccgtgcgggc gaactatggt gatgtggggc cgctgatgat
     8101 taccgtggtc agcgacgagc acggcgcgta tgacgtgccg atcgaacccg gcgaatacga
     8161 agcgcgggtc acggcacccg ccgggcccgg cgggacggcg gtgttcttaa acgcccagca
     8221 tctgcagatt gccgcggggc aggtgcagga attgccgggt cagcttggcc cgggagctga
     8281 tatcaaattt ctcgcgatcg atgagacgac gggcaagccc gttgccggtg tgaagttctt
     8341 catcgagaga agctcgtacg gccgggccga catgcagccc ggcagcgagc gaaccagcga
     8401 cgagcagggc gtcgtacatt acgaatcgct gatgccgacg ctcacgcagt tcgagccgtg
     8461 gctcgccggc tacacgcgct ggtggtgtga cgaagacgcc tggttccgcg gccgcgcgcc
     8521 gcatggcatc gacgcggtga acctcaagct cgcccccggc gcgcacacgt acaccatcca
     8581 catgcagcgc ggcatgcacg tcttcggcaa agtgatcacg cccaacggcg aatccgtgga
     8641 ccgggtctgg atcgagatcg agggcttgaa caccggtgac cagcgctatg cgccgcgtgc
     8701 caaagccaac ggcgagttcg aaacctggct gccggtcggc ctccgaaatc agccggtgac
     8761 gacggtctcg ctgaaggcga ccgatcagca gcggggatgg accgcgacca gtgagcaatt
     8821 tcaatgcacc gtcggcggcg agcgctcggt cacgctgaaa cttgctgccg agggttcagg
     8881 tcccgcgacg caaccgtagc atgaaaagat ctgacaggcg ggacaatcgg atgcggttcc
     8941 ttccccctct cccggtacgc cgggagaggg tcggggtgcg ggtgattgga gtaccaggtg
     9001 ctttgaaatt ccaaatcacc ctcaccctag ccctctcccg gcgtaccggg agaggggaca
     9061 agagcgcgca ttcgtgacat gtgttttccc tgcgcgatgc gaacctgcga caagaatccc
     9121 gttgccattc cggcggcgtc gaacaataat cgtggcgtcg tttgcacccg agtcagtcac
     9181 cgccggattg tgtgaggaac ccaccctatg tctaccgcca ccagcaacgt cagcgatatt
     9241 cctgaaaacg ccgacatcga ccgcgacacg ccgcaaccgc agattcacgt ggaagagcgg
     9301 aagctgacgc ttcaggattt tctgaagacg gtggtgaaga tcaacgggtc ggacctgcac
     9361 ctgcaggcgg gcagcgtgcc gatgatccgc gttgatggcc gggcgcggtt tctggattgt
     9421 ccggcgcccg acgaccagca gatgaaggaa tacgttgacg cgatcatcaa gagccagggc
     9481 gaaccggaag atcgccggca tcagctcgag cacaagggtg cggtggacgt ggcgtacagc
     9541 atcgccgggc tggcgcgctt ccgcacgaac atcttccaca gccgcgagaa gtacgcgatc
     9601 gtcatgcggc ggatcgtgac caagatcccc aacttcgaag acctcagctt gccgcccatc
     9661 gtggaaggcc tggcggattt tcaccgcggc atcgtcgtcg tcgccggcac gaccggttcg
     9721 ggtaaatcga cgacgctcgc cgccatcatc ggcaagatca accgctcgcg cagcgagcga
     9781 atcatcacgg tcgaagaccc gatcgagttc cagcacgaga acgccaaggc gctggtctcg
     9841 caggtggaag tgggcaccga cagcgagagc tacgaatatg cgctgcgcgc gatgatgcga
     9901 caggacccgg acacgatcct gatcggcgaa atccgcgatc cattttcgct gtccaccgcg
     9961 ctgaaggcct ccgacaccgg ccacctggtc tacacgacga ttcacgcgac caacgcgtgg
    10021 accagcgtgc agcgtatcgt gtcgctgttc gacccgaaca tgcgcgagct gcagcagcag
    10081 cagctggcga tgaacctcaa cgcgatcatc tgtcagcgcc tggcgaagaa gcgcgaaggc
    10141 aagggccgcg tgccagtcgt cgagatcatg atggccacgc cgttggtgcg caagtacatc
    10201 ctcgacggtg agttcgacaa gctgaagagc acgatcggca acaaggaagc cggcagccag
    10261 agcttcgacc agcacttgac ggagttgttc caccggcagg tgatcgacat caacgaagcc
    10321 agccggctgg cgagcaacgt ggatgctctg aagctggcct tgcgcgggat caccaacagt
    10381 gatacgcggt tgaagtagac gcgcgtttca gccgcttgcg gcttcgcaga ccaggtg
//
LOCUS       DATIMH010000371         3728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4416907, whole genome shotgun sequence.
ACCESSION   DATIMH010000371 DATIMH010000000
VERSION     DATIMH010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4416907"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VLI90_06450"
     CDS             complement(<1..294)
                     /locus_tag="VLI90_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13881.1"
                     /translation="MADEIDVVPIELRRQLQQSSILTCVTVAADEAARRTETDAGSRD
                     RHELAGRRKVVRAVVEGRGTGAVGSRAAIEARNSAGTGAGQDRAAEANRSGGRQ"
     gene            385..2466
                     /locus_tag="VLI90_06455"
     CDS             385..2466
                     /locus_tag="VLI90_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSV13882.1"
                     /translation="MTGNANFDPLKLLFSSGGSGSGATRDTNPGNREAIALNNPYLDP
                     SASSFIASQILASGVNPCNFSPLSTTDRNRIAAGTFRFCLRENLEGLGDRTEEARRDT
                     YRIVGGVKGDFNNDWHYEISVNYGHLKERTDIKGNLDTQRFLLANDAVRDPSTGQIVC
                     ASKIDPSRAFGALPWFYYQYYGADYPGADPNAPARLAKDIAACTPINPIGGNFTEAQK
                     AYLLRDTVAKGSTSQFDATAFISGNTSKFFNLWGGPVGFVLGAEYRTDNVTYNLDRDT
                     QLGYTFYNAIPSFTAPKSKVKEAFGEISLPIFKDRPFLRELEVDAAARVSDYNLGTTG
                     TVWAYNVNGIYSPFNGLRFRANYARAVRAPNQVELFSPSGQNFAPGFSDPCSAININN
                     GSQFREANCNAAGRPAGYDFRYSSSLGFKSGGNPNLKAETSDSYTAGFVMTPQFLPGF
                     ETSVDYYNIKLKNSISFVGAQAVANACYDVPTSPNVFCSQFERAGPSGGPHGEQPFQI
                     IEGSLLSAPLNYAKLTASGIDVEVAYRRQVGDIGRLDTRLTYTHVLNRSNYISATDPN
                     FEDQQLLELGDPQDAFNWNTTIQHGRFTFGYQMRYVGKMVLNLYEDFFSVQGRPPQNP
                     DYSNRQFYPAKFYHDIRLGIDVTRKYNFYLGVDNLTNTIPPYGLTGIGGGSAIYDNRG
                     RFMYAGVKANF"
     gene            2556..3512
                     /locus_tag="VLI90_06460"
     CDS             2556..3512
                     /locus_tag="VLI90_06460"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSV13883.1"
                     /translation="MIAIDSAFDLVRAGRASEAVFELESAASAGDASSALELGIWFLE
                     GKHVPRDLRRSREAFRRAAELGNASAEQVFTSFMAIGVGASADWAGALQRLRAFARVD
                     SAAAEQLALLDRMNLTQAGDPGEHPVARQLSVEPQVNCFEQLFSGDECSYLISRARPL
                     LQPSVIVEPSTGQLQPHPIRTSEGAMFPWASEDLVIHALNRRLAWASGTAVACGEPVQ
                     VLSYRPGQQYRPHLDALPNTGNQRILTMLVYLNEGYSGGETFFTRTGLKFAGKTGDGL
                     LFRNAGAGGHPDHNAEHAGLPVLRGEKFIASRWIREQPLMPH"
BASE COUNT          728 a         1162 c         1088 g          750 t
ORIGIN      
        1 ctgtcgccca ccggatcggt tcgcttcggc ggcacgatcc tggccggcac cggtgccggc
       61 actgtttctg gcctcaattg cggcccggct gccaacggca ccggttcctc ggccttctac
      121 aactgcgcgt acaactttac gccgaccggc cagctcgtgc cgatcacggg aacccgcgtc
      181 ggtctcggtc cgacgggcag cttcatcggc ggcaacggtt acgcacgtca ggatggacga
      241 ctgctgcagc tgacgccgca gctcaatcgg tacaacatca atctcatcgg ccatttcgaa
      301 gtcagcccgg cgatcgttcc gttcatcgag gccaaattct cgcgaacgga ttcctcgggc
      361 acgggatcga gcggaccggc gttcatgacc ggcaacgcca acttcgaccc cctcaagctg
      421 ctcttctctt cgggcggcag cggatcgggc gcaacccgtg acaccaaccc tggaaaccgc
      481 gaagcaatcg ctctgaacaa tccttatctg gatcccagtg cgtcgagctt tatcgccagc
      541 cagatcctgg cgagcggcgt aaacccctgt aacttctcac ctttgagcac gacggatcgc
      601 aatcgcatcg ccgccggcac attccgcttc tgccttcgcg aaaacctcga gggactaggc
      661 gaccggaccg aagaggcgcg gcgcgacacg taccgcatcg tcggcggggt gaagggcgac
      721 ttcaacaacg actggcacta tgagatctcg gtaaactacg gccatctcaa agagcgaacc
      781 gacatcaagg gcaacctcga cacccagcgc ttcctgctcg cgaacgacgc tgttcgcgat
      841 ccgagcaccg gacagattgt ctgcgcatcc aagatcgacc cgagccgcgc gtttggcgcg
      901 ctgccctggt tctattacca gtattacggc gcggactatc cgggtgccga tccgaacgca
      961 cctgcccgcc tggcgaagga tatcgcagcg tgcacgccca tcaatccgat cggcggcaac
     1021 ttcaccgaag cgcagaaggc gtatctcttg cgcgatacgg tcgccaaggg atcgacctcg
     1081 cagttcgatg cgaccgcatt catctcgggt aacacgagca agttcttcaa cttgtggggc
     1141 ggcccggtcg ggttcgtgct cggtgccgag tatcgtaccg acaacgtcac ctataatctc
     1201 gatcgcgata cacagctggg atacacgttc tacaacgcta tcccatcgtt caccgcgcca
     1261 aagtccaagg tgaaggaagc gttcggcgag atcagcctgc cgatcttcaa ggaccgtccg
     1321 ttcctgcgcg agcttgaagt ggacgccgct gcccgtgtgt cggattacaa tctgggcacg
     1381 accggcacag tttgggccta caacgtcaac ggtatttact cgccgttcaa cgggctgcgt
     1441 ttccgcgcga actacgctcg tgcggtccgc gctcccaacc aggtcgaact gttctcgcca
     1501 tcaggtcaga actttgcgcc tgggttctcg gatccgtgct cggcgatcaa tatcaacaac
     1561 gggtcgcagt tccgcgaagc gaactgcaat gccgccgggc gtcctgccgg ttacgacttc
     1621 cgttactctt cgtcgctcgg cttcaagagc ggcggcaatc cgaatttgaa ggcagagact
     1681 tccgacagct acacggccgg tttcgtgatg acgccgcaat tcctgccagg gttcgagacg
     1741 tcggtcgatt attacaacat caagctgaag aactcgattt cgttcgtcgg cgcccaagcc
     1801 gtcgccaacg cttgctatga cgtgccaaca tcgccgaacg tattctgcag ccagttcgaa
     1861 cgtgctggac ccagtggcgg cccgcatggt gagcagccgt tccagatcat cgagggaagc
     1921 ctgctgtcag cgccgctgaa ctacgccaag ctgacggcca gtggtatcga tgtggaagtc
     1981 gcttatcggc gccaggtcgg tgatatcggc cggttggata cgcgcctgac ttatacgcac
     2041 gtgcttaacc ggtcgaatta catctcggcg accgatccca acttcgaaga ccagcaattg
     2101 ctggagttgg gtgacccgca ggatgcgttc aactggaata cgacgatcca gcatgggcgg
     2161 ttcacgttcg gttatcagat gcgctacgtc gggaagatgg tgctgaacct ttatgaggat
     2221 ttcttcagcg ttcagggccg tccgcctcag aaccccgact attccaaccg ccagttctac
     2281 ccggccaagt tctaccatga catacggctg ggcatcgacg tgacgcgcaa gtataatttc
     2341 tacctgggtg tcgataacct gacgaatacc attccgcctt acggcttgac gggtatcggc
     2401 ggaggaagcg cgatttacga caaccgcggt cgtttcatgt atgcgggcgt caaggcgaac
     2461 ttctaagacg tctaacttgc agaacctgga ggccgggcgt ttcgcccggc ctcttttttg
     2521 ttgttcgtgc ggccgtaacg ctgcacttcc tcaatatgat cgcgatcgac agcgcatttg
     2581 acctcgtgcg agccgggcgg gcttccgagg ccgtgttcga gctggagtct gcggcgagcg
     2641 ccggagatgc ctcaagcgcg ctcgagctcg gaatctggtt ccttgaaggg aaacatgtcc
     2701 cacgcgacct ccgccggtcg cgcgaagcat tcaggcgcgc cgccgagcta ggcaatgcta
     2761 gtgctgaaca ggttttcacc tcattcatgg cgatcggcgt cggcgcgagc gcagattggg
     2821 ccggagcgct gcaacgctta cgggcgtttg cccgggttga tagtgctgcg gcggagcagc
     2881 ttgcgttgct cgatcgcatg aatctgaccc aggcaggcga tcccggcgaa catccggttg
     2941 cacgacagct gagtgtcgag ccgcaggtca actgtttcga acaactcttt agcggcgacg
     3001 aatgctctta ccttatatcg cgcgcgcggc cgttgttgca gccctcggtg atcgtcgaac
     3061 cgtcaaccgg ccaattgcag ccgcatccaa tcagaacgtc cgaaggggcg atgttcccat
     3121 gggcatcgga ggatctggtc attcacgcgc tcaatcgccg cctggcgtgg gcaagcggca
     3181 ccgcagttgc gtgcggcgag ccggtccagg tcctgagcta cagaccgggc cagcaatatc
     3241 gaccgcacct cgacgccttg ccgaacacgg gcaaccagcg gatcctcacg atgttggttt
     3301 atttgaacga aggctattcc ggcggtgaga ctttcttcac tcgaaccgga ctgaagttcg
     3361 ccggcaagac gggcgacgga ctgctgttcc gaaatgccgg tgcaggaggg catcccgacc
     3421 acaacgcgga acatgccggc ctgccggttc ttcgcggcga gaagttcatt gccagtcgat
     3481 ggatccgcga acagccgctg atgcctcatt agacgcgttt tccccttcat ttcgacacac
     3541 atgcgcgcct atgacatcgg catgggtgcg gggggcattc acgcgtgaac ggccatccgg
     3601 ccgcaatcat cgccatggca gggctggcac tgctgtggct tgccgttggt ttcgtcatcg
     3661 cgatcatcgc ggcgcaacgc ttgaggcttg cccaacaggt gctcgatgcc gcgcgggcga
     3721 atgcgcgt
//
LOCUS       DATIMH010000372         3660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4709849, whole genome shotgun sequence.
ACCESSION   DATIMH010000372 DATIMH010000000
VERSION     DATIMH010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4709849"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VLI90_06465"
     CDS             complement(<1..1060)
                     /locus_tag="VLI90_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13884.1"
                     /translation="MIDKIFKAYDVRAIYPNPLNEENAWKVGHATAQFLKRSRDKLGA
                     DLKVKHENTMVVGRDMRPSSPDLARALMDGIRSTGMNVVDIGTVDTSFVYFAINELDT
                     VGGIMTTASHNPVQYNGFKISGPKAKPIGAATGLDDIKRIATGLRVGQTGLKGSIEER
                     DLWPQYKKKVLSFLDLKRPLKVAIDASNGMAGKMVPAVFGDVPNLTIVPILFEITGSF
                     VHEPNPLVESNLDMLKQKMTETPCDLGACFDGDADRCMFLDERRKYIGSDMITALLAK
                     DFLGRPENAGNTVVYDLRSSHVVPDEINAAGGVPRRDRVGHAFIKKTMAETKAIFGGE
                     LSGHIYFRDFYYADSAAVA"
     gene            complement(1190..2728)
                     /gene="metG"
                     /locus_tag="VLI90_06470"
     CDS             complement(1190..2728)
                     /gene="metG"
                     /locus_tag="VLI90_06470"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSV13885.1"
                     /translation="MSNRFYITTPIYYPNGEPHLGHVYTTLCADTIARYHRLAGDDTF
                     FLTGTDEHGLKMTKEAAEQGIEPSALADRYSSTFENLWKEFHVTNDDFIRTTQPRHKA
                     SVQRIVEQMLAKGDIYLGSYEGWYDVGQEEFVTETEAKAAEFKSKVSGKPLTRQSEKS
                     YFFRLSKYVDRVLQHIESHPQFIQPDSRRNEVVSKLKIGVADLSISRATLKWGVPMPN
                     DPEHVVYVWIDALSNYITALGYGTADDGRCKKYWPANVHLIGKEIMWFHTVYWPAMLF
                     SLELPLPTTVFAHGWWTSDGRKMSKSMGNFIDLAKLREVSAKYGLDALRYYLLRAAPF
                     GSDLDWSEKEFNAAYFELSKKLGNCLNRATNMTGRYRQNVVPAAGELQEIDRAVVAQI
                     EALPSKLADAYARYALQDCALLPIEVVRAIDGYIEATAPFKMAKDPAQSARLDTVLNM
                     LVRGMHAVLVSLLPILPEKAVDGLKQLGVDAQGRSLAELFAAAPSAGAKLGEGHALFP
                     AIES"
     gene            2799..3113
                     /locus_tag="VLI90_06475"
     CDS             2799..3113
                     /locus_tag="VLI90_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13886.1"
                     /translation="MSQMVHCDLCGITIAPGAHYVVRIDVFADPAMPKMSTDEIDQLT
                     SAEHLAELMQQLQQMSEDDMQDQVHRRFEYKLCQPCQQRFLANPLGRPRSNPMVRARG
                     NN"
     gene            3113..>3660
                     /locus_tag="VLI90_06480"
     CDS             3113..>3660
                     /locus_tag="VLI90_06480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSV13887.1"
                     /translation="MNRLPYLGATDWPGVLREIRGRAQRYDISGDWPTTDIQALDAAG
                     AMRWSVPKEFGGDELPPLELHLRYEELARSSLATALILTQRDSAVGLVTASIGAAKRT
                     TMLHRFAENKHFTTVGIAQLTTSRQGSMPALRARRVNGGYQLDGIIPWCTGAPMAQFI
                     VAGATLDDGQQILFLMEPHLSGV"
BASE COUNT          653 a         1191 c         1103 g          713 t
ORIGIN      
        1 acgcgacggc ggcggagtcg gcgtaataga aatcgcggaa gtagatatgg cccgacaatt
       61 cgccgccgaa gatggctttg gtttccgcca tcgtcttctt gatgaacgcg tgacccacgc
      121 ggtcgcgccg cggcacaccg ccggcggcgt tgatttcgtc cggcaccacg tggctcgagc
      181 gcaggtcgta aacgacggtg ttccccgcgt tctccggccg gccgagaaaa tccttcgcca
      241 gcaacgctgt aatcatgtcc gaaccgatgt acttccggcg ttcgtcgaga aacatgcagc
      301 gatcggcgtc gccgtcgaag cacgccccca ggtcacacgg cgtctcggtc atcttctgct
      361 tcagcatgtc caggttcgac tcgaccagcg ggttgggctc gtgcacgaac gagccggtga
      421 tctcgaacaa aatcggcacg atcgtcaggt ttggcacgtc accgaacacc gccggaacca
      481 tctttccggc catgccgttg ctggcgtcga tcgccacctt caacggccgc ttcaggtcca
      541 ggaagctcag caccttcttc ttgtattgcg gccacaggtc gcgctcttcg atgctgccct
      601 tcaacccggt ctgaccgacg cgcaggccgg tggcgatgcg cttgatgtca tcgagcccgg
      661 tcgccgcgcc gatcggtttc gctttgggtc cgctgatctt gaaaccgttg tactgaaccg
      721 gattgtgcga cgcggttgtc atgatcccgc cgaccgtgtc gagttcgttg atggcgaagt
      781 agacgaacga cgtatcgacc gtgccgatgt cgacgacgtt catgccggtg gaacggatgc
      841 cgtccatcag cgcgcgggcg aggtccggcg acgacggccg catgtcgcgg ccgacgacca
      901 tcgtgttctc gtgcttcacc ttcaggtcag cgcccagctt atcgcggctg cgcttgagga
      961 actgtgccgt cgcgtggccg actttccacg cgttttcttc gttcagcggg ttcggataga
     1021 tggcgcgaac gtcatacgct ttgaaaatct tgtcgatcat ggtgcggttg tcctgagaaa
     1081 aagagggcgc ggcgaagtgt attcgcgagc ggcgggtgtc gcaattcgcg ccacctgtag
     1141 ccgcgacgcc tgcgtcgcca ttttcgcaaa ggcgacgcag gcgtcgcagc tacgattcga
     1201 tcgccgggaa cagcgcatga ccttcgccaa gtttcgcgcc ggcgctaggc gctgccgcga
     1261 acaactccgc cagcgatcgt ccctgtgcat caacccccag ctgcttcaac ccatccaccg
     1321 ccttctccgg caggatcggc agcaggctga ccagcaccgc gtgcatgccg cggacgagca
     1381 tgttgagaac tgtgtcgagc cgtgctgatt gcgccggatc cttcgccatc ttgaagggcg
     1441 cggtcgcttc gatgtagccg tcgatcgcgc gcaccacctc gatcggcagc agcgcgcagt
     1501 cctgcagcgc gtatcgcgcg tacgcatccg ccaacttcga cggaagcgct tcgatctgcg
     1561 cgacaacagc gcgatcgatc tcctgcaact cccccgccgc cgggacgacg ttctgacgat
     1621 atcggcccgt catgttcgtc gcgcggttca ggcagttgcc cagcttcttc gacagctcga
     1681 agtacgcggc gttgaactcc ttctcgctcc aatccaagtc agacccgaac ggcgccgcgc
     1741 gcagcaggta atagcgcagc gcatcaagcc cgtacttggc cgacacctcg cgcagcttcg
     1801 ccaagtcgat gaaattcccc atcgacttgc tcatcttccg cccgtcgctg gtccaccagc
     1861 cgtgcgcgaa caccgtcgtt ggcagcggca gctcgaggct gaacagcatc gccggccaat
     1921 agaccgtgtg aaaccacatg atctccttgc cgatcaggtg cacgttcgcc ggccagtatt
     1981 ttttgcaccg gccgtcgtcg gccgtgccgt agccgagcgc cgtaatgtag ttgctcagcg
     2041 cgtcgatcca cacgtagacg acgtgctcgg gatcgttcgg catcggcacg ccccacttca
     2101 gcgtcgcccg gctgatcgac agatccgcca caccgatctt cagcttcgag acgacctcgt
     2161 tccgtcggct gtccggctgg ataaactgcg ggtgcgattc gatgtgctgc agcacgcgat
     2221 cgacatattt actgaggcgg aaaaagtagc tcttctccga ttgccgggtc aacggtttgc
     2281 cgctgacctt cgacttgaac tccgccgcct tggcttcggt ctcggtgacg aattcttcct
     2341 gccctacgtc gtaccagcct tcgtagctgc caagatagat gtcccccttg gcgagcatct
     2401 gctcgacgat ccgctgcacc gacgccttgt gccgcggctg ggttgtgcgg atgaagtcgt
     2461 cgttggtaac gtgaaactcc ttccacaagt tttcgaacgt cgacgaataa cgatccgcca
     2521 gcgcgctcgg ctcgatgccc tgctcggccg cctccttggt catcttcagc ccgtgttcat
     2581 cggtgccggt gaggaagaac gtgtcgtccc cagcgagccg gtgatagcgc gcgatcgtgt
     2641 cggcgcacag cgtcgtgtaa acgtgcccca ggtgcggctc gccgttgggg taataaatcg
     2701 gcgtcgtgat gtagaagcga ttgctcatcg ttaatctcgc ggacaacctt aaccaacagc
     2761 cctgccgtcg gcactattcc agcgcataca atagcagcat gtcccagatg gttcactgtg
     2821 acctgtgcgg catcaccatc gcgcccggcg cgcattacgt ggttcgcatc gacgtcttcg
     2881 ccgacccggc gatgccaaag atgtccaccg acgaaattga ccagctcacg tcggccgaac
     2941 atcttgccga gttgatgcag caactgcagc agatgagcga ggacgatatg caggaccagg
     3001 tgcaccggcg gtttgaatac aagctgtgcc agccgtgcca gcagcgattt ttggccaacc
     3061 cactgggccg gccgcggagc aatcccatgg ttcgcgcgcg ggggaacaac tgatgaaccg
     3121 cctgccttac ctcggtgcca ccgactggcc cggcgttttg cgtgaaatcc gcggccgggc
     3181 gcagcgctac gacatcagcg gcgattggcc gacgacggac atccaggcgc tcgacgccgc
     3241 gggggccatg cggtggtcgg tgccgaagga attcggcggc gacgagctgc cgccgctgga
     3301 acttcacctg cgatacgagg agctggccag atcatcgctg gcgacggcgc tgatcctgac
     3361 gcagcgcgac tcggcggtcg ggctggtcac cgcgtccatc ggcgcagcga agcgaacaac
     3421 aatgctgcat cgcttcgcgg agaacaagca tttcaccacg gtcggcatcg cgcagctgac
     3481 gacgagccgg cagggctcga tgccggcgct tcgcgcgcgg cgcgtcaacg gcggctatca
     3541 gctcgacggc atcatcccgt ggtgcaccgg cgcgccgatg gcgcagttca tcgtcgccgg
     3601 ggcgacgctg gacgacgggc aacagatcct gttcctgatg gaaccgcacc tgagcggcgt
//
LOCUS       DATIMH010000373         6947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1821345, whole genome shotgun sequence.
ACCESSION   DATIMH010000373 DATIMH010000000
VERSION     DATIMH010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821345"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VLI90_06485"
     CDS             <1..440
                     /locus_tag="VLI90_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13888.1"
                     /translation="PPSVEINFQLNNDGSQTVTFDPHTIQLSDAGLTRFDPPIVRPEQ
                     PPITLDPAQSTTITAYFPFPPGRTADNMEMSSLQLRWNVHIGDRSVSQTVFFRQSSPT
                     YYYYRPYYYYDDPYWYGGPYWYGPSVFVGPRVFIGPRFHHRRW"
     gene            complement(449..1480)
                     /locus_tag="VLI90_06490"
     CDS             complement(449..1480)
                     /locus_tag="VLI90_06490"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HSV13889.1"
                     /translation="MACVGAAPAFQEAIAPRAWSFPRDHGRHDGFKLEWWYYTGHLRD
                     ASGHRFGYQLTFFRSAFRAQPIERQSPWAMTNLYFAHAAISDIDAKSFIFKDRLQRAR
                     AGLAYASDQTLDVSLLDWSAKLEGDQTHLHAADDGLSIDLTCGPGRGPVLEGPGGVNA
                     KGREVGQASYYYSMTRLPTSGTLSIHNQTYQVEGQSWMDHEFSSNALGGSQIGWDWMG
                     LHLSSGEDLMLYRLRNKSGGVDFLSGTRITPDGQPHYLSDRDLTMEPSEDWKSPTSGA
                     SYPQRWLIQCAGLPPLIVHSQMPGQELVTSGSSDVTYFEGAAEVLNADNNSPAGEGYL
                     EMTGYAPAK"
     gene            complement(1588..4215)
                     /locus_tag="VLI90_06495"
     CDS             complement(1588..4215)
                     /locus_tag="VLI90_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV13890.1"
                     /translation="MIQFFAKLTHGHFRRHRLEALLCLLGVALGVAVVIAIDSAVAAC
                     VRSFSGAVSTLAERSTHSIFAEGGTIADQAYMDLLKRKLPYPLAPVIDRGVLIDRPSP
                     TPVSEGTPEDPGSAARKDPGLRSTSDTGVGGSSTFARLIGVDVFSERALRSFTKMQST
                     LDDAAFRTFLTEPGQVVLVDALAKRLNVAPGSTLRITSGARRSLVHVVGVVQPTGIAR
                     SQINDLVIADLATAQELTGLIGQIDRIDTVLDNDQQVSALTSQLPSGLILRSTRQQSQ
                     SLAQLIQSYRLNLNALSLMASFVAVFIVYNSMLISVQQRARSLGILRCLGAARPQLAG
                     LYASEAVAFALLGGAIGVPGGWLLARILVGYVSTTINDLYAAINPGPVQLDAAMWAKG
                     LAVSLCSCIVGALVPLVQASRMPPINAFRASEHQRASRWMPVRLLAIGAILLAITYGV
                     YLIPTDSPLVGFVMALLAAFGFALVCPWFTRIGCRAIDAAARPAQLLPLQMATAGVAR
                     SLGITGVAVAATMLAMAMNVGIRTMVSSFRGSLSDWFGHRFAADIFVGPELLLNHHVD
                     ATIDPRVEQWVRAQPEAKSVVTNRSREVPLGGKSTLLFATDVADTLKTLPMKSVDRGR
                     PFDPKTDALISEPLAGRIKRSTGDTFTVSAPTGPQTFRVYGVFYDFGTERGQFMIDRR
                     TYAAAWRDDALTSLHVTIAPGVDRTALAGRWSAELRQQFPVVVNSFDSVKTEAMTVFD
                     RTFKVTEVLTWLGGGVAFCGLAGSLLALALARQRDYSILAAVGMSGRQTAAWVLGQGI
                     VIAWASALIAPVAGTVLAYILAYVIQYRSFGWSIPTHAQPMFWVQNFLIATSAALVAA
                     VYPIYRLRAAPPAASLRPE"
     gene            complement(4275..5003)
                     /locus_tag="VLI90_06500"
     CDS             complement(4275..5003)
                     /locus_tag="VLI90_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV13891.1"
                     /translation="MDTISPTEPETRRATQEQIGNMAEVDRVQKTYGSGDSGFTALHE
                     VSLRVPRGRSIALLGRSGSGKSTLLNLLGAVDRPTAGTIRIGGTDLSTLSDAELALFR
                     RRRLGYVFQSFHLVPSLTVFDNVAVPWTLDRALTSERKGQVTALLQRLGVAEKAKRYP
                     DELSGGQQQRVALARAIIHRPDLLLADEPTGNLDAHTGRVILDLLDELRREFGLTVVM
                     ATHSPEAAERCDERVMLSDGRIEK"
     gene            5223..5891
                     /locus_tag="VLI90_06505"
     CDS             5223..5891
                     /locus_tag="VLI90_06505"
                     /inference="COORDINATES: protein motif:HMM:NF021141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HSV13892.1"
                     /translation="MGFTDMDGSIFRARPARTAGLIVALYFMLTAATGCSTTVQPPAR
                     PLQPVTIYLTDEFIHSSVVMPVDDGRYVEYAFGDWTYAALNRHDPFHTVRALFFSPQG
                     ALGRRYLHVDPASKDLAPELKGITVNKVVVDREQVRALEAKLDGRFVPGRSQAENPEN
                     HFWWVTVDDSYALWSNCNTLTKRNLRALGCEVESRSLFAMYDVKAPAPPLVYASTGAR
                     EGSK"
     gene            complement(5931..>6947)
                     /gene="dusB"
                     /locus_tag="VLI90_06510"
     CDS             complement(5931..>6947)
                     /gene="dusB"
                     /locus_tag="VLI90_06510"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HSV13893.1"
                     /translation="IGSVQLATNLLLAPIAGYCDLAFRLAARSCGGVGMACTDLLCPE
                     GVLRENRRSMELAITCEADSPLCMQLYGGNVELLCEAARWAEDRGAHVIDINMGCPVD
                     KITKRDGGSKLLCDPDNTMRMVERIVRVLRHTPLTAKLRLGWDDTCIVAPQLARRLEN
                     AGVQMITIHGRTTEMKFSGEARLDGIAEVVAAVDRIPVVGNGDVRTPQDAKRMIDVTG
                     CAGVMIGRGALSMPWIFRDTWSYLTTGEIPEPPTIQEKCQLMRDHFHHLARFRNERSA
                     VIEFRKRVSWYAKQMHPCEMLRDEMRTINSTADFESILSSFLEWRTMRDEMTQEPAAC
                     GFAN"
BASE COUNT         1214 a         2218 c         2295 g         1220 t
ORIGIN      
        1 ttccgccttc agtcgagatc aacttccagt tgaacaacga tggctcgcag accgtcacgt
       61 tcgacccgca cacgattcag ctgagcgacg cggggctgac gcgatttgat ccgccgatcg
      121 ttcgcccgga acagccaccg atcacgctgg accctgcgca gtcgacgacg atcaccgcgt
      181 acttcccgtt cccgcccggg cggaccgccg acaacatgga gatgtcttcc ctgcaattgc
      241 ggtggaacgt gcacatcggc gaccgcagcg tgtcccagac cgtgttcttc cggcagtcat
      301 cgccgacgta ttactactat cggccctatt actattacga cgacccctac tggtacggcg
      361 ggccgtactg gtatggcccg agcgttttcg tcgggccgcg cgtattcatc ggaccgagat
      421 ttcatcatcg ccgctggtaa gtgccggcct atttcgcggg ggcgtaaccg gtcatctcca
      481 gataaccttc gccagcgggt gagttgttgt cggcattcag cacctccgcc gcgccttcga
      541 aataggtaac gtcgctcgat ccggacgtca ccagctcctg ccccggcatc tggctgtgca
      601 cgatcagcgg cggcaatccg gcgcattgga tcagccagcg ctgcggatag ctcgcgccgc
      661 tggtcggact cttccaatct tccgacggct ccatcgtcag gtcgcgatca gacagataat
      721 gcggctgacc atcgggcgtg atgcgtgtgc cggagagaaa atcgacgccg ccggatttgt
      781 tgcgcaggcg atagagcatc aggtcttcgc cgctgctcag atgcaggccc atccagtccc
      841 agccgatctg gctgccgccc agcgcgttgc tggagaactc atggtccatc cagctttggc
      901 cttcgacttg ataggtttga ttgtgaatcg aaagcgtgcc ggacgtcggc agccgtgtca
      961 tcgagtaata gtacgacgcc tggccaacct cgcggccctt ggcgttcaca ccgccggggc
     1021 cctcgagaac gggaccgcgg cccgggccgc aggtgaggtc gatggagagc ccatcgtcgg
     1081 cggcgtgcag gtgcgtttgg tccccttcta gctttgcaga ccagtcgagc aacgagacgt
     1141 cgagcgtctg atcggatgcg tacgccaagc cagcccgggc gcgctgcagg cggtctttga
     1201 agatgaacga ctttgcatcg atgtccgaaa tcgccgcgtg ggcgaagtac aggttcgtca
     1261 tcgcccaggg actctggcgt tcgatcggct gcgcgcgaaa ggcgctgcgg aagaacgtca
     1321 gctggtaacc gaagcgatgc ccgctcgcat cgcgcaggtg gccggtgtag taccaccatt
     1381 cgagcttgaa cccatcgtga cggccatgat cgcgcgggaa ggaccaggcg cgcggcgcga
     1441 tcgcttcctg aaatgccggc gcggcgccga cgcaggccat caccaacaaa agcaccgccg
     1501 ccgcttgcgg cttcgcaaac caaaccgcgc cacatggtct gctaacccgc aagcggcggc
     1561 gcttttgcaa atgaagcttg gccatcatca ctccggtcgc aaactcgccg cggggggcgc
     1621 tgcgcgcaat cgataaatcg gatacaccgc cgcgaccagc gcagcgctcg tcgcgatcaa
     1681 aaagttctgc acccagaaca tcggctgcgc gtgcgtgggg atgctccagc cgaacgagcg
     1741 gtactggatc acgtacgcca ggatgtacgc cagcacggtg ccggcgaccg gcgcgatcag
     1801 cgccgacgcc catgcgatca cgataccctg ccccaacacc cacgcggcgg tctgccggcc
     1861 gctcatgccg acggcggcga ggatgctgta gtcgcgctgt cttgccagcg ccagcgcgag
     1921 caacgaccca gccagcccgc agaacgcgac gccgccgccg agccaggtca gcacctccgt
     1981 caccttgaac gtgcgatcga acaccgtcat cgcttcggtc ttcaccgaat caaagctgtt
     2041 gacgacgacc gggaactgct ggcgcagctc ggccgaccat cgcccggcca gtgcggtgcg
     2101 atcgacgccg ggcgcgatcg tcacgtgcag cgatgtcagc gcatcatcgc gccacgcagc
     2161 cgcgtacgtg cggcggtcga tcatgaactg cccgcgctcg gtgccgaagt cgtagaagac
     2221 gccatagacg cggaacgttt gcggtcccgt cggcgcgctg acggtgaatg tgtcgccggt
     2281 cgatcgcttg atccgcccag ccagcggctc gctgatcagc gcatcggtct tggggtcgaa
     2341 tggccggccg cgatcgacgc tcttcatcgg cagcgtcttg agtgtgtcgg caacatccgt
     2401 cgcaaagagc agcgtcgatt tcccgcccag cggcacctcg cgcgaccgat tcgtcacgac
     2461 ggacttcgcc tcgggttgag cgcggaccca ttgctcgacg cgcgggtcga tcgtcgcatc
     2521 gacgtgatgg ttcagcagca gctcggggcc gacgaagatg tcggcggcga agcggtggcc
     2581 gaaccagtcg gacaaggacc cgcggaacga gctgaccatc gtgcggatgc cgacgttcat
     2641 cgccatcgcc agcatcgtcg ccgccacggc gacaccggtg atgccgagcg atcgcgccac
     2701 ccccgcggtg gccatctgca gcgggagcag ctgcgccggt cgcgccgcgg cgtcgatcgc
     2761 gcggcaaccg atgcgcgtga accacgggca aaccagcgcg aaaccgaacg ccgcgaggag
     2821 ggccatcacg aaaccgacca gcggtgagtc ggtggggatg agataaacgc cataagtgat
     2881 cgccagcagt atcgcgccga tcgcgagcag gcgaaccggc atccagcgcg acgcccgctg
     2941 gtgctctgaa gcgcgaaacg cattgatcgg cggcatgcgc gacgcctgca ccagcggcac
     3001 cagcgcgccg acgatgcagc tgcacagcga cacggccaaa cccttcgccc acatcgccgc
     3061 gtccagctgc accgggccgg gattaatggc ggcgtacaga tcgttgatcg tcgtcgagac
     3121 gtagccgacg aggatccgcg ccagcaacca tccaccgggc acaccgatcg ccccgccgag
     3181 tagcgcgaag gcgaccgctt ccgacgcata cagccccgcc agttgcgggc gcgctgcgcc
     3241 gaggcatcgc aggatgccca ggctgcgggc gcgctgctgg accgaaatca acatgctgtt
     3301 gtagacgatg aagaccgcga cgaagctggc catcagcgac agcgcgttga ggttcagccg
     3361 gtatgactgg atcagctgag cgagcgactg cgactgctgg cgcgtcgaac gcaggatgag
     3421 ccccgacggc agttggctcg ttagcgcgct gacctgctgg tcgttgtcga gcaccgtgtc
     3481 gatgcggtcg atctggccga tcaggcccgt cagttcctgc gccgtcgcga ggtccgcgat
     3541 gacgaggtcg ttgatctgcg accgcgcgat gccggtgggt tggacgacgc cgacgacgtg
     3601 aacgaggctg cggcgtgcgc cgctggtgat gcggagcgtc gagccgggcg cgacgttcag
     3661 tctcttcgcc agcgcatcga cgaggacgac ctgcccaggc tcggttagaa acgttcggaa
     3721 cgcggcgtcg tcgagcgtgg attgcatctt cgtgaacgag cgcagcgcgc gctcggagaa
     3781 gacgtcgaca ccgatcagcc gcgcgaaagt agaagacccg ccgacgccag tgtctgaggt
     3841 actccgaaga cccggatcct tccgcgccgc agaacccggg tcttcgggag taccctcaga
     3901 caccggcgtc ggggatggtc gatcgatcaa caccccacga tcgatcaccg gtgccagcgg
     3961 gtacgggagc ttccgcttca gcagatccat gtacgcctga tccgcgatcg tcccgccttc
     4021 ggcgaagatg ctgtgcgtcg atcgctcggc cagcgtgctc acggctccgc taaagctgcg
     4081 cacgcacgcg gcgaccgcgg agtcgatcgc gatcacgacg gcgacgccga gcgcgacacc
     4141 caggaggcac agcagcgctt cgagccggtg ccgtcggaag tggccgtgcg tgagcttggc
     4201 gaagaattgg atcatctgtg gcacgggcgt ctcgcctgtg tcgaacggtt ccagacacgg
     4261 gcgagacgcc cgtgctactt ttcgattcga ccgtcgctca gcatcacgcg ctcgtcgcag
     4321 cgctcggcgg cttcgggcga gtgcgtggcc atcaccaccg tcagcccgaa ctcccgccgc
     4381 agttcgtcga gcagatcgag gatcacccgg ccggtgtggg cgtcgaggtt tcccgttggt
     4441 tcatccgcca gcaacaggtc cgggcggtgg atgatcgcgc gagccagcgc gacgcgctgc
     4501 tgctgcccgc cggaaagttc atcggggtaa cgcttggcct tctcggctac gccgaggcgt
     4561 tgcagcagcg cggtcacctg ccccttgcgc tcgctcgtca gcgcgcgatc cagcgtccac
     4621 ggcacggcga cgttgtcgaa gactgtcagg ctcggcacca ggtggaacga ttggaagacg
     4681 tatccgagtc gccggcggcg aaagagggcc agttcggcat cgcttagcgt cgatagatcg
     4741 gtcccgccga tgcgaatcgt cccggcggtg ggacgatcga cggcgcccag aagattgagc
     4801 agcgtgcttt taccagaacc gctgcggccc agcagtgcga tcgaccgccc gcgcggcacg
     4861 cgcagcgaca cttcgtgaag tgcggtgaac ccgctgtcgc cgctgccgta cgtcttttgc
     4921 acgcgatcga cctcggccat attgccgatt tgttcttgcg tcgcgcggcg cgtctccggc
     4981 tcggtcgggg aaatcgtgtc catcgtggtg gcgacattgt aggcgatgcg cggccgattc
     5041 ggtcgcggcg gctttcaatt atggcgattg cttgtgaagc gcgggcgcgc cctggtcccc
     5101 tctcccggcg taccgggcga gggggaaaga gcgctgcgct tttcgtcgcg gcggggtcat
     5161 ggtatttttt acggtctctt cacgtgaccg tttgggtgca ggggtttttc atgggcaggc
     5221 gaatgggatt cacagacatg gatggttcga tctttcgcgc tcgcccggca cggacggccg
     5281 gtttgatcgt cgcgttgtat ttcatgctga cggcggcgac cggatgcagc accacggtgc
     5341 agcccccggc gcgaccgctg cagccggtga cgatttacct cactgatgag ttcatccact
     5401 ccagcgtggt gatgccggtg gatgatgggc gatacgtgga atacgccttt ggtgactgga
     5461 cgtacgcagc gctcaaccgg cacgatccgt ttcacacggt tcgcgcgctg ttcttttcgc
     5521 cgcagggggc gctggggcgg cggtatctgc acgtcgatcc ggcgtcgaag gacttggcgc
     5581 cggagctgaa ggggatcacg gtcaacaagg tggtggtcga tcgcgagcag gtgcgggcgc
     5641 tggaggcgaa gctggatggc cgtttcgtgc cgggccgatc gcaggcggag aatccggaaa
     5701 accatttctg gtgggtgacg gtggatgatt cgtatgcgct gtggagcaac tgcaatacac
     5761 tgacgaagcg aaacctgcgg gcgctggggt gcgaggtgga gagccggtcg ctgtttgcga
     5821 tgtatgatgt gaaggcgccg gcgccgccgc tggtgtatgc gagcacgggg gcgcgggagg
     5881 ggtctaagta gttgccgccg ctcgcgggct cgcaagcgat gccgcgcggt ttagtttgcg
     5941 aagccgcaag cggcgggttc ttgtgtcatt tcatcgcgca tcgtccgcca ttccaggaag
     6001 ctcgacagga tcgactcgaa atccgccgtt gaattgatcg tgcgcatctc gtcgcgcagc
     6061 atctcgcagg ggtgcatttg cttggcgtac caactcaccc gcttgcggaa ttcgatgacg
     6121 gcggagcgct cgttgcggaa gcgggccagg tggtggaaat ggtcgcgcat cagctggcac
     6181 ttctcttgga tcgtcggcgg ctcggggatt tcgccggtcg ttagataact ccacgtatcg
     6241 cggaagatcc acggcatcga tagcgccccg cggccgatca tcacgccggc gcagccggtg
     6301 acgtcgatca tgcgcttggc gtcctgcggc gtgcgcacgt cgccgttgcc gacgacggga
     6361 atgcgatcca ccgcggcgac gacttcggcg atgccgtcca gccgcgcttc gccggagaac
     6421 ttcatttccg ttgtccgccc gtgaatcgtg atcatctgca cgccggcgtt ttccaaccgg
     6481 cgggcgagct gcggcgcgac gatgcaggtg tcgtcccagc cgaggcgaag tttcgccgtc
     6541 aacggcgtgt ggcgcagcac gcgcacgatg cgctcgacca tgcgcatcgt gttgtccgga
     6601 tcgcacagca gcttgctgcc gccgtctcgc ttggtgatct tgtccaccgg acaacccatg
     6661 ttgatatcga tcacatgggc gccgcggtct tccgcccatc gcgccgcttc gcagagcagc
     6721 tcgacattgc cgccgtatag ctgcatgcac agcggagaat cggcttcgca ggtgatcgcc
     6781 aattccatcg atcggcgatt ttcgcgcagc acgccttcgg ggcagagcag gtcggtgcac
     6841 gccataccga caccgccgca gctgcgcgcg gcgaggcgga aggcgagatc gcagtaaccg
     6901 gcgatcggcg cgaggaggag gttcgtcgcg agttggacgg aaccaat
//
LOCUS       DATIMH010000374         4775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1821944, whole genome shotgun sequence.
ACCESSION   DATIMH010000374 DATIMH010000000
VERSION     DATIMH010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821944"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..884)
                     /locus_tag="VLI90_06515"
     CDS             complement(75..884)
                     /locus_tag="VLI90_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV13894.1"
                     /translation="MTNPTTERAAWDHTTHQAFYDYYADKSASALAQQSAERVKVIML
                     RLARRASMPSRLDVADIGCGPGSQAVLWAADGHRVCGLDVNKPLIDLARERAANAGLS
                     IRFEVGTATQLPWANETVDVCLLPELLEHVPDWRTCLREAARVLRSGGLFYVSTTNVL
                     CPRQQEFNLPLYSWYPSALKRYCERLAVTSRPSIANYARYPAVNWFSYYALRDFLAPL
                     GFRCLDRFDIVDEAAKTPAQARVVRAIRKSALLRFAAHVATPSLALVAVKS"
     gene            complement(961..2013)
                     /locus_tag="VLI90_06520"
     CDS             complement(961..2013)
                     /locus_tag="VLI90_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13895.1"
                     /translation="MAALSNSLAAALLVSSVAAAPGASPPATSASDFEVRKSAPGVVR
                     WFDFDSDAQLGSKAYGSNFGVLKGDKTSPVIDKTVKASGAGSLRFDLESYTASNGAGA
                     WYANFSKDLSVQFGENSEFYLQWRQRFNRAFVDTYFTEDWGDGKIHPQGGTKQIIVTT
                     GDQPKRIFNSCEAIEVVVTTYYQQRLPIAYNSCTGSASHPAYSPFYEKLPAADFRLQN
                     GTAPYCLYSNSGDDKTGVGPGCFGWVPDEWMTFQIGVTLGPRDNSKGEFLESRVRLWG
                     AREGQRSVLLVDWKPGIPGYFPLTAGPATDNQRFGKVWLLPYMTNKNHKQAHPLAQTW
                     YDDLIISTQRIADPAR"
     gene            complement(2113..>4775)
                     /locus_tag="VLI90_06525"
     CDS             complement(2113..>4775)
                     /locus_tag="VLI90_06525"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSV13896.1"
                     /translation="SDNVFKIQMPDGTTGVCPPKTAPVYRVFNNRADANHRYTMDTSV
                     RAAMIQKGAIAEGAGADGVAFCADASIDATSALTAMSVSIVSSTIAADTFAFSSSVGT
                     SVASAVLAYSWDFGDGVTQTGATASHRFVAAGTYNVILTVSDNDGHVASATKLITVAL
                     PTDNGSSAPPQLPLVAPIGSGDFDARKSAPGVVRWFDFDSAASLGPNQSSGPTNFGWF
                     PGRGTNAANPVLDTTVKASGAGSLRFDLNSQTQSDGAGMWFANFSPDLRTQFGANSEF
                     YVQMRVRWNQAFHDTVFLDAQGSTQGGIKFFDVGAGDQLGHQPTDEGSQGYYADGTPV
                     TMYFTNTDLEQVVNTYYQTRIVQMYAYSSAGMFAPMNEYVGSGDYKLQPAMPDCLYSK
                     AQTTGDVPGCFNFTANEWITLQLGITLGPKDVGGYHYTASRVRLWAAHEGQPSVLIND
                     WRPTHDDGTPVGGYFPPYAGNPAYDKRYGKVNIFPYLTNKNATQAHATGQMWVDELIV
                     STKRIADPAAVAVPNAKVSLGPNTAINLGPYAPAVPNGENPGNSQEATAYSGMQYDAH
                     RRQMVFFGGGHAASNSDTIMRLPLDTLTWTAEYSPTPKNAWTFDNYDFTLGAWKSGPG
                     GPYPRPAARHTLDELAVVGDELIVLAKVEGNYANLLGDWSAAPGASQGADHYTNTIGR
                     IAHYNFINKSWTFAPVSMPDTNFPAVSYDPPSGKVLILGRNGLFVYDPVGKTMARALD
                     LLDNVVIHDENGTPVDASALNINQNLVYFPLNGKHYYLGNLGAVFEVTLNRTDVSKST
                     ITKLTTTGTPPSGPEVGAAYDSVNNLIGVGPVNNVFYTFNPATKVWTSRTIEGGSPGS
                     VQYHCIDYDPVNNAYIFINTSFDTWAYRYQ"
BASE COUNT          895 a         1430 c         1496 g          954 t
ORIGIN      
        1 gcgtgaggag gacgtctctc actgcatgcc tcgacgcgcc atgtcgcctg gccgcacgac
       61 cgtcggtggc gatgtcatga tttcaccgcc acaagcgcca ggctcggggt cgcgacatga
      121 gccgcaaagc gaagcagtgc cgacttccgg atggcgcgga cgacgcgggc ttgcgcgggc
      181 gttttcgctg cttcatcgac gatgtcgaag cgatccaaac atcgaaatcc gagcggggcg
      241 agaaaatcac gcagcgcgta atagctgaac cagttgacgg cggggtaacg cgcatagttg
      301 gcgatcgacg gccgcgacgt caccgccagc cgctcgcagt agcgcttcaa agccgagggg
      361 taccaactgt agagcggaag gttgaattcc tgctgcctgg gacacaggac atttgtcgtg
      421 ctcacataga aaaggccccc gcttcgcagc acgcgggccg cttcccgcaa gcatgtgcgc
      481 cagtcgggca cgtgctcgag caattcgggc agcaggcaga cgtccaccgt ttcgttcgcc
      541 catggaagct gcgttgcggt tcccacctcg aaccggattg aaagaccggc gttcgccgcc
      601 cgctcgcgcg cgaggtcgat caacggcttg ttgacatcga ggccgcacac gcgatgtccg
      661 tccgccgccc atagcaccgc ttgcgatccc gggccgcacc cgatgtcggc aacgtccaac
      721 ctggaaggca tcgacgctcg ccgcgcgagg cggagcatga tgaccttcac gcgctcagcg
      781 ctttgctgcg ccaacgcgct cgcgctcttg tcggcgtaat agtcgtagaa cgcctgatgc
      841 gtcgtatggt cccaggccgc gcgttcggtc gtcggattcg tcatggatag gcgagtgccg
      901 gaacgcgacg cccgcgtcag cggtccgctt cccggttgcc ggcgatcgag gacgagcgtc
      961 tcatcgggcc ggatcggcga tcctctgcgt ggaaatgatc aggtcgtcgt accacgtctg
     1021 cgccagcggg tgcgcctgct tgtggttctt gttcgtcatg taggggagca gccagacctt
     1081 gccgaagcgt tgattgtcgg tggccgggcc cgccgttagc ggaaaatatc ccggaatgcc
     1141 gggtttccag tcaaccagaa ggaccgaccg ctgcccctcg cgcgcgcccc acaaacggac
     1201 tcggctttcg agaaattcgc ctttggaatt gtcgcgcggc ccgagcgtaa cgccgatctg
     1261 gaacgtcatc cattcatcgg gcacccagcc gaaacaaccc ggacccactc ctgttttgtc
     1321 gtccccgctg ttcgagtaca gacaatacgg cgccgttccg ttctgcaagc gaaaatcggc
     1381 ggccggcagc ttttcgtaga acggtgagta agcggggtgc gatgcactgc cagtacaaga
     1441 gttgtaagcg atcggaagcc gctgctggta gtacgtcgtg acgaccacct caatggcctc
     1501 gcacgagtta aagatccgtt ttggctggtc gccggtggtg acgatgatct gcttcgtgcc
     1561 gccctgcggg tgtatcttcc cgtcgcccca gtcctcggtg aaatacgtat cgacgaacgc
     1621 gcggttgaac cgttgtcgcc actgcaggta gaactcggag ttctccccga attggaccga
     1681 gaggtccttg gaaaaattcg cgtaccaagc gcccgcaccg ttcgacgcgg tgtatgactc
     1741 gaggtcaaaa cggagcgacc ctgcgcctga ggccttcacc gttttgtcga tgacgggtga
     1801 cgtcttgtcg cctttgagca caccaaagtt ggaaccgtac gctttggatc ccagttgcgc
     1861 gtcggagtcg aagtcgaacc agcggacgac gccaggcgca gatttccgga cttcgaagtc
     1921 ggatgcactc gtcgcgggcg gagaggcgcc gggcgcggct gcgacactcg acacgagcag
     1981 cgccgccgcc aagctattgc tcaatgcggc catgttcgag tgttcgcggg tcttcgcgag
     2041 tggaacggta agaagtattt ccctagtata acgtgccgtt cgtcgcgcct gtgcggcggt
     2101 ttgcgggcgc tgctactggt agcggtacgc ccaagtgtcg aagctggtat tgataaagat
     2161 atacgcgttg ttcaccgggt cgtagtcgat gcaatggtac tgaaccgatc cgggcgaacc
     2221 gccctcgatc gtcctgctcg tccacacctt cgttgccgga ttgaatgtgt agaacacgtt
     2281 gttcaccggg ccgacgccga tcagattgtt gaccgagtcg taagcagcgc cgacctcggg
     2341 tccggatggt ggcgtccccg ttgtcgtgag tttggttatc gtgctcttgc tgacgtcggt
     2401 cctgttcagc gttacctcga acaccgcgcc gaggttgccg aggtagtaat gcttgccatt
     2461 gagagggaag tagaccaggt tctggttgat gttgagcgcg ctcgcgtcga ccggcgtgcc
     2521 gttctcgtca tggatcacca cgttgtcgag caggtcgagc gctcgcgcca ttgtcttccc
     2581 gaccgggtcg tagacgaaaa gaccgttgcg tccgaggatc aacaccttgc ccgatggcgg
     2641 atcataggaa accgcgggga aattggtgtc cggcatggag acgggagcga aggtccacga
     2701 cttgttgatg aagttgtaat gtgcaatgcg gccgatagta ttcgtatagt gatcggcgcc
     2761 ctgcgatgct cccggcgctg cggaccagtc gccgagcagg ttggcgtaat tgccttccac
     2821 cttggccaag acgatcagct cgtcgccaac cacggcgagc tcgtcgaggg tgtgccgggc
     2881 cgccggacgc ggataggggc cgcccgggcc tgatttccat gcgccgagtg tgaagtcgta
     2941 attatcgaac gtccatgcat ttttcggcgt tggtgaatac tcggcggtcc acgtcaacgt
     3001 gtcgagcggc aagcgcatga tcgtgtcgct gttgctggcg gcatgaccgc ccccgaaaaa
     3061 caccatctgc cggcgatgcg catcgtattg catgccagag tacgcggtcg cctcttgcga
     3121 attgccagga ttctccccgt tgggtacggc aggagcatac gggcccaggt tgattgccgt
     3181 gttcgggccg agcgacactt tcgcattcgg gactgcgacc gcggcgggat cagcgatacg
     3241 cttggtggag acaatcagct cgtccaccca catctggccg gtcgcatgtg cctgcgtagc
     3301 attcttgttc gtcaggtacg gaaaaatatt caccttgcca tagcgcttgt cgtacgcggg
     3361 atttcccgca tacgggggga aatagccgcc gacgggcgta ccgtcatcat gggtcggccg
     3421 ccagtcgttg atcagcaccg acggctgtcc ttcgtgcgcg gcccacaaac gcacccgact
     3481 tgcggtgtag tgatagccgc ccacgtcctt cggacccagc gtgatcccga gctgcagcgt
     3541 gatccactcg ttggcggtga aattgaagca gccgggcaca tcgcccgtgg tctgtgcctt
     3601 ggagtacagg cagtcgggca tggctggttg gagcttgtaa tcgccgctgc cgacatattc
     3661 gttcatcggc gcgaacatgc ccgctgagct gtacgcatac atctgcacaa tgcgcgtctg
     3721 gtagtaggtg ttgacgacct gctcaagatc ggtgttggtg aagtacattg tcaccggcgt
     3781 gccgtcggcg taatagccct gggaaccctc atcggtgggc tgatggccca actgatctcc
     3841 ggcgccgacg tcgaagaact tgatgccacc ctgcgtcgac ccttgtgcgt cgaggaacac
     3901 cgtatcatga aaagcctgat tccagcgcac gcgcatctgc acgtagaact cggagttcgc
     3961 accgaattga gtacgcagat ccggcgaaaa gtttgcgaac cacatgccgg cgccatcgga
     4021 ttgcgtctgc gaattcaagt cgaagcgcag cgagccggca cccgacgcct tcaccgtcgt
     4081 atcgaggacg ggattggcgg cgttcgtccc acggcccggg aaccagccga aattcgtagg
     4141 tccactggac tgattcggtc ccagggaggc agccgagtcg aagtcgaacc agcgcacgac
     4201 gccgggcgcc gacttgcggg cgtcgaagtc tccactgccg atgggcgcaa ccagtggtag
     4261 ctgcggcggg gccgacgacc cgttatctgt tggaagggcg accgtaatca gcttcgtcgc
     4321 ggatgccacg tgaccgtcgt tgtcgcttac cgtcagaata acgttgtaag tcccggcagc
     4381 cacgaagcgg tgcgacgccg tcgcacccgt ctgggtcacg ccgtcaccga aatcccaaga
     4441 gtaagcgagc acggcagacg cgaccgatgt ccccacgctg ctactgaagg cgaacgtatc
     4501 cgcggcgatg gtgctcgaaa caatgctgac cgacattgcg gtaagcgccg aggtcgcgtc
     4561 gatcgacgca tcggcacaaa aagctacgcc gtcggcaccc gcgccttcgg caatcgctcc
     4621 cttctggatc atggcggcac ggacgcttgt gtccatcgta taacgatggt tggcgtcggc
     4681 cctgttgttg aatactcggt acaccggagc ggtcttggga ggacatacac cggtcgtccc
     4741 atccggcatc tggatcttga agacattgtc tgatt
//
LOCUS       DATIMH010000375         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2975315, whole genome shotgun sequence.
ACCESSION   DATIMH010000375 DATIMH010000000
VERSION     DATIMH010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2975315"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VLI90_06530"
     CDS             complement(<1..426)
                     /locus_tag="VLI90_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13897.1"
                     /translation="MILLSVVSPATSQVAQFEPAPAPLVLSASLPGDSLLRRSAPFML
                     AGGDTPSRDELFGDEQPASRDDLFGRAEATSRGPRISGFVDELAGYSYNGPGHWSRGV
                     TRLQLVAQGEFAAGIKWKASGRVDADPVYFLSNVYPDPVK"
     gene            complement(456..1229)
                     /locus_tag="VLI90_06535"
     CDS             complement(456..1229)
                     /locus_tag="VLI90_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV13898.1"
                     /translation="MTEPLIRIEKVYKHYRLGRQSVPALVDVTLDIEEGVFLAIAGPS
                     GSGKSTLLNIIGCIDTATHGRVAIDGKEVSGQSPDDLADLRARTIGFVFQTFNLLPVL
                     TAAENIEYPLLQFAELARKERRKRVAAMLELVGLAKHAAHRPNELSGGQRQRVAVARA
                     LVTRPRIILADEPTANLDSKTGDGILDLMKEINRTYRTTFVFSTHDRKVIAKADRLVR
                     MEDGHIRRLGVRTTANWVLVRARQERISTARRDRGRDPK"
     gene            complement(1394..2527)
                     /locus_tag="VLI90_06540"
     CDS             complement(1394..2527)
                     /locus_tag="VLI90_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV13899.1"
                     /translation="MALAGTTAVSGTAFAEFDLSNCNGTGLACPYVTYGDGNSYSLNL
                     NAYIYDAANGGGTGPGNPFYVNSSPGAIQGLVVNATGSNGQTVTTNFAGMDNAYPTPN
                     ETQSTVFFTMNKSQYPTLPIPGVSAPDDPGGVGQFTGDLADSWDTTVAALDAFLGAGN
                     TPIFFFNNNQVNSGATTNEDLAVWAQITLTGPNGAFAVFDFTNDGNVYGQPLVSPLDP
                     RFNGDVTAYTSTGAGPNAGTNASTDYVFAGGRLCIVPGTPYPALVDTVNGVCPAGTTT
                     IQNNLGANQAAYAVVFPELNAALATGLYTSMHVDLRFGCDPNTVGFNQDSTGNCLGRS
                     ANNGYEQLFIAAASQVINVPEPATLALLGLGLVIVGFARRKVH"
BASE COUNT          526 a          824 c          848 g          568 t
ORIGIN      
        1 cttgaccgga tccggataca cgttggaaag aaaatagacg gggtcggcat cgacgcgtcc
       61 gctcgccttc catttgatgc ccgccgcgaa ttccccctgc gcaaccagct gcagccgcgt
      121 gacgccccgt gaccaatgcc ccgggccgtt atagctgtag cccgctagct cgtcgacgaa
      181 gccacttatt cgcggtccac gggatgtcgc ctcggctctg ccgaagagat catcgcggga
      241 ggcgggttgc tcgtcgccga acagctcgtc gcgcgatggc gtgtcgccgc cggcgagcat
      301 gaaaggcgcg gaacgccgta acagggaatc gccgggaagc gatgcagaca gcacgagtgg
      361 tgccggcgcc ggttcgaatt gtgccacctg gctcgttgct ggcgaaacga cggacagtaa
      421 gatcatggcc gccggaaacc tcggtgcgag aagcctcatt ttggatctct cccgcgatct
      481 ctgcgcgctg ttgatattcg ctcctgtctg gcccgaacga gcacccaatt tgccgtggtc
      541 ctgaccccca gccggcgaat gtgtccgtct tccatgcgaa ccaaacgatc ggctttggcg
      601 atcacctttc ggtcgtgggt agaaaatacg aatgtcgtgc gatacgtgcg attgatttcc
      661 ttcatcaggt cgagtattcc atcgccggtt ttgctgtcga ggttcgcggt tggctcgtcg
      721 gcgaggatga tccgcggtcg cgttacgagt gcacgggcga cggcgacgcg ttgccgctgg
      781 ccccccgaaa gctcgtttgg tcgatgcgcc gcgtgctttg caaggccgac gagttcgagc
      841 atggctgcca cgcgcttgcg ccgctccttg cgcgcaagtt cggcgaactg cagcagcggg
      901 tattcgatgt tctccgcagc cgtaagcacc gggagcaggt tgaacgtctg gaacacgaat
      961 ccgatcgtgc gcgcccgcaa atcggccagg tcgtccggcg attgcccgga gacctctttg
     1021 ccgtctattg ccacgcggcc gtgcgttgca gtatcgatgc agccgatgat gttcagcaac
     1081 gtcgacttgc cgctgcccga gggccccgcg atggcgagga acacgccctc ctcgatatcc
     1141 agcgtaacgt cgaccagggc cgggacggac tgacgaccga gacggtaatg cttgtagact
     1201 ttctcgatcc gaatcaacgg ttcagtcatc ggagcgtacg tgtcctcgtg ccggcgcggt
     1261 gacgcctcag tgtctgaaca gcttatacgc tttggcgcgg gtcgcaaaac cccgaaatcg
     1321 aggccgatcg gtgctaccta aaaaaggcgg cgcccgaggc gccgcctttg acctaccgaa
     1381 tcgagcgaat gtgctagtgc acttttctcc gcgcgaagcc gacgatcacc agacccaatc
     1441 ccagaagtgc cagggtggca ggttcgggaa cgttgatgac ttgagaggca gcagcaatga
     1501 aaagctgctc gtacccgttg ttggcgctcc ggcccaggca gtttcccgtg ctgtcctggt
     1561 tgaagccgac ggtgttcgga tcgcagccaa agcgcaggtc tacgtgcatt gacgtgtaca
     1621 aaccggtcgc gagcgcggcg ttcaactctg gaaacaccac ggcgtatgcg gcctgattgg
     1681 cgcctagatt gttctggatc gtcgtcgtgc cggccgggca aacgccgttc acagtgtcca
     1741 ccaacgccgg atacggcgtc ccgggcacga tgcaaagcct gccgcccgcg aacacgtaat
     1801 cggtacttgc gttggtgcct gcattcgggc ctgctccggt agaggtgtaa gccgtgacgt
     1861 caccgttgaa acgcgggtcg agcggtgaga cgagaggctg cccgtagacg ttgccgtcgt
     1921 tggtaaagtc aaacaccgcg aacgccccgt tcgggccagt gagcgtgatc tgggcccaga
     1981 ccgcaagatc ctcgttcgtg gtggcgccgc tgttcacctg attgttgttg aagaagaaga
     2041 tcggtgtatt gccggcaccc aggaatgcgt cgagcgccgc aaccgtggtg tcccagctgt
     2101 cggcgaggtc gcctgtgaat tggccaactc caccgggatc gtcgggcgcg ctcacgcccg
     2161 gaatcggcaa cgtcggatac tgcgacttgt tcatcgtgaa gaaaacggta ctttgcgtct
     2221 cgttcggcgt tggataggcg ttgtccatgc ccgcaaagtt cgtcgtcacg gtctggccat
     2281 tcgaacccgt cgcgttcact accaagccct gaatcgcgcc cggtgacgaa ttgacgtaga
     2341 acggatttcc ggggccagta ccgccgccgt tggcggcgtc gtaaatgtac gcgttgagat
     2401 tgagcgagta tgagttgccg tcgccgtacg tgacatacgg gcacgccagt ccagtcccgt
     2461 tacagttact caagtcgaat tcggcgaacg cggtgccact gacggctgtc gtgcccgcca
     2521 aggccaagct tgtgatgatc gtcctaagct tcatacccct ccctgtggat gtgaatctgt
     2581 attgccaacc cggtgcacta caagcaagga cttggccaaa tctacaaagc tttatttttc
     2641 aatacgttga gacaatagta gacagcaccg ttgtaagaat ctccgacatc cacttcatgc
     2701 tcgtcattgt tcgcttgtgg gctacgacgc gttgagcgca gagatgtggc gcccgcggtg
     2761 agtcaa
//
LOCUS       DATIMH010000376         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_89117, whole genome shotgun sequence.
ACCESSION   DATIMH010000376 DATIMH010000000
VERSION     DATIMH010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89117"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1992)
                     /locus_tag="VLI90_06545"
     CDS             complement(<1..1992)
                     /locus_tag="VLI90_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSV13900.1"
                     /translation="MTRRFLPWCALLISIVCASCANQSAPTTSVAGISAPPRVVKNLS
                     PNWKFIRQDVPGAEQPAFDDSSWDSINLPHTWNARDGEDGTADVKANPRGDYYRGVGW
                     YRRQLTLDPNQVKGKSVFLRFDAASIEADVFVNGKHAGNHKGMFAAFCFDVTPLLNAS
                     GDNIIAVKVNNAMNPDIPPLNADFTFFGGLYRGVNVLVLDPLSITPLDDASSGVYVKQ
                     AKVDADHADLEITTKLRNADSATKSASVKCEVLDAGGNVVQSSNGNAQVAAGADADSV
                     QTIGIDHPHLWNAKKDPYLYQVRVTVSDGSNVVDRVTQPLGIRFFKADPDQGFFLNGA
                     SYPLHGVNRHQDRPDKGWAISPADHAEDFALIMEMGCTGIRLAHYQHAQEFYDLCDKG
                     GLVVWAEACLVNEVTASQAFDDVARQQVRELVKQNFNHPAICFWSLFNELRDPGGVKG
                     EQLEIQRKHQIDLVSSLNDEAHQLDPSRLTTGATNQPKPEYELNKITDVIAFNRYDGW
                     YRKAATDWPKELDDTKAALPGRAIGISEYGAGGGIHMHEANPPRKPKGTVQAWHPEEW
                     QCYVHEQAYGAMKQRPWLWGTFLWNMFDFAADNRHEGEHAGINDKGMVTYDRKVKKDV
                     FFFYKANWTQTPVTYIAERRHTDRVYRTAPVKVYSNATST"
BASE COUNT          384 a          711 c          624 g          460 t
ORIGIN      
        1 ggtcgaggtc gcgttgctgt agaccttcac cggcgccgtc cggtagacgc ggtcggtgtg
       61 ccggcgctcc gcgatgtagg ttaccggcgt ctgcgtccag ttggccttgt agaagaagaa
      121 cacgtccttc ttcaccttgc ggtcgtacgt gaccattcct ttgtcgttga tgccggcgtg
      181 ctcgccttcg tggcgattgt cggcggcgaa gtcaaacatg ttccacaaaa acgtgcccca
      241 cagccacggg cgctgcttca tcgcaccata tgcctgctcg tgcacgtagc attgccactc
      301 ttcgggatgc cacgcctgca cggtcccttt tggtttccgc ggcgggttgg cctcgtgcat
      361 gtggatgccg ccgccagcgc cgtattcgct gatgccgatc gcccggcccg gcaacgccgc
      421 cttggtgtcg tccaattcct tcggccaatc cgtcgccgcc ttgcgatacc aaccgtcgta
      481 gcggttgaac gcgatcacat cggtgatctt gttcagctca tattcgggct tcggttgatt
      541 cgtcgcaccg gtcgtcagcc gactgggatc gagttgatgt gcctcatcat tcagcgacga
      601 caccagatcg atctggtgct tgcgctggat ctccagctgc tcgcctttca caccacccgg
      661 atcgcgcagt tcgttgaaca agctccaaaa gcagatggcg ggatggttga agttctgctt
      721 gaccagctcg cgcacctgct ggcgggcgac atcgtcgaac gcctgcgatg ccgtcacttc
      781 gttgaccagg catgcctctg cccagacgac aagcccgccc ttgtcgcaca ggtcgtaaaa
      841 ctcctgcgcg tgctgatagt gggccagccg aatgccggtg cagcccatct ccatgatcag
      901 cgcgaaatcc tccgcgtgat ccgcgggcga aatcgcccaa cccttgtcgg gccgatcctg
      961 gtgccgattc acgccatgca gcggatacga agcgccattc aggaaaaaac cctgatccgg
     1021 atctgctttg aagaaccgaa tcccgagcgg ctgagtgacg cgatcgacga cgttcgaccc
     1081 atcgctcacc gtcacccgca cttgatacag atatggatcc ttcttcgcgt tccacaggtg
     1141 cggatgatcg atgccgatcg tctgcaccga atcggcgtcc gcccccgcgg ccacttgcgc
     1201 attaccgttg ctgctctgca cgacgtttcc gccggcgtcg agcacctcgc acttcacgct
     1261 cgccgacttc gtcgcgctgt cagcgttgcg cagcttcgtc gtgatctcca gatccgcgtg
     1321 gtccgcgtcc accttcgcct gcttcacgta cacaccgctc gacgcgtcat ccaacggcgt
     1381 gatcgacagc ggatcgagca ccagcacgtt caccccgcgg tacaacccgc cgaagaacgt
     1441 gaagtccgcg ttcagcggcg ggatgtccgg gttcatcgcg ttgttcacct tcaccgcgat
     1501 gatgttgtcg cccgaggcat tcagcagcgg cgtcacgtca aagcagaacg ccgcaaacat
     1561 gcccttgtga ttgccggcgt gcttgccgtt gacgaacacg tccgcctcga tcgacgccgc
     1621 atcgaagcgc aggaagacgg acttgccttt gacttggttc gggtcgaggg tcaactgccg
     1681 gcgataccag ccgaccccgc ggtaatagtc gccgcgggga ttggccttca cgtcggcggt
     1741 gccgtcttcc ccgtcgcggg cgttccacgt gtgcggcagg ttgatcgagt cccacgatga
     1801 atcgtcaaat gccggctgct cggcgcccgg cacgtcctgg cggatgaact tccaattcgg
     1861 actcagattc ttcaccaccc gcggcggcgc ggatatgccg gcaaccgacg tggtcggggc
     1921 cgactgattg gcgcacgaag cgcagacgat cgagatgagc agcgcgcacc acggcaaaaa
     1981 gcgtcgggtc atatttcctt ctccaagatt ttcagcaacc accggcggcg atgaaacgag
     2041 attgcgccgg cgatgcgcat cctactctac cgatgccgag acaagaggtt aacgtcggcg
     2101 cgattatttt caccagcggc gtgaggcaaa ttcgcttgtc gtttctggta tgagctacca
     2161 tgttcttccg tgaacctcc
//
LOCUS       DATIMH010000377         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5000245, whole genome shotgun sequence.
ACCESSION   DATIMH010000377 DATIMH010000000
VERSION     DATIMH010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5000245"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1227)
                     /locus_tag="VLI90_06550"
     CDS             complement(277..1227)
                     /locus_tag="VLI90_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV13901.1"
                     /translation="MTELANFSVPHASLDEGDSAPPPPPPAIVDVATSESQATTAPPE
                     PHVRIRPENAWSMLDLRELWQFRDLMRSLAMRDVKLRYRQTALGVIWVLFQPLLGAGI
                     FAVVFGLITKTPTFGTPHFLFAYGGMLMWNAFSGTLSKASTSLIGNTQLVSKVYFPRL
                     ILPLSTAMSTVIDFVIALALLFVLMPMFGIRSSTAILTLPLWFGFMVLLGMGLGLFCA
                     AMTVSYRDVQYIIPVLLPFLLYASPVGYSINLVPHKLKLWFHLNPMTALLEASRWALF
                     TTEGSPSWRAVIYSILVTIGIFMLGASVFVRMERKFADVI"
     gene            complement(1382..2524)
                     /locus_tag="VLI90_06555"
     CDS             complement(1382..2524)
                     /locus_tag="VLI90_06555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV13902.1"
                     /translation="MSAPPCLLVSGDFVKTGGMDRANHALASHLARSGREVHIVAFGI
                     DDELRRTPGIVFHPVPKPGGSYTLALPLLDGFGRRWAKRLTSRSPGARVIVNGGCSRW
                     GDVNWVHYVHAAHRPQVRRGMARWLASEWNHHRWLHDERAALTQARIVIANSQRTRRD
                     VIERLGVDPARVKVVYLGVDADRFAPATADEKMCERRALGLDEHRPIVAFIGAMSDRR
                     KGFDLLFDAWAMLCRDGNWDAQLLVVGAGAEVPAWQSAAAAVGVESRMRFLGLRDDVP
                     RILRASDLLVAPARYEAYGLSVHEALCCGVPAMVSESAGVAERFTPAMSGWLLPDRLT
                     PAHLAARLRDWQRGAIGGVDQLPALSNTLRAHTWDEMAAEISRFMS"
     gene            complement(2521..>3621)
                     /locus_tag="VLI90_06560"
     CDS             complement(2521..>3621)
                     /locus_tag="VLI90_06560"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSV13903.1"
                     /translation="ILAPVFWAPDMVRDPARLWRLLGILLVCNGVNASVGILQVYDPD
                     RWLPKEYSSIVMDTYGEALTFTGADGRQMVRPPGLSDSPGAVCGPAAAAALLGLIFCV
                     QPGLALWRRGGAAACAFAGIAAIYLSNVRSSLIVVLGSALAFVVVLYIQKERRRASML
                     LALAAGLVIGGLAFATALGGQGIFERFATLLEDDPRTVYYKNRGNQVHYAFDYYLAEY
                     PLGAGLGRWGMMRHYFGNEDNPRSPMIWAEIQFPAFILDGGIVLLVVYTLAVARSAWN
                     EFQIARKSTNPQLRGAAAAVFATNLGTIALMFSYVPFVAPIGVQFWFLAGCLHGATRA
                     DKSLQPKAPAGMLARRSTPMLSRRRRSIGAAV"
BASE COUNT          706 a         1197 c         1129 g          589 t
ORIGIN      
        1 cgatggcctc gcccagcgtg gtgtgcttgg gcgcgttatg gcggatcgtg taagccttgc
       61 ctacgttgcg ggcggagatg gcgatgtcgc tctgactcat gttaactgct ggttcggtta
      121 ccacggaggc acggagaaga gtttttaacc gcagatttcg cagattcaga aagagctttt
      181 tcaaatctgc gtcatccgcg aaatctgcgg ttcaaacgcc ttcgtctttt tcgtctgtgt
      241 ctccgtgcct cccgaggtta atcatcccca caactcttag atcacgtcgg cgaacttccg
      301 ttccatccgt acaaacacgc tggcgccgag catgaagatg ccgattgtga ccaggatcga
      361 atagatcacc gctcgccacg atggcgagcc ttcggtggtg aacagcgccc aacggctggc
      421 ttccagcagc gcggtcattg ggttcaggtg gaaccacagc ttcagcttgt gcggcaccag
      481 gttgatcgaa tatcccaccg ggctggcgta cagcaggaac gggagtaaca ctggaatgat
      541 gtactgcacg tcgcgatagc tgacggtcat cgcggcgcag aacagcccta ggcccatgcc
      601 cagcagcacc atgaagccga accagagcgg cagcgtgagg atcgcggtcg agctgcgaat
      661 gccgaacatc ggcatcaaca cgaacagcag tgccagcgcg atgacgaaat cgatcacggt
      721 ggacatcgcc gtggacagcg gcaggatcaa tcgcgggaag tagaccttgg aaaccagttg
      781 cgtgttgccg atcagcgagg tgctggcttt gctcagcgtg ccactgaagg cgttccacat
      841 cagcatgccg ccgtaggcga acagaaagtg cggcgtgccg aacgtcggcg tcttggtgat
      901 caacccgaaa acgacggcga agatgccggc gcccagcaac ggttggaaca gcacccagat
      961 gacgccgagc gctgtctgac ggtagcggag cttgacgtcg cgcatcgcca gcgaccgcat
     1021 gagatcgcgg aactgccaaa gctcgcgcag gtcgagcatc gaccaggcat tttccggccg
     1081 gatgcgcacg tgcggctcgg gcggcgcggt tgtcgcctgg gattcactcg tcgcaacatc
     1141 gacgatagcc ggcggcggtg gtggtggcgc ggaatccccc tcgtccagcg acgcatgtgg
     1201 aacagaaaaa ttcgccaatt cggtcatcac cttacaagcc tggttcgcac ccctacgtac
     1261 cattcgctca aaacaaccgg cgggttcgtc ttatgtagca cgggctccga gcctacggct
     1321 ctctcgcccg tgccgagccg tcaagcacgg gcgagacgcc catgctactt tcgatcccgc
     1381 atcaactcat aaatcggctg atttcggctg ccatttcatc ccatgtgtgg gcgcgcaacg
     1441 tattggataa cgccggcagt tgatcgacgc cgccgatcgc gccgcgttgc caatcgcgta
     1501 atcgcgctgc cagatgcgcc ggcgtcagac ggtccggcaa taaccagcca gacatggctg
     1561 gcgtgaaccg ttcggcgacg ccggcggatt cgctgaccat cgcggggacg ccgcagcaca
     1621 gcgcttcgtg cacgctcagg ccgtacgcct cataacgcgc gggggcgacc agcaggtcgc
     1681 tcgcccgcag gatgcgcggc acatcgtcgc gcaggcccag gaaccgcatg cgcgattcca
     1741 cacccaccgc cgccgccgcg ctttgccacg cgggcacttc tgcgccggcg ccgaccacca
     1801 gcagctgcgc gtcccaattg ccgtcgcggc acaacatcgc ccacgcgtcg aacagcagat
     1861 cgaacccctt gcggcgatcg ctcatcgccc cgatgaacgc gacgatcggc cgatgctcgt
     1921 ccaaacccag cgctcggcgc tcgcacattt tttcgtccgc cgtcgccggc gcgaatcgat
     1981 cagcgtcaac gccgaggtac acgaccttca cgcgcgccgg atcaacgccg aggcgttcga
     2041 tcacgtcgcg ccgcgtgcgc tgcgagttgg cgatgacgat gcgcgcctgc gtcagcgctg
     2101 cccgttcgtc gtgcagccag cgatgatggt tccactcgct ggccagccat cgcgccatcc
     2161 cacgccgcac ctgcgggcga tgggcggcgt gcacgtaatg cacccagttc acatcgcccc
     2221 agcgcgagca tccaccgttg acgatcaccc gcgcacccgg cgatcgcgag gtcaatcgct
     2281 tcgcccatcg ccggccaaac ccatcgagca gcggcaacgc cagcgtgtaa gacccgcccg
     2341 gcttgggcac cgggtgaaag acgatgcccg gcgttcgccg cagttcgtca tcaatgccaa
     2401 acgcgacgat gtgcacctcg cgcccgctgc gcgccagatg tgacgccagc gcgtgattcg
     2461 cccgatccat cccgccggtc ttcacaaagt cgccggaaac gagcaggcag ggaggagcgc
     2521 tcataccgcc gccccgatgg atcggcggcg ccgcgaaagc atcggtgtcg atcgacgcgc
     2581 cagcattcca gcgggcgctt tcggttgcag cgatttgtcg gcgcgcgtcg ccccgtgcag
     2641 acagccggcg aggaaccaga actgcacgcc gatcggcgcg acgaacggca cgtagctgaa
     2701 catcagcgcg atcgtgccca ggttcgtcgc aaacaccgcg gcggcggcgc cgcgcagctg
     2761 cgggttagtc gactttcgcg cgatctgaaa ttcgttccac gctgaacgcg ccaccgccag
     2821 cgtgtacacg accagcagca cgatgccgcc gtcgaggatg aacgcgggaa attggatctc
     2881 ggcccagatc atcggcgagc gcgggttgtc ttcattgcca aagtaatgcc gcatcatgcc
     2941 ccatcgcccc aggcccgcgc ctagcggata ttctgcaagg taatagtcga acgcatagtg
     3001 cacttggttg ccgcggttct tgtagtagac ggtgcgcggg tcgtcttcta gcaacgtggc
     3061 gaaacgttcg aagatgcctt gcccgccgag cgcggtggcg aaggcaagtc cgccgatcac
     3121 gagcccggca gcgagtgcga gcagcatcga ggcgcgccgg cgctctttct ggatgtacag
     3181 gacgacgacg aacgccagcg cggagccgag gacgacgatg aggctggagc gaacattgct
     3241 gagatagatg gcggcgatgc cggcgaaggc gcacgccgcc gcgccaccgc ggcgccacag
     3301 ggcaaggccc ggttgaacgc agaagatgag ccccagcaac gccgccgccg ccgctgggcc
     3361 gcacacagcg ccgggcgaat cgctcagccc cggcggtcgc accatctgcc ggccatcggc
     3421 gccggtgaac gtcagcgctt cgccgtaggt gtccatcacg atcgacgaat actccttcgg
     3481 caaccagcgg tccgggtcat acacctgcag gatgccgacg gacgcgttga cgccattgca
     3541 gaccagcagg atgccgagca gccgccagag gcgggcgggg tcgcgcacca tgtccggcgc
     3601 ccagaagacc ggcgcgagga t
//
LOCUS       DATIMH010000378         1820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4712630, whole genome shotgun sequence.
ACCESSION   DATIMH010000378 DATIMH010000000
VERSION     DATIMH010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4712630"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..>1820
                     /locus_tag="VLI90_06565"
     CDS             188..>1820
                     /locus_tag="VLI90_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSV13904.1"
                     /translation="MKHRHEIIVTLLAGCVFAGSAFATASTTAPATAPTTAPAPAPAG
                     SFSIGVDRITPPMITVSSAPVTVHVSAIGLATQPADWLNETYRWNFGDVTGATQPAID
                     PRTQQPVDLNTAIAAPVAAYLYESPGVYAITLTRITAAGEIRTYACSITVPVPKRNVY
                     YIASTGDDSASGVDPTRALRTAAAAVRRVADHTELRFQRGGVFAIDHAIDLTHRDVVL
                     DCYGDPSLPLPVIYRVAAASPATTQTTQATQPATMTTTSPTAGPFVNTWPGKTADVTI
                     RHLRFDSPYAIAHAGAYLYHTPAAYFGFLRGKNVTIADCEFANVFEGPHGDQTLSGAL
                     FLRNRQVQVLGIPGRTLWLEGSDIVAIGNIATNSINESPIRAADTGVVRGVIAFNDIA
                     QQLDAIEGRGGAKAAMTLRSLRDVQVCANHIANGELSFDPHTGDAFDQNVLTADNVIH
                     NSVLRVRPNCRSAIFRNNAIDIAGGPCICVTNGDGVAMWNADVRIEDNRGQGWQSNGR
                     MFLIDPCPPAAMRNCSMDAGKNAYTRIAPPPPPVAP"
BASE COUNT          307 a          705 c          515 g          293 t
ORIGIN      
        1 atgatgacgg ctcaggctgc atccaccgac gcatcttctt cctcccctgc cactcccgct
       61 gctgctgctg ccgccgccgc tgcatcctcc gcagcttcct catcctcctc tgcctcctct
      121 gcctctgcgg cctctgcgtc tctgcgttca ttgcctcttc tcttaccgcc aaagaaccca
      181 cgcacaaatg aaacaccgcc acgaaatcat cgtcaccctg ctggccggct gtgtgttcgc
      241 cggttcggct ttcgccaccg cttcgaccac cgcgccggcc acggcgccga cgactgcgcc
      301 agcgccggcg cccgccggtt cgttctcgat cggcgtcgat cgcatcaccc cgccaatgat
      361 caccgtctcg tccgcgccgg tcaccgtgca tgtgtcagcc atcggcctcg ccacccaacc
      421 ggccgattgg ctcaacgaaa cctaccgctg gaacttcggc gacgtcaccg gcgcgacgca
      481 gccggcgatc gacccgcgta cccagcagcc ggtggacctg aacaccgcca tcgccgcccc
      541 ggtggcggcg tatctctatg aatcgcccgg cgtgtacgcc atcacgctga cgcgcatcac
      601 cgccgccggc gaaatccgca cctacgcctg ctccatcacc gttcccgtgc cgaagcgcaa
      661 cgtgtattac atcgccagca ctggcgatga ttctgccagc ggggtcgatc caacccgcgc
      721 tctacgcacc gccgccgcgg cggtgcggcg cgtcgccgac cacaccgagc tccgcttcca
      781 acgcggcggc gtcttcgcga tcgatcacgc gatcgacctg acgcaccgcg atgtcgtcct
      841 cgattgctac ggcgatccca gcctgccgct gccggtgatc tatcgcgtcg ccgcggcttc
      901 gccggcaacg acgcagacca cgcaggcgac gcaaccggcg acgatgacaa cgacgtcgcc
      961 cacggcgggg ccgttcgtca acacctggcc cggcaagacc gccgacgtca ccatccgcca
     1021 cctgcggttt gactcgccgt acgccatcgc ccacgccggg gcgtaccttt atcacacgcc
     1081 agccgcgtac ttcggcttcc tgcgcggcaa gaacgtcacg atcgccgatt gcgagttcgc
     1141 caacgtcttc gaaggcccgc acggcgatca gacgctcagc ggcgcgctct ttctccgcaa
     1201 ccgccaggtc caggtgctcg gcatccccgg ccgcacgctc tggctcgagg gctcggacat
     1261 cgtcgccatc ggcaacatcg ccaccaacag catcaatgag tcgcccatcc gcgcggccga
     1321 caccggcgtc gtccgcgggg taattgcatt caatgacatc gcacaacagc tggacgccat
     1381 cgaaggacgc ggcggggcga aggccgcgat gaccctgcgg tcgctgcgcg acgtgcaggt
     1441 ctgtgccaac cacatcgcca acggcgaatt gtcgttcgat ccccacaccg gcgacgcgtt
     1501 cgaccagaac gtgctgacgg cggacaacgt gattcacaat tccgtcctgc gcgtccgccc
     1561 caactgccgc agcgcgatct tccggaacaa cgcgatcgac atcgccggcg ggccgtgcat
     1621 ctgcgtcacc aacggcgacg gtgtcgcgat gtggaacgcc gacgtccgca tcgaagacaa
     1681 ccgtggccaa ggctggcaaa gcaacggccg gatgttcctg atcgacccgt gtccgccggc
     1741 ggcgatgcgg aactgctcga tggacgcggg gaagaacgcg tatacgagga ttgcgccacc
     1801 gccgccgccg gtcgcgccgg
//
LOCUS       DATIMH010000379         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3554612, whole genome shotgun sequence.
ACCESSION   DATIMH010000379 DATIMH010000000
VERSION     DATIMH010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3554612"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VLI90_06570"
     CDS             <1..1676
                     /locus_tag="VLI90_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSV13905.1"
                     /translation="NRSAYGPGDEKRFAAGLDAAKVPVIIATEIDPSGKLGRFTPPTT
                     APTNFGVTTAPGGGFVKEYQPASYGHPSLALAAVTTAGFTPAPWTNHRFWLHYYGPHR
                     DAAGAFTFRYISAANVVMAGLLPKGDWGVKPEDFAGKIVLIGGIAAGTYDAKISPYGM
                     CPGIEVNATAIENMIHDQRVQPVAQQSVIALAFATALIAACASMFPRRMWIKLLAGFA
                     CMIAVLLLARFLFLRPEIHWLDPAAPLLAAAISIILALGWSYFVEDRQSRFFLRALSQ
                     CVSPKVAEELRQDPSRLMISTEKRELTILFSDIAGFTDISEALKEKVGRLLNYYLDEM
                     SGPVFASDGFLDKYIGDAVMAFWNAPLGQPDHAARACRAALAMKARLAEIQPQLAELG
                     APGLSSRIGINTGEATFGMMGSSYKFNYSTIGDPVNYASRLEGANKNYGSGIMLGEDT
                     AKAIANGGFVIRKLDLLKVQGKRPAEVYELLAEGPPDALLKLRVEGYEKAFERYLARD
                     WDAAERFLLDVLSQFKDDAPARMLLERVWAFRHDPPPLDWDGVYVAKSK"
     gene            1687..>2201
                     /locus_tag="VLI90_06575"
     CDS             1687..>2201
                     /locus_tag="VLI90_06575"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HSV13906.1"
                     /translation="MIKAIRRHPWVMRAAVTSIVIGAIGVGVVLAEDLWVNNVYVELH
                     QGPMGAFPVVPGVRVKQGEKLTVMERQDDWIHVKYNNVDGWVQESDLADKPVGASSMA
                     AFADSSSSGAGNAQAAKGFDEHEFAVAKNYKEEPFKNWMAMYDVSSPTTLVKPGDVKK
                     FMAAGHVGPVKS"
BASE COUNT          422 a          655 c          723 g          401 t
ORIGIN      
        1 acaaccgcag tgcgtacggc ccgggcgacg agaagcgttt cgccgctggg ctggacgcgg
       61 cgaaggtgcc ggtcatcatc gcgacggaaa ttgatccgtc ggggaagctg ggccgattca
      121 cgccgcccac caccgcgccg acgaacttcg gcgtcaccac cgcgcccggc ggtggattcg
      181 tcaaagaata tcaaccggca tcctacggtc acccgagcct cgcacttgcc gccgtcacca
      241 ccgcggggtt tacgccagcg ccgtggacga accaccggtt ctggctgcac tactacggtc
      301 cgcaccgtga cgccgccggc gcgttcacat tccgatatat ctccgccgcc aacgtcgtga
      361 tggcggggct actgcccaag ggggactggg gcgtgaagcc ggaggatttc gcgggcaaga
      421 tcgtgctgat cggcggcatc gccgcgggga cttatgatgc gaagatttcg ccgtacggca
      481 tgtgccccgg cattgaagtg aatgcgacgg cgatcgaaaa tatgatccac gaccagcgcg
      541 tgcagccagt cgcgcagcaa tcggtgatcg cgctggcctt cgcaacggcg ttgatcgcgg
      601 cgtgcgcgtc gatgttcccc cggcggatgt ggatcaagtt gctggcgggc ttcgcctgca
      661 tgatcgcggt gctgctgctg gcccgattcc tgttcctgcg accggaaatc cactggctcg
      721 accccgccgc gccgttactt gccgccgcca tctcgatcat cctggcgctg ggctggagct
      781 acttcgtcga ggatcggcag tcgcggtttt tccttcgcgc gctgagccag tgcgtgtcgc
      841 cgaaggtcgc ggaggaactt cggcaagatc ccagccgatt gatgatcagc accgaaaagc
      901 gcgagctgac gatcctgttc agcgacatcg cggggttcac cgacatcagc gaagcgttaa
      961 aggagaaggt cggccggctg ttgaactatt acctcgatga aatgtccggc ccggttttcg
     1021 ccagcgatgg ttttctggac aaatatatcg gcgacgcggt gatggcattc tggaacgccc
     1081 cgctgggcca gcccgatcac gccgcccgcg cctgtcgggc ggcgctggcg atgaaagctc
     1141 ggctggcgga aattcagccg cagctggcgg agctgggcgc gccgggcctg tcatcgcgca
     1201 tcggcatcaa caccggcgag gccaccttcg gcatgatggg ctcgagctac aaattcaatt
     1261 attcgaccat cggcgatccg gtcaattacg cctcgcgact cgaaggggcg aacaagaatt
     1321 acggctcggg catcatgctc ggcgaagaca ccgccaaggc gatcgccaac ggcggcttcg
     1381 tcatccgaaa gctcgatctg ctgaaggtgc aaggcaagcg gccggcggaa gtatacgagc
     1441 tgttggccga gggaccgccc gacgcgctgt tgaagctgcg cgtggaaggt tacgaaaaag
     1501 cgttcgagcg atacctggcg cgcgactggg acgcggccga gcggtttttg cttgatgttc
     1561 tcagtcagtt taaggacgat gcaccggccc gcatgctgct cgagcgggtg tgggcgtttc
     1621 gccacgaccc accgcccctg gactgggacg gcgtgtatgt ggccaagtca aaatgagggc
     1681 tcattcatga tcaaagcgat tcggcgtcac ccctgggtga tgcgtgccgc ggtgacgagc
     1741 atcgtcatcg gtgctattgg tgtaggtgtg gtgctggccg aggatttgtg ggtcaacaat
     1801 gtgtacgtcg agttgcacca gggaccgatg ggcgcgttcc ccgtcgtccc cggcgtccgc
     1861 gtgaagcagg gcgaaaaact cacggtgatg gaacgccagg acgactggat tcacgtcaaa
     1921 tataacaacg tagacggatg ggtgcaggag tcggacctgg cggacaaacc cgtaggcgca
     1981 tcgtccatgg cggcgttcgc cgacagctcc tcctccggcg ccggcaacgc gcaagccgcc
     2041 aagggattcg acgagcatga gttcgcggtc gccaagaatt acaaggagga accattcaag
     2101 aattggatgg cgatgtacga cgtcagttcg ccgacgacgc tggtgaagcc gggcgatgtc
     2161 aagaaattca tggccgccgg tcatgtcgga ccggtgaagt c
//
LOCUS       DATIMH010000380         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_90519, whole genome shotgun sequence.
ACCESSION   DATIMH010000380 DATIMH010000000
VERSION     DATIMH010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90519"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..901
                     /locus_tag="VLI90_06580"
     CDS             257..901
                     /locus_tag="VLI90_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13907.1"
                     /translation="MSLLWDFSTIADHLGQTLQQAEADLRLEQAVYGLDTRDEKALQS
                     LLCEGLRRWYEVAREVHYPSTFGKKLTHRQRCDLVLAPKDRPLRLDRNPPTLFDPPNQ
                     CEPKDALWLEVKIAYQYREGGVRHGGYGAQWRQAVVDDLRKMEAEPLIREAGLLLIVF
                     NESGQILQKDLELFEDVLAQKEVLAGFRHVRSVEILDRIGHRLCTAAIWPTLQR"
     gene            1159..1383
                     /locus_tag="VLI90_06585"
     CDS             1159..1383
                     /locus_tag="VLI90_06585"
                     /inference="COORDINATES: protein motif:HMM:NF010229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HSV13908.1"
                     /translation="MLTTLATSALLAQAAAFPTGLVSLAIVFFIIAIVAYVLGARGVA
                     GMSAGVGRTLLFVFLVLAVIIFIFSFVRTA"
     gene            1644..2069
                     /locus_tag="VLI90_06590"
     CDS             1644..2069
                     /locus_tag="VLI90_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSV13909.1"
                     /translation="MPKRERSAGVIVVKPAPSPEDHHYLLLDYGKHWDFPKGHVERGE
                     ELTQTALRELEEETGVKNVQLIAGFSHEIEYFFRNRRKELVHKSVWFCLAKTEKADVR
                     LSHEHVGYAFLPFDAALKRVTYPSAKSILREAEAFLAKQ"
     gene            2226..>3243
                     /locus_tag="VLI90_06595"
     CDS             2226..>3243
                     /locus_tag="VLI90_06595"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSV13910.1"
                     /translation="MKRRTPAAAALVGSALVSAIAAQQAGALTNPIAKGSLTLKLTTV
                     ATVNTAADSTPQDLTFEPGDASGRLFVATRNGTIRILSGGTLSSTPFLSLGSAGVSVY
                     TGGEGGLLGLAFSPNFTTDHKFYTFETEPFSTSGPAADYSVPEMFPTTSTNPNNQTTI
                     REWTVSANPAIANTTSRVLLRLNHPESNHQGGGLRFGPDGNLYMTLGDGGGGNDFNGS
                     VTSTTDGHNNATGNGQDTTVPFGKILRINPDPTARAGFITSTNGQYSIPSGNPFSSGA
                     NGNLKEIYAYGLRNPFRINFDSSNGSLYAADVGQSNREEVDIVSNNGNYGWPYFEGTR
                     DNQGS"
BASE COUNT          613 a         1042 c          959 g          629 t
ORIGIN      
        1 ctggcgatcg catatccgca cgcggccgcc acgttttgga cgttggcgat ctgctgcgcc
       61 gcgctgcggt acgtgctcga tgcacattgg ccgagcgacg tcctgggcgg cgtcgccttg
      121 ggctacgccg ccgccgtggt aacatggcgg gcgtttggaa tgtgattgag atgtagcacg
      181 ggcgtctcgc ccgtgtcgaa cggcgcacgg cacgggcgag acgcccgtgc tacgcgaaga
      241 tgtttctcgc tgtccgatgt cactcctctg ggacttctcc accatcgctg atcacctcgg
      301 tcagacgctt caacaagccg aggcggacct gcggctggaa caagcggtct atggcttgga
      361 cacgcgggac gagaaggccc ttcaatcttt attgtgcgag ggcctgcgac ggtggtacga
      421 ggtcgcgcgc gaggtgcatt atccttccac cttcggaaaa aagctgacgc atcgccagcg
      481 gtgcgatctg gtgctggcgc cgaaggatcg gccgttgcgg ctcgatcgga atccaccgac
      541 gttgttcgat ccgccgaatc agtgcgagcc gaaggacgcg ctgtggctgg aggtgaagat
      601 cgcgtatcag tatcgcgaag gcggcgttcg gcacggcggg tatggcgcgc agtggcgaca
      661 ggcggtggtg gatgatctgc gcaagatgga ggccgagccg ctgatccgcg aggcaggatt
      721 gctgctgatc gtgttcaacg agtcggggca gatcctgcag aaggacctgg agttgttcga
      781 agacgtattg gcgcagaagg aagtgctggc ggggttccgg cacgtgcgca gcgtcgagat
      841 cctcgatcgc atcgggcacc gcctgtgtac ggcggcaatc tggcccaccc tgcagcgatg
      901 accggtgtcg cgggtgtcgc cggagcggtc tcttccactt cgttccgttc aaaacgcggg
      961 tattttttgg acgttttccg ccgctccagc gcgtttcaag cgaactttgc aatgattgtg
     1021 cgcatacggc acgcgcgttg caaaactgta tctccgtagc cggcacgtcc ggcgagaaat
     1081 ttcgaaaatg ttttgccccg gccccaagcg cgttgcttgc gcggtccggt ggaactggtt
     1141 agttcaaagg agaacgccat gcttaccaca ctcgcaacca gcgcactgct ggcccaggcc
     1201 gcggcctttc cgaccggcct cgtttcgctg gccatcgtct tcttcatcat cgccatcgtc
     1261 gcctacgtgc tgggcgcgcg tggcgtcgcc ggcatgagcg ctggtgttgg ccgaaccttg
     1321 ctgttcgtct ttctggtcct ggccgtgatc atcttcatct tcagcttcgt ccgcacggcg
     1381 taacgactgc gttgcgcgtg accgaggaat tgaagattgc acgatcgtat gtaccggccg
     1441 tgacgcgttc tccttgcacg cgttgcggcc ggtttgtttt tttcttcttc actttatcct
     1501 gagcgaagcg gcgcagccga gcagtcgaag gatccagctt cgaacgccgg tgaacctttg
     1561 ccgaagcgat tggttcaccg acataggaag caatatcctt cgactgcgcg gctttgccgc
     1621 ttcgctcagg ataaacttaa gacatgccca agcgagagcg atccgccggt gtcatcgtcg
     1681 tcaagcctgc tccatcgccc gaagatcacc actacctcct gctggactac ggcaagcact
     1741 gggactttcc aaagggccac gtcgagcggg gtgaagaatt gacgcagacc gcgctgcgcg
     1801 agctggaaga agagacaggc gtcaagaacg tgcagctgat cgccggcttc tcgcacgaga
     1861 tcgaatattt tttccgcaat cgccgcaagg aactggttca taagtccgtc tggttctgcc
     1921 tcgccaagac agaaaaggcg gatgtgcgcc tcagccacga acacgtcggc tacgcgtttc
     1981 tacccttcga cgcggcactc aagcgcgtca cctaccccag cgccaagagc atccttcgcg
     2041 aagccgaagc gttcctggcg aagcagtagc catcttcgcg tctgccttcg cgtcttcgcg
     2101 tcttcgcgtc ttcgcgtctt cgcgtcttcg cgatcgcatt tacctttttt ccttgcccct
     2161 ccccaaccgc caacgctatt gtgacgcctg ttcccacacc aactcgcaca cacaggacgc
     2221 gcatcatgaa gcgacgcacc cccgcggcgg cggcccttgt cggatcggct ctggtttcgg
     2281 cgatcgccgc tcaacaagcc ggcgctttaa ccaacccgat cgccaaggga tcgttgacgc
     2341 tgaagctgac gacggtcgcg acggtgaaca ccgccgccga cagcacgcct caggatctga
     2401 ccttcgagcc cggcgacgcc agcgggcgat tgttcgtcgc cacgcgcaac ggcacgatcc
     2461 gcatcctcag cggcggcacg ctgtcatcga cgccgttcct cagcctcggc agcgccggtg
     2521 tcagcgtcta caccggcggc gagggcgggt tgcttggcct ggcattcagc ccgaacttta
     2581 cgaccgatca caaattctac acgttcgaga cagaaccgtt cagcaccagc ggccccgccg
     2641 ccgactacag cgtgcccgag atgtttccca ccaccagcac gaatcccaat aaccagacga
     2701 cgatccgcga gtggacggtc agcgccaatc cggccatcgc caacaccacc tcgcgcgtgc
     2761 tgctgcgatt gaatcatccc gagagcaacc accagggcgg cgggctgcgc ttcggtcccg
     2821 acggcaacct gtatatgacc ctcggtgacg gcggcggcgg caacgatttc aacggcagcg
     2881 tcaccagcac gaccgatggc cacaacaacg cgaccggcaa cgggcaggac acgaccgttc
     2941 cgttcggcaa gatcctccgc atcaatcccg atccgaccgc gcgcgcgggt tttatcacca
     3001 gcaccaacgg tcaatattcg atcccgagcg gcaatccgtt cagcagcggc gccaacggca
     3061 acctgaaaga gatctacgcc tacggcctgc gaaatccctt ccgcatcaac ttcgattcca
     3121 gcaacggatc gctctacgcc gccgacgtcg gccagtccaa ccgcgaagag gtggacatcg
     3181 tcagcaacaa cggcaattac ggctggccat acttcgaagg cacgcgcgat aaccagggca
     3241 gcg
//
LOCUS       DATIMH010000381         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2109660, whole genome shotgun sequence.
ACCESSION   DATIMH010000381 DATIMH010000000
VERSION     DATIMH010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109660"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VLI90_06600"
     CDS             complement(<1..311)
                     /locus_tag="VLI90_06600"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HSV13911.1"
                     /translation="MPMPTSQQTSDLIIGVDVGGTFTDLVALDPTTGQLNVIKIPSTP
                     PDFHLAVLDAVAQAQKSPPSPGTPGEGRGEGDSKFQMALDKPSHPHPNPLPAYRERGQ
                     I"
     gene            326..2212
                     /locus_tag="VLI90_06605"
     CDS             326..2212
                     /locus_tag="VLI90_06605"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSV13912.1"
                     /translation="MTLRSRLAVSIVTLLLLCAGCNSQPAATAKHADQAPATQASSEF
                     PTTSNPRDTLPFLASDALAGRAPGTPGIERAGDFLATEFARIGLQRLPGTDGYFQPFP
                     MALSTSLAPGTGLSINDKPLQLSTDFSPLSLTGEGPFSGHVVFAGYSIVNPKFDEYAG
                     IDAGGKVVLAMRQEPVDQNKKSRFAAPNQNWSDGAFFSAKAKAAAAHGAVALLLVSPP
                     SSGGDDQVLPFFGDTAGSGAKIPVIQISRRVADLLLEMGGAPDLKSLQAEIDSTLSPR
                     SVELKDVEVAGDVAVKRSTASVRNVLAYLPGAGEHADEFVVVGAHYDHLGTGQLGHML
                     GPVGSIYHGADDNASGTAAVLELAERMKAAGPLPRSVLFALFTGEEEGLIGSDYFIKH
                     PPVPLDKIVAMINLDMVGRMKNDTVLMGGWGTAPIWESMMKQAAADVGVKTQSFEKGG
                     LGPSDHMSFALKKIPVLFLFTGLHKDYHRPTDTADKINYAGIDRAVDLMQRLVAATAA
                     MPRQQYDGSNDSKATMAFATGHGAAGVGGGQAALGVVPDYSSMDATDGVHITGVGGGS
                     PAEAAGLQPGDVLTRWNDKPLNNLQDLSDDLAQANPGDAVQITVLRDGKTVTVRAKLG
                     TRKK"
BASE COUNT          406 a          642 c          814 g          401 t
ORIGIN      
        1 atgatctgtc ccctctcccg gtacgccggg agagggttag ggtgagggtg actgggtttg
       61 tcgagagcca tttgaaattt tgaatcaccc tcacccctgc cctctcccgg cgtaccggga
      121 gaggggggag atttttgcgc ctgcgcgacg gcatctaaga ccgccagatg aaagtcaggc
      181 ggcgtcgagg ggattttgat gacgttgagt tgtcccgtcg ttggatcgag cgcgaccagg
      241 tctgtgaacg tcccaccgac atcgacgccg atgattaagt cgctcgtttg ttgggaggtg
      301 ggcatgggca tagaatcggc cgccgatgac gctccgatca cgcctggccg tttcgattgt
      361 caccttactg ctgctgtgcg ccggctgcaa ttcgcaaccc gcggcgacgg cgaagcacgc
      421 ggaccaagcg ccggcgacgc aggcatcgtc tgagtttccg acgacgagca acccgcgcga
      481 cacgctgccg ttcctggcgt ccgatgcgct ggccggccgc gcgccgggca cgccggggat
      541 cgagcgcgcg ggcgattttc tggcgacgga attcgcgcgg atcgggttgc agcgattgcc
      601 gggaacagat ggatatttcc agccgtttcc gatggcgttg tctacgtcgc tcgcgccggg
      661 cacgggcctg tcgatcaacg acaagccgct gcagctgagc accgatttct cgccgctgtc
      721 gttgaccggc gaggggccgt tcagcgggca cgtggtgttc gcggggtatt cgatcgtgaa
      781 tccaaagttc gatgaatacg ccggcatcga cgccggcggc aaggtcgtgc tggcgatgcg
      841 gcaggagcca gtggatcaga acaagaagag ccgattcgcg gcgccgaatc agaactggtc
      901 cgacggcgcg ttcttcagcg ccaaggcgaa ggcggcggcg gcgcacgggg cggtggcgct
      961 gctattggta tcgccgccga gcagcggtgg cgatgatcag gtgctgccgt tcttcggcga
     1021 caccgcgggc agcggggcga agatcccggt gattcaaatc agccggcgcg tcgccgatct
     1081 tctgctggag atgggcggcg cgccggattt gaaatcgctg caggcggaaa ttgattcgac
     1141 gctgtcgccg cggtcggttg agttgaagga cgtggaagtc gccggcgacg tggccgtcaa
     1201 acgatcgact gccagcgtgc ggaacgtgct ggcgtatctg ccgggcgccg gcgagcacgc
     1261 ggatgaattc gtcgtcgtcg gcgcgcatta cgatcacctc ggtaccgggc agctggggca
     1321 catgctcggt cccgtcggca gcatctatca cggcgcggat gacaatgcat ccggcacggc
     1381 ggcggtgctg gagctggccg agcgcatgaa agccgcgggg ccgctgccgc gatcggtttt
     1441 gttcgcgctg ttcaccggcg aagaagaggg actgatcggg tccgattact tcatcaagca
     1501 cccgccggtg ccgttggaca agatcgtcgc gatgatcaat ctcgacatgg tcgggcggat
     1561 gaagaacgac acggtgctga tgggcggctg gggcaccgcg ccgatctggg aatcgatgat
     1621 gaagcaggcg gccgccgacg tcggtgtgaa gacgcagagc ttcgagaaag gcgggctggg
     1681 gccgagcgat cacatgtcgt tcgcgctgaa gaaaatcccc gtgctgtttt tgtttaccgg
     1741 cctgcacaag gactaccatc gcccgacgga cacggccgac aagatcaact acgccggcat
     1801 cgatcgcgcc gtcgatctta tgcagcggct ggtcgcagcg acggcggcga tgccccgtca
     1861 gcagtacgat ggtagtaatg acagtaaagc gacgatggcg ttcgcgacgg gccacggcgc
     1921 cgcgggtgtc ggtggcgggc aggcggcgct gggcgtcgtg ccggattaca gctcgatgga
     1981 cgcgaccgat ggcgtgcaca tcaccggcgt cggcggcggc agccccgccg aagccgcggg
     2041 gcttcagccg ggcgacgtgc tgacgcggtg gaatgataag ccgctgaaca atctgcagga
     2101 tctatcggat gatctggcgc aggcgaatcc tggggatgcg gtgcagatca cggtgctgcg
     2161 cgatgggaag acggtgacgg tgcgggcgaa gttggggacg cggaagaagt aaaatgtcat
     2221 cctgagcgca gcacgtactc gtgcggagtc gaaggatctg gcc
//
LOCUS       DATIMH010000382         1708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5576992, whole genome shotgun sequence.
ACCESSION   DATIMH010000382 DATIMH010000000
VERSION     DATIMH010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5576992"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VLI90_06610"
     CDS             <1..1274
                     /locus_tag="VLI90_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HSV13913.1"
                     /translation="AQERMVVSVPKQHVDELLKLAAGEDVEATVFGTFGTADRQLILN
                     YRGEEVGRLSMRFLHDGIPMPTRKAVVTESRAPQKSPRMATRGLSSAGAVHDFRDRLL
                     KTLAHPNIASKHWIIRQYDHEVQGGSVIKPLIGPQQIGPSDAAVVRPKLSSTRGVAIG
                     CGLSPQIVDSYEMSIAAIDEAIRNVVAVGADYDRIAILDNFCWPSVDDERTMGTLVRA
                     CEACYDAAKAYAIPFISGKDSLHNQFTNQETGEVLRIPSTLLISAIGIVEDIRKCVTM
                     DLKNRTARVCLVSAKDPADLKSLAATHRLVAQSIAAGEIVACHDISDGGVAVAAAEMC
                     IASGLGMIVGEELVADEEAFAERAGRYLIELREPTLADELRQRFSSAAEVVEFGLVQH
                     LRKLTVTSTKSRVEEIGLEDLTNAWRGTLDW"
     gene            complement(1429..>1708)
                     /locus_tag="VLI90_06615"
     CDS             complement(1429..>1708)
                     /locus_tag="VLI90_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13914.1"
                     /translation="MKPFYRYSQSGEINFEVEDKDAKIREIAEVYKKAKTDYLDGITV
                     DLGEWWFNVRKSNTEPLLRLNLEAKSPEQLEEKMRELKRLLGEPAHGH"
BASE COUNT          321 a          510 c          551 g          326 t
ORIGIN      
        1 aagcgcagga gcggatggtg gtgtcggtgc caaagcagca tgtggacgag ctgctcaaat
       61 tggccgcggg ggaggatgtg gaagcgacgg tgttcggcac gttcggcacg gcggatcgcc
      121 agttgatcct gaattatcgc ggagaggaag tcgggcgatt gtcgatgcgc ttcctgcacg
      181 atgggatccc gatgccgacg cggaaggcag tcgtgacgga gtcgcgcgca ccgcagaaaa
      241 gcccacggat ggccacccgt gggctttcgt cagccggcgc cgttcacgat ttccgtgacc
      301 gattactcaa gacgctcgcg catcccaaca tcgccagcaa gcactggatc atccgccagt
      361 atgaccacga ggtgcagggc gggtccgtca tcaagccgct gatcggcccg cagcaaatcg
      421 gcccgtccga cgcggccgtg gttcgcccca agctgtcgag cacgcgcggc gtcgcgatcg
      481 gctgcggact gtcgccgcag attgttgact cgtacgagat gtcgattgcc gcgatcgacg
      541 aggccattcg caacgtcgtc gccgtcggcg ccgactacga tcgcatcgcg atcctcgata
      601 atttctgctg gccgagcgtg gacgacgagc gcacgatggg cacgctggtc cgcgcctgcg
      661 aagcctgcta cgacgcggcc aaggcgtacg ccatcccgtt catcagcggc aaggattcgc
      721 tgcacaacca gttcaccaac caggaaaccg gcgaggtgct gcgcatcccc agcacgctgc
      781 tgatcagcgc gatcggcatt gtcgaggaca tacgcaagtg cgtgacgatg gacctgaaaa
      841 accgcaccgc ccgcgtgtgc ctcgtttcgg caaaagatcc ggcggatctg aagtcactcg
      901 cagcgacaca tcgattggtc gcgcagtcga tcgccgcggg cgagatcgtc gcgtgtcacg
      961 acatcagcga cggcggcgtc gcggttgccg cggcggaaat gtgcatcgcg tcgggactgg
     1021 gaatgatcgt cggcgaagaa ctggtcgcgg acgaggaagc gttcgccgaa cgtgcggggc
     1081 gatatctgat cgaactgcgc gagccgacgc tggcggatga attgcgacaa cggttttcat
     1141 ctgccgccga ggttgtggag tttggattgg tgcagcacct gcggaagttg acggtgacgt
     1201 cgacgaagtc gcgggtggag gagattgggc tggaagatct gaccaacgcg tggcgtggta
     1261 cgttggattg gtagtttctg ccttgtcctg agcgaagcac gtacgcgtgc gtagtcgaag
     1321 aatctagccg aggagtaggt gaagcgttcg gatcgaaagg ttcaccgact gccggccaga
     1381 tccttcgact ccggcggagt accgcctgcg ctcaggataa aggcagcgtc aatgcccatg
     1441 cgccggttcc cccagcagtc gcttcagctc ccgcatcttc tcctccagct gctccggtga
     1501 cttggcttcc aggttcagcc gcagcagcgg ctcggtgttg ctcttgcgga cgttgaacca
     1561 ccattcgccc aaatcgaccg tgatcccatc gaggtaatcc gtcttcgcct tcttgtacac
     1621 ctctgcgatc tcgcggattt tcgcgtcctt gtcctccacc tcgaagttga tctcgccgct
     1681 ctggctatag cggtagaacg gcttcatc
//
LOCUS       DATIMH010000383         5956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_91011, whole genome shotgun sequence.
ACCESSION   DATIMH010000383 DATIMH010000000
VERSION     DATIMH010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91011"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /gene="aroE"
                     /locus_tag="VLI90_06620"
     CDS             <1..1217
                     /gene="aroE"
                     /locus_tag="VLI90_06620"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HSV13915.1"
                     /translation="LILSSHDFTGRPARLTNIVNDLFESSADVAKIVWTARTIRDNLE
                     AFEILQHKQKPTIALCMGEAGLISRVLAKKFGAFLTFASLDASGGTASGQIPVADMKR
                     LYRWDAIDADTRVYGVVANPVAHSMSPAIHNAAFDRVGYNGVYLPLLVEGSYESFKAF
                     LESFLHFPGLHLSGLSITLPHKENALRYLQEKNAEVEELAARIGAVNTIVIERDADDQ
                     PKLRGFNTDYAAILDCITDKLQITREQLADYRVAVIGAGGTGRTAVAALAHYGATVVI
                     YNRTKERAEALAAEFNGHRGKVVSARIEKLCDSCCQIYINTTSVGMHPNVDQSPLGGT
                     PPKFADDTLVFDAIYNPMKTKLLKDAEAAGAKTIGGVEMFVRQAAGQFTAWTGKPAPT
                     DVMREIVESRLR"
     gene            1389..2096
                     /locus_tag="VLI90_06625"
     CDS             1389..2096
                     /locus_tag="VLI90_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13916.1"
                     /translation="MLRLRIGVVAGWLALAALVLLGRAWWSSYQTAERLTLARHARLV
                     VISDSGCVAIGLVWSPIATGQLPATRPVYRFRHEQFDSNLFRQMWRAPFGRFAHGWWL
                     GDSYWMQHAEGIILPYWLVTMLVFFIPAGLVVKRCGDRAKRTAAQPPPRVCLRCGYDL
                     SVTPTDSPCPECGLAAHRSVIDHVDPADCRPGWVRWIAVGSLLLLASYLVFGMYFVFA
                     FTIPRDSKCGASTRWPG"
     gene            2066..2647
                     /locus_tag="VLI90_06630"
     CDS             2066..2647
                     /locus_tag="VLI90_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13917.1"
                     /translation="MWGFNAMAWLMLLATVLNLAGCIMLARDDRPRAFAWRRFMLRWS
                     LQVLPAFGMAGALVEVLEYTLMPMPSPAMQSVSAVMSLPLLLCPTITFIRLRQLALRL
                     NRRRLAEHIGIVAAGAGASLLLMVVAVAFVSDHIRRADAVFYAFVVLPVALVALFNLW
                     ALLMLFVITPAFFNSARQAAARWRAADAARVGV"
     gene            2928..4085
                     /locus_tag="VLI90_06635"
     CDS             2928..4085
                     /locus_tag="VLI90_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSV13918.1"
                     /translation="MTLAAPVQSAEDPDIELLKAGYVLEESVLERMRTIGVRNIFVDY
                     PALDELDRHLKPLLSEPRRQMYGQIKQTFALAQEHLQPADLSYQDYYNSVRELMFTLM
                     SQGRHPIYLEQLSRLGGDTVAHAASVAHLSLLLGIKLERYLINQRRRLPIAHAKEVIN
                     LGVAGMLHDIGKLCLPAHLQNCTCLDEIKSDADRFEFQTHPRLGYDLVRMGLEPSAAS
                     AVLNHHQHYDGSGFATSVDKQGRPRVLSRTQIHVFARILFVADLYDRLAAVRGTTERR
                     SNLQVLWVMRTKFASWCDPVVLQALHAIAPPFPPGSRLTLSDCATVLVTIDDPADPFH
                     PIVKRLAADGWTLEEPSIDLGAPGMPTVVNLNGMAVAPFLPREDAMAFMRG"
     gene            complement(4251..4523)
                     /locus_tag="VLI90_06640"
     CDS             complement(4251..4523)
                     /locus_tag="VLI90_06640"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HSV13919.1"
                     /translation="MLKRTITISVCALLAMGLLAKAEDTATTQPAIAAPTTAPVPFNK
                     YCAVNTKDLVDPKVTLLYNGKVYGFCCEDCIKDFQKDPEKYAAKAK"
     gene            complement(4534..4794)
                     /locus_tag="VLI90_06645"
     CDS             complement(4534..4794)
                     /locus_tag="VLI90_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HSV13920.1"
                     /translation="MAHIIAEPCIGTKDTACVAVCPVDCIHPTKENPKFAEAEMLYID
                     PDVCIDCGLCVDECPVKAIFPQEDLPAEWSHFIEKNAEYFKK"
     gene            5095..>5956
                     /locus_tag="VLI90_06650"
     CDS             5095..>5956
                     /locus_tag="VLI90_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV13921.1"
                     /translation="MTPLRFAIAGCGGIAGLHAECLRRLASEGLAQLVGGFDRTERRR
                     AEFGAKWSVPMVASMEELLARDEVDAVIITTPSGTHGELAAQVANSGRHVLVEKPLDL
                     RLNKADAAIAAAKRAGVTLGGVSQQRFIPAVAAVKRAIDAGYFGQIVLVHSETPWYRP
                     QSYYDSAGWRGTWAFDGGVLANQGTHMIDRLLWLAGDVDQVLSATLECGRGRNVEVET
                     LAVATIRLFNGALGTITGTTLAYDGLPQRIIICGTEGSCAFSGDGLVAFKTLRPMTGD
                     APVLSSSEGQS"
BASE COUNT         1017 a         1783 c         1974 g         1182 t
ORIGIN      
        1 acctaattct cagcagtcac gacttcaccg gtcgcccggc tcggttgacg aacatcgtca
       61 atgatctctt tgagtcctct gccgacgtcg ccaaaatcgt ctggaccgcc cgcacaatcc
      121 gcgacaacct ggaagccttc gagatcctcc agcacaaaca gaagcccacg atcgcgctgt
      181 gcatgggcga agctggcctc atctcgcgcg tgctggcgaa gaaattcggt gcgtttttaa
      241 ctttcgcttc actcgatgcg tccggcggca cggcttccgg gcagatcccc gtcgcggaca
      301 tgaagcggct gtaccgctgg gacgcgatcg acgccgacac gcgcgtctac ggcgtcgtcg
      361 caaatccggt ggcgcattcg atgtcgccgg cgattcacaa cgcggcgttc gaccgcgttg
      421 ggtacaacgg tgtttacctg ccgctgcttg tcgaaggcag ctacgaaagc ttcaaagctt
      481 ttctcgaaag ttttctgcac ttccccggcc tgcacctgtc ggggctgtcg atcaccctgc
      541 cgcacaagga gaacgcgctg cgttatctgc aggaaaagaa cgcggaggtc gaagaactcg
      601 ccgcgcggat cggcgcggtg aacacgatcg tcatcgagcg cgacgccgac gatcagccga
      661 aacttcgcgg gttcaacacc gactacgcgg cgatcctcga ttgcatcacc gataagctgc
      721 agatcacgcg cgagcagctg gcggactatc gcgtcgccgt catcggcgcc ggcggcaccg
      781 gccgcacggc cgttgctgcg ctggcgcact atggcgcgac cgtcgtcatt tacaaccgca
      841 cgaaggagcg cgccgaagcg ctcgccgcgg agttcaacgg ccaccgcggt aaggtcgtct
      901 cagcgcggat agaaaagctg tgcgacagct gctgtcagat ctacatcaac acgaccagcg
      961 tgggcatgca tccgaacgtc gatcaaagcc cgctcggcgg cacgccgccg aagttcgccg
     1021 atgacacgct ggttttcgat gcgatttaca acccgatgaa gacgaagctc ctgaaggatg
     1081 ccgaggccgc gggggcgaag acgatcggcg gcgtagagat gtttgtccgc caggccgcgg
     1141 ggcagttcac cgcgtggacc ggcaagcccg cgccgacgga tgtgatgcgc gaaatcgtgg
     1201 agagccggct gcgctgatgc gcccaccacg ccgggttcgg agcgtactcg cgacgacccg
     1261 gatctcttct ctcgcgccgc agaatccggg tcgtcggagt ccctccgaac ccggggtgag
     1321 aagagttgaa ttcaccacca cttccatacg cggccgcgta aaatgtttct ccgatgcgat
     1381 tgctacgcat gttgcgactt cgaatcggcg tggtcgccgg ttggctggcg ttggccgccc
     1441 tggtgctgct cggccgggcg tggtggtcaa gctaccaaac ggccgagcgc ctcacgctgg
     1501 cgcgacatgc tcggctggtg gtgatctcgg attccggctg cgtagcgatc ggcctcgtat
     1561 ggtcgccgat cgcaaccggc cagttgcctg ccacgcgacc cgtataccgc tttcggcacg
     1621 agcaattcga ttctaattta ttccgtcaaa tgtggcgggc gccgtttggc cggttcgcac
     1681 acggctggtg gttgggcgat tcatattgga tgcaacacgc agagggaatc atccttccgt
     1741 attggctggt gacgatgctc gttttcttca tcccggcggg gctggtggtg aagcgatgtg
     1801 gtgatcgtgc caaacgaacg gcggcgcagc cgccgccgcg ggtgtgcttg cgctgcgggt
     1861 atgacctgtc cgtgacgccg acagattcgc cgtgtcccga gtgcgggctg gcggcgcatc
     1921 ggtcggtgat cgatcacgtc gatcccgccg actgccggcc cggatgggtg cggtggatcg
     1981 cggtcggctc gctgctgctg ctcgcgtcat acctggtgtt cggcatgtac ttcgtcttcg
     2041 cgttcacaat cccgcgggat tcgaaatgtg gggcttcaac gcgatggcct ggctgatgct
     2101 gctggcgacg gtgttgaacc tggccggctg catcatgctc gcgcgcgacg atcgcccgcg
     2161 cgcgtttgcc tggcggcgat tcatgcttcg ctggtcgctg caggtattgc cggcgttcgg
     2221 gatggcgggg gcgctggtgg aagttcttga gtacaccctg atgccgatgc cgagcccggc
     2281 gatgcaaagc gtgtcggcgg tgatgtcgct cccgctgctg ttgtgcccga cgatcacgtt
     2341 catccgcctg cggcagctgg cgctgcggct gaaccggcgg cggttggcgg aacacatcgg
     2401 catcgtcgct gccggggcgg gggcctcgct gctattgatg gtcgtggctg tcgcgttcgt
     2461 gtccgatcac attcggcggg cggacgcggt gttttacgcg ttcgtcgtcc tgccggtggc
     2521 gctggtggcg ctgttcaact tgtgggcgct gctgatgctg ttcgtgatca cgccggcgtt
     2581 tttcaattcg gcgcgacagg ccgcggctcg atggcgcgcc gccgacgcgg cgcgcgtggg
     2641 ggtgtgactt cggaataacg cccacggctc tgccgtggga cagtattctg gaaaacggtc
     2701 ccacgtcaga gccgtgggct tttgttttgt catccaccgc gcccgcgcac gaccgataac
     2761 gcgcggcgtg cgtgatccac atcgcacagc ttccgcatct tctcaagcaa tccgcttccc
     2821 gcgcagtttc gctggtcttc ctcgcttttc ttcgccgccg gtgtatcgca tctgaatcgt
     2881 gggagaccgc cgtgttgatc gttcccattc aagaagcccg tccgggcatg acgctggctg
     2941 caccggtgca gagcgcggag gatccggaca tcgagctgct caaggccggc tacgtgctgg
     3001 aagagagtgt gctcgagcgg atgcgcacga tcggcgtccg caacattttc gtggattatc
     3061 cggcgctgga tgagctggac cggcatctga agccgctgct cagcgagccg cggcgtcaga
     3121 tgtatgggca gatcaagcag acgttcgcgc tggcgcagga acacctgcag ccggccgacc
     3181 tttcgtacca ggattattac aacagcgtgc gcgagttgat gttcacgctg atgtcgcagg
     3241 ggcggcatcc gatttattta gagcagctgt cgcgcctcgg cggcgacacc gtcgcgcacg
     3301 cggcgtcggt ggcgcacctg tcgctgctgc tcggcatcaa gctggaacgt tatctcatca
     3361 accagcgccg gcggttgccg atcgcgcacg cgaaggaagt aatcaatctc ggcgtggcgg
     3421 gcatgctgca cgacatcggc aagttgtgcc tgccggcgca cctgcagaac tgcacgtgcc
     3481 tggatgagat caagtccgat gccgatcgtt tcgaattcca aacgcacccg cggctggggt
     3541 atgacctggt gcggatgggc ctcgagccgt cggcggcgtc ggcggtgctg aatcatcacc
     3601 agcattacga cggcagcggg ttcgccacgt cggtggacaa gcagggccgg ccgcgcgtgc
     3661 tgtcgcgcac gcagattcac gtcttcgcgc gcattctgtt cgtggccgat ctatacgatc
     3721 gcctggcggc ggtgcgcggc accaccgagc ggcggagcaa cctgcaggtg ctgtgggtga
     3781 tgcgaacgaa gttcgcgtcc tggtgcgacc cggtggtgct gcaggcgctg cacgcgatcg
     3841 ccccgccctt cccgccggga tcgcggttaa cgctcagcga ttgcgcgacg gtgctggtga
     3901 cgatcgacga tccggccgac ccgtttcatc cgatcgtgaa gcgattggcc gcggatgggt
     3961 ggacgttgga ggagccgtct attgatctgg gcgcgccggg gatgccgacg gtcgtaaacc
     4021 tgaacggcat ggcggtggcg ccgttcctgc cgcgcgagga cgcgatggcg tttatgcggg
     4081 ggtagtgcgt ggaacaccag acctgaatga cgaaattcga atccccgaat gaaatccgaa
     4141 tgacgaaatt cgaacgacga aacgtgcgcg atcgcatggt ttcgccattc gtgcttcatc
     4201 attcggattt acttcgatga ttccagtttc ggatttcgtc attccgaaag tcacttcgcc
     4261 ttcgccgcgt acttctccgg gtccttctgg aagtccttga tgcagtcttc gcagcagaag
     4321 ccgtaaactt tgccgttgta gagcagcgtc accttgggat cgaccaggtc cttggtgttg
     4381 acggcgcaat acttgttgaa cggcaccggc gcggtagtcg gggcggcgat cgccggctgc
     4441 gtggtcgcgg tgtcttcggc tttggcgagg agacccatcg ccagcagcgc gcagaccgag
     4501 attgttatcg tgcgtttcag catatcgttt cgcctacttc ttaaaatatt ccgcgttctt
     4561 ctcgatgaag tggctccact cggccggcag gtcttcttgc gggaagatcg ccttcaccgg
     4621 gcattcgtcc acgcacaggc cgcagtcgat gcacacgtcc gggtcgatgt agagcatctc
     4681 cgcttcggcg aacttggggt tttccttggt gggatgaatg caatcgaccg ggcagacggc
     4741 aacgcatgcc gtgtccttcg tgccgatgca gggttctgca ataatgtggg ccatgacgct
     4801 gaaacgctcc ttacaactgc gttgtgagtt tccagagcag ggattatagg gatcgatggc
     4861 ggctgtgaca aacgacgata aatcgtccgg cgtgggtgag atgggacgag gtggattggg
     4921 aagtagttag cgttgagccg ccgcagcctc gcggcgcgaa cgttaggtaa gtcacacccg
     4981 ctgccgcttg cggcttagca gatcgagtac gcttcgtttg ccaacggcgg cgattggggc
     5041 gtgcttgatc tgctaagccg caagcggcca aagaggacag gagcaagcga acagatgacg
     5101 ccgctgagat tcgccatcgc cggttgcggg ggaatcgctg ggttacacgc ggaatgtcta
     5161 cggcgattgg cgagcgaagg gctcgcgcag ctggtcggtg ggttcgaccg gaccgagcga
     5221 cggcgcgcgg aattcggtgc gaagtggagc gtgccgatgg tcgcgtcgat ggaggagctg
     5281 ctggcgcgcg atgaggtcga tgccgtcatc atcaccacgc ccagcggcac gcatggcgag
     5341 ttggcggcgc aggttgccaa cagtggccgg catgtgctgg ttgaaaagcc gctggacttg
     5401 cggctgaaca aagcggacgc ggcgatcgcc gcggcgaagc gcgccggcgt cacgctgggc
     5461 ggggtgtcgc agcagcggtt catcccggcg gtggccgcgg tgaagcgcgc gattgacgcg
     5521 ggatatttcg ggcagatcgt gctggtccac agtgaaacgc catggtatcg gccgcagtcg
     5581 tattacgact cggccgggtg gcgcggcacc tgggccttcg acggcggcgt gctggcgaac
     5641 cagggcacgc acatgatcga ccgcctgcta tggctggccg gcgatgtcga ccaagtgctc
     5701 agcgcgacgc tcgaatgcgg ccgcgggcgg aatgtcgaag tcgaaaccct cgccgtcgcc
     5761 accatccgat tattcaacgg cgcgctcggc acaatcaccg gcaccacgct ggcgtacgat
     5821 ggcttgccgc aacgcatcat catctgcggc accgaaggca gctgcgcctt cagcggcgat
     5881 ggactggtcg cgttcaaaac gctgcggccg atgacaggcg atgcgccggt cctctcatcc
     5941 agcgaaggcc aatccc
//
LOCUS       DATIMH010000384         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2398826, whole genome shotgun sequence.
ACCESSION   DATIMH010000384 DATIMH010000000
VERSION     DATIMH010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398826"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..848
                     /locus_tag="VLI90_06655"
     CDS             63..848
                     /locus_tag="VLI90_06655"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV13922.1"
                     /translation="MTDPIRFEDGAAYERYMGTWSRLAGETFLDWLAPRSGLRWLDIG
                     CGNGAFTEMIIERCAPAAVDGIDPSDAQLGYARTRPAGRVAKFHRADAMALPFADDSF
                     DAAVMPLVIFFVPDPAKGIGEMARVVSPGGAVAAYAWDILGGGFPYEALREQMRAMGL
                     TVPLPPSTEAAGIDALRELWTGAGLQAVETREIVVQRSFSNFDDYWATVLGGPSVGPQ
                     LAAMTTADVDAIKRKMRAALPTDPTGRITCSARANAVKGRAPK"
     gene            complement(994..1389)
                     /locus_tag="VLI90_06660"
     CDS             complement(994..1389)
                     /locus_tag="VLI90_06660"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSV13923.1"
                     /translation="MEIKRVGSRASAKGPADWFTGTVRIDPLLDPPDPARVGGASVTF
                     EPGSRTAWHTHPLGQSLIVTAGCGWAQREGGPREEIRPGDVVWFAPGEKHWHGATATT
                     AMTHIAIQEKLNGSPVQWMEKVTDEQYGG"
     gene            complement(1539..1976)
                     /gene="mutT"
                     /locus_tag="VLI90_06665"
     CDS             complement(1539..1976)
                     /gene="mutT"
                     /locus_tag="VLI90_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HSV13924.1"
                     /translation="MKRVDVALAIVLRGSRVLIARRKSTGPFASLWEFPGGKREPDET
                     IEACLHRELWEELAIRAKPITALAPIEHDYPQVHVCLHPYLCRHESGEPQAIACDEVQ
                     WVEPVELRAYPFPPANGPLIEGIIATLSEAGLPQRGSKESLNP"
     gene            2376..>2981
                     /locus_tag="VLI90_06670"
     CDS             2376..>2981
                     /locus_tag="VLI90_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13925.1"
                     /translation="MSTTRGQFKATVLANVPLCREHFRLVLRADGFPPTEPGQFVQVA
                     CRDLDRSMSDAVELDWQPGDRLSAEMDLELRAPLAVLRRPFSLAGRRDVGGGAVELDI
                     IHRVVGVGTHWMSQLQSGDAVHLIGPLGNTFTLPGDGEIALLIGGGVGIPPMIYLAEA
                     LARRGNAAVAFAGALTRDLLPLMITGEPAGAEPRPIIDEVAR"
BASE COUNT          476 a          945 c         1004 g          556 t
ORIGIN      
        1 cccggcgtgg gggggagcaa cgacgcttat cttcccgcga cgtccgacat aaagcctcaa
       61 ttatgactga cccaatccgc ttcgaagacg gggccgcgta cgaacgctac atgggcacgt
      121 ggagccggct cgcgggcgaa acgtttctcg actggctcgc gcccaggtcc ggcttacgct
      181 ggctcgatat cggctgcggc aatggcgcgt tcaccgagat gatcatcgag cgctgcgctc
      241 cggccgccgt ggatgggatc gatccgtctg acgcgcagct cgggtacgcg cgcacgcggc
      301 ccgcggggcg ggtggcgaag ttccatcgtg ccgatgcgat ggcgctgccg ttcgccgatg
      361 actcgttcga cgccgcggtg atgccgttgg tcatcttctt cgtgcccgac ccggcgaagg
      421 gcatcggcga gatggcccgc gtcgtcagcc ccggcggggc cgtggcggcg tacgcgtggg
      481 acatcctcgg cggcgggttc ccatacgagg ctttgcggga acagatgcgc gcgatgggcc
      541 tcaccgtccc gctgccgccg agcacggagg cagctggcat cgacgcgctg cgcgagttgt
      601 ggaccggcgc tggattgcag gcggtcgaaa cgcgcgagat cgtggtgcag cgcagcttct
      661 cgaatttcga cgactattgg gccacggtcc tcggcggccc cagcgtgggg ccgcaactcg
      721 ccgcgatgac gaccgcggat gtcgatgcga tcaagaggaa gatgcgggct gcgttaccga
      781 cggatcccac cggcaggatt acgtgcagcg cccgcgcgaa tgcggtgaag gggcgcgcgc
      841 cgaaatagca gggcgcttct tcgttgcgta gcggtagccc cgcaggtgca cgcgttttct
      901 gtcgaagtgc ggaagaacgt gcccctacgg ggctaccgct acgcggcgcg aagtcttcgg
      961 cgctcgcacg tttgcgtttg gaatcaaccg cgatcacccg ccgtactgct cgtcggtcac
     1021 cttctccatc cactgcaccg gactgccgtt cagcttttcc tggatcgcga tgtgcgtcat
     1081 cgcggtcgtc gcggtggcgc cgtgccagtg tttttcaccc ggggcgaacc agacgacgtc
     1141 gccggggcgg atctcttcgc gcgggccgcc ttcgcgttgc gcccagccgc agccggccgt
     1201 cacgatcagc gactgcccga gcgggtgcgt gtgccacgcg gtgcgggagc cgggttcgaa
     1261 cgtgacgctc gcgccgccga cgcgagcggg atccggcgga tccagcagcg gatcgatgcg
     1321 aacggtgccg gtgaaccagt ctgcggggcc cttggccgat gcgcgcgagc cgactcgttt
     1381 gatttccatg gggtgctcct tcgcccgcga ctgtacgcgg gtggggaagg atctgaatag
     1441 cgggattttg tcccgtaaca gcccaggccg gaaagccggg gttcggactg cgagtggcgc
     1501 catccgcaga tcgatgcccg accttgcggc ctgggcgatc aaggattgag cgactcttta
     1561 gatccgcgct gaggtaaacc cgcctccgac agtgtcgcga tgatcccctc gatcagcggt
     1621 ccgtttgccg gtgggaacgg gtacgcgcga agttcgaccg gctcaaccca ttgtacctca
     1681 tcgcacgcga tcgcctgcgg ctcgccgctc tcgtgccggc acagatacgg gtgcaggcac
     1741 acgtgcacct gtgggtagtc gtgttcaatc ggcgccaggg ctgtgatggg tttcgcacgg
     1801 atcgccagct cttcccacag ctcgcggtga aggcaggctt cgatggtttc atcgggctcg
     1861 cgcttgccgc cgggaaactc ccacaggctg gcgaacggtc cggtggattt tcggcgggcg
     1921 atgagcaccc gcgagccgcg caggacgatg gcgagggcga cgtctacacg cttcatatgg
     1981 ccggtccggc gaaacgatat cgggcccggt gtcaagcgca aaatcggggc cgcgctgctg
     2041 ccaaaaaacc ttcctcttcc ggtgcgcggc gggctgtcga gccgagaatg gcgatgcttg
     2101 taaagataac ataattagat acttatgcga tatatcgaat tcgcttccca gccaaaacgc
     2161 ttgattgaca gccttttgga gcaagggtat agtcacgccc gttgcagttg ggaaaccggc
     2221 tgcaatccca accatgagtt cagcggcttt tataagccgt ggcgcaactt ccggaggtgg
     2281 ataggagcca cccctccgga agttcttttt ttggcgtcgc tcatcataca cgtcgccgtt
     2341 tccttcctga tgaccttccg tacaatccgc cccgcatgag caccacgcgc gggcaattca
     2401 aggcgacggt gttggccaac gtgccgttgt gccgcgagca ctttcggctg gtcctgcgcg
     2461 ccgacggttt cccaccaacc gagccggggc agttcgtgca ggtggcctgt cgcgatctgg
     2521 atcggtcgat gagcgatgcc gtcgaactgg attggcaacc gggcgatcgg ttgtcggcgg
     2581 agatggacct tgaactccgg gcgccgctgg ccgtgctgcg ccggccattc tccttggccg
     2641 ggcggcgcga cgtgggcggt ggggcggttg aactggacat catccaccgc gtcgtcggcg
     2701 tcggcaccca ctggatgagc caacttcaat ccggcgacgc cgttcatctc attggcccac
     2761 tcggcaatac cttcacgttg cctggcgacg gtgagatcgc gctgctgatc ggcggcggtg
     2821 tcggcattcc gccgatgatc tacctggccg aagctttggc acgccgcggg aacgcggccg
     2881 tcgccttcgc cggcgcgctg acgcgcgacc tgctgccgct gatgatcacc ggtgaacccg
     2941 ccggcgctga accgcgtccg atcatcgacg aagtcgcccg g
//
LOCUS       DATIMH010000385         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2978106, whole genome shotgun sequence.
ACCESSION   DATIMH010000385 DATIMH010000000
VERSION     DATIMH010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2978106"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VLI90_06675"
     CDS             complement(<1..637)
                     /locus_tag="VLI90_06675"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HSV13926.1"
                     /translation="MRLNTILALTIVLLPLTTHAATTQPFILGADISWVPEDEADGAT
                     YFDHGVQKDIFQILHDNQFNMIRLRVFVHPHAPHGYAAGKAEAFCDLAHTLAMAKRAQ
                     AAGMGLLISLHYSDTWTNPEGHPKPHAWDGMTVEQLAGAVHDHTRDVLAALKDQGTPA
                     DMVAIGNEVTHGMLWPEGRVWSHIPSGNAQTDAANTNLGPGNGNYDNFATFL"
     gene            851..1918
                     /locus_tag="VLI90_06680"
     CDS             851..1918
                     /locus_tag="VLI90_06680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSV13927.1"
                     /translation="MSTTSAPTPAQTAAQTAASTVLAPAKEDEYNRTHLNYRQPMPRP
                     KVRGPVIDCHNHLFAARHAKGWYECCDHYGIDYCVTMAPLEEAVVLQRDWGYRTKFIA
                     VAAWGDQSPRWVDNWLIRIESFYNMGSRVAKFHAAPGTMAMRGVRLDDPRMRRIMDEV
                     VARGMILMSHVGDPDTWYNGKYAADRAKYGTRDEHYKMWEGLLAAYKHHPWWGAHLGG
                     NPEDLPRLQYLLDTYPNLHLDLSATRWMVREISARRDQAREFVIRNQDRLMWGSDQVS
                     GDDRGFDFLASRWWAHRKLWETAYIGPSPIFDPDLPGDQQPMLRGLALPDGVLQKIYH
                     DNVVNLLARVGVQFEPVPLAA"
     gene            complement(1949..3613)
                     /locus_tag="VLI90_06685"
     CDS             complement(1949..3613)
                     /locus_tag="VLI90_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13928.1"
                     /translation="MLDRPDARPAARPVAGFVLLIALAALCAGGKAILHDTMDPDCFW
                     HLRVADQLHREGIGPIVDHISFASLKTPWTPYSWLAELAMRAIWNFGDYRAAVVVQAL
                     MQSAIIAFMAMACVELQRQPQAGGEPRYFSAALATFTGGFLTLAFLSFRPVTTAFVLL
                     WACTWLVLRDRRMNENSRAIWLLVPITALATNIHLFAFFIPAVVLAMLLGAIYERRVS
                     TSMQASPTPDLQRTRDEPDASRAPQNPGRRSTSEPGVGVACELSRRIRRYLILLIAVS
                     LAYLATPMLPGTMRTVMFYGARDEMVRNGRIAEMQFFARGGFGIASAIIVAGIVACVI
                     WHHRRLRLGEIICFIAGLALLFKYARFSPVFAMAAAPALAATLPRLRDEVLGKRLITA
                     VMALVLVAGVVRVAMKLPRPNQTLCAWLGRHGPDAPSYPCSAADYVWNSVTPANGKII
                     NEFGWGGYLGWRLQGRFQVLMDGRTQCFSSEFWHAVYLDGEDARRKYLAKLKVDAAIL
                     PAQKSQFHDALQSFGWISVYKDKQSEVMVPAPPRRTIPYREPPLAA"
BASE COUNT          703 a         1130 c         1265 g          614 t
ORIGIN      
        1 tgagaaacgt cgcgaagttg tcgtagttcc cgttgccggg cccgaggttc gtgttggccg
       61 cgtcggtctg cgcgttgccc gatggaatgt gcgaccacac tcggccctcg ggccagagca
      121 tgccgtgcgt gacctcgttc ccgatcgcca ccatgtccgc cggcgtgccc tggtccttca
      181 gcgcggccag cacatcgcgc gtgtgatcgt gcaccgcgcc cgcgagctgc tcgaccgtca
      241 tcccgtccca cgcgtgcggc ttgggatggc cttcgggatt cgtccacgtg tccgaataat
      301 gaaggctgat gagcaacccc atccccgccg cttgggcgcg cttcgccatc gccagcgtgt
      361 gcgccaaatc acaaaacgcc tccgccttcc ccgcggcgta gccgtgcggc gcgtgcggat
      421 ggacgaacac ccgcaggcgg atcatgttga actgattgtc gtgcaggatt tggaagatgt
      481 ccttctgcac gccgtggtcg aagtaggtcg cgccgtcggc ttcgtcttcg ggaacccagg
      541 agatgtcggc gccgaggatg aacggctgcg tcgtcgctgc gtgggttgta agcggcagca
      601 ggacgatcgt gagggcgagg atggtgttaa ggcgcatgta agtcctcatc gaagaccatg
      661 gagaaacgac acggtggcgc gtcatcataa gtggacgacg ccgagcttca tcgccgcttg
      721 cggcttagcg tgtgttgtct ggcggaagaa cagacgctaa accgcaagcg gcggccgctg
      781 tcaagcggcg gccgctgtca agcggcggcc gctcgacatg ccgggagtgc gagcataggc
      841 tccagcaggc atgtcaacca caagcgcacc cactcccgca caaaccgccg cgcagaccgc
      901 ggcttccacc gtgctcgcgc cggcgaagga agatgaatac aaccgcacgc atttgaacta
      961 ccgccagccg atgccgcggc cgaaggtgcg cgggccagtc atcgattgtc acaaccatct
     1021 gttcgcggcg cgccacgcga agggatggta cgaatgctgc gatcattacg gaatcgacta
     1081 ttgcgtgacg atggcgccgc tggaagaggc ggtggtgctg cagcgcgact ggggttatcg
     1141 cacgaagttc atcgcggtgg cggcgtgggg cgaccagagc ccgcggtggg tggacaactg
     1201 gctgatccgc atcgaatcgt tctacaacat gggcagccgg gtggcgaagt ttcacgcggc
     1261 ccccggcacg atggcgatgc gcggcgttcg gctggacgac ccgcgcatgc ggcggatcat
     1321 ggacgaggtg gtcgcccgcg ggatgatcct catgtcgcac gtgggcgatc ccgacacgtg
     1381 gtacaacggc aagtacgccg ccgaccgagc caagtacggc acgcgcgacg agcattacaa
     1441 gatgtgggaa ggcctgctgg ccgcgtacaa gcatcatccg tggtggggcg cgcacctggg
     1501 cggcaatcca gaagacttgc cgcgtttaca atatttactc gatacgtatc cgaacctgca
     1561 cctggacctg agcgcgacgc gctggatggt gcgcgagatt tccgcccggc gtgaccaggc
     1621 ccgcgagttc gtcatccgca accaggaccg cttgatgtgg ggcagcgatc aagtgagcgg
     1681 cgacgaccgc gggttcgact tcctcgccag ccgatggtgg gcgcaccgca agctgtggga
     1741 aacggcgtac atcggcccga gcccgatttt cgatccggat ttaccgggcg atcagcagcc
     1801 gatgctgcgc ggcctggcgc tgccggatgg ggtgttgcag aagatttatc acgacaacgt
     1861 ggtgaacctg ctggcgcgcg tgggagtgca gtttgaaccg gtgccgcttg cggcttagcg
     1921 tctgtttttg ctgggagaga aaaagacgct aagccgcaag cggcggttcg cgatatggaa
     1981 tggttcgtcg cggcggcgcc gggaccatca cttctgattg cttgtctttg tacacgctga
     2041 tccatccgaa gctctgcagc gcatcgtgga actggctctt ctgtgccggg aggatcgcgg
     2101 cgtccacttt tagcttggca agatatttcc gccgggcgtc ttcgccgtcg aggtacaccg
     2161 cgtgccagaa ctcgctggag aagcattgcg tgcggccgtc catcagcacc tggaagcggc
     2221 cctgcaatcg ccagccgaga taaccgcccc agccgaactc gttgatgatc ttgccgttcg
     2281 cgggcgtgac ggagttccag acgtaatcgg cagcgctgca gggataagac ggggcatccg
     2341 gcccgtgccg gccgagccag gcgcagagcg tctggtttgg acgtggcaac ttcatcgcga
     2401 cgcgaacgac tccggcgact aagaccaacg ccatgacggc ggtgatcagc cgtttgccta
     2461 aaacttcatc gcgaagccgc ggcagcgtcg ccgcaagcgc gggggcggcg gccatcgcga
     2521 agacgggtga gaaccgcgcg tacttgaaca gcagcgcgag ccctgcgatg aagcagatga
     2581 tctcgcccag gcgcagccgg cggtgatgcc agatgacgca cgcgacgatg ccggcgacga
     2641 tgatagccga ggcgatgccg aacccgccgc gggcgaagaa ctgcatctcg gcgatccgcc
     2701 cgttgcggac catctcatcg cgggcgccgt agaacatcac cgtgcgcatc gtgcccggca
     2761 gcatcggcgt cgcgaggtat gcgagggaga cggcgatcag caggataagg tatcgccgga
     2821 tgcgccgaga tagttcgcag gcgactccca cgccgggttc ggaggtactc cgacgacccg
     2881 gattctgcgg cgcgcgagaa gcatccggct cgtcgcgagt acgctgcaaa tccggcgtgg
     2941 ggctcgcctg cattgaagtg ctgacgcgac gttcgtagat cgcgccgagc aacatcgcca
     3001 gcaccaccgc ggggatgaag aacgcgaaga ggtggatgtt cgttgccagc gcggtgatcg
     3061 gcacgagcag ccagatcgcg cgcgaatttt cgttcatacg gcgatcgcgc aggaccagcc
     3121 aagtgcacgc ccacagcagc acgaaggcgg tggtaacggg gcggaagctg agaaaggcga
     3181 gcgtgaggaa tccgccggtg aacgtggcga gggcagcgct gaagtagcgc ggctcgccgc
     3241 cggcctgcgg ttggcgctgc agctcgacgc acgccatcgc catgaaggcg atgatggccg
     3301 actgcatgag cgcctggacg acgaccgcgg cgcggtagtc gccgaagttc cagatggcac
     3361 gcatggccaa ctcggccagc cacgaatacg gcgtccacgg cgtcttcagc gatgcgaagg
     3421 aaatgtgatc gacgatcggc ccgatgcctt cgcggtgcag ctgatcggcc acccgcaggt
     3481 gccaaaagca gtcggggtcc atcgtgtcgt gcaggatcgc tttgccgccc gcgcacagcg
     3541 cggctaaggc aatcaacagc acgaaccccg cgaccgggcg cgcagcagga cgagcatcgg
     3601 gacgatcgag catcgtgttt acattgtcgg caaatgcgat gcagcgctcg cggaatggtc
     3661 tgcgtaatag cccaggccgg aaggccgggg atcggactgg aagcggcgcc gc
//
LOCUS       DATIMH010000386         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2400155, whole genome shotgun sequence.
ACCESSION   DATIMH010000386 DATIMH010000000
VERSION     DATIMH010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2400155"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1052
                     /locus_tag="VLI90_06690"
     CDS             270..1052
                     /locus_tag="VLI90_06690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSV13929.1"
                     /translation="MSDKNRDILSDAIGRNSAMVLSLPSAGMLRHHKSRFLNETDDGL
                     WIESVPAEHVLIDDLIAGGKQCAVSFKSGDQKVSFVAKVLRVDPEYRVNADMTVCAVL
                     LERPPQVKAVQRRNAYRARVREDSELSVQVWRIPEHVRVKDKPPRAVELALTVRDLSV
                     GGVGVIVLPQGDQPPKVLPGERVRVQIRFKDGEPCVVEGRMRTPRQAPAAAGDKPQPI
                     QTGVQFQGLESGLEGRQMLTELTKIVSALHLEEVRRHRMGIA"
     gene            complement(1176..1637)
                     /locus_tag="VLI90_06695"
     CDS             complement(1176..1637)
                     /locus_tag="VLI90_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HSV13930.1"
                     /translation="MRLTDILKPENIKVPLEAKTKNDAIAELVQVLAASGQVTDPKKV
                     LDAVLEREATRTTGIGNGLAIPHGKCPGTKDLVMAIGKPAQPIDFQSIDGRPVSVIWM
                     LASPPEKTGPHIHALARISRLMTIDKFQQALRLAKTPQEVFDAIVAQEAAL"
     gene            complement(2069..>2767)
                     /locus_tag="VLI90_06700"
     CDS             complement(2069..>2767)
                     /locus_tag="VLI90_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSV13931.1"
                     /translation="SAGFEGGMILLAAAIAIGKALDTLIRGLPLHMEALGVGVVLMVI
                     ALVVNAAVGWFLIRTGKRLSSITLEADGRHLITDAVTSSVALVALVLVRISGWTRIDP
                     IAAIGAAFYIAYTGIGLLRRSAAGLMDQQDVDDAAMLRRILDSHVGERGSEPRICSYH
                     KVRHRHSGRYHWVDFHMMVPAYWDIEQGHRAASAIEYEIEQALGEGNATAHVEPCAEA
                     GCSTCDSAKPASLQ"
BASE COUNT          487 a          867 c          885 g          528 t
ORIGIN      
        1 cgcgcccgcc ggtgatccac gcgaaattgt tccgccggcc cagcagcacg ccatctaacc
       61 cgtggcgctc gaggaactcc gccagccgct tttgcttgat gtccagttca ttgtcgggca
      121 tggcccaagg cttacccgaa aggcggggca atgagtaggt ctgataaccc gcggcgtcct
      181 cgatatgtct tatgtatgcc ggtccatcga ccgatgattg tgacggggac gcgccctcac
      241 atttttttgc tggtcgggat gctcttcaca tgtcagacaa gaatcgcgac atattaagcg
      301 atgcgatcgg ccggaactcg gcgatggtgt tgtcgctgcc gtcggcgggg atgctgcggc
      361 atcacaagag ccggttcttg aacgagaccg acgacggcct ctggatcgag tcggtgccgg
      421 ccgagcacgt gctgatcgat gatctgatcg ccggtggcaa acagtgcgct gtgtcgttca
      481 agtctggcga tcagaaggtc agcttcgtcg cgaaggtgct gcgcgtcgat ccggagtatc
      541 gggtgaacgc ggacatgacg gtgtgcgcgg tgctgctgga gcgcccgccg caggtgaagg
      601 cggtgcagcg gcgaaacgcg tatcgtgcgc gcgtgcgcga agacagcgag ctgtcggtgc
      661 aggtctggcg gattcccgag cacgttcgcg tgaaggacaa accgccgcgt gcggtcgagc
      721 tggcgttgac cgtgcgcgac ctcagtgtcg gcggcgtcgg agtcatcgtg ctgccgcagg
      781 gcgaccagcc gccgaaggta ctgccgggcg agcgcgttcg cgtgcagatt cgcttcaaag
      841 atggcgagcc gtgcgtggtc gaaggccgca tgcgcacccc gcgccaagcc cccgcggccg
      901 cgggcgacaa accgcaaccg attcagaccg gcgtgcagtt tcaagggttg gaatcaggtt
      961 tggaggggcg gcagatgctg acggagttaa cgaagatcgt cagcgcgctg catctcgaag
     1021 aagtccggcg gcatcggatg ggcattgctt aactacgcgc gtcatcggtc tgcgaagccg
     1081 caagcggcag gcttctctac aattgacctt cattaagctt cgagccagat ccttcgactt
     1141 cgcggctgcg ccgctttgct caggatgact tgggatcaaa gcgccgcctc ctgcgccacg
     1201 atcgcatcga acacttcctg cggcgttttc gccagccgta gcgcctgctg aaacttatcg
     1261 atcgtcatca accggctgat ccgcgccagc gcgtggatgt gcgggccggt tttttccggc
     1321 gggctggcca gcatccagat caccgacacc ggccggccat cgatcgattg aaaatcgatc
     1381 ggctgcgccg gtttgccgat cgccatcacc aggtccttcg tgccggggca tttgccgtgc
     1441 ggaatggcaa ggccgttgcc gatgccggtg gtgcgcgtcg cttcgcgttc cagcacggcg
     1501 tcgaggactt tcttcggatc ggtcacttgt ccgctggccg cgaggacctg caccagctcg
     1561 gcgatggcgt cgttcttggt tttcgcttcc agggggactt tgatgttttc cggtttcagg
     1621 atgtccgtca gccgcatgat tcaaaagctc cggcccgatt gtcattcagt ctgcaacacc
     1681 gcgcgcattt cggctcgcaa caagcgaaag caaaatcgta ccgcgacggg cgcggtgtgg
     1741 caaatgccgg ttaacgcaag tgacgcttcg ccattcttct tccatgcgta gcggtagccc
     1801 cgaaggggca cgtgttttca cgtagcacgg cgcctctggc ccgtgatgcg tgtactcacg
     1861 cacgctcgct gtacggatcg acacggcaat cgcctccgcg accgttgtcg aaagctcgtc
     1921 gctgacgcga ttgcttcgtc gacggagtac cgccgacatt cgtggagtac cacgaaacac
     1981 aggcaagatg cccgtgccac gtgaaaacac gcgcccctgc ggggctaccc gctacgcatg
     2041 ggaattgaag tcacgcgatc gtcgcgacct attgaagtga cgccggtttc gccgagtcgc
     2101 aggtggagca tcccgcctcg gcgcacggct cgacgtgcgc tgtcgcgttt ccctcgccca
     2161 gcgcctgctc gatctcgtat tcgatggcgc tggccgcgcg gtggccctgc tcgatgtccc
     2221 agtaggccgg caccatcatg tggaaatcga cccagtgata tcgcccgctg tggcgatggc
     2281 ggaccttgtg atagctgcag atccgcggct cgctgccgcg ttcgccgacg tgcgaatcga
     2341 gaatccgccg cagcatcgcc gcgtcatcca cgtcctgctg gtccatcaaa cccgcagcgg
     2401 agcgcctgag cagcccgatg ccggtgtatg cgatgtagaa cgccgccccg atcgccgcga
     2461 tcggatcgat gcgcgtccag ccactgatgc gcaccaacac caatgccacg agcgcgacgg
     2521 agctggtcac ggcgtcggta atgagatgcc ggccgtctgc ttccagcgtg atggacgaca
     2581 gccgcttgcc ggtgcgaatc aggaaccacc caacggcggc gttcacgacg agcgcgatga
     2641 ccatcagcac cacgccgacg ccgagcgcct ccatgtgcag cggcaagccg cggatcagcg
     2701 tgtccagggc tttgccgatg gcgatggcgg ccgccagcag gatcatcccg ccttcgaacc
     2761 cggcgga
//
LOCUS       DATIMH010000387         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1826077, whole genome shotgun sequence.
ACCESSION   DATIMH010000387 DATIMH010000000
VERSION     DATIMH010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826077"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VLI90_06705"
     CDS             <1..498
                     /locus_tag="VLI90_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13932.1"
                     /translation="LLATSVSDALPLFAGHAAIQSRLATLESVGLGYLTLGQPLNTLS
                     GGESQRLKLVHYLSGFAADRKTDAGVAKQAGALLLLDEPTTGLHFDDVRKLLDVLQRL
                     TDLGNTVVVIEHNLDVMKAADWIIDLGPDGGEYGGRIVASGTPEQVARHKKSYTAEAL
                     RKVMP"
     gene            495..1451
                     /locus_tag="VLI90_06710"
     CDS             495..1451
                     /locus_tag="VLI90_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13933.1"
                     /translation="MSRYAQRPIDLSGLKTIGLRERGGKVKVGDFAAPYAQGSGVAGL
                     VASLPQVLAAEHFRAVVDAIVAAKVQGRAILWGLGGHVIKCGLAPVLIDLMRRGLATG
                     FALNGAAAIHDFEIAMAGHTSEDVEAVLPGGRFGTAEETGREMNRAIVEGDRDGLGMG
                     EALGRALSGLSVACPEASLLLNAYGAGVPVTVHVAIGTDTPHTYPAADGAAIGSTTLR
                     DFRLFCSLATELDGGGIYLNVGSAVLLPEVFLKAVSTVRNLGYPLTEFTTANFDFLQH
                     YRPRANVVERPHAEAGGKGYAITGHHELMIPLLAAALIEKTA"
     gene            1589..2230
                     /locus_tag="VLI90_06715"
     CDS             1589..2230
                     /locus_tag="VLI90_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HSV13934.1"
                     /translation="MLLGFGTVKLALWMLRLHPSVNTWELLAQQFAGYGFLFGALAVL
                     FRMQYDRPLWRSLGWVTPRIPLYRIAWYGVGLAMCLGVLGKLIRLPMVDNPLTKLLKD
                     PTSLILIAVFGSIVAPVCEELLFRGFLQPLLVRSLGAFMGVLAAALPFGLLHYQEYGN
                     SWRHVLLITLSGVAFGWMRQATGSTKAAAGLHAAFNGFQFVALIAAKGLRPDL"
BASE COUNT          374 a          640 c          930 g          500 t
ORIGIN      
        1 ctgctcgcca cgagcgtgtc cgatgcgctg ccgctgtttg ccgggcacgc cgcaatccag
       61 tcgcggctcg cgacgttgga atcggttggg ctcggctatc tcacgcttgg tcagccgctg
      121 aatacgctga gcggcggcga atcgcagcga ctgaagctgg tgcattacct cagcggtttt
      181 gccgccgacc gcaaaaccga cgctggcgtc gcgaagcaag cgggtgcgct gctgctcctc
      241 gatgagccga cgacgggttt gcacttcgac gacgtccgca agctgttgga tgtgctgcag
      301 cggctgacag atttgggcaa cacggtggtg gtgattgagc ataatctgga tgtgatgaag
      361 gcggcggact ggatcatcga tttggggccg gacggcggcg agtatggcgg gcggatcgtc
      421 gcgtcgggga cgccggaaca ggtggcgcgg cataagaaaa gctacacggc cgaggctctg
      481 cgcaaggtga tgccgtgagc cggtacgcgc agcggccgat cgatctgtcg gggttgaaga
      541 cgatcgggct gcgggagcgc ggcggcaagg tgaaggtggg cgatttcgcg gcgccgtacg
      601 cgcaggggtc gggcgtcgcg ggcctggtgg cgtcgctgcc gcaggtgcta gcggcggaac
      661 attttcgcgc ggtcgtggat gcgatcgtcg cggcgaaggt ccaggggcgc gcgattctgt
      721 ggggcttggg cgggcatgtg atcaagtgcg ggctggcgcc ggtactgatc gacttgatgc
      781 ggcgcggtct ggcgacgggc ttcgcgttga atggggcggc ggcaatccac gacttcgaaa
      841 tcgcgatggc ggggcatacc agcgaggatg tggaggccgt cctgcccggc ggccgtttcg
      901 gcacggcgga agagacggga cgcgagatga atcgcgcgat cgtcgagggc gatcgcgatg
      961 ggctggggat gggcgaggct ttggggaggg cgctgtcggg tttgagcgtg gcgtgccccg
     1021 aggcgagtct gttgttaaat gcgtatggcg cgggcgtgcc ggtcacggta cacgtggcga
     1081 tcgggacgga tactccgcac acgtatccgg cggcggacgg cgccgcaatc ggcagcacga
     1141 cgcttcggga cttccggctg ttctgcagtt tggcgacgga gttggatggc ggcgggattt
     1201 atctgaatgt cgggtcggcg gtgctgctgc ccgaagtgtt cttgaaggct gtgtcgacag
     1261 tgcggaacct ggggtatccg ttgacggagt tcacgacggc gaacttcgac tttctgcagc
     1321 actacaggcc gcgggctaac gtggtggagc ggcctcatgc ggaagcgggc ggcaagggat
     1381 acgccatcac agggcatcac gaattgatga ttccgctgct ggcggcggcg ctcatcgaga
     1441 agactgcatg atggacgagc ccgggcccga gcaacgggag gaggttgcgc ctttagatgg
     1501 cgcgccgcct gagcctgggc ctcccgagcg tgttcctttc tggggctata cggatcttct
     1561 gattttttgc gggctggcga ttccctgcat gctgctgggt ttcggtacgg tgaagctcgc
     1621 gctgtggatg ctacggctgc atccctcggt gaacacgtgg gaattgctcg cgcagcagtt
     1681 cgcgggctac ggatttttgt tcggcgcgct ggccgtgttg ttccgaatgc agtacgaccg
     1741 gccgttgtgg cgatcgctgg ggtgggtgac gccgcggatt cctttgtacc ggattgcgtg
     1801 gtatggcgtg gggctcgcga tgtgcctggg cgttttgggc aagctgattc ggttgccgat
     1861 ggtcgataac ccgttgacga aactgctgaa ggatccgacg tcgttgattc tgatcgcggt
     1921 gtttgggagt attgtcgcgc cggtgtgtga agagttgctg ttccgcgggt tcctgcagcc
     1981 gctgctggtg cgcagtctgg gggcgttcat gggcgtgctt gccgcggcgc taccgttcgg
     2041 gctactgcac taccaggagt atgggaattc gtggcggcac gtcctgctga ttacgctctc
     2101 gggcgtggcg ttcggatgga tgcggcaggc gacggggtcg acgaaggccg ccgcgggatt
     2161 gcacgccgcg ttcaatggat tccaattcgt ggcgctgatt gcggccaagg ggctgcggcc
     2221 ggatttgtag caggcgccga gcgcctatcg aactcggctc gacactccct tacggtcgtc
     2281 gctccgacgg gggcgcgact ttccaactct aacctcggag ctgcgctccg atgcgggacg
     2341 agggcgtccc gcgcggacca gggggtccgc cccacagacg aaatcttggg atggtacctg
     2401 taaccattaa gatagggtga attttgttgg ggcggtgata tggt
//
LOCUS       DATIMH010000388         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5291527, whole genome shotgun sequence.
ACCESSION   DATIMH010000388 DATIMH010000000
VERSION     DATIMH010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5291527"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..603)
                     /gene="grpE"
                     /locus_tag="VLI90_06720"
     CDS             complement(43..603)
                     /gene="grpE"
                     /locus_tag="VLI90_06720"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSV13935.1"
                     /translation="MSRIPIDDDNFEPDAQLDEDTASQQPSGGGGQPDSNEVIKLRTE
                     RDQLFERLARATAEFKNSQKRLESEFDQRVQYANSSLIRSLLPVIDNFERALSQDATK
                     ADAATILKGMQIVHDQLLAVLKQQKVEEIAPKAGETFDPTRHEALMHQPSDQYTEPTV
                     TQLLQKGYALQGRTLRPAQVAVSKMG"
     gene            complement(690..1829)
                     /gene="dnaJ"
                     /locus_tag="VLI90_06725"
     CDS             complement(690..1829)
                     /gene="dnaJ"
                     /locus_tag="VLI90_06725"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HSV13936.1"
                     /translation="MAVAKRDYYEVLGVARTASGDEIKRAYRRLAMKFHPDRAEADGK
                     HEAEAKFKECSEAYEVLSDEIKRQRYDQYGHAGVSASHDYSHMEAADIFSMFEDIFGG
                     FGGGGGRGRGASRGATRGFDLETQVELTLAEVASGTEKTIEFEKQDVCDTCKGSGSKP
                     GSSPVVCVQCGGQGRIAQQGFGGMFRMVTTCPNCRGRGTVIRDHCPSCGGTGRQLKKR
                     VVTVKIPAGVHEGQAVRIVGEGEAGENGARPGDLHCYITVKPHPIFTRHNNDLVCQVP
                     ISFTQAALGSQIDIPTLAGSEKLDIPAGSQHGEVFKLKGKGLPDIRAHRTGDELVQIL
                     IEIPRKLNDRQRELLREFAGTEDGNVMPQRKGFMDKLKEAIKGKD"
     gene            complement(1876..2169)
                     /locus_tag="VLI90_06730"
     CDS             complement(1876..2169)
                     /locus_tag="VLI90_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13937.1"
                     /translation="MFLEGVIHQLGAFALAMNVQPDDIHVRLTLDDGDAVVVRAFKTV
                     SAFAGSEWGMILGVGKNSEEALVLRESHILLAHFQLAPEGPASIGFGTESKAG"
BASE COUNT          407 a          740 c          652 g          498 t
ORIGIN      
        1 aaggatgaat gcagaatggc ggcgagcggc ttcacgcgcg cgtcacccca ttttagagac
       61 cgccacctgc gccggccgca gcgtgcggcc ttgcagggcg tagcctttct gcaacaactg
      121 cgtcactgtc ggttccgtgt attgatcgct cggctgatgc atcagcgctt catggcgcgt
      181 cggatcgaac gtctcgccgg ccttgggcgc gatctcttcc accttctgct gcttcagcac
      241 cgccagcagc tgatcgtgaa cgatctgcat gcccttgagg atcgtcgccg cgtcggcttt
      301 ggtggcatcc tgtgacaggg cgcgctcgaa attatcgatc accggcagca gactgcggat
      361 gagcgatgag ttggcgtact gcacgcgctg gtcgaattcg ctttccagcc gcttctggct
      421 gttcttgaat tcggccgtcg cgcgggcgag gcgctcgaac agctgatcgc gctcggtgcg
      481 cagcttgatc acttcgttcg aatcaggctg accaccacca ccggaaggtt gctggcttgc
      541 cgtgtcctca tccagctgag cgtcgggctc gaagttgtcg tcgtcgattg gaatgcgact
      601 cattttgttt gtccgttgtc cgttctccgg cgtcagtcgt gcaagcatat tctgccacaa
      661 ctgaccacag accacggacc actgactcct caatctttgc ctttgatggc ttccttcagc
      721 ttgtccatga atcctttgcg ctgcggcatc acgttgccgt cttcggtgcc ggcgaactcg
      781 cgcagcaact cgcgctggcg gtcgttcagc ttgcgcggga tctcaatcag tatttgtacg
      841 agttcatccc ccgtcctgtg agcgcgaata tccggcaggc ctttgccttt gagcttgaag
      901 acttcgccgt gctggctgcc ggcggggatg tcgagctttt ccgagccagc cagcgtgggg
      961 atatcaatct gcgagccaag cgccgcctgc gtgaagctga tcggcacctg gcagaccaga
     1021 tcgttgttgt gccgggtgaa aatcggatgc ggcttcaccg tgatgtagca atgcagatcg
     1081 cccggtcgag cgccgttctc gcccgcttca ccttcgccga cgatgcggac cgcttgtcct
     1141 tcgtgaacac ccgcggggat tttgacggtg acgacgcgct tcttcagctg ccgtccggtg
     1201 ccgccgcaag atgggcaatg atcgcggatg accgtgccgc gcccgcggca gttggggcag
     1261 gtggtgacca tgcggaacat gccgccgaac ccctgctgcg cgattcgacc ttgtccgccg
     1321 cattgaacgc agacgaccgg cgaactaccg ggtttgctgc cgctgccttt acaggtgtca
     1381 caaacatcct gcttctcaaa ttcaatcgtc ttctcagtac cgctggccac ctcagccaat
     1441 gtcagttcga cctgcgtctc cagatcaaac ccgcgcgttg ccccgcgaga cgcgccgcgg
     1501 ccgcgccccc caccgccgcc gaagccgcca aaaatatcct cgaacatcga gaagatgtcc
     1561 gcggcttcca tgtgcgaata gtcgtgcgag gcgctgacgc cagcgtggcc gtattggtcg
     1621 tagcgctgcc gcttgatctc gtcggagaga acttcgtacg cctcggaaca ctctttgaac
     1681 ttcgcctcgg cttcgtgctt gccatccgcc tccgcccggt ccggatgaaa cttcatcgcc
     1741 agccggcgat aagcgcgctt gatctcgtca ccgctggcgg tgcgggcgac gccgaggact
     1801 tcgtaatagt cacgtttggc tacggccact atagtgctcg aaaaagatga ccttcagcgc
     1861 acgcgccggc atcacctagc ctgccttcga ctccgtgccg aatccaatgg acgccggacc
     1921 ttctggtgcc agttggaaat gagcgagcag gatgtgcgac tctcgaagca caagcgcctc
     1981 ctccgaattc tttccaacac ctaggatcat gccccactcg cttccggcaa acgccgacac
     2041 cgtcttgaag gcacgaacca cgactgcgtc gccgtcatca agcgtcagtc gaacgtgaat
     2101 gtcgtctggc tgaacattca tcgccaaggc aaacgcgccg agttggtgaa tcacaccttc
     2161 caggaacatc cgatgattcc attaagcggc cgaggcttcc gctgctgctt ggtggcgacc
     2221 ggctaccgtc aggcgatgta gtcccttggg cactccgaaa tagctgatta cgacaccagc
     2281 cggctccatc aaccccg
//
LOCUS       DATIMH010000389         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2400370, whole genome shotgun sequence.
ACCESSION   DATIMH010000389 DATIMH010000000
VERSION     DATIMH010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2400370"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..>1532
                     /locus_tag="VLI90_06735"
     CDS             66..>1532
                     /locus_tag="VLI90_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HSV13938.1"
                     /translation="MPVSKYLAAVIVSIIAPLAMRARADVTPASPFTDHMVLQRDMPV
                     PVWGTAPPGEHVTVTIGTSTKGATAGEDMRWMLRLPPLNAGGPYEMTITGSGGSVIAL
                     KDVLIGEVWLCSGQSNMDFTVARMPKYYFAGTRNEAEEVAAADYPQIRMFSGHWRRSY
                     TPQVAVPGEWRVCTPQNVREFSAVGYFFARDLHKALNVPVGIVTETYGASTAEAWISR
                     EALAADPQLKPILDGFDAKATTYPSEQRVKDLEALGHWEAEMAKLDPTTRPIKAPPNH
                     DPANDQHNPTVLFNGMIAPIIPYAIRGVLWYQGESIVGGANGVKLYPLVQRTLVNDWR
                     QRWGEGDFPFYIVQLAAYKAPATQPVQNSGIANVRAAQQTILSLPNTGMAITIDIGDA
                     KNIHPKDKQDVGDRLTRIALAKTYGQPIEYSGPIYESMAVEGNTIRVKFTHTTGGLVA
                     RGGPLKQFAIAGKDGQFVWADAKIDGDSVIVSSDRVSDP"
BASE COUNT          312 a          470 c          506 g          244 t
ORIGIN      
        1 tcgtcggcag atattcgagg cacggaccag gtgcagatca cgcactgaga acgagggaga
       61 agatcatgcc cgtctcgaaa tatctcgcgg ccgtcatcgt gtcgatcatc gcgccgcttg
      121 cgatgcgggc acgcgccgac gtgacgccgg ccagtccgtt cacggatcac atggtgctgc
      181 agcgggacat gccggtgccg gtgtggggca ccgcgccgcc gggcgagcat gtgaccgtga
      241 cgatcggcac cagcaccaag ggcgcgacgg ccggcgaaga catgcgttgg atgctgcggc
      301 tgccaccgct gaacgcgggc gggccgtatg agatgacgat caccggttcg ggcgggtcgg
      361 tcattgcgct gaaggatgtg ctgatcggcg aagtctggct ctgctcgggc caatccaaca
      421 tggacttcac agttgcgcgc atgcccaaat attatttcgc gggaaccagg aacgaagccg
      481 aagaggtggc ggcggctgat tacccgcaga ttcgaatgtt cagcggtcat tggcggcgga
      541 gttacacgcc gcaggtcgcc gtgcccgggg aatggcgggt gtgcacgccg cagaacgtgc
      601 gtgagttttc tgccgtcggt tatttctttg cgcgcgatct gcacaaggcg ctgaacgtgc
      661 cggtggggat cgtcaccgag acgtacgggg cgagcaccgc tgaagcgtgg atcagccgcg
      721 aggcgttggc cgcggatccg caacttaagc ccatcctcga cggcttcgac gccaaggcga
      781 ccacctatcc cagcgagcag cgcgtcaaag accttgaggc gctggggcat tgggaagcgg
      841 agatggccaa gctcgatcca acgactcgcc cgatcaaggc gccgccgaac cacgacccag
      901 cgaacgacca gcacaatccg acggtgttgt tcaacggcat gatcgcgccg atcattccgt
      961 acgccatccg cggggtgctg tggtaccagg gcgaatccat cgtcggcggc gccaacggtg
     1021 tcaagctcta cccgctggtg cagcgcacgc tcgtcaacga ctggcggcag cgttggggtg
     1081 aaggggactt tccgttctac atcgtgcagc tggccgcgta caaagcgccg gccacccagc
     1141 cggtgcagaa cagcggcatc gccaacgtgc gtgctgcgca gcagacaatc ctgtcgctgc
     1201 cgaacaccgg catggcgatc acgatcgaca tcggcgacgc caagaacatt catcccaagg
     1261 acaagcagga cgtcggcgac cgcctgacgc gcatcgcgct ggcaaaaacg tatggccagc
     1321 cgatcgaata ttcgggcccg atctacgaat cgatggcggt cgaaggcaac acaatccgcg
     1381 tgaagttcac gcacaccaca ggcggactcg tggcgcgcgg tggaccactc aaacaattcg
     1441 cgatcgccgg caaggacggc cagttcgtct gggctgacgc gaagatcgac ggcgacagcg
     1501 tgatcgtcag cagtgatcgc gtgagcgacc cc
//
LOCUS       DATIMH010000390         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_958404, whole genome shotgun sequence.
ACCESSION   DATIMH010000390 DATIMH010000000
VERSION     DATIMH010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958404"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2944)
                     /locus_tag="VLI90_06740"
     CDS             complement(<1..2944)
                     /locus_tag="VLI90_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV13939.1"
                     /translation="MTQTAVPVISPDDDAALLREFAQTHSQAAFRQIVDRYVNMVHAA
                     ARRQVRDPHLAEDITQAVFLLLSQRAGVVSAQRAVGGWLLLTTRNVARNALKREWRLK
                     RREQEAAKMNSEERLASSPEAATIAPMLDDALARLAEADRNAIFARFFNGGSHRDVGR
                     QLGVTEEAATKRVSRAVGKLRAILERQGITTTEASLSALLPVIALKPAASVALVSSVV
                     GTASAAAPPAAIAALAHGAAAGSTLSGTSIAVGAGLVAVIAAGAFLAVATLMNKPPAP
                     PVAAAPLPANPAPVAPPARRAIAAAPAPAGRTVTLSMVDAKTAAPIPDVQVQLELENG
                     PQTTARADANGKYVLNLPATFQYARAFCSAPGHAAMSIDFPNYTFKGQLLADYTVPME
                     AGTKIGGIVVDESGEPIIGATVSLSSYIRGPMDQPRPILRGENVQTGVDGRWSFDNAP
                     SDTSLINIEISHPDYPKNQPYSDAPKDALLRGDYKRELKWQVGRSLDGVVFDPDGKPL
                     AGVTLVVASDRYESSKPKATSDAAGKFHLTGITDRYRTFVTATAPGFAPQQVELTAAG
                     PDTKPTDPLKPIEVHLEKGVMLEGAVVDAAGKPLIGAKVEVDKWRGNRALVWSTKTDR
                     QGHFAWPNAPTDPMEFIASKKNFAALQGAEVKAGNGPVTLTLHPPLHITGNVIDAETK
                     QPIPAFRVVYGIRWTGQDDVIWQSQDARSISNGKYEAHITNFSNGGRLRVEADGYLPE
                     QSRLIEAGEGTISIDFELKRGNGPSGTVVDAGGKPVPNLRVVGIPDNAGIYIDWTNGS
                     LDDQERLAVTRTDKDGHYRLRAMETHYSLLAVGDGGFVALKQDDVPRDGTLKLLQWAR
                     FEGDLRLNAGPLVNATIAAQTTSLNGMNCSAMPGQATTDAAGHFVIDRVVPGQPIMLY
                     QLVPMGGGSSRYRVIGRYSSVAGQTIKVRAGGVGRPVIAKLLPPPGMKDPVNWPQQLS
                     IEPVVSSYT"
BASE COUNT          504 a         1010 c         1059 g          603 t
ORIGIN      
        1 cggtgtacga cgagacgacg ggctcaatcg acagctgctg cggccagttc accgggtcct
       61 tcatgcccgg cgggggcagg agcttggcga tgaccggccg gcctacgccg ccggcgcgga
      121 ccttgatggt ctgaccggcg acgctgctat accggccgat cacgcgatag cgcgacgacc
      181 ccccgcccat cggcaccagt tgatacagca tgatcggctg gccggggacg acgcggtcga
      241 tcacgaaatg ccccgccgcg tcggtcgtcg cttgtccggg catcgcgctg cagttcatgc
      301 cattcaggct tgtggtctgc gcggcgatgg tcgcgttcac cagcgggccg gcgttcaggc
      361 gcaggtcgcc ttcgaagcgc gcccactgca gcaacttgag ggttccgtcc ctaggcacat
      421 cgtcctgctt gagcgcgacg aaaccaccgt cgccgacagc cagcagcgaa taatgcgtct
      481 ccatcgcgcg aaggcgatag tgcccgtctt tgtcggtgcg cgtcaccgcc aggcgctcct
      541 ggtcgtcgag gctgccgttg gtccagtcga tgtaaatgcc ggcgttgtcg gggatgccga
      601 cgacgcggag gttgggcacc ggttttccgc cggcatcaac gacggtgccg ctgggaccgt
      661 tgccgcgttt caactcgaag tcgatgctga tcgttccctc gccggcttcg atcaggcgcg
      721 actgctcggg cagataacca tccgcctcga cgcgcaaccg gccaccgttg gagaaattgg
      781 tgatgtgcgc ttcgtacttg ccattgctga tcgaccgtgc gtcctgcgac tgccagatca
      841 catcgtcctg cccggtccaa cgaatgccgt acaccacgcg aaaggctggg atcggctgct
      901 tggtctcggc gtcgatcacg ttgccggtga tgtgcagcgg cggatgcagc gtcagcgtca
      961 ccgggccgtt gccggccttc acctccgcgc cctggagggc ggcaaagttc ttcttgctgg
     1021 cgatgaactc catcggatcg gtcggcgcat taggccaggc gaaatggcct tgccggtccg
     1081 tcttggtgga ccagaccaac gcgcgattcc ctcgccactt gtccacctcc accttcgcgc
     1141 caatgagagg tttgcccgcg gcatccacca cggcgccttc gagcatcacg cctttttcca
     1201 ggtgcacttc gatgggcttc aagggatcgg tcggcttggt gtcaggcccc gcggcggtta
     1261 gctcgacctg ctgcggcgca aagcccggcg cggttgcggt gacgaacgtg cgataccgat
     1321 cggtgatgcc ggtgaggtgg aacttgcccg ccgcgtcgct ggtggcctta ggtttgcttg
     1381 attcataacg atcgctggcg acgaccagcg tcacaccagc cagcggtttg ccgtcgggat
     1441 cgaacacgac accgtcgagc gaccggccga cctgccactt cagctcgcgc ttgtaatcgc
     1501 cgcggagcag cgcgtccttg ggcgcgtcgg agtatggctg gttcttcgga taatccgggt
     1561 gcgagatttc gatgttgatc aggctcgtgt cgctgggcgc attgtcgaat gaccatcggc
     1621 catcgacgcc ggtctgcaca ttttcgccgc ggaggatcgg gcgcggttgg tccatcgggc
     1681 cgcggatgta cgacgagagg ctgactgtcg caccgatgat cggttcgccc gattcatcaa
     1741 cgacgatgcc gccgatcttc gtgccggctt ccatcggcac ggtgtagtcc gccagcagtt
     1801 ggcctttgaa ggtgtagttg gggaagtcga tgctcatggc cgcatggcct ggcgcgctgc
     1861 agaaggcgcg agcatattgg aatgtcgcgg ggaggttcag gacgtatttg ccgttggcat
     1921 cggcgcgggc agtcgtctgc ggcccgttct cgagttcaag ttgcacctgc acatccggga
     1981 tgggcgcggc ggtcttggca tcgaccatcg acaaggtgac ggttcgaccg gccggcgctg
     2041 gcgcggcggc gatggctcga cgcgcgggcg gcgcaaccgg cgcaggattg gccggcagcg
     2101 gcgcggcggc gacgggcggc gcgggcggtt tgttcatcag cgtcgccacc gcaaggaatg
     2161 ctcccgcggc gatgaccgca accaacccag cgccgacggc gatgctggtg ccagacagcg
     2221 tcgatcccgc cgccgcaccg tgcgccagcg ccgcgatcgc cgcgggtggc gcggcggcgg
     2281 atgcggttcc cacgacgctc gacacgagcg cgacggatgc cgcgggcttc agcgcgatca
     2341 ccggtaacag tgccgacagg cttgcttcag tggtagtaat gccttggcgt tccaatatcg
     2401 cgcgcagctt gcccaccgcg cgcgagacgc gcttggtcgc cgcttcttcg gtgactccca
     2461 gttgtcttcc gacatcgcga tggctgccgc cgttgaagaa gcgcgcgaag atcgcattgc
     2521 gatccgcctc cgccagtcgg gccagcgcgt cgtcgagcat cggcgcgatg gtggccgcct
     2581 cgggtgagga cgcgaggcgt tcttcgctgt tcatcttcgc cgcctcttgt tcgcggcgtt
     2641 tgagccgcca ttcccgcttc agcgcgttgc gcgcaacatt gcgcgtcgtc agcagcaacc
     2701 agccgccgac ggcgcgctgc gcgctgacga cgccggcgcg ctgcgacagc aacaggaaca
     2761 ccgcctgggt aatatcctcg gccaggtggg gatcgcgcac ctgccgccgg gcggcggcgt
     2821 gcaccatgtt gacgtagcgg tccacgatct gccgaaaggc ggcctgcgaa tgggtctgag
     2881 caaattcgcg cagcaatgcc gcgtcgtcgt cgggggaaat gactggtact gcggtctgag
     2941 tcatcagctt caaatgtgcg ccgcgcaaga tgatcggaca agcggcgact atccgatatt
     3001 acggcaggcc atattacgca aaggctgcac gaaaggatca cgccgcggga aacctttttc
     3061 cgatcctcgt caatttgcga ttgacatata cccggcgcgt ggcagaatta gcgtacagag
     3121 ctattccggt cgcggggctg ttcgttcctt cctgcagcgc gatttgaata gtgggc
//
LOCUS       DATIMH010000391         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2400860, whole genome shotgun sequence.
ACCESSION   DATIMH010000391 DATIMH010000000
VERSION     DATIMH010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2400860"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VLI90_06745"
     CDS             <1..396
                     /locus_tag="VLI90_06745"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HSV13940.1"
                     /translation="GHIVPTAIDSHGVFYVGNLNTFPIVPGSSKILQITPSGQVHTVA
                     TGLTTVLGVAFDHRAGMYALEMTATAGFPAPGAGALVRITGGKKETLVSGLDFPTAMT
                     FGPDGALYVSAFGLGPPGAGEVLRIRIDD"
     gene            606..1028
                     /locus_tag="VLI90_06750"
     CDS             606..1028
                     /locus_tag="VLI90_06750"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HSV13941.1"
                     /translation="MRKLILLAALVATALPSAAIAQRHDRHDRREDRREVRHDRREVR
                     RDRHELRHDRRDVRRDRSHRRVAYAAPYRNWRYRPVTVGYRLQPAFYGSRYYINDYGR
                     YNLRAPHRWERWIRYGDDLLLVNLRTGRVLRVIHYRYW"
     gene            complement(1041..>1857)
                     /locus_tag="VLI90_06755"
     CDS             complement(1041..>1857)
                     /locus_tag="VLI90_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HSV13942.1"
                     /translation="ATLKAIIAAIDIPLDSVILETQMVELTESGQKALGIDFANAAGQ
                     IGVVNVSSGAFNPVGVTQGCNGDLVNCSGHVTSAQFQAALYAQIQKGNGRIISTPRIA
                     AQSGSTAKIITGDALPILTSIALSGVNGVSQQVQYVNVGVTLQIAPRVSGDGFVSSHV
                     FVVVSSVTGFSQGYPTISQREAQTSATVRDGDSFVIGGLTQDANISTKTKVPLLGDLP
                     LVGQAFRSDRYTKARTELYIVVVPHIVHHVGARTLPAPNAPLYAPMTTTIRSQ"
BASE COUNT          326 a          577 c          607 g          347 t
ORIGIN      
        1 ggccacatcg tcccgactgc gatcgacagc cacggagtct tctacgtcgg caacctcaat
       61 actttcccga tcgtgccggg ttcatcgaag attcttcaga tcaccccaag cgggcaggtc
      121 cacaccgtcg cgactggctt gacgacagtg ctgggagtcg cgttcgacca tcgggcgggg
      181 atgtacgcgc tggagatgac cgccactgcc ggtttcccgg cgccgggcgc tggcgcgctc
      241 gtgcgcatca cgggagggaa aaaggaaacc ctcgtcagcg gactggattt cccgaccgcg
      301 atgacgttcg gccccgacgg agcgctctac gtttcggcgt tcggccttgg accgcccgga
      361 gcgggagagg ttctccgcat ccgaatcgac gactgagcca cgcgtccgcc gagggagccg
      421 ggccaaatcc gcgaccgcgg atcgcgcggc gaccgtggcc cttggcgcga gctgaaggcg
      481 gcagcggcaa cgcgacgaag gcccgtcagc gatggcgggc cttttcacgt ctttcgggat
      541 cgtgactggg cgcgtttggt ctcgttcatg ccgggcgtgc aacaagcgac gggaaggaga
      601 atgagatgag aaagttgatc ctcttggcag cactggtggc gacggccctc ccgagcgccg
      661 cgatcgcgca gcgccatgac cgccatgacc gccgtgaaga ccgccgcgaa gtccgtcacg
      721 accgtcgcga agtgcggcgc gaccggcatg agttgcgcca cgatcgccgc gacgtccgcc
      781 gcgaccgcag tcaccgccgc gtcgcttatg ccgcgcccta tcgcaactgg cgctatcggc
      841 cggtgaccgt cggctatcgg ttgcagccgg cattctatgg atcgcgttat tacatcaacg
      901 actacggcag gtataatctg cgtgcgcccc atcgttggga acgttggatc cgctacggcg
      961 acgacctgct gctggtgaat cttcgcaccg ggcgcgtact gcgggtgatc cattaccgct
     1021 attggtaatc gctggatggc tcattgcgat ctgatcgtgg tcgtcatggg cgcatagagc
     1081 ggcgcattgg gggcggggag ggttctggct ccgacgtggt gcacgatatg cggtaccaca
     1141 acgatgtaca gctccgtcct cgccttcgtg tagcgatcgc tccggaaggc ctgcccgacc
     1201 agcggaagat cgccgagcag cggcactttg gttttggtgc tgatgttcgc gtcttgagtg
     1261 aggccgccga tgacgaagct gtctccgtcg cgcaccgtcg ccgaggtttg cgcctcgcgc
     1321 tggctgatgg tcggataacc ctggctgaag cccgtgacgc tggagacgac gacgaacacg
     1381 tgacttgaaa cgaagccgtc gccgctgacc cgcggtgcga tctgcaaagt cacaccgaca
     1441 ttcacatatt ggacttgctg cgagacgcca ttgaccccgg acaaggcgat cgacgtcaaa
     1501 atcggcagcg cgtcgccggt gatgatcttg gcggtcgaac cgctctgcgc cgcgatccga
     1561 ggagtggaaa tgatgcggcc attgcccttc tggatctggg cgtaaagcgc cgcctgaaac
     1621 tgggcgctgg tcacgtgccc cgaacagttc accaaatcgc cgttgcaccc ttgggttacg
     1681 ccgacgggat tgaacgcgcc tgagctcacg ttgacgacgc cgatctggcc tgcggcgttg
     1741 gcgaaatcga tgccgagcgc cttctgaccg ctttcggtca gctcgaccat ctgcgtttcg
     1801 aggatgacgc tgtcgagcgg aatatcgatc gcggcgataa tcgccttcag ggtggcg
//
LOCUS       DATIMH010000392         5604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1250011, whole genome shotgun sequence.
ACCESSION   DATIMH010000392 DATIMH010000000
VERSION     DATIMH010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250011"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VLI90_06760"
     CDS             <1..688
                     /locus_tag="VLI90_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13943.1"
                     /translation="NVATALAKTAKTVLIDLDLMRGGVAAAFDIRPRYTIADVMDTAD
                     KVDKQLLDNALTVHTASGLAVLSRPEMPEETQRVNQPGLHRLLGVLSRVYDYLVIDSM
                     MSISPLYNTVLQASDVNVIVMQLNVPSAKNAERFVGALRRMGVESSKMKLLANRYVKK
                     GWDIEPAEVERALGLSLSWMVPNDFKNAIAALNFGEPVVLRTPKCEMSQSLFTLATSL
                     TGKDTATKAA"
     gene            728..2197
                     /locus_tag="VLI90_06765"
     CDS             728..2197
                     /locus_tag="VLI90_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HSV13944.1"
                     /translation="MGLFTRNTPVEDQSIAAPKPITPAESQLIAPMPGTTIAPRAASA
                     PATLVAPRQSPVAPPQVSSERQVYFQQLRVRIHQKLVERLDVQNMRSLPPETVRNEVR
                     SLIRELCQSEKGLINGADQERLMDDVMDETFGLGPLESLLKDSTISDILVNKFDRIYC
                     ERRGRLELVDVRFRDNAHLRQIIERIVGRIGRRIDETSPMVDARLADGSRVNAIIPPL
                     ALDGPAMSIRRFGSKPMQLEDLIRHGAFPPAVMDFLSAAVQSRCNILISGGTGSGKTT
                     LLNCLSRYIPTDERVITIEDAAELQLQQPHVVRLETRPMNIEGKGEVTQRDLVKNCLR
                     MRPDRIIIGECRSGEAMDMLQAMNTGHDGSMTTVHANTTRDALGRLEVMIAMAGYDIP
                     IRALRTQISSAIQIVVQARRLTGGRRKVTSVSEITGMEGDSIAMHDLFTFEQAGVDDE
                     GHATGRFVATGIRPKAAERIEARGLKLPADLFARRVIDL"
     gene            2301..3266
                     /locus_tag="VLI90_06770"
     CDS             2301..3266
                     /locus_tag="VLI90_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSV13945.1"
                     /translation="MDGTILYILIAAVAALFGLGMVFLMQGLTDGDKRKLSQRLGSDD
                     RVDGNTATRRSIVIHQMESAGLPPALAKIQLLQSLHGRVIQAFPELPLARFLLIVSGL
                     AFVSFLISWVIADSLVAGLIAGAIAGYLPFFAMNSKRSKRQRQLADQIPDALDFLSRI
                     LRAGHSLTTGLQMMGEELRQPMAAEFRRCYDQHSLGQPLEDCLKDMATRVETTDFAFF
                     VTAVLIQRQTGGDLSEVLNNISGMIRQRIRLQQHVKAKTAEGRFTGYILVAFPAIMFL
                     IAWTMNPGYAGVLLHTSKGMMLLCTAFGLQMLGLWAIRKLTTVKV"
     gene            3506..4429
                     /locus_tag="VLI90_06775"
     CDS             3506..4429
                     /locus_tag="VLI90_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSV13946.1"
                     /translation="MDNEALIFLCVAGAITLVGFSLYLLFAPASNERLRSRLNGDAEQ
                     DATEAPGAAGVVPLLQKVGQAAAQPFMPKTREAQSDLRRRLGYAGYYSNSAIKMVTGA
                     KVICLAGGLGLGYLASTFTDTFMMQMAALSIGGLFGYLAPTFWLKFSIKANCKALTYG
                     LADALDLMVVCVEAGLTIDSAMQRVGQEMALAHPAISRELGICHMETRMGLSRMDALK
                     NLGVRTNCESLQSLSSMLIQADRFGTSIAAALRIHADTIRINRHHAAEELAAKASVKM
                     SFPLVLFIFPATFIVLAGPTVIQLMDSPLMK"
     gene            4512..5090
                     /locus_tag="VLI90_06780"
     CDS             4512..5090
                     /locus_tag="VLI90_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13947.1"
                     /translation="MANLHTHPTAKLTQLAALAITTLLLVAAGCHSNPNQKIAHGEEF
                     SDEKAATSVGQMAQAQTAAGAKNDGMLYDHNFHGTKLNSLGQTKLDLIVKGTPAGDPV
                     VVYLNVPQDGLAARRPAVLAYLHDGGLKDAQIKLIDGPATGENTPSAKTMSKIYMAKD
                     GSYTGEMAEDQAAGSAGGMGQPSSGGSPSASH"
     gene            complement(5171..>5604)
                     /locus_tag="VLI90_06785"
     CDS             complement(5171..>5604)
                     /locus_tag="VLI90_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13948.1"
                     /translation="FTEDRMRFRNEEERLVAEQALLNYRELRQVMKEAPHGKGLAVLE
                     QAVREKERTTAAYGPTSGRKELRECLQHQLRAPKARGFEVLELSGARMLARTPDAGFG
                     AYSRRPGFFWRAKEPRSSVYLRTRREARADPLRCSAKRMRR"
BASE COUNT         1089 a         1809 c         1744 g          962 t
ORIGIN      
        1 caacgtcgcc accgcgctgg ccaagaccgc caagactgtg ctgatcgacc tggacctgat
       61 gcgcggcggg gtggccgcag cattcgacat tcgcccgcgc tacacgatcg ccgacgtcat
      121 ggacaccgcc gacaaggtgg acaagcaact cctggacaac gcgctgaccg ttcacacagc
      181 cagcgggttg gccgtgctgt cgcgcccgga aatgccggaa gaaacgcagc gcgtgaacca
      241 gcctggtttg caccggctgc tgggtgtgct cagccgcgtg tacgactacc tcgtgattga
      301 ttcgatgatg agcatctcgc cgctctacaa cacggttctg caggcgtcgg acgtgaacgt
      361 gatcgtgatg cagctcaacg tgcccagcgc caagaacgcc gagcggttcg tcggcgcgct
      421 gcgccgcatg ggcgtcgagt ccagcaagat gaagctgctg gccaaccgat acgtgaagaa
      481 gggttgggat attgagcccg ccgaggtgga acgggcgctg ggcctgagct tgtcctggat
      541 ggtgccgaac gatttcaaga acgcgatcgc cgcgctgaac tttggcgagc cggtcgtgct
      601 gcgcacgcct aagtgcgaga tgagccagag cctgttcacg ctggcgacat cattgaccgg
      661 gaaggatacc gccaccaagg cggcgtgagc gagcaacgca taggtactga cgcgtaggta
      721 ctgacgaatg ggacttttta cccgcaatac tccggtcgag gatcagtcga tcgccgcgcc
      781 gaagccgatc acaccggcgg aatcgcagct gatcgcgccg atgcccggca cgacgatcgc
      841 gccgcgcgcc gcatcggcgc ccgctacgct ggtggcgccg cggcagtcac cggtggcgcc
      901 gccgcaggtg tccagcgagc ggcaggttta cttccagcag ctgcgcgtgc gcattcacca
      961 gaagctggtc gagcggctgg acgtgcagaa catgcgctcg ctgccgccgg aaacggtgcg
     1021 caacgaggtc cgctcgctca ttcgcgagct gtgccagagc gaaaagggcc tcatcaacgg
     1081 ggcggatcaa gaacggttga tggacgacgt catggacgag acgttcggcc tgggcccgct
     1141 cgaatcgctg ctgaaggact cgacgatcag cgacatcctc gtcaacaagt tcgaccgcat
     1201 ctattgtgaa cgccgtggcc ggcttgagtt ggtggacgtg cgcttccgcg acaacgcgca
     1261 cctgcggcag atcatcgagc ggatcgtcgg ccggatcggc cggcgcatcg acgagaccag
     1321 cccgatggtg gacgcgcgcc tggccgacgg cagccgcgtt aacgcgatca tcccgccgct
     1381 ggcgctcgat ggcccggcaa tgagcatccg ccgcttcggt tccaaaccga tgcagctgga
     1441 agacctgatc cgccacggcg cgtttccccc ggcggtgatg gatttcctct ccgccgcggt
     1501 gcaatcgcgc tgcaacatct tgatttccgg cggcaccggt tcgggcaaga cgacgctgct
     1561 gaactgcctc tcgcgctaca tcccgacgga cgagcgcgtg atcacgatcg aagacgccgc
     1621 cgaactgcag ctgcagcagc cgcacgtggt gcgcctggaa acgcggccga tgaacatcga
     1681 aggcaagggc gaagtcaccc agcgcgacct ggtgaagaac tgcctgcgta tgcggccgga
     1741 tcgcatcatc atcggcgaat gccgatcggg cgaagcgatg gacatgcttc aggcgatgaa
     1801 cacgggtcac gatggatcga tgaccaccgt tcacgccaac acgacgcgcg atgcgctcgg
     1861 tcgcctggaa gtgatgatcg cgatggccgg ctacgacatc ccgatccgcg cgctgcggac
     1921 gcagatttcc agcgcgattc agattgtcgt gcaggcccgc cggctgacgg gcggccgccg
     1981 aaaggtgacg agcgtcagcg agatcaccgg catggaaggc gacagcatcg cgatgcacga
     2041 cctgttcacg ttcgagcagg ccggcgtgga tgacgaaggc cacgccaccg gccggttcgt
     2101 cgccaccggc attcgaccca aggccgccga gcgcatcgaa gcgcgcggcc tgaagctgcc
     2161 ggcggacctg tttgcgcgga gggtgatcga cctgtgaggg gtcaggagtc aggggtcagg
     2221 aaagagaagg aaccttttcc cgacccctga cccttgaccc ttgacccttg acccttgacc
     2281 cctgacccct gacaaagact atggacggca caatcctcta catcctcatc gcggccgtcg
     2341 cggccttgtt cgggctcggt atggtcttcc tcatgcaggg cctgaccgac ggcgacaagc
     2401 gcaagctaag ccagcgcctc ggcagcgatg accgcgtcga tggcaacacc gccacgcggc
     2461 ggtcgatcgt cattcaccag atggaatccg ccggcctgcc gccggcgctg gcgaagatcc
     2521 agctgcttca aagcctgcac ggccgcgtca tccaggcgtt tccggaattg ccgctggcgc
     2581 gcttcctgct gatcgtcagc gggctggcgt tcgtcagctt tctcatcagc tgggtgatcg
     2641 ccgattcgct ggtcgccggg ttgatcgcgg gcgccatcgc tggctatctg ccgttcttcg
     2701 cgatgaactc caagcgcagc aagcgccagc ggcagctggc ggatcaaatc cccgatgcac
     2761 tcgatttcct ctcgcgcatt cttcgcgccg gtcacagctt gacgaccggc ctgcagatga
     2821 tgggcgaaga acttcgccaa ccgatggccg cggaattccg ccgctgctac gaccaacaca
     2881 gcctcgggca accgctcgag gattgtttga aggacatggc gacgcgcgtc gagaccaccg
     2941 acttcgcgtt cttcgtcacc gcggtgttga tccaacgtca aaccggcggc gatctctcgg
     3001 aagtgctcaa caacatcagc ggcatgatcc gccagcgcat ccgcctgcag cagcacgtga
     3061 aagccaagac cgccgaaggc cgcttcaccg gatacatcct cgtcgcgttc ccagcgatca
     3121 tgttcctcat cgcatggacg atgaaccccg gctacgccgg agtgctgctg cacacgagca
     3181 aaggcatgat gctgctctgc accgcgttcg gtttgcagat gctgggactg tgggcgatcc
     3241 gaaagctgac gacggtgaag gtttgaagtt tatcctgagc gcagcacgta ctcgtgcgca
     3301 gtcgaaggat ctagccgaga agtatgtgaa ggatcgaacc gcagaacatt gcagcagtcg
     3361 cttgcgtcat cgcgagcaag ccgagccgga cccgcgatga cgcaagcgac tgctacgcga
     3421 tgtgcgcaaa gggtcgttcg ccaacttctc ggctagatcc ttcgacttcg gcgagtacgc
     3481 ctccgctcag gacaagggac aagacatgga caacgaagca ctcatcttcc tctgcgtcgc
     3541 cggcgcgatc acgctcgtcg ggttttcgct gtacctgctg ttcgcccccg cgtcgaacga
     3601 gcgactgcgc agccgcctga acggcgacgc tgagcaggat gccaccgaag cgcccggcgc
     3661 cgcgggcgtc gtgccgctgt tgcagaaggt gggccaggcg gccgctcagc cgttcatgcc
     3721 caagacgcgc gaagcacagt ccgacctgcg ccgccggctg gggtatgccg gttactattc
     3781 caactcggcc atcaagatgg tcacgggcgc gaaggtcatc tgcctggccg gcgggttggg
     3841 cctgggctat ctcgccagca cgttcaccga caccttcatg atgcagatgg ccgcgctgtc
     3901 gatcggcggg ttgttcggtt atctcgcgcc gaccttctgg ttgaagtttt cgattaaggc
     3961 caactgcaaa gccctgacct acggcctggc cgatgcgctg gacttgatgg tggtctgcgt
     4021 ggaagcgggc ctcacgatcg attcggccat gcagcgcgtc gggcaggaga tggcgctggc
     4081 gcatcccgct atcagccgcg agctgggcat ctgccacatg gaaacccgaa tgggcctgtc
     4141 gcgcatggat gcgctgaaga acctgggcgt gcggaccaac tgcgaatcgc tgcagtcgct
     4201 gtcgtcgatg ctgatccagg cggatcgatt cggcaccagc atcgccgcgg cgttgcgcat
     4261 tcacgcggac acgatccgca tcaaccgcca tcacgcggcc gaagaattgg ccgccaaggc
     4321 atcggtcaag atgagctttc cgctggtgct gttcatcttc ccagccacat tcatcgtgct
     4381 ggccgggccc acggtgatcc agctgatgga cagcccgctg atgaaataac ggtgtaaagc
     4441 cggcgatggt atcgccggtc ctcataaacc acataaaccg gctcgacgaa tcaaccgaag
     4501 aggattaaga catggcaaac cttcacaccc acccgacggc aaagctgacc cagttggcgg
     4561 cgctggcaat cacgacgctg ctgcttgtcg ctgccggctg ccactccaat ccaaaccaga
     4621 agatcgcgca cggcgaagag ttcagcgatg aaaaggcggc gacatcggtc ggccagatgg
     4681 cgcaagcgca aaccgccgcc ggcgccaaga atgatggcat gctctacgat cacaatttcc
     4741 acggcaccaa gctgaattcg ctcggccaga ccaagctgga cctgatcgtg aaggggaccc
     4801 ccgcgggcga tccggtggtc gtttacctca acgtcccgca ggatggcttg gcggcccgcc
     4861 ggccagccgt gttggcctat ctccacgacg gcggcctgaa ggacgcgcag atcaagctga
     4921 tcgacggtcc cgccactggt gagaatactc cgtcggccaa gacgatgtcg aagatctaca
     4981 tggccaagga cggcagctac accggcgaga tggcggagga ccaggccgcc ggcagcgccg
     5041 gtggcatggg ccaaccgtcg tcgggcggca gccccagcgc ctcgcactga tcgctgctga
     5101 gcagcgccag aacaatcaaa tctgtgtctt ccggacggga aaatccggcg gcggtgagat
     5161 ccacgcgatc tcaccgccgc atgcgttttg cggaacaccg cagaggatca gctcgcgcct
     5221 cacgccgggt tcgcaggtac accgacgacc ggggttcttt ggcgcgccag aagaatccgg
     5281 gtcgtcgcga gtacgctccg aacccggcgt cgggagtccg ggcgagcatt cgcgcgccgc
     5341 taagctcaag cacttcgaat ccgcgcgcct ttggggcgcg taactgatgt tgtaggcact
     5401 cacgcagctc tttccgccca gaagtcggac cataagccgc tgtagtccgt tccttttccc
     5461 tcaccgcctg ttccagcacc gccagaccct tgccgtgcgg agcctccttc atcacctgcc
     5521 gtagctcccg ataatttaac aatgcctgct cagccaccag ccgctcttcc tcgttccgga
     5581 atctcatgcg atcctccgtg aaaa
//
LOCUS       DATIMH010000393         8152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2112265, whole genome shotgun sequence.
ACCESSION   DATIMH010000393 DATIMH010000000
VERSION     DATIMH010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112265"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VLI90_06790"
     CDS             complement(<1..968)
                     /locus_tag="VLI90_06790"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HSV13949.1"
                     /translation="MRLKLDKISWNTRSSIRRQLIWTLVPGVVALIVAAGAGLYVYVE
                     EILEHAVDTAVAAKAEALASMVRLEDDRQPHLRIPDQYATEFSSKDGPLFQIWRQDGS
                     TLARSTSLQNANVPQAKSFARGHRFGDVTMPDGSLGRATRMMFTPAPDEDVDENGKSV
                     ANRSNEQMRLVVAQGQRTTNQTLGVLLSGLLITAVLMTGGMLALVRWTVRRGLRPLDQ
                     IAADADRVGPEALDIRFPLESIPEELAPISRKLNELLDRLRAAFHRERRFSADVAHEL
                     RTPIAELRSLCEVSLRWPGDAQAATESFNEALSIARQMEATVATLLA"
     gene            complement(958..1644)
                     /locus_tag="VLI90_06795"
     CDS             complement(958..1644)
                     /locus_tag="VLI90_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSV13950.1"
                     /translation="MRLLLVEDSERLQRSVGTGLRKSGYAVDIVGTGPEGLWHAESHD
                     YDVIILDIMLPGFDGLTLLRRLREQGRETHVLLLTAKDTVADRVIGLRAGADDYLVKP
                     FAFDELLARIEALTRRKHGRKNPLIHIRDLQINTAARTVARGGKTIELFPREYALLEY
                     LAAHQGEVVSRTDIEAHIYDDRVEPLSNVVDSAVCALRRKIDPPHGPSLIVTRRRMGY
                     VLNPSSDDAS"
     gene            complement(2028..4175)
                     /locus_tag="VLI90_06800"
     CDS             complement(2028..4175)
                     /locus_tag="VLI90_06800"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSV13951.1"
                     /translation="MKKDQLPFLFGSQYYRAPTPEKECWAEDLAKMKDIGFNQVKYWV
                     QWRWQHRAPDRFYWDDLDRLMDLAGNNGIGVTLNAILDVAPIWLYERYPDAKQIDIKG
                     LVVEPFAVQHRQVGGQPGPCYNHAGALDERKKFLHAAVDHFRGHQAMQMWDVWNEPEQ
                     SYPRRHPDMNQLTCYCPSCRQKFSGWLRKKYGSLERLNDVWARCYEAWEQVEMPRTDA
                     CVTDFIDWREFHLDTMTSEAKWRLDMARKHDPASVSYLHVVPASMTIFNPVTCVDDFD
                     IAEHSDVWAATMNQSPTFTTQVVSAARGKVAYNVESHVNYGSLRRHQRILRLPDLLAD
                     WLPQIGLGVKGFMYWQFRPEVAGTESPAWGVVNLDGSDRPATEATRTFWKTIEPHVQA
                     IMNCPAPQGEVGIFKSRKNELYHFCMDKDLAPLASGVESYLHNLYWMNYRFRFVSGQM
                     LERGDMPGIKLLILPSAIYLSQPEVDALNTWVRNGGVVLSEAHLASYNATIGRHARQM
                     PGCGLSREWGIRETESTATQHLKLEQAGGLDGFMTADERKAWGEGVGGEYVPIRLTNG
                     ATAWGGGSRYALLESEDAETIGSFDGEHPTILLKRIGQGAVIYCGSNLGQGAKRDAAG
                     LREVLKLAATRAGVTATLDANADTPDVHVDLLANNGSPKFVLMWNRSSSERTVNLMLN
                     ARLRGLFTGPSHHCTGQTSITLRAHSVDLFQVE"
     gene            4441..6900
                     /locus_tag="VLI90_06805"
     CDS             4441..6900
                     /locus_tag="VLI90_06805"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HSV13952.1"
                     /translation="MKIKVLAGLAAVAAAVFVASPTFGQAKPRPIPTGPASVLQSLPN
                     ELFSGWKLTPAGRHVGVTSMPLKMVVSPDGKTLAAVCAGRWNGLALIDLATETTRQWV
                     PLYRTFNGVAFSRDGKQIFVTGGNSDRLYVFDFDGQKVRDEPTTIHLGNQPKGSQRDN
                     FFAGMTIDPDTGKLYLCNEGTSEIWVFDPATSKIEAKWRTEAHPYACAFGTEKKFLFV
                     SNWGDGSVSALDPQSGTQVARIGVGMRPNEMAVAPDGRLFVCCAGDNTVRVLQTQSPH
                     DDDRANKTNQKGPPPTDALEIISTSLYPSSPEGSTPNAVAVSADGKTLFVANADNNDV
                     MVADIADAKSSRVVGFVPTGWYPCAVATDGNKLFVANGKGLESSPSFPSKRPENNVMG
                     GVPFDPPLHILSGSVSIIDPPNGDQLLAYTKQVRDNCPYVPQDLKLASEPNQSVIPSK
                     VGDQGACPIKHVLYIIKENRTYDQMFGDMTDNAGKRIGNGNADICMFGDSVSPNQHQL
                     ARDYVLMDNLYVMSEVSWDGHAWCDSAISTDYGQRVWITNYTTHGHLPGNGETNATLA
                     GNIWDACKRAGVSYKCYGEGAWDAEKENRGTWPGGRDPQKAQGWIKDLHDAERTGDLP
                     SFMIMSLGENHTAGTTPGAFTPQAMVASNDQAVGQIVDAASHSKFWNQMAIFIVEDDA
                     QNGPDHVDAHRTDGLVISPYVKRNTIDSTHYTQMSMLRTMELILGLHPLTQFDAAATP
                     MFNLFTTQCVSTPFTNIAPKTDLLAKNAKTAPGAEASAKMNFEDYDDAPEDELNRILW
                     AAVKGEDTPYPTPIHRALFAN"
     gene            7514..8068
                     /gene="efp"
                     /locus_tag="VLI90_06810"
     CDS             7514..8068
                     /gene="efp"
                     /locus_tag="VLI90_06810"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HSV13953.1"
                     /translation="MRASEIRKGTAVIIDGKHYVVTGADHNTPGNLRAKVQFKLRDVG
                     KGTIMDKRVGATDDIEITTLDSRNVEYLYSDNDGHVLMDLETYDQNTIPKEVFGDDIL
                     YLKPNTQLTAMFHDGKIVSYELPKVVDLEITDTPPVIKGATATNQLKDATLETGLKTR
                     VPGFIVTGEKIRISTETGQYMSRS"
BASE COUNT         1589 a         2522 c         2534 g         1507 t
ORIGIN      
        1 gccagcagcg tcgcgaccgt cgcttccatc tgccgggcga tcgacagcgc ttcattaaac
       61 gattccgtcg ccgcctgcgc atcgcccggc caccggaggg agacttcgca aagcgagcgc
      121 agttccgcga tcggtgtgcg cagctcatgg gcgacgtcgg cgctaaatcg ccgctcgcga
      181 tgaaacgcgg cccgcaggcg gtcgagcagc tcgttcagct ttcggctgat cggcgcgagt
      241 tcctcgggga tcgactccag cgggaagcgg atgtccagcg cctcgggccc cacgcgatcg
      301 gcgtcggcgg cgatctggtc cagcgggcgc aatccgcggc ggacggtcca cctcaccagc
      361 gcgagcatcc cgccggtcat cagcaccgcg gtgatcagca ggccgctgag cagcacgccg
      421 agcgtttgat tggttgtccg ctgaccctgc gcaacgacca gtcgcatctg ctcgttgctg
      481 cggtttgcaa cggactttcc gttctcgtcc acgtcttcat cgggcgcggg cgtgaacatc
      541 atccgggtgg cacgaccgag cgagccatcc ggcatcgtca cgtcaccaaa gcggtggccg
      601 cgcgcgaacg acttggcttg cggaacgttg gcattctgta gcgatgtcga tcgcgccagc
      661 gtcgaaccgt cctgccgcca gatttgaaac agcggtccgt ctttgctgga gaactcggtg
      721 gcgtattgat ccggaatgcg caggtgcggc tggcggtcat cttccaatcg caccatcgag
      781 gccagcgctt ctgccttggc ggcgacggcg gtatccacgg catgttcgag gatttcttcg
      841 acgtaaacgt agagccccgc tcccgccgcg acaatcagcg cgacgacgcc gggcaccagc
      901 gtccagatga gctgtcgccg gatggaactc cgcgtgttcc acgagatttt gtcgagctta
      961 agacgcatcg tcactcgacg gattcagcac gtatcccatc cgccgccgcg tcacgatgag
     1021 ggacggcccg tgcggtggat cgatctttcg ccgcagcgcg cacaccgcgg aatcgacgac
     1081 gttgctgagc ggctcgacac ggtcgtcgta aatatgcgct tcgatgtcgg tgcggctgac
     1141 gacttcgccc tgatgggcgg caagatattc gagcagcgcg tactcgcgcg ggaaaagctc
     1201 aatggtcttt ccaccacgcg cgacggtgcg ggcggcggtg ttgatttgca gatcgcgaat
     1261 gtgaatgagc gggttcttcc gcccgtgctt gcgacgcgtc agcgcttcga tgcgcgccag
     1321 cagctcgtcg aacgcaaagg gtttgacgag gtaatcgtcg gcgccggcgc ggaggcctat
     1381 cacacgatcg gccaccgtgt ctttcgcggt cagcagcagc acgtgggttt ccctgccctg
     1441 ctcgcgcagg cggcgaagca gggtcagccc gtcgaagccg ggcagcatga tgtcgaggat
     1501 gatcacgtcg tagtcgtgcg actcggcgtg ccacaacccc tccggcccgg tgccgacgat
     1561 atcgacggcg taaccgctct tgcgcaggcc ggtgccgacg gatcgctgca ggcgttcgga
     1621 atcttcgacg aggaggagac gcatattcgc tttcactaga acgttagcgc gcccccattc
     1681 ccccctccgg cgtattcgcg cgcgagggtc ggggtgaggt gaattcgagc acgaatggta
     1741 cgaaattctt agatcaccct cacccttttc tatgaacaat cgctgctgtc aatagcgttg
     1801 gcactgctgg ggcactgcgt cacgaagcaa gactgggctt ttagcgagcc agcgccgatt
     1861 agccaccgcg gagtcgcggc gattttctgg cgcaacgcta cgttcgcgcc gcgagaccgc
     1921 ggcggctaaa cgatgcgcac tcttgttgtt gcggatggtc tttgagttgc gcccatttcc
     1981 ctgcgaaaac atcaagctga tcgcaactgg atcgacgcgt gcgcgaatca ttccacttgg
     2041 aacagatcaa ccgaatgggc ccgcagggtt atcgaggttt ggccggtgca gtggtgggac
     2101 gggccggtga acaagccgcg gaggcgggcg tttagcatca ggttcaccgt gcgctcgctg
     2161 gatgagcggt tccacatcaa cacgaacttc ggcgaaccgt tgttggcgag caggtcaacg
     2221 tggacatccg gcgtgtcggc gttggcgtcg agcgttgccg tgacaccggc gcgcgtcgcg
     2281 gcgagtttga ggacctcgcg taaaccggcg gcgtcgcgct tggcgccttg gccgaggttc
     2341 gagccgcagt agatcacggc gccttggccg atgcgtttga gcaggatcgt gggatgctcg
     2401 ccgtcgaagg agccgattgt ttcggcatct tcggattcga gcaacgcgta gcggctgccg
     2461 ccaccccagg cggtggcgcc gttggtcagg cggatgggga cgtattcgcc gcccacgcct
     2521 tcgccccagg cttttcgttc gtcggcggtc atgaaaccat cgaggccgcc ggcttgctcg
     2581 agcttgagat gttgggtggc ggtggattcg gtttcccgga tgccccattc gcgcgacagg
     2641 ccacaacccg gcatctgccg cgcgtgccgg ccgatcgtgg cgttgtagct ggccaggtgc
     2701 gcttcgctca gcacgacgcc gccgttgcgc acccaagtgt tgagcgcgtc aacttccggt
     2761 tggctcaggt agatggcgga tggcaggatg agcagcttga tgccgggcat gtcgccgcgc
     2821 tcgagcattt gcccggagac gaagcggaag cggtagttca tccagtacag gttgtgcaaa
     2881 tagctttcga cgccgctggc cagcggcgcg agatccttgt ccatgcagaa gtgatacagc
     2941 tcgttctttc ggctcttgaa gatgccgacc tcgccctgcg gcgccggaca attcatgatc
     3001 gcctgcacgt gcggttcgat tgtcttccag aacgtgcgcg tcgcttcggt ggcgggacga
     3061 tcgctgccgt cgaggttgac cacgccccac gcgggtgatt ccgttccggc aacttccggg
     3121 cggaattgcc agtacataaa acctttgacg ccaaggccaa tctgcggcaa ccaatcggcc
     3181 agcaagtctg gcaagcgcag aatgcgctgg tgccggcgaa ggctgccgta gttcacgtgg
     3241 ctttcgacgt tgtacgcgac cttgccgcgc gcggccgaca ccacctgcgt ggtgaacgtc
     3301 gggctctggt tcatcgtggc ggcccagacg tcgctgtgtt cggcgatatc gaaatcgtcg
     3361 acgcacgtga cgggattgaa gatcgtcatc gacgcgggga cgacgtgcag atacgacacc
     3421 gacgccgggt cgtgcttgcg ggccatgtcc aagcgccact tggcttcgct ggtcatggtg
     3481 tcgagatgga attcgcgcca gtcgatgaag tcggtcacgc acgcatcggt gcggggcatc
     3541 tcgacctgct cccacgcttc gtaacagcgc gcccacacat cgttgagccg ttcgagtgag
     3601 ccgtacttct tccgcaacca gccgctgaac ttttgccggc atgatgggca gtagcaggtg
     3661 agctggttca tgtccggatg acgccgtggg tacgactgct ccggttcgtt ccacacgtcc
     3721 cacatctgca tcgcctgatg gccgcggaag tgatcgacgg cggcgtgcag gaatttcttg
     3781 cgctcgtcga gcgcgccggc gtggttgtaa cacggcccgg gttgaccgcc gacctggcga
     3841 tgctgcaccg cgaacggctc gacgacgagc cctttgatgt cgatctgctt ggcgtcgggg
     3901 tagcgctcgt agagccagat cggcgcgacg tccaggatgg cgttgagcgt gacaccgatg
     3961 ccgttgttgc cggccaggtc catcaggcga tcgaggtcgt cccagtagaa gcgatcgggc
     4021 gcgcgatgct gccagcgcca ttgcacccag tatttaacct ggttgaagcc gatgtccttc
     4081 atcttggcca ggtcttcggc ccagcattct ttttcggggg tcggcgcgcg gtaatactgg
     4141 gaaccgaaca ggaacggcag ttgatctttc ttcatggggt actcacagat ggattgaacg
     4201 acgacaacaa cgcgaacgta caccgtgcag cgcgggtacg gatgcacgga aatagatatc
     4261 ggtttagatt attttcggaa gcccttttct gccgtctgta taccatgggc gcacgcctaa
     4321 tgaaatatta ttcgtcaaat tgaaaggttt cattgaagga tttgcgatgc cgcgataagg
     4381 ttcatctttc gctgcgcgga tgcatcgtct ttattgccgg atcgattcga ggattgtcga
     4441 atgaaaatca aggttcttgc tggacttgcc gctgttgccg cggctgtttt tgtcgcttcg
     4501 ccgacattcg gacaagctaa accccggccg attccaacgg gcccggccag cgtgctgcaa
     4561 tcgttaccga acgagctgtt cagcggctgg aagctcaccc ccgcaggccg gcacgttggc
     4621 gtcacttcaa tgccgctgaa gatggtcgtt tcgcccgatg gcaagacact ggccgcggtg
     4681 tgcgcgggcc gatggaacgg gctggcgctg atcgacctgg cgaccgaaac cacccgccaa
     4741 tgggtgccgc tctatcgcac gttcaacggc gtcgcgtttt cgcgcgacgg caaacagatc
     4801 ttcgtcaccg gcggcaattc cgatcgtctc tacgtcttcg acttcgacgg acagaaggtg
     4861 cgcgacgagc cgacaacgat ccatctcggc aatcagccca agggctcgca gcgcgacaac
     4921 tttttcgccg ggatgacgat cgaccccgat acgggcaagc tctatctctg caacgaaggc
     4981 accagcgaaa tctgggtctt cgatcccgcg acgagcaaaa tcgaagcgaa gtggcgcacc
     5041 gaagcgcatc catacgcgtg cgcgttcggc acggaaaaga aattcctgtt cgtgagcaac
     5101 tggggcgacg gctcggtcag cgcgctcgat ccgcaaagcg gaacacaagt cgcgcgcatc
     5161 ggcgttggca tgcggccgaa cgaaatggcc gtcgcgcccg atggccgatt gttcgtctgc
     5221 tgcgccggcg acaacaccgt gcgcgtgctg caaacccaat cgccgcacga tgacgatcgc
     5281 gccaacaaga ccaatcagaa aggcccgccg ccgactgatg cgctggaaat catctcgacc
     5341 agcctgtatc ccagttcacc cgaaggctcg acgcccaacg cggtggccgt gtcggccgac
     5401 ggcaagacgc tgttcgtcgc caacgccgac aacaacgacg tgatggtcgc cgacatcgcc
     5461 gacgctaaat cttcgcgcgt cgtcggcttc gtgcccaccg gttggtatcc gtgcgcggtc
     5521 gccaccgatg gcaacaagct cttcgtcgcc aacggcaagg ggctggaatc atcgcccagc
     5581 ttcccgtcga agcggcccga gaacaacgtg atgggcggcg tgccgttcga tccgccgctg
     5641 cacatcctca gcggcagcgt gtcgatcatc gatcccccca acggcgatca gctgctggca
     5701 tacaccaagc aggtgcgcga caactgcccg tacgtcccgc aggatttgaa gctggccagc
     5761 gagccgaatc agtcggtaat accgagcaaa gtgggcgacc agggggcatg tcccatcaag
     5821 cacgttctct acatcatcaa ggagaaccgc acctacgacc agatgttcgg cgacatgacc
     5881 gacaacgccg gcaagcgcat cggcaacggc aacgccgaca tctgcatgtt cggcgattca
     5941 gtttcgccca atcaacatca gctggcgcgg gattacgtgc tgatggacaa cctgtacgtg
     6001 atgagcgaag tctcctggga cggccacgcc tggtgcgaca gcgccatctc caccgactac
     6061 ggccagcgcg tctggatcac caactacacc acgcacggcc atctgcccgg caacggtgaa
     6121 accaatgcca ccctcgccgg gaatatctgg gacgcctgca agcgcgccgg cgtcagctac
     6181 aagtgctacg gcgaaggcgc ctgggacgcc gagaaggaaa accgcggcac ctggcccggt
     6241 ggccgcgatc cgcaaaaagc gcaaggctgg atcaaagacc tgcacgatgc cgagcgcacc
     6301 ggcgatctgc cgtcgttcat gatcatgagc ctgggcgaaa atcacaccgc cggcaccacg
     6361 cccggcgcgt tcacgccgca ggcaatggtc gcgagcaacg atcaagccgt cggccagatc
     6421 gttgacgcgg cgagtcactc gaagttctgg aaccagatgg cgatcttcat cgtcgaagac
     6481 gatgcccaga acggcccgga ccacgtggac gcccaccgca ccgacgggct ggtcatctcc
     6541 ccctacgtca agcgcaacac gatcgactcc acccactaca cgcaaatgag catgctgaga
     6601 acgatggagc tgatcctggg cctgcacccg ctcacgcaat tcgacgccgc cgccacgccg
     6661 atgttcaacc tcttcacaac gcagtgcgtg agcacgccgt tcacgaacat cgcgcccaag
     6721 acagatctgc tggccaagaa cgccaagacc gcccccggcg ccgaggcgtc ggcgaagatg
     6781 aacttcgaag actacgacga tgcgccggag gatgagctga accgaatcct ctgggccgcc
     6841 gtgaagggcg aggacacgcc gtatccaacg ccgatccatc gcgcgctgtt tgccaattga
     6901 atgagtccga aacggcgtta ccccatgtta cgaaatgtta cgttttcatc tgaaaacgta
     6961 acattcttcg caaatttgct atagattggc gaagtgctcg gcgaaggcac gaggcgtggc
     7021 ttccgcggga atttgcgcac cgagataccg ccattgcgtc accgtttcac agcccgcctc
     7081 gtgcttccac tgctcacgga agatcaacag ctccctcgcc cggcggcgtc tgcaccagga
     7141 tcccgctcgc ccagtcgccc gcttcgcagt tgcacagcgt tgcgcacagc aaagtcgtca
     7201 acgggagaaa catgccgacc acgccgggtt cggagcagcc tgccctgagc ttgtcgaagg
     7261 gtacccgcga cgacccggat tcttccgcgc gcgagagcag aagcatcggg gtcgtcggag
     7321 tacctccgaa cccggcgtgg aggttaataa tcgtcgcccg cgcttttgac gcgcgctgtt
     7381 cggcccgcta catttgccga tctttcccgt tccttgcagc gtactgattg ttgggaaagt
     7441 cttgcgcatt ggtgcctgcc gagtgtgtgg gtacggagcg tttgtgtcac ttaagtttag
     7501 aaggtaagga aacatgcgag cgagcgaaat tcgcaaggga actgccgtca tcatcgacgg
     7561 caagcattac gtcgtcaccg gggccgacca taacacgccg ggcaacctgc gggccaaggt
     7621 gcagttcaag ctgcgcgacg tgggcaaggg cacgatcatg gacaagcgcg tcggcgcgac
     7681 cgacgacatc gagatcacga cgctggacag ccggaacgtc gagtacttgt acagcgacaa
     7741 cgacgggcac gtgctgatgg acctggagac gtatgaccag aacacgatcc ccaaggaagt
     7801 gttcggcgac gacatcctgt acctcaagcc caacacgcag ctgacggcga tgttccacga
     7861 cgggaagatc gtcagctacg agctgccgaa ggtggtggac ctggagatca cggacacgcc
     7921 gccggtgatc aagggcgcga ccgcgacgaa ccagctgaag gacgcgacgc tggagaccgg
     7981 gctgaagacg cgcgtgccgg gatttatcgt gacgggcgag aagatccgga tcagcacgga
     8041 aaccgggcag tacatgagcc ggtcgtgatc atcgtagctg cgacgcctgc gtcgcctctt
     8101 cgggcaaagg cgacgcgggc gtcgcagcta cagaaacaaa agcaattact ac
//
LOCUS       DATIMH010000394         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3269128, whole genome shotgun sequence.
ACCESSION   DATIMH010000394 DATIMH010000000
VERSION     DATIMH010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3269128"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VLI90_06815"
     CDS             complement(<1..1209)
                     /locus_tag="VLI90_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSV13954.1"
                     /translation="MSTDAQPTAEKKSYKQTLNLPQTAFAMEAKLVQNEPKRLAEWDE
                     ARLYEKIITARANSPKGRWVLHDGPPFANGDIHIGHVINKTLKDVILRFRTMEGYQTP
                     YVPGWDCHGLPIEHKIQEQIKKEGKRIHELGTLEVRRRCFEYANKYVQVQCEQFKRLG
                     ILGEWNHPYLTMKPRYEASTLEVFAKFVEAGLVYRKLKPVPWSIANQTALADAELEYQ
                     DVQDQSVYVEFPVANEEVARKLFPLGVQYPFALLVWTTTPWTLPANLAVAVHPDMSYV
                     AVAYELNGRSRVGIVAEELAGTVMGKPAKDDFKAYMEALSPPVKGREIVEAGLTYRHP
                     FIERTGRVVLADYVTTTDGTGLVHTAPGHGEDDYETGIREGLDVYSPVLANGRFDDTV
                     PEWLRGKSAQK"
     gene            complement(1288..2742)
                     /locus_tag="VLI90_06820"
     CDS             complement(1288..2742)
                     /locus_tag="VLI90_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HSV13955.1"
                     /translation="MSDAAPSQILDYPSGSSDHPRPLRELLFLAAPTVAQMASYTLMQ
                     FIDTWMLAHATRGSGVAAPTAAVNAGILAFACISLGMGVMFVVNTLVSQAFGRRDFPA
                     CGQFMWQGVWAGLLFSAALSPLAVFAPRVFRFFGHEPIIAGMEGTYLRIVVGAAVLKL
                     TTTALEQFFLGVNRPGAVGVATVGGVAVNAAAAWVLIFGHFGLQPMGVAGSAIAQNIG
                     VGAEMCAMIAFATGPVVRRQFLLHDWRFRSGEMWTLLKIGIPAGLQVVADVLAWSAFS
                     IWVMGRFGNNTMAANVFVFRYMSVSFMPAFGISVAVTALVGRYIGMGRPDIAVRRAHL
                     GFAVTALYMVACGVMLFCFRRQLIGLFTDDPVVTQVGATLMVFAAVWQLFDAMYIVYN
                     GALRGAGDTFVPAVVLAVLCWSIAVGAAYVVATHRPELGAVGPWTCAVIYGVLLGIFM
                     LTRFSRGGWKRIQLKTDSQASNLQLDSSTFAAAK"
     gene            complement(3041..>3264)
                     /locus_tag="VLI90_06825"
     CDS             complement(3041..>3264)
                     /locus_tag="VLI90_06825"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HSV13956.1"
                     /translation="PLPGSPAIDAGDNTLVPAGITTDTRGFARITHGKVDMGAVETRF
                     SGVFAEPALPAGLPPTNLVINTLTSLGLL"
BASE COUNT          638 a         1118 c          923 g          585 t
ORIGIN      
        1 cttctgcgcg ctcttgccgc gcaaccactc cggcacagtg tcatcgaatc ggccgttggc
       61 aagaaccggg ctgtagacgt ccagcccttc gcgaatgccg gtttcgtagt cgtcttcacc
      121 gtgcccgggc gctgtgtgca ccaagccggt tccgtccgtg gtcgtcacgt agtcggccag
      181 caccactcta ccagtgcgct cgatgaaggg atgacggtag gttagtccag cctcgacgat
      241 ctctcttccc tttaccggtg gcgacagtgc ttccatgtat gctttgaagt catccttcgc
      301 cggcttaccc attaccgtac ctgcgagctc ttccgcaacg atgccgacgc gcgagcggcc
      361 attcagttca taagctaccg ccacgtacga catgtcagga tgcacagcca ctgcaaggtt
      421 ggccggcaac gtccatggcg tcgtcgtcca gaccagcagc gcgaatggat attgcacacc
      481 aagagggaat agctttcgag ccacctcttc gttagctacc ggaaattcca cgtacacgct
      541 ctgatcctgc acgtcctgat attccagctc cgcatccgcc agcgccgtct gattcgcgat
      601 cgaccacggc accggtttga gcttgcgata caccagcccc gcctcgacga atttcgcgaa
      661 cacctccagc gtgctcgctt cataccgcgg cttcatcgtc aggtacgggt ggttccactc
      721 gccgaggatg cccagacgtt tgaactgctc gcattgcacc tgcacatatt tgttggcgta
      781 ctcgaagcat cgccggcgca cttccagcgt gcccagctcg tggatgcgct tgccttcctt
      841 cttgatctgt tcctggatct tgtgctcgat cggcagcccg tggcagtccc accccggcac
      901 gtacggcgtc tggtaaccct ccatcgtgcg gaagcgcagg atcacatcct tcagcgtctt
      961 gttgatcacg tgcccgatgt ggatgtcgcc attggcgaac ggcggaccat cgtgcagcac
     1021 ccagcggccc ttggggctgt tggcgcgtgc cgtgatgatc ttttcgtaca gccgggcctc
     1081 gtcccattcc gccaggcgct tgggctcgtt ctgcacgagc ttggcttcca tcgcaaacgc
     1141 cgtctgcggg aggttcagcg tctgcttgta gctcttcttc tcagccgttg gttgtgcgtc
     1201 agtcgacatt ttgtagcacg ggctacgagc tttggctctc tcgcccgcgt ccaagcatcc
     1261 aggcacgggc gggaggcccg tgctacttta tttcgcagcg gcaaatgtag acgaatccaa
     1321 ctgcaagttc gaggcttgcg aatccgtctt cagctgaatc cgtttccatc caccgcggct
     1381 gaaccgcgtg agcatgaaga tgcccagcaa tacgccgtag atgacggcac aggtccatgg
     1441 accgaccgcg cccaactccg gccggtgggt cgcgacgacg tacgccgcgc cgacggcgat
     1501 gctccagcac aacactgcca gcacgacggc cggcacaaac gtgtcgccgg cgccgcgcag
     1561 cgcgccgttg taaacgatgt acatcgcgtc gaacaactgc cagacggcgg cgaaaaccat
     1621 cagcgtggcg ccgacctggg tcacgaccgg atcgtcggtg aacaggccga tcaattgccg
     1681 gcggaagcag aacagcatca cgccgcacgc gaccatgtac aacgccgtga cggcaaaccc
     1741 caggtgcgcc cggcgcaccg cgatgtccgg ccggcccatg ccgatgtatc gccccaccag
     1801 cgcggtgacg gccacgctga tgccgaatgc cggcatgaag ctcaccgaca tgtagcgaaa
     1861 gacgaacacg ttggcggcca tcgtgttgtt gccgaatcgg cccatgaccc agatcgaaaa
     1921 cgccgaccac gccaacacgt ccgccacgac ctgtaatccc gccgggatgc cgatcttcag
     1981 cagcgtccac atctcgcccg atcgaaatcg ccaatcgtgc agcaggaact gccggcgaac
     2041 caccgggccc gtggcgaacg cgatcatcgc gcacatctca gcgccgacgc cgatgttctg
     2101 cgcgatggcc gagccagcga cgcccatcgg ctgcaacccg aaatgtccga agatcaacac
     2161 ccacgccgcc gccgcgttga ccgcgacgcc gccgaccgtc gccacgccaa cagcacccgg
     2221 ccgattcacg ccaaggaaga actgctccag cgccgtcgtc gtcagcttca acaccgccgc
     2281 cccgacgacg atgcgcagat aagttccttc catccccgcg atgatcggct cgtgtccgaa
     2341 gaaccgaaac acgcgcggcg cgaacaccgc caacggcgac aacgccgccg agaacagcag
     2401 gccagcccac accccctgcc acatgaattg cccgcacgcc gggaaatcac gccggccaaa
     2461 cgcctgactc accagcgtgt tcacgacgaa catcacgccc atccccaggc tgatgcatgc
     2521 gaatgctaaa atccccgcat tcaccgccgc cgtcggcgcc gccaccccgc tgccgcgcgt
     2581 cgcgtgcgcg agcatccacg tgtcgatgaa ctgcatcaac gtataagagg ccatctgcgc
     2641 cacggtcggc gcagccagaa acaacagctc gcgcagcggg cgcgggtgat cggacgatcc
     2701 agacgggtaa tcgaggattt gcgatggagc ggcgtcgctc atgatctgtt tccgccaaat
     2761 aaaaaacccc gaggccgcgc gtcgcgcctc agggttgatt gtttgcgtga aagacgcgcc
     2821 aagccctagc cgcgacgaaa acgtcgaatg cgaataatcg ccggaatagc tggaatgggc
     2881 gtcatctatc aatcctcggt caggatcgta tcggcgatat gggggaagtc aacgtagcgt
     2941 ttcccgcccg cgcagcataa caagtgcatc acgccgcttg cggcttcaca atctcgacaa
     3001 cgttcgtctg cgaaaccgca agcggcggtc agcgatatct ctacagcagc cccaggctcg
     3061 tcagcgtatt gatcaccagg ttcgtcggcg gcaggcctgc cgggagcgcc ggctccgcga
     3121 agacgccgct gaagcgcgtc tccaccgcac ccatgtccac cttgccatgc gtgatgcggg
     3181 cgaacccgcg agtgtcggtg gtgatgccgg ctggcaccag tgtgttgtca ccggcgtcga
     3241 ttgccgggct gcccggcagc ggtg
//
LOCUS       DATIMH010000395         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2980586, whole genome shotgun sequence.
ACCESSION   DATIMH010000395 DATIMH010000000
VERSION     DATIMH010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2980586"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VLI90_06830"
     CDS             <1..876
                     /locus_tag="VLI90_06830"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13957.1"
                     /translation="GSPIRDRALLIQTLLAVQPENAALPDLVQRLADSGVRREWASTH
                     DTALAVLAIGRYLRESKRHEPYDTAQLALGEQVLASASEGGSIGWNAPPGFDASSAPL
                     LVKIAGADNATAHVSWLKTGVPLTPPADAEHGMKVRRRYLAINGDELRGSVRSGDLVR
                     VELTLQAPPQQAGIVIEDMLPAGLEVENPRLETSAKDRAKDDRPTDVPGFDDNRLDVM
                     DDRVVIVGSMPSAWKAKCTYLARAVTPGVYVVPPVRAEQMYDINVNALSGAGATLTVT
                     SATSNIASVHDDAAP"
     gene            1072..>2499
                     /locus_tag="VLI90_06835"
     CDS             1072..>2499
                     /locus_tag="VLI90_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSV13958.1"
                     /translation="MPVLFALAFGAAVAWWPYPRGVDRAPPSCTWIEDRGGTPLAAFA
                     SSSGDWHLPLSESEMSPHLLDAIVAVEDARFYEHGGVDWRSAIGAMWQDLTTLSLHRG
                     ASTITMQLHRLRDPAPRSLAAKVSQAVRACQIERFATKRQILVEYLNRAPFGGNLVGA
                     GAASWRYFGRPCRELSLGQAALLAGLPQGPNRFRPDRFPARARQRRDHVLDRMLACGM
                     ITAAERREAANEPTDARWGELPQYLADPAKSADGLLPALTRLAARHPGETIHTTFDAA
                     TQRQTAAAARAQLAALAASHISAVAVVVLDTSSGECLASVSCGDESAKAIDLTARPRS
                     SGSTLKPFIYAAAFDAGVCTPQTVLDDSPSSWSGYEPANYDRAFRGPLVAAEALAQSR
                     NVPALALLSKVGVDRAVEVMRAMGLATLSRTPQRYGLPLAIGGAEVTPMELAQAYAAL
                     ARGAVLTGNSPGRKAGDQSANHAGFS"
BASE COUNT          386 a          757 c          958 g          398 t
ORIGIN      
        1 ggctcgccga tccgcgatcg cgcgctgctg atccagacgc tgctggcggt gcaaccggag
       61 aacgcggcgc tgccggatct ggtgcagcgg ctggcggaca gcggcgtgcg ccgcgagtgg
      121 gcgagcacgc acgacaccgc gctggcggtg ctggcgatcg gtcgatatct gcgagagtcg
      181 aagcggcatg agccgtacga caccgcccag ttggcgctgg gcgagcaggt gctggccagc
      241 gcgagcgagg gtgggtcgat cgggtggaat gcgccgccgg ggttcgatgc ctcgagcgcg
      301 ccactactcg tgaagatcgc cggcgcggac aatgcgaccg cgcacgtctc gtggctgaag
      361 accggcgtgc cgctgacgcc gccggcggat gccgaacatg ggatgaaggt tcgccgccgg
      421 tatctggcga taaatggtga cgagctgcgc ggctcggtgc gcagcggcga tctggtgcgc
      481 gtcgagttga cgctccaagc cccgccgcag caggcgggga ttgtcattga agacatgctg
      541 cccgcggggc tcgaagtgga gaacccgcgg ctggagactt cggcgaagga tcgcgccaag
      601 gacgatcggc ccaccgatgt gcccggcttc gacgacaatc ggctggacgt gatggacgat
      661 cgcgtggtga tcgtcggctc gatgccaagt gcgtggaaag cgaaatgcac ctacctggcg
      721 cgagcggtga cacccggggt gtatgtcgtt ccccccgtgc gggccgagca gatgtacgac
      781 atcaacgtca atgcactgag cggcgccggc gcaacgctga cggtgacgtc cgcgacgagc
      841 aacatcgcga gcgtgcatga tgacgcagcg ccgtagatcc aagagcgatc cttaatagcc
      901 gcaggcgaca gccagcgggg gcgcgagaag tcggcccggt tccgattcca gaacccccgt
      961 tggctgtcgc ctgcggctgt caaatgcatg gactgccaga ctccacagac atccccgcgc
     1021 aagggtaagc gatgcgctcg tcgacgtgcg ctgttcattg cagccctcgc gatgcccgtg
     1081 ctgttcgcgc tcgccttcgg cgccgccgtt gcgtggtggc cgtatccgcg cggggtcgat
     1141 cgcgcgccgc cgtcgtgcac ttggatcgaa gaccgcggcg gcacgccgct ggcggcgttt
     1201 gcttcttcgt ctggcgattg gcatctgccg ctgagcgaat ccgagatgag cccgcacctg
     1261 ctcgacgcga tcgttgcggt ggaagatgcg cggttctacg agcatggcgg cgtcgattgg
     1321 cgatccgcca tcggggcgat gtggcaggac ctgacgacgc tgtcgctgca ccgcggggcg
     1381 agcacgatca cgatgcagtt gcatcggctg cgcgatcctg cgccgcggtc gctggcggcg
     1441 aaagtctcgc aagccgtgcg ggcgtgtcag atcgagcggt tcgcaacgaa gcggcagatc
     1501 ctcgtcgagt atctcaatcg cgcgccgttc ggtgggaacc tcgtcggcgc gggggcggcg
     1561 agctggcgtt acttcgggcg gccttgccgg gagttgagcc tgggacaggc ggcgctgctc
     1621 gccgggttgc cgcagggccc gaaccggttt cggccggatc ggttcccggc tcgcgcgcgg
     1681 caacgccgcg atcacgtact cgaccgcatg ctggcgtgcg ggatgatcac ggcagccgag
     1741 cgccgcgagg cggcaaacga gccgaccgac gcgcgatggg gcgagttgcc gcaatacctg
     1801 gcggatccgg caaagtcggc ggatggtttg ctgccggcgc tgacgcggct ggcggctcgg
     1861 catccggggg agacgatcca cacgacgttc gacgcggcaa cgcagcggca aaccgcggcg
     1921 gcggcgcggg cgcagctggc ggcgctggcc gcttcacaca tcagcgcggt tgccgtggtc
     1981 gtgctggata catccagcgg agaatgcctc gcgtcggtga gttgtgggga cgagagcgcg
     2041 aaggcgatcg acctgacggc gcggccgcgg tctagcgggt cgacgctcaa gccgttcatt
     2101 tacgccgcgg cgtttgacgc gggggtgtgt acgccgcaga cggtgctgga tgattcgccg
     2161 agcagctggt cggggtatga gccggcgaat tacgaccgcg cgttccgcgg gccgctggtg
     2221 gcggcggagg cgttggcgca gagccggaac gtgccggcgc tggcgctgtt gtcgaaggtc
     2281 ggcgtcgatc gcgcggttga ggtgatgcgc gcgatggggc tggcgacgct gtcgcgaacg
     2341 ccgcagcggt atggattgcc attggcgatt ggcggggccg aggtgacgcc gatggagttg
     2401 gcgcaggcgt acgcagcgct ggcgcgcggg gcggtgttga ctggtaatag cccgggccgg
     2461 aaggccgggg atcaatcggc gaaccatgcg ggtttctcg
//
LOCUS       DATIMH010000396         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4423421, whole genome shotgun sequence.
ACCESSION   DATIMH010000396 DATIMH010000000
VERSION     DATIMH010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4423421"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VLI90_06840"
     CDS             complement(<1..430)
                     /locus_tag="VLI90_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13959.1"
                     /translation="MTREQLQEQCELGQRQLMATEYLDAIDTLAAAEAVAWANRDYDT
                     LARLYMPLQEARRQARQRCGEGTVRFDIIARAPGDAIDPEQLPNHGQLLVAGWASMEP
                     ALRVRAIARERQQYVETFLAAAFPVAGSADPAIVVVPLDDV"
     gene            590..1174
                     /locus_tag="VLI90_06845"
     CDS             590..1174
                     /locus_tag="VLI90_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13960.1"
                     /translation="MNSATPAVLFADQMKLAADSKATVMALRQVVPSLALDLKNARLD
                     VLQLAKSPANIALVNALHNHAYQFGAMLLVDYNKLIALSTRAMNKVMVDGFKLKLKPT
                     DTTLQAKLQADMVAFATAVVGPSAKFAADMTAAGTTLATDAAALAAANPTVTKLATDL
                     QQLQGDAGTAFSKVQIALAQGGADLNQFVTDLTT"
     gene            complement(1367..3208)
                     /locus_tag="VLI90_06850"
     CDS             complement(1367..3208)
                     /locus_tag="VLI90_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV13961.1"
                     /translation="MRDRAKDNAELLEEYRSRDSDQAFGALVERYIHIVYTAARRQVR
                     DAHLAEDITQAVFIVLSKKADSLRNPAALGGWLLQTTHHVACNALRMERRRTRHEQEA
                     AAMMPTIVEPGRESLSRRVSCVLDDALARLRERDRNAIVLRFLEGKSVIDVGSAMGIS
                     EAAAQKRIVRALTRLRDLFARRGIVTAADALPETISGQALLSAPPGLIVAVSAASTGI
                     PAGTGAALLAKATVKAVFWTNLKLAAIGTAATVAITIGVAVVASSAPTGPTGPTGPTG
                     ATPTAPAAAPISSAITSTQPVSNSTVQNFPNVLLVGPSTDTFLVGVDANTRRTPASDP
                     AAYIRSSAPATKQVSQGYGASMEGLRGKRVRLSAWIKTRNVTNWSDIEMSVLGYGQRV
                     LAECELYDHAITGTTDWQQCAVVADVPPDAQSIVFTSNLYGKGEMWTDGFRIETVGED
                     VPTTDDQKWHAWSPVLGQCSAALDPTTLRNGHPTMRVTCNAKSWVAYDHADRHVEQYL
                     GKRVRVSAWLKAERVSVVSGLWIRARGPNYKKIIDEGNWGTWATPGAKPLKGTVGWTQ
                     FVCELDVPLDTQYIVSGMIHNGTGTMWIDDFKFELVDAPAGGGGGEK"
     gene            complement(3703..>4217)
                     /locus_tag="VLI90_06855"
     CDS             complement(3703..>4217)
                     /locus_tag="VLI90_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13962.1"
                     /translation="IFDDQGIDVIAAGNYYGENGTLAGLPDADDMTNEELLACDCDVL
                     IPAATENTVDAAIAPHVKARIMVEAANGPTTPEADEILRDNGVTVLPDILANAGGVTV
                     SYFEWVQGLENFFWDKKRVEDELQRMMEKAFNAVNAKADEADCDYRTAAYTIAIARVA
                     ESCRLKGLFP"
BASE COUNT          759 a         1394 c         1271 g          793 t
ORIGIN      
        1 cgacatcgtc cagcggcacg accacaatgg cgggatcagc gctacccgca accggaaacg
       61 ccgcggcgag aaaagtttcg acgtactgct gacgctcgcg cgcgatcgcc cgcacgcgaa
      121 gggctggctc catggatgcc catccggcca cgagcagttg cccatgattg ggaagctgct
      181 ccggatcgat cgcatcaccc ggtgcgcggg cgatgatgtc aaatcgcacc gtcccctcgc
      241 cacatcgttg ccgggcctgt cgccgcgcct cctgcagcgg catatacagc cgggccagcg
      301 tgtcgtaatc gcgattcgcc cacgccaccg cttccgccgc cgccaaagtg tcaatcgcgt
      361 cgagatactc cgtcgccatc agctgccgct ggcccagctc gcattgctcc tgtaactgct
      421 cgcgcgtcat ggcgtcggat tgtaaacgaa gcggcggatt atcggacggg ctgcgtctca
      481 ggaaaaattc gaagtgccga tttgcccacc tcagaagtga atcgtacatc aatcccagaa
      541 ggaggattca attatgaatc gctatgtgga gactctcgag gaccggacgt tgaactctgc
      601 tacccccgcg gtgttgtttg cggaccagat gaagttggcc gcggactcca aggccacggt
      661 gatggccctg cggcaggtgg ttccctcgct cgcgcttgac ctgaagaacg ctcggcttga
      721 tgtgctgcag ctcgcgaaaa gcccggcgaa tattgcgctg gtgaacgcgc tgcataacca
      781 cgcatatcag tttggcgcga tgttgctggt ggactacaac aagctgatcg cgctttcgac
      841 ccgggcgatg aacaaggtga tggtcgatgg gtttaagctg aagctcaagc cgaccgacac
      901 gacgctgcag gcgaaactgc aagcggacat ggtggcgttt gccaccgccg tcgtcggccc
      961 cagcgcgaag ttcgccgccg acatgactgc cgccggcaca acgctggcca ccgacgcggc
     1021 cgctctggcg gccgccaacc cgaccgtcac caaactggcg acggacctgc aacaactgca
     1081 aggcgatgca ggcaccgcgt tcagcaaggt gcagatcgcg ctggcccagg gcggggcgga
     1141 tctgaatcag tttgtcacgg acttgacgac ctgatcgaac gcgcggttac agaaacggtt
     1201 gaatcaaacg acgggcccgc accaagtgcg ggcccgcctt cattcataca cgccgctgcg
     1261 cgcacccgat gattgagagt tcaaaccggc acgacttctc gacgtgaccc ggcatgtgtt
     1321 cacgccgggc taataaatgg ggtcttggga aaagatatga atcccgtcat ttttcgccgc
     1381 cgccgccgcc cgcgggcgca tctaccagtt cgaacttgaa atcatcgatc cacatcgtcc
     1441 ccgtgccgtt gtgaatcatc cctgaaacga tgtactgcgt gtccagcggc acatccagct
     1501 cgcatacgaa ttgcgtccat cccaccgtgc ccttcagcgg tttggcgccc ggcgtcgccc
     1561 acgtgcccca gttgccttca tcgatgatct tcttgtagtt cggccccctc gcgcggatcc
     1621 acagcccgga aaccacgctc acgcgctcag ccttcaacca cgccgacacg cgcacgcgct
     1681 tgcccaggta ctgctcgacg tgccggtcgg cgtgatcgta cgccacccag ctcttggcgt
     1741 tgcaggtgac ccgcatggtt gggtggccgt tgcgcagcgt cgtcggatcc agcgccgccg
     1801 agcactgccc aagcaccggg ctccaggcgt gccacttctg gtcatcggtc gtcggcacgt
     1861 cttcgccgac ggtttcgatg cggaaaccgt cagtccacat ctcccccttg ccgtaaagat
     1921 tagacgtgaa gacgatcgac tgcgcatcgg gcggcacgtc ggccacgacc gcacattgtt
     1981 gccaatccgt cgtgccggtg atggcgtggt cgtatagctc gcactcggcc agcacgcgct
     2041 gcccgtagcc caacacggac atttcgatat cgctccagtt ggtgacattt cgcgtcttga
     2101 tccacgctga cagacgaacg cgcttgccgc gcaagccctc catcgaggcg ccataaccct
     2161 gcgacacctg cttcgtggcc ggcgctgacg atcggatgta cgcggcggga tcgcttgcag
     2221 gcgttcggcg cgtattggca tcgactccga cgaggaaggt gtctgtgctc gggccaacaa
     2281 gcagcacgtt ggggaaattc tgcaccgtcg agttcgacac cggctgtgtc gatgtgatag
     2341 cggacgagat cggcgcggcg gcgggggcgg tgggtgtggc gccggttgga ccggttggac
     2401 cggttggacc ggttggcgcg ctggacgcca ccaccgccac gccaatcgtg atcgcgactg
     2461 ttgcggccgt cccgatcgcc gccagcttca aattcgtcca gaaaaccgcc ttcaccgttg
     2521 cctttgccag caacgccgcg ccggttccgg ctggaattcc ggtcgaggcg gcgctgaccg
     2581 ccacgatcaa ccccggcggc gccgacagca gcgcctggcc ggaaatcgtc tcaggcagcg
     2641 catccgccgc cgtgacgatt ccgcgccgcg cgaacaagtc gcgcaatcgc gtgagcgctc
     2701 gcacgatccg cttctgcgct gccgcttctg aaatgcccat cgcgctgccg acatcgatga
     2761 cgctctttcc ttcgagaaac cgcaggacga ttgcgttgcg gtctcgctcg cgcaagcgcg
     2821 ccagcgcgtc atccagcacg cagctcaccc ggcggctcaa cgattctcgc cccggttcaa
     2881 cgatcgtcgg catcatcgcg gccgcctcct gctcgtgccg cgtccgccgc cgttccatcc
     2941 gcagcgcgtt gcacgcgacg tgatgcgtcg tctgcagcag ccatccaccc agcgccgccg
     3001 ggttgcgcaa cgaatcggcc ttcttcgaca ggacaatgaa caccgcctgc gtgatgtcct
     3061 cggccaaatg cgcatcccgc acctgccgcc gggcggcggt gtacacgatg tgaatgtagc
     3121 gctcgaccag cgcgccaaac gcctgatcgc tgtcgcgcga acggtattcc tccagcagtt
     3181 ccgcgttgtc cttcgcccga tcgcgcatcg catcatcttg taaccggccg gccgctaaac
     3241 acgacacgaa tttcgggcag tttgggaatc tgatgtaggc gttttctagt caatgacttg
     3301 cgcccgacag cggcccgcac cagcggtgga gctgcggcaa cctctacgac aattgccaag
     3361 gcgttttcaa aacacaggac cgccaataca tgggctccgg ccacggctgg gcgaggcgaa
     3421 tagcgtttat cggaactgcc gaggatcgat tatggtggag tctccgccga cggcggagaa
     3481 tttcgtgatc tgcttagtgg gcgagcgcgt cgagcccagc ttcggcgaag ttggggcacc
     3541 ggcgcgggtg cgtcgaaggt ttcgcaacgc cggtggcgcc gccaagtctg tatcgcgccc
     3601 agttgtccga ccgatgagcg aggcgcggtc gcttcagcga ccatgccccc tttggaattt
     3661 gaacagacat ggccgctgaa gcgaccacgc cgcgccagga ttttagggaa acaatccctt
     3721 caaccggcac gactccgcga cgcgggcgat cgcgatcgtg tacgccgcgg tgcggtagtc
     3781 gcagtccgct tcgtccgcct tggcgttcac ggcgttgaag gctttttcca tcatgcgctg
     3841 cagctcgtct tcgacgcgct tcttgtccca gaaaaaattc tccaggccct gcacccattc
     3901 gaaataacta accgtaacgc cgccggcgtt ggcgaggatg tctggcaaca cagtgacgcc
     3961 gttgtcgcgc aggatctcat ccgcctcggg tgtggtgggg ccgttggcag cttcgaccat
     4021 gatgcgggcc ttcacgtgtg gcgcgattgc cgcgtccacc gtgttctccg tcgcggccgg
     4081 gatcagcacg tcgcaatcgc aggcgagtaa ctcttcgttg gtcatgtcgt cagcgtccgg
     4141 gaggccggcg agcgtgccgt tctcgccgta gtagttgccg gccgcgatca cgtcgatgcc
     4201 ctggtcgtcg aagatgc
//
LOCUS       DATIMH010000397         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_959611, whole genome shotgun sequence.
ACCESSION   DATIMH010000397 DATIMH010000000
VERSION     DATIMH010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959611"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..2664)
                     /locus_tag="VLI90_06860"
     CDS             complement(79..2664)
                     /locus_tag="VLI90_06860"
                     /inference="COORDINATES: protein motif:HMM:NF028477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HSV13963.1"
                     /translation="MNQSVMILVMTAAAALFAHGLPARAADTDLPPLRPPAVPLVTTD
                     PYFSIWSFDDHLNDADTHHWTGKPQTLLSLVRIDGKAYRLAGAQPSDAPAMQQTNLLV
                     LPTRTFYTFTGGGVTLTLTFTTPMLPVDLDVLSRPLTYVTWNAVASDGQSHQVSAMLA
                     MGGDIAVNTPDEAVVAHRQTFGTLTAMSIGSEAQPILRSKGDDHRIDWGYLYVTARDA
                     AGMAIGNAGVLASTFADSGNLPASDDTRFPRPVRDGLPSAAVVVDLGAVGNQNVARHA
                     MVAYDDLFSIQYMKRNLRPYWRRNGMDAGRLLQTAEAEYANLAERCAKFDDDLIGDAR
                     STGGEAYARICALAYRQALAAQKIVADANGMPLAFCKENFSNGCIATVDVIYPQLPQL
                     LLTSPALAKAALVPLLDYASSDRWHFDFAPHDLGTYPQANGQVYGGGERTEDNQMPVE
                     ESGNMLALLAAVAKADGNADFAGKYWPAITRWAKYLEAKGFDPEKQLCTDDFAGHLAH
                     NTNLSIKAIMGLGSYSMLCETRGDKTEAARVRAIARSFADRWVTEAADGDHSRLTFDK
                     RGTWSQKYNLVWDKVLGLNLFPKSVAQKEIAFYLTKQNKYGLPLDSRSTYTKLDWTIW
                     TATLADRRADFERIVTPVYDFLNDSPSRVPMTDWYFTDSGKQRGFQARPVVGGVFIKM
                     LSDDSMWRNWAGRAQKVTGEWAPMPTPPTVEVIEPGSKMDGVIWRYTTDRPADGWMSQ
                     TFNDHSWKKGPGGFGTNGTPGAMVRTRWNTPDIYIRREIIVPAGTDIAALQLYVHHDE
                     DATIYLNGVLAAQISGFTTDYDPVEIIPAAKAALKPGTNLLAVHCHQTVGGQYIDVGL
                     ARIKE"
     gene            complement(2680..>3179)
                     /locus_tag="VLI90_06865"
     CDS             complement(2680..>3179)
                     /locus_tag="VLI90_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSV13964.1"
                     /translation="RGEIFAYGLREPWRFSFDSATGDLWVGDVGQDRIEEVDIVRRGE
                     NYGWNVYEGFDLFATRYRSEGRQYVPPVFAYNRRLGNCVIGGYVYRAPGSPFDGIYVC
                     GDNSSKRVWGIRQSDRKLTGIWQLCTSPLEIASFARDEAGALYLVGYNGGTIYRLDFD
                     NAVLP"
BASE COUNT          541 a         1022 c         1012 g          604 t
ORIGIN      
        1 aggcgacgca ggcgtcgcag ctactagaca ggcacgtttt ggggcaagtc atgcacgttg
       61 cagcggcatt cgagccggtt actccttaat cctggctagc ccaacgtcga tgtactgccc
      121 gccgacggtc tggtggcaat ggacagccaa cagattcgtt ccgggtttga gcgcggcctt
      181 ggcggccggg atgatctcga ccgggtcgta gtcggtggtg aagccgctga tctgggcggc
      241 gaggacgccg ttcaagtaga tcgtcgcgtc ttcatcgtgg tggacgtaga gctgcagcgc
      301 tgcgatgtcg gtgccggcgg gcacaataat ctcgcggcgg atgtagatgt cgggcgtgtt
      361 ccatcgcgtg cgcaccatcg cgccgggcgt gccgttggtg ccgaacccgc cgggaccttt
      421 tttccagctg tggtcgttga acgtctggct catccaacca tcggccggtc gatcggtcgt
      481 gtagcgccag atgacgccgt ccatcttcga gccgggctcg atcacttcca ccgtcggcgg
      541 cgtgggcatc ggggcccatt cgcccgtgac tttttgcgct cggccggccc agttccgcca
      601 catcgagtcg tccgacagca tcttgatgaa gaccccaccg acgactgggc gggcctggaa
      661 cccgcgctgc ttgccgctgt cggtgaagta ccaatccgtc atcggcacgc gcgacggtga
      721 atcgttcagg aagtcgtaca ccggcgtgac gatccgctcg aaatccgcac gccgatcggc
      781 cagcgtcgcg gtccagatcg tccagtccag cttcgtgtac gtgctgcgcg aatccagcgg
      841 caggccgtac ttgttctgct tcgtcaggta gaacgcgatt tccttttgtg cgaccgattt
      901 cgggaacagg ttcaggccca ggactttgtc ccagaccagg ttgtacttct ggctccaggt
      961 gccgcgcttg tcgaaggtga gccgggaatg atcgccgtcg gcggcctcgg tcacccagcg
     1021 gtcggcgaag cttcgggcga tcgcgcgcac ccgcgcggct tcggttttgt cgccgcgcgt
     1081 ctcgcatagc atcgaatatg aaccgagccc catgatggcc ttgatcgaca ggttggtgtt
     1141 gtgagccagg tgaccggcga agtcgtcggt gcacaactgc ttctccggat cgaatccctt
     1201 cgcttccaga tatttcgccc agcgcgtgat ggccggccag tacttgccgg cgaagtcggc
     1261 gttgccatca gccttggcga cggcggccag cagcgccagc atgttgccgg attcttccac
     1321 cggcatctgg ttgtcttcgg tgcgctcgcc cccgccgtag acctggccat tcgcttgtgg
     1381 gtaggtgccg agatcgtgcg gggcgaaatc gaagtgccag cgatcagacg acgcgtagtc
     1441 cagcagcggg acgagcgccg ccttcgccag cgcagggctg gtcagcagga gctgcggcag
     1501 ctgcggatag atcacatcga ccgtggcgat gcagccgttg gaaaagttct ccttgcagaa
     1561 cgccagcggc attccgttgg cgtcggcgac tatcttttgc gctgccagcg cctggcgata
     1621 tgccagcgcg caaatccgcg catacgcctc gccgccggtg ctgcgcgcat cgccgatcag
     1681 atcgtcgtcg aacttcgcgc atcgctcggc gagattggca tattcggctt cggccgtttg
     1741 cagcagcctc cccgcgtcca tgccgttgcg ccgccagtac ggtcgcaggt tgcgcttcat
     1801 gtactggatc gaaaacagat cgtcgtatgc gaccatcgcg tgacgcgcga cgttttgatt
     1861 gccgacggcg ccgagatcga cgacgaccgc ggccgacggc aggccgtctc gcaccgggcg
     1921 cggaaagcgc gtgtcgtcgc tggccggcag gttgccgctg tcggcgaacg tgctcgccag
     1981 cacgcccgcg ttgccgatcg ccatccccgc ggcatcgcgc gcggtgacgt agaggtaacc
     2041 ccagtcgatg cgatgatcgt cgcccttgct tcgaaggatt ggttgcgctt cgctaccgat
     2101 cgacatcgcg gtgagcgtgc cgaacgtctg gcgatgcgcg accaccgctt catccggcgt
     2161 gttcacagca atgtccccgc ccatcgcaag catcgccgac acctggtgcg attgcccgtc
     2221 gctggcgacg gcgttccacg tcacgtacgt cagcggccga gagagcacat ccagatcaac
     2281 gggcaacatc ggcgtggtga acgtcagcgt cagcgtgacg ccgccgccgg tgaacgtgta
     2341 gaacgtccgc gtcggcagca cgagcaggtt cgtctgctgc atcgccggcg catcgctcgg
     2401 ctgcgcgccg gccaatcgat atgccttgcc gtcgatccgc acgaggctga gcagcgtctg
     2461 cggcttgccc gtccaatgat gcgtgtcggc gtcgttcaag tgatcgtcga atgaccagat
     2521 gctgaagtag ggatcggtcg tcaccagcgg caccgccggg ggacgcaacg ggggaagatc
     2581 agtgtcggcc gcacgcgccg gcaagccatg ggcgaacagc gccgccgctg ccgtcatcac
     2641 caggatcatc acagattggt tcacagttcc tcctcaatgc taaggcaaaa cggcgttgtc
     2701 aaaatcgagc cgatagatcg tcccgccgtt gtatccgacg aggtatagcg cgcccgcttc
     2761 gtcgcgcgcg aacgaagcga tctccaacgg cgacgtgcag agctgccaga tgcccgtcag
     2821 cttgcgatcc gactggcgga tgccccacac gcgcttggac gaattgtcgc cgcacacgta
     2881 gatgccgtcg aacggcgaac cgggcgcgcg atagacgtat ccgccgatca cgcagttgcc
     2941 caaccggcgg ttgtaggcaa acaccggcgg cacatactgc cggccttcgc tgcgatatcg
     3001 cgtcgcgaat aaatcgaagc cttcgtagac gttccaccca tagttctcac cgcggcggac
     3061 gatgtctact tcctcgatcc ggtcctgccc gacatcgccg acccacaggt cgccggtcgc
     3121 ggaatcgaag ctgaaccgcc agggctcgcg caaaccatag gcgaagattt cgccgcgcg
//
LOCUS       DATIMH010000398         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4134842, whole genome shotgun sequence.
ACCESSION   DATIMH010000398 DATIMH010000000
VERSION     DATIMH010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4134842"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VLI90_06870"
     CDS             <1..206
                     /locus_tag="VLI90_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13965.1"
                     /translation="TQRHADSSAIPLAAADFAQGGLKMNSVVRPGKLFTANHAIVRKR
                     VARVKDVTRDLIRDAVVRVIRSG"
     gene            227..388
                     /locus_tag="VLI90_06875"
     CDS             227..388
                     /locus_tag="VLI90_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13966.1"
                     /translation="MFRHDKSDLRASARPPYFGPRMKWTILVVVLVCVVSYLVQHWWT
                     AWVKQGLGW"
     gene            513..800
                     /gene="groES"
                     /locus_tag="VLI90_06880"
     CDS             513..800
                     /gene="groES"
                     /locus_tag="VLI90_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSV13967.1"
                     /translation="MKLKPLADRVIVRQSDAEEKTKSGIVLPDSAKEKPTKGKVISVG
                     PGKFDDNGKRMELGVRVNDTVYYGKYSGTDVEVDGQKFVILRESDILGVLE"
     gene            897..>2250
                     /gene="groL"
                     /locus_tag="VLI90_06885"
     CDS             897..>2250
                     /gene="groL"
                     /locus_tag="VLI90_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSV13968.1"
                     /translation="MAAKQLMYETDARAELLAGVSQLARAVKATLGPRGRNVVLQKSF
                     GSPRITKDGVTVAKEIELPQPFENMGAKLVAAVASKTGDVAGDGTTTATVLAEAILSE
                     GLKFVRTGVNPVQIQRGILKASEVAAEAITNISKKVKGHEDYKRVATISANGDEHIGQ
                     LMADAMEKVGKEGVITVDEGKGVTSELEYTEGMQFDKGYLSPYFMTNPTTLEAIQETP
                     YILLHEKKINNLAELLPLLNKIVGGGRPLLIIAEDVEAEALAALVVNKLRGVLNVCAV
                     KAPGFGDRRKAMMGDLAAVTGGTFISEDLGLKLENIELDQLGSAKRVVINKDNTLIVE
                     GAGSKKEINGRVEQIRKQVETTTSDYDREKLQERLAKLTGGVAVVKAGANTETEMKER
                     KDLIDDALHATRAAAEEGIVPGGGVAFLRAIEAVENAKRQAKGDEKFGFDILAEALRA
                     P"
BASE COUNT          510 a          627 c          758 g          355 t
ORIGIN      
        1 cgacgcagcg acatgcggat tcgtctgcga ttccattggc ggcggcggat ttcgctcaag
       61 gcggtttgaa aatgaacagc gtcgtccgcc ctggaaagct gttcacagcg aaccacgcga
      121 tcgtgcggaa gcgagtcgcc cgtgtcaaag acgtgacgag agacttgatt cgagatgcgg
      181 ttgtgcgagt gatccgcagc ggctgatcgc tacggtggaa gatgtgatgt tccgccatga
      241 taagagcgac ctgcgagcct cggcgcggcc gccgtatttc gggccgcgga tgaagtggac
      301 gatcctcgtc gtcgttctgg tctgcgtggt gagttatctc gtgcagcact ggtggacggc
      361 gtgggtgaag cagggactgg gatggtaggg acgagcgaat gaatcaatga agccccgcat
      421 gtacatgcgg ggctccggtg aagcgaacgt atacacaggt gacgcgcggc cgcgtcgcca
      481 tttgatctgg taagcgaaag aggtaacgca acatgaaact caaacccctg gccgatcgcg
      541 tgatcgtgcg gcagtcggac gccgaagaga agaccaagag cggcatcgtg ctgccggact
      601 cggcgaagga aaagcccacc aaaggcaagg tcatctccgt cggtcccggt aagttcgacg
      661 acaacgggaa gcgcatggag ctcggcgtcc gcgtgaacga caccgtctac tacggcaagt
      721 acagcggcac cgacgtggaa gtcgacgggc agaagttcgt catcctgcgc gaatcagaca
      781 tcctgggcgt gcttgaatga gttgctagtt gctggttgct agttgctagt gaatgcaacg
      841 gtgaccggca ttttctagca actagcaaca aacaactagc aactaatgga gttctcatgg
      901 cagcgaaaca actgatgtac gaaaccgacg cccgggcgga attgctcgcc ggcgtgtcgc
      961 aactggcgcg ggccgtcaag gcgacgctgg ggccgcgcgg tcgtaacgtg gtgttgcaga
     1021 aatcgttcgg cagcccgcgg atcaccaagg acggcgtgac ggtcgccaag gagatcgagc
     1081 tgccgcaacc gttcgagaac atgggcgcaa agctcgtcgc cgcggttgcc agcaagacgg
     1141 gtgacgtcgc cggtgacggc acgaccaccg caactgtgct ggccgaggcg atcctcagcg
     1201 aagggctgaa gttcgtccgc accggcgtca atccggtgca gatccagcgc ggcattctca
     1261 aggcatccga agtcgccgcc gaggcgatca ccaacatctc caaaaaggtg aagggtcacg
     1321 aggattacaa gcgcgtcgcg acgatcagcg ccaacggcga tgagcacatc ggtcagctga
     1381 tggctgacgc gatggagaag gtcggcaagg aaggcgtgat cacggtggac gaaggcaagg
     1441 gcgtgacgtc cgagctggag tacaccgaag gcatgcagtt cgacaagggt tacctgtcgc
     1501 cgtacttcat gaccaacccg acgacgctcg aggcgatcca ggaaaccccg tacatcctgc
     1561 tgcacgagaa gaagatcaac aacctggccg agctgctgcc gctgctgaac aagatcgtcg
     1621 gcggcggtcg cccgctgctg atcatcgccg aagacgttga ggccgaagcg ctggccgcgt
     1681 tggtcgtaaa caagctgcgc ggcgtgttga acgtgtgcgc ggtgaaagcg ccgggctttg
     1741 gcgatcgccg caaggcgatg atgggcgatc tcgccgccgt caccggtggc acgttcatca
     1801 gtgaagacct gggcctgaag ctggagaaca tcgagctgga tcagctcggc agcgccaagc
     1861 gcgtcgtcat caacaaggac aacacgctga tcgtcgaagg ggctggcagc aagaaggaga
     1921 tcaacggccg cgtcgagcag atccgtaagc aggtggaaac gacgaccagc gattacgacc
     1981 gcgaaaagct gcaggaacgc ctggctaagc tgaccggcgg cgtggccgtg gtgaaggctg
     2041 gcgcgaatac cgaaaccgaa atgaaggagc gcaaggacct gatcgacgac gcgctgcacg
     2101 cgacgcgcgc cgcggccgag gaaggcatcg tccccggcgg gggcgtcgca ttcctgcggg
     2161 ccatcgaagc ggtcgaaaac gccaagcgcc aggccaaggg cgacgagaag ttcggcttcg
     2221 acatcctggc agaagccctg cgtgccccgg
//
LOCUS       DATIMH010000399         1839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_382983, whole genome shotgun sequence.
ACCESSION   DATIMH010000399 DATIMH010000000
VERSION     DATIMH010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382983"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..>1839
                     /locus_tag="VLI90_06890"
     CDS             55..>1839
                     /locus_tag="VLI90_06890"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSV13969.1"
                     /translation="MRSILMRSMACGSGSSEYLRTRRGWAILLSLVVMLAGSAARGST
                     SPETRPTTRPTTLIEQKDCVTSKCHANVKSTAVLHGPVATDNCDACHSLNDVRKHTFT
                     VPRTKAELCTYCHEFSVAAMPVVHKPVTQGECLGCHDPHGGRTKAIVREASTAELCGR
                     CHESITRNKSFLHGPVAKGECDSCHPPHASRFPKLLDVVGTDMCLTCHVAFEPAVSGA
                     RSQHKAMEKGCEKCHDVHGSNQPMALLQPAQDLCLGCHDKLKAKIDAATVKHSVVTKD
                     RACLNCHASHASNVGKLLREPSAVSCLKCHTKEVTRSDGVTIAAVPEIADPTTEKHGE
                     IRDGQCGGCHDVHGGTRSNLLVKPYARQLYQRFQPDDYALCFGCHDMQPFMQRRTIKQ
                     TNFRDGEVDLHYLHANRGHLDKNCRACHLTHAGPNPRIIRTELAFGKWVMPMRFKKTA
                     TGGSCFPGCHRELAYDRENPVASTTAPTSGATTEPATPAIARAQQEDTQKIHWSATDI
                     GGASVTIPDGRRATALVMLSDDDNAGNDAVVAQLRSALPAETDAQVTIIISGKTAASA
                     ATTMAVKKATGWSIIADADHAAAEALGVR"
BASE COUNT          366 a          578 c          623 g          272 t
ORIGIN      
        1 gacgactcgc gcttcaacca aacccacgat cattcgtatt cggcgaggtg ggtcatgaga
       61 tccatcctca tgcgcagtat ggcgtgcgga tccgggtcgt cggagtacct ccgaacccgg
      121 cgtgggtggg cgattctgct atcgctggtg gtcatgctgg ccggcagcgc tgcgcgcgga
      181 tcgacgtcac cggagactcg gccgacaact cgcccgacga cgctgatcga acagaaggac
      241 tgcgtcacat ccaagtgcca cgcaaacgtc aagtcgaccg cggtgctgca cgggccggtc
      301 gcgaccgaca attgcgatgc gtgccattcg ttgaatgatg ttcgcaagca cacgttcacg
      361 gttccccgga ccaaggcgga gttgtgcacg tactgtcatg agttctccgt cgcggcgatg
      421 ccggtcgttc acaagccagt cacacagggg gaatgcctgg gctgccacga tccccacggc
      481 gggcggacca aggcgatcgt gcgcgaagcc agcaccgccg aattgtgcgg ccgatgtcac
      541 gagagcatca cgcggaacaa gtcgttcctg cacggccccg tggccaaggg cgagtgcgat
      601 tcgtgccacc cgccgcacgc gtcgcggttt ccgaagctgc tggacgtggt gggcacggac
      661 atgtgcctga cgtgtcacgt cgcgttcgag ccggcggtgt cgggcgcgcg gtcgcagcac
      721 aaggcgatgg agaagggctg cgaaaaatgc cacgacgtgc acggatcgaa ccagccgatg
      781 gcgctgcttc aaccggcgca ggatttgtgc ctgggttgtc acgataagct gaaggcgaag
      841 atcgacgcgg cgacggtgaa gcattcggtg gtgacgaagg atcgcgcctg cctgaactgc
      901 cacgcatcgc acgccagcaa cgtcggcaaa ttgctgcgcg aaccgtcggc ggtcagctgt
      961 ctcaagtgcc acaccaaaga ggtgacgcgc agcgatggcg tgacgatcgc cgccgtgccg
     1021 gagatcgccg acccgacgac cgagaagcac ggagaaatcc gcgacggcca gtgcggcggg
     1081 tgtcacgacg tgcacggcgg cacgcgatcg aacctgctgg tcaagccgta cgcgcgccag
     1141 ctgtaccagc gatttcaacc ggacgattac gcgttgtgct tcggctgtca cgacatgcag
     1201 ccgttcatgc agcggcggac gatcaagcag accaacttcc gcgacggcga ggtggacctg
     1261 cattacctgc acgccaaccg cggacacctg gacaagaatt gccgcgcgtg ccacctgacg
     1321 cacgcggggc cgaacccgcg gatcattcgc accgaactgg cgttcggcaa atgggtgatg
     1381 ccgatgcggt tcaagaagac ggcgaccggt ggcagctgct tcccgggatg tcatcgcgag
     1441 ctggcgtatg accgcgagaa tcccgtcgcg tccaccaccg cgccaacgag cggtgcgacg
     1501 acggaaccgg cgacgccggc gattgcccgc gcgcagcagg aggacacgca gaagatccac
     1561 tggagcgcta cggacatcgg cggcgcgagc gtgacgattc cggacgggcg tcgcgctaca
     1621 gcgctggtga tgctcagcga cgacgacaac gccgggaacg acgcggtggt cgcgcagctt
     1681 cgcagcgcat tgccggcgga gacggacgcg caggtcacca tcatcatcag cggaaagacc
     1741 gcggcatcgg cggcaacaac aatggcggtg aagaaggcga cgggctggtc gatcatcgcc
     1801 gacgccgatc acgccgccgc cgaggcgctg ggcgtgcgc
//
LOCUS       DATIMH010000400         4342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_960708, whole genome shotgun sequence.
ACCESSION   DATIMH010000400 DATIMH010000000
VERSION     DATIMH010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960708"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..958
                     /locus_tag="VLI90_06895"
     CDS             263..958
                     /locus_tag="VLI90_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HSV13970.1"
                     /translation="MRAYLTEFIGTFFLVLTIYLAVSSGSPLAPLAIGASLMVMVYMG
                     GHISGAHYNPAVSLAVMFRGKLPPVEFLFYVIAQLFGATVAALMGASITHHEFAPAPG
                     VGVGWGAALMVEILYTFALALVVLNVATAPKTEGNSYYGLAIGFTVAAGAFAGGGISG
                     GAFNPAVGVGPILIDTIHGGHSMPHLWLYIVGPCIGGFVAAFIFRYQNEAALPAVPTA
                     APAGPKEAIAPEL"
     gene            complement(972..1913)
                     /locus_tag="VLI90_06900"
     CDS             complement(972..1913)
                     /locus_tag="VLI90_06900"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV13971.1"
                     /translation="MTSTTPNFQPTRPLGRTGFLATALGIGDVADRALPIEQCVATAR
                     RAIDTGLNLIDTAPNYEDGYSEQIVGRAVKGVRDRMFVISKIDHFDAPIEPQVDGSLG
                     RLQMDHVDLFVFHGLSTIDAWEKAAAPGGAFDALARCAKAGKLRFRGISSHDPEVLRV
                     AMPSGLCDVVMFAIGPYVDPRYVDEILPLAKQHGVGTVCFKTFGAGKLLGDTSGYQRP
                     LAQRPRGKISSGGAEANDAVLPRLTVKQCLHYTLSVGPDVALLGMSFPNEQDAAFAAA
                     RSFKQPIAADELAEIRRRAAVAIADKGPCWWNPSPGA"
     gene            complement(2138..3028)
                     /locus_tag="VLI90_06905"
     CDS             complement(2138..3028)
                     /locus_tag="VLI90_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HSV13972.1"
                     /translation="MTRHMDIEQRRSSNDGGGDSDSDEKEKKDREREEDEVHEKAAAG
                     AHIVYKAILKEADEELARPSIALAWSGLAAGLSMGFSLVAEGLLRAHLPESPWRSLVA
                     KLGYSIGFLIVILGRQQLFTENTLTPILPLLKRRDAGTFANVMRLWIVVLLTNLIGAA
                     LFAYAVQRTSAFEPQVKETFRLLGMEAMEPPFRHLLVKGIFAGWLIALMVWLLPVAET
                     GRISVIIIITYIVGLGQLSHIIAGSVEALYTVAGGEHSWGEFLGHYALPTLIGNCIGG
                     VTLVAALNYAQVAAGGDDEE"
     gene            complement(3128..3994)
                     /locus_tag="VLI90_06910"
     CDS             complement(3128..3994)
                     /locus_tag="VLI90_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSV13973.1"
                     /translation="MSEPASIACATHIGAVALTVADAQRIAAFYRDVLGFRVHLEAAS
                     QIALGAGGPDLLILHENPQAPSPPAHSTGLFHLAILLPSRAALAQSLRQLAQSRTPLQ
                     GASDHLVSEALYLADPEGNGIEIYRDRPREQWLMRNGQLQMSTDPLDIESLMGELAQH
                     DEPWPGLPPLTRIGHVHLRVADIPAAEAFYHGVLGFNITTRYGPAASFLSAGGYHHHI
                     GVNTWSSRGAAPPPPGARGLRHFTIQLPDEAERDRTVARLIASGHKVQRMPDGPFVRD
                     PSGNALLLLANS"
     gene            complement(4114..>4342)
                     /locus_tag="VLI90_06915"
     CDS             complement(4114..>4342)
                     /locus_tag="VLI90_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13974.1"
                     /translation="HGRDARATVTHMTQSTGGNRERIAALMKQLQTPGLDQAKRDQTL
                     DELQDLVGLAGKMVMEQDIVRRAKKIVEGNA"
BASE COUNT          739 a         1422 c         1378 g          803 t
ORIGIN      
        1 ggcatgaact caggtaaaac gagccggcac gaccgcatgt gacgcgcgcc ctctggtccc
       61 ctctcccggt acgccgggag agggttaggg tgagggtgat ttcgtttacg aaagtcgttc
      121 gacgttcgaa atcaccctca ccccgaccct ctcccggcgt accgggcgcg ggggaaagag
      181 ctaaacccga cctccttcga agccgccgtt gcattcgcga caaaaagttg atccgcatcc
      241 gccgcaccga tagcctcatg ttatgcgcgc ttatctcacc gaattcatcg gcaccttttt
      301 tctcgtcctc acgatctacc tcgccgtcag cagcggatcg cccctcgccc cgctggcgat
      361 cggcgctagc ctgatggtga tggtctacat gggcgggcat atctccggcg cgcattacaa
      421 cccggccgtt tcgctggcgg tcatgtttcg cgggaagctg ccgccggtgg agttcttgtt
      481 ctacgtaatc gctcagttgt tcggcgcgac ggtggccgcg ctgatgggcg cttccatcac
      541 gcatcacgag ttcgcccccg cgccgggcgt tggcgtcggc tggggcgctg cgctgatggt
      601 cgagatcctc tacaccttcg cgctggcgct ggtcgtgctg aacgtcgcca ccgcgcccaa
      661 gaccgaaggc aattcgtact acggcctggc gatcggtttc accgtcgccg ccggggcgtt
      721 cgccgggggt ggcatcagcg gcggggcgtt caacccggcc gtcggggtcg ggccgattct
      781 gatcgacacg atccacggcg gccactccat gccgcacctg tggctgtaca tcgtcggccc
      841 gtgcatcggc ggattcgtcg cggcgttcat cttccgatat cagaacgaag ccgcgttgcc
      901 cgccgtgcca accgccgccc ccgcggggcc gaaggaagcg atcgcgccgg aactctgatt
      961 cgacgaaacg atcacgcccc gggcgacggg ttccaccagc acggtccttt gtcggcgatc
     1021 gcgacagccg cgcgccggcg gatctcggcc aactcatcgg ctgcgatcgg ctgtttgaat
     1081 gatctggctg ccgcgaaggc agcgtcttgt tcgttgggga aactcatccc cagcagcgcc
     1141 acgtccgggc cgacgctcag cgtgtaatgc aggcattgtt tcaccgtcag gcgcgggaga
     1201 acagcgtcat tcgcctcggc gccgccggag ctgatcttcc cgcgcgggcg ctgcgccagc
     1261 gggcgttgat agccgctggt gtcgccgagc agcttgcccg cgccgaaggt cttgaagcag
     1321 accgtgccga cgccatgctg cttcgccagc ggcaggattt catcgacgta gcgcggatcg
     1381 acgtacggcc cgatggcgaa catcacgaca tcgcacaagc ccgagggcat cgcgacgcgc
     1441 agcacttccg gatcgtgcga tgagatgccg cggaagcgca gcttgccggc tttggcgcag
     1501 cgcgccagcg catcgaacgc gccgccgggt gccgccgctt tctcccaggc gtcgatcgtc
     1561 gaaagcccgt ggaagacgaa caggtctacg tggtccatct gcagccggcc caggctgcca
     1621 tcgacctgcg gctcgatcgg cgcatcgaaa tggtcgatct tgctgatgac gaacatgcga
     1681 tcgcgcacgc ctttgacggc ccggccgacg atctgctcgc tgtagccgtc ctcgtaattg
     1741 ggcgccgtgt cgatcaggtt cagtcccgtg tcgatcgcgc gacgcgcggt cgcgacgcac
     1801 tgctcgatcg gcagcgcccg atcggcgacg tcgccaatgc ccaaggcggt ggcgagaaac
     1861 ccagtgcgac cgagcgggcg agttggctgg aagttcggcg tggtcgaggt catcgcttca
     1921 ttttatcgca atacgccagc ggtcaatgac caaggctcaa actccaaacg ccaatggagt
     1981 tccaatgacc aaagcccaag cagcgcgcaa ttgagctctg ggccttggga cttcattggg
     2041 atttggcttt tggacattgg tcatgcaaag ctccaaacca ccctcaccct aaccctctcc
     2101 cggagtaccg ggcgagggga ccagagcggt agctctccta ctcctcatca tcaccccccg
     2161 cggcgacttg ggcgtagttc agcgccgcca ccagcgtcac gccgccgatg cagttgccga
     2221 tcagcgtcgg caacgcgtag tggccgagga attcgcccca gctgtgttca ccgccagcga
     2281 ccgtgtacag cgcctcgacc gagccggcga tgatgtggct cagctgcccc agtcccacga
     2341 tgtacgtgat gatgatgatc accgaaatcc gcccggtctc ggcaaccggc agcagccaga
     2401 ccatcagcgc aatcaaccaa ccggcgaaga tgcccttgac cagcagatgc cggaacggcg
     2461 gctccatcgc ttccatcccc agcaggcgaa acgtttcctt cacctgcggc tcaaacgcgc
     2521 tcgtgcgctg cacggcgtag gcgaatagcg ctgcgccgat caggttcgtg agcagcacga
     2581 cgatccacag gcgcatgaca tttgcaaacg tgccggcgtc acgccgcttc agcagcggca
     2641 ggatcggcgt gagcgtgttt tccgtgaata attgttgccg tccgaggatc acgatgagaa
     2701 agccgatgct gtatccgagc ttggcgacca gtgaccgcca cggcgattcg ggcagatgcg
     2761 cgcgaagcag tccctcggct acgagcgaaa accccatcga cagccccgcg gccaagccgc
     2821 tccacgccag cgcgatgctc gggcgggcca gctcttcgtc ggcttccttg aggatcgcct
     2881 tgtaaacgat gtgcgccccc gcggcggcct tttcgtggac ctcgtcttct tcccgctcgc
     2941 gatccttctt ttccttctcg tcgctgtcac tgtcgccgcc gccgtcgttg gaactgcggc
     3001 gttgctctat atccatgtgt cgcgtcacgg gcgaacctcc cttatcgcgg cgcgccagag
     3061 gagcgcgaac gcgtggtatc gttcagcttg aacaacgcga ggaatttggg gaaccgcgaa
     3121 tatgcgatca ggaattcgcg agaagcagca aggcattccc cgacgggtcg cgcacgaatg
     3181 ggccgtctgg catgcgttgg actttgtggc cgcttgcgat gagccgcgcg acggtgcggt
     3241 cgcgctcagc ttcatccggc agttggatcg tgaagtggcg caacccgcgc gcgcccggcg
     3301 gcggtggggc ggcgccgcgg ctcgaccagg tgttcacacc gatgtgatgg tgatagccac
     3361 cggcggacag aaacgacgcg gcggggccgt aacgggtcgt aatgttgaag ccgagcacgc
     3421 cgtgatagaa cgcctcggcc gcgggaatgt cggcgacgcg caggtggacg tgaccgatgc
     3481 gagtcagcgg cggcaggccg ggccatggct catcgtgctg cgccaactcg cccatcagcg
     3541 attcaatatc cagcggatcg gtgctcatct ggagctgccc gttgcgcatc agccactgct
     3601 cgcgcggccg atcgcggtag atctcgatgc cgttcccctc cgggtcggcg agatatagcg
     3661 cctcactcac gagatgatcc gaggcgcctt gaagcggcgt tcgactctgc gcgagttgtc
     3721 gcaggctttg tgccagcgcc gcccgcgatg gcagaaggat cgcgaggtga aagaggccgg
     3781 tggaatgcgc gggtggactg ggggcttgtg gattctcgtg caggatgagc aaatcgggtc
     3841 cgcccgcgcc gagcgcgatc tgcgacgccg cttcgagatg cacgcgaaag ccgagaacgt
     3901 cgcgatagaa ggccgcgatg cgctgggcat ccgcgacggt cagcgcgacg gcgccgatat
     3961 gcgttgcgca cgcgatggat gccggttcgc tcatcgtggg gcgtccatca tagccgcagg
     4021 cgacagccag cgggggcgct ggataaatca tctacgctgg tactcgagcg cccccgcggg
     4081 ctgtcgcctg cggctattaa agatggcggt cgttcacgcg ttgccttcga cgatcttctt
     4141 cgcccgccgc acgatgtctt gctccatcac catcttcccc gccaggccaa ccaggtcttg
     4201 cagctcatcc agcgtctggt cgcgcttggc ctgatccagt cccggcgttt ggagttgctt
     4261 catcaacgcc gcaatgcgtt cgcgattgcc gcctgtggat tgcgtcatgt gggttaccgt
     4321 agcacgggcg tctcgcccgt gc
//
LOCUS       DATIMH010000401         4262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2114159, whole genome shotgun sequence.
ACCESSION   DATIMH010000401 DATIMH010000000
VERSION     DATIMH010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114159"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VLI90_06920"
     CDS             complement(<1..1052)
                     /locus_tag="VLI90_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13975.1"
                     /translation="MQVVLWAIFAGTVGLAELVTRERTHTARAELSQARTIDHLTLRL
                     PGKWIVSNGADGSNTLLVAHEPASGLGHGEGRTLTLSRQRARGASSTQEFLLQSGLLH
                     GALSMGEDADPDGEDGGGPVAPAPAEAAAMQPLKMGGWPAVMTTVYRPHISPMGFSAS
                     LHKQVIAAALLPSHQAVVLTLESAMALQPTAEFPDDSAGGEDAVMVRQIAEGMRIDGE
                     QSWQKESTLTLSSGLSVTLPDGWLLSPAVDSNRTRRAIALERDNSMRVEINPCVLLPD
                     DGPDVVRSMLLASHPELCGAKLTRDDAGRWHAQWPGASAMGSRASGARAIGRADGNSV
                     LLVFFGAPIESGDFNDA"
     gene            1483..2856
                     /locus_tag="VLI90_06925"
     CDS             1483..2856
                     /locus_tag="VLI90_06925"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HSV13976.1"
                     /translation="MDLFRESRQKNLDRVAPLAVRMRPRSLEEFVGQQHFLGPGKLLR
                     RMLEADRLTSVIFYGPPGTGKTTLAQLIATYTKSHFDQVNAAAVGVKDVRAILDNAKE
                     RLANGGQRTVLFLDEIHRFNRAQQDILLPDVEAGLVLLVGATTENPFFAVNSPLISRS
                     QIFQFAPISEDDIRALIRRAIADPERGYGKLNITIDEDAIKLWATMSDGDARRALMAL
                     EVAVLSLQKESSTAVSAVSNDAGHRRDARATSIHVNLDVAEQSIQRKAIVYDGTGDEH
                     YDAASALIKSMRGSDPDAAVYWVARMLEAGEDPRFIARRIAILASEDIGNADPNAIQV
                     AAAAFDIVEKVGMPEAQLTLGQAAIYMATAPKSNASALAIWSAMSDVREGRTLPVPKH
                     LKDTHYKGSKRLGHGEGYKYAHDFEGGHVEQDYLGVDKTYYTPTDRGYEAEIQRRIAE
                     RKPRPAE"
     gene            3431..>4262
                     /locus_tag="VLI90_06930"
     CDS             3431..>4262
                     /locus_tag="VLI90_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13977.1"
                     /translation="MLLRWLKHGTADGERRAIAAAASLCLFLWTLKVHLVWWMWWSKG
                     VHVLADVPAATVPLLGAHDLLLCAGLAVIYALVAMLARPLPRGLGQAIAYGVPALIGC
                     AVALFAVISWKVQQLYGCAVEMDHFRAATGISAMRDSIVAYLTAGTIAFLIMAVLTPT
                     LGAYLLARVRWSPPRWRTWFVLLGGSVALYGADKSLMKERYTHGVEQDAVLHFVQWYE
                     PAPRPVDVAEMLRDTPAQVPSRDAEFRRFTPFAPMPQQLGASDQTPTTAGLPLVAPQP
                     T"
BASE COUNT          766 a         1366 c         1366 g          764 t
ORIGIN      
        1 gcgtcgttga agtcgccgct ttcgatcggc gcgccgaaga acaccagcag cactgaattc
       61 ccatcggctc gcccaatcgc gcgcgcccca ctggcgcgcg accccatcgc gctggcgccc
      121 ggccattgcg cgtgccagcg cccggcgtcg tcgcgagtca gcttcgcccc gcacagttcc
      181 gggtgactcg ccaacagcat cgatcgaacg acgtcgggtc cgtcgtctgg caacagcacg
      241 cacgggttga tctcgacgcg catcgagtta tcgcgctcca gcgcaatcgc ccgccgggtg
      301 cgattgctat ccaccgccgg cgagagcaac cacccatccg gcaacgtcac ggatagcccg
      361 ctggacaacg tcagcgtgga ttctttctgc cacgactgtt cgccgtcaat gcgcatcccc
      421 tcggcgatct gccggaccat caccgcgtct tcaccgcccg cgctgtcatc ggggaactcc
      481 gccgttggct gcagcgccat cgcggactcc agcgtcagca cgaccgcctg gtgcgacggc
      541 aacaacgcgg ccgcgatcac ctgcttgtgc agcgaagcgc tgaaccccat cggcgaaatg
      601 tgcgggcgat aaaccgtcgt catcaccgcc ggccaaccgc ccatcttcag cggctgcatc
      661 gccgccgcct ccgcaggcgc cggcgcaact ggcccaccgc cgtcctcgcc gtcggggtcc
      721 gcgtcctcac ccatcgacag cgcgccgtgc agcaagccgc tctgcagcag gaattcctgc
      781 gttgacgaag cgccgcgggc gcgctgccgg ctgagggtca acgtgcggcc ttcgccatgc
      841 cccaggccag acgccggttc atgcgccacc agcagcgtgt tgctgccgtc ggcgccgttg
      901 ctgacgatcc acttgcccgg cagccgaagc gtgaggtgat cgatcgttcg cgcctgcgac
      961 agctccgcgc gcgccgtgtg ggtgcgctcg cgcgtgacca gttcggccaa gccgacggtg
     1021 cccgcgaaga tggcccacag gaccacctgc atcaaaatcc gccgccatcg ggcattttgc
     1081 caggagcgag ccatcgtgcg gcagattgta ccgatcgaaa cgcgaatgtg cttgccgcat
     1141 gaccaggacg aacggatgtg aagattactc gctctggtgc cctctctcgg tacgccgggg
     1201 ctgacgaacc gacacttttg caggaatggt tggcaaccga ccacgccggg ttcggaggta
     1261 ctccgacgac ccggattgtt cccgcgcgtc cagcagaaga atctcgcttc atcggagtac
     1321 ctccgaaccc ggcgtgggtc ggtcccttgc taagtctgca aaagtgtcgg gccgtcgagg
     1381 acgccgggag agggggaaga atcacttgag attgtcctgc cgcacgacgc gctcgtctat
     1441 tgcgccgtcg cgtccattgg cgttacgcgc taagatgtcc ccatggacct cttccgcgaa
     1501 agccggcaaa agaacctcga tcgcgtggcg ccgctggcgg tgcgcatgcg gccacggtcg
     1561 ctcgaagaat tcgtcggcca gcagcacttc ctcggtcccg gcaaactgct tcggcgaatg
     1621 ctcgaggcgg atcgcctgac cagcgtgatc ttctacggcc cacccggcac cgggaaaacg
     1681 acgctcgcgc agctcatcgc gacgtacacc aaaagccatt tcgaccaagt gaacgcggcg
     1741 gccgtcggcg ttaaggacgt ccgggcgatc ctggacaacg ccaaggaacg cctcgccaac
     1801 ggcgggcagc ggacggtttt atttctggat gagatccacc gtttcaatcg cgcgcagcag
     1861 gacatcctgc tgccggacgt ggaagcggga ttggtgttgc tggttggcgc aacgacggag
     1921 aacccgttct tcgcggtcaa ctcaccgctg atcagccgga gccagatctt ccagttcgcg
     1981 ccgatcagtg aagatgatat tcgcgcgctg attcgacgcg cgatcgccga tccggaacgc
     2041 ggctatggga agttgaatat cacgattgat gaagatgcaa taaaactgtg ggcgacgatg
     2101 agcgatgggg atgctcggcg agcattaatg gcattggaag ttgccgtgtt atcgctgcag
     2161 aaagaaagta gcacggccgt ctcggccgtg tcgaacgatg cgggacaccg gcgggatgcc
     2221 cgtgccacat cgatccacgt caacctcgat gtcgccgagc aatcgatcca gcgcaaggcc
     2281 atcgtctacg acggcaccgg cgacgagcat tacgacgcgg cgagcgcgct gatcaagtcg
     2341 atgcgcggat ccgatccgga cgccgcggtt tactgggtcg cgcgcatgct cgaagccggc
     2401 gaagacccgc ggttcatcgc ccggcggatc gcgatcctcg ccagcgagga catcggcaac
     2461 gccgatccca acgccataca agtcgccgcg gccgcgttcg acattgttga aaaggtcggc
     2521 atgcccgaag cgcagttgac gctgggacag gccgccatct acatggccac ggcgccgaag
     2581 tcgaacgcat cggcgctggc gatctggagc gcgatgagcg acgtgcgcga aggccgcacg
     2641 ctgccggtgc cgaagcatct gaaagacacg cattacaagg ggtcgaagcg cctgggccac
     2701 ggcgaagggt acaagtacgc gcacgacttc gaaggcgggc acgtcgagca ggattacctg
     2761 ggcgtggaca agacgtacta cacgccgacg gaccggggat atgaggcgga gatccaacgg
     2821 cggattgccg agcggaaacc gaggccggcg gagtaatgcc ggcatcacgc gtttcccgtt
     2881 tcatcgatga acctgccgac cacgccgggt tcggaggcac tccgacgacc cggatgtttt
     2941 gtgattttca aggagaagaa tctcgcttcg tcggagtacc tccgaacccg gcgtgttaat
     3001 gcacctgaac catctcgggc atcagcggtt ttgcgggccg agcagcttgg ggagtgagat
     3061 cgtcgccatg tctcctgcaa agtaagaccc aagcgcttat atcttgaacc gcaagcggct
     3121 ggcctaggac tcgaactccg cgatacactt cgcctaactt gcgtattttg acgccgaagc
     3181 cagtgatttc tgctgcgaac ttcacggaaa aacatttcgg attgatgagc ctttcgttag
     3241 aatgcggaat ccccgatgag tcgcgtctct ttgggacgtg catcgattgg ctgcagtggg
     3301 tgcggcgaag ttcatctcat tcgttgccgc ctgtatttgt gaatgtctgt ggcgaatgcc
     3361 gtcgcgcgat tgcgcgcggc ttggacgctc gtcgccgctc aatcgtggat caccacgccg
     3421 tcaggatcgc atgttgctgc gctggttaaa acatggcact gcagacggcg aacgccgcgc
     3481 gatcgcggcg gcggcttcgt tgtgtctgtt cctgtggacg ctgaaggtgc acctggtctg
     3541 gtggatgtgg tggagcaagg gcgttcacgt gctggccgac gtgccggctg cgacggtgcc
     3601 gttgctgggg gcgcatgacc ttctgctgtg cgcgggactg gcggtcattt atgccttggt
     3661 cgcgatgctg gcgcgaccgt tgccgcgcgg cttgggacag gcaatcgctt acggtgtgcc
     3721 agcgctgatc ggttgtgccg tcgcgctctt cgcggtgatc agctggaagg tgcagcagct
     3781 ctacggctgc gccgtagaga tggaccactt ccgcgccgcg accggcatca gcgcgatgcg
     3841 cgattcgatc gtcgcgtacc tgacggcggg cacgatcgcg tttctcatca tggccgtgtt
     3901 gacgcccacg ctcggggcgt acctgctcgc gcgggtgcgg tggtccccgc cgcggtggcg
     3961 cacctggttc gtcctgctcg gcggcagcgt cgcgctctac ggcgccgata agtcgctgat
     4021 gaaggagcgc tacacgcatg gcgtggagca ggacgccgtt ctgcacttcg tgcaatggta
     4081 cgaaccggcc ccgcggccgg tggacgtggc ggagatgctg cgcgatacgc ccgcgcaggt
     4141 gccgtcgcgc gatgcagagt tccgccgctt tacccccttc gcgccaatgc cgcagcagct
     4201 gggcgcgagt gatcagacgc caacgaccgc ggggcttccg ctcgtcgcgc cgcagccgac
     4261 at
//
LOCUS       DATIMH010000402         5407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2692454, whole genome shotgun sequence.
ACCESSION   DATIMH010000402 DATIMH010000000
VERSION     DATIMH010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2692454"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1447
                     /locus_tag="VLI90_06935"
     CDS             371..1447
                     /locus_tag="VLI90_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HSV13978.1"
                     /translation="MPSSFFEILESRLCLSAAPAAGNWDLYASFDFNTPPTAPTFVNT
                     LWGHTHFPGELESYQPANVSASNGVASLTARQESVGGMPFSSGIINTGGNTATGGSIS
                     PGFSFLYGYIEGRMKLVSGNGFWTGFWMLPTPNANGYHDGDGEIDIMEQIGSDPTSIA
                     GSLHHDPTNRTKDFHSGVDLSADFHTYGVDWEPDHITWYVDRSPYLTMTTDVPNVPMY
                     VILNLAIGDATSWAGTPDATTPFPTTMQVDYVHVYKSVPPAIAGDANIDGRVDVADLG
                     IVATNFGVTSGATWATGDFTADGRVDVADLGQFATNYGYGAQTFAASQITSQQIVKPQ
                     AADALDPDPLREYTKPRLAVIILP"
     gene            1444..2268
                     /locus_tag="VLI90_06940"
     CDS             1444..2268
                     /locus_tag="VLI90_06940"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSV13979.1"
                     /translation="MKERIQKVLGNAGVASRRNIEEMIRQGRISVNGRVATRLPVLID
                     PARDRVTVDDEPIKLAPAKDGGPGKAVGRRFYFLLNKPRGVYSTNVAQGAQVRAIDLL
                     PKSFGGRVYPVGRLDAESRGLLLLTNDGELTNQLTHPRYGVSKTYRAVVDGHVAPEDL
                     VKLEKGIWLADPHSGKGFKTGRSRIRIVKRARDKSILEITVREGRNRQVRRMLANVGH
                     KVRELTRIKMGPLTLDRLQPGAFRELTAREVKALRQLAERHEQTIASEAGDDVGEE"
     gene            2347..3591
                     /locus_tag="VLI90_06945"
     CDS             2347..3591
                     /locus_tag="VLI90_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13980.1"
                     /translation="MPISNPMNVVDRLRRFQRGVRDMIIRSRKSSGSMYEVSHSTVAD
                     TIYKIDTQVDPLLEGFCQEWGRETPLVLIAEGIEDEHGEEAAVTFPRGTREADAEIRV
                     IIDPIDGTRGIMYDKRAAWALAGVAPNKGPGTRLRDIEIAVMTELPTSKMGFADVLWA
                     IKGRGAHGVRESLNPSEVPLEDAVPIGRVLTKMGKVATEQVAEALHMQRTKGGLVGQI
                     LVGMGVVNESDVQIALAFQKGQKPDVGGEVATAIAGDALSLHSSQAVTINHGFASVSN
                     FFPGTKVLASELMEHLVRNLIGEADVTKATVFDDQYICTGGQFYELMIGHDRFNADLR
                     PTFYRMQGQPEGLCCHPYDCATMLIAEEAGVILTDERGDPLDGPLDTTTGLSWTGYAN
                     TELRRKVEPLVLEFLARASKKT"
     gene            3744..4136
                     /locus_tag="VLI90_06950"
     CDS             3744..4136
                     /locus_tag="VLI90_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV13981.1"
                     /translation="MSTILVVDDHADTRKPLVRLLQMEGYKAIGAANALEALAVANHM
                     DPDLILLDVMLPPMDGLTFLMRLREDERRRELPVIVVSGLSDPQTIARAKELGVKEHL
                     VKTHFTPQELLDAVRRYVQPGNVDAKRT"
     gene            complement(4224..4637)
                     /locus_tag="VLI90_06955"
     CDS             complement(4224..4637)
                     /locus_tag="VLI90_06955"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSV13982.1"
                     /translation="MVATLKMSQQHDETHRYQEQQQATPQAYMRLVPAPQGAERRMFE
                     RKEVHAHIQGKRLDHSIAARREPFLSLATRDLSIGGLSAISQTIIQRGERVSVYFPPQ
                     GVHRGWDALGRVIRCEPSGMGYRVAVEFEQMPLAA"
     gene            4775..>5407
                     /gene="cobA"
                     /locus_tag="VLI90_06960"
     CDS             4775..>5407
                     /gene="cobA"
                     /locus_tag="VLI90_06960"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR01469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HSV13983.1"
                     /translation="MNPGIVYLVGAGSGDPGLITVRGAELLATADVVVYDALANPRLI
                     QLHAPRAETIYVGKRAAAHSMTQDQINAVLVEQGRLGKRVVRLKGGDPFVFGRGGEEC
                     EALATAGITFEIVPGITAAIAAPAYAGIPVTHRGVATSVTFVTGHEDPSKPETQTDWA
                     ALARAGGTIVLYMGVKTLPRIAAALAAGGMSPETPAAAIQWGTHARQRTVS"
BASE COUNT         1037 a         1628 c         1764 g          978 t
ORIGIN      
        1 tctgggcggg gaggaatcat cgggcgacca ggctgcatca tgcttcccgc acgcggtcgc
       61 cggccattag aagatcgagc gatccttctc atgcgttgca agatttgctc gggtatcttc
      121 gctcgccctc gtatcattga attctcatct tttgccggtt tcacgctcct gtcagaagtc
      181 cggagcgatg agtcgcgcac tgtttgataa cgttgccgcg tcgcgcttct ctttgtggtg
      241 caaagctgct aaatattgaa gccagtggat ggagcggcgg cccgaggttc cgatgcctcg
      301 gctctcacgc aagtacggtt ggatttgtgc aagccagacc gcgcggtcag gacctcagcg
      361 caaaacccgg atgccctctt cgttcttcga gattctcgaa tcgcgcctct gtctgtcggc
      421 ggcgccggcg gcggggaact gggatctgta cgcgtcgttt gatttcaaca cgccgccgac
      481 cgcgcccact ttcgtcaata cgctttgggg gcacacgcat ttcccgggtg agcttgagag
      541 ctatcagccg gcaaatgtca gcgcatccaa tggcgttgcc tcattgactg cgcggcaaga
      601 gagcgtgggc ggaatgccgt tcagcagcgg catcatcaac accggtggga acaccgcgac
      661 gggcggcagc atctcgccgg ggttttcgtt tttgtatggg tacatcgaag ggcggatgaa
      721 gctcgtcagc ggcaacgggt tctggacggg tttttggatg ctgcccacgc cgaacgccaa
      781 cgggtatcac gatggcgatg gcgagatcga catcatggag cagatcggct cggatccgac
      841 cagcatcgcc ggcagcttgc atcacgatcc gaccaaccgc acgaaggatt ttcacagcgg
      901 cgtggatctg tcggctgact ttcacaccta cggcgtcgat tgggagcccg accacatcac
      961 gtggtacgtg gatcgttcgc cctacctgac gatgacgacg gatgtgccga acgtgccgat
     1021 gtacgtgatc ctgaacctgg cgatcggcga cgcgacgtcg tgggcgggca cgccggatgc
     1081 gacgacgccg tttccgacga cgatgcaggt ggattacgtg cacgtgtaca agtcggtgcc
     1141 gccggcgatc gcgggggacg cgaacatcga cggccgggtg gacgtggctg acttgggcat
     1201 tgtcgcgacg aacttcggcg tgaccagcgg ggcgacctgg gcgaccggcg atttcaccgc
     1261 ggatggtcgc gttgacgtgg ccgatctcgg gcagttcgcg accaactacg gctacggagc
     1321 acagacattc gcggcttcgc aaatcacatc acagcagatc gtcaagccgc aagcggcgga
     1381 cgcgctggat cctgatccgt tgcgcgagta cacaaaacct cgcctggcgg tcataatcct
     1441 gccgtgaaag aacgcattca gaaagtcctc ggcaacgcgg gggtggcgtc gcggcggaat
     1501 atcgaggaga tgatccgcca gggccgaatc agcgtgaacg gccgggtggc cacgcgattg
     1561 cctgtcttga tcgatcccgc gcgtgatcgc gtgaccgtcg acgatgaacc aatcaagcta
     1621 gctccggcca aagatggtgg ccccggaaag gccgtcggcc ggcggtttta cttcctgctc
     1681 aacaagccca gaggcgtcta cagcaccaac gtcgcgcagg gcgcgcaggt gcgggcgatc
     1741 gatttgctgc ccaagtcgtt cggcgggcgc gtgtatcccg tcggccgact ggatgctgag
     1801 agccgcgggc tgctgctgct gaccaacgac ggcgagctga cgaaccagct gacgcacccg
     1861 cgctatggcg tgtcgaagac ctatcgcgcg gtcgtcgacg gacacgtcgc gccggaagat
     1921 ttggtgaagc tcgagaaggg catttggctg gcggacccgc acagcgggaa aggtttcaag
     1981 accggtcgca gtcgaatccg catcgtcaaa cgcgcgcgcg acaagagcat cctggaaatc
     2041 acagttcgcg aaggccgcaa ccggcaggtg cggcgaatgc tggcgaatgt ggggcacaag
     2101 gtgcgcgagt tgacgcggat caagatgggg ccgctgacgc tggatcgact gcagccgggt
     2161 gcgtttcgcg agctgacggc gcgcgaggtg aaggcgctgc ggcagttggc ggaacggcac
     2221 gagcaaacca tcgcatccga ggcgggggat gatgttgggg aagaatgatg tggccgacgt
     2281 ttgtggcttt gctggtcgag gcacttggtt tgcgaagccg cgagcggcgg gatacgttcc
     2341 ggaacgatgc cgatttccaa tccaatgaac gtcgtcgatc gcctccgccg cttccagcgg
     2401 ggcgtgcgcg acatgatcat ccgctcgcgc aaaagcagcg gctcgatgta cgaagtcagc
     2461 cattccaccg tggccgacac gatctacaag atcgacacgc aggtcgatcc gctgctcgaa
     2521 gggttttgcc aggagtgggg ccgggaaacg ccgctggtgt tgatcgccga ggggatcgag
     2581 gacgagcacg gcgaggaggc ggcggtgacg tttccgcgcg gcacgcgcga ggccgacgcc
     2641 gagatacgcg tgatcatcga tccgatcgat ggcacccgcg gcatcatgta cgacaagcgg
     2701 gccgcatggg cgctggcggg cgtggcgccc aataaggggc ctggtacgcg gctgcgcgat
     2761 attgaaattg cggtgatgac cgaattgccc acgagcaaga tgggcttcgc cgacgtgctg
     2821 tgggcgatca agggccgcgg cgcgcacggt gtgcgcgagt cgctcaaccc cagcgaggtt
     2881 ccgcttgaag acgccgtgcc gatcgggcgc gtcctgacga agatgggcaa ggtcgccacc
     2941 gagcaggtgg ccgaggcgct gcacatgcag cggaccaaag gtgggctggt cggccagatt
     3001 cttgttggca tgggtgtcgt caacgaaagc gacgtgcaga tcgcgctggc gtttcaaaag
     3061 ggacagaagc cggacgtcgg tggtgaggtt gccaccgcca tcgccggcga tgcgctttcg
     3121 cttcattcca gccaggccgt cacgatcaac cacggcttcg ccagcgtcag caactttttc
     3181 cccgggacca aggtgctggc cagcgaattg atggagcacc tggtccgcaa cctgatcggc
     3241 gaagccgacg tgaccaaggc aaccgtcttt gacgatcagt acatctgcac gggcgggcag
     3301 ttttacgaac tgatgatcgg ccacgaccgc ttcaacgccg acctgcgtcc caccttctat
     3361 cggatgcagg gccagcccga aggactgtgc tgccatccat atgactgcgc gacgatgctc
     3421 atcgccgaag aagccggcgt gatcctgacg gatgaacgcg gcgatccgct ggatggtccg
     3481 ctcgacacga ccaccggcct gtcatggacc ggctacgcca acaccgaact gcggcgaaaa
     3541 gtcgaaccgc tggtgttgga attcttggcg cgggcaagca agaaaacctg aacgacggag
     3601 acgccatgtc gcggagaggt gccgtacatt gaacctgcct ccgcgccgct gcatctctgc
     3661 gattcacgat tgatcccgac agggccgctc cgatttctcc cattccgatt tgcctccgcg
     3721 cgtgggcata ctatatcgat catatgagta cgatcctggt cgttgatgat cacgccgata
     3781 cccgcaagcc gctcgttcgt ttgctgcaaa tggaaggata caaggccatc ggcgccgcca
     3841 atgcactgga agcgttggcg gtggcgaacc acatggatcc cgatctgatc ctgctcgacg
     3901 tgatgctgcc gccgatggat ggtttgacgt tcctgatgcg cctgcgcgaa gacgaacgcc
     3961 ggcgcgagct gccggtgatc gtcgtcagcg gcctatccga tccgcagacg atcgcccggg
     4021 ccaaagagct gggcgtgaaa gagcatctgg tgaagacgca cttcacgccg caggaattgc
     4081 tggatgccgt gcggcgatat gtgcaaccgg ggaacgtgga cgcgaagcgg acgtgagctt
     4141 tgtattgaat gcagaatgca gaatgaagac aggtgacccg tcttcattct gcattcaaca
     4201 ttcattgttc tgcattttca ccatcacgcc gccagcggca tctgctcgaa ctctaccgct
     4261 acccgataac ccatcccgct gggttcgcag cgtattaccc ggccgagggc gtcccaccca
     4321 cgatggacgc cctgcggcgg gaaataaaca ctcactcgct cgccccgttg aatgatcgtc
     4381 tggctgatcg cggacaaccc gccgatcgac aggtcgcgcg tcgccaggct caggaacggt
     4441 tcacgccggg cggcgatcga atgatccaac cgcttgccct ggatgtgggc gtgcacttcc
     4501 ttgcgctcaa acatgcggcg ttcggcgccc tgcggcgcgg gcaccagccg catatacgcc
     4561 tgcggcgtcg cctgctgttg ctcctgatac cgatgcgttt cgtcgtgttg ctgcgacatc
     4621 ttcaacgtgg caaccattgg cgtctccctg cgacctgcct gtagaatcgg tccccggaca
     4681 ctgaactcat cggcgcagaa gcagggcggg tttatcaccc ttgcccagac ggaatagaca
     4741 gacattggct actgtaacct ccaataaaaa cggcgtgaat cccgggatcg tttacttggt
     4801 cggcgcgggc tcgggtgatc ccggtctgat caccgtgcgc ggggccgaat tattggccac
     4861 cgctgacgtg gtcgtgtacg acgccctggc caacccgcgg ttgatccagc tgcacgcgcc
     4921 gcgcgcggag acaatctatg tgggcaagcg cgccgccgcc cattcgatga cgcaggacca
     4981 gatcaacgca gtcctggtgg agcaggggag attgggcaaa cgggtcgtgc ggctcaaggg
     5041 cggcgatccg ttcgtcttcg gccgcggcgg cgaagaatgc gaagcgctcg ccaccgcggg
     5101 gatcacattc gaaatcgtgc cgggcatcac cgccgccatc gcggcgccgg cgtacgcggg
     5161 gattcccgtc acgcatcgcg gcgtcgccac atcggtcacg ttcgtcacgg ggcacgagga
     5221 tccatccaag cccgagacgc agaccgactg ggctgcgctc gcgcgtgccg gcggcacgat
     5281 cgtgctgtac atgggcgtga agacgctgcc gcgcatcgcc gccgcgctcg cagcgggcgg
     5341 catgtcgccg gagacaccgg ccgcggcgat acagtggggc acgcacgcca ggcaacgcac
     5401 cgtgagc
//
LOCUS       DATIMH010000403         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3846543, whole genome shotgun sequence.
ACCESSION   DATIMH010000403 DATIMH010000000
VERSION     DATIMH010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3846543"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VLI90_06965"
     CDS             complement(<1..519)
                     /locus_tag="VLI90_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSV13984.1"
                     /translation="MTAVATRIPVSTRFDVEGVRADFPILHAQFNGHPLVYLDNGATT
                     QKPRTVIDALSRYYESQNANIHRGVYHLSQLATQLYDESRERIARFINANESKEVIFT
                     RGTTESINLVAGCWGRTFLKSGDEVIVSAMEHHSNIVPWQIACEMTGAKLRVIPMNDA
                     GELQLDEYAKLLS"
     gene            complement(516..1118)
                     /locus_tag="VLI90_06970"
     CDS             complement(516..1118)
                     /locus_tag="VLI90_06970"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV13985.1"
                     /translation="MTLLASKPTTRTYTPEELLSLPNGNSFELVGGHLVEKNVSLESS
                     NVEALFAFRFQAYAMERGIVAVFPSSLGYRCFRDEPDKVRRPDVTVVRVERIAALPEP
                     NPGFMPIVPDLAVEVVSPNDIVYEVEEKVQEYLAAGFPLVWVAHPKTRTVTVHPLSDR
                     PAIFTADDELTAESVMPGFRCKVADFFSAPAATPADGMRT"
     gene            complement(1150..>2405)
                     /gene="sufD"
                     /locus_tag="VLI90_06975"
     CDS             complement(1150..>2405)
                     /gene="sufD"
                     /locus_tag="VLI90_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HSV13986.1"
                     /translation="NETGYPGRRDEEWRFTNVDPIAKTKFALAPRIEDASSIEAATQF
                     SFGRDAVSELVFINGHYTPQLSKLGKLPRGVKVQSLAEALSANPAELKSHLSKHADIN
                     TNPFVALNTGHIHDGAYIHFPRGATVEGAIHLLFVSVPTPQPTVTHPRILVIAEENVE
                     ATLVESYVGEGSGGYFTNTVTEIIAASDSRIDHCKLQQESHEAYHLSAMQVHLGRSAH
                     FVTHGATIGGKLSRNEINVTLAGEYADATLNGLVLLDGARHCDNHTLLDHAAANCPSH
                     ELFKHVLGGKSTGVFKGKILVRPDSQKTDSKQTSKTLLLSDDAIMDSMPALEIYADDV
                     KCTHGSTTGPVDEEQVFYLRSRGVSHEAARHLLTYAFAADITRRIRVEPVRARIENFM
                     AAQHGLPLDLRIADLGAHDEAVVNI"
BASE COUNT          397 a          713 c          786 g          509 t
ORIGIN      
        1 gctcagcagc ttcgcatact cgtccagctg aagttcgccg gcgtcgttca tcggaatgac
       61 gcggagcttc gcgccggtca tctcgcaggc gatctgccac ggcacgatgt tggagtgatg
      121 ctccatcgcc gagacgatca cctcgtcgcc gctcttgaga aacgtgcggc cccagcaccc
      181 ggcgaccagg ttgatgctct cagtcgtccc gcgggtgaag atgacttcct tcgactcatt
      241 ggcgttgatg aagcgggcga ttcgctcgcg cgattcatcg tacagctgcg tcgccagctg
      301 gctcaggtga tacacgccgc ggtggatgtt ggcgttttgg gattcgtaat agcggctgag
      361 cgcgtcgatg acggtgcgcg gcttctgcgt cgtcgcgccg ttgtcgaggt agacgagcgg
      421 atggccgttg aattgcgcgt gcaggattgg gaagtcggcg cggactcctt cgacatcgaa
      481 gcgtgtggag acgggaattc gagtcgcaac ggcagtcatg tccgcattcc atccgctgga
      541 gtggctgccg gcgcggagaa gaaatcggcg accttgcagc ggaatcctgg catcaccgat
      601 tcggcggtga gttcatcatc cgcggtgaag atcgccggcc gatcgctgag cggatgcacc
      661 gtcaccgtcc tcgtcttcgg atgcgcaacc cagaccaatg ggaagcctgc cgccaaatat
      721 tcctgaacct tctcttcaac ttcgtacacg atgtcgttgg gcgagacgac ttcgaccgca
      781 aggtccggca cgatcggcat gaagcccgga ttcggttcgg gcagcgccgc gatgcgttcg
      841 acgcgaacga ccgtcacatc aggccttcgc accttgtccg gctcgtcgcg aaagcaacgg
      901 tagccgaggc tagatgggaa aactgctacg atcccgcgct ccattgcata cgcctgaaac
      961 cggaatgcga agagggcttc gacgttactc gattccaggc tcacgttctt ctccaccaga
     1021 tgccccccga ccagctcgaa ggagttgcca ttcggcagcg atagcaattc ctccggcgtg
     1081 tacgttcgag tggtcggttt tgaggcgagt agcgtcacga gcgactcctg tgcaacttgt
     1141 cactgcattc tagatgttca cgaccgcttc gtcgtgggcg ccgagatccg cgatgcgcag
     1201 atccaacggc agcccatgct gagcggccat gaagttttcg atccgtgccc gtaccggttc
     1261 gacgcggatt cgccgtgtga tgtcggcggc gaaggcgtat gtgagcaggt gccgcgccgc
     1321 ttcgtggctc acgccgcggc tgcgcagata aaagacctgc tcttcatcga ccggccccgt
     1381 ggttgagcca tgcgtgcact tcacgtcgtc ggcgtagatc tccagcgccg gcatcgaatc
     1441 catgatcgcg tcgtcgctca gcagcagcgt cttgctcgtc tgttttgaat cggtcttctg
     1501 gctgtccggg cggacgagga tctttccctt gaacacgccc gtggacttgc cgcccagcac
     1561 gtgcttgaac agttcgtggc tggggcagtt cgccgcggcg tgatcgagca gcgtgtggtt
     1621 gtcgcaatgc cgcgcgccgt ccagcagcac caggccgttc aatgtcgcgt cggcgtattc
     1681 accggcgagc gtgacgttga tttcgttgcg gctcagtttt ccgccgatcg tcgcgccgtg
     1741 cgtgacgaag tgggcgctgc ggcccaggtg cacctgcatg gccgacaggt gatacgcctc
     1801 gtgcgattcc tgctgcagct tgcagtggtc gatccggctg tcggaggcgg cgatgatttc
     1861 ggtgacggtg ttggtgaaat atccgccgct gccttcgccg acgtagcttt ccaccagcgt
     1921 cgcttcgacg ttctcctcgg cgatcaccag gatgcgcgga tgggtgacgg tcggctgagg
     1981 cgtcggcacc gaaacgaaca gcaggtggat tgcgccctcg acagtcgcgc cgcgcggaaa
     2041 gtggatgtag gcgccgtcgt gaatgtggcc ggtgttaagg gcgacgaaag gattcgtgtt
     2101 gatgtcggcg tgcttcgaca ggtgactctt caattcggct gggttggcgc tgagcgcctc
     2161 ggccagcgac tgcaccttca cgccgcgcgg cagcttgccg agcttggaca gttgcggcgt
     2221 gtagtggccg ttgatgaaga ccagttcgct gacggcgtcg cggccgaagc tgaactgcgt
     2281 ggcggcctcg atgctgctgg cgtcttcgat ccgcggggcg agcgcgaact tggtcttggc
     2341 gatcggatcg acgttggtga agcgccactc ttcgtcgcgc cggccggggt aaccggtttc
     2401 gttga
//
LOCUS       DATIMH010000404         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4136355, whole genome shotgun sequence.
ACCESSION   DATIMH010000404 DATIMH010000000
VERSION     DATIMH010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4136355"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="VLI90_06980"
     CDS             <1..1750
                     /locus_tag="VLI90_06980"
                     /inference="COORDINATES: protein motif:HMM:NF038122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NF038122 family metalloprotease"
                     /protein_id="HSV13987.1"
                     /translation="PPIIHPAPAPVPPAPFTPPVVPASPTAFTISIRWDSSVAAAPAG
                     FATDIVAAVQYLETQFSDPITMTINVGYNEVGGSPLSGGALGESLTNLSPISYATLRN
                     AVASDARTATDAAVLASLPATSAIDGATYWVADAQAKALGMTVSGGGADGSVGFGAGS
                     LFTFGDTANGGTVAAGTYDFFAAVVHEITEVMGRQMLTGATFGGSRTSETLLDLLHYA
                     APATRDLSARTAGYFSPDGGVSNLGAFNTASGGDAGDWASSASPDSFDAFATSGGIEG
                     VTANDLTVLDAIGWDAAGAGGTTPPVNPPVTPPVTPPAVTPTGLDVTAETRFLAHAQG
                     RSGLSAVSPLAMVTQSGGTPGDAFSYALGGAGAASFGLIDAGSGAILTTGWRGVAGAT
                     GGKLYALTVTATDQTANGNPFVTGAINVLVGENANDAMNLASITGVVAAAPTFIYGLG
                     GNDTIDGSGMTGALYFDGGAGADTMTGGSGVNTYEYGGAVDSSATAMDIITNFHPGVD
                     LIDLSGLGRELGAATAIDPSATTIGAGTIGWQTSGGNTFVYVNTAFRTETLGSANMKI
                     ELAGTVALTGDSFVHL"
     gene            2130..>2436
                     /locus_tag="VLI90_06985"
     CDS             2130..>2436
                     /locus_tag="VLI90_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13988.1"
                     /translation="MRNLDLNKGLFSGDEDSTIWDTGDSANAAPGESKSTPASDTPSG
                     PATATQTDFAVGGSSQTGTAVATGASLPVVPPSPSAFTIAVNWDASVSSAPAGFTSDI
                     "
BASE COUNT          430 a          775 c          814 g          417 t
ORIGIN      
        1 gccgccaatc attcaccccg cgcccgcccc ggtgccgccg gccccgttca cgccgccggt
       61 ggttccggca tcgcccaccg ccttcaccat cagcattcgc tgggacagca gcgtcgccgc
      121 cgcgcccgcg ggcttcgcga ccgatatcgt cgccgcggtt cagtacctgg aaactcagtt
      181 ttccgacccg atcacgatga ccatcaatgt cgggtacaac gaggttggcg gcagtcctct
      241 cagcggcggc gctctgggcg agagcctcac caatctctcg ccgatcagct acgccacatt
      301 gcgcaatgcg gtcgcatcgg acgcgaggac cgcgaccgat gcggccgtgc ttgcgtcgtt
      361 gccggcgacg agcgcgatcg acggcgcgac ttactgggtg gccgacgcgc aggcgaaagc
      421 tctgggcatg accgtcagcg gcggcggcgc ggacggcagt gtgggttttg gggcgggatc
      481 gctgttcacc ttcggcgata ccgccaacgg cgggacggtc gcggctggga cgtacgactt
      541 cttcgccgcg gtggtgcatg aaatcaccga ggtgatgggt cgccagatgc tgaccggtgc
      601 gacgttcggc ggttcccgga cgagtgaaac gctgttggac ctgctgcatt acgccgcgcc
      661 cgcgacccgg gatttgtcag ccaggacggc gggatatttc tcgccggacg gcggtgtcag
      721 caacctcggc gccttcaaca cggcatcggg aggcgacgcc ggcgactggg cgtcctccgc
      781 gagccccgac tcattcgatg cgtttgccac ctccggcggg attgaggggg tgacagcgaa
      841 cgacctgacg gtcctcgatg ccatcggctg ggatgcggcg ggtgccggag ggacaacccc
      901 gccggtgaac ccgcctgtta cccctccggt cacacctccg gcggtgacgc cgacgggcct
      961 cgacgtcacc gccgagacgc gctttctggc gcacgcccag ggacgatcgg ggttgtcggc
     1021 ggtgagtcca ttggcgatgg taacgcaatc cggcggcacg ccgggcgatg catttagcta
     1081 tgctttgggt ggggcgggcg cggcatcctt cggcctgatc gatgcgggta gcggcgcaat
     1141 tttaaccacg ggctggcgtg gcgtggcggg cgcgaccggc ggcaagctgt atgcgctgac
     1201 cgtgacagcc accgatcaga cggcgaacgg caatcccttc gtcaccggtg cgataaacgt
     1261 gcttgtcggt gagaatgcca atgacgcgat gaacctcgct tcgatcaccg gagtcgtggc
     1321 cgcggcgccg accttcatct acggactggg cggcaacgac acgatcgacg gctcggggat
     1381 gaccggtgcg ctctatttcg acggaggggc cggggccgat accatgaccg gcggcagcgg
     1441 tgtgaacacc tacgaatatg gtggcgccgt cgattcgagc gccacggcga tggacatcat
     1501 caccaatttc catcccggag tggatttgat cgatctgagc ggcctcggcc gggagctcgg
     1561 cgcggcgacg gcgatcgatc catcggccac gacgatcggc gccggtacca tcggttggca
     1621 gacgagcggc ggcaacacgt tcgtttacgt caacacggcc ttccggacgg agacgctggg
     1681 gtcagccaac atgaaaatcg agttggcagg caccgttgcc cttactggcg acagtttcgt
     1741 ccacctctga cggacccgtg acgggccatc cagggacagg cgcgaccctt tagcgccgaa
     1801 gggccatacc gaacgtcagt gttgccgggc ggcgggcgag aacccccgag cgcttcgggg
     1861 gcgccgcata gggaacctgg attactccgc ggcaatcgtc tccgtctgga agccagccgg
     1921 ctgtaagccg ggcggataca tctataattt gggaaatata tcgtgtatct gtcttgacga
     1981 tgatcaaagc gaaacgttat tgacacccat cgctcatcaa tttgcctgag caatccgagc
     2041 cttccctctt atcggaaaaa atgcgcggtt gtggtgttcg ggccgagggg gccagggctg
     2101 agggtagatt aacgtcgaca ggatctcaca tgcgaaacct cgatttgaac aagggcctgt
     2161 tttcaggcga tgaagactcg accatctggg ataccggcga cagcgcgaac gcggcgcccg
     2221 gcgagagcaa gtcaacgccg gcgagcgaca caccatcagg cccggcaacc gccactcaga
     2281 ccgacttcgc ggtcgggggc tcttcgcaaa cgggcacggc ggtcgccacg ggcgccagcc
     2341 tcccggtcgt gccaccgtca ccatccgcgt tcacgatcgc ggtgaactgg gacgcgagcg
     2401 tcagttcggc gccggccggc ttcacgagcg atattc
//
LOCUS       DATIMH010000405         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5006207, whole genome shotgun sequence.
ACCESSION   DATIMH010000405 DATIMH010000000
VERSION     DATIMH010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5006207"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="VLI90_06990"
     CDS             <1..1725
                     /locus_tag="VLI90_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13989.1"
                     /translation="ITTTAHADGDLSNVLGVAHADGKYFLTDGDYLNEGADQVAASGS
                     KVLKLWFNPVRYPWNSHWPDKVPAMVDLAQTPYYRAVFAKPFSTIILVAYSFGRDEHY
                     FVNGVTDDQLADETRQFHDLTAYLLTTYRGSGKTFVLQHWEGDWALRDDHTGHIYDDK
                     VTPTQKAIDSMIRWINARQAGISQARAEVKDSDVHVYGACEANRVEDSMAGRPGVANS
                     VLPHTTVDLASYSSYVSLTSPDRLSKAVDYLAGQLPPTAAFGQNPHSIYLGEFGYPEN
                     GDLGAAGVNDRINTVVQVVKAKQLPYAILWQTYCNEIKKDVTAAPPLNGPGNNDKFKG
                     YWLVKPDGKPSVAWHRYRQLIITSDDSRATASAIKAKLSPVFHDGFARPDGADIGPGW
                     TQSAHYGPIGGHLANHQLQLDIADGTTIQWTSDTLDLTNDKILGRGMNVGEYFEVKMQ
                     RHANRGMCGVELFDSDQLRQGSAMPDTPAPLHAWDGTTWTPLSIDAKGQPVAIDWNQP
                     HTLGVRFDSADGHFDTFSYYIDNTYAGSWLVKTGNRTLDKIGVFAQSDLSGASFTFAG
                     LTVYGRGK"
     gene            2043..2579
                     /locus_tag="VLI90_06995"
     CDS             2043..2579
                     /locus_tag="VLI90_06995"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HSV13990.1"
                     /translation="MAQVAPPRAPTTAPSPAAELTIFSEGTYPQPDVKRTTIYYCRPA
                     IRSPKGEDRKIWGGLVPWDKVWRLGANQATLLISQSTLQFGDDVTIPANTPVTLYMLP
                     VENGVSKLIINKRIGQSGVDYDEKQDLGRVDLKKDDLTQTVDRLILAMPMNKPGGGGT
                     LQIAWENTMFSVPFTVKK"
     gene            2834..>3548
                     /locus_tag="VLI90_07000"
     CDS             2834..>3548
                     /locus_tag="VLI90_07000"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HSV13991.1"
                     /translation="MVLVSKSARHSRRIAHLNRDRDRNRLRSSVDYVLERMESRTLLA
                     ATPIVSGQTIAGSLTHAGQQDTYTFTATAGKTFDVSVGDASAASGVHPYLQVFAPGGA
                     RVINNATSATSTSVDGVITVPSTGAGTYTIDVQDFLGNGTGAYDVELAVAPSTQATDS
                     NGDGGVIKSGQTKAGTINRFGDIDVFTFSASAGNTFDISLGDATSGSSVRPYLQVFNP
                     SGTRVINNATSATATSVDGI"
BASE COUNT          717 a         1195 c         1004 g          632 t
ORIGIN      
        1 attacaacca ccgcccacgc cgacggcgat ctctccaacg tcctcggtgt cgctcacgcg
       61 gacggcaaat atttcctcac cgacggcgac tacttgaacg aaggcgccga ccaggtcgcc
      121 gccagcggct ccaaggtcct caagctctgg ttcaaccccg tgcgctatcc gtggaacagc
      181 cattggccgg acaaggtgcc ggcgatggtg gacctggcgc agacccccta ctaccgcgcc
      241 gtcttcgcca agcccttcag cacgatcatc ctcgtcgcct actcgttcgg ccgcgatgaa
      301 cattacttcg tcaacggcgt cacggacgat caattggccg acgaaacccg gcagttccac
      361 gacctcaccg cgtatcttct gacgacctac cgcggcagcg gcaagacgtt tgtcctgcaa
      421 cattgggaag gcgactgggc cctgcgcgac gatcacacgg gccatatcta cgacgacaaa
      481 gtcacgccaa ctcaaaaggc gatcgacagc atgatccgct ggatcaacgc gcggcaagcc
      541 ggcatcagtc aggcgcgcgc cgaggtgaag gacagcgacg tgcacgtcta cggcgcctgc
      601 gaagccaacc gcgtggaaga ctcgatggcc ggccggcccg gcgtcgccaa ctcggtgctg
      661 ccgcacacca ccgtcgacct cgcgtcttac tcgtcgtacg tctcgctgac gtcgcccgat
      721 cgactgagca aagccgtcga ctatctcgcc ggccaattgc cgccaacggc cgccttcggg
      781 cagaacccgc acagcattta cctcggcgaa ttcggctatc ccgagaacgg cgacctcggc
      841 gccgccggcg tcaacgaccg catcaacacc gtcgtccaag tcgtcaaagc caagcagctg
      901 ccctacgcca tcctctggca gacttactgc aacgaaatca aaaaagacgt caccgctgcg
      961 ccgccgctga acgggcccgg taacaacgac aagttcaagg gctattggct ggtcaagccc
     1021 gacggcaagc cgtcggtcgc gtggcatcgc tatcgccagc tgatcatcac ctcggacgat
     1081 tcacgcgcga ctgcatctgc gatcaaagcg aaattgtcgc cggtcttcca cgacggcttc
     1141 gcccgccccg acggcgccga catcggcccc ggttggacgc agtccgccca ctacggcccc
     1201 atcggtggtc acctggccaa ccatcaactg cagctcgaca tcgccgacgg cacgaccatc
     1261 cagtggacca gcgacacgct cgatctgacc aatgacaaaa tcctcggccg cggcatgaac
     1321 gtcggcgaat acttcgaagt gaagatgcag cgccacgcca accgcggcat gtgcggcgtc
     1381 gaactgttcg actccgacca actccgccaa ggctccgcca tgcccgacac ccccgccccc
     1441 ctgcacgcct gggacggcac cacctggacg cccctctcca tcgacgccaa aggccaaccg
     1501 gtcgcgattg attggaacca accccacacg ctcggcgtcc gcttcgactc cgccgatggc
     1561 cacttcgaca cgttcagcta ctacatcgac aacacctacg ccggctcctg gctggtgaaa
     1621 accggcaacc gcacgctcga caagatcggc gtgttcgccc agtccgatct tagcggcgcg
     1681 agcttcacgt tcgcggggct gacggtgtac ggccggggga agtgagtgcg tgagcgggtt
     1741 agcgggttag cgggttaggg agttggggat aatcagaaaa agattcatac cactgccgcc
     1801 gtcttcatct gcgatggtca gcgtgaactt ggttgaccac ctggctcgga atcgtgccac
     1861 gccatctggg gcgcttgttc aagtgctaag aggttgcgag aatagtcgtc cactcaggag
     1921 gaatcatgag cagctcaatc gtggtccgcg gcaccgttcc atctcgactg gcgcgacgac
     1981 atatttcgag tttgttcgtt ctgatcacga tctgcttcac cctcgcgccg cgggcgctgt
     2041 tgatggctca ggtcgcgccg ccgcgtgcgc cgaccaccgc gccaagcccg gcggcggagc
     2101 tgactatctt ctcggaagga acttatccgc agccggatgt gaagcgcacg acgatttact
     2161 actgccggcc ggccatcagg agcccgaagg gcgaagatcg caagatctgg ggcggcctcg
     2221 tgccatggga caaagtctgg cggctcggcg ccaatcaggc gacgctgctg atctcgcaat
     2281 ccacgctgca gttcggcgac gacgttacca tccccgccaa caccccagtt acgctctaca
     2341 tgctgccggt cgaaaacggc gtgtccaagc tgatcatcaa caaacggatc ggccagtccg
     2401 gcgttgacta tgacgagaag caggacctcg gccgggtcga cctgaaaaag gacgacctga
     2461 cccaaacggt cgaccgcctc atcctcgcca tgcccatgaa caaacccggt ggcgggggaa
     2521 cgctgcagat cgcgtgggaa aacacgatgt tttctgttcc atttacggtc aagaaatgac
     2581 gccgcacggc tacatcacct tgacatgacc cgcggcatgg cgtcccggcg atcatagaaa
     2641 ctgccgatcg tggtctaggt atctttgcct gcgtcggtgt gcgcctctgc gttcttccaa
     2701 agaaccttag tcattatttc ttgcgttcaa ccggaatgag cattacttat ctatgttggg
     2761 aagttgattc ctccggctag ggaatctcat ctcttcttca tgtcgaactg cacaaatggg
     2821 caggaggtcc actatggttc tcgtctcgaa atccgcgcgt cactctcgtc gcatcgccca
     2881 cctcaaccgc gatcgcgatc gcaatcggct gcgcagcagc gtggattacg tgttggagcg
     2941 aatggaatcg cgcacgttgc tggcggcgac gccgatcgtt tcggggcaga cgatcgccgg
     3001 gagcctcacg catgcggggc agcaggacac gtacaccttc accgccacgg cgggcaagac
     3061 gtttgacgtt tccgtcggtg acgcctcagc cgccagcggt gtgcacccct atctccaggt
     3121 gttcgcgccc ggcggggcgc gggtgatcaa caatgccacg tcggcgacgt ccacctccgt
     3181 ggacggcgtc atcaccgtcc catccaccgg cgcggggacc tataccatcg acgtgcagga
     3241 tttcctgggc aacggcaccg gcgcgtacga tgtggagctg gccgtcgctc cttcgactca
     3301 agccaccgac agcaacggtg acggtggcgt catcaagagc gggcaaacca aggccggcac
     3361 catcaaccgc ttcggtgata tcgacgtctt cacgttctcc gcctcggcgg ggaacacgtt
     3421 cgacatctcg ttgggcgacg cgacgtccgg cagcagcgtg cggccgtatc ttcaggtctt
     3481 caacccgtcg ggcacgcgcg tcatcaataa tgctaccagc gcgaccgcta catccgtcga
     3541 tgggatct
//
LOCUS       DATIMH010000406         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_385130, whole genome shotgun sequence.
ACCESSION   DATIMH010000406 DATIMH010000000
VERSION     DATIMH010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385130"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1917
                     /locus_tag="VLI90_07005"
     CDS             <1..>1917
                     /locus_tag="VLI90_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HSV13992.1"
                     /translation="GTITSGVGLISGINTSSIIDQLMALESKPKDLLQTQIDSTNQQK
                     LAYTDLVARLTSLQITGQTLEKPSNFQAAKTTSSDENVLTATASNGAAIGSYSLQVAR
                     LTTTQQMVSDGFTDFNSTPIGAGTITIEQGGGEVSSQTTLAQLNGGAGVNRGQFRITD
                     RSGASSVIDTSADVTVDDVLRKINTSLDVSVRATLQGDKIVLNDTSGKAANDLIVQDL
                     GSGTAAADLGIAGNVAGNALTGTDVNYVGRNTLLNQINDNRGIRRAASGNDFDVTVSD
                     GSTISVNLATAKSIGDVIDAINTAGGSKLKADIVPGSNGLRLTDTSGGGGSLSIAQTN
                     GSQAAQDLGLLANPTGSVINGGDIVAGIDTSLISSLRGGSGLSLGQVSFTDRAGHSRT
                     VDFAGASGVQDILDRINNAGGGLQLTASLKDSGNGIEITDTSGGSGNIVIADNGGGQT
                     ATQLGIAGTFNAATPAVLGANLQKQWITSNTLLSTLNGGKGISKSQFKITNSLGQVST
                     IDLSSANVTTVGDVLYRINNPPLNGVTASINANGDGILISDTSAGAGKLTIADLGGTA
                     AADLNIKGAATGTTIDGSYEKTITISANDTLATVQTAINNLNFGVTASVINDGSGTAP
                     YRLSLTAKNSGRSGR"
BASE COUNT          407 a          650 c          571 g          289 t
ORIGIN      
        1 gggcacgatc acctccggcg ttggactcat cagcggcatc aacaccagct ccatcattga
       61 tcagttgatg gccctggagt cgaagccgaa ggatctgctc cagacccaga tcgactcgac
      121 gaaccagcag aagctggcct atacggatct cgtcgctcgc ctgacttctc tgcagatcac
      181 cggccagacg ctggagaagc cttccaactt tcaggcggcc aagaccacca gcagcgatga
      241 gaacgtgctg accgccaccg cgagcaacgg ggctgcgatc ggcagctata gcctgcaggt
      301 cgcacgcctc accacaactc agcagatggt cagcgacggt ttcaccgact tcaattccac
      361 gccgatcggc gccggcacga tcacgatcga acaaggcggc ggtgaagtgt cgtcgcagac
      421 gacgctcgcg caattgaacg gcggcgccgg tgtgaaccgc gggcagttcc gcatcaccga
      481 tcgctctggc gcttcatcgg tcatcgacac cagcgcggac gtcacggtcg atgacgtgct
      541 gcggaagatc aatacctcgc tggacgtctc cgtccgcgca acgctgcagg gcgacaagat
      601 cgtcttgaac gacacctcgg gcaaagccgc caacgatttg attgtgcagg acctgggcag
      661 cggcacagcc gccgccgacc tgggcatcgc cgggaacgtc gcgggcaacg cgctgacggg
      721 gaccgacgtc aattacgttg gccgcaacac gctgctgaac cagatcaacg acaaccgcgg
      781 cattcgccgg gctgccagcg ggaacgattt cgacgtgacc gtctccgacg gcagcacgat
      841 cagcgtgaac ctggccacgg ccaagtcgat cggcgacgtg atcgacgcga tcaacaccgc
      901 cgggggttcg aagctgaagg ccgacatcgt ccccggcagc aacggcctac gcctgaccga
      961 caccagcggc ggcggaggct cgctcagcat cgcgcagacc aacggttcgc aggcggcaca
     1021 agaccttggc ttgctcgcga accccaccgg cagcgtgatc aacggcggcg acatcgtcgc
     1081 cggcatcgac acgtcgctca tctcctcgct ccgcggcggc agcgggttgt cgctggggca
     1141 ggtcagcttc accgatcgag ccggtcactc gcgcaccgtc gacttcgccg gcgcgagcgg
     1201 cgtgcaggac atcctcgacc gcatcaacaa tgccggtggt ggtcttcaac tcaccgcttc
     1261 gctgaaagat tccggcaacg gcatcgagat caccgacacc agcggcggca gcgggaacat
     1321 cgtcatcgcc gacaacggcg gtggacagac cgcgacgcag ctgggtatcg ccggcacgtt
     1381 caacgcggcc acccccgcgg tgctgggcgc gaacctgcag aagcagtgga tcaccagcaa
     1441 caccctgctg tcaacgctga atggcggcaa ggggatctcc aagtcacagt tcaagatcac
     1501 caattcactc gggcaggttt cgacgatcga tttgtcgtcc gccaatgtca ccacggttgg
     1561 cgatgtcctc taccgcatca acaatccacc gctgaacggc gtcaccgcgt ccatcaacgc
     1621 caacggcgac ggcatcctga tctccgacac cagcgctggc gcgggcaagc tcaccatcgc
     1681 agatctgggc ggcaccgcgg cggcagacct gaacatcaag ggcgctgcga ccggcacgac
     1741 gatcgacgga tcgtatgaga agacgatcac gattagcgcc aacgacacgc tggccacggt
     1801 gcagacagcg attaacaacc tgaactttgg cgtcaccgca tccgtgatca acgacggctc
     1861 gggcaccgcg ccatatcgct tgtcgcttac cgcgaaaaac tccggccggt ccgggcg
//
LOCUS       DATIMH010000407         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2115736, whole genome shotgun sequence.
ACCESSION   DATIMH010000407 DATIMH010000000
VERSION     DATIMH010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2115736"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VLI90_07010"
     CDS             complement(<1..408)
                     /locus_tag="VLI90_07010"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HSV13993.1"
                     /translation="MSNNLTGNAVIGQSGGPTSVINQSLAGVIQAAKKAGHIKELLGA
                     RHGVRGVINEDFIPLKTTPEDLLERIAQTPAAALGSTRDKPDQAYCEKIFASFKKNDV
                     RYFFYIGGNDSCDTARIVHDMAKAAKYDLKVFHI"
     gene            complement(486..911)
                     /locus_tag="VLI90_07015"
     CDS             complement(486..911)
                     /locus_tag="VLI90_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HSV13994.1"
                     /translation="MPYDEEDPPYANLVEPPAALDELARAVIGAAIEVHKELGPGLPE
                     EAYQRAMESELRLRGIPFERQKRIVVTYKGEPVALAKIDLVVGGMLIVELKAVEAIAK
                     IHVLQARTYMRILKQPLALLINFNVPVLKEGIRRVVETI"
     gene            complement(979..2091)
                     /locus_tag="VLI90_07020"
     CDS             complement(979..2091)
                     /locus_tag="VLI90_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV13995.1"
                     /translation="MAEKSVSTDSLAMETKQQYTGKKLRVAFIGCGGIAQTHLGALAT
                     MPDVEVVAGVDINPDRLDVMRDKWHVDKVYSDWKKMLKEVQPEAVSICTPNGVHAQPA
                     IDSSNAGAHVIVEKPMAMNPGQCQKMIDTAKKAGKKLAVGFQYRYHPNTEFLTRAREA
                     GEFGNVMFVKCQALRRRGIPNWGVFGRKDLQGGGPMIDIGVHVIEMAHYVMGSPKPVA
                     ASGNTWTYMGNKKSDVVSQWPNWDYKTYTVEDLAIGQIRFENGAILQIEASFAAHIEK
                     DVWNFTLVGDKGGASWDPPALFTDRAGTMINSTPGFLSSKTDFHSLFISKLRNFVDGC
                     MKDTALRAPGEAGLAVQKILDGVYRSADAGKEVKID"
     gene            complement(2227..>3597)
                     /locus_tag="VLI90_07025"
     CDS             complement(2227..>3597)
                     /locus_tag="VLI90_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13996.1"
                     /translation="LLVIAVAALACVLVCGHTPGVNGPWYWRWRWRRLDAVRFYPSML
                     AAAVPFFIGQFLWIKLAARRRPQFVRWILLLSMSSSLAMSFIATGLQMQPFGTAALYR
                     TMTDGNANGYLVDADVLMTTRERGQITLDGFLQQFPRIMPMLHIHSRIRPPGVILFFS
                     IFRGLFPDAHQAILVASLAIGILASLSIPATYLLLLELTGSISAGVAAASYLAMCPGF
                     VLFFPAVDTIYPLATVTLALLWLRALVRRRIGYAIAFGLVLAACCFCAFNVLVLGAFF
                     GGLTLLQIRRGARWGWAINCAVAGVVAFVLFWFALWLTTGFDIIATFKQALHQQALFE
                     QVARRPYPQSIPWDVYDFLLGSGWISAVLIAIGWRAWKRQGYTALVLLCLAQPLLVAL
                     LKLLQTETARVWLFMQPLLLVPVGLELAGWSPRQRIWVYAALVLITLTIGQNMWMVGW
                     TSAITP"
BASE COUNT          688 a         1069 c         1141 g          699 t
ORIGIN      
        1 gatgtggaag accttcaggt cgtacttcgc ggccttggcc atgtcgtgca cgatgcgggc
       61 ggtgtcgcag gagtcgttcc cgccgatgta gaagaagtag cggacgtcgt tcttcttgaa
      121 gctggcgaat atcttttcgc agtatgcctg atcgggcttg tcgcgcgtgg aaccgagggc
      181 ggcggcggga gtttgggcga tgcgttcgag caggtcctcg ggcgtagttt tgagcgggat
      241 gaagtcttcg ttgatgacgc cgcgaacacc gtggcgggcg ccgaggagtt ccttgatgtg
      301 cccggccttc ttggcggctt ggatgacacc ggcgagggat tggttgatga cggaggtggg
      361 accaccggat tggccgatga cggcgttgcc tgtgaggtta ttgctcatga tggttgttcc
      421 ttcgaagctt gtgcgtttga gtttgcgcca agagcgccaa gacgccaaga gcgccaaggg
      481 aagtatcaga tggtttcgac gacgcggcgg atgccttctt tcaaaacggg cacattgaag
      541 ttgatgagaa gcgcgagcgg ttgcttgagg attcgcatgt acgtccgcgc ctgcagcaca
      601 tgaatcttgg cgatcgcctc gaccgccttg agttcaacga tcagcatccc acctacaact
      661 aagtcaatct tcgccagtgc aaccggttca cctttgtagg tgacgacgat tctcttctgc
      721 cgctcgaatg ggatgcctct gagacgcagc tcgctttcca tcgcgcgctg atacgcctcc
      781 tccggcagtc caggtcccaa ttctttgtgg acctcgattg ccgcgccgat cacggcgcga
      841 gccagttcat ccaaggcagc aggtggctcc accagattgg catacggtgg gtcttcctca
      901 tcgtacggca tgagaccacc tgttctgtct tcccttggcg ctcttggcgt cttggcgctc
      961 ttggcgtaaa tctgaatctc aatcgatctt cacttctttg ccggcgtcag cgctgcgata
     1021 gacgccgtcg agaatttttt ggaccgccag gcccgcttcg ccgggggcgc gaagcgccgt
     1081 gtccttcatg cagccgtcta cgaagttgcg cagcttgctg atgaacaggc tgtggaagtc
     1141 ggtcttgctg ctgaggaagc cgggggtgct gttgatcatg gtgccggcgc gatcggtaaa
     1201 cagggccggc ggatcccagc tggcgccgcc cttgtcgccg accaacgtga agttccacac
     1261 gtccttctca atgtgggcgg cgaacgaggc ttcgatctgc aggatcgcgc cgttctcgaa
     1321 acgaatctgg ccgatcgcca ggtcttccac ggtgtacgtt ttgtaatccc agttgggcca
     1381 ctggctgacg acgtcgctct tcttattgcc catgtacgtc caggtgttgc cgctggcggc
     1441 gaccggtttg ggcgagccca tcacgtagtg cgccatctcg atcacgtgca cgccgatgtc
     1501 gatcatcggc ccgccgcctt gcagatcctt ccggccgaac acgccccagt tgggaatgcc
     1561 gcggcggcga agggcctggc acttgacgaa catgacgttg ccgaattcac cggcttctcg
     1621 cgctcgcgtg aggaattcgg tgttggggtg atagcgatat tgaaaaccga cggccaactt
     1681 cttgccggcc ttcttggccg tgtcgatcat cttctggcat tgcccagggt tcatcgccat
     1741 cggcttttcg acgatgacgt gcgcgccggc gttggatgaa tcgatcgccg gctgcgcgtg
     1801 cacgccgttg ggcgtgcaga tgctgaccgc ttcgggctgg acttccttca gcatcttctt
     1861 ccagtcgctg tagaccttgt ccacgtgcca cttgtcgcgc atgacatcga gccggtccgg
     1921 attgatatcg acaccggcga cgacttccac atccggcatg gtggccagcg cgcctagatg
     1981 ggtttgtgcg atgccaccgc aaccgatgaa cgccacgcgc agttttttgc cggtgtactg
     2041 ctgcttggtt tccatcgcca acgaatcggt cgaaacgctc ttctcggcca tgtgtctgct
     2101 caatcctttc ggggtgtcag gtaatccggt gaacggggcc gatagtttct acagacggcg
     2161 acagaaatgc aacgcgggcg gcgctgggcg ggcgcgcgag ccggctggcg ctggccggct
     2221 ggaatatcac ggagtgatcg cgctcgtcca gccgaccatc cacatgttct gcccgatcgt
     2281 cagcgtgatc agcactaacg cggcataaac ccagattcgc tgccgcgggg accagccggc
     2341 caattcaagc cccaccggca cgagcagcaa cggctgcatg aacagccaga cccgggcggt
     2401 ttccgtctgc aatagtttca gcagcgccac cagcagcggc tgcgcgaggc agagcaggac
     2461 cagcgctgtg tagccttgtc gtttccacgc gcgccatccg attgcgatga ggacggcgct
     2521 gatccagccg ctgcccagca ggaaatcgta cacgtcccac gggatcgatt gcggatatgg
     2581 ccgccgcgcc acctgttcga acaacgcctg ttgatgcagg gcttgtttga acgtcgcgat
     2641 gatgtcgaaa cccgtcgtca accagagcgc gaaccagaag agcacgaacg cgacaacgcc
     2701 ggccacggcg caattgatgg cccaacccca gcgcgcgccc cggcggattt gcaggagagt
     2761 taagccgccg aagaacgcgc cgagcacgag cacgttgaac gcgcaaaagc agcaggccgc
     2821 gagaacgagg ccgaaggcga tggcatatcc gattcgccgg cgaaccaacg cgcggagcca
     2881 cagcaacgcg agcgtcaccg tggcgagggg gtatatcgtg tccaccgctg gaaagaacag
     2941 gacgaagccc gggcacatcg ccagatatga agcggccgcc acaccggcgc tgatcgaacc
     3001 ggtcaactct agaagcagca gataggtcgc cgggatcgac agcgacgcca ggatgccgat
     3061 cgcgagcgaa gcgacgagga tggcctgatg cgcatcgggg aataggccgc ggaatatcga
     3121 aaagaacagg atgacgccgg ggggtctgat gcggctgtgg atgtgcagca tcggcatgat
     3181 ccgcgggaac tgctgcagaa atccgtcgag cgtaatctgc ccgcgctcgc gcgtcgtcat
     3241 cagtacatcg gcatcgacca gatagccgtt ggcgttgccg tcggtcatcg tgcgatacaa
     3301 cgcggcggtg ccgaacggct gcatctgcag gcccgtcgcg atgaacgaca tcgccagcga
     3361 tgacgacatc gacagcaaca gaatccaccg gacgaattgt ggccggcggc gcgccgccag
     3421 tttgatccac aggaactgac cgatgaagaa cggcaccgcg gccgccagca tggatgggta
     3481 aaagcggacg gcgtccaatc gacgccagcg ccagcgccag taccacgggc cgttcacgcc
     3541 aggcgtgtgc ccgcagacca ggacgcaggc gagggcggcg acggcgatga cgagcag
//
LOCUS       DATIMH010000408         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2983689, whole genome shotgun sequence.
ACCESSION   DATIMH010000408 DATIMH010000000
VERSION     DATIMH010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2983689"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..184
                     /locus_tag="VLI90_07030"
     tRNA            111..184
                     /locus_tag="VLI90_07030"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:145..147,aa:Met,seq:cat)
     gene            complement(256..>1655)
                     /locus_tag="VLI90_07035"
     CDS             complement(256..>1655)
                     /locus_tag="VLI90_07035"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSV13997.1"
                     /translation="PEQHPGSIEIAVPPDERVTPAATARVSRRSNRYPALDGIRGIAV
                     LAVMIFHFFLFGKMASIGAVDRVAAGTANAGWAGVDLFFVLSGFLITGILYDARGKHH
                     FLRNFYARRVLRIFPLYYGVLVAFFVVLPHLFPSSAVIHANTLGQLWFWTYLSNVHVA
                     LHGWSATSEYVDHFWSLSIEEQFYVLWPFVIAALSRRSLLRSCAAISIGSLLLRIWLH
                     AHELPNAAFVLTAARMDALAIGAFVALAVRSPAELRTMLRWVRPMLATTTTLLAAIWI
                     ARGGFDKNDPVVGTIGFNLLGLTFGGIILLAIDPAGGGSRMTRLFLSQPLRALGKYSY
                     SLYVFHQPVALVLVSIGISAAAAPVVWGSALPGEIAYAAIAGTDSLIAAMLSWHLFEK
                     HFMKLKDRFAETPVAVPGAPVATAWAVPGVPGGALDDVRLVAARGVVNVAKGDDAGRV
                     NDSKEASGGDFTLGA"
BASE COUNT          327 a          514 c          570 g          244 t
ORIGIN      
        1 cataacccga aggtcgcggg ttcaaatccc gcccccgcta ttgaaaatcg gccggtccgc
       61 gagggccggc cgatttttat gggtgagtgc agaggtgaga aggagttcga ggcagggtag
      121 ctcaggtggt tagagcacgg cactcataat gccggggtcg cgggttcgag tcccgcccct
      181 gctactcagc agacagccca cctggccatc ggccaggtgg gctgtcgtgt atctgccgcg
      241 cgcctccccc caacgttagg cgccgagcgt gaagtcaccg ccgctcgcct ccttggaatc
      301 attcacccga ccggcatcat ctccctttgc aacgtttacg acgccgcgcg ccgcgacaag
      361 acgtacatcg tccagcgctc cacccggaac ccccgggact gcccacgcgg tcgccaccgg
      421 cgcgccaggc accgcaaccg gcgtctcggc gaatcgatcc ttcagcttca tgaagtgctt
      481 ctcgaagaga tgccagctca gcatcgcggc gatcagtgag tcggtgccgg cgatcgcggc
      541 gtacgcgatc tcgccgggga gggcggatcc ccagaccacc ggcgcggccg cggccgagat
      601 gccgatgctg acgagcacca gcgcgactgg ctggtggaag acgtacagcg aatagctgta
      661 cttcccgagg gcgcgtaatg gttgggagag gaagagccgg gtcatgcgcg agccgccgcc
      721 ggccgggtcg atcgcgagca ggatgatgcc gccgaacgtc agtccgagca ggttgaagcc
      781 gatggttccg acgacgggat cattcttgtc gaagccgccc cgtgcgatcc agatcgccgc
      841 gagcagtgtg gtagtcgtcg ccagcatcgg cctgacccat cgcagcatcg tgcgcagttc
      901 ggcggggctt cgcacggcga gcgcgacgaa cgcaccgatg gcgagtgcgt ccatcctcgc
      961 cgcggtcagc acgaacgcgg cgttgggaag ctcgtgcgcg tgcaaccaga tccgaagcag
     1021 caggctgccg atcgagatcg cggcgcagct gcggagcagc gaccggcgcg acagcgcggc
     1081 gatcacgaat ggccagagca cgtagaactg ctcttcgatc gagagcgacc agaagtggtc
     1141 gacgtactcc gaggtggccg accagccatg cagcgcgacg tggacgttcg agaggtaagt
     1201 ccagaaccag agctggccga gcgtattcgc gtggatgacg gcgctggagg gaaacaggtg
     1261 cggcaggact acgaagaagg cgacgaggac gccgtagtac agcgggaaga tccgcagcac
     1321 ccgccgcgcg tagaaattcc gcaggaagtg atgcttgccg cgcgcgtcgt agaggatccc
     1381 ggtgatgagg aatcccgata gcacgaagaa gaggtcgacg cccgcccatc cggcattggc
     1441 cgtcccggcg gcaacgcgat cgacggctcc gatcgacgcc atcttcccga acaggaagaa
     1501 atgaaaaatc atcaccgcga ggaccgcaat gccgcggatg ccgtcgagcg cgggatagcg
     1561 attggagcgg cgtgacactc gcgcagtggc agcgggggtg acacgttcgt cggggggcac
     1621 ggcgatctcg atggagccgg ggtgttgctc gggaa
//
LOCUS       DATIMH010000409         1974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2983789, whole genome shotgun sequence.
ACCESSION   DATIMH010000409 DATIMH010000000
VERSION     DATIMH010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2983789"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..>1974)
                     /locus_tag="VLI90_07040"
     CDS             complement(132..>1974)
                     /locus_tag="VLI90_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSV13998.1"
                     /translation="AAATAGVVGAPIVTAQSPEAAKQEAPVPKSGREKKDIVIERPGS
                     DFMMDVIKTLDLEYMSTNPGSSFRSLQESLVNYAGNRNPELLTCMHEEAAIAMAHGYA
                     KAAGKPMGALVHGTVGLQHGSMAIYNAWVDRVPIMLFTGNGADAVERRPGTEWNHAAQ
                     DVAALVRDFVKWDDSPASLAHFAESTVRAYKIATTPPMEPVLINCDIELQEQPAQHEP
                     KIPKLTRSRPPQGDRTALAEAAKWLVAAEHPVVVADRCVRDQNGVKLLVELAEALQAP
                     VVDLGNRMNFPSVHPLCQTESRRSLIREADVVLLLEVSDSWGQFNSLSDPHHEHRFLG
                     KQGARIVNISMQDVYIRSNYQDFQRYQPADLAINGDAQSSLPALIEEVKRATTDERRR
                     VFGARGEKFKEAHAKEQKRAREEAAIGWNLQPITTARLAAETWAAIKGNDWCLATSDR
                     IPWARRLWPTTSYHQMLGGSGAQGVGYCAPGSLGVALANKSKGILTVTFQPDGDLMYS
                     PGVLWTAAYHKIPLLFVMHNNRAYHQETMHVQRMAGLHNRRPDTAWVGTIIDKPAIDF
                     AKLAQSMGVWAEGPVTDPANLGAALKRAVDVVKRGEPALVDVICQGR"
BASE COUNT          333 a          626 c          637 g          378 t
ORIGIN      
        1 tgagaaaagc gcgcggagaa gagcgaaaag aacgattgat tcaaggtact gctggcaccg
       61 tggataacca ggtcaatcct cagtgctgct tttctcagtg tctccgtgtc tcagtgtcaa
      121 gcagtaatca gttaacgacc ctggcagatg acgtccacca gcgccggttc accgcgcttc
      181 acgacgtcca cggcacgctt caacgccgcg cccaagttcg cggggtcggt caccggtcct
      241 tccgcccaca cgcccatgga ttgcgcgagc ttcgcgaagt cgatcgcggg cttgtcgatg
      301 atcgtgccga cccacgccgt gtcgggacgc cgattgtgca ggcccgccat ccgctggacg
      361 tgcatcgtct cctggtggta tgcgcggttg ttgtgcatga cgaaaagaag cgggatcttg
      421 tgatacgcgg cggtccatag cacgccgggc gaatacatga ggtcgccgtc gggctggaag
      481 gtgacggtga ggattccctt cgacttgttg gcgagcgcga caccgaggga accgggcgcg
      541 caataaccca cgccctgcgc gccggaaccg ccgagcatct ggtggtatga cgtcgtgggc
      601 cagagccgcc gagcccaggg aatgcgatca ctcgtcgcga ggcaccaatc gttgcccttg
      661 atcgcagccc acgtttcagc ggcaaggcgc gcggtcgtga tgggttgcag gttccagccg
      721 atcgccgctt cctcgcgagc acgcttttgc tccttcgcgt gcgcttcctt gaacttctca
      781 ccccgcgctc caaatacgcg gcgccgttcg tccgtcgtgg cgcgcttcac ttcttcgatg
      841 agcgccggca agctcgactg cgcgtcgcca ttgatcgcga ggtcggccgg ctggtagcgc
      901 tggaagtcct ggtagttgga gcggatgtag acgtcctgca tcgaaatgtt cacgatgcgc
      961 gcgccctgct tcccgaggaa gcgatgctcg tgatgaggat cggagagcga gttgaactgg
     1021 ccccatgaat cggagacctc gagaagcagc acgacgtcgg cttcacgaat gagcgaacgc
     1081 cgcgactcgg tctggcaaag cgggtggacg ctcggaaagt tcatgcggtt gcccaggtcg
     1141 acaaccggtg cctgcagcgc ttcggcgagc tcgaccagca gcttcactcc gttctggtcg
     1201 cgcacgcagc ggtccgcgac gacgacggga tgctcggcgg cgacaagcca tttcgccgct
     1261 tcggccagcg cggtgcgatc gccctgcggc ggacgcgaac gggtgagctt cggaatcttc
     1321 ggctcatgct gcgccggttg ctcctgcagc tcgatgtcgc agttgatgag caccggctcc
     1381 atcggcggcg tggtcgcgat cttgtacgcg cgcaccgtcg attcggcgaa atgggcgagc
     1441 gacgcgggcg aatcgtccca cttcacgaag tcgcggacca gcgccgcgac gtcttgagcc
     1501 gcatggttcc attcggtgcc ggggcgccgc tcgaccgcat cggcgccgtt accggtgaag
     1561 agcatgatcg gcacgcggtc gacccacgcg ttgtagattg ccatggagcc gtgctgcagc
     1621 cccaccgtgc cgtgcaccag cgcgcccatg ggtttgccgg cggccttggc atagccatgc
     1681 gccatcgcga tagccgcttc ctcatgcatg caggtgagaa gctcgggatt cctgtttccc
     1741 gcgtaattca ccagcgactc ctggaggctg cggaagctcg accccggatt cgtcgacatg
     1801 tactcgagat cgagcgtctt gatgacgtcc atcatgaagt cggagccggg tcgctcgatc
     1861 acgatgtctt tcttctcccg gccactcttt ggcaccgggg cctcctgctt cgcggcctcg
     1921 ggtgattgag ccgtcacgat cggagctccg acgacgcccg ccgtggcggc ggca
//
LOCUS       DATIMH010000410         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_676743, whole genome shotgun sequence.
ACCESSION   DATIMH010000410 DATIMH010000000
VERSION     DATIMH010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676743"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..2196)
                     /locus_tag="VLI90_07045"
     CDS             complement(196..2196)
                     /locus_tag="VLI90_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV13999.1"
                     /translation="MLKLIRNHQKKFLAFFGVGLMITFVVQTGYSRGGGSGGRRDVTV
                     GTVNGNKPILSSELEEAKHEWRAVQQYVLIPVGGQYSQQRIPYTQVAFGRDPAFGQQM
                     SAMIEQKPEVLALLRREARDAGVRADENEVETILANYYTNTGNDDINYLVRQGVTDIA
                     LIEANFNRIASDIKISAPLREKVLARVLQLIQVNALELSANDYANKVPPPTTQQIEDQ
                     FTKYADVAPPQQPDPKNPFGFGYRLPDRVKVQYLAIDRAQVQKAVERKMSPYEWDVQA
                     RLYYRQHKDEFPTTQPAPPTTQSTAVSAATSQPSTAPAYRPYNEVAKDALRDVQDPMV
                     DKLMFDIQTKITAQLANDWRTYQQSAASAGAGASTAPSATAAGDYPSYKYLKKLADSI
                     QKQFDVTVFANDFDRFYSADELNTLPGIGQLGETAFGRAGVKFSDVAMQRAAPYLSGS
                     ADKRTAAARAQLMQPSPPLPDATRDVYIFRLTDARPAEPATDLNEVRAKVEMDLRKAA
                     GYDVAKAAAKPALEAAQSGSLNAEAMAMGKRVITTDPFNSNPYTNNPPTLGGGINLSP
                     AAARDFAEQAFGLLAQYNPTTNAHPVKLIELPEDGKLYVAQLTNITPQWDTGTFYSRW
                     LDVSAGLRQGLANGLRSDWFNYDAVIQRTGYKPDKAQQASAD"
BASE COUNT          350 a          632 c          791 g          495 t
ORIGIN      
        1 gacaacaaca tccttagcgc gccagggccg ccatttgttc gcgctagtgt tttaaaagtg
       61 gggatgactc aggcgttcat cgtctgcatg acgttgcgta gcggtcgccc cgaaggggca
      121 cggcttttct gttgtcgaac ggcaaaaacg cgtgcccctg tggggctacc gctacgcggg
      181 gtcgttcgcg ctgtcctagt ccgcggaggc ttgttgcgcc ttgtcgggtt tgtagccggt
      241 gcgttgaatg acggcgtcat agttgaacca gtcactgcgc aggccgttgg ccaagccttg
      301 gcggaggccg gcgctgacat cgagccagcg tgagtagaac gtgcccgtgt cccattgcgg
      361 cgtgatgttc gtcagctgtg cgacgtacag cttgccatcc tcgggcagct cgatgagttt
      421 aaccggatgc gcattcgtcg ttgggttgta ctgcgcgagc aggccgaagg cttgctcggc
      481 aaagtcgcgc gcggcggcgg gggacaggtt gatgccgccg ccgagcgtcg gcgggttgtt
      541 cgtgtaagga ttgctgttga acggatcggt cgtgatcacg cgtttgccca tggccatggc
      601 ttcggcgttg agactgccgg attgagcggc ttcgagcgcg ggcttggcag cggccttggc
      661 cacgtcgtaa ccggcggctt tgcgaaggtc catttccacc ttggcgcgga cttcgttcag
      721 atccgtcgcc ggctcggcgg ggcgggcgtc ggtcaggcgg aagatgtaga cgtcgcgcgt
      781 ggcatcgggc agcgggggcg acggttgcat cagctgcgcg cgagcggccg cggtgcgctt
      841 gtcggcgctg ccgctgagat acggggcggc acgctgcatg gcaacatctg agaacttcac
      901 gccggctcgg ccgaaggcgg tttcacccag ctggccgatg ccgggcagcg tgttgagttc
      961 atcggcggaa taaaagcgat cgaaatcgtt ggcgaagacg gtgacgtcaa actgcttctg
     1021 gatagagtcg gcgagcttct tgagatactt gtaagacggg taatcccccg cggcggtggc
     1081 tgagggcgcg gtcgatgcgc cggcgcccgc gctggcggcg ctttgctgat acgtgcgcca
     1141 gtcgttggcc agctgcgcgg tgatcttcgt ctggatgtcg aacatcagct tatcgaccat
     1201 cggatcctgc acgtcgcgca gcgcatcctt ggcgacttcg ttgtacggtc gatacgccgg
     1261 cgccgtggac ggctgcgaag tagctgcgga aacggcggtt gactgcgtgg ttggcggcgc
     1321 cggttgcgtc gtcgggaatt cgtccttgtg ctggcgatag tagagccgag cctgcacgtc
     1381 ccattcgtac ggcgacatct tccgttcgac ggccttctgc acctgagcgc gatcgatcgc
     1441 caggtactgc accttcaccc gatcgggcag gcggtagccg aagccaaacg ggttcttcgg
     1501 gtccggctgt tgcggtgggg cgacgtcggc gtacttcgtg aactggtctt cgatctgctg
     1561 cgtcgtcggc ggtgggactt tgttcgcgta atcgttggcc gagagctcga gcgcgttgac
     1621 ctggatgagc tgaagcacgc gggctaagac cttctcgcgc agcggggcgc tgatcttgat
     1681 gtcgctggcg atgcggttga agttggcttc gatcagcgcg atgtcggtga cgccttggcg
     1741 caccagatag ttgatgtcgt cgttgccggt gttggtgtaa tagttggcca ggatcgtttc
     1801 gacttcgttc tcgtcggcgc gcacgccggc gtcgcgcgcc tcgcggcgca gaagcgccaa
     1861 gacctcgggc ttctgctcaa tcatggcgga catctgctga ccgaacgcgg ggtcgcggcc
     1921 gaaggcgacc tgcgtgtatg ggatccgctg ctggctgtac tgcccgccga cggggatcag
     1981 cacatattgc tgaaccgcac gccattcgtg cttggcttct tccagttcgg aagagaggat
     2041 gggcttgttc ccgttgacgg tgccgaccgt gacgtcgcgc cggccaccgc tgccgccccc
     2101 gcggctgtag ccggtctgga ccacgaacgt gatcatcaac ccgacgccaa agaaggcgag
     2161 gaatttcttt tggtgattgc gaatgagttt gagcatttgg ctttgttttc gagtttatcc
     2221 tgagcgaagt ggcaaagccg tgcagtcgaa ggatctggct tcgaacgt
//
LOCUS       DATIMH010000411         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3272801, whole genome shotgun sequence.
ACCESSION   DATIMH010000411 DATIMH010000000
VERSION     DATIMH010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272801"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VLI90_07050"
     CDS             <1..319
                     /locus_tag="VLI90_07050"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HSV14000.1"
                     /translation="GGFKFRRQHPKPPFIADFYCAEVKLVLETDGDSHAEREEFGASR
                     TVRLERDGLHVIRFVNDDVHRHLDSVLEEIPGECERLAKQNHPHPNPLPEYRERGPEQ
                     TCY"
     gene            310..1278
                     /locus_tag="VLI90_07055"
     CDS             310..1278
                     /locus_tag="VLI90_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HSV14001.1"
                     /translation="MLLKLTHTTDLSYSDLVNESVMELRMAPRQEQDQHRLSFALAIG
                     PATQVTSYFDWLGNTVHTFTIGPFHKQIRIVATSVVETDRPRPEVQRFSDIWPLAAGA
                     SDDYALYDYLHFGGPIVDVPPLHALARVLQPTPGLSLGELALRMLHLMNEKFIYKTGI
                     TSAASPITDMLDSGAGVCQDFTHLMIGLARALGIPARYVSGLIHPDAERYRGFTQTHA
                     WCELYFPSAGWVGFDPANNCIIGANFVKVAVGRDFRDVPPNKGLYRGNAQESIDVKVQ
                     SEELRLVPAELAAERVLPLGIPTYPAGFSIHREMAEQQVEVQQQQQ"
     gene            1548..>2969
                     /gene="xylB"
                     /locus_tag="VLI90_07060"
     CDS             1548..>2969
                     /gene="xylB"
                     /locus_tag="VLI90_07060"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HSV14002.1"
                     /translation="MPYLLGIDIGTSGTKTLICDDDGKVLATAMAEHPISSPKPGWSE
                     QDPQDWWAATIKATRAVLKKAKVKATDIGGIGLSGQMHGSVFLGDGPKPLRPALLWND
                     QRTAKQCAEIESKAGGRAALIELVANPALTGFTAPKILWVRENEPKVYAKTRHILLPK
                     DYIRYLMTGDYATEVSDASGTLLLDVVNRTWSDKLLGLLQIDRALLPRLHESPQVTGG
                     LTAKAAEALGLREGTPVVGGAGDQAAGAVGNGIVMSGIVSATLGTSGVVFAHSDSPTR
                     DPLGRVHTMCHAVPGKWCVFGCMLSAGGSFQWFRNQMAQTEVAQAKKKKIDPYELLIA
                     EARTAPPGSEGTFFLPYLTGERCPHPDPNARGGWIGITARTDRAMLIRSLLEGVTYGI
                     RDALEIMNTMGCAISQVRASGGGARSDFWQHLQADIYQQPIVLTNAAEGPAYGVALLA
                     GVGTGVWHSVEEACRASIKSVKKI"
BASE COUNT          562 a         1000 c          919 g          488 t
ORIGIN      
        1 cggcgggttt aaattccgcc ggcaacatcc taaaccgccg ttcatcgcgg acttttattg
       61 cgcggaagtg aaactggttt tggaaaccga tggcgactcg catgcggagc gcgaggagtt
      121 cggcgcgtcg cgcacggttc gcttggagcg cgatggactg cacgtcatcc gcttcgtgaa
      181 cgacgacgtt catcgacatc tggattcggt gctggaggag attcctgggg aatgtgaacg
      241 tctcgcaaaa caaaatcacc ctcaccctaa ccctctcccg gagtaccggg agaggggacc
      301 agagcaaaca tgctattgaa gctgacccac accaccgacc tctcttactc cgacctcgtc
      361 aacgagtccg tcatggaact ccgcatggcc ccgcggcagg agcaggacca gcatcgcctc
      421 tcgttcgcgc tggcgatcgg gcccgcgacg caagtcacca gctacttcga ctggctcggc
      481 aacaccgtgc acaccttcac catcgggccg ttccataagc agatccgcat cgtggcgacc
      541 agcgtcgtcg agaccgaccg cccgcggccg gaggtgcagc gcttcagcga catctggccg
      601 ctggcggcgg gggcgagcga tgattacgcg ctgtacgact acctgcattt cggcgggccg
      661 attgtcgatg tgccgccgct gcacgcgctg gcgcgagtgc tgcaaccgac gccggggttg
      721 tcgctggggg agttggcctt gcgcatgctg cacctgatga acgagaagtt catctacaag
      781 acgggcatca cgtcggcggc cagcccgatc acggacatgc tcgacagcgg cgccggcgtt
      841 tgtcaggact tcacgcatct gatgatcggc ctggcccgcg cgctggggat tccggcgcga
      901 tacgtcagcg ggttgatcca tccggacgcc gagcgctatc gcgggttcac gcagacgcac
      961 gcgtggtgcg agctgtattt tccgtcggcg ggatgggtgg ggttcgaccc ggcgaacaac
     1021 tgcatcatcg gcgcgaactt cgtgaaggtg gcggtcggcc gggatttccg cgacgtcccg
     1081 ccgaacaagg ggctgtaccg cgggaacgcg caggagtcga tcgacgtgaa ggtgcagagc
     1141 gaagagctgc ggctggtgcc ggcggagctg gcggcggaac gcgtgttgcc gctgggcatc
     1201 ccgacgtacc cggcggggtt ctcaattcac cgagagatgg cggagcagca ggtcgaggtg
     1261 cagcagcagc agcagtgatc atttgtcccg cgcgccttgc cccctctccg gtacgccggg
     1321 cgagggctgg ggtgagggtg atcttgagtg aggaaattcg aatggcgaaa tccgaatccc
     1381 cgaatgaatg acgaaatccg aatgacgaat ggatcgatgc ttgcgcgatc gacgtttggt
     1441 cattcgttat tcgccattcg gatttgattc ggggattcgg gtttcggaat tcgagcttca
     1501 ctgacatcct cttgcgtctc ccccggcact cgccgaatat ccaccccatg ccctacctgc
     1561 tcggcatcga catcggcacc agcggcacca agacgctcat ctgcgacgac gacggcaaag
     1621 tcctcgccac cgccatggcg gagcatccca tctcctcgcc caagcccggc tggtccgagc
     1681 aggacccgca ggattggtgg gccgcgacga tcaaggcgac gcgcgcggtg ctgaagaagg
     1741 cgaaggtgaa agcgacggac atcggcggca tcggcctgtc cggccagatg cacggcagcg
     1801 tcttcctcgg cgatggtccg aaaccgcttc ggcccgcgct gttgtggaac gatcagcgca
     1861 cggccaagca atgcgccgag atcgaatcca aagccggcgg tcgcgccgcg ctcattgagt
     1921 tagtcgccaa tcccgcactg actggtttca ccgcgccgaa aatcctctgg gtccgcgaga
     1981 acgagcccaa ggtctacgcc aagaccaggc acatcctgct gcccaaggat tacatccgct
     2041 acctgatgac cggcgactac gcgacggaag tcagcgacgc cagcggcacc ctgctgctcg
     2101 acgtcgtcaa ccgcacctgg tccgacaagc tgctcggact tctgcagatc gaccgcgcgc
     2161 tgctcccgcg cctgcacgaa tccccacagg tgacaggcgg actgaccgcc aaggccgccg
     2221 aggcgctggg cctgcgcgaa ggcacccccg tcgtcggtgg cgccggcgac caggccgccg
     2281 gcgcggtcgg caacggcatc gtcatgtccg gcatcgtcag cgcgacgctc ggcaccagcg
     2341 gtgtcgtctt cgcccatagc gactcaccga cgcgcgatcc cctcggccgc gttcacacca
     2401 tgtgccacgc cgtccccggc aagtggtgcg tcttcggctg catgctctca gccggcggca
     2461 gcttccaatg gttccgcaac cagatggcgc agacggaagt cgcgcaagcg aaaaagaaga
     2521 agatcgaccc gtacgaacta ctgatcgccg aagcccgcac cgccccaccc ggcagcgaag
     2581 gcacgttctt cctcccctac ctcaccggcg agcgctgccc gcatcccgac cccaacgccc
     2641 gcggcggatg gatcggcatc accgcccgca ccgaccgcgc catgctcatc cgctccctcc
     2701 tcgaaggcgt cacctacggc atacgcgacg ccctggaaat catgaacaca atgggctgcg
     2761 ccatctccca agtccgcgcc tccggtggcg gcgcccgcag cgacttctgg caacacctgc
     2821 aagccgatat ctaccaacaa cccatcgtcc taaccaacgc cgccgaaggc cccgcctacg
     2881 gcgtcgcgct gctcgccggc gtcggcaccg gcgtctggca cagcgtcgaa gaagcgtgcc
     2941 gcgcgtcgat caagagcgtg aagaagatc
//
LOCUS       DATIMH010000412         5626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3848726, whole genome shotgun sequence.
ACCESSION   DATIMH010000412 DATIMH010000000
VERSION     DATIMH010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3848726"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2623)
                     /locus_tag="VLI90_07065"
     CDS             complement(<1..2623)
                     /locus_tag="VLI90_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucokinase"
                     /protein_id="HSV14003.1"
                     /translation="MKAVASPNLGRLNVALDANAAAYRRAVQSSAPGECWDFVVLTAA
                     NEKQAQGYRQELALRQRAVGPTGAFFPAIQRSIVIADPPGRRVGSGGATFRVLRKLVE
                     QFKLRESDLDRLHILIIHSGGASQHLPAYAPLGKIFAPLPLMRPDGQIATLFDHLYLT
                     LAGLPDRLGPGMLVLAGDVFLLFDHRQVLPPSPGVTALPMRVDAELGREHGVFVTDPA
                     GAITRTLQKVPIETLREARAIDTHGRILIDTGLLFFDPPRVNTLAKLAACGFADRKAP
                     ARKIGIDARHGIPIDLYEDITGALATDTTRKDFLADARTRPMRSEIFRAVHGVPFHAA
                     KLEGEFLHLGTTRQFRDAMIGRAHSPAAELFQQNVLARSEWHLDTGRRVYHSVLLANQ
                     DRSRSGVLGDGSVVEHSLVRAPSKVGRGSVVSQVIAVDRSIELGEDLLLFQVPVRSEF
                     GAPAFVHVLCGVEDDFRGTFADGQCTYLNEPIDQWLHRHGVTPDELWPDVPARQRTLW
                     NARLFMATSKRDASDAALRGGNGRATKASIAAWRKSPRYSMAMVLEQADPSALIEHRE
                     MISAYLQTSEMLESIRRREDKPLASFVTHFSTAAAYEASERLLEDWAAEPTANSNVAI
                     QQARSLWCVAQLLQRPDHPASSDGRPRIEDYMDRAFAKVASASEIGHANIEVRGRPGK
                     LLRPGTEILATSPVRIDLAGGWSDTPPYCFEFGGHVINVALDLDGRPPVRATVRILRE
                     PKVVLESHDLGQTIELTEADAAQPINVRDPFALHKIALDITGFLPRRPERLRHRLQLL
                     GGGLQITTECRVPKGSGLGTSSILAATLLGALHKSRRHDANIDELSNKRCCWSSASAP
                     AEGRRTKSAASSAGQSPP"
     gene            complement(2620..3927)
                     /locus_tag="VLI90_07070"
     CDS             complement(2620..3927)
                     /locus_tag="VLI90_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV14004.1"
                     /translation="MPDSVPQPKTVLVVSQTFVPDPASVGQHMADVAFELSRRGHRVR
                     VYASGRGYEDSTVVYPRREMLNGAEIRRFPFASFGKKSILLRVLGTAVFQIQAFFAAL
                     FTRRLAGIFFSTSPPLIGLPLCIAAAIRRVPTAYWAMDLNPDQLIALGKLKPTDFVAR
                     LLEWANRFILHHVSLIVALDRFMAERLAARGIASERMLILPPWPHEDQIHDETAIDRE
                     QNPFRQRHNLRNKFVIMYSGNHSPSNPLTTLLDAAVRLKNDPNLMFLFVGGGNGKREV
                     EAYIRDHQLTNALSLPYQPLSELKYSLTAADVHVVALGDPMVGIIHPCKIYGAMTAAK
                     PVLFLGPRPSHISDLLDQHGIGLHVAHGDVAGAIAAIERLRTMPATEREAMGRTAESV
                     LHRSLSQKYLCGRFCDATEAALGLRAEPTSSTTPPLPEAAAKP"
     gene            complement(4122..4670)
                     /locus_tag="VLI90_07075"
     CDS             complement(4122..4670)
                     /locus_tag="VLI90_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14005.1"
                     /translation="MAYATFGPVRTAPKTFAETVQSRRYMTVMNLQEQAEALRLLLLM
                     GVADKAELVAWADHIITTQDGVPGWLLDLSLGANEDAAAIEAKLRDLPGECKARSAAY
                     AAMDRFAQEFQVKGKFTSRQAANMLTIWACSAKIEEEDRLAAMMPYYIADEVPFGNAT
                     DQDVVDAIDECIARFATLSASA"
     gene            complement(4776..>5626)
                     /locus_tag="VLI90_07080"
     CDS             complement(4776..>5626)
                     /locus_tag="VLI90_07080"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HSV14006.1"
                     /translation="LTREQDAERLYTEFKPTVVLHLAAEVGGIGANRDNPGRFFFANL
                     AMGMHLIEGARKFGLKKFVQVGTICAYPKFTPVPFRENELWNGYPEETNAPYGIAKKS
                     LLVMCQAYRQQYGLNAIYLLPVNLYGPRDNFHLHSSHVIPALIRKCVEARRRGDAQIT
                     AWGTGSASREFLYVEDCAEGLVAAMEQYDSPEPMNLGSGREITIKNLTELIAKISRFE
                     GQIIWDTTKPDGQPRRCLDVARAKQTIGFTARTTLEDGLRKTIEWFEAHIDAIPERAF
                     SAPAAR"
BASE COUNT          955 a         1698 c         1909 g         1064 t
ORIGIN      
        1 acggtggact ttgccccgcc gacgatgccg ccgacttggt cctgcgaccc tccgccggtg
       61 ctgaggcgct gctccagcag cagcgtttgt tcgatagctc atcgatgttt gcatcgtgcc
      121 ggcgagattt gtgcagcgcc ccgagcagcg tcgcggcaag gatgctgctg gtgcccagcc
      181 ccgagccttt gggcacgcgg cactcggtgg tgatctgcaa tccgccgccg agtaattgca
      241 ggcgatgccg caagcgctcc ggtcgacgcg gcaggaagcc ggtaatatcg agcgcgatct
      301 tgtgcagcgc gaaggggtcg cgcacattga tgggctgcgc cgcatccgcc tcagtcagct
      361 cgatcgtctg gcccaggtcg tgactttcca gcaccacctt cggctcacga aggatgcgca
      421 cggtcgcgcg caccggtggg cggccgtcca gatcgagcgc gacgttgatc acgtgcccgc
      481 cgaactcaaa acagtatggt ggcgtgtcac tccacccgcc ggcgagatcg attctgaccg
      541 gcgatgttgc gaggatttcg gtgccggggc gcagcagttt gccgggccgc ccgcgcactt
      601 cgatgttggc gtgaccgatc tcgctggcgc tagcgacttt ggcaaacgcg cggtccatgt
      661 aatcttcaat gcgcggccgt ccgtcgctgg acgccggatg atccggccgc tgcagcagct
      721 gtgcgacaca ccacagcgat cgcgcctgct gaatcgccac gttcgaattc gccgtcggct
      781 cggccgccca gtcttccagc aaccgctcgc tggcttcgta cgccgccgcg gtcgagaaat
      841 gcgtgacgaa agacgcgagc ggtttgtctt cgcggcggcg aatcgattcg agcatctcgc
      901 tggtctgaag gtacgccgag atcatctcgc gatgttcgat cagcgcactg gggtccgcct
      961 gctccagcac catcgccatg gaataccgcg gcgactttcg ccaggcggcg atggacgcct
     1021 tggttgccct gccgttgccg ccgcgtaatg cggcatccga tgcgtcgcgc ttggacgtcg
     1081 ccatgaacag ccgggcgttc cagagcgtgc gttgccgcgc tggaacgtcc ggccagagtt
     1141 cgtccggcgt cacgccgtgg cgatgcagcc actgatcgat cggctcgttg agatacgtgc
     1201 actgcccgtc ggcgaacgtc ccgcgaaaat catcttcgac gccgcataag acatgcacga
     1261 acgcgggggc gccgaattca cttcgcaccg gaacctgaaa cagcagcagg tcttcgccca
     1321 gttcgatcga gcgatcgact gcgatcacct ggctgacgac gctgccgcgg ccgactttgg
     1381 atggggcgcg cacgagactg tgttcaacga cgctgccgtc accgagcacg ccggagcggc
     1441 tgcggtcctg attcgccagc agcacgctgt gatacacgcg gcggccggtg tcgagatgcc
     1501 attcgctgcg ggcgagcacg ttctgttgga acagttcggc cgcgggggag tgggcgcggc
     1561 cgatcatcgc gtcgcggaat tggcgggtgg tgccgaggtg caaaaactcg ccttcgagct
     1621 tggcggcatg gaagggaacg ccgtgcacgg cgcggaagat ttcgctgcgc atcgggcgcg
     1681 ttcgcgcgtc ggcgaggaaa tcctttcgcg tcgtgtcggt ggccagggcg ccggtaatat
     1741 cttcatagag atcgattggg ataccgtggc gggcgtcgat tccgattttg cgagccgggg
     1801 cttttcggtc tgcgaagccg caagcggcta gcttcgcgag cgtgttgacg cgcggcggat
     1861 cgaagaacag caggccggtg tcgatcagga tgcggccgtg cgtgtcgatc gcgcgcgctt
     1921 ctcgcagcgt ttcgatcggg accttctgca aggtgcgtgt gatcgcgccg gcggggtcgg
     1981 tgacgaagac gccgtgctcg cggcccagtt ccgcgtcaac gcgcattggc aacgccgtca
     2041 cgccaggcga cggtgggagg acctggcggt gatcgaagag caggaacaca tcgccggcga
     2101 ggacgagcat gccggggccg agacgatcgg gtaatccggc gagcgtcagg tacaggtgat
     2161 cgaacagcgt ggcgatctgg ccatcgggcc gcatcagcgg cagcggcgcg aaaatttttc
     2221 cgagcggcgc gtacgccggc agatgctggc tcgcgccacc gctgtggatg atcaggatgt
     2281 gcaggcgatc gaggtccgac tcgcgcagtt tgaattgctc gaccagcttg cgcagcaccc
     2341 tgaacgtcgc cccgccgctg ccgacgcggc ggccgggcgg atcggcgatg acgatgctgc
     2401 gctgaatcgc cgggaagaac gcaccggtgg ggcccaccgc gcgctggcga agcgccagtt
     2461 cctgtcgata tccctgcgcc tgtttttcgt tggcggcggt gaggacgacg aagtcccagc
     2521 attcgccagg ggcgctgctc tgcaccgccc ggcgatacgc ggcggcgttg gcgtcgagcg
     2581 cgacgttcag ccggcccagg ttgggtgatg cgaccgcttt catggcttgg ccgccgcctc
     2641 tggcagcgga ggcgttgtgg aggatgtggg ttcggcgcgc aagccaaggg cagcttcggt
     2701 cgcatcgcag aatcggccgc agagatattt ctgactgagc gatcggtgca gcacgctttc
     2761 ggccgtccga cccatcgctt ctcgctcggt tgccggcatg gttcgcaggc gttcgatcgc
     2821 cgcgattgcg ccggcgacat ccccgtgcgc gacatgcagg ccgatgccgt gttgatcgag
     2881 caggtcgctg atgtgcgacg gccgcggacc caggaacagc accggtttgg cggcggtcat
     2941 cgcgccgtag atcttgcacg ggtggatgat gccgaccatc ggatcgccca gcgcgacgac
     3001 gtgcacgtcc gcggcggtca gcgagtattt caattcgctc agcggctggt atggcagcga
     3061 cagcgcgttg gtcaactgat gatcgcgaat atacgcctcg acttcacgct taccgttgcc
     3121 gccaccgacg aacaggaaca ttaggttcgg gtcgttcttc agtcgcacgg cggcgtccag
     3181 cagcgtcgtc agcgggttgc tggggctgtg attgccgctg tacatgatga cgaacttgtt
     3241 cctgagattg tgccgctggc ggaaggggtt ttgctcgcga tcgatcgccg tctcgtcgtg
     3301 gatctgatct tcgtgcggcc aaggtggcag gatgagcatt cgctcgctgg cgatgccgcg
     3361 cgcggctagg cgctcggcca tgaatcgatc gagcgccacg atcagagaca cgtgatgcag
     3421 gatgaaacgg ttggcccatt ccagcagccg cgcgacgaag tcggtcggct tgagcttgcc
     3481 cagtgcgatc agttgatccg ggttcaaatc cattgcccag tacgcggtgg gcacccggcg
     3541 aatcgcggcg gcgatgcaca gcggcagccc gatcagcggc ggcgatgtgc tgaagaaaat
     3601 cccggcgagc cggcgtgtga acagcgctgc gaagaacgcc tggatctgaa ataccgctgt
     3661 gccgagcacg cgcagcagga tggacttctt cccgaacgaa gcgaacggaa agcggcggat
     3721 ctccgcgccg ttcaacatct cccgccgcgg gtaaacgacg gtcgaatctt catacccgcg
     3781 gcccgaggca tacacgcgca cgcggtgccc gcggcgtgac agttcgaacg ccacatcggc
     3841 catgtgctgc ccgacgctgg ccgggtccgg cacgaacgtt tggctgacga cgagaacggt
     3901 cttgggctgg ggaacagaat cgggcatgcg tttccagtgc agcggattat gaatcgggca
     3961 gcgtgggggt caaggcggag cgacgcgcgg gatttgaaag agatgcgaaa cgcgcagaaa
     4021 cccggatgcg atgcgtagcg gtagccccga aggggcacgt gtttcgggtt cgcgcggaaa
     4081 cacgtgctgc ggggttacca atacgcggtg gaagtggggc gctacgctga tgcgctcaac
     4141 gtggcgaagc gggcgatgca ttcatcgata gcatcgacca cgtcctgatc cgtggcattg
     4201 ccgaaaggga cttcatccgc aatgtaatac ggcatcattg cggccaatcg atcttcttct
     4261 tctatcttcg cgctgcaggc ccagatcgtg agcatgttcg ccgcctgccg cgaggtaaac
     4321 ttacctttga cttgaaactc ctgagcgaat cgatccatgg ccgcgtatgc ggccgatcga
     4381 gccttgcact cgccgggcaa gtctcgcaat ttggcttcga tcgccgccgc gtcttcgttg
     4441 gcgccaaggc taagatccag taaccacccg ggcacgccgt cttgcgtagt gatgatgtgg
     4501 tccgcccacg cgacgagttc cgctttgtcg gccacgccca tgagcaggag cagccggagt
     4561 gcctcagctt gttcttggag gttcatgaca gtcatatagc ggcggctttg cacggtttct
     4621 gcaaacgtct tgggagctgt ccgcacagga ccgaacgtcg cgtatgccac gccgggctcg
     4681 gagcgtactc gcgacgaccc ggatgcttgg gcgcgcgaga aggatccggg tcgtcggagt
     4741 acctccgaac ccggcgtggg tgcctatata gatcgctatc ttgccgcggg ggcgctgaag
     4801 gcacgctcag ggattgcgtc aatgtgcgct tcaaaccact cgatcgtctt ccgcagcccg
     4861 tcttccaacg tggtacgtgc ggtgaagccg atcgtttgct tcgctcgggc gacgtccaga
     4921 caccgccgcg gttggccgtc gggcttggtc gtgtcccaga tgatctggcc ttcgaagcgg
     4981 ctgatcttgg cgatcagctc cgtcaggttt ttgatcgtga tctcgcggcc gctgccgagg
     5041 ttcatcggct cgggcgaatc gtattgctcc atcgcggcga ccaggccttc ggcgcagtct
     5101 tcgacgtaga gaaattcacg gctggccgag ccggtgcccc aggcggtgat ctgcgcatcg
     5161 ccgcggcggc gggcttcgac gcacttgcgg atcagcgcgg ggatcacgtg gctagagtgc
     5221 aggtggaagt tgtcgcgcgg gccatacagg ttcacgggca gcaggtagat cgcgttcagg
     5281 ccgtattgct ggcgatacgc ctggcacatg accagcagcg attttttagc gatgccgtac
     5341 ggcgcgttgg tctcttcggg gtagccgttc cacagttcgt tttcgcgaaa gggaaccggg
     5401 gtgaacttgg gatacgcgca gatggtgccg acctggacga actttttgag accgaacttt
     5461 cgcgcacctt cgatgaggtg catgcccatg gcgaggttgg cgaagaagaa gcggccgggg
     5521 ttgtcgcgat tggcgccgat gccacccact tccgccgcca ggtgcagcac gacggtcggc
     5581 ttaaactcgg tgtagagacg ctcagcgtcc tgctcgcgcg tgaggt
//
LOCUS       DATIMH010000413         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2984460, whole genome shotgun sequence.
ACCESSION   DATIMH010000413 DATIMH010000000
VERSION     DATIMH010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2984460"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VLI90_07085"
     CDS             <1..239
                     /locus_tag="VLI90_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HSV14007.1"
                     /translation="ELLNQMTADACGRRVITGPVEATAIGNILVQAMAIGAVKSLADA
                     RTIVRDSFEVKTYEPRETKKWDGMYARFREIVQQ"
     gene            complement(710..841)
                     /locus_tag="VLI90_07090"
     CDS             complement(710..841)
                     /locus_tag="VLI90_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14008.1"
                     /translation="MYHQAHSIDRARFHLLIEDAVVAIDAVEGEDHRRVEFRVPSIV"
     gene            914..>2113
                     /locus_tag="VLI90_07095"
     CDS             914..>2113
                     /locus_tag="VLI90_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HSV14009.1"
                     /translation="MNRREMLAALGLVTAGGVVGCSPLAATAAAGLTPPDPRKIFDVK
                     AYGATGDGKTLDTPAINRAIDACSSGGGGMVYVPPGTYLCGTIQLKSNVTLFLEAAAI
                     IQGSSRSEDFGLMTGPPEQGDMNQRHVIFARDASNITLAGTGTIDGNGAAYWTQRARP
                     ATDPWGDVATYDYVASRRRPSPLLEFYNCTDLGIRGVHIRNAPGWTLRPIQCSNVFID
                     GISIKNPIYGLNTDGIDVTCCKNVFISNCLIETGDDTICLKSESPYGGEPAVTKNVTI
                     TNCVLSCCCNGLKIGTATHGGFENIVFSNSVIFNEDVPINQRVIGGVSLEVVDGGYID
                     GVLVSNIRMQRVRAPIFIRRGARKGPTDNKVNYLRGIMIENVHATGAIMTSSVTGLPG
                     ANVEDVTL"
BASE COUNT          458 a          653 c          640 g          362 t
ORIGIN      
        1 acgagctact gaaccaaatg acggcggacg cctgcgggcg gcgagtgatc accggcccag
       61 tggaagcgac cgcgatcggc aacatattgg tccaagccat ggcgatcggc gcggtcaaat
      121 ccctcgccga tgcgcgcaca attgtgcgcg atagcttcga ggtcaagacc tacgagccgc
      181 gcgagacgaa gaagtgggat gggatgtacg cgcgctttcg ggagatcgtc cagcagtaga
      241 ttcgcgcatc gacgcgacca acgccggcag ctcagcgcga gaccgggcaa agcaaaagca
      301 gcggcatgag ttacaagctg ggaatatcga accccaccgc caaagaggcg tagacggcgc
      361 atcagcgtcc gcatgatcaa ggctacggtc ctcttagcgg ccctgatctg ggttggattt
      421 atcgtctcct ggatttatgc ggattacgaa gcccataagt gggactgtca ccgccgagat
      481 atttatcaag tggcaccggg aatcccgccg aggcattgag tgcacggctc gtgcagacaa
      541 taaaagagca tggttatcga cgcccacgtt cacgtctgcg ccatgagccc cggtcatggc
      601 ttcagtctca gcggctcatc gacagcctcg ccttccgctt catgcgctgg caccttggca
      661 tcgtcgaccg cgacgaacgc accgaacgtc aaatcgaaaa cattgtggtt caaacgatcg
      721 agggcacccg gaactcgacg cggcggtgat cctcgccttc gacggcgtct atcgcgacga
      781 cggcatcttc gatcagcaga tggaacctgg cccggtcgat ggagtgcgct tgatgataca
      841 ccagaaagca tcacactaac gccggccagc gtgagcgtgc tgcattggag agccaaccaa
      901 taacgaggga cgcgtgaacc gtagagagat gctagcggcg ttaggtctgg tgacggcggg
      961 cggtgtcgtc gggtgcagcc cactagcggc gacagcggcc gcggggctca cgccgccgga
     1021 cccgcggaag atcttcgacg tcaaagcgta cggcgcaacc ggtgacggca agacgctcga
     1081 cacgccggcg atcaatcgcg cgatcgatgc atgttcctcc ggcggcggcg gaatggtgta
     1141 cgtcccgccc ggcacttacc tgtgcggcac gattcagctc aagtccaacg tcacgttgtt
     1201 cctcgaggcg gctgcgatca ttcaaggcag ctcgcggtcc gaagattttg gcctgatgac
     1261 cggcccgccg gaacaggggg acatgaacca gcggcatgtc atctttgccc gagatgccag
     1321 caacatcacg ctcgccggca ccggcacgat cgacggaaat ggcgccgcct attggacgca
     1381 gcgcgccagg cccgcgaccg atccctgggg agacgttgcc acttatgatt acgtcgcgtc
     1441 gcgccgccgg ccttcgccgc tgctggagtt ttacaactgc accgatctgg gaattcgcgg
     1501 cgtccacatc cgcaacgccc ccggctggac gcttcggccg atccagtgca gcaacgtctt
     1561 catcgacggc atcagcatca agaacccgat ctacggcctg aacaccgacg gcatcgacgt
     1621 cacctgctgc aagaacgtct tcatctccaa ttgcctgatc gaaaccggcg acgacacgat
     1681 ctgcctcaaa agcgaaagcc cctacggcgg tgagccggcc gtcactaaaa acgtcacgat
     1741 caccaactgc gtgctgagct gctgctgcaa cggcttgaag atcggcaccg ccacgcacgg
     1801 cggattcgaa aacatcgtct tctccaactc ggtcatcttc aacgaagacg tgccgatcaa
     1861 tcagcgcgtg atcggcggcg tgtcgctgga agtcgttgac ggcggttaca tcgacggcgt
     1921 gctggtctcc aacatccgca tgcaacgcgt gcgagcgccg atcttcatcc gccggggtgc
     1981 gcgaaaggga cccaccgata acaaggtgaa ctatctccgc gggatcatga tcgagaacgt
     2041 gcacgcgacc ggcgcgatca tgaccagttc ggtgacggga ttgccggggg cgaatgtcga
     2101 ggatgtcacg ctg
//
LOCUS       DATIMH010000414         4169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2116895, whole genome shotgun sequence.
ACCESSION   DATIMH010000414 DATIMH010000000
VERSION     DATIMH010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116895"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2189
                     /locus_tag="VLI90_07100"
     CDS             <1..2189
                     /locus_tag="VLI90_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HSV14010.1"
                     /translation="TGNLTGITVTSPGSGYTSQPSVTLTGGGGTPVSPTASIAANAAS
                     GPMTFTGSGVTTIAPAAGSATSTGLNNVVVAGGTLKINAAPTPVAAYTFDNIGTQQNP
                     NYDLSNHGSGQSMDGSVSGSVDFSLAGHNGSGNAATFGGDGSSININSGITDLSGTSR
                     WSVAYWVKAASSGAGGAIFSKNTGGSWAAGNSVFYLTDTSATGGTSGSVPGAVRNNAQ
                     WIKGSTSVTDDSWHFVVMNDDGGTRSVYVDGAVSAISQDTFTGADAGTSVQLGTSTDT
                     NSADGAFPLLGSLDSVKFYNQSLTGTQILELMNTDTVGSVPTPSLPVTAAVNISTSGA
                     TLDVGASATIGSLSGVSGGSVVLEAGTFSVGDSTSTAFAGAISGAGSLLKQGSGTLTL
                     SGSNTYGGNTTVNAGTLAVTASGSLPSTTNLAASGGAVVLGNASQTVASLSGSTGGSL
                     TLNGTALTVSNGGTFAADWNAGSSPSSITVSGGTMAFGGATASNGTVNANAAATFAGT
                     TGSTAVTRSLAALNIGDGVTVGILHSAFPFTPTVLKPATTSFGAGSTIDISNNELVSN
                     GTAAAALLKLQSGQIFSSEPADTTKAIGYINLSGADAGKFEVRYTLKGDANLDGSVAV
                     GDLGALATSYGITTGMSWGNGDFNRDGKVDVGDLGALATNYGTHLATGPSAGDSAASA
                     AAVASPMALVASGGSAAVPEPASGALLGIAALGLIGGRRRRHRSLN"
     gene            2276..3283
                     /locus_tag="VLI90_07105"
     CDS             2276..3283
                     /locus_tag="VLI90_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV14011.1"
                     /translation="MKANNNRGRLRIRPAPARAFTLVELLVVIGIIGVLVAILLPALS
                     KARRQAQVLRCESNLHNIGIALINYAAVNRGYLPAFTGGGNWMWDLPAPTRNAIIRYG
                     ASRANFYCPTNDAQNHIALWNYAVLATDKNGNTLFNGNQNKPGATFADSTGNSFDSWP
                     MPEETGWSVLGYVFLIKRVVNDGPLAPSTTSASPNFPNGIVASPDTRNRHFDWQDRIV
                     PHNYAGKADLTGYVHPRISSQCEIAFDAIIANDVANPSSFGATFGGWSFNGSRIAHQS
                     NHWYGNSYNNGFPVGANYLYLDGHVEWRSISKANYNVLGGFNGRVITGTSAGFPIAFF
                     W"
     gene            3318..>4169
                     /locus_tag="VLI90_07110"
     CDS             3318..>4169
                     /locus_tag="VLI90_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HSV14012.1"
                     /translation="MKSHLVLLVMVFPCAASTAAAEGGFLFSTFRDDPTPASEQIYFG
                     ISPDGRHWDALAGGRPVLVSDVGEQGARDSFLLRAHDSQKVWLIATDLCIARNRSWHR
                     ATHSGSRSIVVWETDDLVHWSPARLVQVAPDDAGCAWAPEAIYDPETRDYLVYWASFT
                     GRDMFARNRIWAARTKDFRTFEKPFIYLERREHVVDIHIMRDGDTFYRFTKDDKVKTI
                     SMAKSQRLMGPWEDIPQFTAGQGNDSEGPICYRLPPTAGDGQPPEWCVLLDNLGARIG
                     PGKRCYEP"
BASE COUNT          754 a         1370 c         1365 g          680 t
ORIGIN      
        1 ccaccggcaa cctgactggc atcaccgtga ccagcccggg cagcggttac acgtcgcagc
       61 ccagcgtcac cctcaccggt ggcggtggca cgccggtgtc gcccaccgca tcgatcgctg
      121 ccaacgcggc cagcggcccg atgaccttca ccggttcggg cgtcacgacg atcgccccgg
      181 cggcgggcag cgcgacgtct accggtttga acaacgtcgt cgtcgccggc ggaacgttga
      241 agatcaatgc ggccccgacg ccggttgcgg catatacctt cgacaacatc ggcacgcagc
      301 agaacccgaa ctacgacctt tcgaaccacg gcagcggcca gtcgatggac ggcagtgtga
      361 gtgggtcggt tgacttctcg ttggccggtc acaacggcag cggcaacgcc gcgacgttcg
      421 gcggcgacgg ttcgtcgatc aacatcaact cgggcatcac ggacctcagc ggcaccagcc
      481 gctggagcgt ggcctactgg gtgaaggccg cttcgtccgg cgcgggtggg gcgatcttct
      541 cgaagaacac cggcggtagt tgggcggcgg gcaactcggt cttttacctg accgacacca
      601 gcgcgaccgg cggcaccagc ggttcggtgc ccggcgccgt ccgaaacaac gcgcagtgga
      661 tcaagggcag caccagcgtc accgacgata gctggcactt cgtcgtgatg aacgacgacg
      721 gcggaacgcg gtcggtctac gtggatggcg cggtcagcgc gatttcgcag gacacgttca
      781 ccggcgctga cgccggcacc tccgtgcagc tgggcacgtc caccgacacg aattctgccg
      841 acggcgcgtt cccgctgctt ggctcgctcg actcggtgaa gttctacaac cagtcgctga
      901 cgggcacgca gatcctggag ttgatgaaca cggacacggt cggctcggtg ccgacgccgt
      961 cgttgccggt gacggccgcg gtgaacatca gcacgagcgg ggcgacgctg gacgtcggcg
     1021 cgtcggcgac gatcggttcg ctgagcggtg tgtccggcgg cagcgtggtg ctcgaagccg
     1081 gcacgttcag cgtcggcgac agcacctcga cagccttcgc cggcgcgatc agcggcgcgg
     1141 gctcgttgct gaagcaaggc agcggcacgc tgacgctcag cggcagcaat acctacggcg
     1201 ggaacacgac cgtgaacgcc ggcacgcttg cggttaccgc cagcggcagc ctgccgtcaa
     1261 cgacgaacct ggcggccagc ggcggcgcgg tcgtgctggg caacgcgagc cagacggtcg
     1321 cgtcgctcag cggatcgacc ggtggatcgc tgacgctcaa cggcaccgcg ctgaccgtca
     1381 gcaatggcgg gacgttcgcc gccgattgga atgccgggtc gtcgccgtcc agcatcaccg
     1441 tctcgggtgg aacgatggcg ttcggcgggg cgacggccag caacggcacc gtcaacgcta
     1501 atgccgcggc caccttcgcc ggcacaaccg gatcaacggc ggtgactcgt tcactggcgg
     1561 cgctgaacat cggtgacggc gtgacggtcg gcatcctgca ctcggcgttc ccgttcacgc
     1621 ccaccgtcct caaaccggcg accacgtcct tcggcgccgg gtcgacgatc gacatcagca
     1681 acaatgaact ggtgtccaac ggcaccgcgg cggcggcttt gctgaagctg cagagcgggc
     1741 agatcttcag cagcgaaccg gcggatacga ccaaggcgat cggctacatc aacctgagcg
     1801 gcgccgacgc cggcaagttc gaggtgcgct atacgctgaa gggcgacgcg aacctcgacg
     1861 ggtccgtggc cgtcggtgac ctgggcgcgc tggcgaccag ctacggcatc acgaccggca
     1921 tgagctgggg caacggtgac ttcaaccgcg acggcaaggt ggacgtcggc gacctgggcg
     1981 cgctggcgac gaattacggc acgcacctgg caacggggcc gtcggccggt gatagcgcgg
     2041 cgtcggcggc ggccgtcgct tcgccaatgg cgctggtcgc atccggtggc agcgcggctg
     2101 tgccggaacc ggcttcgggc gcattgctcg gcatcgccgc tcttggactg atcggtggcc
     2161 ggcgtcgtcg ccaccgcagc ctgaactgat tttcctccgc tcgcggcggc ccgtacctga
     2221 ccggcgggcc gccgcacttg gaggatcgct gtgcgaacat tggaaggatt ccacgatgaa
     2281 agcaaacaac aatcgaggtc gccttcgcat tcggccggcg ccggcgcggg cgttcacgct
     2341 ggtggaactg ctggtggtga tcgggatcat cggcgtgttg gtcgcgatcc tgctgccggc
     2401 gctgagcaag gcccgccggc aggcgcaggt cctgcggtgc gagtcgaacc tgcacaacat
     2461 cgggattgcg ctgatcaact acgcggcggt gaaccgcggt tatctgccgg cgttcaccgg
     2521 tggcggaaat tggatgtggg accttcccgc gccgacgcgc aatgcgatca tccgctacgg
     2581 cgcgtcgcgc gccaatttct attgcccgac caatgacgcg cagaaccata tcgcactgtg
     2641 gaattacgcc gtgctcgcca ccgacaagaa cggcaacacg ttgttcaatg ggaaccagaa
     2701 caagcccggc gccacgtttg ccgacagcac cggcaacagc ttcgacagct ggccgatgcc
     2761 ggaggaaacc gggtggagcg tgctgggcta cgtcttcctg atcaagcggg tggtgaacga
     2821 tggaccgctg gcgcccagca ctacttccgc ctcgccgaat tttccgaacg ggatcgtcgc
     2881 cagccccgac acccgcaaca ggcattttga ctggcaggac cgcatcgtgc cgcacaacta
     2941 tgcgggtaag gcggatctca ccgggtacgt gcatccccgg atatccagcc aatgcgaaat
     3001 cgccttcgac gcgatcattg ccaacgatgt ggccaatccc agcagcttcg gcgcgacgtt
     3061 cggtggatgg tccttcaacg gctcacgcat tgcccaccag tcaaaccact ggtacggcaa
     3121 ctcgtataac aacgggtttc cggtcggcgc aaactacctt tatctcgacg ggcacgtcga
     3181 atggcggtcg atcagcaagg cgaactacaa cgtattaggc ggctttaacg gccgggtgat
     3241 caccgggact tccgccggat tcccgatcgc gttcttctgg taactccgcc cctgcgacca
     3301 ttgcgacagt tcaagaaatg aaaagccatc tcgttctgct ggtgatggtc ttcccttgcg
     3361 ccgcatcaac cgcggcggcg gagggtgggt ttcttttctc gacattccgc gacgatccga
     3421 cgccggcgtc agaacaaatc tacttcggca tcagccccga cggccggcac tgggacgcgc
     3481 tggccggtgg caggcccgtg ctggtcagcg atgtcggcga gcagggcgcg cgagattcgt
     3541 ttctgctgcg ggcccacgac agccagaagg tttggctgat cgcaacagat ctgtgcatcg
     3601 cgcgcaaccg cagctggcat cgcgccacgc actcgggcag ccgctcgatc gtcgtctggg
     3661 aaacggacga tctggtccac tggtcgcccg cgcgattggt gcaggtcgcg cccgacgacg
     3721 ccggttgtgc ctgggcgccg gaggcgatct acgacccgga aacgcgcgat tacctggtgt
     3781 attgggcctc ctttaccggg cgcgacatgt tcgccaggaa ccgtatttgg gcggcccgca
     3841 caaaagactt ccgcaccttc gaaaagccgt tcatctatct cgaacgccgc gagcacgtcg
     3901 tcgatatcca catcatgcgc gacggcgaca ccttctatcg cttcaccaag gatgacaagg
     3961 tcaagaccat cagcatggcc aagagccagc ggctgatggg cccgtgggag gacatcccgc
     4021 agttcaccgc cggacaaggc aatgactccg aagggccgat ctgctaccgc ctcccaccaa
     4081 ccgccggtga cggccagccg ccggagtggt gcgtgctgct ggacaacctc ggcgctcgca
     4141 tcggcccggg gaagcgctgt tacgagccg
//
LOCUS       DATIMH010000415         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5584431, whole genome shotgun sequence.
ACCESSION   DATIMH010000415 DATIMH010000000
VERSION     DATIMH010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5584431"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..539)
                     /locus_tag="VLI90_07115"
     CDS             complement(27..539)
                     /locus_tag="VLI90_07115"
                     /inference="COORDINATES: protein motif:HMM:NF045136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HSV14013.1"
                     /translation="MPLFTKFRDGSFRAFNATVVGDVTAGAESSFWFNAVVRGDVAAV
                     RIGRRTNVQDGAVIHCDTGVPNEIGDDVVIGHGAIVHGQAVGDGSLIGMGATLLSRTI
                     VGSECLIAAGAVVPPDLIVPDRMVVMGVPGKIARPVKEKELEYMRWLRGHYVELAQQY
                     LDGKFDVGEE"
     gene            713..>2905
                     /locus_tag="VLI90_07120"
     CDS             713..>2905
                     /locus_tag="VLI90_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14014.1"
                     /translation="MSLTFDSKLSAARASAIEALERRVLLSTYYVSPSGSDTNPGTLA
                     APWQTIAKVNDTVFSPGDQILLARGGQWRESLNASADGAPGAPIVYGNYGDANLPKPL
                     LIGSDILLTSRFTLLSGTTYTLQSTTPINWIYDNHAFTHNSEVFLRTSGSGDPSAEAD
                     NVNYVEQNAESFYYDASSATLYLNVGSSPAAHLITAAVREDAVYNNRHSNIHFVGLAA
                     TETAHDGGGYAFRSQLGDNIQIIDCDASLAGKHEFGAIDSNNFLGENLTAQLAAPDLG
                     YGGSSAYVSYSDSVNGRANDSAQWINLTWSNPGGAYPIFISHGDPGAIGSLFLQNLVS
                     LGGYGTGIIIYSTGPQERIVLLGGHLDGGTIQLDPDYSVVDGLTLTGDGADLFLGGSN
                     DVVQNSRLIGLAPNPFIGRNGGIIDGGFNNTIRFNTFHWNDEPGPGVSITHNNSNTHI
                     YANLFDVNQPIRLLYNTGGSLLDSDYNLFGTSYDTAPVFGVGDVNTPGKYTLAQWHAN
                     GYDLDSVVGDPKFNDAADGDLTLQSTSPALDLYTQTPDATPTSANHDLKGDTRPLGPA
                     YDAGAFEAPGPPLVLNGTTGPDAFYIRVDAANPAAVDVWQNTGHDGSGLPTFISPFDA
                     FYSIIVNGGGGGDGGDLLTLDFSNGPFGAPGITYNAGDGSGSGAGGNDAGGAIVAGGA
                     DSISVIGTTGSDNITVDSAQVQVNSITITLAQPTVVNVDGKGGSDTLTA"
BASE COUNT          546 a         1071 c          799 g          489 t
ORIGIN      
        1 ccctcctccc gcctcccccg ctctccctac tcctcgccca catcaaactt cccatccaga
       61 tactgctgcg ccagctccac gtaatgcccg cggagccagc gcatgtactc cagctccttc
      121 tccttgaccg gccgggcgat cttgccgggc acgcccatca ccaccatgcg gtccggcacg
      181 atcaggtccg gcggcaccac cgcaccggcg gcgatcaggc attcgcttcc gacgattgtc
      241 cggctcagca gcgtcgcccc catgccgatc aacgacccgt cccccaccgc ctgcccgtga
      301 acgatcgccc cgtggccgat caccacgtcg tcgccgatct cgttcggcac gccggtgtcg
      361 cagtgaatga cggcgccatc ctgcacgttg gtccgccggc cgatgcgcac ggccgccacg
      421 tcaccgcgga cgacggcgtt gaaccagaag ctggattccg ccccggcggt gacgtcgccg
      481 accaccgtgg cgttgaaggc gcggaacgag ccgtcgcgga attttgtgaa caggggcatg
      541 ttggggaact taaccggcct cgatcggctt ttcgatgctc gcgccgggcg gcaaatcgat
      601 caaaatcagt aaagttccgg atgattctct gcaggttaat cggatagact tgctcgactt
      661 tgaggttgcc gcgacgtatg cccaccagat gttcaggggg agaccgccgg acgtgtcact
      721 gacttttgat tccaagctct cggccgcccg cgcgagcgcg atcgaagcgc tcgagcgccg
      781 cgttctgctt tcgacgtatt acgtcagccc cagcggcagc gacaccaatc ccggcaccct
      841 cgccgcgcca tggcagacga tcgccaaggt gaacgacacc gtcttctccc ccggcgatca
      901 aatcctgctc gcccgcggcg ggcaatggcg cgaaagcctc aacgccagcg ccgacggcgc
      961 gccgggcgcg ccgatcgtct acggcaacta tggcgatgcc aacctgccca agccactgct
     1021 gatcggcagc gatatcctgc tgacctcacg gttcacgctg ctgagcggca ccacctacac
     1081 gcttcaaagc acgacgccga ttaattggat ttacgataat cacgcattca cgcacaactc
     1141 cgaagtgttc cttcgcacca gcggcagcgg cgacccaagc gccgaagccg acaacgtcaa
     1201 ctacgtcgag cagaatgccg aatcgttcta ctatgatgcg tccagcgcga cactctatct
     1261 gaacgtcggc tcgtcccccg ctgctcacct catcaccgcc gccgtgcgcg aagatgccgt
     1321 ctacaacaac cgtcacagca acattcactt cgtcggcctg gcggcaaccg agacggcgca
     1381 cgacggcggc ggttacgcct ttcgttcaca gctgggcgac aacatccaga tcatcgactg
     1441 cgacgcctcg ctggccggca aacatgaatt cggcgcgatc gactcgaata actttctcgg
     1501 cgagaacctg accgcccagc tcgccgcgcc ggacctgggt tacggcggtt cctccgcgta
     1561 cgtcagctat agcgacagcg tcaacggccg cgccaatgac agcgcgcagt ggatcaacct
     1621 cacctggtca aaccccggcg gcgcgtatcc gatcttcatc tcgcacggcg atccgggcgc
     1681 catcggatcg ctcttcctgc aaaacctcgt cagcctcggt gggtatggca ccggcatcat
     1741 catctattcg accggcccgc aggaacgcat cgtcctcctc ggcggtcacc tcgatggcgg
     1801 aacgatccag ctcgatcccg actattccgt cgtcgatggc ctcaccctca ccggcgatgg
     1861 cgctgatctg ttcctcggcg gcagcaatga tgtcgtgcag aattcccggc tgatcggcct
     1921 ggcgcctaac ccgttcatcg gccgcaacgg cggcatcatc gacggcggct tcaacaacac
     1981 gatccgcttc aacaccttcc actggaacga cgaacccggg cccggtgtgt cgatcaccca
     2041 caacaacagc aacacccata tctacgccaa tctcttcgac gtcaaccagc ccattcgcct
     2101 gctctacaac accggcggct cgttgctcga cagcgactac aacctcttcg gcaccagcta
     2161 cgacaccgcc cctgtcttcg gcgtgggtga cgtcaacacc cccggcaagt acacgctggc
     2221 gcagtggcac gcgaacggat acgatctcga ctccgtcgtc ggcgatccga aattcaacga
     2281 cgccgccgac ggcgatctca cgctgcaaag cacctcgccc gcgctcgatc tttacaccca
     2341 gacgcccgat gcgacgccga cctccgccaa ccacgatctc aaaggcgaca cccgcccgct
     2401 cggccccgcg tacgacgccg gcgcgttcga agcgccgggg ccgccgctgg tgttgaacgg
     2461 caccaccggg cccgacgcgt tctacatccg cgtggacgcc gccaaccccg ccgcggtgga
     2521 tgtgtggcag aacaccggcc acgacggcag cggcttgccg accttcatct cgcccttcga
     2581 tgcgttctat tcgatcatcg tcaacggtgg cggtggtggc gatggcggcg atctgctcac
     2641 gctcgacttc tccaacggcc ccttcggcgc gcccggcatc acttacaacg ccggcgacgg
     2701 cagcggcagc ggcgccggcg gcaacgacgc cggcggcgcc atcgtcgcgg gcggcgccga
     2761 ttcgatcagc gtcatcggca ccaccggtag cgataacatc acggtcgatt cggcgcaagt
     2821 gcaggtgaac tcgatcacca tcacgctcgc gcagccgacg gttgtcaacg tcgatggcaa
     2881 aggcggatcg gacacgctga ccgcc
//
LOCUS       DATIMH010000416         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2405857, whole genome shotgun sequence.
ACCESSION   DATIMH010000416 DATIMH010000000
VERSION     DATIMH010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405857"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VLI90_07125"
     CDS             <1..198
                     /locus_tag="VLI90_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14015.1"
                     /translation="LPGPVMLLETPASPDVMQQLRAWHRIWLVCDPQVTDADAWLRGY
                     HINAGKSWPVVGSVVEMVVNP"
     gene            complement(188..820)
                     /locus_tag="VLI90_07130"
     CDS             complement(188..820)
                     /locus_tag="VLI90_07130"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSV14016.1"
                     /translation="MNDPRPPTIGLRLAVLPFLFVPWLLLYEWVVYLGPLRGSFETYL
                     PGELHWPIWQWTELLYLSPYVLVTIAPFLAPTRAALWRFVSAAVIAIVIGMLFFLLVP
                     ALSTPRPFHATNMLGRMMLVDRMLDRNNGSASLPSFHVVWAFLGASVFAQCQRRRVLC
                     AGCWAWATLVAASCVFTGMHSLIDVVAGFVLFLLVQRLVWRLASPRHIMD"
     gene            1003..1533
                     /gene="pyrE"
                     /locus_tag="VLI90_07135"
     CDS             1003..1533
                     /gene="pyrE"
                     /locus_tag="VLI90_07135"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSV14017.1"
                     /translation="MSTMTRDQLAQRIADVALLRGEFTLRSGRKSNFYLDKYRFETQP
                     DILIELGKMFAEHITSDVQRIAGPELGAVPLAAAAAMASGKPAIFVRNQKKAYGSNKQ
                     IEGVYNAGETIVIVEDIMTTGGQVVEAAKTLEAAGLKVKKIVGVIDRLEGARQNIEAA
                     GYVFESLFTTKDLGIA"
     gene            1534..2322
                     /locus_tag="VLI90_07140"
     CDS             1534..2322
                     /locus_tag="VLI90_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HSV14018.1"
                     /translation="MDAKLPEIIAALTGATPVQWDRLTWPQVQRLRDGGMDMCLLPLG
                     ATEQHGPHLPLNTDSVFATACCAYASAKTGVPVLPTISYGCSLGHTDRWPGTISLFPE
                     TLSLTVREIVGFLMRTGFKRLLIVNSHWGNTSSLRCAIDRLRFDHFGEFQIGLKNTFE
                     LTPGIWQQFIDDGADFHANRAETALMLYFDPSAVRVDQIQDDEDRTGGKVFTYVVPQT
                     SRNGVTGFPSRATVEDGRRLFVEMAEALARFVAAGMTELPPSLS"
BASE COUNT          503 a          690 c          817 g          419 t
ORIGIN      
        1 ctacccgggc cggtgatgct gctggaaacg ccggcgtctc cggacgtgat gcaacagctg
       61 cgcgcatggc atcgcatctg gctggtatgc gatccccaag tcaccgacgc cgacgcctgg
      121 ctgcggggtt atcacatcaa cgcgggcaag agttggccgg tcgtcggcag cgtcgtggag
      181 atggtggtca atccataatg tgtcgcggcg aagccaggcg ccagaccaag cgctgaacga
      241 gcaggaacag cacgaagccg gcgacgacgt cgatcagtga gtgcatcccg gtgaagacgc
      301 aactggccgc aacgagcgtt gcccacgccc agcaaccggc gcatagcact cgacgacgtt
      361 gacattgcgc gaacaccgac gcgccaagaa atgcccacac gacgtggaac gacggcagcg
      421 aagcgctgcc gttgttgcgg tccagcatgc ggtcgaccag catcatgcga ccgagcatgt
      481 tggtcgcatg gaatggtcgc ggcgtcgaca gcgcgggcac cagcagaaag aacagcatcc
      541 cgatgacgat ggcgatcacc gccgcggaga caaagcgcca aagcgcggcc cttgtgggcg
      601 cgaggaacgg cgcgattgtg acgagcacgt aagggctgag atacagcagc tcggtccatt
      661 gccagatcgg ccaatgaagt tcacccggca ggtacgtttc gaatgagccg cgcaacggcc
      721 cgagatagac gacccattcg tacagcagaa gccacgggac gaagagaaac ggaagcaccg
      781 ccaagcgaag cccgatcgtc ggcggtcgtg ggtcgttcaa caaacgcgca agccgatcgc
      841 gccgcggcac agcggcagca ttcgagaagc catgcctgct gtctggagaa tcaaccgtca
      901 ttgcacacca acaacagtta gcaaccgctg ttccaaccgc gcctgcggaa tatcggtagc
      961 ggtatttgac gagcctcggc ttcgcgcaga tattccgcgt ccatgtccac catgacgcga
     1021 gatcaactag cccaacgaat tgcggatgtc gccctgctgc gcggggagtt taccctgcgt
     1081 tcggggcgaa aaagcaactt ttacctcgac aaatatcgct tcgaaaccca accggacatt
     1141 ttgatcgagc tgggcaagat gttcgcggag cacatcactt ctgacgtgca gcgcatcgcc
     1201 ggtccggagt tgggcgcggt tccgctggcg gcggcggcgg cgatggcgtc gggcaagccc
     1261 gccatcttcg ttcgcaatca gaagaaggcg tacggctcca acaagcagat cgaaggcgtc
     1321 tacaacgccg gcgaaactat tgtgatcgta gaagatatca tgaccaccgg cggtcaagtc
     1381 gtcgaagccg ccaagacgct ggaagcggca ggcttgaagg tgaagaaaat cgtcggcgtg
     1441 atcgatcggc tggagggcgc gcggcagaac atcgaggccg ccgggtatgt cttcgaaagc
     1501 ttgttcacca caaaggattt gggcatcgcc tagatggacg cgaagttgcc ggagatcatc
     1561 gccgcgctca ccggggcaac gccggtgcaa tgggatcggc tgacgtggcc gcaggtgcag
     1621 cgcctgcgcg atggtgggat ggacatgtgc ctgctgccgc tgggggcgac cgagcagcat
     1681 gggccgcact tgccgctcaa taccgattcg gtgttcgcga ccgcgtgctg tgcgtacgcg
     1741 tcggcgaaga cgggcgtgcc ggtgttgccg acgatttctt acgggtgctc gctggggcac
     1801 accgaccggt ggccggggac gatttcactg tttccggaaa cgctgtcgct gacggtgcgc
     1861 gagatcgtcg ggtttctcat gcgcacgggg ttcaagcggc tgctgatcgt caactcgcat
     1921 tggggcaaca cgtcatcgct gcgctgcgcg atcgatcggt tgcgcttcga tcactttggc
     1981 gaatttcaga tcgggttgaa gaacacgttc gagctgacgc cggggatttg gcagcagttc
     2041 atcgatgatg gcgcggactt tcacgcgaac cgagcggaga cggcattgat gctgtacttc
     2101 gatccgagcg ccgtgcgcgt ggatcagatt caagacgacg aggaccgcac gggcgggaag
     2161 gtgttcacgt atgtggtgcc gcagacgagc cggaatgggg tgacggggtt tccgtcgcgc
     2221 gcgacggtgg aggacgggcg gcggttattt gttgagatgg ccgaagcgct ggcgcgcttt
     2281 gtggcggcgg ggatgacgga gcttcctccc agtttatcct gagcggagcg cgtatccgta
     2341 cgcagtcgaa ggatctggct ggcgcgtcga agaaccgttg attgcgaaaa cggcgcggca
     2401 gtcgcttgcg gtttcgcaga cccgggtgg
//
LOCUS       DATIMH010000417         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2984835, whole genome shotgun sequence.
ACCESSION   DATIMH010000417 DATIMH010000000
VERSION     DATIMH010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2984835"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="VLI90_07145"
     CDS             <1..1600
                     /locus_tag="VLI90_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14019.1"
                     /translation="IPASGGAVAVAGDALGRQFIAYFDTVDSDLKYFTRMPNGAVSPD
                     RVIDASPGAGAQLAIAVQHNLPVVAYYDSTAQQLKFARMINGVWHKTVVDQTGNVGQN
                     PSIAIDAAGNAAISYYDVTNGDLKVARQTGKTFSVTTIDSTGDVGLFSSIALNPATHL
                     LGIAYQDTTGGVLKLATEGTSGFTPAAIPGTIATGQAPSLAFDSAGNTFVSFFNSLAA
                     GGPAVDVLEFTNSAWATVEHFTLSGAGATAGSIETIFPTAATAALPLIVFTDTASHAL
                     LLGQHSTTTGDATSMLFNDAGPIAAASAGATNKLDLITQPTGAAYSLLLIQDATAIAP
                     AVPAGVFNATIPANAEISGAGNAGLPDATGGVAPIGFNIPAGVTRVQLSSVTGTISLN
                     SGAGTNDADGVVVTGTYPTTLANASQMGAFGGLSGITIPGAGALVGVFVGATVPSGSP
                     PADLDYTTLGTTAASSTPLLNQVFYIGDGKTADGSGAVQSFIIPSGATKLFLGISDAQ
                     NFAGAPGSYGDNSGNFAVTLNFGG"
     gene            1791..2384
                     /gene="aat"
                     /locus_tag="VLI90_07150"
     CDS             1791..2384
                     /gene="aat"
                     /locus_tag="VLI90_07150"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HSV14020.1"
                     /translation="MAEPRLDPETLLSAYAQGVFPMGDRDGVVRWYTADPRGVIPLDD
                     RFHVPHTLAQLARNPRRFEIRINHNFTDTMRACQQQRSDGTWINQKLITAYTRLHELG
                     FAHSVETWWDGELTGGLYGVSLGGAFFGESMFHRRRDASKLALIHLVDRLRERGYELL
                     DAQASTPHLRRFGCIEIPATEYLSRLDLALKKRCEFV"
     gene            2484..>2834
                     /locus_tag="VLI90_07155"
     CDS             2484..>2834
                     /locus_tag="VLI90_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HSV14021.1"
                     /translation="MVHDEVIPILDFGSQYAQLIARRVREAGVFSELVRPDISVDELK
                     RLSPKGIILSGGPSSVYEPNAPRCDPKIFDLGVPIFGICYGMQLGAQILGGQVKPAKA
                     REYGRAKLTVAADDA"
BASE COUNT          507 a          983 c          882 g          462 t
ORIGIN      
        1 aattcccgca tcgggcggcg cggtcgccgt cgccggcgat gcgctgggcc ggcagttcat
       61 cgcgtacttc gataccgtcg atagcgacct gaaatatttc acgaggatgc cgaacggcgc
      121 ggtctcgccc gatcgcgtga tcgacgccag ccccggcgca ggcgcgcagc tggccattgc
      181 ggtccagcac aacctgccgg tggttgcgta ttacgattcg accgcgcagc agctgaaatt
      241 cgcgcgcatg atcaacggcg tctggcacaa gacggtcgtc gatcaaaccg gcaacgtcgg
      301 gcagaacccg tcgatcgcca tcgacgccgc ggggaacgcg gcgatttctt actacgatgt
      361 caccaacggc gacctgaagg tggcgcgaca gacaggcaaa actttctcgg tcacgacgat
      421 cgattcgacc ggtgacgtcg gcctgttcag cagcattgcg ctgaaccccg cgacgcacct
      481 gctcggcatc gcgtaccagg acacgaccgg tggcgtgctg aagctggcga ccgaaggcac
      541 cagcggcttc acgcccgccg ccatccctgg aacgatcgcg accggacaag cgccgtcgct
      601 ggcgttcgat agcgcgggca acacgttcgt cagcttcttc aattccctcg ccgcgggtgg
      661 tccggccgtc gatgtgctgg agttcaccaa tagcgcctgg gcgacggtcg aacatttcac
      721 gctgtccggc gcgggcgcga cggccggcag catcgagacg atcttcccca cggccgcaac
      781 cgcggcattg ccactcatcg tcttcaccga caccgccagc catgcgctat tgctcgggca
      841 gcacagcacg acgaccggcg acgcgacttc catgctcttc aacgacgccg gcccgattgc
      901 cgcggcgtcg gcgggggcaa ccaacaagct ggacctgatc acgcagccga ccggagccgc
      961 ctattcgctg ctgctgatcc aggatgcgac cgccatcgcc ccggcggtgc cggcgggcgt
     1021 gttcaacgcg accattcccg ccaatgccga gatttccggc gccggcaacg ccggcctgcc
     1081 cgacgccaca ggtggcgtgg cgccgatcgg cttcaacatt cccgcgggtg tgacgcgagt
     1141 gcagctcagc tccgtcaccg gcacgatcag cctcaactcc ggcgcaggca ccaacgacgc
     1201 cgacggcgtc gtcgtcaccg gcacgtatcc gacgacgctc gccaacgcat cgcagatggg
     1261 agcgttcggc ggcctgtccg gcatcacgat ccccggtgcc ggcgcactgg tcggcgtgtt
     1321 cgtgggcgcg acggtgccaa gcggctcgcc gccggcggac ctggattaca ccacgctcgg
     1381 caccaccgcc gcgtcgtcta cgccgctgct gaaccaggtc ttctatatcg gcgacggcaa
     1441 aaccgccgac ggcagcggag cggtacagag cttcatcatc cccagtggcg ccacgaagct
     1501 gttcctcggc atctccgatg cgcagaactt cgccggagcg cccggctcgt acggggacaa
     1561 ctccgggaat tttgcggtga ccctgaactt tggcggatga ccccaatcgt ttagccgccg
     1621 cgacctcgcg gcgcggacgt agaaaaatgc cgcgaggccg cggcggctaa acgacagccc
     1681 ttgtggcgcg ctcggcgcgc tgtttaccaa tcccccctcg actacaatcc gccgtcaccg
     1741 gtggcgcgtc ccgcgtgata tctcccagac gcgcgccgcc gcgatcgacg atggccgaac
     1801 cgcgactaga ccccgagacg ctgctgtccg cgtacgccca gggcgtgttc ccgatgggcg
     1861 atcgcgacgg cgtcgtgcgc tggtacaccg ccgacccccg cggggtcatc ccgctggacg
     1921 atcgctttca cgtcccgcat acgcttgccc aactcgcgcg caatcccagg cgcttcgaga
     1981 tccgcattaa ccacaacttc accgacacga tgcgggcctg ccagcagcag cgcagcgatg
     2041 gcacgtggat caaccagaag ctgatcacgg cgtacacgcg attgcacgag ctgggctttg
     2101 cccacagcgt cgaaacctgg tgggacggcg aactgaccgg cggcctgtac ggcgtcagcc
     2161 tcggtggcgc gttcttcggc gaaagcatgt tccaccgccg ccgcgacgcg agcaaactgg
     2221 cgctgatcca cctggtcgat cgactgcgcg agcgcggcta cgaactgctc gacgcgcagg
     2281 catcaacccc gcacctgcgg cggttcggct gcatcgaaat ccccgcaacg gaatatctgt
     2341 cgcggctgga tctggcgctg aagaagcggt gtgagtttgt ctaataatcc gccgcttgcg
     2401 gcttcgcaga cccaacctgc ccgatctgcg aagccgcaag cggcggattg gcagttgaat
     2461 cgcttttccg gtatcaattc gcaatggtcc acgacgaagt cattccgatc ctcgatttcg
     2521 gttcgcaata cgcccagctg atcgcccgcc gcgtgcgcga agcgggggtc ttcagcgaac
     2581 tggttcgccc cgatatctcc gttgatgagc tgaaacgcct gagccccaag gggatcatcc
     2641 tctccggcgg gccgagcagc gtgtacgagc cgaacgcgcc gcggtgcgat ccaaagatct
     2701 tcgacttggg cgtgccgatc ttcggcatct gctatggcat gcagctgggc gcacagatcc
     2761 tcggcggaca ggtaaagccc gcgaaggcgc gcgagtatgg acgcgcgaag ctgaccgtgg
     2821 cggcggacga tgcg
//
LOCUS       DATIMH010000418         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2117180, whole genome shotgun sequence.
ACCESSION   DATIMH010000418 DATIMH010000000
VERSION     DATIMH010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2117180"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="VLI90_07160"
     CDS             complement(<1..1907)
                     /locus_tag="VLI90_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV14022.1"
                     /translation="MRVSLERAMRLARRSTLSEAEMSLVNGRNLKRARRSMVCSAAVG
                     AAWAGILCPGGATAQAASGTWITNATGLWSASTNWSGGVVADGSGNTADFSTLDISGT
                     SLTVNLDSARTIGQLLFEDQTNLSTDWILANNATPANVLTLGTGAGTPTISVFDRTAT
                     ITTILAGTQGLAKNGGGTLVLAAANTLTGGVSSSGGLLKLDFSQATSPTTNIIPASNA
                     FTLNGGTLQISGKASTTNSQAFAGTTLSGGGGLITMSPATTNPLLLNLGAITRSPGAA
                     LNVTLSGTSSATNGVASSTANNSTGIIGGYATVGGATNAGDWAANNGANVVAYTAYNT
                     AFPTTGGDSSINYSTTAGSTLTSDVIANSLKVISGANTYALGGNNLTFSGAAGGLLYA
                     DSSASSFTGTGVLGAGSGNEFIVQDWGAGALTIGTPIIGTSVGETGRLTKAGTGTIVL
                     TAANTYTGGTMLVGGQITVSNNAALGTGSIEINPNGVVGTGNGCKLLLSSGVTIANNI
                     IMDRATAGSGTNGTIDGSAGATGISGNITINGIANPGGTFIGPTTAGTFLTITGSITN
                     NLAADNGGTDAIIVRNGNVLLSGGGSYYRFDERAGTLAIGVSNGVATNASVDLGGNGT
                     ATFDLAGLNQTL"
     gene            complement(2053..3177)
                     /locus_tag="VLI90_07165"
     CDS             complement(2053..3177)
                     /locus_tag="VLI90_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSV14023.1"
                     /translation="MVAAGCMSIVRVANHAGVSTATVSRVLNNMPGVRAETIRQVRAA
                     VEALNYEPGQLRRIRRNGAASTTARPRRRTNAIAIITVGQSRHWLQLPVMAASVAGIS
                     RGARELGYRLLLEEALDVEQPLPAALGRQVDGAIVFLSSVLVGPAFYAALNAIARQIP
                     AVWVMGGGPGGVAVDHVAPHNHAVGHLAFEYLKSRGCRDVAFLSKLPAWPVMRDRGQA
                     FCSAAHDHAISCGTFLLSDDARDGALFGAGAIVEADLSTLVDRLVESKPRPTGLFVSA
                     DETTVNVYPLLRRAGLRPGEDIHIVSCDNEQIRLAGLSPRPASIDTGAEEIGWRAVHR
                     LMSRMENPAEPPVLINVAPRLVQGQVGTSNAGDDEAPPSD"
     gene            3368..4453
                     /locus_tag="VLI90_07170"
     CDS             3368..4453
                     /locus_tag="VLI90_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSV14024.1"
                     /translation="MSIIQVAKAAGVSTATVSRVLNDMPGVSDETVRQVRAAAEALRY
                     TPLRARRGNRPMPKPGGTASRSRPRTGNIAVITLGQTRDWLRLPIMAAVVTGIQRGAG
                     EFGLRLMLDDVPDPMRPSPLIQGKKIDGAIVFLPGGMPVGTYAPVLEKVRERIPIVWA
                     MGMEFSAADVDHVTPDNISTGFLAHSYLISKGCTQLAYLTANPEWPFMRLRGQAFLNL
                     ALDANQPASAFIVTSSPTLAESYGRRVVTAADLEQLVAALARSRPRPDGVFISNDATT
                     ASIYPLLARHGLEIGRDLTIVSCDNEDVRLSALHPRPASIDTGAEEIGFRAVIRLQSR
                     LQRPDDPPLVIQVAPHLVLPPTADAGH"
BASE COUNT          741 a         1471 c         1440 g          818 t
ORIGIN      
        1 agcgtttgat ttaaccccgc cagatcgaac gtggccgtgc cgttcccgcc aaggtccacc
       61 gaggcgttgg tcgcgacgcc attgctgacg ccgatcgcca gcgtgccggc gcgttcgtca
      121 aagcggtaat aactgccgcc gcccgacaac aacacgttgc cgttgcgaac gatgatcgcg
      181 tcggtgccgc cattgtcggc ggcaaggttg ttggtgatcg acccggtgat cgtgaggaat
      241 gtcccggcgg tggtcggccc gatgaacgtg ccgccagggt tggcgatgcc gttgatcgtg
      301 atgtttccgc tgatgccggt ggcgccggcg ctgccgtcga tcgtcccatt cgtgccgctg
      361 ccggccgtgg ctcgatccat gatgatgttg ttcgcgatgg tcaccccgct ggaaagcagc
      421 agcttgcatc cattgcccgt gccgaccacc ccgttgggat tgatctcgat gctgcccgtg
      481 cccagcgcgg cgttgttgct gacggtgatc tgtccaccaa cgagcatcgt gccgccggtg
      541 tacgtgttcg ccgccgtcag cacgatcgtg cccgttcccg ccttggtcaa ccggccagtt
      601 tcaccgacgc tggtgccaat gatcggcgtg ccgatcgtca gcgcgccagc gccccagtcc
      661 tggacgatga actcgttccc gctgcccgcg cccagcaccc ccgtgccggt gaacgaactg
      721 gcgctggaat cggcgtagag caacccacca gcagcgccgc tgaaggtgag gttgttgccg
      781 cccagggcat acgtgttggc gccggaaatg accttcagcg agttggcgat gacgtcgctg
      841 gtcagcgtcg agccggccgt ggtcgaatag ttaatcgatg aatcgccgcc ggtcgtggga
      901 aacgcggtgt tgtacgcggt gtatgcgacc acgttggcgc cgttgttggc ggcccagtca
      961 ccggcgttgg tggcgccacc gacggtggca taaccaccga tgatgcccgt ggaattgttc
     1021 gccgtgctgc tggccacgcc gttcgttgcc gacgacgtgc cgctgagcgt gacgttcagc
     1081 gctgcgccgg gactgcgcgt gatcgcgccg aggttcaaca gcaacgggtt agtggtcgcc
     1141 ggcgacatcg tgatcaaccc accgccgccg ctcagggtcg tgccggcaaa cgcctggctg
     1201 ttggtggtgc tcgccttgcc gctgatctgc agcgttccgc cgttcagcgt gaacgcgttc
     1261 gacgcgggaa tgatattcgt ggttggggaa gtcgcctgcg aaaaatcgag cttcaacaag
     1321 ccgccactgc tgctcacgcc gcccgttaag gtgttggccg cggccagcac gagcgtgccg
     1381 ccgccgttct tcgccaggcc ctgcgtgccg gccaaaatgg tcgtgatcgt tgcggtgcga
     1441 tcgaagacgg aaatcgtcgg tgtgcccgcg ccggtgccga gcgtcagcac gttggccggc
     1501 gtggcgttat tggccaggat ccagtcggtg ctcagattag tctgatcttc gaacagcagt
     1561 tggccgatcg ttcgcgcgct gtcgaggttc accgtcaggc tcgtgcctga gatgtccagc
     1621 gtgctgaagt cggcggtgtt gccggacccg tcggcgacga cgccgccgct ccagttggtt
     1681 gacgcgctcc ataaaccagt cgcgttggtg atccacgttc cgcttgccgc ctgcgcagtg
     1741 gcgccgcccg ggcaaaggat gccagcccat gccgcgccta ccgcagccga gcaaaccatc
     1801 gatcgacgcg cacgcttgag attccgaccg ttgaccagcg acatctccgc ctccgaaaga
     1861 gttgatcggc gcgcgaggcg catcgcccgc tcaagcgaca cgcgcatcgc agccgtttcc
     1921 gttcgtcgtt ccggacaccc gtccgcaacg cagcagcatt cacatgcact tgcaacagat
     1981 ttgatctgac ttccgcgctc gcgcgttcgc cgcagccgca gccgcggcgg atgaacggcg
     2041 ggctttgctt tttcagtcag acgggggcgc ctcgtcatcg cccgcgtttg acgttccaac
     2101 ttgcccttgc accagccgcg gggcgacgtt gatcagcacc ggcggctcgg cagggttttc
     2161 cattcggctc atcaggcgat gcaccgcgcg ccagccgatt tcctcggcgc cggtgtcgat
     2221 cgacgccggc cgcggggaaa gcccggccag gcggatctgt tcgttgtcac aggagacgat
     2281 gtggatgtcc tcgccggggc gcaggcccgc gcggcgaagc agcggataga cattgaccgt
     2341 cgtttcgtca gcgctgacga acaggccggt cggccttggc ttggattcaa ccaggcgatc
     2401 gacgagcgtg gacagatccg cttcgacgat cgcgccagcg ccgaacagcg cgccgtcgcg
     2461 ggcgtcgtcg ctgagcagga acgttccgca gctgatcgcg tgatcgtggg cggcgctgca
     2521 gaaagcctgc ccgcgatcgc gcatcaccgg ccatgccggt agcttcgaga ggaacgcgac
     2581 gtcgcggcaa ccgcggctct tgagatattc gaatgcgagg tgacccaccg cgtggttatg
     2641 cggcgcgacg tgatcgacgg cgacgccacc gggtccaccg cccatgaccc ataccgcggg
     2701 gatctggcgg gcgatggcgt tgagggcggc gtagaacgcg gggccgacca ggacgctgga
     2761 gagaaacacg atggcgccat ccacctgccg gccaagcgcc gcgggcagcg gttgttccac
     2821 atcgagcgct tcttccagca gcaggcggta acccaattcg cgcgcccccc ggctgatgcc
     2881 ggcaaccgac gcggccatca ccggcagttg cagccagtgc cggctctgac cgacggtgat
     2941 gatcgcgatg gcgttggtcc gccggcgcgg ccgggcggtg gtcgaagcgg cgccgttccg
     3001 ccggatgcgg cgaagttggc ccggttcata gttcagcgct tcaacggctg cgcggacttg
     3061 acggatcgtt tccgcgcgga cgcccggcat gttgttcagc acgcgcgaca cggtcgcggt
     3121 tgaaacacca gcgtgatttg ctacgcgtac aattgacatg cagcccgccg caaccatcgc
     3181 cggttgagat gccgatgttg cgaatgcaat tgtaatatcc gtcgctgatt tagcaatgta
     3241 aaaatgtttt acttacagaa tgaaatcgct gtaacagttg aggagagccc cgaacccagc
     3301 cctaagcggc gagagatttt gaagttgcgg agcgggaatg gtatcttcaa gttgctgaag
     3361 gagggcaatg tcgatcattc aagtggccaa ggcggcaggc gtttccacgg cgacggtgtc
     3421 gcgcgtgctg aacgacatgc cgggcgtgtc ggacgagacg gtccggcagg tccgagccgc
     3481 cgccgaagcg cttcgttaca ccccccttcg cgcccgccgg ggcaatcgac cgatgcccaa
     3541 gcccggcggc accgccagcc gcagccggcc gcgcaccggc aacatcgccg ttatcacgct
     3601 cggccagacc cgcgattggc tacgcctgcc catcatggca gccgtcgtca ccggcatcca
     3661 acgcggcgcc ggcgagttcg gccttcgcct catgctcgat gacgtcccgg accccatgcg
     3721 gccaagcccg ctcatccagg gaaagaagat cgacggcgcg atcgttttcc tgcccggggg
     3781 catgcccgtc ggcacctatg ccccggtcct ggaaaaggtg cgggaacgca ttccaatcgt
     3841 ctgggcgatg ggcatggaat tctcggccgc cgatgtcgat cacgtcacgc cggataacat
     3901 cagcacgggg tttttggccc attcctacct gatttccaag ggatgcacgc agttggcgta
     3961 tttgacggcc aaccccgagt ggcccttcat gcggctgcgc ggtcaggcgt ttttgaacct
     4021 cgccctcgac gccaatcaac ccgccagcgc gttcatcgtg accagcagcc cgacgctggc
     4081 cgagtcgtac ggccggcggg tcgtcacggc ggctgatctg gagcagctgg tcgccgcact
     4141 ggcgcgctcc cggccgcgcc ccgatggggt tttcatttcc aacgacgcca cgacggcttc
     4201 aatctaccca ctccttgcca ggcacggctt ggaaatcggc cgcgatctca cgatcgtctc
     4261 gtgcgacaac gaagacgtgc gtctgtcggc gttacacccc cgcccggcga gcatcgacac
     4321 cggggcggaa gagatcgggt tccgggctgt catccgcctg cagtcccgcc ttcagcgacc
     4381 ggacgacccg ccgctggtca tccaagtcgc gccacacttg gtccttcccc ccaccgccga
     4441 cgccggtcac tgatatcccc gacaaatgta
//
LOCUS       DATIMH010000419         9440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5008594, whole genome shotgun sequence.
ACCESSION   DATIMH010000419 DATIMH010000000
VERSION     DATIMH010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5008594"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3813
                     /locus_tag="VLI90_07175"
     CDS             <1..3813
                     /locus_tag="VLI90_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14025.1"
                     /translation="ADEFTNITFKVDIADAGGASDTKNTLISVADTPPTADLSTSANA
                     SNEGAVYTLHVGATTDPGPNHITGYAINWGDGNVDTFASGGDPANANFTHTYTDGPAA
                     RHISMTLIDEDGPHANSGTHDVTVNNVNPTATLQNLFPSISEGDAGSLNFTAQHDDSS
                     VDTASGFHYAYDFNNDGIFDSGDGSYGGSGTSSSINVPASYLIIPGQHSVKARIIDKD
                     GGFTDYTTTITSANVSPTVNLPANLTAGAGLPFTFTGSFSDPGTSDGPWTGVVDYGDG
                     SGFVALSVNSANHTFQLSHTWATSGPYTLVVHISDHPVSPDGIQPPAASLTGAASSAV
                     NVTATTFQVINFTPTASGFDVQFNRGADLSKLNLYGSSFGGFGASDVSFVGNNTGVVA
                     GSLIWDPSTDTAHFVATGGRLAADTYNVTLFSRADGWTDTSNNALDGDGNNTNGGDFA
                     RQFVLSYPLPPRTVSMPDFARGPGQTVDLPNTSSGAGIPITISDAANVLSVDFDLVYN
                     TSLLNITGATLASGLPADWGLTINFISPGRIKFTASGTTPLSAGAKQIIKLTATVPQT
                     AAYAGAADLQIQNLSVNEGNIASVGDSAVEKVAYFGDASGNAVYTGLDAGLISRVVVH
                     LDSGFDAYPLVDPVIVGDVTGDGSLSGLDASFVAQKASNLPRPEIPNIPTGFSIVGGG
                     IDPTFTGSSHTLGLRGGQADFTVLLDQITGITDIGFTITASYTAGVLHLANGDISLGE
                     ALPAGWSVVANTDDINGNTVLTFFNGGTNMTSDATKPIVHLGFHVPGNDNPGVTPVQL
                     SGPSVDGTPGHQVSYSYVDGSIDIAPFFTGTAGDDQYTVRLNGTGDTVQVWENQPTSG
                     APTYSFAASLLSLANFNLFKFTTLGGNDTLTIDYSNGNPLPAGGVIFDGGTNTAGGDT
                     LVLKLGDGGNAVTVDGSGVKVGSTPLNLTSDEHVVINGGSGDDSVTQTAVPAAPFAFN
                     GGAGNDSLSIMAGSYTFTTDASATTASLTVNVDGAGSSVTFSSTEHLAGLHLTGSGTA
                     KITSTATPFTPSVLNVHSLSIASGSTLDITNNEAIIDSSLASIQPDLQAGRIFSSTPV
                     AGEGLGSHALTPTQSVVRLSLLGDTNLDGSVDVGDLGQLASNYGQTSGATWEDGDTNN
                     DGAVDVADLGMLATNYGVSLSNGAAAPSSQAAVFASANVVTAPVATPAAAVAQPAALL
                     STTSTTISTAKSDVPAVTVTTKSTAKKADHSQVSGNHTRVEPSSTLPLKHKGQKKGDI
                     LGDDLKSL"
     gene            4157..4321
                     /gene="csrA"
                     /locus_tag="VLI90_07180"
                     /pseudo
     CDS             4157..4321
                     /gene="csrA"
                     /locus_tag="VLI90_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
     gene            4379..4870
                     /locus_tag="VLI90_07185"
     CDS             4379..4870
                     /locus_tag="VLI90_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HSV14026.1"
                     /translation="MRAGNGPAPTGDTPEARLLLRAALDQARHSAAGGGLPIGAVLVR
                     NDQIIGRGCDRSIQSGDPTATAVIDCLRNAGRQLAYGDTVLYTTLLPDHVAAGAVVQL
                     GIGRVVIGEAVHARDGAPPKPPVANLLRAAHVEVLDLHDSACVEMVDTFMREHPERWR
                     SSY"
     gene            5010..5498
                     /locus_tag="VLI90_07190"
     CDS             5010..5498
                     /locus_tag="VLI90_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14027.1"
                     /translation="MAGNAPNISEVRAAIDLYLGHAFAGDADLPHAVAAIVQQIRDAD
                     DAEVLSLPMWERDGAQPACRYCLRLGNRYYPHMKLVLERRPDGLGYVLRVDGHDQHVL
                     ATPVGAACEAFYEVVRHDAEIARDIEAAWEQHGLNTLRGFIASHAQTTQRFSPADVVK
                     AT"
     gene            complement(5515..6057)
                     /locus_tag="VLI90_07195"
     CDS             complement(5515..6057)
                     /locus_tag="VLI90_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV14028.1"
                     /translation="MRRLRRLCARRRRRRRGYRAIVIDSELVISPLRSVADGDAVVAL
                     FVAQMVDHQIVTDPATIRRVVRMITDEPRVGFILIAWHGATPVGVAYASAIISIEHGG
                     ASGWLEELYVLPQARRQGVGTQLLNEVIAEATRRGWRALDLEVEAGHDAAARIYHARG
                     FKPRTRQHWYFVISPIDPNQ"
     gene            complement(6079..6525)
                     /locus_tag="VLI90_07200"
     CDS             complement(6079..6525)
                     /locus_tag="VLI90_07200"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSV14029.1"
                     /translation="MPSNTIRLHRVLRATPERVYRAFLDPAAMAKWLPPHGFTGTVHQ
                     MDAKVGGKWRMSFTNFSSGGGHSFGGEYLELIPHERIRYTSRFDDASLAGEMQTTITL
                     KKVFCGTELNAVQEGIPAVIPAEACYLGWQESLTLLAQLVEPEIPG"
     gene            complement(6713..7510)
                     /gene="sdhB"
                     /locus_tag="VLI90_07205"
     CDS             complement(6713..7510)
                     /gene="sdhB"
                     /locus_tag="VLI90_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016508523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HSV14030.1"
                     /translation="MNPTVTDATPPRAADAAGGKAVIIRIRRQDGPGKPNRWEEFSVP
                     YRPNLNIIACLQWIAAHPVTTDNVQTTPPVWDSGCLEEVCGACTMLINGKTRQSCSAL
                     IDKLDQPITLEPMSKFPLVRDLFVDRQRLFNDLKRVKAWVPIDGTYDLGPGPAVSQAR
                     QEERYPLSRCISCGCCLEACPQYTPSNHFVGAAVISQAKLFNDHPTGAALKEERLETL
                     MEEGGVADCGKAGNCVEVCPKEIPLLESIAGIQRQATVYTIKKFFSR"
     gene            7946..>9440
                     /locus_tag="VLI90_07210"
     CDS             7946..>9440
                     /locus_tag="VLI90_07210"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HSV14031.1"
                     /translation="MADTVGLAQLDLASVHQDFGKPGVNKSIDGHPITIGGKSFEHGL
                     GTHAQSVWWINVNGRGERFTASVGLDDEVKSDPKSSRVGVEFKVIGDRKTLFRSGRMK
                     VGEAPKPVDVDLDGVKMLILVVSPINGNIDYAHCDWADGTITYSGERPVALEPPPDMP
                     VVLTPPAPATPRINGAKVFGVRPAHPVLYMIAATGDRPMTFNAENLPPGLSLDQSSGL
                     ITGSTEKAGDNAVTIKATNAKGSADAQLHIMVGDRIALTPPMGWNSWNCFATAVSDEK
                     VRAAADAMVNAGLVNHGWTYINIDDCWEVKASEPPENRRDASGRIKTNNKFPDMKALA
                     DYIHSRGLRAGLYSSPGPSTCGGYTASYRYEEQDAREYADWGFDYLKYDWCSYGNIAD
                     QIKKAPNPPAQLEIFQHPYNVMHEALAKQNRDILFSLCQYGMGDVWEWGDQVGGNCWR
                     TTGDIRDTWGSMAGIGFKQAGHEKFAGPGHWNDPDMLVVGKVGWGPAL"
BASE COUNT         1735 a         3015 c         3054 g         1636 t
ORIGIN      
        1 gccgatgagt tcaccaacat caccttcaag gtggacatcg ccgacgccgg tggcgcgagc
       61 gatacgaaga acacgctgat cagcgtcgcc gacacgccgc ccacggcgga tctgtccacc
      121 agcgccaacg cgagcaacga aggcgcggtt tacacgctgc acgtcggcgc gaccaccgac
      181 ccgggcccga accacatcac tggttacgcg atcaactggg gcgacggcaa cgtcgatacc
      241 ttcgccagcg ggggcgatcc ggcgaacgcc aacttcaccc acacgtatac cgatgggccg
      301 gcggcgcggc acatctcgat gacactcatc gatgaagacg gcccgcatgc caactccggc
      361 acgcacgatg tgacggtcaa caatgtcaac ccgaccgcca cgctgcagaa tttgttccca
      421 agcatcagcg aaggcgacgc cgggtcgctg aacttcacgg cccagcacga cgactcatcg
      481 gtcgataccg cctccgggtt ccactacgcc tacgacttca acaacgacgg catcttcgat
      541 tccggcgatg gttcgtacgg cggcagcggc accagctcat cgatcaacgt gccggccagt
      601 tacctgatca tccccggcca gcactcggtc aaggctcgca tcatcgacaa ggatggcgga
      661 ttcaccgatt acacgacgac gatcacgtcg gcgaacgtct cgccgaccgt caacctgccg
      721 gccaacctga cggcgggcgc gggcttgccg ttcacgttca caggcagctt ctctgatccc
      781 ggcacgtcgg atggaccatg gaccggcgtc gtcgattacg gcgacggcag cggctttgtg
      841 gcgctgagcg tcaattccgc caaccacacg ttccagctca gccacacctg ggcgaccagc
      901 gggccgtaca cgctggtggt gcacatcagc gatcacccgg tttcgccgga tggcattcaa
      961 ccgccggcgg cttcgctgac gggtgctgca tcgtcggcgg tcaacgtgac ggcgacgacc
     1021 ttccaggtca tcaacttcac gcccaccgcc agcgggttcg acgtgcagtt caaccgcggg
     1081 gcggacctgt cgaagctgaa cctgtacggg agctcgttcg gcggcttcgg cgcgtccgac
     1141 gtcagcttcg tcggcaacaa cacgggcgtt gtcgccggtt cgctcatctg ggatccatcc
     1201 accgacaccg cccatttcgt tgccactggt ggccgattgg cggcggacac ctacaacgtc
     1261 acgctcttca gccgcgctga tggctggacc gataccagca acaacgcgct ggacggtgac
     1321 ggcaacaaca ccaacggcgg cgatttcgcc cggcagttcg tgctctccta cccgctgccg
     1381 ccgcgcacgg tcagcatgcc ggacttcgcc cgcgggccgg gacagacggt cgatctgccc
     1441 aacaccagca gcggcgccgg catcccgatc acgatcagcg atgccgccaa cgtgttgtcg
     1501 gtcgatttcg acctggtcta caacaccagc ctgctgaaca tcaccggcgc cacgctggcg
     1561 tcgggcctgc cggcggattg gggtctgacg atcaacttca tctcaccggg ccggatcaag
     1621 ttcacggctt cgggcaccac gccgttgtcg gcgggggcga agcagatcat caagctgacc
     1681 gcgacggtgc cgcagaccgc cgcttacgcc ggcgccgccg acctgcagat ccagaacctg
     1741 agcgtcaacg aaggcaacat tgccagcgtc ggcgactcgg cggtggagaa ggtcgcgtac
     1801 tttggcgatg ccagcggcaa cgctgtctac accggcctgg acgctggcct gatctcgcgc
     1861 gtcgtcgtgc acctcgacag tgggttcgat gcgtatccgc tggtcgatcc ggtcatcgtt
     1921 ggcgatgtca ccggcgacgg ttcgctgtcc ggtctggatg catcgttcgt tgcccagaag
     1981 gcatcgaacc tgccgcgtcc ggaaatcccg aacatcccga ctggcttctc gatcgtcggc
     2041 ggtgggatcg atccgacgtt caccggcagc tcgcacacgc tgggccttcg tggtggtcag
     2101 gcggacttca cggtcctgct cgatcagatc accggcatca ccgacatcgg gttcacgatc
     2161 accgccagct acaccgcggg ggtcctgcac ctggcgaacg gtgacatctc gctgggcgag
     2221 gcgctgccgg ccggttggtc ggtggttgcc aataccgatg acatcaacgg caacacggtg
     2281 ctgacgttct tcaacggcgg cacgaacatg acctcggatg cgaccaagcc gatcgtgcac
     2341 cttggcttcc acgtgccggg caacgacaac ccgggcgtga caccggtgca gctgagcggt
     2401 ccatccgtcg atggcacgcc ggggcatcag gtcagttaca gctacgtcga tggttcgatc
     2461 gacatcgcgc cgttcttcac cggcaccgcc ggggacgatc agtacaccgt ccgcctgaac
     2521 ggcactggcg ataccgtgca ggtctgggag aaccagccga ccagcggcgc gccgacctat
     2581 tccttcgccg cgtcgctgtt gtcgctggcg aacttcaacc tcttcaagtt caccacgctc
     2641 ggtggcaatg acacgctgac gatcgactac tccaacggca atccgcttcc ggcgggtggt
     2701 gtgatcttcg acggtggcac gaataccgcc ggcggtgaca cgctggtgct gaagctgggt
     2761 gacggcggaa acgccgtcac cgtggacggc agcggtgtga aggtcggttc gacgccgttg
     2821 aacctgacga gcgacgagca cgtcgtcatc aacggcggca gcggggacga cagcgtcacg
     2881 cagaccgccg tgccggccgc gccgttcgcc ttcaacggtg gcgcgggcaa cgacagcctg
     2941 agcatcatgg ccggttctta caccttcacc accgatgcca gcgcgaccac ggcgagcctg
     3001 acggtgaacg tcgatggcgc cggatcgagc gtgacgttca gctcgaccga gcacctcgcg
     3061 gggctgcacc tgactggtag cgggacggcg aagatcacct cgacggctac gccgttcacg
     3121 ccgtcggtgc tgaacgttca ctcgctcagc atcgcgagcg gttcgacgtt ggatatcacc
     3181 aacaacgagg cgatcatcga cagctcgctg gcgagcattc agcccgacct gcaggccggt
     3241 cgcatcttct cgtccacacc ggttgccggt gaagggctcg gttcgcacgc cctgacaccg
     3301 acgcagtcgg tggtccggct gtcgctgctg ggtgacacca acctggacgg ctccgtcgac
     3361 gtgggtgacc tcggccagct cgcgtcgaat tacggccaga ccagcggcgc gacgtgggaa
     3421 gatggtgaca ccaacaacga cggcgcggtg gacgtcgccg acctgggcat gctggcgacc
     3481 aattatggtg ttagcctgtc gaacggcgcc gcggccccga gcagccaggc cgccgtcttc
     3541 gcgtcggcca acgttgttac cgcaccggtg gccaccccgg cggccgctgt ggcacaaccg
     3601 gcggcattgc tcagcaccac gagcacgacg atcagcacgg ccaagtcaga cgtgccggcc
     3661 gtcaccgtga cgaccaagtc cacggccaag aaggcggacc attcgcaggt ctcaggcaat
     3721 cacacccggg tggaaccctc ctcgacgctg cccctgaagc acaagggcca gaagaagggc
     3781 gacatccttg gcgatgacct gaagagcctg tgagcaacga cagggtaggt tgtgcaggaa
     3841 accgcggggt cggcgatgaa ctccaaccgg agttcacccg gccccgcggt tttttcgttg
     3901 caaaaagtgc tatcacagac gcgaacatca gtcatcccgc tactgccgct tgcggcttag
     3961 cgtctgttct tgccaggcga gagcaacaga cgctaagccg caagcggcag cattgctgaa
     4021 gcaatccccc atcccacgtt gtgtctggta ctggtataca atgtgacgtc cgcatgttgc
     4081 ggtatggata ctgccgcggg cgggatgtgc atcttcaaca ggtccggccc ccttatccca
     4141 ggaaggagcc gctgccatgc tcgtgttggc ccgcatgcgg gacgaatcga tcattattgg
     4201 tgacgagatc acggtgaccg tcgccgacat tcgcggggac aaggtccgcc tcggcatcgc
     4261 cgccccgcgg cacgtgtcgg tgcatcgcaa ggaagtttac gaccagatca ggcgcgagaa
     4321 tcaggcggcc gcggggctgc agcccggcga tgtcgccggg gcggtcaagt cgccgtcgat
     4381 gcgcgcaggc aatggcccgg caccgacggg agatacgccc gaggctcgcc tgctcctgcg
     4441 cgccgcgttg gaccaagcgc gccacagcgc tgcgggcggc gggttgccga tcggcgccgt
     4501 gttggtgcgc aacgaccaga tcatcggtag aggatgcgat cgaagcatcc agagcggcga
     4561 tccgacggcg accgcggtca tcgactgcct gcgcaacgcg gggcgacagc tggcgtacgg
     4621 ggacacggtg ctatacacga cgctgctgcc cgatcacgtt gccgccggcg ccgtggtgca
     4681 actggggatc gggcgcgtgg tgatcgggga agcggtgcac gcgcgcgatg gcgcgccgcc
     4741 caaaccgccc gtggcgaacc tgctgcgcgc ggcgcatgtc gaggtactgg atctgcacga
     4801 ttcggcgtgt gtcgagatgg tcgacacatt catgcgcgag cacccggagc ggtggcgcag
     4861 ttcctattga tcgccgtaac ccccctaaag cccgcggctc tgccgtggga gggattgctc
     4921 aagcccggtc ccacggcaga gccgtgggtt tttttgcttc tcaacgttgc gccgcgcggg
     4981 ttacattgct cgcacaggac acctctgcca tggcgggcaa cgcgccgaac atttccgaag
     5041 tccgcgccgc gatcgatcta tacctgggcc acgcattcgc cggcgacgcc gacctgccgc
     5101 acgcggtcgc ggcgatcgtt cagcagatcc gggatgctga cgacgcggag gtgttgtcgc
     5161 tgccgatgtg ggaacgcgac ggcgcccagc ccgcgtgccg ctactgcctg cggctcggca
     5221 accgctatta tccgcacatg aaactggtgc tggagcggcg gccggatggg ctgggctacg
     5281 tcctgcgcgt cgatggccac gaccagcatg tcttagcgac ccccgtcggc gcagcgtgcg
     5341 aagcgttcta cgaagtggtc cgccacgacg cggagatcgc ccgcgacatc gaagccgcgt
     5401 gggagcagca cggcctgaac acgctgcggg ggttcatcgc ctcgcacgcg cagacgacac
     5461 agcgtttttc gccagcggac gtcgtcaagg ccacgtgatc cgggcgagca cgttctattg
     5521 gtttggatca atcggcgaaa tcacgaagta ccaatgctgc cgcgtgcgcg gtttgaaccc
     5581 gcgggcgtgg taaatgcgcg ccgccgcgtc gtgaccggct tcgacttcca ggtccagcgc
     5641 gcgccacccg cggcgggtgg cctcggcgat cacctcgttc aacaattggg tgccaacgcc
     5701 ttggcgccgg gcctgtggca ggacgtacag ttcttccaac caaccgctgg cgccgccgtg
     5761 ctcgatggaa atgatcgccg atgcgtaagc gacgccgacc ggcgttgcgc cgtgccacgc
     5821 aatgaggatg aaaccgacgc gcggttcgtc ggtgatcatc cgcaccaccc gccggatcgt
     5881 cgccgggtct gtaacgattt ggtgatcgac catctgcgcg acgaacagcg cgacgacggc
     5941 atcgccatcg gcaacggatc gcaggggcga tatgaccagt tcgctgtcga tcacaatggc
     6001 tctgtagccg cgacgcctgc gtcgccttct cgcgcaaagg cgacgcaggc gtcgcagcta
     6061 caactacgat agcgccgctc agcccggaat ctccggctcg accagttgcg ccagcagtgt
     6121 gagcgattcc tgccagccga gatagcacgc ctcggcgggg atgaccgcgg ggatgccttc
     6181 ctgcacggcg ttcagttccg tgccgcagaa caccttcttc agcgtgatcg tcgtctgcat
     6241 ctcgccggcc aggctggcat cgtcgaagcg gctggtgtag cgaatgcgct cgtgggggat
     6301 cagctccagg tattcaccgc cgaacgaatg gccgccgccg ctgctgaagt tcgtgaagga
     6361 cattctccac ttgccgccga ccttggcgtc catctgatga accgtgccgg tgaatccgtg
     6421 aggcggcagc cacttggcca tcgcggcggg gtccaggaac gcgcggtaaa cccgctccgg
     6481 tgtagcccgc agcacgcgat gaaggcggat ggtattgctt ggcatgcttt gctccgttgg
     6541 tgaattcggg ttggtccaaa gtcagaaaaa aactacagcg ctcacatgtc atgagaacgg
     6601 atctgagaag cgtgtgcgac cgataatagc ccaggcccga aggccgggga tcgaattgga
     6661 aatggcgcgc ttagcagttc gatccgcggc ctttgggcct gggctttcaa cttcaccggc
     6721 tgaagaactt cttgatcgtg tagaccgtcg cttggcgttg gatgccggca atgctttcca
     6781 gcagggggat ttccttcgga cagacctcga cgcagttgcc ggctttgccg cagtcggcga
     6841 cgccgccttc ttccatcagc gtctccagcc gttcttcctt cagggccgcg cccgtcgggt
     6901 gatcgttgaa caacttcgcc tggctgatga cggcggcgcc gacgaaatgg ttgctgggcg
     6961 tgtattgcgg gcaggcttcc aggcagcagc cgcagctgat gcagcggctg agcggatagc
     7021 gctcttcctg ccgcgcttgc gacacggcgg gaccgggacc aaggtcgtag gtgccgtcga
     7081 tcggcaccca tgccttcacg cgcttcaggt cattgaacag ccgctggcga tcgacgaaca
     7141 ggtcgcgcac gagcgggaac ttgctcatcg gctccagcgt gatcggttga tcgagcttgt
     7201 cgattagcgc ggagcacgat tggcgggtct tgccgttgat cagcatcgtg caagcgccgc
     7261 agacttcttc gagacagccg ctgtcccaga ccggtggcgt cgtctgaaca ttgtcggtgg
     7321 tgaccgggtg cgcggcgatc cactgcaggc acgcgatgat gttcaggttc gggcggtaag
     7381 ggacggaaaa ctcttcccag cggtttggct tgccggggcc gtcctggcga cggatgcgga
     7441 tgatcacagc tttgcccccg gcggcatcgg cagcgcgcgg cggggtggcg tcggtgacgg
     7501 tcgggttcat gatgcccgaa tggtacgaag cgacaccgcg ggggacaagc gcggttactc
     7561 gtcgccgatc gctaacgcgc gtggttgctg aggtgtctgg tcccctctcc caggtacgcg
     7621 tgggagaggg ttagggtgag ggtgatttcg agcaccaatc gccatcgaaa tctaaatcac
     7681 cctcaaccag gcccgcgagc ctgtgcctcc tctccacggg gacgtgggag agtggcgaca
     7741 tgcaaggcgc gcgcgccgcg ccttatctcg ctagtggtgg taaacatgtt accaaggcgc
     7801 tgtcctgaca ggtctggcgg tttacggtag aacctgagcc acccttaacc caaaaggaac
     7861 cgttatgcac gattgctcgg tcagtcgttc gtcccgattc tttgcttgtg tcgctctgtt
     7921 cgcgctcgcg ctggttggcc gcgccttggc ggacactgtc ggcctggctc agctggacct
     7981 ggcgagcgtg catcaggatt tcggcaagcc cggcgtcaat aaatcgatcg acggacatcc
     8041 gatcaccatc ggcgggaaaa gcttcgaaca cggcctgggc actcacgcgc agtcggtttg
     8101 gtggatcaac gtcaatggcc gcggcgagcg gttcaccgcg tcggttggtt tggacgacga
     8161 ggtgaagtcg gacccgaagt catcgcgtgt cggcgtcgag ttcaaggtga tcggcgatcg
     8221 caagacgctg ttccgctccg gccgcatgaa ggtgggcgaa gcgccgaagc cggtcgatgt
     8281 cgatctggac ggtgtgaaga tgctgatcct ggtcgtctca ccgatcaacg gcaacatcga
     8341 ctacgcccac tgcgactggg ccgacggcac gattacatat agcggcgaac ggccggtcgc
     8401 gctcgagccg ccgccggata tgccggtggt gttgacgccg cccgcgccgg cgacgccgcg
     8461 gatcaacggg gcgaaagtct ttggcgttcg accggcgcat ccggtgctgt acatgatcgc
     8521 cgccaccggc gatcgaccga tgaccttcaa cgcggagaat ctgccacccg ggttgtcgct
     8581 ggatcagtcc agcggattga tcactggatc gaccgaaaag gccggcgaca acgccgtcac
     8641 gatcaaggcg accaacgcca agggcagcgc tgacgcccag ctgcacatca tggttggcga
     8701 tcgcatcgcg ctcacgccgc cgatggggtg gaacagttgg aattgtttcg cgacggcggt
     8761 gagcgatgag aaggtgcgcg ccgccgccga cgcgatggtg aacgcggggc tggtgaatca
     8821 cgggtggacg tacatcaaca tcgacgactg ctgggaagtg aaggccagcg aaccacccga
     8881 gaatcgccgc gacgccagcg ggcggatcaa gaccaacaac aagttccccg acatgaaagc
     8941 gctggcggat tacatccatt ccaggggact tcgcgccggt ctgtattcat cgccgggtcc
     9001 gtcaacgtgc ggcggataca ccgccagcta tcgctatgaa gagcaagacg cccgggaata
     9061 tgcggactgg ggtttcgact atttgaagta cgactggtgc tcgtacggca acatcgcgga
     9121 tcagatcaag aaggccccga acccgccggc gcagctggag atcttccagc atccgtacaa
     9181 cgtgatgcac gaggcgctgg cgaagcagaa ccgcgacatc ctcttcagcc tgtgtcagta
     9241 cggcatgggg gacgtgtggg agtggggcga tcaagtcggc gggaactgct ggcgaacgac
     9301 cggcgacatc cgcgacacct ggggcagcat ggccggaatc gggttcaaac aagccggtca
     9361 cgaaaagttc gccgggccgg ggcactggaa tgatccggat atgctggtgg tgggcaaggt
     9421 cgggtggggg ccggcgctgc
//
LOCUS       DATIMH010000420         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_387065, whole genome shotgun sequence.
ACCESSION   DATIMH010000420 DATIMH010000000
VERSION     DATIMH010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387065"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1539)
                     /locus_tag="VLI90_07215"
     CDS             complement(142..1539)
                     /locus_tag="VLI90_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HSV14032.1"
                     /translation="MVAAPSVATSSAYAPAREGSFEQTLKSARKQNPAEKDDDGAVRA
                     EKPAPKKPAKSTKGKRSVGATAGKRRTDESTDDVAEDQAASDSQTEIDKDGDVEDAAS
                     PTTDAPEKHDEVPKAAVQAQPALAVAQAPVTEALPGPAADGKTTGATGTEATTAAAQQ
                     KIKAVAAKASADAKVEDADSDAENAEPDATDAVSATDGAKIAKSANAPTSPAGIELGT
                     SKVKSAGLVTHESANPDAANAAASSAAAAAAAPAVSSDDLAATISSALDSVTSSIDGG
                     DSSASSRHASTNVDGGANVGPFEQVMQREISTMTAAKAQAPDAMPPEARFVDVNHPAI
                     LSGIHSQLLPNGGTMQIRLDPPELGALQVTVHMRDGIMTAAFETSNDDATRMLSHSLN
                     ELKSMLEAGGVNVERMHVQQAPRPESHGSGSDQQQQRGGEEQSGAKQEQQRRALLQRM
                     WRKLTNGSDPLDLVA"
BASE COUNT          210 a          563 c          523 g          380 t
ORIGIN      
        1 gcgtgccgct cgaaccactg gtgacggagg aggtggtcat gtggttgatt tcgtccgcga
       61 attgagcggt agttcaaaaa ttggatgcgg taatctggcg cgcgccgatt gttgcgcggc
      121 cggagcgcag ctcctgccga atcaggcgac cagatcgagc ggatcgctgc cgttggtgag
      181 cttgcgccac atgcgctgca gcagagcccg gcgttgctgt tcctgcttcg cgccgctctg
      241 ctcttcccca ccgcgttgtt gctgctgatc gctgccgctg ccgtgcgatt ccggccgcgg
      301 ggcctgctgc acgtgcatgc gctcgacgtt cacgcccccc gcttccaaca tgctttttag
      361 ttcgttcaag ctgtggctca gcatccgcgt ggcgtcgtcg ttcgatgttt cgaacgcggc
      421 cgtcatgatg ccatcgcgca tgtgcaccgt gacctgcaac gccccaagtt caggcgggtc
      481 caggcgaatc tgcatcgtcc cgccattggg caacagctgc gagtgaatgc cgctcaggat
      541 cgccgggtga ttgacgtcga cgaagcgcgc ttccggcggc atcgcgtccg gcgcctgcgc
      601 cttcgccgca gtcatcgtgg aaatctcgcg ctgcatgact tgctcgaatg gcccgacgtt
      661 cgccccgcca tcgacatttg tgctggcgtg ccgtgacgag gcgctgctat cgccgccgtc
      721 gatgctgctg gtgacggaat cgagcgcgct ggagatcgtc gccgccaggt catcgcttga
      781 gacggccggc gccgccgcgg ctgccgccgc tgaagatgcc gccgcgtttg cggcgtcggg
      841 gttcgcactt tcgtgggtca ccaagccggc ggatttcacc tttgacgtcc ccagctcaat
      901 gcccgcgggg gatgtcggcg cgtttgctga ctttgcgatc tttgcgccgt ctgttgcgct
      961 gactgcatct gtcgcatccg gctcggcgtt ctctgcatca ctatccgcat cttcgacctt
     1021 cgcgtcagcc gatgccttcg cggcgacagc cttgatcttc tgctgtgcgg cggcagtggt
     1081 cgcttcggtg ccggtcgcgc cggtcgtctt accgtcggca gcaggcccgg gaagtgcctc
     1141 cgtcactggc gcctgtgcca ccgcgagcgc aggttgggcc tgtaccgccg ccttcggcac
     1201 ctcgtcatgc ttctccggcg catccgtggt tggactagcg gcatcctcga catcgccatc
     1261 cttatcgatc tcagtttgag aatcgctcgc cgcctgatct tccgccacgt cgtcggtgga
     1321 ttcgtcggtc cgcctcttgc cagcagtcgc ccccaccgac cgcttgccct ttgtggattt
     1381 cgccggcttt tttggcgcgg gcttttccgc gcgcaccgcc ccatcatcat ccttctccgc
     1441 cgggttctgc tttcgcgcgc tcttgagcgt ctgttcgaat gaaccttccc tggcgggcgc
     1501 atacgcgctg ctcgtcgcca cgctgggggc ggccaccatg gtggtcggcg acgcgaataa
     1561 tccgatcgaa ttgctcttag ccatcacgct ttattccttt gccgacgcct gcggcgatgc
     1621 aggcggcgtt gtgttcgacg ttgccggcga cgcgggcgaa gcaccgcccg gcgatg
//
LOCUS       DATIMH010000421         4693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1255506, whole genome shotgun sequence.
ACCESSION   DATIMH010000421 DATIMH010000000
VERSION     DATIMH010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255506"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1614)
                     /locus_tag="VLI90_07220"
     CDS             complement(<1..1614)
                     /locus_tag="VLI90_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HSV14033.1"
                     /translation="MTKSPHGKLQTLDLQDVSWTTGFWADRARVLRENLIPTMARLMS
                     ESDRARFVGNFEVAAGLVEGRHRGPKWNDGDFYKWVEAGCIVLATHPDAALSAQLDEL
                     IALIAKAQQADGYIHTDITIRQKAGEDVKRFGNPMDFEMYNMGHLMLAGIAHHRATGR
                     TSLLDVAIKSADFLCRQFESPTAEQARHGICPVHLTGLIELFRYTGEARYRDLAVTLL
                     NMRDLVTRGDDDNQDRVPFRQQTEAVGHAVRATYLYTGAGDIYLETGDPSLLPPLKTI
                     WNDLVTRKLYITGGCGALFDGASPDGVEQQATITRVHQAFGRAFQLPNSTAHNETCAA
                     IGSVLWNWRMLLITGESRFADLLEHTLLNSVLAGVSLDGTAFFYTNSLRQLHEMPAPL
                     RWNRHRKHHINCFCCPPNVARTLAQANQYAYAKDDVNIYAIVYGSNTLDTSIAGGRVR
                     LRQETDYPWNGSIRITIDHSPEHPFSLHLRIPAWAGAATVRVNGAEERSQRPRPGSFY
                     RITRQLKTDDVIELDLPMPARLMEANPLVE"
     gene            complement(1740..>4693)
                     /locus_tag="VLI90_07225"
     CDS             complement(1740..>4693)
                     /locus_tag="VLI90_07225"
                     /inference="COORDINATES: protein motif:HMM:NF040048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HSV14034.1"
                     /translation="DAPVKVTLAPLPGDNTARVQAAIDYVALQPPDARGIRGAVLLLK
                     GPFDIGGALTINTSGVVLRGQGTGANGSVLIASGLDRRTLVTIAGRGDREMTGKSIAI
                     AAHYVPVNAMQLPLDSAASLKVGDTVIIRRPCTREWIDALGMRDLGGGMGNGWRPGSR
                     ELAWDRTITAINGNTITIDAPITCALDPNFGGGTVTAYKWPGRISQIGIEGVRCESTF
                     DPKNAKDEAHSWIAIAMDNVRDAWVRQVTFSGFAGSAVTLLDSTSRITVQDCKSLSPV
                     SEIGGYRRHTFYTAGQQTLFERCYSERGWHDFAAGFCAPGPNAFVQCEATDALNDSGP
                     IDSWACGTLYDNVRVDGAALSLGDRRYNNFFAGWAAANSVIWQCNASVINCFDPPGAH
                     NWCFASWGVFNGDAGFWASDNTVAPASLYYGQLVDRRGPSAERTAFIMPRNSQSSRNP
                     TLDNAAKLTAESVNPAPSFPAWIDAATQRTPLDFDAHGAKSLDEIAPPSRPVVEKPRL
                     DHHVTVTNGWLTDNRALVIGARQGIKWWRGGIRPDEVAEAEKDGPNPTRFVPGRIGPG
                     LTDDLKTIADQMIAGGRVALDYHYGLWYDIRSIDHERVRRINGDVWPPFYEMPFARTG
                     GNELDWNGLSKFDLTQYNAWYWSRLKQLADIFDQRGLVLFNENFFQHNLLEAGAHWAS
                     CPWRSANNINNMGFPEPPPYAGDKLIFMADQFYDETNPNRRPILRAYIRKCLDNFADN
                     SNVIQFTSAEYTGPAHFMRFWLDTIGEWEKERGRHPMIGLAAPKDVQDEVLAEPARAA
                     VVDVIDIRYWWYQPNGELYAPPGGKNLSPRQWSRGPKFPNFESAYRAVREVRSRFPDK
                     AVLIGPEAGSANFPWAVLMGGGSMPPFKHLDAKLATLVATMRPIDSAPGDAKQWMLGD
                     GGKSFLIYCQSNEPAKLDLPRGGAMYSVSRVNLEDGSLTAMGDAPRGEGIVEIKNSGA
                     NGPYALLVQVK"
BASE COUNT          825 a         1519 c         1463 g          886 t
ORIGIN      
        1 ttcgacgagc ggattcgcct ccatcaatcg cgccggcatc ggtagatcca gctcgatcac
       61 gtcgtccgtc ttcaattgtc gagtgatgcg atagaacgac cccggccgcg ggcgttgcga
      121 tcgctcttca gcgccgttca cgcgaacggt ggccgccccg gcccacgcgg gaatgcgaag
      181 gtgcagcgaa aacgggtgct cgggcgaatg gtcgatcgtg atgcggatcg atccattcca
      241 cggataatcc gtctcctgcc gcagccgcac gcggccgcct gctatcgacg tgtccagcgt
      301 gtttgagccg tagacgatcg cgtagatgtt cacatcgtct ttcgcatagg catattgatt
      361 cgcctgcgcc agcgtccgcg ccacgttcgg cgggcagcag aagcagttga tgtgatgctt
      421 gcgatggcgg ttccagcgca gcggcgccgg catctcgtgc agttggcgca gcgagttggt
      481 gtagaagaac gcggtgccgt cgaggctgac gccggccagc acgctattga gcagcgtgtg
      541 ttccagcaga tcggcgaagc gcgattcgcc ggtgatcagc agcatgcgcc agttccacag
      601 cacgctgccg atggctgcac aagtttcgtt atgcgccgtg ctgttgggca gctgaaacgc
      661 ccggccgaac gcctggtgaa cgcgcgtgat cgttgcctgc tgctcgaccc catccggcga
      721 tgcgccgtcg aacagcgcgc cgcacccgcc ggtgatgtag agcttgcgcg tgaccagatc
      781 gttccagatc gtctttagcg gcggcagcag cgaagggtcg cccgtctcca gataaatgtc
      841 gcccgcgccg gtgtacaggt acgtcgcgcg aacggcatga ccgactgcct cggtctgctg
      901 gcggaacggc acgcggtcct gattgtcgtc atcgccccgc gtcaccaggt cgcgcatgtt
      961 cagcaacgtg acggcgagat cgcgatagcg cgcttcgccg gtgtagcgga acaactcgat
     1021 caggccagtg agatgcaccg ggcaaatgcc gtggcgcgcc tgctcggcag taggggactc
     1081 gaattgtcga cagagaaaat ccgctgactt gatcgcgaca tcgagcagcg acgtgcggcc
     1141 ggtcgcgcgg tggtgggcga tgccggcgag catcaagtgc cccatgttgt acatctcgaa
     1201 atccatggga ttgccgaagc gcttcacatc ctcgccggcc ttctggcgga tcgtgatgtc
     1261 ggtgtggatg tatccatccg cttgctgcgc cttggcgatc agcgcgatca gctcgtcgag
     1321 ctgcgcgctc aatgcagcat ccggatgcgt cgccagcacg atgcacccgg cttcgaccca
     1381 cttgtagaag tcgccgtcat tccacttcgg cccacggtgc cggccttcga ccagcccggc
     1441 ggcgacttcg aagttgccga cgaagcgggc gcgatcggat tcgctcatca atcgggccat
     1501 cgtggggatc agattttcgc gcagcacgcg cgcacgatca gcccagaagc cggtggtcca
     1561 ggacacgtcc tgcagatcca gggtctgaag tttgccgtgg ggacttttgg tcatgctacg
     1621 cggtgctcgc agatgcgctt caacttgata gccgcaggcg atagccaacg ggggcgcggg
     1681 aagcgaaaac ccgctggctt tcgcctgcgg ctatcaaaga ggctcatgat cgaatgattt
     1741 catttcactt gcaccaataa agcgtagggg ccgttcgccc ctgaattctt gatctcgacg
     1801 attccttcac cgcggggcgc atcgcccatc gccgtgagtg atccgtcttc cagattcacg
     1861 cgggaaacgc tatacatcgc cccgcccctg ggcaggtcga gcttcgcggg ttcattcgac
     1921 tgacagtaga tcaaaaaact ctttccgccg tcgccaagca tccactgctt tgcgtcgccc
     1981 ggggccgaat cgatcggccg catcgtcgcg accagcgtcg ccagcttcgc atcgagatgc
     2041 ttgaacggcg gcatcgaccc accgcccatc aggacggccc acgggaaatt cgccgagccg
     2101 gcctcgggtc cgatcaatac cgccttgtct gggaacctgc ttcgaacttc gcgaaccgcg
     2161 cgatacgccg attcgaaatt cggaaacttc ggtccccggc tccattgccg cggcgataaa
     2221 ttcttcccgc ccggcggggc gtacagttcg ccattgggtt gataccacca gtaacgaatg
     2281 tcgatcacgt caacgaccgc cgcccgcgcg ggctcggcca gcacctcatc ctgcacgtcc
     2341 ttgggcgcgg ccagcccaat catcggatgc cggcccctct ccttctccca ctcgccaatc
     2401 gtgtccagcc aaaaccgcat gaagtgcgcg gggccggtgt attcggcgct ggtgaactgg
     2461 atgacgtttg agttgtccgc aaagttatcc agacatttgc gaatgtacgc gcgaaggatc
     2521 ggccggcggt tcggattcgt ctcgtcgtaa aactgatcgg ccatgaaaat gagcttgtcc
     2581 cccgcgtacg gtggcggctc gggaaagccc atgttgttga tgttgttcgc cgaccgccac
     2641 gggcaactgg cccagtgcgc gccggcctcc agcaggttgt gctggaaaaa gttttcgttg
     2701 aagagcacca gtccgcgctg atcgaaaatg tccgccagct gcttcagccg cgaccaatac
     2761 cacgcgttgt actgcgtcag gtcgaacttg ctcaggccgt tccagtccag ttcattgccg
     2821 cccgtgcgcg caaacggcat ctcgtaaaac ggcggccaga catcgccgtt gatccggcgg
     2881 acgcgctcat gatcgatgct gcggatgtcg taccaaaggc cgtaatggta atcgagcgcg
     2941 acgcgcccgc cggcgatcat ctgatcggcg atcgtcttca gatcatccgt caatcccggt
     3001 ccgatccgcc ccggcacgaa gcgggtgggg ttcggcccgt ccttctccgc ttcggccact
     3061 tcgtcgggac gaatcccgcc gcgccaccac ttgatgcctt gccgcgcgcc gatgacgagc
     3121 gcgcgattgt ccgtcaacca gccgttggtg acggtgacgt gatgatcgag ccgcggcttc
     3181 tccaccaccg gtcgtgaggg cggtgcgatt tcatcgagcg atttggcgcc atgcgcatcg
     3241 aagtcgagcg gcgttcgctg cgtcgcggcg tcgatccacg cggggaacga cggcgcggga
     3301 ttgaccgact ccgccgtcaa cttcgccgcg ttgtccagcg ttggattgcg cgacgactgg
     3361 ctgttccgcg gcatgatgaa cgccgtgcgc tccgcgcttg gcccgcggcg atcgaccagc
     3421 tggccgtaat acaagctcgc gggcgcgacg gtgttgtcgg acgcccagaa accggcgtcg
     3481 ccattgaaga cgccccagga tgcgaagcac cagttgtgcg cgcccggcgg atcgaagcag
     3541 ttgatcaccg aggcgttgca ctgccagatg accgagttgg cggctgccca gccggcgaaa
     3601 aaattgttgt atcggcggtc gcccaggctc agtgccgcgc cgtccacgcg aacgttgtcg
     3661 tacagcgtgc cgcacgccca gctgtcgatc gggccgctgt cattcagcgc atcggtcgct
     3721 tcacattgca cgaacgcgtt tggacccggt gcgcagaaac ccgcagcgaa atcgtgccag
     3781 ccacgctcgc tgtagcaacg ctcgaagagc gtctgctgcc ccgcggtgta aaacgtgtgc
     3841 cggcgatacc cgccgatctc cgagaccggc gacagcgact tgcaatcctg caccgtgatc
     3901 cggctggtcg aatcgagcag cgtcaccgcc gatccggcga aaccgctgaa cgtgacctgc
     3961 cgcacccacg catcgcgcac gttgtccatc gcgatcgcga tccaagagtg cgcttcgtcc
     4021 ttggcgttct tcggatcgaa cgtggactcg cagcgaacac cttcgatgcc gatctggctg
     4081 atccgacccg gccacttgta cgcggtgacg gtgccgccgc cgaagttcgg gtcgagcgcg
     4141 caggtgatcg gcgcgtcgat cgtgatcgtg ttgccgttga tggcggtgat cgtgcggtcc
     4201 cacgccagct cgcgcgaccc cggccgccaa ccgttgccca tcccgccgcc gaggtcgcgc
     4261 atgccgaggg cgtcgatcca ctctcgcgtg catggccggc ggatgatcac cgtgtcgcca
     4321 actttcaacg aagccgcgct atccagtggc agctgcatcg cgttcacagg cacataatgc
     4381 gcggcgatgg cgatcgactt gccggtcatc tcgcgatcgc ctctgcccgc gatggtgacc
     4441 aacgtgcggc gatcgagccc gctggcgatc agaacggatc cattggctcc tgtgccttgt
     4501 ccccggagga cgacgccgga ggtgttaatc gtcagtgcgc cgccaatgtc gaatggaccc
     4561 ttgagcagca gcaccgcacc gcgaatgccg cgcgcgtcgg gcggctgcaa ggcgacgtag
     4621 tcgatggcag cttgaacgcg cgcagtgtta tcgcccggca gcggcgcgag ggtgactttc
     4681 accggcgcat cgg
//
LOCUS       DATIMH010000422         4382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2695984, whole genome shotgun sequence.
ACCESSION   DATIMH010000422 DATIMH010000000
VERSION     DATIMH010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2695984"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VLI90_07230"
     CDS             complement(<1..827)
                     /locus_tag="VLI90_07230"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV14035.1"
                     /translation="MKRLHRHKGFTLVELLVVIGIIALLISILLPSLNRARETANRVK
                     CASNLRQIGQAILLYANENKQQYPRTVLGTAAAASAPQPTWGTGNQTATTPDPFTTTP
                     AHPESDNDVSAALFLLLRTQDITSEVFVCPSSNAEKWDFTGGANTAQNFVNWISVQKN
                     LSYSYENPYPGGTAIANGWKLNTSLPADYAVASDINPGSTIPNGTLNPTSVDTTSPAS
                     QLRLGNSANHEQDGQNVLYGDGHVEFQNNPFCGTQRDNIFTGRGSSTTMTNPFSSGGV
                     "
     gene            complement(1259..1801)
                     /gene="coaD"
                     /locus_tag="VLI90_07235"
     CDS             complement(1259..1801)
                     /gene="coaD"
                     /locus_tag="VLI90_07235"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSV14036.1"
                     /translation="MSRPRKIAVFPGQFDPITNGHIDVIRRGVALFDELVVAVGVNPD
                     KRELFTVDERVSMISHLLKDVDGHVSVEKYGGLTVDFVRQAKATAILRGIRDASDLRY
                     EFQLAMANRAVGGVETVFIMTGDQFALTSSSLIRQIVSLGGDVRALNTILPAVVVQKL
                     REKQRRGRLEVSTEPDTPSS"
     gene            1887..2672
                     /gene="pgeF"
                     /locus_tag="VLI90_07240"
     CDS             1887..2672
                     /gene="pgeF"
                     /locus_tag="VLI90_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HSV14037.1"
                     /translation="MLERRESAEGPVYYVSPLLQRAGIRHAFSTRLGGVSPAPFDSLN
                     LGNPGGCDRQDDDERIQANYRRLQAALGCVHSTRCWVHQVHGGDVADANSGFSNGIKA
                     DALLTNDPSQVLAIRIADCVPILVADEQGKTVAAIHAGWRGVIAGIIPATLARMPDMN
                     SLIAAIGPCIGFDAFEVGHEVLREFDRAFGDEAPVRRNGNGKGHVDLRAACHLQLRRA
                     GLSDEQIDTTDRCTFRDADEFFSHRRDRGVTGRLAALIAPRSR"
     gene            2669..4378
                     /locus_tag="VLI90_07245"
     CDS             2669..4378
                     /locus_tag="VLI90_07245"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV14038.1"
                     /translation="MNVLNPTSAQPPRHRIALSLLVAAITFLAFARLIACDFTTLDDP
                     DTIANNPRLRPPTLANVAHYWTATGEDAPFGLYIPVTYATWSAVAAVAGINPHAFHAA
                     NVLLHTLSALLVFHLLLRLFNCGAPAVIGALLFALHPVQVEPVAWASGTKDVLCGCLS
                     IAALLEYINFAACGLASVSASSPPDEQTLSRKRRGNIHFTIATICFILAMLAKPTAIV
                     VPLLALTIDRLLLQRDWSQILRWLWAWFTLMIPCAIWTTRAQPAPWSTSLPVWTRPAI
                     ATDAIAFYLYKLALPLHLIVDYGRRPQAVLDHGWIYWTWLVPAAIALFLIIRRPRFIT
                     AGACLFVIPLLPVLGLAPFMYQYVSTVTDHYLYLSLLGPAVLLAWLVQRSPRVMPLAL
                     ILLLLLGGRTIAQEQYWQDTPTLFEHTLAINPNSFLACDQLGFYHAELARKLRWSSPA
                     AAREQFAQAVGYYQRGLTIHPQYVPSIYNLAIVYRQIGREDDARAMLRRIVQLQPSLP
                     PGLRADPIGLADRLLIYNDPATAVDWLDQVLRNNPTNARAQQLRQIALARAATRPVTP
                     PTP"
BASE COUNT          787 a         1461 c         1277 g          857 t
ORIGIN      
        1 acaccacccg agctgaacgg gttggtcatc gtggtgctgg aaccacggcc ggtgaagatg
       61 ttgtcgcgct gagtgccgca gaacgggttg ttctggaact cgacgtgacc gtcaccgtac
      121 aggacgttct ggccgtcctg ctcgtggttg gcgctgttgc ccaggcgcag ctggctggcg
      181 gggctggtcg tgtccacgct cgtcgggttc aacgtcccgt tcgggatggt cgaaccgggg
      241 ttgatgtccg acgcgacggc atagtcagcc ggcagcgacg tgttcagctt ccagccgttg
      301 gcgatggccg tgccacccgg atacggattt tcgtaactgt agctcaggtt cttctgaacg
      361 ctgatccagt tcacgaagtt ctgggcggtg ttggcgccac cggtgaagtc ccacttttcc
      421 gcgttgctgc tcgggcagac gaacacttcg gacgtgatgt cctgggtgcg cagcaggagg
      481 aacagagcgg cgctgacgtc gttgtcgctc tcggggtggg ccggcgtcgt cgtgaagggg
      541 tcaggcgtgg tggcggtctg gttgccggtg ccccaggtgg gctgcggagc agaagcagcc
      601 gccgccgtgc ccagcaccgt gcggggatac tgctgcttgt tttcgttggc atacagcagg
      661 atcgcctgac cgatctgacg caggttgctc gcgcatttca cgcggttagc cgtctcacga
      721 gcacgattga gcgacggcag caggatcgag atcagcagcg cgatgatgcc gatcacgacc
      781 agcagttcga caagggtaaa acccttgtga cgatgaagac gcttcataaa gaagctcctt
      841 tcatgaggcg tcgcagtcgt ccgaggcaaa tcaaacgcga gcggtcgggt cgaaggatcg
      901 gataatagag atgattgcac cgttcagctt ttcgacgaaa agctggattt ttcgaccttg
      961 tgctctgctg gattcacttc agcgccacga cattgggaat gacaccggcg aacaccgccg
     1021 atgaatcatt gcaaatttag catggggaaa aacggttccg aaacctctga atactctcgc
     1081 cgagaatgag caagctcaac gcgacctgtt ttaccgccgc cgcggctggg gacaagtttt
     1141 gctccgccat acaacagttg agcggcgtgt aaagtagatc gctgggccat tgtcgtcaag
     1201 caaaatcgca ttttatgcct cgataatctt cgccatctta acggagctca tccgttgctc
     1261 atgacgacgg tgtgtccggt tcggtcgata cctcaagccg gcctcggcgc tgcttttcgc
     1321 gcagcttttg aaccacaact gccggcagaa tcgtgtttaa cgcgcgcaca tcgccaccca
     1381 gcgatacgat ctgccggatc aggctgctgc tcgttagcgc aaattggtcg ccggtcatga
     1441 tgaacaccgt ctcgacccca ccgaccgctc ggttggccat cgccagctgg aactcgtatc
     1501 gcaaatcgga agcatcccga attccacgca aaatggccgt cgccttggct tgacggacga
     1561 agtccactgt cagcccgccg tacttctcca ccgagacgtg gccgtccacg tccttcagca
     1621 agtgcgagat catcgacacg cgctcgtcta ccgtgaacaa ctcgcgcttg tccgggttta
     1681 cgcccacggc aacgacgagt tcgtcgaaca gcgcaacccc ccgccggatc acgtcgatgt
     1741 gaccattcgt gatcggatcg aactgcccgg ggaacacggc gatctttcgc ggccgactca
     1801 tcgaacctcc atctttgaag tctatcggaa aggcatgctc cggtggggga aaagtcctcg
     1861 ctgcggcgat ataaccgcat ccgcccatgc ttgaacgacg cgaatctgcc gaaggaccgg
     1921 tctattacgt ctcgccgctg ctgcaacgcg ccggcatccg ccacgcgttc agcacgcgcc
     1981 tgggcggagt cagccccgcg ccgtttgact cactcaacct cggcaacccc ggcgggtgcg
     2041 accggcagga tgacgatgag cgaattcaag cgaactatcg ccgcttgcag gcggcgctcg
     2101 gttgcgtcca ttcgacccga tgctgggtcc accaagttca cggcggcgac gtcgccgatg
     2161 caaatagcgg gttcagcaac ggcatcaaag ccgatgccct cctgactaac gacccgagcc
     2221 aggtgttggc gattcgcatc gccgactgcg tgccgatact cgttgcagac gaacagggca
     2281 agacggtcgc cgccattcat gcggggtggc gcggcgtcat cgccggcatc atcccggcga
     2341 cgctggcgcg catgcccgac atgaacagtc tcatcgctgc tatcggcccg tgcatcggat
     2401 tcgacgcatt cgaagtcggc cacgaagtgc tgcgcgaatt cgatcgagca ttcggcgacg
     2461 aggcacccgt ccgccgcaac ggcaacggca agggccacgt tgaccttcgc gctgcctgtc
     2521 atcttcaact gcgtcgtgca ggcttgagcg acgaacagat cgacacgacc gaccgctgca
     2581 cgtttcgcga cgcggacgaa ttcttctccc accgccgcga ccgcggtgtc accggacggc
     2641 tggcggcgct gatcgcgccg cggagccgat gaacgtgctc aatccaacgt cagcgcagcc
     2701 gccgcgccat cgcatcgcac tctcactgct cgttgcggcg atcaccttcc tcgccttcgc
     2761 ccgtctcatc gcctgcgact tcaccacgct ggacgacccc gacacgatcg ccaacaaccc
     2821 acggctccgc ccgccgacgc tggcgaacgt cgcccactac tggaccgcca ccggcgaaga
     2881 cgcgccgttc ggcttgtaca tcccggtgac gtacgcgaca tggagcgccg tggccgccgt
     2941 cgcggggatc aacccgcatg cgttccacgc ggcgaacgtg ctgctgcaca cgctgagcgc
     3001 tttgctggtg tttcacctgc tgctgcgctt gttcaattgc ggcgcccccg ccgtcatcgg
     3061 cgcgctgctg ttcgcgctgc atccggtgca ggtcgagccc gtcgcgtggg cctcgggaac
     3121 caaagacgtg ttgtgcggat gtctcagcat cgcagcgctg cttgagtaca taaatttcgc
     3181 cgcttgcggc ttagcgtctg tttctgcttc gtccccacca gacgaacaga cgctaagccg
     3241 caagcggcgg ggaaacattc atttcaccat cgcgacgatc tgcttcatcc tggcgatgct
     3301 cgccaaaccc accgcgatcg tcgtcccgct gctggcgctg accatcgacc gcctcctcct
     3361 gcaacgcgat tggtcgcaaa tcctccgctg gctctgggcg tggttcacgc tgatgatccc
     3421 ctgcgcgatc tggaccaccc gcgcccaacc cgccccgtgg agcacctcgc tgcccgtgtg
     3481 gacgcgcccc gcaatcgcca ccgacgccat cgcattttac ctgtacaagc tcgcgctgcc
     3541 gctgcacttg atcgttgact acggccgccg cccgcaggcg gtgctcgacc acggctggat
     3601 ctactggacc tggctcgtcc ccgcggcgat tgcgctcttc ctcatcatcc gccggccccg
     3661 attcatcacc gccggcgctt gcctgtttgt gatccccctc ctgccggtcc tgggcctcgc
     3721 gccgttcatg taccaatacg tctccacggt taccgatcat tatctctatc tctcgctgct
     3781 cggccccgcg gtgctcctcg cgtggcttgt gcagcgatca ccacgcgtga tgccgctcgc
     3841 gctgatactc ctcctactgc tcggcggccg aacgatcgcg caggagcaat attggcaaga
     3901 cacaccgacg ctgttcgagc acacgctggc gatcaatccc aacagcttcc tcgcgtgcga
     3961 ccagctcggt ttttaccacg ctgagctggc gcgcaagctg cgatggtcgt cgccagcggc
     4021 cgcgcgcgag cagttcgcgc aggccgtcgg ttactaccag cgcgggctga caattcaccc
     4081 gcagtacgtg ccgtcgatct acaacctcgc gatcgtctat cgccagatcg gccgcgaaga
     4141 tgatgcccgc gcgatgcttc gccgcatcgt acagcttcag ccgtcactgc cgccgggact
     4201 gcgcgctgat ccgatcggcc tcgcggaccg attgctcatc tacaatgacc ccgcaaccgc
     4261 cgttgattgg ctcgatcaag tgctgcgaaa caatccgacg aacgcgcggg cgcagcaact
     4321 gcgccagatc gcgctggcgc gtgccgccac acgtccggta acaccgccga cgccttaata
     4381 gc
//
LOCUS       DATIMH010000423         3805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3274269, whole genome shotgun sequence.
ACCESSION   DATIMH010000423 DATIMH010000000
VERSION     DATIMH010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3274269"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1839
                     /locus_tag="VLI90_07250"
     CDS             4..1839
                     /locus_tag="VLI90_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV14039.1"
                     /translation="MPLQIDPSKPGGRGSHFISVLALLKPGVSVAHAQEEMVRYAKQF
                     AESRGPAGHPFDPKNHPIVLAGFQDEIVKGVRSAMLVLLGAVGFVLLIACVNVANLLL
                     ARAEARRREIAVRTAIGAGMGRLLQQFIIEGVLLSLTGAIFGIGLAFAGLRLLVASNA
                     GSIPRVAEIGIDWQVLLFTLAISVATGVAFGLAPVIHMRSSKLHDTLKAAVGRATGAV
                     AANRFRAALVVSELALALVLLIGSGLMVKAFWKLQEVDAGFNPNHLLTMRLSLPAVTY
                     KDAASVNGFYSSLAERLNALPGVVSASIASGLPPERTINANDTAIEGFVPVPNGPIQN
                     IDYWNSVSGKYFDTIGAKLLEGRFLNETDGASAPLSVVVNHTMARTFWPRESALGHRV
                     RTSNNTPWRTIVGVVADVKNAALDRPAGTELYFPFAQAPRTLSWVVVRTAGEPMKLTG
                     AVRDAIRAQDRGLPISNIGSMEDVMSTARSRPRFLTLLLTLFSSLSLILAALGIYGVI
                     SYAVAQRTNEIGIRMALGARGGDVIRLIGASGVRLALAGTLLGAAGAFALTRFLSGML
                     FGVSSLDPVTFLAMAGTLILVTLLACYVPARRASKVDPLIALRYE"
     gene            1959..2366
                     /locus_tag="VLI90_07255"
     CDS             1959..2366
                     /locus_tag="VLI90_07255"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HSV14040.1"
                     /translation="MPDAVCYHCKQTLTPGEPHDCWTTTEAALTKGLSEELLDAWERL
                     RETAAEFGEQRIYASHKSIMFSRKSCYFFVRPKKKMLEICVFLDREVKAPQIRRADRV
                     SKTKVSHSIPITHRDQVESPVTDWLREAYDLSQ"
     gene            complement(2367..>3805)
                     /locus_tag="VLI90_07260"
     CDS             complement(2367..>3805)
                     /locus_tag="VLI90_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSV14041.1"
                     /translation="TPAAPANTSAGLMRATFRGGYTIAVYKGDASTGDAQEIWHNQAN
                     DPLVTNLTGARLAGEYLIFQFGAGFGGRGGGRGAAAAATPQTPETPADEWERYYSIHV
                     TDAAARPVLLTTTDGMIENQTSIALSADGKTFYYCTNAQDIERRHIWSVPVSGGKPAQ
                     ITTGEAVETSPAPLASGKYLATLSAAWNMPQSLGIWNIAAGAPATQKIIFPTTLAGFP
                     AEAHVKPEIVLTKAADGMEIHNQLFLPKDLKPGERRPAIVFVHGGPQRQMMAAYHYMQ
                     FYHWAYGINQWLANQGYVVLSVNYRSGVGYGRSFRNAPKTNAAGNSEYQDVLAGGKYL
                     QTRADVDPNRVGIWGLSYGGLLTAQALARNSDIFKAGVDLAGVHLYGSSLDPAALSYQ
                     SSAVGAIDGWKSPVLLIQGDDDRNVAFQQMTGLVQLLRQRDVYFELIVFPDDVHESLL
                     HSRWLYTLDRMEKFLHKFLWELPVTATK"
BASE COUNT          655 a         1110 c         1292 g          748 t
ORIGIN      
        1 tgggtgccgc tgcagatcga tccgtcaaaa cccggcggcc gcggcagtca cttcatcagc
       61 gtactggcgt tgttgaagcc tggcgtgtcc gtggcccacg cgcaggagga gatggtgcgc
      121 tacgccaagc agttcgccga gtcgcgcggt cccgcgggcc atccgttcga ccccaagaat
      181 cacccgatcg tactcgccgg tttccaggac gagatcgtga agggcgtgcg ctccgccatg
      241 ttggtgctgc tgggcgcggt gggcttcgtg ctgctgattg cgtgcgtgaa tgtcgccaat
      301 ctgctgttgg cgcgggcgga ggcgcggagg cgcgaaatcg cggttcgcac ggcaatcggc
      361 gcggggatgg ggcggctgct gcagcagttc atcattgaag gcgtgctgct ttcgttgacg
      421 ggcgcgatct tcgggattgg cctggcgttc gcgggactgc gcctgctggt ggcgagcaat
      481 gcaggcagca ttccgcgggt ggccgaaatc ggcatcgatt ggcaggtgct gctgtttact
      541 ctggcgatca gcgtggccac gggagtggcc ttcggactcg cgccggtgat ccacatgcgg
      601 agctcgaagt tgcacgacac gctcaaggcg gcggtgggtc gcgcgaccgg cgcggtggcc
      661 gcgaacaggt ttcgcgccgc gctcgtggtc agcgaactgg ccctggcgct ggtgctgctg
      721 atcggttcgg ggctgatggt gaaggcattc tggaagttgc aggaggtgga tgccgggttc
      781 aatccgaatc acctgctaac gatgcggctc tcgctgcctg cagtgactta taaagatgcc
      841 gcgagcgtga atggatttta cagttcgctg gcggagcggc tgaatgcgct gccgggcgtg
      901 gtgtccgcgt cgattgcgag cggcttgccg ccggagcgca cgatcaacgc gaacgacaca
      961 gccatcgaag gtttcgtgcc ggttccgaac gggcccatac agaatatcga ctactggaat
     1021 tcggtatcgg ggaagtattt cgatacgatc ggcgccaagc tgctggaagg ccgctttctg
     1081 aacgaaaccg atggcgcgag cgcgccgctc agcgtcgtag tgaaccacac gatggcgcgg
     1141 acgttttggc cgcgcgagag cgcgctcggg catcgcgtgc ggacgtccaa caataccccg
     1201 tggcgcacca tagtgggcgt ggttgccgac gtgaagaacg ccgcgctgga tcgtccggcg
     1261 ggaaccgagt tgtacttccc cttcgcgcag gcgccgcgta cgctcagctg ggtggtggtg
     1321 cgcacggcgg gcgagccgat gaagcttacc ggcgcggtgc gcgatgccat ccgcgcacag
     1381 gatcgaggcc tgccgatttc gaatataggg tcgatggaag atgtgatgtc gacggcgcgg
     1441 tcgcgtccgc gatttttgac gctgctctta acgctgttct cttcgctttc gctgatactt
     1501 gcggcgctgg gcatttacgg cgtgatttcg tatgcggtgg cgcagcgtac gaatgagatt
     1561 ggaatccgca tggcgctcgg cgcgcgcggc ggcgatgtga tccgcctgat cggcgcgagc
     1621 ggcgtgcggc tcgcgcttgc gggaacgctg ctgggcgcgg cgggcgcatt cgccttgacc
     1681 cggtttttga gcggcatgtt gttcggcgtg agctcgctcg atcccgtgac gtttctggcc
     1741 atggcgggca cgttgatatt ggtgacgctg ctggcatgct atgtgcccgc ccggcgagcg
     1801 agcaaagtgg atcccctgat tgcgttgcgc tacgaataga aactgcgacg agggctcgac
     1861 gactccctta cggtcgcggc tctgacgggt gtcgcgtttg ccgaacatag ggaatccgca
     1921 tcagagacgt agatcgtgtg ctgattgaca atagctctat gcccgacgcc gtgtgctatc
     1981 actgcaaaca gacgctcacc cccggggagc cgcacgactg ttggacgacc accgaggctg
     2041 ccctcacgaa gggtctctcc gaagagctgc tcgacgcttg ggaacgcttg cgtgagacgg
     2101 ccgcggagtt tggcgaacag cgcatttacg catcccacaa atcgatcatg ttctcgcgca
     2161 agagctgcta ctttttcgtg cgccctaaga agaagatgct cgaaatctgc gtcttcctcg
     2221 atcgcgaagt gaaagcgcct cagatccggc gcgcggaccg cgtgtccaaa accaaggtgt
     2281 cgcacagcat cccgatcact caccgtgacc aggtcgaaag cccggtcacg gactggctgc
     2341 gcgaagccta cgacctctct caatagctac ttggtcgccg ttaccggcag ttcccacagg
     2401 aacttatgca ggaacttctc catacgatca agcgtgtata gccagcggct gtgcaacagc
     2461 gattcgtgca cgtcgtcggg gaatacgatc agttcgaagt acacatcgcg ttggcgcagt
     2521 aactgcacca ggccggtcat ctgctggaac gcgacgttgc gatcgtcatc gccctgaatc
     2581 agcagcaccg gcgatttcca accatcgatc gcgccaaccg cagaggactg gtaggagagc
     2641 gccgcgggat cgagcgaact gccatacaga tggacgcccg ccagatcgac gccggccttg
     2701 aagatatcgg aattgcgcgc gagggcctgc gcggtcagca ggccgccata cgagagaccc
     2761 cagatgccga cccggttggg gtcgacatcg gcgcgggtct gcaggtattt gccgccggcc
     2821 agcacgtcct gatattcgct attgcccgcc gcattggtct tcggagcgtt gcggaacgag
     2881 cggccgtagc ccacgccgct gcggtagttc accgacagca cgacgtaccc ctgattcgcc
     2941 agccactggt tgatgccgta cgcccagtgg tagaactgca tgtaatgata ggcggccatc
     3001 atctgccgct gcggaccgcc gtgcacgaag acgatggcgg gacgtcgctc gccgggcttc
     3061 aggtccttcg gcaggaagag ttggttgtga atctccatgc cgtcggcggc cttcgtgagg
     3121 acgatttcgg gcttcacgtg cgcttcggcc ggaaagcctg cgagcgtggt cggaaaaatt
     3181 attttttggg tggccggggc tcccgccgca atgttccaga tgcccagcga ttgcggcata
     3241 ttccacgccg cgctcagggt cgcaaggtat ttgccggaag cgagcggcgc gggagatgtt
     3301 tcgaccgctt cgcccgtggt gatctgggcg ggcttgccgc cgctcacggg tacggaccat
     3361 atatggcggc gctcgatgtc ctgggcattg gtgcagtagt agaacgtctt gccgtcggcg
     3421 gagagcgcga tcgaagtctg gttttcgatc atgccgtcgg tggtggtgag cagcacgggg
     3481 cgggcagccg cgtcggtcac gtggatcgaa tagtagcgtt cccactcgtc agcgggcgtc
     3541 tcaggtgttt gtggcgtggc agcagcggcg gccccacgac ctccgccgcg acctccgaag
     3601 cccgcgccga attggaaaat caggtactcg cccgcaaggc gggcccccgt gaggttggtc
     3661 accaacggat cgttagcctg gttgtgccag atctcttgcg cgtcaccggt ggacgcgtcg
     3721 cctttgtaga ctgcgatggt gtaaccgccg cggaaggtcg cgcgcatcag gccggccgaa
     3781 gtattggcag gcgctgccgg ggtag
//
LOCUS       DATIMH010000424         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_99165, whole genome shotgun sequence.
ACCESSION   DATIMH010000424 DATIMH010000000
VERSION     DATIMH010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99165"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VLI90_07265"
     CDS             <1..209
                     /locus_tag="VLI90_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14042.1"
                     /translation="FGWMRDSNAYFLGLPFWMLLPAAAGLSIWLWRARPPKHAAGVCR
                     RCGYDLRATPDRCPECGAAVTPAD"
     gene            346..1542
                     /locus_tag="VLI90_07270"
     CDS             346..1542
                     /locus_tag="VLI90_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14043.1"
                     /translation="MTTPLAARGDDLFSLIATSSSTTARGAGSNVIDLVKNLTNNADQ
                     FAALGGQSFTSTLNYAGIANAVKVNQSFDPQGHRIINLQVPSVGLNKTFNEANGNLTD
                     QVRDFLKTGGLAALGEFQAVVNRESLAGIVDGNPLAATAMLTDAGYEQFALHRSTQNL
                     LGEPYTVATSGHGETRLSADGGVFDAGGFTGTFVDFTLASEVNFNDRIGLAASAPVRW
                     LDFHGANVFMGGLILGLPITILPGHGDRSLQWQITPAGHAAAVGSADFASGGILYGGQ
                     IDNSLSYNFGDGFSITLGTTAGYFHGADISVAGYDFDTKLNQWVFKNGVLLSKAWDNF
                     FIDGGASWTNFLRDAYTDGYLTPELGLGWRFGRGNNSGLRIGYTGHFGNHYNTNGGSV
                     LLFFTG"
     gene            complement(1628..>2170)
                     /locus_tag="VLI90_07275"
     CDS             complement(1628..>2170)
                     /locus_tag="VLI90_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14044.1"
                     /translation="TCDSTQPGTTLIGGDGNDTLIGGDAPDKLFGGNGNDRLVGNAGS
                     DTMDGGAGSDTMIGGAGTDVADYHTRTANLVVWLDGSHPSGQTGENDHFDGTVEIVWG
                     GSGNDYLVGTKSNNALYGNGGNDTLYGGGGVDAFFGGSGNDQIHAKNGNKDYVDGGGG
                     NDSAITDPIDTVVNVETVSH"
BASE COUNT          348 a          733 c          665 g          424 t
ORIGIN      
        1 gctttggttg gatgcgggac agcaacgcat atttcctggg acttccgttc tggatgctgc
       61 tgccagctgc ggcggggttg tcgatttggc tatggcgcgc ccgcccgccg aagcatgctg
      121 ccggcgtttg ccggcgttgc gggtatgacc tgcgcgccac gcccgaccgc tgccccgaat
      181 gtggagcagc cgtcacgccg gcggattaat tccagatctg ccacatactg ctagtcgttt
      241 cagccttcgc gcacgcggcg tccccgctta ctttctcaag cattccgcac aatggaggcc
      301 ttgaaaatgc catgccgatt tgccctcgct ttgtttctca tcgcggtgac aacgcccctc
      361 gccgcgcgcg gcgatgatct gttctcgctc attgccacca gcagctctac caccgcgcgc
      421 ggcgcgggat ctaatgtcat cgacctggtg aagaacctga ccaacaacgc cgaccagttc
      481 gcggcgctcg gcgggcagag cttcacctcg acgctcaact acgccggtat tgccaacgcg
      541 gtgaaggtca accagagctt cgatccccag ggccatcgca tcatcaacct gcaggtgccc
      601 agcgtcggct tgaacaagac gttcaacgaa gccaacggca acctgaccga tcaggtgcgg
      661 gacttcctca agaccggcgg gctggcggcg ctcggcgaat ttcaagccgt cgtcaatcgc
      721 gaatcgctgg ccggcatcgt cgatggaaat ccgctggctg ccaccgccat gctgaccgat
      781 gcgggctacg agcagttcgc gctgcaccgc agcacgcaga acctgctcgg cgagccgtat
      841 accgtcgcga cgtccggtca cggtgaaacg cggctcagcg ccgacggcgg cgtcttcgac
      901 gccggggggt tcaccggaac cttcgtcgat ttcaccctcg cttccgaagt gaacttcaac
      961 gaccgcatcg gcctcgccgc cagcgcgccc gttcgttggc tcgacttcca cggcgccaac
     1021 gtctttatgg gtggcttgat cctcggcctg ccgatcacca tcctgcccgg tcacggcgat
     1081 cgcagcctgc aatggcagat cacgccagcc ggtcacgccg ccgccgtcgg ttccgccgac
     1141 ttcgccagcg gcggcatcct ctacggcggg cagatcgaca actcgctgtc gtacaacttc
     1201 ggcgacggct tctcgatcac gctcggcacc accgcggggt acttccacgg cgccgacatc
     1261 tccgtcgccg gttacgattt cgacacgaag ctgaaccaat gggtcttcaa gaacggcgtg
     1321 ctgctgagca aggcgtggga caatttcttc atcgacggcg gggcgagttg gaccaacttc
     1381 ctccgcgacg cgtacaccga cggctatctc acgcccgagc tgggactggg ctggcgcttc
     1441 ggccgcggca acaactccgg cctgcgcatc ggttacaccg gtcacttcgg caaccactac
     1501 aacaccaacg gcggcagcgt gctgctgttc ttcacggggt gaacgagccc cctgtaaagc
     1561 cgccgccagt ggcggcggga ggccgcggtc cttgcacccc gaatgacgag gcgccccgcg
     1621 gggactttca gtggcttacc gtttccacgt tgacgaccgt gtcaatcgga tcggtgatcg
     1681 ccgagtcatt cccaccgccg ccgtcgacgt agtccttgtt cccgttcttc gcgtggattt
     1741 ggtcgttgcc tgagccacca aagaacgcat caacgccacc gccgccgtag agggtgtcat
     1801 tgccgccgtt gccgtagagg gcgttgttgc ttttagtacc gacgagatag tcgttgcctg
     1861 atccgcccca gacgatttcg acggtgccgt cgaagtggtc gttttcgccg gtttggccgc
     1921 tcgggtggga accgtcgagc cagacgacga ggttggcggt gcgggtgtga tagtccgcca
     1981 cgtccgtgcc cgcgccgccg atcatcgtgt cgctgccggc gccgccgtcc atcgtgtcgc
     2041 tgccggcgtt gccgacgagg cggtcgttgc cgttgccgcc gaagagtttg tcgggcgcgt
     2101 cgccgccgat cagcgtgtcg ttgccgtcgc cgccgatcag cgtggtgccg ggttgggtgc
     2161 tgtcacaggt
//
LOCUS       DATIMH010000425         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5299170, whole genome shotgun sequence.
ACCESSION   DATIMH010000425 DATIMH010000000
VERSION     DATIMH010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5299170"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VLI90_07280"
     CDS             <1..865
                     /locus_tag="VLI90_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV14045.1"
                     /translation="QPELVIKPRRGWIAVDWRELWNHRELLYFLVWRDVKVRYKQSVL
                     GVAWAVLVPVLNMLVATLLFGVVFHLGKGIVGPNGKPSPYAILVFAALLPWQLFSNSV
                     SQGGLSLLNQQHLLTKIYFPRLFIPTAAVGGLLVDMAISFGVLIALMTIYHFVPTYQF
                     VPYWGIVFVPFLTMLTVISGLGAAYLLSALTVTYRDFRFLIPFAVQILSLMSFVQFPA
                     ETVVAGHPKLSWVLSINPMFGVVAGFRSAILGQPFHLGYLLVSIVVSVTMLVFGMFYF
                     RKTERRFADIA"
     gene            929..2224
                     /locus_tag="VLI90_07285"
     CDS             929..2224
                     /locus_tag="VLI90_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV14046.1"
                     /translation="MSTPIITAEGLGKAYRLGLKVDKHQTFRDAIMGAFTSPLQKLKA
                     RIGEPTGGEDTFWAIRDISFDVNRGEVIGLIGRNGAGKSTLLKVLSRITEPTEGKATL
                     RGRVASLLEVGTGFHPELTGRENIYLNGAILGMKKAEIDSKFDQIVEFAEVSKFLDTQ
                     VKRYSSGMYVRLAFAVAAHLEPEILIVDEVLAVGDASFQKKCLGKMSDVVKDGRTILF
                     VSHNMDAIASLCTHTLLVQGGRVGERLTSDEGIKRYMALSNEGSHLPLRDKPRIQSKK
                     RPPVFVGISIQGSSGHKNVVESGGGVTFEVEIANCEDLKSGGMCGIAITNDRGQRIAF
                     FHTLYQAGFTFSGSSHAKLTCHVPNLPLVPNSYFIELVLADGYGIIEKVERADRLDVV
                     YADVLGTGKIPGSRQGYVVWPSEWDYTGEMQQASEMATV"
     gene            2363..3673
                     /locus_tag="VLI90_07290"
     CDS             2363..3673
                     /locus_tag="VLI90_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV14047.1"
                     /translation="MRILHLSTSDTSGGAARAAYRLHTGLRRLGHDSTMLVLKRTSGD
                     PSVNGLRWSTDFATRFRRRRRRKQIARNFEPYKRTLPAGFEIFSDDRSEAGFDLARQL
                     PPCDLINLHWVAGLIDYELFFDNLPKGVPLVWRLADMGALTGGCHYDGGCGKFTAACG
                     ACPVLGSNVEHDLSRDVWTRKNAALSNLPDDDLHLVGTTRWIAGEARRSSLLGRFPVT
                     VIPNGLDTDDFAPRDKRFSRDTLGVPRDAKVVLFAAESSNNKRKGFDYVAAALAGMAD
                     EPNLFLLSIGGGDPQVANVPHLHLGRITNDRMLSVIYSAADVFAMPSLQESFGQTITE
                     SMACGTPVVAFNTGGIPDLVRPGITGQLAAAGDAAALRDSMRQILRNPASWPQLSANC
                     RRIAIEEYSLQTQAQRYAELYQTILSPRPTANREPVATMVEISR"
BASE COUNT          686 a         1169 c         1189 g          703 t
ORIGIN      
        1 gcagccggag ctggtgatta agccgcgccg cggctggatc gccgtggact ggcgcgagct
       61 gtggaaccat cgcgagctgc tgtacttcct ggtctggcgc gacgtgaagg tgcgctacaa
      121 gcagtcggtg ctgggcgtcg cctgggcggt gctggtgccg gtgctgaaca tgctcgtcgc
      181 gacgttgctg ttcggcgtgg tgttccacct ggggaagggc atcgtcggcc ccaatggcaa
      241 gccgtcgcca tatgcgatcc ttgtcttcgc ggccttgctg ccgtggcagc tgttttccaa
      301 cagcgtcagc caaggcgggc tttcgctgct caaccagcaa catttgctga cgaagattta
      361 ctttccgcga ttgttcatcc ccaccgccgc cgtcggcggg ctgttagtgg atatggcgat
      421 ctcgtttggc gtgctcatcg cgctgatgac gatttaccat ttcgtgccga cataccagtt
      481 cgtgccgtac tggggcatcg tcttcgtgcc gttcctgacg atgctgaccg tcatctccgg
      541 cctcggagcg gcgtatttgc tttcggctct gaccgtcacc taccgcgact tccgattcct
      601 gattccattc gcggtgcaga tcctcagcct gatgagcttc gtgcagttcc ccgcggaaac
      661 ggtcgtcgcc ggtcatccaa aactgagttg ggtcttgagc atcaacccga tgttcggcgt
      721 ggtcgcgggc ttccgttccg ccatcctggg gcagccgttc cacctcggct acctgctggt
      781 gtcgatcgtc gtcagcgtga cgatgctggt cttcggcatg ttctacttcc gcaaaaccga
      841 acgccgcttc gcagatatcg catgagaaag tggtcacggt tcaggggtct ttctgatcct
      901 gaaccctgaa ccccgaacac tgaaccctat gagtaccccg atcatcaccg ccgaaggctt
      961 gggcaaggct tatcgcctgg gtctgaaggt tgataagcat cagacgtttc gcgatgcgat
     1021 catgggcgcg ttcacgtcgc cgttgcagaa gctgaaggcc cgcatcggtg aacccaccgg
     1081 cggcgaggac acgttctggg cgattcgcga catttcgttc gacgtcaatc gaggcgaagt
     1141 gatcggcctg atcggccgca acggcgcggg caagagcacg ctgctgaaag tcctgtcgcg
     1201 catcaccgag cccaccgaag gcaaggcaac gctgcgtggc cgagtggcgt cgctgctgga
     1261 ggtcggcacc gggttccatc cggaactgac cggccgcgag aacatctatc tcaacggcgc
     1321 gatcctcggc atgaagaagg cggagatcga cagcaagttc gatcagatcg tcgaattcgc
     1381 cgaggtgagc aagttcctcg acacgcaggt caagcgatac agcagcggca tgtacgtgcg
     1441 cctggccttc gcggtggcgg cgcacctgga gccggagatt ttgatcgtcg atgaggtgct
     1501 cgccgtcggc gacgcctcgt tccagaaaaa gtgcctgggc aagatgagcg acgtggtgaa
     1561 ggacgggcgg acgatcctgt tcgtcagcca caacatggac gcgatcgcca gcctgtgcac
     1621 gcacacgctg ctggtgcagg gcggccgggt gggtgagcgg ttgacctccg atgaaggcat
     1681 caagcgttac atggcgctga gcaacgaagg ctcgcacctg ccgctgcgcg acaagccgcg
     1741 cattcaaagc aaaaagcgcc cgccggtttt cgtcggcatc tcgatccagg gatcgagcgg
     1801 tcacaagaac gtcgtcgaaa gcggcggggg cgtgacgttc gaggtcgaaa tcgccaactg
     1861 cgaagacctg aaatccggcg ggatgtgcgg catcgcgatc accaacgacc gcgggcagcg
     1921 catcgcattc ttccacacgc tgtatcaggc gggcttcacg ttcagcggtt ccagtcacgc
     1981 gaagctgacc tgccacgtgc cgaacttgcc gctggtgccc aacagctatt tcatcgagct
     2041 ggtgctggcg gacggttacg gcatcatcga aaaggtcgag cgggccgatc gcctggacgt
     2101 cgtctacgcc gacgtgctcg gcaccggcaa gatccccggc agccgccagg gttacgtcgt
     2161 ctggcccagc gagtgggatt acaccggcga aatgcaacag gccagcgaaa tggcgacggt
     2221 ttagcatttc cccctctccc acgtactcgt gggagagggc tggggtgagg gtgatttcga
     2281 acgtcgagtg attctcgaat tctccaatca ccctcaccct aaccctctcc cggagtaccg
     2341 ggcgagggga ccagaaacgc agatgcgcat cctccacctc agcaccagcg ataccagcgg
     2401 cggggcggcg cgggcggctt atcgcctgca caccggcctt cgccggttgg ggcatgactc
     2461 gacgatgctc gtgctgaagc gcaccagcgg cgacccgagc gtgaacggcc tgcggtggtc
     2521 caccgacttc gccacgcgct ttcgccgcag gcgccggcgg aaacagatcg cgcggaattt
     2581 cgagccgtac aagcgcacgc tgcccgcggg ctttgagatc ttcagcgacg atcgcagcga
     2641 agccggcttc gatctcgccc ggcagttgcc gccgtgcgat ctgatcaacc tgcactgggt
     2701 ggcggggctg atcgattacg aattgttctt tgacaaccta cccaagggcg tgccgctggt
     2761 ctggcggctg gcggacatgg gcgcgctgac cggcggctgc cactacgatg gcggctgcgg
     2821 gaaattcacc gccgcctgcg gcgcgtgtcc ggtgctcggt tccaacgtcg aacacgatct
     2881 gtcgcgcgac gtgtggacgc gcaagaacgc agcgctgtcg aacctgcccg atgacgacct
     2941 gcacctggtc ggcacgacga gatggatcgc cggcgaagcc aggcgaagtt cgctgctcgg
     3001 gcggttcccc gtgacggtta ttcccaacgg cctcgacacc gacgacttcg ccccacgcga
     3061 caagcgcttc tcgcgcgaca cgcttggcgt gccgcgtgac gcgaaggttg tgctcttcgc
     3121 cgccgagtcg tccaacaaca agcgcaaggg tttcgactac gtcgccgcgg cgctggccgg
     3181 gatggcggat gaaccgaacc tgtttctcct gtcgatcggc ggcggtgacc cgcaggtggc
     3241 taacgtcccg cacttgcacc tgggccgcat caccaacgat cgcatgttgt cggtcatcta
     3301 tagcgcggcc gacgtgttcg cgatgccgtc gctgcaggag tcattcggcc agacgatcac
     3361 cgagtcgatg gcgtgcggca caccggtcgt cgcgttcaac accggcggca tcccggacct
     3421 ggtccgcccg ggcatcaccg gccagttagc agcagccggc gatgccgcgg cgctgcgcga
     3481 ttcgatgcgc cagatcctgc gcaacccagc cagctggccg cagttaagcg ccaactgccg
     3541 gcggattgca atcgaggagt actcgctgca gacgcaggcc cagcgatacg cggagcttta
     3601 tcagacgatc ctgtccccac ggccaactgc aaaccgggag ccggtcgcaa ctatggttga
     3661 aatcagtcga taatatcaga gcattgaatt ctcttttggg acagacaaat gcgaccgcga
     3721 aggcgcgaag ccgcgaagga agacgcg
//
LOCUS       DATIMH010000426         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4722547, whole genome shotgun sequence.
ACCESSION   DATIMH010000426 DATIMH010000000
VERSION     DATIMH010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4722547"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VLI90_07295"
     CDS             <1..345
                     /locus_tag="VLI90_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV14048.1"
                     /translation="NLMINAVDAMANVAEPKLIVRTRRAGRWYTIDVIDNGSGIAPEH
                     VNRLFEPFFTTKPIGKGTGLGLSISYSLMRKQGGDIQVQSKPGEGAMFTVRVPVRETR
                     AGTPCAPAAALA"
     gene            530..1048
                     /locus_tag="VLI90_07300"
     CDS             530..1048
                     /locus_tag="VLI90_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSV14049.1"
                     /translation="MTFLGCRLIGTAAIWFAAGLAGCGKSDGGGGGQPAPHVPVKVAS
                     AAEATAETFAVDPPDAEVGQQVFIQTCATCHGFQAQGLPHQGAPLRTSPFIASHDDQA
                     LIEFIKQGRPAKDPSNASGVAMPPRGNNPAISDARLADVVAFLRQVQAEAKAESQQTM
                     VAPSASPTTVAP"
     gene            1064..>2497
                     /locus_tag="VLI90_07305"
     CDS             1064..>2497
                     /locus_tag="VLI90_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14050.1"
                     /translation="MSTIREDGMSIIHEPAPARATCVRRTKILIALAAVIVVAGATLP
                     IAIRGGGIKTRIVRLFKPEYGLSKPVVRFRSHPINGGRDVSANTAISISIVVRNGKLD
                     AKTMNTAHVALQPAGGGAALDVAVRPVGDDLLVLRPKAALQPDTAYTLSISGMKSTIG
                     AMIPAFTTRFTTGRPADADIRFQKVPLPGTAGYACTAVVMGPDHQLYAGTDDGVILRF
                     PIQPNGTLGEPLKIKSLQTAEGRSRLLIGFCFDPAATAAKPIVWVSHGFYAFSDAPDF
                     TGKITRLSGPKLEVVEDAVIHLPRSARDHLNNQPIFGPDGALYFGQGSNTSYGAPDPI
                     WSNRGQHLLNAAILRLDVKRITPGQPLDARTVDADGSYDPRSPDAPLTAYATGVRQAY
                     DLVWATNGQLYAAINGSSAGGNTPAGHGAPALIDIPDDENDWLFHVMPGRYYGHPNAL
                     WNHFVLNGGNPDGGSGSSIVPQYPLGVK"
BASE COUNT          467 a          748 c          848 g          434 t
ORIGIN      
        1 aatctcatga tcaacgcggt ggatgcgatg gcgaatgtcg ccgagccgaa gctgatcgtg
       61 cgcacgcgcc gcgcggggcg gtggtacacg attgacgtga tcgacaacgg cagcggcatc
      121 gcgccggagc acgtgaaccg gctgttcgag ccgttcttca cgaccaagcc gatagggaaa
      181 ggtacgggac ttggtctatc gatcagctac agcctgatgc gcaagcaggg cggggacatc
      241 caggtgcaaa gcaaaccggg cgagggagcg atgttcaccg tgcgcgtgcc ggtgcgcgag
      301 acgcgggcgg ggacgccatg cgcgccggcg gctgcgctcg cgtgaattct tgaacacgcg
      361 accgttcaag agactatcct atccgctgaa tttgccggcc gttgcggcat ccagcgctta
      421 tcggacggcg attgtcataa tgttatcgca cgcgatcgtc cttgtccgcc ggcttgttgc
      481 ggtcgataat gaggataaga aagtccgttg aagtgtaagt gaggttgcaa tgacgtttct
      541 tgggtgccgt cttattggaa ctgccgccat ctggtttgcc gccgggttgg ccggctgcgg
      601 aaagagcgat ggcggtggtg gcggccagcc ggcgccgcac gtgccggtga aggtcgcgtc
      661 ggcggcggag gcgacggcgg agacgttcgc ggtggatcca ccggatgcgg aggtgggtca
      721 gcaggtgttc atccagacct gcgcgacttg ccatggcttc caggcccagg gcctgccgca
      781 ccagggggcg ccgctgcgca ccagcccgtt catcgccagt catgatgatc aggcgctgat
      841 cgagttcatc aagcagggca ggcccgctaa agatccatcg aacgccagcg gtgtggcgat
      901 gccgccgcgg gggaataacc cggccatcag cgatgcgcgg ctggccgatg tcgtcgcgtt
      961 cctgcggcaa gtgcaggcgg aggcgaaggc ggaatcgcag cagaccatgg ttgccccgag
     1021 cgcttcgccc accacggtgg cgccgtaaca acacgcggaa aacatgagca caatcaggga
     1081 agatggcatg tcgatcattc acgaaccggc gccagcgcgc gcgacgtgcg tgcggcgaac
     1141 gaaaattctg atcgcactgg ctgcggtgat cgtcgtcgcc ggcgcgacac tgccgatcgc
     1201 gatccggggc gggggcatca agacgcgcat cgttcggctg ttcaagccag agtacggcct
     1261 gagcaaaccc gttgtccgct tccgctcgca tccgatcaac ggcggacgcg atgtgagcgc
     1321 caataccgcc atctcgatct cgatcgtcgt ccgcaacggc aagctcgatg cgaagacgat
     1381 gaacacggcc cacgtagcgc tgcaaccggc gggcggtggg gcagcgctgg atgtggcggt
     1441 tagacccgtg ggcgacgatc tgctcgtcct gcgtccgaag gctgcgcttc agccggacac
     1501 ggcgtacacg ctgtcgatct ccggtatgaa gtccaccatc ggcgcgatga tccccgcgtt
     1561 cacgacgcgc ttcacgacgg gtcgcccggc tgacgccgac attcgatttc aaaaagtgcc
     1621 gctacccggc accgcgggct acgcctgcac cgcggtggtc atggggccag accatcagct
     1681 gtacgcgggg actgacgacg gagtgatcct gcgctttccc attcagccta atgggacgct
     1741 gggtgagccg ctgaagatca aatcgctgca aacagccgag ggtcgatcgc gattgctgat
     1801 cggcttctgc ttcgaccccg ccgcgacggc ggcgaagccg atcgtctggg tatcgcatgg
     1861 cttttacgcg ttttcggatg cgccggattt caccggcaag atcacgcgat tgagcgggcc
     1921 gaaattggaa gtcgtggaag acgcggtgat tcacctgccg cggtcggcgc gcgatcatct
     1981 gaacaatcag ccgatcttcg gacccgacgg tgcgctgtat ttcgggcagg ggagcaatac
     2041 gtcctatgga gcgcccgacc cgatctggag caaccgaggg caacacctat tgaacgcggc
     2101 gattctgcgg ctggatgtga aacggatcac gccggggcag ccgttggatg cgcgcacggt
     2161 ggatgcggac ggttcgtacg atccgcgatc gcccgatgcg ccgctgacgg cgtacgccac
     2221 cggcgttcgc caggcgtatg acctggtctg ggcgaccaac gggcaattat acgcagccat
     2281 caacggctcc agcgccggtg gcaatacgcc cgcggggcac ggggcgccgg cgctgattga
     2341 cataccggac gacgagaacg attggctctt tcacgtgatg ccgggccgat actacgggca
     2401 tcccaacgcg ctgtggaacc attttgtgtt gaatggcggc aaccccgacg gcggcagcgg
     2461 ttcgtccatc gtgccgcagt atccgctggg cgtgaag
//
LOCUS       DATIMH010000427         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2408424, whole genome shotgun sequence.
ACCESSION   DATIMH010000427 DATIMH010000000
VERSION     DATIMH010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2408424"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1250)
                     /locus_tag="VLI90_07310"
     CDS             complement(12..1250)
                     /locus_tag="VLI90_07310"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSV14051.1"
                     /translation="MIDISILYGGLETPSTPHRYGRAITQIDPPAHQRMAVAKSAAQR
                     RPIVVWNITRTCNLKCVHCYTDSDAKKYPDELSTKQAKAVLDDLADFNVPAVLFSGGE
                     PLVRKDLFELATYARSLGLHVVASTNGTLIDRPVAQRFKDLQFSYVGVSLDSAIPAVH
                     DEFRGQKGAFERTMQGFRHCVAVGQKVGLRLTLTRHTAGDLDGIFDFIEREGIDRACF
                     YHLCPAGRGKSLYALTSEDTRKAVDKIFDRTKDMLDRGKRVEILTVDNHADGPYLYMR
                     MKREGHPRAQQVHEMLTWNGGGRYSSGVGIANIDFYGNVHADQFSMFRRFGNVKQRKF
                     SEIWQDTSDPIMAGLKDRLPLLKGKCATCRFKNQCGGSLRARAEVTTDDPWAPDPGCY
                     LTDEEIAAEAPAELAAAALA"
     gene            1648..2556
                     /locus_tag="VLI90_07315"
     CDS             1648..2556
                     /locus_tag="VLI90_07315"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSV14052.1"
                     /translation="MFQTLAVICLTLLISTQSRATENPHWRKDACESCHAKRGEQLQP
                     IAPAAITETCLKCHDGAKSVAEVHPIARPVVKDGYTDPGWPTAAGNLDCVTCHDIKQA
                     CQTSGETPEVNSAFLRGDHDGSPSSFCANCHKSERVRKFNPHIMLSQDRQVMEDRCQV
                     CHGKIPDRNTLARSGQTTLKEDQLVLCRSCHPHHKDISRAGHVGTTVKPEMLAYMRAR
                     ELSGLLTIPDKSVVNDLMAAGAKPTLMVPNPDGTVVCSTCHNPHERGVFPATSVLAYH
                     AMKVVDGHTRTPVRGEQFCNHCHNGF"
     gene            2756..>3639
                     /locus_tag="VLI90_07320"
     CDS             2756..>3639
                     /locus_tag="VLI90_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14053.1"
                     /translation="MTRASGPSAQRPHAAASQSSSRLPSRLRAFALAFLFLLTAALAS
                     TAHAADTEGCLTCHQYRGLSRIGPDGTSISLFYVDPNYFSLALGPHARLKCTDCHTRS
                     EVEVFPHKPPIAVDCGKTCHLIAPGKVEVRFSHDRVDGMLDKSIHKGNVLDEANALLG
                     SPLKPDQSRCLLCHDEPTFRRSGQNWVQQETPIQRCNACHDEQMPADTRHFYWHVEAR
                     SKPARDNADIVRVCAQCHSNPAIQAKFKLPDVTTSYLQSFHGKATQLGSQETANCIDC
                     HVGQMQNVHVMKAHSDPSS"
BASE COUNT          679 a         1193 c         1114 g          653 t
ORIGIN      
        1 gtgctacaga cctacgccaa cgcggccgcg gcaagttcgg ctggcgcctc ggcggcaatt
       61 tcttcatccg tcaaatagca gcctggatcc ggcgcccacg ggtcgtccgt ggtcacttcc
      121 gcccgcgccc gcaatgatcc gccgcactga ttcttaaacc ggcaggtcgc gcatttcccc
      181 ttcagcagcg gcagacggtc cttcaatccc gccatgatcg gatcgctcgt gtcctgccag
      241 atctcgctga actttcgctg tttcacgttg ccgaacctgc ggaacatcga gaactgatcc
      301 gcgtgcacgt tgccgtagaa atcgatgttg gcgatgccga cgccgctgga atatcgcccg
      361 ccgccgttcc aggtgagcat ctcatgcacc tgctgggcgc gcgggtggcc ctcgcgcttc
      421 atccgcatgt acaggtacgg cccatcggcg tggttatcga cggtcaggat ctccacgcgc
      481 ttgccgcggt cgagcatgtc cttggtgcga tcgaagattt tgtcgacggc cttgcgcgtg
      541 tcctcggagg tgagcgcata aagactcttg ccgcgaccgg ccgggcacag gtgatagaag
      601 caggcgcggt cgatgccttc gcgttcgatg aagtcgaaga tgccatcaag atcgccggcg
      661 gtgtggcgcg tcagcgtcaa tcgcaggccc accttctgtc cgacagccac gcaatggcgg
      721 aagccttgca tcgtccgttc gaacgcgccc ttctgcccgc ggaattcatc gtgcacggcg
      781 gggatcgcgc tgtcgaggct cacgccgacg tagctgaact gcaagtcttt gaaccgctgc
      841 gcgacggggc gatcgatcaa cgtgccgttg gtcgatgcca cgacgtgcag gcccaggctg
      901 cgcgcgtatg tcgccagttc gaataaatcc ttccgcacca gcggctcgcc gccgctgaac
      961 agcacggccg gtacgttgaa gtcggccaag tcatccagca cggccttggc ctgcttcgtg
     1021 ctcagctcat ccggatattt cttcgcatcg gaatcggtgt aacagtgcac gcacttgaga
     1081 ttgcaggtgc gcgtgatgtt ccacacgacg atcggcctgc gctgagcggc gctcttggca
     1141 accgccatgc gctgatgcgc cggcgggtcg atctgggtga tcgcgcgacc atagcggtgc
     1201 ggggtgctgg gcgtctcgag cccgccgtac aagatgctga tatcgatcat ggcgtgatcc
     1261 tgatcatgag ctccggggcc gttgccgcgc gcacgttccg cggtgcgcag gcgacacctg
     1321 ctgttgcttg aacaaagacc atgccggtgg cggatggcgg tttgcacgcc gccgcagggg
     1381 cttggtcgcg actggttctc agcttcggga aggcccggcg tgcttcatcc tcataatggg
     1441 agaacaaaga ccttttatcg ccccgcaaga cgcgtaaatg cgggtgcggc gatggtattg
     1501 cgcttgctcg ggctgtagcg ggcgcccatg ggtttgattc ttacgcagca tcgtcgtcga
     1561 cgccggctag cggcgcaaag cccacggctc tgccgtggga cgtttttcaa atcccctccc
     1621 atggcagagc cacgggcttt gggttttgtg ttccagaccc tcgcggtgat ctgcctcacg
     1681 ctgctcatct cgacacaatc gcgcgcgacc gaaaatccgc actggcgcaa agatgcctgc
     1741 gaatcctgcc acgccaagcg aggcgagcaa ctccaaccca ttgcccccgc cgccatcacc
     1801 gaaacgtgcc tgaagtgcca cgatggcgcc aagtcggttg ccgaggtgca cccgatcgct
     1861 cggccggtgg tgaaggatgg atataccgat ccgggttggc ccacggctgc cgggaatctc
     1921 gactgcgtca cctgccatga catcaagcaa gcgtgccaga cgagcggcga gacgccggag
     1981 gtcaacagcg cattcctccg cggcgaccat gacggctcgc cgtcttcctt ctgcgctaac
     2041 tgccacaaga gcgagcgggt gaggaagttc aacccgcaca tcatgctgag ccaggaccgg
     2101 caggtgatgg aagaccgctg ccaggtctgc cacggcaaga tcccggaccg aaatacgctg
     2161 gcgcgttcgg gccagacgac gctgaaagag gaccagctgg tcctttgccg cagctgtcat
     2221 ccgcatcaca aggacatctc gcgcgccggc cacgtcggca cgacggtcaa gccggagatg
     2281 ctggcgtaca tgcgagcccg cgagctgagc ggcctgctga ccatccccga caagtcggtg
     2341 gtgaacgatc tgatggccgc cggcgccaag ccgacgctga tggtgccgaa ccccgatggc
     2401 acagtcgtct gttcgacctg ccacaacccg cacgaacgcg gcgtgttccc ggcaaccagc
     2461 gtgctggcgt accacgcgat gaaggtggtg gacgggcaca cgagaacgcc ggtgcgcggc
     2521 gaacagtttt gcaatcactg tcacaacggt ttttgaaatg atcgcggtta gagccaactt
     2581 cgatgcttcc tccggcgacg aaggcgttgc gcgcgcggat tcaatgcgaa cgcgaagacg
     2641 cgaagacgcg aaggaagacg cgaagcggaa ttgggtggat ggattaattg acgttcgagc
     2701 gccgctcgca cgggccggag gcccgtgtca cagtgaaggt tcaaatggcg ccaccgtgac
     2761 ccgggcctcc ggcccgagcg cgcagcgtcc gcacgccgca gcctcccaat cctcttcgcg
     2821 tctgccttcg cgtcttcgcg ccttcgcgct tgcgttcctc ttcctcctca ccgcagcgct
     2881 cgcgagcaca gcccacgccg ccgataccga aggctgctta acctgccacc agtaccgcgg
     2941 cctctcgcgc atcggtcctg acggaacatc gatctcgctc ttctacgtcg atccgaacta
     3001 cttcagcctt gcgctcgggc cgcacgcgag gctcaagtgc accgactgcc acacgcgatc
     3061 ggaagtcgaa gtgtttccgc acaagccgcc gatagccgtg gattgcggca agacctgcca
     3121 tctcatcgcg cccggcaaag tcgaagtccg cttcagccac gaccgggtcg acggcatgct
     3181 cgacaagtcg atccacaagg gcaacgtact cgacgaggcg aatgcgctgc tcgggtcacc
     3241 gctgaagccg gatcaatcgc gctgcctgct gtgccacgat gagccgacgt tccgccggtc
     3301 tgggcagaac tgggtgcagc aggaaacgcc gatccagcgc tgcaatgcct gccacgacga
     3361 gcagatgccg gccgacacgc ggcatttcta ttggcacgtc gaggcgcgat ccaaacccgc
     3421 gcgcgacaac gccgacatcg tccgcgtctg cgcgcagtgc cacagcaacc cagccatcca
     3481 ggcgaaattc aagctgcccg acgtgacgac gagctacctg cagagcttcc acggcaaggc
     3541 cacgcagctg ggcagccagg agaccgccaa ctgcatcgat tgccacgtcg ggcagatgca
     3601 gaacgttcac gtgatgaagg cgcattcgga tccgtcatc
//
LOCUS       DATIMH010000428         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1544410, whole genome shotgun sequence.
ACCESSION   DATIMH010000428 DATIMH010000000
VERSION     DATIMH010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544410"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="VLI90_07325"
     CDS             <1..1880
                     /locus_tag="VLI90_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HSV14054.1"
                     /translation="ANFVVHNKGGCFPAGTMVDTPRGRVRIEQLRVGDAVITGSVSSA
                     AKSGSSEYLRTRRGGIVERSETTFGQSSVRATFSTRSSLLVLVTEAGELRVTPEHPLA
                     LVSREFRAAEEIAIGESLLVRQGDQLRATQLLSRRQEAQPVTVYNLEVDDPHTFIADG
                     FVVHNKGGFSGGHSSSHSYGGSSRYYGSSRSSTRPTSGPARVMWWFWDECGFFGCLVG
                     GIFAINAVLSLFKRKSHGDEDLDFVYSPAAVAAKAMKTRQLLATLAATDPSMDPGKLV
                     QLARDTFVKLQQCWESREYGPMQPLMMPDLYRQHVEQLDGMKRNHEINRIDNLQVDAI
                     DVVNVRYTYKENTREFTALISARARDFYVDDRNNHFLRGDAQPARFQEFWTFHRQDGQ
                     WLLREVEQSRESDVLKDENFVEQFTDTHLKQVYATAASPGGPAGPWLEKQVETKATKT
                     ERLLNFLVQTDKLWNRDAMLTRARDVFVAVMLAQESNDPTSVPADDMFPHAADDLRRV
                     IAQRRMNGGRIEYRNLCVRKVELILVRNFADNSKDEFVTRISAHAQTIITQNGRTTRA
                     DEYVTPFMQYWTFGRLDEQWKLKEVLAEMAGKAATTAENVDEDSSAEQMQWYYSKTRA
                     N"
BASE COUNT          408 a          590 c          688 g          346 t
ORIGIN      
        1 cggcgaattt cgtcgtgcat aacaaaggtg ggtgtttccc cgcggggacg atggtggaca
       61 cgccgcgagg gcgcgtgcgg atcgagcagt tgcgagtggg cgacgctgta atcacgggat
      121 ccgtttcgag cgccgcgaaa tccgggtcgt cggagtacct ccgaacccgg cgtgggggca
      181 tcgtcgagcg ttcggaaaca acctttggcc aatcttcggt gcgggcgacg ttctccacgc
      241 gatcgtcgct gcttgttctt gtgaccgagg cgggtgaact gcgagtcacg ccggaacacc
      301 cgctggcgct ggtgagccgg gaatttcgag cggccgagga aatcgctatc ggcgaatcgc
      361 ttctggttcg ccagggtgat caactgcgcg caacgcagtt actgtcgcgt cggcaggaag
      421 cgcagccggt gactgtctac aacctcgaag tcgatgaccc gcacaccttc atcgccgacg
      481 gttttgtggt tcacaacaaa ggcggcttca gcggtgggca ctcttcgagc cattcttatg
      541 gcggcagctc gcgttactac ggcagcagcc gcagttccac ccggccgacg agcgggccgg
      601 cgcgggtgat gtggtggttc tgggatgaat gcggtttctt cggctgtctg gtcggcggga
      661 ttttcgcgat caatgcggtg ctcagcctct tcaagcgcaa gagtcatggc gatgaagacc
      721 tcgacttcgt ctactccccc gccgccgtgg cggccaaggc aatgaaaacg cggcagctgc
      781 tggcgacgct cgccgccacg gatccttcga tggatccggg caagctcgtt cagctggcgc
      841 gcgacacgtt cgtgaagctt cagcaatgct gggaatcgcg cgagtacggc ccgatgcagc
      901 cgctgatgat gccggacctg tatcgccagc acgtcgagca gctggacggc atgaagcgca
      961 accacgagat caatcgcatc gacaacctgc aggtggacgc gatcgacgtc gtcaacgttc
     1021 gctatacata taaggaaaac acccgcgagt tcactgcgct gatcagcgcg cgggcgcgcg
     1081 acttttacgt cgatgaccgc aacaaccatt tcctgcgcgg cgacgcgcaa ccggcgcggt
     1141 tccaggaatt ctggaccttc catcgccagg acgggcaatg gctgctgcgc gaggtcgaac
     1201 agagccgcga gtcggacgtg ctgaaggacg agaatttcgt cgagcagttc accgacacgc
     1261 acctgaagca ggtgtacgcc accgcggcgt cgcccggtgg tccggcgggg ccgtggttgg
     1321 agaagcaggt cgagaccaag gcgaccaaga ccgagcggct gctgaatttt ttggtgcaga
     1381 ccgacaagct gtggaatcgc gacgcgatgc tgacgcgggc gcgggatgtc ttcgtcgcgg
     1441 tgatgttggc gcaggagtcg aacgatccga cgagcgtgcc ggccgacgac atgttcccgc
     1501 acgccgccga cgacctgcgg cgggtgatcg cccagcgccg gatgaacggc gggcgcatcg
     1561 agtaccgcaa cctgtgcgtg cggaaggtgg agctgatcct ggtccgcaat ttcgcggaca
     1621 acagcaagga cgaattcgtc acgcggatca gcgcgcacgc gcagacgatc atcacgcaga
     1681 atggacgaac gacgcgggcg gatgaatacg tgacgccgtt catgcagtac tggacgttcg
     1741 gccggttgga cgagcagtgg aagctgaagg aagtgctggc ggagatggcc ggcaaagcgg
     1801 cgacgaccgc ggagaacgtg gacgaggaca gcagcgccga gcagatgcag tggtattaca
     1861 gcaagacgcg ggcgaattga gcgaatctcg aagttttgag atcatcggcc gattcccgat
     1921 gccgaagaaa cacgccatcc ccaaagagtg gacggatcgc cttgaggcga tgcggcagtt
     1981 ccgcaccgcg gcagagctgg cattggcgca cggcccgccg ccgcacaagc aa
//
LOCUS       DATIMH010000429         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3276018, whole genome shotgun sequence.
ACCESSION   DATIMH010000429 DATIMH010000000
VERSION     DATIMH010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3276018"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..892)
                     /locus_tag="VLI90_07330"
     CDS             complement(11..892)
                     /locus_tag="VLI90_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSV14055.1"
                     /translation="MRLWLLAIPALLCAADLKIDHATVAGRDLKKMQAALATVGIPTI
                     YGGPHANGVTEMALASFADGSYLEAIAIQPKADPKAVDRHEWAQFLKEDGTPCAWAVQ
                     SKDLAGDRKRLKAAGVTVSEPVRAGRQRPDGVRLDWETADLGTAVRGSFFPFFIHDFT
                     PREQRAFPAGKPTTRDFKAVTRIVIAVRNLDAAVEEYRKAFALPAAIKQVDKDFGAQL
                     AIVGDAPVVFAQPLHAGTWLDERVQKFGDGPCAFILGATRPGPYKAASKSRWFGIDIS
                     WFDAEKLGWHLGYEAAR"
     gene            complement(938..>1574)
                     /locus_tag="VLI90_07335"
     CDS             complement(938..>1574)
                     /locus_tag="VLI90_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14056.1"
                     /translation="GGLSDAVYFNGKVYFANQAGGSISVLPVNPATGAAAGAITAVKV
                     DLGARALAIDTKDNLLVVSNEGTGTLVLVSLTSNSVVGRIDAVRASDDDDKDDHSDHE
                     RASNVPTVQTLSPTSAKAGATFTVTITGTNLTGATDVSFGGSQGKGNGNDRSSDFMVK
                     NIVVSASGTQLTASVSIDASAKPGGRVVRVETPNGESTEKGVGAVVFTVLP"
BASE COUNT          279 a          515 c          471 g          309 t
ORIGIN      
        1 aacggtgtcg ctatcgcgcc gcctcatagc cgagatgcca gccgagtttc tccgcatcga
       61 accacgagat atcgattccg aaccagcggc tcttagatgc cgctttgtaa gggcccggac
      121 gggtcgcgcc gagaatgaac gcgcacggac cgtcgccaaa cttctgaacg cgctcgtcga
      181 gccacgtccc agcatgcaac ggctgcgcga atacgaccgg cgcatctcct acaatcgcca
      241 gttgggcgcc aaagtccttg tcgacctgct taatggcagc cggaagcgcg aacgccttgc
      301 gatactcttc gacggccgcg tcaaggttgc gcaccgcgat cacaatgcgg gttactgctt
      361 tgaaatcccg cgtggtgggc tttcctgccg gaaacgcgcg ctgctcgcgc ggtgtaaagt
      421 cgtgaatgaa aaaggggaag aaactgcccc gaacagccgt accgagatcg gcggtttccc
      481 aatcgaggcg aacgccatcg ggccgctgcc gaccggcgcg gacgggctcg gaaacggtaa
      541 cgcccgccgc tttcaggcgt ttacgatccc cggcgaggtc cttgctctga accgcccagg
      601 cgcacggcgt cccgtcctct ttcaggaatt gggcccactc gtggcgatca actgctttgg
      661 gatcggcctt tggctggatg gcgatcgctt cgagatagga tccatcggcg aagctcgcga
      721 gcgccatctc ggtgacgccg ttggcgtgcg gacctccgta aatggtgggt atgccgacgg
      781 tcgcgagcgc cgcctgcatc ttcttgaggt cgcggcccgc gacggtcgca tgatcgatct
      841 tgaggtcggc cgcgcagagc agcgcgggta tcgccagaag ccacaatcgc atgcctctga
      901 ttgtacccgc gcgccctgcg ccgctatgct cgtgcgatta cggcagaacc gtgaacacta
      961 ccgctccaac ccccttctcg gttgactcgc cgttaggcgt ctcgactcgc acgactcgtc
     1021 cgcccggttt ggctgacgca tcgatcgaaa cgctcgcggt caactgcgta ccgcttgccg
     1081 acaccacgat gtttttcacc atgaaatcgg aggagcggtc gttgccgttc cctttgccct
     1141 ggctgccgcc gaagctgacg tcggtggcgc cggtcagatt cgtgcccgtg atcgtcaccg
     1201 tgaacgttgc gccggcttta gcgctggtcg gtgaaagtgt ttgcacagtc ggcacgttgg
     1261 aggcgcgctc gtgatcgctg tggtcgtcct tatcatcgtc atcgctggcc cgcacggcat
     1321 caatgcgccc gaccaccgag ttcgacgtca agctgaccag cacgagagtc ccggtgccct
     1381 cgttgcttac gaccagcagg ttgtcctttg tgtcgatggc gagcgcccgc gcccctagat
     1441 ctaccttgac ggccgtaatt gcgccagcag ccgcccccgt cgccgggttg accggcagca
     1501 ccgagataga tccgcccgct tgattggcaa agtaaacctt gccattgaag tagaccgcat
     1561 cacttaaccc gccg
//
LOCUS       DATIMH010000430         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_967238, whole genome shotgun sequence.
ACCESSION   DATIMH010000430 DATIMH010000000
VERSION     DATIMH010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967238"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1298)
                     /locus_tag="VLI90_07340"
     CDS             complement(219..1298)
                     /locus_tag="VLI90_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSV14057.1"
                     /translation="MAETHQVVTKVWIAPGCIVCDSCENDCPEVFDVQEQTCVIRPPA
                     MNATFLQTLTPSVQVAAEGCPVDVIKFETKEVEGPAPWPKEPAEGAPAAASAAHAEVK
                     PAKVAAPTAPPDPRWQALLSNSKISPSKSGPGFAVELKKDPEVVAGQEIIRAVDKSGV
                     DWRNAPPDQRLAVLAVGGAYQPPVPLAERVRNRSKESTTRRQFNLALVVAWGAIAATT
                     ATGLAMFQDFFGPKVLKEPKKVYRVGRLEDFGNANTVFEQYKPIGIWMVNLLPGEQKL
                     IALSTICTHLGCIPNWLGDKFKCPCHGSGYYITGVNFEGPTPRPLERFAISKDSDGFI
                     VVDMTRVFRSELGEWDSPDSFVSLG"
     gene            complement(1646..2695)
                     /locus_tag="VLI90_07345"
     CDS             complement(1646..2695)
                     /locus_tag="VLI90_07345"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSV14058.1"
                     /translation="MPSDVKRSTSATAPVEPLIEPPSVKLIVRLFVIPFFIVAVAVGI
                     MFLIGRMAGGEPTLDEALARLKSPGGERTADVLVGPASKQRYIDAKTVVDKMKLGMSE
                     AERVTLTRDLIDIIDNYTRSDEGDVRHFMLLALGRAWQRMPSDPPMDSPAAIEARQRA
                     MATILKYADDPQIMTRKAAILSLGFWSNREEAKQAIPKLIEKVRDEREDLDVRMAAAT
                     SLGPIASPNDAAVLDALRFATRDTDPKDIELVWDASLSLAQLNQPDSAETIEGLLDRD
                     QLAKVQVYDRDTDPKNPVFRSLSDAEVQRILINTMIGASKLQVPAVQERIRKLAESDP
                     SARVREAAQEVLHSH"
     gene            complement(2735..3553)
                     /locus_tag="VLI90_07350"
     CDS             complement(2735..3553)
                     /locus_tag="VLI90_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV14059.1"
                     /translation="MSIESPIATADTPAPAAAPVIFGADGPDVVVEGPVVTKVSIVIP
                     VYNEEHTVQKLVGLVVRAPLPTGVSREIVCVNDCSKDGTAKKLDELAKLFPEVEFRIQ
                     HKPVNEGKGAALRDGFKLAGGDVVLIQDADLEYDPKDYRKLLEPIVQGKADVVYGSRF
                     IGEPHRVLYFWHTLGNKFLTAFSNMFTNLNLTDMEVCYKVFRKPVLDAIDIKCNRFGF
                     EPEITAKIAKFKPRLRIYEVGVAYYGRSYEEGKKITWKDGIKAILTIIRFRFMD"
     gene            3708..>4409
                     /locus_tag="VLI90_07355"
     CDS             3708..>4409
                     /locus_tag="VLI90_07355"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HSV14060.1"
                     /translation="MTEATKSPPRNAAGFNYTCLAVVFLVALALILRPIWNEKKHHDF
                     GQFYMGATMARLGEWSAIYPEPVPTSNVNPGWPRGSTMKPAYAAAAAERGVDNTFRYI
                     QLPPNALLYYPLALLSVQNAYRLWHAVMICFIVITAALAGQIYDAMRGGDDDDGRRSI
                     AAGLVTLLVGCSPLMIYTARVSNTGPLLSACFGAAILGMLRPERRRPWGTALGVTIGG
                     MTKYAPGVLVPVLFSM"
BASE COUNT          836 a         1440 c         1290 g          843 t
ORIGIN      
        1 ttgtcagttg tcagttgtta gttgtcagtt gtcagttgtt agttgttgtt tcgcacaaga
       61 ccacgtctcg ccagcggacc acggacaacg gacaacagac cactgacaac taacaactga
      121 caacagacca cagaccactg accactgaca actaacaact aacaactaac aactgacaac
      181 tgacaactga caactaacaa ctgacaaccg accaaggact aaccaagtga aacaaacgaa
      241 tccggactat cccattcgcc cagttctgag cggaagacgc gggtcatatc gacgacgatg
      301 aagccgtcgg agtctttgct gatcgcgaag cgttccagcg gccgcggggt cgggccttcg
      361 aagttcacgc cggtgatgta gtaaccgctg ccgtggcagg ggcacttgaa cttgtcaccc
      421 agccagttgg ggatgcagcc caggtgcgtg cagatcgtgg acagcgcgat cagcttctgc
      481 tcgcccggca gcaggttcac catccagatg ccgatcggtt tgtactgctc gaacaccgtg
      541 ttggcgttgc cgaaatcttc cagacgaccg acgcgataca ccttcttcgg ttccttcagc
      601 accttgggcc cgaagaagtc ctggaacatc gcgagccccg ttgccgtcgt cgcggcgatc
      661 gcgccccacg ccaccaccag cgccaggttg aactgccggc gcgtcgtcga ttccttcgat
      721 cgatttcgca cgcgctccgc cagcggcacc ggaggttgat acgcgccccc caccgccagc
      781 actgccagcc gctggtccgg cggcgcgttg cgccaatcga cgccggactt atcgaccgcg
      841 cggatgatct cctgccccgc aacgacttcc gggtctttct tcaactcaac cgcgaacccc
      901 ggccccgact tcgacgggct gatcttgctg ttcgacagca atgcctgcca gcgcgggtcc
      961 ggcggcgctg tcggcgctgc caccttcgcc ggcttcactt ccgcatgcgc agcgctcgcc
     1021 gccgccggcg cgccttcggc cggttccttc ggccagggcg cggggccttc cacttccttg
     1081 gtttcaaact tgatcacgtc aaccgggcaa ccctcggcgg cgacttgaac cgacggcgtg
     1141 agcgtctgca gaaacgtcgc gttcatcgcc ggtggacgga tgacacacgt ctgctcctgg
     1201 acgtcgaaga cctccgggca gtcgttctcg cacgagtcgc aaacgatgca cccgggcgcg
     1261 atccaaactt tggtgacaac ttgatgtgtt tcagccatac gtcgatccgc ggaaacgaac
     1321 gaaggctaga gcaaggaata gtttacggac tcggggcagg ggtggcaaca actatcggcg
     1381 gttgcgccgg ggtgcaggac agggcaaacg catgtaggaa cggcgcgctc tggtcccctc
     1441 tcccggtact gctgaccgca ggtcgccggg agaggggacc agaggagggc ccattcacaa
     1501 agttcaatta caccaggggt gcatgttgca ctcatcgaaa agtgctacaa agtgcaacat
     1561 ggtgcaacat ttgggggggg cgcgaaaaaa tgtcggacat ccccgggcct tcgcaatacc
     1621 tcggacaccg gcgtcaattg agcactcaat gcgagtggag aacttcctgc gccgcttccc
     1681 gaacccgcgc tgacggatca ctctcagcaa gtttccgaat ccgctcctgc accgccggga
     1741 cctgcagctt gctcgctccg atcatcgtgt tgatcaggat ccgctgcacc tccgcatcgc
     1801 tcaggctgcg aaagacgggg ttcttcggat ccgtgtcccg atcgtacacc tgcaccttgg
     1861 ccagttgatc gcggtcgagc aatccttcga tcgtctccgc cgagtcaggc tggttcagct
     1921 gggccagcga cagcgacgca tcccacacca gctcgatatc cttcgggtcc gtgtcgcgcg
     1981 tcgcgaatcg cagcgcatcc agcaccgcgg cgtcgttcgg tgatgcgatc ggtcccagcg
     2041 acgtggcggc cgccatgcgc acgtccaggt cttcgcgctc gtcgcgcacc ttttcgatca
     2101 gcttggggat cgcctgcttc gcctcttctc ggtttgacca gaaaccgagg gacaggatcg
     2161 ccgccttgcg ggtcatgatc tgcgggtcat ccgcgtactt taaaatcgtc gccatcgcgc
     2221 gctgccgggc ttcgatcgcc gcgggggaat ccatcggtgg atcgctcggc atccgctgcc
     2281 acgcccggcc cagcgccagc agcatgaaat gccggacgtc cccctcgtcg ctgcgcgtgt
     2341 agttgtcgat gatgtcgatc aggtcgcggg tcagggtgac gcgctcggcc tcgctcatgc
     2401 cgagcttcat cttgtccacg acggttttcg cgtcgatata gcgctgcttg gacgccgggc
     2461 ccaccagtac atccgcggtg cgctcgccgc cggggctctt cagccgggcc agcgcttcat
     2521 ccagcgtcgg ttccccgccc gccatccggc cgatcaggaa catgatcccg accgcgacgg
     2581 cgacgatgaa gaacgggatg acgaacaggc ggacgatcag cttgaccgac ggcggttcga
     2641 tcaacggctc caccggcgcg gtggcgctgg tggatcgctt cacgtcagat ggcatgggga
     2701 gaggggaatc catcggtcgc cacatcacgc cgggtcaatc cataaaccga aaccggatga
     2761 tcgtcaggat cgccttgatc ccgtctttcc acgtgatctt cttcccctct tcgtagctgc
     2821 gcccgtaata cgccacgccc acttcgtaaa tccgcagccg cggtttgaac ttggcgatct
     2881 tcgcggtgat ctccggctca aagccaaatc ggttgcattt aatatcaatg gcatccagca
     2941 caggcttgcg gaagaccttg tagcaaacct ccatgtccgt caggttcagg ttcgtgaaca
     3001 tgttgctgaa cgcggtgaga aatttgttcc ccagcgtgtg ccagaaatac agcacgcggt
     3061 gcggctcgcc gatgaaacgc gacccgtaca ccacgtccgc cttgccctga acgatcggct
     3121 ccagcagctt tcggtaatcc ttcgggtcgt actcaaggtc agcatcctgg atcagcacga
     3181 catctccgcc ggccaacttg aagccgtcgc gcagcgcagc gcccttgcct tcgttcaccg
     3241 gcttgtgctg gatgcgaaat tcaacttccg gaaaaagttt cgccagctcg tcgagcttct
     3301 tcgccgtgcc gtctttcgag cagtcgttca cgcagacgat ctcgcgcgac acgcccgtcg
     3361 gcagcggcgc gcggacgacc agccccacca gcttctgcac cgtgtgctct tcgttataga
     3421 ccgggatgac gatcgagacc ttcgtgacca ccgggccttc aaccacgaca tccggcccgt
     3481 ccgcgccgaa aatgactggc gccgcggcgg gagccggggt gtcggcggtc gcgattggag
     3541 attcaatcga catcgtcgca ggtcctttca acatccgaac caaagcgggt aggatcgcgc
     3601 ggcggattgt atcggtgccg ccataggcgt caaaccggtg aaatcgccgc ttaagtgatg
     3661 acatcggccg gacgatctaa agacttaagc gcgcagaagt tcctgacatg accgaagcaa
     3721 ccaaaagccc gccccgcaac gccgcggggt tcaattacac ctgcctggcc gtcgtcttcc
     3781 tggtggcgct ggcgttgatc ctccggccga tctggaacga gaaaaaacac cacgacttcg
     3841 gccagttcta catgggcgcg acgatggccc ggctgggtga atggagcgcg atctaccccg
     3901 agccggtgcc gacatcgaac gtgaaccccg gttggccgcg gggctcgacg atgaagcccg
     3961 cctacgccgc cgccgccgcg gaacgtggcg tggacaacac ctttcgttac atccagctgc
     4021 cgcccaacgc gctgctgtat tacccgctgg cgctgctgag cgttcaaaac gcatatcgac
     4081 tctggcacgc ggtgatgatc tgcttcatcg tgatcaccgc cgcgctcgcc gggcagatct
     4141 acgatgcaat gcgcggcggc gacgatgacg acggtcgccg cagcatcgcc gccggtctgg
     4201 tgacgctgct ggtcggttgc tcgccgctga tgatttacac cgcgcgcgtg agcaataccg
     4261 ggccgctgct cagcgcgtgc ttcggcgcgg ccattttggg aatgctgcgc ccggaaagac
     4321 gccggccctg gggcacagcg ctgggcgtga cgatcggcgg gatgaccaag tacgcccccg
     4381 gcgtgctcgt gccggttcta ttttcgatg
//
LOCUS       DATIMH010000431         5260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2409422, whole genome shotgun sequence.
ACCESSION   DATIMH010000431 DATIMH010000000
VERSION     DATIMH010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409422"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1258)
                     /gene="moaA"
                     /locus_tag="VLI90_07360"
     CDS             complement(173..1258)
                     /gene="moaA"
                     /locus_tag="VLI90_07360"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019008 - molybdopterin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HSV14061.1"
                     /translation="MSAPPVFDTGPQSIAAVRVLRISVTDRCNFRCLYCMPEEGVRWL
                     PREDILSFEEIADVVRAAIAVHGIRRFKLTGGEPTVRYGFIDLVRQLRRIDGIDDLSL
                     TTNGMLLEDLAGPLKESGLDRVTVSIDSLRADRFKRITRTGDLTTVMRGLERAEDVGL
                     ANLKINCVTMRGTNDDEVAEFARLTLTRGLTVRFIEYMPLGDAALMHSTTNRIDAAEI
                     GPAGGCGAQDRGQDSFISEVDVRRRIEDELGPLVPVDRREEAGVGPANVYRLANGDPR
                     GRIGFISAMSAPFCSTCNRLRLTADGVLRSCLFEGGEVGIRDLLRRHADAHERREAIA
                     HAMAECVRLKPDVHSHRGNQQMSRIGG"
     gene            1545..3398
                     /locus_tag="VLI90_07365"
     CDS             1545..3398
                     /locus_tag="VLI90_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14062.1"
                     /translation="MRTIQRAIRSSRSLVLGLAAAVALATASTARAAGVLDQVPSDAA
                     VVWKINHLQDTSTKLAGLFQALGITDFVPTMSDPLTAIQNETGMSAGVDKAGDAAAVL
                     LNGHWDQMDEKPPIVILMPVSDYKAFVAGMTPVRTEGDVAVVHFKNDQSTDTFVANWG
                     SYAAISPMKEAVAKPASAGLKAGGLAGKQLAERDAVIYVNWPVLKPVLLPKLQQGHQQ
                     LLQEVDRAFAHKANLSRTGGGGDPSPVLKALVDQAVNVAQSFLTDSTATTWGFSIEKA
                     GISSTMLSEFSPGSYAANMAGQVKSTDQSVMNGLPSDKYLFYGGGVMSPEVGVKALDD
                     FSAPIVKELGSMGTQGDAIKALVDSYRDAIQASQRGAFGMVAPNPNTPGGLFQIVGVY
                     KGDAEKLKAAQIKQVQTQDAVMKSLGVPNSDLQKTTVTPNASTVDGVSFDEMKIAFDG
                     QAKTAQAMQAQQAMAFMYGPNGFIMRTAVIDPKTMVSIAGGNDELVSKVVAAAKADKD
                     VLSQTDVVKGVDAELPKTRAFAMYIPLDTIVSTAVGYAGKFGFPVPVQLPPNLPPIGV
                     TAGTEGSAYRVDGYVPTQLIQSLIQAGMQVYLQMRGGQGGGGGGGAGGGGL"
     gene            complement(3620..5131)
                     /gene="der"
                     /locus_tag="VLI90_07370"
     CDS             complement(3620..5131)
                     /gene="der"
                     /locus_tag="VLI90_07370"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HSV14063.1"
                     /translation="MSSMPTVAIVGRPNVGKSSLLNALTGKRISIVQDMPGVTRDRIS
                     IPLKIDDRYVELVDTGGYGFVDPDQLTEHINHQIELAMTQADLVLFVVDCQDGLTSAD
                     ETIAALLRRKDIKTVLIANKADGVKADVILGDFARLGLGTPIGVSALNDRNIDEVFSA
                     IKRNVDFANAPTEVPEPQMHVAIVGKRNAGKSTLVNSIAELYQGDPERVIVSEVPGTT
                     RDSVDVRFEKDGKTLIVIDTAGVRKRRHMMTNDIEFYSFHRAERSIRRADVVLMLIDG
                     TEPVSEPDKKLAAYIAEQYKPVIIVVNKWDLARQRAREQRKDAKDHGVDDSALMEEFG
                     EYLAAELQQIDYAPVAFVTAKDGRNIQAVLDLTQHLFKQASERLSTSKLNQAVRQILD
                     ERRPPTPTGRRARVYYATQAEVAPPTIVLFVNNPALIDEMYQRYMINRFRELLPYAEV
                     PIKLVIRGREGRSAEGELTRDADERPTKARPKTVKKAPRKMARRPPSRNARRK"
BASE COUNT          948 a         1636 c         1689 g          987 t
ORIGIN      
        1 agagcaccgc gtccgcgtgc aggcggagat ggatcgcgtg caggcgcggg cggaggcgat
       61 tgcgctgaat cgaacgtgac cgacggtggt tcttgaagaa ggggagccgg gccgtcctcg
      121 gcccggtctt atgcaaaccg gggcgcggac gccccggctc tcctggtagc attcaacccc
      181 cgattcggct catctgctgg tttcctcgat gcgagtgcac atccggcttc aaccgcacgc
      241 actccgccat tgcatgcgcg attgcctctc gccgctcgtg cgcatccgcg tgccgtcgca
      301 gcaaatcgcg aatgccgact tcgccgcctt cgaagaggca ggaccgaagc acaccgtcgg
      361 ccgtcaggcg caggcgattg cacgtcgagc agaacggcgc ggacatcgcg ctgatgaagc
      421 cgatgcgccc gcgcggatcg ccgttggcaa ggcgatagac gttcgccggc ccaacgccgg
      481 cttcttcgcg gcgatcgacc ggcaccagcg gcccgagctc atcctcaatc cgccggcgta
      541 catccacttc gctgatgaac gaatcctgcc cgcggtcctg cgcgccgcag cccccggcgg
      601 ggccgatctc ggcggcatcg attcggttgg tggtcgaatg catcaacgcc gcgtcgccca
      661 gtggcatgta ctcgatgaag cgaaccgtca atccacgagt cagcgtcaat cgcgcgaact
      721 ccgcgacctc gtcatcgttc gtgccgcgca tcgtcacgca gttgatcttg aggttggcca
      781 ggcccacatc ttcagcgcgc tcgagcccgc gcatgacggt cgtcaaatcg cccgtgcgcg
      841 tgatgcgttt gaatcgatcg gctcgaaggc tgtcgatgct gaccgtcacg cgatcgagcc
      901 cgctctcctt cagcggcccc gcgaggtctt ccagcagcat tccgttggtg gtgagcgaga
      961 gatcgtcgat cccatcgatc cgtcgcagct ggcggaccag atcgatgaag ccgtagcgga
     1021 cagtcggttc gccaccggtc agtttgaatc tgcgaatacc atgcacggcg atcgccgcgc
     1081 ggacgacgtc cgcgatttct tcgaacgaaa ggatgtcctc ccgcggcagc cagcggacac
     1141 cttcttcagg catgcagtac agacagcgga agttgcagcg gtcggtcacg ctgatgcgca
     1201 gcacgcgcac cgcggcgatt gactgcgggc ccgtgtcgaa cacgggtggg gcgctcaaag
     1261 cgctcggcga cggttgctga gacaaaaccg gtagacgcat cggtcgcatt gtagcattgg
     1321 cgcgtcgttc gtgtcgaagg agaaaagcac acggatagct atccgcgggc tttgcgctcg
     1381 gcgcggcgcc gcaacgaaaa ttccccacat cggcgccgtc ccagttataa ggacttttcc
     1441 tgattgtaaa ctacggcggt ttgacgacct tcgtatgctg gttaccattg ccgccgattc
     1501 gggaacggga ataatctcga caacacttca gagaaggagc ttcaatgcga acgattcaac
     1561 gtgcgatccg ctcgagccga tcgctggtgc tcgggctggc ggccgcggtc gcgctggcga
     1621 cggcatccac cgcgcgggcg gccggtgtgc tggaccaggt gcccagcgat gccgccgtcg
     1681 tctggaagat caaccacctg caagacacca gcaccaagct ggcggggttg ttccaggcgc
     1741 tgggcatcac agatttcgtg ccgacgatgt ccgatccgct gacggcgatc cagaacgaaa
     1801 ccggcatgtc ggccggcgtg gacaaagccg gtgacgccgc cgcggtgctg ctcaacggtc
     1861 actgggacca gatggacgag aaaccgccca tcgtgatcct gatgccggtc tcggattaca
     1921 aggccttcgt cgccggcatg acgccggtgc gcaccgaagg cgatgtcgcg gtcgttcatt
     1981 ttaagaatga tcagtccacc gacacgttcg tcgccaactg gggttcgtac gcggcgatca
     2041 gcccgatgaa ggaagccgtc gccaaacccg cgtccgcggg tcttaaagcg ggcggtcttg
     2101 ccggcaagca gctggccgag cgcgatgcgg tcatctacgt caactggccg gtgctcaagc
     2161 cggtgctgct gcccaagctt caacaggggc accagcagct gctgcaggag gtggatcgcg
     2221 cctttgctca caaggcgaac ctgagccgca cgggcggcgg gggggatccg tcgccggtgc
     2281 tgaaggcgct ggtcgatcaa gccgtcaacg tcgcccagag tttcctgacc gattcgaccg
     2341 ccacgacctg gggcttctcc atcgaaaagg ccggcatcag cagcaccatg ctgtctgagt
     2401 ttagccccgg ttcatacgcc gccaacatgg ccgggcaggt caagagcacc gatcaatctg
     2461 tgatgaacgg cttgcccagc gacaagtacc tgttctacgg cggcggcgtg atgtcgccgg
     2521 aagtcggcgt gaaggcgctg gacgatttct ccgcgccgat cgtcaaggaa ctgggttcca
     2581 tgggcacgca gggcgacgca atcaaggccc tggtcgattc ctatcgcgat gcgattcagg
     2641 cgtcccaacg cggcgcgttt ggcatggtcg cgccgaatcc gaataccccg ggcggattgt
     2701 tccagatcgt cggcgtctac aagggcgacg cggaaaagtt aaaggccgcc cagatcaagc
     2761 aggtgcagac gcaggatgcg gtgatgaagt cgctgggcgt gccgaattcg gatctgcaga
     2821 agacgaccgt cacgcccaac gccagcacgg tggacggcgt ctcgttcgac gagatgaaga
     2881 tcgccttcga cggtcaggcc aagaccgccc aggcgatgca ggcgcagcag gcgatggcgt
     2941 tcatgtatgg cccgaatggt ttcatcatgc gaaccgcggt gatcgacccc aagacgatgg
     3001 tcagcatcgc cggtgggaat gacgagctgg tgagcaaggt ggtggccgcc gccaaggcgg
     3061 acaaggacgt gctgagccag acggacgtcg tgaagggcgt ggatgcggaa ttgcccaaga
     3121 cgcgggcgtt cgcgatgtac atcccgctgg acacgatcgt ctcaacggcc gtcggttacg
     3181 ccgggaagtt cggctttccc gtgccggtgc agttgccccc gaacctcccg ccgatcggcg
     3241 tgaccgcggg caccgaaggc agcgcgtatc gcgttgacgg ctacgtgccg acgcagctga
     3301 ttcaaagcct gatccaggcc ggtatgcagg tttacctgca aatgcgcggc ggccaaggcg
     3361 gcggtggtgg cggtggcgcc ggtggtggtg ggctgtaaag gccaccccga agtaaacaag
     3421 cgcaagaagc tggccgcgac caacgtcgcg gccagtttcg ttcgcgtggg tcgctggaag
     3481 taaaaccacc ccatttagcc cggcatgaac acacgccggg tcatgttgag aagtggcgcc
     3541 ggttggaatt ctcagataat ggtgcgcgaa gatccgagca aatccggcgt gtgttcacgc
     3601 cgggagaaac aggggcgttt tacttccgcc gggcgttgcg cgaagggggc cttctcgcca
     3661 tttttctcgg cgcctttttc accgtcttcg gccttgcctt cgtcggccgc tcatctgcgt
     3721 cgcgcgtcag ctcgccttcg gcgctgcgcc cttcgcggcc gcggatgacc agcttgatcg
     3781 gcacctccgc gtacggcagc aattctctaa agcggttgat catgtagcgc tgatacatct
     3841 catcgatcaa cgccgggttg ttcacgaaca gcacgatcgt cggcggggcg acctccgcct
     3901 gcgtcgcgta atacacgcgc gctcttcgtc ccgtgggtgt gggcgggcgt cgctcgtcga
     3961 gaatctgtcg aaccgcttga ttcagttttg aagtggacag ccgttcgctc gcctgcttga
     4021 acaaatgctg cgtgagatcg agcaccgcct ggatgttccg cccgtccttc gcggtgacga
     4081 acgcgaccgg ggcgtagtcg atctgctgca gctcggccgc caggtattca ccgaattctt
     4141 ccatcaacgc cgaatcgtcc acgccgtggt ccttcgcgtc cttgcgctgc tcgcgagcgc
     4201 gctggcgcgc caggtcccat ttgttcacga cgatgatgac cggcttgtac tgctccgcga
     4261 tgtatgcggc caatttctta tcgggttcgc tcaccggttc cgtgccgtcg atcagcatca
     4321 gcacgacgtc cgcccggcgg atcgagcgct cggcgcggtg gaagctataa aactcgatat
     4381 cgttggtcat catgtgccgc cgcttgcgca cgccggcggt gtcgatgacg atcagcgttt
     4441 tgccgtcctt ttcgaagcgc acgtccacgc tgtcgcgcgt cgtgcccggc acctcgctga
     4501 cgatcacgcg ctcggggtcg ccttggtaca gctcggcgat cgagttcacc agcgtgctct
     4561 tgccggcgtt gcgcttgccg acgatggcca cgtgcatctg cggctcgggc acctcggtcg
     4621 gggcgttggc gaaatcgacg ttgcgcttga tggcgctaaa cacctcgtca atgttgcgat
     4681 cattcaacgc cgacacaccg atcggcgtgc ccaggcccag ccgggcgaag tcgccgagga
     4741 tcacgtcggc cttcacgccg tcggccttgt tggcgatcag caccgttttg atgtccttcc
     4801 gccgcagcag ggcggcgatc gtctcatcgg cgctggtcag tccgtcctgg caatcgacga
     4861 cgaacagaac gagatccgcc tgcgtcatcg ccagttcgat ctggtggttg atgtgctccg
     4921 ttagctggtc cggatcgacg aagccatatc caccggtatc gacgagttcg acgtagcggt
     4981 catcgatctt cagcgggatg gatatgcggt cgcgcgtaac gccgggcatg tcctggacga
     5041 tggaaatccg cttgccggtc aaggcgttca gcaggctgct cttgccgaca ttcggtcggc
     5101 ccacaatcgc aacggtcggc atggaagaca tgggggcact atagcggcta gcaggagcgg
     5161 cgtcagtctg cgcgcgtgcg ggagggtcgc agcgtcttgg gtcgtgacgc ttgggattca
     5221 ataaacttca tcatgggtgc cgaccgaaag cagcagaatt
//
LOCUS       DATIMH010000432         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_390660, whole genome shotgun sequence.
ACCESSION   DATIMH010000432 DATIMH010000000
VERSION     DATIMH010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390660"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..131
                     /locus_tag="VLI90_07375"
                     /pseudo
     CDS             1..131
                     /locus_tag="VLI90_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
     gene            198..1829
                     /locus_tag="VLI90_07380"
     CDS             198..1829
                     /locus_tag="VLI90_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV14064.1"
                     /translation="MRRRFETVVENLPFGVVVVEPGGRIVLTNQALTILVGHAPIPTR
                     TVAEFAQWPMLRPDNTPLPPQEHPVARALAGELVQNQEVQYRRGDGRTIWIRASGIPV
                     RDAAGAVTGAIGVAIDVDQEKRSELELRRLNETLEHRVAAETADRMKAEDSLRQSQKM
                     EAIGQLTGGVAHDFNNLLQVVMGNLESLQRRAASSGAISAADLERLTENALSGARRAA
                     LLTQRLLAFSRRQPLEPRSLDANALISGTAELLRRTLGESIALESVLAGGLWRISADP
                     NQLENALLNLAVNARDAMPNGGRLTIEAANVFLDEAYAAREQVSPGQYVMIAVSDTGT
                     GMSAEVIGRAFEPFFTTKEVGQGTGLGLSQIYGFVKQSDGHVKIYSEPGDGTTIKLYL
                     PRLHAAEAERETLIQPQPVGGALDEVILAVEDDPDVRVNTVGMLRELGYRVLEATDGH
                     SALELLQRHPEVRLLFTDVGLPGGMNGRQLGDDARARCPDIRVLFTTGYARNAIVHQG
                     RLDPGVALLVKPFTYAGLAAKLREMLDQTAIADAG"
     gene            1941..2264
                     /locus_tag="VLI90_07385"
     CDS             1941..2264
                     /locus_tag="VLI90_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14065.1"
                     /translation="MVKAVGIDFGTTNSVVALLHDDGSVTTQRHASLDVFRSLLCFWT
                     EPRPGRSVLHHAAGPEAITAYLEDPLDSRLIMSMKTFLAQRSFTETRIFGHRFRIPRY
                     HAGER"
     gene            2271..2492
                     /locus_tag="VLI90_07390"
     CDS             2271..2492
                     /locus_tag="VLI90_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14066.1"
                     /translation="MTENEKERLIREYAAGEVTWSTLRERGFDNFVDVLADLGKLGLR
                     QPVAPMDGPNREARERGRAIIREALKATR"
     gene            2489..3022
                     /locus_tag="VLI90_07395"
     CDS             2489..3022
                     /locus_tag="VLI90_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14067.1"
                     /translation="MTRRLGLIVTDASPLITLAAAEALECLTMPGLPVVIPDMVYFEV
                     TQDFAKLGVGEIVDWARKHHGQVEIVPTTVFAEFQALRATNPRTRSKGRGEQAALEVL
                     NAAIADDRDIEAVLFFEDSDVRKRRFVRGLPERVTALSTGDLLHELEAAGRIQSSDRI
                     LDLAAAKDCQSALNSFH"
BASE COUNT          578 a          992 c          983 g          579 t
ORIGIN      
        1 tccccatcgt cctgctctcc gcccgcgccg gcgaggaagc gcgcgtcgaa ggtcttgaat
       61 ccggcgccga cgactacctg accaagccgt tcagcgcccg cgaactggtc gcccgtgtcg
      121 atacgaatct tggtatggca cagcttcggc gcgaggccac tttgcgcgag cgcgcactgc
      181 gcgcagaggc cgaaaccatg cgccgacggt ttgaaaccgt ggtggagaac ctgccgttcg
      241 gcgtcgtcgt ggtcgaaccc ggcggacgca tcgtgctaac caatcaggcc ctgacaattt
      301 tggttgggca cgccccgata ccgacacgca ccgtcgccga attcgcacaa tggcccatgc
      361 tgcggccgga caacacgccg ctgcccccgc aggaacaccc ggtcgcccgc gcacttgccg
      421 gggaattggt gcagaatcaa gaggttcaat accgtcgtgg cgacggccga accatctgga
      481 tccgcgcctc cggaattccc gtgcgcgatg ccgccggtgc ggtaaccgga gccatcggcg
      541 tcgccatcga tgtcgatcag gaaaagcgct ccgaactcga actgcgccgc ctgaacgaaa
      601 cgctggaaca ccgcgtcgcc gccgaaaccg ccgatcggat gaaggccgaa gactcgcttc
      661 gccaatcgca gaagatggag gcgatcggtc agcttaccgg cggagttgcc catgatttca
      721 acaatctgct gcaggttgtt atgggcaatc ttgaatcgct gcagcgtcgc gccgcgagtt
      781 ccggcgcgat ctccgccgct gatctggagc ggctcaccga aaacgctctg agcggcgcgc
      841 ggcgggccgc actgctgacg cagcggctgc tggcgttctc gcgtcgccag ccgctggaac
      901 cccgaagcct cgacgccaac gcactgatct ccggcaccgc agagctgctc cgtcgcacgc
      961 tgggggagag catcgcgctt gaaagcgtgc tggcgggtgg tctatggcgc atctccgcgg
     1021 atccgaacca gcttgagaac gcgctgctca acctggcggt gaatgcgcgc gacgccatgc
     1081 ccaacggcgg tcgcctcacc atcgaggcgg ccaacgtctt cctcgacgag gcttacgccg
     1141 ctcgcgagca ggtcagcccc ggccagtatg tgatgatcgc ggtcagcgac accggtactg
     1201 gcatgagcgc cgaggtcatc ggccgcgcct tcgagccgtt cttcaccacc aaggaggtcg
     1261 ggcaggggac cggactggga ttgtcgcaga tctatggctt cgtcaagcag tccgacggtc
     1321 acgtgaagat ctacagtgaa ccgggcgatg gaacgacgat caagctctat ctgccgcggc
     1381 tgcacgcggc cgaagccgag cgcgagaccc tgatccagcc gcaacccgtt ggcggcgcgc
     1441 tggatgaggt gattctggcg gtcgaggacg atcccgacgt tcgcgtcaac acggtcggca
     1501 tgctgcgcga attgggatac cgcgtgttgg aggccacaga cggccattcg gcactggagt
     1561 tgttgcagcg tcatccggag gtgcgactac tgttcaccga tgtcggtctg cctggcggca
     1621 tgaacgggcg ccagctaggc gacgacgcac gcgcccggtg tcctgatatt cgcgtgctgt
     1681 tcaccaccgg ctacgcccga aacgccattg ttcatcaggg ccggctggac cccggcgtgg
     1741 cgctgctggt caagccgttc acctatgccg ggctggcggc aaaactgcgc gagatgcttg
     1801 accagaccgc tatcgcagat gccgggtaac cgcctcgacc gcgtcgctgc aatttccata
     1861 atatcccttc tgcggcaatc ggctgtaaac ccccgatgga aatgtcaccc gtgtccccga
     1921 tagctgattg aagcctccgg atggtcaagg cggtcggcat cgacttcggc accaccaaca
     1981 gcgtcgtggc cctgctgcac gacgatgggt cggtcaccac gcagcgccac gcaagcctcg
     2041 acgtattccg ttcgctgctg tgtttctgga ccgagccgcg ccccggccgc tccgtcctcc
     2101 accacgccgc aggaccggag gcaatcaccg cctacctgga agacccgctg gacagccggc
     2161 tgatcatgtc gatgaagacc ttcctcgctc agagaagctt caccgaaacc cgcatctttg
     2221 gccaccggtt ccgcatcccg cgctatcatg ccggtgagag gtagttagcg atgactgaga
     2281 atgaaaagga aaggcttatc cgcgaatatg ctgctggcga ggtcacatgg tctacgctgc
     2341 gcgaacgcgg ctttgacaat ttcgtggacg tgctggctga cctcggtaag ctcggattgc
     2401 gccagccggt cgcgccgatg gacggaccca accgcgaagc gcgcgagcgt ggccgggcga
     2461 tcatccgcga ggcgcttaag gcgacgcgat gacgcgtcgt ctcggcctca tcgttacaga
     2521 cgcgtcgccc ctgattacgc tggccgccgc cgaggcgctt gagtgcctga ccatgccggg
     2581 tctgcccgtt gtcattcccg atatggtgta tttcgaggtg acacaggact ttgcgaaact
     2641 tggcgtcggc gagatcgttg attgggcgcg caagcatcac ggacaggtgg agatcgtgcc
     2701 gaccacggtt ttcgccgagt ttcaggcttt gcgcgcgacg aacccgcgca cgcgctccaa
     2761 gggacgcggc gagcaggcgg cgcttgaggt attgaacgcc gcgattgcag atgaccgcga
     2821 cattgaagcc gtgctgttct ttgaggacag cgacgtgagg aaacgccgct ttgtgcgcgg
     2881 cctacccgag cgcgtcacgg cgctttcgac gggcgatctg ttgcacgagt tggaagcggc
     2941 gggccgtatc cagtcgtccg accgtattct tgatcttgcc gctgccaaag actgtcaatc
     3001 agcgttgaat agttttcact gaacagcgta caaaagtttc cactttttca gcctgatttt
     3061 gagtgttttt tacgctgttt gaagcggaag gagtcgttgc ctgtttccag gatatcgcag
     3121 tggtgagtga cg
//
LOCUS       DATIMH010000433         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_102282, whole genome shotgun sequence.
ACCESSION   DATIMH010000433 DATIMH010000000
VERSION     DATIMH010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102282"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VLI90_07400"
     CDS             complement(<1..1153)
                     /locus_tag="VLI90_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14068.1"
                     /translation="MLAPFVIAMALTVVLGAGPEETRTFELRAAPPPATAGMTHQLLT
                     DPRDMRRGDAAVPLMRAALLFGNVRQEEVGKVYDARDGDPKRFDAMARPIVETMGNYV
                     TLVADAACREHADWDAGSERGINALLPELNGMRELANMLDVIATYQIHQGQLEEALNS
                     IRLQYELGRKVGQSRVLVSNLVALGIISLANDRLADVMNHADAPNLYWSLARLPRPML
                     RIDRAFEGERRMLIDTVPILAEARSRELSGDHWRDVYKQLSVLDDQSKSGKSTKQSNR
                     FDLDEQLRPGGVGQAILATAQERYAQSHNIPAGEAAELDAVKVVAAYYYEQYQNVIDG
                     MTRPINLPYPQLIPALDAWGKDAQRLMHEQPGNPFLHYVPSVNRAVARFA"
     gene            1377..>1717
                     /locus_tag="VLI90_07405"
     CDS             1377..>1717
                     /locus_tag="VLI90_07405"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HSV14069.1"
                     /translation="MAESKRFTLTDLRAARDNGKKVAMLTCYDYSTARLMHDAGVPAI
                     LVGDSAGNVILGYDSTLPVPLSFMIQITAAVRRGAPDALLVADMPFGSYHASVAQGVR
                     NVVRMVKLTGC"
BASE COUNT          292 a          599 c          498 g          328 t
ORIGIN      
        1 gcgcgaagcg cgcgacggca cgattcacgg acgggacata atgtaagaac ggattgcccg
       61 gctgctcatg catcagccgc tgcgcgtcct ttccccacgc atccagcgcc gggatcaact
      121 gcggatacgg caggttaatc ggacgcgtca tcccatcgat cacgttctga tattgctcgt
      181 agtaatacgc cgccaccacc ttcaccgcgt ccagctccgc cgcctcaccg gcggggatgt
      241 tgtgcgactg cgcgtagcgt tcctgcgcgg tggcgaggat cgcctgcccc accccaccgg
      301 gccggagttg ctcgtccaga tcgaatcggt tggattgctt tgtagacttg cccgacttgc
      361 tttgatcgtc caacaccgac agctgcttgt acacatcgcg ccaatggtcg ccgctcagct
      421 cgcgcgatcg cgcctcggcc aagatcggaa cggtgtcgat gagcatccgg cgttcgcctt
      481 cgaacgcgcg atcgatgcgc agcatcggcc gcgggaggcg cgcgagcgac caatacaagt
      541 tcggcgcgtc ggcgtgattc atcacatcgg ccaatcggtc gttcgccagc gagatgatcc
      601 ccagcgcgac gaggttgctg accaggacac gactctgccc gacctttcgc cccagctcgt
      661 attgcagccg gatggaattc aaggcctcct cgagctggcc ttgatgaatc tgatacgtcg
      721 cgatgacgtc gagcatgttg gccaattcgc gcatgccgtt cagctccggc agcagcgcgt
      781 tgatgccgcg ctcgctgccg gcatcccaat ccgcgtgctc gcggcaagcg gcgtcggcaa
      841 ccagagtgac gtaattgccc atcgtctcga cgatcggccg agccatcgcg tcgaaccgct
      901 tgggatcacc atcgcgcgca tcgtatactt tgccgacctc ctcctgccgc acgttgccga
      961 acagcaatgc cgctcgcatc agcggcaccg cggcatcccc ccgccgcatg tcgcgcggat
     1021 cggtcagcaa ctgatgcgtc atccccgccg tcgccggcgg cggcgccgcg cgcagttcga
     1081 atgtccgcgt ctcctccggc cccgcgccta acaccacggt cagcgccatc gcgatgacaa
     1141 acggagcaag cacccgtgat agcccaggcc ggaaggccgg ggatcgatct gctgattgcg
     1201 ctacttcccg tccgatccac ggccttgcgg cttgggctat taccgcaaca aatctgatcc
     1261 ctcgtcgcat ctcgccgcct ttcatctgtt cacctgcatg acgttgccga tgcatcaact
     1321 tacgtgcagc acaccatttt cgctcgcttc gctgcccgcg gctacaatgg ctgcatatgg
     1381 ctgaatcgaa gcgcttcacg ctcaccgacc tccgcgccgc ccgcgataac ggcaagaagg
     1441 tcgcgatgct cacctgttac gactactcca ccgctcggct gatgcacgat gcgggcgtgc
     1501 cggcgatcct tgtcggcgac tccgccggca acgtgatcct cgggtacgac tccacgctgc
     1561 cggtgccgtt gtcgttcatg atccagatca ccgccgccgt ccgccgcggg gcgcccgatg
     1621 cgctgctggt ggcggatatg cccttcgggt cataccacgc gtcggtcgcc cagggcgtgc
     1681 gaaatgtcgt gcgcatggtg aaacttacgg gctgcga
//
LOCUS       DATIMH010000434         3799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1546580, whole genome shotgun sequence.
ACCESSION   DATIMH010000434 DATIMH010000000
VERSION     DATIMH010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546580"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VLI90_07410"
     CDS             complement(<1..702)
                     /locus_tag="VLI90_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14070.1"
                     /translation="MDGRHGWFGSGESRAIIEGDFEHQRLSKPRITLTPTLSRRTGRG
                     GKSHIRSPTSLALENSTANATIALQFKWVGGNLRIAVRLHFGVRFTWKQIMKLTRVGN
                     AVDRLGIALGKKRKLRMLLDPDMPSLTQRPTAEGLECRLLFAIAVPTALTTFDGITDG
                     APQVGVTVSGDTIYGTTPIGGQNDDGAVYSMPITGGTPDLISGFNGTNGHQPMGDLLL
                     VNNTLYGTTSAGGANN"
     gene            780..1343
                     /locus_tag="VLI90_07415"
     CDS             780..1343
                     /locus_tag="VLI90_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HSV14071.1"
                     /translation="MQTIITGKAYVLGDNIDTDQIIPAQYLSFNPSLPEERKFFGMYA
                     MSSVPEGQRGLPAGNINFVRENEFKSDYQIVVGGKNFGSGSSREHAPLALAEAGVKCV
                     IAEFYARIFFRNSVNGGYLPPLESVERLVEKVRTGDELEVHLLDNYVINKTRGGERYA
                     LRPIGDVLPIIEAGDVFKYAKKAFNLS"
     gene            complement(1804..2724)
                     /locus_tag="VLI90_07420"
     CDS             complement(1804..2724)
                     /locus_tag="VLI90_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14072.1"
                     /translation="MKLLKTLLLIVAVLLLSAILLSAGGWFMLRGTPEWYQPSTATAE
                     QRKKAADRAENMLISIQNWAGGARAARLRAAAAAAAVGAGDAPTTTQAATALSHEPSQ
                     PFQIQFSDEELNAFFNKWADLNGRRESLERFVDDPRLVLRNNQLILAGTVKDLGTVVS
                     MQFEPRIDEHGSLQMNLVRVLGGILPLPDALWSSKRERMQQYLQAKLPAYQQEAAMMP
                     DGTANSAAASAAMNKLLQSVLHNDPADPVLFVPYDLHKLNRSVPVKITAVAIQNNTLT
                     ITAEQMTAVERGALLDRLRAPYDVATAAAR"
     gene            2920..3318
                     /locus_tag="VLI90_07425"
     CDS             2920..3318
                     /locus_tag="VLI90_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14073.1"
                     /translation="MQATLLKSPPRPTRLNGPGLSPRALPTPIIDAIERGYASHVASS
                     LNGQILRYSERQSLLRAADRLHINRFRANLIIAMVQHERGEAPAPKARSSDVPRSRGA
                     SLSTASMLAAVMLVELLLITACWWQLARAN"
BASE COUNT          719 a         1251 c         1130 g          699 t
ORIGIN      
        1 attgttagcg ccgcccgcgg aagtcgtgcc atacagcgtg ttgttcacca gcagcagatc
       61 gcccatcggc tgatgaccat tcgtcccatt gaacccggaa atgagatcag gtgtgccccc
      121 ggtgatcggc atggaataaa ccgcgccgtc atcgttttgc ccgccgatcg gcgtcgtgcc
      181 atagatcgta tcaccggaga cagtgacacc cacctgcggc gcaccatcgg tgatgccatc
      241 aaaggtagtc agcgccgtgg gaaccgcgat ggcaaacagc agacggcatt ccagtccttc
      301 agccgttgga cgctgtgtga gtgacggcat gtcaggatcg agcaacatcc gcaacttgcg
      361 ttttttcccc agcgcaatcc ccagacggtc cacggcattc cccacacgtg tgagtttcat
      421 gatttgcttc caggtaaatc tgacaccgaa atgaaggcgt accgcaattc gcaggttgcc
      481 gcccacccac ttgaactgga gtgcgatagt agcgttagcg gtagagtttt ccaatgccaa
      541 actggtcggc gatcggatgt ggctcttgcc ccctcgcccg gtacgccggg agagggtcgg
      601 ggtgagggtg attcggggct tcgatagccg ctggtgttca aaatcaccct caatgatcgc
      661 ccgggattcg cccgagccaa accagccgtg acgaccatcc acacccgact tgcctgcgct
      721 ctgtacaccg cctgctcaac gcctatcatt ccctcgttca caaccgagga cgttttctca
      781 tgcaaaccat catcaccggc aaagcctacg tgctgggcga caacatcgac accgaccaga
      841 tcatcccggc tcagtacctc tcgtttaacc ccagccttcc cgaagagcgc aagttcttcg
      901 gcatgtacgc gatgagcagc gtccccgaag gccagcgcgg cctccccgcc gggaacatca
      961 acttcgttcg cgagaacgaa ttcaaaagcg actaccagat cgtcgttggc ggaaagaatt
     1021 tcggctcggg ttcatcgcgc gaacacgccc cgctcgcgct ggccgaggcg ggcgtgaagt
     1081 gcgtgatcgc ggagttctac gcccgcatct ttttccgcaa cagcgtgaac ggcggctacc
     1141 tgccgccgct ggaatcggtc gaacgcctgg tcgaaaaagt ccgcaccggc gacgaactgg
     1201 aagtgcactt gctggacaac tacgtcatca acaaaacgcg cggcggcgag cgctacgcgc
     1261 tgcgcccgat cggtgacgtg ctgccgatca tcgaagcggg ggatgtgttt aagtacgcga
     1321 agaaggcttt caatttatcc tgagagtgtg agtttatcct gagcgaagca cgtactcgtg
     1381 aaccagtcga aggatctggc cggcgcgtcg gtgaaccgct catggacctc aagccccagg
     1441 accacgagtg ctcgtgcgcg gtgaaacctt cattcacttc tcggccatag tcttcgactg
     1501 cgcggctgcg ccgcttcgct caggataaac tcacacacgc tgagcgccgc acgtactcgt
     1561 gaaccagtcg aagcatctag ccgaagcgtc ggtgaacgat tactctcgcg gcagcgcgtg
     1621 tgagtcgctt gcggcttcgc agacgaagag cgctggatct gccaggccgc aagcgactgc
     1681 tgtatcgtct gcgcgaatgg cgcttcaccg acttctcggc tagatccttc gactccggcg
     1741 gcgtaccgcc tgacttcgac gagctcagtc gagtcgcgct caggatgaca cgcgcacgcg
     1801 cgctcaccgc gccgccgcgg tcgcgacgtc gtacggcgcc cgcagccgat ccagcaaagc
     1861 cccccgctcc accgccgtca tctgctcggc ggtgatcgtc agcgtgttgt tctgaatcgc
     1921 caccgcggtg atcttcaccg gcacgctgcg gttgagctta tgcaggtcgt acggcacgaa
     1981 cagcaccggg tcggccgggt cgttgtgcag gaccgactgc agcagcttgt tcatcgccgc
     2041 cgacgccgcg gcactgttgg cggtgccgtc gggcatcatc gccgcttcct gctgatacgc
     2101 cggcagcttc gcctgcaggt actgctgcat ccgctcacgt ttgctcgacc acagcgcatc
     2161 cggcaacggc aggatgccac ccagcacgcg caccaggttc atttgcaggc tgccgtgctc
     2221 gtcgatccgc ggctcgaact gcatgctgac gacggtgcct aagtctttga ccgtgccggc
     2281 caggatcagc tgattgttcc gcagcaccag ccgcgggtcg tccacgaatc gctccagcga
     2341 ttcccgtcgc ccgttcaggt ccgcccattt gttgaagaac gcgttcagct cttcatcgct
     2401 gaactgaatc tgaaagggct gcgatggctc gtgcgacaac gcggtcgccg cctgcgtggt
     2461 cgtcggcgca tcgcccgcgc ccaccgccgc ggcggccgcg gcggctcgca agcgcgcggc
     2521 ccgcgcgcca ccggcccagt tctggatgct gatcaacatg ttctccgcgc ggtcggccgc
     2581 cttcttgcgc tgctcggcgg ttgcggtgct gggttgatac cactcgggcg ttccgcgcag
     2641 catgaaccac ccaccagccg acagcaggat ggcgctgagc aacagcacgg cgacgataag
     2701 caagagagtt ttcagcaact tcatggcaag ccagactcgg gtgcgttcgc gtgcgggcgc
     2761 gggcgcgcgg aaagcgcgat cggccggaga atgtaccgta atcgtcggca aattcgatac
     2821 cgctccccag cttcatcaac aggatgcgat ccgaggctgt cgcgagcgac catttcccgc
     2881 tcgcgcaggg ccgatgtgcg attaagctgc acgccgaaga tgcaggcgac gttattgaaa
     2941 tccccacccc ggccgacccg cttaaacggg ccgggcctgt cgccgcgagc gctccccacg
     3001 ccgatcatcg acgccatcga gcggggctac gccagccacg tcgccagctc tttgaatggg
     3061 caaatcctgc gctattccga gcggcaatcg ctcctgcggg ccgccgaccg gctgcacatc
     3121 aaccggttcc gcgccaacct gatcatcgcg atggtccaac atgaacgcgg cgaagcgccg
     3181 gcgccaaaag cgcggtcttc cgacgtacca cgttccagag gtgcaagctt atcaaccgcg
     3241 tcgatgctcg ccgcggtcat gctcgtcgaa ttgctgctga tcaccgcgtg ttggtggcag
     3301 ttggcgcgag cgaactaaga tcgaccgagc ccttgatagc cggggccgac gaacggacac
     3361 ttttgcagaa gtgataagag accaacccac gccgggttcg gaggtacgcc gacgacccgg
     3421 atgcttcctt gcgcgccgca gaagaatctc gcttcgtcgg aatacctccg aacccggcgt
     3481 gatcggttcc caacggccgt caagccggcg gcgtaagccc cgccgcgggc gggcgcgcgc
     3541 aagatatcgt gccgcgggcc agcggcgtgc ggctgaacgt cctggcgcgg ctcgctcgct
     3601 cacggagggt gtaactccca attgtggcga gtgtccctcc ggtcccctct cccgcgtacg
     3661 cgtcgcgtac gcgcgggaga gggttagggt gagggtgatt tggaacgtcg agcggttatc
     3721 gccagaccaa atcaccctca ccctaaccct ctcccatgag tacatgggcg aggggaccag
     3781 aaaaggcctt cgccacact
//
LOCUS       DATIMH010000435         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4143664, whole genome shotgun sequence.
ACCESSION   DATIMH010000435 DATIMH010000000
VERSION     DATIMH010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4143664"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1120
                     /locus_tag="VLI90_07430"
     CDS             59..1120
                     /locus_tag="VLI90_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSV14074.1"
                     /translation="MKLFITGATGFLGQYVVAEALRRGHSVRAVARRGADLSDIDWAT
                     DANVELARVDLRSREGLVDAVRGCNAVLHLAAAKSGDVYAQLGGTVVGTENLLWAMNE
                     AGVTHLVATSTFSVYQYLQRWSHGTIDEDSPLDLAMIDRDDYAKTKLIQEQLILDYAK
                     SHNWRWTILRPAMLYGKDNLFNARVGVKAGSRWWIRTGAWARIPLNYVENCAEAIVLA
                     AEVPAANEQILNVVDDDPPTQRRYAKLLQQRTTPRPRIVPVSWTAMRSIARLAWLSNK
                     LLLGGRAKIPGLLSPAKVHARIKPFRYSNARIKQALGWRPRYSLVEAIERSLSDETPA
                     LKSTPAITPANVKLEGMTA"
     gene            1117..2403
                     /locus_tag="VLI90_07435"
     CDS             1117..2403
                     /locus_tag="VLI90_07435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV14075.1"
                     /translation="MSTFRVAYMTGEYPRATDTFIQREVATLRRLGHTIETFSIRKPA
                     SREVVSPEVQAERERTFYVLPASLPRIFAAHAGLLFKSPGRYVAGLRLAMKIRPPGLK
                     AFARQLAYFAEAGVVAQRVRQLNLQHLHNHFSNSSCSVAVMAATMGRFTFSFTMHGPA
                     EFFEPHVWRIDEKIRRALFVNCISHFARSQAMVFAPVDTWDKLHIVHCGVDPAEFEVS
                     DARSKSGSQLLFVGRLAAVKGLPILLQAIASLREAHPHLTLSLAGDGPDRQMLEKLAV
                     DLDIADRVKFLGYQSQSQVRALLQQTDIFVMTSFAEGVPVVLMEAMAAGVPVIATRIA
                     GVPELVQDGKTGVLIPPGDVAATAVAIDELLSDGDLRSRLTEAGRAKVEREFNINSES
                     MWLAQILTAALQGRRELIRRDSSVANVEGETLTATS"
BASE COUNT          474 a          834 c          857 g          461 t
ORIGIN      
        1 gttcgaaatc accctcaccc ctgccctctc ccggagtacc gggcgagggg gaattaagat
       61 gaagctgttc ataaccggcg ccacgggatt tctcggtcag tacgtcgtcg ccgaagcgct
      121 gcgacgcgga cacagcgtgc gcgccgtcgc gcgtcgcggc gctgatctgt ccgacatcga
      181 ctgggcgacc gacgccaacg tcgaactcgc ccgcgtcgat cttcgctcgc gcgaaggtct
      241 ggttgacgcc gtccgcgggt gcaacgcggt tctgcacctc gccgccgcca agagcggcga
      301 tgtttacgcc cagctcggcg gcaccgtggt cggcaccgaa aacctgctgt gggcgatgaa
      361 cgaagccggc gtcacgcacc tggtcgcgac cagcacgttt tccgtttatc aatacctgca
      421 gcgctggtcc cacggcacca tcgacgaaga ttcaccgctc gatctggcga tgatcgaccg
      481 cgatgactac gcgaagacga agctgatcca ggagcagctg atcctcgact acgccaagtc
      541 gcacaactgg agatggacga tcctccgccc ggcgatgctg tacggcaaag acaacctgtt
      601 caacgcccgc gtcggggtga aagccggcag ccggtggtgg attcgcaccg gcgcgtgggc
      661 gcggattccg ctgaattacg tggagaactg cgcggaggcg atcgtgctcg ccgccgaagt
      721 tccggcggcc aacgagcaga tcctcaacgt cgtcgatgac gatccaccga cgcagcgacg
      781 atacgcgaag ctgctgcagc agcgcacgac gccgcggccg cggatcgtgc cggtgtcgtg
      841 gacggcgatg cgcagcatcg cgcgcctggc gtggctgagc aacaagctgc tgctcggcgg
      901 gcgcgcgaag atcccggggc tcttaagccc cgcaaaggtg cacgcgcgga ttaaaccctt
      961 ccgatacagc aacgcgcgga ttaagcaggc gctcggctgg cggccgcggt attcgctggt
     1021 cgaggcgatc gaacgcagct tgtcggatga aacgccggcg ctgaaatcga cgcccgcgat
     1081 cactccggcg aacgtgaaac tcgaagggat gacggcgtga gcacctttcg cgtcgcgtac
     1141 atgaccggcg aatacccccg cgccaccgat acattcatcc agcgcgaagt ggcgacgctg
     1201 cggcggctcg gtcacacgat cgaaacgttc tccatccgca aacccgcgtc gcgcgaggtg
     1261 gtgagcccgg aagtgcaggc ggagcgcgag cgcacgttct acgtgctccc cgcgtcgttg
     1321 ccgaggatat tcgcggcgca cgcggggctg ctgtttaaat cacccggacg ctatgtcgcc
     1381 gggcttcggc tggcgatgaa gatccgcccg ccggggctga aggcgttcgc ccggcagctg
     1441 gcgtacttcg ccgaggccgg cgtcgtcgcc cagcgcgtgc ggcagctgaa ccttcagcac
     1501 ctgcacaacc atttctccaa ttccagctgc tcggtcgccg tcatggcggc gacgatgggc
     1561 cggttcacct tcagcttcac gatgcacggt ccggcggaat tcttcgagcc gcacgtctgg
     1621 cggatcgacg agaaaatccg ccgggcgctg ttcgtcaact gcatcagcca tttcgcgcga
     1681 agccaggcaa tggtgttcgc gccggtcgac acatgggaca agctgcacat cgtccactgc
     1741 ggcgtcgatc cggcggagtt cgaagtatcc gatgctcgat caaagagcgg gtcgcagttg
     1801 ctgttcgtcg gacgactggc cgcggtgaag ggattgccga tcctgctgca ggcaatcgca
     1861 tcgctgcgcg aggcgcatcc gcatttgacg ctgtctctcg ccggtgatgg gcccgaccgg
     1921 cagatgctgg agaaattggc cgtcgatctg gacatcgccg atcgcgtgaa gtttctgggt
     1981 tatcaatcac aatcgcaggt tcgcgcgctg ctgcaacaga cggacatctt cgtgatgacc
     2041 agcttcgccg agggcgtgcc ggtggtgctg atggaagcga tggccgcggg ggtgccggtg
     2101 atcgcgacgc ggatcgccgg cgtgccggag ttggtgcaag acggcaagac cggcgtcctg
     2161 attccccccg gcgacgtcgc cgccaccgcg gtggcgattg atgaattact tagcgacggc
     2221 gatctccggt ccaggctgac cgaagccggc cgggcaaagg tcgagcggga gttcaatatc
     2281 aattccgaat cgatgtggct ggcgcagatc ctgacggcgg cgctgcaggg tcggcgcgag
     2341 ttgatccggc gggattcatc tgtggcaaat gtcgaaggcg aaactctaac agccacttcg
     2401 tgacgatgcg cgcgcgtgcg cgcaaagaag ccccgcatgt ccatgcgggg cttcggtgtg
     2461 atcactgcac gatttcgagg atcttaaact cgttcgttcc gcgcggagct ttcactttca
     2521 cggtgtcccc gacgcgcgcc ttcatgagcg cttcgcccat cgggctggcg accgagacgt
     2581 tcatcacttc gctgcccggt tcgggcgccg atgcttcacc aaccag
//
LOCUS       DATIMH010000436         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2989959, whole genome shotgun sequence.
ACCESSION   DATIMH010000436 DATIMH010000000
VERSION     DATIMH010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2989959"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..877
                     /locus_tag="VLI90_07440"
     CDS             143..877
                     /locus_tag="VLI90_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HSV14076.1"
                     /translation="MIAGGLVAHPVQNARNVLRFFRETCATLPDEMMMAAGLVTAPDG
                     SGAKLAAMLAAHCGPLAQGEAALKPIKAFGPPVMDAMGPLTYCQQNGLLDPSLPKGAL
                     NYWKSHFLSDLSDGAIDAMIDAFAKCPSPMSQIVIEHFHGAASRVPVADTACAMRITG
                     FNVVLISQWADPADNERCIGWCRDTYKALGPYLGKTRYVNYLDADEDGDPAAVAYGAN
                     YARLRELKTKYDPDNVFHINVNIKPR"
     gene            910..>1779
                     /locus_tag="VLI90_07445"
     CDS             910..>1779
                     /locus_tag="VLI90_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HSV14077.1"
                     /translation="MLLTLAAVLCAHGVSAQTLTPTLQSAPSIFDPVGPPDDLLGKYA
                     ALGADDDQTTVVAKSGEKRACDGCPRRRIGTSFLQVTYVNGIYELANLIRGQDTAHIT
                     PKTWWTNMKRGWEWDLDEFVVNQFGHPYQGNNYYTTGRANGLSFWESAALTAFGSGTW
                     EYFGETNQASLNDFINTTMGGIALGEMLHRTAWLIIDPTATGGSRKKKEIFATAIDPM
                     NGINRFISGDAHRVSEKPAEFTPSSLAATTAAGVLWRGSNTADVESAAYPSVQVDLLY
                     GDPTTGRSRTPYDA"
BASE COUNT          350 a          571 c          556 g          302 t
ORIGIN      
        1 agcggaaatt gtgctcgccg acggatcgat cgtccgcgca gcggctgatg aaaacccgga
       61 tctcttctgg gccatccgcg gcggcggcgg aaatttcggc atcgcggcgt cgctcgaata
      121 cgcgctccat ccggttggac cggtgatcgc cggcggcctc gtcgcgcatc cggtacagaa
      181 cgcgcgcaac gtccttcgct tcttccgcga gacatgcgcg acgctgcctg acgagatgat
      241 gatggcggcc ggcctcgtga ccgcaccaga tggctctggt gccaagctcg ccgcgatgct
      301 ggcggcacac tgcggtccac tggcgcaggg tgaagcggcg ctgaaaccga tcaaagcctt
      361 tggcccgccg gtcatggacg cgatgggacc gctcacctac tgccagcaga acggtctgct
      421 cgatccatcg ctgcccaagg gcgccctcaa ttactggaag tcgcacttcc tcagcgatct
      481 cagcgacggc gccatcgacg cgatgattga tgcctttgcg aaatgcccgt cgcccatgag
      541 ccagatcgtc atcgaacact ttcacggcgc ggccagccgg gtaccggtgg cggacacggc
      601 gtgcgcgatg cgcatcaccg ggttcaatgt cgtgctcatc tcccagtggg ccgaccctgc
      661 agacaacgag cgctgcatcg gctggtgccg cgacacctac aaagcgctcg gcccatacct
      721 tggaaaaact cgttatgtga attacctcga cgctgacgag gacggcgatc cggcagccgt
      781 cgcctacggc gccaactacg cgcgcctgcg cgagctgaaa accaagtacg accccgacaa
      841 cgtgtttcac atcaacgtga acatcaagcc gcgttagaaa gagccgtata agggatgttg
      901 aagagactct tgctcctcac gttggcggca gtgctgtgcg cgcatggcgt ctcggcacag
      961 acgctgacgc caacgcttca gtctgcccca tcgattttcg atccggtcgg cccaccggac
     1021 gacttgctgg ggaagtatgc cgcgctcggc gccgacgacg accagacgac cgtcgtcgcc
     1081 aaatctggcg agaagcgagc ctgcgacggg tgcccccgcc ggcggattgg cacgtccttt
     1141 ctgcaggtca cctacgtcaa cggaatctac gagctggcca acctgattcg gggtcaggac
     1201 accgcgcaca tcacgccaaa gacgtggtgg acgaacatga agcggggttg ggagtgggac
     1261 ctcgacgagt tcgtggtcaa tcagttcgga catccctacc aggggaacaa ctactacaca
     1321 accggacggg ccaacgggct gagtttctgg gaatcagccg cgctgacggc gttcggcagc
     1381 ggaacgtggg aatacttcgg cgaaaccaac caggcgtcgc tgaacgattt catcaatacg
     1441 acgatgggcg ggattgcact cggcgagatg ttgcatcgca cggcgtggct gatcatcgat
     1501 ccgaccgcga ccggcggctc gcgcaagaag aaggagatct tcgcgacggc gatcgatccg
     1561 atgaacggga tcaaccggtt catttcgggc gacgcgcacc gcgtcagcga gaagccggcc
     1621 gagttcacgc cgtcctctct ggccgccacc acggccgccg gggtgctgtg gcgcggctcg
     1681 aacaccgcgg acgtcgaatc ggcggcctac ccgtcggtcc aggtcgatct cctgtatggg
     1741 gatccgacta cgggccgcag ccggacgcct tacgatgca
//
LOCUS       DATIMH010000437         6522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4144240, whole genome shotgun sequence.
ACCESSION   DATIMH010000437 DATIMH010000000
VERSION     DATIMH010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4144240"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VLI90_07450"
     CDS             complement(<1..1289)
                     /locus_tag="VLI90_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14078.1"
                     /translation="MGQMQKMARLFVLSVLLATGNICFGVTVVSVASFGAKADDRADD
                     TPAFVQALRACVLSPGSTLALDAGVYDVAMRSTPPDGSASPVLVISGARGLTIDGHGA
                     EIICHDFATLFALDGCRDVTIRNLRIDYDPLPFTAGQIVGEGDGFVDLKIEPHHPLQA
                     GLRAESLLPFDPVRKRMGQGTEDLYQPNLQRRTQKISPDVMRVPVTRPMYHPGDWVIL
                     RHQIYSKNALDFTNCDRVQFDDVTVYTCPGMALHAHGGSDFALHSFNVAIKPNSGRWF
                     TATADATHFNGVRGKVTLNDCLLESMGDDGANVHNWYLRVTQVLGPRRFKGVLGKDPS
                     WDPLLPMPGDHLEIGRSPNPLLPLINATVESAEYGDDGKAAVVTLTKTPSQPPRVDDV
                     VGNASTLPALEVHHCTIRRNRARGVLVQTRGAVVEQCT"
     gene            complement(1518..2681)
                     /locus_tag="VLI90_07455"
     CDS             complement(1518..2681)
                     /locus_tag="VLI90_07455"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HSV14079.1"
                     /translation="MRLTCPCLCLLLACCFALPARADLSVHVAAIDRDRVLKEADQYM
                     NEKPITITASHSPRSAGGEHDYFSEGDYWWPDPKNPDGPYIQRDGESNADNFNDHRLA
                     LIRLSIQVPGLVAAYKLTGDKKYADHAVDHLRAWFIDPATGMNPNLQYAQAIHGRYTG
                     RSTGIIDTLHLVEVARAVQVLKDKSGISQQDDAGVEQWFKEYTNWMLTSDNGKAEGRA
                     GNNHATCYWLQVAAFSSLIGNQDNLALCRTKYKEQLLPQMAPDGSFPAELKRTKPYSY
                     SNFNLDQMVALCQVLSTPQDDLWTFALPSGQTIRKGVEYMYPFVNDKSKWPKKPDVMF
                     YEFWPVRTMTWLLAGIHFNEPKYIDLWKKLESNPSNAEVIRNVPLRQPVLWVE"
     gene            2966..4687
                     /locus_tag="VLI90_07460"
     CDS             2966..4687
                     /locus_tag="VLI90_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14080.1"
                     /translation="MACFGAPGRRSFVGIFIGLSAATAMVGAGCDRSASQADQRVDQA
                     VSQALPQMNGSESDRNAAASLLTAANGETAASLPSRIRAKEALAQSELRIAEQMLPQI
                     ERTQSEVLRLAWEIEGLAGQVQASNQVASALAKYEPSQVQSALQKQTSDIKGTDEKSD
                     WVKTDAGSVPALSAVTRQQADLQARIADLQKTIQSLNDQRTQLLAKADQLNQDSSKQK
                     GDKAVEIFKQSADARKQADDLVVQIDQNTEQLSRAQADLAVRNGQAAVLNDAIATIDK
                     HGQDVTNAWQTLQQQMEAQHKQAKDLLGDESKPPAPEDTTGGATIAAKGLWLEELNKA
                     NRTRRDDAESHFNSAIGQFKDAANLAEELQRDLQQKINDPKNQNRPDATAWRDLSTAL
                     HPARFQMEQANAEVHRAQLLAGRAAEANARLQLAAHLKPILAEAKLQMPASFQDADNK
                     VADDLKNAREQADASYKDASELLGTIAEGTAENLRQTAHIAKIFADYNWYLLSAAQGD
                     KDAATKHLQSAQAERDVVVQGGVTPRNLPAELAVAPTTAPAAPAAPGAPTETPAAPAA
                     PAPAPQG"
     gene            4792..5127
                     /locus_tag="VLI90_07465"
     CDS             4792..5127
                     /locus_tag="VLI90_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HSV14081.1"
                     /translation="MHANNAWATCELCQRDVPRSMITLHHLTPRERGGTADDRSPLCK
                     PCHKQLHAMFGNRQLERDYSTIEALRKAEALQPFLKWIRKQKSGRNFRTITSLAHPGS
                     KRNRRGRRG"
     gene            complement(5230..5700)
                     /locus_tag="VLI90_07470"
     CDS             complement(5230..5700)
                     /locus_tag="VLI90_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14082.1"
                     /translation="MSERTFEPDDLDATLRRAMRHRAEPVAPSDLAGRAMRRAQAADA
                     LAAVARVNRLNRLFTAIAAVVIICVAGWIFRARLSAGGFQPWSDTSTTSTDSTDTSTT
                     STTTTLATATQWMYVGIGLAVTAIGVLAVQRTVGGADEWVLRWNATGTRRANAV"
     gene            complement(5697..6239)
                     /locus_tag="VLI90_07475"
     CDS             complement(5697..6239)
                     /locus_tag="VLI90_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSV14083.1"
                     /translation="MDLERLKQQDARAFAELVAEHQAIILGLGQSLGLRGPDLDDAAA
                     EAFAAVYRALPRFEARAQLGTWVYRIAYRVIVKARTKRRRHATVELPDEPSATPAAGD
                     AGPMQRAEQAETAVAVWSAVESLDERSATAVELYYRREWPLEKIADVLECPVGTVKTL
                     LYRARERLRTLLSSQQEISP"
     gene            6343..>6522
                     /locus_tag="VLI90_07480"
     CDS             6343..>6522
                     /locus_tag="VLI90_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14084.1"
                     /translation="MPLFAMSRGYSSRTRRITVINTLLDPMAYPAAEIAQLYARRWEV
                     QTCFRHLKQTLDLEHL"
BASE COUNT         1162 a         2182 c         2086 g         1092 t
ORIGIN      
        1 gtgcattgct cgacgaccgc gccgcgcgtc tgaacgagca cgccgcgcgc gcggttgcgg
       61 cggatcgtgc agtgatgcac ctccagcgcc ggcaacgtgc tggcattgcc gacgacgtca
      121 tccacgcgcg gcggttgcga tggcgttttg gtcagcgtca cgacggccgc cttgccgtcg
      181 tctccgtatt ccgccgattc gaccgtcgcg ttgatcagcg gcaacagcgg attcggcgac
      241 ctgccgattt ccaagtgatc gcccggcatc ggcagcagcg ggtcccagct gggatccttg
      301 cccagcacac ccttgaagcg gcgcgggccg agcacctgcg tgacgcgcag ataccagttg
      361 tggacgttcg ccccgtcgtc gcccatcgat tccagcaggc agtcgttcag cgtgaccttg
      421 ccgcgcacgc cgttgaaatg cgtggcatcg gcggtggcgg tgaaccatcg cccgctgttt
      481 ggtttgatcg cgacgttgaa cgagtgtagc gcgaagtcgg acccgccgtg tgcgtgcagc
      541 gccatgccgg ggcaggtgta gacggtgaca tcgtcgaact gcacgcgatc gcaattggta
      601 aagtcgagcg cgttcttcga gtagatctgg tgccgcagga tgacccagtc gccggggtga
      661 tacatcgggc gcgttaccgg cacgcgcatc acgtcgggcg agatcttctg cgtgcgtcgc
      721 tgcagattcg gctgatacag atcttcggtc ccctggccca tccgcttgcg caccggatcg
      781 aacggcagca ggctttcggc gcgcaggccc gcctgcagcg gatggtgcgg ttcgatcttc
      841 agatcgacga agccgtcgcc ttcaccgacg atttgcccgg ccgtgaacgg caggggatcg
      901 tagtcgattc gcaagttgcg gatcgtcacg tcgcggcaac cgtccagcgc gaacagcgtg
      961 gcgaaatcgt ggcagatgat ctcggccccg tgaccgtcga tcgtcagccc gcgggcgccg
     1021 ctgatgacga ggactggcga tgccgacccg tccggcggcg tgctccgcat cgccacgtca
     1081 tacacacccg cgtccagcgc gagcgtcgat cccggcgaca gcacgcacgc ccgcagcgct
     1141 tggacgaacg cgggcgtgtc gtccgcgcga tcgtcggcct tggcgccgaa gctcgcaacg
     1201 gaaacgacgg tgacgccaaa acagatgttg ccagtggcga gcaacaccga tagcacgaac
     1261 aaccgcgcca tcttctgcat ctgacccatc tcgctacctt ttttcggaga atcctcgcac
     1321 catcacactg atcgttccgc actgaccgaa gatcacgagc gggcatgaac tttgtaacac
     1381 gcgggagtgc gcacgctcgc cgtcatttcg ctcgccgttg ccgcgttcgc cgctgcgacg
     1441 cctgcctcgc cctgcttttt tgacggcaaa gaggcgacgc aggcgtcgca gctacaagtg
     1501 tgctgtaccc tcgccgatca ctccacccac agcacgggct ggcgcagcgg gacgttgcgg
     1561 atcacctccg cgttgctcgg gttcgactcc agcttcttcc acagatcgat gtacttcggc
     1621 tcattgaaat ggatgccggc cagcagccag gtcatcgtgc gcacgggcca gaactcgtag
     1681 aacatcacgt ccggtttttt cggccacttc gatttgtcgt tcacgaacgg atacatgtac
     1741 tcgacgccct tgcggatcgt ctgcccgctc ggcagcgcga acgtccacag gtcatcctgc
     1801 ggcgtcgata gcacctggca cagcgcgacc atctgatcca ggttgaagtt cgaataactg
     1861 tatggcttgg tgcgcttcag ctccgcgggg aagctgccgt cgggggccat ctgcggcagc
     1921 agctgctcct tgtacttggt gcggcacagc gcgaggttgt cctggttgcc gatgagcgat
     1981 gagaacgccg ccacctgcag ccaatagcac gtcgcgtggt tgttcccggc gcggccttcg
     2041 gccttaccgt tgtcgctggt caacatccag ttggtgtatt ccttgaacca ctgctcgacc
     2101 ccggcgtcgt cctgctggct gatgccggat ttgtccttca gcacctggac cgcgcgggcg
     2161 acttcgacca ggtgcagcgt gtcgatgatg ccggtggacc ggccggtgta tcggccgtgg
     2221 atcgcctggg cgtattgcag gttcgggttc atccccgtcg ccgggtcgat gaaccaggcg
     2281 cgaagatgat cgacggcgtg gtcggcatat ttcttgtcgc ccgtcagctt gtacgccgcc
     2341 accagccctg gcacctggat gctcaggcgg atcagcgcca gtcgatggtc gttgaagttg
     2401 tcggcgttcg attcgccgtc gcgctggatg tacgggccgt cggggttctt cgggtccggc
     2461 caccagtaat cgccttcgga aaagtaatca tgctcgccgc cagcgcttcg cggcgagtgc
     2521 gacgcggtga tggtgatcgg cttttcgttc atgtactggt cggcttcctt cagcacgcga
     2581 tcgcgatcga tcgcggccac gtgcaccgag agatcagcgc gcgcgggcag cgcaaagcag
     2641 caggccagca acaggcacag gcacgggcag gtcagtcgca tgggcgggtc ctccttcgag
     2701 gcacgggttg aacggcacgt cgttcctcaa taccaccttc gggcggggac gttcaattta
     2761 tccggtcccc tctcccggta ctccgtcggt actccgggag agggggaagg agcgacctgc
     2821 aatcgcccca gggcgcgcat gtcacgcccg tggcggtagt ttgacttcgt tcgcctgcct
     2881 gccgacaatt caccccattg cggggcggga gttggcgcgg cggcgcgcat caacagttgc
     2941 agtgacccac cggagacgtt ttccaatggc ttgtttcggc gcacctggac gtcgttcctt
     3001 cgtcggcatc ttcatcggtc tctccgctgc caccgcaatg gtcggcgccg gttgcgatcg
     3061 gtccgccagc caggcggacc aacgcgtgga ccaggcggtc agccaggcct tgccgcagat
     3121 gaacggatcc gagtccgatc gcaacgcggc ggcgtcgctg ttgacggccg ccaacggtga
     3181 gaccgcggcg tcgctgccca gtcgcattcg cgccaaggaa gcgctggccc aaagcgagct
     3241 gcgcatcgcc gagcagatgc tgccgcagat cgagcggacc cagagcgaag tgctgcgact
     3301 ggcgtgggag atcgaaggcc tggccggcca ggtgcaggcg agcaaccagg tcgcgtccgc
     3361 gctggcgaag tatgagcctt cgcaggtgca gagcgcgctg cagaaacaga cgagcgacat
     3421 caagggcacc gatgagaagt ccgactgggt gaagaccgac gccggctcgg tgccggcgct
     3481 gtcggccgtc accaggcagc aggcggatct tcaggcgcgc atcgccgatt tgcagaagac
     3541 catccagtcg ctgaacgatc agcgcaccca gctgctggcc aaggccgatc aactcaatca
     3601 ggactcgagc aagcagaagg gcgacaaggc ggtcgagatc ttcaagcagt cggccgacgc
     3661 gcgcaagcag gcggacgacc tggtcgtgca gatcgatcag aacaccgagc agctgtcgcg
     3721 cgcccaggcg gacctcgccg tgcgcaacgg gcaggcggcg gtgttgaacg atgcgatcgc
     3781 caccatcgac aagcacgggc aggacgtgac caacgcctgg cagacgcttc aacagcagat
     3841 ggaagcccag cacaagcagg cgaaggattt gctcggcgac gagagcaaac caccggcgcc
     3901 ggaagacaca acgggcggcg cgacgatcgc cgccaagggg ttgtggctgg aagagctgaa
     3961 caaggcgaac cgcacccgcc gggacgatgc cgagtcgcac ttcaacagcg cgatcggcca
     4021 gttcaaagac gcggccaacc tggccgagga actgcagcgc gacctgcagc agaagataaa
     4081 cgatccgaag aaccaaaacc gccccgacgc cacagcgtgg cgcgatttgt ccaccgcgct
     4141 gcatccggcg cggttccaga tggaacaggc gaacgccgag gtacaccgcg cccagttgct
     4201 cgccggtcgc gctgccgagg cgaacgctcg attgcagttg gcggcccatc tgaagcccat
     4261 cctggccgag gcgaagttgc agatgccggc gtcgttccag gacgcggaca acaaggtggc
     4321 cgacgatctg aagaacgccc gcgagcaggc ggatgcttcg tacaaggatg ccagcgaact
     4381 gctcggcacg atcgccgaag gcaccgcgga aaacctgcgg cagacggcgc acatcgccaa
     4441 gatcttcgcg gattacaatt ggtatctgct gtcggccgcc caaggcgaca aggacgcggc
     4501 taccaagcat ctccaatcgg cccaagccga gcgcgacgtg gtggtgcaag gcggagtgac
     4561 gccgaggaac ctgccagcgg agctggcggt ggcgccgacg accgcgccgg ccgcgcccgc
     4621 cgcgccggga gcgcccactg agactccggc cgcgcctgct gcgcccgcgc ccgctccaca
     4681 aggctagtcc ttcgcgcacc gtcccacggc agagccgtgg gctttggtga tcaatctcat
     4741 cccataaaaa agcccacggc tccgccgtgg gaccgcgcgc taagatgcgg catgcacgcc
     4801 aacaatgcct gggccacatg tgaactgtgc cagcgcgacg tcccgcgctc gatgatcacg
     4861 ctgcatcacc tcacgccgcg cgagcgtggc ggcaccgccg acgatcgcag cccgctgtgc
     4921 aagccgtgcc acaagcaact gcacgcgatg ttcggcaatc ggcagttgga gcgcgattac
     4981 tcgacgatcg aagcgctaag aaaggccgag gcgctccagc cgttcttgaa gtggattcga
     5041 aagcagaagt cgggccgcaa cttccgcacg atcacgtcgc tggcgcatcc cggatcgaag
     5101 cgcaaccggc gcggcagacg cggatgaccg caacgtcccc cgcgtagcgg tagccccgca
     5161 gggacacgtg tttggtcttg gagaggaaaa cgagtgcccc ggcggggcta ccgctacgcg
     5221 ggcagaggat cacaccgcgt tcgcgcgcct cgtccccgtc gcgttccagc gcagcaccca
     5281 ctcatccgcc ccgcccacgg tgcgttgcac cgcgaggacg ccgattgccg tcaccgccag
     5341 tccgatgccg acgtacatcc actgcgtcgc cgtcgccagc gttgtcgtcg ttgatgtcgt
     5401 tgatgtgtcc gtgctgtccg tgctcgtcgt gctggtatcc gaccacggct gaaacccgcc
     5461 cgcggacagc cttgctcgga agatccaccc ggcgacgcag ataatcacca ccgccgcgat
     5521 ggcggtgaac aggcggttca ggcggttcac ccgcgcgacc gccgccagcg cgtcggcggc
     5581 ttgcgcgcgg cgcatcgccc gccccgccaa atcgcttggc gcgaccggct cggcgcgatg
     5641 ccgcatcgca cgccgcagcg tcgcatcgag atcatcgggt tcgaacgtcc gctcgctcac
     5701 gggcttatct cctgctggct cgacaacagc gtccgcaatc gctcgcgggc gcggtacagc
     5761 aatgtcttga ccgtgcccac cgggcactcc agcacgtccg ctatcttctc cagcggccac
     5821 tcccggcgat agtacaattc caccgccgtc gccgaccgct catccagcga ctcgaccgct
     5881 gaccaaacgg ccaccgcggt ctccgcttgc tcggcgcgct gcattggtcc ggcatcgccc
     5941 gccgcggggg tggccgacgg ttcatccggc agctccaccg tcgcatgccg gcgccgcttg
     6001 gttcgggcct tgacgatcac gcgatacgcg atgcgataga cccaggtgcc cagttgagcc
     6061 cgggcttcga accgcggcag cgcgcgatag acggccgcaa acgcctcggc cgccgcgtcg
     6121 tccaggtcgg ggccgcgcag gcccagcgac tgcccgagtc ccaaaatgat cgcctggtgc
     6181 tcggcgacca gctcggcaaa ggcgcgtgcg tcctgctgtt taagccgctc cagatccaag
     6241 ccgccgcctc acttgcgcgc cgcatccatt tgtgctgaca ctacgttagt gcccgatgga
     6301 tctggcgcgg tttcagcaat ccccagcgca ggttagagcg gcgtgcccct ttttgctatg
     6361 agcagggggt atagctcaag aacccgccgg attaccgtca tcaacacgct gctggacccg
     6421 atggcctatc cggccgcgga gatcgcgcag ctgtacgcgc ggcgatggga agtccagacc
     6481 tgctttcgcc acctcaagca gacgctggac ctggagcacc tg
//
LOCUS       DATIMH010000438         5027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_683300, whole genome shotgun sequence.
ACCESSION   DATIMH010000438 DATIMH010000000
VERSION     DATIMH010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683300"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VLI90_07485"
     CDS             complement(<1..907)
                     /locus_tag="VLI90_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HSV14085.1"
                     /translation="MRDLLDILAAVAALDSNDGSAVLATVVRVEGSSYRLPGARMLIE
                     ANGARTGSVSGGCLEADIARRGRLLTIERPRELVRYDGSDEDVAWGFGLGCNGSIEVL
                     IERLDAEPRAGLAFIRHCVEQRAGGTLVTIFRADGDVPFHAGERFNSADDIPSLFGND
                     SRVTTTVTCETTTGGRVQALVEAIHPPLPLVIFGAGHDAAPLVRAAKQLGWHVTIADR
                     RAGYARSDRFPLADAVVACDIDAMADRIRVTSDTAAVVMTHHFPDDQVLIPMLLRSPA
                     RYIGVLGPRARTERMLAQAQYSNDQL"
     gene            complement(1267..1698)
                     /locus_tag="VLI90_07490"
     CDS             complement(1267..1698)
                     /locus_tag="VLI90_07490"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSV14086.1"
                     /translation="MPVRQSEAEWSGDLKGGKGKMRLGSGAYEGGYSFQSRMENGAGT
                     NPEELIAAAHAGCFSMALSAKLAGAGHVPTRVHTTAQVDFGQVAGGFAIQKITLNTDA
                     DVPGIDDATFQRIANEAKEGCPVSKALAAVPSIVLNAKLKK"
     gene            complement(1879..3252)
                     /locus_tag="VLI90_07495"
     CDS             complement(1879..3252)
                     /locus_tag="VLI90_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HSV14087.1"
                     /translation="MSQPLADRIAEYAVNLRFDQLSPEVVHEVKRRVLDSLGCAFGAW
                     HDEPGVVARRVASALSAERGSTLIGTAHRAPGDWAAFANGTIVRYLDYNDTYLSKEPA
                     HPSDNIPAAMAIAESVGASGRELITAIALAYEIQCRLCDAVSLRAIGWDHVTYGCFSV
                     AAAAGLLMKLDATKIRHALGIAGVTSAALRQTRVGELSHWKGCAFANAARHGVYAAML
                     AAAGMTGPSPIFEGEKGFEKLVSREAIKLAGPIVGERDELMILRTSIKCWPVEYHAQS
                     AVEAAVALRQQLGDPSQIESVLIESHDAAVDIIGSEPEKWRPKSRETADHSLPYITAA
                     ALYDGDVTLATFSAKRIADAKLIDFVQKVKVVRHNELSAMYPGAVGNIVTVKLRDGRT
                     LTKRVDHACGHAKNRMSDGQVRAKFHRLADPVIGAQRANGIVTWVEKLDVAPRIDELF
                     PLLAVSS"
     gene            complement(3357..3430)
                     /locus_tag="VLI90_07500"
     tRNA            complement(3357..3430)
                     /locus_tag="VLI90_07500"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3394..3396),aa:Ile,seq:gat)
     gene            3892..4113
                     /locus_tag="VLI90_07505"
     CDS             3892..4113
                     /locus_tag="VLI90_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14088.1"
                     /translation="MMIIGSLLIVPSLPLFLYDLLWHQLALLWPTFIPINLLVISLRT
                     LRWGYMELQNLRSQERAAALAQAAAAKQP"
     gene            4196..4894
                     /locus_tag="VLI90_07510"
     CDS             4196..4894
                     /locus_tag="VLI90_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HSV14089.1"
                     /translation="MAATFSVLVLTAPPPGLGSEGGGAFVKIDGREALLRSVELFLNR
                     DNIKQIQLAILPDEMEEAKRKYGAHLGFSGVKLINGGPRWVDQLSAAAATISPDATHV
                     IVHDAARPAVPYSDIDALLAEAEQHPAVALTTPLRTTIIETDEGGNPVGSYPPQRYVQ
                     LLTPQAFSKAKFAEIAAAKAEVHPSQWKLIKGSPLNIRAGGPGEAGLIKTMINMLPKP
                     KLKAPSSPFEEAQW"
BASE COUNT          876 a         1639 c         1629 g          883 t
ORIGIN      
        1 cgagttggtc gttgctgtat tgagcctgag cgagcatgcg ctcggtgcgc gcccgcgggc
       61 cgagcacacc gatgtacctg gccggtgacc gcagcagcat cgggatcagc acctggtcgt
      121 cggggaaatg gtgcgtcatc accacggcag cggtgtcgga cgtgacgcgg atgcgatcgg
      181 ccatcgcgtc gatgtcgcag gcgacaaccg cgtcggccag cgggaagcga tcgctgcggg
      241 cgtatcccgc gcgacggtcg gcgatcgtca cgtgccagcc cagttgcttg gccgcgcgca
      301 cgagcggagc ggcatcgtgc ccagcgccga agatgaccag cggcagcggt ggatggatcg
      361 cctcgaccag cgcctgcaca cgcccgccgg tggtggtttc acaggtgacg gtcgtcgtga
      421 cgcggctgtc gttgccgaag agcgatggga tgtcgtcggc gctgttgaag cgttcgccgg
      481 catgaaacgg cacgtcacca tcggcgcgga agatcgtcac gagcgtccca ccggcgcgct
      541 gctccacgca gtggcggatg aacgccagcc cagcccgcgg ctccgcgtcg aggcgttcga
      601 tcaggacttc gattgaaccg ttgcagccca ggccgaatcc ccacgcgacg tcttcatcgg
      661 agccgtcgta gcgcaccagc tcgcgcgggc gttcgatcgt caacaaccgc ccgcggcggg
      721 cgatgtcggc ttccaggcaa ccgccgctga ccgaaccggt gcgcgcgccg ttcgcttcga
      781 tcaacatccg agcgccgggg aggcgatacg aggagccttc gacgcgcacg acggtggcga
      841 ggacggcgga gccgtcattg gaatcgagcg cggcgacggc ggcgaggatg tccagcaagt
      901 cgcgcatgcg tcactacatg atatccgcgc agaagctcga tgttgtccat tgcactcgcg
      961 cagtcgcagc cgcgagattt tagcccgcgc ggacggttcg cgcgtcataa acctgctcgg
     1021 tattcgaacg atccgcgggc taaagccttg cggcggcgat tgggttcact tttcacgatg
     1081 aacccggtca tcccccgcag cacctcttgt acttcctccc actaccgcag gggcatggtt
     1141 cgttgcggcc gaccttgagc gcggcgagca ggcatcacaa tttcgtctgg aaccagcgcc
     1201 gcccgcggct taacgtctgt tcgatcgacg gatgaaaaca cgcgaagccg caagcggcgg
     1261 gaaagatcac ttcttcaact tcgcgttcaa cacgatcgac ggcaccgcag cgagcgcttt
     1321 ggagacgggg cagccttcct tcgcttcgtt ggcgatccgc tgaaacgtcg cgtcatcaat
     1381 gccgggcacg tcggcgtcgg tgttcagggt gatcttctga atggcgaatc cgccggcgac
     1441 ttgtccgaaa tccacctgcg ccgtcgtgtg cacgcgcgtc ggcacgtgcc ccgcgccggc
     1501 gagcttggcg ctgagggcca tcgagaaaca ccccgcgtgc gccgcggcga tgagttcctc
     1561 ggggttcgtc ccagcgccgt tttccatgcg cgactggaac gagtaaccgc cttcgtaagc
     1621 gccgctgccg agccgcatct tgcctttgcc gcctttgagg tcgccggacc attccgcttc
     1681 ggattgacga acgggcattg tgggtctcct tctcgagttg cgggcgtgca cgcacgccct
     1741 tcggggacag attttcgcgc acatgcgcgc cggtgactat agcagtggga cgttgccgct
     1801 gtgaagtcca ggggcaagcg cacttggaga atgcgtgctc tttccccctg ctgtgaacgc
     1861 ggcttcacaa gcgcagtttc acgacgacac cgcaagcagc gggaacagct cgtcgatccg
     1921 cggggctacg tccagcttct ccacccacgt gacgatcccg ttcgcgcgct gcgcgccgat
     1981 caccggatcc gccagccggt ggaacttcgc ccgcacctgc ccgtcgctca tgcggttctt
     2041 cgcgtggccg caggcgtggt ccacgcgctt ggtcagcgtc cggccgtcgc gcagcttgac
     2101 ggtgacgata ttgcctaccg cgccggggta catcgcgctc agttcattgt gccgcacgac
     2161 tttcaccttt tgaacgaagt cgatcagctt cgcatccgcg atgcgcttgg cgctgaacgt
     2221 cgccagcgtc acgtcgccgt cgtacaacgc ggcggcggtg atgtacggca ggctgtggtc
     2281 ggccgtctcg cgcgacttcg gccgccattt ttccggctcg ctgccgatga tgtccaccgc
     2341 cgcgtcgtgg ctttcgatca gcaccgactc gatctgcgac ggatctccaa gctgctgccg
     2401 caacgcgacc gcggcttcga cggcgctttg cgcgtgatat tcgaccggcc agcacttgat
     2461 gctcgtgcgc aggatcatca gctcgtcgcg ctcgcccacg atcggcccgg ccagcttgat
     2521 cgcctcgcgg ctgacgagct tctcaaaacc cttctcgcct tcgaagatgg gggaaggccc
     2581 cgtcatcccc gcggcggcga gcatggcggc ataaacgcca tgccgcgcgg cgttcgcgaa
     2641 ggcgcagccc ttccagtgcg acagttctcc aacgcgcgtt tgccgcagcg cagcgctggt
     2701 cacgccggcg atgccgagcg cgtggcggat cttcgtcgcg tccagcttca tcagcagccc
     2761 ggccgcggcg gcgaccgaga agcagccgta ggtgacgtgg tcccaaccga tcgcccgcag
     2821 gctgacggcg tcgcacaggc ggcattgaat ttcgtacgcc agcgcaatcg cggtgatcaa
     2881 ttctcgacca ctcgcgccga cgctttcagc gatcgccatc gccgcgggga tgttgtcgct
     2941 cgggtgcgcc ggctccttcg acaggtacgt gtcgttgtag tcgaggtaac ggacgatggt
     3001 cccgttggcg aacgcggccc agtcgccggg ggcgcgatga gcggtgccga ttagggtgga
     3061 tccgcgctcg gccgacaatg ccgatgcgac acgacgggcg acgacgccgg gctcgtcatg
     3121 ccacgcgccg aacgcgcagc cgagcgagtc gagcacgcgc cgtttcactt catgaaccac
     3181 ctccggcgac agctggtcga accgaaggtt gacggcgtat tcggcgatgc ggtcggcaag
     3241 cggttgggac atgaggatca atcctgtttg agcgacggac ttgcggggca agcgcgggcg
     3301 caacgaaaaa ccgccgcacg tttgacgccg gcggcggttt agcatgcttg ccgatatggg
     3361 cccgacacga ttcgaacgtg cgacctcgtc cttatcaggg acgcgctcta accaactgag
     3421 ctacgagccc gcgttttcac caccgccatc acgggccagc ttcaacgcct tcccaacggc
     3481 aggcgaagaa ttgtaaggcg agcgcgagtc gtgtcaaccg gtggttgtgc gtggttgtgc
     3541 gtcgtgttgt gcgtcgtcgt ccaacgacca cccctgcaat cccaccgtcg cggctggtac
     3601 aaccccgtcc gtagattgga attcacgatt tcgggaactc ccttttagcg gcggcccgaa
     3661 gggccgagcg ttcgtcgcag taaatttcat caatcgcgat ctcgttgtct cgacaggagc
     3721 accggttgaa ggaacagcgg ttgtgtgacc gaaagcgccg tcctcccgac ctggcggacg
     3781 cctgtccccc ggccaactca cagtatttcc gagcaaagcg gcggaaccgc tcatcagctt
     3841 cgtcgacccg cgacacgccg gcacgcgccc agccgcgcga aaagccgccg gatgatgatc
     3901 atcggctcgc tcctgatcgt gcccagcctt ccgctgtttc tgtacgatct gctctggcat
     3961 caactggcgt tgctttggcc gacgttcatc ccgatcaacc tgttggtcat cagtctgcga
     4021 accctgcgct ggggctacat ggaactccaa aacctgcggt cccaggagcg ggcggccgcg
     4081 ctggcgcagg cggcagccgc gaagcaacct tgacagtcgc cggttgcagg cgaacagtta
     4141 gcccgcgatc gctcgcgcgc gagcgacaca cggcaacgac aacggagatg caatcatggc
     4201 cgcgacattc agcgtactgg tcctcaccgc cccgccgccg gggttgggca gtgaaggcgg
     4261 cggcgctttc gtgaagatcg acggccgcga agccctgctg cgcagcgtcg agctgttcct
     4321 caatcgcgac aacatcaagc agatccagtt ggcgatcctt ccagacgaga tggaagaggc
     4381 caagcgaaag tacggcgcgc acctgggctt ctccggcgtc aagctgatca acggcggtcc
     4441 gcgctgggtg gaccaattga gcgccgcggc ggcgacgatt tcgccggatg cgacgcacgt
     4501 gatcgtgcac gacgccgcgc gccccgcggt tccgtattcg gatatcgatg cgctgctcgc
     4561 cgaagccgag cagcacccgg cggtggcgct gaccacgccg ctgcggacaa cgatcatcga
     4621 gactgacgaa ggcggcaacc ccgtcgggtc atatccaccg cagcgatacg tgcagctgct
     4681 gacgccacag gcgttcagca aggcgaagtt cgcggagatc gccgcggcga aggcggaggt
     4741 tcatccatcg caatggaaac tcatcaaagg gtcgccgctg aacatccgcg ccggcggacc
     4801 cggcgaagcg gggctgatca agacgatgat caacatgctg cccaagccga agctgaaggc
     4861 gccgagcagc ccatttgaag aggcgcagtg gtgaacgcga tggacgtgcg ggtggggagg
     4921 attgtgtcga ttgtgcggac gccatgtgca gggaatctga atgcgaagct cgaatgacga
     4981 acgcgcgtgc gggcgtcgcc cggactaacc gcttcagcgg ttagtgc
//
LOCUS       DATIMH010000439         7575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_103763, whole genome shotgun sequence.
ACCESSION   DATIMH010000439 DATIMH010000000
VERSION     DATIMH010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103763"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VLI90_07515"
     CDS             complement(<1..233)
                     /locus_tag="VLI90_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HSV14090.1"
                     /translation="MIRIPTNRPPSHPGEMLKEEFLSPLGWTQRELAARLRVSYPRLN
                     DLIHGRRGVTTDTALRLERLLGVEAQFWLNLQLA"
     gene            complement(242..535)
                     /locus_tag="VLI90_07520"
     CDS             complement(242..535)
                     /locus_tag="VLI90_07520"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSV14091.1"
                     /translation="MIASFADPGTQDVFNGLASKAARRACPKDLWNVARRQLDQLNAA
                     SRIDTLRVPPGNRFEALKGERVGQHSIRINEQFRVCLRWTDAGAESVAIVDYH"
     gene            658..2433
                     /gene="polX"
                     /locus_tag="VLI90_07525"
     CDS             658..2433
                     /gene="polX"
                     /locus_tag="VLI90_07525"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HSV14092.1"
                     /translation="MALNHDLSELFRQMAAIMDIKGESVFKSIAFSKVSRVLKDMTFD
                     IRKCCDDGTLKEIEGIGESSRKIILEYVKTGRSTDYEELAASIPAGLIPMLEIPGLGP
                     KTIALFWKQRGVTSLEELTKAIDEGKLDGIKGVGEKKIMAIKQGIQLRSQAVGRMGII
                     DAMPIGESLVARLRELKHVKAVEVAGSLRRRRETVGDLDLVCSLKDGVDGTAVTAAFV
                     EFPEVERILGQGNTKASVLTVGGLQVDLRIVPAENFGAALLYFTGSKDHNVKLRGLAL
                     DKEMTLNEWGLYKLAEHEKAEKKTGEPPRCKPVASKTEADVYKKLGMAYVEPEMREDR
                     GEVAAALADKLPKLIQLSDIRGDLHTHTTASDGTASIAEMAEAAIARGYAFLGVTDHS
                     KSQVIANGLTAERLLKHVKEIHKVNDRYKGQIRLFAGCEVDILADGRLDFEDAVLKEL
                     DFVVASPHISLKQDERKATDRLKRALDSRYVNVIGHPTGRLINSREGLPVNFGELFPL
                     AAKNGTAMEINAGYPRLDLNEINARAAINAGVKLSIDTDAHGTGGFDEIVFGINVARR
                     AWVTKADVINCMTLAELEAFVARKR"
     gene            complement(2681..3559)
                     /gene="galU"
                     /locus_tag="VLI90_07530"
     CDS             complement(2681..3559)
                     /gene="galU"
                     /locus_tag="VLI90_07530"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HSV14093.1"
                     /translation="MTHRPLRKAVIPAAGFGTRMLPAAKAVPKELLPILDRPTIQYVI
                     EEAAAGGIEDVLLVTSRDKRAIEDHFDRNAELEQRLKAGGKESLLASVNELAARVKIH
                     SVRQPEQRGLGDAVAQARKHVGDEPFICLLGDTIFSGGDEGPTAQLAAAYREFGSSVI
                     GLEEVPLERVSRYGIVGGAMLRDGVMKLDTLVEKPSRENAPSRFAIAARYVLTPAIFQ
                     CLASTSPGKGGEIQLTDALRALLAREPIYGVVLKSRRHDIGNPIDWLRTNLMFAARDP
                     AMWEQIAPLARELLSR"
     gene            complement(4039..5466)
                     /locus_tag="VLI90_07535"
     CDS             complement(4039..5466)
                     /locus_tag="VLI90_07535"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV14094.1"
                     /translation="MAEPAPLAAPSSSPTTGGAAAGIFRALRHRNYRLFFGGQLVSLV
                     GTFLTQIATVWFVYRLTKDTRLLGIVGFAGQLPMFLLAPFAGVWADRVNRQRFIVLTQ
                     ALSMLQSFGLAAVAFYFGRPGHINTQVAVPSLIGLAIIQGLVNAFDMPARQAFLVEMV
                     TDRADLANAIALNSTMVHGARLIGPAAAGLIIAAVGESLCFLIDAISYIGVIIALVAM
                     RVTPRPPRAPKSVLHELAEGAKYVWHFRPIRVLLLLMAMLSLTGMPAISTLMPIFADH
                     FSTGGASRNGAQVFGFLGTMSGLGALAGAIYLASRKTVVGLGRLIAIAALVFGLGAIA
                     FASSNLLWLSLLIIPIAGWGMITCFASANTILQTLAEDDKRGRVMSFFTMAFVGMAPF
                     GSLLTGYAAYHLTPAIGGDAFRGASRTILIAGVICVIAAVSYASKLPAMRAIVRPIYI
                     SKGILPELAKGLQTADTMPGATGEQ"
     gene            complement(5644..5886)
                     /locus_tag="VLI90_07540"
     CDS             complement(5644..5886)
                     /locus_tag="VLI90_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14095.1"
                     /translation="MAIVTRRITPATDFLAGLINIEYLTPQDIRDAWHVFERYLDKDW
                     SFTDCTSKVVMERLGITTAFAFDQHFRQFGTVTVVP"
     gene            complement(6034..6276)
                     /locus_tag="VLI90_07545"
     CDS             complement(6034..6276)
                     /locus_tag="VLI90_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HSV14096.1"
                     /translation="MTKIGVIFDKGVFRPTEPVALPEGTKAVVEVDRDAAEMSAEELK
                     TLTEWIDRVQASARLKGIPDDGGTSHDKYLYGEDPS"
     gene            complement(6323..7273)
                     /locus_tag="VLI90_07550"
     CDS             complement(6323..7273)
                     /locus_tag="VLI90_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14097.1"
                     /translation="MSAGSPNSTDVKMLPDAATSPPPPPPPPVVVAPAPAPTPSVVST
                     AIASTSAPSRQLSELPKDELESLAQDFGLDPTRYKTRQHLVAALHERRQLIAGLDRDA
                     MIDVVRWGRRPVTVNASKEQIAQEIARIRSMKFSGLSHRGLIVLAMLRGVAIRENDSV
                     PALIRKLRRQEGFFSKINRKRRAALGSLVSKMVGEDEAEREYQFLPPPASGPTAGPTA
                     PPPPRPSSLKEEIEESGLVGGLTNRIKKTADSYLNQKLDEIESRIDRKLDEIDRRLAE
                     WRDKEIANRIRILKITLWASVIVGAFSLVYSYVKVYLVGH"
     gene            complement(7251..>7575)
                     /locus_tag="VLI90_07555"
     CDS             complement(7251..>7575)
                     /locus_tag="VLI90_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14098.1"
                     /translation="FFRYALGWVVTIYATVITLQSLWGWYVWLAGSDKYISLVRRYVI
                     VHGLRLRFRAFWGDVIISVLLCVAFLILWHAHTIIYRFAADEKSVRESLSHNVQPRHV
                     SRITQ"
BASE COUNT         1511 a         2322 c         2401 g         1341 t
ORIGIN      
        1 gcgagttgca ggttcagcca aaactgcgcc tcgaccccca gcagccgctc cagtcgcagc
       61 gccgtgtccg tcgtcacacc acggcggcca tgaatcaggt cgttcagccg cggatacgaa
      121 actcgaagtc gcgccgccag ctcgcgctgg gtccaaccca agggcgagag aaactcctct
      181 ttcagcatct cgcccggatg cgacggaggt cggttagtgg gaattcgaat catatcagac
      241 ctcaatggta atcaacgatc gccaccgatt cggcccccgc gtcagtccac cgaaggcaga
      301 cccggaactg ctcgttgatt cgaattgaat gttgacccac tcgctcacct ttcagggctt
      361 cgaaccgatt cccggggggc acccgcagag tgtcgatgcg actggctgca ttcagttggt
      421 caagctgtcg ccgtgccacg ttccatagat ctttcgggca ggctcgacgc gctgctttgg
      481 aagccagacc gttgaagacg tcttgcgtgc ccggatcggc aaacgatgcg atcacaacgt
      541 gaattataac ggatcatatt atatgtgaca atcgcaatct cgagttaacc aaagctgcgc
      601 cggacgaggg agaccagtga gatgccaatg tcgggcgcga cgaggtaaac ttcctccatg
      661 gcccttaacc acgacctctc ggaactcttc cgccagatgg cggccatcat ggacatcaag
      721 ggcgaaagcg tcttcaagtc catcgccttc tcgaaagtct cgcgggtgct gaaggacatg
      781 accttcgaca tccgcaaatg ttgcgatgac ggcacgctca aggaaatcga aggcatcggc
      841 gaatcgagcc ggaagatcat tctcgaatac gtgaagaccg gccgcagtac ggactacgaa
      901 gagctggccg cgagcatccc ggcggggctg attccgatgc tggagattcc gggactgggg
      961 ccgaagacga tcgcgctctt ctggaaacag cgcggcgtga ccagcctgga agaactgacc
     1021 aaggcgatcg acgagggaaa gctcgacggc atcaaaggcg tcggtgaaaa aaagatcatg
     1081 gcgatcaagc aaggcattca actgcgatcg caggcggtgg ggcgaatggg catcatcgac
     1141 gcgatgccga tcggtgaatc gctcgtcgcg cgcctgcgcg agctgaagca cgtgaaggcg
     1201 gtggaagtcg ccggcagcct gcggcgacgg cgcgagaccg ttggtgatct tgatctcgtc
     1261 tgctcgctca aagatggggt tgacggcacc gccgtcaccg cggcgttcgt cgagtttccc
     1321 gaagtcgagc ggatccttgg ccagggcaac accaaggcca gcgtgctgac cgtcggcgga
     1381 ttgcaggtcg atctgcgcat cgtcccggcg gagaatttcg gcgcggcgct gctctacttc
     1441 accggctcga aagatcacaa cgtgaagctg cgcgggttgg cgctggacaa ggagatgacg
     1501 ctgaacgagt ggggcctgta caagctggcc gagcacgaga aggcggagaa gaaaaccggc
     1561 gaaccgccgc ggtgcaaacc ggtggcgagc aagacggagg cggacgtgta caagaagctc
     1621 ggcatggcat acgtcgagcc ggagatgcgc gaagaccgcg gcgaagtcgc tgcggcgctg
     1681 gcggacaagc tgcccaagct catccaattg tccgacatcc gcggcgatct gcacacgcac
     1741 accaccgcgt ccgacggcac cgccagcatc gccgagatgg ccgaggcggc gatcgcccgc
     1801 gggtacgcgt tcctcggcgt caccgatcat tccaaaagcc aggtcatcgc caatggtttg
     1861 acagccgagc gcctgctcaa gcacgtgaag gagattcaca aggtcaacga ccgctacaag
     1921 ggccaaatcc ggctgttcgc gggttgcgag gtggacattc tcgccgatgg gcgcctggat
     1981 ttcgaagatg cggtgctgaa ggaactggat ttcgtcgtcg ccagtccgca catctcgctg
     2041 aagcaggatg agcgcaaggc gacggaccgg ttgaagcgcg ctctcgacag tcgctatgtg
     2101 aatgtgatcg gccaccccac gggccgattg atcaacagcc gcgagggcct gccggtgaac
     2161 ttcggcgagc tatttcctct ggcggcgaag aacggcaccg cgatggagat caacgccggt
     2221 tacccgcggt tggatttgaa cgagatcaac gcaagggccg ccatcaacgc gggggtgaag
     2281 ctgtcgatcg acaccgatgc gcacggcacg ggcgggtttg acgagatcgt cttcggcatc
     2341 aacgtggcgc gccgtgcctg ggtgacgaag gcggatgtga tcaactgcat gacgctggcg
     2401 gagctggagg cgtttgtggc gcggaagcgg tgagctcgcg caaacggggc ggcagtcgcg
     2461 tgcggcatcg cagaccaagc ggcgttgatt gcgaagccgc aagcggcagc gacgcgcgcg
     2521 gtaaaactaa aagacaaaaa agaacgcgga ccgaagggga gccaccacct ccggtccgcg
     2581 atgaattctt ctactgactg cgctacaaga tatccgatct ttcgaaatcg tcaaccacct
     2641 attgtgcgcg cgacacgcac gcgcgcagac acgcgccgac ttacctcgac aacaactcgc
     2701 gcgccagcgg cgcgatctgc tcccacattg ccgggtcgcg cgcggcgaac atcaagttcg
     2761 ttctcaacca gtcgatcgga ttgccgatgt cgtgccgtcg cgacttcagc acgacgccgt
     2821 agatcggctc gcgcgccagc agcgcgcgca gcgcatcggt cagctgaatc tcgccgccct
     2881 tgcccggtga tgttgacgca agacattgga agatcgccgg cgtgagcacg tatcgcgcgg
     2941 cgatcgcaaa gcggcttggc gcattttccc gtgatggttt ttccacgagc gtatcgagct
     3001 tcatcacgcc gtcgcgcagc atggcgccgc cgacgatgcc gtagcggctc acgcgttcca
     3061 gcggcacttc ttccagtccg ataacggacg atccgaattc gcgatatgcc gccgctagct
     3121 gggcggtcgg cccttcgtcg ccgccgctga agatcgtatc gccgagcagg cagatgaacg
     3181 gctcgtcccc cacatgcttc cgcgcctgcg cgaccgcgtc gcccaggccg cgctgttccg
     3241 gctgccgcac ggaatggatc ttcacgcgtg ccgccagctc gttcaccgat gccagcagcg
     3301 attctttccc acccgctttc agccgctgct ccagctccgc gttgcgatcg aaatgatctt
     3361 cgatcgcccg cttgtcgcgc gacgtgacca gcagcacatc ctcgatcccg cccgccgccg
     3421 cttcttcgat gacgtattgg atcgtcggcc gatcgaggat cggcagcagt tccttcggca
     3481 ccgccttcgc ggccggcagc atccgcgtcc caaatcccgc cgcgggaatg acggctttgc
     3541 gcagtggtct atgtgtcacg ggagcgaatg ttatgtcgag aggggatgcg cgcgaagcat
     3601 cggccggtcc cctcgcccgg tactccggga gagggttagg gtgagggtga ttctgctttc
     3661 cgagagtcac tcgacgttcg aatctaccct caccccagcc ctgtcccggc gtaccgctga
     3721 cgaaccgaca tttttgcagg aatggttggg aaccgaccac gccgggttcg gaggtactcc
     3781 gacgaagcga gattcttctg ctggacgcgc gggaacaatc cgggtcgtcg gagcacctcc
     3841 gaacccggcg tgggtcagcc ccttgctaaa tctgcaaaag tgtcgggccc ttggcggcgt
     3901 accgggcgag ggagtaagac agaaccccgt gcgtgcggcg ctaaggtcaa aagccgcttc
     3961 agcggttggc acgtacgttc aacgtcattc aaaatttcat tcagggtttc gaccttcaca
     4021 gttcggactt gaacccaact actgctctcc cgtcgcaccc ggcatcgtgt ccgcggtttg
     4081 caggcccttg gccaattccg gaagaatccc cttgctgatg tagataggcc tgacgatcgc
     4141 gcgcatcgcg ggcagcttcg atgcgtagct gacggccgcg atcacgcaga tgacgcccgc
     4201 gatcaggatc gttcgcgagg cgccgcgaaa tgcgtcgcca ccgatcgcgg gagtgaggtg
     4261 ataggcggcg taaccggtca gcaggctgcc gaacggcgcc atgccgacga acgccatcgt
     4321 gaagaagctc atcacgcggc cgcgcttgtc gtcctcagcc agcgtttgaa ggatcgtgtt
     4381 cgccgaggcg aagcaggtga tcatgcccca gccggcgatg ggaatgatca gcagcgacag
     4441 ccagagcaga tttgacgaag cgaaagcaat cgcgcccaac ccgaatacca gtgcggcgat
     4501 ggcgatgagc cggccaagac cgacgaccgt ctttcggctg gcgagataga tcgcgccggc
     4561 gagcgcgcca agaccggaca tggtgccgag aaagccgaac acctgcgcgc cgttgcgcga
     4621 tgcaccgccg gtgctgaagt ggtcggcgaa gatcggcatg agggtggaga tcgcgggcat
     4681 gccggtgagg ctgagcatcg ccatcagcag cagcagcacg cggatcggcc ggaagtgcca
     4741 gacgtatttc gcgccctcgg ccaattcgtg aagcacgctc ttgggcgcgc gcggcgggcg
     4801 cggggtcacg cgcatcgcta cgagcgcaat gatcacgccg atgtagctga tcgcgtcgat
     4861 caggaagcac aggctttcac cgacggccgc gatgatcaac cccgccgccg cgggaccgat
     4921 caggcgggcg ccgtgcacca tcgttgaatt gagcgcgatg gcgttcgcca aatcggcacg
     4981 atcggtcacc atttctacga gaaatgcctg gcgggccggc atgtcgaagg cgttgacgag
     5041 cccctggatg atcgccagcc cgatcaagct tggcaccgcg acctgtgtgt tgatgtgccc
     5101 cggccggccg aagtaaaacg cgacggcggc aaggccgaag gactgcagca tcgacagcgc
     5161 ctgcgtgagc acgatgaacc gctgacggtt gacgcgatca gcccacacgc cggcgaacgg
     5221 cgcgagcagg aacatcggca actgcccggc gaaaccgacg atgccgagca ggcgcgtgtc
     5281 tttggtcagg cgatagacga accagacggt ggcgatttgc gtgaggaacg tgccgaccag
     5341 tgagacgagc tgcccgccga agaagaggcg gtaatttcga tggcgaaggg cgcgaaaaat
     5401 cccggcggcc gcgccgccgg tcgtggggga ggaagatggt gcagccaacg gcgcgggttc
     5461 ggccatggtc ggagcgattc tatcccgcgc gccgcggccc gtcccacggc agtaatttgc
     5521 agatcgcgcc acgccgggtt cggaggtacg ccgacgaccc ggatgcttct tccaagatgc
     5581 gccgcaaaat ccgggtcgtc gcgagtacgc tccgaacccg gcgtggttgc tacaacaagg
     5641 ttcttacggc accacggtga cggtgccgaa ctggcggaag tgctgatcga acgcaaacgc
     5701 ggtggtgatg ccgaggcgtt ccatcaccac tttgctggtg cagtcggtga agctccagtc
     5761 tttgtcgaga tagcgttcaa acacatgcca agcatccctg atgtcttgcg gtgtgaggta
     5821 ctcaatatta atcaaaccgg cgagaaaatc cgtcgccggc gttatgcgcc gcgtaacgat
     5881 ggccacgggc gcgcagaagc gttagcgtct cgtcgatgac atagtccgtc gttaccagtc
     5941 gctgccgatt ctgcgcgaac catcgcttgg cctcggcatg tcgatttgcc tctggaaccc
     6001 agagctcgta ccaggcaccg gtgtccacaa agatcaagaa gggtcctcgc cgtagagata
     6061 tttgtcgtga ctggtcccgc cgtcatcggg gatacctttt aggcgggccg acgcttggac
     6121 gcgatcaatc cattcggtga gtgtcttcaa ctcctcagcc gacatttcgg cggcgtcgcg
     6181 atcgacttcc acaaccgctt tggttccttc cggtaacgca acgggttcgg tggggcggaa
     6241 gactccctta tcgaaaatga ctccgatctt cgtcattgct cgctccaatt ctccgtacgc
     6301 gctcccgctc ggacaccaca ttctagtgcc ccaccaagta taccttcaca tacgaataaa
     6361 ccagcgaaaa cgcaccgacg atgacgctgg cccagagcgt gatcttcagg atgcgaatgc
     6421 gattggcgat ttccttgtct cgccactcgg ccaatcggcg gtcgatctcg tcgagcttgc
     6481 ggtcgatgcg cgattcgatc tcgtcgagct tttggttgag ataactgtcg gcggttttct
     6541 tgatgcggtt cgtcaggccg ccgacaaggc cggattcttc gatctcttct ttcaagctgc
     6601 tcggccgcgg tggtggtggg gcggtcggac cggcggtggg gccgctcgcc ggcggtggga
     6661 ggaattggta ctcgcgctcg gcctcgtctt cgccgaccat cttcgacacg agcgaaccca
     6721 gcgcagcgcg gcgcttgcga ttgatcttcg agaagaaccc ttcctgccgg cggagcttgc
     6781 ggatcagtgc gggcaccgaa tcattttcgc ggatcgcgac gccgcggagc atcgccagca
     6841 cgatcagccc gcggtgggac aggccggaga atttcatcga gcggatgcgg gcgatctcct
     6901 gcgcgatctg ttccttgctc gcgttcaccg tgacaggccg acggccccag cggacgacgt
     6961 cgatcatcgc atcgcgatcc agtcccgcga tcagctgccg ccgctcgtgc agcgccgcga
     7021 ccaggtgctg gcgcgtcttg tagcgcgtcg ggtcgagccc gaaatcctgg gcgagcgatt
     7081 cgagttcgtc cttcggcagt tccgacagtt gccgagatgg cgcgctggtc gaagcgatcg
     7141 ccgttgaaac aaccgagggc gtcggcgcgg gcgcgggtgc gacgaccacc ggcggcggtg
     7201 gcggcggcgg tggcgatgtc gccgcgtccg gcagcatctt cacatcggtg ctattgggtg
     7261 atccggctga catgtcgggg ctgcacgttg tggctcaggc tttccctcac cgacttttca
     7321 tccgcggcaa atcgataaat gatcgtgtgc gcgtgccaca ggatcaggaa cgcgacgcac
     7381 agcagcacgc tgatgatcac gtcgccccag aacgcccgga agcgcaaccg cagcccgtgc
     7441 acgatgacgt atcggcggac cagcgagatg tacttatcgc tgccggccag ccagacatac
     7501 cagccccaca acgactgcag cgtgatcacc gtggcataga tcgtcacgac ccagccgagg
     7561 gcgtagcgga aaaag
//
LOCUS       DATIMH010000440         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2122996, whole genome shotgun sequence.
ACCESSION   DATIMH010000440 DATIMH010000000
VERSION     DATIMH010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2122996"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VLI90_07560"
     CDS             <1..617
                     /locus_tag="VLI90_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV14099.1"
                     /translation="ADSSTTRKHSGLGLGLAIVRHLVEAHGGTVVASSAGEWQGSTFT
                     VCLPIKSTVVEDDGSGPSPRGVNDSFPTDGQPELAGLRVLVVDDDLDARLLVSKVLRD
                     CRAEVTAVSSAAEALESLRHHTPHVLVSDIGMPEQDGYALIRSVRGMEQACGSAVMLP
                     AVALTAFARSEDRARALAAGFQQFIPKPVEPAELATIIAMLAKR"
     gene            complement(832..2481)
                     /locus_tag="VLI90_07565"
     CDS             complement(832..2481)
                     /locus_tag="VLI90_07565"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSV14100.1"
                     /translation="MIETLCSCGSAIAAPEAAAGRAVRCGACNHIIRLAAAEAVTPET
                     ALGDFDARLVITGGPVAPLLGSNVGDVIALAGVPDLTLGKSEQAHVRLDGTAVSRLHA
                     KLVRIDFGPSRWKLVDTQSRNGVFVNGHQITERELHDGDIAQIGEYKLKFTVGFPGEA
                     PAPIAGPDVVCSSCGATYAPGTVICTTCGINIKSGRPLVTSKDLADDEVTTERTDKWI
                     RIASYFCPPFLSFMIIPVASEAFATHKPRAIWWITGITVVSSVLFFLCIIASGGMTPN
                     LRNLMLWVGSPEAEHARIEARAKELEKQHPEFFQHRQINNKFEYQVSDRRLQEAVAQA
                     ESRTEERHGEFQWYQLFTHALLHGGLFHLLGNLVFLIVFGLRVNEVIGDLKMAIIYPI
                     LAVLAGGVYALSMRNEPIGAMLGASGAIMGLAGMYFIFFPVQRVHLAVWLRIWVPIVV
                     PLTLFFGYKIFRVRGFWLLLLWILINDVLPTVRASQGDHVAHWAHLGGFMSGMAIALI
                     LLLTRQIHAHGGDILSVTLGSRAWALIGTPEDRAAPPAAVT"
     gene            2801..>3338
                     /locus_tag="VLI90_07570"
     CDS             2801..>3338
                     /locus_tag="VLI90_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSV14101.1"
                     /translation="MDAPSADPRQLRRSLAFIRRVNALLGYTRATLRHLARFSRGWSQ
                     GQRITILDVATGSGDVPAAILRWAKRRGFDVGIVGVDLHEETLRLAAQSAVAGDFRLI
                     RADAMRLPFGDGAFDYAISSMFLHHLDDDAAVAVLREMNRVARRGVIAADLLRRRRAY
                     AWISFFTLLANPMVRHDAR"
BASE COUNT          583 a         1026 c         1154 g          575 t
ORIGIN      
        1 aggcggattc atcgacgacg cggaaacatt cgggcctcgg gctggggctg gcgattgtgc
       61 ggcacctggt cgaagcccac ggcggcacgg tggtcgccag cagcgccggc gagtggcagg
      121 gctcgacgtt cacagtctgc ctgccgatca aatcgacggt ggtcgaggac gatggaagcg
      181 gcccatcacc gcggggggtg aacgacagct tcccgaccga tggacagccg gagctggcgg
      241 ggcttcgcgt cttggttgtc gatgatgacc tggacgcgcg gctactggtg tcgaaggtgc
      301 tgcgcgactg ccgggcggaa gtgacggcgg tatcgtcggc ggccgaggcg ctggagtcgc
      361 tgcggcatca cacgccgcac gtgctggtca gcgacatcgg catgccggag caggatggtt
      421 acgccctgat ccgcagcgtg cgtgggatgg agcaggcgtg cggttcggcg gtgatgctgc
      481 cggctgtggc gctgacggcg ttcgcgcgat cggaagatcg agcccgagcg ctggcggcgg
      541 ggtttcagca gttcattccc aaaccggtgg aaccagcgga gctggcgacg atcatcgcga
      601 tgctggcgaa gcggtgaggt tacttttgtg agaaaagcgc gcggctatgc cgtgggacgg
      661 gagttgagag agcttgtccc acggcagagc cgtgggcttt tgttgcgtga tcagcaccgt
      721 ttgaggattt gagcgaaaaa aaaaaaaagc ccacggctct gccgtgggac ggatgttcga
      781 gcacggtccc acggcagagc cgtgggcttt tttggcgatc tgaaagatcc ttcacgttac
      841 cgccgccggg ggcgcggcgc gatcttccgg cgttccaatc agcgcccacg cgcggctgcc
      901 gagcgtcacc gacaggatgt ccccgccatg cgcgtggatc tgccgcgtga gcagcaggat
      961 cagcgcgatg gccatgccgc tcatgaaccc gccgaggtgc gcccagtggg cgacgtggtc
     1021 cccctgcgag gcgcgaaccg ttggcagcac gtcgttgatg aggatccaca gcagcagcaa
     1081 ccagaatccg cgcacgcgga aaatcttgta gccgaagaac agcgtgagcg gcacgacgat
     1141 cggcacccag atgcgcaacc agaccgccag gtgcacgcgc tgcacgggga agaaaatgaa
     1201 atacatgccg gccaggccca tgatcgcgcc gctggcgcca agcatggcgc cgatgggttc
     1261 attgcgcatc gacagcgcgt agacgccgcc ggccagcacc gccaggatcg ggtagatgat
     1321 tgccatcttc agatcgccga tcacttcgtt cacgcgcaag ccgaagacga tcaggaagac
     1381 caggttgccc aggagatgaa acaatccgcc gtgcagcagg gcgtgcgtga agagctggta
     1441 ccactggaat tcgccgtggc gctcctccgt gcgcgattcg gcctgcgcga ctgcctcttg
     1501 gagccgccgg tcgctgacct ggtattcaaa tttgttgttg atctgccgat gctggaaaaa
     1561 ttccgggtgc tgcttctcca gctccttcgc acgcgcttcg attcgcgcgt gctcggcttc
     1621 ggggctgccc acccacagca tcaagttgcg gaggttcggc gtcatcccgc cgctggcgat
     1681 gatgcagagg aagaacaaca cgctggagac gaccgtgatg ccggtgatcc accagatcgc
     1741 gcgcggctta tgcgtggcaa acgcctccga cgcgactggg atgatcatga acgaaagaaa
     1801 cggcggacag aaatagctgg cgatccgaat ccacttgtcg gtgcgttcgg tggtgacctc
     1861 gtcgtcggcc aggtccttgc tcgtcaccag cggccggccc gatttgatgt tgatgccgca
     1921 ggtcgtgcag atgaccgtgc cgggcgcgta ggtggcgccg cagctgctgc agacgacgtc
     1981 gggaccggcg atcggcgccg gcgcttcgcc ggggaagccg acggtgaatt tcagtttgta
     2041 ttcgccgatc tgcgcgatgt cgccatcgtg caattcgcgc tcggtgatct gatgcccgtt
     2101 gacgaagacg ccattgcggc tttgcgtatc gacgagcttc catcgcgacg ggccgaagtc
     2161 gatgcgaacg agttttgcgt gaagccgcga aacggcggta ccgtcgaggc ggacatgggc
     2221 ctgttccgat ttgccaaggg tcagatccgg gactccggcc agcgcgatga cgtcgccgac
     2281 gttcgacccg agcagcgggg cgaccggccc gccggtgatg acgagccgcg cgtcgaaatc
     2341 ccccagcgcg gtttccggtg tgaccgcctc ggcggcggcg aggcggatga tgtggttgca
     2401 cgccccgcag cgcaccgccc gcccggccgc cgcctcgggg gcggcgatgg cgcttccgca
     2461 ggaacagagg gtttcgatca tccccgtgcc gattgaagag aaaggtgcgc caccgggagc
     2521 gaaacgaccg cccgcggtga cgcgcggatt ttagcacgcg catcggcgcg ccaccagcgc
     2581 ccatcacatc gccccccacg ccgggttcgg aggtactccg acgacccgga tgctttctgg
     2641 agaagaagaa tccgggtcgt cggagtacct ccgaacccgg cgtgggggcg atatgaatga
     2701 tctactgcgc ggccagatgt tgtgacggat tccaagcggc tggtgtaaac tctcgctcaa
     2761 cgtgggatgg ctcgacgttc gacatcgcga ggccgaatgg atggatgctc cgtcggcgga
     2821 cccgcggcag ctgcgccgca gcctcgcgtt catccgccgg gtcaacgcgc tgctgggcta
     2881 cacccgcgca acgctgcggc atctcgcgcg cttcagccgc ggttggtcgc aaggacaacg
     2941 gatcacgatc ctcgacgtcg ccaccggttc cggtgatgtg ccggcggcga tcttgcgctg
     3001 ggcgaaacgc cgcggcttcg acgtcggcat cgtcggcgtc gatctgcacg aagagacctt
     3061 gcgactggcg gcgcaatcag cggtcgccgg cgacttccgt ctcatccgcg ccgacgccat
     3121 gcgactgccg tttggcgatg gcgccttcga ctacgcgatc agcagtatgt tcctgcatca
     3181 tctggacgat gacgccgcgg tggcggtgct gcgcgagatg aaccgcgtcg cccggcgcgg
     3241 cgtgatcgcc gcagatctgc tccgccgccg gcgggcgtat gcgtggatca gttttttcac
     3301 gctgctggcc aacccgatgg tccggcacga tgcccggg
//
LOCUS       DATIMH010000441         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4144884, whole genome shotgun sequence.
ACCESSION   DATIMH010000441 DATIMH010000000
VERSION     DATIMH010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4144884"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VLI90_07575"
     CDS             complement(<1..515)
                     /locus_tag="VLI90_07575"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSV14102.1"
                     /translation="MKPGATDSQPPSDAVVLFDGKNLDEWVSTRDKTPATWTVADGVL
                     VVSKARGAGNIETKRKFRNYQLHAEWKIPENVTGKDQERGNSGIFLASTGPGDAGYEL
                     QILDSYENKTYSNGQAGSIYKQAIPLVNPARKPGEWQTYEVIWMAPVFKADGTVETPA
                     YVTVFFNGVLV"
     gene            complement(699..>2234)
                     /locus_tag="VLI90_07580"
     CDS             complement(699..>2234)
                     /locus_tag="VLI90_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HSV14103.1"
                     /translation="GDIQGIQAELPYLRSLGVTAIYLNPVFRSPSTHKYDTTDYLHVD
                     EAFGKPIDPAEIAGETEDPATWKWTKSDKVLLDFLADAHRQGFKVVLDGVFNHAGGQF
                     GPFLDVKQKGQQSKYADWFNISKWNPVTWISFGGREGGNMPELKKDPVTGLAPGPRDY
                     FLNVTKRWLAPDGDASRGADGFRLDFAQNVPLPFWVTYRKAVKAIKPDAYITGEIWTA
                     ASSYLRGDTWDATMQYQFADALQSFFIGGSKKPITATVFAQRLEHLITINPFQVALNQ
                     MNLLDSHDTDRWASRFVNADRPPPPADPAAGRRGSYNLSKPTDLEWQRMEQSVAVQMT
                     SAGAPMVYYGDEVGMWGASDPDDRQPMIWKDLPPNDDPEVKFNQHLFDTYSRLTAIRR
                     KFATLQTGFTHTVLADDARNLLAFSRDLGDTHVYVLVNRSGETQSVDLPVGPATGNSA
                     MIDWFDPAQTVVKNASASARDGRPQIEPLPGVKPAVVSHNGKATVSLKPWATMILAPA
                     SAN"
BASE COUNT          379 a          625 c          748 g          482 t
ORIGIN      
        1 tgcaccagca cgccgttgaa gaacaccgtg acgtaagcgg gagtttccac cgtaccgtcg
       61 gccttgaaga caggcgccat ccagatcact tcgtacgtct gccactcgcc gggtttgcgg
      121 gcggggttga cgagcggaat cgcctgtttg tagatgctgc cggcctgacc gttggaatag
      181 gttttgttct cgtaggaatc gagaatctgc aactcatacc cggcatcgcc aggcccggtg
      241 gatgccagga agattccgct gttgccgcgc tcctgatctt tgccggtcac gttctcgggg
      301 atcttccatt cggcatgaag ctggtagttg cgaaacttgc gcttggtttc gatgttgccg
      361 gcgcctctcg ccttgctgac caccaacact ccatcggcga cagtccaggt ggccggcgtc
      421 ttgtcgcgag tcgagaccca ctcgtcgagg ttcttgccat cgaagaggac gacagcgtcg
      481 gacggcggct gagaatcggt cgcgccgggt ttcacgactt tgggctccgg ctcccagact
      541 tcggtgtctt tgggattgcc ccgagttcct tttgggggcg ccgccggcgg ctgcgcgaac
      601 agcgaagtgg aagccgccgc gagaagcatt gcgagtgcga atcgagtcat cataacggtt
      661 atcttttagc gtagcgcgcc ggttgtcaat gtgcgggttc aattggcgct ggccggcgcg
      721 aggatcatgg ttgcccacgg cttgagcgag acggtagcct tcccgttgtg cgacaccacc
      781 gccggcttta ctccgggcag tggctcgatt tgcgggcggc cgtcgcgcgc gcttgcggag
      841 gcgttcttga cgaccgtttg cgcggggtcg aaccaatcga tcatggcgga gttaccggtc
      901 gcgggaccga ccggcagatc gacggattgc gtttcgccgc ttcgattcac cagaacgtag
      961 acatgcgtgt cgcccaagtc gcggctgaat gcgagaaggt tgcgcgcatc gtcggccagg
     1021 acggtatgcg tgaagccagt ctgcagggtg gcgaacttgc ggcggatggc agtgagccgc
     1081 gagtaggtat cgaacagatg ctgattgaac ttcacttcgg gatcgtcgtt aggcggcaga
     1141 tctttccaga tcatgggctg gcgatcgtcg ggatcgcttg cgccccacat gcccacctcg
     1201 tcgccgtagt agaccatggg agcgccggcg gatgtcatct gcacggcaac ggattgctcc
     1261 attcgttgcc actccaaatc ggtgggcttg gacaggttgt agctgccccg gcggccggcg
     1321 gcgggatcgg cagggggcgg cggccggtcg gcattgacga agcgtgacgc ccaacggtcg
     1381 gtgtcgtggc tgtccagcag gttcatctga ttgagcgcga cctggaaggg attgatggtg
     1441 atgaggtgct ccaggcgctg cgcgaaaaca gtcgcagtga tgggtttctt cgagccgccg
     1501 atgaagaatg attgcagcgc gtcggcgaac tggtactgca tggtcgcgtc ccacgtgtcg
     1561 cccctcaagt agcttgaagc ggcggtccag atttcacccg tgatatacgc gtcgggcttg
     1621 atggccttta cggctttccg atacgtcacc caaaacggga gcgggacatt ctgcgcgaaa
     1681 tccaggcgga agccatcggc gccgcgcgat gcgtcgccat cgggcgcgag ccatcttttc
     1741 gtgacgttga ggaagtaatc gcggggaccg ggggcgaggc cggtgacggg atccttcttc
     1801 aactccggca tattgccgcc ctcgcggccg ccgaagctga tccatgtgac aggattccat
     1861 ttgctgatgt tgaaccagtc ggcgtacttg gattgctgcc ccttctgctt cacgtcgagg
     1921 aagggaccga attgtccgcc ggcgtgattg aagacgccgt cgaggacgac tttgaagccc
     1981 tggcggtgcg cgtcggccag aaagtcgagc agcactttgt cggacttggt ccatttccag
     2041 gtggctggat cttcggtttc gccggcgatt tccgcggggt cgatcggctt gccgaaggct
     2101 tcgtccacgt gcaggtagtc cgtggtgtcg tacttgtggg tgctcggcga gcggaatacc
     2161 ggattcagat agatggcagt gacgccgagg ctgcgcagat agggaagctc tgcttgtatg
     2221 ccctgtatgt cgcc
//
LOCUS       DATIMH010000442         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2991057, whole genome shotgun sequence.
ACCESSION   DATIMH010000442 DATIMH010000000
VERSION     DATIMH010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2991057"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VLI90_07585"
     CDS             <1..990
                     /locus_tag="VLI90_07585"
                     /inference="COORDINATES: protein motif:HMM:NF007797.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaF family extracellular polysaccharide
                     biosynthesis acetyltransferase"
                     /protein_id="HSV14104.1"
                     /translation="YSTLEAEQVFYTGSTKHEGELQPRIFGNALERRRFLRMRIYPLL
                     PGKWIFRFLYMYVLKEGFMDGLPGLRFCLLIATHEFFIYLKVLELHHLSRSGQLARRD
                     TATAQVIRRASLMPEPPQTPLPPSVPEFPQSEPTERAADAVEAEHPDLSVVERNQSEW
                     TFSEKVKRAIWMFVRATLFRWSFHNWYGWRAALLRFFGAKLAPGVRVRPTASVEIPWH
                     VTIGEGSVVGDYAILYSLGQITIGRGVVVSQYAHLCAGTHDHTTRRFPLLRLPITVGD
                     EAWIAADAFVGPGVKVGDRAVVGARASAFHDVPADQVVGGSPARVIKPRVLRD"
     gene            1101..2225
                     /locus_tag="VLI90_07590"
     CDS             1101..2225
                     /locus_tag="VLI90_07590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV14105.1"
                     /translation="MPSTPSKPLRILHVTAGSDAGGLSRYIFDLSFAMHAAGHQVAVA
                     GQRGAWHWLFETAPFPWIDAPTNGGPFALRKAARLISQYIDKNPIDLLHVHYRRSTLV
                     ARKVQQAHDLPILYTLHLSHILMGFFRRRFSDFGDHTHVASNEARQWLVQSAGVPHEQ
                     ISIIPHGVDLRRFPPANADTTAAAKKTLGLQSTDLVAAYVGRMDAPKNETWMLDLAAE
                     SRQRLPNLKVILVGEGPHEAMLQRRIRRDRLQDRVMLLSQRDPRPIYHAADALLLPSA
                     REGFSLVCAEAMATGIPVLRTRTAGTSELIIENATGRSVDVDHDQFIAAAMDFLSDSV
                     NLKRMGQLAAHHVAEHFTFDRQLHDTVDLYRSLIQLHGGA"
     gene            2288..3283
                     /locus_tag="VLI90_07595"
     CDS             2288..3283
                     /locus_tag="VLI90_07595"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSV14106.1"
                     /translation="MSAAPLTDWTRLSLDHLRGTRVLVTGGAGFIGGHLANALVTLGA
                     HVTVIDDLSGGGDGSSLPPAVKFVRGSITDRRLLDELVAGCRFVFHQAALGSVPRSVE
                     QPQLYYDVNVTGTLNVLEAARKAKVQRVTFAASSSCYGDNPTLPKTESMATSPLSPYA
                     ANKVADEALMQAWAHSYGVDTACLRYFNIFGPRQSPDNAYAAVIAAFAKALVFGKRPT
                     IFGDGEQSRDFTYVDNAVLANLLATRIDKPLLGAPINIACGIRVSVNELAKEMADLLG
                     HPELTPIYKEERAGDVKHSLADLSRANEVLGYKPIVGFREGLASTVSWYKTALKP"
     gene            3554..>3840
                     /locus_tag="VLI90_07600"
     CDS             3554..>3840
                     /locus_tag="VLI90_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14107.1"
                     /translation="MSRSHLCIRTLVLALCISSACVAAESATSEWVHPGPDGKLVYKT
                     TPAGDRVMDFSSAGYMGGGVALPDVPVSVCVQPIGGGKDDTAAIQAAIDEVS"
BASE COUNT          690 a         1281 c         1178 g          691 t
ORIGIN      
        1 tattcgacgc tggaagccga gcaggtcttc tacaccggca gcaccaagca cgaaggcgag
       61 ctgcagccgc gtatcttcgg caacgcgctg gaacgccggc ggttcctgcg catgcgcatc
      121 tacccgctgc tgccgggtaa atggattttt cgcttccttt atatgtacgt gctgaaggaa
      181 ggcttcatgg acggcctgcc gggcctgcgc ttttgcctgc tgatcgcgac gcacgaattt
      241 ttcatctacc tgaaagtttt agaactgcac cacctgagcc ggtccggcca actcgcccgg
      301 cgagacacgg ccaccgcgca ggtgatccgg agggccagcc tcatgcccga accaccccag
      361 acgccgctgc ctccgagcgt gccggaattt cctcaatcgg aaccgaccga acgcgccgcc
      421 gatgccgtcg aagccgaaca cccggacctg agcgtcgtcg agcgcaatca aagcgaatgg
      481 accttcagcg agaaggtaaa gcgcgcgatc tggatgttcg tccgcgcgac gttgttccgc
      541 tggtcattcc acaactggta cggctggcga gcagcactcc tgcgattctt cggcgcgaaa
      601 ctcgcccccg gcgtgcgggt gcggccaacg gcttcggtcg aaattccctg gcacgtgacg
      661 atcggcgaag gctcggtcgt cggcgattac gcgatcctct attcgctcgg ccaaatcacg
      721 atcggccgcg gcgtggtggt cagccaatac gcgcacctgt gcgccggcac gcacgatcac
      781 acgacgcgtc gctttccgct gctgcgattg ccgatcacgg tcggcgacga agcgtggatc
      841 gccgcggatg cgttcgtcgg accgggtgtg aaggtcggag atcgcgccgt cgtcggcgcg
      901 cgagccagcg cgttccacga tgtcccggcg gatcaagttg tcggcggctc ccccgcgcgg
      961 gtgatcaagc cgcgggtgtt gcgagattaa cttcaacgcg taatagccgg gggcggaagc
     1021 cccggtcttt ccgagcgcga aaagaccggg gataccgccc ccggctatca cggccaaagc
     1081 actcatctga aagactcgac ttgccatcga ctccgagcaa accccttcgc atcctccacg
     1141 tcaccgccgg gtccgacgcg ggtgggcttt cgcgctacat cttcgacctg tccttcgcca
     1201 tgcacgccgc ggggcatcag gtggcggtcg cggggcagcg cggggcgtgg cactggttgt
     1261 tcgagacggc gccgttcccg tggatcgatg ccccgaccaa cggcgggccg tttgcgctgc
     1321 gaaaggcggc gcgcctcatc agccagtaca tcgacaaaaa cccgatcgac ctgcttcacg
     1381 tgcattaccg gcgatcgaca ctggtcgcgc gaaaggttca gcaggcgcat gatctgccga
     1441 tcctgtacac gctgcacctg tcgcacatcc tcatgggatt tttccgcagg cggttcagcg
     1501 attttggcga tcacacgcac gtcgcttcca acgaagcccg ccaatggctc gtgcagtcgg
     1561 ccggcgtccc gcacgagcag atctcgatca ttccccacgg ggtggacctg cgccgatttc
     1621 caccggcgaa tgccgacacc accgcggcgg cgaagaagac gctcggcctg caatcgaccg
     1681 acctggtggc cgcgtacgtc ggccggatgg atgcaccgaa gaacgagacg tggatgctgg
     1741 acctggcggc ggaatcgcgc cagcggttgc cgaacctgaa agtgatcctc gtcggcgaag
     1801 gtccgcacga agcgatgctg caacggcgca tccgccgcga ccgtttgcag gaccgcgtga
     1861 tgctgctcag ccagcgcgac ccgcggccga tctatcacgc cgccgatgcg ctgctgttgc
     1921 ccagcgcccg cgaaggcttt tcgctcgtct gcgctgaagc gatggcgacg ggcatccccg
     1981 tccttcgcac gcgcaccgcc ggcacaagcg aattgatcat cgagaacgcg accggccgat
     2041 ccgtcgatgt cgatcatgac caattcatcg cagcggcaat ggatttcttg tccgattccg
     2101 tgaacttgaa gcgcatgggc caactcgccg cccaccatgt tgccgagcac ttcacgttcg
     2161 accggcaact tcacgacacg gtcgacctgt atcggtcgct gattcaactg catggcggcg
     2221 catgaatatg taatcgcgcg cgtgccgccc cttgcatccg cgacgcggtg cgcgagaatc
     2281 cgccgctatg tcggctgccc cactcacgga ttggaccagg ctttccctag accaccttcg
     2341 cggcacgcgc gtgctcgtca ccggcggagc cggtttcatc ggcggacatc tggccaacgc
     2401 gctggtcacg ctcggagcac acgtcaccgt catcgacgat ctcagtggcg gcggagatgg
     2461 ttcgtcgctg ccgccggccg tcaagttcgt gcgcgggtcg atcaccgatc gaaggctgct
     2521 cgatgaactc gtcgccggtt gccggtttgt gtttcaccag gcggcgctgg gttcggtccc
     2581 tcgcagcgtg gaacagccgc agctttatta tgacgtgaac gtcaccggca cgttgaacgt
     2641 gctggaggcg gcccgcaagg cgaaggtgca gcgcgtgacc ttcgccgcca gctccagttg
     2701 ttatggcgac aacccgacgc tgccgaagac cgagtcgatg gcgacgagcc cgctcagccc
     2761 ctacgccgcc aacaaggttg ccgacgaagc gctcatgcag gcgtgggccc acagctacgg
     2821 cgtggacacc gcgtgcctgc gttacttcaa catcttcggc ccccgccaaa gccccgacaa
     2881 cgcctacgcc gccgtcatcg cggccttcgc caaggcgctg gttttcggca agcgcccgac
     2941 gatcttcggc gacggtgaac agtcgcgcga tttcacgtac gtcgacaacg cggtgctggc
     3001 gaacctgctg gcaacgcgca tcgacaagcc gctgctcggc gcccctatca acatcgcctg
     3061 cggcatccgc gtgagcgtga acgaactggc caaggagatg gcggacttgc tcgggcatcc
     3121 ggagctgacg ccgatttata aagaagagcg cgcgggcgac gtgaaacatt cactggcgga
     3181 cttgtcgcgc gcgaatgaag tgctgggcta caagcccatc gtcggcttcc gcgaaggtct
     3241 cgcctcgacc gtcagctggt acaagaccgc gctgaaacca taacgattcg cacgcctcga
     3301 ctgctgcatt tccgcactgc catatccacc aaagcgacac gccgagttcg gaggtactcc
     3361 gacgacccgg attctgccgc gcgtcgagca gaagaatctc gcttcgtcgc gagtacgctc
     3421 cgaacccggc gtgggtcagc tcctcccgat ttggaaaaag cgtcggcgcg tcagcatgta
     3481 catgcggggc tccattgcgc atcgcgccac ggctgacatc aaacactcga gccacttcgc
     3541 ggtattgatg ttcatgtcac gttcgcacct gtgtattcgc accctggttc ttgcgctctg
     3601 catctcctcc gcgtgcgttg ccgctgaatc ggccaccagc gagtgggtgc atcccggccc
     3661 tgatggaaag ctggtctaca aaaccacccc cgcgggtgac cgcgtcatgg atttctcgtc
     3721 cgccggttac atgggcggcg gcgtggcgct gccggacgtg ccggtgtccg tttgcgtgca
     3781 accgatcggc ggcgggaagg acgacaccgc ggccattcag gcggcgatcg acgaggtgtc
//
LOCUS       DATIMH010000443         4754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_104310, whole genome shotgun sequence.
ACCESSION   DATIMH010000443 DATIMH010000000
VERSION     DATIMH010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104310"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VLI90_07605"
     CDS             complement(<1..774)
                     /locus_tag="VLI90_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSV14108.1"
                     /translation="MRQIRPTILFLAPLIALLTMLAPSARAAEPAKADDFFTLVVMPD
                     TQYYTEVQWKTDVYFKGQTQWIVDHRADEHIAFVFQLGDLQQDGNLLRTDDHQYDPLA
                     KDAMAHAKPGEQLPNDFQWRRADEAMKILDDADVPYSAVAGNHDYFHWDRKTLPVNFL
                     KYFGPQRYAGRSWFGGASPANATFPAGMNMYQTFTAGGVKFLSIGLQFAPDARDLAWA
                     QKVVDANPGLPTIVTTHAYLTNKSYDKDRRNIWEELVRKN"
     gene            1381..2424
                     /locus_tag="VLI90_07610"
     CDS             1381..2424
                     /locus_tag="VLI90_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV14109.1"
                     /translation="MKRAFHSHRPVAGFTLVELLVVIGIIAVLIGLLLPALNKAREQA
                     KITVCSSNERQIMQMMQLYATVSKGFLPPFNKGSNGRMNPLTGAYGVPGQSYIESDGL
                     DHWYDSWDEILEEVVNHKPPLSSVTGQTVTNAAMYYCPSDYIPRNKSNGRIPRSYAAN
                     HSKWTWGIDDSKAVNGGPNNATAGVYKAPWSAGAARHLANGTVLTDASPGYDKGEGIR
                     QAKLGEIPSWICIISENWGTSGVYGKPPSTWQAGQSVFAGTDNAVFGQWDNASMDCLI
                     PRFHSTTAKFITDKNSGGNGGNYAFADGHIEFRRWNDMVDIRADYDLRPGNGGNHPPT
                     VYKDPWHWYTLGR"
     gene            2608..3594
                     /locus_tag="VLI90_07615"
     CDS             2608..3594
                     /locus_tag="VLI90_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSV14110.1"
                     /translation="MAIRERENGEASITDVARRARVSVGTVSRVINQHPKVDPALRRR
                     VQIASRQLGFVPRLQHKCIALITGRRNPSLPIGYVSVMTSLVSQYLADSRYAVELIDL
                     ENLELLYEAHTQGAIGVVFDDRLVEAMKIPKLPIMTINAPLVEHGIHSVRADHYQQGV
                     LAAEHCIRRGHRAIGLLCVDVNEWGASERIRGYRATMAAAGHEIDPSWIQSTLTQKPY
                     DTLSRWTSRGVTAILNFSEDTSFEVLHILSNILKLRIGQDISVISLEDMPVYQYLTPP
                     QTTVYQPLAELARLAVKTMFELCDGKANDHQVIDLRLPTQLIERDSVATLSA"
     gene            4057..>4754
                     /locus_tag="VLI90_07620"
     CDS             4057..>4754
                     /locus_tag="VLI90_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSV14111.1"
                     /translation="MAVVLAVLVVIGLSIWTMQSMSTESPDGRETIVFWGGDTVGQDI
                     YAIVNQFEHLPQNLDPKTGQPKYKVVLGNATSPDITGDAQRLLCAIAGEVPPDVVWFD
                     RFAIGEFAGRGALENLLPYIKAQKPNDPYRLNLDDYYQWAVQETSYKRPGSNDEAGIY
                     GVPWEVDMRLLYTNGDLLRQEGFVDPKTKEPLPPRTWEELRKYANALTRYNRPNDKKS
                     GISRLGFAPNFGNS"
BASE COUNT          978 a         1412 c         1444 g          920 t
ORIGIN      
        1 gtttttccgc accagttctt cccaaatgtt tcgccggtcc ttgtcgtagc tcttgttggt
       61 caagtacgcg tgcgtcgtga cgatcgtcgg caacccggga tttgcgtcga ccactttctg
      121 cgcccacgcg agatcgcgcg catcgggcgc aaactgcagt ccgatgctga ggaacttcac
      181 gccgcccgcc gtgaaggttt gatacatgtt catccccgcg gggaacgtgg cgttggcagg
      241 ggaagcgccg ccgaaccagc tgcgaccggc gtagcgctgc gggccgaaat atttgagaaa
      301 attcaccggc agggttttgc ggtcccagtg gaagtagtcg tgattgccgg cgacggcgga
      361 gtagggcaca tccgcatcgt ctaaaatctt catcgcctca tccgcgcggc gccattgaaa
      421 atcgttcggc agctgctcgc ccggcttggc gtgcgccatc gcgtccttgg ccagcgggtc
      481 gtactgatga tcgtcggtgc gcagcaggtt gccgtcctgt tgcaggtcgc ccagctgaaa
      541 gacgaaggcg atgtgctcgt cggcacgatg atcgacgatc cactgcgtct gccccttgaa
      601 gtacacgtcc gtcttccact gcacttcggt gtagtactgc gtgtccggca tgacgacgag
      661 cgtgaagaaa tcatcggcct tcgctggctc cgcagcccgc gctgacgggg ccagcatcgt
      721 caacagtgcg atcaacggcg ccaggaacaa aatggttgga cgaatctgcc tcacggaatg
      781 gcctcctcgc tgaattcaag ccggttaaac agtcggtgct tgaacagcat caactaagcg
      841 acagcatagc gtcttgataa aatatttcac caagatgtga atcattttca gaaggttggg
      901 gcgaaaaacg gccgtccaac gcctgttttt gcggggcgcg tatatgccga aagcactcac
      961 tgccgggcaa aaaaagcata ttcggggcgt gcgatggctt gcgcccaaag ctgccggaaa
     1021 tcaagcttcg tgcacgtttt gcacgcgctc ttaactccag ccaaaccgag cctgaatgaa
     1081 attattcatc gcagatgaaa aagtttcttg acggggacgg gcagcgcggt acgatgtgac
     1141 tgtcgagtgc tgcgaagcgc ttgtcgcggg caccgcactt gaccatgtca tgatggctct
     1201 ttacgcggcg tcgatctaac gggttcgccg tcgcgtggca tatctctcgc gggcgcagca
     1261 ataggcccgg ccatcattca cgggtgccga caacacacgc atcgtggttg agaccacggc
     1321 gcgcctttgt acgagcggtc gcgtggcgaa agcgaggtca gtatgagcag gggcatttcc
     1381 atgaagcggg ccttccactc acatcgtccg gtggccggct tcacgctggt cgaactgctg
     1441 gtggtcatcg gcatcatcgc cgtgttgatc ggtcttctgt tgccggcgct gaacaaggcg
     1501 cgcgagcagg cgaagatcac cgtctgctcg tcgaacgagc ggcagatcat gcagatgatg
     1561 cagctgtacg cgacggtcag caaaggcttc ctgccaccgt tcaacaaggg ctcgaacggg
     1621 cgaatgaatc cgctcaccgg cgcctatggc gtgccggggc agagctacat tgaatcggat
     1681 ggtctcgacc attggtacga cagctgggat gaaattctgg aagaggtggt caaccacaag
     1741 ccaccgcttt cgtcggtcac cggtcaaacg gtgaccaacg ccgcgatgta ttactgcccc
     1801 agcgattaca tcccgcgcaa caagagtaac ggccgcattc cgcgcagcta tgcggcgaat
     1861 cactcgaagt ggacgtgggg catcgacgac agcaaagcgg tcaacggtgg tccgaataac
     1921 gcgacggccg gggtctataa ggcgccttgg tcggccggcg ctgctcggca tctcgccaat
     1981 ggaaccgtcc tcacggatgc cagtcccggc tatgacaagg gcgaaggcat ccgccaagcc
     2041 aaacttggtg agatcccgtc gtggatctgc atcatctccg agaactgggg caccagcggt
     2101 gtgtacggca agccgccgtc tacctggcag gcgggccagt cggtgttcgc ggggacggac
     2161 aacgcggtgt tcggtcagtg ggacaacgcc agcatggact gcttgatccc gcgcttccac
     2221 tcgaccaccg ccaaattcat caccgacaag aacagcggcg gcaacggcgg gaattacgcc
     2281 ttcgcggacg gtcacattga gttccgtcgc tggaacgaca tggttgatat ccgggccgac
     2341 tacgatcttc gcccaggcaa tggtggcaat catccgccga cggtctacaa ggacccgtgg
     2401 cactggtata cgcttggccg gtaatggccc ggcgcatcaa cggctgttga tccaaaacgc
     2461 ccggcagcga atctatgcgg tatataagat agaatcgctg cagggcgtgc ggacaaccac
     2521 gaattggtga aagatttcat cgaatttcca aagtttgatt gacgggaaca tgcccgtcgc
     2581 taagattcga cctgcggact atttacaatg gctattcgag aacgagaaaa cggtgaagct
     2641 tcgatcacgg acgtcgcaag acgtgccagg gtctcggttg gcaccgtctc gcgcgtcatc
     2701 aaccagcacc ccaaggtcga tcccgccctg cgccggcggg tccaaatcgc cagccggcag
     2761 ctcgggttcg tcccccggct tcagcataag tgcatcgcgc tgatcaccgg gcgtcgcaat
     2821 ccttcgctgc ccatcgggta cgtcagcgtc atgacctccc tggtctcgca gtatcttgcc
     2881 gacagtcgtt atgccgtcga gctgatcgac ttggaaaacc tcgagctgct gtacgaagcg
     2941 cacacgcagg gtgcgatcgg cgtggtgttc gacgatcgcc tggtcgaggc gatgaagatc
     3001 cccaagctgc cgatcatgac gatcaacgct ccgctggtcg agcacggcat tcacagcgtg
     3061 cgggccgacc actatcagca gggtgtgctg gccgccgagc attgcatccg ccgcgggcac
     3121 agggcgatcg gcctgctgtg cgtggacgtg aacgagtggg gcgcatccga gcgcatccgc
     3181 gggtatcgcg ccaccatggc cgcggcggga catgaaattg atccgtcgtg gattcagtcg
     3241 acgctgacgc agaagccgta cgacacgcta agccggtgga cctcgcgggg cgtgacggcc
     3301 atcttgaact tcagcgaaga cacatcgttt gaagtcctgc acatcctgag caacatcctg
     3361 aagctgcgga tcgggcagga catttcggtg atctcgctgg aagacatgcc ggtctatcag
     3421 tatctcaccc cgccgcagac cacggtgtat caaccgctgg ccgagctggc gcgcttggcg
     3481 gtgaagacaa tgttcgagct gtgcgacggg aaggcgaacg atcaccaggt gatcgacctg
     3541 cgcctgccga cgcagctgat cgaacgcgac tccgtcgcga cgctgtcggc gtgagtgcgg
     3601 ggccggggtt cggaacttaa accacgaaga cacgaatggc acgaacgggc taaccatgtt
     3661 tgggtggagt ctgcgttggc atgttgttcc aggaatgtgt ttaatgtgcg tcatcctgag
     3721 cggagcacgt acgcgtgcgc agtcgaagga tctggccgag aagccggtga acgtcgagtg
     3781 acaaggaatg gctcggcagc cgcttgcggt ttcgtagatc aagcggcacg gtctgcaaaa
     3841 cgccagcgac tgccgcacga ttcgcgcgag caacagttca tcgtcttcgc ggcaagatcc
     3901 ttcgactccg gcggagtacc gcctgcgctc aggatgacac gctcttcgtg atcttcgtgc
     3961 cttcgtggtt cacgtatttc cttcgtcgtt ccatctgtcg tacccatgcg aacgctcccc
     4021 cagcatcgac gccatcgcgg ttaccttccg ctctggatgg cggtggtgtt ggccgtgctc
     4081 gtcgtcatcg ggctgagcat ttggacaatg cagtcgatgt ccaccgaatc gcccgatggg
     4141 cgcgagacga tcgtgttctg gggcggcgac accgttggcc aggacatcta cgcgatcgtc
     4201 aaccagttcg agcacctgcc gcagaacctt gatcccaaga cgggccaacc gaaatacaaa
     4261 gtcgtcctcg gcaacgcgac ctcgccggac atcaccggcg atgcccagcg gctgctgtgc
     4321 gccatcgccg gtgaagtgcc gcccgatgtc gtctggttcg atcgcttcgc catcggtgaa
     4381 ttcgccggtc gcggcgcgct ggaaaatctg ctgccgtaca tcaaggcgca aaagccgaac
     4441 gatccatatc ggctcaacct cgacgattac taccaatggg ccgttcagga gacgagctac
     4501 aagcggcccg gttccaacga cgaagcggga atctatgggg tcccatggga agtggacatg
     4561 cgtctgctgt acacgaacgg cgacctgctg cggcaggaag ggttcgtcga tcccaagacc
     4621 aaagaaccgc tgcccccgcg cacgtgggaa gagcttcgca agtacgccaa cgcgctgacg
     4681 cgctacaacc gcccgaacga taaaaagtcc ggcatcagcc ggctcggttt cgccccgaac
     4741 ttcggcaact cctg
//
LOCUS       DATIMH010000444         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2412397, whole genome shotgun sequence.
ACCESSION   DATIMH010000444 DATIMH010000000
VERSION     DATIMH010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2412397"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VLI90_07625"
     CDS             <1..293
                     /locus_tag="VLI90_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14112.1"
                     /translation="LYFLSDRPFAAGQMLVAPGYFSGTTDEYRMTRRLKAQGNPMVIE
                     SVEGGAFDGMPSREMRSFAPVFYAYLHEQYEPVNDPAVPDGFRVWRAKGGRR"
     gene            266..1102
                     /locus_tag="VLI90_07630"
     CDS             266..1102
                     /locus_tag="VLI90_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSV14113.1"
                     /translation="MAGEGRPAVSDGDEDHVARAPERTSEQARTEFNAEQFANPYPDG
                     IQHHYWTLARNRLILRTLRSRRMQSESDARAADDVVLDIGCGRGITVEFLRRHGVNAI
                     GADMGTPAPITPDVAPFLHLGQDAMAMPAAFRRQVRTILLLDVLEHIAEPAAFVAALA
                     GAFERCTHLLITVPARQELWSNYDTYYGHFRRYDLRSARSLYPNELFELTSATYAFRL
                     LYAPALVLAAMSRNRSVSISAPTPAMRPVHRLLAAYFGAESAILPRWVPGSSLVLTLR
                     RR"
     gene            1444..2307
                     /locus_tag="VLI90_07635"
     CDS             1444..2307
                     /locus_tag="VLI90_07635"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV14114.1"
                     /translation="MQFRARVFALVIGLAALGVGHNSLAAVIYDSNGFDNVTRFNPAY
                     VAPGEPSVVGNLRDQDPAVNQWLYSGPNTATSTTGTARVINTTSLSPSQSVQVDRISA
                     DNRWAAPNVAITPAGSLNVVDISWDMNVLPTTTDSSKFGPFFGIEAYGGTGVRLGAVG
                     VDATTGELLYESPTFNVVDFNPSTGFGFQPGAGWHHYQMEFNYGSDTYNAWVDGVQKL
                     SGQAFLNASTQFTDADISALQAAPTGFDNATGTAYFDNYVVTSVPEPTSAMLIGLATL
                     ALGCRRRALLA"
     gene            2542..2988
                     /locus_tag="VLI90_07640"
     CDS             2542..2988
                     /locus_tag="VLI90_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14115.1"
                     /translation="MKNKKAVILAAALLCAGSALAAPASKALVVSKATIAAFNPFAVS
                     KHAVKIVAIKTPPKPAAKKDDKKDSKTVTIVTPVKITVPVTTSTPVASPTARPVYTPP
                     PAPAPVVTSPVLTTPVVTLGGGGVRPPYIPPPRSAFLPPAGTGLGP"
BASE COUNT          604 a         1080 c         1092 g          664 t
ORIGIN      
        1 tgctttattt cctgtccgac cgcccgttcg cggcggggca gatgctggtg gcgccgggat
       61 atttttccgg aacgactgat gagtaccgca tgacccgccg gctgaaggcg cagggcaacc
      121 cgatggtcat cgagtcggtc gaaggcgggg cgttcgacgg gatgccgtcg cgcgagatgc
      181 gatcattcgc gcccgtgttt tacgcgtatc tgcacgagca gtatgaaccg gtgaacgacc
      241 cggcggtgcc ggacggcttt cgcgtgtggc gggcgaaggg cggccggcgg tgagcgatgg
      301 agacgaggat catgtcgcgc gtgcgcccga gcggacgtcg gaacaggcgc gcacggagtt
      361 caacgccgag cagtttgcta atccctatcc cgacgggatc cagcatcact attggacgct
      421 ggcgcgaaac cgactgatct tgcgaacgct gcgttcgcgg cggatgcaga gcgaatcgga
      481 tgcgcgcgcg gccgacgatg tggtgctcga catcggctgt ggtcgcggga tcacggtcga
      541 gtttcttcgc cgccatggtg tcaacgcgat cggcgcggac atgggcacgc cggcgccgat
      601 cacgccggac gtcgcgccgt tcctgcacct gggacaggac gcgatggcga tgccggccgc
      661 gtttcgccgg caggtgcgga cgatcctgct gctggacgtg ctcgagcaca tcgccgagcc
      721 cgcggcgttc gtcgcggcgc tcgccggggc gttcgagcgg tgcacccacc tgctgatcac
      781 ggtaccggcg cggcaggaat tgtggtcgaa ctacgacacg tactacggcc acttccgccg
      841 gtatgacttg cgatctgcgc gatcgctgta tccgaacgaa ctgttcgaat tgacgagcgc
      901 gacctacgcc ttccggttgc tgtatgcgcc ggccctggtg ctggcggcga tgtcgcgcaa
      961 ccgatcggtc tcgatctccg ccccgacgcc agccatgcgg ccggtgcatc ggctgctcgc
     1021 cgcgtacttc ggtgccgagt ccgccatcct cccgcggtgg gtgccggggt cgtcgctggt
     1081 gctgacgctg cgacggcggt gacggcaggg ttttcccaga ccaacatccg ggacttcccg
     1141 gagttgcgaa acccctaact ccatcttgac gctgacgctt tgccagccct gcgcggcgat
     1201 tgctatcttg ctgcaattct ttgtgccaca ggtgctaacg cccggagacc agcgaagaag
     1261 ccccgccaca aaaatgcgtg aattcatttt gactcagttt gtctcatcct gtactaataa
     1321 tgaacacggt caggcactcg atgttgttgc ctttggaagg cctgctttcc ctgccgcggg
     1381 gatgtgcagt taaggcaatc ggtgcctttg ggccggggtt ttacttctga agagggagag
     1441 ataatgcagt tccgtgcacg cgttttcgcg ttggtgatcg gtctggccgc gcttggcgtt
     1501 ggccacaact cgctggccgc cgtcatttac gatagcaatg ggttcgacaa cgtgacccgg
     1561 ttcaaccccg cctatgtggc gccgggtgaa ccgagcgtgg tcggcaatct gcgcgaccag
     1621 gacccggccg tcaatcagtg gctgtattcc ggcccgaaca ccgccaccag caccacgggc
     1681 acggcccggg tgatcaacac gacctcgctg tcaccgtcgc agtcggtgca ggtggatcgc
     1741 atctccgccg acaaccgctg ggccgccccg aacgtcgcga ttacgcctgc cgggtcgttg
     1801 aacgttgtgg acatttcctg ggacatgaac gttctgccga ccaccacgga ttcgagcaag
     1861 ttcgggccgt tcttcggcat cgaagcctat ggcgggacgg gcgttcgcct cggcgcggtg
     1921 ggcgttgacg cgaccaccgg tgaactcctg tatgaaagcc cgacgttcaa cgtcgtggac
     1981 ttcaacccca gcactgggtt tggcttccaa cccggcgccg gctggcatca ctaccagatg
     2041 gagttcaact acggcagcga cacctacaac gcgtgggtcg atggggtgca gaagctgagc
     2101 ggtcaggcgt tccttaacgc gtctacccag tttaccgatg cggacatctc cgccttgcag
     2161 gcagcaccca ccgggttcga taacgcgacc gggacggcat acttcgataa ctacgttgtc
     2221 accagcgtgc cggagccgac aagcgcgatg ctgatcggat tggccacgct ggcgctgggt
     2281 tgccggcggc gcgctttgct ggcgtaagct atcatcgaat aaagcattta ggttaagcgc
     2341 acgaagaaca gaggttggca gctgttggtg tctggccacc ggctgtcaac ctgcgttcta
     2401 cgcctcttca aaacaatccg ctggctcgat aacggcttcg aacgcagccc ttgctttggc
     2461 cgagttttgc gttgacttga agttgtggct ggttatcatg ctcgctgggt ttgggatcgt
     2521 gggttcggac ggggaatgca catgaagaac aaaaaggcag tcattctggc tgcggcactg
     2581 ctctgtgcgg gcagcgcgtt ggccgcgccg gccagcaagg cgctggtcgt cagcaaggcc
     2641 acaatcgcgg cgttcaatcc tttcgcggtg tccaagcacg ccgtgaagat cgtggcgatc
     2701 aagacgccgc cgaagccggc cgcaaaaaag gacgacaaga aggacagcaa gaccgtgacg
     2761 atcgtgacgc cggtgaagat caccgtgccg gtgacgacgt caacgccggt ggcttcgccc
     2821 accgctcgac ccgtttatac gccgccgccc gcgccggccc cggtggtgac gtcgcccgtg
     2881 ttgacgactc cagtcgtcac gctcggcggg ggtggtgttc gtccgccgta catcccgccg
     2941 ccgcgtagcg cgttcctgcc gccggcaggg accggtctgg gtccctgagc gatttaaaga
     3001 aagacgattg gccttacaca gggccgatca ccgcagttcc gatggaaccg cgatgatcgg
     3061 ccctttcgcg ttgatggtcc acgttagcgg ccgcgtctac ggggcgtgtg ttcctcacta
     3121 agacgcagcg ctctggcgaa aacaaacggg cggcgtaaac gcgaccgcta aacgcgagga
     3181 cccttgcgtc aaccacgcgt tcaacggtcc atgaatcgtt tccgcgatca gtcgtcgtca
     3241 acgctcgcca cacccctata atctcgcccg tgtccgatgt accgcccagc accaacatcc
     3301 acggccgcgc caccgtcgat aacccgcgca accgcttcgt cccgttgacc gtggtgcgcg
     3361 atgaagacgc ctggatcgat cccgacggcc agccgtcgcc ggccaccgag ttcttcaccg
     3421 acgcgtcgcg ctcgatcatc
//
LOCUS       DATIMH010000445         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_104666, whole genome shotgun sequence.
ACCESSION   DATIMH010000445 DATIMH010000000
VERSION     DATIMH010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104666"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VLI90_07645"
     CDS             <1..791
                     /locus_tag="VLI90_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSV14116.1"
                     /translation="GIVEAHAVCFAAGAATSGYKPVCAIYSTFLQRAYDQIVHDVCIQ
                     NLPVIFCMDRAGFVGDDGPTHMGLYDIAYLRTLPNMTLMAPRNEAELQPMLEHALRLN
                     GPVGIRYPRASTSGKHPDALAPIVQGRAEVLRRGSGVALLAYGNTVDAALDAYDLCAG
                     TKPTVVNMRFAKPLDEALLMELPDTHSHVITLEEHSLAGGFGSAVVEFVSDHGLGLAV
                     ERIGVPNNALVQHDSQPKQRALFGLTGEHVAARVASVLVAPVRP"
     gene            831..1214
                     /gene="secG"
                     /locus_tag="VLI90_07650"
     CDS             831..1214
                     /gene="secG"
                     /locus_tag="VLI90_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSV14117.1"
                     /translation="MLLAAVAPAAKATAAAAAPIKVPVNAVPTNGPLQVNPGLNYSYQ
                     TWFQQHAGWLTHTLGALFVVAALALIVLLALQTTKQEGLSGTIGGRVESAYGRPGAEE
                     QLKRVTGVAAVAFVVIGTILSLTGI"
     gene            1224..>2155
                     /locus_tag="VLI90_07655"
     CDS             1224..>2155
                     /locus_tag="VLI90_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV14118.1"
                     /translation="MPLLDVKNLRTTFRTEDGLVTAVNGLSYSLEAGSTLGIVGESGS
                     GKSVNALSIMRLIQRPGEVHPSSQIFFNGEDLLKKSESDMRKIRGHRIAMIFQDPMTS
                     LNPVLTVGEQISEAVQLHLGYNKKDALEKTIEMFKKVRIPAPEKRISEYPHQFSGGMR
                     QRVMIAMALSCDPQLLIADEPTTALDVTIQAQILELMNEMQRDTGAAIVMITHDLGVV
                     AEVCQNVLVMYGGNMVEYGTAEQIFDNPKMPYTQGLLSSLPRMDIRERHRLEPIPGQP
                     PNLLRLPPGCAFAPRCRYRMPICDEPVPVYDFGAG"
BASE COUNT          407 a          694 c          649 g          405 t
ORIGIN      
        1 tcggcatcgt cgaggcgcac gccgtttgtt tcgccgccgg cgccgcgacc agcggataca
       61 aacccgtgtg cgcgatttac tcgacgttct tgcaacgcgc gtacgatcag atcgtgcacg
      121 acgtgtgcat ccagaatctg ccggtgatct tctgcatgga tcgcgccggg ttcgtcgggg
      181 acgacggccc gacgcacatg ggtctgtacg atatcgcgta tctgcgcacg ctgcccaaca
      241 tgacgctcat ggcgcctcgt aatgaggcgg agctgcagcc gatgcttgag cacgcgctgc
      301 gcctgaacgg gccggtgggc atccgctatc cgcgcgcctc caccagcggc aagcaccccg
      361 atgccctggc acccatcgtc cagggccggg ccgaggtgct gcgccggggc tcaggggtgg
      421 cgcttctggc ctatgggaac accgtggacg ccgccctgga cgcctacgat ctctgtgccg
      481 gaaccaagcc gaccgtggtg aatatgcgct tcgccaagcc gctcgacgag gcgctcctca
      541 tggaactccc ggatacccat tcgcacgtta ttactctaga agaacactcg ctcgcaggcg
      601 gcttcgggtc ggcggtcgta gagttcgtct cggatcacgg tctgggtttg gccgtcgagc
      661 gtattggggt gcccaacaac gccctggtac agcacgactc gcagcccaaa cagcgagccc
      721 tcttcgggtt gacaggcgag catgtggccg cgcgggtagc gagcgttctt gtagcgcccg
      781 tccgtccgtg ataaaactgt acgggtaact aggagtaaca attgatctca atgctgcttg
      841 ccgcggtggc tccggctgcc aaagcgactg ctgcggctgc cgcccctatt aaggtcccgg
      901 tcaacgcggt tcccaccaac ggcccccttc aggttaaccc cggtctgaac tattcgtatc
      961 agacgtggtt ccaacagcat gccggctggc tgacgcacac gctcggcgcg ctgttcgtgg
     1021 ttgccgcgct cgcgctgatt gtgctgctcg cgctacagac caccaagcaa gagggcctct
     1081 ccggaacgat cggcggccgg gtggaatccg catacggacg tccgggcgcc gaagagcaac
     1141 tcaaacgcgt gaccggcgtt gcggccgtcg cgtttgtcgt catcggcacg attctctcgc
     1201 tgaccggcat ctagcgccac cgcatgcccc ttctcgacgt caaaaatctc cgcacgacct
     1261 ttaggaccga agacggcctg gtcaccgcgg tgaacgggct ctcctattcg ctcgaagccg
     1321 gctcgacgct cggcatcgta ggcgaatccg gttcgggaaa atcggtcaac gcgctctcga
     1381 tcatgcgcct catccaacgt ccgggcgagg tgcatccgtc cagccaaatc ttcttcaacg
     1441 gcgaagatct gttgaagaag agcgaatccg acatgcgcaa gatccgcgga catcgcatcg
     1501 cgatgatctt ccaggatccc atgacgtctt tgaatccggt gctaaccgtc ggcgagcaga
     1561 tctccgaggc ggtgcagctg cacctgggct acaacaaaaa agacgcgctc gagaagacga
     1621 tcgagatgtt caaaaaggtg cgcatccccg cacccgaaaa gcgcatcagc gaatatccgc
     1681 accaattctc gggcggcatg cgtcagcgcg tgatgatcgc gatggcgctc tcgtgcgatc
     1741 cgcaactgct gatcgccgac gagccgacca ccgcgctcga cgtcacgatt caagcgcaga
     1801 ttctcgagct gatgaacgaa atgcagcgcg ataccggcgc cgcgatcgtc atgatcacgc
     1861 acgacttggg cgtggtcgcg gaagtttgtc aaaacgtgct ggtgatgtac ggcggcaaca
     1921 tggtcgagta cgggaccgcc gagcagattt tcgacaatcc gaagatgccg tatacgcaag
     1981 gcttgctctc atcgctgccg cgcatggaca ttcgcgagcg gcatcgcctc gaacccattc
     2041 cgggccaacc gccgaacctg ctgcgcttgc cgccaggctg cgcgtttgcg ccgcgttgcc
     2101 gttaccgcat gccgatttgc gatgaacccg tgccggtgta tgactttggc gccgg
//
LOCUS       DATIMH010000446         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_684352, whole genome shotgun sequence.
ACCESSION   DATIMH010000446 DATIMH010000000
VERSION     DATIMH010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684352"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VLI90_07660"
     CDS             complement(<1..1271)
                     /locus_tag="VLI90_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HSV14119.1"
                     /translation="MLGFTETKDRTVPSSAPQARAAPSAPAEAGKTGGVWHAIWSHHQ
                     AVIAILATLGIATNLVLRFGFHAAASTYDIPLWITLGLGGGPLVVGLLAKLVRRQFGS
                     DLLAGISIVTSILLGEYLAGSLVVLMLSGGEALEEIALRSASSVLRALAGRMPSIAHR
                     KNGAQVQDVPLDQVSIGDTVQIFPHEICPVDGVVTDGHGIMDESYLTGEPFHISKTPG
                     SQVLSGSINGESALTIRATKLAVDSRYARIMQVMRASEQASPRIRRLGDQLGALYTPL
                     AVGIALLAWLFSGHASRFLAVLVVATPCPLLIAIPVAIIGAISLAARRGIIIKKPAVL
                     EQVDGCRTIIFDKTGTLTYGEPSLAAILPRPGVKPDQALMMAASLERYSKHPLAVATL
                     QAAEEAKLTPREAARISELPGEGLTGVVDGHT"
     gene            complement(1817..2044)
                     /locus_tag="VLI90_07665"
     CDS             complement(1817..2044)
                     /locus_tag="VLI90_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14120.1"
                     /translation="MSISFEPFASEGHFPTYDPFRGETTRVAIFQLQCRSCGFEPEDS
                     VVAPRLCPKCHSNAWERFARPGSILDNANRY"
     gene            complement(2263..>3295)
                     /locus_tag="VLI90_07670"
     CDS             complement(2263..>3295)
                     /locus_tag="VLI90_07670"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSV14121.1"
                     /translation="RKDKEIDNLRRRLDQRFGLDQIVGQSRLMKDVFAKIQRAAPVDS
                     TVLVLGENGTGKELVAQALHHNSPRKKMPFVAVNCAAVPATLVESELFGHVRGAFTGA
                     TDRRMGRFEQADGGTLFIDEIGDFEIGLQAKLLRVLETLTVTPVGGHEDRKVNVRVLA
                     ATSRDIVKMVKEGTFREDLYYRLNVVTITLPPLRARPDDIPVLVDHFLKEITQNKHTA
                     PRRISPEVMRRLLDYRWPGNVRELRNMLESMLVLSDGDVIVEHDLPERIMEASADVTN
                     PKDLPTGLTMEELEKLAITKALESCSGNRTHAAQKLNISVRTLQRKLRQYELERKIRP
                     MENDVLTEA"
BASE COUNT          580 a         1006 c         1085 g          624 t
ORIGIN      
        1 gtgtgcccgt ccacgacgcc ggtgaggcct tcgccgggca gttcgctgat ccgggcggcc
       61 tcccggggcg tcagctttgc ctcttcggct gcctgaaggg tcgcgacggc cagcgggtgc
      121 ttcgaatacc gttcgagact cgcggccatc atcagggcct ggtcgggctt cacccccggt
      181 cgtggaagga tggcggcgag gctgggttcg ccgtaggtga gggtgccggt cttgtcgaag
      241 atgatggtgc ggcagccgtc gacctgttcg agcacggcgg gcttcttgat gatgatgccg
      301 cgccgggccg cgagcgagat cgcgccgatg atggcgacgg ggatcgcgat cagcagcggg
      361 caaggcgtgg ccacgaccag caccgccagg aatcggctcg cgtgcccgct gaacaaccac
      421 gccagcagtg caatgcccac cgcgagcggc gtgtagagcg cgccgagctg atcgcccagc
      481 cgtcgaatgc gcggcgaggc ctgctcgctg gcccgcatca cctgcatgat gcgcgcgtaa
      541 cgcgaatcca ccgccagctt ggtcgcgcgg atcgtcagcg cgctctcgcc gttgatggac
      601 ccggagagca cttgcgaacc cggcgtcttg gaaatgtgga acggttcgcc cgtgaggtac
      661 gactcgtcca tgatgccgtg cccgtcggtg acgacgccgt cgaccgggca gatttcgtgc
      721 gggaagattt ggaccgtgtc gccgatggag acttgatcca gcggaacatc ctgcacctga
      781 gcgccgttct tgcggtgggc gatcgacggc atgcgaccgg cgagcgcgcg cagcacggac
      841 gatgcgctgc gcaacgcgat ctcttccagc gcttccccgc cggaaagcat caggacgacc
      901 agtgagccgg cgaggtactc gccaagcaag atcgacgtga cgatcgagat cccggcgagc
      961 agatctgaac cgaactggcg gcggacaagt ttggcaagca accccacgac cagaggaccc
     1021 ccgcccagac ccagcgtgat ccacagtggg atgtcatacg tggacgcggc ggcgtggaag
     1081 ccgaagcgca ggacgaggtt ggtcgcgatg ccgagcgtcg cgaggatggc aatgaccgcc
     1141 tgatgatgcg accagatcgc atgccagacg ccgccggtct tcccggcttc ggcgggagcc
     1201 gaaggtgcag ccctggcttg gggcgcagag gaggggaccg ttcggtcctt tgtttctgta
     1261 aatccgagca ttgtccaaat tgttgggcga gcaatcgggt acgggtatcg gggaaaacca
     1321 ctattcgaga tcgcgcgcca gcgcgcgcgt tggcgcgggt tggtcttgca cgttccggtc
     1381 ccctcgcccg gtacgtccga acacgcactt gtcaccgcgc agcgctggcg tactcgccgg
     1441 agtcgaagga ttaagtaagc cgctactaag cttttccgaa tgccccagga acgatgaatt
     1501 tcgcagcgac cgtcgttgca gaaaccgatc ccacgccggg ttcggagggg actcgcgacg
     1561 aagcgagatt tttccgcgcg ccagaggaga agaatccggg tcgtcggagt acctccgaac
     1621 ccggcgtggg gcgatggcga tgttcacttg cccttcgcgt ctgtcttcac gccttcgcgg
     1681 tcgcattgac ttgtctggct taccaccaaa cgcgacacgc ggcggccgcg ctgttgcggg
     1741 gccgccgcgt gtctttgggg gaacataatc ggagcgatac cgctccagac gcttgtccgc
     1801 gctgttgtct gctagcctag tagcgattgg cgttgtcgag gatgctgccc gggcgggcga
     1861 agcgttccca ggcgttgctg tggcacttgg ggcacagccg aggggcgaca acgctgtcct
     1921 cgggttcgaa cccgcaggat cggcattgaa gttggaaaat cgccacgcgg gtggtctcgc
     1981 cgcggaacgg gtcgtacgtc gggaaatggc cttcggaggc gaacggttcg aagctgatag
     2041 acatagttaa cgctccttct aagcttcact tagatgcgat atgcatccca aagccgaagg
     2101 ggaaagagca acagctatgc catgaccggc ggtcgtacga aaaccctcac ggtagaccct
     2161 gcctcacgcg gtggaagcgg aagttatgaa aacgaaagca cccaccaatt tgcttggcgg
     2221 gtgcgtcatg ttgacagttt gtcgcggcgt cggcctgagc tgtcatgctt cggtcaaaac
     2281 gtcgttttcc atcggacgaa tctttcgttc gagctcgtac tgcctcaact tacgttgcag
     2341 cgtgcggacg gagatgttca gcttctgagc cgcgtgcgtg cggttgccgc tgcaggattc
     2401 gagcgccttg gtgatggcca gcttttccag ctcttccatc gtcaggcccg tgggcaggtc
     2461 cttggggttg gtgacgtcgg ccgacgcttc catgatgcgc tctggcaggt cgtgttcaac
     2521 gatcacgtcg ccatcggaca gcacgagcat gctttcgagc atgttgcgca gctcgcgcac
     2581 gttgccgggc cagcggtagt cgagcaaccg ccgcatcacc tcaggcgaaa tccgccgcgg
     2641 ggcggtgtgc ttgttctgcg tgatctcttt caggaagtga tcgaccagca ccggaatgtc
     2701 atccggccgg gcgcgcagcg ggggcagcgt gatcgtcacg acgttcaggc ggtaatacag
     2761 atcctcgcgg aacgtgcctt ccttgaccat cttcacgatg tcgcggctcg tcgccgcgag
     2821 cacgcgcacg ttcaccttgc gatcttcgtg accaccgacg ggcgtgacgg tcaacgtctc
     2881 gagcacgcgc aatagtttgg cttgcaggcc gatttcgaag tcgccgattt cgtcgatgaa
     2941 cagcgttcca ccgtcagctt gttcgaagcg gcccatgcgg cgatcggttg cgccggtgaa
     3001 cgcgccgcgc acgtggccga acaattcgct ctccacaagc gtggcgggga ccgcggcgca
     3061 attcaccgcg acgaaaggca tcttcttccg cgggctgttg tggtgcagcg cctgggcgac
     3121 cagttccttg cccgtcccgt tttcacccag gacgagtacg gtggaatcca ccggcgccgc
     3181 ccgctgaatc ttcgcgaaca cgtccttcat cagccgcgac tgcccgacga tttgatccag
     3241 gccgaagcgc tgatcgagcc gacggcgcaa gttgtcgatc tctttgtcct tgcgc
//
LOCUS       DATIMH010000447         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3569154, whole genome shotgun sequence.
ACCESSION   DATIMH010000447 DATIMH010000000
VERSION     DATIMH010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3569154"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..541)
                     /locus_tag="VLI90_07675"
     CDS             complement(203..541)
                     /locus_tag="VLI90_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSV14122.1"
                     /translation="MRSDKGDMGFDAVVANAGRLNILTALAVDERQEFVQLRQRTHLT
                     DGNLACHAKRLAEAGLIHIDKSFRDGKPVTCLTLTAAGRAALEAHARRVLAAISHRRV
                     AMPVSSNPAG"
     gene            619..1143
                     /locus_tag="VLI90_07680"
     CDS             619..1143
                     /locus_tag="VLI90_07680"
                     /inference="COORDINATES: protein motif:HMM:NF025273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HSV14123.1"
                     /translation="MRVALIFLLLIGATAASPPTTAPATQASAPINATVCIFIAMDCP
                     ISNSYAPEINRIIAEYSPQGLSFKIVYCDADATPEAVAKHAKDYGYSCPIAQDPKHEL
                     AARLHATVTPEAVVLSARDDQPLYRGRIDDRYADVGKKRYEATRHDLRDALEAIVHDR
                     AIEHPVTQAVGCEL"
     gene            1251..2552
                     /locus_tag="VLI90_07685"
     CDS             1251..2552
                     /locus_tag="VLI90_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSV14124.1"
                     /translation="MNRSTLVLVFALLITASAARAADLQPTFTRDIAPIVYANCVNCH
                     RPGEVAPFPLISYSDVKKHADLITTVTEKRQMPPWKPEPGFGHFVGERRLTDVQIKAI
                     ADWVTSGMPEGDAKDLPPAPKFTDGWSLGEPDLVVKLDRPFTLPADGDHGRDVYRCFV
                     IPLKFDEDKYVTAVEFRPGNPKIVHHALFFLDASGAAQKKVEANTDGQPGFATFGGPG
                     FVPTGSLGGWAPGATPSPLPEGWARRMRKGSDLVIQEHLHPSGKIETEQSTLGIYFAK
                     RTPDRIAGGAAVRNFKINIPPGDSAYVITAQMTVPVDVDLIGITPHAHLICKDMQGNA
                     TLPDGTKVPLVWIKDWDFNWQGQYRYAEPIRLPAGTVIDMKYTYDNSASNIHNPNTPP
                     KAVHFGEQTTDEMAFLFLQFSPVHRADWLKILKTRASGFGG"
     gene            complement(2608..>2977)
                     /locus_tag="VLI90_07690"
     CDS             complement(2608..>2977)
                     /locus_tag="VLI90_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14125.1"
                     /translation="GLSIFLLPFSICHLMGCEVKQPPPLEPVSHAPLVVDPAMRQRQW
                     PVSVAHFANGQTVAWPTGAIFRHRANEPTWQGAITDTPIFVANSVTMPVVYLFTPPWQ
                     PVVYPSGEIEASYHMMPPMK"
BASE COUNT          592 a          932 c          923 g          530 t
ORIGIN      
        1 accaagtttc tcatgcgcag catcctcgcg tcatatgtct ggaggtttaa cgagaagggg
       61 ggtcagtcta tcgaggaaac gtcgaattgg ctaacgttca acgtccaact ttcgatgctc
      121 aactcgatgc tcaacaacga acaagcgagc cggaccgatc gcttatttaa tgttgaaatg
      181 ttgagcgttg aaagtttgga cattaaccag ctggattgct gctcaccggc atcgccacac
      241 ggcggtggct gatggcggcg aggacccggc gggcgtgcgc ttccaacgcg gcccggccgg
      301 cggcggtcag cgtgaggcac gtgacgggct tgccgtcgcg gaaagatttg tcgatgtgga
      361 tgagccccgc ctcggcaagg cgcttggcgt gacacgccag gttgccgtcg gtcaggtgcg
      421 tgcgctggcg caactgcacg aactcctgcc gctcatcgac cgccagagcc gtcaggatgt
      481 tcaaccgccc cgcgttggcc acgaccgcat caaagcccat gtcgccctta tcggaccgca
      541 cgtgtcagaa actttgctga aaccggaccg ccccggcgat aatatcccgc ggtctcgatc
      601 gttttacctt ttatgcaaat gcgcgtcgcg ctgattttcc tgctgttgat cggcgcgacc
      661 gcggcgtcgc cgccgacgac ggcgccggca acgcaagcat cggcgccgat caacgcgacg
      721 gtgtgcatct tcatcgcgat ggattgtccg atctcgaaca gctacgcgcc agagatcaac
      781 cggatcatcg cggagtattc gccgcaagga ttgtcgttca agatcgtcta ctgcgacgcc
      841 gacgccacgc cggaggcggt ggcgaagcat gcgaaggatt atgggtactc gtgtccgatc
      901 gcgcaggatc cgaagcacga gctagccgct cgcctgcacg cgacggtgac gccggaggcg
      961 gtcgtgctgt cggcgcggga tgatcagccg ctgtaccgcg ggcggattga cgatcgatac
     1021 gccgatgtcg ggaagaagcg ttatgaggcg acgaggcacg atcttcgcga tgcgctggag
     1081 gcaatcgtgc acgatcgggc gatcgagcat ccggtgacgc aggcggtggg gtgtgagttg
     1141 tagtccttag ggaagagtcc tcgagaacag tcccacggca gagccgttgg gacgttcgtg
     1201 acgcgccaaa agcccaccgc tatgccgtgg gaccgatttc ggaaaaacct atgaaccgct
     1261 caacgctcgt ccttgtcttc gcgctactca tcaccgccag cgccgcccgc gccgccgatc
     1321 tccaaccgac cttcacgcgc gacattgcgc cgatcgtcta cgcgaactgc gtcaactgcc
     1381 atcgccccgg cgaagtcgcg ccgtttccgc tgatctctta cagcgacgtg aagaagcacg
     1441 cggacctgat cacaacggtg accgaaaagc gccagatgcc gccgtggaaa ccggaaccag
     1501 gcttcggcca ctttgtcggc gagcgccggc tgaccgacgt gcagatcaag gcgatcgccg
     1561 actgggtgac gagcggcatg cccgaaggcg acgccaagga tctcccgccg gcgccgaagt
     1621 tcaccgatgg ctggtcgctg ggcgagccgg acctggtcgt caagctcgat cgccccttca
     1681 cgctgcccgc ggacggcgac catggccgcg atgtctatcg ctgcttcgtc atcccactga
     1741 agttcgatga ggacaagtac gtgaccgccg tcgaattccg cccgggcaat ccgaagatcg
     1801 tgcaccacgc gctgtttttc ctggatgcca gcggcgcggc gcagaagaaa gtggaagcga
     1861 acaccgacgg tcagcccggc ttcgccacgt tcggcggtcc cgggttcgtc cccaccggca
     1921 gcctgggcgg ctgggcaccg ggcgcgacgc cgtcaccgct gcccgagggc tgggcgcgcc
     1981 gcatgcgcaa ggggtccgat ctggtcatcc aggaacacct gcatcccagc ggcaagatcg
     2041 aaacagaaca atcgacgctc ggcatctact tcgccaagcg cacacccgat cgcatcgccg
     2101 gtggcgccgc ggtgcggaac ttcaagatca acattccgcc cggcgacagc gcgtacgtca
     2161 tcaccgcgca gatgaccgtg ccggtggacg tcgatctaat cggcatcacg ccgcacgcgc
     2221 acctgatctg caaggacatg cagggcaacg cgacgctgcc ggacggcacg aaggtcccgc
     2281 tggtctggat caaggattgg gatttcaact ggcagggcca gtaccgctat gccgagccga
     2341 ttcgcctgcc cgcggggacg gtgatcgaca tgaagtacac gtacgacaat tcggcgagca
     2401 acatccacaa tccgaacacg ccgccgaagg cggtgcattt tggtgaacaa acgacggacg
     2461 agatggcgtt tctgttcctg cagttttcgc cggtgcatcg ggcggattgg ttgaagatcc
     2521 tcaagacgcg cgcgagtgga tttggtggct gaaacaacca agccccggat gtacatccga
     2581 ggcttcgtca gtcatcgata tggttcttca cttcatcggc ggcatcatgt gatacgacgc
     2641 ttcgatctcg ccgctcgggt acaccacggg ctgccacggt ggcgtgaaca ggtacaccac
     2701 cggcatcgtc actgagttgg cgacgaagat cggcgtgtcg gtgatcgcgc cctgccatgt
     2761 cggttcattc gctcgatggc gaaagatcgc ccccgtcggc caggcgaccg tttggccatt
     2821 tgcaaaatgc gcgactgaca ccggccattg ccgctgccgc atcgccgggt ccaccaccag
     2881 cggcgcatgg ctcaccggtt ccaacggcgg cggttgcttc acttcgcagc ccatcaaatg
     2941 gcaaatggaa aatggtaaaa ggaaaatgga cagcccg
//
LOCUS       DATIMH010000448         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2124741, whole genome shotgun sequence.
ACCESSION   DATIMH010000448 DATIMH010000000
VERSION     DATIMH010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124741"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..433)
                     /locus_tag="VLI90_07695"
     CDS             complement(8..433)
                     /locus_tag="VLI90_07695"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HSV14126.1"
                     /translation="MASEPITTFNVVKEVQIAAPPEVVFETVLEPQGPMKDMNMKLEA
                     WVGGRWFRDLGNNTGHLWGHVQVIKPPKVLELIGPTMMSYPAISHIQYRLTEQAGGTL
                     LKFTHRAMGLIEPGLEQNVTKGWGEMLEMIRAAAEKKRG"
     gene            complement(423..821)
                     /locus_tag="VLI90_07700"
     CDS             complement(423..821)
                     /locus_tag="VLI90_07700"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSV14127.1"
                     /translation="MARAATTSDVFNAIAEPRRREIVDLLARESSRDVSEIVLKLGLS
                     QPAVSKHLGVLRKVGLVDVQKAGQRRLYRLNPQELKPVHDWIQTFERFWTDHLGSIKQ
                     VAEQKAKQLAARDRAASHPQPNSKEKRHGQ"
     gene            878..1144
                     /locus_tag="VLI90_07705"
     CDS             878..1144
                     /locus_tag="VLI90_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14128.1"
                     /translation="MNGTSHAGFGGIVFNDTNANDRLDAGERRVVTDRFGRYTFDNLG
                     KATMRVILLLPKGCRGVSPVNDFRLSPRQRLAHNGSNSRKGSRP"
     gene            1141..2064
                     /locus_tag="VLI90_07710"
     CDS             1141..2064
                     /locus_tag="VLI90_07710"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV14129.1"
                     /translation="MKQDGISRRGFLRTSAVVTGAAMLSAASASSLFAAAGDAAGVKR
                     TATDQVQLGKTAIMLSRVGIGTGSNNGQEQTVHGQEYFTKLIHHAFDSGITYIDTAEA
                     YKTFNWIAEATKGLPRERLFIQSKVDGRPDDVLAVIDRHRSVFKTDYIDSLLIHCMTK
                     PGWTDPWKRIMDGFNEAQSRKWIRAKGVSCHTLPALVDGNKSDWPEVHLVRVNPQGKF
                     MDATKDAWGRGAVDVQPVLDEIKSMHDKGRGVIGMKIMGNGSFTDPADREKSVRFAMA
                     NRNIDAIVIGMTSTQQVDENLALVNRALAAA"
     gene            2205..3122
                     /locus_tag="VLI90_07715"
     CDS             2205..3122
                     /locus_tag="VLI90_07715"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSV14130.1"
                     /translation="MRTLTTAFAAILLAAPLCLAQTQPTSRPVAAPGEQVQLVSADFN
                     FTEGPAADAAGNVYFTDQPNDRILKWDAASGKISTFLQPCGRSNGMSFDAAGNLISCA
                     DEHNELWSIAPDGSHIVILKDLGGKLLNGPNDIWIRPTDGAIYLTDPLYKRKWWTRDP
                     AMQQPSRAVIYISPDRKTVRRAAEDFKQPNGIIGTPDGRRLYVSDIDARQTFVYDIAD
                     DGQLTNKRPFCAMGSDGMTIDDQGNVYLSNKGVSVFNPSGSLIDHIDIKQPWTANVCF
                     GGADRQTLFITASKAVYTLRTRVKGVGSP"
BASE COUNT          678 a         1127 c         1029 g          625 t
ORIGIN      
        1 tgtcgaacta cccgcgtttc ttttccgcgg cggcgcggat catctccagc atttcgcccc
       61 agcctttggt gacgttttgt tcgagcccgg gctcgatcag gcccatcgcg cggtgcgtga
      121 acttcagcag cgtgccgccg gcttgttcgg tcaggcgata ctggatgtgc gagatcgccg
      181 gatacgacat catcgtcggg ccgatcagct ccagcacttt gggcggcttg atcacctgca
      241 cgtggcccca gaggtggccg gtgttgttgc ccaggtcgcg gaaccagcgc ccgccgaccc
      301 acgcctccag cttcatgttc atgtctttca tcggaccctg cggctcgagc acggtctcga
      361 acaccacctc cggcggggcg gcaatctgaa cctctttgac gacgttgaac gttgtgatcg
      421 gctcactggc catggcgctt ctcctttgaa ttgggctgtg ggtgagacgc cgcgcgatcg
      481 cgcgcggcaa gttgtttggc tttttgctcg gccacctgct tgatgctgcc caggtggtcg
      541 gtccagaacc gctcgaaggt ttgaatccag tcgtgcacgg gtttgagctc ctgcgggttc
      601 agccgataca accggcgttg ccccgccttt tgaacgtcaa ccaaacccac cttgcgcagc
      661 acccccagat gcttggagac ggccggctgc gacaggccga gcttcagcac gatctcgctg
      721 acgtcgcgcg aactttcgcg agccagcagg tcgacaatct cccgccgccg cggttcggcg
      781 atggcgttga agacgtcgga ggtggttgcc gcgcgggcca tgcgatcatt atattccgta
      841 ttaggcatat atatcaagcg aaaaaccttt tggctggttg aatggcactt cccacgccgg
      901 gttcggcggc atcgtgttca acgacaccaa cgccaacgat cggctggacg cgggcgaacg
      961 ccgcgtcgtc accgatcgct ttggccgcta tacattcgac aatctcggca aggccacgat
     1021 gcgcgtgatt ctgctgctgc cgaagggatg tcgcggcgtc tcgccagtca atgattttcg
     1081 cttgtcgcct cggcaacgct tggcgcacaa tgggagtaat tcaagaaaag gatcacgccc
     1141 atgaagcagg atggcatcag tcgtcgcggg ttccttcgca cgtccgccgt ggtgaccggg
     1201 gcggccatgc tcagcgccgc cagcgcatcg tcgctgttcg ccgccgcggg tgacgcggcc
     1261 ggcgtcaagc gcactgcgac cgatcaagtt caactcggca agaccgccat catgctcagc
     1321 cgcgttggca tcggcaccgg atcgaacaac ggccaagagc aaaccgtcca tggccaggag
     1381 tatttcacga agctgatcca ccacgcgttc gacagcggca tcacctatat cgacaccgcc
     1441 gaggcgtaca agacgtttaa ttggattgcc gaggcgacca agggcctgcc gcgcgagcgg
     1501 ctgttcatcc aatcgaaggt ggacggccgg ccggatgatg tgctggcggt catcgatcgc
     1561 catcgcagcg tcttcaagac cgactacatc gacagcttgc tgatccactg catgaccaag
     1621 ccggggtgga ccgatccgtg gaagcgcatc atggacggct tcaacgaggc gcagtcgcga
     1681 aagtggattc gcgccaaggg cgtgtcgtgt cacacgctgc cggcgctggt ggatgggaac
     1741 aaatccgact ggcccgaggt gcacctggtt cgcgtgaacc cgcaggggaa gttcatggac
     1801 gcgacaaaag acgcctgggg ccgcggggcc gtcgacgtgc agccggtgct ggacgagatc
     1861 aagtcgatgc acgacaaagg ccgcggcgtg atcggcatga agatcatggg caacgggtcg
     1921 ttcacggatc cggcggatcg cgaaaaatcc gtgcggtttg cgatggcgaa tcgcaacatc
     1981 gacgcgatcg tcatcggcat gaccagcacg cagcaggtgg atgagaattt ggcgctggtg
     2041 aaccgggcac tcgccgctgc atagtcttac tccctcgccc acgtactcat gggagagggc
     2101 tggggtgagg gtgatttcga gcgtcgggcg atcagctaat tctgaatcac cctcaccctc
     2161 tcccatgagt acatgggcgc ggggaccaga gcggcggatc taatatgcga accttgacga
     2221 ctgccttcgc ggcgattttg ctcgcggcgc cgctttgttt ggcgcagacg caacccacct
     2281 cccgccccgt cgccgccccc ggcgagcagg tgcagctcgt ctccgccgac ttcaacttca
     2341 ccgaaggccc cgccgccgac gctgccggca acgtttactt caccgatcaa cccaacgatc
     2401 gcatcctgaa atgggacgcc gccagcggga agatcagcac gttcctgcag ccctgcgggc
     2461 gaagcaacgg catgtccttc gacgccgccg gcaacctcat ctcgtgcgcc gatgaacaca
     2521 acgaactgtg gtccatcgcg cccgatggct cgcacatcgt catcctcaag gacttgggcg
     2581 gcaaactgct aaacggcccc aacgacatct ggatccgccc aaccgacggg gcgatctacc
     2641 tcaccgaccc gctttacaag cgcaagtggt ggacgcgcga cccggcgatg cagcagccgt
     2701 cgcgcgcggt gatctacatc tcccccgatc gcaaaaccgt ccgccgggcc gccgaagatt
     2761 tcaagcagcc caacggcatc atcggcacgc ccgatggccg ccggctttac gtctccgaca
     2821 tcgacgcccg gcaaaccttc gtctacgaca tcgccgacga cggccagctg accaacaagc
     2881 gccccttctg cgcgatgggc tccgacggca tgacgatcga cgaccaaggc aacgtctacc
     2941 tcagcaacaa aggcgtcagc gtcttcaacc ccagcggttc gctcatcgac cacatcgaca
     3001 tcaaacaacc ctggaccgcc aacgtctgct tcggcggcgc cgaccgccaa acgctcttca
     3061 tcaccgccag caaggcggtg tacacgctgc gcacgcgcgt caaaggcgtg ggcagcccgt
     3121 gacgtctgcc aagcgcggac gacagaacac gagcggcaca acagagccgc ttgcggcttc
     3181 gcaaacgcag gcgcgcaaga aaaaaattgt cgcgagttgc gcggctgcga cagatacaag
     3241 tctttactgc agaagatcat gtgctgatac atcgccggaa aaagctcgcg tccagttgcg
     3301 ccagatgcgc ttttatatcc aagctgccca gtcgccccag cctgacacgc atcgggggaa
     3361 accgtcgctc ggggcgatcg cttttaccgt tccccacgcc gggatcggag gtactccgac
     3421 gacccggatg cttctcgcgc gcacagcagg atcccggtc
//
LOCUS       DATIMH010000449         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_105762, whole genome shotgun sequence.
ACCESSION   DATIMH010000449 DATIMH010000000
VERSION     DATIMH010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105762"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1645)
                     /locus_tag="VLI90_07720"
     CDS             complement(95..1645)
                     /locus_tag="VLI90_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HSV14131.1"
                     /translation="MSPVDKTIADATIVRRQHSAAIVRRSLDAVKNPRVPFRMPALRP
                     LRFPKRIIDIRDFGAVADGQTPCTVAIARAIQACVEAGGGRVLIPAGSWFTGAIHLRD
                     NIDLHLAEGATVRFSSQPADYLPAVFVRWDGQECHNYSPFIYASACRNVAITGAGSLM
                     GQGAQWDVTKKSQNLALARLHQMVADDVPVEQRRFAVEEFPLRPQMICFINCAGVLLE
                     DFSITEGGPARTIHVAYCQDVLIRRLRVAASARPGNDGISIDSTCNAIVEDCQLQTAD
                     DCVALKSGLNGDGCRVGKATENVVVRRIRATGGLGGIAIGGEIAGGIRNVFVHDCNYA
                     GLAAGISIKAKPGRGGTVENVFVDNVTMGNIPGDAIQMMAEGSSSAKADRKPMTFRNI
                     QMRNITCEQADTAVRMVGLADAPLREVVLENVNITAREGLYCTASNGVRLINVRITPR
                     SGPVLSVKDSQHIVIDGLNSVHGRSVFLDLRGRQIRDVRLKGDQRTRVRPVVVLGVDV
                     PKDAIIHE"
     gene            complement(2042..3244)
                     /locus_tag="VLI90_07725"
     CDS             complement(2042..3244)
                     /locus_tag="VLI90_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HSV14132.1"
                     /translation="MPRTKTSPSESPPRRRMRKKYPNVALLIETTRSYGRNVLRGIND
                     YARIRGPWLFHLPNDIPVRGLPSSDEWSGDGVIAQPRQDQEFLRQLIDSGLPVVSLSG
                     PPGTGGLPAVRANQEAVAELAIAHFRDRGFVRFAYCGTPGEWDWPPTGQNFRRLTEEA
                     GYDCNVYDPLTDPGNRTMRVAHLAHWLRSMKKPVGLLAQNDMRAREVLDACRTVNISV
                     PEEIAVLGVNDDELICEMANPPLSSVIHNARRVGYEAAAMLDRLMSGKSVVADIVVDP
                     LGVNARQSTDLLAIEDSEVAKAVRFIREHACEGIRVDDVLDEVALSRRSLEKRFRQAV
                     GRPPHMEIRRVQLERVKQLLVTSDFKLDKIAQVTGFSTAQYLAGLFHRTVKMTPGAWR
                     QAGRSGAK"
BASE COUNT          571 a         1060 c         1074 g          608 t
ORIGIN      
        1 ccctaaccct ctcccatgag tacatgagcg aggggaccag aacgcggaca atcttccgga
       61 ttacggtcgg gaaagggaag agacgggagc cgcctcattc gtgaatgatg gcgtccttcg
      121 gcacgtccac acccagcacc accaccgggc ggacgcgggt gcgctgatcg cctttgaggc
      181 gcacgtcgcg gatctgccgg ccgcgcaggt caaggaagac gctgcggccg tgcacgctgt
      241 tcaagccgtc gatcacgatg tgctggctgt ccttcaccga cagcaccggt ccgctgcgcg
      301 gcgtgatgcg cacgttgatc aaccgcacgc cgttgctcgc cgtgcagtac aacccttcgc
      361 gcgcggtgat gttcacattc tccagcacca cttcccgcag cggcgcatcg gccaatccca
      421 ccatgcgcac ggcggtgtcg gcctgctcac aggtgatgtt gcgcatttga atgttgcgga
      481 acgtcattgg cttgcgatcg gccttcgcgc tggatgagcc ctcggccatc atctgaatcg
      541 cgtcgccggg gatgttgccc atggtgacat tatcgacgaa gacgttttcg accgtgcccc
      601 cgcggccggg cttggccttg atgctgatgc ccgcggccag gcccgcgtag ttgcagtcgt
      661 gcacgaagac gttgcggatg ccgccggcaa tttcaccgcc gatggcgatg ccgcccagcc
      721 caccggtggc gcggatgcgg cggacgacga cgttctcagt cgctttgccc acgcggcaac
      781 cgtcgccgtt caagccggac ttcagcgcca cgcaatcatc cgccgtctgc agctggcagt
      841 cttcgacgat cgcgttgcat gtcgagtcga tgctgatgcc atcatttccg ggccgggccg
      901 aagccgcgac ccgcaaccgg cgaatcaaca cgtcctggca atacgcgacg tggatggtcc
      961 gcgccggtcc gccttcggtg atgctgaagt cttccagcag gacgcccgcg cagttgatga
     1021 agcagatcat ctgcgggcga agcgggaatt cttcgacggc gaatctgcgc tgctcgaccg
     1081 gcacgtcgtc ggccaccatc tggtgcagcc gagcgagcgc caggttctgc gacttcttgg
     1141 tcacatccca ctgcgcgccc tgtcccataa gcgacccggc gccggtgatg gcgacgttgc
     1201 gacaggcgct ggcgtagatg aacggggaat aattgtggca ctcctgcccg tcccaacgca
     1261 cgaatacggc gggcagataa tcggccggct ggctgctgaa tcggacggtc gcgccttcgg
     1321 ccaggtgaag atcgatgttg tcgcgcaggt gaatcgcgcc ggtgaaccag ctgcccgcgg
     1381 ggatcagcac gcgacccccg ccggcttcga cgcaggcttg gatcgcccgc gcgatggcga
     1441 cggtgcacgg tgtttgtcca tcggcaacgg cgccgaaatc gcggatgtcg atgatgcgct
     1501 tggggaaccg caacggccgc aatgcaggca tgcggaatgg cacgcgcggg ttcttcaccg
     1561 cgtcgagcga tcggcgcact atggccgccg agtgctgccg gcgcacgatc gtcgcgtcgg
     1621 cgatggtctt atcgactgga ctcatacgct gtggttcccg ctcggccgcg cgccgctggt
     1681 cattgcccgc gcacgactga gcccaatgcc cccgccttac acgcatccgc ctgctggtgg
     1741 cacaaatcgc ctcgtacgga cagacgaggc ataggggagc tggtcatcga gtgatgactc
     1801 tatcggtatg accaaaccaa cggagtccag ttttgcgaaa aagttttgtc atccagcgca
     1861 gggaggggga gtgtgtgcgc tgaaggtaga gggtgcgcgc cgacgccggt gtctgaggta
     1921 ctcccaagac ccgggttttt cgcgcggacg tacccgggtc ttcggagtac ctcagccccg
     1981 gcgtcggggg cgcgcctgaa acatcaggag caactgcgcg cgggacggga aatgcgctgc
     2041 cttatttcgc gccggaccga cccgcttggc gccacgcgcc cggggtcatc ttcaccgtgc
     2101 gatggaacag cccggcaaga tattgcgcgg tcgaaaaacc cgtcacctgc gcgatcttat
     2161 cgagcttgaa atcgctggtc accagcagct gtttgacccg ctccaattgc acgcggcgga
     2221 tctccatgtg cgggggccgc cccaccgctt gccgaaagcg cttttccagc gaccggcgcg
     2281 acaacgccac ctcgtccagc acatcgtcca cccggatgcc ttcgcaggcg tgttcgcgaa
     2341 tgaagcgcac ggctttggcg acttcggaat cttcgatcgc cagcaggtcg gtcgattgcc
     2401 tcgcgttcac gcccagcgga tcgacgacga tgtcggcaac gaccgatttt cctgacatca
     2461 accggtccag catggccgcc gcttcgtagc ccacccggcg ggcgttgtgg atgacgctgg
     2521 acaacggcgg gttggccatc tcgcagatca gctcatcgtc gttcacaccc agcacggcga
     2581 tctcttccgg cacggaaatg ttgacggtgc ggcaggcgtc gagcacctcg cgcgcccgca
     2641 tgtcgttctg cgcgagcagg cccaccggct tcttcatcga tctgagccaa tgcgccaggt
     2701 gggcgacgcg catcgtgcgg ttgccgggat cggtcagcgg gtcgtacacg ttgcagtcgt
     2761 aaccggcttc ttcggtcaac cgccggaagt tttgaccggt cggtggccag tcccattcac
     2821 cgggcgtgcc gcagtatgcg aagcggacga acccgcggtc gcggaagtgg gcgatagcca
     2881 gctcggccac cgcttcctgg ttggcgcgca cggcgggcaa accaccggtg ccgggtgggc
     2941 cggatagcga gacaacgggc aagccgctgt cgatcaattg gcgaaggaat tcctggtcct
     3001 gccggggttg ggcgatcacg ccgtcaccgc tccattcgtc gctggaggga agcccgcgta
     3061 ccgggatgtc gttgggcagg tggaacaacc acggcccgcg gatgcgggcg tagtcattga
     3121 tgccgcgcag cacgtttcgg ccatatgatc gcgtggtctc gatcaatagc gcgacgttcg
     3181 gatatttttt gcgcatccgc cgccgcggcg gcgattcgga ggggctggtt ttcgtgcggg
     3241 gcatggagat ttgacagaca tgatacaatg gaagccaagc cggtactaac gtttatcgcg
     3301 ctgatgccgg cgc
//
LOCUS       DATIMH010000450         4780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5016164, whole genome shotgun sequence.
ACCESSION   DATIMH010000450 DATIMH010000000
VERSION     DATIMH010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5016164"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..944
                     /locus_tag="VLI90_07730"
     CDS             147..944
                     /locus_tag="VLI90_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitosanase"
                     /protein_id="HSV14133.1"
                     /translation="MKRSTIMFPVMLALLLAAGPTTAPSSSVKPGLTAPAKKEIAMQL
                     VSAAENSSLDWRAQYAYIEYNVEHNARENRGYTAGIIGFTSRTHDMLEVVRLYDTISP
                     KNVLSKYLPALEKVDDTPATTGLGKDFERDWKMAGADPKFREAQDRERDRTYFDPTVS
                     QAQADGLHELGQFAYYDCAVMHGSCEHVRAAAMKKAKTPAQGGDEVAYLNAFLDARVR
                     EMKREEAHGDVSRVETAQRVFLREGNLSLDPPLRFKTYGDEYTIPGR"
     gene            complement(1159..1974)
                     /locus_tag="VLI90_07735"
     CDS             complement(1159..1974)
                     /locus_tag="VLI90_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14134.1"
                     /translation="MTLAVTHAPARARPLVKHTTTGQTLLLTATITPPADAVKLARTD
                     PAARLGDYLQALQFYLRLPDDSIDRIVFVENSGSDVSKLRALAYRYPNKQVEFVTFYG
                     LDYPAAYGRAFGEARLIDYAFEHSDILYQVEDDEPIWKGTGRLRLTNLAHMLRRAPRD
                     YQLYCDLRNVNAQWMDQRFYSFTPFGYRALLKNTAEQLREDLHNTLAAETLMYRHVIK
                     HVGPGRVVPRFRSQPFIAGISGFGNTDYGRGFKNHVKDYLKVCSRRLRPSYWI"
     gene            2070..2948
                     /locus_tag="VLI90_07740"
     CDS             2070..2948
                     /locus_tag="VLI90_07740"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV14135.1"
                     /translation="MISTTKSVRDAMPEDLPQVHMPAQAIPQLLKGQRALVTGANSGI
                     GKAVAIALGQAGADVVVNYVRGDEPAEQVVAEASKLGSRAYAHQTDVSNEDQVRGMFQ
                     RMISTFGTIDILINNAGLQQDAPIDTMTLQQWNTVIGINLTGQFLCAREAIREFKRRG
                     VKPDVSVAAGKIICMSSVHEVIPWAGHVNYAASKGGVMLMMKTLAQECAPFRIRVNSI
                     GPGAIRTPINKSAWETPEAYRELLKLIPYKRIGEPEDIAKAAVWLASDQADYITGTTL
                     FIDGGMTLYPGFETGG"
     gene            3133..3873
                     /locus_tag="VLI90_07745"
     CDS             3133..3873
                     /locus_tag="VLI90_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSV14136.1"
                     /translation="MPYVYKHRRPPEPWREFRHKTRHAVLLPFFAMEYSFEWLAYLLS
                     QWSLLEVLEYAGSFSILIAVIFYFAESGERTQARHYQAWQVINTAQGKGGSGGRIDAL
                     QALNDDGVALVGVDVSKAALPGVRLDRADLHRGNFQEADMRDARLHGANLEFADLYNA
                     NLRSADLRGANLRDADLRLADLANANLAGADLSNAKLADADLTGVSNWQQATVEGAHL
                     FGVDHPPDGFVAWAKQHGAIVEEPTTQR"
     gene            complement(4219..>4780)
                     /locus_tag="VLI90_07750"
     CDS             complement(4219..>4780)
                     /locus_tag="VLI90_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14137.1"
                     /translation="PAASAGAGDEVRAPHTPALMFYLTGATESRDADAIRAAVGKLKI
                     ATVLEVNTQRGYAHIRFDSHVVSYHQVAQAIMDAGPSLGKQYDPYLIFRVPDYAKADN
                     AEHVDAIFAGKKLRTRVNVTPIDKSQGEFAVHFLPLKIDPAVTAPQGFNGGHLHHPVS
                     DPAPHGLALESSYEEHIRSCGPAFLS"
BASE COUNT          951 a         1404 c         1578 g          847 t
ORIGIN      
        1 tgctacatca attcgcctca cagacgaaat gcttcgcctt tccattgcta tggcgatgct
       61 gctacaagcg cgatgaacat agatgcccga gcgattgtcc atcaaaagat gggctaggct
      121 ttcagctgcg gcaggagaca ggcacgatga agcgatcgac aatcatgttt ccggtgatgc
      181 tggcattgct cctcgccgcc gggccgacca ctgcgccttc ttcgtccgtc aaacccggtc
      241 ttacggctcc cgcgaagaag gaaatcgcga tgcagttggt gtcggcggcg gagaattctt
      301 cgctcgactg gcgcgcgcaa tacgcctaca tcgaatacaa cgtcgagcac aacgccaggg
      361 aaaaccgcgg gtacaccgcg gggatcatcg ggttcacctc gcgcacgcat gacatgctgg
      421 aagttgttcg gctgtacgac acgatctcgc cgaagaacgt gctgtcgaaa tatctgccgg
      481 cattggagaa ggtggacgac acgccggcga ccacggggct ggggaaggat ttcgagcgcg
      541 actggaagat ggccggggcg gatccgaagt ttcgcgaggc gcaggatcgc gaacgcgatc
      601 ggacctattt cgatccgacg gtctcgcagg cgcaggccga cgggctgcac gagttgggac
      661 agttcgcata ttacgattgc gcggtgatgc acggcagctg cgagcacgtg cgcgcggcgg
      721 cgatgaagaa ggcgaagacg ccggcgcagg gtggggacga ggtggcctat ctgaacgcat
      781 tcctggatgc gcgggtgcgc gagatgaagc gcgaggaagc gcacggcgac gtgagccggg
      841 tggagacggc ccagcgggtt tttctgcgcg aggggaattt gtcgctggac ccgccgctgc
      901 ggtttaagac gtacggggat gagtacacga ttccggggcg gtgacgcgta gcacgggcgt
      961 ctcgcccgtg gtgcgtgtac tcacgcatgg cgatggtgcg ccatcgacga agcaagagcg
     1021 cagcgttacg agcaccaagg cgtagacgct gcgctcttgc tttgtcggcg gtgtaccgcc
     1081 gacattcgtg gcgtaccacg aaacacgggc gagacgcccg tgctacttac gggccagacg
     1141 cccgtggtac atttgaattc aaatccaata actcggccgc aaccgccgcg agcacacctt
     1201 caggtaatcc ttcacgtgat tcttgaatcc gcggccgtag tcggtgttgc cgaaaccgga
     1261 gatgccggcg atgaacggct gcgatcggaa ccgcggcacc acgcggccgg ggccgacgtg
     1321 cttgatcacg tggcggtaca tcagcgtctc ggcggccagc gtgttgtgca ggtcttcgcg
     1381 cagctgctcg gcggtgttct tcaatagcgc gcggtaaccg aacggcgtga aggagtagaa
     1441 gcgctggtcc atccactggg cgttcacatt gcgcaggtcg cagtagagtt ggtagtcgcg
     1501 cggcgcgcgg cggagcatgt gtgcgaggtt cgtcaaccgc agccggccgg tgcctttcca
     1561 gatcggttca tcgtcttcga cctgatagag aatatcggag tgctcgaacg cgtaatcgat
     1621 caggcgggcc tcgccgaagg cgcggccgta ggcggcgggg tagtccaggc catagaacgt
     1681 gacgaattca acctgcttat tgggatatcg atacgccagc gcgcggagct ttgagacgtc
     1741 actgccggaa ttttcgacaa agacgatacg atcgatcgag tcgtccggta gccgcaggta
     1801 gaactgcagc gcttgcagat aatcgcccaa tcgggcggcg ggatcggtgc gggccagctt
     1861 caccgcgtcg gccggtgggg tgatggtggc ggtgagcagc aacgtttgcc cggtggtcgt
     1921 gtgcttgacc agtgggcgtg cgcgtgcagg cgcgtgggtg acggctagcg tcatattctt
     1981 ccctatcggg tgaacaaatg tgttactttt gctccatcaa tcgcatcaaa ggccgcgtgt
     2041 gatgcgatca acccacagga gcagtacaga tgatttcaac gaccaagtca gtccgcgacg
     2101 ccatgcccga agacctgccg caggtgcaca tgccggcgca ggcgattccg cagctgctca
     2161 aagggcagcg cgcgctggtg accggcgcca acagcggcat cggcaaagcc gtggcgatcg
     2221 cgctgggtca ggccggcgcg gacgtcgtcg tgaactatgt ccgcggcgac gagcccgccg
     2281 agcaggtcgt ggccgaggca tcgaagctcg gctcgcgcgc ttacgcgcat caaaccgacg
     2341 tgtcgaacga agaccaggtg cgcgggatgt tccagcggat gatctcaacc tttggcacga
     2401 tcgacatcct gatcaacaac gccgggctgc agcaggatgc gccgatcgac acgatgaccc
     2461 ttcagcagtg gaacacggtg atcgggatca acctcaccgg tcaattcctg tgcgcgaggg
     2521 aggcgattcg cgaattcaaa cgccgcgggg tgaaaccgga cgtgagcgtg gccgccggca
     2581 agatcatctg catgagcagc gtgcacgaag tgatcccctg ggcgggccac gtgaactacg
     2641 ccgccagcaa gggcggcgtg atgttgatga tgaagacgct ggcgcaggaa tgcgcgccgt
     2701 ttcgcatccg cgtgaacagc atcggccccg gcgcgatccg cacgccaatc aacaagagcg
     2761 cgtgggaaac gcccgaagcg tatcgcgagc tgctgaagct gattccgtac aagcgcatcg
     2821 gcgaacccga agacatcgcc aaggccgcgg tgtggctggc cagcgaccag gcggattaca
     2881 tcaccggcac cacgctgttc atcgacggcg ggatgacgtt gtaccccggc ttcgaaacgg
     2941 gcgggtgaac gtgcggaatg acgaaacccg aagtccgaat cccgaatgaa tgacgaatga
     3001 caaaagtacg aatgacgcaa ggcgcggaga agctctgcct gggctaaccg cttcagcggt
     3061 tagtgcatcc attcgtcatt cttcattcga atttcattcg gcgattcgga cttcgccatt
     3121 cggattttcc ccatgcccta cgtctacaaa caccgccgcc cgccggagcc ttggcgtgag
     3181 tttcgccaca agaccaggca cgcagtgctg ctgccattct tcgcgatgga gtacagcttc
     3241 gaatggctcg cgtacctgct cagccaatgg tcgctgttgg aagtgctcga atacgccggc
     3301 agcttttcga tcctcatcgc cgtgatcttc tacttcgccg aaagcggcga gcgcacgcag
     3361 gcgcgccatt accaggcgtg gcaggtgatc aacacggctc aaggcaaagg cggcagcggc
     3421 gggcggatcg acgcgctgca ggcgctgaac gatgacggcg tggcgctggt gggtgtggat
     3481 gtgtcgaagg ccgcgctgcc cggcgtgcgg ctggatcgcg ccgacctgca tcgcggcaac
     3541 ttccaagagg cggacatgcg cgacgcgcgc ctccacggcg ccaatcttga gtttgccgac
     3601 ctctacaacg caaacctgcg cagcgccgac ttgcgcggcg cgaacctgcg cgacgctgat
     3661 ctacggctgg ccgatcttgc caacgcgaac ctcgccggcg ccgatctgtc gaacgcaaag
     3721 ctcgctgatg ccgacctgac cggggtatcg aactggcagc aggcgacggt tgaaggcgcg
     3781 cacctatttg gcgtcgatca tccgccagat ggttttgtcg cctgggcaaa gcagcatggt
     3841 gccatcgtcg aagaaccgac gacgcagcgg tgaacatccc cgcggagatg acgcgaataa
     3901 ccggggctgc gcaggtgtac gcgccgaagt cgaaggatct ggcgggaagg gtccatgaac
     3961 gatcgttgcc ggcgatcgcc gcggcagtcg cttgcgcttc gcggaaacac cgcggcgaat
     4021 ctgcagcgct gccaagtttt tccgaatgcg ccaggaacga tgaatttcgc aacgaccgtc
     4081 gttgaagaaa cgcatcccac gccgggttcg gagcgtactc gcgacggatg tgtcgtgatc
     4141 gagaagaatc cgggtcgtcg aagtattctt cgacttcgct caagacaggc ctccgaaccc
     4201 ggcgtggagg cgggcgagct aggagagaaa cgccgggcca caagagcgta tgtgctcttc
     4261 atagctggac tccagcgcca agccgtgcgg cgcggggtcg ctgaccgggt gatgcagatg
     4321 cccgccgttg aagccctgcg gggccgtcac cgcggggtcg atcttcagcg gcaaaaaatg
     4381 cacggcgaac tcgccctggc tcttgtcgat cggcgtcacg ttcacgcgcg tgcgcagctt
     4441 cttgccggcg aagatcgcat cgacgtgctc ggcgttgtcc gccttggcgt aatccggcac
     4501 gcggaagatc aggtacgggt catattgttt gcccagcgac ggcccggcgt ccatgatcgc
     4561 ctgtgcgact tgatgatagc tgacgacgtg cgaatcgaag cggatgtggg cgtagccacg
     4621 ctgcgtgttc acctcgagca ccgtcgcgat cttcaattta ccgacggcag cgcggatggc
     4681 atcggcgtcg cgcgattcgg tcgccccggt caaataaaac atcaacgccg gcgtgtgcgg
     4741 ggcgcgcact tcatcgcccg cgcctgccga ggccgcgggg
//
LOCUS       DATIMH010000451         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_685596, whole genome shotgun sequence.
ACCESSION   DATIMH010000451 DATIMH010000000
VERSION     DATIMH010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685596"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VLI90_07755"
     CDS             complement(<1..918)
                     /locus_tag="VLI90_07755"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HSV14138.1"
                     /translation="MIDDAAAATTAAPAAAAASASVDPFAVTPPATLGATKDGDTALT
                     NPSVVPLGDSVPSTNPTTAPAANAGQSISASQVSVSDAGTVEIHVNDANLLEVLRMLS
                     LQSQRNIIASKEVTGTVTANLYNVTVREALDAILHANGYAYRERDNFIYVYTAKELIE
                     MDKEARQLSTQVFHLYYTPAANAVNMIKPVLSTESQVAFTAAATSGIDQTGTKDTGGN
                     SHATEDTIVVTDYTDNLEKVKRIIKDIDRRPQQVLVEATILSATLSDNNALGVDFSVM
                     GGVKFDQILGTPASTINSASTGNLTSGAGG"
     gene            complement(1203..1958)
                     /locus_tag="VLI90_07760"
     CDS             complement(1203..1958)
                     /locus_tag="VLI90_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14139.1"
                     /translation="MNQATVRSSMQAVAAIYRRDPKRAGLITAATVAMLGIGARVLFS
                     SPARASAMVVRIDTGSEGFRRPGSSALQNPGLRSALDTGVGVALDPAAWSSWLNAERH
                     APRRNLFGRAAESGSSEYLRTRRGERGDVDQVIAPASVPTAVMVSQTAPQTKETGGIW
                     DEVAKSMAAQADQNMERQRSHLVFLRRQAGELRLTQYVPGPPPAAWVNGELVGEGSVV
                     ASFRVLTIEPRRIIVEREGILLEIPLNQATMPK"
     gene            complement(1955..2518)
                     /gene="pilO"
                     /locus_tag="VLI90_07765"
     CDS             complement(1955..2518)
                     /gene="pilO"
                     /locus_tag="VLI90_07765"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HSV14140.1"
                     /translation="MLDLKDQLLWCRRAQWALAAGLLLGVGVTYLAAIRPASLRLSVV
                     AARATAQQAKLDVARARLQTLADVERDTQQLREVLERADKQLLQSGELPRVIAEVSQL
                     GKDSLLRNVQWRADARPTRTQRFTELPVEFSFAGRFANVFQFLRQTEQMQRLTRVKRL
                     SIHTGDSGDADPQVDAQLTMNLYFVDE"
BASE COUNT          450 a          916 c          748 g          481 t
ORIGIN      
        1 tccgcccgcg ccgctggtca ggttgccggt cgaagctgaa ttgatcgtgc tggccggcgt
       61 acccaggatc tggtcgaact tcacgccgcc catcaccgag aagtccacgc ccagcgcgtt
      121 gttgtcgctc agcgtcgcgc tcaggatcgt cgcctccacc agcacctgct gcggccggcg
      181 gtcgatgtcc ttgatgatcc gcttcacctt ctccaggttg tcggtgtagt cggtgacaac
      241 gatcgtgtct tctgtcgcgt gtgaattgcc gccggtgtcc ttcgtgcccg tctggtcgat
      301 gcccgacgtc gccgccgccg tgaacgcaac ctgcgactcc gtgctcagca ccggtttgat
      361 catgttcacc gcattcgcgg ccggtgtgta atacaggtgg aacacctgcg tgctcagctg
      421 acgagcttcc ttgtccatct cgatcagctc tttggccgtg tacacataga tgaagttgtc
      481 gcgctcgcga taggcgtagc cgttggcgtg caggatcgcg tcgagcgctt cgcgcaccgt
      541 gacgttatac aggttggcgg tcaccgtgcc ggtgacttcc ttgctggcga tgatgttccg
      601 ctgcgactgc agcgagagca tccgcagcac ttcgagcagg ttcgcgtcgt tgacgtgaat
      661 ctcaaccgtg ccggcgtcgc tgacgctcac ctgggaagcg ctgatgctct gcccagcgtt
      721 agccgcgggc gcagtggtcg ggttggtgct cggcacgctg tcgccaagcg gcacgacgct
      781 gggattcgtg agcgcagtat ccccatcctt ggtcgcgccg agcgtcgctg gtggagtcac
      841 cgcgaacgga tcgaccgagg ctgaggcggc agccgctgcg ggtgcggcgg tcgtcgcggc
      901 agccgcgtcg tcaatcatct tgacgtcgtt ctttgccgcg ccttccttgc ctaccgtgac
      961 tgccgcaaat agccgattcg ccggaccgga ataaacggcc agcgaggctc caaaaaaaag
     1021 accagcgatt ccggtccagg tccgcataat caacctcgtg ttgatcgccc tcgcgtcggt
     1081 tttgaaatgt gcgttaccgg attgacctgg gaatgcacca atcgccagag acggaaagcc
     1141 ggctcgcgcg gatcgggtcc cagatcgccg ctgtgcacaa acaaaagcgc cacgcgcacg
     1201 atctacttgg gcatcgtcgc ttggttcaac ggaatctcga gcaagatgcc ctcgcgctcg
     1261 acgatgatcc gtcgcggctc aatcgtgagc acgcgaaacg acgcgacgac actaccctct
     1321 ccaaccaact caccgttcac ccaggccgct ggcggcggtc cggggacgta ctgcgtcagc
     1381 cgcaactcac ccgcctgcct ccgcaggaac acaaggtgac tccgctgccg ctccatgttc
     1441 tgatcggctt gagccgccat cgactttgcc acttcatccc aaattccccc ggtttcttta
     1501 gtttgtggcg ccgtttgaga aaccataacg gctgtaggga cggacgcagg cgcgataacc
     1561 tgatcaacgt ccccacgctc cccacgccgg gttcggaggt actccgacga cccggattcc
     1621 gccgcgcgcc caaacaagtt ccggcgcggc gcatgacgct ccgcattcaa ccaggaagac
     1681 caggcagcag gatcaagcgc caccccgacg ccggtgtcta aggcactccg aaggcccggg
     1741 ttctgcagcg ccgaagatcc aggtcttcgg aatccctcag acccggtgtc gatacgcacc
     1801 accatcgcgc tcgcccgcgc cgggctgctg aacaacacgc gcgcgccgat ccccaacatc
     1861 gccaccgtcg ccgcggtgat cagcccggcg cgtttgggat cgcgccggta aatcgccgcc
     1921 accgcctgca tcgagctgcg caccgttgcc tggttcattc gtccacgaag taaaggttca
     1981 tcgtcagctg cgcatccacc tgcggatccg cgtccccact gtcgccggta tgaatgctga
     2041 gccgcttcac ccgcgtcagc cgctgcatct gctccgtctg ccgcaggaat tggaacacat
     2101 tggcaaatcg ccccgcgaag gagaactcca ccggcagctc ggtgaaccgc tgggtgcgcg
     2161 tcggccgggc gtcggcgcgc cactgcacgt tccgcagcag cgaatccttc cccagctgcg
     2221 acacctccgc gatcacccgc ggcaactccc cgctctgcaa taactgcttg tccgcccgct
     2281 ccagcacttc gcgaagctgc tgcgtatcgc gctccacatc cgccagcgtc tgcaaccgcg
     2341 cccgcgcgac gtccagcttc gcctgctggg ccgtcgcccg cgctgcgaca acgctcaacc
     2401 gcaaagacgc cggccgaatc gccgccaggt acgtgacccc cacgcccagc agcaaccccg
     2461 cggcaagcgc ccactgcgcc cgccggcacc acagaagttg gtccttcaaa tcgagcatga
     2521 ttacttcgac aaccccccga cgccggtgtc tgaggtcctc cgaagaccct gcttctgcag
     2581 cgccgaagct tgcgc
//
LOCUS       DATIMH010000452         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4435785, whole genome shotgun sequence.
ACCESSION   DATIMH010000452 DATIMH010000000
VERSION     DATIMH010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4435785"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VLI90_07770"
     CDS             <1..1290
                     /locus_tag="VLI90_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HSV14141.1"
                     /translation="AAGAAAGMAATFGSPVSAVLLAVELLLFEFRPRSFIPVALAATT
                     AAGIRFIMGDAYPIFDMPALHNPTGWAMLAYFLIGGLTGVVSVGVTRAVYWIEDMFEH
                     LPVHWMWWPAIGAIPLGIIGYFRPDVLGVGYSNITLTLSGERTLGFLAMLGLMKFIAW
                     AIALGSGTSGGTLAPLFTIGGALGGVCGGAMSVWWPHAGIDARVGALVGMAAMFSGAS
                     RALLTSVVFAFETTLQPLGMLPLLGGCTAAFFASCVLMPNTIMTEKIARRGVRVPAEY
                     EADYLHGIAVSEVVSPDIVTLPADWTVQRVREWIDSAIDGAAHQGYPVVDERGHLVGV
                     LTRRNLLDPATPRDRKLAELIHRPPVVVYPDVSLREAADHMVNHDIGRLPVIDRATAK
                     VIGMITRSDVLGAHGRRLRDAQQAPQSIHVPRVQQTA"
     gene            1504..2247
                     /locus_tag="VLI90_07775"
     CDS             1504..2247
                     /locus_tag="VLI90_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HSV14142.1"
                     /translation="MARFPQSPFGAGGGGFAGGETLPYRSKEQQVTLGQFFNAVYAWM
                     AVGLALTAVVAFVVATYARSMMLNPGVLIISVIAQFALVITISRAINRINASVATGLF
                     LVYAALNGVWLSVLFALYAHATLASTFMVTAGAFGATSLYGYTTKRDLTGLGSLLFMA
                     LIGLILASLVNLFFMNPMMQWLISYAGVLIFVGLTAYDTQNLKAIAFQTGDNPALAHR
                     MAIVGSLRLYLDFINLFLFLLEIMSDRRR"
     gene            complement(2320..>2621)
                     /locus_tag="VLI90_07780"
     CDS             complement(2320..>2621)
                     /locus_tag="VLI90_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14143.1"
                     /translation="VAERVVPADQLASTGKDAKSRLVSLASILSDAGIPIDVESYPGY
                     AIVRGCSCPLSAASAQHPQLCDADATLMARTLGCTVRQCCDRANQPRCGFRVAWK"
BASE COUNT          405 a          799 c          909 g          508 t
ORIGIN      
        1 gccgccgggg cggccgcggg catggcggcg acgttcggtt cgccggtgtc ggcagtgttg
       61 ctcgcggtcg aactgttgct gttcgaattt cgcccgcgat cgtttatccc cgtcgcgctg
      121 gccgccacca cggcggcggg catccggttc atcatgggcg atgcatatcc gatcttcgac
      181 atgccggcgc tgcacaatcc caccggctgg gcgatgctgg cgtacttcct catcggcggg
      241 ctcaccggcg tcgtctcggt tggcgtgacg cgcgcggtct actggatcga agacatgttc
      301 gagcacctgc cggtgcactg gatgtggtgg ccggccatcg gggcgattcc gctgggcatc
      361 atcggatact ttcggcccga cgttctgggc gtggggtatt caaatatcac gctcacatta
      421 tcgggtgagc gcacgctggg gttcctggcg atgctcggtc tcatgaagtt catcgcctgg
      481 gcgatcgcgc tgggcagcgg aaccagcggc ggcacattgg cgccgttgtt tacgatcggc
      541 ggcgcgctgg gcggcgtgtg cggtggcgcc atgtcggtct ggtggccgca cgcagggatc
      601 gacgcgcgcg tcggcgcgct ggtcggcatg gcggcgatgt tctccggtgc atcccgcgcg
      661 ctgctgacat cggtcgtctt cgcgttcgaa acgacgctgc agccgctggg catgctgccg
      721 ttgctgggcg ggtgcacggc ggcgttcttc gcatcttgtg tgctcatgcc gaacacgatc
      781 atgacggaaa aaatcgcccg ccgcggcgtg cgcgtgccgg cggaatatga ggccgattac
      841 ctgcacggca tcgcggtcag cgaggtcgtc agtccggaca tcgtcacgct gccggcggac
      901 tggacggtgc agcgcgtgcg cgagtggatc gacagcgcga tcgacggcgc ggcgcaccag
      961 ggttatccgg tggtcgacga gcgcggccac ttggttggcg tgctgacccg gcgaaacctg
     1021 ctggacccgg cgacgccgcg ggatcgcaaa ttggcggagt tgatccatcg cccgccggtg
     1081 gtggtgtatc cggacgtctc gctgcgcgag gcggcggacc acatggtcaa ccacgacatc
     1141 ggccggctgc cggtgatcga ccgtgcaacg gcgaaggtga tcgggatgat cacgcgcagt
     1201 gacgtgctgg gggcgcacgg ccggcgcctg cgggatgccc agcaagcgcc gcagtcgatc
     1261 cacgtgccgc gggtgcagca gacggcttga tcaatatgcc gttgttggtg aatccgatcg
     1321 acgccggtgt ctgaggtacg ctgatgaggt acgctgaaga cccgggtctt cggagtacct
     1381 cagacaccgg cgtcgatcgg cgcggtggtg attctaaaac ccgcgcatct ttcgattggc
     1441 gctcgccgcg cccgtggcat acaattcccc ggtccaatta ccaattaagc aaggagtcca
     1501 aagatggcac gatttccaca aagccccttc ggggccggcg gcggtgggtt cgccgggggt
     1561 gaaacactcc cgtatcgatc taaagaacaa caggtcacgc tcgggcagtt cttcaacgcg
     1621 gtctatgcat ggatggcggt gggcctggcg ctgacggcgg tcgtcgcgtt cgtcgtcgcg
     1681 acctacgcac gatcgatgat gctcaacccc ggcgtcttga tcatctcggt cattgcgcag
     1741 ttcgcgctgg tcatcacgat cagccgggcg atcaaccgca tcaacgcatc cgtggcgacc
     1801 gggctgttcc tcgtctacgc ggcgttgaac ggcgtctggc tgtcggtact gttcgcgctt
     1861 tatgcccacg ccacgctggc cagtacgttc atggtgacgg ccggggcgtt cggcgcgacc
     1921 agcctgtacg gctacacgac caagcgcgac ctgacggggc tgggcagctt gctgttcatg
     1981 gccctgatcg gcctgatcct ggcctcgctc gtcaacctgt tcttcatgaa cccgatgatg
     2041 caatggctga tcagctacgc cggggtgctg atcttcgtcg ggctgaccgc gtatgacacg
     2101 cagaacctga aagcgatcgc gttccagacc ggggacaacc cggcgctggc gcaccgcatg
     2161 gcgatcgtcg ggtcgctgcg gctgtacctc gatttcatca acctgttcct gttcctgctg
     2221 gagatcatga gcgatcgccg gcggtgaacg atcgcgggac gcgatttgaa gtgagatgca
     2281 caccgggagc cccgcatgtc catgtggggc ttcttctttt tatttccacg cgacgcggaa
     2341 accgcagcgg ggttggttgg cgcgatcgca gcattgcctg acggtgcagc cgagcgtgcg
     2401 ggccatcagc gtggcgtcgg cgtcgcaaag ctgtgggtgc tgggcggagg cggccgacag
     2461 cgggcagctg cacccgcgga cgatcgcgta gccgggataa ctttccacgt cgatgggaat
     2521 gccggcgtcg ctgaggattg acgcgagcga cacgaggcgc gacttggcgt ctttgcccgt
     2581 ggacgcgagt tgatcggcgg ggaccacgcg ctcggccacc t
//
LOCUS       DATIMH010000453         4274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1550073, whole genome shotgun sequence.
ACCESSION   DATIMH010000453 DATIMH010000000
VERSION     DATIMH010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550073"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VLI90_07785"
     CDS             <1..555
                     /locus_tag="VLI90_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14144.1"
                     /translation="GWTEWPLTAEKFAAWVNNINGDGFLCNLFMDYETFGEHQWADTG
                     IFQFLSSLPEKIFDVAKGQNHFVTPSMALKEFKPVGEVDVPHMISWADTERDLSAWLG
                     NAMQSNALHELYKLEGPLKERGDEQLLSDWRKLTTSDHFYYMCTKYWADGEVHKYFNP
                     YESPYDSYINFMNVLDNVQSRLRE"
     gene            592..714
                     /locus_tag="VLI90_07790"
     CDS             592..714
                     /locus_tag="VLI90_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14145.1"
                     /translation="MPKQANYLRHEQLEQVLSPTRLALPARPRVEEIKVEEYQD"
     gene            736..915
                     /locus_tag="VLI90_07795"
     CDS             736..915
                     /locus_tag="VLI90_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14146.1"
                     /translation="MILADDAPEDETLWAMVQPTFAIIRQALQGAGETRFAYISAGTR
                     ADYAARNAVNADGDD"
     gene            complement(1428..2378)
                     /locus_tag="VLI90_07800"
     CDS             complement(1428..2378)
                     /locus_tag="VLI90_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HSV14147.1"
                     /translation="MADTTKSILGLHHVTAITADAQKNIDFYSGVLGLRLVKLTVNFD
                     DPSSYHLYYGDELGRPGTIMTFFAWPGAHRGRIGPPQVSVTAFAVPMGTIDFWADRLK
                     ERSVVSQRAADRFGERVLSFADPDGLQLEIIETNNTAGQPWAAGPVALGQAIRGFHSV
                     TIAEEGYEKTAQLVIGLMGFTAAGAEQNRFRYRAAAADAFAAHIDVVCVPAAPHGTMG
                     AGVVHHVAFRAANDPQQVEWQRAIADAGFNVSPVMDRTYFHSIYYREPGGVLFEIATD
                     SPGFTADQPAAELGTRLMLPPWLEPHRKEIERAVTPVKLP"
     gene            complement(2406..3053)
                     /locus_tag="VLI90_07805"
     CDS             complement(2406..3053)
                     /locus_tag="VLI90_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSV14148.1"
                     /translation="MNGGPHAKIPTATAGPKPGQARATLILLHGRGATAQSMLSLHEE
                     LAIETVAAIAPQAAGHTWYPQSFLAPIEMNQPYLDSALARIDSIVSDLLARKIASERI
                     AILGFSQGACLTCEYVARHPRRYGAAMGLTGALIGPPGTPRNYAGSLDGTPVFLGTSD
                     PDPHVPYERVLETRGVFTRMGATVDLRRYPGMPHTINEEELDACRALVQQIVNAA"
     gene            3092..3748
                     /locus_tag="VLI90_07810"
     CDS             3092..3748
                     /locus_tag="VLI90_07810"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HSV14149.1"
                     /translation="MHENTTARVEAFSDGVFGIALTLLVLDFKVPHLGDQSAITSRQL
                     TRELLHMWPTIIAFVLSFGTVLTMWINHHGLFRHAHRTDNRLLFANGLLLLVVTFVPF
                     PTAVLADYLDRPAANAATAFYCGTFILINIGYNLLLRAIEVNRHPTPTPSEDAAFNGI
                     RRTYYITFGVYLLATIMALWNAYVGLAICLSLWLVWALLKYEAGHRSLNPNDEVRIPE
                     "
     gene            complement(3901..>4274)
                     /locus_tag="VLI90_07815"
     CDS             complement(3901..>4274)
                     /locus_tag="VLI90_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14150.1"
                     /translation="TIIQTIPALLLSAVDREDPVRVGDQVHYTITVQNQGSGDDSDVN
                     VKAIVPDEQEFVSANGVTRGNVDGRTITFETIPTLGAGKSVQWEVTTKALRPADTIFR
                     VEATSKSITKPATKEEPTKLY"
BASE COUNT          802 a         1349 c         1271 g          852 t
ORIGIN      
        1 ggctggaccg agtggcccct caccgccgag aagttcgccg cctgggtcaa caacatcaac
       61 ggcgacggct tcctctgcaa cctcttcatg gactacgaaa ccttcggcga gcaccagtgg
      121 gccgacaccg gcatcttcca gttcctctcc agcctcccag aaaaaatctt cgacgtcgcc
      181 aagggccaga accacttcgt cacccccagc atggccctca aagagttcaa gcccgtcggc
      241 gaagtggatg ttccgcacat gatcagctgg gccgacaccg agcgagatct cagcgcctgg
      301 ctaggcaacg cgatgcagtc caacgcgctg cacgagctgt acaaattgga aggtccgctg
      361 aaagaacgcg gcgacgagca gcttctatcc gattggcgca agctgacgac gagcgatcat
      421 ttctactaca tgtgcacgaa gtactgggcc gacggcgaag tgcataaata tttcaatccg
      481 tacgagagcc catacgacag ctacatcaac ttcatgaacg tgctggataa cgtacagtcg
      541 cggctgcggg aatagtcagt tgaactttgc tataatcagc gcagggacat catgccaaag
      601 caggcgaact atctgcggca cgaacagctg gagcaggtgc tgtcgccaac tcgactcgcg
      661 cttcccgctc ggccgcgtgt tgaagagatc aaagtcgaag agtatcagga ctgagacgac
      721 tcgctcgata ttttcgtgat acttgccgac gatgcgcctg aagatgaaac cctgtgggcg
      781 atggttcaac ccaccttcgc gatcattcga caagcgttgc agggcgccgg ggagactcgc
      841 tttgcgtata tctcggccgg cacccgcgcc gactatgccg ctcgcaatgc tgttaacgcc
      901 gacggagatg attaacaccg atgccgctgg ccgaccagct gattgaccaa gcgcgacatc
      961 tggccacgcg cgatatgcaa ggccgcccgc gacaaggcaa cttgcgccgc gccacctccg
     1021 ccgcttacta tgcgctgttc cattttctga tcgatcaggc atgccgttca ctcgtgggcg
     1081 gcgcgcctaa tcgacggcga ctgcgcggtg tacttgcgcg tgcattcgat catgcggcga
     1141 tgaaacgagc ggcaacgagt ttcgcaagcg ggacccttcc cgcgcgattc gtccctgccc
     1201 tacccggtgg atcgatatct ccagaacttc gcgaattagc cacggcacgt ggctgactat
     1261 gacctcttgc gcacgccagg tcgccgcgag gtgttactcc tcatcgcgca gtcagagaac
     1321 gccatgcgtc tttggcagca ggtgcaggag agcgacgggg ccgactgttc cttctctcgc
     1381 tgctggtcaa cgatcgtgta cgggaatgga ccgcaacttc ttcgtggtta tggcaacttt
     1441 accggtgtca ctgcccgttc aatctccttc cgatgcggct ccaaccacgg tggcagcatc
     1501 agccgcgtgc ctagctccgc cgccggttga tccgccgtga acccggggct gtcggtcgcg
     1561 atctcgaaca gcaccccgcc tggctcgcgg tagtagatcg agtggaagta cgtccggtcc
     1621 atcaccggtg agacgttgaa accggcgtcc gcgatggcgc gctgccattc gacctgctgc
     1681 gggtcattgg cggcgcgaaa cgcgacgtga tgcaccacgc ccgcgcccat tgtcccgtgc
     1741 ggcgcagccg gcacacagac gacatcgata tgagccgcga aagcatcggc cgcggcggcg
     1801 cgatagcgga agcggttctg ctcggcgccc gcggcggtga agcccatcaa cccgatgacc
     1861 agctgcgcgg tcttttcgta tccttcttcc gcgatcgtca cgctatgaaa cccgcggatc
     1921 gcctgcccca gcgccaccgg ccccgctgcc cagggttggc cggcagtatt gttcgtttcg
     1981 atgatctcca actgcaggcc gtcgggatcc gcgaagctga ggacacgttc accaaagcga
     2041 tccgccgccc gttgcgagac aacgctgcgc tctttcaatc gatccgccca aaagtcgatc
     2101 gtgcccatcg gcaccgcgaa cgccgtcacg ctcacctgcg gcggaccgat ccgtccgcgg
     2161 tgcgcgccgg gccacgcgaa gaacgtcatg atcgtgccgg gccggcccag ttcatcgccg
     2221 tagtagaggt gatagctgga tggatcatcg aagttgaccg tcagcttaac gagccgaagg
     2281 cccagcacac cggagtagaa gtcgatgttc ttctgcgcat cggcggtgat ggcggtcacg
     2341 tggtgcaggc ccaggattga tttggttgta tctgccatga ttgcattctc tgaagatgtg
     2401 ccaattcacg ccgcgttgac gatctgttgg accagcgcgc ggcacgcgtc cagctcttct
     2461 tcgttgatcg tgtgcggcat gccgggatat cgccgcagat cgacggtcgc tcccatgcgc
     2521 gtgaatacgc cccgcgtctc cagcacgcgc tcatacggca cgtggggatc gggatcgctg
     2581 gtgccgagga acaccggcgt gccatcgagt gaaccggcat aattccgagg cgtgccgggt
     2641 gggccgatca acgcgccggt cagtcccatc gccgcgccat accgccgcgg atgtcgcgcg
     2701 acatattcgc aggtcaggca agcgccctgc gagaagccga ggatcgcgat gcgttcgctg
     2761 gcaattttgc gcgccagcaa atcgcttacg atcgagtcga tccgcgccag cgctgagtcg
     2821 agatacggct ggttcatctc gatcggcgcg agaaaagatt gcggatacca agtatgcccc
     2881 gccgcctgcg gcgcgatggc ggcgacggtc tcaatcgcca gctcctcgtg cagcgacagc
     2941 atgctctggg ccgtcgcccc gcggccatga agcaggatga gcgttgcgcg tgcctgcccg
     3001 ggcttgggcc cggcagtcgc agtcggtatc ttggcatggg gtccgccgtt catttgttct
     3061 ccatcgcccg cccgatacat tcagcaagta tatgcacgaa aatacgaccg cgcgcgtcga
     3121 ggcctttagc gatggcgtct tcgggatcgc cctgacgctg ttggtcctcg acttcaaggt
     3181 cccgcatctt ggagatcaat ccgccatcac atcacggcaa ctgacgcgcg agctgttgca
     3241 catgtggccg acgatcatcg ccttcgtcct cagcttcggc accgtgctga cgatgtggat
     3301 caaccaccac ggcctgttcc gccacgcgca tcgcacggac aaccgcctgc tgttcgccaa
     3361 cgggttgctg ctgctggtcg tgacgttcgt gccgtttccc acggcggtgc tggcggacta
     3421 tctcgatcgg cccgccgcca acgccgcgac cgcgttttac tgtgggacgt ttatcctgat
     3481 caacatcgga tacaacctgc tgctgcgcgc aatcgaggtc aaccggcatc caacgcccac
     3541 accctcagag gatgccgcgt tcaacggcat ccgccggacg tactacatca cgttcggcgt
     3601 ttacctgctg gcgacaatca tggcgctgtg gaacgcgtat gtgggattgg cgatctgttt
     3661 gtcactgtgg ctggtgtggg cactgctgaa gtacgaggcg ggacatcgct cgctaaatcc
     3721 gaatgacgaa gtccgaatcc ccgaatgacg aatgacgaat ggatgcacta accgctaaag
     3781 cggttagccc gggcggagcg gctcaggcgc gcccgtgcga atttggccat tcggatttcg
     3841 tcattcgtca ttcattcggg gcttcgggtt tcggatttcg tcatttgaca aacacagcgt
     3901 tcaatagagc ttcgtcggct cttccttggt cgcgggcttg gtgatcgact tgctcgtcgc
     3961 ttcgacgcgg aagatcgtgt cggcggggcg aagtgccttg gtggtcactt cccactgcac
     4021 gcttttgccg gcgcccagcg tgggaatggt ttcgaaggtg atcgtccggc catccacgtt
     4081 gccgcgggtg acgccgttgg cgctgacgaa ctcctgctcg tccggaacga tcgccttgac
     4141 gttcacatcc gaatcgtcac cactgccctg gttctgcacg gtgatcgtgt agtggacctg
     4201 atcgccgacg cgcaccgggt cttcgcgatc gaccgcgctc agcagcagcg ctgggatcgt
     4261 ctggatgatc gtgt
//
LOCUS       DATIMH010000454         3558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2414329, whole genome shotgun sequence.
ACCESSION   DATIMH010000454 DATIMH010000000
VERSION     DATIMH010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414329"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1591)
                     /locus_tag="VLI90_07820"
     CDS             complement(101..1591)
                     /locus_tag="VLI90_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HSV14151.1"
                     /translation="MAMFKRTRVASLVDAIEARPGEELSPGANGNGNVRRHPQQLVPT
                     DLVPGLSKFDGEEAPVKPSPEAAAQQAYFQSMKQRIHQQLMDRLDVQSLRSVPLDLVR
                     QEVRRVIRDLCQSEKGLLSGADQERLVDEVMDETFGLGPLEPLLKDPAVTDILVNRFD
                     RVYIERKGRLTLSEVRFRDNAHLRQIIDRIVARVGRRVDEFSPMVDARLSDGSRVNAI
                     IPPLSLDGPAMSIRRFGSRPLQLEDLITLGAFPEELFQFLAAAVQARCNILISGGTGS
                     GKTTLLNCMSRFIAADERVITIEDAAELQLQQPHVVRLETRPPNIEGRGEVTPRDLVR
                     NSLRMRPDRIIVGEVRGAEALDMLQAMNTGHEGSMTTIHANSTRDALSRLEVTVTLSG
                     FDIPVRALRQQVASAIHLVVQVQRLLGGRRKVTRVSEITGMEGEQLQMHDIFVFEQTG
                     VSEAGHATGEFRSTGIRPRCLERVEAAGLALPPDLFMRRVLATVRH"
     gene            complement(1604..2719)
                     /locus_tag="VLI90_07825"
     CDS             complement(1604..2719)
                     /locus_tag="VLI90_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSV14152.1"
                     /translation="MFAQLTAAVVDPAPTNREEMVAFLKAHHVEVSASVGALESLEPM
                     LNTAAAPKLVVVTLDPSPRDMLQKVGALIRRHPGTHFFVVSQVVDPKLLMDAIRQGVR
                     EFIPLPVDEDQFRAAVERISADTADAARSRLIHIIPAAGGCGATTIACNVAASLAKKG
                     PTLLIDLDLVRGSVASNFDLRPRYTIADLMQSAERLDRQLVESAIVEHPKSGVSILAR
                     PDVPEESLSVTPVGFNRLLNVVGSEYDYIVVDSVMSVEPLYTSVMKAADLNVLVMELT
                     VPTAHSAERFMKVLQRLGVDQDRTRVVVNRATKRNDIEPADVEKLLKTRLMWSVPNDF
                     RNAVASINVGEPVVLRAPRAELSSSLTGLAGALNGKA"
     gene            complement(2737..>3558)
                     /gene="cpaB"
                     /locus_tag="VLI90_07830"
     CDS             complement(2737..>3558)
                     /gene="cpaB"
                     /locus_tag="VLI90_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HSV14153.1"
                     /translation="DFRTVEAPLELIPDQSLHEVKQTTGRVLCAAITRGQMLTESLLA
                     PIGSSQGLEATIPRGKRAFTVDVSETTGLAGMILPGSRVDIVTTMTDDKTSESRTWTM
                     LQNAPVTAVGTRLSKNGPDEASGKTLVKTVTLLVTPEQAEMLDMAYTRSHPRLVLRNQ
                     AEGEADYADSGITFSEFFHRHETSGTPLGDSMSGLLTRLAAAKQPGLDNDTAAKPAPV
                     AVAVAPAPVVEPPRRSVEVVRGGVSTTVYFDGPGGSTTGGPAVASKTDPFATVRN"
BASE COUNT          597 a         1193 c         1109 g          659 t
ORIGIN      
        1 ttgctaaagc aaccacgccg cgcgggcttt tcgttcctcg atccatccgg gtcgtcgcga
       61 gtacgcttcg aacccggcgt gttgcgcgcg aaggcgttcc tcaatgccgc acagtcgcca
      121 gcacgcgtcg catgaacaaa tccgggggca gcgccagccc ggcggcttct acgcgttcca
      181 gacaccgcgg gcggattccc gtcgatcgga attcgcccgt ggcgtggccg gcttcgctga
      241 cgccggtttg ctcgaagacg aagatgtcgt gcatctgcaa ctgctcgcct tccatgccgg
      301 tgatctcgct gacgcgcgtg accttgcggc gaccgcccag caggcgctgc acctgcacca
      361 ccaggtgaat cgcgctggca acctgttgcc gcagcgcgcg caccgggatg tcgaacccgg
      421 atagcgtgac cgtcacttcc aaccggctta gcgcatcgcg cgtcgagttc gcgtggatcg
      481 tcgtcatcga tccttcatgc cccgtgttca tcgcctgcag catgtccagc gcttcagccc
      541 cgcgcacttc gccgacgatg atccggtccg gccgcatgcg caggctgttg cggaccagat
      601 cgcgcggcgt gacttcaccg cggccttcga tgttcggtgg gcgcgtctcc agtcgcacga
      661 cgtgcggctg ctgcaactgc agttcggccg cgtcttcgat cgtgatcacg cgttcatcgg
      721 cggcgatgaa ccgcgacatg cagttcagca gcgtcgtctt gcccgaaccc gtgccgccgc
      781 tgatcaggat gttgcaccgc gcctgcaccg cggcggccag gaactggaac aactcctcgg
      841 ggaacgcgcc gagcgtaatc agatcttcca gctgaagcgg gcgagatccg aagcggcgaa
      901 tgctcatcgc cggcccgtcc agcgacagcg gcgggatgat cgcgttcacg cggctgccat
      961 cggacaggcg cgcatccacc atcgggctga actcatcgac ccggcgaccg acgcgggcga
     1021 cgatgcggtc gatgatctgc cgcagatgcg cgttatcgcg gaagcggact tcagacagcg
     1081 tgagccggcc cttgcgttcg atgtacacgc gatcgaatcg gttgacgagg atgtcggtga
     1141 cggccgggtc cttaagcagc ggttccagcg gaccgaggcc gaacgtctcg tccatcacct
     1201 catcgaccag ccgctcctgg tccgcgccgc tgagcaaacc tttttcgctc tggcacagat
     1261 cgcggataac ccggcgaact tcctgccgca ccagatcgag cggcaccgag cgcaggctct
     1321 gcacgtccag ccgatccatc agctgctgat ggatccgctg cttcatcgat tgaaaatacg
     1381 cctgctgcgc cgcggcttct ggcgacggtt tcaccggcgc ttcctcgccg tcgaacttcg
     1441 acaagcccgg caccagatcc gtcggcacca gctgctgcgg gtgccggcga acattgccgt
     1501 tcccattcgc gcccggcgac agttcttcac ccggacgagc ctcgatggcg tccaccaatg
     1561 atgcgacacg cgtgcgcttg aacatcgcca tattcaacta cctttacgcc tttccattca
     1621 gcgccccggc cagtccggtc aggctcgaac tcaactccgc gcgcggcgcg cgcagcacga
     1681 ccggttcacc cacgttgatc gatgcgacgg cgttgcggaa atcgttcggc accgaccaca
     1741 tcagccgggt cttcagcagc ttttccacgt cggctggttc gatgtcgttg cgcttggtcg
     1801 cgcggttgac gacgacccgc gtgcgatcct gatccacacc cagccgctgc agcaccttca
     1861 tgaagcgctc ggcgctatgg gcggttggca cggtcaattc catcaccagc acattcaggt
     1921 cggccgcctt catcacggac gtgtacagcg gttccacgct catcaccgaa tcgacgacga
     1981 tgtagtcgta ctcgctgcca acaacgttga gcagccgatt aaaccccacc ggcgtgacgc
     2041 tgaggctttc ctccggcacg tccggtcggg cgagaatcga gacgccgctt tttggatgct
     2101 cgacgattgc gctttcgacc agctgccgat cgagccgctc ggcggactgc atcaggtcgg
     2161 caatcgtgta gcgcggacgc aggtcgaagt tgctcgcgac gctgccgcga accagatcga
     2221 ggtcgatcag cagcgtcgga cccttcttcg ccagcgacgc cgccacgttg caggcgatcg
     2281 tcgtcgcccc gcaaccaccg gcggcgggga tgatgtggat caaccggctg cgagccgcgt
     2341 cggcggtgtc ggcgctgatg cgctcgaccg cggcgcgaaa ctgatcttcg tccaccggca
     2401 gcgggatgaa ctcgcgcacg ccctggcgaa tcgcgtccat cagcagtttt ggatcgacca
     2461 cctgcgagac aacgaagaaa tgcgtgccgg ggtgccggcg gatgagcgca ccgacctttt
     2521 gcagcatgtc gcgcggcgaa ggatcaagcg tcaccaccac cagcttcggg gcggcggcgg
     2581 tgttcagcat cggttccagc gattccagcg cccccaccga cgccgacact tccacgtgat
     2641 gcgctttcag aaacgccacc atctcctcac gattggtggg agcgggatcc acaacggcgg
     2701 cggtcaactg cgcgaacatt ggcgatcgtc cttgattcaa ttacgaacgg ttgcgaacgg
     2761 atcagtcttg ctggcgacgg cgggaccgcc ggtcgtcgat ccaccgggtc catcgaaata
     2821 caccgtggtc gagacgccac cgcgcacgac ttcaacggaa cgccggggtg gttcgactac
     2881 cggagcaggc gccactgcga ccgcgaccgg cgcgggtttg gcggcggtgt cattgtccaa
     2941 tccgggttgc ttggcggccg ccaagcgagt cagcaggccc gacatggaat cacccaacgg
     3001 cgtgccggac gtctcgtgac gatggaagaa ttcgctgaag gtgatgccgg agtcggcgta
     3061 gtccgcctcg ccctccgcct ggttccgcag caccagccgc gggtggcttc gggtgtacgc
     3121 catgtcgagc atttctgctt gctcgggcgt gaccagcagg gtgaccgttt tcaccagtgt
     3181 tttgccgctg gcttcatcgg gcccgttctt ggacagccgc gtgccgaccg ccgtcaccgg
     3241 cgcgttttgc agcatcgtcc aggtgcggct ttcgctggtc ttgtcgtcgg tcatcgtcgt
     3301 gacgatgtcc acgcggctgc cggggaggat catgccggcc aagccggtgg tttcgctgac
     3361 gtctacggtg aacgcgcgct tgccgcgcgg gattgttgcc tcgaggcctt gcgaagaacc
     3421 gattggcgcc agcagcgatt cggtcagcat ctgcccacgc gtgatggccg cgcacagcac
     3481 gcggccggtg gtctgcttca cttcgtgcag ggactgatcg gggatcaatt cgagcggcgc
     3541 ctcgacggtg cgaaaatc
//
LOCUS       DATIMH010000455         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2993413, whole genome shotgun sequence.
ACCESSION   DATIMH010000455 DATIMH010000000
VERSION     DATIMH010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2993413"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VLI90_07835"
     CDS             complement(<1..285)
                     /locus_tag="VLI90_07835"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HSV14154.1"
                     /translation="MMLPSHVPVLWRYRQALRSRGESRAGRLTLVAGIGYFFVWALFG
                     LVAFPIGAVLAKVEMERAILARAVPVTVGTLILIAGALQFTRWKAHHLARC"
     gene            284..574
                     /locus_tag="VLI90_07840"
     CDS             284..574
                     /locus_tag="VLI90_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14155.1"
                     /translation="MAAIITSHMARKDAAASTHVCPGMRIHIIDIVQPPGIGIPRIAG
                     MDAHEVIVSATLIANSKEEMPTNARFQVRSGGTAGPTLRDMRHLKSVGRQLG"
     gene            complement(624..1427)
                     /locus_tag="VLI90_07845"
     CDS             complement(624..1427)
                     /locus_tag="VLI90_07845"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HSV14156.1"
                     /translation="MSTSNNRLEANGNDAIAMNTPPVVSQEEWEAARQRLLAKEKAFT
                     RSRDALAAERRRMPWMAVEKKYAFDGPKGKVSLLDLFEGRRQLIVYRAFFEPGVFGWP
                     EHACRGCSLGADQVAHLAHLNARDTTLVYASRASQPDIARLKARMEWTMPWYTITDDF
                     DVDFGVDQWHGHNVFFRDGERVSRTYFVNNRGDEAMGTTWSYLDLTPLGRQETWEDSP
                     AGYPQTPPYKWWNWHDNYDSELSPNATWVQVSDAGEAAFRKRDAQEKLS"
     gene            1564..>2624
                     /locus_tag="VLI90_07850"
     CDS             1564..>2624
                     /locus_tag="VLI90_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14157.1"
                     /translation="MDSLITAAARALASGDTLGALKRVALRDDAPALALRGIAMAQLG
                     DFARGKQLLRRAARAFSSKESVPRARCIIAEAEIALASRDLGRSANALTAARVTLEKH
                     GDVLNAAHARYLQIRRLVLIGRLDEAERLLADLEPAVVPPALTAAHHLLVAGIAMRRI
                     RAATARSALADAELAAREAGIPALIGEVEHARDLLTSPAARLITRGNERPVVLEEVEA
                     LLASDAFIVDACRNVVRYGHSAISLVTRPVLFALVRSLAHAWPGDVDRGQLLARAFGA
                     RSVDESHRMRLRVEIARLRKLLRPLADVTATQRGFALAPRGAREVMILAQPVEDQNGA
                     VLALLSDGEPWSSSALAIAL"
BASE COUNT          481 a          799 c          808 g          536 t
ORIGIN      
        1 gcaacgggca aggtgatgtg ctttccagcg cgtaaactgc agggcaccgg cgatcaggat
       61 gagggtgccg acggtcaccg gaacagcacg tgctagtatg gcccgctcca tctcgacttt
      121 cgccagcacc gctccgatcg ggaacgcaac taatccgaac agagcccaga cgaaaaaata
      181 cccgataccg gcaaccagcg tgagacgtcc ggcgcgcgat tcgcccctcg agcgaagcgc
      241 ctgacggtaa cgccacagca ctggaacgtg ggacggcagc atcatggctg ccatcatcac
      301 gagccacatg gcgaggaaag atgccgcggc ctcgacccac gtttgtccgg gcatgcgcat
      361 ccacatcata gacatcgtcc agccgcccgg catcggcatc cctcgcatcg ccggcatgga
      421 tgcacacgag gtgattgtta gcgcgacgct gatcgcaaac agcaaggagg agatgccgac
      481 aaacgccagg ttccaggttc gctccggggg gacggcaggg cctaccctgc gcgacatgcg
      541 acacttgaag tcggtgggga ggcaacttgg ctgagtcatg acgccgggct ccagttccaa
      601 cagtgggttt gcagggctga aggttagctt agtttctcct gcgcgtcccg ctttcgaaat
      661 gcggcttctc cggcatcaga cacctggacc caggtcgcgt taggcgagag ctctgaatcg
      721 tagttatcgt gccagttcca ccacttgtag ggcggggtct gcgggtaacc cgcgggcgag
      781 tcctcccagg tctcctgacg gccgagcggc gtaaggtcga gatagctcca agtcgtgccc
      841 atcgcttcgt cgccacggtt gttgacgaag tatgtgcggg aaacgcgctc tccatcccgg
      901 aagaacacgt tgtgaccgtg ccattgatcc acgccgaaat ccacgtcgaa gtcgtcggtg
      961 atcgtgtacc acggcatggt ccattccatg cgtgctttca gtcgcgcaat atctggctgg
     1021 gacgcgcgtg aagcgtagac cagggtagtg tcgcgagcgt tcagatgggc caggtgggcg
     1081 acctgatcgg caccgagcga gcagcctcgg caggcgtgtt ccggccagcc gaacacgccg
     1141 ggctcgaaga atgcgcgata gacgatcaac tgtctgcggc cctcaaaaag atcgagcagg
     1201 ctaaccttgc ccttgggccc atcgaacgca tatttctttt cgaccgccat ccacggcata
     1261 cgccgacgtt ctgctgcgag cgcatcacgg gaccgggtaa atgccttctc cttcgcgagg
     1321 agtcgttggc gcgcagcctc ccactcctcc tgtgacacga ctggaggcgt gttcatcgcg
     1381 atggcgtcgt tcccatttgc ttctaatctg ttgttgcttg ttgacatgtg tatcctcgtt
     1441 ctcagttgct gagagtttgt gtctttcgtt tcgttccgca tgtaaccgca tttctcgcgg
     1501 tcatgtacaa atgttgatgc aagtctcgaa aaggccggga gtgacaagtc tggcaggatt
     1561 ccgatggatt cgctgatcac ggccgcggct cgggcgctgg cctcaggcga cactttgggg
     1621 gctttgaagc gcgttgcctt gcgtgatgat gcgcctgcac tcgcgctccg tggcatcgca
     1681 atggcgcaac tcggcgattt tgcacgagga aagcaacttc tgcgcagggc ggcacgcgcc
     1741 ttcagctcga aagaatccgt cccacgcgcg aggtgcatta tcgctgaagc cgagatcgcg
     1801 cttgcttcgc gcgacctggg tcggtctgca aacgcgctca ccgcagcacg agtgacgctc
     1861 gaaaaacatg gagacgtgtt gaacgcggcg catgcgcgtt acctgcaaat ccggcgcctc
     1921 gttctcatcg ggcgcctgga tgaagcggag cgtctgcttg ctgatctcga gcccgcggtc
     1981 gttccgcccg cattgactgc cgcacaccac ctcttagttg ctggtatcgc gatgcgccgc
     2041 atacgcgccg cgacagcgcg ttcggcgctc gccgacgcag aacttgcggc gcgggaagca
     2101 ggtatccctg ctttgatcgg ggaggttgaa catgcgcggg accttctgac atccccggca
     2161 gcgcgcctga ttacacgtgg taacgagcgc cccgtcgtgc tcgaagaagt cgaagctttg
     2221 ttggcgtcgg acgcgttcat tgtagacgca tgccgtaacg ttgtgcgcta tggtcattcg
     2281 gcgatttcgc tcgtcacgcg ccccgtgctt ttcgcactcg tacgctcgtt ggcacacgct
     2341 tggcccggcg atgttgacag aggccagctc cttgcccgtg cattcggcgc gagatccgtt
     2401 gacgagtcgc accgcatgcg gctgcgagtc gagattgcgc gacttcgcaa gctgcttcgc
     2461 ccactggccg atgtcaccgc cacccagcgt ggttttgcgc tcgcgcctcg tggcgcacgt
     2521 gaagtgatga tcctggcgca gcccgtcgaa gatcagaatg gagctgttct tgcactcctc
     2581 tcggacggtg agccgtggtc gagttcggcg ctggcgattg cgct
//
LOCUS       DATIMH010000456         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5016928, whole genome shotgun sequence.
ACCESSION   DATIMH010000456 DATIMH010000000
VERSION     DATIMH010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5016928"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VLI90_07855"
     CDS             <1..1334
                     /locus_tag="VLI90_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HSV14158.1"
                     /translation="GGSWNTAGNWSGGVPSGATARANFLSGPGLTAPATITVDGNRTV
                     GQLTFNNASAYTIAPGTGGTLTIDDSGDGNGGGGPLITVNNGNHTISAPVALTNGLTV
                     STAAGTSVTTSGGISGSGGLTKTGSGSITLGGTNNFGDITISTGTLTLASTATLNSTS
                     VNAFGGTFIANGSLNPLVTINSASPVTLGATTGASFLARPATAVNLGPTGSVTLAPAA
                     TRATRQVLIMSALNFSGSTNAWQGKLDVTNNDLIVQGGSLVQVTNQIKSGLGAANWNG
                     AAGITSANAAADAQHLTALGIIQNNDGTNHPIYGMGTIYGKFDGQNPSLNDILVKYTY
                     YGDADLNGMVDGGDYSKIDNGFNTNMGVNAGWTNGDFNYDGVVDGADYALIDNAFMNQ
                     GTALGAAVVASPTAMVAASSGFSSSSVPEPATAGIIATLAATVLSRRSRRR"
     gene            complement(1596..>2186)
                     /locus_tag="VLI90_07860"
     CDS             complement(1596..>2186)
                     /locus_tag="VLI90_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV14159.1"
                     /translation="GVVRTTQALDYATGAGRVDLSKAFDQFLGNGTHDLTGLGGGSVE
                     TVGWDYGRAALGSPSDYLIDRSLLGGSELTATLDWFADSRVDDAGFNPTYGSLDELDL
                     QVWSAIDGIASTLVAESDSAYGSSQHLSFALPSDGDYLLRVVWAQTDYNLAGDAPAAD
                     YAIAWSSTAVPEPASMTMLGIAAVSMTRLRRRRRVR"
BASE COUNT          436 a          756 c          678 g          316 t
ORIGIN      
        1 ccggcggatc gtggaacacc gccggcaact ggtctggcgg ggtgccgagc ggcgcgacgg
       61 cccgtgcgaa tttcctgtcg ggcccgggac tgacggcgcc ggcgacaatt acggttgatg
      121 gcaaccgcac cgtgggccag ctgacgttca acaacgccag cgcctatacg atcgcccccg
      181 gcaccggtgg gacgctgacc atcgacgaca gcggtgacgg caacggcggt ggtgggccgc
      241 tcatcaccgt caacaacggc aatcacacga tcagcgcgcc ggtcgcgctg accaacggcc
      301 tcaccgtcag caccgcggcc ggcacgtcgg tcaccaccag cggcggcatc agcggcagcg
      361 gtggattgac caagaccggc agcgggtcga tcacgctcgg cggaaccaac aactttggcg
      421 acatcaccat cagcactggc acgctcacgc tggcatccac cgcgacgctc aacagcacaa
      481 gcgtcaacgc gttcggcggg acgttcatcg ctaacggttc gctcaatccg ctggtgacga
      541 tcaacagcgc atcgcccgtg acgctgggcg cgacgacggg agccagcttc ctcgcgcgac
      601 cggcgacggc agtgaacctc gggccaaccg gttcggtcac gctcgccccc gcggccactc
      661 gcgcgacccg gcaggtcctc atcatgagcg cgctgaactt cagcggcagc acaaatgcct
      721 ggcagggcaa gctcgacgtc accaacaacg acttgatcgt ccagggcggc agccttgtgc
      781 aagtgacgaa ccagatcaag tccggcctcg gcgccgccaa ctggaacggc gccgccggca
      841 tcacctccgc caacgcggcg gccgatgccc agcacctgac cgcgctcggc attatccaga
      901 acaacgacgg caccaaccat ccgatctacg gcatgggcac gatctacggc aagttcgacg
      961 gccaaaaccc gtcgctgaac gacatcctgg tcaagtacac ctactatggc gacgcggacc
     1021 tgaacggcat ggtggacggc ggggactaca gcaagatcga caacggcttc aacacgaaca
     1081 tgggcgtcaa cgccggctgg accaacggcg acttcaacta cgacggcgtg gttgacgggg
     1141 cggactacgc gctgatcgac aacgcattta tgaaccaggg aacagcgctg ggcgccgcgg
     1201 tggtcgcgag tccgaccgcg atggtcgcgg catcttccgg cttcagcagc tcgagcgtcc
     1261 ccgaaccggc gacggcgggc atcatcgcaa cgctcgccgc gaccgtgcta agccgacgct
     1321 cgcgccggcg gtgattcata gtgacgacgt tcgtgttccc cgtcacagcc gcttgcggct
     1381 tagcgtctgt tttcaacaca tatcaaacgc taagccgcaa gcggcggccg ttgcgaaaaa
     1441 caaacactca cccactccgt agcaaaatcc ttcgactgtg cacgagtacg tgctgctccc
     1501 aggatgacgc gcaatcgcaa gcgcccaagc gtgcatcgat acgaacgaag ccccgcgtgt
     1561 acatgcggcg ctccatgaac gatacacctc gaacttcatc gcacgcggcg gcggcgacgc
     1621 aatcgcgtca tggaaaccgc agcgattccc aacatggtca tgctcgccgg ctccggcacg
     1681 gcggtgctcg accaggcgat cgcgtagtcg gcggcgggcg cgtcgccggc caggttgtag
     1741 tcggtctgcg cccacacaac gcgcagaagg taatcgccat cgctgggcag cgcgaacgac
     1801 aggtgctggc tcgagccgta ggcgctgtcg gattcggcga ccagcgtgct cgcgatgcca
     1861 tcgatcgcgg accagacttg caggtccaac tcgtcgagcg agccgtaggt gggattgaag
     1921 cccgcgtcat ccacgcggct gtcggcaaac cagtccagcg tcgcggtcaa ttcgctaccg
     1981 cccagcaggc tgcggtcgat caggtaatcg ctcggcgagc cgagcgccgc gcggccgtag
     2041 tcccagccca cggtctcgac gctgccgccg cccagacccg tcaaatcatg cgtgccgttg
     2101 ccgaggaatt gatcgaacgc cttggacaga tcgacgcggc ccgcgccggt ggcgtagtcc
     2161 agcgcctgcg tcgtgcgcac gacacc
//
LOCUS       DATIMH010000457         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3570852, whole genome shotgun sequence.
ACCESSION   DATIMH010000457 DATIMH010000000
VERSION     DATIMH010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3570852"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VLI90_07865"
     CDS             complement(<1..862)
                     /locus_tag="VLI90_07865"
                     /inference="COORDINATES: protein motif:HMM:NF009327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HSV14160.1"
                     /translation="MNLHQFKFVQEAVRRNLNLTEAAKALHTSQPGVSKAIIELEEEL
                     GVEIFARHGKRLKRVTEPGQHVLASIELIMREVGNLKRIGEQFSAQDSGTLSIATTHT
                     QARYVLPVPVAKLREAYPKVNVSLHQGSPDQVARMVIDEIAEIGIATESLSDYTDLVT
                     MPCYEWQHVLVLPKGHPLADRERISLEDLATEPIITYHPSFTGRTRIDHAFAQKKLTP
                     RIALEAIDSDVIKTYVRLGLGVGIVAEMAVRDDSNADLVVRPMGHIFGTNIARVAFKR
                     SAYLRNFVFKF"
     gene            complement(961..1332)
                     /locus_tag="VLI90_07870"
     CDS             complement(961..1332)
                     /locus_tag="VLI90_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HSV14161.1"
                     /translation="MIESGRAVILFAHGSRDERWRAPIEAVARRVATLDPSVSVACAY
                     LELVAPDLLTATAALVGDGARSIRVVPLFLGMGKHVREDLPRLLEGMRVQHPDVTFTL
                     ANAVGEEPDVVELLAHKALDC"
     gene            complement(1346..>2222)
                     /gene="lptG"
                     /locus_tag="VLI90_07875"
     CDS             complement(1346..>2222)
                     /gene="lptG"
                     /locus_tag="VLI90_07875"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HSV14162.1"
                     /translation="FVMARLAQSSEFTILRTSGLGPWRALRTLLLLGLGFVILTFAIG
                     DYVAPFTDRTAQLLKARFQNAYSLVGNTGAWLKERQGDASFAVNVATMERNGTLKDPR
                     IFEFDGKGFITSQVSAASARAQDGHWTLVDVERQEYQTRSADQAHIITTKIPMVEWKT
                     SLTPEMVSVALLRPERMRTLDLFDYIRHLDANGQTAQRYEIEFWRKVFYPLSCLVMIV
                     LALPFAYLHFRQAGIATYVFVGVMIGISFFLLNNVFGYLGNLGNWLPWMTAAAPGLIY
                     SVMSLAAFTWLVLRR"
BASE COUNT          427 a          668 c          707 g          420 t
ORIGIN      
        1 cgaatttgaa gacgaagttg cgcagatagg cgctgcgctt gaacgccacg cgcgcgatgt
       61 tggtgccaaa gatgtggccc atgggccgca cgacgaggtc cgcgttggaa tcatcgcgca
      121 ccgccatctc tgcaacgatt ccaacgccga ggccgagccg cacgtaggtc ttgatgacgt
      181 ccgagtcgat ggcttcgagt gcgatgcggg gcgtcagctt cttctgtgcg aaggcatggt
      241 cgatgcgcgt gcggcccgtg aaagaagggt gataggtgat gatcggttcg gtcgccaggt
      301 cctccagcga aatgcgttcc ctgtcggcca acggatgccc cttgggcagc accagtacgt
      361 gctgccattc gtagcagggc atcgtcacca ggtcggtgta gtcggagagc gactccgttg
      421 cgattccgat ctcggcgatc tcgtcgatca ccatgcgcgc cacctgatcg ggcgagcctt
      481 gatgcagact gacgttcacc ttggggtagg cctcgcggag cttggcgacc ggcacgggca
      541 gtacatagcg cgcctgcgta tgagtggtcg cgatcgacag cgtgccgctg tcctgggcgc
      601 tgaactgttc gccgatgcgc ttgaggttgc caacctcgcg catgatcagt tcgatgctgg
      661 cgagaacatg ctgccctggt tcggtgacgc gcttgaggcg cttgccgtgc cgtgcaaaga
      721 tctcgacgcc caattcttct tcgagttcga tgatggcttt ggacacgccg ggctgcgagg
      781 tatgcagcgc tttggcggct tccgtcagat tgagattgcg ccgcaccgcc tcttgaacaa
      841 acttgaactg gtgaagattc ataacgaatt ccgtcttatt taggaattta ttatgccttc
      901 gcaatctata aaaatcagcc gggaatcagt ccgaaatcag atcgaaacca gattcggaca
      961 tcagcaatcc aatgccttgt gcgcgaggag ttcgaccacg tccggctcct cgcccaccgc
     1021 gttcgcgagc gtgaaggtga cgtcggggtg ttgcacgcgc attccctcca gcaagcgggg
     1081 caggtcttcc cgcacgtgct tgcccattcc gaggaaaagc gggaccaccc gaatggaccg
     1141 ggctccgtcg cccacgagcg ccgcggtggc ggtcagcagg tcaggggcga ccagttcgag
     1201 gtaggcgcat gccacgctga ccgacgggtc aagcgtcgcg acgcgcctag ccaccgcctc
     1261 gatcggcgcc cgccagcgct catcgcgcga accgtgcgcg aagagaatga cggcgcggcc
     1321 tgactcgatc attgccgggt cgccgctagc gcctcaacac cagccacgtg aaggcggcga
     1381 gcgacatcac tgaatagatc agcccgggtg cggctgccgt catccagggc agccagttcc
     1441 cgaggttgcc caagtacccg aagacgttgt tcagcaagaa gaagctgatg ccgatcatca
     1501 cgccgacgaa cacatacgtc gcgatcccgg cctggcggaa gtgaaggtag gcaaacggca
     1561 gggccagcac gatcatcacc aggcacgaca gcggatagaa caccttgcgc cagaactcga
     1621 tttcatagcg ctgtgccgtc tgcccgtttg catcgagatg gcggatgtag tcgaacaggt
     1681 cgagcgtgcg catgcgctcc ggccggagca gggcgaccga caccatctca ggcgtgagcg
     1741 aagtcttcca ttcgaccatc gggatcttcg tggtgatgat gtgagcttgg tcggcgctgc
     1801 gcgtctggta ttcctgtcgc tccacgtcga cgagcgtcca gtgtccatct tgggcgcggg
     1861 cagaggccgc ggacacttgc gacgtgatga atcccttgcc gtcgaactcg aagatgcgcg
     1921 ggtccttgag cgtgccgttg cgctccatcg tggcgacgtt gaccgcaaac gaggcgtcgc
     1981 cttgccgctc cttgagccat gcgccggtgt tgcctacgag cgagtaggcg ttctggaagc
     2041 gcgccttgag caactgggct gtccggtcgg tgaacggtgc gacatagtcc ccgatcgcga
     2101 atgtcaggat cacgaaaccc agccccagca aaagcagcgt ccgcaacgcg cgccatgggc
     2161 cgagaccgct ggtccgcagg atggtgaatt ccgagctctg cgcaagcctg gccatgacga
     2221 ag
//
LOCUS       DATIMH010000458         6046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3857802, whole genome shotgun sequence.
ACCESSION   DATIMH010000458 DATIMH010000000
VERSION     DATIMH010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3857802"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..641)
                     /locus_tag="VLI90_07880"
     CDS             complement(165..641)
                     /locus_tag="VLI90_07880"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HSV14163.1"
                     /translation="MNLYEAAHLARELLKWHGLHDWQFAFDHARRRFGRCDYTHRRIT
                     LSRSLTFLNPVDEVRDTILHEIAHALTPGAKHGPRWRAKCRQIGARPVRCFTEAGVVT
                     PPRGPARYAIGCPACNWWAERRRRPPRGRKYVCVRCRGKIACRERVEVIEVQTFPA"
     gene            709..858
                     /locus_tag="VLI90_07885"
     CDS             709..858
                     /locus_tag="VLI90_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14164.1"
                     /translation="MNANVQQLRRIRRCHRFVTRRRPQVPLELVARLWIERYAEQWRE
                     NHRAA"
     gene            complement(886..1932)
                     /locus_tag="VLI90_07890"
     CDS             complement(886..1932)
                     /locus_tag="VLI90_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV14165.1"
                     /translation="MSDTKPKLNPQVPAFGPLPMAVIGCGRMGKLHCRFYSQMPQVKL
                     VGVYDSNPEAAADAAAEYNTKAFNSLDELLSQVAAVTIAVPTTAHPDVATRCMSRGVA
                     CLIEKPLAKDVAGARRIVDSAKSANVTVQVGHIERFNPAIRAMRQMNIKPGFIEVTRI
                     SPLTFRSIDVGVVLDMMIHDIDIVLQLAQSNVARIDATGVSITGEAEDICNARLVFEN
                     NCVANITASRLALKTERKLRVFSPDAYVSVDYQKRYGIVAHRSGNVDAIRDAVAKIKS
                     GEIQDLSDLNFADLVTIQELQIDDSDPLRAELESFINAVKNRSQPEVSADDGLAAVEL
                     ATRIVQSIATQKLA"
     gene            complement(2006..4345)
                     /gene="xdhB"
                     /locus_tag="VLI90_07895"
     CDS             complement(2006..4345)
                     /gene="xdhB"
                     /locus_tag="VLI90_07895"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HSV14166.1"
                     /translation="MPAVGQNIPHDSAAGHVTGESIFLDDVPPARGELLVDFFGSPVA
                     HGRIKRVDLAAAAAVPGVVALLTHKDIPGHNQFGPIIKDEHLLVSDLAEYIGDPIVLI
                     AAESRAAINAAKKAIKLQIEELPPVFSIDEAIAKGQFIGPQRIIERGDVNAALAGAEH
                     TLEGTLHVGGQEHFYLESHVAIAYPGEFGQMTVHSSTQHPTETQQLVAEILGVPFNKV
                     VVICKRMGGAFGGKETQAAQPAAMAAIVAAKMKRPARIVYNKDDDMRFTGKRHPFLGR
                     YKVGFDSAGHITALQLDLFSNGGCTADLSPSVLERSMLHSDNAYYLPAVRITGRVCKT
                     NLPSNTAFRGFGGPQGVANVENIIEEIAQRLGKDPLDVRQANCYGIDDGSTSLTAGRN
                     VTPYGQIVRNNTLPRLFAQLRETSEYDRRRREIAQFNDTSRTQLRGLSMTAVKFGISF
                     TKKTLNQANALVNIYTDGTVMVSTGGTEMGQGVNTRVRQIVADELGIPYDTVIVAPTS
                     TDKNNNTSPTAASSGTDLNGAAAADACCKLRSRLADFAATILNVADEGLSPSPSHVMF
                     ADGMVYDARKPDWRMTFEQLICRAYLERINLGERGFYATPGVDFNRETGKGHPFLYYT
                     NGVAASEVLIDRFTGELKVTRVDLLMDAGQCINPGIDRGQVTGGFIQGMGWVTTEELK
                     YSQHGELLSHSPTTYKIPNISDVPETFNVAFLDNPDNVVSIRRSKALGEPPLLLGISV
                     WTAVKDALSSAAGAPVQMQLPATGETILMAMERTKRVMT"
     gene            complement(4354..5874)
                     /locus_tag="VLI90_07900"
     CDS             complement(4354..5874)
                     /locus_tag="VLI90_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HSV14167.1"
                     /translation="MRNFVLIYVNGQRHELRGPRLFQSLSDFLRYELQLTGTKVVCAE
                     GDCGSCTVFLGRPRGGAIEYRTVCSCIQYLYQLDGCHIVTVEGLKYDGQMNPIQSAMV
                     RCQGTQCGFCTPGFVVSMCALFEGDGPITEHRLRRGLIGNLCRCTGYEPILKAGLAID
                     ASQVKRLDELYPPAPLLLALAPASNDAVLVQDSDRTFFKPTSIADAARFKQEHADCVI
                     ISGGTDLGVQMNKGVRALRTVMSTAALEPLRELSVSNEQIVAGASVSIAELENAAAKA
                     LPEYAALLGWFGSPPIKNAGTVGGNIANASPIGDSMPAMFVLNAEIELTGVNDSRRVN
                     INSFYTGYKKSVATADELITRVFIPLPKVGEVFKLYKVSKRKDLDISSFTAAVWMSVS
                     DHTIGEVRIAYGGVAATIVRLPKTEAALNGQRISEALFDDAADVAVGEIKPISDVRGS
                     ADYRLRLAANVLRKFFIETFGGGSNGDGRGPNGDGNGHPPTHAKSRLSALDVVVRK"
BASE COUNT         1061 a         2035 c         1800 g         1150 t
ORIGIN      
        1 tcggcggtac tccgtcgacg aaagcaatcg agtcagcgac aacgctctga caactgtcgc
       61 tagcgcgatg gctgtctcga tggcgtacca tcgccatgtg tgagtacacg caccacgggc
      121 cggatgcgcc gtgccacgaa acgcgtgcgc ctgcggggct accgctacgc ggggaaagtt
      181 tgcacttcga tgacttcgac gcgttctcga cacgcgatct ttccccggca ccgcacgcac
      241 acatacttcc gcccgcgcgg cgggcgccgc cggcgctcag cccaccaatt acacgccgga
      301 catccgatcg catatcgcgc cggcccccgc ggcggcgtca caacacctgc ttccgtaaaa
      361 caccgcaccg gccgcgcgcc gatctggcgg cacttcgccc gccagcgcgg cccatgtttc
      421 gcccccggcg tcagcgcgtg cgcgatctcg tgcagaatcg tgtcgcgcac ttcatcaacc
      481 ggattcaaaa atgtgagtga gcgcgacagc gtgatccgcc gatgcgtata atcacaccgg
      541 ccgaaccgcc ggcgggcgtg gtcgaacgcg aactgccaat cgtgcagccc gtgccatttc
      601 agcagctcac gcgcgagatg ggcagcctcg tacaggttca cgtcttatcc accatccctg
      661 gcacgcgact cgcatatcac attgttgatg atgaatggag tcgcaatcat gaacgccaac
      721 gtgcaacagc ttcgccggat tcgtcgctgt caccgcttcg tcactcggcg gcgcccgcag
      781 gtgccgctgg aacttgtcgc ccggctgtgg atcgaacgct acgccgagca atggcgagaa
      841 aaccatcggg cggcgtgacg cccgcgcatt gatgcgcgcc gtgcttcacg ccagcttctg
      901 cgtcgcgatc gactgcacga tccgcgtcgc cagttccacc gccgccagcc catcgtccgc
      961 cgacacctcc ggctgcgacc gattcttcac ggcgttgatg aacgactcca gctccgcccg
     1021 cagcggatcg ctgtcgtcga tctgcagttc ctgaatcgtc accaggtccg caaaattcaa
     1081 gtcagacagg tcctggatct cccccgactt gatcttcgcc accgcatcgc gaatcgcgtc
     1141 cacgtttcca ctgcggtgcg cgacgatgcc gtagcgcttc tggtaatcca ccgagacgta
     1201 tgcatcgggc gaaaagacgc gcagcttgcg ctccgtcttc agcgccagcc gagacgcggt
     1261 gatgttcgcg acgcagttgt tttcaaaaac cagccgggca ttgcagatgt cctccgcttc
     1321 gccggtgatg ctcacgccgg tcgcgtcgat gcgggcaacg tttgactgcg ccagctgcag
     1381 cacgatgtcg atgtcgtgga tcatcatgtc cagcacgacg ccgacgtcga tgctgcggaa
     1441 cgtcagcggg ctgatgcggg tgacctcgat gaagcccggt ttgatgttca tctgccgcat
     1501 ggcgcggatg gcgggattga agcgctcgat gtggccgacc tgcaccgtga cgttggccga
     1561 cttggcgcta tcgacgatcc gccgcgcgcc ggcgacgtct ttggcgagcg gcttttcgat
     1621 caggcacgcg acgccgcgcg acatgcagcg cgtcgccacg tccgggtggg cagtggtggg
     1681 caccgcgatc gtcacggccg ccacttgcga cagcagttcg tccaggctgt tgaacgcctt
     1741 ggtgttgtat tccgccgctg cgtcggccgc cgcctcgggg ttggaatcgt aaacgccgac
     1801 cagcttcacc tgcggcatct ggctatagaa gcggcaatgc agcttcccca tccgcccgca
     1861 cccgatcacc gccataggca acggcccaaa cgccggcacc tgcgggttca attttggttt
     1921 ggtgtcactc acgcaaggca tgttacacgg gacgcggcgg cgtgtcgcat ggtgggatgt
     1981 agtgcgtaat gcgtacgaat atcggtcacg tcatcacgcg ttttgttctc tccatcgcca
     2041 tcaggatcgt ctcgcccgtc gcggggagct gcatttgcac cggcgcgcct gccgccgacg
     2101 acagcgcatc cttcaccgcc gtccacacgg agatgcccag cagcagcggc ggctcgccca
     2161 gcgctttgct tcggcggatg ctgaccacgt tgtcggggtt gtctagaaaa gccacgttga
     2221 aggtctccgg cacatcgctg atgttcggga tcttgtacgt cgtcggcgaa tgcgacagca
     2281 attcgccgtg ctgcgaatat ttcagctctt cggtcgtcac ccaacccatg ccttggatga
     2341 acccgccagt gacctgcccg cggtcgatgc cggggttgat gcactggccg gcgtccatca
     2401 gcaggtccac gcgcgtcacc ttcaactcgc cggtgaagcg gtcgatcagc acctcgctgg
     2461 cggcgacccc gttggtgtaa tagaggaacg gatggccctt gccggtttca cgattgaagt
     2521 ccacgccggg cgtggcgtag aagccgcgct cgccgaggtt gatgcgttcg agatacgccc
     2581 ggcagatcag ctgctcgaac gtcatccgcc agtccggctt gcgcgcgtcg tacaccattc
     2641 catcggcgaa catgacgtgc gatggcgacg ggctcaaacc ttcatccgcc acattcaata
     2701 tcgtcgccgc gaagtccgcc aatcgcgacc gcagcttgca gcaagcgtca gcggccgcgg
     2761 cgccgttcaa gtcggtgccg ctcgatgcgg ccgttggtga ggtgttgttg ttcttgtccg
     2821 tgctggtcgg ggcgacgatc accgtgtcgt acggaatgcc caattcatcg gccacgatct
     2881 gccgcacgcg ggtgttcaca ccctggccca tctcggtgcc gccggtgctc accatgacgg
     2941 tgccgtcggt ataaatgttg actagcgcgt tcgcctggtt cagcgtcttc ttcgtgaacg
     3001 agatgccgaa cttcaccgcc gtcatcgaca gcccgcgcag ctgcgtccgg ctggtgtcgt
     3061 tgaactgcgc gatctcgcgc cgccggcgat cgtactccga cgtctcccgc agctgcgcga
     3121 acaatcgcgg caacgtgttg ttccgcacga tctggccgta cggcgtcacg tttcgacctg
     3181 cggtgagcga agtcgaaccg tcgtcgatcc cgtaacaatt cgcctgccgc acatccagcg
     3241 gatccttccc caggcgctgc gcgatctcct cgatgatgtt ctcaacattc gccacgccct
     3301 gcggcccgcc aaacccgcgg aacgcggtgt tgctcggcag gttcgtcttg cacacgcgcc
     3361 ccgtgatgcg cacggccggc aggtaatacg cgttgtccga atgcagcatc gaacgttcga
     3421 gcacgctcgg cgacaggtcg gccgtgcacc cgccgttcga gaacaaatcc agctgcagcg
     3481 ccgtgatgtg accggccgaa tcgaagccaa ctttgtatcg gcccaggaac ggatggcgct
     3541 ttccggtgaa gcgcatgtca tcgtctttgt tgtagacgat ccgcgccggc cgcttcatct
     3601 tcgccgcgac gatggcggcc atcgcggccg gttgggcggc ctgcgtttct tttccaccga
     3661 atgcgccgcc catgcgtttg cagatcacca cgaccttgtt gaacggcacg ccgaggatct
     3721 cagccaccag ctgctgcgtt tccgtcggat gctgcgtcga cgaatgcacc gtcatctgcc
     3781 cgaattcgcc cggatacgcg atggcgacgt gcgattcgag ataaaaatgt tcctgcccgc
     3841 cgacgtgcag ggtaccttcg agcgtgtgct ccgcgccggc caacgccgcg ttcacatcgc
     3901 cgcgttcgat gatgcgctgc ggcccgatga attgtccctt ggcgatcgct tcatcgatcg
     3961 aaaacaccgg cggcagctct tcgatctgaa gcttgattgc cttcttcgcg gcgttgatcg
     4021 ccgcccggct ttccgcggcg atcagcacga tcggatcgcc gatgtactcc gccagatcac
     4081 tgaccagcag atgttcatcc ttgatgatcg gcccgaactg attgtgcccc gggatgtcct
     4141 tgtgcgtcag cagcgcgacc acgcccggca ccgccgcggc ggcggcgaga tcgacgcgct
     4201 tgatccgacc atgcgcgacc gggctgccaa agaaatcgac tagcaactcc ccgcgcgccg
     4261 gcggcacgtc gtcgagaaag atcgactccc ccgtcacatg cccggcggcc gaatcgtgcg
     4321 gaatgttttg accgacagct ggcattgcaa tgctcacttc cgaacgacta catccagtgc
     4381 tgacagccgc gacttggcat gggtcggcgg atgaccgttg ccgtcaccgt tcggaccgcg
     4441 tccgtcgccg ttgctgccgc cgccgaacgt ctcgatgaag aactttcgca agacgtttgc
     4501 tgccaatcgc aggcggtagt cggctgaccc gcgcacgtcg ctgatcggct tgatttcacc
     4561 gactgcgaca tccgccgcgt catcaaagag cgcttcggaa atgcgctgcc cgttcagggc
     4621 cgcttcagtc ttcggcagcc gtacgatcgt cgccgccaca ccgccatagg cgatgcgcac
     4681 ctcgccgatc gtgtgatcgg agactgacat ccacaccgcc gcggtgaagc tggaaatgtc
     4741 caggtctttc cgcttcgaca ccttgtacag cttgaacacc tcgcccacct tcggcagcgg
     4801 gatgaagacg cgcgtaatca gctcatccgc cgtcgcgacg ctcttcttgt agcccgtgta
     4861 gaacgagttg atgttgacac gtcgcgagtc gttcacgccg gtcagttcga tctccgcatt
     4921 cagcacgaac atcgccggca tcgaatcgcc gatcggcgac gcgttggcga tgttcccgcc
     4981 aacggtcccc gcgttcttga tcggcggcga tccgaaccag cccagcaacg ccgcgtattc
     5041 cggcagcgcc ttggccgcgg cgttttcaag ttccgcgatc gacacgctcg cgccggcgac
     5101 gatctgctcg ttcgacacgc tcaactcgcg cagcggctcc agcgcggcgg tgctcatcac
     5161 cgtgcgcagc gcgcgaacgc ctttgttcat ctgcacgccc agatcggtcc cgccggagat
     5221 gatcacgcag tccgcgtgct cctgcttgaa ccgcgcggcg tcggcaatac tcgtgggctt
     5281 gaagaacgtc cgatccgaat cctgaaccag caccgcatca tttgaagctg gcgccagcgc
     5341 gaggagcagc ggcgccggcg ggtacagttc atcgagccgt ttcacctgcg aagcgtcgat
     5401 cgccaatccc gctttcaaaa tcggctcata ccccgtgcac cggcacaggt tcccgatcaa
     5461 cccgcggcga aggcggtgtt cggtgatcgg gccgtcgccc tcgaacagcg cgcacatcga
     5521 cacgacaaac cccggcgtgc agaagccgca ctgcgtgcct tggcagcgca ccatcgccga
     5581 ctgaatcggg ttcatctgcc cgtcgtactt caggccctcg acggtgacga tgtggcagcc
     5641 gtcgagctgg tagaggtact gaatgcacga gcagacggta cggtattcga tcgccccgcc
     5701 gcgtggcctg ccgaggaaga cggtgcacga cccgcagtcg ccttcagcgc agacgacctt
     5761 cgtgccggtc aattgcagtt cgtagcggag gaagtcggag agcgactgaa agagccgcgg
     5821 cccgcgcagc tcgtgccgct gaccgttgac atagatcagg acgaaattac gcatcactta
     5881 tcaatcccga cgggcgccgc gcgagcggat cgttcgcgcg gatcagtata ccatggcggg
     5941 gtggtttgcc gaacatcgaa cgcgccgcag gatacgaaag cgttacgacc gccgcttgcg
     6001 gcttcgcaca ccacgccacc gggattgcga agccgcaagc ggcagc
//
LOCUS       DATIMH010000459         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5017267, whole genome shotgun sequence.
ACCESSION   DATIMH010000459 DATIMH010000000
VERSION     DATIMH010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5017267"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VLI90_07905"
     CDS             <1..182
                     /locus_tag="VLI90_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSV14168.1"
                     /translation="GNIPGAVAGGLLLGLTEVLVAGYLPGGSQYRDGVAFVILIAILL
                     VKPSGLLGKNVIEKV"
     gene            220..>1560
                     /locus_tag="VLI90_07910"
     CDS             220..>1560
                     /locus_tag="VLI90_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSV14169.1"
                     /translation="MSPWIVTLLTNVWVPIALLLIALVGRRYENADWPGAYIMHIVML
                     VGINIILAVSLQLINGVSGQFSLGHAGFMALGAYLAGYAIKTFAAVSADADDPASDYF
                     NPAATLLFFIALLIAVALAAAVIFGIFLLIRQSRRLHPSLPGVLLVALLAWFLADIAK
                     GMEPGDAPKYCIFTRLIGGDHALFDSIIAHGTSRAGKWSDALPVGARKPTTLLISLIG
                     GGAMAAVAGLIVGLPTLRLRGDYLAIATLGFGEIIRIAIVNSPPLGGATGLQAPTYAI
                     KPDIEEGIRGYYIAPWIYGAVLLTILLIWRLARSTRGRAIRAVREDEIAAAATGISTT
                     HQKVTAFVFGAFFAGVAGALYVNLDGYLNTASFGFMRSIEIVVMVTLGGLGSIWGAAI
                     AAVILTILPEVLRNVSPQVSEWRMVIYSLLLIGLMLMRSGWWRRAPLIRAIPFIG"
BASE COUNT          233 a          495 c          500 g          332 t
ORIGIN      
        1 tcgggaatat ccccggcgcg gtggccggtg ggttgctgct gggactgact gaggttcttg
       61 tggccggtta cctgccgggc ggcagccagt accgcgatgg ggttgcgttc gttatcctca
      121 tcgccatcct gctggtgaag ccttcgggcc tgctcgggaa gaatgtcatc gagaaagtct
      181 gacacctccg ccaccgctct tccgctcaac gcgggcggca tgtcgccgtg gatcgtcacg
      241 ctcctcacaa acgtctgggt accaatcgcg ctgctgttga tcgcgcttgt tggaagacgt
      301 tacgaaaacg ccgactggcc cggcgcctac atcatgcaca tcgtgatgct cgtcgggatc
      361 aacatcatcc tggcggtcag cctgcagctg atcaacggcg tgagcgggca gttttcgctc
      421 ggccacgccg gcttcatggc cctcggcgcg tatcttgccg gttatgcgat caagacattc
      481 gccgccgtca gcgctgatgc ggatgacccg gccagcgatt acttcaaccc cgcggcaacg
      541 ctgctgttct tcatcgcgct gctgatcgcc gtggcgctgg cggcggcggt gatcttcggc
      601 atcttcctgc tgatccgcca atcgcgccgg ttgcacccgt cgctgccggg cgtgctgctg
      661 gtggcgcttc tcgcgtggtt cctggccgac attgccaagg ggatggagcc gggcgacgcc
      721 ccgaagtatt gcatcttcac gcggctgatc ggcggggatc atgcgctgtt cgattccatc
      781 attgcgcacg gaacttcgag ggccggcaaa tggtccgatg cactgcccgt cggcgcgcga
      841 aagccgacga cgctgctcat ctccctgatc ggcggcggcg cgatggctgc cgttgccggg
      901 ttgatcgtcg gcttgccgac gctgcgcttg cgcggcgact atctcgccat cgcgacgctc
      961 ggctttggtg aaatcatccg catcgcgatc gtgaattcgc cgccgcttgg tggcgccact
     1021 ggactacaag cgccgacata cgccatcaaa cccgatatcg aagaaggcat ccgcggctat
     1081 tatatcgcgc cgtggatcta cggcgcggtg ctgctgacga tcctgctcat ctggcggctg
     1141 gcgcgatcaa cgcgcggccg ggcgatccgc gcggtgcgcg aagacgagat cgccgccgcc
     1201 gcgacgggaa tcagcacgac gcatcagaaa gtgacggcat tcgtcttcgg cgcgttcttc
     1261 gccggcgtcg ccggggcgct ttatgtgaac ctcgacggtt acctcaacac cgcgtcgttc
     1321 ggcttcatgc gatcgatcga aatcgtcgtg atggtcacgc tcggtggctt gggcagcatc
     1381 tggggcgcgg ccatcgccgc cgtcatcctg acgatcctgc cggaagtgct gcgcaatgtt
     1441 tcacctcaag tttcggagtg gcgcatggtg atctattcgc tgctgctgat cggtttgatg
     1501 ctcatgcgct ccggctggtg gcggcgggcg ccgctgattc gcgcgattcc gttcatcggc
//
LOCUS       DATIMH010000460         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3858073, whole genome shotgun sequence.
ACCESSION   DATIMH010000460 DATIMH010000000
VERSION     DATIMH010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3858073"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1714)
                     /locus_tag="VLI90_07915"
     CDS             complement(305..1714)
                     /locus_tag="VLI90_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSV14170.1"
                     /translation="MYKLHLIKKYLLKRRIAWVALLAVMLCTAMVLVVISVMGGWLRM
                     FQDSFHGITGDVVVEARSLAGFPYYQEIIDRAKKEVPGVETAVPVISTGGMVNINNIQ
                     QPVQVLGFTNEIGTVIDWPTSLHSQYETRADKPKTPEPDFSLLPNVDYAQLAGRAPDH
                     GKRPGMIVSAALIGIHGGKDAATQERNEETRDIMRNARVSLMVVPMKPGQGLTDIAPV
                     QQGFWIVDDSRTRLWQVDYNTVYVPFDVIQRDLHMEAFDNEPARASEIRVKAKPGADL
                     VAVRDAVRQVVDAVYAEHPAEDTAYYPVRVMTWEESQAKFIDAVKHEVVLTTMLFGII
                     SMVAVLLIFCIFYMIVVEKTKDIGIIKSVGATGQGIMSLFLGYGLAIGVVGAGLGFLF
                     AYLVVRYINELHAWMGRAMGIKIWDPETYQFETIPNKMNPTTVFWILVVAVLSALLGA
                     LVPAIRAARMNPVEALRYE"
     gene            complement(1858..>2153)
                     /locus_tag="VLI90_07920"
     CDS             complement(1858..>2153)
                     /locus_tag="VLI90_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HSV14171.1"
                     /translation="ADDPFFADVYELAINGQEISPGYSELNDPDVQAANFAHQVGDEA
                     EKQKTDEDFLNALRYGMPPAGGMGLGIDRLVMMLTGAESIRDVILFPLMKPQT"
BASE COUNT          384 a          697 c          647 g          425 t
ORIGIN      
        1 gccttcgatc gcgacgaact cgcccgtctt caacgacatg tcgcagtgct tcagcacggt
       61 caattcgcta tcgcccatgc ggaacgactt gcggatgccg cgcacgcgca ggatgacgtc
      121 gctggagaca gcgcgagagg atgcgagggg ttcaacggct tgcgacatca caatactcaa
      181 tcatctgtgg aacccgacca cgccgggttc agagcgtact cgcgacgacc cggatgcttg
      241 ggcgcgtgag agaagaagca tccgggtcgt cggaatacct ccgaacccgg cgtgggtctg
      301 gcgcctactc gtaccgcagc gcttccaccg ggttcatgcg cgccgcgcgg atcgcgggca
      361 ccagcgcgcc cagcagtgcc gacagcaccg cgacgaccag gatccaaaac accgtcgtcg
      421 ggttcatctt attggggatc gtttcgaact gatacgtttc agggtcccag atcttgatgc
      481 ccatcgcccg gcccatccac gcgtgcagct cgttgatgta gcgcacgacc aggtaggcga
      541 ataggaagcc cagcccggcg ccgacaacgc cgatcgccaa gccatatcca aggaacagac
      601 tcatgatccc ttgcccggtc gcgccgacgc tcttgatgat gccgatgtcc ttcgtcttct
      661 ccaccacgat catgtagaag atgcagaaga tcagcagcac cgcgaccatg ctgatgatgc
      721 cgaagagcat cgtcgtcagc acgacttcgt gcttcaccgc gtcgatgaac ttcgcctggc
      781 tttcttccca cgtcatcacg cgcacggggt agtacgccgt gtcctccgcg gggtgctcgg
      841 cgtacaccgc gtccaccacc tgccgcaccg cgtctctgac ggcgaccaga tcggcgccgg
      901 gctttgcctt cacgcgaatc tcgctcgcgc gggccggctc gttgtcgaac gcctccatgt
      961 gcaggtcgcg ttggatcacg tcgaagggga cgtataccgt gttgtaatcc acctgccaca
     1021 gccgcgtacg gctgtcatcg acgatccaga acccctgctg caccggcgcg atgtccgtca
     1081 ggccctgccc cggcttcatg gggacgacca tcagcgacac gcgcgcgttg cgcatgatgt
     1141 cacgcgtctc ttcgttgcgc tcctgcgtgg ccgcgtcttt cccgccgtgg atgccgatca
     1201 gcgctgcgct gacgatcatg cccgggcgct tgccgtggtc cggcgctcgg ccggcgagct
     1261 gcgcataatc gacgttcggc aacagcgaga aatccggttc cggcgtcttc ggtttgtcgg
     1321 cccgcgtttc atattgcgaa tgcaggctgg tcggccaatc gatgaccgtg ccgatttcgt
     1381 tggtgaaccc cagcacctgc accggctgct ggatgttgtt gatgttgacc atcccgccgg
     1441 tgctgatgac cggcacggcg gtctcgacgc cggggacctc cttctttgcg cggtcgatga
     1501 tctcctggta atagggaaag cccgcgagcg agcgggcttc aacgacgaca tcgccggtga
     1561 tgccgtggaa cgaatcctga aacatccgca accacccgcc catgacgctg atgacgacca
     1621 gcaccatggc ggtgcagagc atgacggcaa gcagcgccac ccaagcgatg cggcgtttga
     1681 ggagatattt tttgatgagg tggagtttgt acacgtgtga gcggtcaggg gtcaggggtc
     1741 aggagcatgt cgaatccgtt atagccgcag gcgacagcca gcggggtctt tcgataatca
     1801 acccggttga cttctcaacc ccccgctggc tgtcgcccgc ggctatcagg gcgcccttta
     1861 cgtctgcggt ttcatcagcg ggaacaggat cacatcgcga atgctttcgg cgccggtcag
     1921 catcatcacc agacgatcga tgcccagtcc catcccgccg gctgggggca ttccgtaccg
     1981 cagcgcgttg aggaaatcct catccgtctt ctgcttctcc gcttcgtctc cgacttggtg
     2041 ggcgaagttc gccgcctgca cgtcggggtc gttcagctca ctgtagccgg gcgaaatctc
     2101 ctggccgttg atcgcgagtt cgtacacgtc ggcgaagaat gggtcgtccg cgt
//
LOCUS       DATIMH010000461         6238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4147946, whole genome shotgun sequence.
ACCESSION   DATIMH010000461 DATIMH010000000
VERSION     DATIMH010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4147946"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VLI90_07925"
     CDS             complement(<1..164)
                     /locus_tag="VLI90_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSV14172.1"
                     /translation="MSRDLTKIRNIGIAAHIDAGKTTVSERVLFYTGRIHRRGEVHHG
                     NTTLDWMPQE"
     gene            complement(292..1215)
                     /locus_tag="VLI90_07930"
     CDS             complement(292..1215)
                     /locus_tag="VLI90_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSV14173.1"
                     /translation="MLALVSSLGLPVLAQSAPLLNPVVILVLCVIAGLGTVMLLPGHR
                     EMAFRQIGGVILLAAGLIFFALMVRYFVGLTKTSSVAVYFWIFATIAVFGALRVITHP
                     RPVYSALYFVLTVFASAGLFILLEAEFMAAALVLIYAGAILVTYVFVIMLASQAVSPE
                     SQTPAQQLAEYDNVSREPLAASAIGFALMGVLLYVIFDRAGDLVTPSGARPLPDAVPV
                     VGATQELGMFLFRNQLVQLELAGLLLTVAMVGAIVIARRQVLVPDIEQPIGLKSKPDV
                     VIGPATPVDDNPHSIPVYGTENPRQKAYPET"
     gene            complement(1639..2226)
                     /locus_tag="VLI90_07935"
     CDS             complement(1639..2226)
                     /locus_tag="VLI90_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HSV14174.1"
                     /translation="MSLLTGKRIAILVENGFEQVEMTEPRQALNDAGATTVLVSPVQG
                     KVKGWKHTEWGDEFAVEMQLSAADADTFDALLLPGGVMNPDKLRINPDAKRFVRSFAE
                     AGKPIAAICHGPWTLIEIGAVRGRTMTSWPSLQTDLRNAGANWVDREVVVDKGLVTSR
                     KPDDIPAFNRKMIEEFTEGVHHTRQHSTRPNPTAM"
     gene            complement(2342..3835)
                     /locus_tag="VLI90_07940"
     CDS             complement(2342..3835)
                     /locus_tag="VLI90_07940"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HSV14175.1"
                     /translation="MPVNPCIPAPCVVIGEDGLSVTLKVSHSDAEASRPTPANLLSYL
                     AGLGVTVADPKVIERLAGRDGRIKIEKDIVLVQGRRPVPERPGRVEMLVHAGQVNEAI
                     DGPPVNPNTSVVSHYERASYAAAVAGQAIATITPVTPGVDGRDVFGAPIPHARVRTPV
                     ELGRNVELDPDGVTVRATAPGRVCHAARTLWVETTVKIAGDVNFGCGNIDVAGDVLVG
                     GSVLDMFKVRGANIRVNGAIEAAEVTATENLLVAGGILGKGKGRCQAGGDVACKYASN
                     ATLIAGGDIKVRGEISHTRIIARGRLAAETAALTSGHITANGGITCRSLGSVIEAQTV
                     VEVGIDAMLRTAGPQRLAEINTIKARATQRLDTAAQLLRFQKSLTAQQKEEAADLLRQ
                     ASALETEAADLLQPLLAAAQDSRQKLLAEVFVEDMLHAGVTVRFANCETTIRMDWQGP
                     LRLSVQLDDGEAQVVLTDISSNHVQVLPGKPWHDAAYDTFLRAIASA"
     gene            4102..4923
                     /locus_tag="VLI90_07945"
     CDS             4102..4923
                     /locus_tag="VLI90_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV14176.1"
                     /translation="MPQRRFTFVHLMVSLAIVMFIAAYFASFLSVHSNRGESSSRVKC
                     ASNLRQIGQAMLLYANDNHQHYPRVVYTPSPLVVPTWGTGAAAADPFKPDGTSPNDVS
                     AALFLLLRTQDITPEVFVCPNSNAEKDDFGGGTNTAANRSNFTDWKKNLSYSLHNPYP
                     NEGAIPKDHEARGWTTSMPADFAVAADLNPGTAGGRDVVHVTIGSSASQMRKANSTNH
                     DQDGQNVLYGDGHVEFQNNPFCGTKRDNIYTAGDGQVIASPVNEEDSVLLPAQDW"
     gene            5236..6093
                     /locus_tag="VLI90_07950"
     CDS             5236..6093
                     /locus_tag="VLI90_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14177.1"
                     /translation="MTYCLGIVTKYGLIMASDSRTNAGYDQVNTVRKMHTFVTPGERV
                     FVTLASGSLSCTQSVLTLLKRDFDQGKGLATAPSMYDAARIIGDKVRVVGDMDRDALA
                     KDEYKFNVHMILGGQVKGEAPQLFMIYPQGNPLQATEESPYLQIGETKYGRPILDRGI
                     RFEKTSLEEAAKYALLSMDSTMKSNVTVGPPIDLLAYSTDELEITRTRRFMTDDPDLN
                     KIRVRWEQALRQAIARLPELRFRVHAPSGTSEKMDEETIQLVDRAGDPAGAEAQPQAS
                     QQSRAPDSK"
BASE COUNT         1159 a         2017 c         1946 g         1116 t
ORIGIN      
        1 tgctcctgcg gcatccagtc cagcgtggtg ttgccgtggt ggacctcgcc cctgcggtgg
       61 atgcggcccg tgtagaagag cacgcgctcg gagacggtgg tcttgcccgc gtcgatgtgg
      121 gcggcgatgc cgatgttgcg aatcttcgtc aagtcgcgag acataactgg tcctttgtag
      181 cacgggctcc gagccttcgg ctctctcgcc cgtgtttcgc cgctatagcg gctcttggtt
      241 ttccataatc acgcgcgacg ctgcgctcaa atcacgggcg acacgcccgt gctacgtttc
      301 cgggtacgcc ttctgccgcg ggttctccgt cccgtacact gggatgctgt gcgggttgtc
      361 gtccaccgga gtggccgggc cgatcactac atccggcttg ctcttcaaac cgatcggttg
      421 ctcgatgtcg ggcaccagca cttgccgccg ggcgatcacg atcgcgccga ccatcgccac
      481 cgtcagcaac aatcccgcta attcaagctg taccagctgg ttgcggaaca gaaacatgcc
      541 cagttcctgc gtggcgccaa ccaccggcac tgcgtcgggc aatggccggg cgccgctcgg
      601 tgtcactaag tcgccggcgc gatcaaaaat cacgtacagc agcacgccca tcagcgcgaa
      661 accgatcgcg ctggcggcca gcggttcgcg gctcacgttg tcgtactccg ccagttgctg
      721 cgctggcgtc tgcgattcag ggctcaccgc ctgcgacgcc agcatgatca cgaatacata
      781 agtgacgagg atcgccccgg catagatcaa cacgagcgcg gcggccatga actccgcctc
      841 cagcaatatg aataggccag ccgacgcgaa aaccgtcaat acaaagtaga gagccgagta
      901 aacgggccga ggatgggtga tgacgcgaag cgcgccgaag acggcgatcg tcgcaaagat
      961 ccagaagtag acggccaccg aggacgtctt ggtcaagccg acgaagtatc gcaccatcaa
     1021 cgcgaagaag atcagccccg ccgccagcag gatgacgccg ccgatctggc gaaacgccat
     1081 ctcgcgatgg ccgggcagca gcatgaccgt accgagcccc gcgatgacgc acaggaccag
     1141 gatgacgacg gggttcagca gtggagccga ctgggcaagc accggcaggc cgagcgatga
     1201 aaccaaggca agcatgagcg cgtccaaccg ccgcaaagcg aagcaggcga gccccggcga
     1261 cgcgccggcc tccgcccgtt ttcaagtggc ggaaggataa gagtggccta aaagctttgc
     1321 aagacgtgtg tgacgctttt cacgaagagc gggaagcgag gacaagacgt gcagcatgtg
     1381 cccccacgcc gggttcggag gtactccgac gacccggatg gattgggatg gggtgcaggg
     1441 ggtgctggag tcagttgtgt ggcgcgtata ccacaactga caactgacca ctgacaactg
     1501 accactgaca actgaccact gaccactgac aactgaccac tgacaactga caactgacaa
     1561 cgaagcatcc gggtcgtcgc gaatacgctc cgaacccggc gtggggcttc accacgtctt
     1621 tctgatttcc agggcatctc acatcgccgt cgggttcggc cgcgttgaat gctgccgcgt
     1681 gtggtgcacg ccttcggtga actcttcgat catcttgcga ttgaacgcgg gaatgtcatc
     1741 tggcttgcgg ctggtgacca ggcccttgtc caccaccacc tcgcggtcca cccaattcgc
     1801 gccggcattg cgaagatcgg tctgcaatga cggccaactt gtcatcgtcc ggccgcggac
     1861 cgcgccgatc tcgatcagcg tccaaggccc gtggcagatc gccgcgatcg gtttccccgc
     1921 ttcggcaaag ctgcgcacga aacgctttgc gtcgggattg attcgcagtt tgtccgggtt
     1981 catcacccca cccggcagca gcagggcgtc gaacgtgtcg gcgtcggcgg cggacaactg
     2041 catctccacc gcgaattcat cgccccattc cgtgtgcttc caacctttga cttttccctg
     2101 caccggcgaa accagaaccg tcgtcgcccc cgcgtcattg agcgcctgcc gcggctcggt
     2161 catctccact tgctcaaagc cattctccac cagaatggcg atgcgcttgc ccgtgagcaa
     2221 actcatggca atcacctttc cagcccgcat cctgcgagct tttatgctgc gctgcagatt
     2281 gcaaaaggcg gaccaccgtc gcgaccgctt cggatacgga tttgcctcag gtggaaaacg
     2341 actaggcgct ggcgatggcg cggagaaacg tgtcgtacgc cgcgtcgtgc cacggcttgc
     2401 cgggcaggac ctgaacgtga tttgacgaga tgtcggttag aacgacctgc gcctcgccgt
     2461 cgtccaactg cacgctcagg cgcagcgggc cttgccaatc catgcggatc gtcgtctcgc
     2521 agttggcgaa ccgcacggtc acgccggcgt gcagcatgtc ttcgacgaag acttcggcca
     2581 gcagcttctg ccggctgtcc tgcgccgcgg ccagcagcgg ttgcagcagg tcggccgctt
     2641 cggtctccag cgcgctggct tggcgcagca ggtcggccgc ctcctccttt tgctgagccg
     2701 tcaggctttt ctgaaagcga agcagctgtg ccgcggtgtc gagccgctgg gtggcgcggg
     2761 ccttgatcgt gttgatctcc gccagccgct gcggcccggc ggtgcgcagc atcgcgtcga
     2821 tgcccacctc gaccaccgtc tgggcttcga tgaccgaacc gagcgaccgg caggtgatgc
     2881 cgccgttggc ggtgatgtga ccggacgtca gcgccgccgt ttccgcggcc aatcggccgc
     2941 gcgcgatgat gcgcgtgtgc gaaatttcgc cgcgcacttt gatatcgccc ccggcgatca
     3001 acgtggcatt gctcgcgtat ttgcaggcga catcaccacc ggcctggcag cgccccttgc
     3061 ctttgccgag gatgccaccg gcgacgagca ggttctccgt cgcggtgacc tcggcggctt
     3121 cgatcgcgcc gttcacgcgg atgttcgccc cgcgcacctt gaacatatcc agcacgctgc
     3181 cgccgaccag cacgtcgccg gccacgtcga tgttcccgca gccgaagttc acgtcacccg
     3241 cgatcttcac ggtcgtctca acccatagcg ttcgcgcggc gtggcagacc cggcccggcg
     3301 cggtggcgcg cacggtgacg ccatcgggat ccagctcgac gttgcgcccc aattccaccg
     3361 gcgtgcgcac gcgcgcgtgc gggatcggcg cgccgaagac atcgcgacca tcgacgccgg
     3421 gcgtgaccgg cgtgatcgtc gcgatcgcct gcccggcaac cgcggcggcg tatgacgcgc
     3481 gctcgtaatg gctgaccacg gaagtgttcg ggttcaccgg cgggccatcg atcgcttcgt
     3541 tcacctgacc ggcgtgaacg agcatctcaa cgcgcccggg gcgctccggc accggccggc
     3601 ggccttgcac gagcacgatg tctttttcga tcttgatgcg accgtctcgt ccggcgaggc
     3661 gctcgatcac tttcggatcc gccaccgtca cgccgagccc ggcgagatag gaaagcaagt
     3721 tggctggtgt ggggcgcgac gcctcggcgt cgctgtggct caccttcagc gtgacggaca
     3781 gaccgtcttc gccgatcacc acgcaggggg cggggatgca cgggttcact ggcatgtaca
     3841 actgctcccc gcagcgaatg ggcgtcacgc aagcctgcac cgcgcgccga catgagggcg
     3901 gcctgcgcgg gcgtgttcgc gtgttatcga ctacggcggt tctccaattc agctatcggg
     3961 aaatacgcag cgctcattca tattcgcgcg agccggttcc gttttttagt cacgtcccgt
     4021 aactgttgcg cagcgtgcgc atgttcttgc ttgtaccgcg tgcgcgttca atccttcggc
     4081 gcctgactgg gagaacccat catgccgcag cgtcgattca cctttgtgca cctgatggtt
     4141 tcgctggcga tcgtgatgtt catcgccgcg tatttcgcgt cgttcctgtc ggttcattcg
     4201 aaccgcgggg agagttccag ccgcgtaaaa tgcgccagca atctccgcca gatcgggcag
     4261 gcgatgttgt tgtacgccaa cgacaatcac cagcattatc cgcgtgtcgt ttacacgcca
     4321 tcgccgctgg tggtgccgac gtggggcacc ggtgcggcgg cagcggatcc gttcaagccg
     4381 gacgggacga gccccaacga tgttagcgct gcgctgttcc tgctgctgcg cacgcaggac
     4441 atcacgccgg aggtgttcgt ctgtccgaac agcaacgccg aaaaggacga cttcggcggc
     4501 ggcacgaaca ccgcggcgaa ccgctcgaac ttcaccgact ggaagaagaa cctcagctac
     4561 agcctgcaca atccgtatcc caatgagggc gcgattccca aggaccacga ggcgcgaggg
     4621 tggaccactt caatgcccgc ggacttcgcc gttgccgcgg acctgaaccc cggcaccgcc
     4681 ggtgggcgcg atgtggttca cgtgacgatt ggatcatcgg ccagccagat gcgcaaggcc
     4741 aacagcacga accacgatca ggacggccag aacgtcctgt atggcgacgg gcacgtcgag
     4801 ttccagaaca accccttctg cggcacgaag cgcgacaaca tctacaccgc tggtgatgga
     4861 caagtgatcg catcgccggt gaatgaagaa gacagcgtcc tgctgccggc gcaggattgg
     4921 taggggcgtt aacgcagcgg tcatcctgag cggagcacgt acccgtgcgc agtcgaagga
     4981 tcttgcatcg aaggacgatg aacgctcgct tcatcaggcg ttcatcgact gctcggcaag
     5041 atccttcgac tccggcgagt acgcctgcgc tcaggatgac actatataaa aagcccatgg
     5101 gtttttttcg gggattctcg ccgcgccatc gcgccagtgt ttgggcgcac cgttcgcgtt
     5161 tgttaggatg tgccggccgg ccccgcccgc gctggggccg ttcgttttgc gcgaaaggcg
     5221 cgtcggtcgg cacacatgac ctattgtctg ggcatcgtca ccaaatacgg cttgattatg
     5281 gcgtccgact cgcgcaccaa cgcggggtat gaccaggtca acaccgttcg caagatgcac
     5341 acgttcgtca cgcccggcga acgcgtgttc gtcacgctcg ccagcggcag cttgtcctgc
     5401 acgcaatccg ttttgacgtt gctcaagcgc gacttcgacc agggcaaagg cctggccacc
     5461 gcgccgagca tgtacgatgc cgcccggatt atcggtgaca aggtccgcgt cgtcggcgac
     5521 atggaccgcg atgcgctggc caaggacgaa tacaaattca acgtccacat gatcctcggc
     5581 gggcaagtca aaggcgaagc cccgcagcta ttcatgatct acccgcaggg caacccgctg
     5641 caggcgaccg aagagtcgcc gtatctgcag atcggcgaaa cgaaatacgg ccggccgatc
     5701 ctcgaccgcg gaatccggtt cgagaaaacg tcgctggaag aggcggccaa gtacgcgctg
     5761 ctgtcgatgg acagcacgat gaagagcaac gtgacggtcg gcccaccgat cgacctgctg
     5821 gcgtattcga cggacgagct ggagatcacg cgcacgcggc gattcatgac cgacgacccg
     5881 gacctgaaca agatccgcgt gcgctgggaa caggcgctgc gccaggcgat cgcgcgcttg
     5941 cccgaattaa gattccgcgt gcacgcgcca tcgggcacgt cggagaagat ggacgaggag
     6001 acgatccagc tggtggaccg agccggcgac cccgcggggg cggaagcgca gccgcaggcg
     6061 tcccagcaat cgcgggcgcc ggattcgaag tgaaggcggc ttgtcatcct ccagcgcagg
     6121 cggtactccg ccggtatcga aggatctggc cgagaagcgg gagaaccgtc acttgcgcga
     6181 gtggcgcagc ggtcgcttgc ggtttcgcag gccagtgatc gcggtttgtc cgtctgcg
//
LOCUS       DATIMH010000462         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3858510, whole genome shotgun sequence.
ACCESSION   DATIMH010000462 DATIMH010000000
VERSION     DATIMH010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3858510"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VLI90_07955"
     CDS             <1..525
                     /locus_tag="VLI90_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HSV14178.1"
                     /translation="LRAARDAGIKVEKETIDKAIKYVRGCQNPDGGFSYMMRMGGGGG
                     SGFARSAAGVAALYYAGVFEGNDLERGLKYIKQFMPGKGAARVGPNGEPMDMEGHYYY
                     GNYYAVQAMFLAGGDYWAEWYPAVREQLISRQSPAGNWSGDFSDDYATSMALIILQMP
                     NRYLPVYSGKGPGS"
     gene            607..1881
                     /locus_tag="VLI90_07960"
     CDS             607..1881
                     /locus_tag="VLI90_07960"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HSV14179.1"
                     /translation="MRRFTRIFVPTFIAMFVCALARADDWSITTADFQTHQAAALRGL
                     GADGVRFVAAGTDAQEQTIPLDQFVSLQRAASDAAPTATTKFTLFLAGGDRLTGEPVR
                     VANEQVTWHSPALGEITLPLQRLVSLVRGGDGEPAAPAEPASQDVARLANGDSVAGVA
                     TDITADHVTIKTDAGAADVPLGSVTQLTFATVASHASTNAAGAFQVKLIDGSAVTVAD
                     ASIDQQSLKIALGPKSSAQHQIDVPLAQISAIEQLNGPVTWLSSLTPIERVQTPYFGG
                     SPPWPARNDAAVDGGPLHAAGQSFTRGIGVHSYSRLTYDIAAGNWPAFRTQYAIDTGT
                     GDAPRQLADVTVRIKLDDRVVHEQAHIRAGELSPVFNVDLSGAKRLTLEVDYGDAGDT
                     QDHFDWIEPALLRHAAVVAPPPSTEPAPNGMK"
BASE COUNT          389 a          654 c          703 g          361 t
ORIGIN      
        1 cttcgcgccg cgcgcgacgc gggcattaag gtggagaagg agacgatcga caaggcgatc
       61 aagtacgtcc gcggctgcca gaaccccgac ggtgggttca gctacatgat gcgcatgggc
      121 ggcggcgggg ggagcgggtt cgcccgcagc gccgcgggtg tcgccgcgct ttattacgcg
      181 ggcgtctttg aaggcaatga tctggagcgc ggactgaaat acatcaagca gttcatgccg
      241 ggcaaagggg cagcgcgcgt cggtcccaac ggtgaaccaa tggacatgga aggccattat
      301 tattacggca actattatgc ggtgcaggcg atgttcctgg ccggcggtga ttactgggcc
      361 gagtggtacc ccgcggtgcg cgagcagctg atcagccggc aaagccccgc cggcaactgg
      421 tccggcgact tcagcgacga ttacgcgacg agcatggcac tcattatcct gcagatgccg
      481 aatcggtatc tgccggtgta ttccggcaag gggccgggga gctagagatc cgatgtagcg
      541 agggcgccag agcccgtgac tgacgactct cgacgcgcgc tctggcgccc gtgctacaac
      601 atcatcatgc gtcgcttcac ccgcatcttt gttccgactt tcatcgcgat gttcgtttgc
      661 gcgctcgcgc gtgcagatga ctggtccatc accaccgcgg attttcaaac gcaccaggcg
      721 gcggcacttc gcggccttgg cgcagatggc gttcgatttg tggcagcagg aacggatgcc
      781 caggagcaga cgatcccgct cgatcaattc gtctcgctgc agcgcgcggc atcggacgcg
      841 gcgccaacgg cgacgacgaa gttcacactg ttccttgccg gcggagaccg actcaccggc
      901 gagcctgtgc gcgtcgcgaa cgagcaggtg acttggcaca gccccgcgct gggcgagatc
      961 acgctgccgc tgcagcggct ggtgtcgctc gtccgcggcg gcgacggtga accagcagcg
     1021 ccggccgagc cggcgtcgca ggacgtggcg cgtctcgcca acggcgacag tgtcgccggc
     1081 gtggcgacgg acatcaccgc cgatcatgtc acgatcaaga ccgacgccgg cgcggccgac
     1141 gtgccgcttg gctcggtaac gcagctgacc tttgcgaccg tcgcgtcgca tgcttcgaca
     1201 aacgcggcgg gagcgttcca ggtcaagctg atcgatggct ccgccgtcac cgtcgccgac
     1261 gcgtcgatcg atcaacagtc gctgaagatc gcgctggggc cgaagtcgtc ggcgcagcac
     1321 cagattgatg tgccgctggc tcagatcagc gcgatcgagc agctgaacgg gccggtcacg
     1381 tggctgtcgt cgctgacgcc gatcgagcgc gtgcagacgc catatttcgg cgggtccccg
     1441 ccgtggccgg cacgcaacga tgccgcggtc gatggtgggc cgctgcacgc ggctgggcaa
     1501 agtttcacgc gcgggatcgg cgtgcacagt tactcgcggc tgacgtacga catcgccgcc
     1561 ggcaattggc ccgcgttccg cacgcagtac gcaattgaca ccggcaccgg agacgccccg
     1621 cgccagttgg cggacgtgac ggttcgcata aagctggatg atcgagtcgt tcacgaacag
     1681 gcgcacattc gcgccggcga gttatcgccg gtcttcaacg tggatctgag cggggcgaaa
     1741 cggctgacgc tggaagtaga ctacggcgac gcgggggata cgcaggatca tttcgactgg
     1801 atcgaaccgg cgctgttgcg ccacgcggcg gtagtggcgc caccgcccag caccgagccg
     1861 gcgccaaacg gcatgaagtg atatgtaccc ctctaacttc tagccaccct tcgagctaat
     1921 cattctcgcc taataatggc gctgtctctg tcatcccgaa cgcagcatgt actcatgcgc
     1981 ggtcgaggga tctagtcgag aagtaagtga cgcgtgcatc cgcgccggag ccccgcatgt
     2041 acatgctgag gacccgacac ttttgcagga atggtgggaa accaatcgcg ccgggttcgg
     2101 aggtact
//
LOCUS       DATIMH010000463         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_107836, whole genome shotgun sequence.
ACCESSION   DATIMH010000463 DATIMH010000000
VERSION     DATIMH010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107836"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VLI90_07965"
     CDS             <1..284
                     /locus_tag="VLI90_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14180.1"
                     /translation="PDELYHFYRKELPLAGGISNVDMTRSWAAQYPSLLIDYIRVSPS
                     GPMTPIVITTTTLGDRFHVGLTHRVGLIRPERAAALAQQFLARLGSLHA"
     gene            343..852
                     /locus_tag="VLI90_07970"
     CDS             343..852
                     /locus_tag="VLI90_07970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSV14181.1"
                     /translation="MAKQYSAPPMTIDPNKKYTATIDTTAGTMVAELFPKDAPQHVNS
                     FVFLAREGFYNGVIFHRVIPGFMIQGGDPTGTGTGGPGYKVKAEFNSRKHTRGVLSAA
                     RSSDPNSAGSQFFVMHDTAPHLDGQYTAFGQVTSGLEVIDKIVNAPKDRNDRPKNPTA
                     INSITIEEA"
     gene            complement(992..2191)
                     /locus_tag="VLI90_07975"
     CDS             complement(992..2191)
                     /locus_tag="VLI90_07975"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSV14182.1"
                     /translation="MRWFRFAALACLCIPVVVMADEQPPPIRLEPVVKNLDAATYLIS
                     DGTKRLFVCEQIGRVRLIEDGKLVKQPALDISKEVWFQGECGFLSIAFHPDYQKNGLV
                     YAYYINNAAAPGPSPGGKKVKGGGEYWAIVSEFKADPATGKFDPSSERQLLKFDEHPY
                     GNHKGGQLQFGPDGYLYVGAGDGGLHDDPRNNAQNLASPLGKIHRIDVNKRDGDKPYG
                     IPPDNPFVGKPGAMPEIFCYGMRNPWRFSFDVPTGDIYCADIGQDKYEEIDLIIKGGN
                     YGWRGREGLHPNPTLKRTYSPGVQEVPGAIDPILEYPHSQNNKSITGGYVYRGKAIPG
                     LVGWYIYGDYSSGRIWALKQKDGKVLANVELLHSRTQPSSFGQDAEGELYDVDYDGAI
                     WKIVGGS"
     gene            complement(2230..2586)
                     /locus_tag="VLI90_07980"
     CDS             complement(2230..2586)
                     /locus_tag="VLI90_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HSV14183.1"
                     /translation="MGDGSLQELTQLALALRDERDWAQFHNPKDVALSMSLEVAELVE
                     IMQWKNGAELQEHLAAQRDHLGQELSDVLYWVLVLAHDQQIDLATAFKAKLQQNAAKY
                     PVEKARGIARKYHDLG"
BASE COUNT          493 a          860 c          772 g          538 t
ORIGIN      
        1 gccccgatga gctgtaccac ttttatcgca aggaactccc gctggccggc ggcatttcca
       61 acgtggacat gacccgcagc tgggcggcgc aatatccgtc gctgctgatc gattacatcc
      121 gcgtttcacc gagcgggccg atgacgccga tcgtcatcac cacaacgacg ctcggcgacc
      181 gctttcacgt cggcctcacg catcgcgtcg ggctcattcg acccgagcgc gccgcggcgc
      241 tcgcccagca attcctcgct cgcctcggct cgcttcatgc ttgacacccc ggcaaaaaac
      301 tgaaccctag cccgtcccaa tccaaccaaa ggagccgcga ccatggccaa gcagtattcc
      361 gccccgccga tgacgatcga tccgaacaag aaatacaccg cgaccatcga caccaccgcc
      421 ggcacgatgg tggccgagct gttccccaag gacgccccgc agcacgtgaa cagctttgtc
      481 tttctcgcgc gcgagggctt ctacaacggg gtgatcttcc accgcgtgat ccccgggttc
      541 atgatccagg gcggcgaccc caccggcacc ggcacgggcg gccctggtta taaggtgaag
      601 gccgagttca acagccgcaa gcacacccgc ggcgtgctgt cggcggcgcg cagcagcgat
      661 cccaattcgg ctggctccca gttcttcgtc atgcacgaca ccgccccgca cctggacggg
      721 cagtataccg cattcgggca ggtgactagc gggctcgagg tgatcgacaa gatcgtgaac
      781 gcgccgaagg atcggaatga tcggccgaag aatccgactg cgattaatag tattacgatt
      841 gaagaagctt gaagcgcgtc ttgccccctc gcccacgtac tcgtgggaga gggctggggt
      901 gagggtgatt tcgagcacta accgctatcg acattctaaa tcgccctcac cctaaccctc
      961 tcccatgtgt acatgggcga ggggaccatg actacgaccc gcccacgatt ttccaaatcg
     1021 ccccgtcgta atcgacgtcg tacaactccc cctccgcgtc ctggccaaaa ctcgacggct
     1081 gcgtccggct gtgtagcagc tcgacgttcg ccagcacttt gccgtccttt tgtttcagcg
     1141 cccagattcg gccgctgctg tagtcgccgt agatgtacca gccgaccagg cccgggatgg
     1201 ccttgccgcg gtagacgtag ccgccggtga tgctcttgtt gttctggctg tgcggatatt
     1261 ccaggatcgg gtcgatcgcg ccgggaactt cctgcacgcc gggcgaatag gtgcgcttga
     1321 gcgtgggatt cgggtgcagg ccctcccggc cgcgccagcc gtagttaccg cctttgatga
     1381 tcaggtcgat ctcttcgtat ttgtcctggc cgatgtcggc gcaataaatg tcaccggtcg
     1441 gcacatcgaa tgaaaagcgc cacgggtttc gcatgccgta gcagaagatc tcaggcatcg
     1501 cgccgggctt gcccacgaac ggattatctg gcggaatgcc gtatggctta tcgccgtcgc
     1561 gcttgttcac atcgatgcgg tggatttttc ccagcggcga cgcgaggttc tgcgcgttgt
     1621 tgcgcgggtc atcgtgcaac ccgccgtcgc cggcgccgac gtacagataa ccgtcggggc
     1681 cgaactgcag ttgcccaccc ttgtggttcc cgtacgggtg ctcgtcgaac ttcagcagtt
     1741 ggcgttcgct cgatggatcg aattttccgg tagccggatc ggctttgaat tcagagacga
     1801 tcgcccaata ttcgccgccg cccttgacct ttttgccgcc gggcgacggg ccgggcgctg
     1861 cggcgttgtt gatgtagtac gcgtacacca agccgttctt ttgataatcc ggatggaacg
     1921 cgatgctgag gaatccgcac tcgccctgga accagacttc cttgctgatg tccagcgcgg
     1981 gctgtttgac cagcttgccg tcttcgatga ggcgcacgcg gccgatttgc tcgcagacga
     2041 acagccgctt ggtgccatcg ctgatcagat aggtggcggc gtcgagattc ttgacgaccg
     2101 gctcaagccg aatcggcggc ggttgctcgt ccgccatgac gacgacggga atgcacaagc
     2161 acgcgagagc ggcaaatcga aaccatctca tgttgctgga taatacgggc ggcaggcgaa
     2221 gccgttgaat cagcccaaat cgtggtattt gcgggcgatg ccgcgggcct tctccaccgg
     2281 atacttcgcc gcgttctgct gcagcttcgc tttgaacgcg gttgccaagt cgatctgctg
     2341 atcgtgggcc agcacgagca cccaatacag cacatcggac aattcctggc ccaggtgatc
     2401 gcgctgagcc gccaggtgct cctgcagttc cgcgccattc ttccactgca tgatctcgac
     2461 cagctcggct acctcgagcg acatcgacag cgcgacgtcc ttggggttat ggaactgcgc
     2521 ccagtcgcgt tcatcgcgaa gcgcgagcgc gagctgcgtc agttcctgca gtgaaccatc
     2581 acccatctca tcctccagcg gttcttctat caatcgtagt ccgccatttg tagcagcgac
     2641 gcctggcgga ccaacggctc aat
//
LOCUS       DATIMH010000464        14394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_687605, whole genome shotgun sequence.
ACCESSION   DATIMH010000464 DATIMH010000000
VERSION     DATIMH010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 14394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14394
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687605"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VLI90_07985"
     CDS             <1..1265
                     /locus_tag="VLI90_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSV14184.1"
                     /translation="TFLAAGLKARAVSFKTWDTAVGELDNLLEIRNHDYVKARTYGFE
                     LTAIMLGAVAGFVLLITRTINKPLKLISRSLNEGASATAQASTQVASSAQSLASGATE
                     QAASLEETSAALEEMSSMTKKSEETAQKASSYSAEAHRAALRGNEAMQKMSGAIQQIQ
                     KSAGETAKIIKVIDEIAFQTNLLALNAAVEAARAGEAGRGFAVVADEVRSLAMRSAEA
                     AKNTAALIEESVQNSRNGVAITTEVGKNLEEITTASTQVNSLVGEIAAACTEQARGIE
                     QVNIAVSQMDQVTQQNASNAEESASASEELAAQAERLGGVVRRLIDMVGRSSATAATA
                     GDTNADNATAEAAEETAVAAPAKKSAKARTASAGRGKGGPKANAAATTPRSFREVAKQ
                     QIPFDDDDESAPAAGDPASDFGDFSKAA"
     gene            1407..2354
                     /locus_tag="VLI90_07990"
     CDS             1407..2354
                     /locus_tag="VLI90_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HSV14185.1"
                     /translation="MPKAVASSQIVIASPTITQAEFDRLREMVYRHCGIHLKEGKQEL
                     VRARVARRLRTGAFPSLNAYLDFVVRDKTGREMTDLIDSISTNLTSFFRESGHFTYLR
                     EQFLPQLLERKRRAGCNTIRAWSAGCSTGEEPYSLAMLLHEIVVAQGGWDVKILATDV
                     SRRVLRIARGGLYPENKISRVPEHMRAKYFRRAQREAEEGFFVEPRIRQLIQFNYLNL
                     MEKWPFSGQMDFIFCRNVMIYFDQPTQERLIQRFYTHLQDGGVLMTGHSESLSGMNHN
                     YHYIQPTVYGKRVMVAPLTPAPAAATAGLTPLAQQGRAA"
     gene            2351..3514
                     /locus_tag="VLI90_07995"
     CDS             2351..3514
                     /locus_tag="VLI90_07995"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HSV14186.1"
                     /translation="MNAAAGSTHVPQRRIRVLIVDDSAIVRQVLQRLLGSDPQIEVVA
                     TAHDAYVAREKIVRYEPDVVTLDVNMPRMDGITFLRKLMAHHPVPVIVVSGQTSDGTA
                     TALEALCAGAVDLVSKPAGEADLAGLGSVLIDKIKSAARARVKLAPAAVVSSVQADAC
                     KMPRIAPAAAANQPASGPSDPLIFAIGASTGGVQALTEVLTAFPEDAPPTLIVQHMPA
                     KFTGPFAQRLNKLCRVEIREAANGDRLSPGTVLIAPGGFHMTLRRRAGGFFGVEVRDG
                     PELFHQKPSVEVLFNSVAKAAGANAVGAILTGMGADGAQGLLRMRQAGAFTLAQDEAS
                     CVVFGMPCEAIKCGAAHQVVPLTGVADAMMAAAQRHERGGSAARVPINQRMSA"
     gene            3557..4102
                     /locus_tag="VLI90_08000"
     CDS             3557..4102
                     /locus_tag="VLI90_08000"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HSV14187.1"
                     /translation="MQNQATMQPPALAAPANGKPAGVAAAVTTTLDPKLIAPFVSSIR
                     AVFSTMVGVETTVQRPYLKNDETHSFAVSSIIGFSGQVVGSVTLSFQEETAKKLVAKF
                     AGAELGIHDPDFADAVGELANMVAGAAKKDLGGTASITVPSVVIGSGHNIARLRDVPC
                     VVVPCTSPLGDFTVEVSIKRV"
     gene            4148..4537
                     /locus_tag="VLI90_08005"
     CDS             4148..4537
                     /locus_tag="VLI90_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV14188.1"
                     /translation="MKILLVDDSKTMRNIQKKTLERLGTVEFVEAGDGLEALTAIAAH
                     PGIDLMLVDWNMPNMDGHTLVCRVREKDKTTPIIMCTTEAEKTRVIDALKAGVNNYIV
                     KPFTPDAMSDKVQQTLAKAKGAVAAAA"
     gene            4606..5787
                     /locus_tag="VLI90_08010"
     CDS             4606..5787
                     /locus_tag="VLI90_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HSV14189.1"
                     /translation="MQTDTENQVHHAIELLVEHEAGEEHSHYDRSNFLFVEAGQPIAK
                     LAADAEHLIGLSLKNVNRTPEGMFVATVAGCLKADHAHLWVEPGLRVAGNVDFSSGNV
                     EYKGDVCVIGNVVDLFKVNATGAVTIAGTIEAAEVSAGTDLTAKGGIAGREKGICRAN
                     GKLTAKYISGATVEVGGDVQVLDEVSHARLVCGGRLKVTHRTIVASHITANGGVECAN
                     LGAPGAGAKTIIEVGVDAQVRKLAQVTFPAVQRMRHKAAQVRLSIEPLMRDQKKLTVA
                     QRERATELLYQADEIEQEANDKDKALRDAFERAQAKRSTEIVVGGKLFAGTIVRLGEL
                     EQTIDNTADGPLTITLRDHEGTQQIALVQTHGGSTRFIGGRPVRDPEMDAMRQQFSKA
                     A"
     gene            5851..6336
                     /locus_tag="VLI90_08015"
     CDS             5851..6336
                     /locus_tag="VLI90_08015"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HSV14190.1"
                     /translation="MDVNLINPFIIAARGVFDTMVRLPLMSGKPHVRASSQAVNRTFK
                     VSVQIRFHGAAEGIAVLHMSEAVALAMATAFSGEPYLSINADVHDALGEVANLIIGSA
                     KEHLGPDLHISVPKVYDASEVHCPTGSPVVVIPFDTTAGRFVLEVSLQSNPAAKAVRA
                     A"
     gene            complement(6656..14305)
                     /locus_tag="VLI90_08020"
     CDS             complement(6656..14305)
                     /locus_tag="VLI90_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSV14191.1"
                     /translation="MRYLGPTSSHGNVAELEFDGVMSAPPPSGNALTGAVIGTPGSYN
                     NSGNTIAKAFDGSLSDYFDAQQPDGAWAGMDLGAPAQITQIKYAPRSSFAGRMVGGIF
                     QGSNTADFSSGVVNLYTVTTSPAVGSFTTVPVTDPGTYRYVRYLGPTSSHGNVAEIEF
                     DGVAGTTPAQTGPKLIGTAIGSAGSGSSTGPAKAFDFSFNTAFDASVADFAWTGIDTG
                     GENVAAQIKFAPRSGQAARMVGGIFQGSDTADFSSGVVNLYTVPSAPTVGAWTTVTTG
                     VSTPFRYLRYIGPTGSHGDVAEIEFDNQVTGPTPNPSIRSVTPINGATAVARNAFVSC
                     DLNLPNLASGIDVSTLNANTVQLFRTFDHQLVDAAVNTDGAGSVVVLQPFTPLDASTT
                     YTFVVNAGLKDALGATFQPFSITFTTGSAPITTDPNIAFEKVTLPTAVNQMFTGVTMG
                     PDDALYASTLNGHIFRFPVNTDGTLGNPFDIQTVINDPSNAGGRLITGISFDPTSTAS
                     NMTIWVSSGNGIIYGAPDFTGKMSTISGSGTSWGNYKDMVIGLPRSSQDHLNNQPVFG
                     PDGALYWGQGSNSAMGAPDPVWGLRQEHLLNAAILRLDVTAIRSYVATNGKALNVQTD
                     SLPAGQTAYNPFAAGAALTLYATGVRNDYDLVFDSNGHLYAPTNGSAAGGNVPATPAG
                     VTPSGPALNNVAVAEDDWLFDIQKGGYYGHPNPSRNEFIMNGGNPTTPLGPNFPIQNA
                     YPVGTNPDPNYRGVALDLGVHYSPDGALEYHGSAFNGALDHAILATRYSGGKDLLLIR
                     TGANGQVTQTETGIAGFTGFNNPIDVVEDPHTGFLYVAELGGMKLTLLRPINSGAQVS
                     VSASTMYFNDPVGGSAGPAQSLTITNTGSAPLALPSDGLTVTGADGSLFPIVSKPSLP
                     ATIPAFGSITITLAFNPASTSALGLHTATLEIKTNDPNTPLLDVNLRALATAGLGGNK
                     EPSLQRILDLYQIPIQTGTADPSVTAFTTPPGTPNDEVVMPRLVKAGTGPVTVTPLAV
                     FSASITPSAGFGYYTAGTPDSKTEVFNVGTADAQSVHVTPIGATSFDPGSSVFGLYSS
                     FDAFPTSVGTPRIAYSEDEFNTYDTSNNNRKVRFYPLKNSDGSIVPNAFIFATEDYGI
                     APSYDSNDVVGIIRNVQAAPVGPELGLQNQDGAPFVDRLALNDIQNSNATHPNVFHNK
                     ATVRVWNTGDQAMMVNSITSSNAGFTVGTFPSSIAANSFADVVVTYLPTHTGNNASDT
                     GTLTISTNAINGATRTVQLDGFWQLWGNSNPTTGAYTEPSVSTIINLLGYKTTILNSG
                     QNMNQGGKIATVGEEVLSNYWVRADTNLPVSARQLASFHTQGNQDTLYWFSKGSPTTY
                     NAVANAVGADGQSFLPQSNDTTHAAAFGTFSTNNTFGFNIDKQESSDDSLNNKPTTTD
                     QGHHLRFWPARDRSGNLIPNTWLMSMDYAGINYDYNDNVFLISNMRPAPPAAPTTPVA
                     TGSGSGISLTWTAVSAPLLAGYNVYRSTSSTGTFVKINSSVVATASDNDATAAAGSTY
                     YYKITATDTWGCESAQSAVASATRPADSVAPAAPAGLTATGSSSGVTLSWTANIEADL
                     AGYNVYRSTSATGTFFKLNTSGLITGTNFVDTTAPAGQASFYQVTAVDASSNESTAAT
                     ANATRLVDTTPPAVPTGFSANGSTANITLSWSANTDSDLAGYNLYRSNTSGGALTKLN
                     STPLTALSFVDTAAQLSLPSYYQLRAVDTSGNESAGTAANAVHKDSTLPAAPANLNSS
                     GSSTAITLTWAANTETDLAGYRVYRATSATGTYTLLNTTQLLSSAGYVDSAALSGVAS
                     FYHVTAVDFSGNESSASSTSATRTGGGDITPPAQPTGFIANGTTSGVNLSWTANTESD
                     LAGYNVYSSLSPTGTFTIVNKSGLITTTSYADTLATASVTTYYHLTAVDKSNNESSFA
                     TTQALRPDTTAPSTPKNFTATGSIGGIALSWTANTETDLAGYNVYRAASAAGPFTTKI
                     NTSGLVTANSFNDTSATVGATNYYQIKAVDTSGNSSTPTSANAFRPSPNDITPPAQPS
                     NLAASGAAGGITLTWSANTEPDLAGYNVYSGPSASGPFTKLNTAGLLKVLTYNDTAAV
                     QGATTFYRLSAVDLSNNESTFATASALRPDATAPATPANFTATGSTAGIALAWNANTE
                     SDLAGYNLYSSTSATGTFTLVNRGGSITGTSYNDTAAPSGAVTFYHLTAVDTSNNEST
                     TPANANALRPNPTDTTPPATPTNFGAVGSAGGVSLTWSANTEPDLAGYNLYRAPTAGG
                     TFVKLNTTGFLTGVTYNDTTAPAGQMSFYQLTAVDTSANESTPPATASASVPVQSTFT
                     SADIGNPPIAGSTTTITPGSDYDMAASGANINGTADQFRYVYKQVSGDFDVKVQIASL
                     SNGDGTTSGYARAGIMVRTDLSAGSANLYTFTLAGPFGFITTDRPTAGATTTQSAAIS
                     NNPAPQKWVRMVRVGDTYTTYASIDGTTWNPIGPAVTVPLGQTVYLGMAATSHQTDHL
                     VIAKFRSFTGA"
BASE COUNT         2478 a         4482 c         4862 g         2572 t
ORIGIN      
        1 ccacgtttct ggcggccggc ctgaaggccc gggcggtctc gttcaagacg tgggacactg
       61 ccgtgggtga actcgacaac ctgctcgaga tccgcaacca cgattatgtc aaagcccgca
      121 cgtacggctt tgagttgacc gcgatcatgc tcggcgccgt tgccgggttc gtgctgctga
      181 tcacgcggac gatcaacaag ccgttgaagc tgatctcgcg ttcgctgaac gaaggcgcgt
      241 ccgccacggc gcaggcgtcc acgcaagtcg ccagcagcgc gcagtcgctg gccagcgggg
      301 ccaccgagca ggcggcatcg ctggaagaga ccagcgctgc attggaagag atgagcagca
      361 tgaccaagaa gagcgaagag accgcacaga aggccagcag ttattcggct gaggcgcatc
      421 gcgcagccct ccgcggcaac gaggccatgc agaagatgag cggcgccatc cagcagatcc
      481 agaagtctgc cggcgagact gccaagatca tcaaggtgat cgatgaaatc gcgttccaga
      541 caaacctgct ggcgttgaac gccgcggtgg aggccgcccg cgcgggcgaa gcgggccgtg
      601 ggttcgcggt cgttgccgat gaagtgcgca gcctcgccat gcgttcggcc gaagcggcga
      661 agaacaccgc cgcgctgatc gaagagagcg tgcagaacag ccgcaatggt gtggcgatca
      721 ccaccgaggt gggcaagaac ctggaggaga tcaccaccgc cagcacgcag gtgaattcgc
      781 tggttggcga gattgccgcg gcttgcaccg agcaggcccg gggcatcgaa caggtgaaca
      841 tcgctgtgtc gcagatggac caggtgacgc agcagaacgc gagcaatgcc gaggagtcgg
      901 cgtcggcgtc ggaagaattg gcggcccagg ccgagcggtt aggtggggtg gttcgccgct
      961 tgatcgacat ggtcggccgc agcagcgcca ccgccgcaac ggccggcgat acgaacgccg
     1021 acaacgcgac tgcggaagcg gcggaggaaa ctgccgtggc agcgccggcg aagaagtcgg
     1081 cgaaggcccg caccgcctcg gccgggcgcg gcaagggcgg ccccaaggcc aacgcggcgg
     1141 cgacaactcc ccgcagcttc cgcgaagtgg ccaagcagca gattccgttc gatgacgacg
     1201 acgagagcgc cccggccgcc ggcgaccccg ccagcgactt cggcgatttc tcgaaggccg
     1261 cgtgatcgcg cgccgtcagg tatcgaggag ccggtaacgg ttgagtcgcg gtggcggtgc
     1321 gcaggggaat tccggcgcgc cgccacccga ataaccgcgg ctcatggatt gtcaggatga
     1381 ttcccctact cgagaccgag tcacctatgc ccaaggcagt tgcttcatca cagatcgtta
     1441 tcgcctcacc gaccatcacg caggcggagt tcgaccgcct gcgcgagatg gtgtaccgcc
     1501 actgcgggat ccacctgaag gaaggcaagc aggagctggt tcgcgcccgc gtcgcccggc
     1561 gattgcgcac gggggcgttc ccgtcgctca acgcgtacct ggatttcgtc gttcgcgaca
     1621 agaccggccg cgagatgacg gacctgatcg attcgatcag cacgaacctg acgtcgttct
     1681 ttcgcgagag cgggcacttc acgtatctgc gcgagcagtt cctgccgcag ctgttggagc
     1741 gcaaacgccg cgccggctgc aacacgatcc gcgcatggag cgcgggctgt tccaccggcg
     1801 aggagccata ttcgctcgcc atgctgctgc acgaaattgt cgtcgcgcag ggcggctggg
     1861 atgtgaagat cctggcgacg gacgtgtcgc gacgcgtgct gcgcatcgcg cgcggcggat
     1921 tgtacccgga gaacaagatc agccgcgtgc cagagcacat gcgcgctaag tatttccgcc
     1981 gcgctcagcg cgaggcggaa gagggtttct tcgtcgagcc gcgcatccgg cagctcatcc
     2041 agttcaacta cctgaacctc atggaaaagt ggccgttcag cgggcagatg gatttcatct
     2101 tctgccgcaa tgtcatgatc tatttcgatc aaccgacgca ggagcggctc atccagcggt
     2161 tctacacgca tctgcaggat ggcggcgtgc tgatgaccgg gcattccgaa tcgctttcgg
     2221 ggatgaacca caactaccac tacattcagc ccaccgttta cggcaagcgc gtgatggtcg
     2281 ctccgctgac gcccgcgccc gcggcggcaa cagcagggtt gacaccgctg gcgcagcagg
     2341 ggagggccgc gtgaacgccg cagctggttc aacgcatgtc ccccagcggc gcattcgcgt
     2401 gctgatcgtt gatgactcgg cgatcgtgcg gcaggtgctt caacgcctgc tgggcagcga
     2461 tccgcagatc gaagtcgtcg ccaccgcgca cgatgcgtat gtcgcgcgcg agaagatcgt
     2521 gcgctatgag ccggacgtcg tcacgctgga cgtgaacatg ccgcgcatgg acggcatcac
     2581 gttcctgcgc aagctgatgg cccaccaccc ggtgccggtg atcgtcgtca gcgggcagac
     2641 cagcgacggg acggccacag cgctagaagc gctgtgcgcc ggagcggtcg atctcgtctc
     2701 caaaccggcc ggtgaagccg acctggcggg gctgggatcg gtgctgatcg acaagatcaa
     2761 atccgcggcc cgcgcgcggg tgaagctggc gcccgcggcg gtcgtctcgt cggtccaggc
     2821 tgatgcctgc aagatgccgc gcattgcgcc ggcagcggca gccaatcaac ccgcgtcggg
     2881 accgagcgat ccactcatct tcgcgatcgg cgcatccacc ggtggcgtac aagcgctgac
     2941 cgaagtgctg acggcgttcc ccgaagacgc gccgccgacg ctgatcgtcc agcatatgcc
     3001 ggccaagttc actgggccgt tcgcgcagcg gttgaacaag ctatgccgcg tggagattcg
     3061 cgaggcagct aacggtgatc gcctgtcgcc gggaaccgtg ctgatcgcgc cgggcggatt
     3121 ccacatgacg ctgcgccgcc gggctggcgg gttcttcggc gtcgaggtgc gcgacggtcc
     3181 cgaattgttc caccagaagc cgagcgtcga ggtgctattc aactccgtcg ccaaagccgc
     3241 gggggcgaac gcggtcggcg cgattttgac cggcatgggc gccgacggcg cgcagggttt
     3301 gctccgcatg cgacaggccg gcgcgttcac gctcgcgcag gacgaagcca gctgcgtcgt
     3361 gttcggcatg ccgtgtgaag cgatcaagtg cggcgccgcc catcaggtgg tgccgctgac
     3421 cggtgtggcc gatgccatga tggcggccgc ccaacgacac gagcgcggcg gctctgccgc
     3481 gcgtgttcca atcaatcaac gaatgtcagc ctgagcaaag tcccattttt actcaacccc
     3541 gaaggaagga agacgtatgc agaatcaagc caccatgcaa cctccggcgc tcgcggcacc
     3601 ggcgaacgga aaaccggccg gcgtggccgc cgccgtcacg acaacgctcg acccgaagct
     3661 gatcgccccg ttcgtctcgt cgatccgcgc ggtcttcagc acgatggtcg gtgtcgagac
     3721 gaccgtgcaa cggccgtatc tgaagaacga tgaaacgcac agcttcgcgg tctccagcat
     3781 catcggtttc tccggccaag tggtcggttc ggtgacgctg agcttccagg aagaaaccgc
     3841 caagaagctg gtcgcgaaat tcgccggcgc cgaactgggc attcacgatc cggactttgc
     3901 cgacgccgtg ggtgaactgg ccaacatggt tgccggcgcc gccaagaagg acttgggggg
     3961 aactgcaagc atcaccgtgc cgagcgtggt gatcggatcc ggccacaaca tcgcgcgcct
     4021 gcgcgacgtg ccgtgcgtgg tggtgccgtg cacatcgccg ctgggcgatt tcacggttga
     4081 agtgagcatt aaacgcgtgt gagtgttact gcagatttga ttaacaccaa ttgagacgga
     4141 gctaactatg aagatcctgc ttgttgatga ctcgaagacg atgcggaaca tccagaagaa
     4201 gacgctggag cgcctgggga cggtggagtt cgtggaggcc ggcgacggcc tggaggcgct
     4261 gaccgccatc gccgcccatc ccggcatcga cctgatgctg gtggactgga acatgcccaa
     4321 catggacggg cacacgctgg tctgccgcgt gcgcgagaag gacaagacca cgcccatcat
     4381 catgtgcacg accgaagcgg aaaagacgcg cgtgatcgat gcgctgaagg cgggcgtgaa
     4441 taactacatc gtcaagccct tcacaccgga tgcgatgtcg gacaaggtgc agcagacgct
     4501 ggccaaggcc aaaggcgccg tggccgccgc cgcatgagcc atcgcggcgt gcgcgccggc
     4561 gctgtgatgc gcgcacgccg cgatatcggc gaaggagcct tccccatgca aactgatact
     4621 gagaaccagg tccatcacgc catcgagctg ctcgttgagc acgaagccgg tgaagagcac
     4681 tcgcattacg accggtccaa cttcctgttc gtcgaggcgg gccagccgat cgcgaagctc
     4741 gcagccgacg ccgagcacct gatcggcctg tcgctgaaga acgtgaaccg cacgccggag
     4801 ggcatgttcg tcgccactgt tgccggttgc ctgaaggcgg atcacgcgca cctttgggtg
     4861 gaacccggcc tgcgcgtcgc cggcaacgtc gatttctcca gcggcaacgt ggagtacaag
     4921 ggcgacgtgt gcgtgatcgg gaacgtcgtc gatctgttca aggtcaacgc caccggcgcg
     4981 gtgacgatcg cgggcacgat tgaagcggcc gaggtcagcg ccggaaccga cctgaccgcc
     5041 aagggcggca tcgccgggcg cgaaaaaggc atctgccggg ccaacggaaa gctcaccgcc
     5101 aagtacatct ccggcgccac cgtggaggtc ggcggcgacg ttcaggtgct ggacgaagtc
     5161 tcccacgcgc gtctcgtctg cggcggccga ttgaaggtca cgcatcgcac aatcgtcgcc
     5221 tcccacatca ccgccaacgg cggggtcgaa tgcgccaacc tcggcgcccc cggggccggc
     5281 gcgaagacga ttatcgaagt cggcgtcgac gcccaggtgc gcaagctcgc gcaagtgaca
     5341 ttccccgcgg tgcagcgcat gcgccacaag gcggcgcagg tgcggctgtc gattgagccg
     5401 ctgatgcgcg accagaagaa actcaccgtc gcccagcgcg agcgcgcgac cgaactgctc
     5461 tatcaagccg atgaaatcga gcaggaagcc aacgataagg acaaggccct gcgcgatgca
     5521 ttcgaacgcg cacaggccaa gcgcagcacg gagatcgtcg tcggcggcaa gctgttcgcc
     5581 gggacgatcg ttcgcctcgg ggagttggag cagacgatcg acaacaccgc cgacggtccg
     5641 ctgacgatca cgctgcgcga ccacgaaggc actcagcaga tcgccctcgt gcaaacccac
     5701 ggcgggtcaa cgagattcat cggtggccgg cccgtgcgcg atccggagat ggacgcgatg
     5761 cggcagcagt tcagcaaggc ggcatgagcg cgccagtcgc gcaagtgcga cccgggaagt
     5821 ccgcgtgctc atgcacagga gatgcatcac ttggatgtaa acctgatcaa cccgttcatc
     5881 atcgccgccc gcggcgtgtt tgacacgatg gtgcgcttgc cgctgatgag cggcaaaccg
     5941 cacgtgcgcg ccagctcgca ggcggtgaac cggacgttta aggtctccgt gcagattcgc
     6001 tttcacggcg cagccgaggg aatcgccgtg ctgcacatgt ccgaagcggt cgcgctcgcg
     6061 atggccacgg ccttcagcgg ggaaccttac ctctcgatca acgccgacgt gcacgatgcc
     6121 ctcggcgagg tggccaacct gatcatcggc agcgccaagg agcacctcgg ccccgacctg
     6181 cacatcagcg tgcccaaggt ttacgacgcc agcgaggtgc actgcccaac gggctctccc
     6241 gttgtggtga tccccttcga caccaccgcc ggccggtttg tgctggaagt gagcctgcaa
     6301 tccaatcccg ccgccaaagc cgtccgagcg gcgtgagatg atctcttact cacgcagctg
     6361 cattcgccgc gcgcctcgcc cagctccgcc tgaacgccgc gactcatcgc gcctttaccc
     6421 atcctgatta aactcgaaat taccaactcg tgcgctagcc gaaagtccgc ggcggctacg
     6481 gagcccgcgg ttgttcaagc gcggctgcac cgatgcgctt cctcaaggct gacctcacat
     6541 agcatcttcg aatcgatacc tcgcccgagc ctgccccaga agaccttcaa tcattcacgc
     6601 caatgcggct gctcatgccg accgttcatc ccgcgatggc gccgatcgta gccaatcacg
     6661 cccccgtgaa cgagcggaac ttcgcgatca ccaggtggtc cgtctggtgg ctcgttgcgg
     6721 ccatccccag gtacaccgtc tggcccagcg ggaccgtgac ggcggggccg atcgggttcc
     6781 acgttgtgcc atcgatgctg gcgtacgttg tgtacgtgtc gccgacgcgg accatgcgca
     6841 cccacttctg cggggcgggg ttgttggaga tcgctgcact ctgcgtcgtg gtggcgccgg
     6901 cggtggggcg atcggtcgtg atgaaaccga acggaccggc cagcgtgaag gtgtagaggt
     6961 tggccgaacc agccgacaga tcggtgcgga ccatgatgcc ggcgcgggcg tagccgctgg
     7021 tggtgccgtc accgttggac agagaggcga tctgcacctt cacgtcgaag tcgccgctca
     7081 cttgcttgta gacgtagcgg aactggtcgg cggtgccgtt gatgttggcg ccgctggcgg
     7141 ccatgtcgta atcgctgccg ggcgtgatgg tggtggtgga cccggcgatc ggcgggttgc
     7201 cgatatcggc gctggtgaag gtgctctgca ccgggacgct tgcgctggcg gtcgccggag
     7261 gcgtcgattc gttcgccgag gtatcgacgg cggtcaactg atagaacgac atctgccccg
     7321 cgggggcggt ggtgtcgttg taggtcacgc cagtcaggaa cccggtggtg ttcagcttca
     7381 cgaacgtgcc gccggcggtg ggggcgcgat acaggttgta cccggccaga tcgggctcgg
     7441 tgttggcgga ccacgtgagc gaaacgccac ccgcgctacc aacggcgccg aagttcgtcg
     7501 gcgtggccgg cggggtggta tccgtcgggt tcggccgcag cgcgttcgcg ttggccggcg
     7561 tggtcgattc gttgttcgag gtatcgaccg ccgtcagatg atagaacgtc accgcgcccg
     7621 agggggcagc ggtgtcgttg tagctcgtgc cggtgatcga gccgcccctg ttcacgagcg
     7681 tgaatgtacc ggtggcggac gtcgagctgt acaggttgta gccggcaagg tcggactcgg
     7741 tattcgcgtt ccaagccagc gcaatgccgg ccgtgctgcc ggtggcggtg aagttggccg
     7801 gcgtcgccgg cgcggtggcg tcggggcgca gcgcgctggc cgttgcgaac gtggattcgt
     7861 tattcgacag atcgacagcg ctcaggcgat agaacgtcgt cgcgccctgc actgcggcgg
     7921 tgtcgttgta ggtcagcacc ttcagcagcc cggcggtgtt caacttggtg aacgggccgc
     7981 ttgcgctggg accactgtag acgttgtagc cggccaagtc tggctcggtg ttggcggacc
     8041 aggtgagggt gattccgccg gccgcgccgc tggcggcaag gttgctcggc tgcgcgggcg
     8101 gggtgatgtc gttcgggctg ggccggaacg cattggcact ggtcggcgtc gagctgttgc
     8161 ccgacgtatc gaccgctttg atttggtagt agttggtcgc gccgacggtg gcgctggtgt
     8221 cgttgaagct attagcagtc accaagccgc tggtgttgat cttggtggtg aacggcccgg
     8281 ccgccgacgc ggcgcgatag acgttgtaac ccgccagatc cgtttcggta ttggccgtcc
     8341 aagacagcgc gatgccgccg atgctgccgg tggcggtgaa gttcttggga gtgctgggcg
     8401 cggtggtgtc cggccgtagc gcctgggtgg tggcgaagct cgattcattg ttcgacttat
     8461 ccaccgccgt caggtggtag tacgtcgtca cgctcgcggt ggccagcgtg tcggcatagc
     8521 tcgtggtcgt gatcaagccg ctcttgttga cgatggtgaa cgtgccagtc ggcgacaacg
     8581 agctgtagac gttgtagccg gccaggtcgg attcggtatt cgcagtccaa gacaggttga
     8641 caccggacgt cgtgccgttg gcgatgaagc cggtcggctg cgcgggtggc gtgatgtcgc
     8701 cgccacctgt gcgcgtcgcg ctagtggacg acgccgacga ttcgttgccg gagaagtcaa
     8761 cggccgtgac gtgatagaag gacgccacgc ccgatagggc ggccgaatcg acgtaacccg
     8821 ccgaagagag caactgcgtc gtgttcagca gcgtgtaggt gccggttgcg ctggtcgcgc
     8881 gatagacgcg atagccggcg aggtcggtct cggtgttcgc cgcccaggtc aatgtgatgg
     8941 cggtgctgct gccgctggag ttcagattcg ccggggcggc cggcagcgtg ctgtccttgt
     9001 gcaccgcgtt cgccgccgtg ccggccgatt cgttgccgga tgtgtcgaca gcccgcagct
     9061 ggtagtacga cggcaagcta agctgcgcag cagtatcgac gaacgacagc gcggtcaacg
     9121 gtgtcgagtt caacttcgtc aaggcgccac cgctggtgtt gctgcgatac aggttgtaac
     9181 cggccaggtc cgaatcggtg ttggccgacc aggacagcgt gatgttcgcg gtcgatccgt
     9241 tggccgagaa ccccgtcggc accgccggtg gagtcgtgtc caccaggcgc gtcgcgttcg
     9301 ccgtcgcggc agtcgattcg ttgctcgatg catcgaccgc cgtcacctga tagaacgacg
     9361 cctgccccgc gggggcggtg gtgtctacaa agttggtgcc ggtgatcaac ccgctggtgt
     9421 tcagcttgaa gaacgtgccg gtggcggagg tggaacgata cacgttgtaa ccggccagat
     9481 ccgcctcgat gttggcggtc cagctcagcg tgacgccgct gctggatccg gttgcggtca
     9541 atcccgccgg cgcggcaggg gcgaccgaat cggccggccg agtcgcgctg gcgacggcgc
     9601 tctgcgccga ttcgcaaccc caggtgtcgg tcgcggtgat cttgtaatag tacgtgctgc
     9661 ccgcggcggc ggtggcgtcg ttgtcgctcg ccgtcgccac caccgaactg ttgatcttca
     9721 cgaacgtgcc ggtgctggac gtgctgcgat agacgttgta cccagccagc agcggcgcgc
     9781 tgacggcggt ccacgtgagc gagattcccg aaccgctgcc cgttgccaca ggcgtcgtcg
     9841 gcgccgccgg tggggcggga cgcatgttcg aaatcaggaa gacgttgtca ttgtaatcgt
     9901 aattgatccc ggcgtagtcc atcgacatca gccaggtgtt ggggatcaga tttccgctac
     9961 gatcgcgcgc cggccagaag cgcaagtgat ggccctggtc ggtcgtcgtc ggcttgttgt
    10021 tcagcgagtc atcgctggat tcctgcttgt cgatgttgaa gccaaaggtg ttgttcgtgc
    10081 tgaacgtgcc gaacgccgcc gcgtgcgtcg tgtcgttcga ctgcggtagg aacgactgcc
    10141 cgtcggcccc cacggcgttg gccacggcgt tgtatgtcgt cggcgagcct ttggagaacc
    10201 aatagagcgt gtcctggttt ccctgcgtgt ggaacgacgc cagctgccgc gcgctcaccg
    10261 gcaagttcgt gtcggcgcgc acccagtaat tcgacagcac ctcttcaccg accgtggcga
    10321 tcttcccgcc ctggttcatg ttctggccgg agttcaggat cgtcgtcttg tagccaagca
    10381 ggttgatgat cgtggagacg ctcggctcgg tgtacgcgcc ggtcgtgggg ttgctgttgc
    10441 cccacaactg ccagaaccca tccagctgca ccgttcgcgt cgcgccgttg atcgcgttgg
    10501 tgctgatcgt cagcgtgccg gtgtcgctcg cgttgttgcc cgtgtgcgtg ggcagatacg
    10561 tgacgacgac atcggcgaac gaattcgctg cgatcgacga ggggaatgtg cccaccgtga
    10621 acccggcgtt gctcgacgtg atcgagttca ccatcatcgc ctgatcgccc gtgttccaca
    10681 cgcgcaccgt cgccttgttg tggaagacgt tcggatgcgt cgcgttggag ttctggatgt
    10741 cgttcagcgc aagacgatcc acgaacggcg cgccgtcctg gttctgcagg cccagctccg
    10801 gcccaaccgg cgccgcctgc acgttgcgga tgatgccgac aacgtcgttc gagtcgtagc
    10861 tcggcgcgat gccgtaatct tccgtcgcga agatgaacgc gttgggcacg atcgagccgt
    10921 cgctgttctt cagcgggtag aaccgcacct tgcggttgtt gtttgacgtg tcgtaggtgt
    10981 tgaactcgtc ttcgctgtac gcgatgcgcg gggtgccgac cgacgtcggg aaggcgtcga
    11041 acgacgaata caaaccgaag acggacgaac cgggatcgaa gctggtcgcc ccgatcggtg
    11101 tcacgtgcac cgactgcgca tcggccgtgc cgacgttgaa cacttccgtc ttgctgtccg
    11161 gtgtgccggc ggtgtagtac ccgaaaccgg ccgacggcgt gatgctcgcg ctgaacaccg
    11221 ccagcggcgt caccgtcacc ggtccggtgc ccgccttcac cagccgcggc atgacaactt
    11281 cgtcattcgg cgttcccggc ggcgtggtga acgccgtcac gctcgggtcg gccgtgccgg
    11341 tctggatcgg gatctgatac agatccagga tccgctgcag cgatggttcc ttatttccgc
    11401 ccaatcctgc cgtcgcaaga gcgcgcaggt tcacatccag cagcggcgtg ttcgggtcgt
    11461 tggtcttgat ctccagcgtc gccgtgtgca ggcccagggc cgacgtgctg gccgggttga
    11521 acgccaacgt gatggtgatc gaaccaaacg cggggatcgt cgccggcagt gacggcttgg
    11581 aaacgatcgg gaacaagctg ccatccgcgc cggtgacggt cagcccgtcc gacggcagcg
    11641 ccagcggcgc gctgccggtg ttggtgatcg tcagcgactg cgccggtccg gcgctgccgc
    11701 ccaccggatc gttgaagtac atcgtcgagg cgctgaccga cacctgcgcg ccgctgttga
    11761 tcgggcgcag cagcgtcagc ttcatcccac ccagttccgc gacgtacagg aaccccgtgt
    11821 gcggatcttc gaccacgtca atcgggttgt tgaaccccgt gaacccggcg atgccggttt
    11881 ccgtctgcgt cacctgcccg ttcgcgccgg tgcggatcag cagcagatct ttcccgccgc
    11941 tgtagcgcgt tgccaggatg gcgtgatcca gcgcgccgtt gaacgccgag ccgtgatatt
    12001 cgagcgcgcc gtcgggcgaa tagtgcacgc ccaggtccag cgcgacgccg cggtaattcg
    12061 ggtcggggtt ggtgccgacc gggtacgcat tttggattgg gaaattcggc cccagcggcg
    12121 tcgtcggatt gccgccgttc atgatgaact cgttgcgcga cggattgggg tgaccgtagt
    12181 acccgccctt ttgaatgtcg aagagccagt cgtcctcggc gacggccacg ttgttcagcg
    12241 ccggtcccga cggcgtcacg cccgccggtg tcgccgggac gttcccgccc gcagccgagc
    12301 cgttggtcgg cgcgtatagg tgcccgttgc tgtcgaagac caggtcataa tcgttgcgca
    12361 caccggtggc gtagagcgtc agcgccgcgc ccgccgcgaa tgggttatac gccgtctgcc
    12421 ccgcggggag gctgtcggtc tgcacgttca gcgctttgcc gttggtcgcg acgtagctgc
    12481 gaatcgcggt gacatccagg cgcaggatcg ccgcgttaag caggtgttcc tgccgcaggc
    12541 cccacaccgg gtcaggcgcg cccatcgcgc tgttgctgcc ctggccccaa tagagcgcgc
    12601 cgtccggtcc gaagaccggc tggttgttca agtggtcctg gctcgaccgt ggcaggccga
    12661 tcaccatgtc cttgtagttc ccccacgacg tgccgctgcc gctgatcgta ctcatcttgc
    12721 cggtgaaatc gggcgccccg tagatgatgc cattgccgct gctgacccag atcgtcatat
    12781 tcgacgccgt cgaagtcgga tcgaacgaga tgccggtgat caatcgtccg ccggcgttgc
    12841 tcgggtcgtt gatgaccgtt tggatgtcga acggattgcc cagcgtgccg tcggtgttca
    12901 cagggaagcg gaagatgtgc ccgttcaacg tgctggcgta cagcgcgtca tccggtccca
    12961 tcgtcacgcc ggtgaacatc tggttgactg ccgtcggcag cgtgactttt tcgaatgcga
    13021 tgttcggatc ggtcgtgatc ggcgctgaac cggtcgtgaa cgtgatcgag aacggctgga
    13081 acgtcgcgcc cagcgcatcc ttcaatcccg cgttgaccac gaacgtgtag gtcgtgctgg
    13141 catcgagcgg ggtaaacggc tgcagcacga cgacgctgcc cgcgccgtcg gtattcacag
    13201 ctgcgtcaac cagctggtgg tcgaacgtgc ggaacagctg caccgtgttg gcgttcagcg
    13261 tgctcacgtc gatgccggat gccaggttcg gcaagttcag atcgcaggag acgaacgcgt
    13321 tacgcgcaac ggcggtcgcg ccgttgatcg gcgtgacgct gcggatcgac gggttcggcg
    13381 tcgggccggt cacctggttg tcgaactcga tctccgccac gtcgccgtgc gaaccggtgg
    13441 ggccgatgta tcgcaggtaa cggaacggcg tgctgacgcc ggtggtcacg gtcgtccacg
    13501 cgccgacggt gggggcgctg ggaaccgtgt agagattcac gacgccgctg gagaagtcgg
    13561 cggtgtcgga tccctggaaa atcccaccga ccatgcgcgc agcctgtccg ctgcgcggcg
    13621 cgaatttgat ctgcgctgcg acgttctccc cgccggtgtc gatgccggtc cacgcgaagt
    13681 cggcgacgct ggcgtcgaac gcggtgttga acgagaaatc gaacgccttg gccggtccgg
    13741 tgctgctgcc gctgccggcg ctgccgatgg cggtgccgat caatttcggt cccgtctgcg
    13801 ccggtgtcgt gccggccacg ccgtcgaatt cgatctcggc cacgttgccg tgcgaactgg
    13861 tggggccgag gtatcgcacg tatcgatacg tgccggggtc ggtgacgggc accgtcgtga
    13921 aggaacccac cgcgggcgag gtcgtcaccg tgtacaggtt caccacgccg ctggagaagt
    13981 cggccgtgtt cgaaccttgg aagatgccgc cgaccattcg cccggcaaag ctgctgcgcg
    14041 gcgcgtactt gatctgcgtg atctgcgccg gcgcgcccag gtccataccc gcccaggcgc
    14101 cgtcgggttg ctgcgcgtca aagtagtccg acaggctccc gtcgaacgcc ttggcgatcg
    14161 tgttgcccga attgttgtac gagcccggcg tgccgatgac ggcgccggtc agcgcattgc
    14221 ccgacggcgg tggcgcgctc atcacgccat cgaattccag ttccgccaca ttcccgtgcg
    14281 agctggtcgg gcccagatag cgcacgtagc gatagctgcc cgtgtcgctg atcgactgtg
    14341 tggtaaacga tccctgaacg ggtgccgcgg tgatcgtgaa taggttgacg acac
//
LOCUS       DATIMH010000465        11265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4437768, whole genome shotgun sequence.
ACCESSION   DATIMH010000465 DATIMH010000000
VERSION     DATIMH010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4437768"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3594)
                     /locus_tag="VLI90_08025"
     CDS             complement(<1..3594)
                     /locus_tag="VLI90_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14192.1"
                     /translation="MNLRPKTKRRLLVLIAAIGLLAGGITAFVNVQLHRYEAARLQFR
                     KQGMAAYNRGDYTGALDQFSRYLRNDTNDPEALYAYAVARSRVPRPDLAHLTEAKSLF
                     NRYLEQVPGDANAQHQLLEIYQKLNYHAEAVSLADTLLARDANDLPAMKAKLHALLGQ
                     SKYDEALTLCKRVNELAPLDLDAQKLTLALLRKLQRPPAELTARADVMLAGHPNDPRF
                     ELLRAVAASFVGDDEGAKKWVMTAATRPPPDPAFVRELTGVFDRIGMYDESRDLLEKS
                     AQQSAASDPQLLASLAQRLWESGQYADVLDRLKNVNASEPQASSALLAYKALALYCLA
                     EMPDHPSTRPAATTGPAARAAADAIVEALTKRPGDGIAAGWTATLKTRFANPPLPSRD
                     AVKAYQDALPLDPDNAVTRYYLGAAYLALGENEFALRYWQMASQISPAWIEPHVMMAR
                     TLLSSGRLAEAQGEAEIAARRSPNLLAVQATRAVIAYTQLQPSADHEKTERLLEFVRR
                     IQVELPGEPQTLPIYIALLTRAGHRDDAIASIKSALAAKPPLQSDTLLKLAQIDVAER
                     LELAQPIIDALSSDHIDTAARAFDRAMALASYGRSVDGLKFITTAFDSAVKAGVATQG
                     DAPQWQLAVAKYREAIGDAGVADAWAALGDANPKDIAIQREVLAARSAWSNRELIDRT
                     IDRVKALTGDEGIEWRLARARWRLGAPDDPRNNANAAASMMADVVRAAPNLFDPLVVW
                     AQALEKLGDTTGAIDRLRTAAVLSHGDVNVLMYLATLLQDRGNVVETRQILEQLSQKP
                     TLSAAGRVQVARMYSMQGQYNEAVAVLRGGNVADGGDVERDLLLAQLLQAQGKGDEAA
                     HVYDAVLQSPALSDAGVRAVAWFYARRQTMAKAEQALAKLNAMQLSPGVKELTLAAFT
                     EQFNPKLDAALSHYQAATLAAPSNPATWKGLAGFHLRHRSYDAAAAVAEQGLKINAGD
                     AQLAALAAGARSLIDIKGGSELQPAINAFAANPIDPPAQQTIEALAATQSKRNTPDRA
                     LLRLRDVADLYPKFLPAQMLLAERYFTAGRFEDADAIATRAMQAMPTEADPAHLLSVI
                     AATAGHWDRALSAATEWRNRSLDQPLYADQQIAEAKLHLNDPVGALQQIKPYLQQAAD
                     HPDDFPQIVRQHAQALALQGQVADAQATLDPLLSKSPTWRAIWLDVAAATAR"
     gene            complement(3685..5178)
                     /locus_tag="VLI90_08030"
     CDS             complement(3685..5178)
                     /locus_tag="VLI90_08030"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HSV14193.1"
                     /translation="MSRAEVAIPSLTPPAPNLTLVSPPGPGAPPAPSQLRTIWGLDPI
                     QLHTRYWASNGVQVVRQGEPSEIVKHAELFLLTEPSTLTMFRLADVMDALNWIKPQVL
                     FVRLHDSRERGYREFVVTDEFDQFVRFERRYDASGRLTRVVLTPDRDVAKLWQSAPDG
                     ITGWRRLRRFIPRTDRATVSINGNLYDRSSNREVAFFIHDLVARWRRPDSTVLRAMPA
                     QGQAWRDAQAKIDPNAKFIGPVWVGCGRQVAAETTVVGPAVIWDDPENRPVNDDIHWL
                     HIEPSPAPDDAPQPRPGSPLDRAFKRAFDIAFATAAIIFTLPLYPLIMIAIWIEDGRP
                     FFFSHKRETLGGREFPCVKFRSMRKDAEKMKAVLKKLNRADGPQFFIENDPRLLRVGR
                     FLRKYNLDELPQFWNVLVGDMSIVGPRPSPHKENQYCPPWREARLSVRPGITGLWQVK
                     RTRRAGSDFQEWIKYDLEYVEKRNWWLDLKIIWQTIMNILGKVSRSD"
     gene            complement(5317..7551)
                     /locus_tag="VLI90_08035"
     CDS             complement(5317..7551)
                     /locus_tag="VLI90_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSV14194.1"
                     /translation="MSKEIQLSNNYPAAADYWTQGQAAVGGPVNSGGGGSSAPRTSPL
                     RRAHRLLRGRYPLAFLMAGIGAIAGCIALYTLLPPKWQSDGMISVNPMIMTPGLHDES
                     VPLFTMVMRSQAALIESTSVAEEALKDPELMNALTKVYGSPSAMSATDFGTALTTDRQ
                     KDGNIIKVSFADKNKEVAQAGAHAAIRAYMKLYGDGDSNARTIKIKFNTDEQRRLNSD
                     LSFARSQLVAWSQSYGTTDLGPLFDAMQGEELKLEAELDQATLQYKAATDLQSKKPTT
                     NPNDREFIYKQIAVVDLQMRQLLAERGHTVDSLEQLVFKLGRKHPLYAAEYLKYQQAN
                     RAVEQYARDYLNAHAGQIPFGLDDPANGGGNLAMMKTKIDQLQSRLDDKKKQVLLIGE
                     LRNRIAEKQRDISKNEEGLKVVEGRIQSLEAEQKLAQSVHVNDYGSEAINTGDKKRVI
                     AAGAGFMFGGLIPVGLLLLYGMTDARFRYSDETGESDMAGVTLLGILPNLPDRLSDPQ
                     QAGIAAHCVHQIRTMLQISRTNDEPQVIAVTSAAPGDGKTSLSLALGLSYAACGARTL
                     LIDCDLVAAGLTHRLNVNSPEGVLEAVANRALLEFVRTTDIADVAILPVGTTHAHHAS
                     TLSPVALRRLLSESRKHFDVILVDTGPILGSIEASLVCAAADRTILTVARGQQRPLVE
                     RSIGHLQAIGARLAGVVFNRAQARDFERSLSGLALRSRPANGHAPANGPTATAVAGSF
                     KRSA"
     gene            complement(7548..8240)
                     /locus_tag="VLI90_08040"
     CDS             complement(7548..8240)
                     /locus_tag="VLI90_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14195.1"
                     /translation="MRNARVIFAAPAACLALLLGMVAESSTRITPADAGPYHERAREA
                     LESIPMQIGSWSGQEEKIAPEAIRLLHPNFIREWKYSDNAPNPRWYDRWASLLVDQCR
                     DAGDMNGHWPPNCYVNNGEELMFSQERDWRVGDLLITGKEYHFVKTSPTESTRTAVYN
                     FLIVPGRGILRDMKDVRKAAEDYQRRYYGAAQFQLLMNADLPQQERDEIFTTLMQPCV
                     PVIKTLMSGGIR"
     gene            complement(8319..9194)
                     /locus_tag="VLI90_08045"
     CDS             complement(8319..9194)
                     /locus_tag="VLI90_08045"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HSV14196.1"
                     /translation="MTSRLFRSDRWTLAHVFAATVMAAIGVAVTWPAWANIYHIASRD
                     EESSHVFLVPVVALWMLWARRVRFRYCKPTGTILGPILVGVGWAISSFGFYHGIQSFW
                     HGGAVIVVLGCIVAVLGKHAILRFLPVVAVLAFLVPVPGEIRLRVALPLQNWTARIEQ
                     TMFEVLGTEVVRSGNQLTINGVPVHIIEACNGLRMVFALILVSYAFSYGLPLRNSVRI
                     VILLVSPIAAIFCNLIRILPTVWLYGNASRAVATAFHDYSGWLMLPIALLMLLGIIRL
                     LRWAMIPVMRYTLAS"
     gene            complement(9191..>11265)
                     /locus_tag="VLI90_08050"
     CDS             complement(9191..>11265)
                     /locus_tag="VLI90_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HSV14197.1"
                     /translation="GKLRSMYFRDGDNSWDAADASDLATAKAAPALVARPSRRDGYKR
                     NGGTVGQNGKLLPFIHLHGVKLHRVTERECIEHILDELDARRGGVVVTPNLDHLRRCV
                     RDLNFGALVAEADLVVADGMPLVWASRLQGTPLPERVAGSNLISSLSAAAAGRAKSIF
                     LLGGAEGTAEGAGAILRQRHPELKIAGAFCPRLGFEDSPKQMAEIAQLITDAKPDIIF
                     VALGSPKQEHVIARLRSTMETAWWLGVGVSFSFLTGHVKRAPRWMQVCGIEWLHRLCQ
                     EPRRLFHRYIVVGIPFASSLMSRAIVRGIPKRLGWTQPAPASFPTSFARVESPTSPTA
                     PAVAANTATTVAGDSVNDRLTDRARRMATTLDQSVSTLSRLKAIILLGGHVRPTPLST
                     SIGRSLLDLPLDEEGSVLNHWLAHASELAKVAGLEKLPVRVMVDRNSPEPISASVKYF
                     GTFRVERDQSEYRGTGGVLRDLAENYADDDLILVANAAQILLDPLVSLAASLDQRRGD
                     VAIISHRDGTPSGMMLVRCKTLRLIPKTGFIDMKEQAMPTIAQQYAIEVVHRRRPTGL
                     PIRSVTDYITAMRFYHMRRAGKTTLTDPLAEDWQPAFGIVEEGARVDSTARVHDSVVL
                     RGGIVEAGAVLVRSVVCPSGLVRPDQTVVDDFVRPVNSPRASGPHLSGTATTPAAALD
                     PADKGKAA"
BASE COUNT         1891 a         3577 c         3736 g         2061 t
ORIGIN      
        1 gcgcgcagtg gcggctgcca catccaacca gatcgcgcgc cacgtcggcg acttgcttag
       61 cagcggatcg agcgtcgcct gcgcatcggc cacctggccc tgcagcgcca gcgcctgcgc
      121 gtgctgccgg acgatctgcg gaaaatcatc cggatgatcg gccgcctgct gcaggtacgg
      181 cttgatctgc tgcagcgcgc cgactggatc gttcaaatgc agcttcgcct cggcgatctg
      241 ctggtcggcg tacagcggtt gatccagcga gcggttccgc cactccgtgg ccgcgctgag
      301 cgcgcgatcc caatgaccgg cagtggcggc gatgacgctc agcaagtgcg ccggatccgc
      361 ttccgtcggc atcgcctgca tcgcacgcgt cgcgatcgca tcggcgtctt cgaatcggcc
      421 cgcggtgaaa taccgttcgg ccagcagcat ctgagcgggc aggaatttcg ggtacagatc
      481 ggccacgtcg cgcaggcgaa gcagcgcgcg atcgggcgtg ttgcgcttgg actgggtcgc
      541 cgccaacgct tcgatcgtct gctgcgccgg tgggtcgatc ggattggccg cgaaggcatt
      601 gatcgccggt tgaagttcgc tgccgccttt gatgtcgatc agcgaacgcg cgcccgcggc
      661 caaggccgcc agctgcgcat cgccggcgtt gatcttcaat ccctgctccg ccacggcggc
      721 cgcggcatcg taggaccggt ggcgaagatg aaagccggcg aggcctttcc acgtcgcggg
      781 attcgatgga gcggccaacg ttgccgcctg gtagtgtgac agcgctgcat ccagcttcgg
      841 gttgaactgc tcggtgaacg ccgccagcgt cagctccttc acgccgggcg ataactgcat
      901 cgcgttcagc ttggccagcg cctgctcggc cttggccatg gtttgccgcc gcgcgtagaa
      961 ccacgcgacc gcgcgaacgc ccgcgtcgct gagcgcgggc gattgcagaa ccgcgtcgta
     1021 cacatgcgcg gcttcgtcgc ccttgccctg cgcctgcagc aactgggcca gcagcagatc
     1081 gcgttcaaca tcgccgccgt cggcgacgtt tccgccgcgg agtaccgcga cggcttcgtt
     1141 atactggccc tgcatcgaat acatccgcgc gacctgcacg cgcccggcgg cgctcagcgt
     1201 cggtttctgc gatagctgct ccaggatctg ccgtgtttcg acaacattcc cgcggtcctg
     1261 cagcaacgtc gccaggtaca tcaggacgtt gacatcgcca tgcgaaagga cggcggcggt
     1321 gcgcagacga tcgatcgcgc cggtggtgtc gccaagtttt tccagtgcct gcgcccacac
     1381 gaccagcgga tcgaacaaat tcggcgcggc gcgaacgacg tccgccatca tcgaagcggc
     1441 ggcgttggcg ttgttgcggg gatcgtctgg cgcgcccagt cgccagcggg cgcgggccag
     1501 ccgccattcg attccctcgt cgccggtcag cgctttcacg cgatcgatcg tgcgatcgat
     1561 cagctcgcgg ttgctccacg cagatcgcgc ggccagcact tcgcgctgaa tcgcgatgtc
     1621 cttcgggttc gcgtcaccga gggccgccca cgcgtcggca acgccggcgt cgccgatcgc
     1681 ctcgcgatat ttcgccacgg ccaactgcca ttgcggcgcg tcgccttgcg tggcgacgcc
     1741 ggccttcacc gccgagtcga acgcggtggt gatgaacttc aatccgtcaa ccgaccggcc
     1801 atagctcgcc agcgccatcg cgcggtcaaa agctcgtgcc gcggtatcga tgtgatcgct
     1861 cgacaacgcg tcgatgatcg gctgcgccag ctcgagccgc tccgccacgt cgatttgtgc
     1921 gagcttcagt aacgtatccg actgcagcgg cggcttggcc gcgagggcgg atttgatcga
     1981 cgcgatggca tcgtcgcgat ggccagcgcg ggtgagcagg gcgatgtaga tcggcagcgt
     2041 ctgcggttcg ccgggcaact ccacttggat ccggcgaacg aactccaaca gccgctcggt
     2101 cttctcgtga tccgcgctgg gctggagctg cgtatacgcg atcaccgcgc gcgtcgcctg
     2161 caccgcgagc agattgggcg atcgacgggc ggcaatctcc gcttcgcctt gcgcctcggc
     2221 caggcggccg ctcgacagca gcgtgcgggc catcatcacg tgcggctcaa tccacgccgg
     2281 ggaaatctga ctcgccatct gccaataacg gagggcgaat tcattttcgc ccagcgccag
     2341 atacgcagcg ccgaggtaat agcgcgtgac agcgttgtca ggatccagcg gcagcgcgtc
     2401 ctgatacgcc ttcacggcgt cacgcgacgg cagcggtggg ttggcgaagc gcgtcttgag
     2461 cgtcgcggtc caccccgcgg cgatgccgtc accggggcgc ttcgtgagcg cttcgacaat
     2521 cgcatccgcc gccgcccggg cggcggggcc ggtggtcgcg gcggggcgtg tgctggggtg
     2581 atcgggcatt tccgccaggc aatagagcgc cagcgctttg tacgccagca gcgcagaact
     2641 cgcctgcggc tcgctggcgt tcacgttctt caatcggtca agcacatcgg cgtattgccc
     2701 ggattcccac aggcgctggg cgaggctcgc gagtagttgc ggatcgctgg cggcggactg
     2761 ctgcgcggat ttttcgagca gatcgcgcga ctcgtcgtac atgccgatgc ggtcgaacac
     2821 gccggtgagt tcgcggacga acgccgggtc cggcgggggc cgcgtggcgg cggtcatgac
     2881 ccactttttc gcgccctcgt catctcccac aaaactggcg gcgacggcgc gcagcaattc
     2941 gaagcgtgga tcgttgggat gcccggcgag catcacgtcg gcgcgggcgg tcaactccgc
     3001 gggcggacgc tggagcttgc gcagcagcgc gagcgtcagc ttctgcgcat ccaggtcgag
     3061 cggcgccagc tcgttcaccc gcttgcaaag cgtcagcgcc tcgtcgtatt tgctttggcc
     3121 cagcagcgcg tgcagcttgg ccttcatcgc cggcaggtcg ttggcgtcgc gcgccagcag
     3181 cgtgtcggcc agcgacaccg cctcggcgtg ataattcagc ttctggtaga tctccagcaa
     3241 ttgatgctgc gcgttggcgt cgccgggcac ctgctcgaga tagcggttga agagcgactt
     3301 cgcctcggtc agatgggcca gatccgggcg gggcacgcgc gagcgggcga cggcgtaggc
     3361 gtagagcgct tcggggtcgt tggtgtcgtt gcgaagatag cggctgaact gatcgagcgc
     3421 ccccgtgtaa tcgccgcggt tgtacgcggc catcccctgc ttgcgaaact gaagccgcgc
     3481 cgcctcgtag cgatgcagtt gcacgttgac gaacgcggtg atgccgccgg cgagcaagcc
     3541 aatcgcggcg atgagaacga gcaagcggcg tttggtcttg ggacgaaggt tcatggtgtc
     3601 aaacaacgaa ggatgagcga tgagcgacga actgccactg aaaagctttt gctcatcgct
     3661 ccacgttcat cgttcatcgc tgtattagtc cgaccggctg actttgccca ggatattcat
     3721 gatcgtctgc cagatgatct tcaggtccag ccaccagttt cgcttctcga cgtactccag
     3781 gtcgtacttg atccactcct gaaaatcgct gccggcgcga cgggtgcgct tgacctgcca
     3841 caggcccgtg atgccggggc gaacgctgag gcgcgcttcg cgccacggcg ggcaatattg
     3901 gttttccttg tgcgggcttg gccgcgggcc gacgatcgac atgtcaccga ccagcacgtt
     3961 ccagaactgc ggcaactcgt ccaggttgta cttgcgcagg aaccgcccga cgcgcagcag
     4021 gcgcgggtcg ttctcgatga agaactgcgg gccgtcagcg cggttcagtt tcttcagcac
     4081 ggccttcatc ttctccgcgt ccttgcgcat cgagcggaac ttcacgcagg ggaactcgcg
     4141 cccgccgagc gtttcgcgct tgtgcgagaa gaagaacggc ctgccgtctt cgatccagat
     4201 cgcgatcatg atcagcggat acagcggcag cgtgaagatg atggcggcgg tggcgaaggc
     4261 gatgtcgaac gcccgcttga acgcgcgatc cagcggtgag cccggccggg gctgcggggc
     4321 atcgtcgggc gcgggcgatg gctcaatgtg cagccagtgg atgtcgtcgt tcaccgggcg
     4381 attttctgga tcgtcccaga tgacggcggg gccgacgacg gtggtctccg cggccacttg
     4441 tcgcccgcag ccgacccaga cggggccgat gaatttcgcg ttggggtcga ttttcgcctg
     4501 ggcgtcgcgc caggcttgtc cctgtgcggg catcgcgcgc agcacggtgg aatcaggccg
     4561 gcgccagcgg gcgaccaggt cgtggatgaa gaacgcgacc tcgcggttgc tcgagcggtc
     4621 gtacaggttg ccgttgatgc tgacggtggc gcgatcggtg cgcgggatga accggcgaag
     4681 gcgtcgccag ccggtgattc cgtccggcgc gctttgccaa agcttggcga cgtcacggtc
     4741 cggcgtcagt acaacgcggg tcaatcttcc cgaggcatcg taccggcgtt cgaagcgcac
     4801 gaactgatcg aattcatcgg tgacgacgaa ctcgcgatac ccgcgttcgc ggctgtcgtg
     4861 caggcggacg aacagcacct gcggtttgat ccagttcagc gcgtccatca cgtcggccaa
     4921 gcggaacatc gtcagcgtgg acggctcggt cagtagaaaa agttcggcgt gcttgacgat
     4981 ttcgctgggc tcgccctggc gaacgacctg cacgccgttg ctggcccagt agcgcgtgtg
     5041 cagctggatg gggtcgagcc cccagatggt tcgaagctgc gacggggccg gcggcgcgcc
     5101 ggggccgggg ggcgaaacca aggtaaggtt cggcgccggc ggggtgagcg acgggatggc
     5161 aacttcggcc ctgctcatca gacttcattg tctccattcc agagcgagtg gaaagagcaa
     5221 tacggaagtc agaatgcaga atgaagaatg aagacgggaa acacacgtcc ttccattctg
     5281 cattctgcat tcatcattct gcgttggatc acgcaatcac gccgaccgct tgaacgaccc
     5341 cgcgactgcc gtggcggtcg gcccgtttgc gggggcgtga ccgttggcgg ggcggctgcg
     5401 cagagccagg ccgctcaggc tgcgctcgaa gtcgcgcgcc tgcgcgcggt tgaagacgac
     5461 gccggccaga cgggcgccga tcgcctgcag gtggccgatc gaccgttcga ccagcggccg
     5521 ctgttgcccg cgggctacgg tcaggatcgt gcggtcggcc gcggcgcaca cgaggctcgc
     5581 ctcgatgctg ccgaggatcg gaccggtgtc gaccaggatc acgtcaaaat gcttgcgcga
     5641 ctcgctgagc aaccgccgca gggcgacggg cgacagcgtg ctggcgtgat gcgcatgggt
     5701 cgtgccgacc ggcaggatcg ccacgtcggc gatgtccgtc gtccgtacga actccagcag
     5761 cgcgcggttc gccaccgctt ccaacacgcc ttcgggcgag ttgacgttca agcgatgcgt
     5821 caaaccggcg gcgacgagat cgcaatcgat cagcaaggtt cgagcgccgc acgcagcgta
     5881 gctcaggccc agcgccagcg acaggctggt cttgccatcg ccgggggcgg cgctggtcac
     5941 cgcgatgacc tgcggttcat cattggtccg gctgatctgc agcatcgtgc ggatctggtg
     6001 cacgcaatgg gcggcgatgc cggcctgctg cgggtcgctc aagcggtccg gcaggttcgg
     6061 caggatgccg agcagcgtga cgccggccat gtccgattca ccggtttcat cgctatagcg
     6121 gaagcgggcg tcggtcatgc cgtacagcag cagcagcccg acggggatca atccgccgaa
     6181 catgaacccg gcgccggccg cgatcacgcg cttcttgtcg ccggtgttga ttgcttcgct
     6241 gccgtagtcg ttcacgtgca cgctctgcgc tagcttctgt tcggcttcga gcgattggat
     6301 ccggccttcg acgaccttca acccctcttc gttcttcgag atgtcgcgct gcttttcagc
     6361 gattctatta cgcagctcgc cgatcagcag gacctgcttt ttcttgtcgt cgaggcggga
     6421 ctgaagttga tcgatcttcg tcttcatcat cgccaggttc ccaccaccgt tggccggatc
     6481 gtccaagcca aacgggatct gcccggcgtg ggcgttcagg taatcgcggg cgtactgctc
     6541 caccgcgcgg ttcgcctgct ggtatttcag atactcagcg gcgtaaagcg gatgcttgcg
     6601 cccgagcttg aacaccagct gctccagtga atcgacggtg tgcccgcgct cggctagcag
     6661 ttgccgcatc tgtagatcga caacggcgat ctgcttgtag atgaactcgc gatcgttcgg
     6721 gttggtcgtt ggcttcttgc tttgcagatc cgtggccgcc ttgtactgca gtgtcgcctg
     6781 atccaactcc gcttccagct tcaattcctc gccctgcatc gcatcgaaca gcgggccgag
     6841 gtccgtcgtg ccatacgact ggctccaagc aaccaattgg ctgcgggcaa aactgagatc
     6901 gctgttcagc cggcgctgtt catctgtgtt gaacttgatc ttgatcgtcc gcgcattgct
     6961 gtcgccgtcg ccatacagct tcatgtatgc gcggatagcg gcgtgggcgc cggcttgtgc
     7021 gacttcctta tttttatcag cgaaggaaac cttgatgatg ttgccgtcct tctgccggtc
     7081 ggtcgtcagc gcggtgccga aatccgtcgc gctcatggcg ctggggctgc cgtagacttt
     7141 cgtcagcgcg ttcatgagtt ccggatcctt cagcgcttcc tcggccaccg aggtgctttc
     7201 gatcagcgcc gcctggctgc gcatcaccat ggtgaacagc gggaccgatt catcgtgcag
     7261 cccgggcgtc atgatcatcg ggttcacgct gatcatgccg tcgctctgcc acttgggcgg
     7321 gagcagggta tacagggcaa tgcacccggc gatcgcgccg atgccggcca tcaggaacgc
     7381 cagcggataa cgcccgcgca gcaagcggtg cgcgcggcgc agcggcgacg tgcgcggcgc
     7441 actgctgccg ccgccgccgc tgttcacggg accaccgacg gctgcctggc cttgcgtcca
     7501 gtagtccgcg gcggcggggt aattgttgct cagctggatt tccttgctca tcgaatacct
     7561 ccggacatca acgtcttgat gacaggcacg cacggctgca tcagcgttgt gaagatctca
     7621 tcgcgttcct gctgcggcag gtccgcattc atcaacagtt ggaactgggc ggcgccgtaa
     7681 tagcggcgct ggtaatcctc ggcggccttg cgcacgtcct tcatgtcgcg aaggatgccc
     7741 cggccgggca cgatcagaaa attgtagacc gccgtgcgcg tcgattcggt cgggctggtc
     7801 ttgacgaaat gatattcctt gcccgtgatc agcaggtcgc cgacccgcca gtcgcgctcc
     7861 tgcgagaaca tcagctcttc gccgttgttc acgtagcagt tgggcggcca gtggccgttc
     7921 atatcaccgg cgtcccggca ctgatcgacc agcaggctgg cccatcgatc gtaccagcgc
     7981 gggttgggcg cattgtcgct gtacttccac tcgcgaatga agttcggatg cagcaggcgg
     8041 atcgcctccg gggcgatctt ttcttcctgc ccggaccagg aaccaatctg catcgggatt
     8101 gattcaagcg cttcgcgagc gcgttcgtga tagggcccgg catcggcagg cgtgatacgc
     8161 gtgctgctct cggccaccat gccaagcagc aatgccaggc aggcggcggg agcggcgaag
     8221 atgacgcggg cgtttcgcat gggttcaggg ttcaggtttc aggaaagaag agacgccagt
     8281 aacggctttc tcgtttctga accctgaacc ctgctcactc aactcgctaa cgtatagcgc
     8341 attaccggaa tcatggccca gcgcagcagg cggatgatgc cgagcagcat taagagcgca
     8401 atcggcagca tcagccagcc gctgtagtcg tgaaacgcgg ttgccacggc ccggctggca
     8461 tttccgtaca gccagaccgt gggcaagatt cgaatcaggt tgcagaagat cgcagctatc
     8521 ggactaacca gcaggatcac gatgcggacc gaattccgca ggggcaggcc atagctgaac
     8581 gcgtagctca ccaggatcag cgcgaagacc atgcgaaggc cgttgcacgc ttcgatgatg
     8641 tgcaccggca cgccgttgat cgtcagttgg ttgccggaac gaacaacttc ggtgccgagc
     8701 acttcgaaca tggtctgctc gatccgcgcg gtccagttct gcagcggcag ggcgacgcgc
     8761 aggcggattt cgcctggcac gggcacgagg aacgccagca cggccaccac cggcaggaag
     8821 cgcaggatcg cgtgcttgcc cagcaccgcg acgatgcacc ccagcacgac gatgacggcc
     8881 ccgccgtgcc agaacgattg gatgccgtga tagaagccga atgagctgat cgcccagccg
     8941 acgccgacga ggatcgggcc gaggatcgtg ccggtcggtt tgcaatatcg aaagcgcacg
     9001 cggcgggccc acagcatcca gagcgcaacg acggggacca ggaagacgtg gctcgactcc
     9061 tcgtccctgc tggcgatgtg atagatgttc gcccacgctg gccaagtgac ggcgacgccg
     9121 atcgccgcca tgacggtggc ggcgaagacg tgtgcgaggg tccatcgatc agaacggaat
     9181 agacgcgacg tcatgcagcc ttgcctttgt cggccgggtc aagcgcagcg gcgggcgtgg
     9241 tcgcggtccc actgagatgc gggccgctgg cccgggggct gttcaccggg cggacgaaat
     9301 catcgacgac cgtctggtcc gggcgaacga gcccgctggg acagacgacc gatcgcacga
     9361 gcacagcgcc cgcttcgacg atcccaccgc gaagcacgac cgagtcgtgc acgcgggcgg
     9421 ttgaatcaac cctggcgcct tcttcaacga tgccgaacgc cggctgccag tcctcggcca
     9481 gcgggtccgt cagcgtggtc ttgccggcgc ggcgcatgtg ataaaagcgc attgccgtga
     9541 tgtagtcggt gacggagcga atcggcagcc cggtcggccg ccggcggtgc accacctcga
     9601 tcgcatattg ctgggcgatc gtcggcatcg cctgctcctt catgtcgatg aaaccagtct
     9661 tggggatgag ccggagcgtt ttgcagcgga cgagcatcat cccgctgggg gtgccgtcgc
     9721 ggtgcgagat gatcgcgacg tcgccgcggc gctggtccag gcttgccgcc agggaaacca
     9781 gcggatcgag caggatctgg gcggcgttgg cgacgaggat caagtcatca tcggcgtagt
     9841 tctcggccag gtcgcgcagc acaccgccgg tgccgcgata ttcggattga tcgcgttcga
     9901 cgcggaaggt gccgaaatat ttgacgcttg cgctgatcgg ctcgggtgag ttgcggtcca
     9961 ccatcacgcg caccggcagc ttttccaggc cggcgacctt cgcgagttcg ctcgcgtgcg
    10021 ccagccaatg gttcaacacc gacccctctt cgtcgagcgg caggtcgagc agcgatcgac
    10081 cgatcgacgt gctgagcggc gtcggtcgca cgtgtccgcc gagcaggatg atcgctttga
    10141 ggcgcgacag cgtggacacg gattgatcga gcgtcgtcgc catccggcgg gcgcgatcgg
    10201 tgaggcggtc gttcaccgag tcgccggcga cagtcgtcgc ggtgttcgcc gcaaccgccg
    10261 gcgcggtggg tgacgtcggg ctttcgacgc gcgcgaacga ggttgggaac gaagccggcg
    10321 ctggttgggt ccaacccaat cgcttgggaa tgccgcggac gatcgcgcgg gacatcaagc
    10381 tgctggcgaa cgggatgccg accacgatgt agcggtggaa caaccgacgc ggttcctggc
    10441 agaggcggtg cagccattcg atgccgcaca cctgcatcca gcgcggggcg cgcttcacat
    10501 gaccggtgag aaagctgaag ctgacgccca ctcccagcca ccacgcggtc tccatggtgg
    10561 atcgcaggcg cgcgatcacg tgttcctgct tcggcgagcc cagtgcgacg aagatgatgt
    10621 cgggcttggc gtcggtgata agctgcgcga tctccgccat ctgcttgggg ctgtcttcaa
    10681 accccaaccg cgggcagaac gcgccggcga ttttcagttc cggatggcgc tggcgaagaa
    10741 ttgccccggc gccttcagcg gtgccttcag cgccgccgag caggaagatc gacttggctc
    10801 gtcccgcggc ggcagcgctg agcgacgaga tcagattcga tccggcgacc cgctcgggca
    10861 gcggggtgcc ttgcaagcgg ctggcccaga ccagcggcat gccgtcggcg acgaccaagt
    10921 ccgcctcggc gacgagcgcg ccgaagttca ggtcgcgcac gcatcgccgc aggtgatcaa
    10981 ggttcggggt gacgaccaca ccgccgcggc gggcgtccag ctcatcgaga atatgttcga
    11041 tgcactcgcg ctcggtgact cggtgcagct tcaccccgtg caggtgaatg aacggcagca
    11101 acttcccgtt ttgaccgacg gtgccaccgt tgcgcttgta cccatcccga cgggacgggc
    11161 gcgcgaccag cgccggcgcc gccttggcgg tagccaaatc gctggcgtct gctgcatccc
    11221 aggaattgtc cccatctcga aagtacatgg acctcagctt ccctg
//
LOCUS       DATIMH010000466         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3572167, whole genome shotgun sequence.
ACCESSION   DATIMH010000466 DATIMH010000000
VERSION     DATIMH010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3572167"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VLI90_08055"
     CDS             <1..203
                     /locus_tag="VLI90_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14198.1"
                     /translation="ETILGLGAGDLEAMDGSGKVVKRWPNILFLAFTWSSLDEILHQR
                     AATVDNPTNDPVEVEQVRREKA"
     gene            233..490
                     /locus_tag="VLI90_08060"
     CDS             233..490
                     /locus_tag="VLI90_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14199.1"
                     /translation="MNDADRILDYGTRGPRRSPVPMFGIAVCGLGVFSGILIAGFGVW
                     WFIATGADGAARTVGIIFTGALLIYLCARWYHKARSAMSAA"
     gene            511..960
                     /locus_tag="VLI90_08065"
     CDS             511..960
                     /locus_tag="VLI90_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14200.1"
                     /translation="MGFFDRLLGKKAVQPIDPDAPFMEHPTGDFKSAVDAMADAIQRL
                     RALPQWDEWINFCGQGVGHDEDSDHLAEIRVRRDELSVDEPVDTESVIRKAGVPPSAL
                     VAAGGNFSIAAATPAEAARILDAIFRLCLGIRPHADEGDDYAVGAEW"
     gene            1172..1411
                     /locus_tag="VLI90_08070"
     CDS             1172..1411
                     /locus_tag="VLI90_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14201.1"
                     /translation="MKRLIPGRKELALFATLAIVLSSIVSMFQVSQHDLWLAALAMPM
                     FVFAAVLGLRRSPQWQPLPEEVAAAVETSNEQVSA"
     gene            complement(1701..>2988)
                     /locus_tag="VLI90_08075"
     CDS             complement(1701..>2988)
                     /locus_tag="VLI90_08075"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV14202.1"
                     /translation="QYMATAVADYVRNGFTVDTAAPTVTLMNVPNVSGANAAAPYQFT
                     VTYNDNFFLNTSTFDSNDLLVTGPNGFSQKATFVSSGLVWTTSDGHDNRVVTYQIQPP
                     GGAWSSGSDGAYTISLLANQVLDSAGNAAAGGMLTQFTVDTSAPAGSLLAAPNVDSTS
                     LGSTTYTFAVSYSDASGIDLSSLDGNDIEVTSTTGYDALATLNGTSVNGNSVIATYSI
                     AAPSGAWSTDAAGTYNIIQRAGEVRDTFGNTAPAGTLGTFTASFGGGPNPPPASGAIS
                     GTVFNDANGNGTIDSRELPMVGVVVYLDTNLNGQLDANEATATTDDTGQYTFNGLSDG
                     RYTVREQSPGGYTVTSPTSGTNTATITGGTPVSGVSFANQLSSLVTGTPSATPSSPST
                     PTTSPTSPTTTPTSGTPTDTSKPRPKNPGRGHTVKL"
BASE COUNT          494 a          876 c         1016 g          602 t
ORIGIN      
        1 tcgagacgat ccttggcctc ggcgccggcg atctggaagc gatggacggc agcgggaagg
       61 tagtcaagcg ctggccgaac atcctgttcc tggcgttcac gtggagttcg ctggacgaga
      121 tcctgcacca gcgcgcggca accgttgaca atccgacgaa cgaccccgtc gaggtggaac
      181 aggtccggcg ggagaaggca taagaatacg tttgattgcc ggaggcgcgg gcgtgaacga
      241 tgcagatcgc atcctcgact atggcacgcg cggaccgcga cgatcgccgg tgccaatgtt
      301 cggcattgcg gtctgcggcc tcggcgtgtt cagcgggatc ctgatcgcgg gctttggagt
      361 ctggtggttc atcgcgaccg gcgcggacgg cgcggcccga accgtgggga tcatcttcac
      421 cggcgcgctg ctgatctacc tttgcgccag gtggtaccac aaagcgcgat cggcaatgtc
      481 cgcggcgtaa caaaaacctt cggaggcgcc atgggattct tcgatcgctt gctgggaaag
      541 aaggctgtgc aaccgatcga cccggatgcg ccgttcatgg agcatccgac cggcgacttc
      601 aaatccgccg tggacgcgat ggcggatgcc atccagcggc tgcgggcatt gccgcagtgg
      661 gatgagtgga tcaacttctg cggccaaggc gtgggacatg atgaggacag cgatcacctg
      721 gcggagatcc gcgtgcggcg ggacgaactt tcggttgacg agcccgtaga taccgagtca
      781 gtcatccgaa aagccggcgt gccgccatcc gcgcttgtcg cggctggggg gaacttctcg
      841 atcgctgccg cgacacctgc agaggccgct cggattctcg acgcgatctt tcggctctgc
      901 ctgggcattc gcccacacgc cgacgaaggc gacgattacg ccgtcggcgc tgagtggtga
      961 acgcgaagcg aaaaatcggc tgaaaatacc ggcatcagtc gcttgaatcc gggtcgccga
     1021 gtgatagatt cgcaccagcg gtgtccgctt cggggctgtt ggcgaacgcc gcgtcacgtt
     1081 tcaagcaatg tttgcgtcca gccggcgacg gtcgcgggat tcgcggcttt aaggtcgctg
     1141 cggttttagg gcttactgtg ggacatcctt tatgaagcgc ttgatcccgg gccggaagga
     1201 attggctctg ttcgccaccc ttgcgattgt gctgtcgtcg atcgtgtcga tgttccaagt
     1261 gtcgcagcac gacctgtggc tggcggcgct ggcgatgccg atgttcgtct tcgcggccgt
     1321 gctgggcctg cgtcgcagcc cgcagtggca gccgctacct gaagaggtcg ccgccgcggt
     1381 ggaaacatcg aacgagcaag tctcggctta gcatcttccg tttaaattcg atccagtcgc
     1441 acgcagttgt tgtgcggccg cgcgttgacg cggcgcgttt tccggggaag gcgcgccgcg
     1501 ttcggcgttt gtgggcgcgc gtgcgcgctt ggtcgagacg agcgcgtcac agccgcttgc
     1561 ggcttagcat gttttctctt gccgaacaga cgctgggcgc aagcggcagc cattgcgcaa
     1621 gtggcagtac actcatttcc agtcaggatc cttcgactgg gcacgagtac gtggtccgct
     1681 caggctgaag attcaatcgc ctacaactta acggtgtgcc cacggccggg attcttcggc
     1741 ctgggtttgg acgtgtccgt gggcgtgccg ctcgtcggcg tggtggttgg cgacgtcggt
     1801 gatgtcgtcg gcgtacttgg tgaactcggc gtagcgctcg gtgttcccgt taccaatgaa
     1861 ctgagctgat tcgcgaaact caccccgctg accggtgtgc cgccggtgat cgtcgcggtg
     1921 ttggtgccgc tcgtcgggct cgtcacggtg taaccgccgg gcgattgctc gcgcacggtg
     1981 tatcggccgt cgctcaggcc gttgaacgtg tattgcccgg tgtcatccgt cgtggcggtg
     2041 gcttcgttgg catcgagctg gccgttcagg ttcgtgtcga gatagacgac gacgccgacc
     2101 atcggcagct cgcgcgagtc gatcgtgccg ttgccgttgg cgtcgttgaa gacggtgccg
     2161 ctaatcgcgc cgctggccgg cggggggttg ggcccgccgc cgaaggacgc ggtgaacgtg
     2221 ccgagcgtgc ccgcgggcgc ggtgttgccg aaggtgtccc gcacttcgcc ggcgcgctgg
     2281 atgatgttgt aggttcccgc ggcgtcggtg gaccaggcgc cgctgggcgc ggcgatcgaa
     2341 taggtcgcga tcacgctgtt gccgttcacg ctcgtgccat tcagcgttgc cagcgcgtca
     2401 tatcccgtgg tactggtcac ctcgatgtca tttccgtcca gcgatgacag gtcgatgcct
     2461 gaggcatcgg agtaactcac ggcgaacgtg taggtggtcg atccgagcga cgtgctatcg
     2521 acgttcggcg ccgcgagcaa cgaaccggcg ggcgcggagg tgtcaaccgt gaactgagtc
     2581 aacattccgc cggctgccgc attgccggcg ctatcgagca cctggttggc cagcagcgag
     2641 atggtgtacg cgccgtcgct gccgctcgac cacgcgccgc cggggggttg gatctggtac
     2701 gtcacgaccc ggttgtcatg accatcggac gttgtccaga ctaaacccga agaaacgaac
     2761 gtcgccttct gcgaaaaacc attcggcccc gtcacgagca gatcattcga atcgaacgtc
     2821 gaggtgttca ggaaaaagtt atcgttatac gtgacggtga actgatacgg cgccgccgcg
     2881 ttcgcgccgc tcacgtttgg cacgttcatc agcgtgacgg tgggcgcggc agtatcgacg
     2941 gtgaatccat tccggacgta atctgccacc gcggtggcca tgtactgc
//
LOCUS       DATIMH010000467         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4438035, whole genome shotgun sequence.
ACCESSION   DATIMH010000467 DATIMH010000000
VERSION     DATIMH010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4438035"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /gene="hisG"
                     /locus_tag="VLI90_08080"
     CDS             complement(<1..835)
                     /gene="hisG"
                     /locus_tag="VLI90_08080"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HSV14203.1"
                     /translation="MKLKLGIPKGSLENATIDLFRRAGFSITTSSRSYFPGIDDPEIE
                     CMLIRAQEMARYVEDGILDAGLTGRDWIQENEADVVAVADLIYAKQSFGKVRWVLAVP
                     EASGFHTAKDLQGKIIATELVQTTKRYLASHGVTAKVEFSWGATEVKPPVLADAIVEV
                     TETGSSLRANKLKIIDTVLESNTQLIANKSSWQDDQKRRKLEDIKMLLDGAINALGKV
                     GLMLNVHRDNLAAVLSVLPALKRPTISNLSEEDWLAVNTILDESTVRNIIPRLKEAGG
                     EG"
     gene            complement(832..1212)
                     /gene="hisI"
                     /locus_tag="VLI90_08085"
     CDS             complement(832..1212)
                     /gene="hisI"
                     /locus_tag="VLI90_08085"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HSV14204.1"
                     /translation="MNLDFAKSDGLVTAVVQDATSGRVLMVGYMNEESFRKTVETGFV
                     TFYSRSRKKLWMKGESSGHRLVVKEISTDCDLDAVLVKVDAQGPGVCHEGYQSCFFRR
                     LENGAWQVCDERTYDPNAVYGGKA"
     gene            complement(1451..>2583)
                     /locus_tag="VLI90_08090"
     CDS             complement(1451..>2583)
                     /locus_tag="VLI90_08090"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HSV14205.1"
                     /translation="IWNVVLVSSSFFLCIFGTMMTRSGLVDSVHAFAKSSIGNWFTAF
                     LAVGIAATVWLILSRLDYLRGDARMENVVSRESSFMLNNLLLLASCIAILWGTLFPVI
                     SQFVSGDKVTLEKPWFNRIMTPLGLALLLLMGVAPLLPWRRASLETVRRTFRIPGLGA
                     LVIAAILWSQNVPFGYALVSFSLCAFVTLTIAMEFFRGARAIATKEAVAFPRALVLLT
                     RRNTRRYGGYIVHLAVVLIFIGLTGAAFNRETKAEIRIGDRLTVGAYTLNVRSIDTGE
                     DANLLWQSALVDVYQDGRALGSMTPRREMYKASRQSVGRVDIRHGLNEDLYVNFAGLN
                     SSDMPVVEAYLFPLVNWLWIGAIVLLVGTCITLAPQTISVTG"
BASE COUNT          508 a          745 c          832 g          498 t
ORIGIN      
        1 tgccttcgcc acccgcctct ttcagtctgg ggatgatgtt ccgcacggtg gactcatcca
       61 ggatggtgtt gacggccagc cagtcttcct cgctgaggtt ggagatggtg ggccgcttga
      121 gtgccggcag cacggagagg acggcggcca ggttgtcccg gtgtacgttg agcatcagcc
      181 cgaccttgcc gagcgcgttg atggcgccat cgagcagcat cttgatgtct tccagtttgc
      241 ggcgcttctg gtcgtcctgc caggagcttt tgttggcgat caactgcgtg ttggattcga
      301 gcaccgtgtc gatgatcttg agcttgttgg cgcggagcga cgagccggtt tcggtgacct
      361 cgacgatcgc gtctgccagc accggcggct tgacttcggt cgcgccccaa ctgaattcga
      421 ccttggcggt gacgccgtgc gaggcgagat acctcttagt tgtttgcacg agttcggtgg
      481 cgatgatctt gccctgcagg tccttggcgg tgtgaaagcc cgacgcctca gggacggcga
      541 gcacccagcg aaccttgccg aaactctgct tcgcatagat caggtcggcg acggcgacca
      601 cgtcggcttc gttctcctgg atccaatcgc ggccggtgag tccggcgtcg agaatgccat
      661 cctcgacgta gcgcgccatt tcctgcgcgc ggatcagcat gcactcgatt tcggggtcgt
      721 cgatgccggg gaagtaggag cggctgctgg tggtgatcga gaagccggcg cggcggaata
      781 ggtcgatagt ggcgttctcc aggctgcctt ttgggatgcc gagtttgagc ttcatgcctt
      841 gcctccgtaa acggcgttag ggtcgtaagt gcgttcgtcg cagacctgcc acgcgccgtt
      901 ttccaggcgg cggaagaagc agctctggta gccctcgtgg cagacgccgg ggccctgcgc
      961 gtcgaccttc accaggacgg cgtcgaggtc gcaatcggtc gagatttcct tgaccacgag
     1021 gcgatggccg gagctttcgc ccttcatcca cagcttcttg cgcgagcggc tataaaatgt
     1081 gacgaagccg gtctccacgg ttttgcggaa cgactcttcg ttcatgtagc cgaccatgag
     1141 gacgcggccg gatgtggcat cctgcacgac ggcggtgacc aggccgtccg acttggcgaa
     1201 atcgagattc atacgcgtaa aaaacaaaaa gcccgccgtt tttgcggcgg gcgttccttt
     1261 ccaatttcta tttggagtga atcagaacat ggcccgtcgc gggtggctgt ggtgatgatg
     1321 atgccgcgcg acggtgatag ctaccataaa ccagcagtat aacacgagga aatacgcgag
     1381 gaaatacggt gacaaatacg gcaaatacgg tgacaggcta cgctttcact taatttaatt
     1441 gggtggaagc ctagcctgtc accgaaattg tctggggcgc caacgtgatg cacgtgccga
     1501 ccagcaggac gatggcgccg atccacagcc agttcacgag cgggaataag tacgcctcga
     1561 ccaccggcat atcgctggaa ttcaggccgg cgaaattcac gtagaggtct tcgttgagcc
     1621 cgtgccggat gtcgacgcgg cccaccgact ggcggctggc cttgtacatc tcgcgcctgg
     1681 gcgtcatgct ccccagggcg cggccgtcct ggtacacgtc gaccagggcg ctctgccaga
     1741 gcagatttgc gtcttcgccg gtatcgatgc tgcgcacgtt gagcgtgtac gcgcccaccg
     1801 tgaggcggtc gccgatgcgg atctcagctt tagtctcgcg attgaaagca gcgcccgtga
     1861 gtccgatgaa gatcaggacg acggcgaggt gcacgatata tccgccgtaa cgtcgcgtgt
     1921 tgcggcgggt gagcagaacg agcgcgcggg gaaaagccac cgcttccttt gtggcgatcg
     1981 cgcgcgcgcc gcggaagaac tccattgcga tagtgagggt cacgaaggcg cagaggctga
     2041 aagagaccag cgcatagcca aagggcacat tctgcgacca gaggatcgcc gcgatcacca
     2101 gcgcgcccag tcccggaatg cgaaacgtgc ggcgcacggt ttccagcgat gcgcggcgcc
     2161 acggcagcag cggcgccacg cccatcagca gcaagagcgc cagtccgagc ggcgtcatga
     2221 tgcgattgaa ccagggcttc tccagcgtga ccttatcgcc gcttacgaat tgggagatca
     2281 ccgggaacag cgtgccccag aggatggcga tgcaacttgc cagcagcagc aagttattca
     2341 gcatgaagct ggactcgcga gagaccacgt tctccatgcg cgcgtcgccg cgaagataat
     2401 ccagccgcga gagaatcagc cagacggtgg ccgcgatgcc tacggcgagg aaggcagtga
     2461 accagttgcc gatcgacgat ttggcgaagg cgtgtaccga atcgaccagt cccgagcgcg
     2521 tcatcatcgt gccgaaaatg cagaggaaga acgacgagga gaccagcacg acattccaga
     2581 tgc
//
LOCUS       DATIMH010000468         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2416116, whole genome shotgun sequence.
ACCESSION   DATIMH010000468 DATIMH010000000
VERSION     DATIMH010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416116"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2073
                     /locus_tag="VLI90_08095"
     CDS             <1..2073
                     /locus_tag="VLI90_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14206.1"
                     /translation="RARARDGPGGDMTTAPPPAPTPAVPKESADREVRQYRELLATPD
                     RWEGGFDVKSIVGGLFVGMIMTPASMYMQLVVGSEIGTAAQWVTIILFMEVARRAFTV
                     LTRPEIFVLYYMAGACLVNTGPGKGLLWQQFMVQSESFRQFGLADKIPAWIAPSSPDV
                     LGLRSFFNPGWYAPIGLIILGHILARVDNFGLGYVLYRLTSDVEKLPYPMAPVGAQGV
                     TALADASRGEETWRWRVFSFGAMLGIAFAAVYLALPSITGAFLPEPISMIAFPFKDLT
                     SNTEDFLPAVPIIIALDLGLLISGMVLPFWAMAGSFIGLVACMVLNPVLYHYGILSSW
                     VPGLGAVRTLQSNTLDFYFSFGLGLTAAIAVVGIWHVASRLIVKREGRGQTNWRALFT
                     PPPGRGDFSVWIALFLYFAVTASTIILAYMLLRQSHLAGIGSPVTMTLLGIFAFYGFV
                     YTPIIGYVSARMEGVVGQNVQLPLVREAALLMTGYQGAAIWFVPFPANNHGNQALYFR
                     KTELTGTKITSMIKAELFILPVILIATVVFSHLIWRIAPVPSSAFPYAEQWWELSAYK
                     QGLIYSSTLPGGGGSHFAQAFHGRYLFAGLGGALLLYSGLASFGLPVMLFYGIIRGLD
                     QSAPHVILPQFIGALLGRKYFQKKFGERWKPYIIVFFAGYSCGVGLIMMFSLGCVFMS
                     KSVFQRMY"
     gene            2081..>2822
                     /locus_tag="VLI90_08100"
     CDS             2081..>2822
                     /locus_tag="VLI90_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV14207.1"
                     /translation="MSESTTSPSSQSPPVQGLAELSVRTIDVKRSYKMGSETVQALRG
                     VSISIDRGEYISIMGPSGSGKSTLFNMIGGLDKPSEGKVYIDEVDIAQLDSYELAWMR
                     CHKIGYIFQTFNLIPVMTALENVMMPMVFAGVNTADAQDKAAAILKRVELGHRMTNRP
                     SEMSGGQQQRVAIARAFANDPTIILADEPTGNLDLHTGRLIIELLRQLNQEREVTIIS
                     ATHDHKMLSVSDRILWISDGRVERIQKRE"
BASE COUNT          494 a          891 c          902 g          535 t
ORIGIN      
        1 cgcgcccgag cccgagacgg tccggggggc gacatgacca ccgcgccgcc gcccgcgccc
       61 acgccggccg tgccgaagga atcggccgac cgcgaggtcc ggcagtaccg cgaactgctc
      121 gcgacgccgg accgctggga aggcgggttc gacgtcaagt cgatcgtcgg cgggctcttc
      181 gtcggcatga tcatgacgcc ggccagcatg tacatgcagc tggtcgtcgg gtccgaaatc
      241 ggcacggccg cacaatgggt cacgatcatc ctgttcatgg aagtcgcccg gcgcgcgttc
      301 accgtgctga cccggccgga aatctttgtg ctgtactaca tggccggcgc ctgcctggtg
      361 aacacgggcc ccggcaaggg gctgctctgg cagcagttca tggtgcagag cgaatcgttc
      421 cggcagtttg gattggccga caagattcca gcgtggatcg ccccgtcgtc gccggatgtg
      481 ctggggctgc gcagcttttt caaccccggt tggtacgcgc cgatcgggtt gatcatcttg
      541 ggtcacatcc tggcccgcgt cgataatttc ggcctgggtt acgtgctgta tcgcctgacc
      601 agcgacgtgg aaaagctgcc gtacccgatg gcgcccgtcg gcgcgcaggg cgtgaccgcg
      661 ctggccgatg cgtcgcgcgg cgaagagacc tggcgatggc gcgtcttcag cttcggcgcg
      721 atgctcggca tcgccttcgc cgcggtttat ttggcgctgc cgtcgatcac cggcgcgttt
      781 ctcccggagc cgatcagcat gatcgccttt ccgtttaagg acctgacgag caacaccgag
      841 gacttcctgc ccgcggtgcc gatcatcatc gcgctcgacc tgggtctgct catcagcggg
      901 atggtgctgc cgttctgggc gatggcgggc agcttcatcg ggcttgtcgc gtgcatggtg
      961 ctgaacccgg tgctgtacca ctacggcatt ttaagcagct gggtgccggg gctcggcgcg
     1021 gtgcgcacgc tgcagtcgaa cacgttggat ttttatttct cgttcggact cggactgacc
     1081 gcggccatcg cggtggtcgg catctggcac gtggccagcc gattgatcgt caagcgcgaa
     1141 ggccgcgggc agacgaactg gcgcgcactc ttcacaccac caccaggtcg aggcgatttc
     1201 tccgtctgga tcgcgctgtt cctgtacttc gccgtgacgg cttcgacgat catcctcgcc
     1261 tacatgctgc tgcggcagtc gcacctggcg ggcatcggca gtcccgtcac catgacgttg
     1321 ctcggcatct tcgcgtttta cgggttcgtc tacacgccga tcatcggtta cgtcagcgcc
     1381 cgcatggaag gcgttgttgg ccagaacgtg cagctgccgc tggtccgtga agcggcgctg
     1441 ttgatgacgg gatatcaagg cgcggcgatc tggttcgtgc cgttccccgc gaacaatcat
     1501 ggcaaccagg cgctctactt tcgcaagacc gaactgaccg gcacgaagat cacgtcgatg
     1561 atcaaggccg agctgttcat cctgccggtc atcctcatcg ccaccgtcgt cttcagccat
     1621 ctgatctggc ggatcgcgcc ggtgccgagc agcgcctttc cgtacgccga gcaatggtgg
     1681 gaactgagcg cgtacaagca gggattgatc tacagcagca cgctgcccgg cggtgggggg
     1741 agccacttcg cgcaggcatt tcacggccga tatctgttcg ccgggctggg cggcgcgctg
     1801 ctgctttact cgggcctggc gtcattcggt ctgcccgtga tgctcttcta cggcatcatc
     1861 cgcgggctcg atcaaagcgc gccgcacgtg atcctgccgc agttcatcgg cgcgctgctg
     1921 gggcgaaaat atttccagaa gaagttcggc gagcgctgga agccgtacat catcgtcttc
     1981 ttcgccgggt acagctgcgg cgtggggctg atcatgatgt tcagcctcgg ctgcgtgttc
     2041 atgagcaaga gcgtctttca gcggatgtat tgagatggac gtgagcgaat ccacaacatc
     2101 gccatcatca cagtcgccgc cggtgcaagg gctggccgag ctaagcgtgc gcacgatcga
     2161 cgtgaagcgc agctacaaga tgggcagcga aaccgtgcag gcgcttcgcg gcgtgagcat
     2221 ctcgatcgac cgcggtgaat acatctccat catgggcccc agcggttccg gcaaaagcac
     2281 gctgttcaac atgatcggcg gcctggacaa gcccagcgaa ggcaaggtct acatcgacga
     2341 agtcgatatc gcgcagctcg acagctacga gctggcgtgg atgcgctgcc acaaaatcgg
     2401 ctacatcttt cagacgttca atctgatccc ggtgatgacc gcgctggaga acgtgatgat
     2461 gccgatggtc ttcgccggcg tgaacaccgc cgacgcccag gacaaagccg cggccatcct
     2521 caagcgcgtc gagctgggtc accgcatgac caaccgcccc agcgaaatgt ccggcgggca
     2581 gcagcagcgc gtcgccatcg cccgcgcgtt cgccaatgac ccgacgatca tcctggccga
     2641 cgaaccgacg ggaaacctcg acctgcacac aggccggttg atcatcgagc tgctgcgcca
     2701 gctcaaccag gagcgggagg tgacgatcat cagcgcaacc cacgatcaca agatgttgtc
     2761 ggtcagcgac cggatcctct ggatcagcga cgggcgcgtc gagcggatcc agaagcgcga
     2821 gg
//
LOCUS       DATIMH010000469         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3284041, whole genome shotgun sequence.
ACCESSION   DATIMH010000469 DATIMH010000000
VERSION     DATIMH010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3284041"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3365)
                     /locus_tag="VLI90_08105"
     CDS             complement(<1..>3365)
                     /locus_tag="VLI90_08105"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HSV14208.1"
                     /translation="QGTNTISKYTATGRLSNVGIENIGGISQYDPTVTDPTDGPVDEN
                     HPWIFINVDGAINSWVRNVTSQFFGYACVDVTNSKFVTVQDSQCLDPISQITGGRRYS
                     FTIDGSQDTLVRNCYTREGRHDYVEGSTVTGPNVFVDDRADAEHADAGPHHRYSTAAL
                     WDNLTVGQLDIQNRGNSGTGHGWAGANQVIWNSKANSFIVQNPPAAQNWLIGSVGAIA
                     NGTMYVGPHDPGIYDSSGSSGVNVAQRSLYYQQLSERTAYENFALRESRLGDNDGFVT
                     GDAADAVPVDATWKSSIQTATGKTGVEFDAASDGQLVPFTFNFNLDPNTYIVGASLSL
                     GLKSTGGGTSGDAIYIEDKSRQYTWAQLGVAAPTTTEAGVVIDLSKLIPALQDGRLNL
                     AILGNTTLDWATLNFQTASTTQPTATTLTASEDATVQDGSSANSNFANATTLQTKLDT
                     VNNDQEAFLKWDLSSLSASGFITRATIRLVPTTVGYAPDTFNSGKGAIFNQLSFVSDD
                     TWTEGGITWNNKPAAGAMVAEFISKANAPINVDVTALVRAALAGDRKLSLKLLSIVQD
                     SAGQISYASSENGTASLRPQLIISTYGSALTPVADAYVRDGSSANTNFGSATDLETKK
                     DSAANSGNNRDGYLKFDLTSIATAPAGATVRLMPIQVGAQLPETADFVTTDSWTESGI
                     TWNNKPANTTALGSFNVWQATPVKFSVGPQVASTIAGDKLLSVHLGTATANSGVNFFA
                     SREYADGSTVPLLILKNLGPQITSIQNQSGGVNQLVSSEWFGIWDAETAAGSLAVSAI
                     SSNTTLLPNANITFSGTTGDRFFTLTPASGQTGSTNVTITVTDAQGQTATSVFSYIVS
                     STLAINGDQDSAGENDVFRVVRNGTFVDIYRNNTVTPAFHMDYASAPQLLINTLGGND
                     IVTVDYSGGNPVPAAGIIVDGGTGNDTVAASGGAGADAVTLATGTVSVNGGQFSYANS
                     EELDLGAGTGGDTIAATGNASVSTSLFTVTVTGGTLSMAASSTLPDFTYMVVSSGATF
                     SLNGQNETVDALTGTGTVNDNNATAATLSVGQGGSSFAFAGVLANGGTGTFSLAKVGA
                     GSFTLSGANTYMGTTTITAGEIASGISASFAGLA"
BASE COUNT          523 a         1074 c         1075 g          693 t
ORIGIN      
        1 gcgagccccg cgaagctggc ggagatgccg ctggcgattt cccccgcggt gatcgtcgtc
       61 gtgcccatgt atgtatttgc gccgctcagc gtgaacgatc cggcgccgac cttggccagc
      121 gagaacgttc cggttccgcc gttcgccagc acacccgcga acgcgaagct gctgccgccc
      181 tggccgacgc tcagcgtcgc ggccgttgcg ttgttgtcgt tgaccgtgcc ggtgccggtt
      241 aatgcgtcga cggtttcatt ctgcccgttg aggctgaacg tcgccccgct gctcacgacc
      301 atgtaggtga agtccggcag cgtgctgctg gccgccatcg acagcgtccc gccggtgacg
      361 gtcaccgtga agaggctcgt ggagaccgac gcgttgcccg tcgccgcgat cgtatcaccg
      421 ccggtgccgg cgccgagatc cagctcttcg gagttcgcgt agctgaactg cccgccgttg
      481 accgagaccg ttccggtggc cagcgttacg gcatccgcgc ccgcgccacc gctggcggca
      541 accgtgtcgt tgccggtgcc gccatcgacg atgatgcccg ccgccggcac ggggtttccg
      601 ccgctgtagt caacggtgac gatgtcgttg ccgcccagcg tgttgatcag caactgcggc
      661 gcgctcgcgt agtccatgtg aaacgccggc gtcaccgtgt tgttgcgata gatatccacg
      721 aacgtgccgt tgcgcacgac gcggaagacg tcattctcac cggccgagtc ctggtcgccg
      781 ttgatcgcca gcgtgctgct gacgatgtac gagaagaccg atgtcgcggt ctgtccctgc
      841 gcgtcggtca cggtgatcgt gacgttggtc gatccggttt gcccgctggc gggcgtgagc
      901 gtgaagaaac gatctcccgt ggttccactg aaggtgatgt tcgcgttggg cagcagcgtc
      961 gtgttggatg agatcgcgct gacggccaat gatcccgcgg ccgtctcggc gtcccagatg
     1021 ccgaaccatt cgctcgacac tagttggttc acgccgcccg actggttctg aatcgacgtg
     1081 atctgcgggc cgaggttctt caggatcagc agcggaacgg tcgagccatc ggcatattcg
     1141 cgcgacgcga agaagttcac gccgctgttg gccgtcgcgg tgcccagatg caccgacagc
     1201 agcttgtccc ccgcaatggt gctggcgacc tgcggaccaa cgctgaattt caccggcgtg
     1261 gcctgccaca cattgaagct gcccagcgcg gtggtgttgg cgggtttgtt gttccacgtg
     1321 atgccggact cggtccaact gtcggtggtg acgaagtcag ccgtctccgg caactgcgcg
     1381 ccgacctgga tcggcatcaa gcgcaccgtc gcccccgccg gcgcggtggc gatgctggtg
     1441 aggtcgaact tcaaataccc atcgcgattg ttcccgctgt tggcggctga gtccttcttg
     1501 gtttccagat cggttgcgct gccgaagttg gtgttggcgc tgctgccgtc gcgcacgtag
     1561 gcgtcggcca ccggcgtgag cgcgctgccg taggtcgaga tgatcagctg cgggcgaagg
     1621 ctggcggtgc cgttttcgct ggatgcatag ctgatctgcc cggccgaatc ctgcacgatc
     1681 gacagcagct tcagcgacag cttcctgtcg cccgccagcg ctgcgcgaac gagcgcggtg
     1741 acatcgacgt tgatcggcgc gttggccttg gagatgaact ccgccaccat cgcccccgcg
     1801 gcgggcttgt tgttccacgt gatgccgcct tcggtccacg tgtcgtccga cacgaacgac
     1861 agctggttga agatcgcgcc cttgccggag ttgaatgtat ccggcgcata gccgaccgtc
     1921 gtcggcacca gccgaatcgt cgcgcgggtg atgaagccgc tggccgacag cgacgacagg
     1981 tcccacttca aaaatgcttc ctgatcgttg tttaccgtgt cgagcttcgt ctgcagcgtg
     2041 gtggcgttgg cgaagttgct attggcgctg ctgccgtcct gcaccgtcgc gtcttccgac
     2101 gccgtcagcg ttgtcgctgt cggctgcgtt gtcgaagcgg tttggaaatt cagcgtcgcc
     2161 cagtccaacg tggtgttgcc gaggatcgcc aggttcagcc gcccatcctg cagcgccggg
     2221 atcagcttcg acagatcgat caccacgccc gcttcagtcg tcgtcggggc ggccacgccc
     2281 agttgagccc aggtgtactg ccggctcttg tcttcgatgt aaatcgcgtc gccgctcgtc
     2341 ccgccgccgg tggatttcag gccaaggctc aggctcgcgc cgacgatgta cgtgttggga
     2401 tcgaggttga agttgaacgt gaacggcacc agctgcccat cgctcgcagc gtcaaactca
     2461 acgccggtct taccggtggc ggtttgaatg ctgctcttcc acgtcgcatc caccggcacg
     2521 gcgtcggcgg cgtcaccagt gacgaaacca tcgttgtcac cgaggcgaga ttcgcgcagc
     2581 gcgaagttct cgtacgccgt ccgctcgctt agctgctggt aatagagcga tcgctgcgcg
     2641 acattcaccc cgctggaacc ggacgaatcg tagatgcccg gatcgtgcgg gccgacgtac
     2701 atcgtgccgt ttgcgatcgc gccaacgctg ccgatcaacc agttctgtgc cgccggtggg
     2761 ttctgcacga tgaagctgtt ggccttggaa ttccagatga cctggttcgc ccctgcccag
     2821 ccgtgcccgg tgccgctgtt gccgcggttc tgaatgtcga gctggccgac cgtcaggttg
     2881 tcccacaatg ccgcggtgga atagcgatga tgcggcccgg catccgcgtg ctcggcatcg
     2941 gcgcgatcat ccacaaaaac attcggaccc gtcacggtcg aaccttcgac gtaatcgtgc
     3001 cggccctcgc gcgtgtagca gttgcggacc agcgtgtcct ggctgccgtc gatcgtgaac
     3061 gaataccgcc gcccgccggt gatctgcgaa atcggatcaa ggcattgcga atcctgcacc
     3121 gtcacaaatt tcgaattggt gacatccacg caggcgtagc cgaagaactg gctggtgacg
     3181 ttgcgcaccc acgagttgat cgcgccgtcc acgttgatga agatccacgg gtggttctcg
     3241 tccaccggtc cgtcggtcgg gtcggtcact gtcgggtcgt actgcgaaat gccgccgatg
     3301 ttttcgatgc cgacgttgct caaccggccg gtcgcggtgt acttcgagat cgtattcgtc
     3361 ccctg
//
LOCUS       DATIMH010000470         6199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_974463, whole genome shotgun sequence.
ACCESSION   DATIMH010000470 DATIMH010000000
VERSION     DATIMH010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974463"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..760
                     /locus_tag="VLI90_08110"
     CDS             59..760
                     /locus_tag="VLI90_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14209.1"
                     /translation="MTVTERLERRRLFSVTVTEDSPGFFEVNGDGGDNAINVSISQNN
                     GTFTLDNTTYSEVSYLYVNGNGGNDTISVTSSDGPGNIGASIVTLDGNDNITLGVSGG
                     VWAGGGDDTVNVSDSYWGDINGEAGNDKIYISGDTIDAQISGGSGNDLIDASNNNAPV
                     TIHGDAGNDSIYGSAFDDELYADSGNDSVCGNAGDDIVYARLSNGDTIDGGDGTDVLY
                     ASGSEVSVNYVEYVI"
     gene            825..1628
                     /locus_tag="VLI90_08115"
     CDS             825..1628
                     /locus_tag="VLI90_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV14210.1"
                     /translation="MNKRTIRSGGFTLTELLVVVGLIALLLALLMPVVGKARAAANST
                     GCLSNLRQMGIAWTMYAAENKGRLLENLKPTPLNPDIAWRGYWTGVLDGYQVRGDALL
                     CPAANRTIPFNQISGYGNVNFAWTGKYGPNATSVHYNATIYRSSSYGFNEYLAANGGF
                     DTDSLATNITKLRPLAEVPVFADSVWVDFAPRNFSAGFAPVQPPPNLRGEGFTLTSPD
                     HWRFLIARHGRAVNVCFADGSASRVPLEETYLLRWKADWTPYRLSLPRF"
     gene            1744..3207
                     /locus_tag="VLI90_08120"
     CDS             1744..3207
                     /locus_tag="VLI90_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HSV14211.1"
                     /translation="MPEAQHSPLWAWVTLLLSGLIVLGYAVIAFNWFFQSKLSKHEQA
                     RSALARLRNISFACALCGFVFFTTDTAWFLWRMYDGILLLLVAYTWSFLLRMRGLSLV
                     DERLAQMDELEQSAAKYREIAELLPHMVWTADADGRVDFSNQRWREYAGNNRSWLESV
                     HPDEQQQAIARWRTASTARVPISLELRLGGAGGYRMFLVKATTISRGNAVRWLGACAD
                     IEDQRLLAAQREAQAKQKSFWLNALSHDLRAPLHNVALNAHLLKLSAKDPGDVESVEM
                     IIENAAAAGDLVAKLLDFAKVGAQDNNVMEQVDVAALLHQVARRFQPVVEHRGLRLRV
                     LATEGASVLSDRQKLQRIVSNLVDNAIKYTRHGEVMLELSSRDDREENVVIRVTDTGI
                     GIPPAHAPHLFDEFYQVDNYERDRSKGFGMGLAICKDLARHLGGDVRLAHSSTDGSCF
                     EVVLKRNASGVGADRGGRPVGAPGDQPAAAVAGLCRV"
     gene            3137..3463
                     /locus_tag="VLI90_08125"
     CDS             3137..3463
                     /locus_tag="VLI90_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV14212.1"
                     /translation="MEDDRSARRAISRLLRSQGFAVCEAGTLADAMHGLHPEPPDWIL
                     LDLMLPDGCGTQLLRKMRADRIHSKTCVITGCASELLNEARRAGAEHVFTKPLNVDGL
                     MKVLMA"
     gene            3947..4258
                     /locus_tag="VLI90_08130"
     CDS             3947..4258
                     /locus_tag="VLI90_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV14213.1"
                     /translation="MGLVFSGVLTVMVGNAAGGVANTDRALTNFSSPMPMLSSAQTIV
                     SDPFASMLAPPAPAMFRAGLKSSHPEQDAVAAPEPTSLSLLAVGLGMIAVARRKRRWF
                     R"
     gene            4296..5267
                     /locus_tag="VLI90_08135"
     CDS             4296..5267
                     /locus_tag="VLI90_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSV14214.1"
                     /translation="MPAHAPASLPTVLVTEGSDATPLRWLRENANVIEIAPTDPAFDR
                     HLADAQGMVVRTYTKVNAALLDRAPKLKVVGRGGVGIENIDVGECRRRGIEVVYTPDA
                     NTLAVGDFVFGFLLQLLRPWAFFRERVYEPKEFKRIRDTVRGRQLNELTIGILGMGRV
                     GRRVGHIGANGFGMRVIYNDLVDVAGQLDFPAQSVDKPTLYREADILSIHVDMRAGNE
                     NLVGAAQIAQMKPTSVLINTSRGEVLDATALAAAIRERRLTGAAIDVFHPEPPPADFP
                     LLGLDNVLLTPHLAARTYTAIENMSWVVRDVIAVLSGEKPKFPAPAR"
     gene            5368..5859
                     /locus_tag="VLI90_08140"
     CDS             5368..5859
                     /locus_tag="VLI90_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14215.1"
                     /translation="MSPRRLWIVYALLAVLVGGHLYDALRFQEHWPFSNYPMFAGIAQ
                     PELTTYQLIGLSGDREVPIDNRAIPALPPYKFQGTLRRYDTTSKADPQKLRAMLSDYL
                     DAYETRRVRHLHDGPPLDGIRLYLVRIPIQLPPVPVDLKHGDHGDHPRMVMEVRRPDR
                     GAT"
     gene            5856..>6199
                     /locus_tag="VLI90_08145"
     CDS             5856..>6199
                     /locus_tag="VLI90_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14216.1"
                     /translation="MIRLSLRDRWNRFWFEPTEPQTLGFARLILMLGAFLYYHARNYE
                     SWGYVRPSFWQSTWLFLHLHWHPLSPMAMGWLGLAWKASLLLAGIGLFTRFSCAIAFA
                     LGLYLIGLPNNIG"
BASE COUNT         1158 a         1937 c         1954 g         1150 t
ORIGIN      
        1 atcgccgcac ccccatttcg ttaccccctc cttccatccc accagttgag gtgcgcacat
       61 gactgtcaca gaacggctcg agcgtcgtcg tttgttcagc gtcaccgtaa ccgaagactc
      121 gccgggattc ttcgaggtca acggtgacgg cggcgacaac gccatcaacg tcagcatttc
      181 gcagaacaat ggtacgttca cgctggacaa cacgacctac agcgaagttt cctacctcta
      241 cgtcaacggc aacggcggga acgacacgat cagcgtgacc agcagcgacg gcccggggaa
      301 catcggggcg agcatcgtca cgctggatgg gaatgacaac atcaccctcg gcgtcagcgg
      361 cggcgtgtgg gccggtggtg gcgatgacac ggtgaacgtc agcgattcgt actggggcga
      421 catcaacggt gaggctggca acgacaagat ttacatcagc ggtgacacga tcgacgcgca
      481 gatcagcggt ggatcaggta acgatttgat cgacgcgtcg aacaacaacg cgcctgtcac
      541 gatccacggc gatgcgggca acgactcgat ctacggctcg gccttcgacg acgaacttta
      601 cgccgacagc gggaacgatt cggtctgcgg caacgcgggc gacgacatcg tttacgcccg
      661 cctgagcaac ggcgacacga tcgatggcgg cgacggcacg gacgtgctgt acgccagcgg
      721 cagcgaagtt agcgtcaact atgtcgagta cgtcatttag ttcggccatg caagcgcgca
      781 gttggggatc acgcgcacgc gagcgccgtt accgaggttc aaggatgaac aagcggacca
      841 ttcgatcggg cggatttacg cttaccgagt tgctggtcgt cgtcggattg atcgcgctgc
      901 tgctggcgct gctgatgccg gtcgtcggca aggcccgggc ggcggcgaat tcgacggggt
      961 gcctttcgaa cctccggcag atgggcatcg cctggacgat gtacgcggcg gagaacaagg
     1021 gccggctgct ggagaatctc aagcccacgc cgttgaatcc tgacatcgcc tggcgcggct
     1081 actggacggg cgtgctcgat gggtaccagg tgcgcggcga tgcgctgctt tgtccggcgg
     1141 ccaatcgaac gatccccttc aaccagatca gcggatacgg caacgtgaac ttcgcgtgga
     1201 ccggcaagta tggtcccaat gccacctcgg ttcattataa cgccacgatc tatcgcagca
     1261 gcagctacgg gttcaacgaa tacctcgccg ccaacggcgg cttcgacacc gacagcctgg
     1321 cgacgaacat caccaagcta cgcccgctgg cggaggttcc cgtcttcgcc gattcggtgt
     1381 gggttgattt cgcaccgagg aatttcagcg ccggcttcgc gccggttcaa ccgccgccga
     1441 atttgcgcgg tgagggcttt actctaacgt cgccggatca ttggcggttt ttgatcgccc
     1501 gccacgggcg cgcggtgaac gtctgcttcg ccgatggcag cgccagtcgc gtgccgctcg
     1561 aggaaacata tctgctgcgg tggaaggccg attggacgcc ctatcggctt tcactaccca
     1621 gattctgaat cgacggcgtg cgtgcgtgcg tgcgcgcgtg ggggcatcac ggcatcggga
     1681 cggcacatga gcgtttgttc aatcgcggct ttggttgtgc caagcttcgc aggcggccga
     1741 tttgtgcccg aggctcagca tagtccgctg tgggcgtggg tcaccctgct gctcagcggg
     1801 ttgatcgtcc tcggttacgc cgtcatcgcg ttcaactggt tcttccaatc gaagttgtcc
     1861 aagcacgaac aggcgcgctc ggcattggcg cgcctgcgca acatctcgtt cgcctgcgcg
     1921 ctttgtggtt tcgtcttctt cacaaccgac accgcgtggt tcctctggcg gatgtacgac
     1981 ggcatcctgc tgctgctggt ggcctacacc tggtcgttcc tgcttcgcat gcgcgggctc
     2041 agcctggtgg acgaacggct ggcgcagatg gacgagctgg agcagtcggc ggcgaagtat
     2101 cgcgagatcg ccgagctgct gccgcacatg gtatggaccg ccgacgccga cggccgcgtc
     2161 gatttttcga accagcgctg gcgcgaatat gccggcaaca accgctcttg gctcgaatcg
     2221 gttcatccgg acgaacaaca acaggcgatc gcccgctggc gcacggcaag cacggcacgc
     2281 gtgccgatca gccttgaact ccgcctcggc ggggccggtg gatatcgcat gttcctcgtg
     2341 aaggcgacca cgatctcccg cggcaatgcg gtgcgctggc tcggggcgtg cgcggacatc
     2401 gaagaccagc gactgctcgc ggcgcagcgc gaagcgcagg cgaagcagaa gagcttttgg
     2461 ctcaacgcgc tgagccatga cctgcgcgcc ccgctgcaca acgtcgcgct gaacgcgcat
     2521 ctgctgaagc tgtcggccaa agacccgggc gacgtcgaga gcgttgagat gatcatcgaa
     2581 aacgccgccg ccgcgggaga cttggtggcc aagctgctgg acttcgccaa ggtcggcgcg
     2641 caggacaaca acgtgatgga gcaagtggac gtggccgcgc tgctgcacca ggtggcgcgc
     2701 cggtttcagc cggtcgtcga gcatcgcggg ctgcgcttgc gcgtcctcgc cactgaaggc
     2761 gccagcgtgc tgagcgatcg ccagaagctg cagcggatcg tcagcaacct cgtggacaac
     2821 gcaattaagt acacgcgcca tggcgaggtc atgctcgagc tttcatcgcg cgacgatcgg
     2881 gaagaaaacg tggtgatccg cgtgaccgac accggcatcg gcattccacc ggcacacgcg
     2941 ccgcatttgt tcgatgagtt ctatcaggtg gacaattatg agcgagatcg cagcaaagga
     3001 ttcggcatgg ggctcgccat ctgcaaagac ttggcccgcc acctcggggg cgatgtgcga
     3061 cttgcccaca gctcaaccga cggcagctgc ttcgaagtcg tgctcaagcg aaacgcatcg
     3121 ggcgtcggtg ctgatcgtgg aggacgaccg gtcggcgcgc cgggcgatca gccggctgct
     3181 gcggtcgcag ggctttgccg tgtgtgaagc cggcacgctg gctgacgcga tgcacggcct
     3241 gcatccggag ccgccggact ggatcctgct tgacctgatg ctgcccgatg gctgcggcac
     3301 acagttgctg cgcaagatgc gcgccgatcg catccacagc aagacgtgcg tgatcaccgg
     3361 gtgcgcgtcg gagctgctga acgaagcgcg ccgtgcgggc gcggaacatg tcttcacgaa
     3421 accgttgaac gtcgatggac tgatgaaagt gctgatggcg tagcgcggaa aaacaggaga
     3481 gccgggccgt cctcgccccg gacaggcccg gggacgggcc ggctctcccc acccccgtgc
     3541 ccgcatacca cctctggttc aacataagcg gagaaccccg taaaaccagg gctaagcgag
     3601 catctcgcaa gcaatccccc agaacctgct gccattttgt gcagcccgaa tgtcaaaccg
     3661 ggcgacagga ttgtcgcatc cgacaggtgc ttgtcgtgaa gatacagaag ctagcgcgaa
     3721 tggggaaaaa taccgcaagc accgggggta gggaaaatag ccgagcctgc tttcttaaac
     3781 cccgaatgcg ccatgtgatg atgcgcccaa caaaatgctg cgccggccca aactgcgact
     3841 tcggtacggc ctttgataga gcggaggcgg gactggctac gacacgttcg tgggttctct
     3901 atcagttggg caggacaaga ctgtgtatag atcggcaatt ccatggatgg gccttgtgtt
     3961 ttccggtgtg ctgacggtga tggttggcaa tgccgcggga ggcgtggcga acaccgaccg
     4021 cgctctcacg aatttctcat ctcccatgcc gatgctttcg tccgcccaaa ccatcgtcag
     4081 cgatccattt gcctccatgc tggcgccacc ggcaccggcg atgttccgcg cggggctgaa
     4141 gtcatcgcac ccagagcaag atgcggtggc ggcgccggaa ccgacatcgc tttcactgtt
     4201 agccgtcggc ttggggatga tcgccgtggc ccgccgcaag cggcgatggt tccgctaagc
     4261 ttcccgccac gcccgccgcc gctataactg gcgaaatgcc tgcccacgct cccgcttcat
     4321 tgccgactgt tctggttacc gaaggctccg acgccacgcc gctgcgctgg ctgcgcgaga
     4381 acgcgaacgt catcgagatt gcgccgaccg atccggcgtt cgatcgtcac ctcgccgatg
     4441 cccagggcat ggtcgtccgc acctacacca aagtgaacgc ggcgttgctc gatcgcgcgc
     4501 cgaagttgaa ggtggttggc cgcggcggtg tggggatcga gaacatcgac gtcggcgaat
     4561 gccgccgccg cggcattgaa gtcgtttaca cgcccgacgc caacacgctg gccgtcggcg
     4621 atttcgtgtt tggctttctg ctgcagctgc ttcgaccctg ggcgttcttc cgcgagcgcg
     4681 tgtatgagcc caaggaattc aagcgcatcc gcgacaccgt gcgcggccgg cagttgaacg
     4741 agttgacgat aggcatcctc ggcatgggcc gggtcggccg gcgtgtcggt cacatcggcg
     4801 ccaacggctt tggcatgcgc gtcatctaca acgatcttgt ggacgtcgcc ggccaactag
     4861 attttcccgc gcagtcggtg gacaagccga cgctgtatcg cgaggccgac atcctgtcga
     4921 tccacgtcga catgcgcgcg ggcaacgaaa acctggtggg cgcagcgcaa atcgcgcaga
     4981 tgaaaccaac gtcggtgttg atcaacacca gccgcggtga agtgttggac gcgacggcgc
     5041 tcgccgcagc gatccgggag cgtcgcttga ccggcgccgc gatcgatgtc ttccaccccg
     5101 agcctccgcc ggcggatttc ccgttgctgg ggttggacaa cgtgctgctc acgccgcacc
     5161 tggcggcgcg gacatacacg gcgatcgaga acatgagctg ggtcgtacgc gatgtgatcg
     5221 cggtactaag cggcgagaaa ccaaagttcc cggcgccggc gcgttgaatt tcgtaacacg
     5281 ggcgtctcgc ccgtgcctga acacttaggc acgggtgaga cgcccgtgct acagtaaatc
     5341 gcttattgga ccgaagaaga gtaagtgatg tccccccgcc gcctgtggat cgtttacgcg
     5401 ttgctcgccg ttctcgtcgg cgggcatctc tacgatgcgt tgcgcttcca ggaacactgg
     5461 ccgttctcga attacccgat gttcgccggc attgcgcagc cggagttgac gacgtaccaa
     5521 ctgatcggcc tgagcggtga ccgtgaagtg ccgatcgaca accgcgcgat ccccgcactt
     5581 ccgccttaca aattccaagg cacgcttcgg cgatacgaca cgacttccaa agcggatccg
     5641 cagaagcttc gcgcgatgct gtcggattat ctcgacgcct atgaaacgcg ccgcgtacga
     5701 cacctgcacg atggcccgcc gctcgacggc atccgtttgt acctggtgcg catcccaatc
     5761 caactgccgc cggtgcccgt cgatctgaag cacggcgacc atggcgacca tcctcgaatg
     5821 gtgatggaag tccgccggcc ggatcggggg gcgacgtgat tcgactctcg cttcgcgatc
     5881 gctggaaccg cttctggttc gaaccgaccg aaccgcaaac gctcggtttt gcgcggctca
     5941 tcctgatgct cggcgcgttc ctgtactacc acgcccgcaa ttacgaatcg tggggctacg
     6001 tccgcccgtc gttctggcaa agcacctggc tttttctcca cctgcactgg cacccgctgt
     6061 cgccgatggc gatgggctgg ctgggcttgg cgtggaaagc atcgctgctg ctcgccggca
     6121 ttggtttgtt cacgcgcttc agctgcgcga tcgccttcgc gctgggcctc tacctgatcg
     6181 gcctgcccaa caacatcgg
//
LOCUS       DATIMH010000471         3928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4438986, whole genome shotgun sequence.
ACCESSION   DATIMH010000471 DATIMH010000000
VERSION     DATIMH010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4438986"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1174)
                     /gene="phnD"
                     /locus_tag="VLI90_08150"
     CDS             complement(50..1174)
                     /gene="phnD"
                     /locus_tag="VLI90_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HSV14217.1"
                     /translation="MEPSDAGAAPQTPFSSDDAPPPRQREPHAFTTTHPFLLLIIIVL
                     GTAAVASALWAFYYRVPPANNKQVNLNAAGFNPPVANKLDPRFTDADGDLLADTPTDP
                     AQLLDPPTITFCYVPVEEPEKYRELWKGFCDHLSQVTGKPVEYAMFTTTKEQLRALHD
                     GKLQVAGLNTGAVPAAVNLTGFIPICAIPTGESTALQHTEIIVRADSPIQKPLDLKNH
                     ELTLTEPTSNSGYKAPLVLLRSNFGLEPLNDVLLRYSGSHEASIEGLASGKYEAAAVA
                     SDMLAHAVTQGDIKPNAYRSIYVSESFPTASLGYLYNLKPELAQKVKDAMLSYDWKGT
                     PLAEHFATDKRTTFVPVNYKNDWSLIRRIDDEMGIQQKIE"
     gene            1469..2788
                     /locus_tag="VLI90_08155"
     CDS             1469..2788
                     /locus_tag="VLI90_08155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV14218.1"
                     /translation="MTLSPPSQPLEYQAKAAALPGARAALVLLLLINLFNYIDRPVLA
                     AVEHDIAKDFFKDPNAPATMAKMGSLASAFFFSYMLTAPLFGFLADRMSRWLLVGTGV
                     LLWSLATGATGLAGSFGILFASRLFVGIGEAGYGPSAPTIISDLYPVERRGGVLAWFY
                     VAIPVGSALGYVIGGAVGGAMGWRWAFYVVTIPGILLGLWSLRMKDPKRGGADAPAGT
                     ADAQAKPTMRDYLSLLHIPSYVLNTAAMAAMTFAIGGIGYWMPRYLIDVRGLATAKAN
                     TMFGAIVVVAGLGATLLGGWAGDKLRPRFPGSYFLVSAVGILLACPFVILMLHVPFPF
                     TWLALFGAVFFLFFNTGPSNTALANVTHPSVRASAFALNIFLIHLLGDVPSPTVLGYV
                     AGKFGWDAAFGVVAALMAVAGVLWFIGTQYLAADTAAAPHRRAPAPA"
     gene            2826..3224
                     /locus_tag="VLI90_08160"
     CDS             2826..3224
                     /locus_tag="VLI90_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14219.1"
                     /translation="MIAASPPGTCGRDWRSSGPYAITPLASLSPTLSFVWEGSGITEP
                     EINAPRAGRGDSAFLANAVLLRRLIMSGRDNQRSDADIERIQSEARTLRLRSDELTKK
                     LIALTKKIADLEEEQRVRRAHLAERNRHGA"
     gene            complement(3322..>3928)
                     /locus_tag="VLI90_08165"
     CDS             complement(3322..>3928)
                     /locus_tag="VLI90_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14220.1"
                     /translation="LKNFTLPPMPAVSGPVAVGLHEIINWGDGSAPSAGRLVPSDTIA
                     RQFNVVGSHTYAKAGTFKITVNVVWGPPPGSALMMPTTILATIHSTAVVAKDDDGGVS
                     LVEVAGRSFKAKVGSFDFRSVDLILSATIDWGDGTHSAGTIAGNFATGEYNVIGSHTY
                     AKTGQYAVQVKVLSRVFGAPSIVPSGTVAQWTSTIKVLGSA"
BASE COUNT          633 a         1223 c         1285 g          787 t
ORIGIN      
        1 cgtcttttcg atctgaagaa acagatgcta agctgcaagc ggcgggaact cattcgatct
       61 tctgctgaat ccccatctcg tcgtcgatcc gccggatcag cgaccaatcg ttcttgtaat
      121 tcaccggcac gaaggtcgtc cgcttgtcgg tcgcgaaatg ctcggccagc ggcgtgcctt
      181 tccagtcgta gctgagcatc gcgtccttca ccttctgggc gagctcgggc ttgaggttgt
      241 acaggtaacc caggctggcg gtggggaaac tttcgctgac gtagatcgag cggtaggcat
      301 tgggcttgat gtccccctgc gtcaccgcgt gcgcgagcat gtccgacgcc accgcggcgg
      361 cttcgtactt gccgctggcc aggccttcga tcgacgcttc gtgcgagccg gagtaacgca
      421 gcagcacgtc gttcaacggc tcgaggccga agttcgaacg cagcagcacc agcggcgctt
      481 tgtagccgct gttggacgtc ggttcggtca gcgtcagctc gtggttcttc aaatcgagcg
      541 gcttctgaat cgggctgtcg gcgcggacga tgatctcggt gtgctgaagc gctgtgcttt
      601 cacccgtggg gatcgcgcag atcgggatga agccggtcag gttcaccgcg gcgggaacgg
      661 cgccggtgtt caggccggcc acctgcagct tgccatcgtg cagcgcgcgc agctgctcct
      721 tggtcgtcgt gaacatcgca tactcaacgg gcttgccggt cacctgcgac aggtgatcgc
      781 agaaaccttt ccacagctcg cgatatttct caggctcttc aaccggcaca tagcagaacg
      841 tgatcgtcgg tggatcgagc aactgcgccg gatcggtcgg tgtgtccgcc agcagatccc
      901 catcggcatc cgtgaagcgc gggtcgagct tgttggccac cggtgggttg aatccggcgg
      961 cgttcaggtt cacctgcttg ttgttcgccg gcggcacccg gtaataaaat gcccacaacg
     1021 ccgacgccac cgcggcggtg ccgaggacga tgatgatcag cagcaaaaac ggatgcgtcg
     1081 tcgtgaaggc gtgcggttcc cgttgccggg gcgggggcgc gtcgtcagac gaaaacgggg
     1141 tctgcggtgc tgctccggcg tctgaaggtt ccatgacgtg tccttaaatg tcgatgcggc
     1201 ggcgcgaaac gtaggctctc ccacgacggg cgaacattct aacccagcgt gtgcgggagg
     1261 cccaaacttc ttttgctggg aagcgacaag actttgccgg acggagcggc gactgatggc
     1321 tggacgacga actttttagc caccgaagaa tgccagcggg acgttccaga ggcaaatgat
     1381 tcggtgctcg ccaagacccg ctggcggtcg cctgcggctg ttaacgcgtt gcaataacgc
     1441 ctgctcacgg ccatcatgcc gcgcacgcat gacgctttcg ccgccgtctc aaccgctgga
     1501 gtaccaagcc aaagccgcgg cgctgcccgg ggcacgggcg gcgctggtcc tgctgctgct
     1561 catcaatcta ttcaactaca tcgatcgccc ggtgctggcg gcggtcgaac acgacatcgc
     1621 caaggatttt tttaaggacc ccaacgcgcc ggcgacgatg gcgaagatgg gatcgttggc
     1681 cagcgcgttc ttcttcagct acatgctgac cgcgcccctg ttcggatttt tagccgatcg
     1741 aatgtcgcgt tggctgctgg tcggtaccgg ggtgctgctt tggagtctgg caaccggcgc
     1801 aacgggtctc gctggttcgt tcggcatcct gttcgccagc cggttgttcg tcggcatcgg
     1861 tgaggccggc tacgggccgt cggcgccgac gatcatctcg gacctgtacc ccgtcgagcg
     1921 ccggggtggc gtgctcgcgt ggttctacgt cgcgatacct gtcggcagcg cgctcggtta
     1981 cgtgatcggc ggggccgtcg gtggggcaat gggctggcga tgggcgttct acgtcgtcac
     2041 gatccccggc atcctgctcg gcctgtggag cctgcgcatg aaggatccca agcgcggcgg
     2101 cgccgatgcg ccggccggca ctgccgatgc gcaggccaaa ccaacgatgc gcgattacct
     2161 gtcgctgctc cacatcccat cgtacgtgct caacactgcg gcgatggccg cgatgacctt
     2221 tgccatcggc gggatcggtt actggatgcc ccggtacctg atcgacgtgc gcggcctggc
     2281 gaccgcaaag gcgaacacga tgttcggcgc gatcgtcgtc gtcgccggtc taggcgcgac
     2341 gttgctcggc ggttgggctg gcgacaagct gcggccgcgg ttccccggtt cgtatttcct
     2401 ggtgtcggcc gtcggcatcc tgctggcgtg cccgttcgtc atcctgatgc tgcacgtgcc
     2461 gtttccgttc acctggctcg cgctgttcgg cgcagtcttc ttcctgttct tcaacaccgg
     2521 cccgtcgaac accgcgctgg cgaacgtgac gcacccatcg gtgcgcgcgt cggcgttcgc
     2581 gctcaacatc ttcctgatcc acctgctcgg cgacgtgccg tcaccgacgg tgctgggtta
     2641 cgtcgcggga aaattcggct gggacgccgc gttcggcgtc gtcgccgcgc tgatggcggt
     2701 cgctggcgtg ctatggttca tcggcacgca gtatctcgcc gccgacaccg ccgccgcccc
     2761 gcatcgacgc gcgccggcgc cggcgtgagg caacccgtct gttcattgtc agattgtttg
     2821 ggccgatgat tgccgcatcg ccgccaggca cgtgcggcag agattggcgc tctagcggtc
     2881 cgtatgctat tacccctctc gcgtcgctct cgcctacttt gtcttttgtt tgggagggaa
     2941 gcggcatcac agagcctgaa ataaatgcgc cgcgcgccgg tcgcggcgac tcggcatttc
     3001 ttgccaatgc ggtactattg cggcggctca tcatgtctgg ccgtgacaat cagcgcagcg
     3061 atgcggatat cgagcggatc caatccgaag ctcgaacgct ccgattgcgg tccgatgagc
     3121 tgacgaaaaa gctgatcgcg ctgaccaaga aaatcgccga cctggaagag gagcagcgcg
     3181 tcagacgcgc tcatctggcg gagcgcaacc ggcacggcgc gtgattggct tgctgcgccc
     3241 ggagctttgg aaccagctgg gttagggggc gacaccgaag aggaacacca gacgcgcacc
     3301 ccttcggctc ttcgcgatcg atcacgcgct gcccagcacc ttgatcgtgc tggtccattg
     3361 cgcgacggtg ccgctgggca cgatgcttgg ggcgccgaac actcgcgaga gaacctttac
     3421 ctgcacggcg tattggccgg ttttcgcgta cgtgtgtgag ccgatgacgt tgtattcgcc
     3481 ggtggcgaag ttgccggcga tcgtgccggc cgagtgcgtg ccgtcgcccc agtcgatggt
     3541 cgccgataag atcaggtcca cgctgcggaa gtcgaacgag ccaacctttg ccttgaacga
     3601 tcgaccggcg acctcgacga ggctgacgcc gccgtcgtcg tctttggcga cgacggccgt
     3661 gctgtgaatg gtcgccagga tggtggtcgg catcatgagc gccgagcccg gcgggggtcc
     3721 ccagacgacg ttgacggtga tcttaaaggt gccggccttg gcgtaggtgt gcgaaccgac
     3781 gacgttgaac tgccgcgcga tcgtgtcgct cggcaccaac cgcccggcgg acggcgcgga
     3841 gccgtcgccc cagttgatga tctcgtgcaa ccccacggcc accggtccac tgacggccgg
     3901 catcggtggc agggtgaagt tcttcagg
//
LOCUS       DATIMH010000472         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4732903, whole genome shotgun sequence.
ACCESSION   DATIMH010000472 DATIMH010000000
VERSION     DATIMH010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4732903"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VLI90_08170"
     CDS             complement(<1..233)
                     /locus_tag="VLI90_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14221.1"
                     /translation="MKSLAVLLAEDRRCAILRTLVEVDGAANESILRQSLALLGHQRE
                     LTKDNVRADLNFLSDCGLVALEWFGDKVCVAKIT"
     gene            complement(230..643)
                     /locus_tag="VLI90_08175"
     CDS             complement(230..643)
                     /locus_tag="VLI90_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14222.1"
                     /translation="MSAVVQTWWPVLTGVLTVLLAVATLWVNSKVDKAIKEERVDEKI
                     ADAVQKVMDKVEKGDGANSGLFTMINERVAKVEGACAGFAPASDFNTALLKIVEMGGD
                     MKAIRAELVALHNDGKALKHSVDRLYDVQLESNRS"
     gene            complement(886..1242)
                     /locus_tag="VLI90_08180"
     CDS             complement(886..1242)
                     /locus_tag="VLI90_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14223.1"
                     /translation="MDNQTTGQSTDNGAQQDKGGFNLGSFVQQVSAFVSATAPIVDAG
                     VGIALPELAPAIDAGTKILQGVLAEEPAAKALYDQIKSGETPTADQIAAIESSYEADY
                     QQLKADIAAAKAAQAT"
     gene            complement(1257..1610)
                     /locus_tag="VLI90_08185"
     CDS             complement(1257..1610)
                     /locus_tag="VLI90_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14224.1"
                     /translation="MAAALMLPLYACGTTPVDIVQNLTTSTPVQVTTYADATAAADLV
                     TRTADLAVATGKLPSSVLDAIGSYSDKVHSEWLKLKAAKDAGQSLAFQAFQDALSAFE
                     SYRTAQGIPEAPAKS"
     gene            complement(1671..2204)
                     /locus_tag="VLI90_08190"
     CDS             complement(1671..2204)
                     /locus_tag="VLI90_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14225.1"
                     /translation="MLRRILSNPLGELLTLSLLILLLIVCGSPHAFAQVVSAPATAPT
                     IVQVQPFLHDVLMLVGIVVIAVLPFIVQPVAKAAAQHFHLSNTTAVSAVLTKLAYDAA
                     NYGIAYCDSQVTKVRGINVGDPKIASAANWLLTHASDEIEHLNLSNQEVIDTVKAILP
                     QALAATPLRAGTATAAT"
     gene            complement(2221..2748)
                     /locus_tag="VLI90_08195"
     CDS             complement(2221..2748)
                     /locus_tag="VLI90_08195"
                     /inference="COORDINATES: protein motif:HMM:NF020931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HSV14226.1"
                     /translation="MEFDRVVMIEGGYVNSPTDSGGATKFGITEAVALAHGYVGAMKD
                     LPLETAKDIYRVEYWNQLNLDPVSQLSQPLAHELFDTGVNCGIGTASRFLKRALNALN
                     RGGKDWADVPLDSPIGPQTLHALQRLHVVRGEEGLTVLLRMLNAQQSVRYLEIAERRP
                     RDEVNEFGWQDNRVS"
     gene            complement(2874..3287)
                     /locus_tag="VLI90_08200"
     CDS             complement(2874..3287)
                     /locus_tag="VLI90_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14227.1"
                     /translation="MNLPAQSLPHILRRIRDVGGMDAVLKLVKVFGGKRIFIPRYDVS
                     AGHPLALAGTPVAKMLVDEFGGNHVDFPRGRAHIRLMIARDVHAEGGSTNDIAQAADI
                     SHQRAKQLRRMVRNKKQPASAGRPRKRNAHQRELI"
     gene            complement(3284..3976)
                     /locus_tag="VLI90_08205"
     CDS             complement(3284..3976)
                     /locus_tag="VLI90_08205"
                     /inference="COORDINATES: protein motif:HMM:NF018011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HSV14228.1"
                     /translation="MTLAAMKACAPARAARLRVLHQTTRRLGIDDETYRARLVREFGV
                     RSASLLTDAQLDRAIDMFHVKQKVTLQPHAKLVRALWIALSNLGAVDRTDAALDHFVE
                     RQTGKQKLQFLTASESNSVTEALKAMCAREGFEVKGDPRDDRIALIRAQWRKMSVTPR
                     STVHGDMMLSSGRDLSLTDYVSRKYRLAHGAGLSRLSIDQLDDCARAFGTKLRNALAL
                     QAAAAAKRASAA"
     gene            complement(3973..>4216)
                     /locus_tag="VLI90_08210"
     CDS             complement(3973..>4216)
                     /locus_tag="VLI90_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14229.1"
                     /translation="DFPRRSPPFGHLLQPWCRDCKNAYGRAKKAELSPVARAKRTDAM
                     VRNVIFNVEQNPAAHTVEEVIRNLRDALGLKEGERP"
BASE COUNT          732 a         1321 c         1325 g          838 t
ORIGIN      
        1 gtgatcttcg ccacgcacac cttgtcgcca aaccactcca aggcgacgag cccgcaatcg
       61 ctcagaaaat tgaggtccgc gcgaacgttg tccttggtga gctcgcgctg atgaccgagc
      121 aacgccaggc tctggcgcaa aatgctttcg ttcgcagcac cgtcaacttc aacgagagtc
      181 cggaggatgg cgcagcgacg gtcttctgcc agaagcacgg caaggctttt catgaccgat
      241 tgctttcgag ctgaacgtcg tacagccgat cgaccgagtg cttcagcgct ttcccgtcat
      301 tgtgcagggc caccaattct gcccggatcg ctttcatgtc gccgcccatt tcgacgatct
      361 tgaggagcgc cgtgttgaag tcggatgcgg gggcgaaccc ggcacacgcg ccctccacct
      421 tggcgacgcg ctcattgatc atcgtgaaga gtccgctgtt ggcgccgtcc cccttctcga
      481 ctttgtccat caccttctga acggcgtccg cgatcttctc gtcgacgcgc tcttccttga
      541 tcgccttgtc gaccttcgaa ttgacccaga gggttgcaac ggcaagcaac accgtgagaa
      601 ccccggtgag caccggccac catgtctgca ccaccgcact catcgcgtgc tcacctgttc
      661 actctttcga aaaggcgctg acacccgacg cagcgcgtgg ccgatggaat ggcttttcgg
      721 cgctcttcag gaatgggatc accgcaacgt tcgcaatcct gaccgccgca taccgcgcgc
      781 gccgcggcca cttgtctgcg gtgacgtgcc agcgcagcgg cgcgataatc ttcgtcgcgc
      841 tgctgcgcct ggtcgaactc gtctggcacg cccccacttc gccccttacg tcgcctgtgc
      901 ggccttggcg gcggcgatgt cggccttcag ctgctgatag tcggcttcgt agctcgactc
      961 gatcgccgcg atctgatcgg cagttggcgt ctcgccagac ttgatctgat cgtacaaagc
     1021 ctttgccgca ggctcttccg cgagcacgcc ctgcaggatt ttcgtaccgg catcgatagc
     1081 cggcgcgagt tccggtagag caatgccgac gcctgcgtcg acgattggtg cggtggcact
     1141 tacaaaggcc gaaacctgct gcacgaacga gcccaggttg aagccgccct tatcctgctg
     1201 ggcaccgttg tcggtgcttt ggccggtggt ctggttgtcc attatccgtc cctccttcag
     1261 gacttcgcgg gcgcttcggg aatcccctgc gccgtgcgat agctttcgaa cgcgctcagc
     1321 gcgtcctgga acgcctggaa ggcgagcgac tggccggcat ctttcgcggc cttcagcttc
     1381 agccactcgg aatgaacctt gtcgctgtag ctgccgatcg catccagcac cgacgaaggc
     1441 agcttgcctg tggcgacagc gagatcagcc gtgcgcgtga ccaggtcggc cgctgccgtc
     1501 gcgtccgcgt aggtggtcac ctgcaccggc gtggacgtcg tgagattttg cacgatgtcc
     1561 accggggtgg tgccgcaggc ataaagcgga agcatcagcg cggcggccaa ggctccgatt
     1621 gcaagtagtt tcacaaacag tctcctctcg aatgctcccg cgcgattgcg tcaggtagcg
     1681 gcggtcgccg tgccggcgcg gagcggggtc gctgcgaggg cttgcggcag gatggctttg
     1741 acggtgtcga tcacctcctg attgctgaga ttcaggtgtt cgatctcgtc actggcgtgc
     1801 gtcagcaacc agttcgcggc ggaggcgatc tttggatcgc cgacattgat gccgcgcacc
     1861 ttcgtcacct gagaatcgca ataggcgatg ccgtagttcg cggcgtcata ggcgagcttt
     1921 gtcagcaccg cgctcacggc cgtcgtgttc gacaggtgga agtgctgtgc ggcggctttg
     1981 gcgaccggtt gcacgatgaa cgggaggacc gcgatcacaa cgatcccgac gagcatcagg
     2041 acgtcgtgaa ggaacggctg cacctgcacg atcgtgggcg ccgtcgccgg cgcggacacc
     2101 acctgggcga atgcatgcgg cgagccgcag acgatgagca acagaataag cagcgagagc
     2161 gtgagcaact cgccaagtgg attcgacagg atacgacgca acataaaagt ttctccctct
     2221 tcagctcaca cgattgtcct gccagccgaa ttcgttgact tcgtctctcg gccggcgttc
     2281 agcaatttcg agatagcgga cgctctgctg cgcgttcagc atccgaagca gaacggtgag
     2341 tccttcctcg ccgcgcacga cgtgaagccg ctgcaaagcg tgaagcgtct gtgggccgat
     2401 cgggctgtcg agcggcacgt ccgcccaatc cttgccgccg cggttcagcg cattcagagc
     2461 gcgctttagg aagcggctgg cggtgccgat gccgcaattg acgccggtgt cgaacagctc
     2521 atgcgccagc ggctgtgaca gctgagagac aggatcaagg ttcagctggt tccagtactc
     2581 gacccgatag atgtccttcg ctgtttccag cggcaggtct ttcatcgcgc cgacatagcc
     2641 gtgggcaagc gcgaccgctt cggttatgcc gaatttcgtc gcgccgccgg aatccgttgg
     2701 gctgttgacg tagccgccct cgatcataac gacgcgatcg aattccacat cgaaagcggt
     2761 cgggcgggga tttcccccgc ccgtcgcgct cgaagaagag acggaactgc ccatttgcag
     2821 cgacagtgcg ccgcgtgcgg acgtctattc aggggaaaat attttccctc gtctcagatg
     2881 agctcgcgct ggtgcgcatt gcgtttgcga gggcggccgg cagacgcagg ctgtttcttg
     2941 ttgcgcacca tgcggcgcag ctgcttggca cgctgatggc tgatatcggc ggcctgggcg
     3001 atgtcgttgg tactgccgcc ttcggcgtgc acgtcgcggg caatcatcag ccgaatatgc
     3061 gcacggccgc gggggaagtc gacatggttc ccaccgaact cgtcaaccag cattttcgcc
     3121 accggcgtgc cggccagcgc cagcggatga ccggcgctca cgtcgtagcg cggaatgaag
     3181 atgcgcttgc cgccgaagac cttcaccagc ttcagcacag catccatgcc gcccacgtcg
     3241 cggatgcggc gcaggatgtg cggcaggctc tgtgctggaa ggttcacgcc gccgacgccc
     3301 ttttggcagc tgctgcggcc tgcagcgcca gcgcgttgcg gagttttgtg ccaaaggcgc
     3361 gggcgcaatc atcgagctgg tcgatgctga ggcgtgaaag cccggcgccg tgcgcgagcc
     3421 ggtattttct gctcacgtag tcggtgagcg agagatcacg gccgctcgac aacatcatgt
     3481 caccgtgaac ggtcgagcgc ggcgtgacac tcatctttcg ccactgggcc cggatgaggg
     3541 caatgcgatc gtcgcggggg tcgcctttca cctcgaaacc ttcgcgcgcg cacatggcct
     3601 tcaacgcctc ggtgaccgaa ttggattcac ttgcggtgag gaactgaagc ttctgcttgc
     3661 cggtctgccg ctcgacgaaa tggtcgaggg cggcgtcggt gcggtccacg gcgccgagat
     3721 tggagagcgc aatccatagc gcgcgaacca gctttgcatg tggctgcaaa gtcacctttt
     3781 gcttcacgtg gaacatgtct atggcgcgat cgagctgggc gtcggtgaga aggctggcgc
     3841 tgcgcacgcc gaattcccgc acgagccggg cgcgataggt ttcgtcgtcg atcccgagac
     3901 gccgggtcgt ctggtgcagc acccgcagcc gggcagcgcg cgccggcgca caggctttca
     3961 tggcggccag cgtcatgggc gctcgccttc cttgagccca agggcgtcac gaagattgcg
     4021 gatcacttcc tcgacagtgt gggcggccgg attttgctcg acgttgaaga tgacgttgcg
     4081 gaccatcgcg tcggtccgct tggcacgggc aactggcgac agctccgctt tcttcgcgcg
     4141 gccatatgca ttcttgcagt cgcgacacca cggctgcagc aggtgtccaa aaggcggtga
     4201 gcgccgcggg aaatcc
//
LOCUS       DATIMH010000473         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1554181, whole genome shotgun sequence.
ACCESSION   DATIMH010000473 DATIMH010000000
VERSION     DATIMH010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554181"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VLI90_08215"
     CDS             complement(<1..1041)
                     /locus_tag="VLI90_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSV14230.1"
                     /translation="MFQLGPLVIRNVLRNRRRSILTLASAAVSLAVLGFLVALYQGFF
                     FAEQTSPSEALRLIARHKVSLTNPLPASYGPRIAVIDGVQAVSAWSWFQGVYIDNKPE
                     NFFARFAVDPAEIQKVHPEYTGPAEQWTAFQRNRTGCAVARTIAEKHNLKVGDRVNII
                     GDIYPVNPELTVELIFDHPKNTECMMFHRDYISELMKAQGHDADTVGTFSILARSTDD
                     VPRIARAVDTMFENSSYPTKTESEREFSLSFMAFMGNIKLYLAAICSAVTFTILLVSA
                     NSIAMSVRERTREMGILRTLGFTPGEIIGMVLGESILIALLGGLLGMAVTAGLTRAAA
                     AGMGPWGAMFKFR"
     gene            1224..>2663
                     /locus_tag="VLI90_08220"
     CDS             1224..>2663
                     /locus_tag="VLI90_08220"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HSV14231.1"
                     /translation="MFDTYEVNGLFDEMFSAAGTPRPHYVAAAARLKAAGAESFARRV
                     KMADVTFRNQGITFTVYADQRGVEKIFPFDLIPRIIPASEWDQIERGLEQRITALNLF
                     CHDIYHEQRILREKIVPPELIYGAKMFRREMFHVNVPRNIYIHICGTDLIRDRDGKYF
                     VLEDNGRTPSGVSYVLENRAVMKRVFPSLFSAYRVRAIEDYPHNLLQCLKYVAPQHAV
                     DPTIAILTPGIYNSAYFEHSFLARQMGVELVEGRDLLVENGFVYMRSTAGLRRVDVIY
                     RRVDDDFLDPLCFRPDSGLGVAGLMNAYRLGNVALANAVGTGVADDKAIYPFVPDMIK
                     FYLGQDAILPNVPTYICARDSDRKYVLENLDKLVVKSTNESGGYGMLMGHQAAADERE
                     KFAAKICQDPRNFIAQPVVELSQHPSLCDGQRFEGRRIDLRPYILAGEKVIVMPGGLT
                     RVALRKGSLVVNSSQGGGSKDTWVLEEEEP"
BASE COUNT          510 a          829 c          858 g          466 t
ORIGIN      
        1 gcggaatttg aacatcgcgc cccacggtcc catgcccgcg gcggccgcgc gcgtcagccc
       61 cgccgtgacc gccatgccca gcagcccgcc gagcagcgcg atcaggatcg attcgcccag
      121 caccatgccg atgatctcac ccggcgtaaa gcccagcgtg cgcaggatgc ccatctcgcg
      181 cgtgcgctcg cgcacgctca tcgcgatcga gttggccgag acgagcagga tcgtgaacgt
      241 gactgccgag caaatcgccg ccaggtaaag cttgatgttg cccatgaacg ccatgaacga
      301 caggctgaat tcccgctcgc tttcggtctt ggtggggtac gaggagttct cgaacatcgt
      361 gtccaccgcc cgcgcgatgc gcggcacatc gtcggtcgag cgcgccagga tcgaaaacgt
      421 gcccaccgtg tcggcatcgt gtccctgggc cttcatcaac tcgctgatgt agtcgcggtg
      481 gaacatcatg cattcggtgt tcttcgggtg atcgaagatc agctcgacgg tcagctccgg
      541 gttcaccggg tagatgtcgc cgatgatgtt cacgcgatcg ccgaccttga ggttgtgctt
      601 ttcggcaatc gtccgcgcga cggcgcaacc ggtgcgattg cgctgaaacg ccgtccactg
      661 ctcggccggg ccggtgtatt ccggatgcac tttttggatt tcggctggat cgacggcgaa
      721 gcgcgcgaag aagttctccg gcttgttgtc gatgtacacg ccctggaacc aggaccacgc
      781 ggagacggcc tgcacgccgt cgatcaccgc gatgcgcggg ccatagctag cgggcaaggg
      841 gttggtcagc gacaccttgt gccgcgcgat cagccgcaac gcctcggatg gcgaggtctg
      901 ctcggcaaag aagaatccct ggtacagcgc gacgagaaac cccagcaccg ccagcgacac
      961 cgcggcactg gccaacgtca aaatgctgcg ccggcggttg cgcagcacgt tacggatgac
     1021 gagcggaccc agttgaaaca tcgcagaacg ccctgagcaa aatgacgatt cctggcgatg
     1081 ctaagttggg cgagaagccg ggtcaaatcg ctcgcgttca atccgatcgc aacactcctt
     1141 catgtacacg cggggcttct tgtcttagaa tcccgcccgg cggagtgagg gcggaacgcg
     1201 aggggatcgg aaatccgctc ggcatgttcg acacgtacga agtcaatggt ctgttcgacg
     1261 agatgttctc ggcggcggga acgccgcgcc cgcactacgt tgccgccgcg gctcgcctga
     1321 aggccgccgg cgccgaatcg ttcgcccgcc gggtgaagat ggcggacgtc accttccgca
     1381 accagggcat caccttcacc gtctacgccg accagcgcgg cgtcgagaag atctttccgt
     1441 tcgacctgat cccccgcatc atcccggcca gcgaatggga ccagattgaa cgcgggctcg
     1501 agcagcgcat caccgcgctg aacctgttct gccacgacat ctatcatgag cagcgcatcc
     1561 tgcgcgagaa gatcgtcccg ccggagctga tctacggcgc caagatgttc cgccgcgaga
     1621 tgttccacgt gaacgtcccg aggaacattt acatccacat ctgcggcacc gatctgattc
     1681 gcgatcgtga tggcaagtat ttcgtgctgg aagacaacgg ccgcacgccg agtggcgtga
     1741 gctacgtcct ggaaaatcgc gcggtgatga agcgggtgtt cccgtcgctg ttcagcgcgt
     1801 atcgcgtgcg cgcgatcgag gattatccgc acaacctgct gcaatgtttg aagtacgtgg
     1861 ccccgcagca cgcggtggac ccgacgatcg cgatcctcac gccggggatc tacaacagcg
     1921 cgtatttcga gcacagcttc ctggcgcggc agatgggcgt ggagctggtc gaaggccgcg
     1981 acctgctggt ggagaacggg tttgtttaca tgcgatcgac cgcggggttg cggcgggtgg
     2041 acgtgattta ccgccgggtg gacgacgact tcctcgatcc gctttgcttc cggcccgatt
     2101 cgggtctggg cgtggccggg ttgatgaacg cgtatcgcct gggcaacgtc gcgctggcca
     2161 acgccgtggg caccggcgtg gcggacgaca aggcgatcta cccgttcgtg ccggacatga
     2221 tcaagtttta tctgggccag gacgcgatcc tgccgaacgt gccgacgtac atctgcgcgc
     2281 gcgacagcga ccgcaagtac gtcctggaaa acctcgacaa gctggtggtg aaaagcacga
     2341 acgagtcggg cggatacggc atgctgatgg gccaccaggc ggcggcggac gaacgcgaga
     2401 agttcgcggc gaagatctgc caagacccgc gcaacttcat cgcgcagccg gtggtcgagt
     2461 tgagccagca ccccagcctg tgcgacggcc agcgcttcga aggccggcgg atcgacctgc
     2521 ggccgtacat cctggccggc gagaaagtga tcgtcatgcc cggcggattg acgcgcgtcg
     2581 cgcttcgcaa gggctcactg gtcgtgaact ccagccaagg cggcgggagc aaggacacgt
     2641 gggttctgga agaagaagaa ccg
//
LOCUS       DATIMH010000474         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_110542, whole genome shotgun sequence.
ACCESSION   DATIMH010000474 DATIMH010000000
VERSION     DATIMH010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110542"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            561..1586
                     /locus_tag="VLI90_08225"
     CDS             561..1586
                     /locus_tag="VLI90_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSV14232.1"
                     /translation="MQRAEFVVTDPPFASCHAATLVESGGAICCAFFGGSREGKPDVC
                     IWFCRRDGDSWSEPIVVGDGAQADGQRFATWNPVLFQPSGGPLMLFYKVGPSPSKWWG
                     VVRTSDDAGRTWSDPRRLPAGILGPAKNKPVQLGSAILSPCSDEHPENGWRIYFERSD
                     DGGATWQATPPVEQPPEIPKATQPTILVHSAHKLQAICRTTIGRLCETWSNDGGRTWQ
                     PLRLIDLPNCNSGVDAVTLRDGRHLLVYNHSNLEKVRYPLNVAITRDGATWQAAAVLE
                     SEPPGQYSYPAVMQSLDGLVHIAYTFKRRNIKYVVMDPSAIEPQPIEMFVCPPPGSPT
                     HRSDEFQ"
     gene            complement(1739..>2102)
                     /locus_tag="VLI90_08230"
     CDS             complement(1739..>2102)
                     /locus_tag="VLI90_08230"
                     /inference="COORDINATES: protein motif:HMM:NF027673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HSV14233.1"
                     /translation="TADRATRKDAFYFYKANWSPEPFVYITSRRYTPHPPGKRAVKVY
                     SNCQTVELVMNGQSLGRVTAADHVFVWPDVMLSEGRCELKAIGQRDGRTLTDACTWTI
                     SNAAATTHPTTTSVYEAP"
BASE COUNT          398 a          640 c          662 g          402 t
ORIGIN      
        1 ggagcgacca ggcatccagc cgtggtgttc gttcgtccac atgagcatcg cgcccgtgat
       61 ctgtcgcatg ttgctcaggc aggcgacctg ctgcgcggac ttacgcgcct tctgcagcgc
      121 ggggagcaga atgccgatga gcagcgctat gatgccaatg acaacaagca actcgacgag
      181 tgtgaatgcc cggcgacgag cagaaggtgt gcgtcggcga taatacgtat gggagcaggt
      241 caactgcatc acggacctcc gggatgattt tttcaaggct acgcgtcacg gcgaaatgct
      301 tgactgcgcg acacgcggcg ttccgccgtt cgttcggacc ctgcgagagt cagagtctca
      361 ttcgacgttg aagtatatag caccttccta cacccccaac gttcccattt tgcgtaagag
      421 ttttgcaatc ttgcgcattg agggctcgcc ccgatgggtc tgtcacgcgc gcattgccgg
      481 cgcatcgcgc gtggcgcgcc cgccatgcag tatgatccgg gcgctcgaaa ggacccgtgt
      541 attcgatgaa atcttcactc atgcagcgtg ccgaatttgt tgtgacggac ccgccgtttg
      601 cttcgtgtca cgccgcgacg ttggtggaat cgggcggcgc gatctgctgc gcgttcttcg
      661 gcggcagccg cgaaggcaag ccggatgtgt gcatctggtt ttgccgtcgc gacggcgatt
      721 cgtggagtga gccaatcgtt gtcggcgacg gcgcgcaagc ggatggacaa cgcttcgcga
      781 cgtggaatcc ggtgttgttt caaccgagcg gtggtccgct gatgctgttc tataaagtcg
      841 ggccgtcgcc gtcgaagtgg tggggcgtcg tgcgaacctc ggacgatgcc gggcgaacgt
      901 ggagcgaccc gcggcggctg cccgcgggga ttctcgggcc ggcgaagaac aagccggtgc
      961 agttgggcag cgcgatcctt tcgccatgta gtgacgagca tcccgagaac ggctggcgga
     1021 tttatttcga gcgcagcgac gatggcggcg cgacctggca agcaacgccg cccgtcgagc
     1081 aaccgccgga aatccccaag gcgacgcagc cgacgatcct cgttcattca gcacacaagc
     1141 tgcaggcgat ttgccgcacg acgatcggcc ggttgtgcga aacgtggtcg aacgacggcg
     1201 gccgaacgtg gcagccgctg cgcttgatcg acctgcccaa ctgcaacagc ggcgtggacg
     1261 ccgtcacgct tcgcgatggc cggcacctgc tggtctacaa ccattcgaac ctggaaaaag
     1321 tgcgctatcc gctgaacgtc gcgatcacgc gcgacggcgc cacctggcaa gccgccgcgg
     1381 tgctggagtc agaaccgccc gggcagtatt cgtaccccgc ggtgatgcaa agcctcgatg
     1441 ggctggtgca tatcgcgtac acgttcaagc ggcggaacat caagtatgtc gtgatggatc
     1501 cgagcgcgat cgagccgcag ccgatcgaga tgttcgtctg cccaccaccg gggagtccga
     1561 cgcatcggag cgatgagttt cagtgagtct ctcagtttat cccgagcgaa gcacgtactc
     1621 gtgcgcagtc gaaggatctg gctgaggagt atgtgaagcg ccacgcgcgc catggctgcc
     1681 gcttgcggct cagcgtcttt attcgagaac acatgctaag ccgcaagcgg cgttgccatt
     1741 acggagcttc atagacagag gtcgtcgtcg gatgcgtggt cgccgccgcg tttgaaatcg
     1801 tccacgtgca cgcatcggta agcgtccgtc catcgcgctg accgatcgcc ttcaactcac
     1861 accgcccttc gctcagcatc acgtcgggcc agacaaagac atgatccgcc gccgttaccc
     1921 ggccgagcga ttgtccattc atgaccagct ccaccgtctg gcaattcgaa tacaccttca
     1981 ccgcgcgctt ccccggcggg tgcggcgtgt aacgccgcga cgtgatgtac acgaacggct
     2041 ccggcgacca attcgccttg taaaagtaaa acgcatcctt ccgcgtggcg cgatcggcgg
     2101 tc
//
LOCUS       DATIMH010000475         1897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4151525, whole genome shotgun sequence.
ACCESSION   DATIMH010000475 DATIMH010000000
VERSION     DATIMH010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4151525"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..973)
                     /locus_tag="VLI90_08235"
     CDS             complement(287..973)
                     /locus_tag="VLI90_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSV14234.1"
                     /translation="MPLRRLLEIIGQYGGFFIVAILIVVAGAWAFIALTDAVREGDTG
                     RFDLSIEHYVADHPGPAWLEEVGRDITALGGVAVIALVVLAVIGYLLLRGAYGAMWLV
                     IIATGSGLLLSTALKLMVHRDRPAIIAHRSAVYTTSFPSGHSMLSAVVYLTLGALLAR
                     LAKERVIKFYFLAIALILTGLVGISRVYMGVHWPTDVLAGWTAGLAWAALCWAVTRYL
                     QRRGAVERDS"
     gene            complement(1288..1692)
                     /locus_tag="VLI90_08240"
     CDS             complement(1288..1692)
                     /locus_tag="VLI90_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14235.1"
                     /translation="MSRVAWAAMLAGQLIFFITINRLWNAGRFDPRPDLSRTLFLVNV
                     GVFASVLAAGWFLRRSIARPEIDGMISPRVFARATIVFIALCGLPVLLGVVTMAISLE
                     MWPTIVVPALAFTIQLLNFPSGALVRGVGDRQ"
BASE COUNT          411 a          596 c          602 g          288 t
ORIGIN      
        1 attacatgcg gggcttcgtc cgcagagtag gcgcgttctt atcgcggcgc ctcctgtata
       61 ctgcgctatg gagttagacg gtgcttcact ccagcggcac aggtgctatg gcttcaacta
      121 atgaacgaag aaagccagcc cggcgcaagg ttgtgaacac gtgcgccaaa ccggcgccgc
      181 aggcgagact cttgaagctt gccgagattt atcgtccgcc tcaacgctgg ttcgatggaa
      241 cggattgccc cttcgtacca accaagaagt agaacggcca cggcgtctat gaatcccgct
      301 ccaccgcgcc ccgccgctgc aggtatctgg tcacggccca gcagagcgcg gcccatgcga
      361 gacccgcggt ccagccggcc aggacatccg tcggccaatg cacgcccatg tacacgcggc
      421 tgatgccgac caggccggta aggatcagcg cgatcgccag gaaatagaat ttgatcacgc
      481 gctctttcgc cagtcgcgcc agcaacgcgc caagcgtgag gtagacgacg gcggagagca
      541 tcgagtggcc gctggggaag ctggtcgtat agacggcgga gcggtgcgcg atgatcgcgg
      601 gacgatcgcg gtggaccatt agcttcagag ctgtgctgag cagcagtccg ctgccggtgg
      661 cgatgatgac gagccacatc gcgccgtacg cgccgcgcag caggaggtaa ccgatgacgg
      721 cgaggacgac cagcgcgatg acggcgacgc cgccgagggc ggtgatgtcg cggccgactt
      781 cttccagcca cgcgggaccg ggatgatcgg cgacgtagtg ttcgatggac agatcgaagc
      841 ggccggtgtc gccttcgcgc acggcgtcgg tcagcgcgat gaacgcccag gcgccggcga
      901 cgacgatgag aatggcgacg atgaagaacc cgccgtactg gccgatgatc tcaagcaagc
      961 ggcgaagcgg catgcgggca gcacgttacc gctgcgcgcg cacgcgtgca aagggacagg
     1021 atgcatgatg ccgcgtagcg gtagccccgc aggggcacgt tcgtagcacg ggcgtctcgc
     1081 ccgtgatgcg tgtattcacg cacggcgatg gtacaccatc gacaaagcaa tcgcgcaagc
     1141 gacggtcgtt taaacctcat cgcttgcgcg attgtttcat cgacggcgta tcgtcgatgt
     1201 tcgcggagta ccacgaaaca cgggctggaa gcaccgtgcc acgtaaaaca cgtgcccctg
     1261 cggggctacc gctacgcggc ggaaaattca ttgccgatcg ccaacacctc ggacgagcgc
     1321 gccgctgggg aagttcaaaa gctggatcgt aaacgccagc gccggcacta cgatcgtcgg
     1381 ccacatctcc aggctgatcg ccatcgtgac gacgccgagc aacaccggca ggccgcacag
     1441 cgcgatgaag acgatggtgg ccctcgcgaa aacgcggggc gagatcatcc cgtcaatctc
     1501 gggccgggcg atgcttcgcc gcagaaacca gccggcggcc agcacgctgg cgaagacgcc
     1561 gacgttcacg aggaacagcg tgcgcgacaa atccggccgc ggatcgaatc gcccggcgtt
     1621 ccacaatcga ttgatcgtaa tgaagaagat cagttgcccc gccagcatgg cggcccaggc
     1681 cacgcgtgac atggccaaca taccgcgggg cgagaactcg ctgccgtgcc gcggcgataa
     1741 actctgctgc gtcataaaaa atgtccccgt acacaaacgc ccggctcgcg cgcgtgcaag
     1801 cccaaaaatc gaaaacttgc cgacttgagc gtcaccctaa cagggtgcga acgcgcggcc
     1861 ttccaattgc tccactctca aatacggagc accaacg
//
LOCUS       DATIMH010000476         5122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1554746, whole genome shotgun sequence.
ACCESSION   DATIMH010000476 DATIMH010000000
VERSION     DATIMH010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554746"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VLI90_08245"
     CDS             <1..404
                     /locus_tag="VLI90_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HSV14236.1"
                     /translation="TITGVSEVIKPRKPSFKAIAVEPVTSPVITQTMKHEPVKPGRHK
                     IQGIGAGFVPGNLHLNVVDEVIQITDDEAFEWARRLAKEEGIFAGISSGGNAAAAAKV
                     AARPENKGKLIVTIMCSFGERYLSTPLFEEE"
     gene            553..921
                     /locus_tag="VLI90_08250"
     CDS             553..921
                     /locus_tag="VLI90_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14237.1"
                     /translation="MLSADAEANELEYDRTVIAATRLGPWHVESVDDDLCMNALLVVS
                     EPDEAALEESGNNIAIVLLQSPRYADIKDGRWHQNAAFIARARTRWPEAIEQIDLLNQ
                     QLAALEQENAMLRQQASGND"
     gene            932..1546
                     /gene="cysC"
                     /locus_tag="VLI90_08255"
     CDS             932..1546
                     /gene="cysC"
                     /locus_tag="VLI90_08255"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HSV14238.1"
                     /translation="MSKGKPSNITWHGGAVTREDRQRLLGQRGVTVWMTGLSGSGKST
                     IAVLLEQELIARGRLAYRLDGDNVRHGLNKNLGFNAEDRTENIRRIGEVAKLFTDAGV
                     ITIVSFISPYARDRNAVRAMMNAGDFVEVYVKASVAAAEQRDPKGLYKKARAGEIKGF
                     TGIDDPYEEPANAEVVLETEKQSAVESAAQVLNYLESKRYLAGE"
     gene            1602..2489
                     /locus_tag="VLI90_08260"
     CDS             1602..2489
                     /locus_tag="VLI90_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSV14239.1"
                     /translation="MLQNMQSEFVDLHCHSTASDGTLLPADVVRLAVRSGLSALALTD
                     HDTIGGVAEAAAEAKKLGIDFLPGIEISAEYPSPGTMHILGYGVDPNSASLKRLTDQL
                     IEGRDNRNPRIVAKLNEMNVAVTMDEWENEAKGNVLGRPQLAAILLRKGYVSSIKQAF
                     DKYIGQGAPAYFDKERLTPKRALAMIRESGGLPVLAHPIQLRTTNDGQLDRIVKDLLD
                     LGLAGLEIIHSDHDAAWVEKCTSLADRYGLLKTGGSDFHGTNKKDIELGVARGRRIPR
                     ELFDALTQRLAAGQRTSAG"
     gene            2712..3467
                     /locus_tag="VLI90_08265"
     CDS             2712..3467
                     /locus_tag="VLI90_08265"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HSV14240.1"
                     /translation="MTTEIAEQTDVDLESLNAIFETADAARMVEWAVAQFGAEHTVMS
                     SSFGAESAMLLHMATRVAPDMKIIFVDTGYLFPETHLFMEQLRRRLNLNVWAYRTMQD
                     PIAWLAKNGEADPAIRNDRNICCAVNKNEPFERAMAELRPKAWLRGIRRDQASTRKDR
                     QFVEWSRRFNCYAISPMLKMTRRDIHAYLKQHDLPYHPLFDKGYASIGCNPISCTRPI
                     QIGEDARAGRWAGSDKIECGINVDNNSLDSAQI"
     gene            complement(3644..3904)
                     /locus_tag="VLI90_08270"
     CDS             complement(3644..3904)
                     /locus_tag="VLI90_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14241.1"
                     /translation="MQGSDKPIVPLPADAYVFIDGTPRFQQQNFTNLEGTFIVDVPLQ
                     TTDRYLTLVTCTHADPKIANSIWFNWIVFSDPQFQLSANAPR"
     gene            complement(4333..4406)
                     /locus_tag="VLI90_08275"
     tRNA            complement(4333..4406)
                     /locus_tag="VLI90_08275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4370..4372),aa:Arg,seq:cct)
     gene            complement(4440..4769)
                     /locus_tag="VLI90_08280"
     CDS             complement(4440..4769)
                     /locus_tag="VLI90_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSV14242.1"
                     /translation="MPVEKWSDNVVVAHLADDPQFTEDLLSIEDSTEAGKTDVVLDFA
                     AVHFVNSSNLARLLKLRKQMSSAERRLVMCSINTQVWGTFLVTGLDKVFEFSDNVPTA
                     LATLQIA"
BASE COUNT         1083 a         1517 c         1606 g          916 t
ORIGIN      
        1 gcacgatcac cggcgtgtct gaagtgatca agccgcgcaa gccaagcttt aaggcgatcg
       61 cagtagaacc agtcacttcg ccggtcatca cgcagacgat gaagcacgaa ccggtgaagc
      121 ccgggcggca taagatccaa ggcatcggcg ccggcttcgt gccgggcaac ctgcacctga
      181 acgtcgtgga cgaggtgatt cagatcaccg atgacgaagc cttcgaatgg gcgaggcgac
      241 tggcgaagga agaaggcatc ttcgccggca tcagctctgg cggaaacgcc gccgccgccg
      301 ccaaggtcgc ggcgcgaccg gagaacaagg ggaagctgat cgtgacgatc atgtgcagct
      361 ttggcgaacg gtatctctcg acgccgttgt ttgaagagga gtgaccggag cttcggcgtc
      421 aacgcattat ctccaccacg agagatgtgc ggattaagta gcatgggcgt cgcgcccgtg
      481 ccagaggact tcgacgcgag cgagacgccc gtgctacctt caggcgttgc tgcgcagtga
      541 gaggggccac aaatgttgtc cgctgacgct gaggcgaatg agcttgaata cgaccggacg
      601 gtgatcgcag cgacccgtct gggcccgtgg cacgttgaat ccgtcgatga cgacctgtgc
      661 atgaacgcgt tgctcgtcgt gtcagagccc gatgaagccg cactggaaga atcgggcaac
      721 aacatcgcga tcgttttgct gcagtctccg cgctacgcgg atatcaagga cggaaggtgg
      781 caccagaacg ctgccttcat cgctcgcgcc cgcacgcgtt ggccggaagc gatcgagcag
      841 attgatctgc ttaatcagca gttggccgcg ctggagcagg agaatgccat gctccgacag
      901 caggcgagcg gaaatgactg aggtaatttc gttgtctaaa gggaagccat ccaacatcac
      961 ctggcacggc ggagccgtca cgcgcgagga tcgccaacgc ctgcttggtc agcgtggggt
     1021 cacggtgtgg atgaccggct tgagcggctc cggcaagagc acgatcgccg tgctgctgga
     1081 acaggagctg atcgcgcgag ggcgcctggc gtatcgcctc gacggcgaca acgtccgcca
     1141 cgggttgaac aagaatctgg gcttcaacgc ggaagaccgg acggagaaca tccgccggat
     1201 tggcgaggtg gccaagctgt tcaccgatgc cggcgtgatt acgatcgtca gcttcatcag
     1261 cccctacgcc cgcgaccgca atgcggtgcg ggcgatgatg aatgcaggcg atttcgtcga
     1321 ggtgtacgtg aaggcgtcgg tcgccgccgc ggaacagcgc gatccgaagg gactctataa
     1381 gaaggcccgc gcgggcgaga tcaaaggttt caccggcatc gacgatccgt acgaggagcc
     1441 ggcgaatgcg gaggtggtgt tggagacgga gaagcagtcg gcggtggagt cggcggcaca
     1501 ggtactgaat tatttggaat cgaagcggta tctggcgggc gagtaacgtg gcacgggcgt
     1561 ccggcccgtg ccaaggtgcc ccggacacgg gcgagacgcc cgtgctacag aatatgcaaa
     1621 gcgaatttgt cgatctccac tgccactcca ccgcgtccga cggcacgctg ctgccggccg
     1681 atgttgtgcg cctcgccgtt cgcagcgggc tcagcgcgct cgcgctcacc gatcacgaca
     1741 cgatcggcgg tgtcgccgaa gcggcggccg aggcgaagaa actcggcatc gacttccttc
     1801 ccggcatcga aatctccgcg gaatatccgt cgccggggac gatgcacatc ctcggttacg
     1861 gcgtcgatcc gaacagcgca tcgctcaagc gtttgaccga tcaactcatc gaaggccgtg
     1921 acaaccgcaa cccgcggatc gttgcgaagc tgaacgagat gaacgtcgcg gtgacgatgg
     1981 acgaatggga gaacgaagcc aaaggcaacg tcctcggccg gccgcagctg gcggcgatcc
     2041 tgcttcgcaa ggggtacgtc agctcgatca aacaggcgtt cgacaaatat atcggccaag
     2101 gcgcgccggc ctacttcgac aaagagcgcc tgacgcccaa gcgcgcgctt gcgatgatcc
     2161 gcgaatccgg cgggcttccc gtcctcgccc atccgattca attgcgcacc accaacgacg
     2221 gccagctcga tcgcatcgta aaggacctgc tcgacctggg cctggccggc ctcgaaatca
     2281 tccacagcga tcacgacgca gcgtgggtcg agaaatgtac cagcctcgct gaccgctatg
     2341 ggctattgaa gaccggtggc agcgattttc acgggacgaa caagaaggac atcgagctcg
     2401 gcgtcgcccg cggccgccga atcccgcgag agttgtttga tgcccttacc caacgcctcg
     2461 cggctggaca acgaacgtct gctggatgat tgacggcgaa gcgtttcacc gccgagtcac
     2521 ggagacacag aggaagcagt aagagagttg aaccatagat ttcgcaaatt gcgcagattc
     2581 agaacgagct ctctcttcat ctgcgaaatc tgcggttaaa actcttcctc tggtgtgcct
     2641 ccgtgccccg gtggtcaatt acgcttgcgg cggcggaagg atcagaaatc gtgcctatta
     2701 ttgctgaacc gatgacgact gaaatcgctg aacaaaccga cgtggatctc gaatcgctca
     2761 acgcgatatt cgagaccgcc gacgccgcgc gcatggtcga atgggccgtc gcccaatttg
     2821 gcgccgagca caccgtcatg tcctccagct tcggcgccga gtcagccatg ctgctgcaca
     2881 tggcgacgcg cgtcgcgccg gacatgaaga tcatctttgt tgacaccggc tatctgttcc
     2941 ccgaaacgca cctgttcatg gagcagctcc gccggcggtt gaacctgaac gtgtgggcat
     3001 atcgcacgat gcaggacccg atcgcctggc tggccaagaa cggcgaagcc gatcctgcta
     3061 ttcgcaacga tcgcaatatt tgctgcgctg tgaacaagaa cgaaccgttc gagcgcgcga
     3121 tggccgagct tcgaccgaag gcctggctgc gcggcatccg ccgcgatcag gccagcaccc
     3181 gaaaggaccg ccagttcgtg gaatggtccc gacgtttcaa ctgctacgca atctcgccga
     3241 tgctgaagat gacccggcgg gacatccatg catatctgaa gcagcacgac ctgccgtacc
     3301 atccgctgtt cgacaaaggc tacgcgtcga tcggctgcaa cccgatcagc tgcacccgcc
     3361 cgatccagat cggcgaagac gcccgagccg gccgctgggc gggcagcgat aagatcgagt
     3421 gcgggatcaa cgtagacaac aactcattgg attcggcgca gatctaattc cgggtgtagc
     3481 gcaagacgcc aaggaagcaa aggaaaatcg cttttttcgt agcatgggcg tctcgcccgt
     3541 gccgaggcgc caggcacagc gagacgtccg cgcgctacgc cgggtgcttc accattgggc
     3601 cttcttcctt ggtgtcttgc tttatccgtt cagagatgag gactcaacgt ggagcattcg
     3661 cgctgagttg gaactgcggg tcgctgaaga cgatccagtt gaaccagatc gagttcgcga
     3721 tcttcggatc ggcgtgggtg caggtcacga gcgtcaggta gcgatcggtt gtctgcagtg
     3781 gaacgtccac gatgaaggtg ccttcgaggt tggtgaagtt ctgctgctgg aaccgggggg
     3841 tgccgtcgat gaagacatac gcgtcggcgg ggagggggac gatgggtttg tcgctgcctt
     3901 gcacggcgcg ctggtcgatg caatgcgtga ggagacgtcg agcgattacg tgatcacccg
     3961 ataccgctcg cccaactgca accgccgcac tcacttcgaa cggatcatcc gcaacgccgg
     4021 cctcacgccc tagccgcagc tgtggcacaa catgcggtta acccagcaga ccgagctcgc
     4081 cgacaagtac cctatccacg tcgtctgtgc gtggctgggc aacagccggg cggtggctca
     4141 agagcattac ctgcagttga ccgacgtgca tttcgagcat gcggcgggcg tgaaggattt
     4201 gagtcagggc ggggcgcaga atccggcaca gtcactgtcc accccgagca atcccgagca
     4261 ttcagccacc gggcagaatc agcaaaatca ggggaaaaac agcgatgagg gcgaactgtg
     4321 gggagtggtc agtacccgcg acaggattcg aacctgtgac ctccggttta ggaaaccagc
     4381 gctctatcca actgagctac gcgggcgata agcgcgcgac gagcacacgc ggcttgattt
     4441 taggcgatct gcagtgtcgc cagcgcggtc gggacgttgt cgctgaactc gaagacttta
     4501 tcgaggccgg ttaccaggaa tgtgccccag acctgcgtgt tgatgctgca catgaccagc
     4561 cggcgttcgg cgctgctcat ctgcttgcgc agcttcagca gccgggccaa gttcgacgaa
     4621 ttgacgaaat gcaccgcggc gaagtccagc acgacgtcgg tcttgccggc ttcggtggaa
     4681 tcttcgatcg acagcaggtc ctcggtgaac tgcggatcgt ccgccaggtg agccacgacg
     4741 acgttgtccg accatttctc aacgggcatg cgtgcatcct ccagcggacc gcgaacgaca
     4801 cagcgccccc gcacggctgg gcgagacaac tacggtaccc atccgtggca tcgttggcaa
     4861 cgcaaagccg cccctcgacg gcggcgtcgg gctacgccga agaccaagat tcggagtcga
     4921 atacacgcat cttccgtcat tctgcattca ccattctgca tcactactgt ccggttagtc
     4981 accggcgtta ggttgggttg acctcggttt tgccgagtcg cagaactccg atgttagggt
     5041 acacgatttc aatttcgttg gtcgattttg ccgtcgcggc caggtggctt ggccgacgat
     5101 cgggcgatga acaccggtcg ca
//
LOCUS       DATIMH010000477         5182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3575875, whole genome shotgun sequence.
ACCESSION   DATIMH010000477 DATIMH010000000
VERSION     DATIMH010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3575875"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VLI90_08285"
     CDS             <1..281
                     /locus_tag="VLI90_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14243.1"
                     /translation="NSAWWGIPFALAGAAQQRYLAAGPVIRTALANQIRGELRGGAEL
                     DLHNATSTPVYQPGMSGRLCQTRGCGSRLPSVANYCPRCGASATQRSA"
     gene            298..447
                     /locus_tag="VLI90_08290"
     CDS             298..447
                     /locus_tag="VLI90_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14244.1"
                     /translation="MYIILAAVGWAWFAIAGSYLWFRLRPRIAPPGLSIDTIETSSTE
                     AVRDE"
     gene            444..1955
                     /locus_tag="VLI90_08295"
     CDS             444..1955
                     /locus_tag="VLI90_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14245.1"
                     /translation="MSTTNAEPSIHRQREQDFIRHVERLLEDDRLRVDTTRGRRPVSS
                     CMTKVDRTDRAVDLKRVMAEMNRPDRDLQNQMPVGETLDVTLAQRRLMVLRTVVGQLK
                     VVCVSPTRSLIAGQDPQPLKPAEVQKLLGEVPPSTNGVPSTVVLMSTSGFTLEAHELA
                     VRRADRTVVMVEPNKAGGWAVYGPVETKALVDLFDPEAEDSKRSRVREEIESAKAELT
                     GSGIAGDRIASKTQLPLQLVEAELKAYAKANTGLLAKRLDGRVVLFREGSVPTASNAS
                     ASAGGGGEMPLIDRIKTLFARKGENEKKIAFLSERRTALSQQRDRSYEDMSNLEQQEA
                     ALRRSFKEATGDITKRRVTSQLLQLRKEIERRQQLLAVLNQQINVVSTHLHNLELVQQ
                     GQGAKLPDSEEVAADAAKAEEMLAQLEADNELAASVGGITSGAGMSAEEQALFEELER
                     ESGGGSAAEKQASEREQSPAMTSTTTPRNTSREPIAAPSPAAPEQRRAEPEAG"
     gene            2041..2658
                     /locus_tag="VLI90_08300"
     CDS             2041..2658
                     /locus_tag="VLI90_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14246.1"
                     /translation="MALIDKLFNRKKTLSQLDKQELRKEEILLTKQRDRLFKRIETIG
                     AQKQQIFKQGATQKSPELRKALAQDFELKTQEQLMAARELNLRSKELLTVSRLRMVRE
                     NNDRGRALGRLNLTDKDVAKISGLLEDDNVSQDLYRDRLDALLEIGAQGDKDALATAG
                     LSSAGQELMNIWDELDRGSVKHEQAFEQADQAVRRKAAAADGEGG"
     gene            2914..3216
                     /locus_tag="VLI90_08305"
     CDS             2914..3216
                     /locus_tag="VLI90_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14247.1"
                     /translation="MNTVYVDKTMERDVLPPPQASAALDYASPGIRAKPPFDFAEALR
                     QATFALGVGLLVFGITVTWGSERAWSENIGAFTGIGAGLIALTISWPGRLGRRRGD"
     gene            3225..3395
                     /locus_tag="VLI90_08310"
     CDS             3225..3395
                     /locus_tag="VLI90_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14248.1"
                     /translation="MPQLHRNNAAEWIMLIALSPFVWMRRLFSRPRTSDPATPRPPRR
                     VMIEGLPPRTMN"
     gene            3434..4042
                     /locus_tag="VLI90_08315"
     CDS             3434..4042
                     /locus_tag="VLI90_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14249.1"
                     /translation="MGLFKSKDERRIEREMKIRAGLRSIERSIRQQEKFADDFIKNAQ
                     HAKKIGDDGQYNFIRSALKRTAAVKKMLERQLLSMKNAMLIQQQAQASQQFAESMNLM
                     AGEIGRIFGEMDLTQTQAQWERAVSQAGSLEERMDLFLDSMEQSATSATPTSAKEDLV
                     SDEEIDRMIQADVLAAEKTELGKLDQLESEIAKELGATKQKD"
     gene            complement(4176..4808)
                     /gene="acpS"
                     /locus_tag="VLI90_08320"
     CDS             complement(4176..4808)
                     /gene="acpS"
                     /locus_tag="VLI90_08320"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HSV14250.1"
                     /translation="MLPETWEIHEAEIDELDILGGDKLEDIAGGHCVSDAVVARALLC
                     APARMAVVWLLPFERPSYRRGAPPPIERRQASAYAYAMPIVGHGIDIVETARIKNMVD
                     EHGQRFLDRCFTPDEQAYCARSAKRYFEHLAGRFAAKEAVLKVLGTGWRGGIAWTDIE
                     VIKEESGQPRIALTGECARIAKELGIAQWHISLSHIETHATASAIGLRGA"
     gene            4782..>5182
                     /locus_tag="VLI90_08325"
     CDS             4782..>5182
                     /locus_tag="VLI90_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HSV14251.1"
                     /translation="MDFPGLWQHTTCPISELTLDSFDHGFGFDGSSIRGWQAINESDM
                     LLVPVADTAKIDPFLQHTTLSIICDVRDPITKKEYSRDPRSIARKAVAYLKKSKIADS
                     ARFGPELEFFIFDQAFYDQGINFSKHYVGSR"
BASE COUNT         1061 a         1563 c         1705 g          853 t
ORIGIN      
        1 cgaactccgc gtggtggggt attcccttcg cgctggccgg tgcggcgcag caacggtacc
       61 tggcagccgg gccggtgatt cgcacggcac tggcaaacca aatccgcggt gagcttcgcg
      121 gcggcgcgga acttgatcta cacaatgcga cctcgacgcc ggtatatcag ccgggcatga
      181 gcggccggtt gtgccagacg cgcggctgcg gctcgcgtct accatcagta gccaattact
      241 gtccgcgttg cggcgcatcg gcgacgcagc ggagcgcgtg accaatcgat gcgcacgatg
      301 tacatcattc tcgccgcggt tggttgggcg tggttcgcca tcgcggggag ttatttgtgg
      361 tttcgcctgc ggccgcggat tgcgccgccg ggattgtcga tcgacacgat cgagacgagc
      421 agcacggagg ctgttagaga cgaatgagca cgacgaacgc cgaaccatcg attcaccgcc
      481 agcgcgagca ggacttcatc cggcacgtcg agcggttgct ggaagacgac cgcctgcgcg
      541 tggataccac ccgcggccgc cggccggtca gctcgtgtat gaccaaggtc gatcgcaccg
      601 atcgggcggt ggacctgaag cgcgtgatgg ccgagatgaa ccggcccgat cgcgacctgc
      661 aaaaccagat gccggtcggc gaaacgctgg acgtaacgct ggcgcagcgg cggttgatgg
      721 tgctgcgtac ggtggtcggc cagctgaaag tcgtatgcgt ctcgccgacg cgctcgctga
      781 ttgccggtca agacccgcag ccgttgaagc ccgcggaagt gcagaagctg ctgggcgaag
      841 tgccgccgtc caccaatggc gtgccgtcaa cagtggtgct gatgtccacc agcgggttca
      901 cgctcgaagc gcacgagctg gccgtgcggc gggcggatcg cacggtcgtg atggtcgagc
      961 ccaacaaagc cggcggctgg gcggtgtatg ggccggtgga aacgaaggcg ctggtcgatc
     1021 tattcgatcc cgaagcggaa gattccaagc gcagccgggt gcgcgaagag atcgaatcgg
     1081 cgaaagccga gttgaccgga tccggcatcg ccggcgatcg gatcgccagc aaaacgcagc
     1141 tgccgttgca gctggtggaa gcggagttga aagcatatgc caaggcaaac acaggcttgc
     1201 tggccaagcg gcttgacggc cgcgtggtcc tgtttcgcga aggcagcgtg ccgacggcgt
     1261 cgaacgcatc ggcgtcggcc ggcggaggtg gcgagatgcc gctgatcgat cgcatcaaaa
     1321 cgctcttcgc ccgcaagggt gagaacgaga aaaagatcgc gtttctgtcc gagcgcagaa
     1381 cggcgctcag ccagcagcgc gaccgttcgt acgaggatat gagcaacctg gaacagcagg
     1441 aggcagcgct tcgtcgatcc ttcaaggaag cgaccggcga catcaccaag cggcgcgtga
     1501 ccagccagct gcttcaactc cgcaaggaga tcgaacgtcg ccagcagttg ctggcggtgc
     1561 tgaaccagca gatcaacgtc gttagtacgc acctgcacaa cctggagttg gtgcagcagg
     1621 gtcagggcgc gaaactgccc gacagcgaag aagtcgcagc cgacgccgcc aaggccgaag
     1681 agatgctggc gcagctggag gccgacaacg agctggccgc cagcgttggt gggattacga
     1741 gcggcgccgg gatgagcgct gaagagcagg cgttgttcga agagttggaa cgcgaaagcg
     1801 gcggcggcag cgcggcggag aaacaggcga gcgagcgaga gcaatcgccg gcgatgacgt
     1861 caacgacgac gccgcgcaat acgtcgcgcg agccgatcgc cgcaccgtcg cccgccgcgc
     1921 cggaacaacg tcgagccgag cccgaagcgg gctagccttt gtggtcccct cgcccacgta
     1981 ctcgtgacgt attcgtggga gagggttagg gtgattcagg attcacgacg ttcccaacaa
     2041 atggcactca tcgacaaact cttcaaccgc aagaagaccc tcagccagct cgacaagcag
     2101 gagctgcgca aggaggagat cctgctcacc aagcagcgcg atcggctctt caagcgcatc
     2161 gaaacgatcg gcgcgcagaa gcagcagatc ttcaagcagg gcgcgaccca gaaatcgccg
     2221 gaactgcgca aggccctcgc gcaggatttc gaactgaaga cgcaggaaca gctgatggcg
     2281 gcgcgcgagc tgaacctgcg cagcaaggag ctgctcacgg tcagccgcct gcgcatggtc
     2341 cgcgagaaca acgaccgcgg ccgcgcgctg gggcgattga atctcaccga taaagatgtc
     2401 gccaagatta gtgggctgct cgaagacgac aacgtcagcc aggatttgta tcgcgatcga
     2461 cttgatgcgc ttctggaaat cggcgcgcaa ggcgacaagg acgccctggc aacggctggg
     2521 ctgtcatcgg ccggccagga gctgatgaac atctgggatg aactggaccg tggcagcgtg
     2581 aagcacgaac aggcgtttga gcaggccgac caggccgtac gccgcaaagc cgccgccgcg
     2641 gatggggagg gcggatgaac gttcgccaca cgctggagta tcagccaatt ccgacgccgt
     2701 ctcggggcca cgtaaccggc gcgcatgccg ttgcgatgct actttgcctg ggtgcttacg
     2761 tcgcctcggt ctgggcagga ggaaagtggg ggggagagga tggcggttgg tttttgcttc
     2821 ctggtttttt gtcgtttgcc gggatctttg taattccagt atgcgcgatc acccgaagac
     2881 ccagaagccc ggaataaagc ggaagtcagt gacatgaata ccgtgtatgt ggacaagacg
     2941 atggagcgcg acgtgctacc gccgccgcaa gcgtcggcgg cattggatta cgcgtcgccc
     3001 ggcatccgag ccaaaccgcc gtttgatttc gccgaagcat tgcggcaagc gaccttcgcg
     3061 ctcggcgttg gtttgctcgt cttcggcatc accgtcacct ggggcagcga gcgcgcgtgg
     3121 agcgagaaca tcggcgcatt caccggcatc ggcgccggcc tgatcgcctt gacaatttca
     3181 tggcccggcc gcctcggccg ccggcgcggt gattgagatt tacgatgccg cagcttcatc
     3241 gcaacaacgc cgccgagtgg atcatgctga tcgcgctgtc gccgttcgtc tggatgcgga
     3301 ggctgttcag ccgtccacgt acgagcgatc cggcgacgcc gcgtccgcca cgacgggtca
     3361 tgatcgaagg tctgccgcct cggacgatga attgatggag cacgggcgag acgcccgtgc
     3421 tacaaaggat gcaatgggcc ttttcaagag caaagacgaa cgccggatcg agcgtgagat
     3481 gaagatccgc gccggcctgc ggtcgatcga gcgttcgatc cgccagcagg aaaagtttgc
     3541 cgacgacttc atcaagaacg cgcagcacgc caagaagatc ggcgacgacg ggcagtacaa
     3601 cttcatccgc tccgccctga agcgcacggc tgcggtgaag aagatgctcg agcggcaact
     3661 gctgtcgatg aagaacgcga tgctgatcca gcagcaggcg caggccagcc agcagttcgc
     3721 cgagagcatg aacctgatgg ccggcgagat cggtcgaatc ttcggggaga tggacctgac
     3781 gcaaacacag gcgcagtggg aacgggccgt ctcgcaggcg gggtcgctcg aagagcggat
     3841 ggatctattc ctggacagca tggagcagtc ggcgacgtcg gcgacgccga ccagcgcgaa
     3901 ggaagacctc gtcagcgacg aagagatcga ccgcatgatc caggccgacg tcctggccgc
     3961 ggaaaagacg gagctgggga agctcgacca gctggagagc gagatcgcga aggaactcgg
     4021 ggcgacgaag cagaaggatt gatccgctcg atctgcgcct ccatcgcagc cgcggggctt
     4081 cagcccgcgg accgtggaga tcgcgagcgg tgctaatagt tcgaacggtc cgcgggctaa
     4141 agcctcgcgg ctgcgacgga cgcgcacctc ttgaactacg cgccgcgcaa tccaatcgcg
     4201 ctcgcggtcg cgtgggtttc gatgtggctt aaactgatgt gccactgcgc gattccaagt
     4261 tccttggcga tgcgcgcgca ttccccggtc agcgcaatcc gtggttggcc gctctcttcc
     4321 ttgatcactt cgatatccgt ccacgcaatc cctccgcgcc agccggtgcc tagcactttt
     4381 agcaccgctt ccttcgcggc gaatcgtccg gctaaatgct cgaaataacg cttggcgctg
     4441 cgggcgcagt acgcctgttc gtccggcgta aagcagcggt ccaggaagcg ctggccgtgc
     4501 tcgtccacca tgttcttgat gcgagccgtc tcgacgatgt cgatgccatg tccgacgatg
     4561 ggcatggcat aagcgtacgc gctcgcttgc cgcctctcaa ttggcggcgg tgcgccccgc
     4621 cggtaggatg gccgttcgaa gggaagcaac caaacgaccg ccatccgcgc aggcgcgcac
     4681 agcagcgctc gcgccactac agcatcggag acgcaatgac cccccgcgat gtcctcaagc
     4741 ttgtcaccac caagaatgtc cagttcatcg atctccgctt catggatttc ccaggtctct
     4801 ggcagcacac gacctgcccg atcagcgagc tgacgctcga cagtttcgat cacggtttcg
     4861 gcttcgacgg ttcatccatc cgcggctggc aggcgatcaa cgaaagcgac atgctgctgg
     4921 tccccgtggc cgacacggcc aagatcgatc cgttcctgca gcacacgacg ctgtcgatca
     4981 tctgcgacgt gcgcgatccg atcaccaaga aggaatacag ccgggatccg cggtcgatcg
     5041 cccgcaaggc ggtggcctat ttgaagaaga gcaagatcgc cgattccgcg cgcttcggcc
     5101 cggagttgga gttcttcatc ttcgaccagg cgttttacga ccaggggatc aacttctcga
     5161 agcactacgt cggcagccgc ga
//
LOCUS       DATIMH010000478         4878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1555316, whole genome shotgun sequence.
ACCESSION   DATIMH010000478 DATIMH010000000
VERSION     DATIMH010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555316"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VLI90_08330"
     CDS             complement(<1..484)
                     /locus_tag="VLI90_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14252.1"
                     /translation="MVATAGTTIDPRQIQGIKRLRHLLPLLAPLHDVGCARDTAGNRE
                     LHLDEYVTLVLLYLMNPLIDSVRALQHAAAIDKVSEQLRVKRFSLGSFSESVRSFDPQ
                     KLKEVIAQLSGGLKPVNVDPRLKTHLDHAFTIRRRHRVGCDRQRDRGDVAAVQRWQRQ
                     A"
     gene            741..1331
                     /locus_tag="VLI90_08335"
     CDS             741..1331
                     /locus_tag="VLI90_08335"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HSV14253.1"
                     /translation="MDSIRSYFRNIYDNVTSLSIGMKITLKYCFQKTVVVQYPEQKLS
                     FAPRYRGIHEFEADKCIACDQCAKACPVDCIYIDKTAPRKIDKRSGTVNMSDPKNGQL
                     LRFAIDYSKCLFCALCTDPCPTECIHMGKLHDLSSYTREDVVVEFAELAKKNMRTPIP
                     LWMERNADQIPWVKAELDRVKSGTGATTAADIPVVK"
     gene            1568..2962
                     /locus_tag="VLI90_08340"
     CDS             1568..2962
                     /locus_tag="VLI90_08340"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSV14254.1"
                     /translation="MALGKRKPVQQPLLVSTVDLNIRCHPFYEAVNKVLDAHQFDPFV
                     QGLCAGFYDDGSRGGRPGLAPGIYFRCLLVGYFEGIDSERGIDWRCNDSLSLKAFLGV
                     AVDACAPDHSTISRTRRLIDLETHGEVFRYLLKVLANHGLIDGQTVGMDSTTLEANAA
                     MRSIVRRDSGEGYQQFLERLAKESGIETPTREDLAKLDKQRKNKASNDDWQNPHDPDA
                     RITKMKDGTTHLAHKAEHAVDMGEAGHGAILAVNICDAAAGDTATITDTIVQTTENLA
                     ALKDDERVAERVSDQWMSEAVLDKGYRSKQTLLDLQEMTIRSYASEPDRGRQNWDGQP
                     DARDAVYANRRRIKGTRGKRLLRGRGEKLERSFAHCYETGGMRRLYLRGRDNIAKRTL
                     IHDAGFNLGLLMRVSYGLQKPRSLCKAAGALIFALIQWLWAIAMAMVGTRSTKRFQRA
                     DPAVGPRSPTLCAA"
     gene            3203..3583
                     /locus_tag="VLI90_08345"
     CDS             3203..3583
                     /locus_tag="VLI90_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14255.1"
                     /translation="MIPPFPVTFFMRGHGRMYGMKMTGKAFDLMLGSKKCWEYEVTDA
                     VVEADSVSIDWDEGDGTCHLNARSTDHGLTWEAIWGDPGIKLGWTMTLTQYVARSGEI
                     VLWGDWIGESGSIGKMMFQLSPTP"
     gene            3733..>4878
                     /gene="argJ"
                     /locus_tag="VLI90_08350"
     CDS             3733..>4878
                     /gene="argJ"
                     /locus_tag="VLI90_08350"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HSV14256.1"
                     /translation="MPDLHLLSPRGFTAAGVKAGIKSSGKRDVGLLLCEALATAAAAF
                     TTSKVVSPAVIVGQSHVARGRLRGVVVNAGNANACTGRQGLRDAQRMCALASTIGNCT
                     AEQILPSSTGIIGQLLPMEKVEAGITAAGRALGSSAEHAEAFMDAILTTDTRRKSAGV
                     EFKIGRDVVRLAGVCKGSGMIGPRLGVNKANAKPQAAHATMLAYLTTDAAVAAPLLRK
                     LLQNAADASFNAVTVDDHASTNDTACLLASGTSGVKIASPAAIKKFAAALDEVSQSLA
                     YQIAADGEGATKVVKIVVTGATTTAAARAIARTIANSPLVKCAMNGNDPNWGRIVSAA
                     GYAGAAFDANKSSLKLQGTTVFKSGQPVPFDAAKVSEALKLPEVVAEL"
BASE COUNT          999 a         1554 c         1493 g          832 t
ORIGIN      
        1 gtgcttggcg ctgccatctt tgaacggcag ccacatcgcc tcggtcacgt tggcgatcgc
       61 atccaacacg gtgccgtcga cgaattgtga acgcgtggtc caggtgggtc ttcagccgcg
      121 ggtcgacatt gaccggctta agcccgcccg acagctgcgc gatgacctct ttcagcttct
      181 gcggatcgaa cgaccgcacc gactcgctga acgagccaag gctgaaccgc ttgacgcgga
      241 gttgctcgct gaccttgtcg atcgccgccg cgtgctgcag cgcccgcacc gagtcgatca
      301 gcgggttcat caggtacagc agcaccagcg tgacatactc atcgaggtgc aactcgcgat
      361 tgccggcggt gtcccgcgcg cagccgacat catgcagcgg cgccagcaga ggcagcagat
      421 ggcgcaggcg tttgatgccc tggatctgtc tcgggtcgat cgtcgtgcca gccgtcgcaa
      481 ccactgccgg ccattatgcc caagcgctcc agagagcgca atcgcaatat tatgatattc
      541 gtagaagata caactggatc gtcaatggcg tgccgaacag gattggagaa ttcgtcttcc
      601 tcaactcggt cgttcctccg cggcctccgc gtctctgcgt tcaacatctc ttctgccacc
      661 gcgaaagtcg caccggggcg cgggagtgga ctaaaagccg gcgtacgcaa taatggcctc
      721 ggcgcgcgag gcacaatttc atggattcca tccgcagcta ctttcgtaac atctacgaca
      781 acgtcacatc cctcagcatc gggatgaaga tcacgctcaa atactgcttc cagaaaaccg
      841 tcgtcgtgca gtatcccgag cagaagcttt ccttcgcccc gcgctatcgc ggcatccacg
      901 aattcgaagc cgacaaatgc atcgcgtgcg accagtgcgc caaggcctgc cccgtcgact
      961 gtatttatat tgataagacc gcgccgagga agattgacaa acgatccggg acggtaaata
     1021 tgtcagaccc aaagaacggc caactgctgc gcttcgcgat tgactacagc aaatgcctgt
     1081 tctgcgcgct atgcaccgac ccgtgcccga ccgaatgcat tcacatgggc aagctgcacg
     1141 acctgtcgtc atacacgcgc gaggacgtgg tggtggagtt cgccgagctg gccaaaaaga
     1201 acatgcgcac cccgatcccg ctgtggatgg agcggaacgc ggatcagatc ccgtgggtga
     1261 aggcggagct cgatcgcgtg aagtccggca ccggcgcgac gacggcggcg gatattcccg
     1321 tcgtgaagtg atcgcgctcg aagtctttgg cccaaatcgt caggctgccc gccgccgccg
     1381 ccgcgttccc gcgtcggcct tgctcgatat gtccagcctc gccccgcccg tcttgtatgt
     1441 cgtggcaatg gtcaagacga acatctcgtg tgtcgcctag cagcccgtgg cgaaaccaac
     1501 ttatcgactg gaatgcatga gcagaccgcg ctaatctgcg gcgactcgtc aaggagggca
     1561 tcagcccatg gccctcggta agcgaaagcc cgtccagcag ccgctgttgg tcagcaccgt
     1621 cgatctgaac atccgctgtc acccgtttta cgaggcggtg aacaaggtgc tggacgccca
     1681 ccagttcgat ccgttcgtcc aagggctgtg cgccggcttc tatgatgacg gcagccgtgg
     1741 tggtcggccg gggctggctc cgggcatcta cttccgctgc ctgctggtgg gctacttcga
     1801 ggggatcgac tccgaacgcg gcatcgactg gcgctgcaac gacagcctct cgctcaaggc
     1861 gttccttggc gttgccgtcg acgcctgtgc cccggaccac tcgaccatca gccgcacgcg
     1921 aaggctgatc gacctggaga cccacggcga ggtgttccgc tacctgctga aggtgctggc
     1981 caaccatggt ttgatcgacg ggcagacggt ggggatggac tcgaccacgc tggaggccaa
     2041 cgccgccatg cgcagcatcg tgcggcgcga cagtggcgag ggatatcagc agttcctgga
     2101 gcggctggcc aaggagagcg gcatcgaaac gcccacgcgg gaagacttgg ccaagctcga
     2161 caagcagcgc aagaacaagg cgagcaacga cgactggcag aacccgcacg atccggatgc
     2221 caggatcacc aagatgaagg acggcacgac gcacctggcc cacaaggccg agcatgcggt
     2281 ggacatgggc gaagccggtc acggcgcgat cctggcggtg aacatctgcg acgcggcggc
     2341 gggcgacacc gcgacgatca ccgacacgat cgtgcagacc accgagaacc tggcggcact
     2401 gaaagacgac gagcgcgtgg ccgagcgggt cagcgatcag tggatgagcg aggcggtgct
     2461 ggacaaaggc tatcgcagca agcagacgct gctcgatctg caagagatga cgatccgcag
     2521 ctacgccagc gagcccgatc gggggcggca gaactgggac ggccagcccg acgcccgcga
     2581 tgcggtctat gccaaccgaa ggcgcatcaa aggcacacgc ggcaaacgcc tgttgagggg
     2641 ccgcggcgag aagctggaac gctccttcgc ccactgctac gagaccggcg ggatgcgacg
     2701 gttgtacctg aggggccggg acaacatcgc caaacggacg ctgatccacg acgccggctt
     2761 caacctcggg ctgctgatgc gcgtgagcta tggcctgcag aagcccagga gcctttgcaa
     2821 ggccgccggc gcgctcattt ttgctctgat ccagtggctc tgggcgatcg cgatggcgat
     2881 ggtcggcacc aggagcacca aacggttcca acgggccgat ccagcggtcg gtccgcgttc
     2941 gccaaccctc tgcgccgcgt gagcgaaatc acgcgttccg ccacgggctg aataggttca
     3001 tcgcctcgaa gcgttccgag tagtttaaac accgtctcgt ctcggcgcaa caaagcggat
     3061 tctccgaggg gcgatgttac aattcgtccc tcttgccgac cgtccaaaaa gcgaatcatc
     3121 tctcgcgata acagggggat gattgcacga tcgaccagcg actaccttaa ggggcgacca
     3181 tttccagttt ccgagagcaa cggtgattcc accgtttccg gtgacctttt ttatgcgcgg
     3241 gcacggtaga atgtacggca tgaagatgac aggaaaagcc ttcgatttaa tgcttggaag
     3301 caagaaatgc tgggagtatg aagtaactga tgctgtcgta gaagcggatt cggtgtcaat
     3361 cgattgggat gagggagacg gaacctgcca tttgaacgcc cgcagtaccg accacggctt
     3421 gacatgggaa gctatctggg gcgatccggg gatcaaattg ggatggacga tgaccttgac
     3481 tcagtatgtc gcccgtagcg gggagatcgt cttgtggggc gattggatcg gcgagtcggg
     3541 tagcatcggt aagatgatgt tccagctatc gccaacgcca tagtaatgga ctgccgtgtc
     3601 ttctgatcgc tatcggattc gtcaaactag gacgctaccc gagattcacc aaaccccccc
     3661 cgcctcgacg cccacgcggc gttgcgatac cctccccacc ccaacggccc aacgaaagga
     3721 ccttcgaccg caatgcccga cctgcacctg ctctcccccc gcggcttcac cgccgccggc
     3781 gttaaggctg gcatcaaatc cagcggcaag cgcgacgtcg gcctgctgct gtgcgaggcg
     3841 ctcgccaccg ccgccgccgc gttcaccacc agcaaagtcg tctcccccgc cgtcatcgtc
     3901 ggccaatccc acgtcgcgcg cggccgactc cgcggcgtcg tcgtcaacgc cgggaacgcc
     3961 aacgcctgca ccggtcgtca aggcctgcgc gatgcgcagc gcatgtgcgc gctggcgtcg
     4021 acgatcggca attgcactgc cgagcagatc ctgccgtcgt ccaccggcat catcgggcag
     4081 ctgctgccga tggaaaaagt cgaagcgggg atcacggccg ccggccgcgc gctgggcagt
     4141 tccgccgaac acgcggaggc gttcatggac gcgattctca ccaccgacac caggcgcaaa
     4201 tccgccggcg tcgaattcaa gatcgggcgc gatgtggtgc gacttgccgg cgtctgtaag
     4261 ggcagcggca tgatcggccc tcgcctcggt gtgaacaaag cgaacgctaa gccgcaagcg
     4321 gctcatgcga cgatgctcgc gtatctgacg accgacgccg ccgttgccgc gccgctgttg
     4381 cgcaagctgc tgcagaacgc cgccgacgcc agtttcaacg ccgtcacggt ggacgatcac
     4441 gccagcacca acgacaccgc ctgcctgctc gccagcggca ccagcggcgt caaaatcgcc
     4501 tcccccgcgg cgatcaaaaa attcgccgcc gcactggatg aggtgagcca gtcgctcgcg
     4561 taccagatcg ccgccgatgg tgaaggtgcg acgaaggtgg tgaagatcgt cgtcaccggc
     4621 gcgaccacga cggccgccgc ccgcgccatc gcgcggacga tcgccaattc gccgctggtg
     4681 aagtgcgcga tgaacggcaa cgacccgaat tggggaagaa tcgtctccgc cgccggctac
     4741 gcgggcgcgg cgttcgacgc gaataaaagt tcgttgaaac tacaaggcac caccgttttc
     4801 aaatccggcc agcccgtccc ctttgacgcc gcgaaagtca gcgaagcgtt gaagttgccc
     4861 gaagtcgtcg ccgaattg
//
LOCUS       DATIMH010000479         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2710533, whole genome shotgun sequence.
ACCESSION   DATIMH010000479 DATIMH010000000
VERSION     DATIMH010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2710533"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1587)
                     /locus_tag="VLI90_08355"
     CDS             complement(199..1587)
                     /locus_tag="VLI90_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14257.1"
                     /translation="MKRWLAITALLIVVLCFAWLWWNHVANRQLGQAKDAVRARHEPI
                     DPEDFNPAPLPPERNAAALLQSAASAVASNVDSPSMSNYTFNDAPPYTPLWYALANTA
                     VAADGRALALARQARAIDSADWGWRFHTPVALAFFGAFNSQRNLANLLGDAALHAHVH
                     GDDAEAIERVLDELHQSEIVSNGQGLLIGRLVGIGVEAIAVERIEVLAPGLHISGAAF
                     NPPPATMPTMAVRREVIVALIKQLLDDRSEHEGIRHAILGERMAVLDLIRDSGTHSYI
                     LGPLFNLEIAHVITAFDEDIRAALQTNLPASIKAPSTPPGFLPSRERSRLTRIMSTAL
                     EPAFNRYVLSERRVLSDRFCAAISLAAQLYRVDHGALPADLAALVPAYLPAVPRNPVL
                     DDGSLMEYIALKGTPPGAADRPMITFVSDTPAPAAANAPATPQYGWTAGNRQWRDVTR
                     WEPPAPATAPAE"
     gene            complement(1696..1926)
                     /locus_tag="VLI90_08360"
     CDS             complement(1696..1926)
                     /locus_tag="VLI90_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14258.1"
                     /translation="MTTFTDSEAQRQLSTLLDQARAQGEVRIKRADGQEFIVRPAPRS
                     GLDVGYVDVQPPVTAEEIVAAVREGRERESNP"
     gene            complement(2012..2302)
                     /locus_tag="VLI90_08365"
     CDS             complement(2012..2302)
                     /locus_tag="VLI90_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSV14259.1"
                     /translation="MAVVIDTSVVIACGINEPHRAALLAITVDEELLSPASLPWEVGN
                     ALSSLFKRRRLTVDQALAALDFYQRMSVKLREIDLGAAVRAADRFHIYAYDA"
     gene            complement(2323..>3162)
                     /gene="lipA"
                     /locus_tag="VLI90_08370"
     CDS             complement(2323..>3162)
                     /gene="lipA"
                     /locus_tag="VLI90_08370"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HSV14260.1"
                     /translation="WLKMKMPAGDGYHKLLTLVNDQKLHTVCQSAKCPNMGECWAAGT
                     ATLMILGDVCTRSCGFCHIATGRPPTLDLDEPVRVGFAVKQMNLKHVVITSVNRDELP
                     DGGAAVWAHTIREIRRQSPGTSVEVLIPDFCGDWESLQVVLDERPDILNHNIESVPRL
                     YKVVRPQAKYPRSLKLLQIAREQGLISKTGMMLGLGEEAHEIDAVLDDLVAIGCEILT
                     LGQYLQPTPNHLKVERWVHPDEFASWKVRGEAKGLRHVESGPLVRSSYHAEKQVHAHA
                     AAT"
BASE COUNT          552 a         1068 c          980 g          562 t
ORIGIN      
        1 gggcagcaga ggaacgatga cacgcgcaaa cggctcggca gtcgcttgcg gtttcgcaga
       61 cggacaggcc gcggttcccg gtctgcgaaa ccgcaagcga cgctcgcacg gttcgcgcaa
      121 ataacggttc aattacttct gggcaagatc cttcgactcc gccgagtacg cctgcgctgg
      181 aggatgacat tttcgctact actcggccgg ggctgtcgcc ggcgcgggtg gttcccaccg
      241 cgtgacgtcg cgccactgcc ggtttcccgc cgtccaccca tactgcggcg tcgccggcgc
      301 gttcgccgcc gccggcgctg gtgtgtcgga aacgaacgtg atcatcggtc gatccgccgc
      361 gcccggcggt gtgcctttca gcgcgatgta ttccatcaac gacccatcat cgagcaccgg
      421 attccgcggc accgccggga gatacgccgg caccagcgcc gccaaatccg ccggcaacgc
      481 gccgtgatcc acgcgataca gctgcgccgc cagcgagatc gccgcgcaga accggtcgga
      541 caacacgcgc cgctccgaca ggacgtatcg gttgaacgcc ggttctagcg cggtggacat
      601 gattcgcgta agccgcgacc tttcccgcga tggcaggaac cccggcggcg tggacggcgc
      661 cttgatcgat gccggcaagt tcgtttggag ggcggcgcgg atgtcctcgt cgaacgccgt
      721 gatcacgtgc gcgatctcca gattgaacaa cgggccaagg atgtacgagt gtgtgccgga
      781 gtcgcgaatc aaatcaagga cggccatgcg ctcgccgagg atcgcatgtc gaatgccttc
      841 gtgttcgctg cgatcatcga gcagttgctt gatcagcgcg acgatcacct cgcgccgcac
      901 ggccattgtc ggcatcgtcg ccggcggagg attgaacgcc gcccccgaga tgtgcagccc
      961 cggcgccagc acctcgatgc gctcgaccgc tatcgcttca acgccgatgc cgacgagccg
     1021 accgatcagc aggccctggc cgttgctgac gatctccgat tggtgcagct catcgagcac
     1081 gcgctcgatc gcctcggcgt catcgccatg cacgtgcgcg tgcagcgccg catcgccgag
     1141 caggttggcg agattccgct ggctgttgaa cgccccgaag aacgccaggg ccaccggggt
     1201 gtgaaaccgc cagccccagt ccgccgagtc gatcgcccgc gcctgccggg ccagcgccag
     1261 cgcccgcccg tccgcggcaa cggcggtgtt ggccagcgcg taccacaacg gtgtgtatgg
     1321 cggggcatcg ttgaaggtgt agttggacat cgacggcgaa tcgacgttgc tcgccaccgc
     1381 cgacgccgcc gactgcagca acgccgccgc gttccgctcc ggcggcagcg gggccggatt
     1441 gaaatcctcc ggatcgatcg gctcatgccg ggcccgcacc gcgtccttcg cctgcccgag
     1501 ctgtcgattg gcgacgtgat tccaccaaag ccaggcgaag caaagcacga cgatcaacaa
     1561 cgccgtgatg gcgagccacc gcttcaacca acgaaaccgc ggcggcgttg ggatggaggg
     1621 ttcgacgatt acggaggagg cggcggtcat ttattaccgc cgaagatagc acggatgccc
     1681 ggcgcgcgct gcagattacg gattcgactc tcgttcgcga ccttcccgca ccgctgcgac
     1741 gatttcttcg gcagtcacgg gcggttgcac gtccacatat cctacatcca atccggaccg
     1801 aggcgctggg cgcacgataa actcctgccc gtccgcgcgc ttgatgcgca cttccccctg
     1861 cgcgcgagcc tgatcgagca gagtcgagag ctgacgttgg gcttcactat ccgtaaacgt
     1921 ggtcatgggt tgacctcgcg caatgaaacg ccggcggcgc tggcggctgc cttcagtccc
     1981 cgatccagcg ttaacaactc ggttccggcc tctacgcgtc gtaagcataa atgtgaaagc
     2041 ggtcggccgc tcgaactgct gcccccaagt cgatctcgcg aagcttgacg gacatccgct
     2101 ggtaaaaatc gagggcagcg agagcttgat cgacagtgag gcggcgccgc ttgaacagcg
     2161 acgacagcgc atttcccact tcccacggca gcgacgccgg cgacaacagt tcttcgtcca
     2221 ccgtaatcgc caacagcgcc gctcgatgag gttcgtttat cccacacgcg atgacgacac
     2281 tcgtatcaat cacgaccgcc atgcggacgt cctcattaaa ggtcacgtcg ccgcggcgtg
     2341 ggcgtggact tgcttctcgg cgtggtaact gctgcgcacc agcggaccgc tttcgacgtg
     2401 ccgcaggccc ttggcttcgc cgcggacttt ccagctggcg aattcatcgg ggtgcaccca
     2461 gcgctccacc ttcagatggt tgggcgtcgg ttgcaggtat tggccgagcg tgaggatctc
     2521 gcagccgatc gccaccagat catccagcac cgcgtcgatc tcgtgcgctt cttcgccgag
     2581 ccccagcatc atgccggtct tgctgatcag gccttgttcc ctggcgatct gcagcagctt
     2641 caggctgcgc ggatatttgg cctgcgggcg gacgaccttg tacagccgcg ggaccgattc
     2701 gatgttgtgg ttgagaatgt ccggccgctc gtcaagcacg acctgcagcg actcccagtc
     2761 gccgcagaaa tcggggatga gcacttcgac gctcgtcccc ggcgactgcc ggcgaatctc
     2821 gcggatcgtg tgcgcccaca ccgcggcccc gccatcgggc agctcgtcgc ggttcacgct
     2881 ggtgatgacg acgtgcttca ggttcatctg cttgacagcg aagcccacgc gcaccggttc
     2941 atccaaatcc agcgtcggcg gccggcctgt cgcgatgtgg caaaacccgc agctccgcgt
     3001 gcagacgtcg ccgaggatca tcaacgtcgc cgtgccggcc gcccagcatt cgcccatgtt
     3061 ggggcacttg gcgctttggc agaccgtgtg cagcttctgg tcgttcacca gcgtcagcag
     3121 tttgtgatac ccatcccccg cgggcatctt catcttgagc ca
//
LOCUS       DATIMH010000480         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_693331, whole genome shotgun sequence.
ACCESSION   DATIMH010000480 DATIMH010000000
VERSION     DATIMH010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693331"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2225
                     /locus_tag="VLI90_08375"
     CDS             <1..2225
                     /locus_tag="VLI90_08375"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HSV14261.1"
                     /translation="QVAGNVILDGALNLNLNGLAPANGQQFTLINNIGVNPVVGQFIG
                     LPQNEIVTIGTHAFVVSYTGGDGNDVVLTSTVSPVISIASPGPVVEGNTGQTTNMVFT
                     VSLSDAIPVDVSVDFATSGVTATSGVDFTPESGTLVIPAGQVTGIITVPIIGDLNPEP
                     NETFRVTLSNAVHGNLKAPPFATGTILDDDGPPVVSFGESNVTVNENSGVATITVIRT
                     NPSVAVTVDYATTPGTATPGVDYTAVSGTLSMGIGVVFATFDVPIIDAKITDGSTRDF
                     TVTLSNPHGDNATLGVAAAVVTINDTDTPPPAVSIADAIATEPISGLISETFTISLDK
                     PSDTPVTVQFTTTGGTATEGVDYVATSGTVTIPAGQLSAQATVPIKTDFTQEASETYS
                     VVLSNLNGDAAFGRAIATGTIQNQDVQVVALDATHPFRFVNSSGNLTVIALRGPGSGE
                     VDFLGQTINDAKQVVLNDTTAQSALIVRTSRLQTFLVNITVNGSIGVIDGPTLNLQSS
                     LTSTGGVGRLRLNTIAGGDAISIGSADGDGITGLTAALGNVTNATFTSALPLRMLRVG
                     SWTDSGAGVDITAPSAAVVAAFGNFQADMAIAGDLGTFRVIGALDNADIRVGGSINAL
                     VAVTMSNSTVFAGVQDGVTTVPTSASQFSNNTSTIKLAAVASPAAGAFRNTSIAAPII
                     GTVLINGVATQNGGNPFGIVGNSIKLTRGHDDRIINVTNASLTANPFTDADLLIKLLT
                     "
     gene            complement(2831..>3250)
                     /locus_tag="VLI90_08380"
     CDS             complement(2831..>3250)
                     /locus_tag="VLI90_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV14262.1"
                     /translation="QQVRHLIQDGLGDPALSATGIADKMNYNRGSLSRMFHRHAGLTI
                     MECITQMRLQQAEFLLQQTDDRIGDIARKCGFRDLSYFTRWVKKHVGSVPHKLRDSTA
                     WIEAKAQHKPAAVAARAATFSPLPEAGVSLTSRFPRR"
BASE COUNT          606 a         1041 c         1040 g          563 t
ORIGIN      
        1 tgcaggtggc tggcaacgtc attttggacg gagcgctcaa cctcaacctg aacggattgg
       61 cgccggcgaa tggccagcaa ttcacgctca ttaataacat cggcgttaac ccggtcgtcg
      121 gccaattcat cggtctgccg caaaacgaaa tcgtaaccat cggcacgcac gcgttcgtcg
      181 tcagctacac cggtggcgac gggaacgacg tggtgctgac gtcaaccgtc tcgccggtca
      241 tcagcatcgc ttcgccgggg ccggtcgtcg aaggcaacac gggccagacg accaacatgg
      301 tcttcaccgt gagcttgtcg gatgcgattc cggtggacgt cagcgtggac ttcgcgacca
      361 gcggtgtgac cgccacgtcc ggcgtggact tcacacccga aagcggcacg ctggtcatcc
      421 ccgcggggca agtgaccggc atcatcaccg tgccgatcat tggcgatctg aaccccgagc
      481 cgaacgagac gttccgcgtc acgctgtcca acgcggtgca tgggaacctg aaggcgccgc
      541 cgtttgccac cgggacgatc ctcgacgacg acggcccgcc ggtcgtctcc ttcggtgaat
      601 cgaatgtcac cgtgaacgag aactccggcg tggcgacgat cacggtcatc cgcaccaatc
      661 cgtcggtggc ggtcaccgtc gattacgcta ccacccccgg cacggccacg ccgggcgtgg
      721 attacaccgc cgtctccggc acgctgagca tgggcatcgg cgtcgtcttc gcgaccttcg
      781 acgtgccaat catcgacgcg aagatcaccg acggcagcac gcgcgacttc accgtcacgc
      841 tgtccaaccc acacggcgac aacgcgacgc tgggcgttgc ggccgcggtc gtgacgatca
      901 acgacaccga cacgccgccc ccggcggtgt cgatcgccga cgccatcgcc accgaaccga
      961 tctcggggtt gatcagcgag acctttacga tttcgctcga caagccctcg gacacgccgg
     1021 tgaccgtgca gttcacgacg accggcggga cggccaccga aggtgtcgat tacgtggcaa
     1081 cgtcgggaac ggttacgatt cccgccggcc agctgagcgc acaggccacg gtgccgatca
     1141 agaccgactt cacccaggaa gcctccgaaa cgtacagtgt ggtgctgagc aacctgaacg
     1201 gcgacgccgc cttcgggcgg gccatcgcga cgggcacgat tcagaaccag gacgttcagg
     1261 tggtcgcgct ggatgcgacg catccgttcc gcttcgtcaa ttccagcggc aacctgaccg
     1321 tcatcgcgct gcgcgggccg ggcagcggtg aagtcgattt cctcgggcag acgatcaacg
     1381 acgccaagca agtggtgttg aacgacacga ccgcgcagtc ggcgttgatc gtccgcacca
     1441 gccggttgca gacgttcctg gtgaacatca ccgtgaacgg ttcgatcggc gtgatcgacg
     1501 ggccgaccct gaacctgcag agcagcctga catccaccgg cggcgttggc cggctgcggt
     1561 tgaacaccat cgccggtggg gacgcgatca gcattggttc cgccgacggt gatggcatca
     1621 ccggcctgac ggctgcgctg ggcaatgtca ccaacgcgac gttcacgtcg gccctgccgc
     1681 tgcgcatgct gcgggtgggc agctggaccg acagtggcgc gggcgtggac atcaccgcgc
     1741 cctcggcggc ggtcgtcgcg gcgttcggaa acttccaggc cgacatggcg atcgccggtg
     1801 acctgggcac cttccgcgtc atcggcgcgc tcgacaacgc tgacatccgc gtcggtggca
     1861 gcatcaacgc gctggtggcg gtgacgatga gcaacagcac cgtcttcgcc ggtgtgcagg
     1921 acggcgtgac gaccgtgccg accagcgcat cgcagttcag caacaacacc tcgacgatca
     1981 agctggccgc cgtcgccagc cccgccgccg gggcgttccg caatacgagc atcgccgccc
     2041 cgatcattgg cacggtgctg atcaacggcg tcgccacgca gaacggcggc aacccgttcg
     2101 gcatcgtggg caattcgatc aagctgaccc gcggtcacga cgaccgaatc atcaacgtga
     2161 ccaacgcgag cctgacggcc aacccgttca cggatgcgga cctgctgatc aagctgctga
     2221 cgtaagggcg gacaggcgtc gcaaaaaaga gatcgaacgc aacaccgggc ttgccgcttc
     2281 ctgagcggca agctcttttt tgggttctgt gatggcggga gtggtcaatg cggccgcgac
     2341 gacgcgaagg cgcgaagaca ggcccgaagg ttaggagcgt gtcgcgctgt gagcgcggca
     2401 cgtacgcgtg cgcagtcgaa gattaaatcc gattggccat ggagacgctg aaaagggtgg
     2461 ccccctcacg ctgggttcgg aggcctgtcc tgagcgaagt cgaaggatac tccggcgacc
     2521 cggatgcttt tcgatccaca agagatccgg gtcgtcgcga ggacgctccg aacccggcgt
     2581 gggatgcatt tcttcagcga cggtcgtcgc gaaatcaatc gttcctgccg cattcggaaa
     2641 aacttggtag cgcttaatta gatccttcga ctccggcgag tacgcctgcg ctggaggatg
     2701 acattgcgcg cggggtgcaa cactcgctca aacttttgcg cgacgacaaa gctcgaggct
     2761 ttgccccggg acggttttcg acgttcggtc ccacggcata gccgtgggct tttttgcgct
     2821 tttttagaac tcaccggcgc gggaagcgcg atgttaagga cacgcccgct tcgggtaggg
     2881 ggctgaacgt tgccgcgcgc gccgccaccg ccgcgggttt gtgttgggct ttggcttcga
     2941 tccaagcggt cgaatcgcgc agcttgtgcg gcacgctacc gacgtgtttt ttgacccagc
     3001 gggtgaagta ggacaggtcg cggaacccgc acttgcgggc gatgtcgccg atgcggtcat
     3061 ccgtctgctg cagcaggaac tcggcctgtt gcaggcgcat ctgcgtgatg cattccatga
     3121 tggtcagccc cgcgtggcgg tggaacatcc ggctgagcga cccgcggttg tagttcatct
     3181 tgtcagcgat gccggtggcg gacagggctg gatcacctaa gccgtcctgg atcagatgcc
     3241 gcacctgctg
//
LOCUS       DATIMH010000481         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_403264, whole genome shotgun sequence.
ACCESSION   DATIMH010000481 DATIMH010000000
VERSION     DATIMH010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403264"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..799
                     /locus_tag="VLI90_08385"
     CDS             62..799
                     /locus_tag="VLI90_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HSV14263.1"
                     /translation="MLTPLTTILPLLADVIEDAGPGPGAQSANDALLQWPSHWPLQGD
                     LLAWCHSTGPGVATLLIIGGIVYLLFGYYIFKALVTLNSAILGAYFGAVLGDKSGTAV
                     PAAIVGALLAAAATWPVMKYAVAVMGGLFGALLGATIWRLCDLDPHFAWSGAMTGLVF
                     FGLLSFLLFRQCIMTYTSLQGSVMLVFGILALIFKYDSIANTINHSFELKPFLLPLSI
                     FIPTLLGFIYQQAVFPAAAPASGPPKK"
     gene            complement(811..1761)
                     /locus_tag="VLI90_08390"
     CDS             complement(811..1761)
                     /locus_tag="VLI90_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14264.1"
                     /translation="MLTISLSLRSWSALACGLLASALIAPAHATVTFAQLDDFQDGTT
                     MGWQQGFQATNPPANVATGGPAGAGDRYLQNISTGGFGASSRQVMFNNAQWTGDFFHA
                     GVTRINAMLANFGSTTLSMRVAMQGGTNFSEYSSTNAISLPADGAWHAVTFEMDPTTM
                     TLVAGTDPLSDVLSSVIQFRMLSSSSGPTYTGADPIASTLGVDNVRALTLPGDATLDG
                     KVDVADLGVLATNYSLTGDWSKGDFNFDGNVNVGDLGALATNYNTSIPTGASVAAAEA
                     SVAVPEPGGMVVLISAGVLWSIRRLRLRNPSGVVCQAVSG"
     gene            1945..>2436
                     /locus_tag="VLI90_08395"
     CDS             1945..>2436
                     /locus_tag="VLI90_08395"
                     /inference="COORDINATES: protein motif:HMM:NF016494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HSV14265.1"
                     /translation="MHSKTLRELATEIGADLVGDGDVVVSSVNTLQDARPGQLSFLAN
                     PKYEKDLESTQASGVIVAANSKFDRVPTLRAKDPQLTYQRAVLALHGPRRHPHMGVHP
                     QAFVDATASVGEGTVIYPGAYVGPQVRIGRDCVLFAGVIVYERCAIGDRVVIHGGSVI
                     GADG"
BASE COUNT          422 a          756 c          794 g          464 t
ORIGIN      
        1 attttagttc tgcattctgc attcatcatt ctgcatttcc gaagaccgat tggagtaaac
       61 cgtgttgact cccctgacca ccatcctccc actgctggct gacgtcattg aagacgccgg
      121 tcctggaccc ggcgcgcaga gcgccaacga tgcgctgctg caatggccga gccattggcc
      181 gctgcagggc gacctgctgg cgtggtgcca cagcaccggt cccggcgtgg ccacgctgct
      241 gatcatcggg gggatcgtct acctgctgtt cggttactac atcttcaaag cgctggtgac
      301 gttgaactcc gcaatcctgg gcgcgtactt cggcgccgtg ttgggcgaca agagcggcac
      361 cgcggtgccc gcggcgatcg tcggcgcgct cctcgccgcc gcggcgacct ggccggtgat
      421 gaagtacgcc gtggccgtga tgggcgggct gttcggcgcg ctgctgggcg cgacgatctg
      481 gcggctgtgc gatctcgatc cccacttcgc ctggagcggc gcgatgacgg gcctggtctt
      541 cttcggcctg ctgtcgttcc tgctgtttcg ccaatgcatc atgacctaca ccagcctgca
      601 gggctcggtc atgctcgtct tcggcatcct cgcgctgatc ttcaaatacg actcgatcgc
      661 caacacgatc aaccacagct ttgaattgaa accgttcctg ctgccgctgt cgatcttcat
      721 cccgacgctg ctggggttca tctatcaaca agccgtcttc cccgcggccg cgccggcgtc
      781 gggaccacca aagaagtagt gcgcgcccct tcagccgctt acggcttggc agaccacgcc
      841 gcttggattg cgaagccgca agcggcggat actccagagc actccagcgg atatcaagac
      901 gaccatcccg cccggctctg gtacagcgac gctcgcttcg gccgcggcga ctgacgcacc
      961 agtcggaatg ctcgtgttgt agttcgtcgc cagcgcgccc aggtcgccga cgttcacgtt
     1021 gccgtcgaag ttgaagtcgc ctttgctcca atccccggtg aggctgtagt tcgtcgcgag
     1081 cacacccagg tcggcgacgt ccactttgcc gtcgagcgtt gcgtcgccgg ggagcgtcag
     1141 ggcacggacg ttgtctacgc cgagcgtcga ggcgatcgga tcggcgccgg tgtaagtcgg
     1201 gccgctggaa gacgagagca tgcggaattg aatgacgctg ctcagcacat cgctcagcgg
     1261 gtcggtgccg gccaccagtg tcatcgtcgt cgggtccatt tcgaacgtca ccgcatgcca
     1321 agcgccgtcg gcgggcaggc tgatcgcatt cgtgcttgag tattcagaaa agttagtccc
     1381 accctgcatg gcgacgcgca tgctgagcgt cgtcgagccg aagttcgcca gcatcgcatt
     1441 gatgcgcgtg acgccggcat ggaagaagtc gccggtccac tgggcgttgt tgaacatcac
     1501 ctgccgggag ctggcgccga acccaccggt cgaaatgttt tgaaggtaac gatcgcccgc
     1561 gccggcgggg ccgccggtcg caacgttggc cggcgggttt gtcgcttgga agccttgctg
     1621 ccaacccatc gtcgttccgt cttggaaatc atcgagctga gcgaacgtga cggtggcgtg
     1681 cgccggggcg atgagcgcgc tcgcgagcag gccacaagcc agcgcagacc aggagcggag
     1741 cgagagggaa atcgtcagca cagcgcgcct ccggatatga ctcgaaaccc aatccagcgg
     1801 agcgattgtg cctgtcttgg ggatgcccag caaaaagggt gcaaaggatg cacggtggaa
     1861 gcggcgggcg cgaacgagtg ggagtgaacc gggtcgcgcg cggcccgtgt agcccggcgc
     1921 tgttaaagtc cgtagaatcg ccgtgtgcac tcaaaaacgc tgcgggaact tgcgaccgaa
     1981 atcggcgccg acctggtcgg cgacggcgat gtcgtcgttt cgtccgtcaa cacgctacag
     2041 gacgcccggc cggggcagct gagctttttg gcgaatccca aatacgaaaa agacctggaa
     2101 tcgacgcagg cgtcgggcgt catcgtcgcg gcgaattcga agtttgaccg cgtgccgacg
     2161 cttcgtgcaa aagatccaca attaacctat cagcgagcgg tgctggcgtt gcacggcccg
     2221 cggcggcatc cgcacatggg cgtgcatccg caggcgtttg tcgatgcgac cgccagcgtc
     2281 ggtgaaggca cggtgattta tcccggcgcg tatgtggggc cgcaggtgcg catcggtcgc
     2341 gactgcgtgc tgtttgccgg cgtcatcgtg tatgagcggt gcgcgatcgg cgatcgcgtc
     2401 gtgatccacg gcggcagcgt gatcggcgct gatgga
//
LOCUS       DATIMH010000482         7545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3289684, whole genome shotgun sequence.
ACCESSION   DATIMH010000482 DATIMH010000000
VERSION     DATIMH010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3289684"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..373)
                     /locus_tag="VLI90_08400"
     CDS             complement(86..373)
                     /locus_tag="VLI90_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HSV14266.1"
                     /translation="MPGMKPVELPVAKQTTLEQTCPLSGLAVGSRGRVVCVCGEIDAR
                     RRLLEMGFCNGAMVETIRRAPFGDPVEYRLRGYCLSLREQQACCVTVTPDD"
     gene            636..1538
                     /locus_tag="VLI90_08405"
     CDS             636..1538
                     /locus_tag="VLI90_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSV14267.1"
                     /translation="MRILITHDGSECSSAALADLPNAGFPGTATVLTFGVAEPPVGDP
                     QVEAYLMNRRMTELRGSVTRAADRVRSMFPGWRVDTEVWPGVAGAAIAERAASFHPEL
                     IVVGSHDRGRFGRLLLGSVSQSVLHHANCSVRVARRPLQRYEEGLRLLIGLDGSHDAD
                     AVVRAVAARYWPVSAEVRVVGVVDAGVPLAVAEPALSAVGDGATLSSISAYYEEHSDP
                     LEAMAESLKTAAAALRAAGVRAAPQVLEGDPAETLLYEAEQWGADCIFVGARGLGRLE
                     RLLLGSVSSTVATGARCSVEVVRT"
     gene            complement(1881..4652)
                     /locus_tag="VLI90_08410"
     CDS             complement(1881..4652)
                     /locus_tag="VLI90_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HSV14268.1"
                     /translation="MVPAAEIIAPKSNQPHVGLDAATPRQSLDAIFHPRTVAVIGATE
                     TVGSVGRTVLWNLISNPFGGTVFPVNPKRSNVMGIKAYPSIADVPAAVDLGVIVTPAA
                     TVPEVIAQCVDAQVRGAIVISAGFRELGPDGAALEQRVLEQARRGGMRIIGPNCLGVM
                     NPIVGLNATFAGGMARPGNVAFISQSGALLTAILDWSFREQVGFSSFVSTGSMLDVSW
                     GDLIDYLGNDPRTHSILIYMESIGDARAFLSAAREVALTKPIIVIKPGRTEQAAKAAA
                     SHTGSLTGGDEVIDAAFRRCGVLRVNNIADLFYMAEVLSKQPRPRGPRLSIVTNAGGP
                     AVLATDALITGGGQLAPVSPGTMDQLNKLLPAPWSHNNPIDVLGDAGAERYAKTLEIA
                     AKDPDSDGLLVILTPQDMTEPAQTAELLRQYAKIDSPFPKPVLASWMGGPDVQAGEEI
                     LNRAGIPTFPYPDTADRMFTYMWRYTYNLRGLYETPAMPDDRTVGAPDRQVADRLIND
                     ARAAGRTLLTEHESKALLKSYGIPTVTTEIATTVEDAVRAAEHIGYPVVLKLHSNTIT
                     HKTDVGGVKLNLGDAAAVKTAFEDIRRGVTKVAGEKAFDGVTVQLMVRLDEAYELIVG
                     ASPDPQFGPVLLFGTGGQLVEVFKDRGLALPPLNATLARRMMEQTKIYRALQGVRGRK
                     PVDLAALEHLMVRFSQLVVEQRWIKEIDINPLIASSTQLLALDARVVLHDSATPENRL
                     PKPAIRPYPVQYVRPWKMGDGTAVTIRPIRPEDEPLLVQFHQTLSDQSVQQRYFAPLK
                     LDQRTSHERLTRVCFNDYDRELALVVDRQDPASKRRQIIAVGRLSKTPHLNEAEVAVL
                     ISDVAQGRGLGTELVQMLVAAARDERVGRLVAYILPENQSMLRICRKLDFRIEQTPGD
                     TMLTAVRDL"
     gene            complement(4709..5284)
                     /locus_tag="VLI90_08415"
     CDS             complement(4709..5284)
                     /locus_tag="VLI90_08415"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HSV14269.1"
                     /translation="MFAEQWSKLVSASVAPVVVISASGLLCLAFYNRLAAIVSRLRTV
                     QRERLQEQLALRAALERRDAISVRRHRRMLNNLKHQTNGIIGRAHIVRLTLLGLLATV
                     AFLVVSSMLNGLALLWPPLAFAAATLFVAGMLCLLSGVIAAMIELKMSLDVVTLESKL
                     VSELTDPAAHVVDERGDEVAALNGNDIRDHG"
     gene            complement(5415..6368)
                     /locus_tag="VLI90_08420"
     CDS             complement(5415..6368)
                     /locus_tag="VLI90_08420"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HSV14270.1"
                     /translation="MKTTKKPIARAKRTTSSPSAGAAPVNSRKVAPAAAAAEPIVHVL
                     SDSTGNLPRHILTALLTQFPPHSISARFENFVRTAHQVDRVLELAAKHGGIVCHAIVS
                     PELKQRVADHCEAAAIPSCDLTGGIVDFLTSTTGVQPRCDTDALHRLDEAYRRRIGAM
                     EFTLSHDDGLGLDTLHEADVVIVGVSRTSKTPTSILLAQQGYQTANISLAMGVEPPQQ
                     MLAIPKNKIIGLVIDPAQLSMIRTRRQAAWNMTPTTYGDPNNVAAEVAWSRALFQRHH
                     WPTLDVTDQAVEETAARVVEMLGLSALPSGRPTTGGGLLQD"
     gene            complement(6387..7094)
                     /gene="hypB"
                     /locus_tag="VLI90_08425"
     CDS             complement(6387..7094)
                     /gene="hypB"
                     /locus_tag="VLI90_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HSV14271.1"
                     /translation="MSIDTTIPLSERFSANARIAAENRATFALDGVTAISLIGPPGSG
                     KTSLVESLARHVAGRIRMSVIVGGLAAARDAERLARIGVLAVPVQSDNIAATHVHSAL
                     PELSLRRTDLVVIEGAGNTLSPVEFDLGQDLRVAVFSVAGGDDKAAAYPRLVAESDLV
                     LLTKADLLPYVNFDLELFTSDIQRLNPTLEVIATSARTGEGLQQWEQWLMRHWSPSKA
                     HRASGRLTDPASDWLVE"
     gene            complement(7158..>7545)
                     /locus_tag="VLI90_08430"
     CDS             complement(7158..>7545)
                     /locus_tag="VLI90_08430"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HSV14272.1"
                     /translation="DYLMHWSRMRRPMRDCPRIFHVNWFRKNEHGQYVWPGYGDNIRI
                     LRWIIDRCHGRAYAVETPLGWMPRAEDIDLGGLDVLRSDIESVQSIDLEAFKAEILGH
                     QELFLKLSGDLPKEIIFQRELLISRL"
BASE COUNT         1299 a         2360 c         2476 g         1410 t
ORIGIN      
        1 gtgaagcacg taatcgtggg cagtcgaagg atcttggcga gtgacgccgc ggacaggtga
       61 gaacgcgctg tagcacaccc tggcatcaat catccggcgt caccgtcacg cagcacgcct
      121 gctgctcgcg aagcgacagg caatatccac gcaggcggta ttcgacggga tccccaaacg
      181 gcgcacgccg gatggtttcg accatcgcac cattgcagaa ccccatctcc agcaaccgcc
      241 ggcgggcatc gatctcgccg cacacgcaga ccacgcggcc gcgggaaccg acggctagtc
      301 cgctcagggg acaggtttgt tccaaagtcg tctgtttcgc gacgggtagt tcaacgggct
      361 tcatgccagg cattgagcaa ccgtcattcc gcgaagagtt ccaagaattg tgcgtatttc
      421 agctaggaaa tgagcctcta gatggttcag ctcccgcgac tgagaaacga tcgccgctgc
      481 cggttccagc tgtggcactg atgggagttt gagtaatcat cggggcaatc ccctatggct
      541 tttgccgatg actgtccgat cgactcggca cgcgcgttcg gatgacaatt gaaataagga
      601 cgtcacccat gcgacgcgaa acgaggcaaa gcgcaatgcg aatcctgatc acccacgacg
      661 gctccgaatg ctccagcgcg gcactggccg atctgcccaa tgccgggttc cccggcacag
      721 ccaccgtttt gaccttcggc gtggccgagc caccggtggg cgatccgcag gtcgaggcgt
      781 atctgatgaa ccgccgcatg acggagcttc gtggttcggt gacgcgcgcc gccgatcgcg
      841 tgcgcagcat gttcccgggt tggcgcgtgg acaccgaggt ttggcccggc gtggccgggg
      901 cggccatcgc cgagcgggcg gcgtcgttcc atccggagct gatcgtcgtc ggctcgcacg
      961 accgcgggcg gttcggccgg ttgctgctgg gcagcgtctc gcaatcggtg ctgcatcacg
     1021 ccaactgctc ggtgcgcgtc gcccgccggc cgctgcagcg ttacgaggag gggctgcgat
     1081 tattgattgg cctggatggt tcccacgacg ccgatgccgt cgttcgcgcg gttgccgcgc
     1141 gctattggcc ggtgtcggcc gaggtccgcg tcgtcggtgt agtcgatgcc ggcgttcccc
     1201 tggcggtggc cgagccggcg ctgtcggcgg tgggcgatgg cgcgacgctg tcgtcgatct
     1261 cggcatatta cgaagaacat tccgatcctt tggaggcgat ggccgaatcg ctcaagaccg
     1321 ccgccgccgc gttgcgagcg gctggcgtgc gagcggcgcc gcaagtgctg gaaggagatc
     1381 ctgctgagac gctgctgtac gaagcggagc agtggggggc ggactgcatc tttgttggag
     1441 cgcgcgggct gggccggctg gaacggctgc tgttgggcag cgtctcgtcc accgtcgcca
     1501 cgggcgcgcg atgctcggtc gaggttgtgc gaacgtaaga gcgtgcgccc aagtcacgca
     1561 atcgtcatcc tgagcgcagc acgtactcgt gcgcagtcga aggatttggc cgaaaagtcg
     1621 gtgaacgatt gctttgcaag tggcgcagca gtcgcttgcg gtttcgcaga ccagtgactg
     1681 tggtttgtcc gtctgcgaaa cggcaagcga ccgccgcgcc atccccgcaa ctacaggttc
     1741 atctacttct cggcacgatc cttcgactcc ggcgagtacg cctgcgctca ggatgactgg
     1801 agccagagac gctgcgacgc ctgcgtccct ttcttgtgaa aggcgacgca ggcgtcgcag
     1861 ctacagagtc tgagttgtgc ttacaagtcg cgcaccgccg tcagcatcgt gtcgccgggg
     1921 gtttgctcga tgcggaagtc gagcttccgg cagatccgaa gcatcgactg gttttccggc
     1981 agaatgtacg ccaccaaccg cccgacgcgt tcgtcacggg cggcggccac cagcatttga
     2041 accaactcgg tgccgagccc gcggccctgg gcgacgtcgc tgatcagcac cgcgacctcc
     2101 gcctcgttca gatgcggcgt tttacttaac cgtccgacag cgatgatttg ccggcgcttg
     2161 ctggccgggt cttggcggtc caccaccagc gccaattcgc ggtcgtaatc gttgaagcat
     2221 acgcgcgtca gccgctcgtg cgacgttcgc tgatccagtt tcagcggggc gaagtatcgc
     2281 tgttgaaccg attgatcgct gagcgtctgg tggaactgaa ccagcagcgg ctcgtcctcc
     2341 ggtcgaatcg gccggatcgt caccgcggtg ccgtcgccca tcttccacgg ccgcacgtat
     2401 tgcacggggt acggacgaat cgcgggcttc ggcagccggt tttccggcgt ggccgaatca
     2461 tgcagcacca cgcgggcgtc caacgccagc agctgagttg acgatgcgat cagcgggttg
     2521 atgtcgatct ctttgatcca gcgctgctcg acgaccagct gactgaatcg aaccatcaga
     2581 tgttcgagcg cggccagatc gacgggcttt cggccgcgca cgccttgcag cgcgcgatag
     2641 atcttcgtct gttccatcat ccgccgggcc aacgtcgcgt tcagcggcgg tagcgcgagg
     2701 ccgcggtcct tgaagacttc aaccagctga ccaccggtgc cgaacagcag gaccggaccg
     2761 aattgtggat cgggcgaggc gccgacgatc aactcatacg cctcgtccag ccgaaccatc
     2821 agctgcaccg tcacgccatc gaacgccttt tccccggcca ccttcgttac gccgcggcgg
     2881 atgtcttcga atgcggtctt caccgcggcg gcgtcgccca ggttcagctt cacgccgccc
     2941 acgtcagtct tgtgcgtgat cgtgttgctg tgcagcttca ggacgacggg atagccaatg
     3001 tgctcggcag ctcgaaccgc gtcttcaacc gtggttgcga tctcggtcgt gacggtgggg
     3061 atgccgtaag acttgagcag cgccttggac tcgtgctcgg tcaatagcgt gcgaccggcg
     3121 gcgcgggcgt cgttgatcag ccggtccgcc acctgtcgat cgggcgcgcc gaccgtgcga
     3181 tcgtccggca tcgccggggt ctcgtacaag ccgcgcaggt tgtacgtata gcgccacatg
     3241 taggtgaaca tgcggtcggc ggtgtcgggg taggggaatg tcggaatgcc ggcgcgattg
     3301 agaatttctt cgccggcctg cacatcgggg ccgcccatcc agctggcgag caccggcttg
     3361 gggaatgggc tgtcgatctt tgcatattgc cgcagcaatt cggcggtttg cgccggctcg
     3421 gtcatgtcct gcggggtgag gatgacgagc agaccatcgc tgtccggatc tttcgcggcg
     3481 atctccagcg tcttggcgta acgctccgcg ccggcgtcgc cgagcacgtc gatcggattg
     3541 ttgtgactcc acggggccgg cagcagcttg ttcagttgat ccatcgtgcc gggtgacacg
     3601 ggcgcgagtt gcccgccgcc ggtgatcagc gcatcggtcg ccagcaccgc ggggccgccg
     3661 gcattggtga cgatgctgag ccgcggcccg cgcggacgcg gttgcttgga caacacctcg
     3721 gccatgtaga acaggtcggc gatgttgttc acgcggagca caccgcagcg gcgaaaggcg
     3781 gcgtcgatca cttcgtcccc gccggtcagg gatccagtgt gcgaagcagc cgccttcgcc
     3841 gcctgctcgg tgcggccggg tttaattacg atgattggct tcgtcagtgc gacttcgcgc
     3901 gccgcagaaa gaaacgcgcg ggcgtctccg atgctctcca tgtagatcag gatcgaatgc
     3961 gtgcgcggat cgttgccgag gtagtcgatc agatcacccc aggagacgtc gagcatcgat
     4021 ccggtcgaga cgaacgacga aaagccgact tgttcgcgga agctccagtc gagaatggcg
     4081 gtgagcagcg cgccactttg gctgatgaac gcgacgttgc ccggccgcgc catgccgccg
     4141 gcgaaggtgg cgttcagccc gacgatgggg ttcatcacgc ccaggcaatt ggggccgatg
     4201 atgcgcatcc cgccgcggcg ggcctgttcg agcacgcgct gttccagcgc ggcgccatcg
     4261 gggcccaatt cgcgaaaacc ggcggagatc acgatcgcgc cgcgcacctg tgcgtcaacg
     4321 cactgcgcga tgacttccgg cacggtcgcc gccggcgtga cgatgacgcc caggtccacc
     4381 gcggcgggga catcggcgat cgacgggtat gccttgatgc ccatgacgtt cgaccgcttc
     4441 ggattgaccg gaaagaccgt gcccccgaag gggttgctga tcaggttcca cagcacggtg
     4501 cggccgacgc tgccgacggt ttcggtggcg ccgatgacgg ccacggtgcg cgggtggaag
     4561 atcgcatcca gcgactgccg cggcgtcgcc gcgtcgagcc cgacgtgcgg ttgatttgat
     4621 ttcggtgcga tgatttcggc tgcggggacc atttgagaag ctcctgatgt tgaaaaagat
     4681 attgcgtttc ggcttgcttg cgcgcgcgtc atccatgatc gcgaatgtca ttgccgttca
     4741 gcgccgccac ctcgtcaccg cgttcatcga cgacgtgggc ggccgggtcg gtcagttcgg
     4801 aaaccagctt gctttccagc gtcacgacat cgagcgacat cttcagctcg atcatcgcgg
     4861 cgatgacgcc cgacaacagg cacagcatgc cggcgacgaa cagcgtcgcg gcggcaaatg
     4921 ccagcggtgg ccagagcagg gccaatccgt tcaacattga cgagacgacg aggaacgcga
     4981 cggtcgccag caggccaagc agcgtaaggc gaacgatgtg cgcgcgaccg atgatgccgt
     5041 tggtctgatg cttgaggttg ttcagcatgc gccgatgccg gcgaaccgag atcgcgtcgc
     5101 gacgctcgag cgcagcgcga agggctagct gttcctgcag ccgttctcgc tgcacggtgc
     5161 gcaggcgcga gacgatcgct gccaggcgat tgtagaacgc aagacaaagc agcccgctgg
     5221 cggagatcac gacgacgggc gcgaccgagg cgctgacgag tttagaccat tgttctgcga
     5281 acatactgtg atcacattcc tttcaactgc atcatctgcc gcgtagcggt cgccccgcag
     5341 gggcacgtcc tttccgtgcg aggggaaaaa cacgtgcccc tgcggggcta ccgctacgcg
     5401 gggaacgcgc ggctttagtc ttgcagcaga cctccaccag tcgttggccg gccagatggc
     5461 agggctgaca accccagcat ctccaccacg cgggcggcgg tttcttcaac cgcctggtcc
     5521 gtaacgtcca gcgttggcca gtggtggcgt tggaacaatg cccggctcca agcgacttcg
     5581 gcggcgacgt tgttgggatc accataggtc gtgggcgtca tgttccatgc ggcttgccgc
     5641 cgcgtgcgga tcatcgacaa ctgcgcgggg tcgatcacca gcccgatgat tttgtttttc
     5701 ggtatcgcca gcatctgctg cgggggttca acgcccatcg ccagcgagat gtttgctgtc
     5761 tgatatccct gctgggcgag caggatgctc gtcggcgtct tgctcgtgcg cgatacgccg
     5821 acgatcacga cgtccgcttc atgaagcgta tcgagtccca ggccgtcgtc gtggctcagc
     5881 gtgaactcca tcgcgccgat ccggcggcga tacgcttcat ccagccgatg cagtgcgtcg
     5941 gtgtcacatc gcggctgcac gccggtggtg ctggtgagaa aatcgacgat cccgccggtt
     6001 aaatcacagc tggggatagc cgccgcctcg cagtgatcgg cgacgcgctg tttcagctcg
     6061 ggcgagacga tcgcgtgaca cacgatgccg ccatgcttcg cggctagctc gagcacgcgg
     6121 tccacctggt gtgccgtgcg gacgaagttt tcgaatcgcg cgctgatcga gtgtggcggg
     6181 aactgcgtca gcaatgccgt caggatgtgc cgcgggaggt tgccggttga atccgacagc
     6241 acgtggacga tcggctcggc tgcagccgcg gcaggcgcga ctttgcggga attgactggc
     6301 gcagcgccag cgctgggact ggacgtcgtg cgcttcgcgc gcgcgatggg cttcttcgtc
     6361 gttttcatgg gcggtccgat cctacgtcac tccactaacc aatcgctcgc cgggtccgtc
     6421 agccgaccgc tcgcgcggtg ggctttgctc ggagaccagt gtcgcatcag ccactgttcc
     6481 cattgctgca gaccttcacc cgtgcgagcg gaggtagcga tcacctccaa cgtcggattc
     6541 aggcgctgga tgtccgacgt gaacagctcg aggtcgaagt tcacgtacgg caacaggtcg
     6601 gccttggtca gcaacacgag gtccgattca gcgacgagcc gcggatacgc ggcggctttg
     6661 tcatcaccgc cggcgacgct gaagacggcc acgcgcaggt cctgacccag gtcaaattcc
     6721 acgggcgaga gcgtattgcc tgcgccttcg atcaccacca gatctgtccg ccgcaggctt
     6781 agctccggta gcgccgaatg cacgtgcgtc gccgcgatgt tgtcgctctg caccggcacg
     6841 gccagcacgc cgatgcgcgc caatcgttcg gcatcgcgcg ccgcggccag gccgccgacg
     6901 atcaccgaca tccgaattcg tcccgccacg tgccgcgcca gggattcaac cagcgaggtc
     6961 ttaccggaac ccggcgggcc gatcaggctg atggcggtca cgccatccaa cgcaaaggtg
     7021 gcacggttct ccgccgcaat tcgggcgttg gcggagaagc gttcgctcag cgggatagtt
     7081 gtgtcgatgc tcatgacaat cgcgtgcctc gcccacgatc acgcgcgggc gaggggatca
     7141 aagggcgaga tcacgcgtta cagccggctg atcaacagct cgcgctggaa gatgatctcc
     7201 ttgggtaaat ccccggacaa cttcaggaac agctcctgat gtccaaggat ttctgccttg
     7261 aatgcttcaa ggtcgatgct ctgcaccgat tcgatatccg accgcagcac gtccagcccg
     7321 cccaggtcga tgtcttcagc gcgcggcatc cagcccagtg gcgtctccac ggcgtatgct
     7381 cggccgtggc agcgatcgat gatccagcgc aggatgcgga tgttgtcgcc atagcccggc
     7441 cagacgtatt gcccgtgctc gttcttgcgg aaccagttga catggaagat gcgcgggcag
     7501 tcgcgcatcg ggcggcgcat gcgggaccag tgcatgaggt agtcg
//
LOCUS       DATIMH010000483         4792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2422598, whole genome shotgun sequence.
ACCESSION   DATIMH010000483 DATIMH010000000
VERSION     DATIMH010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2422598"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VLI90_08435"
     CDS             <1..228
                     /locus_tag="VLI90_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14273.1"
                     /translation="LAPRCSLLLAHRPDDEVLPADCSRSVYRSAREPKRLLLKERVRH
                     GLDEWADALPGIIREWIRSELGKPVGEVTKA"
     gene            complement(215..1528)
                     /locus_tag="VLI90_08440"
     CDS             complement(215..1528)
                     /locus_tag="VLI90_08440"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HSV14274.1"
                     /translation="MKTSVDAPLAKANHLAKIEPMPRPQYSSLQDWIAREAISFTVDS
                     PVELDAAVDRMIASLGDAIELLGLGEAMHGGAESLAFRNRLFQRLVAVHGYSAIAVES
                     SFPRGHIANEYVAGRGPASYDAVAEAGFSHGFGRMDGNRELLAWMRQYNADSAHPVKL
                     SFYGFDGPMEMMGTDSPRQLLVFALDYLASIDAAAVAQRRERIESLLGTDADWENPAA
                     MMDASKSIGQSPAAVALRLATEDLIAEMSIRRPELIARSDAHRYGEAVHHAMVARQLL
                     NYHAAVAGNSPTRLADLLGMRDALMADNLAYVVARERDRGKVLAFAHNSHLKRGQAHW
                     QLGSHSITWWPAGAHLGEMLAKRYVIIGEAVGVSEGNGIASPEAGALEVHLLAAPGPA
                     RFVPTYAARGLADEMPMRSASAKNSTYFPLAPQAVSDFDALLMLS"
     gene            1580..2158
                     /locus_tag="VLI90_08445"
     CDS             1580..2158
                     /locus_tag="VLI90_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HSV14275.1"
                     /translation="MKRQSDQPGLFDTGSVQLPEGFKYQPDLITVNEERELVRQIAPL
                     PFADFEFQGFTGKRRVVSFGWRYDFNERELQKVDDMPPFLMSLRERAARFAQLPPDGL
                     QHVLVTAYGPGAAIGWHRDKAVFAEVVGISLLAPCPFRLRRKFADKWQRMTIAVEPRS
                     AYLLAGPSRTEWEHSIPPMDRLRYSITFRNFR"
     gene            complement(2136..2885)
                     /locus_tag="VLI90_08450"
     CDS             complement(2136..2885)
                     /locus_tag="VLI90_08450"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HSV14276.1"
                     /translation="MPESTASITPIPADQHIQISAIIFPRMDQIDLTGPFAVLSRLPN
                     SSVQLVWKDTAPLRDFRGLGFVPDATFADAKPADLLLVPGGPGQEDLMADQVALSFIA
                     AQAKRAKCIFSICTGALVCGAAGLLNGRFATTHWRSVHLLKYFGATTVDQRVVVDGKV
                     VCAAGLTAGIDGALTVAAHLRGEAAAKAIQLEIQYAPEPPFDCGSPASAPAEMVARSR
                     NATAALTAQRLATAKRIAAKLQIAVSENSGT"
     gene            complement(3057..4247)
                     /locus_tag="VLI90_08455"
     CDS             complement(3057..4247)
                     /locus_tag="VLI90_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV14277.1"
                     /translation="MVLRRNLIILLAVACGVAVANLYYNQPLLENIGRSFGTDPAGTG
                     LIATFSQAGYAAGMLAFVPLIDLIERRRLIVTLQVLVTVALLAVAMAPTFNTLLAASF
                     AVGCLTVAPQLIIPYTASVAEPKQQGRMIGSLYTGLLLGILLARTVSGFVGQQWGWRT
                     MYFAAAAAAALLALLLRLALPKSSPGHDHSYAQLMASLATMVRSYPALRESAIIGGAL
                     FGSFSAFWTTLAFRLAQPPYHFGPRGAGLFGIIGAVGAIGAPIAGRLADRHGPRLVIG
                     FAIAVTLAAFAVFLGFDLHIAGLIAGVIVLDLGVQAAQVCNQSRIFALAPGAQGRANT
                     VYMIVYFTGGALGSFLAATAWQHWHWPGVCGVGLGLVLFAAAAHARGSLPTIAPEPAN
                     AAAQ"
     gene            4356..>4792
                     /locus_tag="VLI90_08460"
     CDS             4356..>4792
                     /locus_tag="VLI90_08460"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HSV14278.1"
                     /translation="MIRMLIAASIPVITGLSTVFAAAPTSAPAAALRQAVLPGFQADP
                     SARVFGDRLYIYASHDLAGNRTWDNMLDFHAFVSDDARTFEDLGQIFSVDDLTWAKQK
                     LWAPDCAQRDGKYYLYFAADAQIGVAVADSPRGPFKDALGKPLL"
BASE COUNT          883 a         1520 c         1565 g          824 t
ORIGIN      
        1 cttgcgccgc ggtgttcgct gctgctggcc caccggccgg acgatgaggt tcttcccgct
       61 gattgttccc ggtcggtcta tcgatcggcg cgcgagccga agcgtctgct gctcaaggag
      121 cgcgttcgcc acggactgga cgaatgggcg gacgcgttgc ccggcatcat tcgcgagtgg
      181 atccgcagcg aattgggcaa accggtgggt gaagttacga aagcatgagc agcgcgtcga
      241 aatcagacac ggcctgtggc gccagaggaa agtaggtcga attcttggcg ctggctgacc
      301 gcatcggcat ttcatctgcc agtccccgtg ctgcatacgt cggaacaaat cgcgccggtc
      361 ccggcgcggc caagaggtgc acttcaagcg cgccggcctc cggcgaggcg atgccgttcc
      421 cttcggatac gccgaccgct tcaccaatga tcacatagcg cttggcgagc atttcaccca
      481 gatgcgcgcc ggccggccac caggtgatcg aatgcgatcc gagctgccaa tgggcctgac
      541 cgcgcttgag gtggctgttg tgggcgaacg ccagcacttt tccgcgatct cgctcgcgcg
      601 cgaccacgta cgcgaggttg tctgccatta gcgcgtcgcg catgccgagc aagtcggcca
      661 ggcgagtggg cgaattcccc gccaccgccg cgtgatagtt caacagctgc cgggcgacca
      721 tcgcgtgatg gacggcttcg ccgtagcggt gcgcgtcact cctggcgatc agctccggcc
      781 gtcgaatgct catctctgcg atcaaatctt ccgtcgccag ccgcagcgcc acggccgcgg
      841 gtgattgacc gatcgacttg ctcgcatcca tcatcgccgc agggttctcc cagtcggcgt
      901 cagttcccag caatgactcg atccgctccc ggcgctgggc gacagcggcg gcgtcgatcg
      961 acgcgagata atcgagcgcg aacaccagca gttgccgcgg gctgtcggtg cccatcattt
     1021 ccattgggcc gtcgaagcca tagaagctca gcttcaccgg atgcgcggaa tcggcgttgt
     1081 actgccgcat ccacgcgagc aattcgcggt tgccgtccat gcggccaaag ccgtggctga
     1141 agcccgcctc cgccaccgcg tcgtacgagg ccggcccgcg accggcgaca tattcattgg
     1201 cgatgtgccc gcggggaaaa ctgctttcga ccgcgatggc gctgtaccca tgcaccgcga
     1261 caaggcgctg gaacaaccgg ttacgaaacg cgagcgattc agcgccaccg tgcatcgcct
     1321 cgcccaggcc gagcagttcg atcgcatcgc cgagggatgc gatcattcga tcgacggcag
     1381 cgtccaattc aacgggcgag tcaacggtga aggagattgc ctcgcgcgcg atccagtcct
     1441 gcagcgatga atattgcggc cgaggcatcg gctcgatctt cgcaagatga tttgctttgg
     1501 caagcggggc atcgaccgat gttttcatcg cctcctgaat cgccgtgaca gtggtacaaa
     1561 gtctacgggc gaggcatcga tgaaacgaca gtcagaccaa cccggcttgt tcgacacggg
     1621 cagcgttcag ctgcccgaag ggttcaagta tcagccggat ctgatcacgg tgaacgagga
     1681 gcgggaactg gtccggcaga tcgcgccgct gccctttgca gattttgaat tccaaggctt
     1741 caccggcaaa cgccgcgtcg tatcgtttgg ttggcggtac gatttcaatg agcgcgagct
     1801 acagaaggtg gacgacatgc cgccgtttct gatgtcgctg cgcgagcgtg cggcccgatt
     1861 tgcccagttg ccgccggatg gcctgcagca cgtgctcgtc acggcgtatg ggccaggagc
     1921 cgccatcggt tggcaccgcg acaaagcggt cttcgcggag gtcgtcggca tttcgctgct
     1981 cgccccctgc ccattccgcc ttcggcgtaa gtttgcggac aaatggcagc ggatgacgat
     2041 cgcagtcgag ccgcgctcgg cgtatctgct ggcgggcccg tcgcgcacgg agtgggaaca
     2101 cagcatcccg ccgatggacc ggctcaggta ctcgatcacg ttccggaatt ttcgctgact
     2161 gcgatctgca gctttgccgc gatgcgcttg gcggtggcta aacgctgcgc agttagcgcc
     2221 gcagtggcgt tgcgcgatct ggcgaccatc tcggcgggcg cacttgcggg cgagccgcaa
     2281 tcgaacggcg gctcgggcgc gtattgaatc tccagttgaa ttgccttcgc ggcggcctcg
     2341 ccgcgcaggt gcgcggcgac cgtgagcgcg ccatcgatcc ccgcggtgag cccagcggcg
     2401 cagacaactt tgccatccac gacgacgcgc tggtccaccg tggtggcgcc gaaatatttc
     2461 agcaggtgga ccgaacgcca atgggtcgtc gcgaatcgcc cgttgagcaa accggcggcg
     2521 ccgcaaacga gcgcgccggt acagatggag aagatacact tcgcccgctt cgcctgcgcc
     2581 gcgatgaacg acagggcgac ctggtctgcc atcaggtctt cctggcccgg cccacccggc
     2641 acaagcagca gatcggccgg cttggcatcg gcgaacgttg cgtccggcac gaaaccgagc
     2701 ccgcggaaat cgcgcagcgg tgcggtgtcc ttccacacca gttggactga ggagttgggc
     2761 agccgggaca gcacggcgaa cggacccgtc aggtcgatct ggtccatccg tgggaagatg
     2821 atggcgctga tctggatgtg ctggtccgcc gggatgggcg tgatcgacgc ggttgattct
     2881 ggcattgcac cgtctccgtc taaagcagct tcagaaccat caacgtgaca cgggcctccg
     2941 gcccgtgcat ggtgctttgg tagtagtaga gcgtcgtctt ttgaatttca ctgcatcagg
     3001 catgggccgg aggcctgtgt caccgtaaag gctctccacg tactccaaac cacttcttac
     3061 tgcgctgccg cgttcgcggg ttcgggggcg atcgttggca acgacccgcg ggcgtgagcg
     3121 gcggcggcaa acagcaccag gccaagtccc acgccgcaca cgccgggcca atgccaatgt
     3181 tgccacgccg tggcggcaag gaacgagccg agcgcaccac cggtgaaata gacgatcatg
     3241 tataccgtat tggcccggcc ctgcgcgccg ggggcgagcg cgaagatgcg gctctggttg
     3301 cagacctggg ccgcctgcac gcccaggtcc aggacgatca cgccggcgat cagcccggcg
     3361 atgtgcaaat cgaaaccgag aaacacggcg aacgccgcga gcgtcacggc gatcgcgaag
     3421 ccgatcacga gccgcgggcc gtggcgatcc gccaatcgtc cggcgatcgg cgcgccaatc
     3481 gcgcccaccg cgccgatgat gccgaacaat cccgccccgc gcgggccaaa atgataaggc
     3541 ggctgtgcca gacgaaacgc gagcgtcgtc cagaacgcgc tgaacgatcc gaacagcgcg
     3601 ccgccgatga tggctgattc gcggagcgct ggataagagc gcaccatcgt cgccagcgac
     3661 gccatgagct gagcgtacga atggtcatga cccggcgatg acttgggtaa tgccaggcgc
     3721 aatagcaaag caagcaacgc ggcggcggcc gcggcggcga aatacatcgt ccgccagccc
     3781 cactgctgac cgacaaaacc ggaaaccgtg cgcgccagca aaatgcccag cagcagcccg
     3841 gtgtagagcg aaccgatcat gcgaccttgc tgcttcggct cggccacgct ggcggtgtat
     3901 gggatgatca gttggggcgc caccgtcaaa catccgacgg cgaaactggc ggcgagcagc
     3961 gtgttaaatg tcggcgccat cgcgaccgcg agcagcgcca ccgtgaccag aacttgcagc
     4021 gtcacgatca accgccggcg ctcgatcagg tcgatcagcg gcacgaatgc gagcatgccc
     4081 gcggcatatc cggcttgaga gaacgtcgcg atcaatccgg tgccggcggg gtcggtgccg
     4141 aacgatcggc cgatgttctc cagcagcggc tggttgtagt aaaggttcgc gacggcgacg
     4201 ccgcaggcga cggcgagcag gatgatgagg ttgcggcgga gcaccatggg ccatcgacaa
     4261 taggcgcgcg ctgcgagcgc gtcgcgttgc gcgcgttgca tggcgtgcat cgtagcgcgc
     4321 gtcaggttat ggttggcgac gccgggaggt tcacgatgat tcgcatgctg atcgccgctt
     4381 cgatccccgt catcacaggt ctatctactg tcttcgccgc ggcgccgacc agcgcgccgg
     4441 cagctgcgtt gcggcaagcc gttctgccgg ggtttcaggc ggatccctcg gcccgcgtgt
     4501 tcggcgatcg gctttacatc tacgcgtcgc acgacctggc ggggaaccga acgtgggaca
     4561 acatgctcga cttccacgcg ttcgtgtccg atgacgcgcg gacgttcgaa gacctggggc
     4621 agatcttcag cgtggatgat ctgacctggg ccaagcagaa gctgtgggcg ccggattgcg
     4681 cccagcgcga cggcaaatac tatctatact ttgcagcgga tgcgcagatc ggcgtggccg
     4741 tcgccgattc accgcgcggg ccgttcaagg atgcgctggg caaaccgctg ct
//
LOCUS       DATIMH010000484         5416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1558506, whole genome shotgun sequence.
ACCESSION   DATIMH010000484 DATIMH010000000
VERSION     DATIMH010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558506"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VLI90_08465"
     CDS             complement(<1..1147)
                     /locus_tag="VLI90_08465"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HSV14279.1"
                     /translation="MTTVPATPPPVRTEGLTMSFGGVTVLKNASITLHTGEVHGLVGE
                     NGAGKSTLAKMIGGVYVPKAGTIFVNGTPTSLPTPRAAIHQGIALIHQEPLTFPDLTV
                     AENIFIGHQPRAGGKVDWAAMVRRSGVILASLGVRIDARAKVRGLSIADQQMVEMAAA
                     LSQQARVFLMDETTAALTPNEVKDLFRIMRQLKDGGAALAFIGHRMEEIFEICDRITI
                     LRDGEIVGDRLTRQTSIPEVLRLMVGRPMEAMFAKEVKHSLGEPVLEVKNLSRAGEFT
                     NINFQVRAGEIVGLAGLVGAGRTEVSRAIFGVAPPDGGEVLIQNKPVKITGPRDGMRH
                     GLALVPEDRQHDGVLMPMSVWQNCTLAVADRMSRLGWLRDTAGKRATQ"
     gene            1413..2288
                     /gene="lpxA"
                     /locus_tag="VLI90_08470"
     CDS             1413..2288
                     /gene="lpxA"
                     /locus_tag="VLI90_08470"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HSV14280.1"
                     /translation="MPRIHPTAIVDPLALIGDDVSIGAYSIIKGPVRIGDGTVVHEHS
                     HLHGKTTIGQSCQIGPAAYVGLEPQHLHADPNIGQLIVGDHVIIRETATIHRSTTAGD
                     DHATRIGDHCFLMGGVHIAHDCVLNERVIAANSVIMGGHCQVGAAAFLGGGAAFHQFV
                     RIGRLAIISGNEPMTQDMPPFGAQKNGFLKGYNAVGCKRAELSNTAIRGIRAAFRILH
                     SHRVVSAALVEMEAEAGDVPEVREIVAFIRASKRGIVPSHSPTSRSHSDEDDGAVNGE
                     GARDRERPVRAAALR"
     gene            2302..3327
                     /locus_tag="VLI90_08475"
     CDS             2302..3327
                     /locus_tag="VLI90_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HSV14281.1"
                     /translation="MPASTTDRRFLVADIGGTNIRIASFTTDPRQREAEITYRLNPAT
                     NKPYQVIESLRDYRAKMSGAFAAACLGVAGRVKGAEVQITNRPDLIRRDEVAAVLEVE
                     PSRVLLVNDMPPHLASVDRLQPSEVIEIKPGESDPRGNRAILMPGTGVGVGGAVWVPA
                     IDVHRPFPSEGGHVDFAPRDQQQDALMRFLRPIAKAIGHTHASDEIAFAGEGIRRIYT
                     FLSGGSPDDVAKAPKSEEITQAVATNNLPADDLRARTIELYLKILGAAAGNLALMFTA
                     TGGVYLGGSICLTLRHLLATPPFIDAFKTSGPEALRPLMAEVPVRLIDYKDSGLLGAG
                     VLALRLV"
     gene            complement(3445..4566)
                     /locus_tag="VLI90_08480"
     CDS             complement(3445..4566)
                     /locus_tag="VLI90_08480"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14282.1"
                     /translation="MTIDSTNPVHIADPINARILAVSEDKIQGFQADPMSAIAQASGV
                     DIVTVRDRIAAMLRAGTIRRVRQTLLATNLAHGALVAWQVPEEKLSAAFDWMFQQDPF
                     SGHVVIRSTDAAIAGSRYRLWTTVKVPQGYSLAKHCDFLAKQTGAISYQLLPAKCLFA
                     LGVGHVRRRGMEPGSKSEALAEVINTNIVELNELEWRVLVALKREFTPEEILREDLWQ
                     PRADEAGVPLSTFYDAALELNARGVIGRFSTFLEHVKPLKTGEQVTRYNALFHWAVPP
                     GREIDAGREIGRFHIMTHAYWREAGSEFGNVNIMGVAHGTDKELVLAHKRAIDEHLKS
                     VGIEVSYTNVFWGGRSEIKPSEISPFAYEAFCREFRIER"
     gene            4779..>5416
                     /locus_tag="VLI90_08485"
     CDS             4779..>5416
                     /locus_tag="VLI90_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14283.1"
                     /translation="MSSPQDPTPTESTPPPPEIAPATATTAPTEPGIPPEDSLVIMLP
                     DESSQPKDLGLWAGVLVILTLVAYWPSTDGSFLWNDDRHVSDNRLLAQPGGLSTIWFQ
                     RWQDEKAYPLPEYHPVAYTSYWLDYHLFGHDAQTRMPTPFMYRLSNLLLHALTAVLVW
                     LTLRRLKVPGAWVAAAAFALHPLNTEAIAYISQRATVLAGALFFGAIYTYLM"
BASE COUNT          934 a         1762 c         1717 g         1003 t
ORIGIN      
        1 cttgtgtcgc gcgtttgcca gcggtgtcgc gcaaccagcc gagccggctc atgcgatccg
       61 ccaccgccag cgtgcaattc tgccacacgc tcatcggcat cagcacgccg tcgtgttgtc
      121 ggtcttcggg aacgagtgcc agcccgtgcc gcataccgtc gcgcgggcca gtgatcttca
      181 cgggtttgtt ctgaatcaag acctcgccac catcaggcgg ggcgacgccg aagatcgcgc
      241 gcgagacttc ggttcggccc gcgccgacca acccagccag gccgacgatc tcgccagcgc
      301 gcacctggaa gttgatgttc gtaaactcgc cggcgcgcga caggttcttc acctccagca
      361 ccggctcgcc cagcgaatgc ttcacttcct tggcgaacat cgcttccatc ggccggccga
      421 ccatcaggcg cagcacttcg gggatgctcg tctggcgcgt gaggcgatcg ccgacgattt
      481 cgccgtcgcg caggatcgtg atgcggtcgc agatctcgaa gatctcttcc atgcgatggc
      541 cgatgaacgc cagcgcggcg ccgccgtcct tcagctgccg catgatgcgg aagaggtctt
      601 tgacctcgtt cggcgtcagc gccgccgtcg tctcgtccat cagaaagacg cgcgcctgct
      661 gcgacaacgc ggcagccatc tcgaccatct gctgatcggc gatgctgagc ccgcgcacct
      721 tcgcgcgggc gtcgatccgc acgccgagcg acgccagaat cacaccgctg cggcgaacca
      781 tcgcggccca gtccaccttg ccgccggcgc gcggttgatg gccgatgaag atgttctccg
      841 cgacggtcag gtcagggaac gtcaacggct cctggtgaat cagcgcaatg ccctgatgaa
      901 tcgccgcccg cggtgtcggc agggatgtcg gtgtgccgtt gacgaaaatt gtgccagcct
      961 tggggacgta aacgccgccg atcatcttcg ccagcgtcga ttttccagcg ccgttttcac
     1021 cgaccagccc gtgcacttcg ccggtgtgca gggtgatgct ggcgttcttt aagacggtca
     1081 cgccgccgaa ggacatggtg agtccttcgg tgcgcaccgg cggcggggtg gcggggacgg
     1141 tcgtcacggg cgatgtacat atcatgccac gcgggcggaa gtaagagccg cgtgtccggt
     1201 gtgacaattg tgcaagcgag atcacgatcc actcacgccg ggttcggagg tactccgaag
     1261 aggtactccg acgacccgga ttctggcgcg cgcgaagaat ctcgcttcgt cggagtacct
     1321 ccgaaccggg cgtgggtcag gtttctcgaa tggttgccat tttcagtcag agccagaatt
     1381 gatcacctcg cttacgcgga taaaatcccg ccatgcctcg gattcacccc acggccatcg
     1441 tcgatccgct ggcgctgatt ggtgacgatg tttccatcgg cgcgtacagc atcatcaagg
     1501 gccccgtgcg catcggcgat ggcacggtcg tccacgaaca ctcgcacctg catggcaaga
     1561 ccacgatcgg ccaatcgtgc cagatcgggc cggccgcgta tgtcgggttg gagccgcagc
     1621 acctgcacgc ggacccgaac atcggccagc tgatcgtcgg cgatcatgtc atcattcgtg
     1681 agaccgccac catccaccgc tcgaccacgg cgggggacga tcatgcgacg cgcatcggcg
     1741 atcactgctt cctcatgggc ggcgtgcata tcgcgcacga ttgcgtgttg aacgaacgcg
     1801 tgatcgccgc caacagcgtg atcatgggcg ggcattgcca ggtcggcgcg gccgcgttcc
     1861 tcggtggtgg ggcggcgttc catcagttcg tgcgcatcgg gcgcttggcg atcatctctg
     1921 gcaatgaacc gatgacccag gacatgccgc cgttcggcgc gcagaagaat gggtttctca
     1981 agggctacaa cgcggtgggt tgcaagcgcg cggagttgag caacacggcc atccgcggca
     2041 ttcgcgcggc gtttcggatc ctgcacagcc accgcgtcgt ctcggcggcg ctggtggaaa
     2101 tggaagccga ggccggcgat gtgccggagg tgcgcgagat cgtcgcgttc atccgcgcgt
     2161 ccaagcgcgg catcgtgccg tcgcacagtc caacgtcgcg atcgcattcg gatgaagacg
     2221 acggcgccgt gaacggcgag ggcgcgcgcg accgcgagcg ccctgtacgc gccgccgcgc
     2281 ttcggtagga ttgcccgcgg catgcccgct tcaaccaccg accgccgctt tctcgtcgcc
     2341 gacatcggcg ggaccaacat tcgcatcgcg tcgttcacca ccgacccgcg gcaacgcgag
     2401 gcggagatca cctatcgcct caatcctgcg acgaacaaac cgtatcaagt gatcgaatcg
     2461 ctgcgcgatt accgcgcgaa gatgagcggc gcgttcgccg ccgcgtgcct gggcgtggcg
     2521 ggtcgcgtga agggcgccga ggtgcagatc accaatcgcc cggacctgat ccgccgcgac
     2581 gaggtggccg ccgtgctgga ggtcgagccg tcgcgggtgc tgctggtgaa cgacatgccg
     2641 ccgcatctgg cgagcgtcga tcgcttgcag ccgagcgagg tgatcgagat aaaaccgggc
     2701 gagagcgatc cgcgcggcaa ccgcgcaatc ctgatgcccg gcaccggtgt gggtgtcggc
     2761 ggcgcggtgt gggtgccggc catcgacgtg catcgcccgt tcccatccga aggcggccac
     2821 gtcgatttcg ccccgcgcga ccagcagcag gatgcgctga tgcgtttcct gcggccgatc
     2881 gcgaaggcga ttggtcacac acacgccagc gacgagatcg ccttcgccgg cgaaggcatt
     2941 cggcgcatct acacgttcct cagcggcgga tcaccggacg atgttgccaa agcgcccaag
     3001 agcgaagaga tcacgcaagc cgtcgcgacg aacaatctgc ccgcggatga tcttcgcgcg
     3061 cgaacgattg agctatacct gaaaatcctg ggcgccgccg cggggaattt ggcgctgatg
     3121 ttcaccgcca ccggcggcgt gtacctgggc ggcagcatct gcttgacgct gcggcacctg
     3181 ctggcgacgc cgccgttcat cgacgcgttc aaaaccagcg gccccgaggc gcttcgcccg
     3241 ctgatggcgg aagtgccggt gcggttgatc gattacaagg acagtggatt gctgggtgcg
     3301 ggggtgctgg cgctgcggtt ggtgtgagaa tcgctcgttt tcattcagcc ataatagccg
     3361 gaggcgccaa gccccggtcg gttcgaacgc gaaaacaccg ggcctaccgg ccccggctat
     3421 gaagatccat tttccgcaat aaagtcagcg ctcgattcgg aactcccggc aaaacgcctc
     3481 atacgcaaac ggcgaaatct cgcttggttt gatctcgctt cggccgcccc agaagacgtt
     3541 ggtgtagctc acctcgatgc cgacgctctt gaggtgttcg tcgatcgcgc gcttgtgggc
     3601 cagcaccagt tctttgtctg ttccatgggc gacgcccatg atgttgacgt tgccgaattc
     3661 gctccccgct tcgcgccagt aggcgtgggt catgatgtgg aagcggccga tctcgcggcc
     3721 ggcgtcgatt tctctgcctg gcggcaccgc ccagtggaac agggcgttgt agcgcgtgac
     3781 ctgctcgccg gtcttcagcg gtttgacgtg ttctaaaaac gtgctgaacc ggccgatgac
     3841 gccgcgggcg ttcagctcca acgcggcgtc gtagaacgtg gacagcggta cgccggcttc
     3901 gtcggcgcgc ggctgccaga ggtcttcgcg cagaatctct tccggcgtga actcgcgctt
     3961 gagcgcaacc agcacgcgcc attcaagctc gttcaactcg acgatgttcg tgttgatcac
     4021 ctcggccagc gcttcgcttt tgctgcccgg ttccatgccg cggcggcgca cgtgaccgac
     4081 gccgagcgcg aacagacact tagccggcag cagttggtat gagatcgcgc cggtttgctt
     4141 cgcgagaaag tcgcaatgct tcgccagcga atatccctgc ggcaccttca ccgtcgtcca
     4201 caggcggtag cgcgaccccg cgatggcggc gtcggtcgag cggatgacga cgtgcccgct
     4261 gaacgggtct tgctgaaaca tccaatcaaa cgcggcggat aacttttctt ccggcacctg
     4321 ccaggcgacg agcgcgccgt gcgccaggtt ggtggccaat aacgtttgac gaacccggcg
     4381 gatggtgccg gcgcgcagca tcgcggcgat ccggtcgcgc acggtgacga tgtcaacgcc
     4441 gctcgcttgc gcgatggcgc tcattggatc tgcctgaaat ccttgaatct tgtcttcgga
     4501 gaccgccagg atgcgggcgt tgatggggtc ggcgatgtga acggggttgg ttgagtctat
     4561 ggtcatcagc atttcagttg acggcgttgt agccgggggc ggaagccctg gtctgttcga
     4621 acgtacaaaa gaccggggct tccgcccccg gctatgacgc gcgggatcag ttggcgcgct
     4681 gccaattgtc gccccatcat tttccattgc aaccgcgttc gtttatcttt cgcggctcgc
     4741 cactttgaaa cccgcgtggc ggaaagatga cgcgccccat gagctcaccg caagatccga
     4801 caccgactga atccacacca ccgccgcctg agatcgcgcc ggcgacggca acgaccgcgc
     4861 ccaccgaacc tggcatcccg ccggaagatt cgctggtcat catgttgccg gatgaatcgt
     4921 cgcagcccaa agaccttggc ctgtgggccg gcgtgctggt catcctgacg ctcgtcgcgt
     4981 attggcccag caccgatggc tcgttccttt ggaacgacga ccgccacgtc tcggacaatc
     5041 gcctgctggc ccaacccggc gggctgagca ccatctggtt ccagcgctgg caggacgaga
     5101 aggcttatcc gcttcccgaa tatcaccccg ttgcctacac cagctattgg ctggactatc
     5161 acctgttcgg ccacgatgcg caaacgcgca tgcccacgcc gttcatgtat cgcctgagca
     5221 acctgctgct gcacgcgctg acggcggtgc tggtctggct cacgcttcgg cgactgaaag
     5281 ttcccggcgc ctgggtcgcc gcagcggcgt tcgcgctgca tccgctcaac accgaagcca
     5341 tcgcctacat cagccagcgc gccaccgtgt tggccggcgc gctgttcttc ggggcgatct
     5401 acacgtactt gatgtt
//
LOCUS       DATIMH010000485         6764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5315482, whole genome shotgun sequence.
ACCESSION   DATIMH010000485 DATIMH010000000
VERSION     DATIMH010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5315482"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..2769)
                     /locus_tag="VLI90_08490"
     CDS             complement(115..2769)
                     /locus_tag="VLI90_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HSV14284.1"
                     /translation="MNEDRAPVTRRMFLGAVGAAAVVVAKSRTGVFAAEAQAAAPFAG
                     ATLLSDGWQMQSSEAVKATGDALSKPDYAPIGWYSVSVPTTVLAGLVASGEYPDIYYA
                     ENLKKVPIARFRVPWWYRKTFTVPNAAAAGTQVWLHFKGINYRANIWLNGQQIADASK
                     AVGAYRDLEFNITAAVAGRVQNVLAVEVTGPKKDRDLSITFVDWAPQPPDLNMGIWQD
                     VELRTSGPVALRNPHVLTDLDVPSLASAKLTVLADLVNATDHPVTGTLEARVDQLRFT
                     QAVQLAPRETKTVELPAQTMSNARIWWPWQLGKPEMHSLALSFTVNGVVSDQSTTNFG
                     VRKVASRLDKGHLLFTVNGVDLLIMGAGYCPDLLQRRVMPDRPSWQEDHVRYVRDMNL
                     NTIRLEGKLEDDAFFDICDRHGMLVMAGWCCCSPWEQWNKWKDEQHAVAEESLRYQIR
                     KLRTHPSLLVWLNGSDNHPPKDVEEKYLAIESELKWPCPTISSATAKKSPASGATGVK
                     MEGPYKWEPPIYWLTDKKKGGAWGFNTEVGPGPVPPPLESLEKMLPPEHRWPIDAMWN
                     YHCGSGKTFDNITDFTDALDARFGKPTNIADFAWKSQAQAYETIRAMYEGFRRNKFTS
                     ATGEIQWMLNNAWPSLIWHLYDYYWRPGGAYFATKTACEPLHVLYSYDDQSIVVANNS
                     LKAFENLAVTTQVYDPQAKLLHEHSGACAVAANATSVAYTLPKLESLPVTYFVRLTLA
                     DASQQVISVNCYFLSTKPDVLAQKASDKDDWNITRCTSYADVTALEKLPKATLTVQQL
                     AVDRSRDEHEARVHVSNTSAAIALMVRLKLTRGDGGEEVLPIRWDDNYFMLLPGEKRE
                     IAARWMPNDLGDAKPTVTVDCFNNARGA"
     gene            3103..4566
                     /locus_tag="VLI90_08495"
     CDS             3103..4566
                     /locus_tag="VLI90_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14285.1"
                     /translation="MPPITPQGSRTGLLTSLVIFVILFVTATIFAIYFNVQLRSKEQD
                     FEHYKLQYKDVVADTAFSTPEFTSLQALKTDTSNPTYADMSILDIALKQRADLAQAIT
                     GTPGDPAAAQKTVTDTLQSVNKKLAPEGVKLPSDHDNLAGAVNLLSDTVASQQKQLQD
                     ANNKLKEAQAAQAAAEKKLADVSAAHDKALADARAQGQAALDQLNQQFTSNKGVIDQL
                     QKDSADAAKKAQDAAQAVQTQMAELNNQVKKEADANRALQAKLGNRRVDVTSAAVRQG
                     DGRIVRIPGNGICYINLGQGDQVTPGMTFEVYDQAEGVPPIPQNATGDEQLPVGKASI
                     EITRVGTTSSECRIVRTSPGAVLSEGDVIANLVYDPNNKYNFFVYGNFDLANTGRPNP
                     ADGEVIKRLVTQWGGKVVDQVNVDTDFVVLGKEPTLPSYSKEELESPLNADKLAKAQA
                     ELEKYHEIMQTARDLHIPILNQNRFLYYIGYYDQAKR"
     gene            complement(4666..>6764)
                     /locus_tag="VLI90_08500"
     CDS             complement(4666..>6764)
                     /locus_tag="VLI90_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HSV14286.1"
                     /translation="NYLPKYEVVILDEAHTVEDVAGQHFGLKISEAGLRYNLRALYDV
                     KKGRGLLSVHGSCANDAIRDIVELNDLSEGFFDRCARWHEQFGRGNGRVRDKEIIEND
                     LTPKLRDLSLHIKAMLTHIEPEEEISELTAAAAKIANFAEVTDSILKQTMPDAVYWMD
                     VNGRTPKRVSLHAAPVNVAEGLKRYLFEKVHSVVMTSATLCASSGSSAPRHGRDARAT
                     QDAAPRSTGVSPVPSEPRDASAISVRQGAHLPHWTRFGAVYAVTFRLGDSLPQNVLDG
                     WVAEREEIAKRAVSAGREMTAVEVDQLRDLHSEKVQKYLDAGYGECLLRDSRAAAIVA
                     GALRKFDGVRYRLLAWCIMPNHVHALVQPVGDFDLAKILHSWKSFTAHAINKALKRSG
                     EVWQTETYDHLVRDEPDLYHCADYAWNNPDEAGLKNWKWRWRIDGNTMADTAVERSTG
                     VSPVSNNARHGRDARATSFAYIQSRLGVEKCKTLALGSPFDYEAQATLYIETDLPEPN
                     DTLRFLPAACDRIIHYLQQTNGGAFVLFTSYKMLIDAANRLNSRLQELGYPLLVQGQN
                     APRKILLDRFRATANAVLFGTASFWQGIDVQGDALRNVIIVKLPFAVPDEPLVEARLE
                     AVQRAGGNPFIEYSVPEAIIKLKQGFGRLIRSKTDKGIVAILDSRVKTKRYGRMFLQS
                     LPPCRVVEVGGAARDA"
BASE COUNT         1224 a         2182 c         2084 g         1274 t
ORIGIN      
        1 gccttctgct ttcttgccat ccgtggtacc tctcgttacg accgttcaga gtagaacgtc
       61 gtgacttgag ataggttcta aagccccgcg gctgcgattt cgattgcgcc tcggtcacgc
      121 gccgcgggcg ttgttgaagc agtccaccgt caccgtcggt ttcgcgtcgc ccaggtcgtt
      181 tggcatccac cgcgccgcaa tttcgcgctt ctcgcccggc aggagcatga agtaattgtc
      241 gtcccagcgg atcggcagga cttcctcgcc gccgtcgccg cgggtgagtt ttagccggac
      301 catcagcgcg atggcggctg aggtgttgct cacgtgcacg cgggcttcgt gctcgtcgcg
      361 cgagcgatcg acggccaact gctgcacggt cagcgttgcc ttcggcaatt tttccagcgc
      421 cgtgacatcg gcgtacgacg tgcagcgcgt gatattccag tcgtccttgt cgctcgcctt
      481 ctgggccagc acgtccggct tggtggacag gaagtagcaa ttgacgctga tcacctgctg
      541 cgatgcgtcg gctagcgtca atcgcacgaa gtatgtcacc ggcagcgact ccagcttcgg
      601 cagcgtgtac gccacgctgg tcgcgttggc ggccaccgcg caggcgccgc tgtgctcgtg
      661 caggagcttg gcttgcggat cgtacacctg cgtcgtcacc gccaggttct cgaacgcctt
      721 cagcgaattg ttggcaacga cgatcgactg gtcgtcatac gaatacaata catgcaacgg
      781 ctcgcaggcg gtcttggtcg cgaagtacgc cccgccggga cgccagtagt agtcgtacag
      841 gtgccagatc agcgatggcc aggcgttgtt cagcatccat tggatttcac cggtggccga
      901 ggtgaacttg ttccggcgga agccttcgta catcgcgcgg atcgtctcgt acgcctgcgc
      961 ctgtgacttc caggcgaagt cggcgatgtt ggtcggcttg ccgaaacggg catccaacgc
     1021 gtcggtgaag tcggtgatgt tgtcgaacgt tttcccgctg ccgcagtgat agttccacat
     1081 ggcgtcaatc ggccagcgat gctcgggcgg cagcatcttc tccagcgatt ccagcggcgg
     1141 cggcaccggg ccggggccga cttcggtgtt aaagccccac gcgccgccct ttttcttgtc
     1201 ggtgagccag tagatcggcg gttcccactt gtacgggcct tccatcttta cgccggtcgc
     1261 gccggatgcc ggcgattttt tcgccgtcgc gctggagatc gtcgggcacg gccatttcag
     1321 ttccgactcg atggccaggt atttttcctc gacgtccttc ggcggatggt tgtcgctgcc
     1381 gttgagccag accagcagag acggatgcgt ccgcagcttg cggatctgat agcgcagcga
     1441 ttcctccgcc accgcgtgct gctcgtcctt ccacttgttc cactgctccc acgggctgca
     1501 gcagcaccac ccggccatca ccaacatgcc atgtctgtcg caaatgtcga agaaggcatc
     1561 atcttccagc ttgccttcca ggcgaatcgt gttcaggttc atgtcgcgga cgtagcgaac
     1621 gtgatcctcc tgccagctag gccgatccgg catcacccgg cgttgcaaca gatcggggca
     1681 atagcccgcg cccatgatca gcaggtcaac gccgttcacg gtgaacagca ggtgaccctt
     1741 gtcgaggcgg gaggcgactt tgcggacgcc gaagttggtg gtcgattgat cggagacgac
     1801 gccgttgacg gtgaagctca gggccagcga atgcatctcc ggcttgccca gttgccatgg
     1861 ccaccagatg cgcgcgttcg acatggtctg cgccggcagt tccaccgtct tcgtctcgcg
     1921 cggcgccagt tgaaccgcct gcgtgaagcg cagttgatcg acccgcgcct cgagcgtgcc
     1981 ggtcaccggg tggtcggtcg cgttcaccag gtcggccagc acggtcaact tcgccgacgc
     2041 cagcgatggc acgtccaggt ccgtcagcac atgcgggttc cgcagcgcga ccggcccgct
     2101 cgtgcgcaac tcgacatcct gccagatccc catgttcaaa tccggcggct gcggagccca
     2161 gtccacgaac gtgatgctca gatcccgatc tttcttcggc cccgtcacct cgaccgccag
     2221 cacgttctgc acgcgccccg cgacggcggc ggtgatgttg aactccaggt ctcgatatgc
     2281 cccgacggcc ttgctcgcat cggcgatctg ctggccgttc agccagatgt tggcgcgata
     2341 gttgatccct ttgaaatgca gccagacctg cgtgcccgcc gcggcggcgt tgggcacggt
     2401 gaaggtcttg cgataccacc acggcacgcg aaatcgcgcg atcggcacct tcttgaggtt
     2461 ctccgcatag tagatgtcag gatactcacc gctggcgacg aggccggcga gcaccgtcgt
     2521 cggcaccgac acgctgtacc aaccaattgg cgcgtaatcc ggcttcgaca gcgcgtcgcc
     2581 ggtcgccttg acggcttcgg acgactgcat ctgccaaccg tccgataata acgtcgctcc
     2641 ggcgaacggc gccgcagctt gagcctctgc tgcgaagacg ccggtgcgcg acttggcgac
     2701 gaccaccgcc gcggcgccga cggctcccag gaacatgcga cgggtgaccg gggcgcgatc
     2761 ttcgttcatc tttgctgacc tcaaaagtga cggaagaaca aaggattgca ctgccggcca
     2821 ccgagcatat cgccaagcga ctttggaaag caaaattgcg ccgatcgcga ggtctcaata
     2881 gccggggccg gaaggcccgg tctgtttctt ccgcaagcgg cgcgtccgcc gacggacaaa
     2941 agaccgggcc ttccggcccc ggctatcaag gagggtcggc ggcgcacatc atgaacgccc
     3001 gcttttcttg accgtatcaa ttgatgtccg gtacattctg ctcccgcgcg cgtgtctcgc
     3061 gcgtgcgtgc catcgtttat ttcccgcgga ggtttcacac ccatgccccc catcacgccc
     3121 caaggttctc gcaccgggtt gctgacgtcg ttggtgatct ttgtcatcct cttcgtcacc
     3181 gccaccatct ttgccattta cttcaacgtg cagctgcgat cgaaggaaca ggacttcgag
     3241 cactacaagc tgcagtacaa ggacgtggtc gccgacaccg ccttcagcac gccggagttc
     3301 accagcctgc aggcgctgaa gaccgacacg agcaacccaa cctacgctga catgtcgatc
     3361 ttggacatcg cgctcaagca gcgcgctgac ctggcgcagg cgatcacggg cacgcccggc
     3421 gatcccgccg cggcgcagaa gaccgtcacc gacaccctgc agtcggtgaa caagaagctc
     3481 gcgccagagg gcgtgaagct gccgtcggac cacgacaacc tcgccggcgc ggtcaacctg
     3541 ctgagcgaca ccgtcgccag tcagcagaag caattgcagg acgcgaacaa caagctgaag
     3601 gaagcccagg ccgcccaggc cgccgccgag aagaagctgg ccgacgtgag cgctgctcac
     3661 gacaaggcgc tggccgacgc ccgcgcgcag gggcaggcgg ctctggatca actgaaccag
     3721 cagttcacgt cgaacaaggg cgtgatcgat cagctgcaga aggacagcgc cgacgccgcc
     3781 aagaaagcgc aagacgccgc ccaggccgtg cagacgcaga tggccgagct gaacaaccag
     3841 gtgaagaagg aagcggatgc gaatcgcgcg ctgcaggcga agctcggcaa ccgccgggtg
     3901 gacgtgacca gcgccgccgt ccgccaaggc gacgggcgga ttgtccgcat ccccggcaac
     3961 ggcatctgct acatcaacct gggccagggc gaccaagtca cgccgggcat gacgttcgaa
     4021 gtatatgacc aggccgaagg cgtgccgccg atcccgcaga acgcgacggg cgacgagcag
     4081 ctgccggtgg ggaaggcgtc gatcgaaatc acccgcgtgg gcacgaccag cagcgaatgc
     4141 cgcatcgtcc gcacatcacc gggcgcggtg ctcagcgaag gcgacgtcat cgccaacctc
     4201 gtgtacgacc cgaacaacaa gtacaacttt ttcgtgtacg gcaacttcga cctggccaac
     4261 accggccggc caaacccggc cgatggtgag gtgatcaaac gcctggtcac gcaatggggc
     4321 gggaaggtcg tcgaccaggt gaacgtggac accgacttcg tcgtgctggg caaggaaccg
     4381 acgctgccga gctattcgaa ggaagaattg gaatccccgc tgaatgccga caagctcgcc
     4441 aaggcccagg cggagttgga gaagtatcac gagatcatgc agacggcccg cgatctgcac
     4501 atcccgattc tgaaccagaa ccggttcctg tattacatcg ggtactacga ccaggcgaag
     4561 cgctaaatcg cgctgcccac aaacgcgccg tgtggcaccc gccgcttgcg gcttggcttc
     4621 ttttcagatt cagaaacaga cgctaagccg caagcggcaa acggttcagg cgtcacgcgc
     4681 cgctccaccg acttccacca cccggcacgg cggcagggac tgcaggaaca ttcgcccgta
     4741 gcgcttggtc ttcacccggc tgtcgaggat cgcaacgatt cccttgtccg tcttgctgcg
     4801 gatcagccgg ccgaaaccct gttttaactt gatgatcgcc tccggcaccg agtattcgat
     4861 gaacggattc ccgccggcgc gctgcacggc ttccagccgg gcttcaacga ggggttcatc
     4921 ggggaccgcg aaggggagct tgacgatgat gacgtttcgc agggcgtcgc cctgcacgtc
     4981 gatgccttgc cagaagctgg cggtgccgaa cagcaccgcg ttggcggtgg cgcggaagcg
     5041 gtcgagcagg atttttcgcg gcgcgttctg gccctgcacg agcagcggat agccgagttc
     5101 ttgcagtcgg ctgttgagcc ggttggcggc gtcgatcagc atcttatagc tggtgaacag
     5161 cacgaacgcc ccgccgttgg tttgctggag gtagtggatg atgcgatcgc acgcggcggg
     5221 gagaaagcgc agcgtgtcgt tgggctcggg caggtcggtt tcaatgtaga gcgtggcttg
     5281 cgcttcgtag tcgaacggcg agccgagggc gagggttttg catttctcga cgccgaggcg
     5341 cgactggatg tatgcgaatg aagtagcacg ggcgtcccgc ccgtgcctgg cattgtttga
     5401 caccggcgag acgcccgtgc tacgttcgac ggctgtatcc gccatcgtgt tgccgtcgat
     5461 ccgccagcgc cacttccagt tcttcaatcc ggcttcgtcc ggattgttcc acgcgtaatc
     5521 ggcgcagtga tagaggtccg gctcatcacg gacgagatgg tcgtatgttt ccgtttgcca
     5581 cacttcgccg ctgcgcttga gcgctttgtt gatggcgtgc gccgtgaacg acttccaaga
     5641 atgcaggatc ttcgccagat cgaagtcgcc gaccggctgc accagcgcat gcacgtggtt
     5701 tggcatgatg caccacgcga gcagacggta acgcacgcca tcgaacttac gcagcgcgcc
     5761 ggcgacgatc gcggcggcac ggctgtcgcg caggagacac tcgccatacc cggcatcgag
     5821 atacttctgc actttttcgg agtgaagatc ccgcagctga tcgacttcga cggccgtcat
     5881 ttcgcgtccc gcggagacgg cgcgcttcgc gatctcctcg cgctcggcga cccatccatc
     5941 cagcacgttc tgaggcagtg aatcgccgag gcggaacgtc acggcatata cggcgccgaa
     6001 acgcgtccag tgcggcaggt gagcgccttg gcgaaccgag atggcgctgg catcccgtgg
     6061 ctcgcttggc acgggcgaga cgcccgtgct acggggggcg gcatcctgcg tagcacgggc
     6121 gtctcgcccg tgccggggtg cgctgcttcc gctcgacgcg cagagcgtcg ccgacgtcat
     6181 caccacgcta tgcaccttct caaacagata ccgcttcaac ccctcggcaa cattcaccgg
     6241 cgcggcatgt aagctcacgc gcttcggcgt tcggccgttc acgtccatcc aatacaccgc
     6301 gtccggcatc gtttgcttca ggatcgaatc ggtcacctcc gcaaagttcg cgatcttcgc
     6361 cgcggccgcc gtcagctcgc tgatctcttc ttccggctcg atgtgcgtca gcatcgcctt
     6421 gatgtgaagc gacaggtcgc gcagcttcgg cgtcaggtcg ttctcgatga tctccttgtc
     6481 gcgcacccgg ccgttgccgc gaccgaactg ctcgtgccat cgcgcgcagc ggtcgaagaa
     6541 gccttcggac agatcgttca gctcgacgat gtcgcggatc gcgtcgttgg cgcacgaccc
     6601 gtgtacgctt agcagcccgc ggcctttctt cacgtcgtac agcgcgcgaa ggttatagcg
     6661 caggccggct tcgctgatct tcaagccgaa atgctggccg gccacatctt ccaccgtgtg
     6721 ggcttcgtcg aggatgacga cttcgtactt gggcaggtaa ttca
//
LOCUS       DATIMH010000486         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3002975, whole genome shotgun sequence.
ACCESSION   DATIMH010000486 DATIMH010000000
VERSION     DATIMH010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3002975"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VLI90_08505"
     CDS             complement(<1..1219)
                     /locus_tag="VLI90_08505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV14287.1"
                     /translation="MNSPEPGLRSHITLFLCTILHAFTHAYGSMFVPLYLLITADLHL
                     SGVKQASLLVALYTLFYCICSYGAGLLADRFSRKLILGIGLLGNAIAIILIGLTRQYQ
                     LLLLWAILAGICGTLFHPAASGLVPAHYPKAPGMAIGLLGIGSGVGFFVGPEYSGWRA
                     QVASWSWHGWQIAQWQKPCIELGIAGLIVGVIFLLIAKDVHDGAISPRSGNPAGARVS
                     SVEIKETRAPAGLPLRGEQARAPLGCAMSIRVAVVGAVLGCRDFAGVGGLTLAGIYLQ
                     KAHGFDTKHTGAVLGTMMLLSVIVNPTLVWLTGGRKRLPALRGVLILGGAIAATVPFF
                     SAIFALPVLCAFQTCQAGSYAISDAALLERVPARSRGRVYGLYFTLAGTLGAAGPWIM
                     GFWTDAMGSRAAMA"
     gene            complement(1303..>2480)
                     /locus_tag="VLI90_08510"
     CDS             complement(1303..>2480)
                     /locus_tag="VLI90_08510"
                     /inference="COORDINATES: protein motif:HMM:NF020939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HSV14288.1"
                     /translation="RKAVDEVTALLNERLKDRAIFAYHAGMDQAARTANQEQFMQTDR
                     AIAVATNAFGMGINKPDIRLVVHYNMPGTLEAYYQEAGRAGRDGLPARCVMLFSFQDR
                     RMQEFFIDKIGQENPGTDPAVIEELKTHATRKLELMLRYGQTHDCRRRMILDYFGDSA
                     EVHGCNCDVCRRNHGDIEVTAGPVVDEEVTLLIRQMLSAIARLNGKFGVGAIAEVLVG
                     ERSERTQRWGLETLSVFGLLKLYGTKPVIAMLHRLLEAGLARQRRLGDQRFGSVVELT
                     AAGVGVMKGTQPPPGTLVDLKPSARRRRETKVTAQDAEEEQIDPETMARFEKLRAARL
                     ELSRAKQLPPYVICHDSTLKTIARIVPADLESLQQIKGMGPYKVQEYGQALLNALNG"
BASE COUNT          449 a          828 c          788 g          415 t
ORIGIN      
        1 acgccatcgc cgcgcggcta cccattgcat ccgtccaaaa ccccatgatc catggccccg
       61 cggcgccgag cgtgccggcg agcgtgaagt acaggccgta cacgcgacca cgcgatcggg
      121 caggaacgcg ctccaacagc gccgcgtcgc tgatcgcgta gctgccggcc tggcacgtct
      181 ggaacgcgca cagcacgggc agcgcaaaaa tcgccgagaa gaacggcacg gtggcggcga
      241 tcgcgccgcc gaggatcaac acgccgcgga gcgcgggcag gcgcttgcga ccgccggtga
      301 gccagacgag cgtgggattc acgatgacgc tgagcagcat catcgtgccg agcacagcgc
      361 cggtgtgctt ggtatcgaag ccgtgggctt tctgcagata aatccccgcg agggtgaggc
      421 cgccgacgcc ggcgaaatcg cggcaaccga ggactgcgcc gactactgcg acgcggatcg
      481 acatcgcgca accgagtggc gcgcgcgcct gttccccgcg tagcggtagc cccgcagggg
      541 cacgtgtttc tttgatctcg acggaagaaa cacgtgcccc cgcggggtta ccgctacgcg
      601 gcgatatcgc gccgtcgtgg acgtctttcg cgatgagcag gaaaatgacg ccgacgatca
      661 gtccggcgat gcccagttcg atgcacggct tctgccactg ggcgatctgc cagccgtgcc
      721 agctccagct ggcgacctgc gcgcgccaac ccgaatattc cggcccgacg aagaacccga
      781 cgccggaacc gataccgagc aggccgatcg ccatgcccgg cgccttggga taatgcgccg
      841 gcaccagccc gctggcggcg ggatggaaca gcgtgccgca gatgccggcc aggatcgccc
      901 acagcagcag cagttgatat tgccgcgtga ggccaatcag aatgatcgcg atcgcgttgc
      961 cgagcaggcc gatgccgagg atgagctttc ggctaaaacg gtcggccaac aggccggcgc
     1021 cgtacgaaca gatgcaataa aagagcgtgt agagcgcgac cagcagcgac gcctgcttca
     1081 cgccggacag gtgcaggtcc gcggtgatga gcaggtacag cggcacgaac atggacccgt
     1141 acgcgtgcgt gaacgcgtgc aggatcgtgc ataaaaacag cgtgatgtga cttcgcagcc
     1201 ccggctcggg agaattcatc gaagcgcaac agtagcacgg gcgtctcgcc cgtgccacgg
     1261 tgctcagaca cgggcgagac gcgtgtgctg cgaacgtcgc gttcacccgt tcaatgcgtt
     1321 caacagcgcc tgcccgtatt cctgaacttt gtacggcccc atccccttga tctgctgcaa
     1381 cgattccaag tccgccggca cgatgcgcgc gatcgtcttc agcgtactgt cgtggcagat
     1441 cacgtacggc ggcagttgct tggcgcgcga cagttccaga cgagccgcgc gcagcttctc
     1501 gaatcgcgcc atcgtttccg gatcgatctg ctcttcctca gcatcctgcg ccgtgacttt
     1561 cgtctcgcgc cggcgacgcg ccgaaggctt cagatcgacc agcgtgcccg gcggcggttg
     1621 cgtccccttc atcacgccca cgcccgcggc ggtgagttcg acgacggagc cgaagcgctg
     1681 atcgcccagc cgccgctggc gcgcgagccc cgcttccagc aggcggtgca gcatcgcgat
     1741 gacgggcttg gtgccgtaca gcttgagtaa gccgaagacg ctgagcgttt ccagtcccca
     1801 gcgctgcgtg cgttcgctgc gttcgccgac aagcacctcg gcgatcgcgc cgacgccgaa
     1861 cttgccgttc agccgggcga tcgccgacag catctgccgg atcagcagcg tgacttcctc
     1921 atcgaccacc ggccccgcgg tcacttcgat gtcgccgtga tttcgccggc aaacgtcgca
     1981 gttgcagccg tgcacctcgg cgctgtcgcc gaaataatcc aggatcatcc gccggcggca
     2041 atcgtgggtt tggccatagc gcagcatcag ctccagcttc cgcgtcgcgt gggtcttcag
     2101 ttcctcgatc acggcgggat cggtgccggg attttcctgg ccgatcttgt cgatgaagaa
     2161 ctcctgcatc cgccggtctt gaaagctgaa gagcatcacg cagcgcgccg gcaatccatc
     2221 gcggccggcg cggccggcct cctggtaata cgcctccagc gtccccggca tgttgtagtg
     2281 gacgaccagg cggatgtcgg gcttattgat gcccatgccg aatgcgtttg tagcaaccgc
     2341 aatcgcgcgg tcggtctgca tgaactgttc ctggttggcc gtccgcgccg cttggtccat
     2401 accggcgtga tatgcaaaga tcgcgcggtc cttcagccgt tcgttcaaca gcgcggtgac
     2461 ttcatcaacg gccttgcgcg
//
LOCUS       DATIMH010000487         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5026989, whole genome shotgun sequence.
ACCESSION   DATIMH010000487 DATIMH010000000
VERSION     DATIMH010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5026989"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VLI90_08515"
     CDS             complement(<1..526)
                     /locus_tag="VLI90_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14289.1"
                     /translation="MARPKSKKKDSSRPAAAVMGEDAVQFHMHITDVHRGERIAEGFE
                     PITQDTTRGPVEMRYYPVAGARRAAIFVGGTGGGFDTPVKGWLYPRLCTELQGDGIAG
                     LRVRYRYASELEECVLDVLAGISFLEADHVTSIALVGHSFGGAVVIQAAALSPSVRTC
                     VALSTQTFGAAPASE"
     gene            complement(534..728)
                     /locus_tag="VLI90_08520"
     CDS             complement(534..728)
                     /locus_tag="VLI90_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14290.1"
                     /translation="MAGKTSPTKMSRGKVTTKESPGGSPKAGRRQSRAERDDTPEARA
                     QQVRAPKTHPGRAPASRRKD"
     gene            1043..>3281
                     /locus_tag="VLI90_08525"
     CDS             1043..>3281
                     /locus_tag="VLI90_08525"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HSV14291.1"
                     /translation="METLESRTLMAGDGAIRINAGGGAYTDLAGHAWSADMDFTGGTV
                     SNGRYPVAGTSDDPLFYTRRWGTETYNIPLANGSYTLNLYFAEPVMTAAGKRVFNVSA
                     EGQPLLTNLDLFVAAGYRNSYVRSFPISISDGALNIGFTAITENPIISAIEVIPADVT
                     PSAPAAPTSLAASAITTFTAHLTWSEATYPAATSFTVERLDPGDSDYHSLGTTTSKFF
                     DDTGMSDGAVYSYRVRAANDAGTSDPSDSITITTLQQLASPWSQMDIGSVAKAGSATV
                     AFDTGTFTVSGGGADIWNGADAFHYVYQPLVGDGSIVAHLASQQNTNGWAKSGVMIRE
                     SLNADSRFADMVLTPSNGVSLQRRTSTGLGSVSSTTSAGKGWWVKLVRTGSTITGFAS
                     SDGQNWKLVGSTMLPMANNVLVGLCVTAHDNTKLNTSTFDHVAVTTTGNASSIWTAGT
                     PAPLTRWESQTFTYNGKMYIFGGFYNHAIQATTRCDVYDPATDAWTQLATQLPIPITH
                     AGVAQIGSVVYFAGGYVGDWTKAPATNRVFAYDIDANTWTELPTLSGARVAGGLVALG
                     NVLHFYGGLNTTRTADMADHWAFDLDNPSAGWVSKAPMLNPRNHIGYAAVNGIAYAIG
                     GLHVMNERSGQDAEVDAYDTATDKWTKVASLPFAWSHFLESTMVVNGKIVIVGGQANG
                     GGGGAYVPTIAAYDPDADQWQTLTPLPEARQAASAVWIDGKLYVEGGATQDSLGEGFP
                     QQQLWF"
BASE COUNT          580 a         1081 c         1012 g          608 t
ORIGIN      
        1 gctcgctcgc gggggccgcg ccaaaagtct gcgtcgacaa cgcgacgcag gtccgcacgc
       61 tcggcgacaa cgcggccgcc tggatgacca ccgcgccacc gaaggaatgt ccgaccagcg
      121 cgatcgacgt gacgtggtcg gcctccagaa agctgatgcc tgccagcacg tccaacacgc
      181 actcttccaa ttcactggca taccgatagc gcacgcgcaa gccggcgatg ccgtcacctt
      241 gaagctccgt gcacagccgc gggtacagcc agcctttaac tggcgtatcg aagccaccgc
      301 cggtgccacc gacgaagatg gcggctctcc tcgcgccggc taccgggtaa tagcgcatct
      361 caacggggcc gcgcgtcgtg tcttgcgtga tcggctcgaa tccctcagcg atgcgctcgc
      421 cgcggtgcac atcggtgatg tgcatgtgga attgaaccgc gtcttcgccc atcaccgccg
      481 ccgcgggcct ggatgaatct ttcttcttcg atttcggcct tgccatcgtc cgctcaatcc
      541 ttgcgacggg acgccggcgc acgacccggg tgcgtcttgg gcgcgcgaac ttgctgcgcg
      601 cgcgcctcgg gcgtgtcatc acgctcagcc cggctctggc ggcggccagc cttggggctg
      661 ccgccggggc tttccttggt ggtgactttg ccgcgtgaca ttttggttgg actcgtcttg
      721 cctgccatgg catctccttc ttcacaatag actcgagtct cgcccgcagc gccggtgcca
      781 atgctcgccg atgtgccctt ccagacggca aatctcccac aggttctgaa gattccgata
      841 acgtccacgc gtggtctttc gtgacggctt tgccgtttgt agtgaccgct ggcttctcgc
      901 taaccgtcgc cggcgctaat ttccgttggc cgtcctgagc aggcgggata aacttcccgc
      961 actcgccgtc atcattttgc gtcagggacc tatgacttgt tcacgccaga gaccgttgat
     1021 taagcgcgga agttctgcgc ggatggagac ccttgagtcc cgcacgctga tggccggcga
     1081 cggggcgatc cgcatcaacg ccggcggcgg ggcgtatacc gaccttgccg gtcacgcctg
     1141 gtcggcggac atggatttca ccggcggcac cgtcagcaac ggcaggtacc ccgtggcggg
     1201 gacgagcgat gatccactct tctacacgcg tcgatgggga actgagactt acaacatccc
     1261 gctcgccaac ggttcttaca cgctgaacct gtacttcgct gagccggtga tgactgccgc
     1321 cggcaagcgc gtcttcaacg tcagcgctga aggccaaccg ctgttgacca acctggacct
     1381 gttcgtggcc gcgggatatc gcaattcgta tgtgcggtcg ttcccgatct cgatcagcga
     1441 cggcgcgctg aatatcggct tcaccgccat caccgaaaat ccgatcatct ccgccatcga
     1501 ggtgattccc gcggatgtga caccgtccgc gccggcggca ccgaccagct tggcggcgtc
     1561 ggccatcacg acgttcaccg ctcatctcac ctggagtgaa gccacatacc ccgcggcgac
     1621 ttcgttcacg gttgagcgcc tggaccccgg cgacagcgat tatcattcgc tcggcaccac
     1681 gacctcgaaa ttcttcgacg acaccggcat gtccgacggc gcggtctact cgtatcgcgt
     1741 gcgcgccgcc aacgacgccg gcacttcgga tccatccgat tccatcacga tcaccacgct
     1801 tcagcagctg gcgtcgccgt ggtcgcagat ggacatcggc tccgtcgcca aggcgggcag
     1861 cgccacggtc gccttcgaca ccggcacgtt caccgttagc ggtggcgggg cggacatctg
     1921 gaacggcgcc gacgcgtttc actatgtcta ccaaccgctc gtgggcgacg ggtcgatcgt
     1981 tgcccacctc gcatcgcagc agaacaccaa cgggtgggcc aagtccggcg tcatgatccg
     2041 cgaaagcctc aacgccgact cgcgcttcgc ggacatggtg ctgacgccgt cgaatggcgt
     2101 gtcccttcag cggcgaacat cgaccggcct tggttcggtc agctcgacga ccagtgccgg
     2161 caagggctgg tgggtgaaac tcgttcgcac cggcagcacg atcaccggct tcgcttcgag
     2221 cgatggtcag aattggaaac tcgtcggcag cacgatgctg ccgatggcca acaacgtgct
     2281 ggtcggcctg tgcgtcaccg ctcacgacaa cacgaagctc aacacgtcaa ccttcgatca
     2341 tgtggcagtg acgacgaccg gcaatgcgtc gtccatctgg acggccggca caccggcgcc
     2401 gctgacgcgg tgggaatcgc agacgttcac ctacaacggc aagatgtaca tctttggcgg
     2461 gttctacaac cacgccatcc aggcgacgac gcgctgcgat gtgtacgacc cggccaccga
     2521 cgcctggacg cagctggcga cgcagctgcc gattcccatc acccacgcgg gcgtcgcgca
     2581 gatcggttcg gtcgtctatt tcgccggcgg atatgtcggc gattggacca aggccccggc
     2641 gacgaatcgc gtcttcgcct acgacatcga cgccaacacc tggactgaat tgccgacgct
     2701 gtctggtgcg cgcgtggccg gcggattggt ggcgctgggc aacgtgctgc atttttatgg
     2761 cggcttgaac acgacgcgca cggccgacat ggcggaccat tgggcgtttg acctggataa
     2821 tccatcggcc ggttgggttt cgaaggcgcc gatgctgaac ccgcgcaatc acatcggcta
     2881 cgccgccgtc aatggcatcg cttacgcgat cggcgggctg cacgtcatga acgagcgcag
     2941 cgggcaagat gccgaggtgg atgcgtacga cacggcgacg gacaagtgga cgaaggtggc
     3001 gtcgctgccg tttgcatggt cgcacttcct cgaatcgacg atggtcgtca acggcaagat
     3061 cgtcatcgtc ggcggccaag ccaacggcgg cggtggtggg gcatacgtgc cgacgatcgc
     3121 ggcgtacgat ccggatgccg atcagtggca gaccctaacg ccgctacccg aagcccgcca
     3181 agccgcgtcg gcggtgtgga tcgacggcaa gctctatgtc gaaggcgggg cgacgcagga
     3241 cagtctgggt gaaggttttc cgcagcagca gctgtggttt a
//
LOCUS       DATIMH010000488         3581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_405081, whole genome shotgun sequence.
ACCESSION   DATIMH010000488 DATIMH010000000
VERSION     DATIMH010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405081"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VLI90_08530"
     CDS             complement(<1..907)
                     /locus_tag="VLI90_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV14292.1"
                     /translation="MNRTSVGLKATSLAPVGDVSPEPLCTASATATFGTATTAVEATG
                     PAMRPVRQLDRAAYEDVKPKLDRRYVTAALMLVMVLASMEQTVTSTAMPTIIGELHGL
                     QHYSWVASIYLLACTVSMPLYGRLADALGRKRVILFAIAVFLVASLAAASAHTMTQLI
                     IYRGLQGLGAGGIMPVVLTILGDIFTLEERARIQGFFSAVWGTASLAGPALGAFLVNT
                     FGWRSIFFVNLPFGVIAATVLAWKYHDLEKPHSTDLDMPGAISLALGCTALLALVSGM
                     GPDGFPFGLDMALLAIAVAAAAHFVV"
     gene            1099..2325
                     /gene="trpB"
                     /locus_tag="VLI90_08535"
     CDS             1099..2325
                     /gene="trpB"
                     /locus_tag="VLI90_08535"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HSV14293.1"
                     /translation="MSTASAPTVNLPDATGHFGAYGGVFVPETLVSALTQLEAEYVKA
                     KNDPAFSREFDYYLREFVGRPSRFYFARRLTEAWGGAKIYLKREDLNHTGAHKINNTI
                     GQALLTKRMGKQRVIAETGAGQHGVATATAAALFGLKCDVYMGSEDIRRQNLNVFRMR
                     LMGAGVLEVTSGSKTLKDATNEAMRDWMGSVEHTHYIIGSVVGPHPFPMIVRDFQSMI
                     GTETRAQCLETEGRLPDYVVACVGGGSNAAGIFAPFANDKEVKLVGVEAAGRGTSLGQ
                     HAATLCYGRPGVLHGCLTYVLQDDDGQTADVHSASAGLDYPGVGPEHSFWKDAGRVEY
                     TCISDDEALAAFQQVAQLEGILPALESSHAFAQAAKIAAKLPKDKIVVINLSGRGDKD
                     CQEVARLLEERQAAKR"
     gene            complement(2446..3471)
                     /gene="gap"
                     /locus_tag="VLI90_08540"
     CDS             complement(2446..3471)
                     /gene="gap"
                     /locus_tag="VLI90_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HSV14294.1"
                     /translation="MAIKVAINGFGRIGRLVFRAGVKNPNIEFVGINDLVPPDNLAYL
                     LKHDSTHGRFNGTVEARADGIVVDGKFVPCVSIRNPAELPWGKNHASYVVESTGLFTD
                     YAGAENHLKAGAKRVVLSAPTKDPEKVPTLLIGVNEKKFDPANHKVVSNASCTTNCLA
                     PVAKVINDNFGLAEGLMTTIHATTATQPTVDGPSKKDWRGGRGAGQNIIPAATGAAKA
                     VTLVLPELKGKLTGMAFRIPTPDVSVVDLTFKTAKSTSLKEINAAMKAASEGELKGIL
                     GYTEDEVVSTDFVTDPRSSIFDAGAGIELNANFFKVVSWYDNEWGYSNRVIDLLTVMA
                     KRDGLLK"
BASE COUNT          678 a         1120 c         1136 g          647 t
ORIGIN      
        1 gcacgacgaa gtgcgccgcc gccgcgacgg ctatggcgag cagcgccata tcgaggccga
       61 acggaaatcc atccggtccc atgccggaga ccagcgcgag cagtgcggtg catccgagcg
      121 cgagcgaaat cgcaccgggc atgtcgagat cggtcgaatg cggcttttcg agatcgtgat
      181 atttccacgc cagcaccgtt gccgcgatca caccgaacgg caggttgacg aagaagatcg
      241 atcgccagcc gaatgtattg acgagaaacg cgcccagcgc cggcccggcg agagacgcgg
      301 tgccccagac ggcgctgaag aaaccttgga tgcgggcgcg ttcttcgagc gtgaagatgt
      361 cgccgaggat cgtcagcacg accggcatga tcccgccggc gccgagcccc tgcaggccgc
      421 ggtagatgat cagctgcgtc atcgtgtgcg cggatgccgc cgccagcgac gcgacgagga
      481 agacggcgat cgcgaacagg atcacgcgtt tgcggccgag cgcatcggcc aatcgtccgt
      541 acagcggcat gctgacggtg caggcgagca ggtagatcga cgcgacccag gagtaatgct
      601 gaaggccgtg cagttcgccg atgatcgtcg gcatcgccgt gctggtgacg gtctgctcca
      661 tcgacgcgag caccatcacc agcatcagcg ctgccgtgac gtaccggcgg tcgagctttg
      721 gtttgacgtc ttcgtacgct gcgcgatcga gctggcgcac cgggcgcatg gccggcccgg
      781 tcgcttcaac agcggtcgtc gcggtgccga atgttgctgt ggccgatgcc gtacagagcg
      841 gctcggggct tacgtcgccg acgggcgcca gcgacgtggc cttcaatcca acggatgtcc
      901 tgttcatggt gctgccgatt cccttgaggc cgatcctagg cgcgtcctgc cacgtgcatc
      961 cagataatgt tgcaggcttc gaaaatccgc cggctttaag gctgcgctct tgctgagtcg
     1021 atggcgtgat cgacatgcac gattacgtgc accatgggct ggaagcccat gccactgttc
     1081 ccctaccatt atcggcatat gagcaccgct tccgcgccca ccgtgaatct gcccgacgcc
     1141 actggccatt tcggggccta cggcggcgtt ttcgtgcccg agacgctcgt ttccgccctc
     1201 acgcaattgg aggcggaata cgtcaaggcg aagaacgatc cagcgtttag tcgcgagttc
     1261 gattattacc ttagagaatt tgtcggccgg cccagccggt tctacttcgc gcgccggctg
     1321 accgaagcgt ggggcggggc gaagatttac ctgaagcgcg aagacctgaa tcacaccggc
     1381 gcgcacaaga tcaacaacac gatcggccag gcgctgctga ccaagcgcat gggcaagcag
     1441 cgcgtgatcg ccgagaccgg cgcgggccag cacggtgtcg ccaccgcgac ggcggccgcg
     1501 ctgttcggcc ttaagtgcga cgtgtacatg ggatccgaag acatccgccg gcagaacctg
     1561 aacgtcttcc gcatgcggct gatgggcgcg ggcgtcctcg aagtcaccag cggcagcaag
     1621 acgctgaagg acgccaccaa cgaagcgatg cgcgactgga tgggcagcgt cgaacacacg
     1681 cattacatca ttggctcggt cgtcgggccg catccgttcc cgatgatcgt gcgcgatttc
     1741 caatcgatga tcggcaccga gacgcgcgca caatgtttgg aaaccgaagg ccggctgccg
     1801 gactacgttg tcgcctgcgt cggtggcggc agcaatgccg ccggcatctt cgcgcctttc
     1861 gcgaatgaca aggaagtgaa gctcgtcggc gtcgaagccg ccggtcgcgg cacgtcgctc
     1921 ggccagcacg ccgccacgct ctgctacggt cgccccggcg tcttacacgg ttgtctcacg
     1981 tatgtattgc aggacgacga tggccaaacc gccgacgtgc actccgccag cgccggcctg
     2041 gattaccccg gcgtcggccc cgagcacagc ttctggaaag acgccggccg ggtggagtat
     2101 acctgcatca gcgacgacga agcgctggcc gcgtttcagc aagtcgcgca gctcgaaggc
     2161 attttgcccg cgctggagtc atcgcacgcg tttgcccaag ccgcgaaaat cgcggcgaag
     2221 ctgccgaagg ataagatcgt ggtgatcaat ctctctggcc gcggggacaa ggactgccag
     2281 gaagtggcga ggctgctgga agagcgacag gccgcgaaac gatgatagca aaaatcgcac
     2341 ggttgaaccg cgagttgccc gcgtagcggt agcgaaataa gcgagcgggg cgatctggtg
     2401 atcgccccgc tcgtgttttt actgaatgcc tacgcaaccc gcgaattact tcagcaaccc
     2461 atcccgcttc gccatcaccg tcaaaaggtc gatgacgcgg tttgagtagc cccactcgtt
     2521 gtcgtaccag gaaacgacct tgaagaagtt cgcgttcagc tcgatgccgg cgccggcgtc
     2581 gaagatgctg ctgcgcggat cggtcacgaa atccgtcgag accacttcgt cttccgtgta
     2641 gccgaggatg cctttcagct cgccttcgct ggcggccttc atggcggcgt tgatttcctt
     2701 caggctcgtg ctcttggcgg tcttgaacgt cagatcgacc accgacacgt ccggcgtcgg
     2761 gatgcggaag gccatgccgg tcagcttgcc cttcagttcc ggcagcacca gcgtgacggc
     2821 cttcgccgcc ccggtggcgg cggggatgat gttctgaccg gcgccacggc cgccgcgcca
     2881 atccttcttg ctcgggccgt ccacggtcgg ctgcgtcgcc gtcgtcgcgt ggatcgtcgt
     2941 catcaatcct tcggccagcc cgaagttgtc gttgatgacc ttcgccaccg gcgccaggca
     3001 gttggtcgtg caggaagcat tcgacacgac tttgtggttt gccgggtcga acttcttttc
     3061 gttcacgccg atcagcagcg tcggcacttt ttccgggtcc ttagtcggag ccgacagcac
     3121 gacgcgcttc gcgccagcct tcaggtgatt ctccgcgccg gcgtaatcgg tgaagaggcc
     3181 ggtcgattcg acgacgtaag aagcgtgatt cttgccccac gggagctcgg ccgggttgcg
     3241 gatcgacacg caggggacaa acttgccatc gaccacgatg ccgtccgccc tggcttcgac
     3301 ggtgccattg aaccggccgt gcgtcgaatc gtgcttgagc agatacgcga gattgtccgg
     3361 cggaacgaga tcattgattc cgacaaactc gatgttcgga ttcttcacgc cggcgcggaa
     3421 gaccagtcgc ccgatgcgtc caaacccgtt gatggcgacc ttaatggcca tggcaatgct
     3481 ccaaagatgt tcgaaaaagc cgtaacacaa gcgctcgccg gcaccgccag gcgagtcgcg
     3541 aaatctagga gtgtcgcgga ggcatgtcaa gacagcgggc t
//
LOCUS       DATIMH010000489         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4739348, whole genome shotgun sequence.
ACCESSION   DATIMH010000489 DATIMH010000000
VERSION     DATIMH010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4739348"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VLI90_08545"
     CDS             <1..250
                     /locus_tag="VLI90_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14295.1"
                     /translation="ENGVLKLDQPIALEEHARVTVAIAEASDRAATAPNATAEQLRQS
                     LPDDFFLKDVIGIGEGPENGDGAAEHDHYLYGFPKKKK"
     gene            complement(369..626)
                     /locus_tag="VLI90_08550"
     CDS             complement(369..626)
                     /locus_tag="VLI90_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14296.1"
                     /translation="MIVAGADVGQAKLLHHDERGEIDEGDVRFVRVTAAHLPSAVETV
                     RRDARQFLRAIRDGRNDLRDVRLRRRRANLRVDASDRLLST"
     gene            689..1381
                     /locus_tag="VLI90_08555"
     CDS             689..1381
                     /locus_tag="VLI90_08555"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HSV14297.1"
                     /translation="MLAPMLHFLADATDMPPSAWHDMFHLTLPIVEKILRTIIVYFAL
                     MLGLRISGKRELAQLNPFDLIVLLMLSNTVQNAIIGDDNTVTGGIIGAITLLSVNYLI
                     VRLVYRSPMLQRFFGGEAEVLVRHGHIRKHKLEQELITPEELEAAAHRQGISSLTDVE
                     KCILEPTGTISFIERKPTTDEERHEQLVKQIEQLTQIVTKRRAAMAARANGRVGEYRG
                     ASRRNGSARSSS"
     gene            1502..2005
                     /locus_tag="VLI90_08560"
     CDS             1502..2005
                     /locus_tag="VLI90_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HSV14298.1"
                     /translation="MKLDVVPWYNQGLKFSCTCCGNCCTGGPGYVWISEEEIRLLAAH
                     LKITPEETVEQYCRKINGKFSLNERRSTGGNYDCVFLKEIKPPRSSRKGETIVQAKRV
                     CGIYQVRPLQCRTWPFWPENVESKQAWNAETRKCPGMNTGKHYTPGQIESLRDAEAWP
                     EKSPGSK"
     gene            complement(2030..3550)
                     /locus_tag="VLI90_08565"
     CDS             complement(2030..3550)
                     /locus_tag="VLI90_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV14299.1"
                     /translation="MSIDVERPGCWRRVAPLVVLLLSASIAQSATTAASTTQFATLTL
                     SDGRTTVTLFKPADFTESKASSPTQRVLETNDSSTRLTILPEDQPTDPASAAAFVENT
                     KQSLTAARGADLLSAPATEQVENFPLVMHYRVRRAPEQIVDTTVQWRRVGPVMVQAVS
                     EVRSQSATTIDEVKNGVAEMLKRAYVEGYQEPTAKNATAAGVDANAAAGASVDAGPNV
                     QRFAGDTVEFTVPPFFEQIGIAKSDTSRDYLSSDHNTELVVTLMPAGTTLDYASGPIQ
                     LAAAQRELNAPAASAIKPTTAPVLLKNTALPVEIQWAFDRDGKPATGFRMFRKIHPKQ
                     VVSCQITTTDASSKAQHLGETTLNSMKSLLPPDPPPVAKSHGAGGPNEEKRNQRAQAA
                     LDQAKKLADAKKDDEAYAKYKYIITEYPDSPSAAEAKTAMDKYEADPAFAERHKKPSE
                     ASPADKAASMLALADNYSYVGRQDEARAKYQQVIKEYPNTPAAEKAKKRLVDIGKR"
BASE COUNT          692 a         1125 c         1175 g          684 t
ORIGIN      
        1 cgaaaacggc gttctgaagc tggaccagcc gatcgcgctg gaagaacacg cgcgcgtgac
       61 ggttgccatc gccgaggctt ccgatcgcgc ggccactgcg cccaacgcca cggctgagca
      121 gctgcgccaa tcgctgcctg acgacttctt cctcaaggat gtcatcggca tcggcgaagg
      181 tcccgagaac ggcgacggcg cggcagagca cgaccattac ctttacggct tcccaaagaa
      241 aaagaagtga tcttcatcga cacatggggt tgggtagcgc tggcggatcg caaagatcag
      301 tggcacacgc cggcgttagc cgagatcaaa cggctcgttg agctcggcga gcaattcatc
      361 accagcgact atgtgctcag taaacgatca ctcgcgtcta cacgcagatt ggcgcgacgg
      421 cggcggagcc gtacatcgcg aagatcgttt ctgccgtcgc ggatggcacg caggaactgg
      481 cgcgcatcac gccggaccgt ttcgaccgcg cttggcagat gcgccgccgt tacgcggaca
      541 aaccggacat ctccttcgtc gatttcacct cgttcgtcgt gatgcaggag cttggcctga
      601 ccgacgtctg caccggcgac gatcatttcc gaaagtgggt ctcggctttc gcacgctgcc
      661 ggaaccgtag ctatcgaggg tttacaacat gctggcaccg atgcttcatt tcctcgccga
      721 cgccaccgac atgccgccgt ctgcctggca cgacatgttt cacctaacgc tgccgatcgt
      781 cgagaagatc ctgcgcacga tcatcgtcta cttcgcgctg atgctgggcc tgcgcatcag
      841 cggcaagcgc gagttggcgc agctcaatcc gttcgacctg atcgtgctgc tgatgcttag
      901 caacacggtg cagaacgcga tcatcggcga cgacaacacg gtgaccggcg gcatcatcgg
      961 ggccatcacg ctgctgagcg tcaattacct gatcgtccgc ctggtctacc gcagcccgat
     1021 gctgcagcga tttttcggcg gcgaagcgga ggtgctcgtc cgccacgggc acatccgcaa
     1081 gcacaagctg gagcaggaac tgatcacacc ggaagagctg gaagcggcgg cccatcggca
     1141 gggcatctcg tcgttgaccg atgtcgagaa gtgcatcctg gaaccgacgg gaaccatctc
     1201 gttcatcgag cgaaagccaa ccacggatga agagcggcac gagcaactgg tgaagcagat
     1261 cgagcagttg acgcagatcg tgacgaagcg gcgcgcggcg atggcagcac gcgcgaacgg
     1321 ccgcgtgggt gaatatcgcg gcgcgagcag gcgcaacggc agcgcgcgtt cgagcagttg
     1381 atagccgcag gcgacagcca gcgggtgcgc tcgacgatga aaccacgttc agaatccgaa
     1441 gcccccgctg gctgtcgcct gcggctacta tcgcacgctc tcgcggcgta caatcgcccc
     1501 catgaagttg gacgttgttc cctggtacaa ccagggcctg aaattcagct gcacctgctg
     1561 cggcaactgc tgcaccggcg ggccggggta tgtgtggatc agcgaagagg agatccgcct
     1621 gctggccgcg cacctgaaga tcacgccgga agaaacggtc gaacaatatt gccgcaagat
     1681 caacggcaaa ttcagcctga acgagcggcg atcgaccggc ggcaattacg attgcgtgtt
     1741 tctgaaagag atcaaaccgc cgcgatcttc gcgcaagggc gagacgatcg tgcaggcgaa
     1801 gcgcgtgtgc ggcatctatc aagtgcggcc attgcaatgc cgcacgtggc cgttttggcc
     1861 ggagaatgtg gaatcgaagc aggcgtggaa cgcggagacg cgcaagtgcc cgggcatgaa
     1921 cacgggcaag cattacacgc cagggcagat cgagtcgctg cgggatgccg aagcgtggcc
     1981 ggagaaatca ccggggtcca agtgaccggg cacgacagcc gaagtttgat caccgcttgc
     2041 ctatatccac caatcgcttc ttcgctttct cagcggcggg ggtattgggg tattccttga
     2101 tcacctgctg atacttcgcc cgcgcttcgt cctggcggcc tacatacgaa tagttgtcgg
     2161 ccagcgccag catcgatgcg gccttgtcgg cggggcttgc ctcactcggc ttcttgtgac
     2221 gctcggcgaa ggcggggtcg gcttcgtact tgtccatcgc tgtcttggcc tcggcggccg
     2281 acggggaatc gggatactcg gtgatgatgt acttgtactt cgcgtacgcc tcatcgtcct
     2341 tcttcgcatc ggccagcttc ttggcttgat cgagggccgc ctgcgcgcgc tggttgcgct
     2401 tctcctcgtt cggcccaccg gcgccgtgcg atttagccac cggcggcggg tcgggcggca
     2461 gcagcgactt catgctgttc aacgtcgtct cgcccaggtg ctgcgccttg gatgatgcat
     2521 cggtcgtggt gatctggcag ctgaccacct gcttcggatg gatcttgcgg aacatgcgaa
     2581 agcccgtcgc gggtttgccg tcgcgatcga aggcccactg gatctccacc ggcagcgccg
     2641 tgttcttcag cagcacgggc gcggtggtcg gcttgatcgc gctcgccgcc ggcgcattca
     2701 gctcgcgctg cgccgccgcc agttggatcg ggcccgaggc ataatccagc gtcgtccccg
     2761 caggcatcag cgtgacgact aactccgtgt tgtgatcgct ggacagataa tcgcgcgatg
     2821 tgtcgctctt ggcgatgccg atctgctcga aaaatggcgg cacggtgaac tcgacggtgt
     2881 cgccggcgaa acgctgcacg ttcggcccgg cgtccaccga ggcgccggcg gcggcgttcg
     2941 catcgacacc ggcggcggtg gcgtttttcg ccgtcggttc ctgataacct tcgacgtatg
     3001 cgcgcttgag catttcggcc acgccattct taacctcgtc gatcgtggtc gcgctttgcg
     3061 atcggacctc gctcaccgct tgcaccatga ccggcccgac gcgccgccac tgcacggtgg
     3121 tatcgacgat ctgctccggc gcgcggcgga cgcggtagtg catgacgagc gggaagttct
     3181 ccacctgctc cgtcgccggg gccgacaata aatccgcccc gcgggcggcc gttaacgact
     3241 gcttggtgtt ttcgacgaac gccgccgccg atgcggggtc ggtgggttga tcctcgggca
     3301 ggatcgtgag gcgcgtggag gagtcattgg tctccagcac gcgctgagtg ggcgatgacg
     3361 ctttggattc ggtgaagtcg gcgggtttga aaagcgtgac ggtcgtgcgg ccatcactca
     3421 gcgtcagcgt ggcgaattgc gtggtggatg cggccgtcgt cgccgactgc gcgatactgg
     3481 cggaaagcag aaggacaacc agcggcgcga cacgccgcca gcagccgggg cgttcaacat
     3541 cgatgctcat gtaaatatct taggctgtgt ctcacgaacg tgttttgcgc caaggccgcc
     3601 aaggccgcca agacgccaag agcgccaagc agaaggaatt ttcagccaca gatgaacgca
     3661 gatgcacgca gatcag
//
LOCUS       DATIMH010000490         5605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1851350, whole genome shotgun sequence.
ACCESSION   DATIMH010000490 DATIMH010000000
VERSION     DATIMH010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851350"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..728)
                     /locus_tag="VLI90_08570"
     CDS             complement(72..728)
                     /locus_tag="VLI90_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HSV14300.1"
                     /translation="MTQPVDVLARVRAALGRHAPLSAPPIPPEMDEPITRLVHSDFGL
                     PELFARKAMENKIGVELVYVDELAERLIAFLRVQQVQRIALPVSPFLDKLGIPAAIAD
                     GGFFATRWDQMTLDALYDYDAAVTDVYAAVAETGSLVVRASPGHGRALSLVPPIHIAI
                     LQPKDFVPDLVDLLQKLRDDGVTSATSIITGPSKTSDIEMNLVVGVHGPTTVHAFVLQ
                     "
     gene            complement(725..2188)
                     /locus_tag="VLI90_08575"
     CDS             complement(725..2188)
                     /locus_tag="VLI90_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HSV14301.1"
                     /translation="MARRPFGQQAMDEHTEAFLDQAQAARFDFKTASAKAAGDERLKL
                     AVNNAVMRQYSGRSLALMQLPDADRLRTLAGQIKQHALDHLDYYLEQLKANVERNGGQ
                     VHFAADGADARRIILDLAQRANVKTAVKSKSMVSEEIDLLPAMERAGIETFESDLGEF
                     IVQIGHDRPSHIVGPIIHKDRRSIAELFSQYFGTPYNDEPTALTMQARKFLRGKFRRA
                     DLGMTGGNFLVAETGQVCVVENEGNARQSITTPRVLVSLVGIEKIVPRLADLAVVLKL
                     LARSSTGQHMTVYTSLFGGPRGAGESDGPEEFHLVLIDNGRSEILASDYRETLRCIRC
                     GACLNACPVYRSIGGHAYGHVYSGPIGALITPLFQGLANFKELPQASSLCGACYEACP
                     VKIDIPRHLINLRRDIVNQRLLGFWERTIYRLWAWSMLSPLRYAIVVRLQRWRLRRSA
                     RGSGWVTRMPKIASGWTQVRDMPAPAPRTFHQLWSDR"
     gene            2533..3441
                     /locus_tag="VLI90_08580"
     CDS             2533..3441
                     /locus_tag="VLI90_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14302.1"
                     /translation="MATAQTGPDLLVKPWDEGQRVDTSTDGLLNAQGHTREGDNDIRL
                     STYHAIGRWRVLPDNRATPRIGYDVQYWDVQTTDPALPRNLWDMTVGFAQPVAEVNGW
                     FAAVTGAVGYAGNAPFSDSHAVYFAANAIVGREFSKDRALLVALNYNGNRTFYPDVPI
                     PGFAYADRANKYLTYVIGLPYSSITYEPIKGFQIEGGYTLIDTLEAKLAYQFTEHWAV
                     FGQYLDQLSPFHIDATQVDRRLFLHIHEVELGVRYNPTKHIRISLSGGWSFGQEFSRG
                     FDDRSLEPVRHLSDTPFGRLRFELGF"
     gene            3690..4178
                     /gene="moaC"
                     /locus_tag="VLI90_08585"
     CDS             3690..4178
                     /gene="moaC"
                     /locus_tag="VLI90_08585"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HSV14303.1"
                     /translation="MSKLTHLDAKGRAQMVDVGAKPATAREAIAEGFIILKAATLRAI
                     ADETVPKGEVLGTARIAGIIAAKRCGELIPLCHPLPIESVQVEFDLPELDPDHDQPVR
                     LRIKAVTRITAKTGVEMEALTAVSVAALTVYDMCKAIDKSMVIGDIRLLKKTGGTSSN
                     KP"
     gene            4268..4741
                     /locus_tag="VLI90_08590"
     CDS             4268..4741
                     /locus_tag="VLI90_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14304.1"
                     /translation="MRFETTSWIVVLLGWVPAAIGGLVQDHHRLDPGSTATLEQPLGP
                     NSLQLPTKHDNDENIQRPEAVSTPRAPAIAEDRSANRFDLSGTRIDAGVALLEAYAPR
                     LDPSDSLPAPTASFSDTKVTHPAEAPVSVPLPPAFWPGVIALSLCVLFRRVVTSH"
     gene            complement(4733..>5605)
                     /locus_tag="VLI90_08595"
     CDS             complement(4733..>5605)
                     /locus_tag="VLI90_08595"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HSV14305.1"
                     /translation="SGGETLSSAANIFVGQTEAPLMVKPFLAAATNSELMAIMVGGFA
                     NIASGVLGLYTIWLKPFVPDAAGHLAAACFITAPGSLLISKLIVPETDTPVTAAGVQF
                     RVDRIDANLVDAAARGTSEGLGLALNVAAMLISFTALVYLFNALLGVWFGYNPLAHHF
                     HLRQLPVITLQDIFGFVFRPLAWLCGISWQESRTVGSLLGIKTVLNELIAYSQMKDWL
                     ANDPAMLSPRARLLATYALCGFANFASVGIQVGGISSLAPTRRADLSRIGLLAMVGGA
                     IASLMAAAVVGILV"
BASE COUNT         1080 a         1948 c         1613 g          964 t
ORIGIN      
        1 gtatcagcac atcgaggagt atcgcgaagc caaagcccgc gcggccggtg tgtgagcgcg
       61 ctcgctaccc cctactgcag cacgaacgcg tgcacggtgg tcgggccgtg cacgccgacg
      121 accaggttca tctcgatgtc cgacgtcttc gatgggccgg tgatgatcga cgtcgcgctg
      181 gtgacgccgt cgtcgcgcag cttctgcagc agatcgacca ggtccggcac gaaatccttc
      241 ggctgaagga tggcgatgtg gatcggcggg acaagcgata gcgcgcgccc gtggccggga
      301 gaagcgcgga cgacgaggct acccgtctcg gcgacggcgg cgtatacgtc cgtcaccgcc
      361 gcgtcgtaat catatagcgc atccagcgtc atctggtccc agcgcgtcgc gaaaaacccc
      421 ccatcggcga tggcagcggg aatccccagc ttgtccagga atggcgagac cggcagcgcg
      481 atgcgctgca cctgctgcac tcgcagaaat gcgatcagtc gctccgccag ttcatcgacg
      541 tataccagct caaccccgat cttgttctcc atcgccttgc gcgcgaacag ctctggcaaa
      601 ccaaagtcgc tgtgcaccag ccgcgtgatc ggctcgtcca tttctggcgg gatcggcggc
      661 gcgctcaacg gcgcgtgccg tccgagcgca gcgcggactc ttgcaagcac atcgaccggc
      721 tgcgtcatcg atcgctccac agctgatgaa acgtccgcgg tgccggcgcc ggcatgtcgc
      781 gcacctgcgt ccagccggat gcgatctttg gcatccgcgt gacccatccg ctgccgcggg
      841 cgctgcggcg gagccgccac cgttgcaggc ggacgacaat tgcatagcgc agcggcgaga
      901 gcatcgacca cgcccacagg cgatagatcg tgcgttccca gaagccgagc aggcgctggt
      961 tcacgatgtc gcgccgcagg ttgatcaagt gccgcgggat gtcgatcttc accgggcacg
     1021 cttcatagca cgccccgcac aagctgctgg cctgcggcag ttctttgaag ttcgccaaac
     1081 cctggaacaa cggtgtgatc agcgcgccga tcggaccgga atagacatgc ccatacgcgt
     1141 gcccgccgat gctgcgatag accgggcacg cattcaagca cgcgccgcag cggatgcacc
     1201 tcagcgtctc gcgatagtcg ctggcgagaa tttccgaccg gccattgtcg atcagcacca
     1261 ggtgaaattc ttccggacca tccgattcac cggcgccgcg cgggccgccg aagaggctcg
     1321 tgtacaccgt catgtgctgg ccggtcgagc tgcgcgccag cagcttcaac acgaccgcca
     1381 ggtccgccag ccgcggcacg attttctcga tgccgaccaa actgaccagc acccgcggcg
     1441 tggtgatgct ctgccgcgcg ttgccctcat tctccacaac gcacacttgc cccgtctcgg
     1501 cgaccaggaa attcccgccg gtcatgccca gatccgcccg gcggaacttg ccgcgtaaaa
     1561 actttcgcgc ttgcatcgtc agtgcggtgg gttcgtcgtt gtagggcgtg ccgaaatact
     1621 ggctgaacag ctctgcaatc gaccggcggt ctttgtgaat gatcggcccc acgatgtggc
     1681 tcggccgatc gtggccgatc tgcacgatga actcgcccaa atcgctctcg aacgtctcga
     1741 tccctgcccg ctccatcgcc ggcagcaggt cgatctcctc gctcaccatc gacttgctct
     1801 tcaccgcggt cttcacgttc gcgcgctgcg caagatcgag aatgatccgc cgggcatcgg
     1861 cgccgtccgc ggcgaaatgc acctgaccac cgttgcgctc gacgttggcc ttcagttgtt
     1921 ccaggtagta atcgaggtga tccagcgcgt gctgcttgat ctgccccgcg agcgtccgca
     1981 accgatcggc gtccggcaac tgcatcagcg ccagcgaccg cccactgtat tgccgcatca
     2041 ccgcgttgtt gacggccagc ttcagccgct cgtcccccgc cgccttcgcg ctggccgttt
     2101 tgaagtcgaa ccgcgccgcc tgcgcctgat cgaggaacgc ctcggtgtgt tcgtccatcg
     2161 cctgctgtcc aaacggccgc cgtgccatgg cggcggattg tagcgaggcg gcattcgcgc
     2221 ggcgatttct cgatccgatc ccctcgccca cgtactcgtg ggagagggtc agggtgaggg
     2281 tgatttcaag caccagcggc tattgaaatc gcaatcaccc tcacccccgc cctctcccac
     2341 gagacctgcg gtcagcagca cgtgggagag aggcccggaa caacaggcgc gccgagaaaa
     2401 cccgtgcatg tcacgtaaat cgcgcgacat tgcgggcaat ccctcgcgcg acgatactcc
     2461 ccgcccatgc acatccaccg ccgcgggctg gcttggtcac tggggcttct ctccgtcttg
     2521 actctaaatg ctttggccac cgcccaaacc gggccggacc tgctcgtcaa accctgggac
     2581 gaaggccagc gggtggacac gtccaccgac ggtttgttga acgcccaagg ccacacgcgc
     2641 gaaggcgaca acgacatccg cctctccacc tatcacgcga ttggccgctg gcgtgttctg
     2701 cccgacaacc gcgcgacgcc acgcatcggg tacgacgttc agtattggga tgtgcaaacg
     2761 accgatccgg ccctgccgcg aaatctgtgg gacatgaccg tcggcttcgc ccaaccggtg
     2821 gccgaggtga acggctggtt cgccgccgtc accggcgcgg tcggttacgc cggcaacgcg
     2881 ccgttctccg actcccacgc cgtctatttc gccgccaacg ccatcgtcgg gcgcgaattc
     2941 agcaaggacc gcgcgctgct ggtcgcgctg aattacaatg gcaaccgcac gttctatccc
     3001 gacgtcccca tccccggttt cgcctacgcc gatcgcgcta acaaatatct gacctacgtc
     3061 attggccttc cctacagttc catcacctac gaaccgatca agggatttca gatcgaaggc
     3121 ggctatacgc tgatcgatac gctcgaagcg aagctcgcgt atcagttcac cgaacactgg
     3181 gccgtcttcg gccaatatct cgaccagctt agccccttcc acatcgacgc tacccaggtc
     3241 gatcgccggt tgttcctcca catccacgag gttgaactcg gcgtgcgcta caacccgacg
     3301 aagcacatcc gcatctcgct cagcggcggc tggtcgttcg gtcaggaatt ctcccgcggg
     3361 ttcgatgatc gcagcttgga accggtgcgg cacctgagcg acacgccgtt cgggcgactg
     3421 cgcttcgaac tcggattttg acaagcgata tccctccccc gcgtagcacg ggcgtctcgc
     3481 ccgtgtttcg tggtactcta cgaatatcga cggtactccg tcgatgaaat aatcgcgcaa
     3541 gcgaagggtc tgcgaggacc gtcgcttgcg cgattgctgt gtcgatcgag taagatcaac
     3601 gtgcctgagt acatgcacca cgggcaagat gcccgtgcta caaactcgtg cccattcggg
     3661 gctactgctg cgcggctaca atgttccaca tgtcaaagct cacgcatctc gacgccaaag
     3721 gccgcgccca aatggttgac gttggcgcta agcccgccac cgcgcgcgag gcgatcgccg
     3781 agggatttat catcttgaag gccgccacgc tgcgggccat tgccgacgaa acggtgccca
     3841 agggcgaagt gctgggcacc gcacgcatcg ccggcatcat cgccgccaag cgctgtggcg
     3901 aactgatccc gctgtgtcat ccgctgccga tcgaaagcgt gcaagtggag ttcgaccttc
     3961 cggaactcga ccccgaccac gatcagccgg tccgcctgcg catcaaagcc gtcacgcgca
     4021 tcaccgccaa gaccggcgtc gaaatggaag cgctgaccgc cgtcagcgtc gccgcgctca
     4081 ccgtctacga tatgtgcaag gcgatcgaca agagcatggt catcggagac atccgcctgt
     4141 tgaaaaaaac cggcggtacc tcatcgaaca agccataacc cgcccgaaaa ttcgcgcaac
     4201 aaaaaacatc ttgacggagt tcgccccggt gtgatggaat accccgcatc accgaggcat
     4261 ctgtcgtatg cgattcgaga cgacctcatg gatcgtcgtc ctgctcgggt gggtccccgc
     4321 ggccatcggg ggcttggtgc aggaccacca ccgcctcgat cccggttcca cagccacgct
     4381 cgaacaaccg ctcgggccga attcactcca actgccaacc aaacacgaca atgacgagaa
     4441 catccagcgc ccggaagccg tcagcacgcc gcgcgcaccg gcgatcgccg aagaccgatc
     4501 ggcaaatcgt ttcgacctga gcggcacccg gatcgacgcc ggcgtggcac tgctcgaagc
     4561 ctacgccccg cggctggacc catccgattc gcttcccgcg ccaacggcat cgttcagcga
     4621 cacgaaggtc acccaccccg ccgaggcgcc cgtgagcgtc ccactgccgc cggcgttctg
     4681 gcccggcgtg attgcactgt ctctgtgtgt gctctttcgc cgcgtagtca cgtcacacta
     4741 aaatccccac gaccgctgcc gccatcagtg acgcgatcgc cccgccgacc atcgcaagca
     4801 acccgatcct cgaaagatcc gcccgtcgcg tcggcgctaa tgaactgatc ccaccgactt
     4861 gaatgcccac gctggcgaag ttcgcgaagc cgcacagcgc gtacgtcgcc agcaaccgcg
     4921 cccgcggcga cagcatcgcc gggtcgttcg ccagccaatc tttcatctgc gaatacgcga
     4981 tcaactcgtt cagcaccgtc ttgatgccga gcagcgaccc gacggtgcgc gattcttgcc
     5041 acgagatccc gcacagccac gccaggggcc ggaagacgaa gccgaaaatg tcctgcagcg
     5101 tgatgaccgg cagctgccgc aaatggaaat gatgcgcgag cgggttgtaa ccaaaccaca
     5161 cgccgagcaa cgcgttgaac aagtagacca gcgcggtgaa tgagatcagc atcgcggcca
     5221 cgttcagcgc caggccaagc ccttcgctgg tgccgcgcgc agccgcgtcc acgagattcg
     5281 cgtcgatccg atcaacacga aactgcaccc ccgccgccgt caccggcgtg tccgtctccg
     5341 gcacgatcag cttcgaaatc aacaacgaac ccggcgccgt gatgaagcac gccgccgcca
     5401 gatgaccggc ggcatccggc acgaacggct tcaaccagat cgtgtacaac cccaacacgc
     5461 ccgacgcgat gttggcgaag ccgccgacca tgatggccat caactcgctg ttggtcgcgg
     5521 cggccaggaa gggtttgacc atcagcggcg cttcggtctg accgacgaaa atattcgcgg
     5581 ccgacgaaag cgtctccccg ccgct
//
LOCUS       DATIMH010000491         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3581638, whole genome shotgun sequence.
ACCESSION   DATIMH010000491 DATIMH010000000
VERSION     DATIMH010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3581638"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..840
                     /locus_tag="VLI90_08600"
     CDS             136..840
                     /locus_tag="VLI90_08600"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HSV14306.1"
                     /translation="MSAPSPTPTPLQIGLVLFPKVTQLDFTGPLQVFSSLPGAKVHLI
                     WKHIEPVTSDSVLVLTPTTTFADCPQLDVICVPGGFGTDDMINDEEMLAFLRKQAAGA
                     KYITSVCTGSLVLGAAGLLKGYRAATHWTAMDFLSAFGAIPTRTRVCVDRNRVTGGGV
                     TAGIDFALTLVSLLHDRKTAEAIQLRLEYNPAPPFNSGSPDTAPPEVLAIMKDRIAPA
                     QVRRGEMINRAAARLT"
     gene            complement(1211..1621)
                     /locus_tag="VLI90_08605"
     CDS             complement(1211..1621)
                     /locus_tag="VLI90_08605"
                     /inference="COORDINATES: protein motif:HMM:NF006040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HSV14307.1"
                     /translation="MKLFLLKFFTWWSGQTFGTQLWTWRFGELVGEDEQGNRYYRTKG
                     RKIDPTLGFERRWVVYNGYAEASRVPPSWHGWLHHTVDVAPTEENYTPREWEKPHVPN
                     MTGTAAAYRPSGSTLASGRRPKATGDYQAWTPGN"
     gene            1886..>2516
                     /locus_tag="VLI90_08610"
     CDS             1886..>2516
                     /locus_tag="VLI90_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14308.1"
                     /translation="MINLKVLSTVAAIALVVPMASAVQAQDRGGPAGVGGGGGARAGG
                     GGGAAIGGGGGGFRGGGGGGGPAIGGGGGGGFRAGASAPSMGGAPSGFRSAPTMGFRA
                     TPGSGPAPAVRGSEGNRFVAGGGTIRQGSSGNFAGRGQYAGGGYNGGYRHRHRGGGFW
                     PGVAVGVGVGSTYGYYGSPYYDDSYGYYDDTTVAAGPPAGDDSVAYCMQR"
BASE COUNT          427 a          813 c          816 g          460 t
ORIGIN      
        1 atcatgttgc tctccgatct tagcttcagc ctcgactttc ggggcttgtc ctaaaatgag
       61 ggaaaaatgc catttcggac atcaagcgac catgtcagga tgccccccgt caagccaatt
      121 tttccggagg tcacgatgtc cgcgccgtct cccacgccca cgccactgca aatcggtctc
      181 gtgctgttcc caaaagtcac ccagctcgat ttcaccggtc cgctgcaggt gttctccagc
      241 ctgcccggcg ccaaggtgca tctgatctgg aaacacatcg aaccggtcac gagcgattcc
      301 gtgctggtgc tgacgccgac cacgaccttt gcggactgcc cgcagctcga cgtcatctgc
      361 gtgcccggcg ggttcggcac cgacgacatg atcaacgacg aggagatgct ggccttcctg
      421 cgcaagcagg cggcgggcgc gaaatacatc acctcggtct gcacgggttc gctggtgctc
      481 ggcgccgccg gcctgctcaa aggctatcgc gcggcgacgc actggaccgc gatggatttc
      541 ctctccgcgt tcggcgcgat cccgaccagg acacgggtct gcgtcgaccg caaccgcgtc
      601 accggcggcg gcgtcaccgc cggcatcgac ttcgcgctga cgctggtctc gctgctgcac
      661 gaccgaaaga ccgcggaggc gatccagctc cgcctcgaat acaatcccgc gccgccgttc
      721 aattccggct cgccggacac cgcaccgccg gaagtgctcg ccatcatgaa agacaggatc
      781 gcaccggcgc aggtccgccg cggcgaaatg atcaaccgcg ctgctgcacg actgacatga
      841 atctgtgtcc cggacacgca agccggacac caagaaaaag gccgcctggt tgcccagacg
      901 gcctttcctt tctcacggta gccacacatt ggcgggcgat ttcagtccga ctgcaccggg
      961 ggcctatttc ccagccgtgt cctgatcggt ggccgctgct agccggggca gtcgcgaact
     1021 ggagcccgac accgacgcga tgggtttgcc atctccgtaa gatgccccca ttgagccgcg
     1081 agaacgcgga tggcgcaaga cggcgagcgc gaatgtcccc gctgatcccg ttattcacaa
     1141 cagcgccagt tgggctttca cgcgcgtgca catgcacacg ctcacggagg gcttgggtgt
     1201 acgagccgtc tcagtttccc ggcgtccagg cctgataatc gccggtggcc ttggggcggc
     1261 gcccgctggc cagcgtcgag ccggaggggc gataggccgc agccgtgccg gtcatgttcg
     1321 gcacatgcgg cttttcccat tcccgcggcg tgtagttctc ttcggtcggg gcgacatcga
     1381 cggtgtgatg cagccagccg tgccaggacg gcggcacacg ggatgcttcg gcatagccat
     1441 tatagaccac ccagcgccgc tcgaagccga gcgtcggatc gatcttgcgg cccttggtgc
     1501 ggtagtagcg gttgccctgc tcgtcttcgc cgaccagttc gccgaatcgc caggtccaca
     1561 attgcgtgcc gaaggtctgg ccgctccacc aggtgaaaaa tttcagcaga aacagcttca
     1621 tgagggctcg gccggttcga gatgtcgtgc cgctctgatg ccatcggcgg gccggattgt
     1681 ccagacaaag gctgatgttg caagcccgcc ggactggcct gttgcggccg tttgtcccac
     1741 gcagaaccgc ggaatcggca caatccgttc attccgcgta cagttccgac ctgacaggct
     1801 cagctcgcgc gcaatcaacg gcgggtacgg gaacgtccgc ctgttgaacg ggttaggaac
     1861 cgatatcgcg caaatggagc caacaatgat caatcttaaa gttttgagta cggtcgcggc
     1921 gatcgcgctc gtggtgccga tggccagcgc cgtgcaggcg caggatcgcg gcggcccggc
     1981 aggcgtgggt ggtggcggcg gagccagagc cggtggcggt ggcggcgcag ctattggcgg
     2041 cggtggcggt ggcttcaggg gcggcggcgg tggcggcggt ccagctatcg gcggtggcgg
     2101 aggcggcggc ttcagggccg gtgccagcgc gccttcgatg ggcggcgcac cttccggatt
     2161 ccgatccgcc cccacgatgg gattccgtgc cacgcctggc agcggtccgg caccggctgt
     2221 tcgtggcagc gaaggaaatc gcttcgtggc cggtggcggt acgatccgcc aaggttccag
     2281 cggcaacttt gctggtcgcg gccagtatgc tggcggcgga tacaacggag gctatcgtca
     2341 ccgtcaccgt ggtggcggct tctggccggg cgtcgcggtc ggtgtcggcg tagggtcgac
     2401 atatggctat tacggcagcc cctattacga cgactcctac ggatattatg acgacaccac
     2461 cgtcgccgcg ggacctccgg ctggcgatga ttcggtcgcg tactgcatgc agcggt
//
LOCUS       DATIMH010000492         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4740454, whole genome shotgun sequence.
ACCESSION   DATIMH010000492 DATIMH010000000
VERSION     DATIMH010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4740454"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1141)
                     /locus_tag="VLI90_08615"
     CDS             complement(92..1141)
                     /locus_tag="VLI90_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV14309.1"
                     /translation="MNSLSVGIIGYGRIGHEHAAWLRQSAAARPAAVADTTPGRRALA
                     ASSGLRVYEQIDPMLRDASIDAVLIATPTSMHFDHAMAALAADKHVMIEKPMALDLPQ
                     GKRIVEEAGRRNRIVSVFHNRRWDADYLTVRSAISSGLIGKIINVESRLGQWSSCVGP
                     AAKEYRPGWRNEAAFGGGGLYDWGSHFIDQLWRLLWPARPMRVFAQLRGNVWTSDCDD
                     FARVCIDFDNGAAGLLEINTTTTRPLPRWHIDGSAGSAESPFSLRFEVSTWAQLTFTP
                     SAAGSASQLLPVAKSGLDEAQIWDRFAAAARGEGQPAVTVASVLPTMALLDAARESAR
                     TGTVVALPLDLAWVY"
     gene            complement(1372..4218)
                     /locus_tag="VLI90_08620"
     CDS             complement(1372..4218)
                     /locus_tag="VLI90_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HSV14310.1"
                     /translation="MIQAIVQPLVLQAAPTTTTPTTMTAAPATSFGGFSDATIGPKLS
                     ASTNRLLFSNVIGATASPASVLTIINTGTADLTISALTLGGADGAMFTVSTNPGTIPA
                     GTSVTLSLKFAPPTGATAGIKLGTLEIDSNDANTPATIINLRGLAAAGVGGSLEPSLQ
                     RILDLYQIPDNVGDSNPDDTFIDMPAVQPNDEVPIQRLRRAAAGPVTITPLAVYALKV
                     APALRFGYYTPGYTTNNRTELFTVGSADAQTVNPHFLGATSFDPGFTPFSLYTTWPGF
                     KNNDGQIRTSYSENSLNYWETNPIAQHKIRFYPMKNADGSIVANTYIFAVEDFNAGYD
                     SNDLVGVISNVKRAARMANPAMGLSAGNGLLFNDRLVFSKAQFFNPNFPDPVYHDTET
                     LTIRNTGANALNISSMSIAGAFAIVAGPTGATSVDPGASITLTIKFTGSSGGTLTGQR
                     YDGLLTILSNDPTSPKMTVQLAGQWQSYPNSTPSPEYYTEPTAMQLTSLFGYSTQIVY
                     SGQVLNTGGSKTAVGEEVLSDYWSRADATQPVSVRELAAFHTGDTTSTIRWFSKGSTG
                     LNGIFTHLATDAQTILPRMNTNGALAQGSFSPSGAFAFKIDNEYSVDSMNPQDPITGN
                     QGHDVRIYPARDRANRYIPNTWLLYMDYYGMNYDYQDNIYLIQNMKPSAAPAAPADLA
                     ASGTNANGILLQWAANPETNIVGYNIYRSTVINSGYTLINSGPVTQTTYLDDVPLGTY
                     FYRVTAVNALNIESLPAQATTLRPQMWYTGMDIGGPTIAGHTTQLVAGSDFDIYGGGS
                     DISGRNDHFHYAFGWQYGDFDVQVRITSLDNTDPLAKAGLMVRAGLTSYSRQLLIGAT
                     PSGFVGIARQTDDAVATVANGGPTTYPNTYVRLLRVGASLTGFVSNDGVNWTQIVSMI
                     DPTLPDVVVMGMAVTGHSTTAGANASFRGLTSMP"
BASE COUNT          729 a         1331 c         1471 g          796 t
ORIGIN      
        1 gacgcctgcg tcgcctccgc gtgaagaggc gacgcaggcg tcgcagctat gggatttaag
       61 atgcgatccg ccggttgaat accggcgaag atcagtacac ccacgccaga tccaggggta
      121 gcgcgacgac cgttccggta cgcgcgcttt cgcgcgccgc atccagcagc gccatcgtcg
      181 gcagcacgct cgcgaccgtc accgcgggct ggccttcgcc gcgggcggcg gcggcgaaac
      241 gatcccagat ttgcgcctcg tctaatccgg acttggcaac cggcaacagc tgcgatgctg
      301 acccggcggc gctgggggtg aacgtcagct gcgcccaagt gctcacctca aaccgaaggc
      361 taaacggact ctccgccgac ccggcggagc cgtcgatgtg ccatcgcggc agcggccgcg
      421 tcgtcgtcgt gttgatctcg agcaacccgg cggcgccgtt gtcgaagtcg atgcagacgc
      481 gggcgaagtc gtcgcagtcg ctcgtccaca cgttgccgcg aagttgagcg aagacgcgca
      541 tggggcgcgc gggccagagc aatcgccaga gctggtctat aaagtgcgag ccccagtcgt
      601 acaacccacc gccaccgaag gcagcttcgt tgcgccaacc cgggcggtac tccttcgccg
      661 ccgggccgac gcagctggac cattggccga gccggctttc cacgttgatg atcttgccga
      721 tcagcccaga actgatcgcg ctgcgcacgg tgagatagtc ggcgtcccac cggcggttgt
      781 gaaacacgct gacgatgcga ttgcggcggc ccgcctcttc gacgatccgc ttgccctgcg
      841 gcaggtcgag cgccatcggc ttctcgatca tgacgtgttt gtcggcggcc agcgccgcca
      901 tcgcgtggtc gaagtgcatc gacgtgggcg tggcgatcag cacggcgtcg atgcttgcat
      961 cgcgcaacat cggatcgatc tgttcataca cgcgcaggcc cgatgaggcg gcgagcgcgc
     1021 gccggcctgg cgtggtgtcg gccacggcgg cggggcgcgc ggctgctgat tgccgcagcc
     1081 acgccgcgtg ttcgtggccg atccggccgt aaccgatgat gccgacggag agagaattca
     1141 ttcgagcatc atcgtacgct gatcttgctc ccgctcccac gtgcgcgtgg gaggaggcac
     1201 agcccggagt tcaattgaca acagttgcaa atcaccctca ccctgccctc tcgcggagta
     1261 ccgggcgagg ggactggaat ggcaactcta ctcctgtcag acaaaaagaa accaccgcaa
     1321 gccgaccatc ggcttgcggc ggttctgtcc actaaagagc tctacaagag atcacggcat
     1381 cgacgtcagc ccgcggaacg acgcgttcgc gccggccgtc gtgctgtggc cggtgacggc
     1441 catccccatc accaccacgt ccggcagcgt cgggtcgatc atcgagacga tctgcgtcca
     1501 gttcacgccg tcgttgctga cgaagccggt cagcgatgcc cccacgcgga gcagccggac
     1561 gtacgtgttg gggtacgtcg tcggtccgcc gttggccacc gtcgcgacgg catcgtccgt
     1621 ctgccgggcg atgccgacga agccgctcgg cgttgcgccg attaacagct ggcgcgagta
     1681 cgacgtcagg cccgctcgca ccatcaggcc ggctttggcc agcgggtcgg tgttgtcgag
     1741 cgacgtaatg cgcacctgca cgtcgaagtc gccgtactgc cagccgaagg cgtaatggaa
     1801 gtggtcgttg cgcccgctga tgtcggaccc gccgccgtag atgtcgaagt cgctgccggc
     1861 gaccaactgg gtcgtgtgac cggcgatggt tggaccgccg atgtccatcc cggtgtacca
     1921 catctgcggg cgaagcgtcg tcgcctgcgc cggcagcgac tcgatgttca gcgcgttcac
     1981 cgccgtcacg cgatagaagt acgtgccgag cggcacatca tccaggtagg tcgtctgcgt
     2041 caccggtccg ctgttgatca gggtgtagcc gctgttgatc acggtgctgc gatagatgtt
     2101 gtaaccaacg atgttggtct cgggattggc ggcccattgc agcaggatgc cattggcgtt
     2161 ggtgcccgag gccgccaggt ccgccggcgc cgcgggggcc gccgacggtt tcatattctg
     2221 gatcaggtag atgttgtcct gatagtcata gttcatcccg tagtagtcca tgtacagcag
     2281 ccaggtgttg gggatgtagc ggttggcgcg atcgcgcgcg ggatagatgc gcacgtcgtg
     2341 gccctggttg ccggtgatcg gatcctgcgg gttcatcgaa tcgacgctgt attcattgtc
     2401 gatcttgaac gcgaaggcgc cggatgggct gaacgagccc tgggccagcg ctccattggt
     2461 gttcatgcgc ggcaggatcg tctgcgcgtc ggtggccaga tgcgtgaaga tgccgttcag
     2521 gcccgtgctg cccttgctga accagcggat ggtggaagtg gtatcgccgg tgtggaacgc
     2581 cgccaactcg cgaacggaca cgggctgcgt cgcatcggcg cgcgaccagt aatcgctgag
     2641 cacttcttca cccaccgccg tcttcgagcc gccggtgttc agcacctgcc cgctgtaaac
     2701 gatctgcgtc gagtaaccaa acagcgacgt caactgcatt gcggtcggct cggtgtagta
     2761 ctcgggcgac ggcgtggaat tcggataact ctgccactgc ccggccaact gcacggtcat
     2821 cttcggcgac gtcgggtcgt tcgacaggat cgtcaacaac ccgtcgtagc gctgccccgt
     2881 cagcgtgccg ccgctgctgc cggtgaactt aatcgtcagc gtgatcgacg cgcccggatc
     2941 gaccgacgtc gcgccggtcg gaccggcgac gatcgcgaat gccccggcga tgctcatgct
     3001 gctgatgttc agcgcgttcg cgccggtgtt gcggatcgtc agcgtttcgg tgtcgtgata
     3061 caccgggtcg gggaagttcg gattgaagaa ctgtgccttg gagaagacca gacgatcgtt
     3121 gaacagcaaa ccgttgccgg ccgacaggcc catcgcggga ttcgccatgc gggcggcgcg
     3181 cttcacgttg ctgatgacgc caaccaagtc gttgctgtcg tagccggcgt tgaagtcttc
     3241 gacggcgaag atgtacgtgt tggcgacgat cgaaccatcg gcgttcttca tcggatagaa
     3301 gcggatcttg tgttgggcga tcgggttggt ttcccagtag ttcagcgaat tttcgctgta
     3361 gctggtgcgg atctggccgt cgttgttctt gaagccgggc caggtcgtgt acagcgagaa
     3421 cggcgtgaag ccggggtcga agctggtcgc gcccaggaag tgcgggttga ccgtctgcgc
     3481 gtcggccgag ccgacggtga acagctccgt gcgattgttg gtcgtgtagc cgggcgtgta
     3541 gtaaccgaaa cgaagcgccg gcgcgacctt caacgcatac actgccaacg gcgtgatcgt
     3601 caccggcccg gcagcggcgc ggcgaaggcg ttggatcggc acctcatcgt tcggctgcac
     3661 cgccggcatg tcgatgaacg tgtcgtcggg gttggagtcg ccgacgttgt cggggatctg
     3721 atacagatcg aggatgcgct gcagcgacgg ttcgagcgaa ccgccgacgc ccgcggcggc
     3781 gaggccgcgc aggttgatga tcgtcgccgg cgtgttggcg tcgttgctgt cgatctcgag
     3841 cgtccccagc ttgatgccgg cggtggcgcc agtcgggggc gcgaatttca agctcaaggt
     3901 gaccgacgtg ccggcgggga tcgtgccggg gttggtcgag acggtgaaca tcgccccgtc
     3961 ggcaccgccg agcgtcagcg cgctgatcgt gaggtcggcg gtgccggtgt tgatgatcgt
     4021 cagcacgctc gcggggctgg cggtagcgcc gatcacgttg ctgaacagca agcggttcgt
     4081 gctggccgac agctttggcc cgatcgtcgc gtcgctgaac ccgccgaacg aagtcgccgg
     4141 ggcggcggtc atcgtcgtcg gggtcgtcgt cgtcggggca gcttgcagaa ccaggggttg
     4201 aacgatcgct tgaatcacgt tggtggctcc acgcgaaaag gaacgggaac aaccgccgaa
     4261 tccatcgact ccgatggacg ggcgtgagcc aacaatgcca ccgattcggc gcgcggcgag
     4321 cggatga
//
LOCUS       DATIMH010000493         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_117394, whole genome shotgun sequence.
ACCESSION   DATIMH010000493 DATIMH010000000
VERSION     DATIMH010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117394"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VLI90_08625"
     CDS             <1..413
                     /locus_tag="VLI90_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HSV14311.1"
                     /translation="LSVPRVTDWKHLDLNYKIVAHIDPAKCIGCELCYTACWDGAHQC
                     IHIEGHKYPESLEAASRARIAVTPIAKLDALQAANGKKTPPARIPTVDEAECVGCNLC
                     WLVCPVEQCITMERVETGKPRQSWEQRTAAASKG"
     gene            418..1812
                     /gene="hydA"
                     /locus_tag="VLI90_08630"
     CDS             418..1812
                     /gene="hydA"
                     /locus_tag="VLI90_08630"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HSV14312.1"
                     /translation="MPNTLIRNGTVVTARDTKAADVLIEGGRIAEVRAGIPADRAENI
                     IDAAGMYVIPGGIDAHTHLDMPFGGTTSADDFETGTRAAAFGGTTSIVDFAIQARGTK
                     MRDALDTWWKKAESKAVIDYGLHMIVTDLGTSGLEDMDELVDEGVASFKLFMAYPNVL
                     MVDDATIFKALSRTAKNGALICMHAENGSVIDVIIAKALAEGKTAPIYHALTRPTRAE
                     AEAVHRAIAMAEIAGAPVYIVHLSSEDALNEVREARDRGVPAFAETCPQYLLLSIEEL
                     ERPNFEGAKYVFTPPLRTKENLPKLWDGLKHDHLQVVSTDHCPFCFEDQKILGKDDFT
                     KIPNGGPGIENRLQLIYHHGVNAGEISLNRFVEITSTTPARIFGMYPRKGEIAPGSDA
                     DIVIWDPKAPFTISAKTHHMRVDYSMFEGYQVQGNARTVISRGEVIVDGGQFYGKPGR
                     GEYLRRQARGGAWK"
     gene            1830..3215
                     /locus_tag="VLI90_08635"
     CDS             1830..3215
                     /locus_tag="VLI90_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HSV14313.1"
                     /translation="MDLAPIPMERRTWGTYNYASLWVAMSVCIPTYMLASGLIAGGMS
                     WWQAIATILLGNLIVLVPMLLNAHAGTKYGIPFPVLVRASFGVRGANVPAVLRALVAC
                     GWFGIQTWIGGQAIYSMLKIMWPGAATLPGGIWICFFAFWALNIAVIWRGIETIKFLE
                     GIGAPFMLGIGLLLLWWITSKAGGFGPVLSAPSKFHTAAEFFRFFIPSLTGMVGFWAT
                     VALNIPDFTRYAKSQKAQAMGQALGLPIAMTLYSFIGVAVTSASVVLFGEAIWDPVTL
                     LGKFNQPVVAFIALIALLIATLNTNVAANVVSPSNDFSNLNPRLISFRTGGLITGVIG
                     VLMMPWKLLTDFSSYIFGWLVGYSGLLGPIAGVMIVDYFVVRRSRLRVEDLYRRDGIY
                     AYENGVNLRAIAALAIGIAVALLGLFVPALRLLYDYAWFVGFGVSGAVYRLLMQGAAV
                     PANVPEWEGEA"
BASE COUNT          600 a          992 c         1071 g          622 t
ORIGIN      
        1 gcctcagtgt cccccgcgtc acggattgga agcatctcga cctgaattac aagattgtcg
       61 cgcacatcga tcccgccaaa tgcatcggct gcgagctgtg ctacaccgcc tgctgggacg
      121 gtgcgcacca gtgcatccac atcgaaggcc acaaatatcc cgagagcctc gaggcggcca
      181 gtcgcgcgcg catcgccgtg accccgattg ccaagctgga cgccctgcaa gccgccaacg
      241 gcaagaagac accgccggcg cgcatcccca cggtggacga agccgagtgc gtgggctgca
      301 atctctgctg gctggtctgt ccggtggagc aatgcatcac catggaacgg gtggagacgg
      361 gcaagcccag gcaatcgtgg gaacagcgta cggcggcggc atccaaaggt tgagatcatg
      421 ccaaacactc tgattcggaa tggcactgta gtaaccgcac gcgatacgaa agcggcggac
      481 gtattgatcg aaggtgggcg catcgctgaa gtgcgcgccg gaatccccgc ggatcgggcg
      541 gagaacatta tcgacgcggc cggtatgtac gtgatacccg gcggaatcga cgcgcacacg
      601 cacctcgaca tgccgttcgg cggcaccacc agcgccgacg atttcgagac gggaacgcgc
      661 gcagccgcgt tcggcggcac caccagcatc gtggatttcg ccattcaggc gcgcggcacg
      721 aagatgcgcg acgccctgga cacctggtgg aaaaaggccg agagcaaggc ggtgatcgac
      781 tacggcctcc acatgatcgt caccgacctg ggcacgtccg ggctggagga catggacgaa
      841 ttggtggacg aaggcgtcgc cagtttcaaa ctgttcatgg cgtatccgaa cgtcctgatg
      901 gtggacgacg cgacgatctt caaagcgctc tcacgcaccg cgaaaaatgg cgcgctcatc
      961 tgcatgcacg ccgagaacgg cagcgtgatc gacgtgatca tcgccaaggc gctggccgaa
     1021 gggaagacgg cgcccatcta tcacgcccta acgcggccga cgcgcgccga ggccgaggcg
     1081 gtgcaccgcg ccatcgccat ggcggagatc gccggggcgc cggtctacat cgtgcacctt
     1141 tccagcgagg acgcgctcaa cgaagtccgc gaggcgcgcg accgcggcgt gccggcattc
     1201 gccgaaacgt gcccgcaata cctgctgctg tcgatcgagg aactcgagcg gccgaatttc
     1261 gaaggcgcga agtacgtgtt cacgccgccg ctgcgcacca aagagaactt gcccaagctc
     1321 tgggacgggc tgaagcacga tcatctgcag gtcgtttcca cggaccactg tccgttctgc
     1381 ttcgaggacc agaagattct cggcaaggac gatttcacca agattcccaa cggcggcccg
     1441 ggcatcgaga atcgcctgca gttgatctat caccacggcg tgaatgcggg cgaaatttcg
     1501 ctgaaccggt ttgtcgaaat cacctcgacc acgccggcgc gcatcttcgg catgtatccg
     1561 cgcaagggcg agatcgctcc gggcagcgac gccgacatcg tgatctggga tcccaaggcg
     1621 ccgttcacca tcagcgccaa gacgcatcac atgcgcgtcg attactcgat gttcgaaggc
     1681 taccaggtgc agggcaacgc tcgtaccgtg atctcgcgcg gcgaggtgat cgtcgacggg
     1741 ggccagttct acggtaagcc cggccgcggc gagtatctgc gtcgacaggc ccgcggagga
     1801 gcatggaagt agaccccact ctgtataacg tggacctggc gccgatccct atggagcggc
     1861 gcacctgggg cacgtacaac tacgcttcgc tgtgggtggc catgtcggtc tgcattccga
     1921 cttacatgct ggcgtccggc ctgattgccg gcggcatgag ttggtggcag gctatcgcga
     1981 ccatcctgct gggcaatctg attgtgctcg tcccgatgct gctgaatgcg catgccggca
     2041 cgaagtacgg cattcccttc cccgtgctgg tgcgcgcgtc gttcggcgtg cgaggggcga
     2101 atgtgccggc agtgctgcgc gcgctggtcg cctgcggctg gttcggcatc cagacctgga
     2161 tcggcggaca ggccatctac tcgatgctca agatcatgtg gccgggcgcg gcgaccctgc
     2221 cgggcggcat ctggatctgc ttcttcgcgt tttgggcgct caacatagcg gtgatctggc
     2281 gcggcatcga gaccatcaag ttcctcgaag ggatcggcgc gccgttcatg ctgggcatcg
     2341 ggctgctgct gctctggtgg atcaccagca aggccggcgg cttcggtccg gtgctgagcg
     2401 cgcccagcaa attccacacg gccgccgagt tcttccgctt ctttattccg tcgctgaccg
     2461 gcatggtggg gttctgggcg acggtcgcgt tgaacatccc ggacttcact cgctacgcca
     2521 aatcgcagaa ggcgcaggcc atgggacagg ccctcggact gccgatcgcg atgacgctgt
     2581 actcgtttat cggcgtggcc gttacgtcgg cgtcggtggt gctgttcggc gaggcgatct
     2641 gggatccggt gacgctgctg ggtaagttca accagccggt ggtggcgttc atcgcgctga
     2701 tcgcgctgct catcgcgacg ctgaatacga atgtggcggc gaacgtcgta tcgccgtcga
     2761 acgatttctc gaatctgaat ccgcggttga tttcgtttcg taccggcggc ctgattaccg
     2821 gcgtgatcgg cgtgctcatg atgccgtgga aactgctgac cgatttcagt tcgtatattt
     2881 tcggctggct ggtggggtac tcgggactgc tggggccgat cgccggcgtg atgatcgtcg
     2941 actatttcgt ggtgcggcgc tcgcggttgc gggtcgagga cctctaccgg cgcgacggca
     3001 tctacgctta tgaaaatggc gttaacttgc gggctatcgc cgcactggcg atcggcatcg
     3061 cggtcgcgct gctgggactc tttgttcccg ccctgcgact gctgtacgac tatgcctggt
     3121 ttgtcggctt cggagtctcc ggggctgtgt atcgtttgct gatgcagggg gcggctgtcc
     3181 ctgccaacgt tccggaatgg gaaggtgaag catgattgct acaggaaatg cagtgggaac
     3241 cgtatcgttc tacgtcaacg gcgtttggga gaagctcgac gggcg
//
LOCUS       DATIMH010000494         3318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5318684, whole genome shotgun sequence.
ACCESSION   DATIMH010000494 DATIMH010000000
VERSION     DATIMH010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5318684"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VLI90_08640"
     CDS             <1..284
                     /locus_tag="VLI90_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSV14314.1"
                     /translation="GEIHDFRPGAIGRAMALLARNRFVIGGVIAMAVSFFAYMGLLTI
                     ADLSFAVPATAVTYVLETILAKHVLKEHVNGLRWAGAALVVCGVVLVSL"
     gene            281..1405
                     /gene="hpnI"
                     /locus_tag="VLI90_08645"
     CDS             281..1405
                     /gene="hpnI"
                     /locus_tag="VLI90_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HSV14315.1"
                     /translation="MTFWAAPALAGAAYYVLVLAGAVRWFLGDRDPKRCAGTSGRLTE
                     PISILKPIRGRDPKFFEAIRSHASQDYPEFEILFGLSDPKDAAAKDIARLQAEFPRLP
                     ISAYVISTKAPNAKVGVLAELARRAAHPVLLVNDSDIVVEPGYLRAVVAPLADTRVGL
                     VTCLYRAAAESWPSRSEALGIATEFAPSVIVARLLGVAEFALGSTMVFRADSLRAIGG
                     FEALQNYLADDYQLGRHITEELRLRIEFAPVIVETDLGAESWLEVWRHQLRWSRTIRV
                     SRRSGYYGYVVTHATLWALMAFAARQWWAGAVALGVRMLAGVVVGRGILGYRRVLRDC
                     WMIPLRDLFGFAVWLGGLFGDEVLWRGKRLSLGHDGRIRL"
     gene            complement(1334..3076)
                     /locus_tag="VLI90_08650"
     CDS             complement(1334..3076)
                     /locus_tag="VLI90_08650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV14316.1"
                     /translation="MHVVVIPAYRPGSTLPRLIADLTRLSAAPIVVVDDGSGVDFEAT
                     FARAAAHKDVHVVRHCTNLGKGAALKTGLNYAMVEFPCCSGVVTADADGQHVAEDIIA
                     VGERMEAEPKKLVLGVRSFDESVPLRSKIGNGATRILMRLVVGQKLADTQTGLRGIPA
                     DLIPLLLRLPSSGYEFELDMLILCKHQSCAIVEEPIRTVYIEGNQSSHFRPVADSMRI
                     YFLLFRFGLLSLLTAALDNLVFIGAFSITGSIGRSQIAGRAVAMVFNYLGAHRAVFHS
                     KQKHAAVIPRYVAVVIANGLASYALIQLLHFRFGIATVPAKLAAEGLLFLANFAIQRD
                     LVFTKRPESPTATNWDAYYTSVPPTAKLTRRYSTGVLLDILRRHAVANNGEPLSIVEI
                     GGANSCFMQEILRAIPCRSYDVVDTNEYGLSLLESQVREGSPVRLHCESVLDLDWRAQ
                     ADVVFSVGLVEHFDPARTRQAILAHFSVLRPGGTAIITYPTPTLLYRVTRGLIEAAGM
                     WKFHDERPLKPAEVASSITEQADILQQKIMWPLMLTQHVVVARKRAAGSLQPDATVVT
                     QRKPFTAPENLIAE"
BASE COUNT          576 a          961 c         1134 g          647 t
ORIGIN      
        1 acggcgagat ccacgacttc cgcccggggg cgatcggccg cgccatggcg ctgctggcgc
       61 gcaatcgctt tgtaataggt ggagtgatcg cgatggcggt gtcgtttttc gcgtacatgg
      121 gacttctgac catcgccgat ttaagcttcg ccgtgccggc gaccgcggtg acctacgttt
      181 tggaaacgat cctggcaaag cacgttctca aagagcacgt caacggactg cgctgggcag
      241 gcgccgcact ggtcgtctgc ggcgtggtgc tggtctcgtt gtgacctttt gggcggcgcc
      301 ggcgctggct ggggcggcct attatgtcct cgttctggct ggggcggtgc gctggttttt
      361 aggtgaccgc gatccaaaac gctgcgccgg aacgtccggc cgcctgaccg aacccatttc
      421 catcctcaag ccgattcgcg ggcgcgaccc caagtttttc gaggccatcc gctcgcatgc
      481 gtcgcaggac tacccggagt tcgagattct cttcggactc agcgacccga aggacgcggc
      541 ggcgaaagat attgcgcgcc ttcaggccga attcccgcga ctgccgatct cggcgtacgt
      601 tatctcgacg aaagcgccga atgccaaagt cggcgtgttg gcggagttgg ccagacgcgc
      661 ggcgcatccg gtcctgctcg tcaatgacag cgacatcgtg gtcgagcccg ggtatctgcg
      721 ggccgtcgta gcgccgctcg ccgatacgcg ggtgggcctg gtgacgtgct tgtaccgcgc
      781 ggcggcggag tcgtggccat cgcggtcgga agcgctcggg atcgcaactg agttcgcgcc
      841 gagtgtgatc gtcgcgcgct tgctgggagt ggcggagttc gcgttagggt cgacgatggt
      901 gtttcgggct gactcgctgc gggccatcgg aggctttgag gcgttgcaga actaccttgc
      961 ggacgactac cagctcggcc ggcacattac agaggagctc cgattgcgaa ttgaattcgc
     1021 gcccgtgatc gtggagacgg acctgggagc ggagtcatgg ctggaggtgt ggcggcatca
     1081 attgcggtgg tcgcgcacga tccgcgtttc gcggcgctcg ggatactacg ggtatgtggt
     1141 cacgcacgca acgttgtggg cgctgatggc gtttgcggcg cggcaatggt gggcgggcgc
     1201 ggtggcgctc ggtgtgcgaa tgctggcggg cgtggtggtt gggcgcggca ttctggggta
     1261 ccggcgcgtg ctgcgggact gttggatgat tccgctgcgc gatcttttcg ggttcgcggt
     1321 ctggttgggc ggcttattcg gcgatgaggt tctctggcgc ggtaaacggc tttcgctggg
     1381 tcacgacggt cgcatccggc tgtagcgaac ccgccgcgcg ctttcttgcc acgacaacgt
     1441 gctgcgtcag catcagcggc cacatgatct tctgctgcag gatatcggcc tgctccgtga
     1501 tggacgaagc cacctcggcg ggcttgagcg gccgttcgtc atgaaacttc cacatccctg
     1561 ctgcctcgat gaggccgcgg gtgacacggt agagcagcgt cggggtcggg taagtgataa
     1621 tcgcagtgcc gccgggccgc agaacgctga agtgggccag aatcgcctgg cgcgtgcgcg
     1681 ccgggtcgaa gtgctcgaca agcccgacgc tgaatacgac atccgcctgc gcccgccagt
     1741 cgaggtcgag cacgctttca cagtgcagcc gcacgggcga tccctcgcgg acttgactct
     1801 ccagcagcga aagcccgtac tcgttggtgt cgacgacgtc gtacgagcgg cacgggatcg
     1861 cgcgcaggat ctcctgcatg aagcagctgt tggcgccgcc gatttcgacg atcgacagcg
     1921 gctcgccgtt attagcaacg gcgtggcgcc gcaggatgtc gagcagcact cccgtgctgt
     1981 accggcgggt cagcttcgca gtgggcggca cgctggtgta gtaggcgtcc cagttggttg
     2041 cggttggaga ctccgggcgc ttcgtgaaga ccagatcgcg ctggatcgcg aagttcgcca
     2101 ggaataacaa cccctcggct gccagtttcg ccggcacggt cgcgatgccg aagcggaagt
     2161 gcagcaattg aatcagggcg taggatgcca gtccattggc gataacgacg gcgacgtacc
     2221 ggggaatgac ggctgcgtgt ttttgcttgg aatggaagac cgcgcgatgg gctccgaggt
     2281 agttgaagac catggcgacc gcgcgccccg caatctggga ccggccgata cttccggtaa
     2341 tgctgaaagc accgatgaag accaggttgt cgagagctgc cgtgagaagg gagagcaggc
     2401 cgaaccggaa aagcaggaaa tagatccgca tggaatccgc cacagggcgg aaatgcgaac
     2461 tctggtttcc ctcgatgtag acggtgcgga tcggctcctc gacgatcgcg caggactggt
     2521 gcttgcacaa aatgagcatg tccagctcaa actcatagcc agaggacgga agccggagca
     2581 gcagcgggat gagatccgcc gggatgccgc gcagcccggt ttgcgtgtcg gcgagctttt
     2641 ggccgaccac caggcgcatc aggatgcgag tggcgccgtt cccaattttg ctgcgcagtg
     2701 ggacggattc atcgaagctt cgaacgccca aaacgagctt tttgggctcg gcttccatgc
     2761 gctcaccgac cgcgatgata tcctcggcga catgctgtcc atccgcgtcg gctgtgacca
     2821 ctccggaaca gcaggggaac tcgaccatcg catagttcag accggtcttc agtgctgcgc
     2881 ccttgccgag attcgtacaa tgccgaacga catggacgtc cttatgggcc gccgcgcgcg
     2941 cgaatgtcgc ctcaaaatcc acaccgctac cgtcgtccac gactacgatg ggggcggcag
     3001 acaggcgggt gaggtcggcg atcagccggg ggagcgttga accggggcga taggcgggaa
     3061 taaccacgac gtgcattgct ctactagtgt ataagacacc acccgcggct cgtgcgtgtt
     3121 ctgaaacgca gtattccgag aaacatgccc tttctcagcg ttgccttgct gcgtccaaac
     3181 gcgttaggct ccgggtgtgg tcatcctgct ctttggcccc cccggttgcg gcaaggggac
     3241 gcaagcagct tttctgatcg aacggctgcg cattccggcg atctcgactg gcgagatgtt
     3301 tcgggcagag tgcaaggc
//
LOCUS       DATIMH010000495         1732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3582886, whole genome shotgun sequence.
ACCESSION   DATIMH010000495 DATIMH010000000
VERSION     DATIMH010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3582886"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..550
                     /locus_tag="VLI90_08655"
                     /pseudo
     CDS             128..550
                     /locus_tag="VLI90_08655"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
     gene            721..1551
                     /gene="kduI"
                     /locus_tag="VLI90_08660"
     CDS             721..1551
                     /gene="kduI"
                     /locus_tag="VLI90_08660"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HSV14317.1"
                     /translation="MKVYLTPDPVRTASMNTEQLRESFLISNLFKPGQIDMVYCDVDR
                     AVVGSACPVAETLRLGTDDELRAAYFTERRELGILNIGGRGSVSVEGKTFEMDNLDGL
                     YIGRGNKDIRFTSADRNAPAEFYLLSYPAHTTHPTAHAKKAQAAAVNLGSEATCNKRT
                     IYKYIHLDGIKSCQLVMGFTQLQVGSDWNTMPPHTHLRRSEVYLYFNVPAEHRVFHMM
                     GQPKESRHLVVADKQAVISPPWSIHAGVGTSNYTFCWGMGGENQTFDDMDAAPIAMLR
                     "
BASE COUNT          367 a          533 c          503 g          329 t
ORIGIN      
        1 tcaagattac gaaaggattg tcgggatatc gcgcgctcca tggagaacgc gaatgacttc
       61 taccttctcc gcatcgattc tgtagaagac gaggtaattt tcaaagccga cgaccgctcg
      121 ctgccaggcc gtcggcagcg atgtccgcgt tcgtgtcaaa gagaccggca gctactgcta
      181 tccggatgtc gccgtggtgt gcggtcagcc aatctacgat ccgcctgacc gtgaaaccac
      241 catcgcgaac ccgcgagtta taatagaagt gagttcgccc tcaactgagg ctgatgaccg
      301 gggcaagaag ttcaatgact accgccggct tcaatcgctg caagagtatt ttctggtcgc
      361 gcaggatcgg cttagcgtcg aaaccttcta ccgtcagtcc gatggaatgt gggtcatcgg
      421 ccccaccctc acggaggtgc acgcgcgcgt ccaatttcgc tcgctcagcg tcgatgtttc
      481 gctcgccgac ctttacgccg gcgtcgagtt gccgcccgga cctcctatgg cggagcaagt
      541 cgcgagctga aatgatccga caaacgcgag ttgcccgacg ttcaactctc aaagctcgaa
      601 caacgggtgg ggcgcggctt agcccgtcga ttctgcggct ccgctcagac aacggcatcg
      661 ctggagatga catccccgcg cgcatttcgc tacactgaag gccaaggagc aatcgttgtc
      721 atgaaagtct acctcacgcc cgatcccgtg cgcaccgctt cgatgaacac cgaacaactg
      781 cgcgaatcat ttctcatcag caacctgttc aagccggggc agatcgacat ggtctactgc
      841 gatgtcgatc gcgccgtcgt cggctcggca tgtccggtgg ccgaaacgtt gaggctgggc
      901 actgatgacg agcttcgcgc agcgtacttc accgaacgcc gggagctggg catcctcaac
      961 atcggcggcc gcggcagcgt gagcgttgaa ggcaagacgt tcgagatgga caacctcgac
     1021 ggcctgtaca tcggccgggg gaacaaggac attcgcttca ccagcgccga tcgaaacgcg
     1081 ccggcggagt tttatctgtt gagttatccc gcccacacga cgcaccccac cgctcacgcg
     1141 aaaaaagcgc aggccgccgc ggtgaatctg ggctcggaag cgacgtgcaa caagcggacg
     1201 atctacaaat acatccacct cgacggcatc aagagctgcc aactggtgat ggggttcacg
     1261 cagctgcagg tgggcagcga ctggaacaca atgccgccac acacgcatct tcggcggtcg
     1321 gaagtctacc tgtacttcaa cgtgcccgcg gagcaccgcg tgtttcacat gatgggccaa
     1381 ccaaaggaat cgcggcacct ggtcgtcgcc gacaagcagg ccgtcatcag cccgccgtgg
     1441 tcgatccacg ccggcgtggg cacgagcaat tacacgttct gctggggcat gggcggggag
     1501 aaccagacgt ttgacgatat ggacgcggcg ccgattgcga tgttgaggtg atggtcagag
     1561 ccgcatctgt ccgtggcgtc cgatcaatta accaactttc tttgctggtt ctctccatcc
     1621 ctccggccgg cgcggaggta aggttactac cttcgcccca gcgtgctcgg cttctgcagt
     1681 gcgccgctga agatccgcaa agatcgcccg cagatcgaaa ccgaacttcg cg
//
LOCUS       DATIMH010000496         5241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4741368, whole genome shotgun sequence.
ACCESSION   DATIMH010000496 DATIMH010000000
VERSION     DATIMH010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4741368"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2859)
                     /locus_tag="VLI90_08665"
     CDS             complement(<1..2859)
                     /locus_tag="VLI90_08665"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSV14318.1"
                     /translation="MFAKKAAVTASARRRSSIRRAVVPAVLEALEERRLMSAALSLTN
                     LDGLPFNDRLIFNKVQNPDPKFPNVTHDTSSVKLTNTGDATLSVSALTVTGPWVFVNG
                     PTTFPLSIPVGGSTTLTLKFTQSTLPRHVGNQTNFTTNPNGGASITGSLVIQSNDPAH
                     VNTPVTLAGYWQSKSENNAEPNLTTIVNLVSGYTTNIGGSPDLPEPGNTPTRVGDEVA
                     SDFWQSANAAQPVVVRQLAGFHTQGNVVHTFWFTGGSTGIDSTVSHQLFSSKPLEGQS
                     LLPHSAAGTFANGSFTTSAPFGLRVDNEYSLNSLNNQAGGGHHFRFYPLLDRTGTVVP
                     NTWIVGMDYAVIQTENFDFQDNVYVVSNMMPAQVQTVVPPPTPTNFTATNAANPVLNW
                     TGVSYGKPFGYKVYRSTSASGPFTLISGASPIASSTTTFTDNASPPAGTTLFYRLTAV
                     DLASSSESVPATTTANAPAANTPVAVDDSFDTTTNATNVTEDLTANDSGLGVVRHITI
                     IGAPNHGGTVVPDTNDTGLVTYTPAAGFTGVETFTYQVSNGGTQSNTATVTFNVSQPV
                     VPSPALPIAGDQNFQVLAANTYTLTPTNVTDAEGNAITPASITIVTNPTRGTVTTNGG
                     TIVYTPDPTKTAGANFVGSDFFAYDVTDAQGHTSTNHGVITLNVGVEFGTNTGKRSLS
                     YTDATGNKPVATLNLGVADVFFNGNGQFTTDSHGRITVGSFSGASNLTIANVKLTNTT
                     AASTLAMVTPNRGAFSVGGIADSGTIGRIIAPNANVSGGSVANTTIDLGGDGILLLGQ
                     VSTSIINIGTGFTAGPAVVVGVVTDSLLNADGNVRLIKAVSWTNTENANLGIAAVSLN
                     TLLITGNATTAGDFEPNLSLSGNLNVASIAGTLDKGGWDINGNANVIVAKSVGAGWSG
                     VVSGTLNNMVVRTGGMPAPLTVTNLNTLNVLAGDITGGI"
     gene            complement(3271..>5241)
                     /locus_tag="VLI90_08670"
     CDS             complement(3271..>5241)
                     /locus_tag="VLI90_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSV14319.1"
                     /translation="GHYVWLEFTFRVLAGEGGGVREIHGSARDISQRMRIQQMEEDRA
                     EVLEMVVQNRPMVDVMSHLVKAAERLCPEAAAGCILLFDGELHHASGKLPPELQAALQ
                     ERIYSFTSRFCTDATMRGGDVFTSDVERDPVWEPVRRVALATGLQTCWASLIKSGHDD
                     VLGVFCLYHCSRVELDNSVASFLKMAGKLIMIAVEHHQLTDQLAYRAQHDALTGLPNR
                     VLFEDRLQQALLRAHISGKAVGVAFLDVDRFKNINDTLGHHAGDVMLCRLVARVLETL
                     GKTDTMARMGGDEFALILPDLSDAGDAQRFGERLIAAFKAPLDVLGQELFVTISCGLA
                     MYPKDGRDSITLKKNADAALYAAKNNGRDRMRCFTADMNNGTRERLDLENALRRAVEN
                     GELRLHYQPKVNADDQITGLEALLRWQHPTLGLVPPAKFIPLAEETGLIVNIGKWVLN
                     EAARQLQAWRRAGLPCVPVAVNVSAVQFAQPDFEQVLSAVLQMHALDPQWLELEITES
                     VLMQNMHDARQRLERLKQIGVSIAIDDFGTGYSSLVYLQRLPIDVLKVDRAFVSVIGS
                     GQPSGKGRDDATIVRSIAALAHSLGISLVAEGVETDYQRALLLDIGCPVMQGYLYSKP
                     RPPEEVEELLKAGRIARLVAPAPAPARVRLAS"
BASE COUNT          888 a         1642 c         1733 g          978 t
ORIGIN      
        1 gattccaccg gtgatgtcac cggccagcac gttcagcgtg ttcaggttgg tcacggtcaa
       61 cggcgccggc atgccgccgg tgcggacgac catgttgttg agcgtcccgg aaacgacacc
      121 gctccaaccg gcgccgacag acttggcgac gatcacgttg gcgttgccgt tgatgtccca
      181 gccgcccttg tccagcgtgc cggcgatcga cgcaacattc aggttcccgc tcaatgacag
      241 gttgggctcg aagtcaccgg cggtggtcgc gttgccggtg atcagcagcg tgttcagcga
      301 caccgccgcg atgccgaggt tggcgttttc ggtgttggtc caggagaccg ccttgataag
      361 ccgcacgttc ccgtcggcgt tcaagagcga atcggtgaca acgcccacca cgacggcggg
      421 accggcggtg aaaccggtgc cgatgttgat gatcgatgtg ctgacctgcc cgagcagcag
      481 gatgccatcg ccaccgagat cgatggtggt gttggcgacg gatccgccgc tgacgttggc
      541 gttgggggcg atgatccggc cgatcgtacc cgagtcggcg atgccaccga ccgagaacgc
      601 cccgcggttg ggcgtcacca tcgccagcgt gctggcggcg gtcgtgttcg tcagcttgac
      661 gttggcaatc gtcaggttgc tggcgccgct gaacgaaccg acggtgattc gaccatgcga
      721 atcggtggtg aactgaccgt tgccgttgaa gaacacatcg gcgacgccga ggttcaacgt
      781 cgcgaccggc ttattgccgg tggcatcggt atagctcagc gagcgcttgc cggtgttggt
      841 gccgaactcg acgccgacgt tcagcgtgat cacgccgtgg ttggtcgagg tgtgaccctg
      901 ggcgtcggtg acatcgtacg cgaagaagtc ggaaccgacg aagttggcgc cggcggtctt
      961 ggtcgggtcg ggcgtgtaca cgatcgtgcc gccgttggtg gtgacggtcc cgcgggtcgg
     1021 gttggtgacg atcgtgatgc tcgccggggt aatcgcgttg ccttcggcat cggtcacatt
     1081 ggtcggcgtg agcgtgtacg tgttggccgc cagcacttgg aagttctggt caccggcgat
     1141 cggcagtgcc ggcgacggca cgactggctg cgagacgttg aacgtcaccg tggcggtgtt
     1201 cgactgcgtc ccgccgttgg agacttgata ggtgaaggtc tcaacgccgg tgaagccagc
     1261 ggccggcgtg taggtgacca ggccggtgtc gttggtgtcg ggcactacag tgccgccgtg
     1321 gttgggcgcg ccgatgatcg tgatgtggcg aaccacaccc agcccgctgt cgttcgcggt
     1381 caaatcctcg gtcacgttgg tggcattggt ggtcgtgtcg aatgaatcat ccaccgcgac
     1441 cggcgtgttg gccgcggggg cgttggcggt cgtcgtggcg ggaaccgatt cactgctgct
     1501 ggccagatcg accgcggtca gacggtagaa cagcgtcgtc cccgccgggg ggctggcgtt
     1561 gtcggtgaag gtcgtcgtgc tggaagcgat cggcgaggcg ccgctgatca gcgtgaacgg
     1621 cccgctggcg gacgtgctgc gatacacctt gtaaccaaac ggtttgccgt acgaaacgcc
     1681 ggtccagttc agcaccggat tggccgcgtt ggtggcggtg aagttggtcg gcgtgggcgg
     1741 tgggacgacc gtctgcacct gcgccggcat catattgctg acgacgtaga cgttgtcctg
     1801 gaagtcaaag ttttcggtct ggatgaccgc gtagtccatg ccgacgatcc aggtgttggg
     1861 caccacggtg ccggtgcgat ccagcagcgg atagaaccgg aagtggtgtc ctccacccgc
     1921 ctggttgttc agggagttca ggctgtattc gttatcgacg cgcagcccga acggcgcgct
     1981 ggtggtgaag ctgccgttgg caaacgtgcc ggcggcgctg tgcggcagca gcgactggcc
     2041 ttccagcggc ttggagctga acagctggtg cgagacggtg ctgtcgatgc cggtcgaacc
     2101 accggtgaac cagaacgtgt gaacgacgtt accctgcgtg tggaagccgg ccagttggcg
     2161 gacgaccacc ggctgagccg cgttggcgga ctgccagaag tccgaggcca cttcgtcacc
     2221 gacgcgcgtc ggggtgttgc caggttccgg caggtccggc gagccgccga tgttcgtcgt
     2281 atagccggac accaggttga cgatcgtcgt cagattcggt tcggcgttgt tctccgactt
     2341 gctctgccag taaccggcga gcgtaaccgg cgtgttcacg tgcgccgggt cgttgctctg
     2401 gatgaccagc gagccggtga tcgacgcccc gccattgggg ttggtcgtga agttggtctg
     2461 gtttcccacg tgccgcggga gcgtgctttg ggtgaacttc agcgtcagcg tggtcgagcc
     2521 accaacgggg atcgacagcg ggaacgtcgt cggtccgttc acgaagaccc aggggccggt
     2581 cacggtcagc gcgctgacgc tcagcgtggc gtcaccggtg ttggtgagct tcacgctcga
     2641 ggtgtcatgc gtgacgttgg ggaacttcgg atcggggttc tgcaccttat tgaagatcaa
     2701 ccgatcgttg aacggcagac cgtccaggtt cgtcagcgac agcgccgccg acatcagccg
     2761 gcgctcttcc agcgcttcaa gcacggcggg gacaacagcg cggcggatgg aactgcgacg
     2821 gcgggcggat gcggtaacgg cggctttctt cgcgaacatg tggatcctcc aggatcggtc
     2881 taggagagcg gaacgtaacg actttcggga cgccggcgtc gatttattgc gacgccatta
     2941 gaaacaacaa actcaacgcg ggtggcacac aatcgaggag ctctgccaaa cacacacgga
     3001 tggtgccatg aggtgcacgc agccgattga cgccgccaag gcgaacttta tttcgctcgg
     3061 cccgctccgt cagcgctcaa tttcgcgcaa tcgcgtcgaa atgtcgcgtg ggggttacat
     3121 cggctcaggg gggcgggata gcttgagcaa cttcgcctac gaaatggctg caattgtctg
     3181 cgtgaaatag ggatgcgacg acgcggcgag cgacagcaat gcgacgtaag tttttatcag
     3241 acgtgcccac ggatcgcagc gatcgatcat ttacgatgcc agccgcaccc gcgccggcgc
     3301 cggcgccggc gcgaccagtc gcgcgatcct ccccgccttc agcagctctt cgacttcctc
     3361 cggcgggcgc ggcttgctgt atagatagcc ctgcatcacc gggcagccga tgtccagcaa
     3421 caacgcccgc tggtaatccg tttccacgcc ctcggccacc aggctgatac ccaggctgtg
     3481 cgccagcgcc gcgatcgaac gcacgatcgt ggcatcgtcg cggcccttgc ccgacggctg
     3541 gcccgatccg atcaccgaca cgaacgcccg gtccaccttc agcacgtcga tcggcaggcg
     3601 ctgcaggtac accagcgatg aatacccggt gccgaagtcg tcgatcgcga tgctcacgcc
     3661 gatctgcttc aaccgctcca gccgctgccg ggcgtcgtgc atgttctgca tcaacacgct
     3721 ctcggtgatc tccagttcca gccattgcgg gtcaagcgcg tgcatctgca gcacggccga
     3781 cagcacttgc tcgaagtccg gctgcgcgaa ctgcacggcc gagacgttca ccgccaccgg
     3841 cacgcagggc aggcccgcgc gccgccacgc ctgcagctgc cgggcggcct cgttcaacac
     3901 ccacttgccg atgttgacga tcagccccgt ctcttccgcc agcggaatga acttcgccgg
     3961 tgggacgagc cccagcgtcg gatgctgcca gcgcagcagc gcttcgaggc cggtgatctg
     4021 atcgtcggcg ttgaccttcg gctggtaatg caggcgaagc tcgccgttct cgaccgcccg
     4081 gcgcagcgcg ttctccaggt ccagccgctc gcgcgtgccg ttgttcatgt cggccgtgaa
     4141 acaccgcatg cgatcgcggc cattgttctt cgccgcatac aacgcggcgt cggcgttctt
     4201 cttcagcgtg atcgagtcgc gcccgtcctt gggatacatc gccagcccgc agctgatcgt
     4261 gacgaacagt tcctgcccca gaacgtccag cggcgccttg aacgcggcga tcaatcgctc
     4321 accaaatcgc tgggcgtcgc cggcgtcgct caggtccggc aggatcagcg cgaactcatc
     4381 tccgcccatt cgcgccatcg tgtcggtctt gcccagcgtt tccaacacgc gcgccaccag
     4441 ccggcagagc atcacgtcgc cggcgtgatg gcccagcgtg tcgttgatgt tcttgaaacg
     4501 atcaacgtcc aggaacgcca cgccgacggc ttttccggag atgtgcgcgc gaagcagcgc
     4561 ctgctgcagg cgatcttcga acaacacgcg attgggcagg ccggtcagcg catcgtgctg
     4621 cgcgcgatag gccagctgat cggtcagctg atgatgctcg accgcgatca tgatcagctt
     4681 ccccgccatc ttcaggaacg acgcgacgct gttatccaac tcgacgcgcg agcagtggta
     4741 caggcagaag acgccgagca cgtcgtcgtg gccggacttg atcagcgacg cccagcaggt
     4801 ctgcaggccg gtggccagcg caacgcggcg caccggttcc cagacgggat cgcgctccac
     4861 atcgctcgtg aagacgtcgc cgccgcgcat cgtggcatcc gtgcaaaagc gcgacgtgaa
     4921 ggaatagatg cgctcctgga gcgccgcctg caattccggc gggagcttgc cgctggcgtg
     4981 gtgcagctca ccatcaaaca gcaagatgca tcccgcggcg gcttcggggc atagccgctc
     5041 ggcggccttc acaagatgac tcatcacgtc gaccatcggc cggttctgca ccaccatctc
     5101 cagcacttcg gcccggtcct cttccatctg ctggatgcgc atgcgctggc tgatgtcgcg
     5161 cgccgatccg tggatctcgc gcaccccgcc gccctcaccg gccagcacgc ggaacgtaaa
     5221 ttcgagccag acgtaatgcc c
//
LOCUS       DATIMH010000497         3999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4159237, whole genome shotgun sequence.
ACCESSION   DATIMH010000497 DATIMH010000000
VERSION     DATIMH010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4159237"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VLI90_08675"
     CDS             <1..1022
                     /locus_tag="VLI90_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV14320.1"
                     /translation="VYTDGSDVTFNDTNNAHYLVTLNTTVSPHSTTVSNNATAYTISG
                     TGGIAGTGGLTKSGTGSLTISTVNTYSGNTNVTGGVMTIAAGGSIASNVVNVASGATL
                     NVNGSLSATAAVTANGPVNFGGTTSNTPLTRTIASLTVAPAVTVSITPSTNALTPVTL
                     QTGSLSITGTGKVDVGNNALIAPGSAASALTMIQGGQVVSSSIADSNHALGYITTGGT
                     NFEIRYTLKGDANLSGNVDVGDLGALATSYGITGGMSWANGDFNQDGTVNVADLGALA
                     TNYGTNLGTSPSLGGGGLSAAEPLAQAASSGGSAAVPEPTSLGLLSVAAVGLMGRRRR
                     RSASK"
     gene            1155..2999
                     /locus_tag="VLI90_08680"
     CDS             1155..2999
                     /locus_tag="VLI90_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HSV14321.1"
                     /translation="MVITPSVSALNDLPRGSSSPYLLSPEHAEKFQLEGAERHLGTLK
                     LVDVQQQPFAQALRISTRVGATQEWHVRVATDLPATVQAGSTLLARFWVRCVETTTGQ
                     GVVGFTCELNHGADEKLAEKRLSFDGEWIECFVPFKVRQRIAAGEGRICLNVGYDHQT
                     IELGGLSVMDCGTDVPVADLPRTQLSYPGRAATAEWREAAAERIERYRKGNLIVEVAD
                     DAGVPIAGAAVHATLRRHTFGFGTAVTARRLNDASPDGIQYRRIVESLFNQATFENDL
                     KWPAVYDGVPPETDQAAEWLRERQIQIRGHNLLWPSWRWLPQQLQKYRDDPIALRRRV
                     AEHVSELVAHFRGQLIHWDVVNEPFSERDLLELLGQDEMVAWFKLARQADPNAKLFLN
                     DYGIFGGGDGSNGHAPNEHADHFYDTLWFLRDKGAPLDGIGIQSHFTADLPSPQAMLA
                     TLERFGKLGLPIESTELTINLEDRQLQADYLRDYMTLMFSQPNVGGITLWGFWAPDHW
                     RPHSALFDEDWSIRPIGAAFEELVRHRWVTNTSLETDASGRASIRGFCGSYDVTVTTV
                     DGHTRKSTAVLDNDGGKLRVTIDRTPSHPRPPRRLVIGGGSEQGAANV"
     gene            2992..>3999
                     /locus_tag="VLI90_08685"
     CDS             2992..>3999
                     /locus_tag="VLI90_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSV14322.1"
                     /translation="MSDMHSSRFKALPPIDGWPEGVDTLATPLETRRRSGKSLRRRVT
                     IIDVARQCGVTPATVSRVLNNKKKFSTTEVVRTKILETARKMGYVPDLSARNLNRGTT
                     NIVGLFASPLTHVAEGINEPLLEGMADVLHNGGFEVFFEFGAITRAQASLPFWRFDGA
                     IVMQAPKPGTIRELDHRQVPYVCVNEQVGQPAASVLADDPMGMRRAVDHLAMLGHRRL
                     AYANARAEYFAHYSVTERYETLLQQAKERSLQLASGHDTPFASGVDFLRGAVQSAGAT
                     AIITYDHRIAVALMGAAHELRLRIPDDFSLICFNDVFPVATLGPPLTAVAVSGREMGR
                     VG"
BASE COUNT          732 a         1277 c         1315 g          675 t
ORIGIN      
        1 cggtttacac cgatggctct gatgtcacct tcaatgacac caacaatgcc cattacctgg
       61 tgacgcttaa tacgaccgtc tcaccgcact cgacgacggt ttccaacaat gccaccgcgt
      121 acacgatctc cggcacaggt gggatcgccg gcacgggtgg gctgaccaag agcggcacgg
      181 gatcgctcac catttccacc gtcaacacct atagcggcaa cacgaacgtg accgggggcg
      241 tgatgacgat cgccgccggg ggttcgatcg ccagcaacgt ggtgaacgtc gccagcggcg
      301 cgacgttgaa cgtcaacggt tcgctgtcgg cgacggcggc ggtcaccgcg aatggcccgg
      361 tgaatttcgg tggcaccacc agcaacacgc cgctcacgcg gacgatcgct tcgctgacgg
      421 tggcgccggc ggtgacggtg tcgatcacac cgtcaaccaa tgcgttgacg ccggtgacgc
      481 ttcagacggg aagtctgtcg atcaccggca cgggcaaagt cgatgtcggt aacaacgcgc
      541 tgatcgcgcc gggcagcgcg gcgtcggcat tgacgatgat ccaaggtggt caggtggtca
      601 gctcgtcgat cgccgattcg aatcacgcgc tgggctacat cacgaccggc ggcacgaatt
      661 tcgagatccg ctatacgctt aagggcgatg cgaatctcag cggtaacgtc gatgtcggcg
      721 atctgggcgc gctggcgacg agctacggca tcaccggcgg gatgagctgg gcgaacggcg
      781 atttcaacca ggacggcacc gtcaacgtcg ccgacctggg cgcgctggcg acgaactacg
      841 gtacgaacct cggcaccagc ccgtcgctcg gcggtggtgg cttgtccgct gccgagccat
      901 tggcgcaggc ggcgagcagc ggtggttcgg cggccgtgcc tgaaccgacc tcgcttggac
      961 tgctgtcggt tgcggcggtg ggcctgatgg gccggcgccg acgccggagc gcttcgaagt
     1021 aatcggcgcg catcggcgtt gtaacgcgaa ctggacctcc gctgcggcgg cccgtacctg
     1081 accggcgggc cgccgccctc tggaggttcg cgcaacagtg atgacagcac cggcagaata
     1141 cgtgaagtaa caacatggtc attacacctt ctgtatccgc gctgaacgac ctgccgcggg
     1201 gatcgtcgtc cccgtacctg ctctcgccgg agcacgctga aaagtttcag ctcgaagggg
     1261 ccgagcggca cctgggaacg ctgaagctgg tcgacgtgca gcagcagccg ttcgctcagg
     1321 cgcttcgcat ttcgacacgg gtgggggcga cacaggaatg gcacgttcgc gtggcgacgg
     1381 atctgccggc gacggttcaa gcgggcagca cgctgctggc gcgcttctgg gtgcgctgcg
     1441 tcgagacgac caccgggcag ggcgtggtcg ggttcacctg cgaattgaac cacggcgccg
     1501 atgaaaagct ggccgagaag cggctgagtt tcgatggcga atggatcgag tgcttcgtgc
     1561 cgttcaaggt ccgccagcgg atcgccgcgg gggaaggccg catctgcctg aacgtcggct
     1621 acgaccatca gacgatcgag ctgggcggcc tctccgtcat ggactgcggg acggatgtgc
     1681 cggttgccga tctgccgcgg acgcagctga gttatcccgg tcgcgccgcg acggccgagt
     1741 ggcgcgaggc ggcggcggag cggatcgagc gctatcgcaa agggaacctg attgttgaag
     1801 tcgcggacga cgctggagtg ccgatcgctg gcgcggctgt acacgccacg ctgcgccgcc
     1861 acacgttcgg cttcggtacc gcagtcaccg caaggcgcct caacgacgct tcgcccgatg
     1921 gcattcaata tcgccgcatc gtcgaatcgc tcttcaatca ggcgacgttc gagaacgacc
     1981 tgaagtggcc ggcggtatac gacggcgtgc cgccggaaac agaccaggcc gcggaatggc
     2041 tgcgcgagcg gcagatccag atccgcggac ataatctgct ctggcccagt tggcgttggc
     2101 tgccgcagca gctgcagaag taccgggacg atccgatcgc gctgcggcgt cgcgtcgccg
     2161 agcacgtcag cgaactcgtc gcgcacttcc gtggccagtt gatccactgg gatgtggtga
     2221 acgagccgtt ctccgagcgc gacctgctcg agctgctcgg gcaggacgag atggtcgcgt
     2281 ggttcaagct ggcgcggcaa gcggacccca acgcaaaatt gttcctcaac gactacggca
     2341 tcttcggcgg cggtgatggc agtaacggac acgcgcccaa cgagcacgcc gaccattttt
     2401 acgacacgct ctggttcctg cgggacaagg gggccccgct ggacggcatc ggcatccagt
     2461 cgcacttcac cgcggatcta ccgtcgccgc aggcgatgct cgccacgctc gagcgcttcg
     2521 gcaagctcgg cctgccgatc gaatcgacgg agctgacgat caacctcgaa gaccgccagc
     2581 tgcaggcgga ttacctgcgc gattacatga cgctgatgtt ctcgcagccc aatgtcggcg
     2641 ggatcacgct gtggggattc tgggcgccgg atcactggcg tccgcactcg gcgctgttcg
     2701 acgaggactg gtcgatccgc ccgatcggcg ccgcgtttga agaactcgtc cgccatcgtt
     2761 gggtgaccaa tacctcgctt gaaaccgacg ccagcggccg cgcgtccatc cgcggattct
     2821 gcggcagcta tgacgtcaca gtgacgaccg ttgacggtca cacccgcaag tcaaccgccg
     2881 tgctggataa cgacggcggg aagctgcgcg tgacgatcga tcgaacgccg agccaccccc
     2941 ggccgccgcg gcggctggtg atcgggggcg gcagcgagca gggagccgcc aatgtctgac
     3001 atgcattcat ctcgattcaa ggcgctgccg ccaattgacg gttggcccga gggggtcgat
     3061 actctggcaa cgccgctgga aacccggcgc cgctcaggga aatcattgcg acgccgcgtc
     3121 acgattattg atgttgcccg ccagtgcggc gtcactcccg caaccgtcag ccgagtcctc
     3181 aacaacaaaa aaaagttctc caccacggaa gtggttcgaa ccaagattct ggagacggcc
     3241 cggaagatgg gctatgttcc tgatttgtcc gcccgcaatc tcaatcgcgg cacaaccaat
     3301 attgtcggtc tgttcgcctc gccgctgacg cacgtggccg aaggcatcaa cgaaccgctg
     3361 ctcgaaggca tggccgacgt cctgcacaac gggggattcg aggtcttctt cgagttcggc
     3421 gcgatcacgc gcgcacaagc gtcgctgccg ttctggcgat tcgacggcgc gatcgtcatg
     3481 caggccccca agcccgggac gatccgcgag ttggaccatc gccaggttcc atacgtctgc
     3541 gtgaacgagc aggtgggcca acccgcggcg tcggtgctgg cggacgaccc gatgggcatg
     3601 cgacgggcgg tcgatcacct ggcgatgctg ggtcatcgtc gcctcgccta tgcgaatgcc
     3661 cgcgcggaat acttcgcgca ctacagcgtg accgagcgct acgagacgct gctgcagcag
     3721 gcgaaggagc gatcgcttca actggccagc ggtcacgata cgccgttcgc ctccggcgtt
     3781 gatttcctgc gcggcgcggt gcaaagcgcc ggcgcaacag ccatcatcac atacgaccat
     3841 cgcatcgcgg tggcgctaat gggggcagcc catgaactgc ggctgcgcat ccccgacgac
     3901 ttcagcctga tctgcttcaa cgatgtgttc cccgtcgcca cgctcggccc gccgctgacc
     3961 gcggtggccg tatcgggccg cgagatgggt cgtgtcggc
//
LOCUS       DATIMH010000498        14263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_984748, whole genome shotgun sequence.
ACCESSION   DATIMH010000498 DATIMH010000000
VERSION     DATIMH010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 14263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14263
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984748"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VLI90_08690"
     CDS             complement(<1..233)
                     /locus_tag="VLI90_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14323.1"
                     /translation="MKRLSIVLLLLMTFGCAAPRTESAPVTSTQPTTAPSTQPTAVTV
                     SDDGETYTLSNGIVTARILKHSGAMASLVYQNI"
     gene            complement(317..2686)
                     /locus_tag="VLI90_08695"
     CDS             complement(317..2686)
                     /locus_tag="VLI90_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSV14324.1"
                     /translation="MRLALVLVILLGAVAGPALAASVALADNGRTTQSFDADWRFFKG
                     DASGADQPAFDDSTWRSLSVPHDWSIEGPFDQKNPTGPAGACLPAGIGWYRKHFILPA
                     NGAGRRVFINFDGVMANSDVWINGILLGHRPYGYVSFQYELTDHLNTGDGASNVIAVR
                     ADNAQQPASRWYAGAGIYRHVHLVVTDAVHIDHWGTTVTTPRVGKIDADVHVRSHVVN
                     QSSAARGVSVQVNLVDADGKTVATVESSPQMIDAGKAVDVEQDLNRLKLRLWDLDEPY
                     LYRAMASVRDGGATIDEDAAAFGVRSAEFNADTGFWLNGKNIKLKGVCLHGDAGGLGV
                     AVPISAWRRRLQSLKDVGCNAIRTAHNPPAPEFLDLCDQMGFLVMDEMFDCWTVPKNP
                     YDYHLYFKDWSLIDTRDTVRRDRNHPCIVLYSAGNEIHDTPHAEIAKPILASLIDVFH
                     KEDPTRPVTQALFRPNVSHDYDNGLADMLDVVGQNYRENEIVAAHEQKPTRKIVGTEN
                     AKDLKAWAAVRDHAFHSGQFIWTGIDYLGEGRRWPRIGNPSGLFDTTGNAYSIAFERR
                     SWWTDKPTVLIARDEGNVPTGAMAGEPQTRRVERLDWTPKNTEPHVERVVVYSNGDSV
                     ELSLNGKSLGAQDRPRDESPRAWQVNYEPGTIKAVATMRGKWEATQEWRTAGKPARIA
                     LKAEIEKFTPSWDDVSYVIANVVDDQGTIVPSADDRIAFKIDGPGAIAAVDNGDLTSH
                     EPFQASERHAYHGRCVAVVRATAATGQITLTASAAGLQPSSCTLNVAPE"
     gene            2922..4229
                     /locus_tag="VLI90_08700"
     CDS             2922..4229
                     /locus_tag="VLI90_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14325.1"
                     /translation="MAESSDRFSCASCQRSYIWKPALAGKRVKCKCGAMMDVPSGVMP
                     QAAPQLKPQVIHRPRPAPVAAASQADDDALYAMADMEAKAAANLPPMVMAAAPVAAVA
                     MAVPGRATTVSGVPLAYRRGPSAKERERAGTNVLIDMNRDVYVPTAMLVGGFLIYAGY
                     YAITYHLGGVAIVITMFGLSLLTAFKAAILVGFALVAATPLGVSFGGVFTAILKLAAI
                     AVFCDGVTTWIDAGVAHISGGAGGGGFFGMGIISWPIALGMYWGFFIYLFSMDPGDSW
                     MVVMILSIIDYLARWVLIALLLSAVLNWGGVASGPIASIGGGGSSKSQASATEEHVTE
                     LKESGGLRDARQYITDGHQAGLGPFVESCFAAGAKSVSFEVSRDINGKTDPYALAIEL
                     PSEKSKRAAVYAAIKRHYDEAKVEYDPEDLKDDGERYVEVELK"
     gene            4619..5185
                     /gene="ilvN"
                     /locus_tag="VLI90_08705"
     CDS             4619..5185
                     /gene="ilvN"
                     /locus_tag="VLI90_08705"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HSV14326.1"
                     /translation="MRHVISALVMNEPGVLANVAGMFAARGFNIDSLVVGRTENPDLS
                     RMTIVVTADENTLEQVRKQLAKLVPIVKVRDFKDTSYIERDLALITVGVSAEHRGEVI
                     EMANLFRAKIVDVSKTSVIIEMSGTEEKIEAFIELMKPYGITELARTGVIAMARGMQI
                     GKDTGPAAGTKQRKRSMDAPAAAALPPS"
     gene            complement(5262..6347)
                     /locus_tag="VLI90_08710"
     CDS             complement(5262..6347)
                     /locus_tag="VLI90_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HSV14327.1"
                     /translation="MTELASPQTLSSMNGKPLLQVRDLRTHFPIRRGVFSRTVGFVKA
                     VDGVSFDVHAGKTLGLVGESGCGKTTVGRTILRLIPATSGSVLYNGEDFFQPHGEQLR
                     QLRRKMQIVFQDPVSSLNPRMRIGNIIGEPIEVHHIARGRERDELVASLLQRVGLDPS
                     YAARYPHEFSGGQRQRIGIARALALSPDFIVCDEPVSALDVSIQSQILNLLDDLQKER
                     KIAYLFIAHNLAVVKHFSDEVAVMYLGRIVEKASAKELYANPKHPYTKSLLSAVPEPN
                     PRPTKRRIVLSGEVPSPANPPSGCPFHPRCPLTRMMAKESSDAETVQIKSAGQSVRVM
                     RRCVAEPQVLEQKEGEPGHVAACWVEK"
     gene            complement(6584..7048)
                     /gene="rpiB"
                     /locus_tag="VLI90_08715"
     CDS             complement(6584..7048)
                     /gene="rpiB"
                     /locus_tag="VLI90_08715"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HSV14328.1"
                     /translation="MKLAVACDHRGYEAKRRLLPLLKKLGHEIQDFGCDSTTAVDYPD
                     LAAPAAEAVASGACDIGVLLDGSGIGMSIAANKIDGVRAALVHDEVTARRAREHNHCN
                     VLCLGTDLLSEEHIRNIVEIFLATEYGNGRHTRRVEKLKGLEQRNRAHMHQS"
     gene            complement(7088..8215)
                     /locus_tag="VLI90_08720"
     CDS             complement(7088..8215)
                     /locus_tag="VLI90_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HSV14329.1"
                     /translation="MPTPIVHIFDTPDYDADIRRAVSVVGGGGLVVLPTETVYGAAGL
                     LNHPQGKAKLQALRDGGAPAKPFTIHLAHRDAAAQYIAPANDFAKRLMRKLWPGPVGL
                     MFDVPAERRAQVAAALNVPESDLYSDGRITLRCPDHMVTTDILAEVNGPVALTMAGAA
                     AGGSNIRFDAMAQELDGKVDLIFDAGPTAYAKPSTLLKVNEKNYEIVRAGVYDERIIE
                     RLLKTTILFICSGNTCRSPMSEAIARNVIAKKLNVPEADLERKGINVISAGSFAMPGA
                     RATQQGVEALREMGIDLTRHRSRPLSVELIHQADWIYGMGRSHVQAVRSLVPSAADKA
                     QTLNPDGDIEDPIGSDVAVYRDLAGQLRSLIEKRLDGITLA"
     gene            complement(8475..10424)
                     /locus_tag="VLI90_08725"
     CDS             complement(8475..10424)
                     /locus_tag="VLI90_08725"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSV14330.1"
                     /translation="MHLWFLLPFPNPANAWFDAAWPYRRTIDVTWDAEHGSGDQLAAA
                     EFYSDGHTLPNADDVRVASEDGKLVPTHILQDGPGDRIRLAFTLKKGVKKYAVYFGNP
                     KPEASKETEDVKWTAGLMLETRAWSGGGAGSAADLERSWNRNTRTLGQAVIPTPFIGY
                     NYFGPQEQWISKFIGSLFAPIDGQYAFAISVDDSAALFIDGKPLLFAPIGPPDVRYNA
                     AVHLSRGRHELLLYHLNTGGDGRVAVAWQRPDNGKFELIPREAFGICYGCQVGQLEQQ
                     GRSLLADFAAEQIGECFVADHYSFRYHFKAHSRAASPLKYEWDFGDGQTATQPDVDHV
                     FLDADVLPVRLTVRVGPNSDTQTIRLPISRLWDRITTPPSEDPPAQSKVIADYDLTKM
                     PPNDLMRATLLHLRAQKLDAALSTGDELAKSQTVPQPAAAYDALLELDRALLQAGQPN
                     EVAKLWDRVPVDSQLQPRAARRAAQIAMWWAGDLEKAVKLLEPLTRRHDHATERLYGE
                     ALILAGNGERGRQVLLDLPEQGQTDRAAALSGASARSVEYFITEGDAEAGEDAWERWQ
                     TKFPADFVEGYSLLLRVKLIELRKQPTTAARVAEAFATAMPKSSYAPQLLDRASKLLA
                     KTDSKKSAALRQLLKQKYPEDPLSQ"
     gene            10619..11203
                     /locus_tag="VLI90_08730"
     CDS             10619..11203
                     /locus_tag="VLI90_08730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSV14331.1"
                     /translation="MMLPKAILFDMDGTLTQPYMDYDRIKRDMGVGPGPILESLAAME
                     PQQRHAAEAILHQHEDAAASASTLNPGCVELLTWLREIGLETALVTRNSRRSVRTVFD
                     LHGLHFDVCVTREDGKFKPDPAPLLLACQRLGVEPDKTWMVGDSYHDIDAGIAAKIRT
                     VWVSHGKAKAFASEPWRTVRDLLELTQVLKACLK"
     gene            11301..12107
                     /locus_tag="VLI90_08735"
     CDS             11301..12107
                     /locus_tag="VLI90_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14332.1"
                     /translation="MRVILICVLLVMSTMALAQPAPPLINPVGAGADSATLRPDAGMD
                     EVLEALHERGQNLKEFVADVTLIDNDLIMSTQTTRTGKVWAQFKPGGDARLHARFDKR
                     IEEGKPPQNDLKEYSLDDGWLVDRDYRAKNEVRRQVAAPGEKVNLFQLGKGPFPLPIG
                     QEVKSVHEQFDVTKVEPAKDDPPGAIHLQLTPKEKTDLARKFQVIDVWVDPATRMPVR
                     IKTIDHNQTTERQTDFTNVKVNPTPGLTDADFKLPPIDQSGWQRHEEALH"
     gene            complement(12287..12904)
                     /gene="hisH"
                     /locus_tag="VLI90_08740"
     CDS             complement(12287..12904)
                     /gene="hisH"
                     /locus_tag="VLI90_08740"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HSV14333.1"
                     /translation="MSIAIIDYGMANLRSVQKALAEVGHAADIISTPEQIERADKIVL
                     PGVGAFQDAVATLRDKRLADPIVHHIEKGKPFLGICLGLQMLFDVGYEDGEHRGLGVI
                     RGKCVRFDVDSTLGLKVPHMGWNQLNRRSNTPLLRDLKEGCSVYFVHGYHVVPEDESI
                     IATTTDYGRPFVSSIWRDNVMATQFHPEKSQKVGLQVLANFAGLS"
     gene            complement(12939..13496)
                     /locus_tag="VLI90_08745"
     CDS             complement(12939..13496)
                     /locus_tag="VLI90_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14334.1"
                     /translation="MSKLVSRILLAIFMLPLATVVYMLAAIAIEESMGYPHENGMFIG
                     AGIVTWGFVAGYWLLLWGKSIAWTPRRIWLTVAAAGALLLLALGATTLAYGTSRTEGE
                     ITVCFSSILAPLWWLIFTVFIWRESAAERTMRIKRAGGSAIACPNCGYNLTGLAESRC
                     PECGSRFTLDELLAAQPAAMTAEID"
     gene            13872..>14263
                     /locus_tag="VLI90_08750"
     CDS             13872..>14263
                     /locus_tag="VLI90_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEPR-XLL domain-containing protein"
                     /protein_id="HSV14335.1"
                     /translation="MRNEHQASSSSSPRRSKSKTRRPGRSTPRRDRSNLFEIFPLEKR
                     VLLSGTPTTFDHTFRYLVAPALTPAPSPHVTGSATPVGLNAAQIRGAYGVDNISFSNG
                     ALGTGAGITIAIIDAYDTPSIIGDLHTF"
BASE COUNT         2556 a         4621 c         4458 g         2628 t
ORIGIN      
        1 tcgatgttct ggtaaacgag cgacgccatc gcgccgctgt gcttgaggat ccgcgcggtg
       61 acgatgccgt tggagagcgt gtaggtctcg ccgtcgtcgg agacggtgac ggcggtaggt
      121 tgagtggacg gggcggtggt aggctgcgtg ctggtgaccg gcgcagattc cgtgcgaggc
      181 gcggcacaac cgaacgtcat aagcagcagc aggacaatcg acaatcgctt catcgcgcga
      241 tccttgatag ccgggggcgg aagccccggt cttttaggtc ttgtgttcat gaacagaccg
      301 gggcttccgc ccccggctat tcaggagcaa cgttcagcgt gcacgaactc ggctgcaatc
      361 ctgctgccga cgccgtcaac gtaatctgcc ccgtcgccgc agtcgcccgc accaccgcaa
      421 cgcaccgccc gtgatacgcg tgccgctcgc tcgcctggaa cggctcgtgg ctcgtcaggt
      481 cgccattgtc caccgcggcg atcgcgccgg ggccgtcgat cttgaacgcg atgcgatcat
      541 ccgccgacgg aacgatcgtc ccttggtcgt ccacgacgtt ggcgatcacg tagctgacgt
      601 cgtcccagct cggcgtgaat ttctcgatct ccgccttcag cgcgatccgc gccggcttgc
      661 ccgccgtccg ccattcctgc gttgcctccc acttcccgcg catcgtcgcc acggccttga
      721 tcgtgccggg ctcgtaattc acctgccaag cgcgcggcga ttcatcccgc ggccgatcct
      781 gcgcgcccag cgacttgccg ttcagggaca gctccaccga atcgccgttg ctgtaaacga
      841 caacgcgttc gacatgcggc tcggtattct tcggcgtcca atccaaccgc tccacccgcc
      901 gcgtctgcgg ttcacccgcc atggcgccgg tcggcacgtt cccttcatcg cgcgcgatga
      961 gaacggtcgg cttgtcggtc caccagcttc gccgttcgaa cgcgatggaa taggcgttgc
     1021 ccgtcgtgtc gaacaagccc gaaggattgc cgatgcgcgg ccagcgccgg ccttcgccca
     1081 ggtaatcgat gccggtccag atgaactgcc cgctgtgaaa cgcgtgatcg cgcacggccg
     1141 cccatgcctt caggtccttg gcgttttccg tgccgacgat cttccgcgtc ggcttctgct
     1201 cgtgcgcggc gacgatttcg ttctcgcggt aattctgccc gacgacgtcg agcatgtcgg
     1261 ccagcccgtt gtcgtaatcg tggctgacgt tcggccgaaa cagcgcctgc gtcaccggcc
     1321 gcgtgggatc ttccttgtga aatacatcga tcagcgacgc caggatcggc ttggcgatct
     1381 ccgcgtgcgg cgtgtcgtgg atctcgttgc ccgcgctgta cagcacaatg cacggatgat
     1441 tgcggtcccg ccgaaccgtg tcgcgcgtat cgatcagcga ccagtccttg aagtacaggt
     1501 gatagtcgta cggattcttc ggcaccgtcc agcagtcgaa catctcgtcc atgacgagga
     1561 agcccatctg atcgcacagg tccagaaact ccggcgccgg cggattgtgc gctgtgcgaa
     1621 tcgcgttgca gccgacgtcc ttcagcgact gcaaccgccg ccgccaggcg ctgatcggga
     1681 ccgccacgcc aagcccaccg gcgtcgccgt gcaggcagac gcctttgagc ttgatgttct
     1741 taccgttcaa ccagaaaccg gtgtcggcgt tgaactccgc cgaccgcacg ccgaacgccg
     1801 cggcgtcttc gtcgatcgtc gcgccgccgt cgcgaacgct cgccatcgcg cgatacagat
     1861 acggctcgtc caaatcccaa agccgcagct tcaaccgatt tagatcctgc tcgacatcga
     1921 ccgctttccc cgcatcaatc atctgcggcg acgattcgac cgtcgcgacc gtcttcccgt
     1981 ccgcatcaac aagattcacc tgcacgctca ccccccgcgc ggcgctcgat tggttgacga
     2041 cgtgactgcg cacgtgcaca tccgcatcga tcttcccaac gcgcggcgtc gtaacggtgg
     2101 tgccccagtg atcgatgtgc accgcatcgg tcaccacgag atgcacgtgc cgataaattc
     2161 cggcaccggc gtaccagcgc gacgcgggtt gctgcgcgtt gtccgcgcga acggcaatca
     2221 cgttgctcgc gccgtcgccg gtgttcaggt ggtccgtcag ctcatattga aagctgacgt
     2281 acccatacgg ccgatggccg agcaggatgc cgttgatcca cacgtcgctg ttggccatca
     2341 cgccgtcgaa gttgatgaag acgcgccggc cggcgccgtt cgccggcaga atgaaatgct
     2401 tgcgatacca cccgatgccc gcgggcaggc acgcccccgc ggggccggta gggttcttct
     2461 gatcgaaggg gccttcgatg ctccagtcgt gcgggacgct gagcgatcgc caagtggaat
     2521 cgtcgaacgc gggctgatcc gcgccgctgg cgtcgccttt gaagaaccgc caatccgcat
     2581 cgaacgactg cgtggttcga ccattgtcgg ccagcgcgac cgacgcggcc aacgccgggc
     2641 cggcaacggc gccgagcagg atgacgagca ccaacgcaag ccgcatcgcg gttcctccag
     2701 agggcggaaa tttgtacctc tagtacccca tccgcgtgcg gatgtttgaa tcgggaaacg
     2761 gaattcatcg cgcgacaaaa gtccatctcg tcgcgacttg cgcggcgcgg actgacgcgt
     2821 catccatcgc agccgcctgc tccaaaaaaa ccgcttgcca aactttcgac acttggcatg
     2881 atttcgcagg attcgatccg ttcttccaat cggggtgcaa catggctgaa tcatccgacc
     2941 gattttcctg cgccagctgc cagcgctcgt acatctggaa acccgcactc gccggcaagc
     3001 gcgtgaagtg caagtgcggc gcgatgatgg acgtgcccag cggtgtgatg ccgcaggctg
     3061 cgccgcaact gaagccgcag gtcattcatc gacccaggcc cgcgccggtt gcggccgcgt
     3121 cgcaagcgga cgacgatgcg ctctacgcga tggctgacat ggaagccaag gccgcggcta
     3181 acctgccgcc gatggtgatg gccgccgccc cggtggccgc ggtggcgatg gcggtgccgg
     3241 ggcgcgcgac gacggtcagc ggggtgcctc tggcgtatcg gcgcgggccc agcgccaaag
     3301 agcgcgagcg cgccggcacc aacgtgctga tcgacatgaa ccgcgatgtg tacgtgccga
     3361 cggccatgct cgtcggcggg tttctcatct acgcggggta ctacgcgatc acctatcacc
     3421 tcggcggcgt tgcgatcgtc attacgatgt tcggcctgtc gctgctgacg gcattcaagg
     3481 cagccatcct ggtcggcttc gcgctggtcg cggcaacgcc gctcggcgtc agttttggtg
     3541 gggtcttcac cgccatcctg aagctcgccg ccatcgcggt cttctgcgac ggcgtcacca
     3601 cctggatcga cgcgggcgtc gcgcatatct ccggtggcgc cggcgggggc gggttcttcg
     3661 gcatgggcat catcagctgg cccatcgcgc tgggcatgta ctgggggttc ttcatctacc
     3721 tcttcagcat ggaccccggc gactcatgga tggtcgtcat gatcctcagc atcatcgact
     3781 acctcgcccg atgggtgctc atcgcgctgc tgctcagcgc ggtcctgaac tggggtggcg
     3841 tcgcctcggg tcccattgcc agcatcggtg gcggtggcag cagcaaatct caggcaagcg
     3901 ccaccgaaga acacgtcacc gagctgaaag aaagcggcgg cctgcgcgac gcacggcaat
     3961 acatcaccga cggccaccag gcgggcctcg gcccgttcgt cgaatcctgc ttcgccgccg
     4021 gcgccaagag cgtctcgttc gaagtctcac gcgacatcaa tggcaagacg gatccgtatg
     4081 cgctggcgat cgagctgccg agcgagaagt ccaagcgagc cgcggtgtac gcagccatca
     4141 agcgacatta cgacgaggcg aaggtcgagt atgacccgga ggacctgaaa gacgatggcg
     4201 agcggtatgt tgaagtggaa ttgaagtgat ggatcaatgt ggcacgggct accagcagct
     4261 accagcccct gttgcgtggt accccacgaa tgtcggcgat actccaccga cgaagcattc
     4321 gcgccagcga agagacttcg gaatccgtcg ctgacgcgat tgccgtgtcg atggcgcacc
     4381 atcgccatgc gtgaggacac gcatcatggg caagatgcgc gcgtaactga tgttgtaggc
     4441 actcacgcag ctcttcccgc ccagaggtcg gaccacagga tctgttttat caaaagtgcc
     4501 tatttcgcgc gatagacccc ccggtgtctg gcctcccatc cccacccatc cccaccttgc
     4561 ccccacgcgt gaatcgctta gactttgtcc cgtctatctc ttttcggagt cttgtaacat
     4621 gcgtcacgtg atctcggcgt tggtgatgaa cgaaccgggc gtgctggcca acgtggccgg
     4681 catgttcgcc gcccgcgggt ttaatatcga ttcgctcgtc gtcgggcgga ccgaaaaccc
     4741 cgacctgtcg cgcatgacca tcgtcgtcac cgccgacgaa aacacgctgg agcaggtgcg
     4801 caagcagctg gccaagctgg tgccgatcgt gaaggtgcgc gacttcaagg acacgtccta
     4861 catcgagcgc gacctggcgc tgatcacggt cggcgtgtca gccgagcacc gcggggaggt
     4921 cattgagatg gccaatttgt tccgcgccaa aatcgtggac gtatccaaga ccagtgtgat
     4981 catcgagatg agcggcacgg aagaaaagat cgaagcgttc atcgagctga tgaagccgta
     5041 cggcatcacc gagctcgccc gcaccggcgt gatcgcgatg gcccgcggga tgcagatcgg
     5101 caaagacacc ggccccgccg cgggcaccaa gcagcgcaag cgcagcatgg acgctcccgc
     5161 ggccgcggcg ctgccgccga gttgatatcg ctacccgctg ctcgactcgg ccaactctgg
     5221 cgcgcgtcga tgcgcgctgg tgctctggca agacctcgcc gtcacttctc cacccaacac
     5281 gccgcgacgt ggccgggctc gccttccttt tgctccagca cctgcggctc cgccacgcac
     5341 cgtcgcatca cgcggacgct ttggccggcg gatttgatct gcaccgtctc cgcgtcgctc
     5401 gattccttcg ccatcatccg cgtcagcggg cagcgcgggt gaaatgggca gccgctcggc
     5461 gggttcgccg gcgacgggac ttcgccgctc aacacgattc gccgcttggt cggccgcggg
     5521 ttgggttcgg ggaccgcgga taaaaggctc ttcgtgtatg ggtgcttggg gttggcgtac
     5581 agctctttgg cgctggcctt ctcgacgatt cttcccaaat acatcaccgc gacttcgtcg
     5641 gaaaaatgtt tgaccaccgc caggttgtgc gcgatgaaca ggtacgcgat ctttcgctct
     5701 ttctgaagat cgtcgagcaa attcaaaatc tgcgattgga tcgacacgtc cagcgccgac
     5761 accggttcgt cgcagacgat gaagtccggc gacagcgcca gcgcccgcgc gatgccgatg
     5821 cgttgccgct gcccgccgct gaattcgtgc gggtatctgg ccgcgtagga tggatcaagt
     5881 ccgacgcgct gcagcaggct ggcgaccagt tcatcgcgct cgcggccgcg cgcgatgtga
     5941 tggacttcga tcggctcgcc gatgatgttg ccgatccgca tgcgcggatt cagcgagctg
     6001 accgggtcct gaaaaacgat ctgcattttt cgccgcagct ggcgaagctg ctcgccgtgc
     6061 ggctggaaaa aatcctcgcc gttgtacaac accgacccgc tggtcgcggg gatgaggcga
     6121 aggatggtgc gacccacggt cgtcttcccg cagccggatt caccgaccag accgagcgtc
     6181 ttccccgcgt gcacatcgaa gctgacaccg tcaacggctt tcacaaaccc gaccgtgcgc
     6241 gagaacaccc cgcggcggat ggggaagtgc gtgcgcagat cccgcacctg cagcagcggc
     6301 ttgccgttca tcgaggaaag agtttgcggt gaggcgagtt ccgtcattac agaatttcca
     6361 cacgaacaga tccgactgaa tcccactctt gtcccctctc ccggtactcc gggagagggt
     6421 tagggtgagg gtgatttcga gtgccagcgg ctatcgaaat tcaaaatcac cctcacccct
     6481 gccctctccc gcagacctgc ggtcagcagt accggaagag ggggaaggag ctatacaaag
     6541 aaaaagccca cggctatgcc gtgggctttg tggggtttat ttttcagctc tgatgcatgt
     6601 gcgcgcgatt gcgctgctcc aaccccttca acttctccac gcgccgcgtg tgtcggccat
     6661 tgccatactc ggtcgccagg aaaatctcga cgatgttgcg gatgtgctct tcactcagca
     6721 agtcagtgcc caggcacagc acgttgcaat gattgtgttc gcgcgcccgg cgcgcggtga
     6781 cttcatcgtg caccagcgcc gctcgcacgc cgtcgatttt gttcgccgcg atcgacatgc
     6841 cgatgccgct gccgtcgagg agcacgccga tgtcgcacgc gccgctggcc accgcctcgg
     6901 ccgcgggggc ggccagatcc gggtagtcca ccgccgtcgt gctgtcgcaa ccaaaatcct
     6961 ggatctcgtg gccgagcttc ttcagcagcg gcaacagccg gcgcttggcc tcgtacccac
     7021 gatgatcaca ggcaaccgca agcttcatcg actcctccgc acaacccgcg atactccaac
     7081 gcgcgcctca cgccagcgtt ataccatcta atcgcttttc aatcaggctt cgcagctgtc
     7141 cggccaggtc tctgtacacg gcgacgtcgc tgccgatcgg atcctcgatg tcgccgtcgg
     7201 ggttcagcgt ctgcgccttg tcggccgccg acggcacgag tgacctgacc gcctgcacat
     7261 ggctgcggcc catgccgtag atccagtccg cctgatggat caattctacc gataacggcc
     7321 gcgagcggtg gcgcgtcagg tcgattccca tttcccttag ggcttccaca ccctgctgcg
     7381 tcgcccgcgc gccgggcatc gcaaaactgc cggcggaaat cacgttgatg cccttgcgct
     7441 ccagatccgc ttccggcacg tttaactttt ttgcgatcac gtttcgcgcg atggcttcgg
     7501 acatcggcga gcggcacgta ttcccactgc agatgaacag gatcgtcgtc ttcagcagcc
     7561 gctcgatgat ccgctcatcg taaacgccgg cgcggacgat ttcataattc ttctcgttca
     7621 ccttcagcaa ggtggacggc ttggcgtaag cggtaggccc ggcatcgaag atcaggtcga
     7681 ctttgccgtc cagttcctgc gccatcgcat caaagcgaat gttcgatcca cccgcggcgg
     7741 cgccggccat cgtcagcgca acggggccgt tcacctcggc caggatgtcc gtcgtcacca
     7801 tgtgatccgg gcaacgcagc gtgatcctgc cgtcgctgta caggtccgac tccggcacgt
     7861 tcaacgccgc cgccacctgc gcccggcgct cggccgggac gtcgaacatc agcccgactg
     7921 gaccgggcca gagcttgcgc atcaaccgct tggcaaagtc gttggccggc gcgatgtact
     7981 gcgcggccgc atcgcgatgg gcgaggtgaa tcgtaaacgg cttggccggc gcgccgccgt
     8041 cgcgcagcgc ctgcagtttc gccttcccct gcgggtgatt cagcagcccc gcagcgccgt
     8101 aaacggtttc cgtcggcagc acgaccagcc caccgccgcc gacaacggag accgcccgcc
     8161 ggatgtcggc gtcgtagtcg ggcgtgtcga agatatggac gatcggcgtc ggcatggaaa
     8221 cgcaaccgat atatgaggcg agctaagctg gggtgtcaag gaagaggaat ctgcccgtag
     8281 cacgggcgtc tcgcccgtgg tgcgtgtact cacgcacggc gatggtacgc catcgacaca
     8341 gtaatcgcgt tagccagggc tttcgagtgc cctcgcttgc gcgattgctt catcgacggc
     8401 gtaccgtcga tattcgtgga gtaccacgaa acacgggcga ggcgcccgtg ctacggtacc
     8461 gctacgcggg gtgatcactg gctcagcgga tcctcgggat acttctgttt cagtaattgg
     8521 cgaagcgccg cgctcttttt tgaatcagtc tttgccagca gcttgctcgc gcgatcgagc
     8581 agctgcgggg cgtacgacga cttgggcatc gccgtcgcga acgcctcggc cacgcgggcg
     8641 gccgttgtcg gctgcttgcg cagctcgatc aacttgacgc gcagcaatag gctgtagcct
     8701 tcgacgaagt cggccgggaa cttggtctgc cagcgttccc aggcgtcttc gccggcttcg
     8761 gcgtcgcctt cggtgatgaa gtactcgacg ctgcgggccg acgccccgct gagggcggcg
     8821 gcgcgatcgg tctgtccctg ctcgggcaga tcgagcagca cctgcctgcc gcgctcaccg
     8881 ttgccggcga ggatcagcgc ttcgccgtac agccgctcgg tcgcgtgatc gtggcggcgc
     8941 gttagcggct ccagcagctt caccgctttt tccaaatcgc cggcccacca catcgcgatc
     9001 tgcgctgcac gtcgagcagc gcgcggttgc agttgcgaat caaccggcac gcggtcccac
     9061 agtttcgcca cctcgttcgg ctgccccgct tgcagcagcg cgcggtccaa ctccagcagc
     9121 gcatcgtacg ccgccgcggg ttgggggacc gtttggctct tcgccagctc atcgccggtg
     9181 ctcaacgccg cgtcgagttt ttgggcgcga agatgcagca gcgtcgcgcg catcagatcg
     9241 ttcggcggca tcttcgtcag gtcgtaatcc gcgatcacct tcgactgcgc cggcgggtct
     9301 tcgctcggcg gcgtggtgat gcggtcccac agccgactga tcggcaggcg gatcgtctgc
     9361 gtgtcgctgt tcggcccgac gcgcacggtc aggcgaaccg gcagcacatc ggcgtcgaga
     9421 aacacgtgat cgacatccgg ctgcgtcgcg gtttggccgt cgccgaaatc ccactcgtat
     9481 ttcagcggcg acgccgcgcg cgaatgggct ttgaagtgat agcgaaacga atagtggtcg
     9541 gcgacaaaac attcgccgat ttgctcggcg gcgaagtcgg ccagcagcga tcggccctgc
     9601 tgctcgagct ggcccacctg gcaaccgtag cagatgccga aggcttcgcg cgggatgagt
     9661 tcgaacttcc cgttgtccgg ccgctgccag gcgacggcga cccggccgtc gccgccggtg
     9721 ttcaggtgat acagcagtaa ttcgtgccgc ccgcgcgaca ggtgaaccgc ggcgttgtag
     9781 cgcacatccg gcgggccgat tggcgcgaac agcagcggct tgccgtcgat gaacagcgcg
     9841 gccgagtcgt caactgaaat cgcgaaggcg tattgaccgt cgatcggcgc gaagagcgaa
     9901 ccgatgaact tcgagatcca ctgctcctgc gggccgaaat agttgtagcc gatgaacggc
     9961 gtgggaatga cggcctgacc gagcgtgcgc gtgttgcggt tccagctgcg ctccagatcg
    10021 gcagcgctgc ccgcgccacc gccggaccaa gcgcgcgtct ccagcatcag ccccgcggtc
    10081 cacttcacgt cctcggtttc cttcgacgcc tccggcttcg gattgccgaa atagacggcg
    10141 tattttttga cgcccttctt cagcgtgaac gccaaacgaa tgcgatcgcc gggaccgtcc
    10201 tgcaggatgt gcgtggggac gagctttcca tcctcgcttg ccacgcgcac gtcgtcggcg
    10261 ttaggcaagg tgtgaccgtc ggaatagaat tccgccgccg ccagctggtc accgctgccg
    10321 tgctcggcgt cccaggtgac atcgatcgtc cgccgatatg gccaggcggc gtcgaaccag
    10381 gcgttggcgg gatttggaaa cgggagtagg aaccacagat gcacacagat gcacgcagat
    10441 aagagcgcgg ggcgtcgcgc gagccgcgcg atcgcacctt gacttggtgc gctccggtcc
    10501 cctcgcccgg tacaccggga gagggggtaa gagccacagg cgattgccct ggtcgcgcca
    10561 gcaatcgctg ctccgatctg tgtgcatccg tgtgcatctg tggttccaat acgcagcgat
    10621 gatgttaccc aaagcgatcc tgttcgacat ggacggcacg ttgacccagc cgtacatgga
    10681 ttacgaccgg atcaagcgcg acatgggcgt gggaccgggg ccgattttgg aatcgctggc
    10741 ggcgatggag ccgcagcaac gtcacgcggc cgaggcgatc cttcaccaac acgaagacgc
    10801 cgcggcgtcg gcatcgacgt tgaatcccgg ctgcgtggaa ttgctgacgt ggctgcgcga
    10861 aatcggcctg gagacggcgc tcgttacgcg caacagccgg cggagcgtgc gcacggtctt
    10921 cgatctgcac ggcctgcact ttgacgtgtg cgtgacgcgc gaagacggca aattcaaacc
    10981 cgaccccgcc ccgctgctgc tggcgtgcca gcggcttggc gtggagccgg ataaaacctg
    11041 gatggtcggc gacagttatc acgacataga cgccggcatc gccgcgaaga ttcgcacggt
    11101 gtgggtcagc catggcaagg cgaaggcgtt tgcgtccgag ccgtggcgca cggtgcggga
    11161 cttgctggag ttaacgcagg tgctgaaggc gtgtcttaag taatagcccg aggcggaaac
    11221 ccggggatcg gattgcgaac ggcgctattc gccaaacgac ccccggcctt ccggcctggg
    11281 ctatcacgga tggagtgctc atgcgagtga ttttgatctg cgttctgttg gtcatgtcaa
    11341 cgatggcgct ggcgcagccc gcgccgccgc tgatcaatcc cgtcggcgcc ggagccgata
    11401 gcgcgaccct gcgacccgac gcggggatgg atgaagtgct cgaagcgctg cacgagcgcg
    11461 ggcagaacct caaggagttc gtcgctgacg tcacgctgat cgacaacgat ttgattatgt
    11521 ccacgcagac gacgcgcacc gggaaagtgt gggcgcagtt caagccgggt ggcgatgcgc
    11581 ggttgcacgc gcgcttcgac aagcggatcg aggaaggcaa gccgccgcag aacgatctta
    11641 aagagtattc gctggacgac ggctggctgg tggatcgcga ctatcgcgcc aagaacgaag
    11701 tgcgccggca agtagcggcc ccgggcgaga aggtgaacct gttccagctg ggaaagggcc
    11761 cgttcccgct gccaatcgga caggaggtca agagcgttca cgagcagttc gacgtgacga
    11821 aggtcgagcc ggcgaaggac gatccaccgg gggcgattca cctacagctg acgccgaagg
    11881 agaagaccga tttagcgcgg aagtttcagg tgatcgacgt gtgggtggac ccggccacgc
    11941 ggatgccggt gcggatcaaa acgatcgatc acaaccagac gaccgagcgg cagacggatt
    12001 tcacgaacgt aaaggtgaac ccgacgccgg ggttgacgga tgcggatttc aagttgccgc
    12061 cgattgatca gtcgggctgg cagcggcatg aggaggcgct gcattaggct ttatcctgag
    12121 cgcaggcggt actccgccgg agtcgaagga tctggctggg agtcagtgta cgattgattt
    12181 ggagacggcg aggtagccgc gcgagtgatc cttcacttac ttctcggcta gatccttcga
    12241 ctgcgcacga ttacgtgctg cgctcaggat caactctaag ctgcgctcag gataaaccgg
    12301 cgaaattcgc cagcacctgc agccccacct tctggctctt ctccgggtga aactgcgtcg
    12361 ccatcacgtt gtcgcgccag atgctgctga cgaacggccg gccgtagtcg gtcgtcgtcg
    12421 cgatgatcga ttcgtcttcc ggcacgacgt gatagccgtg cacgaaatac acgctgcacc
    12481 cttctttcag atcgcgcagc agcggcgtgt tcgaccggcg attgagctgg ttccagccca
    12541 tgtgcggcac cttcaggccg agcgttgaat cgacgtcgaa gcgcacgcac ttgccgcgga
    12601 tgacgccgag cccgcggtgt tcgccgtctt cgtagccgac gtcgaagagc atctgcagtc
    12661 cgaggcagat gccgaggaag ggtttgccct tttcgatgtg gtggacgatc gggtcggcca
    12721 ggcgcttgtc gcgcagcgtc gcgacggcgt cttggaacgc gccgacgccg ggcaggacga
    12781 tcttgtcggc ccgctcgatc tgctcgggcg tggagatgat gtcggcggcg tggccgactt
    12841 cggccagggc tttctggacg gagcggaggt tggccatgcc gtagtcgatg atcgcgatgg
    12901 acattcgccg cattctaggc gggcaggggc aagcgcgcct aatcgatttc cgccgtcatc
    12961 gccgccggtt gtgcggccag taattcgtcc agcgtaaacc ggctgccgca ctcggggcag
    13021 cgcgactcgg ccaggcctgt caggttgtac ccgcagttgg ggcaggcgat ggcgctcccg
    13081 ccggcgcgtt tgatgcgcat cgttcgttcc gcggcgcttt cgcgccagat gaagaccgtg
    13141 aagatcaacc accacagcgg cgcgaggatg ctggagaagc agacggtgat ctcaccctcg
    13201 gttcgcgatg taccgtacgc cagcgtcgtc gcccccagcg ccaggagcaa caatgccccc
    13261 gctgccgcga cggtcagcca gatccgccgg ggcgtccagg cgatggactt accccagagc
    13321 agcagccaat aacccgcgac aaatccccac gtgacgatgc ccgcgccgat aaacatgccg
    13381 ttctcatgcg gataccccat cgactcttcg atggcgatcg ccgccagcat gtaaaccacc
    13441 gtcgccagcg gcagcatgaa gatcgccagc aggattcggg aaacgagctt ggacatgtgg
    13501 acacctcgag cgacatcccg cctgaaaact gaacccccag cgctccggca agtttcaacg
    13561 aatcagcctc ttgccgcgtt gcaattccgc gctgtcgccc ccacgcttgg cctttgacac
    13621 agaacgcact ggtcaggata atgcgggcaa accgggcggg ttacatgagt tgtcgcgccg
    13681 taccccgccg atctctcacc tttgagggta gttacggtat cccgagtcgt gccaaccggt
    13741 ggccactgcg ccgcgcgcgg gcttccagca ccacaatcag cgcgccgatc tcctccaatt
    13801 gttgattgcc gacaagagtt gtccagacgc gagttgttcc aaggtcgttg ccgagagtga
    13861 gaagacgaac tatgcgaaac gagcaccagg cgagcagcag cagcagcccc cggcgaagca
    13921 agagcaagac ccgccgcccc ggccggtcca caccgcggcg ggaccgatcg aacctgttcg
    13981 agatttttcc gctggaaaag cgcgtgctgc tgtccggcac gccgacgaca ttcgatcaca
    14041 cgttccgata cctggtcgcg ccggcgttaa cgccggcgcc cagcccgcat gtcaccggca
    14101 gcgcaacgcc ggtcggcctg aacgctgcgc agatccgcgg cgcatacggc gtggacaaca
    14161 tctccttcag caacggcgcg ctcggcaccg gcgctgggat caccatcgcg atcatcgatg
    14221 cctacgacac gccgagcatc attggtgatc tgcacacctt cga
//
LOCUS       DATIMH010000499         4313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5029843, whole genome shotgun sequence.
ACCESSION   DATIMH010000499 DATIMH010000000
VERSION     DATIMH010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5029843"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VLI90_08755"
     CDS             complement(<1..1238)
                     /locus_tag="VLI90_08755"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HSV14336.1"
                     /translation="MKIRKILPGGLFAAILLGLSFVAAGADATGDVATADTAGPKVRT
                     VAIFVKNRAKNVEDDKVTALEDMITSRITDSGFHVISREDVINSVKSFADAGPNVGDE
                     AEPGARLDKILSNNTSALRLAQNMNADYLLVASISTYGQDVQDFQEDAVRTRVTESTM
                     RVSYKLLDGVLGGSLTGDVVTAKVKDLEQPGQTVHRDMVNKLLDQASVKLADVLGAKV
                     AANKIRNAPENAKMATFSVDCSMTDLAIPEIIKSSDGQYQIMANSYQIKVMSASVELD
                     GVVVGTSPGEFTAGPGLHKIRVTRPGFEDYARTINIRDGLHLNVPMQLSGDGYARWRD
                     NIAFLQSMKDQAKLSDADTKRIEGIAQMFANSGFRVDTKSTSDVKSDYKGDVKINGNS
                     LWPTVQYGHDGRDGRDGRDGR"
     gene            1579..2415
                     /locus_tag="VLI90_08760"
     CDS             1579..2415
                     /locus_tag="VLI90_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14337.1"
                     /translation="MLLKKKARLTGVASLLGSEQIRRLEHEGDTYFAVADLVALLTES
                     ADPQQYFADLKAREPQLGQLVQTIDYTAADGSVTSLDVVSVDGAMRLIQSIPSPRAER
                     YKSWIAEAARQRFEEQQNPELAILRTRNLYESKGYSRPWVDKRLRGVSSRHELTREWS
                     KRGASESDDYRALTNELFDAAFGMDVAKYRAYKGLINRPSAHLRDHMTDLELVLTTLA
                     ESAAAILMRDRNSHGMEELTADARDAGEIVARTRFELEARRGRPAAYPGTVERASNDQ
                     AA"
     gene            2759..>4313
                     /locus_tag="VLI90_08765"
     CDS             2759..>4313
                     /locus_tag="VLI90_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HSV14338.1"
                     /translation="MAETTPPAGAPAPGMPADRIEDLQIERELQDSYLTYAMSTIMDR
                     ALPDVRDGLKPSQRRILVAMNDLNLTPRSKRIKCAKITGDTSGNYHPHGDAVIYPALV
                     RLAQPWALRYPLVDPQGNFGSTDGDPPAAQRYTEARMAEPTMDLLADLKLDTVDFQPN
                     YDERPGMEEPTVLPAKFPNLLVNGSTGIAVGMACNLLPHNAKEICNAIVKVIDNPDTS
                     LQELLEIVPGPDFPTGGIICGKSGIIDGYKNGRGRLTLRAKIHTEETKNGRTLVVIDE
                     IPYGIIRKSIVEAVAEAVKKDLINDLSAINDESGREHNCRIVIDLKRDADPNVIINQL
                     YQYTPCQITVSMINIALVNRQPRTMGLKEMIQHFIAHRKEVITRRTKFQLKKALQREH
                     ILEGTIFAVADIDEIIRLIRSSKTREEAIDKLMAQGFRITPDSAVLAKVPERIRFKHP
                     EIFALEPGVPMPPYRVSRAQAEAIGRLQLIQLVGLEIERLVNEYKEVVAEIEGYEYIL
                     NTPQAVIDIV"
BASE COUNT          863 a         1367 c         1312 g          771 t
ORIGIN      
        1 cggccgtcac gtccatcgcg cccgtcgcgg ccatcgtggc cgtactgcac ggtcggccac
       61 aagctgttgc cgttgatctt cacgtcgccc ttgtaatcgc tcttcacatc ggaggtggac
      121 ttggtgtcca cgcggaagcc gctgttggcg aacatctggg cgatgccttc gatgcgcttg
      181 gtgtcggcat cggacagctt cgcctggtcc ttcatgctct gcaggaaggc gatgttgtcg
      241 cgccagcggg cgtatccgtc gccggacagc tgcatcggaa cgttcaggtg cagcccgtcg
      301 cggatgttga tcgtgcgggc gtaatcttca aagcccggac gagtcacgcg gatcttgtgc
      361 agccccggcc cggcagtgaa ttcgccgggc gacgtgccga cgacaacccc atcgagttcg
      421 acgctcgcgg acatgacctt gatctgatag ctgttggcca tgatctgata ctggccgtcg
      481 ctgctcttga tgatctcggg gatggccagg tcggtcatgc tgcaatcgac gctgaaggtg
      541 gccatcttcg cgttttccgg cgcgttgcgg atcttgttgg ccgccacctt cgcgcccagc
      601 acgtcggcca gcttcaccga agcctggtcg agcagcttgt tgaccatgtc gcgatgcacg
      661 gtctgcccgg gttgttccag gtccttcacc ttggcggtga cgacgtcgcc ggtgagcgac
      721 ccgcccaaca cgccgtcgag cagcttatag ctgacgcgca tcgtcgattc ggtcacgcgg
      781 gtgcgcaccg cgtcttcctg gaaatcctgc acgtcctgcc cgtacgtcga gatcgacgcg
      841 acgagcaaat agtcggcgtt catgttctgg gccaggcgaa gggccgacgt gttgttcgaa
      901 aggatcttgt cgagccgcgc gcccggctcg gcttcgtcgc cgacgttcgg cccggcgtcg
      961 gcgaaggact tcaccgagtt gatcacgtct tcgcgactga tgacgtggaa gccggaatcg
     1021 gtgatccggc tggtgatcat gtcttccagc gcggtgacct tgtcgtcctc cacgttcttg
     1081 gcgcggttct tcacgaagat ggcgacggtg cgcaccttcg gcccggccgt gtctgcggtc
     1141 gcgacgtcgc cagtcgcatc cgcgccagcg gcaacaaatg acaagcccag caatattgcc
     1201 gcgaacaacc caccggggag tatttttctg atcttcatct tggacctcta atcggatgac
     1261 cgtgggccgc cgcgatagcc gaacgcagct ccccgctgga catgacgcgg ggcgagtcta
     1321 tctctgctcc atttcggatt caaacattta tctgcaacgg acatctgaat cggccacgca
     1381 ccgatcgaat tgtccgcaga cttcggcccg tcatcggtac cgtctcggca gacaccgctt
     1441 gcgcgcgttg gttttgcgcc ggttcttata acgcttgcgg ttgctaactt ggggaatttt
     1501 tctccccaaa caaacgccga actttgctat actgccggac gtactaatcc atggaattag
     1561 cagaagttgg agcaaaaaat gctgttgaag aaaaaagcac gtttaacggg tgtcgccagc
     1621 ctgctgggga gcgagcaaat ccgccggttg gagcatgagg gcgacacgta cttcgccgta
     1681 gcggacctcg tcgcgctgct gacggaatcg gcggatccgc agcaatactt cgctgatctc
     1741 aaggcgcgcg agccacagct gggacagctc gttcaaacca tcgattacac cgccgccgat
     1801 ggcagcgtga cgagcttgga tgtcgtgtcg gtcgatggcg cgatgcggtt gattcagtcg
     1861 atcccgtcgc cgcgcgccga gcgatataag agctggatcg ccgaagcggc gcgccagcgg
     1921 tttgaagagc agcagaaccc ggagctggcg atcctgcgca cgcgcaacct gtacgaaagc
     1981 aaaggctaca gccggccgtg ggtggacaaa cgactccgcg gcgtgagctc gcggcacgag
     2041 ctgacgcgcg agtggtccaa gcgcggggcg agcgaaagcg acgactatcg cgcgctgacg
     2101 aacgagctgt tcgacgccgc cttcggcatg gatgtggcga agtatcgcgc gtacaagggg
     2161 ctgatcaatc ggccgagtgc ccacctgcgc gatcacatga cggacctgga gctggtgctg
     2221 acgacgctgg ccgaatcggc cgcggcgatt ctaatgcgcg accgcaacag ccacggcatg
     2281 gaggagctga cggcggatgc gcgcgatgcg ggcgagatcg tcgcgcgaac gcgattcgag
     2341 ttggaagcac gaaggggtcg cccggcggcg tatccaggca cagtcgaacg cgcgtcaaac
     2401 gaccaggcgg cctaatcccc tctggtcccc tcgcccatct actcatggga gagggttagg
     2461 gtgagggtga tttcaaacgt cgagcggctc ttgaaggaca aaaatcaccc tcacccgaac
     2521 cctctcccgg cgtatcgggg gggccagaag gcggattctc ggcgttggaa aatcgcgctc
     2581 gaacggttcg cgtcgcgagt cattttccag tacaatttcc cccttccgcc ggccgtgtgc
     2641 acggacgtat ccggcctata atttcaggga aatttccggg gctaacggag ttgtattcgc
     2701 cccgcggaaa cggcggctga aaccgatctg acccacgaac gcttaacgac gaaaaaccat
     2761 ggccgaaacc accccacccg ccggcgcacc ggcaccgggc atgcccgctg accgtatcga
     2821 agacttgcag atcgaacgcg aactgcagga cagctatctc acgtacgcca tgagcaccat
     2881 catggaccgg gccctgcccg atgtccgcga tgggctcaaa ccgtcgcagc ggcgaatcct
     2941 cgtcgcgatg aacgacctga acctcacacc gcgcagcaag cgcatcaagt gcgccaagat
     3001 caccggcgac accagcggta actatcaccc gcacggcgac gcggtcatct accccgcgct
     3061 cgttcgcctc gcgcagccgt gggcattgcg ttaccctctg gtcgatccgc agggcaactt
     3121 cggcagcacc gacggcgatc ccccggccgc ccagcgttac accgaagccc gcatggccga
     3181 gccgacgatg gatctgctgg ccgacctgaa gctcgacacc gtcgatttcc aacccaacta
     3241 cgacgaacgc cccggcatgg aagagccgac cgttctgccg gcgaagtttc cgaatttgct
     3301 ggtgaacgga tcgaccggca tcgccgtggg catggcgtgc aatctgctgc cgcacaacgc
     3361 gaaggaaatc tgcaacgcga tcgtcaaggt catcgacaac cccgacacct cgctgcagga
     3421 gctactggag atcgtcccgg gccccgactt ccccaccggt gggatcatct gcggcaagag
     3481 cggcattatc gacggttaca agaacggccg cggccggctg acgctgcgcg ccaagatcca
     3541 caccgaggaa accaagaacg gccgcacgct cgtcgtcatc gacgagatcc cctacggcat
     3601 catccgcaag tcgatcgtgg aagcggtggc cgaggcggtg aagaaggacc tgatcaacga
     3661 cctgagcgcg atcaacgacg aatccggccg cgagcacaac tgccgcatcg tcatcgacct
     3721 gaagcgcgat gcggatccga atgtcatcat caatcagctg taccaataca cgccgtgcca
     3781 gatcaccgtg tcgatgatca acatcgcgct ggtgaaccgc cagccccgca caatggggct
     3841 gaaggagatg atccagcact tcatcgccca ccgcaaagag gtcatcaccc ggcggacgaa
     3901 gttccagctg aaaaaggccc tgcagcgcga gcacattctg gaaggcacga tcttcgccgt
     3961 cgccgatatc gacgaaatca tccgcctgat ccgcagcagc aagacgcgcg aggaagccat
     4021 cgacaaactg atggcccagg gcttccgcat cacgccggac agcgccgtgc tggccaaggt
     4081 gccggagcgc atcaggttca agcatccgga gatcttcgcg ctggaacccg gcgtgccgat
     4141 gccgccgtat cgcgtgtcgc gcgcacaggc cgaagcgatc ggccgcctgc agctcatcca
     4201 gttggtcggc ctggagatcg agcgcctggt aaacgagtac aaggaggtcg tcgccgagat
     4261 cgaggggtac gagtacatcc tgaacacgcc gcaggccgtg atcgacattg tcc
//
LOCUS       DATIMH010000500         5491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5605733, whole genome shotgun sequence.
ACCESSION   DATIMH010000500 DATIMH010000000
VERSION     DATIMH010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5605733"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VLI90_08770"
     CDS             <1..645
                     /locus_tag="VLI90_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14339.1"
                     /translation="KLIVVEGTDGCGRSTQAVMLKQWLEVQGYGVMETGWTRSKLVGQ
                     AITDAKQGHSLNRLTYCMMYATDLADRMEYQIIPALRSGFIVLADRYVYTAIARGIVR
                     GADKQWLRDLYGFAVVPDLVFYLKLGVHDLVPRVLQAGKMNYWESGMDMNYGDDLYDS
                     FMAYQGALVDQFDLMAEEEGFVTVDARSSPNAIQAELRRVVSRYLHDVPTAGMV"
     gene            871..1353
                     /locus_tag="VLI90_08775"
     CDS             871..1353
                     /locus_tag="VLI90_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSV14340.1"
                     /translation="MDDSTTTIDLTVNGQKHTITTHPQRPLLDVLREDLHLTGTHFGC
                     GQGDCGACGVLLDNQRIFSCSTPISDVAGKSVTTIEGLAQGEKLHPVQQAFLDEGAFQ
                     CAYCTSGMIVAAIALLREKPKPSDDEIRDGMNGNICRCCSHAKIMSAIHRAIAAGVTP
                     "
     gene            1350..3440
                     /locus_tag="VLI90_08780"
     CDS             1350..3440
                     /locus_tag="VLI90_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSV14341.1"
                     /translation="MIAATRRTFLQTLGAGILISVTAPSLFAQQRRRGGGGMGGAGAR
                     TVAARIHIGNDGVITVMTGKVECGQGARAELTQAAAEELRVAVDQIQLIMADTALVPD
                     DGITAGSGTTPRTVPAIRQGAAAAREMLIDAGAKRLNVPRAEVEARDGKIVHAPSNRS
                     VSYAELARDDLTKSMATTQPSDIAVTPVKQWQVMGTPTPRPNRRDLVTGGHQYPSDIA
                     RPGMMYGKVLRPAGFHATLTGIDLGPAQALKDVTVVRDGDFIGVVAPTSFAARGAIEL
                     LEKSAKWDVPPQIGSAQLYEHLQQHARGDGLQNPFADELAKSAKTLKQTYHVAYIQHA
                     PMEPRAAVAEWNDGKLTVWTGTQNPFGVRSELARAFSIPAESIRVIVPDFGGGFGGKH
                     SGEAAVEAARLAKGAGKPVSLRWSREEEFTWAYFRPAAVIVAEAGLDADNKLTSWHFV
                     NINSGPSAVKTPYRVVKNNCQFVQSDPPLRHGSYRGLAGTANHFARESFMDELSTAAG
                     MDPLAFRLAHIDDPRLRAVLETAAEQFGWADRVKQKDENIGVGLACGTEKGSYLAACV
                     EAAVDRSKGTIAVRRVVQAFECGAIVNPSNLLSQVQGAIMMGIGPALREAIRFENGKV
                     LTTAFSEYEVPRFADLPQLEVHLIDRPDLPSAGAGETPIMAVAPAIANAVCHATAVRV
                     REMPIVLPRTLSPR"
     gene            complement(3590..4234)
                     /locus_tag="VLI90_08785"
     CDS             complement(3590..4234)
                     /locus_tag="VLI90_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14342.1"
                     /translation="MLRMILYRGASILLAVGTCWWLTGNVGAMTALAIWVGASAAMCA
                     MAAVLLHRQAPGRIGVVNYAAGFVLPWGYRIGNGRLTAIAITSAIIWALLGAAVVTLR
                     IPASSAAHVDNTHAVLRALLLMSWIIDGGALLYLLSVMSRSPGLVRSLLKVTAIVAAM
                     LIASVALTLFSHPGSGTMMPLALAISGGPPLVVGSAYGLFLLVMLTVGRNARWN"
     gene            complement(4397..5170)
                     /gene="hisF"
                     /locus_tag="VLI90_08790"
     CDS             complement(4397..5170)
                     /gene="hisF"
                     /locus_tag="VLI90_08790"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HSV14343.1"
                     /translation="MLTKRIIPCLDVDRGRVVKGVKFFDHLDAGDPVEQAKRYQNDGA
                     DELVFYDITASHEGRQIMADLVAKVMDNVFMPLTVGGGIRTLDDATRLIQAGAEKVSL
                     NSAAVRNPKLIAEVARKFGRCATVLGLDANRVKGADGNEVWEVFVNGGRVGTGIKVVD
                     WVRQAEDLGAGEIVLNVMNADGTVAGYDLEMTAAVSDAVKIPVVASGGAGSPKHMLDV
                     LTIGHADAALAASIFHFNTYSIGQVKDYLNGQGVPVRLS"
     gene            complement(5264..5336)
                     /locus_tag="VLI90_08795"
     tRNA            complement(5264..5336)
                     /locus_tag="VLI90_08795"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5301..5303),aa:His,seq:gtg)
BASE COUNT         1033 a         1776 c         1755 g          927 t
ORIGIN      
        1 aagctgatcg tcgtcgaagg caccgacggc tgcgggcgat caacccaagc cgtcatgctc
       61 aagcagtggc tcgaggtgca aggctacggc gtgatggaaa ccggctggac gcgcagcaaa
      121 ctggtcggcc aggccatcac cgacgccaag cagggtcact cgctgaaccg cctgacgtac
      181 tgcatgatgt acgcgaccga tctggctgac cggatggagt atcagatcat ccccgccttg
      241 cgcagcgggt tcatcgtgct ggccgaccgc tacgtctaca ccgccatcgc ccgcgggatc
      301 gtccgcggcg ccgataagca atggctgcgc gatctgtacg gcttcgccgt cgtccccgac
      361 ctggtcttct atctgaagct cggcgtgcac gatctcgttc cgcgcgtgct gcaagccggc
      421 aagatgaact attgggaaag cggcatggac atgaactacg gcgacgacct gtacgacagc
      481 ttcatggcgt accaaggcgc gcttgtcgat cagttcgacc tgatggcgga ggaagaagga
      541 ttcgtgacag tcgatgcccg ctcgtccccc aatgcgatcc aagccgagct ccgccgcgtc
      601 gtcagccgct atctgcacga cgtcccgacc gccggaatgg tgtgaggcgt cgaatgcagc
      661 atgttgaacg gagaatgaac gtcacgagga gccgcgcaca aagcgtactc gcgcagtaag
      721 cggacgtcgc acatgcccgc ttactgcgcg agtacgctcc gtgcgcggct ccttcccatc
      781 ggatgatctt cccttctgga ttctgtattc aagaattccc tcgccacacc cgccgctcgt
      841 tgaggtataa acgttcacga ggtgcccatc gtggacgatt cgacaacgac catcgatctg
      901 accgtcaacg gccaaaagca cacgatcaca acccacccgc agcggccgct gctggacgtg
      961 ctgcgcgaag acctgcacct gaccggcacg cacttcggct gcggccaggg cgactgcggc
     1021 gcgtgcggcg tgctgctcga caatcagcgt atcttttcct gcagcacgcc gatcagcgat
     1081 gtcgcgggca agtctgtcac gacgatcgaa ggcttggcgc agggcgagaa gctgcacccg
     1141 gtgcagcagg catttctcga cgaaggcgcg ttccagtgcg cgtattgcac cagcggcatg
     1201 atcgtggcgg ccatcgcgct gctgcgcgag aagcccaaac caagcgatga cgaaatccgc
     1261 gatggcatga acggcaacat ctgccggtgc tgcagtcatg cgaagatcat gagcgcgatc
     1321 catcgcgcga tcgccgcggg ggtgacgcca tgatcgctgc cacccgtcga acatttttgc
     1381 agacgctcgg cgcgggcatt cttatctcgg tcaccgcgcc gtccctcttc gcccaacaac
     1441 gccgccgcgg cggcggtgga atgggtggcg ccggcgcgcg aacggtcgcc gcgagaattc
     1501 acatcggtaa cgacggcgtg atcaccgtca tgaccggcaa ggtcgaatgc ggccaaggcg
     1561 cgcgggccga gctaacgcag gcggcggcag aggaactgcg ggtggccgtc gatcagatcc
     1621 agctgatcat ggccgacacc gcactcgtac cggatgacgg catcaccgcc ggcagcggga
     1681 cgacaccgcg aacggtgccg gcgatccgcc aaggcgccgc ggccgcgcgc gagatgctca
     1741 tcgacgccgg ggcaaagcgc ttgaacgtgc cgcgcgccga ggtcgaagcg cgcgatggca
     1801 agatcgttca cgcgcccagc aatcgatcgg tttcatacgc tgaactcgcg cgcgacgatc
     1861 tgacgaagtc gatggcgacg acgcagccga gcgacatcgc ggttacgccg gtgaaacagt
     1921 ggcaggtgat gggcacgccg acgccccggc cgaaccggcg cgatctggtc accggcgggc
     1981 atcaatatcc atccgacatc gcgcggcccg ggatgatgta cggcaaggtg ctgcggccgg
     2041 cgggatttca tgcgacgctg acgggcatcg atcttggtcc cgcgcaggcg ctgaaggacg
     2101 tgaccgtcgt tcgcgacggc gacttcatcg gcgtcgttgc cccgaccagc ttcgcggcgc
     2161 gcggcgctat cgaattgctg gagaagtccg cgaagtggga cgtcccgccg cagatcggca
     2221 gcgcgcagtt gtacgaacat ctgcaacagc atgcccgcgg cgatggtttg cagaatccgt
     2281 tcgccgatga attggcgaag tccgccaaga cgctgaagca aacctaccac gtcgcgtaca
     2341 tccagcacgc cccgatggaa ccgcgagcag cggtcgcgga gtggaatgac ggcaagctca
     2401 cggtctggac cggcacgcag aatccgtttg gcgtgcgatc ggaactggcc cgcgcgttca
     2461 gcattccagc agaatcgatc cgcgtgatcg tgccggattt tggcggtggg ttcggcggga
     2521 agcacagcgg cgaagccgcc gtcgaagcgg ctcgtctcgc caagggcgcc ggcaagccgg
     2581 tgtcgctgcg ctggtcgcgc gaagaggaat tcacctgggc ctacttccgc cccgccgccg
     2641 tcatcgtcgc cgaggcgggg ctcgacgcgg acaacaagct taccagctgg cacttcgtca
     2701 acatcaactc cggcccgtcg gcggtgaaga cgccctaccg cgtggtgaag aacaattgcc
     2761 agttcgtgca gtccgacccg ccgctgcggc acggctccta ccgcgggctg gccggcacgg
     2821 cgaatcattt cgcgcgcgaa agttttatgg atgaactgtc gacggccgcg gggatggacc
     2881 cgctggcgtt tcggttggcg catatcgacg atccgcgctt gcgcgccgtc ctcgaaaccg
     2941 ccgccgaaca attcggctgg gccgatcgcg tcaagcagaa ggacgagaac attggcgttg
     3001 gcttggcgtg cggcaccgag aaaggttcgt acctcgccgc ctgcgtcgag gcggcggtgg
     3061 atcgatccaa gggcacgatc gccgtccgcc gggtggttca ggcgttcgag tgcggcgcga
     3121 tcgtcaatcc atcgaacctg ctgtcgcagg tgcaaggggc gatcatgatg ggcatcgggc
     3181 ccgcgctgcg cgaggcgatt cgattcgaaa atggcaaagt gctgacgacg gcgttcagcg
     3241 agtacgaggt gccgcgtttt gcagacctgc cgcagctgga ggtgcatctg atcgaccgac
     3301 ccgacctgcc ctccgccggc gccggtgaaa cgccgatcat ggccgtcgcg ccggcgatcg
     3361 cgaatgcggt gtgtcacgcg actgccgtgc gcgtgcgcga gatgccgatc gtgctgccga
     3421 ggacgctgtc gccgaggtga tggatctata gcgcgcgtat ttctcacacg ccgggttcgg
     3481 agcgtattcg cgacgacccg gatctttcgc gcgacgagtc cgggtcgtcg gagtacctcc
     3541 gaacccggcg tggcttgctt ctccaattac tttgccaagg tacatgaact cagttccacc
     3601 gcgcgttccg gcccaccgtc agcatcacga gcagaaacaa gccataggcg ctgccgacga
     3661 ccagcggcgg gccgccggaa atcgccagcg cgagcggcat catcgtgccg ctgccggggt
     3721 ggctgaacag cgtcagcgcg acgctggcga tgagcatcgc ggcgacgatg gcggtcactt
     3781 tcagcagcga tcgcaccagg ccggggctgc gtgacatgac gctcagcagg tacaacagcg
     3841 ccccgccgtc gatgatccag gacatcagca gcagcgcccg cagcaccgcg tgcgtgttat
     3901 cgacatgcgc ggccgatgat gcgggaatgc gcagcgtcac tacggccgca ccgagcaacg
     3961 cccagatgat cgccgaggtg atcgcgatgg cggtcaatcg accgttgccg atgcggtatc
     4021 cccacggcag cacgaacccg gcagcgtagt tgacgacgcc aatgcgcccg ggcgcttggc
     4081 ggtgcaggag caccgcggcc atcgcgcaca tcgccgccga tgcgccgacc cagattgcca
     4141 aggcggtcat agcgccgacg ttcccggtca accaccagca cgtgccgacc gccagcagga
     4201 ttgatgcgcc acggtagagg atcattcgca gcacaagtga agcgcctgta cgcgttcgga
     4261 ttacttgtgc gcgctttccc cctcgcccgg tactccggga gaggggacca gagcgcacgt
     4321 ctttagccca attcgtatta caccatcgcc ggagtattcc tgcgcgcgat tgtcagccgg
     4381 tcgaaatgaa gaaatgctac gacaaccgaa ccggcacgcc ctggccgttc aagtagtctt
     4441 tcacttgccc gatgctgtag gtgttgaagt gaaagatgct cgccgccaac gcggcgtcgg
     4501 cgtggccgat ggtcagtaca tcgagcatgt gcttgggcga gcccgccccg ccgctggcga
     4561 cgaccgggat tttcaccgcg tcgctgacgg cggcggtcat ttccaagtcg tatcctgcga
     4621 ccgtgccgtc ggcgttcatc acgttcagga cgatctcgcc ggcgcccaag tcttccgcct
     4681 ggcggaccca atccacgacc ttgatgccgg tgccgacccg gccgccgttg acgaagactt
     4741 cccagacttc gttgccgtcg gcgcctttga cgcggttggc gtcgaggccc agcacggtcg
     4801 cgcagcggcc gaacttgcgg gcgacctcgg cgatcaactt tggattgcgc acggccgcgg
     4861 agttgaggct gactttttcc gcgccagctt gaatcaggcg cgtcgcatcg tccagcgtgc
     4921 gaatcccacc gccgacggtc agcggcatga agacgttgtc catcaccttg gcgaccaagt
     4981 cagccatgat ctgccggccc tcgtgcgacg cggtgatgtc ataaaacacc agctcgtccg
     5041 ccccgtcgtt ctggtagcgc ttggcctgct ccacgggatc gccggcgtcc aggtggtcga
     5101 agaacttcac gcctttgaca acgcggccgc gatcgacgtc caggcagggg atgattcgct
     5161 tggtgagcat aaacgaaaag ttgctagttg ctggttgttc gttgctagtt ctgccaatcc
     5221 gccactagca accagcaacc aacaactagc aactgattcc aagggtgacc gacgggactc
     5281 gaacccgcga cgtccaggac cacaacctgg cgctctacca actgagctac ggccaccgac
     5341 ttcggcgtcg aacaggactc attctttacc gcaatagggg cggtagtcaa tacgcgcgag
     5401 cgacgctgga gcgttgcagg cccccccacg ccgggttcgg aggtactccg acgacccgga
     5461 ttcttctcgc gcgccgcaga atcgggtcgt c
//
LOCUS       DATIMH010000501         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3293955, whole genome shotgun sequence.
ACCESSION   DATIMH010000501 DATIMH010000000
VERSION     DATIMH010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3293955"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VLI90_08800"
     CDS             <1..744
                     /locus_tag="VLI90_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSV14344.1"
                     /translation="QFIRYVPMVAERGARVVLLSHQPKLLPLLKQIPGVARIITEAWE
                     QPQYDVQCSLLALPRLFKTTVATIPRGDAYLKADPALTATWADRLIALNKPGKRVGIV
                     WAGNPDHRNDRNRSMPLEALLPLGEMVGITLYSLQKGPAAAQLAASPALGAKLIDIAP
                     QLNDFADTAAAIVNLDLIISVDTSVAHVAGAMGKPVWLLLPLSPDWRWMLDRADSPWY
                     PSMRLFRQRRLGDWAGVAADVAQAIDQLD"
     gene            966..2006
                     /locus_tag="VLI90_08805"
     CDS             966..2006
                     /locus_tag="VLI90_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14345.1"
                     /translation="MPKPRLHLFVLLAAFSAALGSSHALAGDEPYKLAQSVHIGGEGS
                     WDYVTLSADGSRLYVPRTTHTQVLDTATWKVVADIPGTKRSHGVALVPSVARGFVSDG
                     TDASVQIFDLKTNEVLGTIKAQPDADGIIYDAFSNHVFVVSGDNGVLIPIKPDVDPKS
                     GSAEAAIDLGGKPEFLVSDNKGKLYINLADKAQMAVVDTQTMKVIDHWPTAPGTDPTG
                     LSMDREKGHLFVGCRNQKLLIMNAADGKILGDLPIGVRVDATVVDKPYALASCADGTL
                     SVASETSPGKYEVVQVVKTAAGARTMAVDNATGRIYLPAADMTPADPANPKARPKAVP
                     GSFKIIVVERAK"
     gene            complement(2014..>2340)
                     /locus_tag="VLI90_08810"
     CDS             complement(2014..>2340)
                     /locus_tag="VLI90_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV14346.1"
                     /translation="VIPYFSLAAGFLTGKYRSATDAEGKARAPRVMKYLNDRGFAVLA
                     ALDDVARDLKSTPARVALGWLMARPSITAAIASATNLDQLKDLLASTELKLDASYIDR
                     LNRASQ"
BASE COUNT          427 a          683 c          810 g          420 t
ORIGIN      
        1 cagttcatcc gatatgtgcc aatggtcgcc gagcgcggcg cgcgcgtcgt gctgctgtcg
       61 catcaaccaa aattgctgcc gctgctgaag caaattcccg gcgtcgcgcg catcatcact
      121 gaagcgtggg agcagccgca gtacgacgtg caatgttcgc tgttggcgct gccgcggttg
      181 ttcaagacga cggtcgcgac gattccgcgc ggcgatgctt atctgaaggc ggaccccgcg
      241 ctgaccgcga cgtgggcgga tcggttgatc gcgctgaaca agccgggcaa acgtgtcggc
      301 atcgtgtggg cgggcaaccc cgatcatcga aacgatcgca accgatcgat gccgctggag
      361 gcgctgttgc cgctcggcga aatggtgggc atcacgctgt acagcctgca gaaaggtccc
      421 gccgccgctc agctggcggc gtcgccggcg ctgggcgcga agctcatcga catcgccccg
      481 cagctgaacg acttcgccga caccgcggcg gcgatcgtga atctggatct gatcatcagc
      541 gtggacacat ccgtcgcgca cgtggccggc gcgatgggca agccggtgtg gctgctgctg
      601 ccgctgtcgc ccgattggcg ctggatgctg gatcgagcgg attcgccgtg gtacccgagc
      661 atgcgattgt tccgccagcg caggctcggc gactgggcgg gggtggcggc ggatgtcgcg
      721 caggcaatcg atcaactcga ttgagtctga gaagccccca cgccgggttc ggaggcctgt
      781 cctgagcgaa gtcgaaggat actcgacgat ccggatgctt ttcgcgcgcc ataagaatcc
      841 gggtcgtcgg agtacctccg aacccggcgt ggggaggttt tgaggatcat gacaccgtca
      901 tgtgacgtaa cgtaggttcc agccgcttca tgcacgcgcg tgcgcgtagg aaaaggactc
      961 gactcatgcc gaaaccacgt cttcatctgt tcgtattgct cgccgcattc tcggcggcgt
     1021 tgggttcatc gcatgccctc gcgggtgatg agccatacaa gctggctcaa tccgtccata
     1081 tcggcggcga gggcagctgg gattacgtca cgcttagcgc cgacggcagc cggttgtatg
     1141 tgccgcgcac cacgcacacg caggtgctcg atacggcgac gtggaaggtc gtcgccgaca
     1201 tccccggcac caagcgatcg catggcgtgg cactggtgcc ttcggtggcc cgcgggttcg
     1261 tcagcgatgg cactgatgcg tcggtgcaga tcttcgatct gaagacgaac gaggtgctcg
     1321 gcacgatcaa ggcgcagccg gatgccgatg gcatcatcta cgacgcgttc agcaatcacg
     1381 tcttcgtcgt cagcggcgac aatggggtgc tgatcccgat caagccggac gtggatccga
     1441 agtccggcag tgctgaggcg gcgatcgatc tgggcggtaa gccggagttt ctcgtctccg
     1501 acaacaaagg aaagctgtac atcaacctgg ccgacaaagc gcagatggcg gtcgtcgata
     1561 cgcagacgat gaaggtgatc gatcactggc cgaccgcgcc gggcacagac ccgacggggt
     1621 tgtcgatgga tcgcgagaag gggcatctgt ttgtcggctg tcgcaaccaa aagctgctca
     1681 tcatgaacgc cgccgatggg aagatcctgg gcgacctgcc gattggcgtg cgcgtggacg
     1741 cgacggtggt ggacaagccg tacgcgctgg cgagctgcgc cgacgggacg ctgagcgtgg
     1801 ccagcgaaac gtcgccgggg aagtacgagg tggtgcaggt ggtgaagacg gcggcggggg
     1861 cgcggaccat ggcggtggat aacgctactg gaagaattta tttgccggcg gcagacatga
     1921 cgccggccga tccggcgaat ccgaaagcgc ggccgaaggc cgtgccgggg tcgtttaaga
     1981 tcatcgtggt ggagcgggcg aaatgatgcc cggctattgg ctggcgcggt tgaggcggtc
     2041 gatgtatgac gcgtcgagtt tcagttcggt ggatgcgagg agatctttga gctgatcgag
     2101 gttggtagcg ctggcgatcg ccgcggtgat gcttggccgc gccatcagcc accccagcgc
     2161 gacccgcgcc ggcgttgact tcagatcgcg cgccacgtca tccagcgctg ccagcacagc
     2221 gaagccgcgg tcgttcaggt acttcatcac gcgcggcgcg cgggccttgc cttcggcatc
     2281 cgtggccgag cgatattttc ccgtcaggaa tccggccgca agggaaaagt aggggatcac
//
LOCUS       DATIMH010000502         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1563257, whole genome shotgun sequence.
ACCESSION   DATIMH010000502 DATIMH010000000
VERSION     DATIMH010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563257"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..383
                     /locus_tag="VLI90_08815"
     CDS             69..383
                     /locus_tag="VLI90_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HSV14347.1"
                     /translation="MITIPPVLAQGTVDDNTGLGIIAWIVVGLIAGFLGSKIVNKRGE
                     GIVVDIILGLVGSIVGGFVFHLLGIHRNGSFILSVIVATIGAVLVLLIYHKLIRSGAT
                     RA"
     gene            complement(425..1879)
                     /locus_tag="VLI90_08820"
     CDS             complement(425..1879)
                     /locus_tag="VLI90_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSV14348.1"
                     /translation="MTDRTKKWLKFTVRWGIAAFGVWWVLSNINFTDRVMVLDPATNR
                     PRYVAAINAQEDAAEFIIAPKGEEPRRIRRDELWVRPDRAAVSIRLSPGEPPQKRKLL
                     AIKPGGATSKGRSIRELLVEDPATHRGVVVLPDQLVGARGLDVASPYVEIGLTRMVRE
                     ADRSFLIAAICILPIGYVLTSIRWFFLLRGLGVQIGLGRTLVINMVGAFYNSFMPGTT
                     GGDVIKAYYAAKHTPLRTRAVMTVLVDRVIGLLALIIVGGSMAAYQWEIEDCRRVAIG
                     SAVILVGVGLGAIIFYNRTLRRLSGLSFILSRLPMQKQVHKAVEAMEVYGHRPLPVVA
                     ATLVSFPVHAMSILSASFAGMAFRLPLGLFYYWVVVPVVALVGAIPISPQGAGVMEFF
                     AVQLTKRQGATISQAFILTMSIRLLAMFWNLVAGLFVLRGGYHSPTVKEQEEMESDDS
                     SAAASDATAAKTSEAMTSSPPPPALRGGAGEGAA"
     gene            1973..>2164
                     /locus_tag="VLI90_08825"
     CDS             1973..>2164
                     /locus_tag="VLI90_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14349.1"
                     /translation="MQIGPVRATAAAVILFVGGCAAIPIATVGTVAGLAASTVDTGAD
                     LYKMGKLDAADLATFDDVVA"
BASE COUNT          386 a          686 c          725 g          367 t
ORIGIN      
        1 ctatgcggat gtaccgcgcc ctgcaatgct gcacccctca ttcaccagga agttctatga
       61 tgaacgcatt gatcaccatc ccccccgtcc ttgcccaggg caccgtcgat gacaacaccg
      121 gtctgggcat catcgcctgg atcgtcgtcg gcctgatcgc cggattcctc ggcagcaaga
      181 tcgtcaacaa acgcggcgaa ggcatcgtcg tcgacatcat cctgggcctg gtcggctcga
      241 ttgtcggtgg cttcgtcttc catctgctgg gcatccaccg caacggctca ttcatcctga
      301 gcgtgatcgt cgccacgatc ggcgccgtgc tggtgctttt gatctaccac aagttgatcc
      361 gatctggcgc gacccgcgcc tgaatcaccc ccgcgtggtc gtcgaacttg accgcggggc
      421 gacgtcaggc tgcgccttcg ccggcgccgc cgcgaagcgc tgggggtggg ggcgaagagg
      481 tcatcgcttc ggaagtcttc gcggcggttg cgtcggacgc tgcggcgctg gaatcgtctg
      541 actccatctc ctcctgctct ttcactgtcg gcgagtgata cccgccgcga aggacgaaca
      601 gaccggccac caggttccag aacatggcca gcaggcggat ggacatcgtg aggatgaacg
      661 cctggctgat ggtggcgccc tggcgcttgg tgagttggac ggcgaagaat tccatcacgc
      721 cggcgccttg cgggctgatg gggatcgcgc cgaccagcgc gacgacgggc acgacgaccc
      781 agtagtagaa cagccccagg ggcaggcgga acgccatgcc tgcgaagctg gccgagagga
      841 tcgacatcgc gtggacgggg aaggagacga gcgtcgcggc gacgacgggg agcgggcggt
      901 ggccgtagac ctccatcgct tcgacggcct tatgcacctg cttctgcatc ggcaggcggc
      961 tcaggatgaa agacagaccg gacaagcgac gcagcgtgcg gttgtagaag atgatggcgc
     1021 ccagcccgac accgacgagg atgacggccg agccgatcgc gacgcggcgg caatcttcaa
     1081 tctcccactg ataggctgcc atgctgccgc cgacgatgat cagggcgagc aggccaatca
     1141 cgcggtcgac cagcacggtc atgaccgcgc gggtgcggag cggcgtgtgc tttgcggcgt
     1201 agtaggcttt gatcacgtcg ccaccggtcg tgccgggcat gaaggagttg tagaacgccc
     1261 ccaccatgtt aatgaccagc gtccgcccca ggccgatctg cacgcccagc ccgcgcagca
     1321 ggaagaacca gcgaatgctc gtcagcacat acccgatcgg caggatgcag atcgctgcga
     1381 tcaggaacga gcggtcggcc tcgcggacca tccgtgtcag tccgatctcg acgtaggggg
     1441 aggcgacgtc cagaccgcgg gcgccgacga gttgatcggg aaggacgaca acaccgcggt
     1501 gggtggcggg atcttcaacg agcaactcgc ggatggatcg acccttcgac gtcgcgccgc
     1561 cgggcttaat tgcaaggagc ttgcgcttct gcggcggctc cccgggcgag aggcgaattg
     1621 aaaccgccgc ccggtcaggt cgaacccaca gctcgtcgcg ccggatgcgc cgcggctcct
     1681 ctcccttggg cgcgataatg aactcggccg cgtcctcctg ggcattgatc gccgccacgt
     1741 atcgcgggcg attggtggcc gggtcgagga ccatcacccg gtcggtgaag ttgatgttgc
     1801 tgagcaccca ccagacgccg aaggcggcga tgccccagcg gacggtgaac ttcagccatt
     1861 tcttggtgcg gtcggtcatc cggttcggct ctcgcgtccc ccgccgtgag ggacgggtac
     1921 tttatcgacc gatccacagc gcgcatagga ggcgcggccg tatgatccgc ggatgcagat
     1981 cggcccggtg agagcaacgg cagcggcggt gatccttttc gttggcggtt gcgccgccat
     2041 tccgattgcg acggtgggca ccgtcgcggg gctggcggcg agcaccgtcg ataccggagc
     2101 cgatctctac aagatgggaa agctggatgc ggccgacctg gcgacctttg acgacgtcgt
     2161 cgcc
//
LOCUS       DATIMH010000503         6204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4160436, whole genome shotgun sequence.
ACCESSION   DATIMH010000503 DATIMH010000000
VERSION     DATIMH010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4160436"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VLI90_08830"
     CDS             <1..465
                     /locus_tag="VLI90_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14350.1"
                     /translation="ATIRGLIHGGQLITTAAGGALGYAIFGGTQVEVRFTLAGDATLD
                     RSVGVGDLGALATNYGTTAGAIWGQGDFDYNGTVDVADLGKLATNYSKSLSGGLGQSA
                     IPAAAAGAPASAVLLSYADDIAVDTSAAPASLDIAAARNRLFNDSTAIDELV"
     gene            626..2998
                     /locus_tag="VLI90_08835"
     CDS             626..2998
                     /locus_tag="VLI90_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14351.1"
                     /translation="MGQRAKSQRRRCVWSGLAAAAAAAVALVSPGNAALGTTVRVVSY
                     NVDCSDLTNNTDPKANANLPDVATVLQAIGVHQIGGNAQRFDVLGAQELLDTNNNTIT
                     SASLPALVNDLNAVYGAGAYAFDSTPDPTAGGVQFDGPSGLIYNTQTIQVVAAKALGY
                     SGTGAGSIPRAPMRYLLHPIGYSTSANFYMYVSHYKSTAASGSVRLQEATEIRADADA
                     LGPNVPILYTGDHNIFQDSAEPSYQKLLSTGNGQAHDAMNPTQDFVADAAHVNILTVG
                     PTAVQFRDDLQLTSGTVWNQSGTYTQNSLRLANGGNGAAIVFGNGSSPTVPAGGTGQP
                     FQGSINYAGNHTLDDLPNTSAVLTALTKASDHLPVVADYTLAGLTALPQTRYYVGPAS
                     GTWNTSSNWSTSDSGPGGASVPATGDTVIVDPSAGAAVSFDANYISPGVSELRLNGTG
                     GSTATVNQTANALVVSGNVLLGQAAGQKGSYSLSGGALTAGAIFVGSSSGGAGTFTLS
                     GSGTLNVTGSLTNYGTVTLGATQNWSAGASFNNAAGTATFNTDAGSAAAATLTVNVTG
                     GSVTFAAGQHLAGLNLSASGQALLTPAASAASPRVLNVASLSIIGSAKLDLSNDEMTA
                     TATADSIRTKLIGGAIFSSSSDATHTLGYNDIGGGQTEVRYTLLGDTDLNGSVDVGDL
                     GALASNYGISTDRTWSQGDFNYDDKADVADLGALATNYGVSLGAGSIDGASVAGPIAA
                     AAAVPEPGGMWALLSSAAILCQVRLRNRDMKRCSCPLSRSTGRGGKRAQK"
     gene            complement(3022..4422)
                     /locus_tag="VLI90_08840"
     CDS             complement(3022..4422)
                     /locus_tag="VLI90_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14352.1"
                     /translation="MQSTAAPAKRSTALAFVLACAPVLLVALMYIPDLGDDTLDYLHM
                     MNHEWDKVVQPFSARFLHPWLVSTATHASGTSLQTTAIVIDLAAFVGSVLALIILFRR
                     LRLPVMWLPPLMFSYAVVHTVSIIYMPDQFYTALLSVFLLALAFEQLAIAALLIIVLF
                     CARESTLVLGLVAAGVFAWRARRRSAPLDRSAARREYTWAVLFFAATIAGFLVNRYGS
                     NHASNVHHINPLLYLAVKAPYNFVKNILGVQIWTNTFVATQGDHPIYRWPLPRRLHLG
                     GIDTIGISDMSPVYPLNWLISIISTFGVMLTVVLQSIARRAQAFRNQNPLRRVSSAFG
                     ALLRGEPFVVQFAGAYGIINFLLVPLLGSAVHRYIYYAWPGFWIFAPVLLARRYDIFT
                     AAARPALWTLLVIHLALAWSRSVQLLWPDSSLFTLAVLAAVVVLQVFAWRLLARLEVG
                     AANTGAAPTAATNAAV"
     gene            complement(4700..5731)
                     /gene="xerC"
                     /locus_tag="VLI90_08845"
     CDS             complement(4700..5731)
                     /gene="xerC"
                     /locus_tag="VLI90_08845"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HSV14353.1"
                     /translation="MEKIFPPLVKQFLEYLKLEKHFSDYTVKSYGADLIQFGQFLAGA
                     IGQSHATPPSPELASLDIDSKLTRCEPLTVREFLAYLYAQNYTKSTTARKLATLRSFY
                     KFLIRRGLVSLNPLSTIRTPKQEKRLPKCLDLEQVQKLLEAPGDADLLSARDKAMLEV
                     LYSSGIRVSELVELEMADIDLQEGVLRVRGKGRKDRLTPIGSQAIRALQRYFELRALD
                     IKSQNNQTGRVFLNKHGQSLSTRSVRRKLDKYLVAAGLDPGISPHTLRHSFATHLLNN
                     GADLRSVQELLGHQSLSTTQVYTHLTTSRMKQVYDQAHPRADSETVEGNHTPIPHPAA
                     PSNPFAKAG"
BASE COUNT         1153 a         1893 c         2095 g         1063 t
ORIGIN      
        1 gcgacgattc gcggcctgat tcacggcggg cagttgatca ccaccgccgc cggcggcgcg
       61 ctcggctacg ccatcttcgg cgggacccag gttgaagtgc gcttcacact ggccggcgat
      121 gcgacgctcg atcgatccgt cggcgtgggt gacctgggcg cactggccac caactacggc
      181 acgaccgccg gcgcgatctg gggccaaggc gacttcgact acaacggcac agtggacgtt
      241 gcggacctcg gaaagctggc gacgaactac agcaagtccc tcagcggcgg attgggacaa
      301 tccgccatcc cggcggcggc cgccggcgcg cctgcgtccg cggttctttt gagctatgcg
      361 gacgacatcg ccgttgacac aagtgcagcg ccggcttcac tcgatatagc tgcggcccgg
      421 aaccggttgt tcaacgattc gaccgcgatc gacgagttgg tgtaaacgtc gccggtgtgc
      481 gcgagagggg cttgggaatc atcgcatggg ccgcatcgcc gcccggtttc cactcccatc
      541 cggcagaaaa atgttgcatc tacccccgtg gatgctacct tcaaccgcgg gcgatctgat
      601 cttccgcttc cgaatttaag gatctatggg gcagcgcgca aaaagccaaa ggcggcggtg
      661 tgtctggtcc gggctggcgg ccgcggcggc ggcggcggtg gcgttggttt cgccgggtaa
      721 cgcggcgctg ggcacgaccg tgcgcgtcgt ttcctataac gtcgattgtt ctgatctcac
      781 caacaacacc gatcccaagg ccaacgcgaa cctgccggac gtggcgaccg tgctacaagc
      841 gatcggcgtt catcaaatcg gcgggaacgc gcagcggttc gacgtgctgg gcgcacagga
      901 gctgctcgac acgaacaaca acacgatcac gtccgcgtcg ctgccggcat tggtgaacga
      961 cctgaacgct gtgtatggcg cgggggcgta tgcgttcgat tccacgcccg atcccaccgc
     1021 gggcggcgtg cagttcgatg ggcccagtgg attgatctac aacacgcaga cgatccaggt
     1081 cgtcgcggcc aaggcgctgg gctacagcgg caccggagcc ggcagcattc cgcgcgcgcc
     1141 gatgcgctat ctgctgcatc cgatcggtta cagcacgagc gcgaactttt acatgtacgt
     1201 cagccattac aaatcgaccg cggcttcggg ctcggtgcgg ctgcaggagg cgacggagat
     1261 tcgcgctgac gccgacgcgc tggggccgaa cgtgccgatc ttgtacaccg gcgatcacaa
     1321 catctttcag gacagcgccg agccgtcgta tcagaagctg ctttcaacag ggaacgggca
     1381 agcccacgat gcgatgaacc cgacgcagga tttcgtcgcg gacgcggctc atgtgaacat
     1441 cctgaccgtt gggccgacgg ccgtgcagtt tcgcgatgat cttcaattga ccagcggcac
     1501 ggtctggaat cagtccggca cgtacaccca aaatagcctg cgcttggcca acggtggcaa
     1561 cggcgcggcg atcgtcttcg gcaacggcag ttcacccacg gtgccggccg gcggcacggg
     1621 gcagccgttt cagggcagca tcaattacgc gggcaatcac acgctggacg atctgcccaa
     1681 caccagcgcg gtgctgaccg cgctgaccaa ggccagcgat cacctgccgg tggtggccga
     1741 ctatacgctg gcgggcctga ccgccctgcc gcagacgcgg tactacgtcg ggccggcgag
     1801 cggcacgtgg aacacgtcca gcaactggag cacgagcgac agtggacccg gcggcgcctc
     1861 ggtgcccgca accggtgaca ccgtcatcgt cgatccatcg gcgggcgcgg cggtttcgtt
     1921 cgatgccaac tacatcagcc cgggcgtcag cgaacttcgc ctgaacggca ccggcggctc
     1981 gaccgccacc gtcaatcaaa ccgccaacgc gctggtggtt tcggggaatg tcctgctcgg
     2041 tcaggccgcg gggcagaaag gctcgtatag cctttccggc ggcgcgctga cggcgggggc
     2101 gatcttcgtc ggcagcagct cgggcggcgc gggcacgttt acgctttccg gcagcggcac
     2161 cttgaacgtc accggcagct tgaccaacta tggaacggtg acgctcggcg caacgcaaaa
     2221 ctggtccgcc ggcgcgagct tcaacaacgc cgccggcacg gcaacgttca ataccgacgc
     2281 cggctccgcg gcggcggcga cgttgacggt gaacgtcacc ggcggcagcg tcacgttcgc
     2341 cgccggtcaa catctggccg ggttgaacct ttccgccagc ggccaggcgc tactgacccc
     2401 cgcggccagc gcggcgtcgc cgcgggtgct gaacgtcgca tcgctcagca tcatcggcag
     2461 cgccaagctt gacctgagca acgacgagat gaccgccacc gcgaccgccg attcgatccg
     2521 aacgaagctg atcggcggcg cgatcttttc gtcatcgagc gatgcaacgc atacgctggg
     2581 ctacaacgac atcggcggag ggcagacgga ggtgcgctac acgctactcg gcgacacgga
     2641 cctgaacggc agcgtggacg tcggcgatct gggcgcgctg gcgagcaact acggcatctc
     2701 caccgatcga acctggtcgc aaggcgattt caattacgac gacaaggcgg acgtcgccga
     2761 tctgggcgcg ctggcgacga actacggcgt gagcctgggg gccgggtcca tcgacggcgc
     2821 gagcgttgcc ggtccgattg ctgcagcagc cgcggtgccc gagccgggtg ggatgtgggc
     2881 gcttttgtcg agcgctgcga tactgtgcca ggtgcggctg aggaatcgtg acatgaagcg
     2941 atgctcttgt cccctctccc ggagtaccgg gcgaggggga aagcgggcac aaaagtaatc
     3001 ctaatgcgta atagcacatg cttagactgc cgcgttcgtc gccgccgttg gcgcggcgcc
     3061 tgtgttggca gcgccgacct ccagccgcgc gagcaagcgc caagcgaaaa cctgcagcac
     3121 cacgaccgcg gccaggacgg ctagcgtgaa caggctgctg tcgggccaca gcagttgtac
     3181 ggatcgcgac cacgccagcg cgagatggat gaccagaagc gtccacagcg ccggtcgcgc
     3241 ggcggcggtg aagatgtcgt atcgccgggc cagcagcacg ggagcgaaaa tccagaagcc
     3301 gggccaggcg taatagatgt atcggtggac ggccgagccg agcaacggga cgagcaggaa
     3361 gttgatgatg ccgtaggcgc cggcgaattg gacgacgaac ggttcgccgc gcagcagcgc
     3421 gccgaatgcc gacgatacgc ggcgcagcgg gttttgattc cggaacgctt gagcgcgccg
     3481 ggcgatcgac tgcagcacga cggtcagcat cacgccgaac gtgctgatga tgctgatcag
     3541 ccagttgagc gggtagacgg gcgacatgtc gctgatgccg atcgtgtcga tgccgcccag
     3601 atgcaatcgc cgcggcagcg gccagcggta gatgggatga tcgccctggg tggcgacgaa
     3661 cgtgttggtc cagatctgca cgccgaggat gttcttcacg aagttatacg gcgccttgac
     3721 cgcgagatag agcagcgggt tgatgtggtg cacgttggat gcgtggttgc tgccgtaacg
     3781 gttcacgaga aacccggcga tggtggccgc gaagaacagg acggcccacg tgtattcccg
     3841 gcgcgcggcc gatcgatcga gcggcgcgct gcgccgccgc gcgcgccacg caaacacgcc
     3901 ggctgccacg agcccgagca ccagggtcga ttcgcgggcg cagaacagca cgatgatcaa
     3961 cagggccgcg atcgccagct gctcaaacgc caatgcgagc aggaagacgg acaacaatgc
     4021 cgtgtagaac tggtccggca tgtagatgat gctaacggta tgaaccaccg cgtaggaaaa
     4081 catgagcggc ggcaaccaca tgaccggcag tcgcagccgg cgaaacaaaa tgatgagtgc
     4141 caacacggag ccgacgaacg cggcgaggtc gatgacgatc gcggtggttt gcagcgatgt
     4201 gcctgacgca tgagtggccg tgctgaccag ccaaggatgc aggaaccggg cgctgaaggg
     4261 ctgaaccacc ttgtcccact cgtggttcat catgtgcagg tagtccagcg tgtcgtcgcc
     4321 caagtcaggg atgtacatca gcgcgaccag gagcaccggc gcgcaggcga gaacgaaggc
     4381 gagagcggtg gagcgcttgg caggtgctgc ggtggattgc ataagggggt gtccgcctat
     4441 tgatcccgag ttgatgccgg cgctcgggtg ccggtgttcc agacgaacca attgtgatgg
     4501 cacggtatac cacacccgcg tttggccgac ttgcggattg gccgcgcttt tttaagtggc
     4561 gcgccgcgag gccgcggcgg ctaaacgacg cgctggtttg actcacgtac cgtccgctgc
     4621 tgcttcaaac gaaacagggc ctcgccaccg agagatggcg aggcccttct tctgcttcag
     4681 atggtcaccc gggcgatcat tacccggctt tggcgaacgg gttgctcggg gccgcgggat
     4741 gcgggatcgg ggtgtggttg ccttcgaccg tttcgctgtc ggctcgcggg tgggcctggt
     4801 cgtagacctg cttcatccgg ctcgtcgtca ggtgcgtgta cacctgcgtc gtgctcaggc
     4861 tctggtggcc cagcagttcc tgcacgctgc gcaggtcagc gccgttgttc agcaggtgcg
     4921 tcgcgaaact gtgccgcagc gtgtgcgggc tgatgccagg atcgagtccg gcggcgacga
     4981 gatatttgtc gagcttgcgg cggacgcttc gcgtgctcag cgactggccg tgcttgttca
     5041 ggaacacccg gccggtctgg ttgttctggc tcttgatgtc cagcgcgcgc aattcgaagt
     5101 aacgctgcag cgcgcggatg gcctggctgc cgatcggcgt gaggcggtcc ttgcggccct
     5161 tgccgcggac gcgcagcacg ccttcctgca ggtcgatgtc ggccatctcc agctcgacca
     5221 gctcgctgac gcggatgccg ctggagtaga ggacttcgag catcgccttg tcacgagcgc
     5281 tcagcagatc ggcgtcgccc ggcgcctcga gcagcttctg cacctgttcc aggtccaggc
     5341 acttgggcag gcgcttctcc tgcttgggcg tgcggatggt ggacagcgga ttgaggctga
     5401 cgagcccgcg gcggatgagg aacttgtaga agctccgcag cgtcgccagc tttcgggccg
     5461 tcgtgctctt ggtgtagttc tgcgcgtaga gatacgcgag gaactcccgg acggtcagcg
     5521 gctcgcagcg cgtcagcttg gagtcgatgt cgagcgacgc caactccggc gacggcgggg
     5581 tggcgtgcga ctgaccgatc gcgccggcca ggaactggcc gaactggatc aggtccgcgc
     5641 cgtagctctt gacggtgtag tcgctgaagt gcttctcgag cttgaggtac tcgaggaact
     5701 gcttgaccag cggcgggaaa atcttttcca cagtattcgc cgagaaggac agggaggcgg
     5761 ttgcgctgct cataggtaac cgtcgcaggc tacgcgcggg ttcgcagggg cgcaacgtga
     5821 aaacgtgcgc gctcgcgtgc ggggcgcggg gattcctgga acatgccagg cgattgttcg
     5881 gcgaaacgct cgcgtggcca cagtcgtgga cgatcctcgt cacacggcgt cgaggctctt
     5941 ttagctaagg aagcaaaaga atcgacggga gatgggcgcc ttgaattgtc agttgtctgt
     6001 tgtcagttgt ccgttgttca aaaacaacgc caccactgac cacggaccaa tgacatcatc
     6061 gcgatgccaa taacccttat gcagctttct tcggcaaata tgccattaat tctttgctta
     6121 aatgctgacc gagctggtgg gagaggacgg cggcgtcgaa ccagtcgaac gccatgtcgc
     6181 ggcgattgac catttcgatc aacg
//
LOCUS       DATIMH010000504         1956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3295013, whole genome shotgun sequence.
ACCESSION   DATIMH010000504 DATIMH010000000
VERSION     DATIMH010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3295013"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1832)
                     /locus_tag="VLI90_08850"
     CDS             complement(<1..1832)
                     /locus_tag="VLI90_08850"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSV14354.1"
                     /translation="MTSVESLRAPWDVTPGGTAFPRAPGPATSLIMDLLATLPALLVL
                     ARRSIDKDFRLTPRWSHLPMLALGAWVMLSTTWASDTFAALVHASNWLAGLALMWAFS
                     QLVRSWVRLRIVASVGFGLLLVLVAQSVLYRVIEVPETIRYWNTNRDALLKERNWEPG
                     SFQARQFEHKITSGELPGFFASPNTFAAVGVMLLFVSAGIGVQKTRDGDGAAWLALSA
                     VAIAAAGWVIVEARSKTAGATPVLGVSMLLAIALWHDRLRRHATPFYWLGVAAFFLAA
                     AAIVGHGLYHHGLFPGHFSNSLDFRWKYWMAAAQIFKQHPIRGVGWDSFGQYYLAARL
                     PEASEEVKDPHNFLVRVFVETGMVGGVLVIAWMLRLWWELTRPSNDTNQPSTESCGSA
                     TVGAILWIAVLGITISVIANVDFTQSIADVALELMRRVLYLMVLAAGSIAGTMLSPHR
                     RELDGRAAPWLLYFVLVALGLFLIHNLIDFSMFEVGPLFLFLMLVGAALGVAPATARA
                     GRSRAWAIALLIVAMIGWLIAAARLVGPVAVAEQSAFDADEAIRTAPADQPAQLEMRY
                     RQAATDLSDALARVPWNTDYALRAAKAWLGAGQVDRAEDMLAAA"
BASE COUNT          382 a          679 c          610 g          285 t
ORIGIN      
        1 atcgccgccg ccagcatgtc ctcggcccgg tcgacctgcc ccgccccgag ccacgccttc
       61 gccgcgcgca gcgcatagtc cgtgttccac ggcacccgcg ccagcgcatc agacaaatcc
      121 gtcgccgcct gccgataacg catctccaac tgcgccggct gatccgccgg cgcggtgcgg
      181 atcgcctcgt ccgcgtcgaa tgcagactgc tccgccacgg ccaccggccc aaccaatcgc
      241 gcggccgcga tgagccaacc gatcattgcg acaatcaata gcgcgatcgc ccaagcgcgc
      301 gatcgacccg ctcgcgccgt cgccggcgcc acgcccagcg ctgcgccaac taacatgagg
      361 aacaaaaaca gcggcccgac ttcgaacatc gagaagtcga tcaggttgtg gatcagaaaa
      421 agtcccagcg cgacgagcac gaagtacaac agccacggtg ccgcccggcc gtccagctcc
      481 cggcggtggg gcgacagcat cgtccccgcg atgctgcctg cggccagcac catcaggtac
      541 agcacccgcc gcatcagctc tagcgccacg tcggcgatcg actgggtgaa atcgacgttg
      601 gcgatgacgc tgatcgtgat gccgagcacc gcgatccaca gaatcgcgcc gacggtggct
      661 gagccgcatg attcggttga tggttggttt gtgtcgtttg atggccgcgt cagttcccac
      721 caaagccgaa gcatccacgc gatgacgagc acgccgccga ccatgccggt ttcgacgaag
      781 acgcgaacga ggaagttgtg cgggtccttc acctcttcag acgcttcggg caatcgcgcg
      841 gccaggtaat actgcccgaa actgtcccac ccaacgccgc ggatcggatg ctgtttgaag
      901 atctgcgccg ccgccatcca gtacttccag cggaagtcga ggctgttgct gaaatgacct
      961 gggaacaggc cgtgatggta aagcccatgc ccgacgatcg ccgcggcggc gaggaagaac
     1021 gcggcgacgc cgagccagta aaatggcgtc gcgtgtcgcc gcagtcgatc gtgccagagc
     1081 gcgatcgcca gcagcatcga aacgcccagc accggcgtcg cgccggcggt tttgcttcgc
     1141 gcttcgacga tcacccaccc cgcggcggcg atcgcgacgg cgctgagcgc cagccaggcg
     1201 gccccgtcgc cgtcccgcgt cttctgcacg ccgatgcccg ccgacacaaa cagcagcatc
     1261 accccaacgg cggcgaacgt gttcggcgat gcgaagaacc cgggcaattc gccgctggtg
     1321 atcttgtgct cgaattgccg ggcctggaac gagccgggct cccaattgcg ttccttcagc
     1381 agcgcgtcgc ggttggtgtt ccaatatcga atcgtctcgg gcacttcgat gacgcgataa
     1441 agcactgatt gtgcgacgag caccagcagc aggccgaatc cgacggacgc cacgatgcgc
     1501 agccgcaccc agctgcgcac cagctgcgag aacgcccaca tcaaggccaa ccccgcgagc
     1561 cagttcgatg catgaacgag cgcggcgaac gtatcgctcg cccaggtggt gctgagcatc
     1621 acccacgcgc ccagcgccaa catcggcaga tgactccacc gcggcgtgag gcggaagtcc
     1681 ttgtcgattg accgccgcgc gagcacgagc agcgccggca gcgtggcgag caggtccatg
     1741 atgagcgacg tcgccggccc gggcgcgcgc ggaaacgccg tgccgcccgg tgtgacatcc
     1801 cacggggcgc ggaggctttc gacgctggtc aaccgtgtga tgacgagggc gattgcgaag
     1861 acgaacgcga cctgcgtcaa ccatcgccac ggcgaaggtg aggtgctcga gtcgatcgac
     1921 atcgacatcg gcgcatggcg atttcgagcg gtggag
//
LOCUS       DATIMH010000505         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5606943, whole genome shotgun sequence.
ACCESSION   DATIMH010000505 DATIMH010000000
VERSION     DATIMH010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5606943"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1385
                     /locus_tag="VLI90_08855"
     CDS             123..1385
                     /locus_tag="VLI90_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HSV14355.1"
                     /translation="MHVVIFEGSHWRTFAPFALSRPVFLLPCGAGTLLDKQIRATRPS
                     RVTLWVRPELEDYCRRNVLPTLPCPGDVNKPLGDEVTLLMSGRTLHLSQYNIEDGESI
                     VLDERPDGGDRVMRSALVRSPGLTPDDVLQRSDRWLRLLDLPASTPQSRLPEYVWDLI
                     KWNEEAIVADSIAMTEPSQPLTPGPYHAVEESNIWLGNKVHLGPSCVLDASKGPIVLA
                     ENVNIGANAVIQGPVYLGPHTQITPLALIRAGTSTGPMCKLGGEVSNSVILGFTNKPH
                     EGFLGDSYLGEWVNLGAGSTTSNLKNTYGEITMPVEGRQVKTGRRFMGSLIGDHSKCA
                     IGTRLMTGSYVGYACMVGMSAYPPRFIPSFSWLTDRGAEAYRMEKVAEMIKSVFIRRE
                     RVFNETDQQMLDYVAGAAKLVEKAPAGK"
     gene            complement(1536..2090)
                     /locus_tag="VLI90_08860"
     CDS             complement(1536..2090)
                     /locus_tag="VLI90_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14356.1"
                     /translation="MRIIPSYVLPTILLLLVGCAHYEFALVRPSDLARHIGDNAEGRF
                     TLDPLEYRMQSVEGRLVILPFNPTSDTIQLLGHLSSVVDPGGQSHPLQDQTIAPRSFV
                     KLILPPLRPQVAPSGPTIGIGFGVVGDRSSSRVDEPRYLDVYYANDPIYWEWEGESDV
                     RLTLVYHRGERQFTHEFTFHRKRM"
     gene            2420..>3589
                     /locus_tag="VLI90_08865"
     CDS             2420..>3589
                     /locus_tag="VLI90_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14357.1"
                     /translation="MTWNPPTESWRRRLLRRVVDRNPFYLISAACMLGGCLALTNSLS
                     WLSLPLPRLLTLIATINVYEGMLIVLAVILIGRGLLRDGKILLILEAFFLVDVTFLDA
                     EVVTANLNVGMLVSGVLLALAAVKLWCVMRAIGADLSPARFAYALSQVAALLAIPCVL
                     RAIDHGALSPRALYATWWVVGLMPVVQEIATGIWGVGHRQRVPAVAAVYLVLPWISLA
                     AHVGILHYVYGVAYYGAMAAPMLLGLTLLLNRATPESLHARANLFALRAIMPIAAIVV
                     SMNHPLALGMTLKPFAATHVTTTMLAVSGAYLVYVYCFLWHAAGVFLVGGAGVALMYL
                     FGPSVGQIENTAWRGWSWSTDVADRLVSKTLAQWGVVSVLASLVFLIIGAGISLSA"
BASE COUNT          590 a         1130 c         1185 g          684 t
ORIGIN      
        1 cggcatcacc gcatggtcag gcgttacgaa ttacgtctcc gatcacgtat tcacgtttca
       61 cgtattcaga atcacgccca cgtgattcaa gtccgccggg tccgacggta tcataccgcc
      121 ccatgcacgt cgtcatcttt gagggctcgc attggcggac attcgccccc tttgcattga
      181 gccggccggt ctttttgctt ccctgtggcg cgggcacgct gctcgataag cagatccgcg
      241 cgacgcgccc gtcgcgcgtg acgctttggg tgcgccccga gctggaggac tattgccggc
      301 ggaacgtcct gcccacgctg ccgtgcccgg gcgatgtgaa caagccgctc ggcgatgagg
      361 tcacgctgct gatgagcggg cgaacgctgc acctgtcgca atacaacatc gaagacggcg
      421 agtcgatcgt cctggacgaa cgccccgacg gcggggaccg cgtgatgcgg tcggcgctgg
      481 tgcgcagccc cggcctgacg cccgacgatg tcttgcagcg cagcgaccgt tggttgcgtt
      541 tactagattt accggcatcg acgccgcaat cgcgcttgcc ggagtacgtt tgggatttaa
      601 tcaagtggaa cgaggaggcg atcgtcgcgg attcgatcgc gatgaccgag ccgtcgcaac
      661 cgctgacgcc ggggccatat cacgcggtgg aagagagcaa catctggctg ggcaacaagg
      721 tgcaccttgg acccagctgt gtgctggatg cgtccaaggg cccgatcgtc ttggcggaga
      781 acgtgaacat cggcgccaac gcggtcatcc agggtccggt ttatctgggc ccgcacacgc
      841 agatcacgcc gctggcgctg atccgcgccg gcaccagcac cgggccgatg tgcaagctgg
      901 gcggcgaagt ctcgaacagc gtcatcctcg gtttcaccaa caagccgcac gaagggttcc
      961 tcggcgacag ttacctcggc gaatgggtca acctcggcgc cggcagcacg accagcaacc
     1021 tgaaaaacac ctacggcgaa atcacgatgc ccgtcgaagg ccggcaagtg aaaaccggcc
     1081 gccggtttat ggggtcgctc atcggcgatc attccaagtg cgcgatcggc acgcgcttga
     1141 tgaccggcag ctacgtcggt tacgcctgca tggtcggcat gtccgcctat ccgccgcggt
     1201 tcatccccag cttcagctgg ctcaccgacc gcggagccga agcgtatcga atggaaaagg
     1261 tggcggagat gatcaagtcc gtcttcatcc gccgcgaacg cgtctttaat gagacggacc
     1321 agcagatgct ggattacgtc gccggcgcgg cgaagttggt ggagaaagcg ccggctggga
     1381 agtgaccttg ctgtcgcaca ctttccccct cgcccggtaa tccgggagag ggtcgaggtg
     1441 agggtgattt cgatcgtcga acggctctcg gaaactacct caccctcacc ctaaccctct
     1501 cccggagtac cgggcgaggg gactagagcg acgcgctaca tccgcttccg gtgaaatgta
     1561 aactcgtgcg tgaactgccg ctcgccgcgg tgatacacga gcgtgagccg cacgtctgac
     1621 tcgccttccc attcccagta aatcggatcg ttcgcgtagt acacgtccag ataccgcggc
     1681 tcgtcaacgc gcgacgacga gcgatcgcca accacgccga acccaatgcc gatcgtcggc
     1741 cccgacgggg ccacctgcgg gcgcagcggc gggaggatca gctttacgaa cgacctcggc
     1801 gcgatcgtct ggtcctgcag cgggtggctc tgcccgccgg ggtccaccac ggagctgaga
     1861 tgcccgagca gctggatcgt gtcgctggtc ggattgaacg gcaggatgac cagccggccc
     1921 tcgacgcttt gcatgcggta ttcgagcggg tcgagcgtga agcgcccttc ggcgttgtcg
     1981 ccgatgtgtc gcgcgagatc ggagggtcgt acgagagcga attcgtaatg agcgcagcca
     2041 acgaggagga gcaggatcgt cggcagcacg taggatggga tgattcgcac gaggagattg
     2101 taaagcgagc gttggtgacg gtgccatcga caaagcgcag cgcgaatcgt tccgcgccat
     2161 ctttgatagc cgcaggcgat agccagcggg agctttcgca taccggcccg gttcgatgtc
     2221 tcgcgccccc gctggctgtc gcctgcggct atcaatgagt cgcttgcgga atcggcgcgg
     2281 ggcgtctctg cgactttcgc acgcctcacg cgtgcatctc ttgtgacccg cggattgcca
     2341 ttcgcgttcg gtcgttcggg gttggatcgc ctccgcccgc ccaccgcttc aagtccaccg
     2401 ctttccagag gagcttccta tgacgtggaa cccgccaacg gaatcgtggc gacgccggct
     2461 gctgcgcagg gttgtcgatc gcaatccgtt ctacctcatc agcgcggcct gcatgctcgg
     2521 cggctgcctg gcgctgacca actcgctctc ctggctgtcg ctgccgctgc cgcggctgct
     2581 cacgctgatc gccaccatca acgtgtatga aggcatgctc atcgtgctgg cggtgatcct
     2641 gatcggccgc gggctgctgc gcgacgggaa gattctgctg atcctcgagg cgtttttcct
     2701 ggtcgatgtg acgttcctgg acgccgaggt cgtcacggca aacctgaacg tcggcatgct
     2761 ggtcagcggc gtgctgctgg cgctggcggc ggtgaagctg tggtgcgtga tgcgcgcgat
     2821 cggcgcggat ttatcgcccg cgcgtttcgc ttatgcacta tcgcaagtgg cggcgctgct
     2881 ggccatccca tgcgtcttgc gcgcgatcga tcacggcgcg ctctcgccgc gcgcgctgta
     2941 cgcgacgtgg tgggtcgtcg gcctgatgcc ggtcgtgcag gagatcgcca ccggcatctg
     3001 gggcgtcggc catcgccagc gcgtccccgc ggtggcggcg gtgtatctgg tgctgccgtg
     3061 gatctcgctc gcggcccacg tcggcatcct gcattacgtc tatggcgtcg cgtattacgg
     3121 cgcgatggcc gccccgatgc tgctgggatt gacgctgctg ctgaaccggg cgacgcccga
     3181 gtcgctgcac gcgcgggcga acctgttcgc gctgcgcgcg atcatgccga tcgcggccat
     3241 cgtcgtgtcg atgaatcacc cgctggcgct ggggatgacg ctcaagccgt tcgcggcgac
     3301 acacgtgacg acgacgatgc tggccgtcag cggagcgtac ctggtctacg tctactgctt
     3361 cctatggcac gccgccgggg tgttcctcgt gggaggagcc ggcgtcgcgc tgatgtacct
     3421 gttcgggccg agcgtgggcc agatcgagaa caccgcgtgg cgcgggtggt cgtggtctac
     3481 tgacgtcgcg gaccgcctgg tgtccaaaac gctggctcaa tggggcgtgg tgtcggtgct
     3541 ggcgtcgttg gtgttcctga tcatcggcgc ggggatcagt ctgtcggcc
//
LOCUS       DATIMH010000506         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_699932, whole genome shotgun sequence.
ACCESSION   DATIMH010000506 DATIMH010000000
VERSION     DATIMH010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699932"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VLI90_08870"
     CDS             <1..1138
                     /locus_tag="VLI90_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSV14358.1"
                     /translation="SFRDWVLAQDRFDARFRLPSLPRSKRTMIHKYLATAVSILLLAP
                     LTACEKRDTTATTSTSAAGGKIRVVYIPKNTGNPYFNGVQEGFTRGCKEQNIDCLSTG
                     PTTADATSQIPILKDQIQQAVNVICICPNSPDALNAVLDDARAKGIKVITVDADLTGN
                     ESHRDACVLPTDFNALGASQIELLGSLINYEGKFAILSATTDAPNQNYWIKGMKEALK
                     DPKYAKMELVDTVYGDDKPEKSRKEAEGLLTKYPDLRAILAPTSVGVEQAAKTIEASG
                     TYPGGPNAKGPGVVVTGLGTPDQMRSYIEKGIIQKVALWSPPDEGYVTAYLAGNIAQN
                     KLKLDRGGSFEVPSLGTRQLGNNNVVITGPPLVFTKENIGNYHF"
     gene            complement(1288..1539)
                     /locus_tag="VLI90_08875"
     CDS             complement(1288..1539)
                     /locus_tag="VLI90_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14359.1"
                     /translation="MKRRAVRRVWSLSKYHRMANHTIDDLAALHAEAQRHQSDIADDQ
                     HLLRSCFEGAGLALTIGHAQVAAAASRRCSTSAPRGRGG"
     gene            complement(1641..3128)
                     /locus_tag="VLI90_08880"
     CDS             complement(1641..3128)
                     /locus_tag="VLI90_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HSV14360.1"
                     /translation="MRSIATAAALAVAAVMTGGCETKSFLDPGEMGRYEKGPALQKPI
                     LNSLSSFDRSIDDPNDEFVNATDVRPEDLQVVSHDYVIGKNDVVNVSVTDLVGPGVET
                     QKITRVSESGRISLPLIGQVQAEGLTEAQLEQAIVDAYRNANLIQNAQVSVTVTEPRG
                     RTFSVLGAVNNPGQYGIYQSDFRLLDALVTAHDVMQSVDTLYVIRQLDEEPGNVKGPA
                     TTPPPPTGGGAGTGTGTGTDTLAPKSPDILAPISRAHFGGTNVALLQTTGNAGTAGDH
                     QLVLPNGQAPASTETTPPPPVDTGAGALAPTPGAGGAGGAGGASTPAPAPATSFQFVQ
                     PKAPSETRVIRIPLNALKNGDLRYNIVIRPRDLVLAPTPVIGEYYMGGHVSRVGVYSL
                     TARKITLKEAIVSAGMLDGLSIPQRTDIIRRLGPDREVFARINLDAIFGGTQPDIYLK
                     PYDQIMVGTNLIAPFLAAIRGGFRLTYGFGFLYDRNYAPQQSIGG"
BASE COUNT          619 a         1014 c         1036 g          573 t
ORIGIN      
        1 ttcttttcgc gactgggtgt tggcgcaaga tcgttttgac gcccgcttcc gattaccatc
       61 gctgcccagg agcaaacgaa ccatgatcca caaatacctc gcaacggccg tgtccatcct
      121 gctgctcgcg ccgctcaccg catgtgagaa gcgcgacacc accgctacca cgtccaccag
      181 cgcagccggc gggaagatcc gcgtcgtata catccccaag aacaccggca acccatactt
      241 caacggcgtc caggaaggct tcacccgcgg gtgcaaagag cagaacatcg actgtctctc
      301 caccggcccg acgacggcgg acgcgaccag ccagatcccg atcctgaagg accagatcca
      361 gcaagccgtc aacgtcatct gcatttgccc caacagcccc gatgcgctca acgcggtgct
      421 ggatgacgcg cgggcgaagg gcatcaaggt catcacggtg gatgcggatt tgaccggcaa
      481 cgaatcgcac cgtgatgcgt gcgtgttacc gaccgatttc aatgcgctgg gcgcgagcca
      541 gatcgaattg ctcgggtcgc tgatcaatta cgaaggcaag ttcgccatcc tgtcggccac
      601 caccgatgcg ccgaatcaga attactggat caagggaatg aaggaagcgc tgaaggaccc
      661 gaagtacgcg aagatggagc tggtcgatac ggtgtatggc gacgacaaac ccgaaaagag
      721 ccgaaaggaa gccgagggat tgctgaccaa gtacccggac ctgcgcgcga tcctcgcgcc
      781 gacgtcggtc ggcgttgaac aagccgcaaa aacaatcgaa gcctccggca cctaccccgg
      841 tggcccgaac gccaaaggcc ccggcgtcgt cgtcaccggg ctaggcacgc cggaccagat
      901 gcgcagctac atcgaaaaag gcatcatcca aaaggtcgcg ctctggagcc cccctgacga
      961 aggctacgtg accgcgtacc tcgccgggaa cattgcccag aataaactga agttagaccg
     1021 cggcggcagc ttcgaagtcc ccagcctggg cacccggcag ctgggtaaca ataacgtcgt
     1081 gatcacgggt ccgccgttgg tgtttacgaa ggagaatatt gggaattacc acttctgatt
     1141 gaacgccgtc gaaatcgtca ggcgtggcgt cgtgcgatgt gttatcgcct gagaaccgtc
     1201 tgcagtatcg ccaaggcgcc aggaaggtcg aaactgaaat cgatggcgct ccggcaatcc
     1261 gcggtgatat aaaaacggga caatcgttta gccgccgcgg cctcgcggcg cggacgtcga
     1321 acagcgccgc gaagccgcgg cggctacttg agcgtgccct attgttaacg caagccccgc
     1381 cccctcgaag cacgagcgga gcagatgctg gtcgtcagcg atgtcactct ggtgtcgctg
     1441 ggcctcggcg tgcaacgccg ccagatcgtc gatcgtgtgg ttggccatgc ggtggtattt
     1501 gctcaggctc cagacccgac gaacggcgcg acgcttcata gcggtcggac gtattgcaga
     1561 aacgcacaag cctgcgttgt gaggggtgaa cctcacgccg caggcttgcg cttttgagtc
     1621 tctgcgatct tgcgcgacgg ttagccgccg atcgactgct gcggggcgta gtttcggtcg
     1681 tataggaaac cgaagccgta ggtcaggcgg aacccgccgc ggatggcggc caggaacggc
     1741 gcgatcaagt tcgtgccgac catgatctgg tcgtacggct tgaggtagat gtccggttgc
     1801 gtgccgccga agatcgcatc gaggttgatg cgggcgaaga cttcgcggtc gggaccaagc
     1861 cggcggatga tgtcggtgcg ctgcgggatg gacaagccgt ccaacatgcc ggccgacacg
     1921 atcgcttcct tcaacgtgat cttgcgcgct gtcaggctgt agaccccaac gcggctgacg
     1981 tggccgccca tgtagtattc accgatgacc ggcgtggggg cgagcacgag atcgcgcggg
     2041 cggatgacga tgttgtagcg caggtcgccg ttcttcagtg cgttgagcgg aatgcggatg
     2101 acgcgcgttt cggacggcgc cttgggctga acgaactgga aggacgttgc cggcgcaggg
     2161 gccggcgttg aagcgccacc cgcgccaccc gcgccacccg cgccgggcgt gggggcgagt
     2221 gcgccagcgc cagtatccac cggcggcggc ggggttgttt cggtggaggc cggcgcttgt
     2281 ccgttgggca acaccaactg gtggtcgccc gcggtgccgg cgttgccagt ggtctgcagc
     2341 agcgcgacgt tcgtcccgcc aaagtgcgcc cgcgagatcg gggcaagaat atccggcgac
     2401 ttcggggcca gcgtatcggt tcccgtgccc gtgcccgttc ccgccccacc accggtcggc
     2461 ggtggcggtg tggttgccgg ccccttcacg ttgccgggtt cttcatccag ctggcggatc
     2521 acgtacagcg tgtccacgct ctgcatcacg tcgtgcgccg tcaccaacgc atcgagcagg
     2581 cggaagtcgg attgataaat gccgtattgc ccagggttgt tcaccgcgcc cagcacgctg
     2641 aacgttcgcc cgcgcggctc ggtcaccgtg accgacacct gcgcgttctg gatcaggttc
     2701 gcgttgcggt acgcatcgac gatcgcctgc tccagctgcg cttcggtgag gccttcggct
     2761 tgcacctggc cgatcagcgg caggctgatt cgaccgcttt cgctgacgcg cgtgatcttc
     2821 tgcgtctcga cgccaggacc caccaggtcc gtcaccgaca cgttcaccac gtcgttcttt
     2881 ccgatcacgt agtcgtgcga gacgacctgc agatcttccg ggcgaacatc ggtggcgttc
     2941 acgaattcgt cgttcggatc gtcgatgctg cgatcgaagc tcgacaggct gttcaggatc
     3001 ggcttctgca acgccgggcc cttctcgtag cggcccattt cacccgggtc gaggaagctc
     3061 ttggtttcgc agccgccggt catcacggcg gcaacggcca acgccgcggc ggtcgcgatg
     3121 gaacgcacgg cgacggcgga cttgcggcca gacccggcag tgatcgtgcc acgctgaaga
     3181 agacggggga gatgcaagcg ctgctccttt caagtcgcgc gcccatttga ggaggcgcaa
     3241 gc
//
LOCUS       DATIMH010000507         3952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_700198, whole genome shotgun sequence.
ACCESSION   DATIMH010000507 DATIMH010000000
VERSION     DATIMH010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700198"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1757
                     /locus_tag="VLI90_08885"
     CDS             129..1757
                     /locus_tag="VLI90_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14361.1"
                     /translation="MESAIGGNFADRSLDAAAELPLWQRVTFCDWLLAWADRHRWWLF
                     AGVACFYLAAFNGQWRLQPDTALYLSLGRNLAEGKGYTYLGRPHHLAYPGWPWLIAVT
                     FKLFGTQSLLPIHVVLFLIAIATIALSYRLFLLHSGRPTAVIVAVGLAVTKTFFTFGF
                     ELWSDMLFAAGVMAFLAGYEAVWRERPPGVNEPAHRARWYDFGLLVGGLAVAVVTRPT
                     MWPLIVAITGAMLYSAIKGHVRWQRYVGAAALITVVAAATFALARHRTAAGGEQITGE
                     YERYALQQASHLSQTLARAVTVNLRDLMDWAASDTLFQVRFGIFNRALSLLVIALGIS
                     LFRERVLWGLWFCALLITIVLVLALDRYFLPVIPLLVFAWWQLLVRINRALPRAWGNL
                     VFLGLLGAAMGINISKCGGIVIQQRTRPFLAHYSRGEYAAVPALAAEIAKHTGDDAVV
                     LIKPPYARVTEFLSARYVVNGFDLLSGFAAGTGALSRGPVYVVEPVDADIRDLLEREK
                     LRLGPVVWDSAAGSSAPASPYALRLRSTQKADLR"
     gene            complement(1754..3361)
                     /locus_tag="VLI90_08890"
     CDS             complement(1754..3361)
                     /locus_tag="VLI90_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14362.1"
                     /translation="MSSPSTSRILWASLGVFLLALAVRWPHLSQGLWFDEMVTLVDYV
                     LQPWQHVVAAQPGDYVPNNHVLHTVLAKLTYSLGPHNENGGTGPNEPLLRVPALLASC
                     LLPIALAWPLRKSRPIIALVIALLAAMHPWLVAFGTEARGYSMMLLFGVIATNLLPDG
                     QRGRWPIGYALALAAAIYTLPIAVLLIVAHGVAAVLRLPLRSDPSTQQGRDRGFGIWL
                     RGAIMAVVLSALLYAPMARAMMQYFRNPFEPTISYREFLDHLPRYALAGERLPRHPGP
                     APTVDPPAASVYWALPVLAIVIGSIFAWPIEPLRPLLVTLGVTSVCGIVLAGVRPAAC
                     EVRFVAWAGLWLIVAVTAILVSPTQRWAKSVAVGAMALQLVWMGMRDVQMPPNEPVRE
                     AMARADALAPPDHAIVIGSLGAREAAYLYGYEIAHHDMTTGHNMPLLLAAEQRVIDAT
                     GHKPWIVMLYEDLARRRDRRDQQDIRGMWTHLMKNYYLKQRLPGRVAPVAIYAPRETR
                     ADVAAAPNQPLAASQTNHAAMAESVKR"
     gene            complement(3421..>3952)
                     /locus_tag="VLI90_08895"
     CDS             complement(3421..>3952)
                     /locus_tag="VLI90_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14363.1"
                     /translation="AGISYAIVGGMAVNAHHHERTTKDVDFLLSAAGFAAFRQLMSGE
                     FSTVPGRPRRFVDAANGVTFDILVTGQYPGSGQPGPISFPDPATVGQTVGDLRVVDLP
                     TLVQLKLAAGRYQDFADVVNLIRANALDESFQQRLAPSVHADFIECLEEMRREDEYEA
                     RQDQNFRDAQGESGDH"
BASE COUNT          661 a         1230 c         1337 g          724 t
ORIGIN      
        1 ccgcaagcga ctgccaagcc cctcgcgcaa gctgtagctc accggtgccc cggcaagatc
       61 cttcgactgc gcacgagtgc gtgcttcgct caggatgaca cgtggcgcct ttaactaggg
      121 aaacgttttt ggaatcagcc atcggcggca acttcgcgga tcgttcgctt gacgcggcgg
      181 cagagctgcc gctgtggcag cgcgtgacgt tctgcgattg gctgctcgcg tgggccgacc
      241 ggcatcggtg gtggctgttt gccggcgtcg cgtgtttcta cctggcggca ttcaacgggc
      301 agtggcgctt gcaaccggac actgcgcttt atctatcgct cggtcgaaac ctggccgagg
      361 gaaaaggcta cacctacctg ggccggccgc accatctggc gtatcccggt tggccgtggt
      421 tgatcgcggt gacgtttaag ctgttcggca ctcaatcgct gctgccgatc cacgtcgttc
      481 tgtttctgat tgcgattgct acgatcgctt tgagctaccg cctgttcctg ctgcacagcg
      541 gccggccgac ggccgtcatc gtcgcggtgg gattggcggt taccaaaacc ttcttcacgt
      601 tcggcttcga gctgtggagc gacatgctct tcgccgcggg ggtgatggcg tttctggccg
      661 ggtatgaagc ggtctggcgc gagcgcccgc cgggcgtcaa cgagccggcg catcgggcgc
      721 gatggtacga tttcggatta ctggttggcg ggttggcggt ggcggtggtg acccgaccga
      781 cgatgtggcc gctgatcgtc gccatcaccg gcgcgatgct ttactcggcg atcaagggtc
      841 acgtgcgctg gcagcgttat gtgggggcag ccgcgctgat caccgtcgtc gccgcggcga
      901 cgttcgcgct ggcgcggcat cgcacggcgg cggggggcga gcagattacc ggtgaatacg
      961 agcgctacgc gctgcagcag gcgtcgcact tgagccagac gcttgcgcga gccgtgactg
     1021 tgaacttgcg agacctgatg gactgggcgg cgtccgacac gctgttccag gttcgcttcg
     1081 ggatcttcaa tcgcgcgctc agcctgctgg tcatcgcgct gggcatcagc ctgttccgcg
     1141 agcgcgtgct gtggggacta tggttctgcg cgctgctgat cacgatcgtt ctggtgctcg
     1201 cgctcgatcg atatttcctg ccggtcatcc cgctgctggt gttcgcgtgg tggcagttgc
     1261 tcgtgcggat caaccgcgcg ctgccgcggg cgtggggcaa cctggtgttc ctcgggctgc
     1321 tcggggcggc gatggggatc aacatcagca agtgcggcgg catcgtcatc cagcagcgca
     1381 cccgcccatt tcttgcgcat tacagccgag gcgaatacgc cgctgtcccg gcactggcgg
     1441 cggagatcgc caagcacacg ggcgacgatg cggtcgtcct gatcaaaccg ccgtatgcgc
     1501 gcgttacgga gttcttgagc gcgcgatatg tcgtgaacgg ttttgatttg ctcagcgggt
     1561 tcgccgcggg cacgggggcg ctatcgcgtg ggccggtgta tgtcgtcgag ccggtggacg
     1621 cggatattcg tgatttgctg gaacgcgaga agctgcgatt ggggccggtc gtgtgggata
     1681 gcgccgcagg ttcatcggcc ccggcctcac catacgcgct gcgactgcgt tcgacgcaaa
     1741 aggctgatct tcgttagcgt ttaacagatt ccgccatggc agcgtgattg gtctgcgaag
     1801 ccgcaagcgg ctgatttggc gctgccgcca catccgcccg ggtttcgcgc ggggcataga
     1861 tcgccacggg cgccacgcgg ccgggtagtc gctgcttgag gtaatagttc ttcatgaggt
     1921 gcgtccacat cccgcggatg tcttgctggt cccgccgatc gcgccggcgg gcgaggtctt
     1981 catacagcat gacgatccac ggcttgtgcc ccgtcgcgtc gatcacccgc tgctcggctg
     2041 ccagcagcag cggcatgttg tgacccgtcg tcatgtcgtg atgcgcgatc tcgtacccgt
     2101 aaagatacgc cgcctcgcgc gcgccgagcg atccgattac gatcgcatga tccggcggcg
     2161 ccaaggcatc ggctcgcgcc atcgcctcgc gcacgggttc gttcggcggc atctgcacgt
     2221 cgcgcatgcc catccacacg agctgcaaag ccatcgcgcc gacggccacg cttttcgccc
     2281 atcgctgcgt gggcgagacg aggatggccg tcaccgcgac gatgagccag agaccggccc
     2341 aggcgacgaa gcggacctcg caggccgcag gacggacgcc ggccaggacg atgccgcaaa
     2401 cggacgtgac gccgagagtg accagcagcg gtcgcagcgg ctcgatgggc caggcgaaga
     2461 tcgatccgat gacgatcgcc agcaccggca gcgcccagta gaccgacgcc gccggaggat
     2521 ccaccgttgg ggcggggccg ggatgccgcg ggaggcgttc gcccgccagc gcatagcgcg
     2581 gcaggtgatc gaggaactcg cgataggaaa tggtcggttc gaacgggttg cggaagtact
     2641 gcatcatcgc gcgtgccatc ggcgcgtaga gcagggcgga gaggacgacg gccatgatcg
     2701 cgccgcgcag ccagatgccg aagccgcggt cgcgcccctg ctgcgtcgac gggtcggacc
     2761 gcagcggaag ccgcaacacg gcggcgacgc cgtgcgcgac gatcagcagc accgcgatcg
     2821 gcagcgtgta aattgccgcc gctagtgcca atgcatatcc gattggccag cgacctcgct
     2881 gtccgtccgg cagcaggttg gtcgcgatga cgccgaagag cagcatcatc gagtacccgc
     2941 gcgcctcagt gccgaaggcg accagccacg ggtgcatcgc cgcgagcagc gcgatcacca
     3001 gcgcgatgat gggtcggctt ttgcgcagcg gccaggccag cgcaatcggc aacagacaac
     3061 tggccagtag cgccggcacg cggagcagcg gttcgttcgg gcctgtgccg ccgttttcgt
     3121 tgtgcggtcc cagcgaatag gtcaacttcg ccagcaccgt gtgcagcacg tggttgttcg
     3181 ggacataatc gcccggctgg gccgccacca cgtgttgcca cggctgcagc acgtagtcca
     3241 cgagcgtcac catctcatcg aaccaaagcc cctgcgacag gtgcggccag cgcaccgcaa
     3301 gcgccaggag aaagacgccg agcgatgccc ataggatgcg cgaggttgaa ggtgatgaca
     3361 tcgcggttgc cgccgagccc tgaatggatg cgtcatcctg tggtgtgaac ggctgcgcgt
     3421 ctagtggtcg cccgattcgc cctgggcgtc gcgaaagttc tggtcttgcc gggcctcgta
     3481 ctcatcttca cgccgcatct cttcgaggca ttcgatgaaa tcggcgtgca cggacggcgc
     3541 aaggcgctgc tgaaatgatt cgtccagcgc gttggcacgg atcaaattca ccacgtctgc
     3601 gaagtcctga tatcgaccgg ccgcgagttt caactgcacg agcgtcggca gatcgacaac
     3661 ccgaagatcg cccacggttt gaccgacggt ggccggatcg ggaaaggaaa tgggtccagg
     3721 ctgaccgctg ccggggtatt ggcccgtgac gaggatgtcg aacgtgacgc cgttggcagc
     3781 atcgacaaaa cgccgcgggc gtcccggcac cgtgctgaac tcaccgctca tcaattgtcg
     3841 aaacgccgcg aaacccgcgg cgctcagcag aaaatcgacg tccttggttg tgcgttcatg
     3901 atgatgggca ttgacggcca tcccaccaac gatcgcgtaa gagattccag cg
//
LOCUS       DATIMH010000508         1830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2717523, whole genome shotgun sequence.
ACCESSION   DATIMH010000508 DATIMH010000000
VERSION     DATIMH010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2717523"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VLI90_08900"
     CDS             complement(<1..1305)
                     /locus_tag="VLI90_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV14364.1"
                     /translation="MRIAPWASRHARSILFMLAALVAGGVLGTLSLPVSLFPHVNFPR
                     IRVNLDAGERPAERMAVEVTRPVESAVRAIPGVRNIRSTTSRGSAEVAIAFDWGQDMT
                     AAFLQAQAEINRILPTLPTGTTFEVRRMDPTVFPVIAYSVTSKTHSLTELHDIAQYQL
                     MPILSTVPGVAKIGVDGGAIEEYRVVVDPAKLRARNVGINEVTAALSAANILNAVGRV
                     EDHFKLYLVVTDTRFKSIDDVGATVIRSGAGGVTLLSDIADIRHDRTPQFTHATADGR
                     DAVLINVFQQPGGNTVQIAAAIRQTLKDEQQHLPKDIAIASWYDQSDLITQSATSVRD
                     AVLLGVALAALVLLLFLRNLRMTLIAALAVPLVLAITALLLYVLNQSFNIMTLGGMAA
                     AVGLIIDDAIVMSEHIVRRLHGNVEHPVGATPASPGSATGDAG"
     gene            complement(1388..>1830)
                     /locus_tag="VLI90_08905"
     CDS             complement(1388..>1830)
                     /locus_tag="VLI90_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14365.1"
                     /translation="RIKPDQSVELFPISAGAGEDEDEEPIAAKIRLITQRVNPDTRLV
                     DVFVTPDTPAGLLLDSSLRATLTAEGRQGLIVPRDAVLPDEDGLLLFTVQDGKAVKHV
                     VKQGLKNDKEVEVIGEGLKAGDTVVIVGSLELEDGMAVTTEAPQ"
BASE COUNT          289 a          607 c          611 g          323 t
ORIGIN      
        1 gcctgcgtcg cccgttgccg atccaggcga cgcaggcgtc gcccccacag gatgttccac
       61 gttcccatgc aaccgccgga cgatgtgctc cgacatcacg atcgcatcgt cgatgatcaa
      121 ccccaccgcg gcggccatgc cgccgagcgt catgatgttg aagctctggt tcagcacgta
      181 cagcagcagc gcggtgatcg ccagcaccag cggcaccgcc agtgccgcga tcagcgtcat
      241 gcgcaggttg cgcaagaaca gcagcagcac cagcgccgcc agcgccacgc cgagcagcac
      301 ggcatcgcgc acgctggtcg cggactgcgt gatcaagtcg ctctggtcgt accagctggc
      361 gatcgcgatg tccttcggca gatgttgctg ctcgtccttc agcgtttgac ggatcgccgc
      421 cgcgatctgc acggtgttcc cgccgggttg ctggaagaca ttgatcagca ccgcgtcgcg
      481 gccatcggcg gtggcgtggg tgaactgagg cgtccgatca tggcggatgt ccgcgatgtc
      541 gctcagcagt gtcacgccgc cggcgccgga gcggatcacc gtcgcgccga cgtcgtcgat
      601 cgacttgaac cgcgtgtcgg tcacgaccag gtacagcttg aagtgatcct ccacccgccc
      661 caccgcgttc aggatattgg ctgccgacag cgcggcggtc acctcgttga tgccgacgtt
      721 gcgggcgcgc agctttgccg gatcgacgac gacgcggtac tcctcgatcg ccccgccatc
      781 gacgccgatc ttggcgacgc cggggaccgt cgagaggatc ggcatcagct gatactgcgc
      841 gatgtcgtgc agctcggtca gcgagtgggt tttcgaagtg acgctatacg cgatgacggg
      901 aaagacggtc gggtccatcc ggcgcacttc gaacgtcgtg ccggtcggca gcgtcggcag
      961 gatgcggttg atctccgcct gcgcctgcag aaacgcggcg gtcatgtcct ggccccagtc
     1021 gaaggcgatg gcgacttccg cgctgccccg gctggtggtc gagcggatgt tgcgcacgcc
     1081 ggggatggcg cggacagcgc tttcaacggg tcgagtgact tcgacggcca tgcgctcggc
     1141 cggtcgttcc ccggcgtcga ggttcacgcg gatgcgcggg aagttgacgt gcgggaagag
     1201 tgagacggga agcgacagcg tgccgagcac gccgccggcg acaagcgcgg cgagcatgaa
     1261 caggatggac cgcgcatggc gggaggccca gggagcgatt ctcaccgcgc ctccgcgcgt
     1321 tggtgcgctt gggggacaga cccactgaag tgggtccgcc gcgcgggttg tttcggcgca
     1381 gcgcggttca ttgcggggcc tccgtggtca cggccatccc gtcttcgagt tcgaggctgc
     1441 cgacgatcac cacggtgtcg ccggctttga gcccttcgcc gatcacttcc acttccttgt
     1501 cgttcttcag gccctgcttc acgacgtgct tcaccgcttt gccatcctgc acggtgaaca
     1561 gcagcaagcc atcttcgtcc ggcagcaccg cgtcgcgcgg gacgatgagc ccctgccggc
     1621 cctcggccgt cagcgtcgcc cgcagcgatg aatcgagcag caacccggcc ggcgtatccg
     1681 gcgtcacgaa gacgtccacc agccgcgtgt cggggttcac gcgctgcgtg atcagccgga
     1741 tcttcgcggc aatcggctct tcgtcttcgt cctcaccagc gccggcggag atcgggaata
     1801 actcgactga ttgatctggt ttgatgcgcg
//
LOCUS       DATIMH010000509         6939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2718495, whole genome shotgun sequence.
ACCESSION   DATIMH010000509 DATIMH010000000
VERSION     DATIMH010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2718495"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..1495
                     /locus_tag="VLI90_08910"
     CDS             485..1495
                     /locus_tag="VLI90_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HSV14366.1"
                     /translation="MSPALLRLLCLPRRRRTRSSGAFTLVELLVVIGIIAVLIGILLP
                     ALNKARRSSKTLQCSSNMKQIATAMLMYVNDNKGRLPPTLISAGDLTVYKDGWFWAAE
                     LMHQKYINAPNIYPPGATSSQKDPDQPSVFRCPEGIAPTDWPGASGTGSASNGAAPTD
                     AGNNAWVYGVAANPRADGQTPYGVATWYQLVTRVSGFASDDPISGTNNPPFIYFDSSK
                     NGVPPATAPGMAGQLQCPAYSRTISNIRKSAVVVMLGEADAINWVDNGDHPRNGHDQY
                     AVRMGARHGQISSDKNNAYMNLAFFDGHVALFPSQDVNYTVGGTAAMREANGMIFILH
                     NQ"
     gene            complement(1746..4844)
                     /locus_tag="VLI90_08915"
     CDS             complement(1746..4844)
                     /locus_tag="VLI90_08915"
                     /inference="COORDINATES: protein motif:HMM:NF033617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HSV14367.1"
                     /translation="MSLSTPFIHRPVGTTLLTIAIALAGAVAYSLLPVAPLPQVEFPT
                     IQVNAGLPGASPETVASSIATPLERQFGRIAGITEMSSNSSLGSTSITLQFDLSRNID
                     AAARDVQAAINAAAGQLPANLPRRPSFYKVNPSDAPIMILAIRSDLYDKGRMYDAAAT
                     VLQQKLSQVQGVGQVNIGGGSLPAVRVEVNPTALNGIGLSLEDVRSVLSTANANRPKG
                     SLADDTRTFSLNTTDQLLKASQYQPLIIEYKNGSAVRLSSIADVVDSVEDTRAVGIAN
                     GKPSILLFIFRQPGANIIATVDRVRALLPQLNASIPAAMNLSVVMDRTTTIRASVHDV
                     EITLLISIGLVILVVFFFLRDVRSTLIPSVAVPISLIGTFGVMWLCGYSIDNLSLMAM
                     TIATGFVVDDAIVVVENITRHLEQGMRPLPAALLGAKEIGFTVLSISVSLVAVFIPIL
                     LMGGIVGRLYREFAVTLSVAIGVSLIISLTTTPMMCALLLRAKDEQRHGRLFRASEAM
                     FQWILDRYEQSLAWVLRHQFLMILVTLATIGFTIYLYVIIPKGFFPQQDTSRLSAAIQ
                     ADQDTSFQATGRTVTRFAEQVAKDPAVENVIAFAGGSANSGRMFITLKPIEQRPSIDQ
                     VMARLRRNLAHIPGATLFPQAVQELKIGGRGSAALYQFSLQGDDVRELNEWAPKILER
                     MRTLRALTDVNSDQQNHGLQASLVIDRDTAARLGITPQVIDDTLYDAFGQRQVSTMYS
                     QLNQYHVVMEADPRFTQGPEGLQYVYVRSKTGTLVPLSAFTHYEPSVTPLQVNHSGQF
                     PSITMSFNLAPGHSLSDAVSQITDATREMGLPQTIHGTFSGTALAYQDSLSSQPYLIA
                     AALLAVYIVLGVLYESLIHPITILSTLPSAGVGALLALLMFHTELSVIALIGIILLIG
                     IVKKNAIMMIDFALAAERGEGLSPADAIFRACLLRFRPIMMTTMAALLGGLPLALGTG
                     TGAELRRPLGIAIVGGLLFSQVLTLYTTPVVYLYLDRLRLWLNSRRHSAAMHLPPLAP
                     AQ"
     gene            complement(4951..>6939)
                     /locus_tag="VLI90_08920"
     CDS             complement(4951..>6939)
                     /locus_tag="VLI90_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV14368.1"
                     /translation="YSLNNLSLMALTISTGFVVDDAIVMIENIVRYIEAGENPLAAAL
                     KGSEQIGFTILSLSVSLIAVLIPLLFMGDIVGRLFREFAVTLSVTILVSAVVSLTLTP
                     MMCAKLLRHQPEAENGWFYRVSERAFQATIDLYGRTLQVVLRHQFLTLLVAIATLALT
                     VVLYIYIPKGFFPVQDTGVILGISEAPQSVSFKSMSQRQLALADVILKDPAVDSLSSF
                     IGIDGTNPTLNNGRIQINLKPIEQRGISASDVIRRLQPELAQVPGITLFMQPVQDLTV
                     EDRVSRTQFQYTLEDPDPKELAQYVPPFMAKLQELPELRDVASDQQNDGLEVRLVIDR
                     DTASRLGITPQMLDDTLYDAFGQRQASTMYTQLNQYHVVLEVQPRFRRSPRDLDHIYV
                     HPAGGGEVPLSAFCRFEDQSAPLTINHQGQFPVVTVSFNLAPHASLGEAVRAVNAAKD
                     SLDMPLSIQAGFQGTASAFQASLTNTPVLILAALITVYIVLGVLYESYIHPITILSTL
                     PSAGVGALLALMLCGNDFSIIALIGLILLIGIVKKNGIMIIDFALDAQRQQGKSAVDA
                     IYEACLLRLRPILMTTFAALFGGVPLALGTGYGAELRRPLGIAMIGGLLFSQVLTLYT
                     TPVIYLYFDRLAARVSELVSRPREDAVLIPSPGMPGEG"
BASE COUNT         1286 a         2110 c         2321 g         1222 t
ORIGIN      
        1 ccgacaaccg cttgggttgc tacgtcacgg agacgatctt cttcctgaac agacagtggg
       61 gcggaaaata atctgactgg gcaagtgacc acgggcggtt ccacctaagc catcaaggga
      121 cgcacaagat cgcatcttgt gcgtcctttt tttcgtctgc ggccggtcga aatgaatggc
      181 ttagaaggga gccgtttcct acaggggtgc cgccgaaaaa tagcattatt ctctgcgctt
      241 ttatccactt cgcgatccac gcagactgtt gccgtgaatc cgcagcccca gccgtttttc
      301 agtattttcg gttgcacacc tgccgtttca ggcgtatatt ccgcgaatcg tcgtcaccgc
      361 catgttggtg aatagacggt aaaaataaat cagttaatcg agatcatttc tcatcagttg
      421 ttccgtcgga ttctcgttcc ccgcggggat ttttgacggc cgaaaaccga acggaggtac
      481 ccgcatgtct cccgcgctcc tgcgtctcct gtgtctcccg cggcgtcgtc gcacccgaag
      541 ctctggcgcg tttacgctgg tcgagctgct ggtggtcatc ggcatcatcg cggtgctgat
      601 cggcatcctg ctgccggcgc tgaacaaggc ccggcggtcg tccaagacgc tgcagtgctc
      661 gagcaacatg aagcagatcg cgaccgcgat gctgatgtac gtcaacgaca acaagggccg
      721 gctgccgcca acgctgatca gcgcgggcga cctgacggtg tacaaggacg gctggttctg
      781 ggcggcggag ctgatgcacc agaagtacat caatgccccg aacatctacc cgccgggcgc
      841 gacatcgagc cagaaggacc cggatcaacc cagtgtgttc cgctgccccg aggggattgc
      901 gccgacggat tggcccggcg ccagcggcac cgggtcggcc agcaacggcg ccgcgccaac
      961 cgacgcgggg aacaacgcat gggtctatgg cgtggcggcc aacccgcgcg ctgacgggca
     1021 gacgccgtat ggcgtcgcca cgtggtatca actggtcacc cgcgtcagcg gattcgccag
     1081 cgatgacccg atcagcggga cgaacaatcc gccgttcatc tacttcgact cgtcgaagaa
     1141 cggcgtgccg ccggccaccg cgccaggcat ggcgggtcaa ctgcagtgcc cggcgtattc
     1201 gcgcacgatc agcaacatcc gcaagagcgc ggtggtggtg atgctgggcg aggcggacgc
     1261 catcaactgg gtcgacaacg gcgaccatcc gcgcaacggt cacgaccaat acgcagtgcg
     1321 catgggcgcg cggcatgggc agatcagctc cgacaagaac aacgcttaca tgaacctggc
     1381 gtttttcgac gggcacgtcg cgctattccc gagccaggat gtgaattaca cagtgggcgg
     1441 cacggcggcg atgcgggaag cgaatgggat gatcttcatt ctgcacaatc agtgaagtgt
     1501 gtttgccttc gggtgcaatc gacggggacg cgcaagagct tcttgtgcgt ccctttttct
     1561 tttgctttgt agctgcgacg cctgcgtcgc cttttctggg atacggagat tcccgagaaa
     1621 aagaggcgat gcaggcgtcg cagctacggt gatgccgaag aaggctctgc cctgggcaga
     1681 tattccgaag ctgtgtccca cggcagagcc gtgcgccttt tttcgtggcg cgtgagcgcg
     1741 cgacttcatt gggccggcgc cagcgggggg aggtgcatcg ccgcgctgtg acggcggctg
     1801 ttgagccaca ggcgcaggcg atcgaggtac aggtacacca ccggcgttgt gtacagcgtg
     1861 aggacctggc tgaagagcag cccgccgacg atggcgatgc cgagcgggcg gcggagttcg
     1921 gcgccggtgc cggtgcccag cgccaggggc aggccaccca ggagcgcggc catcgtcgtc
     1981 atcatgatgg ggcggaagcg cagcaggcag gcgcggaaga tcgcatcggc gggcgagagt
     2041 ccttcgccgc gttcggcggc gagggcgaag tcgatcatca tgatcgcgtt cttcttcacg
     2101 atgccgatca gcaggatgat gccgatcagc gcgatcacgc tcagttccgt gtggaacatc
     2161 agcagcgcca gcagcgcccc cacgccggcc gacggcagcg tcgagaggat cgtgattggg
     2221 tggatcaggc tttcgtacag cacgccgagc acgatgtaga ccgccaagag cgccgcggcg
     2281 atgagatacg gctggctcga gagcgaatcc tggtacgcca gcgccgtgcc ggagaacgtg
     2341 ccgtggatcg tctgcggcag gcccatctca cgcgtggcgt cggtgatctg gctgaccgcg
     2401 tcgctcagcg aatggccggg cgcgaggttg aacgacatgg tgatcgacgg gaactgcccc
     2461 gagtggttca cctgcagcgg cgtgaccgag ggctcgtaat gggtgaacgc gctgagcggc
     2521 acgagcgtgc cggtcttcga tcgcacgtac acgtactgca aaccctcggg cccctgcgtg
     2581 aagcgcgggt cggcctccat taccacgtgg tactggttca gctgcgaata catggtcgag
     2641 acctgccgct ggccgaacgc gtcgtacagc gtgtcgtcaa tgacctgcgg cgtgatgccg
     2701 aggcgcgcgg cggtgtcgcg atcgatgacc agcgacgcct ggagcccgtg gttctgctgg
     2761 tcggaattga cgtcggtcaa cgcgcgcagc gtgcgcatgc gctcgaggat cttcggcgcc
     2821 cattcgttca actcgcgcac gtcgtcgccc tgcaatgaga actgatacag cgccgccgag
     2881 ccgcgcccgc cgatcttcaa ttcctgcacc gcctgtggga acagcgtggc gccggggatg
     2941 tgcgccaggt tccggcgaag gcgggccatc acctggtcaa tcgacggccg ctgctcgatc
     3001 ggcttgagcg tgatgaacat gcggccggaa ttggccgagc cgccggcgaa ggcaatcacg
     3061 ttttccaccg ccgggtcctt cgcgacctgt tcggcgaacc gcgtgaccgt gcgaccggtg
     3121 gcctggaacg aggtgtcctg atccgcctga atggcggccg agagccggct ggtgtcctgc
     3181 tgcgggaaaa atcccttggg gatgatgacg tacaggtaaa tcgtgaagcc gatcgtcgcc
     3241 agcgtgacga ggatcatcag gaactgatgc cgcagcaccc acgccagcga ttgttcgtag
     3301 cggtcgagga tccactggaa catcgcctcg ctggcgcgga acagccggcc gtggcgctgt
     3361 tcgtctttgg cgcggagcag cagcgcgcac atcatcgggg tggtggtgag cgagatgatc
     3421 agcgacacac cgatggcgac cgacaacgtc acggcgaact cgcggtacaa gcgcccgacg
     3481 atgccgccca tcagcaggat ggggatgaac accgcgacga gcgagacgct gatcgacagg
     3541 acggtgaagc cgatttcctt tgcccccagc aacgccgccg gcagcgggcg catgccttgt
     3601 tcgaggtgcc gcgtgatgtt ttcgaccacg acgatcgcgt catcgacgac gaaaccggtg
     3661 gcgatggtca tcgccatcaa tgagaggttg tcgatgctgt acccgcacag ccacatcacg
     3721 ccgaacgtgc cgatgagcga aatgggcacg gccacgctgg ggatgagcgt ggatcgcacg
     3781 tcgcgcagga agaagaagac gacgaggatg accagcccga tcgagatcag cagcgtgatt
     3841 tcgacatcgt gcaccgacgc gcggatggtg gtcgtgcggt ccatgacgac cgacaaattc
     3901 atggccgcgg ggatcgacgc gttcagctgc ggcagcaacg cgcgcacgcg atcgacggtg
     3961 gcgatgatgt tcgcgccggg ctggcggaag atgaacagca ggatggacgg tttgccgttg
     4021 gcgatgccga cggcccgggt gtcttccacc gaatcgacaa cgtcggcgat ggacgacagc
     4081 cgcacggccg acccgttctt gtactcgatg atgagcggtt gatattggct ggccttgagc
     4141 agctgatcgg tggtgttgag cgagaacgtg cgcgtgtcat cggccagcga tcccttgggg
     4201 cgattggcgt tggcggtgct cagcaccgag cgcacgtctt cgaggctcag gccgatgccg
     4261 ttcagcgcgg tcgggttcac ctcgacgcgc acggccggca gcgaaccgcc gccgatgttc
     4321 acctgcccca ccccttgcac ctgcgacagc ttctgctgca gcaccgtggc ggcggcgtcg
     4381 tacatgcggc ccttgtcgta caggtccgag cggatcgcca ggatcatgat cggcgcatcg
     4441 gatggattga ccttgtagaa gctaggccgc cgcggcaagt tcgccggcaa ctgccccgcc
     4501 gcggcgttga tggcggcctg cacgtcgcgc gcggcggcgt cgatgttgcg gctcaggtcg
     4561 aactgcagcg tgatgctcgt cgagcccagc gaactgttcg acgacatctc ggtgatgccg
     4621 gcgatgcgcc caaactgccg ctccagcggc gtggcgatgg acgaggcgac cgtctccgga
     4681 ctcgcccccg gcagccccgc gttgacttga atcgtcggaa actccacctg cggcaatggc
     4741 gccaccggca gcagcgagta cgcaaccgcg ccggccagcg cgatggcgat cgtcagcaac
     4801 gtggtgccga ccgggcggtg gatgaagggg gtggagaggc tcaatgcagc ccttcctctc
     4861 cggccccctc tcccggtacg ccgggagagg gttggggtga gggtgatttt gtgaatcgat
     4921 ggtcactcga catccgaaat caccctcacc ctagccctct cccggcatac cgggagaggg
     4981 gatcagaacg gcatcttcac gggggcgact tacaagctcg ctcaccctcg ccgcgagtcg
     5041 atcgaagtaa agataaatca ccggcgtcgt gtacagcgtc aggacctggc tgaacagcaa
     5101 gccgccgatc atcgcgatgc cgagggggcg gcgcagctcg gcgccgtaac cggtgcccag
     5161 cgccagcggc acccctccga ataatgcggc gaatgtggtc atcaggatcg gccgcaggcg
     5221 gaggaggcag gcttcgtaga tcgcgtccac cgcgctcttc ccctgctggc gctgcgcgtc
     5281 cagcgcgaag tcgatgatca tgatgccgtt cttcttcacg atgccgatca gcaggatcag
     5341 gccgatcagc gcgatgatgc tgaagtcgtt gccgcagagc atcagcgcca acagcgcccc
     5401 gacgccggcg ctgggcagcg tcgagagaat cgtgatcggg tggatgtaac tttcgtacag
     5461 cacgccaagg acgatgtaga cggtgatcag cgccgccagg atgagcaccg gcgtgttggt
     5521 gagcgatgcc tggaacgccg acgcggtgcc ttggaaaccg gcctggatgc tgagcggcat
     5581 gtccagcgaa tccttggccg cgttcaccgc gcgcaccgct tcgccgagcg acgcgtgcgg
     5641 cgcgaggttg aaggagaccg tgacgaccgg gaactgcccc tggtggttga tcgtcagcgg
     5701 cgcgctttga tcttcgaacc ggcagaacgc gctgagcggc acctcgccgc caccggcggg
     5761 atgcacgtaa atgtgatcca gatcgcgcgg gctgcggcgg aagcgcggct gaacctccag
     5821 caccacgtgg tactggttca gctgcgtgta catcgtcgag gcctgccgct ggccaaacgc
     5881 atcgtacagc gtgtcgtcca gcatctgcgg ggtgataccg aggcgcgacg cggtgtcgcg
     5941 atcgatcacc agccggactt ccagcccgtc gttttgctga tcgctggcga cgtcgcgcag
     6001 ctccggcagt tcctgcagct tggccatgaa cggcggcacg tactgcgcca actccttcgg
     6061 gtccgggtct tccagcgtgt attgaaactg cgttcggctg acgcggtctt ccacggtcag
     6121 gtcctgcacc ggctgcatga aaagggtgat gcccggcacc tgcgccagct ccggttgcag
     6181 ccggcgaatc acgtcgctgg cgctgatgcc gcgctgctcg atcggcttga ggttgatctg
     6241 gatgcggccg ttgttcagcg tcgggttcgt gccgtcgatg ccgatgaacg acgacaggct
     6301 atcgaccgcc gggtctttca agatcacgtc ggccagcgcc agctggcgct ggctcatcga
     6361 cttgaacgag acgctctgcg gcgcttcgga aatcccgagg atcacgccgg tgtcctgcac
     6421 ggggaagaag cccttgggga tgtagatgta cagcacgacg gtgagcgcga gcgtggcgat
     6481 ggcgaccagc agcgtgagga actgatgccg cagcacgacc tgaagcgtgc ggccgtacag
     6541 gtcgatcgtc gcctggaacg cgcgctcgct gacgcggtag aaccagccgt tttccgcttc
     6601 gggttgatgg cgcagcagct tggcgcacat catcggcgtg agcgtgagcg agacgacggc
     6661 agagacgagg atcgtcacgc tgagcgtcac cgcgaattcg cggaacagcc ggccgacgat
     6721 atcgcccatg aacagcagcg ggatgagcac cgcgatcagc gaaacgctca gcgacagaat
     6781 ggtgaagccg atctgttcag agcctttgag tgccgcggcg agcgggttct cgccggcttc
     6841 gatgtagcgg acgatgtttt cgatcatgac gatcgcgtcg tctacaacga aaccggtgga
     6901 gatcgtcagc gccatcaaac tcaggttgtt gaggctgta
//
LOCUS       DATIMH010000510         6013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_701419, whole genome shotgun sequence.
ACCESSION   DATIMH010000510 DATIMH010000000
VERSION     DATIMH010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701419"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2342)
                     /locus_tag="VLI90_08925"
     CDS             complement(<1..2342)
                     /locus_tag="VLI90_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14369.1"
                     /translation="MSGHAKRMVQSARRRRRGAIEAVAAAAATSIALSAPIASATSGY
                     YVGPSNGNWNSAANWAATSGGAGGVGVPGDGDFAVLDSPANTTVNFDGSFSTGLSTFW
                     IGSSGAANPTLNIAAGTVRYNNVADFYGMLLGKGGQGTISLSGTGALNVTGNEEVGWD
                     NTGTFIQSGGTHTVDNSHAMLLGRFAAASGSYLLSGGSLNAGSEYVGYDGSGTFTQTG
                     GTNTVYANLFIAGDALGSSGSYTISNGTLLADFIGVSGGVNDPGGTAALTVNSGGHVT
                     LTATPLNTAVSVLRLFGGGTVTVNTGGLITGGSVWDDGTFNFNGGSATFASGANVGLT
                     NTGVFNQTVAGTTLVNGGNLQVGSNTGANGTYTLAGAGTVVSVSLAEYIGNGGAGTFN
                     QSGGSNSLALELRIGEQASGNGTYNLSAGSVTAGESEFIANLGVASFNQTGGTNNANG
                     LRVASGVGSIASYSLGGTGSVSITGASAFENIGESGLGIFNQSAGTHSVSGAVAISQY
                     AGSRGIYNLSGGTYSISSDLNIGGQGIGTNANLNISGGAISVTGTAGIGSSTGNATLS
                     QSAGTSTVGQLIVGGSAGSAGVVLLSGGALNGGVVENIGSSGSAIFNQTGGTHNTSEI
                     FLSANGTNNSAYLMSGGNLNVNVAGNPNNLYVGGSAGASFVQSGGAVTVGATLDLAHT
                     AGEHGSYTLSGAGSLSAASEYIGDTGVGTFVQSGGTNTTQFIDLSAVNGSDASTYRLS
                     GGTLLVNSATANVFIGDFASGSFIQSGGTFSQTNNGLYLGR"
     gene            complement(2667..5732)
                     /locus_tag="VLI90_08930"
     CDS             complement(2667..5732)
                     /locus_tag="VLI90_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HSV14370.1"
                     /translation="MTLFEPLERRQLLSVSVNVATGLLTVTGTNLADLIRVERSGKNL
                     AIHEGRKTTNVALSKVTGIVINGGKGNDSLSVEQNVKVPATLNGEAGNDVLVSGGGAD
                     VLSGGDGIDTADYSGRTRALTLVIGGGPVSGESGEHDNILGDVENLIGGRGSDSLVGS
                     AAANVIDAGRGDDSITGNGGNDLLIGGRDDDTYIFTPTTDAQLVKVVEVTLGGDDTLD
                     FSALDASMAVTALLQAKPGANLATCANTQVQNAGQTGDLENVLGGAGDDNITGHNGKN
                     RLVGNGGNDSIYGLGGNDYIDGGEGDDFLDGGTGADDIHGGGGSDTVDHSTRTNGVNV
                     SADDTANDGEAGESDNVHSDVEEIVGGDGNDSLSGAATGSILVGGNGKNILTGGAGAD
                     VIFGGPNGDMIVGNGGADVVLCGNGNDTVFTGDSSGDPLATGPDTGGASVFAGGGDDS
                     IVGGNGADFLDGQSGNDTVHGGGGNDSVYGEDGNDYLFGDAGNDFISGGIDNDSIGGG
                     TGQDTVDAGDGADLVQKNLPDPVTGVVATPLRAVVAPTPVVVVVNSDDTDPPIPDPDP
                     PSTPTDPGPGDSVDGGTGNDTLIGGPFDDVLNGGDGADSIQGGDGNDTLNGQDGDNQI
                     FGEGGDDLISTGIGNSTLDGGDGADFFVNAQGLADRVDGGAGYNIAEFDPAGTDTLLN
                     IQLISNPQEVILQVAPGQATPATSPVDGAFGPFTNVANDSITPVVTGQAVLDGGGNLV
                     IDDSLATVKHTVIITQSGTNLVANDNGVITTVPFAAVTGGVIITGGSGDDYFTANNVP
                     VRTEMFGNDGNDTLIGGSYDDGHGTLGDALFGGNGSDLIQGMNGPDNLDGDAGNDSLY
                     GGPGNDTLDGGISGGFYAGGDGRDLLSGGTGNDYANYSTRTDNLVLRLDGTARSGQAG
                     SVENDTIMTDVENLLGGTGNDVLVGNALANYISGGNGNDTIFGAGGGDAILGQGGADK
                     VYGGAAYDFLYISGDGNADGYNIGGATAEFVQKDTSPADFVTADLAPPA"
BASE COUNT          919 a         2090 c         1858 g         1146 t
ORIGIN      
        1 cggcccaggt agagtccgtt gttggtctgg ctgaacgtgc caccactttg gatgaaggat
       61 ccggaggcga aatcaccgat gaaaacgttc gccgtagccg agttaaccag caaagttccg
      121 ccgctcagcc gataggtcga agcatccgaa ccgttaacgg cggaaaggtc gatgaactga
      181 gtggtgttgg tcccaccgct ctggacgaac gtgccgacgc ccgtgtcgcc gatgtactct
      241 gatgcggcgc tcagcgaacc ggcgccgctg agcgtatagc tgccgtgctc cccggcggtg
      301 tgggccaaat ccaacgtagc gccaacggtg acggccccac cgctctgcac gaacgacgcg
      361 ccggcgctgc caccgacgta caggttgttc ggattcccgg cgacgttcac gttcaagtta
      421 ccgccggaca tcaggtacgc actgttgttc gtgccgttgg cgctgaggaa gatctcagat
      481 gtgttatgcg tgccgccggt ctggttgaaa atcgccgagc cggacgagcc gatgttttcg
      541 acgaccccgc cgttgagcgc gccgccgctg agcagcacga cgccggcgct gcccgcactg
      601 ccgccgacga tcaattgccc caccgtgctg gtgccggcgc tttggctgag cgtcgcgttg
      661 ccggtgctgc tgccgatgcc tgcggtgccg gtgacgctga tcgccccgcc gctgatgttc
      721 aagttcgcgt tggtgccgat gccctgcccg ccgatgttca ggtctgagct gatgctgtac
      781 gtcccgccgc tgaggttgta gatgccgcgg ctgccggcgt attgcgagat cgcaaccgcg
      841 ccgctgacgc tgtgcgtgcc agcgctttgg ttgaagatgc cgaggccgct ttcgccgatg
      901 ttttcaaacg cgctcgcgcc ggtgatcgac acgctgccgg tgccgcccag gctgtagctg
      961 gcgatcgacc cgacgccgct ggcgacgcgc aggccgttgg cgttgttcgt gccgcccgtc
     1021 tggttgaacg atgcgacgcc gaggtttgcg atgaattcgc tctcgccagc ggtcacactg
     1081 cccgcgctga ggttgtaggt gccgttgccg ctcgcctgct cgccaatccg cagctccagc
     1141 gccaggctgt tcgagccgcc gctttggttg aacgtgccgg ccccgccgtt gccgatgtat
     1201 tccgccagcg acaccgagac cacagtgcca gcgccagcca gcgtataggt gccgttcgcg
     1261 ccggtgttcg agcccacctg aaggttcccg ccgtttacca gcgtggtccc cgccaccgtc
     1321 tgattgaaga cgcccgtgtt ggtcagcccg acattcgcgc cgctggcgaa cgtggcgctc
     1381 ccgccattaa agttgaacgt gccgtcgtcc cacacgctgc cgccggtgat caagccgccg
     1441 gtgttgacgg tgacggtccc gccgccgaac aggcgcagga cgctcacggc ggtgttgagc
     1501 ggcgtcgccg tcagcgtcac gtgaccaccg ctgttgacgg tcagcgccgc cgtgccaccg
     1561 ggatcgttca cgcccccgct gacaccgatg aaatccgcca ggagggtgcc gttgctgatc
     1621 gtgtagctgc cgctcgatcc aagcgcgtcg ccggcgatga agaggttggc gtagactgtg
     1681 ttagtcccac cggtctgcgt gaacgtgccg ctgccgtcgt agccgacgta ctcgctgccg
     1741 gcgttgaggc tcccgccact gaggagatag gacccgctcg cggcggcgaa gcgcccgagc
     1801 agcatcgcgt ggctgttgtc caccgtgtgc gtgccgccgg actggatgaa cgtgccggtg
     1861 ttgtcccaac cgacttcttc gttgccggtg acattgagcg cgccggtgcc actgaggctg
     1921 atcgtgccct gcccgccctt gcccagaagc atgccgtaga aatcggcgac gttgttgtag
     1981 cgcaccgtgc cggcggcgat gttgagcgtg ggattggccg ccccgctgct gccgatccag
     2041 aatgtgctca agcccgtgga aaagctgccg tcgaagttga cggtggtgtt ggcaggtgaa
     2101 tccagcacgg cgaagtcgcc gtcgccgggc acgccgacgc cgccggcgcc gcccgacgtc
     2161 gccgcccagt tggcggcgct gttccagttg ccgttactgg gcccgacgta atatccgctg
     2221 gttgcggacg cgatgggcgc cgatagcgcg atagacgtcg ccgcggccgc ggccaccgcc
     2281 tcgatcgctc ctcgccgccg ccgtcgcgcc gactggacca ttcgtttcgc gtggccgctc
     2341 atcggttgtc cttccaccac ccactcccgc aaacccaatc agcgcatcgc ggttcaccgc
     2401 ggcgcgcttg tctgtcatcc tccagcggag gcgtactcgc cggattcgga ggatctagac
     2461 tagaagccgc tgacccaaca ctcgcgcaaa tcgccccgca gtcgcttgcg gcttcgcaga
     2521 cggacaaacc gcggtgctcg atctgcgaaa ccgcaagcga ccgccgcacc gctctcgcat
     2581 ctaaacgttc acttacttcg cagcacgatc cttcgtctgc gcacgcgtac gtgctgcgcc
     2641 ggaggatgac actcgcgcgc agctgatcat gccggcggcg ccagatccgc cgtcacgaag
     2701 tcggccgggg aggtgtcctt ctgcacgaac tccgccgtcg ccccgccgat gttgtagcca
     2761 tccgcgttgc cgtcgccgct gatgtacagg aagtcatacg ccgccccgcc gtacaccttg
     2821 tcggcgccgc cctggccgag gatcgcatcg ccgccgccag cgccgaagat cgtgtcgttg
     2881 ccgttgccgc cggagatgta gttcgccagc gcgttgccga ccagcacgtc gttgccggtg
     2941 ccgccgagca ggttctcgac gtcggtcatg atcgtgtcgt tctccacgct gcccgcctgc
     3001 ccgctgcggg cggtgccgtc cagccgcagg accaggttgt cggtgcgtgt gctataattg
     3061 gcatagtcgt tgccagtccc gccgctgagc agatcgcgac catcgccacc ggcgtagaac
     3121 ccgccgctga tcccgccgtc cagcgtgtcg ttgcctggcc cgccgtagag cgagtcgttg
     3181 cccgcgtcac catccaggtt gtccgggccg ttcatgcctt ggataagatc gctgccgttg
     3241 ccgccgaaca gcgcatcgcc gagcgtgccg tggccgtcgt cgtagctgcc gccgatcagc
     3301 gtgtcgttgc cgtcgttgcc gaacatctcg gtgcgcaccg ggacgttgtt ggcggtgaag
     3361 taatcgtcac ccgatccgcc agtgatgatc acgccgcccg tcaccgccgc gaagggaacg
     3421 gtcgtgatga cgccgttgtc gttagcgact agattcgtgc cgctctgcgt gataatgacc
     3481 gtatgcttca ctgtggccag cgaatcgtcg atgaccaggt tgccgccgcc gtccagcacc
     3541 gcctggcccg tgacaaccgg cgtaatcgaa tcgttcgcga cattcgtgaa cggcccgaac
     3601 gcgccgtcca ccggcgacgt ggccggcgtc gcctggcccg gcgccacctg aaggatgact
     3661 tcctgcggat ttgagatcag ctgaatgttc agtagcgtat ccgtgccggc cggatcgaac
     3721 tcggcaatgt tgtagcccgc gccaccatca acacggtccg ccaagccctg cgcgttgacg
     3781 aagaagtcag cgccgtcgcc gccatcgagc gtgctgttgc cgatgccggt ggagatcagg
     3841 tcatccccgc cctcaccgaa gatttgattg tcgccatcct ggccattcag cgtgtcgttg
     3901 ccatcgccgc cctgaatgct gtcggcgcca tcgccgccgt taagcacatc gtcgaacggc
     3961 ccgccgatga gcgtgtcatt gccggtgccg ccatcgaccg aatcaccggg acccggatcg
     4021 gtcggggtgg acggtggatc gggatccgga ataggcgggt ccgtatcatc cgaattcacc
     4081 acgacgacaa ccggcgtcgg cgcgacaacc gcccgcaacg gcgtcgccac cacgcccgtc
     4141 accgggtccg gcagattctt ctggacgagg tccgcgccgt cgccggcgtc caccgtgtcc
     4201 tgccccgtgc cgccgccaat tgaatcgttg tcgatcccac cgctgatgaa gtcattgccg
     4261 gcgtcgccga acaggtagtc gttgccgtcc tcgccataga cgctgtcgtt cccaccgccg
     4321 ccgtggacgg tgtcgttgcc gctttggccg tcaaggaaat ccgcgccgtt cccgccaacg
     4381 atcgagtcgt cgccgccccc ggcgaacaca gatgccccgc ccgtgtcggg cccggtagcc
     4441 aagggatcac cgctgctgtc gccggtgaac acggtgtcgt tcccgttgcc gcaaaggaca
     4501 acatccgccc cgccgttgcc gacgatcatg tcgccgttcg gcccgccgaa gatcacgtcg
     4561 gcgcccgccc cgccggtgag gatgttcttc ccattgccac cgacgaggat cgatccggtc
     4621 gcagcgccgc tcaagctgtc gttcccgtcg ccgccgacga tctcttcgac atcgctatga
     4681 acgttgtcgc tttcgccggc ttcgccgtcg ttggcggtgt cgtccgcgct gacgttcacg
     4741 ccgttggttc gcgtggagtg atcgaccgtg tcgctgccac cgccgccgtg gatgtcatcg
     4801 gcgccagtgc cgccgtctaa aaagtcgtcg ccttcaccac catcaatata gtcgttgccc
     4861 cccaggccat agatcgaatc gttcccgccg tttccgacca ggcggttctt gccgttgtgg
     4921 cccgtgatgt tgtcgtcgcc cgccccgccg aggacgtttt ccagatcgcc cgtctgcccc
     4981 gcgttctgca cttgcgtgtt ggcgcaggtc gccagattcg cacccggctt ggcttgcagc
     5041 aacgccgtca ccgccatgct ggcgtcgagc gcgctgaagt ccagcgtgtc atccccaccg
     5101 agcgtcacct cgacgacctt caccagctgc gcgtcggtcg tcggcgtgaa gatgtacgtg
     5161 tcgtcatccc gtccgccgat caacaggtcg ttcccaccgt tgccggtgat gctgtcatcc
     5221 ccgcggccgg cgtcgatcac attggcggcg gcgctgccca ccagcgagtc gctgccgcgc
     5281 ccgccgatca ggttctcgac gtcgcccaag atattgtcat gttccccgct ctcgccgctg
     5341 actggcccgc cgccgatgac cagcgtgagg gcgcgcgttc gccccgagta atccgccgtg
     5401 tcgatgccat cgccaccgga cagcacatcg gccccgccgc cggagacgag cacgtcattg
     5461 cccgcttcgc cgttcaatgt cgcgggaacc ttcacgttct gctcgacgct cagcgagtcg
     5521 ttgcccttgc cgccgttgat gacgatgccc gtgaccttcg acagcgcgac attggtggtc
     5581 tttcgccctt cgtggatcgc caggttcttc cccgaccgct ccacgcggat caggtccgcc
     5641 aggttcgtcc ccgtcacggt caacaaaccg gtggcgacgt tgaccgaaac ggacaacagc
     5701 tggcggcgtt ccagtggttc aaacaacgtc atggtactac cccggcaaat gcccaaccga
     5761 gccaggcgac cagcgggcgt tcctgccgcg cgcgaccgcc tgctgcacgg gtatctacgc
     5821 cagaccgcgc gcgatcctgt gtgatcccgg ggcaaaatgt gttcaaaagc aacaatggcg
     5881 cggcgactgc cgcggggcgc gattttctcc ctacgctttg aatcgacctg cgcttatgag
     5941 aaatgcaacc gccgcagatc gctggccctg attctctgct tacaaccggg acggcaatct
     6001 acgttaacgg gtt
//
LOCUS       DATIMH010000511         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3297792, whole genome shotgun sequence.
ACCESSION   DATIMH010000511 DATIMH010000000
VERSION     DATIMH010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3297792"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VLI90_08935"
     CDS             complement(<1..1363)
                     /locus_tag="VLI90_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HSV14371.1"
                     /translation="MERSNLTRWIYVCLAIGSWAFLLLALGSFHPTDWPSHAVHPYPP
                     IQNMCGPVGAFIAYYLFLIIGQGAFPMLFFSGVCVVLFVAHNRVSDPWMRAIGLLLLS
                     IAFAAAVHHFKPGSENGFPEGQGGVLGIGAASFLQAHFSTAGTRLVLLTAILIGMLLA
                     ADDLVLRAPVFVGAAVVTAKHATSTFDLKKLPSLSRMGAAGKDAAKGGAPVQRITKPD
                     EDPEDLKSPVMLRPWSNWLSRSKKDDVELTYENDEPHTPAAADAAFIDAADPAPAAHA
                     LIPPPPPVTPEIPVTPMAPAIAETSADAAAAPAPIEPPVQRKDIIVKLPSMLKPRQVS
                     PPPPPPKELGEYHLPQWDMLEDAEHGYAESQEAFVREKAAVLEQALKEFDVEAHVVEI
                     DTGPVITMYELALAAGVKVSAISALSNDIQRALRAETIRIVAPIPGKSTVGIEVPNEQ
                     KELV"
     gene            complement(1451..>1705)
                     /gene="dut"
                     /locus_tag="VLI90_08940"
     CDS             complement(1451..>1705)
                     /gene="dut"
                     /locus_tag="VLI90_08940"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HSV14372.1"
                     /translation="VRPRSGLSSKHGVTMINAPGTIDSDYRGEVHVPLINHGRQPFTI
                     ERGMRIAQMLILPVPAVEMVEVGSLDETARGKGGFGHTGH"
BASE COUNT          284 a          543 c          555 g          323 t
ORIGIN      
        1 ggactaattc cttctgctcg ttcgggactt cgatgccgac ggtgctcttg cccgggatcg
       61 gcgcgacgat gcggatcgtc tccgcgcgca gcgcccgctg gatgtcgttg gatagcgcgc
      121 tgatcgccga caccttcacg cccgccgcca gggccagctc gtacatcgtg atgaccgggc
      181 cggtgtcgat ctcgacgacg tgcgcttcaa cgtcgaattc tttcagcgcc tgctccagca
      241 ccgccgcctt ttcgcggacg aacgcttcct ggctttcggc gtagccgtgt tcggcgtctt
      301 ccaacatgtc ccactgcggc aggtggtatt cgcccagttc cttcggtggc gggggcggcg
      361 gcgagacctg ccgcggcttc aacatgctcg gaagcttgac gatgatgtcc ttgcgctgca
      421 ccggtggttc gattggcgca ggcgccgcgg cggcatcggc gcttgtctcg gcaatcgccg
      481 gcgccatcgg tgtcacggga atctccggcg tcaccggggg cggtgggggg atcagcgcgt
      541 gtgctgccgg cgcggggtcg gcggcatcga tgaacgccgc gtccgccgcc gcgggcgtgt
      601 gcggttcgtc gttctcgtac gtcaactcga cgtcgtcttt ctttgatcgc gacagccagt
      661 tggaccacgg ccgcaacatc accggcgact tcaagtcttc cggatcctcg tccggcttgg
      721 tgatgcgctg aaccggtgct ccgcccttgg cggcgtcctt gccagcggcg cccatcctcg
      781 acagcgacgg gagcttcttc agatcgaacg tagacgtcgc gtgcttggca gtcaccaccg
      841 cagcgccaac gaagaccggc gcgcgaagca ccagatcgtc cgcggccagc agcatgccga
      901 tcaggatggc ggtcagcagc accagtcgcg tgccggcggt gctgaagtgc gcctgcagga
      961 acgacgccgc cccgatgccg agcacgccgc cctggccttc ggggaatcca ttttcactgc
     1021 cgggtttgaa atgatgcacc gccgcggcga aggcgatcga cagcagcagc agcccgatcg
     1081 cccgcatcca cggatcgctc acgcgattgt gcgcgacgaa tagcacgacg cacacgccgc
     1141 tgaagaacag catcgggaac gcgccttggc cgatgatcag aaacaggtag taggcgatga
     1201 acgccccgac cgggccgcac atattctgga tcggcggata cggatgaacc gcatggctgg
     1261 gccaatcggt cggatgaaag ctgcccagcg cgagcagcag gaatgcccaa gacccgatgg
     1321 ccaggcaaac gtaaatccag cgtgtgaggt ttgagcgttc catgtgtcgt aatgcgtgat
     1381 gcgtggatgc gtaatgggta accggaccaa tcaatcttat ggttgcgcgt tacgagttac
     1441 gtgttacgtt tcagtgtcct gtatgaccaa acccgccctt gccgcgggcg gtctcatcga
     1501 gcgaaccgac ttccaccatc tccaccgccg gaacgggcag gatcagcatc tgcgcgatcc
     1561 gcatgccgcg ttcgatggtg aacggttggc ggccgtggtt gatcagcggc acgtgcactt
     1621 cgccgcggta atcgctgtcg atggtgccgg gggcgttgat cattgtgacg ccgtgcttgc
     1681 tcgacaatcc gctgcgcggg cggac
//
LOCUS       DATIMH010000512         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4745963, whole genome shotgun sequence.
ACCESSION   DATIMH010000512 DATIMH010000000
VERSION     DATIMH010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4745963"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..1817
                     /locus_tag="VLI90_08945"
     CDS             345..1817
                     /locus_tag="VLI90_08945"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HSV14373.1"
                     /translation="MLLLHDLLRQIHPPISLSSLPNVEVTGVQEDSRLVQPGNLFIAR
                     GGTKTDGAKFVEDAKARGAIAVVTATAVESSLPQAIVADPASAASVLAHALYGHPTET
                     LRVLGVTGTNGKTTTAYLVRHVLNKAKQRCGMIGTVEIDDGRSTRESTMTTPGAIEVA
                     QLMATMRDKGCRACAMEVSSHSLHQGRVAGVHFAGAAFTNLTGDHLDYHKTMHDYAAA
                     KATLFETLPSTGVAVVNAEDKWTPRMVANCHGRIIRYGFSKAADYRARDIAITAAGTH
                     FILHTPDGRAEMDLQLIGKHNIENALAAAALLGEVFGLSVHQLAAGLKDAQGAPGRLQ
                     AVRSGQPFAVLVDYAHTDDALENVLTALRPLTRGRLRVLFGCGGDRDRTKRPRMARTA
                     ERLADVVYVTSDNPRTENPQSILDEICGGFSAEKRRSVRVELDRRAAIEQVLADAEAN
                     DVVLIAGKGHENYQIIGTTKHHFDDVEECTRVLTQRGVAA"
     gene            2014..>2978
                     /gene="murF"
                     /locus_tag="VLI90_08950"
     CDS             2014..>2978
                     /gene="murF"
                     /locus_tag="VLI90_08950"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HSV14374.1"
                     /translation="MKPLSVQEIRQAVGGRLLTSQPTGLPVLCVCSDTRQMERSSLFI
                     AIKGDNHDGHQHLPAAAAGGAVAALVSDIPDNAPPNITLIQVADTRAAMGKLATYVRQ
                     QMSSKVIGVAGSNGKTSTKHLIHAALSGKFNGSISPKSFNNDIGVPLTIFPADPNQDY
                     LVLEMGTNHHGEIMKLTTMAEPDIAVITNVGAEHLEFLGDLMGVRRENATVVAGLNPK
                     GLLVVNGDDAELLDAVSTYPGKRVTFGFKETNDLWPADIQCSEQGIRYRLNGSRQEVF
                     VPMLGRHTACNSLAAIAVARRMGVSDKLIYESLAEANGPEWRLQL"
BASE COUNT          589 a          933 c          940 g          516 t
ORIGIN      
        1 gttcaccgcc gaggcgccga ggacgcagag gcagacgccg agaggaaatg gtaagaggtt
       61 gtaaccacag atttcacaga ttacgcggat tcagaaaagc attcccttcg atctgtgtaa
      121 tctgtgaaat ctgtggttac cgctcttcct cccctcggca tctgcctcgg cgctctcggc
      181 gcctcggcgg tgaatcgatg gccggaactc gacaatcgca catcctgctc gctaaccgcg
      241 ttgattcgac ccgttgcgtg tgatacgttt gctccgccgc gtgacatggt ggtcgcgcgg
      301 tatctgatcg cgctccactt ggcacttggg aaaaggacgt cccgatgctg ctccttcatg
      361 acctgctgcg gcaaatccat ccgccgatct cgctgagctc gctgcccaac gtcgaggtga
      421 ccggcgttca ggaagactca cgcctggttc aacccggcaa cctgttcatc gcccgcggcg
      481 gcacgaagac cgacggggcg aagttcgtcg aagacgccaa agcgcgcggg gcgatcgctg
      541 ttgtcacggc caccgccgtc gagtcatccc tgccgcaggc gattgtcgcc gatccggctt
      601 ccgccgcgtc ggtgctcgct catgcgctct atgggcatcc gaccgagacg ctgcgcgtgc
      661 tcggcgtcac cggcaccaac ggcaagacga ccacggctta cctcgttcgc cacgtgctga
      721 ataaggccaa gcagcgctgc ggcatgattg gcaccgtcga gatcgacgac ggccgatcga
      781 cgcgcgaatc gaccatgacg acgcctggag cgatcgaagt cgcccagttg atggcgacga
      841 tgcgcgacaa gggctgccgg gcctgcgcga tggaagtcag cagccattcg ctgcaccaag
      901 gcagagtggc cggcgtgcat ttcgccgggg cggcattcac caacctgacc ggcgatcacc
      961 tggactatca caagacgatg cacgactacg ccgccgccaa ggcgacgttg ttcgagacgc
     1021 tgccgagcac gggcgtggcc gtcgtcaacg ccgaggacaa gtggaccccg cggatggtcg
     1081 ccaactgtca tgggcggatc atccgttacg gtttcagcaa ggccgcggat tatcgggccc
     1141 gcgacatcgc gatcacggcg gcgggcacgc acttcatcct gcacacgccc gacggtcgcg
     1201 cggagatgga tctgcagctg atcggcaaac acaacatcga aaacgccctg gcggccgcgg
     1261 cgctgctggg cgaggtcttc ggtctatcgg ttcatcagtt ggcggccggg ctcaaagatg
     1321 cgcaaggagc gcccggtcgc ctgcaagcgg tgcgttccgg gcagccattc gcggtgttgg
     1381 tggactacgc gcacacggac gatgcgttgg aaaacgtcct gaccgcgctg cgtccgctga
     1441 cacgcggccg tcttcgcgtc ctgttcggat gcggcggtga ccgcgaccgc acgaagcgtc
     1501 cgcgaatggc gcgcaccgcg gaacgattgg ccgatgtcgt ctacgtcacc agcgacaatc
     1561 caaggaccga gaatcctcaa agtattttgg acgaaatctg cggcggcttc agtgccgaaa
     1621 agaggagatc ggtgcgcgtg gaactcgatc gtcgagccgc catcgaacag gtgctggccg
     1681 acgccgaggc gaacgacgtg gtgctgatcg ccggcaaagg gcacgagaac taccagatta
     1741 tcggaacgac gaagcatcac tttgatgacg tggaagagtg cacgcgggtg ctgacgcaac
     1801 ggggcgtcgc ggcgtgaacg agcgaaacca gacgtagagg tcgaacagcc catttagccc
     1861 ggcgtgaata cacgccgggt ttcgttgaga agtcgaggcc gttttgtttt ctcaatcgaa
     1921 ggatctcgaa gacccacata aacccggcgt gtgttcacgc cgggctaaat ggggttgttg
     1981 cagaatcgag cgagtgcgag cgcgcgcgat gagatgaaac cgctatcggt ccaggaaatc
     2041 cgccaggccg tgggcggccg gttgctgact tctcagccga ccggcctgcc ggtgctctgc
     2101 gtttgttccg acacgcggca gatggagcgt tcgtcgctgt tcatcgctat caaaggggac
     2161 aatcacgatg gccaccaaca cctgcccgcc gccgccgcgg gtggggctgt tgcggcgctg
     2221 gtttccgaca ttcccgacaa cgcgccgccg aacatcacat tgatccaagt cgccgacacg
     2281 cgcgctgcga tgggcaagct ggcgacgtac gtccgccagc agatgagttc caaggtcatc
     2341 ggcgtcgccg ggtccaacgg caagaccagc acgaaacatc tcattcacgc ggcgctgtcg
     2401 gggaagttca acggctcaat ctcgcccaag agtttcaaca acgacatcgg cgtgccgctg
     2461 acgatttttc ccgccgatcc gaaccaggat tacctcgtgc tggagatggg cacgaaccat
     2521 cacggcgaga tcatgaagct gacgacgatg gccgagccgg acatcgcggt catcaccaac
     2581 gtcggcgccg agcacctgga atttctcggc gacctgatgg gcgtgcgccg cgagaacgcg
     2641 accgtcgtcg ccgggttgaa ccccaagggc ctgctcgtcg tcaacggcga cgatgccgag
     2701 ctgctcgacg ccgtgtcgac ttatccgggc aagcgcgtga cgttcggctt caaggaaacc
     2761 aacgaccttt ggccggccga catccagtgc agcgagcagg gcattcgcta tcgcctgaac
     2821 ggcagccggc aggaagtctt cgtgccgatg ctcggccgcc acaccgcgtg caattccctg
     2881 gccgccatcg ccgtcgcccg ccgcatgggc gtgagcgata agctgatcta cgaaagtctc
     2941 gccgaagcga acggtcctga atggcgattg cagttgca
//
LOCUS       DATIMH010000513         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4453133, whole genome shotgun sequence.
ACCESSION   DATIMH010000513 DATIMH010000000
VERSION     DATIMH010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4453133"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VLI90_08955"
     CDS             complement(<1..247)
                     /locus_tag="VLI90_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14375.1"
                     /translation="MAARVRKTAKNGQHTRPTKSAGFIEPKPVHHGLSNFTKAMKFLG
                     TLADFEKLRIVRYNSQNFDLDRMRTLLKKLGNPQDHFR"
     gene            577..2085
                     /gene="rimO"
                     /locus_tag="VLI90_08960"
     CDS             577..2085
                     /gene="rimO"
                     /locus_tag="VLI90_08960"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HSV14376.1"
                     /translation="MAKSAKRSTSKKSVKTPAIRSVAFVSLGCPKNLVDSERMLGLLA
                     EDGLAITADASAADAIVINTCGFLEASKDESLKEIREAIELKKSGKCQRVVVAGCLVQ
                     RHKTKLLADAPGIDRLVGVFDREHIVEAVQGRENPRQEHGHFLGKYHDLSQELSKAHG
                     LLSVGFEKETKTSRLPVFENDRARLRLTPRHYAYLRISEGCNQGCSFCTIPSIRGLMR
                     SKPIEQILLEARELAADGAVELNLIGQDTTSYGTDIGYAEGLSGLLRTLDKQLKDVHW
                     LRLMYAYPSCFTDEMIDTIAQSSRIVKYIDMPLQHINDEILTKMKRRVTRREIEMLLA
                     KLRKIPGMAIRTTFIAGSPGETDAQHQELVQFVKDFGFDMMGVFPYSQEPGTPMGRMT
                     DQLPDNVKQARVEELMLAQQEVAFVKAKSTIGEQIDVLIDRPAGRDEEDGYVARSQSQ
                     APDIDSVVHVNANGTLLHPGQIASVKVTDFQAYDLVAEVPRKRARSLKVVTA"
     gene            complement(2075..2617)
                     /locus_tag="VLI90_08965"
     CDS             complement(2075..2617)
                     /locus_tag="VLI90_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14377.1"
                     /translation="MTDEPLPVIALEYRQGDEQTPWFAVVRLLSILCIISNAVVAFSS
                     IYYSWYYLDLDHRAAYVQLGYSSSWLLCSSTILLLSGLTSVCLVIGGWGCIKRKERWR
                     RATVRAAWVKVLLIAVQVVMTVGFYASRASQMDTAQAVIQLVTREVSGSVLLTIATAV
                     ILARPEVKRAFAASAAPITQ"
     gene            complement(2607..3170)
                     /locus_tag="VLI90_08970"
     CDS             complement(2607..3170)
                     /locus_tag="VLI90_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14378.1"
                     /translation="MNSPTAVNPSEPKPVVPLSYFERGGAALPPAVRLLALAAMVDGA
                     ILAGVFFLQTGFVLWEALSPRSASTAYFTLSSWQWVLSLPGAIAGTVFCIGGFLAMKL
                     RPAARRTLVVGAMLVIICTIATWASSALFLSSRSSGLPPSFRVYQLANNVMYAVRHCV
                     LPAVVWLIFRQPAVREIFATCAADNDR"
     gene            complement(3206..>3503)
                     /locus_tag="VLI90_08975"
     CDS             complement(3206..>3503)
                     /locus_tag="VLI90_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14379.1"
                     /translation="KVDPPPDLALEVDVTNWSIKRLPICAALAVPEIWRHDGKKLFGL
                     HLATEGEYEPAETSLAFPFLRVAELNRFLEMWPTTEETAVLRAFRDWVRAECPQ"
BASE COUNT          798 a         1082 c          990 g          633 t
ORIGIN      
        1 agcggaaatg atcctgcggg ttacccagct tcttcagcaa ggtccgcatg cgatctaagt
       61 cgaagttctg actgttgtaa cgaacgatgc ggagcttctc aaagtccgcc agcgtaccca
      121 aaaatttcat ggctttggta aaattgctca gaccatgatg aaccggcttg ggttcaataa
      181 agccagccga tttggtcggg cgggtatgtt ggccattctt ggccgttttg cgcactcgcg
      241 cggccatgac ggcggtccgt cgccgggggg tcgtgcgcct cgtcttcgat tgggtcgcca
      301 tggcgtcaaa tcatatcagg atcgtaggcc gctttcaaat atgtcaccca aatcgcgaca
      361 ataacgcaat ttgcgagtta gcagacagta gcgtgagaaa ttctcatatt aatattttgt
      421 tagggtcacg atcttgcgga gcaacggaag tcgtgactat ttgcgcggca ccatattcca
      481 aaccattata ggacctgaag cgtaccgctc tttcgcccgg cagcggacta ggataccctt
      541 cgccgccttg aatcgcagtt tgatcggagt ttgaacatgg ccaaatcggc caagcgctcg
      601 acatctaaga agtctgttaa gacgcccgcc atccgctcgg tggcgttcgt cagcctgggt
      661 tgtcctaaga acctggtgga cagtgagcgc atgctcggac ttttggcgga agatggcttg
      721 gccatcaccg ccgacgcgtc ggcggccgac gcgatcgtca tcaacacctg cggcttcctc
      781 gaagccagca aagatgaatc gctgaaagaa atccgcgaag cgattgaact gaagaagagc
      841 ggcaagtgcc aacgcgtcgt cgtcgcaggc tgtttggtgc agcgtcacaa gacaaagctg
      901 ctggccgatg ccccgggcat cgatcgcctg gtcggcgtat tcgaccgcga acacatcgtc
      961 gaagcggttc aaggaagaga aaacccacgc caggagcacg ggcattttct tgggaagtat
     1021 cacgatctgt cgcaggaact ttccaaagcc catggattgc tatccgtggg ctttgaaaaa
     1081 gagacgaaga cctcgcgcct gcccgtcttc gaaaacgacc gggcgcgcct gcgcctcacc
     1141 ccgcggcatt acgcgtacct ccgcatcagc gaagggtgca accaaggttg ctccttctgc
     1201 accatcccat cgatccgcgg attgatgcgc tccaagccga tcgaacagat cctgctcgaa
     1261 gcgcgcgagc tggccgccga cggcgcggtc gagctgaacc tgatcggcca ggacaccacc
     1321 agctacggca ccgacatcgg ctacgccgag ggtttgtcgg gtttgctacg aacgctcgac
     1381 aagcagctca aggacgtaca ttggctccgc ctgatgtacg cgtatccgtc ctgcttcacg
     1441 gacgagatga tcgacacgat cgcgcagtcc agccggatcg tgaagtacat cgacatgccg
     1501 ctgcagcaca tcaacgacga gatcctgacg aaaatgaaac ggcgggtgac gcgccgcgaa
     1561 atcgaaatgc tgctggccaa gctgcgcaag atccccggca tggcgatccg cacgacgttc
     1621 atcgcgggtt cgcctggcga aactgacgcg cagcatcaag agctcgtcca gttcgtcaaa
     1681 gacttcggct tcgacatgat gggcgtcttc ccgtacagcc aggaacccgg caccccgatg
     1741 ggccggatga ccgaccagct gccggacaac gtgaagcagg cgcgagtcga agagctgatg
     1801 ctcgcgcagc aggaagtcgc gttcgttaaa gccaagtcca caatcggcga gcaaattgat
     1861 gttctgatcg accgccccgc cggaagagat gaagaagacg gctacgtcgc ccgctcgcag
     1921 tcccaggccc ccgacataga cagcgtcgtc cacgtcaacg ccaacggcac gctattgcat
     1981 cccgggcaaa tcgcgagcgt gaaggtgacg gacttccagg cgtacgacct ggtcgcggaa
     2041 gtgccgcgca aacgggcgcg gagcctgaag gtcgttactg cgtgatcggc gccgcgctgg
     2101 ccgcaaacgc tcgcttcact tccggccgcg ccaagatgac tgccgtcgcg atcgtcaaca
     2161 gcacactgcc tgacacttcg cgagtcacca actgaataac cgcttgcgcc gtgtccatct
     2221 ggctcgcgcg cgaagcatag aaaccgactg tcatcacgac ttgtaccgcg atgagcagga
     2281 ctttcaccca cgcggcacgt accgtggccc gccgccaccg ttctttgcgc ttgatacagc
     2341 cccatccgcc tatcaccagg cagacgctcg tcaacccaga cagcagcaga atcgtcgacg
     2401 aacatagcag ccagctgctg ctgtagccca gttgcacata cgccgcgcgg tgatccagat
     2461 caaggtaata ccatgagtaa tagatggatg agaacgcgac gactgcgttt gagataatgc
     2521 agaggatcga tagcagtcgc acgaccgcaa accacggcgt ttgctcgtcg ccttggcgat
     2581 actccagcgc gatgacaggc agcggttcat cggtcattgt cggccgcaca ggttgcaaag
     2641 atctcccgca ccgccggctg ccgaaagatc aaccacacca ccgccggcag cacgcagtgc
     2701 cgcacggcat acatcacgtt gttcgccagc tggtagacgc gaaagcttgg cggtagaccg
     2761 cttgagcgag acgacaggaa cagagccgat gacgcccacg tcgcgatggt gcaaatgatg
     2821 accaacatgg ccccgacgac cagcgtccgc cgagcggccg gtcgcaactt catcgctaag
     2881 aatccaccga tgcaaaagac ggtgccggcg atcgcgcccg gcagcgagag tacccattgc
     2941 caactcgaca acgtaaaata tgccgtcgac gctgatcgcg ggctcagcgc ctcccaaagc
     3001 acaaaaccgg tctgaagaaa aaacacgccg gcgaggatcg cgccatccac catcgccgcc
     3061 agcgccagca ggcgaacggc aggcggcagc gcagcgccgc cgcgctcgaa ataagacagc
     3121 ggcacgactg gttttggctc gcttggattg accgccgtcg gggaattcat cacgcttgtc
     3181 ctggcgccac cgacgatcgg ctatttcatt gtggacactc agcacgcacc caatcgcgaa
     3241 acgctcgcag caccgccgtc tcttcagttg tcggccacat ctccagaaat cggttcagct
     3301 cggcaacgcg aaggaacgga aatgccaggc tcgtctccgc cggctcatat tcaccttcgg
     3361 ttgccaggtg caagccgaag agcttcttcc cgtcgtgtcg ccagatttcc ggcacagcaa
     3421 gggccgcgca aatcggcaga cgtttgatcg accagtttgt gacgtccacc tccagcgcca
     3481 aatcaggtgg tggatcaacc ttc
//
LOCUS       DATIMH010000514         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_989374, whole genome shotgun sequence.
ACCESSION   DATIMH010000514 DATIMH010000000
VERSION     DATIMH010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989374"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VLI90_08980"
     CDS             <1..1213
                     /locus_tag="VLI90_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSV14380.1"
                     /translation="QHRQAYDAGVHHKFHDQAIADLRAQGKPIDADSVENDEMHLALA
                     NVQPSVQSTLDEILEPLPKSSEVNWTPWFWLYGALAALPFVLIFATFKIYRRSHSYTG
                     RELAAGLMFASPWIIGFIVFVGGPIFFSILFAFTRYDVISPAHWVGLDNFHRIGTDAL
                     FSKSLGNTAFMIIRIPLTMVVSLLIAMLLNHALRGMGFYRTACYMPAIVPLIASSLLW
                     MWIFNPSQGPLNQGISWILETRPIEWLQNVTHHHISPPLWLQDENWSKPSLILMNIWG
                     AGAGIIIWLAGLQSVPTQLYEASAIDGAGPWRRFWNVTVPMLSPYILFNFIIGVIGTM
                     QIFNEAFVMTQGGPANSTLFYAYHLFQQAFQYFRMGYASSLAWILFVIVLVLTMIQLW
                     LSKRWVHYERT"
     gene            1200..3020
                     /locus_tag="VLI90_08985"
     CDS             1200..3020
                     /locus_tag="VLI90_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSV14381.1"
                     /translation="MSVLEDGLTTTAARAAADEAIPPVAPLPRLAPQPPRQNEYRRRS
                     RFAVYFTNHGWVHVLLLLSVWLFLFPFIWMLGTSMKTDEELMDAKLLPTIETFRPTSP
                     YVRPPEEPRKPTDVPTAQWKQLLPKLTAIASGAIARYQAVNPAEPTVATLDADAHRES
                     AAANLVTTAVGKIDRRLWTGAEKTLLDEYRSFLTEPAVAAALSDSLARLELSTFQLRT
                     LDLHSHMLSDGHFASTWKVESGDAKLFDTGDSTRLSYHFDSPSSPPVVLRYDFDLPDG
                     VKPQDLHKLTLSIKPDNSWHRVSATLDIAGNRWQADEVNYIAQHRPTAFLFQPPTFED
                     ELMRAKIWTSLKKIGPAPAGLAASAVSFRLIIEPSSTLQANWGKLWRNYDRAFKSVPF
                     WKYMTNSMLLVILCTTGSLFSATFVAYAFARLHWPGRSIAFLLVLSTMMLPGQVTMIP
                     GFMIWRALHWYNTLNPLWVPAFFGSAFFIFLMTQHMRTIPKDLEEAARIDGLNALQSW
                     YYIILPNVTPTLAAIAILSFMGSWNEFMGPLIYLRDQTKFPLSLGLYGLGLMRTTPGS
                     DFGWSVVMAGNMLMTVPVIVVFFLFQRYFVQGVTLTGMKG"
BASE COUNT          612 a         1164 c          918 g          665 t
ORIGIN      
        1 ccagcaccgc caggcgtacg acgccggcgt gcatcacaag ttccacgatc aggcgatcgc
       61 cgacttacgc gcgcagggaa aaccgatcga cgccgactcc gttgagaatg acgagatgca
      121 cctggcgctg gccaacgtcc agccctcggt gcagtcgacg ctggatgaaa ttctcgaacc
      181 gctgcccaag tcgtccgaag tgaactggac gccgtggttc tggctgtacg gcgcgctcgc
      241 cgcgttaccg ttcgtcctga tcttcgcgac gttcaagatc tatcgccgct cgcattcata
      301 caccggtcgg gaactggcag ccggtttgat gttcgcctcg ccgtggatca tcggtttcat
      361 cgtcttcgtc ggcggtccga tctttttctc gatcctcttc gccttcacgc gctacgacgt
      421 gatctcgccc gcacactggg tgggcctgga caacttccat cgcatcggga ccgatgcgct
      481 gttcagcaaa tcgctcggca acaccgcgtt catgatcatc cgcatcccgc tgacgatggt
      541 agtgtcgctg ctgatcgcga tgctgctcaa ccacgcgctg cgcggcatgg gcttctatcg
      601 caccgcgtgc tatatgcccg ccatcgtgcc gctgatcgcc tcgtcgctgc tgtggatgtg
      661 gatcttcaac cccagccagg gcccgctcaa tcaaggcatc agttggatcc tggaaacgcg
      721 gccgatcgaa tggctgcaga acgtgacgca ccaccacatc tcgccgcccc tttggctgca
      781 ggatgaaaac tggtcgaagc cgtcgctgat cctcatgaac atctggggcg ccggcgccgg
      841 catcatcatc tggctggcgg gccttcaatc ggttccgacg cagctgtacg aagcctccgc
      901 catcgacggc gccggcccgt ggcgcaggtt ctggaacgtc accgtgccga tgctctcgcc
      961 gtacattctg ttcaacttca tcatcggcgt gatcggcacg atgcagatct tcaacgaagc
     1021 cttcgtgatg acccagggcg gcccggcaaa ctccacgctg ttctatgcct atcacctgtt
     1081 ccagcaggcg ttccaatatt tccgcatggg ttacgcgtca tcgctggcgt ggatcctgtt
     1141 cgtgatcgtg ctcgtgctga cgatgattca gctctggctg agcaagcggt gggtccatta
     1201 tgagcgtact tgaagacggc ctgacaacca ccgctgcgcg cgcggccgcc gacgaggcga
     1261 tcccgcccgt cgcgccgttg ccgcgcctcg cgccacagcc gccgcgccaa aacgaatacc
     1321 gccgccgcag ccgcttcgcc gtctacttca ccaaccacgg ctgggtgcac gtgctgctgc
     1381 tgctgagcgt ctggctgttt ctcttcccgt tcatctggat gctcggcacg agcatgaaga
     1441 cggacgaaga gttgatggac gccaagctgc tgccgaccat cgaaaccttc cgcccgacca
     1501 gcccgtacgt tcgtccaccg gaagaaccgc gcaaaccgac cgatgtgccg accgcgcagt
     1561 ggaagcagct gctgccaaag ttgaccgcca tcgcatcggg cgcgatcgcg cgctatcaag
     1621 cggtgaaccc cgccgaaccc acggtcgcca cgctcgatgc cgacgcccac cgcgaatccg
     1681 ccgccgccaa cctggtcacc accgccgtcg gcaagatcga ccgccgcctg tggaccggcg
     1741 ccgagaagac gctgctggat gaataccgct cgttcctgac cgagcctgcc gtcgccgccg
     1801 cgctgtcgga ttcgctggcc cggctcgagc tgtcgacgtt ccagctccgc acgctggacc
     1861 tgcatagcca catgctgagc gacggccact tcgcgtcgac ttggaaggtc gaaagcggcg
     1921 atgccaaact cttcgacacc ggcgactcga cgcggctctc gtatcacttc gactcgccgt
     1981 catcgccgcc cgtcgtcctg cgatacgatt tcgatctacc ggatggcgtg aagccgcaag
     2041 atctgcacaa gctgacgctg tcgatcaagc ccgacaactc ctggcaccgc gtcagcgcca
     2101 cgctcgacat cgctggcaac cgctggcaag cggatgaagt caactatatc gcgcagcaca
     2161 ggcccaccgc gttcctcttc caacccccaa cgttcgaaga cgaattgatg cgcgccaaga
     2221 tctggaccag cctgaaaaag atcggccccg ctccggccgg acttgccgct tcagcggtaa
     2281 gtttccgcct catcatcgag ccctcctcaa ccctccaggc gaactgggga aaactctggc
     2341 gcaactacga tcgcgccttc aagtccgttc ccttctggaa gtacatgacc aactcgatgc
     2401 tgctggtcat cctctgcacc accggctcgc tcttcagcgc caccttcgtc gcctacgcct
     2461 tcgcccgcct gcactggccc ggccgatcga tcgccttcct cctcgtgctc tccaccatga
     2521 tgcttcccgg gcaggtcacg atgatccccg gattcatgat ctggcgcgcc ctgcactggt
     2581 acaacacgct gaaccccctc tgggtccccg cgttcttcgg ttccgcgttt ttcatcttcc
     2641 tcatgaccca acacatgcgg acgattccca aggacctgga agaagccgcg cgcatcgacg
     2701 ggctcaacgc gctgcagagt tggtattaca tcatcctgcc caacgtcacg ccgacgctgg
     2761 ccgctatcgc catcctgtcg ttcatgggct cgtggaacga gttcatgggc ccgctgattt
     2821 acctgcgcga ccagacgaaa ttcccgctat cgctcggcct gtacggcctg ggcctcatgc
     2881 gcaccacccc cggcagcgac ttcggttggt ccgtcgtcat ggccggcaac atgctcatga
     2941 ccgtgccggt catcgtcgtc ttcttcctgt tccaacgcta cttcgtccaa ggcgtgacgc
     3001 tgacgggaat gaaagggtga gtcaaagaaa tgcaaccgcc gagggcgccg agcacgccga
     3061 ggcggacgcc aagacataag agaagagttt ttaaccacag atttcacagc ctgacacaga
     3121 ttgaggacgg gctgttccca atctgtgcaa tctgtgaaat ctgtggttaa aaacaccctc
     3181 tctcctctcg gcgtccgcct cggcgtgctc ggcgccctcg gcggttgcct tggctcagtc
     3241 cagcagatgc gttcgatctt ctcgcgatgc cgatcggtgt ttcaatatca tcaagaaaca
     3301 gcacaggccg ctggcccgtg tcatgcgtgc gctgcgcggc tcccggcaac atgacacgg
//
LOCUS       DATIMH010000515         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1858881, whole genome shotgun sequence.
ACCESSION   DATIMH010000515 DATIMH010000000
VERSION     DATIMH010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858881"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VLI90_08990"
     CDS             <1..1173
                     /locus_tag="VLI90_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14382.1"
                     /translation="AIELKPDLAEAHNVLGVLWKARRRPADALAEFVRATHLRPHYAE
                     ACNNAGSVLELLGRPAEATRFYENAIVIQPDTAQFHENLALNLLQLGNYPAGWAEHEW
                     RRREPTNPASRIFPVPEWDGSPLNGRAIFLHAEQGVGDTVHFARYIPLVREHAGAGRI
                     IVEVQPSLANLIRHGIRGVDEVVPQGQVPASPMDLHKPLLSLPPLFGTTVQTIPNQPY
                     LTPDPAKVAAWAAKLPPRDRTLRIGLTWSGNPRHKNDRNRSCPPAQLEPLGSVEGVSY
                     ISLRKGAAVQNPPASLRLIDLTEQLQDFADTAALMANLDLLVSVDTAVVHLAGAMGMP
                     GWVMLPYVADWRWLLGREDSPWYPSLKLFRQATAGDWTNVVERVRAALSEYVAGRR"
     gene            complement(1420..1656)
                     /locus_tag="VLI90_08995"
     CDS             complement(1420..1656)
                     /locus_tag="VLI90_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14383.1"
                     /translation="MRPPMIGNWQVRVEAVRPFTIHGDEYYELQVTRIDTPDEAAGLV
                     LRVPEHATAGEPVAGQRLEITFLMGQVTAARIMQ"
     gene            complement(1682..1984)
                     /locus_tag="VLI90_09000"
     CDS             complement(1682..1984)
                     /locus_tag="VLI90_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14384.1"
                     /translation="MESILPDSDTAALRDALSSGAPDAAVRERNGSTYALRNLLSTFR
                     AVRALAERRRHRPLPRLNATRRAHRETRRRLAHTAADPARIRRSHPLPEDLQWFVA"
     gene            complement(2041..2370)
                     /locus_tag="VLI90_09005"
     CDS             complement(2041..2370)
                     /locus_tag="VLI90_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14385.1"
                     /translation="MESAIRNELKPGTPVRVVQQIAARNYTWTSDIRGVVVEYRQQQT
                     GSWFAHSKDDKLWLDRLTLRKIDGELITLNLDGFSHVEVDQTAAAAGAAPVAGDGEKK
                     NATDLPV"
     gene            complement(2555..>3467)
                     /locus_tag="VLI90_09010"
     CDS             complement(2555..>3467)
                     /locus_tag="VLI90_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HSV14386.1"
                     /translation="NAPVALLYDPASFDGGLTLNANAAVSFAASIAQAGVTPRWIAGL
                     NIADNASVMLAPKSGASDATVLVLSQLNVAPTGRLDLGYGALIAKGGNLATITAKLAT
                     GMSTAGLGAWNGYGIMSATAAGDSSHIRAVGVMLNDNGHGGPIYGATTPYGPFEGQNP
                     ATGDVLVRLTYYGDADLSGQVDGGDYSKTDNGFNTRMGTSAGWSNGDYNYDGTVDGSD
                     YALIDNAFALQSAQPAARPVGYLPPPMTASTASVVDAHPAAQIRAASAPSSPALKVFL
                     VRDLLSAPDDLHGVPNKSPDGPTLLQK"
BASE COUNT          577 a         1099 c         1179 g          612 t
ORIGIN      
        1 gcgatcgaac tcaagcccga tctagccgag gcgcacaacg tcctgggcgt tctgtggaag
       61 gcccgccgtc gcccggccga tgcactggcg gagttcgtcc gcgccactca ccttcgcccg
      121 cactatgccg aggcctgcaa caacgccggc tccgtcctcg agctgctcgg ccgtcccgcg
      181 gaggcgacgc gtttctatga aaacgccatc gtcatccagc cggacacggc ccagtttcac
      241 gagaacctgg cgctgaacct gctgcagctc ggcaactacc ccgcgggctg ggccgagcac
      301 gaatggcggc gccgtgaacc gaccaacccc gcgagccgga tctttcccgt gccggaatgg
      361 gacggctcac cgctcaacgg ccgcgcgatc ttcctccacg ccgagcaagg cgtcggcgac
      421 accgtgcact ttgcgcgcta catcccgctg gtccgcgagc acgccggcgc ggggcggatc
      481 atcgtcgagg ttcaaccgtc gctggcgaac ctgatccgcc acgggatccg cggcgttgac
      541 gaagtcgtcc cgcaggggca agtcccggcg tcaccgatgg acctgcacaa accgctgctg
      601 tcgttgccgc cgctgttcgg cacgacggtg cagacgattc cgaatcagcc gtatctcacg
      661 cccgacccgg cgaaggtcgc cgcgtgggcc gccaagctcc cgccgcgcga ccggacgctg
      721 cgcatcgggc tgacgtggtc cggcaatccg cggcacaaga acgatcgcaa ccgatcgtgc
      781 ccgccggcgc agctcgagcc gctcggctcc gtcgaaggcg tgtcgtacat cagcctgcgc
      841 aaaggcgccg ccgtgcaaaa cccgccggcg tcgctgcggt tgatcgacct gaccgagcaa
      901 ctgcaagact tcgccgacac cgcggcgttg atggcgaacc tggacctgct cgtcagcgtg
      961 gacacggccg tcgttcacct ggccggcgca atgggaatgc cgggctgggt gatgctcccc
     1021 tacgtcgccg actggcgctg gcttttgggc cgcgaggatt cgccgtggta tccgtcgttg
     1081 aagctgttcc gccaggcgac cgcgggggat tggacgaacg tggttgagcg ggttcgcgcc
     1141 gcgctgagcg agtacgtcgc ggggcggcgg taatttctgg tcatcctgag tcgagcacgt
     1201 actcgtgcgc agtcgaagga tctggccaag tagtcggtga actttcactc gcgcaacgac
     1261 agccgcttgc ggcttagcgt cttttgtatt caagaaaaga cgctaagcca caagcggctg
     1321 cgaccaacaa agtcgttcgc tgaattcccg aatagatcct tcgactccgg cgagtacgcc
     1381 tgcgctggag gatggcatgc gcgcgtatag cgcggctcct tactgcatga tccgcgccgc
     1441 cgtgacttgg cccatcagga aagtgatctc cagccgctgc cccgcgaccg gttcgccggc
     1501 cgtggcgtgt tctggcacgc gaagaaccaa acccgccgcc tcatccggcg tgtcgatgcg
     1561 cgtcacctgc agttcgtaat actcatcgcc gtggatggtg aacggccgga ccgcttccac
     1621 gcgcacttgc cagttgccaa tcatcggggg tcgcatggcg atccagtcta cagcgcgagc
     1681 gtcaggcgac gaaccactga agatcctcgg gcagcgggtg gctgcgccgg atgcgtgcag
     1741 gatcagcggc cgtatgagca agacgccgcc gcgtttcacg gtgcgctcga cgggtggcgt
     1801 tgaggcgcgg cagcgggcga tgtcgtcggc gttcagcgag ggcgcgaaca gcgcggaagg
     1861 tgctgagcag attgcgcagc gcgtatgtcg atccattgcg ctcgcgcacg gcggcgtcgg
     1921 gcgctccgct gctcagcgca tcgcgcagag cggcggtatc agaatcggga agtatggatt
     1981 ccacgacggc aaagccgtcg ctcgcgatgg tctgctgaag cgactggggg aacgccgtgg
     2041 ttacaccggt aggtcggttg cgtttttctt ttcaccgtcg ccagccaccg gcgcggcacc
     2101 ggcagcggcg gcagtttgat cgacctcgac gtggctgaac ccatcgaggt tcagcgtgat
     2161 caattcgccg tcgatcttgc ggagcgtcag gcggtcgagc cagagcttgt cgtccttgga
     2221 atgggcgaac cagctgccgg tttgctgctg gcggtactcg acgacgacgc cgcggatgtc
     2281 gctggtccac gtgtaattgc gggcggcgat ctgctggacc acgcgaacag gcgtgcccgg
     2341 tttcagttca ttgcgaatgg cggactccat agaatgcaag aacgtaaggg cgcgggcgcg
     2401 aaaggtaaag gggtgcaacg agatcaatga cgcgcgcgac gcgagcgaag cgccggatgt
     2461 acggcctgac gaaccgacac tgttgcagga atggttcaga atcgaccacg ccgggttcgg
     2521 agtacctccg aacccggcgt gggtgggtcc cttatcactt ctgcaaaagt gtcgggccgt
     2581 cgggactttt atttgggact ccatgtagat cgtcgggcgc agatagcagg tcacgcacga
     2641 ggaacacttt cagcgctgga cttgatggag cagacgcggc ccggatctgt gccgccgggt
     2701 gcgcgtcaac cacgctcgcg gtcgaggcgg tcatgggtgg cggtagataa ccgaccggtc
     2761 gagcggcggg ttgggcagat tgcagcgcga acgcgttgtc gatcagcgcg tagtcgctgc
     2821 catcgacggt gccgtcgtag ttgtagtcgc cgttgctcca gccggcgctc gtgcccatcc
     2881 gcgtgttgaa gccgttgtcg gttttgctgt agtcgccgcc gtccacttgc ccgctcaagt
     2941 cggcgtcgcc gtagtaggtg aggcggacca gcacatcgcc ggtggcgggg ttttggcctt
     3001 cgaacgggcc atagggtgtc gtcgcgccgt agatcgggcc gccgtggccg ttgtcgttca
     3061 gcatgacgcc gacggcgcgg atgtgcgatg aatcgccggc tgcggtggcg ctcatgatgc
     3121 cgtaaccgtt ccacgcgccc aggcccgcgg tgctcatgcc ggtggcgagc ttggcggtga
     3181 tcgtcgccag attgccgcct ttggcgatca gcgcgccgta gccgagatcc agtcgcccgg
     3241 tgggcgcgac gtttagttgc gacaacacca gcaccgtcgc atcgctggcg ccggatttcg
     3301 gcgcgagcat tacgctggcg ttatcggcga tgttcaggcc ggcgatccac cgcggggtga
     3361 cgccggcttg cgcgatcgac gccgcgaacg acacggcggc gttcgcgttg agggtcaagc
     3421 cgccgtcgaa ggaagcgggg tcatacagca gcgcgaccgg cgcgttg
//
LOCUS       DATIMH010000516         8410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2721206, whole genome shotgun sequence.
ACCESSION   DATIMH010000516 DATIMH010000000
VERSION     DATIMH010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721206"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="VLI90_09015"
     CDS             complement(<1..1468)
                     /locus_tag="VLI90_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14387.1"
                     /translation="MSIGQSVLIAHASGSTVTATGTNGTAGAAGPAGQNGGDGTNGAD
                     ATATANVPSDTNNTATATGGTGGAGGIAGFASSPPFTEGGAGGNGGTAAATATNGNTP
                     VASNVTSSATATGGVGGAGGPSGSSNETPGTSGTGGAADAIANASSSSGTATTSVTAK
                     GGAGGAAPTNFFGGAGGAAGAGVAGASESTTDSSGGTIISTEQATGGIGGAATNGTGG
                     TGGAATVSNQADATGNSSVTLTFSATGGAGGADVSGAGGPGSGSGGNGGVANFGSVSA
                     SSTGGSVSITGNLFGGNGGSGQSSGPSNGGNGAVETADNAVTGSTSGNLSLIQNCTGG
                     SGGTGNSSNDTITPTAGAGGAANSSLSLSTACSSLSLTTKATGGAGGGVTAFGRETAA
                     DGAAGTASATGTNASGRTTINVTATGGTGGTGGRAQTNSLVSSNGGNGGVGVATGVGN
                     ASGNNAVSITAAATGGNGGSVTAGGSNVISNPGDTAGNG"
     gene            complement(1799..3625)
                     /gene="hrpA"
                     /locus_tag="VLI90_09020"
     CDS             complement(1799..3625)
                     /gene="hrpA"
                     /locus_tag="VLI90_09020"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HSV14388.1"
                     /translation="MIESTSPSQALRERIEQCMLRDRHPLRQRFRRAEQLARDGRPAS
                     RLIDELARDVDASSTRRTRRLHERPRPSFPLDLPVVQRREEIARAIAENQVVVLCGET
                     GSGKTTQLPKICLELGRGVDGMIGHTQPRRIAARSVASRIAQELGVALGNVVGYKVRF
                     GDRTNPDTYVKLMTDGILLAETQGDRLLEAYDTLIIDEAHERSLNIDFLLGYVKQLLP
                     KRPDLKVIITSATIDPQRFSRHFNDAPIIEVSGRTYPVELRYRPLKSEDPDEEDREQT
                     QAIIEAVDELARHGPGDVLVFLSGEREIRETAEELRKHHPAGTEILPLYARLSGDEQT
                     KIFQPHTKRRIVLATNVAETSLTVPGIKYVVDPGFARISRYSARTKVQRLPNDPVSRA
                     SADQRKGRCGRVSEGVCIRLYAQEDYEKRPLFTEPEILRTNLAAVILQMKALRLGNID
                     HFPFVEPPDARLIRDGYLTLHELGAVDEEDQLTPTGRQLARLPIDPRLGRMILAAHDE
                     NCLDEVLIITSALSVQDPRERPTELAQVADEAHKQFVDEESDFLSYLKMWAFFQEKSK
                     HLSRSQLRKQCKQQFLSYLRMREWQDVHSQLCEVVSRLSDHQ"
     gene            3695..4687
                     /locus_tag="VLI90_09025"
     CDS             3695..4687
                     /locus_tag="VLI90_09025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSV14389.1"
                     /translation="MPGPLGTGDTPVLRRALLRRGGRLQCSDMLRRNVLVFHTGALGD
                     FVLSWPLALALGRIYAQSRIIFITHAQKGALAERVLRLESTDIEAGWHALFTENATHL
                     PPRAAGMLSGAHAIYTFATPSPEWLANVARLNPHAKVVALQPRPPEKYAQHASQWLLD
                     QLCDYTVERAATEQLLRSIAARGVGFNRTGSARDIVIHPGSGSPAKCWSIEKYLELAE
                     RFRAAGHTVRFIVGEVEQERWPASALNALAAAGDLRRPETYLDLLNELATAGTFIGND
                     SGPGHLAAIIGVPTVSLFGPTDPRVWQPVGPSVQVVRAEPLSSLSVDQVLEVLI"
     gene            complement(4802..6460)
                     /locus_tag="VLI90_09030"
     CDS             complement(4802..6460)
                     /locus_tag="VLI90_09030"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HSV14390.1"
                     /translation="MDDAQSSSSAASPATAQDAPAAPPSPHPSSGGVKETIESILVAF
                     VLAFIFRAFVVEAFVIPTGSMAPTLMGAHMRFQCPDCGYKFDVNWSSPSAGDSDDIDI
                     RANAGRITLPLYCPNCGFQMDPSQVENPAVYYGDRILVLKYLYLFQQPTRWDVVVFKS
                     PVSPGRYNYGQNYIKRLIGKPGEAIMVLDGDVYVRAPNAKSANDFTVQTKPKHVQDAL
                     WRLVYDNDYRPQNNLDRGNVPSFHLPWTASDGTGWNVNDMSHGRVFGFDNNTGGGTLR
                     FEPNANPTAQTTSDFLIYDIAPPNTKHPSGVYPEPWEFPVSDLDLQLTYERTAGDGPL
                     SLTLTKRNHAFTAEITPTDATLFHIVNGERVKIGRPMPLDHSSRPVRVELSNADYQVT
                     LRIDDKIAAQTTPADYAPDLKQLLQEYRSRVKGPPPRVEIAADRQRCQLSHLSLWRDV
                     FYYNSSPGVRWATPDSFPENAQLLGPDEFFVMGDNSLVSYDARCWTEPIELPGEDLRV
                     DAGRVPARFMLGKAFFVYWPAGYRPLSNVPAAFVPNFGDMRMIH"
     gene            complement(6557..7771)
                     /locus_tag="VLI90_09035"
     CDS             complement(6557..7771)
                     /locus_tag="VLI90_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HSV14391.1"
                     /translation="MTNLKDARRASDSPPSTGKPKHVAIIVGLGFSPVTEMLRGIADY
                     VQEHDPWAIYLKQYGSEAPPNWFRDWQGDGIMVYSPRVRDEELRECRVPIVDLAGHLN
                     TKGIPLVCLHNEAIGRVGAEHLLDRGFKHFAFYDNIEFEWSRGRREAFVAALKARGLP
                     CAIYSTRLQINHIGAAQGWEKQQQELAQWLKDSLPKPAGVMASSDLMGQNLLEACLLA
                     GISVPEEIAVLGVDNDETICRIASPPMSSVMANHVARGYVAAEVLDHLMQGKPGPTEA
                     VLIEPAGIAPRASTDMMAIEDPDVVRALQVIRERAYTNVGINDIVGSIAVSRTVLQRR
                     FRKLVGRSIHEEIIRLRLNRAIELLHDTTLEIKAIARKSGFKSQAYMTAVFQQRLGRT
                     PGSYRTHVKSLA"
     gene            7958..>8410
                     /locus_tag="VLI90_09040"
     CDS             7958..>8410
                     /locus_tag="VLI90_09040"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HSV14392.1"
                     /translation="MTTKPMFDLAYEGGFAVGAFNVNNMELAQGIIEACAAEKSPLIL
                     QISKGARKYAKMHYLKAIIDAAVAEYPWLPIAIHLDHGDTVDLVKTCIADGFTSVMID
                     GSHHPYDENVKVTAEAVKVAHASGVTVEAELGMLGGIEEDVVGMDAHEY"
BASE COUNT         1404 a         2697 c         2658 g         1651 t
ORIGIN      
        1 cgccgttgcc ggcggtgtca cctgggttgg agatgacgtt gcttccacca gcagtgacgc
       61 tgccgccgtt gccgccggtt gccgctgctg tgattgaaac cgcattgttc ccgcttgcgt
      121 tgcccacgcc tgtcgcgact cccactcctc cgtttccccc gttgctggag accagcgagt
      181 tggtttgggc acgtcctccg gttccccccg tcccgccggt cgccgtgaca ttgatcgtcg
      241 tccgaccaga ggcgttggtg ccagtcgcgc ttgcggttcc cgctgcgccg tcggccgcgg
      301 tttccctgcc aaaggcggtc acaccgccgc cggctccgcc ggtcgctttg gtcgtgagcg
      361 acaatgacga gcaagccgta gagaggctta gcgagctgtt tgcagcgccg cccgcgccgg
      421 cggtgggggt gatcgtgtcg ttgctgctgt ttccggtgcc gccactgcct ccggtgcaat
      481 tctgaatgag gctgagattg cccgacgtac tgccggtcac ggcattgtcc gcggtctcga
      541 cggcgccgtt cccaccgttg ctcggtccag agctctggcc gctgccaccg ttgccgccga
      601 acaggttccc agtgatagaa acgctgccgc cggtggaact ggcgctgacg gagccgaagt
      661 tagcaacgcc cccgttgccc ccgctgccac tgccgggtcc accggcaccg ctcacatctg
      721 cgccgcccgc gccaccggtt gcactgaaag tcagcgtcac gctgctgttg ccggttgcgt
      781 ctgcttggtt ggataccgtt gcggctccgc cagttccacc cgttccattt gtggccgctc
      841 caccgatccc accggtagct tgttcggtag agatgatggt tccaccagaa gaatcagtag
      901 ttgactcaga tgcacctgca acaccggcgc cggcggcacc gccggcgccg ccgaagaagt
      961 tcgtcggagc tgcgccgccc gctccaccct ttgccgtcac agaagtggtg gctgtgccgc
     1021 tcgaactgga cgcgttggca atcgcatccg ccgcgccgcc cgtcccgctg gtgcccggcg
     1081 tttcgttgct gctgccagat ggcccgcccg ctccacccac gccgcccgtt gcggtcgcgg
     1141 aggaggtgac attgctggcc accggtgtgt tgccgttggt tgctgtggct gccgccgttc
     1201 cgccgttgcc acccgctcca ccttcggtga agggtggcga cgaagcaaaa ccggcgatgc
     1261 cgcccgcgcc accggtacca ccggtcgccg tcgccgtgtt gttcgtgtcg ctcggcacat
     1321 tggcggttgc cgtggcgtcc gcgccgttcg tgccatcgcc gccgttttgt ccagcaggcc
     1381 cagccgcgcc ggcagtccca ttagttccag tcgccgtaac cgttgatcca gaggcatggg
     1441 cgatcaacac cgattggcca atcgacatgg cgagccaaag cgcgccggca cgagcagcgc
     1501 gcgccgttcc acctttagat aaagttcgtc cttgatcccg cgtcgaacgt ttagacatca
     1561 tcgttcccct ctggacagcc caccctggat atgcgtcgcg gccagtacaa atggttcagc
     1621 gatcaaaaac tgcgcccgtt ttcgttggcc gatcttttac ccgcacatgc agaacgtctc
     1681 aacttcaacg ggtcgcatat ttacggatca atcgggctca tcggaaggtg cgccatgggc
     1741 gcgaacagca gcagcgcatc cgccattgcg cccccgccca tgatgtcgcc cggcaaactt
     1801 actggtgatc cgacagccgg gaaaccactt cgcacaactg gctgtgcacg tcctgccatt
     1861 cgcgcatgcg gaggtacgag aggaactgct gcttgcactg cttgcggagt tgcgatcttg
     1921 aaaggtgctt ggacttctcc tggaagaacg cccacatttt tagatagctg aggaaatcgc
     1981 tctcctcgtc cacgaactgc ttgtgggctt cgtccgccac ctgggccagt tcggtcggcc
     2041 gctcgcgcgg gtcttgcacc gacagcgctg atgtgatgat cagcacttca tcgaggcagt
     2101 tctcgtcgtg cgcggcgagg atcatccggc ctagccgcgg gtcgattggc aggcgcgcca
     2161 gctgccggcc agtgggtgta agttggtctt cttcatcgac cgcgcccagt tcgtgcagcg
     2221 tgagatagcc gtcgcgaatg aggcgggcat ctggcggttc gacgaagggg aaatggtcga
     2281 tgttccccag ccgcagggcc ttcatctgca ggatgaccgc ggccaggttg gtgcgcagga
     2341 tttccggctc ggtgaacagc ggccgcttct cgtaatcctc ctgcgcgtat aggcgaatgc
     2401 acacgccttc gctgacgcgg ccgcatcggc ccttccgctg gtcggcgctg gcgcgcgaaa
     2461 ccgggtcgtt cggcagccgc tgcaccttcg tgcgcgcgct gtaccggctg atgcgcgcga
     2521 agccgggatc gacgacgtac ttgatccccg gcaccgtcag cgatgtctcg gcgacgttcg
     2581 tcgccagcac gatccgccgc tttgtgtgcg gttgaaaaat cttcgtctgc tcgtcaccgg
     2641 ataaacgcgc atacagcggc aggatttccg tccccgcagg gtgatgcttg cgaagctctt
     2701 cagccgtctc gcgaatctcg cgctcgccgc tgaggaacac cagcacatcg cccggcccat
     2761 gccgcgccag ctcgtccacc gcttcgatga tcgcctgcgt ttgctcgcgg tcttcttcgt
     2821 ccgggtcttc agacttcagc ggccgatagc gcaactcgac cgggtacgtc cgccccgaca
     2881 cttcgatgat cggcgcatca ttgaaatgcc tgctgaaccg ctgcgggtca atggtggccg
     2941 acgtgatgat gactttcagg tccggccgtt tcggcagcaa ctgcttgacg taaccgagaa
     3001 ggaaatcaat gttcaggctg cgctcgtgcg cttcgtcgat gattagcgtg tcgtacgcct
     3061 ccagcaaccg atcgccctgc gtctcggcca gcaggatgcc gtcggtcatc agcttcacgt
     3121 acgtgtcggg gttcgtgcga tcgccgaagc gaaccttgta accgacgacg ttgcccagcg
     3181 ccacgcccag ttcctgcgcg atgcgggacg cgacgctgcg cgccgcgatt cgccgcggct
     3241 gcgtgtgacc gatcatccca tcgacgccgc ggcccaactc caggcagatc ttcggcagct
     3301 gggttgtctt gcccgaccca gtttcgccgc acagcacgac gacctgattc tccgcaatcg
     3361 cccgggcgat ctcctcgcgc cgctgcacga ccggcaggtc gagcgggaac gacggccgcg
     3421 gccgctcgtg caaccgccgc gtccgccggg ttgacgaagc gtcaacatcg cgagcaagct
     3481 cgtcgatcag cctgctcgcc ggccgcccgt cgcgcgccaa ctgctccgcc cgccgaaatc
     3541 gctggcgcag cggatggcgg tcgcgcagca tgcactgctc gatgcgctct cgaagcgcct
     3601 gcgacggcga tgtggattca atcatcgaca cggtttgaat tatacgacgc ccaagtggcg
     3661 gcgagcgcat tgtcgtagca cgggcgtctc gcccgtgcct ggcccgcttg gcacgggcga
     3721 cacgcccgtg ctgcgtcgcg cattgctccg ccgcggtggc cgattacagt gcagcgacat
     3781 gctgcgtcgc aacgtcttgg tcttccacac cggcgcgctg ggcgattttg tgctcagttg
     3841 gccattggcc ctggcgctgg ggcggatcta cgcgcagagc cggatcatct ttatcacgca
     3901 cgcgcagaag ggcgcgctgg ccgagcgcgt gctgcgactg gagtccaccg acatcgaggc
     3961 aggatggcac gcgctgttta cggagaacgc gacacatctc ccgccgcggg cggcggggat
     4021 gttgagcggc gcgcacgcga tctatacgtt tgccacgcct agccctgagt ggttggcgaa
     4081 tgtcgctcgc ttgaacccgc acgcgaaagt ggtggcgctg cagccacggc cgccggagaa
     4141 atacgcgcag cacgcgtcgc agtggctgct cgatcaacta tgcgattaca cggtcgagcg
     4201 agccgcgacg gagcagttgc tgcgttcgat cgccgcccgc ggcgtggggt tcaatcgaac
     4261 cggctcggcc cgcgacatcg tcatccatcc cggcagcggt tcgccggcga aatgttggtc
     4321 gatcgaaaaa tacctcgagc tggcggaacg atttcgcgcc gccggtcaca cggtgaggtt
     4381 catcgtcggt gaagtcgagc aagagcgttg gccggcaagc gcgctgaacg cgctggcggc
     4441 ggcgggggac ctccgccggc cggagactta cctcgatctg ctgaacgagc tcgccaccgc
     4501 cggcacgttc atcggcaacg acagcggccc cggccatctg gccgcgatca tcggggtgcc
     4561 gacggtgtcg ctgttcggac cgactgaccc gcgcgtgtgg cagccagtgg gtccgagtgt
     4621 gcaggtcgtg cgcgccgagc cgctttcatc attgtcggtg gatcaagttt tggaagtgtt
     4681 gatctgactc gccacgccgg gttcggaggt actccgacga agcgagattc tgcggcgccg
     4741 gaagtgacga atccgagtcg tcggagtacc tccgaacccg gcgtaggggc gagcgaaact
     4801 attagtgaat catccgcata tccccaaagt tcggcacgaa cgccgcgggg acgttggata
     4861 gcgggcgata cccggccggc caatagacga agaacgcttt gccgagcatg aaccgcgcgg
     4921 gcactcggcc cgcgtccacg cgcaggtctt cgcccggcaa ttcgatcggc tccgtccagc
     4981 agcgcgcgtc atagctcacc agtgaattgt cgcccatcac gaagaactcg tcggggccca
     5041 gcagttgcgc gttttccgga aagctgtctg gcgtcgccca gcgcacgccg gggctgctgt
     5101 tgtaatagaa cacgtcgcgc cacaggctca ggtgtgacag ctggcagcgc tgccgatcgg
     5161 cggcgatctc cacccgcggc ggtgggccct tcacgcgcga acgatattcc tgcagcagct
     5221 gcttcaggtc cggcgcgtaa tccgccggcg tcgtctgcgc ggcaatcttg tcgtcaatcc
     5281 gcagcgtgac ctggtaatcc gcgttgctca actcgaccct caccggtcgc gacgaatgat
     5341 ccagcggcat cggccggccg atcttcacac gctcgccgtt gacgatgtgg aacaacgtcg
     5401 catcggtcgg cgtgatttcc gcggtaaacg catggttgcg cttggtcagc gtcagcgaca
     5461 acggaccgtc gcctgccgtg cgctcgtacg tcaattgcag atcaagatcg ctgaccggaa
     5521 attcccacgg ctcgggatac acgccgctcg gatgtttcgt gttcggcggc gcgatgtcgt
     5581 agatcaggaa atcgctggtc gtctgcgcgg tgggattggc gttgggctcg aagcggagtg
     5641 tgccgccgcc ggtgttgttg tcgaagccga acacgcggcc gtgcgacatg tcgttcacgt
     5701 tccatccggt gccgtcactg gccgtccacg gcaggtggaa cgacggcacg ttcccgcggt
     5761 ccaggttgtt ctgcgggcga taatcgttgt cgtacaccag ccgccagagc gcatcctgca
     5821 catgcttggg tttggtctga actgtgaaat cgttggcgct cttggcgttg ggggcgcgga
     5881 cataaacatc gccgtcgagg accatgatcg cttcgccggg tttgccgatc aggcgcttga
     5941 tgtagttctg cccgtagttg taccggccgg gacttaccgg gcttttgaag acgacgacgt
     6001 cccagcgcgt cggctgctgg aagaggtaca gatatttcag cacgagaata cggtcgccgt
     6061 aatagaccgc cggattttcg acctgcgacg ggtccatctg aaacccgcag ttggggcaat
     6121 acagcggcag cgtgatccgc ccggcgttgg cgcggatgtc gatgtcatcg ctgtcaccgg
     6181 ccgatgggct ggaccagttg acgtcgaact tgtagccgca atccgggcac tgaaagcgca
     6241 tgtgcgcgcc catgagcgtc ggggccatcg acccggtcgg gatgacgaac gcttcgacga
     6301 cgaacgcgcg gaagatgaac gccagaacga acgcgacgag gatcgattcg atcgtctctt
     6361 tcacgccgcc ggaactgggg tgcggcgacg gtggcgcggc cggcgcatct tgcgccgtgg
     6421 cgggggaggc ggcggaactg ctcgattggg catcatccat ggcgggcgga atattacgcg
     6481 acagcgcaca ggctcgccac gggcaacacc gaaatcagcc cgttccgata atcgcccccc
     6541 cgagcgcccg cgcgcgttac gccagcgatt tgacgtgcgt gcggtaagag cccggcgtgc
     6601 ggcccagccg ctgttggaac accgccgtca tgtacgcctg cgatttgaac ccgctctttc
     6661 gtgcgatcgc cttgatctcc agcgtcgtgt cgtgcagcag ttcgatcgcg cggttcaggc
     6721 gcagccgaat gatctcttcg tggatcgatc gtccgaccag cttgcggaac cgccgctgca
     6781 ggaccgttcg agagaccgcg atggagccga cgatgtcatt gatgcccacg ttcgtgtaag
     6841 cgcgctcgcg aatgacttgc agcgcgcgga ccacgtccgg atcctcgatc gccatcatat
     6901 ctgtggacgc gcgcggcgcg atgcccgcgg gttcgatcag caccgcctcg gtcgggccgg
     6961 gtttgccttg catgaggtgg tcgagcacct ccgccgccac gtagccgcgg gcaacgtgat
     7021 tggccatcac gctggacatg ggcggggagg cgatccggca aatggtttca tcgttgtcca
     7081 cgccgaggac cgcgatctcc tcgggcacgc tgatgcccgc gagtaaacag gcctcgagca
     7141 ggttctggcc catcaagtct gaggacgcca taactccggc aggtttgggc aacgagtcct
     7201 tcagccactg cgccagctcc tgttgttgtt tctcccaccc ctgtgccgcg ccaatgtgat
     7261 tgatctggag ccgggtgctg tagatggcac acggcaagcc acgtgctttc aaggcggcaa
     7321 cgaacgcttc gcgcctgccg cgcgaccatt cgaattcgat attgtcgtag aacgcgaagt
     7381 gcttgaagcc gcggtcgagc agatgctctg cgccgactcg gccgatggct tcgttgtgca
     7441 aacagacgag cggaattcct ttggtgttca ggtggccggc caggtccacg atgggcacgc
     7501 ggcattccct cagttcctcg tccctgaccc tcggtgaata caccatgatg ccgtcgccct
     7561 gccagtcgcg aaaccagttc ggtggcgcct ccgagccata ttgtttgagg taaatggccc
     7621 acgggtcgtg ctcctgcacg taatccgcaa tgccgcgtag catttctgtg acgggggaga
     7681 agcccaagcc cacgatgatc gccacatgct ttggcttgcc cgtgctcggc ggcgagtcgc
     7741 tcgctcgtcg tgcgtccttc aggttcgtca tttcagatgc tttcccgtgt cgtcggccgc
     7801 gcctgttttc accgctcgcc agcggatgtt tagaatgacc cttcgcatca aattgcattg
     7861 aacgcggtcg tcttttacac tgcacgactc agattaccga aggcaccgag cactcgatac
     7921 taacagagat tttgaaagga ccgcagccat gccgttgatg accaccaagc cgatgttcga
     7981 cctcgcctat gaaggcggct tcgccgttgg cgcgtttaac gtgaacaata tggagctcgc
     8041 ccagggcatc atcgaagcct gtgcggccga gaagagcccg ctgatcctgc agatctcgaa
     8101 gggcgcccgc aagtacgcga agatgcatta cctgaaggcg atcatcgatg cggccgtggc
     8161 ggaatatccg tggctgccga tcgcgatcca tcttgatcat ggcgatacgg tggacctggt
     8221 gaagacctgc atcgcggatg ggttcacgag cgtcatgatc gatggctcgc atcatccgta
     8281 cgatgagaat gtaaaagtga cggcggaggc ggtgaaggtt gcgcatgcat ccggtgttac
     8341 cgtcgaggcg gagctgggga tgctcggcgg gattgaggaa gatgtcgtcg gcatggatgc
     8401 tcacgagtat
//
LOCUS       DATIMH010000517         4515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3299686, whole genome shotgun sequence.
ACCESSION   DATIMH010000517 DATIMH010000000
VERSION     DATIMH010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3299686"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VLI90_09045"
     CDS             complement(<1..702)
                     /locus_tag="VLI90_09045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV14393.1"
                     /translation="MLAVAIATVSTVYLAWTAKQQGRALYPVVHDEFSYLIGAQMLAR
                     GRLWMPPHPLAHFFDSFQLIVDPVYASAYFPGTALLFALPVLAHIPIWLFAAAMAGII
                     VALLYLLVTRLIHGTVGLLAAGLLLSAGMFRTLSVMVMSQVPVMLFGLIALLAWFNWR
                     QSRRSRWAMLLGIASGWALITRPIDAICFLLPIGIAALIERRHLPRLICWTAIGLAPF
                     LLIQLSSNKGITGHFF"
     gene            complement(1142..1774)
                     /gene="folE"
                     /locus_tag="VLI90_09050"
     CDS             complement(1142..1774)
                     /gene="folE"
                     /locus_tag="VLI90_09050"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HSV14394.1"
                     /translation="MPPLREDASDARPANMPPANVDIPRIEKAVREILFAVGEDPDRE
                     GLLKTPNRVARAYGELMAGLQDDPRRHLKTVFHERYDEIVLLRDIEFHSLCEHHLLPF
                     TGKAHVAYLPDGKVVGLSKLARLVEGYARRPQVQERLTTEIADALMDELQPIGAACIV
                     EATHTCMTIRGAKKHGSTMVTSALRGIFKENPSSRAEVLSLMYGSRVARP"
     gene            1862..2677
                     /locus_tag="VLI90_09055"
     CDS             1862..2677
                     /locus_tag="VLI90_09055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV14395.1"
                     /translation="MAGISNSVVIVNFNSRVALLALLKSLALHEATSTEVIVVDSAST
                     DGSVEAAREACPQAKVMRLETNRGFYAAVNKGIDRAEGDVAIVCHADVIADIHTLAEL
                     GDQARETEGRKVAAVVPRLVGVDGAEQPFVATLPKLATGVAGALNPVAAWQSRVPFLD
                     HLAENEWARFACVALSQNYLATTGAFDPRFFLYYGDADQCSRIHAKHLRVLISKTVKV
                     THAGASLAKDFPPHLLRILRKDQEQYAQKHFPSWQQGVVRAAGTVGRWFANSD"
     gene            complement(2746..2955)
                     /locus_tag="VLI90_09060"
     CDS             complement(2746..2955)
                     /locus_tag="VLI90_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14396.1"
                     /translation="MGSFAGIGCNIGPNAYIQCYEDLNFFDSVFWLLPDQRVEDTAQL
                     ACNDDIDSLRLFDRPPFAHEPAYTA"
     gene            3013..3354
                     /locus_tag="VLI90_09065"
     CDS             3013..3354
                     /locus_tag="VLI90_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSV14397.1"
                     /translation="MMGPLHSAKRSDPILRQRRLLADLCRLVGMSVGSVEPQALHTDR
                     SERAPLDVNGELSPGLEQTLRHLLAGASEKQVAGHMKLSRHTVHVYMKALYRRYGVSS
                     RAELLAKHLHP"
     gene            3510..4283
                     /locus_tag="VLI90_09070"
     CDS             3510..4283
                     /locus_tag="VLI90_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV14398.1"
                     /translation="MEKTQHQLDSLKQQLTESQRLATIGTIAAVIAHEFNNLLTPIVS
                     YSQFALQSAESATPDIELIKKALGKSYTASSKAGKICNSMLSLARGESTFGAVSVQRL
                     VDEVLMVLARDPQKDGIALRVQVPPDLYIDGDEVQLEQVLLNLLINARQAMLGKGGSL
                     WVKAMRTDDAGEMRIQVIDTGPGIPEKLLPKIFQPFFTTKGTTKKGETKGTGLGLAIC
                     KEIIEHHRGRIEVQSEVGKGTTFSIYLPAAKVTNQPLAA"
BASE COUNT          909 a         1341 c         1492 g          773 t
ORIGIN      
        1 gaagaagtgc ccggtgatgc ctttgttcga gctcaactga atcagcagga acggagcgag
       61 gccgatggcc gtccagcaaa tcaggcgcgg cagatgtcgg cgctcgatca acgcagcaat
      121 gccgatgggc agcaggaaac aaatcgcgtc gattgggcgg gtgatgaggg cccagccgct
      181 ggcgattccc aggagcatcg cccagcgcga tcggcgcgac tggcgccagt tgaaccacgc
      241 caggagcgcg atcagcccga acagcatcac cggcacctgc gacatgacca tcactgagag
      301 cgtgcggaac atgcccgccg acaagagcag tcccgccgcc agcaagccga ctgttccatg
      361 gatcaggcgc gtcaccagca ggtagagtaa tgcgacgatg atgccggcca tcgccgcggc
      421 gaatagccag attggaatgt gcgcgagcac cggcagcgcg aagagcagcg ctgttcccgg
      481 gaaatacgcc gacgcgtaga cgggatcgac gatcagctga aatgagtcga agaaatgggc
      541 gagcggatgc gggggcatcc acagtcgccc gcgggcgagc atctgcgcgc cgatcaggta
      601 gctgaactca tcgtggacga cgggataaag cgcccgccct tgttgcttgg ccgtccaggc
      661 gaggtaaacc gtcgacacag tcgcgatggc gacggcaagc attctttgcg tccgtggctt
      721 gggatgacgc aggggagcgc tcaacgaatt gccgatggcg cgtgtttggg ggatcgcggc
      781 aaggagtgcc gcaatgactg cgagcaacag cagggcacgc ggtccgtcca atccgagcgt
      841 cagaaaaacg ttccagtgtg gcccgaacca cgcccaccat acggccacag cgacgatggt
      901 cgccatcgcc gctcgcgccc gcggaaaggt ccccgatggt ttcgccgcca gcgtcgttgg
      961 atgttcagcg gcttgaacca tggtcgtctg cgttcagccc atgcggcagc ccgcttcaga
     1021 ggcaggcgcc cagcttcccg catctctggc ggcgaggtag cgtgatgagg ccgacgttgg
     1081 cgggcgcgca tcagcggcat cgacgcgcgc gaagctaagt caatgacgat cgcaggtcta
     1141 cttatggtcg cgccacgcgc gagccgtaca tcagcgacag cacttccgcc cggctggacg
     1201 ggttttcctt gaagatgccg cggagggcgc tggtgaccat cgtgctgccg tgcttcttgg
     1261 cgccgcggat cgtcatgcag gtgtgggtgg cttccacgat gcacgcggcg ccgatcggtt
     1321 gaagctcgtc catcagcgca tcggcgatct ccgtcgtcag ccgttcctgc acctgcgggc
     1381 gccgcgcgta gccttcgacg aggcgagcca gtttgctcaa cccgacgacc tttccatccg
     1441 gcagatacgc cacgtgcgcc ttgccggtga acggcagcag gtgatgctca cagagcgagt
     1501 ggaactcgat atcgcgcagc aacacgattt cgtcgtagcg ctcatggaac acggtcttga
     1561 gatggcgacg cggatcgtcc tgcaggccag ccatgagttc gccataggct cgcgccaccc
     1621 gattcggcgt tttcagcagc ccttcgcgat ctggatcctc gcccacggcg aagagaatct
     1681 cgcgcaccgc cttttcaatg cgtgggatat ccacattggc cggcggcatg ttggcggggc
     1741 gggcgtcgct ggcgtcctca cgaagtgggg gcatgtcatt tgctccgatc aatctcagcc
     1801 gtggtgttca aaggttccag ttcttgcggc agcgattata tcctcaatcc tgggagtccg
     1861 cgtggcaggt atttcaaatt cagtcgtgat cgtcaatttc aacagccggg tggcgctgct
     1921 ggcgttgttg aagtcactcg cgctgcatga ggcgaccagc acggaagtga tcgtcgtcga
     1981 tagcgcttcg accgacggca gcgtcgaagc ggcccgcgaa gcttgtccgc aggcgaaggt
     2041 catgcggctg gaaaccaacc gcgggttcta cgcggcggtc aataagggga tcgaccgcgc
     2101 cgagggcgac gtcgccatcg tgtgtcacgc cgacgtcatt gcggacattc acacgctggc
     2161 ggagttgggc gaccaggcgc gcgaaaccga aggccgtaaa gtggcggcgg tggtcccgcg
     2221 gctggtgggg gtcgatggcg cggagcagcc gttcgtggca acgctgccga aactggcgac
     2281 cggcgtggcc ggcgcgctga acccggtggc ggcgtggcag tcgcgcgtcc cgtttctcga
     2341 tcacctggcg gagaacgagt gggcgcgctt cgcgtgcgtc gccctcagtc agaattacct
     2401 cgcgacgact ggcgcgttcg atccgagatt cttcctttac tacggcgacg ccgatcaatg
     2461 ctcgcggatt cacgccaagc acttgcgcgt gttgatcagc aagacggtga aggttacgca
     2521 cgccggggcg tcgctggcga aggatttccc gccgcacctg ctgcgcatcc tgcggaagga
     2581 ccaggagcag tacgcccaga agcattttcc gtcctggcag cagggcgtgg ttcgcgccgc
     2641 cggaacggtg gggcgttggt tcgcgaactc ggattgacga gctgcgggcg cgaggcgcgt
     2701 cgatcgagcg cgcgacgcgt gagatggctc gccgcccaca gcatgctatg cggtgtatgc
     2761 cggttcgtgc gcgaacggtg gtcggtcgaa caggcgcagc gaatcgatgt catcgttgca
     2821 cgccagctgc gctgtatctt ccacgcgctg gtcgggcagc agccagaaca cactgtcgaa
     2881 aaaattgagg tcctcgtagc actggatata cgcgttgggg ccgatgttgc aaccgattcc
     2941 tgcgaatgaa cccaatgtga cggcgccggc tcccgctgcg gccgccaagg ggatgtgtcg
     3001 gcgatgctcc ggatgatggg gccacttcac agcgcgaagc gctcggatcc gatcctgcgc
     3061 caacggcgat tgctggcgga cctgtgccga ctcgtcggca tgagcgtcgg atcggtcgaa
     3121 ccgcaagccc tgcacaccga tcgaagcgaa cgcgcgcccc tcgatgtgaa cggggaattg
     3181 tcgcctgggc tcgagcaaac tttgcggcac ctgttggccg gcgccagcga aaagcaggtg
     3241 gcggggcaca tgaagctgag ccggcacacg gtgcacgtgt atatgaaggc gctctatcgc
     3301 cgctatggtg tgagcagccg cgctgaattg ctggcgaagc atctccatcc ataggctcgg
     3361 gagcgcgccg cacgtctgat aaacgcggcc ccaaacgggt cacaatgacc gcgtgaaggc
     3421 acgcccattt ttcagccgat aacttccgta ccgtgagcga aagccaaatc ccagcggcag
     3481 tttcgatcgg ggacgcgatc acaaatgaat tggaaaagac gcagcatcag ctggattcgc
     3541 tcaagcagca gctgaccgaa tcgcagcggc tggcgacgat cggcacgatc gcggccgtca
     3601 tcgcgcacga gttcaacaac ctgctcacgc ccatcgtcag ctacagccaa ttcgcgctgc
     3661 agagcgccga gagcgctacg cccgacatag agttgatcaa gaaagcactg gggaagtcgt
     3721 acaccgcatc gagcaaggcg ggaaaaatct gcaattcaat gctgagcctt gcccgcgggg
     3781 aatccacctt tggcgcggtc agcgttcaac ggctggtcga tgaagtgctg atggtgctgg
     3841 cgcgcgatcc gcagaaagac ggcatcgcgc tgcgcgtgca ggtgccgccg gacttgtaca
     3901 tcgatggcga tgaagtgcag ctggaacagg tgctgctgaa cctgttgatc aatgcgcgac
     3961 aggcgatgct cggaaagggc ggatcgctgt gggtgaaagc gatgcgcacc gatgacgcgg
     4021 gcgagatgcg gatccaggtg atcgacaccg gcccgggcat ccccgaaaag ctgctgccga
     4081 aaatctttca gccgttcttc acgaccaaag gcacgacgaa gaagggcgaa acgaagggca
     4141 ccgggctggg cctggcgatc tgcaaagaga tcatcgagca ccaccgcggc cggatcgaag
     4201 tgcagtcgga agtgggcaag ggaacgacgt tcagcatcta tctgcccgcg gcgaaagtga
     4261 ccaaccagcc gctcgcggct tagcgtccgt ttcttcgcgt ctactaatag ccgccgcact
     4321 tgtgcggcga ttgtttcccg agaagagacg ccgcacaagt gcggctgcta agactaagaa
     4381 cctatctcaa gtcacgacgt tctactctga acggtcgtaa cgagaggtac cacggatggc
     4441 aagaaagcag aaggcggcgg agccgatgcc gacgatttgg catgcgagcg atgagctttg
     4501 gagcgaggtc gagca
//
LOCUS       DATIMH010000518         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4455390, whole genome shotgun sequence.
ACCESSION   DATIMH010000518 DATIMH010000000
VERSION     DATIMH010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4455390"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..1654)
                     /locus_tag="VLI90_09075"
     CDS             complement(344..1654)
                     /locus_tag="VLI90_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HSV14399.1"
                     /translation="MVPTPSLETAAPPASTSTSRNILFVTAGRVGLLAAWFAATMLLA
                     RVLGPIAFGLYTLAQNAIRIVTNCVGDPLDMAVMRDAPLYLRDDRPRALDVIHSAFWL
                     RVALGVLSIALAAAAPSLIARVVFDDPALTHLALWTATGILGDLLLRSALGYFQVAER
                     FGPFMLVDVVWQVGRAAAAVVLVVMHLISTQTAIMLYVIAPYVAFLVALIMLPRDVRA
                     IAARPPRGRMLDVVHYSKWMAAAMMLGAVYERLDLFLLSWFRGPRDVGIYAGAMALAV
                     IPDFLNGAIQTVLAPKVAPAHARGEFAALQRRYFTYALPLGVIAIAAAWLMGGPIIRA
                     FLSAKYGDSILAFKILIVGTIFNIAATPLSEALLNFTAPKKVMVLTAAGLAIVAGAGV
                     VTIPRYGVTGAACVIVVARVIVGAAVILSPGGTPPESKDLAGNK"
BASE COUNT          338 a          558 c          612 g          271 t
ORIGIN      
        1 gcgcgcagct gcgcgagatg ctcggcaagg acatcggtaa gcgcgatcgg ctgccgtata
       61 cgccgcggtt tgatcagctg gtggatgagt tcaacaagac gcaggggagg ccgctgtcgc
      121 cgcatctggt gtggcggctg gtggcgacgc tggcgaaatg aacggtcttt atcctgagcg
      181 caggcggtac tccgccggag tcgaaggatc ttgccgagaa gtgggccaag cgtcatccgc
      241 gcattcggcc cagtagccgc ttgcggcttc gcaaatcaag catcgcggct tgtccgtctg
      301 cgaagccgca agcgacgctc gcgccgtttg ctccattcac cgttcacttg tttccggcca
      361 gatccttcga ctccggcggc gtaccgcctg ggctcaggat gacggctgct cctacgatga
      421 cgcgcgcgac gacgatcacg cacgcggcgc cggtgacgcc gtagcgggga atcgtgacca
      481 cgcccgcccc ggcgacgatg gccaagcccg cagccgtcag gaccatcacc ttcttcggcg
      541 cggtgaagtt cagcagcgct tccgacagcg gcgttgcggc gatgttgaag atcgtgccga
      601 cgatcaggat cttgaacgcg aggatggagt cgccgtattt cgcggataga aatgcgcgga
      661 tgatcggccc gcccatcagc cacgcggcgg cgatcgcgat gacgccgagg ggtagggcgt
      721 acgtgaaata ccgccgctgc aacgcggcga attccccacg ggcatgcgcg ggggcgactt
      781 ttggcgcgag caccgtttgg atcgcgccgt tgagaaagtc cgggatgacg gccagggcca
      841 tcgcgccggc gtagatgccg acgtcgcgcg ggccgcggaa ccagctcagc aggaaaaggt
      901 ccagccgctc gtacacggcc ccgagcatca tcgcggccgc catccacttg ctgtaatgca
      961 caacgtccaa catccgcccc cgcggtggcc gggcggcgat cgcgcgcacg tcccgcggca
     1021 gcatgatgag tgcgacgagg aacgcgacgt atggcgcgat cacgtacagc atgatcgcgg
     1081 tctgtgtgct gatcaggtgc atcaccacga gcacgacggc ggccgcggcg cgaccgacct
     1141 gccagacgac atcgaccagc atgaacggcc cgaatcgctc ggcgacttgg aagtagccca
     1201 gcgccgatcg cagcagcagg tcacctagaa tgccggtggc ggtccagagc gccaggtgcg
     1261 ttagcgcggg atcatcgaac acgacgcgcg cgataagcga cggcgccgcc gccgccagcg
     1321 cgatcgacag gacgccgagc gcgacgcgca gccagaacgc ggagtgaatg acgtcgagcg
     1381 cgcgcgggcg gtcatcgcgc agatacagcg gcgcgtcgcg catgacggcc atgtccagcg
     1441 gatcgccaac gcagttcgtg acgatgcgga tggcgttctg cgccagcgtg tagaggccga
     1501 aggcgattgg cccgagcacg cgggcgagca gcatggttgc ggcaaaccac gccgccagca
     1561 ggccgacgcg cccagcggtg acgaacagga tgttccgcga tgtcgaggtg gacgcgggcg
     1621 gcgcagcggt ttcgagggaa ggggttggaa ccacagaatg cacagattac acagattcaa
     1681 aaagagttgt tcgatcttga tccatcgctg cgacgccggc gtctgagggt acgcccgaag
     1741 acccgcattc ttttgtcact cgatccgggc cttcggagt
//
LOCUS       DATIMH010000519         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1283298, whole genome shotgun sequence.
ACCESSION   DATIMH010000519 DATIMH010000000
VERSION     DATIMH010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283298"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1823
                     /locus_tag="VLI90_09080"
     CDS             <1..>1823
                     /locus_tag="VLI90_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14400.1"
                     /translation="DPLFLPPVQLSQLPTSPPTHGWPSLTADAGVPASVPGTAEEYLG
                     DGGGPSSCVKGVTWWYRQVQVPARVSGRILLQFESARQRAEVYIDGKLAGYDAVGNSP
                     FEIDLTELGLAGKTVQLAVRITNPGGNFDWIDYTQFQWGKYTLPMSHAFGGITGRVKL
                     VAVEDVYCADLYLQNTPAMHDVNVLVTVQNAGSSPARHDLKVDVLDGQAVVASKTLAQ
                     QDLPPGESTLSVPISAPDATLWDTENPKLYTARVRLSGGARETDQVQKRFGFRWFTMD
                     GIGSDAVFRLNGKRVFLKTAISWGFWPTNGIYATPEMAEKQIRAAKSLGLNMLNFHRC
                     IGSPVVLEKADELGLLYFEEPGGYVAGGRDEFAQGISREKLLRMVQRDRSHPSLIIYN
                     MINEQWNQYGADKDDALFATHQRDLTQAHAIDPSRIIVYASSWGDPGKEQHVKLHMRP
                     FDNTPYFKGWEDSHRATGPETWLQQYYRDPTHHIGATDDTGEIFYRGEEGAISAPPRL
                     EKIHADLQKLPHPGWDGAVYEQWYQAFESFLDRKHLRDAFATVDALTCAMGEVSLEQQ
                     GRRIEAHRIGNVADGYAVNGWEAELVENHSGIVDCFRNPKA"
BASE COUNT          359 a          562 c          600 g          302 t
ORIGIN      
        1 gatccgctgt ttctcccacc cgtgcagttg tcgcagctgc cgaccagccc tccaacccat
       61 ggctggccat ccctcaccgc cgatgccggt gttcccgcct ccgtgccggg gacagccgaa
      121 gaatatctcg gtgacggcgg cggcccgtcg tcctgcgtaa aaggcgtcac ctggtggtat
      181 cgacaggtgc aagtgccagc gcgtgtgtcc ggtcgaatcc tgctgcagtt cgagtccgcg
      241 cggcagcgcg ccgaggttta catcgacggc aaactcgccg gctacgacgc ggtcggcaac
      301 tcgccgttcg aaattgatct gaccgaactg ggcctggccg gcaaaaccgt gcagctggcg
      361 gtgcgaatca ccaaccccgg cgggaacttc gactggatcg attacacgca atttcaatgg
      421 ggcaagtaca cgctgcccat gagccacgcg ttcggcggca tcaccggccg ggtgaagctg
      481 gtggccgtgg aggatgtcta ctgcgccgac ctgtacctcc agaacacgcc ggcgatgcat
      541 gatgtcaatg tgctggtgac cgtgcagaac gccgggtcat cgccggctcg gcatgacctg
      601 aaggtggacg tgcttgacgg gcaggccgtc gtcgccagca agacgctcgc tcagcaggat
      661 ctgccgccgg gggaatcgac gctgagcgtg ccgatctccg cgccggatgc gacgctgtgg
      721 gacacggaga atccgaaact gtacaccgcg cgcgtcaggt tgtccggcgg ggcgcgcgaa
      781 accgaccagg ttcaaaagcg attcgggttc cgctggttca cgatggatgg catcggcagc
      841 gacgcagtgt tccgcctgaa cggcaagcgg gtcttcctga agaccgcgat cagctggggt
      901 ttttggccga ccaatgggat atacgccacg cccgagatgg ccgagaagca gattcgcgcg
      961 gcaaagagcc tcgggctgaa catgctcaac ttccaccgct gcatcggcag cccggtggtg
     1021 ctggagaagg cggacgagct ggggctgctc tacttcgaag agcccggcgg ttacgtcgcc
     1081 ggcggacggg atgagttcgc gcagggcatc agccgcgaaa agctgctgcg catggtccag
     1141 cgcgaccgca gccacccgag cctgatcatc tacaacatga tcaacgagca gtggaaccag
     1201 tatggcgcgg acaaggacga tgcgctgttc gcgacacacc agcgcgattt gacgcaggcc
     1261 cacgcgatcg atcccagccg catcatcgtc tatgcctcat cgtggggaga ccccggcaag
     1321 gagcagcacg tcaagctgca catgcggccg ttcgataaca cgccgtattt caaaggctgg
     1381 gaggattcgc atcgcgcgac ggggccggag acgtggcttc agcagtacta ccgcgacccg
     1441 acccatcaca tcggcgcgac cgacgacacc ggcgagattt tctatcgcgg cgaagaaggc
     1501 gcgatcagcg cgccgccgcg gttggagaag atccacgcgg atttacagaa gttgccgcat
     1561 cccgggtggg acggggcggt gtacgaacag tggtatcagg cattcgagtc gtttctggac
     1621 cgcaagcatc ttcgcgatgc cttcgcgacc gtcgatgcgc tgacgtgcgc gatgggcgag
     1681 gtgagcctcg aacagcaggg ccggcggatc gaggcacatc gcatcggcaa cgtcgccgat
     1741 ggatatgcgg tgaacggctg ggaagcggag ctggttgaaa atcactcggg catcgtcgac
     1801 tgcttccgca accccaaggc cga
//
LOCUS       DATIMH010000520         6998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3876193, whole genome shotgun sequence.
ACCESSION   DATIMH010000520 DATIMH010000000
VERSION     DATIMH010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3876193"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VLI90_09085"
     CDS             <1..512
                     /locus_tag="VLI90_09085"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV14401.1"
                     /translation="TLSDSSGNYKFLINAPSSATTYRVREVLNSGWRLTSPSGGVQSV
                     TLSSGGQATGKNFGDTQHVLISGNVFNDANGNKTKDSGEVGLSGFRVFVDANHNGILD
                     SGELTTTTDSSGNWAFKTLNAGTYTIRVTKPSGSTSTYSTTTSLPAAFTLASGASKTG
                     INFGEKKIA"
     gene            723..2267
                     /locus_tag="VLI90_09090"
     CDS             723..2267
                     /locus_tag="VLI90_09090"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV14402.1"
                     /translation="MRLKSASTAVIERLESRRLLTTVAVVNQGVAHDSFGDTAVGWTG
                     FLLTVNADAGQTITAMDLGTSADSTRGIFGEMLQDWLPGKGGTLTPTPAANSSDAVNG
                     SNFALDSHLLVMNRVNASSPSEDSDGVHPVGLPPNDASDAWGTGSYLHGAFGITSGSA
                     GGSVALAYIVLPTGASGSYSFDVAQSDGVHHVSGMIAAGASISGTMFSDINGDGVRQS
                     SEPPLVGWGCFIDQNANGAFDPGDVRVYADAAGHYQFDNLAPGTYAISQITPTSWRRT
                     FPAKQIGFPVYTITLGSASVAGKNIGNQQLATISGLMFNDLNGDGIHQSTEPVMPGWG
                     CFLDFNNNGAFDTGTDQRVYADATGTYTFSNLVPGTYHVHQVTPTGWRRTTPTSSTYT
                     LTLKAGGVATARNFAMTQKVLITGNVFNDANGSKTRDSGEAGLSGWRVFIDSNNDGVW
                     GKTEISAVTDSAGNFSFKTLDAGTFIVRVVRPSGWSQTTTQPASFTLTSGQTISGIAF
                     GERQTA"
     gene            complement(2604..5138)
                     /locus_tag="VLI90_09095"
     CDS             complement(2604..5138)
                     /locus_tag="VLI90_09095"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSV14403.1"
                     /translation="MKHNTWQLLENAILETLEGRQMMSASPVSPAMLGSDGVLTVSAD
                     AGKSAKMIVDFTPDGKRIQVRAAAQTQTFAVSQVKKLVLNGSSQADYIYVDPRITIPA
                     DIHAGAGNDVIRGSSGDNHIDAGDGNDFVITRNGNNVVDGGAGNDTILAGSGNDHIDG
                     GDGNDFIVAGAGDDTVYGGAGNDTLMGGAGNDVVHGDEGNDLELGGIGNDAVTGDSGN
                     DIVFGNEGTDSVDGGSGDNQIDNPKLMPPAVGPVKNPTTNPTGGPTTNPTGGPTTNPT
                     GGPTTGPTPTPTPTGDNGGAGGPNITVNPNATAPVPVLDVLDGIRQTGLSVNVNALNS
                     QLNAGTPLTAKFEWNFGDDGSKYNDIVAYNGGHVYDTPGTYTITLTVTNEAGGQAVAT
                     QQLTITAAQRTQIFVDAVNGDDTAAGTQAAPIKTIARGAQMLGDNSELLLKAGGKYDM
                     TATAGVGHKNVLVSRYGQGNDPVINRIYGNGTSTFATYGADGITFEHLTFDSPYAAGA
                     DGAAPKVGVSGLYLSGKNITVRNCTFLNIDDAVNEIGNPQGVIVQDNTAPLATGVRGY
                     FVWAQGKQQLIIGNTAVNSTREHIVRMVDVDTSTLEFNDFDNADRGTIDKYDGSKGDI
                     EIHRGQYVYVAHNKIADGDIRTGPLGLWGEDPSTSTQWVVIEDNTLTNTLVYMRPDSL
                     HIMARSNVIMTNVGQAFTIDGTGCKDITIMNNTALDTATSGNFLRVNSHASGIVMTNN
                     LWVAPNIVPGSGACAAVYVNEADLSSFTTIANNIWPAPAKILGWAQGGVNFVGTSFIS
                     SGFQTPDEWSAFSQVHNDSFENAQLTDSYQVVFNGQAVGSALKMAA"
     gene            5801..>6998
                     /locus_tag="VLI90_09100"
     CDS             5801..>6998
                     /locus_tag="VLI90_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14404.1"
                     /translation="MFFDRFGKSVPSSSVAPLTLSQPKDAVVHSGRTSSSSPSSSSHR
                     RKVDDDAIAVRRRRAVALGLAVNLALPMSALTQFGNSGRARAAERPLDGSTPVGTPTK
                     LPSRVLTSSTTLNRSSSTAVMPSYTAPANHSQRALAAPAAAVLPGGPLILASASAPNQ
                     DQFNKGVQQYNAGQYEEALTTLQQVSADGLSDADKKTLSDTLAHAESASAQRKAARAE
                     FQLGEKALADKQYNEAAKHYRNAASNGFADSGTRAKANEQYALAESTAKDAGVDPKSL
                     YEKGKAAYDKGDWTAARQNLTAARDAGYQPGLFNESPQSLLDKVNAKAPAETAAAPTP
                     AAPAKTLVVETPPAPTTPAPTAVTPAPTDNRVALQTEPTVTPAPAPTPAPEATTPPPP
                     APTPAPE"
BASE COUNT         1238 a         2348 c         2183 g         1229 t
ORIGIN      
        1 cgacgctcag cgattcgtcc ggaaactata agttcctgat caacgccccg agcagcgcga
       61 cgacctatcg cgtgcgcgaa gtgctcaata gcggctggcg gctcacgtcg ccgagcggcg
      121 gtgtccagtc ggtcacgctc agctccggtg ggcaggccac cggcaagaac ttcggtgaca
      181 cccagcacgt gctgatcagc gggaacgtct tcaacgacgc caacggcaac aagaccaagg
      241 acagcggcga agttggcctg tccggtttcc gcgtgttcgt cgatgccaac cacaacggca
      301 tcctcgactc gggcgaattg acgacgacca ccgactccag cggcaactgg gcgttcaaga
      361 cgctcaacgc cggcacctac acgatccgcg tcacgaaacc gtcgggttcg acctcgacat
      421 attcgacgac gacctcgctg ccggccgcgt tcacgctcgc cagcggcgcg agcaagacgg
      481 gcatcaactt cggcgagaag aagatcgcct aaggcagatt gacatttagc gcaacgcgga
      541 cgtcccgctc ttgccgagaa gtttctcgtg cacgagcggg acgttctcgt aatccccgcc
      601 cgcacaatcc acacaaatgt cccgaatcaa cgcctggcca tccgatgtcg cttgggcgcg
      661 cggaatccgt ttgcgcttga tggtcgcgaa tcgatatcct ctgcgtgaat cggaggcctt
      721 ggatgcgatt gaagtccgcg agcacggcag tcatcgaacg cctcgaatcg cgtcggttgt
      781 tgacgacggt tgccgtcgtc aatcaaggcg tcgcccacga cagcttcggc gacaccgccg
      841 tgggctggac cggctttctc ctgacggtca acgccgacgc ggggcagacg atcacggcga
      901 tggatctggg tacttcggcc gacagcacgc gggggatttt cggggagatg ctgcaggact
      961 ggctgccggg caaaggcggc acgctcacgc ccacgccggc cgccaactcg agcgacgcgg
     1021 tcaacggcag caatttcgct ttggacagcc acctgctggt catgaaccgc gtcaacgcga
     1081 gcagcccgtc ggaagacagc gacggcgttc atcccgtcgg cctgccgccg aacgatgcat
     1141 ccgatgcctg gggaaccggc tcgtatctgc acggcgcctt cgggatcacc tccggcagcg
     1201 ccggtggcag cgtcgcgctg gcgtacatcg tgttgccgac cggcgccagc ggcagttact
     1261 ccttcgacgt cgcacaaagc gacggcgtcc atcacgtcag cggcatgatc gccgccgggg
     1321 cctcgatctc cggcacgatg ttcagcgata tcaacggcga cggtgtgcgg cagtcgagcg
     1381 aaccgccgct ggttggctgg ggatgcttca tcgaccagaa cgccaacggc gcgttcgatc
     1441 ccggcgacgt gcgcgtctac gccgacgcgg cggggcatta ccaattcgac aacctcgcgc
     1501 ccggcacgta cgcgatcagc cagatcaccc caaccagttg gcggcggacg ttcccggcaa
     1561 agcaaatcgg cttccccgtc tacacgatca cgctgggcag cgcgagcgtg gccggcaaaa
     1621 acatcggcaa ccagcagctg gccacgatca gcggtttgat gttcaacgac ttgaacggtg
     1681 acggcatcca ccagagcacc gagccggtga tgccggggtg gggctgcttc ctggacttca
     1741 acaacaatgg cgctttcgac accggcaccg accagcgtgt ctacgccgac gcaaccggca
     1801 catacacgtt cagcaatctc gtgcccggca cgtatcacgt tcaccaggta acgccgaccg
     1861 gctggcggcg cacaacgccg acgtcgtcca cctacacgct aacgctaaaa gccggcggcg
     1921 ttgcgacggc ccgaaacttc gcgatgacgc aaaaggtgct gatcaccggc aacgtcttca
     1981 acgacgccaa cggcagcaag acgcgcgata gcggcgaggc cggcctgagc ggctggcgcg
     2041 tcttcatcga cagcaacaac gacggcgtgt ggggcaagac cgaaatcagc gccgtgaccg
     2101 acagcgccgg caacttctcg ttcaagacgc tcgacgccgg caccttcatc gtccgcgtcg
     2161 tccgccccag cgggtggagt caaacgacga cccagccagc cagcttcacg ttgaccagcg
     2221 ggcagaccat cagcggcatc gccttcggcg agcgccagac cgcgtgagtc caagcgcgcg
     2281 gccggctgac tctccggtgc cctctcccat gtactcgtgc tgagggtccg actcttttgc
     2341 agaagtggtc aggaaccgat ccacgccggg ttcggagtac ctccgaaccc ggcgtggcga
     2401 gcgtcgatgc gcctcgcatc gggcgatgga aaaactgggg tttcgctggc tgttccccaa
     2461 aagtgttggg tcgtcagtgt actcctcgga gagggggaaa ggcgctcctt gcgcggcacg
     2521 ttcactccaa agaaaaaccg cgggcgctcg gttaggagcg gccgcggttc acttttgctt
     2581 cgccgaaatc aggtcggctg aagttacgcc gccatcttca gggcggagcc aaccgcctgg
     2641 ccgttgaata cgacctggta gctgtcggtc agctgggcgt tttcgaagct gtcgttgtgg
     2701 acctggctga aggcactcca ttcatccggc gtctggaaac cgctggagat gaagctggtg
     2761 cccacgaagt tcaccccgcc ctgcgcccag cccaggatct tcgccggggc cggccagatg
     2821 ttgttggcga tcgtggtgaa gctgctgagg tccgcctcgt tcacgtagac cgccgcacac
     2881 gcgcccgaac cggggacgat gttcggcgcc acccacaggt tgttggtcat cacgatgccg
     2941 ctggcgtgac tgttcacgcg gaggaagttc ccgctggtgg ccgtgtccag cgcggtgttg
     3001 ttcatgatcg tgatgtcctt gcaaccggtg ccgtcaatcg tgaacgcctg gccgacgttg
     3061 gtcatgatca cgttgctgcg cgccatgatg tgcaggctgt cgggccgcat gtagaccagc
     3121 gtgttggtca acgtgttgtc ttcgatgacc acccactgag ttgacgtcga gggatcctca
     3181 ccccacaagc ccagcggccc ggtgcggatg tcgccgtcgg caatcttgtt gtgggcaacg
     3241 tacacatatt gcccgcggtg gatttcgatg tcgcccttgg aaccgtcgta cttgtcgatc
     3301 gtcccgcggt cggcgttgtc gaaatcgttg aactccagcg tcgaggtatc gacgtccacc
     3361 atgcggacga tgtgctcgcg ggtgctgttc accgcggtgt tgccgatgat cagctgctgc
     3421 ttgccctggg cccacacgaa gtaaccgcgc acaccggtcg ccagcggggc ggtgttgtcc
     3481 tggacgatca cgccctgagg gttgccgatt tcgttgacgg catcgtcgat gttcaggaac
     3541 gtgcagttgc ggacggtgat gttcttaccg ctgagataca gacccgagac gccgaccttc
     3601 ggcgccgcgc cgtcggcgcc ggcggcgtag gggctgtcga acgtcaggtg ctcgaaggtg
     3661 atgccgtccg cgccgtaagt ggcgaacgtg ctggtgccgt taccgtagat gcggttgatc
     3721 accggatcgt tcccctggcc ataccggctg acgagcacat tcttatgccc cacgcccgcg
     3781 gtggcggtca tgtcatactt tccaccagcc ttcagcagca gctcgctgtt gtcgccgagc
     3841 atctgcgccc cgcgggcgat cgtcttgatc ggcgcggcct gcgtgccggc ggcggtgtcg
     3901 tcgccgttca ccgcgtccac gaaaatctgt gtgcgctgcg ctgcggtgat tgtcagctgc
     3961 tgcgtggcca cggcctgacc gccggcttcg ttggtgacgg tcagcgtgat cgtgtacgtg
     4021 cccggcgtgt cgtacacgtg cccaccgttg tacgcaacga tgtcgttgta cttgctcccg
     4081 tcgtcgccga aattccactc gaacttcgcc gtcaacggcg tgcccgcgtt cagctgcgaa
     4141 ttgagcgcgt tcacgttcac gctcaagccc gtctggcgga tgccatcgag cacatccagc
     4201 accggaaccg gcgcagtcgc attcggattg acggtgatgt tcggcccacc cgcgccaccg
     4261 ttgtcgccgg tcggagtcgg cgtgggcgtg ggacccgtgg tcggcccgcc cgtcgggttc
     4321 gtggtcggcc caccggtcgg attggtggtg gggccaccgg tcgggttggt cgtcgggttc
     4381 ttcaccggac ccaccgccgg cggcatcagc ttgggattgt cgatctgatt gtcaccgctg
     4441 ccgccatcga cgctgtcagt gccttcgttg ccgaagacga tgtcgttacc gctgtcgccg
     4501 gtcacggcgt cgttgccgat cccgcccagc tccaggtcgt tgccttcatc gccatgaaca
     4561 acgtcgttgc ccgcgccacc catgagcgtg tcgttgccgg cgccgccgta gaccgtgtcg
     4621 tcgccggcgc cggcgacgat gaaatcgttc ccgtcgccgc cgtcgatgtg gtcgttcccc
     4681 gacccggcca ggatcgtatc attgccggcg ccaccatcga cgacgttgtt tccgttgcgc
     4741 gtgatgacga agtcgttgcc gtcgccggcg tcgatgtggt tgtcaccgct gctgccgcgg
     4801 atcacgtcat tgcccgcgcc ggcgtggatg tcggcgggga tcgtgatgcg gggatcgacg
     4861 tagatgtagt cggcctggct cgacccgttc agcaccagct tcttcacctg gctgaccgcg
     4921 aaggtctgcg tctgggcggc ggcgcgcacc tggatgcgtt tgccgtcggg cgtgaagtcc
     4981 acgatcatct tcgccgactt gcccgcgtcg gcgctgacgg tgagcacccc gtcgctgccc
     5041 agcatcgccg ggctgacggg ggaggcactc atcatctgcc ggccctcgag cgtttccagg
     5101 atcgcgtttt ccagcagctg ccaggtgttg tgcttcatgt gaatcgaatc gggggaaagt
     5161 caaacctcct tcgctgtaga attgcgatat cagtattgat ttcgcaggtt tgacacggtc
     5221 acagccccga aacgcggcag cggcggcaca acttcacgtc cgacaatatt cgggatatca
     5281 atcctgatca tcgcggcgtc ggaatcacga tgcctagccg cccgcagggg cacggtcttc
     5341 tccgtctgac gggaagaaaa cgcgtgcccc ttcggggcga ccgctacgca ttagatgatg
     5401 ctccggcaac caccgcacgc gcaattttat ttgcagcccc ccggggcttc gcttacactc
     5461 cgcccgcttt cgcggcggtc gctcgtccct cggcgggccg ataatcccgg cggcggcaac
     5521 gaagcaaatg tcggcgctct cgcatcttgg cgcgagagac ggtaaaagtt tcccccgcaa
     5581 cggatagccg cgttaacatg ccgcgaatgt cggcggcgcg ggatcgcgac agggacgtga
     5641 ttcacccgcg cgacgcgctg atccgctgac gaacgctcgc gcggagcgag cgaacgagca
     5701 agcgagacat cccggcaaca agcagcagca gagaccacgc accaaaaaat aagcatcaac
     5761 ggattttccc cacgcatccc cgccttactg gaggaacgct gtgttcttcg atcgtttcgg
     5821 caagagtgtt ccgtcgtcgt cggtcgcccc tctgaccctc tcacaaccga aagatgccgt
     5881 cgttcacagc ggccgcacct cctcgtcgag cccctcctcg tcgagccatc gtcgcaaggt
     5941 cgatgacgat gccatcgccg tgcgccgccg gcgcgccgtg gcgctgggac tggccgtcaa
     6001 cctcgcgctg ccgatgagcg ccctgacgca gttcggcaac tccggtcgcg cccgcgcggc
     6061 cgagcgaccg ctggatggtt ccaccccggt cggcacgcct accaagctgc cgtcgcgcgt
     6121 gttgaccagc agcaccacgc tcaatcgatc cagcagcacc gcggtcatgc cgtcgtacac
     6181 cgccccggcg aatcattccc agcgcgccct cgccgccccc gcggccgcgg tgttgccggg
     6241 tggtccgctg atccttgcct cggcttcggc gcccaaccag gaccagttca acaagggcgt
     6301 gcagcaatac aacgccgggc aatacgaaga agccctgacg acgctgcagc aggtgagcgc
     6361 tgatggtctg tccgatgccg acaagaagac gctgagcgac acgctcgccc acgccgaaag
     6421 cgcctcggcc cagcgcaagg ccgcccgcgc cgaattccaa ttgggcgaaa aagccctggc
     6481 tgacaaacag tacaacgaag ccgcgaagca ttatcggaat gccgcgtcga acggattcgc
     6541 cgatagcggc acgcgcgcca aggccaacga gcagtacgcg ctggcggaat ccaccgccaa
     6601 ggacgcgggc gtcgatccga agtcgctcta tgaaaaaggc aaggcggcgt acgacaaagg
     6661 cgactggact gccgcccggc agaacctgac cgccgcccgc gacgcggggt atcagccggg
     6721 attgttcaac gaatctccgc agtcgctgct ggacaaggtg aacgccaagg cgcccgcgga
     6781 aacggccgct gccccgacac cggcggcgcc cgccaagacg ctggtcgttg aaacaccgcc
     6841 agccccgacg acgccggcgc ccacggccgt gacgcccgcg ccgaccgaca atcgcgtcgc
     6901 gctgcagacc gagccgaccg tcacgcccgc cccggcgcca acgcccgcgc ccgaggcaac
     6961 cacgccgccg cctcccgcgc caacgcccgc cccggagg
//
LOCUS       DATIMH010000521         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3300894, whole genome shotgun sequence.
ACCESSION   DATIMH010000521 DATIMH010000000
VERSION     DATIMH010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3300894"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VLI90_09105"
     CDS             <1..1201
                     /locus_tag="VLI90_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HSV14405.1"
                     /translation="PEHPLDSPEFIELLSALADAEQRDSSHVEVLTNGSAFYEAELAA
                     IAAAKRSINLEAYIFQKDQIGRRFVDALAARARAGVRVNVVVDAIGSIATWDKTFAPL
                     REAGGRVCWYQPIRWYTLKRFNTRTHRELLIIDGEVGFIGGAGIGDNWLTGTREDPPW
                     RDTVVRIHGDIVVGLQTTFAENWLESADEILTGDEYFPRQRWRGGEQEPASDDPNAAT
                     GMVVISSPSAGRSTRGRVLFQTLLASAKQSIHINSPYFLPDRSVRRELIDAVKRGVKV
                     SVITPGKHSDHMMTRSASRRRYGELLAGGVTIHEYSPAMIHVKALVIDEIWSVVGSTN
                     FDNRSFGLNDEVNLAVRCRPLARRFDEDFARDLEQSHCMTLEEWNRRSIGERVMEMLG
                     RVIERQQ"
BASE COUNT          299 a          490 c          546 g          297 t
ORIGIN      
        1 gccggagcac ccgctggatt cgccggagtt catcgagctg ctcagcgcgc tggcggacgc
       61 cgagcagcgc gattcgagtc acgtcgaagt gctgaccaac ggcagcgcgt tttacgaagc
      121 cgagctggcc gccatcgcgg cggcgaagcg cagcatcaac ctcgaagcat atatatttca
      181 aaaggaccag atcggccggc ggttcgtgga tgcgctggcg gcccgcgctc gcgcgggcgt
      241 gcgggtgaac gtcgtcgtcg atgcgatcgg cagcatcgcg acgtgggaca agaccttcgc
      301 cccgttgcgc gaggccggtg gacgcgtctg ctggtatcaa cccatccgct ggtacacgct
      361 caagcggttc aacacccgca cgcaccgtga actgctgatc atcgacggcg aagtcggttt
      421 catcggcggg gccggcatcg gtgacaactg gttgaccggc acgcgcgagg accctccctg
      481 gcgcgacacg gtcgtgcgca tccacggcga catcgtcgtc ggactgcaga cgacctttgc
      541 cgagaactgg ctcgaatcgg cggacgagat cctgactggt gatgaatatt ttccgcgcca
      601 gcgctggcgc ggcggtgagc aggaacccgc cagcgacgac cccaatgcgg cgacggggat
      661 ggtggtcatc agttcaccct cggccgggcg atcgacgcgc gggcgcgtgc tgtttcagac
      721 gctgctggcg tcggcgaagc agtcgatcca catcaactcg ccgtatttcc tgccggaccg
      781 cagcgtgcgg cgcgagctga tcgacgcggt gaaacgcggg gtgaaggtca gcgtgatcac
      841 accggggaaa cacagcgatc acatgatgac ccgcagcgcc agccggcggc ggtatggcga
      901 actgctcgcc ggtggcgtga cgatccacga atactcgccg gcgatgatcc acgtgaaagc
      961 gctggtgatc gacgagattt ggagcgtggt gggatcgact aattttgata accgctcgtt
     1021 cgggttgaac gatgaggtga atttagcggt gcggtgccgg ccgctggcgc ggcggtttga
     1081 tgaggatttc gcgcgcgacc tggagcagtc gcactgcatg acgctggagg aatggaatcg
     1141 gcggtcgatt ggggagcggg tgatggagat gctggggcgg gtgattgagc ggcagcagta
     1201 attatcctac gttcgtgaat gctaaccgcc gaagcggtta gcccggtcat tcgtacgcgc
     1261 caccgatggc agacttcctt cctgccatct ttggtcatga cgagacggac atcaaccggc
     1321 accggcagca agattgcgca ggatctccga ttgttgctga caggccgctt tcgccgcgct
     1381 tatcagaaaa gatcggcccg gggacggccc ggctctcccc tttgaggcaa tggattcgct
     1441 ttactactac aatacgcgcg gatgaccacc accgccccgc cgccggttga cgacgttgct
     1501 cgcgagtacg acccggactc ctatcgcatg acgatcggcg agcacctgga ggaactgcgc
     1561 acgcggctca tcgtctcgct ggtcggcttc gtcgtcgccg ccattgtctg cctttcgttc
     1621 ggggaacgca tc
//
LOCUS       DATIMH010000522         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3876578, whole genome shotgun sequence.
ACCESSION   DATIMH010000522 DATIMH010000000
VERSION     DATIMH010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3876578"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VLI90_09110"
     CDS             <1..357
                     /locus_tag="VLI90_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14406.1"
                     /translation="VREDGVFTGKISSIRNAGEKILLICEDSKTVSSGSFVADPADWN
                     NSKVVIDLLATRHDLHTKHANNLGNAGSGSANGEGNQDGYGNVVFCDGHGEFMSRKDA
                     LRQRYSGNPNPDPVGF"
     gene            482..1414
                     /locus_tag="VLI90_09115"
     CDS             482..1414
                     /locus_tag="VLI90_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSV14407.1"
                     /translation="MADALPEKSFLVQKFAKLPLGAVHVIGYSVAGEETVVQVPELNV
                     CFDIGRCPYFALTSDVVCITHGHMDHLAGIAYYLSQRYFQGMQPGTIVLPRELERPVD
                     ALLRCWRDVERQGTPYKLVPMSPGQLYEVRRDFGIRAYQTHHGGVSLGYGLISIREKL
                     KAEYMGIAGQELAAMKKRGIEIQYRVEVPLVVFLGDTTAGPVFDESDVQNAEVLITEC
                     TFFDADHRMKAKAGRHLHVDQFAQILPRLRSKHIVLTHVSRRTGIRRAKHLLRKKVGD
                     ELMKNVQFLMDFEGSTDAGDVEEAGPNPPPDMAE"
     gene            1608..>2258
                     /locus_tag="VLI90_09120"
     CDS             1608..>2258
                     /locus_tag="VLI90_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14408.1"
                     /translation="MRPIAFIVAVLLAAGSAFASVDRLVIHEWGTFTSLQDEDGRSIG
                     AINSDDERLPPFVHDLIQSEFQYYKGGVPDSFPSVTMRLETPVLYIHPAPGMKVPPLD
                     VTVHFRGGLLTQFYPLAAASRPRFTNAQLDPITADTDGRLAWAHLTVGGDAAGPATDA
                     HVWTAPRDVAGIANFSANGESEKFLFYRGVGHLDAPVRVVRHSNLLMFQNQVEFPLV"
BASE COUNT          422 a          718 c          702 g          416 t
ORIGIN      
        1 gttcgcgagg atggcgtgtt caccggcaaa atctcgtcga tcaggaacgc cggcgagaaa
       61 atcctgctga tttgcgagga ctcgaaaacg gtatcgtccg gttcatttgt ggccgacccg
      121 gccgactgga acaattccaa agtggtgatc gacctgctcg cgacccgcca cgacctgcac
      181 accaagcatg ccaacaacct cggcaacgcc ggcagtggca gcgcaaacgg tgaaggcaac
      241 caggacggct acggcaacgt ggtgttctgc gacggtcacg gcgagttcat gtcgcgcaaa
      301 gatgcgctgc gtcagcgcta ttccggcaat ccgaacccgg acccggtcgg gttctgatac
      361 ggcagatcgc tgctggccaa ttcgctatcg aatgtccggc cagcgcattc tcgcttcggg
      421 aacttccagc cgtcacataa ttgccgcggc ccccgccccg cctatacttt ccgcacctca
      481 catggcggat gcgttgccgg aaaaatcgtt cctcgttcag aagttcgcca agctgccgct
      541 cggagcggtg cacgtgatcg gctacagcgt ggccggtgaa gagacggtcg tgcaggtgcc
      601 ggagctgaac gtgtgcttcg acatcggccg ctgcccatac ttcgccctca ccagcgatgt
      661 cgtctgcatc acgcatgggc acatggatca cctggcgggc atcgcgtatt acctgagcca
      721 gcgctatttc caaggcatgc aaccgggcac gatcgtgctg ccgcgggaat tggaacggcc
      781 ggtggatgcg ctgctgcgct gctggcgcga cgtggagcgc caaggtacgc cgtacaagct
      841 cgtgccgatg tcgccggggc agctgtacga agtccgccgc gacttcggca tccgcgcgta
      901 tcaaacccat cacggcggcg tcagcctcgg atacggtttg atcagcatcc gcgaaaaact
      961 caaagccgaa tacatgggca tcgccggtca ggaattggcc gcgatgaaga agcgcggcat
     1021 cgaaatccag taccgggtcg aggtgccgtt ggtcgtcttc ctcggcgaca ccacggccgg
     1081 tcccgtcttc gacgagtcgg acgtgcagaa cgccgaggtg ctgatcaccg aatgcacgtt
     1141 cttcgacgct gatcatcgca tgaaagccaa agcgggccgg cacctgcacg tcgatcagtt
     1201 cgcgcagatc ctgccgcggc ttagaagtaa gcacatcgtg ctgacgcacg tgtcgcgccg
     1261 taccggcatc cgccgggcca agcacctgct gcgcaaaaag gtgggcgacg agctgatgaa
     1321 gaacgtgcag ttcctgatgg atttcgaagg atcgaccgac gcgggggatg tggaggaggc
     1381 gggaccgaat ccgccaccag atatggcaga gtgactccat ccttcgcagc cgcttggctt
     1441 tagcccgcgg accgttgagg tttcgaacga tgcccgatgt ccgaacggtc cgcgggctga
     1501 agccccgcgg ctgcaaaaga tcatatcgat cgcgagaact tcctttcgcc ctgcgcttct
     1561 gacggtgtgg tatcctcgtc gggcccttca ccttacccgc aggccgcatg cgccccatcg
     1621 ccttcatcgt tgccgtgctt ctcgccgccg gttcggcgtt tgccagcgtt gatcgcctcg
     1681 tcatccacga gtggggcacg ttcacatccc tgcaggatga agacggccgc tcgatcggcg
     1741 cgatcaacag cgacgacgag cgcctgccgc cgttcgtgca cgatctgatt cagagcgagt
     1801 tccagtacta caagggcggc gtgccggatt cgtttcccag cgtcacgatg cggctggaaa
     1861 cgccggtgct ttacatccat cctgcgccgg ggatgaaggt gccgccactg gatgtgacgg
     1921 tgcatttccg cggtgggcta ctgacgcagt tctacccgct ggcggcggcg tcgcggccgc
     1981 gattcacgaa tgctcaactg gaccccatca cggccgacac cgacgggcgg ttggcgtggg
     2041 cgcacctcac cgtcggcggt gacgccgcgg gtcctgcgac cgatgcgcac gtgtggaccg
     2101 cgccgcgaga cgtcgcgggc atcgccaact tcagcgcgaa tggcgaatcg gaaaagttcc
     2161 tgttctaccg cggcgtgggt cacctcgacg cgccggtgcg ggtcgtccgc cacagcaacc
     2221 tgctgatgtt ccagaaccag gttgagttcc cgctcgtc
//
LOCUS       DATIMH010000523         1782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_705779, whole genome shotgun sequence.
ACCESSION   DATIMH010000523 DATIMH010000000
VERSION     DATIMH010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705779"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..1343)
                     /locus_tag="VLI90_09125"
     CDS             complement(294..1343)
                     /locus_tag="VLI90_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSV14409.1"
                     /translation="MQAVRLHGPRDLRVEELPAPNSPGVGEVLLAVRSVGICGSDLHT
                     YQDARIGDTLLGGPLCLGHEFAGVIEALGDAALDGNRALLRAGMAVAVDPALNCGNCE
                     MCHKGHPNLCPDVVFLGLYPHDGALRQQMIVPASSCFPIAAGVDFGEGALLETLGVAL
                     HAVDLANIRVGDSVALLGAGPIGLCVLQVAKLAGATSIFVSDKFDWRLELAKELGATA
                     VSNCDREDALASVWRATAGRGVDVSIEAAWADHSVQQAADMTRNGGRLVLVGIPGDDR
                     LTLRHSTARRKGLTIRLARRMKHVYPRAIALHASGAVDLRRLITHRFPLAQSARAFDA
                     NLRYETGVGKVVIDI"
BASE COUNT          340 a          627 c          523 g          292 t
ORIGIN      
        1 tgatcgcatc gctcatgagc ttctcctcgc acacttccac caccaccctc cacttgaccc
       61 tgccgccgcg cgtgcgcgat taagaccgca atcgcgcacg acgacatgcg agcgcgctcg
      121 ctgtcggccc gactggtgag gatgatccga acccgcgccc caagaacgat tcccgccgcg
      181 tcggcgttgc cgaggaacgt caactgctta gccaggatgt ttcccgcttc caggtccggc
      241 gccagcagga tctccgcgcg gaaactgttt gggatgattc gttaccgaca gcctcagatg
      301 tcgataacga ccttccccac gcccgtttca tagcgaagat tggcatcgaa cgcgcgggcc
      361 gattgcgcca gcgggaagcg atgggtgatc agccgccgca ggtccaccgc gccgctggca
      421 tgcagcgcga tcgccctcgg gtatacgtgc ttcatccggc gggccagccg aatcgtcagc
      481 cccttccgtc gagcagtgga atgcctcagc gtcaggcgat cgtcacccgg gataccgacc
      541 agcacgagac gtccgccgtt gcgcgtcatg tccgccgcct gctgcaccga atgatccgcc
      601 cacgccgctt cgatcgagac gtcaacgccg cgccccgccg tcgcgcgcca gacgctagca
      661 agcgcatctt cacggtcaca gttggacacc gccgtcgcgc ccaattcctt cgccaactcg
      721 agccgccaat caaatttatc gctgacgaag atcgacgtcg cgcccgccag cttagccacc
      781 tgcaggacgc ataatccgat cggccccgcg ccaagcagcg cgacgctgtc gccgacgcgg
      841 atgttggcca ggtccaccgc gtgcagcgcg acaccgagcg tttcgagcag cgcgccttcg
      901 ccaaaatcaa cgccggcagc aatcggaaaa caactgctcg ctggcacgat catctgctgg
      961 cgcagtgcgc catcgtgcgg atacaggcca agaaacacga cgtcggggca aagattcgga
     1021 tgccccttat ggcacatctc gcaattcccg caattcagcg ctggatcgac cgccaccgcc
     1081 atcccagcgc ggagcagcgc gcgattgccg tcgagcgccg cgtcccccag cgcctcgatc
     1141 actccggcga attcgtgccc cagacatagc ggcccaccga gcagcgtgtc gccgatgcgg
     1201 gcgtcctggt aggtatgcag atcgctgcca cagatgccga ccgatcgcac cgcaagcagc
     1261 acctcaccaa cgcctggcga gtttggcgct ggcagttcct cgacgcgcaa atcccgcggc
     1321 ccatgcagtc gaacagcttg catggacgcg attgttgccc atcgcgttgc gccgatcaaa
     1381 cctgagcgag tacatcctcg taccccgcgt ccgcatttcg ttagggccag gagcgccaag
     1441 acgcggagac gccaagggcg aaagatattg aaatacgcat gagccggaac tgaacgagtg
     1501 atcgcggcga gatgcgcgca gcagcgcgtg aggccgccgg aaagccgcgg ggtgatctgc
     1561 ccgtgcacga agtgctgcca ttgatcctcg gcgatcgcgc tgccgtcgcg gcgggtttgg
     1621 ccgaagtaaa gttccgtgcg gatccagtcc gatccggggg acgcgcgcgc aggacatcag
     1681 tgtcctgcgg cgcttgttgg gccgatgggc gcgccgcagc acaacaccat cagcagcaat
     1741 ccgatcgttg tccttcgcat cgctcgcatc gcttcctcga tc
//
LOCUS       DATIMH010000524         6010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_415203, whole genome shotgun sequence.
ACCESSION   DATIMH010000524 DATIMH010000000
VERSION     DATIMH010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415203"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1863
                     /locus_tag="VLI90_09130"
     CDS             <1..1863
                     /locus_tag="VLI90_09130"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSV14410.1"
                     /translation="LADAMFREVTAHAVTQARDHVLRVVPVARSLAALGDRGLTLNDT
                     QKLAHQLVGLLRANPGVTWISYSDEAGTFTGAHRTASGTLRINQSWIEAGHTKLIEHD
                     VYANDSWSIFRTNDDSKYDPRKRPFYIQARDAGRLVWLPPYIFYDEGVPGITCACPLF
                     DQAGKMLGVLTFDFDLNALSQFAAGLTISQNSKVTLMTPEGVLLAHPSSQMNVTRGHM
                     GEGTLPTVADVDDPAAKVFFEQLRPADVASGPDRYRQFEFNVAGTRYYAGAHSFQVED
                     GPVWIVGAMAPQSDFLAAVYRHNLASLGISAAAVLVAVVLAGLLARRVSEPVVGLVGV
                     MNRVGSGDLDATADLGGSTEFRRLSAALNQMTSDLRDGMRLRSALAVAQEVQQKLLPL
                     TPPNVAGLDVAGHTSYCDETGGDFYDWLVLDRETGGGVLVAIGDVVGHGIGAALLMAT
                     ARGILRSCSLTCDSLGDVASHLNNLLVTDMRGGRFLTLCLALIDPKARTVRWSRAGHD
                     PALIYDPDTGRFDELLGGGTPLGIDENIKYEEFSYAPLKPGQVIALGTDGIWEATNNR
                     GEFFGKDRLRDAIRAAAVGSASDIVLAIRDAIDHFRGDQRQADDITFVVIKMTM"
     gene            complement(1906..2319)
                     /locus_tag="VLI90_09135"
     CDS             complement(1906..2319)
                     /locus_tag="VLI90_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14411.1"
                     /translation="MDWMMIGGCVGLGAVAIGVCASSVFHARSLETGQLARWAKAASV
                     VALLIAGLSLSIYALVVIHQSSFCDPANRRANMVVRWLLAEIFIAFIFFCLIAAISPL
                     LPRDKAADLLHRRSQRVVVVMVICATCCGAIVALF"
     gene            complement(2381..3145)
                     /locus_tag="VLI90_09140"
     CDS             complement(2381..3145)
                     /locus_tag="VLI90_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV14412.1"
                     /translation="MILEQFRIEGKTALVTGAKRGIGKAIAIALAEAGADVIGVSATL
                     EPGGEVERRVVEVGRKFRGYACDFSDRKALYAFIAQVKRDYPRIDILVNNAGTIMRKP
                     AAEHPDEYWDKVMEVNLNAQFILSREIGKEMVARGDGKIVFTASMLTFQGGITVPGYA
                     AAKGGVAQLTKALANEWAGRNVQVNAIAPGYIATDNTAALQADETRNRTILERIPAGR
                     WGKPEDLAGAVVFLCSPAGAYMHGTVLPVDGGWLGR"
     gene            complement(3711..4892)
                     /gene="dcm"
                     /locus_tag="VLI90_09145"
     CDS             complement(3711..4892)
                     /gene="dcm"
                     /locus_tag="VLI90_09145"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HSV14413.1"
                     /translation="MNQTIAAKTFAEFFAGIGLVHRGLEFGGWRCVYANDIEQKKHEM
                     YRALYSGASYYHVQDVWKTSEVASKIPPATLLATASFPCTDLSTAGYYKGLKGEESSA
                     FFGFAKVLQRLRDEERQPPLLLIENVPGLLTSKSGEDFRHVSKELAALDYFLDAVIID
                     AKHFTPQSRPRLFIVGALRDYLPQDSAVLTTPADWLAASAWEDRVSARPDLRPDRLRR
                     AMLECDLPTGWVTYALPRLPKCLIPLTAVVDLDKDQEWWEAESVQKHLKMMSKLHRSV
                     VNELVSRGDRHIGMIYRRRRHGHMRAEVRFDGLAGCLRTPKGGSARQIVIVIDEGQVR
                     MRWMSAVEYARLQGAGDFPIRVPDTQAMFGFADGVCVPVIRWLDESLLSKLAERASVH
                     V"
     gene            4967..5944
                     /locus_tag="VLI90_09150"
     CDS             4967..5944
                     /locus_tag="VLI90_09150"
                     /inference="COORDINATES: protein motif:HMM:NF027605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4928 family protein"
                     /protein_id="HSV14414.1"
                     /translation="MNFSDDLLPLLDEWYERLAMLRGSTQRGQGTYFGALVILQNLIE
                     AYNLDPSGHFTDEGEGGRISGTSAERVESILAALDPRIQVNQYAGRTSTGPTKAARIL
                     LQVFQRAGLDRVNGEERGQLLHLCQKYMCDRLWQLQAGRPIYFVLDPALTLKQNLARL
                     IEQQREYERDRAVAQHLVGAKLELRFPRKHYPDVIINRDSVDAADASEQKPGDFVVGD
                     SVFHVTIAPTKKHFEKCEQNVLSNLRPILLVPEIQLAAVAGALRLQYSTKIDVFSIES
                     FIAQNVEELSAFSYGSMRSRVRDLVQIYNDRIDQAERGKPYLRIDVSRL"
BASE COUNT         1198 a         1944 c         1731 g         1137 t
ORIGIN      
        1 ctggccgatg cgatgttccg cgaggtcacc gcccacgcgg tgacgcaggc gcgcgatcac
       61 gtgctgcgcg tcgtgccggt ggcgcgatct ttggccgcgc tgggcgatcg cggtcttacg
      121 ctgaacgaca cgcaaaagct cgcccaccag ctggtcggcc tgctccgcgc caaccccggc
      181 gtcacctgga tcagctacag cgacgaagcc ggcaccttca ccggcgccca tcgcaccgcc
      241 agcgggacgc tgcgcatcaa ccagagctgg atcgaggccg gccacacgaa gctgatcgag
      301 cacgatgtct acgccaacga ttcctggtcg atctttcgca ccaacgacga ttcgaaatac
      361 gacccgcgca aacgcccgtt ctacatccag gcgcgcgacg cggggcggtt ggtctggctc
      421 ccgccgtaca tcttttatga cgaaggagtg cccggcatca cctgcgcctg tccgctgttc
      481 gaccaagccg gaaaaatgct cggcgtgctg acgttcgact tcgatctgaa cgcgctctcg
      541 caattcgccg cggggttgac gatcagccag aactcgaagg tcacgctgat gacgcccgag
      601 ggcgtgctgc tggcgcaccc gagcagccaa atgaacgtga cgcgcggcca catgggtgaa
      661 ggcacgctgc ccaccgtcgc cgatgtcgac gaccccgcgg cgaaagtgtt cttcgaacaa
      721 ctgcgacccg ccgacgtcgc gtcgggaccg gatcggtatc gccaattcga attcaacgtc
      781 gccggcacgc gctactacgc cggcgcgcac tcgttccagg tggaagacgg cccggtgtgg
      841 atcgtcgggg caatggcgcc gcagtcggac tttctcgccg cggtgtatcg gcacaacctc
      901 gcgtcgctgg gcatctccgc cgccgccgtg ctggtcgcgg tggtgttggc tggcttgctc
      961 gcgcggcgcg tctcggagcc tgtcgtcgga ttggtcggcg tgatgaaccg cgtcggcagc
     1021 ggtgatctcg atgcgacggc cgatctcggc ggcagcaccg aattccgccg gttgtctgcc
     1081 gcgctcaacc agatgaccag cgacctgcgc gacggcatgc gcctgcgcag cgcgctggcc
     1141 gttgcgcagg aagttcagca gaagctgctg ccgctgacgc cgcccaacgt ggcaggattg
     1201 gacgtcgccg gtcacaccag ttactgcgac gagaccgggg gagactttta cgattggctc
     1261 gtcctcgacc gcgagaccgg cgggggtgtg ctcgtcgcca tcggcgacgt cgtcggtcac
     1321 ggcatcggcg ctgcgctgtt gatggccacc gcccgcggca tcctgcggag ctgctcgctc
     1381 acctgcgatt cgctcggcga cgtcgcctcg cacctgaaca acctgctcgt caccgacatg
     1441 cgcggcggac ggttcctcac gctctgcctc gcgctgatcg accccaaggc gcgaaccgtc
     1501 cgctggtcgc gcgccggcca tgaccccgcc ctcatctacg atcccgacac cggtcgcttc
     1561 gatgaactgc tcggtggcgg cacgccgctg ggcatcgacg aaaacatcaa gtacgaggag
     1621 ttcagctacg cgccgctgaa gccggggcaa gtgattgcgc tgggcaccga cggcatctgg
     1681 gaagcgacga acaaccgcgg tgaattcttc ggcaaggacc gcttgcgcga cgccatccgc
     1741 gccgccgccg tcggcagcgc cagcgacatc gtcctggcca tcagagacgc catcgaccac
     1801 ttccgcggcg accagcgcca ggcggatgac atcacgttcg tggtcatcaa gatgacgatg
     1861 taaagccggc gatgggatcg ctccgagaga tcgcagtcgc tggctctaaa acaacgcgac
     1921 gatcgcgccg cagcacgtcg cgcaaatgac catcacaacg acgacgcgtt gcgaccgcct
     1981 gtggagcaag tctgccgcct tgtcccgcgg gagcagcggc gaaatcgccg cgatcaagca
     2041 aaagaaaatg aatgcgatga atatttctgc cagaagccag cggaccacca tgttcgcccg
     2101 ccgattagca ggatcacaaa atgaagactg gtgaatgaca accagtgcat aaatcgaaag
     2161 actcaagccc gcgatgagca gtgcgacgac ggacgccgcc ttcgcccaac gcgcaagttg
     2221 gccggtctcc aatgatcgtg catgaaaaac cgatgacgca caaacgccga tcgctacggc
     2281 gcccagaccc acacagccac cgatcatcat ccaatccatt tcgatcccgc gggcacatca
     2341 cgcaattctt aggccgatcg tcgttgaaac gccggcttct tcaccgaccc aaccaccccc
     2401 catccaccgg cagcacggtt ccatgcatgt acgctccggc ggggctgcac agaaaaacca
     2461 ccgcgccggc caagtcttcc ggcttgcccc aacgccccgc ggggatgcgc tccagaatcg
     2521 tccgattccg cgtctcgtcc gcctgcagcg ccgcggtgtt gtcggtcgcg atataccccg
     2581 gcgcgatcgc gttcacctgc acattccgcc ccgcccattc gttcgccagc gcctttgtta
     2641 actgcgcgac tccgcccttc gccgccgcgt accccggcac ggtgatgccg ccctggaacg
     2701 tcagcatcga tgcggtgaag acgattttcc cgtcgccgcg cgcgaccatc tccttgccga
     2761 tctcccggct caggatgaac tgcgcgttga ggttcacctc catcaccttg tcccaatatt
     2821 catccggatg ctccgccgcc ggtttgcgca tgatcgtgcc tgcgttgttc acgaggatgt
     2881 cgatccgcgg gtaatcgcgc ttcacctgcg cgatgaacgc atacagcgct ttgcgatccg
     2941 aaaaatcgca ggcatacccg cggaacttgc gtcccacttc gacgacgcgt cgctccactt
     3001 caccgcccgg ctccagcgtc gcgctgacac caatcacatc cgcgcccgct tcggctagcg
     3061 cgatcgcgat cgccttgccg atgccgcgct tggcgccggt gaccagcgcg gttttccctt
     3121 cgatgcggaa ctgctccagg atcatgcgtt cgctcctcgt gtgctgagcc ggcggcgcat
     3181 ataacggata ctcgaacgga agtcaccctc gccccgcggc acgcgatgcc gcgcgcgatg
     3241 aaccggccca ccctcgcatc ttgcgtccat cgctaccgct tctttatcgc tcgattccca
     3301 cagcgcgacc cttcagtaat cgcttcgcga ttgcagcaac tttggcttct tggagcttgc
     3361 actcccatag atggaacact cgccatccca gcccgcgtag cgcgtgagca ttccgcctgt
     3421 cgcgcgcacg gttgcgggcg attttcgctt tccagtactt ccgcctcgtt cgtgggacgc
     3481 ggcagccgcg cttacaatga tggccatgcc agaaacatcc gtgaacgaac aaggcgacgc
     3541 gcagcgaagg aatgacaatg tcgggggcgc cgggcaatcg acgctcgtta agcttaatca
     3601 ctcgtcggcc cagtcgtggc agcagcaacg cgagagcgtt cacctctgcg gttgtcccca
     3661 ccgagggaac agcgcgcatg atggctgatc gaatcgcagg ggtgaagaca tcagacatga
     3721 acgctagctc gctccgccaa tttcgagagc agtgattcgt ccagccaacg gatgacgggg
     3781 acgcagacgc catccgcaaa cccaaacatc gcctgcgtgt caggaacgcg aattgggaag
     3841 tcacccgcgc cttgcagcct cgcgtattca accgctgaca tccagcgcat tcggacctga
     3901 ccttcatcga taacgatgac aatctgacgc gcgcttccac cctttggtgt gcgaagacaa
     3961 cctgcaagtc catcgaatcg gacttcagcg cgcatgtgcc cgtgtcgccg gcgacgataa
     4021 atcattccga tgtggcggtc accgcgcgaa actagctcat tcacgacact acgatgcagt
     4081 ttgctcatca tctttaggtg cttttgcacc gattcggcct cccaccactc ctggtccttg
     4141 tccagatcca ccaccgccgt cagtggaatt agacattttg gaagtcgtgg cagcgcgtaa
     4201 gttacccagc cggtgggtag gtcgcattcc agcatcgcac ggcgaagccg gtctgggcgc
     4261 aagtccgggc gtgcgctaac gcgatcctcc caggcgctgg ccgccagcca gtctgcgggc
     4321 gtcgtcagca cggccgaatc ttgcggtaaa tagtcccgta gcgcgccgac gataaagagg
     4381 cgcggacgac tctgcggagt aaaatgcttc gcatcaatga ttacagcatc aagaaagtaa
     4441 tccagcgccg ccaactcctt tgaaacgtgg cgaaaatctt cgcctgattt tgaggtgagc
     4501 aggcccggaa cattctcaat tagcaggagg gggggctgcc gctcctcatc acggaggcgc
     4561 tggaggacct tggcgaaccc gaagaatgct gatgattcct cgcctttcaa tcccttgtag
     4621 tagccggcag tggagaggtc ggtgcaagga aacgatgcgg tcgcgagtaa ggttgctggc
     4681 ggaattttcg aagccacctc ggatgtcttc caaacatcct ggacgtgata ataactcgct
     4741 ccgctgtaaa gcgcgcggta catttcgtgc tttttctgct cgatgtcgtt tgcgtaaacg
     4801 cagcgccagc cgccgaattc caagccgcga tgtactaagc caattcccgc gaagaactcg
     4861 gcgaacgttt tggccgcgat tgtttgattc attcttcgca ccagcttacc ctgctgtcgt
     4921 cggtcttgtc tcggggcagt aggatttcaa cagcttccca acaacgatga acttcagcga
     4981 cgatttactg ccgctcctag acgaatggta cgagcggctt gcgatgcttc gcggaagcac
     5041 ccagcgcggg caaggcacgt actttggagc tctggtcatt ctgcaaaacc tcatcgaggc
     5101 atacaacctc gacccgagcg gacatttcac cgacgagggc gagggcggtc gcatatcggg
     5161 aacgagcgcc gaacgagtcg aaagcattct tgcagccctc gacccgcgca ttcaagttaa
     5221 ccagtatgct ggtcgaacca gtacggggcc gactaaggcg gcccggatac tgctgcaagt
     5281 ttttcaacgt gccggcttgg atcgtgtcaa cggagaggaa cggggacagc tactgcacct
     5341 ctgtcagaaa tacatgtgcg atagactgtg gcagttacag gccggtcgtc cgatttattt
     5401 cgtgctcgat cctgctctca cattgaagca gaaccttgcg cgcctgatag aacagcagcg
     5461 agaatacgag cgcgaccggg cggttgccca gcatctggtt ggagccaaac tcgaactgag
     5521 attccctcgg aagcattatc cggacgtcat cattaatcgc gactctgttg atgcggcgga
     5581 cgcgtctgaa caaaaaccag gcgatttcgt cgtaggggac agcgttttcc acgtcaccat
     5641 tgcgccgacg aaaaagcatt ttgagaagtg cgaacaaaac gtcctcagca atctgcgccc
     5701 tattctgctt gtgcccgaga tccaattggc agcagttgcc ggcgctttgc gactgcagta
     5761 ttcaacgaaa atcgacgtgt tttcgatcga atccttcatc gcgcaaaatg tcgaggaact
     5821 ttcggctttt tcatatggat cgatgcgcag tcgcgtgcga gacctcgtgc agatttacaa
     5881 cgaccggatt gaccaggcgg agcgaggaaa gccttacctg cggatcgatg tctcgcgact
     5941 ctgattgcat ccggcatgcg acggctcggt aaagggagta accttacctc cgcgccggcc
     6001 ggagggatgg
//
LOCUS       DATIMH010000525         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2433580, whole genome shotgun sequence.
ACCESSION   DATIMH010000525 DATIMH010000000
VERSION     DATIMH010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433580"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..496
                     /gene="rpmF"
                     /locus_tag="VLI90_09155"
     CDS             311..496
                     /gene="rpmF"
                     /locus_tag="VLI90_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSV14415.1"
                     /translation="MLPVQKHSKSRKRKRRSHLALKPIHYVRCPQCGSAKLPHAACDN
                     CGYVNPKLALQVEQESA"
     gene            533..1552
                     /gene="plsX"
                     /locus_tag="VLI90_09160"
     CDS             533..1552
                     /gene="plsX"
                     /locus_tag="VLI90_09160"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HSV14416.1"
                     /translation="MRAAVDVMGGDHAPAGILQGCWEAASLLGPEDTILLVGDQKISQ
                     AGLDASGLTEDQKKRYKIVHTTQIVEMDDSPVEAIRGKPDSSIAVMCKLAAKGEADVV
                     ISAGNTGACVAAAQLRMRTLPGISRPGIAVIVPTFYGPVVICDVGANVTPKPSHLEQY
                     AIMAAAYAHAVCGIENPRVGLLSIGEEDAKGNAIVKEARKLMRDEPQINFVGNVEGRD
                     LTKGVVDVIVTDGFVGNVVLKLMEGLTEGLFQQLAHELQETNPGAMDQFKPAMKTIYA
                     KHDWQEYGGAPLLGIGGYCLICHGRSEAKAIKNAIRVGKQLVSSGVNEKIVDTIQHSI
                     PVEEE"
     gene            1625..>2347
                     /locus_tag="VLI90_09165"
     CDS             1625..>2347
                     /locus_tag="VLI90_09165"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HSV14417.1"
                     /translation="MPDYGAIIAGTGSCLPEKRLTNDDLSKMVETNDEWITQRTGIKE
                     RRVVGVGESTASLSTNAAKRALDAAGLTAKDLDLIVVATITPEMGFPSTACFVAAGLG
                     LDSTPAFDLAAACSGFMYGLETATSFIKSGRYRNILVVGAETISRITDYKDRGSCILF
                     GDGAGAAIVQRTNDLKRGIIYSSIHADGHGWELIHHSPGSRHIMDEAMLAERGQFIKL
                     KGREVYKFAVTRFEELIEDAMRK"
BASE COUNT          492 a          697 c          739 g          419 t
ORIGIN      
        1 gccgcatcgc gttgaacctg ctgaagcagg accagagcgt aaaactcggc ctgaagaaca
       61 aacgcctcaa ggccagccga aacctcagct atctcttagc catcgtcacc ggcacatcaa
      121 atgccaaatg actttgccct ggccggtatt ggccggtatt ggccggtcgc ttgacagaac
      181 ctgctcttac ttctaacgta cccctccctg acggtctgct cggcgggttt agcccgcctt
      241 ccttttttgg tggatgttcc gcagacctcg cgatctttag cggtcttact cgttagcggc
      301 aggtgccctc atgcttccgg ttcaaaaaca cagcaagtcg cgcaagcgca agcgtcggtc
      361 ccatctggcg ctcaagccca tccattacgt tcgctgcccg cagtgcggca gcgctaagct
      421 gcctcatgcg gcgtgcgata actgtgggta cgtcaatccc aagctcgccc tgcaggtcga
      481 gcaggaatcg gcataacccc acccacggcg cgcgcggatt gaaggagcaa cattgcgagc
      541 tgcggttgac gtcatgggcg gagaccacgc cccggcgggt atcctgcagg ggtgttggga
      601 agccgcgtcc ctgcttggac cggaagacac catcctgctg gtcggcgatc agaagatcag
      661 ccaggccggc ctcgacgcca gcggtctcac cgaagatcaa aagaagcgat acaaaatcgt
      721 ccacacgacg cagatcgtcg agatggatga ctcgcccgtc gaggcgattc gcgggaagcc
      781 ggacagttcg atcgcggtga tgtgcaagct ggccgcgaaa ggcgaagcgg atgtcgtcat
      841 cagcgccggg aacaccggcg cgtgcgtggc cgcggcgcag ctgcggatgc ggacgctgcc
      901 cggcatcagc cggccgggga ttgcggtgat cgtgccgacg ttttacgggc ccgtcgtcat
      961 ttgcgacgtt ggcgcgaacg tcacccctaa gcccagccac ctggaacagt atgcgatcat
     1021 ggccgcggcg tacgcgcacg cggtgtgcgg gatcgaaaac ccgcgcgtgg gccttttgtc
     1081 gatcggcgaa gaagacgcca agggcaacgc gattgtgaag gaagcccgta aattgatgcg
     1141 ggatgaaccg cagatcaact tcgtcggcaa cgtcgaaggc cgcgacctga ccaagggcgt
     1201 ggtggatgtg attgtgaccg atggcttcgt cggcaacgtc gtgctgaagc tgatggaagg
     1261 tctgaccgaa ggtctgttcc aacagctggc gcacgagctg caggaaacca atcccggcgc
     1321 gatggaccag ttcaagccgg cgatgaagac gatttacgcc aagcacgatt ggcaggaata
     1381 cggcggcgcg ccgctgctcg gcatcggcgg ttattgcctg atctgccacg gccgcagtga
     1441 agcgaaagcc atcaaaaacg ccatccgcgt cggcaagcag ttggtcagca gcggtgtgaa
     1501 tgaaaagatc gtcgatacga tccagcattc gattccggtg gaagaggaat aaaaagtagc
     1561 acgggcgtct cgcccgtgtc tgagcacctt ggcacgggcg agacgcccgt gctacttaac
     1621 accaatgcct gattacggag ccatcatcgc cgggactggc agttgcctgc ccgagaagcg
     1681 gttgaccaac gacgatctgt cgaagatggt cgaaaccaac gacgagtgga tcacgcagcg
     1741 caccggcatc aaggagcggc gggtcgtcgg cgttggtgaa agcaccgcca gcctgtccac
     1801 caacgcggcc aagcgcgcgc tcgacgcggc ggggctgacc gctaaagatc tcgatctcat
     1861 tgtcgttgct acgatcacgc cggaaatggg cttcccatcg acggcatgtt tcgtcgccgc
     1921 ggggctgggg ctcgattcga cgccggcgtt cgatctcgcc gccgcgtgca gcgggtttat
     1981 gtacggactg gagacggcaa cgtcgttcat caagtccggc cggtaccgaa atattctggt
     2041 cgtcggcgct gagacgatta gccggatcac cgactacaaa gaccgcggca gctgcatcct
     2101 gtttggcgac ggcgccgggg ctgccatcgt gcagcgcacg aacgatctca agcgcggcat
     2161 catctattcc agcattcatg ccgatggcca tggttgggaa ctgatccacc attcgcccgg
     2221 cagccggcac atcatggatg aggcgatgct ggccgagcgc gggcagttca tcaagttgaa
     2281 gggccgcgag gtgtacaagt tcgccgtgac gcgcttcgag gaactgatcg aagacgcgat
     2341 gcgcaag
//
LOCUS       DATIMH010000526         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2433581, whole genome shotgun sequence.
ACCESSION   DATIMH010000526 DATIMH010000000
VERSION     DATIMH010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433581"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1206)
                     /locus_tag="VLI90_09170"
     CDS             complement(55..1206)
                     /locus_tag="VLI90_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14418.1"
                     /translation="MHVLGQEIHAAPGVALIVAAGVTLYIASRAAADALVGSRPRAPG
                     LMALGHWLPIAAVALAAMVMRRTEIAVGVVFATSVASLTLAIGAVTFLAPPATPAVAR
                     RTWPMILPAAMLVFLAGFHGALTLQHAIFLFIEGIAVCMLWNDRRGLATVVDDASSAP
                     APPPGRGRFSVLNFSQLILAAALAAIGAWAGVHGADIGSSATRVNYTAIDVASAGLLA
                     TTMLGPLLVLPMLGAGVDLAHRGQSWVAVTSQIAVVLLNLCLLLPLVVIEGQYHPLRD
                     AVNSAKTALAPPRPVVVATALTTRPATAPAADEEDGDAATTAPVAEAGVSLPLAVWRV
                     DVVVLIALGMFLLPIALGRWSLTKYEGLGLIGAYIAYLLLAMSLGVRLI"
     gene            complement(1422..2774)
                     /locus_tag="VLI90_09175"
     CDS             complement(1422..2774)
                     /locus_tag="VLI90_09175"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HSV14419.1"
                     /translation="MDVTVIGCGYVGLVTGTCLASIGHQVRGVEKDVKKLKTLMDGHC
                     PIFEPGLQELMQENYASGQLQFTDNVKAAVKDAEVVFLCVGTPPKPDGTVDMSYLEGA
                     GKEVCDALAENNNDYMVTIIVKSTVPCGTNRKLYNFLKQQTKAHVQVVSNPEFLKEGT
                     AVQDCLNPDRIVIGGESPEAFRMMRRLYDPLVKREENFLTMNWESAELTKYAANSMLA
                     ARISFMNEMTILCEAYNADIEDIRKGIGSDQRIGPAFLRAGCGYGGSCFPKDVAAMEH
                     ISKAVGHDNLFVSAIQTVNKNQKKRFAEKIIQKLGRPIQGATIAVWGLAFKADTDDIR
                     ESAAIDVIRYLLDQGAVVKAHDYKAMENMKATFKDEVEWCIDPVTAAAGADAVALCTD
                     WPQYTTLPFRKIAATMNAPIIFDGRNCLHRDVMRETGFQYYPMGRPAVENTLRLKHKV
                     "
BASE COUNT          539 a          974 c          949 g          539 t
ORIGIN      
        1 ccgagcccgg cgtgagaagg tctgatcacg tattcacgga ttcatcccct tacgttagat
       61 caaccgcacc cccagcgaca tcgccagcag caggtatgcg atgtacgcgc cgatcaagcc
      121 cagtccttca tacttggtca gcgaccagcg gcccagcgcg atcggcagca ggaacatgcc
      181 cagggcgatc agcacgacca cgtccacgcg ccacaccgcc agcggcaggc tcacgccggc
      241 ttccgcgacg ggcgcggtgg tcgcggcgtc cccatcttcc tcatcggccg ccggcgcggt
      301 ggccggccgt gtggtcagcg ctgtcgcgac gaccaccggg cgcggcggcg ccagcgccgt
      361 cttcgccgaa ttcaccgcgt cgcgcagcgg gtggtattgg ccttcgatca cgaccagcgg
      421 cagcagcagg cagaggttca gcagcacgac cgcgatctgc gatgtgacgg cgacccagga
      481 ctggccgcgg tgggccagat cgacgcccgc gccgagcatc ggcaggacca gcagcgggcc
      541 gagcatcgtc gtcgccagca gtcccgcgga ggcgacgtcg atcgcggtgt agttcacgcg
      601 cgtcgccgag ctgccgatgt cggcgccgtg tacgcccgcc cacgcgccga tcgccgccaa
      661 agccgcggcc aggatcagct gggaaaagtt gagcactgaa aatcgcccgc gcccgggcgg
      721 cggcgctggc gcggacgacg catcatcgac gaccgtggcc aacccgcggc ggtcgttcca
      781 cagcatgcat accgcgatgc cttcgatgaa caggaagatc gcgtgctgca gcgtgagcgc
      841 gccgtggaag ccggcaagaa aaaccagcat cgccgccggc aggatcatcg gccaggtccg
      901 ccgggcgacg gccggcgttg ccggtggggc gaggaatgtc accgcgccga tcgccagcgt
      961 caggctggca acgctcgttg cgaacaccac gccgacagcg atttcggttc gccgcatcac
     1021 catcgccgcc agcgcgacgg cggcgattgg caaccaatgt cccagcgcca tgagccccgg
     1081 cgctcgcggg cgcgagccca cgagcgcatc cgccgccgcg cgcgatgcga tataaagcgt
     1141 cacgcccgcc gccacgatca gcgcgacgcc cggcgccgcg tgaatttcct gccccagcac
     1201 atgcatgcgg gcagcgatgg tggcgggaac gagggggaat gtccagaacg gcagggtgtg
     1261 tggaacgcgc caccaaagcc cacggctatg ccgtgtgacg gtttttgagg atcggtccca
     1321 cggcgcagcc atgggctttg gtcgcggatc gataaaaaga gcgggacccg ccgaattctc
     1381 ggcgggtccc gttgtttcaa cacctgatcg ggtgaatgcg ctcacacctt atgcttcaac
     1441 cgcagcgtgt tctccaccgc tggccggccc attgggtaat actggaaccc ggtctcgcgc
     1501 atcacgtcgc ggtgcaggca gtttcggcca tcgaagatga tcggcgcgtt catcgtcgcc
     1561 gcgatcttgc ggaacggcag cgtcgtgtac tgcggccaat cggtgcacag cgccaccgcg
     1621 tccgcgccgg cggcggcggt cacggggtcg atgcaccatt ccacctcgtc cttgaaggtg
     1681 gccttcatgt tctccatcgc cttgtagtcg tgcgccttca cgaccgcgcc ctggtcgagc
     1741 agataacgaa tgacgtcgat ggcggccgac tcgcggatgt catccgtgtc ggccttgaac
     1801 gccaggcccc acacggcgat cgtcgcgccc tggatcggcc ggccaagctt ctgaatgatc
     1861 ttttcggcga agcgcttctt ctggttcttg ttgaccgtct gaatcgcgct gacgaacagg
     1921 ttgtcatgac cgacggcttt ggagatgtgc tccatggcgg ccacatcctt ggggaagcag
     1981 ctgccgccgt acccgcaacc ggcgcgaagg aacgcggggc cgatgcgctg gtcggaacca
     2041 atgcctttgc ggatgtcttc gatgtcggcg ttgtacgcct cgcacaggat cgtcatctcg
     2101 ttcatgaacg agatgcgcgc cgccagcatc gaattggcgg cgtacttggt cagctccgcg
     2161 ctttcccagt tcatggtcag gaagttttcc tcgcgcttga cgagcgggtc gtacaagcgg
     2221 cgcatcatgc gaaatgcttc gggcgattcg ccgccgatca cgatgcggtc ggggttcagg
     2281 cagtcctgaa cggcggtgcc ttctttcagg aactcgggat tgctgaccac ttgcacgtgc
     2341 gccttggtct gctgcttgag gaagttgtac agcttgcggt tggtgccgca ggggacggtc
     2401 gacttcacga tgatcgtcac catgtagtca ttattgttct cggccagcgc atcgcagact
     2461 tccttgcccg cgccttcgag gtaactcatg tcgacggtgc cgtccggctt gggcggggtg
     2521 ccgacgcaca ggaagacgac ttctgcgtcc ttcaccgccg ccttcacgtt gtcggtgaat
     2581 tgcagttggc ccgacgcgta gttctcctgc atcagctcct gcaagcctgg ctcgaagatc
     2641 gggcagtgcc cgtccatcag cgtcttgagc ttcttaacgt ccttctcgac gccgcgaacc
     2701 tgatggccaa tgctggccag gcacgttccg gtgaccagcc ccacataccc gcatccgatg
     2761 acggttacgt ccatgacgaa acacctgtgt taggaagaca accaatcgcg cgataacgcc
     2821 caataaagta tacgaaccgc gaggcggcgc ggctcagtaa agatcggaca gattcgctct
     2881 ttgcttgtta gttggcaagc gagtttttgg gttctaaggc aaaaaggaac gcgcgggcaa
     2941 taccaaccta gtcggttgag agcaacgttc gcaatcgaca cggctatgca tcgatggcta
     3001 t
//
LOCUS       DATIMH010000527         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_992841, whole genome shotgun sequence.
ACCESSION   DATIMH010000527 DATIMH010000000
VERSION     DATIMH010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992841"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VLI90_09180"
     CDS             complement(<1..758)
                     /locus_tag="VLI90_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSV14420.1"
                     /translation="MIICLGTTPTVQKTLTFDHVEIDAVNRAKDVLAYASGKSVNVAK
                     ILTLLKRDAIALGILGGDSGRFIRRDLDRMHVPHDFLEVEPPTRTCTTVVDQSTKNAT
                     ELVEESAAVPATVYDALFEKLRSLLDAGGVQALVLSGTLTPGAPQDFYARCVAAGVAA
                     NVTVVLDAKGEPLRRALPMRPTIVKPNRGELEDTVGEKIDSDDAMRDAIAKLIDAGPR
                     WCVVTAGAGNIIASGGASFWRITSPKVPAVSPIGS"
     gene            complement(832..1302)
                     /locus_tag="VLI90_09185"
     CDS             complement(832..1302)
                     /locus_tag="VLI90_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV14421.1"
                     /translation="MDIRPATADDVPAVLPMVAKLAALHEAWDAAKFGYRDRPEEMYR
                     GWLRSRSTDARSVFLVAEREGRVVGFLVGTVETEIPIYHLKEFGFIHDIWVEPEYRNE
                     GVARQLVMLAIERFRAIGVKQVRGDTAAANEPARGLLRSCGMRPSTIEMLIELE"
     gene            complement(1493..2491)
                     /locus_tag="VLI90_09190"
     CDS             complement(1493..2491)
                     /locus_tag="VLI90_09190"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HSV14422.1"
                     /translation="MFIVDAHLDLSYNALRGRDVLRPAREQSPDEEGIPTVGLPDLKA
                     GGVGLVCATVFTMPSLDGKPGYHNADEAYAEGQRHLEWYRRQIDAGVMRFVRNRAEIE
                     AIRDDASSHSAILLLEGADPLRTPADVAAWHATGLRAVGLAWRQTRHAGGTGAPGPLT
                     SDGIALVKELDRFGIIHDTSHLAEQSFWQLLDLTGGPIIASHSNCRAIVPTDRQLSDE
                     MIRAIAARGGVIGINFFDRFLLPPDQYGKRRATLGDVATHVKHICDVTGSAQHVALGT
                     DMDGGLGRNEIPIEIETSGDLPKIADALSDAGFNDLDVEGIAGANWLAYFRKYLPT"
     gene            complement(2508..>2954)
                     /locus_tag="VLI90_09195"
     CDS             complement(2508..>2954)
                     /locus_tag="VLI90_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14423.1"
                     /translation="DDPTILFISLHQHPSTCYPGSGFEWEVGDGPGRGYTMNIPFDPA
                     SGDQDYLRVIKSRVVPKLEAFGPQALLISAGFDGHRDDPLAQMELSEDGFEQMTSMLI
                     ETADRLCDGRVVSVLEGGYNLRALGRSVVRHLVALGAQTSTTGRPA"
BASE COUNT          512 a          924 c          949 g          569 t
ORIGIN      
        1 gacccgatcg ggctaaccgc cggtaccttc gggctggtga tgcgccagaa tgatgcgccg
       61 ccggacgcga tgatgttgcc cgcgccggcg gtgacgacgc accagcgcgg gccagcgtcg
      121 atcaatttag caatcgcatc ccgcatcgcg tcgtccgaat cgatcttctc gcccacggtg
      181 tcttccaact ccccgcggtt gggcttcacg atcgtcggcc gcatcggcag cgcgcgacgc
      241 agcggttcgc ccttggcgtc gaggacgacg gtgacattcg ccgcaacgcc cgcggccaca
      301 catcgcgcat aaaaatcctg cggcgcaccc ggcgtcagcg tgccggacaa gaccagcgcc
      361 tgcacaccac cggcgtctaa caatgatcgc aacttctcaa acagcgcgtc atacaccgtc
      421 gcgggaacgg cggcagattc ttcgaccagc tccgtcgcgt tcttcgtcga ttgatcgacg
      481 acggtcgtac acgtgcgcgt cggcggctcc acttccagga agtcgtgcgg cacgtgcatg
      541 cggtccagat cgcggcggat gaatcggccg ctgtcaccgc cgaggatgcc gagcgcgatc
      601 gcgtcgcgct tcaggagcgt caggatcttc gcgacgttga ccgacttgcc cgatgcgtac
      661 gccagcacgt ccttggcgcg attcaccgcg tcgatctcga cgtgatcgaa cgtgagcgtc
      721 ttctgaacgg tcggggttgt gccgaggcag atgatcatgc gtgattacgt gattacgtga
      781 ttacgtgaag ggcttcgtcg tcgttgcgtg ttcacttcat tacatgctcg attactcaag
      841 ctcgatcagc atctcgatcg tactcggccg catgccgcag gagcggagca gcccgcgggc
      901 cggttcgttc gcggcggcgg tgtcgccgcg gacttgcttg acgccgatcg cgcggaagcg
      961 ttcgatcgcg agcatcacca gctgccgggc gacgccttca ttgcggtatt ccggctcgac
     1021 ccagatgtcg tggatgaagc cgaactcctt caggtggtaa atcgggatct ccgtctcgac
     1081 ggtgccgacg agaaacccga caacgcgacc ttcgcgctcg gccaccagga agacgctgcg
     1141 cgcgtccgtc gagcggctgc gcagccagcc gcggtacatt tcctcggggc gatctcgata
     1201 tccgaacttc gccgcgtccc acgcttcatg caacgccgcc agcttcgcca ccatcggcag
     1261 cacagccggc acgtcgtcag cggtggcggg gcggatgtcc atcgtgtcgg attatcacaa
     1321 tcttcgcgga agcgtaaatg cgctgtgcat gtcgaagtta atttgaacgc agagacgcag
     1381 agacgcggag gccgcagagg catacgccga ggaataattg ctagtgtttg ctttgcggcc
     1441 tctgcgtctc tgcgttcaag attaatcttt cttccttctg cgcgcttgaa agtcatgttg
     1501 ggaggtactt gcgaaagtag gcgagccagt tggccccggc gattccctcg acatcgagat
     1561 cattaaatcc ggcgtccgag agcgcgtcgg cgattttcgg tagatccccg cttgtctcaa
     1621 tttcaatcgg aatctcgttt cgacccaggc cgccgtccat gtcggtgccg agggcgacgt
     1681 gttgcgcgct gccggtgaca tcgcagatgt gcttcacgtg cgtcgcgacg tcgccgagcg
     1741 ttgcccggcg cttgccgtat tgatcgggcg gcagcaggaa gcggtcgaag aagttgatgc
     1801 cgatgacgcc gccgcgggcg gcaatggcgc ggatcatctc gtccgacagt tggcgatccg
     1861 tcggcacgat ggcgcggcag ttggaatgcg atgcgatgat cgggccaccg gtcagatcga
     1921 gcagttgcca gaaggattgt tcggccaggt gtgaggtgtc atggatgatg ccgaagcggt
     1981 cgagttcttt gaccagggct atgccgtcgc tcgtcaacgg ccccggcgcg ccggtgccgc
     2041 cggcgtggcg cgtttgccgc caggcgaggc cgacggcgcg tagtccggtc gcgtgccacg
     2101 cggcaacgtc ggcgggggtg cggagggggt cggcgccttc gagcagcagg atcgcggagt
     2161 gtgatgaagc atcatcgcga atcgcttcga tctccgctcg gtttcgtacg aagcgcataa
     2221 cgccggcgtc gatctgccga cgataccatt caagatgccg ctggccttcg gcgtacgctt
     2281 cgtccgcgtt gtgatatccg ggcttgccat cgagcgacgg cattgtgaag acggttgcgc
     2341 agaccaaacc aacgccgccg gctttcagat ccggcagacc gacggtggga atgccttcct
     2401 cgtccggtga ttgctcgcgc gcggggcgca gtacatcgcg cccgcgaagc gcgttgtaag
     2461 acaggtccag gtgtgcatcg acgataaaca tgaaactggc cggacgctta agcgggtcgg
     2521 cctgtggttg aagtctgggc gccgagcgcg acgaggtgcc gcacgacgct gcgccccagc
     2581 gcgcgcaggt tgtacccgcc ttcgagtacg ctcaccacgc ggccgtcgca caatcgatct
     2641 gccgtctcga tcagcatgct cgtcatttgc tcaaatccgt cttcgctcaa ttccatctgc
     2701 gccagcggat cgtcgcgatg cccatcgaac cccgcggaaa tcagcagcgc ctgcgggccg
     2761 aacgcctcca gcttcggcac gacccggctc ttgatcacgc gcaggtaatc ctgatcgccg
     2821 gaggcgggat cgaacgggat gttcatcgtg tacccgcgcc cggggccgtc gccgacttcc
     2881 cactcgaaac cgctgccggg atagcaggta ctggggtgct ggtgcaggct gatgaacagg
     2941 atggtcggat cgtc
//
LOCUS       DATIMH010000528         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5038277, whole genome shotgun sequence.
ACCESSION   DATIMH010000528 DATIMH010000000
VERSION     DATIMH010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5038277"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VLI90_09200"
     CDS             complement(<1..533)
                     /locus_tag="VLI90_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14424.1"
                     /translation="MPAQPVAVQQAQGRIFTYPVPQGWRLVNETTNEILIAAPDSTQA
                     IGFTGLERMQVGNSAQFLQRVAQMYGSQNLQILSGNRLPVPNCDAIEAIITYTSGGKP
                     CKAWVRVAVVAQNGFGNGYMLMAIATPEKFDSVVAELKPLCDRIQITDASAAFDRANA
                     AAAARPVYTSSAPSLNHP"
     gene            1020..2066
                     /locus_tag="VLI90_09205"
     CDS             1020..2066
                     /locus_tag="VLI90_09205"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HSV14425.1"
                     /translation="MPQRVTILGDGAMATVCSILLTQGGHTVTMWGAFEESIDRLIQN
                     RENQRLLPGTRVPDAVHLTANDAECFDGCTMILSAVPTQYMRDVWRRLRPHVPVNVPI
                     VSVAKGIENDTLLRPTQIIADVLGGKKSVEAGPTCDWPLAALSGPNIAAELAKYLPAT
                     AVAASMDDAVAQLVQRTFSTQWFRVYTNSDATGVELAGATKNIIAIAAGILDGLAAGN
                     NAKAALVTRGLVEITRLGVAMGAQAETFNGLAGVGDLITTCVSPEGRNRSVGEQIGKG
                     RKLADVLEAMDSVAEGVPTTRSVMQLAGNYRVEMPIAAAVHAVLFEGKDAIYALTDLM
                     TRDPKPERADLSIG"
     gene            2278..>3288
                     /locus_tag="VLI90_09210"
     CDS             2278..>3288
                     /locus_tag="VLI90_09210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV14426.1"
                     /translation="MPTTRRSFIKGAATIAGAATLTKNFAFAQSTQPAAEGELPLQTP
                     ASTRQGDMLYRTLGRTGEKVSLIGLGGFHISMQKTDDQSIAIIRRAIDSGITFMDNCW
                     DYRDGLSERRMGLALRDGYRQKVFLMTKIDGRTKEEAAKQIDQSLQRLQTDHVDLLQH
                     HEVLRMEDPDRIFAKGGSMEAVLDAKKAGKLRYIGFTGHKDPVVHLRMLDVAQQHGFR
                     FDTVQMPLNVMDAHFRSFGRMVLPELVRQEIGVLGMKSMGDQFILKSNVVKPIECLHY
                     AMTLPTSVVITGIDSMPILEQALEAVRTFKPMSRDEVAALLDRTRSVAMAGEFEPFKT
                     SAH"
BASE COUNT          624 a          939 c         1119 g          606 t
ORIGIN      
        1 ggatggttca ggctcggcgc gctggacgtg tagacaggcc gagcggcggc ggcggcgttg
       61 gcgcggtcga aggcggcgct ggcgtcggtg atctgaatgc gatcgcacag cggcttgagt
      121 tcggcgacga cgctgtcgaa cttttccggt gtggcaatcg ccatcagcat gtaaccattg
      181 ccaaacccgt tctgcgcgac gacggcgacg cggacccacg ctttgcaggg cttgccgccg
      241 gaggtgtagg tgatgatggc ttcaatcgca tcgcagttgg ggaccggcag gcggttgccg
      301 ctgaggatct gcaggttttg cgagccatac atctgggcga cgcgctggag gaactgcgcg
      361 gagttaccga cctgcatgcg ctccaggccg gtgaagccga tggcttgcgt ggagtcgggg
      421 gcggcgatga ggatttcgtt ggtggtttcg ttaacaagcc gccagccctg ggggacgggg
      481 taggtgaaga tgcggccctg ggcctgctgg acggccaccg gctgagctgg catgggcgct
      541 gcctgctggg caggggcggt gttattggca aaggtctgag cggcggcgac ggaagcagtt
      601 aacgtcgcgg cggcatatgc tgtaacaatc tttcggaaca tggtttaccc tttgcctgga
      661 gttctttaat ttggagacca caacgaagtg gcgttctctg gcaatgggtt cgaagaaaag
      721 acggtagcgc tgcgggggtg ggaagaatgg cggtgggtta taagcgttgc gtagccgcgt
      781 ggctcttccc gcctctcccg gtactccggg agagggctgg ggtgagggtg attttagata
      841 tcgatagccg ttggtgctcg aaatcaccct caccctaacc ctctcccgga gtaccgggcg
      901 aggggaccag agaagcgccg gaccacaaag ttcagttaca cccacgcggc gtgcccgctt
      961 ccgcgtgagc ttccgcccaa ctttgccatt tccaactttc cacttaccat cgctccgcga
     1021 tgccccagcg cgtgacaatc ctcggcgacg gcgcgatggc caccgtctgt tcgatcctgc
     1081 tcacccaggg cgggcacact gtgacgatgt ggggggcgtt tgaagagtcg atcgaccgac
     1141 tgatccagaa ccgcgagaac cagcggctgc tgccgggcac gcgcgtgccg gacgcggtgc
     1201 atctgaccgc gaacgacgcc gagtgcttcg acggctgcac gatgatcctg tcggcggtgc
     1261 cgacgcagta catgcgcgac gtgtggcggc gcctgcggcc gcacgtgccg gtgaatgtgc
     1321 cgatcgtttc tgtggcgaag gggatcgaga acgatacgct gctgcggccg acgcagatca
     1381 tcgcggatgt gttgggcgga aagaaatcgg tagaagccgg tccgacttgc gattggccgc
     1441 tggcggccct gtccggtccg aatattgcgg cggagttggc caaatatctc ccggcaacgg
     1501 cggtggcggc gtcgatggat gatgcggtcg cacagctcgt gcagcggacg ttcagcacgc
     1561 agtggtttcg cgtttacacc aattcagatg caaccggcgt tgagcttgcc ggcgcgacta
     1621 agaacatcat cgccatcgcg gcggggattt tggacgggct ggcggcggga aacaacgcca
     1681 aggccgccct cgtcacgcgc gggctggtcg agattacgcg actgggcgtg gcgatgggcg
     1741 cgcaggcgga aacattcaac ggccttgccg gcgtcggcga tctcatcacc acctgcgtca
     1801 gccccgaggg ccgcaatcga tccgttggcg agcagatcgg caaaggccgg aagctggcgg
     1861 atgttctcga ggcgatggac agcgtcgccg agggcgtgcc gacgacgcgg tccgtcatgc
     1921 aactggccgg gaattaccga gtcgagatgc ccatcgccgc cgcggtgcac gcggtgctgt
     1981 tcgagggaaa agatgcgatc tacgcgctga cggatttgat gacgcgggat ccgaagccgg
     2041 agcgcgcgga tttgtccatt ggataggaac gatcccgaga cgcgcaatag ccgcggccct
     2101 cgcttgcggt ttcgccaacg gacaagccgc ggttgtcgat ctgcgaaacc gcaagcgact
     2161 gccgagcgat ctaagaaaca ccgcactgtt cctatttttt gccccctcgc gccgaatctg
     2221 atccgcggta agcatgacgg ttcgtctcta cctgcgcgcg aacagaagga gcgacccatg
     2281 ccgaccactc gacgatcatt catcaagggc gcggcgacga ttgccggcgc cgcgacgctt
     2341 acgaaaaatt tcgcattcgc tcaatccacc caacccgccg cggaaggcga gctgccgctg
     2401 cagacgccgg cgtcgacgcg ccagggcgac atgctctacc gcacccttgg ccgcacgggt
     2461 gagaaagtct cgctcatcgg tctcggcgga tttcacatca gcatgcagaa gacggacgac
     2521 cagagcattg ccattattcg acgggcgatc gacagcggca tcaccttcat ggacaactgc
     2581 tgggattatc gtgatggcct gagcgaacgc cggatgggcc tggcgctgcg cgatggatat
     2641 cgtcagaagg ttttcctaat gacgaagatc gacggccgca cgaaagagga ggcggcgaag
     2701 cagatcgatc aatcgctgca gcggctgcag accgatcacg tcgacctgct tcagcaccac
     2761 gaggtgcttc ggatggagga cccggatcga atcttcgcca agggcgggtc gatggaggcg
     2821 gtgctggacg cgaagaaagc gggtaagctt cgctacatcg gcttcaccgg gcacaaggat
     2881 ccggtggtgc acctgcggat gctggacgtg gcgcagcagc atggcttccg cttcgacacc
     2941 gtgcagatgc cgttgaacgt gatggacgcc cattttcgca gctttggccg catggtcctg
     3001 ccggagctgg tgcggcagga gatcggcgtg ctgggcatga aatcgatggg cgatcaattc
     3061 atcttgaaga gcaacgtcgt gaagccgatc gagtgcctgc attatgcgat gacgctgccg
     3121 acgtcagtcg tcatcacggg catcgacagc atgccgatcc tcgagcaggc gctggaagcg
     3181 gtgcggacgt ttaaaccgat gtcgcgcgac gaagtggcgg cgttgctcga tcgcacgaga
     3241 tctgttgcga tggccggaga attcgagccc ttcaagacat cggctcat
//
LOCUS       DATIMH010000529         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2724587, whole genome shotgun sequence.
ACCESSION   DATIMH010000529 DATIMH010000000
VERSION     DATIMH010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724587"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /gene="araD"
                     /locus_tag="VLI90_09215"
     CDS             complement(<1..674)
                     /gene="araD"
                     /locus_tag="VLI90_09215"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF006047.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HSV14427.1"
                     /translation="MGQGKYDALKQRVYRANLALVEAGLVVLTWGNASGVDRDADVMA
                     IKPSGVAYDKLTADDIVVLSLSTGEVVDGRGRPSSDTPTHLHLYRSFLSVGAVVHTHS
                     TCGTSFAQAMREIPCFGTTHADNFYGTIPVTRAMTAAEIETDYELNTGRVIVERFTSV
                     RLNPIDVPGVLVASHAPFAWGKTEDKAVENAIVLEFTAQMALNTLALAPNAQAIPQAL
                     LDKHFLR"
     gene            complement(730..>2195)
                     /locus_tag="VLI90_09220"
     CDS             complement(730..>2195)
                     /locus_tag="VLI90_09220"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HSV14428.1"
                     /translation="TKNGFSRKNVIGIGVDTTGSTPLPVNAAGTALALTPEFQDDPNA
                     MAWLWKDHTAHAEAEEITAAAARMRPQYLAKCGGRYSSEWFWAKVLRASRVAPKVFDA
                     AHTWVEIADWIPAVLAGKTAPAALRRGVCAAGHKAMYNPQWGGFPDDQFLAALDPRLG
                     RVRQSLGDKCYSIAESAGGLSAEWAAKLNLPEGIPVAVGAFDAHLGAVGAGVGPGTLV
                     KIMGTSTCDITVSPLDSSLADVPGLCGIVPESVLPGDFGLEAGQSAVGDIFNWFVNII
                     KPGGDEQATHANLTAAAEKLQPGESGLLSLDWHNGNRTVLVDQRLTGLIVGLTLTSTP
                     AEIYRALIEATAFGARVIMERFEEFGVKVQRVVNCGGIAARSPMAMQIYADVMNRPLA
                     LSRSTQTCALGSAVAAAVVAGKSRGGHESFPSAVKAMTALQDKVFTPQPAAAQVYERL
                     YRLYRNLHDAFGMRDSRQDLYPVMKELLDIRDRVRAK"
BASE COUNT          345 a          736 c          720 g          394 t
ORIGIN      
        1 cgcaggaaat gtttgtcgag cagcgcctgc gggatcgcct gcgcgttcgg agccagcgcc
       61 agcgtgttca gcgccatctg cgcggtgaac tccaagacga tcgcgttctc gaccgccttg
      121 tcttcggtct ttccccacgc gaacggggcg tggctggcca ccagcacgcc ggggacgtct
      181 attgggttca ggcgcacgct ggtgaagcgc tcgacgatga cccggccggt gttcagctcg
      241 taatcggttt cgatctccgc tgccgtcatc gcgcgggtga cggggatcgt gccgtagaaa
      301 ttgtcggcgt gcgtggtgcc gaagcagggg atctcgcgca tcgcctgcgc gaagctggtg
      361 ccgcaggtgc tgtgggtgtg aacgactgcg ccgaccgaca gaaagctgcg gtacaagtgc
      421 aggtgtgtcg gcgtatccga cgacggtcgc ccgcggccat cgacgacttc gccggtggat
      481 agcgacagca cgacgatgtc gtccgcggtg agcttgtcat acgcaacgcc gctgggcttg
      541 atcgccatca cgtcggcgtc gcgatcgacg ccgctggcgt tgccccaggt gagcacgaca
      601 agtcccgcct cgaccagcgc gaggttcgcc cgatacacgc gctgcttcag cgcatcatat
      661 tttccttgac ccatttcgta ctcctgatcg agcaccggtg tcgtcagctg caggacaaca
      721 gcgatgctct cacttcgccc gcacgcggtc gcggatgtcc agcagttcct tcatcaccgg
      781 gtacagatcc tgcctgctgt cgcgcatgcc gaaggcgtcg tgcaggttgc gatacagtcg
      841 atacagccgc tcgtacacct gcgccgccgc gggttgcggg gtgaagactt tgtcctgcag
      901 cgcggtcatc gctttgaccg cgctggggaa cgactcgtgc ccgccgcggg atttaccggc
      961 gacgacggcg gcggcgacgg cggagcccag cgcgcacgtc tgcgtgctgc gcgacaacgc
     1021 cagcgggcgg ttcatcacgt cggcgtaaat ctgcatcgcc atcgggctac gggcggcaat
     1081 cccgccgcag ttgacgacgc gctgcacctt cacgccgaac tcttcgaatc gttccatgat
     1141 gacgcgagcg ccgaacgcgg tggcttcgat cagcgcgcga taaatttcgg ccggcgtgct
     1201 ggtcagcgtg agaccgacga tcagccccgt cagccgctga tcgaccagaa ccgtgcggtt
     1261 gccgttgtgc caatcgagcg acagcaaccc gctttcgccg ggttggagtt tctcggccgc
     1321 cgcggtcagg ttcgcgtgcg tcgcctgctc gtcaccgccg ggtttgatga tgttgacgaa
     1381 ccagttgaag atgtcaccga cggcggattg gccggcctcc agcccgaaat cacccggcag
     1441 gaccgactcg ggcacgatgc cgcagaggcc cggcacgtcg gccagggatg aatcgagcgg
     1501 gctgacggtg atgtcgcagg tgctggtgcc catgatcttc accagcgtgc ccggtcccac
     1561 gccggcgccg accgcgccca ggtgcgcgtc gaacgcgccg actgccaccg ggatgccttc
     1621 gggcaggttc aacttggccg cccactcggc cgacaaacca ccggcgctct cggcgatcga
     1681 atagcatttg tcgcccaggc tctgccgcac tcgccccagc cgcgggtcga gcgcagctaa
     1741 aaattgatca tcgggaaacc caccccattg cgggttgtac atcgccttgt gaccggcggc
     1801 gcagacgccg cggcgcagcg cggccggcgc cgtcttgccc gcgagcaccg cggggatcca
     1861 gtcggcgatt tcgacccagg tgtgtgcggc gtcgaagact ttcggcgcga cgcgggacgc
     1921 gcgcagcacc ttggcccaga accattcgga cgaatatcgc ccaccgcact tggccagata
     1981 ctgcggccgc atgcgcgccg cggcggcggt gatctcctcg gcttctgcgt gcgcggtgtg
     2041 gtctttccac agccacgcca tcgcgttcgg gtcgtcctga aactccggcg tcagcgccag
     2101 ggccgtcccc gccgcgttca ccggcagcgg tgtgctgccg gtcgtgtcca caccgatgcc
     2161 gatcacgttc ttgcgcgaga aaccgttctt ggtgg
//
LOCUS       DATIMH010000530         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3878406, whole genome shotgun sequence.
ACCESSION   DATIMH010000530 DATIMH010000000
VERSION     DATIMH010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3878406"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..2421
                     /locus_tag="VLI90_09225"
     CDS             139..2421
                     /locus_tag="VLI90_09225"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSV14429.1"
                     /translation="MSDQREKRVELILQQLEELPTLPNVAVRVLEVTGRDDSSLKDVV
                     ALLESDQSLTTRVLQLVHRADMGVRGEVTSVERAVSLLGFEAVRSAVLAISVFKTLTE
                     NQTNTGGQFNREDFWKHSLAVACCAESLADKLPVGAVNPSEAFVCGLLHDLGKVALDA
                     ILPKSFNRVVEGADLLRGNIADLERTIIGLDHLVVGKRLAERWQLPIAIREAIWLHGQ
                     LPQALPGSVQSPGLINLITLADLLVREQHLGYSGNYTFAARSVVMDALGLKQSHIDAV
                     MRDLVAHIEPRAKTLGLGQTSSNELYQQALSQANKELSRMGGQLAVKNRRLQVRAQFF
                     DALSAFQGSLRPDAAPQTVLQAIAQTAVEVLTVPAVAAFSLMPGATFAEAVLVNQTGQ
                     VFDTTLIDCPTRPPLPNTGDGPVIAAGDDLEWLISAISPRLAGTARFWICLDADNQTI
                     GGVAWGAAPGESQRLSPQVQELTALSNGWSLALRTAQIREEARNLSEQLAEANRRLHG
                     AQNEILRSKTIITVGEMAAGAAHEMNNPLAVISGRSQLLASQLTDPKLKHAATLIFEQ
                     SHRLSEIITELMDFAKPVPPHTAQIELADLVGRSLHDAKMQSDPSDRTIEVTLTDVPP
                     VLVDETQVSAALTELIANAIQATDPVKGHIDIHAAYDAHSTQVVLMIADNGYGMDEQT
                     LKRAFDPFFSAKPAGRRRGMGLPKALRWVESSGGSIRLESRPNQGTRSLVLLPVSQCQ
                     VADSPDTHDEVELHGRKKAQ"
     gene            2637..>3270
                     /locus_tag="VLI90_09230"
     CDS             2637..>3270
                     /locus_tag="VLI90_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV14430.1"
                     /translation="MLPASTAAVQIKTRHKPRVLIVDDEPALVELINDVVGGLQCKVT
                     VARTIAQAKKLLKGETFELMLTDVNLPDGNGTTLLNVLRRHQPTASAIVITGAPSVDG
                     AITALRSGAVDFLPKPFDNDHLVERVRKALEQQSVLAKQEKRLVRLRDAVKRLGIARR
                     MVSKKVDLLCNDLVSAYGELSKQLDIVRTQENFRKTIEQSKDLEQLLCHTM"
BASE COUNT          652 a         1048 c         1009 g          561 t
ORIGIN      
        1 gattcaaacc acagatgcac acagatgaac acagataaag acagttgcgg tcttatctgt
       61 gttcatctgc gtgcatctgt ggttcggacc tgcccttgaa ttgattcgtg ctcttcgtgc
      121 cttcgtggtt aaatccgtat gtcagaccag cgtgaaaagc gggtggagct gatccttcag
      181 caactggagg agctgccgac gttgccgaac gtcgcggtgc gcgtgctgga agtcacgggg
      241 cgtgacgaca gttcgctgaa agacgtcgtc gcgctgctgg aatcggatca atccctcacc
      301 actcgcgtgc tgcagctcgt ccaccgcgcc gacatgggcg tgcgcggcga agtcacctcc
      361 gtcgagcgcg ccgtttcgct gctgggcttc gaagcggtcc gcagcgctgt gctggccatc
      421 tccgtcttca agacgctgac cgaaaatcaa accaacaccg gcgggcagtt caaccgggaa
      481 gatttctgga agcattcgct ggccgtcgcg tgctgcgcgg agtcgctggc ggacaagctg
      541 cccgtgggcg cggtcaatcc gtccgaagcg ttcgtctgtg ggttgctgca cgacctgggc
      601 aaggtcgccc ttgatgcgat tttgccgaag agcttcaacc gcgttgtcga aggcgcggat
      661 ttgctgcgcg gaaacatcgc cgacctggaa cgaacgatca ttgggctgga tcacctggtc
      721 gtcggcaagc gcctggccga gcgatggcaa ctgccgatcg ccattcgcga ggcgatctgg
      781 ctgcacgggc aactgccgca ggcgctgccg ggcagcgtgc agtcgccggg gttgatcaat
      841 ttaatcacgc tggcggacct gctcgtgcgc gagcagcacc tgggttacag cggcaactac
      901 acgttcgccg cccggtcggt ggtgatggat gcgctggggt tgaagcaatc gcacatcgac
      961 gcggtgatgc gcgacctcgt cgcgcacatc gagccgcgcg ccaagacgct gggcctcggg
     1021 cagaccagca gcaacgagct gtatcaacag gcgctgtcgc aggcgaacaa ggaactgagc
     1081 cgcatgggtg gacagctcgc ggtgaagaat cggcgccttc aagttcgggc gcagttcttc
     1141 gatgcgctca gcgccttcca gggcagcctg cgaccggacg ccgccccgca aacggtgctt
     1201 caagcgatcg cccaaaccgc cgtcgaagtg ctgaccgtcc ccgccgtcgc ggcgttttca
     1261 ttgatgccgg gcgcgacctt cgccgaagcg gtgctcgtta atcaaacggg tcaagtcttc
     1321 gacacgacgc tgatcgactg cccgacgcgc ccgccgttgc cgaacaccgg ggacggcccc
     1381 gtcatcgccg ccggcgatga tctggagtgg ctcatcagcg cgatttcccc gcgcctcgcc
     1441 ggcaccgcgc ggttctggat ctgcctcgat gccgacaatc aaacgatcgg cggcgtcgcc
     1501 tggggcgcgg cccccggtga atcgcagcgc ttgagcccgc aggtgcagga actgaccgcg
     1561 ctgtccaacg gctggtcgct cgcgctgcgc accgcgcaaa tccgtgaaga ggccagaaat
     1621 ctttccgagc aactggccga agccaaccgc cggctgcacg gcgcgcaaaa cgaaatcctg
     1681 cgcagcaaga cgatcatcac cgtcggtgaa atggccgccg gcgccgccca tgagatgaat
     1741 aacccgttgg cggtcatctc gggtcggtcg caattgctgg catcgcaatt aacggacccg
     1801 aagctgaagc acgcggccac gctcattttc gagcagagcc atcgcctcag cgagatcatc
     1861 acggaattga tggacttcgc gaagcccgtg ccgccgcaca cagcgcagat cgagttagcg
     1921 gacctcgtgg gacggtcatt acacgacgcg aagatgcagt ccgatccatc cgatagaact
     1981 attgaagtga ccttgacgga tgtgccgcct gtgctggtcg atgagacgca agtgtcggcg
     2041 gcactcacgg agctgatcgc caatgcgatc caagcgaccg atccagtgaa ggggcacatc
     2101 gacattcatg cggcgtacga cgcccattcg acccaggtcg tgctgatgat tgccgacaac
     2161 gggtatggga tggacgagca aacgctgaag cgggcgttcg acccgttctt cagcgccaag
     2221 cccgccggtc gtcgccgcgg catgggcctg cccaaggccc tgcgatgggt ggaatcgagc
     2281 ggtggatcaa tccggctgga gagccggccc aaccagggca cccgctcgct cgtgctgctg
     2341 ccggtcagtc agtgccaagt cgctgactcg ccggacacgc acgatgaagt cgagttgcac
     2401 ggacgcaaga aggcgcagtg attttcctgt cccctcgccc ggtacaccgg gagagggtta
     2461 gggtcagggt gattttgacg aacgatgaac gcggaacgat gaacgtgcga atcagcgcat
     2521 tgctcatcgt tcatcgctca tccttcatcg ctccggtaaa tcaccctcgc cccgaccctc
     2581 tcccggggta ccgggcgagg gggaaatgcc cgtgcaaccg gtgagaggga cagccaatgc
     2641 ttccagccag taccgcagcc gtacagatca agacccggca caagccgcgc gtgctcatcg
     2701 ttgatgacga gccggccctg gtcgagctga tcaacgacgt ggtcggcggc ctgcaatgca
     2761 aggtcaccgt cgcccggacc atcgctcaag cgaaaaaact gctcaaaggc gaaaccttcg
     2821 agctgatgct gaccgacgtg aacctgcccg acggcaacgg caccacgctg ctgaacgtcc
     2881 tccgccggca tcaaccgacc gccagcgcca tcgtcatcac cggcgcgccc agcgtcgatg
     2941 gcgcgatcac cgccctgcgc agcggcgcgg tggacttcct ccccaagccg ttcgacaacg
     3001 accatctcgt cgagcgcgtc cgcaaagcgc tcgaacagca atcagtcctg gccaaacagg
     3061 aaaagcgcct cgttcgcctc cgcgacgccg tcaagcgcct cggcatcgcc cggcgcatgg
     3121 tcagcaagaa ggtcgatctg ctctgcaacg atttggtcag cgcctatggc gagctgtcca
     3181 agcaactcga catcgttcgc acgcaggaaa acttccgcaa gacgatcgag caatccaagg
     3241 acctcgagca gctgctctgc cacacaatgg
//
LOCUS       DATIMH010000531         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_707556, whole genome shotgun sequence.
ACCESSION   DATIMH010000531 DATIMH010000000
VERSION     DATIMH010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707556"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="VLI90_09235"
     CDS             complement(<1..1533)
                     /locus_tag="VLI90_09235"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSV14431.1"
                     /translation="MGSILRPATHRLAALLCALLISTPTLADDAARPPVRVQFASGDT
                     AIAPLRMGQKYFINRKYQLGQLPEELDGLVYTHRNGGRPCEVTIDAPAGATVYLLVDS
                     DKGDNADASGVREMNAKLAESGWTRLEDVKGPARSRPAVYKQTFDAEKRITIAGAGFA
                     GVDVAAKSLVLANLSAQDDKAAGAAAVENVPTAGPTTEVANPQATIKAIELYETESGM
                     MLGQTSEAVLTVTRGDAAKMTNVRFATSVGNEMQMARDDALRFIRINYPNWYADTAEI
                     TFEDKYVKHDGGSIGAAVGTLLLSVIEGFNIDPAAAITGNISANGKVRAIGGVAAKIR
                     GAVASKCSVVAVPNENFNQLIDAMSYGGPSAISDVQIIGISTLADAVAAMRSDRDEKL
                     AKAIALFGEIQQKLKDEALQSPAKKPAAGYIHSKEAQEKLGQVLALAPEHCSAKLLLM
                     VAQDKQPRTLSPAASQYYTFVAVRGMLPVLIERDFTNDKHQVTPAVVRQGLADLKKVR
                     AIA"
     gene            1913..>2205
                     /locus_tag="VLI90_09240"
     CDS             1913..>2205
                     /locus_tag="VLI90_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14432.1"
                     /translation="MTSDETQFGASTSDVAPPPAPPSSRKNKLRRPKRGQRPEDSDGN
                     GHTVARAPQRKPTMRHVALLIESSGSYGRGLLRGIAKYNREHGRWSTYFRPHG"
BASE COUNT          359 a          791 c          647 g          408 t
ORIGIN      
        1 ggcgatcgcg cgcaccttct tcaagtcggc cagtccctgc cggaccaccg ccggtgtgac
       61 ttggtgcttg tcgttggtga aatcgcgctc gatcagcacc ggcagcatgc cgcgcacggc
      121 gacgaacgtg taatactgcg acgccgccgg cgacagcgtt cgcggctgct tgtcctgcgc
      181 caccattagc agcaacttgg ccgagcagtg ctccggcgcc agtgccagca cctgccccag
      241 cttctcctgc gcctctttgc tgtggatata tcccgccgcc ggtttcttcg ccggtgattg
      301 cagcgcctca tccttcagct tctgctggat ctcgccaaac agcgcgatcg ccttcgccag
      361 cttttcatcg cgatcgctgc gcatggccgc gacggcgtcg gccagcgtgg agatgccgat
      421 gatctgcacg tcgctgatcg cgctcggccc gccgtagctc atcgcgtcga tcagctggtt
      481 gaagttctca ttcggcacgg ccacgaccga gcatttgctg gcgacggccc cgcgaatctt
      541 ggcggctacc ccgccgatcg cccgcacctt gccgttggcg ctgatgttcc ccgtgatggc
      601 cgcggccgga tcgatgttga agccttcaat gaccgacaac agcagcgtgc ccaccgccgc
      661 cccgatcgac ccgccgtcgt gcttcacata cttgtcttcg aacgtgatct ccgcggtgtc
      721 ggcgtaccag ttgggatagt tgatgcggat gaatcgcagc gcgtcatcgc gcgccatctg
      781 catctcattg ccgaccgatg tcgcgaagcg gacgttcgtc atcttcgcgg cgtcgccgcg
      841 ggtcaccgtc agcaccgcct cggatgtttg accgagcatc atgccggatt cggtctcgta
      901 gagttcaatc gccttgatgg tcgcctgcgg attggccacc tccgtggtcg gcccggcggt
      961 cggcacgttt tccaccgccg cggcgccggc cgctttgtcg tcctgcgcgc tcaggttcgc
     1021 caacacgagc gacttggccg cgacgtccac accggcgaat cccgcgcccg caatggtgat
     1081 ccgcttctcc gcgtcaaaag tctgcttgta caccgccggg cgcgagcgcg ccggtccctt
     1141 gacatcctcc agccgtgtcc aacccgactc ggccagcttc gcgttcatct cgcgcacgcc
     1201 gctggcgtcg gcgttgtcac ctttgtcgct atcgaccagc agatacaccg tcgcccccgc
     1261 gggggcgtcg atcgtcacct cacacggccg gccgccattg cggtgagtgt agaccaaacc
     1321 gtccaactcc tcgggcaact gtcccagctg gtactttcgg ttgatgaagt atttttgccc
     1381 cattcgaagc ggcgcgatgg cggtatcgcc gctggcgaac tgcacccgca ccggcggccg
     1441 agccgcatcg tcggcgaggg tgggcgtgga tatcagaagc gcgcacaaca gcgcagccag
     1501 ccggtgcgtc gcagggcgca atatcgaccc cataaaacct ccccaacgag ctgccactag
     1561 agatgcgttg agcgatcccg ctccattagt ctaacggtcg gcctcggttg gacaaagctt
     1621 catagcccct tctccctctc cccggacgcc gcggagagga tagggatgag gggcagcgcg
     1681 agaagtcgaa cggttacaga aataccaaat caccccctcg tgtactccaa accgatgcag
     1741 aagacctcca cgccgggttc gcagggcctg ccctgagcct gtcgaagggt actccgacgg
     1801 cccggattct tgctcgacgc gccacggaat ccgggtcgtc gcgagtacgc tccgaacccg
     1861 gcgtgggcgg cagcgccagc gcgccccgag cgtgtatact gtcctccacc ccatgaccag
     1921 cgacgagact caattcggcg cttcgaccag cgacgtcgcg ccgccgcccg cgccgccctc
     1981 ctcgcgcaag aataaattgc gccgcccgaa gcgcggtcag cgcccagaag attctgacgg
     2041 caacggccac accgtcgccc gcgcacctca gcgcaagccg acgatgcgcc acgtcgccct
     2101 cctcatcgaa agctccggtt cctacggccg cgggctgctc cgtgggattg cgaagtacaa
     2161 ccgcgagcac ggccgctggt ccacctactt ccgcccgcac gggtt
//
LOCUS       DATIMH010000532         6343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_707840, whole genome shotgun sequence.
ACCESSION   DATIMH010000532 DATIMH010000000
VERSION     DATIMH010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707840"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2257
                     /locus_tag="VLI90_09245"
     CDS             <1..2257
                     /locus_tag="VLI90_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HSV14433.1"
                     /translation="ESEFDVVVMPHAAHQRRVNLVLVSSVALSFISFWRAAAIVLNDM
                     ASTVYYIGGISEQAIGKAAPWFVLGVMLFAYAVRAVYVESCTMFTRGGVYRVVKEAMG
                     GPLAKLSVSALMFDYVLTGPISAVSAGQYLVGLFGNLLALTPAHFQLTADAHPDLVKL
                     MSMLIAIGITLYFWRINIIGIHESSQKAMRIMQLTTIMGVAVIAWSVLTLFLHPETHH
                     LPPFRPVLTPESQGWLIGVPRIIGMFGIAMAFGHTLLAMSGEESLAQVYREIEAPKVK
                     NLLRAGFVIVVYSLLLTGLVTFFAVMIIPDKERIKTTVVDGTSIVEVSNVVASGGQHV
                     ATLENGSTFTLPATATIVRESGKYTDNLISGLVMHLAGPMPLRLVFQAFVVLVGFLIL
                     SGAVNTSIVGSNGVLNRLAEDGILTPWFLHPQPRFGTTHRLINTVAVLQLITIVASLG
                     DVYTLGEAYAFGVIWSFVFKTLAMVILRFKDKSPRAYEVPLNICIPVRRGAPTEADPL
                     RRVGPGSHDDDRIDLPVGIALIFLVLFGTAIINLFTKKVATIWGIGFTIAFLAVFIIC
                     EQLSRRARRGGHHQHLEQFNEKIAAHPTSIALGLTHPRPLLVAIRNPGSLGALSRVLH
                     ETDTTRQDIVAITCKVLPPLTPGITQQELSVDDIDRAVLTRVVTAAEEAGKPIYPLVL
                     PTNNPLFGIAAAARDLGARQVVLGESQKVSNDSLAEQFALAWGMAMADTAGERPITLR
                     IVGAEDVCIEL"
     gene            complement(2404..3081)
                     /locus_tag="VLI90_09250"
     CDS             complement(2404..3081)
                     /locus_tag="VLI90_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14434.1"
                     /translation="MSQYPSPYSPPPQYPGGYYPAPPDPLAPAKRASLMMFILGPLVI
                     LAGTCLSLFASALNQVTLQPDQQQAIDQVEKTTGVPAHTLFMAAGVVMFVVGLLIVVL
                     GLFVRRGGMGATITSCIVTALILLWLGMNLLGAALHPMGASAGLGICFGVGIFILFGG
                     LLALLVAAARAARGVAAGQQQLQAQYWQYQQNMQSYGGYHAPMTPPPPPPPSAPPSGD
                     QNQNGSA"
     gene            complement(3261..3911)
                     /locus_tag="VLI90_09255"
     CDS             complement(3261..3911)
                     /locus_tag="VLI90_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV14435.1"
                     /translation="MSSTDVPSRSFVSGEYASAQDEEIKSKRIVITGGPGAGKTVISR
                     HLATRYPRRFVLVPEAATQVYDALQTRWDRLDIAGRRDVQRKIYRLQVDQEDRTLAAN
                     PGKVLILDRGTIDGAAYWPEGPADYWRDVGSSLEVELRRYDAVIWMQTSAVLGLYDGD
                     SSNPVRFEHPEAAIRSGELLMQLWAGHPRLRRVEAFVEMAHKFAAVERTLEEVLRK"
     gene            complement(4028..4519)
                     /locus_tag="VLI90_09260"
     CDS             complement(4028..4519)
                     /locus_tag="VLI90_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSV14436.1"
                     /translation="MMNTPADVIAYSLALSRQMLGTFTADLNGADWLARSAQKANCAA
                     WIIGHLVMTERRALTFAGVTDLPPLPAGFEQRFARDEHAAAAGDFGDTSILVPLFDQH
                     RAMTIDYVKRATPEQLNKPLEKPTPRFGTVGEFAAFMAMHTMMHAGQITFIRRSLGRP
                     PIV"
     gene            complement(4738..5406)
                     /gene="lipB"
                     /locus_tag="VLI90_09265"
     CDS             complement(4738..5406)
                     /gene="lipB"
                     /locus_tag="VLI90_09265"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HSV14437.1"
                     /translation="MLVEDLGTLNYREAWTLQERAHDAVLAGEDERILLVEHPPVITF
                     GRRPGVDRHVLASADQLATLGVDVVQSDRGGDITFHGPGQIVAYPIVRLADHHFTVSG
                     YVHALEDAIIATLGDLNLIGSKDPTAVGVWVDDGGVPAKIAAIGVRIRRGVSLHGLAL
                     NVTTDLRYFELIVPCGLQSRPVTSVQKLRGDRAPTIEQTKHLIIQRLIERLTRGGVRS
                     GGAE"
     gene            complement(5621..5836)
                     /locus_tag="VLI90_09270"
     CDS             complement(5621..5836)
                     /locus_tag="VLI90_09270"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSV14438.1"
                     /translation="MRYAVIIEKGPTSYGAYVPDLPGCVAAGETEDEVRQLIREAIEF
                     HIEGLREDGRPVPEPASHIEYVEPKVA"
     gene            complement(5882..6040)
                     /locus_tag="VLI90_09275"
     CDS             complement(5882..6040)
                     /locus_tag="VLI90_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14439.1"
                     /translation="MATKVRDLIRTLEQDGWFIVRTREVIASFITGPRPERLQSRVNR
                     RRTFQRAR"
     gene            complement(6078..>6343)
                     /locus_tag="VLI90_09280"
     CDS             complement(6078..>6343)
                     /locus_tag="VLI90_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14440.1"
                     /translation="PEAHVGSITGDLSGKRGRIQGQDFLPGGMAVVKAQAPLSEVMQY
                     QSQLKSVTGGQGSFAMEFSHYEPVPPQVQAHIVAQYKPKAEEE"
BASE COUNT         1090 a         2037 c         2040 g         1176 t
ORIGIN      
        1 ggagtcggaa ttcgatgtcg ttgtgatgcc ccatgcggcg caccagcggc gggtgaacct
       61 agtgctggtc agcagcgtcg cgctgtcgtt catcagcttc tggcgcgccg ccgccatcgt
      121 gctgaacgac atggccagca cggtgtacta catcggcggc atcagcgagc aggcgatcgg
      181 caaagccgca ccgtggttcg tgctgggcgt gatgctcttc gcatatgcgg tgcgcgcggt
      241 gtatgtcgaa agctgcacga tgttcacccg cggcggggtg taccgcgtgg tgaaggaagc
      301 gatgggcggg ccgctggcga agctgtcggt gtccgcgctg atgttcgact acgtcttgac
      361 cggaccaatc agcgccgtgt cggcggggca atatctcgtc ggcctgttcg gcaatctgct
      421 ggcgctgacg ccagcgcatt tccagctgac ggccgatgcg catccggact tggtgaaact
      481 gatgtcgatg ctgatcgcga tcggcatcac gctgtacttc tggcggatca acatcatcgg
      541 catccacgag tcgagccaga aggcgatgcg catcatgcag ctgacgacga tcatgggcgt
      601 cgcggtgatc gcctggtccg tgctcacgtt attcctgcat cccgagacgc accatctgcc
      661 gccatttcga ccggtgctga cgccggaatc gcagggatgg ttgatcggcg tgccgcggat
      721 catcggcatg ttcggcatcg cgatggcgtt cgggcacacg ctgctggcga tgagcggcga
      781 agaatcgctg gcgcaggtct atcgcgaaat cgaagcgccg aaggtgaaga atctgctgag
      841 ggccggcttc gtcatcgtcg tctattcgct gctgctgacc gggctcgtca cgttctttgc
      901 ggtgatgatc attcccgaca aggagcgcat caagacgacc gtcgtggatg ggacgagcat
      961 cgtcgaagtg agcaacgtcg tcgcctcggg cgggcagcac gttgcgacgc tcgaaaacgg
     1021 atcgaccttc acgctgccgg ccacggcgac catcgtgcgc gaatccggca agtacaccga
     1081 caacctgatc agcggcctgg tcatgcacct ggccgggccg atgccgctgc ggctggtgtt
     1141 tcaggcgttc gtggtgctgg tgggttttct gatcctcagc ggcgccgtca acaccagcat
     1201 cgtcggttcc aacggcgtgc tgaatcgatt ggccgaagac ggcatcctca cgccgtggtt
     1261 cctgcatccg cagccgcgct tcggcacgac ccatcggctg atcaacacgg tggcggtgct
     1321 gcagttgatc accatcgtcg ccagcctcgg cgacgtctac acgctcggcg aagcgtacgc
     1381 gttcggcgtg atctggtcgt tcgtcttcaa gacgctggcg atggtgatcc tgcgcttcaa
     1441 ggacaagtcg ccgcgcgcgt acgaggtgcc gctgaacatt tgtatccccg ttcgtcgcgg
     1501 cgcgccgaca gaggccgacc cgcttaggcg ggtcgggccg ggttcgcatg acgacgatcg
     1561 catcgatctc cccgtcggca tagcgctgat cttcctggtc ctgttcggca ccgccatcat
     1621 caacctgttc accaagaaag tcgcgacgat ctggggcatc ggcttcacca tcgcgttcct
     1681 cgccgtcttc atcatctgcg agcagctgtc gcgccgcgct cgccgcggcg gacatcacca
     1741 gcacctggaa cagttcaacg aaaagatcgc cgcccatccc acgtcgatcg cgctcggcct
     1801 gacgcacccg cggccgttgc tggtggcgat ccgcaacccc ggcagcctcg gcgcgcttag
     1861 ccgggtgctg cacgagacgg acacgacacg gcaggacatc gtggcgatca cgtgcaaggt
     1921 gctgcccccg ctgacgccgg gcatcaccca gcaggaattg agcgtggacg acatcgatcg
     1981 cgcggtgctg acgcgcgtcg tcaccgccgc ggaggaagcg ggcaagccga tttacccact
     2041 ggtgctgccg acgaacaacc ccctgttcgg catcgccgcc gcggcacgcg atctgggcgc
     2101 gcggcaagtg gtgctcgggg aatcgcaaaa ggtctcgaac gattcgctgg ccgagcagtt
     2161 cgcgctggcg tggggcatgg cgatggccga caccgccggt gagcggccga tcacgctgcg
     2221 catcgtcggg gcggaggatg tttgcatcga gctgtagttg gacgcgcgcg ggcgacgcgg
     2281 agactacagt cgctattgat cacgccgggt tcggaggtac tccgacgacc cggatcttgg
     2341 ggcgcgaaga agaatccggg tcgtcggagt acctacgaac ccggcgtggg gggcacctaa
     2401 tctctacgct gatccgttct gattctgatc ccctgacggc ggcgcagaag gaggcggcgg
     2461 tggtggcggc gtcatcggcg cgtgataacc gccatacgac tgcatgtttt gttggtactg
     2521 ccagtattgc gcttgaagtt gctgttgtcc cgccgcgacg ccccgggcgg cacgggcagc
     2581 ggcgacgagc agcgcaagca acccgccgaa caggatgaag atgccgacgc cgaagcagat
     2641 tccgagcccg gcgctggcgc ccatcggatg aagcgctgcg cccagcaagt tcattcccaa
     2701 ccaaagcagg atcagtgccg tcacgatgca cgatgtgatc gtcgccccca tgccgccgcg
     2761 gcggacgaac aatcccagca cgacgatcag gaggccgacc acgaacatca ccacgccggc
     2821 cgccatgaac agcgtgtgag cgggaacgcc cgtcgtcttc tcaacctgat cgatcgcctg
     2881 ctgctggtcg ggctgcagcg tcacctggtt gagggccgaa gcgaacagcg acagacacgt
     2941 gccggccagg atgacgagcg ggccgaggat gaacatcatg aggctcgcgc gcttagccgg
     3001 cgccagcgga tccggcgggg ccgggtaata tccacccgga tactgcggcg gcggcgaata
     3061 aggcgagggg tattgcgaca tcggtggcga ttgtaggtgg atcgtcgcgc gtgcggaaga
     3121 acgcggaacg aacccgacgc cgggctaacc gcttcagcgg ttagcgtgtt cgctcgagtt
     3181 tcatcgtttc aaaatccaga tcaccctcac cctaaccctc tcccggcgta ccgggcgagg
     3241 ggaccggagg acaaacgccg tcacttgcgc agcacttcct ccagcgtccg ctccaccgcg
     3301 gcgaatttat gtgccatctc gacaaacgct tccacccgcc gcaggcgcgg gtggcccgcc
     3361 cacagttgca tcagcaattc gccgctgcgg atggccgctt cggggtgttc gaagcgcacg
     3421 gggttcgagc tgtcgccgtc gtacaggccg agcacggcgc tggtctgcat ccagatgacc
     3481 gcgtcgtagc ggcgcagctc aacctccagc gatgagccga cgtcgcgcca gtaatccgcc
     3541 gggccttcgg gccaataggc ggccccgtcg atcgtgccgc gatcgaggat cagcaccttg
     3601 cccgggttgg ccgcgagcgt gcggtcctcc tggtccacct gcaggcggta aatcttccgc
     3661 tgcacgtcgc gccggccggc gatgtcgagg cggtcccagc gcgtttgcag ggcatcgtac
     3721 acctgcgtcg ccgcctccgg cacgagcacg aaccgccgcg ggtatcgcgt ggcgagatgg
     3781 cgggagatga cggtctttcc ggccccgggg ccgccggtga tgacgatgcg cttcgatttg
     3841 atctcttcat cctgagcgga ggcgtactcc ccggagacga aggatctgga tgggacgtcg
     3901 gtggaactca tgcgggtgat gcggtgatgg cggcgcgctc aatggctggc ggtgcttgcg
     3961 cgagtttgag ttcatggact tcgcggccag atccttcgac tccgagtacg cctgcgctga
     4021 ggatgaacta cacaatcggc ggccggccca gcgaccggcg gatgaatgtg atctgcccgg
     4081 cgtgcatcat cgtgtgcatc gccatgaacg cggcgaattc accgacggtg ccgaaccgcg
     4141 gcgtgggctt ctccagcggt ttgttcagct gctcgggcgt cgcgcgcttc acgtagtcga
     4201 tcgtcatcgc ccgatgctga tcgaacagcg gcacgaggat cgaggtgtcg ccgaaatcac
     4261 ccgcagccgc ggcgtgctcg tcgcgcgcga agcgctgctc aaaccccgcc ggcagcggcg
     4321 gcaggtccgt gacgccggcg aacgtcagcg cgcgccgctc ggtcatgacc aggtgcccga
     4381 tgatccacgc ggcgcagttc gccttttgcg cggatcgcgc cagccaatcc gcgccattaa
     4441 gatcggcggt gaatgtgccg agcatctgcc tcgaaagggc gagggaatac gcaatcacgt
     4501 cagcgggggt gttcatcatg cggctctcct tgtaacgggg cacaatgcta cgcaatcacg
     4561 cgcggcgacg aaagcggcag cattcaggcg atgtacgctt tcgacaccgg tgtctgaggt
     4621 actccgaaga cccgggtacg tgcgctggaa gatgcccgcg tcttcggagt gcctcagaca
     4681 ccggcgtcgg ggctacggcg cgggcgcttc cctcttcatc gctccacaac tcgatcgtca
     4741 ctctgcgccg ccgctccgga cgccaccgcg cgtgagacgt tcgatgagcc gctggatgat
     4801 caggtgcttg gtttgctcga tcgtcggcgc ccgatcccca cgcagtttct gaaccgacgt
     4861 caccggccgg ctctgcaggc cgcaggggac gatcagctcg aaatatcgca ggtcggtcgt
     4921 cacgttcagc gccagcccgt gcaggctgac accgcggcgg atgcgtacgc cgatcgccgc
     4981 gattttcgcc ggcacgccgc cgtcgtccac ccacacgccg acggccgtcg ggtctttgct
     5041 gccgatgaga ttcaagtccc cgagcgttgc gatgatcgca tcttccagcg cgtggacata
     5101 gccgctgacg gtaaagtgat gatcggccag gcggacgatc ggatacgcca cgatctgccc
     5161 gggcccgtga aaggtgatgt ccccgccgcg gtcgctttgg acgacatcga cgccgagcgt
     5221 cgcgagttga tccgctgaag ccaggacatg ccgatccacc cccggccgcc ggccgaacgt
     5281 gatgaccggc gggtgctcga cgagcaggat gcgctcgtcc tcgccggcca gcaccgcgtc
     5341 gtgcgcccgc tcctgcagcg tccacgcctc gcggtaattc aatgtgccca gatcttcaac
     5401 gagcatgttc agaacaataa tagccgcagg cgacagccag cgggggcgct cgatgcaacc
     5461 gcgtcgttct ggaacgatcg cgctggctgt cgtctgcggc tattaatcta atattgttgc
     5521 ctttactctt cctccgcctt cggtttgtac cgcgcgacga tatgcgcctg cacctggggc
     5581 agcgaccacg cgtctccgtc gttcagcgtg ccgaggtcca tcacgcgacc ttcggctcga
     5641 catactcaat gtgagatgcg ggttcgggta cgggacggcc atcttcgcgc aagccttcga
     5701 tgtgaaactc gatcgcctcg cgaatcagtt gtcgaacctc gtcttcggtc tcaccagccg
     5761 cgacgcagcc ggggagatcg ggcacatatg cgccgtagct cgtcgggcct ttctcgatga
     5821 tcactgcata acgcacgtgt gtgtactcct catcgaagtc cggcttgctt caaaatgctg
     5881 ttcaacgtgc ccgctggaac gtccgtcgac gatttacccg cgactgtaac cgttccggcc
     5941 ttggtccggt gatgaaactg gcgatgactt cacgcgttcg cacgatgaac cagccgtctt
     6001 gttccagcgt cctgatcaaa tcgcgaacct tggtggccat ctccctcccg caattctagg
     6061 cactgtgtct cgcgggccta ttcttcctcc gccttcggtt tgtactgcgc gacgatatgc
     6121 gcctgcacct gcggcgggac cggttcgtaa tggctgaact ccatcgcgaa cgacccctgc
     6181 ccgccggtca cgctcttcag ctgtgattga tattgcatca cctcgctgag cggggcttgg
     6241 gctttgacga cggccatgcc gccggggagg aaatcctggc cctgaattcg gccacgtttg
     6301 ccggacagat cgcccgtgat cgaaccgacg tgcgcctcgg gca
//
LOCUS       DATIMH010000533         4861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_417226, whole genome shotgun sequence.
ACCESSION   DATIMH010000533 DATIMH010000000
VERSION     DATIMH010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417226"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VLI90_09285"
     CDS             <1..1035
                     /locus_tag="VLI90_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HSV14441.1"
                     /translation="SARDTFSDRAFTGAGQTFRVTFEPGTEISNASIEFSEPYIFDQP
                     YSNTDEAYYRTYIREHWDESRAGGRVTFGKRINYIWSTSVGVRGEDVNVSSIENYYPL
                     YDRISVINPITGQPNINPATGAAITQPRSPRAPEIIALAGHNVLSSAQFQLRRDTTNH
                     GPLTYKGTDTIFGYEYAGALGGDFHFHKFTLGYDAYQPVFEDLLDRKTVLSFHVESGY
                     ITDNAPFFERFYGGGIGSIRGFSYRGVSPRAGRENDPVGGNFNLVGTLELNYPIYGEN
                     LRGVIFSDAGTVEPDVRIHTIRASVGAGVRLVLPFFGQAPLALDFAVPIFKAPEDETQ
                     FFSFSFGKSF"
     gene            1231..1833
                     /locus_tag="VLI90_09290"
     CDS             1231..1833
                     /locus_tag="VLI90_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSV14442.1"
                     /translation="MNARRVSPLAVALFALAAAVPAMGQAQPNKIGVVNMGKVFNTMQ
                     ETKDIKAHLEADQSTLNSDVQAHKAELENLRQKLANGPKPDSPQYDDLAQQLEQKSVQ
                     YDTEVKLKQIDLARNQSRQLKTIFDKIEGAVGEIAKQKQLDLVITMVKPEFPPNLREL
                     TPEAISQLINSRNVMYVNDKIDVTSDVVTALDAKYKSGGK"
     gene            2025..3068
                     /gene="lpxD"
                     /locus_tag="VLI90_09295"
     CDS             2025..3068
                     /gene="lpxD"
                     /locus_tag="VLI90_09295"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HSV14443.1"
                     /translation="MALSPPIKLADITAALGLPAPGGGDRVVTGIASLADAGPTELAM
                     VTSDAYVKQFAKTAAMAVVANRKVKLPATTAALVFRVDDTDAAIAKVLMLFAPPPERP
                     PAGMHSSAQIDPTATIHASCAIGANVVIGQRSTLGARVLVHPGVVIGDDVTIGDDCQL
                     LPNVVLRQRVTIGNRVSIHAGTVIGTDGFGYRWDGKQHAKVPQIGTVVIEDDVEIGSC
                     CCVDRAKFGATRIGRGTKIDNLVQVAHNVQIGPHSIIIAQTGIAGSARLGANVILGGQ
                     TAVRDHITIGEAANAAARSAIASNVPPKQVVSGMPALPHRQSLREQAALRRLPELLVQ
                     VRKLQEEIKRLKK"
     gene            complement(3183..3905)
                     /locus_tag="VLI90_09300"
     CDS             complement(3183..3905)
                     /locus_tag="VLI90_09300"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HSV14444.1"
                     /translation="MRLDVALTPRDVEPGRLRDRAVVVFDVLRATTTMTAALAAGVAG
                     IRVFESLDAAAAAANGFAEPRVLCGERQCLRPPGFDLGNSPGAFDARQHAGRTVFMST
                     TNGTRAIVAARSAKLLLAGALVNASAVANHLIPAGLDVTLLCAGTDGEPAMEDLLGAG
                     AVIDALAVMRDVELQNDNARVALRMLRCCRGELRPMLADAAGGRNVIAAGLEADIDFA
                     ARLDALDVVGRVLGNPLRVVRG"
     gene            complement(3980..>4861)
                     /locus_tag="VLI90_09305"
     CDS             complement(3980..>4861)
                     /locus_tag="VLI90_09305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSV14445.1"
                     /translation="KSDQALQLILTGFDGKRIDPSGSIDAPSGGDGTVDLRAMYPAVA
                     NPGTYVLFAVPKGGAPADFVGTPIVVSVMADRRPGAPAGPMVIHMEPLRYAVMTTDAG
                     PITIVFYFDVAPNTADSFLRLASQGYFDGLTFHRIIPDFVIQGGDPRGDGTGGPGYNI
                     DAEFNDRPHEEGALSMARNGDPDEGPGVMPRPEFANSAGSQFFICLDYAHTKQLDKRY
                     TVFGKVVDGMDAVRKIAATPLVDAQSGRPVKPPVMQKVEVRAVTPGNNPYSAIIEIGA
                     PTTTQPAGAATAPAAAQ"
BASE COUNT          921 a         1755 c         1414 g          771 t
ORIGIN      
        1 agcgctcgcg acaccttcag cgatcgcgcc ttcaccggcg ccggtcaaac cttccgcgtc
       61 acctttgaac ccggcacgga gatcagcaat gccagcattg aattttccga gccgtacatc
      121 ttcgaccagc cttacagcaa caccgacgag gcatattacc gcacgtacat ccgcgagcac
      181 tgggacgaga gtcgcgccgg tggccgggtc accttcggca agcgcatcaa ctacatctgg
      241 tccacgtcgg tcggcgtgcg cggcgaggac gtgaacgtca gctcgatcga aaactactac
      301 ccgttgtacg atcgaatctc ggtgatcaac ccgatcacgg gtcagcccaa catcaatccc
      361 gccaccggcg ccgccatcac ccagccgcgc agcccgcgcg cgccggagat tatcgcgctt
      421 gccggccaca acgtgctgag cagcgcgcaa ttccagctcc gccgcgacac caccaaccac
      481 ggcccgctca cctacaaggg caccgacacg atcttcggct acgaatacgc cggcgcgctc
      541 ggcggtgatt tccatttcca caagttcacg ctcggttacg acgcgtacca gcccgtcttc
      601 gaagacctgc tcgaccgtaa gaccgtccta agcttccacg tcgaaagcgg ctacatcacg
      661 gacaacgccc cgttcttcga gcgcttctac ggcggcggta tcggcagcat ccgcgggttt
      721 tcctatcgcg gggtcagccc gcgcgccggc cgcgagaacg acccggtcgg tggcaacttc
      781 aatctggtcg gcacgctgga gctgaactat ccgatctacg gcgagaacct gcgcggcgtg
      841 atcttttccg acgccggcac cgttgagccg gacgtgcgga ttcatacaat ccgtgccagc
      901 gtcggcgccg gtgtccggct cgtgctgccg ttcttcggcc aagcgccttt agcgttggac
      961 ttcgccgtgc cgatcttcaa agcgcctgaa gacgaaacgc agttcttcag cttttcgttt
     1021 gggaagagct tttgagatgt ctgctctggt cccctcgccc ggtacgccga gagaggggac
     1081 cggaaagaat tatcggactc gccgcccccc gccgatttgg tcgtgcaacc atagcggcgg
     1141 ccgcgcatgt ctgcgatgat gtcgtcattg tcaacttaag cagtggggag atttccactg
     1201 tgattatcgt tcggagaaag gaaccggatc atgaacgctc gtcgtgtatc gcccctcgcc
     1261 gtcgcactgt tcgcgctcgc cgccgctgtc ccggcgatgg gccaggccca acccaacaag
     1321 atcggtgtgg tcaacatggg caaggtcttc aacaccatgc aggagaccaa ggacatcaag
     1381 gcccacctcg aggccgacca gagcacgctc aattccgacg tgcaggccca caaggccgag
     1441 ctggaaaacc tgcgccagaa gctggccaac ggccccaagc ccgacagccc gcagtatgac
     1501 gatctcgccc agcagctgga gcagaagtcg gtgcagtacg acaccgaagt gaagctgaag
     1561 cagatcgacc tcgcccgcaa ccagagccgg cagctcaaga cgatcttcga caagatcgaa
     1621 ggcgcggtgg gcgaaatcgc caagcagaag cagctggacc tggtgatcac gatggtgaag
     1681 ccggagttcc cgcccaacct gcgcgagctg acgccggagg ccatctcgca gctgatcaac
     1741 tcgcgcaacg tgatgtacgt gaacgacaag atcgacgtca ccagcgacgt ggtgaccgcg
     1801 ctggacgcca aatacaagtc cggcggaaag taatcgggcg caggcccacg ccgccgaagc
     1861 cgctttcatg cgcactcgga gccccggatg tacatccggg gcttctgtta ttgctggatc
     1921 gcattgcgag cgtcgcggat cgttcgtcgc acgcgtagct gtgacgcctg cgtcgccgat
     1981 cgcgcagaag ccgacgcagg cgtcgcatct gcaggttcgg aaacatggcc ctctcacccc
     2041 cgatcaagct cgccgacatc accgccgcgc tgggcttgcc cgcgccgggc ggcggtgacc
     2101 gcgtcgtcac cggcatcgca tcgctggccg acgccggtcc caccgagttg gcgatggtca
     2161 cgtccgatgc ctacgtgaag cagttcgcca agaccgccgc catggcggtc gtcgccaacc
     2221 gcaaggtgaa gctgccggct acaaccgccg ccctggtctt ccgcgtcgat gacaccgacg
     2281 cggccatcgc caaagtgctg atgctcttcg ccccgccacc ggaacgcccg ccggctggta
     2341 tgcattcctc ggcgcagatt gatccgacgg caacgatcca cgcctcctgc gccatcggcg
     2401 ccaacgtcgt catcggccag cgctccacgc tcggcgcgcg tgtgctggtt caccccggcg
     2461 tcgtcatcgg cgacgacgtc acgatcggcg atgactgcca gctcctgccc aacgtcgtcc
     2521 tccgccagcg cgtgacgatc ggaaaccgcg tgtcgatcca cgccggcacc gtcatcggca
     2581 ccgacggctt cggttatcgc tgggacggca agcagcacgc gaaagtgccg cagatcggca
     2641 cggtggtgat cgaggacgac gtggagatcg gcagctgctg ctgcgtcgat cgcgccaagt
     2701 tcggcgcgac gcgcatcggc cgcggcacga agatcgacaa cctggtgcag gtcgcccaca
     2761 acgtgcagat cggcccccac agcatcatca tcgcgcaaac cggcatcgcc ggttcggcgc
     2821 gcctcggcgc caacgtcatc ctcggcggtc aaaccgccgt gcgcgaccac atcacgatcg
     2881 gcgaagccgc caacgccgcc gcccgcagcg cgatcgccag caacgtcccc cccaagcaag
     2941 tcgtcagcgg catgcccgcg ctcccgcacc gccaaagcct gcgggaacaa gcggccctga
     3001 gaaggctgcc cgagctgctg gtacaagtgc gcaagctgca ggaagagatc aagcgattga
     3061 agaagtgaaa acgccgtccc ccacgccggg ttcggagggt actcccgacg aagcgagatt
     3121 cgtcttctac tcacaagacc catccgggtc ctcgcgagta cgctccgaac ccggcctggg
     3181 cgttagccgc gcacaacccg caacgggtta cccagcacgc gccccacgac atccagcgca
     3241 tccaatcgcg ccgcaaaatc aatatccgct tccaaccccg ccgcgatcac gttgcggccg
     3301 cccgcggcat ccgccagcat cggccgcaac tccccgcggc aacatcgcaa catccgcagc
     3361 gcgacccgcg cgttatcgtt ctgcagttcc acatcgcgca tgaccgctag cgcatcgatc
     3421 accgcccccg cccccaacag atcttccatc gccggctcac cgtcggtgcc ggcgcacagc
     3481 agcgtcacgt ccagccccgc cgggatgaga tgattcgcca ccgccgacgc gttcaccagc
     3541 gccccggcca gcagcagctt tgcagaacga gccgcgacga tcgcgcgcgt gccgttggtc
     3601 gttgacatga acaccgttcg ccccgcgtgt tgccgcgcgt cgaacgcgcc cgggctgttg
     3661 cccagatcaa atccaggcgg acgaagacac tgccgctcac cacagagcac gcgcggctcc
     3721 gcgaacccgt tcgccgccgc cgccgcggca tccagcgact cgaacacccg gatccccgcc
     3781 acccccgccg ccagcgccgc cgtcatcgtc gtcgtcgcgc gaagcacgtc gaacacgacc
     3841 accgcgcgat cgcgcagtcg ccccggctcc acatcgcgcg gcgtcaatgc aacatccaaa
     3901 cgcatcttca tacccaccgc gggcgtagtc cccaaaaccg gccgacccgc ttaagcgggt
     3961 cgggccagca cctccatcat cattgggccg ccgccggcgc ggtcgccgcc ccagcaggtt
     4021 gcgtcgtcgt cggcgcgccg atctcgatga tcgcgctata cggattattc cccggcgtga
     4081 ccgcccgcac ttccaccttc tgcatcaccg gtggtttcac cggccggccc gactgggcat
     4141 cgaccagcgg cgtcgccgcg atcttccgca ccgcgtccat cccatcgact actttcccga
     4201 acacggtata tcgcttgtcc aactgcttcg tgtgcgcgta atcgagacag atgaagaact
     4261 ggctgccggc gctgttggcg aactccggtc gcggcatcac gccgggacct tcatccggat
     4321 caccgttgcg ggccatcgac agcgcgccct cttcgtgcgg gcgatcgttg aactcggcat
     4381 cgatgttgta ccccggccca ccggtgccat cgccgcgcgg gtcaccgccc tggatgacaa
     4441 agtcggggat gatgcgatgg aaggtgagcc cgtcgaaata tccctgcgac gcgaggcgca
     4501 aaaagctatc ggccgtgttg ggcgcgacat cgaagtaaaa cacgatggtg atcggcccgg
     4561 catcggtggt catcaccgcg tagcgcagcg gttccatgtg gatcaccatc ggtcccgccg
     4621 gcgcgcccgg ccgccggtcg gccatcacgg aaacgacgat cggcgtgccc acaaaatccg
     4681 ccggcgcccc gcccttgggc accgcaaaca gcacgtatgt ccccgggtta gccaccgctg
     4741 gatacatcgc gcggagatcg accgtgccgt cgccgccgct gggggcgtcg atcgatccgc
     4801 tcgggtcgat ccgcttgccg tcgaacccgg tcaggatcaa ttgcagcgcc tgatccgact
     4861 t
//
LOCUS       DATIMH010000534         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1863379, whole genome shotgun sequence.
ACCESSION   DATIMH010000534 DATIMH010000000
VERSION     DATIMH010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863379"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VLI90_09310"
     CDS             <1..846
                     /locus_tag="VLI90_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSV14446.1"
                     /translation="AGVAAERVHVHNCFLGGGFGRRSMNDELIQAVTVAKAVGKPVKL
                     IWTRETDMQHNRYRPMAVLRARGRIGADGEIEALQFQTVVGSILGSLGWQRGSPGGIE
                     PMAVSGLSDQAYAAKNLLVEAALKNTHIPVMFWRSVGYSQNTFVMESFIDELAHAAGE
                     DPYQFRRKLLANEPEWLRVLDTVAEKSGWGTPLPKGRGRGIAIHMSHDTILAEVAEVA
                     VTPGGEVRVERVVAVADPGWVINPRLIEMQVESGIIYGLTRRCTAPLPSRRAPSSRAI
                     STITA"
     gene            843..1034
                     /locus_tag="VLI90_09315"
     CDS             843..1034
                     /locus_tag="VLI90_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14447.1"
                     /translation="MMLMADAPRIETWLVPSHGKRWGGIGEPGTPPAAPAVTNAIFAA
                     TGVRVRSLPIATADLTSRS"
     gene            1052..1444
                     /locus_tag="VLI90_09320"
     CDS             1052..1444
                     /locus_tag="VLI90_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSV14448.1"
                     /translation="MPAVSLNHYTIMVQDLERTREFYEDVVGLTTGDRPPLAFPGYWL
                     YCGGVPTVHLVGHRPENPQFDGAPETGRLDHIAFAAENLKLMRERLQTRGLEFSERVL
                     PRLDITQLFFKDPDGINIEFNFPAEETV"
     gene            1651..>2529
                     /locus_tag="VLI90_09325"
     CDS             1651..>2529
                     /locus_tag="VLI90_09325"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HSV14449.1"
                     /translation="MQKGRHFHTGIKHSPREKAARVHLGSLEFKKKGVSRFDLRDPYH
                     LAVALTWPQFFATLLVTYLLANVFFASLFWLVPGSVANARPYSFPDAFFFSIETLATV
                     GYGEMYPATLYGHIIAATEIVCGLGFTAILTGLIFVRFSRPRAKLVFATHPVVAMHEG
                     KPTLMLRVGNGRADVLLDAVTKLNVLLIVTTAEGKLFRRAQELRLERAHLPVFPLTWT
                     LMHVMDERSPLYGYDAAQVVAAGAQVFVTLEARDPTLATSVHEIRNYAPEDIRFGMRY
                     VDVITSEEDGTPVADLT"
BASE COUNT          485 a          730 c          825 g          489 t
ORIGIN      
        1 gccggggtgg cggcggagag ggtgcacgtc cacaactgct ttctcggcgg cggctttggc
       61 cggcgatcca tgaacgacga gctgatccag gccgtcacgg tggcgaaggc cgtgggcaaa
      121 ccggtcaagc tgatctggac ccgcgagacc gacatgcagc acaaccgcta tcgcccgatg
      181 gcggtcctgc gtgcgcgtgg acggattggc gcggatggcg agatcgaggc gctgcaattc
      241 cagaccgtgg tgggctcgat cctggggtcg ctggggtggc agcgtggcag cccgggcgga
      301 atcgagccga tggcggtttc gggattgtcc gaccaagcct atgcggcgaa gaacctgctg
      361 gtcgaggcgg cgctgaagaa cacgcacata ccggtgatgt tctggcgcag cgtcggttat
      421 tcgcagaaca cgttcgtgat ggagagcttc atcgacgaac tggcgcacgc agcgggcgag
      481 gatccgtacc agttccgccg caagctgctg gcgaacgagc ccgaatggct gcgcgtgctg
      541 gataccgtgg ccgagaaatc gggctggggc acgccgctgc cgaagggcag gggacgcggc
      601 atcgcgatac atatgtcgca tgatacgatc ctggccgaag tggccgaagt tgccgtcacc
      661 ccgggcggcg aggttcgtgt ggaacgggtt gtcgccgtcg ccgatcccgg ctgggtgatc
      721 aatccgcggc tgatcgagat gcaggtggaa agcggcatca tctacggcct gacgcggcgc
      781 tgtacggcgc cattaccatc aaggagggcg ccgtcgagca gagcaatttc gacaattacc
      841 gcatgatgct gatggcggac gcgccacgta tcgagacctg gctggtgcca agccatggca
      901 aacgatgggg cggcatcggc gaacccggca cgccgccggc ggccccggcg gtaacgaacg
      961 caatctttgc ggcgaccggc gtgcgcgtgc gcagtctgcc gatcgccacc gccgacctca
     1021 caagccgaag ctgaatcgca ggaggatcac catgccggcc gtatcgctca atcattatac
     1081 catcatggtg caagacctgg agcgcaccag ggaattctat gaagacgtcg tcggcctcac
     1141 caccggcgac cgcccgccgc tggcttttcc ggggtactgg ctttattgcg gtggggttcc
     1201 gacggttcat ttggtgggcc accgcccgga gaacccgcaa ttcgacggtg cgcccgaaac
     1261 cgggcggttg gatcacatcg ccttcgccgc ggaaaatctg aaactgatgc gcgaacgctt
     1321 gcagacgcgc ggactggaat tctcggaacg cgtgctgccg cgactggaca taacacagct
     1381 gttcttcaag gatccggacg ggatcaatat cgagttcaat ttccccgctg aggagacggt
     1441 gtgacacaag cggtccagcc cgcggtctga ttttggaccc tgagcggaca tctggcggcg
     1501 tcaattagat ggccgaagct gaccggaaaa ttggtcggag caaatcgccc ctgatgaggc
     1561 gcggtttctg tctcacgtgc ctgtcggtga ggcgctggac gcccgatatg cacccagcta
     1621 tccaattgcc agattcggcc tggcgcacgg atgcaaaagg gacggcactt tcacactgga
     1681 atcaagcaca gccctcgcga aaaggccgcg cgggttcacc ttggctcgct tgagttcaag
     1741 aagaaggggg tttctcgctt cgatttgcga gacccttacc atctcgccgt tgcactgacc
     1801 tggccacaat tcttcgcgac tttgctcgta acgtatttgt tggcgaacgt cttttttgca
     1861 agtttatttt ggttagtgcc cggttcggtg gccaatgctc ggccatatag ttttcccgac
     1921 gcgttcttct tcagcattga gacgctggcg acggtcggct atggcgagat gtatccggcc
     1981 acgctgtacg gccatataat cgcggcgacc gagattgtct gcgggctcgg cttcacagct
     2041 attctgacag gcctgatctt tgtccgattt tcgcggccac gggctaagtt ggtcttcgcc
     2101 acccatccgg tcgtggcgat gcacgagggc aagccgacgc tgatgttacg cgttgggaac
     2161 gggcgcgcag atgtgctttt ggatgcagtg acgaagctga acgtactgct aattgtaacg
     2221 acggcggaag gcaaactctt ccgccgcgcg caagaactgc ggcttgagcg tgcgcatctt
     2281 ccggttttcc cgctcacctg gacactcatg catgtgatgg acgagcgtag ccccttgtac
     2341 ggctacgacg cagcgcaagt ggttgcggcg ggcgcgcaag tgttcgtgac gctcgaagcg
     2401 cgcgatccga cgctcgcgac atcagtgcac gaaatccgca actatgcgcc ggaagatata
     2461 cgcttcggaa tgcgctatgt ggatgtgata acctcggaag aggacggcac gccggtggcg
     2521 gacctgacc
//
LOCUS       DATIMH010000535         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_708238, whole genome shotgun sequence.
ACCESSION   DATIMH010000535 DATIMH010000000
VERSION     DATIMH010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708238"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..801
                     /gene="infC"
                     /locus_tag="VLI90_09330"
     CDS             52..801
                     /gene="infC"
                     /locus_tag="VLI90_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSV14450.1"
                     /translation="MSTNFRHNEQIRISPVFLIDQDDNKLGSTSTADALRKAREAGLD
                     LVEVAPNARPPVCKIMDYGKWRYQQQKKADKSRASVRGGQLKEIKMKTVKIGDHDLQI
                     KINHAREFLKEGNKVQFTLQFRGREMAHLELGREIFKKIKDQLWQFSKIERDAKMEGR
                     RMTMVLQPDHKSPDAVKPVIGGTAPATARAGSGLNIPSRPTTGPVGPGVNRPAAAPAA
                     AGTSAPRPVAAPAAPAPARPAAAPQPAGAPR"
     gene            904..1185
                     /gene="rpmI"
                     /locus_tag="VLI90_09335"
     CDS             904..1185
                     /gene="rpmI"
                     /locus_tag="VLI90_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HSV14451.1"
                     /translation="MAYKYKPNKAVAKRFKVSKTGKLKRHHGFTSHLMSARPAGKRRH
                     LRKPEVLFEGHARNMRKLMGISKLKPAKVAHERALAAAEKAETSTAASA"
     gene            1329..1715
                     /gene="rplT"
                     /locus_tag="VLI90_09340"
     CDS             1329..1715
                     /gene="rplT"
                     /locus_tag="VLI90_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSV14452.1"
                     /translation="MPRTTGGPTHSRKRKRVLKRASGFVASPGTQYRQALEFTRRADR
                     YAYRDRRQKKRHFRQLWITRLGAACRARGINYNRFIPALTAAGIELNRKSLSDLAATD
                     PAAFDAIVELARPHLHVLNTAAPKAA"
     gene            complement(1793..2752)
                     /locus_tag="VLI90_09345"
     CDS             complement(1793..2752)
                     /locus_tag="VLI90_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HSV14453.1"
                     /translation="MKSIRVHAFGEPDVLKLEDAQDPKAAINQILIRVHAVGVNPVET
                     YIRSGKYGPKDFPFTPGNDAAGVVESVGPGVTKFKPGDRVYTDKTITGSYAELTLCET
                     NRVHALPANVSFPQGAAVGTPAGTAYRGLFQRGRGVAGETVLIHGATGGVGTSAVQLA
                     RAAGMTVVATASSDDGRKYARQHGAHHAVDHKITERADEVKSLTGGKGFDLILEMLAN
                     VNLASDLTAVATHGRVVVIGSRGKIEIDPRDTMRSEADILGLMLFGASEAEHRQMYAA
                     LTAALANGTLRPVIGMELPLAEAPRAHREVIEGDSHGKIVLIP"
     gene            complement(2832..3524)
                     /gene="lepB"
                     /locus_tag="VLI90_09350"
     CDS             complement(2832..3524)
                     /gene="lepB"
                     /locus_tag="VLI90_09350"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSV14454.1"
                     /translation="MPSRARRFWRQVCTFAFIFIVLAALRSAIADWNDVPTGSMIPTI
                     LEGDRIFVNKLAYDLKVPFTTMHLATWDNPRRGDVVVFFSPDEGTRLVKRVVAVPGDI
                     IELRDNVLYLNGVRSQYSAADAKAIDQLDEDTRLGKIFAAEREPNAAHAHPVMSIPAI
                     RDARRSFPPIVVPENQYFMMGDNRDNSRDSRYFGFVTRDRIVGRATAIVASLDLNHHW
                     VPRWGRFFKGLR"
     gene            complement(3938..>4678)
                     /locus_tag="VLI90_09355"
     CDS             complement(3938..>4678)
                     /locus_tag="VLI90_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV14455.1"
                     /translation="GRTMDGVVYKGSDVMWVYTGFPALSRVMLARFPQEEAEDRVAEI
                     LSYFKQWDAAVSWVVGPTSWPPKLGEYLHNQGFGSSELWTGMAVNLATLPEALDDRPA
                     GLRVQMVTDNAGLRSWATLSAEPGAKPEDEAAVGVFSADNAGSDARSRFYLAYLDGKP
                     VARCMSYNRGDTIGLYWLTTLPQHRGHGIAGAIGHRALADARDTGGRLAVMPAPLRSH
                     SLCERLGFRAYCQFSVYVWPPAPIPMPA"
BASE COUNT          908 a         1549 c         1431 g          790 t
ORIGIN      
        1 cgaggcgagt gcttcgcggc ggcaacggtt tttacacagg agtgaacggc catttccacc
       61 aacttccgcc acaacgaaca gatccggatt tcaccggttt ttctgatcga tcaggacgac
      121 aataagctcg ggtcgacatc gaccgctgat gcgctgcgca aggcgcgcga ggccggtctg
      181 gacctggtcg aagtcgcccc caatgcccgc ccgccggtct gtaagatcat ggactacggc
      241 aagtggcgct accagcagca aaagaaggcg gacaagtccc gcgccagcgt tcgcggcggt
      301 cagctcaaag aaatcaagat gaagaccgtg aagatcggcg accacgacct tcagatcaag
      361 atcaatcacg cccgcgagtt cctcaaggaa ggcaacaagg tgcagttcac gctgcagttc
      421 cgcgggcgcg aaatggccca cctggagctg gggcgcgaga tcttcaagaa gatcaaggac
      481 cagctgtggc agttcagcaa gatcgagcgc gacgcgaaga tggaaggccg gcggatgacg
      541 atggtcctcc agccggacca taagagtccc gacgcggtga aaccggtgat cggtgggacc
      601 gcccccgcga cggctcgcgc cggcagcggg ttgaacatcc cgtcccgacc cacgacaggg
      661 ccggtggggc ccggtgtgaa tcgtccagcg gcagcccccg cggcggccgg gacaagcgcg
      721 cctcgtccgg tggcggctcc agcagcgcca gcgccggccc gtccggctgc ggctccgcaa
      781 ccggccggag ccccacgcta gtcaccgcgt ttgccattcg ccccgcatac tgttactatc
      841 cccgccccct gaaaacgggg gcggttttat ttctgcgatc gatcacgaga ggactgactc
      901 ccgatggcgt acaagtataa gcccaacaag gccgtggcca agcgattcaa ggtcagcaag
      961 accggcaagc tcaagcgcca tcatgggttt acgtcgcacc tgatgagcgc ccgcccggcc
     1021 ggcaaacgcc ggcacctgcg caagcccgag gtgctgttcg aaggccacgc ccgcaacatg
     1081 cggaagctga tgggcatcag caagctgaag ccggcaaagg tcgcccacga gcgagcgctg
     1141 gcggcggcgg agaaggccga gacctcgacc gcggcgtcgg cataacacaa ttgaagattg
     1201 gcaatttttc aattgtcagt tgaagaacca agcgccggcg cgttgccggc gcagccaagc
     1261 ccccgcggga tccatcgtga tcgagcggcg agtgcctctg gctccgatga ctttgaaagg
     1321 acctgaccat gcctcgtaca acaggcggcc cgacgcacag ccgcaaacgt aaacgcgttc
     1381 tcaagcgagc cagcggcttc gtcgcctcgc cgggaacgca gtatcgtcaa gcgctcgaat
     1441 tcacccgccg cgcggatcgg tatgcatacc gcgatcgccg gcagaagaag cggcacttcc
     1501 gccagctgtg gatcacgcgc ctgggcgcgg cctgccgcgc tcgcgggatc aactacaacc
     1561 gcttcatccc cgcccttacc gccgccggga ttgaactgaa tcgcaagagc ctgagcgatc
     1621 tcgccgcaac cgaccccgcg gcgtttgatg cgatcgtcga gctggcgcgc ccgcatctgc
     1681 acgttctgaa caccgccgcg ccgaaagccg cgtgagcgct tcgagttctc gaaaaataaa
     1741 agcccacgca tagctatgcg tgggctttct ttttcttgcg cgcgcgatga tctcacggga
     1801 tcagcacgat cttcccgtgc gagtctcctt cgatgacttc ccgatgcgcc cgcggcgctt
     1861 ccgcgagcgg cagctccatg ccgatcaccg gacgcagcgt gccgttcgcc agcgccgccg
     1921 ttaacgccgc gtacatctgg cgatgctcgg cttcgctcgc gccgaacagc atcaagccga
     1981 ggatgtccgc ttcgctgcgc atcgtgtcgc gcggatcgat ctcgattttt ccgcggctgc
     2041 cgatgacgac gacccggccg tgcgttgcga cggccgtcag atcgctcgcc aggttcacgt
     2101 tcgccagcat ctccaaaatc agatcgaatc ccttgccgcc ggtgagcgac ttcacctcat
     2161 ccgcccgctc ggtgatcttg tgatcgaccg cgtgatgcgc gccgtgttgc cgcgcgtact
     2221 tccgcccgtc atccgacgac gcggtggcga cgacggtcat gcccgccgcc cgcgccagct
     2281 ggacggcgct ggtgcccacg ccgccggtgg cgccgtggat cagcactgtt tcaccggcga
     2341 cgccgcgacc gcgctggaag agtccacgat acgccgtccc ggccggtgtg ccgacggcgg
     2401 cgccttgcgg gaacgacacg ttcgcgggca gcgcgtgaac tcgatttgtc tcgcaaagag
     2461 tcaactcggc gtacgagccg gtgattgtct tgtcggtgta aacgcgatcg ccgggtttaa
     2521 acttggtgac gccagggccg acggattcca caacgccggc ggcgtcgtta ccgggagtga
     2581 aggggaagtc tttcgggccg tacttgccgg agcggatgta cgtctcgacg ggattgacgc
     2641 cgacggcgtg aacgcgaatc agtatttggt ttatcgcggc cttggggtcc tgcgcgtctt
     2701 cgagcttgag aacgtctggc tcgccgaagg cgtgaacgcg gatggatttc atgccgggat
     2761 tgtagccgct tgcggctttg catctgttcc ctgccggatc aggaaaagat gctaagccgc
     2821 aagcggccag atcaccgcag ccctttgaag aatcgtcccc agcggggcac ccaatgatga
     2881 ttcagatcca gcgatgccac aatcgccgtc gcgcggccga cgatgcggtc gcgggtcacg
     2941 aagccgaaat agcgcgaatc ccggctgttg tcgcggttgt cgcccatcat gaaatactga
     3001 ttctccggaa cgacgatcgg cgggaacgac cgccgcgcgt cgcggatcgc gggaatcgac
     3061 atcaccggat gcgcgtgagc cgcgttgggc tcgcgttcgg cggcgaagat ttttccgagc
     3121 cgcgtgtcct cgtccagctg gtcgatcgcc ttcgcgtcag ccgcgctgta ttgggatcga
     3181 acgccgttca gatacagcac gttgtcgcgc agctcgatga tgtcgcctgg cacggccacg
     3241 acgcgtttca ccagccgcgt gccttcatcg ggcgagaaga agacaacgac atcgccacgc
     3301 cgggggttgt cccacgtcgc caggtgcatc gtcgtgaacg gcaccttcag gtcgtacgcc
     3361 agcttgttca cgaaaatgcg atcgccttcg aggatggtgg ggatcatcga tcccgtcggc
     3421 acgtcgttcc aatcggcgat ggccgaccga agggcggcca ggacgatgaa gatgaacgcg
     3481 aaggtgcaga cctgccgcca gaaccgccga gcgcgcgagg gcatgggacc ggtcgcgaca
     3541 gaagcaggcg aagccggcgg cggcgggagt gggttcgaca tctcgctcat gagtcgtaca
     3601 gataaccgct ggatcgtagg catcaagcaa agagacgccg acgaaccgtc gggcgcttct
     3661 gtaagtgtga atccatcccc acgccgggtt cgcagcgcct gccctgagct cgtcaaaagg
     3721 tacccgcgac gaagcgagat tccgaggtcg atgatcgacg ctcaaagcga agcgcaacaa
     3781 aaaaatcttc gcaagttgcg cggactaaaa cacctgcaag ccgctgctat ttaaagagat
     3841 atgctggcgt ccctgcctca gaatcgccaa accaggttgc gcttcatcgc attttgtccc
     3901 catcttgccc agcctatcca aattccggcc cttctgctca cgccggcatc ggaatcggcg
     3961 cgggcggcca cacataaacg ctgaactggc aatacgctcg gaacccaagc cgctcgcaaa
     4021 gcgaatggct ccgcagcggc gccggcatca cagccagtcg cccgccggtg tcgcgcgcat
     4081 ctgccaaagc tcgatgcccg atggcgcctg cgatgccgtg cccgcgatgc tggggcagcg
     4141 ttgtcaacca atacagcccg atcgtatcgc cgcggttgta cgacatgcag cgggcgacgg
     4201 gtttgccgtc gagatatgcc aggtaaaacc gcgatcgcgc atcgctcccc gcgttgtccg
     4261 cggagaagac gcccacagcc gcttcgtctt ccggcttggc accaggttca gcagacaacg
     4321 tcgcccacga ccgcagcccc gcattatccg tcaccatctg cacccgcagc cccgccggtc
     4381 gatcgtccag cgcctccggc agcgttgcca ggttcaccgc catgccggtc cacagctcgc
     4441 tcgaaccaaa cccctggttg tgcagatatt cccccagctt ggggggccag ctcgtcgggc
     4501 cgaccaccca cgacacggcc gcgtcccatt gcttgaaata gctcaggatc tccgccaccc
     4561 ggtcttccgc ctcttcctgc ggaaaccggg ccagcatcac ccggctcagg gccggaaagc
     4621 cggtgtaaac ccacatgacg tccgagccct tgtagacgac gccgtccatc gtccggcc
//
LOCUS       DATIMH010000536         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3594070, whole genome shotgun sequence.
ACCESSION   DATIMH010000536 DATIMH010000000
VERSION     DATIMH010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3594070"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VLI90_09360"
     CDS             <1..1386
                     /locus_tag="VLI90_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14456.1"
                     /translation="ARSFDSGGVPPALEDDGFSLRGRIISESREFTMTNVYTLNLNSS
                     DLTGFAAAPLSFAPTTRNAANIAGFATTGVPGFAAPNPGTFEVYRRISAHPTVALAMR
                     IVTGPIVANGWGWAKRRDVPEQWLAFVRDMLQPMRTRVLEHALRALVFGFAGFEKVWE
                     VDGGRLRIAKLKPLYPDITQVLVDDGGNFRGFRQQGPGDATPRDVLGSKAFCFTYDGE
                     CGDPYGRSRHENVRVVWSQAMQTAERLAQYHKKIAGVIAQLHYPEGTSRDAAGVERSN
                     DWIAQQILEAVSMGRSVRFPNLFASIDDPKSAAELAGKSQWVLSQFDPGGTDHSAGLL
                     AALAYYDKQIFRGWLRPERVALEAQHGSRADAMEHTETGIVDSELVDRAFASAFNAGV
                     VDDVLTLNFGPAARGAVWVEPSPISDSRTSTLRSLLAALINHAQFATDLAQRVDVEAM
                     LADLDVPTKSA"
     gene            1553..2035
                     /locus_tag="VLI90_09365"
     CDS             1553..2035
                     /locus_tag="VLI90_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14457.1"
                     /translation="MEPIMPPSEIKNRQVKVTEDDRQVVLHPENDDLFVQTGKQVIDA
                     CRLNISIELWIHELTALSAHVQEWARARSSRIASIYLAPRPNKLVLFVCPASKEFDFD
                     LADEMAQLNTALTKNFNVGTVEILQVPAEELDRFVDGGGGKAKLLYGRQAESHQPVEA
                     "
     gene            1995..2435
                     /locus_tag="VLI90_09370"
     CDS             1995..2435
                     /locus_tag="VLI90_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14458.1"
                     /translation="MADKQSHINQWKHNRKFISSIDPEFHDWIITAVFYTAVHAVDTL
                     LAHDKVTVTDHEARNRALKLTNRYEQINLKYEALYGLARTIRYFANPTKWVAPERIYK
                     DVVVRYLYPIEASVQKHMGQDLGLGDVKLKVGTKSGAGSSSTPT"
     gene            complement(2484..>2702)
                     /locus_tag="VLI90_09375"
     CDS             complement(2484..>2702)
                     /locus_tag="VLI90_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14459.1"
                     /translation="DGSFVAFFPTFYGKGVTIGGLVYTSRPLRLEDTYVRALGTTLDR
                     RFIDVGPWNKLSVDSRVNEHWYRVSRGI"
BASE COUNT          550 a          804 c          861 g          487 t
ORIGIN      
        1 gcaagatcct tcgactccgg cggagtaccg cctgcgctgg aggatgacgg tttcagcctt
       61 cgcggccgca tcatttcaga aagccgggag ttcaccatga ccaatgtcta cacgctcaac
      121 ctcaacagca gcgacctgac cggcttcgcc gcggcgccgc ttagcttcgc gccgacgacc
      181 cggaacgccg cgaatatcgc ggggtttgcc acgacgggtg tgccgggttt cgcggcgccg
      241 aatccgggga cgttcgaggt ctaccgccgc atttcggcgc atccgacggt ggcgctggcg
      301 atgcggatcg tcaccggccc gatcgtcgcc aacgggtggg gctgggccaa acgccgcgac
      361 gtgccggagc agtggctggc cttcgtgcgc gacatgctgc agccgatgcg cactcgcgtg
      421 ctcgagcacg cgctgcgggc gctggtcttc ggcttcgcgg gatttgaaaa agtgtgggaa
      481 gtcgatggcg ggcgattgcg gatcgccaag ctcaagccgt tgtatcccga catcacgcag
      541 gtcctggtgg acgacggcgg gaacttccgg ggttttcgcc agcaggggcc gggcgacgcg
      601 acgccgcgcg acgtgctggg gtcgaaggcg ttttgcttca cgtacgacgg cgagtgcggt
      661 gacccgtacg gccgcagccg gcacgaaaac gtccgcgtcg tctggtcgca ggcgatgcag
      721 acggccgagc ggctggcgca gtatcacaag aagatcgcgg gcgtgatcgc gcagctgcat
      781 taccccgagg gaacgagccg agacgccgcg ggcgttgagc gaagcaacga ctggatcgcg
      841 cagcagatcc tggaagcggt cagcatgggc cgcagcgtgc ggtttccgaa tctgttcgcg
      901 tcgatcgacg atccgaagtc agccgcggag ttggccggca aaagccaatg ggtgctgagc
      961 cagttcgacc ccggcgggac cgatcattcc gcgggtttac tggcggcgct cgcatactac
     1021 gacaagcaaa tcttccgcgg ctggctgcgg cccgaacgcg tggcgctgga agcgcagcac
     1081 ggttcgcgcg ccgacgcgat ggaacatacc gagaccggca tcgtcgacag cgagctggtc
     1141 gatcgcgcgt tcgcgtcggc gttcaacgcc ggcgtggtgg atgacgtgct gacactgaac
     1201 ttcggccccg ccgcgcgcgg ggcggtgtgg gtggaaccgt cgccgatctc ggatagccga
     1261 acgtctaccc tcagatcgct actggcggca ttgatcaacc acgcgcagtt cgcgacggac
     1321 ctggcgcagc gcgtggacgt ggaggcaatg ctggcggatc tggatgtgcc gaccaaatca
     1381 gcttgagcgt tggttccttg acagcaatcg cgcgccacga cgatacttca gtggagaaac
     1441 ctgcgattat gccgtacgat tgtgtgatct aacgacgata agtgttcctt tcgcccatcc
     1501 caggagccgt ggtcaacgag gccgctgctc aatagtcgat gaggatcaga cgatggaacc
     1561 catcatgccg ccatcagaaa ttaaaaaccg acaagtaaaa gtgacggagg acgatcgcca
     1621 ggtcgtgctc catccggaga acgacgatct gttcgtgcag actggcaagc aggtcatcga
     1681 tgcgtgccgg ctaaacatca gcatcgaact gtggattcat gagctcaccg cgttaagcgc
     1741 gcacgtgcag gaatgggcgc gggcgcgctc cagcagaatc gcatcaattt accttgcgcc
     1801 acggccgaac aagctggtgc tttttgtctg cccggcgtcg aaggaatttg atttcgattt
     1861 ggcggatgag atggcgcagc tgaatacggc attgacgaag aacttcaacg tcgggacggt
     1921 cgaaatcctt caagttccgg cggaggaact tgaccgattt gttgacgggg gcgggggcaa
     1981 ggcgaagctt ctctatggca gacaagcaga gtcacatcaa ccagtggaag cataaccgaa
     2041 agttcatttc ctcgatcgat ccggagtttc atgactggat catcaccgcc gtcttctaca
     2101 ccgccgtgca cgccgtcgat acgctgctag cgcacgataa ggtgacggtc accgatcacg
     2161 aagcgcgaaa ccgggcactg aagctcacga accgctacga gcaaatcaat ctcaagtacg
     2221 aagcgcttta cggccttgcc aggacgatcc ggtactttgc caatcccacg aaatgggtgg
     2281 cgcccgagag gatctacaag gacgtcgtgg tgaggtacct ctatccgatt gaagcttcgg
     2341 tccaaaagca catgggtcag gatcttgggc tcggagatgt gaagttgaaa gttgggacca
     2401 agtccggcgc tggctcgtca tcgacgccga cttaaccaag tgccgcgact ccgcaccggt
     2461 acatgctgcg cgcacgatgg attctatatc ccccgcgaca ctcgatacca gtgttcgttc
     2521 acccgcgaat ccacgcttag cttattccac ggccccacgt cgatgaaccg ccggtccagc
     2581 gtggtgccca gcgcgcggac gtacgtgtcc tccaaccgca gcgggcggct ggtgtacacc
     2641 aggccgccga tcgtcacccc tttaccatag aacgtcggga agaacgcgac gaagctgccg
     2701 tc
//
LOCUS       DATIMH010000537         1703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4169506, whole genome shotgun sequence.
ACCESSION   DATIMH010000537 DATIMH010000000
VERSION     DATIMH010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4169506"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VLI90_09380"
     CDS             complement(<1..1317)
                     /locus_tag="VLI90_09380"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HSV14460.1"
                     /translation="MSAFVRRRHRRWDRIGSFGVDVLEGRVLLAATPLATQSSFFSVN
                     TGSSAGPATFAGATVLPSGAKHLLSFDNSATFLPLQTYSFAFSKTITIAGGAGGITES
                     KINVTPLSITLSERLGTAGAFVSMLSGSVANQVSLLDFDKQGKQYTKWDFSDVTVSSY
                     SVSAGGASDTGPIPLPTEAIQLSFDKVQLSNIDPNTGVVVGTPITWKLDTATPGAALA
                     ASVNSPVATAAAVPAPKPNSTQMIVDPNGNATAFDLDTASLGTARNTVTEFSATLTNP
                     LQMVPMLANVLNGIFQTVTTTAYDSATKPLVTWEMNDADFTSVSVSASAGQNPLTSLT
                     TAAVKVTQTTPDNSQSPAPTPDVWSFDQAARTVTGTASFAGPATIPVTQAEHTLADPG
                     VGSGLLPVYSTQLGSQNNAGTKVLSGFSLQTDLTIGSAGLMADAVRG"
BASE COUNT          310 a          557 c          540 g          296 t
ORIGIN      
        1 cccgcgcacc gcgtcggcca tcaggccggc gctgccgatc gtcaggtcgg tctgcaaact
       61 gaaaccgctc agcaccttcg tgccggcgtt gttctgcgaa ccgagctgcg tgctgtagac
      121 cggcaacagc ccgctgccca cacccgggtc ggcaagcgtg tgctcggcct gcgtcacggg
      181 aatcgtcgcc ggccccgcga agctggcggt gccggtgacc gtccgcgcgg cttggtcgaa
      241 cgaccacacg tccggcgtcg gcgcgggcga ttgcgaattg tccggcgtcg tctgcgtgac
      301 cttcacggcg gccgtcgtga gcgacgtcag cggattctgc ccggcgctgg cgctgaccga
      361 caccgatgtg aagtcggcat cgttcatttc ccacgtcacc agcggcttgg tcgcgctgtc
      421 gtacgcggtg gtggtcaccg tttgaaatat gccgttcaac acattggcca gcatcggcac
      481 catctgcagc gggttggtca acgtggccga gaattcggtg acggtgttcc tggcggtgcc
      541 gaggctggcc gtgtcgaggt cgaaggcggt ggcgttgccg ttgggatcga cgatcatctg
      601 cgtgctgttg ggctttggcg cgggcacggc cgcggcggtg gcgacgggtg agttcaccga
      661 tgccgccaac gccgcgccgg gcgttgcggt atcgagtttc caagtgatcg gtgttcccac
      721 gacgacgccg gtgttcgggt caatgttcga tagctgcact ttgtcgaatg acaattggat
      781 cgcttcggtc ggcagcggga tcggcccagt gtcactggcg ccgcctgcgc tcacgctgta
      841 gctgctcacc gtcacgtcgc tgaagtccca cttggtgtat tgcttgccct gcttgtcgaa
      901 atccagcagc gagacctggt tcgcgacgct gccgctcagc atcgacacga atgccccggc
      961 cgtgccgagc cgctcgctca gcgtaatgct caacggcgtg acgttgattt tcgattcggt
     1021 aatcccccca gcgccaccgg caatcgtgat cgtcttcgag aatgcgaacg aataagtctg
     1081 cagcggcaaa aacgtggccg agttgtcgaa gctcagcaaa tgcttcgccc ccgacggcag
     1141 caccgtcgcc cccgcaaacg tcgccggccc cgcgctcgag ccagtgttca cgctaaagaa
     1201 cgaagactgc gtcgccagcg gcgtcgccgc caacaacacc cgcccttcca agacatccac
     1261 gccaaacgaa ccgatgcgat cccaacgacg atgcctgcgg cgaacgaacg cggacatgct
     1321 cggcctccca aacaaaatgg cagaagtgaa gatcgcgcgc acggtcctat gaaaccccgc
     1381 gcacgccacc acgcgcgcga acgccaataa agcccgcgcg gcggaggggg ggattataag
     1441 aagttgatga gagggcgaaa agcgaaaagc gggggcggac gaagagaaaa gcgggacgcg
     1501 ctgagcaggg ccgccgcagc cggccgcgtg tctaatgtcg agttgctgct cgaattaccg
     1561 cactccgctg ccccgcggtc agcggatcca cattcctcag ccaggccgcc ccgtaggttg
     1621 atccaaggcg atcgcgtatt ccagcgcatg attgaaccgc gtctgctccg acgcccgcat
     1681 ccagcaacgg acctacacgc cgc
//
LOCUS       DATIMH010000538         3844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3880293, whole genome shotgun sequence.
ACCESSION   DATIMH010000538 DATIMH010000000
VERSION     DATIMH010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3880293"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2510
                     /locus_tag="VLI90_09385"
     CDS             <1..2510
                     /locus_tag="VLI90_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV14461.1"
                     /translation="DFARDAAPRQWYEPLVPEDLPALSHPKYFNDLDKAKADAFAGRY
                     RLALQTLRSIKDGDATTIALIKGESLAATGHLDEALAALAALASDDPRVQLARVRVLE
                     QLGRTADAAALLHQHLKDHPNSLAGHFELGRLCESIGDFGTAKTAYEWFVAPGQDYLE
                     KWRGQHEKLFEKAEDATTLGRALDRWATLTGAYQHDSALHNTILDLFVKSYDVIDRGY
                     WPAHLAAAEYFLAHDDEDNAKKELRVALRANPYSADALRLAGRIAIENYDFDNADHVV
                     DALRRENPNSIQADLLQARNLLRQRLPKTAGEIARKVLQRQPKQLEAMGLVAACEALQ
                     LHDDATKQLLADVDAVAPHLPSAYFDVAEQLAAMRQYPRSAAMYQVALDRAPWWTAAR
                     NGLGLLYTQSGDEDLARSTLDAAHQLDPYNLSTTNYLRLLDELAAFDKKESAHFIVMY
                     DGKLDPMIPEYFTDYLESIYPLVTREYHTEPPVKTYIEVFPTHDAFSVRTTGSPWIGT
                     VGASTGRVIAMVSPRKGENTQGAFNWSQVLRHEFTHTVTLAATDNRISHWMTEGLAVM
                     EEHSPLQWAWVPMLYHAVSHHELFDMDALTWAFIRPRKPSDRQMAYAESFWVCTYIEQ
                     TYGHDAILKMLAEFKAGGSQEDVFPKILGRSQTEFFSEFSQWTQRQIAGWGYDEQSQK
                     KYDELRDQAEELIKTREYPEALKLWEQIAKLRPVDQLPHQRLAGLYLSKAVNEPQKAV
                     DHLKILHQVDLKDDRYAKRIARLYRDMNKLDDARHWAMQAIYIDPYDMDAHDLMAELC
                     EKADDAAGLAREQRVIPLLKQWMQNAKKTSAVPNEAQ"
     gene            2510..2797
                     /locus_tag="VLI90_09390"
     CDS             2510..2797
                     /locus_tag="VLI90_09390"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HSV14462.1"
                     /translation="MTNANDLTIANFQRHIFDKYEKVDRARGTPKTFLWFIEEVGELA
                     TALNGDDRANLEEEFADVIAWLCTLANINDVDLASAVAKKYLSEKPPQGHK"
     gene            2809..3426
                     /locus_tag="VLI90_09395"
     CDS             2809..3426
                     /locus_tag="VLI90_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14463.1"
                     /translation="MSEAETNIVEDGEAPLPVLKVDEDPPAVLAYANLRGAQPAHSRF
                     VCQRHTDGSVTLMEPPDLSQSLGTRVAGVLAVLLAIAWVIWLLYDLLRTRWQLIWPAV
                     IIAPLVLAALGMLLWLEGRYAARQPLVIKARPGEIIVHHRTMLRFGRRSVLRGRRLGR
                     IVVPLGSPSLKSMRMHSSVYVSPVLGLATPLLANRRMSACGSRRF"
     gene            complement(3556..>3844)
                     /locus_tag="VLI90_09400"
     CDS             complement(3556..>3844)
                     /locus_tag="VLI90_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HSV14464.1"
                     /translation="LPPQAFWPAPKIESALVRMTREDRLGNRAAPFGNFIRKVFSARR
                     KTMRKAMTLAGLPAEQLLERAGLDGQRRPEEFSPEQLWQLFELGYAASGSE"
BASE COUNT          787 a         1233 c         1188 g          636 t
ORIGIN      
        1 cggatttcgc gcgcgatgcg gcgccgcggc aatggtacga accgcttgtg ccggaagacc
       61 tgccggcgct cagccatccc aaatatttca acgacctgga caaagccaaa gccgacgcgt
      121 tcgccgggcg atatcggctg gcgctgcaga cgctgcggtc gatcaaggac ggcgacgcga
      181 cgacgatcgc gctgatcaaa ggcgaatccc tcgccgccac cgggcatctc gatgaagcgc
      241 tggcggcgct ggcggcgctg gcgtcggatg acccgcgggt ccagctggcg cgcgttcgcg
      301 tgctggagca gctcggccgc accgccgacg cggcggcgct gcttcatcag cacttaaaag
      361 atcatccgaa ttccctcgcc ggccacttcg agctcggccg gctgtgcgaa tccatcggcg
      421 atttcggcac cgccaaaacc gcctacgaat ggttcgtcgc gccgggccag gactacctgg
      481 aaaaatggcg cggccagcac gagaagctgt tcgaaaaagc ggaagatgcg accacgctcg
      541 gccgcgcgct cgaccgctgg gccacgctca ccggcgcgta tcagcacgac agcgcgctgc
      601 acaacaccat cctcgacctg ttcgtcaaat cgtacgatgt catcgatcgc ggctattggc
      661 ccgcgcacct ggcggccgcc gaatatttcc tggcgcacga tgacgaggac aacgccaaga
      721 aagaactgcg tgtcgcgctg cgggcgaacc cgtactcggc cgatgcgctg cgattggccg
      781 gtcgcatcgc gatcgagaat tacgattttg acaacgccga tcacgttgtt gatgcgttgc
      841 gacgtgagaa tcccaattcg attcaggcgg atctgctgca ggcgcgaaac ctgcttcgcc
      901 agcggctgcc caagacggcg ggggagatcg cgcgaaaggt gctgcagcgc cagccgaagc
      961 agctggaagc gatgggcctc gtcgcggcgt gcgaagcgct gcagctgcac gatgacgcga
     1021 cgaaacagtt gctcgcggat gtggacgcgg tcgccccgca tctgccgagc gcctacttcg
     1081 acgtcgccga gcagttggcc gcgatgcggc aatacccgcg atcggcggcg atgtatcagg
     1141 tcgcgctgga tcgcgccccg tggtggaccg ccgcccgcaa cgggctcggt ttgttgtata
     1201 cgcagagtgg cgatgaagac ttggcgcgtt ccacgctcga tgccgctcat caactcgatc
     1261 cgtacaacct ctcgacgacg aactaccttc gcctgctcga tgaactggcg gcgttcgaca
     1321 agaaggaatc ggctcacttc atcgtgatgt atgacggcaa gctcgacccg atgattcccg
     1381 aatatttcac ggattatctc gagagcatct acccccttgt cacgcgggaa tatcacaccg
     1441 agccgccggt gaaaacgtac atcgaagtct ttcccacgca cgatgcgttc agcgtgcgca
     1501 cgacgggttc gccgtggatc ggcaccgtgg gcgcgagcac cgggcgggtg atcgcgatgg
     1561 tgtcgccgcg caaaggcgag aacacgcagg gcgcgttcaa ctggagccag gtgctgcggc
     1621 acgaattcac gcacaccgtc acgctggccg ccaccgacaa tcgcatttcg cactggatga
     1681 ccgaaggttt agcggtgatg gaagagcact cgccgctgca gtgggcatgg gtgccgatgc
     1741 tgtatcacgc ggtcagccat catgaattgt tcgacatgga cgcgctcacg tgggcgttca
     1801 tccgcccgcg aaaaccgagc gatcgccaga tggcgtacgc cgaatcgttc tgggtctgca
     1861 cctacatcga gcagacctac ggccacgacg ccatcctgaa aatgctggct gagttcaaag
     1921 ccggcggatc gcaggaagac gtcttcccca aaatccttgg ccgcagccag acggaattct
     1981 tcagcgaatt ttcgcagtgg acgcagcggc aaatcgccgg ctggggatac gacgagcaat
     2041 cacagaagaa gtacgacgag ctgcgcgacc aggcggaaga gctgatcaaa acgcgcgagt
     2101 atcctgaggc gctgaagctg tgggagcaga ttgccaagct gcgcccggtc gaccagctgc
     2161 cgcaccagcg gctggccggg ctgtacctga gcaaggcggt gaatgagccg caaaaggcgg
     2221 tcgatcacct gaagatcctg caccaggtcg acctgaagga cgaccgctac gccaagcgaa
     2281 tcgcccggct gtatcgcgac atgaacaagc tggacgacgc ccggcactgg gcgatgcagg
     2341 cgatctacat cgatccgtac gatatggacg cccacgactt gatggccgag ctgtgcgaaa
     2401 aggccgacga cgccgcgggg ctcgcgcgcg agcagcgcgt gatcccgctg ctgaagcagt
     2461 ggatgcaaaa cgcgaagaag acctcggcag tccccaacga agcgcaatga tgaccaacgc
     2521 caacgacctg acgatcgcca actttcagcg gcacatcttc gacaagtacg aaaaggtaga
     2581 ccgcgcccgc ggcacgccga aaactttttt atggttcatc gaagaagtgg gcgaattggc
     2641 gacagccctg aatggcgacg atcgcgcgaa cctggaagaa gaattcgccg acgtcatcgc
     2701 ctggctctgc acgctggcga acatcaatga cgtggatcta gcatcggcgg tggcgaagaa
     2761 atatctgagc gaaaaaccgc ctcaagggca taaatagcgg cgcgtcgcat gtccgaagcc
     2821 gagaccaaca tcgttgaaga cggcgaagcg ccgctgccgg tgttgaaggt ggacgaagat
     2881 cccccggcgg tgctggcgta tgcaaacctc cgcggcgcgc agccggccca cagccggttc
     2941 gtctgccaac gccacaccga cggcagcgtg acgctgatgg aaccaccgga tctatcgcaa
     3001 tccctcggca cgcgcgtcgc cggtgtgctt gcggtactgc tggccatcgc gtgggtgatc
     3061 tggctgttgt acgacttgct ccgcacccgt tggcaactca tctggcccgc cgtcatcatc
     3121 gcgccgctcg tactggctgc gctgggcatg ctgttatggc tcgaagggcg atatgccgcc
     3181 cgacagccgc tggtcatcaa agcgcgcccc ggcgagatca tcgtgcacca tcgcaccatg
     3241 ctccgcttcg gccgccgcag cgttctgcgc ggccgccggc tgggacggat cgtcgtcccc
     3301 cttggcagcc ccagcctgaa gtcgatgcga atgcacagca gcgtgtacgt ctcacccgtg
     3361 ctcggcctgg cgacgccgct gctggcgaac cgtaggatga gtgcgtgtgg atcgcgaaga
     3421 ttctgagtga agcaactcag ccgtcctcca gcgcaggcta ctcgccggag tcgaaggatc
     3481 tggctcaaag tgattaaact gttatctgcg caattggtgc gagcgacgcg tcgcgccgtt
     3541 cgcgcaaccg ccggttcact cacttccaga cgcggcgtaa cccagctcga acaactgcca
     3601 caactgctcc ggcgaaaatt cctccggccg gcgctggccg tccaatcccg cgcgctcaag
     3661 caactgctcc gccggcaggc ccgccagcgt catcgccttt cgcatcgtct tgcgccgcgc
     3721 cgaaaaaacc ttgcgaatga aattgccaaa cggtgccgcg cgattgccca gccggtcctc
     3781 acgcgtcatc cgcaccagcg ccgattcgat cttcggcgcc ggccaaaacg cctgcggcgg
     3841 cagc
//
LOCUS       DATIMH010000539         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4170808, whole genome shotgun sequence.
ACCESSION   DATIMH010000539 DATIMH010000000
VERSION     DATIMH010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4170808"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..2579
                     /gene="rnr"
                     /locus_tag="VLI90_09405"
     CDS             228..2579
                     /gene="rnr"
                     /locus_tag="VLI90_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HSV14465.1"
                     /translation="MSEQLKNEIINHLKSSAYRPQRPRGLAQELDVADEAQYHAFRDA
                     LRELMHAGRVVLGARGAIMLPGEVTRDGLVTGTYRHNKRGFGFVVPTDPRGHEDLFIP
                     EGQNYGAITGDIVRAKITDRGQRDGKALFGGRITEIVKRTQKRFVGSLAKVHGEWLVY
                     PDGNALTEPIQTPDAASRHIKPGTKVVVELTTYPGEADGRPAGVITEVLGPAGQKDVD
                     LKSVIVQFNLPGPFEEPVKEQARVAIDDFDPESERRERLDLTDQIICTIDPDDAKDYD
                     DAISLRRLEDGNWELGVHIADVSYFVPAETPLDLEAKERGNSCYFPGFVIPMLPEVLS
                     NGVCSLQEAVPRLCKSAFITYDETAKPIRTKFANTIIKSRKRLRYREAQAILDGAEAI
                     PHPQGNRSINDYEPDVIELLHQMNKLAKRLQKRRQSEGQIVLELPETELVLDEEGKVI
                     DAVPEDTSFTHTLIEMFMVEANEAVARLLDSLDVPFLRRAHPEPKPESSERLRTFVQL
                     AGFRMGKEIDRKAIQSLLASVKGNPESFTINLAVLKSLSRAEYSPQPIGHYALASEYY
                     CHFTSPIRRYADLTIHRLLDEYFAARERPEHAGGRNRKKKIVLEDAPSYDELVELGRH
                     LSFTERRGDDAERELRQVKLLELLKNHIGDEFLGVVTGITNFGVFIQLQTYLIDGLIR
                     YENLMDDWWDVDERAGVVRGQRTGKKIGIGDQCKVIVARVDPARRELDLAISQLIGKA
                     GHPSPSVKPTKKITKKGKSEKHAPRGGATKRNQRSKQRGRRGR"
     gene            2639..2839
                     /locus_tag="VLI90_09410"
     CDS             2639..2839
                     /locus_tag="VLI90_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14466.1"
                     /translation="MSGITEVERAVRELPPAELAAFREWFAQFDAAMWDRQIEADVSA
                     GRLDAFADEMLHDLRDGRCKEL"
BASE COUNT          613 a          859 c          893 g          479 t
ORIGIN      
        1 ctgccagacc ctttttgccg atgctgtaca tcagaccatt gccggtatat gctttggtca
       61 gttagactga acgcgttccg cttccgcagg tgaacttccc cgccggtttc cccgcctgag
      121 attgttgatc cgttccgcac aatacaaatt gttgattccg cccggtcgcg attagacttt
      181 tgcaccggtg gccggcgatc atttcatcga ccttttagaa agaacgcatg tccgaacaac
      241 tgaagaacga aatcattaat catctcaaat ccagcgcgta ccgcccgcag cggccgcggg
      301 ggttggcgca ggaactggat gtcgccgacg aggcgcaata ccacgcgttt cgcgatgcgc
      361 tgcgcgagct gatgcacgcc ggccgggtgg tgctcggggc gcgcggcgcc atcatgttgc
      421 cgggcgaggt gacgcgcgac ggcctcgtca ccggcaccta tcgccacaat aagcgaggct
      481 tcggcttcgt ggtgccaacc gacccgaggg gtcacgaaga cctgttcatc cccgaaggcc
      541 agaactatgg cgcgatcacc ggcgacatcg tccgcgcgaa gatcaccgac cgcgggcagc
      601 gcgacggcaa agcgctgttt ggcgggcgca ttaccgaaat cgtcaagcga acccaaaagc
      661 gcttcgtcgg ttcactggcg aaggtgcacg gtgaatggct ggtttatccc gatggcaacg
      721 cgctgacgga accgatccaa acgccggatg ccgcgtcgcg gcacatcaag ccggggacga
      781 aagtcgtcgt cgaactgacg acatatcccg gcgaagccga cggccggccc gcgggcgtga
      841 tcaccgaggt gctcggcccc gcagggcaga aagatgtcga tctgaagtcg gtcatcgtgc
      901 agttcaatct gccggggcca ttcgaagagc ccgtgaagga gcaggcccgg gtggcaatcg
      961 atgacttcga tccggaaagc gaacggcgcg agcggctcga tctgaccgac cagatcatct
     1021 gcacgatcga ccccgatgac gcgaaggact acgacgacgc gatcagcctt cggcggctgg
     1081 aagacggcaa ctgggaactg ggcgtgcaca tcgccgatgt ctcgtacttc gtgccggcgg
     1141 aaacgccgct ggatctcgaa gcgaaagagc gcggcaacag ctgttatttc cccggcttcg
     1201 tcatcccgat gctgccggaa gtattgagca acggcgtctg ttcgctgcag gaagccgtgc
     1261 cccggctgtg caagagcgcg tttatcacgt acgacgaaac cgccaagccg attcgaacga
     1321 agttcgccaa cacgatcatc aagagccgca agcggctgcg ctatcgcgaa gcgcaggcga
     1381 tcctcgacgg cgctgaagca atcccgcacc cgcagggtaa tcgtagcatt aacgactacg
     1441 agccggacgt catcgagctg ctgcaccaga tgaacaagct ggccaagcgg ctgcagaagc
     1501 gccgccagtc ggaaggccag atcgtgctcg aactgccgga gaccgagctg gtgctggatg
     1561 aagaaggcaa ggtcatcgac gcggtgccgg aagacacgag cttcacgcat acgctgatcg
     1621 agatgttcat ggtcgaggcg aacgaagcgg tggcgcgatt actcgacagt ttggacgtgc
     1681 cgttcctccg ccgggcgcat cccgagccca agcccgagtc atccgagcgg ctgcgcacgt
     1741 tcgtgcagct ggccggcttc cgcatgggca aggagatcga tcgcaaggcg atccagtcgc
     1801 tgctggcatc cgtgaaggga aatcccgaat cgttcaccat caacctggcg gtgctcaaga
     1861 gcctgtcgcg cgcggaatat tcgccgcagc cgatcgggca ttacgcgctg gccagcgaat
     1921 attattgcca cttcacctcg cccattcggc gttacgccga cctgacgatc catcgcctgc
     1981 tcgacgaata tttcgccgcc cgcgaacgtc ccgagcacgc gggtgggcgc aatcgcaaga
     2041 agaagatcgt gctggaagac gcgccaagtt atgacgagct ggtcgagttg ggccggcact
     2101 tgagcttcac cgaacgccgc ggcgatgacg ccgagcgcga gctgcggcag gtcaagctgc
     2161 tcgaactttt gaagaaccac atcggcgacg aatttctcgg cgtcgtcacc ggcatcacca
     2221 acttcggcgt cttcatccag ctgcagacct atctgatcga tggcctgatc cgctacgaaa
     2281 acctgatgga cgattggtgg gacgtcgatg aacgcgccgg tgtcgtccgc gggcaacgca
     2341 cgggcaaaaa gatcggcatc ggcgatcagt gcaaggtgat cgtcgcccgc gtggatcccg
     2401 cgcggcgcga gttggacctg gccatcagcc aactgatcgg caaggccgga caccccagcc
     2461 cgtcggtcaa accgacaaag aagatcacca agaagggcaa gagcgaaaag cacgcccccc
     2521 gcggcggggc gacgaaacgg aaccagcgct ccaagcagcg cgggcgcaga gggcgatgac
     2581 gatcacggca agcgttaatg ctgggcgaga gcggcgacgc gtgctaaaac ttttagcaat
     2641 gagcggcatc actgaagttg agcgcgcggt tcgagaactt ccaccagcgg agttggctgc
     2701 gtttcgcgaa tggttcgcgc agtttgatgc ggcgatgtgg gatcgccaaa tcgaagcgga
     2761 cgtgtcggcc ggcagattag acgcgtttgc ggacgagatg ctgcatgacc tgcgcgacgg
     2821 ccgctgcaag gaactgtgaa tccc
//
LOCUS       DATIMH010000540         1718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3019074, whole genome shotgun sequence.
ACCESSION   DATIMH010000540 DATIMH010000000
VERSION     DATIMH010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3019074"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VLI90_09415"
     CDS             complement(<1..795)
                     /locus_tag="VLI90_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV14467.1"
                     /translation="MQYRQLGRSGLRVSPLCLGTMNFGPQTSEPDSFAIMDKAIDVGI
                     NFFDTADVYGWKQGEGYTEQIIGRWFAQGGGRRERVVLATKVYGDMAPPAPIPDPNFI
                     RGLSARKIIRSCDNSLRRLQTDWIDLYQMHHVDRSCPFDEIWQAYETLVRQGKVLYAG
                     SSNFAGWDIATANQSAQHRNFPGLVSEQSIYHLDNRMVELEVIPACRHYGLGLIPWSP
                     LGGGLLGGALQKRAEGRRGNEKFAQTVEKKRPQLEKYESLCRELGES"
     gene            complement(911..>1718)
                     /locus_tag="VLI90_09420"
     CDS             complement(911..>1718)
                     /locus_tag="VLI90_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSV14468.1"
                     /translation="LAENPRDSTSAFRVLQLIDGVGPAIAKRAVAHLASHNGDLKSLN
                     TFVAPPAAAGQWPALVAMLLQIAPDDLPLAEQVAAVRKFYAPVLERRYDQAEMRRRDI
                     EQLEQIAGNFRSRSAMLTDLTLDPPTSTQDLAGPPLREEDYLILSTIHSAKGCEWDCV
                     YVIHAADGNIPSDMATGDDAQIEEERRLFYVALTRARDFLEVVFPLKHYHKKWSTGDR
                     HTYAQLTRFIPPQISDAFERVSLAIDRPADAPANIECGQAIRNKIAAMWG"
BASE COUNT          299 a          539 c          547 g          333 t
ORIGIN      
        1 cgattcgccc agttcgcggc agagcgattc gtacttctcc agctgcggac gcttcttctc
       61 caccgtctgg gcgaacttct cgttcccgcg gcggccttcg gcgcgcttct gcagcgcgcc
      121 gccgagcaat ccaccgccga gcggactcca tgggatgagc ccaagcccgt aatgccggca
      181 ggcggggatc acttccagct cgaccattcg gttgtcgagg tgatagatgc tttgctcgct
      241 gaccagcccg gggaagttgc ggtgctgagc cgattgattc gccgtcgcga tgtcccagcc
      301 ggcgaagttg ctgctgccgg cgtacagcac cttgccctgc cggacgagcg tttcgtacgc
      361 ctgccagatt tcatcgaacg gacaagatcg atcgacgtgg tgcatctggt acaggtcgat
      421 ccagtccgtc tgcagccggc gaagcgagtt gtcgcaagag cggatgatct tgcgagcgga
      481 caaaccgcgg atgaaattgg gatcggggat cggcgccggc ggcgccatat ccccatagac
      541 tttggtggcg agcaccacgc gttcacgccg cccgccgccc tgggcgaacc accggccgat
      601 gatctgctcg gtgtagcctt cgccctgctt ccaaccatag acgtcggcgg tgtcgaagaa
      661 gttgatgccg acgtcgatcg ccttgtccat gatggcgaag ctgtcgggct cggaagtctg
      721 cgggccgaag ttcatcgtgc ccaggcaaag cgggctgacg cgaaggccgg accggccgag
      781 ttgacgatat tgcatgagga acacctcagt gggctgttcg tgattacgtg aaaccgaaca
      841 caagccataa tgcgtaacga tcaggcacgc aaccgatctt ttcacgtaat cacgtattca
      901 cgtattcact tcacccccac atcgcggcga tcttgttgcg gatcgcctgg ccgcattcga
      961 tgttcgcggg cgcatcggct ggacgatcga tcgccagact gacgcgctcg aacgcgtcgc
     1021 tgatctgcgg tgggatgaac cgcgtcagct gcgcgtaggt gtggcggtcg ccggtggacc
     1081 acttcttgtg atagtgcttc agcgggaaga cgacttccag aaaatcgcgc gcgcgtgtga
     1141 gcgcgacgta gaacagccgg cgttcctctt ctatctgtgc atcatcaccg gtggccatgt
     1201 ccgatggaat gttcccgtcg gcggcgtgga tcacgtacac gcaatcccac tcgcacccct
     1261 tggcggaatg gatcgtcgag aggatcaaat aatcctcctc gcgcagtggc gggccggcca
     1321 agtcctgcgt gctggtcggt ggatcgagcg tcaggtccgt cagcatcgcc gagcgcgagc
     1381 ggaagttgcc ggcgatctgt tccagctgct cgatgtcgcg ccgccgcatc tccgcctggt
     1441 cgtagcgccg ctccagcacc ggcgcgtaaa acttccgcac ggccgccact tgctcggcca
     1501 gcgggagatc atcgggtgcg atctgcagca gcatcgccac cagcgccggc cactgcccgg
     1561 ccgcagcggg tggcgcgacg aacgtattga gacttttcag atcgccgttg tgtgatgcca
     1621 gatgcgcgac ggcgcgcttg gcgatggccg gcccgacgcc gtcgatcagc tgcagcacgc
     1681 gaaaggcgct ggtgctgtcg cgcgggttct cggccagt
//
LOCUS       DATIMH010000541         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_130817, whole genome shotgun sequence.
ACCESSION   DATIMH010000541 DATIMH010000000
VERSION     DATIMH010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130817"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="VLI90_09425"
     CDS             complement(<1..1424)
                     /locus_tag="VLI90_09425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV14469.1"
                     /translation="MPDDPFSGYGLRPVHLADRDTFTAFFQSLRQPLSDYTFSQIYTW
                     RNSLHIVWGVLAGHLCVFANGSGDLTLLIPPIGDTNGDNALRAAFDLMNDYNSKQGVP
                     ERTRVEYASEELLARFDRARLNVVPMGTDYIYDTQRMIDLAGGDLASKRQAKNRFCRL
                     YEHRVETYGAAQHLEGCLALLERWRTHQDAQHLEEPNINAIKRQKETIATELALRSSA
                     ELGFKGLVVHVKDPATHEWSLRGFTFGEYLGADQSSIVIEKTDLEVKGLAQFIFSEFC
                     RRYFADRPLCNVGDDWGLESLAWTKMSYRPVKLLQKYAMTMARSTGISRASSAPAQVL
                     VRPATKDDLAATVELETACFASDLSLKKRQLQYLQQRSTAVFLVAESAGRVVGEGIAL
                     VRQHTGGRSGRIYSLAVDQSRRGEGVGQRLLHAMLDELAARGVKRVYLEVEQSNAAAI
                     ALYQRHGFRSIGTLPDYYGDGRHA"
     gene            complement(1607..>2033)
                     /locus_tag="VLI90_09430"
     CDS             complement(1607..>2033)
                     /locus_tag="VLI90_09430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV14470.1"
                     /translation="FLAAAPKGQHPGDTKRGLSPSYHFWMRLGDDAAPPVTIAGGIGL
                     RIGNTPNIVQYVGHFGYQVYPPARGHHLAERACRLLLPLARRHGLDELWITCNPDNIA
                     SRRTCERLGATLVNIVPVPKDHELYSRGDYQKCRYRLAL"
BASE COUNT          375 a          671 c          608 g          379 t
ORIGIN      
        1 atcgcgtggc ggccgtcgcc gtaataatcc ggcagcgtcc cgatcgaccg gaagccgtgc
       61 cgctggtaca gcgcgatcgc cgcggcgttg gattgctcga cctcgagata cacccgcttc
      121 accccgcgcg ccgccagctc gtcgagcatc gcgtgcagca agcgctggcc aactccttca
      181 ccgcggcgag actgatcgac ggccaaacta tagatccgcc ccgagcggcc acccgtgtgt
      241 tgccgcacca atgcaatgcc ttcccccacc acccgaccgg cggattcggc aaccaaaaac
      301 accgccgtcg atcgctgctg aagatactgc agctgccgct tcttcaggct caggtccgat
      361 gcgaagcagg cggtttccaa ttcgaccgtt gccgcaagat cgtccttcgt tgctgggcgg
      421 acgagtactt gcgccggcgc gctcgacgcg cgcgagatgc ccgtgctacg ggccatcgtc
      481 atcgcgtatt tctgcagcag cttcaccggg cgatagctca tcttcgtcca cgccaacgac
      541 tccagtcccc aatcgtcgcc cacgttgcac agcggccgat ccgcaaaata ccgccggcag
      601 aattcgctga agatgaactg cgccaggccc tttacctcga ggtccgtctt ttcgatgacg
      661 atgctgcttt gatcggcgcc gagatattcg ccgaaggtga agccgcgcag cgaccactcg
      721 tgcgtcgcgg ggtctttcac gtgaacgacg agtcccttaa agcccaactc cgccgacgac
      781 cgcagcgcca gttccgtcgc gatcgtctcc ttctgccgct tgatcgcgtt gatgttcggc
      841 tcttccagat gctgtgcatc ctgatgcgtt cgccaacgct ccagaagcgc caggcaaccc
      901 tcgagatgtt gagccgcgcc atatgtttcg acgcgatgct cgtacagccg gcagaagcgg
      961 ttcttcgcct gccgtttgct cgccagatcc ccgccggcga ggtcgatcat gcgctgcgtg
     1021 tcgtagatgt agtcggtgcc catcggcaca acgttcaagc gcgcgcgatc aaagcgcgcc
     1081 agtaactctt cgctggcgta ctcgacgcgc gtgcgctccg gcacgccctg cttcgagttg
     1141 taatcattca tcaaatcgaa cgccgcgcgc agcgcattgt cgccattggt atcaccgatc
     1201 ggcgggatca gcagcgtcag gtcgccgctg ccgttggcga agacgcacag gtgcccggcg
     1261 aggacacccc acacgatgtg caggctgttt cgccaggtgt aaatctgcga gaaggtgtag
     1321 tcggagagcg gctggcgaag ggactgaaaa aacgcggtga acgtgtcgcg gtcggcgagg
     1381 tgcaccggcc gaaggccgta gcccgaaaag ggatcatctg gcataaaagc gacggtttcc
     1441 tccggccgga tcggcgcgcg cagctgatcc gttgctcgtt atatcggtcg attcgaaggg
     1501 agatgttcat aaagccccga ctctgccggg ggaggttttc gaatttgaac gcgcgatcga
     1561 tttctcgaac atgcacccgg gcagggcccg gggcttttcc ggggctttag agcgccaacc
     1621 gataccggca cttctgataa tccccgcgcg agtacagctc gtggtccttc ggcaccggca
     1681 cgatgttcac gagcgtcgcc cccagccgct cgcaggtgcg gcgggacgcg atattatcgg
     1741 ggttacacgt gatccacagc tcatccaacc catgccgccg ggccagcggc aacagcaatc
     1801 ggcacgcgcg ctcggccagg tgatgcccgc gcgccggcgg gtagacctgg tatccaaagt
     1861 gaccgacgta ctgcacgatg ttcggcgtgt tgccgatgcg cagcccgatg ccgccggcga
     1921 tcgtcacggg tggcgccgca tcatcgccca aacgcatcca gaaatgatag ctcggcgaca
     1981 agccgcgttt cgtgtcacct ggatgttgcc ccttcggggc cgccgctaaa aag
//
LOCUS       DATIMH010000542         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3306150, whole genome shotgun sequence.
ACCESSION   DATIMH010000542 DATIMH010000000
VERSION     DATIMH010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3306150"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VLI90_09435"
     CDS             <1..254
                     /locus_tag="VLI90_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14471.1"
                     /translation="TSVSTNGPLNAGTPIQVARGNGAFNGTTDWNADTLGNAGNIRFR
                     HSSNRQMNALMMDGHVTSFKYNPLTHQSDMLEGNINAIP"
     gene            384..1757
                     /locus_tag="VLI90_09440"
     CDS             384..1757
                     /locus_tag="VLI90_09440"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HSV14472.1"
                     /translation="MHSAVQDETGESASQLAEKDAKIELRTGLPANHTFTAAEDEFLD
                     DLERRGIQFYIDEADPQTGLMPDRAKADGSHAGDVASIASVGFGLTALCIGDERGWVP
                     HQDAYDRSLRVLRFLRDRAPQDHGHFYHFLDMRTGKRVWNCEVSNIDTALLMAGVLTV
                     RQHYPNSELASLANELFERVDWPWLLGSSAGERDPGHAMLLNMGWKPESGFLRAQWGQ
                     FSEGPLIYLLGLGSHTHPLPPESWRAWQRKPIESYAGLTFIQCPPLFTHQYPQAWFDL
                     RGQHDDAADYFRNAQLATLAQRQWCIDELSKRFPTYGQNMWGLTASDSEHGYTAWGGP
                     PQQGEIDGSVVPAAAAGSLAFEPRLCLDALQAMHARYGEKGYLKFGFVDAFNPATGWY
                     NPDVLGIDIGPSVLMAENCRSGFVWRTFMSSPEAQAALRAAGFQANGSQAQGSGTSSL
                     LKDEQNN"
     gene            1822..2718
                     /locus_tag="VLI90_09445"
     CDS             1822..2718
                     /locus_tag="VLI90_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV14473.1"
                     /translation="MRGFSGFKAAFVGSAALLFSAASLYAQPVPPVPPQPTNGLTVIP
                     RFFNDFPNSTQTYSINGGPTITNPPANVGIPVAGAVNSVTVNDTNMVNSPASGFVNRD
                     DLMLSTDAGATPFVGSINTPFFQTATINLTDLTDTQRKEAGIRVKAPVTGDAVFEINT
                     TGEIVAFGGGAPFFNFRPNIEAAYIPGQTITLSEQYTPGVGGTTNGNPGTMQYWAQLS
                     GGPLLTSGPLAWSNLEGGPGPNSFNIGAFVQAPDGSGASPSDFVNAAFTNISGAVPEP
                     ASLGTIGFGALMLLGRRRQRVA"
     gene            2959..>3403
                     /locus_tag="VLI90_09450"
     CDS             2959..>3403
                     /locus_tag="VLI90_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14474.1"
                     /translation="MRSQRHRAVPTSASTQIEALETRCLLSAISPTFIAGAIANINSA
                     QNAGANGIAFTDQDIGSPAVAGSSSYSNGTYSVTAAGTGIGGNGDQFNFAAVHFTGDG
                     SVVARLASLVNTSTSAQAGIMIRSGTAANAAVAALLVTPSNQLQFI"
BASE COUNT          659 a         1117 c         1022 g          605 t
ORIGIN      
        1 ctaccagcgt atcaaccaat ggaccactaa atgcgggaac gcccattcaa gtggctcggg
       61 gcaatggagc tttcaatgga acgacagact ggaacgcgga cacattgggc aatgctggca
      121 acattcgctt ccggcactcc agtaacaggc aaatgaacgc actgatgatg gacggtcacg
      181 tcacgtcgtt taagtacaac ccgttgacgc atcagagcga catgctcgaa ggaaacataa
      241 acgctatccc gtagaaccga ccgtgcgaga gggtgagagt gcatttctca agaactagat
      301 tcggccttgg cagcgctgca gcctcacggt tggcgggctg tattgccatt ctcgttaccg
      361 caatctccct cggacatggg tatgtgcact ccgccgtcca ggatgaaacg ggcgaatccg
      421 ccagccagct tgcggaaaaa gatgcgaaga tcgagcttcg aactggcctt ccggcgaatc
      481 acacgtttac ggcggccgaa gacgaattcc tcgacgacct cgaacgccgc ggcattcagt
      541 tctacattga cgaagccgac ccgcagactg gcctgatgcc ggaccgggcc aaggcggacg
      601 gctcgcacgc cggtgatgtg gccagcatcg cttcggtcgg tttcggtttg acggcgttgt
      661 gcatcggcga tgaacgtgga tgggtgccgc accaggatgc gtacgaccgc tcgctgcgcg
      721 tgctgcggtt cctgcgcgat cgcgcacccc aggatcacgg gcacttctat cacttcctgg
      781 acatgcgcac cggtaagcgc gtgtggaact gtgaagtctc caacatcgac acagcgctct
      841 tgatggccgg cgtgctcacc gtccgccagc attatccgaa ttcggaacta gcgtcgctgg
      901 cgaacgaact attcgagcgc gtcgattggc cgtggctcct gggatccagc gccggcgagc
      961 gcgaccccgg gcacgcgatg ttgctcaata tgggatggaa gcccgaaagc ggcttccttc
     1021 gcgcgcagtg gggacagttc agtgaaggcc cgctcattta cctgctgggc ctcggctcgc
     1081 acacgcaccc gctgccgccg gaatcgtggc gcgcgtggca gcgcaagccg atcgaatcgt
     1141 acgcgggatt gacgttcatc caatgcccgc cgctgttcac gcatcagtat ccgcaagcgt
     1201 ggtttgacct gcgcggccag cacgacgacg ccgccgacta cttccgcaac gcccaactgg
     1261 ccacgctggc gcagcggcag tggtgcatcg atgagctgag caagcgattc ccgacgtatg
     1321 ggcaaaacat gtggggcctc accgcgtccg acagcgagca tggttacacc gcctggggtg
     1381 gcccgccgca gcagggcgag atcgacggca gcgtcgttcc cgccgccgcc gccggcagtt
     1441 tggcgtttga acctcgactg tgcctcgacg cgctgcaagc gatgcacgcg cgttatggcg
     1501 agaagggcta tctgaagttc ggcttcgtcg atgcgttcaa tcccgcgacc ggctggtaca
     1561 atccggacgt gctgggcatc gacatcgggc cgtcggtgct gatggccgag aactgccgca
     1621 gcgggtttgt gtggcggaca ttcatgtcga gccccgaggc gcaggccgcg ctgcgcgccg
     1681 cgggcttcca ggcaaacggt tcgcaagcgc aaggttcggg gacaagttct ctgttgaaag
     1741 acgaacaaaa taactgaggg cgctcgaata gcgttattcg ggcccgtctg taggcgtagg
     1801 tcttattgtg aggaggttag aatgagaggc ttcagtggat ttaaggctgc cttcgttggc
     1861 agcgctgcgt tgctgttctc ggccgcatcg ttgtacgcgc aaccggtccc accggtcccg
     1921 ccgcaaccga ccaacgggct gacggtcatc ccgcgattct tcaacgattt cccgaactcg
     1981 acgcagacgt actcgatcaa cggcggcccc acgatcacca acccgccggc caacgtggga
     2041 atccccgttg ccggggccgt taattccgtg acggtgaatg acacgaacat ggtgaactcg
     2101 cccgcgtcgg gctttgtgaa ccgtgacgat ctgatgctct cgacggacgc gggcgctacg
     2161 ccgttcgtcg gcagcatcaa cacgccgttc ttccagaccg caacgatcaa cttgaccgac
     2221 ctgaccgaca ctcagcgcaa ggaagcgggt attcgcgtca aggcgccggt gaccggcgat
     2281 gccgtcttcg aaatcaacac caccggtgaa atcgtcgcct tcggcggcgg ggcgccgttc
     2341 tttaatttcc gcccgaacat cgaggcggcg tatatcccgg gccagacaat cacgctctcc
     2401 gagcagtaca ccccgggggt gggcggcacg accaacggca acccaggaac gatgcagtat
     2461 tgggcgcagc tgtcgggtgg cccgctgctg acctccggac cactggcttg gagcaatctc
     2521 gaaggcggtc ccggcccgaa cagcttcaac atcggcgcct tcgtgcaggc ccccgacggc
     2581 agcggcgctt caccgagcga ttttgtcaac gcggcgttca ccaatatctc cggcgcggtc
     2641 cccgaaccgg cgagcttggg cacgatcggt ttcggcgccc tgatgctgct cggccgccgt
     2701 cgccaacgcg tggcgtgaat cgcgcgcatc cgcgcgcaat gcggtcccgc tgcgagggaa
     2761 tgaactgccg cagaggggaa gcgatccaga ggccccgccc gtgcgtacca cacgcacgcg
     2821 cggcacctcc aggtcgcgct ccccctgcgg cccccttccc agcgccactg tgtaagtaga
     2881 actccaagat cctcgccccg ttttagcggc ggcccgcagg gccgagcgtt tcgttggttt
     2941 tggtttcaga cccgcaacgt gcgttcacaa cgtcacagag cagttcccac ctccgcttca
     3001 acccaaattg aagccctcga aacccgttgc ctgctctcgg cgatcagccc aacgttcatc
     3061 gccggcgcaa ttgccaacat caactccgcc caaaacgccg gcgccaacgg aatcgccttc
     3121 accgaccagg acatcggctc ccccgcggtt gccgggtcca gctcgtattc caacggcacc
     3181 tactccgtca ccgccgccgg aaccggcatc ggtggcaacg gcgaccaatt taacttcgcc
     3241 gccgtccact tcaccggcga cggcagcgtg gtcgcccgcc tcgcgtcgct ggtcaacacc
     3301 agcacgtccg cccaagccgg catcatgatc cgcagcggca cggcagcgaa cgcggcggtc
     3361 gccgcgctgc tggtgacgcc atcaaaccaa ctgcaattca tca
//
LOCUS       DATIMH010000543         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2150841, whole genome shotgun sequence.
ACCESSION   DATIMH010000543 DATIMH010000000
VERSION     DATIMH010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2150841"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VLI90_09455"
     CDS             complement(<1..526)
                     /locus_tag="VLI90_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14475.1"
                     /translation="MSNLAGEDDGVAHATDAVDLGGGVLCRRATPPDVLPALRMILGA
                     SGHLAEEAQAADFMRITTRRGVQLSDIHVADTREGLIWAVLPMLSPGRTMLLMGASVH
                     FAGEYLPAVRQVIENVCQKFADRGVQLAQALLDPADPHTLQSYMSAGFERMAELIYLH
                     RTLRRAPAPPPLPPA"
     gene            691..1311
                     /locus_tag="VLI90_09460"
     CDS             691..1311
                     /locus_tag="VLI90_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14476.1"
                     /translation="MLLGTSPARADFPKPSPYPISWELKFTHGLPKRIVVNVPNSNIP
                     QAYWYMTYTVTNLGDKEQMFLPRFELMTEDGRLTRGDVNIPRDVFDAIKAKERNKLLE
                     PVTSITGLLRLGEAEARDGVAIFPEPTPRMGHFSIFVTGLSGEAVTLKNVNGEFQKID
                     DAEQMKNPKDLIILRKTLQLNFFIRGDEVYPGEDEVNQDTEVWIMR"
     gene            complement(1375..>1655)
                     /locus_tag="VLI90_09465"
     CDS             complement(1375..>1655)
                     /locus_tag="VLI90_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14477.1"
                     /translation="RRLDVAILQRYLLDEIIQPNFTSGKEIVKGYTADATEIVPKTDG
                     TKFQIALLLKSTPLGALEQLAVHNEVMPQKSTYFYPKLATGMLINPVG"
BASE COUNT          315 a          512 c          521 g          307 t
ORIGIN      
        1 acgccggcgg cagcggcggg ggcgcggggg cgcggcggag cgtgcggtgc aggtagatca
       61 actccgccat gcgctcaaag cctgcgctca tgtaggactg aagcgtgtga ggatcggccg
      121 ggtcgagcag cgcctgagcc agctgcactc cgcgatcggc aaacttctgg cacacgtttt
      181 cgatgacctg ccggaccgcc ggcagatatt cgccggcgaa gtggacggat gcgcccatca
      241 gcagcatcgt tcgcccgggg ctcagcatcg gcagcaccgc ccagatcaac ccttcgcgcg
      301 tgtcggcgac gtggatgtcg gacagttgca cgccgcggcg cgttgtgatg cgcatgaagt
      361 cggcggcctg cgcttcctcg gccaagtgtc cgctcgcgcc caggatcatg cgaagcgccg
      421 gcaggacatc tggcggtgta gcgcggcggc agagaacgcc gccgccgaga tccaccgcgt
      481 cggtcgcatg ggcgacaccg tcgtcttcac cggctaaatt tgacactccg ttcactgacc
      541 gtaagaatac atggcgtcgt gcgtgcggca atcgcaaatt cgccgcgttc cgcttgttgc
      601 gacgcgcgcg cagacgcgcg gagaatcaca cctatgtccc ggccgcgaag ctcctctgtc
      661 gttacatcgt tgatcattgg cgctctgttg ttgctgctag gcacatcgcc ggcgcgcgcc
      721 gatttcccca agcccagccc gtatccgatc agctgggaac tgaagttcac gcacggcttg
      781 ccgaagcgca tcgtggtcaa cgtgccgaat tcgaacatcc cgcaggcata ttggtacatg
      841 acgtacaccg tcacgaacct cggcgacaag gagcagatgt tcctgccgcg gttcgagctg
      901 atgaccgaag acggccggct gactcgcggc gacgtgaaca tcccgcgcga cgtgttcgac
      961 gccatcaagg cgaaggagcg caacaagctg ctggaaccgg tgacgtcgat caccggattg
     1021 ctgcgcctgg gcgaagccga agcgcgcgac ggcgttgcga tctttcccga gccgacgccg
     1081 cggatggggc atttctcgat cttcgtgacg ggcctgagcg gcgaagcggt gacgctgaag
     1141 aacgtgaatg gcgaatttca gaagatcgac gacgccgagc agatgaaaaa tcccaaggac
     1201 ctgatcatcc ttcgcaagac gctgcagctg aacttcttca tccgcgggga cgaggtgtat
     1261 cccggggagg atgaagtgaa tcaggatacg gaagtttgga tcatgcgctg aagccgcttg
     1321 cggcttagca tctttttccg cccgggtgcg aacaaatgct aagccgcaag cggctcatcc
     1381 caccggattg atcaacatcc ccgtcgcgag cttcggataa aaatacgtgc tcttctgcgg
     1441 catcacttcg ttgtgaaccg ccaactgctc cagcgccccc agcggcgtgg acttcagcag
     1501 cagcgcgatc tggaatttcg tcccatccgt ctttggcacg atctccgtcg catcggcggt
     1561 gtatcctttg acgatctctt tcccgctggt gaagttcggc tggatgatct cgtcgagcag
     1621 gtagcgctgc aggatcgcca cgtcgaggcg gcgcc
//
LOCUS       DATIMH010000544         4224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4171948, whole genome shotgun sequence.
ACCESSION   DATIMH010000544 DATIMH010000000
VERSION     DATIMH010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4171948"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..414
                     /locus_tag="VLI90_09470"
     CDS             175..414
                     /locus_tag="VLI90_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14478.1"
                     /translation="MSLSRPRLTQLILDQLGQREMRLLALVVAIRKNLDRSEAVKGDL
                     SEIVKSVLRKLVAAEQVVDIEGTYALASRKLVKSR"
     gene            610..1149
                     /locus_tag="VLI90_09475"
     CDS             610..1149
                     /locus_tag="VLI90_09475"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HSV14479.1"
                     /translation="MLRRHLIPAAVLCVAALAVGCEQDISGLGGKASPTLPIGESHFL
                     KVLGNENQQEVESAKIALQRSRNEQVRQYAQQEIDDHSQSQNQLQQLAQNKGAELPKV
                     LGNMEQGMVNNLSAQSDSNFDKTYIDNEVTAHQDAVNNVNAEAMNGADVDLKAYAQKI
                     LPTLQKHLIAAKQLQGTAK"
     gene            complement(1354..1575)
                     /locus_tag="VLI90_09480"
     CDS             complement(1354..1575)
                     /locus_tag="VLI90_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14480.1"
                     /translation="MDADLVNAAVGLQQASTMSRIQFAVARKILDAQNADGNAALQLL
                     NAATGGVTKAGDAMVAAATGLGGQLDVTG"
     gene            complement(1656..2894)
                     /locus_tag="VLI90_09485"
     CDS             complement(1656..2894)
                     /locus_tag="VLI90_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSV14481.1"
                     /translation="MLRLIHFVLIAAYIAAPVIFLSRGIYLAARRGKRTDLQRFWLTA
                     FGGLLSAVCVCGVFAVTIHGRVMLTQLLLTAYFATALLLLLKSLDRLLRLSLSSAMML
                     RRRPGPAWSYGLRVSFAMLMRTAVLFCIGVPYVLATVLTYRPKVSQSDNPQSMFHWSY
                     QPVSFRAIDGTRLAGWWIPAAGGASPRTVIFCHGLSATKATQLAMTRRLVPGGYNVLA
                     FDFRAHGDSGGQLITFGDAERYDVLGAVRWLRQNHANACKRLMGLGASTGGAALIAAA
                     ADPGPEGQNIDAIAVCGTFDRLDETVADVCHDYVPTPLKWLTLHVGLPLASAQVGYDL
                     KNFAPAEAVKLLWPRPILVIHGVDDQMVQFERGQALFDAALQPKYSDWVDRAFHEDVI
                     KREDTARLLKRYFDSAVSVL"
     gene            complement(3193..3468)
                     /locus_tag="VLI90_09490"
     CDS             complement(3193..3468)
                     /locus_tag="VLI90_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14482.1"
                     /translation="MPRQPTKRSNSRAKSDIALPGEKVVRETKREVGVKPVKAGPDAK
                     RPTPGVALDRRREVAPSGPAEVKRSEANPVEPIARWGARTRPRRPTA"
     gene            3517..4167
                     /locus_tag="VLI90_09495"
     CDS             3517..4167
                     /locus_tag="VLI90_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSV14483.1"
                     /translation="MMERRKCGVALPVLHAAITIRGLVSQNTPSTQPLSRAIDEWSQL
                     LPKMAAFEPMLHRLGETLLACWAARGKVLTAGNGGSAADAIHLAEELVVRFHKPRRAL
                     AAIALCDAGNLTCAANDMGFDEVFARQVEALGNPGDVLVVFTTSGNSPNIMRAIETAK
                     MAGLKTVAFIGKTGGKAKAHCDFEFLVPSQNTARVQEAHLMLYHSLCEWIDQRVGE"
BASE COUNT          840 a         1360 c         1315 g          709 t
ORIGIN      
        1 catggcctgc gctggtggga gatcgacttc agctactaca tcatccgcgc gatgtcgctg
       61 gtcggcctgg cgtggaagat tcgcgtgccc gaacatgacg cgattcaggc caaactgaaa
      121 tagtcccgcg aaaaatcccc gctgagcgcg cgtttcggtt acgatggccc gtctatgtca
      181 ctctcacgac cccgcctcac gcaactcatc ctcgatcaac tcggacaacg cgagatgcgt
      241 ttgctggcgc tggtcgtcgc catccgcaag aacctcgatc gctccgaagc ggtcaaaggc
      301 gacttgtcgg aaatcgtgaa gtcagtcctt cgcaaactgg tcgccgcgga acaggtcgtg
      361 gacatcgaag gcacctacgc actggcatcg cgcaagctgg taaagagccg gtgactttaa
      421 atggcgggcg cacccgctcc tcgcaccccg ccgtttgatc ctccttgtac ggcggccgct
      481 aaacattccg cacaccccac ccacggttga ccccgattcg gcacgttggt tgcttaacca
      541 cgcgaagtga ccgcgtgtcg caccgtgcga tgcgggtgga acggattcat tagcgaaacg
      601 gagcgtccta tgttacgacg acatttaata cccgccgccg tgctgtgtgt cgcagcgctg
      661 gccgtcggat gcgagcaaga catcagcgga ctcggcggca aggcgagccc gaccctgccg
      721 atcggtgaaa gccatttcct gaaagtcctt gggaacgaaa accagcagga agtggagtcg
      781 gcgaagatcg cgctgcagcg cagccggaac gaacaggtgc gacaatatgc gcagcaggag
      841 atcgacgacc actcgcaatc gcagaatcag ctgcagcagc tggcccagaa caagggcgcg
      901 gaattgccga aggtgctggg caacatggaa cagggcatgg tcaacaacct gtccgcccag
      961 agcgacagca acttcgacaa gacgtacatc gacaacgaag tgacggccca tcaggacgcc
     1021 gtcaacaacg tcaacgccga ggcgatgaac ggcgccgacg tggacctgaa ggcgtatgcg
     1081 cagaagatcc tgccgacgct gcaaaagcac ctgatcgcgg ccaagcaact gcaaggcacc
     1141 gccaagtaag cagaaatcac gagttagaat ttgcggttga aacagaaggc cggaattgca
     1201 cgcgtttacg cgtgtggttt cggctttcgc ttttgaatgt ggaccccttt gcgcgcgcaa
     1261 cgttcttcga agttcggagc gtccgcgggc taaagcctcg cggctgcgaa gtcgcagtgt
     1321 gtatttgaac tcagacgagc gaaatgagcg ggatcagcca gtgacgtcga gttgtccgcc
     1381 aagacccgtc gcggcggcga ccatcgcgtc gcccgccttg gtcaccccgc cggtggcggc
     1441 gttcagcagc tgcagcgccg cgttcccgtc cgcgttctgc gcgtccagga tcttacgagc
     1501 caccgcgaac tgaatgcggc tcatcgtgct cgcctgctgc aatcccaccg cggcgttgac
     1561 caggtctgcg tccatccgtg ttgattacat cggcacgatc cggatgtgga cttggacgat
     1621 cgagacgtcg gcgtccgata acttgcgctt tcccctcaca gcacgctcac cgccgaatcg
     1681 aagtatcgct tcagcagccg cgccgtgtcc tcacgcttga tgacatcttc gtggaacgct
     1741 cgatccaccc agtcgctgta cttcggctgc agcgccgcgt cgaaaagcgc ctgcccgcgt
     1801 tcgaattgga ccatctggtc atccacgcca tggatcacga ggatcgggcg cggccagagc
     1861 aacttgacgg cttccgcagg cgcgaagttc ttcaagtcgt atcccacctg cgcgctcgcc
     1921 aacggcaggc cgacgtgcag cgtcagccac ttcagcggcg tgggcacata gtcgtggcag
     1981 acatcggcga ccgtttcatc gagccgatcg aacgtcccgc agacggcgat cgcgtcgatg
     2041 ttctgcccct ccggtcccgg gtcagcggcg gcggcgatca acgccgcccc gccggtgctg
     2101 gcgccgagac ccatcaaccg cttgcacgcg ttggcatgat tttgacgcag ccaacgaacg
     2161 gcgccgagga cgtcgtatcg ttcggcatcg ccgaaggtga tgagctgccc accgctgtcg
     2221 ccgtgggcgc ggaaatcgaa cgccagcacg ttgtacccgc cgggcacgag ccggcgggtc
     2281 atcgccagct gcgtcgcctt cgtcgcggac agtccatgac agaagataac ggtgcgcggc
     2341 gacgccccac cggccgcggg aatccaccag ccggccaggc gcgtgccgtc gatcgcacga
     2401 aaactcaccg gctggtacga ccaatgaaac atcgactgcg ggttgtctga ctggctcacc
     2461 ttcggtcgat acgtcagcac ggtcgcgagc acatacggca ccccgatgca gaacagcacc
     2521 gcggtgcgca tcagcatcgc gaagctgacg cgcagcccat acgaccaagc cggccccggc
     2581 cggcggcgga gcatcatcgc gctcgacaac gacaagcgca ggagccgatc gaggctcttg
     2641 agcagcagca acagcgccgt ggcgaagtac gcggtgagca gcagttgcgt gagcatgacc
     2701 cggccgtgaa tggtgacggc gaacacgccg cagacgcaga cggcggagag cagcccgcca
     2761 aaggcggtga gccagaagcg ctggagatcc gtccgcttgc cgcggcgcgc cgcgaggtag
     2821 atgccgcgcg acagaaaaat gaccggcgcg gcgatgtagg cggcgatcaa gacaaagtga
     2881 ataagacgca gcatgcggcg gcgcaatgat agccgcgcag atgtcccggg gcatagcccc
     2941 gggctttcgg gaaagatctg tagccagggc aaacgcatgt aggaactgcg ccctcttgtc
     3001 ccctctcccg gtacgccggg agagggctag ggtgagggtg atttcgaatg ccagcggcta
     3061 tcgaaattca aaatcgccct cgcccctgcc ctctcccgga gacctccggt cagcagtacc
     3121 gggagaggga gctatatgcg attgccctga tctctagcgc ccggcgcgcg cacgcgcaac
     3181 aagagcccaa ggctaagccg tgggacgacg gggccgggtc cgcgcgcccc atcgcgcaat
     3241 cggttccacc ggatttgctt cgctgcgctt cacctccgcc ggtccgctcg gcgccacctc
     3301 gcggcggcga tccagcgcca cgccgggcgt cggccgcttc gcatctggac cggctttgac
     3361 gggcttaacg cccacctcgc gcttggtttc gcgaaccacc ttctcgcccg gcaacgcgat
     3421 gtcactcttg gctcggctat tggaacgctt ggtcggctgt ctgggcatgt gcgggaacct
     3481 cctgttcggt cgctcaagaa tagcgcgacg caggtcatga tggagcgccg aaagtgcggg
     3541 gtcgccttgc cggtgttaca cgccgcgatt acgatccgcg gactggtgtc gcagaacacg
     3601 ccgagcactc aacccctctc gcgagcgatc gatgaatggt cgcagctgct gccgaagatg
     3661 gcagcgttcg aacccatgct tcatcgcctg ggtgaaacgc tgctggcctg ctgggccgcg
     3721 cgcggcaaag tgctgaccgc cggcaatggc gggagcgccg ccgacgcgat ccacctggcc
     3781 gaagaactgg tcgttcgctt ccacaaaccc cgccgggcgc tggcggccat cgcactgtgc
     3841 gacgccggga acctcacctg cgccgccaac gacatgggct tcgacgaagt cttcgcccgg
     3901 caggtcgaag cgctgggcaa ccccggcgac gttctggtcg tcttcaccac cagcggcaac
     3961 agcccgaaca tcatgcgcgc aatcgaaacc gcgaagatgg cgggcctgaa gacggtcgcc
     4021 ttcatcggca agaccggcgg gaaggcgaag gcacattgcg attttgaatt cctcgttcca
     4081 tcgcaaaata ccgcgcgggt gcaggaggcg cacctgatgc tgtatcactc actgtgcgag
     4141 tggatcgatc agcgagtcgg ggagtaggga tggttcgcgc aatgggatgg cggcgtcttc
     4201 gcgttcttct tccggcgctt ttgg
//
LOCUS       DATIMH010000545         6489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_131881, whole genome shotgun sequence.
ACCESSION   DATIMH010000545 DATIMH010000000
VERSION     DATIMH010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131881"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1139)
                     /locus_tag="VLI90_09500"
     CDS             complement(27..1139)
                     /locus_tag="VLI90_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV14484.1"
                     /translation="MSKPVRVGVVGCGAISGAYLGMAKNFPNVEMFACADLDPARAAA
                     AAEKFGVANVLSVEQLLAHPDIEMVLNLTIPKAHAPVALQALATGKHTYMEKPLGVNR
                     EEGAKVIDAARAKNLRVGCAPDTFMGSGIQTARKVIDDGVIGRPVAFTALMQGRGHEH
                     WHPSPEFYYEPGGGPMFDMGPYYLTALLNLLGPVKRISGMSTIAIPERTITSQPKAGK
                     KITVQTPDHIVGLMEFTNGAVGTIIQSFATVEGGHDGSHPIIINGTDGAVRVPDPNTF
                     DGTVIVRRHGEKDSIEVPSQFHKGYGRSVGLADMAVALRSGRAHRANGEQALAVLDLM
                     QGFLDSSTSGRMYEPRYAYERPAAMRADLPFGQLEP"
     gene            complement(1217..1831)
                     /locus_tag="VLI90_09505"
     CDS             complement(1217..1831)
                     /locus_tag="VLI90_09505"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HSV14485.1"
                     /translation="MDETLTLQFDGGSRGNPGPAGIGVVVAAADGTPLITLGRFIGRA
                     TNNVAEYRALITAMEEARKLGAKKILIRGDSELIIKQMRGEYRVKNPDMKALYDEAQK
                     LIREFDQSKIEHNLRHKNELADQLANLAMDRRADVTEIPGAGGAAEAKPSPLDEPSPV
                     APKVGDRIACPRCGCSIELSTQSRLRPHQLKPFTCQCGAKMHQP"
     gene            complement(1824..3569)
                     /locus_tag="VLI90_09510"
     CDS             complement(1824..3569)
                     /locus_tag="VLI90_09510"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSV14486.1"
                     /translation="MLLARLKNPGRRSTSEPGVFSAARQSLYKSPACPDILASVVVFP
                     MKRFALVVSALLLMLAQPASAAVRDVLARASNDRLWVARVVVENTPRGPVEQTIILVR
                     PSGPGQQWKTLAVVPTRVTALASRSSQLVVLLSNGSWQSIWAPDGAATGQPLPANAQI
                     RALGDDGETLWAVGLVKGGMPAATRAAATQPTTLPAAAAEPGALADADSLVLFQQQSG
                     DWAPVAELPPDASLPLVGPTSDISLAIVAGRPYVAVGLTDGRIRTLMLQADGRWHGIG
                     VITPETDETFVSFDLLDTENHPVLWVGSGKGAGRIYLDASEQRAIPMKWVGENAPDST
                     PAIAAGGGYLRLIALHGDKLYEQRYDLDGKAVGAAAELAVPATVSGSAVEYWINGVLI
                     ALLAFSVMATLYRRTLLRKRGGELNPPPPAPLALRAGAGLVDLLPLIAGVIVVSVHTD
                     PSRDPRERLEDLPALITLGVAIAVYILHTTLVEVLTGRTVGKWLFGLTVVSVDGSPAG
                     RGQILIRNVLRALDVLLFPLFLVLVSPLRQRSADIAAGTVVVRLEDVRKQAAATTDGP
                     IDSAGPGPGGGTPNG"
     gene            complement(3762..4709)
                     /locus_tag="VLI90_09515"
     CDS             complement(3762..4709)
                     /locus_tag="VLI90_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14487.1"
                     /translation="MNVEREMHVTHRSSFIVHRLLLFAAALLPACRRPEPSPAKVYTG
                     PTQPMVEVVQAINANNIKVPSIWAQLEYNATIVDKERGTSNVVSGDGSLLYRRPMSLL
                     LRGQKDVAGTIFTIGSNDTEFWMIVTAGADTTWWGHYANIEKPCCRKIPVRPDLVLAV
                     LGVGLFDVDFLESPVPVMRFNNDADCYMFVWNIKRSDRWTAQKEIWFDRATKQPRRVY
                     LFDENGRVILRAYLRNHGPLEVPGMPRDQWPQVARHYELYFPDEGSRMTFDFTNAATE
                     HNGSPRPAAFHRPEEPGTKEVIQIDRDCADNITSAAQVH"
     gene            complement(4735..5286)
                     /locus_tag="VLI90_09520"
     CDS             complement(4735..5286)
                     /locus_tag="VLI90_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14488.1"
                     /translation="MSSTTAAATAHPAENKPAAKPGQYEVGRPSGRCCVCNAELPAGT
                     RIMAALRETPQGLERLDVSLDCWLAFNREDLLAFWQTVIPQHEAKKKLFVDDEVLCEL
                     FERLKDTAEPAKLNFRFVLGLILMRKRMLVYESSREENGQDIWTMRFRGKEERMDLVN
                     PRLDEQQVTDVSQQLGQILNEEL"
     gene            complement(5497..>6489)
                     /locus_tag="VLI90_09525"
     CDS             complement(5497..>6489)
                     /locus_tag="VLI90_09525"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HSV14489.1"
                     /translation="TVSFVVPDQNNDMHDGTIAQADGWLKTNIDPYVTWAKAHNSLLV
                     VTWDEDNSASNNRIPTILSGANLKNGTINNTTYTLHNLLRTIGDNFNAAPSGSAVNVK
                     DITGVFTTDAAQSIASFQQGVNGYAAAHDTEIRQDSTSSTFGSTTPLVVDQDDNTATG
                     NQPVQALVRFDNIIGSGAGQVPAGAKILSAKLNIHTGSTTNDNTGGSVELHRMLSSWD
                     ENSTWGGSFGGNGVDANNVDASSSADFTLIPNELDNAALFDVTDTVQAWTNGATNFGW
                     SLLANSTDGWRFDSSEFGTLADRPSLQITYAVPEPSAIGLAITGILLLGRRRGR"
BASE COUNT         1106 a         2080 c         2070 g         1233 t
ORIGIN      
        1 gaaatcctgt caaccatcgc agcacctcac ggctcaagct gaccaaacgg cagatccgcc
       61 cgcatcgcgg ccgggcgttc gtacgcatac cgcggctcgt acattctgcc gctggttgac
      121 gaatcgagga acccctgcat caggtccagc accgccagcg cctgctcgcc attcgcccga
      181 tgcgctcggc ccgaacgcag cgcgacggcc atgtcggcca ggccgaccga tcgaccatag
      241 cccttgtgaa actgactcgg aacctcgatc gaatctttct ccccatgccg gcggacaatc
      301 actgtgccgt cgaaggtgtt gggatccggc acacgcacgg cgccatcggt gccgttgatg
      361 atgatcgggt gcgaaccatc gtggccgcct tcgacggtcg caaaactctg gatgatcgtg
      421 ccgaccgcgc cgttggtgaa ctccatcaag ccaacaatgt gatctggcgt ctggaccgta
      481 atcttcttgc cagccttggg ctggctggtg atcgtgcgct cgggaatcgc gatcgtcgac
      541 atcccgctga tccgcttcac cggcccgagt aagttcaaca gcgccgttaa atagtacggc
      601 cccatgtcga acatcggccc gccgcccggc tcgtaataaa actccggcga cggatgccaa
      661 tgctcgtgcc cgcggccctg catcaacgcg gtgaacgcga ccggccgacc gatgacgccg
      721 tcgtcgatca ccttccgcgc ggtctgaatg ccggagccca tgaacgtatc gggcgcgcaa
      781 ccgacgcgaa ggttcttggc gcgggcggcg tcgatcacct tcgccccctc ctcacggttc
      841 acaccgagcg gcttctccat gtacgtgtgc ttcccggtcg ccaatgcttg cagcgcgacc
      901 ggcgcgtgcg ccttggggat cgtgaggttc aggaccatct cgatgtcggg atgagcgagc
      961 agctgctcga cgctgaggac gttggccacg ccgaacttct ccgccgcggc ggctgcccgg
     1021 gcgggatcga gatcggcgca ggcgaacatt tccacgttgg ggaaattctt cgccatgccg
     1081 aggtaagcgc cgctgatcgc gccacaaccg acgacaccga ctcgcactgg tttggacatg
     1141 gcggggagaa tactgaagtt caacgggcga aactaggagt agcacgggcg agagaaccac
     1201 aggctcgtag cctgtgctac ggttgatgca tcttcgcgcc gcactggcac gtgaacggct
     1261 tcaattgatg cggacgcaat cgtgattgcg tgctcagttc aatcgagcac ccgcatcgcg
     1321 ggcaggcgat ccgatccccc accttcggcg ccaccggact cggttcatcc agcggcgatg
     1381 gcttggcctc cgccgccccg ccggcgccgg ggatttccgt cacatccgcc cggcggtcca
     1441 tcgctaaatt cgccagctgg tccgccagct cgttcttgtg tcgcaggttg tgctcgatct
     1501 tcgactggtc gaactcgcgg atcagctttt gcgcttcatc gtaaagcgct ttcatgtcgg
     1561 ggttcttcac gcggtattcg ccgcgcattt gcttgatgat caattcgctg tcgccgcgga
     1621 tgaggatttt ttttgcgccc agcttgcggg cttcctccat cgcggtgatc agcgcgcgat
     1681 attcggcgac gttgttcgtc gcccggccga tgaatcgccc gagcgtgatg agcggtgtgc
     1741 catcggcagc cgcgacgacg acaccgatgc cggcaggccc ggggttgccc cggctgccac
     1801 cgtcgaactg tagtgtgagc gtttcatcca ttcggtgtcc ccccgcccgg tcctggtccc
     1861 gcgctgtcga tcggtccatc cgtcgtcgcc gccgcttgtt ttcgaacgtc ttccaatcga
     1921 acgaccaccg tgcccgcggc gatgtcggcg ctgcgctgcc gcagcggcga aacgagcacc
     1981 aggaacaacg ggaacagcag cacgtccagc gcgcgaagca cgttgcggat caagatctgc
     2041 ccacgccccg cggggctgcc atcgacggat accacggtca gaccgaacag ccattttccc
     2101 acggtccggc cggtcagtac ttcgaccagc gtcgtgtgca atatatagac ggcgatggcc
     2161 accccgagcg tgatcagcgc gggcagatct tccagccgct cgcgcggatc gcgcgaaggg
     2221 tccgtgtgta cggacacgac gatcacgcca gcgatcagcg gcagcagatc gaccagccct
     2281 gccccggcgc gcagcgcaag cggcgccggc gggggtgggt tcagttcgcc gccacgcttt
     2341 cgaagcagcg tccggcgata cagcgtcgcc atgactgaaa acgcgagcag cgcgatgagg
     2401 acgccgttga tccaatattc gacggcgctg ccggagaccg tggccggcac ggccaattcc
     2461 gcggcggcgc cgacggcctt gccgtccagg tcgtagcgct gctcgtaaag cttgtcgcca
     2521 tgcagcgcga tgagccgaag gtaaccgcca ccggcggcga tcgcgggcgt gctgtcgggg
     2581 gcgttctcgc cgacccactt catcgggatc gcgcgctgct cgctggcgtc gagataaatc
     2641 cgcccggcgc ccttgccgct gccgacccac agtaccgggt gattttccgt gtcgagcagg
     2701 tcgaagctga cgaacgtctc atccgtctcc ggcgtgatca caccaatgcc gtgccaacgg
     2761 ccgtcggcct gcagcatgag cgtgcggatg cgaccgtcgg tgaggccgac cgcgacgtat
     2821 ggccggccgg ccacgatcgc gagcgagata tcggatgtgg gaccaaccag cggcagcgac
     2881 gcatcgggcg gcagctcggc caccggcgcc caatcgccag attgttgttg gaacagcacc
     2941 agcgaatcgg cgtcggccaa cgcgccgggt tcggcggcgg cggcgggaag agtggtcggc
     3001 tgcgtggccg ccgcacgcgt ggccgcgggc atgccgcctt ttaccagacc gaccgcccag
     3061 agcgtttcgc cgtcgtcgcc gagcgcgcgg atctgcgcgt tggccggcag cggttgaccg
     3121 gtggcagcgc catccggcgc ccagatcgat tgccagctgc cgttgctgag cagcaccacg
     3181 agctgagacg atcgactggc cagcgcggtc acgcgcgtcg gcacgaccgc cagagttttc
     3241 cactgctgcc cgggtccact ggggcggacg aggatgatcg tctgctcgac cggcccgcgc
     3301 ggggtgttct cgacgacgac ccgcgcgacc cagaggcggt cattggaggc tcgcgccagc
     3361 acatcgcgca ccgccgccga cgctggctgg gccagcatca ggagcagagc ggagacgacc
     3421 aacgcgaaac gcttcatcgg aaagaccacg accgacgcga gaatgtcggg gcaggcgggt
     3481 gatttgtaaa gggactggcg cgcggcgctg aacacgccgg gttcggaggt actccgacgg
     3541 cccggattct tcaggcgcgc aagaagcatc cgggtcgtcg gagtacctcc gaacccggcg
     3601 tgggaacttg agaagcggat tggacaacct ttatcctggc acgggcctcc ggcccgtgct
     3661 tagtgctggg agcagcagag cacgagtttc tgaatttaat gcatcatgca cgggccggag
     3721 tcccatgtca cggtgaggct ctagaagttc tgaaccagtt cttagtgcac ttgcgcggcg
     3781 gacgtgatgt tatccgcgca atcgcggtcg atttgaatta cttccttcgt ccccggttcc
     3841 tcgggccgat ggaacgccgc gggacgtggg gaaccattgt gctccgtcgc ggcgttcgtg
     3901 aaatcgaagg tcatacgcga gccttcgtcc ggaaaataca gctcgtaatg ccgggccacc
     3961 tgcggccact ggtcgcgcgg catgcccggg acttccagcg gaccgtggtt gcgcagatac
     4021 gcgcgcagga tcacccggcc gttttcgtcg aacaggtaca cgcgccgcgg ctgcttagtg
     4081 gcgcggtcga accagatttc cttttgtgcc gtccatcgat ccgaccgctt gatgttccac
     4141 acgaacatgt agcagtcggc atcgttgttg aatcgcatca ccggcaccgg cgattcaaga
     4201 aagtccacgt cgaacagccc cacccccagc accgccagca ccaggtccgg tcgcacgggg
     4261 atctttcggc aacacggttt ttcgatgttc gcgtaatgcc cccaccatgt ggtgtcggcg
     4321 ccggcggtga cgatcatcca gaattcggtg tcgttgctgc cgatcgtgaa gatcgtgccg
     4381 gccacgtcct tctgcccgcg gagcagcagc gacatcggcc ggcgatacag caggctgccg
     4441 tcgccggaga cgacgttgga tgtgccgcgt tccttgtcca cgatcgtcgc gttgtattcc
     4501 agctgcgccc agatcgatgg gacttttatg ttgttcgcgt tgatcgcctg gacgacctcg
     4561 accatcggct gggtcgggcc ggtataaacc ttcgccggcg acggttccgg ccggcgacac
     4621 gcggggagca gtgctgctgc aaacagcagc aaacgatgaa cgatgaacga cgaacgatgg
     4681 gtgacatgca tttcgcgttc aacgttcatc gttcatcgct cctggttcat tccttcacag
     4741 ctcttcgttc aagatctgcc ccagttgctg gctcacgtcc gtcacctgct gctcgtcgag
     4801 gcgcgggttc accaggtcca tccgctcttc tttgccgcgg aagcgcatcg tccagatgtc
     4861 ctggccgttt tcttcgcgac tggactcgta cacgagcatc cgcttgcgca tcaggatcaa
     4921 gccgagcacg aagcggaagt tcaacttcgc cggctcggcg gtgtccttca atcgctcaaa
     4981 cagttcgcac agcacttcgt catcgacgaa cagttttttc ttcgcctcat gctgcggaat
     5041 gacggtctgc cagaacgcga gcaggtcttc gcggttgaac gccagccagc aatcaagcga
     5101 cacgtcgagc cgctcgagac cctgcggcgt ttcgcgcagc gctgccatga tccgcgtgcc
     5161 cgcgggcagc tcggcgttgc acacgcagca ccggccgctg ggccggccga cttcgtactg
     5221 accgggtttg gcggcgggtt tgttttccgc gggatgggcg gtggcggcag cggtggtgga
     5281 actcatgtgg cgaaatcctc cgtgaacaca cgacatgata gtcgaggatg ggcaagacgc
     5341 cagacgagcg gtttttgtac cggagtatcc gagcgcgcaa tagtagccgc aggcgatagc
     5401 cagcggggtc ttccgaagct cggcggggct ggcgctcgaa agccccgctg gctgtcgcct
     5461 gcggctatca aagatcttct atcagtcggt gcaacttcag cgcccgcgtc ggcggcccag
     5521 tagcaggatg ccggtgatcg ccaaaccaat cgcgctcggt tccgggacgg cgtaggtgat
     5581 ctgcagcgat gggcgatccg ccagcgtgcc gaattcggat gagtcgaacc gccagccgtc
     5641 ggtgctgttg gccagcagcg accagccgaa gttggtcgcg ccgttcgtcc acgcctgcac
     5701 ggtgtcggtg acgtcgaaca acgcggcgtt gtccagctcg ttggggatca acgtgaagtc
     5761 cgcggacgat gacgcatcaa cgttattcgc gtcgacaccg ttgccgccga agctgccgcc
     5821 ccaggtgctg ttctcgtccc aactgctcaa catgcggtgc agttcgacgg agccgccggt
     5881 gttgtcgttg gtggtcgaac cggtgtggat gttcagcttt gccgacagga tcttcgcgcc
     5941 cgccggcact tggccggcgc cggagccgat gatgttgtcg aaccgcacca gcgcctgcac
     6001 cggctggttg ccggtggcgg tgttgtcgtc ctgatcgacg accagcggcg tcgtgctgcc
     6061 gaacgtcgag ctggtcgagt cctggcgaat ttcggtgtcg tgcgcggcgg cgtagccgtt
     6121 gacgccctgc tggaagctcg cgatcgattg agccgcatcg gtggtgaaga cgccagtaat
     6181 atctttgacg ttcaccgcgc taccggacgg ggcggcgttg aagttgtcgc cgatcgttcg
     6241 cagcagattg tgcagcgtgt acgttgtgtt gttgatcgtg ccgttcttca gattcgcgcc
     6301 ggaaagaatc gtcgggatgc gattgttgct ggcgctgttg tcctcgtccc acgtgacgac
     6361 cagcaggctg ttgtgcgcct tcgcccaggt gacgtacggg tcgatgttcg tcttcagcca
     6421 accgtcggcc tgcgcgatcg tgccgtcgtg catgtcgttg ttctggtcgg gcacgacgaa
     6481 cgaaacggt
//
LOCUS       DATIMH010000546         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1290298, whole genome shotgun sequence.
ACCESSION   DATIMH010000546 DATIMH010000000
VERSION     DATIMH010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290298"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..901
                     /locus_tag="VLI90_09530"
     CDS             128..901
                     /locus_tag="VLI90_09530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV14490.1"
                     /translation="MSNAAVSETAIAPHPELQGFYRGTENKRSFLRHIFDETATDYDR
                     VERLLALGSGRWYRRQALVRSGLVAGMRVLDVATGTGLVAREAIGIVGLAGRVVGVDP
                     SAGMIGQAHASLPGLAAVLGCGEALPLADGQFNFVSMGYALRHLPDLHVAFAEFARVL
                     RPVGRLCVLEITRPHGVIRRQLLRTYFRVALPVAERVIHTSPATRRLWQYYWETIDQC
                     VTPAVVLGALRDAGFARVEQRLSMGLFSEYTAMKPDSVG"
     gene            complement(1023..1541)
                     /locus_tag="VLI90_09535"
     CDS             complement(1023..1541)
                     /locus_tag="VLI90_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSV14491.1"
                     /translation="MNRQSLRITVVSLGLGFMAACSSSYSPLYKAADRNDAAAIQQLT
                     SSGASANAKEHNNRVPLHAAAAKGNLDAAKALLAAGADPNAQDDRGDTPLMTAASHGY
                     LPMVNAMLDKHPPLDTQNKRGATALQFAVEGDKAEVAKALVKAGANPNLTDNQGRSAT
                     SLAAAKGMSLTP"
BASE COUNT          262 a          536 c          644 g          376 t
ORIGIN      
        1 gctgtgaaat ctgtggctaa gctctggctc ttctcggcgt ccgcctcggc gtctcggcga
       61 ttgcatttgt ctgttctatc cagcgcacgc gcagcggtcg aggccaaaga tgggtacatt
      121 cacgcccatg tcaaatgccg cggtcagcga aacggcgatc gcccctcatc cggagttgca
      181 gggcttttac cgcgggacgg aaaacaagcg cagctttttg cggcatatct ttgacgaaac
      241 cgcaacggat tacgaccggg tcgagcggct gctggcgctg ggcagcgggc ggtggtaccg
      301 ccggcaggcg ctggtgcgga gtggtctagt ggcggggatg cgcgtgctgg atgtggccac
      361 gggcacggga ttggtggcgc gggaagccat tgggatcgtc ggattggcag ggcgagtcgt
      421 tggtgttgat cccagcgcgg ggatgatcgg gcaagcgcac gcgtcactgc cgggcctggc
      481 cgcggtgctg ggttgtggtg aagcgttgcc gctggcggat gggcagttta atttcgtctc
      541 gatgggatat gccctgcggc atctgccgga cctgcacgtg gctttcgcgg agttcgcccg
      601 cgtgctgcgc ccggtcgggc ggttatgcgt gctggagatc acccggcccc acggcgtcat
      661 ccgccggcaa ctgctgcgga cctatttccg cgtggcgctg ccggtggcgg agcgggtcat
      721 ccacacatca ccggcgacgc ggcggctgtg gcagtattac tgggagacga tcgaccagtg
      781 cgtgacgccg gcggtcgtgc tgggggcgct gcgcgacgcg gggttcgcgc gcgtggagca
      841 acggttgtcg atgggattgt tttctgagta cacggcgatg aagccggact cagtggggta
      901 aagaaccgcg cccacgccgg gttcggaggt actccgacga cccggatcct tggcgcggaa
      961 gaacccggat cgtcgcgagt acgctacccg gcgtggtgtc acgttgaaag tctcctcagc
     1021 gctcacggcg tcagcgacat gcccttcgcc gcggcgaggc tggtcgcgct gcggccctgg
     1081 ttgtcggtta ggttcgggtt cgccccggct ttcaccagtg ccttggccac ttcggctttg
     1141 tcgccttcaa cggcgaactg caacgctgtc gcgccgcgct tgttctgcgt gtccagcggc
     1201 ggatgcttgt ccagcatcgc gtttaccatc ggcaggtatc cgtgtgacgc ggcagtcatg
     1261 agcggcgtgt cgccgcggtc gtcctgggcg ttgggatcgg caccggcggc gagcagcgcc
     1321 ttggcggcgt ccaggttgcc cttggcggcg gcggcgtgca gcggcacgcg gttgttgtgt
     1381 tccttggcgt tggccgaggc gccgctgctg gtcagctgct gaatggcggc cgcatcgttg
     1441 cggtcggccg ccttgtacag cggggaatac gacgacgagc acgcggccat gaaaccgagg
     1501 ccgagggaaa cgacggtgat ccgaagcgat tgacgattca tgagtgactc ctctttccca
     1561 gaaatggttc tcctcgcaat cgacgcgcgg gatatgttac cctgctgccg gacgtgagca
     1621 atatcgcgtg tggatgttga cagcaacctc agattttttc gcttcaggcg cgcatcgctg
     1681 cggccttgcc tccgttaggt gggccatcgc cttgtcgatc ttgctcgcgt ccccttgcgc
     1741 gcttgccgag caagtcgaga gcatctccac cgggtcgcgc acgttcagtt ttggcgcctc
     1801 atacatcgcc ggcatcga
//
LOCUS       DATIMH010000547         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3307585, whole genome shotgun sequence.
ACCESSION   DATIMH010000547 DATIMH010000000
VERSION     DATIMH010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3307585"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..>1574)
                     /locus_tag="VLI90_09540"
     CDS             complement(189..>1574)
                     /locus_tag="VLI90_09540"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HSV14492.1"
                     /translation="ILPAGLKTVTGSPVIQCRPRQTTPNPTENRAAPMADTLTITDNR
                     TGKQYELPIADGAIRATDLRQIKTAEDDQGLVTYDPAFMNTAACRSRITYIDGDKGVL
                     EYRGYPIEQLAERSTYLETAYLIIFGELPTDAQLKEWTKQITFHTMLHENIKKLMEGF
                     QYDAHPMGVFLSTVGAFSTFYPDAKKIFDEQSRQKQVLRLVAKVPSIAAYAYRHSIGR
                     PYVYPDNDLSFTGNFLNMLFKMTELKYQPSPVLERALDILFILHADHEQNCSTSAMRG
                     IGSSHVDPYSAMAGAAAALYGPLHGGANEAVLRMLTEIGSLQNVPAFIKRVKAGEGRL
                     MGFGHRVYKSYDPRAKIIKKTADEVFEVTGKNPLLDIALELERIALQDDYFVTRKLYP
                     NVDFYSGLIYQAMGFPVTMFPVLFAIPRTSGWIAQWEEMLLDPDQKIARPRQLYIGAA
                     KRDYAPLEKRG"
BASE COUNT          274 a          454 c          512 g          334 t
ORIGIN      
        1 gttctcttgc accaatacac gaagctcctt tgcactgact aggcgaagcg tccgtctgca
       61 ccgacaaggc gaagcgtttg caccgacaag gcgcagcgcc cctctgcacc actaggcgaa
      121 gcaccccgct ttgcgggcga agcgtccgtc tgcactgact aggcgcggtt tccgcgcggt
      181 gtcgggtgct atccgcgctt ttcgagcggg gcgtaatcgc gcttcgcggc gccgatatag
      241 agctgccgcg gtcgcgcgat cttctgatcc gggtcgagca gcatttcctc ccactgtgcg
      301 atccagccag aggtgcgcgg aatcgcgaac agcacgggga acatcgtcac cgggaaaccc
      361 atggcctgat aaatgagccc tgagtagaag tcgacgttcg gatagagctt gcgggtgacg
      421 aagtagtcgt cctgcagcgc gatgcgttcg agctcgaggg cgatgtcgag cagcggattc
      481 ttgccggtga cctcgaacac ttcatcagcg gtcttcttga tgatcttggc gcgcgggtcg
      541 tacgacttgt agacgcgatg gccgaatccc atcagccgtc cttcgcctgc cttgacgcgc
      601 ttgatgaacg ctggaacgtt ctgcaggctg ccgatctccg tcagcattcg cagcacggct
      661 tcgttggcgc caccgtgcag cggcccgtac agcgcagccg cagcgccggc catcgccgaa
      721 tacggatcga cgtgcgagct gccgatgccg cgcatcgcgc tcgtgctgca gttctgctcg
      781 tgatcggcgt ggaggatgaa gaggatgtcg agggcgcgtt cgaggacggg gctgggctga
      841 tatttgagct cggtcatctt gaacagcatg ttcaggaagt tgccggtgaa gctcaggtcg
      901 ttatctggat aaacgtacgg tcggccaatg ctgtggcgat aggcgtaggc cgcaatcgac
      961 ggcaccttgg cgaccagacg cagcacctgc ttctgtcgcg actgctcgtc gaagattttt
     1021 ttggcgtcgg ggtagaacgt ggaaaaggcg ccgaccgtgc tgaggaaaac gcccatcggg
     1081 tgggcatcgt actggaatcc ctccatgagc ttcttgatgt tctcgtgcag catcgtgtgg
     1141 aaggtgatct gtttcgtcca ctccttcagc tgcgcgtcgg tcggcaactc gccaaagatg
     1201 atgagatacg ccgtttcgag atacgtcgag cgttcggcca gctgctcgat cgggtacccg
     1261 cggtattcga gcacgccctt gtcgccgtcg atgtaggtga tccggctgcg gcaggcggcc
     1321 gtgttcatga acgccggatc gtaggtgacc agcccctgat cgtcctcagc cgtcttgatt
     1381 tggcgaaggt ctgtggcgcg aatcgcgccg tcggcgatgg gcaactcgta ttgcttgccg
     1441 gtgcggttat cagtgatcgt cagggtgtcg gccataggtg cagcgcggtt ctcagtcggg
     1501 ttcggcgtcg tttgtcgcgg acggcactgg atcaccggcg acccggtaac ggtcttcagc
     1561 ccagcgggca agat
//
LOCUS       DATIMH010000548         4179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4462850, whole genome shotgun sequence.
ACCESSION   DATIMH010000548 DATIMH010000000
VERSION     DATIMH010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4462850"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2730)
                     /locus_tag="VLI90_09545"
     CDS             complement(<1..2730)
                     /locus_tag="VLI90_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEPR-XLL domain-containing protein"
                     /protein_id="HSV14493.1"
                     /translation="MSNRIHSATVRRSVRHSAHELRRSIEALEPRVLMAANAWKAAVA
                     GDWSDPTKWSAGHVPTSSEDVSIAVAGSYIVTHNQGTTDSVKSLTISATSATLELIGG
                     KVQVSGATSVSGGTLTLQGGGLLKGTLATSGTGKFTITGSGILDGVTIASDLPVASGT
                     LFLYHTVTLNSGKKITTTDATVYLYDQPDGGASLLGSGEVVLGGVSGGNVYLYNGSGH
                     NATIASTIKVHGFGTVYFYGGTNNSTIQADQNGKTLSVTGATWINKGTLSATNGGILT
                     VSGTWSSPSPGAINENASTLNLAGAFDTIPLPTRTAGAINVTGTYDLKGSTLALTATT
                     GPWNLQGGTIKNGTISQTSATLLNVVGSGTLDTMTIASDFTFSSATLFLYHANTLSTG
                     KKLTDNDGTLYLYDQPTGGASLNGTGEVILAGASGGSVYFYNGSGHLATISTTIKIHG
                     YGTVYFYAGTNNATITADVNSKTLTVTGATWSNKGTLSAINGGNMTVSGTWSSPSPGV
                     LSENASTLTLSGTFSNIVLPTRTAGTILLNGVYDLANGTLALTPTTGSWNDQGGTVKN
                     ATLNFTGGATLSIVGSATFDHVTFAKDYTVTSGTMFLNHANTLATGKKIIVNDGTLYL
                     YDTATGGASLGGTGEVVLGGASGGTVYFYNGSGHLATLASTILTHGYGTVYFYTGESN
                     TGTIQADVSGKTLSASGSTWVNKGTLSAINGATLTVSGTWSSPSPGKTTETNSTINLS
                     GTFTNITLPVRTGGTILMNGIYDLAGSTLALTATTGSWNFQGGTIKNGSLSLAGGANL
                     AIIGSATLDKVTLASNYTVASTTLFLIHACSLASGVQLTANLGTIYLYDQADGGASLN
                     GSGEVILAGATGSSFYLYNGSGHLASIGSGVRVHGFGTFYSYGSVNNGIVEADV"
     gene            3155..>4179
                     /locus_tag="VLI90_09550"
     CDS             3155..>4179
                     /locus_tag="VLI90_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSV14494.1"
                     /translation="MKRQLAVFCLTATAVGLVLLPGCRSNSQPAPPPAVESTGNNTPP
                     PTTTVAQPPTTNTATLSGSDIVATVNGWNITRQELEQPLIDAHGLTILLNLVQLNLAK
                     QQTTKMGLHVSPEDVQRERQVTFNKLFPDATAGDDENLLDQFLRQQHSSRAEFDLTIE
                     TNAHLRKAAEVQLPGKITEEMMHSAFGQLYGENRQVRDIELGNMREVGAAQARLAKGE
                     PFAQVAQEMSLDRRTAAVGGELPPFSAQSPNIPQAIKDIAQTLKQGQVSPDAVVVESK
                     YHLIKLERIIPPKVVKYEDVKDSVRKTLEEQWIEFAMREYRNQLAQVAVQTMNIRDPV
                     LAEQWKR"
BASE COUNT          745 a         1292 c         1351 g          791 t
ORIGIN      
        1 gacgtctgct tcgacgatgc cgttgttgac cgagccgtac gaatagaacg tcccgaagcc
       61 gtgcacgcga acgccggagc cgatggacgc caggtgcccc gagccattgt aaagatagaa
      121 actgctaccg gtcgctccag cgaggatgac ttcaccgctg ccgttcagcg acgcgccacc
      181 gtcggcctgg tcgtacaggt agatcgtgcc gaggttggcg gtcagttgca caccgctcgc
      241 caggctgcag gcgtggatca agaacagcgt ggtgctggcg accgtgtagt tggacgcgag
      301 ggtgaccttg tccaacgtcg cgctgccgat gatggctaag ttcgcgccgc cggcgaggct
      361 caacgatccg ttcttgatcg tcccgccctg gaagttccac gatcctgtcg tggcggtcag
      421 cgccagcgtg ctgccggcca gatcatagat gccgttcatc aaaatagtcc cgcccgtgcg
      481 caccggcagg gtgatgttgg tgaacgtgcc ggacaggttg atggtggaat tggtctcggt
      541 ggtcttgccg gggctggggc tggaccaggt gccgctgaca gtaagagtgg cgccgttgat
      601 ggctgagagc gtgcccttgt tcacccacgt cgagccgctg gctgatagtg tcttgccgct
      661 cacgtcggcc tgaatcgtgc cggtgttcga ctcgccggtg tagaaataga ccgtgccgta
      721 gccgtgcgtg aggatggtgc tggccagcgt cgccaggtgt ccggagccgt tgtagaagta
      781 aacggtgccg ccgctcgctc cgcccagcac gacttcaccg gtgccgccca acgaagcccc
      841 gccggtcgcg gtgtcgtaca ggtaaagcgt gccgtcgttc acgatgatct tcttgcccgt
      901 cgccagcgtg ttggcgtggt tcaggaacat cgtgccggag gtgacggtgt agtctttggc
      961 gaaggtcacg tgatcgaagg tggcgctgcc gacgatgctc agcgttgccc cgccggtgaa
     1021 gttcagcgtc gcgttcttga ccgttccacc ctggtcgttc cacgaacctg tcgtcggcgt
     1081 cagcgccagt gtgccgttgg ccaggtcgta gacgccgttg agcagaatcg tcccggcggt
     1141 gcgcgtcggc aggacgatgt tgctgaatgt gccgctcagc gtcagcgtcg aagcattttc
     1201 actcagcacg ccggggctgg ggctggacca cgtgcccgag acggtcatgt tcccgccgtt
     1261 gatcgccgac agcgtacctt tgttgctcca cgtcgcgccg gtgacggtca gggtcttgct
     1321 gttcacgtcc gcggtgatcg tcgcgttgtt ggtgccggcg tagaaataga cggtgccgta
     1381 gccgtggatc ttgatcgtgg tggagatcgt cgccagatga ccggaaccgt tgtagaagta
     1441 aacgctgccg ccgctggctc cggcgaggat cacttccccc gtgccgttca acgatgcgcc
     1501 gccggtgggc tggtcgtaca ggtaaagggt gccgtcgttg tcggtcagct ttttgccggt
     1561 ggataacgtg ttggcgtgat agaggaacag cgtcgcgctg ctgaacgtga aatcgctggc
     1621 aatcgtcatg gtgtccaacg tgccgctgcc gacgacgttc agcagcgtgg cgctggtctg
     1681 gctgatcgtg ccgttcttga tcgtgccgcc ctggaggttc cacggtccgg tcgtcgccgt
     1741 cagcgccagc gtcgagcctt tcaaatcgta ggtcccggtg acgttgatgg caccagcggt
     1801 gcgcgtcggc agggggatgg tatcgaacgc tccggcaagg ttcagcgtcg atgcgttttc
     1861 gttgatggcg cccggactgg ggctcgacca cgtcccgctg acggtgagaa tgccgccgtt
     1921 ggtcgccgac agcgtcccct tgttgatcca ggtggcgccg gtgacgctca gcgtcttgcc
     1981 gttctggtca gcttgaatgg tcgagttgtt ggtgccgcca taaaagtaga cggtgccgaa
     2041 accgtgcacc ttgatggtag aggcgatcgt cgcattgtga ccgctgccgt tgtacaggta
     2101 gacgttcccg ccactcaccc cgcccaggac cacctcaccg ctgcccaaca gcgacgcgcc
     2161 gccgtccggt tggtcgtaca ggtagacggt ggcatcggtc gtggtgatct tcttcccgct
     2221 gttgagcgtt acggtgtgat agaggaacag cgtgcccgag gcgacgggca gatcgctggc
     2281 gatcgtcacg ccgtccagga tgccgctgcc ggtgatggtg aacttgccgg tgccgctggt
     2341 ggccagcgtg cccttaagca gcccgccgcc ctgcaaggtc agggtgccgc cgctgacgct
     2401 cgtcgcgccg ctgacctgca ccttcccgcc gatcagttcc agcgtcgccg acgtggccga
     2461 gatcgtcagg ctcttcaccg agtccgtcgt gccctggttg tgggtgacga tgtaactgcc
     2521 ggccaccgcg atcgacacat cttcgctgga cgttggcacg tgtccggccg accatttggt
     2581 cgggtcgctc caatcgccgg cgacggcggc cttccacgcg ttggcggcca tcagcacgcg
     2641 cggctccagc gcctcgatgc tccggcgaag ctcatgcgct gaatgacgaa cggaacgacg
     2701 aacggttgcc gaatgaatgc ggttggacat gtctcttcct ccaggagaag cagcgcgaag
     2761 cacgcgcgca tgcgtgcaca gcgcaatcga tcgatctgtg aaacggcgaa cgccttttcg
     2821 ttatgtgggg aaaaacaccg cgcacccagc aggccgataa ccgagtccgc gcctgacaac
     2881 atatcaatac agaatcagcc gatgcgcacg cgcggacggt taaggcgggg gatttgtgtc
     2941 ggtcgtacat gctgattggg tgcccgtgtg aactggatac acccaatcgg caccaattct
     3001 gcgccggtcc cgccgcttgc ggcttcgcaa cccaggaggc gcggtttacg aagccgcagc
     3061 ggcaacagag acatccaacc ctgcccatct tgacacttca cccccacctt ctaatacttc
     3121 cgcgttcgtc gtcgttcgcc cgaggagtct tcctttgaag cgacaattgg ccgtgttttg
     3181 cctgaccgcc accgcggtgg ggctggtcct gctgccgggt tgccgcagca acagccaacc
     3241 tgcgcctccg cccgcggtgg aatcgaccgg caacaacacg ccgccgccga cgacgacggt
     3301 cgcccaacca ccgacgacca acaccgccac cctttccggc agcgacatcg tcgccaccgt
     3361 caatggctgg aacatcaccc gccaggaact cgaacagccg ctcatcgacg cccatggtct
     3421 gacgatcctg ctgaacctcg ttcaactcaa cctcgccaag cagcagacga ccaagatggg
     3481 cctgcacgtg tcgcccgaag acgtgcagcg cgaacggcag gtcacgttca ataaattatt
     3541 tcccgatgcg acggccggcg atgacgagaa cctgctcgac cagttccttc gccagcagca
     3601 cagcagccgg gcggagttcg atctgacaat cgaaaccaac gcgcatcttc gcaaggccgc
     3661 cgaggtgcag ctgccgggaa aaatcaccga agagatgatg cacagcgcgt tcggccagct
     3721 gtacggcgag aaccgtcagg tgcgcgacat cgagctgggc aacatgcgcg aggtcggtgc
     3781 ggcgcaggcg cggctggcca agggcgaacc atttgcacag gtcgcgcagg agatgagcct
     3841 ggaccgccgc accgcggccg tcggtggtga actgccgccg ttcagcgcgc agtcgccgaa
     3901 catcccgcag gcgatcaagg acatcgcgca gacgttgaag caagggcagg tctcgcccga
     3961 cgccgtcgtg gtcgagtcga aatatcactt gatcaagctc gagcgcatca tcccgccgaa
     4021 ggttgtgaag tacgaagacg tgaaggacag cgtccgcaag acgctcgagg agcagtggat
     4081 cgaattcgcg atgcgcgaat accgcaacca gctggcgcag gtggcggtgc agacgatgaa
     4141 catccgtgac cccgtgctgg ctgagcagtg gaagcggca
//
LOCUS       DATIMH010000549         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2152681, whole genome shotgun sequence.
ACCESSION   DATIMH010000549 DATIMH010000000
VERSION     DATIMH010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152681"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..769)
                     /locus_tag="VLI90_09555"
     CDS             complement(113..769)
                     /locus_tag="VLI90_09555"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HSV14495.1"
                     /translation="MDQPRPDSIPNPAVRRLSLYLRQLEAFKRKDRRTISSKQLGESL
                     NLTDAQVRKDLAYFGQFGHPGIGYRVDDLIGQVKRILGTDKTWNVLLIGAGNLGRALM
                     SYRGFDAKGFKLVAVFDSAPEKAGTHEGDFLIQPLSEMVPTIQRLNIRLGILAVPADV
                     AQDVADQLVAAGVRGLLNFAPVSIHVPSDVALNAVDLAVQLEQLSFQVNVSPKLTALS
                     "
     gene            929..2299
                     /locus_tag="VLI90_09560"
     CDS             929..2299
                     /locus_tag="VLI90_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HSV14496.1"
                     /translation="MRAKQFIGRGFPVAIETLEHRQMLSAAVSSTALASSINPAIFGQ
                     SITLTATVTSKSGSPGGTVTFKDGSTTLGTAAVNTTTHHATFSTAKLSVAAHNLTAVY
                     GGTASFAKSSSAIIKETISADATKTTLTASTLAPVLGQSVTLTATVTAAAPGSGVPKG
                     TVQFKQGSTVLGSASLDSTGHAAFKIYTLFFGTHAITATYTGGTSFKASTSTAATVVV
                     KQAALTTTADGLKLATVAAGSGPGVVVQQDLKVDYTGYLTNGVKFDSSLNAGRTPFDF
                     ELGVHQVIAGWDEGLFGAKVGETRVLVIPPALGYGSQANGSIPANSTLIFIVKMLAFD
                     TTPKITLTGKAAIANNAAASTASGTNFGSIKVGSSSATLTLTIGNTGNAPLQPTANPP
                     VIISGTNPGDFVITQPIQSSPGGPIQFTIVFKPKATGTRTAKITIRTNDPLTPNFTFT
                     VSGIGT"
     gene            2555..3436
                     /gene="argB"
                     /locus_tag="VLI90_09565"
     CDS             2555..3436
                     /gene="argB"
                     /locus_tag="VLI90_09565"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HSV14497.1"
                     /translation="MHMDAAINKASGLVEALSYIQRFHNKVVVVKVGGSIMDDEQALT
                     NLLTDIVFMNYVGMQPILIHGGGKAINEAMAKAGLEPQMVQGRRYTDERTLAIAEHVL
                     CNQINKFIVNFIQAQGCEAMGLHALTSNVLFAEKTFLQGPDGRRIDLGFVGEVKSVNA
                     RLLQLLVQADSIPCIATIARDAAGQRLNVNADTAAGAVAAAMKAEKFVVVSDTHGIRS
                     DPKDPESLVKHLTVGQIDEMVKSGAIGSGMLPKVEACVQAIRAGVTKTHIIDGRIPHA
                     LLLEIYTEAGIGTEIVL"
BASE COUNT          696 a         1100 c         1083 g          611 t
ORIGIN      
        1 gatcacgcgg cgcggtttgc gaaaccgcaa gcgactgcag cgccaatcga gcaactacgc
       61 gttcacccac tgccggctgt atccttcgac tccggcggtg taccgcctgc gctcaggata
      121 aagcggtcag cttcggcgac acattcacct gaaacgacag ctgctccaac tgcaccgcta
      181 aatccaccgc gttcagcgcc acatccgacg gcacgtgaat cgacaccggc gcgaagttca
      241 acagccctcg caccccggcc gccaccagtt gatcggccac atcctgcgcc acatccgccg
      301 gcaccgcgag gatgcccagg cggatattta atcgctgaat cgtcggcacc atctcgctca
      361 gcggctgaat taaaaagtcg ccctcgtgcg tgccagcttt ttccggcgca gaatcaaaca
      421 cggcaaccag tttgaaacct ttggcgtcaa acccgcgata cgacatcagc gcgcggccca
      481 gattgcccgc gccgatcagc aacacgttcc acgtcttgtc ggtgccgagg atgcgcttga
      541 cctgcccaat cagatcatcg acgcgataac cgatgccggg gtgcccgaac tggccgaagt
      601 atgccagatc ttttcgcacc tgggcgtcgg tcaggttcag gctttcgccg agttgtttgc
      661 tggagatcgt tcttcgatct ttgcgtttga aggcttccag ttgccgcagg taaaggctga
      721 gccgccggac ggcggggttg gggatggaat cgggtcgcgg ttgatccatg caggtcctcg
      781 gcgctccgac acgcgcgcgt cggcgatgta agggattatt tcaggccgcc gaacgccgtc
      841 aagcggacga tcgtagcgtg aagctgcgac gcatgcgatc gccgactgaa tttggtatag
      901 tgctgctttc gttttggagg ctgtgagaat gcgggcaaag cagttcatcg ggcgcggatt
      961 ccctgtcgct attgaaaccc tggaacaccg gcagatgctg tcggcggcgg tgagtagcac
     1021 cgcgctggca tcgtcgatca acccggcgat cttcggccag agcatcacgc tcaccgccac
     1081 cgtcacgagc aagtccggca gccccggcgg caccgtcacg tttaaagacg gcagcaccac
     1141 cttgggcacg gctgccgtca acacgacgac gcatcatgcc acattttcga ctgcgaaact
     1201 ttcggtcgcc gcacacaact tgacggccgt ctacggcggg accgcatcct tcgcgaagag
     1261 cagttcggcg atcatcaaag agacgatcag cgcggatgcg acgaagacga cgctgacggc
     1321 gtcaacgctg gcgccggtgc tcggccaatc ggtgacgctg accgccaccg tcaccgccgc
     1381 cgcgccgggc agtggtgtgc ccaagggcac ggtgcagttc aagcaaggtt cgactgtgct
     1441 cggcagcgca tcgctcgatt cgaccggtca cgccgccttc aagatctaca cgctgttctt
     1501 cggcacccat gcgatcaccg cgacgtacac cggcggcacg tcgttcaaag ccagcacgtc
     1561 aaccgcggct acggtcgtcg tgaagcaggc agcgctgacg acgacggcgg acgggctgaa
     1621 gctggcgacc gtcgccgccg gcagcgggcc gggagttgtg gttcaacagg atctgaaggt
     1681 cgattacacc ggctatctca ccaacggcgt aaagttcgac agctcgctaa acgcggggcg
     1741 aacgccgttt gatttcgagc tgggcgtgca ccaggtgatc gccgggtggg acgaaggttt
     1801 gttcggcgcg aaggtcggcg agacgcgcgt gctggtgatc ccgccggcgc tgggttacgg
     1861 ctcgcaagcc aacggctcga tccccgccaa ctccacgctc atcttcatcg tcaaaatgct
     1921 ggcgttcgac acgacgccga agatcacgct gaccggtaag gccgcgatcg ccaacaacgc
     1981 cgccgcgtcg acggcgagcg gcacgaactt cggcagcatc aaggtcggct cgtcgagcgc
     2041 gacgctgacg ctgacgatcg gcaacaccgg caacgccccg ctgcaaccga cggcgaatcc
     2101 gccggtgatc atcagcggca cgaaccccgg cgatttcgtc attactcaac cgatccaatc
     2161 gtcgccgggc gggccgatcc aattcacgat cgtgttcaag cccaaggcaa ccggcacgcg
     2221 cacggcgaag atcacgatcc gcaccaacga cccgctgacg ccgaacttca cgttcacggt
     2281 ttctggaatt gggacttaga aaatcggtga tctctcctgc tcctgcatcg ctcgcacggg
     2341 ccggaggcct gtgtcacgac gaaggtatca aatgctgaac gcgacacgcg gcctccggcc
     2401 cgtgcgagcg gcgcgcgaag tcaactcgct ttccccttcg cgtctgtctt cgccccgtcg
     2461 cgtcttcgcg ctcgcattcg attcacaaac gtttttgaca caccgcccgg gcattcctaa
     2521 actcaccgtt tctttttcgg agccgacttc agcaatgcac atggacgcgg cgatcaacaa
     2581 agcgagcggg ttggtcgaag ccctctccta catccagcga tttcacaaca aggtggtcgt
     2641 cgtcaaggtc ggcgggtcga tcatggacga tgagcaggcg ctgacgaacc tgctcaccga
     2701 catcgtcttc atgaactacg tcggcatgca gccgatcctg atccacggcg ggggcaaggc
     2761 gatcaacgaa gcgatggcca aggcggggct cgaaccgcag atggtgcagg gccggcggta
     2821 taccgacgag cgcacgctgg cgattgccga gcacgtgctg tgcaaccaga tcaacaagtt
     2881 catcgtgaac ttcatccagg cgcagggctg cgaggcgatg ggcctgcacg cgctgaccag
     2941 caacgtgctg ttcgcggaga agacgttttt gcaagggccg gacggccggc ggatcgatct
     3001 gggtttcgtc ggcgaagtga agtcggtgaa cgcgcgattg ctgcagctgc tggtgcaggc
     3061 ggattcgatt ccgtgcatcg ccacgatcgc gcgcgacgcc gcgggacagc ggttgaacgt
     3121 gaacgccgac accgccgccg gcgcggtggc ggcggcgatg aaggcggaaa aattcgtcgt
     3181 cgtctccgac acccacggca tccgcagcga tccgaaagat cccgaaagcc tggtgaagca
     3241 cctgacggtc gggcagatcg acgagatggt gaagagcggc gcgatcggca gcggcatgct
     3301 gccgaaggtc gaggcgtgcg tgcaggcgat ccgcgcgggc gtgacgaaga cgcacatcat
     3361 cgacgggcgg attccgcacg cgctgctgct ggagatttac accgaggcgg ggattgggac
     3421 ggagattgtt ttgtagcacg ggcgtctcgc ccgtggttcg agcgcagcgt cttggatgca
     3481 ctgatggaaa
//
LOCUS       DATIMH010000550         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2152915, whole genome shotgun sequence.
ACCESSION   DATIMH010000550 DATIMH010000000
VERSION     DATIMH010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152915"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VLI90_09570"
     CDS             complement(<1..961)
                     /locus_tag="VLI90_09570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HSV14498.1"
                     /translation="MDATSLSNLNDQQRAAATHGDGPLLIIAGAGTGKTNTLAHRVAH
                     LIAIGVNPARILLLTFTRRAASEMTKRVESIVARSATQDFGELSRAASQLQRLWGGTF
                     HSIANRLLRLHSQSIGLGEAFTVVDRADAEDLLNVVRSELSLDKGDVRFPKKGTCLSI
                     YSRCVNAQEPIEEALKTHFPWCSAYPEQLKQLFKAYVARKQQQNVLDYDDLLLYWFHL
                     MQDDAVAADVRKRFDAVLVDEYQDTNALQAQVLQRMRPDGKGLTVVGDDAQSIYSFRA
                     ATVHNILDFPRVFPNTTVLKLEQNYRSVQPILEATNQVIALCPE"
     gene            complement(1261..>3187)
                     /locus_tag="VLI90_09575"
     CDS             complement(1261..>3187)
                     /locus_tag="VLI90_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14499.1"
                     /translation="RYRGEPASPYVATQEFMMQEAERHGLDYTHLALYLCGDNLYPTI
                     RDVPPVRGEESICPDGRPMGTYKYWSPRAQAAMVEHVRGLLNLYGKGMRRRTVGSSEA
                     IVMQTMFEPIPKPSFDDEGRQRYLAWLQRRYGGDIAKLNQRYQLNPKSFEQLRPDQYW
                     LRPEELNWVGCARPSAADFANRTPDFHRWIDNQTYLADELDEYLRTMKQHWRELETPL
                     FIEPVLHQWGYFFNPPGHTGWQTGQRALDVYRCAEHVDGVLYIASPLNAENRADAMAL
                     SVEASIMRSANRARPFTGGLYLGRHVSGDIYSVVPPAEAIATLIANGATGLHIYGYSG
                     LDDGGVMFRMDQPFKDSLRAGLHWASEVMPLIADQPRTKQVALLFPAEMSLFEPLEVD
                     TEGRHRMDLLGWYAQFVDLGWQVDILHPTQVAGGALAGYTHLVVPHNSLYDLGDNAGL
                     EAAVAQFVIAGGKVFHGPHCELACRALGIEEEPIDFDCIKWREEIIPHGWSTVAYRGG
                     VAIGAYIQSGKTAIAQTSLGAGRVFSFGFEYGYAYSRRTMPIVPPNYGRREIHPIVLL
                     EQTPVAALAGAAPQLPMKPIKAVEFARFGNRLIIVNHRASPIDISVISARRTIPQVPG
                     APGWLAAHSAVVLETDG"
BASE COUNT          563 a         1026 c         1001 g          597 t
ORIGIN      
        1 gctccgggca cagcgcgata acctggttcg tcgcttccag gatcggctga acgctgcggt
       61 aattctgctc cagcttcaac acggtggtgt tcggaaagac gcgcgggaag tcgaggatgt
      121 tgtgcaccgt ggcggcgcgg aaggagtaga tgctctgcgc atcatcgccg acgacggtaa
      181 gtcctttgcc gtcgggacgc atccgctgca gcacctgcgc ctgcagcgcg ttggtgtcct
      241 ggtactcatc caccagcacc gcatcaaagc gctttcgcac gtcggccgcc accgcgtcgt
      301 cctgcatcag gtggaaccag tagagcagca ggtcgtcgta atcgagcacg ttctgctgct
      361 gcttgcgcgc cacgtatgcc ttgaataatt gcttgagctg ctcgggatac gcgctgcacc
      421 acggaaaatg cgtcttgagc gcctcttcga tcggctcctg cgcattcacg catcggctgt
      481 agatcgacag gcaggttccc ttcttcggaa accgcacgtc gcccttgtcc agcgacaact
      541 ccgaccgcac aacattcagc agatcttcgg cgtcggcccg atcgacgacg gtaaacgcct
      601 cgcccagccc gatcgactgg ctatgtaatc tcaacagccg attcgcaatc gaatgaaacg
      661 ttccacccca tagtctctgt agctgcgacg cggctcgact gagctcgccg aagtcctgcg
      721 tcgccgatcg cgcgacgatc gactcgacgc gcttggtcat ctccgatgcc gcgcgccgcg
      781 tgaatgtcag caggaggatg cgagccggat tgacgccgat ggcgatcaga tgggccacgc
      841 gatgcgcgag ggtgttcgtc ttcccggtcc ccgcgccggc gatgatgagc aacgggccgt
      901 ccccatgcgt ggcggcggcg cgctgctggt cgttgagatt ggaaagggac gtggcgtcca
      961 tgcgtgggtt cggtcagtgt tcgggaggct tgtaccgcgc gcgtgcgtga aatcaagcgt
     1021 gaaggcgggt gatatactgc ggtcgcgcgg caatcgcatg tggtcctttc cccctctccc
     1081 ggtacgccgt tggtacgccg ggagagggtt ggggtgaggg tgatttggac tggcgataac
     1141 cgctcgacgt tcgaaatcac cctcaccctg gccctctccc ggtgtaccaa gagaggggaa
     1201 caccagcgcg cccaacatac atgcgtttgc ctgggcgcgg tcaaatcacg tcatcgcgat
     1261 tcacccgtct gtttccagaa cgaccgccga gtgagccgcc agccacccgg gggcgccggg
     1321 gacttgcggg atggtacgtc gcgcagaaat cacgctgatg tcgatcgggc tggcgcggtg
     1381 gttgacgatg atcaaccggt tgccgaagcg ggcgaactcg acggctttga tcggcttcat
     1441 cggcaactgc ggcgccgcgc cggccagcgc ggcgacgggc gtttgctcaa gcagcacgat
     1501 cggatgaatt tcgcggcggc cgtagttcgg cgggacgatc ggcatcgtcc gccgcgaata
     1561 ggcgtagcca tattcgaagc caaacgagaa gacgcggccg gcgccgaggc tcgtctgggc
     1621 aatcgcggtc ttacccgatt ggatgtacgc gccgatggcg acgccgcctc ggtaggcgac
     1681 ggtggaccaa ccgtgcggga tgatttcttc gcgccatttg atgcagtcga aatcgatcgg
     1741 ttcctcttcg atgccgagcg cgcggcaggc aagctcgcaa tgcgggccgt ggaacacttt
     1801 tccgcctgcg atgacgaact gcgccaccgc cgcctcgagg cccgcgttgt cgcccaggtc
     1861 gtacagcgaa ttgtgcggca cgacgagatg cgtgtaaccg gcaagcgcgc cgccggcgac
     1921 ctgcgtggga tgcaggatgt cgacctgcca gccgagatcg acgaactgcg cgtaccaacc
     1981 cagcaggtcc atgcgatgcc ggccctcggt gtccacctcg agcggctcga acagactcat
     2041 ctccgccggg aacagcagcg cgacttgctt cgtccgcggt tgatccgcga tcagcggcat
     2101 cacttcgctc gcccagtgca gccccgcgcg cagcgagtcc ttgaacggct gatccatgcg
     2161 gaacatcacg ccgccgtcgt ccaatccgct gtagccgtaa atgtgcaggc cggtcgcgcc
     2221 gttggcgatg agcgttgcga tcgcctcggc cggcgggacg accgagtaga tatccccgct
     2281 gacgtgccgg cccaggtaaa gcccgcccgt gaacggccgg gcgcgatttg cgctgcgcat
     2341 gatcgacgcc tcgacgctca gcgccatcgc gtcagcgcga ttctccgcat ttaatggcga
     2401 cgcaatatac agcacgccat cgacgtgctc ggcgcagcgg tacacgtcca gcgcccgctg
     2461 cccggtctgc cagcccgtgt gccccggcgg attgaagaaa tatccccact gatgcagcac
     2521 cggctcgata aacagcggcg tttccagctc gcgccagtgc tgcttcatcg tccgcagata
     2581 ttcatccagc tcatcggcca gatacgtttg attgtcgatc caacgatgaa agtccggcgt
     2641 gcgattggcg aagtccgcgg cgctcggccg cgcgcagccg acccagttca gctcctccgg
     2701 gcgcagccaa tactgatccg gccgcaactg ctcgaatgat tttgggttga gttgatagcg
     2761 ctggttcagc ttcgcgatat cgccgccata tcggcgctgc agccacgcga gatagcgctg
     2821 ccgtccctca tcgtcaaagc tgggtttggg aatcggctcg aacatcgtct gcatgacgat
     2881 cgcttcgctc gaaccaacgg ttcggcggcg catgcccttg ccgtacagat tcagcagccc
     2941 gcggacgtgc tcgaccatcg ccgcctgcgc gcgcggcgac caatatttgt acgtccccat
     3001 cggccggccg tcggggcaga tgctttcttc gccacgcaca ggcggcacat cgcggatcgt
     3061 cggatagaga ttgtcgccgc acaggtacag cgccaggtgc gtgtaatcga ggccgtgccg
     3121 ctcggcctcc tgcatcatga attcctgcgt cgcgacataa gggctggccg gctcgccacg
     3181 atatcgt
//
LOCUS       DATIMH010000551         1563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5621217, whole genome shotgun sequence.
ACCESSION   DATIMH010000551 DATIMH010000000
VERSION     DATIMH010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5621217"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..>1563)
                     /locus_tag="VLI90_09580"
     CDS             complement(58..>1563)
                     /locus_tag="VLI90_09580"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HSV14500.1"
                     /translation="GGFGITTSGDGGKGAYGGGGGGGAAGGGNGGSSDFGGGGGSGWA
                     GAFGGTNGGRGGFGGGGGAAADGYIEGGGNPGKGGHFGGHANQRQGGGGGGLGGAIFN
                     DSGTLLVENSTFYDNYAAHGFGGGYPSASRAADGGDAGGAIFSRNGSTTIVNATIASN
                     QGTGQGSGIVVYEDGEATSFVLQNTIIAKNNGANACFWTGNVSHGGVGNLIMNNGSGT
                     QPFGACDDPRVTTDPQLGLLQDNGGPTYTMAIPLFSSAMSAADPATSLPYDQRYADRP
                     QVGGYDIGAYEICRRFIVGLRLAPCSETNIPPPSTVTLTMQASPSNGGTTTPASGNNE
                     EQVNAVIPIVASPTYGYLFGGWSANVTDASNPATTVIMDSSKTVAANFTYCNCALDVT
                     SAIRVTRGGLTLNPVTGRYAQTITLINTSSNTIAGSISLVLDSLSSNAALFNLTGTTD
                     ALESPAGSPYANVTSSSLPAGGSVSFALQFTNPSHTVITYSTRVLAGPGSR"
BASE COUNT          246 a          523 c          500 g          294 t
ORIGIN      
        1 gaaccgactg ttgggcctca ttggaatccc ctttcggacg agtaacgcgt ggtacggcta
       61 tcgcgatccc ggacccgcaa gcacgcgcgt cgagtacgta atgaccgtat gggacggatt
      121 cgtgaattgc agagcaaacg aaacacttcc acccgccggc aagctgctgc tggtgacatt
      181 ggcgtacggg ctgccggccg gcgactccag cgcatccgtc gtgccggtca ggttgaacag
      241 cgcggcgttg ctgctcaggc tgtcgagcac cagcgagatc gagcccgcaa tggtgttgga
      301 cgaggtgttg atcagcgtaa tcgtctgggc gtagcgtccg gtcaccgggt tcagcgtcag
      361 acctccgcgc gtcaccctga tcgcgctcgt cacgtcgagc gcgcagttgc agtacgtgaa
      421 gttcgccgcg accgtcttgc tgctgtccat gatcaccgtc gtcgccggat tgctcgcgtc
      481 ggtcacgttc gcgctccaac cgccgaacag gtacccgtag gttggtgaag cgacgatggg
      541 aatgaccgcg ttcacctgtt cttcgttgtt cccggacgcc ggcgtggtcg tgccgccgtt
      601 cgatggcgaa gcctgcatcg tgagcgtcac cgtcgacgga ggcggaatat tcgtctcgct
      661 gcacggcgcg aggcgcaagc ccacgataaa cctgcggcag atctcgtagg cgccgatgtc
      721 gtagccgcct acttgcgggc ggtcggcgta acgctggtca tagggaagcg acgtagccgg
      781 atccgctgcg ctcatcgccg aactgaagag cgggatggcc atcgtgtacg tcgggccacc
      841 gttgtcctgc agcagtccaa gttggggatc ggtcgtgacg cgagggtcgt cgcatgcacc
      901 aaatggctgc gttccggagc cgttgttcat gatcaggttg ccgactccgc catggctgac
      961 gttgccggtc cagaagcagg cgttggcgcc gttgttcttc gcaatgatcg tgttctgcag
     1021 cacgaacgat gtcgcctcgc catcctcgta gacgacgatg cccgatcctt ggccggtgcc
     1081 ttgattgctc gcgatagtcg cgttgacgat cgtcgtcgag ccgttgcgcg agaagatcgc
     1141 gccgccggca tcgccgccat ccgccgcgcg gcttgcgctc ggatagccgc cgccgaagcc
     1201 gtgcgcggcg tagttgtcgt agaacgtgct gttctcgacc aagagcgtgc cgctgtcgtt
     1261 gaagatcgcg ccgcccagtc cgccgccgcc gccgccttgg cgctggttgg cgtgaccgcc
     1321 gaagtgaccg cctttgccgg gattgccgcc gccctcgatg tatccatcgg ccgcagcacc
     1381 accgccgccg ccaaagccac cgcggccccc gttcgttcca ccgaatgcgc cggcccaacc
     1441 ggagccgccg ccaccgccga aatcgctgga gccgccgttg ccaccaccgg cagcgccacc
     1501 gccgccgccg ccgccgtatg cgcctttgcc gccgtcgccg ctagtcgtta tgccaaagcc
     1561 gcc
//
LOCUS       DATIMH010000552         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3022890, whole genome shotgun sequence.
ACCESSION   DATIMH010000552 DATIMH010000000
VERSION     DATIMH010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022890"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2076)
                     /locus_tag="VLI90_09585"
     CDS             complement(<1..>2076)
                     /locus_tag="VLI90_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV14501.1"
                     /translation="QKLENAKGVGQITILGGARRELHVLVDPDRLRSYNLTITDVFNA
                     VRAQNLELPGGNLNAGAKEFTVRTTGRVPDASQFNQITVANRNGYIVKVGDIGIAEDS
                     YEEPRTAARLDGVPAVTLVVAKQSGENTVTTAAEVQQRLKTIQATLPKDVRTQVVSDQ
                     SVFIKAAVENIKRHLIEGSFFAAIIIFLFLANIRTTLIAAVAIPTSIISTFALMAAMG
                     FTLNQITMLALTLMVGIVIDDAIIVLENIYRFIEEKNMPPFQAAIQGTKEIGLAVMAT
                     TLSLLAVFLPVGFMGGIVGRFMSSFGFTSAFAIAVSLLVSFTLTPMLTSRFVKRPKHA
                     KGEHSSKESFLFRHLDGWYTRMLHWSMQHRKAVVALSVLVVMSIVPLFMLVGKNFLPQ
                     DDQSQYNVLVRTPEGTSLAATAAVTERIAQEIRKLPGVTHTLATVGGGADRSVNNAAV
                     YVKLSDIDQRHITQQQLMQRTRDLLKSFPQEIHTGVELVSTIGGNQSNADVQYFIQGP
                     DLQKLGKYSEALLAKMRTIPGLADADSTLRSGKPEVRLEIDRARAGDLGVSVLDIEQA
                     LNTLVAGQVASTFNAGDDQYDVRVRAQEQFRGSSEGLTKMTVPSMKLGSVPLDQVAHI
                     IPGAGPSSINRINRQRQVTVTGNVLPGGSQSDILAKLNEYRDQLGMDPDYRSGLAGAS
                     KELGRTGFYF"
BASE COUNT          371 a          706 c          589 g          410 t
ORIGIN      
        1 gaagtagaat ccggtgcgcc cgagctcttt cgacgcgccc gccaggccgg agcggtagtc
       61 tggatccatc ccgagctggt cacgatactc gttcagtttc gcgaggatat cggactgcga
      121 cccgccgggt agcacgttgc ccgtcaccgt cacctgccgc tgccggttga tgcgattgat
      181 tgacgacggc cccgcccccg gaatgatgtg cgccacctgg tcgagcggca ccgacccgag
      241 tttcatcgag ggcaccgtca tcttcgtaag accctcggag ctgccgcgga actgctcttg
      301 cgcgcggacg cgcacgtcat actgatcgtc gcccgcgtta aacgtcgatg ccacctgccc
      361 cgcgaccaac gtattcagag cttgctcgat gtcgagcacc gacacgccca gatcgccggc
      421 ccgcgcgcgg tcgatctcca gccggacctc cggcttgccg ctgcgcaacg tgctgtccgc
      481 atcggccagt cccggaatcg tccgcatctt tgccagcagc gcctccgagt acttgccgag
      541 cttctgcaaa tccggaccct ggatgaagta ctgcacatcc gcattgctct ggttgccgcc
      601 gattgtgctc accagctcga cgccggtatg aatctcctgc gggaacgatt tcaacaggtc
      661 gcgtgtgcgc tgcatcagct gctgctgcgt gatgtgccgc tgatcgatgt cgctcagctt
      721 cacgtacacc gccgcattat taaccgaccg gtccgcgcca ccgcccactg tcgccagcgt
      781 atgcgtgacg cccggcagct tgcggatctc ctgcgcgatg cgctccgtta cagcggccgt
      841 cgccgccaga gacgtgcctt ccggcgtccg caccagcacg ttgtattgcg attgatcgtc
      901 ctgcggcagg aagttcttgc cgaccagcat gaacagcggc acgatcgaca tcactaccag
      961 cacgctcagc gcgactaccg ccttcctgtg ctgcatcgac cagtggagca tccgcgtgta
     1021 ccatccatcc aagtgacgga acaggaagga ttctttcgac gaatgctccc ccttcgcgtg
     1081 cttcggccgc ttcacgaagc ggctcgtcag catcggcgtc agtgtgaacg acaccagcag
     1141 cgaaaccgca atcgcgaacg ccgacgtgaa cccgaacgag ctcataaacc ggcctacgat
     1201 gccccccatg aagcccactg gcagaaacac agccagcagc gacaatgtcg tcgccatcac
     1261 cgccaggccg atttctttcg tgccctgaat cgccgcctgg aacggcggca tgttcttctc
     1321 ttcgatgaac cggtaaatgt tttcgagtac gatgatcgcg tcgtcgatca cgatgccgac
     1381 catcagtgtg agcgcgagca tcgtgatctg gttcagcgtg aaccccatcg ccgccatcag
     1441 cgcgaaggtc gagatgatgg acgtcggaat ggcgaccgcc gcgattagcg tcgtccggat
     1501 attcgccagg aacaggaaaa tgataatcgc cgcgaagaag ctgccctcga tcaggtgccg
     1561 cttgatgttc tcgaccgccg ctttgataaa tacggactgg tcgctgacga cctgagtccg
     1621 gacgtccttc ggcagtgtcg cctggatcgt cttcagccgc tgctggactt ccgcggccgt
     1681 cgttacggtg ttttcgcccg attgcttcgc gaccaccagc gtgaccgccg gaacgccgtc
     1741 cagccgcgcc gcggtgcgcg gctcctcata gctgtcctct gcgatgccga tatcgccgac
     1801 cttcacgatg tagccgttgc gattggccac cgtgatctga ttgaactgcg acgcgtccgg
     1861 cacgcgaccc gtggtccgca ccgtgaactc cttcgcgccg gcgttcagat tcccaccggg
     1921 cagctccaga ttctgcgcgc gcacagcgtt gaacacgtct gtgatcgtca gattgtaaga
     1981 gcgcagccgg tcgggatcga ccagcacgtg caactcgcgg cgggcgccac ccagtatcgt
     2041 gatctgcccc acgcctttcg cgttctcgag cttctg
//
LOCUS       DATIMH010000553         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2731590, whole genome shotgun sequence.
ACCESSION   DATIMH010000553 DATIMH010000000
VERSION     DATIMH010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2731590"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VLI90_09590"
     CDS             complement(<1..626)
                     /locus_tag="VLI90_09590"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HSV14502.1"
                     /translation="MPREQMESTDVAVIGAGPIGLELAVALKRAGVDYLHFDSKQIGY
                     TISWFAPQTRFFSSNERIAIAGVPLQTWDQGKATREEYLAYLRRIVQQFDLQVRTYEP
                     VTNIERAGDEFVLTSKPSSGERRIRAKRIVLCTGGTDHPRWLNVPGEDLPHVNHYFQD
                     PHTYFRKRLLIVGGKNSAVEAALRCYHAGADVSISYRRDQLNQSSIKY"
     gene            complement(761..>2261)
                     /gene="mutL"
                     /locus_tag="VLI90_09595"
     CDS             complement(761..>2261)
                     /gene="mutL"
                     /locus_tag="VLI90_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HSV14503.1"
                     /translation="TADADAAYEILNRGGAISDAQAAAGNVGTTVEVRNLFFNTPARR
                     KFIKGAATEFGHISEMLLRLALPYPHVAFRLSHNGRKALDLPATSAEERLLAAWPDDF
                     RELRLPIDYRDAEIRLRGLVGLPELARPTGKYQFLYLNGRHIRDKFIQHALREAFRGL
                     TEPGRYPAAVLLLELPAQDVDVNVHPTKIEVRFRDSGRIHGLVMAGIRERLLGSDLTP
                     TAIPIRSDAVEAPRVEMREKLAEFFRQMPSEYAATVSAPALSPSPGTPGEGRGEGDFE
                     RRSTFEIPNHPHPNPLPEYRARGPELTPLALPSAPAIQLHNSYLVAQSDDGLVIIDQH
                     ALHERIMYEELLARVTRGPLESQRFLIPHVVSVSSRQLALLDQIRPLLERLGIEVTPF
                     GPDSVGVQAFPSFLEKLDAGTFVRELLERGEQELLDMHGEELLHEILDMMACKAAVKA
                     GDPLTPAEIEALLARRALVERSSNCPHGRPTTLRLSLRDLEKQFKRTGF"
BASE COUNT          387 a          725 c          722 g          427 t
ORIGIN      
        1 cagtacttga tgctcgactg gttcagctga tcgcggcgat agctgatcga cacatccgcc
       61 cccgcgtgat agcagcgcag cgccgcttcg accgcggagt tcttcccgcc gacgatcagc
      121 agccgcttgc ggaagtacgt gtgcgggtcc tgaaaatagt ggttgacatg cggcagatct
      181 tcgccgggca cgttcagcca ccgcgggtga tcggtgccgc cggtgcacag gacgatgcgc
      241 ttggcgcgga tgcgccgctc gccgctggag ggtttgctcg tcagcacaaa ttcatcgccg
      301 gcgcgttcga tgttcgtcac cggctcatac gtgcggacct gcaggtcgaa ctgctgcacg
      361 atccgccgga ggtacgcgag atattcttcg cgcgtcgcct tgccttggtc ccacgtctgc
      421 agcggcacgc cggcgattgc gatgcgttcg ttgctgctga agaaccgcgt ctgtggtgcg
      481 aaccacgaga tcgtgtagcc gatttgcttg gagtcgaagt ggaggtaatc gacgcccgcg
      541 cgcttgagcg cgaccgccag ctccagccca atcggccctg cgccaatcac cgcgacgtcc
      601 gtagattcca tctgctcacg cggcatgcgt tgcaactata ggcaaggaca aggagaggat
      661 taaccacaga tacacacaga tgcacacaga taaactcaga taggattatt ttgtctgtct
      721 ttatccgtgt gcatctgtgt gcatctgtgg ttccatctct tcagaatccg gtccgtttga
      781 actgcttttc cagatcgcgc agcgacagcc gcagcgtggt tggccggccg tgcgggcagt
      841 tgctcgaccg ttccaccaga gcccggcggg cgagcagggc ttcgatctcc gccggggtca
      901 gcgggtcgcc ggctttcacg gcggccttgc acgccatcat gtccagtatc tcgtgcagca
      961 gctcttcacc gtgcatgtcg agcagttcct gctcgccgcg ttcgagcagc tcgcgcacga
     1021 atgtgccggc gtcgagcttt tccaggaacg acgggaacgc ctgcacgccg accgagtccg
     1081 ggccgaacgg cgtgacttcg atgccgagcc gctcgagcag cgggcggatc tggtcgagca
     1141 gcgccagctg ccggctcgac acgctgacga catgcgggat caaaaaccgc tgcgattcca
     1201 gcggcccgcg ggtcacgcgc gccagcagct cttcgtacat gatccgctcg tgcaacgcgt
     1261 gctggtcgat gatgaccagg ccatcgtccg actgcgcgac gaggtagctg ttgtgaagtt
     1321 ggatggcggg cgcgcttgga agcgccaatg gcgtcaactc cggtcccctc gcccggtact
     1381 ccgggagagg gttagggtga gggtgatttg gaatttcgaa agtcgatcgg cgctcgaaat
     1441 caccctcacc ccgaccctct cccggtgtac cgggcgaggg ggacagagcg ggcgccgaaa
     1501 ccgtcgccgc gtactccgac ggcatctgcc ggaagaactc cgccagcttc tcgcgcatct
     1561 ccacgcgcgg cgcttcgacc gcatctgatc gaattggaat cgccgtcggc gtgagatcgc
     1621 tgcccagcaa ccgctcgcgg atgccggcca tcaccagccc gtgaatccgc ccgctgtcgc
     1681 ggaagcgcac ctcgatcttc gtcgggtgca cgttcacatc cacatcctgc gccggcagct
     1741 ccagcagcag caccgcggcg ggataccgcc cgggttcggt caacccgcgg aatgcctcgc
     1801 gcagcgcgtg ctggatgaac ttgtcgcgga tgtgccggcc gttcaggtaa aggaactggt
     1861 atttgcctgt cggtcgcgcg agttccggca aaccgacgag gccacgcagg cggatctccg
     1921 cgtcgcgata atcgatcggc aatcgcagct cgcgaaaatc gtccggccac gctgcgagca
     1981 accgctcctc cgccgaggtc gcgggcaggt ccagcgcctt gcggccgttg tgcgacaggc
     2041 gaaaggcgac gtgcggatac ggcagcgcga gccgcagcag catctccgag atgtgtccga
     2101 attccgtggc agcgcccttg atgaactttc gccgcgccgg cgtgttgaag aacaggttgc
     2161 ggacttcgac ggtcgtgccg acgttccccg cggcggcttg ggcgtcgctg atcgctccac
     2221 cgcggtttag gatttcatat gcggcgtcgg catcggctgt g
//
LOCUS       DATIMH010000554         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4758065, whole genome shotgun sequence.
ACCESSION   DATIMH010000554 DATIMH010000000
VERSION     DATIMH010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4758065"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VLI90_09600"
     CDS             complement(<1..1356)
                     /locus_tag="VLI90_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HSV14504.1"
                     /translation="MATTNPFGSESTLSSKAGDVKIYRLKKLAELGIGNVDQLPISIK
                     VLLESALRNMDDFEVTEEDVQGLANYNAEKVKAVELPFKPARVILQDFTGVPAVVDLA
                     AMRSAMKRAGGDPQRINPLVPVDLVIDHSVQVDEFGSKLSLFHNTEKEFERNRERYEF
                     LKWGQKAFKNFSVVPPSIGIVHQVNLEYLAKVVMVRDNVALPDSLVGTDSHTTMINGL
                     GVVGWGVGGIEAEACMLGQPIYMVTPQVVGFKLHGKLPEGSTATDLVLTITQILRSHG
                     VVEKFVEFFGEGLANMGVADRATIANMAPEYGATIGYFPIDERTLSYLRLTGRDENHI
                     DLVERYAKEQGLWLQTKAEPKFAEVLELDLGTITPSVAGPRRPQDRIELRGVKQNFRS
                     TLVDVFKKEVGESATPRLDRWSAEAPVTPATMTEAEGHGGEDEKEAATAEHIKSRPES
                     AI"
     gene            1586..>3503
                     /locus_tag="VLI90_09605"
     CDS             1586..>3503
                     /locus_tag="VLI90_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HSV14505.1"
                     /translation="MSYRRRMFRRSVATAVESLERRTLFTATFTYADVAAFDLNSGAF
                     PQGSLVMDKDGNIFGTASRGGDADPLSLAGGDGAIFERVKSTNQIIAIATFDGSNGRE
                     PTGLTMDGNGDLFGVTREGGDAQGDGTVFKVALSQGSNEIQTLALFDSTTGIKPNGNL
                     FIDTNGNVFGTTPQGGISGSDVGSGTVWELASGASTITTLATFDPANGGGANPNSNVV
                     FDSSGKLYVTTTNGGPTGNGTVSTVQNGQPVVLASFNLSNGRAPTGNTAIDANGNIFG
                     AASGGGDNSEGNVWELPAGGDTIQVLASFDGIDNNQTGAEPESGVTLDANGNLFGTAT
                     NGGANGAGTIWELPSGVNFITAIVQLSDQGSTATDGPLLTDTHGNLFGLSRTGGANDL
                     GKLYELTTSEPINPIHLAFDQQPTDTTIIDSITPPVTVQVLDADNNLVTAENAVTMSI
                     ASGPAGAVLGGTTMIAVIDGIATFDKLALNTPGTYTLFASDGAGTTPATSDPFDVTFP
                     GGGNGAITAAFQRTTLPATFVPGDRGVAVFQLQNAGNTLARGVVRVQMFLSPTGQMID
                     AVPVATPAALANLPVILAAGRQRPLVAPFSIPQNIAVGNEQLLVTLTPVGGFASSSVN
                     TQPIVDANVEQAALN"
BASE COUNT          653 a         1181 c         1040 g          629 t
ORIGIN      
        1 gatggccgat tcggggcgac tcttaatgtg ctccgccgtc gccgcttcct tttcgtcttc
       61 gccaccgtgg ccttccgcct ccgtcatcgt cgccggcgtc accggggctt cagcgctcca
      121 gcggtccaac cgcggcgtgg cgctttcgcc gacttccttc ttgaaaacat ccaccagcgt
      181 cgagcggaag ttctgcttca cgccgcgcag ctcgatgcga tcctgcggcc ggcgcggccc
      241 ggcaacgctc ggcgtgattg tgcccaggtc cagttccagc acttcggcga acttcggttc
      301 ggccttcgtc tgcagccaca ggccttgttc cttcgcgtaa cgctcgacca gatcgatgtg
      361 attctcatcc cggccggtca ggcgcagata gctcagcgtg cgctcgtcga tcgggaaata
      421 tccgatcgtc gcgccatatt ccggcgccat gttggcgatc gtcgcgcggt cggccacgcc
      481 catgttggcc agaccctcgc cgaagaactc gacgaacttt tcgacgacgc catggctgcg
      541 gaggatctgc gtgatcgtca gcaccaggtc cgtcgccgtc gatccttccg gcaacttgcc
      601 gtgcagctta aatcccacga cctgcggcgt gaccatgtaa atcggctggc cgagcatgca
      661 cgcctcggct tcgatcccgc ccacgcccca accgacgaca ccgaggccgt tgatcatcgt
      721 cgtgtgactg tcggtgccga cgagtgaatc cggcagcgca acgttgtcgc gcaccatcac
      781 gaccttcgcc agatactcga gattcacctg atgcacgatg ccgatgctcg gcggcaccac
      841 ggaaaaattt ttgaacgcct tctgtcccca cttcagaaac tcataccgct cgcgattgcg
      901 ctcgaactct ttctcggtgt tgtggaacag cgacaacttc gatccgaatt cgtccacctg
      961 caccgagtga tcgatgacga gatcgaccgg cacgagcggg ttgatgcgct gcggatcccc
     1021 gccggcgcgc ttcatcgcgc tgcgcatcgc cgccaagtcg acgacggcgg gcacgccggt
     1081 gaagtcctgc aggatcacgc gggccggctt gaacggcagc tcgaccgcct tcaccttctc
     1141 ggcgttgtaa ttcgccaggc cctgcacgtc ctcttcggtc acctcgaagt catccatgtt
     1201 gcgcagcgcg ctctccagca gcaccttgat ggaaatcggc agctgatcga cgttgccgat
     1261 gcccagttca gcaagcttct tcaggcgata gatcttcaca tcgccggctt tggaggagag
     1321 cgtggattcg gaaccgaaag ggttagttgt ggccatatcc gtggttgaaa aagggactca
     1381 tcgtgcggaa acccagcgaa agcgaatgat cttatgcgcg gggggtaggg gtaacaagga
     1441 cgggaattgc agaacccgac gttgagcgcc catgcgagca tgaacgtggc cgcgcgccga
     1501 tgcgtccttg acggagacca tcgcattccc gcacgatacc gccgcacgcc gtttgtaccc
     1561 acgttgtact ccgaggaggg accgcatgtc gtatcgccgc cggatgttcc gccgctccgt
     1621 tgccacggct gttgagtcac tcgaacgtcg aacgctgttc accgccacgt ttacctatgc
     1681 cgatgtggcc gcgttcgact tgaactccgg cgcatttcca cagggatcgc tggtgatgga
     1741 caaggacggc aacatcttcg gcaccgccag ccgggggggc gatgcggacc cgttgtcgct
     1801 cgccggtggc gatggcgcga tcttcgaacg ggtgaagtcg accaatcaga tcatcgccat
     1861 cgcgacgttc gatgggtcca acggccgcga gccaactggt ttgacgatgg acggcaacgg
     1921 cgatctgttc ggcgtcacgc gcgaaggtgg tgacgctcaa ggcgatggca cggtgttcaa
     1981 ggtcgcgctc agccagggga gcaacgagat tcaaacgctc gcgctgttcg attccacgac
     2041 cggcatcaag ccgaatggga atctgtttat cgacacaaac ggcaacgtgt tcggcaccac
     2101 gccgcagggc gggatttccg gcagcgacgt cggctcgggt accgtttggg aattggccag
     2161 tggcgcatcg acgatcacca cgctggcaac gttcgatccc gccaacggcg gcggcgcgaa
     2221 tccgaacagc aacgtcgtct tcgacagcag cgggaaactc tacgtcacga cgacgaacgg
     2281 cggccccacc ggcaacggca cggtctcgac cgttcagaac ggtcagccgg tcgtgctggc
     2341 cagcttcaac ctgagcaacg gccgcgcgcc caccggcaac accgcgatcg acgccaacgg
     2401 caacatcttc ggcgccgcca gcggcggtgg cgataactct gaaggcaacg tgtgggaact
     2461 gcccgccggt ggcgacacca ttcaggtgct cgcgtcgttc gatggcatcg acaacaacca
     2521 gactggcgcc gagcccgaaa gcggcgtcac gctcgacgcc aacggaaacc tcttcggcac
     2581 cgcgaccaac ggcggcgcca acggcgccgg caccatctgg gaactgccca gcggtgtgaa
     2641 cttcatcacg gccatcgttc agctgagcga tcaaggcagc accgcaaccg atggtccgct
     2701 gctgaccgac acgcacggca atctctttgg actgtcgcgc accggtggcg cgaacgatct
     2761 gggcaagctg tatgagctga ctacgtcgga gccgatcaat ccgatccacc tggcgtttga
     2821 ccagcagccg accgacacca cgatcatcga ctcgatcacc ccgccggtga cggtgcaggt
     2881 gctggatgcc gacaacaacc tggtgaccgc ggaaaacgcc gtgacgatgt cgatcgccag
     2941 cggacccgct ggcgccgtgc tcggcggcac gacgatgatc gccgtcatcg acggcatcgc
     3001 cacattcgac aagctcgcgc tcaacacgcc cggcacgtac acgctcttcg cgtccgacgg
     3061 cgccggcacc acaccggcca cgtccgatcc gttcgacgtc acgttccccg gcggtggtaa
     3121 cggcgcgatc acggcggcgt ttcagcgcac tacgttgccg gccaccttcg tccccggtga
     3181 ccgcggcgtc gccgttttcc aattgcaaaa cgccggcaac acgctggcgc gcggcgtcgt
     3241 ccgcgtgcag atgttcctat cccccactgg ccaaatgatc gatgccgtac ccgtcgccac
     3301 gccggcagcg ctcgccaatc taccggtcat cctcgccgct ggacgacagc ggccgctggt
     3361 cgcgccgttt agcattccgc agaacatcgc ggtcggcaac gagcagttgc tcgtcacgct
     3421 cacgcccgtc ggcggcttcg cgtccagcag cgtcaacacg cagccaatcg tcgatgcgaa
     3481 cgtcgaacaa gcagcgctga att
//
LOCUS       DATIMH010000555         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_134608, whole genome shotgun sequence.
ACCESSION   DATIMH010000555 DATIMH010000000
VERSION     DATIMH010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134608"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VLI90_09610"
     CDS             complement(<1..256)
                     /locus_tag="VLI90_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14506.1"
                     /translation="MALIVTAQHSEVKINDETVEGLQSIDYSLVKNRSQVGAIGTDER
                     IAVYFGIKSVVGKLRVASVNATLDGLLSSNAEFSVSATLTH"
     gene            complement(288..2576)
                     /locus_tag="VLI90_09615"
     CDS             complement(288..2576)
                     /locus_tag="VLI90_09615"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HSV14507.1"
                     /translation="MPSALIIPGVQVKTEFEPSPVLPGATGILGVVGVADRGPVTPTQ
                     VGNFAEFIDIFGPASRYTMPEVRGAFANGVSRVVIARTSPGRGGKASLVLKDDDGEDV
                     VKIEARAEGAWANKLSVQATQVRGLSGAGVKFVNLAVKLNGQVVETFNGLVTDEDSPD
                     YLFDRINFSSRTIIAVDPKFEKGLPIDQIATPLADAEARSAKASLKAGGADVILVEAK
                     RNGKGGNQAAVLVEEGQAGLTLTGPANAASLDIKARVPGAAGRSIRLSVTANPDATVN
                     VTVTPAAGAPRVLGPFTTLDDLVNRLKTDPDVTADALGAALPTAIASTPLARRVNVSV
                     IAEGRDTQVYSHLADLAAVAAINDPTVKLTVVPGATALPDVNTGVNLTGGRGKGASLA
                     LVGDGGDDPLLELVPASGVKGALAVAVQRGVSTIDNATGVVALTITVDGDIAETFPDL
                     TMDPDDPNFLPEVLVSSSALIRAHDLIVRSRTTSLPKHMSKPANLTGGTSPSTDDYQD
                     ALDRLEGEESVDLVIASVANQLTDAEVVTVHQEVTAHCTKMADVARNRIGLGSPSISE
                     TNDVNKILDHANSVRSDYFIFTAPAGMEAAVAGLLGRQDYFQSPTFKTIAAPTAPLVR
                     YSDSQLEKLITGNVLAVNEKRNLGIIVIKGLLTSGRQVNVQRTANKAVRDVKAISDKY
                     IGLLNNAGTRNALRQQIFAMFLQMERDGALVPSVDGKDPAFAVDVYSTQADFANGIVR
                     IDIAIRPVRAIDYIYATILVKN"
     gene            complement(2805..>3802)
                     /locus_tag="VLI90_09620"
     CDS             complement(2805..>3802)
                     /locus_tag="VLI90_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSV14508.1"
                     /translation="FADVSENSGIARVKAGYALTAVATDLDGDGWPDIYVACDTSPSL
                     LFHNNHDGTFTERGLEAGVALNEDGREQGGMGLGIGDFDNDGHLDIFKTHFSADTHAL
                     YRNNGKGTFRDVTTRAGLAVETRFVGWGAAIQDFDNDGLPDIFFTTGMVYPEVESHEP
                     DSPFKTPNVLFRNLGNGKFEELLDQAGPAMKEVHSSRGAAFGDFDNDGDIDVLIMNIN
                     EPPSLLRNDVSGTNHWLKVKLEGVASNRSAIGATVIVTYGERRQAQAVLAQTSYLSAN
                     DRRLHFGLGGESAAAIEIHWPNGRREKIEDVAANQLVIIREGAGIIRRETFKARP"
BASE COUNT          673 a         1181 c         1157 g          791 t
ORIGIN      
        1 cgtgcgtcag ggtggcgctc accgagaact ccgcgttgga gctgagcagg ccgtcgagcg
       61 tcgcattcac cgacgcgacg cgcagctttc cgacgacact cttgattccg aaatacacgg
      121 cgattcgctc gtcggtgccg atcgcgccaa cttggctgcg gttcttcacc aggctgtaat
      181 cgatagattg cagcccttcc actgtctcgt cattgatctt aacttccgaa tgctgggcag
      241 tgactatcag cgccattcct gcttctcctt ttgagcccgg gctcagccta gttcttcacc
      301 aaaatcgtgg cgtagatata atcgatggcg cgcaccggcc gtatagcgat gtcgatgcgc
      361 acgatgccgt tcgcgaaatc ggcctgcgtc gaatacacat ccacagcgaa tgccggatcc
      421 ttgccatcga cactcggcac cagcgcgccg tcgcgctcca tctgtaggaa catcgcgaag
      481 atctgctggc gcagcgcgtt gcgcgtaccc gcgttgttga gcagtccgat gtacttgtcg
      541 gagatcgcct tcacatcgcg caccgctttg ttggccgtgc gctgcacgtt cacctggcgg
      601 ccgctggtga gcagcccttt gatgacgata attcccaaat tgcgtttttc gttgactgcg
      661 agcacattgc ccgtgatcag cttttcgagt tgagaatccg agtagcgcac cagcggcgca
      721 gtgggcgcgg caatcgtctt gaaggtcggc gactggaaat aatcctgccg tcccaagagt
      781 ccggctacag ccgcttccat gccggccgga gctgtgaaga tgaagtaatc gctgcgcacg
      841 ctgttggcgt ggtcgaggat cttatttacg tcgttagtct ccgagattga gggcgagccc
      901 aggccgatgc gattgcgcgc cacatccgcc atcttggtgc agtgcgccgt cacctcctga
      961 tgcacggtga cgacttccgc atcggtgagt tgattggcca ccgatgcgat caccaggtcc
     1021 accgattcct cgccttcgag acggtccagc gcatcctggt aatcgtcggt ggaaggcgac
     1081 gtgccgcccg tgagattggc cggcttcgac atgtgcttgg gcagcgatgt ggtccggctg
     1141 cgcacgatca gatcatgcgc gcggatcagc gccgatgacg agaccagcac ttccggcagg
     1201 aagttcggat cgtccgggtc catggtcagg tccgggaaag tctccgcgat atcgccatcg
     1261 acggtgatgg tcagcgccac tacgcccgtc gcgttatcga tggtggaaac gccgcgctga
     1321 actgccacgg ccagcgcgcc cttcacgccg ctcgccggga cgagttccag cagcgggtcg
     1381 tcaccgccgt cgcccaccag agctaaggat gcgcctttcc cacgcccgcc cgtcagattc
     1441 acaccggtgt tcacgtcggg cagcgcggtg gcgcccggga ccacggtgag cttcactgtg
     1501 gggtcgttga ttgcggccac agcggcgagg tcggcgaggt gcgagtacac ctgggtatcg
     1561 cgtccttcgg caatcaccga tacattcacg cgccgcgcca ggggcgtcga ggcaatggcc
     1621 gtgggcaacg ccgcgccgag cgcatcggcc gtcacatcgg gatccgtctt caggcgattc
     1681 accagatcgt cgagcgtagt gaacggaccc agcacgcgcg gcgcgccggc cgccggcgtc
     1741 accgtcacgt tcacggtggc atcgggattg gccgtcacgg aaagtcgtat gctccgtccg
     1801 gcagcgccag gcacgcgcgc cttgatatcc aagctggcgg cattcgccgg gccggtgagc
     1861 gtcaagcccg cctgaccttc ctccaccaac accgccgcct gatttccgcc cttaccattg
     1921 cgcttggctt ctaccaggat cacgtcggcg ccgccggctt tcagggatgc cttcgcggag
     1981 cgcgcctccg catcggcaag cggcgtggcg atctggtcga taggcaggcc cttttcgaat
     2041 ttcgggtcca ccgcgatgat ggtgcgcgaa ctgaaattga tgcgatcgaa gaggtagtcg
     2101 gggctgtctt cgtccgtcac gaggccgttg aaagtctcca ccacctgacc gttcaacttc
     2161 accgcgaggt taacgaactt gacgcccgcg ccgcttagtc cgcgaacttg tgtggcctgg
     2221 acggaaagct tgttggccca cgcgccttcc gcgcgcgctt cgatcttgac gacgtcttcg
     2281 ccatcgtcgt ctttgagcac caggctggct ttaccgcccc tgcccggcga ggtccgagcg
     2341 attacaacgc gcgacacgcc attggcgaac gcgcctcgca cttctggcat ggtgtagcgg
     2401 cttgcgggtc caaagatgtc gatgaattca gcgaaattcc caacctgtgt gggtgtcacc
     2461 ggaccgcgat ctgcgacgcc cactaccccg aggatcccag tggctcccgg gagcaccggc
     2521 gagggttcaa actcagtttt gacctggaca ccagggatga tcaaagcact cggcatcgct
     2581 cctcctcaac ctgcaaattt gttttgaaaa gcgacgacgc ggagctgttc tggactagta
     2641 cccctccagg atttcccggc cccccgccgt actaatctca gctgctggta aagtctgtca
     2701 cggtacgatt tgcttgtcaa tccggtattt gaagcacgct tcaaaacacg tactgtcgga
     2761 gttttcacgc ggtcaatagc gatctgagtg gcgagttcga acgcctatgg gcgagccttg
     2821 aaagtctctc tgcgaatgat gccagcgcct tcgcgtatga tcacgagttg gttagcggcc
     2881 acatcctcga ttttttcccg gcggccattc ggccagtgaa tctcgatcgc ggcggcggat
     2941 tcacctccca atccgaagtg gagccggcgg tcgttggccg aaagatagga cgtctgcgcc
     3001 agcacggcct gcgcctggcg ccgctcgccg taggtcacga tgaccgttgc tccgatcgcg
     3061 ctgcgattgg atgccacgcc ttccagcttt accttgagcc aatgattcgt gccgctcaca
     3121 tcattgcgca ggagcgaggg cggctcgtta atgttcatga tgagcacatc gatatcgcca
     3181 tcgttatcga aatcgccgaa ggccgctccg cggctcgagt ggacttcctt catcgcgggc
     3241 ccggcctgat cgagcagctc ctcgaatttc ccattgccga gattgcggaa tagaacgttc
     3301 ggagttttga aaggcgaatc gggttcatgg gattccacct cggggtagac cattccggtg
     3361 gtgaagaaga tgtcgggcag accgtcgttg tcgaaatcct gaatggccgc gccccagccg
     3421 acgaagcgcg tttcgacggc aagcccggcg cgggtagtca cgtcgcgaaa cgtgcccttg
     3481 ccgttgttcc ggtagagcgc gtgggtgtcc gcgctgaaat gagttttgaa gatatcgagg
     3541 tgaccgtcat tgtcgaaatc gccgatgcct agccccatgc cgccctgttc gcgcccgtcc
     3601 tcgttgagcg ctactccggc ttcgaggccg cgctcggtga aggtgccgtc gtggttattg
     3661 tggaagagca ggctgggaga ggtgtcgcag gcgacgtaga tgtcgggcca gccgtcgcca
     3721 tcgaggtcgg tcgcgacggc ggtgagggcg tacccggctt tgacgcgcgc gatgccggag
     3781 ttctcgctaa cgtccgcaaa gg
//
LOCUS       DATIMH010000556         5826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1002323, whole genome shotgun sequence.
ACCESSION   DATIMH010000556 DATIMH010000000
VERSION     DATIMH010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002323"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VLI90_09625"
     CDS             <1..1065
                     /locus_tag="VLI90_09625"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HSV14509.1"
                     /translation="KVTTPAAPALGAGDGAAGAYFNDEDDEHCAGMPTMTRVDPTIDF
                     DWGWRSPDRDHAIRPDHFSVRWTGKIQPQYSETYTFYLRSDDGARLWIDGKLLIDDWA
                     PEHGDHEQPGTIKLSAGQKYHFRVEYYEDAMDAEARLWWSSPSTMKQPVPKSQMYSGT
                     APAVPTELSVSAASNTQADLKWRDNASNETGYAVERKAGDEAEFIDIAKLSADVTSYH
                     DTGLNPAARYTYRVRAINFREDSAPSNTVSLTMPTPPQTPSDCRVTRVTSRSIHLAWE
                     DKSDNEDGYRILRRQSGGTFIAIATLPPNSTEFTDDGKDHAGLPSGTAFDYHVQAYNI
                     AGASDFTGATATTLGQDPAK"
     gene            1134..1340
                     /locus_tag="VLI90_09630"
     CDS             1134..1340
                     /locus_tag="VLI90_09630"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV14510.1"
                     /translation="MDELISQLTRRVTQQDYLRLEEASATKHEYRDGQIIDMAGGTFA
                     HACIAANLLRRLGEKLDDSPCEDP"
     gene            complement(1518..1790)
                     /locus_tag="VLI90_09635"
     CDS             complement(1518..1790)
                     /locus_tag="VLI90_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HSV14511.1"
                     /translation="MNISLPQPLKRYVDDKVASGIYSSGSEFVREAIREKLQRDERRN
                     QAEQALTEQLIAGLDSGKPISFTSGYFKAKKRALLQRAAAKKKKIA"
     gene            1910..2062
                     /locus_tag="VLI90_09640"
     CDS             1910..2062
                     /locus_tag="VLI90_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14512.1"
                     /translation="MIRGPFSEADRAIERFKTKLPKLYHGHTQAFCAVVIRAVKENPA
                     AFTAAA"
     gene            complement(2068..2793)
                     /locus_tag="VLI90_09645"
     CDS             complement(2068..2793)
                     /locus_tag="VLI90_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14513.1"
                     /translation="MSADGLRSPRCSEARRSSYLIDDALVRASSAGLRNIATIKVRVY
                     QHLITEVSADQIITPQERHVMAMTREMLRLSDADVGSLEQELNRREVIWSIQRGELPT
                     INSPVALQKNEVARFAFYGVEFHQERVVSRKCVGSSEGYSIRIAKGVTYRAGSHRGRY
                     VNRTALVPVGIGQLVLTSKRLIFAGTSGFSVPFIKILQLTAYADGFAITKDSTAKSNV
                     PFVFICPDPELTILATTACINGT"
     gene            complement(2985..3812)
                     /locus_tag="VLI90_09650"
     CDS             complement(2985..3812)
                     /locus_tag="VLI90_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14514.1"
                     /translation="MRWRALGADKSSGADRSITVEADSEKAALALANADGVLVSKLKR
                     LPDATGAHAATAEARIPLVETGPSTVPYASRVDPQDAWLKRIVQSRRTRFFAVGVLCI
                     ATIVVAAMLALKPRRFSRDEVLAGIGSQFVIDPKEVRSVDGTITQLGFSEDNLRSIRL
                     ETNGDHITYAHVTVMVYKPWMVGVLLHPETVGTALGTGTDAARSMLHYRELLDRFALN
                     VGGFKNDLEAKTWVDSSLKAIVTSPQDQVKLRRRGWSLTMVLDDPGGDLKLAMDTQK"
     gene            complement(3881..4132)
                     /locus_tag="VLI90_09655"
     CDS             complement(3881..4132)
                     /locus_tag="VLI90_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14515.1"
                     /translation="MKAIQIQSQIGSDGVLTPRVPLGAEEAAQRVLVMIAPIAETKAD
                     APRDETERHCFVEETYGSCAGLGLERPDQGEFERRDDLK"
     gene            complement(4168..>5826)
                     /gene="acnA"
                     /locus_tag="VLI90_09660"
     CDS             complement(4168..>5826)
                     /gene="acnA"
                     /locus_tag="VLI90_09660"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HSV14516.1"
                     /translation="FKKEVGESATPRLDRWSAEGPVAPFRAQAAQVQEHDTSDVTAVA
                     AGKEDEKEAATVEHLKGSTQKDAAFVAMDDDQWHKLTHGDVVIAAITSCTNTSNPSVM
                     IAAGLVAKKAVEKGLKVPPYVKTSLSPGSRVVTDYFNKSGLSEYLDKLGFQTTGYGCM
                     TCIGNSGPLPEPIANAIEKGDLVACSVLSGNRNFEGRINPHVRANYLASPPLVVAYAL
                     AGTMDLNIMDEPIQQNAKGEKVYLRDIWPTQQEVHDAIVASISPEMFQKQYGNVENGN
                     PEWNKIPVKGGELFNWDEHSTYIQEPPFFTNLGKEPAKVKPIIGARVLVMVGDSVTTD
                     HISPAGAIKKDGPAGRYLMEHGVQPIDFNSYGSRRGNDRVMTRGTFANIRLRNQLAPG
                     TEGGVTKYLPTGEVMSIYDASMKYQADGVPLIVLAGKDYGMGSSRDWAAKGVYLLGVN
                     AVIAESFERIHRSNLVGMGVLPLQFKRGQSRSSLGLTGVGTFDIQISDEISPLQDVQV
                     RVTSREGEPVEFTTICRIDTPVEIEYYRNGGILHTVLRNMLKKA"
BASE COUNT         1106 a         1843 c         1695 g         1182 t
ORIGIN      
        1 aaagtcacca cccccgccgc cccggcgctc ggcgctggtg acggggcggc cggcgcttac
       61 ttcaacgatg aagacgacga gcactgcgcc ggcatgccaa cgatgacgcg ggtcgatccg
      121 acgattgact tcgattgggg ctggcgctcg cccgatcggg atcacgcgat ccggccagat
      181 catttttcgg tgcgatggac cggcaagatc cagccgcagt acagcgagac atacacgttc
      241 tatctgcggt cggatgacgg cgcccggcta tggatcgacg ggaagctgct gatcgacgat
      301 tgggcgcccg agcacggcga ccacgagcag ccgggcacga tcaagctgtc ggccgggcag
      361 aaatatcatt tccgcgtcga gtattacgaa gacgcgatgg atgccgaggc ccggctgtgg
      421 tggtcgtcgc catcgacgat gaagcaaccg gtgccgaaat cgcagatgta cagcggcacc
      481 gcgccggcgg tgccgacgga gctgagcgtc tccgccgcgt cgaacacgca ggcggatttg
      541 aagtggcggg ataatgcgag caacgagacc ggatacgccg tcgaacgaaa ggccggcgac
      601 gaggctgagt tcatcgacat cgcgaagctg tcggctgatg tgacgagcta tcacgacacc
      661 ggcttgaacc ccgcggcgcg atacacatat cgcgtgcgcg cgatcaactt ccgcgaagat
      721 tccgcgccgt cgaacacggt gagcctgacc atgccaacgc cgccgcaaac gccgagcgat
      781 tgccgcgtga cgcgggtgac aagccggtcg attcacctgg cgtgggaaga caagtccgac
      841 aacgaagacg gctaccgcat cctgcgtcgc caatccggcg gcacgtttat cgcgatcgcc
      901 acgctgccgc cgaacagcac ggagttcaca gacgacggca aagaccacgc cggcctgccg
      961 agcgggacgg cgttcgatta tcacgtgcag gcgtacaaca tcgccggcgc ctcagacttc
     1021 accggcgcca cggcgacgac gctcggtcag gatccggcga agtgaatgtt cgcgatcgca
     1081 cggaatggga agcgcacgcg cgcgcaccac ggcatgtata atccggggct gttgtggacg
     1141 aattgatttc tcaactcacg cgccgcgtga cccagcaaga ttacctccgg ctggaggaag
     1201 cctccgccac caagcacgag tatcgtgacg gccagatcat cgacatggcc ggtggcacgt
     1261 tcgcgcacgc ctgcatcgcc gccaatcttc tgcgccggct cggcgagaag ctcgatgaca
     1321 gcccttgcga agatccatga ggcgtggatg gcccaattcg tagcgcggcc acctttcggg
     1381 atatatcctg atctgccgat atgtttcgtc caccgcgtcg taaaaccgca gcgccgcatc
     1441 gaggctgtcg cgcgcgatat actcggcagc ctcgtcgaca tctgcgtctg ccgcgggcag
     1501 gatccgcaga ccgtagttca tgcgatcttt ttcttcttcg ccgctgcgcg ttggagcagt
     1561 gcccgcttct tcgccttgaa atatcctgag gtgaacgaaa ttggcttacc gctgtcgagc
     1621 cccgcgatca actgctctgt cagcgcctgc tccgcctgat ttcgccgctc gtcgcgctga
     1681 agcttttcgc ggatcgcttc gcgaacgaac tcgctccccg agctgtagat gccggaagca
     1741 actttgtcgt ctacgtaccg cttgagcggc tgcgggagcg aaatgttcat cgtgacaatg
     1801 tgcggttcgg ccggcacgtc agcatcctaa cgccccgcca aaaattgaca agctttgctg
     1861 ctgctgcttc tgtcgatgtc agcagcgcct gcggctggaa gtttccgaat tgatccgcgg
     1921 ccccttctca gaagccgatc gcgcgatcga gcggttcaag accaagctgc ccaagctcta
     1981 tcacggccac acgcaggcgt tctgcgccgt cgtgatccgg gcggtgaagg agaaccccgc
     2041 ggcgttcacc gccgcggcgt gatccaatca agtgccgtta atgcacgccg ttgtcgcaag
     2101 aatcgtcagt tcaggatccg ggcagatgaa cacgaacggc acatttgatt ttgcggtcga
     2161 atctttcgtg atcgcaaagc cgtccgcata cgccgtcagc tgaaggatct tgataaacgg
     2221 gacgctgaac ccggacgtgc ccgcaaagat caatcgcttg ctcgtgagga cgagttggcc
     2281 gatgcccacc ggcaccagcg cggttcggtt cacgtatcgg ccgcggtggg aaccggcccg
     2341 atatgtaacg cccttggcga tgcgaatgct gtagccttcc gagcttccga cacatttgcg
     2401 cgagacgact cgctcctgat ggaactccac tccatagaac gcgaatcgcg ccacctcgtt
     2461 cttctgaagt gctacgggcg aattgattgt gggcagttcg ccacgctgga ttgaccagat
     2521 cacttcgcgc cggttcagtt cttgttcgag actgccgaca tcagcgtccg acaagcgcag
     2581 catctcgcga gtcatcgcca tgacgtggcg ttcctgcggc gtaatgattt ggtcggctga
     2641 tacttcggta atcaggtgtt gatatacgcg caccttgatg gtcgcgatgt tgcgcagccc
     2701 ggcagacgac gccctcacca gcgcatcgtc gatcaaatag ctcgatcgac gcgcttccga
     2761 acagcgcggc gaacgaaggc cgtcggcaga caacgcaaca gaaccagacc ggcgcgatga
     2821 catcgaccaa acctcgatca tctgcgatct tcgaggcttg ctcttcatcc agcgctgaaa
     2881 actgcaccgt cttcacgttg ccggcgatat cgtgcccctt caccttccac cgcatacggc
     2941 ccctgacagt tggcatccag gaatgctcac tggcgcgacg cgagtcactt ttgcgtatcc
     3001 attgctaact tcaggtcgcc gcccggatca tccagtacca tggttagcga ccagccacgc
     3061 cgccgtagct ttacttgatc ttgaggagaa gtgacgattg ctttcaatga agaatcgacc
     3121 catgttttag cttcaaggtc gttcttgaat ccgcctacgt tcaaggcgaa acgatcgagc
     3181 aattcccgat aatgaagcat cgaacgcgct gcatcggtgc cagtgccaag agccgtgccg
     3241 acggtctcag gatgtagcag cacgccgacc atccacggct tataaaccat gacggtaacg
     3301 tgcgcatatg tgatgtgatc gccgttagtt tcaaggcgaa tcgagcgaag gttgtcttcg
     3361 gaaaacccaa gttgcgttat cgttccatct acagaccgaa cttcttttgg atcgatgacg
     3421 aattgacttc cgatgccggc caacacttcg tcccgcgaga accgccgggg cttcaaagcc
     3481 aacatcgcag ccacgacgat tgtggcaatg cacaggactc cgacggcgaa aaatcgagtc
     3541 cgccgcgatt gcacaatacg tttcagccac gcatcttgcg ggtcgaccct ggacgcatac
     3601 ggcaccgtcg atggaccggt ttcgaccagc gggattcgtg cttccgccgt cgccgcatgg
     3661 gcgcctgtcg cgtcgggcag tcgcttaagc ttggaaacaa gcacgccgtc cgcgttggcc
     3721 agcgccaatg cagccttttc cgaatccgcc tcaaccgtga tcgaacgatc agcgccggaa
     3781 gatttgtccg cgccaagtgc ccgccatcgc atgtgacgcc cctttcactt gccccgtttg
     3841 tcgggcgatc gaggatgctt tttggtatcg agccgccact tcatttgaga tcgtcgcgcc
     3901 gttcgaactc accttgatcg ggacgctcaa gaccaaggcc ggcacatgag ccgtaggtct
     3961 cctcgacaaa gcaatgccgc tcggtttcgt ctcgcggtgc atctgctttg gtttcggcaa
     4021 tcggcgcgat catcaccaac acgcgctgag ccgcttcttc ggcgcccaat ggcacgcgcg
     4081 gcgttagaac gccatccgat ccgatctgtg actggatctg aattgctttc atcgctgcag
     4141 catctccgcg cgcccaatat gccaatttta cgccttcttc agcatattcc tcagcacggt
     4201 atgcaggatc ccgccgttac gataatactc aatctccacc ggcgtatcga tgcggcagat
     4261 ggtggtgaac tcgaccggtt cgccttcgcg gctggtcacg cgcacctgca cgtcctgtag
     4321 cgggctgatc tcgtcgctga tctgaatgtc gaacgtgccg acgccggtca ggccaaggct
     4381 gctgcggctt tggccacgct tgaattgcag cggcagcacg cccatgccga ccaggttgct
     4441 gcggtggatg cgctcgaagc tttcagcgat gacggcgttg acgccgagca gataaacgcc
     4501 cttggcggcc cagtcgcgcg atgagcccat gccgtagtcc ttgccagcca gcacgatcag
     4561 cggcacgccg tcggcttggt acttcatgct cgcgtcgtag atgctcatca cttcgccagt
     4621 cggcaaatac ttcgtcaccc cgccttcagt gccgggggcc agctggttgc gcaggcggat
     4681 gttcgcgaac gtgccgcggg tcattacgcg gtcgttcccg cggcgggaac cgtagctgtt
     4741 gaagtcgatc ggctgcacgc cgtgctccat cagatatctt ccggccgggc cgtccttctt
     4801 gatcgcgccc gcgggcgaga tgtggtcggt cgtcacgctg tcgcccacca tcaccagcac
     4861 gcgagcgccg atgatcggct tcaccttcgc cggctctttg cccaagttcg tgaagaacgg
     4921 cggctcctgg atgtaagtgc tgtgctcgtc ccagttgaac aattcgccgc ccttcaccgg
     4981 gatcttgttc cactcggggt tgccgttctc gacgttgccg tattgcttct ggaacatctc
     5041 gggcgagatg ctggcgacga tcgcatcgtg cacttcctgc tgcgtcggcc agatgtcgcg
     5101 caggtaaact ttttcgccct tggcgttctg ctggatcggc tcgtccatga tgttgagatc
     5161 catcgtgccg gccagcgcat atgcgacgac cagcggtggc gatgcgagat agtttgcgcg
     5221 cacgtgcgga ttgatgcggc cttcgaagtt tcgattgccg gacaagaccg agcaagcgac
     5281 gagatcgccc ttctcgatcg cattggcaat cggctcgggc agcgggcccg aattgccgat
     5341 gcacgtcatg cagccgtagc ccgtcgtctg gaagcccagc ttgtcgaggt attcgctgag
     5401 gcccgacttg ttgaagtaat ccgtgacgac gcgcgatccc ggcgacagcg acgtcttcac
     5461 gtacggcggc accttcagcc ccttttccac cgctttcttc gccaccaaac cggcggcgat
     5521 catcacgctg gggttgctcg tgttggtgca gctggtgatg gcggcgatga cgacgtcgcc
     5581 gtgcgtgagc ttgtgccact ggtcgtcatc catcgcgacg aacgccgcgt ccttctgcgt
     5641 cgagcctttc aggtgttcaa ccgtcgcggc ctccttttcg tcttccttcc ccgcggcgac
     5701 agccgtgacg tcggacgtgt cgtgctcctg cacctgcgcc gcctgggccc gaaacggcgc
     5761 caccggacct tcagcgctcc accgatccaa acgaggcgtc gcggactccc ctacttcctt
     5821 cttgaa
//
LOCUS       DATIMH010000557         7076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1581042, whole genome shotgun sequence.
ACCESSION   DATIMH010000557 DATIMH010000000
VERSION     DATIMH010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581042"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(417..1736)
                     /gene="hemL"
                     /locus_tag="VLI90_09665"
     CDS             complement(417..1736)
                     /gene="hemL"
                     /locus_tag="VLI90_09665"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HSV14517.1"
                     /translation="MADTVTQRSTINSGAAFQRAQQVLPGGVDSPVRAFKAVGGTPLF
                     IKEGEGCIIKDVDGNEYIDYVASYGPLIAGHANERVVAALSKAIGRGTSFGAPTELET
                     QLASTIVSALPAMEMVRFVNSGTEATMSAIRLARAATSRDLIIKCIGCYHGHVDGLLA
                     EAGSGALTLGTPSSPGIPKSTAATTVLVPYNDLAAAQAVFKKFEKQIAAFIVEPVAGN
                     MGVVPPVSGYLQGLRDLCTQNGSLLIFDEVMTGFRVAWHGAQGLYGVNPDLTCLGKII
                     GGGLPVGAYGGSRGLMEMISPAGPVYQAGTLSGNPLAMSGGVATLEILQEPEAYETLE
                     KRGATLAAGLAKAAKDAKVPVAINRVGSMIGLFFVKQSGQAVTNFAEATSGDTAAYAR
                     FFNAMLDNGVYLAPSMYEAIFVGLAHTDDVIEQTIDAAAKSFQAAAR"
     gene            complement(1988..2242)
                     /locus_tag="VLI90_09670"
     CDS             complement(1988..2242)
                     /locus_tag="VLI90_09670"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HSV14518.1"
                     /translation="MLAIKVVPGASRDRIAGEYGGALKVTVSQAPERGAANKAVIALI
                     AEALGVPTAQVEITRGHTSPRKEVVIRGISKDEILRRLGA"
     gene            complement(2259..3023)
                     /locus_tag="VLI90_09675"
     CDS             complement(2259..3023)
                     /locus_tag="VLI90_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSV14519.1"
                     /translation="MPTLEGRQSVLAALQARQRRFEVILVAHGAHVEKLQEMLDVAGE
                     MNVPVKQVERRELDAMSHGATHGGVVAIVTAKPRMSPAKLLEMVDRLSRPPLLLLLEG
                     VDDARNLGFTLRSAEALGVDAVLIKKHLWDFDETEVARPASGAYERLPLVQIDTIDPL
                     RELQRRNVRIYGCIAGAKRTIYERDLTRPVLLAIGGEKRGLSGAVREICDRFVTIPTV
                     GGASSLSLSHAGAIVMAEAHRQRMGKTQEANAASAD"
     gene            3120..4169
                     /locus_tag="VLI90_09680"
     CDS             3120..4169
                     /locus_tag="VLI90_09680"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HSV14520.1"
                     /translation="MTKAHGYAAQTKTSSLAPFNFERRDVGPRDVQIEILFCGVCHSD
                     IHQVRDEWGGSIYPMVPGHEIVGRVTKVGAQVKKFKPGDAAGVGCMVDSCRECESCRD
                     GEEQYCDRGECVLTYNSRYKDGTPSYGGYSNQIVVDEAFALRISPKQNLAAVAPLLCA
                     GITTYSPLKYWNVSKGQRVGVVGLGGLGHMALKLAHSFGAHVTQFTTSPGKEQDAIRL
                     GADEVVLTRDPESLKKLAGSFHFIIDTVSALHDINLYLSLLRRNGNLTLVGAPDKPMA
                     VGAFPLIMKRRSFSGSAIGGIRETQEMLDYCAEHNIVSDIELTPIQRINEAYERMLRN
                     DVKYRFVIDMASLRE"
     gene            4403..5167
                     /gene="recO"
                     /locus_tag="VLI90_09685"
     CDS             4403..5167
                     /gene="recO"
                     /locus_tag="VLI90_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HSV14521.1"
                     /translation="MPLVSDRCICLRKVEYSETSQILALFGRAHGLFRVIAKGAHRRT
                     KAGASKFDGGLDLLDTGEAVFTFDLARDLNTLTEWKLRDGHLELRRSLRGMYLGLYAA
                     ELTSTLFEEHDPHPELFDRLESTITDLATPKAEESFLAFELDLLRQTGHLAELGNCVE
                     CGSVLSDRDATYFSPNRGGVICRNCEGATPDRLSFDPRLLRLAQQILRLPRANGSTQR
                     LPRLTRHQTDPLNRLFAEHIEYTLGKRLRMPKLVTS"
     gene            complement(5182..5652)
                     /gene="ndk"
                     /locus_tag="VLI90_09690"
     CDS             complement(5182..5652)
                     /gene="ndk"
                     /locus_tag="VLI90_09690"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSV14522.1"
                     /translation="MERTLIIFKPDAVQRGLCGEILARFERKGLQVVGMKLTKIPQAT
                     AEKHYEPHKGKPFYAGLIQFMTSSPVVVLALAGKDAIAIARNLMGATFGSKAAPGTIR
                     GDFGVSNSFNLIHGSDSPESAQRELALFFKPDELQDWKPTIQSWVYDMSKGTPE"
     gene            complement(5811..6263)
                     /locus_tag="VLI90_09695"
     CDS             complement(5811..6263)
                     /locus_tag="VLI90_09695"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HSV14523.1"
                     /translation="MAEIRTLTQFSVFLVNKPGVLGQVTRALADEKVNVIAMTLVDSQ
                     EHGVLRLVAANPETARQVLAKLNLPMTETDVLCLDLSNRPGALADVAGVLGTNHVNIN
                     YAYCTSGAPGGRTTGIFKVADPAKAIRVMKTSGGKHKEPDLHRGTSRR"
     gene            complement(6281..6640)
                     /locus_tag="VLI90_09700"
     CDS             complement(6281..6640)
                     /locus_tag="VLI90_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSV14524.1"
                     /translation="MEDLPLTVQEIEKYTIIEFRTASLMDPIVLENIGQAIYKLIDEQ
                     DKRRIILDFEKVEYISSQAIGIVLTMNKKLSKLPHATLILCAVGPKLMELIKITRLDK
                     VLKIKPSQREALKVVPN"
     gene            6785..>7076
                     /locus_tag="VLI90_09705"
     CDS             6785..>7076
                     /locus_tag="VLI90_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14525.1"
                     /translation="MSQAPSREPQMNPAGASAAGAVQIQRQFVNFAYFKLDPAFRRLD
                     DHDKLQARSEFLKLFQQPRKGLMCLTYSTVGLRPDTDFLLWRISLTTDDFQSQ"
BASE COUNT         1271 a         2276 c         2197 g         1332 t
ORIGIN      
        1 gcttccgtgc cggtttgccc gtgttgaggg aaagtaatac cggatcctgc gccaaagtaa
       61 agcagttttt gcgcgatttg cccaaagcac cccctgcaaa cgtgttcgtt gaccttggtg
      121 acactcgcgc cgggtcatcg ccgatgccgt ggagtttgtg aaagtggcgt agtgctgctc
      181 gacggcagga agtaccgggt caattcccca acaattgcct tcaaggggaa ttgcatagct
      241 ttccccctct cccggtattc cgggagaggg ctggggtgag ggtgatttgg aattttgagt
      301 gccgctggtg ctcgaactca ccctcaccct aaccctctcc cggcgtaccg ggagagggga
      361 ccaggacgcg cggcagctat atatgcgagt gccctgcgac tgccgcactg cgcagatcat
      421 cttgccgcgg cctgaaaact cttcgccgcg gcgtcgatcg tttgttcgat cacatcgtcc
      481 gtgtgcgcca ggccgacgaa gatcgcttcg tacatgctcg gcgccaggta cacgccgttg
      541 tccagcatcg cgttgaagaa gcgggcgtac gcggcggtgt cgccgctggt ggcttcggcg
      601 aagttggtca ccgcttggcc ggattgtttg acgaagaaca ggccgatcat cgagccgacg
      661 cgattgatcg cgaccggcac cttcgcgtcc ttggcggctt tggccaagcc ggcagccagc
      721 gtcgcgccgc gcttctccag cgtctcgtac gcttcgggct cctgcaggat ttccagcgtc
      781 gcgacgccgc cgctcatcgc cagcggattg ccgctcagcg tgccggcctg atagaccggg
      841 ccggcggggc tgatcatctc catcagccct cggctgccgc cgtacgcgcc cacgggcagg
      901 ccgccgccga tgattttgcc caggcacgtc aggtcagggt tcacgccgta cagtccctgc
      961 gcgccgtgcc aggccacgcg gaagccggtc atcacttcgt cgaagatcag cagcgagccg
     1021 ttctgcgtac acaggtcgcg caggccttgc aggtatccgc tcacgggggg cacgacgccc
     1081 atgttgccgg cgaccggttc aacgatgaac gcggcgatct gcttctcgaa tttcttaaac
     1141 accgcctgcg ccgcagccag atcgttgtac ggaacgagca cggtggtcgc cgcggtggat
     1201 tttggaatgc cgggactgct cggtgtgccg agcgtcagcg cgccgctgcc ggcttcggcg
     1261 agcaaaccgt caacgtgccc gtggtagcaa ccgatgcact tgatgatcag atctcggctg
     1321 gtggccgcgc gcgccaggcg gattgcgctc atcgtcgcct cggtgccgct gttgacgaag
     1381 cgcaccatct ccatcgccgg cagcgccgaa acgatcgtcg atgccaattg cgtttccaat
     1441 tccgtcggcg cgccgaagct ggtgccgcgg ccgatcgcct tcgatagcgc cgcgacgacg
     1501 cgctcgttag cgtgacccgc gatcagcgga ccgtagctgg cgacgtaatc gatgtactcg
     1561 ttgccatcga cgtccttgat gatgcagcct tcgccttcct tgatgaacag cggcgtgccg
     1621 ccgacggcct tgaacgcgcg cacgggcgag tccactcccc cgggcagcac ttgctgcgcg
     1681 cgctggaacg cggcgccgga attgatcgtc gaacgttggg taactgtgtc agccatgaat
     1741 gcaatacttt ctgcggcgat ggtaagaagc ggcgtgacgc atcgcaacca tgcggcggcg
     1801 aagctgggcc aaggatagag atgtaattcg tgcgccaccg tgtgcgaaaa gacgggcgaa
     1861 agttcaaaat ccttagctga ttgagggaag tttaatagcc caggccggga ggccggggat
     1921 cgactgagaa aacgtgcaac atccgcagtc cgattcccgg ccttccggcc tgggctgtca
     1981 aatttcttca cgcgccgagc cgtcgcagga tctcgtcttt cgagatcccg cggatgacca
     2041 cctccttgcg ggggctcgtg tggccgcgcg tgatttccac ttgcgcggtg ggaacgccga
     2101 gcgcctcggc gatcagcgca atgaccgcct tgttggcggc cccgcgctcg ggggcttggc
     2161 tgacggtaac cttgagcgcg ccgccgtatt cccccgcgat tcgatcgcgc gaagcgccgg
     2221 ggacgacctt gatcgccagc acgacgccgc cgttacgttc agtcagctga agcggcattc
     2281 gcctcctgcg tctttcccat gcgctgtcgg tgcgcctcgg ccatcacgat cgcgccggcg
     2341 tgtgagaggg agagcgacga agcgccgccg acggtgggaa tggtgacgaa gcgatcgcag
     2401 atttcgcgga ccgcgccgga gagcccgcgc ttttcaccgc cgatcgccag cagcacggga
     2461 cgcgttagat cgcgctcgta gatcgtgcgc ttggcgccgg cgatgcagcc gtagatgcgg
     2521 acgttacggc gctgcagctc gcgcagcgga tcgatggtgt cgatctgcac gagcggcaat
     2581 cgctcatacg ccccgctggc gggacgggcg acttcggtct cgtcgaaatc ccacaggtgc
     2641 ttcttgatca gcaccgcgtc cacgccgagc gcttctgccg atcgcaatgt gaatcccagg
     2701 ttgcgggcat cgtccactcc ttcgagcaac agcagcagcg ggggcctact cagacgatcc
     2761 accatctcga gcagctttgc cggcgacatg cgcggctttg cggtgacgat cgcgacgacc
     2821 ccgccgtgcg tggcgccgtg cgacatcgca tccaactcgc gccgctcaac ctgcttcacc
     2881 ggcacgttca tctcaccggc gacgtccagc atctcctgca gtttctccac gtgcgcgcca
     2941 tgcgcaacga ggatcacttc aaaccgccgc tgacgagcct gcaacgcggc gagaacggat
     3001 tgtcggcctt cgagcgtggg catctgcctc gacagtataa agccactgcc cttctaccgc
     3061 gcccggcgcc tatgctgtgg aacgcattga ttcgaccccg gccccggaag gagacactca
     3121 tgaccaaagc ccacggctac gctgctcaaa ccaaaaccag ttccctcgcc cccttcaact
     3181 tcgaacgccg cgacgtcgga ccgcgcgacg tgcagatcga aatcctcttc tgcggcgtct
     3241 gtcactccga catccatcag gtccgcgacg aatggggcgg atcaatctac ccgatggtgc
     3301 cgggccacga gatcgtcggc cgggtgacga aggtcggcgc gcaggtgaag aaattcaaac
     3361 ccggcgatgc cgcgggcgtc ggctgcatgg tcgattcctg ccgcgagtgc gaaagctgcc
     3421 gcgacggcga agagcaatat tgcgaccgcg gcgaatgtgt gctgacctac aacagccgat
     3481 ataaggacgg cacgccctcc tatggcggat actcgaacca gatcgtcgtt gacgaagcgt
     3541 tcgcgctgcg catctcaccc aaacaaaacc tggcggcggt cgcgccgctg ctgtgcgccg
     3601 gcatcaccac ctattcgccg ctgaaatact ggaacgtcag caaaggccaa cgcgtcggcg
     3661 tggtgggctt gggcgggctc ggtcacatgg cgctgaagct ggcgcactcg ttcggcgcgc
     3721 acgtgacgca gttcaccacg tcacccggca aggagcagga cgcgattcgc ctcggcgccg
     3781 atgaagtcgt gctcacgcgc gatcccgaat cgctaaagaa gctggccggc agcttccact
     3841 tcatcatcga caccgtctcc gcgctgcacg acatcaacct gtacctgtcg ctgctgcgcc
     3901 gcaatggcaa cctgacgctc gtcggcgcgc cggacaaacc aatggccgtc ggcgcgttcc
     3961 cgctcatcat gaaacgccga tccttcagcg gctccgccat cggcggcatc cgcgaaacgc
     4021 aggagatgct cgactactgc gccgagcaca acatcgtctc cgacatcgaa ctcacgccga
     4081 ttcagcggat caacgaagcg tacgagcgga tgctgagaaa tgacgtgaag taccgcttcg
     4141 tgatcgacat ggcgtcgttg agggaatgaa gcgcaggcgc tggcccccac accgggttcg
     4201 gagcgtactc gcgacgaccc ggatcttctt cgcgcggcag aatctcgctt cgtcggagta
     4261 cctccgaacc cggggtagtg gggcaccttc aattctgcta ccaaaccagc acaagcgtct
     4321 ggccagaaca atcgggcatt tttggcgggg cacgagatca acaccaaacg cgggcgagat
     4381 gcccgtgcta caatcctgcc tcatgcccct cgtcagcgat cgatgcatct gcctgcgcaa
     4441 ggtcgagtac agcgagacct cgcagatcct cgcgctgttc ggccgggcgc acgggctctt
     4501 ccgcgtcatc gccaagggcg cgcaccgccg cactaaagcg ggggcgagca agttcgacgg
     4561 tgggctggac ctgctcgaca ccggcgaagc ggtcttcacg ttcgatctgg ccagggacct
     4621 gaacacgctc accgagtgga agctgcgcga tggtcacctg gaactgcgcc gcagcctgcg
     4681 cggcatgtac ctcggcctct atgcggcgga gctgacctcg acgctgttcg aggaacacga
     4741 tccgcacccg gaattgttcg atcgattgga atcgacaatc accgacctgg cgacgccgaa
     4801 ggcggaggaa tcatttttgg cgttcgagtt ggacctgctg cgccagacgg ggcacttggc
     4861 cgaactcggc aactgcgtgg aatgcggcag cgtcttgagc gaccgcgacg caacctattt
     4921 ctcccccaac cgcggtgggg tgatctgccg caactgcgaa ggggcaacgc ccgaccgcct
     4981 gagtttcgac ccgcggctgc tgcgcctcgc gcaacagatc ctgcgattgc cccgcgccaa
     5041 cggttcaacc cagcgcctgc cgcggttgac gcggcatcag accgatccgc tcaaccggtt
     5101 gttcgcggag cacatcgagt acacattggg caaacggctg cgcatgccga agctcgtgac
     5161 ttcctaacac cgcctcggcg ttcactccgg cgttcccttg ctcatgtcat agacccacga
     5221 ctgaatcgtc ggcttccagt cttgtagttc gtccggcttg aagaacagcg cgagttcgcg
     5281 ctgcgcgctc tcgggcgagt ccgatccgtg aatcaggttg aaggagttgc tgacaccgaa
     5341 gtcaccacgg atggtgccgg gggcggcttt ggaaccgaag gtcgcgccca tgagattgcg
     5401 ggcgatcgcg atcgcgtcct ttccggccag cgccagcacg acgacgggcg agctggtcat
     5461 gaactggatc agccccgcgt aaaacggctt acccttgtgc ggctcgtagt gtttctccgc
     5521 cgtcgcctgc gggattttcg taagcttcat gccgacgacc tgcaggccct tgcgttcgaa
     5581 gcgcgcgagg atttcgccgc acaggccgcg ctgaacggcg tcgggtttga agatgatgag
     5641 cgttcgttcc atggccgtgg ttcctccgga ttgatgacgt ctgagttata ggggaaacgc
     5701 gcgcacgcgt gaagcgaggg gacgcgcctc gcgtgcgaga gccgggccat cctcggcccg
     5761 aaccggcgcc ccgaacgggg gcggggacgc cccggctctc ctcggcaggg ttaccggcgc
     5821 gatgtgccgc ggtgcaggtc cggctctttg tgctttccgc cgctggtctt catcacgcgg
     5881 atggccttgg ccgggtcggc cactttgaag atgccggtcg ttcgcccgcc gggggcgccg
     5941 ctggtgcagt aggcatagtt gatgttgacg tgattggtcc cgagtacgcc ggccacgtcg
     6001 gccaaggcgc cgggacgatt ggatagatcg aggcaaagca cgtccgtctc ggtcatcggc
     6061 aggttcagtt tcgcgagcac ttggcgagcg gtttccggat tggcagcgac gagccgcagc
     6121 acgccgtgct cctgcgagtc cacgagcgtc atcgcgatga cgttcacctt ctcatcggcc
     6181 agcgcgcgcg tcacctgccc aaggacgcca ggcttgttga ccaggaaaac gctgaactgc
     6241 gtgagcgtgc ggatctccgc catggcgtgc ctccgggcgg ttagttgggt acgaccttca
     6301 acgcttcgcg ttgcgagggc ttgattttca acaccttgtc cagccgcgtg atcttgatca
     6361 gctccatcag cttcggcccg accgcgcaca ggatcagcgt cgcgtgcggc agcttgctta
     6421 gcttcttgtt catcgtcagc acgatgccga tcgcctggct ggagatgtat tctacttttt
     6481 caaaatccag gatgatccgg cgcttgtcct gttcgtcgat caacttgtag atggcctgac
     6541 cgatgttctc gagcacgatc gggtccatca gcgacgcggt gcggaattcg atgatggtgt
     6601 acttctcgat ttcctgaacg gtcagcggaa gatcttccat atcgtgcgcc ctgcgcgagg
     6661 tggcttcagc gaatcgcgtt tcccactgcg gcgtagcatc gcatgaagct gccagcgtcg
     6721 caagaatgaa acgcgcgcgg cgcctcaggg cgaaattgcc ggcgcgaaac catataatgc
     6781 ccgcatgtct caagctccat ccagagaacc tcagatgaat cccgccggcg cgagcgcggc
     6841 cggcgcggtg cagatccagc ggcagttcgt caacttcgcg tacttcaagc tggaccccgc
     6901 gttccgccgg ctcgatgacc acgacaagct gcaggcccgc agcgaattct tgaaactgtt
     6961 ccagcagccg cgcaaggggc tgatgtgttt gacctacagc acggtcggac ttcggcccga
     7021 cacggatttc ctgctctggc gcatcagttt aacgacagac gatttccaat cgcaga
//
LOCUS       DATIMH010000558         1713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5047350, whole genome shotgun sequence.
ACCESSION   DATIMH010000558 DATIMH010000000
VERSION     DATIMH010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5047350"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1482)
                     /locus_tag="VLI90_09710"
     CDS             complement(256..1482)
                     /locus_tag="VLI90_09710"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV14526.1"
                     /translation="MSGRAAFQYANFRYYMTARIFVTLASEMQAVAVGWQIYALTHRA
                     LDLGLVGLAQFLPGFVLFLAAGQTADRYPRQRILQCCYVAFAGISALLLGLSLRGLTT
                     VWPVYAALLLNGVVRCFNGPASQAFLPLVVPEEVFPNAVALGSGVFQGAMIAGPMIGG
                     LVYGISGSAAPVYAGSVVACLCAAVMMGRIQIAKVQRPRGAVNPGVLLEGLRYLWRAK
                     LTLGAMSLDMFAVLLGGAVALLPVYARDILKVGASGLGILRGAPGTGAVIMALVLAHW
                     PLRRHAGAIMLWCVAGFGAMTVVFGLSRNLLLSVAALVLMGACDMVSVVVRHTLVQLS
                     TPDEMRGRVSAVNSIFIGASNEVGQFESGITAAWLGAVPAVVIGGLGTILIVGLWSRI
                     FPELRRVNELQPTRET"
     gene            complement(1506..>1713)
                     /locus_tag="VLI90_09715"
     CDS             complement(1506..>1713)
                     /locus_tag="VLI90_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14527.1"
                     /translation="TGFEIAGADGSYVPADVKVDGNTLLVTSAQVASPATVRYAWADD
                     PVCNLVNQVGLPASPFRSDQPFYQ"
BASE COUNT          350 a          566 c          504 g          293 t
ORIGIN      
        1 cgtgtcgaca ttgttgatct gcacggtcgc gcccgaaatc gccgcgcccg ccggatccgt
       61 gatcgtccct gtgatctccc cgttcatggc cgcggcctgt ccgaagatat tgcctgcgag
      121 cgccattccg agggctagaa ccaccgcact gaacttcaac tgataactca taattgtctc
      181 tttctctgtg tgtgctctta gagtcctgag cttgattcca gatgcttcta tttgacgaag
      241 tatgccctat agatgtcacg tctctcgcgt aggttgcagc tcattaactc tacgtaattc
      301 gggaaaaatg cggctccaca atcccacaat cagaatcgtt ccgaggccgc caatgactac
      361 tgcggggacc gctcccaacc acgctgccgt gatccctgat tcgaactggc cgacttcgtt
      421 cgaggctccg atgaagatcg aatttaccgc gctgacgcgg ccgcgcattt catcgggcgt
      481 ggaaagctgc accagcgtgt ggcggacgac gacgctcacc atgtcgcacg cgcccatgag
      541 taccagagcc gccacggaga gcaggagatt ccgtgagagt ccgaagacca ctgtcattgc
      601 gccaaagccc gccacgcacc acagcatgat tgcgccggcg tggcggcgca agggccagtg
      661 cgcgagcacc agcgccatga tgaccgctcc cgtcccggga gcgccgcgca ggattcccaa
      721 cccactcgcg ccgaccttca gaatatcgcg cgcatacacg ggcagcagcg cgaccgcgcc
      781 gcccaataag acggcaaaca tatccagcga catcgcgccg agcgtgagtt tggcgcgcca
      841 taaatatcgc aggccctcga gcagcacgcc gggattgacg gcccctctgg gtcgctgcac
      901 tttcgcgatt tgtatgcgcc ccatcatgac tgccgcacaa aggcaggcca cgaccgatcc
      961 ggcgtaaacc ggcgccgcgc tgcccgaaat tccgtagact aaaccgccga tcatcggacc
     1021 cgcgatcatg gcgccctgga acactcccga gcccaatgcg acagcgttcg ggaacacttc
     1081 ctcgggcacc actagcggca gaaaggcctg cgatgcggga ccgttgaagc agcgaacgac
     1141 gccgttcaac agcagcgcgg cgtagacagg ccacacggta gtgagccctc gcagtgataa
     1201 tcccaacagc agcgcggata ttcccgcgaa agccacgtag cagcactgga ggatgcgctg
     1261 ccgcggatag cggtcggcgg tctgcccggc ggcgaggaag agcacgaagc caggcaggaa
     1321 ctgcgccaga ccgacgagtc ccagatcgag cgcgcgatgg gtcagcgcgt agatctgcca
     1381 gcccaccgcg accgcctgca tttcgctggc gagcgtgacg aagatccgcg cggtcatgta
     1441 atagcggaag ttggcgtact ggaacgccgc gcgtccgctc atgttctatg gttccgcaaa
     1501 tacgcctact ggtagaacgg ctggtcgctg cggaacgggc tcgccggcag gcccacctgg
     1561 ttcaccaggt tgcacaccgg gtcgtcggcc cacgcatagc gcaccgtcgc gggactggcg
     1621 acctgcgcgc tggtcaccag cagcgtgttg ccgtccacct tgacgtcggc cggaacgtag
     1681 cttccatcgg cgccggcgat ttcaaagccg gtg
//
LOCUS       DATIMH010000559         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3310753, whole genome shotgun sequence.
ACCESSION   DATIMH010000559 DATIMH010000000
VERSION     DATIMH010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3310753"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VLI90_09720"
     CDS             <1..1233
                     /locus_tag="VLI90_09720"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSV14528.1"
                     /translation="HNGGAIHFGPDGKLYIGVGENGNGNNAQSLTTDLGKMLRINADG
                     SIPTDNPFYGNTTGNNRAIWALGLRNPFTFAFQPASGRMFIDDVGQDTWEEIDDGIAG
                     SNYGWPVTEGPTTDPRFRGPLFAYPHGPNDSTGVAITGGTFCDPPVSPFGAVFNGTYF
                     FADLGVGFIHNFNPATNAETPFATGYQNPVDLKVDAQGNLFVLTIGDGAVHRISATLT
                     TLPTPTIVRPLAAQHFVAGRRFGFIGVATDAQDGRLKPSALSWDVQLWRDGVEQRSVK
                     QIAGRAIGFYDVPRVGEETGGSADVFYRIVLTGTNSRGQSATTVHDITPLTGTIHLLS
                     RVGPTRADGLQIAFDGQPEPTPFDFTGVVGSIHTIDAISPQTFDDETIIPLRFPNHRL
                     VIVRLGQATFVAPFRAAK"
     gene            complement(1302..3056)
                     /gene="ilvB"
                     /locus_tag="VLI90_09725"
     CDS             complement(1302..3056)
                     /gene="ilvB"
                     /locus_tag="VLI90_09725"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HSV14529.1"
                     /translation="MTTLNDTQTTAPATTATDKPRYKGKPGAQILHEMLVGPHKVDTM
                     FGYPGGAILPMFDQLYKTPAKFILNRHEQASGHCADGYARATGKVGVCIVTSGPGATN
                     TVTPLATAQLDSVPMVIFSGQVATKFIGNDAFQEADVTGITRPCTKWNYLIKDVRELP
                     RVVNEAFLVASSGRPGPVLIDLPKDVQVQVCTDEVDDTPREHILKRRKTSISGGSQKA
                     VMDAVELINKSEKPVLYVGGGAIASGASPIVRKLAEKGNIPCTTTLLGLGAFDETSPL
                     SMHMLGMHGSAYANYAVQNCDCLIAVGARFDDRVTGNLASFAPHAKIIHIDIDPSSIS
                     KVVDVDVPVAGDAKTSLEAMFPHIEYRERKDWHATIQAWKAKYPFHYLDDSKNAKPQY
                     VLEEINRQTQGDAIFTTGVGQHQMWAAQYIRWRYPRQMITSGGLGTMGYGLPSAMGAQ
                     LGSPGKTVIDIDGDGSYLMTCYELATIAEYNIPVKVCILNNDFQGMIKQWQDLFYEKR
                     YSNSPMKNPHFAKMAESFGVRGIRCEHKADVEKTVKEMLAHKGPVVVDFLVEPNEHVY
                     PMVPSGKGLHEMELGTLA"
BASE COUNT          580 a         1074 c         1079 g          673 t
ORIGIN      
        1 cacaacggcg gcgcgatcca cttcggtccc gacgggaagc tctacatcgg cgttggtgaa
       61 aacggcaacg ggaacaacgc gcagtcgctg acgacggact tgggcaagat gctgcgcatc
      121 aacgccgacg gatccattcc caccgacaat cccttctatg gcaataccac tggaaacaac
      181 cgcgcgatct gggcactcgg attgcggaat ccattcacgt tcgcgtttca gccagccagc
      241 gggcggatgt tcatcgatga cgtcgggcag gacacgtggg aggagatcga cgacggcatc
      301 gccggcagca actacggctg gccggtgacc gaggggccga cgaccgaccc gcggtttcgc
      361 gggccgctgt ttgcgtatcc acatggcccg aatgattcga ccggcgtcgc gatcaccggc
      421 gggacatttt gcgatccacc ggtctcgccg ttcggcgcgg ttttcaacgg cacgtacttc
      481 ttcgcggacc tcggcgtcgg tttcatccac aacttcaacc cggcgacgaa cgccgagacg
      541 ccgttcgcga cggggtatca gaacccggtg gacttgaagg tggacgctca gggaaacctg
      601 ttcgtcctga cgatcggcga cggggcggtg catcgcatct ccgctacatt gacgacgctg
      661 ccgacgccga cgatcgtccg gccgctggcc gcccagcatt tcgtcgctgg ccggcgcttc
      721 gggttcattg gcgtggcgac cgatgcgcag gatggccggc tcaagccgag cgcgctgtcg
      781 tgggacgtgc agctctggcg cgacggcgtc gagcagcgca gcgtgaagca gatcgccggt
      841 cgcgcgatcg gcttttacga tgtcccccgc gtcggcgagg agaccggcgg atccgccgac
      901 gtcttctatc gcatcgtgct gaccgggacc aactcgcgcg gtcagtccgc cacgacggtg
      961 catgacatca cgccgttaac cgggacaatt cacctgctgt cgcgcgtcgg ccccacgcgc
     1021 gccgacggct tacagatcgc gttcgacggc cagccggagc caacgccgtt tgatttcacc
     1081 ggcgtcgtcg gatcgatcca tacgatcgac gcaatctcgc cccagacgtt cgatgatgaa
     1141 acgatcatcc cgctacggtt ccccaatcat cgcctggtga tcgtgagact gggccaggcg
     1201 acgttcgttg caccgtttcg cgcagcgaag tagcgcactc gccggtgtcg aaggaattag
     1261 ttggatcgtc ggcgcatcgt ctaggatgac gaaagccaat cttacgccag cgtccccagc
     1321 tccatctcgt gcagtccctt gccgctgggc accatcgggt acacgtgctc gttcggctcg
     1381 acgaggaaat ccaccaccac cgggcccttg tgggccagca tttccttgac cgtcttttcg
     1441 acgtcggcct tgtgctcgca gcggatcccg cggacgccga acgattccgc catcttcgcg
     1501 aagtgcgggt tcttcatcgg gctgttgctg tagcgcttct cgtagaacag gtcctgccac
     1561 tgcttgatca tgccctggaa gtcattgttc aggatgcaga ccttcacggg gatgttgtat
     1621 tcggcaatcg tcgccagctc gtagcaggtc atcaggtagc tgccgtcgcc gtcgatgtcg
     1681 atgaccgttt tgccggggct gccgagctgc gcgcccatgg cgctgggcag gccgtaaccc
     1741 atcgtgccca gtccgccgct ggtgatcatt tggcgggggt aacgccagcg gatgtactgc
     1801 gccgcccaca tctggtgctg gcccacgccg gtggtgaaga tcgcgtcgcc ctgtgtctgg
     1861 cggttgatct cttccagcac atactgcggc ttggcgttct tgctgtcgtc caggtaatgg
     1921 aacggatact tcgccttcca cgcctggatc gtcgcgtgcc aatccttgcg ctcgcgatat
     1981 tcaatgtgcg ggaacatcgc ctcgagcgac gtcttggcat cgccggcgac ggggacatcg
     2041 acgtcgacca ccttgctgat cgacgacgga tcgatgtcga tgtggatgat cttcgcgtgc
     2101 ggcgcgaagc tggcgagatt gccggtcacg cgatcgtcga agcgcgcgcc gacggcgatc
     2161 aggcaatcgc aattctgcac cgcgtaattc gcatacgcgc tgccatgcat gccgagcatg
     2221 tgcatgctca gcgggctggt ttcgtcgaac gcacccaagc ccagcagggt agtcgtacag
     2281 gggatgttgc ccttctcggc cagcttgcgg acgatcggcg atgccccgct ggcgatcgcg
     2341 ccaccgccga cgtacagcac gggcttctcg ctcttgttga tcagctcgac cgcgtccatc
     2401 acggccttct ggctgccacc ggagatgctg gtcttgcggc gcttgagaat gtgctcccgc
     2461 ggcgtgtcgt ccacttcatc ggtgcacacc tgcacctgca cgtccttcgg caaatcgatc
     2521 agcaccggcc cgggccggcc gctcgatgcg acgaggaatg cttcattcac gacgcgcggc
     2581 aactcgcgca cgtctttgat gagatagttc cacttcgtac atggccgggt gatgccggtg
     2641 acgtccgctt cctgaaatgc gtcattgccg atgaacttcg tcgccacctg accgctgaag
     2701 atgaccatcg gcacgctgtc cagctgcgcg gtggccagcg gcgtcacggt gttcgtcgcg
     2761 cccggacccg acgtcacgat gcacacaccc accttgccgg tcgcgcgggc gtagccgtcg
     2821 gcgcagtgac ccgacgcctg ctcgtggcgg ttcaggatga acttggctgg cgtcttgtag
     2881 agttggtcga acatcggcag gatcgcgccg ccggggtaac cgaacatcgt gtcgaccttg
     2941 tgcgggccga ccagcatttc gtgaaggatc tgagcgcccg gcttaccctt gtatctgggc
     3001 ttgtccgtgg cggtggtcgc gggggcggtg gtttgggtgt cgttcagggt ggtcatggtc
     3061 aactcgtttt aggttaggcg gcctgcaagg cgtcgcgaaa atcaagccac gcactgtacc
     3121 gggcgatggc cgtatcttca agaattatca gtgattcggg gcgaaaacag gcggggcggg
     3181 ccgggtaggc aaaggtcgcg ggcagcaagc ctaccggcct aaggtactac caccgtgggg
     3241 gtggtaaggc gaagcgcaag atctggcccg agattcatga ggtacagcgt acgaacgcgg
     3301 cgagccatcg tcaagtccgc ggcagctcgc ggcagcatgg tttcccagcg agggtgtaac
     3361 tcctaattgt ggcgagtgtc ccgccggtcc cctctcccgc gtacgc
//
LOCUS       DATIMH010000560         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1581442, whole genome shotgun sequence.
ACCESSION   DATIMH010000560 DATIMH010000000
VERSION     DATIMH010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581442"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="VLI90_09730"
     CDS             complement(<1..1573)
                     /locus_tag="VLI90_09730"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HSV14530.1"
                     /translation="MPETPLQRPVMIYDGQCFFCQRWVERWRRNIGHVLDFEPYQQAA
                     GRFPQIDPTAFGQAVHLVEPDGRVSRGAEAVLRAHALAGRRRGWWWAYRYLPGFSAVA
                     EQVYSTIAAHREAADQIDLQLLGRRTDIGTHRLTRQVFLRSLGVIYVIAFGSLWVQIN
                     GLIGSGGILPIDHYLQLVNQRISGSDAIRLLPTLCWFNSSDSFLQTLCGGGVAAGLAV
                     IAGIAQLPALVVAFVCYLSLTVAGQVFLGFQWDALLLEAGFLSIFFAPWEFWSNRSAD
                     NPRISGAPSSIILWLLRWLLFRVMFTSGIVKLTSADVAWRGWTAMQYHYETQPLPPWT
                     AWYFFQLPAWFQKASCGVVFFAELIVPLLIFGPRRVRLVAFWLIVLFQCLIIATGNYG
                     FFNLLTIALCFTLPDDSFWRWLVRRPEPPIEQRRASRWRWPVWITAPIAAVLLAVTIP
                     VASAAFERPVRWPRPILALYEWIAPLRIANSYGLFAIMTTERPEIIIEGSDDGVLWKA
                     YAFKWKPGDVTRRPAF"
     gene            complement(1711..2346)
                     /locus_tag="VLI90_09735"
     CDS             complement(1711..2346)
                     /locus_tag="VLI90_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14531.1"
                     /translation="MRYLIRVIPVVLLAVACRVVRADASPATQSSISQPPTTQLGDLS
                     TPRAMMLTYDAQSGAGPEAFLPFFLTHNADEERLVRAEARTDAMLGMLQVIVEKKWGA
                     AAVDTVLHAFDSKTRDDIKSAKVSVTGDVALMMWPDKAPPLQIVRTPEGWKIDAAAMT
                     KSIGETVDEYVKDLQSIAPVVTDIAGAIADRKLATPEAAAAEARRRLAALH"
     gene            complement(2532..3596)
                     /locus_tag="VLI90_09740"
     CDS             complement(2532..3596)
                     /locus_tag="VLI90_09740"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HSV14532.1"
                     /translation="MPAESAQIRDLLGNDADSLLTYKAKGFDKSQLHLPGPDFIDRVV
                     SASDRRPAVLRNFQQILNHGRLGGTGYVSILPVDQGIEHSAGASFAPEPIYFDPESIV
                     KLAIEGGCNAVASTLGVLGSVARKYAHKIPFLVKLNHNEFLSYPNKYDQIQFGSVRQA
                     IDMGAVAVGATIYFGSAESDRQIQEVSKMFAEAHEHGLVTVLWCYLRNNAFKTADKDY
                     HVAADLTGQANHLGVTIQADIIKQKLPETNGGFTALKFGKTDQRVYSDLTPGDHPIEM
                     TRYQLANCYMGRSPLINSGGASGAHDFADAVRTAVINKRAGGMGLISGRKAFQRPMAE
                     GVKLLNLIQDVYLDASITVA"
     gene            complement(3675..>4130)
                     /locus_tag="VLI90_09745"
     CDS             complement(3675..>4130)
                     /locus_tag="VLI90_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HSV14533.1"
                     /translation="QMSAMFARFPVDAFEVVVHQHMPMFHMEHCVFAHTLSSGKDFRD
                     CGRPCERHQVDLRDRAGEPHPLIPDVGCRNTVFNAKAQSAAEFVPEMLKLGLRHFRVE
                     LLRESPAEVGLLLDRYAGVITGRADARTTVRSLRVLNQLGVTRGTLDRE"
BASE COUNT          801 a         1415 c         1184 g          730 t
ORIGIN      
        1 aaaacgccgg ccgccgcgtc acgtcgcccg gcttccactt gaacgcgtac gccttccaca
       61 gcacaccatc gtcgctgcct tcgatgatga tctccggccg ttcggtcgtc atgatcgcga
      121 acagcccgta actgttggcg atccgcagcg gcgcgatcca ctcgtacagc gcgaggatcg
      181 gccgcggcca gcggacaggc ctttcgaacg cggccgacgc gaccggaatg gtcaccgcca
      241 gcaagaccgc ggcgatcggc gccgtgatcc acaccggcca tcgccagcgc gatgctcgtc
      301 gctgctcgat cggtggctcc ggccgtcgaa cgagccatcg ccagaatgaa tcatccggca
      361 acgtgaaaca gagcgcgatc gtcagcaggt tgaagaaccc atagttgccc gtcgcgatga
      421 tcaggcattg gaacagcacg atgagccaga acgcgaccag tcgcacgcgg cgtgggccga
      481 agatcagcag cgggacgatg agttcggcga agaagacgac gccgcacgat gccttttgaa
      541 accacgccgg cagctggaag aaataccacg ccgtccacgg cggcagcggt tgggtttcgt
      601 agtgatactg catcgcggtc cacccgcgcc acgccacgtc ggcgctggtc agcttgacga
      661 tgcccgacgt gaacatcacg cggaagagca accatcggag cagccagagg atgatcgatg
      721 atggcgcgcc gctgatgcgc gggttgtcgg cgctgcggtt gctccaaaac tcccacggcg
      781 caaaaaagat ggatagaaat ccagcttcaa gaagcagcgc atcccactga aatccgagga
      841 acacctgccc cgccaccgtc aacgacagat agcacacgaa tgcgacgacc agcgccggca
      901 gttgtgcgat tccagcaatc acagctaacc ccgccgccac cccgccgccg cagagcgttt
      961 gcaggaatga gtcgctggag ttgaaccagc acagcgtggg cagcaatcgg atcgcatcgc
     1021 tgccgctgat gcgctggttg accagttgga gatagtgatc gatcggcaag atgccgccgc
     1081 tgccgatcag gccgttgatc tgcacccaca gcgacccgaa cgcgatgacg tagatgaccc
     1141 cgagcgatcg caggaatacc tgccgcgtca accggtgtgt cccgatgtcg gtgcgccgcc
     1201 ccaacagttg caggtcaatt tgatccgccg cctcgcgatg cgcagcgatc gtcgagtaga
     1261 cctgctccgc caccgccgaa aaccccggca ggtatcgata cgcccaccac cacccgcgcc
     1321 gccggccggc caatgcgtgc gcccgcagca ccgcctccgc gccgcggctc acgcgcccgt
     1381 ccggctccac cagatgcacc gcctgcccaa acgcggtggg atcgatctgc ggaaatcgcc
     1441 ccgccgcctg ctggtatggc tcgaagtcaa gaacgtgccc gatattccgc cgccaccgct
     1501 ccacccaccg ctggcagaag aaacactgcc cgtcataaat catgaccggc cgctgaagag
     1561 gcgtctcagg catgatgccg ttgtactgcg cgcgagtgta aatgcggaag atgcgggagc
     1621 gccgcttggt ttgtagctgc gacgcctgcg tcgcctctta gagccggagg cggcgcaggc
     1681 ggcgcagcta ccggaatcga atcccgccga tcagtgcagc gccgctaacc gccgccgcgc
     1741 ttccgccgcg gccgcttccg gcgtcgcgag ctttcggtcg gcgatcgcgc cggcgatatc
     1801 cgtcaccacc ggcgcgatcg actgcaggtc tttaacgtac tcatccaccg tctcgccgat
     1861 cgacttcgtc atcgccgccg catcgatctt ccacccctcg ggcgtccgaa cgatctgcag
     1921 cggcggggcc ttatccggcc acatcatcag cgcgacgtca cccgtcacgc tcacctttgc
     1981 cgatttaatg tcgtcccgcg tcttggaatc gaacgcgtgc agcaccgtgt ccaccgccgc
     2041 cgcgccccac tttttctcga ctatcacctg cagcatcccc agcatcgcat ccgtccgagc
     2101 ctccgcccgc accagtcgct cctcatccgc gttgtgcgtc aagaagaacg gcaaaaacgc
     2161 ctccgggccc gcgcccgatt gcgcatcgta cgtcaacatc atcgcccgcg gcgtgctcag
     2221 atcccccaac tgcgtcgtcg gtggctgcga tatgctggac tgcgttgcag gtgacgcatc
     2281 cgcccgcacg acgcgacatg cgacggcgag aagaaccacg gggatcacac ggatgagata
     2341 gcgcatgact gcatcatatc cgggattcaa gtcgcgcgca ggtttatcga tccgctgagc
     2401 gtatttgcgg aattacgaac gcgcacgagg cgctcgcccg gactaaccgc ttcagcggtt
     2461 agtgcattca ttcgccattc ggatttcatt cggggattcg gacttcgcca ttctgatttt
     2521 ccccccgccc ctcacgccac cgtgatcgac gcatccagat atacgtcctg aatcagattc
     2581 agcagcttca cgccctctgc catcgggcgc tgaaacgcct tgcgaccgct gatcaaaccc
     2641 atccctccgg cgcgcttgtt aatgacagcc gtgcgcacgg cgtcggcgaa atcgtgcgcc
     2701 ccgctcgccc cgccgctgtt gatcagcggg ctccggccca tgtagcaatt cgccagctga
     2761 taccgcgtca tctcgatcgg gtgatcgccc ggcgtcaggt cgctgtagac gcgctggtca
     2821 gttttgccga acttcagcgc ggtgaatccg ccgttcgtct ccggcagctt ctgcttgatg
     2881 atgtccgcct ggatcgtcac gcccagatga ttcgcctgcc ccgtgagatc ggcggcgacg
     2941 tgataatctt tatccgccgt cttgaacgcg ttgttccgca gataacacca cagcaccgtg
     3001 accagcccgt gctcgtgcgc ctcggcgaac atttttgaca cttcctggat ctgccgatcc
     3061 gactcggccg atccgaaata aatcgtcgcg cccacggcca ctgcgcccat gtcgatcgcc
     3121 tgccgcacgc tgccgaactg gatctgatcg tacttgttcg gatagctcag gaattcattg
     3181 tgattcagct tcaccaggaa cgggatcttg tgcgcgtact ttcgcgccac cgagccgagc
     3241 acgcccagcg tcgatgccac cgcgttgcac ccgccttcaa tcgcgagctt cacgatgctc
     3301 tccggatcga agtaaatcgg ctccggggca aagctcgcgc ccgcagagtg ttcgatgcct
     3361 tgatcgaccg gtaggatcga gacatagccg gtgccgccga gccggccgtg gttcagaatc
     3421 tgctgaaagt tccgcagcac cgccgggcgg cggtcgctcg cgctaacgac gcggtcgatg
     3481 aagtccggcc cgggcagatg cagctgggat ttgtcgaacc ccttcgcttt ataggtgagg
     3541 aggctatcag cgtcattgcc gagaagatca cggatctgag cagattcggc aggcatggtt
     3601 ctccttcttc ttcgtagcac gggctaccgg cccgtgccga tcgtctgaac gcgtgagctg
     3661 gtagcccatg ctacttattc ccgatccaat gtaccgcgcg tcacaccgag ttggttcaaa
     3721 acgcgcagcg accgcacggt cgtccgcgca tcggcgcgtc cggtgatgac gccggcgtaa
     3781 cgatcgagca acaggccgac ttctgccggc gactcgcgca gcagctcgac acgaaagtgc
     3841 cgcaggccca gcttcagcat ctcaggcaca aactccgccg ccgactgcgc cttggcattg
     3901 aagacggtgt tgcgacaacc gacatccgga atcagcgggt gcggctcacc cgcgcggtcg
     3961 cgcaagtcca cctgatgccg ctcacaaggc cggccgcagt cacgaaaatc cttgccgctc
     4021 gatagcgtgt gcgcgaaaac gcaatgctcc atgtggaaca tcggcatgtg ctggtgcacc
     4081 acgacctcaa atgcatccac cggaaaccgc gcgaacatcg cagacatctg
//
LOCUS       DATIMH010000561         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1294597, whole genome shotgun sequence.
ACCESSION   DATIMH010000561 DATIMH010000000
VERSION     DATIMH010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294597"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..956
                     /locus_tag="VLI90_09750"
     CDS             87..956
                     /locus_tag="VLI90_09750"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HSV14534.1"
                     /translation="MSDSPVHHDTIASGDHRLHRITAAPDGIPSARLAILHGYGDHAG
                     RFVHFMRWMAERDVACYAIDQRGHGESTGRRCHINNWSEYLDDLATFMATPELATKTD
                     QPPLFVLGHSHGGLVVAAAGIRQQLDRCRGVILSSPYLDLKMPVPWHKRWIGRIGARL
                     FPAMPVRSGVADQPLSRDPQMNLDTQSDPHCLGIATPRWFETATAAQAEVRAKAAEFQ
                     LPLLMLVATADTIANPTAAVAFYEHCGSSDRTLKVYEGFRHELLRELGREQVFEDVLD
                     FVRARANTSAVLS"
     gene            complement(1179..>1584)
                     /locus_tag="VLI90_09755"
     CDS             complement(1179..>1584)
                     /locus_tag="VLI90_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14535.1"
                     /translation="YFVRNAGDNTQYLSSADIPSPLLGLTWRPAYEAFFEDATGTLQS
                     LVGDDQVVFTPEPSTDWIGWLEGTYPVPTSVGTVASDAAESLRGSVTTAAGMFAYGVV
                     NTDPVTVKMVAGDTFLPVLKVPKAVFIATVKF"
BASE COUNT          289 a          555 c          475 g          265 t
ORIGIN      
        1 gcgtcctcgg cgtctcggcg gttgcattcg catccgaatc acaaaacccg ttgcgtcgcg
       61 atccgcttcc gccacaatca cgatcaatgt ccgattcccc ggtccaccac gacaccatcg
      121 ccagcggcga tcatcggttg caccgcatca ctgccgcgcc cgacggcatt ccatccgctc
      181 gccttgcgat cctgcacgga tatggcgacc acgcggggcg ctttgtgcac ttcatgcgct
      241 ggatggccga acgcgacgtc gcctgctacg cgatcgacca gcgcggccac ggtgaatcga
      301 ccggccggcg gtgccatatc aacaactggt cggaatacct ggacgacctg gcaacgttta
      361 tggcaacgcc agaactggca accaaaaccg atcaaccgcc gctattcgtg ctcggacaca
      421 gccacggcgg gctggtcgtc gcagccgcgg ggattcggca gcagctggac cgttgccgcg
      481 gcgtgatcct gtcgtcgccg tacctggatt tgaagatgcc ggtgccgtgg cacaagcggt
      541 ggatcggccg gatcggcgcc cggctattcc cggcgatgcc ggtccgcagc ggcgttgccg
      601 atcaaccgct gagccgcgac ccgcagatga acctcgacac gcagtccgac ccgcactgcc
      661 tgggcatcgc gacaccgcga tggttcgaga ccgccacggc agcgcaagcc gaagtccgcg
      721 ccaaagccgc cgagttccag ctcccgctgt tgatgctggt cgccacggca gacacgatcg
      781 ccaaccccac cgcggcggtg gcgttttacg aacactgcgg atcgagcgat cgaacgttga
      841 aggtgtatga aggctttcga cacgagctgc ttcgtgaatt gggccgcgaa caagtcttcg
      901 aagatgtgct cgacttcgtc cgcgcccgcg cgaacaccag cgccgtttta tcctgagcgc
      961 aggcgtactc gccggagtcg aaggatctag cccagaagtc gatgaacgcc ggctcgccgg
     1021 acgatcaccg gcagtcgctt gcggcttcgc agaccaggcg gcgctgatct gcgaaaccgc
     1081 aagcgactgc cgcgcccttc gcgcgactgg cagttcaccc actcccagcc agatccttcg
     1141 actccggcgg cgtaccgcct gcgctcagga tgaacttcct agaacttcac cgtcgcaatg
     1201 aacaccgcct tcggcacctt aagcaccggc aggaacgtgt cccccgcgac catcttcacc
     1261 gtcaccgggt ccgtgttcac cacgccgtac gcgaacatcc ccgccgcggt tgtcacgctg
     1321 ccgcgcagcg attcggccgc atccgacgcg acggtgccca cgctcgtcgg caccgggtac
     1381 gtgccttcga gccagccgat ccaatcagtg ctgggctccg gggtgaagac cacctggtcg
     1441 tcgccgacca gcgactgcaa tgtgccggtc gcgtcttcga aaaacgcttc gtacgccggc
     1501 cgccaggtga ggccgagcaa cggcgagggg atgtcggcgc tcgacaggta ctgcgtgttg
     1561 tcgccggcgt tgcgcacgaa ataa
//
LOCUS       DATIMH010000562         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3886987, whole genome shotgun sequence.
ACCESSION   DATIMH010000562 DATIMH010000000
VERSION     DATIMH010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3886987"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VLI90_09760"
     CDS             <1..1014
                     /locus_tag="VLI90_09760"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSV14536.1"
                     /translation="QVHDLAIAMMHTTGGGALLVVAIFGLIVGIGFKISAVPFHFWCP
                     DVFEGASIDVTTFLSVASKGAGLILLLRIVEMLGAASDFTANGTLTAIAAVIGTIGAI
                     TATVGNTGAFVQKNIKRLLAYSSIAHAGYMLCAVSLMVKSQSAQRGYDPASSAAQAIL
                     LYLAIYALMNLGAFTVAALVARQSNGGETLADFTALGRRSPLLAACMFCCLISLIGLP
                     PFAGFTAKWNLLWVLFQNGGWWWALIIVIGVNTIISAFYYFRVIRAMYLEDNEAPVFH
                     GNPLGVAISVACSVALVLLLLGFSPLSRMTNNYSQLYLSGPTSPSAAATTDNPAASVA
                     IAK"
     gene            1076..1603
                     /locus_tag="VLI90_09765"
     CDS             1076..1603
                     /locus_tag="VLI90_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14537.1"
                     /translation="MASAAPAHVIDALNGLLEAEVNSVFRTINEGSPYLSRATAEVRL
                     PLSECGRLSRQHAQELADLIDSLGGTPMPRQMPRMEDQYLAYLSLKFLLPKLVMEKEL
                     ILRRYENAKRAIGPAFPQVIQQLDRITAEQQEYLDALKKAASDATGGRYNKPAGRNGN
                     SAVSTDNSDAGPTPQ"
     gene            1902..>2686
                     /locus_tag="VLI90_09770"
     CDS             1902..>2686
                     /locus_tag="VLI90_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14538.1"
                     /translation="MRRKNHIEELEARRLLSVSASSLFVFTTGTKLTYQITSSGNTAT
                     STTTVKGAATFNGHNAIEIDGTTSNVAGTSKSFVGLDGAGNAVSYGSSGTTAGGSFTD
                     TNTPPAINFPATMNAGTTYTENWGTHDVFAGGTSDSTTVHTVKLTSASLVSVAVPAGT
                     FNAYLFNETDTITAGGFTLPTSTIQQWIVPNKGLVKSVITSSGNTTTEVLIPNNVVVG
                     PAAKLLIAQQPTTGKPGVALTPAIKVNVTDANGNIVTGNTSTVA"
BASE COUNT          530 a          889 c          812 g          455 t
ORIGIN      
        1 caggtgcacg acctggcgat cgccatgatg cacacaaccg gtgggggcgc gctgctggtc
       61 gttgcgatct tcggcctcat cgtcggcatc ggcttcaaga tatcggccgt gccgttccac
      121 ttctggtgcc cggacgtctt cgaaggcgcc agcatcgacg tgacgacgtt cctctccgtt
      181 gccagcaaag gcgcggggct gatcctgctg ctgcgcatcg tcgagatgct gggcgcggcc
      241 agcgacttca ccgccaacgg cacgctcacc gccattgccg ccgtcatcgg cacgatcggc
      301 gcgattaccg ccaccgtcgg caacaccggc gcgttcgtgc agaagaacat caagcggctg
      361 ctggcctaca gctcgatcgc ccacgcggga tacatgctgt gcgcggtgtc gctgatggtg
      421 aagagccaat ccgcccagcg cgggtacgac cccgccagtt cggcggcgca agccatcctg
      481 ctgtacctgg cgatttatgc gctgatgaac ctgggcgcgt tcaccgtggc cgcgctggtc
      541 gcgcgacaaa gcaacggcgg tgagacgctg gccgacttca ccgcgctcgg ccgccgatca
      601 ccgctgctgg cggcttgcat gttctgctgc ctcatcagcc tgatcggcct gccgccgttc
      661 gccgggttca ccgccaagtg gaacctcctt tgggtcctgt tccagaacgg cgggtggtgg
      721 tgggcgctga tcatcgtcat cggcgtcaac acgatcatca gcgcgttcta ttacttccgc
      781 gtcatccgcg cgatgtacct ggaagacaac gaagcccccg tgttccacgg taacccgctg
      841 ggcgtggcca tcagcgtcgc ctgctcggtc gcgctggtcc tgctgctgct cggtttcagc
      901 ccgctgtcgc ggatgacgaa caactacagc cagctgtacc tttccggtcc aacatcgcca
      961 agcgccgcgg caacaacaga taacccagcg gcgagcgtgg cgatcgccaa atgattgatc
     1021 caaacgaaaa tcccaccctg atgaatcgca ctgacagatg ctaaactccc cgcccatggc
     1081 ctccgccgct cctgctcatg tgatcgatgc gctcaacggg ctgctggaag cagaggtgaa
     1141 cagcgtcttc cgcacgatca acgaaggctc cccgtacctg agccgcgcga cggcagaagt
     1201 ccgcctgccg ctgtccgaat gcggacgcct gagccgccaa cacgcgcagg agctggccga
     1261 cttgatcgac tcgctcggcg gcacaccgat gccccggcaa atgccgcgga tggaagatca
     1321 gtatctggcg tacctgtcgc tgaagttcct gctgccgaag ctggtgatgg agaaggaact
     1381 gatcctgcgg cgctacgaga acgcgaagcg cgcgatcggc ccggccttcc cgcaggtgat
     1441 ccagcagctg gaccggatca cggccgagca gcaggaatac ctggatgcgc taaagaaagc
     1501 cgccagcgat gccaccggcg gccgctacaa caaacccgcc gggcgcaatg gaaacagcgc
     1561 cgtttcaact gacaattccg acgccggccc gacaccgcag taggcgaggg ttgtgagttc
     1621 ggcaaacgct cgcgccggtc ccttcgcccg gcagtccggg agagggtgag ggtgattcac
     1681 atttccagag ccccaggcat atcccggccg ggatctgggt gctcatctcc ctgatcttcc
     1741 tgctgaacct cggcatccgc ttcgcgacgc gggcgcggaa gtaagttctc acatcgccgg
     1801 gaaaatgctg gcaatttgaa aaacccgcgg gtaggttttg ccgctgcagt tgtctgcagc
     1861 acacgtatgc acgcgggaat tcatttcgtg ggagagagat catgcgtcgg aaaaaccaca
     1921 tcgaagaact ggaagctcgt cgtctgctca gcgtcagcgc gtcgtcgctg ttcgtcttta
     1981 cgacggggac taagctgacg taccagatta cctcgagcgg caataccgcc acctccacga
     2041 ccacggttaa gggagcggcg acgttcaacg ggcacaacgc gatcgaaatc gacggcacga
     2101 cttcgaacgt ggccggcaca tccaagagct tcgtcggcct cgacggcgcg ggcaacgccg
     2161 tcagctatgg ctcaagcggc acgaccgccg ggggcagttt cacggacacc aacacgccgc
     2221 cggcgatcaa ttttccggcg accatgaacg cgggcacgac ctacaccgaa aactggggga
     2281 cgcacgatgt gttcgccggg ggcacgtcgg actcgacgac cgtgcacacg gtgaagctga
     2341 cgtccgcgtc gctggtgtcg gtggccgttc cggcggggac cttcaatgcg tatctgttca
     2401 acgagaccga cacgatcacc gcaggcggct tcacgctgcc cacgagcacc atccagcaat
     2461 ggatcgtgcc gaacaaggga ctggtgaaat cggtcatcac cagcagcggc aacaccacga
     2521 cggaagtgtt aatccccaac aacgtcgtcg tcggtccggc cgccaagctg ctgatcgcgc
     2581 agcagccgac caccgggaaa cccggcgtgg cgctgacgcc ggccatcaag gtgaacgtca
     2641 ccgatgccaa cggcaacatc gtcaccggca acacatccac cgtcgc
//
LOCUS       DATIMH010000563         9862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4176723, whole genome shotgun sequence.
ACCESSION   DATIMH010000563 DATIMH010000000
VERSION     DATIMH010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4176723"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1273
                     /locus_tag="VLI90_09775"
     CDS             221..1273
                     /locus_tag="VLI90_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSV14539.1"
                     /translation="MANETLSAPARVSAAAVPVPLALPGRATAAPHVRRPARFRPRPS
                     RRDRWGYLFIAPWVIGFLLFTAGPMIASIALSFTDYDMAAARPVGLGNYADLLWRANP
                     VGGHGDPQLYKSLWNTFYYTAFAVPLGLIGSLLLAVLLNQKLRGLSFFRTVYYVPSLV
                     PAVASSLLWIWILQPEKGLLNQFLIWLFAQWPINALHLAPPAWLQSEAWSKPSLIVMS
                     LWGIGGARMVIFLAGLQGIGEQYYEAAKIDGAGPIRTFRHVTLPLITPMIFFNLVLGV
                     IAASQTFTTAYVMTNGGPNDSTLFYALYLYQSAFERLEMGKASAMAWILFTILLVFTL
                     IQFNRSKKWVHYEGEE"
     gene            1496..2611
                     /locus_tag="VLI90_09780"
     CDS             1496..2611
                     /locus_tag="VLI90_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSV14540.1"
                     /translation="MKRVFLYILALGIAAIFLLPLLWTVSTALKTRQQVFAAPPRWIP
                     IGYRALDANGKWQPVTLVSRIDTAAAEVRLQSGPQAGQTIAVELNVIHNDQGQFYAQL
                     RAIEPVAVDLLRMFHDGVAQVRFPDSPNAVFLEPSELKEVVQPQWSNFAAAWRSLPLP
                     FHWFLLNTYTITAICVIGQTLSCSLVAYGFARFRFHGRGVLFLIMLSTMMLPAQVTMI
                     PMFLIWSRLHAVDTFVPLTLPAFFATSAFSVFLLRQFFLGLPRELDEAAMIDGCGPFR
                     IWWQILMPLCRPAVITVAVLTFLASWDDFAGPLIYLHRLENYTVSLALRMFQDQYGTD
                     YSLIMAAALIHIVPPVVLFFIAQRYFVKGIAMSGLKG"
     gene            3050..4153
                     /locus_tag="VLI90_09785"
     CDS             3050..4153
                     /locus_tag="VLI90_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14541.1"
                     /translation="MSRLIVILVSFLMLIPLSTAALADASAPPTTAPKVLNAPPRVMV
                     YPFTSVGDPGSYAWIGNGIQQSLLVEAGRPGFVTMAAPTTQPAEPAADLAAVAAKAGA
                     AVVVFGSYQILNSDIRCTGQVVDTATGQTITSLSATGQLRELFKLEDDLGRQLHNVLQ
                     PPANALAARDQQQTATQPQESYPPVASSTSGYYYNSGGSDSYVPATYYVPTSTYAYPA
                     YGYDYPYYPYYYPYTTSIIIGSTRFNFCRFDRGRFDCDDRGLRFRGGTGFTRGGFAFS
                     GHVGNVGFAGRAPLTQFNGSRGFVGGGAVGFRGSGGFAGGFGGRAPVMSNPGSIHAVG
                     AGGGMFHGGGNAFGGGGGFVGGRGGGMAMGGHR"
     gene            4453..6294
                     /gene="lpdA"
                     /locus_tag="VLI90_09790"
     CDS             4453..6294
                     /gene="lpdA"
                     /locus_tag="VLI90_09790"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HSV14542.1"
                     /translation="MAAEVTMPQLSDTMTEGTVVKWHKKEGDKVRAGEEIADIETDKA
                     VMPMEAFEGGTLAVIKVKEGEKIAVGGVLGVIASGSEKVEEIKQKYAAGGGGASAAAA
                     PKPAAAEKPAAPSPKSPATAQAIAPSAPAKPTSSPGKYEYDILVIGGGPAGYAAAIRA
                     GQLKKKVLCVERENLGGTCLNWGCIPTKALLEDGAFIRKIRTDAAAHGVSFDNVKIDF
                     AKLIGRSRTIADKLAKGIGSLFHKYGVKSEIGDAQLIAPHRVKVTTKAGTKEITADHI
                     IIAVGAKSTPLPGVAFDGKRVITSREAMTLPQQPKRMAIIGAGAIGCEFADFYNAVGT
                     EIVLVEMLDHLLPNEDDDVARVLERVFDKRKIDIRTKTKTDKVEVTPSGVKLTLSGAK
                     PGVVEADVVLVAIGVTGNADGAVDPKSGLELFRGRVKVTPDYRTNLENVWSVGDCVAL
                     HWPEQSAMGGYRHPDLAHVAHHEAVHLVELIAGVSDHHIDYKQIPGCTYTHPQVASMG
                     YTEKRAREAGYELKIGKFPFSASGRALAAGEPDGFVKLIFDAKFGELLGAHMIGENVT
                     DMLSELVMARKLEATEEEIIDAIHPHPTYSEAIMEAAGVAYGRAIHL"
     gene            6443..8431
                     /locus_tag="VLI90_09795"
     CDS             6443..8431
                     /locus_tag="VLI90_09795"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HSV14543.1"
                     /translation="MKHVLSLVIALLMFLPTKAVAADGDRTSIKIDLAKPGHAISPTL
                     NGVFFEDVNFGGDGGLYPERIKNGSFEFSPDPLMGWRKVERGGGAGQLAVRDDRPLNE
                     SNPHYLHLAIDDIGDGGNGSFGISNEGFRGMGIVKGATYTFSAFARSANAQPITLTIE
                     LQDHANKPIASAQLSGFTDQWQKHSVEIQCPSGDENAHLILSAKLVGAVDLDMVSLFP
                     KDTWNNRPNGLRPDLVQLLKDLKPKFIRFPGGCIVEGRQLTNRYQWKNTIGDIAQRRL
                     IVNRWNMEFKHRQTPDYFQSFGLGFYEYFQLCEDIGARPVPILNCGMACQYNTSELAP
                     LDALDPYIQDALDLIDFANGPVESEWGKRRAAMGHPAPFNLDRIGVGNEQWGPQYIQR
                     YERFAKALKAKYPQVQLIAAAGPNPDGREFDYAWKRLTELGADILDEHYYRKPEWFFA
                     NTHRYDQYDRSKPKVFAGEFAAQSVAIASPENRNDWRCALSEAAFMTGLERNGDVVVM
                     SSYAPLFAHVDAWQWTPDLIWYDNLRSYGTPSYYVQKLFSTNAGSTLLPAEVTGDVVG
                     LYTVASRDDSSGDVIIKAVNAQGNAKPLHVSLNGLSKSSATLDTQGLASADLKAENSL
                     DAPTKIAPVAAAAQQISGSFDPELPPFSLTVFRIHPGN"
     gene            8834..9586
                     /locus_tag="VLI90_09800"
     CDS             8834..9586
                     /locus_tag="VLI90_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14544.1"
                     /translation="MKIKSLVLLVSLVGLIGSSATFAAKTPTKQPGFGNMDGSTGGRR
                     GGGQTKTGNYNDSADPNAAAQSKHDSIANDPNAGMNKGVKDEIAADAAAMDVAQKKVE
                     KQFESSQEWTDGSAEYDKAKAAYDKARSNVLQALKEKPDYKAAASSAEKSSQALSDLR
                     KGGDATPDQLTAAASASFSAQSAVTKMEKDACAADPDVTAAKAKFNDAATAMTALRQK
                     EHAAVLADADYQSAKQKYDAAKSKTASAAKKD"
BASE COUNT         1850 a         3058 c         3171 g         1783 t
ORIGIN      
        1 gttaagacgg gataagtcct gtcaagttgc gggagagctt caggattcca cgcggctgcg
       61 cgaaaatcgg cggtaaaaat ctggcgcggc tcatccccgg atcgactgca aactcgtgac
      121 gaagcgggct ttgctcgata agcttgtgaa cgttggcgcg gcaacgtggt agcaccatcg
      181 gcaactgttc ctccaacgat gcgcagcggc gggttatcgc atggcgaatg aaacgctatc
      241 ggcaccagcg cgggtgtccg cggcggcggt tccggtgccg ctggcgctgc ccgggcgcgc
      301 gaccgccgcc ccgcacgttc gccggccggc gcggttccgc ccccggccca gccggcgcga
      361 tcgctggggg tatttgttca tcgcgccgtg ggtcatcggc ttcctgctct tcaccgccgg
      421 gccgatgatc gccagcatcg cgctgagctt caccgattac gacatggccg ccgcccggcc
      481 ggtcggactc ggaaattatg ccgacctgct ttggcgcgcc aaccccgtcg ggggccacgg
      541 cgatccgcag ctgtacaaga gcctctggaa cacattttat tataccgcgt ttgccgtccc
      601 gcttggattg atcgggtcgc tgctgctcgc ggtgctgctg aaccagaaac tgcgcggcct
      661 ctcgttcttc cgcaccgttt attacgtccc cagcctcgtt cccgccgtcg ccagcagttt
      721 gctttggatc tggatcctgc aaccggaaaa gggattgctg aatcaattcc tgatttggct
      781 gttcgcgcaa tggccgatca atgcgctgca cctggcgccg cccgcgtggc tgcagagcga
      841 agcgtggagc aagccgtcgc tcatcgtgat gagcctttgg ggcatcggcg gggcgcgcat
      901 ggtcatcttc ctcgccggcc tgcaaggcat cggcgaacag tattacgaag ccgccaagat
      961 cgacggagcc ggaccaatca gaacgttccg tcacgtcacg ttgccgctga tcacgccgat
     1021 gatcttcttc aacctggtgc tcggcgtcat cgcggcgtcg cagacgttca ccaccgcgta
     1081 cgtcatgacc aacggcgggc caaacgattc cacgctcttc tacgcgctgt atctttacca
     1141 gagcgcgttc gaacgactag agatgggcaa ggccagcgcg atggcgtgga tcctgtttac
     1201 gattctgctg gtgttcacgc tgatccagtt caatcggagt aagaagtggg tgcattatga
     1261 aggagaggag tgattgctaa gaattcaccc tgagcgcagc acgtacttgt gcgcagtcga
     1321 aggatctggc caaggagttg atgaacgatg acttgcgcaa ctagccgctt gcggcttcgc
     1381 aatccaggcg gggtggattg cgaagccgca agcggctgga gcgcctgtcg cgctagcaac
     1441 ggttgaccga cttcgactcc ggcggaatac cgcctgatct cgcgataagc ccgatatgaa
     1501 gcgcgtcttc ctctacatcc tcgcccttgg catcgcggcg attttcctgc tcccgctgct
     1561 gtggaccgtc agcaccgcgc taaagacccg gcagcaggtg ttcgccgcgc cgccgcgctg
     1621 gatccccatc gggtaccgcg cgctcgacgc caacggcaaa tggcagccgg tcaccctggt
     1681 gtcgcgcatc gatacggcgg cggcggaggt gcgcctgcag agcggcccgc aggccgggca
     1741 gacgatcgcc gtcgaattga acgtcatcca caacgaccag ggccagttct acgcgcagct
     1801 gcgcgcgatc gaacccgtcg ccgtcgatct gctgcggatg ttccacgacg gcgtggcgca
     1861 ggtgcgcttc cccgattcac ccaacgcggt gtttctggaa ccgtcggagc tgaaggaagt
     1921 cgtccagccg cagtggtcga atttcgccgc cgcctggcgg tcgctgccgc tgccgttcca
     1981 ctggttcctg ctcaacacct acacgatcac cgccatctgc gtgatcggcc agacgctcag
     2041 ctgttcgctc gtcgcctacg gctttgcgcg cttccgcttc cacggccggg gcgtgctgtt
     2101 cctcatcatg ctctccacca tgatgctgcc cgcgcaggtg acgatgatcc cgatgttcct
     2161 gatctggagc cggctgcacg ccgtcgatac gttcgtgccc ctcacgctgc ccgcgttctt
     2221 tgccaccagc gcgttcagcg tcttcctgct tcgacagttt ttcctaggcc tgccgcgcga
     2281 actcgacgaa gccgccatga tcgacggctg cggcccgttt cgcatctggt ggcagatcct
     2341 catgccgctg tgccgccccg cggtgatcac cgtcgcggtg ctcacgttcc tcgccagctg
     2401 ggacgatttc gccgggccgc tcatttacct gcatcgcctg gagaactaca cggtcagcct
     2461 ggcgctgcgg atgtttcagg accaatatgg caccgactac agcctgatca tggccgccgc
     2521 gctcatacac atcgtcccgc cggttgtgct gttcttcatc gcgcagcgct atttcgtcaa
     2581 aggcatcgcg atgagcgggt tgaaggggtg aagtcccatc cgttagcggc cgcgtgtaaa
     2641 gggccagtcc tgctgtggcc gtcgctgcga ccgccgcggc gcccgaaccc ggctcgcgga
     2701 caccgctcgg tatcgccgcc gttgtcccct ccgctgccgc ggcccgttca ctcaccagcg
     2761 aaccgccgct ttcttttttg ttggatgagc cgaccccacg ccgggtcgga gcgtaatcgc
     2821 gacgacctgg atcttttctc gcgccacaga atccgggttg tcgggggtac cctggcgaac
     2881 ccggcgtggg gggaagtccc gtcttacaca ctcaacgttc gcgttttgta tgcagattcg
     2941 agcagatccc tctgcacgga ttgcatggag gttcgtgcga aattcaccaa catcggacgt
     3001 tgacgcgggt ctataattgg tctgagcgcc ctgtggtcgg aggtgttgca tgtcacgcct
     3061 aatcgtcatc ctcgtttcgt tcctgatgct gatcccgttg agcactgccg ctttggccga
     3121 tgcgtcggcg ccgccgacga ccgccccaaa agtcttgaac gcgccgccgc gggtgatggt
     3181 ttatccgttc acatcggttg gcgatccggg gtcgtacgcg tggatcggca acggcatcca
     3241 gcaaagcctg ctggtcgagg cggggcggcc ggggtttgtg acgatggcgg cgccaacgac
     3301 gcagccggcg gagcccgccg ccgacctggc ggcggtcgcc gcgaaagcgg gggcggcagt
     3361 tgtcgtcttc ggcagctatc agattttgaa cagcgacatc cgctgcaccg ggcaggtggt
     3421 tgacacggcg acgggtcaga cgatcacgtc gctgagcgca accggccagc tgcgcgagtt
     3481 gttcaagctg gaagatgacc tgggccggca gctgcataac gtgctgcagc cgccggcgaa
     3541 cgcgctggcg gcgcgcgacc agcaacagac cgcgacgcag ccgcaggaga gctatccgcc
     3601 ggtcgcgtca tcgacgtccg gttattacta caacagcggc ggcagcgatt cgtacgtgcc
     3661 ggcgacctat tacgtaccga catccaccta cgcgtatcca gcgtacggct acgactatcc
     3721 gtactatccg tactactacc cctacacgac cagcatcatc atcggctcga cgcggttcaa
     3781 cttttgccgg ttcgaccgcg ggcgatttga ctgcgacgac cgcgggttgc gcttccgagg
     3841 cggcaccggt ttcacgcgcg gcgggttcgc attctccggc cacgtgggca acgtcggctt
     3901 cgccgggcgg gcgccgctga cgcagttcaa cggcagccgt gggtttgtcg gtggtggggc
     3961 cgtcggcttc cgcgggtcgg gcgggtttgc cggtgggttc ggcggtcgcg cgccggtgat
     4021 gtcgaacccc ggctcaatcc acgccgtcgg cgctggcggc gggatgttcc acggcggcgg
     4081 caacgccttc ggtggcggcg gtggtttcgt cggcggccgc ggtggtggca tggcgatggg
     4141 cgggcatcgg tgagtgactt caaagataga cgcgacgcga tgacgcgaaa gcgcaaagac
     4201 agacgcgaag aagattgaga acgacgtcga gctttattca atctggcgat ggacgcacgg
     4261 gccggaggca gccttcggcc cgtgcgtgta tcgctgagcg taagaacctt tccacatcaa
     4321 tcctgcctgc atttccttcg tggccgcctt cgcgtccttc gtgccgtcgc gttcgcattc
     4381 ccgccccgca gtgacaaccg gctacctgtt ccatatcatc gcacgccctc aatttgccgg
     4441 gagatcaaac gaatggccgc tgaagtgacg atgccgcagt tgtccgacac gatgaccgaa
     4501 ggaaccgtgg tcaaatggca caagaaggaa ggggacaagg tgcgcgccgg cgaggagatc
     4561 gccgacattg aaaccgacaa ggcggtcatg ccgatggagg cgttcgaggg cggcacgctg
     4621 gcggtcatta aggtgaagga gggggagaag atcgcggtgg gcggcgtgct gggggtgatc
     4681 gccagcggca gcgagaaggt cgaagagatc aagcagaagt acgccgcggg cggcggcgga
     4741 gctagtgctg ccgcggcgcc gaaaccagcg gcggcggaga agccggcggc tccatcacca
     4801 aaatcgcctg ccaccgcgca agcgatagcg ccctcagcgc cggcgaaacc gaccagcagt
     4861 cccggcaaat acgaatacga catcctcgtg atcggcggcg gtcccgccgg ttacgcggcg
     4921 gcgattcgcg ccggtcaact caagaagaaa gtcctctgcg tcgagcgcga gaacctcggc
     4981 ggcacctgcc tgaactgggg ctgcatcccg accaaggcgc tcctggagga cggcgcgttc
     5041 atccgcaaga tccgcaccga cgcggcggcg cacggcgttt cgttcgacaa cgtgaagatc
     5101 gattttgcca agttgatcgg ccggagccgg acgatcgccg acaagctggc caagggcatc
     5161 ggcagcttgt tccacaagta tggcgtgaag agcgaaatcg gcgacgccca gctgatcgcg
     5221 ccgcatcgcg tgaaggtgac gaccaaggcg ggcacgaagg agatcaccgc cgatcacatc
     5281 atcatcgccg tgggcgcgaa atcgaccccg ctgccgggcg tggcattcga cggcaagcgc
     5341 gtgatcacca gccgcgaggc gatgacgctg ccgcagcagc ccaagcggat ggcgatcatc
     5401 ggcgcgggcg cgatcggctg cgagttcgcc gacttttaca atgcggtggg caccgagatc
     5461 gtgctggtcg agatgctcga tcacctgctg cccaacgagg acgacgacgt tgcccgggtg
     5521 ctggagcgcg tgttcgacaa gcgcaagatc gacatccgca cgaagaccaa gaccgacaag
     5581 gtcgaggtca cgcccagcgg cgtgaagctg acgctctccg gggccaagcc cggcgtggtt
     5641 gaagcggatg ttgtgctggt cgcgattggc gtgacgggca acgccgacgg cgcggtggat
     5701 ccgaaatcgg gtttggaatt gttcaggggc cgcgtgaagg tgacgccgga ttatcgcacc
     5761 aacctggaga acgtgtggtc ggtgggcgat tgcgttgcgc tgcactggcc ggagcaatct
     5821 gcgatgggcg ggtatcgcca tcccgacttg gcccacgtcg cccatcacga ggcggtgcac
     5881 ctggtcgagc tgatcgccgg cgtcagcgat catcacatcg actacaagca gatccccggc
     5941 tgcacctaca cgcacccgca ggtggcgagc atgggctaca ccgagaagcg cgcccgcgaa
     6001 gcgggatacg aattgaagat cggcaagttc cccttcagcg ccagcggccg cgcgctggcc
     6061 gcgggggaac cggacgggtt cgtgaagctg atcttcgacg ccaagttcgg cgagctgctg
     6121 ggcgcgcaca tgatcggcga aaacgtgacc gacatgttga gcgagctggt gatggcgcgg
     6181 aagctggagg cgacggaaga ggagatcatc gatgcgatcc acccgcaccc gacgtacagc
     6241 gaagccatca tggaagccgc gggcgtggcg tatgggcggg cgattcatct gtgactccgt
     6301 gatagccgcg ggcgacagcc agcgggtctc ttgaaatcac cgcggctgaa tgtctcgcgc
     6361 ccccgctggc tgtcggctgc ggctagcacg cgcggtcatc cgcggcttgt cattcgctct
     6421 tggcgcgttc aaactttggc gcatgaagca cgtcctctcg ctcgtcatcg cgttgctgat
     6481 gttcctgccg accaaagcgg ttgccgctga tggcgatcgc acttccatca agatcgatct
     6541 ggccaagccc ggtcacgcga tcagccccac gcttaacggc gtcttcttcg aagacgtcaa
     6601 cttcggcggc gacggcgggc tttatcccga gcgcatcaaa aacggctcat tcgaattctc
     6661 gcccgatcca ttgatgggtt ggcggaaggt cgaacgcggc gggggcgccg ggcaattggc
     6721 cgtgcgcgat gaccgcccgc tgaacgaaag caacccgcat tacctgcacc tggcgatcga
     6781 cgacatcggc gacggcggca acggatcgtt cggcatcagc aacgaaggtt tccggggcat
     6841 gggcatcgtc aagggggcga cgtacacgtt ctccgccttc gcgcgcagcg ccaatgcgca
     6901 gccgatcacg ctgacgattg aactgcagga ccacgcgaac aagccgattg cgtcggcaca
     6961 gctgagcggg ttcaccgatc agtggcagaa gcattcagtc gagatccaat gtcccagcgg
     7021 cgatgagaac gcgcacctga tcctgtcggc taagctcgtc ggcgcggttg atctggacat
     7081 ggtctcgctg ttcccgaagg acacgtggaa caatcgcccc aacggccttc ggccggacct
     7141 ggtgcagctg ctgaaggacc tgaagccgaa gttcatacgc ttccccggcg ggtgcatcgt
     7201 cgaggggcgg cagctgacca atcgctatca atggaagaac acgatcggcg acatcgcgca
     7261 gcggcggttg atcgtcaatc ggtggaacat ggagttcaag caccgccaga cgccggatta
     7321 tttccagtcg ttcgggctcg ggttctacga atattttcag ctgtgcgaag atatcggcgc
     7381 gaggcccgtg ccgattttga attgcgggat ggcgtgccag tacaacacgt cggagctggc
     7441 gccgctggat gcgctggatc cgtacatcca ggatgcgctg gacctgatcg attttgccaa
     7501 cggcccggtc gaaagcgaat ggggcaagcg ccgggcggcg atggggcatc cggcgccgtt
     7561 caacctcgat cgcatcggcg tcggcaatga acagtggggg ccgcaataca tccagcgata
     7621 cgagcggttc gccaaggcgc tgaaggcgaa gtacccgcag gtacaactga tcgccgcggc
     7681 cggaccgaac ccggatgggc gcgagtttga ttatgcatgg aagcgattga cggagctggg
     7741 ggcggacatt ctggacgagc attactatcg aaagcccgag tggttcttcg ccaatacgca
     7801 ccgctacgac caatacgacc gtagtaaacc caaggtattt gccggcgaat tcgcggcgca
     7861 gagcgtcgcc atcgccagtc ccgaaaaccg caacgactgg cggtgcgcgc tgtccgaagc
     7921 cgcattcatg acgggactcg aacgtaacgg cgacgtggtc gtgatgtcat cgtacgcgcc
     7981 gttgttcgcg cacgtcgatg cgtggcaatg gacgccggat ttgatctggt acgacaacct
     8041 ccgctcgtac ggcacgccga gctattacgt gcagaagctg ttcagcacca acgcgggatc
     8101 aacgctgttg ccggcggagg tgacgggcga cgtcgtcggc ctgtataccg tcgcgtcgcg
     8161 cgacgattca tcgggcgacg tgatcatcaa agcagtgaac gcgcagggga acgccaagcc
     8221 gctgcatgtg tcgctgaatg gattgtcgaa gtcgagcgcg acgctcgata cgcaggggct
     8281 ggccagtgcc gatctgaagg ccgagaacag cctcgatgcg ccaacgaaga tcgcccccgt
     8341 cgccgccgcg gctcagcaaa tcagcgggtc gttcgatccg gaactaccgc ccttttcgct
     8401 gaccgtattt cgcattcatc ccgggaatta atcgcgattt acagccggtg gcgaatcgtc
     8461 ggcagtttct aatcgtgcaa gattgtcgtg acggcggggt atctcctggt gacacaggag
     8521 ctacccatga cccgcatgac ttcgatcttc gcctcgatgt tcgccgccgc gatagttggt
     8581 tccggcgatg gattctccta cacgcactga tgcagatgtg cctgccgcga cggtacaatc
     8641 gccgcaggcg tgataatcct cctccgcacc ctgccctgcc acgcgcgcgc cacgccgtgg
     8701 tggagcgggc ttttttcctg cgatcggcgc tcatttgcgc aaaagatgct tggcaacatc
     8761 gcattgcccc acgataatcc ttcacggccg cacgtgcctc gcccttgccg accgaacccc
     8821 gcggaggatc gtcatgaaga tcaagtctct cgtgttgctg gtcagtctcg ttggtctgat
     8881 tggctcaagc gccacgtttg ccgccaagac gccgacgaag cagccgggct ttggcaacat
     8941 ggacggctcc accggtggcc gccgcggcgg tgggcagacg aagaccggca attacaacga
     9001 ctcggccgat ccgaacgccg cggcgcagtc gaagcacgat tcgatcgcca acgaccccaa
     9061 cgcgggcatg aacaagggcg tgaaggacga gatcgccgcc gacgccgcgg cgatggacgt
     9121 ggcgcagaag aaggtcgaga agcagttcga atcgtcgcag gaatggaccg acggctccgc
     9181 cgaatacgac aaagcgaagg cggcgtatga caaggcgcga tccaacgtgc tgcaggcgct
     9241 gaaagaaaag ccggattaca aggccgccgc gagcagcgcg gagaagagca gccaggcgct
     9301 gagcgacctg cgcaagggcg gggatgcgac gcccgatcag ttgacggcgg ccgcgtcggc
     9361 gtcgtttagc gcgcaatcag cggtaacgaa gatggagaag gacgcctgcg ccgccgatcc
     9421 cgacgtcacg gccgcgaagg ccaagttcaa cgatgccgcc acggcgatga cggcgctgcg
     9481 gcagaaggaa cacgccgccg tgctggcgga tgcggattat caatcggcca agcagaagta
     9541 cgacgccgcc aagagcaaga cggccagcgc cgcgaagaaa gactaagcgg cgcttggaat
     9601 ggtcaaccgc tgattggtaa atcgactcac aaaccgagcc acgtcgggtt cggagcgtaa
     9661 ttgcgacgaa gcgagattct gcggcgcgcg agaaacatcc gggtcgtcgg agtaccttcg
     9721 aacccggcgt ggtaaccaat gcatcggttg ctacttcgca agctgcggaa cgtccgataa
     9781 tccgcattcc ctccctcgaa atatcccgct tgcgccgcgg gtgatcacgg tataatttct
     9841 gcacacctgt gtggtctttt gt
//
LOCUS       DATIMH010000564         4838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3887172, whole genome shotgun sequence.
ACCESSION   DATIMH010000564 DATIMH010000000
VERSION     DATIMH010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3887172"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2436)
                     /locus_tag="VLI90_09805"
     CDS             complement(<1..2436)
                     /locus_tag="VLI90_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HSV14545.1"
                     /translation="MFRIVGFGQSVLLGLVTLCGGATALFGYTPAQSNRVVINFDRDW
                     KFIREDVPGADKPEFNDAAWADVGLPHTYNDQDTYDKPSDPDHDKKTTAAPKKPKEPK
                     PGPAAGGAAGAPDPGPKTIDQDPTANDRQFNSAGGDGGWGGKTWYRKHFTLDPQYRDR
                     KIYIEFEAARTVAEVYINGQHLGRYASGFSSFAYDLTPYVSFDKPNVLSVQIDDTDDE
                     NLAAGNRSHWQPVLGGLYRNVYLFVTDKLHVTLPYYSDLKTEGTYVATPKVSQQSADF
                     HITTEIRNAYDKPVTANLATEVVDRAGKVVLTLKDQKEIPAGGLIKFDQSSPIRRPHL
                     WSLDDPYLYTVYSTVLNGDAVADVYTTPLGVRTIGFDHAHSRFQINGEEVLLHGWGQK
                     PENSWPGLGSALPDWLLAADMQMMKDGGGNIVRWGHCAGPTAELDAGDRIGYLNWQAN
                     LSGESDYKGSVWEGKLIATRDIIIRDRNHPSICWWEGNNRDLSVDHTNLMKAVVAKWD
                     WMSPRPFANRRPYSKATAPLQDFSMEENGSKNSVPGKAMVEAEFYRPETDRRAWDLDS
                     PPFKACTAEYWRTEFQAMREIVKDWKGISQRGGGVKWHFTDDTTHGRLGAYSMARLSG
                     SLDATRLPKDIYYGLKVLYSEPSQPQIHIMGHWNYEQTKKHTIDVYANTDEVELLING
                     RSLGRKTINDWRATWEEVPFEPGVIKAIGYNAGSPDAFDERHTAGPPARIQLEVMTDP
                     NGMKADRADVAIITASVLDRDGNVHPLAENDITFAVTGPGNYRGGANMAIPNTTGKRT
                     LKAEAGKIRVSVRS"
     gene            complement(2567..2851)
                     /locus_tag="VLI90_09810"
     CDS             complement(2567..2851)
                     /locus_tag="VLI90_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14546.1"
                     /translation="MPREFGKLKRATLRARVGWSVRRRVFVLMSGGSFHGGIPSVAIL
                     PPSNDCTRGAASCSTARPKLKQCLSAIGNDEAQQFSVRGIERHYRIAERH"
     gene            3051..4319
                     /locus_tag="VLI90_09815"
     CDS             3051..4319
                     /locus_tag="VLI90_09815"
                     /inference="COORDINATES: protein motif:HMM:NF027479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HSV14547.1"
                     /translation="MKNDAQSQISTSEENETVNRKMLRPAVAATVAALVSLAPGHRAS
                     AATVTVKNDADIARPSETVEVPWSDLEKGLPNNAKADRVVVSDNSGKEIQAQPIYFNG
                     QKKPAEMLVFQADLGPGESKTFTIKPGEPKPYEPKVFGRWVPERNDDFAWENDKVAFR
                     IYGPTLEIVEPASSGVDTWQKRTPNLVIDKWYQLAQSINSGYYHYDHGEGMDGYKVGH
                     SQGDGGTALWLDGKRFTTGIKGWKKWNVIANGPIRLIFDLTYDPIDVNGNSVRELKRI
                     TLDAGRHLNHFQSTFTSDKADDKLQALAGLGEHQDRPFEKQINKDKGWVTIWDAADNP
                     GEKKPTPDAPSGHVGVAVVMPASQVMDAFEAEHHLELSTKLTPGQPVSFWAGSCWDKG
                     GDFASYDDWNKYIDECAKRVASPLKVSVSQ"
     gene            4369..>4838
                     /locus_tag="VLI90_09820"
     CDS             4369..>4838
                     /locus_tag="VLI90_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14548.1"
                     /translation="MLRRAPLLSLALLILASSLAHAADNDDIALLKKAADWQIAALPT
                     QKAYKQDTANGWIRAPFYVGVMSLHDALKDDQHYLNAMMDIAEQCHWELANRKYPPGG
                     TTVPSSWSTTQAALDAGILEPGRPFLRHADDLAMGQLYCELYFIKRDPHMIESL"
BASE COUNT          918 a         1561 c         1453 g          906 t
ORIGIN      
        1 cgaacgcacc gatacgcgga tctttcccgc ctcggctttg agcgttcgct tgccggtcgt
       61 gttcgggatc gccatgttcg cgccgccgcg gtagttgccg gggccggtga cggcgaacgt
      121 gatgtcgttt tccgccagcg ggtgaacgtt gccatcgcga tccagcacgc tggcagtgat
      181 gatcgccacg tcggcgcggt cggctttcat gccgttggga tcggtcatca cttccagctg
      241 aatccgcgcg ggcgggccgg cggtgtggcg ttcgtcaaac gcatcgggcg aacccgcgtt
      301 gtaaccgatc gccttgatca cgcccggttc gaacggcacc tcttcccagg tggcgcgcca
      361 atcgttgatc gtcttgcggc cgagcgatcg cccgttgatc agcagctcca cctcgtccgt
      421 gttggcgtag acgtcgatcg tgtgcttctt ggtctgctcg taattccagt gacccatgat
      481 gtggatctgc ggctgcgacg gttcgctgta cagcaccttc aggccgtaat aaatgtcctt
      541 cggcaggcgc gtcgcgtcga gcgaaccgct gaggcgcgcc atagaatatg cgccgagtcg
      601 gccgtgcgtg gtgtcgtcgg tgaagtgcca tttcaccccg ccgccgcgct ggctgatgcc
      661 tttccagtct ttgacgatct cgcgcatcgc ctggaactcg gtccgccaat actcggcggt
      721 gcacgctttg aacggcggtg agtccaggtc ccacgcgcgg cgatcggtct cgggccgata
      781 gaactcggct tcgaccatcg ctttgccggg cacggaattc ttcgacccgt tctcctccat
      841 cgaaaaatcc tgcagcggcg cggtggcctt ggaatacggc cggcgattgg cgaacggccg
      901 gggggacatc cagtcccact tggcgacgac cgctttcata aggttcgtgt gatcgaccga
      961 caggtcgcgg ttattgcctt cccaccagca gatcgacggg tgattgcgat cgcgaatgat
     1021 gatgtcgcgc gtcgcgatca gcttgccttc ccagacagaa cccttgtagt cgctttcgcc
     1081 cgagaggttc gcctgccagt tgagatagcc gatgcgatcg cccgcatcga gttccgcggt
     1141 gggaccggcg cagtgacccc agcggacgat gttgcccccg ccgtccttca tcatctgcat
     1201 gtcggcggcc agcaaccaat ccggcagcgc cgaaccgagc cccggccagc tgttttccgg
     1261 cttctggccc cagccgtgca gcagcacttc ttcgccgttg atctggaatc ggctgtgtgc
     1321 gtgatcgaat ccaatcgtcc gcacgcccag cggcgtcgta tagacgtcgg cgacggcgtc
     1381 gccattcaga actgtcgaat aaacggtgta cagatacgga tcatccagtg accacaaatg
     1441 cggccggcga atgggcgagg actgatcgaa cttaatgagc ccacccgccg gaatttcctt
     1501 ctggtccttc agcgtcagca cgaccttgcc ggcgcgatcg accacttccg tcgccaggtt
     1561 cgccgtcacg ggcttgtcgt atgcgttgcg aatctcggtc gtgatgtgga agtccgccga
     1621 ctgctgcgag accttgggcg tggcaacgta ggtgccttcg gttttcaggt cgctgtaata
     1681 cggcagcgtc acgtgcagct tatcggtgac gaacaaataa acgttgcgat acagcccacc
     1741 cagcaccggc tgccaatgac tgcggttgcc cgcggccagg ttttcatcgt cggtgtcgtc
     1801 gatctgcacc gacagcacgt tcggcttgtc gaacgaaacg tacggcgtca ggtcgtaagc
     1861 aaagctgctg aaccccgacg cgtatcgccc caggtgctgg ccgttgatgt acacctcggc
     1921 caccgtgcgg gcggcttcga attcgatgta gatcttgcgg tcgcgatatt gcggatcgag
     1981 cgtgaaatgt ttgcgatacc aggtcttccc gccccacccg ccgtcgcccc cggcggagtt
     2041 gaactggcgg tcgttggcgg ttggatcctg atcgatcgtc ttcggcccgg gatcaggcgc
     2101 ccccgccgcc ccccccgccg ccggcccggg cttgggctcc ttcggctttt tcggcgccgc
     2161 cgtcgtcttc ttatcgtgat ccggatcgct cggcttgtcg taggtgtcct ggtcgttgta
     2221 cgtgtgcggc aggccgacat ccgcccaggc ggcgtcgttg aactccggct tatccgcgcc
     2281 cggcacatct tcgcgaatga atttccagtc gcggtcgaag ttgatgacga cgcgattgct
     2341 ctgcgccggc gtgtagccaa acaacgccgt ggcgccgccg caaagggtga ctaaacccag
     2401 cagaaccgac tgaccgaaac ccactatccg aaacatcaaa cctccgcatg gatggcaggc
     2461 gcacgataat cgggcggctg cgctttgtac taaaccatta cgttcaaagc gcctatatgt
     2521 tgagcggatc taccttgccg gcacgggaag aacggcgcgc cagacatcag tgtcgctcgg
     2581 cgatgcggta gtggcgctcg atgccgcgca cgctgaactg ttgcgcctca tcatttccaa
     2641 tagctgacag acattgcttt agcttcgggc gggcggtcga gcagcttgcc gcgccgcggg
     2701 tacaatcgtt tgacggcggc aatatcgcga cgctcgggat gccgccatga aatgatcctc
     2761 cgctcatcaa aacaaagacg cgccgcctga ccgaccaccc cacccgcgca cgtagcgtcg
     2821 cgcgctttag tttgccgaac tcgcgcggca aatgaaagac acttcacgga aattcacacg
     2881 cccggcacgg gctgggcggc gccatccacc tccaccaggg aagtgcgctc attgttggcg
     2941 tcgcgtttga atcttgcggc cggggcgttt gggccgttag agtccctccc caactaacgc
     3001 gctgcccgcg gatcgaacgc gtcaacttcc gccggcgctg gtggtatcag gtgaagaacg
     3061 acgcgcaatc gcagatttcg accagcgagg agaacgaaac cgtgaaccgc aagatgctcc
     3121 gcccggctgt cgccgccacc gtcgccgccc tggtctccct ggcccccggc catcgggcga
     3181 gcgcggcgac cgtcaccgtg aagaacgacg ccgacatcgc ccgcccgtcc gaaacggtcg
     3241 aggtcccgtg gagcgatctg gagaaggggc tgccgaataa tgcgaaagcc gatcgcgtcg
     3301 tcgtcagcga caacagcggg aaggaaatcc aggcgcagcc gatctatttc aacggtcaga
     3361 agaaaccggc ggagatgctg gtctttcaag ccgacctcgg ccccggtgaa tccaagacgt
     3421 tcacgatcaa acccggcgag cccaagccgt acgagccgaa agtttttggc cggtgggtcc
     3481 ccgagcgcaa cgacgacttc gcctgggaaa acgacaaggt cgcgttccgc atctacggcc
     3541 ccacgttgga aatcgtcgaa cccgccagca gcggcgtgga cacctggcaa aagcgcacgc
     3601 cgaacctcgt gatcgacaag tggtatcaac tcgcgcagag catcaacagc ggttactacc
     3661 actacgacca cggcgaaggc atggacggtt ataaggtcgg tcacagccag ggcgacggcg
     3721 gcaccgcgct gtggctcgac ggcaagcgct tcaccaccgg catcaaaggc tggaagaagt
     3781 ggaacgtgat cgccaacggt ccaatccggc tcatcttcga cctgacctac gatccgatcg
     3841 acgtcaacgg caacagcgtg cgcgagctca agcgcatcac gctcgacgcc ggtcggcatc
     3901 tcaaccattt ccaaagcacg ttcaccagtg ataaggcgga cgacaagctg caggcgcttg
     3961 ccggccttgg tgagcaccag gaccggccat tcgagaagca gatcaacaag gataaaggct
     4021 gggtcacgat ttgggatgcc gccgacaatc ccggcgagaa gaagccgacg ccggatgccc
     4081 ccagcggcca cgtcggcgtc gccgtggtga tgccggcgag ccaggtcatg gacgcgttcg
     4141 aagccgagca tcacctggag ctatcgacga agctgacgcc ggggcaaccg gtcagtttct
     4201 gggccggctc ctgctgggac aagggcggcg acttcgccag ctacgatgac tggaacaagt
     4261 acatcgacga gtgcgccaag cgcgtcgcgt cacccctgaa ggtttcggtc tcgcagtaac
     4321 catcaaagcc cacggatctg ccgtgggacc atccggagat tttccttcgt gctccgtcgc
     4381 gccccactcc tctccctcgc actgctgatc ctggcatcct cgttagcgca cgccgccgac
     4441 aacgatgaca tcgcccttct aaagaaagcc gccgactggc aaatcgccgc gttgcccacg
     4501 caaaaggcat ataaacaaga caccgccaac ggctggatcc gcgccccgtt ctacgtcggc
     4561 gtgatgtcgc tgcacgacgc gctcaaagac gaccagcatt acttgaacgc gatgatggac
     4621 atcgccgagc agtgccactg ggaattggca aaccgcaagt atccacccgg cgggacgacg
     4681 gtgccatcga gttggtccac aacgcaagcc gcgctggacg cgggcatcct cgaacccggc
     4741 aggccgttcc tgcgtcatgc cgacgacctg gccatgggcc agctctattg cgagctgtat
     4801 ttcatcaagc gcgacccgca catgatcgag tcgctgca
//
LOCUS       DATIMH010000565         4436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2156620, whole genome shotgun sequence.
ACCESSION   DATIMH010000565 DATIMH010000000
VERSION     DATIMH010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156620"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VLI90_09825"
     CDS             complement(<1..1601)
                     /locus_tag="VLI90_09825"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HSV14549.1"
                     /translation="MGGVNLQQLTSQASVGESFATWGKVATVLEEHRMPPKGMPQPAD
                     DERQNAAKWIRTELATYFKKHDGDPGRVTVRRLTSGEYAYAIHDLTGIDPDTGVDASS
                     DSVGGEGFTNFGDVQFMQDANLERYLAAAKKIANHAVIGSGPLEFYVDPGKTGFELSA
                     ITRIKNIYGTYGFRTVSGEGGIPFGLDKYGKAFYVAWRYKNRVGLGEPNATLKDLAAQ
                     QGVSLKFSQHIWEVVNRTGLSYPSSEMVARWQKLPTAGGTDPTVIAAAAKLGCDDLQK
                     YLTTWPSWLFGRGDVAAGGAGDESPLVFNDNSLKAEAVHHFLFNRARFGGGGRGAAAP
                     AGPPKQLLNVAVLNPSASGKPVIIWRNPTVGFRAAGAPGGRGGRNPGTQNGGGAVTPP
                     VANAAAAGQNAGGAIKPPVANAAAAGQTAAVVLPAGGGRGFQPAMPRIPLRAAISEET
                     AKRLNFGHSPDGSTVGPDDFATEGSVAFELVVPEGQTLADFMVDAAVGADRDQVFRIT
                     LSDREEGPRGIPTRTILGDPASAGYR"
     gene            complement(1871..3955)
                     /locus_tag="VLI90_09830"
     CDS             complement(1871..3955)
                     /locus_tag="VLI90_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSV14550.1"
                     /translation="MSALPNVSRRGFLQSATGLLLAFRLSEKAEAAGASKLNAFVHVG
                     ADDSVTLFIHKAEMGQGTVTSLSMLLAEELECDWKKVRIEFPGVNREFGPMQGVFGSQ
                     SVRTSYDSLRKAGAAAREMLVQAAAAKWGIAPSACRAENNTVVNTATNARLSYGSLAE
                     AASKFAPPASPALKDPAQYRLIGKPLKRLDTPSKVDGSAQFGMDVRLPGMLYAVVARC
                     PVFGGKVKSFDATKAKAVAGVKSVVQISNGVAVVADNTWNAMQGRRVLDVQWDEGAVA
                     AMSTPALTKIFQERMQQPGAVARQDGDAPAAIAAAARKIEADYEVPFLAHAPMEPLNC
                     TADVKADHCSVWASTQGQSAAAGIAAQVTGLPPEKIDVHTLYMGGGFGRRAAADYIGE
                     AVEVSKLTGAPIKLTWSREDDLQQDLYRPASYTRFAGALDAEGWPLAITSRIACPPFG
                     GVRNGLSRVGVEGVADVVYAMPHMLVDYHAVEAGIPVSYWRSVGYSQNTFFAESFLDE
                     MAAAGGKDPVEVRRRLLAKSPRMLGVLELVAEKSGWSKPPAAGRARGVSLVNNIGTFT
                     AQVAEVSVAGGKLKIHKVVCAVDCGRVVNPAILEQQIQSGIVFGLAAALKGGITIDRG
                     RVQQSNFHQYDVLRIDEMPLVEVHIVNSQNPSGGVGEASTPGIAPAVCNAIFKATGKR
                     IRRLPLRPADLA"
     gene            complement(3952..4407)
                     /locus_tag="VLI90_09835"
     CDS             complement(3952..4407)
                     /locus_tag="VLI90_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSV14551.1"
                     /translation="MNGKAQTVDVPPAMPLLWVLRDTLGMTGTKFGCGMSLCGACTVH
                     LNGQPVRSCVTPISTLAGKSVTTIEGLSPDKTNPIQQAWIAEDVPQCGYCQSGQIMTA
                     AALLAKTANPTDADIDEAMRNNICRCGTYQSIRRAVHLAAELRAKGGAR"
BASE COUNT          718 a         1438 c         1457 g          823 t
ORIGIN      
        1 cgatacccgg cgctcgctgg gtcgccgagg atggtacgcg tgggaatgcc gcgcgggcct
       61 tcctcgcggt cactgagcgt gatacggaaa acctgatcgc ggtctgcgcc aacggcggca
      121 tccaccatga agtcggccag cgtctgccct tcggggacga caagttcgaa ggcgacagat
      181 ccctcagtcg cgaagtcgtc tggaccgacc gtgctgccgt caggactatg tccgaagttc
      241 aggcgtttcg cggtctcttc gctgatcgcc gcgcgcaacg gtatgcgcgg catcgcgggt
      301 tggaaaccgc ggccgccacc ggcgggcagg acgaccgcgg ccgtctggcc ggcggcagca
      361 gcgttggcca cgggcggctt gattgcgcct ccggcattct ggccagcggc tgccgcattc
      421 gcgaccggcg gcgtaaccgc gccaccgccg ttctgagtgc cgggattacg tcctccgcgt
      481 ccgccgggcg cgcccgcggc gcggaagccg accgtcggat tccgccagat gatcaccggc
      541 ttgcccgaag cgctgggatt gagcacggcc acgttgagga gttgcttggg cgggccggca
      601 ggcgccgcgg cgccgcggcc gccgccgccg aagcgtgcgc gattgaacag aaagtggtgg
      661 accgcttcgg ctttcagtga attgtcattg aacaccagcg gactctcatc gcccgcgccg
      721 cccgcggcca catcgccgcg gccgaaaagc cagctcggcc aggtggtgag atatttctgg
      781 aggtcatcgc agccgagctt cgccgccgcc gcaatcaccg tcgggtcggt cccgcccgcc
      841 gtggggagct tctgccagcg cgccaccatt tccgacgaag ggtagctcag ccccgtccga
      901 ttcacgacct cccagatgtg ctggctgaat ttcaggctga cgccttgttg cgccgcgaga
      961 tccttgagcg tcgcgttagg ctcgccgagc ccgacgcgat tcttgtagcg ccacgccaca
     1021 taaaacgcct tgccgtattt gtccagaccg aagggtatac cgccttcgcc cgacacggtc
     1081 cggaagccgt acgtgccgta gatgtttttg atgcgcgtaa tcgccgaaag ctcgaagccc
     1141 gtcttgcccg ggtcaacgta aaattccagg ggaccgctgc cgatcacggc gtggttcgca
     1201 atcttcttcg cggccgccag atagcgctca agattggcgt cctgcatgaa ctgcacgtcg
     1261 ccgaaattcg taaaaccctc gcctcccacg gaatcgctgg aggcgtccac cccggtgtcg
     1321 ggatcaatcc cggtgaggtc gtgaatcgcg tacgcgtatt cgccgctggt gaggcggcgc
     1381 acagtgaccc ggccgggatc gccatcgtgc ttcttaaagt acgtcgcaag ctcggtgcgg
     1441 atccatttcg cggcgttctg acgctcgtcg tcggcaggct gaggcatgcc cttggggggc
     1501 attcgatgct cctcgagcac ggtcgcgacc ttgccccaag tggcgaaact ttcgcccacc
     1561 gaggcctgcg atgtcaattg ctgcaggttg acaccaccca tgggtgtagc gccagcgtgg
     1621 cactgcacac agtatttggc caccagtggc gccggattcg gcggcgcctc gtccgctccc
     1681 cgcaaagtgc tgccaatcaa tacggccaaa agtgcgctag tgcacgccaa aagcgctacc
     1741 cggcgctttc taatccctct atcaaaatct cggctacggg gagacttcat attcttgtgg
     1801 tgttattcta cgcctgaagg ttaccggtgt ccatgccatc ccttgcaggt ggcagagcac
     1861 cacgttgaag ttatgccagg tccgccggtc gcagcggaag gcgcctgatg cgcttgccag
     1921 tggccttgaa gatggcgttg cagacggccg gagcaatccc cggagtgctg gcttcgccaa
     1981 cgccgcccga gggattctgg ctgttcacga tgtggacttc caccaacggc atctcatcga
     2041 tgcgcagcac atcgtactgg tggaaattgc tctgctgtac gcggccccgg tcgatggtga
     2101 tgccgccctt gagggcggcg gccaaaccga atacgatgcc gctttgaatc tgctgttcga
     2161 ggatggcggg gttgaccacg cggccgcaat ccaccgcgca gaccaccttg tggatcttga
     2221 gcttgccgcc ggcgacggag acttccgcca cttgcgctgt gaatgtgccg atgttgttca
     2281 ccagcgacac gccgcgcgcg cggccggctg cgggaggctt gctccagccc gatttttcgg
     2341 cgaccagttc gagcacgccg agcatgcgcg gcgatttggc cagcagacgg cgccgcactt
     2401 ctaccgggtc cttccccccg gcggccgcca tctcatcgag gaagctctcg gcgaagaaag
     2461 tgttctgcga gtagcccacg ctgcgccagt agctgaccgg aatgccggcc tccaccgcgt
     2521 gataatccac gagcatgtgc ggcatcgcgt agacgacatc ggcgacgcct tccacgccga
     2581 cgcgggagag tccattgcgc acgccgccga acggcgggca ggcgatacgg ctggtgatgg
     2641 cgagcggcca accctcggca tcgagcgcgc cggcgaagcg cgtgtaggac gcggggcggt
     2701 atagatcctg ctgcaggtcg tcctcgcgcg accatgtgag tttgatgggc gcgccggtga
     2761 gcttcgaaac ttcgacggct tcgccgatgt aatcggcggc ggcgcggcgg ccgaagcccc
     2821 cgcccatgta taaggtgtgt acgtcaatct tctcgggcgg aagtccggtc acttgggctg
     2881 cgatcccggc cgcagcgctc tggccctggg tggaagccca caccgagcag tggtccgcct
     2941 tcacgtcggc ggtgcaattg agcggttcca tcggcgcgtg ggcgaggaac gggacctcgt
     3001 aatcggcctc gatcttacgc gcggctgcgg cgatcgcggc tggcgcgtcg ccgtcctggc
     3061 gggccacggc gccgggttgc tgcatgcgct cctggaagat cttggtgagc gcgggagtgc
     3121 tcatggcggc gaccgcgcct tcgtcccact gcacgtcgag cacacgccgt ccctgcatgg
     3181 cattccacgt gttgtcggcg accacggcga cgccgttcga aatctgcacg acgctcttca
     3241 cgccggcgac agccttggcc ttcgtggcgt cgaagctctt cactttgccg ccgaaaacgg
     3301 ggcagcgcgc caccacggcg tagagcatcc ccggcagtcg tacgtccatg ccgaattggg
     3361 cgctcccatc gactttggag ggcgtgtcga gacgcttaag cggcttgccg atcagccggt
     3421 actgcgcggg atccttgagc gcgggcgagg cgggcggcgc aaacttcgaa gctgcctcgg
     3481 cgaggctgcc gtaactcaga cgcgcattgg tcgcggtatt gaccaccgtg ttgttctccg
     3541 cgcggcaggc cgacggggcg ataccccatt tggctgcggc ggcctgtacc agcatctcgc
     3601 gagcggccgc gcctgctttg cgcagcgagt cgtatgaagt gcgcacgctc tggctgccga
     3661 acacgccctg catcgggccg aattcgcggt tcacgccggg gaactcgatg cgcaccttct
     3721 tccaatcgca ttcgagttct tcggccagca gcatggatag cgacgtgacg gttccctgcc
     3781 ccatctcggc cttatgtatg aagagcgtca cggaatcgtc ggcgccgacg tgcacgaagg
     3841 cgttgagctt cgaggctccg gcggcctcag ccttttccga gaggcggaag gcgagcagca
     3901 gcccggtggc ggattgcaga aacccgcggc ggctgacatt cggcagcgcg ctcatcgggc
     3961 gcctcccttg gcgcgcaact cggcggccag atggaccgcg cggcggatcg attgatacgt
     4021 gccgcagcgg catatgttgt tgcgcatggc ctcatcgatg tcggcgtcgg tgggattggc
     4081 ggttttcgcc aggagcgcgg cggcggtcat gatctgtccg ctctggcagt acccgcattg
     4141 cggcacgtct tcagcgatcc aggcctgctg aattgggttc gttttgtcgg gcgagaggcc
     4201 ctcgatcgtc gtgacgcttt tgccggcgag cgtggaaatc ggcgtcacgc aggagcgcac
     4261 gggttggccg ttcaggtgga cggtgcaggc gccgcagagc gacatgccgc agccgaattt
     4321 ggtgccggtc atgccgagcg tgtcgcgcaa gacccataac agcggcatgg ccggtggaac
     4381 gtccaccgtc tgcgccttgc cgttcaaagt gaattttacc gctgccatcc cagccc
//
LOCUS       DATIMH010000566         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4467127, whole genome shotgun sequence.
ACCESSION   DATIMH010000566 DATIMH010000000
VERSION     DATIMH010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4467127"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="VLI90_09840"
     CDS             complement(<1..1617)
                     /locus_tag="VLI90_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14552.1"
                     /translation="MADDPLTIRGINWRETFPFTNLFRAFRVAIHPSKIVLALVALLL
                     LYVGGRILDGVWPLHDRAVPGEVAAYEAYLRGPRAGEKFDDFYRQPTRREIEDGYATQ
                     LMDLKLVTDRAAAEKAAQNGDKLGDVKGELLKQRAAAVDASNKSYTDSMTAAGKLSGD
                     ARKEAEQAAKETRDASVRSAYENAYAQYESAKRIKNHGLFDHFFQYQSSQVSNVVRGV
                     REWNWFGEEHFSERAAAANAAAANNPLAALAAGEVLSSGAGAYGSPGVIQAVVRFFTI
                     GPLWLLTQHTFYFLIFGIYFLVLWAIFGGAISRIAAVHVARDEKMSIRSALKFSISKF
                     LSFLFAPIIPLLIVLVVGLIVAIGALVGNIPFLGPIIVGALFFLALAAGFVMALVLLG
                     LVGGFNLMYPTIATEGSDSFDAISRSFSYLYARPWRLAFYTIIAVIYGALTYLFVRLF
                     IYLMLVLTHKFVSVGFLYHADSTAPLWSVMWPSPTTAARLSYDIDFLTLGVGQDIGAF
                     LVAFWVYLVISILGAFAISFYFSANTIIYYL"
     gene            complement(1787..1987)
                     /gene="yacG"
                     /locus_tag="VLI90_09845"
     CDS             complement(1787..1987)
                     /gene="yacG"
                     /locus_tag="VLI90_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HSV14553.1"
                     /translation="MLCPICKKPVEEPRPGQPPPRCFPFCSDRCKLIDLGRWLGGKYQ
                     LPVEISPDEDDKPAAPPPPPRP"
     gene            complement(2025..>2427)
                     /locus_tag="VLI90_09850"
     CDS             complement(2025..>2427)
                     /locus_tag="VLI90_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HSV14554.1"
                     /translation="EMPTYEYECEACGHKFERFQSIKAEPVKKCPECGKNKVKRLIGI
                     GAGMIFKGSGFYITDYRDKSYTEKAKAESGGGGATDAAKSSSEGKSDAKPATSDASSK
                     PAETKAAGDSNSSSAPAPKSEPRASKSAKKK"
BASE COUNT          452 a          708 c          821 g          446 t
ORIGIN      
        1 caggtaatag atgatcgtgt tcgctgagaa gtaaaagctg atcgcgaacg cgcccaggat
       61 cgagatgacg aggtacaccc agaaggcgac gaggaacgcg ccgatgtcct gcccgacgcc
      121 gagcgtgagg aagtcgatgt cgtaactgag tcgtgccgct gtcgtcgggc tgggccacat
      181 gaccgaccag agcggcgcgg tggagtcggc gtggtacagg aagccaacgc tgacgaactt
      241 atgcgtcagc acgagcatca ggtagatgaa caggcggacg aacaggtagg tcagcgcgcc
      301 gtaaatgacg gcgatgatcg tgtaaaacgc cagccgccag gggcgggcat acagatagct
      361 gaagctgcgg ctgatggcgt cgaagctgtc ggagccttcg gtcgcgatcg tcgggtacat
      421 caggttgaac ccgccgacga gtccgagcag caccagcgcc atcacgaacc cggcggccag
      481 cgccaggaag aagagcgcgc cgacgatgat cggcccgagg aacgggatgt ttccgacgag
      541 cgcgccgatg gccacgatca ggccgaccac aagcacgatc aacagcggga tgatcggggc
      601 gaacaggaag ctgaggaact tgctgatcga aaacttcaag gccgagcgga tgctcatctt
      661 ctcatcgcgc gcgacatgca cggcggcgat gcggctgatg gcgccgccga agatcgccca
      721 gagcacgaga aaatagatgc cgaagatcag gaagtagaac gtgtgctggg tgaggagcca
      781 cagcgggccg atcgtgaaga agcgcacgac cgcctggatg acgccggggg aaccgtaggc
      841 gccggcgccg gacgagagga cttcgcccgc ggcaagggcg gcgagcgggt tgttcgcggc
      901 ggcggcgttg gcggcggcgg cgcgctcgct gaagtgctct tcgccgaacc agttccactc
      961 gcgcacgccg cgcacaacgt tacttacctg gctcgactgg tattggaaaa agtggtcgaa
     1021 cagaccgtga ttcttgattc gcttggctga ttcatactga gcataagcgt tctcgtacgc
     1081 gcttcgaacg ctggcgtcgc gcgtttcctt cgcggcctgc tcggcttcct tgcgcgcatc
     1141 gccagacagc ttgccggcgg cggtcatcga atcggtgtaa gacttgttcg aggcatcgac
     1201 ggcggcggct cgctgcttca gcagttcgcc tttgacgtcg ccaagcttgt cgccattctg
     1261 cgccgccttc tcagcggcgg ccctgtccgt caccagcttc aaatccatca gctgcgtggc
     1321 atagccgtct tcgatttcgc gccgcgtggg ctgacgatag aagtcgtcga atttctcgcc
     1381 cgcgcgcggg ccgcgcaggt aggcttcata cgccgccact tcgccgggca cggcgcgatc
     1441 gtgcagcggc cagacgccgt caaggatgcg accgccgacg tacagcagca gcagcgcgac
     1501 cagcgcgagc acgatcttgg aaggatggat ggcaacgcgg aaggcgcgaa agaggttggt
     1561 gaacgggaac gtttcgcgcc aattgatgcc acggatcgtc agcgggtcat ccgccatgag
     1621 tggtgctctc cttccgagcg atgccgagtc ggtgctgtgc gtgcgcgcgc gatcagtcgc
     1681 ggtgcgcgcg ggcattataa ggagaatgga ttgtgtcaca actcagctac ccggggggcg
     1741 cgaatcgcca agcacaggcg cgaccccatg ctacgaattg cgccattcag ggacgcggcg
     1801 gcggtggtgg cgccgccggt ttatcgtcct cgtccggcga gatctcgacg gggagttgat
     1861 acttgccacc gagccagcgg cccaggtcga tcagcttgca gcggtcgctg cagaacggga
     1921 aacagcgcgg cgggggttgg ccggggcggg gttcttcaac gggctttttg cagatggggc
     1981 agagcatatc cgtccgccca ttttagcgcg cttcgtcgaa gcggtcactt cttcttcgcc
     2041 gacttcgatg ccctgggttc gctcttgggg gcgggcgcgg aggatgagtt ggaatccccc
     2101 gcggccttgg tctcggcggg tttcgatgaa gcgtcgctcg tcgcgggctt ggcgtcggac
     2161 ttgccttcgg atgaagactt cgccgcgtcc gtcgcgccgc caccgccgct ttcggccttg
     2221 gctttttcgg tgtaggactt atcgcgatag tcggtgatat agaagccgct gcccttgaag
     2281 atcatgccgg cgccgatgcc gatcaggcgc ttgaccttgt tcttcccgca ctcgggacat
     2341 ttcttgaccg gttcggcctt gatggactgg aagcgctcga acttgtgtcc gcacgcttca
     2401 cattcgtatt cgtaggtagg catttcg
//
LOCUS       DATIMH010000567         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4177812, whole genome shotgun sequence.
ACCESSION   DATIMH010000567 DATIMH010000000
VERSION     DATIMH010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4177812"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VLI90_09855"
     CDS             complement(<1..714)
                     /locus_tag="VLI90_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14555.1"
                     /translation="MADAAGFTQLLRSRGVAVPPGVDVRPPRVALYFGEALGDNLLCT
                     AVLHELRRRGQRDLWMMTGYSELFHANADVDALVPATEKWFELAVTQFGGRFIVPAYT
                     RHDWQNDAGDPPPPAHVIAMMCYLAGVTGPVALRPYLNLEPAELSFGRFGPRQIAIQS
                     TGLGAAMSMRNKEWLPERFQAVVDALKNDFQFVQLGAAGDPPVDGAIDLRGQTTLRQS
                     AAVLSHSLMFVGLVGFLMHL"
     gene            713..>2290
                     /locus_tag="VLI90_09860"
     CDS             713..>2290
                     /locus_tag="VLI90_09860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HSV14556.1"
                     /translation="MSANFLGWCEGLSSGIDRATTPPTVRRESSLNRLISQSDPWDLA
                     VIGGGATGVGIAVDAASRGYSCCLLEQSDFGKGTSSRSTKLIHGGVRYLQQGNISLVM
                     EALKERGILRRNAPHLVHDLAFVVPNYSWWEAPFYGIGMKVYDVLAGRHGFGKSRVLS
                     RDKVLEHIPTLEQAGLRGGVLYYDGQFDDARLLIDLAHTADAQGAVLLNYAKVVRLNH
                     DSEGFVNGLTFRDIESGNDHDVQARCVINATGAFSDEVRRMDEPDSPPMIAPSQGVHL
                     VLDRSFLPGDAAIMVPRTSDGRVMFAIPWLGHTLLGTTDTPITDVSLDPTPLAQEVDF
                     ILETAAKYLARDPSRSDVLSAFAGIRPLVKNTEASSTAALSRDHTIHVAKSGLLTIAG
                     GKWTTYRRMAKDCVDHAATLAQLEERPCLTRDLQIHGADGEGDAELKKLIEGDPTLAD
                     ALSAQLSITAAHVVLSARSEMARTVDDVLARRTRALFLNAKAAVDMAPIVARLLGREL
                     SRDQAWIDRQIGDFTHLA"
BASE COUNT          422 a          768 c          713 g          387 t
ORIGIN      
        1 caggtgcatc agaaaaccaa cgaggccgac gaacatcagc gaatggctca gcaccgccgc
       61 gctctggcga agtgtcgtct ggccgcgaag gtcgatcgcg ccatccaccg gcggatcgcc
      121 cgcggctcca agctgtacga attggaagtc gttcttcagc gcatccacca ccgcctgaaa
      181 ccgttccggc agccactcct tgtttcgcat cgacatcgcc gccccgaggc ccgtgctttg
      241 gatggcgatc tgtctgggtc caaagcggcc gaagctcaac tccgcgggct cgaggttcag
      301 atacggccgc agcgccaccg gcccggtgac gcccgcgaga tagcacatca tcgcgatgac
      361 gtgcgccggc ggcggtggat cgccggcgtc attctgccag tcgtgcctcg tatacgctgg
      421 gacaataaag cgcccgccga attgtgtgac cgcgagttcg aaccattttt cggtcgctgg
      481 cacgagcgca tcgacatcgg cgttcgcgtg aaacaattcg ctatagccgg tcatcatcca
      541 caggtcgcgc tgcccgcggc ggcgcagctc gtgcagcacc gcggtgcaca gcaagttgtc
      601 cccgagcgct tcgccgaaat agagcgcgac ccgcggcggg cgcacgtcca cgcccggcgg
      661 caccgccacg ccgcgcgatc gcaagagctg tgtgaaaccc gccgcgtccg ccatgtctgc
      721 caatttcctc ggctggtgtg aggggttgtc cagcgggatc gatcgtgcga caactccgcc
      781 caccgtgcga cgcgaatcat cgctgaatcg tttgatctcg caaagcgacc cgtgggacct
      841 ggccgtcatc ggcggtggcg ccaccggcgt cggcattgcc gtggacgcgg cgtcgcgcgg
      901 gtactcgtgt tgtctgctcg agcaatccga cttcggcaaa ggcacgtcga gccgatccac
      961 aaaactgatc cacggtggtg tgcgttacct gcagcagggg aacatctcgc tggtgatgga
     1021 agcgctcaag gaacgtggca tcctccgccg caacgccccg cacctggtgc acgatctggc
     1081 gttcgtcgtg cccaactaca gctggtggga agccccgttc tacggcatcg gcatgaaggt
     1141 gtacgacgtc ctcgccgggc ggcatggctt cggcaaatcg cgcgtgctgt cgcgcgataa
     1201 ggtgctcgaa cacatcccca ccctcgaaca agctggcctt cgcgggggcg tgctttacta
     1261 cgatggccaa ttcgacgatg cccgcctgtt gatcgatctc gcccacaccg ccgatgctca
     1321 gggcgcggtg ctgctgaact acgcgaaggt cgtccggctg aaccacgaca gcgaggggtt
     1381 cgtcaacggc ctgacatttc gtgatatcga aagcggaaac gatcacgacg tccaagcgcg
     1441 ctgcgtgatc aacgcgaccg gcgcgttcag cgatgaggtt cgtaggatgg acgaaccaga
     1501 tagtccgccg atgatcgccc cgagccaggg cgtccacctc gtgctggacc gttcgttcct
     1561 cccgggcgac gcggcgatca tggtgccgcg caccagcgat ggccgcgtaa tgttcgccat
     1621 tccctggctc ggccacacgc tgctgggcac gaccgacacg ccgatcaccg atgtgagcct
     1681 cgatccgaca ccgctggcgc aggaagtgga tttcatcctg gagacggcgg cgaaatatct
     1741 ggcgcgcgac ccatcacgca gcgatgtgct cagcgccttc gccggcattc ggccgctggt
     1801 gaagaacacg gaggcgtcga gcaccgccgc cctgtcgcgc gatcacacca ttcacgtcgc
     1861 caagtccggc ttgctcacga tcgccggtgg gaagtggacg acgtatcgcc ggatggccaa
     1921 ggactgcgtc gatcacgcgg ccacgctggc gcagctggaa gagcggccgt gtctcacgcg
     1981 cgacctgcaa atccacggcg cggacggcga aggcgacgct gaattgaaga agttgatcga
     2041 aggagatccg acgctcgccg acgcgctaag cgcccaactt tcgatcacag ccgcgcacgt
     2101 tgtactgtca gcgcgatcgg agatggcccg caccgtcgat gacgttctcg cccggcgaac
     2161 gcgcgcgctg tttctcaacg ccaaagccgc cgtcgacatg gcccccatcg tcgcgcgcct
     2221 gctcgggcgc gagctgtcgc gcgatcaagc atggatcgac cgacaaatcg gcgacttcac
     2281 ccacttagcc
//
LOCUS       DATIMH010000568         4670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3312634, whole genome shotgun sequence.
ACCESSION   DATIMH010000568 DATIMH010000000
VERSION     DATIMH010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3312634"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..1134
                     /locus_tag="VLI90_09865"
     CDS             439..1134
                     /locus_tag="VLI90_09865"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSV14557.1"
                     /translation="MLCITGSSFGQIKLAASPHNLNNWAGAKVDKQQVCLPCHTPHGS
                     ALNSEGGTGELLWNHNTNWGTVYTMYTSPGKHSTRYSGTSSANPVLDSTSRLCLSCHD
                     GAIAIDSYGASGSNTVSTLTIDKLDTGSAKLGTDLSTSHPVGISYPGLSADGKTFTAS
                     SGSGYIDPTTASWGRTVTTGSALGGGISFATVSGKANVIGCGTCHTPHTYTYNFVKVD
                     NTGSQLCFKCHDK"
     gene            1321..2646
                     /locus_tag="VLI90_09870"
     CDS             1321..2646
                     /locus_tag="VLI90_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14558.1"
                     /translation="MTAQSAQSDQSRTSRPTKAARRHPRRRGFTFAAIALFATATAFT
                     GGCASNKPALQATSDRTYACWPPFPQEPRVVYLRSFSSTLDLEQQKTKLDEIMYGKDP
                     QQESMIQHPYGLDMYKGLIYVCDTKSGIVIIDLHKKQMRLMGVGGAVQVTKPVDIAIA
                     PDGRKYVADSQRESVYVFDANDVFQAQIGHAGLKPVSVAVHDNELFVSDFKSNRIEVF
                     DRNTGKSLRFIGGPGQEKGKLVDPIGVDVDKEGNVYVDDIVNCRIQKFTPDGKKVTLS
                     IGALGDRPGTFTRPKHLAVDQEGIIYVVDAAFQNVQMFNQQGKGLLFFGSAGTHPGSM
                     DMPAGICVHDGDLDLFASDIPPAFDAQRLVLVTNQFGDDRVSVYALGHLKAGHSVNEL
                     AGSKGLVPVGTSSEPTQGPGFPLDVAQPTHSGAATTMPAAAAPSPAVTP"
     gene            2843..3511
                     /locus_tag="VLI90_09875"
     CDS             2843..3511
                     /locus_tag="VLI90_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSV14559.1"
                     /translation="MALAFAALVIGCTPQRNYRVLSFFFDGVPDPNAPKTTPSLALMS
                     GDERKAMKLAVVHKPYAEGNCRSCHASLDNVKPDSGSQDVLSATGKTLKLVAMDSSLC
                     LSCHKDKPGEFKVMHGPVASAACMWCHEPHQSENAALLKSRSADLCLQCHERQLLTTE
                     AKEHQNEARACLDCHFGHGGPDTNLLRPKPLAPLPPPTADRATTQPSVALAPAAPANA
                     EVAP"
     gene            3550..>4670
                     /locus_tag="VLI90_09880"
     CDS             3550..>4670
                     /locus_tag="VLI90_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14560.1"
                     /translation="MAAWAAIGAAVAASGSAADAQVQPLVNSGGPIVLGEQHEKPDAV
                     HFDPVTGSVNLLARYDANTSDQGSTHERAAESLFEETATVESGGYVYHPNIFSFHVGV
                     TGGLSQQLFDSSNASPQTDKTGFDSLYGWDISGTFLRNEYAPLTLYTRRFEQFIQRDF
                     SPSERSTSTTYGANLDIRSRIVPTTFQVYHSDDTLTSLAPGVGYDLTQNVAQWHSEAD
                     PTANQKLIWDASFNNTYQAPNIGPSFTINSGDANLSHDISFGKDKASSLLSSINYTNQ
                     TGDIAFNRLRWSESLRLKHSEQFLTQYNYAFDRESFQGSEQTNHRLNAGFVHHLFESL
                     TTSGNVGGALTDLGSGGNIEQFFSSLGFDYRKNVPFGVLL"
BASE COUNT          980 a         1578 c         1362 g          750 t
ORIGIN      
        1 gggcggggcg cgaggcgcgc gatgccggcc acacggcgat tgagaacaac ttccatgccg
       61 cagaaggcga aatgacggcg agggcgcaca gccacgctta acccacccgc ccggcaccgt
      121 ttacccccac cggcgactcg cgagccaata aacagactgt cgaatctttt ttcttagtcg
      181 cgggaacgcc tcttcaacgc aactcccgtc gcaaagagcg ggcgtcggtc gccaaccccc
      241 gcccggaggg catttcggct atccgaactc aataaaagga ctggcatgtc tcttgcccat
      301 gtcaccacag cgtctcgatc ggacggaacg ctgaccgggg tggcgggacc ggtaaccaag
      361 aaactcccgc attcttcgga ttcggagcga cctatgagat tctcaaacaa actagcggcc
      421 ggcgccgcac tcgctgtgat gctctgtatc acagggagca gttttggcca gatcaagctc
      481 gccgcaagcc cgcacaacct aaacaactgg gcgggcgcca aggtggacaa gcagcaagta
      541 tgcctgcctt gccacacccc gcacggttca gcgttgaaca gcgaaggtgg cacgggtgag
      601 ttgctgtgga accacaacac caactggggc acggtctaca cgatgtatac cagccccggc
      661 aaacactcga cgcgctacag cggcaccagc agcgcgaacc ccgtgctgga ctcgaccagc
      721 cggttgtgcc tctcctgcca tgacggtgcc atcgccatcg attcgtatgg tgcgagcggc
      781 tcgaacaccg tcagcacgct gacgatcgac aagctggaca ccggttcggc caagctgggc
      841 acggacctga gcaccagcca cccggtcggc atcagctacc cgggcctgtc ggcggacggc
      901 aagacgttca ccgcctcctc gggcagcggt tacatcgacc cgaccaccgc cagctggggc
      961 cggaccgtga ccaccggtag cgccctgggt ggtggcatca gcttcgccac cgtcagcggt
     1021 aaggccaacg tgatcggctg cggcacctgc cacacgccac acacctacac ctacaacttc
     1081 gtgaaggtgg acaacaccgg cagccagttg tgcttcaagt gccacgacaa atgaagagtt
     1141 gaccacgtgt gtcaacagcc acccggcggg ccggtcccga agaaccggcc acgccagggg
     1201 cctcaagcac cggttccgca ccccctgcgg cagtgttccg tccgaccgag attgtatgca
     1261 acgatgaaga aaaaggaaca acttcaaacg acgacccaat ctgttgatca ggtgaccgcc
     1321 atgaccgccc aatccgccca atctgaccaa agccgtacgt cccgaccgac caaagccgca
     1381 cgacgccacc cccgccgtcg cggtttcacc ttcgcggcga tcgcgctgtt tgccaccgcc
     1441 accgccttca ccggcggttg cgccagcaac aaaccggccc tgcaggccac cagcgaccgc
     1501 acctacgcct gctggccgcc attcccgcag gaaccgcgtg tcgtgtatct gcgaagcttc
     1561 agcagcaccc tcgatctcga acagcagaaa accaagctcg atgagatcat gtacggcaag
     1621 gacccgcagc aggagtccat gatccagcac ccgtacggcc tggatatgta caaggggctg
     1681 atctacgtct gcgacaccaa gagcggcatc gtcatcatcg acctgcacaa aaagcagatg
     1741 cggttgatgg gcgtcggcgg cgcggtgcag gtgaccaagc ctgtcgatat cgcgatcgcg
     1801 cccgacggcc gcaagtacgt cgccgacagc cagcgtgaat cggtctatgt cttcgacgcc
     1861 aacgatgtct ttcaggcgca gatcggccac gcgggtctga agcccgtgag cgtcgcggtg
     1921 catgacaacg agctgttcgt cagcgacttc aagtcgaacc ggatcgaagt cttcgaccgc
     1981 aacacaggca agtccctgcg gttcatcggc gggccaggcc aggaaaaggg caagctggtc
     2041 gatccgatcg gcgtggatgt ggacaaggag ggcaacgtct acgttgacga catcgtcaac
     2101 tgccgcatcc aaaagtttac gcccgacggg aagaaggtca cgctgtcgat cggcgcactg
     2161 ggcgatcgcc ccggcacgtt tacgcggccg aaacatctgg cggtcgatca ggagggcatc
     2221 atctacgtcg tcgatgccgc cttccagaac gtgcagatgt tcaaccagca aggcaaagga
     2281 ctgctgttct tcggctcggc cggcacgcac cccggctcga tggacatgcc cgcgggcatc
     2341 tgcgtgcacg acggggatct cgatctgttc gccagcgaca tcccccccgc cttcgacgcg
     2401 cagcggctgg tgctcgtgac caatcagttc ggcgacgacc gcgtgtcggt ctacgcgctg
     2461 ggacacctca aagccggaca cagcgtgaac gagttagccg gcagcaaagg gctggtgccg
     2521 gtgggaacat cgtcggaacc gacgcaggga ccgggcttcc cgctggacgt tgcgcagccg
     2581 acccattccg gcgcggcgac aacgatgccg gcggccgcag caccttcacc ggccgtgacg
     2641 ccatgaccga ctaacgcctg cccctgcgtg tgtgcccacg acggaacgac ttacgcgcac
     2701 acacgcacgg agatccgggc gcgcggacaa catcacacga tcgatatgaa acggcggcga
     2761 gatgtcagcg ctggtagttc atcctcaagg gcgggcgcgc ggatactcca aatggatatc
     2821 gcgatcgctg gcatttgcgc tgatggcgct cgccttcgct gccctggtga tcggctgcac
     2881 gccgcagcgc aactacaggg tgctcagctt cttcttcgac ggcgtgcccg atcccaacgc
     2941 gcctaagacc acaccgtcac tggcgttgat gagcggcgac gagcgaaagg caatgaagct
     3001 cgccgtcgtc cacaagccct atgccgaggg caattgccgc tcgtgccatg cgtcactgga
     3061 caacgttaaa ccggacagcg ggtcgcagga tgtgctgtcg gcgacgggca agacgctgaa
     3121 gctcgttgcg atggactcga gcctgtgcct cagctgccac aaggacaagc ccggcgagtt
     3181 caaggtgatg cacggcccgg tggcgtcggc cgcgtgcatg tggtgccacg agccgcacca
     3241 gtccgaaaac gccgccctgc tcaagagccg cagcgccgac ctgtgcctgc aatgccacga
     3301 gcggcagctg ctgaccaccg aagccaagga acaccagaac gaagcgcggg cgtgcctgga
     3361 ctgccacttc ggacatggtg gtccggatac aaatctgctg cgaccgaagc cgctggcgcc
     3421 gctgccgccg ccgacggccg atcgcgcgac gacgcaaccg tccgtcgcac ttgccccggc
     3481 cgcgccagcc aatgccgagg tcgcgccatg acgaccatag cgcgattctc ggggcgtttg
     3541 gcaattgcaa tggcagcgtg ggcggcaatt ggtgccgccg tcgcggccag cggcagcgct
     3601 gccgacgccc aggtgcagcc gctggtgaat tccggaggac cgattgtgct tggcgaacag
     3661 cacgaaaagc ccgatgccgt tcacttcgat ccggtcaccg gatcggtcaa cttgctcgcg
     3721 cgctacgacg ccaacacctc cgaccagggc agcacgcatg agcgcgccgc cgaatcgctc
     3781 ttcgaagaaa ccgccaccgt ggagtccggc ggttacgtct atcaccccaa catcttctcg
     3841 ttccacgtcg gcgtgaccgg cggattgtcc caacagctgt tcgacagcag caacgccagc
     3901 ccccaaaccg acaagacggg cttcgactcc ctttacggct gggacatcag cggcacgttt
     3961 ctgcgtaacg agtacgcgcc gttgacgctg tacacgcgcc ggttcgagca gttcatccag
     4021 cgcgatttct cgccgtccga acgatcgact tccaccacct acggggcaaa cctcgacatc
     4081 cggtcgcgca ttgtgccgac cacttttcag gtttatcact ccgacgacac cctcacctcg
     4141 ctcgcgcccg gcgttggtta tgacctgacg cagaacgtcg cccagtggca cagcgaggcc
     4201 gatccgaccg ccaatcagaa gctgatctgg gacgcgagct tcaacaatac atatcaggcg
     4261 ccgaacattg gccccagttt caccatcaac tccggcgatg cgaacctctc gcatgacatc
     4321 agcttcggca aggacaaggc cagcagcctg ctgtccagca tcaactacac gaatcagacc
     4381 ggcgacatcg cgttcaatcg tttgcgctgg agcgaatcgc ttcgcctgaa gcattccgaa
     4441 cagttcctga cgcaatacaa ctacgcgttc gatcgcgagt cgttccaagg ctccgagcag
     4501 accaaccacc gcctcaacgc cggcttcgtc catcacctgt tcgaaagcct caccaccagc
     4561 ggcaacgtcg gcggggcgct gacggacttg ggtagcggcg gaaacatcga gcaattcttc
     4621 tccagcctcg ggttcgacta tcgcaagaac gtcccgttcg gcgtgctgct
//
LOCUS       DATIMH010000569         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5049457, whole genome shotgun sequence.
ACCESSION   DATIMH010000569 DATIMH010000000
VERSION     DATIMH010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5049457"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VLI90_09885"
     CDS             <1..553
                     /locus_tag="VLI90_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14561.1"
                     /translation="PYHNYLFQPRELPQVCFIECKASRADFLRDKSDDGQMMFCLMER
                     KRNLTRGRSKRTRFTRLRQSVGLGKFEACLTQPMANLHYVLAPAGIVQKKDLPPRWGL
                     LSFGEGGINVVVRSDWQESARTEFVESAIARTLTGDIYRADDRAINSVNREIFAQQQQ
                     LATRIRNVRPQVVFAPMNDAAAS"
     gene            736..2520
                     /locus_tag="VLI90_09890"
     CDS             736..2520
                     /locus_tag="VLI90_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14562.1"
                     /translation="MNSARRLASISAAIAVAAAAAPTARAVNAVYNPGFVSGSNFTGG
                     NVEVQGSSGRLDLPVTFQTAPVDFFLSFSVAGATANDTTAYTLLTPLQPSSNSSLLME
                     DPHTGAYLGTVALNARFSDIAYTNGQLYGIGSSSTSLQVSTIAANGATQVVLNQTMPG
                     SGGTWRLSGAADGSALLAARAFFSSSNVPGFVVNPTSLAVSQITLSGTASTQLDDNVI
                     NQPANAVASIGGTQQVLTAFPGGGALGNVPGNARYGTAFISNTVSDEFKYNYSPSMAA
                     VMQTTWNVTASPLVGTTDGLTRQPQVVTVSGPISISNYVTPDGTTLSNVAVTNQIAQY
                     MGPQFGHTPFVTSAQNVPASDLSNRNVGQGSASLDLSKAERGLYDVHVTSSATADYSY
                     ASTVDSNRFVQTGTLGSIEFAYDPISNNLVGNGDAYLNGAGWQGMSGSLSSFQSQWVN
                     SDPTNHCFVLIPQASYPSSMRQFLQQTTPDGPMSLSFTYDLRTLPTGGTDEIQAFLNG
                     VLVADLMPSNSSLQTFQTEIDDPTLENASSVVLMFRTTSTAASGNFIDIGNISLTSIP
                     EPTTGLLMMIPAAFMLRRRRPTSLTQSL"
     gene            complement(2677..3108)
                     /locus_tag="VLI90_09895"
     CDS             complement(2677..3108)
                     /locus_tag="VLI90_09895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSV14563.1"
                     /translation="MPKLRRILTAVAVASLSSVLIAADAPTSGEKKTTPSGLQIIEQG
                     QDDTTAAAGDKVWVWYTGKLQDGTVFDSSANHPDQPFIFSLGQHQVIAGWDEGIAGMK
                     VGEKRQLIIPPNLGYGSRAIDRIPANSTLIFDVKLMGVQKP"
BASE COUNT          657 a         1065 c          940 g          629 t
ORIGIN      
        1 gccctatcac aactacctgt ttcagccgcg cgaattgccg caggtctgct tcatcgaatg
       61 caaggcgtcg cgcgccgact tcctgcgcga caagagcgac gacgggcaga tgatgttctg
      121 cctgatggaa cgcaagcgca acctgacccg cggccgcagc aaacgcacgc gtttcacgcg
      181 gcttcgtcaa tcggtcggcc tgggcaaatt cgaagcctgc ctcacgcagc ccatggccaa
      241 cctgcactac gtgctggccc ccgccggcat cgtgcagaag aaagacctgc cgccgcggtg
      301 gggactgctg tcgttcggcg aaggcgggat caacgtcgtg gtgcgatcgg actggcagga
      361 aagcgcgcga acggagttcg tcgaatcggc catcgcccgc acgttgaccg gcgacatcta
      421 tcgagccgac gatcgcgcga tcaactccgt caatcgagag atcttcgccc agcagcagca
      481 actcgccacg cgcattcgta acgtgcgccc gcaggtcgtc ttcgcgccga tgaacgacgc
      541 cgccgcttca tagagccatc ggcgactgtt gttccggcga tctcaaaaat aaattccgat
      601 ttccccgcca acaacccaac cacctgcgtt gtctaactga cacgaatgcg gccgaccgcc
      661 gtgccgcgcg aagttgtttt tcattccctc cgggtggggg cattttccaa tcgtgccaga
      721 aaagggagag catccatgaa ttcagcaaga aggcttgctt cgatttccgc tgcgattgcc
      781 gtcgccgccg ccgccgcgcc caccgctcgg gcggtaaatg cggtttacaa tccagggttc
      841 gtcagcggtt ccaacttcac cggcgggaat gtcgaagtgc aggggtccag cgggcgattg
      901 gatttgccgg tcacgtttca gacggcgccg gtcgattttt ttctgtcctt cagcgttgcc
      961 ggtgcaaccg cgaatgacac gacggcttac acgctgctga cccccttgca accatcgagc
     1021 aattcttcgc tgttgatgga ggatccgcat accggcgcgt atctcggcac cgtcgcgctc
     1081 aacgcgcggt tcagtgatat cgcgtacacc aatggccagc tttacggaat cggaagtagt
     1141 tcgacatcgc tgcaggtttc gacgatcgcc gcgaacggcg cgacgcaggt ggtgctgaac
     1201 cagacgatgc ccggttccgg cggcacctgg cgcctgagcg gcgccgccga cggcagtgcg
     1261 ctgctggccg ctcgcgcgtt cttctcgtcg agcaacgtgc cgggcttcgt cgtcaatcca
     1321 acgtcgctgg cggtctcgca aatcacgttg agcgggactg cttcgactca actcgacgac
     1381 aacgtcatca accaacccgc caatgcagtg gcatcgattg gaggcactca gcaagtcctg
     1441 accgcgttcc caggcggcgg tgcgcttggg aacgtgccgg gcaacgcgcg atacggaacc
     1501 gccttcatca gcaacaccgt cagcgacgaa ttcaaataca actattcacc atcgatggcc
     1561 gcggtgatgc agaccacctg gaacgtcacc gcatcaccgt tggtgggcac gacggatggg
     1621 ctgacgcgtc agccgcaggt ggtgaccgtc agcggtccga tcagcatcag caactatgtc
     1681 acgcccgacg gcacgacgct cagcaacgtc gcggtgacga atcagatcgc gcaatacatg
     1741 gggccacagt tcggccacac gccgttcgtc acgtctgccc agaacgtgcc cgccagtgat
     1801 ttgtcaaacc gcaacgtggg acagggcagc gcatcgctgg atttgtccaa ggccgagcgc
     1861 gggctttacg acgttcacgt gacttctagc gcgacggctg actattccta cgcgtccacc
     1921 gtcgacagca atcgcttcgt tcaaacgggc accctgggca gcatcgaatt cgcctatgac
     1981 ccgatctcca acaacctcgt gggcaacggc gacgcgtatt tgaacggcgc cggctggcaa
     2041 ggcatgtccg ggtcgctgtc gtcattccaa agccagtggg tcaactccga ccccaccaac
     2101 cactgtttcg tccttatccc gcaggcgagt tacccgtcat cgatgcgcca gttccttcag
     2161 cagacgacgc cggatggacc gatgtcgctt tcgttcacgt acgatctacg cacccttccc
     2221 accggcggca ccgacgagat ccaggcgttc ttgaacggcg tgctcgtggc cgacctcatg
     2281 ccgagcaact cgtcgttgca gacgtttcaa acggagatcg atgatccgac gctggagaat
     2341 gccagcagtg tagtcctgat gttccgcacg acatccaccg ccgcttcagg gaatttcatt
     2401 gatatcggca acatctcgct gaccagtatt ccagaaccga cgacgggcct gctgatgatg
     2461 atccccgccg cattcatgct gcgccggcgg cgacccacgt cgcttactca gagcctgtaa
     2521 aacgacgcgc aggttattta acacagaggc acggacacac tgagaaatgc cgcaacgaat
     2581 ctgcggtctt tctccttcgt gtctccgtgc ccctgtggaa acggagtgtt attggtagct
     2641 agcaatccca ggattgttga gccgcgcctg cacgccctac ggcttctgca cacccatcag
     2701 cttcacgtcg aaaatcagcg tggaattcgc cggaatgcga tcgatcgccc ggctgccgta
     2761 gcccaggttt ggcgggatga tcagctgccg cttctcgccg actttcatgc cggcgatgcc
     2821 ttcgtcccac ccggcgatca cctggtgctg cccgagcgaa aagatgaacg gctgatcggg
     2881 atggttcgca gacgaatcga acaccgtgcc atcctgcagc ttgccggtgt accacaccca
     2941 aaccttatca cccgcggcgg cggtcgtgtc gtcctgcccc tgttcgatga tctgcaaacc
     3001 cgacggcgtg gtcttcttct cccccgaagt cggcgcatcc gcggcgatca aaacgctcga
     3061 caacgaggcg acggcaacgg cggtgagaat tctgcggagc ttgggcatgt ggttcctcaa
     3121 tggttggcaa tcccgagcgg cgcccatcga catcgacagc acgcgcccga caagtcggct
     3181 gggatgatag tacgggctgt cgaaggcgac aacgaattcc gaccggataa ggaagaccgt
     3241 ttcacccccc acgtcgggtt cggagcgtac tcgcgacgac ccggatgctt c
//
LOCUS       DATIMH010000570         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5624836, whole genome shotgun sequence.
ACCESSION   DATIMH010000570 DATIMH010000000
VERSION     DATIMH010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5624836"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1367
                     /locus_tag="VLI90_09900"
     CDS             join(232..466,466..1367)
                     /locus_tag="VLI90_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611297.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HSV14564.1"
                     /translation="MPRRYSREFKLSAVKLVTEQGYSVVDAAKSLGIDASNIRFWLQK
                     FASDEAATPKGDGVLQAEIRKLRKENAQLIMERENFKKSGGILRQGAAVKFAFIKTHR
                     RCWPVQVICRVLQVKRSGFYAWLNRKPSARQLRQEELLAKIRVAHAENRELYGSPRVH
                     RALLVDGEMVSRNTVAKLMRQAKIRAKSRPRYVPRTTDSAHTKPVADNVLDRDFTASA
                     PDRKWVADITYVPTDQGWLYVAAVLDCFSRKVVGWAMADHMETDLVAGALKMALEHRQ
                     PDGKNLLHHSDRGVQYASDDYMHLLRSHKIAVSMSGRGDCYDNAMMESFWATLKTELV
                     HHEQYATREQAKQSIFEYVEVFYNRKRLHSSLGYVSPESFEAAA"
BASE COUNT          311 a          441 c          502 g          263 t
ORIGIN      
        1 gacgatcgaa gcactcgccg aacggttcga tctccccgag ctgcgtgagg ccgtcgcgtt
       61 gcgctctggc gctccgcatc agggggcatg aatgcgggaa ggaaagagtg gtgaggagag
      121 ggtccgggag aggagcggcg agaaaaactg ggggcccgtc tggttggact tccccacgtt
      181 tggtgggcgc gctggcggga cggcatcatg agctgtccca aggagcgttt tatgccgcgt
      241 cgttattcac gtgagttcaa gctttcggcc gtcaagctgg tgaccgagca agggtacagc
      301 gttgtcgatg ccgccaagag tctggggatc gatgccagca acatccggtt ctggctgcag
      361 aagttcgcca gcgacgaagc cgccacgccc aagggtgacg gggtgctgca ggcggagatc
      421 cgcaagctgc gcaaggagaa cgcgcagctg atcatggagc gggagatttt aaaaaaagcg
      481 gcggcattct tcgccaggga gcagccgtga agttcgcgtt catcaaaacg caccggcgct
      541 gttggccggt tcaggtgatc tgccgcgtgc tgcaggtgaa gcgcagcggg ttctacgcgt
      601 ggctgaaccg caagccgtcg gcgcggcagc ttcgtcagga agagctgctg gccaagatcc
      661 gggtggcgca cgccgagaac cgcgagctgt acggcagtcc gcgcgtgcac cgcgcgctgc
      721 tcgtcgacgg cgaaatggtc agccgcaaca cggtcgcgaa gttgatgcga caagcgaaaa
      781 tccgggcgaa atcgcgcccg cgctacgtcc cgcgcacgac cgacagcgcg cacacgaagc
      841 cggtggccga caacgtgctg gaccgcgact tcaccgcgtc ggcgccggac cgcaagtggg
      901 tcgccgacat cacctacgtc ccaaccgacc agggctggct gtacgtcgcg gcggtgctcg
      961 attgcttcag ccgaaaggtc gtcggctggg cgatggccga tcacatggaa acggacctgg
     1021 tcgccggcgc gctcaagatg gcgctggagc accggcaacc ggatggaaag aacctgctgc
     1081 atcacagcga ccgcggcgtg cagtacgcca gcgacgatta catgcatctg ctacgctcgc
     1141 acaagatcgc ggtgagcatg agcggccgcg gcgactgcta cgacaacgcg atgatggaga
     1201 gcttttgggc aactttgaag accgagctgg tccatcatga gcagtacgcg acgcgtgagc
     1261 aggccaaaca atcgatcttc gaatacgtgg aggtgttcta caatcgcaaa cgactgcaca
     1321 gttcgttagg ctacgtcagc cccgaatcgt tcgaggcggc agcctgacaa caacgtcgtc
     1381 gagcgcccac caaacgtggg caagtccact gtgtttctgc tctgtgatct ctgtgtgctc
     1441 tgtggttcaa ttccgttttt tgtttttcga agaacgcatg ggcttcctcg acaaagaaca
     1501 ctccatcgcc ggccccg
//
LOCUS       DATIMH010000571         3539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5338990, whole genome shotgun sequence.
ACCESSION   DATIMH010000571 DATIMH010000000
VERSION     DATIMH010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5338990"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VLI90_09905"
     CDS             <1..378
                     /locus_tag="VLI90_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14565.1"
                     /translation="LAAAIRADLLNISPELARRYDQSGGAAEQTFQQLFTRPDPAVAA
                     LLVVKDFSKLAIAEKDGSLPEEIGAVLYYAAIACAILRCGGERISELDDATLKIGLTW
                     ATAQAWLDLSLRGLFQELVDQLK"
     gene            complement(452..874)
                     /locus_tag="VLI90_09910"
     CDS             complement(452..874)
                     /locus_tag="VLI90_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSV14566.1"
                     /translation="MKTPKNIDVLFVTGFGPIVRDPTESRRFYSDTLELPLKEDADGY
                     LHTSALHGVKHFALWPLAQAARSCFGTDQWSGNLPVPQAWIEFDVADVEKATAELKSQ
                     GYQLLVATRTEPWGQIVTRLLGPEGLLVGITHTPSMRV"
     gene            complement(960..1835)
                     /locus_tag="VLI90_09915"
     CDS             complement(960..1835)
                     /locus_tag="VLI90_09915"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSV14567.1"
                     /translation="MSTKPTIALLGLGLMGTGMAARLLGAGFPLIVFNRNRERAAPLA
                     KIGARVAGNAREAAAGAEIVIAMLADDAASRAVWLGDDGALAGAGAGTLLIESSTLSV
                     AWVKELANLATARKSELLDAPVTGSRPQAAAGELNFLVGGSDAAVARARPALDVMGRQ
                     VIHLGPTGSGALVKLINNFVCGVQVAAIAEAIALIERTDLDRDKALAVLTHGAPGSPL
                     VKTLSARMTARDYTPNFLLRLMAKDLAYAQREAQDRSLELTTAAAASEVFARAIEAGL
                     SEKDFSAIVEPMRSK"
     gene            complement(1923..1996)
                     /locus_tag="VLI90_09920"
     tRNA            complement(1923..1996)
                     /locus_tag="VLI90_09920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1960..1962),aa:Arg,seq:ccg)
     gene            2426..>3539
                     /locus_tag="VLI90_09925"
     CDS             2426..>3539
                     /locus_tag="VLI90_09925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSV14568.1"
                     /translation="MGGDGKTTSAAGAGGLPAGVRLVAGGVPLSASGAQAATAAAAET
                     IAQDSTARDDAVAATSAEPRVNHTSEPGERPALIGDYELLGEIAHGGMGVVFRARQVS
                     LNRPVALKMILAGRLAGRAALERFRLEATAVARLEHPNIVPIYEVGQDGGQHFFSMRL
                     LEGGSVVEHLQRYIDDPAAAALLVEKVARAIHHAHQRGILHRDLKPGNILLDSAGEPH
                     VSDFGLAKSSEMDSGLTQSEASLGTPGFMAPEQIRGAKSITTAADIYSLGAILYQLLT
                     GVPPFSGASPMEVLRRVTDEQPPPPRSLRPRVPRDLETICLKCLRKETHLRYASAEAL
                     ADDLRRYRLGEPIEARTVGKIERLWSWCRRKPALAAA"
BASE COUNT          609 a         1092 c         1221 g          617 t
ORIGIN      
        1 ttggcggcgg cgattcgcgc ggacctgctg aacatctcgc ctgagctggc ccggcggtat
       61 gaccagagcg ggggggcggc ggagcaaacc tttcagcagc tgttcacgcg gccggacccg
      121 gcggtggcgg cgctgctggt ggtgaaggat ttttcgaagc tggcgatcgc ggagaaggac
      181 ggctcgctgc cggaagagat cggggcggtg ctgtactacg cggcgatcgc gtgcgcgatc
      241 ctgcgatgcg gtggagagcg catcagcgag ctcgatgatg cgacgctgaa gatcgggtta
      301 acctgggcga cggcgcaggc gtggctcgat ctatcgctgc gcgggttgtt tcaggagttg
      361 gtggatcaac taaaatagtt gtagcgtgcg atcgatcaat aaaagcgcac ggctctgccg
      421 tggaacgatc gcgcgccatg gccgacaaag cctacacccg catcgacggc gtgtgcgtga
      481 tgcccaccag caggccttcc ggccccagca gccgcgtgac gatctggccc cacggttccg
      541 ttcgcgtcgc aaccagcagt tggtacccct gcgacttcaa ctcagcggtg gccttttcga
      601 catccgccac gtcaaattca atccacgcct gcggcacggg aaggttgccc gaccattggt
      661 cggtgccgaa gcaagaccga gccgcctgcg ccagcggcca cagggcgaaa tgcttcacgc
      721 cgtgcagcgc gcttgtgtgc aggtagccgt cggcgtcctc cttgagcggc agttccaatg
      781 tgtcggaata aaagcggcgg ctttccgtcg gatcgcgaac gatcgggcca aagccggtga
      841 cgaataagac gtcaatgttt ttaggtgttt tcatcttggt tcgaatggcg cacgagtaat
      901 ctttttccag ttccgggcaa gatcgctagg agggacctca caccgatttg ccgttggcac
      961 tacttgctcc gcatcggttc gacgatggcg gagaaatctt tttcgctcaa tccagcctcg
     1021 atcgcacgag cgaagacctc gctcgccgcg gcggcggtcg ttagctcgag cgaccggtcc
     1081 tgcgcttcgc gctgggcgta ggccaggtcc ttggccatca accgcagcag gaagttcggc
     1141 gtgtaatcgc gggcagtcat tcgcgccgac agcgttttga ccagtggact gccgggggcg
     1201 ccgtgggtca gcacggcgag cgccttgtcg cggtcgaggt ccgtgcgttc gatgagggcg
     1261 atggcctcgg cgatcgccgc gacctgcacg ccgcagacga aattgttgat cagcttcacc
     1321 agcgcgccgc tgccggtggg accgagatgg atgacctgcc ggcccatgac atcgagcgcc
     1381 ggccgagcgc gggcgacagc ggcatcgctg ccgccgacga ggaagttcag ctcgcccgcc
     1441 gccgcctgcg ggcggctgcc ggtgaccggc gcgtcgagca attcgctctt gcgcgcagtg
     1501 gccaggtttg ccaattcctt cacccaggcg acggaaagcg tgctggattc gataagaagt
     1561 gtgccggcgc cagcgccggc gagggcgccg tcatcgccca gccagacggc gcgcgatgcc
     1621 gcgtcatcgg cgagcatggc gatgacgatc tccgcgccgg ccgcggcttc gcgcgcgttc
     1681 ccggcgacgc gcgcgccgat cttcgccaat ggcgcagcgc gctctcgatt gcgattgaac
     1741 acgatcagcg ggaaccccgc ccccagcagc cgcgcggcca tgccggttcc catcaagccc
     1801 aatcccaaaa gcgcgatcgt tggtttggtt gacatgcaag gcaacgttag gccatcggcc
     1861 acacgctggc tagtttcggc gtcgagatgt tgaggaggcg acgcgggcaa ggggcgggaa
     1921 agcgttcccg ataggggtcg aacctataac cttcggcttc ggaggccgac gctctatcca
     1981 attgagctac gggaacattg gttggcgaac gcctgggatg atagagcgct gcggcggacg
     2041 tgtaaacccg ccccgacgcc ggggtctgag gtactcccag agaaatcgca tgtagctcct
     2101 tccccctcgc ccggtactgc tgaccgcagg tctccgggag agggcggggg tgtgggtgat
     2161 tttcattttc gaccgccgct ggcattccga atcaccctaa ccctctcccg gcgacctgcg
     2221 gtcagcagta ccgggagagg ggaccagagc gcgcgtttac tacatgcgat tgccctggag
     2281 gtacttcgaa gtcccgggtc ttgggcgtac ctcagaccct ggcgtcgggg ttattgccga
     2341 atcctgagcc acattgccaa gcattgcgcg cgagcgcgcg aatcgtatct tccacactat
     2401 actggtaccc ggcgcggtga aacctatggg cggcgatggc aagacaactt ccgcagccgg
     2461 cgcgggcggg ttgccagccg gcgttcgcct cgttgcgggc ggggtgccgc tgtcggcgtc
     2521 gggcgctcaa gccgccacgg cggcggcggc ggagacgatt gcgcaggact cgacggcgcg
     2581 cgacgatgct gtggccgcga ccagcgctga gccgcgcgtg aaccacacat ccgagccggg
     2641 cgagcggccg gccttgatcg gggactatga actgctcggt gagatcgccc acggcgggat
     2701 gggcgtggtc tttcgcgcgc ggcaggtcag cctgaatcgc ccggtcgcgc tgaaaatgat
     2761 cctcgccggt cgtcttgcgg ggcgcgcagc gctggaacgg tttcgattgg aagcgacggc
     2821 tgtcgcgcgc ttggagcatc ccaacatcgt gccgatctac gaagtcggcc aggatggcgg
     2881 ccagcatttc ttcagcatgc ggctgctgga aggcggcagc gttgtcgagc atctgcagcg
     2941 atacatcgac gatcccgccg cggcggcttt gctggtggag aaggtggccc gcgcgatcca
     3001 tcacgcgcat cagcgcggca tcctgcaccg cgatttaaag ccggggaaca ttctgctgga
     3061 cagcgcgggt gagccgcacg tgagcgattt cggcttggcg aaatcgagcg agatggattc
     3121 ggggctgacg cagagcgaag cgtcgctggg cacaccgggg ttcatggcgc ccgagcaaat
     3181 ccgcggggcc aagagcatca cgacggcggc ggacatctac agcctggggg cgatcctcta
     3241 ccagttgctg accggcgtgc cgccgttcag cggggcttca ccgatggaag tgctgcgccg
     3301 cgtgacggat gaacagccgc cgccgccgcg gtcgcttcgc ccgcgcgtgc cgcgcgatct
     3361 ggaaacgatc tgcttgaaat gcctgcggaa agaaacgcac ctgcgatacg ccagtgccga
     3421 ggcgttggcg gatgaccttc ggcgatatcg cctgggcgag ccgatcgagg cgcgcacggt
     3481 cggcaagatc gaacggctgt ggagctggtg ccgccgcaag cccgcgttgg cggcggcga
//
LOCUS       DATIMH010000572         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_137751, whole genome shotgun sequence.
ACCESSION   DATIMH010000572 DATIMH010000000
VERSION     DATIMH010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137751"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..643)
                     /locus_tag="VLI90_09930"
     CDS             complement(2..643)
                     /locus_tag="VLI90_09930"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HSV14569.1"
                     /translation="MNIGVLGSGDVGTALAVGFLKYGHEVMVGTRDPAKLAAWRREHP
                     AAQVGSFSDTAKFGEVIVLAVKGTAASDALRAAGGAASLSGKTVIDAVNPIADEPPVD
                     GVLKFFTGPNESLMERLQKEFPTARFVKAFNSVGSALMVNPKFAEGRPTMFICGNDDA
                     AKRTVATILDQFGWETADMGKAEAARAIEPLCILWCIPGFLHNQWTHAFKLLK"
     gene            988..1881
                     /locus_tag="VLI90_09935"
     CDS             988..1881
                     /locus_tag="VLI90_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV14570.1"
                     /translation="MIQRKVLLAAIPALAVAALSPGAMANTHTTNGLFDPNEWTVGPT
                     DPTPVNPNSIPRQGFGFDATTHRGGAVLYVEQSTNGQGAVQKFGTTLDLMYDYIGSPS
                     ALGAGSNAGNSSVDIFFEVPNDADYAVHITNSGFTAFEKPNGTPSPENADGTLHFMDP
                     SSNPISPWTSLTTADLALANFHTALGFGASPNSAASHLIAEFDLTINSTGSPAGGGNP
                     GVYDPAPAFWSASGKSGTTDPPISSEIFNLQPDGTILASSVVNGAGDPVAQPSDVPEP
                     ATAGLFAIGASVLTMRRRRRD"
     gene            complement(2271..3164)
                     /locus_tag="VLI90_09940"
     CDS             complement(2271..3164)
                     /locus_tag="VLI90_09940"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HSV14571.1"
                     /translation="MSETILIFANPIAGRGRGRVVARRLERELTAAGYSAITILDRPA
                     DVKLSAEHRAATAAISIGGDGTLRGVVELLMAADGNAPPLLPVPMGTANLMGRHLGMK
                     WSAWNLGTSVLATLRRREIIQIDAARANGKLFLLMAGVGIDAQVVHLLDQMRRGPIDF
                     TSYVLPAAMTFANYDFPAITVNVDGQTVARDTPAIAVIGNVKEYGTGFPILTEARPDD
                     GLLDICVMPCRDRRALAEILMLVATGEHPLREDVVYLKGKSVRVESTTPVAVQIDGDS
                     AGYTPLQIDLLAQRVPFLVPA"
BASE COUNT          626 a         1074 c         1077 g          630 t
ORIGIN      
        1 ctcacttcag cagcttaaag gcgtgagtcc actgattgtg caggaatccg ggaatgcacc
       61 agagtatgca aaggggttcg atggcacgag cggcctcggc cttgcccatg tcggcggtct
      121 cccagccgaa ttgatcgagg atcgtcgcga ccgtgcgctt ggccgcgtcg tcgttgccgc
      181 agatgaacat cgtcggccgg ccttctgcaa acttcgggtt gaccatcaat gcgctgccaa
      241 cggaattgaa cgccttgacg aagcgggcgg tggggaactc cttctgaagg cgctccatca
      301 acgattcgtt cggccccgtg aagaacttga gcacaccatc gaccggcggt tcgtcggcaa
      361 tcgggttcac cgcatcaatg acggtcttgc cggacaagct tgccgcgcca ccggcggcgc
      421 gcagcgcatc cgatgccgcg gtgcctttga cggcgagcac gatgacttca ccaaacttcg
      481 ccgtgtcgct gaagctgccg acttgcgccg ccggatgctc acggcgccac gcggccagct
      541 ttgccgggtc gcgtgtgccc accatcacct catgaccgta tttcaaaaac ccgactgcca
      601 gcgcggttcc gacatcaccc gaaccaagga cgccgatgtt catgttgtct cctttttcaa
      661 ttccttagcg tgcacgcccg cgagcgtagg cgctattccg cccgacggca aattggatag
      721 ctacgagatt atgtagctat aatttgggaa aataacattg acttgatacg atttgtccaa
      781 taacatcaca ttggttaggg cagagggacg gttctcgcag ggtctgaccc gaccggggag
      841 gcggacaccg cgacacatca atcgccccgg gcattggttc ggctttcctg ccacttcgtt
      901 cgcgcggcaa cggtttcatt tttttcaccg aacgtgcttc cggcacgccc gcacgcggtc
      961 tgcgcacgtt cagaaggaga ggcagcgatg attcagcgga aggttcttct ggcggcgatt
     1021 ccggcgctgg cggtggcggc actgtcgccg ggcgccatgg ccaacacgca cacgaccaac
     1081 ggcctgttcg atcccaacga atggacggtc ggcccgaccg atcccacgcc cgttaatccg
     1141 aattcaatcc cgcgacaggg gttcggattc gacgcgacga cccatcgcgg cggcgcggtc
     1201 ttgtacgtcg aacagtccac caatggccag ggcgctgttc agaagtttgg aacaacgctc
     1261 gacctgatgt acgactacat cggcagcccg tcggctctgg gcgccggctc caatgccggc
     1321 aactcgtcgg tcgatatttt cttcgaagtg ccgaacgacg ccgactacgc cgtacacatc
     1381 accaactccg gcttcaccgc gttcgaaaaa ccgaatggca caccgtcgcc ggagaacgcc
     1441 gacggcacgt tgcacttcat ggaccccagt tccaatccga tcagcccctg gacgtcgctg
     1501 accaccgcgg acctggcgct ggcgaacttc cacaccgcgc tgggcttcgg cgccagcccc
     1561 aactcggcgg catcgcactt gatcgcggag ttcgacctga cgatcaacag caccggcagt
     1621 cccgccggtg gtggcaaccc cggcgtctac gaccccgccc ccgcgttctg gtcggcctcc
     1681 ggaaaatccg gcaccaccga tcccccgatc agcagcgaga tcttcaacct gcaaccggac
     1741 ggcaccatct tggcgagttc cgtcgtcaac ggcgcgggcg atcccgtggc ccaacccagc
     1801 gacgtgccgg aaccggcgac tgcgggatta ttcgcgatcg gcgccagcgt gctgacgatg
     1861 cggcggcgcc ggcgagattg attgttcgct ggcacaatag aaaagcccac ggctgtgccg
     1921 tgggaccggg tttcgaatcc ggtcccgcgg catggccgcg ggctttgtgc gtttgtcaga
     1981 tgtacttttt tggagggggg agaagcctgc tgtcatatct tgggtgcttg ctcgtcctga
     2041 ccgcagcacg gattcgtgaa ccattcgaag gacgttgcgg ctaagtaagg gaaggttcgc
     2101 ttgcgcaatg gccgccgctt gcggcttagc gtctttcgat gttccgaaac agatgctaag
     2161 ccgcaagcgg ctgtgacgcg ctctttgccg atggctcgtt caccaacgcc tcggcaaaat
     2221 gcgtcgactc cggcgactac gcccatgcgc agtatgacaa cgcgctcgct ctacgcgggc
     2281 accaggaacg gcacgcgctg cgccagcagg tcgatctgca gcggcgtgta accggcggag
     2341 tcgccgtcga tttggaccgc gacgggcgtg gtggattcga ctcggactga cttgcctttc
     2401 aggtacacca cgtcttcgcg caggggatgt tcgccggtgg cgacgagcat gaggatctcg
     2461 gccagcgccc ggcggtcgcg gcagggcatg acgcagatgt cgagtagccc gtcgtcgggg
     2521 cgggcttcgg tcaggatcgg gaagccggtg ccgtactcct tcacgttgcc gatgaccgcg
     2581 atggcgggcg tgtcgcgcgc gacggtttgg ccatccacgt tcaccgtgat ggcggggaaa
     2641 tcgtagttgg cgaacgtcat cgcggccggc aggacgtaac tggtgaagtc gatcggcccg
     2701 cggcgcatct ggtcgagcag gtgcacgacc tgcgcatcga tgccgacgcc ggccatcagc
     2761 agaaacaatt ttccattcgc gcgggcggcg tcaatctgga tgatctcgcg ccggcgcagc
     2821 gttgccagga ccgaagtgcc caggttccac gcggaccatt tcatgccgag atgccggccc
     2881 atgaggttgg cggtgcccat cggcaccggc agcaacggcg gggcgttgcc gtcggcggcc
     2941 atgagcagct cgaccacgcc gcggagcgtg ccgtcgccac cgatggagat ggcggcggtg
     3001 gcggcgcgat gttcggcgct gagcttcaca tcagcggggc gatcgaggat ggtgatcgcg
     3061 ctatagccgg cggcggtgag ttcgcgttcg agccggcgcg cgacgacgcg gccgcgcccg
     3121 cggccggcga tggggttggc gaagatgagg atcgtttcgg acatttggta atcgctggct
     3181 agaaaaagcg cgcggctgct aagtttctgt ccggtcccct ctcccacgta cgcgtgggag
     3241 agggttaggg tgagggtgat ttcaagaacc agtagctatc caaagactaa atcaccctca
     3301 ccccagccct ctcccacgac tacgtgggag agggggaaag aggcaagcgg acgcgcctta
     3361 acaagcgcgc gctcagtagc cttcgcgccg tgccgtccca cggcaga
//
LOCUS       DATIMH010000573         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_718036, whole genome shotgun sequence.
ACCESSION   DATIMH010000573 DATIMH010000000
VERSION     DATIMH010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718036"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..602)
                     /locus_tag="VLI90_09945"
     CDS             complement(219..602)
                     /locus_tag="VLI90_09945"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HSV14572.1"
                     /translation="MPYQVSKSEFGELVERALAELPEEFAEFLEEVPIEIRDHPTRQQ
                     MRSVNVPSDTGLLLGLYVGRPRTQRSVEHSGSMPDIIYIFQRPIETVSENEDQLIRQV
                     RTTVLHEIGHHFGMTEEDLRRLGYG"
     gene            687..>1558
                     /gene="sppA"
                     /locus_tag="VLI90_09950"
     CDS             687..>1558
                     /gene="sppA"
                     /locus_tag="VLI90_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSV14573.1"
                     /translation="MTIKTAVATILLFLTGCGTPSFLVTPVSNNSTLEETQVAGGKGW
                     FSGKIAIVEVEGMLANARTGGFLQPQENVLSLFTQELNRAASDDEVKAVVLRVNSPGG
                     TVTASDAMYQILQRFKARTHKPVVASAQELAASGAYYVSCGADRIVAQPTSIVGSIGV
                     IFETYNLSGTMNKLGIRPGAFKSAEHKDIGSPFRDPTPEEEQIMQGMVDEYYARFKSI
                     VRQNRQIPDDAAFKMMTDGRVFSGERAVALGLVDRTGLLEDAVQVARDLAHAPDASVI
                     AYKRPYGYGGSIYAL"
BASE COUNT          315 a          503 c          456 g          284 t
ORIGIN      
        1 ggcaggcgaa aagccacatg cacacgggtt taacgggtgg agaagcctct atacccagcg
       61 gccaaagcgt cggtcaacgc gacggggccg cacaaaaaac attaactatt tgccgccggt
      121 atagatcggt ccgcttgcgg aacaatcttg acgatctaac cgccgttacg cgaaaagctt
      181 gtagctcttg cgcgacgcgc tcggcggtga caactcccct acccataccc caaccggcgc
      241 aggtcctctt cagtcatgcc gaagtggtgg ccaatctcgt gtagtacggt cgttcgcacc
      301 tggcggatca gttgatcttc attttccgag acagtttcga tcggccgctg aaaaatgtag
      361 atgatgtccg gcatcgagcc cgagtgctcg acgctgcgct gcgtgcgcgg ccgaccgacg
      421 tacaggccca gcagaaggcc cgtatcgctc ggcacgttca ccgagcgcat ctgctggcgc
      481 gtcgggtgat cgcggatctc gatcggcacc tcttcgagga actccgcgaa ctcctccggc
      541 agctcggcca gcgcccgctc gaccagttcg ccgaactcac ttttgctgac ttgataaggc
      601 atcgctagaa tccgccgact cgccggttgg cacatccatg gtactccatc gatctcgcca
      661 gcgcgcagca ttcgggccta ttacctatga ccatcaaaac cgccgtcgcg acgatcctgc
      721 ttttcctcac cggctgcggc acgcccagct ttctggtcac gccggtttcc aacaacagca
      781 cgctggaaga gacccaagtc gccggcggca aaggttggtt cagcggaaag atcgccatcg
      841 tcgaagttga aggcatgctc gccaacgcgc gcaccggcgg gtttcttcag ccgcaggaaa
      901 acgtgctgag cctcttcacg caggagctga atcgcgccgc gagtgacgac gaagtgaaag
      961 ccgtcgtctt gcgcgtgaat tctcccggtg ggaccgtgac agcttcggat gcgatgtacc
     1021 agatcctgca gcgattcaag gcccgcacac acaagccggt cgtcgcctcc gcgcaggaac
     1081 tcgccgcgag cggtgcttac tatgtcagct gcggcgccga tcgcatcgtc gcgcagccca
     1141 ccagcatcgt cggcagcatc ggcgtcatct tcgagaccta taacttgtcc ggcaccatga
     1201 acaagctggg catccgcccc ggcgcgttca aaagcgccga gcacaaggac atcggctcgc
     1261 ccttccgcga tcccacgccc gaggaagagc agatcatgca ggggatggtg gatgaatatt
     1321 acgcgcggtt caaatcgatc gtccgccaga accgccagat cccggatgac gcggccttca
     1381 agatgatgac cgacggccgc gtgttcagcg gcgagcgcgc cgtcgcgctg ggcttggtcg
     1441 atcggaccgg cctgctcgaa gacgcggtgc aggtggcgcg agatcttgct cacgcgccgg
     1501 atgccagcgt catcgcgtac aaacggccgt atggatacgg cggatcgatc tacgcgct
//
LOCUS       DATIMH010000574         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3889209, whole genome shotgun sequence.
ACCESSION   DATIMH010000574 DATIMH010000000
VERSION     DATIMH010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3889209"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..462)
                     /locus_tag="VLI90_09955"
     CDS             complement(274..462)
                     /locus_tag="VLI90_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14574.1"
                     /translation="MIDMKVKVNAINTTGGDAAATNANGYAHHPAGALIHPRASHAAT
                     TSNHRRHHRPSRREIRFG"
     gene            472..1422
                     /locus_tag="VLI90_09960"
     CDS             472..1422
                     /locus_tag="VLI90_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSV14575.1"
                     /translation="MTRPVDWKCPSCRESNPANFDLCWNCGSDRAGVADPAFRPAIAF
                     HPVCETCGYLLVGLSENRCPECGHPFDPAKKDTPRALPAGVREAADAAAVMSTRDGYD
                     RWARVYDDDGNPLIALEEPLVDRLLGEVRGLGIADIGCGTGRHAIRLAQAGASVRGID
                     FSSAMLERARQKAGALPIQFTQHDLALPLPFAERRFDRVVCGLVVDHIADLTGLFGEM
                     RRICRADGFVIISVMHPAMMLRGVQARFRDPETGQEVRPASCEHQISDYVTAVTAAGL
                     EIVQLSEHAVDEALANRLERARRYLGWPMLFLMQLKPASS"
     gene            complement(1539..2051)
                     /locus_tag="VLI90_09965"
     CDS             complement(1539..2051)
                     /locus_tag="VLI90_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV14576.1"
                     /translation="MESGPVLIYRRIDPIADADVVYGNYRDACAASFGDAARCSTRAE
                     YLDWLTRRVEEFPDGHVLATLANRGGAIVGQLELQVPYGLRVGYVNLFHVTRDWRRLG
                     FGRQLHEEYADRYFRAWEADTIELHVSPTNHAAVQFYRSLGYKLAAAETRGDRMWRME
                     RRVERATAAS"
     gene            2230..>3260
                     /locus_tag="VLI90_09970"
     CDS             2230..>3260
                     /locus_tag="VLI90_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV14577.1"
                     /translation="MVVRKLAVSNLSVHRMRVALTVAAIALSVSLVVSVTSGYSSLAG
                     AAEKFMARFMGSADAMVTHQGDPHANIPESVVWDMRKDPDVKRVTGRLELESGLVNAK
                     GEPIAARPAQIIGVRRPEDDRVENLNIVKGDWFETASGNVALIDQVAAEKLGVNIGGT
                     FDLPGPSGSLPLKVVGIVHKPQILAAHIQSIYLPLETLQKFTMPDRPPQVNRVMIDLQ
                     PNANVDAFVERWKSKLEAVDPALKLRLARDNRKELARNLRGIQALSYLGGTVSMLAAT
                     FIVFSALSMGVTERSRMLAMLRAIGAVRWQIGRLVIAEGLLLASIGVAIGVPLGWVWI
                     KLLSLRFEH"
BASE COUNT          529 a          982 c         1157 g          592 t
ORIGIN      
        1 cgcgacggcc gctgcaagga actgtgaatc cctcaacgcc gggttcggag gtactccgac
       61 gagccggatg tgttggcgcg aagaatctcg cttcgtcgga gcacctccga gcccggcgtg
      121 ggcggttccg acacggtttc caatcaaatt ggctcatcca gattccaccc atcggaaatg
      181 cacgcggtcg ggcactacat cctcgtcgct gcctgcgtgc tcgtcgcgat gctcgcgctt
      241 ttggtcgcgg tgttctttgt ggctttggcc gattcacccg aagcgaatct cgcggcgcga
      301 cgggcggtgg tgccgacgat gattggacgt cgtcgccgca tggctggcgc ggggatggat
      361 caacgctccg gcgggatggt gggcgtaccc gttggcgttc gtggccgcgg cgtcgccgcc
      421 ggtggtgttg atcgcgttca ccttcacctt catgtcgatc aagaggcggc tatgaccaga
      481 cccgtcgatt ggaaatgccc gtcgtgccgc gagtccaatc ccgcgaactt cgatctgtgc
      541 tggaactgcg gcagcgatcg cgccggcgtg gcggacccgg cgtttaggcc ggcgatcgcg
      601 tttcatccag tctgtgagac gtgcggctac ctgctcgtcg ggctgagcga gaaccggtgt
      661 ccggagtgcg ggcatccgtt cgatccggcg aagaaggaca cgccacgggc gttgccggcg
      721 ggcgtgcgag aggcggccga cgcggcagcg gtcatgagca cgcgcgacgg ctacgaccgc
      781 tgggcgcgcg tatatgacga tgacggcaat ccgctgatcg cgctggagga gccgctggtc
      841 gatcggctgc tcggcgaggt gcgcgggttg ggcatcgccg acatcggctg cggcaccggg
      901 cggcacgcga tccggctcgc ccaggccggg gcgagcgtgc gcgggatcga cttctcttcg
      961 gcaatgctcg aacgcgcgcg acagaaggcg ggcgcgctgc cgattcagtt cacccagcat
     1021 gatctggcgc tgccgttgcc ctttgccgag aggcgattcg atcgcgtcgt atgcgggctc
     1081 gttgtcgatc acatcgccga cttgaccgga ctgttcggcg agatgcggcg gatctgccgg
     1141 gcggacgggt tcgtcatcat ctcggtcatg caccccgcga tgatgctgcg cggcgtgcag
     1201 gcgcgatttc gcgaccccga gaccggccag gaagtgcggc cggcgagttg cgagcaccag
     1261 atcagcgatt atgtgacggc ggtgactgcg gcggggcttg agatcgtgca gttgagcgag
     1321 catgcggtgg atgaggcgct ggcgaatcgg ctggagcgcg cgcgacgata tctggggtgg
     1381 ccgatgctgt tcttgatgca gttgaagccg gcgtcttcct gaccacgccg ggttcggagg
     1441 tactccgacg agccggattc tcctgagagt aaaaagcatc cgggtcgtcg cgagtacgct
     1501 ccgaacccgg cgtgggggtc gacggggaaa ctcttaaatc aactcgccgc ggtggcgcgt
     1561 tcgacgcggc gttccatgcg ccacatgcgg tcgccgcggg tttcagcggc ggcgagcttg
     1621 tagcccagcg agcgataaaa ctgcaccgcc gcgtggttgg tcggcgagac gtgcagctcg
     1681 atcgtgtcgg cttcccaggc gcggaagtag cggtcggcgt attcctcgtg cagctgccgg
     1741 ccgaacccga ggcgacgcca gtcgcgcgtg acgtggaaca ggttcacgta accgacgcgc
     1801 aggccgtagg gcacctgcag ttcgagctgg ccgacgatcg ccccgccccg gttggcgagc
     1861 gtggccagca cgtgaccgtc ggggaactct tcgactcggc gggtgagcca gtcgaggtat
     1921 tcggcccgcg tgctgcagcg cgcggcgtca ccaaagctgg cggcgcaggc gtcgcgatag
     1981 ttgccgtaga cgacgtcggc gtcggcgatc ggatcgatcc ggcggtagat caggacaggc
     2041 ccgctttcca cgtcataaca atagccgcgc tgaggacggt gaatccatca agcttttgct
     2101 gcgttgcgca gcggtagccc cgcaagggca cgtgtttctt cttgaacgca gaaacctgtg
     2161 cccctgcggg ctaccgccac gcagcacttt gatgcgctcg cgcgccgcgt taccgctaca
     2221 atccccgcca tggttgttcg caagctcgcg gtttccaatc tgtccgtcca ccgcatgcgg
     2281 gtcgcgctga ccgtcgcggc gattgcgctg tcggtcagcc tcgtcgtgtc ggtgaccagc
     2341 ggatactcat cgctggccgg ggcggcggaa aaattcatgg cccgcttcat gggcagcgct
     2401 gatgcgatgg taacgcacca aggggacccg cacgcgaaca tcccggaatc ggtcgtctgg
     2461 gacatgcgca aagatccgga cgtcaagcgc gtcaccgggc ggttggaact ggagagcggc
     2521 ctggtcaacg ccaagggcga accgatcgcc gctcggccgg cgcagatcat cggcgtgcgg
     2581 cggccggagg atgaccgcgt tgagaatttg aacatcgtca aaggcgattg gtttgagacg
     2641 gccagcggga acgtcgcgct gatcgaccag gtggcagcgg agaagctggg ggtgaacatc
     2701 ggcggcacgt tcgatctgcc ggggccgagc gggtcgttgc cgctgaaggt ggtggggatc
     2761 gttcacaagc cgcagattct ggccgcgcac atccagtcga tttatttgcc gctggagacg
     2821 ctgcagaagt tcacgatgcc cgaccgtccg ccacaggtaa accgggtgat gatcgacctg
     2881 cagccgaacg cgaacgtcga tgcgttcgtc gagcgatgga aatcgaaact ggaggcggtc
     2941 gatcccgcgc tgaagctgcg gctggcccgc gacaaccgca aggaattggc ccggaatttg
     3001 cgcggcattc aagcgctcag ctacctcggc gggaccgtct cgatgctcgc cgcgacgttc
     3061 atcgtcttca gcgcgttaag catgggcgtc accgaacgct cgcgcatgct ggcgatgctg
     3121 cgggcgatcg gcgcggtgcg ctggcagatc ggccggctgg tcatcgccga gggcttgctg
     3181 ctggcgagca tcggggtcgc gatcggtgtg ccgctgggtt gggtttggat caagctgctg
     3241 tcgctgaggt tcgagcacat
//
LOCUS       DATIMH010000575         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1873717, whole genome shotgun sequence.
ACCESSION   DATIMH010000575 DATIMH010000000
VERSION     DATIMH010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873717"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VLI90_09975"
     CDS             <1..608
                     /locus_tag="VLI90_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14578.1"
                     /translation="LMPFFAGGLYRSFFTLPRPTSWRALLRPGMIVPLKSRAGVARAC
                     LLITAIALFVGGSTIMTVGMTRVFVPQDLEFMGLTADQIRAINPRLVPLIAHDRAGFG
                     GGLCATGVTVFFCVWCAAPSRSLWQVLCVAGIAGFGTAIFIHPIVGYLSVSHLLPAVC
                     VAVMLVVGLCLSFGPMMRAQRLHSFPPSGESLSGRKELEHA"
     gene            601..729
                     /locus_tag="VLI90_09980"
     CDS             601..729
                     /locus_tag="VLI90_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14579.1"
                     /translation="MLETATMLIPVAAAGWAFLYWIFGGGFFGAIILFVVLKMFGK"
     gene            919..1452
                     /locus_tag="VLI90_09985"
     CDS             919..1452
                     /locus_tag="VLI90_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14580.1"
                     /translation="MFGFGKQQQEQPPGPRVNARLCTDKFSGKYPHVGLFDCRARKVW
                     ISRPLNGQAIRTSHARLITGAENAVSTVWKDRFLCFWFYTPRTGEGYIHGYPIEWDEA
                     HLLVRIDPHWDYDRQRMVPPELADHVDDNLERQFRHGERILEFYRTAKLKYPFSLHYI
                     GQQATDSQFYVRRVEAP"
     gene            1648..1983
                     /locus_tag="VLI90_09990"
     CDS             1648..1983
                     /locus_tag="VLI90_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HSV14581.1"
                     /translation="MPVLRSTPTSTPASVTEVLTPQGELKRILRLQVPVIVKLAERKL
                     LLSEVMRLGVGAIIEFIKSSEEPLELLINNKPIGVGEAVKVGENFGLRIKQIGDVKQV
                     IASLGSDHR"
     gene            complement(2007..>2822)
                     /locus_tag="VLI90_09995"
     CDS             complement(2007..>2822)
                     /locus_tag="VLI90_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14582.1"
                     /translation="RAVRFASRFDFEIEPVTAAAVRAHAHQLPRISPERIADELRRML
                     TAPSHNVAWRFLWDLTLVRQLFRTMVISGDTAFDPARSIFLALPYSGSSISFGLALAA
                     ATLDYRWQLSERSADVRAWLTKTEIGKTVRAMRQALKISNEESDELEGTLSGAAAMLA
                     EPAAGVAGMKRFLATPAAGLARQLMAALAGVGLFCDRIDQVERALAALERTEFAPAPL
                     ITGDDLTAVGATPGPAFKRALDAVYDAQLEGNISSRAEALEMGLLILSRLGGS"
BASE COUNT          514 a          977 c          822 g          509 t
ORIGIN      
        1 tgctgatgcc gttcttcgcg ggcgggttgt accgctcgtt cttcacgctc ccgcggccga
       61 ccagctggcg cgcgctgctg cgcccgggca tgatcgtccc tttgaaatcg cgcgccggcg
      121 tcgcgcgcgc ctgcctatta atcaccgcca tcgcgctctt tgtcggcgga tcgaccatca
      181 tgaccgtcgg catgacgcgc gtgttcgtcc cgcaagacct ggagttcatg ggcctgaccg
      241 ccgaccagat ccgcgcgatc aacccgcggc tggtgccgct gatcgcccac gaccgggccg
      301 gtttcggcgg cggcctctgc gccaccggcg tcaccgtctt cttctgcgtc tggtgcgcgg
      361 cgccgtcgcg cagcctctgg caggtgctgt gcgtcgccgg catcgccggg ttcggcacgg
      421 cgatcttcat ccatccgatc gtcggttacc tcagcgtctc gcacctgctg cccgcggtgt
      481 gcgtggcggt gatgctggtg gttggtttat gcctgagctt cggccccatg atgcgggcac
      541 agcgtttgca ctcgttccct ccgtcgggcg aatccctgtc cggcagaaag gaacttgaac
      601 atgcttgaaa ccgcaaccat gctgattccc gtcgccgcag cggggtgggc atttttatat
      661 tggatcttcg gcggtggctt cttcggcgca atcatcctgt tcgtcgtgct gaagatgttc
      721 ggtaaatgac cccgtgtccg ccgatttaga gatgcagaga accgccggtg gtgctccacc
      781 ggcggttctc gcgcgccggg tgcggacgtc tggccaatct gtcgccggtg cgccgattga
      841 acggcacagt tcgccgtttg cgttttgtgc cgctgcttct attcttacgc ccaaccgcgc
      901 agccaaaggg caccacgcat gtttgggttc ggaaagcagc agcaggagca gccgcccggc
      961 ccgcgcgtca acgcccgcct ctgcaccgat aaattctccg gcaaatatcc ccacgtcggc
     1021 ttgttcgatt gccgcgccag aaaagtctgg atctcccggc cgttgaatgg ccaggccatt
     1081 cgcacatcgc acgcgcgcct gatcaccggc gcggagaacg ccgtcagcac ggtgtggaag
     1141 gatcggtttc tatgtttctg gttctacacg ccccggaccg gcgaggggta catccacggc
     1201 tatccgatcg aatgggacga ggcgcacctg ctggtgcgga tcgacccgca ctgggactac
     1261 gaccgccagc gcatggtgcc gccggagttg gccgatcacg tggacgacaa cctcgagcgc
     1321 cagttccgcc acggcgagcg gatcctggag ttctatcgca ccgctaagct gaagtacccc
     1381 ttcagcctgc attacatcgg acagcaggcg acggactcgc agttttatgt acgccgcgtg
     1441 gaagcgccgt gaaccgcgcg cagaatcgat catttgagta acgcccgacg ccggtgtctg
     1501 agggtactcc cgaagacccg ggtccgtgcg ccgaagatac ccgttcttcg ggagtaccct
     1561 cagacaccgg cgtcacgggc atagcgcgag cttcggaacg cacgctgttg agaaccaccg
     1621 cgttgtgccg ataatgttca aaccaacgtg cctgtcctgc gctccacgcc aacctcaacg
     1681 cccgcttcgg tgaccgaagt cctgacgccg cagggcgaac tcaaacgcat cctgcgcctg
     1741 caggtgccgg tgatcgtcaa gctggctgag cgaaagctgc tgctgtcgga agtgatgcgc
     1801 ctgggcgtcg gcgcgatcat cgaattcatc aaaagcagcg aagaaccgct ggagctgttg
     1861 atcaacaaca aacccatcgg cgtaggcgaa gcggtaaaag tcggcgaaaa cttcggcctg
     1921 cgcatcaaac aaatcggcga cgtgaagcaa gtcatcgcgt ccctgggaag cgatcaccgc
     1981 taaccacggg ctaaccgctt cagcggttag cttcccccca agcgcgaaag aatcaaaagc
     2041 cccatctcca gcgcttccgc ccgagaagaa atgttcccct ccaactgcgc atcataaacc
     2101 gcatccagcg cccgcttaaa cgcaggcccc ggcgtcgccc ccaccgcggt cagatcatcg
     2161 ccagtaatca acggcgccgg cgcaaactcc gtccgctcca acgccgcaag cgcccgctca
     2221 acttgatcga tccgatcgca aaacaatccc acgcccgcca gcgccgccat cagttgccgc
     2281 gccaaccccg ccgccggcgt cgccagaaac cgcttcatcc ccgccacgcc cgccgccggt
     2341 tccgcgagca tcgcggccgc gccgctgagc gttccctcca gttcatcgct ctcttcattg
     2401 ctgatcttca acgcctgccg catcgcgcgg accgtcttgc cgatctccgt cttggtcaac
     2461 cacgcccgca cgtcagccga tcgctccgac aactgccagc gataatccaa ggtcgccgcg
     2521 gcgagcgcga ggccgaatga gatggatgat cccgaatacg gcagggcgag gaagatcgat
     2581 cgcgcgggat cgaacgcggt atcgcccgag atgaccatcg tccgaaacag ctgccgcacc
     2641 agcgtcagat cccacaagaa ccgccacgcg acgttgtgcg acggcgccgt caacatccgc
     2701 cgcagctcat ccgcaattcg ttccgggctg atccgcggca gctgatgcgc gtgcgcccga
     2761 accgccgccg ccgtcaccgg ctcgatctca aaatcgaacc gcgacgcgaa gcgaacggcg
     2821 cg
//
LOCUS       DATIMH010000576         1786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4762568, whole genome shotgun sequence.
ACCESSION   DATIMH010000576 DATIMH010000000
VERSION     DATIMH010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4762568"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..>1786)
                     /locus_tag="VLI90_10000"
     CDS             complement(265..>1786)
                     /locus_tag="VLI90_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSV14583.1"
                     /translation="KDTIIVGSSFREGATVSTHNNTKGLVRAFDARTGKLLWTFNTIP
                     RPGEFGNDTWENDSWAVNGNTGVWTQITADEDLGLVYLPVETPTSDFYGGHRPGNNLF
                     AESLVAVDLRTGQRKWHYQVVHHPLWNYDMSSAAMMADITVNGRAIKAVAVPSKQGFL
                     WVLDRATGQPVWPIEERPVPQSDVPGEKTSPTQPFPTRPPAYTRTTLKVPDDLVDFTP
                     AMREQAVKQIGRYKVGPWMYNPAVLGDVNGLLGAINMGNAVGGTNWPGGGYDPDTHTA
                     FTPGNNVGITAATLTPPPAGFSDIRYVSGVAGRPFQEVLGPGDCCAADSPRASTTETR
                     DPLTGSPRPQPAPAPAPAPAAAANPGGLAIDGISIIKPPYGLISAINLDRGEIVWQAV
                     HGDTPDNIRNHPALRGMNIPKTGQAGTSGIGLLVTKTLVIMGDPALTTTPDHPRGAMF
                     RAYDKADGKEVGAVFMVAPQSGSPMTYMADGKQYILNAISGGSYSGEYIAFSLPAK"
BASE COUNT          282 a          539 c          611 g          354 t
ORIGIN      
        1 atctctaaat ggcgttatgc acagcgcttc ccggcaaagg tagcgctggc gatgcgtttg
       61 tttttgaggc gggagagaga cctgaactcg tgcggagtga gggccgatct ttgttgtttg
      121 tttacgaaat gccgaactga acggaccgac aaatcgtcgt gcgcgtctca gtggctgacc
      181 tggaggaagt cccgcgccac tgttcgtctc ttctgaacaa atggcgcggg cctgaagagc
      241 aggtcgcggc ggagaacgga agcgctattt tgcggggaga gaaaacgcga tgtactcgcc
      301 ggaatagctg ccgccgctga tggcgttgag gatgtactgc ttgccgtcgg ccatgtaggt
      361 catcggcgaa ccgctctgcg gtgccaccat gaacacggcg ccgacctctt tgccgtccgc
      421 tttgtcatag gcccggaaca tcgcaccccg tgggtgatcg ggggtggtgg tcagcgccgg
      481 gtcgcccatg atgaccagcg tcttcgtcac gagaaggccg atgccggacg tgcccgcctg
      541 cccggtcttc ggaatgttca ttccgcgcag tgccgggtgg ttgcggatgt tgtccggcgt
      601 gtcgccatgc acggcctgcc agacgatctc gccgcgatcg aggttgatcg ccgagatcag
      661 gccgtagggc ggcttgatga tcgagatccc gtcgatcgcg agtcctcccg ggtttgctgc
      721 agcggcggga gccggtgcgg gtgccggagc gggctgaggc ctcggcgatc cggtgagcgg
      781 atcgcgcgtt tccgtcgttg acgcacgcgg cgagtcggct gcgcagcagt cgcctggtcc
      841 gagcacttcc tggaacggcc ggccggcgac accggacacg tagcggatat ccgaaaagcc
      901 cgccggcgga ggcgtcagcg tcgccgcggt gatgccgacg ttgtttcccg gcgtgaacgc
      961 ggtatgcgtg tccgggtcgt agccgccacc gggccagttc gtgcctccga cggcattgcc
     1021 catgttgatg gcgccgagca ggccgttcac atcgccgagg accgccgggt tgtacatcca
     1081 gggcccgacc ttgtagcgcc cgatttgctt gacggcctgc tcccgcatcg ccggagtgaa
     1141 gtcgaccaga tcgtccggca ccttcagcgt cgtgcgcgtg taggcaggtg gcctggtcgg
     1201 gaacggctgc gtcgggctgg tcttctctcc tggtacatca gactggggaa ccgggcgttc
     1261 ctcgattggc cacacaggct gcccggtcgc gcgatcgagc acccagagga aaccctgttt
     1321 gctcggcacg gcgacagcct tgatcgcgcg gccgttcacc gtgatgtcgg ccatcatcgc
     1381 ggcggatgac atgtcgtagt tccacagcgg atggtgaacg acctggtagt gccacttgcg
     1441 ctggccagtc cgcagatcga cggcgaccag gctctcggcg aagaggttgt tgcccgggcg
     1501 gtgaccgccg tagaaatcgg acgtcggcgt ttcgacgggc aggtatacga ggcccaggtc
     1561 ctcgtcggcg gtgatctggg tccacacgcc ggtgttgccg ttcaccgccc acgaatcgtt
     1621 ttcccacgtg tcgtttccga attcacccgg ccgtggaatc gtgttgaagg tccagagcag
     1681 cttgcccgta cgcgcgtcga aggcacgcac caggcccttg gtgttgttat gcgtgctcac
     1741 ggtcgcccct tcgcggaacg acgagccgac gatgatcgtg tccttg
//
LOCUS       DATIMH010000577         1694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5626448, whole genome shotgun sequence.
ACCESSION   DATIMH010000577 DATIMH010000000
VERSION     DATIMH010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5626448"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..>1694
                     /locus_tag="VLI90_10005"
     CDS             31..>1694
                     /locus_tag="VLI90_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HSV14584.1"
                     /translation="MSLVELSKSMPELLAPAGDWDAMRAAVANGANAVYFGLENFNAR
                     HRATNFTLEELLRIMSFLHDHNVKGLLTFNTLIFSDEMPAAVEYIRAIAAAGVDAVIV
                     QDLGLARLIHAMAPTLHVHGSTQMTLTEPRGIEFVRRLGVQRVVLARELSVADIRKIR
                     GGTTMPLEVFVHGALCVAYSGQCLTSEALGGRSANRGQCAQACRLPYDLLVDGEVKDL
                     GEKAYLLSPQDLAAYDLIDELADVGVASFKIEGRLKSAHYVAATTQTYREAVDAAAGG
                     GQFRIGPQQRADLEQTFSRGFSHGFLSGVDHQALVVGRFPKSRGRRIGTVERASAKGI
                     SIKVEINEPSPLKLGDGVVFDEGHPEQDEQGGRVFAVRPAGRGRIEIELGRGDVNAAA
                     IANGSIVWKTDDPALRRRLEHSFSRDEVVHRAPLTAELHAHVGERLRLRLSDGQAEAS
                     VEWDKPLERAMKIPLTEAVAREQLARLGYTPYQLAELRLDAAGEPMVPKSVLNDLRRR
                     GVEQLLAERARRRPHAIADGNALSRLRSGAKSSPSPGIPGEGRGEGS"
BASE COUNT          284 a          498 c          620 g          292 t
ORIGIN      
        1 ttctgaattc gcacgatgga gcccgcacct gtgtcacttg tcgagttgtc gaagtccatg
       61 ccggaactgc tcgcacccgc gggggattgg gacgcgatgc gcgcggccgt ggctaatggc
      121 gcgaacgcag tttacttcgg cctggaaaac ttcaacgccc ggcatcgcgc gacgaacttc
      181 accctggaag agctgctgcg gatcatgtcg ttccttcacg atcacaacgt gaaggggctt
      241 ctcacgttca acacgctgat cttttccgac gaaatgccgg cggccgttga gtacatccgc
      301 gctattgccg cggcgggggt ggacgcggtt attgtgcagg acctgggact ggcccggctg
      361 attcacgcca tggcgccgac gctgcacgtg catggatcaa ctcagatgac gctgacggag
      421 ccgcgtggca tcgagttcgt ccggcggctg ggcgtgcagc gcgtcgtgct ggcgcgcgag
      481 ctgagcgttg ccgacattcg aaagattcgc gggggcacga cgatgccatt ggaagtgttc
      541 gtccatggcg cgctttgcgt cgcttactcg ggtcaatgcc tgacgagcga ggccctgggc
      601 ggccgcagcg ccaaccgcgg gcaatgcgcg caggcgtgcc gattgccgta cgacttgctg
      661 gtcgatggcg aagtgaagga tctgggcgag aaggcatacc tgctttcccc gcaggatctg
      721 gcggcgtacg acttaatcga cgagctagct gacgttggcg tggcgagctt caagatcgaa
      781 gggcggctca agagcgcgca ctacgtcgcg gcgacgacgc agacctatcg cgaggccgtc
      841 gatgccgccg cgggcggcgg gcagttccgc atcgggccgc aacagcgggc ggatctggaa
      901 cagaccttct cgcgaggctt cagccacggc tttctctccg gcgtcgatca ccaggcgctg
      961 gtcgtcgggc gattccccaa gtcgcgcggc cggcggattg ggaccgtcga acgcgcatcc
     1021 gccaaaggca tttcgatcaa ggtcgaaatc aacgaaccat caccgctgaa gctgggcgac
     1081 ggcgtcgtct tcgacgaagg ccacccggag caagacgagc agggtggccg cgtcttcgcg
     1141 gtgcgtccgg cgggacgcgg gcggatcgag atcgagctgg gccgcggtga cgtgaatgcg
     1201 gcggcgatcg ccaatggctc gatcgtgtgg aagacagacg acccggcgct gcggcggcgg
     1261 ttggagcatt cgttctcgcg cgatgaggtg gtccatcgcg ctccgctgac ggcggaactg
     1321 catgcgcatg tgggcgagcg tttgcgcctt cgcctgagcg atggacaggc ggaagcttcg
     1381 gtggagtggg acaaaccgct ggagcgcgcg atgaagatcc cgctgaccga ggcggtggcg
     1441 cgcgagcagt tggcgcggtt gggctacacg ccgtatcagt tggcggaact gcggctggac
     1501 gcggcgggcg agccgatggt gccgaagtcg gtgctgaacg acctgcggcg gcgcggcgtg
     1561 gagcagttgc tggcggaacg ggcgcggcgg cggccgcatg cgattgcgga tgggaacgcg
     1621 ctgtcgcgac ttcgcagcgg ggcaaagtct tccccctctc ccggtatccc gggagagggc
     1681 cggggtgagg gttc
//
LOCUS       DATIMH010000578         5492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1874833, whole genome shotgun sequence.
ACCESSION   DATIMH010000578 DATIMH010000000
VERSION     DATIMH010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874833"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1450)
                     /locus_tag="VLI90_10010"
     CDS             complement(92..1450)
                     /locus_tag="VLI90_10010"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HSV14585.1"
                     /translation="MSNVLRIANAHAFWGDDAGAAARLAAASPALDWITLDYLAEVSM
                     SILAKQRERDPSAGYARDFLDVVESLIPLWNSGHRVRIVTNAGGLNPAGCAAACAELL
                     KRAGCGGVKIGVVSGDDVLGQLRADAQAFRHLETGDAPDSVASRLVTANAYLGAAPIV
                     GAILAGAAIVITGRVADPSLTVAPCIAHFGWSNDDYASIAGATVAGHLIECGTQVTGG
                     ISTDWLDVPDAANIGFPIAEVAADGSCVIAKPPGTGGRVSEQTIKEQLLYEIGDPARY
                     LSPDATVSFLTLRVSDEGNDRVRVSGATGSAPPATYKVSATFRAGFRASGTLTVFGRD
                     AVAKAKRCGVIVLQRLKDRGCSPRQSWVECLGAGAVAPGVLPAHRAGATETVLRITVA
                     DDGRDVVERFTRELMPLISAGPQGITGYAEGRPVVREVFGYWPTLIARQRVQPRVEMI
                     DC"
     gene            complement(1515..3158)
                     /locus_tag="VLI90_10015"
     CDS             complement(1515..3158)
                     /locus_tag="VLI90_10015"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HSV14586.1"
                     /translation="MTKRSADPRASSLGNPRADLTLRSLTADIARQEAELRKGGGATG
                     QERQRKLNRLPVRERLAKLLDGDAALFLELGLWAAHEMYPDIGDVPAAGVVTGVGSIE
                     GRGCMIVANDATVKAGAFFPATVKKVLRAQRIAARCNLPLVYLVDSAGVYLPMQDEIF
                     PDEDDFGRIFRNNAVLSAMGVPQFAAIMGNCVAGGAYLPVLCDKVLMTDGSELDLAGP
                     ALVKAAIGQDVGREELGGATMHARVSGTIDFHEPNDEVCLERLRSLVALLPNDPPSAQ
                     HFAAPARDVDDIYTIVNGHGEFDVRDVLACIADADSIQEYKAEYGKTLVCAYARVGGH
                     KIGVVANQRTRAKSANGELQIGGVIYHDSADKAARFIMDCNQTGLPLVFLQDVTGFMV
                     GRDAEQHGIIRSGAKLVNAVSNSTVPKITVVIGGSFGAGNYALCGKAFDPAFLFAWPG
                     ARYAVMGAEQAAETILSIERRAADRAGRKLADDELKQLHDEIKRDYQRQTDIRYGAAR
                     GWVDAIIAPHATRDVLVHALRCATRPPPHGGFRAGVMQV"
     gene            complement(3367..4284)
                     /locus_tag="VLI90_10020"
     CDS             complement(3367..4284)
                     /locus_tag="VLI90_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSV14587.1"
                     /translation="MASEPRYKWQLLRAGPLRLDGGSMFGVVPRAVWSRVAPADEMGR
                     IECAHNCLLLEPATPDAREPKRILIEVGSGDKFDEKNRKIFGLGDYSIIDAVRDAGGS
                     CEAIDHVIVSHLHFDHAGGLTRAPDARSTFPNATIHVQRREWQDANANRSVMTRTYLR
                     ENFGSIREQLHLIDSLPPFPPGHLPQRDELPATPLSERETEILPGIRVFNIPGHTWGQ
                     HAIRFTDDRGRTVVFSADLLPTANHVGAAYNMAYDVEPFMSTVTRRWFLKEAAARDWL
                     LVLDHEPHKPLQRVRENAGGWYQLSEETS"
     gene            complement(4363..>5492)
                     /locus_tag="VLI90_10025"
     CDS             complement(4363..>5492)
                     /locus_tag="VLI90_10025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSV14588.1"
                     /translation="KMETAKALVDVPSPRGGIIAMLHGKPGQAIKVGAPLVTFKGDVS
                     SSGNGAPRNAPAVEERRDAGSVVGDLGDATAIEAEGKSLAAPAVRRLARDMGIELSHV
                     HGTGIGGRITAGDVQAAVAEQDQPAAHKRKPAAKQPANPAASLRTPFRGVRRTIAEHL
                     HHSITHAVHFTVMDEADVTALDALRRKLVAASNEKISLLPFVAVAICRVLSGRSGAKF
                     TRLNSTVDDEHQEIVQHRAVHLGIATDTESGLMVPVIRDAEHIGVLELSRMIAQLAQA
                     ARGRELSPDQLTGSTFTISNFGAMGGRFGTPIINYPEAAILAIGRAREGVVVRDGMLG
                     VGKLLPLSLSADHRVIDGGTAAAALGKIVSLLQTPDELLPT"
BASE COUNT          870 a         1844 c         1821 g          957 t
ORIGIN      
        1 cggctttgcc gtgggacgtc tttcaagcac cagtcccacg gcagagccag gggcttttgt
       61 tcagaatctg accgcccatc atcgttcatc attagcaatc gatcatctcg acccgtggtt
      121 gcacgcgctg gcgcgcaatt agcgtcggcc agtaaccgaa cacttctcgc acaactggcc
      181 ggccctcggc gtagccggtg attccctgcg ggccggcact gatcagcggc atcaactcgc
      241 gcgtgaatcg ctcgaccaca tcgcggccat catccgcgac ggtgattcta agcacggtct
      301 ccgtcgcgcc cgcgcgatgc gccggcaaca cgccgggcgc aaccgcgccg gcgccgaggc
      361 attccaccca cgattgccgc ggcgagcagc cgcggtcttt caggcgttgc aggacgatca
      421 cgccgcaccg cttggctttg gcgaccgcgt cgcggccgaa gaccgtcagc gtgccggagg
      481 cgcgaaagcc ggcgcgaaac gttgcgctga ccttgtacgt cgccggcggc gctgagccgg
      541 tcgcgccgct gacgcgaacg cgatcgttgc cttcgtcgct gacgcgcagc gtgaggaagg
      601 acacggtggc atcggggctg agatatcgcg ccgggtcgcc gatctcgtac agcagttgct
      661 ccttgatggt ctgctcgctc acgcgcccgc cggtgccggg cggtttggcg atgacgcacg
      721 aaccatcggc ggcaacttcg gcgatcggaa agccgatgtt cgcggcgtcc ggcacgtcga
      781 gccagtcggt gctgatcccg cccgtcacct gcgtcccgca ttcgatcaga tgcccggcga
      841 ccgttgcgcc ggcgatggaa gcataatcgt cgttcgacca gccgaagtgc gcgatgcatg
      901 gggcgacagt caggctggga tcggcgacgc ggccggtgat gacgatcgcg gcgccggcga
      961 ggatcgctcc gacgattggt gcagcgccga gatacgcgtt ggccgtcacc aggcggctgg
     1021 cgacgctgtc cggcgcatcg cccgtctcaa gatggcgaaa tgcttgggca tccgcgcgca
     1081 gttgcccgag cacatcatcg ccgctgacga cgccgatctt cacgccgccg cagccggctc
     1141 gcttcagcag ttccgcgcac gcggcggcgc atccggccgg attcaacccg cctgcgttgg
     1201 tgacaatgcg cacgcggtgg ccggaattcc acaatggtat gagcgactcg acgacatcga
     1261 ggaaatccct cgcgtaaccc gccgaaggat ctcgctcgcg ctgcttggca aggatcgaca
     1321 tcgatacttc cgccaggtaa tccagcgtga tccagtcaag cgccggcgac gccgccgcca
     1381 ggcgcgcggc cgcgccggcg tcatcgcccc agaaggcgtg tgcattggcg attcttaaga
     1441 cgttgctcat acgtagcacg ggcgtctcgc ccgtgtcgaa cggcgccagg cacgggcgag
     1501 acgcccgtgc tgcatcacac ctgcatcacg cctgccctaa atccaccatg cggcggcggg
     1561 cgtgttgcac atcgcagcgc atgcaccagc acgtcgcgcg tcgcgtgcgg ggcgatgatc
     1621 gcatccaccc acccgcgggc ggcgccgtag cgaatgtcgg tctgccgctg gtaatcgcgc
     1681 ttgatctcgt cgtgaagttg tttcaactcg tcatccgcca gcttccggcc ggcgcgatcc
     1741 gcggcgcgcc gctcaatcga caggatcgtc tcggccgcct gttcggcgcc catgacggcg
     1801 tagcgcgccc cgggccaggc gaatagaaaa gcgggatcga atgccttgcc gcacagcgcg
     1861 tagttaccgg cgccgaagct gccgccgatg acgaccgtga tcttcggcac ggtcgaattg
     1921 ctcaccgcgt tcacgagctt cgctccgctg cggatgatgc cgtgctgctc ggcgtcccgg
     1981 ccgaccatga agccggtcac gtcctgcagg aacaccagcg gcaggccggt ctggttgcaa
     2041 tccatgatga agcgggcggc cttgtcggcg ctgtcgtgat aaatcacgcc gccgatctgc
     2101 agttcgccgt tcgcgctttt ggcgcgcgtg cgctggtttg cgacaacgcc gatcttatgc
     2161 ccgccgacgc gcgcgtaggc gcagacgagc gtcttgccgt attcggcctt gtattcctga
     2221 atcgagtcgg cgtcggcgat gcacgcgagc acgtcgcgca cgtcgaactc gccgtggccg
     2281 ttcacgatgg tgtagatgtc gtcaacgtcg cgggccggcg ccgcaaaatg ctgcgcggac
     2341 ggcggatcgt tcggcagcag cgcgaccagc gaccgcaggc gctctaagca cacttcgtcg
     2401 ttcggctcgt gaaaatcgat cgtgccgctc acgcgggcgt gcatcgtcgc gccgcccagc
     2461 tcttcgcgac caacatcctg cccgatggcc gccttcacta gcgccggccc ggccagatcc
     2521 agctcgctgc cgtcggtcat cagcaccttg tcgcacagca ccggcaggta cgcgccaccg
     2581 gcgacgcagt tgcccatgat ggctgcgaac tgcggcacgc ccatcgcgct cagcaccgcg
     2641 ttgttgcgga aaatccggcc gaaatcgtct tcgtccggaa aaatctcgtc ctgcatcggc
     2701 aggtaaacgc cggccgaatc gaccaggtat accagcggca aattgcagcg agcggcgatg
     2761 cgctgcgccc gcagcacttt cttcacggtc gccgggaaaa acgcgccggc cttcaccgtc
     2821 gcatcgttgg cgacgatcat gcagcctcgc ccttcgatcg agccgacacc ggtgaccaca
     2881 cccgccgcgg ggacatcgcc gatatcgggg tacatctcgt gagcggccca gaggccgagc
     2941 tcgaggaaca gggcggcgtc gccatcgagc agcttagcca ggcgctcgcg caccggcagg
     3001 cggttgagct tccgctgcct ctcctgcccg gtcgcgccgc cgcctttgcg cagctcggcc
     3061 tcctggcgcg cgatgtcggc cgtgagggat cgcagcgtga ggtccgcgcg tggattgccg
     3121 agcgacgacg cgcgcgggtc tgccgatcgc ttcgtcacag ctcacctccg cgggcagagg
     3181 atcgatgcga gcggcgcgct tcatagccgc aggcgacagc cagcgggtgc gcgcccagtc
     3241 gaacccggtg gtgattccaa cgcccccgct ggctttcgcc tgcggctgtc aggggttccc
     3301 gtcctgcggc gcgcgcatca ctgcgaaggg aatgcgaaag caagagtggc aatggccgcc
     3361 gttgcctcac gacgtttcct ccgacaactg ataccatccg cctgcgttct cacgcacgcg
     3421 ctgcagcggt ttgtgcggct cgtggtccag caccagcagc caatcgcgcg cggcggcttc
     3481 tttcaagaac caccgtcgcg tcacggtgct catgaacggc tcaacgtcat acgccatgtt
     3541 gtacgcggcg ccgacgtggt tcgccgtcgg cagcagatcg gcgctgaaga cgaccgtgcg
     3601 gccgcgatca tcggtgaagc ggatcgcgtg ctggccccag gtgtgaccgg ggatgttgaa
     3661 gacgcggatg ccggggagga tttcggtctc gcgctcggag agcggcgtcg ccggcagctc
     3721 gtcacgctgt ggcagatggc ccggtggaaa cggcgggagc gaatcgatca ggtgaagctg
     3781 ctcgcgaatc gaaccgaaat tctcgcgaag gtacgtccgc gtcatcaccg agcgattggc
     3841 gttggcatcc tgccattcgc ggcgttgcac gtgaatcgtc gcgttgggaa acgtcgaccg
     3901 ggcgtcgggc gcacgggtca atccgccggc gtggtcgaag tgcaggtggc tgacgatcac
     3961 gtgatcgatc gcttcgcagc tgccgccggc atcgcgcacg gcgtcgatga tcgagtagtc
     4021 gccgaggccg aagatctttc ggttcttctc gtcgaactta tcgccgctgc cgacttcgat
     4081 caggatgcgc ttcggctcgc gggcatccgg cgtcgcgggc tcgagcagca ggcaattgtg
     4141 cgcgcattcg atccggccca tctcgtcggc aggggcgacg cggctccaga ccgcgcgggg
     4201 gacgacgccg aacatgctgc cgccgtcgag gcgcagcgga ccggcgcgca gcagttgcca
     4261 tttgtatcgt ggctcactcg ccatagtgtt ctcgatcctt cactcgtagc tgcgacgcct
     4321 gcgtcgccgt cgctcgaaaa aaaggcgacg ccggcgtcgc ggctacgttg gaagcagttc
     4381 atccggcgtc tgcagcaggc tgacgatctt acccagcgcc gccgcggcgg tgccgccgtc
     4441 gatcacgcga tggtccgcgc tcaagctgag cggcagcagc ttgccgacgc caagcatgcc
     4501 gtcgcggacg acgacgcctt cgcgcgcccg gccgatcgcc aggatggcgg cttcgggata
     4561 gttgatgatc ggcgtgccga atcgcccgcc catcgcgccg aagttgctga tcgtgaacgt
     4621 cgatccggtt agttgatcgg gcgacaattc gcggccgcgc gcggcttgcg ccagctgcgc
     4681 gatcatgcgg ctcaattcca aaacgccaat gtgctccgcg tcgcgaatga ccggcaccat
     4741 caatccgctt tcggtgtcgg tggcgatgcc caggtgcacc gcgcggtgtt gcacgatctc
     4801 ctggtgttcg tcatcgaccg tcgagttgag ccgagtgaac ttggcgccgg agcgaccaga
     4861 cagaacgcgg cagatggcga cggcgacgaa gggcagaagg ctgatcttct cgttcgacgc
     4921 cgcgaccagc ttgcggcgca gcgcgtcgag ggccgtcacg tccgcctcgt ccatcacggt
     4981 gaagtgaacg gcgtgggtga tgctgtggtg caggtgctcg gcgatcgttc ggcgaacgcc
     5041 gcggaagggc gtgcgcagcg atgcggcggg gttggccggt tgcttcgcag ccggtttgcg
     5101 tttgtgcgcc gcgggttgat cctgctcggc gacggcggcc tgcacgtcgc cggccgtgat
     5161 tcgtccgccg atgccggtgc cgtgcacgtg cgagagctcg atgcccatgt cgcgcgccaa
     5221 gcgccgcacc gccggggcgg cgaggctttt gccttcggct tcgatcgcgg tggcgtcgcc
     5281 caaatcgccg acgacggatc ccgcgtcgcg gcgttcttcg acggccggcg cgttgcgcgg
     5341 cgcaccgttg ccggagctgg acacgtcgcc tttgaacgtc accagcggcg cgccaacttt
     5401 aatcgcctgc ccgggtttgc cgtgcagcat tgcgataatt cccccgcgag ggctgggcac
     5461 gtccacgagc gctttagcag tttccatttt cg
//
LOCUS       DATIMH010000579         7465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_719405, whole genome shotgun sequence.
ACCESSION   DATIMH010000579 DATIMH010000000
VERSION     DATIMH010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719405"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1320)
                     /locus_tag="VLI90_10030"
     CDS             complement(25..1320)
                     /locus_tag="VLI90_10030"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HSV14589.1"
                     /translation="MLSRKRLVILLMAILSMRASAQTTAPALTPQQQWWELQRQPAGP
                     QAVVRVHDPCAIFANNAYYLFCTGRGTPMFRSDDRYHWTRVGVVFPQQPDWALREVPG
                     SRGQWAPDISLFNGVYHLYYAVSTFGSRRSCIGLTINKTLDVNSPDFRWIDQGKVIET
                     HNSDNYNAIDPNLVMDEHDQPWMVFGSCWDGVRISRLDVATGKPPSDEPPPRPLEHRP
                     KGKLFEGPFVVRHGEWFYLFISFDACCRGAQSTYNIRVGRSRHVDGPYVDMDGVPMLE
                     GGGTLVLGSYGSVRGPGHCGVMTDAAGRQWMVHHFYDADDRGISKLQVRRIVWSKDEW
                     PLVGEPVDRPVSGQVNRKDVAGFWKYSVNFGDDRPVRLKADGSSDAPDVISSWRIEDG
                     RLALNVPTGRAMCEIADDGEWFVGRDQSGVILRGHRSRN"
     gene            complement(1663..5652)
                     /gene="secA"
                     /locus_tag="VLI90_10035"
     CDS             complement(1663..5652)
                     /gene="secA"
                     /locus_tag="VLI90_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSV14590.1"
                     /translation="MPVTFGKVLTKVFGSRNERLIKRYRRIVEQVNAMEGKIAALTDE
                     QLRARTQELRAGLTSGSLKSAAVMPEAFAIVRESMDRHIGIRQIFNPEENFDPDRFDD
                     EHLELYDSVQRRMINEGMSWQRVPIPVKIYDAVRKMYPDSRPPYRARCFDVQLIGGLV
                     LYEGKIAEMATGEGKTFVAPLACFMRVLEGFHCHVVTVNDYLVRRDASWVAPAFENLG
                     MSVGFLQQDMEPGGETRRRQYGADITYGTNSEFGFDYLRDNMKERVELQVQGPLDFAI
                     VDEVDSVLIDEARTPLIISGGAHDDAPKYRAADAVARAVIEKAKPWDAVEKQVDAAKR
                     AIKSAQGDADKARTKEEKEAARKREEEAAKQLVEAEAKKEGLTQYYEVELDRKSVHLT
                     HEGIAAAQEAAGVGSFYVGNNMEWPHLMEQALRAHVVYERDKDYVVEKGQKGEMEVVI
                     VDEFTGRKMVGRQWSDGLHQAVEAKERVPIKQETQTLATITLQNFFKLYRALSGMTGT
                     AMTEAEELSKIYRLDVVTIPTNRPVIRQDNEDRVYRTEQEKWMAILDEIRDMSDTGRP
                     VLVGTTSVEKSEMLSTLLKRKYGVEHEVLNAKNHEREAQIVALAGQQHRNAHGELVGN
                     VTIATNMAGRGTDIKPAQEAFFDIIETPQGKTGGKWVVKQRNTGKLIEVESKMGENGE
                     ENPLVNLLQLTGGSKVVGGLHVVGTERHTARRIDNQLRGRSGRQGDPGSSRFYVSLQD
                     DLMAMFAGEWTIKVLGWLGMEEGMAIEDKRISKGIVRAQKKVEERNYLARKNLLEYDD
                     VMDKQRTTFYGMRQQVLEGRDVAGVIWSMIGEAINDAVDKYITQDYVASSVSEWARTN
                     FEVQIDANDIKVRRDFHFLEHYVKDQARAEANTTISSTVGEFMGEDESDTSTWDAKGL
                     SSWAMSRFHVQLSQNQIRKSTPEEIDTQLRTAAVEQIDKRDCAGLEKYLQPLYAENEL
                     AGWAREKFGVEMTGQEIADAVKHTRNTPAEAIAELLEERARAAYARRDIEYPVTQALL
                     YAFNDENGSTDNPYAADWVRLWAKSKYGVDIPVEHIRSSTVRELHEELCRLQEEFMRT
                     NRIERDIDAIMAVGTTPKERLIAFNERFGLNLKENIFKPDQLPPAVRAIIGDGNKNGD
                     GRAAAEVEPREVMLRLAKAHLRRELTDLEQFVLIQIFDTSWKDHLYAMDMLKGGIGLQ
                     SFAEQDPRVLYKLQGFEYFEQMMAGIRDKVTDLIFRARVMGKVESRSNYRETAAVHAD
                     TGGYGVTENIAATASAVESHASPEGGDGEAATAVKTIVREEPKVGRNDPCPCGSGKKY
                     KKCCGANAA"
     gene            complement(5824..6120)
                     /locus_tag="VLI90_10040"
     CDS             complement(5824..6120)
                     /locus_tag="VLI90_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14591.1"
                     /translation="MRRARYLLVATLLLTAVCADRAVAAAPALRPEMGQLAERLVNRL
                     SASFSRTVSAVRMVAERQQAGAFAADRSLPISWVAPAHPQPVSPFQFRLPPPAN"
     gene            6410..6913
                     /locus_tag="VLI90_10045"
     CDS             6410..6913
                     /locus_tag="VLI90_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14592.1"
                     /translation="MARPIPVWPRGPRRLRRRSLQVLLACPRVSPEWELAVVDALMRP
                     KVLTKGKVEHRRGEQAWPVDAETRAREIVQEVSRRLDAAFDIAYPPEGEYLLVGEAPR
                     GYEEETKALAAALSSALPEQWFVLGRLFVKSGRFYRRRFGYRMDVVPATDVHLPRAMR
                     AKLKGLI"
     gene            7157..>7465
                     /locus_tag="VLI90_10050"
     CDS             7157..>7465
                     /locus_tag="VLI90_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HSV14593.1"
                     /translation="MTNVPMIRETISTRSRNEMIDITKRVQQAASDAQVTNGIVIVYV
                     PHTTAAVTINENADPDVQHDLLEKLGKLVPQSEAFYEHGEGNSDAHVKAACVGSSVTV
                     L"
BASE COUNT         1259 a         2475 c         2229 g         1502 t
ORIGIN      
        1 cgcccgagct acgggacacg ctcttcaatt cctcgatcga tgcccgcgca ggatcacgcc
       61 agattgatct cgcccgacaa accactcgcc atcgtcagcg atctcgcaca tcgcccttcc
      121 ggtcggaacg ttcagcgcca accgcccgtc ctcgatccgc caactcgaaa tgacatcggg
      181 cgcatcgctc gacccatccg ccttcagccg caccgggcga tcgtcgccga agttgaccga
      241 gtacttccaa aaccccgcca cgtccttgcg attgacctga ccagacaccg gccggtccac
      301 cggttcaccc accagtggcc actcatcctt cgaccaaacg atccgccgca cctgcagctt
      361 tgagatgccg cggtcatctg cgtcatagaa atgatgcacc atccactgcc gccccgcggc
      421 gtcggtcatc acgccgcaat gcccggggcc gcggacgctt ccgtagctgc ccagcaccag
      481 cgtcccgccg ccttcgagca tcggcacgcc gtccatgtcc acatacggcc catcgacgtg
      541 ccgcgatcgg ccgacgcgga tgttgtacgt gctctgcgcc ccgcggcagc aggcgtcgaa
      601 ggagatgaac agataaaacc attcgccgtg gcgaacgacg aacgggcctt cgaacagctt
      661 gccctttggc cgatgttcca gcggccgcgg cggcggttca tcactgggcg gtttgcccgt
      721 ggcaacatcg agccggctga tgcgcacgcc gtcccagcac gagccgaaga ccatccacgg
      781 ctggtcgtgc tcgtccatca ccaaattcgg atcgatcgca ttgtagttgt cgctgttgtg
      841 cgtctcgatc accttgccct ggtcgatcca gcgaaagtcc gggctgttta catcgagcgt
      901 tttgttgatt gtaaggccga tgcacgaccg ccggctgccg aacgtggaga cggcgtagta
      961 caggtgatag accccgttga acagcgagat gtccggcgcc cactgcccgc gcgagccggg
     1021 cacttcgcgc agcgcccaat ccggctgctg cgggaagacg acgccgacgc gcgtccagtg
     1081 ataccggtcg tccgaccgaa acatcggcgt cccgcggccg gtgcagaaca ggtaatacgc
     1141 gttgttggcg aagatggcgc acgggtcgtg cacgcgcacg accgcctgcg gccccgccgg
     1201 ttggcgctgc agttcccacc attgctgctg cggcgtcagc gccggcgccg tggtctgcgc
     1261 cgatgcgcgc atcgacagga tcgccatgag caaaatgacc agccgcttgc ggcttagcat
     1321 gggtttaatc gtccctcgcg aaaaggatgc taagccgcaa gcggctcatt tcctcgacaa
     1381 caccacggtt gccgcgctca tccattcgtg ggcgcgacgt tcatcgaaca ctcgccggtg
     1441 ctcgcatcga atgcaacgtc tcccgccgtt ccaccgtcca gcggcgaaca acgtcgcgac
     1501 taagagttgc gccggatgca actctttccc cccctcccgg tactccggga gagggtcggg
     1561 gtgagggtga tttcgaggac cagcggctat cgaagaacag aatcaccctc accctctccc
     1621 ggcgacctgc agtcagcagt accgggcgag gggaccggag ccctacgccg cgttcgcgcc
     1681 gcagcacttc ttgtacttct tcccactgcc gcaggggcag gggtcattgc ggccgacctt
     1741 gggctcttcg cggacgatcg tcttcaccgc cgtggcggcc tcgccgtcac cgccttcggg
     1801 tgacgcgtgc gattcgaccg ccgacgcggt ggccgcgatg ttttcggtca cgccatatcc
     1861 accggtatcc gcgtgtaccg cggcggtttc gcggtagttg ctgcgcgatt ccaccttgcc
     1921 catcacgcgg gcgcggaaga tcaggtcggt gaccttgtcg cgaatgccgg ccatcatctg
     1981 ctcgaagtat tcgaagccct gcagcttgta gagcacgcgc gggtcctgct cggcaaaact
     2041 ttgcagcccg atgccgccct tgagcatgtc catcgcgtac aggtggtctt tccagctggt
     2101 gtcgaagatc tggatgagca cgaactgctc caggtccgtc agctcgcggc gcaggtgcgc
     2161 cttggccagc cgaagcatca cctcgcgcgg ctccacttcc gccgccgcgc ggccgtcgcc
     2221 gttcttgttg ccgtcaccga tgatcgcccg caccgccggg ggcaactggt cgggcttgaa
     2281 gatgttctcc ttgaggttca acccgaagcg ctcgttgaac gcgatgagcc gctctttcgg
     2341 cgtcgtcccc accgccatga tcgcgtcgat gtcgcgctcg atgcggttgg tccgcatgaa
     2401 ctcttcctgc agccggcaca gctcttcgtg caattcgcgc acggtgctgc tgcggatgtg
     2461 ctcgacggga atatcgacgc cgtacttgct cttggcccac agccgaaccc agtcggccgc
     2521 gtacggatta tccgtgctgc cgttttcgtc gttgaacgcg tacagcagcg cctgcgtgac
     2581 cggatactcg atatcacgcc gcgcgtacgc ggcccgtgcg cgttcttcca gcaattcggc
     2641 aatcgcttcc gccggggtgt tgcgggtgtg cttcaccgcg tcggcgattt cctgacccgt
     2701 catctccaca ccgaacttct cgcgcgccca tccggccagt tcgttctcgg cgtacagcgg
     2761 ctgtagatac ttctccagac ccgcgcaatc gcgcttgtcg atctgctcca ccgccgccgt
     2821 gcgcagctgc gtgtcgatct cttccggcgt gctcttgcga atctggttct ggctcagctg
     2881 cacgtgaaac cggctcatcg cccagctgct caggcccttg gcgtcccacg tgctcgtgtc
     2941 gctttcatct tcgcccatga actcgccgac cgtcgagctg atcgtcgtat ttgcctcggc
     3001 ccgcgcctgg tccttgacgt aatgctccaa gaaatggaag tcgcgccgga ccttgatgtc
     3061 gttggcgtcg atctgcactt caaaattcgt gcgcgcccat tcgctgacgc tcgatgcgac
     3121 gtaatcctgc gtgatatatt tatcgaccgc gtcgttgatc gcttcgccga tcatgctcca
     3181 gatcacaccg gccacgtcgc ggccttccaa cacctgctga cgcatgccgt aaaacgtggt
     3241 gcgctgcttg tccatcacgt cgtcgtattc gagcaggttt ttgcgcgcca ggtaatttcg
     3301 ctcttcgacc ttcttctgcg cgcgaacgat gcccttggag atgcgcttgt cctcgatcgc
     3361 catcccctct tccatgccca gccagcccag gaccttgatc gtccattcgc cggcgaacat
     3421 cgccatcaag tcgtcctgca gcgaaacata gaagcgcgac gaccccggat cgccctgccg
     3481 gcccgatcgg ccgcgcagct ggttgtcgat gcgccgcgcg gtgtgccgct cggtgccgac
     3541 gacgtgcaat ccgccgacca ccttcgatcc gccggtcagc tgaagcaggt tgaccagcgg
     3601 gttttcctcg ccgttctcgc ccatcttcga ttcgacttcg atcagcttgc cggtattacg
     3661 ctgcttgacg acccacttcc cgccggtctt gccctgcggg gtttcgatga tgtcgaagaa
     3721 cgcctcctgc gccggtttga tatcggtccc gcgaccggcc atgttggtgg cgatcgtcac
     3781 gttgcccacc agttcgccgt gcgcattgcg atgctgctgc ccggccagcg cgacgatctg
     3841 tgcctcgcgc tcgtggttct tggcgttcag cacttcgtgc tcgacgccgt acttccgctt
     3901 cagcagcgtc gagagcatct cgctcttttc cacgctggtc gtgccgacga gcaccggccg
     3961 gccggtgtcg ctcatgtcgc gaatttcatc caggatcgcc atccactttt cctgctcggt
     4021 gcggtagacg cgatcctcgt tgtcctgccg gatgaccggg cggttggtgg gaatcgtcac
     4081 gacgtccagg cgatagatct tcgacagctc ttctgcttcg gtcatcgccg tgccggtcat
     4141 gccggacagc gcgcggtaca gcttgaagaa gttctgcagc gtgatcgtcg ccagcgtctg
     4201 cgtttcctgc ttgatcggca cgcgctcttt cgcttccacc gcctggtgca ggccgtcgga
     4261 ccactgccgg ccgaccatct tgcgaccggt gaattcgtcg acgatgacga cttccatctc
     4321 ccccttttgg cccttctcga cgacgtaatc cttatcgcgc tcgtacacca catgcgcgcg
     4381 cagcgcctgc tccatcaggt gcggccactc catgttgttg ccgacataga acgacccaac
     4441 cccggccgcc tcctgagcgg cggcgatgcc ttcgtgcgtg aggtggaccg acttccgatc
     4501 caattcgact tcgtaatact gcgtgagccc ttccttcttc gcttccgcct cgaccagctg
     4561 ttttgccgcc tcttcctcgc gcttgcgcgc ggcttccttc tcctctttgg tccgggcttt
     4621 gtccgcgtcg ccctgggcgg atttgatcgc gcgcttcgcc gcgtccacct gcttctccac
     4681 cgcatcccac ggcttcgcct tctcgatgac ggcccgcgcg accgcgtcgg ccgcgcgata
     4741 tttcggcgcg tcgtcatgcg ccccgccgct gatgatcagc ggcgtgcgcg cctcgtcgat
     4801 cagcacgctg tccacctcgt caacgatcgc gaaatccagc ggcccctgca cctgcagctc
     4861 gacccgctcc ttcatgttgt cgcgcaggta gtcgaaaccg aattcgctgt tggtgccgta
     4921 ggtgatgtcc gcgccgtatt gacgccgccg cgtttcaccg ccgggttcca tgtcctgctg
     4981 caggaatccc accgacatgc ccaggttttc aaacgccggc gccacccagc tggcgtcgcg
     5041 gcggaccagg tagtcgttca ccgtcacgac gtgacagtgg aacccctcga gcacgcgcat
     5101 gaagcacgcc agcggcgcga cgaacgtctt gccttcgccc gtcgccatct cggcaatctt
     5161 gccttcgtac agcaccagcc cgccgatcag ctgcacgtca aagcagcgcg cccgatacgg
     5221 cggccggctg tcggggtaca tctttcgaac cgcgtcgtaa atcttcacgg ggatcggcac
     5281 gcgctgccag ctcatccctt cgttgatcat ccgccgctgc accgagtcat acagctccag
     5341 gtgttcgtcg tcgaaccgat cgggatcgaa attttcttcc gggttgaaga tctgccggat
     5401 gccgatgtgc cggtccatcg attcgcggac gatcgcgaac gcctccggca tcaccgccgc
     5461 gctcttcaac gatccgctgg taagcccggc gcgcagctcc tgcgtgcggg cgcgcagctg
     5521 ctcgtccgtc agggccgcga tcttgccttc catcgcgttg acctgttcga cgatccttcg
     5581 ataacgcttg atcagccgct cgttgcggct gccgaaaact ttcgtgagca ctttgccgaa
     5641 tgtgacaggc atgggaacct ctcgggggag aataagttgc tggttgtatg ttgctagttg
     5701 ctagtgaaag acggatagca gagcgaggcg cgcgcaaaca tacgtgacgc gcgcgcgagg
     5761 gaggggctga tcgcaagtcg ctttagttgc atttacgagc aacgagcaac tagcaaccag
     5821 taactaattc gccggaggcg gcagacggaa ctggaaggga gagacgggct gcggatgggc
     5881 cggggcgacc catgagatcg gcagcgaacg atcggcagcg aaggcgccgg cctgctgtcg
     5941 ttcagccacc atccgaaccg ccgacacggt ccggctgaag ctggcgctaa ggcggttgac
     6001 caaccgctcc gccaactgcc ccatctccgg ccgcagcgcc ggcgcggcgg ccacggcgcg
     6061 gtccgcgcac acggccgtca gcagcaacgt cgcaaccaga agatagcggg ctctacgcat
     6121 cgcggtacta cttcttcgac cggtactact tcttcgacag gaatcgagcc acacagtatg
     6181 acccaacgcg cgcggcgttt caacgcccgc gccgatccgc gcgataccga tgatacgccc
     6241 gcggatggca catggtttca tgaaagctgc gttggatgcc ctgcgtcgaa tgccgaatcg
     6301 agtcgcgccg aagcccgcgc attatcttcc gtcctgcgcc catcattccg caccgtcgcc
     6361 aatccaccgc cgcgatgatc ggcacaatcg gacctatgat ctgttggata tggcccgtcc
     6421 gattccagtt tggcctcgcg ggccgcggcg gttgcggcgg cggtctttgc aggtgctgct
     6481 ggcgtgcccg cgggtgtcgc cggagtggga gttggcggtg gttgacgccc tgatgcggcc
     6541 gaaggttttg accaagggga aggtcgagca tcggcggggc gagcaggcgt ggccggtgga
     6601 tgccgagaca cgggcgaggg agatcgtcca ggaagtctca cggcggttgg acgcggcgtt
     6661 cgacattgcg tatccgcccg agggtgaata tttattggtg ggcgaagccc cgcgggggta
     6721 tgaagaggaa actaaggcgc tggcggcggc gttgtcgagc gcgttgccgg aacaatggtt
     6781 cgtgctcggc cgcctgttcg tcaaaagcgg acggttttat cgccggcggt tcgggtatcg
     6841 aatggacgtg gtgccggcga cggacgtgca cctgccgcgg gcgatgcggg cgaagttgaa
     6901 ggggttgatc taagtacggc gtgcgcgaag gtgtaaccac ggagacacgg agacacggag
     6961 aagaaacaga ggaggaacca cagatgcaca cagatgaaca cagataaaag aagaaattac
     7021 gatctgtctt atctgtgttc atctgtgtgc atctgtggtt cctacctctt cgtctctgcc
     7081 tccgtggtga atcattctgt tgggagttcc aagatctaga agcgcggcgt gaatggcgcg
     7141 ctgttgagga aacgacatga cgaacgtgcc gatgattcgc gagacgattt cgacgcgcag
     7201 ccgaaacgaa atgatcgaca tcaccaagcg cgtgcagcag gcggccagtg acgctcaggt
     7261 gaccaacgga atcgtgatcg tatacgtccc gcacacgacg gcggcggtga cgatcaacga
     7321 gaacgcggac ccggacgtgc agcatgacct gctggagaag ctcggcaagc tggtgccgca
     7381 gagcgaggcg ttttatgagc atggcgaggg gaacagcgac gcacatgtga aggcggcgtg
     7441 cgtcgggtcg agcgtgactg tgcta
//
LOCUS       DATIMH010000580         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3890794, whole genome shotgun sequence.
ACCESSION   DATIMH010000580 DATIMH010000000
VERSION     DATIMH010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890794"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1457
                     /locus_tag="VLI90_10055"
     CDS             171..1457
                     /locus_tag="VLI90_10055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV14594.1"
                     /translation="MLIPLGSTMIAVALPSIGTEFGRAPAELTTWLVTSYLLVSVIGQ
                     GPGGKIGDYWGYRRTIFLGQCIFGVGSLLGLLDFFAAVVASRVLMALGGAFMVPTVMA
                     VFKITVPAALRHRVFGYFGAMMTLAAAVGPMLGGVLVHQFGWKSIFLMNLPPLILSAL
                     LSKKFFSEHLHEPPTAAFRYDWQGSVLMTCGLTCLVLAFKNRMELMVPAVGFLAAFLW
                     WEGRTAQPLINLKLFSNRFFSAGCAMVALQNLAMYALLFQLPFLLKLLYGWGPERAGP
                     FMTAFMVSMMTASALGGRVAERAGVRATCVMGSIISVAGLYWLSTLAPGEESMHVIWG
                     LVFGGAGLGLANGPSQSAAMSTVDNRQSGIASGVLSTCRYLGGIIGVSILGLLLSTGD
                     AAPTLGDYHMALMIFTGAFVIAASVALRLPGHARPV"
     gene            1639..1875
                     /locus_tag="VLI90_10060"
     CDS             1639..1875
                     /locus_tag="VLI90_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HSV14595.1"
                     /translation="MPHVTVKMWPGPSEQQKRRLADQITKDVVTLLDSDDASVSVAIE
                     EVNPGDWLESVYRREIEPRMDDLYKRPGYGPSDL"
     gene            1893..>2332
                     /locus_tag="VLI90_10065"
     CDS             1893..>2332
                     /locus_tag="VLI90_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSV14596.1"
                     /translation="MSVFNPKRRKLLQAGAAAGAAAATPLVPTEVEAATAQASKFPSV
                     ALMNVRLRVNGTEHTVMLDPRTSLLDAVRERLHLTGAKKGCDQGQCGACTMIVDGRRI
                     NACLTLAVMHQGAEITTIEGLGQPDNLHPMQAAFVKHDGYQCGY"
BASE COUNT          390 a          728 c          736 g          478 t
ORIGIN      
        1 gctcgtgcgc ctcgcgcacc tgtgcgagaa acactttggt cccggccgcg acatcgagtg
       61 ggggatcgag aacgacatcc tctacctgtt gcagtgccgc tccattacga cggcctaggc
      121 gcttgtccga ctattcgccc ctgaggctcg cagggctggg cctctcggcc atgctgatcc
      181 ctctcggctc cacgatgatc gccgtggcgc tgccctccat cggaacggag ttcgggcgcg
      241 cacccgcgga gctcacgacg tggctcgtga cgagttacct cctggtgagc gtcattggcc
      301 agggtcccgg gggaaagatc ggggattact ggggctacag gagaaccatt tttctcgggc
      361 aatgcatatt cggagtcgga tccctgctcg ggctgcttga tttctttgcc gctgtcgtcg
      421 cgagccgcgt gctgatggcc ctgggcgggg cgttcatggt gccgaccgtg atggcggtct
      481 tcaagatcac cgtgcccgcg gcattgcgcc accgcgtctt cggctatttc ggcgcgatga
      541 tgacgctcgc ggccgcggtt ggcccgatgc tcggcggcgt gctcgtccac cagttcggct
      601 ggaaatcaat cttcctgatg aacctgccac cgctcatcct ctcggcgttg ctctcgaaga
      661 aattcttcag cgagcacctt catgaaccgc cgactgccgc attccgctac gactggcagg
      721 gcagcgtgct gatgacctgc ggcctgacgt gcctggtcct ggcattcaag aaccgcatgg
      781 agctgatggt gccggccgtc ggcttccttg ccgcgtttct ttggtgggaa ggacgtactg
      841 cgcagccgct catcaacctc aaactctttt cgaatcgatt cttttccgcc ggctgcgcga
      901 tggtggcctt gcagaatctc gccatgtacg cgctcctttt tcaattgccg ttcctgctga
      961 agctgctgta cggatgggga ccggagcgcg cggggccatt catgacggct ttcatggtga
     1021 gcatgatgac cgcctcggcg ttgggcgggc gcgtcgccga acgcgcgggc gtgcgcgcta
     1081 cgtgcgtgat gggctccatc atttccgtcg ccggtctcta ctggctttcg accctcgccc
     1141 ccggcgagga atcgatgcac gttatctggg gcctcgtttt cggtggcgcg ggtctcggtc
     1201 tcgcgaacgg gccgtcccag tcggcggcca tgtccacagt cgacaatcgc cagagcggaa
     1261 ttgcttccgg tgttctctcg acctgtcgtt atctcggggg aatcatcggc gtttcgatcc
     1321 tgggactgct gctctcaacg ggtgacgctg cgccgaccct tggggattac cacatggcac
     1381 tgatgatctt cacgggggcg ttcgtcatcg ccgcctctgt ggctttgcgg ctgcccgggc
     1441 atgccaggcc ggtgtgacgg taagagtacg gaatcaccgc atcggcacgc attagcagcg
     1501 attgcgggat tgtgatcctg agcgcctgag tagcttcgac agatggacta cgcgccctgc
     1561 tcgcgggtgt tgaaccgcag cagcgggcga gactcctatc aacgtccacc tcatctctta
     1621 gtgcggagag gagaaaacat gccccacgtg accgtgaaga tgtggcctgg cccatccgag
     1681 cagcagaagc gccgtctcgc ggatcagatc acgaaggatg tcgtgaccct gctggactcc
     1741 gacgacgcgt cggtgtcagt ggcgatagag gaggtgaatc cgggggactg gcttgaatcg
     1801 gtctaccgtc gcgagatcga gccgcgcatg gacgatctct acaaaaggcc tggctacgga
     1861 ccttcggatc tatgaatgga aaggacctcc ccatgagcgt tttcaaccct aagcggcgca
     1921 agctgcttca agccggcgct gcggcgggcg cggcagcggc aacgccgctt gtcccgaccg
     1981 aagtagaggc tgcgacggcg caagcaagta aattcccctc cgttgcgctg atgaacgtcc
     2041 gccttcgcgt aaacggtacg gaacacaccg tcatgctgga tccccgcacg tcactcctcg
     2101 atgcggtccg tgagcgactg caccttacgg gcgcgaaaaa aggctgcgac caggggcagt
     2161 gcggcgcctg cacgatgatc gtcgacgggc ggcgcatcaa tgcctgcctg acgctcgccg
     2221 tcatgcacca gggcgccgag atcaccacga tcgaagggct cgggcagccg gacaacctgc
     2281 acccgatgca ggccgccttc gtgaaacatg acggctacca gtgcggctac tg
//
LOCUS       DATIMH010000581         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_428859, whole genome shotgun sequence.
ACCESSION   DATIMH010000581 DATIMH010000000
VERSION     DATIMH010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428859"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VLI90_10070"
     CDS             complement(<1..840)
                     /locus_tag="VLI90_10070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSV14597.1"
                     /translation="MSIQPLADAAQTLVPADPATPAAAAPTTQPQFSPALDVCVLAWF
                     AGAVLVAKMLRVFRRQSIVGPRRLAEGDSALWLVAALFAAYFFGVFAGGVVLRFVHVR
                     EDVQPLVLTVIVGGVGVPIILAALRVMNPRAIQSMGFSPRRILPGALGAVVALPIIYP
                     AVVVASDATVLLIHWLRLPEPHTHEVLQSLGSTHDRRMIALDLLTAVVIAPIFEELIF
                     RGLVQTALSRMFTWLLGPRALARWAAVIITAGAFAAVHGQWAFVPPLMVLAIALGYAY
                     ERWG"
     gene            959..1798
                     /gene="trpC"
                     /locus_tag="VLI90_10075"
     CDS             959..1798
                     /gene="trpC"
                     /locus_tag="VLI90_10075"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001373.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HSV14598.1"
                     /translation="MSGDSSGNILDRIIETKRQEVADRQQRVPIEQLKDTITTLGRPR
                     NFFQAVSKDPKTGKPPKPLNLIAEVKKASPSAGVIRANFDPVKIAQQYAAAGADALSV
                     LTDEQYFQGHLDYIHAIRDVVKLPVLRKDFIIDPYQVYESRAAGADAILLIAECLETS
                     EMIDLQILATELNLTCLIEVHDLDNLMRVRDHVIGFPHRSYSLLGINNRDLRTFKTDL
                     GTTLRVTELVEDRSVLVSESGINSRADVKKLADAGVRAVLVGESLMRSDDIAAKIREM
                     FLS"
BASE COUNT          424 a          702 c          663 g          326 t
ORIGIN      
        1 tccccagcgc tcgtacgcgt accccagcgc gatcgccagc accatcagcg gcggcacaaa
       61 cgcccactgc ccgtgcaccg ccgcgaatgc tccggcggtg atgatcaccg ccgcccagcg
      121 cgccagtgcc cgcgggccca gcagccacgt gaacatccgc gacagcgccg tctgaaccag
      181 cccgcggaag atcagctctt cgaagatcgg cgcgatcacg accgccgtca gcaggtccag
      241 cgcgatcatc cgccggtcgt gcgtgctgcc cagcgactgc agcacctcgt gcgtgtgcgg
      301 ctccggcagg cgcagccaat ggatcaacag caccgtcgca tccgacgcga cgaccaccgc
      361 agggtaaatg atcggcagcg ccaccaccgc gcccagcgcc cccgggagaa ttcgccgtgg
      421 cgaaaatccc atcgactgga tcgcccgcgg gttcatcacg cgcagcgctg ccaggatgat
      481 cggcacaccc acgccgccga cgatgaccgt cagcaccagc ggttggacgt cctcgcggac
      541 gtggacgaag cgcagcacca caccgcccgc gaagacgccg aagaagtacg ccgcgaacaa
      601 tgcggcgacg agccacagcg ccgagtcgcc ctccgccaat cgccgcggtc ccacgatcga
      661 ctgccggcgg aaaacgcgaa gcatcttggc aaccagcacg gccccggcga accaggcgag
      721 gacacataca tcgagcgcgg gtgagaattg cggttgggta gtcggcgccg ccgccgcggg
      781 cgtcgctggg tctgctggca cgagcgtctg ggcggcgtcc gccaggggct gaattgacat
      841 tgaatcgatc cgatttacag tgccccccgc gcgcagccgg cctatcgttc acagcgcacg
      901 cgcgcgtcgc tgctcgacat gggcggcggc attttagacg gcggtgacac acttgcctat
      961 gagcggcgac agcagcggga acatcctcga tcgcatcatc gaaaccaagc gccaggaagt
     1021 ggccgaccgg cagcagcgcg tgcctatcga acagctgaag gacacgatca cgacgctggg
     1081 tcggccgcgg aatttcttcc aggcggtcag caaggatccc aagaccggca aaccgcccaa
     1141 gccgttgaac ctcatcgccg aggtgaagaa ggcatcgccc agtgccggcg tgatccgcgc
     1201 gaatttcgat ccggtgaaga tcgcccagca atacgccgcc gccggcgctg atgcgctgag
     1261 cgtgctgacg gacgagcagt atttccaggg ccatctcgat tacatccacg cgatccgcga
     1321 cgtggtgaag ctgccggtgc tgcggaaaga ttttatcatc gatccgtacc aggtgtacga
     1381 aagccgggcc gccggggcgg acgcgattct gctgatcgcg gagtgtctcg agaccagcga
     1441 gatgatcgac ctgcagattc tggcgacgga gctgaacctg acctgcctga tcgaggtgca
     1501 cgacctggac aacctgatgc gtgtgcgcga ccacgtcatc ggcttcccgc accgcagcta
     1561 tagcctgctc ggcatcaaca accgcgacct gcggacgttc aagacggacc tgggtacgac
     1621 gctgcgtgtg acggagttgg tggaagatcg atcggtcctg gtcagcgaaa gcggcatcaa
     1681 cagccgcgcg gatgtgaaga agctggcaga tgcgggcgtc cgcgccgtat tggttggtga
     1741 aagcttgatg cgcagcgacg acatcgccgc caagatccgc gagatgttcc tgagctgatg
     1801 caatcccccc tgatagccgc aggcgacagc cagcgggatt tttccaaatc aaaccggctt
     1861 cgctctccaa agcccccgct ggctgtcgcc tgcggctatt aaaggttcat caacgggcag
     1921 cgatcgaacg gctgccgctg ccaaatctcg cgtttgttgt ccaattcttc cccgcgcagt
     1981 aaaacccttg cgggcggcgc gcgatcaatt tgtgtcagag ggagtcttct tgaataagca
     2041 agcgctaatc gtctggggtg gctgggacgg gcacgagccc cggcaggtcg cggagatctt
     2101 ccgccgcgta ttggc
//
LOCUS       DATIMH010000582         3499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2160021, whole genome shotgun sequence.
ACCESSION   DATIMH010000582 DATIMH010000000
VERSION     DATIMH010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160021"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VLI90_10080"
     CDS             <1..1030
                     /locus_tag="VLI90_10080"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14599.1"
                     /translation="DPKIKGVNLAVYSTVPLGAGVSSSAAIEVATMMNLVEHFSIRAA
                     VDPLRMAAMCQAVENRIVGAPCGIMDQVSSGYGQAGALLRMTCQPHKVESPLHLPPGT
                     RVIGINSNVKHSVGGGMYGRTRCAAFMGHQIILTKMREMGAAAGKALVGDPLNGYLAN
                     LDQDDYKQLFRPHLPEQIGGQDFLDRFGPTIDQATKVDPTQSYHVQQATDHHVLEARR
                     VQQFVEFLQQAAKCQGLPRKNLLDKAGHLMYASHLSYTNDALLGAEECDLLVQLVRDR
                     EPAGLYGAKITGGGSGGTVAVLAEKSPSVDAAVNQIMSIYEQRTGRKAEAFLGSSPGA
                     WHVGTAVV"
     gene            complement(1095..2033)
                     /locus_tag="VLI90_10085"
     CDS             complement(1095..2033)
                     /locus_tag="VLI90_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14600.1"
                     /translation="MAKGKHATALFEVIHTSRRPPKASPSGGIPTPKWWFKSKRSSLD
                     ALFSSRSEAPATPAPVVEPPPRIIERIIEKPVYIERPAPPLRIGGDSPVGVENGNGEL
                     RFKLSYGGAAAAGFVLVLIVAFAYLAGTRTTTYDEYRPAVGASGNGVAHGQTTSAGFL
                     AAVTTPPANPAVMNVGRAAPTAAVKAPASAPSAAAVTDGKGPRQVGLHYIIAQSYPDK
                     EIAKRACDFLNKAGIPCTVVSGPAGWAQRSWYSVVTVKSFDHIQRNPELEQLKEEIAT
                     ASTRFAGAKSFYRFEPKPYKWRGDADPQAASADVEQ"
     gene            complement(2307..>3499)
                     /locus_tag="VLI90_10090"
     CDS             complement(2307..>3499)
                     /locus_tag="VLI90_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14601.1"
                     /translation="TAGNAVAFSCGVELLAPFSVDITSPSLQQERLEQMQRTVAERKA
                     ADRVEHFQRAAHLLKQWDALKASAPSITPGQLLEQLNPADRGAMLETLIRASAQQGAQ
                     PDLWAVAGPSLVRIDLKGSSPQSTLIALPTTAGPLRSVSVMDGRVLVGARNGVFVVDP
                     ARPEQPETYLDPDLQSEYGFTRVTFGPGNTIFACHRDGGIVGWTIGQYDRPVQQRRTA
                     ELSGSPKNLLLIRGQQELLFTVEQQLMRWTNGEPTTVLSTNGPILSLLDAGDELIAVG
                     EDGFCSLIDPGTLQVRSTFRPAQRVSSAGLLPWLTSHRLLLAQPDGAIAGVGVDDQLM
                     TAYTAAYLGFRDVTASAGKVAAMSADRQRIVLWNAWDGRKPAGEIYLTSLTRHRIADI
                     SFAS"
BASE COUNT          640 a         1187 c         1078 g          594 t
ORIGIN      
        1 cgacccgaag atcaagggcg tgaacctcgc cgtgtactcg acggtgccgc tgggcgcggg
       61 cgtcagctcc agcgccgcga tcgaagtggc gacgatgatg aacctcgtcg agcacttcag
      121 catccgcgca gccgtcgatc cgctgcgcat ggccgcgatg tgccaggccg tcgagaaccg
      181 catcgtgggc gcgccctgcg gcatcatgga tcaagtcagc agcggttacg gccaagccgg
      241 tgcgctgctg cggatgacct gccaaccgca caaggtggaa tccccgctgc acctgccgcc
      301 gggcacgcgc gtgattggca tcaacagcaa cgtgaagcac agcgtcggcg gcggcatgta
      361 cggccgcacg cgctgcgctg ccttcatggg tcaccagatc atcctgacaa agatgcgcga
      421 gatgggcgcg gccgcgggca aagcgctggt cggcgatccg ctcaacggct atctcgccaa
      481 cctcgatcaa gatgattaca agcaactgtt ccgcccacac ctgcccgagc agatcggcgg
      541 tcaggatttt ctcgaccgct ttggcccgac gatcgaccag gcaacgaagg tcgatccgac
      601 gcagagctac cacgttcagc aggcaaccga tcaccacgtc ctggaagcgc ggcgcgtgca
      661 gcaattcgta gaatttctcc aacaagcggc gaaatgccaa ggtctgccgc gaaagaacct
      721 gctcgataaa gcgggtcacc tgatgtacgc gtcgcacctc tcgtacacca acgatgcgct
      781 gctcggcgcc gaagaatgcg atctgctggt gcagctggtg cgcgaccggg agcccgcggg
      841 tttatacggc gcgaaaatca caggcggggg cagcggcggc accgtcgcgg tgttggcgga
      901 gaagtcccca agcgtcgatg ccgcggtgaa ccagatcatg tcgatctacg aacagcgcac
      961 cggccgcaag gccgaagcat tcctaggcag cagccccggc gcctggcacg tcggcaccgc
     1021 ggttgtctaa ttaaaattga agactcccca acaccctttt agcggcggcc cgaagggccg
     1081 agcgttcaaa gtggtcactg ctccacatcg gcgctcgccg cctgcggatc cgcatccccg
     1141 cgccacttat agggcttagg ctcaaatcga taaaagctct tcgcccccgc gaaccgcgtg
     1201 ctggccgtcg cgatctcttc cttcaactgc tccaactccg ggttccgctg aatgtgatcg
     1261 aaggacttca ccgtcacgac gctataccaa ctgcgctgcg cccaacccgc cggcccgctg
     1321 acgaccgtgc acggaatgcc ggccttgttc agaaaatcgc aggcgcgctt ggcgatctcc
     1381 ttatccggat aactctgcgc gatgatgtag tgcagcccga cttgccgcgg gcctttgccg
     1441 tcggtcaccg cggcggccga tggggccgac gccggtgctt taactgcggc ggtcggcgcc
     1501 gcgcgaccaa cgttcatcac cgccggattg gctggcggcg tcgtcaccgc ggccagaaac
     1561 ccagcgctcg ttgtctgccc atgcgccacg ccattgccgc tcgcgccaac ggcggggcgg
     1621 tattcgtcat aggtcgtcgt gcgcgtgccg gccaggtatg cgaacgcgac gatcagcacc
     1681 agcacgaacc ccgcggcggc ggcgccgccg tagctcaact tgaaacgcag ttcgccgttg
     1741 ccgttctcga cgccaacggg ggaatccccg ccgatgcgca gcggcggcgc gggtcgctcg
     1801 atatacacgg gcttctcaat aatgcgttcg ataatccgcg gcggcggctc gaccactggc
     1861 gcgggcgtcg ctggcgcttc gctgcgcgac gaaaacagcg catcgagcga actgcgtttg
     1921 ctcttgaacc accacttggg cgtgggaatc ccgccgctgg gactggcctt gggtggccgg
     1981 cggctggtgt gaatcacttc gaacagggcg gtggcgtgct tacctttggc catccatgac
     2041 ctgcctttcc gatcttcgac ccacatcgcg cccgtcacaa tccattgcga cgcgcacgac
     2101 ggcaataaat gaccgcacgc acctcggccg acccccacag ctgatgaggc gatgcgtcat
     2161 tggtctaacc tacgtttcac cccccggtgg gtcaagccgg tggagcgtga aaataaaacg
     2221 gatattccat ttacgtcgcg acatcgcgca gctcctgcgc gatcgaccag acccgtcgtt
     2281 cacatcggcg gcttcttaag gtgacttcag gacgcgaacg agatgtccgc gatgcggtgg
     2341 cgtgtgaggc tggtcagata aatttccccc gcgggcttgc ggccgtccca cgcgttccac
     2401 agcacgatgc gctgccgatc cgccgacatc gccgcgacct tgcccgccga cgccgtgacg
     2461 tcgcggaagc cgaggtacgc ggcggtgtac gcggtcataa gctggtcatc cacaccgacg
     2521 ccggcaatcg cgccgtcggg ctgtgccaac aacaggcggt gtgaggtgag ccagggcagc
     2581 aaaccagccg aactcactcg ctgcgccggc cggaacgtcg atcgaacctg cagcgtgccg
     2641 ggatcgatca gcgagcaaaa tccgtcttcg cccaccgcga ttaattcatc gcctgcatca
     2701 agcagcgaga ggatcggacc attcgtgctt agcacggtcg tcggttcgcc attcgtccac
     2761 cgcatcaatt gctgctcgac cgtgaagagc aattcctgct ggccgcggat cagcagcagg
     2821 ttcttcggcg aaccgctcag ttccgccgtg cgccgctgtt gaacgggccg atcgtattgc
     2881 ccgatcgtcc accccacgat gccgccgtcg cgatggcagg cgaagattgt gttgcccgga
     2941 ccgaacgtca cgcgggtgaa gccgtattcg ctttgcagat ccggatccag atacgtctcc
     3001 ggctgctccg gtcgcgcggg atcgacgacg aacacgccat tgcgcgcgcc gactagcacc
     3061 cggccatcca tcacgctgac gctgcgcagc ggccctgcgg tcgtcggtag cgcgatcaac
     3121 gtcgactgcg gcgacgatcc cttcagatca atccgcacca gcgacggccc cgcgacggcc
     3181 cacagatccg gctgcgcgcc ctgctgcgcc gacgccctga tcagcgtctc cagcatcgcc
     3241 ccgcggtcgg cggggttgag ttgctccagc aattgccccg gcgtgatgga cggagccgac
     3301 gccttgagcg catcccactg cttgagcaga tgggccgcgc gctggaaatg ctccacgcga
     3361 tcggcagcct tgcgctcggc gaccgtccgc tgcatctgct ccagccgctc ttgctgcaag
     3421 gatgggctgg tgatgtcaac gctgaacggc gcgagcagct cgacgccgca ggagaacgcg
     3481 acggcgttcc ccgcggtgc
//
LOCUS       DATIMH010000583         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5052116, whole genome shotgun sequence.
ACCESSION   DATIMH010000583 DATIMH010000000
VERSION     DATIMH010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5052116"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4033
                     /locus_tag="VLI90_10095"
     CDS             <1..4033
                     /locus_tag="VLI90_10095"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HSV14602.1"
                     /translation="GRSQYSVDPYLNGRVDDFRIYRGALPAGQVYTLATGNNAPAVPV
                     APAAFAATAIPGNQINLSWSAVGGATSYSLYRSTSASGPFWPIATVISGTTYADTDLA
                     ADLSSGTPYYYRVDAANAGGDGPTTSASAIALPPNASVPTSLVAKALSASAISLNWTA
                     STNAATYTVRRSATSGGPYTVIASGLTNPTYTDTGRTVGTTYYYVVTAINASGETAFS
                     NESNATPTDLFVHLKFDDGSGSIAADESGNGWNGTLANSPTWTSAGKVNGGLTLANVS
                     VASNSTSAQTVSLPSGIVAGQTSFTIAAWVNLSTSATWMRLFDFGTGTNNYMFLSVNR
                     GTSNQPRFAILAAGNATEEGINSNISVNTGVWTHLAVTYSNNLGTLYVNGFFAGSNAG
                     MAWSPSTLGTTTQNYIGRSQWAADPVLNGTVDDFRIYAHALTATEVKALATAALPAAP
                     SGLTATPVNGAGQVNLSWSATAVATKYHVYRSASSGGPYTEIAQSVSGTFYQDTNLSP
                     DPNNGTTYYYVISAESAGGEGPQSAQASATLLPALPDSPTTLDVNVAKSGTLALSWDA
                     AANAGSYIIARATSAAGPFTTIATVNGGATTSYTDAGLTNGRVYYYTVTSSNIAGVGE
                     ESPGDSAAPTDLYDYQKFDDATGTAAADASGHGYIGTLINGATWTTSSKIGPDALSLT
                     GASSQYLSLPNGIVSGLTNFSVSAWVYLTTSTNWMRVFDFGTGTGNYMFLTVNRGTSF
                     QPHFGILTTGNGTEQSINGNVSINTGIWTHLAVTWVNNLGTLYVNGAFAGSNSSMTWN
                     PSTLGTTTQNYIGRSQFSADPYLNGRIDDFRIYSRALTLAEVSTIAANTLPSAPSGLS
                     ATVADGQATLTWNVASNAGTYNVKRSLSSGGPYSTITGGISGTSFIDTGLTDGTTYYY
                     VVTGSNATGEGGNSAQLSVTPAPLSYAGTSDNDTYVIRLDPANLSMLQIFVDTPSTGS
                     PTYEVRLSQIPSLSFDGTSGGGDQLAIDFANGNPVPGTGGVSFTGGDTLFIAGTTGAD
                     SFSIDGSHVSNNGVAPITYSNTARVEFDLLGGNDTLTQTAVPGAAVTFNGGAGNDTLN
                     VNAGAFAFTGDAGATSANLSINVANAGSSVNFTGTQHLAALNLASGGAAAMTPAASPA
                     SAAIMNVASVIINGTGSSLNLSNNELLTATALATVQTQLDNSQLWTSVGGGALGSMIA
                     SGQTEVRYTLPGDTNLDGAVDVADLGALATNYGLTSGATWAQGDVNYDGAVNVGDLGM
                     IATSYGATPAFTTTAPAAIAQPAAQAALITSGNVTGAASLNSPFAERPLYDEQADSTL
                     DALLNPRKDHRELRAARHGVLER"
BASE COUNT          831 a         1505 c         1270 g          663 t
ORIGIN      
        1 cggccggtcg cagtattcgg tcgatccgta cctcaacggc agggtggatg acttccgtat
       61 ttatcgaggc gcattgccgg cggggcaggt atacacgctg gcgactggca acaacgcgcc
      121 ggcggtgccg gtggcgccgg cggcgttcgc ggccaccgcg attcccggca atcagatcaa
      181 cctcagttgg agcgctgtcg gcggcgctac cagctactcg ctctatcgct caaccagcgc
      241 gagcggaccg ttctggccca tcgcgacggt catcagcggt acgacctatg ccgacacgga
      301 tctggctgcg gatttgtcca gcggcacgcc gtattactat cgcgttgacg ccgccaacgc
      361 cggcggcgac gggccgacga cgtccgccag cgccatcgcg ctgcccccga acgcatcggt
      421 gccgaccagc ctcgtcgcca aggcgctgtc ggccagcgcg atctcgctca actggacggc
      481 gagcacgaac gccgcgacgt acaccgttcg ccgatcggcc acgtccggtg gaccgtacac
      541 ggtcatcgcc agtggtctga ccaacccgac gtacaccgac accggccgca ccgtcggcac
      601 gacgtattac tacgtcgtca ccgccatcaa cgcgtccggc gaaaccgcgt tctccaacga
      661 gtccaacgcc acgcccactg acctgtttgt tcacctgaag ttcgacgacg gcagcggcag
      721 catcgccgcc gacgaaagcg gcaacggttg gaacggcacg ctggccaact cgccgacgtg
      781 gaccagcgcc ggcaaagtga acggcggcct gacacttgcc aacgtcagcg ttgccagcaa
      841 cagcacaagc gcccaaaccg tctcgctgcc cagcggcatc gtcgccgggc aaacgtcctt
      901 cacgatcgcc gcgtgggtca acctgagcac cagcgcgacg tggatgcgcc tcttcgattt
      961 cggcaccggc acgaacaatt acatgtttct gtcggtgaat cgcggcacca gcaatcaacc
     1021 gcgctttgcc atcctcgccg ccggcaatgc caccgaggag gggatcaaca gcaatatctc
     1081 ggtgaacacc ggcgtctgga cgcacctggc cgtcacctat tccaacaatc tcggcacgct
     1141 gtacgtcaac ggtttcttcg ccggttccaa cgccggcatg gcctggagcc caagcacgct
     1201 gggcacgacg acgcagaact acatcggccg atcgcagtgg gccgccgatc ctgtgctcaa
     1261 cggcacggtg gacgacttcc gcatctacgc ccacgcgctg acggccaccg aggtcaaagc
     1321 gctcgctacc gccgcgctgc ccgcggctcc gagcggtctg acggccacgc cggtcaacgg
     1381 cgcgggccaa gtaaacctca gttggtccgc cactgccgtg gcgacgaagt atcacgttta
     1441 ccgcagcgcg agcagcggtg ggccgtacac tgagattgcc caatccgtgt cgggcacgtt
     1501 ttaccaggac acgaacctga gccccgatcc gaacaacggc acgacgtact attacgtcat
     1561 cagcgccgaa agcgccggcg gcgaggggcc gcagtcggcc caggccagcg cgacactttt
     1621 gccggcgctg ccggattcac cgacgacgct ggacgtgaac gttgccaaga gcggcacgct
     1681 cgcgttgtcc tgggacgccg ccgccaacgc gggcagttac atcatcgcgc gggcaacatc
     1741 agctgcgggt ccgttcacga cgatcgccac cgtcaacggt ggcgcgacga ccagctacac
     1801 cgacgccggc ctgaccaacg gccgcgtgta ctattacacc gtcacctcat caaacatcgc
     1861 cggcgtagga gaggaatcgc ccggtgacag cgccgcgccg acggacctgt acgattacca
     1921 gaagttcgac gacgccaccg gcaccgccgc cgccgatgcc agcggtcacg gctacattgg
     1981 cacgctgatc aacggcgcga cgtggaccac cagcagcaag atcggccccg acgccctcag
     2041 cctcaccggc gcgagcagcc aatacctctc gctgcccaac ggcatcgtct cgggactcac
     2101 caacttctcg gtcagtgcct gggtttacct caccaccagc acgaattgga tgcgcgtgtt
     2161 cgacttcggt accggcaccg gcaactacat gttcctgacg gtgaaccgcg gcaccagctt
     2221 tcagccgcac ttcggcatcc tcaccacggg caacggcacc gagcaatcga tcaacggcaa
     2281 cgtctccatt aacaccggca tctggacgca cctggccgtc acctgggtga acaaccttgg
     2341 cacgctctac gtcaacggtg ccttcgccgg gtccaacagc agcatgacgt ggaacccgag
     2401 caccctgggc acgacgacgc agaactacat cggccgatcg cagttttcag ctgacccgta
     2461 cctgaacggc cgcatcgacg acttccgcat ttactcccgc gccctgacct tagctgaggt
     2521 gagcacgatc gccgccaaca cgttgccgtc ggcgcccagc ggtctgtcgg ccaccgtcgc
     2581 cgatgggcag gcgacgctca cgtggaacgt cgcctccaac gccggcacct acaacgtcaa
     2641 gcgcagcctg agcagcggtg ggccatattc gacgatcacc ggcgggatca gcggcacgag
     2701 cttcatcgac acgggactga ccgacggcac gacgtactac tatgtcgtca ccggctccaa
     2761 cgcgaccggt gagggcggca attccgcgca gctcagcgtc acgcccgcgc cactaagcta
     2821 cgccggcacc agcgacaacg acacgtacgt catccgcctg gacccggcca acctgtcgat
     2881 gctgcagatt ttcgtagaca cgccgagcac cggcagcccc acatacgaag tgaggttgtc
     2941 gcagatcccg tcgctgagct tcgacggcac cagcggtggc ggtgaccagc tcgcgatcga
     3001 cttcgccaac ggcaatcccg tccccggtac cggcggtgtc agcttcaccg gcggcgatac
     3061 gctgttcatc gcgggtacaa ccggcgccga cagcttctcg atcgacggct cgcacgtcag
     3121 caacaacggc gtggcgccga ttacgtactc gaacaccgct cgcgtggagt tcgacctgct
     3181 gggcggcaac gacacgctca cgcagactgc cgtacctgga gcagccgtca cgttcaacgg
     3241 tggcgcgggc aacgacacgc tgaacgtcaa cgcaggcgcg ttcgcgttca ccggcgacgc
     3301 cggcgcgacg tccgcgaatc tgtcgatcaa cgtcgccaac gccgggtcga gcgtgaactt
     3361 caccgggacg cagcacctgg ccgcgctgaa cctcgccagc ggcggggccg cggcgatgac
     3421 accggcggct tcgccggcgt cggcggcgat catgaatgtc gccagcgtca tcatcaacgg
     3481 cactggctca tcgctgaatt tgtcgaataa cgagttgctc acagcgaccg cgctggcgac
     3541 ggtgcaaacg caactggaca acagccagct gtggacgtcg gtcggcggtg gggcacttgg
     3601 atcgatgatc gccagcggac agacggaagt gcgctacacg cttcccggcg acacgaacct
     3661 ggacggcgcc gtcgatgtcg ccgacctcgg cgcgttagcg accaactacg gcctcaccag
     3721 cggcgccacc tgggcccagg gcgacgtgaa ttacgatggc gcggtgaacg tcggcgatct
     3781 tggcatgatc gccaccagct atggcgcgac ccccgccttc acaaccaccg cgcccgcggc
     3841 gatcgcccaa cccgcagccc aagccgcgtt gatcaccagc ggcaacgtca cgggcgccgc
     3901 cagtcttaat tcgccgtttg ccgagcggcc gctgtacgat gaacaggccg attcgacgct
     3961 cgacgccttg ctgaatccac gcaaagacca ccgcgaactc cgcgccgctc gtcacggagt
     4021 attggaacga tgatcaggcg ccgcggcccg atgccatggc cttcgtctat gttgaaccag
     4081 acatcggtca gccaggtgcg aagggtgaac tgagcgtgac tgacgtgcag gccattacgg
     4141 tcggatcggt cgcggtctaa ttcagcgcta acaggttttt ccgacggcgc gggtgacctg
     4201 atttcgcaat ttgagcattg tcgatgaaac ggatcccacg ccgggttcgg agcgtactcg
     4261 cgacgaccc
//
LOCUS       DATIMH010000584         7652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4181128, whole genome shotgun sequence.
ACCESSION   DATIMH010000584 DATIMH010000000
VERSION     DATIMH010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4181128"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VLI90_10100"
     CDS             <1..366
                     /locus_tag="VLI90_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HSV14603.1"
                     /translation="EYFSGDAMRDAFIAVRIAGAVLVVPVMEELFWRDFLWRQILAPN
                     DFKLAAVGEWSLSALLIVSVAFATVHGNWWLTAIVWAMMVGGLLAYTKSLGACIIAHA
                     TTNLLLAVYVLVSQDWAFW"
     gene            complement(432..2120)
                     /locus_tag="VLI90_10105"
     CDS             complement(432..2120)
                     /locus_tag="VLI90_10105"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HSV14604.1"
                     /translation="MKRSRCTVLVCSMALSAMPVLPALAQSGEILTPTPPATPRINGA
                     RVYGQRPGRPFQFTIPATGQDPITYTAEGLPAGLSLDSGTGRITGSVEKPGEFTVTLT
                     ATNTSGKDSKPLVIKIGDEIGLTPPMGWNSWNCFAEAVDQPKVQAAADSFAKLGLIKH
                     GWTYINIDDTWQGARGGKYKGLQANEKFPDMKGLCDHIHSLGLKAGIYSTPWVQSYAG
                     FAGGSSDDPDGAWTKFEKSANWKYDGKYFFADADAKQWSDWGFDYLKYDWNPRSTKVT
                     NEQFHTESETMYNALLHSATRDIIYSYSNSMPFDQIEDQSKMFNCWRTTGDIRDNWAS
                     MSKIGFGQDKWADASRPGHWNDPDMLVVGHVGWGPKLHPTNLTPDEQYTHITLWSLCA
                     APLLIGCDLTQVDDFTVSLLTNDEVLAVDQDPLGKQARRVAGDAKTGTEIWARPLSDG
                     TIAVGLFNRGRNEIIPPQRAKKGEAAPKPVWKLMDHQTEQAEEFASEAEAREALKPLA
                     KPSEVTVNWSDVKLSGAQPVRDIWRQKDLDNADGKFTATVPFHGAVMVKIGKPQ"
     gene            2377..2733
                     /locus_tag="VLI90_10110"
     CDS             2377..2733
                     /locus_tag="VLI90_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSV14605.1"
                     /translation="MKALSVEANQHPSGLIIKIAGDAGVENIDTLERELTKLSAQKPP
                     LIVFDLSGLDFIASLGMGALMSFRKGIARNGGTVRIAAAKPAVLDAFKRAALKELFNT
                     CATVDEALTAKAAGSH"
     gene            2892..3131
                     /locus_tag="VLI90_10115"
     CDS             2892..3131
                     /locus_tag="VLI90_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14606.1"
                     /translation="MKPKDWFVVGVRLIGVWWLVQALQELIYLICFELKYTTSSLYTP
                     GTFVVHAVANLLIGLFLVSGAKLLVSFAGEWGNSD"
     gene            complement(3128..4207)
                     /locus_tag="VLI90_10120"
     CDS             complement(3128..4207)
                     /locus_tag="VLI90_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV14607.1"
                     /translation="MTNLPTHRLRYPALYIYLLGGALGSGLNFLVTFILHRALGVNAY
                     LAFFVGTLANELLHHVYYHVVYVNQEIRLRTPLAIQLALYIIVAIAAASLLWVILRLT
                     GLPFAGAVLIALLILAITNTVINRISTFSSAKLAMVEYEEMGETFYDDQTDPKKVNWF
                     RAWFHRSRYRRLTQFVDSCYKPGMAIADLGCGNCWWNVSGYPVVGVDVNENMLRWAKQ
                     HNRLIDYRITPDLSQTGLADKSMDIVVMSETLEHLLNLEETIREVRRILKDDGVFLIT
                     VPYDYFLGPFFILFNLNCLWQGYVRGSVYHQYRCGHVNHFTKRRLRDALSAAGFRLER
                     LFVVNAMSLYAAAHRADGMAHRPQS"
     gene            complement(4207..5892)
                     /locus_tag="VLI90_10125"
     CDS             complement(4207..5892)
                     /locus_tag="VLI90_10125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV14608.1"
                     /translation="MTRTNGRLVVVGIALLAAVFFLSGINWGLPSRSADRFLFGDRPP
                     WTGEQIISLAGGWDNSADRGADIAMHPLAGRDQPIVLNETDAQRAEIVRRFRLYSCQP
                     DEMITFRSLSRMKPSRGDLDPRFYQYGGLWVYVIGALLKIASLFHFVTLRTDLAYYLD
                     HPEQFARFYIVARLYAAAWGVLGVLVVYALAVEWTGKSIAAITAALLYATMPVVVNMA
                     HEAKPHLPGAVLTLAAVWAAMRYVCVGSLRWWIVSGALCGAAFGMVLTGIVAFAIIPV
                     MVLLRPMSWRRRVSVMLAAGAVGVAVFVATNPYLPLNYLLHRSVVRSNVGNYGNFYKP
                     ELSLPALTNAVRLIGQGMSLPLAMVAAISLCVLAIKATKAHDPAVGCGAENRLTAEPW
                     ALGPLFIAPAALVALQFVLLARDKPAEYARFALTLDVMLAIATAKALSMLTPTAARGA
                     AVMLLALSAVAGGKYVAHFRGDHGDQSTRLRAAIELQRQSRPGRTLAVLAEPAPYCLP
                     PVNLFDWRIVLLPRGGDPSQLPSSDWASVWPQDVSSDAPISWANKPMRIDFKP"
     gene            complement(6063..6719)
                     /locus_tag="VLI90_10130"
     CDS             complement(6063..6719)
                     /locus_tag="VLI90_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14609.1"
                     /translation="MALCGLCIALAGVGCATPPTAITTNPSGAFVAVNGVGAGASPVN
                     YTFDFNKQPVYEVKATKTGYFDSDITLRPDSAGISNGVLTMALPRDEAYEQTTTTEAT
                     NRWLKVEVAGKMTQADAWQKIVDSVTGRYSSIEQMDNASGYLRSVAIARAFKHPVNGE
                     YTIRTQFLGAVSSRDPLVYKMEILAQQSTANGNWVPYDRVFKEDAQLLEEMQARLGTK
                     "
     gene            complement(6960..>7652)
                     /locus_tag="VLI90_10135"
     CDS             complement(6960..>7652)
                     /locus_tag="VLI90_10135"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HSV14610.1"
                     /translation="TALRLFNSSGTQLAANNDGKAPGEGSSKFSYLEYVFPTAGTYYV
                     GVSLNGNTHYDPKTGASTVNGTGPTGDYRLYLNNLGTTSPTVLRVDAGGGNFTDANGD
                     FFQADTGFTGGAKSTTTYAVANTTDDALFATNRIGSSFSFSKTVANGNYQLTLNFADP
                     SVTAAGQRKFNVFAEGKKILSNFDIVATAGAGKSAVSKTFNVTVNDGKIDLSFAGVVG
                     NAEVSGISLVKV"
BASE COUNT         1395 a         2349 c         2430 g         1478 t
ORIGIN      
        1 gaatacttct cgggcgacgc gatgcgcgat gcatttatcg ccgtgcgcat cgcgggggcg
       61 gtgctcgtcg tgccggtgat ggaggaattg ttttggcgcg attttctgtg gcggcagatc
      121 ctcgcgccga acgatttcaa gctggccgcc gtgggcgagt ggagtttgtc ggcgctgctg
      181 atcgtctcgg tcgcgttcgc aacggtgcac gggaactggt ggctgacggc gatcgtctgg
      241 gcaatgatgg tcggcgggct gctggcgtac accaagagcc tgggggcgtg catcatcgcg
      301 cacgcgacga cgaacctgct gctggcggtg tacgtgctgg tctcccaaga ctgggcgttt
      361 tggtgacgcc gcttgcggct tagcaaacga aggtgcgtgg tctgcgaagc cgcaagcggc
      421 ggacggtcag atcactgcgg tttcccgatc ttcaccatca ccgcgccgtg gaatggaacg
      481 gtcgccgtga actttccgtc ggcgttgtcc aggtcttttt gacgccagat atctcgcacg
      541 ggttgtgccc cgctcagctt cacgtcggac cagttgaccg tcacttcact cggcttggcc
      601 agcggcttga gcgcttcgcg cgcttcggcc tcgctcgcga attcttccgc ctgctccgtc
      661 tggtgatcca tcagcttcca caccggcttg ggcgcggctt cgcctttctt ggcgcgctga
      721 ggcggaatga tctcattgcg tccgcggttg aagaggccga cggcgatcgt gccgtcggat
      781 aaggggcgcg cccagatttc cgtgccagtc ttggcgtcgc cggcgacgcg cctggcctgc
      841 ttgccgagcg ggtcctggtc caccgcgagg acttcgtcgt tggtcagcaa gctgaccgtg
      901 aagtcgtcca cctgcgtcag gtcgcagccg atcagcagcg gcgctgcgca caggctccac
      961 agcgtgatgt gcgtgtactg ctcgtccggc gtcaggttgg tcgggtgcag cttcggcccc
     1021 cagccgacgt ggccgaccac cagcatgtcg gggtcgttcc agtgaccggg gcgcgaggcg
     1081 tcggcccact tgtcctggcc aaaaccgatc ttgctcatgc tggcccagtt gtcgcggatg
     1141 tcgccggtcg tccgccagca gttgaacatt tttgactgat cttcgatctg gtcgaacggc
     1201 atgctgttgg agtagctgta gatgatgtcg cgcgtcgcgc tgtgcagcag cgcgttgtac
     1261 atcgtttcgc tttcggtgtg gaactgctcg ttggtcacct tcgtgctgcg cgggttccag
     1321 tcgtatttga gatagtcgaa tccccagtcg gaccactgct tggcgtcggc gtcggcgaag
     1381 aaatatttgc cgtcgtactt ccagttcgcg ctcttttcaa atttcgtcca cgcaccgtcg
     1441 ggatcatccg aactgccgcc ggcgaagccc gcgtagctct gcacccacgg cgtggaatag
     1501 atgccggcct tcaggcccag cgagtggatg tgatcgcaga ggcccttcat gtcggggaat
     1561 ttctcgttgg cctgcagacc tttgtatttc ccgccgcggg cgccctgcca ggtgtcgtcg
     1621 atgttgatgt acgtccagcc gtgcttgatc aagccgagct tggcgaagct gtcggcggcc
     1681 gcctgcacct tcggttgatc gaccgcctcg gcgaaacagt tccagctgtt ccaacccatc
     1741 ggcggcgtga gaccaatttc gtcaccgatc ttaatgacca gtggcttgct gtctttgccc
     1801 gacgtgttgg tggcggtgag cgtgaccgtg aattcacccg gcttctcgac cgaacccgta
     1861 atccgaccgg tgcccgaatc gagcgacagt cccgcgggca atccctcggc ggtataggtg
     1921 atcgggtcct gccccgtcgc cgggatggtg aactggaacg gccggccggg gcgttggccg
     1981 tacacgcgcg ccccgttaat ccgcggcgtc gccggcggcg tcggggtcag gatttcgcca
     2041 ctctgcgcga gcgccggcaa caccggcatc gccgacagcg ccattgagca aaccagcacg
     2101 gtacatcgcg aacgcttcat aggaggatct ccacagctac ccgtgtcatg ggctgctaat
     2161 gtcatgtgta ccgcgtccat cgccgcgccg ttgtgtagaa gaatggtcga tccgcacata
     2221 ccgcttgcga cggctcgcct gggcggcgat ggcggaacga gcgcgagagc tgtctgtcaa
     2281 atacttttga ccaaacccgc cccaccgcgt tatgctgccg gcgcaaattg caccgctccg
     2341 ccattcgtcc catcgtgcgc ctgcgggagg accggcttga aagccttaag cgttgaagcg
     2401 aatcagcacc cgtccggcct gatcatcaag atcgccggtg acgccggtgt tgagaatatc
     2461 gatacgctcg agcgcgagct gaccaagctc tcggcgcaaa aaccaccgct gatcgtcttc
     2521 gacctgtccg gcctggactt catcgccagc ctgggcatgg gggcgctgat gagctttcgc
     2581 aaaggcatcg cccgcaacgg cggcaccgtg cgcatcgccg ccgccaaacc cgccgtgctg
     2641 gacgcgttca agcgggcggc gctcaaagag ctgttcaaca cgtgcgcgac cgtcgacgaa
     2701 gcgctgaccg ccaaggccgc cggatcgcat tgaagctttg aacttctaaa aaatatcggg
     2761 atatgctgta gctgcgacgc ctgcttcgcc ttgtttttcc gagccgaaca ggcgacgaag
     2821 gcgtcgcagc taccaatatt tctagaagct tttggtctac cgcggccctg gaagaaatgg
     2881 ggagagtgac aatgaagcct aaagattggt tcgtcgtggg tgttcgctta atcggggttt
     2941 ggtggttggt tcaggcgctg caggaattga tctacctcat atgctttgaa ctgaagtaca
     3001 cgacgtcgtc gctttacacg ccggggactt tcgtcgtaca cgctgtcgca aacctgctca
     3061 tcgggctttt tctcgtctcg ggagcgaagc tgctggtgtc gttcgctggc gagtggggca
     3121 atagcgacta agactggggc ctgtgggcca tcccatctgc gcgatgggca gccgcataca
     3181 gcgacatcgc gttgacaacg aacaaccgct ccagccgaaa tcccgccgcc gacagtgcgt
     3241 cgcgcaaccg ccgttttgta aaatgattca cgtgcccgca gcggtactgg tggtacacgc
     3301 tcccgcggac atagccctgc cacaggcaat tcaagttgaa caggatgaag aacgggccga
     3361 ggaaatagtc gtacggcacc gtgatcagga acacgccgtc gtctttcaag atgcgccgga
     3421 cttcgcgaat cgtctcttcc aggttcagca ggtgctcgag cgtttcggac atgacgacga
     3481 tgtccatcga tttgtccgcc agccccgtct gcgacaggtc tggcgtaatg cggtagtcga
     3541 tcagtcgatt gtgctgcttc gcccagcgaa gcatgttctc gttcacgtcg acgccgacga
     3601 cggggtagcc gctcacgttc caccagcagt tcccgcagcc taagtcggcg atcgccatgc
     3661 cgggcttgta gcagctgtcc acgaactgcg tgagccggcg atagcgcgag cggtggaacc
     3721 acgcgcgaaa ccagttcact ttcttcgggt cggtctggtc gtcgtagaag gtctcgccca
     3781 tctcttcgta ttcgaccatc gccagcttgg cggagctgaa cgtcgaaatg cggttgatga
     3841 cggtgttggt gatcgcgagg atcaacagcg cgatgagcac ggcgcccgcg aagggcaagc
     3901 cggtcagtcg caggatcacc caaagcagcg acgccgcggc gatggcgacg atgatgtaca
     3961 gcgccagctg aatcgccagc ggcgtgcgca ggcgaatctc ctggttcacg tacacgacgt
     4021 ggtagtaaac gtgatgcagc aattcgttgg cgagcgtgcc gacgaagaac gcgagatatg
     4081 catttacgcc cagcgcgcgg tggaggatga acgtgacgag gaagttcagc ccggaaccca
     4141 gcgcgccgcc gagcaggtag atgtacagag cgggatagcg cagccggtgc gtgggcaggt
     4201 tcgtcatcag ggcttgaagt ctatacgcat cggcttattt gcccagctaa tgggcgcatc
     4261 cgacgacacg tcctggggcc aaacgctcgc ccagtcgctt gatggaagtt gcgacggatc
     4321 gccgccgcgc ggcagcagga cgatccgcca gtcgaacagg ttcaccggtg gcaggcaata
     4381 cggcgccggc tcggcgagca cggcgagcgt gcggccgggg cgactctggc gctgcagttc
     4441 aatcgcggcg cggagccgcg tcgattgatc gccatggtcg cctctgaagt gtgcaacgta
     4501 ctttcccccg gcgaccgccg agagcgcgag cagcatgacc gccgcgcctc gcgcggcggt
     4561 gggtgttagc atcgagagcg ctttggcggt tgcgatcgcg agcatgacgt cgagcgtcag
     4621 ggcgaagcgc gcatactccg cgggcttgtc gcgcgccagt agtacaaatt gcaacgctac
     4681 cagagctgcg ggggcgatga aaagcggtcc caaagcccac ggctctgccg tgagacgatt
     4741 ctcggcgccg catcccacgg cagggtcgtg ggctttggtt gctttgattg caagaacaca
     4801 aagcgaaatc gccgccacca ttgccagtgg aagtgacatg ccttggccaa tcaatcgcac
     4861 cgcgttcgtc agcgccggca gcgacaactc cggcttgtag aagttcccgt agttccccac
     4921 gttcgaacgc acgacggatc ggtgcagcag gtaattcaac ggcaagtatg gattggtagc
     4981 aacgaacacc gccacgccca cggcgccggc ggcgagcatc accgagactc gccgtcgcca
     5041 cgacattggc cgtagcagga ccatcaccgg aatgatcgcg aaggcgacga tgcccgtcag
     5101 caccatgccg aacgcggcgc cgcacagcgc tccgctaacg atccaccatc gtaatgagcc
     5161 gacgcagaca taccgcatcg ccgcccatac cgcggcgagc gttagcacgg cgcccggcag
     5221 gtgcggcttg gcttcgtggg ccatgttcac cacgaccggc atcgtcgcgt acagcaacgc
     5281 cgcggtgatc gcggcgatcg acttgccggt ccactccacc gccagcgcat acacgaccag
     5341 cacgccaagc acgccccacg ccgccgcgta aagtcttgcg acgatgtaga agcgcgcgaa
     5401 ttgttccggg tgatcgaggt agtacgccag gtcggtccgc agcgtgacga aatggaacag
     5461 cgaggcgatc ttcagcagcg cgccgatcac gtacacccac agtccgccgt actggtagaa
     5521 ccgcgggtcg agatcgccgc gcgatggctt catccgcgac agcgagcgaa acgtgatcat
     5581 ctcatccggc tggcaggagt agagccggaa gcggcggacg atctcggcgc gctgcgcatc
     5641 ggtttcgttt aagacaatgg gttgatcgcg accggcgagc gggtgcatcg cgatgtccgc
     5701 cccgcgatcg gccgagttgt cccacccgcc agcgagggag atgatttgct cacccgtcca
     5761 gggcgggcga tcgccgaaga gaaatcgatc tgcgctgcgt gacggcaagc cccagttgat
     5821 gcccgaaaga aaaaagacgg cggcgagaag cgcgatcccc accaccacca gccgcccgtt
     5881 cgtgcgagtc atgtgcctga agtgtagcgt cgcggcggcg ccgcgctcaa atcagctcgc
     5941 cttcaccaaa accaatcgca gccgcgccgc ttcagcccgc ggaccgctgg acgtcgaacg
     6001 tggttcgatc ttccaacggt ccgcgggcta aagccacgcg gctgcgacgt gtgccgtttg
     6061 agttacttgg ttccgaggcg ggcctgcatc tcttcgagca gctgcgcgtc ttccttgaac
     6121 acgcgatcgt atggcaccca attgccgttg gcggtggact gctgggcgag gatctccatc
     6181 ttgtaaacca gcggatcgcg cgacgaaacc gcgccaagga attgcgtgcg gatggtgtat
     6241 tcaccgttca ccggatgctt gaacgcgcgc gcgatggcaa cgctgcgcag atagcccgaa
     6301 gcgttgtcca tctgctcaat gctggaatag cgcccggtga ccgaatcgac gatcttctgc
     6361 cacgcatcgg cttgcgtcat cttccccgcc acctcgacct tcagccagcg gttggtcgcc
     6421 tcggtggtgg tggtctgttc atacgcctca tcgcgcggca gcgccatggt gagcacgccg
     6481 ttgctgatgc cggcgctatc gggccggagc gtgatgtccg aatcgaagta accggtcttg
     6541 gtcgccttca cttcgtagac cggctgcttg ttgaagtcga aggtgtagtt cactggcgaa
     6601 gcccccgcgc caacaccgtt gacggcgacg aacgcgccgg atggatttgt ggtgatcgcc
     6661 gtgggcggcg tcgcgcagcc gacccccgcc aaggcaatac acaagccgca caatgccact
     6721 gaaatccgcc ggatcataag cgctccattc atcgccagag gtgacgcgca acaaaagtgt
     6781 gcaacggtag gtgtgtcggc gtttggagcg cgttgccttg aaggcaacag ccatgcgggc
     6841 gcgaagcgtg gcaccgttcg atgaaaacgg aacgcccgat aaatgcgcat agcggtcgcc
     6901 cggcaagggg cgaccgctat gcagaggcat tgcccacccg agacgcatga gcatctcgat
     6961 cacaccttca ccagcgagat gccggagacc tcggcgttgc cgacgacgcc ggcgaagctc
     7021 aggtcgatct tgccgtcgtt caccgtgacg ttgaacgtct tgctgacggc gctcttgccc
     7081 gcgccggcgg tggcgacgat gtcgaagttg ctcaggatct tcttgccttc ggcgaagacg
     7141 ttgaacttcc gctgcccggc cgccgtcacc gacgggtcgg caaagttcag cgtcaactga
     7201 tagttcccat tggcgaccgt cttggagaag ctgaagctgc tgccgatgcg gttggtcgcg
     7261 aacaacgcat catccgtcgt gttggcgacg gcataagtcg tcgtcgactt cgcgccgccg
     7321 gtgaaaccgg tgtcggcctg gaagaagtcg ccgttggcat cggtgaagtt gccgccaccg
     7381 gcgtccacgc gcaggacggt cggcgacgtc gtgccaaggt tgttcaggta caggcgataa
     7441 tcgccggtcg ggccagtgcc gttgacggtg ctcgcgccgg tcttgggatc gtagtgcgtg
     7501 ttgccgttca gcgacacacc gacgtaatac gtgccggcgg tcgggaagac gtactcgaga
     7561 taagagaact tgctcgaacc ttcgcccggc gctttgccgt cgttgttggc agccagctgc
     7621 gtgccgctgg agttgaacag ccgcaacgcc gt
//
LOCUS       DATIMH010000585         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3606148, whole genome shotgun sequence.
ACCESSION   DATIMH010000585 DATIMH010000000
VERSION     DATIMH010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3606148"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VLI90_10140"
     CDS             complement(<1..643)
                     /locus_tag="VLI90_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HSV14611.1"
                     /translation="MKHATVRQLRRAALAALFVLLFVLPTIAGAANVPATGPAGPPAV
                     PHGTIAQRAQSLIGLTAFTALAWIIGRAMGARAHIRLRTLAWGLLLQFIFGAVVVWNR
                     AFLVLINALVDALLGFTAEGAKLVFGDLAAQGGAAVADANNQVIGYAHSVGYFAFFVL
                     PTIIFFACLTAVAYHSGIMQYVVQGLAWIMAKSMGCSGGETLSSAANIFVGQTE"
     gene            complement(936..2579)
                     /locus_tag="VLI90_10145"
     CDS             complement(936..2579)
                     /locus_tag="VLI90_10145"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV14612.1"
                     /translation="MTTDDLFQHAVRHAQAGRLADAIDLLRQVLAADPSHIAALNNLG
                     VCLRQSGRGAEATNHFRQALAIAPDRPHVWNNLGNALRGAGRLDEAASAYERCLSLDS
                     NNTEALSNLGTCRKDGGRIDEALALYRRSLATSPSPVVHGNLLYAMHFQEAVDSLELL
                     REHRAWAARYADPLRERWQPIARDPTRRRLRIGYVSPDLRLHPVGRFLLPLLRHHDRR
                     HFDIFCYATNRAPDDAFSQRLRDHADHWRSAGAMSDDELAALIRADGIDILVDLSLHM
                     AGNRLLAFARRPAPVQVTYLGYCGTSGMGAMDFRLTDPHLDPPGGDDARYSEDSVRLP
                     ETYWCYEPATDDVPVQPPPSAAMGRITFGCLNNFAKVSDVALGAWAEVLRAVPRSSLL
                     LHAHQGSHRHRVSEAMTHRGIDPARVDFVGFVPLDEFFRLHDRIDIALDPFPYAGGTT
                     TCDALWMGVPAVTLAGPTAVGRGGASILTNVGLAELIAGSMEQYMHIAAELAEDRARL
                     AELRRSLRDRMQQSPLMDGDRFARHIEAAYRQMWVAGQT"
     gene            complement(2576..>3136)
                     /gene="ruvX"
                     /locus_tag="VLI90_10150"
     CDS             complement(2576..>3136)
                     /gene="ruvX"
                     /locus_tag="VLI90_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HSV14613.1"
                     /translation="RRRCLSVLAKTRVFGVPQTPASGFHRFPSRLLKEMRTLAIDLGA
                     RRVGLALSDAGGRWATPHDVLNVSDPAQAVAPILKLVHEEDVQQLVVGLPLNMDGTTG
                     PAARKTIEWATGLAASAARSLGPLKIIFVDERLSSFEAEQQLTDRKRAGEKLTRKRKK
                     EQLDAIAAAGFLQAFLDGKLAPIDLP"
BASE COUNT          581 a         1054 c          997 g          504 t
ORIGIN      
        1 cttcggtctg accgacgaaa atattcgcgg ccgacgaaag cgtctccccg ccgctgcagc
       61 ccatgctctt ggccataatc cacgccaagc cctgcacgac atactgcatg atgccgctgt
      121 gatacgcgac ggccgtcagg caggcgaaga agatgatcgt cggcagcacg aagaacgcga
      181 aatatccaac ggaatgggcg taaccgatca cttgattgtt cgcatccgcc acggccgccc
      241 cgccctgggc ggcgagatcg ccgaacacca gcttcgcgcc ttcggcggtg aagccaagca
      301 gcgcatcgac cagcgcgttg atcagcacga gaaaggcgcg gttccacacg accaccgcgc
      361 cgaagataaa ctgcaggagt agtccccacg ccagcgtgcg caggcggatg tgcgcgcggg
      421 cgcccattgc ccgaccgatg atccaggcca gcgccgtgaa tgccgtcaaa ccaatcagtg
      481 actgcgcccg ctgcgcgatg gtgccatgcg gaacggccgg tggacccgct ggccctgtcg
      541 cgggcacgtt ggcagcgccg gcgatcgtcg gcagcacaaa gagcaaaacg aacagcgcag
      601 caagagcggc gcggcgcagc tggcggactg tggcgtgttt catatggcga tcatcgacag
      661 cggacgatga ccgcgccgct gcgattgctt tgatgttggt atccccgcaa aaagatacgg
      721 caacggcgga cagtcgcaag ttcaccggcc gctgccgaca tccgggcaac ggtgtccggc
      781 tccttccccc tccccccggt actgctgacc gcaggtcgcc gggagagggt cggggtgagg
      841 gcgatgtgga atttcgaacg gctcccgaaa acgaaatcac cctcacccta gccctctccc
      901 ggcgtaccgg gagaggggac cagagcgccc accgcctacg tttgccccgc aacccacatc
      961 tggcgatacg ctgcctcgat gtgccgtgca aagcggtcgc cgtccattaa cggcgattgt
     1021 tgcattcgat cgcgaagcga acgtcgcaac tcggcaagtc gtgcacgatc ttcggccagc
     1081 tccgctgcga tatgcatgta ctgctccatc gaccccgcga tcagttccgc cagcccaacg
     1141 ttcgtcaata tactcgcgcc cccgcgacct acagccgttg gcccagcgag cgtcaccgcc
     1201 ggcacgccca tccaaagcgc atcgcacgtc gtcgtcccgc cggcgtatgg gaacggatcg
     1261 agcgcgatgt cgatccggtc gtgcaacctg aagaactcgt cgagcggaac gaagccgacg
     1321 aagtcgacgc gggccggatc gatgccgcga tgagtcatcg cctcagacac gcgatggcga
     1381 tggctgcctt ggtgcgcatg cagcagcagc gacgaccgcg gcactgcgcg tagcacctcc
     1441 gcccaggcgc ccagcgcaac gtcgcttact ttcgcgaagt tgtttagaca accgaaggtg
     1501 attcgcccca tcgccgcact cgggggcggt tgaaccggca cgtcgtccgt cgccggctcg
     1561 tagcaccagt aggtctccgg cagacgcacc gagtcctccg aataccgcgc atcatccccg
     1621 cccggcggat cgagatgcgg atcggtcaac cgaaaatcca tcgcccccat cccgctcgtc
     1681 ccgcagtaac ccagataagt cacctgcacc ggcgcgggcc ggcgggcgaa agcgagcagg
     1741 cgattgcccg ccatgtgcag gctgagatcg acgaggatgt cgatgccgtc ggcgcgaatc
     1801 agcgcggcga gttcgtcatc gctcatcgcg ccggcggatc gccaatggtc cgcgtgatcg
     1861 cgcagccgct gtgagaacgc atcgtccggt gctcggttgg tggcgtagca gaagatgtcg
     1921 aagtgacggc ggtcgtgatg ccgcagcagc ggcagcagga accggccaac cggatgcagc
     1981 cgaagatcgg gcgagacgta gccgatgcgc aatcgccgac gcgtcgggtc gcgcgcgatc
     2041 ggctgccacc gctcgcggag cggatcggcg tagcgcgcgg cccacgcgcg gtgctcgcgc
     2101 aacagttcga gcgaatcgac cgcctcctga aaatgcatcg cgtaaagcag attgccgtgc
     2161 acgaccggcg acggcgatgt ggccagcgat cggcggtaca gcgcgagcgc ttcgtcgatc
     2221 cggccgccgt ctttgcggca cgtgcctagg ttcgacagcg cttcggtgtt gttcgaatcg
     2281 agcgacaggc accgctcata cgcgctcgcc gcctcgtcca gtcggccggc gccgcgcagg
     2341 gcgtttccta gattattcca cacgtgcgga cggtctggcg cgattgccag cgcttggcga
     2401 aaatgattgg tcgcctcagc gccgcggccg gattgacgca ggcagacgcc gaggttgttc
     2461 agcgctgcga tatgcgacgg gtcggccgcg agcacctggc gcagcaggtc gatcgcgtcg
     2521 gcgagccggc ccgcctgcgc gtgccgcacc gcgtgttgga acagatcgtc ggttgtcacg
     2581 gcaggtcgat cggcgcgagc ttgccgtcca ggaacgcctg cagaaacccc gcggcggcga
     2641 tcgcgtcaag ctgctctttt tttcgcttgc gcgtcagctt ttccccggcg cgcttgcggt
     2701 cggtcaactg ctgctcggct tcaaaactgc tgaggcgttc atccacgaag atgatcttca
     2761 acggcccgag actcctagcc gctgaagcgg ccaggccggt cgcccattcg atcgtcttgc
     2821 gcgccgcggg gccggtggtg ccgtccatgt tcaacggcag acccacgacc agctgctgga
     2881 cgtcttcctc gtgtaccagt ttcaaaatgg gagccaccgc ctgcgcgggg tcggacacat
     2941 tcagcacatc atgcggcgtc gcccagcgtc ccccggcgtc gctgagcgca aggcccacgc
     3001 gacgggcgcc aagatcgatc gcgagcgttc gcatttcttt caggaggcgc gacggaaatc
     3061 gatgaaaccc cgacgccggt gtctgaggta ctccgaaaac ccgggtcttc gcgagtacgc
     3121 tcagacaccg gcgtcg
//
LOCUS       DATIMH010000586         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2160667, whole genome shotgun sequence.
ACCESSION   DATIMH010000586 DATIMH010000000
VERSION     DATIMH010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160667"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VLI90_10155"
     CDS             <1..594
                     /locus_tag="VLI90_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HSV14614.1"
                     /translation="RAAGGAAVMISLNAVGLSGVKKVCDQGAMVIHGHRNGWGMLNRH
                     PMLGIEFPAYQKLWRLAGVDQIHVNGIANKFWESDDSVVRSIEACLKPMLGGFSTLPV
                     VSSGQTGLQAPETWRRTQTRDLLYMAGGGIMAHPAGPAAGVAAIHQAWEAAMSGQDLN
                     RYATTHAELRQSLEKFGGGQSSTGSEPLALSPVSGTT"
     gene            627..>1936
                     /locus_tag="VLI90_10160"
     CDS             627..>1936
                     /locus_tag="VLI90_10160"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HSV14615.1"
                     /translation="MNNLLLSFVGDDFTGSTDAMESLARSGVRTALFTEPPTDEQLAK
                     YPGLRAFGIAALTRSLPAEEMEQALRPIFQSLRQTGAPIVHYKVCSTFDSSPAVGSIG
                     RVIDVGMEAFGSRFVPVVVGAPDLGRYCVFGNLFARCGSESEPYRLDRHPSMSRHPVT
                     PMDEADLRRHLARQTSKSIGLLDLLQLESPAATVRERFDAMVASGQEVMLIDTLYPRH
                     LMKIGQLLAEKASERPPLFVVGSSGVESALCAHWKDAGVIAGEAAFAAPAYVGPIVVV
                     CGSCSPVTAQQIDWAAGNGFAMVELSVHDLLSENVERAMRDAIASAASAVNGGHSVVI
                     RTGASRTAGVEGADRHRIGISLGKLLRRLLENVAVRRLLIAGGDTSSQVARALGIAAV
                     EMIGELTRGSPLCCATAPDSPADGVQITFKGGQIGAVDFFGSVEA"
BASE COUNT          323 a          569 c          690 g          354 t
ORIGIN      
        1 cgcgccgccg gcggcgccgc cgtcatgatc agcctgaacg cggtcgggtt gtccggcgtg
       61 aagaaggtct gcgaccaagg cgcgatggtc atccacggcc accgcaacgg ctggggaatg
      121 ctcaaccgcc atccgatgct cggcatcgaa ttcccagcgt atcaaaaact ctggcgactg
      181 gccggcgtgg accagatcca cgtgaacggc atcgccaaca aattctggga gagcgatgat
      241 tcggtcgtgc gatcgatcga agcgtgcctc aagccgatgc tcggcggctt ctcgaccttg
      301 ccggtcgtct catccggcca aaccggactg caagcgcccg agacgtggcg acgcacgcag
      361 acgcgcgatc tgctgtacat ggccggcggc ggcatcatgg cgcaccccgc cggtcccgcg
      421 gccggcgtgg cggcaatcca ccaggcgtgg gaagcggcga tgagcgggca ggatttaaat
      481 cgatacgcga cgacgcacgc ggagctgcgg caatcgcttg agaaatttgg cggcggacaa
      541 agtagcacgg gctccgagcc tttggctctc tcgcccgtgt ctggaaccac ctgacacggg
      601 ccctggcgtc cgtgctacgg cacatggtga acaatctgct tctcagcttc gttggcgacg
      661 atttcaccgg gtctacggac gcgatggagt cgctggcacg ctccggggtg cgcacggcac
      721 tgttcaccga gccgccgacg gatgagcagc tggcgaaata tcctggcctg cgcgcgttcg
      781 gcatcgcggc gttgacgcgg tcgttgccgg cagaggagat ggagcaggcg cttcgcccga
      841 tcttccaatc gctgcggcaa acgggcgcgc cgatcgtgca ttacaaagtc tgctcgacgt
      901 tcgattcatc gccagccgtc ggcagcatcg ggcgcgtgat tgatgtcggc atggaggcat
      961 tcggttcgcg gtttgtaccg gtcgtcgtcg gcgcgccgga ccttggccgc tactgtgtgt
     1021 ttgggaatct cttcgcgcga tgcggcagcg aatcggagcc gtatcggctg gaccgccatc
     1081 cgtcgatgag ccggcacccg gtgacgccga tggacgaggc cgacctgcgg cggcatctgg
     1141 cgaggcagac gagcaagtcg atcggactgc tcgacctgct gcagctggaa tcaccggcgg
     1201 cgacggtacg cgaacgattc gatgcgatgg tggcgagcgg tcaggaagtc atgctgatcg
     1261 acacgctgta tccgcggcac ttgatgaaga ttggccagct gctggcggag aaggcgagcg
     1321 aacggccacc gctgttcgtc gtcggatcgt cgggggtcga gtcggcgttg tgtgcgcatt
     1381 ggaaagatgc cggtgtcatt gccggcgaag cggcgttcgc cgcgccggcg tatgtcgggc
     1441 cgatcgtcgt cgtctgcggg agttgttcgc cggtcaccgc tcagcagatc gactgggcgg
     1501 cggggaacgg ttttgcgatg gtggaactga gcgttcacga tctgctttcg gaaaatgttg
     1561 aacgcgcgat gcgcgatgcg atcgcaagtg ccgccagcgc cgtcaatggc gggcatagtg
     1621 tcgtcattcg caccggagcc agtcgcacgg cgggtgtcga aggcgcggat cgtcatagga
     1681 tcgggatctc gctcggcaag ctgctgcggc ggttgctgga gaacgtcgct gtccgtcggc
     1741 tgctgatcgc gggtggggac acgtccagcc aggtggcgcg ggcgctgggc atcgccgcgg
     1801 ttgaaatgat tggggagctg acgcgcggct cgccgctgtg ctgcgcgact gcgccggatt
     1861 cccctgcgga tggggtccag atcacgttca aaggtggtca gatcggcgcg gtcgattttt
     1921 ttgggagcgt ggaggc
//
LOCUS       DATIMH010000587         1876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_720955, whole genome shotgun sequence.
ACCESSION   DATIMH010000587 DATIMH010000000
VERSION     DATIMH010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720955"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VLI90_10165"
     CDS             <1..375
                     /locus_tag="VLI90_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14616.1"
                     /translation="TVFSGQLQSPDVQCHLASGVDVRVPIALIQNYSNPQSKAPTMML
                     DRIKAIVADLNADDWKQRDNAEHQLVNLGPAVAGTLKSLREKQPPEAQQRIDSVLKQL
                     ENQPKERAPEPAPISGGGVVDQ"
     gene            complement(433..>1876)
                     /locus_tag="VLI90_10170"
     CDS             complement(433..>1876)
                     /locus_tag="VLI90_10170"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HSV14617.1"
                     /translation="AKIAYDDSAKAILGSADKMEAAALGRQRSAHLANPADQFKSLQA
                     ELMRAQTRSGEVARQFMPNHPTVKSAQGKVDQLIVASAVAAEQWWEQCQAKEDALQNS
                     LDDAQHAALDQQAHAVEFMQLQSDVNQLKKMDEVADSRVKDLDLIRNAGATNIMVLNE
                     AAIAGPPKPLKGRTLLASLVVGIVAGLGAGFVREHLDDRLPNAEAIKSSAGAPVLGSI
                     PTITSGFNAQDRGQVVHHDPFNDAAESYRALRAALQSGLSPRSKTLLITSPTAGDGKS
                     TFISNLGIAMAQASKRVLIVDADLRAPVQHRLFGLKDRIGLCTVITTGDPPAQAVQRT
                     EIEGLDLLPCGPVPVSPLDILNDPALTDHLNDLADQYDVVLIDSPAAATAVDARVLAA
                     SADVTLLIVRSDTATRRQVEETRDGLRGVGARVIGVAINAVTRGGMRARPVTAALPAR
                     SSLATSNNLRAAARRAERKIDPASRPKTRA"
BASE COUNT          304 a          608 c          634 g          330 t
ORIGIN      
        1 accgtcttca gcggtcaact gcaatcgcca gacgtgcaat gccacctcgc cagtggcgtc
       61 gatgtgcgcg tgccgatcgc gctgatccag aactattcca atccacagtc gaaggcccca
      121 acgatgatgc tcgaccgcat caaggcgatc gtggcggact tgaacgcgga tgattggaag
      181 cagcgcgaca acgccgagca ccagctggtg aacctcggcc ccgcggtggc ggggacgctg
      241 aagagcctgc gcgagaaaca accgcccgaa gcgcagcagc ggattgattc ggtgttgaag
      301 cagttggaga accagccgaa ggagagggca ccggaaccgg cgccaatttc cggtggggga
      361 gtggtagatc agtgagacgg cgcgcgcacc gcacgaaaat ccccggatag ctatccgtgg
      421 gctttagcct gctcaagcgc gcgtcttcgg acgtgacgcg ggatcgatct tgcgttccgc
      481 gcgccttgct gcggcgcgca agttgttcga tgtcgcaagg gatgaacgcg ccggcaatgc
      541 cgcggttacc gggcgcgccc gcatcccgcc gcgggtgacg gcgttgatgg cgacgccgat
      601 cacgcgcgcg cccaccccgc gaagtccgtc gcgggtttct tcgacttgcc ggcgagtggc
      661 ggtgtcggag cgcacgatca gcagcgtgac gtccgccgac gccgcgagca cgcgggcgtc
      721 caccgccgtc gcggcggcgg ggctgtcgat caggacgacg tcgtattggt cggccaggtc
      781 gttgagatga tccgtcaggg cgggatcgtt caagatatcg agggggctga ccggcaccgg
      841 cccgcagggc agcagatcca agccttcgat ttccgtgcgc tgaaccgcct gcgccggcgg
      901 atcgccggtg gtgatcacgg tgcacaaccc gatgcggtcc ttcaggccga acaggcggtg
      961 ttgcaccggg gcgcgcaggt cggcgtccac gatcaacacg cgcttcgacg cctgcgccat
     1021 cgcgatgccc aggttgctga tgaacgtgga tttgccgtcg ccagccgtgg gggaggtgat
     1081 cagcagcgtc ttgctgcgcg gcgagagtcc cgactgcagc gccgcccgca gcgcgcgata
     1141 cgattcggcc gcgtcgttga acggatcgtg atgcaccacc tgcccgcggt cttgcgcgtt
     1201 gaagccgctg gtgatcgtcg ggatggaacc gagcaccggc gccccggcgg atgacttgat
     1261 cgcttcagcg tttggcaggc gatcgtccag gtgctcgcgc acgaagccgg cgccgagccc
     1321 ggcaacgatg cctacaacaa gcgacgccag cagcgtccgc cccttcagcg gtttgggcgg
     1381 cccggcgatc gcggcctcgt tcaggaccat gatgttcgtc gcgccggcgt tgcggatcag
     1441 atccaaatct ttcacgcgcg aatccgccac ctcgtccatc ttcttcagct ggttcacgtc
     1501 cgactgcagc tgcatgaact cgaccgcgtg ggcctgctgg tccaacgccg cgtgctgggc
     1561 gtcgtccagg gagttctgca gcgcatcttc cttcgcctgg cactgttccc accattgctc
     1621 ggccgcgacg gcgcttgcga cgatcagctg atcgaccttg ccctgcgccg acttcaccgt
     1681 cgggtggttc ggcatgaatt gccgggcgac ttcgccggag cgcgtctggg cccgcatcag
     1741 ttccgcttgc agggatttga attgatcggc cgggttagcc aggtgggccg accgttggcg
     1801 ccccagcgcg gcggcttcca tcttgtcggc gctgccgagg atcgccttgg ccgaatcgtc
     1861 atatgcgatc ttcgcg
//
LOCUS       DATIMH010000588         5407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2161282, whole genome shotgun sequence.
ACCESSION   DATIMH010000588 DATIMH010000000
VERSION     DATIMH010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161282"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VLI90_10175"
     CDS             <1..358
                     /locus_tag="VLI90_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14618.1"
                     /translation="LWEISAMSDDPLPPSHIDYRPQPEPPPGWPPPRTQRIQGLLAGS
                     LLSLIVWPVAWKFGQQGATLAYIIIGMIALKVIASTVCIVSPRWRPMGAGILASMGVG
                     FLIFFGTCSLTALRGI"
     gene            385..1815
                     /locus_tag="VLI90_10180"
     CDS             385..1815
                     /locus_tag="VLI90_10180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSV14619.1"
                     /translation="MSDRPYLFYELTNSLCSKCLRKVETKVVIQDDRVYLHKWCPEHG
                     REKVLISTDVEYYKLSRQTLKPGQMPLKFQTPIKYGCPFDCGLCPDHEQHSCLTLLEI
                     TDQCNLTCPVCYSDSSPRRTTHRSLAQIEFMLDCIVRNEAEPDVVQISGGEPTIHPQF
                     FDVLDLAKSRPIKHLMLNTNGIRIANDPDFARRLKDYMPGFEIYLQFDSMNPKVLREL
                     RGEDLSDIRRRALDRLNEHNISTTLVVTLKKGLNDGEIGETIDFALKQKCIRGITFQP
                     IQHAGRLEHFDPATDRLTLGEVRQQILAQSPIFKPSDVIPVPCHPDCLAMAYALKLDG
                     CVTPLTGMIDPQILLHGGGNTIVYEKHDSIRGELFRLFSTAASPGSSAASLKQLLCCL
                     PMVEVEGTAITYNNIFRIIIMQFLDAHNFDVRSVKKSCVHIVHPDGRIIPFDTFNLFY
                     RDDRERMLEPMRREQQLVTLGRGMSS"
     gene            2036..2380
                     /locus_tag="VLI90_10185"
     CDS             2036..2380
                     /locus_tag="VLI90_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14620.1"
                     /translation="MQQVKPDQPSGDPPSPLPYRSAAADRADHPRSRGSSFAIGCLAT
                     ILCIVLLASATVANATRNWAPYGWTAAGPALLAIGVAIGARRRDWGFFYGVLAWLILL
                     LSLVGLLFALCR"
     gene            complement(2542..3759)
                     /locus_tag="VLI90_10190"
     CDS             complement(2542..3759)
                     /locus_tag="VLI90_10190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSV14621.1"
                     /translation="MRALNTLIIGGGVAGASLACSLAEQGAGNGVGLVDPQLFGKWGS
                     SELNGGGVRCTFAEPINIRLSLASTRYYIEHADRFEFRQRGYLWMYDAPLWEHSRAFL
                     PLVRSFGLPVDEMTPAQTVERFPVLSDLSDLAGATFTPFDGRLSPHRLRLHYLNHAQA
                     GGIEFLDRWLVVAIDGERSPFRVTLRRMHPRSVKKALVDGAPAAGDELVIQAERVVNA
                     AGPWAARVAALYGRELPVTPMPRQVFLLRHPQINLEPLPFFIDYPQDIYFRHLERDRK
                     PLTLVSWSDPAELTRIDFVHHGDDYYRQHVQPRLVKRIAAMATAELAGGWTGHYELTP
                     DKSAIVGPVPGREGLFNLNGLSAHGVMQSRALGYAMATLLTSGHWPVELNLDELSESR
                     FAGSGRASEQMYV"
     gene            3883..>5407
                     /locus_tag="VLI90_10195"
     CDS             3883..>5407
                     /locus_tag="VLI90_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HSV14622.1"
                     /translation="MAPPRWLALILTLLLGAAAPAALPPPPADLVDWPMKWSPDDPGA
                     LSADNLIDKPAAKNGPITIRDAHFYSGDARVRFWGVNFAFSANFPTHEQADAVAHRLP
                     RFGINAVRFHHMDNQPFPNGIFADQSLEALSPEALDRLDYFIAALKQNGVYADINLHV
                     SRSYQHYHRTADGTDGPRVDKIVDLFDPDLIAAQKKYASDLLRHTNAYTHARYAEESA
                     VAVVEINNENSLFMWGARESLANLAEPYAAELKRQWNGWLLSRYGSRQKLQSAWSPLE
                     EPLGDNILRDGDFITLQPDHRTNGWNSEIQGSAMMSATFDRSGAGDGKARGAIINISA
                     VDGTNWHLQLTQPGLQLIKGRAYTVMLWARADKPMSMSVSVSEAHAPWQVLGLSAPVA
                     LQKTLQVYTLPFTAMASDDNARLCFAVGHETGTIYIPHVELKRGGRFGLRAEEDPGKD
                     SVMVESPSQSVTPRRSADWIEFLQQTEEKYYTGMREFLQKDVGVKCPITGTIGFGPLS
                     "
BASE COUNT         1050 a         1873 c         1566 g          918 t
ORIGIN      
        1 tctgtgggag atatccgcca tgagcgatga tccacttccc ccgtcgcaca tcgactaccg
       61 cccgcagccc gaaccgccgc cggggtggcc accgccgcgc acgcagcgca ttcaagggtt
      121 gcttgccggc tcactgctgt cgctcatcgt ttggccggtc gcgtggaagt tcggtcaaca
      181 aggcgcgacg ctggcttaca tcatcatcgg aatgattgct ctgaaagtca tcgcctccac
      241 cgtctgcatt gtttctccgc gctggcgacc gatgggagcc ggcatcttag cgtcgatggg
      301 cgtcggtttt ctgatcttct tcggcacgtg ctcgctcacc gcactccgtg gcatatagcg
      361 cgcgatgacg aggagaataa atacatgtcc gaccgcccct acctgttcta cgagctgacc
      421 aacagcctct gctccaaatg cctgcgcaag gttgaaacca aggtcgtcat tcaggacgac
      481 cgcgtttacc tgcacaagtg gtgccccgag cacggacgag aaaaggtgct gatttccacc
      541 gacgtcgagt attacaagct gtcgcgccaa acgctcaagc ccgggcagat gccgctgaaa
      601 tttcaaacgc cgatcaaata cggctgcccg ttcgactgcg gcctgtgccc cgaccacgag
      661 cagcactcgt gcctcacgct gctggagatc accgaccagt gcaacctcac ctgccccgtg
      721 tgctacagcg attcatcgcc gcggcggacg acgcaccgct cgctcgcgca gatcgagttc
      781 atgcttgact gcatcgtccg caacgaagcc gagccggacg tcgtgcagat cagcggcggc
      841 gaaccgacca tccacccgca gttcttcgac gtgctggacc tggccaagag ccggccgatc
      901 aagcacctga tgctgaacac caacggcatc cgcatcgcca acgaccccga cttcgcgcga
      961 cggctgaagg attacatgcc gggattcgag atctacctgc agttcgactc gatgaacccg
     1021 aaggtgttgc gcgagctgcg tggcgaagat ttgtccgata tccgccgccg cgcactcgat
     1081 cggctgaacg agcacaacat ctccacgacg ctggtggtca cgctgaagaa aggcttgaac
     1141 gacggcgaga tcggcgaaac catcgacttc gccctgaaac aaaaatgcat ccgcgggatc
     1201 acgttccagc ccatccaaca cgccggccgg ctcgaacatt tcgaccccgc gaccgatcgc
     1261 ctgacgctcg gcgaagtccg gcagcagatc ctcgcgcagt cgcctatctt taaaccgagc
     1321 gacgtcatcc ccgtcccctg ccaccccgac tgcctggcga tggcgtacgc gctgaagctg
     1381 gacggctgcg tcacgccact caccggcatg atcgacccgc agatcctcct gcacggcggc
     1441 gggaacacga tcgtctacga gaagcacgac tcgatccgcg gcgaactgtt ccgcctcttc
     1501 tccaccgccg ccagccccgg ctcctccgcc gcatcgctga agcagctgct ctgctgcctg
     1561 ccgatggtcg aggttgaagg cactgctatt acatacaaca acattttccg catcatcatc
     1621 atgcagttcc tggacgccca taatttcgac gtgagaagcg taaagaagag ctgcgtacac
     1681 atcgtgcacc ccgacgggcg aatcatcccg ttcgacacat tcaacctgtt ttatcgagat
     1741 gatcgtgagc gcatgctcga accgatgcgg cgggaacaac agctggtgac gctcggccga
     1801 ggcatgtcat cctaagcgca ggcgtactcg cggctcgact gagctcgcca aagtccggag
     1861 tcgaaggatc ttgctgagaa ggcaatgaag cgcgtctcac ggccgtgcgt ggtgttgggt
     1921 agtagcagag ccttattttg cgaagtccag cgattcgcgc acgagccgga gtgagcgtgt
     1981 cacggccacg agtttcgaag tcctgaaacg gccggcaaag cggcgggatg acgagatgca
     2041 acaggtcaag cctgatcaac ccagtggcga cccgccatca cccctgccct atcgctccgc
     2101 cgcagccgat cgcgctgacc acccccgcag ccgcggctct tcgttcgcca tcggttgcct
     2161 ggcgacgatc ctctgcatcg tgctattggc gagcgcgacg gtcgcaaacg ccacgcgcaa
     2221 ctgggcgccc tacgggtgga ccgccgccgg ccccgcactg ctggcgatcg gcgtcgccat
     2281 cggcgcccgc cgccgcgact ggggcttctt ctacggcgtc ctcgcgtggc tcatcctgct
     2341 cctaagcctc gtcggcctgc tgttcgcgct atgtcgatga gatgtggaag cggccccgcg
     2401 cccagccacc tatagcgacc gaagattcaa gaaacctccg cagcccacca cgccgggttc
     2461 ggaggtactc cgacgacccg gatgttttct cgcgtgaaga atccgagtcg tcgcgagtac
     2521 gctccgaacc cggcgttatg gtcaaacata catctgctca ctcgcccggc cgctgcctgc
     2581 aaatctcgac tcgctcagct catccaaatt caattccacc ggccagtgcc cgctcgtcag
     2641 caacgtcgcc atcgcataac ccagcgcgcg cgattgcatc acgccatgcg cgctcaggcc
     2701 attcagattg aacagcccct ctcgccccgg cacaggtccg acgatcgccg acttatccgg
     2761 cgtcagctcg taatgccccg tccacccgcc ggccagttcc gccgtcgcca tcgcggcgat
     2821 gcgcttgacc agccgcggtt gcacgtgctg gcgatagtag tcatccccat gatgcacgaa
     2881 gtcgatccgc gtcagttccg ccggatcgct ccagctgacc agcgtcagcg gcttgcggtc
     2941 gcgctccagg tggcggaaat aaatgtcctg cgggtaatcg atgaagaatg gcagcggctc
     3001 gaggttgatc tgcggatgcc gcagcaaaaa cacctgccgc ggcatcggtg tgaccggcaa
     3061 ttcgcgccca tacaacgccg ccacccgagc cgcccagggc cccgcggcat tgaccacgcg
     3121 ctccgcctgg atcaccaact catcccccgc cgccggcgcg ccgtccacca gcgctttctt
     3181 caccgagcgc ggatgcatcc gccgcagcgt cacgcggaac ggcgatcgct ccccatcgat
     3241 ggcgaccacc agccagcggt cgaggaattc gatgccgccg gcttgcgcgt ggttcaggta
     3301 atgcagccgc aagcgatgcg gcgacagccg cccgtcgaac ggtgtgaacg tcgcgccggc
     3361 caagtcggaa agatcgctga ggacgggaaa ccgctcgacc gtctgggccg gcgtcatctc
     3421 atccaccggc aaaccgaacg accgcaccag cggcaggaac gcgcgcgagt gctcccacag
     3481 cggcgcatcg tacatccaca gataaccgcg ctggcggaat tcgaatcggt ccgcgtgctc
     3541 gatatagtac cgcgtggagg ccaacgacag gcggatgttg atcggctcgg cgaacgtgca
     3601 gcgcacgccg ccgccgttta gctcgctcga tccccatttg ccgaacagct gcggatcgac
     3661 caggccaaca ccgttgccgg cgccttgttc ggcaaggctg caggcgagcg aagcgcccgc
     3721 cacgccgccg ccgatgatca acgtgttcag cgctcgcatg tcatctcaat atgagtcgcc
     3781 gccgcttgcg gcttcgcagg ccacgccggc tggattgcga agccagcaag tggcggcgcg
     3841 gtcatcctac caccatccga aactggattt atactgccac ccatggcacc accgcgatgg
     3901 cttgcactca ttctcacgct gctactgggc gccgcggcgc ccgccgccct cccgccgcca
     3961 ccggcggatc tggtcgattg gccgatgaag tggtcgccgg acgatccggg cgcactgtct
     4021 gccgacaacc tgatcgacaa gcccgccgcg aagaacggcc ccatcaccat ccgcgacgcc
     4081 cacttttaca gcggtgacgc ccgggtgcgc ttctgggggg tcaacttcgc gttcagcgcg
     4141 aacttcccca cgcacgagca ggccgacgcc gtcgcgcatc gcctgccgcg cttcggcatc
     4201 aacgccgtgc ggttccatca catggacaac cagccgttcc ccaacggcat cttcgccgac
     4261 caatcactcg aagcgctgtc gccggaggcc ctcgaccggc tcgattactt catcgccgca
     4321 ctgaagcaga acggcgtcta cgccgacatc aacctgcacg tctcgcggtc gtaccagcat
     4381 taccatcgca cggctgacgg cacggatggc ccgcgcgtgg acaagatcgt ggacttgttc
     4441 gaccccgatc tcatcgccgc tcaaaagaaa tacgccagcg acctgctccg ccacaccaac
     4501 gcctacaccc acgcgcgcta cgccgaagaa tcggcggtcg ccgtcgtcga gatcaacaac
     4561 gagaactcgc tgttcatgtg gggcgcgcgc gaatcgctgg cgaatttggc cgagccctat
     4621 gcagcggagt taaagcggca atggaacggc tggctgctgt cgcgctacgg gtcgcggcag
     4681 aaactgcaaa gcgcctggtc gccgctggaa gagccgctcg gcgacaacat cctgcgcgac
     4741 ggcgacttca tcacgctgca acccgaccac cggacgaatg gctggaacag cgagatccag
     4801 ggttcggcca tgatgtcggc gacgttcgac cgcagcggcg ccggcgatgg caaggcgcgc
     4861 ggggcgatca tcaacatcag cgcggtcgat ggcaccaatt ggcatctgca actgacgcag
     4921 cccggccttc agctgattaa gggccgggcc tataccgtga tgctctgggc tcgcgccgac
     4981 aagccgatgt cgatgagcgt cagcgtttcc gaagcgcacg cgccttggca ggtgcttggg
     5041 ctatccgcgc cggtcgcgct gcagaaaacg ttgcaggtct acaccctgcc cttcaccgcg
     5101 atggccagcg atgacaacgc ccggctttgc ttcgcggtgg gtcacgagac cggcacgatc
     5161 tacatcccgc acgtcgagct caaacgcggc ggacggttcg gcctgcgcgc cgaggaagac
     5221 ccgggcaagg acagcgtgat ggtcgaatcg ccgtcgcagt cggtcacgcc ccggcgatcg
     5281 gccgactgga tcgaattcct gcagcagacc gaggagaaat attacaccgg catgcgcgag
     5341 ttcctgcaga aggacgttgg cgtgaaatgc ccgatcaccg gcacgatcgg cttcggcccg
     5401 ctgagca
//
LOCUS       DATIMH010000589         6192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5053588, whole genome shotgun sequence.
ACCESSION   DATIMH010000589 DATIMH010000000
VERSION     DATIMH010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5053588"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2390)
                     /locus_tag="VLI90_10200"
     CDS             complement(<1..2390)
                     /locus_tag="VLI90_10200"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV14623.1"
                     /translation="MAFPRARGRVRLQFLNAISHGIEQLERRTLMAAGALDTSFAGSG
                     KDSLAAPPSTTINVGLDPTPVGLAIGPDGKAIISANGLDPSGGKFVELIRLLPNGSRD
                     VTFGSAGAVTLFNTTVTKVAILNDGRILIGGSILARLNNNGAFDSTFGGLGFGFRGVS
                     TPVVTGMALQSDGHILLAGQSGQSAAVWRYNPDGTTDGSYGAGGVRPLVFVEEDNSLI
                     ATAAGGIALDPQGRVVAGVSVTGPGTDTGYGVARINTNGSIDTKFGTGGLTQIDDAAP
                     AAPAVAVASTGTIYLAATQGIASATSHGLLMVMSADGKKFAVRTTGLVPTTSAALDVQ
                     VQADNKPIVVGWERDGGGNGNKKIIAQRFQAGSPTLPVAAALDRTFNSTGSIEVDYNA
                     GGSSEKTFGFGDLGWSLRLSPNQKIFIAGTSVGASGSQFTVTRLLPDSATLNSFGTIS
                     GTTFFDPNGNGVLESPDVPISGIETFLDLNGNGTFDGGDVRAFGNIAGAYQFIGLKAG
                     TYQVRQHAGGNIKTTIPDNGLRTVTLASGGSVGNVNFAISVAGSISGFIFEDLNGDGL
                     GEINEPVLPGFDCYLDLNNNGVYDAASEPRTIAGADGIYAFGGLNAGTYVVRQATPAG
                     WTLTGPPAQAWTITLAQNEHRNNVNFADRAIAPGAHLDADGTLVVVGSAADDNIVVNQ
                     QVALGAAAGTVFVTVDANQQQFTTPAVKRIYIDGRDGNDVITDSGTDFTAGADGGIGI
                     LSTLIGGNGNDTLRVTLNDTQSNRSASDFVDGGAGDDTLFNDSNGNITMHGGDGNDT"
     gene            complement(2647..5187)
                     /locus_tag="VLI90_10205"
     CDS             complement(2647..5187)
                     /locus_tag="VLI90_10205"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HSV14624.1"
                     /translation="MADDASMSNQDPATALPAAAAAAIAAANGGYNEAMIRVLDNVTH
                     VRTRPGMYIGGYNARGLHHLVYEIVDNSIDEALAGYCKSINVKINADGSCTVTDDGRG
                     VPVGIHPTEGIPTVEVVFATLGAGGKFEHNDDSAYKTSGGLHGVGASVVNFVSEWMEI
                     EVSREGQVHHMEFSRGIKSQDLKVIGKSGKSGTKVTFKPDPTLFPDVNFVHETLLNRL
                     RELAFLNAGVEIVFEDERLGKRDAFKFEKGLTEYVQFLNEGKQALSPVISFTREDAEQ
                     KLVVEVALQYNDGYNETVLTFANNINNHDGGTHLSGFRTGLTNTILRYAERTNLLKDV
                     RPSGDDLREGLVAVISVKIPEPSFESQTKDKLLNVEVEGFMNSSVSERLGSFFEENPK
                     DARAIFEKGMLAAEAREAARKARELTRRKNSLEGGSLPGKLADCRSKSNEDTELFLVE
                     GDSAGGSAKQGRDSNIQAILALKGKILNVEKANLVKMLGHEEIRTIISAMGCGIREEF
                     NAEKRRYGKIIIMTDADVDGSHIRTLLLTFFFRHMQELIRAGRVYVAQPPLYQVTRRK
                     KSEYVLNERTMRQTLTALGTDGTSLVIRDAEGVNEVRRVSGEELTRVVELLNKLEELV
                     KVVQRRGIDFAELLDKRDSSGALPKYHIVIDGTDYFKHDAKQRDALLQEHKLWAEEET
                     LPETDSAEPTAKPPTFQRLQKNQELHEMKELEKLFTQLEAYDISIDDYYLTQEESVSG
                     EKLATKYVLLNEEKQINIAGVGQILSEIHRLAKQGIEVKRFKGLGEMNSEELWETTLD
                     PANRVLLRVTLNEAAEAERMFSVLMGEDVERRRQYIEEHALEVKNLDV"
     gene            5536..>6192
                     /gene="rsgA"
                     /locus_tag="VLI90_10210"
     CDS             5536..>6192
                     /gene="rsgA"
                     /locus_tag="VLI90_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HSV14625.1"
                     /translation="MPARHRKPRDKSLTERYRAGDFDAERVEQEEADPSAERFSARSK
                     NAQQNKILRTSLMRAAEQAIAIDPETLPIALVTQVYSLYVQIEHADRVYLAVVRKTLT
                     KVSDTQLVVGDQVRFRDVGATDESGQPEAVIEQILPRRTVLTRADSFKGNIQHPIVAN
                     AEQMLIVASVKLPAVKWGLVDRMLIAARSGGLVPIVCLNKMDLSVDSAPEAGAERATD
                     T"
BASE COUNT         1044 a         2042 c         1865 g         1241 t
ORIGIN      
        1 gtgtcattac cgtcgccgcc gtgcatggtg atgttgccgt tgctgtcgtt gaagagcgtg
       61 tcgtcgcctg cgccaccgtc gacgaaatcg gaagccgacc gattgctctg cgtatcgttc
      121 aacgtcacgc gcaaggtgtc gttgccgttg ccgccgatca atgtgctgag gatgccgatg
      181 cccccgtcgg cgcccgccgt gaagtcagtg ccgctgtcgg tgattacgtc gttcccgtcg
      241 cggccgtcga tgtagatccg cttcaccgcc ggcgtggtga actgctgttg gttggcatcc
      301 accgtgacga acacggtgcc cgccgccgcg cccaacgcga cctgttgatt gacgacgatg
      361 ttgtcatccg ccgccgaccc gacgacgacc agcgtgccat cggcatccag gtgcgcgccc
      421 ggcgcgatcg cgcgatcggc gaagttgacg ttgttgcgat gttcgttctg cgccagcgtg
      481 atcgtccatg cttgcgccgg cgggcccgtc agcgtccacc ccgcgggcgt cgcctggcga
      541 acgacatagg tgccggcgtt caacccgccg aacgcgtaga tgccatcagc gccggcgatc
      601 gtccgcggct cgctcgccgc gtcgtagacg ccgttattgt ttaaatcgag atagcaatcg
      661 aagcccggca gcaccggttc gttgatctcg cccagcccgt cgccgttgag gtcttcgaaa
      721 ataaaaccgg agatcgaccc ggcgacactg atggcgaagt tcacgttgcc caccgaccca
      781 ccgctggcca gcgtcaccgt gcgcaggccg ttgtcgggaa tcgtcgtctt gatgttcccg
      841 cccgcgtgct gccgcacttg atacgtgccg gccttaaggc cgatgaattg gtacgcaccg
      901 gcgatattgc cgaaggcgcg cacgtcgccg ccgtcgaacg tgccgttgcc gttcaggtcc
      961 aggaacgttt cgatgccgga gatgggcacg tcgggacttt caagcacgcc gttgccgttg
     1021 gggtcgaaga acgtcgtgcc gctgatcgtg ccgaacgagt tgagcgtcgc gctatcgggc
     1081 agcaggcggg tcacggtgaa ctgcgatccg ctggccccga ccgatgtgcc ggcgataaag
     1141 atcttctggt tcggcgacag ccgaagggac cagccgaggt cgccgaagcc gaacgtcttc
     1201 tcgctcgatc cccccgcgtt gtaatcgacc tcaatggaac cggtgctgtt gaacgttcga
     1261 tccagtgccg ccgccaccgg cagcgttggg ctgccggcct ggaatcgctg ggcgatgatc
     1321 ttcttatttc cgttgccgcc gccgtcgcgt tcccagccga ccacgatcgg cttgttgtcc
     1381 gcctgcacct gcacgtccag cgcggccgac gtcgtcggca ccaggccggt cgttcgcacc
     1441 gcgaacttct ttccgtccgc cgacatcacc atcagcaatc cgtggctggt ggcgctggcg
     1501 atcccctgcg tggccgccag gtaaatcgtg ccggtgctgg caacggcaac cgccggcgcc
     1561 gccggggcgg catcgtcgat ctgcgtgagc ccgccggtgc cgaacttcgt gtcgatgctg
     1621 ccgttcgtgt tgatgcgcgc gacgccatag ccggtatcag tgccaggacc ggtcacgctg
     1681 acgccggcga cgacgcggcc ctgcgggtcc agcgcgatgc cgcccgccgc cgtcgcgatc
     1741 agcgaattgt cttcttcgac gaacaccagc gggcgcacgc cgccggcgcc gtagctgccg
     1801 tcggtcgttc catccgggtt gtagcgccac acggccgcgg attggccgct ttggccagcg
     1861 agcaagatgt gcccatcgct ctgcagcgcc atcccggtga cgactggcgt cgagacgccg
     1921 cggaatccga aaccgagtcc gccgaaggtc gagtcgaacg cgccgttgtt gttcaggcgc
     1981 gccaggatcg acccgccgat caggatgcga ccatcgttca ggatcgcgac tttggtgacc
     2041 gttgtgttga agagcgtgac ggcgccggcg ctgccgaagg tcacgtcgcg cgagccgttg
     2101 ggcaggaggc gaatcagctc gacgaatttg ccgccgcttg ggtccagtcc gttggcggaa
     2161 atgatcgcct tgccatcagg gccgatcgcc aatccgaccg gcgtcggatc gagtccaacg
     2221 ttgatggtgg tcgaaggcgg agccgccagc gaatccttgc ccgacccggc gaagctggtg
     2281 tccagcgcgc ccgcggccat cagggttcgc cgttccagct gttcgatgcc atgcgaaatc
     2341 gcgttgagga actgcagccg cactcgcccc cgagcccgcg ggaaagccat ggtgtcgccc
     2401 tcacatcaag gatgtcgtgc aaagcggatt tttagcccga agaaccccca gccggcgcct
     2461 ccaccggcaa ctcgtcagat tacggcgtgg caacgcgtgg agcaacaaaa ttatgggagt
     2521 gggaagatag gcgagttgta gcgggcaaat atgccaacca cgccgggttc ggagcgtact
     2581 cgcgacgacc cggatccttc gcgcgaagaa tccgggtcgt cgcgagtacc tccgaacccg
     2641 gcgtgttcac acatccaaat ttttcacttc cagcgcgtgc tcctcgatgt actgccggcg
     2701 gcgttccacg tcctcgccca tcaacacgct gaacatacgc tcggcttcgg cggcttcgtt
     2761 cagcgtcacc cgcagcagca cgcggttcgc cgggtccagc gtcgtctccc acagctcttc
     2821 gctgttcatc tcgcccaggc ccttgaatcg cttgacctcg atgccctgct tggccagccg
     2881 atggatctcc gacaagatct gcccgacgcc ggcaatgttg atctgctttt cttcgttcag
     2941 cagcacgtac ttcgtcgcca gcttctcgcc gctgaccgac tcttcctgcg tcagataata
     3001 gtcatcgatg ctgatgtcat atgcttccag ctgcgtgaag agtttttcca actccttcat
     3061 ctcgtgcagt tcctggttct tctgcagccg ctggaacgtc ggcggcttcg ctgtcggttc
     3121 ggcgctgtct gtttccggca gcgtttcctc ctcggcccac agcttgtgct cctgcagcag
     3181 cgcatcgcgc tgcttggcgt cgtgcttaaa gtaatcggtg ccgtcgatca cgatgtgata
     3241 cttcggcagc gcgccgctgc tgtcccgctt gtcgagcagc tcggcaaagt cgatcccgcg
     3301 gcgctgcacc accttcacca gctcttccag cttgttcagc aattcgacca cgcgcgtcag
     3361 ctcttcaccg ctcacgcggc gcacttcgtt caccccttcg gcatcacgga tgaccagtga
     3421 cgtgccatcg gtccccagcg cggtgagcgt ctgccgcatc gtccgctcgt tcagcacgta
     3481 ctcgctcttc ttccgccgcg tcacctgata cagcggcggc tgggcgacgt acacccggcc
     3541 ggcgcggatc agctcctgca tgtgccggaa gaaaaacgtc agcagcagcg tgcgaatgtg
     3601 cgaaccgtcc acgtcggcgt cggtcatgat gatgatcttg ccgtaccgcc gcttctctgc
     3661 gttgaattct tcgcggatgc cgcagcccat cgcgctgatg atcgtgcgga tttcctcgtg
     3721 gcccagcatc ttcaccaggt tcgccttctc gacgttcagg atcttaccct tcagcgccag
     3781 gatggcctgg atgttcgagt cacgcccttg cttggccgac ccaccggcgg agtcaccttc
     3841 gaccaggaac agttccgtat cttcgttcga cttgctgcgg caatccgcca gtttccccgg
     3901 cagcgaaccg ccttccaaac tgttcttgcg ccgcgtcagt tcgcgcgcct tgcgcgccgc
     3961 ctcgcgcgcc tcggcggcca gcatgccctt ctcgaagatc gccctcgcgt ccttcgggtt
     4021 ttcttcgaag aacgaaccga gccgttcgct gacggaggaa ttcatgaacc cctccacctc
     4081 aacgttcagc aacttgtcct tcgtctgcga ttcgaacgac ggctcgggga tcttcaccga
     4141 gatgaccgcg accaatcctt cgcgcaggtc gtcaccactg ggacgaacat ccttcagaag
     4201 gttcgtccgc tctgcgtagc gcaggatcgt attggtcaac cccgtgcgga acccgctcag
     4261 gtgcgtgccg ccgtcgtggt tgttgatgtt gttcgcgaac gtcaggacgg tttcgttgta
     4321 gccgtcgttg tattgcagcg cgacttcgac gaccagcttc tgctcggcgt cttcccgcgt
     4381 gaagctgatc accggcgaca acgcctgctt accttcgttc aggaactgca cgtattcggt
     4441 cagccctttt tcgaacttaa acgcatcgcg cttgcccagc cgctcgtctt cgaaaacgat
     4501 ctcgacgcca gcgttcaaaa acgccagctc gcgcaggcgg ttcagcagcg tttcgtgcac
     4561 gaaattcaca tcgggaaaca gcgtcgggtc cggcttgaag gtcaccttcg tgccgctctt
     4621 gccgctcttg ccgatgacct tcaggtcctg gctcttgatc ccgcgggaga attccatgtg
     4681 atggacttgc ccctcgcgcg agacttcgat ctccatccat tcgctgacga agttgacgac
     4741 ggacgcgccc acgccgtgca gcccgccgct ggtcttgtag gccgaatcgt cgttgtgctc
     4801 gaacttgccg ccggcaccga gcgtggcgaa gacgacttca acggtgggaa tgccttctgt
     4861 cgggtggatg ccgaccggca cgccgcggcc gtcgtcggtg acggtgcacg aaccgtcggc
     4921 gttgattttc acattgatgc ttttgcagta cccggccagc gcttcgtcga tcgagttatc
     4981 gacgatttca taaacaagat gatgcaaccc gcgggcgttg tacccgccga tgtacatgcc
     5041 gggccgcgtg cgcacgtgcg tgacgttgtc gagcacgcgg atcatcgctt cgttgtagcc
     5101 cccgttggcg gcggcaatcg ccgcggcggc agcggcgggc aacgcggtgg cgggatcttg
     5161 attggacatc gaggcgtcgt cggccattag tcttgctcct gtcacacgcg acattggctt
     5221 gccaaggaca agcgtgcgtc gcgcggctga tgattcgatc gaccgcggtt ccaaaccgcg
     5281 ggcagaggcg ttcgcgagat gttgctcaaa tcacccccga atcgggggac gtggaacaac
     5341 gatatcatta tcgctaaacc ccggataaat actacgtttt cggcaggaaa aaggtcggtg
     5401 cttaagcggg ctcagacgga gcgaaatcag agatgaaaaa cggccaatcc ctgcagcacg
     5461 ggcacctggc cggtgtccaa gctgcgcggc acaggcgaga cgcccgtgct acttcaccgt
     5521 agactccccg catcgatgcc cgctcgtcac cgtaagccac gcgacaagag tttgaccgag
     5581 cgctaccgcg ccggcgactt tgatgctgaa cgcgtggagc aggaagaagc cgatccatcc
     5641 gccgagcgtt tttcggcgcg gtcgaaaaac gcccagcaga acaagatcct ccgcacctcc
     5701 ctgatgcgtg ctgccgagca ggcgatcgcg atcgatcccg agacgctgcc catcgcgctg
     5761 gtcacgcagg tgtattcgct gtacgtccag atcgagcacg ccgaccgcgt ttatctagcc
     5821 gtcgtccgca agacgctgac gaaggtgtcc gacacgcagc tggtcgtcgg cgaccaggtg
     5881 cgcttccgcg acgttggcgc caccgacgaa tccggccaac ccgaagccgt catcgagcag
     5941 atcctcccgc ggcggaccgt cctcacccgc gctgacagct tcaaagggaa cattcagcac
     6001 ccgatcgtcg cgaacgccga gcagatgctg atcgtcgcca gcgtgaagct gccggcggtg
     6061 aagtggggat tagtcgatcg catgctgatc gccgcccgca gcggtgggct ggttccgatc
     6121 gtctgcctga acaagatgga cctgagcgtg gattcggcgc ccgaagcggg tgccgagcgc
     6181 gcgacggaca cg
//
LOCUS       DATIMH010000590         4853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_721756, whole genome shotgun sequence.
ACCESSION   DATIMH010000590 DATIMH010000000
VERSION     DATIMH010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721756"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..415)
                     /locus_tag="VLI90_10215"
     CDS             complement(128..415)
                     /locus_tag="VLI90_10215"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HSV14626.1"
                     /translation="MLARITAVRPTGDYRLHLTFADGRAGEIDLRGQIVGRGGVFQIL
                     EDRAFFNQVRVDTEAATIAWPNEVDLDPDVLYSLVTGIPVADLVQAAMSHD"
     gene            complement(361..681)
                     /locus_tag="VLI90_10220"
                     /pseudo
     CDS             complement(361..681)
                     /locus_tag="VLI90_10220"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
     gene            complement(697..1902)
                     /gene="carA"
                     /locus_tag="VLI90_10225"
     CDS             complement(697..1902)
                     /gene="carA"
                     /locus_tag="VLI90_10225"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HSV14627.1"
                     /translation="MNHDENPRIAKVALEDGTVFAGRAFGAAGTSEGEVVFNTSMTGY
                     QEILTDPSYKGQIVTMTYPLIGNYGINRQDVESKRPHVAGFIIKELSPVYSNYRADLS
                     LDEYLKQNNIIGIEGIDTRALVRKLRISGAMRGILSTELLDDAQLVDKAKHAAGMIGA
                     DWVQAVKPTHSYAWDEDQGDWGQGKVERGDGLHVVALDCGAKSNILRHLAERGVKVTV
                     LPPDITAEEILSHHPDGLFVSNGPGDPAALDYAVKPIKAVLDKVPTFGICLGHQLLGR
                     AIGADTYKLKFGHRGGNQPVKNLDTGRVEITSQNHGFAVDQKSLEQHGGVVTHLNLND
                     NTVEGFRHGKLPVFSVQYHPEASPGPHDAAYLFDAFIEMMKTKKAPTGKRLAELQALR
                     DTPNAIAGK"
     gene            complement(2059..2829)
                     /locus_tag="VLI90_10230"
     CDS             complement(2059..2829)
                     /locus_tag="VLI90_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HSV14628.1"
                     /translation="MLRPNQILPVFAVCLLPFICASSSFAAITHRYSFNDPAAPGKDS
                     VGNVDAKLKGPDAKVADGKLTLKNEDKNSDDPQLSYAKFDEPIIPKTGSASIAVWFTA
                     KDAGQYARIFNFSDKDGSEGRAFIYFTPRTSTDESRGAITATDAASKTAIDNPSLDDG
                     KPHMVAIVIDGVSKKLHVYIDGTEPKPAQDLGDNTLDKVKPTNNWLGRSSFDQDAGLT
                     GSIDELRTYDHALTADEVSESFKSGADTLPPSAAAPKP"
     gene            complement(2957..4705)
                     /locus_tag="VLI90_10235"
     CDS             complement(2957..4705)
                     /locus_tag="VLI90_10235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV14629.1"
                     /translation="MKPTVPARRGVLAVCAIGLLLLLPFINKPFHSDDPFFLWPAIGI
                     LKHPANFYSDSINWEGREQHFSDFCQNPPLTSYAIAAVASLAGFSERALHLSFLVFNL
                     STIVGVYLLAQHFGCRRPALAVVLMLLAPAVLVSMTSIMCEPVFLCFWTWSVLLWEWG
                     SSSRRPWLLPLAGVAIAAAILSKYLCVCLLPLLIAHAILRPAPGWRTRLFQFLSMLIP
                     AAALAVYEWYCVRLYGHGLFTAAMRFGAQIHEAVKIPKPLAALDTLAFLGGGFFSVAL
                     INCFAVRPRMRWLCVLAIPLIALLAAHYVRPPRGWTGAPPIPGGANVIDASDPSAPIS
                     TGRWYYIEYGLLGAAGLLILVACVQWLLFHVKSSRDDATENWRADLFLLLWIGGIIVF
                     AAFINWSINARTLVPMAPALCILVVRMIDGLSKRALRVVQLSLIAAAVLAIAATAADY
                     RVATACRDAADQIDAFFAPRPDQTIWFAGHSGWQYYMQQHGARPLVQYATDVRPGDIV
                     VYPVHHFGASPGGHGFQYIRTFTIPHPGYVATMQTDLGAEFYCSFGQGLPFIIGPVQP
                     ESFIALRATPATRIER"
BASE COUNT          931 a         1310 c         1684 g          928 t
ORIGIN      
        1 aatcgagcgg cgacggcaat gaaaacgaca atggtggcaa gtccggcaag gtacccatag
       61 atcggcagga tgatgcagcg ccttgtactt tcgcgagccc aggcgagtcc ggtaatggcg
      121 gcgaatttca atcatgcgac atggcggctt gaacgagatc cgcgacggga atgcctgtca
      181 cgagcgaata caacacatcg ggatccagat cgacctcgtt tggccaagcg atcgttgccg
      241 cttccgtatc gactcggacc tggttgaaga aggcgcggtc ctcgaggatc tggaacacgc
      301 cgccgcggcc gacgatctga ccgcgcaaat caatttcacc ggcgcgaccg tcggcgaagg
      361 tcagatgcag gcggtagtcg ccagtcggtc gcacagcggt gattcgagcg agcattggca
      421 caccccaaat gagcggagcg attttgccgg ctgctgagta gcgcgaagga gcagccaatt
      481 ctcggccaat tcagcctgat gaagcgcagc ccattctaac gccatcgaca atgcgcgggc
      541 aggcaaatgt ccatcaagct atcgcaccgg gtcgattcca acgatggcca tcgcgctgcc
      601 gtagatcgcg tgaaaatgcg gcgggttgcg gtcattgaaa tacatgcgga tgatgccaaa
      661 gaatcggctg atttccggca tcaaatcacc tgcaggtcat ttgccagcga tggcgtttgg
      721 agtgtcgcgc agcgcctgta actcggccag tcgctttcct gtcggcgcct tcttcgtctt
      781 catcatctcg atgaacgcgt cgaagagata cgccgcgtcg tgggggccgg ggctggcttc
      841 ggggtggtat tggacgctga agaccggcag cttgccgtgg cggaatcctt ccaccgtgtt
      901 gtcgttcagg ttcaggtgcg tgaccacgcc gccgtgttgt tccaggctct tttgatcgac
      961 ggcgaagcca tggttttggc tggtgatttc gacccggccc gtgtcgaggt tcttcaccgg
     1021 ctggttcccg ccgcggtggc cgaattttag tttgtaggtg tcggcgccga tcgcgcggcc
     1081 gagcagttga tgaccgaggc agatgccgaa ggtgggcacc ttgtccagca ccgccttgat
     1141 cggtttgacg gcgtaatcga gggcagcggg atcgccgggg ccgttgctga cgaacaggcc
     1201 gtcggggtgg tggctgagga tttcttcggc ggttatgtcg gggggcagca ccgtgacttt
     1261 gacgccgcgt tcggcgaggt ggcggaggat gttgctcttg gcgccgcaat cgagggcgac
     1321 gacgtggagc ccgtcgccgc gttcgacttt gccttgtccc cagtctccct ggtcttcgtc
     1381 ccacgcatag ctatgcgtgg gctttaccgc ctgcacccag tcggcgccga tcattcccgc
     1441 ggcgtgcttg gctttgtcca ccagctgcgc gtcgtcgagc agttctgtgg agaggatgcc
     1501 gcgcatcgca ccgctgatgc ggagctttcg cacgagcgcg cgcgtgtcaa tcccttcgat
     1561 gccgatgatg ttgttctgct tcaggtattc gtcgaggctc aggtcggcgc gatagttcga
     1621 atagaccggc gacagttcct tgatgatgaa ccccgcgacg tgcgggcgtt tgctttcgac
     1681 gtcctggcgg ttgatgccgt agttgccgat cagcgggtag gtcatcgtca cgatctgccc
     1741 cttgtaggac ggatcggtga ggatctcctg atagccggtc atcgaggtgt tgaagacgac
     1801 ttcaccttcg ctggtgccgg cggcgccgaa ggcgcggccg gcgaagacgg tgccgtcttc
     1861 gagagcgacc ttggcaattc gtggattttc gtcatggttc atcgcggcgg gattatacgc
     1921 gggcgggatg cgaattcacg cccgggcgat tggcggctac ggaaggattc attgtccggt
     1981 cccctctccc ggtactccgg gagagggggg taagaccaga cggcgcctgc ggagcgaaga
     2041 taaataaatg gaaacaattc acggcttggg ggcggcggca gacggcggca gcgtatcggc
     2101 gccggacttg aacgactcgc tcacttcgtc cgcggtcagc gcgtggtcgt atgttcgcag
     2161 ctcgtcgatc gagccggtga ggcctgcatc ctgatcgaac gatgatcggc cgagccaatt
     2221 gttcgtcggc ttcaccttgt ccagcgtgtt gtcgccgagg tcctgggcgg gcttgggttc
     2281 cgtgccgtcg atgtagacgt gcagcttctt tgagacgccg tcgatcacga tggcgaccat
     2341 gtgcggcttc ccgtcgtcga ggctcggatt gtcgatcgcg gttttggagg cggcgtcggt
     2401 ggcggtgatg gcgccacgcg attcatcggt gctggtccgc ggggtgaagt agatgaaggc
     2461 gcggccttcg gagccgtcct tgtcgctgaa gttgaagatg cgagcgtatt ggccggcgtc
     2521 cttggcggtg aaccagacgg cgatgctggc gctgccggtc ttgggaatga tcggctcgtc
     2581 aaacttcgcg taggacagct ggggatcgtc gctgttctta tcttcgttct tcagcgtgag
     2641 ctttccatcg gcgaccttgg cgtcgggacc tttgagcttg gcgtcgacgt tgccgacgga
     2701 atcttttcct ggcgcggcgg gatcattaaa actgtagcga tgcgtgatgg cggcgaagga
     2761 actgctcgcg cagatgaatg gaagaagaca gacggcgaag accggtagga tctggtttgg
     2821 tcgcagcatg aagtctcctc tgtatagaag gaaattgaat cgtttcccga tctcgctgcc
     2881 ggcccaaggg accgcgaagc gcagaccgtc gcgccgaaca ttatcgcgca acagggttgt
     2941 acacgcgaat tcctgattaa cgctcgattc gtgtcgccgg tgtggcgcgc agggcgatga
     3001 aggattccgg ctgcacgggt ccaatgatga acggcagtcc ttggccgaac gagcagtaga
     3061 attctgcgcc gagatcggtc tgcatggtcg caacgtagcc ggggtggggg atggtgaagg
     3121 tgcggatgta ttggaaacca tggccgccgg gggaggcgcc gaagtggtgg acggggtaga
     3181 cgacgatgtc gccggggcgg acgtcggtgg cgtattgcac aagcgggcgg gcaccgtgct
     3241 gctgcatgta gtactgccag ccggagtgac cggcgaacca gatggtttga tcgggacgcg
     3301 gggcgaaaaa ggcgtcgatc tgatcggcgg cgtcgcggca ggcggtggcg acgcggtagt
     3361 cggcggcggt tgccgcgatc gcgagaacgg cggcggcgat cagcgagagt tgtacgaccc
     3421 gcaacgcgcg cttggacaag ccatcgatca ttcgcacgac gagaatgcac agagccggtg
     3481 ccatcggcac cagcgttcgc gcgttgatcg accagttgat gaacgcggcg aagacgatga
     3541 tgccgccgat ccacagcagg aggaagagat cggcacgcca gttttcggta gcatcgtcgc
     3601 ggcttgattt cacatggaat agcagccact gcacgcaggc gacgaggatg agaagaccag
     3661 cggcgccgag caagccatat tcgatgtagt accaccgccc ggtgctgatc ggagcgctgg
     3721 ggtcggaggc gtcgatgacg tttgcgccac cgggaattgg cggcgcgccg gtccagccgc
     3781 gcgggggtcg gacataatgc gcggcgagta atgcgatgag tgggatcgca agcacgcaca
     3841 gccaacgcat gcgggggcgg acggcgaagc agttgatcaa tgcaaccgag aagaatccgc
     3901 cgccgaggaa tgcgagcgtg tcgagcgcgg ccaggggttt gggaatcttg acggcctcgt
     3961 ggatctgcgc accgaagcgc atggcggcgg tgaagaggcc gtggccgtag aggcgaacgc
     4021 agtaccattc gtacaccgcc agcgccgcgg cagggatgag catcgacagg aattgaaaca
     4081 ggcgcgttcg ccagccgggc gcggggcgaa gaatggcgtg cgcgatcagc agcggcagga
     4141 ggcaaacgca gaggtacttg gacaagatcg ccgcggcgat agcgacgccc gcgagtggca
     4201 gcagccaggg tcgacggctg ctggatcccc attcccagag cagcacggac cacgtccaga
     4261 agcagaggaa gaccggctcg cacatgatgc tggtcatcga cacgagcacc gcgggcgcga
     4321 gcagcatgag cacgacggca agcgccggcc ggcggcaacc gaagtgttga gcgagcagat
     4381 agacgccgac gatcgtcgag agattgaaga cgagaaacga caagtgcagg gcgcgctcgc
     4441 tgaagccggc caagctggcg acggcggcaa tggcgtatga cgtgagcggt ggattctggc
     4501 aaaaatcgct gaagtgctgc tcgcggcctt cccagttgat cgagtcggag taaaaatttg
     4561 ccggatgctt aagaatcccg atggcgggcc agaggaagaa cgggtcgtcg ctgtgaaacg
     4621 gtttgttgat gaaggggaga agcagcagca acccgatggc gcaaacggcg aggacgccgc
     4681 ggcgcgcagg caccgttggt ttcatcgcgt gatcaggcgc tttcgattcc attaagcccg
     4741 ccaggagatt gggcggattg actagagtaa cagcgggctg gttgcgcgca aaaaagaacc
     4801 ccgatctttc gaagaccggg gttcttgttt aatgctggca gtgacctact ttc
//
LOCUS       DATIMH010000591         8463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_141858, whole genome shotgun sequence.
ACCESSION   DATIMH010000591 DATIMH010000000
VERSION     DATIMH010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141858"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VLI90_10240"
     CDS             <1..907
                     /locus_tag="VLI90_10240"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV14630.1"
                     /translation="DVTNNSAFVINANTTASNVSGAGFTSVNATKFFNTTNFTQSSGL
                     INNGTTQINGNGTVGLIAGSGALTIGNGATTNTLALAHNSGGSTQASITINAGAKLDI
                     NNNHMFFTTTPLTTVRGYVISGAIFSALSDTTHAVGYASVAGPQVEVMWTLKGDADLS
                     GTVDVGDLGKLATSYGAVTTGATWFQGDFTYDGTVDVADLGALATNYGTTLVGGAGDA
                     MGSVSSLLSSSGASDAGDGSLSLPSTGSVGVGAIAYSPSTFVLGTGDAATVTAAAAAV
                     PEPGTLSLLALAGVACLSRRARRST"
     gene            1047..2000
                     /locus_tag="VLI90_10245"
     CDS             1047..2000
                     /locus_tag="VLI90_10245"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSV14631.1"
                     /translation="MPSIDMPLEQMRQYRPSLYRESDFEPFWESTIAAAIKQPLNAEL
                     IPYDFPARGLESFAVRFDGFEGGRIAGWYLRPEKRGKFPGVCVYHGYSGRGPRPLDLL
                     TYAAQGMCVLSMDCRGQNGQSQDASNPPEGHAAGWMTQGIRDPRQYYYRYVYADAVRA
                     LELLARRDEVDEERLAITGGSQGGGISLAVSALAPRPPLLSLPDIPFLCDYRRAIAVA
                     CDGPYPEIPKFLKSFPHLYEQAIRTLSYCDCLNLAPRIKSRTVICNCLWDDVCPPSTI
                     YAVYNHISAEKQIEVYPFHKHETPHEHYETKFRLLMETLNP"
     gene            2123..3076
                     /locus_tag="VLI90_10250"
     CDS             2123..3076
                     /locus_tag="VLI90_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HSV14632.1"
                     /translation="MLSKTTTSLALALLLIATGCNRNDDNGVQGYVEGEYVYVASPMA
                     GALRSLSVDRGAEVKAGDPLFVLDDAPEKQARDEAERQVAQARATLADIKKGKRPSEI
                     ESLQAQLGRAKAALELSQLNLVRMEKLAPSGSVSKEELDRARSQREQDAHDVAALEAD
                     LTTAGLGQRPDQIAAAEAEVRAREAALAKADWNLSQKQQAAPLAGLVFDVLYRPGEWV
                     AAGRPVVALLPPGNIKVRAFVPETRVGAIHPGDSARVIIDGVATPATGKISYISPQAE
                     YTPPVIYSRETRAKLVFMVEIVFDDKTAATLHPGQPVDVQF"
     gene            3217..4158
                     /locus_tag="VLI90_10255"
     CDS             3217..4158
                     /locus_tag="VLI90_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV14633.1"
                     /translation="MNEPLAIDVQGMTKRFGALTAVDHIDLQVPRGQICGFLGPNGSG
                     KTTFIRMLCGLLRADDGSGQCLGYDVIRQSEAIKREVGYMTQRFSFYEDLSIVENLDF
                     VARMYGLRDRGRVVQQSLEQLGLSQRQHQLAAALSGGWKQRLALAACLLHDPKLLLLD
                     EPTAGVDPKARRDFWEQIHQLAGRGLTFLISTHYMDEAERCHRLAYIFQGKLLTQGTV
                     NEVVKKAGLTTWSVAGPDLLKLAEELRGKRGVDQTAAFGSVLHVSGSDAAALEQTLRD
                     HLTQSYQWRQIETGLEDVFIHLMDAHSNDDQKKEAAA"
     gene            4158..5303
                     /locus_tag="VLI90_10260"
     CDS             4158..5303
                     /locus_tag="VLI90_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV14634.1"
                     /translation="MGRAIATFSFRRFWAMVVKEFIQMRRDRLTFAMMVGIPLAQLVL
                     FGYAINSDPKHLPAAVVLADNGPQGRTVLYAIENSAYFDLVHRLRDEAEARDAMQRGT
                     VQFVINIPEDFTRKLWRGERPALLVEADATDPGATGNAVAALPGILNAALAHDLKGPL
                     ALLNAGDPPIDLRIHTLYNPEAITQYSIVPGLLGVVLTMTMTLITGLAITRERESGTM
                     ENLLSMPTRPLEVMLGKLVPYILVGYIQVGLILVAAQFLFHVPMVGSLTLLLTVSFAF
                     IAANLGVGITFSTIAKNQRQAMQMAMFFFLPSLLLSGFMFPFRGMPRWAQLLGETFPL
                     THFLRIVRGVMLKGNGAADVAQHLWPILLFLVVVLAIGVTRYRKTLD"
     gene            complement(5364..7943)
                     /locus_tag="VLI90_10265"
     CDS             complement(5364..7943)
                     /locus_tag="VLI90_10265"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HSV14635.1"
                     /translation="MSVLLSMVTVSAAAATNDDVAWPAITAEQKPWTRWWWLGSAVDK
                     PNLTRELQSFADAGIGGVEITPIYGAIGYEDRYIDFLSPKFMEMLRHVGTEGKRLGLG
                     VDMATGTGWPFGGPWIQPADADASVARDGDKFVSKPTRMKVKRAAPGDEGLVANPYSA
                     HAIDAYLAPFTKAFDGFPAGLIGGQFHDSFEYSGNWTDALPDRFKQMHGYALTDHIDE
                     LFGKGEADTVARVKSDYRETLAQLHMEYGEGWINWSHAHGFTTREQAHGAPANLLDLY
                     AAADVPETETFGFSDMPIPGFRAAGEGAHAQPQPMISRFASSAGHVAGRRLISSETFT
                     WLREHFNVALSQCKPVADQVFLAGINHIYFHGSAYSPDDVPWPGWLFYASTEFNPRNT
                     IWHDLPAMTAYIARCQSFLQAGAPDNDVLVYWPIYDLYHSADGMEKRFAMSPAKWAND
                     SPGGKTAQTLIDRGYAFDFVSDAQLIATKVADGALQTGGGSRYRVLLVPPCDHMPVET
                     LKQILDLAQAGATVIFQSHLPSDVPGLGDLENRRAKFKNEIARVQLSAAGIGGQMANV
                     GNGRVRVSDDPLKMLEVEHARRESIADTGVGFVRRRTDAGHDYFFANLTAKPIDGWIA
                     LGSPCESAVLMDPLTTRAGVAAVRKANNATEVRLQLRPGDSVLLRCFDARQANGPAWT
                     YLESTDAPLAINGTWQIDFLDGGPALPAAIKTDKLASWTTLGDDNAKRFAGTARYRIE
                     FDLPATQADDWQLDLGDVRESARVRINGQEAGTIWSLPFTLNVGPLLKPGARNVLELD
                     VTNLAANRIRDLDKQGGKWKAFHEINYVDIHYKKFDASNWPLVDSGLLGPVVLRPQRR
                     ANP"
     gene            complement(8077..8334)
                     /locus_tag="VLI90_10270"
     CDS             complement(8077..8334)
                     /locus_tag="VLI90_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14636.1"
                     /translation="MMPAESECGKKHHDLRKMPKNRRFFAKCVSHRQAAPLANLLVFY
                     ASAIVIDEPLRIFDSPGREAGDRMPNRAEFSFDPRPPGLGY"
BASE COUNT         1528 a         2590 c         2825 g         1520 t
ORIGIN      
        1 cgacgtgacg aacaactcgg cgttcgtcat caacgccaat accaccgcgt ccaacgtcag
       61 cggcgccggt ttcacgagcg tcaacgccac aaaattcttc aacaccacca acttcactca
      121 atccagcggc ttgatcaaca acggcaccac gcagatcaac ggcaacggca ccgtcggact
      181 gatcgccggc tccggcgcgc tgacgatcgg caacggcgcg accaccaaca cgctggcgct
      241 ggctcacaac agcggcggca gcacgcaggc cagcatcacg atcaacgccg gcgcgaagct
      301 cgacattaat aacaaccaca tgttcttcac gacgacgccg ctaacgacgg tccgcgggta
      361 tgtcatcagc ggcgcgatct tcagcgcgtt gtccgatacc acgcacgccg tgggctacgc
      421 gtcggtcgcc gggccgcagg tcgaggtgat gtggacgctg aagggcgacg cggacctcag
      481 cgggacagtc gatgttggtg acctcggtaa gctcgcgacc agctatggcg ccgtcacgac
      541 cggcgccacc tggttccaag gcgatttcac ctacgacggc accgttgacg tggccgacct
      601 cggcgcgctg gccaccaact acggcaccac gctggttggc ggggcgggcg atgcaatggg
      661 cagcgtcagt tccctacttt catcgtcggg tgcgtcggat gcaggcgatg gctcgctgag
      721 cctgcccagc accggcagcg tcggagttgg cgcgatcgcc tattcgccaa gcacgttcgt
      781 cctgggaacc ggcgacgccg cgacagtcac cgccgccgcc gccgcggttc cggaaccggg
      841 gacgttgtcg ctgcttgccc tggcgggcgt cgcgtgcctc tcgcggcggg cgcgcagatc
      901 cacctgatcc gccgccgctt gcggcttagc gtctgttcgc gaatggccaa aacacgctaa
      961 gccgcaagcg gcaattgaat catccccgct gacgggtatc ctccgcatcg gccagccgcc
     1021 gctggcgcga aggaggccat ccccccatgc catcgatcga catgccgctg gagcagatgc
     1081 ggcaataccg cccgtcgctt tatcgcgaat cggacttcga acccttttgg gaaagcacga
     1141 ttgccgcggc gatcaagcag ccgctgaacg cggagctcat cccgtacgac ttccccgcgc
     1201 gcgggctgga aagtttcgcc gtgcgattcg acggcttcga aggcggccgc atcgccggtt
     1261 ggtatctgcg gcccgagaag cgcggcaaat tccccggcgt gtgtgtctat cacggctaca
     1321 gcggtcgcgg cccgcggccg ctggatctgc tgacctacgc ggcccagggc atgtgcgtgc
     1381 tgagcatgga ctgccgcggg cagaacgggc aatcgcagga tgcgtcgaat ccgcccgaag
     1441 gccacgcggc ggggtggatg acgcagggca ttcgcgatcc tcgtcaatac tattatcgct
     1501 atgtatatgc cgatgcggtg cgcgcgctgg agctgctggc gcggcgagac gaggtggacg
     1561 aagagcgcct ggcgatcacc ggcggcagcc agggcggcgg catctctctg gcggtgtcgg
     1621 cgctggcgcc gcgtccgccg ctgttgtcgc tgccggacat tccgttcctg tgcgattatc
     1681 gccgggcgat cgccgtcgcc tgcgatgggc catatcctga aatcccgaaa tttctcaaaa
     1741 gcttcccgca cctgtacgag caggcgatcc gcacgctcag ttattgcgat tgcctgaacc
     1801 tcgccccgcg gatcaagagc cggacggtga tctgcaactg cctgtgggac gacgtgtgcc
     1861 cgccgagcac gatctacgcg gtgtacaacc acatctcggc cgagaagcag atcgaggtgt
     1921 acccgttcca caagcacgaa acgccgcacg agcattacga gacgaagttt cggctgttga
     1981 tggagacgtt gaatccataa gcgcccacga gccgcgcaag ccgatataaa taatagccgc
     2041 ggccggcagg cctggtcttt tctcctcgac cggcaagaag aaccgggcct gccggccccg
     2101 gctattaagg atgttcgcgt tggtgctgag caaaacaact acgagtctcg cgctagcgct
     2161 gttgctcatc gcgaccggtt gcaatcgcaa tgacgacaac ggcgtgcagg ggtatgtcga
     2221 aggggagtac gtctacgtcg cctcgccaat ggcgggggcg ctgcggtcgc tttcggtcga
     2281 tcgcggcgct gaggtgaaag ccggcgatcc gctgttcgtg ctggacgatg cgcccgaaaa
     2341 gcaggcgcgc gacgaggccg agcggcaggt cgcgcaagcg cgtgcgacgc ttgcggacat
     2401 caagaaaggc aagcggccaa gcgagatcga atcgctgcag gcgcaactgg gccgggcgaa
     2461 ggcggcgctg gaattgtcgc agctcaatct ggttcgcatg gagaagctcg cgcccagcgg
     2521 ttcggtgtcg aaagaagagt tggaccgcgc ccgctcgcaa cgggaacagg atgcgcacga
     2581 cgtcgcggcg ctggaggcgg acctgacgac cgcgggcctg ggccagcggc cggaccagat
     2641 cgcggcggcg gaggcggaag tgcgggcccg cgaagccgcg ctggcaaagg cggattggaa
     2701 tctatcgcag aagcagcagg ccgccccgtt agccgggctg gtgttcgacg tgttgtatcg
     2761 ccctggcgaa tgggtcgccg cgggccggcc ggtcgtcgca ctgctgccgc cggggaacat
     2821 caaagtgcgc gcgttcgtgc cggagacgcg cgttggggcg atccatccgg gcgacagtgc
     2881 gcgcgtaatc atcgacggcg tggcaacgcc ggcaaccggg aagatcagct acatctcgcc
     2941 gcaggcggag tacacgccgc cggtgatcta ttcgcgcgag acgcgcgcga agctggtgtt
     3001 catggtggag atcgtcttcg acgacaagac cgcggcgacg ctgcacccgg ggcagccggt
     3061 ggacgtgcag ttttgaacac gcgcgctggt gagattctgt attccgccgc gtagcggtcg
     3121 ctccgaagcg gcacgtgttt tctgcccgag cgccgaaaca cgtgcccctg cggggctacc
     3181 gctacgcagg ctgccgctac gcagtgaagt gacgccatga acgaaccgct tgccatcgac
     3241 gttcagggca tgaccaagcg cttcggcgcg cttaccgccg tcgatcacat cgacctgcag
     3301 gtgccccgcg ggcagatctg cggcttcctc ggtcccaacg gcagcggcaa gacgaccttc
     3361 atccgcatgc tgtgcggctt gctgcgcgcc gatgacggca gcgggcagtg tctgggctac
     3421 gacgtcatcc gccagagcga agcgatcaag cgcgaagtcg gttacatgac gcagcggttc
     3481 agcttttacg aagacctcag catcgtcgag aacctggatt tcgtcgcgcg gatgtacggg
     3541 ctgagagacc gaggtcgcgt cgtgcaacag agccttgaac agctcggcct cagccagcgc
     3601 caacatcaac tcgcggctgc gttgtcgggc ggatggaagc agcggctggc gctggcggct
     3661 tgcctgctgc atgatccgaa actgctgctg ctcgacgaac cgacggctgg cgtcgatccc
     3721 aaggcgcggc gggatttttg ggagcagatc caccagctgg cggggcgcgg gctcacgttt
     3781 ttgatcagca cgcattacat ggacgaagcc gagcgttgcc atcggctggc gtatattttt
     3841 cagggaaagc tgctcacgca ggggacggta aacgaagtcg tgaagaaggc gggattgacg
     3901 acgtggtcgg tcgcggggcc ggatttgctc aagctggcgg aagagttaag gggcaaacgc
     3961 ggcgtcgatc agaccgcggc gttcggcagt gtgctgcacg tcagcgggtc cgacgccgcc
     4021 gccttggagc aaacgcttcg cgatcatctt acgcagtcgt atcagtggcg gcagatcgaa
     4081 acgggactgg aggacgtgtt catccacctg atggacgctc acagcaatga cgaccagaag
     4141 aaggaagccg ccgcgtgatg ggccgcgcga tcgccacatt ttcgtttcgc cggttctggg
     4201 cgatggtcgt caaggaattc atccagatgc gccgcgaccg actgacgttc gcgatgatgg
     4261 tcggcattcc gctggcgcag ctggtcctgt tcggctacgc gatcaactcc gatcccaagc
     4321 acctgcccgc ggcggtggtg ctggccgaca acgggccgca ggggcggacc gtgctgtatg
     4381 cgatcgagaa tagcgcgtac ttcgatctgg ttcatcggct gcgcgatgaa gctgaagcgc
     4441 gcgatgcgat gcagcgtggg accgtgcagt tcgtcatcaa catccccgaa gatttcacgc
     4501 gaaaattatg gcgcggggaa cgtccggcgc tgctggttga agcggatgcc accgaccccg
     4561 gcgcgacggg caacgccgtc gcggcgctgc cgggcatttt gaacgcggcg ctggcgcacg
     4621 atctgaaagg tccgctggca ttgctgaatg caggcgatcc gccgatcgat cttcgcatcc
     4681 acacgctcta caaccccgaa gccatcacgc aatacagcat cgtccccgga ttgctcggcg
     4741 tcgtgttgac gatgacgatg acgctgatca ccgggctggc gatcacgcgc gagcgtgaaa
     4801 gcggcacgat ggagaatttg ctctcgatgc cgacccggcc gctggaggtg atgcttggca
     4861 agctcgtgcc gtacatcctg gtcggttaca tccaggtcgg gctgattctg gtcgccgccc
     4921 aattcctgtt tcacgtgccg atggtcggca gcctgacgct gctgctgacg gtatcgttcg
     4981 cgttcatcgc ggcgaacctc ggcgtcggca tcacgttctc gacgatcgcg aaaaaccagc
     5041 ggcaggcgat gcagatggcg atgtttttct tcctgccatc gctgctattg agcggcttca
     5101 tgttcccctt ccgcggaatg ccgcggtggg cccaactgct gggcgaaacc tttccgctga
     5161 cgcatttcct gcgcattgtc cgcggtgtga tgctgaaggg aaacggcgcg gccgacgtag
     5221 cgcagcacct gtggccgatc ctactgtttc tcgtggtcgt cctggcgatc ggcgtgacgc
     5281 gatatcgcaa gacgctggat tgaaagtagc acgggcgtct cgcccgtgtc ttgacaccgc
     5341 tcgacacggg cgagacgccc gtgctacgga ttagcccgcc gctgtggccg caggacgacc
     5401 gggccgagaa gaccggaatc caccagcggc caattcgacg cgtcgaactt cttgtaatga
     5461 atgtcgacgt agttgatctc gtggaacgcc ttccacttgc cgccttgctt gtccaggtcg
     5521 cggatgcggt tggccgcgag attggtcaca tccagctcaa gcacgttgcg cgcgccgggc
     5581 ttgagcagcg ggccgacgtt cagcgtgaac ggcaggctcc aaatcgtgcc ggcttcctgg
     5641 ccattgatgc ggacgcgcgc cgattcgcgc acgtcgccca ggtccagctg ccaatcgtcc
     5701 gcctgcgtcg cgggcagatc gaactcaatg cgatatcgcg ccgtgccggc gaagcgcttg
     5761 gcgttgtcgt cgccgagcgt ggtccagctg gcgagcttgt ccgtcttgat cgcggcgggc
     5821 agggcggggc cgccgtcgag gaagtcgatc tgccaagtgc cgttgatggc taggggggcg
     5881 tcggtggatt cgaggtaggt ccacgcgggg ccgttggctt gtcgggcgtc gaagcagcga
     5941 agcaagactg agtcgccggg gcgaagttgc aggcgaactt cggtcgcgtt gttggccttg
     6001 cgaacggcgg caacgccggc gcgggtcgtc agcggatcca tcagtacggc tgattcgcac
     6061 ggggaaccga gcgcgatcca accatcgatt ggcttggccg tcaggttcgc gaagaagtaa
     6121 tcgtgaccgg cgtcggtgcg ccggcggacg aaaccgacgc cggtgtcggc gatcgactcg
     6181 cgccgcgcgt gctcgacttc gagcatcttc agcgggtcat cactcacgcg cacgcggccg
     6241 ttgccgacgt tggccatctg cccgccgatg cccgctgccg atagttgcac gcgcgcgatc
     6301 tcgttcttga acttcgcccg gcggttctcc agatcgccca ggcccggcac gtcgctgggc
     6361 aggtgcgact ggaagatgac cgtggcgcca gcttgagcca aatcgagaat ctgtttcaac
     6421 gtttccaccg gcatgtgatc gcacggcggc acgagcagca cgcgatagcg cgacccgccg
     6481 cccgtttgca gcgcgccgtc ggcgaccttc gtcgcaatca gctgcgcatc ggaaacgaaa
     6541 tcaaacgcgt agccgcggtc gatcagcgtt tgggcggtct tcccacccgg cgaatcgttg
     6601 gcccatttcg cggggctcat cgcgaagcgt ttctccatgc cgtcggcact gtgatacagg
     6661 tcgtaaatcg gccaatacac cagcacgtcg ttgtcgggcg cgccggcttg aagaaacgat
     6721 tggcagcgcg cgatgtacgc ggtcatcgcc ggcaggtcgt gccagatcgt gttgcgcggg
     6781 ttgaattcgg tagaggcgta gaacaaccag ccgggccatg gcacatcgtc cggggaataa
     6841 gccgagccgt ggaagtagat gtggttgatg ccggcgagga agacctggtc ggccaccggt
     6901 ttgcactgcg agagcgcgac attgaaatgc tcgcgcagcc aggtgaacgt ctcggacgag
     6961 atcaaccgtc gcccggcgac gtgaccggcg gacgaggcga atcggctgat catcggttgc
     7021 ggttgcgcgt gcgcgccttc accggcagcg cggaagccgg ggatcggcat gtcggaaaat
     7081 ccgaacgtct cggtctccgg tacgtcggcg gcggcgtaaa gatcgagcag gttcgccggg
     7141 gcgccgtggg cctgctcgcg cgtcgtgaag ccgtgcgcat gcgaccagtt gatccagcct
     7201 tcgccgtatt ccatgtgcag ctgcgcgagc gtttcgcggt agtcgctctt cacgcgggcg
     7261 acggtgtcgg cttcgccttt gccgaacagc tcgtcgatgt gatcggtcag ggcgtagccg
     7321 tgcatctgct tgaatcgatc cggcaaggcg tcggtccagt tgcccgagta ttcgaaggag
     7381 tcgtggaact gcccgccgat caaaccggcg gggaagccgt caaaggcttt ggtgaacggc
     7441 gcgaggtacg cgtcgatcgc atgcgcggaa tacggattgg cgacgaggcc ctcatcgcca
     7501 ggggcggcgc gcttgacctt catgcgtgtg ggcttggaga cgaacttgtc accgtcgcgc
     7561 gcgacgctcg cgtcggcgtc ggcgggttgg atccacgggc caccgaaggg ccaaccggtg
     7621 ccggtggcca tgtccacgcc gaggcccagg cgtttgccct cggtgccgac gtggcgaagc
     7681 atctccatga atttcggcga gaggaaatcg atgtagcggt cttcgtaacc gatcgcgcca
     7741 tagatcggcg tgatctcgac cccgccgatg ccggcgtcgg cgaaggattg aagctcgcgc
     7801 gtgaggtttg gcttgtcgac ggcgctgccc agccaccacc agcgcgtcca gggtttttgt
     7861 tcggcggtga tcgcgggcca ggcaacatcg tcgttcgtag ctgccgctgc ggagactgtg
     7921 accatcgata acagaacgga cacgagaagg actgagttgt tcatttgagc gttcctcgag
     7981 cagcgcgcct gagatgaccg atcggacatt aaaccaatga agggatcgaa aaggaacgcg
     8041 ggcgttgatg gagattcgtt gcgcgcgggt gcgcgcttag tagcccaggc cgggaggccg
     8101 gggatcgaac gagaattcgg cgcgatttgg catccgatcc ccggcctccc ggcccgggct
     8161 atcaaagatg cgcagcggtt cgtcaatcac tatggcggat gcatagaaaa cgagcaggtt
     8221 cgcaagcggc gcggcctgac gatgagatac acacttcgcg aaaaatcggc ggtttttcgg
     8281 catttttcgc aaatcgtgat gcttctttcc gcactctgat tcggcaggca tcatcttcgc
     8341 tcttgtgttt cgcgtagccg ttatgtgacg gttgcgggtg acgcaaagca gtcactcatt
     8401 tcaatcatgg agcgcggttg gttcccacgg gcagtaccgc ccgcaggcgg tctgcgggcg
     8461 gta
//
LOCUS       DATIMH010000592         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2450365, whole genome shotgun sequence.
ACCESSION   DATIMH010000592 DATIMH010000000
VERSION     DATIMH010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450365"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1056)
                     /locus_tag="VLI90_10275"
     CDS             complement(100..1056)
                     /locus_tag="VLI90_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSV14637.1"
                     /translation="MAKVRKAVITAAGRGTRQYPASTAVQKEMFPLVDRDGLTKPVIQ
                     IIGEEAIDSGIEQICIITQPGEESLYRDYFKRLDDDMVKSFRGKDWAILESEKLGAFG
                     ERLSFAEQLTPEGFGHAVYQAKDFVGDEPFLLLLGDHIYISDIKDRCARQLIRVYEQY
                     MCDVVTGVQPTVERLLHLFGVLRGNPVDPARGIYKAELIIEKPSVETARENLITPMLP
                     AGNYLAHFGMHVFGPHIFDSLEYLIKNNIREKGEIQLTAAQEHLRQHTDKYWAVVTQG
                     QRYDTGIPYGLMETQLALALNGVHRTDICEAIARILAMQVKN"
     gene            complement(1377..2006)
                     /gene="pncA"
                     /locus_tag="VLI90_10280"
     CDS             complement(1377..2006)
                     /gene="pncA"
                     /locus_tag="VLI90_10280"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF008623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HSV14638.1"
                     /translation="MNALLIVDVQNDFVPGGALVVPDGDLVVPLINRVQPHFDLVVAT
                     QDWHPRDHASFAANHPGEKPGQTIDLNGLPQTLWPVHCVQGTSGADFVPGLDTAQIEA
                     IFRKGTDARIDSYSGFFDNGHRKRTGLAGYLNDRGVSDVYVCGLATDYCVKFTALDAR
                     QLGFNVHLIEDASRGVNLRAGDVDRAVDDMRNAGVSVSSSSHCVTGSGV"
BASE COUNT          358 a          653 c          656 g          454 t
ORIGIN      
        1 gttcggaggg tacccccacg acccggattc ttctcgaggt agagcaaagc atccgggtcg
       61 tcggagtacc tccgaacccg gcgtgtgtgg ggggcaaatc tagttcttca cctgcatcgc
      121 caggatgcgc gcgattgctt cgcagatatc cgtgcggtgc acgccgttca gcgccagcgc
      181 cagttgcgtt tccatcagcc catacggaat gcccgtgtcg tagcgctgcc cctgcgtgac
      241 gacggcccaa tacttatcgg tgtgctgccg cagatgttcc tgggcggccg tcagctgaat
      301 ctcgcccttc tcgcggatgt tgttcttgat caggtattca agcgaatcga aaatgtgcgg
      361 cccgaacacg tgcatgccga agtgcgccag gtaattcccc gcgggcagca tcggtgtgat
      421 caggttttcg cgcgccgttt ctacgctggg tttttcgatg atcagctccg ccttgtagat
      481 gccgcgcgcc ggatcgacgg ggtttccgcg cagcacgccg aacaggtgca gcaaccgctc
      541 gaccgtcggc tgcacgccgg tcacgacatc gcacatgtac tgctcgtaca cgcggatcag
      601 ctgccgcgcg cagcggtcct tgatgtcgct gatgtaaatg tgatcgccca gcagcagcag
      661 gaacggctcg tcgccgacaa agtccttcgc ctgatacacc gcgtgcccaa aaccctcggg
      721 cgttagttgc tcggcgaagc tcagccgctc accgaacgcg cccagctttt cgctttccag
      781 gatggcccaa tccttcccgc ggaaggattt gaccatgtcg tcatccagcc gcttgaagta
      841 atcgcggtac aaactctctt cgcccggctg ggtgatgatg cagatctgct cgatcccgct
      901 gtcgatcgcc tcttcgccga tgatctgaat gaccggcttg gtcagcccgt cgcgatcgac
      961 cagcggaaac atctccttct gcaccgcggt cgacgccgga tactgacgag tcccccgtcc
     1021 ggcggcggtg atgacggctt tgcgaacttt ggccatgggc ggttgtcctc ttcagttttc
     1081 gtgcggacga tattgcggtt ggtttaccaa tgttgcccgc gactcaagta tgcgcaccgc
     1141 ggtgtactgg taaactccac gccgtattta tttcaagggg tgtgatgatg tcggccgccc
     1201 gctcgcgccg cattttctcg agtatgtctg caccgtcatc cccgcggcga tgattggcgg
     1261 ccgatcggcg cgccgcgagc atcgcttccg tgatggccaa agacatctgc gtgtcatccg
     1321 tcgccgtatt cgaaaccggc gccgtaggcg tcacccacgc cgatttcgat caacattcaa
     1381 accccgcttc ccgttacgca atgcgatgat gacgagacgc ttacaccggc gtttcgcata
     1441 tcatcgactg ctcgatcgac atcgcccgcg cgcaggttca cgccgcggga ggcatcttcg
     1501 atgaggtgga cgttgaatcc cagttgccgc gcatcgagcg cggtgaactt cacgcaatag
     1561 tccgtcgcca ggccgcagac gtagacgtcg ctcacaccgc ggtcgttgag gtagccggcc
     1621 aggcccgttc gtttgcggtg gccgttgtcg aagaagccgc tgtagctgtc gatccgggcg
     1681 tcggtgcctt tacggaagat cgcttcgatt tgggcggtgt cgaggccggg gacgaagtcg
     1741 gcgccgctcg tgccttgcac gcagtgcacg ggccagaggg tctgcggcaa gccgttcagg
     1801 tcgatcgtct ggccgggctt ttcgccggga tgattggcgg cgaagctggc gtggtcgcgc
     1861 gggtgccagt cttgcgtggc gacgaccaga tcgaagtgcg gctggacgcg gttgatgagg
     1921 ggcacgacga gatcaccatc cggcaccacc agcgcgccgc cggggacgaa atcgttttgc
     1981 acgtcgacga tgagaagtgc gttcactgga tttgctccgc gtcgggcagg ccgcgcgcct
     2041 ttaggatcag cttcgttttc agctcgtgca agtttcgctc cagtccgacc gggtacaggt
     2101 gcggattgat gaagcgctgg g
//
LOCUS       DATIMH010000593         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5344448, whole genome shotgun sequence.
ACCESSION   DATIMH010000593 DATIMH010000000
VERSION     DATIMH010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5344448"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VLI90_10285"
     CDS             <1..926
                     /locus_tag="VLI90_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14639.1"
                     /translation="VYNTSMSRPDAALALAELCGFQGKREARIGSVCVTGSGLNAAAF
                     CDIVMRFYTLGPPRNANQVLPVGLAAVEPLPPDPPMVRAAVAGSYARSVKKLSDTSLA
                     EAVLRNGVIFNAEAAVILSAPATCLAKSLDLLGVKQLYKERVKRLVIVDAGERQDAAS
                     LRKVIAEWPTPIFYCGREVGEALPFPGERVATDFAWAPAHPVVDAYRAYKPGAFDAPS
                     YDVAAAHFAVHPDSGFFQLSDAGSIAVEDDGRMKFTPGNGTVKALKVDPAQKSKIIER
                     FVELASAKPVAPPQRLRPPSDTKAPPPAAKK"
     gene            967..1293
                     /locus_tag="VLI90_10290"
     CDS             967..1293
                     /locus_tag="VLI90_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HSV14640.1"
                     /translation="MPQDRTIWERIAESEEFKDLMAAKARFIVPATMFFVVYYFALPV
                     LVGYAPELMSRRVIGVVNVAYLFALSQFFMAWILAWLYVRAAGRFDRMAKRILENIKP
                     EPKGRD"
     gene            1321..>2634
                     /locus_tag="VLI90_10295"
     CDS             1321..>2634
                     /locus_tag="VLI90_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HSV14641.1"
                     /translation="MFIAITLGITMWAARRSSGASAYFAAGRQIKGWQNGIAVAGDYM
                     SAASFLGIAGIIAFQGYDGFMYSVGWLVAYLTVLLIVAEPLRNAGKYTMADVLAYRLR
                     PRPVRAMAALSTLTVSTFYMIAQMVGAGTLVALLLRGSGISFNLAVAIVGVLMVAYVV
                     FGGMLATTWVQIVKAILLIAGTILLSVLVLAHFHFSFGEFFDAIAHVKDGGRVVNYLE
                     PGLRYKPPYGALDLISLGLALIFGTAGLPHILVRFYTVPDAKTARISVVWAMVIIGGF
                     YIMTTFLGFGAATIVGKGFIAQNGGINMSAPLLAQRLGGDVFFAFISAVAFATILAVV
                     AGLTISASTSFAHDLYSNVFHYGKPRTQEQEVRVARITAFVVGAISIAVAILLGPTFN
                     VAFLVGLAFAVAASANLPVILLSIFWKRFTTEGAVWGLATGLISSL"
BASE COUNT          429 a          764 c          881 g          560 t
ORIGIN      
        1 ttgtctacaa cacgtcgatg agccggcctg acgccgcgct ggcgctggcg gaattgtgcg
       61 gctttcaagg caagcgcgag gcgcgcatcg ggtcggtgtg cgtcacgggg tcgggactga
      121 atgccgcggc cttctgcgac atcgtcatgc ggttctacac tctcggaccg ccgcggaatg
      181 ccaaccaggt gctgccggtc ggactcgcgg ctgttgagcc actgccgccg gatccgccga
      241 tggtgcgggc ggctgtggcc gggtcgtacg cccgttccgt caagaagctg agcgacactt
      301 cgctggcgga ggcggtgctg cggaatggcg tgatcttcaa tgccgaagca gcggtgatcc
      361 tgagcgcgcc cgcgacgtgc ctggcgaaat cgctcgatct gctgggtgtc aagcagttgt
      421 acaaggaacg ggtgaagcgg ctcgtgattg tcgatgccgg cgagcggcag gacgccgcgt
      481 cgctgcgtaa ggtgatcgcc gagtggccga ctccgatttt ctactgcggt agagaagtcg
      541 gcgaggcgtt gccgtttccc ggcgagcgcg tggcaacgga cttcgcgtgg gctccggcgc
      601 atccggtggt cgatgcgtat cgcgcgtaca aaccgggagc gttcgacgcg ccgtcgtatg
      661 atgtcgcggc ggcacacttt gccgtgcatc cggattcggg gttcttccag ttgtcggatg
      721 cggggtccat cgccgttgag gatgatggcc ggatgaagtt cacgcccggc aacggcactg
      781 tgaaggcgct caaggtagat ccggcacaga aaagcaagat catcgagaga tttgtcgaac
      841 tcgccagcgc caaaccggta gctcctcccc agcgactcag gccgccatcg gatacgaagg
      901 cgcctcctcc cgccgccaaa aagtagcgca gggctggcgt atgatggaca atcgccgagg
      961 agagacatgc cgcaggatcg aactatctgg gagcgtatcg cggagagtga ggaattcaag
     1021 gacctgatgg cagccaaggc ccgattcatc gtgccggcca caatgttctt cgtcgtgtac
     1081 tactttgctc tgcctgtcct ggtgggttat gcgcctgaac tgatgagccg ccgcgtgatc
     1141 ggggtggtga acgtcgctta cctgttcgcg ctctcgcagt tcttcatggc gtggatcctc
     1201 gcatggctat atgtgcgcgc agccggcagg ttcgaccgga tggcgaagcg cattctggag
     1261 aacatcaaac cggaaccgaa ggggcgcgac taaatgtcga ctcctctgtt gatgtttctc
     1321 gtgttcatcg cgatcacgct cggcattacg atgtgggcgg cgcggcgatc gagcggcgca
     1381 agcgcgtatt tcgcggctgg acggcaaatc aagggctggc agaacggcat cgccgtcgcc
     1441 ggcgattaca tgagtgccgc gtcgtttctc gggattgcgg ggattattgc tttccagggg
     1501 tacgacggtt tcatgtactc ggtggggtgg ctggtcgcgt atttgacggt gctgctgatt
     1561 gtcgccgaac cgctgcggaa tgccggcaag tacaccatgg cggatgttct cgcataccgg
     1621 ctgcggccgc gccccgtgcg cgccatggca gcgcttagca ccctgacggt cagcacgttt
     1681 tacatgattg cgcagatggt cggcgccggg acgctcgtgg cgctgctgct gcgcgggtcc
     1741 gggatctcgt tcaatctggc ggtcgcgatt gtcggcgtgt tgatggtggc ctacgtggtt
     1801 ttcggcggca tgctcgcgac gacgtgggtg cagatcgtca aggccattct gctgatcgcc
     1861 ggcacgatcc tgctcagcgt gcttgtgctg gcgcatttcc acttcagctt cggcgagttc
     1921 ttcgacgcca tcgcgcacgt gaaggacggc gggcgggtcg tcaactatct ggagccgggg
     1981 ctgcgctaca agccaccata tggagcgctc gatttgatct cgctgggact cgcgctgatc
     2041 ttcgggactg ccggtctgcc acacattctc gtccgcttct acaccgtccc cgacgcaaag
     2101 acggcgcgca tcagtgtcgt gtgggcgatg gtgattatcg gcggcttcta catcatgacc
     2161 acattcctgg gcttcggcgc ggcgacgatt gtcggcaagg gcttcatcgc tcagaacggc
     2221 ggcatcaata tgagcgcgcc tctgttggcg caacgccttg gtggcgacgt cttcttcgcc
     2281 ttcatctcag cggtggcgtt tgcgacgatt ctggctgtcg tcgccggatt gaccatcagc
     2341 gcgtcgacgt ccttcgcgca cgacctgtat agcaatgtgt tccactacgg aaagccgcgc
     2401 acgcaggagc aggaggtcag agtggcgcgc attacggcgt tcgtggtcgg cgccatctcg
     2461 atcgccgtgg cgatcctcct cggcccgacg ttcaacgtcg cgttcctggt gggactggcg
     2521 ttcgcggtcg cggcgtcggc gaatttgccc gttatcctgc tgtcgatttt ctggaaacgc
     2581 ttcactaccg aaggcgcagt ctggggactg gcaacgggcc ttatcagttc gctc
//
LOCUS       DATIMH010000594         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5055636, whole genome shotgun sequence.
ACCESSION   DATIMH010000594 DATIMH010000000
VERSION     DATIMH010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5055636"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2121
                     /locus_tag="VLI90_10300"
     CDS             <1..2121
                     /locus_tag="VLI90_10300"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HSV14642.1"
                     /translation="RGSYGQRYDNGQRHDGIKTQKLPYPPLTKGPDTHWDAPGMQRIK
                     IPFGGMTPEQMIVLAELAEEYSDGICHITTRQDIQLHFVHIDTVPDIFRRLAAVGITT
                     REACGNSVRNVTACPLAGVCRTESFDVTPYAKAAAFYLLGHRDTQDFGRKFKIAFSGC
                     EHEACALVSIHDLGGIAKVRTGPDGKQQRGFAIYVGGGLGAVPHQAKLFEEFVAEEDI
                     LPLARAIGRVFARLGEKKNRNKARLKFVVQKLGVDEFRRLVREEVATMPEDPSWRKYF
                     DEIPRYDEKPAWQGVQLNGARRPEGFDAWAATNVYEQRQSGYAVATVTCPLGDLTSDQ
                     MRALADIGARYAGGNVRTTVEQNIVLRWVPQDKLIDLYNELKALDLATPGAGTIVDVA
                     ACPGTDTCKLGIASSRGLAGELRDRLASKSASMPQAIKNLRIKISGCFNSCGQHHVAD
                     IGFYGNSRNVGNYTVPHFQVMLGGQWMHNGGSYALAMGSVPSKAIPDLVAALTDRYVN
                     ERQGEETFQAWCQRIGKKELKSIVEKFAPVPAHKDDASFYTDWGDPREFTIGDLGTGE
                     CAGEVISVSQFGFTLAESEAFEAQLSLDAGQYKVADEKAYQAMLTAAKTLVQNQWLDV
                     PDEANVIVDEFRNRYVETKLFWDTYHGDQFSRYLFQRHEGADGRFNADTAHKIVEEAN
                     LFIDAAHKCELKWRQQNAGGNAAA"
     gene            2310..2912
                     /locus_tag="VLI90_10305"
     CDS             2310..2912
                     /locus_tag="VLI90_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14643.1"
                     /translation="MDLDKINVKLFTKTDTFAPAEFVPIFHHWIQEQSLPGHLLIDVA
                     DYAHVPAGPGTLVVASEANIHMDRTENRLGLLYVRKQPIVGAATLHDRIRAVIAEALH
                     AAAKMQHEPECEGRLDFRTDEISIRFNDRLLAPNTPETLAALKPAVEAVAKKLFANSP
                     VTVELHDPSPLVLLDLRVKTNGSTTIEQLLERAGAQSLSN"
BASE COUNT          643 a          964 c          955 g          471 t
ORIGIN      
        1 cgcggatcgt acggccagcg ctatgacaat ggccagcgcc atgacggcat caagacgcag
       61 aagctgccct acccgccgct gaccaaaggt cccgacaccc attgggacgc gccgggcatg
      121 cagcggatca agatcccctt cgggggaatg acgcccgagc agatgatcgt gctggccgag
      181 ctggcggagg aatactccga cggcatctgc cacatcacga cccgccagga catccagctg
      241 cacttcgtcc acatcgacac cgtgccggat atttttcgaa ggctcgccgc cgtcggcatc
      301 acgacgcgcg aggcctgcgg caacagcgtg cgcaacgtga ccgcctgccc gctggccggt
      361 gtctgccgga ccgagtcgtt cgacgtcacg ccgtatgcca aggccgccgc gttttatctg
      421 ctcggccacc gcgacacgca ggactttggc cgcaagttca agatcgcctt cagcggttgc
      481 gagcacgaag cgtgcgcgct ggtgtcgatc cacgacctgg gcggcatcgc gaaggtgcgc
      541 acgggtcccg acggcaagca acagcgcggc ttcgccatct acgtcggtgg cgggctgggc
      601 gcggtgccgc atcaggcaaa gctgttcgaa gaattcgtgg cagaagaaga catcctgccg
      661 ttggcccgcg cgatcggccg cgtgttcgcc cggctgggtg agaagaagaa tcgcaacaaa
      721 gctcggctga agttcgtcgt gcagaagctg ggcgtcgacg aattccgccg gctcgtgcgg
      781 gaagaagtcg cgacgatgcc ggaagatcca tcttggcgga aatatttcga cgagatcccc
      841 cgctacgacg aaaaacccgc ctggcaaggc gtgcagctga acggcgcccg ccggcccgag
      901 ggtttcgatg cctgggccgc gacaaacgtg tacgagcagc ggcaaagcgg ttacgccgtc
      961 gcgaccgtca cctgcccgct gggtgatctg accagtgacc agatgcgcgc cttggcggac
     1021 atcggcgcgc gttacgccgg tggaaacgtg cggacgacgg tcgaacaaaa catcgtgctg
     1081 cgctgggtgc cgcaggacaa gctcatcgac ctgtacaacg aactgaaggc gctcgatcta
     1141 gcgacgcccg gcgcggggac gatcgtggac gtcgccgcct gccccggcac tgatacgtgc
     1201 aagctgggca tcgcctcatc ccgcgggctg gctggtgaac ttcgcgatcg gctcgcatca
     1261 aaaagtgcat cgatgccaca ggcaatcaaa aacctgcgca tcaagatctc cggttgcttc
     1321 aacagctgcg gccagcatca cgtggccgac atcgggttct acggcaacag ccgaaacgtc
     1381 ggcaactaca ccgtcccgca cttccaggtg atgctgggcg ggcaatggat gcacaacggc
     1441 ggcagctatg cgctggcgat gggttcggtg ccgagcaagg ccattcccga tctcgtcgcg
     1501 gcgctgactg atcgctacgt caatgaacgc cagggcgaag agacgttcca ggcgtggtgc
     1561 cagcgcatcg gtaagaagga gctgaagtcg atcgtcgaaa agttcgcgcc ggtgccggcg
     1621 cacaaggacg atgccagctt ctacaccgat tggggcgacc cgcgcgagtt cacgatcggc
     1681 gacctcggca ccggcgagtg cgcgggtgaa gtgatcagcg tcagccaatt cggcttcacg
     1741 ctggccgaaa gcgaagcctt cgaagcgcag ctgtcgctcg acgcgggcca gtacaaggtg
     1801 gcggacgaga aggcgtatca ggcgatgctg acggcagcca agacgctggt gcagaaccag
     1861 tggctcgacg tgcccgacga ggcgaacgtg atcgtcgacg agttccgcaa ccgctacgtc
     1921 gagaccaagc tgttctggga cacgtaccac ggcgaccagt tcagccgata tctgttccag
     1981 cgccacgaag gcgccgatgg ccgattcaac gccgacacag cccacaagat cgttgaagag
     2041 gcgaacctct tcatcgacgc cgcccacaag tgcgaactga aatggcggca acaaaatgcc
     2101 ggaggcaacg ccgccgccta atctgccggt gccgccgcga cgtaaaacgc ccccatttag
     2161 cccggcgtga acacacgccg ggtttacgtg ggacatcaag aacctcagat cgagaaatcg
     2221 aacggccaag acttctcaac aaaacccggc gtgtgttcac gccgggctaa atggggtgtg
     2281 agacgaatcg atcggccttt agaaaatcca tggacctcga caaaattaac gtcaagctct
     2341 tcaccaagac cgacaccttc gcgccggcgg agttcgtgcc gatctttcac cactggatcc
     2401 aggagcagtc gctgcccggg cacctgctga tcgacgtggc ggactacgcc cacgtccccg
     2461 ccggtcccgg cacgctggtc gtcgcgtccg aagcgaacat ccacatggac cgcaccgaga
     2521 atcggcttgg cctgctgtac gtccggaagc agccgatcgt cggggcagcg acgctgcacg
     2581 atcgcattcg cgcggtgatc gccgaagcgc tgcacgccgc cgcgaagatg cagcatgagc
     2641 cggaatgcga aggccggctc gacttccgca cggacgagat ttcgattcgg ttcaacgatc
     2701 gcctcctcgc gccgaataca ccggaaacac tcgccgcgct gaagccggcg gttgaagcgg
     2761 tggcgaagaa gctgttcgcg aattcgccgg taacggtcga gctgcacgac ccatcgccgc
     2821 tggtgctgct ggacctgcgc gtgaagacga acggatcgac gacgatcgaa caactcctgg
     2881 aacgagcggg cgcgcaatcg ctctcgaact gaccccacgc cgggttcgga gcgtactcgc
     2941 gacgaagcga gattctgcgg cgcgtagaga agaagaaccc gggtcgtcgg aatacctccg
     3001 aacccggcgt ggcatcactt cgatgacaac gcc
//
LOCUS       DATIMH010000595         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3032678, whole genome shotgun sequence.
ACCESSION   DATIMH010000595 DATIMH010000000
VERSION     DATIMH010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3032678"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1488)
                     /locus_tag="VLI90_10310"
     CDS             complement(22..1488)
                     /locus_tag="VLI90_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSV14644.1"
                     /translation="MRSCTVLHICIGLIIASATPHAQALIESARGNDPVADMNWPTGA
                     VDLANFKTRVGWWEGPPFGGGERCFQYRGDTAACTEAPAAFARVKAPQLLLIVHEGPG
                     NGQFLKDSQDPNADTSLDWELTLWTPENFHRLFNNPKSAFDSVVPEFRGELPPPKLDV
                     YPSNGRIDWSRVAVPANVTVRDERAIASGYKVEDGSVVRGAVYDMLTSKPIAGVDVTI
                     ERRITSSTTQPTTSPAGEYESAAAGTSDADGHYQITHIAPGGYRIVLRHAGHAARMLG
                     WFSFGGHTLKTFTTQMVKAVDATLTVSQASGERLTGVEVRCENTIGLDGRGYPLPEEP
                     AVVSDMSGSVTFSGLPRGYAQFFASAKGFHQLEMMTLRELPVERIQLVMAATGSLRGH
                     VKLAPGVAATNVHVQVRPEGEVIGKWSGSMQVKPDGTFAFDDVPPGRYFASTDPMFAF
                     TGKDANAATVTVGAGKVSEVEVSPPPAPVKPPRRAKGK"
BASE COUNT          262 a          549 c          464 g          282 t
ORIGIN      
        1 acgctaagcc gcaagcggct gctactttcc cttcgcgcgc cgcggtggtt tcaccggcgc
       61 cggtggcgga ctcacctcaa cctccgagac tttccccgcc cccaccgtca ccgtcgccgc
      121 gttcgcatct ttgccggtga acgcgaacat cggatccgtg ctggcgaaat accgccccgg
      181 cgggacatcg tcgaacgcga acgtgccgtc cggcttcacc tgcatcgaac cgctccattt
      241 gccgatcacc tcgccctcgg gccgcacttg gacgtgcacg tttgtcgcgg cgacgcccgg
      301 cgccagcttc acgtgaccac gcaggctgcc ggtggcagcc atcaccaatt gaatccgctc
      361 caccggcagc tcgcgcagcg tcatcatctc cagttggtga aaacccttag ccgacgcgaa
      421 gaactgcgcg taaccgcgcg gcaggccgct gaacgtgacc gagccgctca tgtcgctcac
      481 gaccgccggc tcttccggca acgggtaccc gcggccatcg aggccgatcg tattctcgca
      541 gcgcacctcc acgcccgtca gccgttcgcc acttgcctgg ctgaccgtca acgtcgcgtc
      601 cacggctttg accatctgcg tcgtgaacgt cttgagcgtg tgcccgccga acgagaacca
      661 gccgagcatt cgcgccgcgt gccccgcgtg gcgcaagacg attcgataac cgcccggagc
      721 aatgtgcgtg atctggtaat gaccatcagc atcgctcgtc cccgcggcgg cggattcata
      781 ttcgccagcc ggcgatgtcg tgggttgcgt agtggacgag gtgattcgcc gctcgatcgt
      841 cacatcgacg ccggcgatcg gcttgctcgt gagcatgtcg tacacagcgc cgcggaccac
      901 cgaaccgtct tcgactttgt aaccactcgc tatcgcacgc tcgtcgcgca ccgttacgtt
      961 cgccggcacc gccacgcgcg accaatcgat ccggccatta ctgggataca catccagctt
     1021 cggcggcggc agctcgccgc ggaactcggg gactaccgaa tcgaacgcgc tcttcggatt
     1081 gttgaacaag cggtgaaaat tctccggcgt ccagagcgtc aactcccaat ccaggctcgt
     1141 gtcggcgttc ggatcctggc tgtctttgag aaactgcccg ttgcctggtc cctcatgcac
     1201 gatcagcagc agctgcgggg ccttaacgcg ggcgaacgcc gccggcgcct cggtgcacgc
     1261 cgcggtgtcg ccacgatatt ggaagcagcg ttcgccgccg ccgaagggtg ggccttccca
     1321 ccagccaacg cgggtcttga agttggccag gtccaccgcg ccggtgggcc agttcatgtc
     1381 ggcgaccgga tcgttcccgc gggcggattc aatcaatgcc tgcgcatgcg gcgtcgccga
     1441 ggcgatgatg agaccgatgc agatgtgaag gacggtacag ctgcgcatga cttctcccgg
     1501 gatacagacg cggcggcgat gctcaagttc ccttccccgt ccacgccggg ttcggag
//
LOCUS       DATIMH010000596         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2452024, whole genome shotgun sequence.
ACCESSION   DATIMH010000596 DATIMH010000000
VERSION     DATIMH010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452024"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /gene="rfbC"
                     /locus_tag="VLI90_10315"
     CDS             <1..447
                     /gene="rfbC"
                     /locus_tag="VLI90_10315"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HSV14645.1"
                     /translation="ADAGIDVEFVQDNHSLSAEKGVVRGMHWQLPPMAQDKLVRVVRG
                     AILDVAVDIRRGSPTFGQQVKVLLSAENWKQIFVPVGFAHGFVTLEPNTEVIYKVTAG
                     YSPQHERGFRWNDPNVGIEWGIGEDAATLSGRDRAHPLLKDVKDLF"
     gene            488..1366
                     /gene="rfbA"
                     /locus_tag="VLI90_10320"
     CDS             488..1366
                     /gene="rfbA"
                     /locus_tag="VLI90_10320"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HSV14646.1"
                     /translation="MKGILLAGGAGTRLYPLTRCISKQLLPVYDKPTIYYPLSALMLA
                     GIREILIISTPRDLPRIRDLFGDGSQFGLKLSYKEQQQPNGIAQAFVLGAEFVGNSPV
                     CLILGDNIFYGHELSDMLEDSAKLTSGARVFAYQVKDPERYGVVAFDKSFKASSIEEK
                     PKQPRSNWAVTGLYFYDASVVDIAKNLKPSARGEYEITDVNRAYLERGQLQVQCMGRG
                     IAWLDTGTYDSLLSSSVFVQTLEERQGLKVACIEEIAFAKKFIDANQLKKLADQAGKS
                     EYGQYLLRVLKDAAVQ"
     gene            1515..1901
                     /locus_tag="VLI90_10325"
     CDS             1515..1901
                     /locus_tag="VLI90_10325"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSV14647.1"
                     /translation="MIIAYHHTQITAPKGAEADVRRFYRDVLGLKETPLPEALRDRNL
                     IWFEVGASTLHVGFEDGVNRAATNAHLAYEVDDIAAFRRRVKDAGYELIEQPKIAGYD
                     RFHTHDPFGNRLEIIGRNDVARATSP"
     gene            1952..>2717
                     /locus_tag="VLI90_10330"
     CDS             1952..>2717
                     /locus_tag="VLI90_10330"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HSV14648.1"
                     /translation="MRTILVTGAAGFIGSNFVRMLITRGEPVKLVALDKLTYAGNLAN
                     LQDLLKDPRLVFIRGDICDKPLLTELWDEHGIREVVHFAAESHVDRSILDSGPFIQTN
                     VVGTQVLLDVARAKGVEKYLQVSTDEVYGSLPEDKPEIKFTEQTPLEPNSPYSASKAA
                     ADCLVRSYFHTFHMPVLTTRCSNNYGPYHFPEKMIPLFVTNLMEGKKVPLYGDGMNVR
                     DWLYVEDHCDGIWTVLNKGKFGDVYNIGGHNEIPNRR"
BASE COUNT          572 a          863 c          824 g          458 t
ORIGIN      
        1 gccgacgcgg ggatcgacgt cgagttcgtg caggacaacc attcgttgtc ggccgaaaaa
       61 ggcgtcgtcc gcggcatgca ctggcagctg ccgccgatgg cgcaggacaa gctggtgcgc
      121 gtcgtccgcg gggcgatctt agatgtcgcg gtcgatatcc gccggggctc gccgacgttc
      181 ggccagcagg tgaaggtgct gctgtcggcc gagaattgga agcagatctt cgtccccgtc
      241 ggcttcgccc acggcttcgt cacgctcgag ccgaacacgg aggtcatcta caaggtgacc
      301 gccggctatt cgccgcagca cgaacgcggc tttcgctgga acgatccgaa cgtcggcatc
      361 gagtggggca tcggtgagga tgcggcgaca ctctcgggcc gcgaccgcgc gcacccgctc
      421 ctgaaggacg tgaaggacct gttctgattc cgccgccgct gcgccatccg agataaggac
      481 cagcattatg aagggcattc tcctcgccgg cggggccggc actcggcttt atccgctgac
      541 gcgctgcatc agcaagcagc tgctgccggt ctacgacaag ccgacgattt actatccgct
      601 gtcggcgctg atgctcgcgg ggattcgcga gatcctgatc atcagcacgc cgcgcgacct
      661 gccgcgcatc cgggatttgt tcggcgatgg ttcgcagttc ggtctgaagc tcagctacaa
      721 ggagcagcaa cagccgaacg gcatcgcgca ggcgtttgtg ctgggcgcgg agttcgtcgg
      781 caactccccg gtctgcctga tcctcggcga caacattttc tacggccacg agctgagcga
      841 catgctcgaa gactccgcca agctaaccag cggcgcccgc gtcttcgcgt atcaagtgaa
      901 agaccctgag cgctacggcg tcgtcgcgtt cgacaagagc ttcaaggcgt cgagcatcga
      961 ggaaaaaccc aagcagccca ggagcaactg ggccgtcacg ggactgtact tctacgatgc
     1021 cagcgtcgtc gacatcgcga agaacctgaa gccgtcggct cgcggcgaat acgagatcac
     1081 cgacgtcaac cgcgcctatt tggaacgcgg gcaattgcag gtccagtgca tgggccgcgg
     1141 catcgcctgg ctcgacacgg gaacctacga ttcgctgctg agcagctcgg tcttcgtgca
     1201 aacgctggaa gagcggcagg gcctaaaggt cgcgtgcatc gaagagatcg cgttcgcgaa
     1261 gaagtttatc gatgcgaatc agctgaagaa gctcgccgac caagcgggga agagcgagta
     1321 tgggcagtat ctcctgcgcg tcttgaagga cgcagcggtg cagtaagcga ggatctgccg
     1381 agagacgccc tgatagccgc aggcgacagc cagcgggggc gctcgacgta gaacccggtg
     1441 cgaattcgcg aaaaccccgc tggctatcgc ctgcggctat tacagtaaat gttcatcgcg
     1501 cagaacgagt acgaatgatc atcgcgtacc accatactca gatcaccgcc ccaaagggcg
     1561 ccgaagccga cgtccgccgc ttctaccgcg acgtcctggg cctgaaggaa accccgttgc
     1621 ccgaggcgct ccgcgaccgc aacctgatct ggttcgaggt cggcgccagc accctgcacg
     1681 ttggcttcga agacggcgtc aaccgcgcgg caaccaacgc gcacctggcg tatgaagtgg
     1741 acgacatcgc tgccttccgc cgccgggtga aagacgcggg atatgaattg atcgaacagc
     1801 cgaagatcgc gggctacgac cgcttccaca cgcacgaccc gttcggcaat cgcctggaaa
     1861 tcatcggccg aaatgacgtg gcacgggcta ccagcccgtg attttaaatc attaatttta
     1921 aacacgggct gatagcccgt gctactgaaa catgcgcacc atcctcgtca ccggcgccgc
     1981 cgggttcatc gggtctaact tcgtccgtat gctgatcacg cgcggcgagc ccgtgaagct
     2041 cgtcgcgctc gacaagctca cctacgcagg caacctggcc aacctgcagg acctgctcaa
     2101 agacccgcgg ctggtcttca tccgcggcga catctgcgac aagccgctgc taaccgagct
     2161 ttgggacgag cacggcattc gcgaggtcgt gcacttcgcc gccgagagcc acgtcgatcg
     2221 cagcatcctc gactctggcc cgttcatcca gaccaacgtc gtcggcacgc aggtgctgct
     2281 cgacgtcgcg cgggcgaagg gcgtcgaaaa atatcttcag gtcagcacgg acgaagtgta
     2341 cggctcactg ccggaggaca agcccgagat caagttcacc gagcagacgc cgctcgaacc
     2401 caacagccca tacagcgcga gcaaggccgc cgcggattgc ctggtgcgat cgtacttcca
     2461 cacgttccac atgccggtgc tgacgacccg ctgcagcaac aactacgggc cgtaccattt
     2521 tcccgagaag atgatcccgc tgttcgtcac caacctgatg gaaggcaaaa aagttccgct
     2581 ctacggcgac ggcatgaacg tgcgcgattg gctctacgtg gaagaccact gcgacggcat
     2641 ctggacggtg ctgaacaaag gcaaattcgg cgacgtctac aacatcggcg ggcacaacga
     2701 aatccccaac cgccgga
//
LOCUS       DATIMH010000597         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1011831, whole genome shotgun sequence.
ACCESSION   DATIMH010000597 DATIMH010000000
VERSION     DATIMH010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011831"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VLI90_10335"
     CDS             <1..458
                     /locus_tag="VLI90_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSV14649.1"
                     /translation="MRDAFRKLTADETAKRLDAVRDYAIALPSATDRTGTIGFCWGGG
                     ASFAYAAHQPKLNAAVVFYGTPPAKETLANIACPVYGFYGKDDNRVTSTVEPTKQAMS
                     ALNKPYEPHIYEGAGHGFMRQQDGRDGANQKAADAAWPQAIGFLKNHLK"
     gene            471..1250
                     /locus_tag="VLI90_10340"
     CDS             471..1250
                     /locus_tag="VLI90_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14650.1"
                     /translation="MMDLNALRQRLDAERRTLLRRGEMVEVLRHLTRVQRDTQHEISF
                     ASLPSAGADAIIAEQIEHYRNLNTPVEWKVYAHDQPHDLRHRLIANGFSRGPEEAVLV
                     LDLRNPPEWISAAAADAPVVRVTTDDQVKMFRDAAAEIFAKDYDWTAGQLRYGIAARS
                     TEHLGYIAIEGNHAISIGRLYTHHDRHFGGLYGGGTRATHRGRGLYRAVVAARARDAI
                     ELGARYLIVDALPTSQPILRRLGFIELSKTWPCTWHPGSER"
     gene            1527..2351
                     /locus_tag="VLI90_10345"
     CDS             1527..2351
                     /locus_tag="VLI90_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSV14651.1"
                     /translation="MKPNDDRSAWVQRVLHRVRALQRSRAARDASHHFWIEGVRQFVQ
                     AVDAGLTFETVVISPILLQSDLADMLARRLQTQRVPRARLTPEQFRSISTTVRASGIG
                     AIVRQHYSPLSSLDARAGLCWLVIERLRSPGNLGTILRPAEAVGAAGVIFLTSACDPF
                     DPAAVRASMGGIFHLRLAVCSPRQYRQWAKRHGVTTAGLAPRVRQLWTQLPAARPIAI
                     LLGEERRGLTIETAGLCDLTVQLPMSGRADSLNVAIAAGVMLYELVRRQHLPGLVV"
BASE COUNT          457 a          807 c          777 g          410 t
ORIGIN      
        1 cgatgcgcga tgccttccgc aaactgacgg cggacgagac agccaagcgc ctcgatgcgg
       61 tgcgcgacta tgcgatcgcc ctgccgtcgg ccaccgacag gaccggcacg attggtttct
      121 gctggggcgg tggcgccagc ttcgcctacg cggcccatca acccaagctc aacgccgccg
      181 tcgtcttcta cggcacaccc ccggccaagg aaacgctggc caacattgcc tgccccgtct
      241 acggtttcta tggcaaggac gacaaccgcg tcacgtcaac agtcgaaccg acgaagcagg
      301 ccatgtcggc gttgaataag ccgtacgagc cacacatcta cgaaggcgcc ggccacggat
      361 tcatgcgcca gcaggacggc cgcgacggcg ccaaccagaa agccgccgac gccgcgtggc
      421 cgcaggcgat tggtttcttg aagaatcacc tgaagtagat catgcaagcg atgatggacc
      481 tcaacgcgct gcgacaacgc ctggacgcgg agcggcgcac gctgctccgc cgcggggaaa
      541 tggttgaagt gcttcggcac ctcacgcgcg tgcagcggga cacgcagcat gagatttctt
      601 tcgcgtcttt gccgtcggcc ggcgccgacg cgatcatcgc tgagcagatc gagcactacc
      661 gaaacctgaa tacgccggtg gagtggaagg tgtacgcgca tgaccagccg cacgatctgc
      721 gacatcggct catcgcgaat ggattttcgc gcggccctga agaagccgtg ctggtgctgg
      781 accttcgcaa tccgccggaa tggatttcag ccgccgcggc ggatgcgccg gtcgtgcgcg
      841 tgacgacgga cgaccaagtc aagatgttcc gcgacgccgc cgcggagatc ttcgccaagg
      901 actacgactg gaccgccggt cagctgcgct acggcatcgc cgctcgatcg accgagcatc
      961 tcggctacat cgcgatcgaa ggtaatcacg cgatcagcat cggccgccta tacacgcacc
     1021 acgaccgcca tttcggcggc ctgtacggcg gcggcacgcg tgcgacccac cgcggacgcg
     1081 ggctataccg cgccgttgtc gcggcccgcg cgcgcgatgc aattgaactg ggcgcgcgat
     1141 acctcatcgt cgatgcgctg ccgaccagcc aaccgatctt gcggcgactg gggttcatcg
     1201 agttgagcaa gacctggccg tgcacctggc acccgggcag tgaaaggtaa gaacgtttgc
     1261 taatccctca cgccgggttc ggaggtactc cgacgacccg gattctgcgg cgctcgaaga
     1321 gctccgggtc atctccggta cgctccgaac ccggcgtggc agcgcgagct accgagcatc
     1381 caattcaaac cactccgccg cgctttgatg cagttgtacg ttcgtccaca gcgcatcgac
     1441 attcgacggt cgcttgctgt ataggtacac gcatgggtca gccgttgacc ttttaaccct
     1501 agccgccgga accgaccggc aggaggatga aaccaaatga tgatcgttcc gcatgggttc
     1561 aacgagtcct tcatcgcgtt cgcgcactgc agcgctcgcg cgcggcgcgc gacgcgtcac
     1621 atcacttctg gatcgaaggc gttcgccaat tcgtgcaggc ggtcgacgcg gggctgacgt
     1681 tcgagaccgt cgtcatcagc ccgatcctgc tgcagagcga cctggccgac atgctggccc
     1741 gccgcctgca gacgcagcgc gtgccgcggg cgcgcttgac acccgaacaa ttcagatcga
     1801 tcagcaccac cgtccgggcg tcggggatcg gcgcgatcgt tcgacagcac tattcgccgc
     1861 tgagcagcct ggatgcgcgc gcggggcttt gctggctggt catcgagcga ttgcgctcgc
     1921 cgggtaacct tgggacaatt ctcagacccg ccgaagccgt cggggcggcg ggcgtgatct
     1981 tcctcaccag cgcgtgcgat cccttcgacc ccgccgccgt gcgcgccagc atgggtggca
     2041 tcttccatct ccgcctggca gtctgctcgc cgcggcaata tcgccaatgg gcgaagcgac
     2101 atggcgtgac gacggcgggc ctggcgccgc gcgtgcggca gctgtggacg cagctaccgg
     2161 cggcgcggcc gatcgccatc ctgctcggcg aggaacgccg cgggctgact atcgagacgg
     2221 ccggattgtg cgacctgacg gtgcaactgc cgatgagcgg gcgggcggat tcgctgaatg
     2281 tggcgatcgc cgcgggcgtg atgctgtacg aattggtgcg acggcagcac ttgcccgggc
     2341 ttgtcgtgta accgacaaca cagatcgagc atccaactcg aacgtccaaa tattgttttg
     2401 gccaccgatg gggcaccgat aaacaccgat gaagagcgat tcgtcgacgg c
//
LOCUS       DATIMH010000598         4278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1880887, whole genome shotgun sequence.
ACCESSION   DATIMH010000598 DATIMH010000000
VERSION     DATIMH010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880887"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VLI90_10350"
     CDS             complement(<1..584)
                     /locus_tag="VLI90_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14652.1"
                     /translation="MESRQLMSATITRLELVNADTGKDIEALKSGITLNLATLPTRHL
                     NVRADVGSGTLSVKFGLDSNSNYRVESSLPLALAGDDGKGHYYAWTPSLGKHTLTATP
                     YLAKFAQGTAGAATSIAFTVTNVAPPAPVGPPINSNWSMKFDEEFNGPLSSNVWTQTL
                     WGDNQAPGDAETYDPSATSVSNGLLSISARKQTLV"
     gene            complement(870..1328)
                     /locus_tag="VLI90_10355"
     CDS             complement(870..1328)
                     /locus_tag="VLI90_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14653.1"
                     /translation="MPPIGQPESFYARQVQYADKMCDPHLHEAFKVGQYITLALDNGL
                     RWESKLKYFDHALRRHCQPPPLPDEDVWLFYRQLSDLVRQYCGEQALKLASKMDDEFA
                     RRVGKGEPRENISEEAEKFFQRLMGLGWDKPNHFNDEDWHQLKLIRDQWV"
     gene            1693..2163
                     /locus_tag="VLI90_10360"
     CDS             1693..2163
                     /locus_tag="VLI90_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14654.1"
                     /translation="MAANQSRPLRLIFNPAKELAARLTQLEAARTDLLADDEYASWRS
                     SMLSEITGRPRMPRSRQITLALCGIACVGLLVLGVFRQSRDAIIGGAAGLIGAALLWF
                     TFAYQYSVMGHLDRDNRLSVIDRLVEMRLVAEPEAADLRAKVRSAFPAQAGTLD"
     gene            2183..2479
                     /locus_tag="VLI90_10365"
     CDS             2183..2479
                     /locus_tag="VLI90_10365"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HSV14655.1"
                     /translation="MLIVINEFHIEPSKIKQATEAAKAAQAQIAKLGHKPARILTDVT
                     GEFYTLMFVAEFESLSAMEKTMEGVMSNPEFQKAYQPLRDAMRSGSRRIMRVVG"
     gene            complement(2853..3164)
                     /locus_tag="VLI90_10370"
     CDS             complement(2853..3164)
                     /locus_tag="VLI90_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14656.1"
                     /translation="MEPIATDKRWIAELVDCHLPTGPALAGSLCQRDAGVADVTCGAR
                     GIGQAVNRFQLLGRNAPMLQDMFDEDFSSLFCHLGDGGENFGRMLGFCHATSSEKQQF
                     L"
     gene            3261..3527
                     /locus_tag="VLI90_10375"
     CDS             3261..3527
                     /locus_tag="VLI90_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14657.1"
                     /translation="MQIDHRVPYQIAGEALVKVKSDPKLVAAAREWRDRWLERVNAGQ
                     YLPAAEGKYEVSRALSDAGAGQVEHAMPRLAGPGSTGSAQAMAA"
     gene            complement(3531..>4278)
                     /locus_tag="VLI90_10380"
     CDS             complement(3531..>4278)
                     /locus_tag="VLI90_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14658.1"
                     /translation="IIDHAKQRKLRVILMPIVLLDNPRGNEWRGTLKPDSWEDWFDSY
                     RDMIGQFAWIAQGHNVDVLVVGSELVSAEGKLDQWTQTIKKVRETFKGNLTYSANWDH
                     YTSIPFWDQLDLVGMNSYYKLGDDQNVSTKEIEDRWREIQGDLLTFQRKVAKPLLFLE
                     VGWCSLANAASEPWDYTKTEEPVDNDLQKRLYEAYFNVWYGNPALGGAMIWEWPPDDS
                     PNHDRGYTPQGKPAEEVLKKWFAKPAWTVK"
BASE COUNT          812 a         1255 c         1350 g          861 t
ORIGIN      
        1 acgagcgttt gcttgcgcgc gctgatcgac agtaggccgt tgctcacgct cgtcgcgctg
       61 gggtcgtacg tctcggcatc gccgggggct tggttgtcgc cccacaacgt ctgcgtccag
      121 acgttgctgg acagtgggcc gttgaattct tcgtcaaact tcatcgacca gttgctgttg
      181 atcggcggtc ccaccggcgc gggtggggca acgttggtga cggtgaaggc gatcgaagtc
      241 gccgcccccg cggtgccttg ggcgaacttt gccaggtacg gcgttgcggt cagcgtgtgc
      301 ttgccgaggc tgggcgtcca ggcgtaatag tggcccttgc catcatcgcc cgccagcgcg
      361 agcggcagcg agctttcgac gcgatagttc gaattcgaat cgagcccgaa cttcacgctc
      421 agcgtgcccg agccaacatc cgcgcgcacg ttcagatgcc tggtcggcag cgtcgccagg
      481 ttcaacgtga tgccgctctt gagcgcctcg atatctttgc cggtgtcggc gttgaccagc
      541 tccagccgcg tgatcgtcgc cgacatcagc tggcgcgatt ccaatgattc caggacgacc
      601 gccggcctat caggcgaacc agcgcatcca cgcccagccg cggattttaa cttgttcatg
      661 tacatctccc actacctttg gccggacaat atacggcagc gcccaggcag aacctaggga
      721 atctgtgcgg ttttccggca aaaaagttca aagtgcggcg cgaccatgcg gcgtgggcaa
      781 gattggtaaa caatcggcga agacgtcact gtgacacggg ccggaggtcc gtgttcatga
      841 taaaggttca gagggcgcgt gggaattgct tacacccatt gatctcggat cagttttagc
      901 tgatgccaat cttcgtcgtt gaaatgattc ggcttgtccc agcccagccc catcaggcgt
      961 tggaaaaatt tctccgcctc ttccgaaatg ttttcgcgcg gttcgccttt gccgacgcgg
     1021 cgggcgaatt cgtcgtccat cttgctcgcc agcttcagcg cttgctcgcc gcagtattgc
     1081 ctgaccaggt ccgacagctg gcggtaaaag agccagacgt cttcgtccgg cagcggtggg
     1141 ggttggcagt ggcggcgcag ggcgtggtcg aaatatttta atttcgactc ccaacgcaag
     1201 ccgttgtcga gtgctagggt gatgtattgg ccgaccttga acgcctcgtg cagatgcgga
     1261 tcgcacatct tgtcggcgta ctggacctgc cgggcgtaga acgactccgg ctgcccgatg
     1321 ggcggcatgg gaggaagttg ccgggcctgc ggcatagcga agccctcgcg taaacgaact
     1381 caccctgttg cgttacgcgc gctggatgct gctgcgtcac gcgcgcgggc cggcgacaaa
     1441 cgacgtgata actcagccag cctgcaagtt gactgaatct taccttaacg gaccacgcgc
     1501 tgacaacgca aagtgcggtg ggaatcagcg gaaattgcgt ggcgcgtcga gcggctttcc
     1561 gtggcggcac tcgctttccc gcttctcata taaaactcac gccggtcagc aagttcgggg
     1621 tatgatgctt tcgacagcga cttctcgtgg aatcagcgcg gaagaatgct cgtccaacct
     1681 tcgaggcttg atatggccgc gaaccagagc agaccgttgc gtctgatctt caatcccgcc
     1741 aaagaactcg ctgcgcgcct gacccaactc gaagcggcgc gcaccgacct gctggcggat
     1801 gacgaatacg cgagttggcg aagcagcatg ctgagcgaga tcaccggccg gccgcggatg
     1861 ccgcgctcgc ggcagatcac gctcgcgctt tgcggcatcg cctgcgttgg tctgctcgtg
     1921 ctcggcgtct tccggcaaag ccgcgacgcg atcatcggtg gcgccgccgg tctgatcggc
     1981 gctgccttgc tctggttcac cttcgcctac cagtattccg tgatgggcca cctcgatcgc
     2041 gacaaccggc tgagcgtcat cgaccggctg gttgaaatgc gtctcgtcgc ggaacccgaa
     2101 gcggcggatt tgcgcgcgaa ggttcgcagc gcgttccctg cccaagcggg tacactggat
     2161 tgaggtatca aaggagattg acatgctgat cgtgatcaac gaattccaca tcgaaccgtc
     2221 caagatcaaa caggcgaccg aagccgccaa ggctgcgcag gcgcagattg ccaagctcgg
     2281 tcacaagccg gccaggatcc tcacggatgt gacgggcgag ttttacacgc tgatgttcgt
     2341 cgccgagttc gagagcctgt ccgccatgga aaagacgatg gaaggcgtga tgtcaaatcc
     2401 cgagttccag aaggcgtacc agccgttgag agatgccatg cgatccggct cacgacggat
     2461 catgcgcgtg gttgggtgat cgagcaaacc ccggcgacaa agcgggtcag tgcagtcccg
     2521 gcgttgacga cgacagagga gggacggggg tcaggtactg aggcaaccaa ccggcggggg
     2581 gatgaacggt cctcagcgtc ttgcaccatc cggcgtctcc gcgtgtgcgt gtgcgcgcgc
     2641 aaaagccatc ctctgcggcg cggcgagatg aatcggatat cggccatgcg gccagcggga
     2701 ctcaaacctg cggaaaccgc gtcgccgggc gatcaactca agaaacgctc ctcggacgcc
     2761 atggcgtgac atacgaatcc ctgtacatct ccgaatcatt gttggaacac atcgaccatc
     2821 tgccacgatt gctgattgag cctgcaccgt cgtcagagga attgctgctt ttctgacgac
     2881 gttgcatggc aaaagccaag cattcggcca aaattctcgc cgccgtcgcc aaggtgacaa
     2941 aaaagcgagg aaaaatcctc gtcgaacata tcctgaagca tgggagcgtt tcgaccgagg
     3001 agttggaaac gatttacggc ctgaccgatg ccgcgagcgc cgcacgtgac gtccgcgacg
     3061 ccggcgtccc gctggcaaag acttccggca agcgcaggac cggtaggcaa atggcaatct
     3121 acaagttcgg cgatccatcg cttatccgtg gcgatcggtt ccaaggacga agagcgttcg
     3181 caaagggctt caaagaaact ctcatcaaca agtacggttc aaagtgcgct ttatgctcca
     3241 cgccctacga gcctcgctat ttgcagatcg atcaccgcgt tccatatcag attgccggcg
     3301 aagcgctggt gaaggtgaag agcgatccga agctggtggc ggcggcgcgg gagtggcggg
     3361 atcggtggtt ggagcgggtg aacgcggggc agtatctgcc cgcggcggag gggaagtatg
     3421 aggtcagtcg cgcgctgagc gatgcggggg ccgggcaggt ggaacacgcg atgccgcgtt
     3481 tggcgggacc gggttcgacg ggctcggcgc aggcgatggc ggcttgagat tcacttgaca
     3541 gtccacgccg gcttggcgaa ccacttcttg agcacctctt ccgccggctt gccttgtggc
     3601 gtgtacccgc ggtcgtggtt ggggctgtcg tccggcggcc attcccagat catcgcgccg
     3661 ccaagggcgg ggttgccata ccagacatta aaatacgcct cgtacagccg cttctgcagg
     3721 tcgttgtcca ccggctcttc ggtcttggtg taatcccacg gctcactcgc cgcgttggcg
     3781 aggctgcacc agccgacttc taaaaacaac agcggcttgg cgaccttgcg ctggaacgtg
     3841 agcaggtcgc cttggatttc gcgccagcgg tcttcgattt ccttggtgct gacgttctga
     3901 tcgtcgccga gcttgtagta gctgttcatg ccgaccaggt cgagttggtc ccagaacggg
     3961 atcgacgtgt agtggtccca gttggcggag tacgtgaggt tgcccttgaa cgtctcgcgg
     4021 actttcttga tcgtctgcgt ccactgatcg agcttgcctt cagcgctgac cagttcggag
     4081 ccgacgacga ggacatcgac gttgtggcct tgcgcgatcc acgcgaactg gccgatcatg
     4141 tcgcggtagc tgtcgaacca gtcttcccag ctgtcgggct tgagcgtgcc gcgccactcg
     4201 ttgccgcgcg ggttatcgag cagcacgatc ggcatgagga tcacgcggag cttgcgctgc
     4261 ttggcgtggt cgatgatg
//
LOCUS       DATIMH010000599         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1304198, whole genome shotgun sequence.
ACCESSION   DATIMH010000599 DATIMH010000000
VERSION     DATIMH010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304198"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VLI90_10385"
     CDS             complement(<1..945)
                     /locus_tag="VLI90_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14659.1"
                     /translation="MLHMSDLIQIHGREQSLLDPNLPDGGLPPVVGVQSFQVFRASRD
                     TEPDGIGFTYHHHVDMACWRGRLYVAWNSCERDEDVWPSRELFSTSIDGANWSAPAEL
                     FPQGISTALRMYFFHAPNGRMLAIAGLRVSTEEVEENKKGSLVVREIRDDHTLGPVYL
                     LQSFNGSDERVKMFTDAGDDGFIAACTQLLDDRVFLEQQDRGRLLGPRRMRWHEASNW
                     PDGVVPGDSAKWVSGKAFSFYRRPDGAIIGVSKMGWSTISRDNGQTWDRWVVPPTLIT
                     GKAKVWSQQTADGRFALVYNPSRRNRFPLIAVSGDDGVN"
     gene            complement(999..>2563)
                     /locus_tag="VLI90_10390"
     CDS             complement(999..>2563)
                     /locus_tag="VLI90_10390"
                     /inference="COORDINATES: protein motif:HMM:NF045341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14660.1"
                     /translation="DAATLVLVAATDFVNFQDISANPAKRCQQMLAKVASKSYEALRD
                     DHVADHQALFNRVKLDVGRTDRADLPTDQRLKAGLDGDPALAALFFQFGRYMLISSSR
                     PGSQPANLQGVWNELLNPPWESKYTTNINLEMNYWPVETTNLSECHQPLFDMINDLTI
                     SGGRTAKDLYHSRGWVLHHNTDNWRGTAPINNIDGVWPTGGAWLCSHLWEHYLFTGDE
                     HFLAASYPAMKGAALFFVDSLVKDPHTGYLVTNPSFSPEQGTLCAGPAMDMQLIRALF
                     DSTIAASKVLKTDQEFADQLTALRPRLAPDKIGQHGQLQEWQDDVDQPNNNHRHMSPL
                     WGLYPGAQFTPNEPKLFDAAKVLLKWRGDGSTGWSYAWRIPLWARVNDGDFALRQLSL
                     QLAKRTCPNLFDKCGPFQVDGNFGATAGVAEMLLQSHLHPAKEPDVQEIDLLPALPKA
                     WASGSVSGLCARGGFEVDMNWKDGALTHAVVRSKLGRPCEIHAGSKKLSLQTEAGKTY
                     TFDAALQRLASN"
BASE COUNT          457 a          852 c          776 g          478 t
ORIGIN      
        1 gttgacgccg tcgtcaccgc tgacggcgat cagcggaaaa cgattccgcc gcgacgggtt
       61 gtagaccaac gcgaaccgcc cgtcggccgt ttgctgcgac cagaccttgg ccttgccggt
      121 aatgagcgtc ggcgggacga cccagcggtc ccacgtctgg ccgttgtcgc gcgagatcgt
      181 gctccatccc atcttgctga cgccgatgat cgcgccatcg ggccggcgat agaatgaaaa
      241 ggccttgccc gagacccact tcgcgctgtc gccgggcacg acaccatcgg gccagttcga
      301 cgcctcgtgc caccgcatcc gccgcgggcc gagcaatcgc ccgcggtcct gctgttcgag
      361 gaagacgcgg tcatcgagca gttgcgtgca cgcggcgatg aacccgtcgt caccggcatc
      421 ggtgaacatc ttcacgcgct cgtcagaccc attgaacgat tgcagcagat agacgggccc
      481 gagcgtgtga tcgtcgcgaa tctcgcggac gaccaacgag cccttcttgt tctcctcgac
      541 ttcctcggtg ctgacgcgca gccccgcgat cgccagcatc cggccattgg gcgcgtggaa
      601 gaagtacatg cgcagcgcag tcgatatgcc ctgcggaaac agttccgccg gcgcggacca
      661 gttcgcgccg tcgatggatg tgctgaacag ctcgcgcgac ggccagacgt cttcatcgcg
      721 ctcgcagctg ttccacgcga cgtagagccg gccgcgccag caggccatat cgacgtgatg
      781 gtggtacgtg aaaccgatgc cgtcgggttc ggtgtcgcgc gaggcgcgaa agacctgaaa
      841 gctctgcacg ccgaccaccg gcggcaagcc gccatctggc aggttggggt cgagcagcga
      901 ttgctctcgg ccgtggattt gaatgaggtc ggacatatgt agcacgggcg tctcgcccgc
      961 gtcagggcaa ctcggcacgg gctctggcgt ccgtgctact aattcgacgc cagtcgctga
     1021 agcgccgcat cgaacgtgta cgtcttaccc gcctcggtct gaagcgacag ttttttcgac
     1081 cccgcgtgaa tctcacacgg ccggccaagc ttcgatcgca ccaccgcgtg cgtcagcgcg
     1141 ccgtccttcc aattcatatc cacttcaaat cccccacgcg cgcacaagcc tgagaccgac
     1201 ccactcgccc acgcctttgg cagcgccggg agcagatcga tctcctgcac gtccggctct
     1261 ttggccggat gcaggtggct ctgcagcagc atctccgcca cgccggccgt cgcgccgaag
     1321 ttgccgtcca cctggaacgg cccgcacttg tcgaacaggt tcgggcacgt tcgcttggcc
     1381 agttgaagcg acagctgccg cagcgcgaag tcgccgtcgt tcacgcgggc ccacagcggg
     1441 atgcgccacg cgtagctcca cccggtgctg ccgtcgccac gccacttcag cagcaccttc
     1501 gccgcgtcga acagtttggg ttcgttcggg gtgaactgcg cacccgggta caacccccac
     1561 aacggcgaca tgtgccggtg attgttgttc ggctgatcga cgtcatcctg ccattcctga
     1621 agctgcccat gctggccgat cttgtccggc gcgagccgcg gtcgaagcgc cgtcaattga
     1681 tccgcgaatt cctgatcggt cttgaggacc ttcgacgcgg cgatcgtcga atcgaacagc
     1741 gcccggatca actgcatatc catcgccggc cccgcgcaca gcgttccctg ttccggtgag
     1801 aacgacggat tggtgacgag ataacccgta tgcggatctt taaccagcga atcgacaaag
     1861 aacagcgctg cccctttcat cgccggatat gacgcggcca aaaaatgttc gtcgccggtg
     1921 aacaggtaat gctcccacag gtgcgagcag agccacgcgc caccggtggg ccagacgccg
     1981 tcgatgttgt tgatcggcgc ggtgccgcgc cagttgtcgg tgttgtgatg cagcacccac
     2041 ccgcgggaat gatacaggtc ctttgcggtt cgcccgccgc tgatcgtcag gtcgttgatc
     2101 atgtcgaaca gcggctgatg gcattcgctc aggttcgtcg tctcgaccgg ccaatagttc
     2161 atctcgaggt tgatgttggt cgtgtacttg ctctcccacg gtggattgag caactcgttc
     2221 cacacaccct gcaaattcgc cggctgcgag cccgggcgac tgctggagat cagcatgtac
     2281 cgcccgaact ggaagaacag cgccgccagc gccggatcgc catccagccc cgccttcaat
     2341 cgctggtccg ttggtaaatc cgcgcgatcc gttcgaccga cgtccaactt cacgcgattg
     2401 aaaagcgcct gatgatcggc cacatgatcg tcgcgaagcg cttcgtacga cttgctcgcg
     2461 accttggcga gcatctgctg gcagcgcttg gcaggattgg cgctgatgtc ctggaagttg
     2521 acgaagtcgg tcgcggccac cagcaccagc gtggcggcat cgg
//
LOCUS       DATIMH010000600         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1591305, whole genome shotgun sequence.
ACCESSION   DATIMH010000600 DATIMH010000000
VERSION     DATIMH010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591305"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VLI90_10395"
     CDS             complement(<1..802)
                     /locus_tag="VLI90_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HSV14661.1"
                     /translation="MPSTAERISATYRIETPLAIERAADVLAGEQSSGTFVAVPGETD
                     ALRDRFRARVERVTELEPAREPSLPGSRSKSSPPATYRRAEVTVSWSLENVGYNLPAL
                     VSTIQGNLYELAEFSGLKLIDLEVPPSFARHFTGPRFGIEGTRRMTGVGDRPMIGTII
                     KPSVGLSPEQTAELVAQLVQAGIDFIKDDELMADPPHSPFDARVDAVMRVINDHAQRT
                     GKRVMYAFNVSDEIDAMMRHYDAVARSGNTGVMVSMNSVGLAGMKKLCD"
     gene            1094..>3057
                     /locus_tag="VLI90_10400"
     CDS             1094..>3057
                     /locus_tag="VLI90_10400"
                     /inference="COORDINATES: protein motif:HMM:NF039200.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HSV14662.1"
                     /translation="MLDRLDQYNVTWDTPSTGTSGSMPLGNGDVGVNVWVEPGGDVLL
                     LIAKSDAWDENSINLKLGRVRLRFTPNPLGDRAPFRQALRLKTGDIEIGLGQLRVHIW
                     VDANAPVIRIETESERPFEMQATLEIWRTTPRQIKTQTSDMFKNLAGKNADPYPTIVT
                     PDHVLSADNRIVWCHHNEVRHPDPYAVNMVLQGLGEVMATMPHPLLGRTFGAAIVGDG
                     FALRDPHLLCSSQPSRTHRLNIHAFTSAPPRSGRPAGALVSAENPKTVPLRDDRYAAT
                     ATIDEWQRQLDQNIAQDRADRANHESWWSDFWNRSWIYITSNLPEEQASTFQITQAYI
                     LCRFMNAAAGRGASPIKHNGSLFSVGQGDDPDFRRWGGPGFWFQNQRLIYWPMLAAGD
                     FDFVRPWLDMYTSMLPLQRQRTAKYFHHGGAHYPETVTFWGAEVSAHYEWIPFEQRES
                     PEAGSPYLKYYWTGGVELSLMLCEYFAYTNDRAFAQAALVPVASAVLEFFDLHYPRDA
                     DGRIRFEPAQSLETWHEAINPLPEIAGLRCVLTKMLALPQDLTSPTDRQRWTRTLDEL
                     PPIPIGERDGKRVILPAERFDKKKNTENPELYCVFPYRLFGIGKPDLQLARDTFAARL
                     HKSHDCWSQDDIQMALLGLTDEAKQSVTNRA"
BASE COUNT          581 a          991 c          924 g          561 t
ORIGIN      
        1 ggtcgcagag ttttttcatg ccggccagtc cgacgctgtt catgctgacc atcacgccgg
       61 tgttgcccga ccgggcgacg gcgtcgtaat gccgcatcat cgcgtcgatc tcgtcgctca
      121 cgttgaacgc gtacatcacg cgcttgccgg tgcgctgggc gtggtcgttg atcacgcgca
      181 tcaccgcgtc gacccgcgca tcaaacggcg agtgcggcgg gtccgccatc agctcgtcat
      241 ccttgatgaa gtcaatgccg gcttgcacca gttgcgccac cagctccgcg gtctgctccg
      301 gcgacagccc gacgctcggt tttataatcg tgccgatcat tggccggtcg ccaacgccgg
      361 tcattcgccg cgtgccttcg atgccaaagc ggggacccgt gaagtgccgc gcgaaggacg
      421 gcggcacctc caggtcgatc aacttcagcc cggaaaactc ggccaattcg tacaggttcc
      481 cctgaatcgt cgagaccagc gccggcaggt tatagccgac gttttccagc gaccacgaaa
      541 cagtcacttc cgcccgccga tacgtcgccg gcggggatga tttactccga ctgcccggca
      601 agctcggctc acgcgccggt tccaattccg tgacgcgttc gaccctcgcc cgaaatcgat
      661 cgcgcagcgc atcggtttcc cccggcaccg caacaaatgt ccccgacgac tgctcccccg
      721 caagcacgtc tgccgcccgc tcgatcgcca gcggcgtctc gatgcggtac gttgccgaaa
      781 tgcgctccgc ggttgaaggc atcttcacat tgtcctgcgc caggcagcgc gatccaatta
      841 tatttaatgt gtaaaccgtt gcattcgacc ggcaaagtct ggtcccctct cccacgtacg
      901 cgtgggagag ggttagggtg agggtgattt ggagctgtga atgaccaacg tccaaatgcc
      961 aaatcccaat gaagtcccaa ggcccaatgc tcaagcgcgc cggttgggct ttggtcattg
     1021 gaacttcatc gggatttgga gctcgagctt tggttattga cccatcgaaa tcaccgtcac
     1081 cgtaagagtc gccatgctcg atcgcctcga ccaatacaac gtcacctggg acacgcccag
     1141 caccggcacg tctggctcga tgccgctcgg caacggcgac gtcggcgtca acgtctgggt
     1201 cgaacctggc ggcgacgtgc tgctgctgat cgccaagagc gacgcgtggg atgagaactc
     1261 gatcaacctg aaactgggcc gcgtgcgact gcggttcacg ccgaacccgc tcggcgatcg
     1321 tgcgccgttt cgccaggcgc ttcgtttgaa gaccggcgac atcgaaatcg ggttgggcca
     1381 gctgcgcgtt cacatttggg tcgatgccaa cgcgccggtc attcgaatcg aaacggaaag
     1441 cgagcgaccg tttgagatgc aggcaacgct ggagatctgg cggacgacgc cgcggcagat
     1501 caagacgcag accagcgata tgttcaagaa cctcgcgggg aagaacgccg atccgtatcc
     1561 gacgatcgtc acgccggacc acgtgttgag cgccgacaac cggatcgtct ggtgccatca
     1621 caatgaagtg cgccatcccg atccgtacgc ggtgaacatg gtgctgcagg ggctgggcga
     1681 ggtaatggcc acgatgccgc acccgctgct cggtcgcacg ttcggggcgg cgatcgtcgg
     1741 cgacggtttt gcgctgcgcg atccgcacct gctttgttca tcgcaaccgt cgcgcacgca
     1801 ccggctcaac atccacgcgt tcacgtcagc tccgccgcgt agcggccgcc ccgcaggggc
     1861 acttgtttcc gccgaaaatc cgaagaccgt gcccctgcgg gacgaccgct acgcggcgac
     1921 cgcaacgatc gacgagtggc agagacaact cgatcagaac attgcgcaag atcgcgccga
     1981 ccgcgcgaat cacgagagct ggtggtccga cttctggaac cgcagctgga tctacatcac
     2041 atccaatttg cctgaagagc aggcgagcac gtttcagatc acgcaggcct acatcctctg
     2101 ccgcttcatg aacgccgccg cggggcgcgg ggcgtcgccg atcaagcaca acggctcgct
     2161 cttcagcgtc gggcagggcg acgatccgga cttccgtcgc tggggtggac ctgggttttg
     2221 gttccagaac cagcggttga tttattggcc gatgctcgcg gcgggcgact tcgattttgt
     2281 gcggccatgg ctcgacatgt acacgagcat gttgccgctg cagcggcagc gcacggcgaa
     2341 gtattttcat cacggcggcg cgcactatcc cgagaccgtc acgttctggg gcgcggaggt
     2401 cagcgcccat tacgaatgga tcccgttcga gcagcgggaa agcccggagg ccggctcgcc
     2461 gtatctcaag tactactgga ccggcggcgt cgaactgtcg ctgatgctgt gcgagtattt
     2521 cgcgtacacc aacgatcgcg catttgcgca agccgcactt gtgccggtcg cgtcggcggt
     2581 gctggagttc ttcgacctgc actacccgcg cgacgccgat ggacgcattc gattcgagcc
     2641 cgcgcagtcg ttggagacat ggcacgaggc aatcaatccc ctgccggaga tcgcggggct
     2701 gcgctgtgtg ctgacgaaga tgctcgcgct gccgcaggat ctcacgtcgc ccaccgatcg
     2761 acagcgctgg acgcgcacgc tcgatgaact gccgccgatc ccaatcggcg agcgtgacgg
     2821 caagcgcgtc atcctccccg ccgaacgttt cgacaaaaag aagaacaccg aaaacccaga
     2881 gctgtactgc gtcttcccgt atcgcctgtt cggcatcggc aagccagacc tgcaactggc
     2941 ccgcgacacg ttcgccgcgc gcttgcacaa atcgcacgat tgctggtcgc aggacgacat
     3001 ccagatggcg ctgcttgggt tgacggacga ggcgaagcag tcggtcacaa accgcgc
//
LOCUS       DATIMH010000601         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4186357, whole genome shotgun sequence.
ACCESSION   DATIMH010000601 DATIMH010000000
VERSION     DATIMH010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4186357"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1229)
                     /locus_tag="VLI90_10405"
     CDS             complement(204..1229)
                     /locus_tag="VLI90_10405"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV14663.1"
                     /translation="MFNSDNGKPRVIVVTGASGGVGRAVVRQLGQQRAKIGLLARGRD
                     ALEGAKRDVEQAGGEGLVLPTDVSQFDQVEAAAKAVEERFGPIDVWINDAMVSMYSPF
                     MQMKPEEFKHIVEVTFLGQVYGTRCALQRMVPRDRGVIIQVGSALAYRSIPLQSAYCA
                     SKHAIGGFTESVRSELIHMKSNVRISMVNLPGVNTTQFEWTKNNLPHKPRPTGPIFQP
                     EVAADAICWTIDHDRRQVDVGWPTVEAVVGEKLIPGTLDHYLAHAAWEGAQLPEPADP
                     NHKDNFWQPLPGDHGSHGPFDAQAKEHSTQLWATKHRTGLLFAAAGLVAAAGFAVARR
                     NDNGWHS"
     gene            complement(1298..>3377)
                     /locus_tag="VLI90_10410"
     CDS             complement(1298..>3377)
                     /locus_tag="VLI90_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSV14664.1"
                     /translation="FLLVEFGGDSKQDSDAQAKRFMEHMKQSAGDKAPHMKLYDDPQQ
                     EEMIWKVRQGSLGSTAWVPGMPDTWEGWEDSAVPVPKIGDYLRDLRQLLDKYDYHPTL
                     YGHLGQGCVHTRIPFDLYTQPGIEKWRSFINEATDLVVKYGGSFSGEHGDGQSRGEWL
                     PKMFSPTLMEAMREFKAIWDPQGKMNPGKAAVDPYKMAENLRIGPDYNPPQPQTHFAY
                     PADRHSFARASLRCVGVGECRRHGEQTMCPSYMVTREEMHSTRGRARVLFEMMNGEII
                     DDGWRSEEVKEALDLCLSCKGCKGDCPVNVDMATYKAEFLSHYWAGRLRPRHAFAFGW
                     IHIWSRLASVAPMLANFVTQTPGVRVLAKWMAGMDQRRTIPAFAPQTFKDWFKAHEPK
                     NAAGEPVLLFADTFNNFYHPDTAIAAVEVLEDAGFRVQVPMADLCCGRPLYDYGFLGM
                     ARRWWQHMLRELQPQIEAGMPMVVLEPSCWAAFKDELTNIIAEDQDAKRLQDQTYTLA
                     DFLEKRAPHYRLPKLARKALLHGHCHQKALDKLNDKQYGKMFNEKKVLSQMGVDHREP
                     ETGCCGMAGAFGFEAENGHRDISVKCGERVLLPETRKAGDDELIIADGFSCREQIEQC
                     TDRQAMHIAQVMAMALRDSGSGTDERPEMAMVRQKKREFRRAAVRTASFFAAGAVLGA
                     VVYTRKKRFS"
BASE COUNT          553 a         1163 c          992 g          669 t
ORIGIN      
        1 cgaaggatct ggccgagaag tcagtgaacg tctgctttcg aaaccgacac cgcggtcgct
       61 cgcggtttcg cagacggaca agccgcggtt ctcgatctgc gaaaccgcaa gcgactgctg
      121 taccgttcgt gtccgtgaac cttcacttac ttctcagcca gatccttcga ctccgcggct
      181 gcgccgccgc gctcaggatg aactcacgaa tgccacccat tgtcattccg ccgcgccacg
      241 gcaaaccccg ccgcggcgac cagtccggcg gcggcgaaga gcaatcccgt gcgatgcttc
      301 gtcgcccaca gctgcgtgct gtgctccttc gcctgcgcat cgaacgggcc gtgtgagccg
      361 tgatcgcccg gcaggggttg ccagaagttg tccttgtgat tcgggtcggc cggctcgggc
      421 agttgcgcgc cttcccaagc cgcgtgggcc agataatggt ccagcgtgcc gggaatgagc
      481 ttctcgccga ccaccgcttc caccgtcggc cagccgacat ccacctgccg gcggtcgtgg
      541 tcgatcgtcc agcagatcgc gtcggcggcg acctcgggtt gaaagatcgg ccccgtcggc
      601 cgcggtttgt gcggcaggtt gttcttggtc cactcgaact gcgtcgtgtt cacgcccggc
      661 aggtttacca tcgaaatccg cacgttgctc ttcatgtgga tcagttccga ccgcaccgat
      721 tcggtaaagc ctccgatggc gtgcttcgac gcacagtacg ccgactgcag cgggatcgac
      781 cggtacgcca gcgcggagcc gacctggatg atcaccccgc ggtcgcgcgg caccatgcgc
      841 tgcagcgcgc agcgggtgcc gtagacttgg cccaggaacg tcacctcgac gatgtgtttg
      901 aactcctccg gcttcatctg catgaacggc gagtacatcg acaccatcgc gtcgttgatc
      961 cagacgtcga tggggccaaa ccgttcttca acggccttcg ccgccgcttc gacttgatcg
     1021 aactggctca cgtccgtcgg caacactagg ccttcaccgc cggcttgttc gacgtcgcgc
     1081 ttggcgcctt ccaatgcatc gcgcccgcgg gcgagcaggc cgatcttggc gcgctgctgg
     1141 ccgagctggc ggacgacggc gcgcccgacg ccgccactag cgccagtgac gacgatgacg
     1201 cggggttttc cgttatcgga gttaaacatg gcgatctcct tcgtagcacg ggcgtctcgc
     1261 ccgtgcctag cacttcggca cgggcgagac gcccgtgcta cgaaaatcgt ttcttccgcg
     1321 tgtacacaac tgcgccgagc acggcgccgg ctgcgaagaa tgacgcggtg cgaacggccg
     1381 cgcgccggaa ctcgcgcttc ttctgccgca ccatcgccat ctctggccgt tcatccgttc
     1441 cactgcccga atcacgcagc gccatcgcca tcacctgcgc gatgtgcatc gcctgccggt
     1501 ccgtgcactg ctcgatctgc tcgcgacagc tgaagccgtc ggcgatgatc agctcgtcat
     1561 cgcccgcctt ccgcgtctcc ggcagcagca cgcgttcgcc gcacttgacg gagatgtcgc
     1621 ggtggccgtt ctcggcttcg aagccgaacg cgccggccat gccgcagcag ccggtctccg
     1681 gctcgcggtg atcaacgccc atctggctca acaccttctt ctcgttgaac atcttgccgt
     1741 actgcttgtc gttgagctta tcgagcgctt tctggtggca gtgcccgtgc agcagtgcct
     1801 tgcgagccag cttcggcagc cgatagtgcg gcgcgcgctt ctccagaaaa tccgccagcg
     1861 tgtacgtttg atcctgcagc cgcttggcgt cttggtcctc agcgatgatg ttcgtcagtt
     1921 catctttgaa cgccgcccag cagctgggtt cgagcacgac catcggcatg cccgcttcga
     1981 tttgcggctg cagctcgcgc agcatgtgct gccaccaccg ccgcgccatg cccaggaagc
     2041 cgtagtcata cagcggccgc ccgcagcaca agtcggccat cggcacctgc acgcggaagc
     2101 ccgcgtcttc gagcacttcc accgcggcga tggccgtgtc gggatgatag aaattgttga
     2161 acgtgtcggc gaacagcagc accggctcgc ccgccgcatt tttcggttcg tgcgctttga
     2221 accaatcctt aaacgtctgc ggcgcgaatg cagggatggt ccgtcgctga tccatccccg
     2281 ccatccactt ggcaagcacg cgcacgcccg gcgtctgcgt cacaaaattc gccagcatcg
     2341 gcgcgaccga tgccagccgc gaccagatgt gaatccatcc gaacgcgaac gcatgccgcg
     2401 ggcgaagccg gccggcccag taatggctga ggaactccgc cttgtacgtt gccatgtcca
     2461 cgttgaccgg gcaatcgccc ttgcatccct tgcaggacag acacaggtcc agcgcctcct
     2521 tcacctcttc gctgcgccaa ccgtcgtcga tgatctcgcc gttcatcatc tcgaacagca
     2581 cgcgggcccg gccgcgcgtc gagtgcatct cttcgcgcgt caccatgtag ctggggcaca
     2641 tcgtctgctc gccatgccgg cggcattcac ccacacccac gcatcgcagc gacgcgcggg
     2701 cgaagctgtg ccgatcggcc ggataagcaa aatgcgtctg cggttgcggc gggttgtagt
     2761 ccgggccgat gcgcaggttc tccgccatct tgtacggatc caccgcggct ttgcccgggt
     2821 tcatcttccc ctgcggatcc caaatcgcct tgaattcgcg catcgcttcc atcagcgttg
     2881 gcgagaacat cttcggcagc cactcgccgc gcgattgccc gtcgccatgt tcacccgaga
     2941 aactgccgcc gtacttcacg accaggtccg tcgcttcgtt gatgaatgac cgccattttt
     3001 cgatgccggg ttgggtgtac agatcgaacg gaatgcgcgt gtgaacgcag ccctggccca
     3061 ggtggccgta gagcgtgggg tgatagtcgt acttgtccag caactggcga aggtcgcgca
     3121 ggtagtcgcc tatctttggc accggcacgg cggaatcttc ccagccttcc cacgtgtccg
     3181 gcatgcccgg gacccacgcg gtggaaccaa gcgatccttg gcgcaccttc cagatcatct
     3241 cctcctgctg cgggtcgtcg tacagcttca tgtgcggcgc tttgtcgccg gccgactgct
     3301 tcatgtgctc catgaaacgc ttcgcctgag catcgctgtc ttgcttcgaa tcgcccccga
     3361 attcgaccag caggaat
//
LOCUS       DATIMH010000602         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4476364, whole genome shotgun sequence.
ACCESSION   DATIMH010000602 DATIMH010000000
VERSION     DATIMH010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4476364"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..2853)
                     /locus_tag="VLI90_10415"
     CDS             complement(91..2853)
                     /locus_tag="VLI90_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSV14665.1"
                     /translation="MARHELTNFHRRLLVGVIHVVEHPKRMLLIAGLLLLACGALAMW
                     KLDISTDQDQLFSSNVPFFHNYIEFTRDFPENSANYVIIEPKDPAHPPPVDRWTDCAD
                     AITDRLGKETRFVKTVDSHVPIEDLGAWGLLFEDPSALKQDAADAMRMIQLAKLWGEA
                     PGPLTGLLGKSPFERFLSGVGLQPPDVETASFIKLLADGWNRALAETSRPITVGDQVI
                     DLAALGATDPGRLGYYYVADEQNHANHLLLVRVFEQTDYTSMGGLAAAIDGIRAATRE
                     AAQAFPEFNIALTGRPALEADEMLTTDRDSRNSEICALIAVFVGLVVLLRSVWLAVAA
                     EIALLVGIGWTFGWATISIGELNLLSMVFLIALIGIGMDYLIQVLTRYRREAAKHKDP
                     RVIWAGVFKYVAAPINTACLGAAGAFFVSVFTNFRGAAELGIIASGGLLLCLLTCYVI
                     LPALLTIFPVKIRPGEKEKFDLVARSRERSHHWWLTLPALWAVLLLAGIPFARRARFD
                     PSLLNMQAQNLESVKLVRKLQTWSAVVLSKDLDVLRKVRDVIQPLPTVASTESILSAY
                     DNYDWLKQHEREMPSVKWSGPQPIAADDIPRLATKARALADRYRNAAAGSTTQPAVFD
                     ETATAIAAFATTLASGDHAAQAARLTDWQRVFVGHLKDMLAPFHPPPLQINKLPATLR
                     EHLVGSTTGRFALYINPRDDLWVQSNLKAFVHDVERADATVPNAPPVTGIAVNIYHST
                     SSIERSFYHATAYALALIFVLVLIDLRDIPQTMLAISVLAMGLPMLVALMGLFNVSWN
                     FANFFGLPILIGAGHEYGVFMVHRYNEAVSDQRRCWERWDVSDRALLLCAFVTCASFG
                     FFWALGHHQGLKSLGLVMALGIGCIYLATVCMLRPMLLWRLANQKLCRQPPADNRDAV
                     PATEAK"
     gene            complement(3121..3714)
                     /locus_tag="VLI90_10420"
     CDS             complement(3121..3714)
                     /locus_tag="VLI90_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HSV14666.1"
                     /translation="MARSPLPIFAPSRTIAVPPGPVQLIIDRQHFQQIVLDGILKAKT
                     SIDVMTADFKAMLVPDARSSRRRAPSIVEHFRRLAERGVEIRLLHAGTPSSAALVELK
                     RELPVNLTIRRCPRLHAKAVVIDSRAMYLGSANLTGAGLGAKADGRRNFEMGIWTESP
                     TLIDGVLEQFNALWEGRQCPTCKRKDVCPAPLEEPEL"
     gene            4165..4611
                     /locus_tag="VLI90_10425"
     CDS             4165..4611
                     /locus_tag="VLI90_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14667.1"
                     /translation="MPGEDRRRSAPAAVATELASDTRQLTTKARPARRRSVRLPNVLS
                     IGMPEGQPLEGVSHMPVRQPREIIEMLRVLHFNLLLVGPNALEPATWAVLELVHRYWP
                     QLRWVLMPGENTGDREEIMARSLGATSVGTDLRVIVELAGGKPVAV"
     gene            4829..>5198
                     /locus_tag="VLI90_10430"
     CDS             4829..>5198
                     /locus_tag="VLI90_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14668.1"
                     /translation="MGILAGGNARRMLAIAAALTAGLTLASTARAALTLTLSEPGAPG
                     GPISTTITDNGAGDQDLTTGSIEVNDPNFGDFKTNILVGFSNRTDPTAGPVADLEVQS
                     LDVTKNTNVAAATLIITLQDT"
BASE COUNT          969 a         1596 c         1719 g          914 t
ORIGIN      
        1 cacgatgaat gttccagatc cgggtccgtc attttcagac ggaccgcgcc gaggacggac
       61 cggccctgcc ggttgcttgc gtgcgcgaga tcacttggct tcagtggcgg ggaccgcgtc
      121 gcggttatcg gcgggaggtt ggcggcatag cttctgattc gccaatcgcc acagaagcat
      181 cggacgcaac atgcagaccg tcgctaagta gatgcaaccg attccaaggg ccatcaccag
      241 gccaaggctt tttaagcctt gatggtggcc cagcgcccag aagaaaccga agctggcgca
      301 ggtgacgaag gcgcacagca gcagcgcgcg gtcgctgacg tcccatcgtt cccagcagcg
      361 gcgttggtcg cttaccgctt cgttgtaacg gtggaccatg aagacgccgt attcgtgacc
      421 cgcgccgatc aggatcggca ggccgaagaa gttggcgaag ttccagctga cgttgaatag
      481 tcccatcagg gcgacgagca tcggcagtcc catcgccagc acgctgatgg ccagcatcgt
      541 ctgcgggatg tcgcgcaggt cgatcagcac gagcacgaag atcagcgcca gcgcataggc
      601 ggtggcgtga tagaacgagc gctcgatgga gctggtggag tgatagatgt tgacggcgat
      661 gccggtgacg ggcggggcgt tggggacggt ggcgtcggcg cgctccacat cgtgcacgaa
      721 tgcttttagg ttcgactgaa cccacaaatc atcgcgcggg ttgatgtata gcgcgaagcg
      781 gccggtcgtg ctgccgacca ggtgctcgcg cagcgtggcg ggcagcttgt tgatttgcag
      841 cggcggcggg tggaacgggg ccagcatgtc cttgagatgt ccgacgaaga cgcgctgcca
      901 atcggtcagc cgggcggctt gcgccgcgtg atcgccgctg gccaacgtcg tcgcgaaggc
      961 ggcgatcgcc gtggcggttt catcgaagac cgcgggctgc gtggtgctgc cggcggcggc
     1021 gttgcgatat cgatccgcca gcgcgcgcgc cttggtcgcc agccgcggga tgtcatccgc
     1081 ggcgatcggc tgtgggccgg accacttcac gctgggcatc tcgcgctcgt gctgcttgag
     1141 ccaatcgtaa ttgtcgtacg cgctgaggat gctttcggtg gaagccaccg tcggcagcgg
     1201 ttgaatcacg tcgcgcactt tgcgcagcac gtcgagatct ttcgacagga cgacggccga
     1261 ccacgtctgt agcttgcgga ccagcttcac tgactcaagg ttctgcgcct gcatgttcag
     1321 caggctcgga tcgaaccgcg cccggcgcgc gaagggaatc ccggctagca gcagcaccgc
     1381 ccacagcgcc ggcagcgtca gccaccagtg gtgcgatcgc tcgcgcgagc gcgcaacgag
     1441 atcgaacttc tccttctcgc cgggacggat cttcaccggg aagatcgtca acagtgccgg
     1501 caggatcacg tagcaggtga gaaggcacag cagcaatccg ccgctggcga tgatgcccag
     1561 ctcggcggcg ccgcgaaagt tggtgaagac cgacacgaag aacgcgccgg cggcgccgag
     1621 gcaggcggtg ttgatcggcg cggcaacgta tttgaacacg cccgcccaga tcacccgcgg
     1681 gtctttgtgc ttcgcggctt cgcgccgata acgcgtcagc acctggatca ggtaatccat
     1741 gccgatgccg atcagcgcga tgaggaagac catcgacagg aggttcagct caccgatgga
     1801 gatcgtcgcc cagccgaacg tccacccgat gccgacgagc agcgcgatct ccgcggcgac
     1861 cgcgagccag accgagcgaa gcagcacgac cagtccgacg aagaccgcga tcagcgcgca
     1921 aatttctgag ttgcgcgaat cgcgatcggt ggtgagcatc tcgtccgctt ccagcgcggg
     1981 ccggccggtc agggcgatgt tgaattccgg aaatgcctgt gccgcttcgc gcgtggcggc
     2041 gcggatgccg tcgatcgcgg cggccaagcc gcccatcgac gtgtagtcgg tctgctcgaa
     2101 cacgcgcacg agcagcaagt ggttggcgtg attctgctcg tcggcaacgt agtaatagcc
     2161 gagccgaccg gggtcggtcg cgcccagcgc cgccaggtcg atgacctgat cgcccacggt
     2221 gatcggccgg ctcgtctcgg ccagcgctcg attccatccg tctgccaaca gcttgatgaa
     2281 cgacgcggtc tcgacatccg gcggctgaag cccgacgccg gacagaaacc gctcgaacgg
     2341 cgacttgccc agcagtcccg tcagcggccc cggcgcttca ccccacagct tggccagctg
     2401 gatcatccgc atcgcatccg cggcgtcctg cttcagtgcg ctcgggtcct cgaacagcaa
     2461 cccccacgcg cccaggtcct cgattggcac gtggctgtcc accgtcttca caaaccgcgt
     2521 ctctttgcca agccgatcgg tgatcgcatc cgcgcaatcc gtccaccgat ccaccggcgg
     2581 cggatgagcg ggatccttgg gctcgatgat cacgtagttc gcgctgttct cgggaaagtc
     2641 ccgggtgaac tcgatgtagt tgtgaaagaa cggaacattg ctggaaaaca gctggtcctg
     2701 atcggtggag atatcgagct tccacatcgc cagcgcgccg caggcgagca gcagaagccc
     2761 cgcgatgagc agcatgcgct tgggatgctc gaccacgtga atcacgccga ccagcaggcg
     2821 gcgatggaag ttcgtcagct catgacgggc catggctcga ctgtaaggca agcgcggaaa
     2881 aaatcgaagg gaagaacggc cggagccgag ttggcggagc ctttcgtcaa acctggtgtc
     2941 gatggtttca actgaggatt cagcaagagg ctactcatcg ctggcatgcg aaagcgcgct
     3001 ctcgatctgg tcccctctcc cacgtactcg tgggtgaggg ggaaaatgcg ccgacgcgct
     3061 ttatttagct ggcggtcagt agccgccgta agacaaacga tctcgcgcga actcagcgcg
     3121 ctacagctcc ggctcctcca gcggcgccgg gcagacgtcc tttcgtttgc acgtcgggca
     3181 ttgccggcct tcccatagcg cgttgaactg ctcgagcacg ccgtcgatca gcgtgggcga
     3241 ttcggtccat atgcccattt cgaagttgcg gcggccgtcg gctttggcgc ccaggcccgc
     3301 gccggtgaga ttggcgctgc cgaggtacat cgcgcggctg tcgatgacga ccgctttggc
     3361 gtgcagccgc ggacagcggc ggatcgtgag gttgaccggc agctcgcgtt tgagttcgac
     3421 caacgcggcc gacgatggag tgccggcgtg caggaggcgg atttcgacgc cgcgttcggc
     3481 caggcggcgg aagtgctcga cgatagaggg ggcgcgccgc cgcgacgagc gggcgtcggg
     3541 gacgagcatc gctttgaagt cggccgtcat cacgtcgatg ctggttttgg ctttgaggat
     3601 gccatcgagc acgatctgct ggaaatgctg gcgatcgatg atgagctgca ctggtccggg
     3661 cgggaccgcg atggtccgcg agggcgcgaa gatgggcaac ggcgaacgcg ccataatgga
     3721 atcatcgaca tagggcgcat attttcgtgc aaacgaggca caaatgatgg aaggatcgcc
     3781 attcaaggcc cgcttttttg aaccacaccg gcgggcagcg ccccccataa cggcgtatcg
     3841 agcgtaactc ttttacccac aatcgctcaa accgctcccg cgcatatcgt cctcaccatt
     3901 ttggtaggtc gtccattttg cggctgaaac gggttccaac aatccgcgca cgaatcagcc
     3961 gataaagccg ggcgttcctg cgcttcggac ggatttcgtc tccgctggca ccgcgttcga
     4021 tataggactg ccgttgcagg gctcgcactt ggagagtgcg cgaagatcac gtctgtacac
     4081 ttcgcgctgg ttttagaggg tagggtgaaa atgcgcacga caccggattc gtccgcggtt
     4141 cgttcgtcca ggaatcttgc caccgtgccc ggtgaagacc ggcggcggtc ggcgccggca
     4201 gcggttgcga cggagctggc ctccgatacg cggcagctga cgaccaaagc gcgtccggcg
     4261 cgccgccgat cggtgcgatt gccgaacgtg ttgtcgatcg gcatgcccga agggcaaccg
     4321 ctggaaggcg tatcccacat gccggtgcgc cagccgcggg agatcatcga aatgctgcgc
     4381 gtgctgcatt tcaacctcct gctggtcggt ccaaacgcgt tggaaccggc aacatgggcg
     4441 gttttggaac tggtgcaccg atattggccc cagctgcgct gggtgctgat gcccggtgaa
     4501 aacaccggcg accgcgagga aatcatggcc cgctcgcttg gcgcgaccag cgtcggcacc
     4561 gacctgcggg tgatcgttga attggcgggc ggcaagcccg tcgctgtttg aaagtctgcc
     4621 gcgctgagcg agggcgatac gcgccgcagc acgaggttga gcgtcgaaaa agactgggct
     4681 cgtgatctgc ccgaggcgct gcacgtggag acgtgatcgc catcgcggca agcgagcgtg
     4741 tgaacgaaat attgcgggtt gtggccggtc gctcgtggtc gtcggccgtt ctcgtgagat
     4801 gggagagaaa atgaatcgga cttctaagat gggcattctg gctggcggca acgcccggcg
     4861 catgttggcg attgccgcgg cactgactgc gggtctgacc ttggcgtcca ccgcccgggc
     4921 ggcgttgacg ttgacgctct ccgagcccgg cgcgcccggt gggccgatca gcacgaccat
     4981 caccgacaac ggcgccggcg atcaggatct caccaccggc agcatcgaag tcaacgaccc
     5041 gaacttcggc gacttcaaga ccaacattct ggtcggcttc tccaacagga ccgaccccac
     5101 cgccggcccg gtggcggacc tggaagtcca gtcattggac gtgaccaaga acaccaacgt
     5161 ggcggccgcc acgctgatca tcaccttgca ggacaccg
//
LOCUS       DATIMH010000603         1973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1305202, whole genome shotgun sequence.
ACCESSION   DATIMH010000603 DATIMH010000000
VERSION     DATIMH010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305202"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VLI90_10435"
     CDS             <1..380
                     /locus_tag="VLI90_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV14669.1"
                     /translation="SRNVRCTMTGPQPMPVMADGLLIGQAVLNLILNAAEAIEGGGEI
                     SVTFSAPPSESDAKQFHLIVRDSGPGIPSHILDRIFNPFFTTKETGTGLGLAIVHRVV
                     EAHDGTILASNMDGGGARFEIRI"
     gene            442..813
                     /locus_tag="VLI90_10440"
     CDS             442..813
                     /locus_tag="VLI90_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14670.1"
                     /translation="MALLLAAGHGDEPRAAAENSHRTQWEYRVVPEADLAQISDPFTA
                     LATYVEKANKGRSQRWQSGADGGAERKANLPEVQMQQVLIDAALAVMKDKESRLNDFG
                     TQGWELIAIEDHTMIFRRNKN"
     gene            852..>1973
                     /locus_tag="VLI90_10445"
     CDS             852..>1973
                     /locus_tag="VLI90_10445"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSV14671.1"
                     /translation="MARILVVDDQEMMRDSLAGTLVREGHDVVATGDPTVAVERLSAA
                     ARFDLLITDLKMPKMTGIELLAEAKRLRPDLPVVLMTAFATVNTAVEAMKLGAYDYIQ
                     KPFDGDEIKLLVDRTLEHNRLIRENAALRSMTEMNAPRPLVGSSAIMAEVKQRIELVA
                     QSSATVLIRGESGTGKEVVARGIHAASPRRDRPMLAVNCAALSENLLESELFGHEKGA
                     FTGADRLRRGRFELADGGTLLLDEISEIAPALQAKLLRVLQESTFERVGSSVSQQVDV
                     RVIATTNRDLEKCVDDGKFRQDLFYRLNVVPVELPPLRQRGEDIPELCRHFLSLVAKR
                     ENKPFRHMESEAIRVMQRYNWPGNVRELQNIIERASVLET"
BASE COUNT          409 a          563 c          675 g          326 t
ORIGIN      
        1 cgtcgcgcaa cgttcgctgc acgatgactg gtccgcagcc gatgccggtg atggcggatg
       61 gattgctgat cggccaggcg gtgctgaacc tgattctgaa cgccgccgag gcgatcgaag
      121 gcggcggtga gatcagcgtg acgttcagcg cgccgccgag cgaaagcgac gcgaagcagt
      181 ttcacctgat cgtgcgcgac agtggcccgg ggattccatc gcacatcctc gaccgcattt
      241 tcaacccgtt cttcaccacg aaggaaacgg gcacggggct gggattggcg atcgtccatc
      301 gcgtcgtcga agcacacgat ggcacgattc tggcaagcaa catggacggt ggcggcgcgc
      361 ggttcgaaat acggatctaa cgggagagcc gtgatgcatg ccacgaaata attggtgctg
      421 ggcgcgctaa ccgcaacgcc tgtggcgctg ctgctggcgg cggggcacgg cgatgagccg
      481 cgcgcagccg ccgaaaattc gcatcgcacg cagtgggagt accgcgtcgt gcctgaagcg
      541 gacctggcgc aaataagtga tccctttacg gcgctggcca cgtatgtgga gaaggccaac
      601 aagggcaggt cacaacgatg gcaatccggc gccgacggtg gcgctgaacg aaaagccaac
      661 ttgccggaag ttcagatgca gcaggttttg atcgacgcag cgctggccgt gatgaaggac
      721 aaagaatcga ggctgaacga tttcggcacg caaggttggg agctgattgc aatcgaagac
      781 cacacgatga tcttccggcg caacaagaat taatggagtg cggggcgacg cgcggcaagg
      841 gagtgaatgc catggcacga attctggtgg tggacgatca ggagatgatg cgagattccc
      901 tcgcgggcac gctcgtgcgc gaggggcacg acgtcgtcgc caccggtgat cccaccgtgg
      961 cggtcgagcg tctgtcggcg gctgcgcgat tcgatctgct catcaccgac ttgaagatgc
     1021 cgaagatgac cggcatcgag ctgctggccg aggcaaagcg cctgcggccg gacctgccgg
     1081 tggtgctgat gaccgcgttc gccaccgtga acaccgccgt cgaggcgatg aagctggggg
     1141 cgtatgacta catccaaaag ccattcgatg gcgacgagat caagctgctg gtcgatcgaa
     1201 cgctcgagca taatcgcctg atccgcgaga acgcggcgtt gcgcagcatg actgagatga
     1261 acgccccgcg gccgctggtc ggcagcagcg cgatcatggc ggaagtgaag cagcggatcg
     1321 aactggtcgc gcagagcagc gcgacggtgc tgatccgcgg tgaaagcggg accgggaagg
     1381 aagtggtcgc acgcggcatc cacgcggcat cgccgcggcg ggatcggccg atgcttgcgg
     1441 tcaattgcgc cgcgctatcg gagaacttgc tggaatcgga attgttcggc catgagaaag
     1501 gcgcgttcac cggcgccgat cgtcttcgcc gcggccgctt tgaactcgcc gatggtggca
     1561 cgctgctgct tgacgagatc agcgagatcg ccccggcgtt gcaggccaaa ttgcttcgcg
     1621 tactgcagga gagcaccttc gagcgcgtcg gcagcagcgt cagccaacag gttgacgtgc
     1681 gcgtgatcgc gacgacgaac cgcgatctgg aaaagtgcgt tgacgacgga aagttccgcc
     1741 aggatttgtt ctatcggctg aacgtcgtgc cggtcgaact gccgccgctg cggcagcggg
     1801 gggaggatat ccccgagctc tgccggcatt tcctgtcgtt ggtcgccaag cgcgagaaca
     1861 agccgttccg gcacatggaa tccgaagcaa tccgcgtgat gcagcgctac aactggcccg
     1921 gcaacgtgcg cgaactgcag aacatcatcg agcgcgccag cgtgctggaa acg
//
LOCUS       DATIMH010000604         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2454332, whole genome shotgun sequence.
ACCESSION   DATIMH010000604 DATIMH010000000
VERSION     DATIMH010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2454332"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VLI90_10450"
     CDS             complement(<1..435)
                     /locus_tag="VLI90_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14672.1"
                     /translation="MRTNRIAAAVAIMLLVLLFPASGWAAHSFALGTAHGPALNTNKL
                     AGAPAAVIGGRARLIVHVMGSRGGPVAGARVLVRHPRRHSPQFHLRTGGNGVTGASVG
                     NGRWVIRARRPGEGRGRATVVTHSGGVVQVSITLHGARNSFHP"
     gene            complement(447..1316)
                     /locus_tag="VLI90_10455"
     CDS             complement(447..1316)
                     /locus_tag="VLI90_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14673.1"
                     /translation="MTFGGSKRANLSVISAGLIALTVAWAVAKPHQPAAEPAAPTPAA
                     APAGNTPAMELQVDALQKIRDLELTPEQLAVLRKQAATTAATRPSQPAPETTPGYRAA
                     LASLRDALVEGDDEKTSDALDKVERMRDEQLIDTGPDIDSTPAACKAAPDVLSTFSSS
                     QIAEYLAEHADDVPDAWKTMLDALDQSREVKPADYPALRKEAASQVALLMVGLDRAAA
                     EPMERKVGDWLDQAHAMNDDDFKTKRPQVEDDARQIVGHPDAFEQMRHWMQRETADLL
                     SNPELSSALALIK"
     gene            complement(1427..2827)
                     /locus_tag="VLI90_10460"
     CDS             complement(1427..2827)
                     /locus_tag="VLI90_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV14674.1"
                     /translation="MNAIVRTVNLAGTALRRNVMRSALTCLGIIIGVAAVIAMMEIGH
                     GASTAIERSIASMGANTLLVLPGTAASGGVSWGSGSVMTLTPEDCDAILRECPSVRYA
                     APLVRARSSQVVYGNRNWIPQQIMGTTTAYLDIRQWTDMAEGEAFTEQDVRNAGKVCL
                     LGKTLARELFQGVSPIGKDVRIGNVAFRVVGVLKPKGANMMGSDQDDIVLAPWTTIKY
                     RLSNQSMGGLAQVQSSSSSSGGSSSSSSAVNTLSQLYPNVSPELYPPVSEVQAADTPK
                     PVKFANIDLIQVAPSSPSASPAAMKQIQSLLHERHHIREGEPDDFQIRDMGELMSMFS
                     TTTTLMSGLLLGVATISLIVGGVGIMNIMLVSVTERTREIGLRMAVGARARDILRQFL
                     IESIILCLAGGAVGILIGRGASLLLKVLLHLPTQMSVPAIVASVIVSATVGIVFGYYP
                     AWKASRLDPIEALRYE"
     gene            complement(2957..>3145)
                     /locus_tag="VLI90_10465"
     CDS             complement(2957..>3145)
                     /locus_tag="VLI90_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14675.1"
                     /translation="PSAPGNVRPIAVHVGLSDGINTQVSGDGLKEGMTIVVGDATPDD
                     VAGNNSNPFVPQFGKKKS"
BASE COUNT          516 a         1068 c          986 g          575 t
ORIGIN      
        1 gggatggaag ctgttgcgcg cgccgtgcag cgtgatgctg acctgaacca cgccaccgct
       61 gtgggtgacg accgttgccc gccctctgcc ttcaccgggc cggcgggcgc ggatcaccca
      121 gcggccgttg ccgacgctcg cgccggtgac gccattgccg ccggtgcgca ggtggaactg
      181 cggcgagtgc cggcgcggat gtcggaccag cacgcgcgcc cccgcgacag gaccaccgcg
      241 gctgcccatc acgtgcacga tgagccgcgc ccgcccgccg ataactgccg cgggagcgcc
      301 ggcgagtttg ttggtgttga gcgcgggacc gtgcgcggtc cccagcgcga atgagtgagc
      361 cgcccaaccg ctggccggga agagcaagac cagcagcatg attgcaaccg cggcggcaat
      421 tctgttcgtg cgcatcgcca tctcctctac ttgatcaatg ccagggcgct ggacagctcc
      481 gggttggaca acagatcggc cgtctcgcgc tgcatccagt ggcgcatctg ttcgaacgca
      541 tcggggtggc cgacgatctg ccgcgcatcg tcctcgacct gcggccgctt ggtcttgaaa
      601 tcgtcatcgt tcatcgcgtg ggcctgatcg agccagtcac ccacctttcg ttccattggc
      661 tccgccgccg cgcgatccag cccgaccatc aacagcgcca cctgtgatgc ggcctccttg
      721 cgcagcgcgg gatagtccgc cggcttcacc tcgcgcgact ggtccagcgc atcgagcatc
      781 gtcttccacg catccggcac atcatcggca tgctcggcca aatactcggc gatctgcgac
      841 gacgaaaacg tcgagagcac gtcgggcgcc gccttgcacg cggccggcgt ggagtcgatg
      901 tcggggccgg tgtcgatcag ctgctcgtcg cgcatccgct cgaccttgtc cagcgcatcg
      961 gatgttttct cgtcatcgcc ttccaccagc gcatcgcgca gcgacgcaag agccgcgcga
     1021 tatcccggcg tggtctcggg cgcgggttgc gagggcctgg tcgccgcggt cgtggccgct
     1081 tgcttgcgca aaaccgccag ttgctcgggc gtcagctcca gatcgcgaat cttctgcagg
     1141 gcatcgacct gcagctccat ggccggagtg ttccccgccg gcgccgccgc gggcgttggc
     1201 gcggctggct cggctgccgg ttggtgcggc ttggccaccg cccatgccac cgtcagcgcg
     1261 atcaaaccgg ctgatatgac cgagagattg gcacgcttgc ttccaccaaa agtcatctcg
     1321 aacctcgatg tagtagagga ccgaccctaa cctcccgccg atgtgagaac ggcggtgcga
     1381 ctattttatt gcgattgtga tccccacgcg gggttcatca atctttctat tcgtaccgca
     1441 gcgcctcgat cggatcgagc cgcgaggctt tccacgcggg gtagtaaccg aagacgatgc
     1501 ccacggtggc cgagacgatg accgatgcga cgatggccgg caccgacatc tgcgtgggca
     1561 ggtgcagcag caccttcagc agcagcgatg cgccgcggcc gatcaggatg ccgacggcgc
     1621 caccggccag gcagaggatg atcgactcga tcaggaactg tcgcaggatg tcgcgggcgc
     1681 gcgcgccgac cgccatgcgc aggccgatct cgcgcgtgcg ctcggtcacg ctgaccagca
     1741 tgatgttcat gatgccgacc ccgccgacga tcagcgagat cgtcgccacg cccagcagca
     1801 ggccggacat cagcgtggtc gtcgtgctga acatggacat caattcgccc atgtcgcgga
     1861 tctgaaaatc gtccggctca ccctcgcgga tgtgatgccg ctcgtgcagc agcgattgga
     1921 tctgcttcat cgccgccggc gatgccgatg gactgctggg ggcgacctgg atcaaatcga
     1981 tgttcgcgaa ctttactggc ttgggtgtgt cggccgcctg tacttccgac accggcgggt
     2041 acaactccgg cgagacgttg ggatacagct ggctgagcgt gttgacggcc gagctggacg
     2101 aactgctgcc gccgcttgaa cttgatgagg attgcacctg ggccagaccg cccatcgact
     2161 ggttcgacag ccggtatttg atcgtcgtcc acggcgccag cacgatgtcg tcctggtcgc
     2221 tgcccatcat gttcgcgccc ttgggcttca gcacgccgac cacgcggaac gcgacgttgc
     2281 cgatgcgcac gtccttgccg atcggcgaca cgccctggaa cagctcgcgc gccagcgtct
     2341 tgcccagcag gcaaactttt ccggcgttgc gcacgtcctg ctcggtgaat gcttcgccct
     2401 cggccatgtc ggtccactgg cggatgtcga ggtacgcggt cgtggtcccc atgatctgct
     2461 gcgggatcca attgcggtta ccgtagacga cttgcgacga tcgcgcccgg accagcgggg
     2521 ccgcgtagcg caccgacggg cattcgcgca ggatggcgtc gcaatcttcc ggcgtcagcg
     2581 tcatcacgct gcccgatccc cacgacaccc caccgctggc cgccgtcccc ggcagcacca
     2641 gcagcgtgtt ggcgcccatg ctggcgatgc tgcgctcgat cgcggtcgat gccccgtgac
     2701 cgatctccat catcgcgata acggcggcca cgccgatgat gatgccgagg cacgtgagcg
     2761 ccgaacgcat cacgttccgg cgaagcgctg tgccggcgag gttgacggtc ctgacgatgg
     2821 cgttcatttg aaaatcatct cggcatcaaa ggattcacga ttctccaacc gccttttagt
     2881 tgcggcccgg agggccgagc gttcggtgca gtcagaacct tcgacaatcg cgatccgtca
     2941 ttcacccggc gcgcgatcaa ctcttcttct tcccaaactg cggcacaaac gggttcgaat
     3001 tattccccgc gacatcatcc ggcgtcgcgt cgccgacgac gatcgtcatc ccttctttca
     3061 acccgtcgcc actcacctgc gtgttgatgc catcactaag gcccacgtgc acggcgatcg
     3121 gtctgacgtt gccgggagcg ctggg
//
LOCUS       DATIMH010000605         1954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5634005, whole genome shotgun sequence.
ACCESSION   DATIMH010000605 DATIMH010000000
VERSION     DATIMH010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5634005"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1921)
                     /locus_tag="VLI90_10470"
     CDS             complement(<1..1921)
                     /locus_tag="VLI90_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HSV14676.1"
                     /translation="MFAHAALQFKSVSGVSDMRKSPLILTVFVLAAALAPRASAQCVS
                     LTTLGSAYTQNFDTLSNVAGSTTNNLTLPGWFMTETGGGARDNEQYGVDTGGSTTGDT
                     YSYGAAGSTERALGQLRSGTLIPNFGACFTNNTGSAITSLAVAYNGEQWRLGTAGRTD
                     QMNFEYSLNSTDLVTGTWINVAALNFVTPDTVTAGAKNGNAAADRTALSSTINSLSIP
                     NGATFWIRWNDQDATGADDGLAVDDFSLTPQGGAISLSGVGSATPNPVTAGNATTLSV
                     LVTPGSTSTGIVVTCNLSSIGGSSSFSLGTVNGNTYSAQYTVPVATVANTYPLACTVT
                     DAQSHSANAPISLAVASAAPTNPTGVGSAAPNSVQAGAFTLLTVTVTAGANPVSTGIT
                     VTADLSAIGGSATQQFFNDGTNGDVTANDSIFSFNATVASGASPGSKSLPATINDVQA
                     RSGAASIALTVQAPAPTTIKISQVYGGGGNSGATYSNDFVELFNQSTTAVDVSGWSVQ
                     KASGTQANWEITNLCPSGGTCVIQPGHYYLVQEAAGTNVNTVALPAADVTGIIAMGAG
                     SGKIALVANQTLLTGNCPTGLGIVDFVGYGGSGSANCSEGTPAGTPSATAAAIRKRNG
                     CVDTDNNANDLIIGGP"
BASE COUNT          318 a          520 c          740 g          376 t
ORIGIN      
        1 tgggtccgcc aatgatcagg tcgttggcat tgttgtcggt atcgacgcaa ccattgcgct
       61 tgcggattgc cgcggcggtg gcgctgggcg ttccggccgg tgtgccttcg gagcaatttg
      121 ccgaaccact gccgccatag ccgacgaaat ccacgatgcc gagaccggtg gggcaattgc
      181 cggtgagtag cgtctgattg gcgaccaggg cgatcttgcc cgagccggcg cccatggcga
      241 tgatgcccgt gacgtcggcg gcgggaagcg cgaccgtatt aacattcgtg ccggcagcct
      301 cctgcaccag gtagtagtgc ccgggctgga tcacgcaggt tccgccgcta gggcagagat
      361 tggtgatttc ccagttcgcc tgagtgcccg aggccttttg cacgctccag ccgctgacgt
      421 caaccgcagt ggtggactgg ttgaagagtt cgacgaagtc gttggaataa gtcgcacccg
      481 agttgccgcc gccgccgtac acctggctga tcttgatggt ggtcggggcg ggagcctgta
      541 cggtaagcgc gatcgaggcg gcgccgctgc gcgcctggac gtcgttgatc gtggccggca
      601 gggacttgct gccgggcgag gccccgctcg ctacggtggc attgaacgag aagatgctgt
      661 cgttggcggt gacgtcgccg ttcgtgccgt cgttgaagaa ctgctgggtg gcggagccgc
      721 cgatggcgga caggtctgcc gtgacggtga tcccggtgct gaccgggttt gccccggcgg
      781 tgacggtgac ggtcagaagg gtgaacgccc cggcctgtac gctgttcgga gccgcggagc
      841 cgacgcctgt gggattggtg ggcgcggcgg atgcgaccgc tagagagatg ggcgcatttg
      901 ccgagtggct ctgcgcgtcg gttaccgtgc aagcgagcgg gtaggtgttg gcgactgtgg
      961 ctacgggcac cgtgtattgg gcggaatagg tattgccgtt gaccgtgccc aggctgaacg
     1021 aggaactgcc gccgatcgat gagagattgc aggtcacgac gatgcctgtg gaagtggagc
     1081 ccggtgtgac gaggacggag agggtggtgg cattgccggc cgtgacgggg ttcggagtgg
     1141 ccgagcccac gccggaaagg gagatggctc cgccttgcgg cgtgagcgag aaatcgtcga
     1201 ccgcgaggcc gtcgtcggcg cccgtggcgt cctggtcgtt ccagcgaatc cagaaggtgg
     1261 cgccgttggg aatgctgagc gagttaatcg tgctggaaag ggccgtgcga tcggcagcgg
     1321 cgttgccgtt tttggcgccg gcggtgacgg tatccggggt gacgaaattg agcgcggcga
     1381 cgttgatcca ggttccggtg acgaggtccg tggagttgag gctgtactcg aaattcatct
     1441 ggtcggtgcg gccggcggtg ccgaggcgcc actgttcgcc gttatacgcg acggctaagg
     1501 acgtgatggc ggagccggtg ttattcgtga agcacgcgcc gaagttggga atgagggtgc
     1561 cgctgcgcag ttggccaagc gcgcgttcgg tggagcccgc ggcgccatag ctgtacgtgt
     1621 cgcccgtggt gctgccgccg gtatcgacgc cgtactgctc gttatcgcgc gcgccgccgc
     1681 cggtttcggt catgaaccag ccgggaagag tcagattgtt tgtggtggaa cctgcgacgt
     1741 tggagagcgt gtcgaagttc tgggtgtaag cggagcccag ggtagttagg gagacgcatt
     1801 gtgcggaggc gcgcggagcc agtgcggcgg ccagcacaaa cacggtcaaa atgagcggtg
     1861 atttacgcat atcgctaact cccgagacag atttgaattg cagcgcggcg tgggcgaaca
     1921 tgtggccccc ctgaagatcg gattggtgtg caca
//
LOCUS       DATIMH010000606         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1883018, whole genome shotgun sequence.
ACCESSION   DATIMH010000606 DATIMH010000000
VERSION     DATIMH010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883018"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VLI90_10475"
     CDS             <1..396
                     /locus_tag="VLI90_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSV14677.1"
                     /translation="ILSQLHAIGVRISLDDFGTGYSSLSYLHNFPMQKVKIDRSFLEG
                     IDSDRPLTLLRGVARLSADLGMSVAVEGIETNEQLELISADGAVTEAQGYLFSPPVPA
                     VRVRQLLNASHGRRPPEGMLVSVASRSIA"
     gene            587..1273
                     /locus_tag="VLI90_10480"
     CDS             587..1273
                     /locus_tag="VLI90_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14678.1"
                     /translation="MDQVDYRLAQTPEEKEEIYNLRYRAYLREGAVKESPAQRVTDQY
                     DDLPNSWTFGVYLMEELYSSVRISVLTSEWRESCSAEAFGEILYPRLDRGEVIIDPAR
                     FVADPDKVKRFPELPYLTLRLAYMACEYFNADLGLAIVRAEHQGFYRRVFLHETIAEP
                     RLFPGLLKPVGLMAADFPTMKEKVFERYPMMRSTAFERRMLFERPGSRTSRPARVASG
                     HQRAQAVPTS"
     gene            1610..2101
                     /gene="pal"
                     /locus_tag="VLI90_10485"
     CDS             1610..2101
                     /gene="pal"
                     /locus_tag="VLI90_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HSV14679.1"
                     /translation="MKYQMRILQGWKLAAVVAVALSMGACAKNNIGADGAMASAATPG
                     SQQDFVVNVGDRVFFESDQTELSPQAIATLEKQAQWLQTYNRYSFTIEGHADERGTRE
                     YNIALGARRAQSVRSYLASRGIDANRMRTISYGKERPVAVCNDISCWSQNRRAVTVLN
                     AGA"
BASE COUNT          418 a          709 c          674 g          451 t
ORIGIN      
        1 atcctgtcgc aattgcatgc gatcggcgtg cgcatttcgc tggatgattt cggcaccggc
       61 tattcgagcc tcagctacct gcacaatttc ccgatgcaga aggtgaagat cgaccgctcc
      121 ttcctcgaag gcatcgacag cgaccgtccg ctgacgctgc tgcgcggcgt ggcgcggctg
      181 tcggccgacc tcggaatgtc ggtcgcggtg gagggcatcg agaccaacga gcagctcgaa
      241 ctgatcagcg ccgacggcgc ggtgaccgag gcgcagggtt atctgttcag cccgccggtg
      301 cccgccgtgc gcgttcgcca gttgctcaac gcctcgcacg gccgccgtcc gccggagggc
      361 atgctggtgt cggtcgcctc gcgatccatc gcctgattcc cgcgtcccgg aaaaatgctc
      421 gtatccggat ttatacgggg taaccataaa ccattgaggg ctttgcaatc tcgttaacaa
      481 gctgttaatc gtctactcgc tcgtagaagt tgcttgttag cgttaggagt acagcatgag
      541 gtccccggcc gaagttgcga cggcctttgg gcggagttcg gacctgttgg atcaagtcga
      601 ctatcgtctg gcgcaaacac ccgaagaaaa agaagagatc tacaacctcc gctatcgtgc
      661 ctatcttcgc gaaggcgcgg tgaaggagtc tcccgcacag cgcgtcactg accaatacga
      721 cgacctgccg aattcctgga cgtttggcgt ctatctgatg gaagagctct atagctccgt
      781 tcgcatcagc gttctgacct cggagtggcg ggagtcctgt tcggcggaag cgttcggcga
      841 aatcctgtat ccccggctcg atcggggcga ggtcatcatc gatcccgcgc gcttcgtcgc
      901 cgatcccgac aaggtcaagc ggtttcccga actgccctac ctgacgctgc ggctcgccta
      961 catggcgtgc gaatatttca acgccgatct tgggctcgcg atcgtgcgcg ccgagcatca
     1021 ggggttttat cgccgcgtat tcctgcacga gaccattgcc gaaccccgcc tgtttcccgg
     1081 cctgctcaag ccggtcgggc tgatggcggc ggatttcccg acgatgaagg aaaaggtctt
     1141 cgagcgctat ccgatgatgc gatcgaccgc tttcgagcgt cggatgctgt ttgagcgccc
     1201 cggcagccgg accagccggc cggctcgcgt cgcctccggc catcagcgtg cccaggccgt
     1261 cccgacgtcc tgaggcccac cggatccacc ctgagaatca atcccgcgcg ccgtaccggt
     1321 ctgccgccgc tcgcttgagg ttttggaggc gtgtcgggct tcccgggacc ggcgtatgtc
     1381 gtgccgacag tgcgcgatac cccctaaatc cggtggttta ggcccgatgc tcgcttgcct
     1441 tcaccgtcgc gccacattta caaatcatta actatcgcgg gcgcttttca cggtttgtcg
     1501 gcatttgata gtgtcccgca aggttccatc aaggttgacg gaacgttcgc ttaaccatcg
     1561 acctgtagac cggttagcag tcgaaaaacg tgagcgtgga ggctccggaa tgaaatatca
     1621 gatgcgtatc ctccagggat ggaagctggc ggctgtggtc gcggtggcgc tgtcgatggg
     1681 cgcctgcgcc aagaacaaca tcggcgccga cggcgcgatg gcgagcgcgg caaccccggg
     1741 gagccagcag gatttcgtgg tcaatgtcgg cgaccgcgtg ttcttcgaaa gcgaccagac
     1801 cgagctgagc ccgcaggcga tcgcgacgct cgagaagcag gcgcagtggc tgcagaccta
     1861 caaccgctac tccttcacca tcgaaggcca tgccgacgag cgcggtaccc gcgaatacaa
     1921 catcgcgctt ggcgcccgcc gcgcccagtc ggtgcgcagc tatctcgcct cgcgcggcat
     1981 cgatgcgaac cgcatgcgca ccatctccta cggcaaggag cgtccggtgg cggtctgcaa
     2041 cgacatctcc tgctggtcgc agaaccgccg cgccgtcacg gtgctgaacg ccggcgcctg
     2101 atctttcagc ccttcgcaga gctcaaaaaa ccggcgtctt cagacgccgg tttaactgcg
     2161 ctttatgctt gcaatccaac tggatgcacg cgtttgttaa cgaagctcac caacaagtta
     2221 atctagcgtc gcaggatgaa ttcccgcagc ag
//
LOCUS       DATIMH010000607         3734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_727126, whole genome shotgun sequence.
ACCESSION   DATIMH010000607 DATIMH010000000
VERSION     DATIMH010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727126"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VLI90_10490"
     CDS             complement(<1..922)
                     /locus_tag="VLI90_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV14680.1"
                     /translation="MNLVALKMLIGDRLKYIALVAGVAFAALLITQQASIFTGYTLEI
                     GAWIRDTGAVDLWVSDPQMEHVEDFKPLTDTALNRVRSVSGVAWAVPIYKGYLKSRLP
                     DGTLVNARVIGLDDATLAGGPPEMASGHLSDLRGDDAVIVNEEDLNGQLALHRGGENR
                     PLRVGDHISLNDHDARVVGTFRATHEFFWQPVVYTTYSQALTWAPQERKLLTYVLVKV
                     RPDESVSAVAERIHQQTRLLARTTHEFDWDTTAWVLKKTGILVNFGITIALGFVIGVL
                     IAGQTFYMFILDNLKYFAALKAMGAGTLTLV"
     gene            complement(919..1590)
                     /locus_tag="VLI90_10495"
     CDS             complement(919..1590)
                     /locus_tag="VLI90_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSV14681.1"
                     /translation="MGLKERRERERSELRTRIMDAARQLFLREGYDAVSMRRIADAIE
                     YSPTAIYVYFKDKESLIREMCRVDFGNLAGAVGNLAKIADPIERIRQLGVAYIRFAVQ
                     KPNHYRLMFMTTFSGIGPTAADIERKNNPDQDAYAFLIQAVREGLAAGRFRPELTSAE
                     LIAQVLWSAVHGVASLHIVKANDPWLEWAPLEQRTNLAVDAILRGLVREDSGKKTAPA
                     KGKKR"
     gene            complement(1660..2817)
                     /locus_tag="VLI90_10500"
     CDS             complement(1660..2817)
                     /locus_tag="VLI90_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV14682.1"
                     /translation="MNFVAIKMLVGDRAKYLGIIMGLTFASLLITQQLAIFCGLMARS
                     YGGISDMAYPDIWVMDSKVQFIDDIKPLQDTELYRVRGVAGVEWAVPLYKGLIKARLS
                     NGNFQTCIVYGLDDASLVGGPPYIVDGKLSDLRRSEGVIVDEVGARDKLAHVAPDGVT
                     KIPLKVGDEMELNDHRAVVVGICRNTRTWQSQPVVYTTYTRATTFAPQERKLLSFVLV
                     KAVGGVDQVKLCQRITRATGLAALTRDGFIEKTYYYFMKYTGIPINFGISVALGFIVG
                     TAIAGQTFYSFTLENIKHFGALKAMGAGNAMLLRMILLQAILTGVIGYGLGVGLATCF
                     GFFTQNSELAFKLPGWLLGFTALAVTMICIASALLSVRKVMKLEPAIVFKG"
     gene            complement(2814..3530)
                     /locus_tag="VLI90_10505"
     CDS             complement(2814..3530)
                     /locus_tag="VLI90_10505"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HSV14683.1"
                     /translation="MNEIKTRVNDADDRRTHRRLLEAAGEVFAAKGYRATTVREICRR
                     AGANVAAINYHFGDKEGLYAEVLRYAHACAMERHPPEMHTTAASSPEDRLRAFIVSFL
                     HRLFDQGRPAWHARLMIREMVEPTGALGRLVKQSLHPRREQLTQIIRAIIGDHRADDR
                     LLRHCVMSVVGQCLSFYHGRPMLERLYPEQKFDAAGLAERADYIYRFTLAALRGIGTV
                     ETSKKARLPLKRQARSEVRA"
     gene            complement(3543..>3734)
                     /locus_tag="VLI90_10510"
     CDS             complement(3543..>3734)
                     /locus_tag="VLI90_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14684.1"
                     /translation="AQGHEQRDAFGAGDVSESTHVERDCIAGAGINHLRINAALTGDS
                     AKGAARSIRCGFDHFKHPF"
BASE COUNT          703 a         1242 c         1070 g          719 t
ORIGIN      
        1 gcaccagcgt cagcgtgccg gcgcccatcg ccttgagcgc ggcgaagtat ttcaggttgt
       61 cgaggatgaa catgtagaac gtctgcccag cgatcagcac gccgatcaca aaccccagcg
      121 cgatcgtgat gccgaagttg acgaggatgc cggtcttctt cagcacccat gcggtggtgt
      181 cccagtcgaa ctcgtgcgtc gtgcgggcga gcaatctcgt ctgttgatga atgcgctccg
      241 ccaccgcgct cacgctttca tcggggcgaa ctttcaccag cacgtacgtc agcagctttc
      301 gttcctgcgg cgcccacgtc agcgcctggc tgtatgtggt gtagacgacc ggctgccaga
      361 aaaattcgtg cgtcgcccga aacgtgccga cgacgcgggc gtcgtgatcg ttcagtgaaa
      421 tgtgatcgcc aacgcgcagc gggcggttct caccgccgcg gtgtaacgcc agctggccgt
      481 tcagatcctc ttcgttgacg atgacggcat cgtcgccgcg caggtcgctc agatgaccgc
      541 tggccatctc cggtggccca ccggccagag tcgcatcgtc cagcccgatc acccgcgcgt
      601 tgaccagcgt cccatccggc aggcgcgact tgagatatcc cttgtaaatc ggcaccgccc
      661 aggcgacgcc gctgacgctg cggactcgat tgagcgccgt gtcggtgagc ggcttgaaat
      721 cttccacgtg ctccatctgc ggatcggata cccacagatc gaccgcgccg gtgtcgcgaa
      781 tccacgcgcc gatctccaac gtgtaacccg tgaagatcga cgcctgctgc gtgattaata
      841 gcgccgcgaa cgcgacgccc gcgaccagcg caatgtattt gagacgatcg ccgatgagca
      901 ttttcagcgc aacgaggttc atcgcttctt tcccttcgcc ggtgctgttt ttttgccgct
      961 atcttcgcgg acgagaccgc ggaggatggc gtcaaccgcc aggttcgtgc gctgctccag
     1021 cggcgcccac tcaagccagg gatcattcgc tttaacaatg tgcagcgatg ccacgccgtg
     1081 caccgccgac cagagcacct gcgcgatcag ttcagcgctg gtcagttccg gccgaaaccg
     1141 ccccgccgcc aggccttcgc gtaccgcctg gatcaaaaac gcgtaggcgt cctggtcggg
     1201 attgttcttg cgctcgatgt ccgccgcggt cggaccgatg ccggagaacg tcgtcatgaa
     1261 catcagccga tagtggttcg gcttctgcac cgcgaagcga atgtacgcga cgcccagctg
     1321 gcggatgcgc tcgatcggat ctgcgatctt cgccaggttc cccaccgcgc cggccaggtt
     1381 gccaaaatcc acccggcaca tttcgcggat gagcgattcc ttgtccttga agtagacgta
     1441 gatcgcggtg ggggaatact cgatggcgtc ggcgatccgc cgcatggaga cggcgtcata
     1501 tccttcgcgc agaaacaact gccgggcggc atccatgatg cgcgtccgca attcgctgcg
     1561 ctcgcgttct cgtcgttcct tcaatcccat gacggcggaa caatagttaa cggcgttaag
     1621 taagtcaaca ctgttttacg gattgctgca actccaggct cagcctttaa atacgatcgc
     1681 gggctccaac ttcatcacct tgcgaacgct gagcagcgcg ctggcgatgc agatcatcgt
     1741 caccgccaga gcggtgaagc cgagcaacca accgggcagc ttgaacgcca gttcgctgtt
     1801 ctgcgtgaag aacccgaagc acgtcgccag accgacgccc aggccgtagc cgatcacgcc
     1861 ggtcaggatg gcctgcagca ggatcatgcg cagcagcatc gcgttaccag cgcccatcgc
     1921 cttgagcgcg ccgaaatgtt taatgttctc cagcgtgaag ctgtagaacg tctgccccgc
     1981 gatggcggtg ccgacgatga accccagcgc caccgaaatg ccgaagttga tcgggatgcc
     2041 ggtgtatttc ataaagtagt agtaggtctt ctcgatgaac ccatcgcgcg tcaacgcggc
     2101 cagcccggtg gcgcgcgtga tgcgctggca caacttcacc tgatccacgc ctcccaccgc
     2161 tttcaccagc acgaacgaca gcagcttgcg ctcctgcggc gcgaacgtcg tcgcccgcgt
     2221 gtatgttgtg taaacgacgg gttggctctg ccacgtgcgc gtgttgcggc agatgccgac
     2281 caccaccgcg cggtgatcgt ttaactccat ctcatcgccc accttcagcg ggatcttggt
     2341 cacgccatcg ggcgcaacgt gcgcgagctt gtcgcgcgcg cccacttcat cgacgatgac
     2401 accttcactt cgccgcaggt ccgacagctt tccgtcgacg atgtaaggcg gaccaccgac
     2461 gaggctcgca tcgtcgagcc cgtacacgat gcacgtctga aagttcccgt tgctgagccg
     2521 ggctttgatc agccccttgt aaagcggcac cgcccactcg acgccggcca cgccacggac
     2581 gcgatacagc tcggtgtcct gcagcggttt gatgtcgtcg atgaactgaa ccttggagtc
     2641 catgacccag atgtccggat acgccatgtc gctgatgccg ccgtaggaac gcgccatcaa
     2701 cccgcagaag atcgccagct gctgcgtgat cagcagcgag gcgaacgtca gccccatgat
     2761 aatgccgaga tatttcgccc ggtcgccgac aagcattttg atcgcgacga aattcatgcc
     2821 cgcacctccg accgggcttg ccgcttcagc ggtaagcgtg ccttcttcga ggtctcaacg
     2881 gtcccaatcc ccctgagcgc cgccaacgtg aagcgataga tgtaatccgc gcgctccgcg
     2941 agccccgcgg catcgaattt ctgctcggga taaagccgtt ccagcatcgg ccggccgtga
     3001 taaaacgaca gacattgccc gacgacgctc atcacgcaat gccgcagcaa tcgatcgtct
     3061 gcgcgatggt cgccgatgat cgcgcggatg atctgcgtca gctgctcccg ccgcgggtgc
     3121 aggctctgct tcaccagccg gccgagcgcg ccagtgggtt ccaccatctc gcggatcatc
     3181 aaccgcgcgt gccacgcggg ccggccctgg tcgaacaacc ggtgtagaaa tgagacgatg
     3241 aacgcccgca atcgatcctc cggcgacgac gcggccgtcg tgtgcatctc cggtggatgc
     3301 cgctccatcg cgcaagcatg cgcgtagcgc aacacctcgg cgtacagccc ctccttgtcg
     3361 ccgaagtggt aattgatcgc cgccacgttc gccccggcgc gtcggcagat ctcgcgcacg
     3421 gtcgtcgcgc gatatccctt ggctgcgaac acttcccccg ccgcctccag caaccggcgg
     3481 tgcgtgcgcc ggtcgtctgc gtcgttaact cttgtcttaa tctcgttcat tcaatcgtaa
     3541 gcttaaaacg gatgtttgaa atggtcaaac ccgcacctga tggatcgagc tgcgcccttc
     3601 gcggaatcgc cggttaacgc agcattaatg cgcagatgat taatccccgc ccccgcaata
     3661 caatctcgct cgacatgcgt cgattccgac acatcccccg cgccgaaggc atcgcgctgc
     3721 tcgtgtccct gggc
//
LOCUS       DATIMH010000608         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5349098, whole genome shotgun sequence.
ACCESSION   DATIMH010000608 DATIMH010000000
VERSION     DATIMH010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5349098"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1998)
                     /locus_tag="VLI90_10515"
     CDS             complement(<1..1998)
                     /locus_tag="VLI90_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14685.1"
                     /translation="MLQYHNDLRSTGQNLNETAITYATLNASQFAKQFATPVDGQVFA
                     QPLYMPQLSIVGGPQPGVHDVVFAATEHGSVYAIDAAGGNVLWRTSLIDVANPRVNLL
                     GATTIAPVPSDETHSGDLTPEISITATPVIDPAAGALFVAAKTKQSIAGADHYAQTLF
                     KLDIHSGAVVAGSTFADTKMQGGRFTYRYGDDPFVRGTGAGAVKDPKDTAGESRVYFN
                     ALREFGRSALVLDHGRLTECFASHGDNGPYHGWVLQFDPTSLAVVAALNVSPNGDGDG
                     IWQSGAPPAVDERGDLFLEVGNGTFDGNADAAGKTIGLDDAGFPKDGDYGDCFLRITI
                     DPDHPSNDNQNRNGWGLKVADYFSPFNNHALDRRDTDVGSGGVLLLPDAVGADAHRHL
                     LVGAGKEGKIYLLDRDDLGKFDPATDHAVQTLGRSRDSGINASFGVPALFNGSIYYFP
                     GYGGDGRVFHLENGGATLAATCTSRTADRFGALIGTPSISADGRVNGVVWILDRGSNQ
                     LRAYRADDLNVRLWSSGDAPHDRDRLGMVTKFSVPTIADGRAFVGTADHLVVYGPPRP
                     PTSAPPPPSAVVAEARAFNLVAIHWQDNASNEDGFHVERSTDGSTWTTVATVSSNETT
                     ATDPSTLAQTAYEYRVSAFNGFNGGSSATSTEESKVTTPAAP"
BASE COUNT          406 a          750 c          798 g          431 t
ORIGIN      
        1 cggggcggcg ggggtggtga ctttggactc ttcagtcgag gtcgcgctcg atccgccgtt
       61 gaaaccgttg aacgcgctga cgcgatattc atacgcggtc tgcgccagcg tcgatggatc
      121 ggtggcggtc gtttcgttgg agctgacggt ggcgacggtc gtccacgtcg agccatcggt
      181 ggatcgctcg acgtgaaatc cgtcttcgtt gctggcgttg tcctgccagt gaatcgcgac
      241 gaggttgaac gcccgcgcct cggcgacgac cgccgacggc ggcggtgggg cgctggtggg
      301 cggcctgggt gggccgtaga cgaccaggtg atcggcggtg ccgacgaacg cccggccgtc
      361 ggcgatcgtc ggcaccgaga acttcgtcac catgcccagc cggtcgcggt cgtgcggcgc
      421 gtcaccgctg ctccacaggc gcacgttcaa gtcatcagcg cgatacgcgc gcagctggtt
      481 cgacccgcgg tccagtatcc acacgacgcc gttgacgcgg ccgtcggccg agatcgatgg
      541 tgttccgatg agtgcgccaa atcgatcggc cgtgcgcgag gtgcacgttg ccgccagcgt
      601 ggcgccgccg ttttccaggt ggaacacccg gccgtcaccg ccgtaaccgg ggaagtagta
      661 gatcgaaccg ttgaagagcg ccggcacgcc aaacgacgcg ttgatcccgc tgtcgcgcga
      721 tcgacccagc gtctgcaccg cgtgatcggt cgccggatcg aacttaccaa gatcatcgcg
      781 atccagcagg taaatcttgc cttccttgcc cgcgccgacg agcaggtggc gatgggcgtc
      841 tgcgcccacc gcgtccggca gcagcagtac gccaccggaa ccgacgtcgg tgtcgcgacg
      901 atccagcgcg tggttgttga acggcgaaaa ataatcggcc accttcagac cccagccgtt
      961 gcggttctgg ttgtcgttcg acggatggtc cggatcgatc gtgatgcgca gaaagcaatc
     1021 gccgtaatcg ccgtccttgg gaaaacccgc gtcgtcgagg ccgatcgtct tgccggcggc
     1081 gtcggcgttg ccgtcgaacg tgccgttgcc gacctcgagg aacagatcgc cgcgctcgtc
     1141 cacggcgggc ggcgcgccgc tttgccagat gccgtcgccg tcgccgttgg gcgaaacgtt
     1201 cagcgccgca acgaccgcga gcgatgtcgg atcgaactgc agcacccagc cgtggtatgg
     1261 tccgttgtcg ccgtgcgatg cgaagcactc ggtaaggcgg ccgtgatcga gcaccagcgc
     1321 cgagcggccg aactcgcgca gagcattgaa gtacacccgg ctttcgccgg cggtgtcctt
     1381 tggatctttc accgcgcccg cgccggtgcc gcggacgaaa ggatcatcgc cgtagcgata
     1441 cgtgaaccgt ccgccctgca tcttggtgtc agcgaatgtt gaaccggcga cgaccgcgcc
     1501 gctgtggatg tccagcttga acaacgtctg cgcgtaatga tcggcgcctg cgatcgattg
     1561 cttcgtcttg gcggcgacga acagcgcgcc cgccgccggg tcgatgaccg gggtggcggt
     1621 gatgctgatt tccggtgtga ggtcgccgct gtgcgtttca tcactgggga ctggcgcgat
     1681 ggtcgtcgcg ccaagcaagt tcacgcgtgg gttagcgacg tcgatcaacg acgtccgcca
     1741 aagcacgttg ccccccgcgg cgtcgatggc gtagaccgag ccgtgctcgg tcgcggcgaa
     1801 cacgacatcg tgcacgcccg gctgcgggcc gccgacgatc gacagctgcg gcatatacag
     1861 cggctgcgcg aatacctgcc catccacggg cgtcgcgaac tgcttcgcga actgagaggc
     1921 gttcaacgtc gcgtaggtaa tggcggtttc gtttaggttc tgaccggtgc tgcgcaggtc
     1981 gttgtgatat tgcagcacgc tgactcgatg cggtggatcc gcggcgggcg cgagcgatgc
     2041 gaagatcgcg acaacgaaga gtgatgtaat agcccaggcc gaaaggccgg gggtcgtgcg
     2101 ctcaacggcg ccgcttgcag ttcgatcccc ggacttcggg cctgcgctat gaagggcacg
     2161 gattgttgat aaccaccgac tgaattgcaa aactcttccc gcgtttcgat tcacgtcaac
     2221 tcctccagca cgcgcacgaa gtctacggcg acgacggcgc gaaatgaaga tatccgtgcg
     2281 caacaatact cgcagttcgc cattcgactg ccgtcccgcg gcagcgccgc ggggttttga
     2341 gcgtaacggc cagaatcgag ctgagtcatc cccactttgg gacca
//
LOCUS       DATIMH010000609         3065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_147088, whole genome shotgun sequence.
ACCESSION   DATIMH010000609 DATIMH010000000
VERSION     DATIMH010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147088"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VLI90_10520"
     CDS             complement(<1..362)
                     /locus_tag="VLI90_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14686.1"
                     /translation="MDSFLIRGGSRLKGKIEISGSKNSALPIVAACLLAEGKTTLHSV
                     PRLSDIDSMNKLIGELGCHVFRHEPKTKTVGDGPDLNGPLDIEVQDEKLCEARYDIVK
                     TMRASICVLGPLLAKRGKA"
     gene            complement(706..1539)
                     /locus_tag="VLI90_10525"
     CDS             complement(706..1539)
                     /locus_tag="VLI90_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HSV14687.1"
                     /translation="MKERKPPNDKQEAKASDKDVTPVLRDWDYEAGTINVRKVSGLDG
                     RPKLQMRLDLGLLQMEVTGRPDGDRPFGRESLLEHHELELQEYKRKHGSEDGFQLTGD
                     ECQSLREEAVMYYHRYLSLFVLEDFTGVVRDTARNLRVLDLCSQFAVEEQDRLILEQY
                     RPYITMMNARAQASIHVQGKEFDAAIKTVEAGLARIHEFYEQFGQEEAFARSNEVRVL
                     KRFSREIRKKLPVDPMQQLRRRLDRAVKDERYEDAAKLRDEITARQGAVSHHSNSKTV
                     K"
     gene            1754..1921
                     /locus_tag="VLI90_10530"
     CDS             1754..1921
                     /locus_tag="VLI90_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallothionein"
                     /protein_id="HSV14688.1"
                     /translation="MGDESAKCACPSCNCRVPPGKGVVRDGKAYCSSVCAYECTPTTC
                     LCVHDRCERGK"
     gene            2068..>3065
                     /locus_tag="VLI90_10535"
     CDS             2068..>3065
                     /locus_tag="VLI90_10535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV14689.1"
                     /translation="MRICLYTGTALPKLGGQEAVVDALARQFQKLGHEPFVMAPRPRL
                     PLRPNDASLPYPVIRHPRFYSTKFLVSWYRRWLRAAYLRHHFDVIHCHDVYPTGYLAA
                     LTNNDLRIPIVITSHGGDVREGNVRLSKPGMRPRFTRAVSDADALVSIGRFTEEGFRR
                     LGANATKIVTIPNGVDLDPFREPAARPADLDARIVPAEYALFLGRLALRKGVDTLLQA
                     LAQVTPNGRVQLVIAGSGDERPAIEQMIDKLNLRDRVRLVGRVVGDLKTYLLQNALCT
                     VMPSRGWEAFPLVVLESFAAGRPVIGSRIPGLEDLIAPDVTGLLFTDESAADLAGAL"
BASE COUNT          510 a          969 c          952 g          634 t
ORIGIN      
        1 gctttgccgc gcttcgccag cagcgggccg agcacgcaga tgctggcgcg catcgtcttg
       61 acgatgtcgt agcgcgcttc gcatagcttc tcgtcctgca cttcgatgtc gagcgggccg
      121 ttcagatccg ggccatcacc gacggtcttg gtcttgggtt catggcgaaa gacgtgacag
      181 cccagctcgc cgatcagctt gttcatcgaa tcgatgtcgc tgaggcgggg cacgctgtgc
      241 agcgtggtct tgccctcggc cagcaggcag gccgccacga tgggcagggc actgttcttg
      301 ctgccggaga tctcaatttt gcctttgagg cggctgccgc cgcgaatcag aaatgaatcc
      361 atcctgccca tccttgggcc cgaggcgggt gcctcgcgtg gcccgaataa gaagcgtgga
      421 attcggccaa acgagccgtc atcccacgaa tcattcggtc tatcggtcag tccattaacc
      481 gatcaacaat gtctgcattg ccaaggcgtt gccgcccggt tatcggcccg gcgcggtcta
      541 cttgccggac gtcgaacgcg gatgattctg ccacgttgag aatggaattc aagggacggc
      601 gacgactttt tgtggacggc gtggtttagg cgccgcggcg tccccgcgct tctcgacatc
      661 cgcgccccga ggcccgcggc gtccaaatgc gaggcgtcgg cgcgcttact ttaccgtctt
      721 gctgttcgaa tggtgtgaaa cagcgccctg gcgcgcggtg atctcgtcgc gcagcttcgc
      781 ggcatcctca taccgttcgt ctttcaccgc gcgatctaag cggcggcgga gctgctgcat
      841 cggatcgacc ggcagcttct tgcggatctc tctcgaaaat cgcttgagca cgcgcacctc
      901 gtttgatctt gcgaaggctt cttcctggcc gaactgttcg tagaactcgt ggatgcgggc
      961 cagcccggct tccaccgtct tgatggcggc gtcaaattcc ttgccctgca cgtggatcga
     1021 cgcttgggct cgcgcgttca tcatcgtgat gtacgggcga tattgctcga ggatcaggcg
     1081 gtcctgttcc tcaacggcga attggctgca caggtccagc acgcgcaggt tccgcgcggt
     1141 gtcgcggacc accccggtga aatcttccag gacgaaaagg ctgagatagc ggtgataata
     1201 catcaccgct tcttcgcgga gcgattggca ctcatcgccg gtcagttgga agccgtcttc
     1261 gctgccgtgc ttgcgcttgt actcttgaag ctccagctcg tggtgttcca gcaatgactc
     1321 gcgaccgaac ggccgatcac catcggggcg acctgtcact tccatctgca gcagccccag
     1381 gtccaggcgc atctgcagct tgggccggcc gtcgagaccg ctgaccttgc gcacgttgat
     1441 cgtgccggct tcgtagtccc agtcgcgcag gacgggcgtg acgtctttat cggaagcctt
     1501 cgcttcctgt ttatcgttcg gcggctttcg ctctttcatt ttcccaactc cagcacgcgc
     1561 ccgcgaccgc tgcacaaagc accatctgcg atcactggcc ggcgaccata gtatagcatc
     1621 attcgagcgc gtttttcgcc catcgacggc tggcgcgaca cctgtaaaaa tgggtgcttc
     1681 ttctcgtttg acgctggtga gggcgggcgc gaagatggcg ggcggcggtt gtgcccccgg
     1741 ccggaggtga atcatgggcg atgagtcggc caagtgtgcg tgccccagtt gtaactgccg
     1801 cgtgccgcca ggcaaaggcg tggtccgcga cggcaaggca tattgcagca gcgtttgcgc
     1861 gtacgaatgc acgccgacga cgtgcttatg cgtgcacgat cggtgcgagc gcgggaagta
     1921 gccttagcgc gtttcggaac catcacccaa agctcaaggc tctgccgcgg gaccgccttt
     1981 ctcaacgtct ggtcccacgg cagagccgtg ggcttttggg gttgtgaaaa tcggcgctaa
     2041 acctaaacga ggatcaggaa aaaccggatg cggatctgtc tctacaccgg caccgccctt
     2101 cccaaacttg gcgggcagga agcggtggtc gatgcgctgg cgcgacagtt ccagaagctc
     2161 ggccacgagc cgttcgtgat ggccccgcgg ccgcgcctgc cgctgcggcc gaatgacgcg
     2221 tcgctgccgt atccggtcat ccgccatccg cggttttact cgaccaaatt cctcgtcagc
     2281 tggtatcgcc gctggctgcg cgccgcatac cttcggcatc acttcgacgt gatccattgc
     2341 catgatgttt atcccaccgg ctatctcgcc gcgctcacga acaacgacct gcgcatcccg
     2401 atcgtcatca ccagccacgg cggcgacgtg cgcgaaggca atgttcgcct cagcaaaccc
     2461 gggatgcggc cgcggttcac gcgcgcggtg agcgacgccg atgcgctggt gtccatcgga
     2521 cggttcacgg aagaaggctt tcgccggctc ggcgcgaatg cgacgaagat cgtgacgatt
     2581 ccgaacggcg tggatctcga tccctttcgc gaacccgccg cgcgtccggc tgatctcgac
     2641 gcgcgaattg tccctgctga gtacgcgctc tttctcggcc gcctcgcgct tcgcaaaggt
     2701 gtggacactc tgctgcaggc gctggcgcag gtgacgccga atgggcgagt acagctggtg
     2761 atcgccggct ccggcgacga gcggccggca atcgagcaga tgatcgataa actcaatctg
     2821 cgcgatcgcg tgcgcctcgt cggtcgcgtc gtcggcgatc tgaagactta cctgctgcag
     2881 aacgcgctct gtacggtaat gccctcgcgc ggatgggaag cgttcccgct ggtcgtgctc
     2941 gaaagcttcg ccgccgggcg accggtgatc gggtcgcgca ttccgggcct ggaagatctc
     3001 atcgcgccgg atgtgacggg cctgctgttc acggatgaat ccgccgccga tcttgccggc
     3061 gcgct
//
LOCUS       DATIMH010000610         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1594100, whole genome shotgun sequence.
ACCESSION   DATIMH010000610 DATIMH010000000
VERSION     DATIMH010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594100"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VLI90_10540"
     CDS             complement(<1..435)
                     /locus_tag="VLI90_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14690.1"
                     /translation="MNCAIVDADANNRQELAAFLAQYGMNVVAQVAAIDGVTTLLGRQ
                     DAPQLVIVNLDPNANETLKKIGHFPRQFPATSFFVMSQIVDANLLMEAMHLGMKEFIP
                     LPIPEQKFAAAVERVASVHGMGKRARVISFIPTIGGVGSTTMA"
     gene            complement(520..>2018)
                     /locus_tag="VLI90_10545"
     CDS             complement(520..>2018)
                     /locus_tag="VLI90_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HSV14691.1"
                     /translation="GALIALLYTGAAAGADAPTQPSSDTAAAPQANGGRPSFVTDGLG
                     ADGSLRLVAGKGALLTTRSPYKRVSVGQPDIADVNTIGPVSVLVSAKKAGSTQLILWD
                     DQDRSQVVDIVVDMDLASAQRQLKMAFPESHIEASALNDRISLRGQVPSQQVAEQAVE
                     MVSAYGKVNNFLEISGGQQVMLQVRFAEVSKTASRDLGVNFGGTDGVSAFSTQSFGGG
                     INTFNLGSTDPAQRFLASQGDSTIFGAGRAGATTFDYFIKALRQNGLVRVLAEPNLVA
                     TSGQEASFLAGGQIPIPVPQPGNGGSTITIEYRDFGVRLNFTPQVLGNGRIRLKVSPE
                     VSDLDFSSAVTVAGTSVPGFTDRKLNTVVELADGQSFALAGLLNNRVSANKSAIPFLG
                     DIPVLGLLFRSVNYQRSETELVVIVTPRLVEAMNPDQVPLLPGEHWRYPDPLKFYGLA
                     DLGGPVVESDKAAADKANGKSAAAMPPPQFHGAYGYTPAGAGGAQVAQ"
BASE COUNT          344 a          674 c          645 g          355 t
ORIGIN      
        1 ggccatcgtg gtcgaaccca ccccgccgat cgtcgggatg aagctgatca cgcgggcgcg
       61 cttgcccatg ccgtgcacgg atgccacgcg ctcgacggcc gcggcgaact tctgttcggg
      121 aatcggcagc gggatgaact ccttcatccc caggtgcatc gcctccatca ggaggttggc
      181 atcgacgatc tggctcatca cgaagaagct ggtggccggg aactgccggg ggaagtgccc
      241 gatcttcttc agggtttcgt tggcgttggg atcgaggttg acgatgacca gctgcggggc
      301 gtcctgccgg ccgagcaatg tggtgacacc atcgatcgcg gcaacctgcg cgacgacgtt
      361 catcccgtat tgggcgagga acgccgccag ctcctggcga ttgttcgcgt cggcatcgac
      421 gatggcacaa ttcaattgaa cgtacatggc ggttcttcag gttcagctac aaatggttcg
      481 acccgcgcgc gcaccgttgg tccccgggga tttcaacgct tactgcgcca cctgcgcgcc
      541 acccgcgccc gccggtgtat agccgtaggc cccgtggaac tgcggcggtg gcatcgcggc
      601 ggcagacttg ccattggcct tatcggccgc ggctttgtcc gactcaacga ccggaccacc
      661 gaggtcggcc aaaccgtaga acttcagcgg gtccggatat cgccaatgct cgcccggcag
      721 cagcggcacc tggtcggggt tcatcgcctc gacgagccgc ggcgtgacga tcaccaccag
      781 ctccgtttcg ctgcgctgat agttcacgga ccggaacaac aggccaagca ccgggatgtc
      841 acccaggaac gggatggccg atttgttcgc cgacacgcgg ttgttcagca gccccgccag
      901 cgcgaagctt tgaccgtccg ccagctcgac caccgtattc agctttcgat ccgtgaatcc
      961 cggcaccgac gtgccggcga ccgtcacggc tgaggagaag tccagatcgc tcacttccgg
     1021 tgaaaccttc aggcgaatgc ggccgttgcc cagcacctgc ggcgtgaagt tcaagcgcac
     1081 gccgaaatca cggtattcga tcgtgatcgt tgacccgccg ttgcccggtt gcggcaccgg
     1141 gatcgggatc tgtccgccag ccaggaagct ggcttcctgc ccgctggtag cgacgaggtt
     1201 cggttcggca agcacgcgca ccaggccgtt ctgacgaagc gctttgatga agtagtcgaa
     1261 cgtcgtcgcg ccagcgcggc cggcgccgaa gatcgtcgaa tccccctggc ttgcgaggaa
     1321 gcgctgtgcc gggtcggtcg atccgagatt gaacgtgttg atcccgccgc cgaaggactg
     1381 cgtgctgaac gcgctgacac cgtcggtgcc gccgaagttc acacccagat cgcgcgacgc
     1441 ggtcttggag acttccgcaa atcgaacctg cagcatcacc tgctgcccgc cggaaatctc
     1501 gaggaagttg ttcactttgc cgtacgcgct gaccatctcg accgcctgct cggcgacctg
     1561 ttgcgacggc acctgcccgc gcagcgagat gcgatcgttc aacgcggacg cttcgatgtg
     1621 agactcgggg aacgccatct tcagctgacg ctgcgccgac gccaggtcca tatccacgac
     1681 gatgtcaacg acctgcgaac ggtcctggtc gtcccacagg atcagctgcg tgctgccggc
     1741 cttcttcgcg ctgaccagca cgctgaccgg gccgatcgtg ttgacgtcgg cgatgtcggg
     1801 ctggcccacg ctcacgcgct tgtacggcga gcgggtcgtc agcagcgcgc ccttaccggc
     1861 gaccagccgc agcgaaccat cggccccgag accgtcggtg acgaacgacg gccggccacc
     1921 gttggcctgc ggcgccgctg ccgtgtcaga tgacggctgc gtcggcgcat ccgcgccggc
     1981 cgcggcgccg gtgtacagga gcgcgatcag cgcgccgg
//
LOCUS       DATIMH010000611         6470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2455914, whole genome shotgun sequence.
ACCESSION   DATIMH010000611 DATIMH010000000
VERSION     DATIMH010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2455914"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1580)
                     /locus_tag="VLI90_10550"
     CDS             complement(240..1580)
                     /locus_tag="VLI90_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HSV14692.1"
                     /translation="MTKQTDVVVPAAAAATDNDAAARLRPMRRKIAEQISRDLGAAAA
                     GDSPRAEIQDRVTRAVAALNLAPGDRDALSKALMDELCGFGPIQPLLDDPSITEIMVN
                     RADRVYVERKGKPSRTAVVFDDDAHVRRIIDRIIVPLGRRLDASTPLVDARLPDGSRV
                     NAVIPPVAIAGPCLTIRKFSAARLTLDDLAGFGSLTPAIAQFLRGCVLARLNIVVSGG
                     TGSGKTTLLNALSACIPDDERIVTIEDAAELKLGQDHVITLEAKRPAVDGTGEVTIRD
                     LVRNALGMRPERIVVGECRGGEALDMLQAMNTGHDGSLTTLHANSPRDAISRIETMAL
                     MAGIDFPIRVIREQIGSAVQVLIQQARLRDGSRKITHVTEIAGMEGEKIVMQDIFKFR
                     EEPGLSSDGRVQGGLFAMGLRPHFIKKFENVGISLPAEMFSPSYTGGVVRAKAA"
     gene            complement(1817..3646)
                     /locus_tag="VLI90_10555"
     CDS             complement(1817..3646)
                     /locus_tag="VLI90_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14693.1"
                     /translation="MAQPPDAAPHLNRVTLLSSFALLASFATIAWLAARSKSPTYDEP
                     YHAPSAWVALHRGDFRIDSEDPPLWKYWAALPNGRDALRADFAGDVWRSIPSNLPRQW
                     YWCVTTLYRTPGNDAEAFIARSRAMMLIIGVALGALTAWWAWRLKGAVAAITAAGLFA
                     FDPNFLAHAPLVKNDVVFALSMLALLLVLWRCGQRLTWPRAIAAALLCAAMLTVKFSG
                     VLAGVLVPAMLGLRALLPWPWMVLRRELHHRLARLAAAVATTLLCAIVSIAGIWAVYG
                     FRFDPTPEPGVRLNTDQLAAMAAQNQAIAAGRSPQASAQLGAFVRTMRLAEAHHLLPQ
                     SWLAGLLFTYQASLVRPAYLFGRISPTGWWYYFPLAMLVKTPVATIVLAAFTLIAMTA
                     LLPRLIRTRAQLWNILCLATPFAIYMISAMRGHLNIGIRHMLPVYPLAFIAIGCAAAW
                     TVRRWGRRAVIVLCVFGVLLVGETLAAFPDYIPFFNLAAGGERGGFHLLGDSNLDWGQ
                     DLKALAEWQRAHPEMPLYLAYFGLADPHYYGVNYTPLPGGYRYDPTPQWPRGRCVVAV
                     SATYLQGLLVDPELYDTFYRKVAALRPDDVLGGSLYLYEFPPK"
     gene            3901..5526
                     /locus_tag="VLI90_10560"
     CDS             3901..5526
                     /locus_tag="VLI90_10560"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV14694.1"
                     /translation="MSLAVALNRQGQFDQSISICQRVTEIDPGFADAHFVLADSLRKA
                     VRREEAIAHYRRGIALRPDALGAYCDMARVLRQMGRLEESLEAFRQAIARNANWADAH
                     DGLGVALRDAGQIDDAIAAHRRAVDLAPQISTYHSRLLYAMHYSPRFDSAAIRDEHHR
                     WAEHHGNPLTAIAMSHANDRSPDRRLKIGYVSPDFKIHPVTLFLPSLLNNHDHAQFEV
                     YCYSDVRFPDAMTARIQTFADHWIDARGMSDAALAERIRSDRIDILIDLTLHMASNRL
                     LMIARKPAPVQITWLAYPSTSGLKAMDYRISDPWLDPPDQDINYTERTIRIPTYWCYQ
                     PFDNPPDVSPLPSAANGYITFGCLNNFAKVTVPTLELWADVLRRVDRSHLVILAKPGE
                     HRQRTVQALVDRGIDAQRISFVGMLPHSEYLKQYHRIDIVLDTVPYNGHTTTLDALYM
                     GVPVVTLRGPTAVGRGGVSILSQLGLPELIAQSPAEYASIAESLAAAGSRLSALRSDL
                     RRRISESTLMNARQFTDSMEAAYRQAWRAWVER"
     gene            complement(5666..5752)
                     /locus_tag="VLI90_10565"
     tRNA            complement(5666..5752)
                     /locus_tag="VLI90_10565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5716..5718),aa:Ser,seq:cga)
     gene            5882..>6470
                     /locus_tag="VLI90_10570"
     CDS             5882..>6470
                     /locus_tag="VLI90_10570"
                     /inference="COORDINATES: protein motif:HMM:NF039976.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HSV14695.1"
                     /translation="MLHYFKFRQDLFAPVAGRDVYVKRPAGRGWPEECPPMRAANAFG
                     FDLLANFDVTFVRQRGGDWRVLKDVEIQSDFDFAASDDAAGSPLTQRYAWFWERGQRI
                     PHVISDNVYRVIRNQVKVSSYLFLQTDPNEVLLMTEVPNLRRPWRAMTALIETDWYPA
                     SYPWHCVIELDEAERRITIKKGEPLCRVIPVRRDTY"
BASE COUNT         1231 a         2054 c         2026 g         1159 t
ORIGIN      
        1 ggatttcttc tcgatcgccg agtcgaacaa caacagcgag atcgacatca cttacgtgcc
       61 ggaaccggcg agcgcgggcc tgctgaccat cgcggcgatc ggcatgctgg gccggcggtc
      121 gcggcgatcg gtgatgcggg atgcgtaatt cgtaattcgt aatgcgtagc tcgtaaccgg
      181 acgctttagg cgttacgaat ggcgcgctac gaattacgcg tttcctgcgc ccccgcccct
      241 cacgcggcct tcgcgcgaac gaccccgcct gtgtacgacg ggctgaacat ctccgccggc
      301 aggctgatgc cgacgttttc gaacttcttg atgaagtgcg gccgcaggcc catggcgaac
      361 agcccgccct gcacgcgccc gtcgctgctc aggcccggtt cttcgcggaa cttgaagatg
      421 tcctgcatga cgatcttttc gccttccatc ccggcgattt ccgtcacgtg ggtgatcttg
      481 cgcgagccgt cgcgcaggcg ggcctgctgg atcagcacct gaacggccga gccgatctgc
      541 tcgcggatga cgcggatggg aaagtcgatg cccgccatca gcgccatcgt ctcgatgcgc
      601 gagatcgcgt cgcgcgggct gttggcgtgc agcgtcgtca acgagccgtc gtggcccgtg
      661 ttcatcgcct gcagcatgtc gagcgcttcc ccaccgcggc attcgccgac gacgatgcgc
      721 tccggccgca tccccaaagc attgcggacc aggtcgcgaa tcgtcacttc gcccgtgccg
      781 tcaaccgcgg ggcgcttggc ttccagcgtg atgacgtgat cctgtcccag cttcagctcg
      841 gccgcatctt cgatggtgac gatgcgctcg tcatccggaa tgcacgccga cagcgcgttg
      901 agcagcgtcg tcttgccgct gccggtgccg ccgctgacga cgatgttgag ccgcgccagc
      961 acgcaaccgc gcaggaactg tgcgatcgcc ggcgtcagtg aaccgaaccc ggccaggtcg
     1021 tccagcgtca ggcgcgctgc gctgaacttg cggatcgtga gacatggccc ggcgatagcc
     1081 accggcggaa tgaccgcgtt cacgcgcgac ccatccggca gccgcgcatc gaccagcggc
     1141 gtggacgcat cgagccgccg gcccagcggc acaatgatgc ggtcgatgat ccgccgcacg
     1201 tgcgcgtcat cgtcgaaaac gaccgcggtg cgcgacggct ttcccttgcg ctcgacgtac
     1261 acgcggtcgg cgcgattgac catgatttcg gtgatgctcg gatcgtccag cagcggctga
     1321 atcgggccga agccgcacaa ctcgtccatc agcgctttgg acaacgcatc gcgatcgccg
     1381 ggcgcgaggt tcagcgcggc cacagcgcga gtcacgcgat cttgaatttc cgcgcgcggc
     1441 gaatcacctg cggcggcggc gccaagatcg cgcgagatct gctcggcgat cttccgccgc
     1501 atcggacgca gcctcgcggc agcgtcatta tcggtcgcgg cggcggcggc ggggacaacg
     1561 acatcggttt gcttcgtcat gacagattta catcgggcga ttccctacct ccgatgaatt
     1621 ggcccagcta catcatttgg ctgcgacgat tgattttggt agatcgtctg tgcgccaaga
     1681 cgccaaggaa ttaaaggcaa tgctttactg gtagcacggg cgtttcacac gttccatctt
     1741 tctcttcctt ggcgctcttg gcctcttggc gtcttggcgc tgaaagacgc atgccaatat
     1801 tgtgcgctac agcccatcat ttcggtggga attcatacaa atacaatgat ccgcccagca
     1861 cgtcatcggg ccgcagtgcc gccaccttgc gatagaacgt gtcgtaaagt tctggatcga
     1921 ccagcaggcc ttgcaaatac gtcgcgctga cggcgacaac gcaacggccg cgcggccact
     1981 gcggtgtcgg atcgtaacga tacccacctg gcagcggcgt gtagttaacg ccgtaataat
     2041 gcgggtcggc caagccgaag tacgcgaggt acagcggcat ctccgggtgt gctcgctgcc
     2101 attccgccag cgctttgagg tcctgtcccc agtcgagatt ggaatcgccc agcagatgaa
     2161 accccccgcg ttcgccgccg gcggcgaggt tgaagaacgg gatgtaatcg ggaaaggcgg
     2221 cgagcgtttc ccccaccagc agcacgccga aaacgcaaag cacgataacc gcgcgacgcc
     2281 cccaccgccg cacggtccac gcggcggcgc agccgatcgc gatgaacgcc agcgggtaga
     2341 ccggcagcat gtggcggatg ccgatgttca ggtggccgcg catggcggag atcatgtaaa
     2401 tcgcgaaggg cgtcgcgagg cacaggatgt tccacagctg cgcgcgcgta cgaatcagcc
     2461 gcggcaagag cgcggtcatt gcgatgagag taaatgcagc gagaacgatg gttgcgaccg
     2521 gcgtcttcac gagcatcgcc agcgggaagt aataccacca gcccgtcggc gagatccggc
     2581 caaacaggta cgccgggcga accagtgacg cttgatacgt gaacaacagc cccgccagcc
     2641 aggactgcgg gagcaggtga tgcgcttcgg cgaggcgcat cgttcgcacg aaggcgccga
     2701 gttgagcaga tgcctgcggc gatcgccccg ccgcgatggc ttggttttgc gcggccatcg
     2761 ccgccagctg atcggtgttg agccgcacgc ccggctcagg ggtgggatcg aaccgaaatc
     2821 cgtataccgc ccagatgccg gcgatgctga cgatcgcgca caagagcgtc gtcgcaaccg
     2881 cggcagctag tcgcgcgagt cgatgatgca gctcgcgcct caaaaccatc cagggccacg
     2941 gcagcagcgc gcgaaggccg agcattgcgg gcaccaacac gcccgccagc acgccggaga
     3001 atttcaccgt cagcatggcc gcgcacagca acgccgccgc gatcgcccgc ggccaggtca
     3061 accgctgccc gcatcgccat agcaccaaga gcaatgcgag catcgacagc gcgaagacga
     3121 cgtcgttctt caccagcggc gcgtgcgcga ggaagttcgg atcaaacgcg aacaaccccg
     3181 ctgcggtgat ggccgcaacc gcgcccttca accgccacgc ccaccacgcc gtcagcgcgc
     3241 ccagcgccac gccgatgatc agcatcatcg cccgcgagcg cgcgatgaac gcctcggcat
     3301 cattgcccgg cgtgcgatag agcgtcgtca cgcaccagta ccattgccgc ggcagattcg
     3361 aaggaatgct ccgccagaca tcaccggcga aatcggcgcg gagcgcatcg cgcccgttcg
     3421 gcaaggccgc ccagtatttc caaagcggcg ggtcttcgct gtcgatgcga aaatcccctc
     3481 gatgcaacgc cacccacgcc gacggggcat gatacggctc atcgtacgtc gggcttttgc
     3541 ttcgcgccgc cagccaggcg atcgtcgcaa aagatgcaag cagcgcaaaa ctcgacaaca
     3601 atgtgacgcg attcaaatgc ggcgcggcat cgggaggttg agccatcggc cggacagtat
     3661 aaggtcgcgc atgacgaaca ggcgatccca cgccgggttc ggaggtactc cgaacccggc
     3721 gtggaggcgg gcgacgcttt tgaacgtctc acgaccattc agatttccgt ggtcgcattc
     3781 agttcctcag caatcagtga cttggcaccg cgcttcgccg tagcgatgat ccgacagtcg
     3841 tttcactcgg acgccgcaaa acaaatgccc actccaacgc ctctttcgat cgagcaagcg
     3901 atgtccctgg ccgtggcctt gaaccgacag gggcagttcg atcagtcgat atctatctgc
     3961 cagcgcgtca ctgaaataga tcccggattc gccgacgctc atttcgtgct ggccgacagc
     4021 ctgcgcaagg ccgttcgcag agaagaggcg atcgcccatt accggcgggg catcgcactt
     4081 cgccccgatg cgctcggcgc ctactgcgac atggcgcgcg tgcttcgaca gatgggcagg
     4141 ctcgaggaat ccctcgaggc gtttcgccaa gcgatcgcgc gcaatgccaa ttgggccgat
     4201 gcacacgacg ggctgggcgt ggcgttgaga gacgccggtc agattgatga cgcgattgca
     4261 gcgcatcgcc gcgccgtcga tctcgcgccg cagatctcga cgtatcacag ccggctgctc
     4321 tacgcgatgc attacagccc gcggttcgac agcgcggcca tacgtgatga acaccaccgg
     4381 tgggccgaac accatggcaa tccactcaca gccatcgcca tgtcgcacgc caatgaccgt
     4441 tcgcccgatc gccgcttaaa aatcggatat gtctcgccgg actttaaaat ccatccggtg
     4501 acgctcttcc tgccgtcgct gctgaacaac cacgaccacg cacagttcga ggtgtactgc
     4561 tacagcgacg tgcgctttcc cgacgcgatg accgcgcgaa tccagacgtt tgccgaccat
     4621 tggattgacg cgcgcggcat gtccgatgcc gcactggccg agcgcatccg ctcagaccgc
     4681 atcgacatcc tgatcgacct aacgctgcac atggcgtcga atcgcttact gatgatcgcg
     4741 cgaaagccgg cgcccgtgca aatcacctgg ctcgcttacc ccagcacgtc gggcctcaag
     4801 gcgatggact atcgcatcag cgatccgtgg cttgatccgc cagaccagga catcaactac
     4861 accgaacgaa cgatccgcat cccgacgtac tggtgctatc aaccattcga caatccgccg
     4921 gacgtttcac cgctgccgtc ggccgccaat ggctacatca cgttcggttg cctgaacaat
     4981 ttcgcgaagg tcaccgtgcc gacgcttgaa ttgtgggccg acgttctgcg gcgcgtcgat
     5041 cgctcgcacc tggtgatcct tgccaagccg ggcgagcatc gacagcgcac cgtccaggca
     5101 ttggttgacc gcggcattga tgcgcagcgc atctcgttcg tcggcatgtt gccgcattcc
     5161 gaatatctca agcagtatca tcggatcgac atcgtgctgg acaccgtgcc gtacaacggg
     5221 cacacgacaa cgctggacgc gctgtacatg ggcgtgccgg tcgtgacact acgcggccca
     5281 accgccgtgg gtcgcggcgg agtgagcatc ctgtcacagc ttggcctgcc cgaactgatc
     5341 gcgcaatcgc cggcggagta cgcaagcatc gccgaatcgc tggcggcggc cggcagccga
     5401 ttgtctgcgc ttcgttcgga cttgcgcagg cggatatctg aatccacgtt aatgaatgcc
     5461 cgccagttca ccgacagcat ggaagcagca tatcggcagg cgtggcgcgc gtgggtcgag
     5521 cgctaggatt tacggcagat ttcgccgcaa agcgccgttt actcaatctg atctggttga
     5581 acttggtact gatgggcgca actctcgcca tttcatgcag aaagcccttc aactcattgg
     5641 tcgaagggct ttctgtctcg gaaagcggag agggggagat tcgaactccc gcgaccattg
     5701 ctggccgacc ggttttcgaa accggcgcat tcagccgctc tgccacctct ccagcggcgg
     5761 acggcggtac gttagcttag cgattattct gggtcaaact caggcgcggc atcggcacgc
     5821 ggcggcggcg gcgtgacgtc gggcgtttgg cgggggcgcg cggcggcggt aacgtgccgc
     5881 catgctgcat tacttcaagt ttcggcagga cctgttcgcg ccggtggcgg ggcgggatgt
     5941 ttatgtgaag cgcccggcgg gccgcgggtg gccggaggag tgcccgccga tgcgagcggc
     6001 caacgcgttt gggtttgatc tactggcgaa ttttgacgtg acgtttgtgc ggcagcgagg
     6061 cggggattgg cgcgttctga aggacgtgga gatccaaagt gactttgatt tcgcggcgag
     6121 cgacgacgcg gcgggttcgc cgctgacgca gcggtatgcg tggttctggg aacgcgggca
     6181 gcggattccg catgtgattt cggacaacgt gtatcgcgtc attcggaacc aggtgaaggt
     6241 cagcagctat ttatttttgc agacggaccc gaatgaggtg ttgttgatga cggaggtgcc
     6301 gaaccttcgc cggccgtggc gggcgatgac ggcgttgatc gagacggatt ggtacccggc
     6361 cagttatccg tggcattgcg tgattgagtt ggacgaggcg gagcggcgga tcacgatcaa
     6421 gaagggggag ccgctgtgcc gggtgattcc ggtgcggcgg gatacatatt
//
LOCUS       DATIMH010000612         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1015344, whole genome shotgun sequence.
ACCESSION   DATIMH010000612 DATIMH010000000
VERSION     DATIMH010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015344"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="VLI90_10575"
     CDS             <1..1668
                     /locus_tag="VLI90_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HSV14696.1"
                     /translation="TMGLKEMIQHFIAHRKEVITRRTKFQLKKALQREHILEGTIFAV
                     ADIDEVIRLIRSSKTREEAIEKLMAQGFRITPGSEVLSKVPERIRFKHPEIFALEPGM
                     PMPPYRVSRAQAEAIGRLQLIQLVGLEIDKLVAEYKEVAEHIEEYERILGDEKYLMDI
                     IVTDTLEMRDKYGDDRRTEITGDALDVNMEDLIAQEDVVVTVSHAGYVKRLPVDTYRS
                     QGRGGRGIKGTESKEGDFVEHLFVANTHDYLLFFTNQGRVYERRVFDIPEMSRTSQGR
                     SIANLLSFQPEEKVANVLAIKDFSSGEHFLMFATAKGTVKKTALSAYANIRQSGLIAI
                     GLDEGDSLIGVEITSGKDHIILGTKSGLAVRFEESDVRAMGRPAGGVTGARFKREGDA
                     VVSMIIIPNGDNACCQVMTACVNGYGKRTPLSDYPVKGRGTRGVINIDANERNGDVIS
                     LQLVVAEDELMLITQAGILIRTKVAEIRETGRNAAGVRLIKLDDGDKLVAMARVDAEE
                     KVVEAGEGGASPAPEPTDSAPPAPPAPSNGDGQAPSANGDGDGQPTE"
     gene            complement(1707..2063)
                     /locus_tag="VLI90_10580"
     CDS             complement(1707..2063)
                     /locus_tag="VLI90_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV14697.1"
                     /translation="MRRLKNGAEHAQAKRLYLRLSTGKADQGKRQYIQVLVDLMGEYE
                     RRAGFALDTSKLTAVDLIQHRLEARGMSVAALAKEVGIPQPNLSAMLAGKRDWSKAAI
                     RSLSAFFHIRAERFLQ"
     gene            complement(2132..2320)
                     /locus_tag="VLI90_10585"
     CDS             complement(2132..2320)
                     /locus_tag="VLI90_10585"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HSV14698.1"
                     /translation="MREDYASADGVKLRSLIVVTVFNVKGNEYRLLTQVNYRDRVVLV
                     LELLTHAEYDKDAWKGRY"
     gene            complement(2327..2746)
                     /locus_tag="VLI90_10590"
     CDS             complement(2327..2746)
                     /locus_tag="VLI90_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14699.1"
                     /translation="MSNAAATEKQIPTALIIVGAGLWVLAGFMGYGVRGASNALAAVG
                     LIATVNVVLMIAGAFLTALVLRISFGDLQSAILKLGAIALFPGAVGYLIPGAGGLIAL
                     ALYFSLLIWFFELEVYQAIVFTIVLFVIRLLVAAMFV"
     gene            complement(2830..3357)
                     /locus_tag="VLI90_10595"
     CDS             complement(2830..3357)
                     /locus_tag="VLI90_10595"
                     /inference="COORDINATES: protein motif:HMM:NF027587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4916 domain-containing protein"
                     /protein_id="HSV14700.1"
                     /translation="MAMTMNRNWLGDDDWRWVQETVPVACVDVLPVQMSGVGGAIARV
                     GLIHRDTPHQGRRWCLVGGRLWRDESFAEAATRQIRETLGAGAQFHIDADAQPVYIAQ
                     YFTHEREVGLLDPRQHAVAMVFVVPIGGDIVAGGEACEFRWFGRNELPIPEEWGFGQD
                     RVAEACLKPWERARV"
     gene            3649..>3923
                     /locus_tag="VLI90_10600"
     CDS             3649..>3923
                     /locus_tag="VLI90_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14701.1"
                     /translation="MKLALRTAACGLAVTLLASAAWADCKIDNQDDKDYKVTYKNVGS
                     SSSTETTINHHTASNQTTSGDIEIEVDGVGKITAGNDDRVIIKDGKLT"
BASE COUNT          844 a         1325 c         1151 g          603 t
ORIGIN      
        1 acaatggggc tgaaggagat gatccagcac ttcatcgccc accgcaaaga ggtcatcacc
       61 cggcggacga agttccagct gaaaaaggcc ctgcagcgcg agcacattct ggaaggcacg
      121 atcttcgccg tcgccgacat cgacgaagtg atccgcctga tccgcagcag taagacgcgc
      181 gaagaggcga tcgagaaatt gatggcccag ggcttccgca tcacgccggg cagcgaagtg
      241 ctgagcaagg tgccggagcg catcaggttc aagcatccgg agatcttcgc gctggaaccc
      301 ggcatgccga tgccgccgta tcgcgtgtcg cgcgctcagg ccgaagcgat cggccggttg
      361 cagctgatcc agctggttgg gctcgaaatc gacaagctgg tcgccgagta caaagaggtg
      421 gccgagcaca ttgaagagta cgagcgcatc ctcggcgacg aaaaatattt gatggacatc
      481 atcgtgaccg acacgctgga gatgcgcgac aagtacggcg acgaccgccg cactgaaatc
      541 acgggcgacg cactcgacgt gaacatggaa gacctgatcg cgcaggaaga cgtggtggtt
      601 accgtgtcac acgcggggta cgtcaagcgg ctgccggtgg acacctaccg cagccagggc
      661 cgcggcggcc gcgggatcaa gggaaccgaa tcgaaggaag gcgatttcgt cgagcacctg
      721 ttcgtcgcca acacgcacga ttacctgttg ttcttcacga accagggccg cgtgtacgaa
      781 cgccgcgtgt tcgacatccc ggaaatgagc cggaccagcc aggggcgaag catcgcgaat
      841 ctcctctcgt tccagccgga agagaaggtc gcgaacgtgc tggcgattaa ggatttctcc
      901 agcggcgagc acttcctgat gttcgccacc gccaagggga cggtgaagaa gaccgcgctc
      961 agcgcctacg cgaacatccg ccagagcggc ttgatcgcga ttggcctgga cgaaggcgat
     1021 tcgctcatcg gcgtcgagat caccagcggc aaggaccaca tcatcctcgg caccaagagc
     1081 ggtctggccg tgcgattcga agaatccgac gtccgcgcca tgggccgccc cgccggtggt
     1141 gtcacgggcg cgcgcttcaa gcgcgaaggc gacgcggtcg tcagcatgat cattatcccc
     1201 aacggcgaca atgcctgctg tcaggtgatg accgcctgcg tgaacggcta tggcaaacgc
     1261 acgccgctca gcgattaccc cgtcaaaggc cgcggcaccc gcggggtcat caacatcgac
     1321 gccaacgaac gcaacggcga cgtcatcagc ctgcagctgg tcgtcgccga ggacgagctg
     1381 atgctgatca cacaagcggg catcctcatc agaacgaagg tcgccgagat ccgcgaaaca
     1441 ggaagaaacg ccgccggcgt gcggctgatc aagctggacg acggcgacaa gctggtcgcg
     1501 atggcccgtg tggatgccga ggagaaggtg gtcgaggcag gggaaggcgg ggcgtcaccc
     1561 gcgccggagc cgactgattc agcgcccccc gccccccccg cgccctcgaa cggcgacggc
     1621 caagccccca gcgccaacgg ggacggcgac ggacagccaa cggaataaca aacacgatcc
     1681 cggcgtctct gcgcgccctc gagaacctat tgcagaaacc gctccgcgcg gatgtgaaag
     1741 aacgccgaca gcgatctaat cgctgccttg ctccaatcgc gcttccccgc cagcatcgcc
     1801 gaaaggttcg gctgcggtat gccgacttcc ttggctagcg ctgcgaccga catcccgcgc
     1861 gcttccaagc gatgctgaat caaatcgacg gctgtaagct tcgacgtatc aagcgcaaaa
     1921 cccgctcgcc gttcgtattc ccccatcaag tccacgagca cctgaatgta ctggcgcttc
     1981 ccttggtcgg ccttgcccgt ggagagccga aggtaaagcc tcttcgcctg tgcatgctcc
     2041 gcgccatttt tcaggcggcg caaggggaac tcgcgaacga gatcgaaata tgtatctgag
     2101 acgggccgtg aatttgccgt acgcatggcg actaataccg tcctttccag gcatccttgt
     2161 cgtactcggc atgtgtcagc agttcgagaa ccagcacgac ccgatctcga taattcacct
     2221 gcgtgagcag ccgatactcg tttcccttga cattgaagac ggtgacgaca atcaggctgc
     2281 ggagcttcac gccgtccgcc gacgcataat cctcgcgaat gcgccgtcac acgaacatcg
     2341 ccgccacgag cagccggatc acgaacagca caatcgtgaa cacgatcgcc tggtagactt
     2401 ccagttcgaa gaaccagatc agcagcgaga agtagagcgc cagcgcgatc agcccgccgg
     2461 cgccggggat gagatagccg accgcgcccg ggaagagcgc gatcgcgccg agcttcagga
     2521 tggccgactg aagatcgccg aaggagatgc gcagcaccag cgcggtgagg aacgccccgg
     2581 cgatcatcag cacgacattc accgtcgcga tcaggcccac ggcggccagc gcgttgctcg
     2641 ccccgcggac gccgtagccc atgaatcctg cgagcaccca gagcccggcc ccgacgatga
     2701 tcagcgccgt ggggatctgc ttttccgttg ccgccgcgtt cgacatggct gccctttcgc
     2761 aagttgccga tcgttattaa tgacgctcat cgtgacacgg cctccggccc gtgcgagcac
     2821 gccgcagcgt cagacccgcg ccctctccca tggtttcagg caagcctccg ccacccggtc
     2881 ctgcccaaaa ccccattctt ccggtatcgg caattcattc ctgccaaacc agcggaactc
     2941 acacgcctct cccccggcga cgatgtcgcc gccgatcggc accacgaaca ccatcgccac
     3001 cgcatgctgc cgcgggtcga gcaggccgac ttcgcgctcg tgcgtgaagt attgcgcgat
     3061 atataccggc tgcgcatccg catcaatgtg gaactgcgcc cccgccccca acgtctcgcg
     3121 gatctgtcgc gtcgcggctt cggcgaacga ttcatcccgc cacaaccgcc cacccacgag
     3181 gcaccaccgc cgcccctggt gcggcgtgtc gcggtggatc aaaccgaccc gagcgatcgc
     3241 gccgcccacg ccgctcattt gcacaggcag cacgtccacg caggcgaccg gcaccgtctc
     3301 ctgcacccac cgccaatcgt catcgccaag ccagttgcgg ttcatggtca ttgccattcc
     3361 aacgtctcgt tttcgatcgt cacgctccaa gcgccggcta agctgccggt cccctctccc
     3421 ggtactgctg accgcaggtc gccgggagag ggagtaagac aacgcgcggc actcgccgta
     3481 cgaagaacca ttgccgcccg cgcctccaaa gagtatgaca cccagcgcga aaggaaaggt
     3541 gtcggcaccc gagactcccc gggcgaaggg taatccggcc gtccggccgg ctcttcgccg
     3601 cgcgcggttc attctcggga cgccgtatgc ctaacatcgg tgaaccccat gaaactcgcc
     3661 ctccgcaccg ccgcctgcgg cctcgccgtc accctgctcg cctccgccgc ctgggccgat
     3721 tgcaaaatcg ataaccagga cgacaaggac tacaaggtca cctacaaaaa cgtcggcagc
     3781 agcagctcaa ccgaaacgac gatcaaccac cacacggcca gcaatcaaac caccagcggc
     3841 gacatcgaga tcgaagtgga cggcgtcggc aaaatcaccg ccggcaacga cgaccgcgtg
     3901 atcatcaagg acgggaagtt gac
//
LOCUS       DATIMH010000613         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3613879, whole genome shotgun sequence.
ACCESSION   DATIMH010000613 DATIMH010000000
VERSION     DATIMH010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3613879"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /gene="queA"
                     /locus_tag="VLI90_10605"
     CDS             <1..898
                     /gene="queA"
                     /locus_tag="VLI90_10605"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HSV14702.1"
                     /translation="LRPTDVLVFNDAKVVPARFMLQKSTGGRIEGLFLGELSPGEWSV
                     LLRNLGDAPVGTTLRFVDAPEVEAKLIARGEAGEHRLAIAAGEPALLFLNRVGRMPLP
                     PYIKRERGHDARDDADRERYQTVYARAAGSVAAPTAGLHFTDAILGAIDAMGVQRVFL
                     TLHVGLGTFKPVTADRLEDHAMHTESFSVDAAAAEALNRAERDGRRIVAVGTTAARVL
                     ESWPADEPWREHAGTTSIFIYPPYQWRRVGALVTNFHLPRSTLIALVAAMVGLEEQRR
                     LYRIAIEQRYRFFSYGDAMFIE"
     gene            complement(1001..>2083)
                     /locus_tag="VLI90_10610"
     CDS             complement(1001..>2083)
                     /locus_tag="VLI90_10610"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HSV14703.1"
                     /translation="KAADWQIAALPTQKAYKQDTANGWIRAPFYVGVMSLHDTLKDEQ
                     HYLNAMMDIAEQCHWELANRKYPPGGTTVPSSWSTTQAALDAGILQPGWPFLRHADDL
                     AMGQLYCELYFIKRDSHMIESLQKRADQLMSRPQPGRDEWWWCDSLFMAPPTLARLSA
                     ATGDRKYLDYMDKQWWDATDLLLDKHEHLYFRDRSYFKKAEKNGQKVFWSRGNGWVVA
                     GIVRILQYMPEDYPSRPKYIALLKEMSERIVTLQQPDGFWRASLLDPASYPGGETSGT
                     GFFCYAMAWAINHNLLPRDQYLPAAQNAWRALKSAEQPDGKVGWIQPVGASPAALKAD
                     DTAQYGVGAFLLAGTEVLKLDARAPQ"
BASE COUNT          335 a          615 c          725 g          408 t
ORIGIN      
        1 cctgcggccg accgatgtgc tggtcttcaa cgacgccaag gtcgtcccgg cacggttcat
       61 gttgcaaaaa agcaccggcg ggcggatcga agggcttttc ctgggcgagc tgtcgccggg
      121 cgagtggtcg gtgctattga ggaacctggg cgatgccccg gtcggcacga cgctgcggtt
      181 cgtcgatgcg ccggaggtcg aagcgaagct gatcgcgcgc ggcgaggccg gcgaacatcg
      241 gctggcgatc gcggcggggg aaccggcgct gcttttcctg aaccgcgttg gccggatgcc
      301 gctgccgccg tacatcaagc gcgagcgcgg acacgatgcg cgggatgacg cggatcgcga
      361 gcggtatcag acggtgtacg cgcgggcggc cgggtcggtc gccgccccga cggcgggttt
      421 acattttacg gatgcgatcc ttggcgcgat cgacgccatg ggcgtgcaac gcgtgttcct
      481 cacgctgcac gtcggacttg gcacgttcaa accggtcacg gccgatcgcc tcgaagatca
      541 cgcgatgcac acggagtcct tcagcgtgga tgccgccgcg gcggaggctt tgaatcgcgc
      601 ggagcgcgat ggccggcgga tcgtcgcagt tggcacgacg gccgcccgcg tgctggagag
      661 ttggccggcg gatgagccgt ggcgcgagca cgcgggaacg acgtccatct tcatctatcc
      721 gccataccaa tggcgacggg ttggggcgct ggtgacgaat tttcatctgc cgcggagcac
      781 gctcattgcg ctcgtcgcag cgatggtggg attggaagag cagcggcggc tgtaccggat
      841 cgcgatcgag cagcggtatc ggttcttcag ttacggggat gcgatgttca tcgagtgaca
      901 gtcaatcaat ttaatagccg gggccggaag gcccggtgtt ttatttggac agagaaagag
      961 aaaagaccgg ggcttccgcc cccggctatg aaggattctg ttactgcggc gctcgtgcgt
     1021 cgagcttgag cacttccgtt ccggcgagaa gaaacgcgcc gacgccgtat tgggcggtgt
     1081 catccgcctt cagcgccgcg ggcgaggcgc cgacgggttg gatccagccg actttgccgt
     1141 cgggttgctc ggcgcttttg agtgcccgcc aagcgttctg cgcggccggc aggtattggt
     1201 cgcgcggcag caggttgtgg ttgatggccc aggccatcgc gtagcagaag aacccggtgc
     1261 cgctggtctc gccgccgggg tagctggcgg ggtcgagcaa gctcgctcgc cagaaaccgt
     1321 ccggttgctg gagcgtcacg atgcgctcgc tcatctcctt cagcagcgcg atgtacttcg
     1381 gccggctggg gtagtcctcg ggcatgtatt gcaggatgcg gacgatgccg gccaccaccc
     1441 acccgttgcc gcggctccag aacaccttct ggccgttctt ctcggccttt ttgaaatacg
     1501 agcggtcgcg gaaatacagg tgttcgtgct tgtcgagcag caagtccgtc gcgtcccacc
     1561 actgcttgtc catgtagtcg agatattttc gatcgcctgt tgcagcgctg agtcgcgcca
     1621 acgtcggcgg ggccatgaac agcgaatcgc accaccacca ttcatcgcgc ccgggttgcg
     1681 ggcgcgacat cagctgatcg gcgcgcttct gcagtgactc gatcatgtgc gagtcgcgct
     1741 tgatgaagta cagctcgcaa tagagctggc ccatggccag gtcgtcggca tgacgcagga
     1801 acggccagcc gggttggagg atgccggcgt ccagcgcggc ttgcgtcgtg gaccaactcg
     1861 acggcaccgt tgtgcccccg ggtgggtact tgcggttggc cagttcccag tggcactgct
     1921 cggcgatgtc catcatcgcg ttcaagtaat gctgctcgtc tttgagcgtg tcgtgcagcg
     1981 acatcacgcc gacgtagaac ggggcgcgga tccagccgtt ggcggtgtct tgtttatatg
     2041 ccttttgcgt gggcaacgcg gcgatttgcc agtcggcggc ttt
//
LOCUS       DATIMH010000614         4076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2168549, whole genome shotgun sequence.
ACCESSION   DATIMH010000614 DATIMH010000000
VERSION     DATIMH010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2168549"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1913)
                     /locus_tag="VLI90_10615"
     CDS             complement(<1..1913)
                     /locus_tag="VLI90_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSV14704.1"
                     /translation="MLLVGGNWKVQSASLVLSSPKQIARAEFDDRQWVPATVPGTVLS
                     SYLDAGKIPDPYYADQRYQIDDAFFTDNDFWYRDTFAVPKEFTGHRIWLNFDGINWKA
                     DIFVNGSRVGRIDGAFLRGRFDVTPLVKPGEKMCLAVLVHKVAHPGEVKHKSLAKWPP
                     NGGILGLDSPTFVSSIGWNWLPTIPGRNVGIWNDVWLSSSGDVSIVDPFVTTELPSPD
                     VAEITARVELVNHSNQPQDGTVHGAIDNIEFSSPISLAASETKTITFDKTNCPQLVIR
                     NPKLWWPNGMGDQPIYHLKLRVDLANVTSDAKEIPFGIRKLTYQTHDNVLFIYCNGQR
                     VLLRGGNWGMDEGMLRCDDAGYELRVRLHKEMNFTMIRNWVGMTGRDGFYNACDKYGI
                     LIWDDFWLANPADGPDPSDEAMFMTNARDKIRRVRNHPSLALYCGRNEGYPPKSLDEQ
                     LHAATKELDPTRKYLSHSAADGATGFGPYDVHAPEWYFEHRGMTLHSELGIVAVPPVE
                     TLRTFLPPDKLWPINDLWGQHDLSQERGPAYMKRLNDSYGEASSVEDFCRKAQLLNLE
                     SAKAMLECWQSHQGSGGLIWMTQSAWPCLICQAYTYELEPTGAYFGFKKACEPVHILR
                     DSYTNRIKAANDTP"
     gene            complement(2058..2687)
                     /locus_tag="VLI90_10620"
     CDS             complement(2058..2687)
                     /locus_tag="VLI90_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HSV14705.1"
                     /translation="MSDSTTTAARDSGAPDVHPGLRSYLRRRRIVRIAAVVTITFAMV
                     SAGFSHLRAPPDDCARFNHRRFIAIEAIAGDLLRIQTETGDEIVQLLGVAAPRADEHW
                     GESARTYTANRLVGKSVALLLEPPQTRDVDGHLLAYVYPLDVDLINIDIVKDGMAYAD
                     RRVTAMLSPHVDLSESEARHKSRGLWKGLTYDAMPAWRRAWLAGQHRAQ"
     gene            complement(2684..3925)
                     /locus_tag="VLI90_10625"
     CDS             complement(2684..3925)
                     /locus_tag="VLI90_10625"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSV14706.1"
                     /translation="MALIVQKFGGTSVADAERIHRAARRAIAAKEKGNQVVVVVSAMG
                     HTTDELIDLAKQVCCHGGKDAVPPKREMDQLLVTGEQVTIALMAMAIHAQGHEAISFT
                     GGQIGLVTDTAFSKARIQSINKQRIFDQLNEGKIVIVAGFQGITPSGDFTTLGRGGSN
                     ATLVAVGAVLQADVCENYTDVDGIYTADPRICKNARKIDQISYDEMLELAGLGASVLQ
                     TRAVEFAKKYNVRIHVRNSQNDHEGTWIVAEKPNMEHIVVSGAALKKDLTRVTIKQVP
                     DRPGIAAKIFGDIAAGNIVVDDIIQNVMDDNTANISFTVEHGDLSDIKPVVDRLCKEL
                     GARTAPIYQGDLSKVSVVGVGMRVHTGVAQRMFKALADAKINIQNITTSEIRISCIIS
                     KEDGPKALQIVHDAFELAGTK"
BASE COUNT          687 a         1266 c         1268 g          855 t
ORIGIN      
        1 ggcgtatcgt tcgccgcttt gatgcgattc gtgtagctgt cccgcaggat gtgcactggc
       61 tcgcacgcct ttttaaagcc gaagtacgcg cctgtcggct caagttcgta cgtgtatgcc
      121 tggcagatca ggcatggcca cgccgattgc gtcatccaga ttaatccgcc gctgccctgg
      181 tggctttgcc aacattcgag catcgccttg gcactttcca ggttcagcag ctgcgccttg
      241 cggcagaaat cctcgacact gcttgcttcg ccatagctgt cgttcagccg cttcatgtac
      301 gccggcccgc gttcctggct caggtcgtgc tggccccaca ggtcgttgat cggccaaagt
      361 ttgtccggcg gcaaaaacgt ccgcagcgtt tccaccggtg gcaccgcgac gatgcccaac
      421 tcgctgtgca gcgtcatccc gcggtgctcg aaataccact cgggcgcgtg cacatcgtat
      481 ggaccaaatc ccgtcgcgcc gtcggcggcc gagtgcgaaa gatattttcg cgtcggatcc
      541 agctccttcg tcgccgcgtg cagctgctcg tccagtgatt tgggcggata gccttcgttc
      601 cgcccgcagt acagcgccag cgacggatga ttccgcaccc gccgaatctt gtcgcgggcg
      661 ttggtcatga acatcgcttc gtcgctgggg tcaggtccat cggcggggtt agcgagccag
      721 aagtcgtccc agatcaggat gccatattta tcgcaagcgt tgtagaagcc atcgcgcccg
      781 gtcatgccga cccagttgcg gatcatcgtg aagttcatct ccttgtgcag gcggacgcgc
      841 agctcgtagc cggcgtcgtc gcagcggagc atgccttcat ccatccccca gtttccgccg
      901 cggagcagca cgcgctggcc gttgcagtag atgaacagca cgttgtcgtg cgtctggtag
      961 gtgagcttgc gaatgccgaa ggggatttct ttggcgtcgc tggtgacgtt ggcgagatcg
     1021 acgcgcagct tcaggtgata gatcggctga tcgcccatgc cgttgggcca ccagagcttt
     1081 gggttgcgga tcaccagttg cgggcagttg gtcttgtcga acgtgatcgt cttggtttcc
     1141 gacgccgcga gcgagatggg cgaggagaat tcgatgttgt cgatcgcgcc gtgaacggtt
     1201 ccatcctgcg gttggttcga gtgattgacc agctcgacgc gtgccgttat ctcggcgaca
     1261 tccggcgagg gaagctcggt cgtaacgaac ggatcgacga tggacacgtc gccgctgctg
     1321 ctgagccaga cgtcgttcca gataccgacg ttgcgaccgg ggatcgtcgg cagccaattc
     1381 cacccgatgc tggagacgaa cgtcgggctg tcgagcccga gtatcccgcc gttgggcggc
     1441 cacttggcca gcgacttgtg ctttacctcg ccggggtgcg cgactttgtg cacgagcacc
     1501 gcgaggcaca ttttttcgcc cggcttcacc agcggcgtca cgtcgaaccg cccgcgcaga
     1561 aaagcgccgt cgatccggcc aacccgtgag ccgttgacga agatgtcggc cttccagttg
     1621 atgccgtcga agttcaacca gatgcggtgg ccggtgaact cttttggaac ggcgaacgtg
     1681 tcgcgatacc agaaatcgtt gtcggtgaag aaggcgtcat cgatttggta tcgctggtcg
     1741 gcgtagtacg gatcggggat tttcccggcg tcgagataac tgctcagcac cgtgccgggg
     1801 acggtggccg ggacccattg ccgatcgtcg aactcagccc gggcaatctg cttcggggat
     1861 gacaacacga gcgatgcgct ctgcaccttc caatttccgc cgacgagcaa caacgtgccg
     1921 ttcggttgca gctcgtgtgt tggtcgggat gttggtttgg gtatgacgga tctcggatta
     1981 gccgcttggg ccgaaatagc ggcggcgaat aagaacaagc cgatacaaat caacgtgaga
     2041 tgcacgccgc ctcccgatta ttgcgcgcgg tgctgccccg cgagccatgc tcgtcgccag
     2101 gcgggcatcg cgtcgtaagt caatcccttc cacagcccgc gcgacttgtg ccgggcttcg
     2161 ctttccgaca gatcaacgtg cgggctgagc atcgcggtca ctcgccgatc cgcgtacgcc
     2221 atgccgtcct tcacgatgtc gatgttgatc aggtctacgt ccaacggata gacgtacgcg
     2281 agcagatgcc catcgacgtc tcgcgtctgc ggcggttcga gcagcaacgc gactgatttt
     2341 ccaaccagac gattcgccgt gtaagttcgg gcgctctccc cccaatgctc atccgcgcgt
     2401 ggcgcggcaa cgccgagcaa ctgcacgatc tcatcgccag tctcggtctg aatccgcagc
     2461 aaatccccgg cgatcgcctc gatggcaatg aatcgccgat gattgaaccg agcgcagtcg
     2521 tccggcggcg cgcgaaggtg actgaaaccg gcgctgacca tcgcgaacgt aatcgtcaca
     2581 accgccgcga tgcgcacgat ccggcgccgg cgaagatacg accgaagccc cggatgtaca
     2641 tccggggctc cgctgtctcg cgcagcggtc gtcgtgctat cgctcatttc gtgcccgcca
     2701 gctcgaacgc atcatggacg atctgcaacg ccttcgggcc gtcttccttg ctgatgatgc
     2761 agctgatgcg gatctcgctg gtggtgatgt tctggatgtt gatcttcgcg tcggccaatg
     2821 ctttgaacat ccgctgggcg acgccggtgt ggacgcgcat gccgacgccg acaacgctga
     2881 ctttggacag atcgccctgg tagatcggcg cggtgcgagc gcccagttcc ttacacaggc
     2941 gatcgacgac cggcttgatg tccgacaggt cgccgtgttc gacggtgaag ctgatattag
     3001 ccgtgttgtc gtccatcacg ttctggatga tgtcgtcaac gacgatgttc cccgcggcga
     3061 tgtcgccaaa aatcttcgcg gcaatgcccg ggcggtccgg cacctgcttg atcgtgacgc
     3121 gcgtgaggtc ttttttcagc gctgcgccgg atacgacgat gtgttccatg ttcggcttct
     3181 ccgcgacgat ccacgtgccc tcgtgatcgt tctggctgtt gcgcacgtgg atgcgcacgt
     3241 tgtacttctt ggcgaattcg accgcgcgcg tttgcagcac cgacgcgccc aggccggcca
     3301 gctccaacat ctcgtcgtag ctgatctgat cgatcttgcg cgcgttcttg cagatgcgcg
     3361 gatcggcggt gtagatgcca tcgacgtcgg tgtagttctc gcacacgtcc gcctgcagca
     3421 ccgcgccgac ggccaccagc gtcgcgttgc tcccgccgcg gccaagcgtg gtgaaatcgc
     3481 ctgagggcgt gatgccctgg aagccggcga cgatgacgat cttgccttcg ttaagctgat
     3541 cgaagatgcg ctgcttattg atcgattgga tgcgggcttt gctaaacgcg gtgtcagtca
     3601 ccagcccgat ctgcccgccg gtgaagctga tcgcctcgtg gccctgcgcg tggatggcca
     3661 tcgccatcag cgcgatcgtg acctgctcgc cggtcaccag cagctggtcc atctcgcgct
     3721 tgggcggcac ggcgtccttg ccgccgtgac agcagacctg cttggccagg tcgatcagct
     3781 cgtcagtcgt gtggcccatc gcgctgacga cgacgacgac ttgattcccc ttctccttgg
     3841 ccgcgatcgc ccggcgcgcg gcgcggtgaa tgcgctccgc gtccgcgacg ctcgtcccgc
     3901 cgaacttttg cacgatcagg gccatggggg tgacgggcgg attatagaaa cgcgccgagg
     3961 ttgctcaacc agtgctcggc atgcgcggcg cacctggtaa ggcgccgccc atcctcctct
     4021 cccctcggga gaggcggggt gaggggatgt cgtcgtcgaa ggaacacagc gagcac
//
LOCUS       DATIMH010000615         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2745682, whole genome shotgun sequence.
ACCESSION   DATIMH010000615 DATIMH010000000
VERSION     DATIMH010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2745682"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..575
                     /locus_tag="VLI90_10630"
     CDS             303..575
                     /locus_tag="VLI90_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14707.1"
                     /translation="MVIEESKVMVARLKPELEQFIEQQVKAGRFASRDEMLEAGVARL
                     MLDPLRDELDEQDLAAIEESERQIAAGADLDWKQVSTQLRREYLGE"
     gene            1043..2362
                     /locus_tag="VLI90_10635"
     CDS             1043..2362
                     /locus_tag="VLI90_10635"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSV14708.1"
                     /translation="MPGRNVGIALLGCGVVGGGVASILLDQAELLRQRTGLSFHLRHV
                     VVRDAKKAERRAGLPITTDAGGAIADPAVDVVVELIGGTSTAAELIQSALKAGKPVVT
                     ANKSLLAARGPELFQLARKHNACIAFEASCGGGIPIINALTHGLIANRIDALVGIVNG
                     TCNFILTQMTRNGWSYDQALKEAQRLGFAEADPTLDVSGRDAAQKLAVLAGLAFNARV
                     EESNIHIEGIDRLQDQDIKLARELGYVIKLLAIGERVNGDRFTLRVHPTLVHQTDVLA
                     DVSGSFNAVSVYGHAVGHTLFYGRGAGQMPTASAVVSDIVDVALGVTPLAFKQLNIYP
                     DAVPAARVLPITELSSRYYLRLMAKDEPGVLAQVTKILGQHRISLSAIMQHEVNRGQF
                     VPIVITTHAAKEGAIQQSLKELDGLATITGPTVCLRIIDQPKEFAPA"
     gene            2598..>2787
                     /locus_tag="VLI90_10640"
     CDS             2598..>2787
                     /locus_tag="VLI90_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14709.1"
                     /translation="MSAAFFEALESRRMFATTPAPGNWSLQWADEFNSTPTTPAWVDT
                     LWGTTHFTGEQENYTPANV"
BASE COUNT          549 a          801 c          909 g          528 t
ORIGIN      
        1 ctgatccggc ggcagccgtc gtgaacttgt agaactccac cgggtccgag ccaaacgtga
       61 gatagtcgtc aaggttagat gacccacggc ccggtgctgt gtatctgtag tagatacaat
      121 cacttccttg ccgattaacg cgtccatcgg cgacgtcaca agcgactgta ggacgggccg
      181 ggcggccggg gtatgtccct gagcggggac gctcgccagc catcgggcga gggactgtag
      241 ccgcggttgc gtggggggaa agggtacttt ggggttacac ccatcgccaa ccggacgata
      301 caatggtcat cgaggagtcg aaggttatgg ttgcacgtct gaaaccggaa ctcgagcagt
      361 tcatcgaaca gcaggttaag gcgggccgct tcgcctcgcg cgatgaaatg ttggaagcgg
      421 gcgtagcgcg gttgatgctc gatccgctgc gcgatgagct ggacgaacag gacctggctg
      481 caatcgagga gtcggaacga caaatcgcag cgggagcgga tctggattgg aaacaggtgt
      541 ccactcagtt gcggcgtgag tacctcggcg agtagcgcgc gcacacccgc tacagcgacg
      601 tgcgcggagg gaactcatgc cgccgaaata catcgtgcgc atcacgcccg ccgcgttgaa
      661 ggacattcgc gggattttcg actacatcgc cactacgaag gagtcaccgc agaaccttcg
      721 aagatgatcc gcgcgttgct ggacgcgatc gatcgcctgg atatgttccc ccagcgatat
      781 gacatcgcgc ggactggatc gccccgcggc cgaaacgtca gatccatgcc ggtgaaaccg
      841 taccttgtga gataccgaat tgatgaaggg cgctcttatg tcttcattct ccacgtgcgc
      901 catggcgcgc agcgcggggc ttgagtgtcg ttcgcgaaag caccgtcgcg cgcacgaagg
      961 cggggccgct tcttgattca actgaagacc acatttagag tctcgaccgc atcacggtga
     1021 gttctctatt ggaacgtgga ttatgcccgg tcgaaacgta ggtatcgcat tgctcggttg
     1081 cggcgtcgtc ggcggcgggg tcgcttcaat cctgctcgat caagccgagc tgctgcggca
     1141 gcgaacaggc ttgtcctttc acctgcggca cgtcgtggtg cgcgacgcga agaaggcgga
     1201 gcggcgggcg ggattgccta tcaccaccga cgccggcggc gcgatcgcgg atcctgcggt
     1261 tgacgttgtt gtcgaattga tcggcgggac gagcaccgcg gcggaattga tccagtctgc
     1321 attgaaggcg gggaagcccg tcgtcacggc gaacaaaagt ctgctcgcgg cgcgcgggcc
     1381 ggaactgttt cagctggcgc ggaagcacaa cgcgtgcatc gcgttcgaag ccagctgcgg
     1441 cggcgggatc ccgatcatca atgcgctgac gcatggtttg atcgcgaatc gcatcgacgc
     1501 gctggtcggc atcgtcaacg gcacctgcaa cttcatcctc acgcagatga cgcgcaacgg
     1561 ctggtcgtac gatcaggcgc tcaaagaagc gcagcggctg ggatttgccg aggcggatcc
     1621 gacgctggat gtctcgggtc gcgatgcggc gcagaagttg gcggtgctgg cggggctggc
     1681 gttcaacgcg cgcgtggaag aatcgaacat ccacatcgaa ggcatcgatc ggctgcagga
     1741 tcaggacatc aagctggccc gcgagctggg ttacgtcatc aagctgctgg cgatcggcga
     1801 gcgcgtgaac ggcgatcgct ttacgctgcg cgtgcatccg acgctggtgc atcagacgga
     1861 tgtgctggcg gatgtcagcg gcagcttcaa cgcggtcagc gtgtatgggc atgcggttgg
     1921 gcacacgctc ttttatggtc gcggggccgg gcagatgccg acggccagcg cggtcgtttc
     1981 tgacattgtt gacgtggcgc tgggcgtgac gccgctggcg ttcaagcagt tgaacattta
     2041 tcccgacgcg gtgccggcgg cgcgggtgtt gccaatcacg gaattgagca gccgatatta
     2101 cctgcgcctg atggccaagg atgaaccggg cgtgctggcg caggtgacga agatcctggg
     2161 gcagcatcgg atcagcctgt cggccatcat gcagcacgag gtgaaccgcg ggcagttcgt
     2221 gccgatcgtc atcacgacgc acgcggccaa agagggggca atccagcaat cgctgaagga
     2281 gctggacggg ctggcgacga tcaccggccc caccgtgtgc ctgcgcatca tcgatcaacc
     2341 taaagagttc gcacccgcct gagcctgatt gcattttcga tgtcaattca ttgctagccg
     2401 ctgtatctga agcttgccta ccgctgtgcc cggcgcagca aaaaatcttt tcgcgacttg
     2461 aaaaaacttt tcgctgaaat cgctcctcgg cgcgttcagc cgcagttcag cccgtcttcg
     2521 cgccggtttt gctatgaacg cgctccgcgt ctctccgtac ccctcttaca ccctggattc
     2581 gcaacaagga gacaacgatg agtgcagcgt ttttcgaagc attggagtcc cgtcgaatgt
     2641 ttgcgacgac ccccgcgccg ggcaactgga gcctgcaatg ggcggatgag ttcaacagca
     2701 cccccaccac ccccgcgtgg gtggacacgc tgtggggcac gacgcacttc accggcgagc
     2761 aggagaatta cacaccggcg aatgtgg
//
LOCUS       DATIMH010000616         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3037795, whole genome shotgun sequence.
ACCESSION   DATIMH010000616 DATIMH010000000
VERSION     DATIMH010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3037795"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VLI90_10645"
     CDS             complement(<1..192)
                     /locus_tag="VLI90_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14710.1"
                     /translation="MNDEIRMTKRRDPRAFVIRHSDFIRGFGLRHSDFRTRITLTPAL
                     SRSTGRGGKSEARLPGSRGV"
     gene            complement(468..602)
                     /gene="rpmH"
                     /locus_tag="VLI90_10650"
     CDS             complement(468..602)
                     /gene="rpmH"
                     /locus_tag="VLI90_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HSV14711.1"
                     /translation="MHYPHKTSNIKRARKIGFRARMKTKNGRKTINAKRRVGRKVQVV
                     "
     gene            complement(640..>2915)
                     /locus_tag="VLI90_10655"
     CDS             complement(640..>2915)
                     /locus_tag="VLI90_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14712.1"
                     /translation="ATNLGDLKLSRAVKTLLEQDPKIGAVYALVEAKDGTIYAGTGPQ
                     GVLLQLKGDKVSTVAELDDGTSIFSMLVDAQGGVLLGTGGTKGRVYRIDKPGDKPRVL
                     FESDGVQYVWALVQTPDGNLYAATGPEGQLFEIKPDGSHSVVLDSDENNLLSMVGDGK
                     DTLYVGTEPNGLVYRINRKTHESFVLYDAAESEVGALALDGKGNLYAGTGEASDQPAT
                     AEDNAAKDQVGRPEGGPGGVPIQSVPPANPEPPALPNPNPGQPDPIPKNMMILADNKK
                     PMDDPDPGEPAKPTPPPGAPNPGEPPKPPKNPTPPAPGAKDNAPNAQTQQGINTTGTG
                     QPRAEGNAIYKIDPNGFVTEVFRQPVLVFSIVEHEGVLLVATGSEGLVYQVNPAAEET
                     VVIAKVDPKQVTCLLPTSDGRIVMGLANVGGLATMGSGYAGEGTYTSPVLDATQISKF
                     GKIELHGSLPQGTSLKVATRSGNVKEPTETSWSKWSEDQSATEFVQTHSPSARFFQYR
                     LMLGTKVDKTSPVVEDVDVAYLMPNLPPQIKGIKVTLGSKTAPPPPPPDPDAAPQPPQ
                     PNGRIQTIAWEANDPNNDALIYSLYYRSGSKAPWILLKDKLKEPIYEWDTRGVADGRY
                     DVKVVASDAAANPVGQGKTGSRVSDPILVDNTPPVIGDVKTKVAGTQVRVDVKAVDRT
                     SIVTAVDYAVDSAQDWQAASPSDTMFDSPEATANFTVSSLTPGPHQITVRATDTRGNQ
                     GFENVFITIDPPTAASK"
BASE COUNT          426 a          907 c          930 g          652 t
ORIGIN      
        1 aacaccgcgc gagccaggca gtcgcgcctc gctctttccc cctctcccgg tactccggga
       61 gagggctggg gtgagggtga ttcgagtgcg gaaatccgaa tgacgaagtc cgaatccccg
      121 aatgaaatcc gaatgacgaa tgacgaacgc gcgcggatcg cgccgtttgg tcattcggat
      181 ttcgtcattc attcggaatt cggatttcgt cattcttcat tcccaaagtc accctcaccc
      241 taaccctctc ccggcgtacc gggcgagggg accggagcgc gcactgccta aagcgtttgc
      301 cgcgccactc tgtgtgatat ctaagatgtc tggaaatgtt gaacgcgtga ctgaatgcgc
      361 gtgaacccgc tcggcgcgaa agcccaggct aagcttagcc tgggcttttg tttcacgatg
      421 cgataccgcg cccggggcgc acgtctgcgc gcagagcagc tcgtctttta gacgacctgc
      481 accttgcgtc caacgcggcg tttcgcgttg atcgttttgc ggccgttctt ggtcttcatg
      541 cgggcgcgga acccgatctt gcgggcgcgc ttgatgttgc tcgtcttgtg cgggtaatgc
      601 atggtcgctt ctcagttccg gcccttcttg ggcgctcgct catttcgacg ccgcggttgg
      661 cgggtcgatg gtgataaaca cgttttcgaa accctggttg ccgcgggtat cggtggctcg
      721 caccgtgatc tggtgcgggc caggcgtcag gctcgagacg gtgaaattcg ccgtcgcttc
      781 cggactgtcg aacatcgtat ccgatggcga agccgcctgc cagtcttgcg ccgaatcgac
      841 cgcgtagtcc accgccgtga cgatgctggt gcgatcgacc gccttcacgt ccacccgcac
      901 ctgggtgccg gccaccttgg tcttcacgtc gccgatcacc ggcggggtat tgtccaccag
      961 gatcgggtcg ctgacccggc tgccggtctt gccttggccc accgggttgg ccgcggcgtc
     1021 ggaggcgacg actttgacgt cataccggcc gtcggccacg ccgcgggtgt cccactcgta
     1081 gattggttct ttcagcttgt ccttcagcag gatccacggg gccttgctgc cgctgcgata
     1141 gtaaagcgaa tagatgagcg catcgttgtt gggatcgttg gcttcccagg cgatggtttg
     1201 aatgcgaccg ttcggttgcg gcggttgcgg ggcggcgtcc ggatcgggcg ggggtggcgg
     1261 tggggcggtc ttgctgccca gcgtcacctt gatccccttg atctgcggcg gcaggttcgg
     1321 catcaggtac gccacgtcca cgtcttccac cacgggcgag gtcttgtcca ccttcgtgcc
     1381 gagcatcaag cgatattgga aaaaccgcgc agatggtgaa tgtgtctgca cgaactcagt
     1441 ggctgactgg tcttcggacc atttcgacca gctggtttcg gtcggctctt tcacgttgcc
     1501 gctgcgcgtg gcgaccttca ggctcgtgcc ctgcggcaat gagccgtgca attcgatttt
     1561 accgaacttg ctgatctgtg tcgcgtccag cactgggctg gtgtaggtgc cttcgccggc
     1621 gtaacccgag cccatggtgg cgagcccgcc gacgttggcc agacccatca cgatgcggcc
     1681 gtcgctggtc ggcagcaggc aggtcacctg cttcggatcg accttggcga tgacaaccgt
     1741 ttcctcagca gcggggttca cttgatagac caacccttcg ctgccggtgg cgaccagcag
     1801 cacgccttcg tgttccacga tcgagaagac cagcactggc tggcggaaga cttcggtcac
     1861 gaagccgttg ggatcaatct tgtagatcgc gttgccctcg gcgcgaggct gtcccgtgcc
     1921 cgttgtgttg atgccttgct gcgtctgcgc gttcggcgcg ttgtccttcg cacccggggc
     1981 tggcggcgtc gggttcttcg gcggcttggg cggctcgccg ggattgggcg cgcccggcgg
     2041 cggggtgggc ttggcgggct cgcctggatc gggatcgtcc atgggttttt tgttgtccgc
     2101 caggatcatc atgttcttgg ggatcggatc gggctgcccc ggattaggat tgggcagcgc
     2161 gggcggttcg ggattggccg ggggtaccga ttgaatgggg acaccgccag gcccgccttc
     2221 aggccggcca acctggtcct tcgccgcgtt gtcttcggcg gtggcgggtt gatcgctcgc
     2281 ttcgcccgtg ccggcgtaga ggttcccttt tccatccagc gccagcgcgc cgacttcgct
     2341 ttcggcggcg tcgtacagca cgaacgattc atgcgttttg cgattaatgc gatacacgag
     2401 gccattgggc tcggtgccga cgtacagcgt gtccttcccg tccccgacca tgcttagcag
     2461 gttgttctcg tcgctgtcga gcacgacgct gtggctgccg tccggcttga tctcgaacag
     2521 ctgcccctcg gggcccgtcg cggcatatag atttccatcc ggcgtctgca cgagcgccca
     2581 gacatattga acgccgtccg attcgaacaa cacgcgcggc ttgtcgccag gcttgtcgat
     2641 gcgatatacc cggcccttgg tgccgccggt gccgagcagc acaccgcctt gtgcgtccac
     2701 cagcatgctg aagatgctgg tgccgtcgtc gagttccgcg acggtcgaaa ccttgtcccc
     2761 cttgagttgc agcaacacgc cctgcggccc ggtcccggcg tagatcgtgc cgtccttcgc
     2821 ttcgaccagc gcataaaccg cgccgatttt cggatcctgt tccagcagcg tcttgacggc
     2881 gcgcgaaagc ttcagatcgc ccaggttcgt cgcta
//
LOCUS       DATIMH010000617         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5635868, whole genome shotgun sequence.
ACCESSION   DATIMH010000617 DATIMH010000000
VERSION     DATIMH010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5635868"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..535)
                     /gene="rpsR"
                     /locus_tag="VLI90_10660"
     CDS             complement(329..535)
                     /gene="rpsR"
                     /locus_tag="VLI90_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSV14713.1"
                     /translation="MKVARTSAEGTVFIDYKDTESLKKMISGNGKILSRKRTGATAME
                     QRMLAKAIKRARYMALLPYVTAAM"
     gene            complement(914..1228)
                     /locus_tag="VLI90_10665"
     CDS             complement(914..1228)
                     /locus_tag="VLI90_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14714.1"
                     /translation="MSDQSKAPTLGEQDMAMYAAIAGRPPQIDPNAADIMDAPEPRVM
                     TRGWSPMVLSLYLRDVARQGALRLNRPEIAAWIRRQSDNAMKRLHESQTRIAHARNDR
                     AA"
     gene            complement(1388..1810)
                     /locus_tag="VLI90_10670"
     CDS             complement(1388..1810)
                     /locus_tag="VLI90_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14715.1"
                     /translation="MVAPKDGHGPGSAAACHAHASPAAMHIMSEVTRERPPRIERRRI
                     MRSTYRTQAWLQPADETGAIRSPLIHTRDLDEVGVGYIARQDLSALGDGILFLPSGNG
                     RPVRVPCRVRRSRELGGGWFEGLIEFINPQPMFATRRA"
     gene            2057..2857
                     /locus_tag="VLI90_10675"
     CDS             2057..2857
                     /locus_tag="VLI90_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSV14716.1"
                     /translation="MPAARLILYTGLGVSRELLQRQRGLPLALEMPAWIDPLRREPLE
                     AYARRMSLDVTRRVRKYADDPDSSRAAEEYGPSRVRSEPGVVGDDEMSQQDDVPLFLG
                     GVSFGAMVALEVARHVPGIAGVFLLSGALTGRAISPLLQPLARSMPYVPLALGLPILK
                     MSPLLYPLLGVSRTPDRRALDQVLPRASPALFRWGAWAAATWDPPALPAVPIHHIHGG
                     SDLIIPVQRVRADVIVKGAGHLLNLTHADAVNAFIMSRIQDTLKRGTA"
     gene            2862..3143
                     /locus_tag="VLI90_10680"
     CDS             2862..3143
                     /locus_tag="VLI90_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14717.1"
                     /translation="MANHRDAEITELVVVLDKRWEDKLQDAVQQLKSLGMEIYSADDD
                     NSVVEGCVESNKVHEIEKLDCVDYVRKVMTYIVDYPPGDPRDKDGPEDD"
BASE COUNT          561 a         1008 c         1034 g          608 t
ORIGIN      
        1 atccaacaaa ctgatgtgcc gctgctgcgc cgggatcagc tggttctgca tcagctgcgc
       61 gctgctcgcg tccacgtcgt cgatggcttc ctcgtaatcc ttcagcccat gctcttcgcc
      121 ttctttaagg gccttgagtg cgcccgggaa ggcatagcaa atcagaagtg gaagcagttg
      181 ccgctagcgg cttcgcaaac caaaaccgcg ccgctgatat tgcgaagccg gaagcggcgt
      241 tgcgggtggc aaaaaaacgc ccgaccctcc gcgggtcggg cgtgttggac actccctgtc
      301 cgaatttcgc ttggacgatc cagcgcgact acatcgcggc cgtcacgtac ggcagcagcg
      361 ccatgtagcg cgcccgcttg atcgccttgg cgagcatgcg ctgctccatc gcggtcgcgc
      421 cggtgcgctt gcgcgacagg atcttgccgt tgccgctgat catcttcttc agcgactcgg
      481 tgtccttgta atcgatgaac acggtccctt cggcgctggt ccgggcgacc ttcaccttgg
      541 ggcggttaaa cgtctgaaac ttttcggcct tttcccggcg ttcacgccca ccaccactgc
      601 cgccgcggcc accaaactgt cgagccattg ccaatctccg agcaaatacc tgattaaaat
      661 ttcgagccga gcattgtagc aacgccgccg gacatggcaa gggcgacgag aatgacaaaa
      721 acgaagtgcc gcgcgagggg ctacggacgg agaaaaaccc catttagccc ggcgtgaaca
      781 cacgccgggt ttacctgggt cttcgcggac ctttgattgg ggatggagcg gcctcgactt
      841 ccgagcgaaa tccggcgtgt gttcacgccg ggctaaatgg gggcgtcaac gtttgtgtgg
      901 gcgctgtgga aaatcacgcc gcgcgatcgt tccgcgcatg cgcgatgcgt gtctgcgatt
      961 cgtgtagccg cttcatcgca ttgtccgact gccggcggat ccatgcggcg atctccgggc
     1021 ggttcagccg cagcgcccct tgccgggcga cgtcgcgcag gtacaggctc agcaccattg
     1081 gcgaccagcc gcgcgtcatc acgcgcggct cgggggcgtc catgatgtcg gcggcattcg
     1141 gatcgatctg cggcggtcgg ccggcgatcg ccgcgtacat cgccatgtct tgctcgccca
     1201 acgtcggtgc tttgctctgg tcgctcatcg tggtgttctc cgtcgcgcct ttcaaactta
     1261 tccgcccgcg cgacgtcgaa tcttgagctt ttccagcgcc gtccgtcgct caataaacaa
     1321 agcccgggac acggcgagtg tcccgggctc aaaatcagat atgaccgaca tgtcgggcgg
     1381 cggtgtttca cgcgcgccgc gtcgcaaaca ttggctgtgg gttgatgaat tcgatcaagc
     1441 cttcgaacca cccgccgccc agttcgcgcg agcggcgcac gcggcacggc acgcgcaccg
     1501 gccggccgtt gcccgacggc aggaacagga tcccatcgcc cagcgcggac aaatcctgtc
     1561 gggcgatgta cccgacgccc acttcatcga gatcgcgcgt gtgaatcagc ggcgagcgga
     1621 tcgccccggt ttcgtcggcg ggctgaagcc aggcctgcgt ccgataagtc gaacgcatga
     1681 tccgccggcg ttcgatccgc ggcgggcgct cgcgcgtcac ttcgctcatg atgtgcatcg
     1741 ccgccggcga cgcgtgcgca tgacaggcgg cggcagaccc cggaccgtgt ccatctttcg
     1801 gcgcaaccac tctccacctc cacgtgaaat cgagttgctt gagttgagta acacagcaga
     1861 ggtaccccgc gaatcggtgt cgggcaaatc gcggtggaca gatggtgggc gagggaaaat
     1921 gtgcgcgatg cgcgggcgcg gattatcggc cgcttgcggc ttagcgtctg ttggaacgag
     1981 aagaaacaaa cgctaagccg cctgcggcgg gaatgccggt tccacttggc gcgcccgccg
     2041 gtcactatcg gacccgatgc cagcggcgcg cctgatcctc tacaccggcc tgggggtcag
     2101 ccgcgaattg ctgcagcgac agcgcgggtt gccgctggcc ctggagatgc ccgcgtggat
     2161 cgacccgctg cgacgcgagc cgctggaggc ttatgccagg cgcatgtcac tggacgtgac
     2221 acgccgcgtt cggaagtacg ctgacgaccc ggattcttct cgcgcggcgg aagaatacgg
     2281 gccgtcgcga gtacgctccg aacccggcgt ggtcggtgac gacgaaatgt ctcaacaaga
     2341 cgacgtccca ctcttcctcg gcggcgtttc gttcggcgcg atggtcgcgc tggaagtcgc
     2401 ccgccacgtg cccggcattg ccggtgtctt cctgctttcc ggcgcgctga ctgggcgcgc
     2461 tatctcgccg ctgcttcaac cgctggcgcg atcgatgccg tacgtcccgc tggcgctggg
     2521 cctgccgatc ctgaagatgt cgccgctgct gtatccgttg ctgggcgtct cgcgcacgcc
     2581 ggatcgtcgg gcactggacc aggtgctgcc tcgggcgtcg ccggcgctgt ttcgctgggg
     2641 ggcgtgggcg gccgccacgt gggacccgcc cgccctgccg gcggtgccga tccaccacat
     2701 ccatggcggc tccgatctga tcatccccgt gcaacgtgtg cgggcggacg ttatcgtaaa
     2761 gggggcgggt cacctgctga acctgactca cgcggacgcg gtcaacgcgt tcatcatgag
     2821 tcgtatccaa gacacgttga agagagggac ggcgtagacg aatggccaat catcgcgacg
     2881 cggaaatcac cgagttggtg gtcgtgctgg acaagcgttg ggaagacaag ctgcaggacg
     2941 cggtgcagca gctgaagtcg ctggggatgg agatctattc cgccgacgat gacaacagcg
     3001 tggtggaggg atgcgtggag tcaaacaagg tgcacgagat cgagaagctg gactgcgtgg
     3061 attacgtgcg gaaggtgatg acgtatatcg tcgattaccc gccgggcgac ccccgggaca
     3121 aagacggacc ggaagacgat tgattcaatt catcactttt agcggcgtgc ccgaagggcc
     3181 gcgtttttca tttttcagaa ccggtatcgt t
//
LOCUS       DATIMH010000618         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_729212, whole genome shotgun sequence.
ACCESSION   DATIMH010000618 DATIMH010000000
VERSION     DATIMH010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729212"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..>2253)
                     /locus_tag="VLI90_10685"
     CDS             complement(69..>2253)
                     /locus_tag="VLI90_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV14718.1"
                     /translation="PVHTHNGLRVLHTMKIPILDARGEPQFLLGISRDITEQQRVDEE
                     LRRHREHLEDMIQERTAELVIAKEQADAANQAKSDFLANMSHEIRTPMNAILGMSYLA
                     LQSRLDAQQLHYVQRVHGAAESLLGIINDILDFSKIEAGKLDIESIPFDLGDVMDNLA
                     NLVGLSAEEKGLEFLFAQPPQLPTNLVGDPSRLGQVLLNLGYNAVKFTDSGEVIVGVD
                     VIERSAHSVRLRFEVRDTGIGMTPEQQRRLFLPFSQADASTSRRYGGTGLGLAISGHL
                     VRLMGGELEVDSVHGIGSRFHFSLWLDLQPQQASVASRDASVFDRNRVLIVDDNPGAR
                     EILFAMCGALRMRPDTANDGEEALRKVALADAADDPYELVLLDWKMPGIDGIECAQRL
                     TRREVGRHAAPTILMLTAFSRDDARRCLEERQLAVGALLIKPVTPSTLFDACSVVLGM
                     PVHGQWRSTKREEAMAVHQQQLRGAQLLLVEDNEINREIALTLLGRAGIAAVTAADGQ
                     EALDMLERQHFDGVLMDCQMPVMDGYAATRAIRQQARWRDLPVIAMTANAMVEDRDRA
                     LAAGMNDHIAKPINVEEMFATLARWIRPSASQLRALTGFGAGDMRDAHLLNLEGIDSR
                     AGIAAMMGDSTLYVHLLRMFRDAQADFPERFRAARMRRDFDAAMRMAHDLKSVAGSLA
                     VLAVNRSASELEQACIDCGDDARIETLLQEVARPLGSVIAQLGAL"
BASE COUNT          393 a          742 c          704 g          414 t
ORIGIN      
        1 aacccgtatc gccgctaggc cagcctatcg atagaacgat ccggactgac ggatcagctg
       61 gcgtcgaatc acagcgcccc aagttgcgcg atgaccgagc cgagcggccg tgcgacttct
      121 tgcagcagtg tttcgatgcg cgcgtcgtcg ccgcaatcga tgcatgcctg ctcgagttcg
      181 gacgccgaac ggttgaccgc cagaaccgcg agcgagccgg caacgctctt caggtcgtgc
      241 gccatgcgca tggcagcgtc gaaatcccgg cgcatgcgcg cggctcggaa gcgctcggga
      301 aagtcggctt gggcatcgcg aaacatccgc agcaggtgca cgtacaaagt gctgtcgccc
      361 atcatcgcgg cgatgccggc gcggctgtcg atgccctcca ggttcaacag atgcgcgtcg
      421 cgcatgtcgc ctgcgccgaa ccctgtcaga gcgcggagct gcgacgctga cggtcggatc
      481 cagcgcgcga gcgtggcaaa catctcctcc acgttgatcg gcttggcgat gtggtcgttc
      541 attccggcgg cgagcgctcg gtcgcgatct tcgaccatcg cgttggcggt catcgcgatc
      601 accggcaggt cgcgccatcg ggcctgctgc cgtattgcgc gcgtggctgc atagccgtcc
      661 atcaccggca tctggcagtc catcaatacg ccgtcgaagt gctgtcgctc cagcatgtcc
      721 agcgcctcct ggccgtctgc ggccgtcact gcggcaatgc cagcgcgccc gagcaacgtc
      781 agggcgatct cacggttgat ctcgttgtct tcgacgagca gcaattgcgc tcctcggagc
      841 tgctgctgat gaacggccat ggcctcttcg cgcttcgtcg accgccactg gccatgcacg
      901 ggcatgccca gcaccacgct gcacgcatcg aacagcgtcg acggcgtgac cggcttgatc
      961 agcaacgcac cgacggcgag ctgacgctct tccagacacc gccgcgcatc gtcgcggctg
     1021 aacgctgtca gcatcaggat cgtgggggcc gcgtggcgcc ccacctcgcg ccgggtgagg
     1081 cgttgagcgc attcgattcc gtcgataccg ggcatcttcc agtcgagcaa aaccaactcg
     1141 tacggatcgt ccgcggcgtc ggcgagcgcc accttgcgca aggcctcctc gccatcgttc
     1201 gccgtgtccg gacgcatgcg cagcgcaccg cacatcgcga acagaatttc gcgggcgccg
     1261 ggattgtcgt ccacgatgag cacacggttg cggtcgaaca ccgaagcatc gcgcgacgcg
     1321 accgaagctt gctgaggctg caggtctaac cacaagctga agtggaagcg gctgcctatg
     1381 ccgtgaacgc tgtcgacctc caactcgcct cccatcaggc gcaccaggtg accgctgatc
     1441 gccaatccca gtcccgtgcc gccgtagcgg cgactcgtcg aggcgtccgc ctgcgagaac
     1501 ggcaggaaca gacggcgctg ctgttccggc gtcatgccga tgccggtgtc gcgaacctcg
     1561 aagcgcaatc gaaccgagtg ggccgagcgc tcgatgacgt cgacgcccac gatcacttcg
     1621 ccgctgtcgg tgaacttgac cgcgttgtag cccaggttga gcagcacctg accgaggcgc
     1681 gaaggatcgc ccacgaggtt cgtgggcaac tgcggcggct gggcaaacaa gaactccagc
     1741 cccttttcct ccgcactcag gcccaccagg ttggccaggt tgtccatgac gtcgcccagg
     1801 tcgaaaggga tcgactcgat gtccagcttg ccagcttcga tcttggagaa atcaaggatg
     1861 tcgttgatga tccccaacag cgattcggcc gccccgtgca ctctttgtac gtagtgcaac
     1921 tgttgagcgt ccagccgact ttgcaacgcg agatacgaca tgcccaatat cgcgttcatg
     1981 ggcgtgcgaa tctcgtggct catgttggca aggaagtcgc tcttcgcctg gttcgcggcg
     2041 tcggcctgtt ccttggcgat gaccagttcc gctgtgcgct cctgaatcat gtcctcgagg
     2101 tgctcgcggt ggcgcctcaa ctcctcgtcg acgcgttgtt gctccgtgat gtcgcgtgag
     2161 atgccgagaa ggaactgcgg ctcgccgcgt gcgtcgagaa tggggatctt catcgtgtgc
     2221 aacacgcgaa gaccgttgtg ggtgtgcacg ggc
//
LOCUS       DATIMH010000619         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1596021, whole genome shotgun sequence.
ACCESSION   DATIMH010000619 DATIMH010000000
VERSION     DATIMH010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596021"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VLI90_10690"
     CDS             <1..553
                     /locus_tag="VLI90_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSV14719.1"
                     /translation="IPDEIAILGVDNDELDCELASPPLSSVAVPWRRIGYDAAALLDR
                     VLDGEAIEPALTLVPPIGVVERQSTDSVAIADPEVAAAVRFIRQNAQGQIGVEDILRE
                     VPVGRRSLEQRFQAMLQRTPLEEIRRVRIERAKQLLATTDLPVRDIGKLSGFGQSTWF
                     SEAFLALVGESPARYRRRHQRAV"
     gene            763..1560
                     /locus_tag="VLI90_10695"
     CDS             763..1560
                     /locus_tag="VLI90_10695"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HSV14720.1"
                     /translation="MAKLTIHDLFEAKKTGRAFTEIRTSDLREAVACAQAGVEIIMCM
                     KEDLPLIRPAVPDVFIIAAHNVNLPSVCGADQAVGAAFELMNLGADAVYSAMSPRVVE
                     VMARESVPVVGHVGFVPYRSTWFGNKPRAVGKTADEARKVYQHAKAYQDAGAIGVEIE
                     IVPAKVAVEINRRLDKLILMSMGSGSGATIQYLFATDVLGTNPGHVPRHAKVYANIGE
                     EEARVQRLRVEAFKALSADVHSGTYPEEKHLVKMRDEEFDAFLKGLD"
     gene            2020..3048
                     /locus_tag="VLI90_10700"
     CDS             2020..3048
                     /locus_tag="VLI90_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV14721.1"
                     /translation="MKQIRWGIIGCGDVTEVKSGPGFQKATGSALVAVMRRDAAKAAD
                     YAKRHGVPRWYDDADRLIADPEVDAVYIATPPGSHEFYARKVCAAGKPCYVEKPMARN
                     APEAQRMVDAFAAARLPLFVAYYRRALPRFVKVREIVDGGAIGRILRISYRYADAQMI
                     HRETPVPWRLQAEHAGAGLFLDLGSHALDLLDRLLGPLQNVRGSAASVGRMFDVEDQV
                     QLTFTTLDGALGDATWSFTSDARADQYHIVGDRGELRFACFANEPITLAIEGRGMESF
                     DVPHPPHVAQPLIQTIVDELLGVTPPGSCPSTGESGLRSQRVMDQALQSYYGGREDGF
                     WNRPWAKT"
     gene            3216..>3466
                     /locus_tag="VLI90_10705"
     CDS             3216..>3466
                     /locus_tag="VLI90_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14722.1"
                     /translation="MSKLSVTSIALAVALVMGLSSLARAEDKAADYTGTWKWTATMRN
                     NSIDFTAKLKQDGDKVTGTIGGGNIPETEIKDGKVENGGL"
BASE COUNT          630 a         1082 c         1154 g          600 t
ORIGIN      
        1 catccccgac gaaatcgcga tcctcggcgt ggacaacgac gagctggatt gcgaactcgc
       61 cagcccgccg ctctctagcg tcgccgtgcc ctggcggcgc atcggttacg acgcggcggc
      121 gttgctggac cgcgtgctcg atggtgaagc gatcgaaccg gcgctcacgc ttgtcccgcc
      181 gattggcgtc gtcgaacgcc agtccaccga cagcgttgcc atcgccgacc ccgaagtcgc
      241 cgccgccgtc cgcttcatcc gccaaaatgc gcagggacag atcggcgtgg aagacatcct
      301 gcgcgaagtg ccggtcggcc ggcgctcgct ggagcaacgc ttccaggcga tgttgcagcg
      361 cacgccactg gaagagatcc gccgcgtgcg catcgagcgc gccaagcaac tgctggcgac
      421 gaccgatctg ccggtccgcg acatcggtaa attgagcggc ttcggccaat caacctggtt
      481 cagtgaagcg ttcctggccc tggtcggcga atcgcccgcg agataccgcc ggcggcatca
      541 acgcgctgta tagcacgggc ggccaccaga gcgagacggt ggcgcaggtg atcgaccaca
      601 accgcgtgcg cgcgcgccga gaacgtctcg cgtttagccg ccgcggcctc gcggcgatct
      661 tcgacgtccg ctccgcaagc ccgcggcggc taaaacggcg gtgggttttg ccgcgcgcga
      721 gcgcgtaccg tttcttcgtg ggttccgcta caaactgatg ccatggccaa gctgaccatt
      781 catgatctgt tcgaagccaa gaaaaccggc cgcgcgttca ccgagatccg caccagcgat
      841 cttcgcgaag cggtcgcctg cgcccaggcc ggcgtcgaga tcatcatgtg catgaaggaa
      901 gatctcccgc tgatccgccc ggcggtgccg gatgtgttca tcatcgcggc gcacaacgtc
      961 aacctgccgt cggtgtgcgg ggcggatcag gcggtggggg cggccttcga gttgatgaac
     1021 ctcggcgccg acgcggtcta ctcggcgatg agcccgcggg tggtcgaggt gatggcccgc
     1081 gaatcggtgc cggtggtcgg gcacgtcggg ttcgttccgt atcggtccac ctggttcggc
     1141 aacaagccgc gcgcggtggg caagacggcc gacgaagcac gcaaggtgta ccagcacgcc
     1201 aaggcctatc aggacgccgg cgcgatcggc gtcgagatcg aaatcgtccc cgcgaaggtg
     1261 gcagtggaaa ttaaccgccg gttggacaag ctgatcctga tgtcgatggg cagcggctcg
     1321 ggcgcgacca tccaatacct cttcgccacc gacgtgctgg gcacgaaccc cggccacgtc
     1381 ccgcggcacg cgaaggtcta tgccaacatc ggcgaagagg aagcgcgcgt gcaacgcctg
     1441 cgggttgaag ctttcaaagc cctcagcgcc gatgtccaca gcggcaccta tccggaagag
     1501 aagcacctcg tgaagatgcg ggatgaggag ttcgacgcgt tcttgaaagg cttggattga
     1561 tgggcatcgc tgacgtgaac agaggtcacg cgcggttctt tcccccctcc cggtactccg
     1621 ggagagggcc ggggtgaggg tgattttaga tttcgatagc cgttggcgct cgaaatcacc
     1681 ctcaccctaa ccctcctcta tgaagacccg tcgccggtca agagccgtgc acacgctttt
     1741 gaagcattcg gccgcccaat cttctattcg agctcggcca ccgttgcggc ctgcccatgg
     1801 gggttgtccg agacatccgt aacgactgcc ctatctttcg gcgagcaccg acaggtgccc
     1861 aatagggtaa cgagctcgtc atcacggacg cgggggccgc cctgcacgtt catgcacgtc
     1921 tcacctggac taaggcggcg ataatgccgc cggcttcgat tacattaagc gcgattcgga
     1981 atcaggccgg gcggcagcgc ccgcggccga cgtgcacgca tgaaacaaat ccgctggggc
     2041 atcatcggct gtggtgacgt gacggaagtc aaaagcggtc cgggttttca gaaggcgacg
     2101 ggcagcgcgt tggtcgcggt gatgcgccgc gacgccgcca aggcggcgga ttacgcgaag
     2161 cgccacggcg tgccgcggtg gtatgacgat gccgatcggc tgatcgccga tccggaagtg
     2221 gacgcggtct acatcgccac gccgccgggg tcgcatgagt tttatgcccg gaaggtgtgc
     2281 gccgccggca agccgtgtta tgtcgagaag ccgatggcgc ggaacgcgcc cgaagcgcag
     2341 cggatggtgg atgcattcgc ggcggcgcgg ctgccgctgt tcgtcgcgta ttaccgccgg
     2401 gcgctgccgc ggtttgtgaa ggtcagggaa atcgtcgacg gcggcgcgat cggccggatt
     2461 ttgcgcatct cctatcgcta cgccgacgct cagatgatcc atcgcgagac accggttccg
     2521 tggcggctgc aagccgagca cgctggggcg gggctgttcc tcgatctggg ttcacacgcg
     2581 ctggacctgc tcgaccggct gctcggtcct ctgcaaaacg tgcgcggcag cgccgcgagt
     2641 gtcggccgca tgtttgatgt cgaggatcag gtccagttga cgtttacgac actggacggc
     2701 gcgctcggcg acgccacgtg gtccttcacc agcgatgcgc gggcagatca gtaccacatc
     2761 gttggcgacc gcggcgagct gcgcttcgcg tgcttcgcca atgagccgat cacgctggcg
     2821 attgaagggc gtggaatgga atcgttcgac gtgccgcacc cgccgcatgt cgcccagccg
     2881 ttgatccaga cgatcgtgga cgaactgctc ggcgtcacgc cgcccggcag ctgcccgagc
     2941 accggcgagt ctggcctgcg cagccagcgg gtgatggatc aggcgctgca gtcgtactac
     3001 ggcggacgcg aagatgggtt ttggaatcga ccgtgggcaa aaacgtagca ggggcatctc
     3061 ggccgtgtcg agcggttcca ggcacgggcg cgacgctcgt gctacgaatt gggaagattt
     3121 ctatccgctc gcgcaactat agacggaccg gccgaggtgg cgatgagccg tcttgttgcc
     3181 gaacgttcac ctatctttct cgcttggagg atcgcatgtc aaagctcagc gtcacgtcga
     3241 ttgctctcgc agttgccttg gtcatgggac tttcgtcact cgcccgcgcg gaggacaagg
     3301 ccgcggatta caccggcacg tggaaatgga ccgccacgat gcgcaacaat agcatcgatt
     3361 tcaccgcgaa gctgaagcag gacggcgaca aggtcaccgg cacgatcggc ggcggaaata
     3421 ttccggagac cgagatcaag gacggtaagg ttgagaacgg cggact
//
LOCUS       DATIMH010000620         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1596196, whole genome shotgun sequence.
ACCESSION   DATIMH010000620 DATIMH010000000
VERSION     DATIMH010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596196"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2171)
                     /locus_tag="VLI90_10710"
     CDS             complement(<1..2171)
                     /locus_tag="VLI90_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14723.1"
                     /translation="MFQRRSLLSAWLLASAVLLPVSFAQNKRPLNHSDYDSWRSISGE
                     RLSRDGKFLIYGVFPQEGDGEIIIRNLVTGKEQREPGGMRPAPVQPTPGEEGPATPAR
                     GAILEFSADSKAAVFSTFPSKAESDKAKKDKKPAPKDAMVIVDLASGKATRIERVKRF
                     AMPDKASGLLVYLKEGPDSTTGGTSPTPATAGPGDQQGGRGGRGGGAAGAGRGARAEF
                     GSDLVVRTLADGTERMIPDVVEFTLTEDGKHVVYAVSARDAAKNGVFSVKPTATDAPV
                     ALLEGKGKYLKLTWDENQTQVVFLSDRDDTAAKQPKWKLYRWDRQANTASVLADADTP
                     GFRKEFIISDKGTISFSKDGTRVFFACAPPPPAPKDPAAEEVADDAKAVVDLWSYKDD
                     YIQPMQKVRAERDRNRTFTAAYLIPERKIVQLADAAMETVTPSESAQWIIGSDDREYR
                     PLADYDERYADAYLIDATTGARTLLAKKQRGTMTWSPNGRYLLYFDGKDWNTVSVPDG
                     KKTNLTAGISSKFFNEDTDTPSTPGPYGTGGWTKDGKNILLYDRYDIWRVGPDGAGVK
                     NVTSGYGRAHDLRLRYVRTEAENPRERWIDGTKPVMLQAENYKTFETGFFRASLDGGE
                     PRQLIMSPKYLTPPVKAKDADVYLLTAQTFSEFPDLVVTDGSFKELRKVSDANPQKAN
                     LLWGSSEVVQYKNSDGVPLTGALYKPANFDPAKKYPMMVYI"
BASE COUNT          430 a          740 c          723 g          518 t
ORIGIN      
        1 tagatgtaca ccatcatcgg gtatttcttc gccggatcga aattggcggg tttatagagc
       61 gcacccgtca gcggcacgcc atccgagttc ttatactgca caacttcgga gctgccccag
      121 agtaggttcg ccttctgcgg attcgcgtcc gagactttgc gaagttcctt gaagctgcca
      181 tccgtcacga ccagatcggg aaattcgctg aacgtctgcg ccgtcagcaa gtaaacatcg
      241 gcatccttgg cctttacggg cggagtcaga tatttgggcg acatgatgag ttgcctgggt
      301 tcgccgccat cgagtgaggc gcggaagaac cctgtctcaa acgtcttgta attctccgcc
      361 tggagcatta ccggtttcgt accgtcgatc cagcgctcgc gcggattctc ggcctcggtg
      421 cggacatagc gcaagcgcag atcgtgcgcg cggccgtatc cggaggtgac gttcttaacg
      481 cctgcgccat ccggacccac gcgccagata tcataacgat cgtagagcag gatgttcttc
      541 ccatccttgg tccagccgcc ggttccataa ggacccggcg tgctcggcgt gtccgtatct
      601 tcgttgaaga acttggaaga gatgccggca gtgagattgg tcttcttgcc atcgggcacg
      661 gagaccgtat tccaatcttt gccgtcgaag tagagcagat agcggccatt gggcgaccat
      721 gtcatggtgc cgcgctgctt cttggcgagc agggtgcgcg cgccggtggt ggcgtcgatc
      781 aggtacgcgt cggcgtagcg ctcatcatag tcggccagtg gacggtattc gcggtcgtca
      841 ctgccaatga tccactgcgc gctctcggac ggcgtgaccg tctccatcgc cgcgtcggca
      901 agctgcacaa tcttgcgctc cgggatgagg tacgccgcgg tgaacgtgcg attgcggtcg
      961 cgttccgcgc gcaccttctg catgggctga atgtaatcgt ccttataact ccataaatcc
     1021 acgacggcct ttgcgtcgtc cgcgacttcc tccgcggctg ggtccttggg cgcgggtggc
     1081 ggcggcgcgc aggcgaagaa gacgcgcgta ccgtccttgg aaaagctgat cgtgcccttg
     1141 tcgctgatga tgaattcctt gcgaaagccg ggcgtatcgg catcggccag cacactcgcg
     1201 gtgtttgcct ggcgatccca acgatacagt ttccatttcg gctgtttggc ggcggtatcg
     1261 tcgcgatcgc tcagaaatac gacctgcgtc tgattttcat cccaagtgag cttgagatat
     1321 ttgcccttgc cctcgagcag cgcgaccgga gcatcggtcg ccgtgggctt aacggagaac
     1381 acgccgttct tggccgcatc gcgcgcgctg acagcgtaca ccacgtgctt gccgtcttcg
     1441 gtaagcgtga actcgacgac atcagggatc atgcgctccg tcccgtcggc cagggtccgc
     1501 acgaccaggt cgctgccgaa ctcggcacgc gctccgcggc ccgcgccagc ggcgccgccg
     1561 ccccgaccgc cgcgtcctcc ctgctgatcg cccggtcctg ccgtagcagg agtggggctg
     1621 gtaccgccgg tcgtcgagtc cgggccttcc ttcaggtaca ccagcagtcc cgatgcctta
     1681 tcgggcatgg cgaaccgctt cacgcgttcg atccgcgtgg cctttccgga cgccaggtcg
     1741 acgatcacca tcgcgtcctt gggcgctggc tttttgtcct tcttcgcctt gtcgctttcc
     1801 gccttggaag gaaaggtgga gaagaccgcg gctttgctgt cggccgaaaa ctccagaatg
     1861 gcgccccgcg ccggtgtggc ggggccctcc tcgccaggtg tgggttgcac cggcgcggga
     1921 cgcatgccgc ccggctcgcg ctgttctttg cccgtcacca gattgcgaat gatgatttcc
     1981 ccgtcgcctt cctgagggaa gacgccatag atcaggaatt ttccgtcgcg cgaaaggcgt
     2041 tctccggaaa tcgagcgcca gctatcgtag tcgctatggt tgagggggcg cttgttttga
     2101 gcaaacgaca ccggaaggag gaccgcggaa gctagcagcc acgcggacag gagggaacgc
     2161 cgctgaaaca tgatctgcta ttgtaaacca gcccaccacc gcaggttgga ggcaaataca
     2221 ggccctgcgt taatcccgcc gacgtgatcg gcaataccaa ccggcgatcg gcgccgattc
     2281 gggccttcac cctgatcttg acagagatta gggtgatcag tataatttta gatactcaga
     2341 aggctgtcta atccctttgc ttccacaagc atagatatcc gcatcctgcc agcctgcctc
     2401 tgaatcgaat a
//
LOCUS       DATIMH010000621         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_729684, whole genome shotgun sequence.
ACCESSION   DATIMH010000621 DATIMH010000000
VERSION     DATIMH010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729684"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..967
                     /locus_tag="VLI90_10715"
     CDS             104..967
                     /locus_tag="VLI90_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HSV14724.1"
                     /translation="MGCGKESTAVNPTTVPAVKTVASLVPAVTDLIIGMGARDRLVAV
                     STYDRQRPDVASLPKVGDYQTVDWEQLQLIKPSILVIDIQADRRPAGFNERAESLHTQ
                     LVNITINRLSDVFAAIDQLGKALDLPQQATDAGRTLKTRLDAIEKRSADQNLPPVRTL
                     LVVGESGQSVVGPHTYLDDLLKAAGGVNVAAHLDQYPQVDREMLISLKPDVIVQLMPD
                     PSPQEREQAARAWRDLPQVPAVAAGRVYTIDSWYALLPGWHVADLAEQLAECLHPPTP
                     SSSPTTGRGAP"
     gene            910..1974
                     /locus_tag="VLI90_10720"
     CDS             910..1974
                     /locus_tag="VLI90_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSV14725.1"
                     /translation="MSAPADAIFITDDRPRRAVKRWSLARLLLTLAGCFAGWAVVALV
                     CLCVGSTGQLSWPTSDLLIIRREYVLLASLVGAALAAAGVVYQAILRNPLADPYLLGV
                     SSGASLAAYVWRFTWRGALLATLSSAMSQQLFAFAGGAIAIALVFALAMRRGRIEPIT
                     LLLVGVIVNAVNGSIFLLINTLKQNLTGGTGSAISFLVGGIQTNLTAWQEWSATIFIA
                     LGWIVLLYLSGELNVAVLSEAEAESLGVRIHRLRWIGLIAASMVTAAAVAISGPIGFV
                     GLVCPHLARLIVGTDQRRLLPVSTAIGASLLAIADAASRLLARADLAQTMLPVGVLTG
                     LLGGPFFLVLLWRSRRGAAM"
     gene            2034..>2753
                     /locus_tag="VLI90_10725"
     CDS             2034..>2753
                     /locus_tag="VLI90_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV14726.1"
                     /translation="MSLLKSSSVAFAYGDRSALRGVDLTLSAGEVVALLGPNGSGKST
                     LIKCLLGHLPAAGTIEWDGKPLAHWTRRALARRIAYLPQSPVAEPSQTVLDMLRLGRS
                     PYWSAFGLESPRDEAVVRSVSTRLGLDPLLNRPMDELSGGQRQRVFVGRCLVQEPAAM
                     LLDEPSTFLDLRHQVELCQLLRQLAKEQSIGVLMASHDLNLAAAYADRVFVLSDGTVA
                     ASGGIDALEPEMLSRVYGVKMV"
BASE COUNT          433 a          850 c          933 g          537 t
ORIGIN      
        1 tgcgcggttt gacagtggtt gacgccaaat ctaccatccg cgcgcgatgg gtcaacgacg
       61 tggatcgccc acaaaattca ttgtaccggc gatcgcgctt ctggtgggat gcggaaagga
      121 atcgaccgcc gtcaatccga cgaccgtgcc ggccgtcaaa accgtggcgt cgctcgttcc
      181 cgccgttaca gacctcatca tcggcatggg cgcgcgcgat cggttggtgg cggtcagcac
      241 gtacgatcgc cagcgaccgg acgtggcgtc gctgccgaaa gtgggcgact atcagacggt
      301 tgattgggag caattgcagc tgatcaagcc gtcgatcctg gtgatcgaca ttcaggctga
      361 tcgtcgtccc gcggggttca atgaacgggc cgagtcgctg cacacgcagc tggtgaatat
      421 cacgatcaat cggctgagcg atgtcttcgc ggcgatcgat caactgggta aagcgctcga
      481 tctgccgcag caggcgactg atgccggtcg cacactgaag acgcgactgg atgcgatcga
      541 gaagcgatct gcggatcaaa accttccgcc cgtgcgcacg ctgctggtgg ttggtgaatc
      601 ggggcagtcg gtcgtcgggc cgcacacgta cctggatgac ctgctgaagg ccgcgggggg
      661 tgtgaacgtc gcggcgcatc tggaccagta cccgcaggtt gaccgtgaga tgctgatctc
      721 gctcaagccg gacgtgatcg tccagctgat gcccgaccca tctccgcagg agcgcgagca
      781 ggcggcccgg gcgtggcgcg acctgccgca ggtgccggcg gtggcggcgg ggcgggtgta
      841 cacgattgac tcgtggtacg ctctgctgcc gggctggcac gtggccgatc tggctgagca
      901 gctcgccgaa tgtctgcacc cgccgacgcc atcttcatca ccgacgaccg gccgcggcgc
      961 gccgtgaaac gctggagtct tgcgaggctg ttgctgacgc tggccggctg tttcgctggc
     1021 tgggcggtcg tcgcgctcgt gtgcttgtgt gtgggttcga cggggcagct ctcctggccg
     1081 acgagcgatc tgctgatcat ccgccgcgag tacgtgctgc tggcgtcgct ggtcggcgcc
     1141 gccctcgccg ccgcgggcgt ggtctaccag gcgatcctgc gaaatccgct ggccgatcca
     1201 tatctgctcg gcgtcagcag cggggcgtcg ctggcggcgt acgtgtggcg cttcacgtgg
     1261 cgcggcgcat tgctggcgac gctatccagc gcgatgagcc agcaactgtt cgccttcgcc
     1321 ggtggcgcga tcgctattgc gctcgtgttt gcgctggcga tgcgccgcgg gcggatcgag
     1381 ccgatcacgc tgctgctggt cggcgtcatc gtgaacgccg tcaacggctc gatcttcctg
     1441 ctcatcaaca cgctcaaaca gaatctgacc ggcggcaccg gttcggccat cagcttcctc
     1501 gtcggcggca ttcaaacaaa tctgaccgcg tggcaggaat ggtcggcgac catcttcatc
     1561 gcgctcggct ggatcgtgct gctctatctt agcggcgaac tgaatgtcgc ggtgttgagc
     1621 gaagctgaag cggagtcgct gggcgtgcgg attcaccgct tgcgatggat cgggctgatc
     1681 gccgcgtcga tggtcacggc ggcggcggtg gcgattagcg ggccgatcgg ctttgtcgga
     1741 ttggtctgcc cgcatctggc gagattaatc gtcggcacag atcaacgaag actgctgccg
     1801 gtgtcgacgg cgatcggcgc gtcactgtta gccatcgcgg atgcggcgtc gcgcctgctc
     1861 gcgcgcgccg atctggcgca gacgatgttg ccggtcggcg tgttgacggg attgctcggt
     1921 ggtccgtttt tcctggtgct cttgtggaga tcgcgcagag gcgcagccat gtagcacggg
     1981 cgtctcgccc gtgcctaggc cattcgacac gggcgagacg cccgtgctac aaaatgtcgc
     2041 tcctcaagtc ttcatccgtc gccttcgcct atggcgatcg atctgcactt cgcggcgtcg
     2101 atctcacatt atctgccggt gaagtcgtcg cgctcctcgg ccccaacggc agcgggaaaa
     2161 gcaccctgat caaatgcctc ctcggccacc tgcccgcggc cggcacgatc gagtgggacg
     2221 gcaagcccct ggcgcattgg acgcggcgag cgctcgcccg gcggatcgca tatctcccgc
     2281 agtcgccggt cgccgaacca tcgcaaaccg tcctcgacat gcttcggctc ggccgctcgc
     2341 cgtactggtc cgcgttcggc ctcgaatcgc cgcgggatga agcggtggtg cgatcggtca
     2401 gcacgcgact tgggctcgat ccgcttctga accgtccgat ggacgaactg tccggcgggc
     2461 aacgtcagcg cgtgttcgtc ggccgatgcc tcgtgcagga accggcggcc atgctgctgg
     2521 atgagccgag cacgtttctc gacctgcggc accaggtcga gctgtgccag cttctaaggc
     2581 aattagcgaa ggaacagtca atcggcgtgc tgatggcatc gcacgacctg aacctcgcgg
     2641 cggcgtacgc ggatcgtgtg tttgtgttgt ccgacggaac cgtcgcggca tcgggtggca
     2701 ttgatgcgct ggaaccggaa atgctctcgc gcgtgtatgg cgtgaagatg gtc
//
LOCUS       DATIMH010000622         4436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3039615, whole genome shotgun sequence.
ACCESSION   DATIMH010000622 DATIMH010000000
VERSION     DATIMH010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3039615"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..2856
                     /locus_tag="VLI90_10730"
     CDS             169..2856
                     /locus_tag="VLI90_10730"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV14727.1"
                     /translation="MERSGRALVVVSLAVAAACARTLPAHAATPVVVINEAGFEGLEE
                     PSILYFPDTDTFQTDPINFKNHVDEGGSLFRDTLRNEYPSPVAPYAPGPGWWDGDGAD
                     NTRTDRQRSEPKGIVGLGHQQVEQTFEYSFDFRTDPTFTATSNFCHIFQLKATNGDDG
                     SPLATISLYKNGSAIQGRVDCFTDGTTGNNTEIIPTTFSYTAGQWSHFVIRITPCAQG
                     ETTGQIQLSVNGGAFKGVTNSAVDLTGSTDFRPKFGFYRGISTTNGVPIGDSWVEHRT
                     ITGYTGASNVLTWQGGQNNNTWDHNTTANFLNGASNSVFNTIDQVNFTGTTNTTINLA
                     GSLWPNFADVNSTANYTFQGAGAITGGTLRKDGSGTLTLATTNSYTGLTDVRAGTLLV
                     TGSIGNNSLASITGGTLKAGSTTALGSNSTIGTEINGGTLDINGFNLSTEPITAQGAG
                     VGGAGAIINTGAAQTSALNNVTLSSDTTIGGTGRWDIRGGSAMLSTSGNGYNLTKTGS
                     NQISLVGTSVDSALGDIAVNQGILAFQTSTTSMGDPSQTLTIASGATVSFFNTAAAMS
                     KIATLNGGTMWAESGTGTQNTFAGPITLGAAGGIFDAGSALTGGAANASALLNITGII
                     GGAGALTKNGPGTVTLSAVNTYTGATTVNAGTVALPAAAAINSSTITVNSGSTFNVNG
                     SLKTTAAVTVNGTANFSADTRTLATLSVGSGGLAAVASSASPFSPAVLQLSTLGFTNN
                     TSKLNLTNNELVANGSSTIALGLITNGNVYTTDTTPNIALGYIDVAGGKMEIRFTLKG
                     DTDLNGSVDVADLGALASNYGAAPGSGKTWAQGDFNRDGGVDVSDLGALATTYGSALA
                     GGPAASPTELIAAATAVPEPSTVMMVLPTMLLAQRRRRR"
     gene            2912..2994
                     /locus_tag="VLI90_10735"
     tRNA            2912..2994
                     /locus_tag="VLI90_10735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2946..2948,aa:Leu,seq:caa)
     gene            complement(3118..3480)
                     /locus_tag="VLI90_10740"
     CDS             complement(3118..3480)
                     /locus_tag="VLI90_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSV14728.1"
                     /translation="MTHHRIVGVSMILCSTCFEAIGQFTFKRAADTRPAGSGPIAAAL
                     SNYRWVLFGWFSFIIEGLFWSAALYFLDVTVAHPIGSIVFVVVAVLARLFLHERISPR
                     RWLGITLILAGTVLVAFN"
     gene            complement(3477..3947)
                     /locus_tag="VLI90_10745"
     CDS             complement(3477..3947)
                     /locus_tag="VLI90_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSV14729.1"
                     /translation="MPRLAILNEDRLPHQRVPLKVWTGLFIAVALDVPVQLMWKALMM
                     KYGEPSRAPRTHDLLHQVQWFAHQARTWALLGLFLCQFLNWMWVLGNADLSYAQPFTA
                     LSYVAVSGCAAIFFHEHLSALRIAGIALILFGVMLVGGSEHRTTAPTLSGGGAA"
     gene            complement(4064..>4436)
                     /locus_tag="VLI90_10750"
     CDS             complement(4064..>4436)
                     /locus_tag="VLI90_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14730.1"
                     /translation="GATAVLTDMPNHHFELRGPYVSEIARMDITAMLERNGGQAASDK
                     ELVKYRQAVAQVAADLDVPLAKYGDILDAQPPQQMVGVDGVHPSAAAHKLIAEALTPV
                     LTRACRTPALAKIAPVTGLSL"
BASE COUNT          885 a         1487 c         1351 g          713 t
ORIGIN      
        1 aggcgtcgca gctacctgaa cctagcgcag tcgccccggg atagatccca ccgcaatcga
       61 tttttacctt gagatatcgt cgcaagaggt cataatgcgt caaaggggcg gtccggctcg
      121 tcgatttacg cgcacgcgaa aaatccagcc gttgcagagg ctcccaggat ggaacggtca
      181 ggccgcgctc tcgttgtcgt ctcgctggcg gttgccgcgg cgtgtgcccg tacattgccc
      241 gcccatgcag ccacgcctgt agttgtcatc aacgaggcgg ggtttgaagg cttggaagag
      301 cccagcatcc tttattttcc cgacaccgac acgttccaga ccgatccgat caacttcaaa
      361 aaccacgtcg atgaaggcgg cagcttattc cgcgacacgc tgcgcaacga atacccgtcg
      421 cccgtggcgc cttacgcgcc ggggccaggg tggtgggacg gcgatggggc cgacaacacc
      481 cgcactgacc gtcagcgctc tgagcccaag ggcatcgtcg gcctcgggca ccagcaggtc
      541 gagcagacgt tcgaatattc attcgacttc cgcaccgatc ccaccttcac cgccaccagc
      601 aacttctgcc acatctttca gctgaaggcg accaacggcg acgacggttc gccgctggcg
      661 acgatctcgc tgtacaagaa cggctcggcc atccagggcc gggtcgattg cttcaccgat
      721 ggtaccactg gcaacaacac cgagatcatc ccgacgacgt tctcgtacac cgcggggcaa
      781 tggtcgcact tcgtcatccg catcacgccc tgcgcgcagg gcgagacgac ggggcagatc
      841 caactttccg tcaacggcgg ggcgttcaag ggcgtgacca actccgccgt cgatctcacc
      901 ggctcgaccg acttccgccc gaagttcggt ttctaccgcg gcatcagtac gaccaacggc
      961 gtgcccatcg gcgactcgtg ggtggaacat cgcacgatca ccggatacac cggcgcgtcg
     1021 aacgtgctca cttggcaggg tggtcagaac aacaacacct gggaccacaa cacgaccgcg
     1081 aactttctca acggcgcgag caactccgtc ttcaacacga tcgaccaggt caacttcacc
     1141 ggcacgacca acacgacgat caacctcgcc ggcagccttt ggccgaattt cgccgacgtg
     1201 aattcgacgg ccaactacac gttccaaggc gccggcgcga tcaccggcgg cacgctgcgc
     1261 aaggacggca gcggcacgct cacgctggcg acgaccaaca gctacaccgg attgaccgac
     1321 gtccgcgcgg gcacgctgct ggtcaccggg tcaatcggca acaactccct cgcatcgatc
     1381 accggcggga cgctgaaggc cggctcgacc acggcgcttg gttccaactc caccatcggc
     1441 accgagatca acggcgggac gctggacatc aacgggttta acctgtccac ggaaccaatc
     1501 accgcgcaag gggccggcgt cggtggcgcg ggcgcgatca tcaacaccgg cgccgcccaa
     1561 acaagtgcac tgaacaacgt cacgctgagt agcgacacca caatcggcgg caccggccgc
     1621 tgggatatcc gcggcggcag cgcgatgctt tctaccagcg gcaacgggta caacttgacc
     1681 aagaccggca gcaaccagat ctcgctcgtc ggcacgagcg tcgattcagc gctcggcgac
     1741 atcgcggtca accagggcat cctcgccttt caaaccagca cgacaagcat gggtgatccg
     1801 tcgcagacgc tgacgatcgc cagcggcgcg accgtcagct tcttcaacac tgccgcggcg
     1861 atgagcaaga tcgccacgct gaacggcggc acgatgtggg ccgaaagcgg cacgggcacg
     1921 cagaacacct ttgcaggccc gatcacgctc ggcgcagccg gcggcatctt tgatgccggc
     1981 agcgcgctca ccggcggcgc cgccaacgcc agcgcgttgc tcaacattac aggcatcatc
     2041 ggcggcgccg gcgcgctgac gaagaacggc cccggcaccg tgacgctctc cgccgtcaac
     2101 acctacaccg gcgcgaccac cgtcaacgcc ggtacggtcg cgctgcccgc cgcggcggcg
     2161 atcaacagct cgacgattac cgtcaacagc ggctcgacgt tcaacgtcaa tgggtcgctc
     2221 aagaccaccg ccgccgtgac cgtaaacggc acggcgaact tcagcgctga cacccgcacg
     2281 ctcgccacac tcagcgttgg gagcgggggc ctcgccgcag tcgcatcgtc ggcctcgccg
     2341 ttctcgccgg ctgtgcttca actctcgacg ctcggcttca ccaacaacac atcaaagttg
     2401 aacctgacga acaacgagct ggtcgcgaac ggctcgtcaa caatcgcgct cggcctgatc
     2461 accaacggca acgtctacac caccgacaca acaccaaaca tcgcgctcgg ctacatcgat
     2521 gtcgccggtg gaaagatgga aatccgcttt accctcaaag gcgacacaga tctgaacggc
     2581 tccgtcgatg tcgccgatct cggcgctctc gcgagcaact atggcgccgc ccccggcagc
     2641 ggcaagacct gggcccaagg cgacttcaac cgggacggcg gcgtggatgt ctctgacctg
     2701 ggcgctctag ccaccaccta cggctccgcc ctcgccggcg gccccgcggc ttctccaaca
     2761 gaactgatcg ccgcggccac cgccgtcccc gaaccttcaa ccgtcatgat ggtcttgccc
     2821 acgatgctgc tcgcccagcg ccgccgtcgt cgataactgt gcgggttgaa aaccgtgccg
     2881 cttccgcgat actgtttagc gccgaacccc cggcctcttg gcggaattgg cagacgcgat
     2941 ggattcaaaa tccatttccc aaaaggagta tcggttcaag tccgatagag gccatctgac
     3001 gcgagaaaac ccctgagcaa tcaggggttt ttgctttttg gattgcttgg tgcgccaaag
     3061 gatccgggtc gtcggagtac ctccgaaccc ggcgtggggg cggtccgcat cactcaatca
     3121 attgaatgcc accagcaccg tgccggcgag gatcagggtg atgcctagcc agcggcgggg
     3181 gctgatgcgt tcgtgcagga acaggcgcgc caggaccgca accaccacga agacgatgct
     3241 gccaatcggg tgggcgaccg tgacgtcgag gaagtacaac gcggcggacc agaagaggcc
     3301 ttcgatgatg aagctgaacc agccgaacag cacccagcgg tagttgctga gggcggcggc
     3361 gatcggaccg ctgccggcgg ggcgggtgtc ggcggcgcgc ttgaacgtga actggccaat
     3421 cgcttcgaag caggtgctgc agaggatcat cgacacgccg acgatgcggt ggtgggtcac
     3481 gcggcacctc cgccgctcaa ggtgggggcg gtggtgcgat gctcgctgcc gccgaccagc
     3541 atcacgccga acaggatcag cgcgatgccg gcgatgcgca gcgcggacag atgctcgtga
     3601 aagaagatcg cggcacagcc gctgacggca acgtacgata gcgcggtgaa cggctgggcg
     3661 taactgaggt ccgcgttgcc gagcacccac atccagttga ggaactggca gaggaacagc
     3721 ccgagcagcg cccacgtgcg ggcctggtgg gcgaaccatt ggacctggtg cagcaagtcg
     3781 tgcgtgcgcg gggcgcgtga cggctcgccg tacttcatca tgagcgcctt ccacatcagt
     3841 tgcaccggca cgtccagcgc aacggcgatg aagaggccgg tccaaacttt cagcggaacg
     3901 cgctgatgcg ggaggcgatc ttcattcaga atagctaatc gtggcacggg ctcgtggccc
     3961 gtgcgcgagg cttgggaatc ttccagggtc aaaacggttc tcgcgcgtcc gtagcgtctc
     4021 gcacgggccg gaggcccgtg tcacggtggg attgtacagt cgcctacagt gatagtccgg
     4081 tcaccggcgc gatcttcgcc aacgccggtg tgcggcaggc gcgggtcagc acgggcgtga
     4141 gcgcctcggc gatcagcttg tgagcggcag cgcttggatg cacgccatca acgccgacca
     4201 tttgctgcgg cggttgcgcg tccaagatgt cgccatactt cgccagcggc acgtcgagat
     4261 cggcggcgac ttgggcgacg gcttggcgat atttcaccag ttctttgtcg ctcgccgcct
     4321 ggccaccgtt gcgttccagc atggcggtga tgtccattct cgcgatctcc gacacgtacg
     4381 gcccgcgcag ctcgaagtga tggttcggca tgtccgtcag caccgccgtc gcgccg
//
LOCUS       DATIMH010000623         3885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_150512, whole genome shotgun sequence.
ACCESSION   DATIMH010000623 DATIMH010000000
VERSION     DATIMH010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150512"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..585)
                     /locus_tag="VLI90_10755"
     CDS             complement(169..585)
                     /locus_tag="VLI90_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14731.1"
                     /translation="MPPIYLSIFAVALTAVAAVEELRRRGRTHCQLKQLAVEGHLHFS
                     AHDQLRLADRVAAVFPVPGAAHVRVVDLLYGSQGGSHRYVFTAQYTIGAVRSKKRVRR
                     AATLSEPRDRAPGQCCQVRLATGTLPIVEQYRELLS"
     gene            complement(598..1860)
                     /locus_tag="VLI90_10760"
     CDS             complement(598..1860)
                     /locus_tag="VLI90_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14732.1"
                     /translation="MVRQRQRHWLVGLIGVGLLATATSMLPAKPGIVRTKDGTVYRGD
                     VTEDEKFVTIKDRGIATRIDKRNIPAGAIQYTASIDDQFNERHAALAPNDIKGRMDLA
                     NWATQNERPDLAVTALEEATKIEPTNRDAALALDTAQRQVELDQRKKGAGAATPPPPP
                     PGPGAKPGTTPPPPATRGVASQPAAAAPPGPGGKQPPMEHRLLNADEINTIRQKEMLA
                     GDPKVIVQFKNDVVKKYLATGARNTEGFRKESQVEQAIEILDHGDPKLANDVRIMTDP
                     SPIVDFKQKVYPVVATSCASTACHGGTKAGNFGLFPGQSEPALYTNFYILQTYAKTIN
                     GTQYLMMDRQLPERSLFLQYAVPASESEVSHPQAPNFRPRFRTKTDAGYKAVLDFLKS
                     TLNPISPNYGIKVAPSLPAAQPAADAPK"
     gene            2023..2343
                     /locus_tag="VLI90_10765"
     CDS             2023..2343
                     /locus_tag="VLI90_10765"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HSV14733.1"
                     /translation="MSSGDEFVSEPITPEAASFDTSAMSRGEPGIPRRFTWRAQTYEV
                     AALLSTWKTSTRERGELYLRRHWFEIQTKDDRRMTLYCERQKKMAKKPKQRWWVYSVS
                     RASM"
     gene            complement(2614..3366)
                     /locus_tag="VLI90_10770"
     CDS             complement(2614..3366)
                     /locus_tag="VLI90_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSV14734.1"
                     /translation="MTANVALFISCLTDQFYPRVGVAVTKVLEHLGCDVEFPQAQTCC
                     GQPFFNNGFHDDARALARKFVQDFEAYEYIVTPSGSCCAMVREHFPQLLKDDHAYDRG
                     MRYVASRTFEFIEFLDKVLHVDLSQLSLPEPRTITYHYTCHLRGLGVTDQPVRLLRQI
                     GNVEFRPLEKTDQCCGFGGTFAVKYPAISGAIVEDKIKHIAATKAQVTVCNDAGCTMN
                     IAGMCHRAGVETRVRHMAELLAEAMGIEIDRW"
     gene            complement(3363..>3885)
                     /locus_tag="VLI90_10775"
     CDS             complement(3363..>3885)
                     /locus_tag="VLI90_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14735.1"
                     /translation="SPMIDNLEGDAVAWDRVERGLPVLCAGDGLGLWVSTHRDDCGKF
                     FAYAAGNAKTFGQSYNATRDEHLTWGEYYQQVAAALGREARALFMPAAWIVKHNPQRF
                     GLLREITAFHGAYDSSKAKRDVPEFKCEIGLIDGARATFEDARRRGGWKDSAADALYQ
                     SMVDQALSMDGNA"
BASE COUNT          645 a         1285 c         1225 g          730 t
ORIGIN      
        1 ttcggtcccc tcgcccatgt actcatggga gagggttagg gtgagggtga tttagaattt
       61 taaatgtgtc acaaaaacag aatcaccctc accttgaccc tctcccggag taccgggcga
      121 ggggacaaga acgagatcgg aagccgttga aagcctcgcg cgtcgaactc agctcaacaa
      181 ctcccgatac tgctccacga tcggcaacgt ccccgtcgcc aggcggactt ggcagcactg
      241 ccccggcgcg cgatcgcgcg gctcgcttag cgtcgccgcc cggcgcaccc gcttcttcga
      301 gcgtacagct ccgatcgtgt attgcgcggt aaagacgtaa cggtgcgacc cgccctggct
      361 gccgtatagc agatcaacca cccgcacgtg cgccgccccc ggcaccggga acaccgcggc
      421 aacgcgatcc gccaggcgca gctggtcgtg cgcgctgaag tgcagatgcc cctcgaccgc
      481 cagctgcttc agctggcagt gcgtgcgccc gcgccgccga agctcctcga cggcggcaac
      541 cgccgtcagc gcaaccgcga agatcgacag gtaaatcggc ggcacggtgt gcgcggctta
      601 cttgggcgcg tcggccgccg gttgcgcggc cggcaggctg ggcgcgacct tgatcccgta
      661 gttcggcgag atcgggttca gcgtgctctt caggaaatcc agcaccgcct tgtaccccgc
      721 gtcggtcttc gtgcgaaacc gcgggcggaa gttcggcgct tgcgggtgcg acacttccga
      781 ttcgctggcc ggcaccgcgt attgcaggaa cagcgaccgc tccggcagct ggcggtccat
      841 catgaggtac tgcgtgccgt tgatcgtctt ggcgtaggtc tgcaggatgt agaagttcgt
      901 gtacagcgcc ggctcgcttt ggccggggaa gagcccgaaa tttcccgcct tggtcccgcc
      961 gtggcatgcg gtcgatgcgc aactggtcgc gacgacggga taaaccttct gcttgaagtc
     1021 cacgatcggc gacggatcag tcatgatccg cacgtcgttg gccagcttcg gatcgccgtg
     1081 gtccaggatc tcgatcgcct gctcgacctg cgactctttt cggaagccct cggtgttccg
     1141 cgcgccggtc gcgagatatt tcttcacgac gtcgttcttg aactgcacga tcaccttcgg
     1201 atcgccggcg agcatttcct tctgccggat cgtgttgatc tcgtcggcgt tgagcaggcg
     1261 atgttccatc ggcggctgtt tgcctccggg tccaggcggc gccgcggccg cgggttgcga
     1321 agcaacgccg cgcgtcgccg gtggtggtgg cgtggtccca ggtttggcgc ccggccccgg
     1381 cggtggtggt ggcggcgttg ccgcgccagc ccctttcttg cgctggtcca gctcgacctg
     1441 ccgctgcgcc gtatccagcg ccaacgccgc gtcgcgattg gtcggttcga tcttcgtcgc
     1501 ctcttccagg gccgttaccg ccaggtccgg ccgctcgttt tgcgtggccc agttggccag
     1561 atccatccgc cctttgatgt cattgggcgc gagggcggcg tgacgctcgt tgaactgatc
     1621 gtcgatgctg gccgtgtatt gaatcgcccc ggcgggaatg tttcgcttgt cgatgcgggt
     1681 ggcgatgccg cggtctttga tggtgacgaa cttctcgtcc tcggtaacgt cgccgcgata
     1741 aacggtgccg tccttcgtgc gcacgatgcc gggcttggcc ggcagcatgg acgttgcggt
     1801 tgccagcaac ccgaccccaa tgaggcccac gagccagtga cgctgccgct gccgcaccat
     1861 agatgtctcc tcacgcgatg atcgcaggcg cggcgcgcca tgatggcccg ctcgcgctgc
     1921 cgtcgcctta ctcgtggcat cttaagtaga tggggaaaac gtggcaactt tgacaatatc
     1981 gcacgccagc gcgggtacct gccgtgctac aacaggcgca cgatgtccag cggcgacgag
     2041 ttcgtgagcg agccgatcac acccgaagcc gccagcttcg acaccagcgc gatgtcccgc
     2101 ggtgaacccg gcatcccgcg gcgattcacc tggcgcgcgc aaacgtacga ggtggccgct
     2161 ctgttgagca cctggaaaac cagcacccgc gaacgcgggg agctttacct gcggcggcac
     2221 tggtttgaga tccagacgaa agacgatcgg cgcatgacgc tgtactgcga gcggcagaaa
     2281 aaaatggcga aaaagcctaa gcagcggtgg tgggtgtatt cggtgtcgcg ggcgtcaatg
     2341 tagcgctgat ggggacatca tcctccagcg caggcgtact cgctggagtc gaaggatctt
     2401 gcgcggagtc tgtgacccgt cactcgcgcg atcgggaggg cagtggcttg tcccttcgcg
     2461 gaagcccggc gatgaatccg cgaaggggca agcgacttcc aaaccatccg cgtccaacga
     2521 gagttcgaat acttcgaggc aagatccttc gactgcgcac gagtacgtgc ttcgctcagg
     2581 atgacgcgcg cacacacgca cgcgcacgcc acatcaccac cggtcgatct cgatccccat
     2641 cgcctccgcc aatagctccg ccatatggcg cacgcgcgtc tccacccccg cgcggtggca
     2701 catgccggcg atgttcatcg tgcaccccgc gtcgttgcag accgtcacct gcgccttggt
     2761 cgcggcgatg tgctttattt tatcttctac gatcgcgcca ctgatcgccg gatacttcac
     2821 cgcgaacgtc ccgccgaagc cgcagcactg gtcggtcttt tccagcgggc ggaattcgac
     2881 gttgccgatc tgccgcaaca ggcgcaccgg ttggtccgtt acgcccagcc cgcgcaggtg
     2941 gcaggtgtag tgatatgtga tcgtccgcgg ctccggcagc gacagctgcg acaggtccac
     3001 gtgcagcacc ttgtcgagaa actcgatgaa ctcgaaagtg cgcgacgcga cgtatcgcat
     3061 gccgcggtcg tacgcgtggt cgtccttcaa cagctggggg aaatgctccc gcaccatcgc
     3121 gcagcagctg ccgctgggcg tgacgatgta ttcgtatgcc tcgaaatcct gtacgaactt
     3181 tcgcgccagc gcccgggcgt catcgtggaa tccgttgttg aagaacggtt ggccgcagca
     3241 ggtttgcgct tgcgggaact cgacgtcgca gccgaggtgc tcgagcacct tggtgacggc
     3301 cacgccgacc cgcgggtaga actggtccgt caggcagctg atgaacagcg cgacgttcgc
     3361 ggtcatgcgt tgccgtccat cgacagtgcc tgatcgacca tcgattgata aagggcatct
     3421 gccgcgctgt ccttccaccc cccccgccgg cgggcatctt cgaacgttgc gcgggcgccg
     3481 tcgatcaggc cgatctcgca cttgaactcc ggcacgtcgc gcttggcttt ggacgaatcg
     3541 tacgccccgt gaaacgcggt gatttcccgc agcaggccga aacgctgcgg gttgtgcttg
     3601 acgatccacg ccgccggcat gaacagcgcg cgcgcctcgc ggcccagcgc cgccgccacc
     3661 tgctggtaat actcgcccca cgtgagatgc tcgtcgcgcg tcgcgttgta cgactgccca
     3721 aacgtcttgg cgttccccgc ggcgtaggcg aagaacttgc cgcagtcatc gcgatgcgtc
     3781 gagacccaca gccccagccc atcgcccgcg cacagcaccg gcaggccgcg ctcgacgcgg
     3841 tcccacgcca ccgcgtcgcc ttccaggttg tcgatcatcg ggctg
//
LOCUS       DATIMH010000624         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_150986, whole genome shotgun sequence.
ACCESSION   DATIMH010000624 DATIMH010000000
VERSION     DATIMH010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150986"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..583)
                     /locus_tag="VLI90_10780"
     CDS             complement(41..583)
                     /locus_tag="VLI90_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14736.1"
                     /translation="MSTNEPKSNAPASDAPTDAEKDQAKHADEADFLTRQADEAKAAI
                     ASALADAKAALAEGIDPREWTRRYPFVALGSAVAAGFVAAVLTIPSKEQQELKKLEKI
                     RRAMHPEVDEVKSKTKAAVADAADEAKAESHKHSFWSTILREAVQMIRPILISAMTAG
                     LGAKAAQNADGRPPDRAPGS"
     gene            complement(543..1055)
                     /locus_tag="VLI90_10785"
     CDS             complement(543..1055)
                     /locus_tag="VLI90_10785"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HSV14737.1"
                     /translation="MVADGNKPGFSGYAAERETAAAEDDDPTPASAFGEMGERISEMR
                     DYAAYYVKARVDKIKAALRNILVMAALGIVGLIAGGALVVTAVVLLCTGIAQGLTALF
                     GGHAWAGNLVTAVLLLGMIGIVAYVALTKITKTSKERMVHAYEQLKREQRADHGTDVH
                     ERAEEQRAGV"
     gene            complement(1201..1533)
                     /locus_tag="VLI90_10790"
     CDS             complement(1201..1533)
                     /locus_tag="VLI90_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14738.1"
                     /translation="MSRMNDPSSQGNMGGGSAGGGGGAGATDQLRDKASEVGQNLRDM
                     GSQARDAATEKYNQLRDQASQYYTQGREAAQEWEQNLESYIHEQPMKAVLMAAGVGLL
                     LGLLWKRS"
BASE COUNT          364 a          718 c          626 g          420 t
ORIGIN      
        1 taccgccccc gcctataaag gattgatcag ccaattcaaa ttacgatccc ggcgcgcgat
       61 ccggcggtcg tccatccgca ttctgcgccg ccttcgcgcc taaccccgcg gtcatcgcgc
      121 tgatcaggat ggggcggatc atctgcaccg cttcgcgcag gatcgtggac cagaacgaat
      181 gcttgtgcga ctccgccttc gcttcatccg cggcatcggc gacggcggct ttagtcttgc
      241 tcttcacctc gtccacttcc gggtgcatcg cccggcggat cttttccagc ttcttcagct
      301 cctgctgttc cttcgagggg atcgtcaaca ccgccgccac gaaaccggcg gcgacggcgg
      361 agccgagtgc gacgaacggg tatcgccgcg tccattcgcg cgggtcgatc ccttcggcca
      421 gcgctgcctt ggcgtcggcc agcgccgatg cgatggcggc cttggcctcg tcggcctgcc
      481 tggtcaggaa gtccgcctcg tcggcgtgct tggcctgatc cttttccgcg tcggttggtg
      541 catcagacgc cggcgcgttg ctcttcggct cgttcgtgga catcggtgcc gtggtcggcg
      601 cgctgctcgc gcttgagctg ttcatacgcg tggaccatcc tttccttgga agtcttggtg
      661 atcttcgtca gcgcgacgta cgcgacgatg ccgatcatgc ccagcagcag gaccgcggtg
      721 acgaggttgc ccgcccacgc gtgaccgccg aacagcgcag tcaatccctg cgcgatgccg
      781 gtgcacagga gcacgaccgc cgtcacgacc agcgccccgc cggcgatcaa accgacgatg
      841 ccgagcgcgg ccatcacgag gatgttgcgc agcgccgcct tgatcttatc gacgcgtgcc
      901 ttgacgtagt acgcggcgta atcgcgcatc tcgctgatgc gctcgcccat ctcgccaaag
      961 gcgctggcgg gcgtgggatc gtcgtcctcg gccgccgcgg tttcgcgctc ggcggcgtac
     1021 ccagagaaac ccggcttgtt accgtcggct accatgacgt gtccctcgaa cggtcaatgg
     1081 gaaaacggaa aacggccgaa ggagacgcac gcagcgattg ttcgcgagcg cggacgtcgc
     1141 cccttcacat tttcccttta ccattttcca ttaaccatga gttgcgcgga agccgcgcaa
     1201 ctagcttcgc ttccacagca aaccaagcag cagcccgacg ccggcggcca tcagcacggc
     1261 cttcattggt tgctcgtgga tgtagctctc caggttctgt tcccattcct gggcagcctc
     1321 gcggccctgc gtgtaatact ggctggcctg gtcgcgcagc tggttgtact tctcggtcgc
     1381 ggcatcgcgg gcttgcgagc ccatgtcgcg caaattctgt ccgacttccg aggccttgtc
     1441 gcgcagctgg tcagtcgcgc cggcgccacc gccgccgcca gcactgccgc cacccatgtt
     1501 gccctgggac gatggatcat tcatacgaga catagcgatc tccttcgtaa agacgaagtt
     1561 cagcgaatcg aattgtaccg tgcgctgcgc ttcgccggtg aaactttaag ccgggaacca
     1621 cacacacaca tgcttcagcc ggctgcccgc aactcaggtg ccacatcgct tatagaccga
     1681 tggagccagc catgcagcgc gtaaaatggg gacgggtgcg agtcgcgcca agagcaccag
     1741 gacgccaaga cgcagagaag cactcgacgt ttgacacgcc tccgccactc tgcggctccg
     1801 cgattcgatc cttttatttc ttcccttggc gctcttggcg tcttggcgtc ttggcgaaac
     1861 agcggcgtac gaaaaatcga ccgacacaga acccgcgtca tgcccccagc aaaggtgaca
     1921 gacccgacac ttcttatgtc gcggtagcgg cagcgcgtaa aataaatggc gcaacggggt
     1981 catcgcgagg gacttcatat cggccgattg tccccttcct catcgcagga gccgcacgtg
     2041 ggcattcgtg agaagctgaa cgaccggccc ggtctcaacg ccgtcgccga ctttctcggg
     2101 gtcagccggc acaccgtcgt ccgctggc
//
LOCUS       DATIMH010000625         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1597914, whole genome shotgun sequence.
ACCESSION   DATIMH010000625 DATIMH010000000
VERSION     DATIMH010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597914"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1526
                     /locus_tag="VLI90_10795"
     CDS             <1..>1526
                     /locus_tag="VLI90_10795"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSV14739.1"
                     /translation="PSGVAILQDLKRFANLGSVLMVAAHPDDENTQLITYLARGRGYR
                     TAYLSVTRGDGGQNVLGPEFGEELGVIRTQELLSARRLDGGRQFFTRAIDFGFSKDFQ
                     ETLGIWDRKQVLGDVVRVIRIFRPDVIVTRFSPKPGNTHGHHTASAVLALDAFKIAGD
                     PKAYPEQLVGDMKPWQPKRIFMNGGGPRGNDAAGANPSAIRIDDGGTDPVLGESFGGI
                     ARRSRAMHKTQGFGNFAIGAGGGGPHQEAFVLLDGEPATADILDGVDTTWNRVPSGGA
                     AIATAVDAAIKSFSVDNPAASAPALLDIRGKLAGGAADDIVVNEKRRQLDRIIADCIG
                     LSVQTTVPRAEYVPGETLELHHTVTMKSPTTAVRWVAVHYPKTSGGVATMVDLHANDP
                     AARDLTQTLPSGTPLSQPYWLREEGTAGMFRVDDPALIGRPENPPAFPVEFVFDIGGQ
                     TLIIPDEPVAISSDAKRGELRRRLDVIPPVSLSFASQVRLFTPGASRSVDVEAIAYRA
                     "
BASE COUNT          263 a          494 c          510 g          259 t
ORIGIN      
        1 gccatcgggc gtggcgatcc ttcaggacct gaagcgtttt gccaacctcg ggagcgtctt
       61 gatggtggcc gcccatccgg acgacgagaa cacgcagctg atcacgtacc tggcgcgcgg
      121 ccgcgggtat cgcacggcgt acctctcggt cacccgcggc gacggtgggc agaacgtgct
      181 gggcccggag ttcggtgagg agctcggcgt catccgcacc caggagctgt tgtcggcccg
      241 ccgactggac ggtggccggc agttcttcac ccgcgcgatc gacttcggat tttcaaaaga
      301 ttttcaggag acgctgggca tctgggatcg aaagcaagtg ctcggcgatg tcgtgcgcgt
      361 catccgcatc tttcggccgg atgtgatcgt cacgcgcttc tcacccaagc ccggtaacac
      421 gcacgggcat cacacggcat cggcggtgct ggctctggat gcattcaaaa ttgccggcga
      481 tccaaaggcg tatcccgagc aactcgtcgg cgacatgaag ccgtggcagc ccaagcgcat
      541 cttcatgaac ggcggcggac cgcgcgggaa tgacgcagcg ggcgcgaatc cttcggcgat
      601 tcgcatcgac gacggcggga ccgacccggt gctgggcgag tcgttcggcg gcatcgcccg
      661 ccgaagccgg gcaatgcaca agacgcaggg gttcggtaat ttcgcgattg gagccggcgg
      721 cggtggcccg caccaggagg cgttcgtcct gctcgatggc gagccggcga ccgcggatat
      781 tctcgacggc gtcgatacga cgtggaaccg cgtgcccagc ggtggcgccg ccatcgcaac
      841 cgccgtcgat gcggcgatca aatcgttcag cgtcgacaac cccgcggcgt cggcgccggc
      901 gctgttggac atccgcggca agcttgccgg cggggcggcc gatgacattg tcgtcaacga
      961 gaagcgccgg cagctcgatc gtatcatcgc cgactgcatc ggcctttctg tgcagacgac
     1021 cgtgccccgc gccgaatacg tgccggggga aacgctcgaa ttgcatcaca ccgtcacgat
     1081 gaaatcgcca acgacggcgg tgcgctgggt ggcggtgcac tatcccaaaa catcaggcgg
     1141 tgtggcgacc atggttgatc tgcacgcgaa cgacccggcc gcgcgcgacc tgacgcagac
     1201 gttgccgtcc ggcaccccgt tgagccagcc gtattggctg cgcgaagagg gaaccgcggg
     1261 gatgttccgc gtggatgatc cagccctgat cggtcgcccg gaaaatccac cggcgtttcc
     1321 ggtggagttc gtcttcgaca tcggcggcca gacgctcatc attccggatg aaccggtcgc
     1381 catcagcagc gacgccaagc gcggcgagct tcgccgccgg ctcgacgtca tccccccggt
     1441 gtcgctgagt ttcgcctcgc aggtgcggct gtttaccccg ggcgcttcac ggtcggtcga
     1501 tgtcgaagcg atagcctatc gcgcca
//
LOCUS       DATIMH010000626         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_440673, whole genome shotgun sequence.
ACCESSION   DATIMH010000626 DATIMH010000000
VERSION     DATIMH010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440673"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VLI90_10800"
     CDS             <1..698
                     /locus_tag="VLI90_10800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSV14740.1"
                     /translation="SEKIPLTKMRAAIATALQRSKQNVPHFYVSTDVDVEELTTLRAR
                     LNKKLEPQGLKISVNDFVSKATAAALRRHPALNAHFTGDAITRFGDVHLGIAVAIPDG
                     LIVPVIKSADQMGLVELQQRTAELAKKAKAQKLKGDEMKGATFTISNLGMYGVKEFSA
                     IINPPESAILAIGGTAWQPVVKDVNGSKEIVPGQVMNLTLSCDHRVVDGATGAKFLQE
                     LKAILESPLTMLV"
     gene            1053..2561
                     /locus_tag="VLI90_10805"
     CDS             1053..2561
                     /locus_tag="VLI90_10805"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSV14741.1"
                     /translation="MKTETPAPVEASFASFGLSEPILAGVTAAGYKAPTPIQAAAIPV
                     VLSGKDLIAQARTGTGKTAAFGLPAMSRLKFDGSVEILVLTPTRELAGQVSDELFRLG
                     RIAKVRSVAVYGGQSSSRQIELIRRGCQIVVATPGRLLDHLESKRMGKFAPSTIILDE
                     ADEMLDMGFMDDLKAIFKHLPAQTAEGEAEGGEGKRRQTLMFSATMPPPIRKLAQEIL
                     KQPVTINLTQQEATNTDVEQSFYVIEEGERLDAVVRIIDAEEPAKSILFCRTKRETDE
                     LCEVLIARGCQARALHGDIEQGQRQEIIRAFKDGRLEMLVATDVAARGLDVTGVTHVI
                     NFHMPFDQAGYVHRIGRTARAGQKGKAITLVNPFEFRALKRLQSAIKASFIHREVPSA
                     ADIHAKEDAKLIRKVCLQEVSDEAVEMLSNLEEQLDPTQIACKLITMILAKRKVRGPE
                     AIGVRGPRLKTLLSGGGAGAGGGGGGYRGGGGGHGDRRDRFRGKGFKDRRPR"
BASE COUNT          538 a          776 c          819 g          437 t
ORIGIN      
        1 aatccgagaa gatccccctg accaagatgc gcgccgcgat cgccaccgcg ctccagcggt
       61 cgaagcagaa cgtcccgcac ttctacgtct cgaccgacgt cgacgtcgaa gagctcacca
      121 ccctccgcgc ccgcctcaac aagaagctcg aaccccaggg cctcaagatc tccgtcaacg
      181 acttcgtctc caaggccacc gccgccgccc tccgccgcca ccccgccctc aacgcccact
      241 tcaccggcga cgccatcacc cgcttcggcg acgtccacct cggcatcgcc gtcgccatcc
      301 ccgacggcct catcgtcccc gtcatcaagt ccgccgacca gatgggcctc gtcgaactcc
      361 agcagcgcac ggcggagttg gccaagaaag ccaaagccca gaagctcaaa ggcgacgaga
      421 tgaaaggcgc caccttcacc atctccaacc tcggcatgta cggcgtgaaa gaattcagcg
      481 ccatcatcaa cccgcccgaa tccgccatcc tggccatcgg cggaaccgcg tggcagccgg
      541 tggtcaaaga cgtcaacggc agcaaggaaa tcgtgccggg gcaagtgatg aacctcacgc
      601 tctcgtgcga tcaccgcgtt gtggatgggg cgacgggagc caaattcctg caggaactta
      661 aagcgattct tgagtcgccg ctgacaatgc tcgtgtgagg agaatgcatg acaccgatac
      721 gggaacgaaa atataattgc tatgtcgacg ccgttcgaat tggcgtccta ttcggggtcg
      781 cgctagccat tgcgattcgc tattttatgg aaagttatcg ggacgcggat tcgtttccgc
      841 aaagcgcgat aaaggaaggg gcgggggggg ttgcgatttg cacggcgctg gcggcgtgcg
      901 tcataggatt gtgccagggg ttacgggcgg gaatgaagaa cgcaagctct gtagggatct
      961 attagctgca cgcgcggaga atttcgcgcc gacaaggatt tcaccaaaga tgcggcgcgg
     1021 acccgtgtcg ccataaccgg agttcaattg tcatgaaaac cgaaacaccg gcgcccgttg
     1081 aggcgtcttt tgcgtcattt ggattgagcg aacccatttt ggccggcgtc accgccgcgg
     1141 gctataaagc gcccacgccg atccaggccg cggccatccc cgtcgtcctt tccggcaaag
     1201 acctgatcgc gcaggcgcgc acaggcaccg gcaaaaccgc cgccttcggc ctgcccgcga
     1261 tgagccgcct caagtttgat ggcagcgtgg aaatcctcgt gctcacaccg acacgggagc
     1321 tggcgggtca ggtcagcgac gaacttttcc gcctcggccg catcgccaaa gtgcgctcgg
     1381 tcgctgtata cggcgggcag tccagcagcc ggcagattga actgattcgt cgcggttgtc
     1441 agattgtggt ggcgacgccg gggcggcttt tggatcatct cgaatcgaag cgcatgggaa
     1501 agtttgcccc gtcgacgatc attctcgacg aagccgacga aatgctcgac atgggcttca
     1561 tggacgactt gaaggcgatt ttcaagcatc tgcccgcgca gacggcggag ggcgaggcgg
     1621 aaggcggcga aggaaaacgc cggcagacgc tgatgttctc cgccaccatg ccgccgccga
     1681 tccgcaagct ggcgcaggaa atcctgaagc agccggtgac gatcaacctc acgcagcagg
     1741 aagcgacaaa cacagatgta gaacagtcgt tttatgtgat cgaagagggc gagcggctcg
     1801 acgcggtggt gcggatcatc gacgcagagg agccggcgaa atcgatcctg ttctgccgca
     1861 ccaagcgtga aaccgatgaa ctgtgcgaag tgctcatcgc ccgcggctgc caggcgcggg
     1921 ccctgcatgg cgacatcgag cagggccagc ggcaggagat catccgcgcg ttcaaagacg
     1981 gccggctgga aatgctcgtc gcgaccgatg tggcggcccg cgggttggat gtgacgggcg
     2041 tgacgcacgt gattaatttt cacatgccgt ttgatcaggc gggctacgtg caccgcattg
     2101 gccgcacggc acgggcgggg cagaagggca aggcgattac gctggtgaat ccgtttgagt
     2161 ttcgcgcgct caagcggctg cagtcggcga tcaaggcgtc gttcattcac cgcgaagttc
     2221 cttcggcggc ggatatccat gccaaggaag acgccaagct gatccgcaaa gtctgcctgc
     2281 aggaagtttc cgacgaggcg gtggagatgc tctcgaattt ggaagagcag ttggatccga
     2341 cgcagattgc ctgcaagctg atcacgatga ttctggcgaa acgaaaagtg cgcgggcccg
     2401 aagcgatcgg cgtccgtggg ccgcggctta agacactctt gagcggcggc ggtgcggggg
     2461 ccggcggggg tggcggcggg tatcgaggcg gcggcggcgg acatggagat cggcgggatc
     2521 gtttccgcgg caagggcttc aaagatcggc ggccgcgctg atgctggggc
//
LOCUS       DATIMH010000627         8454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2460129, whole genome shotgun sequence.
ACCESSION   DATIMH010000627 DATIMH010000000
VERSION     DATIMH010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460129"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3759)
                     /locus_tag="VLI90_10810"
     CDS             complement(<1..3759)
                     /locus_tag="VLI90_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HSV14742.1"
                     /translation="MSRRPIHFRMETLEQRVLFAAATVSFGNVQQTIDGFGASSAWSS
                     GTIPAAALDQIYSPISGAGLSLLRSRIQPSGGSSENQMMEQVGAYGVRAWSTPWTPPR
                     EYKTNHDNNNGGNLDPAHYQDYANQIANYVQDVTRQGIPLYAVSIQNEPNWTASYESA
                     RWDASSFAAFLPYLGQTFAARGITATKIMLPEELNWDFTFASTIMGDPNLSQYVGILA
                     AHNYGETATSWKPVSIANGKQVWETEVTSGLTAEWDTAIDEADDIHQAMTLGNASAYH
                     YWWLSSNGTTSGLTNNWTPTKRLWAMGQYSRFVRPGWVRVGETDDGGLDITTFKDPAS
                     GKFAIVTVNTSTVNPVTESFTLSGLTADNVTPYVTSTTESLAAHAAIALTGGGTIFSA
                     TIAPSSIETFYGVSNTAAAVQVPGNLMAVPQFGNMTTRVALSWTDNSSGETSYTVERS
                     TDGTNFTVVTSTLAANTTTYIDAGRSENTKYYYRVKPTNGDATTYSAVASTATILQSP
                     SSVSGTRNASGINLTWSIGSTASTGVTVDRSLDGVTWTNIATLNTRATSYADMIPEYS
                     SGQIYFYRVRNTAGGGSTSAQASWNTGLTTPLAFSAAQVTATTATFTWGSPQGASGAW
                     IEQFNSATGTWSVVSPSSLRATDGTWTLSGLTPTTSYSFRMRASAQADNLYSGYTGSI
                     AITTPFQPVWYKANENSGTTLEDSFTAAHNATLSGVSSFTSGVSGNALVISGGYATLP
                     AGIVSGLNDFTIATWVKVSSLSDGARIFDFGTATTNTMYLTAQAPGTAGLPQFVITTS
                     AGTQQITSSTPIALNNWTHIAVTLSGNSATMYINGVAAGTNTGMTIHPVALGSTTQNY
                     FGKSQGADPALLGSLDDFQIYGRAFSSGEVQVLATPSTQLAWYKADESLGGVLSDSSG
                     AHNDATLGGSFSFTAGVAGNAVALSGGVANLPTGIVGGLNNFTIAGWVKLASLDTWAR
                     VFDFGNSTSDYMFLTPKSSSGLPRFGINAGSGEQQINSNIALPLNTWAHVAVTLSGNV
                     STLFINGAAVGTNSSMTTHPAALGATTKNYLGDSQFSADPSLQGSLDDLRVYSRALTS
                     TEIQQLVYPTLATSAAAGSTTATTAALSVLGADQTEGESRLTYTWSVTGTPPAPVTFS
                     INGSNAAKNTTATFAKAGTYNFLVTITNAANFSVTSSISVTVNPTATTVNVSPTPVTV
                     AGGSSKQFSAAVLDQFGQTISGAAATWSTSGGGTVSSSGSFTASQSGGS"
     gene            complement(3762..4598)
                     /locus_tag="VLI90_10815"
     CDS             complement(3762..4598)
                     /locus_tag="VLI90_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HSV14743.1"
                     /translation="MKLTALLIALTAAVALADPATKPAAPPHRWSAAVPSERTDPNSK
                     LVHQQLVAKAKAGGIDLYFLGDSITRRWGCTDAIWAANLANWKHNFYGWNAGNFGWGG
                     DLIQNILWRIQNGELDGVNPKVIVILAGTNNIGNKPGGDRKVADIVAGFDALLKTCRE
                     KAPQAKIIITAIFPRNDNPAVVPEIERVNEQLAKRADGKEIFYVSINDKLADANGKLL
                     DGMTVDKLHLTPRAYQIWADALRPLLTELLGPPAQTDHAPPPTGDPSAAPSTAPSAHG
                     KT"
     gene            complement(4595..7510)
                     /locus_tag="VLI90_10820"
     CDS             complement(4595..7510)
                     /locus_tag="VLI90_10820"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HSV14744.1"
                     /translation="MTSGRALLPTLLILPLLLAAVAQVARADYVDAQESPGAFSIVQA
                     GHATSIYVDSQDFPGVIRAAGDLADDIHKVSGASAEVVRSAKDFTPRAVLIGTIGKSA
                     IIDRLVGDRKIDVSSSAGKWEACVTAVVANPMPGVESALVIAGSDKRGTIYGIYELSE
                     QMGVSPWYWWADVPPRHRNAVFVRAGTYVQGPPAVKYRGIFLNDEAPALSGWAKAKFG
                     GLNQKMYTRVFELLLRLKANYLWPAMWGNAFNEDDPANPRLADEYGIVMGTSHQEPMM
                     RAQAEWDRRHKGADWNFATDPAGMENFWRDGIKRNKNYENLITIGMRGRNDTPMIENA
                     TVEQSMALLEKIVGVQRRIIAEEVNPDVTKVPQLWCLYKEVQSYYENGMRVPDDVTLL
                     WSDDNWGNLRRVPTADERRRAGGAGIYYHFDYVGDPRDYKWLNTNPLPKIWEQMHTAY
                     RYGADRVWIVNVGDLKPMELPIDFFMRLAWDPDKIGPDKIGEFTRHWAAEQFGPEHAE
                     EIADILSKYAKYNGWRKPELLEPTTFSLINNREAERVEQAWQDITARAEQIYATLPPE
                     TRDAFYQLVLYPTKASATVAELYIAAGRNQLYAKQRRASADDQAARVRELFKQDQELS
                     NTYNHTMAGGKWNHMMDQTRIGYTSWQDPKTNVMPAVQELAVSASAALGVTVEGSASS
                     WPGDAGEAQLPVFDSVNHQRYAIEVFNRGGDAFDFTCTADQPWVTLSKTTGRVERDER
                     IWVNINWPMAPVGDGNAVITIARTGGERVAVSLKTIRSTDVKRETLDAFGGLTGPIAI
                     AAEAATSNVPAADARWVKIPDYGRGASGMCVLPATAASVTPPSDAAPRLEYRVYLPQA
                     GQVQVDAVIGPSLDFVAGRGLRYAVSIDDEPPQVVDVFAKQFRTHREWQQAVKDNARK
                     RSSTHAIAAAGVHIVKIWMVDPGVVLEKLVVHQGDVPPSYFGPPEALAPDSQGVMQQ"
     gene            complement(7533..>8454)
                     /locus_tag="VLI90_10825"
     CDS             complement(7533..>8454)
                     /locus_tag="VLI90_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14745.1"
                     /translation="EQLCNWVLMATCYVAKSGDDIWLRRNAGTIRACLNSLIARGGES
                     GLVRYDSARCEGGQEITTYDSLDASLAQTRNNVYMAVKCWASYVGLALLVEALGDSSA
                     ADMAGERAASVAATVVANAGADGVIPAIFERNNPGHASRILPAAEGLVYPLYWEQAGF
                     APIRLAKLLESPATTKLIDALRRHVTALLVDPQRRNIFADGGIRLSSTSGNSWMSKIA
                     LFQHVARGWLGAADDPAIARILADADAAHVKWQTDGSAFWACSDQIVHGQAKGSRYYP
                     RIVTAGLWLDEPRRRKSAEAARPVSRVIAK"
BASE COUNT         1366 a         2765 c         2665 g         1658 t
ORIGIN      
        1 cgacccgccg ctttgggaag cggtaaacga tccgctggag ctgactgtcc cgccaccgct
       61 ggttgaccac gtcgccgccg cgccgctgat cgtctgtccg aactgatcga gcacagctgc
      121 gctgaattgc ttgctgctgc cgccagccac cgtcaccggt gtcggcgaca cgtttacggt
      181 cgttgccgtc ggattgaccg tgacgctgat ggagctggtc acggaaaagt tcgcggcatt
      241 ggtgatggtc acgaggaagt tgtacgtgcc ggccttggcg aacgtggcgg tggtgttctt
      301 cgccgcgttg ctgccgttga tggagaacgt caccggcgcc ggcggtgtgc cggtgacgga
      361 ccaggtgtaa gtcaatcgcg attccccctc cgtttgatcg gcccccagga ccgacagcgc
      421 ggcggtggtg gccgtcgtcg atccggccgc ggcgctggtg gcgagcgtcg gataaaccag
      481 ttgctggatt tccgtgctcg tcagcgcgcg actgtagacg cggagatcgt cgagactgcc
      541 ctgcagcgat gggtcagcgc tgaactgcga atcgccgagg taattcttcg tcgtcgcgcc
      601 caacgcggcg gggtgggtcg tcatgctcga gttcgtgccg acggccgcgc cgttgataaa
      661 aagcgtgctg acgtttcccg acagagtgac cgcaacgtgc gcccacgtgt tcagcggcag
      721 cgcgatgttg ctgttgatct gctgctcgcc ggacccggcg ttgatcccga agcgcggcaa
      781 tcccgaactc gacttgggcg tgaggaacat gtagtcgctc gtgctgttgc cgaagtcgaa
      841 cacgcgcgcc caggtgtcca gcgacgcgag cttcacccag ccggcgatcg tgaaattatt
      901 gagccccccc acgatgccgg tcggtaggtt agcgacacca ccggacagcg cgacggcgtt
      961 gcctgccacg cctgcggtga agctgaacga gccaccgagg gtggcatcgt tgtgcgcgcc
     1021 ggaggaatcg gacaacacac cgcccagcga ttcatccgct ttgtaccaag ccaactgcgt
     1081 gctcggcgtg gccagcactt ggacttcgcc gctggaaaac gcccggccgt aaatctgaaa
     1141 atcatcgagg ctgccgagca gggccggatc ggctccctgc gatttgccga aataattctg
     1201 cgtcgttgac cccagcgcca ccggatggat cgtcatcccg gtattcgtgc cagcggccac
     1261 accgttgatg tacatcgtcg cgctgttgcc cgagagcgtc accgcgatgt gcgtccagtt
     1321 gttcagcgcg atcggcgtgc tgctggtgat ctgctgagtg cccgcgctgg tcgtgatgac
     1381 gaactgcggc agccccgcgg tgcccggcgc ttgagccgtc aggtacatgg tgttcgtcgt
     1441 tgccgtgccg aagtcgaaga tccgcgcgcc atcgctgagc gacgacacct tgacccacgt
     1501 tgcgatcgtg aagtcgttca acccgctgac gatccccgcg ggcaacgtcg catacccacc
     1561 gctgatcacc agcgcgttac cgctcacacc ggaggtgaag ctgctgacac ccgagagggt
     1621 agcgttgtgc gcggcggtga acgaatcctc caacgtcgtg ccgctgtttt cgttggcctt
     1681 gtaccagacc ggctggaacg gcgtcgtgat cgcaatgctg ccggtgtagc cggagtagag
     1741 attgtccgcc tgagcgctgg cccgcatgcg gaagctgtaa gaggtcgtgg gagtcagtcc
     1801 gctcagcgtc cacgtgccat cggtcgcccg caggctgctg ggcgagacga cgctccatgt
     1861 accggtggcg gagttgaact gctcgatcca tgcccccgat gcgccctgcg ggctgcccca
     1921 ggtgaacgtc gctgttgtcg cggtcacttg agcggccgag aacgccaaag gcgtcgtcaa
     1981 cccggtgttc cacgacgcct gcgctgatgt gctcccgccg ccggccgtgt tgcggacgcg
     2041 gtaaaagtag atctgcccgc tggaatattc ggggatcatg tcggcatacg acgtggcccg
     2101 cgtgttgagc gtggcgatgt tcgtccaggt gacgccgtcg agtgagcggt cgaccgtcac
     2161 accggtggag gcggtcgacc cgatgctcca cgtgaggttg atcccggagg cgttgcgcgt
     2221 tccgctgacg cttgaaggag attgaagaat cgtggcggtc gatgcgaccg cgctgtacgt
     2281 ggtggcgtca ccgttggtgg gcttgacgcg atagtaatat tttgtgttct ccgagcgacc
     2341 tgcgtcgatg tacgtcgtgg tgttggccgc gagggtactc gtgacaaccg taaagttagt
     2401 gccgtcggtt gagcgctcga ccgtgtagct cgtttcgccg ctggaattgt cggtccagga
     2461 caacgcgacc cgcgtggtca tattgccaaa ctgcggcacc gccatgagat tgcccggcac
     2521 ctgcacggca gctgcggtgt tgctcacgcc ataaaacgtc tcgatactgc tgggggcgat
     2581 ggtggcgctg aagatcgtgc cgccgccggt caaggcgatg gcggcgtggg ccgccaggct
     2641 ttcggtggtt gatgtcacgt acggcgtcac gttgtcggcg gtgaggccgc tgagcgtgaa
     2701 gctctccgtc actgggttca cggtgctggt gttgacggtg acgatcgcga actttcccga
     2761 agctgggtcc ttgaacgtcg tgatatccaa cccgccgtcg tcggtttcgc cgacgcgcac
     2821 ccaacctggg cggacaaacc ggctgtactg ccccatcgcc cacagtcgct tcgtcggcgt
     2881 ccagttgttc gtcagtccac tcgtggtgcc gttggaactg agccaccaat agtgataggc
     2941 gctagcgttg ccgagcgtca tcgcctggtg gatgtcgtcg gcctcgtcga tcgcggtgtc
     3001 ccactcggcc gtcaggcccg aagtgacttc cgtttcccaa acctgcttgc cgttggcgat
     3061 gctcacgggc ttccacgatg tcgcggtctc accgtagttg tgcgccgcga ggatgccgac
     3121 gtactgcgac aagtttggat cgcccatgat cgtgctggcg aacgtgaaat cccaattcag
     3181 ttcttccggc agcatgatct tcgtcgcggt gatcccgcga gccgcgaacg tctgcccaag
     3241 gtacggcagg aacgccgcga atgatgaggc atcccaccgc gctgattcgt agctggccgt
     3301 ccaattcggt tcgttctgga tcgacaccgc atacagcggg atgccctgcc gcgtcacgtc
     3361 ctgcacgtaa ttcgcgatct ggttggcgta atcctggtaa tgcgccggat cgagattgcc
     3421 gccgttgttg ttgtcgtgat tggtcttgta ttcacgcggc ggtgtccacg gggtggacca
     3481 tgcgcgcacg ccgtacgcgc cgacctgctc catcatctgg ttttcgctcg aaccacccga
     3541 aggctggatg cgactgcgca gcaggctaag cccggcgccg gagatgggcg agtaaatctg
     3601 atcgagcgcc gcagcgggga tcgttcccga cgaccaggcc gaagaagcgc cgaagccgtc
     3661 aatggtctgc tgcacgttgc cgaagctcac cgtggcggca gcgaagagca cgcgctgctc
     3721 gagcgtttcc atgcgaaaat ggatcgggcg gcgcgacatg ctcaggtctt cccatgcgca
     3781 gaaggcgcgg tggatggcgc cgcggaagga tcgccggtcg gcggtggcgc gtgatctgtt
     3841 tgcgccggtg ggccaagcag ctccgtcagc agcggccgca gtgcatcggc ccagatctgg
     3901 tacgcccgcg gcgtcaggtg cagcttgtct accgtcatgc cgtccaagag cttgccgttg
     3961 gcgtcggcaa gtttgtcgtt gatgctgacg tagaagatct ccttgccgtc ggcgcgcttg
     4021 gccagttgct cgttcacgcg ctcgatctcc ggcaccacgg cggggttgtc gttccttggg
     4081 aagatggcgg tgatgatgat cttcgcctgc ggcgcctttt cgcggcaggt cttcagcagc
     4141 gcgtcgaagc ccgcgacgat gtcggcgacc ttcctgtcgc cgccgggctt gttgccgatg
     4201 ttgttcgtcc cggccaggat cacgatcacc ttcgggttca cgccatccag ctcgccgttc
     4261 tggatgcgcc acagaatgtt ctgaatgagg tctccgcccc aaccgaagtt gccggcgttc
     4321 cagccataaa agttgtgctt ccagttggcg aggtttgccg cccagatcgc atcggtgcag
     4381 ccccagcggc gggtgatcga atcgccgagg aaatacagat cgatcccgcc agctttggct
     4441 ttggcgacga gctgctggtg cacgagcttc gaattgggat cggtgcgttc cgagggaacc
     4501 gccgccgacc aacgatgcgg cggggccgcc ggcttggttg ccggatcggc aagcgcgacc
     4561 gccgcggtca acgcaatcag aagagccgtc aatttcattg ctgcatcact ccctggctgt
     4621 cgggcgccag cgcctccggt ggaccaaaat agctcggcgg tacgtcgcct tggtgcacga
     4681 cgagcttctc gagcaccacg cccggatcga ccatccagat cttcacgatg tggacgcccg
     4741 ccgcggcaat cgcgtgcgtt gagctgcgct ttcgcgcgtt atccttcacc gcctgctgcc
     4801 actcccggtg cgtgcggaat tgcttggcga acacatccac gacctgcggc ggctcatcgt
     4861 cgatcgacac cgcataacgc aacccccggc ccgccacgaa atccaagctc gggccgatca
     4921 ccgcgtccac ctgcacttgc cccgcctgcg gcagatagac ccgatactcc agccgcggcg
     4981 ccgcgtcact cggcggcgtc acgctcgccg ccgtcgccgg cagcacgcac atgcctgacg
     5041 ccccgcggcc gtagtcgggg atcttcaccc aacgcgcatc cgccgccggg acattgctgg
     5101 tcgccgcttc agccgcgatc gcgatcggtc cggtcaatcc accgaaggcg tcgagcgtct
     5161 cgcgcttcac gtcggtggac cggatcgtct tcaacgacac cgccactcgt tcgccgccgg
     5221 tgcgcgcaat ggtgatcacc gcgttgccat cgcccacggg cgccatgggc cagttgatgt
     5281 tcacccaaat ccgttcgtcg cgctccaccc ggccggtcgt cttgctcagc gtcacccacg
     5341 gctggtccgc cgtgcacgtg aaatcaaatg cgtcgccgcc gcggttaaag acttcgatgg
     5401 cgtagcgttg atgattgacc gaatcgaaga ccggcagctg cgcttcgccc gcatcacccg
     5461 gccaactcga tgctgagcct tccaccgtga cgcccagcgc cgcgcttgcc gacaccgcca
     5521 gttcctgcac ggccggcatc acgttggtct tggggtcctg ccagctggtg taaccgatgc
     5581 gggtctggtc catcatgtgg ttccatttcc cgccggccat cgtgtggttg taggtgttgc
     5641 tcagctcctg atcctgcttg aacaactcgc gcacgcgcgc ggcttggtcg tcggcactgg
     5701 cgcgccgctg cttcgcgtaa agctgattgc gcccggcggc gatgtacaac tccgccaccg
     5761 tcgccgatgc cttggttgga tacaggacca gttgatagaa cgcgtcgcgg gtctccggcg
     5821 gaagcgtcgc gtagatctgc tccgcgcgcg cggtgatgtc ctgccacgcc tgttcgacgc
     5881 gctctgcctc gcgattgttg atcaagctga acgtcgtcgg ctccagcagc tccggcttgc
     5941 gccagccgtt gtacttcgcg tacttgctga ggatgtcggc gatctcctcg gcgtgctcgg
     6001 gcccaaactg ctccgccgcc caatgccgcg taaactcgcc gatcttgtcc gggccgatct
     6061 tgtccggatc ccacgccagc cgcatgaaga agtcgatcgg cagctccatt ggtttcaaat
     6121 cgccgacgtt cacgatccac acgcggtccg cgccgtagcg atacgcggtg tgcatctgct
     6181 cccagatttt gggcagcggg ttggtgttga gccatttgta atcgcgcggg tcgccgacgt
     6241 aatcgaagtg atagtaaatg ccggcgccac ccgcgcggcg gcgctcgtcg gcggtcggca
     6301 cccggcggag gttgccccag ttgtcgtccg accacagcag cgtcacgtcg tccggcacgc
     6361 gcatgccgtt ctcgtaatac gactgcactt ccttgtacag gcaccacagc tgcggcacct
     6421 tggtcacgtc gggattcact tcctcggcga tgatccgccg ctgcacaccg acgattttct
     6481 cgagcagcgc catgctctgc tcgaccgtcg cgttctcgat catcggcgtg tcgttgcggc
     6541 cgcgcatgcc gatcgtgatc aggttctcgt aattcttgtt gcgtttaata ccgtcgcgcc
     6601 agaagttctc catccccgcg gggtcggtgg cgaagttcca atccgcgccc ttatgccggc
     6661 ggtcccattc cgcctgggca cgcatcatcg gctcctggtg actggtgccc atgacgatgc
     6721 cgtattcgtc cgccagccgc gggttggccg gatcatcctc attgaacgcg ttgccccaca
     6781 tcgcgggcca gaggtagttc gccttcagcc gcaggagcag ctcgaagacg cgggtgtaca
     6841 tcttctgatt cagcccaccg aatttcgcct tggcccaacc gctgagcgcc ggcgcttcgt
     6901 cgttcagaaa gatgcctcga tacttcaccg ctggcggacc ttgcacatat gtgccggcgc
     6961 gcacaaagac ggcgttgcga tgccgcggcg gcacgtccgc ccaccagtac cacggcgaca
     7021 cgcccatctg ctcggacaac tcgtagatgc cgtagatcgt cccgcgcttg tcgctgccgg
     7081 cgatgaccag cgcgctctcc acgcccggca tcggattcgc gaccaccgcg gtaacgcacg
     7141 cttcccactt gccggcgctc gacgacacat caatcttccg atcgcccacc agccggtcaa
     7201 tgattgcgct cttgccgatc gtgccgatga ggacggcgcg aggcgtgaaa tccttggccg
     7261 agcgcacgac ctccgctgac gcgccggata ctttgtgaat gtcatcggcg agatcaccgg
     7321 cggcgcggat gacgccgggg aagtcctggc tgtcgacata aatagaggtg gcgtgacccg
     7381 cctgcacgat cgagaacgcg cccggcgatt cctgagcatc gacgtaatcc gcccgtgcga
     7441 cctgcgcgac cgctgccagc agcagcggca ggatcaacag tgtcggcaat agcgctcgac
     7501 cgctcgtcat ctgctcaact ccagcatgtc gattattttg cgataacgcg actcaccggg
     7561 cgggccgctt ccgcggactt gcgccgccgc ggttcgtcca gccacagccc cgccgtcacg
     7621 atgcgcgggt aataccggct gcccttggct tgcccgtgca cgatctggtc cgagcacgcc
     7681 cagaacgcgc tgccgtcggt ctgccacttc acatgggccg cgtcggcatc ggcgaggatg
     7741 cgcgcgatcg ccgggtcgtc ggccgcgccg agccacccgc gcgcgacatg ctggaacaag
     7801 gcgatcttgc tcatccacga gttgccggat gtggacgaga gccggatgcc gccgtcggca
     7861 aaaatattcc gccgctgcgg atcgaccagc agcgcggtca cgtgacgccg cagcgcgtcg
     7921 atgagcttcg tcgtcgccgg cgactccagc agtttcgcca accgtatcgg ggcgaaaccc
     7981 gcctgttccc aatacagcgg atagaccagc ccctccgccg ccggcaggat gcgcgatgca
     8041 tggccagggt tgttgcgctc gaagattgcc gggatgacgc cgtcagcgcc ggcgttggcg
     8101 acgaccgtcg cggccacgga ggcggcgcgc tccccggcca tgtcggccgc cgatgaatcg
     8161 ccgagcgctt cgaccagcag cgcgagcccc acatagctcg cccagcactt caccgccatg
     8221 tagacgttgt tgcgcgtctg cgccagcgac gcgtcaagcg aatcgtacgt cgtgatctcc
     8281 tgtccgcctt cgcagcgggc cgagtcgtat cgcaccaatc ccgattcacc gccgcgggcg
     8341 atcaagctgt tcaggcacgc gcggatcgtc ccggcatttc ggcgcagcca aatgtcgtcg
     8401 ccgctcttgg caacgtagca cgttgccatc agtacccagt tgcacagttg ctcg
//
LOCUS       DATIMH010000628         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5064340, whole genome shotgun sequence.
ACCESSION   DATIMH010000628 DATIMH010000000
VERSION     DATIMH010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5064340"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1813
                     /locus_tag="VLI90_10830"
     CDS             122..1813
                     /locus_tag="VLI90_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HSV14746.1"
                     /translation="MPQPLGRLCLVLHGHLPYVLHHGVWPHGEAWLFEAAAETYLPLL
                     ELLDELAEHNLQSVLTIGLTPVLLEQLASPRFKAAFPAYLKGRQELAERDAREFASAG
                     DDHLSDRARDWQAWYADRLAHFQRIKRDIPRALSRHINGGLIEVLTSAATHAYLPLLL
                     HDESIRAQLAAGVAATERILGVRPPGFWLPECAYRPSYEQWQPAVLNLPPRGRPGIET
                     FVENAGLSHFFVDTHLVAQAQPIALMKSDGVHATSDALVFWDLKRGWGNVLDPVGVVS
                     DPRVPRCFAFARHPRVSEQVWSGVIGYPGAAEYLEFHLKHGEQGLRYHRVTDHHTPMH
                     QKAPYDPALVPGKVYEHAHHFCTVVRETLSDYTAQTGRIGTIVAPFDAELFGHWWFEG
                     PRFLRDVLLTLAHDGAVELSTPQRALEQSPPDKVVRMPEGSWGEGGHHHVWLNEKTRW
                     LWEIEYRAESRFTDLLRDLPWQQRPAVRKMLECTARELLLLQSSDWPFVIHSGGATDY
                     GVSRFAGHATRFDRLATLATLLAAGKKLDPVQRTEILEAAAHDTVFENIDLNWWE"
     gene            complement(2122..2475)
                     /locus_tag="VLI90_10835"
     CDS             complement(2122..2475)
                     /locus_tag="VLI90_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14747.1"
                     /translation="MVIAYHLIWTRYGWWLPNDLRGSMSRGIASDIISDLGKLHYGRK
                     RIQPASKDIRGFYADAAAALEHPLLSSARLLWRSSALRLNKRFTPAVTPATRAPLCRT
                     TFTCSFESTGIWPRR"
     gene            complement(2552..>2759)
                     /locus_tag="VLI90_10840"
     CDS             complement(2552..>2759)
                     /locus_tag="VLI90_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14748.1"
                     /translation="DTETPIDLIHAIRPDVLVKGADYQKEQVVGWDFVENCGGSVALA
                     PLIEGRSTSAVIQRVLEAHGSKST"
BASE COUNT          497 a          916 c          843 g          503 t
ORIGIN      
        1 agatttatat tcccgccaga tcagtgcagg tgctggcgcc gcagtaaaac gccgcttgcg
       61 gctgagcatc tgttcgcttt gtgaacgaat gatgctaagc cgcaagcggc caccctccca
      121 catgccccaa cccctcggcc gcctgtgtct ggtccttcac ggtcacctgc cgtacgtcct
      181 gcaccacggc gtctggccgc acggggaagc gtggctgttt gaagccgccg ccgaaaccta
      241 tctgccgctg ctggaactgc tcgatgaact ggcggagcac aacctgcagt cggtgctgac
      301 gatcggctta acgccggtgc tgctggaaca gctcgcgagc ccgcgtttca aggctgcgtt
      361 tcccgcgtat ttgaaagggc ggcaggaact ggccgagcgc gacgcccgtg agttcgcatc
      421 ggcgggcgac gatcatctct ccgatcgcgc ccgcgactgg caggcatggt acgccgatcg
      481 cctcgcgcac tttcagcgga tcaagcgcga catcccgcgg gcgctgtcgc gccacatcaa
      541 cggcggtttg attgaagtgc tgaccagcgc ggcaacgcac gcgtatttgc cgctgctgct
      601 gcacgatgag tcgatccgtg cgcagctcgc cgcgggcgtg gcggcgacgg agcgaatcct
      661 cggcgttcgg ccgcccgggt tctggctgcc cgaatgcgcg tatcggccat cgtacgaaca
      721 gtggcagccc gcggtgctga acctcccgcc gcgggggcgg ccggggatcg aaacgttcgt
      781 ggaaaacgcc ggactgtcgc actttttcgt agacacgcac ctggtcgcgc aggcgcagcc
      841 gatcgcgctg atgaaatccg acggcgtaca cgcgaccagc gatgcgctgg tcttctggga
      901 cctcaagcgc ggctggggca acgtgctcga tcccgtcggt gtcgtcagcg atccgcgcgt
      961 gcccaggtgc ttcgcgttcg cgcgccaccc acgcgtcagc gagcaggtgt ggtccggcgt
     1021 aatcggttac cccggcgcgg ccgagtacct ggagttccac ctcaagcacg gcgagcaggg
     1081 gctgcgctac caccgcgtga ccgatcatca cacgccgatg catcagaagg cgccgtacga
     1141 tccggccctg gtgccgggca aggtgtacga acacgcccat catttctgca ccgtcgtgcg
     1201 cgagacgctc agcgactaca ccgcccagac cgggcgcatc ggcacgatcg tcgcgccgtt
     1261 cgacgcggag ctgttcggcc actggtggtt cgaaggcccg cgcttcctgc gcgacgtgct
     1321 gctgacgctg gcccacgatg gcgcggtgga gctatcaaca ccgcagcgcg cgctggagca
     1381 atccccgccg gacaaggtcg tccgcatgcc cgagggcagc tggggcgaag gcgggcatca
     1441 tcacgtctgg ttgaacgaaa aaacgcgctg gctttgggaa attgaatacc gcgccgagtc
     1501 acgcttcacc gatctgctgc gcgatctccc ctggcagcaa aggcccgcgg tgcgaaagat
     1561 gctcgaatgc accgcccgcg aactgctgct gctgcaaagc agcgactggc ccttcgtcat
     1621 ccacagcggc ggggcgaccg actacggcgt ctccagattc gccggccacg cgacgagatt
     1681 cgatcgcctc gccacgctgg ccacactact cgccgcgggc aagaagctcg atccggtgca
     1741 gaggaccgag attctggagg ccgcggcgca cgacacagtt ttcgaaaata tcgacctgaa
     1801 ctggtgggag tagacacacg gccgatcgcc gcatcgcccc cagcgtcgct tgcggtttcg
     1861 cagaccagaa tgcgctccac ttctctaccg aacgacccgc acccgcggcg gttcccaccc
     1921 gtcataccgc gtcacaaacg gccactcctg gcgcggttga tagatcttca acggttatct
     1981 tcgatgtacg caatggtgcg ttcgacgtct tggcgcgact ccaggaacac cttccaccct
     2041 ggacccccca gaccggaggg tctaactcgc gcatccccgc ttccttcaac gcaatctgcg
     2101 actctcgttg gaagctagcg atcatctcct cggccagatg ccggtgcttt cgaatgagca
     2161 ggtgaatgtg gtccggcata atggcgcacg cgtagcaggt gtaaccgcag gcgtgaatcg
     2221 tttgttcaaa cgaagcgccg acgaccgcca cagcagccgc gccgacgaaa gcaatggatg
     2281 ctcaagcgcg gccgcggcat cggcgtagaa gccgcgaata tcctttgacg ccggctggat
     2341 gcgttttctt ccgtaatgaa gttttccgag atctgagatg atatcgctcg cgatcccacg
     2401 cgacatgctg ccgcgcaaat cgttggggag ccaccagccg tagcgcgtcc aaatgaggtg
     2461 gtacgcgatg accatggctt tcccttcgtt tcagaacccg cgccttttgg tctgcgaaac
     2521 cgcaagcgac gctgttcgcc gtgcggcgat ctcacgtcga tttactcccg tgcgcttcca
     2581 ggacgcgctg gatcactgcg gatgtgcttc tgccttcgat caacggcgcc agcgcgacgc
     2641 tgccgccgca gttttctacg aagtcccagc cgacgacttg ctccttctga taatcagcgc
     2701 ctttcaccag cacatccggg cggatcgcgt gaatcagatc gatcggcgtt tcggtgtcg
//
LOCUS       DATIMH010000629         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2749346, whole genome shotgun sequence.
ACCESSION   DATIMH010000629 DATIMH010000000
VERSION     DATIMH010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2749346"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..540)
                     /locus_tag="VLI90_10845"
     CDS             complement(172..540)
                     /locus_tag="VLI90_10845"
                     /inference="COORDINATES: protein motif:HMM:NF042735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSV14749.1"
                     /translation="MSVKTVGQQFVQLCRQGKNFDVMRTMYAPNIISVEGDGKETAGQ
                     QPVIKKSEDWVSDKIFHGETVAGPFFNGANPDQFAVFFTLDVTPKASGKRITLEEVGV
                     YTVNPDDKITREQFFYDGAH"
     gene            complement(755..1420)
                     /locus_tag="VLI90_10850"
     CDS             complement(755..1420)
                     /locus_tag="VLI90_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSV14750.1"
                     /translation="MLMQESSTRKIYDIHSVFYDATFGRLVKRRIERAIEHMNISDTD
                     VVLDLGIGTGVSLNYYPNRGRIFGIDLSAGMLREARKKIRERGLDHATVFQADAMKLP
                     FADNSFDHVFISHVISVVSDPFKLVQEAQRVAKEGARIVIVNHFQSTNRFIAMVEKWL
                     CPLCTKLGWRSDLALQDLIRQTGVCIDYRYKLDNIDLWETVVITNNKSAIHHEIRGAT
                     LAA"
     gene            complement(1527..2258)
                     /gene="pgl"
                     /locus_tag="VLI90_10855"
     CDS             complement(1527..2258)
                     /gene="pgl"
                     /locus_tag="VLI90_10855"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSV14751.1"
                     /translation="MPPRQIKVFPTPQELTDDAARRIIAAAEEKLADLQHFFALVLSG
                     GSTPKALYETLAAEPYRSQINWSKVEIYFGDERCVPPDHPESNYRMAHEAMLSKLPIP
                     EPNIHRVHGELPPELAATEYGKLLKDKFGDGGADMVLLGMGDDGHTASLFPGTAALSE
                     AKHRCVANHVPKLNAWRITTTYPFLNKANQVLILVAGAAKAQRLTEVLEGQTDIQRLP
                     IQGIEPAGQLTWLLDAAAAGMHGEE"
     gene            2392..3246
                     /locus_tag="VLI90_10860"
     CDS             2392..3246
                     /locus_tag="VLI90_10860"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HSV14752.1"
                     /translation="MEYRVELDVFNGPLDLLLYLIKRDELDIYDIPIARITESYMHYV
                     AMLKDLRSDAGFDINVAGDFLVMAATLMEIKSATLLPRAPVVNETGEPTAAQELADPR
                     YELVKKLLEYKAFKDAAGALERQQYSHEARFPRFPAKLDGEDADEPPPVDLEEVQVWD
                     LLSAFDRLMKEVGVRKHKYHEVTYDDTPIDLHAADIEDRLKREGRLSLRGLVVGRKSR
                     SEMIGVFLALLELIREKKILVHQDAPLDDVLIEPAPEEHRRTYVGASLHLSDEAEKTP
                     TDDDAAVT"
BASE COUNT          615 a          951 c         1046 g          663 t
ORIGIN      
        1 ccacatgtgg atgaggtccg cgccgccggc cttttccgcc ggaccggaag cgctgcgcat
       61 gagatatcgc tgcagcggat atggcaggac ttccacgccg ggcgcgttca tcgtttccat
      121 aagctggttg cgaatcgcgc gggccaggcg gccggtgaac gcgctcgtcg atcagtgcgc
      181 gccgtcataa aagaactgct cgcgcgtgat cttgtcgtcc ggattgaccg tgtacacgcc
      241 gacttcttca agcgtgatgc gcttgccgct cgccttgggc gtgacgtcga gcgtgaagaa
      301 gacggcgaat tggtcggggt tggcgccgtt gaagaacggc ccggcgacgg tttcgccgtg
      361 gaaaattttg tcggagaccc agtcttcgga tttcttaatg accggctgtt gcccggcggt
      421 ctccttgccg tcgccttcga cggagatgat gttgggcgcg tacatcgtgc gcatcacatc
      481 gaagttcttg ccctgcctgc acagttggac gaactgctga ccgacggtct tgacgctcat
      541 gagaggctcc ttcccaaaaa aggaacgcga gtggttggac cggagtgaac tggaacctcg
      601 cacagtaggg gtcgtcgtcg gccgtagtca aattgctgag gctgggaatg aagaagctcc
      661 acgtgtgcaa acggaaaagc ccggcatgta catgagatgt acggggctcc ggtgtgcttg
      721 agtggtacgc gatcgtgcgc gtctggtcac gcgctcacgc ggcgagtgtg gcgccgcgaa
      781 tctcgtgatg aatcgcgctc ttgttgttcg tgatgacgac tgtttcccac aggtcgatgt
      841 tgtccagctt atagcgatag tcgatgcaca caccggtctg gcggatcagg tcttgcagcg
      901 caaggtcact gcgccagccc agcttcgtgc acagggggca gagccatttc tcgaccatcg
      961 cgatgaagcg gttggtcgat tggaaatggt tgacgatgac gatccgcgcg ccttctttgg
     1021 cgacgcgctg cgcttcctgc acgagcttga acggatcgct gacaacgctg atcacgtggc
     1081 tgatgaagac gtggtcaaaa ctgttgtcgg cgaacggcag cttcatcgcg tcggcttgga
     1141 agacggtggc gtggtcgagg ccgcgctcgc gaatcttctt gcgggcttcg cgcaacatgc
     1201 cggccgacag atcgatgccg aagatccgcc cgcggttggg atagtagttc agcgacacac
     1261 cggtaccgat gcccaagtcc agcacgacat cggtgtcgga gatgttcatg tgctcgatcg
     1321 cgcgctcgat gcggcgcttg accagccggc cgaacgtggc gtcgtagaac accgaatgga
     1381 tatcgtagat cttgcgggtg ctggattcct gcatcagcat cggcggacgt tccttccctt
     1441 tcgcggcggc agaccccggg tgtttaacag ggccgccact ttagtgcccg gcaccacctt
     1501 tgccaagatc ggatttctgg cgggacttat tcttctccgt gcatgcctgc cgcggcggcg
     1561 tcgagcagcc acgtcagttg cccggccggc tcaatcccct gaatcggcag ccgctgaatg
     1621 tcggtctgtc cttccagcac ttccgtcaac cgctgggctt tggcggcgcc cgccaccaag
     1681 ataagcactt ggttcgcctt gttcagaaac ggataggttg tcgtgatccg ccacgcgtta
     1741 agcttcggca cgtgattggc aacgcagcgg tgcttcgcct cgctcagcgc cgccgttccg
     1801 gggaacagcg atgcggtatg cccgtcgtcg cccatgccga gcagaaccat atccgccccg
     1861 ccgtcaccaa acttatcctt cagcagcttg ccatactccg tcgccgccag ttccggcggc
     1921 agctcgccgt gcacgcggtg gatgttgggt tcagggatcg gcagcttcga cagcatcgcc
     1981 tcgtgggcca tgcgatagtt gctctcggga tgatcgggtg gcacgcagcg ctcgtcgccg
     2041 aagtagatct cgaccttcga ccagttgatc tgcgaccgat acggctccgc cgccagggtt
     2101 tcgtacagcg ccttgggcgt cgatccaccg gaaaggacaa gcgcgaagaa atgctgcagg
     2161 tccgccagct tctcctccgc tgccgcaatg atgcggcgag cggcgtcatc cgtgagttcc
     2221 tgcggcgtcg ggaacacttt gatttggcgt gggggcattt gaaactggtc cgatccgccg
     2281 cttgcggctt agcgtctttt ataatgcgag aaacagacgc taagccgcaa gcggcgattg
     2341 catcaggttt ctgctacatt agaattgatc atacctccaa ccaaggacgg cgtggaatat
     2401 cgtgtcgagc ttgacgtttt caacgggccc ctcgatctgc tgctgtacct catcaagcgc
     2461 gacgagctgg acatctacga tattcccatc gcccgcatca ccgaatcgta catgcactac
     2521 gtcgcgatgc taaaggacct caggagcgac gcggggttcg acatcaacgt ggccggggat
     2581 tttctcgtga tggcggcgac gttgatggag atcaagtcgg caacgctgct gccgcgcgct
     2641 cccgtggtga acgaaaccgg cgagccgacg gcggctcagg aattggccga cccgcggtac
     2701 gagctggtta agaagctgtt ggaatacaag gcattcaaag acgccgccgg cgcgctggag
     2761 cggcagcagt attcgcacga ggcgcgcttc ccgcgttttc cggcgaagct ggacggcgag
     2821 gacgcggacg aaccaccgcc ggtggatttg gaagaggtgc aggtgtggga cctgctgtcg
     2881 gcgttcgacc ggctgatgaa agaggtcggg gttcgcaagc acaagtatca cgaggtcacc
     2941 tacgacgaca cgccgatcga cctgcacgcg gcggatatcg aagaccggct gaaacgtgaa
     3001 ggccgattgt cgctgcgcgg gctggtcgtc ggccgcaaga gccgaagcga aatgatcggc
     3061 gtgtttctgg cgctgcttga gctgatccgc gagaagaaga tcctggtgca ccaggacgcg
     3121 ccgctggatg acgtactgat cgaaccggcg ccggaagaac atcggcggac gtacgtgggg
     3181 gcgtcactgc acctgtcgga tgaggcggaa aagacaccga cggatgatga cgccgcggtc
     3241 acatgaattt tctgccgcgc agcggtagcc ccgca
//
LOCUS       DATIMH010000630         4358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1888023, whole genome shotgun sequence.
ACCESSION   DATIMH010000630 DATIMH010000000
VERSION     DATIMH010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888023"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VLI90_10865"
     CDS             complement(<1..861)
                     /locus_tag="VLI90_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14753.1"
                     /translation="MGIYRHVIRPVLFRASPEWIHDRAIGTAAVLGRARAICRITSAV
                     HATDDPRLATEIAGLRFTNPIGLAAGFDKSGRAISMLASLGFGHVEIGSISAEPSAGN
                     PKPRLFRIPRDGGIVVNYGLPNDGAERIAARLVATRKLPVPLGINVVNTNRGPAAPPD
                     SDDPIINDYVQTIRRLRDFADYFSLNLSCPNTKDGRGFFGDRSHLQRLLDLLRDLDLK
                     QPVFLKVSGFPGITEMESFLATVDAAPFIAGFGINLAPGKPPGLTTPAERLKRMPGAV
                     SGRPCAAATDR"
     gene            complement(1093..1932)
                     /locus_tag="VLI90_10870"
     CDS             complement(1093..1932)
                     /locus_tag="VLI90_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV14754.1"
                     /translation="MGRTALVVEDEPLLSEMLVGILDKRGFDATPMLDGTDAAAWVRE
                     HQPDLVMLDLMLPGRSGYEICQELKLDRATNLIPIVICTARTQHEDRLRGLRIGANYY
                     LTKPFTIDQLEHAVNHALAWRREVEEAGTAGEVHFELKSDTHYLEELNRLLSSLFLHT
                     GMSEDDIFQLTTAVREMGSNAIEWGNQKRDDSAVTVTYRIDAEKIVIVIRDEGPGFDR
                     ANLPHAVKGIHDPASHLEVRDAMGLRIGGFGIFMSRGMVDDLQYNHTGNEVRLTKRFA
                     RRD"
     gene            complement(2356..3675)
                     /locus_tag="VLI90_10875"
     CDS             complement(2356..3675)
                     /locus_tag="VLI90_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14755.1"
                     /translation="MMHRICFAVVVTIGLSTVGCVSESNVNSAQARKSPATQPAVLAF
                     PGAQGFGARATGGRGDGGGGSVYHVKNLDDAGPGSLRDAVSQPHRTVVFDVGGYIHLK
                     SVLEVSSDITIAGQTAPGDGIGTLGYEVSFSHQQNVIVRYMRFRQGATPGQEKKYAVG
                     VYQARNMIFDHCSIEWGRWDCLGVNESEDVTFQYCIIGQGISPQRFGALVQSRNITFS
                     HNLWINNESRNPKAKGTVQYVNNVIYNWGKGGGFVEGHSEHDSFDDVVNNVFLAGPNS
                     GHRGAFAQGKPTDKIYSSGNYIDLNANGKWDGTTATDADLGEGTAVRTPFSSLQGLTI
                     EPAETAFRTVLASAGCSLHRDSVDTRLIEQAASLGTAGKIAMNEGEAGGPGELHGGVP
                     APDSDGDGMPDDYERAHGLNPNDAGDAQKLGADGHTALEDYLNGLAR"
     gene            complement(3701..>4358)
                     /locus_tag="VLI90_10880"
     CDS             complement(3701..>4358)
                     /locus_tag="VLI90_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14756.1"
                     /translation="AFRYNVNARFPFWNYICKDAGLAYSVENTTNIGRLYSTGYMKSG
                     QVAYCPANLTDPDFGYESFPKPWLQDTTSIYRSSYAYNTYCNLQSGGTAPNQNRLPAF
                     PKLLTFPKTRILAHDTIDTSGNVMHLGRKKYPSWNCLFKDGHVVNVISPILYAQYNNP
                     AQTAVGKTVNKSYDGYDLLLDILETQASGFNLKTTQLTLRVVHSVGETNGGTTRYHPQ
                     "
BASE COUNT          776 a         1342 c         1380 g          860 t
ORIGIN      
        1 tcgatccgtg gcggccgcgc acggtcggcc ggagacggcg ccgggcattc gcttcagtcg
       61 ctccgctggc gtcgtcagtc ccggtggttt gcccggggcg aggttgatgc cgaagccggc
      121 gataaacggg gcggcatcga cggtggcgag gaacgattcc atctcggtga taccgggaaa
      181 tcccgatacc ttcaaaaaca ccggctgctt caggtcgaga tcgcgcagca aatcgagcaa
      241 ccgttgaaga tgcgatcgat cgccgaagaa cccccggccg tccttggtgt tggggcagct
      301 gaggttgagc gaaaaataat ccgcgaagtc gcgcagccgt cgaatggtct gcacgtagtc
      361 attgatgatc gggtcatccg aatccggcgg cgcggccggc ccgcgattgg tgttaacgac
      421 attgatgccc agcggcaccg gtagctttcg cgtggccacc aggcgcgcgg cgatgcgctc
      481 ggcgccgtcg ttgggcagac cgtaattgac gacgatgccg ccatcgcggg gaatgcggaa
      541 cagccggggc ttcggatttc cggccgaggg ttccgcggag atcgagccaa tctccacgtg
      601 accgaatccc agcgacgcca gcatcgagat cgcccgcccg ctcttgtcga acccggcggc
      661 caggccgatc ggattagtga agcgcaggcc ggcgatctcc gtggccagcc gaggatcatc
      721 ggttgcatga actgcgctgg tgatgcggca gatggcccgc gcgcggccga gcaccgcggc
      781 ggtgccgatg gcgcgatcgt ggatccattc gggactagcg cggaacagca cggggcggat
      841 gacgtggcgg tagatcccca tggcggagat gttatcagat gtgcgccgcc gcgcgccacg
      901 ccaaacccat gtggtcagtg cgcgctccgg tcccctctcc cggtacgccg ggagagggtt
      961 agggtgaggg tgattttcga atgacgaatg acggagcccg tgcgcgaggt gccggtggac
     1021 ggcgatgagc tgctggtctc gcaggagacg cggtgaacag atgcgcgcgc tcgcgctaag
     1081 ccgcaagcgg cttcagtcgc ggcgggcgaa gcgcttggtc agccgcactt cgttgccggt
     1141 gtggttgtac tgcaggtcgt ccaccatccc gcgtgacatg aagatgccga acccgccgat
     1201 gcgcaggccc atcgcatcgc ggacttccag gtgcgacgcc ggatcgtgaa ttcccttcac
     1261 cgcgtgcggc aggttcgccc ggtcgaatcc cgggccttca tcgcgaatca cgatcacaat
     1321 cttttccgcg tcgatgcgat aggtcaccgt gacggcggag tcatcgcgct tctggttgcc
     1381 ccactcgatc gcattcgacc ccatctcgcg gacggccgtc gtgagctgaa agatgtcatc
     1441 ctcgctcatg cccgtgtgaa ggaacagcga cgacaacagc cggttcaatt cttccagata
     1501 atgcgtgtcg ctcttcagct cgaagtgcac ttccccggcg gtgcccgctt cctccacctc
     1561 gcgccgccag gcgagcgcgt gattgaccgc gtgttccagc tgatcgatcg tgaacggttt
     1621 ggtgagatag tagttcgcgc cgatgcgcaa gccgcgcaga cggtcttcat gctgcgtgcg
     1681 cgcggtgcag atgacgatcg gaatcagatt agtcgcgcgg tcgagcttca gctcctggca
     1741 gatctcatac ccgctgcgcc ccggcaacat caggtcgagc atcaccaaat ccggctgatg
     1801 ctcccgcacc cacgccgccg cgtcggtgcc gtcgagcatg ggcgtggcat cgaagccccg
     1861 cttgtccaag atgccgacaa gcatctccga cagcaggggt tcatcctcga ccaccagagc
     1921 cgtccggccc atgcgcgacc tccacccgaa cagtgcgccc cgccgaacgg cgattcttcg
     1981 gcctgaccat cacccggtca agccgacacc aatcccgcgg cgggctctgc gtcacctgca
     2041 agtgatacac ctgctcaaga tacttcctgc gccgccccat tcacaatgta aaccggctgg
     2101 aggttctacg taggcccgac tcttttgcaa tcggcggaca gcagcgagat cgacgtaaat
     2161 tacgtgtccg agccatcgtc gatggcgatc gttgggctgg gcgcgattgg attgctgaag
     2221 cgccggcggc gggattgccg gatcagatga gcctctcatc ccttggaagc aatttcagag
     2281 cctttatcgt gacacgggcc tccggccccg tgtcacggtg agcttcatga cgctctgaaa
     2341 ccatttcttt agccgctagc gcgcgaggcc attcagataa tcttcaagag cggtgtgccc
     2401 atccgcgccg agcttttgcg cgtcgcccgc gtcattggga ttgaggccgt gtgcgcgttc
     2461 gtaatcgtcg ggcatgccat cgccatcgct gtcaggcgcg ggaacaccgc cgtgcagttc
     2521 accggggccg ccggcttcgc cctcgttcat ggcaatcttc cccgcggtgc cgagggatgc
     2581 tgcctgctcg atcaggcgcg tatcgacgga atcacgatgc aaggagcagc cggcgctggc
     2641 gaggacggtc ctgaatgccg tctccgcggg ttcaatcgtc aatccctgca gtgacgagaa
     2701 tggtgtccgc accgcggtgc cttcgcccaa gtctgcgtcc gtcgccgtcg tgccgtccca
     2761 tttgccgttg gcgttcaggt cgatgtagtt gccgctcgag taaatcttgt cggtcggttt
     2821 gccctgcgcg aacgccccgc ggtggccgga gttgggccca gctagaaaaa cgttgttcac
     2881 cacgtcatcg aacgaatcgt gttcggagtg cccttccacg aacccgccgc ctttgcccca
     2941 gttgtagatc acgttgttca cgtactgcac cgtccccttg gccttcgggt tgcggctttc
     3001 gttgttgatc cacaggttgt ggctgaacgt gatgttcctg ctctgcacca gcgcaccaaa
     3061 tcgctgcggc gagatgccct ggccgatgat gcaatattgg aacgtgacgt cttcgctttc
     3121 attcacgccg aggcaatccc agcggcccca ctcgatcgag cagtgatcga agatcatgtt
     3181 gcgggcctgg tacaccccta ccgcgtattt cttttcctgc cccggcgtgg cgccctggcg
     3241 gaagcgcatg tagcggacga tcacgttctg ctggtgcgag aacgacactt cgtaaccgag
     3301 cgtgccgatg ccgtcgcccg gcgcggtttg cccggcgatc gtgatgtcgc tgctgacttc
     3361 aaggacggac ttgagatgga tatacccgcc gacgtcgaag acgacggtgc gatgcggctg
     3421 gctgaccgcg tcgcgcagcg acccgggacc ggcgtcgtcc agatttttaa cgtgataaac
     3481 gctgcccccg cccccgtccc cgcggccacc ggtggcgcgg gcgccgaatc cctgggcccc
     3541 gggaaacgcg agcaccgccg gctgcgtggc gggcgattta cgcgcctgcg cggaatttac
     3601 gttcgattcc gaaacgcagc caacggtgga taaaccaatc gtaacaacaa cggcgaaaca
     3661 aattcggtgc atcatcgaac actcatacac acgaaccggc tcattgtgga tggtagcgcg
     3721 tcgtgccgcc gttggtttcg ccgacgctgt gaaccacacg cagcgtcagt tgggtggtct
     3781 tcaaattgaa accgcttgcc tgggtttcaa ggatatcgag cagaagatcg taaccatcgt
     3841 agctcttgtt gacggtcttg ccgacggcgg tttgcgccgg gttgttgtat tgcgcgtaga
     3901 gaatcgggct gatgacgttg acgacgtgac cgtccttgaa caggcagttc cacgacgggt
     3961 acttcttgcg gcccagatgc atgacgttgc cgctggtgtc gatcgtgtca tgcgccagga
     4021 tgcgcgtctt ggggaacgtc agcagcttgg ggaacgccgg caggcggttc tggttcgggg
     4081 cggtgccgcc ggattgaaga ttgcagtagg tgttgtaggc gtacgagctg cggtagatcg
     4141 aggtcgtatc ctgcaaccac ggcttgggga agctttcgta accgaaatcc ggatcggtga
     4201 ggttcgccgg gcaataagcg acctgcccgc tcttcatgta accggtcgag tagagccggc
     4261 cgatgttcgt ggtgttctcg accgaatatg ccaagcccgc gtccttgcag atgtaattcc
     4321 agaacggaaa gcgcgcgttg acgttgtagc gaaacgcg
//
LOCUS       DATIMH010000631         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5064732, whole genome shotgun sequence.
ACCESSION   DATIMH010000631 DATIMH010000000
VERSION     DATIMH010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5064732"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VLI90_10885"
     CDS             <1..338
                     /locus_tag="VLI90_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSV14757.1"
                     /translation="EHQSAAYERVRAMTRRRDRVLVDTGPLVAILSESDQHHEACVEV
                     LRHIRAPLLTCWPVITEAAWLLRAKPRNVQRLLKSIQDDFIQLLSMESASIEPVADLM
                     RATSGKARS"
     gene            complement(484..1407)
                     /locus_tag="VLI90_10890"
     CDS             complement(484..1407)
                     /locus_tag="VLI90_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSV14758.1"
                     /translation="MLMQSAAVTTAATDRPPPLPRTISRRRLFKRFAAYAAVPVVGGV
                     YATQIEPFWLDPHEVTIPVPGLPAAFQNYRIAHLSDLHAGRTPWTYLQRVVERVRALK
                     PDAVAVSGDSIHHNVNWAEPTSRLLGSLVSNDVPVVVSFGNHEYGVERGDEDPDTPDL
                     ADRFAAALTQQGCTVLINGATAVGRRGQQLWFVGMDDLWFGDFNPTKAFAAVPLGATT
                     IALSHNPDTAEAVDHHRPGLILAGHTHGGQVRLPGFGALQLNTRNTQLDQGLFHLPLG
                     SPLYVSRGVGYIERVRFYCRPELPVFRLLRA"
     gene            complement(1462..3033)
                     /locus_tag="VLI90_10895"
     CDS             complement(1462..3033)
                     /locus_tag="VLI90_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14759.1"
                     /translation="MSQTTLPDSDRSTAAAYRDEAPLSRRRRRTGIVVVLIVSLLASA
                     GTRYWSDEKRAVAIPRRGETAQGSSLSSMNSFALGLLLGGLRGPLVMFLWTQSESQKA
                     DRNLEGIDTQIEWIRLLQPEFDTVHIFQIWNKAYNLSVQMASLTNKYSVILDALKYAH
                     DVDREKPDDINIVTAIAQLYFDKLGGSTEKLYYRRRVRQETLPHATVVRPGGRPVALD
                     PILDDQFDLLSQLTRPAPGRERPANLPADQEWNDGADIQYLSKYQPFPDGVSPLALAY
                     NYYKRAEVLMNVGKQRHAQLSEVVIDSRPALALKGWVEEEWEQGRRRELQSYGIVPPE
                     DRGELELNPAGFTLDHPIADRHAAELAAVAYERAARLVPDALAEYTRTISSNPMNLNN
                     YQGHMDELRAEGELLAGDAASLRAQLSPPEARADLLAKAKQAYQHSIYLYELLLLRYY
                     TDVNYLQQFAPPGYDRFHAADRKGFEDLSAGQMAQLVSNINHAMAARGDTHNEDRQEY
                     ERYMHRMAQRLQHMQ"
BASE COUNT          542 a         1004 c         1031 g          575 t
ORIGIN      
        1 ctgagcacca gtccgcggca tatgaaaggg ttcgggcgat gacccgacgc cgtgaccgtg
       61 tgctggtcga caccggcccg ctggtggcta tcctctccga atccgatcag catcacgagg
      121 cgtgcgtcga ggtgttgcgc cacattcggg cgccgctgct cacgtgctgg cccgtcatca
      181 ccgaggcggc gtggctgctg cgcgccaaac cacgcaacgt tcagcgcctg ctcaagtcga
      241 tccaggacga cttcatccag ctcttgtcca tggaatctgc ttcgatcgaa cccgttgctg
      301 atttgatgcg cgctacgagc ggcaaggcgc gcagttagcc gatgcctgcc tgcttcattt
      361 ggcggagcgc gagcagatcg acaccgtttt caccttggac gtccgcgact tcacagtcta
      421 tcgcatcaag cgaaatcggc ggctgaacct cttaccggca cggcaatatt ttcggtgcaa
      481 agctcaggcg cgaagcaacc gaaacaccgg cagctccggg cggcaataga agcggacgcg
      541 ctcgatgtaa ccgacgccgc ggctgacgta cagcgggctg ccaagtggca ggtggaacaa
      601 accctggtcc agttgcgtgt tgcgggtgtt gagctgaagc gcgccgaagc cgggcaggcg
      661 gacttgcccg ccgtgcgtgt ggccggcgag gatcagtccc gggcgatggt gatcgaccgc
      721 ctcggcggtg tcgggattgt gcgacagcgc gatcgtcgtc gcccccagcg ggacggcggc
      781 gaaggctttg gtggggttga agtcgccgaa ccagaggtcg tccatgccaa cgaaccagag
      841 ctgctgcccg cggcgtccga cggcggtggc gccgttgatc agcaccgtgc agccctgctg
      901 cgtgagggcc gcggcgaagc ggtcggccaa atcaggcgta tccgggtctt cgtcgccgcg
      961 ttcgacgccg tactcgtgat tgccgaagct gacgaccacc ggcacatcat tgctcaccag
     1021 cgatccgagc aaccggctgg tcggctcggc ccagttgacg ttgtggtgaa tcgaatcgcc
     1081 ggacaccgcg acggcgtccg gcttcagcgc ccgcacgcgc tcgacgacgc gctgcaggta
     1141 cgtccacggc gtgcggccgg cgtgcaggtc gctcaggtgc gcgatgcgat agttctggaa
     1201 cgccgccggc aggcccggca ccgggatcgt cacctcgtgc gggtccaacc agaacggctc
     1261 gatctgcgtc gcatagacgc cgcccactac gggcacggcg gcataggcgg caaatcgctt
     1321 gaacaaacgc cggcgggaaa tcgtgcgcgg aagcggcggt gggcgatcgg tcgcggcggt
     1381 agtcacggcg gcggactgca tcagcattgc gctttcatcg gcgcgaaacg actggagcct
     1441 tcattgatcg cgcccgcaga attattgcat gtgctgcagc cgctgcgcca tgcggtgcat
     1501 gtagcgctcg tattcctggc ggtcctcgtt gtgcgtatcg ccacgggcgg ccatcgcatg
     1561 gttgatgttt gaaaccagct gcgccatctg cccggccgac aggtcctcga atcccttgcg
     1621 atcggcggcg tggaaacggt cgtaccccgg cggggcgaat tgctgaagat agttgacgtc
     1681 ggtgtagtag cgcagcagca gcagttcgta caggtagatc gagtgctgat acgcctgctt
     1741 cgccttggcc agcagatccg cgcgagcctc gggcggcgac agctgggcgc gaagcgatgc
     1801 ggcgtcgccg gcgagcaatt cgccttcggc ccgcaactcg tccatgtggc cctgataatt
     1861 gttcaggttc atcggattgc tggagatcgt ccgcgtatat tccgccagcg catcgggcac
     1921 gaggcgcgcg gcccgctcat atgcgacggc cgctaactcg gcggcgtggc gatcggcgat
     1981 cggatggtcc agcgtgaacc ccgcggggtt cagttccagt tcgccgcgat cttcgggcgg
     2041 aacgatgcca tacgactgca gctcgcgccg ccggccctgc tcccactctt cttcgaccca
     2101 ccctttgagc gccaacgccg ggcggctgtc gatcaccact tcgctcagct gcgcgtgccg
     2161 ctgcttgccg acgttcatca gcacctcggc gcgcttgtaa tagttgtatg ccagcgccag
     2221 cggtgaaacc ccatccggga acggctggta cttgctcagg tattgaatgt cagcgccgtc
     2281 gttccactcc tggtccgccg gcaggttcgc cggccgctcg cgaccgggcg ccggccgcgt
     2341 caactgcgac agcagatcga attgatcgtc gaggatcgga tccagcgcca ccggccgacc
     2401 acccggacgg acaaccgtcg cgtgcggcag cgtttcctgc cgcacgcgcc ggcggtagta
     2461 cagcttctcg gtcgagccgc ccagcttgtc gaagtacagc tgcgcgatgg cagtcacgat
     2521 attgatgtca tccggctttt cgcgatccac gtcgtgggcg tacttcagcg cgtcgaggat
     2581 gacgctgtac ttgttggtca ggctcgccat ctggaccgac aggttgtacg ccttgttcca
     2641 gatctgaaag atatggacgg tgtcgaactc cggctggagc aggcgaatcc attcgatctg
     2701 cgtgtcgatg ccttcgaggt tgcgatcggc cttctgcgat tcactttggg tccacaggaa
     2761 catgaccagc ggcccgcgca ggccaccgag gagcaagccc agcgcgaagc tgttcatgct
     2821 gctcagagat gatccttgcg cggtttctcc gcggcgcgga atggcgacgg cacgcttctc
     2881 atcggaccaa tagcgcgtgc cggccgacgc cagcagcgaa acgatcagca caacaacgat
     2941 gccggtgcgc ctgcggcggc gcgagagcgg cgcttcatcg cgataagcgg cggcggtcga
     3001 tcgatccgaa tccggtaagg tggtttggct catggcgcca cctctttgtt cttgaggaag
     3061 acgtacgcca gcaccgccag cggcaagccg aaaccgaata gcacgcccag ggcatcagtc
     3121 acgcgctcgg cgggaatgct gatgccgcgt tc
//
LOCUS       DATIMH010000632         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_732475, whole genome shotgun sequence.
ACCESSION   DATIMH010000632 DATIMH010000000
VERSION     DATIMH010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732475"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1455)
                     /locus_tag="VLI90_10900"
     CDS             complement(94..1455)
                     /locus_tag="VLI90_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSV14760.1"
                     /translation="MKSINPATEEPIAEYPQHTPAQIEKRLRRAESAFDAWRRTRCHD
                     ERTPYMKSAANLLRSRRAELSKLMVREMGKPITGAEQEIDKCADACDYFAEHAEALLA
                     NEPIESDATRSYVRFDPIGPVLAIMPWNFPFWQVIRFAAPNLMAGNVGVLKHSPNVIG
                     CALAIEQLFRDAGFPDGVFTTLLVDTPMVQRIIEHPAIRAVTLTGSERAGRAVAAEAG
                     SVLKKTVLELGGSDPFIVLADADIGAVAKSAAAARCINSGQSCIAAKRFIVEETAADE
                     FEAAFALAMAELVVGDPSKRETQVGPLARRDLMENLADQVTRSVKAGGRVVVGGHQRD
                     KGFYYAPAVLADVRPGMAVFDEETFGPVAAVARAGDEHDLIWLANVTKYGLGASIWTR
                     DLDRAERLAARIEAGNVFVNGPVKSDPRLPFGGIKTSGYGRELGLMGIREFVNAKTVW
                     VKG"
     gene            1799..2827
                     /locus_tag="VLI90_10905"
     CDS             1799..2827
                     /locus_tag="VLI90_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSV14761.1"
                     /translation="MSPLKQRLTLVVLGCAFVGAMGDTVVRAGTVAAGYGAIGDSYAD
                     EYQFYPPDRTHAVNFVEILATQRHLDFGSFSTISRGSPRNQGYANNWALDDATSADLG
                     PQTAGLAGQIAAGQVKTGLVFVGGNDFIHAFLSPNPAQNIPAIPQNLLTNTVTAVSTL
                     LAASPGTNVAVANVFDLSKLPITQQLIATGVLTQAQAGQVSQLISGYNAALAAAFAGN
                     AHVAIIDVNTVANGLVSSPTLKIGGQTIDTQTPGDEFHHLFLNDGLHVGTVGQALIAN
                     GFISALDTQFSANISPLSEGEIIKIAQDAEAAAVPLPPMWASGAITLGCVFAAVNVRR
                     RRRLSHHV"
BASE COUNT          523 a         1005 c          905 g          514 t
ORIGIN      
        1 gcggcggcta aacgacgacg ttcttgtatc gctgatccgt tacttgctcg cgatggcgcc
       61 acaccaattg cgcgcagaac gtccgaaccc atctcacccc ttcacccaaa ccgtcttcgc
      121 attcacgaat tcccggatcc ccatcaggcc aagctcccgc ccgtagccgc tggtcttgat
      181 gccgccgaag ggcaggcgcg ggtcggattt cacggggccg ttgacgaaga cgttgccggc
      241 ttcgatgcgc gctgctaagc gttcggcgcg gtcgagatcg cgcgtccaga tgctcgcgcc
      301 caggccatac ttcgtcacgt tcgccagcca gatcaggtcg tgttcatccc ccgcccgggc
      361 gacggcggcg acggggccaa aggtttcctc gtcaaagacc gccatgcccg gccgcacgtc
      421 ggccagcacg gcgggggcat aatagaatcc cttgtcgcgc tgatgcccgc cgaccaccac
      481 gcgcccgccg gccttcaccg agcgcgtcac ctggtccgcc aggttttcca tcaggtcgcg
      541 ccgcgccagc gggccgactt gcgtttcgcg ctttgatgga tcgccgacga ccagttccgc
      601 catcgccagc gcgaaggcgg cttcgaattc gtcggccgcc gtttcttcga cgatgaaccg
      661 cttggcggcg atgcagcttt gcccgctgtt gatgcaccga gccgctgccg ccgactttgc
      721 caccgcgccg atgtccgcat ccgccagcac gatgaacggg tcggatccac ccagctcgag
      781 caccgtcttc ttcagcaccg accccgcttc ggcggcaacc gcccggccgg cgcgctcgct
      841 gccggtcagc gtgacggcgc ggatcgccgg atgctcgatg atccgctgca ccatcggcgt
      901 atcgaccagc agcgtcgtga acacgccatc gggaaagccc gcgtcgcgaa acagctgctc
      961 gatcgccagg gcgcagccga tgacgttcgg cgaatgcttc agcaccccga cgttgcccgc
     1021 catcaaattc ggcgcggcga accggatcac ctgccagaac ggaaagttcc acggcatgat
     1081 cgccagcacc ggaccgattg gatcgaaccg cacataactg cgcgtcgcat ccgattcgat
     1141 cggctcattg gccagcagcg cctcggcatg ctcggcgaag taatcgcagg catcggcgca
     1201 cttgtcgatc tcctgttcgg cgcccgtgat cggcttgccc atctcgcgga ccatcagctt
     1261 cgacaactcc gcccgccgcg agcgcagcag gttggccgcc gacttcatgt acggcgtgcg
     1321 ctcgtcatgg cagcgcgtgc gccgccatgc gtcaaacgcc gactccgccc gccgcaggcg
     1381 cttctcgatc tgcgccggcg tgtgctgcgg atattcggcg atgggctctt ccgtggccgg
     1441 gttgatggac ttcaacgtcg ctgcgataac ggctcccgtg tcaggtggtg gccaatgata
     1501 tccacatcag gccccccagc taaaaccccg cgcccaagcc cacacaatca gcttttgcct
     1561 gatccgccca ttcgccgggt gatctatagc gcaaatctta atagtctgca aataagatgg
     1621 ttgcagttgc aggagacgat ggaccgcgac gggatggtgg gcaagggata gactggacga
     1681 ccttgaacgt ccggaaatca ttcgacagct tgcccccgag ttattaacat tcccgccgcc
     1741 gtcgccgcag tgggtgctgc tgctgacaaa aggccgtttt gagactgacg aggaaaagat
     1801 gagtccgttg aagcagcgtt tgacgctggt ggtgctgggt tgcgcgttcg tcggagcgat
     1861 gggcgacacc gtcgtccgcg cgggaaccgt ggccgctggc tacggcgcga ttggcgacag
     1921 ttacgccgat gaatatcagt tctatccgcc cgaccgcacg cacgcggtga actttgtcga
     1981 gatcttggcg acgcagcgcc atctcgactt cggctcgttc agcacgatca gccgcgggtc
     2041 gccgcgcaat cagggttacg ccaacaactg ggcgctggac gatgcgacca gcgcggacct
     2101 gggcccgcag acggccggct tggccgggca gatcgccgcg gggcaggtga agacgggatt
     2161 ggtgttcgtt ggcggcaatg atttcatcca cgcgtttctg tcgccgaacc cggcgcagaa
     2221 catcccggcc attccgcaga atttgctgac gaacaccgtc accgccgtca gcacgctgct
     2281 ggccgccagc cccggcacga acgtggccgt cgccaatgtg ttcgacctga gcaagctgcc
     2341 gatcacgcag cagctgattg cgaccggcgt gctcacgcaa gcgcaggcgg ggcaggtcag
     2401 ccagctgatc tccggctaca acgccgcgct cgccgccgcc ttcgcgggca acgcgcacgt
     2461 ggcgatcatc gacgtgaaca ccgtcgccaa cggcctcgtg tcgagcccga cactgaagat
     2521 cgggggccag acgatcgaca cgcaaacgcc cggtgatgaa ttccaccacc tgttcctgaa
     2581 cgacggcctg cacgtcggca cggtcggcca ggcgctgatc gccaacggct tcatcagcgc
     2641 gctcgacacg cagttcagcg cgaacatctc tccgctgagc gaaggcgaga tcatcaagat
     2701 cgcccaggat gccgaagccg ccgcggtgcc gctgcccccg atgtgggcga gcggcgcgat
     2761 cacgctcgga tgcgtgtttg cagcggtcaa tgtgcggcgc cggcgccgcc tgtcgcatca
     2821 tgtgtaatag ccgcgggcga aagccagcgg gggcgtgaga agtcgagcgg cgctttattc
     2881 tcaacccccc gctggctgtc gcctgcggct tcaaaagatc gcgccgtgcg gcgcgcttca
     2941 atcaaaa
//
LOCUS       DATIMH010000633         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3618968, whole genome shotgun sequence.
ACCESSION   DATIMH010000633 DATIMH010000000
VERSION     DATIMH010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3618968"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VLI90_10910"
     CDS             <1..575
                     /locus_tag="VLI90_10910"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HSV14762.1"
                     /translation="QEARANFFTEQLKAVPLADVVVLNESCATTTFTRQRGRCPVHRR
                     LPQHVPAGHWKRLSILAAISAAGVLCAASVDAATDADVFRGFVTDCLVPTLRLGMLVV
                     MDNLSAHKVAGVREAVEASGCRLVYLPPYSPDLSPIENIWSKAKSLMRTLEARTVEDL
                     EVAVTLALEQVTADDCRNCFEACGYSLHLK"
     gene            complement(749..>1557)
                     /locus_tag="VLI90_10915"
     CDS             complement(749..>1557)
                     /locus_tag="VLI90_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV14763.1"
                     /translation="RVMRNADGRSKFKLRHPVAMETTMKWVHRSAARALGLQLAKQSA
                     GLALVALAAALPAASSNAAVINFFDKNSDQTGTGFGHVLEVLDVQNSPSEFGAVLWDG
                     SKDVASGDAKNQGATRTVTELAAKGIDATHFSLIFNINQPKSSDGLDLHNFDLVFQNA
                     AGQTLFTAAFDPNDGANSSTAGLDADGQGTGQSGYEFPVSFTAAEATLFFGTSTNRVG
                     MEILSSHAIDNTTAGGAEGFYVAQAIPEPASGVAMIGIAGAMLLRRARRV"
BASE COUNT          271 a          503 c          484 g          299 t
ORIGIN      
        1 tgcaggaggc ccgcgccaac ttcttcaccg aacaactcaa ggccgtgccg ctggcggacg
       61 tggtcgtgct caacgagagc tgcgcgacga ccaccttcac acggcagcgg ggccgctgcc
      121 cggtccaccg gcgtttgccg cagcacgtcc cggccggtca ttggaagcgg ctgagcatcc
      181 tcgcggccat cagcgccgcc ggcgtgctct gcgcggcgag tgtcgacgcc gccaccgatg
      241 ctgatgtttt ccgcgggttc gtcaccgatt gcctcgtacc gacgctgcgg ctggggatgc
      301 tcgtcgtgat ggacaacctc tcggcccaca aggtcgccgg cgtacgtgag gccgttgaag
      361 cgtccggttg tcggctggtc tacctgccgc cctattcacc ggatctgagt ccgatcgaaa
      421 acatctggtc caaggccaag tcgctgatgc gaacgttgga ggcccgcacc gtggaggact
      481 tggaggtggc cgtgactctg gcgttggaac aggtcacagc cgacgactgc cgaaactgct
      541 tcgaggcgtg cgggtactcg ctacatctga agtgaagatg ctctagcggt gtcggagcga
      601 acgagtctgg actgcggcgc cacccgacaa attcaaatcg ctcgagtctt gtgtgaatct
      661 gttgcgtggt atcaacagaa agatacccac agctccgggc gcgaagtgcc ccagcagggc
      721 gcgcaatcag cactgcacga tcagtccatc acacgcgccg agcgcggcgg agcagcatcg
      781 cgccggcgat cccgatcatc gccacaccgc ttgccggttc cggtatcgct tgggcgacgt
      841 agaacccttc cgctccgccg gcggtggtgt tgtcgatcgc atggctcgaa aggatttcca
      901 tccccacgcg gttcgtgctc gtgccgaaga acagcgtcgc ctcggctgct gtgaagctga
      961 cgggaaattc gtaaccggat tgcccggtgc cctggccgtc tgcatcgagc ccggcggtgg
     1021 aactgttcgc gccatcgttg gggtcgaacg ccgcagtaaa cagcgtctgc cccgcggcgt
     1081 tctggaagac caggtcgaag ttatgcagat cgagcccgtc cgatgatttg ggctggttga
     1141 tattgaagat caaggaaaag tgcgtggcgt cgatgccctt ggccgccaac tcggtgacgg
     1201 tgcgcgtcgc gccctgattc ttcgcgtcac cactggcgac gtccttgctg ccgtcccaca
     1261 gcaccgcgcc gaactcagac gggctgttct gcacatccag cacttccagc acgtgcccga
     1321 atcccgtgcc ggtctggtcg ctgttcttgt cgaagaagtt gatgaccgcg gcgttggaag
     1381 acgcagcggg cagcgccgcc gccagcgcca ccagcgccag gcctgccgat tgttttgcca
     1441 gttgaagtcc caacgcccgc gccgctgatc gatgaaccca cttcatcgtt gtctccatgg
     1501 caaccggatg ccgcaacttg aattttgatc tgccgtcggc gttccgcatc acacgtc
//
LOCUS       DATIMH010000634         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3042191, whole genome shotgun sequence.
ACCESSION   DATIMH010000634 DATIMH010000000
VERSION     DATIMH010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3042191"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..434
                     /locus_tag="VLI90_10920"
     CDS             36..434
                     /locus_tag="VLI90_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14764.1"
                     /translation="MMVVSESFLNRASGQGLHIRRYDVPAGGSVQCTVTGEDDLLIGG
                     LASNLNAAKRVDLSLCDTKGNEQFRLVDIPFDTRSHGVLWQQSITFAKAAPTSTMVAR
                     LIDVRESGEETLLGEYTFNHTRTLPGPAAW"
     gene            complement(479..1168)
                     /locus_tag="VLI90_10925"
     CDS             complement(479..1168)
                     /locus_tag="VLI90_10925"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV14765.1"
                     /translation="MLSVRTKPFVALALLLSLVAPAAPHRAHAQATQPSQPQTQRPTS
                     TPYKGDLSIFEYADRDQKLHVDRVMDLLDIVPGKVVADVGAGSGWFTVRAAARVGPTG
                     QVYAEDISPDAIDYIKKRAARENLANVHTILGEVTDTKLPRSSVDAVLILKTYHEFEH
                     PIPLMTRLRASLRPGAKIGIIDRNGNGTDHGLMPDIVEREMSQAGYHRIGRYSFTKDD
                     GQDYFLIFAAD"
BASE COUNT          287 a          414 c          475 g          324 t
ORIGIN      
        1 agggcgtccg cgcgcttgca cgctctggat cgctcttgat ggtggtgagc gagagctttc
       61 tgaaccgcgc atcaggccag ggcctgcaca ttcgccgata cgatgtgccc gctggcggca
      121 gcgtgcaatg taccgtgact ggcgaagatg atttgcttat cggcggcctg gcttcgaacc
      181 tgaacgcggc gaagcgggtc gacctgagct tgtgtgacac aaagggcaac gagcagttcc
      241 gcctggttga cattccgttc gacaccaggt cgcatggagt tttgtggcag cagtcgatta
      301 cttttgccaa ggccgcaccg acgagcacca tggtcgcgcg gctgatcgac gttcgcgaat
      361 ccggcgagga aacgctgctc ggcgaataca ccttcaacca cacacggacg ctgcctggac
      421 ccgctgcgtg gtaaacaccg gtttccagct cggctcaggt tgaatcgact ggcccggctt
      481 aatcggccgc aaagatcaag aagtagtcct ggccatcgtc tttggtgaag ctgtatcggc
      541 cgatgcggtg gtagccggcc tgcgacattt cgcgctccac aatgtcgggc ataagtccgt
      601 ggtctgtgcc gttgccattg cgatcaatga tgccgatctt tgcgccgggt ctgagcgaag
      661 cgcgaagccg cgtcataagc ggaatgggat gctcgaactc gtgataggtt tttaggatca
      721 gcacggcgtc gacgctggag cgcggcagct tcgtgtcggt cacttcaccc agaattgtgt
      781 gcacgttggc gaggttttct ctggcggcgc gtttcttgat gtagtcgatg gcgtcgggac
      841 tgatgtcctc agcatagact tggcccgtcg ggcccacgcg cgcggccgca cgcactgtga
      901 accagcccga acctgcaccc acgtcggcaa caaccttccc cggcacgata tcaagcaggt
      961 ccatcacgcg atcgacgtga agtttctgat cgcggtcagc atactcgaag atggagagat
     1021 cgcctttgta tggggtgctg gtgggacgct gcgtttgcgg ttgtgatggc tgagtggcct
     1081 gcgcatgcgc tcggtgcgga gcagccggcg cgacgagcga gagcagcagt gcgagagcga
     1141 caaacggttt tgtgcggacg gagagcatga ggaaccccgc gcgagctatc gcgattgtac
     1201 tagggctcaa tgagcaaatt aacgccaatg caagtttcca gcggtagcaa ttctggaaac
     1261 gtggtttggc ctgtaggcaa cagttgagaa ttagttccgc ggattggggg atcgatccgg
     1321 attcgtttgc cgctaatctc ccttggcgca ttgcgttacc aggacgcaat cctgggttcg
     1381 cggcatccgt tgtccgtgcc gattctcttt agcgcatttc gtgctggctg taccgcttga
     1441 caagcgtccc ggaggcttcc aggatatcga ggaaatcgag gccccacccc agaacattgg
//
LOCUS       DATIMH010000635         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5066300, whole genome shotgun sequence.
ACCESSION   DATIMH010000635 DATIMH010000000
VERSION     DATIMH010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5066300"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VLI90_10930"
     CDS             complement(<1..451)
                     /locus_tag="VLI90_10930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HSV14766.1"
                     /translation="MAKRIHVAAVRPGHITLNDAEAHHTRDVLRLKAGDVVEVFDDAG
                     ATASATLSALDSAGVTLDVQQIIEPSRPSGTAITIATAVPKGDRADWMVEKLSELGVA
                     RFIPLITERSVVHPTGKSKSQRWMRLAIESAKQSHRPGVIQIDPLTPL"
     gene            635..1267
                     /locus_tag="VLI90_10935"
     CDS             635..1267
                     /locus_tag="VLI90_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14767.1"
                     /translation="MRTMGQATVDLPDPLNPPTAAPNISAADDLLSQLAGDEIDRLLA
                     DADGGPAMPPPSAPPALDKRPGPAAVDPTAAIDELFAELQAAPVGAAKPAPVEAPATV
                     VAESAPPSPVAEAPAEPIVDEFGPAIAADERAALEQDALDEDSLAGDPLAQLHGVERE
                     LPIVLKPLAWISAPLDRYPEEVRETLGKIAILTIVDALAVLAYVMMFRHH"
     gene            complement(1318..2310)
                     /gene="nadE"
                     /locus_tag="VLI90_10940"
     CDS             complement(1318..2310)
                     /gene="nadE"
                     /locus_tag="VLI90_10940"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HSV14768.1"
                     /translation="MSSDARHGRDARATRVETSSTGIESIYKALLLGLKDYVRKCGFK
                     TVVLGLSGGIDSALTAALAVDALGKDKVVGVAMPSRYSSQHSVDDAKALAEHLGIAFH
                     TIPIQTVHAAYEQTLAPAFAGLQQDVTEENLQARIRGALLMAFSNKFNHLLLTTGNKS
                     ELAVGYCTLYGDMCGGLNLIADVPKTTVYEMAEFLNAKAGRDVIPASTIHKPPSAELR
                     PNQVDQDSLPPYPILDAILHRYVEEEKGAQQIIEEGFDAATVLKVIRLVDRSEYKRRQ
                     APPGLKVTSRAFGFGRRMPIAQRYDQSPPKHGEPSPTIEETKAESGWGYEPAKD"
     gene            complement(2327..3313)
                     /locus_tag="VLI90_10945"
     CDS             complement(2327..3313)
                     /locus_tag="VLI90_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14769.1"
                     /translation="MMNAECRIRKALPLVFFLHSAFIILHSSPAAASVTSALINKALD
                     TQQKLDLDMTLPQAMTQIGNLTGVRIEADPAVWDLLPWGEQTNITAKIENHTLRYALT
                     AITQKLGLEFTLTDEAVELRPMPALRRLGRRSTVQELSALDLLNRTPMNLGGDHPTVR
                     ELLSAVDAKLDAAKSPFAVENRAPDATQDQHIAIARSMSMAEALEEIVRETNATWYPW
                     GSNIVVVPKREQLRTQLGKTLTARYRSVDVGQVLMELFQRAGVDFTVDPGAYQQIPPV
                     YRNITLLLDNATIQQALQTIGGYTGLGFEVNDDGVRVWNQLNPPTTAPTTNP"
BASE COUNT          573 a         1047 c         1123 g          697 t
ORIGIN      
        1 ccagcggcgt cagcggatcg atctgtatca cgcccggccg gtgtgattgc tttgccgatt
       61 cgatggcgag ccgcatccag cgctgcgact tgctcttgcc ggtcggatgc accacgctgc
      121 gttcggtgat cagcgggata aagcgcgcga cgccgagttc gctcagcttt tcgaccatcc
      181 aatcggcgcg atcacccttg ggcacggcgg tggcgatggt gattgcggtg ccgctaggtc
      241 gtgacggttc gatgatttgc tgaacgtcca acgtgacgcc ggcggagtcg agcgcgctca
      301 aggttgcgga agcagtggca ccggcatcgt cgaagacttc gacgacgtcg ccggccttca
      361 gtcgcagcac atcgcgtgtg tggtgggctt cggcatcgtt cagcgtgatg tggcccggtc
      421 gaactgccgc aacgtgaatc cgcttggcca cgcgggcgat tatcagcccg tcgccaatgg
      481 caggcaactt ttcgcgtcga agaattgcga atcgactcgt ggaagttatg atgagcccca
      541 acccgtccga taagggcgtc ggagacgctc actactgccg tcgcgcgtga ttgccctgca
      601 acgcgcgtgc gacgtgcggc catgcgaagg agtcgtgcgc accatgggcc aggcgacggt
      661 tgacctgccc gatccactga acccgcccac cgcggcgccg aacatctcgg ccgccgacga
      721 tctgctttcg caactggccg gcgatgaaat cgatcggctg ttggctgacg cggatggtgg
      781 tccggcgatg ccgccgcctt cggcgccgcc ggctttggat aagcgaccgg ggcccgccgc
      841 ggtcgatccg accgcggcga tcgacgaact gttcgcggag ttacaggcgg ctcctgttgg
      901 cgcggccaag ccagcgccgg tggaagcgcc ggcgacggtc gttgctgaat cagcgccgcc
      961 atcgcccgtt gccgaagcgc cagccgagcc gattgttgat gaatttggtc ccgccattgc
     1021 cgccgatgag cgggcggcgc tggagcagga tgcgctggat gaggattcgc tggcgggcga
     1081 tccgctcgct cagttgcacg gtgtcgagcg cgagctgccg atcgtgctca agccgctcgc
     1141 ctggatcagc gcgccgctgg accgatatcc ggaagaggtg cgcgaaacgc tgggcaaaat
     1201 cgcgatcctc accatcgtgg acgcgctggc cgtgctggcg tacgtcatga tgttccggca
     1261 ccattaagca acgccaataa accgaccact ggtcgcggct atacagtgca gtggcgatca
     1321 atccttcgcc ggttcgtagc cccagccgga ttccgctttg gtttcctcga tcgtcggtga
     1381 tggttcgcca tgcttcggcg gcgactggtc gtatcgctgc gcgatcggca tgcgccggcc
     1441 gaatccgaag gcgcggctcg tcaccttcaa gcccggcggc gcttggcgcc gcttgtactc
     1501 gctgcgatcg accagcctga tcaccttcag caccgtcgcc gcgtcaaaac cttcttcgat
     1561 gatctgctgc gcgccttttt cctcctcgac atagcggtgc aggatcgcgt cgagaatcgg
     1621 atacggcggc agcgaatcct ggtccacctg gttcggccga agctccgccg acggtggctt
     1681 gtgaatcgtg ctggccggga tgacatcgcg gccagcttta gcattgagga actcggccat
     1741 ctcgtacacc gtcgtcttcg gcacgtcggc gatcaggttc aacccgccgc acatgtcgcc
     1801 gtacagcgtg caataaccga cggccagctc gctcttgttg ccggtggtca gcagcaggtg
     1861 gttgaacttg ttactgaacg ccatcagcag cgccccgcgg atgcgcgctt gcaggttttc
     1921 ttccgtgacg tcctgctgca aaccagcgaa cgccggggcg agcgtttgct cgtacgccgc
     1981 gtgcacggtt tggatcggga tggtgtgaaa tgcgatgcca agatgctcgg ccagcgcctt
     2041 ggcgtcatca acgctgtgct gcgagctgta ccggctcggc atcgcaacgc cgacaacctt
     2101 gtctttgccg agggcatcga cagccaatgc cgcggtgagg gccgagtcga tcccaccgga
     2161 gaggccgagg acgacggttt tgaagccgca cttgcgaaca taatctttca ggccgagcag
     2221 cagggctttg tagatggatt cgatgccggt ggaagacgtt tcgaccctcg tagcacgggc
     2281 gtctcgcccg tgcctggcat cgctcgacac gggcgagacg cccgtgctac gggtttgtcg
     2341 tcggcgccgt ggtcggggga ttgagctggt tccacacccg caccccgtcg tcgttcactt
     2401 cgaaaccgag gcccgtgtag ccgccgatgg tttgcagggc ttgctgaatc gtcgcgttgt
     2461 ccagcagcag ggtaatgttg cggtagacgg gcgggatttg ctggtaagcg ccgggatcga
     2521 cggtgaaatc gacgccggct ctttggaata gttccatcaa cacttggccg acgtccacgc
     2581 tgcgatagcg cgcggtcagc gtcttgccca gctgcgtgcg cagctgctca cgcttgggca
     2641 cgacgacgat gttgctgccc cagggatacc acgtcgcgtt ggtctcgcgg acgatctcct
     2701 ccagggcctc agccatcgac atgctgcgcg cgatcgcgat gtgctggtcc tgcgtcgcat
     2761 cgggcgcgcg gttctccacc gcaaacgggc tcttggcggc gtcgagtttt gcatcgacgg
     2821 ctgaaagcaa ttcgcgcacg gtgggatgat cgccgccgag attcatcggc gtgcggttga
     2881 gcaggtcgag ggcggacagt tcctgcacag tcgatcgccg gccgaggcgg cggagcgccg
     2941 gcatcgggcg cagctcgacc gcttcgtcgg tcagtgtaaa ttccagtccc agcttttgcg
     3001 tgatggccgt cagcgcgtag cgcagcgtgt ggttttcgat cttcgcggtg atgttcgtct
     3061 gctcgcccca gggcagcagg tcccaaacgg ccgggtcggc ctcgatgcgc acgccagtca
     3121 ggttgccgat ctgcgtcatc gcctgcggca acgtcatgtc cagatcaagc ttctgctggg
     3181 tgtcgagggc cttgttgatc agcgcggaag tgacggaggc ggcggcgggg gatgaatgca
     3241 gaatgatgaa tgcagaatgc agaaagaaga cgagcggcag cgccttcctg attctgcatt
     3301 ctgcattcat catcagtagt gtccggtcag aagtcgaata gcttcaactg gttgcgattt
     3361 tcgtcttcgg cgattgtttg tccgatctcc aaaaacgcct gatttaccgg cgttttctcg
     3421 aacatcatga cgctgaggat
//
LOCUS       DATIMH010000636         9725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5641790, whole genome shotgun sequence.
ACCESSION   DATIMH010000636 DATIMH010000000
VERSION     DATIMH010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5641790"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /gene="rpsF"
                     /locus_tag="VLI90_10950"
     CDS             complement(<1..521)
                     /gene="rpsF"
                     /locus_tag="VLI90_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSV14770.1"
                     /translation="MPVKASTVNQYEGMFLLGPTGADAEKAVALIRGMIEKHQGQIQV
                     IKRWDERKLAYEIGKQKRGTYVIAFFKATGDNVTTLEREVKLSEDVMRVLITRADHLN
                     EQEMNAVEPQPIAPPPERNPWDRPDGGRGGYDRGPREDRGDRGPREDRAPRGPRREEA
                     PAAAAAAGAAEAGA"
     gene            complement(635..1243)
                     /gene="pth"
                     /locus_tag="VLI90_10955"
     CDS             complement(635..1243)
                     /gene="pth"
                     /locus_tag="VLI90_10955"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSV14771.1"
                     /translation="MKLIVGLGNPGAQYVGTRHNVGFEVVDTLAARLGWILRPEQFDQ
                     LARTKFDGLAIDGQMTTISGGDEKLLLLKPMTFMNVSGKAVQAAMAFYQLAPKDIVVV
                     LDDLALPCGKIRLRPGGSSGGHNGLRDTERVLGTDQYPRLRLGIDPAPPRVPGKDYVL
                     GRFTPEQRKLLDPAIVRAASAIVTWIDKGIELAMSQFNAEIT"
     gene            complement(1345..2007)
                     /locus_tag="VLI90_10960"
     CDS             complement(1345..2007)
                     /locus_tag="VLI90_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HSV14772.1"
                     /translation="MATQSANVTTEPRTQLGSRANKRLRDSGKLPGVIYGHKEAVVPI
                     TLPKKEVVNHLERGTHLFALQVDGKNENVLVKDVQYDHLGLEVIHVDFARVDLNERVE
                     VTVPLELKGEPKGEEEGGVLQQIVAELEIECLVTDIPHSIIHNVSDMALDSVLHVKDL
                     KLPPGVKAMQDEDLIVATVKEIAEEAPTEAVEGETAEPEIIGKKPEEGEEAAAAAGEE
                     KK"
     gene            complement(2094..3065)
                     /locus_tag="VLI90_10965"
     CDS             complement(2094..3065)
                     /locus_tag="VLI90_10965"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HSV14773.1"
                     /translation="MQSDADKIKLFTGRANPQLAQKIADYLELPLGRGRTELFPDGEL
                     IVKVEEDVRGRDCFIIQPTSHPINAHLMELFIWIDCLKRASAARVTAVIPYFGYARQD
                     RKDEGRTPITAKLVANLLERSGADRVVSVDLHAAQVQGFFDIPVDHLSASPVISKWFK
                     SLKLTNRVFVSPDVGNVKRAQKYANALGGEICIIDKRRKSGKDTVAKNIIGDVEGKNV
                     LMVDDMVTTAGTMTEAVRILRDNGALDIYIAATHAVFAPPAMERLQQCKFTKIAVTDT
                     IPINDRADAIKDRLVVLSTAQLLGEAIHRIHHNASVSELFRDDGKKE"
     gene            complement(3151..4137)
                     /locus_tag="VLI90_10970"
     CDS             complement(3151..4137)
                     /locus_tag="VLI90_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HSV14774.1"
                     /translation="MSASAIILAAGKSTRMKSRRPKPLHDVCGRPMLEYVLRACYEAG
                     CDRVVVVVGHGKDEIVSQFGGDKRIHWVEQTEQLGTGHAARMCEAELKQHGGDVFILT
                     GDGPLIRGEVLQTLLRAHREEKAAASMATAVLDDPTGYGRIVRDENGEFVEIVEQSDA
                     TEEQREIREVFPSYYCVRSEELLLALSKLTNNNKKREYYLTDIYAILRNAGKKVLAVQ
                     AVTQEDSLAVNDRIQQAEVDAIMQDRIQKQLRSEGVTIVSSINTYIEDGAAIGPDTIV
                     HPFTFIGRDSSIGAECIIGPFATLPRESIVPEGTTVAGNVTAENATLPEGGG"
     gene            complement(4171..4575)
                     /locus_tag="VLI90_10975"
     CDS             complement(4171..4575)
                     /locus_tag="VLI90_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14775.1"
                     /translation="MRISSWMIAAAMLAGLTTALVGCQEHKPQPRQTPAQLQDRLANA
                     RGIGSNDVRDQALHDIAVDAAASRVGDVAVTATSSIGNAKMRDDTAEQAAKILDDHSD
                     RASAERVVQLMSDSSRRDAMRSYLASRAPSAR"
     gene            complement(4637..4707)
                     /locus_tag="VLI90_10980"
     tRNA            complement(4637..4707)
                     /locus_tag="VLI90_10980"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4673..4675),aa:Gln,seq:ttg)
     gene            5125..7290
                     /locus_tag="VLI90_10985"
     CDS             5125..7290
                     /locus_tag="VLI90_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HSV14776.1"
                     /translation="MHIKITRFACLCLLLSLMLSGFTQAAPPLADQAPADTWVYVGWA
                     GVDALQSAYEQSNLGAVVKQSNLPQLLDQHITMFWSSVSGPGRRGGAEDPQVAAARQI
                     VSILWHHPTSFYWQAMPTPIATPTYPRAGLVCDAGADAAALTQLFRGLGPRAFGPGTR
                     VIANGTIIMMTAGKPAPGGLVADPAFTAAMPHVQSSPAICIYANVTGALEQINQLAAT
                     DHDSAVFWPKVRDALGLNNIKTYARSAGFEGADWGTAAFLAVPSPRAGVLTAIEPHAM
                     DPALLARIPVNANSFSVLDFDAGKLVDTIHEAFVASSQEKAFNMGTGAISVMLGRNLR
                     RQLLAPLGGQWVMYSTPDSHGPVLLNKVTDEKTEQDALVSALYGISNLVNTQFGSRGG
                     PPIVTASQSQSGDLTVTTATVKEFAPSFAIRNGIVYFGMSPDAVTAAATMADVTGSTV
                     ATQRDVFTAARQRLGVSGDLTGNFSFANLPETAAGAYKSLGQESKLVHDLAVSSNIPW
                     PEIDLPTFEELKPHLSPAVSLRWADDAGIYTKSISPFPLSGALLGDSQSAVMSSGAVA
                     AIASVVAPALARGRATSQRVQCASNERHILMGIIQYTNDHNGEYPPDLGSLMKYINSP
                     VVFVCASAGTVVPPEILLAPKDKQAAWVNENTDYIYHGKGLSTAKSHAMTPVVSERPT
                     DHGPDGMNVGFADGHVEWVNAAQAKKMLNTAPDAGEQGL"
     gene            7435..9627
                     /locus_tag="VLI90_10990"
     CDS             7435..9627
                     /locus_tag="VLI90_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14777.1"
                     /translation="MRTRLITICISILFAATSAPIQAAPLAERIPADALIYIGWTGSD
                     SLGADYQQSHLKAVLDVSAIPELCQTFLPELIQRVGPRDRQAARVMQLIQAIGGPMWR
                     HPSAFYFGGIDWSGPQPTPRLALMCDAGAEADKLVATANQLLARAPRGGPPITVRRDA
                     SLVMLMMGPPAMGQALSHPLTDSLAKSAAFTAALAQAHGNDAVAAGYVDVQGIVKTVD
                     ESLQRIGEQNATQLWTKAREALGLSGMRQAAWSAGFDGKDWNTEGFVGTDGGKAGLLA
                     MFNAPPLSDDALAMVPQSANRVAAGHFDLGALVRQIRAGIEQFDPQTAEQFDAGLARA
                     NMISGVDLQKDLIDPLGDQWVLYSDRNIGGPGLLGAVVVNKLKDPARTETALASLAQR
                     GNDMIAGAMGNKDEMTVQFRETTVDGLSVHYLAVPLISPAWAVKDGNLYLALYPQVIS
                     SAARFASSHGPSITSNTAYIATQKRLGDHKPSGVSFSNLPATAGDGYQDLLAISRLYL
                     GFSDMLGVPTPAMVLPPLDKLLPHVTPAGGMSWTDDAGWHFKSIQPFPGSDALADNGF
                     GAMMVGQDALLISILLPSLNRARETANRVKCANNERQMGQGLLLYANDNKGKLPDDLG
                     TLVKTEDITPEVFLCPSRGQSVPPEVAQMTPDQKVAWVNEHANYVYVGKGQTSSAPAD
                     RIILYEKPDAHEGDGMNILYGDGHVEFQRTEDANRAIEKQEKRGEQGL"
BASE COUNT         1663 a         3172 c         3003 g         1887 t
ORIGIN      
        1 gcgccagctt cggcggcgcc cgcggcggcg gcggcggcgg gggcttcctc gcgacgcgga
       61 ccgcgcgggg cgcgatcttc gcgcggacca cggtcgccgc ggtcttcgcg cgggccgcga
      121 tcgtaaccac cgcggccacc gtcggggcgg tcccacgggt tgcgctccgg cggcggggcg
      181 atcggctgcg gttccaccgc gttcatctcc tgctcgttga gatgatccgc ccgcgtgatc
      241 agcacgcgca tcacgtcttc gctgagcttc acttcgcgct ccagcgtggt gacgttgtca
      301 ccggtggctt tgaagaaggc gatgacgtag gtcccgcgct tctgcttgcc gatctcgtaa
      361 gcgagcttgc gctcgtccca gcgcttgatc acctggattt ggccctggtg cttttcgatc
      421 atgccgcgga tcagcgcgac ggccttctcc gcgtcggcgc cggtcgggcc gagcaggaac
      481 atgccttcgt actggtttac tgtcgatgct ttgactggca taaatgcaaa ctccctagct
      541 tgggttttgg ccggcttggt gtccggtcgc tagttttgta gcacgggcgt ctcgcccgtg
      601 tcgcagacca ttcggcatga gcaagatacc catgctacgt tatctcggcg ttgaactggc
      661 tcatcgccaa ttcgattcct ttgtcaatcc acgtcacgat cgcgctggcc gctcggacga
      721 tcgccgggtc cagcagtttt cgctgctccg gtgtgaatcg gccgagcaca taatccttcc
      781 ccggcacacg cggcggggcg gggtcgatgc ccagcctgag ccgcgggtac tggtccgtgc
      841 cgagcacgcg ctcggtgtcg cgcagaccgt tatgaccacc gctgctgcca ccgggccgca
      901 ggcggatctt gccgcagggc agcgccaggt cgtccaggac gacgacaatg tccttcggcg
      961 ccagctggta aaacgccatc gccgcctgca ccgctttgcc gctaacgttc atgaacgtca
     1021 tgggcttgag cagcagcagc ttttcgtcgc cgccgctgat cgtcgtcatc tgcccgtcga
     1081 tggccagtcc atcgaatttc gtgcgggcca gctgatcgaa ctgctcgggc cgcaggatcc
     1141 aaccgagtcg ggcggccagc gtgtcgacca cttcgaaccc gacgttgtgc cgggtgccga
     1201 cgtactgcgc gcccgggttg cctaggccga cgatgagctt catggcgttt gtccgttgtc
     1261 agtcgttcgt tgtcagttgt tggtgacgct gccttgcgaa tcacgccgcc acaactgatc
     1321 actgacaact gaccactgac aaaactactt cttctcctcg cccgcggcgg ccgcggcttc
     1381 ttcgccctct tccggcttct tgccgatgat ttcgggctcg gcagtttcgc cttcgacggc
     1441 ttcggtcggg gcttcctcgg cgatttcctt cacagtcgcc acgatcaggt cctcatcctg
     1501 catcgccttc acgcccggcg gcagcttcag gtccttgacg tgcaggacgc tgtccagcgc
     1561 catgtccgag acattgtgga tgatcgaatg cgggatgtcg gtgacgaggc attcgatctc
     1621 caactcggcc acgatctgct gcagcacgcc gccttcttct tcgcccttgg gctcgccctt
     1681 gagctcgagc ggcaccgtca cctcaacgcg ctcgttcaaa tcgacgcggg cgaagtcgac
     1741 gtggatgact tcgagcccga ggtgatcata ctgcacgtcc ttgacgagca cgttttcgtt
     1801 cttgccgtcg acctgcagcg cgaacaggtg ggtgccgcgc tcgaggtgat tgacgacttc
     1861 cttcttgggc agcgtgatcg gtacgacggc ttccttatgc ccgtagatca cgcccggcag
     1921 cttgccgctg tcgcgaaggc gcttgttggc gcgcgatccc agctgggtgc gcggctcggt
     1981 ggttacgttt gcggattggg ttgccatatt cactccagta gcaaggctct gagcctgcgg
     2041 ctctctcgcc cgtgtcgtac gtctctaggc acgggcgaga cgcccgtgct acgtcattcc
     2101 ttcttaccgt cgtccctaaa cagctcgctc accgatgcgt tgtggtggat gcgatggatt
     2161 gcttcgccca gcagctgcgc cgtactcagc accaccagcc gatctttgat cgcgtcggcc
     2221 cgatcgttga tcgggatcgt gtcggtcacc gcgatcttgg tgaacttgca ctgctgcaag
     2281 cgctccatcg ccggcggggc gaacaccgcg tgggtggccg cgatgtagat gtccaacgcg
     2341 ccgttgtcgc gcaggatgcg caccgcttcg gtcatcgtcc cggcggtcgt caccatgtcg
     2401 tccaccatca acacgttctt gccttcaacg tcgccgatga tgttcttcgc aaccgtgtct
     2461 ttgccgctct tgcggcgctt gtcgatgatg cagatctcac cgccgagcgc gttggcgtac
     2521 ttctgggcgc gcttgacgtt gccgacgtcg ggcgacacga acacgcggtt ggtgagctta
     2581 aggctcttga accacttgct gatcaccggc gacgccgaca gatgatcgac cgggatgtcg
     2641 aaaaatccct gcacctgtgc cgcgtgcaga tcgaccgaca ccacgcggtc ggcgccgctg
     2701 cgctcgagca aattcgccac cagcttcgcc gtaattggcg tgcggccttc atccttgcgg
     2761 tcctggcgcg cgtatccgaa gtacggaatc accgccgtca cgcgggcggc gctggcccgc
     2821 ttcaggcagt cgatccagat gaacagctcc atcaagtgag cgttgatcgg atgactggtc
     2881 ggctgaatga tgaagcagtc gcgcccgcgg acatcttctt cgactttgac gatcaactcg
     2941 ccgtcgggaa acaattccgt gcgcccgcgg cccaggggaa gttccaggta atccgcgatc
     3001 ttctgcgcca gctgcgggtt cgcccggccg gtgaagagtt tgatcttgtc cgcgtcggat
     3061 tgcatttgtt gtccgtggtc agtggtccgt tgtcagttgt gttggccgcg ttttaacaac
     3121 ggacaacgga cgactgacaa tggacagacc tcatcctccg ccttccggta acgtcgcgtt
     3181 ctccgccgtc acgttccctg caactgtcgt cccttccgga acgatgctct ctctcggcaa
     3241 cgtcgcaaac ggcccaatga tgcactccgc tccaatgctc gaatcgcggc cgatgaacgt
     3301 gaacggatgc acgatcgtgt ccggcccgat cgccgcgccg tcttcgatgt acgtgttgat
     3361 gctgctgacg atggtcacgc cttcgctgcg cagctgcttt tgaattcggt cctgcatgat
     3421 cgcgtccact tccgcctgct ggatgcggtc gttgacggcc agcgaatctt cctgcgtcac
     3481 cgcctgcacc gcgagcacct ttttgccggc gttccgcagg atcgcataga tgtcggtgag
     3541 gtaatactcc cgtttcttgt tgttgttcgt cagcttcgac agcgccagca gcagctcttc
     3601 ggaccgcacg cagtaatagc tggggaagac ctcgcgaatc tcgcgctgct cctccgttgc
     3661 gtcggactgc tcgacgatct cgacgaactc gccgttctca tcgcgaacga tccggccata
     3721 gccggttgga tcatccagca ccgccgtcgc catgctggcg gcggcttttt cttcgcgatg
     3781 cgcccgcagc aacgtctgca gcacttcgcc gcggatcagc ggcccatcac ccgtcaggat
     3841 gaagacatca ccaccgtgct gcttcagttc cgcctcgcac atgcgggcgg cgtgcccggt
     3901 gccgagctgc tcggtttgtt cgacccagtg aatgcgcttg tcgccgccga actgcgagac
     3961 gatctcgtcc ttgccgtgac cgacgacaac gaccacgcga tcgcatcccg cttcgtagca
     4021 cgcgcgcagg acgtattcga gcatcggccg gccgcacaca tcgtgcagtg gtttgggccg
     4081 gcgcgatttc attcgcgttg acttcccggc ggcgaggatg atggcactgg cgctcatgcg
     4141 tcttgtctcg aaatccgttt cgtcgttcgt ctacctggcc gacggcgcgc gggatgcaag
     4201 gtacgatcgc attgcgtctc gccgggatga atcactcatc agctgcacca cccgttcggc
     4261 gctggcgcgg tcgctgtggt catctagaat cttggccgcc tgctcggcgg tgtcgtcgcg
     4321 catcttggca ttgccgatcg aactggtcgc ggtgaccgct acgtctccca cgcgcgatgc
     4381 ggcggcgtcc acggcaatgt cgtgcagcgc ctggtcgcga acgtcgttgc tgccaatccc
     4441 gcgagcgttc gccaaacgat cctgcaactg cgccggtgtc tgccgcggtt gcggtttgtg
     4501 ttcctggcat cccaccagcg cggtagtcaa cccggccaac atcgccgccg cgatcatcca
     4561 gctcgagatt cgcatcgctt ggctcatcgt tgattcctcg cggcgcccaa ttgcgttccg
     4621 gcaaacggga agacaactac ccggcctgga ttcgaaccag gacaaagaga accaaaatct
     4681 cttgtgctac cgttacacta ccgggtaact gatcgaccct gcgtcatgtc cgaaagcagg
     4741 gcgatggcag tgaaagcgaa gggcaagacg ccgcggcgcg ttgaaacgcc ggctggaatg
     4801 ctgtccgttc gcggtacgcg agccgatcaa tcgtcagctc atcccggagg cccgagtcgg
     4861 ttcaaaccgc gagtccgacc cggcttatct acccatccag ccacgacgag tgtacccact
     4921 cgtgccggac ctctcgacaa acgcccgtca atgcgggata tgcggcacat cctacggcca
     4981 ggacgaaccc tgtcaagcca gccgcctctc gtgcgattgc atctccaagt gatctaagca
     5041 aatgcaatcc tacccccttg aatccgtctg cactgtttag aagattagtt gcgacaacga
     5101 cctactcatc ccgggaagat ttctatgcat atcaaaatca cgcgcttcgc ctgcttgtgc
     5161 ctgctcctgt cgctgatgct ctcgggtttc acccaagccg ctccgccgct ggcggatcaa
     5221 gcgccagcgg atacctgggt ctacgtcgga tgggcgggcg tggatgcgtt gcaatcggcg
     5281 tatgagcagt ccaatctcgg ggcggtggtc aagcaaagca acttgccgca gctgctcgac
     5341 cagcacatta cgatgttctg gtcgagcgtg agcggccccg gccgccgcgg gggggcggaa
     5401 gatccgcagg tggcggcggc ccggcagatc gtgtcgatcc tgtggcatca cccgacatcg
     5461 ttctactggc aagccatgcc gacgcctatc gcgactccga cctatccgcg cgcgggtctg
     5521 gtctgcgacg ccggcgcgga tgcggccgcg ctgacgcagt tgttccgcgg gttggggccg
     5581 cgcgcgttcg gacccggcac gcgcgtcatc gccaatggta ccatcatcat gatgaccgcg
     5641 ggcaaaccgg cgcccggtgg tctggtcgcc gacccggcgt tcacggcggc catgccgcac
     5701 gtgcagtctt cgccggccat ctgcatctac gccaacgtca ccggtgcgct cgagcaaatc
     5761 aatcaactgg cagccactga tcacgactcg gcggttttct ggccgaaggt tcgcgatgcg
     5821 ctggggctga acaacatcaa gacgtacgcg cgcagcgctg ggtttgaagg agcggactgg
     5881 ggcaccgccg cgtttctggc ggtgccgtcg ccgcgcgcgg gcgtattgac cgccattgaa
     5941 ccgcacgcga tggacccagc tctgctggcg cgcatccccg tcaatgcgaa ttcgttttct
     6001 gttttggatt tcgacgctgg aaaattggtg gacacgatcc acgaagcatt cgtcgcctca
     6061 tcccaggaaa aagcgttcaa catgggcacc ggcgccatct cggtgatgct gggccgcaac
     6121 cttcgccggc agctgctcgc gccgctcggc gggcaatggg tcatgtattc aacgccggat
     6181 tcgcacgggc cggtgctcct gaacaaggta accgacgaga agaccgagca ggatgcgctg
     6241 gtcagcgcgc tctacggcat ctcgaaccta gttaacacac agttcggctc acgcggcggg
     6301 ccgccgatcg tcaccgcttc gcaatcccaa tccggtgacc tgacggttac caccgccacc
     6361 gtcaaggaat tcgcgccttc gttcgcgatt aggaacggca tcgtgtactt cggcatgagc
     6421 ccggatgccg tgacggccgc ggcaacaatg gcggatgtga caggatcgac ggtagccacg
     6481 cagcgcgacg tgtttaccgc cgcgcggcag cgcctgggtg tgagcggcga tctcaccggc
     6541 aacttcagct tcgcgaacct gccggagacg gccgccggcg cgtataagtc gctggggcaa
     6601 gagtcaaagt tggtgcacga tttggcggtg tcgagcaaca tcccctggcc ggagatcgat
     6661 cttccgacat tcgaggagtt gaagccgcac ctgtcaccgg cagtgagcct gcgatgggcg
     6721 gacgatgccg gcatttacac caagtccatc agccctttcc cgctgagcgg cgcgctgctc
     6781 ggcgattcgc agtcggcagt gatgtcctca ggcgcggtgg cggccatcgc atccgtcgtc
     6841 gcgccggcgc tggcgcgagg gcgcgccaca tcccagcgcg tccagtgcgc gtcgaacgag
     6901 cggcatatcc tgatgggcat catccagtac accaacgatc acaacggcga atacccgccg
     6961 gacctcggct cgctgatgaa gtacatcaac tcgcccgtcg tcttcgtctg tgccagcgcc
     7021 ggcacggtgg tgccgccgga gatcttgttg gcgcccaagg acaagcaggc agcgtgggtc
     7081 aatgaaaaca ccgattacat ctatcacgga aagggattga gtacggccaa gtcccacgca
     7141 atgacgccgg tcgtcagcga gcgaccgacc gaccacggtc ccgacggaat gaatgtcggc
     7201 ttcgcggacg gccacgtcga atgggtaaat gccgcccagg cgaagaagat gctgaacacc
     7261 gcccccgacg ccggcgagca ggggttgtga gtctgccacg ccgggttcgc aggtactccg
     7321 acgacccgga ttcttcttcg agtcccaaag caatccgggt cgtcgcgagt acgctccgaa
     7381 cccggcgtgg tcggatacga tgccttcgat tagtcttcga ccaggagcac caccatgcgc
     7441 actcgactca tcaccatctg catttcgatt ctgttcgccg ccacatccgc gccaattcag
     7501 gccgcgccgc tcgccgagcg catccccgcc gatgcgctga tctacatcgg ctggaccggc
     7561 tccgattcgc ttggggccga ctaccagcag tcgcacctca aggcggtgct cgacgtctcg
     7621 gcgattcccg aactctgcca aaccttcctc cccgagctga tccagcgcgt cggcccgcgc
     7681 gaccggcagg cggcccgcgt gatgcagctg attcaagcga tcggcgggcc gatgtggcgg
     7741 catccctccg ccttctactt cggcggcatc gactggtccg gcccgcaacc gacgccgcgg
     7801 ttggcgctga tgtgcgacgc cggcgccgaa gccgacaagc tggtggcgac ggcgaaccag
     7861 ctgctcgccc gcgcgccgcg cggcgggccg ccgatcaccg tgcggcgcga tgcttcgctc
     7921 gtcatgctca tgatgggacc accggcaatg gggcaggcgc tgtcgcatcc gctgacggac
     7981 tcgttggcga agtccgcggc gtttaccgca gcgctcgcgc aagcgcacgg caacgacgcg
     8041 gtcgccgccg gttacgtcga tgtgcaaggc atcgtgaaga cggtggacga gtcgctgcag
     8101 cggatcggtg agcagaacgc gacgcagctt tggaccaaag cgcgcgaggc cctcggtctt
     8161 tctggcatgc gccaggcagc atggtcggcg ggctttgatg gcaaggactg gaacaccgaa
     8221 ggcttcgtcg gcacggatgg tggcaaggcc gggttgctcg cgatgttcaa cgcgccgccg
     8281 ctcagcgatg acgcgctggc gatggtgccg cagtccgcca accgcgtggc cgcgggacac
     8341 ttcgacctgg gcgcgctggt ccggcagatc cgcgctggca tcgagcagtt cgatccgcag
     8401 acggccgagc agttcgacgc cggcctcgcc cgcgcgaaca tgatctccgg cgtcgatctt
     8461 cagaaggacc tgatcgaccc gctgggcgat cagtgggtgc tgtacagcga tcgcaacatc
     8521 ggcggccccg gattgctcgg cgcggtcgtc gtcaacaagc tgaaagaccc ggcccgcacg
     8581 gagacggcgc tcgcgtccct cgctcagcgc ggcaacgaca tgatcgccgg cgcgatgggc
     8641 aataaagatg agatgaccgt tcagttcagg gagacgaccg tcgatgggtt gagcgtgcat
     8701 tacctggcgg tcccgctgat ctcgcccgcg tgggcggtga aggatgggaa tctgtatctc
     8761 gcgttatatc cgcaggtgat cagctcggcc gcgcgcttcg ccagcagcca tggcccgtcg
     8821 atcacgagca acaccgcgta tatcgccacg cagaagcggc tgggcgatca caagccaagc
     8881 ggtgtgtcgt tcagcaactt gcccgctacc gcaggcgatg gttaccagga cctgctggcg
     8941 atcagccggt tgtatctcgg attctccgac atgctcggcg tgcccacgcc cgcgatggtg
     9001 ctgccgccgc tggacaagct gctcccgcac gtcacacccg ccggtggaat gtcgtggacc
     9061 gacgatgctg gttggcactt caagtcgatc cagccgttcc ccggcagcga cgcgctggcc
     9121 gacaacggtt tcggcgcgat gatggtcggc caggatgcgc tgctgatcag catcctgctg
     9181 ccgtcgctca accgtgcgcg cgaaaccgcc aaccgagtaa agtgcgcaaa caacgaacgc
     9241 cagatgggcc agggcctgct gctgtacgcc aacgacaaca agggcaagtt gcccgacgat
     9301 cttgggacgc tggtgaagac ggaagacatt acgcccgagg tgttcctatg tccgtcgcgc
     9361 ggccaaagcg tcccgccgga ggtcgcgcag atgacgccgg accagaaggt cgcgtgggtg
     9421 aacgagcacg cgaattacgt ttatgtcggc aaaggccaga cgagttccgc gccggccgat
     9481 cggatcattc tgtacgagaa gccggacgct cacgaaggcg acgggatgaa catcctgtac
     9541 ggcgacgggc acgtagaatt tcaacgcacg gaagacgcga atcgcgcgat cgagaaacag
     9601 gaaaaacggg gggaacaagg actataaacg cgtcccttca caaaagacct acccccgcag
     9661 cgcccggcta cgccaaatgc gcgccggcgc agccgggggc tgcgggggtg acgcttattg
     9721 accaa
//
LOCUS       DATIMH010000637         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3044576, whole genome shotgun sequence.
ACCESSION   DATIMH010000637 DATIMH010000000
VERSION     DATIMH010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3044576"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VLI90_10995"
     CDS             <1..735
                     /locus_tag="VLI90_10995"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HSV14778.1"
                     /translation="SGIAKKGFWQKDVLKGFPSWLERVEVPKKDGTVHHLLVNDARSL
                     LWMANQNTITPHVWVSRVPELYHPDLCVFDLDPPDAKRGDTVRAIALALRALLRELGL
                     ESWIKTTGSKGFHIVLSLDGTSPMGEVAGFAHHVGAMLVTAAPKHVTQEFSKADRGGR
                     ILIDTGRNGYSATFAAAYALRARPGAPVSAPCTWEEIESGTIDPGSVTLRNISDRIAA
                     VGDLWATFAEHRQSLQPAIDRLAKLR"
     gene            859..1254
                     /locus_tag="VLI90_11000"
     CDS             859..1254
                     /locus_tag="VLI90_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV14779.1"
                     /translation="MVRYGRIPVLIVDDEPSIHQLLGRLFGWHRIPTLHASSFATALD
                     LASRHAISAMVLDLRLQAAESGLDLLRWLRASPAYRQLPVCIFTGSVTLNEDEEAAVT
                     YHDAEVFFKGKDLNRLVERIAAAATHRAA"
     gene            1327..>1557
                     /locus_tag="VLI90_11005"
     CDS             1327..>1557
                     /locus_tag="VLI90_11005"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HSV14780.1"
                     /translation="MLRPTMVTSVDQQRYLGCLVGLAVGDALGTTLEFRPKGSFEPIT
                     EMIGGGPFRLDPGQWTDDTSMALCLAESLLEKG"
BASE COUNT          272 a          493 c          504 g          288 t
ORIGIN      
        1 tccggcatcg ccaaaaaagg tttttggcag aaggacgtgc tgaaaggatt tccatcgtgg
       61 ctggagcgcg tggaagtacc gaagaaggat ggcaccgtcc atcatcttct cgtcaacgac
      121 gcccgttcgc tgctgtggat ggccaatcag aacaccatca ccccgcacgt gtgggtgtca
      181 cgcgtgccgg agttgtatca cccggatctg tgcgtgttcg atctcgaccc tcccgatgcg
      241 aaacgcggtg acacggttcg cgcgatcgcg ctcgccttgc gcgcgttgct gcgcgagctt
      301 ggtctcgaga gttggatcaa gacgacgggc tccaaaggat ttcatatcgt gctgtcgctc
      361 gacggcacgt cgccgatggg cgaggtggcg gggttcgcgc atcacgtcgg cgcgatgctc
      421 gtgacggcgg cgccgaagca cgtgacgcag gagttcagca aagccgatcg tggtggacgc
      481 atcctgatcg acaccggccg caacggctac agcgcgacgt ttgccgccgc ctacgcgctt
      541 cgcgcgcggc ccggagcacc ggtctcggca ccgtgcacat gggaggagat cgagtcgggg
      601 acgatcgacc caggcagcgt cacgctgcgc aacatctccg accggatcgc cgccgttggc
      661 gatctgtggg cgacgttcgc ggaacaccgg cagtcgttgc agccggcgat cgaccgtctc
      721 gccaagctgc ggtaaggctg ccggacggcg tcagggtgat cgtgggcatc cgcagtgcac
      781 ttccgcgcat cacgggctcg aaggcccgcc ctgaacgtgc ggcttacgat gtgccggcga
      841 tgtgccgata aggactttat ggttcgctat ggacgcatcc cggtgttgat cgtggatgac
      901 gaaccgtcga ttcatcagct cctcggtcgc ctcttcggat ggcaccgcat tcccacgctc
      961 catgcgagct ccttcgccac ggcgctcgat ctcgcgtcgc gccacgccat cagcgcgatg
     1021 gtgctcgatc tacgactcca ggccgccgaa tccggattgg atctcctgcg gtggcttcgc
     1081 gcgtctccgg cctaccggca gttgcccgtc tgcatcttca ccggcagtgt cacgttgaac
     1141 gaagacgaag aagcggccgt gacgtatcac gacgcggaag tcttcttcaa aggcaaggat
     1201 ctcaatcgcc tcgtcgaacg catcgccgcc gcagcgaccc accgagcggc gtgacccgcg
     1261 cacatactca ctgacgaccg tccggcgcat gggccggtgc gtcgatctcg tacgccgcgg
     1321 cgctgtatgc tgcgacctac gatggtgacc agcgtggatc aacaacggta tctcgggtgc
     1381 ctggtcggcc tggccgtcgg tgatgcgctc ggaaccacgt tggaattccg cccgaaaggc
     1441 agcttcgagc caatcaccga gatgatcggc ggcgggccgt tccgattgga cccgggccag
     1501 tggaccgacg acacgtcgat ggcgttgtgt cttgcggaga gcctgctgga aaagggc
//
LOCUS       DATIMH010000638         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2752864, whole genome shotgun sequence.
ACCESSION   DATIMH010000638 DATIMH010000000
VERSION     DATIMH010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2752864"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..913
                     /locus_tag="VLI90_11010"
     CDS             53..913
                     /locus_tag="VLI90_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14781.1"
                     /translation="MTLNSGDTESAVFTGTIDSVRRWESGIEITALDAGGVMARFRPA
                     TTYEHVTASSVVQSLASDAGADCGSLDNGADLSYYVADPARTAWEHVARLAAWSGALA
                     TVSADNKIELKVVQTTAADFALRYGREILAIEHTKRAAPVASFTVAGESGTGSASSPD
                     ARKAVTDFFAGNRPDGPSATAIWEWEPALRTSSAAATAGSARLRAYGSARDAGKLTAI
                     LLPQARPGAVMEMQDATGGLPAGPVWIRRVRHSVTGGRALTTVWFAGSGDASGASLLG
                     SAASAIGGLL"
     gene            913..1584
                     /locus_tag="VLI90_11015"
     CDS             913..1584
                     /locus_tag="VLI90_11015"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HSV14782.1"
                     /translation="MQAVVQTIREIARHELGQRWNTCLGVVKSVQGATNYSCTVELRE
                     SHLVLPEVPIATSAMGLAGLPSEGDLVVVLFIGGDLHAPVVIGQLYSEKIAPPDNDSA
                     ILVGVRPGGETDAKKRLEWRITTKDDGKRDLHLILDGDVKVEVTVNDEGIALAAQDTT
                     FTLKQTSSSDGKAELKVGNSKVTVEQGGNVTVEAEGTLTLKANKIEITADAQVKIAGQ
                     AVDIN"
     gene            1592..>1818
                     /locus_tag="VLI90_11020"
     CDS             1592..>1818
                     /locus_tag="VLI90_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HSV14783.1"
                     /translation="MGQPAAKANDQITATDTHIVIVPAGPAQVPTPLPHPFVGIINGG
                     TVSTVKIAGQPAAVVGSTADNTPAHIPTPPGV"
BASE COUNT          358 a          632 c          573 g          255 t
ORIGIN      
        1 catactccct tccgccgcgc cattctccgc ggcagaaggc gacgccgccg aattgacgtt
       61 gaacagcggc gataccgaaa gcgccgtctt cacgggcaca atcgattccg tgcgccgctg
      121 ggaatcgggc atcgaaatca cggcgctcga cgcgggcggc gtgatggcgc gattccgccc
      181 cgccaccact tacgagcacg tgaccgccag cagcgtcgta caatcgctag cctccgacgc
      241 cggcgccgat tgcggctcgc tcgacaacgg cgccgacctc tcgtactacg tcgcggaccc
      301 cgcccgcacg gcctgggaac acgtcgcgcg gctcgcggcc tggagcggcg cgttggccac
      361 cgtatcggcc gacaacaaga tcgagttgaa agtcgtgcaa accacagcgg cggatttcgc
      421 gctgcgctac ggccgcgaaa tactcgccat cgaacacacc aaacgggccg ccccggtcgc
      481 ctccttcacg gtggcaggcg aaagcggtac cgggtccgcc tcgtcgcccg atgcgcgcaa
      541 agccgtcacc gatttcttcg ccggcaaccg ccccgacggt ccgagcgcaa ctgcaatttg
      601 ggaatgggaa ccggcattgc gcacgtcctc cgcggcggcg accgccgggt cagcgagact
      661 gcgcgcctac gggtcggcgc gtgacgcagg gaaactcaca gcgattctgt tgccccaagc
      721 gcggcccggc gcagtcatgg agatgcagga cgccaccggc ggtctgcccg cgggtccagt
      781 gtggattcgc cgcgtgcgcc actcggtcac cggcggccgc gcgcttacga cggtgtggtt
      841 cgccggcagc ggcgatgcga gcggcgcaag cctgctgggc tcggctgcat cggccatcgg
      901 aggccttctc taatgcaggc cgtcgtgcaa accatccgcg agatcgcgcg gcacgaattg
      961 gggcagcgct ggaatacctg tctcggcgtc gtcaaatccg tgcagggcgc cacaaattac
     1021 tcatgcaccg tggaactccg cgagtcgcat ctcgtgctgc ccgaagttcc catcgcaacc
     1081 agcgccatgg gcctcgcggg actgccctcg gaaggcgacc tcgtagtcgt actcttcatc
     1141 ggcggcgatt tgcacgcgcc cgtcgtcatc gggcaactct acagcgaaaa aatcgcgccg
     1201 cccgacaacg attccgcgat cctcgtcggc gtgcgtccgg gcggcgagac cgacgccaaa
     1261 aaacgcctcg aatggcgcat cacgaccaag gacgacggca agcgcgacct ccacctgatc
     1321 ctcgatggcg acgtgaaggt cgaagtcaca gtgaacgacg aaggcatcgc gctcgccgcg
     1381 caggacacca cgttcacgct caagcagacc agcagttcgg acggcaaggc ggagctcaaa
     1441 gtgggcaaca gcaaggtcac cgtggagcaa ggcggcaacg tgaccgtgga agcggaaggc
     1501 acgctgaccc tcaaggcgaa caaaatcgag attacggccg atgcccaggt gaaaatcgcc
     1561 gggcaggcag tggacatcaa ctaggagaat tatggggcag cccgcagcca aagccaacga
     1621 ccagatcacc gccaccgaca cgcacattgt gatcgtgccc gcgggtccgg cgcaggtgcc
     1681 cacgccgctg ccgcatccct tcgtgggcat catcaacggc ggcacggtga gcaccgtgaa
     1741 gattgcggga caaccggcgg cggtcgtcgg ctccactgcc gacaacacgc ccgcgcacat
     1801 ccccacgccg ccgggagt
//
LOCUS       DATIMH010000639         5165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2464003, whole genome shotgun sequence.
ACCESSION   DATIMH010000639 DATIMH010000000
VERSION     DATIMH010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464003"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(677..1804)
                     /locus_tag="VLI90_11025"
     CDS             complement(677..1804)
                     /locus_tag="VLI90_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HSV14784.1"
                     /translation="MRQAVDNTLEPLESRMMFSVTAVAAGGVLIVNGDTNANVITVSR
                     DVTGNLLVNSGAVNITGTTPATVASIHDLQIFGQDGNDSITLDTTNGALPAAKVSADS
                     GNDTVIGGAGDDIIDGGAGNDMLLGTDGADTLHGGAGDDLIDGNGGADTAFLGAGNDT
                     FVWDPGDGSDIVEGGTDTDAMVFNGSAASENIDLSNNAGRLKFFRDAGRITMDVNDVE
                     TVNFNALGGADNVTIHDLRGTDVTKVNVNLAGPNRAADDQLDSVVVEGTNARDLITVG
                     GGGLGGISVGGLAARVNVTGADPTDKLAVNGLDGDDLIIASALNANSIAFSADGGRGN
                     DILIGSAGNDTLLGADGNDILIGAAGTDQLNGGSGLNVVIQ"
     gene            2204..2920
                     /locus_tag="VLI90_11030"
     CDS             2204..2920
                     /locus_tag="VLI90_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HSV14785.1"
                     /translation="MKTIGMLGGFGPQATMDFEARVHRVAEGLVSAHRNEGYPPMVSW
                     YCRHPPFLLGADGLPQLPRRLEAGLAEAATRLGALVDFVCIPSNGVHMLAGEIEQAAG
                     RPVLSIIETTLAEVRRRGWKRVGVLGLREPVIYTRPLHRLSIDCQILDASSRRALDHA
                     IFRTMEGRGSDAPYTAAARVAVAKLRGRNIDGIILGCTELPILLGQGGGQRRRRPDQP
                     DATARRASGELCDQRQSCPD"
     gene            3050..4648
                     /locus_tag="VLI90_11035"
     CDS             3050..4648
                     /locus_tag="VLI90_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSV14786.1"
                     /translation="MTTRADQEIPILTEIGTILSATLELREAFGKIMQIISDKLNMHR
                     GALVLLDESTGRLRTEAAAGMTPEEIERGKYALGEGVTGNVVATGRARIVPDVRAEPD
                     FLNRTGRLNPEAEGAMSFLCVPIRIEGRTAGALSVDKPFESEDQLQGDHAFLTIIAAF
                     LAQAIQINRMVMRQKEELLEENAQLRAQVRDRYRFENIIGDSPAMHEVFATVGQVANS
                     RATVLLLGETGTGKEMIAKAIHYNSPRKEKPFVRVNCGALTGTLLESELFGHVRGSFT
                     GAIRDKMGRFEAADGGTIFLDEIGTLEPQLQVKLLRVLQEREFERVGDTQTVKVDVRV
                     IAATNVDLQEEVAKGSFREDLFYRLNVVSIYLPPLRNRREDIPRLIDYFLDKYNGMND
                     RRLRRISRDMLNVLLRYPWPGNVRELENAIERAVVLSNDEDFTEELLPLSVRMFAAQR
                     RTNQASESIESLTRRLADQAIVDYEMREGEIYQLVIDQIEHALIDRALNKCGGVKTKA
                     ADFLGINRNTLNKKVKELGIQTAD"
     gene            4745..4969
                     /locus_tag="VLI90_11040"
     CDS             4745..4969
                     /locus_tag="VLI90_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14787.1"
                     /translation="MNGEIRMTKRRDARVFGHSDFAIRISLLKITLTPALSRHTGRGG
                     KTKSLPKMDSFAPVAVHFHSITVPDRIDHA"
BASE COUNT         1003 a         1518 c         1666 g          978 t
ORIGIN      
        1 ccgctgagcg cgtgtgcgcc ccatgtggcg gcgacgacga cgaccacggc tgccaacgac
       61 gccaccgcca cgcacgcgat ggccaagcag accagctgaa cgtgcaggga tcgacgagcc
      121 agaaactgtc gcacgcgggc agcctttcaa cgctcgatgc cagcggcgcc aacggcgcaa
      181 caagtcgcgt tgcggcggca tctcaaacgc gtcagttcaa atatcgacgg aattccaaag
      241 atgctggaat gggccgcggg gggttctaag tcgaaagtta agacgagcgg cggcggttga
      301 cgaggcgacg ggggaagtct gtcatgcgcc actcgcgtaa ggcgtgaaca gaacagtcct
      361 taagaggcga gccctgtcat cctgagcaag gcggtactcc gccggagtcg aaggatcttg
      421 ccgaggagcc gacgaaccgt aactcgcgcg caccgcacgg cagtggcttg cggtttcgca
      481 gacggacaag ccaccgttac tggtctgcca aaccgcaggc gaccaccgcg ccgtttgcgc
      541 gagcgatatt tcacttactc ctcggcaaga tccttcgact ccggcgagta cgcctgcgct
      601 ggaggatgac gagaaaagag accaaggtgc caaacaggcc gccctggtct tatagatgcg
      661 tgcaccgaaa ctcacatcac tgaatcacaa cgtttaaccc gctgccgccg ttgagttgat
      721 cggtgccggc ggccccgatc aggatgtcgt tgccatcggc gcccagcagc gtgtcgttgc
      781 ccgcgctgcc gatgaggatg tcgttgccgc ggccgccatc ggcgctgaac gcgatactgt
      841 tggcgttgag cgctgatgcg atgatcaggt catcgccatc gaggccgttg accgccagct
      901 tgtcggtcgg atcggcgccg gtgacgttga ctcgggccgc cagcccgccg actgatatac
      961 cgcccaatcc gccgccgccg accgtgatca gatcgcgggc attggtccct tcgacgacga
     1021 cgctgtcgag ttgatcgtcg gcggcgcgat tcgggccggc gaggttcacg ttcaccttcg
     1081 tgacatccgt gccgcgcagg tcatggatcg tgacgttgtc ggcgccgccc agggcgttga
     1141 agttgacggt ttccacgtcg ttcacgtcca tcgtgatgcg gccggcatcg cggaagaact
     1201 tgagccggcc ggcgttgttg gacagatcga tgttttcgct ggcggcggag ccgttgaaaa
     1261 ccatcgcatc ggtgtcggtg ccgccttcga cgatgtcgga gccgtcgccc ggatcccaaa
     1321 cgaaggtgtc attgcccgcg ccgaggaagg cggtatccgc gccgccgttg ccgtcgatca
     1381 aatcgtcacc ggcgccgcca tgcaatgtgt cggcgccgtc ggtgccgagc agcatgtcgt
     1441 taccggcacc gccgtcgatg atgtcgtcac cggcgccacc aatgacggtg tcgttgccgg
     1501 agtccgccga caccttggcc gcgggcaggg cgccgttggt ggtgtcgagc gtgatgctgt
     1561 cgtttccgtc ctgcccgaag atttgcaggt catggatgct ggcgactgtt gcgggcgtcg
     1621 tgcccgtgat gttcacagcg ccgctattca cgagcaggtt accagtgacg tcgcgcgaga
     1681 cggtgatgac gttggcgttg gtgtcgccgt tcacgatgag gactcccccg gcggcgacgg
     1741 ccgtgacgga gaacatcatc cgcgattcga gcggttcgag cgtgttgtca acggcttggc
     1801 gcaacgcagt cgactcaaag gggcgccggc gcgaccgccg cgacgacgag aacagggtcg
     1861 agaatcgatt ggcaaaagtc atggtcatgt ccttcctccg acaggagggg ttggaattcc
     1921 gctcggtagg agtcattccg gccgggcatg ttggatctgg cgatttcctt ggcgagcgtt
     1981 tggcgactcg cgtttcaaga agaacgttta ttcccttcat agtaaaagca atcatttcgc
     2041 ggccagctct tgctggttga ccgctgtacg tgggcatata cgcggtcagc ggcatcaatt
     2101 tcgaaatcgt ttcatgtccc gattcaggta catgccgcac ggctcgagcg gttcctaatc
     2161 tccaccaggc gcgcgggact atttgagatt acggatgaac agcatgaaaa cgatcggtat
     2221 gctcggtggg tttggtccgc aggcgacgat ggacttcgag gcgcgcgttc accgcgtggc
     2281 cgaaggcctc gtgtctgccc atcgcaacga aggctatccg ccgatggtct cgtggtactg
     2341 ccggcatcca ccgtttctgc tgggagcgga cggtttgccg caactgccgc ggcggctcga
     2401 agcgggactg gcggaagcgg ccacgcggct gggcgcgctg gtggacttcg tttgcatccc
     2461 atctaacggc gtgcacatgc tggccggcga aatcgaacaa gcggcggggc gaccagtcct
     2521 gagcatcatc gagacgacgc tggccgaggt gcggcgtcgt ggatggaagc gcgtcggggt
     2581 gctggggctg cgcgagccgg tcatctacac gcggccgctg catcgtttga gcatcgactg
     2641 ccagatcctc gatgcctcgt cgcggcgggc gctggaccat gcgatcttcc gaacgatgga
     2701 aggtcgcggc agtgacgcgc cttatactgc cgccgcgcgc gtggccgtcg ccaaactgcg
     2761 cgggcggaac atcgacggca tcatcctcgg ctgcaccgag ctgcccatcc tgctcggcca
     2821 gggtggcgga caacgacgac gacgacctga tcaacccgac gcaactgctc gccgagcgag
     2881 cggtgagctt tgcgatcagc gacagtcctg tcccgattga agcggtgcaa tgagcttaga
     2941 ttaagcaccg acgcctcggc cccgtgcgtg caactttatt tgacattcat cgcgtattac
     3001 tgcatatttt gtcgtcacta tctctcggtg tacgcatacg gagcaggcaa tgaccacgcg
     3061 ggccgaccaa gaaatcccca ttctgactga gatcggtact atcctaagtg ccacgctaga
     3121 gttgcgcgag gcgttcggga agatcatgca gatcatttcc gataaactga acatgcacag
     3181 gggggcgttg gtgctgttgg atgagtctac cgggcggttg cgcaccgaag cggcggcggg
     3241 gatgacgccg gaggagatcg agcgcggtaa atacgcattg ggtgaaggcg tcacgggcaa
     3301 tgtggtggcg acggggcggg ctcggatcgt gccggatgtg cgtgctgagc cggatttcct
     3361 taaccgcacc gggcggttga atccagaggc cgagggggcg atgagcttcc tatgcgtgcc
     3421 catccgcatc gaaggccgaa cggccggcgc gttgtcggtt gataagccgt tcgagtcgga
     3481 agatcagctg cagggcgacc acgcgttcct gaccatcatc gcggcgttcc tcgcgcaggc
     3541 gatacagatc aaccgcatgg tgatgcgcca gaaggaggag ctgctggaag aaaatgccca
     3601 gctgcgggcg caggtgcgcg atcggtatcg gttcgagaac atcatcggcg actcgccggc
     3661 catgcacgaa gtcttcgcca ccgtcggcca ggtcgccaac agccgcgcca cggtgctgct
     3721 gctgggcgag actggcacgg gcaaggaaat gatcgcgaag gcgattcatt acaacagtcc
     3781 ccgaaaagaa aagccgttcg tgcgcgtgaa ctgcggcgcg ctgaccggca cgttgctgga
     3841 atcggaattg ttcggccacg tgcgcgggtc gttcaccggc gccattcgcg acaagatggg
     3901 tcgattcgaa gcggccgacg gtgggacgat cttcctagat gaaatcggca cgctggaacc
     3961 acagcttcag gtgaagctgc tgcgcgtgtt gcaagagcgc gagttcgagc gcgtcggtga
     4021 tacgcagacg gtgaaggtgg acgtgcgcgt gatcgccgcc accaacgtcg atttacagga
     4081 agaagtcgcc aagggcagct tccgcgaaga cctgttttat cgcctgaacg tcgtcagcat
     4141 ttatctgccc ccgctgcgca atcggcggga agatatcccg cggttgatcg attacttcct
     4201 cgacaagtac aacggcatga acgaccgccg gctgcggcgg atcagccggg acatgctcaa
     4261 cgtgctgcta cggtatccct ggccggggaa cgtgcgcgag ttggaaaacg cgatcgagcg
     4321 cgcggtcgtt ctcagcaatg acgaagactt caccgaagag ctgctgccgc tgagcgtgcg
     4381 gatgttcgcg gcacagcggc ggacgaacca ggccagcgaa tcgatcgaga gcctcacccg
     4441 ccgactggcc gaccaggcga tcgtcgatta cgaaatgcgc gaaggcgaga tttatcagct
     4501 ggtgatcgac cagatcgagc acgcgttgat cgaccgcgcc ctgaacaagt gcggcggcgt
     4561 gaagaccaag gcggccgact tcctcgggat caaccgcaat acgctgaaca agaaggtgaa
     4621 ggagttgggc atccagacgg cggattgagc gcttccggtc ccttcgcccg gtactccggg
     4681 agagggttag ggtgagggtg atttccgaat gacgaatgac gaatgacgaa attcgaatcc
     4741 ccgaatgaat ggcgaaatcc gaatgacgaa acggcgcgat gcccgcgtat ttggtcattc
     4801 ggatttcgcc attcggattt cattactcaa gatcaccctc accccagccc tctcccggca
     4861 taccgggcga gggggcaaga cgaaaagtct tccaaaaatg gatagtttcg ctcccgtagc
     4921 agtccacttc cactccatta ccgtgcccga tcgcatcgat cacgcctgat ccgcccgcga
     4981 ttattggcgg tttcacttcc gatattcagc aatctcccac gctgtaaaaa tccgcaagac
     5041 gccgcgctgc cgcgccgaca taccacccgt gtccgtgcgt cgaggtgttc gatgcggaaa
     5101 ggtttgtatg tgccgcggcc gctccatttg tttcgccttc tatgttcttg cagttgttgc
     5161 gctgg
//
LOCUS       DATIMH010000640         5349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1022862, whole genome shotgun sequence.
ACCESSION   DATIMH010000640 DATIMH010000000
VERSION     DATIMH010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022862"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VLI90_11045"
     CDS             <1..257
                     /locus_tag="VLI90_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV14788.1"
                     /translation="TAEKFRPHVILLDMHLSDIDGREVAKQVKGNPDLQLTKVIAVSG
                     KMSDEEAKGLLAQGFDGFLRKPFHVRQVIEAVEDAVAVVY"
     gene            complement(413..1198)
                     /locus_tag="VLI90_11050"
     CDS             complement(413..1198)
                     /locus_tag="VLI90_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14789.1"
                     /translation="MKAWKLAVAVIGLSPLFGRAEVPAPPTADEVAALNPPPAPPAPD
                     PRTASPAPAPGSDGNGGGGFRGGFGGYGGRWGPWGRLLPGDVYSPQDVEQATPWFKDH
                     SPKRWAVVSALPDEGRRKMRLTGALMRVYRNVQTVQKDDPDLAQLITNRVELEDAAFD
                     LVSQYRAAKKAGDTSKQAALQHDREQKVSDLVDVNIKERRARIARLEKTLAQEQTKLA
                     DDEKSKDQLVEKRLRSLIDGQPAPDGAAGTDSNQPDQVGSVQR"
     gene            complement(1243..1836)
                     /locus_tag="VLI90_11055"
     CDS             complement(1243..1836)
                     /locus_tag="VLI90_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14790.1"
                     /translation="MPSLLQQLQNNEAVLQMYLADELPADDRIEVEQMLATDPGLRAV
                     LEDLRLAQDLVIGSLRKLDQATPLAVSTPVAARQMSRLIKQWQVDRLERQAAAPIKQK
                     FRLPAWAYSAATAAAVLVAVVVWWGMKSDVPGEPHRDEPQQYTFEDDPSFQVVRSLVN
                     QDDAASGIAEAEQEASALARSDDSSSILSFESSDNVN"
     gene            complement(1903..2493)
                     /locus_tag="VLI90_11060"
     CDS             complement(1903..2493)
                     /locus_tag="VLI90_11060"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV14791.1"
                     /translation="MSAQVASQAGSAGPSPGETDEALALRLRDGDTAAGEVLVKRFHQ
                     PLFRYLHRLAGTDHLAEELHQQTWLSVLDHIEKFDPASTTGGFKAWLFRIATNKANDY
                     WRSRSREKSATEGLKLVTEEALPAAGERLENTEQQLKLQRAIQQLPEAQRQVLMLRYY
                     SNLRFVEIADMLGCPLNTALGRMHKAVLKLRQLMEA"
     gene            2699..4213
                     /locus_tag="VLI90_11065"
     CDS             2699..4213
                     /locus_tag="VLI90_11065"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HSV14792.1"
                     /translation="MSSVDLENTLRDLPRLGTLVKDRGYRQVWRFVHNDRAYYLKFYP
                     KGGPRDRFRRFFRGSPALREFSRLQALQKANIPAPRAVAALMGFAINDRRGDAVVLEA
                     IEPSIQLDQLLSNLELRGEPIPDHLYLASQIRALVQQLAKGKLGHEDLHLGNLLLHQN
                     NLYLLDGYAVRSGGLQLRDLLMLGHSVRRYATLTDLQRGWDTLGPGGRMPLRNPHSDF
                     LWKRFLQGITKENRYFGRLNIDGWTGVYYKHTKYPHRWSAASKLEVTAADWQREWPLL
                     LQRIESDQLEVIKRSRSGEVFAGEIVLNGRPLQVIVKRPRRRYWYRYINEIGRGSRPR
                     RAWRKAWNLLVRGLPTAWPLLVMERRSLGYVTDAIFICERVPGATLAHADLDALPDEK
                     RDRLFHRTGKILREIERLGFSHFDAKASNWIVFEDDRLGPTPVLIDIDGIRRRRWIAL
                     GIRRLLKSMHENPHYAPIDSLALCRGYAPYAAMDASSVIAAGDSQREPSQAKAV"
     gene            4269..5327
                     /gene="waaF"
                     /locus_tag="VLI90_11070"
     CDS             4269..5327
                     /gene="waaF"
                     /locus_tag="VLI90_11070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HSV14793.1"
                     /translation="MRATSPPIFEKPPQRVLIIKPSAIGDVVHALPVLNLLRRRWPDA
                     HISWLVTPACAGLLDGHPQINEVILFDRKRYGRSWRSPSALRDLSSFNRSLKSQKFDL
                     VIDLQGLFRSGWLTWRTHAPVRVGSTSAREFSWVFCTHLAQADMNGQHAIDRYLNVAE
                     FLGLGRGPVEFVFPTDDRDRQYIKTLLPPGEKFAVLLPATNWPTKQWPIERFAAMTEP
                     LREKYGFTSVLAGGPDAVPLAASLPGAINLAGKTNLRQLVALLERASLVIANDTGPMH
                     IAAALGRPLVTMFGPTSPVRTGPYQQMDSVVRLDIPCSPCFSRRCSHQSCLRWLEADP
                     VLRLAEEQLHVSSSTHIV"
BASE COUNT          924 a         1784 c         1663 g          978 t
ORIGIN      
        1 tcacggcgga aaagttccgc ccgcacgtca tcctgctgga catgcacctg agcgacatcg
       61 acggccgcga ggtggccaag caggtgaagg gcaacccgga tcttcagctg accaaggtga
      121 tcgcggtcag cgggaagatg agcgatgagg aggcgaaggg cctgctcgcg cagggcttcg
      181 acgggttcct ccgcaagccg ttccatgtgc ggcaggtgat cgaagcggtt gaggacgccg
      241 tggccgttgt gtactgattt ttgctttagg gaaactgcaa caagagcgaa cccggcgata
      301 gtcgcggcgt agctttgtta gcagtcacca tatttgtgcg ttcgaaagaa cgcaggggcc
      361 gctcccggag gggacggcct tttttcttgc gccgattttc gaagaggaaa tgctatcgct
      421 gaacggaccc gacctggtcc ggttggttcg aatccgttcc cgcggcgcca tcgggcgcgg
      481 gttggccatc gatcaagctg cgcagccgct tctcgacgag ttggtcctta ctcttctcgt
      541 cgtccgccag cttcgtttgc tcctgcgcca gcgtcttctc cagacgcgcg atgcgcgccc
      601 ggcgctcctt gatgttcacg tcaaccaggt ccgagacctt ctgctcccga tcgtgctgca
      661 gcgcggcttg cttcgaagta tcgccggcct tcttggcggc gcggtattgg ctgaccagat
      721 cgaacgcggc gtcttccagc tcgacgcgat tggtgatcag ctgcgccaaa tccggatcgt
      781 ccttctgcac ggtctgcacg ttgcggtaga cccgcatcag cgcgccggtg aggcgcatct
      841 tgcgccgccc ttcatccggc agcgcgctga cgaccgccca gcgcttgggg gaatggtctt
      901 tgaaccacgg cgtggcctgt tcgacatcct gcgggctgta cacgtcgccc ggcaacagcc
      961 ggccccacgg cccccaccga ccgccgtacc cgccaaatcc accgcggaaa ccacccccac
     1021 cgttgccgtc actgcctggc gcgggcgcgg ggctggccgt gcgcggatcg ggcgcgggtg
     1081 gcgcgggcgg gggattcagc gccgccacct catccgccgt cgggggcgcc ggcacttcgg
     1141 cgcgcccaaa cagcgggctc agaccgatca ccgcgactgc cagtttccac gctttcatgt
     1201 tcgatcgcct ccccgtcatt cccgtcgaga agcaccgcat ctctagttca cgttatcact
     1261 cgactcgaac gacaggatag aggaactatc atccgatcgc gccaatgccg atgcttcctg
     1321 ctccgcctcg gcaataccgg aggcggcgtc gtcctggttc accaatgagc ggaccacctg
     1381 gaagcttgga tcgtcttcga atgtgtactg ctgcggctcg tcacgatggg gttcgcccgg
     1441 cacatcggac ttcatgcccc accacacgac gaccgccacc agcaccgccg ccgcggtcgc
     1501 cgcggaatac gcccacgccg gcaagcggaa cttttgcttg atcggcgccg ccgcctggcg
     1561 ctccaggcga tcgacctgcc actgcttgat caagcgcgac atctgccggg ccgccacggg
     1621 tgtggagacg gcaagcggcg tggcctgatc cagcttgcgc agcgagccaa tcaccaagtc
     1681 ctgcgctaag cgcaggtctt ccaacaccgc gcgaaggccc gggtcggtgg cgagcatctg
     1741 ctcgacctcg atgcggtcat ccgccggaag ttcgtccgcc agatacatct gcaggactgc
     1801 ttcgttgttc tggagttgtt gcagcaaact aggcatcgtt tcgctcttgt agctgcgacg
     1861 cctgcgtcgc cctttcgcca cgaggcgacg caggcgtcgc agctacgctt ccatcaactg
     1921 tctcaacttc agcaccgcct tgtgcattcg gcccagcgcc gtgttcagcg gacatcccaa
     1981 catatcggcg atctcgacga acctcagatt gctgtaatac cgcagcatca acacttgccg
     2041 ctgcgcttcg ggcaactgct ggatcgcccg ctgcaacttc agctgctgct cggtgttctc
     2101 cagccgttca ccggcggcgg ggagcgcttc ttccgtgacc agcttcaacc cttccgtcgc
     2161 cgacttctcc cgactgcgcg accgccaata gtcgttggcc ttgttagtcg cgatgcggaa
     2221 gagccaggct ttgaaacccc cggtggtcga tgccgggtcg aacttttcaa tgtggtcgag
     2281 aacgctcagc caggtttgct gatgcaattc ctcggccagg tggtcggtcc ccgccaggcg
     2341 gtgcaggtat cgaaacagcg gttggtggaa ccgcttgacc agcacctcac cggcggccgt
     2401 gtccccgtca cgcaatcgca gcgccagggc ttcgtcggtt tcgcccggcg acggaccggc
     2461 cgaaccggcc tgtgaagcaa cttgcgcgga catcggattg ataccggccg tcatggtttg
     2521 taacgcacgg ccgggccgat tattgcaagc ggtctgcaag tggacaccgg cgaccttggg
     2581 tgcgggcacg agccctcata gccgcttttg ccggcgtatc ttacacacgc ccgcggccgg
     2641 ggggcaaatt gattctgtgg caagacaaac ccgccggcca cgcatataac gttaccgcgt
     2701 gtccagcgtc gatttggaaa atacgcttcg cgatctgccg cgcctgggca cgctcgtcaa
     2761 agaccgcggg tatcgccaag tctggcggtt cgttcacaac gaccgggcat actacctgaa
     2821 gttctatccc aagggcgggc cgcgcgaccg cttccgccgg ttcttccgcg ggagcccggc
     2881 actgcgcgag ttcagccggc tgcaggcgct gcagaaggcc aacatcccgg ccccgcgcgc
     2941 ggtggccgcg ttgatgggtt tcgcgatcaa cgaccggcgc ggcgacgcgg tggtgctgga
     3001 ggcgatcgag ccgtcaatcc agctggatca actattgagc aacctggagc tgcgcggcga
     3061 gccgattccg gatcacctgt atcttgcatc gcaaattcgc gcgctggtgc agcagttggc
     3121 gaaggggaag ctgggccacg aggacctgca cctgggcaac ctgctgctgc atcagaacaa
     3181 cctttacttg ctcgatggtt acgcggtgcg cagcggcggt ctgcagcttc gcgatctgct
     3241 catgctcggc cacagcgtcc gccgctatgc gacgttgacc gaccttcagc gcggctggga
     3301 cacgctcggc cccggcgggc ggatgccgct gcgcaacccg cacagcgatt ttctttggaa
     3361 gcgattcctg caaggcatca cgaaagagaa tcgctacttc ggccggttga acatcgatgg
     3421 ttggaccggc gtttattaca agcacaccaa gtacccccat cgctggtcgg ccgcgagcaa
     3481 gctggaagtg accgccgccg attggcagcg cgagtggccg ctgctgttgc agcgcatcga
     3541 gtcggaccaa ctcgaggtga tcaagcgcag ccgcagcggc gaagtcttcg ccggcgagat
     3601 cgtattgaac ggccggccac tgcaagtgat cgtcaaacgc ccgcggcgac gatactggta
     3661 tcgctacatc aacgagatcg gccgcggctc acgcccgcgg cgggcgtggc gaaaggcatg
     3721 gaacctgctc gtccgcgggc tgccgacggc gtggccgctg ctcgtgatgg aacggcgatc
     3781 gctcggctac gtcaccgatg cgatcttcat ctgcgagcgc gtgcccggcg cgacgttggc
     3841 ccacgccgat cttgatgcgc tgccggatga aaagcgtgac cggctgttcc accgcacggg
     3901 gaaaatcctg cgcgagatcg agcggctcgg cttctcgcac ttcgacgcca aggcgagcaa
     3961 ctggatcgtg ttcgaggacg atcgcctcgg cccgacgccg gtgctgatcg acatcgacgg
     4021 cattcgccgg cggcggtgga tcgcgctggg aattcgcaga ctgctcaaga gcatgcacga
     4081 gaacccgcac tacgcgccga tagattcgct ggcactatgc cgcgggtatg cgccgtatgc
     4141 agcgatggac gcgtcatccg tgatagccgc aggcgacagc cagcgggagc cttcgcaggc
     4201 gaaagcggtt tgacagtcga acgatcccgc tggctgtcgc ctgcggctgt gaaagatttg
     4261 caccactgat gcgcgccacg tcgccaccga tctttgagaa acccccgcag cgcgtgctga
     4321 tcatcaagcc cagcgccatc ggcgacgtcg tgcacgcgct gccggtgctg aacctgctgc
     4381 gccggcgctg gccggacgcg cacatcagct ggctcgtcac ccccgcgtgc gcgggcttgc
     4441 tcgacggtca tccgcagatc aacgaagtga tcctcttcga ccgcaagcgc tacggccgca
     4501 gttggcgaag cccatccgcg ctgcgcgatc tctcctcgtt caaccgctcg ctcaaatcac
     4561 agaagttcga cctggttatc gacctgcaag gcctcttccg cagcggctgg ctgacctggc
     4621 gcacgcacgc gccggtacgc gtcggttcca ccagcgcccg cgagttcagc tgggtcttct
     4681 gcacgcacct ggcgcaggcg gacatgaacg gccaacacgc gatcgatcgc tatttaaacg
     4741 tcgccgagtt cctcggcctc ggccgcgggc cggtcgagtt cgtcttccca acggacgatc
     4801 gtgatcgaca gtacatcaag acccttctgc cgccgggcga gaagttcgcc gtgctcttgc
     4861 ccgcgaccaa ttggccgaca aaacaatggc ccatcgaacg cttcgccgcg atgaccgaac
     4921 cactgcgcga gaaatacggg ttcacgtcag tcttggccgg cggccccgac gcggtgccgc
     4981 tggcggcgtc gctgcccggc gcgatcaatc tggcaggaaa gacgaacctg cggcagctgg
     5041 tcgcgctgct ggagcgggcg tcgctggtca tcgccaacga cacgggcccg atgcacatcg
     5101 ccgccgcgct cggccggccg ctggtcacga tgttcggacc taccagtccg gtgagaacgg
     5161 ggccctatca gcaaatggac agcgtcgtgc gcctggatat cccctgcagc ccctgcttct
     5221 cgagaaggtg ctcgcaccag agctgcctgc gatggctcga ggccgatccc gtgctgcgcc
     5281 tggcggaaga acaactgcac gtctcctcaa gcacgcatat cgtttagccg ccgcggccgc
     5341 gcggcgcgg
//
LOCUS       DATIMH010000641         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_735384, whole genome shotgun sequence.
ACCESSION   DATIMH010000641 DATIMH010000000
VERSION     DATIMH010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735384"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VLI90_11075"
     CDS             complement(<1..380)
                     /locus_tag="VLI90_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSV14794.1"
                     /translation="MGHLDPRFMLSGFFIGLLVGQTGMGGGSLMTPLLVLFFGIHPAT
                     AVGTDLLYASATKTAGTLVHGLNKTVDWRIVSRLAAGSVPATLVTLFAISHYNISGPT
                     SGRIIALVLGVMLLLTALSLIFRRG"
     gene            complement(432..1463)
                     /locus_tag="VLI90_11080"
     CDS             complement(432..1463)
                     /locus_tag="VLI90_11080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HSV14795.1"
                     /translation="MSKLTPGSVDLVFADPPYNLQLKGDLKRPDESHVDAVNDDWDKF
                     ESFSAYDDFTRAWLLACRRLMKPSATLWVIGSYHNIFRVGAIMQDLGFWVLNDIVWRK
                     SNPMPNFRGRRFTNAHETMIWAARSRESRHKFNYQAMKALNDDIQMRSDWLIPLCTGP
                     ERLRNQHGLKLHPTQKPEALLHRVLLASTGHDDIVLDPFAGTGTTPVVARRLGRHFIA
                     IERHPAYVEAAWGRLRRTRRGSDDGIAQTPTKRDAPRIPFGNLVEQGLIPPGTRLFDR
                     QRRVSAVVGADGTIAAGDIRGSIHKVGATLQNAPSCNGWTFWHVEREGALVPIDTLRA
                     TALSAALVT"
BASE COUNT          373 a          642 c          716 g          359 t
ORIGIN      
        1 ccgcggcgaa aaatcagcga cagcgcggtc agcagcagca tcacgccgag caccaacgcg
       61 atgatgcgtc cactggtggg gcccgaaatg ttgtaatgcg agatggcgaa cagggtgacg
      121 agcgtcgccg gcacgctgcc ggccgcgagt cgcgacacga tccgccaatc cacggtcttg
      181 ttcaggccgt gcacaagtgt gccggccgtc ttcgtggccg aggcgtagag caggtcggtg
      241 cccaccgccg tcgccggatg aatgccgaag aaaagcacca gcagcggcgt catcagggag
      301 ccgccgccca tgccggtctg cccgaccagc aggccgatga agaaacccga cagcatgaaa
      361 cgcggatcga ggtggcccat gcggatcgcc cgttcaaccg ttcacgcccg gaggttagcg
      421 gcccacccgg atcaggtcac cagcgcggcg gacagcgcgg tcgcgcgtag agtgtcgatc
      481 ggcaccagcg cgccctcccg ctccacatgc cagaaggtcc aaccgttgca ggacggcgcg
      541 ttttgcagcg tggcgccgac cttgtggatc gacccgcgga tatccccggc cgcgatcgtg
      601 ccgtcggcgc cgaccacggc ggacacccgc cgctggcggt cgaacaatcg cgtgcccggc
      661 gggatcaggc cctgctccac cagattgccg aacggaatgc gcggcgcgtc gcgcttcgtc
      721 ggtgtctgcg cgatgccgtc gtcggagcct cggcgggtgc ggcgcagccg gccccaggcg
      781 gcctcgacat aggcggggtg acgctcgatc gcgatgaaat ggcggccaag ccggcgcgcc
      841 acgaccggcg ttgtgccggt gcctgcgaag gggtcgagca cgatgtcgtc atggccggtg
      901 ctggcgagca gaacccgatg cagcagcgcc tcgggcttct gggtcgggtg cagtttcagg
      961 ccgtgctggt tgcgcaggcg ctcggggccg gtgcacagcg ggatcagcca gtcgctgcgc
     1021 atctggatat cgtcgttcag cgccttcatc gcctggtagt tgaatttgtg ccgcgactcg
     1081 cggcttcggg cagcccagat catcgtttcg tgcgcgttgg tgaagcggcg gccgcggaaa
     1141 ttcggcatcg ggttggactt gcgccagacg atgtcgttga gcacccagaa cccgaggtcc
     1201 tgcatgatcg cgccgacgcg gaagatgttg tgataggagc cgatcaccca cagggtcgca
     1261 gacggcttca tcagccggcg gcaggccaag agccaagcgc gggtgaaatc gtcataagcg
     1321 gagaacgatt cgaacttgtc ccagtcgtcg ttgacggcat cgacatggga ttcgtcgggg
     1381 cgcttgagat cgcccttgag ctggagattg tagggcggat cggcgaacac gaggtcgacc
     1441 gaaccgggtg tcagcttcga catctcggcg acgcaatcgc cgacgatgat gctgtgcgaa
     1501 gcggactcga aatttgtgcg gggcgccttt gcagacgccc cgcgacgcga cactaccatg
     1561 actcaagaac tctgactcag gcgacgctgt cggcgacgcg ggaccaaaca accgactgac
     1621 cgttacattg agggcgcaaa gtaaaaatcg acttaacccg ctgctttcgc gggcagacgc
     1681 cgcatcgggt gtcgcggcgg ttcgttcgcg cgcgttcgct gtgttttacg gtgtattttg
     1741 cctttcctcg cgtgccggtg cacctgaggc tgctatttca tctcggaaaa aattgcacgg
     1801 cggcgggaaa aaattgctgg ccccggcgcg atgccgcact aggcaatttt tgccggtcgc
     1861 gatattgatc gttgcaacgg aaattttacc tgtgtggcgc gttgagcttt cgcgcttctg
     1921 cgatcaggtg ccatcaggac gtaagggaca gccgccatgc gttacgacga tttccgccgc
     1981 agcgacgaca tcgaggaccg tcgcgacgac ggtggcgggg gcggcggcgg gttcggcctg
     2041 ccgatgggag gcggcgggct cggcatcggc accatcatcg tgctcgggct
//
LOCUS       DATIMH010000642         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4486750, whole genome shotgun sequence.
ACCESSION   DATIMH010000642 DATIMH010000000
VERSION     DATIMH010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4486750"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VLI90_11085"
     CDS             complement(<1..295)
                     /locus_tag="VLI90_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14796.1"
                     /translation="MILVARRQSGARNDGTNAQQCGTYSPHTPPVSNTDNAQCSLLLP
                     GSNSSNQLMLARSLTAVATAVSNIDQHCEPRPKQCVAPPPSTKETKHSDDQKRR"
     gene            398..>1520
                     /locus_tag="VLI90_11090"
     CDS             398..>1520
                     /locus_tag="VLI90_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSV14797.1"
                     /translation="MPGKNYASTRFSALGQITPANVSKLALSFTFSTATTHGYEAPPL
                     VVNGTMYLITPFPNYLYALDLTKRGAPAKWVFKPKPLAASQGVACCDTVNRGAVYDAG
                     KIFFNTLDGQTIAVDANSGQQVWRTQLGDIQRGETITMAPLVANGKVIVGNSGGEMGV
                     RGWVAALDEGSGKLAWRAFNTGPDKDVLIGPRFKPFYAMDQGKDLGVKTWPPDAWKIG
                     GGTVWGWVSYDPETKQIFYGVGNPGPWNPDQRPGANKWTTGVFARDVDTGEATWLYQT
                     TPHDLFDHDDINEIILADIPWGNGRRPVLLHPGRNGYFYVIDRRNGEVLSATPYGYVN
                     AYKGVDLKTGRIQPNHDKDPMVGRVVRDICPAAPGVKDWN"
BASE COUNT          292 a          487 c          476 g          265 t
ORIGIN      
        1 tccgccgctt ttggtcgtcg gaatgcttcg tctcctttgt tgatggcggc ggggcaacgc
       61 attgtttcgg gcgcggttca caatgctgat cgatgttaga aacggccgta gcaacggccg
      121 ttaagctgcg cgccaacatt aactgattcg agctgttgga acccggcagc agcaaagaac
      181 attgtgcgtt gtcggtgttc gaaactgggg gcgtatgcgg cgagtaagta ccgcactgct
      241 gagcgttagt gccgtcgttg cgtgcgccgg attggcggcg tgcaacaaga atcaatccac
      301 ctcttcatca ggcgcgaccg tttccggccc gcttcccagc ggcaaccatg cccttgcgcc
      361 cgccgcagcc gctccagcgg acgacggcaa ctggacgatg ccgggcaaga attacgcgtc
      421 cacgcgcttc tcggcgctag gccagatcac gccggcaaac gtctcgaagc tggcgctcag
      481 cttcaccttc tcgactgcga cgacgcacgg ctacgaagcg cccccgctgg tggtgaacgg
      541 cacgatgtac ctgatcacgc cgtttccgaa ctacctctac gcgctcgatc tcaccaagcg
      601 cggcgctccg gccaaatggg tgttcaagcc caagccgctc gccgcgtcgc aaggcgtcgc
      661 ctgctgcgac acggtcaatc gcggggcggt ctatgatgca ggtaagatct tcttcaacac
      721 gctcgacggt cagaccatcg cggttgacgc gaacagcggg cagcaggtgt ggcgcaccca
      781 gctcggcgac attcagcgcg gcgagaccat caccatggcg ccgctggtcg ccaacggaaa
      841 ggtcatcgtc ggcaattcgg gcggcgagat gggcgtccgc ggctgggtcg ccgcgctcga
      901 cgaaggcagc ggcaagctcg cctggcgcgc attcaatacc gggcccgata aggacgtcct
      961 gatcggtcct cgcttcaagc ccttctacgc gatggatcag ggcaaggacc ttggcgtcaa
     1021 aacctggccg ccggatgcgt ggaagatcgg cgggggcacc gtctggggct gggtcagcta
     1081 cgaccccgag accaagcaga tcttctacgg cgtcggtaac cccggaccgt ggaatcccga
     1141 tcagcgcccc ggcgccaaca aatggaccac gggcgtcttc gcgcgcgacg tcgataccgg
     1201 cgaggcgacc tggttatatc agacgacgcc gcacgacctg ttcgaccacg acgacatcaa
     1261 cgagatcatc ctcgccgaca tcccgtgggg caacggccgc cggccggtcc tgctgcatcc
     1321 cgggcgcaat ggctatttct atgtgatcga ccgacgcaac ggcgaagtgc tgagcgcgac
     1381 gccttatggt tatgtgaacg cgtacaaggg tgtcgacctg aagacaggaa ggatccagcc
     1441 gaaccacgac aaggacccga tggttggccg cgtggttcgc gacatctgcc ccgccgctcc
     1501 gggcgtcaag gattggaacc
//
LOCUS       DATIMH010000643         6566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1024061, whole genome shotgun sequence.
ACCESSION   DATIMH010000643 DATIMH010000000
VERSION     DATIMH010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024061"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VLI90_11095"
     CDS             <1..321
                     /locus_tag="VLI90_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV14798.1"
                     /translation="LEDAKLQVDVAGSAAEAINKLALVPGSYDAVIIDVGLPDRKGDT
                     LIHEMKAIYPHLPIILATGQNTRDLRTKFDDQSGLAFVSKPYTAQELLNALRSLGVGI
                     GQSV"
     gene            complement(388..732)
                     /locus_tag="VLI90_11100"
     CDS             complement(388..732)
                     /locus_tag="VLI90_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV14799.1"
                     /translation="MTHTILLVDDDAGVRDVIATMLEDMGCHVICASSGSEALELLQR
                     DDSISILITDIDMPSMDGHELAERATRLHPALKVLQVSGRERRRGGFPTIRKPFSEDD
                     LVRVMQQTTGAC"
     gene            complement(1072..2241)
                     /locus_tag="VLI90_11105"
     CDS             complement(1072..2241)
                     /locus_tag="VLI90_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSV14800.1"
                     /translation="MSEAIEPVKPSGLHGVARVEGASHAHLAGRTPEQACSPPASKRY
                     KLIARLARRPTAASDRLRRCLLDTRGEKHTMKILYFDDFKLGVLKGDVVVDVSAAVED
                     IPHTGPGDVMNGLIEHWSSYKARLEAAAASGDGLPLSSVRIRPPVPGPRTIDCMAVNY
                     MEDGTRSAPAPLNAFHKSPGAIIGHGDIMVLPDVPATCFEGEAEIAAVIGKKCSHVSE
                     AEAMDHVFGYMNFIDGSARGLGPPANVFFQMKSRDTFAPIGPWLVTSDEIKDPLKLQI
                     RLWNNGVLMQNFNTDDMGHKIAKCIAWLSSIHTLLPGDIVATGTNHRGLNPFMDGDSI
                     ELETEGLGRLKFGIKDDLKRTWARKTRLQMHEEAKEKTPGLYPDLTPQLSGKYAK"
     gene            complement(2238..2792)
                     /locus_tag="VLI90_11110"
     CDS             complement(2238..2792)
                     /locus_tag="VLI90_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14801.1"
                     /translation="MVFRVLFVATFFMVTFSNASNARPFRSYSAPRECNVTMPCDFTG
                     MRQPQIRTSYRMMEQRVPAQPVVNYRNAMASADAMAGVDSGRVVGGRPAGCPNSFCGC
                     GASLRVFGRIVPGLNLAANWLRFPRTAPAPGMVAARRGHVFVLEQQVAGDTWMAYDAN
                     SGGHATRIHARSLRGYTVVNPHAA"
     gene            complement(2911..4173)
                     /locus_tag="VLI90_11115"
     CDS             complement(2911..4173)
                     /locus_tag="VLI90_11115"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV14802.1"
                     /translation="MIRTFRSLANHNYRIWAAGALVSNIGTWMQRAAQDWLVLTELTH
                     QSASAVGIVMALQFGPQLLLMPWSGYAADHFNQRKLLMATQATMGALSLILGLLTTTG
                     LVQLWHVYVFAFLFGCSAALDAPVRQTFVAELVGERDLSNAIGLNSTSFNAARMIGPA
                     AAGLLIAAVGTGWAFLFNGASYMAVLVSLCLLRTSELRPNARAIRAKGSVLQGFHYVW
                     SRPDLRATLVMLFLIGTFGLNFPIFISTMAVGVFHTDARGFGLLSSMMAIGTMSGALL
                     AASRERPSFTSLLAGSATFGLGCTLAALAPNYWLFAAALVVIGVASLTVLNTSNALMQ
                     LSTEPAMRGRVMALRLGVALGGTPVGAPIVGWVADHLGPRWALGVGAASGFAAAAVAI
                     GALSQSKRSAEYRGHQAQPSEFDPPRQS"
     gene            complement(4170..4499)
                     /locus_tag="VLI90_11120"
     CDS             complement(4170..4499)
                     /locus_tag="VLI90_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14803.1"
                     /translation="MNWLELTAYFFGGAFLTNAIPHLVAGLRGEPFQSPFAKPPGQGL
                     SSSIVNLLWGFFNLVIGYLLVCRVGDFALRETADVAALGLGGLLIGLFLARWFGRFRG
                     GNEPRQT"
     gene            complement(4847..6190)
                     /locus_tag="VLI90_11125"
     CDS             complement(4847..6190)
                     /locus_tag="VLI90_11125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV14804.1"
                     /translation="MKATYNRNRGATMLDWFKELSRMERKGFYGAFLGHAVDIFDFMI
                     YSFLIPTLLTQWAMSKSAAGAIVTWTLISSLVGAIGAGLLADRYGRVRVLRWTIVVFA
                     VACFLCGMANSPEQLMIFRTIQGLGFGGESSLCMVLVTEMIRNPAHRGKYSGFTASSF
                     SFGWGAAAIAYGITFNLFEPDIAWRVCFFLGILPALVVVYLRRNLEEPELFLRSRARQ
                     NAASPAQSFMRVFRKPLIGKTVLCSLLSGGMLGAYYAIATWLPTFLKTERGLSVFGTS
                     SYLTVTILGSFVGYVAGAYATDRWGRRATYIVFAAGAFAMALAYMVIPVSNTSMLFLG
                     FPLGVLMQGVFSGIGATISESYPNDVRATGYGVSYNVGRVIGSLFPLSVGWLSSDGTS
                     LPLAIAMVAGVGYAMVILSAALLPETTGLELDEAAGEGVTVHGNGPQQAPAGRLA"
     gene            complement(6187..>6566)
                     /locus_tag="VLI90_11130"
     CDS             complement(6187..>6566)
                     /locus_tag="VLI90_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HSV14805.1"
                     /translation="RRRFAQAWQRFFCDYDVLLCPAAATSAFPLNESGEPWQRVLSVN
                     GNPQPMTTQLFWAGYSGLCGLPSTVAPIGPGRSGLPVGVQIVAPRFGDLTSLRFAQLL
                     ESSGHAFKPPPAVTPAAQGVHGR"
BASE COUNT         1290 a         2050 c         2097 g         1129 t
ORIGIN      
        1 ctggaggacg ctaaactcca agtggacgtc gcaggttctg cggcagaagc gatcaataag
       61 cttgcgctcg tccccggcag ctatgatgcc gtgatcatcg atgtcggctt acccgatcgc
      121 aaaggcgaca ctctgatcca cgaaatgaaa gcgatctacc cccatcttcc catcatcctc
      181 gcgaccggtc agaatacccg cgatctgcgc accaaattcg acgaccaaag cggattggct
      241 tttgtgagca agccttatac ggcgcaagaa ctgttgaatg cgttgcgcag cttgggagtt
      301 ggtatcggcc aatctgtgta ggttatccga gcgcctgaac acgctggccc ggccgagtcg
      361 taaccggggt tgtgactgtt gcggcgatca gcaagcgcct gtggtctgct gcatgacgcg
      421 gaccagatcg tcttcggaga atggctttcg gatcgtgggg aatccgcccc gtcgacgctc
      481 tcgccccgac acttggagaa ccttcaacgc ggggtgtagg cgagttgcgc gttcggccaa
      541 ctcatggcca tccatgctcg gcatatcgat gtcggtgatc aggatcgaga tcgaatcatc
      601 gcgctgcagt agctccaggg cttctgaacc gctgctggca cagattacat ggcagcccat
      661 atcctccagc atggtcgcga tcacatcgcg cacaccggca tcgtcatcga cgaggagtat
      721 cgtgtgtgtc attcgaaata aacaacccaa acctcttgct gttcccgctc gccgggatag
      781 gcggcgggaa cggcgggcat ggctgccagt tcactgttgt tcccgattga agtcgcgtcg
      841 atggagtcgt ggtatcctgg gtctcgaagg atccaccctt gcgtccttga tacggtgccc
      901 ggtgtttcgg ctgatctggt tgaagctgat cttgcaggaa tcaaatgtgg ccggatacca
      961 ggccgccggg caggtcactt tcccgttcgg cccgggcggc cattcaaaat actccagccg
     1021 ctacacaacg cggaattcaa gacgaacgaa tagggcgcgg tggtgcgacc gctacttcgc
     1081 atacttcccc gaaagctgcg gcgtcaggtc cggataaagg cccggcgtct tttccttggc
     1141 ttcttcgtgc atctggaggc gggtcttgcg cgcccaggtg cgcttgagat catccttgat
     1201 gccgaacttc aagcggccga ggccttcggt ttccagctcg atgctatcgc cgtccatgaa
     1261 cgggttgagg ccccggtgat tggtgccggt cgcgacgatg tcgccgggga gtagcgtgtg
     1321 gatgctggac agccaggcga tgcacttggc gatcttgtgg cccatgtcgt cggtgttgaa
     1381 gttctgcatc agcacgccgt tgttccacag cctgatttgc agtttgagcg ggtccttgat
     1441 ctcgtctgat gtgacgagcc aggggccgat cggcgcgaac gtgtcgcggc tcttcatctg
     1501 gaagaacacg ttggctggag gccccaggcc gcgggccgaa ccgtcgatga agttcatgta
     1561 gccgaagacg tggtccatcg cctcggcttc gctgacatgg ctgcacttct tgccgatgac
     1621 ggccgcgatt tcagcttcgc cctcgaagca ggtggccggc acgtccggca gtaccatgat
     1681 gtcaccatgc ccgatgatgg cgcctggcga cttgtggaag gcattgagcg gcgcgggcgc
     1741 gctgcgcgtg ccgtcttcca tgtaattgac cgccatgcag tcgatcgtgc gcggacccgg
     1801 caccggcgga cgaatgcgaa cgcttgacag cggcaatccg tcgccggacg ctgcggctgc
     1861 ttcgagccgc gccttgtagc tgctccaatg ttcgatcagc ccgttcatca catcgcccgg
     1921 gccggtgtgc gggatgtcct cgaccgcagc gctgacatcg accacgacgt cgcccttcaa
     1981 cacgccaagc ttgaaatcat cgaagtagag tatcttcatc gtgtgtttct caccccttgt
     2041 gtcgagcagg cagcgccgaa gccggtcgct tgcggcggtt ggtcgccgtg ccaaccttgc
     2101 gatgagctta taccgcttcg acgcgggagg agaacaggct tgttcaggtg tccggcccgc
     2161 gaggtgagcg tggctcgcgc cctcgacgcg agccacgccg tgcaggccgg aaggcttgac
     2221 tggctcgatc gcctcactca cgccgcgtga ggattcacga cggtgtaacc gcgaagcgag
     2281 cgggcatgta tccgcgtggc gtggccgcct gaattggcgt cgtaggccat ccaggtatcg
     2341 cccgcgactt gctgctccag aacgaagaca tgcccgcgcc gtgcggccac catgcccggg
     2401 gccggcgccg tgcggggaaa tcgcagccag ttcgccgcca ggttcaagcc gggaacgatg
     2461 cgcccgaaca cccgcaggga cgcgccgcat ccacagaacg aattcgggca accggccgga
     2521 cgaccgccga cgacccggcc gctgtcaaca ccggccatcg cgtcggcgct tgccatcgcg
     2581 ttccggtagt tgacgacggg ttgcgcagga acccgctgct ccatcatgcg ataggaagtc
     2641 cggatctgcg gttgccgcat tccggtgaaa tcacagggca tcgtgacatt gcattcccgt
     2701 ggtgcggaat acgaccggaa gggccgcgca ttggatgcgt ttgaaaaggt taccatgaaa
     2761 aaggttgcaa caaacaggac gcgaaacacc atgcagagct ccatcgaatg agcctgcccc
     2821 gcaacaaacg cagtgaatct ttgtggccag aatgagttgt cggattcccg gcgtgcctcc
     2881 ggggcacgcg gaactatcac gtcgccgtct ctagctctgc cgcggcggat cgaactcgct
     2941 cggttgcgcc tggtgcccgc gatattcggc gctgcgcttc gattgtgaga gcgccccaat
     3001 cgccaccgcc gccgccgcaa accccgacgc ggcacccacg cccagcgccc atcgcggccc
     3061 gagatgatcc gcgacccatc caacgatcgg ggcgccgacc ggcgtgccgc ctagggcgac
     3121 accgaggcgc agcgccatca cgcgtccgcg catcgcgggt tcggtcgaga gctgcatcag
     3181 cgcgttcgag gtgttgagca cggtcagcga ggcgacgccg atcacgacga gggcggccgc
     3241 gaacagccag tagttcggtg caagtgccgc gagggtgcag ccgaggccga aggtcgccga
     3301 gcccgccagc agtgaggtga agctcgggcg ttctcggctg gcggccagca gcgcccccga
     3361 catggtgccg atcgccatca tcgaggacaa gaggccgaag ccgcgcgcat cggtatggaa
     3421 gacgccgacg gccatggtcg agatgaagat cggaaagttg agcccgaacg tcccgatcag
     3481 gaacagcatg accagcgtcg ctcgcagatc ggggcgggac cagacgtagt gaaacccctg
     3541 cagcacgctg cccttggcgc ggatggcgcg tgcattgggg cggagctcgg aggtgcgcag
     3601 caggcacagc gacaccaaca ccgccatgta cgatgcgccg ttgaacagaa acgcccagcc
     3661 ggtgccgacg gcggcgatga ggaggccggc cgccgcgggg ccgatcatgc gcgcggcgtt
     3721 gaaggaggtt gagttcagcc cgatcgcgtt cgacaggtct ctttcgccga ccagctcggc
     3781 gacgaaagtc tggcgcaccg gcgcatcgag ggcggcgctg cagccgaaca ggaacgcgaa
     3841 tacatagaca tgccacagct gcaccaggcc ggtggtcgtg agcaggccta ggatcagcga
     3901 gagcgcgccc atcgtggctt gggttgccat caacagtttg cgttggttga aatgatcggc
     3961 ggcatagccg gaccacggca tcaacagcaa ctgcggcccg aactgcaacg ccatcacgat
     4021 gccgaccgcc gaggcgcttt gatgggtgag ctcggtcagg accagccagt cctgggcggc
     4081 gcgctgcatc caggtgccga tgttcgatac cagcgcaccc gctgcccata tccggtagtt
     4141 gtggttcgcc agcgagcgaa acgtgcggat catgtctgcc ggggttcatt gcccccgcgg
     4201 aaccttccga accagcgcgc caggaacagg ccgatgagaa gtccgccgag accgagtgct
     4261 gcgacgtctg cggtctctct cagggcgaaa tcgccgacgc ggcagaccag gagatatccg
     4321 atcacgaggt tgaagaagcc ccagaggaga ttgacgatcg aactcgacaa gccctggccc
     4381 ggcggctttg cgaagggaga ctggaatggc tctcccctca gaccggcgac gagatgggga
     4441 atcgcgttgg tgaggaaggc gccgccgaag aagtaggccg tgagctcaag ccagttcatt
     4501 ggttgggtcc tttcaagaag atccaggacg gcccgcatcg agccggtcgc cgacccggcg
     4561 cggggtcgat tcaacgccgg tgccggtcgc gacgcctgtt actacgacct gcgtgacgtt
     4621 tcggttcctg agctggttct ccagatcggt actcgcgaac gcgcccccac gggagaagct
     4681 tggtccagcc gtggagcagt tttgcaaatg gcgtggccgt tcggttcgtt tgggcgccaa
     4741 gcgtaatccg ccgtgaccgg cgaagatggc ggattacgcc tttggcctgc gagctactgg
     4801 gatggcgacg ctggaatctg ccccaccaac cggcgcagcc ccgtgatcac gcaagccgcc
     4861 cggcgggtgc ctgctgaggc ccgttgccat ggacggttac cccctcacca gctgcctcat
     4921 ccagttcgag gccggtggtt tccggcagca gtgcggcgga gagtatcacc atggcataac
     4981 caacgccggc caccatggca atcgccagcg gcagagatgt gccatcgctg ctcagccatc
     5041 ccaccgacag cggaaagagc gaaccgatga cacggcccac gttgtaggac acgccatagc
     5101 cagttgcgcg aacgtcgttc gggtacgact ccgagatagt cgcaccgatg ccggagaaca
     5161 ccccttgcat cagtactcca agcggaaagc cgaggaagag catcgacgta ttggagacgg
     5221 ggatcaccat atatgccaac gccatcgcga aggcgcctgc ggcgaagaca atatacgtcg
     5281 cgcggcggcc ccagcggtcc gtggcgtagg cgccggccac gtagccgacg aaggagccga
     5341 ggatggtcac ggttagatac gaactggtgc cgaagacgga cagaccgcgc tcggtcttca
     5401 ggaaggtcgg cagccaggtc gcgatcgcgt aatacgctcc cagcatgccg ccggacaaca
     5461 ggctgcacag caccgtcttg ccgatcagcg gtttgcggaa cacgcgcatg aagctctgcg
     5521 ccggcgacgc tgcgttctgc cgcgcgcggc tcctgagaaa aagctcgggt tcttccagat
     5581 tgcggcgcaa atacaccacc accaacgccg gcaggatgcc gaggaagaag caaacccgcc
     5641 aagcaatgtc cggctcgaac aagttgaaag tgatcccata ggcgatcgct gcggcgcccc
     5701 agccaaacga aaagctgctt gcggtaaaac cggaatattt gccgcggtgc gcggggttgc
     5761 ggatcatctc cgtcaccagc accatgcaca gcgacgattc gccgccgaac ccaagcccct
     5821 ggatggtgcg gaagatcata agctgctcgg gcgaattggc catgccgcac aggaaacacg
     5881 cgaccgcaaa cacgacgatc gtccagcgca gcacgcgcac gcgaccgtag cggtccgcca
     5941 gcagccccgc accgatcgcg ccgacgaggg acgatatcaa cgtccacgtg acgatggcgc
     6001 ccgccgccga tttgctcatg gcccactgcg taagcagggt ggggatcagg aacgaataga
     6061 tcatgaagtc gaagatgtcg acggcgtgtc cgaggaacgc gccgtagaag cccttgcgtt
     6121 ccatgcgtga caactctttg aaccaatcca gcattgtcgc ccccctgttg cggttgtacg
     6181 tggccttcac cggccgtgaa caccttgcgc cgccggcgta actgctggcg gcggcttgaa
     6241 tgcatgaccg ctactctcca gcagctgggc aaaacgcagc gacgtcagat cgccaaaacg
     6301 cggagcgacg atttgcacgc cgaccggtag tccgctgcgc ccggggccga tcggcgctac
     6361 ggtcgatggc agaccgcata ggccggaata cccggcccag aatagctggg tcgtcatcgg
     6421 ctgcgggttg ccgttcacgg acagcacgcg ctgccacggc tcacccgatt cgttgagcgg
     6481 gaacgcggaa gtggcggcgg ccgggcacag cagcacatcg tagtcgcaaa agaaccgctg
     6541 ccatgcctga gcgaagcgcc tgcgcg
//
LOCUS       DATIMH010000644         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2176880, whole genome shotgun sequence.
ACCESSION   DATIMH010000644 DATIMH010000000
VERSION     DATIMH010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2176880"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VLI90_11135"
     CDS             <1..1102
                     /locus_tag="VLI90_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSV14806.1"
                     /translation="ATAWDQNAGLFGATPISATLEEVSLGWLLDVLGLPATAGGAFVT
                     GATVANFTALAAARHALLARAGWDVEAQGLFGAPPIQVVIGEEAHPSVIKSLGMLGLG
                     RERVVRVPVDGQGRMRADALPTLSGPAIVCVQVGNVNTGAFDPVPAISAWAHDAGAWV
                     HVDGAFGLWAAASPRHAHLTAGVADADSWATDAHKWLNVPYDSGLAFVRDPADLRGAM
                     SLTAPYLPQGAHREPLMYVPELSRRARGVDVWAAMKSLGRAGLAELVDRNCRCATRFA
                     EIVQASGFEVLNEVVLNQALVAFGAPEITQRVIAAIQEEGTCWCGPTLWQGRTAMRIS
                     VSSWATRIEDAERSAQAIVRVARSVIGSATAA"
     gene            complement(1089..1553)
                     /locus_tag="VLI90_11140"
     CDS             complement(1089..1553)
                     /locus_tag="VLI90_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14807.1"
                     /translation="MKIIALALLLAGFALPAGDPTGFYLWKSAELKGYKTSLAAKMSD
                     QKLASQTIANYGNYLFMIAHREAPGEAEYHATQADVFMVQSGEATLTVGGELVNGKTT
                     AANEMRAPSIKGGIDKKLAAGDVVTIPAKIAHQLKIDSGKEFTYFVVKITQP"
     gene            complement(1594..>2355)
                     /locus_tag="VLI90_11145"
     CDS             complement(1594..>2355)
                     /locus_tag="VLI90_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSV14808.1"
                     /translation="GFGSGDIVVKRVWVLSPNHLQANIAVADNAVAGNSEVSVISGFQ
                     VLAQTNAFQVLPRNSTVPVITGVTNANTAQQTIYPGGFVTIYGANLAAVPSNVQVTLG
                     DQPMTLQPGGVTSGQINFFIPANFPSGVATLRVNNGVTAVTPIAVQIDVPPPAIQGVT
                     NASGVTYDATHPAAAQDVVNVLVSNLDPTVLANPGRLQLTLNGQTMAAQSVTPIGGGQ
                     TQITFVVTQSFGGVVVNLAVVVDGSSSSPYALAVR"
BASE COUNT          397 a          721 c          798 g          439 t
ORIGIN      
        1 cgccactgca tgggatcaga atgccggact cttcggcgcg actccaatct cggcgacgct
       61 ggaagaagtg tccttgggct ggctgctcga cgtactcggt ttgcccgcga cggccggcgg
      121 ggcattcgtg acaggcgcta cagtcgcgaa ttttaccgcg ctcgctgcgg cgcggcacgc
      181 gctgctggcg cgcgcgggct gggacgtgga agcgcaggga ctcttcggcg caccgccgat
      241 tcaggtcgtg atcggcgagg aggcgcatcc ttctgtgatc aagtcgctgg gcatgctcgg
      301 cctcgggcgc gagcgcgtgg tgcgcgtgcc ggtcgatggt cagggccgca tgcgcgccga
      361 cgcgctgccc acgttgagcg gtcccgcgat cgtctgcgtg caggtcggca atgtgaatac
      421 cggcgccttc gatccggtgc ccgcgatctc tgcctgggcg cacgatgccg gcgcgtgggt
      481 tcacgtggat ggcgcgttcg gactttgggc cgcggcgagt ccccgtcatg cgcatctgac
      541 cgcaggagtc gccgacgccg actcgtgggc caccgacgcg cacaagtggc tcaacgtccc
      601 ctacgatagc ggcctcgcct tcgtgcgcga ccccgccgac ctgcgcgggg ccatgagtct
      661 cacggccccg tacctgccgc agggagcgca tcgcgagccg ctgatgtacg tgcccgagct
      721 ttcgcgccgc gcgcgcggtg tcgatgtgtg ggcggcgatg aaatcgctgg gacgcgcggg
      781 gctcgcggaa ttggtggacc gcaattgccg gtgcgccact cgcttcgcgg aaatcgttca
      841 ggcgtcgggc ttcgaagtgc tcaacgaggt ggtcctgaat caggcgctgg ttgcgttcgg
      901 agcccccgaa atcacgcagc gtgtgatcgc ggcgattcag gaggaaggta cctgctggtg
      961 cgggcccacg ctgtggcagg gacgcaccgc catgcgaatc agcgtgtcat cgtgggccac
     1021 caggattgag gacgcggagc gaagcgcgca agcaatcgtg cgcgtcgcgc gcagcgtgat
     1081 cgggagcgct acggctgcgt gattttaacc acgaagtacg tgaactcctt gccgctatcg
     1141 atcttcagct gatgcgcgat tttcgcggga atggtgacga catcgccggc cgcgagcttc
     1201 ttatcgatgc cccctttgat tgaaggagcg cgcatttcgt tggcagcggt cgttttgcca
     1261 ttgacgagtt cgccgcccac ggtgagcgtg gcctcgccgc tctgcaccat gaagacatcg
     1321 gcctgcgtgg cgtggtactc ggcctcgccg ggggcctcgc ggtgggcaat catgaacagg
     1381 taattgccgt agttggcgat ggtctggcta gcgagcttct ggtcgctcat cttggccgcg
     1441 agcgaagtct tgtagccctt cagctcggcg gatttccaaa gatagaaacc ggtaggatcg
     1501 ccggcgggca aggcaaagcc ggcgagcaac aaggcaagcg cgatgatttt catgcagaca
     1561 cagtatcacg aaccggaagg ttcgagcccg cggctaccga accgcgagcg cgtacggcga
     1621 actgcttgag ccatccacta cgaccgccag attaaccacc acgccgccga agctttgcgt
     1681 gacgacgaag gtgatctgcg tctgtccgcc cccgatcggc gtcacgctct gcgctgccat
     1741 cgtctggccg ttgagcgtga gctgcaagcg gccaggattg gcgagcaccg tcgggtcgag
     1801 attagagacg aggacgttga ctacgtcctg cgcggcggcc gggtgagtcg cgtcgtatgt
     1861 cacgccgctg gcgttggtca cgccctgaat ggcgggcggc gggacatcga tctgcacggc
     1921 gatcggagtg acggcggtta cgccgttgtt cactcgaagg gttgcgacac cgctcgggaa
     1981 attcgctggg ataaagaaat tgatctgacc ggaagtgact ccgccgggct gcaaagtcat
     2041 cggctgatcg cctaacgtca cctgtacgtt tgacggtaca gccgccagat ttgcgccata
     2101 gatcgtcacg aagccgcccg ggtagatggt ctgctgcgca gtgttggcgt tggtcacccc
     2161 ggtaatcacc ggcacggtcg aattacgtgg cagcacctgg aacgcattcg tctgcgcgag
     2221 cacctggaac ccggaaatta cgctcacttc ggagttgccc gcgaccgcgt tatcagccac
     2281 cgcaatgttg gcctgcagat gattggggct cagcacccat acgcgcttca cgacgatatc
     2341 gcccgagccg aagcc
//
LOCUS       DATIMH010000645         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3046340, whole genome shotgun sequence.
ACCESSION   DATIMH010000645 DATIMH010000000
VERSION     DATIMH010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3046340"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VLI90_11150"
     CDS             complement(<1..1435)
                     /locus_tag="VLI90_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HSV14809.1"
                     /translation="MEPDAYRYTPKREDPAVAQQRDRDERAHARRASGNVGLTGRLTL
                     AFAALITLAIGASCWIFADQSGAQLDDMMGEQARLVAYTIAQAAPAPLMEQKTDALRQ
                     IGHSLLNTRDVLYVAFVDAQKKPLALANRYVDFTWDDVSPIGPDALSLHMVAAGSGKT
                     FGDYVNVFAPVVAPLNAIAAPAALSNKTRVKSATTAASKSRLLGFVVVGVSLDREHAQ
                     LRWINWLIAGIGLTLVLISVPVAYILVYGVLKPIRQLVSATREIALGRFNVEIQIDRD
                     DTIGELAESFQDMVNQVRRQREDLAQTNVKLADANGLLGEANQKLAEANRDLEQKVSE
                     RTAQLATANHRLSAEIAEKEDFLRAVSHDLNAPLRNIAGMAGMLLSKHGERFDEDITH
                     RLQRIQKNVEAETDLIGELLELSRIKSRRLKLEPVDLDALVRDIGEVFEEDLRSRGIQ
                     LMLDAPLPVITVEKARIRQVFQNLIDNA"
     gene            complement(1703..2842)
                     /locus_tag="VLI90_11155"
     CDS             complement(1703..2842)
                     /locus_tag="VLI90_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14810.1"
                     /translation="MKRQTGRTTAWVVALLGGSCAGPARVEPPPLHREPIVVQLRRAE
                     PLLAQYPYSPLLDFESDADLVFVSDATRLANSSAHTGDHAAIIPAGATQWAVKLDTLM
                     MGRDFPANWTLAAAYFRAPQRTAITAGIERGLGQKPSLPRDVVPGRWTSVFAELSSAS
                     ASGGDLVFHFEPPAPTDLLCDDLVLIDNHRTFAPGVRQAGFHLVIERAGQPPLDLPTE
                     QASIDGWRIVQVSAMRVLLASDGRAKRQAIYADGRVFLDGSPSENVGTIACAPEQGRV
                     DRATDGDANNDGYNECRGAYEVVAATPRVDVTLTPASSDSPLDRPVLEIANLPAGALT
                     ATMQGQWLEHVERLANGNVLVELPGKIARAVTVTISVSAGVTSVQ"
BASE COUNT          492 a          922 c          992 g          551 t
ORIGIN      
        1 tcgcgttgtc gatcaggttc tgaaagacct ggcggatgcg cgctttttca acggtgatga
       61 ccggcagcgg cgcgtcgagc atcagctgga tgccccggct gcgcaggtct tcctcaaaca
      121 cttcgccgat gtcgcgcacc agcgcatcga gatcgaccgg ttccagcttc aggcgtcgcg
      181 acttgatgcg cgacagctcg agcagttcgc cgatcaggtc cgtctcggct tccacgtttt
      241 tctggatgcg ctgcaggcgg tgcgtgatgt cttcgtcgaa gcgctcgccg tgcttgctga
      301 gcaacatgcc ggccatgccg gcgatgttcc gcagcggcgc gttcaagtcg tgcgacacgg
      361 cgcgcaggaa atcctccttc tcagcgatct cagcgctgag ccgatggttc gccgtcgcca
      421 gctgggcggt gcgctcgctg accttttgct cgagatcgcg attggcctcg gccagtttct
      481 ggttggcttc gccgagcaac ccgttggcgt cggcgagctt cacgttggtt tgcgccaggt
      541 cctcgcgctg ccggcggact tggttgacca tgtcctggaa cgactcggcc agctcgccga
      601 tcgtgtcgtc gcggtcgatt tggatttcga cgttgaatcg ccccagcgcg atctcgcgcg
      661 tggccgacac cagttgccgg atcggcttga gcacgccgta caccaggatg tacgccaccg
      721 gcacactgat gagcacgagc gtgagaccaa tgccggcgat gagccagttg atccagcgga
      781 gctgcgcgtg ttcgcgatcg agcgatacac cgacgacgac gaaaccgagc aaccgcgatt
      841 tcgacgccgc ggtcgtggcg gacttcacgc gcgtcttgtt cgataaagcg gccggcgcgg
      901 cgatcgcatt caatggcgcc acgaccggcg cgaagacgtt gacgtaatcg ccgaacgtct
      961 taccgctgcc agcggcgacc atgtgcagcg acagcgcgtc gggcccgatc gggctgacat
     1021 cgtcccaggt aaaatccacg tagcggttgg ccagcgccag cggttttttc tgggcatcga
     1081 cgaacgcgac gtaaagcacg tcgcgcgtgt tgagcaggct gtgaccgatc tggcgaaggg
     1141 cgtcggtctt ctgttccatc agcggcgccg gcgccgcttg ggcaatcgtg taagcaacga
     1201 gccgggcctg ttcgcccatc atgtcatcga gttgcgcgcc gctctggtcg gcgaagatcc
     1261 aacagctggc gccgatcgcg agcgtgatga gcgctgcgaa cgccagcgtc aatcgaccgg
     1321 tgaggccgac gttgccgctg gcgcggcgcg cgtgggcgcg ctcatcgcga tcgcgctgtt
     1381 gcgcgacggc ggggtcctcc cgtttgggcg tgtagcgata agcgtcgggt tccatgccac
     1441 gcgtgcgggc caaagtgcag cccgcaccga tgaacatcga cgcgatgagc ggcgcggttt
     1501 atccgggcgt tatatcaaga ttttgatata ggtgaattga gcttgagtgt tggcaaaatg
     1561 gcgagtttgg gagagatggg gataaaatcg aataaagcgc aacatgaaag cggagttttt
     1621 tgccgtatct attttttata ccggatgtta tgtgatattc acccgccata tcgccggatt
     1681 gttgcgcggc tgagtccgtc cgctactgca cgctcgtgac gccggcggaa acgctgatgg
     1741 tgaccgtgac cgcgcgggcg atcttccccg gtagctcgac gagcacgttt ccgttcgcga
     1801 gccgctcgac atgctccagc cactgccctt gcatcgtcgc cgtgagggcg ccggccggca
     1861 ggttcgcgat ctccagcacc ggccgatcca gcggagagtc gctgctggcc ggcgtcaacg
     1921 tcacatccac ccgcggcgtg gccgcgacga cctcgtacgc cccgcggcat tcgttgtagc
     1981 catcgttgtt ggcgtcgccg tcggtggcgc gatcaacccg tccctgctcg ggtgcacagg
     2041 cgatcgtgcc aacgttctcg ctcggcgagc cgtcgagaaa aacgcgaccg tcggcgtaga
     2101 ttgcctgacg cttggctcgc ccgtcggatg caagcagcac gcgcatcgcg ctcacttgga
     2161 cgatgcgcca gccatcgatc gacgcctgct cggtcggcag atcgagcggc ggttgaccgg
     2221 ctcgctcgat caccaggtgg aagcccgcct gccgcacgcc gggggcgaac gtgcggtggt
     2281 tgtcgatgag cacgaggtcg tcgcagagca gatcggtcgg cgcgggcggc tcgaagtgga
     2341 aaacgagatc gccgccgctg gcgctagcgc tggacagctc ggcgaacacc gaggtccatc
     2401 gaccggggac gacatcgcgc ggtagcgacg gcttttggcc caggccgcgc tcgatgccgg
     2461 cggtgatcgc ggtgcgctgc ggcgcgcgga agtaagccgc cgccaacgtc caattcgcgg
     2521 ggaagtcgcg acccatcatt agcgtgtcga gcttcacggc ccactgcgtc gcccccgcgg
     2581 ggataatggc cgcgtggtcg ccggtgtgcg cactgctgtt ggcgagacgg gtggcgtcgg
     2641 agacgaacac cagatcggcg tccgattcaa aatcgagcag cgggctgtaa ggatattgag
     2701 ccagcagcgg ctcggcgcga cgaagctgca caacgatcgg ctcccgatgc agcggcggcg
     2761 gttcgacgcg cgccggcccg gcgcagctgc cgccaagcag cgcgaccacc cacgccgtcg
     2821 tccgtcccgt ttgtcgcttc actggagttg cgtatcgtgc ggacgacggt gggcgtgtca
     2881 atgtgctcga gaatttgata gccgggggcg aaagccccgg tctgttcgaa cggaaaagga
     2941 ccggggcttc cgccccc
//
LOCUS       DATIMH010000646         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3623211, whole genome shotgun sequence.
ACCESSION   DATIMH010000646 DATIMH010000000
VERSION     DATIMH010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3623211"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..440)
                     /locus_tag="VLI90_11160"
     CDS             complement(189..440)
                     /locus_tag="VLI90_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14811.1"
                     /translation="MAERIGGIIEQHIRKRKFAMIEIELADGRVVKIRENDQAWVEHD
                     AGLLYIVASPNVTDGSLTEIIVLQHVAMVRIRERLPSQT"
     gene            complement(498..926)
                     /gene="rplK"
                     /locus_tag="VLI90_11165"
     CDS             complement(498..926)
                     /gene="rplK"
                     /locus_tag="VLI90_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSV14812.1"
                     /translation="MAKKEITVQFKLQAPGGTATPAPPIGPALGQHGINPGQFIQRFN
                     AMSAHLKGKVIGVLVTAYKDRTFDIELKSPPASVLLKDAVKAEKGSGEPNKTKIGKVT
                     MAQCKAIAKEKIKDLNAFDEDHAARIIAGTARSMGITVEG"
     gene            complement(959..1255)
                     /locus_tag="VLI90_11170"
     CDS             complement(959..1255)
                     /locus_tag="VLI90_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14813.1"
                     /translation="MPDFFQYYGRAQSLRGSFGSLPPWARSIVGLFAIPGIVLACLSL
                     LAFIVSILALLLLTVPVYRILQALTGGGGGFSTTAMADPDPSPGRKQVDVRVVD"
     gene            complement(1365..2687)
                     /locus_tag="VLI90_11175"
     CDS             complement(1365..2687)
                     /locus_tag="VLI90_11175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV14814.1"
                     /translation="MRVLLLANDCNPDWPSLPVVGYNACRGIAEHVDVVVATHVRNKP
                     NIDKRGFGRAEVVYLDNEYVASPMYKLATFLRGGSSVGWTTNIAMQYPPYVAYEWEAW
                     KRFKPQLKAGEFDLVHRVTPMSPTLPSPMAKWCKRIGVPFVLGPLNGGLRWPPEFTGE
                     LKKEKEWLTYLRDAYRLLPYASATYSKSSAILAAFQHTIDRLPARVRDRIFDFPEVGI
                     DPALFEAPAERPVRERVTFLYAGRLVPYKCPDVAVLAFASNPNLHKHKLIVAGDGPEM
                     PRLREIIQQHKLEGCVELLGRRTQAEVGQLMRSADVFTFPSIRELGAGVVVEAMGCAL
                     PCIVVDYGGPGGLIHSQSGVKVSLGDKTLLINRFAREMESLANDPERRRRFAERGHRD
                     AMQIYSWTNKGRKTADVYEWVLGRRGTKPDFYADGTAVDQISPEPAMT"
     gene            complement(2970..>4188)
                     /gene="priA"
                     /locus_tag="VLI90_11180"
     CDS             complement(2970..>4188)
                     /gene="priA"
                     /locus_tag="VLI90_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HSV14815.1"
                     /translation="REASGSSEYPSTGSGQALRTRREGTSKYHLLILPSRVRGLQLPH
                     VELIDMRQENKFRRGVHLLSQRLEHLLRTTVEAGRQAILMLNRRGYSNFVYCSSCGNP
                     IQCKYCDATMTYHRSAGAEVKTARLEAGLHTGQLHCHYCLAVNQLPPACPTCGKKLSL
                     FGLGTQRVEEEISKKFPDLKFARVDSDSMRSSKDYEALLGRFAKGEVQVLLGTQMIAK
                     GLDYPNVTLVGVISGDTALALPDFRAAERTFQLITQVAGRAGRGDSPGRVVLQTFLPD
                     DPTIQFAIKQDFLNFAKAELKHRQSVGLPPFTRMVRIILRDQEAEKLHKLSEQLAAAV
                     NNAVAKVGEDVRVKGPMPCAISRIAGYFRNQIVMTSSRAESLQKVIAAVREAKALSKS
                     DRVAVDVDPVSLL"
BASE COUNT          812 a         1422 c         1151 g          803 t
ORIGIN      
        1 tacgaactcc gaatcaaact taggattcgt cgcgacaaac atcgtgcgct tgcccggatg
       61 aatccaagcg aaatccgagg acttcaccgg gatgacgtca ccgtcagacg tatggatatc
      121 gaacggcacg aatggccgtt cgtccagcat cgtctttagg cggtcgtaca tcatcttatt
      181 gaatggctct atgtttgtga cggcagccgc tctcgaatcc gtaccatcgc cacatgctgc
      241 agaacgataa tttccgttag cgaaccgtct gtcacattgg gcgatgcgac gatgtagagc
      301 aatccagcat cgtgctcaac ccaggcctga tcgttctcgc gaatcttcac gacccggcca
      361 tcggccagct caatctcaat catcgcaaac ttacgcttgc gaatgtgctg ctcaattatg
      421 ccaccaatgc gctcagccaa cctcccgtgc tcccccaagg tattaataat accagatgag
      481 gcggccgtcg ggacggtcta cccttcaacc gtaatcccca tcgaccgcgc cgtccccgcg
      541 atgatgcggg ccgcgtggtc ttcgtcgaag gcgttcaaat ccttgatctt ctccttcgcg
      601 atcgccttgc actgggccat cgtgaccttg ccgatcttgg tcttgttcgg ctcgccgctg
      661 cccttctcgg ccttcaccgc gtccttcagc agcacgctgg ccggcgggct cttcagctcg
      721 atgtcgaacg tgcggtcctt gtacgccgtc accagcacgc cgatgacctt gcccttcagg
      781 tgggcactca tcgcgttgaa gcgctggatg aactgccccg ggttgatgcc gtgctgaccg
      841 agcgcgggcc cgatcggggg cgccggcgtt gccgtgccac caggagcctg aagcttgaac
      901 tgaacggtga tctctttctt ggccatggtt tacgctcgca tcacgatgtc agacgactct
      961 aatcaaccac tcgcacatcc acctgtttac gtcccggtga cgggtcggga tcggccattg
     1021 cggtggtcga aaatccacca ccgccgccgg tcaacgcctg aaggatccga tacacgggaa
     1081 cggtcaggag taaaagggcg aggatactaa cgataaacgc cagcaatgac aagcacgcca
     1141 gcacaattcc gggaatcgcg aaaaggccga cgatggaccg ggcccaaggg ggcagcgagc
     1201 cgaagctccc gcggagggac tgggcgcggc cgtaatattg gaagaaatcc ggcatctgcc
     1261 cggattatag ggattcgtcg ccggttagtc agatgccgct tgcgccttag cgtctgtttc
     1321 ttccaacgcc ggaaccggcg ctaagccgca agcggcgaac acgcctacgt catggccggc
     1381 tctggcgaga tctgatccac cgcggtgccg tccgcgtaaa aatccggctt cgtcccccgc
     1441 cggcccagca cccactcgta cacatccgct gtcttgcggc ccttgttcgt ccacgaatag
     1501 atctgcatcg catcgcgatg cccgcgctcg gcaaaccgcc gacggcgctc gggatcgttc
     1561 gccagcgatt ccatctcgcg cgcgaaccgg ttgatcagaa gcgtcttgtc ccccagcgac
     1621 actttcacgc cgctttggga atgaatgagg ccacccggcc cgccgtaatc gacgacgatg
     1681 catggcagcg cgcagcccat cgcctcgaca acgacgccag cgcccaactc gcggatggac
     1741 ggaaacgtga acacgtccgc cgagcgcatc agctgtccca cttccgcctg cgtccgccgg
     1801 ccgagcagct cgacgcagcc ctccagtttg tgctgctgaa tgatctcccg cagccgcggc
     1861 atctccgggc cgtcgccggc gacgatcagc ttgtgcttgt gcaaattcgg attcgacgcg
     1921 aatgccagca ccgcgacgtc cgggcacttg tatggcacca gccggcccgc atagaggaat
     1981 gtcacgcgct cgcgcactgg ccgctcggcc ggcgcttcaa acaatgccgg atcgatgccg
     2041 acttccggaa agtcgaagat ccggtcgcgc acgcgcgccg gcagccgatc gatcgtgtgc
     2101 tgaaaggccg cgaggatcgc cgacgatttc gaatacgtcg ccgacgcgta cggcagcaac
     2161 cgatacgcat cgcgcagata cgtcagccac tccttctctt tcttcaattc ccccgtgaac
     2221 tccggcggcc agcgcaggcc gccgttcaac ggccccagca cgaacggcac gccgatccgc
     2281 ttgcaccact tcgccatcgg gctcggcagc gtcggtgaca tcggcgtgac gcggtgcacc
     2341 aggtcaaatt cccccgcctt cagctgtggc ttaaatcgct tccacgcttc ccactcgtac
     2401 gctacatacg gcggatactg catcgcgata ttcgtcgtcc agccaaccga cgatccaccc
     2461 cgcagaaacg tcgccaactt gtacatcggc gacgcgacat attcgttgtc gaggtaaacc
     2521 acctcggccc gcccgaaacc ccgcttgtcg atgttcggct tgttccgcac gtgcgtcgcc
     2581 acaacgacgt ccacatgctc cgcaatcccc cggcaagcgt tgtatcccac caccggcaac
     2641 gacggccaat ccggattgca atcattggca agcaacagca cgcgcatagg cgggacaccg
     2701 taaaggaaca aagcttaaac ttccatcggc aggttgattg attgacgtta cggataactt
     2761 gatgctggtt actcggggac cttcgactgc gccgcatcca ttccggagcc cgcgagcgca
     2821 accatctttg ttccggtagc cgcggctctt cggcgccgac gcaaattgat tcggtgggtg
     2881 taacgcggct ttatggctct caagccgact attctgaaca tgcatgagga cgcgcccgcc
     2941 atgggcaagg ttgcccatgc gacggacttt cacaacaacg acaccggatc cacatccacc
     3001 gccacgcgat cgctcttgct cagcgccttc gcctcgcgca ccgccgcgat caccttttgc
     3061 agcgactccg cccgcgagct ggtcatgacg atctgattcc gaaaataccc cgcgattcgg
     3121 ctgatcgcgc acggcatcgg ccccttcacc cgcacgtctt cccccacctt cgcaaccgcg
     3181 ttgttcacgg ctgccgcgag ctgttcgctc agcttgtgca gcttctccgc ctcctggtcg
     3241 cgcaggatga tccgcaccat ccgcgtgaac ggcggcaggc ccaccgactg gcggtgcttc
     3301 aactccgcct tcgcgaagtt caaaaagtcc tgcttgatgg caaactgaat cgtcggatcg
     3361 tccggcaaaa acgtctgcag cacgacccgc cccggtgaat cgccgcgacc ggcgcgacct
     3421 gcgacctgcg tgatcagctg aaaggtgcgc tcggccgcgc gaaagtccgg cagcgccagc
     3481 gccgtgtcgc cgctgatcac accaaccagc gtgacgttcg gataatccaa ccccttggcg
     3541 atcatctgcg tgccgagcag cacctgcact tcgcccttgg cgaatcggcc gagcagcgct
     3601 tcgtaatctt tcgatgatcg catcgaatcg ctatcgacgc gcgcgaattt cagatccgga
     3661 aacttcttcg agatctcctc ttcaacgcgc tgggtgccga gtccgaacaa gctgagtttc
     3721 ttcccacacg tcggacacgc cggcggcagc tggttcaccg ccaagcaata gtggcagtgc
     3781 agctgccccg tgtgcaggcc tgcttcgagc cgcgcggttt tcacctccgc gcccgccgag
     3841 cggtggtacg tcatcgtcgc gtcgcagtac ttgcactgga tcggattgcc gcacgaggag
     3901 cagtagacga agttcgagta gccgcgacgg ttcagcatca agatcgcctg ccgccccgct
     3961 tccacggtgg tccgcagcaa atgctcgagc cgctgcgaca acagatgcac cccgcggcgg
     4021 aatttgttct cctgccgcat gtcgatcagc tcaacgtgcg gcaactgcag gccgcgcacg
     4081 cgcgacggga gaatcaacag atgatatttg ctggtgccct cacgccgggt tcgcagggcc
     4141 tgccctgagc ctgtcgaagg gtactccgac gacccggatg cttctcgc
//
LOCUS       DATIMH010000647         8229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1316640, whole genome shotgun sequence.
ACCESSION   DATIMH010000647 DATIMH010000000
VERSION     DATIMH010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316640"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..577)
                     /locus_tag="VLI90_11185"
     CDS             complement(98..577)
                     /locus_tag="VLI90_11185"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HSV14816.1"
                     /translation="MPELPPHRLSVALFQPQIAPNTGNIARLCVATGTRLHLVRPMGF
                     VLNDRELKRSAMDYWPRLQLTVHDDNDAFFRSMVDVRFWLFTSKATRAHWDAGFCDGD
                     CLIFGSETAGLPESLLTLHSQNTLRIRQMPGERCLNLATAVGIGLYEALRQVGSASS"
     gene            704..916
                     /locus_tag="VLI90_11190"
     CDS             704..916
                     /locus_tag="VLI90_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14817.1"
                     /translation="MSKPKLSLIVALLLAFCFAGISSEATAAPAGGAIITLVKHVHRK
                     PHKRRHKHRRHKHKHKMKTLGTLPKR"
     gene            1005..1784
                     /locus_tag="VLI90_11195"
     CDS             1005..1784
                     /locus_tag="VLI90_11195"
                     /inference="COORDINATES: protein motif:HMM:NF022838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HSV14818.1"
                     /translation="MKVAAFLILLCIAASASFAAPPVATSEPADALPEAVRASVTKHL
                     PDGKIVSFEHHVEDRRHLYFVDVKRATGDAVTLLASGRGQYLGLVEEDEDGDDSGDVF
                     IDLDTAPGGVKDAVAKYFGNAEVDSLFMEVEAERFIYCAEQTTGNTTRWASFALQGAL
                     VSEETEVPVDQLPAAVKDAVTKAHPVAKMDSASLVKEKEPAKTYYVVDIANSKGVKLE
                     LTVATEGTIQSSDPIDAADAAPEPAPTEPPPVKKPQRRPRG"
     gene            complement(1903..2700)
                     /locus_tag="VLI90_11200"
     CDS             complement(1903..2700)
                     /locus_tag="VLI90_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSV14819.1"
                     /translation="MHWIIGDVHGMLRPLRRLLDAVNADDPAARLIFAGDYVNRGPDS
                     SKVIDLLLTLDRAHFIRGNHDDVFDLVLHGHSYAESAASANPVIAFQWFMDYGMDSTF
                     YSYGADWHWLNETARSPTPDRMRQLTQFVPDDHKRFIHELPPVFEHEKFFVLHGRWSP
                     TTTCYPPTISEYLRVEPDMRQDVLWGRFQLEGIQGEKNWGKRGFFGHTPVHSYYQDPR
                     KAPMTPIGGPQIVLLDTAAALVSWGRLTAYCVEEERYIQTTHFGELV"
     gene            2774..3142
                     /locus_tag="VLI90_11205"
     CDS             2774..3142
                     /locus_tag="VLI90_11205"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002019.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HSV14820.1"
                     /translation="MPARKTVYPKNPIADELLKKYSKPMKPGSGALSAKDVANRLKGL
                     NGWTAADGRISKTYKFDDYYRTITFVNAVAWLANKEDHHPDLEVGYNQCVVHYSTHSV
                     GGLSENDFICAAKVDALLRL"
     gene            3404..4774
                     /gene="nuoH"
                     /locus_tag="VLI90_11210"
     CDS             3404..4774
                     /gene="nuoH"
                     /locus_tag="VLI90_11210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSV14821.1"
                     /translation="MSFGIPILAETSVSPILVEKLIFGDDPSFLWHVIYDLAIVAVVF
                     LPLISVIAMISIWAERRVAGRMQSRLGPNRVGPFGLLQSVADGLKLLLKEDLLPDEAD
                     GFLFHVAPYLAFAPVFAAYLALAFGPNLTFEPRLNVGVFWLLAILSVEIMGVILAGWA
                     SNNKWAIYGAMREACQMVSYEIPLGISIIIGVMTAGTLNLVQLGHLQSGGIHTWIIFR
                     NPFVFAAFFLYFVASLASNKRAPFDLPESESELVAGFHTEYSGLRFSFFFFAEYAAMF
                     VVAGIQTGLFLGGWNDPFGLLGYYYHRFTADPENVNTAGLIAVNLVGCAIFMTKCITI
                     IFVQMWIRWTFPRPRIDQVLYACVKVMLPLACLLLLGATLWQLFVPEISGIPWSNNTN
                     PWIPPQHEIFAWLVQVLLALIGVAGFSAVVGWVAYAFASGRGLKQRLTDPAPIVTEAD
                     ALVTQT"
     gene            4831..5430
                     /locus_tag="VLI90_11215"
     CDS             4831..5430
                     /locus_tag="VLI90_11215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSV14822.1"
                     /translation="MKAILPILATTDADMAGAVLFYLFALMAGGSAIGVVISRNIVRT
                     AVFLLFTLTGVAGLYFLLNAEFLAAVQLVVYAGGTLILIVFGVMLTSKSPFSRFEPKL
                     VEVIVALSIAVILLTALIMAIHRTPFPQSADVATDGYPMAQLGQALLGDYLVPFELAS
                     VLLLVVMIGAAYLAKGRRREEGTPAQSQSRLSSQARQWS"
     gene            5476..5829
                     /gene="nuoK"
                     /locus_tag="VLI90_11220"
     CDS             5476..5829
                     /gene="nuoK"
                     /locus_tag="VLI90_11220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSV14823.1"
                     /translation="MNVLAQSNFATIGLSHYLPLAAVMFVCGLLSILLKRNAIGILMG
                     VELILNAANVNLVAFNRFSYAGTSGSPLLTGQIFAAFVIVLAAAEAAVALAIFLNFYN
                     NFSTIDVERAQNLRG"
     gene            6036..>8229
                     /gene="nuoL"
                     /locus_tag="VLI90_11225"
     CDS             6036..>8229
                     /gene="nuoL"
                     /locus_tag="VLI90_11225"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSV14824.1"
                     /translation="MPTPATLLTIATLLPLAAFVLLVFVGKRMGTPLAGYVGTAFIGA
                     AFVCSIIAMISWYDGGMYAGRQWGFEKGPINIPIQWIPIGTPDKPSGIDQDHPGFLDL
                     GIFVDSLTITMFAMITLVSTLVFIFSIGYMAEDKRFPRFFTYLGLFCFSMLGLVIGGT
                     TLQLFIFWELVGLCSYLLIGFWYEKKSASNAAIKAFIVNRVGDFGFLIGFGILFYHIG
                     NVTLPHMWVSLGNAGSGLPITLSSGVVFSSGLLSVMGIGLFFGAVGKSAQFPLHVWLP
                     DAMEGPTPVSALIHAATMVAAGVYLVGRIFPLLTPDAKLFIAIIGCTTLTMAALIAIA
                     QTDIKKVLAYSTLSQLGYMILAMGVGSWVGGLFHLITHAFFKALLFLGSGSVIHAAHH
                     EQEMPKYGGLIRKIPVTGITFGIAVLAISGVGFGQFGFSGFYSKDMILAHAGAFATLA
                     HVVGKSKAYYLLFALPTIIAFVTPFYMTRCWMMTFAGKPRDEHLYEHAHESPIMWGPL
                     VVLAILAVISGKWMSIQEMIEGSIQENTAYCRGIDPSFHGFGTAWPSRVAENIGLEVR
                     DIAEVPPGTPSVPHGAPPAVAMTEAQHAHVIGHEMVEKWLGFWGFTSGILAGVIVYWR
                     GLGVAQAVLKFPPARWVRNWLYGRMYFDELYFYVFVNFVMAMSWLAGWFDRIVVDGIV
                     NGAARMVREMAMLAGLNDRYVVDGTVNGVGELAHSLGAAVRAPATAGRV"
BASE COUNT         1472 a         2490 c         2638 g         1629 t
ORIGIN      
        1 gcgaaaccgc aagcgactgc cgcgccgtct ttcacgaacg agcagtttac cgacgcctcc
       61 gccagatcct tcgactccgg cggagtaccg cctgcgctca ggatgacgcg ctgcccacct
      121 gccgcagcgc ctcatacagc ccgatcccca ccgccgtcgc caggttcaaa caacgttccc
      181 ccggcatctg ccgaatgcgc agcgtgttct gcgaatgcag agtaagcaac gactccggca
      241 gccccgcggt ctcgctgccg aagatcaggc aatccccgtc gcagaacccg gcgtcccagt
      301 gcgctcgcgt cgctttcgat gtgaacagcc agaagcgaac gtccaccatg gaccggaaga
      361 atgcgtcgtt gtcatcgtgc accgtcagct gcagccgcgg ccaatagtcc atcgcggacc
      421 gcttcagctc tcggtcgttc aacacgaatc ccattggccg gaccaggtgc aagcgcgttc
      481 cggtcgcaac gcaaaggcgc gcgatgttcc ccgtgttcgg cgcaatctgc ggctgaaaca
      541 gcgcgacgct gaggcgatga ggcggaagct ccggcatgcg gagcatcatc catcgaccca
      601 gcatcccaag ccagcgttcc ccgtacttcc cgtacttcct tgaatttccg ccggaagcac
      661 gctaattttc tgatattcaa attgaacgaa gggccacttc atgatgtcca agcccaagct
      721 gtcgttgatc gtcgcgttgc tgctggcgtt ttgcttcgcc ggcatttcgt ccgaagccac
      781 cgcagccccc gcggggggcg cgatcattac gctcgtcaag cacgtgcacc gcaagccgca
      841 caagcggcgg cacaagcatc gccggcataa gcataagcac aagatgaaga ccctgggtac
      901 gctgccgaag cgttgaatgt gggcgcggcc ggatccgggt cgtcgcgggt acgctccgaa
      961 cccggcgtgg gggaactcca acgatttggt cacacgagcc gacgatgaag gttgcggcgt
     1021 ttttgatcct gctatgcatc gccgccagcg cgagcttcgc cgcgccgccg gtggcgacga
     1081 gtgagccggc ggacgcgctg ccggaggccg tgcgcgctag cgtcaccaag catcttcccg
     1141 atgggaagat cgtgagcttt gagcatcacg tcgaggaccg ccggcacctt tacttcgttg
     1201 atgtgaagcg cgcgaccggc gatgccgtca cgctgctggc gtccgggcgg gggcagtatc
     1261 tcgggctcgt ggaagaagat gaagacggcg acgacagcgg ggatgtcttc atcgacctcg
     1321 acacggcccc gggcggggtg aaggacgcgg tggccaaata tttcggcaac gccgaggtcg
     1381 actcgctgtt catggaagtc gaggccgagc ggttcatcta ctgcgccgag cagacgaccg
     1441 gcaacaccac gcgctgggcc agcttcgcgc tgcagggcgc gctggtcagc gaagagaccg
     1501 aggtgccggt cgaccagctg ccggcggcgg tgaaggacgc ggtcaccaag gcgcacccgg
     1561 ttgcgaagat ggacagcgcc agccttgtga aggagaagga gccggcgaag acctactacg
     1621 tggtcgatat cgccaactcc aagggcgtga agctggagtt gacggttgcg acggagggga
     1681 cgatccaaag cagcgatccg atcgacgcgg ctgatgcggc gccggaaccg gcgccgactg
     1741 aaccgccgcc ggtgaagaaa ccccaacgac gtccgcgggg ttgagaagct cgcccgacgc
     1801 cgcggtctga ggtactcaga agacgcgggt acgggcgcgc gaattacccg ggtcttcgga
     1861 gtagctcaga caccggcgtc ggggggttgt gcgtaatacc tatcacacca gctctccgaa
     1921 gtgcgtcgtc tggatgtagc gctcttcttc cacgcaatac gcggtcaatc tgccccagga
     1981 caccagcgcg gccgcggtgt ccagcagcac gatttgcggg ccgccgatcg gcgtcatcgg
     2041 ggctttgcgc gggtcctggt agtacgaatg caccggcgtg tggccgaaga agccgcgctt
     2101 gccccagttc ttttcgcctt ggattccttc caactggaat cggccccata gcacgtcctg
     2161 gcgcatgtcg ggctcgacgc gcagatattc ggaaatcgtc ggcgggtagc acgtcgtcgt
     2221 cggcgaccat cgcccgtgca ggacgaagaa tttctcgtgc tcgaacaccg gcggcagctc
     2281 gtggatgaag cgcttgtgat cgtccggcac gaactgcgtc agctgccgca tgcggtccgg
     2341 cgtcgggctt cttgccgttt cgttcagcca atgccagtcg gcgccgtagc tgtagaacgt
     2401 cgagtccatg ccataatcca tgaaccactg aaatgcgatg accggattgg cgctggcggc
     2461 ggattcggcg tagctgtggc cgtgcagcac caggtcgaag acgtcgtcat ggttgccgcg
     2521 gatgaagtgg gcgcgatcga gcgtgagcag caaatcgatc acttttgaag aatccgggcc
     2581 gcggttgacg tagtcaccgg cgaagatcag acgagcggcg gggtcgtcgg cgttaaccgc
     2641 gtccagcaac cgccgaaggg ggcggagcat tccgtgtacg tcgccgatga tccagtgcat
     2701 gggtgtttga taccttaccg gcgaagatga actgcggcta gaatcaacgg gatcatcccc
     2761 aacggaaggt gctatgcccg ctcgaaaaac cgtttatccc aaaaacccga tcgccgacga
     2821 actgttgaag aagtacagca aaccaatgaa gcctggctcg ggcgcgctga gtgcgaagga
     2881 tgtcgccaat cgattgaagg gcctcaacgg ctggaccgct gccgacgggc ggatcagcaa
     2941 gacgtacaag ttcgacgatt actatcgcac gatcaccttc gtgaacgcgg tagcatggct
     3001 ggcgaacaag gaagaccacc acccggacct ggaggtgggg tacaaccagt gtgtggtgca
     3061 ttacagcacg cactcggtgg ggggactgtc ggagaacgat ttcatctgcg ccgcgaaggt
     3121 ggatgcgctg ctgagactgt aaacgcgcca ttgtctcgag caccgcgtgg tttagccgcc
     3181 gcggcctcgc ggcgcgagtg gtgatacgcg cggttagcgc aagagcaccg cgaggccgcg
     3241 gcggctaaac tgtatggttc ttgggaaatc cggtggccac ggcgcaatcc gcgcccgcgt
     3301 cgcgcggttc cgcaattctt gacacccccc gtgcacacct atacaagtga tttcgctggt
     3361 tcgggttgga ggcgactggg gggtttgccc gacgccagca actatgtcct tcgggattcc
     3421 catcctggct gagacgtcgg tttcgcccat cctggtcgaa aaactgatct tcggcgacga
     3481 cccgagcttc ctctggcatg tcatctacga cctggcgatc gtcgcggtcg tctttctgcc
     3541 gctgatctcc gtcattgcaa tgatcagcat ctgggccgaa cgccgagtgg cggggcggat
     3601 gcagagccgg ctgggaccga atcgagtcgg gccgtttggt ttacttcaat cggtcgccga
     3661 tggtttgaag ctgctgctca aggaagacct gctgccggac gaggcggacg ggtttctgtt
     3721 tcacgtcgcc ccgtatctcg cgttcgcgcc ggttttcgcg gcttacttgg cgctggcgtt
     3781 cgggccgaac ctgacgttcg agccgcgcct caacgtcggc gtcttctggc tgctggcgat
     3841 cctcagcgtc gagatcatgg gcgtgatcct ggccggctgg gcgagcaata ataaatgggc
     3901 catctacggc gcgatgcgcg aggcgtgcca gatggtcagc tacgaaattc cgctcggcat
     3961 ctcgatcatc atcggcgtga tgacggccgg cacgctgaac ctggtgcagc tgggccacct
     4021 gcaaagcggc gggatccaca cgtggatcat ctttcgcaac ccgttcgtct tcgcggcgtt
     4081 ctttctttat ttcgtcgcca gcctcgcttc caacaaacgc gcacccttcg atctgcccga
     4141 atcggaatcg gaactggtcg ccgggtttca caccgaatat tccgggctgc ggttttcgtt
     4201 tttcttcttc gccgaatacg ccgccatgtt cgtcgtcgcc ggcatccaga ccggcttatt
     4261 cctcggcggg tggaacgatc cgttcgggct gctcggctac tactaccacc gcttcaccgc
     4321 tgatccggaa aacgtcaaca ccgccgggtt aatcgcggtg aacctggtcg gctgcgcgat
     4381 cttcatgacc aagtgcatca cgatcatctt cgtgcagatg tggatccgct ggacgttccc
     4441 gcggccgcgg atcgaccagg tgctgtacgc gtgcgtgaag gtgatgctgc cgctggcgtg
     4501 cctgctgctg ctgggggcga cgctgtggca gctgttcgtg ccggaaatct ccggcatccc
     4561 gtggtcgaac aacacaaatc cgtggattcc gccgcagcac gagatcttcg cctggctggt
     4621 gcaggtgctg ctggcgctga tcggcgtcgc cgggttcagc gcggtggtcg gctgggtggc
     4681 ctacgccttc gccagtggcc gcggattaaa gcagcggttg acggacccgg ctccgatcgt
     4741 gaccgaagcg gatgcgctgg tgacccaaac gtagcatggg cgtctcgccc atgccttgat
     4801 gctccgacac gggcgagacg cccgtgctgc atgaaagcga tcctacccat cctcgccacc
     4861 accgacgccg acatggcggg cgcggtgttg ttctacctct ttgcgctgat ggccggtggg
     4921 agcgcgatcg gcgtggtcat ctcgcgcaac atcgtccgca ccgcggtctt tttgctgttc
     4981 acattgaccg gcgtcgcagg gttgtacttc ctgctgaacg ccgaattcct tgccgcggtg
     5041 caattggttg tatacgcggg tggcacttta atcctgatcg tgttcggcgt gatgctgacg
     5101 agcaaatcgc cgttcagccg gttcgagccg aagctggtcg aggtgatcgt cgcgctgtcg
     5161 atcgcggtga tcctgctgac ggcgttgatc atggcgatcc accgcacgcc gttcccgcag
     5221 tcggcggacg tcgcgacgga tggatacccg atggcccagc tggggcaggc gttgctgggc
     5281 gattatctcg tgccatttga gttggcgagc gtgctgttgt tggtggtgat gatcggcgct
     5341 gcgtacctgg ccaaggggcg cagacgggaa gaaggcacgc cggcacaatc gcagtcccgg
     5401 ttgtcttcgc aggcgcggca atggagctga gggtcattca cgcaagtgct gcgcgagggg
     5461 acccaaacga gaagcatgaa cgtgctcgcc caatccaact tcgccaccat cggcctgagt
     5521 cattacctgc cgctggccgc ggtgatgttt gtttgcgggc tgctgtcgat cctgctgaag
     5581 cgcaatgcga ttggcatcct gatgggcgtc gagctgattc tgaacgcggc caacgtgaac
     5641 ctcgtcgcgt tcaaccgatt cagctacgcc gggactagcg gctcaccgct gttgaccggg
     5701 cagatattcg ccgcattcgt cattgttctc gccgccgccg aagccgcggt ggcgctggcg
     5761 atctttttga acttctataa caacttcagc acgattgatg tggagcgggc gcagaatctt
     5821 cgcgggtgat tcatcacccc ttgtcctgag cggaggcggt gctccgccgg agtcgaagga
     5881 tattccaacg agtggtggtg aacatttact ggcgcgaaaa gacactgatt gagacggaat
     5941 gaactgaact gacgaagccg agcttcaccg acggtcgagg ctatatcctt cgacttcggc
     6001 ggagtaccga ctgcgctcag gacaaggcgt aagacatgcc gactcccgca acgcttctaa
     6061 caatcgccac gctcctgccg cttgcggcat tcgtgctgct cgtgttcgtc ggcaagcgca
     6121 tgggtacacc gctggccggt tacgtcggca ccgcgttcat aggggcagcc ttcgtctgca
     6181 gcatcatcgc gatgatcagc tggtacgacg gcgggatgta cgccggccgg cagtgggggt
     6241 ttgagaaggg gccgatcaac atcccgatcc agtggatccc gatcggcacg ccggacaagc
     6301 cgagcggcat cgatcaggac cacccgggat ttttggacct gggcatcttc gtcgacagcc
     6361 tgaccatcac aatgttcgcg atgatcacgc tcgtctcgac gctcgtgttc atcttctcaa
     6421 tcggctacat ggccgaggac aaacgcttcc cgcggttctt cacgtacctg ggcctgttct
     6481 gcttttccat gctcggcctg gtcatcggcg gaacgacgct tcagctgttc atcttctggg
     6541 aactggtcgg cctgtgctcg tacctcttga tcggcttctg gtacgaaaag aagtccgcca
     6601 gcaacgccgc catcaaggcg ttcatcgtca accgcgtggg cgacttcggt tttctgatcg
     6661 gcttcggcat cctgttctat cacattggca acgtgacgct gccgcacatg tgggtgtcgc
     6721 tgggcaacgc cgggtcaggt ttgccgatca cgctctccag cggcgttgtc ttcagcagcg
     6781 gattgctgag cgtgatgggc atcgggctgt tcttcggggc ggtcggcaag agcgcgcagt
     6841 tcccgctgca cgtctggctg ccggatgcga tggaaggccc gacgccggtc agcgcgctga
     6901 tccacgccgc caccatggtg gccgcgggtg tgtacctggt tggccgaatt ttcccgctcc
     6961 tcacacccga tgcaaaactg ttcatcgcga ttatcggctg cacgacgctg acgatggcgg
     7021 cgctgatcgc catcgcgcag acggacatca agaaagtgct ggcgtacagc acgctgtcgc
     7081 agctgggtta catgatcctg gcgatgggcg tgggcagctg ggtcggcggg ctgttccacc
     7141 tgatcaccca cgcgttcttc aaggcgctgc tgttcctggg cagcggcagc gtgatccacg
     7201 cggcgcatca cgagcaggag atgccgaagt acggcgggct cattcgaaag attcccgtca
     7261 ccggcatcac gttcggaatc gccgtcctgg cgatctccgg cgtcggcttc gggcaattcg
     7321 gcttctccgg attttacagc aaagacatga tcctcgccca cgcgggagcg ttcgcgacgc
     7381 tcgcgcacgt ggtgggcaag tccaaggcgt attacctgct gttcgcgctg ccgaccatca
     7441 tcgccttcgt cacgccgttc tacatgacgc gctgctggat gatgacgttc gccggcaagc
     7501 cgcgcgatga gcatctgtac gaacacgcgc atgagtcgcc catcatgtgg ggaccgctgg
     7561 tggtgctggc tatcctcgcc gtcatcagcg gaaagtggat gagcattcag gagatgatcg
     7621 aaggatcgat tcaggagaac accgcgtatt gccgggggat cgatccaagc ttccacggct
     7681 ttgggacagc ctggccaagc cgggtggcgg agaacatcgg cttggaagtg cgcgacattg
     7741 ccgaggtgcc accgggcaca cccagcgtgc cgcacggggc gcccccggcg gtggcgatga
     7801 ccgaagcgca gcacgcgcac gtcattggac atgagatggt cgagaagtgg ctgggcttct
     7861 ggggattcac cagcggcatc ttggcgggtg tgattgtcta ctggcgcggg ttgggcgtcg
     7921 cgcaagccgt gctgaagttc ccgccggcgc ggtgggtgcg caactggctc tacggccgga
     7981 tgtacttcga cgagttgtac ttctacgtct tcgtcaattt cgtgatggcg atgtcctggc
     8041 tggctggttg gttcgaccgc attgtggttg acggcatcgt caacggcgcc gcccgcatgg
     8101 tgcgcgagat ggcgatgctc gcggggctga acgaccgtta cgttgtcgat ggcaccgtga
     8161 acggcgtggg cgagttggcc cacagcctcg gcgccgccgt gcgagcgccg gcgacggcgg
     8221 gtcgcgtgc
//
LOCUS       DATIMH010000648         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1024642, whole genome shotgun sequence.
ACCESSION   DATIMH010000648 DATIMH010000000
VERSION     DATIMH010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024642"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VLI90_11230"
     CDS             <1..222
                     /locus_tag="VLI90_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14825.1"
                     /translation="PWLLYTRDVNGRGLGFVTAHRLPLGYGGVIELPGPLGEPLTIHC
                     TLLRCREAAPGWFEGSVYFNREQPKFAAE"
     gene            324..1274
                     /locus_tag="VLI90_11235"
     CDS             324..1274
                     /locus_tag="VLI90_11235"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HSV14826.1"
                     /translation="MRARLCFLLPLLCIAAIAPAAPTTAPVGKLPHIEVDVQQKQVHV
                     ECEAVRADYPLEFLAVVRNGNEYESIVRSDAKPSDVHLALLMLGLKPGEPVHYSESTK
                     TWLPPSGPPIVIWFEYDKDGKHEKIPATRWMRDVRSKREPPAFSWVFTGSKTMADGTY
                     AADPTGYLISVINNELSVLDVPDLKARALEARELERNPDTMPPTGTPVTMILSPAGAD
                     NAAPPPQQATSPATTQASLSDVHIDQQKVDRLRERWQQAVAPHRGALKEAAQAHYEVI
                     NELRREQQRLIDEADRIQRTIDQLEKEYQDMTTPRPEAAP"
     gene            1462..>2080
                     /locus_tag="VLI90_11240"
     CDS             1462..>2080
                     /locus_tag="VLI90_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14827.1"
                     /translation="MKARESLSRSLVPLALGAAASVACYAAAGATLNLPLGGVLFAAI
                     LAPTLASDQTRLNLITSVAFTLGVAAAWLALPSGWLGATLVLLSFTAAAVFATQSLIR
                     LRIHSIAAAAIVVTIALAWLTWPVWASGFIAGKTIGWPVALHPLFAINAACRDLGMWT
                     EQRVAYRLTNLGQDATYQLPTGALPCIAFQTALAVLFYLVARAQHR"
BASE COUNT          354 a          721 c          653 g          352 t
ORIGIN      
        1 ccgtggttgc tctacacgcg cgacgtcaac ggccgcggcc tcgggttcgt caccgcccac
       61 cgcctccccc tgggctacgg cggcgtgatc gaactccccg gcccgctcgg cgagccgctg
      121 acgatccact gcacgctgct ccgctgccgc gaagccgcgc ccggctggtt cgaaggcagc
      181 gtgtatttca accgcgagca accgaagttc gcggccgagt gattgtagct gcgacgcctg
      241 tctcgcctct tcatgcaagg gcgaagcagg cgtcgcagct acctgatctc gccccgtcgc
      301 tcgcgcgccg ctagacttct gtcatgcgcg ctcgcctctg ttttctcctt ccgctgctct
      361 gcatcgccgc gatcgccccc gccgccccga ccaccgcgcc agtcggcaag ctgccgcaca
      421 tcgaggtgga cgttcagcaa aaacaagtgc acgtcgagtg tgaagccgtg cgcgccgact
      481 acccgctgga gttcctcgcc gtcgttcgca acggcaacga gtacgaatcg atcgtccgct
      541 ccgacgccaa gccatcggat gtccacctgg cgctcttgat gctcggcctc aagcccggcg
      601 aaccggtgca ttactcggag tccaccaaaa cctggctccc gccgagcgga ccgccgatcg
      661 tcatctggtt cgaatacgac aaggacggca agcacgagaa gattcccgcg acgcgctgga
      721 tgcgcgatgt gcgcagcaag cgcgagccgc cggcgttcag ctgggtgttc accggatcca
      781 agacaatggc cgacggcacg tacgccgccg atccgaccgg gtatctgatc agcgtgatca
      841 ataatgagct gtcggtgctg gacgtgccgg acctgaaagc gcgggcgctg gaagcgcgcg
      901 agctggaacg caatcccgac accatgccgc cgaccggaac gccggtgaca atgatcctgt
      961 cccccgccgg ggccgacaac gcggcgccac cgccgcagca agcgacctcc ccggcgacga
     1021 cgcaggcgtc gctgtcggac gtgcacatcg atcagcagaa agtcgatcgg ctgcgcgagc
     1081 gttggcagca ggccgtcgcc ccgcaccgcg gggcgctgaa ggaagcggcg caggcgcact
     1141 acgaagtgat caacgagctc cgccgtgagc agcagcggct gatcgatgaa gccgaccgca
     1201 ttcagcgtac gatcgatcag ctggaaaaag agtatcagga catgaccacc ccgcggccgg
     1261 aggcggcgcc ataaacgccc acgcgcgcgc gaagagattt tcaagcgtcg aacaccccat
     1321 ttagccccgc gtgaacacac gccgggttta cccgggtcgt cgcggccttc tgttgagaaa
     1381 acgaacgcgc ctcgacttct caacgaaacc gggcgtgtgt tcacgccggg ctaaatgggc
     1441 gattcgcgcc acccacgcac gatgaaggct cgggagtcac tttcgcggtc gctcgtcccg
     1501 cttgcactgg gcgcggcggc gtccgtcgcg tgctacgccg ccgccggcgc gacgctgaat
     1561 ctaccgcttg gcggtgtgct cttcgccgcg atcctcgcgc cgacgctcgc gtcggatcag
     1621 acgcgtttga acttgatcac cagtgtcgcc ttcacgctcg gcgtcgccgc cgcgtggctc
     1681 gcccttccgt caggctggtt aggggcaacg ctcgtgctgc tgagcttcac ggctgccgcg
     1741 gtcttcgcaa cgcaatcttt gatccgattg cgcatccact ccatcgccgc cgcggcgatc
     1801 gttgtgacga tcgcgctggc gtggctgacg tggcccgtgt gggcttcagg gttcatcgcg
     1861 ggaaagacga tcgggtggcc cgtggcgctg catccgctgt tcgccatcaa cgccgcgtgc
     1921 cgcgacctgg gcatgtggac cgagcagcgc gttgcgtatc gattgacgaa tctcgggcaa
     1981 gacgcgacgt atcagctgcc aacgggcgcg ctgccgtgca tcgcgttcca aacagcgctt
     2041 gcggtgctgt tttatttagt ggcgcgtgcg cagcaccgcg
//
LOCUS       DATIMH010000649         1839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4781860, whole genome shotgun sequence.
ACCESSION   DATIMH010000649 DATIMH010000000
VERSION     DATIMH010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4781860"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /gene="greB"
                     /locus_tag="VLI90_11245"
     CDS             <1..483
                     /gene="greB"
                     /locus_tag="VLI90_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HSV14828.1"
                     /translation="PPPRFITPQGLVRIRAEYDKLFSAERPKLVETIAWAAANGDRSE
                     NGDYIYGRKKLREIDRRLGYLAKVMKAAKVVDPATQEARDQVRFGATVELADEDDVRR
                     TVTIVGDDEADASAGKIGWSAPLARSLIGAKAGDERVVRLPAGEKSYEILAVRYPDPN
                     "
     gene            complement(480..1184)
                     /locus_tag="VLI90_11250"
     CDS             complement(480..1184)
                     /locus_tag="VLI90_11250"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14829.1"
                     /translation="MVNAGQTATLAALRDHLGELQQAQIAHGRRAIILLEGPPGSGKK
                     AALRQLAAAFDPCYLAVHQCSFDRREASRGHWLAGFWAQLPSAGNTAVFFRSWYRRVL
                     DDRVLGRVDETSIPRTFDEINEFEAQQRDYGTLIVKLYFSAADTVQRQRIDACWTRQW
                     PQLRREEPVSVSDSAYARAFAELQANSDTRWSPWKLIDANDEGQGSIAALEAIADAWS
                     KAMPANPPQLIQTIRA"
     gene            complement(1276..>1839)
                     /locus_tag="VLI90_11255"
     CDS             complement(1276..>1839)
                     /locus_tag="VLI90_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HSV14830.1"
                     /translation="AAKLAGAARTRVGEQLGLIEEGSFRFCWIVDFPMYEYDEERQKI
                     DFSHNPFSMPQGEMEALETKDPLDILAWQYDIVCNGVELSSGAIRNHRPDIMYKAFEI
                     AGYTKEEVDTNFAGMINAFKFGAPPHGGSAPGIDRIVMLLADEPNIREVILFPMNQKA
                     EDLMMNAPSEVMEKQLKELHIKLVPQG"
BASE COUNT          335 a          588 c          571 g          345 t
ORIGIN      
        1 ccgccgccgc gcttcattac gccgcaaggc ctcgttcgaa tccgcgccga atatgacaag
       61 ctgttttcgg ccgagcgccc caagctggtg gaaacgatcg cctgggccgc cgccaacggc
      121 gaccgttccg aaaacggcga ctacatctac ggtcgcaaga agctgcgcga gatcgaccgt
      181 cgcctcggct acctcgccaa ggtgatgaaa gctgccaagg tcgtcgatcc cgccactcag
      241 gaagcccgcg accaggtgcg gtttggggca accgtcgaac tcgccgacga ggatgatgtc
      301 cgccggaccg tgacgatcgt gggagacgat gaggcggatg cgtcagccgg caagatcggc
      361 tggagcgctc ccctagcccg ctcgctcatt ggcgcgaagg ctggcgacga acgtgtcgtg
      421 cgcctgcccg ccggcgaaaa gagctacgaa atcctggcag tccggtaccc ggacccgaac
      481 taggcgcgga tcgtctggat cagctgcggc gggttggccg gcatcgcctt ggaccaggcg
      541 tcggcaatcg cttccagcgc cgcaatactg ccctgccctt catcattggc gtcgatcagc
      601 ttccacggtg accagcgcgt gtcgctgttc gcctgcaatt cggcaaaggc gcgagcgtaa
      661 gcagaatcgg aaaccgatac cggctcttcg cggcgaagct gcggccattg ccgcgtccag
      721 caagcatcaa tccgctggcg ctgcacggtg tcggcggccg agaaatagag cttcacgatc
      781 aacgtcccgt aatcgcgctg ctgcgcttca aactcattga tctcatcgaa agtacgcggg
      841 atgctggtct cgtcgacccg gccgagaacc cggtcatcga gtacgcgccg ataccaactg
      901 cggaagaaga ctgccgtgtt gcccgcgctt ggaagctgcg cccaaaagcc cgcaagccag
      961 tggccacgcg acgcttcgcg gcggtcgaag ctgcattggt ggaccgccag gtaacaagga
     1021 tcgaaggcag ccgcgagctg ccgcaatgcg gctttcttgc cggagcccgg cggcccttcg
     1081 agcaggatga tcgcccgccg gccatgcgcg atctgcgcct gctgcagttc acccaggtga
     1141 tcgcgcagcg cggcaagcgt cgcagtttgt ccagcgttga ccatattccc cacagcgcga
     1201 atttagcacg cggagcggtc ccggcaatca tccagcgggc ccaaacccat gatttcgtcc
     1261 cgttttgggc cgcgcttagc cctgcgggac gagcttgatg tgaagctcct tgagctgctt
     1321 ctccatcact tctgatggcg cgttcatcat caggtcctcg gccttctggt tcatcgggaa
     1381 gaggataact tcgcgaatgt tcggttcatc agccagcagc atgacgatac ggtcgatgcc
     1441 cggggccgag ccgccgtgtg gcggggcgcc gaacttgaag gcgttgatca tgcccgcgaa
     1501 gttcgtgtcg acttcttcct tggtgtaccc ggcgatttcg aacgccttgt acatgatgtc
     1561 cggacggtgg ttgcggatcg cgccgctgct cagctccacg ccgttgcaaa cgatgtcata
     1621 ctgccacgcg aggatatcga gcgggtcctt cgtctccagc gcctccatct cgccctgcgg
     1681 catggagaag gggttgtggc tgaagtcgat tttctgccgt tcctcgtcat attcgtacat
     1741 cgggaagtcg acgatccagc agaagcggaa tgagccttcc tcgatgaggc cgagttgttc
     1801 gccgacgcgc gtccgcgcag cgcctgcgag cttggcggc
//
LOCUS       DATIMH010000650         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1317319, whole genome shotgun sequence.
ACCESSION   DATIMH010000650 DATIMH010000000
VERSION     DATIMH010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317319"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..2700)
                     /locus_tag="VLI90_11260"
     CDS             complement(34..2700)
                     /locus_tag="VLI90_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HSV14831.1"
                     /translation="MTLAERKAARGRLKVFFGAAAGVGKTYAMLEEGRARAGDGLDVV
                     VGYAEPHGRRETELLLLGMELLPCKTVEYKGATLRELDLDAVLKRRPQLALVDELAHT
                     NAPDLRHAKRWQDVVELLDAGINVYTTLNVQHLESVNDIVERITGVRVRETLPDSVLE
                     QADEVELIDTSPEELLERLQEGRIYAGEQAHRATKHFFTKGNLTALRELALRRTAERV
                     DAQMQDLRRHQGTHDAWAATERILVCVGPSPLSARLVRSAKRLADGLKAAWIAAYVET
                     PATMSQADRDRVAQTLRLAEQLGGQSVTLSGANPSDELIAYARARNVTKIVIGKPDRP
                     RWKELLLRRSIVDDLIRRSGDIDVYAIRGEPTGPAVEQSNASAPPWNLWPFGWAALTV
                     VVCTLIDLGMYRALGYGAQSDLSNLVMVFLLGVVLTAVRFGRGPAVLASLLSTAALDF
                     LFVPPFYTFAVADTQYFITFGVMLAVAMIVTTLTDRVRGQAELSRRRESRTAALLDLS
                     RELAATRDAEAIAKAATGQLAEVFDAEVMILVAAGDGALQVRVARVDTFSLDKKELSV
                     AQWALTHDQIAGVGTATLPSARGLYLPLIASRGTVGVLAVRPSDKSRLQDPEQLRLLQ
                     AFANLIAATMDRANLADEARAAWERVEAEFLRNTLLSSVSHDLRTPLAAISGAAGTLL
                     QANGSIDDATRRDLAETVLDESRRMERVISNLLDMTRLESGGLQLKRQWQPLAEVVGA
                     ALHEMDQRLAGRQVRVDLPADLPLVKIDDVSMEQVLVNLLDNAVEYASAGGAIEIVGR
                     GGDGKVTLEIADHGPGIPPGTEQQIFQKFFRSHPQGGRRGIGLGLAICRGIVEAHGGT
                     INATNRPGGGAVFRIELPVEGAPPPPVEKAEE"
     gene            complement(2736..3332)
                     /gene="kdpC"
                     /locus_tag="VLI90_11265"
     CDS             complement(2736..3332)
                     /gene="kdpC"
                     /locus_tag="VLI90_11265"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HSV14832.1"
                     /translation="MLKQLRASIVILLLLTAITGVAYPFAITVIAKTCFPRQAAGSLL
                     ANGAGSKLIGQPFDDPRYFWGRPSATVDAAGKPLPYNAAGSGGSNLSPTNPQQIDLVK
                     SRVEALQKSEPGNTAAIPVDLVTASASGLDPHITPAAADYQVHRIAVARHLPEQAVRD
                     LVRQHTESRQLGVLGEPRVNVLELNIAIDSLQASSNPS"
BASE COUNT          568 a         1218 c         1031 g          586 t
ORIGIN      
        1 ttttatctgt gttcatctat gtgcatctgt ggttcattct tccgccttct ccaccggcgg
       61 cgggggcgcg ccttcgaccg gcaattcgat gcgaaacacc gccccaccgc cggggcgatt
      121 cgttgcgtta atcgtcccgc cgtgtgcttc gacgatgccg cggcagatcg ccaaacccaa
      181 gccgatcccg cggcggccgc cttgcgggtg cgagcggaaa aacttctgga aaatctgctg
      241 ctcggtcccc ggcggaatgc cggggccgtg gtcggcaatc tccagagtca ccttgccatc
      301 gccgccgcga ccgacgatct cgatcgcccc gccggccgac gcatattcga ccgcgttgtc
      361 cagcaggttg accagcacct gctccatcga gacatcatcg atcttcacca gcggcaggtc
      421 tgccggcaaa tccacccgca cctgccggcc ggccagtcgc tggtccatct cgtgcaacgc
      481 cgcccccacc acttccgcca gcggttgcca ttgccgcttg agttgcagcc cgccggattc
      541 cagccgcgtc atgtcgagca ggttcgagat gacgcgctcc atccgccgcg attcatcgag
      601 caccgtctcg gccaaatcgc gccgcgtcgc gtcgtcgatc gatccatttg cttggagcaa
      661 cgtgccggcc gcgcccgaga tcgcggcgag cggtgtgcgg agatcgtgcg aaacgctgct
      721 cagcagcgtg ttgcgcaaaa actccgcctc cacgcgttcc cacgcggccc gcgcttcatc
      781 agccaaattc gcccgatcca tcgtcgccgc aatcaagttc gcgaacgcct gtagcaagcg
      841 aagttgctcg ggatcctgca gccggctctt gtccgacggc cgcaccgcca gcacaccgac
      901 ggtgccgcgc gacgcgatca gcggcaggta cagcccgcgc gccgacggca gcgtcgccgt
      961 gcctacgccc gctatctggt cgtgcgtcaa tgcccactgc gcgacgctca gttccttctt
     1021 gtccagcgaa aaggtgtcca cccgcgcgac gcgaacctgc agcgccccgt cacccgcggc
     1081 aacgaggatc atcacctccg catcgaacac ctcggccaac tgccccgtgg cggctttggc
     1141 gatcgcctcg gcgtctcgag tcgccgccag ctcccggctc aggtccaaca gcgcggcggt
     1201 gcgactttca cgtcggcgcg acaactccgc ctgtccgcgc acgcggtcgg tcagcgtcgt
     1261 cacgatcatc gccaccgcca gcatcacgcc gaacgtgatg aaatactgcg tgtcggccac
     1321 agcgaacgtg tagaacggcg ggacgaacaa aaaatccagc gccgcggtgc tcagcagcga
     1381 cgccaacacc gctggcccgc ggccgaagcg cacggccgtc agcacgacgc cgagcaggaa
     1441 gaccatcacc aggttcgaca gatccgactg cgcgccgtac ccgagcgcgc gatacatccc
     1501 gagatcgatc agcgtgcaga caacgacggt cagcgccgcc cacccgaacg gccacaaatt
     1561 ccacggcggc gccgaggcgt tcgattgttc aaccgccgga cccgtcggtt cgccgcggat
     1621 cgcatacacg tcaatgtcac cgctgcggcg gatcaggtca tccacgatcg atcgccgcag
     1681 cagcagctcc ttccaccgcg ggcgatccgg cttgccgatg acgatcttcg tcacgttgcg
     1741 cgcccgcgcg taggcgatca attcgtcgct gggattggcg ccgctgagcg tgacgctctg
     1801 cccgccgagt tgctcggcca gccgaagcgt ctgcgcgacg cgatcgcgat ccgcctgcga
     1861 catcgttgcc ggcgtctcga catacgccgc gatccacgcc gctttcaatc catccgccag
     1921 gcgcttggcc gatcgcacca agcgcgccga taatggactg ggaccgacgc agacgaggat
     1981 gcgctccgtc gccgcccagg catcgtgcgt gccttgatgc cggcgaaggt cctgcatctg
     2041 cgcatcgacg cgctcggcgg tccgccgcag cgccagctcg cgcagcgccg tcagattgcc
     2101 cttggtgaag aaatgcttgg tcgcgcgatg ggcctgctcg ccggcgtaga tccggccttc
     2161 ctgcaggcgc tcgagcaact cctcgggcga ggtgtcgatc agctcaactt catccgcctg
     2221 ctccagcacc gaatccggca gcgtctcccg cacgcgcacg ccggtgatcc gctccacgat
     2281 gtcgttgacg ctttccaggt gctgaacgtt caacgtcgtg tagacgttga tgccggcatc
     2341 gagcagctca acgacatcct gccagcgctt ggcgtggcga aggtccggcg cgttggtgtg
     2401 cgccagctcg tcgaccagcg ccagttgcgg ccttcgtttc aacaccgcgt ccaagtccag
     2461 ctcgcgcagc gtcgcgcctt tgtattcgac ggtcttgcac ggcaggagtt ccatgccgag
     2521 cagcagcagc tcggtttcgc gccgcccgtg cggctcggca tatccgacga cgacatccaa
     2581 gccatccccc gcccgcgccc ggccctcctc cagcatcgca tacgtcttac ccacccccgc
     2641 ggcggcgccg aagaacacct tgagccgccc gcgcgcggcc ttccgctccg cgagcgtcac
     2701 cgcctgcaac agcgaatcgg gattgggtcg ttcggttacg atgggttgct cgatgcttgc
     2761 agcgaatcaa tcgcgatatt cagttccagc acgttcaccc gcggctcgcc gagcacaccg
     2821 agctgcctcg actcggtgtg ttgccgcacc aggtcacgca ccgcttgctc cggcagatgc
     2881 cgggccaccg cgatgcgatg cacctggtaa tccgccgccg cgggggtgat gtgcggatca
     2941 agcccgctgg cggaggcggt gaccaaatcg accggaatcg cggccgtgtt gcccggctcg
     3001 gacttttgca acgcctcgac ccgcgatttt accagatcga tctgctgcgg gttggtgggc
     3061 gacagatttg acccgccgga cccggcggca ttgtacggca gcggcttgcc cgcggcgtca
     3121 acagtggccg agggacgacc ccagaagtaa cgcgggtcgt cgaagggctg cccgatcagc
     3181 ttcgatccgg cgccgttcgc taagaggctt cccgccgcct ggcgcgggaa gcaggtcttg
     3241 gcgatgaccg tgatggcgaa cggatacgcc acgccggtaa tggcggtgag caggagaaga
     3301 atgacgattg atgcgcgcag ttgtttgagc atgtgaggtc ctcggtcacg ctgacggctt
     3361 ctagaaactc ctgtgcgttg tagccgcgac gcctgcgtcg cct
//
LOCUS       DATIMH010000651         3745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5359832, whole genome shotgun sequence.
ACCESSION   DATIMH010000651 DATIMH010000000
VERSION     DATIMH010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5359832"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..689)
                     /locus_tag="VLI90_11270"
     CDS             complement(72..689)
                     /locus_tag="VLI90_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14833.1"
                     /translation="MPSLNLSRPLAVVVTLLLLSGATPTARANFVLLSHHLTVDRTAD
                     TASFDLVFNQKPDLHSVDSDGRAINSFQIEFAGDHALDGLPYPQNLTAVIRGEEIHIA
                     DAVRIRAPIGDGGPNSGGWGPVIDSEPFTVDQNKVSFSTSIEDLGLRGRTFNYSVYSL
                     ESGSLTAIEEVHVVPLPPAVEMFVPAGLILMIAARLHVRAEKRAK"
     gene            845..1843
                     /gene="galE"
                     /locus_tag="VLI90_11275"
     CDS             845..1843
                     /gene="galE"
                     /locus_tag="VLI90_11275"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HSV14834.1"
                     /translation="MNVLVAGGAGYIGSHTVKRLAAAGHSPVIYDNLSRGHKTVADIL
                     KVPFVAADLNDRTTLTTTLRENKIEAVMHFAAYAYVGESVDKPLMYYQNNVATTISVL
                     QSMQEVGIDRFVFSSTCATYGDPDKIPITEDEKKAPVSPYGRSKWMVEQILQDTPGGW
                     KDFKFAALRYFNASGCAMDGSLGEDHDPETHLIPVVLQAIMGVRPGITLFGTDYPTKD
                     GTNVRDYIHVDDLADAHIAAMERLDEQKKIVANLGTGHGFSVKEIIATAEKVTGKKVP
                     VTYGPRRPGDAIALYADPTHAKRSLGWEAKYKDPETIIRSAWNWFSKHPNGYGARK"
     gene            1928..2812
                     /locus_tag="VLI90_11280"
     CDS             1928..2812
                     /locus_tag="VLI90_11280"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HSV14835.1"
                     /translation="MSENQTMLLRLGHSPDPDDAFMFYGLAKDKLPTGPYRFEHVLQD
                     IQTLNRRAMNGELEITAISIHAYPYVADKYALTSCGSSMGDKYGPMVIAREGMPLEAL
                     KGKTIAIPGDLTTAFLTLQLCLGKGAKRGSRQRGAFEYDVVHFDEIPKYIRDGKADAG
                     LIIHEGQLTYQTLGLQLLVDLGMWWHQRTALPLPLGGNCIRKDLGPRVMQEVTDILKR
                     SIQYSLDHRAEAVEYALQFGRDLDRKLADQFVGMYVNDWTLDYGPRGREAITRLLKEG
                     AAAGLVPNVGDVQYVTAK"
     gene            2925..>3745
                     /locus_tag="VLI90_11285"
     CDS             2925..>3745
                     /locus_tag="VLI90_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HSV14836.1"
                     /translation="MVIRRTAVFFALFLSLLASSASPAFAQPAAATARQTRDPLHVLL
                     ITMGPGADPWEKFGHNAIEIVDLETGSDRCYNWGVFSFGEGVSGFLGFTWHFLQGRLW
                     YSMQSDPAGPMLEAYADAGRSVLVQELQMTHEQKAALQHRLQAQDTDANRYYLYDYFL
                     KNCSTMARDAIDQTVDGRVAAALRDVPTGTTYRWHDRRLTADELWLYTFLDFALGHPI
                     DHELSEWQESFLPMKLATHLREVRVPDPDGHLVPLVMWEKQLAPGAFQERDTPPR"
BASE COUNT          734 a         1190 c         1174 g          647 t
ORIGIN      
        1 ccggcgtacc gggcgagggg accggaacaa atacccggcg ctggttgtgt gaagatcgat
       61 cgcggccgcg ttcacttggc gcgtttttcg gctcgaacgt ggagacgtgc ggcaatcatt
      121 aggatcaaac ccgccggaac gaacatctcc accgccggcg gcagcgggac gacgtgcact
      181 tcctcgatcg ccgtcagtga gcccgactcc agcgaataga cgctgtaatt gaacgtccgc
      241 ccgcgcagcc ccaggtcctc aatgctcgtc gagaagctga ctttgttctg atctacggta
      301 aacggctcgc tgtcgatcac gggcccccac ccgccgctgt tcggcccacc gtcgccgatc
      361 ggggcgcgga tgcgcacggc gtcagcgata tggatctcct cgccgcggat caccgccgtc
      421 aggttctgcg ggtagggcag gccgtccagc gcgtgatcgc cggcgaattc gatttggaac
      481 gagttgatcg cccggccgtc gctgtcgacg ctgtgcaagt ccggcttctg gttgaagacg
      541 agatcgaacg atgcggtgtc cgccgtgcga tcgacggtca aatgatggga gagcaggacg
      601 aaattcgccc gcgcggttgg tgtagcgccc gacagcagta acagcgttac caccacagcg
      661 agcgggcgcg acagattgag agatggcata cgtcagccgg ccaacggcgc cggccgagcg
      721 cgaacatacc gcgcggcatc cttagcgaga acggaccgaa tgccacggaa tccgcccgta
      781 ccatttgccc cgcggcgtcc gtatactgcc gcgctctttg gcttgagcag aaggagtcca
      841 agcgatgaac gttctggtcg ccggcggggc cggttacatc gggtcacaca ctgtgaagcg
      901 gttggccgcc gcggggcatt cgccggtcat ttatgacaac ctctcgcgcg gacacaagac
      961 cgtcgcggac atcttaaagg tgccgttcgt cgcggccgac ttaaacgacc gcacgacgct
     1021 gaccaccacg ctgcgcgaga acaagatcga agcggtcatg cacttcgccg cttacgcgta
     1081 cgtcggcgag agcgtcgaca agccgctgat gtactatcag aacaacgtag ccaccacgat
     1141 cagcgtgctg cagtcgatgc aggaagtcgg catcgatcgc ttcgtcttct ccagcacctg
     1201 tgcgacctac ggcgacccgg acaagatccc cattaccgaa gacgaaaaga aagccccggt
     1261 cagcccgtac ggccgcagca agtggatggt cgagcaaatc ctgcaggaca cccccggcgg
     1321 ctggaaggat ttcaaattcg cagcccttcg ctacttcaac gccagcggct gcgcgatgga
     1381 cgggtcgctc ggcgaagacc acgaccccga gacgcacctg atccccgtcg tgctgcaggc
     1441 gatcatgggt gttcgccccg gcatcacgct gttcggcacc gactatccga ccaaggacgg
     1501 caccaacgtc cgcgattaca ttcacgtcga cgacctggcc gacgcgcaca tcgccgcgat
     1561 ggagcggctg gacgagcaga agaagatcgt cgcgaatctc ggcaccggtc acggattcag
     1621 cgtcaaggaa atcatcgcga cggcggagaa ggtcaccggc aaaaaggtcc ccgtcaccta
     1681 cggcccccgc cggcccggcg atgcgatcgc gctgtacgcc gaccccacgc acgccaagcg
     1741 atcgctcggc tgggaagcga agtacaagga ccccgaaacg atcatccgct cggcgtggaa
     1801 ttggttttcg aaacacccga acggctacgg cgcccgaaaa taactcaaca ctggccttgc
     1861 gcgttcaatt ctgctgaaac ccccgtcgcg cttttgatgg cgcgattcgt ctcctatact
     1921 ccgcccgatg tcagaaaatc agacgatgct gctgcggctc gggcattcgc cggaccccga
     1981 tgacgcgttc atgttttacg ggctggcgaa ggacaagctg ccgaccggcc cctatcgctt
     2041 cgagcacgta ttgcaggaca ttcaaaccct gaaccgccgg gcgatgaacg gcgaactgga
     2101 gatcaccgca atctcgatcc acgcgtatcc gtatgtggcc gacaagtacg cgctgaccag
     2161 ctgcggcagc tcgatgggcg acaagtacgg gccgatggtc atcgcccgcg aagggatgcc
     2221 gctggaggcg ctgaagggca agacgatcgc gattcccggc gacctgacga ccgcgtttct
     2281 cacgctgcag ctttgcctgg gcaaaggcgc gaagcgcggc tcgagacagc gcggcgcatt
     2341 cgagtacgac gtcgttcact tcgacgagat tcccaaatac atccgcgacg gcaaagccga
     2401 cgcggggctc atcatccacg aaggccagct gacctatcaa acgctgggcc tgcagctgct
     2461 ggtggacctg ggcatgtggt ggcaccagcg cacggcactc ccgctgccgc tgggcgggaa
     2521 ctgcatccgc aaggacctgg gcccgcgcgt gatgcaggag gtgacggaca tcctcaagcg
     2581 atcgattcag tacagcctcg atcatcgggc cgaggcggtc gagtacgcgc tgcagttcgg
     2641 ccgggatctg gatcgcaagt tagcggacca gttcgtcggc atgtacgtga acgactggac
     2701 gctggattac ggcccgcgcg gccgcgaggc gatcacgagg ctattgaaag aaggcgccgc
     2761 cgcggggttg gtgccgaatg tgggggatgt tcagtacgtc acggccaagt aactttctcg
     2821 tcccctgcgg ggctaccgct acgcggagca cagagaaggt cgcatcgacg tgatgcattc
     2881 atcgcctgcg cgttgacgca cctcccccgc ctgacgacga tgcgatggtg attcgccgaa
     2941 cggcagtttt cttcgctctt tttctctctc tcctcgcatc gagcgcgtcg cccgcgttcg
     3001 cgcagccggc ggcggcgacg gctcggcaga cgcgcgatcc cctgcacgtg cttctcatca
     3061 cgatgggccc cggcgccgat ccgtgggaaa agtttggcca caacgccatc gagatcgtcg
     3121 atctcgagac cggttccgac cgctgctaca actggggcgt cttcagcttc ggcgaaggcg
     3181 tgagcggatt cctcggcttt acctggcact tcctgcaggg ccggctgtgg tattcgatgc
     3241 agtcagaccc ggcgggcccg atgttggagg cctacgccga cgccgggcga tcggtgctgg
     3301 ttcaggaact gcagatgacg cacgagcaga aggcggcgct gcaacaccgg ctgcaggcgc
     3361 aggacaccga tgcgaaccga tactacctgt acgattattt cctgaagaat tgttccacga
     3421 tggctcgcga tgcgatcgat cagacggtcg atggtcgcgt ggccgccgcg ttgcgcgatg
     3481 tgccgacggg cacgacctat cgctggcacg accgccgact gaccgcggat gaactttggc
     3541 tgtacacgtt tctggacttc gcgctgggtc atccgatcga tcatgagctg tcggagtggc
     3601 aggaatcgtt tctgccgatg aagttggcca cgcacctgcg ggaagtccgc gtgcccgacc
     3661 ccgatggcca cctggtgccg ctggtgatgt gggagaagca actcgcgccc ggggctttcc
     3721 aggaacgcga cacaccgccg cggta
//
LOCUS       DATIMH010000652         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1894428, whole genome shotgun sequence.
ACCESSION   DATIMH010000652 DATIMH010000000
VERSION     DATIMH010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894428"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VLI90_11290"
     CDS             complement(<1..487)
                     /locus_tag="VLI90_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14837.1"
                     /translation="MRAGSPDERGLFQAMVGDGRPLLLFTGVALILAGGFAIFQSATG
                     QFLPHDVEYLGMTAKDLCRLDQCRIVHFMFHDRVSFGGSLIAIGSLYLWLTEFPLREH
                     QPWAWWLLALSATVGFASFLTYLGYGYLDTWHGVATLLLMPFFAGGLYRSYFLLPRPT
                     HL"
     gene            complement(1073..1684)
                     /locus_tag="VLI90_11295"
     CDS             complement(1073..1684)
                     /locus_tag="VLI90_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14838.1"
                     /translation="MAKRAQKQTKSTKSTKATKHTGKVGDDPQALKNVGGKGDFGIPA
                     RDPVNRATDGVIEGRPAGSAPGYSGDAGVRTTGVGSMGGPPGHDSGGDLDPDYIGLDG
                     RGGLSAKPPREEELHGADMVQGQTGGDRSRKPRGRGKIGPGTHGAAPNAQGDYIDHSG
                     EDTSTNSPFEAGSVKPIRGDDPGAEAEVNLDEATGDLDQGAEQ"
     gene            complement(2020..3051)
                     /locus_tag="VLI90_11300"
     CDS             complement(2020..3051)
                     /locus_tag="VLI90_11300"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HSV14839.1"
                     /translation="MQRRCAPLAAFAAVGLLLLGLSVARADEVVFKNGDKLTGKITSL
                     GDGKLTIHTMEAGDVKVDAAQLQTFSTDAPITVRLSDGSTVVQKIDAADNGQVTIGGG
                     LLGNQHVNVSNVEAINPPSAVWSGDIKFGGLLIRGNTSSETVNFGMDVARTTEKDVIS
                     FGASYLYGRTKDRTTGLHTTTADNWQADLKYEYNFTKKFYGFAEAAVSRDRLAFLDLR
                     FNPSAGVGYRWFTKPNFTFATEGGIAWLYQHYTNSTPTREDVALKLAYHLTTKFNDYV
                     SLLHDLEYFPSVENGSVFVVTTDLGLRAALTTHFFTEFKFVLDYDSQPAVGALKTNTR
                     YELNVGYSI"
     gene            complement(3107..>3422)
                     /locus_tag="VLI90_11305"
     CDS             complement(3107..>3422)
                     /locus_tag="VLI90_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HSV14840.1"
                     /translation="IIMPVVSYVTPHMDFKEWHLGKLTFGVFLGDLLNFLIVALAIFL
                     IVVKVIGALMKKTAKPPPPPGEPAVKDCPFCLSTIPFKARKCGHCTADLPAEGAAVPA
                     LA"
BASE COUNT          632 a         1038 c         1112 g          640 t
ORIGIN      
        1 gcagatgggt cggccgcggc agcagaaaat acgagcgata caacccaccg gcgaagaacg
       61 gcatcagcag cagcgtcgcc acgccgtgcc aggtgtcgag gtagccgtaa ccgagatacg
      121 tcaggaacga cgcgaagccg accgtggcgc tgagcgcgag caaccaccac gcccacggct
      181 ggtgttcgcg caatggaaat tcggtcagcc acaggtagag cgagccgatc gcgatcagcg
      241 agccgccgaa cgacacgcga tcgtggaaca tgaagtgcac aatccggcac tggtcgagcc
      301 ggcacaaatc cttggccgtc atccccaggt actcgacatc gtgcggcagg aattgcccgg
      361 ttgcggattg gaagatcgcg aacccgccgg cgaggatcag agctacgccg gtgaacagaa
      421 gcaatggccg gccgtcaccg accatcgctt ggaagagccc gcgttcatcg ggtgatccgg
      481 cccgcatgga cgggattgta ccgcgacgcc gaaacacagg cataaaccag ccaccgacag
      541 atcgacgcgc atgccgccgg gaacaggcgt gaaacgggtc taagtaagcg ctactaactt
      601 cttccgaatg cgccacgaat gatcaatttc gcaacgaccg tcgttgaaga aacgcgtccc
      661 acgccgggtc gtcggagtat ccttcgactt cgctcaggac aggcctccga acccggcgtg
      721 gagggggcaa cgcttttcag cgtctccatg accaatcgga tttaatttgt cgcaactact
      781 ttagccgcag gcgacagcca gcgggggcgc gggcagtcgc gcggatttga attcgaaagc
      841 ctccgctggc tatcgcccgc ggctattacg atggtgtgaa caacgatggc ggcgccgaac
      901 cgccaaacgc tggcgacgcg cagcatcagc cgggcctgcg cgcgcgggcg atgcgccaga
      961 cgcagaagac gaagacgatc acaccagtcg cgcacgaccc gatgccgaaa tcgggccgca
     1021 tcaatctccg cgcagcgcga gggcgctcgc gggcgcgacg cggtcagttc cgctactgct
     1081 ccgccccttg atcgaggtcg cctgtcgctt cgtcgaggtt tacttcggct tcggcgccgg
     1141 ggtcgtcgcc gcggatcggt ttgacggagc cggcttcgaa ggggctgttg gtgctggtgt
     1201 cttcgccgct gtgatcgatg tagtcacctt gcgcgtttgg cgcggcgcca tgggtacccg
     1261 ggccgatctt gccgcggccg cgcggcttgc gcgatcgatc tccacccgtt tgaccctgca
     1321 ccatgtcggc gccgtgcaac tcttcctcgc gcgggggttt ggcggacagg ccgccacggc
     1381 cgtcgaggcc gatgtaatcg ggatcgaggt cgccaccgct gtcgtggccc ggcggcccgc
     1441 ccatgctgcc gacaccggtg gtgcggacac cggcgtcgcc gctgtatcca ggggcgctgc
     1501 ccgcggggcg gccttcgatc acaccgtcgg tggcccgatt gacggggtcg cgggcaggga
     1561 tgccgaagtc gcccttgccg ccgacgttct tcagggcctg cgggtcatcg ccgacctttc
     1621 cggtgtgctt tgtggctttc gttgatttag ttgacttggt ttgcttttga gctcgcttgg
     1681 ccatcaggtg ctcctttgga gaatccatgg agcgggtagc aagcggcgaa ccgcgcgggg
     1741 cgcgtgcgtg cgcgcgctgt catcctccag cgcaggcgta ctcgtgcctc gactgagctc
     1801 gccgaggtcg cgatcgaagg atctagccga gaaggaggaa tgatcgcgcg atcggcgcgg
     1861 gagtagcttg cccttggcca ggaacgccgc ggcgaatccg cgaggggcaa gcgacttcta
     1921 gaccactcgc gccgacaaac agttcaaata tcgcggcaag atccttcgac tgcgcacgag
     1981 tacgtgctgc gcccaggatg acgcgcgcgt gcgtacgcgt tagatcgaat accccacgtt
     2041 taactcgtat cgcgtgttcg tcttcagcgc gcccaccgcc ggttgggaat cgtagtcgag
     2101 cacgaacttg aactccgtga agaagtgggt tgtcagggcc gcgcgcaagc cgagatctgt
     2161 ggtgacaaca aacacgctgc cgttttcgac gctggggaaa tattcgaggt cgtggagcag
     2221 cgagacgtag tcgttgaact tcgtcgtcag gtggtaggcc agcttcagcg ccacgtcttc
     2281 gcgggttggc gtgctattgg tgtagtgctg gtacagccag gcgatgccgc cttcggtcgc
     2341 gaaggtgaag tttggttttg tgaaccagcg ataaccgacg ccggcggatg ggttgaagcg
     2401 cagatcgagg aacgccaggc gatcgcgcga caccgcggct tcggcgaatc cataaaactt
     2461 cttcgtgaag ttgtattcgt atttcaggtc tgcttgccaa ttgtcggcgg tcgtggtgtg
     2521 caggccggtc gtgcggtcct tggtgcggcc gtacaggtag ctggcgccga agctgatgac
     2581 gtccttctcg gtcgtgcggg ccacgtccat gccgaagttg acggtttccg acgaggtgtt
     2641 cccgcggatg agcaggccgc cgaatttaat atcgccgctc cagacggcgg atggcgggtt
     2701 gatcgcctcg acgttgctga cgttgacgtg ctggttgccg agcaacccac caccgatcgt
     2761 cacctggccg ttgtccgcgg cgtcgatctt ctgcacgacg gtggagccat cgctcagccg
     2821 cacggtgatc ggcgcgtcgg ttgaaaacgt ctgcagttga gcggcgtcca ccttcacatc
     2881 gccggcttcc atcgtgtgga tggtgagctt tccatcaccc agcgacgtga ttttgcccgt
     2941 gagcttgtcg ccgtttttga aaaccacttc atccgcccgg gcgacactga ggccgagcag
     3001 caacagaccc acggcagcga atgccgccag gggagcgcat cgtcgttgca tttagatttc
     3061 tccgattaag ttcccacgcc cgagacagcc cattcgaaac tggcgatcaa gccaacgccg
     3121 ggacggcagc gccttctgcc ggcaggtctg cggtgcagtg cccgcacttg cgcgccttga
     3181 acggaatcgt cgacagacag aacggacaat ccttaacagc cggttcaccc ggtggcgggg
     3241 gcggtttggc ggttttcttc atcagcgcgc cgatcacttt caccacgatg aggaagatcg
     3301 ccagcgcgac gatgaggaag ttcagcaggt cgcccaggaa gacgccgaac gtcagcttgc
     3361 ccaggtgcca ctccttgaaa tccatgtgag gcgtgacgta gctgactacg ggcatgatga
     3421 tg
//
LOCUS       DATIMH010000653         8204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4489166, whole genome shotgun sequence.
ACCESSION   DATIMH010000653 DATIMH010000000
VERSION     DATIMH010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4489166"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VLI90_11310"
     CDS             <1..202
                     /locus_tag="VLI90_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14841.1"
                     /translation="FNDLPSWQKRGAGLYWEAYDKEGFNPQTGQNVRAIRRRIKRDIE
                     LPMKEAYSEFIAAIVAREQSST"
     gene            199..2787
                     /locus_tag="VLI90_11315"
     CDS             199..2787
                     /locus_tag="VLI90_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HSV14842.1"
                     /translation="MKLTVPELSLVVLIGPSGSGKSSFARKHFKPTEVISSDFCRGLV
                     SDDENSLDATGDAFDVLRFVAAKRLARGLLTVIDATNVQPESRKVLVELAREHDVLPV
                     AIVLDVPEHVCRQRNEARPDRNFGSHVVRNQLMQMRKSLRFLDREGFRYVHRLSCQIT
                     SSRDVLIDDVAIERQPLWNNRKWDRGPFDIIGDVHGCYDELIELLNQLGYDTTEINQI
                     VPPPGRKAVFLGDLVDRGPKTPEVLRLVMSMVVGNVALCVPGNHDEKLLRKLRGRNVQ
                     IAHGLAETLQQLEGDPSLMASAEKFISQLVGHYVLDGGNLVVAHAGMKEQYIGRASQR
                     VREFALYGETTGETDEFGLPVRFNWAAEYRGNAAIVYGHTPVPEADWLNRTINIDTGC
                     VFGGKLTALRWPERELVSVPAKRVYAEPSKPFLPVEAATNVVASDLSAQQLHDDVLDL
                     SDVLGKRIVSTRLMHNVTVREENATAALEVMSRFAANPKWLIYLPPTMSPTETTAEPG
                     LLEHPREAFNYFLHHGVGQVMCQEKHMGSRAVVIVCRDASAAMRRFGVRDDGIGIIYT
                     RTGRRFFEDRAVEAELLAHVHRAIDRANLWDELASDWFCLDCELMPWSAKAQDLLKNQ
                     YAAVGSASRAALAETVSTLGRATERYRSMGDESVEALLQQHQQRLGMAEQYVEAYRRY
                     CWPVNSVADLKLAPFHLLASEGHVHTDKDHQWHMQTLAKLADGASPLMATNHRLVDVT
                     DPASVAGGIAWWAELTARGGEGMVIKPLEFCVRGKKGLVQPAIKCRGREYLRIIYGPE
                     YAAEANLQRLRSRGLGAKRSLALREFALGIEALERFVRREPLRRVHECVFAVLAMESE
                     PVDPRL"
     gene            3107..3670
                     /locus_tag="VLI90_11320"
     CDS             3107..3670
                     /locus_tag="VLI90_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV14843.1"
                     /translation="MAWRFSQPGGGRWRAAAPGRRAFSLLELLVVIGIIAMLIAMLLV
                     ALRRARTAAQSINCISNLHQIATGLRMFANDNGNRFPDPGALEVSWESTIRKYLQNSK
                     VFECPGDEELCPTAGSSYDWRDTSVPETTLAGRSMTDVRRQDLVLAFEALPGWHFKKK
                     MNVSRIDGSCCTMDDQEAVGDLLKPVQ"
     gene            3735..4499
                     /locus_tag="VLI90_11325"
     CDS             3735..4499
                     /locus_tag="VLI90_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSV14844.1"
                     /translation="MRILVAEDRPALARSLSDGLRDEGYVVDLTLSGTEADHLARANP
                     YDCAVLDIMLPGMEGWDVLSRMRAAGVKTPVLLLTARDAVADRVKGLNLGADDYLVKP
                     FAWEELLARVRSLIRRTHGQESSVLTIADLEIDTAAKSVRRAGREIVLTAREYALLQY
                     LAMRRDQVVSRTEIWEHLYDQDDQSSSNVVDVYIGYLRNKLDRPFGTSLIHTRRGLGY
                     MLSDKSAASNVATPPAPAAASSSAPQSPVGQGGAIG"
     gene            4496..5863
                     /locus_tag="VLI90_11330"
     CDS             4496..5863
                     /locus_tag="VLI90_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV14845.1"
                     /translation="MTRRIAIAILLTTWTALIAIGVTVYLATRHVLLTDLDASVVARA
                     ASLPDALRVPGATAAENAASVPEGDRFIIRNAVGRTLARPATAAHGATPVVMARAFSR
                     LGDGSRVRTLTLVIGEDDASNADKRLVVTYSASAARFDHLLGQLLMWLIASGIVCGCG
                     AAAAAVVLSRIALRPLRQTADVIGAIDERQLARRIEADSLPPELRPMAGRLNEMLARL
                     ETSFEERRRFLAAASHELRTPVAAILTTIEVALRRPRGAEEMTESLRACLPDAKVLRQ
                     LVDWLMQQVRSESPSLSEPRQRFDAVALLEECADAALRAAGDRPITIRRHLPERLDVY
                     TETNRLRSIIMNLLGNAMEYGRADGQIELRCEVTGDAIIVRISDDGPGIAEELLPRLF
                     EPFSRGDARQAADIHLGLGLSLVQAHARALGGRCEVQSAVGAGSTFIVRLPCSILASP
                     DQLVA"
     gene            5932..6369
                     /locus_tag="VLI90_11335"
     CDS             5932..6369
                     /locus_tag="VLI90_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14846.1"
                     /translation="MGLETVLKRNRFILLMLVALAMLAGPAFQAAGKPGSPAKTKGSY
                     DFVFAGAVEGDGHGAVAAQSVNIIGKVKDTKTGATSNFNATNLQMDNGRFSGTGTAFG
                     VTVTVNGRVEDADGVTVLLPRVFCTYDIPGLGNGRIVGERKGP"
     gene            6747..>8204
                     /locus_tag="VLI90_11340"
     CDS             6747..>8204
                     /locus_tag="VLI90_11340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV14847.1"
                     /translation="MLRGDDRACARAAGTTDARVSAAGKFFELDGQRWLLKGLTYGPF
                     APNSNGDYMPERPRMAADFAQARALGANCLRVYHKPTPQLLELALEHDLRIFVDVPWE
                     KHRCFFEDWTALQDARQRVRTTARELGGLPGLFALSVVNEIPSDVVRFYGRRRVAQFV
                     EELIDIVKQEAPTCLTTFANYPSTEFLRPDAGDFHCFNVYLKDGQTLGSYLDRLQHLA
                     GSKPLILGEFGADSFRSGEARQAEAIAEHVSRVFHHGLAGSFVFSFTDDWFTGGHQIE
                     DWAFGVTRLDRSEKPAAITLKRAWARAPRVESIPTPRVSVVVCSYNGAATLRECLDSL
                     VRLNYPDYEVILVDDGSRDSTPEIAADFPSIRTIRQENLGLSVARNVGAEAATGEIVA
                     YTDSDCVADDQWLRYLVTAMRDQQVQAIGGPNVPPASDRWVAKCVAASPGGPSHVMLD
                     DRYAEHVPGCNMAFDRNRLLELGGFDAQFRVAGDDV"
BASE COUNT         1467 a         2382 c         2841 g         1514 t
ORIGIN      
        1 cttcaacgat ctgccgagtt ggcagaagcg tggagcggga ctgtactggg aggcgtacga
       61 caaggagggt ttcaatcctc agactgggca gaacgtgcgg gcgatacgcc ggcggatcaa
      121 gcgggacatc gaactcccga tgaaggaagc ttacagcgag ttcattgccg ccatcgtcgc
      181 gcgcgaacaa tcatccacat gaagctcacg gtcccagaac tttcgctcgt cgtcctgatc
      241 ggccccagcg gctcgggcaa gtccagcttt gcgcgcaagc atttcaagcc gaccgaggtg
      301 atctcctccg acttctgccg gggcttggtc agcgatgacg agaacagcct ggacgcgacc
      361 ggcgatgcgt ttgatgtgtt gcggtttgtg gcggcgaagc gattggcgcg cgggctgctg
      421 accgtcatcg atgcaacgaa tgtgcagccg gagtcgcgta aggtgctggt ggagttggcg
      481 cgcgagcacg atgtgctgcc ggtggcgatc gtgctggatg tgccggagca cgtgtgtcgc
      541 cagcggaacg aggcgcggcc ggatcgcaac ttcggcagcc acgtcgtccg caatcagctg
      601 atgcagatgc ggaagtcgct gcgctttttg gatcgcgagg gttttcgcta tgtccaccgg
      661 ctcagctgcc agatcaccag ctcgcgcgac gtgctgatcg acgacgtggc catcgagcgg
      721 cagccgttgt ggaacaaccg caagtgggac cgcgggccgt tcgacatcat tggggatgtg
      781 cacggctgtt atgacgagct gatcgagttg ctgaaccagc tgggatacga caccacggag
      841 atcaaccaga tcgtgccgcc gccggggcgg aaggcggtgt ttctggggga cctggtcgat
      901 cgcgggccca agacgccgga ggtgttgcgg ctggtgatgt ccatggtcgt gggcaacgtc
      961 gcgctgtgcg tgccggggaa ccacgatgag aagctgttgc ggaagctgcg cgggcggaac
     1021 gtgcagatcg cgcacgggct ggccgagacg cttcagcagc ttgagggcga tccgagcctg
     1081 atggcatccg ctgaaaaatt catcagccag ctggttggcc attacgtgct cgatggtggc
     1141 aatctcgtcg tcgcgcacgc ggggatgaag gagcaataca tcggtcgcgc gagccagcgc
     1201 gtgcgcgagt tcgcgctcta tggcgagacg acgggtgaga ccgatgaatt cggcctgccg
     1261 gtgcgcttca actgggcggc ggaatatcgc ggcaacgcgg cgatcgtcta tggccacacg
     1321 ccggtgccgg aggcggattg gctgaatcga acgatcaaca tcgacaccgg ttgcgtgttc
     1381 ggcgggaagt tgacggcgct gcgctggccg gagcgagaat tggtttcagt gccagccaag
     1441 cgcgtgtacg ccgagccatc caaacccttt ctgccggtcg aggcggcaac caatgtagtg
     1501 gcgtcggatc tttccgcgca gcagctgcac gatgatgttc tggacctgtc cgacgtgctg
     1561 ggcaagcgaa tcgtcagcac taggctgatg cacaacgtga cggtgcgcga ggagaacgcc
     1621 acggccgcgc tggaagtgat gagccggttc gcggccaatc ccaagtggtt gatctatctt
     1681 ccgccgacga tgtcaccgac ggaaacgacg gccgagccgg gcttgctcga acatccgcgc
     1741 gaggcattca actattttct tcatcacggc gttggtcagg tcatgtgcca ggagaagcac
     1801 atgggcagcc gcgccgtcgt catcgtctgc cgcgatgcat ccgccgcgat gcgacgcttt
     1861 ggcgtgcgcg acgacggcat cgggatcatc tacacgcgca cgggccggcg gttcttcgaa
     1921 gatcgcgcag ttgaggcgga gttgctggcg cacgtgcatc gtgcgatcga tcgggcgaac
     1981 ctttgggacg agcttgccag cgactggttc tgcctcgact gcgagctgat gccttggtcg
     2041 gccaaggcgc aggacctgct gaagaaccaa tacgccgcgg ttggatcggc atcgcgcgct
     2101 gcgctggcgg agacggtctc aacgctcggg cgggccacgg agcgatatcg ctcgatgggc
     2161 gatgaatcgg ttgaagcgct gctgcagcag catcagcagc ggctggggat ggccgagcaa
     2221 tatgtcgagg cgtaccgccg ctattgctgg ccggtgaatt cggtcgcgga cctgaagctg
     2281 gcgccgtttc atctgctggc cagtgaaggt catgtacaca cggacaagga tcaccagtgg
     2341 cacatgcaga cgctggcgaa gctggcggat ggcgcctctc cattgatggc gaccaaccat
     2401 cgtctggtcg atgtgacgga tccagccagc gtcgccggcg gcattgcgtg gtgggcggag
     2461 ctgaccgcgc gcggtggcga ggggatggta atcaagccgc tcgaattctg cgtgcgcggc
     2521 aaaaagggac tggtgcaacc ggccatcaaa tgccgcggac gggaatactt gcgtatcatc
     2581 tacggtccgg aatacgcggc cgaggcgaac ctgcagcgcc tgcgcagccg cgggctgggc
     2641 gcgaagcgtt cgctggcgct gcgcgagttt gcgctaggca tcgaagcgct ggagcgattc
     2701 gtccgccgcg agcccctgcg gcgggtgcac gagtgcgtgt ttgccgtgct tgccatggaa
     2761 agcgaaccgg tagacccgcg gttgtgagat gcattacgct cggtcgattt tcaagacaca
     2821 gcctcgttgg gctcgcccgc aggggttgta taggcggcat gggcggcaaa tattgtcgga
     2881 ccgttccggg ctaccggtcg ggtttcacta ccacgtttca atagaatacg gataatccat
     2941 gtccgctggc gatcaggatg cgcacacgcg cgcgttccgc ccgtgggtgt atgggtgatg
     3001 gttcagggat gccgctgacg ccgcgcgcgc acacgcgaac atgcgcgcgg gcggcgatgg
     3061 cggcgcgatc cggcagtggc ggcgggcagc gcctccggga cgatcgatgg catggcgttt
     3121 ttcacaacca ggcgggggtc gatggcgcgc cgccgcaccc ggccggcgcg cgttctcgct
     3181 tttggagctt cttgttgtca ttggaattat cgccatgctc attgccatgc tgctggtggc
     3241 gctgcggcgg gcgcgcaccg cggcgcagtc gatcaactgc atctccaacc ttcatcaaat
     3301 cgcgacgggc ctgaggatgt tcgccaacga caatggcaat cgctttccgg atccgggcgc
     3361 gctggaagtg tcgtgggaat ccaccattcg gaaatatctg cagaacagca aggtcttcga
     3421 atgcccgggt gacgaagagc tctgccccac cgccgggtct agttacgact ggcgagatac
     3481 atcggtgccc gagaccacgt tggctggtcg atcgatgaca gacgtgcggc ggcaggacct
     3541 ggtgctggca tttgaagcgc tgcccggttg gcatttcaag aaaaagatga acgtcagccg
     3601 catcgatggt tcctgctgca cgatggatga tcaggaggca gtcggtgatt tgttgaagcc
     3661 cgttcaatga cgcgggcacc cacgcacgca cgcacgcgca cgggcatggc ctgaaacgta
     3721 atggaggttc gcacgtgagg attttggtag cagaagatcg cccggcgttg gcgcggtcgt
     3781 tgtcggatgg gctgcgcgac gagggatacg tcgtggactt gacgctcagc ggaacggagg
     3841 cggatcatct ggcgcgcgcg aatccgtacg actgcgcggt gctggacatc atgttgccgg
     3901 gcatggaggg atgggacgtg ctgtcgcgca tgcgggcggc cggtgtgaag acgccggtgc
     3961 tgctgctgac cgcgcgcgac gcggtggccg atcgcgtcaa gggattgaac ctgggggcgg
     4021 acgattacct ggtgaaaccg ttcgcgtggg aagagttgct ggcgcgggtg cggtcgctga
     4081 tccgccggac gcacgggcag gaaagttcgg tgctgacgat cgccgatctg gagatcgaca
     4141 cggccgccaa gagcgtccgc cgcgccggcc gcgagatcgt gctgaccgcg cgcgagtacg
     4201 cgctgctgca atacctggcg atgcggcggg accaagtcgt ctcgcgcacc gagatctggg
     4261 aacacctgta cgaccaggac gaccagagca gcagcaacgt ggtcgacgtc tacatcggat
     4321 acctgcggaa caagctggac cgcccgttcg gcacgagcct gatccacaca cgccgcgggc
     4381 tgggttacat gctgagcgat aaatcagcag cgtcgaacgt ggcaacgcca ccggcgccgg
     4441 cggcggcgtc cagttcggca ccgcaatcac ccgtgggtca agggggcgcg atcggatgac
     4501 gcggcgaatt gccatcgcga tcctgctgac gacgtggacg gcgctgattg ccatcggcgt
     4561 gaccgtctac ctcgcgacgc ggcacgtact gctgacggat ctggacgcgt ccgtcgttgc
     4621 gcgggcggcg tcgttgcccg atgcgctgcg cgtgccgggg gcgacggcag cggagaacgc
     4681 ggcgagcgtg ccggagggcg atcgcttcat cattcgcaac gcggtgggcc gcacgttagc
     4741 gcggccggcg acggcggcgc acggggcgac gccggttgtc atggcgcgtg cgtttagtcg
     4801 attgggcgat ggcagccggg tgcgaacgct gacgctggtg attggcgagg acgacgcgag
     4861 caacgctgac aagcgcctgg tggtgacgta cagcgcatcg gccgcgcgct tcgatcacct
     4921 gctgggccaa ctgctgatgt ggctgatcgc cagcggcatc gtctgcgggt gcggcgcagc
     4981 ggcggcggcg gtggtgctgt cgcgcatcgc gctgcggccg ttgcgacaaa cggccgacgt
     5041 catcggcgcg atcgacgagc ggcagctcgc gcggcgcatc gaagcagact cgctgccgcc
     5101 ggaactgcgc ccgatggcgg gacgtttgaa cgagatgctg gcgcggctgg agacatcgtt
     5161 cgaagagcgc cggcggtttt tggcggcggc atcgcacgaa ctgcgcacgc cggtcgcggc
     5221 gatcttgacc acgatcgaag tcgcgctgcg ccggccgcgc ggcgcggagg agatgaccga
     5281 atcacttcgc gcgtgcctgc cggatgcgaa ggtgcttcgg cagttggtgg actggttgat
     5341 gcagcaggtg cggagcgaat cgccatcgct tagcgaaccg cggcaacggt tcgatgcggt
     5401 tgcgctgctg gaggagtgcg ccgatgcggc gctgcgagcc gcgggcgatc ggccgatcac
     5461 cattcggcgg catttgccgg agcgactgga cgtatacacc gaaacgaatc gtctgcgcag
     5521 cattatcatg aacctgctcg gcaatgcgat ggaatatggg cgggcggatg gacagatcga
     5581 gctgcgatgc gaagtgacgg gcgacgcgat catcgtacga atatcggacg acggcccggg
     5641 catcgccgaa gaactgctgc cgcggttgtt cgaaccattc tcccgcggcg acgcgcggca
     5701 ggcagcggat attcacctgg ggcttgggct gtcgctggtg caggctcacg cccgggcgct
     5761 ggggggtcga tgtgaagtac agagcgcggt tggagcgggc tcaacgttta tcgtgcgatt
     5821 gccgtgctca attctggcat cgccggacca gttagtcgca tgaatgggca tcgcttcatc
     5881 cccctctcat gcgcggttga tagattgtgg tgtggagcga cgtttgcggt cttggggtta
     5941 gagactgtgc taaagcggaa tcgattcatc ctcctgatgc tggtcgcgct ggcgatgctg
     6001 gcggggcccg cgtttcaggc ggctggtaaa ccgggcagcc cggccaagac caagggctcg
     6061 tacgatttcg tctttgctgg cgcggttgag ggcgatggtc acggcgccgt cgcggcgcaa
     6121 tcggtcaata tcatcggcaa ggtgaaagac accaagaccg gggcgaccag caatttcaac
     6181 gccacgaacc tgcagatgga caacggccga ttcagcggca ccggcacggc gttcggtgtg
     6241 acagtcaccg tcaacggccg cgtggaagac gccgacggcg tgacggtcct gttgccgcga
     6301 gtcttctgca cgtacgacat ccccggcctt ggcaacgggc gaatcgtcgg cgagcgcaaa
     6361 ggtccctaat gtgacgcgag ccgcggcgcg cacctgctaa ttttgtagtc tcacagtcat
     6421 ctcacctcgg gttcggtaga ttttgaagcg gaccgggcaa ttgcttcgaa cccacccatc
     6481 gcagcgcacc cacacagcga tggtcgaggc acacccggtt ctttttcgtg gataccttat
     6541 ttcttttcca acttatgtga gattctcact atctcctcat cttgtgcgat taacattttc
     6601 cgcgggcatc aaccgaccag caggctgggt ttgaacgaaa tcgggctttt gttccgcacg
     6661 cttcgttctt agatcgtgag acgtgggtga atggacttgt taacaattta gtgcccccgc
     6721 catcggcgcg ggcacacgaa ggtcatttgc ttcgcggcga tgaccgcgcg tgcgcccgcg
     6781 ctgccggcac tactgatgcg cgcgtcagtg cagcggggaa gtttttcgaa ctggatggcc
     6841 agcggtggct gcttaaaggt ttgacgtatg gtccgttcgc gccaaacagc aatggcgatt
     6901 acatgcccga gcgcccgcgg atggccgcgg acttcgcgca ggcccgcgcg ctgggcgcga
     6961 actgcctgcg cgtctatcac aagcccacgc cgcaactgct cgagctggcg ctggaacacg
     7021 acctgcggat tttcgtcgat gttccgtggg agaagcatcg ctgctttttc gaggactgga
     7081 ccgcactgca agatgcgcgt cagcgcgtgc gcacgacagc ccgggagtta ggagggctcc
     7141 cgggcttgtt cgcgctcagc gtcgtcaacg aaatcccgag cgacgtcgtc cgcttctatg
     7201 gccgccggcg cgttgcgcag ttcgtcgaag agctgatcga catcgtcaag caggaagcgc
     7261 caacctgcct cacgactttt gcgaattacc catcaacgga gttccttcgg cccgacgcgg
     7321 gcgacttcca ttgcttcaac gtttacctca aagacggaca aacgctgggc agctatctgg
     7381 atcgcctgca acacctggcc ggatcgaaac cgctgatcct cggcgaattc ggcgccgact
     7441 cgttccgcag cggcgaagcg cgccaggccg aagcgattgc cgagcacgtc tcccgcgtgt
     7501 tccatcacgg ccttgccgga tcgtttgtgt tttctttcac agatgactgg ttcaccggtg
     7561 gtcatcagat cgaagactgg gcgttcgggg tgacgcggct agatcgcagc gaaaaaccgg
     7621 cggcgattac cctgaagcgc gcatgggctc gcgccccgcg ggttgaatcg attccgacgc
     7681 cgcgcgtgtc ggtcgttgtg tgctcgtaca acggggccgc cacgctgcgc gagtgtctgg
     7741 attcgctggt gcgactgaat tatccggatt acgaagtgat cctggtcgat gacggctcgc
     7801 gcgattcaac gccggagatc gcggcggact ttccgtcgat tcggacgatc cgccaggaga
     7861 acctcgggct gagcgttgcg cgcaacgtcg gcgccgaagc ggcaacgggt gaaatcgtcg
     7921 cgtacaccga ttccgactgc gtcgcggacg accagtggct gcggtacctg gttactgcga
     7981 tgcgggacca gcaggtgcag gcgatcggag ggcccaacgt cccgccggcc agcgaccggt
     8041 gggtggcgaa atgcgtcgcc gccagccccg gcgggccgag ccacgtcatg cttgacgacc
     8101 gctatgccga gcatgtgccg ggatgcaaca tggctttcga tcggaataga ctcctcgaat
     8161 tgggaggctt cgacgcgcag ttccgggtgg cgggcgacga cgtg
//
LOCUS       DATIMH010000654         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1317903, whole genome shotgun sequence.
ACCESSION   DATIMH010000654 DATIMH010000000
VERSION     DATIMH010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317903"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VLI90_11345"
     CDS             complement(<1..330)
                     /locus_tag="VLI90_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14848.1"
                     /translation="MKFPTNASPVFYIPHERNLTFTGRTELMAELHTRFDKGERAQAI
                     HGLGGIGKTQTAIEYAYRHRDDYRFVLWAQANSRETLFADVAAIARRLKLARPEEPDP
                     AVIVRAVR"
     gene            complement(501..1544)
                     /gene="rlmN"
                     /locus_tag="VLI90_11350"
     CDS             complement(501..1544)
                     /gene="rlmN"
                     /locus_tag="VLI90_11350"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HSV14849.1"
                     /translation="MPSLVDHDLASLEMDLQAAGHKPVHALRVLRHFYANAGHVDLEQ
                     LTIGSGAREHLRHNIGPRRSTVTRHTLAADGTAKFLIGFASGGAAEAVLMLSHREGVA
                     AGCVSSQIGCAMGCDFCASTRGGFERDLDAGEIVEQFLHLKEQAAHIGRRLRTLVFMG
                     MGEPLLNFDNVVAAIRRIGDPNLGGLGWRQMTISTVGIVPAIDRLAELDLNVHLAVSL
                     HAPDDATRSRLVPMNRRHTVADVMNAARRFETRTGRTPTIEYCMLAGVNDSDEQAMLL
                     ADLMEGFRAHVNLIPYNPIGVGLRGVAYSRPTPERMNAFIELLRSHRVVTHFRAPRGD
                     DVNAACGQLRANS"
     gene            complement(1646..2557)
                     /locus_tag="VLI90_11355"
     CDS             complement(1646..2557)
                     /locus_tag="VLI90_11355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV14850.1"
                     /translation="MNATVVITTKNRKEDLRVAVRSALDQAAHPEVLVVDDGSTDGTA
                     DMIRSEFPTVRLERAEQSQGYIVQRNRGAELARGDVIFSIDDDAAFSTPHVVEQTLVE
                     FNHPRVAAVAIPFINVNKENMLYQRAPGDDAARIYVNYEFIGTAHAVRRDIFLKLGGY
                     REQLVHQGEERDYCVRLFDAGYIVRLGRSDPIHHFESPRRDYRRMDHFGRRNDVLYAI
                     HNVPFPYWPAHAAMTTLKGLWFGVKVQRFWRMARGLASGWGALPREWRDRRPIAPSAY
                     RLLRKLRRVHSLPLDEIESTLPPPRFG"
     gene            complement(2554..>2862)
                     /locus_tag="VLI90_11360"
     CDS             complement(2554..>2862)
                     /locus_tag="VLI90_11360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSV14851.1"
                     /translation="RHVAIGPGCYITDHDHGLDPSRPPLAQQMIAAPTRIEDWVWIGA
                     NAVILKGVTIGQRTIIGAGSVVTRDLPPNVIAVGVPARVVRQREASDEPTDLARAAQR
                     "
BASE COUNT          479 a          886 c          999 g          498 t
ORIGIN      
        1 gcggacggcg cggacgatca cggccgggtc gggctcctcg ggccgggcga gcttgagccg
       61 gcgggcgatc gcggccacgt cggcgaagag ggtctcgcgc gagttggcct gggcccagag
      121 gacgaagcgg tagtcgtcgc ggtggcggta ggcgtattcg atggccgtct gcgttttgcc
      181 gatgccgccc aggccgtgga tggcctgcgc gcgttcgcct ttgtcgaagc gagtgtgcag
      241 ctccgccatc agttcggtgc ggccggtgaa agttaggttt cgttcgtgcg ggatgtaaaa
      301 gacgggcgac gcgttggttg gaaacttcat ggagcagccc cgtaagttcg acgccggcgg
      361 cgaaggcccc cgcaaccatc gcgggcagca tattctagca ggtagacacc gcagagtccc
      421 aaaggtgggt gggatccgga aaagtggcac gggcgtctcg ccccgtgtca gggcaccttg
      481 gcacgggcga gacgcccgtg ctacgaattg gcccgcaatt gcccgcaagc ggcgttgacg
      541 tcatccccgc gcggggcacg aaagtgggtc acaacccggt gggagcgcag cagttcgata
      601 aatgcgttca tgcgctctgg ggtcggacga ctgtaagcca cgccgcgcag gcccacgccg
      661 atcgggttgt acgggatcag attcacatgc gcgcgaaagc cttccatcaa gtccgccagc
      721 agcatcgcct gctcgtcgga atcgttcacg ccggccaaca tgcagtactc gatcgtcggc
      781 gtccgcccgg tgcgagtctc gaaccgccgg gcggcgttca tcacgtcggc tacggtgtgc
      841 cggcggttca tcggcacgag ccgcgatctt gtcgcgtcgt ccggggcgtg cagcgagacg
      901 gccaggtgga cgttcaggtc cagctccgcc agccgatcga tcgcggggac gatgccgacg
      961 gtggagattg tcatttgccg ccacccaagc ccgcccaggt tcggatcgcc gatccggcga
     1021 atcgccgcga cgacgttgtc gaagttcagc agcggctcgc ccatgcccat gaagaccagc
     1081 gtgcgcagcc gccgaccgat gtgcgccgct tgttctttca gatgcaggaa ctgctcgacg
     1141 atctcaccgg cgtctaggtc gcgttcgaac ccacctcgcg tgctggcgca gaagtcgcag
     1201 cccatggcgc agccgatctg tgacgagacg cagccggccg cgacgccttc gcgatgggac
     1261 agcatcaaca cggcttcggc ggcgccgccg ctggcgaagc cgatgaggaa ctttgcggtg
     1321 ccgtcggcgg cgagggtgtg tcgcgtgacc gtcgagcgac gtgggccgat gttgtggcga
     1381 agatgttctc gcgcgccgct gccgatcgtc agttgttcga gatcgacgtg accggcgttg
     1441 gcgtagaagt ggcgcagcac gcggagcgcg tgaaccggct tgtgacccgc ggcctgcagg
     1501 tccatttcga gcgaggcaag atcgtgatca accagcgacg gcattgcggc gatgagtcta
     1561 ccgcatttgc cgcttgcggc ttagcgtctg tttcttctcc gggaaagaaa cagacgctaa
     1621 gccgcaagcg gcgggggcgc accgatcatc caaaccgcgg cgggggtagg gtggattcga
     1681 tctcatccaa tggcagcgaa tgcacgcggc gaagctttct cagcaggcga tacgcggacg
     1741 gcgcgatcgg ccgcctgtcg cgccattcgc gcggcagcgc gccccatcct gatgcaagcc
     1801 cgcgggccat gcgccagaag cgctgaactt tcacgccgaa ccataatcct ttcagcgtcg
     1861 tcatcgccgc gtgagcgggc cagtacggaa acggcacatt atgaatcgcg tacagcacgt
     1921 cgttgcgccg gccgaagtgg tccatccggc gatagtcccg gcgcggcgat tcgaaatggt
     1981 ggatcgggtc ggaccggccg aggcgcacga tgtatccggc gtcgaacagg cgaacgcaat
     2041 agtcgcgctc ttcgccctgg tgaaccagtt gctcgcgata gccgccgagc ttcaggaaga
     2101 tgtcccgccg aacggcgtgc gcggtgccga tgaattcgta gttgacgtaa atgcgggcgg
     2161 cgtcgtcgcc gggcgcgcgc tgatacagca tgttttcctt gttgacattg atgaacggga
     2221 tggcgacggc ggcgacgcgc gggtggttga actccaccag cgtctgctcg acgacgtgcg
     2281 gcgtggaaaa ggccgcgtcg tcgtcgatgg agaagatcac gtcgccgcga gcgagctcgg
     2341 cgccgcggtt gcgctgcacg atgtaccctt gcgattgctc ggcgcgctcc agccggaccg
     2401 ttggaaattc gctgcgaatc atgtcggcgg tgccgtcggt ggaaccgtca tcgacgacga
     2461 gcacttccgg gtgcgccgcc tgatcgagcg cggatcgaac ggcaacgcgc aggtcttcct
     2521 tgcggttttt ggtggtgatg acgacggtgg cattcatcgc tgcgccgccc tggccaggtc
     2581 ggtgggttcg tcgctcgcct cgcgctggcg gactacccgg gcgggcacgc ccacggcgat
     2641 cacgttcggc ggcaaatcgc gggtgacgac gctgccggcg ccgatgatcg tacgctgccc
     2701 gatcgtcacg cccttgagga tcaccgcatt ggcgccgatc cagacccagt cttcgatccg
     2761 cgtcggagcg gcgatcatct gctgcgccag cggcgggcgc gatggatcga gcccgtgatc
     2821 gtgatcggtg atgtagcaac cggggccgat ggccacatgc cg
//
LOCUS       DATIMH010000655         1639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3048227, whole genome shotgun sequence.
ACCESSION   DATIMH010000655 DATIMH010000000
VERSION     DATIMH010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3048227"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..667
                     /locus_tag="VLI90_11365"
     CDS             182..667
                     /locus_tag="VLI90_11365"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSV14852.1"
                     /translation="MTIKVGDKLPDAKFRVMTAEGPQVKTTDEIFKGKKVALFAVPGA
                     YTGTCHKMHLPSIFLNAYAIKDKGVNSIAIVSVNDAFVMNAWKRDTDQRDEAVFLADG
                     NAEFTKAIGMELDASGNGLGIRSKRYSMLVDDGVVKKLNLEPAPGKVEVSGGDTLLAQ
                     L"
     gene            complement(928..1392)
                     /gene="rnhA"
                     /locus_tag="VLI90_11370"
     CDS             complement(928..1392)
                     /gene="rnhA"
                     /locus_tag="VLI90_11370"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HSV14853.1"
                     /translation="MSSHPTVTIHTDGACSGNPGPGRWGAILKFGDVEKELKGGEAHT
                     TNNRMELMAAISALEALKKPCTVDLYTDSQYVRQGITGWINSWKKNGWRTADKKPVKN
                     VDLWQRLDASLHQHKVRWHWVKGHAGHDVNERADQLARDGVAMARLKTAIEK"
     gene            complement(1389..>1639)
                     /locus_tag="VLI90_11375"
     CDS             complement(1389..>1639)
                     /locus_tag="VLI90_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14854.1"
                     /translation="RAFLNAYGRERQLSTAEQEALPLLARGAALRILLTRLVDWFNVP
                     PGAMVKPKDPLEYVRKLRFQQGVASMSDYGVTASGLVA"
BASE COUNT          341 a          502 c          488 g          308 t
ORIGIN      
        1 ccaatgtcgg aaagatcctg cggcgcgagc tgcgcgacga aaagaaggcc gtggcggcct
       61 gagccgccga gccgaaacta gccggcgaac gcgcgacgga tgcccctttc cggcggcgag
      121 aacattgcgt cccatgcccg cggaaggtaa gaagcaccgc acagaataag gagagttgac
      181 gatgacgatc aaggttgggg acaagctgcc cgatgcaaaa ttccgcgtca tgacggcgga
      241 aggcccgcag gtgaaaacga cagacgaaat cttcaagggc aagaaggtcg cgctgttcgc
      301 ggtgcccggc gcctacacgg gcacttgcca caagatgcat ctgccgagca tctttctcaa
      361 tgcttacgcc atcaaggaca agggcgtgaa cagtatcgca atcgtctcgg tcaacgacgc
      421 ctttgtcatg aatgcgtgga agcgcgatac cgaccagcgc gacgaggctg tcttcctggc
      481 cgacggtaac gccgaattca cgaaggccat tgggatggag ctcgatgctt ccggcaatgg
      541 actcggcatc cgctcaaagc gctattcgat gctggtggat gacggcgtgg tgaagaagct
      601 caatctcgag ccggcgcccg gcaaggtcga agtttctggc ggcgatacgc tgctggcgca
      661 gctgtaaagc gttctaagct ccgcccaact ggctttcggc cagcgctccc tacgagtgga
      721 aacgacaaga gagaacgagc gtcattgcga cgagcgaacg cgacgaagca atccagtctt
      781 gccgttactc tatggattgc ttcgcttgcg ctcgcaatga cggcgcaaac ttgattgtgc
      841 gcgcagcgtc attgcgtatg tgcaaccgca tcgccggtct tcatccctcc ccacaaaggt
      901 gggagggata ggaagaggca atacccctca cttctcgatc gcggtcttca gccgcgccat
      961 tgcgacgcca tcacgggcta gctgatcggc gcgctcgttc acatcgtgac cggcgtgtcc
     1021 cttgacccag tgccagcgca ccttgtgctg gtgaagcgat gcgtccagcc gctgccagag
     1081 atcgacgttc ttgaccggct ttttatcggc ggtgcgccag ccatttttct tccagctatt
     1141 gatccagccg gtgatgcctt gccgcacata ctgactgtcg gtgtaaaggt cgaccgtgca
     1201 gggctttttc aaagcttcca gcgcggagat cgccgccatc agctccatgc ggttgttggt
     1261 ggtatgcgcc tcgccgccct tcaattcctt ttcgacgtcg ccgaacttca ggatcgcacc
     1321 ccagcgaccg ggaccgggat tacccgagca ggcgccatcg gtgtgaatcg tgacggtcgg
     1381 atgcgaactc acgccaccag ccccgaggcg gtgacaccgt aatcgctcat gctggcgacg
     1441 ccttgctgaa agcgcagctt gcggacgtat tccagcgggt ctttcggctt gaccatcgcg
     1501 cccggcggaa cattgaacca gtccaccagc cgggtcagca aaatccgcaa cgccgcccca
     1561 cgcgccagca atggcaacgc ctcctgctcg gccgtggaga gttgtcgctc acgaccatac
     1621 gcgttgagaa acgcccgtg
//
LOCUS       DATIMH010000656         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4489557, whole genome shotgun sequence.
ACCESSION   DATIMH010000656 DATIMH010000000
VERSION     DATIMH010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4489557"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..507
                     /locus_tag="VLI90_11380"
     CDS             70..507
                     /locus_tag="VLI90_11380"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSV14855.1"
                     /translation="MNNPLDSKLSQNLGLLLARVPLGAYLLMDGYFRIRNGVGAFASS
                     HQAATEHFLPGGVASAFLHAFPFLEVAAGAFLALGIFSRIGGFLASLVLITVVGAFTG
                     FAGAGNAPFNPFIILFGLAVFLCCAGAGGISVDRFVWGRRKGA"
     gene            627..1199
                     /locus_tag="VLI90_11385"
     CDS             627..1199
                     /locus_tag="VLI90_11385"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSV14856.1"
                     /translation="MDINPVVIILLVAVIIAICLPLAWYNKLIRLRQLIRESWGNVDV
                     QLKRRYDLIPNLVNTVKGYATHEQQTLTAVTEARNRAINNNGAPGSQAQDEQQLVRAT
                     RQLLAVAESYPDLKASQNFMQLQQELVNTEDRIAAARRFYNANVRDYNVCRTSFPWML
                     VAGLGDFPPQDFFEVDDYTVRQAPVVSMST"
     gene            1262..1873
                     /locus_tag="VLI90_11390"
     CDS             1262..1873
                     /locus_tag="VLI90_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14857.1"
                     /translation="MTMLLSAGCAAPHIRPPVNPPTPAVIYLCDYGVHSSILLPIGGG
                     RFIEYVYGDWAFAVENQTDPLHTLGALFVSFDPALGRRVLEPQPGRAYPWPPNGPHFV
                     RPMIVDAAKVQDVVNDLDARYRRHIDTAKLNDAPDYKFMFVKDDERYSIFHSCNHLTM
                     RNLKLMDCQIDGWTIGSNFLVDPPGERRPAGRGPSKFARPVEY"
BASE COUNT          345 a          589 c          606 g          377 t
ORIGIN      
        1 ctcccggagt accgggcgag ggggtcagag gcgcttcaac caataagttt gttcgatgga
       61 gtcatcgcta tgaacaaccc gctcgactca aagctgtcgc agaaccttgg cctcctgctg
      121 gcgcgcgtgc cgctcggcgc gtatctgctg atggatggct acttccgcat tcgcaacggc
      181 gttggcgcgt tcgcatcgtc gcaccaggcg gcgacggagc atttcctgcc cggcggtgtc
      241 gccagcgcgt tcctgcacgc gttcccgttt ttggaagtgg cggcgggcgc atttttggcg
      301 ctgggcatct tcagccgcat cggcgggttc ctcgcgtcac tggtgttgat caccgtcgtc
      361 ggcgcgttca ccggcttcgc cggcgcgggt aatgcgccgt tcaatccgtt catcatcctg
      421 ttcggcttgg cggtgttcct gtgctgcgct ggcgcgggcg ggatcagcgt cgaccgcttc
      481 gtctggggac ggcgaaaagg cgcgtaacgt tcgacatgcc gcttgcggct tagcgtccgt
      541 tcgacgtgaa aaaggacgct aagccgcaag cggccgtgtg acccggttga aactgcatcc
      601 cgttgcggcg aacatttgcc cgcagcgtgg acatcaaccc cgtcgtcatc atcctgctcg
      661 tcgcggtgat catcgcgatc tgcctgccgc tggcgtggta caacaagctg atccgcctgc
      721 ggcagctgat ccgcgaaagc tgggggaacg tcgatgttca gctcaagcgc cgatacgacc
      781 tgattcccaa tctggtcaac acggtcaaag gttacgcgac gcacgaacag caaacgctga
      841 ccgcggtgac cgaagcgcgc aatcgggcga tcaacaacaa tggcgcgccg gggtcgcaag
      901 cgcaggacga gcagcagctc gtgcgcgcga cgcggcaact gttggccgtt gccgagtctt
      961 atcctgatct gaaagcctcc cagaatttca tgcagctgca gcaggaattg gtgaacaccg
     1021 aggatcgcat cgccgccgct cggcggtttt acaacgccaa cgttcgcgac tacaacgtct
     1081 gccgaaccag ctttccgtgg atgctggtgg cgggcctggg ggactttccg ccgcaggatt
     1141 tcttcgaagt cgatgactac accgtgcggc aggcgccggt cgtgtcgatg agcacgtgat
     1201 tggccagatg aagtacacgc accgccgcat tcgcaccgct accgctgcga ctttcatttt
     1261 gatgacgatg ctgctcagcg ccggctgcgc cgcgccgcac attcgcccgc cggtgaatcc
     1321 accgacgccg gccgtcattt acctgtgcga ctacggcgtg cactccagca tcttgctgcc
     1381 gatcggcggc gggcggttca tcgaatacgt ctacggcgac tgggcgttcg cggtcgagaa
     1441 ccagaccgac ccgctgcaca cgctgggggc gttgttcgtg tcgttcgacc cggcgctggg
     1501 ccggcgggtt ctggaaccac aaccggggcg cgcatatcca tggccgccca acggcccgca
     1561 cttcgtccgg ccgatgatcg ttgatgcggc gaaggtccag gacgttgtga acgatctgga
     1621 cgcgcgctat cgccggcaca tcgacacggc gaagctgaac gatgcgccgg attacaaatt
     1681 catgttcgtg aaggacgatg agcgctacag catttttcac agctgcaatc acctgacgat
     1741 gcgcaatcta aagctgatgg attgccagat cgacggatgg acgatcgggt cgaattttct
     1801 cgtcgatcca ccgggggagc gacgaccggc aggcaggggg ccgtcgaagt tcgcaaggcc
     1861 ggtcgagtac tagatcgacg tgctggtcgg cccgttcagc atctgaagac acgatga
//
LOCUS       DATIMH010000657         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3625262, whole genome shotgun sequence.
ACCESSION   DATIMH010000657 DATIMH010000000
VERSION     DATIMH010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625262"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VLI90_11395"
     CDS             complement(<1..232)
                     /locus_tag="VLI90_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14858.1"
                     /translation="MFEKISYTWSLMRASWDVLKRDKGLLIFPLLSGISCLLIMASFA
                     VPIYQAKAWHPPGRDAQPVQQVMYYGALFCFYF"
     gene            complement(516..1850)
                     /gene="hslU"
                     /locus_tag="VLI90_11400"
     CDS             complement(516..1850)
                     /gene="hslU"
                     /locus_tag="VLI90_11400"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HSV14859.1"
                     /translation="MQTLTPKQIVAELDKYIVGQADAKRAVAIAIRNRWRRQQLPADI
                     RRDVSPKNILMIGPTGVGKTEIARRLAGLVGAPFLKIEATKYTEVGYHGRDVESMVRD
                     LVDVGIDLVRREQHETVKDSANEAVEDRLIDLLLPSLERVGPPEDDESKERRARTRER
                     FREQLRADELEDRPVEIVTEDKPDVNQLFGAAGMDMGFELQGMFEKMLPSKRETKRMT
                     VREARRFIASQEADKRVDNEGINRAAIERVEQSGIIFLDEIDKISGSEKSQGPDVSRQ
                     GVQRDLLPIVEGSTVTTKHGPVRSDHILFIAAGAFSQSKPSDLMPELLGRFPIRVELS
                     ELGKEDFVRILTEPQNSLIKQTVMLLATEGVKVDFTEGAIDAIASVAWRVNRKTQNIG
                     ARRLYTILERVFESISFDAPELGGKEVQITREYVKERLSTVAKDEDLSRYVL"
     gene            complement(2020..2093)
                     /locus_tag="VLI90_11405"
     tRNA            complement(2020..2093)
                     /locus_tag="VLI90_11405"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2057..2059),aa:Val,seq:cac)
     gene            complement(2603..>3042)
                     /locus_tag="VLI90_11410"
     CDS             complement(2603..>3042)
                     /locus_tag="VLI90_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14860.1"
                     /translation="AAANGKDDNLKFPVHLEHRVPVSTKTELDVADVQSSLRSVMWRN
                     VGIERSGEHLEETRDIIAFWSRYVMDKTFAADTIGPTAAVAGWELQNKLTACFIITTA
                     AYTRTESRGAHFRMDYPQRDDAHWRLHLLWKRPSETPLPEPIV"
BASE COUNT          548 a         1047 c          859 g          588 t
ORIGIN      
        1 cgaagtaaaa gcagaacaac gcgccgtagt acatgacctg ctgcaccggc tgcgcgtcgc
       61 gccccggcgg gtgccacgcc ttcgcctggt aaatcggcac cgcaaagctg gccatgatca
      121 gcaggcacga gatgcccgac agcagcggaa agatcagcaa gcccttgtcg cgcttcagca
      181 catcccacga cgcccgcatc aagctccacg tgtacgagat cttctcaaac atgaacgacc
      241 tcccttgcgt tgcgtaacaa caatgaaatg acccgcggcc caatcggccc atgccgcggc
      301 aagtatacct gattcagcga agtgatgtaa cacgggctgc gagccttcgg ctttctctcg
      361 cgcggcatca cctcgtccga atcgtcctaa ggaatcgagc gcatcggtgt gtggaacaac
      421 ttaaatagcc gggggcggaa gccccggtct gttcgcgagc aaaaagaccg gggctcccgc
      481 ccccggctat caacaggacc aatcactcgc ccgcgctaca gcacataacg gctcaaatcc
      541 tcatccttcg ccacggtcga cagccgctcc ttcacatact cgcgcgtgat ctgcacttct
      601 tttccaccca actccggcgc gtcgaagctg atcgactcga acacgcgttc cagaatcgtg
      661 tacagccggc gggcgccgat gttttgcgtc ttgcggttca cgcgccacgc cacagacgcg
      721 atcgcgtcga tcgcgccttc cgtgaaatcc accttcacgc cctcggtcgc cagcagcatc
      781 accgtctgct tgatcagcga attctgcggc tcggtcagga tccgcacgaa atcctccttg
      841 cccaactcac tcagctccac gcggatcgga aaccgcccga gcagttccgg catcagatcg
      901 ctcggtttgc tctggctgaa cgcgccggcg gcgatgaaca gaatgtgatc cgaccgcacc
      961 ggcccgtgct tcgtcgtcac cgtgctgcct tcgacgatcg gcagcagatc gcgctgcacg
     1021 ccttgccggc tcacgtccgg tccctgcgat ttttctgacc cgctgatctt gtcgatctcg
     1081 tccagaaaaa tgatcccgct ctgctcaacc cgctcgatcg ccgctcggtt aatgccttcg
     1141 ttgtcgaccc gcttgtccgc ctcctgcgat gcgatgaacc gccgggcttc gcgcacggtc
     1201 atgcgcttcg tctcgcgctt gctcggcagc atcttctcga acatgccctg cagctcgaac
     1261 cccatgtcca tgccggccgc cccgaacagc tggttaacat ccggcttgtc ctcggtcacg
     1321 atctcgacgg gccgatcttc caactcatcc gcgcgcagct gttcgcgaaa ccgctcgcgc
     1381 gtgcgcgccc ggcgttcctt cgattcgtca tcctccggcg gcccaacacg ttccagcgac
     1441 ggcaacagca gatcgatcaa ccgatcttcc acggcttcgt tcgcggaatc tttaacggtc
     1501 tcgtgctgct cccgccgcac caggtcgatg ccaacgtcaa ccagatcgcg caccatcgac
     1561 tcgacatccc gcccgtgata gccgacctcc gtgtacttcg tcgcctcgat cttgaggaac
     1621 ggcgccccaa ccagccccgc gagccgccgg gcgatttccg ttttccccac gccggtcggc
     1681 ccgatcatga gaatattttt cggcgaaaca tcgcggcgaa tatccgccgg cagctgctgc
     1741 cgccgccagc gattgcggat ggcgatcgct accgcgcgct tcgcgtcggc ctggccgacg
     1801 atgtacttgt ccaactccgc aacgatttgc ttaggcgtca acgtctgcat aggcggcgga
     1861 ttctagcgat cgcgcgccat ccgaccaccg ggacaaagat cgcgccgccc gcgctcagcg
     1921 cttagcgcgc agcgtccgcg atcttcgcgc agcggtatcc ccgcaggggg aactacctgg
     1981 ttgttgtggg gaagccgaag ccggcaggac ttatgaaaat gggcgatgag gggctcgaac
     2041 ccccgacctc acgggtgtga tccgtgcgct ctagccaact gagctaatcg cccgttcagt
     2101 gggttaatgt aaggcccctc cgccgcgaaa tcaacccgcg ctcttgggcg cagatggttt
     2161 ggaagtcgct tggcctttcg tggattcctc gtggggcttc cgaggacggg aagcgactgc
     2221 cgggcggttc gcacagttga ttctccaccg gttgccggca agatccttcg cctccggcga
     2281 agcatgcctg cgctggagga tgataccttg cgcgctcgca cggtcgccgg ggttgatcgg
     2341 cgtcggcgtg atcaacagcg ggatcgtgca attcaccggc ggcagcaccg tcgtcacgct
     2401 cttgcgaaac ttcaaccaca agaacagcct ggaggtcggc ccgggcgcgc aacggacgaa
     2461 ccaagcgaca ttcacgtcaa ccaacatgct ccacgtggct gggcagttca agagcaccgg
     2521 caccgcgatg atcggcggta cgcagttctg gggcgtccca tcgcagttca acaacaccgg
     2581 cgtcctcgcg tatacctgtc gcttaaacga tcggctccgg cagcggcgtc tcgctcgggc
     2641 gcttccacag caaatgcaac cgccagtgcg cgtcatcgcg ctgcggatag tccatgcgga
     2701 agtgtgcccc gcgcgattcg gtgcgtgtgt acgccgccgt ggtgatgatg aagcaggcgg
     2761 tgagcttgtt ctgcagttcc caccccgcga cggcggcggt ggggccgatc gtgtcggccg
     2821 cgaacgtctt gtccatcacg taccggctcc agaacgcgat gatgtcgcgc gtctcttcca
     2881 ggtgttcgcc gctgcgctcg atgccgacgt tccgccacat cacgctgcgc agcgagcttt
     2941 gcacgtcggc cacatccagc tccgtcttgg ttgatacggg cacgcgatgc tcgaggtgaa
     3001 cggggaactt caggttgtcg tctttcccat tcgccgccgc gt
//
LOCUS       DATIMH010000658         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3049050, whole genome shotgun sequence.
ACCESSION   DATIMH010000658 DATIMH010000000
VERSION     DATIMH010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3049050"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VLI90_11415"
     CDS             complement(<1..1502)
                     /locus_tag="VLI90_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HSV14861.1"
                     /translation="MTAIADPSARFVLPSDAPYLRNLAALWSVNATLAAEIEALEGQP
                     SYAVEPSKSGTPTMIVPTRDGRRVHLHSKHRPIDEAKRLVDPIDAQQGVFFNIHGLGL
                     GYTLELLFDRAGDEAVFCVFEPDLLLIRTALETRDLSRVIDSRRVHFFWQADKSDLFV
                     RLMPQTPMVTLGMHEVLHAASIQRDSEFHEQMQAWLAEFASFCKTNMNTLVMNGRRTL
                     ENIARNLGWYTATACPSRLKDRYKDKPALIVSAGPSLRKNKHLIQQMVGKSAIIAVQT
                     TLQPLLEMGVEPDFVTALDYHDICTRFFEKLPKGIKTELVAEPKATNLIFSLNPGPLT
                     VIGNDTAESLLREMRLNKTGLPSGSTVAHLAYYLAEHLGCNPIIFVGQDLGFSDGLYY
                     SPGTSYEDVWRPELGRFCTIEMKQWEQIVRERHILRRVPDHEGRPTYTEERLFTYLQQ
                     FERDFLSTKTKVIDSTEGGVLKRGAESMPFARAIAQYITEGAGVIERPSD"
     gene            1765..2346
                     /locus_tag="VLI90_11420"
     CDS             1765..2346
                     /locus_tag="VLI90_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14862.1"
                     /translation="MTDLLFSTPWWLPTAIIVAGVFLFVTGNARQLSGPRNVGLGLVG
                     LAIVLILVSWLVETDRERVTRQSRELIKSVETRDWNKMRSLLDPKVSLAISSATIYAN
                     RDDLVKGAQAATDQYGLKNIIITSLDAEQTQTVITVTLTALTEQDASMGRPVPSTWQF
                     DWEQIGKDWSLYRITCLRINNEQGPEMRGRFPK"
     gene            2367..2735
                     /locus_tag="VLI90_11425"
     CDS             2367..2735
                     /locus_tag="VLI90_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14863.1"
                     /translation="MSTHRYVILWHHDVGEPHYDLMFETYARSDLATWRSPRWPIEEA
                     VDLVRLKDHRRFYLEYVGDLSDRRGRVDRVAGGDCELEIGEGGVWDVRLINGGPPTRL
                     RIRQIEGERWVAEPVDGPVW"
     gene            3176..3493
                     /locus_tag="VLI90_11430"
     CDS             3176..3493
                     /locus_tag="VLI90_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14864.1"
                     /translation="MTRNSKLTIAAAALTGLLAGTASRAIAATTSSTPSSDTQTRKVV
                     QNGQKAVNLDDTDKHSCKGQNSCKGKGGCKTGDNGCKGKNSCKGKGGCKTEDGGDAGK
                     LLF"
BASE COUNT          679 a          974 c         1316 g          741 t
ORIGIN      
        1 tggtcggacg gacgctctat gacgccggcg ccttcggtga tgtactgcgc gatggcccgc
       61 gcgaacggca tcgactcggc gccgcgtttg agcacgccgc cttcggtgga gtcgatgacc
      121 ttcgtcttcg tcgagaggaa gtcccgctcg aattgctgga ggtaggtgaa gaggcgttcc
      181 tcggtgtacg tcgggcggcc ttcgtggtcc gggacgcggc ggaggatgtg gcgctcgcgg
      241 acgatctgtt cccactgctt catttcgatg gtgcagaacc ggccaagctc cggccgccag
      301 acgtcttcgt agctggtgcc gggggagtaa tagaggccgt cggagaaacc gaggtcctgg
      361 ccgacgaaga tgatcgggtt gcagccgagg tgctcggcca ggtagtacgc gaggtgcgcg
      421 acggtggacc cgctgggcag gccggtcttg ttcaggcgca tctcgcgcag cagcgactcg
      481 gcggtgtcgt tgccgatgac cgtcagtggt ccagggttca acgagaagat cagattggtc
      541 gccttgggtt cggcgacgag ttcggtcttg atgcccttcg gcagtttttc gaagaagcgc
      601 gtgcagatgt cgtgatagtc gagggcagtg acgaagtcgg gttcaacgcc catttccagc
      661 agcggctgca gcgtcgtctg caccgcgatg atggcggact tgccgaccat ctgctggatg
      721 aggtgcttgt tctttcgcag cgatggacca gcggagacga tcagcgcggg tttgtctttg
      781 tagcggtctt tcagccgcga agggcaagcg gtggcggtgt accagccgag atttcgcgcg
      841 atgttttcca gcgtgcgccg gccgttcatg accagcgtgt tcatgttggt tttgcagaag
      901 gaggcgaact cggcgagcca ggcttgcatc tgctcgtgga attcgctgtc gcgctggatg
      961 ctggcggcgt gcagcacctc gtgcatgccg agcgtgacca tcggcgtctg cggcatcagg
     1021 cggacgaaca ggtcggactt gtcggcctgc cagaagaagt gcacccggcg gctgtcgatg
     1081 acgcgcgaga ggtcgcgcgt ctcgagcgcg gtgcggatca gcagcaggtc gggctcgaag
     1141 acgcagaaga ccgcttcgtc gccggcgcgg tcgaagagca attcgagggt gtaacccagg
     1201 cccagtccgt ggatgttaaa gaagacgcct tgttgtgcgt cgatgggatc gaccagccgt
     1261 ttggcttcgt cgatcgggcg gtgcttgctg tgcaggtgga cgcgccggcc gtcgcgcgtc
     1321 ggcacgatca tcgtcggtgt gccggacttg gatggttcga ccgcgtaaga cggttggcct
     1381 tcgagggctt cgatctcggc ggcgagcgtg gcgttgacgg accagagggc ggcgaggttg
     1441 cgcaggtacg gcgcatcgct cggcaaaacg aaacgggcgg atggatcggc gattgcggtc
     1501 atcgtgcggc gcgcggtagc gttcgtggct gtagagaatt atcggacgtc gcacgcgcgg
     1561 gggttgagga aatctggttc tgcgaccacg ctgggttcgg aggcctgtcc tgagcgaagt
     1621 cgaaagatac tccgacgacc cggattgatt ggcgctgcca tcagacgaat ccgggtcgtc
     1681 gcgagtacgc tccgaacccg gcgtgggggg gaaatgtgtc gcgcgtggaa taggtcgcgc
     1741 gttcagtgat tttttgagga tttcatgacc gacctgctct tttcgacgcc gtggtggttg
     1801 ccgactgcga tcatcgtggc gggcgttttt ctgttcgtga cggggaacgc gcggcagttg
     1861 agtgggccgc ggaatgtggg attggggttg gtcgggttgg caatcgtctt gatcctggtg
     1921 agctggttgg tcgagacgga tcgcgagcgc gtgacgcggc agagtcggga gctgatcaag
     1981 tccgtcgaga cgcgcgattg gaacaagatg cggtcgctgc tggacccgaa ggtgagcctg
     2041 gcgatttcat cggcgacgat ctacgctaat cgggatgatc tggtgaaggg cgcgcaggcg
     2101 gcgacggatc aatatggtct gaagaacatc atcatcacct cgctggacgc cgagcagacg
     2161 cagacggtga tcacggtgac gctgacggcg ttgacggagc aggatgcttc gatgggccgg
     2221 ccggtgccga gcacctggca gtttgattgg gagcagatcg ggaaggattg gtcgctgtat
     2281 cggatcacgt gcttgcggat caacaacgag caagggccgg agatgcgcgg gcgatttccg
     2341 aagtagcgtt agcgggtgct aggtttatgt cgacgcatcg gtacgtcatc ttgtggcatc
     2401 atgatgtggg tgagccgcat tatgatttga tgtttgagac gtatgcgcgg tcggatttgg
     2461 cgacgtggcg gtcaccgcgg tggccgatcg aggaggcggt ggatctggtg cgactcaaag
     2521 atcatcgccg cttttatttg gagtatgtgg gggacttgtc ggatcggcgc gggcgcgtgg
     2581 atcgagtggc gggtggggat tgcgagttgg aaattggcga gggtggggtg tgggatgtgc
     2641 ggttgatcaa tggggggccg ccaacgcggt tgaggattcg gcagatcgag ggggaacggt
     2701 gggtggcgga accggtcgat gggccggttt ggtgagcgcg gtcaattcgt gcggcactgc
     2761 aaatcgaact gcaaatgcaa agtttgcctt gcaattgcga agcaacatcc gaaataatgt
     2821 ctgccgcgta atggttcaat gtgctgggac gatcacttca aacctctatg ccaacccata
     2881 ccaaggaggt gcaacaggaa ggatgacggg gccgcgccgc cttccacgcg ggcgccgggt
     2941 gcatcgttcg ccttggccac tgcgacgcta gcgcgcgtgt tgagaagttg aagcgtttcc
     3001 agaggtaccc gatgtaactc gcccggcccg gccggcatcg aaatgccacg ggccctggac
     3061 gagatcttct gtgcaaagcg actggcgcgt tgtgcgcggg tcgcggacgc agattcgcgg
     3121 cagatgtcga acccaccacg gacgacgctc cgcttcacaa cgaaagggac agatcatgac
     3181 gcgtaattca aagctcacca tcgcggccgc cgcgctgacc ggactgctcg ccggcaccgc
     3241 cagccgcgcg atcgccgcca cgaccagctc gaccccgtcg agcgacacgc agacgcgcaa
     3301 ggtcgtccag aacggccaga aggccgttaa cctggacgac accgacaagc actcctgcaa
     3361 gggtcaaaac tcgtgcaagg gcaaaggcgg ctgcaagacc ggcgataacg gttgtaaggg
     3421 aaagaattcg tgcaagggta agggcggctg caagaccgaa gacggcggtg acgccggcaa
     3481 actgctgttc tgatccatcc atcactcaaa cgcccaacga gagccggatc ggcccgtccg
     3541 gctctcgttg gttttgttcc gcacttcacc cggtttttct ctatgcccgc aaaccgcttc
     3601 aatggattta ccgagctcgg cgtcggcatt ggtctgcgcg tgccgcacta tcggcacatc
     3661 ctcagtaaaa agccgctcgt cgattggttc gagatcatca gcgaaaacta
//
LOCUS       DATIMH010000659         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5361331, whole genome shotgun sequence.
ACCESSION   DATIMH010000659 DATIMH010000000
VERSION     DATIMH010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5361331"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VLI90_11435"
     CDS             complement(<1..663)
                     /locus_tag="VLI90_11435"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSV14865.1"
                     /translation="MQVVLVMFRGDGERRSFSVVRDMTVIGRREDCDLRIPVGDVSRK
                     HCRLVMDGETLKVEDLGSSNGTYVNNQRVQEAWLTAGDVIQVGPVQFVVQVDGVPSDE
                     DLASPAAAAATEETAAGEAVLEEITDEPALDEVTLEEEPEQLPAEAELGEAELATGQL
                     AGPGEPTIDEPLDAVDLEEAPAEEPVLEEAEEASLEEVQLEEADELPLPEPPAPIVMP
                     EAE"
     gene            830..2008
                     /gene="hemW"
                     /locus_tag="VLI90_11440"
     CDS             830..2008
                     /gene="hemW"
                     /locus_tag="VLI90_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HSV14866.1"
                     /translation="MQSLTPRDLPAGVIEGLYVHIPFCFHKCHYCDFYSITRQSTERM
                     HTFVDRLLREAEQWQAARSVRPQTIFFGGGTPSLLPIDQMRRLIEGLHGRFDLSAVNE
                     WTVEANPATVTAEYCAMLREQGVDRLSFGAQSFRPSELATLERHHDPADVPRSIDIAL
                     AAGFTRLNVDLIFAIPGQDMASWSESLESAIALNTPHLSCYALTYEPNTPMAVRKRLG
                     HFTAVDEDIELAMLHHTRDRLIEIGRPPYEVSNFAAPGEACRHNVNYWTGGNYIGLGP
                     SAASHVQGWRWRNRPHLGEWEAAIDAGDLPATEIEVLTPSRRAGELAMLMLRLESGIN
                     FGQFNQRTGKDVRQLYRDVVERLARLELLDATSQTIRLTRAGINVADAVAAEFLEPTD
                     "
     gene            complement(1959..2480)
                     /locus_tag="VLI90_11445"
     CDS             complement(1959..2480)
                     /locus_tag="VLI90_11445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HSV14867.1"
                     /translation="MPLLRIVLDTTVLPGLVADESVATKILRAAGSAPILTSAQQAAH
                     GRISCRPSDAELVERFPHLTPRRVEVAMRRLRFIGEYIRSPNRRFALPRDPRDEKLIE
                     LAIALGATHIVSDDRDLLDLPTSRAAVGRRFRQLLPRVQVLSAGELLRSHAKWLKVNR
                     SAPGTPRRRHRQH"
     gene            complement(2487..2777)
                     /locus_tag="VLI90_11450"
     CDS             complement(2487..2777)
                     /locus_tag="VLI90_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14868.1"
                     /translation="MSSLASTIRISGLGKDKLTRLRAQAKACGVTAGRYAKQLIEDGL
                     SLDQQARSTTFDELMAPVRSGFRSSGMSQPKLAALVDRARTQHRVRFSGKHR"
     gene            complement(2847..3029)
                     /locus_tag="VLI90_11455"
     CDS             complement(2847..3029)
                     /locus_tag="VLI90_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14869.1"
                     /translation="MIWSSLFTVGNFLYGRYTPAVLLGITFAISAAVLIAVVRRIWAK
                     PAFIEGEAIEAAVVGI"
     gene            complement(3110..>3437)
                     /locus_tag="VLI90_11460"
     CDS             complement(3110..>3437)
                     /locus_tag="VLI90_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14870.1"
                     /translation="VKDTFDIILLVGAGTGLLYLVRWFWWRMNAWCEVVAMASAFLIP
                     VGWLALAKADVHVSTHVQLLLTIWRTTACWLVTAFLAPPTDPEVLVAFYRKVRPPGSG
                     GNAFAS"
BASE COUNT          672 a         1128 c         1027 g          610 t
ORIGIN      
        1 ttccgcctcc ggcatgacaa tcggcgccgg cggttcgggc agcggcaact cgtccgcttc
       61 ttccagctgc acttcttcta atgacgcctc ttcggcttct tcaagcaccg gttcttccgc
      121 gggcgcttcc tctaaatcga ccgcatccag cggttcatca atcgtcggtt cgcccgggcc
      181 ggctaactga ccggtggcca attccgcttc acccaactcc gcctcagcag gcaattgctc
      241 gggctcctct tccagcgtca cctcgtccag cgcgggttcg tcggtgattt cttccagcac
      301 cgcttcgccg gccgccgtct cttccgttgc cgcggcagcg gcgggcgagg ccaggtcttc
      361 atccgatggc acgccgtcca cctgcacgac gaactgcacc ggaccgacct ggatcacatc
      421 accggcggtc agccaggctt cctgcacgcg ctggttgttg acgtaagtgc cgttggaact
      481 gcccaggtct tcgaccttca gcgtttcgcc atccatgacc agccggcagt gcttgcgcga
      541 gacatcgccg acgggaatgc gcagatcgca gtcctcgcgc cggccgatca ccgtcatgtc
      601 gcgcacgacg gagaagcttc ggcgttcgcc gtcgccgcga aacatcacca acaccacctg
      661 catagcgcgt gcctcaaaaa agtgatggga aacgaggcag atcaacagga caacttagag
      721 acaatgccca agagcaagcg tagcgtagaa tggaaccgcc agcaaggtta aactcccccg
      781 gcagcgggat gcgaaatcga tgcaactgtc gttattaccg gattcttctg tgcaatctct
      841 caccccgcgc gacctgccgg cgggggtgat cgaggggctc tacgtccaca tccccttctg
      901 cttccacaag tgccattact gcgattttta cagcatcacc cggcaatcga ccgagcgcat
      961 gcacaccttc gtcgatcggc tgctgcgcga ggccgagcaa tggcaagcgg cccgaagcgt
     1021 tcggccgcag acgatcttct tcggtggcgg cacgccgagc ctgctgccga tcgaccagat
     1081 gcggcggttg atcgaaggac tgcacgggcg gttcgacctg agcgcggtga acgagtggac
     1141 cgtcgaggcg aacccagcga cggtgacggc ggagtactgc gcgatgctgc gcgagcaagg
     1201 cgtggaccgc ctgagcttcg gcgcgcaaag ttttcgacca tcagaattgg cgacgctcga
     1261 gcggcatcac gatccggcgg acgtgccgcg cagcatcgac atcgcgctgg ccgcggggtt
     1321 cacgcggttg aatgtcgatc tgatcttcgc gatccccggg caggacatgg catcgtggtc
     1381 cgagtcgctc gaaagcgcaa ttgcgctcaa cacaccgcac ttgtcatgct acgcgctgac
     1441 atacgaaccc aacacaccga tggccgtgcg aaagcgcctc ggtcatttca cggccgttga
     1501 tgaagacatc gaactggcga tgctgcacca cacgcgcgac cggttgatcg aaatcggtcg
     1561 accgccgtac gaggtgagca acttcgccgc ccctggcgaa gcgtgccggc acaacgtgaa
     1621 ctactggacc ggggggaact atattggctt gggaccgtcg gccgcgtcgc atgtccaagg
     1681 ctggcgctgg cgcaatcgcc cgcacctggg cgaatgggaa gccgccatcg acgccggcga
     1741 tttgcccgcg acggagatcg aggtgctcac gccctctcgt cgcgccggcg aacttgcgat
     1801 gctgatgctg cggcttgaga gcgggatcaa ctttgggcag ttcaaccagc gcacgggcaa
     1861 agacgtccgc cagctctatc gcgacgtggt cgaacggctt gcacgactcg aacttctgga
     1921 cgcgaccagc cagaccattc gtctcacccg cgccggtatc aatgttgccg atgccgtcgc
     1981 cgcggagttc ctggagccga ccgattaacc ttcagccatt tcgcatggct ccgcagcaac
     2041 tcgccggcgg acagcacctg aacacggggc aggagttgtc tgaacctgcg gcccacggct
     2101 gcgcggctgg ttggtagatc cagcaagtcc cggtcatcgc tgacaatgtg tgtcgcgccg
     2161 agcgcgatcg ccagttcgat gagcttttca tcacgaggat cgcgaggaag cgcgaagcga
     2221 cggttggggc tgcgaatgta ttcaccgatg aagcgcagtc ttcgcatcgc cacctcaaca
     2281 cgccgcggtg tcagatgcgg gaaacgttcg accaattccg catcggaagg acggcacgat
     2341 attcgtccat gagcggcttg ctgagcagag gtaagaatcg gcgcgcttcc cgctgcccgc
     2401 aagattttcg tcgccacgga ttcatcggcg acgaggcctg gcaataccgt cgtatccagg
     2461 acaatgcgga gcagaggcat gggcacttat cggtgcttcc ccgaaaaccg cacacggtgt
     2521 tgcgtccgcg cgcggtcaac cagagccgcc aattttggct ggctcatacc actggaacga
     2581 aaacccgacc gcaccggcgc catcaactcg tcaaaagttg tgctccgcgc ctgttgatcc
     2641 agggacagtc cgtcttcaat cagctgcttt gcgtaacgcc cggccgtgac gccacaggcc
     2701 ttcgcctgtg cgcgcaatcg cgtgagcttg tccttgccta gcccggaaat gcgaatggtc
     2761 gacgcgagtg aactcatacg tttgccgcct cgatcattct accaaatgtg cgcgtccaac
     2821 gcggcgttga atgccaatgc caattatcag attccaacca cggcggcctc gatggcctca
     2881 ccctcgatga acgccggctt cgcccaaatg cgccggacca ccgcgatcag caccgccgcg
     2941 ctgatggcga acgtgatgcc cagcaatacc gcgggcgtgt agcgaccgta aaggaagttg
     3001 ccgaccgtga agaggctcga ccagatcatc gcgcagccgg cgacccagcc taccagcgcc
     3061 agcggcaggt tctccgacgt tgcgcgggcc tcgttcttcg agatgccgct cagctggcga
     3121 atgcgttccc acccgaaccc ggcgggcgga ccttgcgata gaacgcgacg agcacctcgg
     3181 ggtccgtcgg cggggcgagg aacgcggtga cgagccagca cgcggtcgtt cgccagatcg
     3241 tcagcagcag ctgaacatgg gtcgaaacat gaacgtcggc cttggccagc gcgagccagc
     3301 cgacgggaat caggaacgcg ctggccatcg cgacgacttc gcaccacgcg ttcatgcgcc
     3361 accagaacca tcggaccagg tacagcaacc cggtgccggc gcctaccagc aggatgatgt
     3421 cgaacgtgtc cttcaca
//
LOCUS       DATIMH010000660         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2468659, whole genome shotgun sequence.
ACCESSION   DATIMH010000660 DATIMH010000000
VERSION     DATIMH010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468659"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VLI90_11465"
     CDS             complement(<1..749)
                     /locus_tag="VLI90_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HSV14871.1"
                     /translation="MPAQIIDGAALAARMKQEVAAKVTALASRGKAVHLTAILAGSTP
                     AGELYAQRQGEACRAVGINYQLLTLPEQIAQRDLKKEIRKLNTDPSVTGIMMHLPLPA
                     HLDRARLQYEIDVVKDVEGVNPANIGYVVYGRTLIAPCTALSVIELIKSTGVKLEGAE
                     AVIVGASEIAGKPIALLLAEQMATTTLCHIATRDVKSHTTRADIVVVAVGKPGLIGAD
                     HVRDGAIVIDVGINRVAAADGTKKTVGDVDF"
     gene            complement(894..>3217)
                     /locus_tag="VLI90_11470"
     CDS             complement(894..>3217)
                     /locus_tag="VLI90_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14872.1"
                     /translation="QRALPQGTEAIVISKLEYRGKPAWVIAGSDERGLMYGELDVADR
                     IGWSADPQQPLSEVRDTAEQAATTTRAVSIYTMNRAYWESRFYDEAYWTRYLDTLARN
                     RFNSLVVIFGYENGGFLAPCYPYFFDTDGFEGVRMVGITPAEQARNVKALNRLIQMSH
                     DRGIRFTVGIWDHIYRGGVQGGGVPGADEAVRHPTPGLVWGVTGDNLVPYTKAALAKF
                     IRAVPDLDGIQFRMHDESGLKRGEQEGFWRDVFEMIKQTAPRLRLDLRAKGLPDSVIQ
                     SALDVGVNFRITTKYWMEQMGLPFHPTHVNPQDQRNRRHGYADLLKYPQQYTMHWRLW
                     NGGPSRVLLWGDPEYARRFVQSTHLYDGDGFEINEPLATKMEGQPHDAKPFELMKEPY
                     RYYDYEFERYWHLFQVFGRLGYNPDTPTEVWHHEFEQRFGPDAGPVVEAALHQASWIL
                     PRIVASSYPYSAFPMTAGWAEKQHLGNLPAFAKSQGSDVAQFENFDEEAQRLIDSGET
                     AKTRPQDTSRWFQQAGDSVKKLVAKAEQHVGSHRNREFDSTMVDLKMLSNLALYHARR
                     IPAAVSYRLYVRTKDPAALDDAIAGERSAIDAWRNIVAAAGDVYADDLEMGARSRNLC
                     GHWRDELAAMEKDLPALEQERAQASPATRASPRYAAIPAPPPETIVVIHAPPTSASPG
                     KPLTITADVHGPAGIKWVRLRYRPVNQYLDYDTLPMSSSGIDHYEATVPGEKIVSTWD
                     FMYYIEVMDNAGHGQIYPDFNDTSPYVVVHLTR"
BASE COUNT          526 a         1082 c          981 g          628 t
ORIGIN      
        1 tcgaaatcca catcccccac cgtcttcttc gtgccgtcgg ccgccgccac gcggttgatg
       61 ccgacgtcga ttacgatcgc gccgtcgcgc acgtggtcgg ccccgatcaa cccgggtttg
      121 cccacggcga cgacaacgat atccgcccgc gtcgtgtgcg acttcacgtc gcgcgtcgcg
      181 atgtggcaca gggtggtcgt cgccatctgc tcggccaaca acagcgcgat cggtttgccg
      241 gcgatctcgc tggcgcccac gatgaccgcc tcggcgcctt ccaacttcac gccagtggat
      301 ttgatcaatt cgatcacgct gagcgctgtg cacggcgcga tgagcgttcg gccatagacg
      361 acgtacccga tgttcgcggg gttcacgcct tcgacgtctt tgacgacgtc gatctcatac
      421 tgcaatcgcg cccggtccag gtgagccggc agcggcaggt gcatcatgat gccggtgacg
      481 ctcgggtcgg tgttcagctt gcggatctct tttttcaaat cgcgctgcgc aatctgttcc
      541 ggcagcgtga gcaactgata gttgatgccg actgcccggc acgcttcgcc ctgccgctgc
      601 gcgtatagct cgccggcggg agttgaaccg gcgaggatgg ccgtcaggtg caccgccttc
      661 ccgcgcgacg ccaacgccgt cactttggcg gcgacctcct gcttcatacg agcggccaac
      721 gccgccccgt cgatgatctg tgcgggcata gggtgtgagt atagccaatt caggcgcgac
      781 cgctgcgctc acgccgggtt cggaggtact ccggcgaccc ggattctgcc gcgcgtcaag
      841 cagaagagat ccgggttgtc ggggtacctc cgaacccggc gtggtgagag aacctatcgc
      901 gtcagatgca ccacgacata cggcgaagtg tcattgaagt ccggatagat ctgcccatgc
      961 cccgcgttgt ccatgacctc gatgtaatac atgaagtccc acgtcgaaac gatcttctct
     1021 cccggcacgg tcgcctcgta atgatcgatg cccgaactgc tcatcggcag cgtgtcgtaa
     1081 tcgagatatt ggttcaccgg ccgatagcgc agccgcaccc acttgatgcc cgccggcccg
     1141 tggacgtcgg cggtgatcgt cagcggcttg cccggtgatg ccgatgtggg cggcgcgtga
     1201 atgacgacaa tcgtctccgg cggcggcgcg ggaatcgccg catagcgcgg tgacgcccgc
     1261 gtcgcgggcg acgcctgtgc ccgctcctgc tccagcgcgg gcaggtcctt ttccatcgcc
     1321 gccagttcgt cgcgccagtg gccgcacagg ttccggcttc gcgcgcccat ctccagatcg
     1381 tccgcgtaca catcgcccgc ggcggcgacg atgtttcgcc aggcatcgat ggcgctgcgt
     1441 tccccggcaa tcgcatcatc gagggccgca ggatctttgg tgcggacgta cagccgatag
     1501 ctcaccgccg ctggtattcg ccgggcgtga tacagcgcga ggttcgacaa catcttcagg
     1561 tcgaccattg tcgaatcgaa ctcgcggttg cgatgcgagc caacgtgctg ctcggccttg
     1621 gccaccagct ttttcacgct atcgccggcc tgctgaaacc atcggctcgt atcctgcggg
     1681 cgggtcttcg ccgtctcgcc gctgtcgatc agccgctgcg cttcctcgtc gaagttttcg
     1741 aactgcgcaa cgtcgctgcc ctgcgacttg gcgaacgccg gcaggttgcc caggtgctgc
     1801 ttctccgccc acccggccgt cataggaaac gcgctgtagg gataactcga cgcgacgatc
     1861 cgcggcagga tccagctggc ttggtgcaac gctgcttcaa cgaccggccc ggcatcgggg
     1921 ccgaagcgct gctcgaactc gtgatgccac acctcggtcg gcgtgtccgg attgtaaccc
     1981 agccggccaa acacctggaa caggtgccaa tatcgctcga actcgtagtc gtaataccgg
     2041 tacggctcct tcatcaactc aaacggcttg gcatcgtgcg gctggccttc catcttcgtc
     2101 gccagcggtt cgttgatctc gaaaccgtcg ccatcgtaca ggtgcgtcga ctgcacgaat
     2161 cgccgggcgt attccgggtc gccccacagc agcacgcgcg acggcccgcc attccacagc
     2221 cgccagtgca tcgtgtattg ctgcggatac ttcagcaggt cggcgtaacc gtgccggcgg
     2281 ttgcgctggt cctgcgggtt cacgtgcgtc ggatggaacg gcaggcccat ttgctccatc
     2341 cagtatttcg tggtgatgcg gaagttcacg cccacgtcga gcgccgattg gatgaccgaa
     2401 tcgggcaatc ctttggcgcg cagatcgagc cgcaggcgcg gcgcggtctg cttgatcatc
     2461 tcgaacacgt cgcgccagaa accttcctgt tcgccgcgtt tcaggccgga ttcatcgtgc
     2521 atgcggaact gaatgccatc gaggtccggc actgctctga tgaatttcgc gagcgccgcc
     2581 ttcgtgtacg gcaccaggtt gtcaccggtc acgccccaca ctaatcccgg cgtcgggtgg
     2641 cgcaccgcct catccgcgcc cggcaccccg ccgccttgca cgccgccgcg atagatgtgg
     2701 tcccagatgc cgacggtgaa gcggatgccg cggtcgtgcg acatctgaat caggcgattg
     2761 agcgccttca cattccgcgc ctgctctgcc ggcgtgatgc cgaccatgcg cacgccttcg
     2821 aaaccgtcgg tgtcgaagaa gtacggatag cacggcgcga gaaatccgcc gttctcgtag
     2881 ccgaagatca ccaccagcga gttgaagcga ttgcgggcga gcgtgtcgag atagcgcgtc
     2941 cagtacgcct cgtcgtagaa ccggctctcc cagtacgcgc ggttcatcgt gtagatcgac
     3001 accgcccgcg tggtcgtggc ggcctgctcg gccgtgtcgc gcacttcact taaaggctgc
     3061 tgcggatcag cgctccagcc gatgcgatcg gccacgtcca actcgccgta catcaacccg
     3121 cgctcgtcgg acccggcgat cacccacgcc ggcttgccgc ggtattccag cttgctgatg
     3181 acgatggctt cggtgccctg cggcaacgcg cgctggt
//
LOCUS       DATIMH010000661         3381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3049572, whole genome shotgun sequence.
ACCESSION   DATIMH010000661 DATIMH010000000
VERSION     DATIMH010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3049572"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VLI90_11475"
     CDS             complement(<1..584)
                     /locus_tag="VLI90_11475"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14873.1"
                     /translation="MSEPVAERLRQLIAAVPDPAAARHFLDCLRQDAPAGYERILHSP
                     AALRCAISVFSFSRFLSESVVRDPERLLLVANSGTFYRALSVDDYRDRLLHFLGPRPS
                     AIDFARFRRRHLLRILLRDVLGVATLGDVTDELSHLADAILDVAYRRIRADLVAQHGE
                     PRLEDRTPCGFSVISLGKLGGRELNYSSDIDLMFV"
     gene            complement(590..1084)
                     /locus_tag="VLI90_11480"
     CDS             complement(590..1084)
                     /locus_tag="VLI90_11480"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HSV14874.1"
                     /translation="MEASVVQVNISPGGLPKRAIDEGIIGPLGIEGDVHAHPQIHGGP
                     LQAVLLIAAEVVDALIERGYPLFYGAMGENVTTRGIAIRDLRIGDQLRVGGAVLEITK
                     PRGPCSALDIYGESLKRDVYDAQVKRRDPASPKWGMSGLYASVVRPGPVRAGDPVALL
                     STLA"
     gene            complement(1104..2495)
                     /gene="glnA"
                     /locus_tag="VLI90_11485"
     CDS             complement(1104..2495)
                     /gene="glnA"
                     /locus_tag="VLI90_11485"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HSV14875.1"
                     /translation="MEFAKKNDVKQLDLRFTDIPSLQHHVSYPISQLDENSFEEGFGM
                     DGSSIRGWAAIHESDMLLVPDPTTAVLDPFAEFPTLIMYGDIKDPITKQRYERDPRWI
                     AQKAELYLRNSGVADTAFVGAEAEFFIFDNISFDQNQHSGFYFIDAEEGRWNSSRREN
                     NLGYRPRYKEGYFPVPPTDHYQDLRSEMVQVMIKCGLEIECHHHEVATGGQCEIDQKY
                     DTLVKSADNMMLYKYIIRNVAYQYGKTVTFMPKPIFGDNGSGMHTHQSLWKDGKPLFA
                     GESYAGLSQMALHYIGGLLRHARALSAIIAPTTNSYKRLVPGYEAPVNLAYSRRNRSA
                     AVRIPMYSASPKAKRVEFRPPDPSANPYLAFAAMLMAGLDGVLNRINPGEPLDKDIYD
                     LSPEEMKSVPSMPGSLDEALSCLEDDHAFLMRGDVFTEELIETFIDYKRKHEAEAVRL
                     RPHPYEFALYYDI"
     gene            complement(2607..>3381)
                     /locus_tag="VLI90_11490"
     CDS             complement(2607..>3381)
                     /locus_tag="VLI90_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14876.1"
                     /translation="VSVKIPGSVVALAAKSGGAGETQFDFLAQIQDERRNVVGNVRDY
                     IKVKIDAADTEKMARRSFHYDAGFTLGPGRYRMKFLARENRSGKMGTFESRFTVPDLA
                     ADSAMLKTSSVIWSNQREALKAAVGAAEKATKKTVISNPLISDDQKVVPSVTKVFRRS
                     QNLYITFDVYDARPDPENAKARKVNVSMSLFNAKGMKTFEAGPIAATDLASTRPNAVP
                     VQMQVPLKGVAPGRYICQINVVDEVGRKFAFPRTAMVVQ"
BASE COUNT          608 a         1116 c          991 g          666 t
ORIGIN      
        1 acgaacatca ggtcgatgtc ggagctgtag ttgagctcac gcccgcccag cttgcccagc
       61 gagatcacgc tgaagccgca cggggtccgg tcctccagtc gcggctcgcc gtgctgcgcc
      121 accaggtcgg cccggatccg gcgatacgct acgtcgagta tcgcgtcggc aaggtgcgaa
      181 agctcgtcgg tgacatcgcc cagggtggcc acgccgagca catcccgcag cagaatgcgc
      241 aagaggtggc gccgccgaaa ccgcgcaaaa tcgatcgccg agggccgcgg tcccagaaag
      301 tgcagcagac gatcgcgata gtcgtccacc gaaagcgcgc ggtagaacgt tcccgagttc
      361 gctaccagca gcagccgctc gggatcgcgc accaccgatt cggaaaggaa ccggctaaac
      421 gagaacacgc tgatcgcgca ccgcaacgct gccggcgaat gcagaattcg ctcatatccc
      481 gcgggcgcgt cctgacgcag gcagtccaga aaatgacgcg ccgcggccgg atccggcacg
      541 gctgcgatca gttgccgcag tcgttccgca accggctcac tcaaagactt catgccagcg
      601 tgctgagcaa cgccaccgga tcgcccgccc gcaccggacc aggccgcacc acgctggcgt
      661 agaggccgct catgccccac ttcggcgacg ccggatcgcg gcgcttgact tgggcgtcgt
      721 acacgtcgcg cttcagcgat tcgccgtaaa tatccagcgc ggagcacggc ccccgcggct
      781 tcgtaatctc gagcaccgcc ccgccgacgc gaagctgatc gccaatgcgc aaatcgcgga
      841 tcgcgatgcc gcgcgttgtg acgttctcgc ccatggctcc gtagaacagc gggtagcccc
      901 gttcgatcag cgcgtccacg acttcggctg cgatgaggag caccgcctgc agcggtccgc
      961 cgtggatctg cgggtgcgca tgaacgtctc cctcgatgcc cagcggtccg atgatgccct
     1021 cgtcaatcgc ccgcttgggc agtcctcccg gcgatatatt gacctgaacg acggatgctt
     1081 ccatgagacg atcgtgccct ctattagatg tcgtagtaca acgcgaactc gtacggatgc
     1141 ggccgcagac gcaccgcttc cgcctcgtgc ttgcgcttgt agtcgatgaa ggtttcgatc
     1201 agttcttccg tgaacacgtc gcctctcatc aggaacgcat gatcgtcctc caggcagctc
     1261 agcgcctcgt cgagcgaccc cggcatggag ggcaccgact tcatctcttc cggcgataga
     1321 tcgtagatat ccttgtccaa cggctcgccg ggattgatgc ggttgaggac gccgtccagc
     1381 cctgccatca gcatggcggc gaacgcgaga tacggattgg ccgagggatc cggaggccgg
     1441 aactccaccc gctttgcctt cggactcgcc gagtacatcg ggatgcgaac cgccgcgctg
     1501 cggttgcgcc gcgaatacgc cagattgacc ggcgcttcgt agcccggcac caggcgcttg
     1561 tagctgttcg tggtgggtgc aatgatcgcg ctcagcgcac gcgcgtgacg cagcaggccg
     1621 ccgatatagt gcagcgccat ctggctcagc ccggcgtacg actctcccgc gaacagcggc
     1681 ttcccgtcct tccacaggct ctggtgcgta tgcatgccgc tgccgttatc gccgaaaatc
     1741 ggcttcggca tgaaggtcac cgtcttgccg tactgatacg ccacgttgcg gatgatgtat
     1801 ttgtacagca tcatgttgtc cgcgctcttg accagcgtgt cgtacttctg atcgatctcg
     1861 cattgccctc cggtcgcgac ctcgtgatgg tgacactcga tctccagccc gcacttgatc
     1921 atcacctgca ccatctcgct gcgcaggtcc tggtagtgat cggtgggcgg caccgggaag
     1981 tagccttcct tgtagcgcgg acggtagccc aggttgttct cgcggcggct ggaattccag
     2041 cgcccttcct cggcatcgat gaagtaaaag ccggagtgct gattctggtc gaagctgatg
     2101 ttgtcgaaaa tgaaaaactc ggcctcggcc ccgacgaaag ccgtatcggc cacacccgaa
     2161 ttgcgcaggt acagctcggc cttctgggca atccatcgcg gatcgcgttc gtagcgctgc
     2221 tttgtgatcg gatccttgat gtcgccgtac atgatcagcg tgggaaactc ggcgaacggg
     2281 tccaggacgg cggtcgtcgg atcgggtacc agcagcatgt cgctctcgtg aatcgcagcc
     2341 cagccgcgaa tgctggagcc gtccatgcca aatccttcct cgaagctatt ctcgtcgagt
     2401 tgcgaaatag ggtaagacac gtgatgctgc agactgggaa tatccgtgaa ccggagatcg
     2461 agctgcttca catcgttctt tttcgcgaat tccaaaactt cctgtggcgt catcggtgct
     2521 ccttcgcttc cgcgtcgatc caacgttcca gtatattcgg tagcgtccgc ggtgcagccg
     2581 ggcgaagttg gccgaagcga agccgcctac tggaccacca tcgctgtccg cggaaatgca
     2641 aacttgcgac cgacctcgtc cactacattg atctggcaga tgtaacgccc gggcgccacc
     2701 cccttcagcg gcacctgcat ctgcaccggg accgcattgg gacgcgtcga ggccagatcg
     2761 gtggccgcaa tgggacccgc ttcgaaggtc ttcatgcctt tcgcgttgaa caggctcatg
     2821 ctcacgttca ccttgcgcgc cttggcattc tccggatccg gccgcgcgtc gtagacgtcg
     2881 aacgtaatgt acaggttctg gctgcggcgg aacaccttcg tcacgctggg cactaccttc
     2941 tgatcgtccg agatcaacgg attcgagatc accgtcttct tggtcgcctt ctccgcggcg
     3001 ccgacagcag ccttcagcgc ttcccgttga ttgctccaga tcacgctgct ggtcttcagc
     3061 atcgcgctgt ccgccgccag gtccggcacg gtgaatctgg attcgaacgt gcccatcttc
     3121 cccgaccggt tctcgcgcgc cagaaacttc atccggtagc gccctggccc caacgtgaat
     3181 ccggcgtcgt agtggaaact gcggcgcgcc attttctccg tgtcggcggc gtcgatcttc
     3241 actttgatgt agtcgcggac gttccccacc acgttgcgcc gttcgtcctg aatttgcgcc
     3301 agaaaatcga attgcgtctc gcccgcgccg ccgctctttg ccgccagtgc gacgacgctg
     3361 cccgggatct tcacactgac c
//
LOCUS       DATIMH010000662         4675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1319524, whole genome shotgun sequence.
ACCESSION   DATIMH010000662 DATIMH010000000
VERSION     DATIMH010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319524"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VLI90_11495"
     CDS             <1..1549
                     /locus_tag="VLI90_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSV14877.1"
                     /translation="HANREDILQAARSLGARNVQPDAVSELVQEKLVSALRTVAATKD
                     LVELHSKRDEFASSVQQIVVHDLAHNGLTLESVTISALDQTDPSQLQERNVFDAQGLR
                     KIAEITQRARVERNEIEREAEKQVQEKNVSTTKTVLDLQRDQAEFEAEQKMKVANVRA
                     AREREVAEFKLIQDEAISRRDIEKMKNIETSEVERTLAVEQAQVAKQIALIAKVREQQ
                     TAEIMKKQTVEVAERNKEVAVAEKERERASAQAEVLTAEAEREAAKQKVLTVTVSSEA
                     DREAQKKLIAAQQLINENKIREETAADVSAYMAVKKAEAERQAAEAIYQAKLRLAEGD
                     SIAASKRADGEKAIKMVDINVERERVNVEQARVEVERQSLANKSEFEEAALKFELEKL
                     RIDAEREVRIQAAQAMGNMLSKANMQIFADPDTMARMQGQFMRAASLGTAADGLLKSL
                     PPQSQDVLAKLASAVASQLSPKAAAAVAEQTGNGNGATPIGVPVATDVAPAAVDSKPG
                     KKDHRMS"
     gene            1631..2047
                     /locus_tag="VLI90_11500"
     CDS             1631..2047
                     /locus_tag="VLI90_11500"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HSV14878.1"
                     /translation="MTWKDDLNYGLAAFGIQIPTDVPLDQWATKLTGQPARNTAAVVA
                     ASAVLFYIAERDYNSKVNDIWDACVYCSTCLSVGYGDIFAKTPAGKIIGTTLMTLGPA
                     LSGAALDGPAPPQLNDDVQAEVLATLKAILDRLPEK"
     gene            2383..>4675
                     /locus_tag="VLI90_11505"
     CDS             2383..>4675
                     /locus_tag="VLI90_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14879.1"
                     /translation="MTLRGVRAIRLAALAAAVSFAGPLAPASHADPINQIATWTGGAG
                     DGKWSSLANWSPQTVPANDANDLYNVIVGNNVNLTFDLPTAATINGISFGPGGSLAVG
                     SGDQLTITNVASLNSSVSSNGAGSIFTATGVGVSLGPNAHLAATAGGVINVAPSTFVY
                     TTGGIQVPVCTADGAGSVVNESTMTSMSANNSLTSSDIQPVYTATNGGAINLSGLLSI
                     DGGIGPHFMNIFRDVAVSETYFVIGANSDINLANLKQITGFAQFTVQRSSYSLPSLQT
                     ASATTFNLSAGTAMNLPALVTLAGGTSALNVPNTASFNAPVATTLNDTQFNVSAGGTV
                     NAHNVTTLDNSTLAIAVGGTVDIHSLQEATNTFIAMAPGANLDYTAPIRFDNSRLSIT
                     GGASFNVAATSYTYTRNASETAWSADGAGSVISAPSMTSMVSTQSFIGDVRPVYSATN
                     GGVINLSGLQTFNGGAGPLNQVAVGSSFSFSTFFIDSSSNIKLDSLKTLSGWNSFTIQ
                     RSSYSFPSLQTMSATTFTLNVGTTVNAPALTTVAGGSSSIPIPSFASFNASSLKTLND
                     TTLSLSVGGAFTAPQLSTIQNTNITFPSGADFTYGTIKNFDDSTVSISGGAVFNLAVP
                     SYTFDRDSTVTAWHTDGPGTRINSPSTSSIVDTVGFPAYRAINSSMIDLSGLRFALGA
                     NTFQANSGGSILFGSLKSYSNFTADGSASSLNFKSLNLTAGGTLVATNLANIVLQGSL
                     AYTTTSPSSINLDQGVLDFAADSR"
BASE COUNT         1003 a         1444 c         1449 g          779 t
ORIGIN      
        1 gcacgcgaat cgcgaagaca tcctgcaagc cgcgcgatca ctcggcgcgc gaaacgtgca
       61 gcccgatgcg gtgagcgagc tggtgcaaga gaagctggtc agcgcgctgc gcacggtggc
      121 ggcgacgaag gacctggtcg agctgcactc caagcgcgat gagttcgcca gttcggtgca
      181 gcagatcgtc gtgcacgatt tggctcacaa cggcttgacg ctggaatcgg tgacgatttc
      241 cgcgctggac cagacggatc cttcgcagtt gcaggagcgg aacgtgttcg acgcgcaggg
      301 cctgcggaag atcgcggaga tcacgcagcg cgcccgggtg gagcgcaacg agatcgagcg
      361 cgaggcggaa aagcaggtgc aggagaagaa tgtttcgacg accaagacgg tgctggacct
      421 gcagcgcgac caggcggagt tcgaggccga gcagaagatg aaagtcgcca atgttcgcgc
      481 cgctcgagag cgcgaggtgg cggaattcaa gctgattcag gacgaagcga tttcgcggcg
      541 tgacatcgag aagatgaaga acatcgagac cagcgaagtc gagcgcacgc tggcggtgga
      601 acaggcgcag gtggccaagc agatcgcgct gatcgcgaag gtgcgcgagc aacagacggc
      661 agagatcatg aagaagcaaa cggtggaagt ggccgagcgt aacaaggaag tggccgtcgc
      721 cgagaaggag cgcgagcgtg cgtcagcgca ggccgaagtg ttgaccgccg aagccgagcg
      781 cgaggcggct aagcagaagg tgctgacggt aacggtcagc agcgaagcgg accgcgaggc
      841 gcagaagaag ttgatcgcgg cccagcagct gatcaacgag aacaagatcc gcgaagagac
      901 cgccgccgac gtgtcggcgt acatggcggt gaagaaggca gaagccgagc gccaggcggc
      961 cgaggccatt taccaggcga agctgcgttt ggccgaaggt gattcgatcg cggcatcgaa
     1021 acgagccgac ggtgagaagg cgatcaagat ggtggacatc aacgtcgagc gcgaacgggt
     1081 gaacgtggaa caagcgcgcg tggaagtcga acgccaaagc ctggcgaaca agagcgagtt
     1141 cgaagaggcg gcgttgaaat tcgagctgga aaagttgcgg atcgacgccg agcgcgaggt
     1201 gcgcatccag gcggcccagg cgatgggaaa tatgttgagc aaggcgaaca tgcagatctt
     1261 cgccgacccc gacacgatgg cccgcatgca gggccagttc atgcgggccg ccagcctggg
     1321 cacggccgcg gatggattgc tcaaaagcct cccgccgcaa agccaggacg tgctggcgaa
     1381 gctggcgagc gcggtggcgt cgcagctgtc gccgaaggcg gccgccgcgg tggcggaaca
     1441 aaccggcaat ggaaacggcg cgacgccgat cggtgtgccg gtggcgacgg atgtcgcgcc
     1501 ggcggcggtt gattccaagc cgggcaagaa ggatcaccgg atgtcgtaat gtggttgatc
     1561 gccgcttgcg gcttagcatc ttttgaacgt caaacaaatg ctaagccgca agcggttaag
     1621 gatgacgacg atgacctgga aagatgacct caactacggc ctcgccgcgt tcgggattca
     1681 gatccccacc gacgtgccgc tcgaccaatg ggcgacgaag ctcaccggcc agcccgcgcg
     1741 caacaccgcc gccgtcgtcg cggccagcgc ggtcctgttc tacatcgccg agcgcgatta
     1801 caactcgaag gtgaacgaca tctgggacgc ctgcgtgtat tgctcgacgt gcctgagcgt
     1861 cggctacggc gacatcttcg ccaaaacccc cgcgggcaaa atcatcggca ccacactgat
     1921 gacgctcggc ccggcgttat ccggcgcggc gctcgacggc ccggcgccgc cgcagctgaa
     1981 tgatgatgtg caggcggaag tgctggcgac gctgaaggcg attctcgatc gcctgccgga
     2041 aaagtagccc ctgaccacat cattcaggct tttcatcgcc aacgagagtt tcagcttgcc
     2101 aacccgccgc ctcagcggat aattgcggcc acgcccaacg gagggcaagg ttcgcgcgcg
     2161 aggcattgca ttcagggtcc accgggggat ccatcatcgg cttaggccag cgaggacagc
     2221 cgcgcaatgc tgccgctggc ttagggtaac catttcggtt cttgcctcgc gaacacattg
     2281 taaaaatgga acgggtttca cctgccgcgc gcgcacgcgg gcagagggga gtcacagagc
     2341 atgcgccgaa catttccgtc gccgaagtgc cgtgctaatc agttgaccct tcgaggcgtg
     2401 cgagccatcc gcctcgcggc cctggcggcc gccgtatcgt tcgccggacc gcttgcgcct
     2461 gcgtcgcacg ccgatccgat caatcagatc gccacctgga ccggcggcgc gggcgacggc
     2521 aagtggtcca gcctcgccaa ttggtccccg cagaccgtgc cggcgaatga tgcgaacgat
     2581 ctgtacaacg tgatcgtcgg gaacaacgtg aacctgacgt ttgatttgcc gacggcggcg
     2641 acgatcaacg ggatcagctt cggcccgggc ggatcactgg cagtaggcag cggcgatcag
     2701 ttgacgatca cgaacgtcgc gtcgctcaac agcagcgttt caagcaacgg tgcgggcagc
     2761 atcttcacgg ccacgggcgt cggtgtcagc ctcggtccca acgcccacct ggcggccact
     2821 gcaggcggcg tcatcaacgt cgcgccgtca acgttcgttt acacgactgg cggcattcaa
     2881 gtgccggtct gcacggccga cggtgccggc tccgttgtca atgaatcgac gatgacgtcg
     2941 atgtccgcga acaacagtct aaccagttcg gacatccagc cggtctacac cgcgaccaac
     3001 ggtggcgcaa tcaacttgag cgggctactc tcaatcgacg gcggcatcgg cccgcacttc
     3061 atgaatatct tccgcgatgt cgcagtctcc gaaacgtatt tcgtcatcgg cgccaacagc
     3121 gacattaatc tcgccaacct caaacagatc accggatttg cgcagttcac cgtccagcgc
     3181 agcagttact ctctgccatc gcttcaaacc gcgtcagcca cgaccttcaa cctgagcgcc
     3241 ggcacggcaa tgaacctgcc ggcgctggtc acattggccg gcggaacatc cgcgctgaac
     3301 gtccccaata ccgcttcgtt caatgcgccg gttgcgacca cgctgaacga cacgcaattc
     3361 aacgtcagtg ccggcggcac cgtcaatgcc cacaatgtga cgacgctgga caattcaacg
     3421 ctggcgatcg ccgtgggtgg caccgtcgat atccacagcc tgcaggaggc aaccaacacg
     3481 ttcatcgcga tggcacccgg cgcgaacctg gattacacag cgccgatccg gttcgacaac
     3541 tctcggctga gcattaccgg cggcgcatcc ttcaacgtcg ccgcgaccag ctacacctat
     3601 acccgcaatg ccagcgagac ggcttggagt gccgacgggg caggctcggt tatcagtgca
     3661 cccagcatga cctccatggt ttcgacccaa agcttcatcg gcgacgtgcg gccggtatat
     3721 agcgcgacga acggcggggt gatcaacctg tcgggactgc agacgttcaa tggcggtgcc
     3781 ggcccgctca accaggtggc cgttggtagc agcttttcgt tcagcacgtt cttcatcgac
     3841 tcgagcagca atatcaaact cgacagcctg aaaacgcttt ccggctggaa cagtttcacg
     3901 atccagcgca gcagctacag ctttccgtcg ctgcagacca tgtcggcgac gacgttcacg
     3961 ctgaacgttg gtacgaccgt gaatgccccg gcgctaacaa ctgtcgcggg cggatcgtcg
     4021 tccatcccca tcccgagctt tgccagtttc aacgcgtctt ctctgaaaac actgaacgat
     4081 accacgttgt ccctcagcgt cggcggcgca ttcaccgcgc cgcagctttc gacgatccaa
     4141 aacaccaaca tcacgtttcc aagcggcgcc gacttcactt acgggacgat taagaacttc
     4201 gacgattcga ccgtttcgat cagcggcggc gcagtcttca atctcgccgt cccgtcatac
     4261 acattcgacc gcgattccac cgtgaccgcc tggcatacgg acggccccgg cacgaggatc
     4321 aattcgccga gcacgtcgtc gatcgtcgac acggtcggct tcccagcgta ccgggccatc
     4381 aatagcagca tgattgacct gtcggggttg cggttcgctc tcggtgccaa cacgttccag
     4441 gcgaactctg gcgggagcat tttgtttggt tcgctaaaga gctactccaa cttcaccgcc
     4501 gacggctcgg catcttcact caacttcaag tcgctcaatc tcacggcggg tggaacgctc
     4561 gtcgctacca acttggcaaa catcgtgctt cagggctcgc tcgcgtacac gacgacctct
     4621 ccatcatcga tcaacctgga tcaaggcgtg cttgatttcg ccgccgacag tcggc
//
LOCUS       DATIMH010000663         3985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2758082, whole genome shotgun sequence.
ACCESSION   DATIMH010000663 DATIMH010000000
VERSION     DATIMH010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2758082"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VLI90_11510"
     CDS             complement(<1..618)
                     /locus_tag="VLI90_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14880.1"
                     /translation="MTLTTDYKPKSRRPIADIFRGTARGAVALCVRLNIHPDAVSYGS
                     IAASAVAAICFWQSARWPWLLIVGPLLCYLRLWLNMLDGMVALASGKASRRGEILNDL
                     PDRISDVLIFAGVAHGGFCSPLIAYWAAILALFTAYVGTLGQAVGARREFGGVMSKPW
                     RMVALHVGAWITLGMLWWGDGTIRHGAWTVLDWTLLAILAGCVQTI"
     gene            complement(744..1508)
                     /locus_tag="VLI90_11515"
     CDS             complement(744..1508)
                     /locus_tag="VLI90_11515"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HSV14881.1"
                     /translation="MNNDWNDPRLRPIVDRQPYPLLFATISGAHLYGFPSPDSDFDLR
                     GAHILPPREMLGLSTGPETIESAGDEDGLEMDLVTHDVKKFFLLMLKKNGYVLEQLLS
                     PLVVHSTPAHQELKAIAPGCMTRHHAHHYLGFAETQWKLFEKESPRRVKPLLYVYRVL
                     LTGIHLMRTGEVKANLLKLNEIFRLPYVPELVAQKLAGPERSTLNEIDFTFHRAEYDR
                     LRAELESAYAASALPEAPSAQPALHDLLLRLRAGKA"
     gene            complement(1505..2434)
                     /locus_tag="VLI90_11520"
     CDS             complement(1505..2434)
                     /locus_tag="VLI90_11520"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HSV14882.1"
                     /translation="MPVTPLILSAGTQVVALVEIRGTNGKAVHPRGAVGVIIQSPADH
                     LHSYRVRFPDDFEASLKRQEISVLAQYQSGEIGNDPLAEHDLYQHVIYRCVIGSRAYG
                     LDTDASDIDRRGIYLPPAERHWSLFGVPEQLEKPSNEEVYWEIQKFLTLALKANPNVL
                     ECLYTPIVEHATPLAQELLAMRSAFLSKLVYQTYNGYVMSQFRKLQADLRNKRQVKPK
                     HVMHLIRLLLAGITTLREGVVPVHVGEQRDRLLEIRNGVAKFEEIDAWRLDLHRQFDA
                     AFEATKLPERPDYHAANALLIKARKHALTANAT"
     gene            complement(2492..3262)
                     /gene="lptB"
                     /locus_tag="VLI90_11525"
     CDS             complement(2492..3262)
                     /gene="lptB"
                     /locus_tag="VLI90_11525"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HSV14883.1"
                     /translation="MNLLETTKLVKTYGGRTVVNEVSFVLGQHEIVGLLGRNGAGKTT
                     TFRMILGMVTPDSGAVAFDATDITHLPMYKRARLGIGYLPQERSDFRRLTVWQNLMAI
                     LETLSISRGERNKRAAELLDQYGLTKTKNQRAETLSGGERRKLEIARALVTQPKLILL
                     DEPFSGVDPVAVEDLQREIVRLRNEFGKSVLITDHNVAQTLKVCDRALIIIDGVVKKE
                     GTPREIINDPEVRAAYLGHTFRGDEFDDREEALAFTSK"
     gene            3836..>3985
                     /locus_tag="VLI90_11530"
     CDS             3836..>3985
                     /locus_tag="VLI90_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HSV14884.1"
                     /translation="MATGKVKWFNDQKGFGFISSDNGAKDIFVHHSVIEGEGFRTLQD
                     GESVEY"
BASE COUNT          720 a         1167 c         1322 g          776 t
ORIGIN      
        1 gatcgtctgc acgcagccgg cgaggatcgc cagcaacgtc cagtcgagga ccgtccaggc
       61 gccgtggcga atcgtgccgt caccccacca caacatcccc agcgtgatcc atgcgcccac
      121 gtgcagcgcg accatgcgcc acggcttgga catcaccccg ccgaactcgc gacgcgcgcc
      181 gaccgcctgg ccgagcgtgc cgacgtaggc ggtgaacagc gcaaggatcg ccgcccagta
      241 ggcgatcaag ggcgagcaga agccgccgtg ggccacgccg gcgaagatga gaacgtcgga
      301 gatgcgatcg ggcaagtcgt tgaggatctc cccgcgccgg ctggccttgc ccgacgcgag
      361 ggcgaccatg ccgtcgagca tgttgagcca cagccgcagg tagcacagca gcgggccgac
      421 gatgagcagc cacggccagc gggcggattg ccagaagcag atggccgcga cggcggaggc
      481 ggcgatcgat ccgtacgaca ccgcatcggg atggatgttg agccgcacgc acagcgccac
      541 cgctccgcgc gcggtgccgc gaaagatgtc ggcgatcggc cggcgggact tgggtttgta
      601 atcggttgtc aaagtcatga aatttttcgg ctgggcgatc gagagccgtg atcgtacacg
      661 agatctcgcg gtaggttgct ctggtcccct cgcccggaga cctgccgtca gcagtaccgg
      721 gcgaggggac cagagcggac acctcatgct ttgcctgcgc gcaaacgcag cagcaaatcg
      781 tgcagtgccg gctgcgctga cggcgcttcg ggcagcgcgc tggctgcgta tgcggattcc
      841 aactcggcgc gaaggcgatc gtactcggcg cgatgaaacg tgaagtcgat ttcgttcagc
      901 gtcgatcgct cgggtccggc cagcttctgc gcgaccagct ccggcacata tggcaaccga
      961 aagatctcgt tcagcttcag caggttcgcc ttcacttcgc cggttcgcat caggtggatt
     1021 ccggtgagca ggacgcggta gacgtacagc agcggcttca cccgccgggg ggattctttt
     1081 tcgaacagct tccactgcgt ctcggcgaag ccgaggtaat gatgggcgtg atgccgcgtc
     1141 atacagccgg gggcgattgc cttcagctct tggtgcgcag gcgtgctgtg gacgaccagc
     1201 ggtgaaagga gttgctccag tacgtagcca ttctttttga gcatcagcag gaaaaacttt
     1261 ttcacatcgt gcgtgacgag gtccatctcc aggccatctt cgtcgcccgc cgattcgatc
     1321 gtctcgggcc cggtgctgag gccgagcatt tcgcgcggcg ggaggatgtg tgcgccgcgg
     1381 aggtcgaagt cggaatcggg ggagggaaag ccgtagagat gggcgccgct gatcgtcgcg
     1441 aagagcagcg ggtagggttg ccgatcgacg atcgggcgaa ggcgaggatc gttccaatcg
     1501 ttgttcatgt tgcgttagcg gtgagcgcgt gtttacgtgc cttgatcaac agcgcattgg
     1561 cggcgtgata gtcgggccgc tcgggcagtt tcgttgcttc gaacgcggca tcgaactgcc
     1621 gatgcaggtc gagccgccag gcgtcgatct cttcgaactt cgcgacgccg ttgcggatct
     1681 caagcaggcg atcgcgttgc tcgccgacat ggacgggcac gacgccctcg cgcagcgtgg
     1741 tgatgccggc cagcaacagg cggatcaggt gcatcacgtg cttcggcttc acttggcgct
     1801 tgttgcgcag gtccgcctgc agcttgcgga attggctcat cacgtagccg ttgtaggtct
     1861 ggtagaccag cttggacagg aacgcgctgc gcatcgcgag cagctcctgc gccagcggcg
     1921 tcgcgtgctc gacgatgggc gtgtacaggc actcgagcac gttcgggttc gccttcagcg
     1981 ccagcgtcag gaatttctgg atctcccaat agacctcttc gttggacggc ttttcgagct
     2041 gctcgggcac gccgaacagc gaccagtggc gctcggccgg cggcaggtag atgccacggc
     2101 gatcgatgtc gctggcgtcg gtgtcgaggc catacgcgcg cgagccgatc acacagcgat
     2161 aaatgacgtg ctgatataga tcgtgctcgg ccagcgggtc gttcccaatc tcaccggatt
     2221 gatactgagc gagaaccgag atctcctgcc gcttaagcga ggcttcgaag tcgtcgggaa
     2281 agcgcacgcg gtaactgtgc agatgatcgg ccggagactg gatgatcacg ccaacggcgc
     2341 cccgcgggtg cacggccttg ccgttggtgc cacggatttc aaccagcgcg acaacctggg
     2401 tgccagcgga gagaatgagc ggcgtcaccg gcatcaatgc ggggaagcta ccacaccggc
     2461 ccgacccgct taagcgggtc ggcctgtgcg cttactttga cgtgaacgcc agcgcttcct
     2521 cgcgatcgtc gaactcgtcg ccgcggaagg tgtgaccgag ataggccgcc cgcacttccg
     2581 ggtcgttgat gatctcccgc ggcgtgcctt cctttttcac gacgccgtcg atgatgatca
     2641 gcgcccgatc gcacaccttc agcgtctgcg cgacgttgtg gtcggtgatc aagacgctct
     2701 tgccgaactc gttgcgcagg cgaacgatct cgcgttgcag gtcttcgacg gcgacggggt
     2761 cgacgccgct gaacggttcg tcgagcagga tcagcttggg ctgcgtgacg agcgcgcggg
     2821 cgatctccag ctttcggcgt tcaccgccgc tgagcgtctc ggcccgctgg ttcttggtct
     2881 tggttaaccc gtattggtcg agcaactcgg cggcgcgctt gttgcgctcg ccgcgggaga
     2941 tgctgagcgt ttcgaggatg gccatcaggt tttgccagac ggtcaaccgg cggaagtcgc
     3001 tgcgttcctg cggcaggtaa ccgatgccca ggcgggcgcg cttgtacatc ggcaggtggg
     3061 tgatgtcggt ggcgtcgaag gcgacggccc cgctgtcggg cgtcaccatg ccgaggatca
     3121 ttcggaaggt ggtggtcttg ccggcgccgt tgcggccgag cagaccgacg atctcgtgct
     3181 gcccaagaac gaagctgacc tcattgacga cggtccgccc gccataagtc ttgaccagtt
     3241 ttgtggtttc cagaaggttc atagcgggat tgacctgagc cggcgatcag tctgccgacg
     3301 gctgctcgaa aatcaagcaa agcgcttgtg gataacgtgt ggacgaaccc gaagcgggag
     3361 tttaggcagt ttacggaccc gaaatgaatg aacggttgcc ggggagtccg tgaggtcatg
     3421 gaccgcctta gaatttgcca cctttaccgc ccgctaacat gcaaactgct gcagctatag
     3481 cggtttagcc ccggtcaggg atgacctgag gccgcgggta atgtggagaa cgcatcgtct
     3541 tttggaaccg gctcgaagcg agatcgagcc gaaattcagc gccggcggtt gacattcgcc
     3601 tgttgtggat aactccgtgc agcaggtcgc aattttcacc gttgacacgt attgcgaatt
     3661 tttttgtttc cgcggctcag catggtattg tctgatccac aaatccgttc ccctggcacg
     3721 gggacgcatt ccgacgggga gggcccggcg ggatcgaagg tgccgcatct ttttgccgga
     3781 cgaccctcgc atttcgtggg tcgccgcgcg tcacgtcttg aaaggagtgg cttttatggc
     3841 cactggtaag gtcaagtggt tcaatgacca gaaggggttc ggtttcatct cttccgacaa
     3901 cggagcgaag gacattttcg tccatcattc cgtgatcgaa ggggaaggtt tccgtaccct
     3961 tcaggacggt gaatccgtgg agtac
//
LOCUS       DATIMH010000664         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5647986, whole genome shotgun sequence.
ACCESSION   DATIMH010000664 DATIMH010000000
VERSION     DATIMH010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5647986"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VLI90_11535"
     CDS             <1..888
                     /locus_tag="VLI90_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV14885.1"
                     /translation="KRESSSESEGNGDASQWIVKEKPSIRFADVAGLDDVKEDIRLKM
                     IYPFEHPELAEKFGVRPGGGVLLYGPPGTGKTMLAKATAGEIDATFFRISPADVLSKW
                     VGEAEQNIKKLFDAASSEKRSIVFIDEIEALVPARRDEGSSVMQRVVPQILQGMEGFD
                     KKSGSPILFMGATNVPWQLDPAVLRPGRFDEKVYIPLPDLPARRKMLDIYLSKRPLDP
                     DVNLDDLAQKLDGFSGADIKYICDRSATIPFLRSVATGNEGDITPEILLDVINEAQRS
                     VTPEMLHRLNEWGRVASAN"
     gene            878..1210
                     /locus_tag="VLI90_11540"
     CDS             878..1210
                     /locus_tag="VLI90_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14886.1"
                     /translation="MPTDSVSPVDASSPAPFPARKTNFGAVALAFAAALVLPMIVGEI
                     AMRFRLPLPRNIEQNVSDFIFDYGFIWFLFTLLFIVIGLTRRGSSRRCAPVALGLLLI
                     SVLLLFVG"
     gene            1280..1705
                     /locus_tag="VLI90_11545"
     CDS             1280..1705
                     /locus_tag="VLI90_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14887.1"
                     /translation="MDNLDVMGFNRLSMLTISCLMLCIGTLGFWWRSSQFVDTVSLGS
                     VNVWTMDGKVALVSAPGPSRQSCDWMSRPRPPMATVDWPLFRFHPAGVDPVSGRATAA
                     GFVVPVWFFTVLLVLPPLVNAQIQHRRNLRRELMSETTG"
     gene            1891..>2615
                     /locus_tag="VLI90_11550"
     CDS             1891..>2615
                     /locus_tag="VLI90_11550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HSV14888.1"
                     /translation="MSDLREHLQNHFGLDDFRPRQREVIEDVLGGKDVLCVMPTGAGK
                     SLCYQLPAIVGGGLTVVVSPLISLMEDQVQQLSDEGLPALLLNSSLNAAEQREVIAKL
                     HQDFNGLLYVAPERFFAASFRSTLDQLRPKLFAVDEAHCVSQWGHDFRPEYSRLGDVR
                     ERLGNPPTIALTATATDDVREDIVLNLRLSEPSIVITGFDRPNLAYESRRVAKVAEKT
                     NELLGMFRQEPGSAIVYCATRKAV"
BASE COUNT          461 a          804 c          815 g          535 t
ORIGIN      
        1 aagcgcgaat cctccagcga atccgaaggc aacggcgatg cgtcccaatg gatcgtcaag
       61 gagaagccca gcatccgctt cgccgatgtc gccgggctcg atgacgtcaa ggaagacatc
      121 cgcctgaaga tgatttaccc attcgagcac cccgagctgg ccgaaaaatt cggcgtccgc
      181 cccggcggtg gtgtgctgct ctacggcccg cccggcacgg gcaagacgat gctcgccaag
      241 gccaccgccg gcgagatcga cgcaacgttc ttccgcatct cgcccgccga cgtgctcagc
      301 aaatgggtcg gcgaagccga gcagaacatc aagaaactct tcgatgctgc cagctctgaa
      361 aagcgcagca tcgtcttcat cgatgagatc gaggcgctcg tgcccgcccg gcgcgacgag
      421 ggcagcagcg tgatgcagcg cgtcgtcccg cagatcctgc aaggcatgga aggtttcgac
      481 aaaaagagcg gctcgcccat cctgttcatg ggcgcgacca acgtcccctg gcaattggac
      541 ccggcggtcc ttcgccccgg ccggttcgat gagaaagtct acatcccgct gccggacctg
      601 ccggcccggc ggaagatgct cgacatctac ctcagcaagc gcccgctcga tcccgatgtg
      661 aacctcgacg atctggcgca gaagctggat ggttttagcg gcgccgacat caaatacatc
      721 tgcgaccgct cggcgacaat cccgttcctg cgctccgtcg ccaccggcaa cgaaggcgat
      781 atcacgcccg agattttgct ggacgtgatc aacgaagcgc agcgatcggt gacaccggag
      841 atgcttcatc gattgaacga atggggacgc gtcgccagtg ccaactgatt ctgtttcacc
      901 ggtggacgct tcctcgcctg ctcccttccc ggcacgcaag acgaactttg gcgcagtggc
      961 gctggccttc gcagcagcgc ttgtcctgcc gatgatcgtg ggcgagatcg ccatgcgctt
     1021 ccgccttcct ttgccgcgaa atatcgagca gaacgtcagc gacttcattt tcgactatgg
     1081 tttcatctgg tttctcttca cgttgttgtt cattgttatc gggctgaccc ggcgtggctc
     1141 cagtcggcga tgcgctccgg ttgcattggg tctgcttctc atatcagtgc tccttttgtt
     1201 cgttggctga tccgcataac cgctacatgt ccccaatcgc acgaattcgc agcttcgact
     1261 ggcctcgctc tagtttgaat tggataacct ggacgttatg ggcttcaatc gactgagcat
     1321 gttgacgatc tcgtgcctga tgctctgcat cggcacgctg ggcttctggt ggcgcagcag
     1381 ccagtttgtt gacaccgttt cactcggttc cgtgaacgtg tggacgatgg acggcaaggt
     1441 ggcgctggtg tcggccccgg ggccgtcgcg gcaatcctgc gattggatga gccggccgcg
     1501 gcctccgatg gcgaccgtcg attggccgct gttcaggttt catccggccg gggtggatcc
     1561 ggtgagtggt cgcgcgacgg cggcggggtt tgttgtgccg gtttggtttt tcacggtgtt
     1621 gctggtgctg ccgccgctgg tcaatgcgca gattcaacat cggcggaacc tgcggcggga
     1681 gttgatgtcg gagacgacgg ggtgaatagc gtgcggggat ggttgaagat tcgctcggcg
     1741 ctgaacgacc ggcgccggac gctcggccct tcgggccgcc gctaaaaggg atgtccgaat
     1801 tgtgagattt gatttagacg acgtgcgtga atgaccgctt gcgcgcgtgc cgtgcttctg
     1861 cggcgcggtt tgtctataat tcccatggcg atgtctgacc tgcgggagca cctccaaaat
     1921 cattttggcc tcgacgattt ccgcccgcgg cagcgggagg tgatcgagga cgtgctgggc
     1981 ggcaaagacg tgctgtgcgt catgcccacc ggggcgggca agagcctttg ttatcagctg
     2041 ccggccatcg tcggcggcgg gctgaccgtc gtcgtctcgc cgctcatctc gctgatggag
     2101 gaccaggttc agcaattgag tgacgagggg ctgccggccc tgctgttgaa cagttcgctg
     2161 aacgcggccg agcagcgcga ggtgatcgcc aagctgcatc aggatttcaa cgggctgctg
     2221 tacgtcgccc ccgaacgctt cttcgccgcc agtttccgat ccacgctcga ccaactgcgt
     2281 ccgaaactgt ttgccgttga cgaagcgcac tgcgtcagcc aatggggtca cgatttccgc
     2341 cccgagtatt cgcgtctcgg cgacgtgcgc gagcggctgg gcaacccgcc gacgatcgcg
     2401 ctgaccgcca ccgccacgga tgacgttcgg gaagacatcg tcctgaacct tcgcctcagc
     2461 gagcccagca tcgtcatcac cgggttcgat cggcccaacc ttgcgtacga atcgcgccgc
     2521 gtcgcgaagg tggcggagaa gacgaacgaa ctgctcggca tgtttcgcca ggaacccggc
     2581 agcgcgatcg tctactgcgc gacgcgcaag gccgt
//
LOCUS       DATIMH010000665         6964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_449739, whole genome shotgun sequence.
ACCESSION   DATIMH010000665 DATIMH010000000
VERSION     DATIMH010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449739"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="VLI90_11555"
     CDS             <1..1756
                     /locus_tag="VLI90_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV14889.1"
                     /translation="VVNQGGLLQTSAADVGGPGGGDANIFGPITLNGATFTAGNGYNA
                     DYQAVILLKDVTVGGVAASAINTNASNTTANSIMLGDAASGTDAITFNVADATNNASA
                     DLTVSAKLTNSANSTSNPAAIGALTKAGAGTMVLSGANTYSGPTTINAGTLQMNSTNA
                     TSASVTVNSGGTLALNAGDALGVATGKNVLTINSGTVSNNMSTGRVTIWNDLTMTGGT
                     LTGTGSGDGNGAYTLNTQVIATSDAGGNAATISGGPIGLQNRNVSGGSITFNVARGPA
                     TPASDLIVSANLIPVSFASGIGITKSGNGIMMLSGANTFTAATSVNGGTLTIAAAGSM
                     ASTSYTVAGGATLNVSGVIPAGSVIADSGTVSFAGNGSGFIATRNVAALNITGGGVAK
                     VLTSTAGAATPLVLKPATLSIDAASNLDLTNNELVAPGSADSALMLIQSGQVFSSALA
                     DANHALGYISVPSSSNMEVRYALKGDANLDGSVDVGDLGALATSYGASGGQSWINGDS
                     NQDGNIDVGDLGALATNYGSSLASSPAEAAVATAVVADGASTVPEPAMVGMLVLAALC
                     HPERRRNRRSRRILSRSR"
     gene            1959..2309
                     /locus_tag="VLI90_11560"
     CDS             1959..2309
                     /locus_tag="VLI90_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14890.1"
                     /translation="MKSKRNSALLTAAAAAIALLAKRSAHAGLTFNVDPNSPWPAGWY
                     NAAVADMQTVVNEYNAYGNFTQDNSGNIYVYYNAGIPTAQSDDNGSRYRPGRSAASAG
                     YPAVPAGGSGRWPR"
     gene            complement(2405..3283)
                     /locus_tag="VLI90_11565"
     CDS             complement(2405..3283)
                     /locus_tag="VLI90_11565"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSV14891.1"
                     /translation="MKVFVAVAENLSFTRAAESLYLTQSAVSHQIAKLEQQLGVALLL
                     RQGRAVSLTAVGQSLLTHARRVFAALEDAAAAVRQAARPDAGRLRIGATSTACQYLIP
                     EALREFRECFPGYSLSILPGDSPVVAERLTEGSIDLAVMIRHERRSRMVYHHLFTDEL
                     GMLVSPLHPWAKSGKVDRQQLGEQRLVHYSRNSATFRLVDRYFARMRAPLHDPIELGS
                     MEAIKELVKLGLGVSVVARWIARSEIAERSLVWLPLPGGRLKRSWCIATPSGKKLSVA
                     EQTFIGLCQDAAGALR"
     gene            3479..4627
                     /locus_tag="VLI90_11570"
     CDS             3479..4627
                     /locus_tag="VLI90_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HSV14892.1"
                     /translation="MPAAGSRQKLQTLARPERGAATTLQIGFVPLIDAAPLIAAQVLG
                     YFADEGLTVTLDRQIGWGNIRDKLTFGQLQAAHALLGMPLYSALGREWFSEPLLAVMN
                     LGCGADAITFSQRLIGAGITGANSLAAWRRRSRGDMPQAIAHVFDCSVHHYLLRMWLA
                     AGEVDVERDARLCVLPPPQVARHMAKGYLDGFCCGEPWNSLAARERTGRIVAVTSDII
                     PSHPDKVLAVNQRWANAHPELVKPLVRAILRGMQFCSDAANLSRVVEVLAAPQHIGVD
                     ATVIDESLRSGSRWRGFDTANGFPSVTHHAWYAAEMTRWRHLSPDGDIPELARRCVSA
                     RAFREAAASLGIDCPDTDEPAMRLRNGFFTIESSSNAAATKCPLAAVV"
     gene            4648..6000
                     /locus_tag="VLI90_11575"
     CDS             4648..6000
                     /locus_tag="VLI90_11575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV14893.1"
                     /translation="MDIRHKAPRINLLDFTSPQMRAFHMSWFAFLMCFFAWFGIAPLM
                     NQVRTELHLTPAQVGNTIIASVAITVFARLLAGWLCDRIGPRLTYTGLLILGSLPVMG
                     IALVHGYGSFMLMRLAIGVIGASFVITQYHTSIMFAPNIVGTANATSAGWGNMGGGVT
                     QFVMPALFLWFISHGMSRSGSWRASMVAAGVVCLLTGVAYFLFTQDAPDGNFKELRAR
                     GQLPQKKQGFAGFTAALRDPRVWVLFVLYGCCFGIELTIDNIAHLYFTDYFHMGLTTA
                     GWTAAIFGLMNLFGRALGGIFGDRIGITRGLRGRAMWLFAVILAEGLFLILFSRMTLI
                     APMLLALLAFSLCVQMGCGATFSVVPFINKKSLGSVSGVVGAGGNAGAVAAGFLFKGS
                     IAWPTGLLILGIAVTACSFLALAITFSTADESAARQEMEARLAEQRERESEVAVPAMA
                     "
     gene            6022..>6964
                     /locus_tag="VLI90_11580"
     CDS             6022..>6964
                     /locus_tag="VLI90_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14894.1"
                     /translation="MSTSSNDTDLGGDEQQFYREGIAAAVELVKAAPTWAAALGLPQQ
                     SKPRDAGGDITGPDALNRAAQDRFIAAGTKLTAEEQAKRKLNGLDTWDLVLDHARDGR
                     FPKGTDVFLMKFQGMFYVAPAQDSFMCRLRIPGGVLSSHQLRGVAQLAEAFAGGYADV
                     TTRANLQLREIGPKHTVDVLMGLHDLGIVNRGAGADNIRNVTASPTAGIDLQELIDTR
                     PLARQMHHYILNHREMYGLPRKFNIAFDGGGRVSALEDTNDIGFTAVGVGEGCEIPNG
                     VYFRLTLGGITGHKDFARDTGVLLKPEECVPVAAAIVK"
BASE COUNT         1231 a         2182 c         2314 g         1237 t
ORIGIN      
        1 cgtcgtcaac caaggtggtt tgctacagac gagcgcggcc gatgtgggcg gccccggcgg
       61 cggggacgcg aacatctttg gtccgattac gctcaacggc gcgacgttca ccgcgggcaa
      121 cggctacaac gccgattacc aggcggtcat cctgctgaag gacgtgaccg tcggcggcgt
      181 ggccgcgtcg gcgatcaaca ccaacgcgtc gaacaccact gccaatagca tcatgctcgg
      241 cgacgccgcc agcgggaccg atgcgatcac gttcaatgtc gccgatgcga caaacaacgc
      301 cagcgccgac ctgaccgtct cggcgaagct gaccaactcc gccaactcca ccagcaaccc
      361 cgcggccatc ggcgcgctga ctaaggccgg cgccggcacg atggtcctct ccggcgcgaa
      421 cacctacagt ggcccgacca cgatcaatgc cggcacgctg cagatgaaca gcaccaacgc
      481 cacgtcggcg tcggtcaccg tcaacagcgg cggcacgctg gcgctgaacg ccggcgatgc
      541 gctgggggtc gcgaccggca agaacgtgtt gacgatcaac agcggcaccg tctcgaacaa
      601 catgagcacg gggcgcgtga cgatctggaa cgacctgacg atgaccggcg ggaccttgac
      661 cggcaccggc agtggcgatg gcaacggggc atacacgctg aacacgcagg tgatcgcgac
      721 atcggatgcc ggcgggaacg ccgcgacgat cagtggcggg ccgattggtt tgcagaaccg
      781 caacgtgtcc ggcggctcga tcacgttcaa cgtcgcgcgc ggcccggcga cgccggcgtc
      841 ggacttgatc gtttcggcga acctcatccc cgtcagcttc gccagcggga tcggtatcac
      901 caagagcggc aacggcatca tgatgctgag cggggcgaac accttcaccg ccgccacgag
      961 cgtgaacgga gggacattga cgatcgcggc cgctggttcg atggcgagca cgagctacac
     1021 ggtcgccggc ggggcgacgt tgaacgtgag cggcgtgatt cccgccgggt cggtgatcgc
     1081 ggacagcggc acggtcagct tcgccggcaa cggcagcggg ttcatcgcga cgcggaacgt
     1141 cgctgcgctg aacatcaccg gcggcggcgt ggctaaggtg ctcacatcca cggctggcgc
     1201 ggcgacgccg ctcgtgctaa agccggcgac gctgagtatc gacgcggcga gcaatctgga
     1261 tttgacaaat aacgagttgg ttgcgccggg cagcgcggac tctgcgctga tgctgatcca
     1321 aagcgggcag gtgttcagct ccgcgctggc ggatgcgaat cacgcgctgg gctacatcag
     1381 cgtgccatcg tcgagcaaca tggaggtccg ctatgcgctg aagggtgacg cgaaccttga
     1441 cggcagcgta gacgttggcg atctcggtgc cctcgcgacc tcgtacggtg ccagcggcgg
     1501 gcaatcgtgg atcaacggcg acagtaatca agatggcaac atcgatgtcg gcgacctggg
     1561 cgcgctggcg acgaactacg gctcatctct cgcgagcagc cccgcggaag ctgctgtcgc
     1621 gacggcggtg gttgccgatg gggcgtcaac cgtgccggag ccggcgatgg tcggcatgct
     1681 cgtgctggcc gcattatgtc atcctgagcg caggcgtaat cgccggagtc gaaggatctt
     1741 gtcgaggagt cgatgaagcg ctgcttcgcg aagaacgtca cagccgcttg cggctttgcg
     1801 tctgttctac attgcgaaag atgctaagcc gcaagcggct gttgttgcga agctggcgct
     1861 tcacttactt ctcggctaga tccttcgact gcgcatgagt acatgctccg ctcaggatga
     1921 caggcgatct cggtctcagc cgcgggtgga acgagcaaat gaagtccaaa cggaacagcg
     1981 ctctgttgac tgccgccgcc gcggcgatcg cgcttttggc gaagcgctcg gcgcacgctg
     2041 gcttgacctt caacgttgac cccaacagtc cctggccggc gggttggtac aacgccgccg
     2101 tcgccgacat gcagacggtg gtgaacgagt acaacgccta cggcaatttc acgcaggaca
     2161 attcgggcaa catctacgtc tactacaacg ccggcattcc gaccgcgcaa tccgatgaca
     2221 acggcagcag ataccgcccc ggtcggtccg ctgcatcggc cggttatccg gcagttccgg
     2281 ccgggggtag cgggcgctgg ccgcgctgac cggcgagcgg cacgaaattc agtggacatg
     2341 acaatcgcct ccatcggaac gcgttctgac cgacgaatga tgcttgatca tgccagcttt
     2401 cacgctagcg cagcgcccct gccgcgtcct ggcataggcc gatgaaggtt tgttcggcaa
     2461 cggaaagctt tttgccgctg ggggtggcga tacaccagct gcgtttgagg cggccgccgg
     2521 gcagcggcag ccagacgagg gatcgctcgg cgatctcgct tcgcgcgatc catcgcgcaa
     2581 ccacgctcac acccaggccg agcttcacca gttctttgat cgcctccatc gagcccagtt
     2641 cgatcgggtc gtgaagcggc gcgcgcatgc gggcgaaata tcgatcgacc aaccggaacg
     2701 tcgcgctgtt gcggctgtag tgcaccagcc gttgctcgcc gagctgttgg cggtccacct
     2761 tgccggactt ggcccacgga tgcagcgggc tgacgagcat gcccagttcg tccgtgaaca
     2821 ggtggtggta caccatgcgc gaccggcgtt cgtggcggat catgacagcc aagtcgatcg
     2881 agccttcggt caatcgctcg gcgacgaccg ggctgtcgcc gggcaggatc gacagcgagt
     2941 acccggggaa gcattcgcgg aattctcgca gcgcttcggg gatgaggtat tggcaggcgg
     3001 tgctggtcgc gccgatgcgc aggcgaccgg cgtcgggtcg cgcggcctgt cgaaccgcgg
     3061 cggccgcgtc ttccagcgcg gcaaacaccc gccgggcatg ggtcagcagc gattgtccga
     3121 ccgccgtcag cgaaaccgcc cgtccctgcc gcagcagcag tgccacgcct aactgctgct
     3181 caagcttggc gatctgatgg ctgaccgccg attgcgtcaa atacagcgac tcggccgccc
     3241 gggtgaagct taagttttcg gcaaccgcga cgaaaacctt caattgatgc acatcttcca
     3301 cgcatgctcc atgaattcat tcgatttgaa ggatcgtaac aatgaattgg attcatgcaa
     3361 atgatcccgt tacatttccc ccatcggtgc ggcggagcag gcaacgcgag gaacgacatc
     3421 agggtttggc agtgcaccgg ttgcgagcga catttgagga tgggttggaa aggtcttcgt
     3481 gcccgccgcc ggttcccggc aaaaacttca gacgctggct cgccccgagc gcggagcggc
     3541 aacgacgctg cagatcgggt tcgtcccgct gatcgacgcg gcgccgctga tcgcggcgca
     3601 ggtgctcggt tacttcgccg acgaagggtt gaccgtcacg ctcgatcgcc agatcggctg
     3661 ggggaacatc cgtgacaagc tcacgttcgg ccagttgcag gcggcccacg cgctgctcgg
     3721 catgccgctg tacagcgcgc tgggccgcga atggttcagc gaaccgctgc tggcggtgat
     3781 gaacctgggc tgcggcgcgg acgcgatcac cttcagccag cgattgatcg gcgccggcat
     3841 caccggcgcg aattcattgg ccgcctggcg gcgccggtcg cgcggcgaca tgccgcaggc
     3901 catcgcgcac gtgttcgact gttcggttca ccattacctg ctgcggatgt ggctcgccgc
     3961 cggtgaagtg gatgtggagc gcgacgcgcg gctgtgcgtg ctgccgccgc cgcaggtggc
     4021 acggcacatg gccaagggtt acctcgacgg attctgctgt ggcgagccat ggaattcact
     4081 ggccgcgcgg gaacgcaccg ggcggatcgt cgccgtcacg tcggacatca tcccgtcgca
     4141 tccggacaag gtgctggcgg tgaatcagcg gtgggcgaac gcgcatccgg aactcgtgaa
     4201 accgttggtc cgcgccatcc tgcgcggcat gcagttctgc agcgacgctg caaacctctc
     4261 gcgcgtcgtc gaggtgctgg cggccccgca gcacatcggc gttgacgcga ccgtcatcga
     4321 cgaaagcctg cgaagcggct cgcgctggcg cgggttcgat accgccaatg gattccccag
     4381 cgtcacgcat cacgcctggt acgccgccga gatgacgcgc tggcggcacc tttcccccga
     4441 tggagacatt ccggagttgg cgcggcgctg cgtctcggct cgcgcgtttc gcgaagcggc
     4501 ggcgtcgctg ggcattgatt gccccgacac cgacgagccg gcgatgcggc tgcgaaatgg
     4561 tttcttcacg atcgaatcgt cgagcaacgc ggcagccacc aaatgcccgc tggcggcggt
     4621 ggtttgagcg cagggatttt ttcaaccatg gatattcgcc acaaagcccc gcgcatcaac
     4681 ctgctcgact tcacgtcgcc gcagatgcgg gcgtttcaca tgagctggtt tgccttcctc
     4741 atgtgcttct tcgcctggtt cggcatcgcc ccgctgatga accaggtgcg gaccgagctt
     4801 cacctgacac cggcgcaggt cggcaacacg atcatcgcat ccgttgcgat tactgtgttc
     4861 gcaagattac tggccggctg gttgtgcgac cgcattggac cgcgtttgac ttacacgggg
     4921 ctgctgattc tcggttcgct gccggtgatg ggcatcgcgc tggtgcacgg ttacggctcg
     4981 ttcatgttga tgcggctggc gatcggtgtg atcggggcgt cgttcgtcat tacgcaatac
     5041 cacacgtcga tcatgttcgc gccgaacatc gtcggcaccg ccaacgccac cagcgcgggc
     5101 tggggcaaca tgggcggcgg tgtgacgcag ttcgtcatgc cggcgctgtt tctctggttc
     5161 atctcgcacg gcatgagccg cagcggcagc tggcgggctt cgatggtcgc cgccggtgtc
     5221 gtctgtttgc ttacgggcgt cgcgtacttt ctgttcacgc aggacgcgcc cgatgggaac
     5281 ttcaaagagc tgcgcgcccg cggccagctg ccgcagaaga agcaaggatt cgccggcttc
     5341 accgccgcgt tgcgcgaccc gcgggtgtgg gtgctgttcg tgctgtacgg ctgctgtttc
     5401 ggcatcgaac tgacgatcga caacatcgcg catctgtact tcaccgacta cttccacatg
     5461 ggcctgacca ccgcggggtg gacggcggcc atcttcgggt tgatgaacct gttcggacgc
     5521 gcgctcggcg ggatcttcgg agatcgcatc ggcatcaccc gcggtctgcg cggccgggcg
     5581 atgtggctct tcgcggtcat cctcgccgag ggattgttcc tgatcctgtt cagccgaatg
     5641 acactcattg cgccgatgct gctcgcgctg ctggcgttca gcttgtgcgt gcagatgggg
     5701 tgcggggcga cgttctcggt cgtgccgttc atcaacaaga aatcgctggg atctgtatcc
     5761 ggcgtcgtcg gcgctggcgg gaatgcgggc gcggtggccg ccgggtttct gttcaaaggc
     5821 agcatcgcgt ggccgacggg gctgctgatc ctgggtatcg cagtcaccgc atgttcgttc
     5881 ctggcgctgg cgatcacgtt ctcgaccgcc gacgaatcag ccgcgcgaca ggaaatggaa
     5941 gcgcgcctgg cagagcaacg cgagcgcgag tccgaggttg ccgttccggc gatggcgtaa
     6001 aagcagcgtg agatttgcaa catgagtacg agcagcaacg acaccgatct gggcggggat
     6061 gagcagcagt tctaccgcga aggcatcgcc gccgcggtgg agttggtgaa agcggcgccg
     6121 acgtgggcgg cagcgctcgg cttgccgcag cagtcgaagc cgcgcgacgc cggtggcgac
     6181 atcaccggtc ctgatgcgct caatcgggcg gcgcaggacc gcttcatcgc cgcgggaacg
     6241 aagttgacgg cggaggagca ggcgaagcgg aaactgaatg ggctggatac atgggatctg
     6301 gtgctcgatc acgcccgcga tggccggttt cccaagggca ccgacgtttt cctgatgaag
     6361 ttccagggga tgttttacgt cgcgccggca caggattcgt tcatgtgccg gctgcgcatt
     6421 cccggcggcg ttctcagctc gcaccaactg cgcggcgtcg cgcagctggc cgaggcgttc
     6481 gccggtggat acgccgatgt gacgacgcgg gcgaacttgc agctgcgcga gatcgggccg
     6541 aagcacacgg tcgatgtgct gatgggcctg cacgacctgg gcatcgtcaa tcgcggcgcg
     6601 ggcgccgaca acatccgcaa tgtcaccgcc agtccgaccg cgggaattga cctgcaggaa
     6661 ctgatcgaca cgcggccgct ggcccggcag atgcaccact acatcctcaa ccaccgcgag
     6721 atgtacggcc tgccgcggaa attcaacatc gccttcgacg gcggcgggcg cgtgagcgcg
     6781 ctggaagaca cgaacgacat cggcttcacc gccgttggcg ttggtgaggg ttgcgaaatt
     6841 ccaaacggcg tttacttccg cctcacgctc ggtgggatca ccggtcacaa ggatttcgcc
     6901 cgtgacaccg gcgtcctgct caagccggag gaatgcgttc ccgttgcggc cgcgatcgtc
     6961 aaag
//
LOCUS       DATIMH010000666         4025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3627306, whole genome shotgun sequence.
ACCESSION   DATIMH010000666 DATIMH010000000
VERSION     DATIMH010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3627306"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VLI90_11585"
     CDS             <1..301
                     /locus_tag="VLI90_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14895.1"
                     /translation="TPDDDAHRAGEREVKDELTFKGGTFASAAFAKRGFDPGPYDEEI
                     HPGSVGGFSAIQNNKSGDGNIKWNGFVAAGELSGTLVWTKKDGTVLNYTFKGSKE"
     gene            complement(504..1823)
                     /locus_tag="VLI90_11590"
     CDS             complement(504..1823)
                     /locus_tag="VLI90_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14896.1"
                     /translation="MERDRNASRKHDDGFDTSQWGQTILWLCILLLTAAVVASAANPP
                     AAAAHAATYTDGKLSVTLTRVGNNISGTVTLNGQTFPLKATADKNDPMKMFGSFTANG
                     KTYQIAMGVPAKGQLNFKTGTTLYHLSMANPGADNPLVAPATPQGTDASNPLGESAMS
                     NAGNDANSPLASATTPPSGVSSTPPAPPPVPVAPAYVRPTLRVQQGQYFRWSQPEGWR
                     SNETTNGVDVISPDGRAGATSCLLVNTPGTTNPQGFVQGFLPRCGAKNIQITAVRDLP
                     DQMYAGAPMKTQQMELTYDVNGTAYHGLMTCAVVTGFNNYTAFLQGAASVPEDWDKVK
                     TWLPAIAENVSITNPQQVAMQDKIILPKNRPLDDTSVMSAYQTRAASQDRMSQKWEET
                     ALGYERMKDPGTGQMYDMPFEAFDAARGGYVNPQRPTDLLQHAQAGE"
     gene            1955..2683
                     /locus_tag="VLI90_11595"
     CDS             1955..2683
                     /locus_tag="VLI90_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSV14897.1"
                     /translation="MTVTLNTLRQDVESCTRCPRLVTYRAAVAVAKRRAFADWDYWGK
                     PVAGFGDPAARLWIIGLAPAAHGANRTGRIFTGDRSGDFLFAALYRCGLANQQKSVSR
                     EDGLALSDCFISATARCAPPANKPLPEEVENCSGYLDQEWRLLGKKRVILALGGIAWN
                     ASLTLAARNGCQIARPRPAFGHGAELQLCPGLTLVGSYHVSQQNTFTGRLTPAMFDGV
                     IKRAMALCRSTRKPRMYVPGSGAT"
     gene            complement(2760..3200)
                     /locus_tag="VLI90_11600"
     CDS             complement(2760..3200)
                     /locus_tag="VLI90_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV14898.1"
                     /translation="MQVEPAQADETTPLPAPPPRLLVVDDAAVERLALAHYLRKNGYD
                     VIEAGDGNSAVLHLKNTQVDLLLLDLNMPQGDGFSVLNYIQSHRRSLPVVLLSGMPVD
                     EIQHKMHRLREQELPPLLLKPVDPEQLMQIVELQLSGGMTDLGE"
     gene            3272..3535
                     /gene="xseB"
                     /locus_tag="VLI90_11605"
     CDS             3272..3535
                     /gene="xseB"
                     /locus_tag="VLI90_11605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HSV14899.1"
                     /translation="MGKKNQTPPKNFEEALGELEQILADIEGGEIGLEESITKYERGT
                     FLIQHCRGVLSTAEKQIEVLSKGSDGELQSEPMSDEAAEGSAG"
     gene            complement(3536..>4025)
                     /locus_tag="VLI90_11610"
     CDS             complement(3536..>4025)
                     /locus_tag="VLI90_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14900.1"
                     /translation="SQAAAHIFASSEPPLDRVAADALAGFAPDGTMLAYTPRTSSMWG
                     PNGTGSILNFCGEYYLFVLSNKPITPEEFRDDNASSFFPLRLIADPEQIAADVARQPQ
                     AKRVIVWDRLEVDEPSSPPDDVLLKLGSKWHAEPTQLYPVRAFWDWGTMYTYRRRAYV
                     RS"
BASE COUNT          741 a         1173 c         1340 g          771 t
ORIGIN      
        1 cacgcccgat gacgacgctc accgcgccgg cgaacgcgag gtcaaggacg aactcacgtt
       61 caaaggcggc acgttcgctt cggccgcgtt cgcgaagcgc ggcttcgacc ctggtcccta
      121 tgacgaggaa atccaccccg gcagcgtggg cgggttcagc gcgatccaga acaacaaatc
      181 cggcgatggc aatatcaaat ggaacggctt cgtcgccgcc ggggagctgt cggggacgct
      241 ggtgtggacg aagaaggatg ggacggtttt gaattacacg tttaagggaa gtaaggagtg
      301 agtgtgaaaa acccgaatga cgaaagtgcg aatccccgaa tgaaatccga ataacgaagc
      361 acgaataacg aacggatgca ctaaccgctg aagcggttag tccgggcaga gcctcgcgcg
      421 agtttcgtca ttcggatttc gtcattcgtc attcattcgg gaattcgaac ttcgttattc
      481 ggttttccgg cgcaccgccg gcttcattcc cccgcctgcg cgtgttgtag caggtcggtc
      541 gggcgctgcg ggttcacata cccgcctctg gcggcgtcga aggcttcgaa cggcatgtcg
      601 tacatctggc cggtgcccgg gtctttcatg cgttcgtagc cgagcgccgt ttcttcccac
      661 ttctggctca tgcggtcctg gctggcggcg cgggtttgat acgcggacat cacgctcgtg
      721 tcgtccagcg ggcggttctt gggcaggatg atcttgtcct gcatcgccac ctgctgcggg
      781 ttggtgatgc tgacgttctc ggcgatggcg ggcagccagg tcttcacttt gtcccaatct
      841 tccggcaccg aggcggcgcc ttgcaggaac gcggtgtagt tgttgaagcc ggtgacgacc
      901 gcgcaggtca tcagcccgtg atacgcggtg ccgttcacgt cgtaggtcag ctccatctgc
      961 tgcgtcttca tcggcgcgcc ggcgtacatc tggtcgggca gatcgcgaac cgcggtgatc
     1021 tggatgttct tcgcgccgca gcgcggcagg aagccctgga cgaacccctg cgggttggtg
     1081 gtgccgggcg tgttgaccag cagacagctg gtggcgccgg cgcgaccgtc gggcgagatg
     1141 acatcgacac cgttggtcgt ctcgttgctg cgccaacctt cgggctgcga ccagcggaag
     1201 tattgcccct gctgaacgcg aagcgtcggg cgaacgtacg cgggtgcaac aggcaccggc
     1261 ggtggcgcgg ggggtgtgct gctgacacct gacggcggcg tcgtcgccga cgccagcggg
     1321 ctgttggcat cattgccggc gttcgacatg gcggattcac ccagcggatt ggacgcgtca
     1381 gtcccctgcg gcgtggccgg ggcgaccagt gggttgtcgg cgccggggtt cgccatcgac
     1441 aggtgataca gcgtcgtgcc ggtcttgaag ttcagctgac ccttggccgg cacacccatc
     1501 gcgatctgat aggttttccc gttggccgtg aacgagccaa acatcttcat cggatcgttc
     1561 ttgtcggcgg tggctttgag cgggaaggtc tggccattga gcgtgacggt gccgctgatg
     1621 ttattcccga cgcgggtgag cgtgacgctg agctttccat cggtgtacgt cgcggcatgc
     1681 gctgcggcag cgggcggatt ggccgcgctg gcgacgacgg cggcggtcag cagcaggatg
     1741 cacagccaga gaatcgtctg tccccactgg ctcgtgtcga acccatcatc gtgcttgcga
     1801 ctggcattgc gatcgcgctc catgacacac ctctgcgaaa acaaacgtgc gattgacgaa
     1861 tggcccgtga cgacacgggt ggtcatagaa agaaacgccg acatgtgaac ctccttgcgc
     1921 tgttacaaac gccgattggg cgtataacgc ggatgtgacc gtgaccctga atacgttgcg
     1981 gcaggacgtg gagtcctgca cgcgttgtcc ccgactggtg acataccgcg cggcggtggc
     2041 ggttgccaag cgccgcgcgt ttgccgactg ggactactgg ggaaaacccg ttgccggctt
     2101 cggtgatccc gccgcacgat tgtggatcat cggcctggcc cccgcagccc atggggccaa
     2161 ccgcacgggc cggatcttca ccggtgaccg cagcggtgac tttctattcg cggcattgta
     2221 ccgatgtggg ctggcaaatc agcagaaaag tgtttcgcgc gaagatggat tggcgttgtc
     2281 agattgtttc atcagcgcca cggccagatg cgccccgccg gcgaacaaac cgttgccgga
     2341 ggaagtggag aactgctcgg ggtacctgga ccaagagtgg cggctgctgg gcaagaagcg
     2401 cgtgatcctg gcgctgggcg ggatcgcgtg gaatgcatcg ctgacgctgg cggcgcgcaa
     2461 tggctgccag attgcccgcc cgcgaccggc gtttgggcat ggagcggagc tgcagctttg
     2521 ccccggactg acgctggtgg gttcgtatca tgtcagtcag cagaacacgt ttacgggaag
     2581 gttgacgccg gcgatgttcg atggcgtgat caagcgcgcg atggcgctct gtcgttcaac
     2641 caggaagccc cgcatgtacg tgccgggctc cggtgcgacg tagaacgcta ttgcttgtag
     2701 ccgcgcgacg cctgcgccgg ctctggtggc tcggaaaacg cgggacgcag gtgtcgcaac
     2761 tactcgccca ggtccgtcat tccgccgctg agttggagtt cgacgatctg catcaactgc
     2821 tcgggatcga caggtttaag cagcaatggc ggcagctcct gctcgcgcag gcggtgcatc
     2881 ttgtgttgaa tctcatcgac cggcatcccc gacaacagca ccaccggcag cgatcggcgg
     2941 tgcgattgaa tgtaattcag cacgctgaac ccatcgccct gcggcatgtt cagatcgagc
     3001 agcaacaggt ccacctgcgt gttcttcaga tgaaggaccg ccgagttgcc gtcgccggct
     3061 tcgatcacgt cgtagccgtt tttcctgagg tagtgcgcca gcgccaggcg ttcgacggcc
     3121 gcgtcatcga ccaccagcag ccgcgggggc ggcgctggaa ggggcgtggt ttcatcggct
     3181 tgcgcaggtt ccacctgcat gagtgtgatt agaccacttg cccgcgtcta ctggcaacgc
     3241 caattttgcg gaggcccggt ataatccgcg catgggtaag aaaaatcaga cgccgccgaa
     3301 aaacttcgag gaagcgctcg gagaactgga gcagatactg gcggacatcg agggcggcga
     3361 gatcgggctg gaagaatcga tcacgaagta cgaacgcggg acgttcctga tccaacattg
     3421 ccgcggcgtc ctgagcacgg cggagaagca gatcgaggtg ctgtccaagg gctcagatgg
     3481 tgaactacag agcgagccga tgagcgacga agccgctgag ggaagcgcgg ggtgatcacg
     3541 atcgcacgta ggcgcgccgg cgataggtat acatcgtgcc ccaatcccag aacgctcgga
     3601 cgggatacaa ctgcgtgggt tcggcgtgcc acttcgatcc cagcttgagc agcacatcgt
     3661 ccggcggcga tgacggttcg tccacttcga gccggtccca cacgatgacg cgtttcgcct
     3721 gcggctgtcg agcgacatcg gcggcgatct gctcgggatc ggcgatcaaa cgcagcggga
     3781 aaaaactgct cgcgttgtcg tcgcgaaatt cctccggcgt gatcggcttg ttgctcagca
     3841 cgaacagata gtactcacca caaaaattga gaatcgaccc ggtgccattg ggtccccaca
     3901 tcgatgacgt acgcggcgtg taggcgagca tcgtgccatc gggcgcgaat ccggcaagcg
     3961 catccgccgc cacgcgatcg agcggcggtt cgctggatgc gaagatgtga gcggccgcct
     4021 gcgag
//
LOCUS       DATIMH010000667         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5074042, whole genome shotgun sequence.
ACCESSION   DATIMH010000667 DATIMH010000000
VERSION     DATIMH010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5074042"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..1245)
                     /locus_tag="VLI90_11615"
     CDS             complement(283..1245)
                     /locus_tag="VLI90_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HSV14901.1"
                     /translation="MKQISFADAEYAGKRKKTRREVFLDEMEQVVPWKALLQVIEPFY
                     PMAGNGRRPYPLATMLRVHLMQNWFALSDPAMEEALYEIASLRNFAGLDLRKAIPDET
                     TILNFRHLLEEYDLAEGILKQVNAHLTRKGLLLKRGSIVDATIIAAPSSTKNAEGERD
                     PEMHQTKKGNQWHFGMKAHIGVDADSGLVHTVTTTPANESDVEQVADLLHGKEKHVYA
                     DSGYRGAASRVGGEDLQWHIAARPSDIAKLPEGRAKVRMQKQEHRKASVRAKVEHPFR
                     VIKRQFGLVKVRFRGLAKNRAHVITLFALSNLWMARRRLMAMTA"
BASE COUNT          251 a          509 c          451 g          321 t
ORIGIN      
        1 cacgctgttc gcgctgtcga acctgtggat ggcgcggcgg cgactgatgg cgatgacggg
       61 atagttgcgt ccgcagcacc cgaaaggggc gcgtacaaga ccccgttggg ggatcgcgcg
      121 accgaacacg gcgattcaac acagaaatgc tccacgaaga cgacggagtg atcagaacaa
      181 tcgccgtctt cgtcgagcat ttcgggtttt gaatcgccgt gttcggtcgg gcgacccagc
      241 aacaagctct tgtacgcgcc ctttggggcg ctgcggacgc aactatgccg tcatcgccat
      301 cagtcgccgc cgcgccatcc acaggttcga cagcgcgaac agcgtgatga catgggccct
      361 gttcttcgcc aagccgcgaa agcgtacctt caccagaccg aactggcgct tgatcacccg
      421 aaacgggtgc tcgactttcg cgcgcacgct ggccttgcgg tgctcttgct tttgcattcg
      481 cactttcgct ctgccctcag gcagcttggc gatgtcactg ggtcgcgctg cgatgtgcca
      541 ttgcaggtct tcgccaccga ctcggctcgc ggcgccgcga tagcctgagt cggcatagac
      601 atgcttctcc ttcccgtgca gcagatcggc cacctgctcc acatcggact cgttggccgg
      661 cgtcgtggtg accgtgtgga ccaggccact gtcggcatcc acgccgatgt gcgccttcat
      721 cccaaagtgc cactggttgc ccttcttcgt ctgatgcatc tccggatcac gctcgccctc
      781 ggcgttcttc gtcgaactgg gcgccgcaat gatcgtcgca tcaacgatgc tgccgcgctt
      841 gagcagcaag cctttgcgcg tcaggtgcgc attgacctgc ttgaggatgc cctcggccag
      901 gtcgtactcc tccagcaggt gccggaagtt caggatcgtc gtctcgtccg ggatcgcctt
      961 gcgcaagtcc agcccggcaa agttgcgcaa cgacgcgatc tcgtacagtg cctcctccat
     1021 cgccgggtcg ctcaacgcga accagttctg catcaggtgc acgcgcagca tcgtcgccag
     1081 cggatagggc ctgcggccat taccggccat cgggtagaac ggctcgatca cctggagcag
     1141 cgccttccac ggcacgacct gttccatctc gtcgaggaac acctcgcgcc gggtcttctt
     1201 ccgcttgccc gcgtactccg cgtccgcaaa actgatctgc ttcatcgatt tgagccctcc
     1261 gcccgattca acgcttcacg gcagaatcgg tggacttgtt cagaccttcc ctaacctggc
     1321 gttcaaccgg acgcccgcgt acgtgtcttc gttctcccga gcgccggtgg gcgggcaccg
     1381 gttaacttaa tcgttaggtc cgacgagggg cacgcatgag gtcgcttgag ttgatggttc
     1441 ctccgccggc agtcgcagcg gttgtcgcgc tcgctatgtg gggaatctcg ttggtcacgc
     1501 cgccgctgta catgccggca gcggttcgcc tc
//
LOCUS       DATIMH010000668         5301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3912264, whole genome shotgun sequence.
ACCESSION   DATIMH010000668 DATIMH010000000
VERSION     DATIMH010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3912264"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..2325
                     /locus_tag="VLI90_11620"
     CDS             232..2325
                     /locus_tag="VLI90_11620"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV14902.1"
                     /translation="MAEVTIEQAMEIAVAHHQAGRLAQAEPIYRQVLSKVPNHVDALH
                     LLGMIALQSGKTDPAIDLIRRAIAINPRVPDYYNNLGMALLSKPDYPAALSAFRSAIS
                     LRAEYPQAHNNYGVALAASGQAPAAIESYRTAIRLKPDYVEAHRNLADSLNSQGQFAE
                     AVESYRAALRCAPNDARTLNDLANALRQVGKQDEAEATYRQSIANAPNFAVPYNNLAN
                     LLKDQGHLEQAIEFYRKAIEISPKIAPLYNNLGNALAETGALDEAIVSYRKVLELTPQ
                     DSALHGSLLLTLHYHPDIDPKTNLEEHRIWNQRHGEPLKKFIQPHRNDRDPNRRLRIG
                     YVSADLRDHPVGRFLLPLLQQHDRAQFENFCYAGAPVPDATTEQIRAVADVWRPTLTL
                     SDEQLAEQIRKDQIDILVDLAAHTAGSRLRVFAMKPAPVQVSYLAYVGTTGLEAIDYR
                     LTDPYLDPPGLNDEFYAEESIRLPETYWCYQPAGAAPEPGPLPALAAGHVTFGSMNNF
                     AKLNDRVLDTWAQLLKTVPNSWLLIHAKAGSHRTRVENRMAADGIDTNRIGFINRLPI
                     EQYFSQYQRFDIALDTYPYGGGTTTCDALWMGVPVVSRFGQRAVSRAGLSILSNAGLP
                     HLVAETDERYVQIAAELTADLPKLAELRATLRDRLRSSPLMDAPRFARNVENAFRTMW
                     RRWCEEKRKAQATPS"
     gene            2439..3707
                     /locus_tag="VLI90_11625"
     CDS             2439..3707
                     /locus_tag="VLI90_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 6 family protein"
                     /protein_id="HSV14903.1"
                     /translation="MKSTLSGFLLMLAIGCSTQGSEPRALSQVETTHHPGDVIVLPDG
                     TYKDQNLHLTGVGTADHRITFLAQTPGKVVFTGNSVITLDGQYLVLSGIMLKNGGAGS
                     STKGKGEPDDGIAVKGAHNRLTDCAVSGGTYKFLVHLYGADIHMDHCYLAGKTSGEPT
                     LQIEAPGEPNNDHIEYNHFGHRRPLGRNGGETMRVGYSWQSMNNSRSLVEHNLFDRCD
                     GEIEIISSKSCENTYRYNTFLDCGGMLTLRHGNRCVVDSNFFIAHHKKGSGGIRVIGE
                     DHVIVNNYIEGVEQGGFWITSGLEHPPLKTYFAARNCVIAFNTFVDSAGPAVDLDAGF
                     GSSNRTVMPQDITIANNILSPDQNGAVLKGKAGPGYAILGNLSAKDASESNITGIRAA
                     DLGLQRGSDGLWRPTANPTSAVTRLDRPWR"
     gene            complement(3740..>5301)
                     /locus_tag="VLI90_11630"
     CDS             complement(3740..>5301)
                     /locus_tag="VLI90_11630"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HSV14904.1"
                     /translation="TRRFGDVWLGWRLWQALELDRFCAAQMPAGREQVAWATMAAILV
                     IARLCEPSSELHIAEDWYRRTALADLAGVAVERVNDDRLYRALDRLLPHKRGLEEHLK
                     RRLGELFALDYELLLYDVTSTYFEGLCESNPLARRGYSRDQRFDCKQVCIALVVSRCG
                     MPIGYELFAGNKADVATVEQIVTTMEDRYGKSDRIWVMDRGMSSADNIEFLKQEKRRY
                     IIGTPKSMLKKYERELLKDDWSLVRDGLEVKLCAAPEAKESESNETSDETSDETSDET
                     SHETSHETSHETFILCRSSDRAGKERAMHERFEKRIEEGLSRIVNACHRQKLDAVKVS
                     HRVGKLLGQNTRAAGAFKTKIETDEQGRAMLKWEKIESWRDWSRLCEGCYLLRSNVRD
                     WNAEELWRAYMQLSEAEGAFRIHKSDLSLRPVWHQKQQRVEAHVLVCFLAYVLWKTLA
                     QWCKQAGLGDEPRKVLDEIAQVALVDVVLATKDGREIVKRCVAQASEHQKLLLHRLKL
                     HLPTHLEAWPM"
BASE COUNT         1008 a         1813 c         1528 g          952 t
ORIGIN      
        1 ggtggttggt ttcatgtcga acatatccta gcgcgagacg cgcgatgggt aaagcaatca
       61 atgttgtcac tattcactat gtcctttgcg tagcggtaga gatcgcaggg gcacgtgttt
      121 tttctttcga gagcgaaaac acgtgcccct gcggggctac cgctacgcat ggcgtccaaa
      181 agccgcgcgg cgcattgcat ccaacgccac tcccggccct aatgcaccgc gatggcggag
      241 gtcacgattg aacaagcgat ggagatcgcc gtcgcccacc accaggcggg gcgattggcg
      301 caggcggagc ccatttaccg ccaggtgctc agcaaggtgc ccaaccacgt cgacgccctg
      361 cacctgctcg gcatgatcgc actccaatcg ggcaaaactg atcccgcgat cgatctcatc
      421 cgccgggcga ttgccatcaa cccgcgcgtg cccgactact acaacaacct gggcatggcg
      481 ctgctcagca agccggatta ccccgccgcc ctgtcggcgt ttcgctcggc catctcgctg
      541 cgcgccgaat atccgcaggc ccacaacaac tacggcgtcg ctctggccgc ttccggccaa
      601 gctcccgcgg ctatcgaatc gtatcgcacc gccatccgcc tcaaacccga ctatgtcgag
      661 gcccaccgca atctcgccga ttcgctgaac tcgcaaggcc aattcgccga ggcggtcgag
      721 tcgtatcgcg ctgcgcttcg ctgcgcgccg aacgatgcgc gcacgctgaa cgatttagcc
      781 aacgcgctgc gccaagtcgg caagcaggac gaggcggaag cgacctatcg ccagtcgatc
      841 gccaacgccc ccaacttcgc cgtcccctac aacaacctcg ccaacttgct gaaagaccag
      901 ggccacctcg aacaggcgat cgagttctac cgcaaggcga tcgagatcag cccgaagatc
      961 gccccgctgt acaacaatct cggcaacgcg ctggccgaga ccggcgcgct cgacgaggcg
     1021 atcgtcagct atcgcaaggt gctggagctg acgccgcagg attcggccct gcacggcagc
     1081 ctgctgctga cgctacatta ccatccggac atcgacccga agacgaacct ggaagaacac
     1141 cgcatctgga accagcgcca cggcgagccg ctgaagaaat tcatccagcc ccaccgcaac
     1201 gaccgcgacc ccaaccgccg gctgcgcatt ggttatgtct cggccgatct gcgcgaccat
     1261 cccgtcggcc ggttcctgct gccgctgctt cagcaacatg accgggcgca gttcgagaac
     1321 ttctgctacg ccggcgcgcc ggtgccggat gcgacgacgg agcagatccg cgccgtcgcc
     1381 gacgtttggc ggccaacgct cacgctgagc gacgagcagc tggccgagca gattcgcaag
     1441 gaccagatcg acatcctcgt cgatctcgcc gcccacacgg ccggcagtcg attgcgcgtc
     1501 tttgcgatga agccggcgcc ggtgcaggtg tcgtatctcg cgtacgtcgg cacaaccggt
     1561 cttgaagcga tcgactatcg gctgacggat ccgtatctgg acccgccggg gttgaacgat
     1621 gagttctatg ccgaggaatc gatccgcctg ccggagacgt actggtgcta tcaaccggcc
     1681 ggggcggcgc cggaaccggg accgctgccg gcgctggcgg cgggtcatgt cacgttcggc
     1741 tcgatgaaca attttgccaa gctgaacgac cgcgtgctcg acacgtgggc ccagctgctg
     1801 aaaaccgtgc cgaactcgtg gctactgatt cacgccaagg ccggcagcca tcgcacgcgc
     1861 gtcgaaaacc gcatggccgc cgacgggatc gacacgaatc gaatcggctt catcaaccga
     1921 ttgccgattg agcagtattt ctctcagtac cagcgcttcg acatcgcgct ggacacctat
     1981 ccgtacggcg gcgggacgac gacctgcgac gccctgtgga tgggtgtgcc ggtggtcagc
     2041 cggttcggtc agcgcgcggt gtcgcgcgcg gggttgagca tcctgtccaa cgcaggcttg
     2101 ccgcacctgg tggccgagac cgacgaacgc tacgtgcaga tcgccgcgga gttgacggcg
     2161 gatttgccaa agctcgcgga gctgcgcgcg acgctgcgcg atcgcctgcg ttcatcgccg
     2221 ctgatggacg ccccgcggtt cgcgcgcaac gtcgagaacg cctttcgaac gatgtggcga
     2281 agatggtgcg aagagaaacg caaagcccaa gcaactccat cgtgacacgg gcctccggcc
     2341 tgtgcgcgca ccaagcgact ttcaggaact tcagtttctt ctacttccaa ctcgctcgca
     2401 cgggccagag gcccgtgtca cggtgaggca ttagccatat gaagtcaacg ctctctggtt
     2461 ttctgttgat gttggcaatc ggttgctcaa cgcagggttc cgagccccgc gcgctttcgc
     2521 aggttgagac aacgcaccac ccgggggatg tgattgtcct tcccgacgga acgtacaagg
     2581 accagaacct gcacctgacc ggcgtcggca ccgccgatca tcggatcacc tttctcgccc
     2641 aaacgcccgg caaggtcgtc ttcaccggca actccgtcat caccctcgac ggccaatacc
     2701 tcgtcctcag cggcatcatg ctgaagaacg gcggcgccgg cagctccacc aagggcaaag
     2761 gcgaacccga cgacggcatc gccgtcaaag gcgcccacaa ccgcctcacc gactgcgccg
     2821 tcagcggcgg gacgtacaaa ttcctcgtcc acctgtacgg tgccgacatc cacatggacc
     2881 actgttacct ggcgggcaaa accagcggcg aaccgacgct gcagatcgag gcgccgggcg
     2941 agccaaataa cgaccatatt gaatacaacc attttggcca tcgccggccg ctcggccgca
     3001 acggcggcga gacgatgcgc gtcgggtaca gctggcaatc gatgaacaat tcgcgctcgc
     3061 tggtggagca caacctcttc gaccgctgcg acggcgaaat cgaaatcatc tccagcaaat
     3121 cctgcgagaa cacctatcgg tacaacacgt tcctggactg tggcggcatg ctcacgctcc
     3181 gccatggcaa ccgctgtgtc gtcgattcga acttcttcat cgcccatcac aagaaaggct
     3241 cgggcggcat ccgggtgatc ggggaagatc acgtcatcgt caacaactac atcgaaggcg
     3301 tggagcaagg cggattctgg atcaccagcg ggttggaaca tccgccgctc aagacctact
     3361 tcgccgccag gaactgcgtc atcgcgttca acacgttcgt cgattccgcc ggcccggcgg
     3421 tggacctcga cgccggcttc ggcagcagca accgcacggt gatgccgcag gacatcacga
     3481 tcgcgaataa tattttgtcg ccggaccaga atggcgcggt gctgaagggc aaagccggcc
     3541 cgggctacgc gatcctcggc aacctctcgg ccaaagatgc gagcgagtca aacatcaccg
     3601 gcatccgcgc cgccgacctc ggcctgcaac gcggcagcga cggcttgtgg cgaccaacgg
     3661 ctaacccaac ttccgcagtt acgcgtttgg atcggccttg gcggtagcgc cagtgctgat
     3721 tcggggtcgg ttttaggcac tacattggcc acgcttccaa gtgagttgga aggtgcagtt
     3781 tcaagcgatg cagcagtagt ttctgatgct cgctcgcctg agcgacgcat cgcttgacga
     3841 tttctcggcc gtccttggtc gccagcacca cgtccaccag tgcaacctgt gcgatttcgt
     3901 ccagcacctt ccgcggctca tcgcccaggc ccgcctgctt gcaccattgg gccagggttt
     3961 tccacagcac gtacgccagg aagcagacca gcacatgcgc ctcgacgcgc tgctgcttct
     4021 ggtgccagac cgggcgcagg ctcaggtcgc ttttgtgaat acggaacgcg ccctcggctt
     4081 cgctcagctg catgtacgcc cgccagagtt cttcggcgtt ccagtcccgc acgttgcttc
     4141 gtagcagata gcagccttcg cacaagcgcg accagtcgcg ccaggattcg atcttctccc
     4201 acttcaacat cgcgcggcct tgttcgtcgg tctcgatctt cgtcttgaac gccccggcgg
     4261 cacgggtgtt ctggcccagc agttttccca cccggtgcga caccttcacc gcatcgagct
     4321 tctgccgatg acaggcattg acgatgcgac tcagcccctc ttcgatccgc ttctcgaagc
     4381 gctcgtgcat cgcgcgttct ttgccggcgc gatcgctcga tcggcacagg atgaatgtct
     4441 cgtgcgacgt ctcgtgcgac gtctcgtgcg acgtctcgtc tgacgtctcg tctgacgtct
     4501 cgtctgacgt ctcgttcgac tcactttctt tggcctcggg cgcggcgcaa agcttcacct
     4561 ccagcccgtc gcgcaccagc gaccagtcgt ccttcagcaa ctcgcgttcg tactttttca
     4621 acatcgactt gggcgtgccg atgatgtaac gccgcttctc ctgcttcagg aactcgatgt
     4681 tgtcggcgct gctcatgccg cggtccatca cccagatccg atcggatttg ccgtagcgat
     4741 cctccatcgt cgtaacaatc tgttcgacgg tggcgacgtc ggccttgttg ccggcgaaca
     4801 gctcgtatcc gatcggcatg ccgcagcggc tgaccaccag cgcgatgcag acctgcttgc
     4861 agtcaaaacg ctggtcgcgc gaatagccgc gccgggccag cgggtttgat tcgcacagac
     4921 cttcgaagta ggtgctggtc acgtcgtaga gcagcagctc gtagtcgagc gcgaacagct
     4981 cgcccaagcg ccgcttgaga tgctcctcca gcccccgctt gtgcggcagc agccggtcga
     5041 gcgcgcggta cagacggtcg tcgttgaccc gctccaccgc caccccggcg agatccgcca
     5101 gcgccgtccg ccggtaccaa tcctcggcga tgtgcagctc gctcgacggc tcgcacagcc
     5161 gcgcaatcac caggatcgcc gccatcgtcg cccacgccac ctgctcgcga cccgccggca
     5221 tctgcgcggc gcaaaaccga tcgagctcca gcgcctgcca cagccgccag cccagccaga
     5281 cgtcgccaaa acgccgcgtc c
//
LOCUS       DATIMH010000669         3860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_161341, whole genome shotgun sequence.
ACCESSION   DATIMH010000669 DATIMH010000000
VERSION     DATIMH010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161341"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1178
                     /locus_tag="VLI90_11635"
     CDS             96..1178
                     /locus_tag="VLI90_11635"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HSV14905.1"
                     /translation="MALTTNSNDSAGTGANGIAGASTTTTNGSLATLRHVLEFEKPLA
                     KLEQQILELEALQVAKQTDYSKELRQLRTNYTSLLRKTYDNLSAWETVQVARHPQRPL
                     FRDYLDMICREFREIHGDRLFGDDRAIMCGLARMGGHKVMLIGHHKGRDTKEKVKCFF
                     GLAHPEGYRKALRCMKLAEKFGLPVVTLIDTPGAYPGIGAEERGQAESIARNMFEMSR
                     LKTPILSIVTGEGGSGGALGIGVADRVAMLEFAWYSVISPEACSAILFKGNEGAPQLA
                     DSLKLTSKHLKHLGIIDTIVPEPLGGAHRDPHTAAHSLDQYVAKTLRALKSTKIENLL
                     ERRYERFRNLGVVIEKSLKQNIKAAG"
     gene            1444..>3860
                     /locus_tag="VLI90_11640"
     CDS             1444..>3860
                     /locus_tag="VLI90_11640"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HSV14906.1"
                     /translation="MGNMVFAHRRSPRPSIDRHALRPAAEVIEPRRLLSAFTVNTTSD
                     VTNPSDGLTTLREAIVAANSHGGADTINFSPAVFTSGTLHTIRLTAGQLSITDTSGTT
                     TISGPGSSVLAVDAQQKSRVFQINANVTAAISGLTITGGLITAPTGATPANGGGIFTA
                     GHLSLSHVVVDANEVVVPFDFTTAATAAAASGGGIYSSGTLSITSSTISGNEAVGGST
                     SLYPNGAAKGGGIYSAGALELATSTIINNIAAGGVPQANTGQHGGDAFGGGIYCSASL
                     TVTQSSLTNNIALAGTSAFPNSTVGGNAKGGAIFALANCSLSDSTLTGNSATGGDSGY
                     AGTSTVDSDPNTLDSASGGGAYCAGNTLSVLRCTFSNNRAVAGNGASGESGPGGNAAG
                     GALVTLANTSLASTTISGNLAQSGDGDIPPTTVYGGGIDARASLTITACTITGNTALG
                     GTGGYYNGVGAAALGGGVHAAGTLLVTASAISGNHTIGGRGGEGGLFVQGGAGPGGLG
                     AGGGIYSDAGATITGTTIANNDATGGAGGQGLGFQNAADNGGDGAGGAVYGLDPLSIA
                     ASTLSGNTASGGKGGTANSYSEPDSSRVAGLGGNGSGGAVFADGDPTIVNATLANNIA
                     VGGDGGDLRHTFAGQANSGGNGFGGAVDGGHAFTLANSTVSGNRAAGGNAGYTPPGAV
                     DPIIAKAGHGSGGGLWINNGAPVLTNSIVSANTRDGAAFDDVRGSLNKTSSKFNLIGV
                     GGGLTNGVNGNKVSVNNPQLQALGNYGGPTQTMRPIWPSSPAIDAGSNALIPAGVTTD
                     QRGQPRIA"
BASE COUNT          752 a         1319 c         1167 g          622 t
ORIGIN      
        1 tggagcggag gggccacgtt ggctcgtggg gattgtccta taaggtgaaa gaggctggcc
       61 gcgggatccc tccccgccgc ggtcggaagg tggatatggc actgactacg aactcgaacg
      121 actcggccgg taccggtgcg aatggaatcg cgggtgcctc gacgacgacg accaacggct
      181 ccctcgccac gctgcgccac gtcctggaat ttgaaaagcc gctggccaag ctcgagcagc
      241 agatcctgga gttggaggcg cttcaggtcg ccaagcagac ggactactcc aaggagctgc
      301 gccaactccg caccaactac acgagcctgc tgcgcaagac ctacgacaac ctcagcgcct
      361 gggagaccgt gcaggtcgcc cggcatcccc agcggccgct gttccgcgac tatctcgaca
      421 tgatttgccg ggagttccgc gaaatccacg gcgatcgact cttcggtgat gaccgcgcca
      481 tcatgtgcgg cttggcccgc atgggtgggc acaaggtcat gctcatcgga catcacaagg
      541 gccgggatac caaggaaaag gtcaagtgtt tcttcggtct cgctcacccc gaaggctatc
      601 gcaaggcgct tcgctgtatg aagctggccg agaagttcgg actgcccgtc gtcacgctga
      661 tcgacacccc cggcgcttac cccggcatcg gcgctgaaga acgcggccaa gctgaatcga
      721 tcgctcggaa catgttcgag atgtcccgcc tgaagacgcc catcctcagc atcgtcaccg
      781 gcgaaggcgg cagcggcggg gcactgggca tcggcgtggc cgaccgcgta gccatgcttg
      841 aattcgcctg gtacagcgtc atcagccccg aagcctgcag cgcgatcctc ttcaaaggca
      901 acgaaggcgc gccgcagttg gccgacagcc tgaagctgac gagcaagcac ctcaagcacc
      961 tcggcatcat cgacacgatc gttcccgagc ccctcggcgg cgcccaccgc gacccgcaca
     1021 ccgccgccca tagcctcgat caatacgtcg ccaagacgct gcgtgctctg aagagcacga
     1081 agattgagaa cctattggaa cgccgctacg agcgcttccg caaccttggc gtggtgatcg
     1141 agaagtccct gaagcaaaac atcaaagccg cgggataaca gatccccatt cgctgttcac
     1201 gttcctagag caggttgcac ctgctcgtag cggcgcaagc tctcaggatg acaattacaa
     1261 cgccacgatc aggtgcaact cgctctaatc atcgctattg cgcgacatgc ctgattctgc
     1321 agcgccccgg atatgaactc tgggtcacat gatcgcatgc cagccatttt ctggttgaca
     1381 gaactgcgcc cccgcctcag cataacgtgc cggaaacgct tatcaaaggc ggggacaacc
     1441 atcatgggaa atatggtttt tgcccatcgg cgatcaccac gtccatcgat cgatcgccac
     1501 gcgctgcgcc ccgcggctga ggtgatcgag ccgcgtcgat tgctgtcggc cttcaccgtc
     1561 aacaccacca gcgacgtcac caatccatcc gatggcctga ccacgctgcg cgaagcgatc
     1621 gtggctgcca actcgcacgg tggggcggac acgatcaact tcagtcccgc cgtcttcacc
     1681 agcggcaccc tgcacacgat cagattgacc gctggacaat tgtcgatcac cgacaccagc
     1741 ggcacaacga cgatcagcgg accgggatcg agcgtgctgg cggtggatgc ccagcagaag
     1801 agccgcgtct tccagatcaa cgcgaacgtc accgccgcta taagcggttt gacgatcacg
     1861 ggcggcttga tcacagcgcc gactggcgcg acgcccgcca acgggggtgg catcttcacc
     1921 gccggtcacc tgtcgctcag ccacgttgtg gtcgatgcca acgaagtcgt cgttcccttc
     1981 gacttcacca cggcggccac cgccgccgcg gctagcggtg gtgggatcta cagctccggc
     2041 acgttgtcga tcaccagcag caccatctcc ggcaacgagg ccgtcggtgg ttcaacaagc
     2101 ttgtacccca atggcgccgc gaagggcggt ggcatctaca gcgccggcgc gctggaactc
     2161 gccaccagca cgatcatcaa caacatcgcg gccggcggtg tgccgcaggc caacactggc
     2221 caacacggcg gcgatgcctt cggcggcggc atctattgca gcgcttcgct caccgtcacc
     2281 caatcctcgc taacgaacaa cattgccctg gctggaacgt ctgcgtttcc aaattccacg
     2341 gtcggcggca acgccaaggg gggtgcgatc ttcgccttgg ccaactgttc gctgagcgac
     2401 tccaccctca caggcaattc cgcgaccggt ggcgacagcg gatatgccgg cacgtctacc
     2461 gtcgatagcg atccaaacac gctggattcc gccagcggtg gcggcgcgta ctgcgccggc
     2521 aacacgctgt cggtgctgcg atgcacgttc agcaacaatc gcgccgtcgc cgggaacggc
     2581 gcctctggcg aaagtggtcc gggcggcaac gccgccggtg gcgcgctggt cacgttggcg
     2641 aacacgtcat tggccagcac cacgatctcc ggcaatcttg cccaaagcgg tgatggcgat
     2701 ataccgccga ccaccgtcta cggcggtggc atcgacgctc gcgccagtct gactatcacc
     2761 gcctgcacga tcaccggcaa taccgccctc ggtggcaccg gcggttacta caacggcgtt
     2821 ggcgcggcgg cgctcggtgg tggcgtgcat gccgctggca cgctgctcgt caccgcctcc
     2881 gcgatctccg gcaatcacac catcggtggt cgtggcggcg aaggaggact cttcgtgcaa
     2941 ggcggcgcgg gccccggcgg tctgggcgcg ggtggcggca tctacagcga tgccggcgcg
     3001 acaatcaccg ggaccaccat tgccaacaat gacgccaccg ggggcgcagg cggccagggc
     3061 ttgggattcc aaaatgccgc cgataacggc ggcgacggcg cgggtggcgc agtttatggg
     3121 ctggaccccc tctctatcgc cgccagtacc ctctccggca ataccgcgtc tggcggcaag
     3181 ggcggcacgg ccaactccta ttctgagccc gactcctccc gagtcgccgg cctcggtggt
     3241 aacggttccg ggggcgcggt cttcgccgat ggcgatccca ccatcgtcaa cgccaccctc
     3301 gccaacaaca tcgccgttgg cggcgacggc ggcgatctcc gccacacctt tgccggccaa
     3361 gccaacagtg gcggcaacgg atttggcgga gcggtggacg gcggtcacgc cttcacgctc
     3421 gccaacagca cggtatccgg caatcgcgcc gccggcggca atgccggtta cacgccgccc
     3481 ggcgccgtcg atcccatcat cgcgaaggcc ggccacggca gcggtggtgg gttgtggatc
     3541 aacaacggcg cgcccgtgct caccaactcc atcgtctccg ccaacacgcg cgatggtgcc
     3601 gcgttcgatg acgtccgcgg ctcgctcaac aagaccagca gcaaattcaa cctgatcggt
     3661 gtcggcggcg gcttgaccaa tggcgtgaac ggcaacaagg tcagcgtcaa caacccgcag
     3721 ctgcaagcgc taggcaacta cggcggcccg acgcagacga tgcggccgat ctggcccagc
     3781 agccccgcca tcgacgccgg cagcaatgcg ctaattcccg caggcgtgac gacggaccaa
     3841 cgcggccagc cgcggattgc
//
LOCUS       DATIMH010000670         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1029674, whole genome shotgun sequence.
ACCESSION   DATIMH010000670 DATIMH010000000
VERSION     DATIMH010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029674"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VLI90_11645"
     CDS             complement(<1..305)
                     /locus_tag="VLI90_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSV14907.1"
                     /translation="MSFAAGEKLGPYEFLAPIGSGGMGEVYCARDTKLDREVAIKVLP
                     EALVRDADRLARFQREAKALAALSHPNICTIHDVGEDRGIPYIAMELMEGRTLQERIG
                     "
     gene            complement(702..1865)
                     /locus_tag="VLI90_11650"
     CDS             complement(702..1865)
                     /locus_tag="VLI90_11650"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HSV14908.1"
                     /translation="MTRPPFEVADITRAPGKNFIDRNRSWLRGGHLKVLSAIERCRTA
                     ALGGHRDRCLTCGYETTSYSFNSCRNRHCPKCQTNAREKWLAKRSADLLPVPYVHVVF
                     TLPHLLVPLAYHNKKLTYDLLFRASAATLLEVAANPKRLGAELGFLSVLHTWGQTLEH
                     HPHVHCVIPAGGLSPDRERWIPARQDFFLPVPVLRKVFRGKCTDGLKRLWREKRLCLP
                     GALKALKQEKTFRTFLRSLHGKKWVVYAKPPFGGPAHVLQYLARYTHRVAISNHRIVN
                     FAEGKVSFRWKDYAHSSQQRVMTVDADEFLRRFLQHLLPRRYVRIRSFGFLANRQRSA
                     SLALCRQLLAVAEVPVSEAKTGETAWRCPHCGGPMVVMERLTAPQLEVVLDSS"
     gene            complement(1862..>2752)
                     /locus_tag="VLI90_11655"
     CDS             complement(1862..>2752)
                     /locus_tag="VLI90_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HSV14909.1"
                     /translation="LEELQRRNYSQTTTRAYLRAVASFAQHFGKPPDQLGPDEIRPYQ
                     AHLIEERRIGVRTAANHTAALRFFYVKTLKRPYALEEVPYPKRPRRLPIVLSQEEAIE
                     LINSAKNLYHRAMLETLYSTGMRRAELCQLEVEDIDSHRMVIHIRQGKGGKDRDVPLS
                     ANLLETLRAYWRWMRPRTYLFPGTVNNWRADKPITTKNVWAACVEAARRAGITKRVSP
                     HLLRHSYATHLMENVADLLTVQALLGHSDLKHTAIYLHLSQRHLKAAGTPLDKAVLAP
                     LEQVQRPRKPLKPTPAAKRG"
BASE COUNT          532 a          810 c          867 g          543 t
ORIGIN      
        1 ccgatccgct cctggagggt gcggccttcc atgagttcca tggcgatata gggaatgccg
       61 cggtcttccc caacgtcatg aatggtgcat atattggggt gactcaacgc ggccaaggct
      121 ttcgcttcgc gctggaaccg cgcgagccgg tcagcatctc ggactagcgc ttcggggaga
      181 actttgattg ccacttcacg atcgagcttg gtatcacggg cacaatacac ctcacccata
      241 ccgcccgagc cgatgggggc aaggaattcg taggggccga gcttctcgcc agccgcaaag
      301 gacatagatg tttgaccatt ctactcctca gggaagatcg attcgctcaa gcgaacggga
      361 cagctggtaa ctcctgggtg acgttagaac gctatctgga ctgacccggc gccgcagagt
      421 tggcgtgagt ggggatgtcg accgacaggt cagtttggat agcgccgttt gaaggaaccc
      481 gctggcgccc ttatggcgat agtacagatt cccggaaaag cctgttgggg ttggtgatga
      541 gtacgacaat gacggggcgt gagcaagggc tgcggaaatc ggctccgaat ggcggagtcg
      601 agagtgtagg aataggtgaa aaacggtcgt tttgctcacg cacacacacc tggagtgggc
      661 gtgccggagc acaccgcgtt ttcgacatcc cgcgcgttcg atcacgagct gtcaaggacg
      721 acctccagtt ggggtgcggt gagccgttcc atgacgacca tcgggcctcc gcagtgcgga
      781 cagcgccatg ctgtctcacc agtcttcgcc tcagacacgg gcacttcggc aacggcgagc
      841 agttggcggc acaacgccag cgatgccgag cgttggcgat tggccaggaa gccgaagctg
      901 cggatgcgga cgtagcgtcg cggcagaaga tgttgcaaga agcgccgtag gaactcgtcg
      961 gcgtcgaccg tcatgacgcg ctgttgactc gagtgcgcgt aatctttcca gcggaacgag
     1021 acctttccct cggcgaagtt cacgatgcgg tgattggaaa tcgccacacg atgcgtatag
     1081 cgtgccagat actgcaacac gtgtgcgggt ccgccgaagg gaggcttcgc gtacaccacc
     1141 cattttttgc catgcaagga tcgcagaaag gttcgaaagg ttttctcctg tttcagtgct
     1201 ttgagggcac cgggcaaaca cagccgtttt tcgcgccata ggcgtttcag cccatcggta
     1261 cacttgccgc gaaacacttt gcgcagcacg ggcacgggca agaagaagtc ctgacgcgcg
     1321 ggaatccaac gctcgcgatc cggggaaaga ccgcccgcgg gaatcacgca atggacgtgc
     1381 ggatgatgct cgagcgtttg tccccacgta tgcagcacgc tcaggaatcc gagttccgcg
     1441 cccaagcgtt tgggattcgc cgcgacttcc aacaatgtcg ctgcgctggc gcgaaacagc
     1501 agatcataag tcagcttctt gttgtgatag gccagcggca cgagcaagtg cggcaacgtg
     1561 aacaccacgt gcacgtaggg caccggcaac agatccgcgc tgcgctttgc aagccacttc
     1621 tcgcgcgcat tggtctggca cttcgggcaa tggcgattac ggcacgagtt gaacgaatac
     1681 gaggtggttt catagccgca cgtcaggcat cggtcgcgat gtccgcccag cgccgccgtg
     1741 cggcaacgtt cgatggccga gagcaccttc aagtgcccac ccctcaacca ggatcgattg
     1801 cgatcgatga agttcttgcc gggcgcgcga gtaatgtccg ccacctcgaa gggtggccgc
     1861 gtcacccccg tttggccgcc ggcgttggct tgagcggctt gcgcggccgc tggacttgct
     1921 cgagcggcgc gagcacggct ttgtcgagcg gcgtgccggc ggccttcaaa tggcgctgcg
     1981 ataaatgcag atagatcgcg gtgtgcttca gatcgctgtg ccccaggagc gcctgcacgg
     2041 tgagcaggtc cgcaacgttc tccatcaagt gcgtggcgta actatgtcgg agcagatgcg
     2101 gactcacgcg cttggtgatg cctgcgcgcc gggccgcttc cacacacgct gcccacacgt
     2161 tcttcgtggt gatgggtttg tccgcgcgcc agttgttcac cgtgccgggg aacagatacg
     2221 tccggggccg catccaccgc caatacgcgc gcagcgtctc cagcaggttt gcgctcagcg
     2281 gcacgtcgcg gtccttgccg cccttgccct ggcgaatatg aatcaccatg cggtggctgt
     2341 cgatgtcctc gacttcgagc tggcacagct cggcgcgccg catcccggtc gaatacagcg
     2401 tctcgagcat cgcgcgatgg tacagattct tggccgagtt gatcagctcg atggcttctt
     2461 cctgactcag cacaatcggc aggcggcgcg gccgcttcgg atacggcact tcttcgagcg
     2521 catacggacg cttcagcgtc ttgacgtaga aaaaccgcaa ggcggccgtg tggttggctg
     2581 ctgtgcgcac gccgatcctg cgctcttcga tcaggtgcgc ctgatagggc cggatctcat
     2641 ccggtcccaa ctgatccggt ggcttgccga agtgctgggc gaaactggct accgccctca
     2701 gataggcgcg tgttgtcgtc tgagaataat tacgacgctg gagttcctcc ag
//
LOCUS       DATIMH010000671         4876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_161636, whole genome shotgun sequence.
ACCESSION   DATIMH010000671 DATIMH010000000
VERSION     DATIMH010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161636"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..2617
                     /locus_tag="VLI90_11660"
     CDS             128..2617
                     /locus_tag="VLI90_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HSV14910.1"
                     /translation="MNIRTTRTRRVFRDAAWCSIVIFSCLIARTAAADDGQPITDTHA
                     AGSHTFRYAPPAGSNPQHVNVAGDFNNWSNTADELKRAGDVYEITLHLPEGVHYYKFV
                     VDGKWTNDPASEADLEIDDNYGGKNSAVMIGPDGRHLPPAQPDAINAAAVAFDPADVR
                     DRNVASRELLRLSLRTQADDVSSANVLVLGENDEWRPTPMWKTETSLGFDRFGAVLTI
                     KAPSARYVFELKDGSATNYIASGKAYTSLSSAQAAAYSASMTPTFETPDWAKHAVWYQ
                     IFPERFRNGDPSNDPPNTKEWKSKWFSTLPGESGKFYNDVWSRRYGGDVQGIREALPY
                     LRQLGINAIYLNPIFEAEDMHKYDTSDYRHVDDNFGVKGDLAELASVETDDPATWRWS
                     KSDRIFLDFLDDAHRQGFKIIIDGVFNHVGRKHWAFQDVIKNGKNSKFASWFEITDWS
                     SPSKDAPFHYKAWDGNDGALPDWKKDPQLGIAHGPREHILAITKRWLAPDGDPSRGVD
                     GWRLDAANEVPHPFWVEWRKLVKSIKPDAYITGEIWSWAQPWLGGNEFDAVMNYRFAT
                     TGQDFFVNQQKAINCSQFNTRCNEIIFNYPFQVALVNQNLFDSHDTDRFASMFVNPDL
                     AYDASNRIQDNGPQYKADKPSDEQRQRMLQAVAWQMTFVGAPMIYYGDEAGMWGPDDP
                     SDRQPMLWKDLGTYDDPQEQFDQGKFDWYQRLIAIRAKLPALQTGFFHPVLIDNARGE
                     YVYARDLGSQHVYVVLNRNSRAQTVEFLAGDHDAKLIDWLSAAQSDVNAPNDGRPIVE
                     LNGNANSLDAPGGRASVQLKPYGAAILTEKE"
     gene            2654..>4876
                     /locus_tag="VLI90_11665"
     CDS             2654..>4876
                     /locus_tag="VLI90_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSV14911.1"
                     /translation="MTIPRAFRLLLSLAAILIVVWAFFDVIHRGIQRNSAQHDRPITL
                     TVLHWGDQAEDQIMEALDREFEDQHQNIRIVRINPGAGDYESKLKTMLAAGTPPDVFY
                     LRPEMLRDFASMKLIAPLDQQFAAEPKEWKDDFIPIMLNAWRYNAATDKIGDHTATLY
                     GLPKDFTTAVMYVNLDLFQKAGIPVPYKGWTWDEYEADIKKITDLSGTPAVEGRAIYG
                     GSIEIWHDTVRNIIWTYGGDFFGPGGFRDVTLDAPPAQEALQMLARTRQVAHTVYNPT
                     VVGQEGGEEFLRGGIGVLGPLGRWKVPRMKEVTAFKWDCVPLPHKTQEASMVWYTAWS
                     MSSASPHPKECYELIKYLTGAKGQILAAHLGLAVPSLKSVAYSDDFLNPPGMPKHNSQ
                     AFLDAVKVARIQQVPREPEWARICGEELQKALLLGTEKPAQMAQVVKQRWLSELDSPL
                     RQQQWQPMRWNIVLSITIGLLVTLFAALWWRARGERLGALDRAQERAGWSFIAPWVIG
                     FLALTLGPMIASLLLSFTHWSAMSPISEADSVGAANYKQLFTHDQTFLHSLNVTVYYV
                     LLAVPIGQAAALAVAMLMNLRLRSITAFRTIYFVPSVVGGAALAVLWLQLLDDQYGLV
                     NHIMRPILGLVHLRPPNWFGADYATSPPTDDAARWAIPALVIMGLWGVGSGMIIYLAG
                     LKGIPASLYEAATIDGAGPTGKFFNVTLPMLSPLIFYNLVMGIIGSFQIFTQAKIMTN
                     G"
BASE COUNT         1006 a         1592 c         1450 g          828 t
ORIGIN      
        1 aagcgactgc cgtaccgttc ttgtaaccaa acattcactg acttctcggc tagatccttc
       61 gactgcgcac gagtacatgc ttcgctcagg ataaagccat agacgacatt caaagaggcc
      121 gctgatcttg aacatacgca cgacccgaac ccgccgcgtc ttccgcgacg ctgcctggtg
      181 ctcaatcgtc atcttcagtt gcctgatcgc gcgcacggcc gcggcggatg acggccaacc
      241 catcaccgac acccacgcgg ctggctcaca cacgtttcgt tacgcgccgc cagcaggcag
      301 caacccgcag cacgtgaacg tcgccggtga tttcaacaac tggtcgaaca cggcggatga
      361 gttgaagcgc gcgggcgatg tctacgagat cacgctgcac ctgcccgagg gcgtgcacta
      421 ctacaagttc gtcgtcgacg gcaaatggac caatgacccg gcgagcgaag cggacttgga
      481 gatcgacgac aactacggcg ggaaaaactc cgccgtgatg atcggccccg acggccggca
      541 cttgccgccg gcgcagccag acgccatcaa cgccgccgca gtcgcgttcg atccggccga
      601 cgtgcgcgac cgcaacgtcg catcgcgcga actgcttcgc ctctcgctcc gcacgcaggc
      661 cgacgacgtg agcagcgcga acgtgctggt gctgggcgaa aatgacgagt ggcggcccac
      721 gccgatgtgg aagacggaaa cgtcgctcgg cttcgatcgc ttcggcgccg tcctcactat
      781 caaggcacca tcagcgcgct acgtgttcga gctgaaggac ggcagcgcga ccaactacat
      841 cgccagcggg aaagcctaca cgtcgctgag cagcgcccaa gccgccgcgt acagcgcgag
      901 catgacgccc acgttcgaaa cccccgactg ggccaagcac gccgtgtggt accaaatctt
      961 ccccgagcgc ttccgcaacg gcgacccatc caacgatccg cccaacacca aggagtggaa
     1021 gagcaaatgg ttctccaccc tgcccggtga aagcgggaag ttctacaacg acgtctggag
     1081 ccggcgctac ggcggcgacg tgcagggcat cagagaggcg ctgccctacc ttcgccagct
     1141 gggcatcaac gcgatctacc tcaatccaat ttttgaagcc gaggacatgc acaagtatga
     1201 cacctcggac taccgccacg tcgacgacaa ttttggcgtg aagggagacc tggccgagct
     1261 ggccagcgtc gaaaccgacg accccgcgac gtggcggtgg agcaagagcg accggatctt
     1321 cctcgacttc ctcgacgacg cccaccgcca gggcttcaag atcatcatcg acggcgtctt
     1381 caaccacgtc ggccgcaagc attgggcgtt tcaggacgtc atcaagaacg gaaagaactc
     1441 gaagttcgcc agctggttcg aaatcaccga ctggtccagt cccagcaaag acgccccgtt
     1501 ccactacaag gcctgggacg gcaacgacgg tgcgctgccg gattggaaaa aggacccgca
     1561 gttgggcatt gcgcacgggc cgcgcgagca catcctggcc atcaccaagc gctggctcgc
     1621 gcccgatggt gatccatcgc gcggcgtcga tggttggcgc ctcgacgccg ccaacgaagt
     1681 gccgcacccg ttctgggtcg agtggcgaaa gcttgtgaag tcgatcaagc ccgacgccta
     1741 tatcaccggg gaaatctggt cgtgggccca gccctggctc ggcggtaacg aattcgacgc
     1801 ggtgatgaac tatcgcttcg ccacgaccgg ccaggatttc ttcgtcaatc agcagaaggc
     1861 catcaactgc tcgcagttca acacgcgctg caacgagatc atcttcaact atccgtttca
     1921 ggtcgcgctg gtgaaccaga atttgttcga tagccacgac accgaccgct ttgcgtcgat
     1981 gttcgtgaac ccggatctgg cttacgacgc atcaaaccgc atccaggaca acggtccgca
     2041 atacaaggct gacaaaccca gcgacgagca gcgccagcgc atgctgcagg cggtggcgtg
     2101 gcagatgacg ttcgtcggcg cgccgatgat ctactacggc gacgaagccg gcatgtgggg
     2161 gccggacgat cccagcgatc gccagccgat gctgtggaag gacctcggta catacgacga
     2221 cccgcaagag caattcgacc agggcaagtt cgactggtac cagcgattga tcgcgatccg
     2281 cgccaaactg cccgcgctgc aaaccggctt cttccatccc gtcctgatcg acaacgcccg
     2341 cggcgaatat gtctacgccc gcgacctggg aagtcagcac gtgtacgtcg tcctgaatcg
     2401 caacagccgc gcccaaacgg tagaatttct cgcgggcgac cacgacgcga agcttatcga
     2461 ctggctcagt gccgcgcaga gcgacgtcaa cgcgccgaac gacggacggc caatcgtgga
     2521 attaaacggg aacgccaatt ccctcgacgc gcccggcgga cgcgcgagcg tgcaattgaa
     2581 accgtacggc gccgcgattc tgacggagaa agaataagaa agaccccgac gccggtgtaa
     2641 atccaagaac gtcatgacca tcccccgcgc atttcgcctg ctgctctcct tggccgcaat
     2701 tctcatcgtg gtctgggcat tcttcgacgt tatccaccgc ggcatccagc gcaacagcgc
     2761 ccagcacgac cgcccgatca cgctcaccgt cctgcactgg ggcgaccagg ctgaagacca
     2821 aatcatggag gcgctggatc gcgaattcga agatcagcac caaaacatcc gcatcgtccg
     2881 catcaacccc ggggccggcg attatgaaag caagctgaag acgatgctcg ccgcggggac
     2941 gccgccggat gtgttttacc tgcggccgga aatgctgcgc gatttcgcgt cgatgaagtt
     3001 gatcgctccg ctcgatcagc agttcgccgc cgagcctaag gagtggaagg acgatttcat
     3061 cccgatcatg ctcaacgcgt ggcgctacaa cgccgccacc gacaagatcg gcgatcacac
     3121 cgccacgctc tacggcctgc ccaaggactt caccaccgca gtgatgtacg tcaacctcga
     3181 cctgttccag aaggctggca tccccgtgcc ttacaagggt tggacctggg acgaatacga
     3241 agcggacatc aagaagatca ccgacctctc gggcacgccc gccgtcgaag gtcgcgcgat
     3301 ctacggcggg tccatcgaga tctggcacga caccgtccgc aatatcatct ggacctacgg
     3361 cggtgatttc ttcgggcccg gtgggttccg cgacgtgacg ctcgacgccc cgcccgcgca
     3421 agaagcgctg cagatgctcg cgcgcacgcg gcaggtcgct cacacggttt acaacccaac
     3481 cgtcgtcggt caggaaggcg gcgaagaatt tctgcgcggc ggcatcgggg tactcgggcc
     3541 actgggacga tggaaagtcc cgcgcatgaa ggaggtgacg gcgttcaagt gggactgcgt
     3601 cccgctgccg cacaagacgc aggaagcgtc gatggtctgg tacaccgcgt ggagcatgtc
     3661 cagcgccagc ccgcacccaa aggaatgcta tgagctgatc aagtatctga ccggcgcgaa
     3721 gggacaaatc ctggccgctc acctgggtct ggcggttccc tcgctcaagt ctgtcgcgta
     3781 ctcggatgat tttctcaatc cgccgggcat gccgaagcac aacagccagg cgtttctgga
     3841 tgcggtgaag gtggcgcgca tccagcaggt gccgcgcgaa ccggagtggg cgcgcatctg
     3901 cggtgaggaa ttgcaaaagg ccctgctgct cggcaccgaa aagccggcgc agatggcgca
     3961 ggtggtgaag cagcgctggc tgtcggagct ggattcaccg ctgcggcaac agcagtggca
     4021 gccgatgcgc tggaacatcg tcctgtcgat cacgatcgga ttgctcgtca cgctgttcgc
     4081 cgccttgtgg tggcgcgccc gcggcgaacg tcttggggcg ctcgatcgcg cgcaggaacg
     4141 tgccggctgg tcgttcatcg cgccgtgggt gatcggtttt ctcgcgctga cgctgggacc
     4201 aatgatcgcc tcgctgctgc tgagcttcac gcactggtcg gccatgtcgc ccatcagcga
     4261 agctgactcg gtgggcgcgg ctaactacaa gcaattgttc acgcacgacc agacattcct
     4321 gcattcgctg aacgtcacgg tgtactacgt gctgctggcg gtgccgatcg ggcaggccgc
     4381 cgcgctggcc gtcgccatgc tgatgaacct gcgccttagg agcatcaccg ccttccgcac
     4441 gatttacttc gtcccgtccg tcgtcggtgg cgctgcgctg gcggtgctgt ggcttcaact
     4501 gctcgacgac caatacggcc tggtcaacca catcatgcgg ccgatcctcg ggctggtgca
     4561 ccttcgcccg cccaactggt tcggcgcgga ctacgccacg tcgccgccca ccgacgacgc
     4621 cgcccgctgg gccattcccg cgttagtcat catgggcttg tggggcgtcg gcagcgggat
     4681 gatcatctac ctcgccgggc ttaaaggaat ccccgcgtcg ctttacgaag ccgcgacgat
     4741 cgacggcgcg gggccgaccg gaaaattttt caacgtcacg ctgccgatgc tctcgccgct
     4801 gatcttttac aacctggtga tgggcatcat cggcagcttc cagatcttca cgcaggcgaa
     4861 gatcatgacc aacggc
//
LOCUS       DATIMH010000672         1880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2182610, whole genome shotgun sequence.
ACCESSION   DATIMH010000672 DATIMH010000000
VERSION     DATIMH010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182610"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VLI90_11670"
     CDS             complement(<1..242)
                     /locus_tag="VLI90_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14912.1"
                     /translation="MSRITHQNAMRASARRSGAIWARHVLARGGATGAALPAASSGEI
                     ASIALVVIPRSPKDQQPFTTPVSRYGGGRLGYSARVR"
     gene            229..>1880
                     /locus_tag="VLI90_11675"
     CDS             229..>1880
                     /locus_tag="VLI90_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14913.1"
                     /translation="MILDIAIAALVAGLIVRRWVWDQTEPMRFVSDINNAFRQGTATL
                     RIGYLNRYDEDAGQHTFDQEFGFDYAPGRLAIATLWTRYVRTKLDGPSQDWRFVEDWS
                     QPFYARARSAVTPEGKPLTPYDLVRPLLIVNTTGEILAAIALFILVRRWTSGQRDSTP
                     GSEGTFDDPDSSAARQEAPGSSEYLRTRRGAGMKPTILGLIAALFFWFNIAVIWNAHA
                     WPQWDSWVLPFLLWALVAASFDCWFVAGALIATGAMFKGQILFSAPLFLLWPLWRGWP
                     LAIARWLIGAATAVAACTGVWLLRSHEEFNPDAIRWVITLAIAFALLVIALRPRWRWY
                     VAMPIAAVACGIILWQFPILGARWILAASGATFLVAAIVTWASWRAIAYAAAAWVSTS
                     VLLCAVLFGGSMAWYQIGIAYGARHYMQLTRGPANNLASILEENYQWRAPMETAMTLQ
                     PGRVAGWIAPMVSSDQTTPYTPGQPLDIPLRSLLGAIYAVFVVLCSIGAAIQSKRGSP
                     RFLIAVTAPWIVMFAVLPQMHERYLMWGAALSAMTVAISPGLA"
BASE COUNT          280 a          644 c          600 g          356 t
ORIGIN      
        1 cgcacgcgag cgctgtaccc gaggcgcccg ccaccgtatc tagataccgg cgttgtaaag
       61 ggttgttgat cttttggcga acgcggaatg acgacgagcg cgatcgaggc aatttcaccg
      121 gaagaagcgg cgggaagcgc ggctcccgtc gcgccgccgc gcgcgagaac atggcgcgcc
      181 caaattgcgc cgctgcgccg cgcgctggcg cgcatcgcgt tctggtgcgt gattctcgac
      241 atcgccatcg ccgcgctggt cgccgggtta atcgttcgcc ggtgggtctg ggatcagacc
      301 gagccgatgc ggttcgtcag cgacatcaac aacgcattcc gccagggcac ggccacgctg
      361 cgcatcgggt atctcaaccg ctacgacgag gacgccggcc agcacacgtt cgaccaggaa
      421 ttcggattcg actacgcccc cggtcggctg gcgatcgcga cgctctggac gcgatacgtt
      481 cgaacgaaat tggacggccc gtcgcaggac tggcggttcg tggaggattg gtcgcagccg
      541 ttctacgccc gcgcccgatc cgcggtgacg ccggagggta agccgctcac gccatacgat
      601 ttggtccgcc cgctgctgat cgtcaacacg accggcgaga tcctcgccgc catcgcactc
      661 ttcatcctcg tccgccgatg gacgagtgga cagcgcgact ccacgccggg ttcggagggt
      721 actttcgacg acccggattc ttctgcggcg cgccaagaag cgcccgggtc gtcggagtac
      781 ctccgaaccc ggcgtggggc cggaatgaaa cccaccatcc tcggcctcat cgccgcgctc
      841 ttcttctggt tcaacatcgc cgtcatctgg aacgcccacg cgtggccgca gtgggacagc
      901 tgggtgttgc cgttcctgct atgggccctg gttgccgcga gcttcgactg ctggttcgtc
      961 gccggcgcgc tgatcgccac cggggcgatg ttcaaagggc agatcctctt cagcgccccg
     1021 ctgtttttac tctggccgct ctggcgcggg tggccgctgg cgatcgcgcg ctggctcatc
     1081 ggcgccgcca ccgccgtcgc cgcgtgcacc ggtgtctggc tgttgcgatc gcacgaggag
     1141 ttcaatcccg atgcaattcg atgggtgatc acgctcgcca tcgcgttcgc gctgctggtc
     1201 atcgcgctgc ggccgcgctg gcggtggtat gtcgcgatgc ccatcgccgc cgtcgcctgc
     1261 ggcatcattc tctggcaatt cccaatcctt ggcgctcgat ggattctcgc tgcctccggc
     1321 gcaacgtttc tcgtcgcggc catcgtcacc tgggcatcgt ggcgcgcgat cgcctacgcc
     1381 gccgcggcgt gggtttcgac atcggtcctg ctctgcgccg tcctcttcgg tggcagcatg
     1441 gcgtggtatc agatcggcat cgcctacggc gcgcggcatt acatgcagct gacccgcggg
     1501 cctgccaata atctcgcaag catcctggaa gagaattacc aatggcgagc gccgatggag
     1561 acggcgatga ccctccagcc cggccgggtt gccggctgga ttgctccgat ggtctcgtcc
     1621 gatcagacga cgccgtacac gcccggacaa ccgctcgaca tcccgctgcg atcgctgctc
     1681 ggtgccatct acgccgtctt cgtcgttctc tgttcgatcg gcgccgccat ccaaagcaaa
     1741 cgcggcagcc cgcggttcct catcgcggtc accgcgccgt ggatcgtgat gtttgcggtg
     1801 ctcccgcaga tgcacgagcg atacctgatg tggggggcgg ccctgagcgc gatgaccgtc
     1861 gccatcagcc caggcctggc
//
LOCUS       DATIMH010000673         1873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4204047, whole genome shotgun sequence.
ACCESSION   DATIMH010000673 DATIMH010000000
VERSION     DATIMH010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4204047"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1586
                     /locus_tag="VLI90_11680"
     CDS             105..1586
                     /locus_tag="VLI90_11680"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HSV14914.1"
                     /translation="MNAMNAISMNAVTLFYLVASVCFIQALKGLSSPSSARTGNAFGM
                     AGMAIAVLTTVALMLKLQDQLQSAMQDAQHGAGMGFVRVLLGMAVGGAIGAVAARRVE
                     MTKMPELVAAMHSLIGLAAVCIAIAAVAEPWAFNITARGQLLPFGNRLELFIGTFVGA
                     VTFSGSVIAFGKLAGKYKFRLFQGAPVRYPAQHPINLAVLVAIVVLGLAFCSSLSWPA
                     FVAMAALAFLLGVLIIIPIGGADMPVVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLV
                     GSSGAILSYIMCRAMNRSFFNVLLGGFGNEGGAAAVSGLTEQRPVKSGSADDAAFMLG
                     NAESVVIVPGYGLAVARAQHALKELTDKLVEKGIDVRYAIHPVAGRMPGHMNVLLAEA
                     EVPYELVHEMEDINSEFGQADVVLVLGANDVVNPAAKTDPNSAIAGMPILEAYKARTV
                     IVNKRSMAAGYAGLDNELFYMDKTMMVFGDAKKVVEEMMKAVD"
BASE COUNT          316 a          523 c          659 g          375 t
ORIGIN      
        1 ttcctcgtca cgctgcgcat gctggaaatg ttccggaaaa aggagcggcc gccccgtgtg
       61 gatgcggagg cgccggccgg cgcgaaggcg cagcgggagg tcgcatgaac gccatgaacg
      121 cgatcagcat gaatgccgtc acgctgttct atctggttgc gtccgtgtgc ttcatccagg
      181 cactgaaggg cttgtcgtcg ccgtcgtcgg cgcgcaccgg caacgcgttc ggcatggcgg
      241 gcatggccat cgcggtgctg acgacggtgg cgctgatgct gaaattgcag gaccagctgc
      301 agagcgccat gcaggacgcg cagcatggcg ctggcatggg cttcgtgcgc gtcctgctcg
      361 gcatggcggt gggcggcgcg atcggcgccg tggccgcgcg ccgcgtcgaa atgaccaaga
      421 tgcccgaact ggtggcggcc atgcactcgc tgatcggcct cgcggccgtg tgcatcgcca
      481 ttgccgccgt cgccgagccg tgggcgttca acatcaccgc gcgcggccag ctgctcccgt
      541 tcggcaaccg cctggaactg ttcatcggca ccttcgtcgg cgccgtgacg ttttccggtt
      601 ccgtgatcgc cttcggcaag ctggccggca agtacaagtt ccggctgttc cagggcgcgc
      661 ccgtgcgcta cccggcccag caccccatca acctggcggt gctggtcgcc atcgtcgtgc
      721 tggggctggc gttctgctcc tcgctgtcct ggcccgcgtt cgtcgcgatg gccgcgctgg
      781 ccttcctgct gggcgtcctg atcatcatcc ccatcggcgg ggccgacatg ccggtggtgg
      841 tgtcgatgct gaactcgtac tcgggctggg cggcggccgg catcggcttt tcgctgaaca
      901 atccgatgct gatcatcgcg gggtcgctgg tgggttcgtc gggtgcgatt ctgtcgtaca
      961 ttatgtgccg ggcgatgaat cggtcgttct tcaacgtcct gcttggcggg tttggtaacg
     1021 aaggtggtgc agctgcggtg agcggcttga cggagcagcg gccggttaag tccggttcgg
     1081 ctgatgacgc tgcgttcatg cttggtaacg cggagagcgt ggtgatcgtg ccgggctatg
     1141 ggctggcggt cgcgcgtgct cagcacgcgc tgaaggagct gaccgacaag ctggtcgaga
     1201 aaggcatcga cgtgcgttac gcgattcacc cggtcgccgg gcggatgccc gggcacatga
     1261 acgtgctgct ggcagaagcg gaagtaccgt atgagttggt ccacgagatg gaggacatca
     1321 acagcgagtt cggccaggcc gacgtggtgc tcgtgctcgg cgccaacgac gtggtgaatc
     1381 cggccgcgaa aactgatcca aattcagcaa tcgcgggaat gccgattctg gaagcgtata
     1441 aggcacggac ggtcatcgtg aacaagcggt cgatggcggc gggttacgcc ggtctcgaca
     1501 acgagctgtt ttatatggat aagacgatga tggtttttgg tgatgcgaag aaggtggtgg
     1561 aggagatgat gaaggcggtc gattgattga gcacagaccg gacatcgtgc cgggtggccc
     1621 gcgcggtcct gcttggcctg atccgaagag aatggtggcg gcgaccacgg agttttgctt
     1681 caataggcat aacgcattac cctggagact gcaaccgcaa cggccctgcg taagtctgct
     1741 gttcagatcg atgggctcgc atcgcacgat taatgagaat cgatcgttaa ttgatgacgg
     1801 tgtgacggag ggcagaagag atgaatgtga ctgacgtagt gatcgtatcg gccgcgcgta
     1861 cggcggttgg caa
//
LOCUS       DATIMH010000674         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4204120, whole genome shotgun sequence.
ACCESSION   DATIMH010000674 DATIMH010000000
VERSION     DATIMH010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4204120"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VLI90_11685"
     CDS             complement(<1..904)
                     /locus_tag="VLI90_11685"
                     /inference="COORDINATES: protein motif:HMM:NF015350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STELLO glycosyltransferase family protein"
                     /protein_id="HSV14915.1"
                     /translation="MEPGLTCVITTIQEPTASVLGIMRALSRRSPAGKLIAIGDQKGP
                     RQFDLPNADFYSLDRQRELPFSLAGLLPTKHYARKNLGYLIAMKEGAPVIYETDDDNA
                     PLDAWKPLPRTAQAIRIGGQRWFNVYRLFSDELIWPRGMPLDRVRDAGTYSYDASTKA
                     TAIDSPIHQGLADLAPDVDAVWRLILDHDFTFRPGPSILLDPGTWCPFNSQNTWWWPA
                     AYQLMYLPSYCSFRMTDIWRSFIAQRCLWETGAGVVFHQADVEQQRNAHNLMRDFMDE
                     VPGYEGNQRLIDVLRETKLQGGPAN"
     gene            complement(889..1656)
                     /locus_tag="VLI90_11690"
     CDS             complement(889..1656)
                     /locus_tag="VLI90_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV14916.1"
                     /translation="MTQSSNPSHSSTPRLGIVVPLANEEATIDEFLRRVTAQLGADDR
                     LFCVLDNVCKDQTRARVDAKAAEDPRVVCVWAPQNRCVVDAYFSGYRAALDANCRWIL
                     EMDGGLSHAPEKIPEFVQAMETGVDFAPGSRFCKGGKYSGRWTRWALSKGGTVLATLV
                     LGTRMSDMTSGFECFTHRALRHVVARGVKSRAHFFQTEIRYMLQDWRWQEVPISYSSP
                     SETVGSSTVGEALKNLWHLRKESRLSVPTGENAWNPA"
     gene            1879..>2613
                     /locus_tag="VLI90_11695"
     CDS             1879..>2613
                     /locus_tag="VLI90_11695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV14917.1"
                     /translation="MSDHRRNQAAGERASRRYHDRVARQYDSIYDDPYWVFHDELTWR
                     RVKPWLPRNATAACADLGCGTGKWGLKLLKSGFATTFVDNAAAMIEQVRPKLEALGPK
                     GRKGTLIVADIVEMPAVPSDAFELVLAMGDPLSICSDAKRACREMARITRTGGVVIAT
                     ADNKLAAIDHFVERGSIDALEEFVRGGRTHWLTADERERFELTTFTPASLRRLFESNG
                     FEAIEVTGKTIIPVRQNKRLLEDPSAV"
BASE COUNT          473 a          807 c          844 g          489 t
ORIGIN      
        1 cgttggccgg gccgccttgc agcttcgttt cgcgcagtac gtcgatcagc cgctggttgc
       61 cctcgtaacc cggcacttca tccatgaagt cgcgcatcaa attgtgggcg ttgcgttgtt
      121 gctcgacgtc ggcttgatgg aagacgacgc cggcgccggt ctcccacagg cagcgttggg
      181 cgatgaagct tcgccagatg tcggtcatgc ggaacgagca gtagcttggc aggtacatca
      241 gttgatacgc cgcgggccac caccaggtgt tctggctgtt gaacgggcac cacgtgccgg
      301 gatcgagcag gatgctcggc ccggggcgga acgtgaaatc gtgatcgaga atcaaccgcc
      361 aaaccgcgtc cacatcgggc gcgagatcgg ccaatccttg atggatcggc gaatcgatcg
      421 ccgtcgcctt ggtcgatgcg tcatacgaat acgttcccgc gtcgcgcacg cgatcgagcg
      481 gcatgccgcg cggccagatc agctcatcgc tgaacagccg atagacgttg aaccaccgct
      541 gcccgccgat gcgaattgct tgcgcggtcc gcggcagcgg cttccacgcg tccagcgggg
      601 cgttgtcatc gtcggtctcg tagatgacag gcgcgccttc cttcatggcg atgagatagc
      661 ccagattttt gcgcgcgtag tgcttggtcg gcagcagccc cgccagcgag aacggcaact
      721 cgcgctgccg gtccagcgaa tagaaatcag cgttgggaag atcgaactgc cgcgggcctt
      781 tttgatcgcc gatggcgatc agtttccccg cgggggatcg gcgcgacagg gcgcgcatga
      841 tgcccaggac gctggccgtg ggttcctgga tcgtggtgat cacgcacgtc aggccgggtt
      901 ccaagcattt tctccggttg gaacgctcag gcgtgattcc ttgcgcaggt gccacaggtt
      961 cttcagcgcc tcgccaaccg tgctgctgcc gacggtttcg ctggggctgc tgtagctgat
     1021 cggcacttcc tgccagcgcc agtcctgcag catgtagcgg atttcggttt ggaaaaagtg
     1081 cgcccggctc ttcacaccgc gggcgacgac gtgccgcagg gcgcggtgcg tgaagcactc
     1141 gaacccgctg gtcatgtcgc tcatgcgcgt gccgaggacc agggtggcta aaaccgttcc
     1201 acctttgctc agcgcccagc gggtccatcg cccgctgtac tttccgcctt tgcagaaccg
     1261 gctgccgggg gcgaaatcaa cgccggtctc catcgcctgc acgaattccg gaatcttctc
     1321 cggcgcgtgg ctcaagccgc catccatctc cagaatccaa cggcagttcg catcgagcgc
     1381 ggcccgatat ccgctgaagt acgcatcgac gacgcagcgg ttctgcggcg cccacacgca
     1441 aaccacgcgt ggatcttcgg cggccttggc atcgacacga gcgcgggttt gatccttgca
     1501 gacattgtcc agcacgcaaa aaaggcggtc gtcagcgccg agctgagccg tcacacggcg
     1561 caggaactcg tcgatcgtcg cctcttcgtt cgccagcggc accacaatgc ccaggcgcgg
     1621 cgtggaactg tgggatgggt tggatgattg ggtcattggt cctgccgttc cctaaggagt
     1681 gggctaataa ccgatacatg tctgcttatc aagcaacgcc accgctggcg caaggctgac
     1741 gccggttcaa aagccggtgc gggctgatgc agttttacat cggatgcgcg tggaaggttc
     1801 gcgtaaatcc gttggaccgt ctatcgcagc cgcgggaagc attcccaaca tccgcgtgga
     1861 cctataccat cccacgcgat gagcgatcac cggcggaacc aggcggcagg cgagcgggcg
     1921 tcgcggcgat atcacgaccg tgttgcccgg cagtacgact cgatctatga cgatccttat
     1981 tgggtgtttc acgatgagtt gacctggcgg cgggtgaagc cgtggctgcc gcggaacgcg
     2041 acggcggcgt gcgccgacct gggctgcggc accggaaagt gggggctcaa gctgctgaag
     2101 agcggcttcg ccacgacgtt cgtcgataac gccgccgcga tgatcgagca ggtccggccg
     2161 aagcttgaag cgctgggacc caagggtcgc aagggcacgt tgatcgtcgc cgacatcgtc
     2221 gagatgcccg cggtgccgag tgatgcgttc gagctggtcc tggcgatggg cgatccgctg
     2281 tcgatctgtt ccgatgcaaa acgcgcctgc cgcgagatgg cccgcatcac gcggaccggc
     2341 ggcgtcgtca tcgcgaccgc cgacaataag ttggcggcaa tcgatcattt cgtcgaacgc
     2401 ggctcgatcg acgcgctcga agagttcgtc cgcggcgggc gcacgcactg gttgaccgcg
     2461 gacgaacgcg agcggttcga actgaccacg ttcacccccg cctcattgcg gcggttgttc
     2521 gaatccaacg gattcgaggc gatcgaagtc accggcaaaa cgatcattcc ggtccggcaa
     2581 aataagaggc tgctggaaga tccgagcgcg gtc
//
LOCUS       DATIMH010000675         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2182819, whole genome shotgun sequence.
ACCESSION   DATIMH010000675 DATIMH010000000
VERSION     DATIMH010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182819"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VLI90_11700"
     CDS             <1..1222
                     /locus_tag="VLI90_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14918.1"
                     /translation="RLGTPHLPEAGNYFVTYDDYVAQHPGTMVEDDPTDPNGPVVWPN
                     KVSDLTRQWLKSNEKPLALLAEATKRPRYFIPFYGGFRTQTLIEVLIPHVGLLRQTRR
                     PLLTRAMLRLNDGDYAGFHDDVMTVHWLARLMAQSSTLVERMVAKDMDIAACRIEGAA
                     AAGGKLSVEQCKSLAAESIALGDLQPITDAWNMGERLMGLDVLQMLARSNPVHAGTYL
                     NAAIGGSSQNAVARIEPPFAFLFIPISYEQNMIALNHFHDSAMAAIELRSYPQRAAMM
                     RLWAKQLNESTRHGPVMILTTPDWADALLLPQLDRTIANAEIARVQRRLTQVALALAA
                     FKAEHAGTYPAALDELSPQYLPAVPSDLFLDKPLIYSRTNMGYALRSVGPNMTDDGGA
                     ATGKGRDDIVADVP"
     gene            1389..1892
                     /locus_tag="VLI90_11705"
     CDS             1389..1892
                     /locus_tag="VLI90_11705"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSV14919.1"
                     /translation="MQDTTQQSQNIEKLASLIKGVRIAMLTTTDGDGSLHSRPMATQS
                     ADFDGELWFLTGQRSHKVQEINRDHHVSLSYADPSDNRYVSISGTARLVRDRKRAEDL
                     WNPMYKAWFPKGLDDPELCVLRVTPERAEYWDSPSSAVVHMVGFVKAALTGKPADAGE
                     HGEMRLV"
     gene            complement(2005..3387)
                     /locus_tag="VLI90_11710"
     CDS             complement(2005..3387)
                     /locus_tag="VLI90_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HSV14920.1"
                     /translation="MSILALTPVAFRLGWRPSSNDNASADHPTSAASMVNIRYIAWGN
                     PQQLECERAIIQEFNSKCAAEGKLIHVNLEMPPAGGYPQRLLMMFASGTAPDVCRVDH
                     YNFAAWTAKGYIRDLTDMAADDPTFHIQDFHPAALQEDYYHGRLCGLTVLFGGLVCYY
                     NQDLFRRAGLHDPYDLWREGKWTWQAFDDAADRLTIRVADGQTPVYGCSFYNLPNCPA
                     PAVWALWIWREGGTLLSPDHAHSMLDSPEAIRGITRMRDMVYTRRVSPSPAQVASNLY
                     SFEAGNVGMYIDFAGLAPRYRELIQDFQWDIVPTPSNPNDPYTMTKGNQVVISAKCPH
                     PKEAWEWIKFLTSRRAELFLCGDAYRRCIPTRVALLHDPEYLEAKQRPYHADVFADLL
                     DHPRALPIDESWFTWTTTATRFMERLYIDGNTDVERTMTAAASAIDQELAREHERYRR
                     YSETGLAHAD"
     gene            4245..>4714
                     /locus_tag="VLI90_11715"
     CDS             4245..>4714
                     /locus_tag="VLI90_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14921.1"
                     /translation="MSRINTNVSSLIAQRTLATNNANLQTSLQRLSTGLKINSGADDP
                     AGLIASENLKSEQAGITQAIGNAQRASNIIGTAEGGLNEVSSLLTQVQSLVSQSANSG
                     GLSTDEISANQLQVDSILSTVNRIAGSTQFQGKKLLNGDYAYTTSTAQASAFASL"
BASE COUNT          934 a         1446 c         1513 g          821 t
ORIGIN      
        1 tcggctcggc acgccgcatt tgccggaggc ggggaattat ttcgtgacgt atgacgatta
       61 cgttgcgcag cacccgggga cgatggtgga ggacgatcca accgatccga atgggccggt
      121 cgtctggccg aacaaggtgt cggatttaac tcggcagtgg ctgaagtcga acgagaaacc
      181 gttggcgctg ctggcggagg cgacgaagcg gccgcgctac ttcatcccgt tctatggcgg
      241 ctttcgcacc cagacgctga tcgaagtgct catcccccac gtgggcctgc tgcggcagac
      301 gcggcggcct ttgctgacgc gcgcgatgtt gcggttgaac gacggcgact acgcgggatt
      361 tcacgacgac gtgatgacgg tgcattggct ggcgcggttg atggcgcagt cgtcaacgct
      421 cgtcgagcgc atggtggcca aagacatgga catcgccgcc tgccgcatcg agggcgccgc
      481 ggcggccggc ggaaagctct cagtggaaca atgcaaatcg ttggcagcgg aatccatcgc
      541 gctgggtgac ctgcaaccga tcaccgacgc ctggaacatg ggcgagcgcc tcatggggct
      601 cgacgtactt caaatgctgg cccgcagcaa cccggtgcac gcgggcacct atctcaacgc
      661 cgccatcggc ggcagcagcc aaaacgcggt ggcgcgaatc gaaccgccgt tcgcgttcct
      721 gttcattccc atctcttacg agcagaacat gatcgcgctc aatcatttcc acgacagcgc
      781 gatggccgcg atcgagttgc ggagttaccc gcagcgggcg gcgatgatgc ggctgtgggc
      841 gaagcaactg aacgaatcga cccgccacgg gccggtcatg attctcacca cgcccgactg
      901 ggccgacgcc cttctgctgc cgcagttgga tcgcacgatc gcgaacgcgg aaatcgcgcg
      961 ggtgcagcgc cggttgacac aggtcgcgct ggcgctggcg gcattcaaag cggagcacgc
     1021 cgggacgtat ccagcggcgt tggacgagct atcgccgcaa tatctgccgg cggtgccgag
     1081 tgatttgttc ttagacaagc cgctgattta ctcgcggacg aatatggggt acgcgctgcg
     1141 cagcgttggt cccaacatga cggacgatgg cggcgccgcc actggcaaag ggcgggatga
     1201 tatcgttgcg gatgttccat gaagcgccgc cgcttgcggc ttcgcagacc acgccgtttg
     1261 gattgcgaag ccgcaagcgg cgggactgct gcgactactg aaattcaccc gcacaattcg
     1321 cgcgttccca cgccgcgtcg ttccgactta gcatcgggcg cgtttctttt tccaacggag
     1381 ccccgcccat gcaggacacc acgcagcagt cgcagaatat cgaaaagctt gcgagcctca
     1441 tcaagggcgt gcgcatcgcc atgctcacga cgaccgacgg cgacggctcg ctgcacagcc
     1501 ggccgatggc gacgcagtcg gcggatttcg acggggaact gtggttcctc accgggcaac
     1561 gcagccacaa ggtgcaggaa atcaaccgcg accatcacgt gagcctcagc tacgccgacc
     1621 ccagtgacaa ccgctacgta tcgatcagcg gcaccgcccg gctggtgagg gaccgcaaac
     1681 gggcagagga cctgtggaac ccgatgtaca aggcgtggtt ccccaagggg ctggatgatc
     1741 cggaattgtg cgtgctgcgg gtgacgccgg agcgggcgga gtattgggat tcgccgtcga
     1801 gcgcggtggt gcacatggtg ggtttcgtga aagccgcgct gacgggaaaa ccggccgatg
     1861 cgggggagca tggcgagatg cgcttggtgt gatcattaaa tgcggcgcgc tgcggtatcc
     1921 ggggcgccgc atcagacccg taaaagcgcc gcagcgcgcc gcacttcact acggctccga
     1981 tcgacgtcgc atgcgtttaa ccgctcaatc cgcgtgggcg aggccggttt cggaatatcg
     2041 gcgatagcgc tcgtgctcgc gggccagctc ttggtcaatg gcgctcgcgg cggcggtcat
     2101 ggttcgctcc acgtccgtgt tgccgtcgat gtagaggcgt tccatgaagc gcgtcgccgt
     2161 cgtcgtccac gtgaaccagg actcatcgat cggcagcgcc cgcggatggt ccagcaggtc
     2221 ggcgaagaca tcggcgtgat agggccgctg cttcgcttcg agatattccg gatcgtgcag
     2281 cagcgccacg cgcgtcggaa tgcaccggcg gtacgcgtcg ccgcacagga ataattcggc
     2341 gcggcggctc gtgagaaact tgatccactc ccacgcttcc ttggggtgcg ggcacttggc
     2401 gctgatcacc acctggttgc ccttggtcat cgtgtacgga tcgttcgggt tcgacggcgt
     2461 cggcacgatg tcccactgaa aatcctggat caactcgcgg taacgcggcg ccagcccggc
     2521 gaagtcgatg tacatgccga cgttgccggc ctcaaacgaa tacaggttcg acgccacctg
     2581 cgccggggag ggcgagacgc gccgggtgta gaccatgtcg cgcatgcgcg tgatcccgcg
     2641 gatcgcttcg ggggaatcga gcattgagtg cgcgtggtcc ggcgacagta acgtgccgcc
     2701 ctcgcgccat atccaaagcg cccacaccgc gggggcgggg cagttgggca ggttgtaaaa
     2761 gctacagccg tacacgggcg tctgcccgtc ggcgacgcgg atggtcaacc gatccgcggc
     2821 gtcgtcgaag gcttgccaag tccatttgcc ttcgcgccac agatcatatg gatcgtgcaa
     2881 acccgcgcgg cggaacaggt cctggttgta gtaacacacc agccccccga acagcacggt
     2941 cagcccgcac agccggccgt ggtaataatc ttcctgcaac gcggcggggt ggaagtcctg
     3001 gatgtggaac gtcgggtcgt cggcggccat gtcggtcagg tcgcggatgt accccttggc
     3061 ggtccacgcg gcgaagttgt agtggtccac ccggcacaca tcgggcgccg tgccgctggc
     3121 gaacatcatc agcagccgtt gcggatatcc cccggcgggg ggcatttcca ggttgacgtg
     3181 aatgagcttt ccctcggcgg cgcacttgct gttgaactcc tggatgatcg cgcgctcgca
     3241 ctccaactgc tgcgggttgc cccaggcgat gtagcggatg ttcaccatcg acgccgcgct
     3301 cgtcgggtga tcggcgctgg cgttgtcgtt ggacgaaggg cgccaaccta accgaaaggc
     3361 gacgggagtc agcgcgagaa tggacacgat gacggcggcg atacgccgcc aaggcgttcc
     3421 gtcttcatca acggaatcgc gtcgagtcgg tggattgggc agaggggatt gagacataca
     3481 tcgcacacgc agacacgcgg cgttacgagc taactcacca aacgtcagcc gagagacatg
     3541 acacgcgagc gggcccttcg cgaacgccag aagcaaccaa ccgtcgcgag cgtattttgc
     3601 catgagttgt gccggaagtg caaacatcgt ggcgctgaat ttgatcgtta tcctccagcg
     3661 catgcgtacg ccggagtcat atgcctctcg gcaagatcct tcgactgcgc acgagtacgt
     3721 gcttcgctcc ggatggcatt gatcatgtcc cggcccgcat aagtctgata acaacgcggc
     3781 acgtcaaacc agcaccgtca cttcgtcggg atcacaacgt ttcgacgccc ttcgccccat
     3841 aacccgctcg gtatttcctg catggtccgc gtctaaagtc cccctattcc cggcgacgat
     3901 gcagaccgca gagattgaaa aaccatcgat ggcggcacgc gcttcaaaac gctgccgcgg
     3961 catcgggtgg ttgggacttg taagaaagga gcgactgcat gatccaacat cggttggacc
     4021 gccgtggcca ggactgaaac tggcgccgcg cggtatcaac cgcaacaggc tgtagtgctg
     4081 gacgcggtga acccctgacc gcaccggcga cgaaggagca tgcaagtacg cacgcacgtt
     4141 gtaagacgtt ccttcccggc ctttcaaggg aatgaagggc gcgacgttga cctggaccgc
     4201 agatccgggc cgtcgcgcaa aacaccagtc aaggaaagac aatcatgagc agaattaaca
     4261 ccaacgtttc gtcgctgatc gcacagcgaa ctttggcgac gaacaacgcc aacctgcaga
     4321 cctcgctgca gcgcttgagc accggtttga agatcaacag cggcgcggat gacccggctg
     4381 gtttgatcgc cagcgagaac ctgaagtccg agcaggccgg catcacgcag gccatcggca
     4441 acgcccagcg cgccagcaac atcatcggca ccgctgaagg tggtttgaac gaagtcagct
     4501 cgctgttgac gcaggtccag agcctggtca gccaaagcgc caacagcggt ggtctgagca
     4561 ccgacgaaat cagcgctaac cagcttcagg ttgactcgat cctgagcacg gttaaccgca
     4621 tcgccggttc gacgcagttc cagggtaaga agctgttgaa cggcgactac gcctacacga
     4681 ccagcacggc ccaggcgagc gcgttcgcca gcct
//
LOCUS       DATIMH010000676         7258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4204352, whole genome shotgun sequence.
ACCESSION   DATIMH010000676 DATIMH010000000
VERSION     DATIMH010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4204352"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /gene="fxsT"
                     /locus_tag="VLI90_11720"
     CDS             <1..1773
                     /gene="fxsT"
                     /locus_tag="VLI90_11720"
                     /inference="COORDINATES: protein motif:HMM:NF040586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxSxx-COOH system tetratricopeptide repeat
                     protein"
                     /protein_id="HSV14922.1"
                     /translation="RADYSVVWWIRADSAASIAHSFLSLSARLGKQLSQDAPATLIID
                     SLKQLLARRDWLMIFDNASGADAIRPFIIGEGNGHVLITSRNPNWAGVAVSQPLKVMT
                     RDESIEFLMRRTRRSETRDAADKLANALGDLPLALEQAAATIEQTGVTFADYLRRFET
                     EWADLLQEGVKPVDYPHTVAMTWGLAFNAVETASAAAAELLNLCAFLCPDRITRKMLW
                     EGEEHLPPRLRKFTDPLRLEDAISPLRNYSLVDADDRNISMHRLVATITRDRLDEERQ
                     IVWCNAAIEFLSGMFQFDSANVDSWAKCGELLPHVMDASSHADRLKVAPDITSELLND
                     AGRYLLKRGQYADAKAALDKALAICSRRYGTGHPKISSITNNLGRVHQNLGDDAAAIQ
                     CFEHALSIDEATYGHSHPHVAEVINNYGICLQKKGDSELARQQFEWAAQVYETHYGPD
                     HAKLAHILNNLGYVLMASGNIESAHPHITRALEIAEKTVGPSHPTTARILYNLATVYR
                     VTNQLPAAKEHLERALRIDETVLGPNHPDVANDCIAMSELLNKMGQAAMANAYYERAT
                     QIRANSGSPPPQRQGAPAAVSMVS"
     gene            2088..2972
                     /locus_tag="VLI90_11725"
     CDS             2088..2972
                     /locus_tag="VLI90_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14923.1"
                     /translation="MILRSLIASLTITLLASCVNSGAGAGAVVNPSFPISEPAARDVI
                     RNIARQHRPLPRPLVIIGGFRDPGIAAPILKHEFAKWTGSGDRIVTVSLAMTFSFDQC
                     RQRIIDAVDQAFPSDDPRQTIEVDVIGFSMGGLAARYAASDVPPEQGEPATRRLRIAR
                     LFTISSPLRGAAVAAELPLTLHPLQPPMRPGSRFLRAIDAAPVRPDELYQIFSYVRLG
                     DPYIREANSAINRETAWWVSTPPLSPPHEMAFQDGRILADILCRLLGEPPLTSDPPTP
                     LPNPQQAAAAAAHVPNLE"
     gene            2998..3471
                     /gene="folK"
                     /locus_tag="VLI90_11730"
     CDS             2998..3471
                     /gene="folK"
                     /locus_tag="VLI90_11730"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HSV14924.1"
                     /translation="MQRTASITAYIGLGSNLGDRAGNLNAAAERLGQPDHTRVVATSS
                     FLENPAVGGPPDSPPFLNAAVKIETTLGSHALLHRLLEIEREMGRDRRAKWEPRTIDL
                     DLLLYGDQIISSQELIVPHPLMHERRFVLQPLAEIAPDVVHPMLQMTMAGLLATV"
     gene            complement(3649..3885)
                     /locus_tag="VLI90_11735"
     CDS             complement(3649..3885)
                     /locus_tag="VLI90_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HSV14925.1"
                     /translation="MTEEKTSNVVDADLLQILRCPLTRSRLRQEGDWLVAEVGGLSYP
                     VREGIPVMLIEEAKLPAGVASLEDFKREFGVGAA"
     gene            complement(3898..4809)
                     /locus_tag="VLI90_11740"
     CDS             complement(3898..4809)
                     /locus_tag="VLI90_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14926.1"
                     /translation="MTDPTGIQNELRRYAALIEPMTAPPPGFTAEAEDARSIQVERAI
                     VFPSTLNGGWSAERATSVNRLVELSRLANLQDIAVVDRSGHHRPVYRGLLIYAWLQAY
                     RLAYETMPRDVFGQWEEGLRPWCDLLESLIGDVEWFDGPIPAGRGARLAEAAWMALAL
                     HIAGKAFVRDAWTDLAGDVFGKITRAQQSSGSFLATSARDNPETHWYHELVILHAAAS
                     YAVQAEDRTVAAAVAHNGEWHLRETQPDHATTQPWGVFAFAWNAATRSLADQVLHAAA
                     TQAPGAGIDGVSLVLLADALYCLRLFL"
     gene            4905..5489
                     /locus_tag="VLI90_11745"
     CDS             4905..5489
                     /locus_tag="VLI90_11745"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HSV14927.1"
                     /translation="MTTITAPASSLPSDHADLSAGRGWLKRVPLLTGVLAALAGFLTV
                     RGANLSNQAIYHSNQGVLHQARASDAWAEYQANSIKARIVETTLLTAIDTASKDALNT
                     QAKELRVRQPTIRATAEDQEQQRDAELTGGQRRLAEKDLLDYAGMAAQLGIALASVAA
                     LTRRRDAFAVGVVCGALGVAITAYAIVVNHFVHA"
     gene            complement(5690..6331)
                     /locus_tag="VLI90_11750"
     CDS             complement(5690..6331)
                     /locus_tag="VLI90_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HSV14928.1"
                     /translation="MPGKFHHESLYRGDALLEHLATSHITLCGAGAVGSNLADALARQ
                     GVAHLRVIDHDRVEEHNVSTQIYGEGDVGVWKVEALRNRLFRTVGVEIGAMRKELSAA
                     NARQLLKDSELIVDAFDNAASRQLVQDHARATATPCLHVGLFEDYGEVVWDERYRVPR
                     DVGNDVCDYPLARNLVLLATLVAAEAVVRFIMNGERVSWTATLRDLAVRPMDD"
     gene            complement(6749..6823)
                     /locus_tag="VLI90_11755"
     tRNA            complement(6749..6823)
                     /locus_tag="VLI90_11755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6786..6788),aa:Arg,seq:ccg)
     gene            complement(6927..7000)
                     /locus_tag="VLI90_11760"
     tRNA            complement(6927..7000)
                     /locus_tag="VLI90_11760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6964..6966),aa:Leu,seq:cag)
     gene            complement(7021..7092)
                     /locus_tag="VLI90_11765"
     tRNA            complement(7021..7092)
                     /locus_tag="VLI90_11765"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7058..7060),aa:Lys,seq:ttt)
     gene            complement(7099..7173)
                     /locus_tag="VLI90_11770"
     tRNA            complement(7099..7173)
                     /locus_tag="VLI90_11770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7136..7138),aa:Arg,seq:tct)
BASE COUNT         1346 a         2561 c         2093 g         1258 t
ORIGIN      
        1 cgtgccgact actccgtcgt ctggtggatc cgcgccgact ccgcggcgtc catcgcgcac
       61 tcgttcctca gcctttcagc ccgccttggc aaacagctgt cgcaggacgc gcccgccacg
      121 ctgatcatcg attcgctcaa gcaactgctc gcccggcgcg actggttgat gatcttcgac
      181 aacgccagcg gcgccgatgc catccggccg ttcatcatcg gcgaaggcaa cggtcacgtc
      241 ctgatcacca gccgaaaccc caactgggcc ggcgtggcgg tgtcgcagcc gctgaaggtg
      301 atgacgcgcg atgagtcgat cgaattcctc atgcgccgca cccgccgatc cgaaacgcgc
      361 gacgccgccg acaagctggc caacgcgctg ggtgacctcc cgctggcgct ggaacaagcc
      421 gccgcgacga tcgaacaaac cggcgtcacg tttgccgatt accttcggcg ttttgaaacg
      481 gagtgggccg acctgctgca ggaaggcgtg aagccggtcg attacccgca cacggtggcg
      541 atgacctggg gccttgcgtt caacgcggtc gaaaccgcgt ccgccgccgc ggcggagttg
      601 ctcaacttgt gcgcgtttct ctgtccggac cgaatcacgc ggaagatgct gtgggaaggc
      661 gaagaacacc tgccgccccg gctgcggaag ttcaccgacc cgctgcggtt ggaagacgcg
      721 atttcgccac tgcggaatta ttcgctggtc gatgccgacg atcgcaacat cagcatgcac
      781 cggctggtcg cgacgatcac gcgcgatcga ctggacgagg aacgccagat cgtctggtgc
      841 aacgcggcca tcgaattcct cagcgggatg tttcagttcg acagtgccaa cgtcgattcc
      901 tgggccaagt gcggcgaact gctgccgcac gtgatggacg cgtcaagcca cgcggatcga
      961 ttgaaggtcg cgccggacat cacgtcggaa ttgctgaacg acgcggggcg atacctgctg
     1021 aaacgcggcc aatatgctga cgccaaggcg gcgctggaca aggcgctggc gatctgctcc
     1081 cgccgctacg gcaccgggca tccgaagatt tcctcgatca ccaacaacct tggtcgggtg
     1141 caccaaaacc tcggcgacga cgccgcggcc atccaatgct tcgagcacgc gctgtcgatc
     1201 gacgaagcaa cgtacggcca ctcgcacccg cacgtggcgg aggtcatcaa caactacggc
     1261 atctgcctgc agaagaaggg ggacagcgaa ttggcccgcc aacaattcga gtgggctgcc
     1321 caggtgtacg agacgcatta tgggccggac catgcgaagc tggcccacat cctgaacaac
     1381 ctcggctacg tgctgatggc cagcggcaac atcgagtccg cccacccgca catcacgcgg
     1441 gcgctggaga tcgcggagaa aacggttggc ccgtcgcatc cgactacggc gcgcatcctc
     1501 tacaacctcg cgacggtcta tcgcgtcacc aaccaactgc ccgccgccaa ggagcacctc
     1561 gagcgcgcgc tgcgcatcga cgagacggta ctcggcccga atcaccccga cgtcgccaac
     1621 gactgcatcg cgatgtccga gttgctcaac aagatgggcc aagccgcgat ggccaacgcg
     1681 tattatgaac gcgccacgca aatccgtgcc aacagcggca gcccgccgcc gcagcgccag
     1741 ggcgcgcccg ccgcggtttc gatggtttcg tagtagtaac gacgacaatg aatatgatcc
     1801 cagaaaagcc cgcggctccg ccgtgggatt ttttttgcgc ccacgcgcgc ttccaccacc
     1861 taagatgatg cagctcgcac ctgatcgtgg cgcaagcttg aagccccgca tgtacatgct
     1921 gggctccgtc ggaaatggac cattcaatta cttctgagca agatccttcg actgcgcacg
     1981 agtacgtgcc gcgctcagga tgacaccggc attcgcgcgt tcattgaatt ttcttacggc
     2041 aaccgcgcta ttcccttccc taagaccctc cggataattt gctcgacatg atcctccgca
     2101 gcctgattgc atctttgacg atcaccctgc tcgcgagctg cgtgaattct ggcgcaggcg
     2161 caggcgccgt cgtgaaccca tcgttcccaa tctccgagcc cgccgcccga gacgtcattc
     2221 gcaacatcgc gcgccagcac agaccgctcc cccgcccgct cgtgatcatc ggcgggtttc
     2281 gcgaccccgg catcgccgcc ccgatcctga aacatgaatt cgccaagtgg accggcagcg
     2341 gcgatcgcat cgtcaccgtc tcgctcgcga tgaccttcag cttcgaccaa tgccgccaac
     2401 gcatcatcga cgcggtggat caggcatttc ccagcgacga tccacgccaa acgatcgagg
     2461 ttgacgtaat cggcttttcg atgggcggcc tcgccgcccg ctacgcggca tccgatgtcc
     2521 ccccggaaca gggtgaacca gcgacgcgcc gcctccgcat cgcccgcctg tttacgatca
     2581 gctcacccct gcgcggcgcc gccgttgccg cggagcttcc gctgaccctg catccgctgc
     2641 aaccgccgat gcgccccggc tcgcgcttcc tgcgcgcgat cgacgccgcc cccgttcggc
     2701 cggacgagct ttaccagatc ttctcgtacg tccggctcgg cgacccgtat atccgcgagg
     2761 ccaactccgc catcaaccgc gaaaccgcgt ggtgggtcag caccccgccg ctatccccgc
     2821 cgcacgagat ggcgtttcag gatggtcgca ttctcgccga catcctctgc cgtctccttg
     2881 gtgagccacc gctgacgagt gatccgccca ccccgctgcc caacccgcag caagccgccg
     2941 ccgccgcggc gcacgtgccc aatctcgaat gagcggttag gatgtcgccc gaagatcgtg
     3001 cagcgcaccg catccatcac ggcgtacatc ggcctcggct ccaacctcgg cgatcgcgcc
     3061 ggcaatttga acgcagccgc cgaacgcctc ggccagcccg atcacacgcg cgtcgtcgca
     3121 acatcatcgt tcctggaaaa tcccgcggtc ggtggaccgc ccgattcacc gccgttcctc
     3181 aacgccgcgg tgaaaatcga gacgacgctg ggctcgcacg cgctgttgca tcgactgctg
     3241 gagatcgagc gagaaatggg ccgcgaccgc cgggcgaaat gggagccgcg tacgatcgat
     3301 ctggacctgc tgttgtatgg cgatcaaatc atcagcagcc aggaattgat cgtcccccac
     3361 ccgctgatgc acgaacgccg gtttgtttta caaccgctgg ccgagatcgc gcccgacgtg
     3421 gtgcatccca tgctgcagat gacgatggcg gggttgctgg cgacggtgtg aggcggatca
     3481 cagcccatga cagccgcggg cgacagccag cggggccttt cagcgtcagg ccgcgttgat
     3541 cttccgaagc ccccgctggc tgtcgcctgc ggctataaag gatcgcagtt cgaacgtggt
     3601 tcacacgccc ccgcacgcgt gcatcccaat gtgccctcgc ccgcttcgct acgctgcccc
     3661 cacgccgaac tcgcgcttga aatcttccag cgacgcgacg cccgccggca gttttgcttc
     3721 ctcgatcagc atcaccggaa tgccttcgcg caccgggtaa ctcagcccgc cgacttcagc
     3781 gaccagccaa tccccctcct gtcgcaggcg gctgcgcgtc agcggacagc gcagaatctg
     3841 gagcaaatcc gcgtcaacca cgttcgatgt cttctcttcc gtcatgccaa tcgcacctta
     3901 taagaacaac cgcaaacaat acagcgcatc ggcgagcagc accaacgaaa cgccgtcgat
     3961 gcccgccccg ggcgcctgcg tggccgcggc gtgcagcacc tggtcggcca gcgacctcgt
     4021 cgccgcattc cacgcaaacg cgaagacgcc ccacggctgc gtcgtcgcgt ggtccggctg
     4081 tgtctcccgc agatgccact cgccattgtg cgccaccgcc gcggccaccg tgcggtcctc
     4141 cgcctgcacc gcataactcg ccgccgcgtg caggatcacc aattcgtgat accagtgcgt
     4201 ctccggatta tcgcgcgccg acgtggccag aaaactgccg ctgctttgct gcgcgcgcgt
     4261 gatcttgcca aacacatcgc cggccaggtc ggtccacgcg tcgcgcacga acgctttgcc
     4321 cgcgatgtgc agcgccagcg ccatccacgc cgcctccgcc agcctcgccc cgcgaccggc
     4381 ggggatgggg ccatcgaacc actcgacgtc gccgatcaaa ctttccagca gatcacacca
     4441 cggccgcagg ccttcctccc attgcccgaa cacatcgcgc ggcatcgtct cgtacgccag
     4501 gcgatacgcc tgcagccacg cgtaaatcag cagcccgcga tacaccggcc ggtgatgccc
     4561 gctgcgatcg acgaccgcga tgtcttgcag attggcaagc cgcgacagct cgacgagccg
     4621 gttcaccgat gtggcgcgct cggccgacca cccgccgttc agcgtcgatg gaaacacgat
     4681 cgcccgttcc acctggatgg accgagcatc ttccgcctcg gcggtgaaac ccggcggcgg
     4741 cgcggtcatc ggctcgatca gcgctgcgta ccgccgaagc tcgttttgaa tgccggtcgg
     4801 atcggtcatg cgaaaatagt gtagcggagg aagcggcgac cgcttgtgac gcacggcgaa
     4861 attttttgcc acgccgtcgc cccacgtaga tcatgttcgt tgcaatgacc accatcaccg
     4921 cacccgcgtc atctttgccg tcggatcatg ccgatctttc cgctggacgc ggatggttga
     4981 agcgcgtccc gctgttgacg ggcgtgctgg ccgcgctcgc gggatttctc accgttcgcg
     5041 gcgccaacct gtcgaaccag gcaatctatc acagcaatca gggcgtgctg caccaagcgc
     5101 gggcatccga tgcgtgggcg gagtatcagg cgaacagcat caaggcccgc atcgtcgaaa
     5161 cgacattgct gacagcaatc gataccgcct caaaggacgc gctgaacaca caggcaaaag
     5221 agctgcgcgt acgccagccc accattcgcg ccacggccga ggaccaggag cagcagcgcg
     5281 acgccgagtt gaccggtggc caacggcgac tggcagagaa ggacctgctc gattacgccg
     5341 gcatggccgc gcagctgggc atcgcgctgg cgtccgtcgc agccttgacc cgccgtcgcg
     5401 atgcgttcgc cgtcggcgtc gtctgcggcg ccctcggcgt cgccatcacc gcgtatgcga
     5461 tcgtcgtcaa ccacttcgtc cacgcctagt gatcaggcag tcctaaagcg cgcccgcgcc
     5521 tttccccctc gcccacgtac tcgtgggaga gggctggggt gagggtgatt taaaatttcc
     5581 aacgcggctc gacgttccaa atcaccctca ccctaaccct ctcccaagcg tacgtcggag
     5641 agggaaccag acacctcagc agccatgcgt tttatccggc cagcgatcac taatcgtcca
     5701 tcggcctcac cgccaaatcc cgcagcgtgg cggtccagct gacgcgctcg ccattcatga
     5761 tgaagcgcac cacggcttca gcggcgacga gcgtcgccag cagcaccagg ttccgcgcca
     5821 gcggataatc gcacacgtcg ttgccgacat cccgcggcac ccggtagcgc tcgtcccaga
     5881 ccacttcgcc gtagtcttca aacaacccca cgtgcaggca cggcgtcgcc gtcgcccgcg
     5941 cgtgatcctg cacgagctgc cgcgaagccg cgttgtcgaa cgcatcgacg atcagttccg
     6001 aatccttgag caactgcctg gcgttggccg ccgacagctc cttgcgcatg gcgccgatct
     6061 caacgccaac agtgcggaac aggcggttgc gcagcgcctc caccttccag acgccgacat
     6121 cgccttcgcc gtagatctgg gtcgaaacgt tatgctcttc aacgcggtcg tggtcgatga
     6181 cgcggagatg cgcgacgccc tgccgcgcca gcgcgtcggc gaggttggag ccgacggcgc
     6241 cagcgccgca gagggtgatg tgcgacgtgg ccagatgctc cagcagcgcg tccccgcggt
     6301 agagcgattc gtgatggaac ttgccgggca ttggattctc tctttcgcgc gatgatcccc
     6361 ccgacgccgg tgtctgaggt actccgaaga cccgggtctt cgcgcgtacg ctcagacacc
     6421 ggcgtcggag gtggcggcgc aaactcgcta tcggcgccgc tgattccaat atgtgcaacc
     6481 ccgcgcagca caccgaccag gctggcgaga tcgaacttcc ggtcctcgcc gctcaagcag
     6541 atgccagccg agtggaccgt caggtctcgc ttgtccacca cgctcacgtg ccgccggcca
     6601 tcgaccgtgt atgtgacgcg gaacccgtcg gccgtacgcc ggagggagtt gaaccctcat
     6661 cgcctgggtg caggccagga gtcttcccgt tagacgacgg gcccgttcga ctcgctgcgc
     6721 tcgctcaggg cacgcccagc gtagtcagcg ctgcgggaag ggatcgaacc ttcaacctcg
     6781 gccttcggag ggccgcgctc tgttccggtt gagctaccgc aacttagggt cggaatcagt
     6841 tgttggttgc ttgtttctag tggaaggcac aagcaccctc ttcactagca actaacaaca
     6901 agcaaccagc aactgatcct ccgaagtggt ccaggcagga ctcgaacctg cacttacctg
     6961 attctgagtc aggcgcctct gccgattggg ctactgaacc aacgttcgct ttctctccaa
     7021 tgcaccgggc gggactcgaa cccgcaccct gccgcttaaa aggcggcagc tctgccttcg
     7081 agctaccggt gcccccaatg caccgggagg gattcgaacc ctcacccgac cctttagaag
     7141 agggtggctc tgattccgtt gagctaccgg tgcgtcttca tttgcggcac cgttttcttc
     7201 tgccgatcat gttccacctc tgtctttact gacccctgac tcctgacccc ttccatgc
//
LOCUS       DATIMH010000677         3201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3629261, whole genome shotgun sequence.
ACCESSION   DATIMH010000677 DATIMH010000000
VERSION     DATIMH010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3629261"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VLI90_11775"
     CDS             <1..222
                     /locus_tag="VLI90_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14929.1"
                     /translation="IRRFPILSAMADTPGQTDKEWPCIEVLHHPPPQMGSFERGNRCA
                     IAASILPSYRNMRNVEDSIGGRVAGGSRR"
     gene            219..482
                     /locus_tag="VLI90_11780"
     CDS             219..482
                     /locus_tag="VLI90_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HSV14930.1"
                     /translation="MTGDVSQDQFALIKIEGMHCHKCEQSIQKSLLRNPGVHEVEVDF
                     ASGQASVLFDPSAVSVPQLLESVKEAGYRPTGFSQGQTDRARP"
     gene            487..939
                     /locus_tag="VLI90_11785"
     CDS             487..939
                     /locus_tag="VLI90_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14931.1"
                     /translation="MPPDPPLLANPTDTLEALLHRPIEQRAREFKYRFAQSVVFGLPV
                     IALQRYGASLGGPEAARWSGLLQLLLATWVIYVAAAGMLFEGLVTLRRKFSADLLIAL
                     AAACLYLFSVWSVARMLVNGSATHRPLFDAAIVLLATWTGIRYAQLAR"
     gene            complement(1073..1408)
                     /locus_tag="VLI90_11790"
     CDS             complement(1073..1408)
                     /locus_tag="VLI90_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14932.1"
                     /translation="MAGDANIAGKVDVADLGARATSYGVSTGGTWGQGDFEYNGNVGV
                     GDLGMLATNYGVSIAAGAVDASSTIAPVVVATPAAAVAVPEPIGGAFLLVSAMVISWR
                     RQRRSLFGR"
     gene            1548..1667
                     /locus_tag="VLI90_11795"
                     /pseudo
     CDS             1548..1667
                     /locus_tag="VLI90_11795"
                     /inference="COORDINATES: protein motif:HMM:NF024932.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1683..1970
                     /locus_tag="VLI90_11800"
     CDS             1683..1970
                     /locus_tag="VLI90_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSV14933.1"
                     /translation="MRGQERKEATVVKRIGVDEKAFRKGHSYLTVVCDLDRATVEHVS
                     EDRTIQSLSSYYTSLSPQQRDGIRTTNYRSAVRSSAPTCGAFLARFWWSLV"
     gene            1998..>3201
                     /locus_tag="VLI90_11805"
     CDS             1998..>3201
                     /locus_tag="VLI90_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSV14934.1"
                     /translation="MFEPLTQRRYLIPFKAARLPQQFTDVLVIGGGVAGLRAAIAAAD
                     GGVDVLVLTKDTIDESNTWYAQGGIAAVLQPMDSIESHIKDTEIGGAGLCDDKAVRIV
                     IEEGPQRVLELLEWGANFDKKIGNPYDLAFTREGGHSFARILHAYGDATGKELAQTLI
                     RTVRSRDNIRISERTFVIDLITEEGPATSAAGIGPKCLGVIALIDGKIQIIWAKRTIL
                     ASGGAGQLYRESTNPKIATADGHAMAYRAGARLQDMEMVQFHPTTLYVAGSSRALVTE
                     AVRGEGAYLVDRNGYRFMKDYHEMGELAPRDVVSRAIVEQIRKTHFTHVYLDVRHLPT
                     PAFRERFPSLAALVDQFEIDVSKDLIPIHPAAHYMIGGVDVDEHGCSSLDGLYAVGEA
                     SCTGLHGAN"
BASE COUNT          614 a         1053 c          967 g          567 t
ORIGIN      
        1 ataaggagat tcccgatttt gtcggcgatg gcagacacgc ccggtcaaac tgacaaagaa
       61 tggccatgca ttgaagtgct gcaccatccg ccaccgcaga tgggctcgtt tgaacgcgga
      121 aatcgctgcg ccatcgcagc atcgatattg ccaagttacc ggaacatgcg taacgttgag
      181 gactcgattg gcgggcgggt tgcaggaggt tcgaggcgat gaccggtgat gtgagccagg
      241 atcaatttgc cttgatcaag atcgaaggca tgcactgcca caagtgcgag cagtcgattc
      301 agaaatccct gctgcgcaac cccggcgtgc acgaagtcga agtcgatttc gccagcggcc
      361 aggcctcggt cctgttcgac ccctccgccg tctccgtccc gcagctgctc gaatcggtga
      421 aagaagccgg ctaccgcccc accggtttct cgcagggaca gacagaccgg gcgcgtccat
      481 aaacagatgc cgcccgaccc accccttctg gcgaatccca ccgatacgct cgaagcattg
      541 ttgcaccgcc caatcgaaca acgcgcgcgc gaattcaaat atcgcttcgc gcaatctgtc
      601 gtcttcggcc ttccggtcat cgcgctgcag cgctacggcg catccctcgg cggacccgaa
      661 gccgcccgat ggtccggcct ccttcaactg ctgcttgcaa cttgggtgat atacgtcgcc
      721 gccgcgggga tgttgttcga aggcttggtc acgctacgac ggaagttcag cgccgactta
      781 ctgattgccc tcgccgccgc gtgcctctat ctcttcagtg tctggtccgt agcgcgaatg
      841 ctcgtcaatg gcagcgcaac ccatcgcccc ctcttcgacg ccgcaatcgt tctgctcgca
      901 acttggaccg gcatccgata cgcgcagctc gcgcgctgat gacgattgct tcgagaacga
      961 gaggaaatac cccacgccgg gttcggagcg tactcgcgac gaagcgagat tcttcttggc
     1021 gccagaaagc atccgggtcg tcgcgagtac ctccgaaccg cggcgtggga cgttatcggc
     1081 caaacagtga acggcgctgg cgtcgccaag aaatgaccat tgcgctcacc aacaaaaatg
     1141 ctccaccaat gggctccggc actgccaccg cggccgcggg tgtcgccaca accaccggcg
     1201 caatcgtgct cgacgcatcc accgccccgg cggcgatact gacgccatag ttcgtcgcga
     1261 gcattccgag atcgcccacg cccacgttgc cgttgtattc gaaatcgccc tgcccccacg
     1321 tgccgccggt gctcacgccg tagcttgtcg cccgcgcgcc caggtcggcc acgtccacct
     1381 tgccagcgat gttcgcatcc cccgccaacg tattctcgcc tccaccaccc cgctgccaag
     1441 gtccagcgaa ccgattgctc cgcccgaggt ggacgtgaag atcccaccag agcgcccggt
     1501 aagggacgca accgcttccg tattcagatc ttcgcccgtg ggcccagacg cgcagccgtt
     1561 tcacgctgct gatggagcgg ttgatcatcg acgtgatcgg ccagtgctcg acgatcagcg
     1621 gggcctgcgc gatcatgcgg gtcagttggg acgaggcctt gggcgtgatg cagcgtgcgg
     1681 tgatgcgcgg gcaagagcgc aaggaggcga cggtggtgaa gcggatcggg gtggacgaga
     1741 aggccttccg caagggccac agctatttga cggtggtgtg cgacctggac cgcgccaccg
     1801 tcgagcacgt gtcggaggac cgcaccatcc aaagcctgag cagctactac acgtcgctct
     1861 cgccccagca gcgcgatgga atccgtacca ccaattaccg cagcgccgtt cgtagctccg
     1921 cgcctacatg cggggctttt ttggcgagat tttggtggtc ccttgtctga tcccgctctc
     1981 gatctaacat tgccggcatg ttcgagcccc tcacgcagcg gcggtatctc atcccattca
     2041 aagccgcgcg cttgccgcag cagttcacgg atgtattggt catcggcggc ggggtggcgg
     2101 gtcttcgcgc ggccatcgcc gccgcggatg gcggtgtgga cgttctcgtg ctgaccaagg
     2161 acacgatcga cgaatcgaat acgtggtatg cccagggcgg catcgccgcg gtgttgcagc
     2221 cgatggacag catcgagtcg cacatcaagg acaccgagat cggcggcgcc ggcttgtgcg
     2281 atgacaaggc cgtccgcatc gtcatcgaag aaggcccgca gcgcgtgctc gagctgctgg
     2341 aatggggcgc caacttcgac aaaaaaatcg gcaaccccta cgacctggcc ttcacgcgcg
     2401 aaggcggtca tagtttcgcc cgcatcctcc acgcctatgg cgacgccacg ggcaaagaac
     2461 tcgcccaaac gctcatccgc accgtgcgca gccgcgacaa catccgcatc agcgagcgca
     2521 cgttcgtgat cgacctcatt acggaagaag gccccgccac ctcagcggcc gggataggcc
     2581 ccaaatgcct cggcgtcatc gcgctcattg atggcaaaat ccagatcatc tgggccaagc
     2641 gcacgatcct cgccagcggt ggcgccggcc agctctatcg cgaatcgacc aacccaaaga
     2701 tcgcgacggc cgacggtcac gcaatggcgt atcgcgccgg cgcgcgattg caggacatgg
     2761 agatggtgca gttccatccg acgacgctgt acgtcgccgg gtcgagccgc gcgctggtca
     2821 ccgaagcggt gcgcggcgaa ggggcgtatc tggtcgatcg caacggctat cgcttcatga
     2881 aggattacca cgagatgggc gagctggccc cgcgcgacgt ggtgagccgg gcgatcgtcg
     2941 agcagatccg caagacgcac ttcacgcacg tgtacctgga cgttcgccac ctgccgacgc
     3001 cggcgttccg cgaacgcttc ccgtcgctgg cggcgctggt cgaccagttc gagatcgacg
     3061 tgagcaaaga cctcattccg attcatccgg ccgcgcatta catgatcggc ggcgtcgatg
     3121 tggacgagca cgggtgctcg agcctcgatg ggttgtatgc ggtcggtgaa gccagctgca
     3181 ccgggttaca cggggcgaat c
//
LOCUS       DATIMH010000678         3751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2183679, whole genome shotgun sequence.
ACCESSION   DATIMH010000678 DATIMH010000000
VERSION     DATIMH010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183679"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1850
                     /locus_tag="VLI90_11810"
     CDS             81..1850
                     /locus_tag="VLI90_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14935.1"
                     /translation="MPSANADNVVDEPPIAPPPPHAASVPPIRAVSSRRFGLIWLLVL
                     AGFAAFYPLAALAWVNFYRSPDIAFAELVYRQKDVLCQRQPSPRLLIVGGSGVHFSID
                     AALIEARLGIPSINYGSYAGLGFRYMLQRAQRQAHRGDTVLLSPEYSCWGRDAEHSND
                     PGFEYIWTYDQRAFYERPLLERASMIASIRLVDWVDAAKGWGARLRGKQYHFADIMLY
                     NVASDDEHGDVHVVLRPHNIPRQTGYGFPEQSMVSVTALRDFAAWAKANGVRVLFTWP
                     AFARPQPTVSKEASAPPGWFTDLLRDGGVTMLDQPADNTYPPQWFQDTEYHVTPACRR
                     LRTEELLRRLRPALNLPARPMGNDVLIVGPRDHHLNPGNTFATGPEVSVRILSDPAVE
                     DARAITPAGALDLARGGTNVYVGHDDAVAAGLSTQLSAHERMSIAEWFHRYPSHVLLI
                     AAAPGHRIDTSWQSAVPPAVYQALSSGSPTVAAFGTGRYAGTLKIVADATVAKLPAKL
                     PALVKPASALIALVSLESSASGAKINLDYGDIRTSDHGVCVAAVDPELGSAIDMATFT
                     DAPDVETWRLDRVVPGVATPTTR"
     gene            2050..3480
                     /locus_tag="VLI90_11815"
     CDS             2050..3480
                     /locus_tag="VLI90_11815"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HSV14936.1"
                     /translation="MLYNSFQFLFVFLPACLLLYFLAARVSRDLANVALCVASFYFYA
                     WRKDWSVDLRLLTIIAISILLNYTIGVLLREQRVAGRRAFAVLTVGVVVNLAALAYFK
                     YANFALWNISALMGRPYAPLAIALPLGISFFTFTQIAFLVDAYRGKARELGFARYCLF
                     VAFFPHLIAGPIVHHSELMPQFAATGAKRWLPPNVHIGIAFLTLGLFKKVIIADTCAP
                     WADKVFDFAGSVPFVDAWRGAIAYTMQLYFDFSGYSDMAIGLAMMFNIRLPDNFFSPY
                     KSVSIVDFWRRWHITLSRFLRDYLYIPLGGNRKGEARRQLNLMLTMLLGGIWHGAGWT
                     FAAWGAYHGALLTVNHAWEKRGKPLPNWLARFATFIAVIIGWVMFRASSMRVAADRLR
                     SMAGFAPASAAPPRPWSMVSGQVAILVILLFFVNLAPNTKQWIETRPLIPRRAVLLAA
                     LFFLCVLWMHDALLSHAPQPFIYFQF"
BASE COUNT          609 a         1239 c         1155 g          748 t
ORIGIN      
        1 cctgtgctac agtttgatct gccggcctcg ccgatataat cccgccgcaa cacgtaagcc
       61 cgaggagatg tttggaacgg atgccgtcag cgaacgccga caacgttgtc gatgaaccac
      121 cgatcgcgcc gccgccgccg cacgcggctt cggtgccgcc gattcgcgcc gtctcttctc
      181 gccgatttgg gctgatctgg ttgctcgtgc tcgccggctt cgccgcgttt tatccgctgg
      241 cggcgctggc gtgggtgaac ttttatcgca gccccgacat tgcgttcgcc gagctggtct
      301 atcgccagaa ggacgtgctc tgccaacggc agccgtcgcc gcggctgctc atcgtcggtg
      361 gctcgggtgt gcacttcagc atcgacgccg ctctgatcga agcgcggctc ggcatcccgt
      421 cgatcaacta cggcagctac gccggcctgg gattccggta catgctgcag cgcgcccagc
      481 ggcaagccca ccgcggcgac accgtgctgc tgtcgccgga gtacagctgc tggggccgcg
      541 acgccgagca ctcgaacgat ccgggtttcg agtacatctg gacctacgac cagcgcgcgt
      601 tttacgagcg gccgctgctt gagcgggcgt cgatgatcgc atcgatccgc ctggtcgatt
      661 gggtggatgc cgccaaaggt tggggtgcgc ggctcagggg caagcagtat cacttcgcgg
      721 acatcatgct gtacaacgtg gcgagcgacg acgagcacgg cgacgtgcac gtggttcttc
      781 ggccgcacaa catcccgcgc cagaccggct atggatttcc cgagcaatcg atggtgtcgg
      841 tgaccgcgct gcgcgacttc gccgcgtggg cgaaggccaa cggcgtgcgc gtgctgttca
      901 cctggccggc gttcgcgcgg ccacagccaa cggtatcgaa agaagcgagc gcgccacccg
      961 gttggttcac cgatctgctg cgcgatggcg gcgtgacgat gctggatcaa ccggcggaca
     1021 acacgtatcc gccgcagtgg tttcaggaca cggaatatca cgtgacgccc gcgtgtcggc
     1081 ggttgcggac ggaggaattg ctgcggcggt tgcggccggc gctgaacttg ccggcgcggc
     1141 cgatgggcaa tgatgttttg atcgttggcc cgcgcgatca tcacctcaat cccggcaaca
     1201 cgttcgctac cgggcctgaa gtgtcggtgc gcatcttgtc cgaccccgct gtcgaagacg
     1261 cgcgcgcgat cacgcccgcc ggagcgttgg atctcgcgcg cggcgggacg aacgtctacg
     1321 tcggtcatga tgatgctgtc gccgcggggc tttcgactca gttgtccgcg cacgagcgga
     1381 tgtcgatcgc tgaatggttc caccgttatc catcgcacgt gctgctgatc gccgccgcgc
     1441 cggggcatcg gatcgacaca agttggcaat cggccgtgcc gccggcggtg tatcaggcgc
     1501 tctcgagcgg atcgccgacg gtcgccgcgt tcggcaccgg gcgatacgcg ggcacgttga
     1561 agattgtcgc cgatgcgact gtcgcgaagc tgccggcgaa gctgccggcg ctggtgaaac
     1621 cggcgagcgc gctgatcgcg ctcgtgtcgc tggaaagcag cgcgtccggc gcgaagatca
     1681 acctcgacta cggcgacatc cgcaccagcg accacggcgt gtgcgtcgcc gcggtcgacc
     1741 cggaactggg ctcggcgatc gacatggcaa cgttcaccga tgcgccggac gtcgagacat
     1801 ggaggctgga ccgtgtggtg ccgggggtcg ccacgccgac aacgcggtaa cgggctgaaa
     1861 ctatcgagtg catcctcctt ctggtcccct cgcccatgta gtcatgggag agggtcaggg
     1921 tgagggtgat ttagattcga aagccgttgg tgctcgaaat caccctcacc ccagccctct
     1981 cccggtgtac cgggagaggg agtaagagcg gattcgccgc tcggcgcgcg atgatcgata
     2041 aaaaaccgtt tgctctacaa cagcttccaa tttctcttcg tcttcctgcc cgcgtgcctg
     2101 ctgctgtatt tcctggctgc ccgcgtctcg cgcgacctcg ccaacgtcgc gctctgcgtc
     2161 gcgtccttct acttctacgc ctggcgaaaa gactggtcgg tcgatcttcg cctgctgacg
     2221 atcatcgcga tcagcatcct gctgaattac acgatcggcg tgctgctgcg cgagcagcgc
     2281 gtcgccgggc ggcgcgcctt cgccgtgctc acggtcggcg tggtcgtaaa cctcgccgcg
     2341 ctggcgtact tcaagtacgc caacttcgcc ctgtggaaca tcagcgccct gatgggccgg
     2401 ccctacgcgc cgctggcaat cgcgctgccg ctcggcatct ccttcttcac cttcacgcag
     2461 atcgcatttc tagtcgatgc ctatcgcggc aaggcgcgcg agctgggctt tgcccgctat
     2521 tgcctgttcg tcgcgttttt cccgcatcta atcgccgggc cgatcgtgca tcacagcgag
     2581 ttgatgccgc agttcgccgc caccggcgcc aagcgctggc tgccgcccaa cgtgcacatc
     2641 ggcatcgcct ttttaacgct ggggctgttc aagaaggtca tcatcgccga cacctgcgcc
     2701 ccctgggccg acaaggtctt cgacttcgcc ggcagcgtgc cgttcgtcga tgcctggcgc
     2761 ggcgccatcg cttacacgat gcagctgtac ttcgacttca gcggatacag cgacatggcg
     2821 atcggcctgg cgatgatgtt caacatccgc ctgcccgaca atttcttcag cccctacaaa
     2881 tccgtcagca tcgtcgattt ctggcgccgc tggcacatca cgctgtcgcg cttcctgcgg
     2941 gattacctct acatcccgct cggcgggaat cgcaaaggcg aagcccgccg gcagctgaat
     3001 ctgatgctga ccatgctgct cggcggcatc tggcacggcg ccggttggac cttcgccgcc
     3061 tggggcgcgt atcacggcgc gctattaacg gtgaaccacg cctgggaaaa acgcggtaaa
     3121 ccgttaccga attggctcgc gcggttcgcc acgttcattg ccgtcatcat cggttgggtg
     3181 atgttccgcg cctccagcat gcgcgtcgcc gccgatcgcc tgcgcagcat ggccggcttc
     3241 gccccggcgt cggccgcgcc accgcgcccg tggagcatgg tcagcggcca ggtcgccatc
     3301 ctagtcatcc ttctgttttt cgtgaacctg gcccccaaca ccaagcaatg gatcgaaacc
     3361 cgcccgctga tcccccgccg agcagtgctg ctggcggcgc tgttcttttt atgcgtgctg
     3421 tggatgcacg atgccctgct gtcgcatgcc ccgcagccat ttatttactt tcagttctga
     3481 agccccaggt attctcttat ccgtcttgcc cgaaacggaa aatcactggg ccgtgcgcca
     3541 ttcaaactat gatggtaatt tgggaatgtt cctagcagtt ggcggggcaa taggttgtgt
     3601 cgatccccgc aggaacgatg cgtcttcaac cacgagttgc tgctgcctaa cttcacacga
     3661 aaccgcgggc gccaccctcg ataccaaaat cactcagatc gcaatcatcc cgcccgcgtt
     3721 tagccgccgc ggcctcgcgg cgcggattta g
//
LOCUS       DATIMH010000679         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_452963, whole genome shotgun sequence.
ACCESSION   DATIMH010000679 DATIMH010000000
VERSION     DATIMH010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452963"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VLI90_11820"
     CDS             <1..995
                     /locus_tag="VLI90_11820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSV14937.1"
                     /translation="QVALAQTNFQRTEQAVKGNAVSQQEYDTAKAQLEQAQAVLAGDK
                     AAAEQSRLNLEWCRVTAPIDGRVSNKLVTPGNLVNGGAGQATLLTTITSLDPMYCYVD
                     VDERSVLKYQELSREKKRVSARDSRIPCFLQLSNETNFPHEGVVDFVDNHIDPATGTI
                     RARGVYANPNGYLTPGMFGRVRIPGSGRYRTVLIPDAAIGTDQNIRYVLLVGTDDVVQ
                     SRPVTLGPLFGGLRSIEKGLSVNDEVVINGLLRARPGTKVAPQWSTINIDMGMLTAPG
                     SPTTQSLPATRSIPSTVPTNPDAAAAELHATSQPSTASAPPPPPPPPATEGAAK"
     gene            992..>2709
                     /locus_tag="VLI90_11825"
     CDS             992..>2709
                     /locus_tag="VLI90_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV14938.1"
                     /translation="MNFAHFFIDRPVFAGVISIVTVIVGSIAIFFLPIAQYPEIAPPT
                     VTVTANYPGASAKTIAETVATPIEEQVNGVENMLYMSSQSTNTGTMRLTIAFRLGTNL
                     DIAQVQVQNRVAIAQPQLPSDVTALGVVVRKASPDITLVIQVYSPDGRLDPLYLSNYA
                     TLQIRDEIARQPGVGDMNVFGARDYSMRLWLDPNKLSSRGITAGDVATAIREQNVQVA
                     AGIVGGPPLPPGTTPFQYTVNAQGRLTDPDQFANIVVKVGNDGRITRVRDVARVELGA
                     ADYTTANYFNGKPAVGMAVFQLPGSNSIQTANAIYAKMEQLKKSFPQGLDYSIPYDTT
                     LFVRASIRDVVFTLFEAIGLVVLVVLVFLQGWRPAIIPLLAIPVSLIGTFAVMAVAGF
                     SLNNLSLFGLVLAIGIVVDDAIVVVENVERWIEEGLAPREATYRAMAEVTPAVIAIAF
                     GLSAVFVPVAFITGIVGQFFRQFALTIATSTLISAFNSLTLFGLVLAIGIVVDDAIVI
                     VENASHHIEKGEAPREATIRAMNEVTGPVIAITLVLMAVFVPTAFLPGITGQLYRQFA
                     LTIAATA"
BASE COUNT          488 a          895 c          855 g          471 t
ORIGIN      
        1 cgcaagttgc gctggcgcag acgaacttcc agcgcaccga gcaggcggtg aagggcaatg
       61 cggtttcgca acaggagtat gacaccgcca aggcgcagct ggagcaggca caagccgtcc
      121 tcgccggtga caaggcggcg gcggagcagt cgcgcttgaa cctggaatgg tgccgggtca
      181 ccgcgccgat cgacggccgg gtgagcaaca agctggtgac gccggggaac ttggtcaacg
      241 gcggagccgg tcaggcgacg ctgttgacca ccatcacgtc gctggacccg atgtactgct
      301 acgtggatgt agacgagcga tcggtgctga aatatcagga gctgtcgcgc gagaagaagc
      361 gcgtcagcgc ccgcgactcg cgcatccctt gcttccttca gctgtccaac gaaacgaact
      421 tcccgcacga aggcgtggtc gatttcgttg acaatcacat cgatcccgcc acgggcacga
      481 ttcgcgctcg cggggtatac gccaacccaa atggctatct cacgcccggc atgttcggcc
      541 gcgtacgcat tcccggcagc gggcggtatc gcaccgtgct gattcccgac gcggccatcg
      601 gcaccgatca gaacatccgc tacgtgctgc tggtcggcac cgacgacgtc gtgcaaagcc
      661 ggccggtcac gctcggcccg ctcttcggcg gcttgcggtc gatcgagaag gggctgagcg
      721 tgaacgacga agtcgtcatc aatggtttgc tgcgcgcacg ccccggcacc aaggtcgcgc
      781 cgcagtggag cacgatcaac atcgacatgg gtatgttgac tgcgcccggc tcgccgacca
      841 cgcaaagcct gccggcaacg cgatcgattc cgagcaccgt ccccaccaac ccagacgccg
      901 ccgccgccga attgcacgcg acgtcgcaac catcgactgc atccgcgcca ccgcccccgc
      961 caccaccgcc ggcaacggag ggcgcggcga aatgaatttc gcgcacttct tcattgaccg
     1021 cccggtcttc gccggtgtca tctcgatcgt gacggtcatc gtcggctcga tcgccatctt
     1081 cttcctgccg atcgcccaat accccgagat cgccccgccc acggtgacgg tgactgcgaa
     1141 ctatcccggc gccagcgcca agaccatcgc cgagacggtg gcgaccccga tcgaagagca
     1201 ggtgaacggc gtcgagaaca tgctgtacat gtcgagccag agcacgaaca ccggcacgat
     1261 gcgattgacg attgcgtttc ggctggggac gaacctggac atcgcgcagg tgcaggtgca
     1321 gaaccgcgtg gcgatcgcgc agccacagct gcccagcgat gtcacggcgc tgggcgtcgt
     1381 cgtccgcaag gcgtcgccag atatcacgct ggtgatccaa gtctactcgc ccgatggccg
     1441 gctcgacccg ctgtacctga gcaactacgc gacgctgcag attcgcgatg agatcgcccg
     1501 ccagcccggc gtgggtgaca tgaacgtctt cggcgcacgc gattattcga tgcggctgtg
     1561 gctcgatccg aacaagctgt cgtcgcgcgg catcaccgcc ggcgacgtgg ccaccgccat
     1621 tcgcgaacag aacgtgcagg tggccgcggg catcgtcggt ggtccaccgc tgccaccggg
     1681 caccacgccg tttcaataca ccgtgaacgc gcaaggccgg ctgaccgatc ccgatcagtt
     1741 tgccaacatc gtcgtgaagg tgggcaacga tggccggatc acgcgcgtgc gcgatgttgc
     1801 gcgcgttgaa ttgggcgctg ccgactacac caccgcgaac tatttcaacg gcaaacccgc
     1861 cgtgggcatg gcggtgttcc agctgccggg atcgaattcg atccaaaccg ccaacgccat
     1921 ttacgcgaag atggagcagc tgaagaagag ttttccgcag gggctggatt acagcattcc
     1981 gtatgacacc acgctgttcg tccgcgccag cattcgcgac gtcgtcttca cgctcttcga
     2041 agcgatcggc ctggtcgtcc tcgtcgtgct ggtcttcctg caaggctggc ggccggcgat
     2101 cattccgctg ctggcgattc cggtctcctt gattggcacc ttcgccgtga tggcggtcgc
     2161 ggggttttcg ctgaacaacc tctcgctgtt cggacttgtc ctggccatcg gcatcgtggt
     2221 ggacgacgcg atcgtcgtgg tcgagaacgt cgagcggtgg atcgaagaag gactggcccc
     2281 gcgcgaagcc acataccgcg cgatggccga ggtgactcca gccgtcatcg cgattgcgtt
     2341 cggcctgtcg gcggtgttcg tgccggtcgc gttcatcacg gggatcgtgg gtcagttctt
     2401 ccggcagttc gccctgacga tcgcgacgtc gacgctgatc tcggcgttca actcgctgac
     2461 gctgttcggg ctggtcctgg ccatcggcat cgtggtggac gacgcgatcg tgatcgtgga
     2521 gaacgcctcc caccacatcg agaaggggga ggccccgcgc gaggcgacca tccgcgccat
     2581 gaacgaggtg accgggccgg tcatcgccat cacgctggtg ctgatggcgg tgttcgtccc
     2641 gacggcgttc ctgccgggga tcaccggcca actctaccgc cagttcgccc tgaccatcgc
     2701 ggccacggc
//
LOCUS       DATIMH010000680        10425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_163514, whole genome shotgun sequence.
ACCESSION   DATIMH010000680 DATIMH010000000
VERSION     DATIMH010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163514"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VLI90_11830"
     CDS             <1..191
                     /locus_tag="VLI90_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14939.1"
                     /translation="ERTISWLNRCRAILIRWDKKAENYLGLIQLACALLWYRRRCRLP
                     FGRLLEGLKIPSNRGDKI"
     gene            complement(173..246)
                     /locus_tag="VLI90_11835"
     tRNA            complement(173..246)
                     /locus_tag="VLI90_11835"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Pro,seq:cgg)
     gene            complement(378..1169)
                     /locus_tag="VLI90_11840"
     CDS             complement(378..1169)
                     /locus_tag="VLI90_11840"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSV14940.1"
                     /translation="MAKWHAPLVLGALLTQLALVGCQQQTPPVATLPPPSFDAPRLAM
                     ARPPAPVATPRPLPNIAVLPPAPKAITPPKSPALAGIPREWIPPVAPRDWRAIVVHHS
                     ATTTGGAAAFDKMHRAKGWDELGYDFVIGNGTDTADGQIEVGPRWAKQKYGAHDKTPD
                     NWYNEHGIGICLVGNFDVERPTPKQMQSLTKLVTYLMHTYHITPDHLVGHGETKSTEC
                     PGKYMNIAQLRRQEARIVSATDGVQATQAAIAPDKDEELLTATTR"
     gene            complement(1162..1386)
                     /locus_tag="VLI90_11845"
     CDS             complement(1162..1386)
                     /locus_tag="VLI90_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14941.1"
                     /translation="MNLKANANCRGAAGVFSCAAFAAAAAHFCRCYWKTHTPSDKKCI
                     GDMAVAQAFDETFVERRSTRCTFTEEAKDG"
     gene            complement(1488..2048)
                     /locus_tag="VLI90_11850"
     CDS             complement(1488..2048)
                     /locus_tag="VLI90_11850"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSV14942.1"
                     /translation="MIPVLIVVGVLFVGVLVVMSAYNGLVSLREQVRGAWAQIDVLLK
                     RRYDLIPNLVETVKGYAAHEKSTLEAVIAARNQAVAVSGNPAQLSQAEGQLTGALRQL
                     FAVAEAYPDLKANANFMELQRELTGTENVIGQTRQHYNSLVASYNTAILSFPNNLLAG
                     PFGFHPQPFFEIQDAAQREAPVVKFT"
     gene            complement(2297..2695)
                     /locus_tag="VLI90_11855"
     CDS             complement(2297..2695)
                     /locus_tag="VLI90_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14943.1"
                     /translation="MTSRIFSALVLAAVLAVLGLLVIGCDSMTDYPHNGEQTDRSDTL
                     KTGSGTYAGTDSAYEAGNPAGNGATTAGITGSPGMDTGGANSAGHTGNMGNRSGTGMT
                     APGTGTYGGGNVGGSNAGNTGTTVPNTERR"
     gene            complement(2738..3142)
                     /locus_tag="VLI90_11860"
     CDS             complement(2738..3142)
                     /locus_tag="VLI90_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14944.1"
                     /translation="MNARRRTYRRSAALIHDILLRLALGTGLLTPALALAVDDQPTTA
                     PTTQSSSEFAGGRSLSSRDPAMSTTRPGNKPAMVAPVEGATGSNGDMPVDAQVGTGIG
                     PSQGDRVPNAAVATRPTRAAPPPSPPVGQSAK"
     gene            complement(3428..4006)
                     /locus_tag="VLI90_11865"
     CDS             complement(3428..4006)
                     /locus_tag="VLI90_11865"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSV14945.1"
                     /translation="MIARSPRGVAPVAGLDSRYYITFEVKESVMEMESLQDLFVDQLK
                     DLYNAENQIIKALPKMIKKASAPELKDAFARHLEVTKGQVDRLEQVFEAFGEKAKGKT
                     CKAMKGIIEEASDTMKEDAEPEVLDAALIAECQRVEHYEIAGYGTVRAYAELLGNKQA
                     AKLLQQTLDEEGEADKKLTSLAETLINVEAQA"
     gene            4107..6428
                     /locus_tag="VLI90_11870"
     CDS             4107..6428
                     /locus_tag="VLI90_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HSV14946.1"
                     /translation="MTLRPRPIALPIALFLSALLSLLLAPFALADGLIIIRNGPVVPG
                     HFAFAPLEVTYHHVDVRIDDQVATTSVDEEFYNPNGQRLEGEYIFPLPAGSHIDNFSM
                     DVNGQMMTAELLPADKAKQLYEDIVRQMRDPALLEYTGRDALKVHIFPIEPNSRKHVQ
                     LKYTQLLSSDAGVVEYSYPLNTEKFSSRPLNDVSVRVTLACKQPIKSIYSPSHNVDIS
                     RHGDRDATVGYEAKDVRPDMDFRLIFSRKQTPLGIDLMTYKLGSSDGYFMVLASPGAD
                     DPGKTVTIPKDVCFVLDTSGSMAGPKLEQAKKAMNFCLANLNPEDRFEVIRFSTEPEA
                     LFNGLEPVNGENSSKARAFVENLKAIGGTAIDEALRKALELRRPQNVARDQYSEAMIK
                     EDGRPFVVIFLTDGLPTVGETREDPIVAHASQEAQSRKGRVFCFGIGNDVNTHLLDRI
                     AGDTRAASQYVGPEEDIEVKVSNFYSKIKEPAMTDVQLAVTGSNVRTSQLYPNVMPDL
                     FKGQMLVAFGRYSGDGAAAVKVSGIVNGAHVEFAQDVTFATENINNAFIPRLWAARRV
                     GWLLDEIRLHGESPELKDEVTRLAREHGIVTPYTAYLIMEDERKRSVPLSLQSYREMS
                     TDTAAAGAGKVYFDSMDDEAKKESSRSGRQAVANAGNLAQLKDSQNLSQSTVEAPMQK
                     TPTAGGGGFGYRNTTNYAQQVRVMNGRAFYQNGNTWSDGTAQSQQNLKQKKIAFNSDE
                     YFDLLKQHPEAVSWLSLGNEVDVVLDDTLIMVR"
     gene            6600..7148
                     /locus_tag="VLI90_11875"
     CDS             6600..7148
                     /locus_tag="VLI90_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HSV14947.1"
                     /translation="MIHPWHDVTPGEDIPQEYNSVIEIPFGSSVKYELDKSSGLIKLD
                     RVLYSAVYYPANYGFVPQTLAEDDDPLDVLVLCQETVVPLTLIHARTIGLMTMIDGGK
                     KDHKIIAVATEDPEFNSYREAAEMPAHRLTMLRRFFQDYKQLEGKAVEVDEIQPAKVA
                     FPIIEDALARYSRQRRKGFGGK"
     gene            7218..7403
                     /locus_tag="VLI90_11880"
     CDS             7218..7403
                     /locus_tag="VLI90_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14948.1"
                     /translation="MAIRSSTTRYVPSDEPVIAQVRAALPRGRQASLLVVSAGGNDGL
                     ASSYILGQRACHPSSPR"
     gene            7379..7570
                     /locus_tag="VLI90_11885"
     CDS             7379..7570
                     /locus_tag="VLI90_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14949.1"
                     /translation="MSSVVAALKHVQDIPLGFSDTYPKMIGKLRTTNLPATVCRVDDA
                     VPGFGAAELKSKSDWSWRR"
     gene            7924..9252
                     /locus_tag="VLI90_11890"
     CDS             7924..9252
                     /locus_tag="VLI90_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HSV14950.1"
                     /translation="MRHRKVIAALATLPVLGLVGGLGYYAVKNRQIEADLNAAVARAE
                     ASKPVQVDFAVSVPGDTPKDQIIYLSGSVPALGNWDAAGVPMQRQDDGKWHAKVTVLS
                     GIDYGYKVTRGTWGTVETDAKGAAIADHSLNVEGPRAMDVSVAEWIDHGQAVPGRVTM
                     TGDLRLTKNFPSKLLNNTRTLIVYLPPGYDQNTADRYPVLYMQDGQNLFDEATSFAGV
                     EWKMDEAAQALIGQGEASPFIVVGIYNTPQRTGEFTPPTMADAAGSASDHTPAHGDLY
                     ARFVIEEVKPFIDQTYRTQTDRAHTLIGGAALGGLEALYTAQSHSDIFGQVIALSPWL
                     RQNDKKLVPQFVGDGKWLHSERLYASMGTDPGDNGNNYPGGPTAAVADGAELAQALKS
                     AGLQPDKDFVYREIKGGHQDEPSWQAEIGDVLKWVFARPATESTTTPAVE"
     gene            9520..10119
                     /locus_tag="VLI90_11895"
     CDS             9520..10119
                     /locus_tag="VLI90_11895"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HSV14951.1"
                     /translation="MAWAYYLLLIASDIVGLLLAAFTLPGLWLMLAAAAGYAFLTHGQ
                     YLSYKTLIALLVLAAIAEIAEFVLGGAGAKKAGASKWGIVGGLVGAILGGLFLTGVIP
                     IFAPLSTIIGICLGSFAGAFGIELALGRPIMQSVNIGYGAAKGRLLGIANKVAVGVVM
                     FLITLWVALPIHGTQPPPATASPPVNGAAPTTATTTPSP"
BASE COUNT         1934 a         3328 c         3229 g         1934 t
ORIGIN      
        1 tggagcggac gatctcatgg ctcaaccgct gccgggcgat cctgatccgc tgggacaaga
       61 aggcggagaa ctatctgggc cttatccaac tcgcttgcgc gttgctatgg tatcgacgcc
      121 gctgccggct gcccttcggg cggctgctcg aagggctaaa aatcccatct aatcggggtg
      181 acaagatttg aacttgcggc ctcttggtcc cgaaccaagc gctctaccaa gctgagctac
      241 accccgggat gccgcttttt gtttcagcca agcggcaaga cacatcagtg tagccgtcgg
      301 tgcaggggtt tcaagggctg ctcgctactg ctcgcttctg ctcgctctcg tgttctgcgc
      361 tcacggcccc gtcgagctta ccgcgtcgtc gcggtcagca gttcttcgtc cttgtcgggg
      421 gcgatggcgg cttgcgtcgc ctgcacgcca tcggtggcgc tgacgatgcg agcctcctgc
      481 cggcggagtt gtgcgatgtt catgtacttg ccggggcatt ccgtgctctt ggtctcgccg
      541 tgaccgacca ggtggtccgg cgtgatgtgg tacgtgtgca tcaggtacgt caccagcttg
      601 gtcagcgact gcatctgctt gggcgtgggc cgttcgacgt cgaaattgcc gaccaggcag
      661 atgccgatgc cgtgttcgtt gtaccaattg tccggcgtct tatcgtgggc gccatatttc
      721 tgcttggccc agcgcgggcc gacttcgatc tggccgtcgg cggtgtcggt gccgttgccg
      781 atgacgaagt cgtaacccag ttcatcccag cctttggcgc gatgcatctt atcgaaggcg
      841 gcggcgccgc cggtggttgt ggcgctgtgg tgtacgacga tcgcgcgcca atcgcgcggc
      901 gcaaccggcg gaatccactc gcgcggaatg ccggccaagg cgggcgactt tggcggggtg
      961 atcgccttgg gcgcgggcgg cagcacggcg atgttcggca aaggccgcgg cgtcgccacc
     1021 ggcgccggtg ggcgggccat cgccaggcga ggcgcatcaa aactgggggg agggagcgtt
     1081 gcgaccgggg gggtctgctg ctgacaaccg accaatgcca actgcgtcag caaagcgccc
     1141 aagacaaggg gagcgtgcca cttagccatc cttggcctct tccgtgaagg tgcaccgcgt
     1201 ggaccggcgt tcgacgaacg tttcgtcaaa cgcttgagcg accgccatgt cgccgatgca
     1261 ttttttatcg gaaggcgtgt gcgttttcca gtagcaacgg caaaaatgcg ccgccgcagc
     1321 ggcgaatgcc gcgcaagaga aaacgccagc tgcccccctg cagttggcgt tcgctttcag
     1381 gttcacggat accgatctgg ctcgctcagt cgggaaaagt tagggaatct tctgcgggtt
     1441 tatcaattga cctaaatagg ttagtcgccg gaattccgct tccggaatca tgtgaatttg
     1501 accaccggcg cctcgcgctg ggcggcatcc tgaatttcga agaacggctg cgggtggaat
     1561 ccgaacggac cggccagcag gttgttgggg aagctgagga tcgcggtgtt gtacgacgcg
     1621 accagcgagt tgtaatgctg gcgcgtctgg ccgatcacgt tttcggtgcc ggtcagctcg
     1681 cgctgcagct ccatgaagtt cgcgttcgct ttcaagtcgg gatacgcctc ggccaccgcg
     1741 aacaactgcc gaagcgcgcc ggtcagctgc ccttccgctt gtgacaactg tgctggattc
     1801 cccgacaccg ccaccgcctg atttcgcgcg gcgatcaccg cttccagcgt ggacttctca
     1861 tgggccgcgt atcccttcac ggtttcgacc aggttcggga tcaggtcata ccgccgcttg
     1921 agcagcacat cgatctgcgc ccacgccccc cgcacttgct cgcgcagcga gaccaggccg
     1981 ttgtaggccg acatgacgac gaggacgccg acgaatagaa cgccaacgac gatgaggacg
     2041 gggatcattc aaagctcctt cgggcgagaa ttgtgcaagg ccgccggggg gacggctatt
     2101 agaactttgg atgatcttgc caatcggcta tagaaggggc aagcggccgg acgttgatga
     2161 gcgcgagatg tgttgaagct ttccgccgcg tagcggtagc cccgcagggg cacgtggttt
     2221 ggtgcgcgaa tcaaaaccac gtgcccctgc ggggctaccg ctacgcgggg atccgctacg
     2281 cggggatcag gcgcgctcag cgccgctcag tattcggcac ggtcgtgccg gtgttgccgg
     2341 cgttggagcc gccgacgttg ccgccgccgt aggtgccggt gccgggggcg gtcatgccgg
     2401 tgccgctgcg attgcccatg ttgccggtgt ggccggcgct attggcgccg ccggtgtcca
     2461 tgccggggga acccgtgata cccgcggtcg tcgcgccgtt gcccgcgggg ttgccggctt
     2521 cgtatgcgct atcggtgccg gcgtacgtgc cggagccggt cttcagcgtg tcgcttcgat
     2581 cggtctgctc gccgttgtga ggatagtccg tcatcgaatc acatccgatg acgagcaggc
     2641 ccaaaacagc caaaacagcg gcaaggacga gcgcggaaaa gattctcgaa gtcatgatgt
     2701 tctctccgtt tgttcgaccg cttcgcctgg cgaaatatta cttggccgac tgaccgaccg
     2761 gcggcgacgg cggcggcgcc gcgcgcgtgg gccgcgtcgc cacggcggcg ttgggcacac
     2821 gatcgccctg gcttggtccg atgccggtgc caacctgcgc gtccacgggc atgtcaccat
     2881 tgctgccggt cgcgccttcc accggcgcca ccatcgccgg cttgttgcct ggccgggtcg
     2941 tgctcatcgc gggatcgcgc gagctgagcg aacggccgcc ggcaaactcg ctgctggact
     3001 gcgtcgtcgg cgcggtcgtg ggttgatcat cgaccgccag cgccagcgcg ggcgtcaaca
     3061 agccggtgcc gagcgccagc cgcaggagta tatcgtggat gagcgcggcg gatcgtcgat
     3121 aagttcttct tctagcattc atttgggcct cttttcgctg ttgtaagaga cgcaattgga
     3181 atgccccgag catcaaggaa tatggaaaat tccgcagctc cgacgccggt gtttagacgc
     3241 cgctcaccaa acgcaacggc cacgatggtt gacgacccgc gcccacgcgc gtcgaagagc
     3301 gcgcggagcg caatcaccaa tcaccgtggc cgactgctac gagaaatcca accaccaaaa
     3361 atacggcggc ccgcccgcgg cgcgcctccc gacgcgccgc gagctagttc gttattcgcg
     3421 ctttcgccta cgcctgcgct tccacgttga tcaacgtctc cgccaggctg gtcagcttct
     3481 tatccgcctc gccttcttca tccagcgtct gctgcagcag cttcgccgcc tgcttgttgc
     3541 cgagcaattc ggcatacgcg cgaacggtgc catagccggc aatctcgtaa tgttccacgc
     3601 gttgacattc cgcgatcaac gccgcgtcga gcacctccgg ctcggcgtct tccttcatcg
     3661 tgtccgatgc ctcttcgatg attcccttca tcgctttgca ggtcttgccc ttggccttct
     3721 cgccaaacgc ttcgaacacc tgttccagcc gatcgacctg tcccttggtc acttccagat
     3781 gcctggcgaa cgcatctttc agttcggggg ccgacgcctt tttgatcatc ttcggcagcg
     3841 ccttgatgat ctggttctcc gcgttgtaaa ggtccttgag ctggtcgaca aaaagatcct
     3901 gaagcgattc catttccata acgctctcct tcacttcgaa ggtgatgtag taccgcgaat
     3961 caagccccgc gacaggggcg acgccgcggg gagaccgtgc aatcatcgtg ccccgctcgg
     4021 cggtcgcgga ttttgctcgg tttcgcccgc gggaactttc tgaatcggcg gtggtctaag
     4081 ccatcaattc aacgagcgag gaccacatga cgctgcgacc gcgaccgatc gccctgccga
     4141 tcgctttgtt tctatctgcc ctgctgagcc tgcttctggc gccattcgcg ctggcggatg
     4201 gtttgatcat catccgcaac ggcccggtgg tgcccgggca tttcgccttc gcgccgctcg
     4261 aagtgacata tcaccacgtc gatgtgcgga tcgacgatca ggtggcaacc accagcgtcg
     4321 atgaagagtt ctacaacccc aacggccagc ggctcgaagg cgaatacatc ttcccgctgc
     4381 ccgcggggtc gcacatcgac aacttttcca tggacgtgaa cggtcagatg atgacggccg
     4441 agctgttgcc ggccgacaag gccaagcagt tgtacgagga catcgtccgc cagatgcgcg
     4501 accccgcgtt gctcgaatac accgggcgcg atgcgctgaa ggtgcacatc ttcccgatcg
     4561 agccgaacag ccgaaagcac gtgcagctga agtacacgca gctgctcagc agcgacgcgg
     4621 gcgtcgtcga atattcgtat ccgctgaaca ctgaaaaatt cagcagccgg ccgctgaacg
     4681 atgtgtcggt ccgcgtaacg ctggcgtgca agcagccgat caaatcgatc tacagcccga
     4741 gccacaacgt cgatatctcg cgacatggcg accgcgacgc gacggtcggt tacgaagcca
     4801 aggacgttcg gccggatatg gacttcaggc tgatcttctc gcgcaagcaa acgccgctcg
     4861 gcatcgattt gatgacgtac aagctcggct ccagcgacgg gtacttcatg gtgctcgcct
     4921 cgcccggtgc ggatgatccg gggaagaccg tcacgatccc caaagatgtc tgcttcgtgc
     4981 tcgacaccag cggctcgatg gccgggccga agctcgagca ggcgaagaag gcgatgaact
     5041 tctgcctggc taacctgaac cccgaggacc gtttcgaagt cattcgcttt tcgaccgaac
     5101 cggaagcgct gttcaacggc ctggaaccgg tgaatggtga gaactcgtcg aaggcccgcg
     5161 cgttcgtgga gaatctcaag gccatcggcg gcaccgcgat tgacgaggcg ttgcgcaagg
     5221 cgctcgagct gcgccggccg cagaatgtcg cgcgcgatca atattccgaa gcgatgatca
     5281 aggaagacgg ccggccgttc gtcgtcatct ttctaaccga tggtctgccc acggtcggcg
     5341 agacgcgtga agacccgatc gtcgcgcacg catcgcagga agcgcagtcg cgcaaaggcc
     5401 gcgtcttctg cttcggcatc ggcaacgacg tgaacactca tttgctcgat cgcatcgccg
     5461 gcgacacgcg cgcggccagc cagtacgtcg ggccggaaga ggacatcgaa gtcaaggtca
     5521 gcaactttta ttcgaagatc aaagagcccg cgatgaccga tgtgcagctg gccgtcacag
     5581 gttcgaacgt gcgtacctcg cagctgtatc ccaacgtgat gcccgatctg ttcaagggac
     5641 agatgctcgt cgcgttcggc cgctacagcg gtgacggcgc ggccgcggtc aaggtcagcg
     5701 gcatcgtcaa cggcgcgcac gtcgagttcg cgcaggacgt gacgttcgcc accgagaaca
     5761 tcaacaacgc gttcatcccc cggctgtggg cggcgcgccg cgtcggttgg ttgctggacg
     5821 aaatccgcct gcacggcgag tcgccggagt tgaaggatga ggtcacccgg ctggcgcgcg
     5881 agcacggcat cgtcacgcca tacaccgcgt atctgatcat ggaagacgag cggaagcgca
     5941 gcgtgccgct gtcgctgcag agctatcggg agatgagcac cgacaccgcc gccgcgggcg
     6001 cgggcaaggt atatttcgat tcgatggacg acgaggcgaa gaaggaaagc agccgcagcg
     6061 gccggcaggc cgtcgccaac gccggcaatc tcgcgcagct gaaggacagc cagaacctgt
     6121 cgcagtccac ggtggaagcg ccgatgcaaa agacgcccac cgccggtggc ggtggcttcg
     6181 gctatcgcaa tacgaccaac tacgcccagc aggtccgcgt gatgaacggc cgcgcgttct
     6241 atcagaacgg caacacctgg agcgacggca ccgcgcaatc gcagcagaac ctgaagcaga
     6301 aaaagatcgc gttcaacagc gacgagtatt tcgatctgct caagcaacac cccgaagcgg
     6361 tgtcgtggct gtcgctgggg aatgaagtgg atgtcgtgct ggacgacacg ctgattatgg
     6421 ttcgctaacc aatcgacacg cccattcgcc gaacgtcgcc gcttgcggct tcgcaaacca
     6481 gcccatctgg cctgcgaagc cgcaagcggc atttccggtc acatcccttg ctgtctattt
     6541 gcatttttcc cctctccgcg acaatctaca cctcggtcca caaaggaaag ccgttaccca
     6601 tgatccaccc ctggcacgac gtcacccccg gcgaagatat tccgcaggaa tacaacagcg
     6661 tcatcgaaat ccccttcggc tccagcgtga agtacgaact ggataaatcg agcggcttga
     6721 tcaagctcga tcgcgtcctg tactccgccg tctactaccc cgcgaactac ggcttcgtcc
     6781 cgcagacgct ggccgaggac gatgatccgc tggacgtgct ggtgctgtgc caggaaacgg
     6841 tggtgccgct gacgctgatc cacgcccgca cgatcgggtt gatgacgatg atcgacggcg
     6901 ggaagaagga ccacaagatc atcgcggtgg cgacggaaga tccagaattc aacagctacc
     6961 gcgaagccgc cgagatgccg gcgcatcgcc tgacgatgct gcggcggttt ttccaggact
     7021 ataaacagtt ggaaggcaaa gccgtcgagg tggacgaaat ccaaccggcg aaggtggcgt
     7081 ttccgatcat cgaagacgcg ttggccagat attcgcggca gcgccgcaaa ggcttcggcg
     7141 gcaagtagct ggtcatcccg caggccaagg tgcatacgct tcggccggag cagccgccat
     7201 gccgcatgtt gtggtccttg gcaattcgat cttcgacaac gcgctacgtg cccagcgacg
     7261 agccggtgat cgcgcaggtg cgcgcagcat tgccgcgcgg ccggcaggcg tcgctcctgg
     7321 tcgtcagcgc cggcgggaac gacgggctcg cctccagcta catcctcggc caacgcgcgt
     7381 gtcatccgtc gtcgccgcgc tgaagcatgt gcaggatatc ccccttggct ttagcgatac
     7441 gtatcccaag atgatcggca agctgcggac gacgaacctg ccggccacgg tctgcagggt
     7501 tgacgacgcc gtccccggct tcggcgccgc cgaacttaag tctaaatccg attggtcatg
     7561 gagacgctga aaagggtcgc cccctacacg ccgggttcgg aggtactccg acgacccaga
     7621 tgcctttctc cactcgcgcg aagaatctcg cttcgtcgcc agtacgctcc gaacccggcg
     7681 tgggatgcgt ttcttcaacg cggccgggca tagtgcggtc gacgcgtgaa tttcagtttt
     7741 tgcagcggca aggccgtcaa gagcgccaac ggaagaagta gcacgggcgc gtcttgctgc
     7801 cagacgcccg tgctactcaa ttgtgcgtcc acgcgccgct tcggttaata tcacggcgcg
     7861 cgccgctcgg acgtttcaca catcagactg cggcgcgcgg gacctgttcg ggaggattcg
     7921 ggcgtgagac atcgaaaagt gatcgcggcg ctggccaccc tgccggtgct cgggcttgtc
     7981 gggggactgg gttattacgc ggtgaagaat cggcagattg aggccgatct gaacgccgcc
     8041 gtcgcgcgcg ccgaggcgtc caaaccggtg caggtggact tcgccgtgtc ggtgccgggc
     8101 gacacgccca aggatcagat catctacttg tccggcagcg taccggcgct gggcaactgg
     8161 gacgcggcgg gcgtgccgat gcagcggcag gacgatggca agtggcacgc gaaggtcacg
     8221 gtgctgagcg ggatcgacta cggctacaag gtcacccgcg gcacgtgggg caccgtcgaa
     8281 accgacgcca agggcgcagc gatcgccgat cattcgctga acgttgaagg cccgcgcgcg
     8341 atggacgtct cggtggccga atggatcgat cacggccagg ccgtgcccgg ccgcgtgacg
     8401 atgaccggcg acttgcgcct cacaaaaaat ttcccctcga agctgctgaa caacacccgc
     8461 acgctgatcg tctacctgcc gccggggtat gaccagaaca ccgccgatcg ctatcccgtg
     8521 ctgtacatgc aggacggcca gaatttgttc gacgaagcga cgagctttgc gggcgtcgag
     8581 tggaagatgg acgaggcggc ccaggcgctg atcggccagg gtgaagcttc gccgttcatc
     8641 gtcgtcggca tctataacac gccacagcgc accggcgaat ttacgccccc aaccatggcc
     8701 gacgccgctg gcagcgccag cgatcacacg cccgcgcatg gcgatctgta cgcccggttc
     8761 gtcatcgagg aagtcaagcc gttcatcgac cagacgtatc gcacgcagac cgatcgcgcg
     8821 cacacgctca tcggcggcgc agccctgggc gggctcgaag ccctctacac ggcgcagtcg
     8881 cacagtgaca tcttcggcca ggtcatcgcg ctctccccct ggcttcgcca gaacgacaag
     8941 aagctggtcc cgcagttcgt cggcgacggc aagtggctgc acagcgaacg cctctacgcc
     9001 agcatgggca ccgaccccgg tgacaacggc aacaactacc ccggcggccc caccgccgcc
     9061 gtcgccgacg gcgccgagct ggcgcaggcg ctgaagtccg ccggacttca gccggacaag
     9121 gatttcgtct accgcgagat caaaggcggc caccaggacg aacccagttg gcaagccgaa
     9181 atcggcgacg tgctgaagtg ggtcttcgcg cgaccggcaa cggaatcaac gaccacgcca
     9241 gcggtcgagt aaatcggcgc aatcgagcac acgttatcga ctgccgcaga acggagaatg
     9301 gaagatcatc acgctcgggt aagagccgcg cacggagcgt actcgcgcag taagcggctc
     9361 ctcatgacct tcattccgca ttcccgcatt cggcgttcat cattctgcat tcgcgcccca
     9421 ctccccgctt tcgcttcatc ggcgcacgcg gtatcgttcg ccaccggttg atgcgaattt
     9481 gctgacatca ccgatcaacc agagcgcacc gcggtaacca tggcctgggc gtactacctt
     9541 ctcctgatcg cctccgacat cgtcgggctg ttgctcgcgg cgttcacgct gccagggctt
     9601 tggttgatgc tcgccgccgc cgcggggtat gcgtttctca cgcacgggca gtacctcagc
     9661 tataagacac tcatcgcgct tttggtgctg gcggcgattg cggagatcgc ggagttcgtc
     9721 ctcggtggcg ccggcgccaa aaaagccggc gcgagcaaat ggggcatcgt cggcggtctc
     9781 gtcggcgcga tcctcggcgg cttgttcctc accggtgtca tcccgatctt cgccccgctc
     9841 agcacgatca tcggcatctg cctcggcagc ttcgccggcg cgttcggcat cgaactcgcg
     9901 ctcggccggc cgatcatgca gtcggtcaac atcggttacg gcgccgccaa gggccggttg
     9961 ctcggcatcg ccaacaaggt tgccgtcggt gtggtgatgt tcttgatcac actctgggtc
    10021 gcgctgccga tccatggcac gcaaccaccc ccggcgacgg cgtcgccgcc cgtcaacggc
    10081 gcagcgccaa cgacggcgac gaccacccca tcgccgtgaa gaagcgcaat tcccccgcgt
    10141 agcggtagcc ccgaaggggc acctgttttc gttcgagtcg gaaaacacgt ccccttcggc
    10201 gccaccgcca cgcaacacat cctccgaatc tttttacctt tcgatgcagt tagggtatcg
    10261 ttcacccacc aaacaggggc cgctgcatgt ccgttcgatt catccttggc cgcgccggcg
    10321 ccggcaaaac acgatactgc ctcgagcaga tcgaacagct cgtgcgcgct gagccgctcg
    10381 ggccgccgat cttctggctg ctgccgcgcc aagccacgtt tcaag
//
LOCUS       DATIMH010000681         7455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3339577, whole genome shotgun sequence.
ACCESSION   DATIMH010000681 DATIMH010000000
VERSION     DATIMH010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3339577"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1441)
                     /locus_tag="VLI90_11900"
     CDS             complement(128..1441)
                     /locus_tag="VLI90_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14952.1"
                     /translation="MQAVIENRTSALIADRDDRALDRRIAVALFIIATVIATLFVQIR
                     GEFEKLGEEPRVGIHVSLGHGFRTPLDLSPDAEISAWMPPIYPIVSGTAFRLFGGPGP
                     LAFRLLMEINAICYGLMVAGTFVMARLLLSRPAGVLAAVFVICHPMFLMLVHRFWHTY
                     LAQAGMIWLIVAALRTQRSGPTFLRLALIGAGLGILVQMNGSFILVAPIIVWLAVRDL
                     PIARWFPFMAASAITFILMLVPWTVRNYVQFHQLTYVRRGAELEMWLGNLPGSNGWQD
                     LRFHPTVYLPEHQKMLEMGEANYFAYCKERFLENYHRAPMEYWKRCARRALYLVIGEP
                     NSRPMEWKHASAWFGQLRLIMDVLIFSLGTAGLIAAWRLGYRTAWVVPFSIASTVPYL
                     ISHMNYRFSMHIKLLLLMMIAFLIWSIWQRRVMGAWPRRDVVVNA"
     gene            complement(1677..2720)
                     /locus_tag="VLI90_11905"
     CDS             complement(1677..2720)
                     /locus_tag="VLI90_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSV14953.1"
                     /translation="MDDTISPSQTKSAGQTGLSYKFQRLREKLRQAIASGELSGKLPG
                     ERALAKRFHVNAKTLSKALTDLAAEGVLDRSIGRGTYVKGFAPATIAAKRWLVVCDPE
                     QVGWELVQMIRAAHPEMDVATDVTAVRPSFLNQFSAVIDLSSNTPDAFLRDLVVRNIP
                     VVVVGKEPKTYSTHAVLFDGPLAVSQIGRDLMLGGHRHLAAVEPRQCTVVADTLRKVA
                     ARYAPKAHIDACFAADVPAMVESGVTAFVCQSIDLARAVKDQLDKRDIAIPGRVSLAA
                     VGTTNDDQPVSGYFLHRSEKANAIVQLLSDTQSYRPTTLWLAGRFVDRGTISPLTAMG
                     EEETPMRYGVVSA"
     gene            2943..3938
                     /locus_tag="VLI90_11910"
     CDS             2943..3938
                     /locus_tag="VLI90_11910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSV14954.1"
                     /translation="MLEWSDDLKIFDCSLYLDSCTPRGLCFISHAHSDHIGIHNSVIA
                     TAATAALTEHRLGPQSVTELEFGAHHSIDAATEVSLLSAGHILGSAMLYVRRSGGSLL
                     YTGDFKLRSSLTVPPATPVKADVLVMESTYGQPHFRFPPWQEVAAQLVELVSDALRAG
                     RQPIVMGYSLGKAQEIVRILTDAGITVTCHGAIVSMNGIYERFGVRVGAVRRYRVEDF
                     HGTSALDLQERGVLVAPPQVARTALVTRFDNPCRIMMTGWSLLKNAIYRYGVDHALPL
                     SDHADFDELMELIERVEPRKIYTHHGYREFVNILKSRGHNAELARPDEQLLLFPE"
     gene            4081..4599
                     /locus_tag="VLI90_11915"
     CDS             4081..4599
                     /locus_tag="VLI90_11915"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HSV14955.1"
                     /translation="MATIEVIEGDITTQHVDAIVNAANESLMGGGGVDGAIHDAAGPE
                     LLEQCKAVAPCPTGEARLTKAYNLPAKYVIHTVGPVWNGGGHGEDQLLASCYRHSLSL
                     ALRHDVRSIAFPAISTGAFGFPVERAATIAADETERFLRTEGRPEKVYFVCFDRATYD
                     AYSRAVRALDER"
     gene            complement(4673..4861)
                     /locus_tag="VLI90_11920"
     CDS             complement(4673..4861)
                     /locus_tag="VLI90_11920"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HSV14956.1"
                     /translation="MQGPLRRSTSNQMIAGVCAGIADWLGWDPTVVRILFVAATLFTA
                     AFPGTLLYIIIWVVMPVL"
     gene            complement(5047..5283)
                     /locus_tag="VLI90_11925"
     CDS             complement(5047..5283)
                     /locus_tag="VLI90_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14957.1"
                     /translation="MIFADTGYYIALFDRNDQLFLVAAAWSRTIAEPMLVSEHVLLEV
                     VNYFSRRPRRPRAHAVVDAIVADPDTIVAPASRE"
     gene            complement(5280..5525)
                     /locus_tag="VLI90_11930"
     CDS             complement(5280..5525)
                     /locus_tag="VLI90_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14958.1"
                     /translation="MTVTLRAAFDAKVLVPIGPVDLPVGQELEIQVVEQPAVNGKSGL
                     QRLLEIAEQFPPDAEAPTDGAAQHDHYLYGTPKREDP"
     gene            complement(5556..7028)
                     /gene="gatB"
                     /locus_tag="VLI90_11935"
     CDS             complement(5556..7028)
                     /gene="gatB"
                     /locus_tag="VLI90_11935"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HSV14959.1"
                     /translation="MPERIVKSYKVLVGMEIHVQLATQSKMFTGAANGFGGEPNAQVD
                     TVVLGLPGVLPVMNKRAVEYAIKVGLALGCRIAKHTKWDRKSYYYPDLPKNYQISQYD
                     LPLCAEGEFQLTLKDGSERKVRIRRAHLEEDAGKLLHEAPGGYHIDWSIVDLNRAGTP
                     LLEIVTEPDLSSGEEVATFGQELQKLVQFLGVSHGQMQMGHMRFEPNINVHITDQDGN
                     VHKTAITEIKNLNSFSVLQRATDYEVQRQMRQWVETGSLGKKSTYGWDEAGEATVFQR
                     FKEGESDYRYFPDPDLVPVEVGEQWLTELKAEVGEMPVARRKRYVDQFGLSPADAATL
                     AGDRKLGDFYEAALASNAKPSRTANLILSHGFRLSNSKSKPLYELGLSPGAFGEVATM
                     IDADQVAASAAGPIFDRLAETGGTSAAAVADELGLRQVSDTGPIDAAIDAMIASNPKA
                     LQEFKAGKQSAMGALMGIVMKSGKGLNPKLVQERLKAKLQ"
     gene            complement(7096..>7455)
                     /locus_tag="VLI90_11940"
     CDS             complement(7096..>7455)
                     /locus_tag="VLI90_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HSV14960.1"
                     /translation="NFRNKLVLALDAKDGYVATRGWTETSKKLATDVAKQVSGWPLAA
                     ILYTDVAKDGMMTGPNFEATAKLAETGDVPVIASGGVGSIEHIRILRELPVWGVIVGR
                     SLYEGKLDLKEAMSLAK"
BASE COUNT         1372 a         2278 c         2392 g         1413 t
ORIGIN      
        1 gcgaagccgc aagcggctga tttgcgcaaa cgacgaatct ccgacttcgc ggctagatcc
       61 ttcgactgcg cacgagtacg tgctctgctc tggatgacgc gcgcaccccc cgcgggcttc
      121 aacttattca agcattcacc acaacatcgc gccgcggcca cgctcccatg acgcgccgtt
      181 gccagatcga ccagatcagg aacgcgatca tcatcagcag cagcagcttg atgtgcatcg
      241 agaatcgata gttcatgtgg ctgatcaggt acggcaccgt gctggcgatc gagaacggca
      301 ccacccacgc ggtgcggtag ccgagccgcc aggcggcaat gagacctgcc gtgccgaggg
      361 agaagatcag cacgtccatg atgaggcgga gctggccgaa ccacgcgctg gcgtgtttcc
      421 attccatcgg gcggctgttg ggttctccga tgaccaggta cagcgcccgg cgggcgcagc
      481 gcttccagta ttccatcggg gcgcgatggt agttttccag gaagcgctct ttgcagtagg
      541 cgaaataatt cgcctcgccc atctccagca tcttttgatg ctcggggagg taaacggtcg
      601 ggtggaagcg aaggtcctgc cagccgttcg aaccggggag gttgccgagc cacatctcca
      661 gctccgcccc gcggcggacg tacgtgagtt ggtggaactg cacgtaattt cgcaccgtcc
      721 acggcacgag catgaggatg aacgtgatgg cgctggccgc catgaatggg aaccagcgcg
      781 cgatcggcag gtcgcgcacg gcaagccaga cgatgatcgg cgcgaccagg atgaacgaac
      841 cattcatctg aacgaggatt cccagtccgg cgccgatgag cgcgaggcga aggaacgttg
      901 gaccggagcg ctgcgtgcgc agcgcggcga cgatcagcca gatcatcccc gcctgcgcca
      961 ggtacgtgtg ccagaaccgg tgcaccagca tgaggaacat cggatggcag atgacgaaga
     1021 cggccgcgag gacgccggcg ggccggctca gtaagagccg ggccatcacg aacgtgccgg
     1081 cgaccatcag gccgtagcag attgcattga tctccatcag cagtcgaaac gccagcgggc
     1141 cgggcccgcc gaacaggcgg aacgcggtgc cggagacgat cgggtaaatc ggtggcatcc
     1201 acgcgctgat ctcggcgtcg ggcgacagat cgagcggcgt tcgaaacccg tgaccgaggc
     1261 tgacgtgaat gcccacccgc ggttcttcgc cgagcttttc gaattcgccg cggatctgca
     1321 cgaacagtgt ggcgatgacg gtggcgatga tgaagagcgc gaccgcgatg cggcggtcga
     1381 gggcgcggtc atcgcgatcg gcgatcagcg cggacgttcg gttttcgatt acagcttgca
     1441 caaggactcc aaagcgtcga ttcgagcggc gcatcagcgc gcgggacgcc cccggcgcaa
     1501 tatcggcaaa aatgttaggg gacttgggga gaacaaaacc acgccgggtt cggaggcctg
     1561 ccctgagcga agtcgaagga tactccgacg acccggattc tttcctgcga ttgggcagaa
     1621 gaatccgggt cgtcgcgaac acgctccgaa cccggcgtgg gggtggtttt gaagtttcag
     1681 gcagagacaa cgccgtagcg catcggggtt tcttcttcgc ccatcgctgt cagcggggag
     1741 atcgtgccgc ggtctacgaa gcggccggca agccagagcg tcgtcgggcg gtagctctgc
     1801 gtgtcactta gcagctgcac gatggcgttg gctttttccg agcggtgcag gaaatagcca
     1861 ctcaccggtt ggtcgtcgtt ggtcgtgccg acggcggcca gcgagacacg gccggggatg
     1921 gcgatgtcgc gcttgtcgag ttgatctttc actgcgcggg cgagatcgat cgattggcag
     1981 acgaacgcgg tcacgccgct ttcgaccatc gcgggcacgt cggcggcgaa gcaggcgtcg
     2041 atgtgcgcct tgggcgcata acgcgcggcg acctttcgca gcgtatcggc gacgaccgtg
     2101 cattgccgcg gttcgacggc agccagatgc cggtgcccgc cgagcatcag atcgcggccg
     2161 atttgtgaaa cagccagcgg cccgtcgaac agcaccgcgt gcgtcgagta cgtcttaggt
     2221 tccttgccga cgacgaccac cggaatgttc cgcacgacca ggtcgcgcag gaacgcgtcg
     2281 ggcgtgttcg acgacaggtc gatcacggcg ctgaattgat ttaagaagct gggacggacg
     2341 gcagtgacat cggtggcgac atccatctcg ggatgagcgg cgcggatcat ctgcaccagt
     2401 tcccaaccca cctgctcggg gtcgcacacg acgagccagc gcttggcggc gatcgtcgcg
     2461 ggggcgaagc ctttgacgta ggtcccgcgg ccgatgctgc gatcgagcac accctcggcg
     2521 gccaagtcag tcagggcttt gctcagcgtc ttggcgttca cgtggaaacg tttggccaag
     2581 gctcgctcgc ccggcaattt gccggacagc tcgccgctgg cgatggcctg gcgcagcttt
     2641 tcgcgcaagc gctggaactt atagctgagc cccgtctggc cagcgctttt ggtttgtgac
     2701 ggcgagattg tgtcatccat gagcgaatcc ttgctgagaa ccagacggta acagacgcaa
     2761 cgcaatgcgg accgcatcga cttcggcgaa ccggtcagcg ccgttgatcg gtctaatacg
     2821 gcacgttgac ttgcacaaat gtcgccaatc aggtccccac cggttgtgta gaacatctaa
     2881 catatcttcc ggcaaagcgt caaaccgccg cacaaccatt ttgccgaggc gtatgattgt
     2941 aaatgcttga gtggtcggat gatctgaaaa ttttcgattg ctcgctgtac ctcgatagct
     3001 gcacgccgcg gggcctgtgc tttatctccc atgcgcattc ggatcacatc ggaatccaca
     3061 attccgtcat cgccacggcg gcaacggcgg cgctgaccga gcaccgcttg ggcccgcaaa
     3121 gtgtgaccga gctggagttc ggcgcgcatc actctattga cgccgcgacg gaggtttcgt
     3181 tgctgagcgc cggccacatt ctgggcagtg cgatgctgta cgttcggcgg agcggagggt
     3241 cattgctata taccggcgac ttcaaattga ggtcgtcgct gacggtcccg ccggccacgc
     3301 cggtgaaggc ggacgtgctg gtgatggaga gcacatatgg acagccgcac tttcgcttcc
     3361 cgccgtggca agaggtggcg gcccagctgg tggagctggt gagtgacgcc ctacgcgccg
     3421 ggcgtcaacc gatcgtgatg ggctacagcc tgggcaaggc gcaggagatc gttcgcatcc
     3481 tcaccgacgc ggggatcacc gtgacgtgtc acggcgcgat cgtctcgatg aacggcatct
     3541 acgagcgctt cggcgtgcgc gtcggcgcgg tccgccgata tcgcgtcgaa gattttcatg
     3601 gaaccagcgc gctcgatctg caggagcgtg gcgtgctggt cgccccgccg caggtggcgc
     3661 ggacggcgtt agtcacgcgg ttcgacaacc cgtgccgcat catgatgacg ggctggtcgc
     3721 tgctgaagaa cgcgatctat cgctatggcg tcgatcacgc gctgccgctg agcgatcacg
     3781 cggattttga cgagttgatg gaactgatcg aacgggtgga gccgcggaag atttacacgc
     3841 accatgggta ccgggagttc gtcaatatct tgaagagccg cgggcacaat gcggagttgg
     3901 cgcgaccgga tgagcaattg ctgttgtttc cggaatgact actcgacggc gtgctgctct
     3961 gttgctgcga cgcctgcgtc gcctttgcgc gagagggcga cgcaggcgtc gcagctacaa
     4021 aagatccgaa aggaatgggg ttattcggcg cgcgatgccg cggtatgatg cgcaacgcac
     4081 atggccacca tcgaggtcat cgaaggcgac atcaccactc agcacgtgga cgcgatcgtc
     4141 aacgcggcga acgaatcact catgggcggt ggcggcgtgg acggcgcgat tcacgacgcc
     4201 gctgggccag agcttttgga acaatgcaag gccgtagcgc cctgtcccac gggtgaagcg
     4261 cgtctgacca aggcgtacaa cctgccggcg aaatacgtca tccacacggt cggcccggtg
     4321 tggaacggcg gcggccatgg cgaagatcag ctgctggcca gctgctatcg ccacagcttg
     4381 tccttggcgc tgcggcatga cgtacgctcg atcgcgttcc ccgcgatcag caccggcgcg
     4441 ttcgggtttc ccgtagaacg agccgcgacc atcgcggcgg atgagacgga gcgatttcta
     4501 agaaccgaag ggcgaccgga gaaggtgtat ttcgtctgct tcgatcgcgc gacgtacgac
     4561 gcgtacagcc gcgcggtgcg ggcgctggat gagcgatagc gcgaaacgtg gtgcgtttag
     4621 ccgccgacgc ctcgcggcgc ggatgtacga aaacgccgca gggccgcggc ggctaaagga
     4681 ccggcatcac cacccatatg atgatgtaca gcaatgtccc cggaaatgcc gcggtgaata
     4741 gtgtggcggc aacgaacagg atccgcacga ccgtcgggtc ccagccgagc cagtcggcga
     4801 tgccggcgca cacgccggcg atcatctggt tgctggtcga tcggcgaagc ggtccctgca
     4861 tagcattcat cctcggcggt cacggatcgc gccgcaagag ggcttcgaaa ccggcttgct
     4921 cgaagtggtg gtcgaacgcc agcgctctgg tgagacgcct gcgccgcatc aactcgaacg
     4981 aaatgcaatc ggtgagggac caatccttgt ccactgaccg caatgcaaat ccagtgcggc
     5041 cttgaatcat tctcgtgagg cgggcgcgac gatcgtgtcc gggtcggcga cgatcgcatc
     5101 caccacagca tgtgcgcgcg ggcggcgcgg ccgtctggaa aagtagttca cgacctcgag
     5161 gagaacgtgt tcactaacaa gcatgggttc agcgatcgtt ctcgaccaag cagctgccac
     5221 gaggaaaagc tggtcgttgc ggtcgaacag cgcgatgtag tatccggtgt cggcgaagat
     5281 cacggatctt cccgcttggg ggttccgtac aggtagtggt catgctgcgc cgccccgtcg
     5341 gtgggcgctt cagcatctgg cgggaactgt tcagcgattt ccagcaaccg ctgaaggcca
     5401 cttttcccgt tgaccgccgg ctgctccacg acttggattt ccagctcctg ccccaccggt
     5461 agatcgaccg ggccaattgg caccagcacc tttgcgtcga acgcggctcg aagcgttacc
     5521 gtcatagctt cattgtagcg attctttagg catcgctact gcaatttcgc cttcaaccgc
     5581 tcctgcacca gcttcgggtt caggcccttg ccgctcttca tgacgatgcc catcagcgcg
     5641 cccatcgcgc tttgtttccc ggctttgaat tcctgaagcg ctttgggatt gctggcgatc
     5701 atcgcgtcaa tggcggcgtc gatcgggccg gtgtcgctga cttgccgcag gcccagttcg
     5761 tcggcgacgg cggcggcgct ggtgccgcca gtctcggcga ggcgatcgaa gatcggcccg
     5821 gcggcgctgg cggcgacctg gtcggcgtcg atcatcgttg ccacttcgcc aaacgcgccg
     5881 ggcgacaggc ccagttcgta cagcggtttg gacttgctgt tcgacaggcg gaagccgtgg
     5941 ctgaggatca ggttggcggt gcgcgacggc ttggcgttgc tggcgagcgc ggcttcgtaa
     6001 aaatcaccga gtttgcgatc gccggcgagc gtcgcggcgt cggccggtga taagccgaat
     6061 tgatcgacgt aacgtttgcg ccgcgcaacc ggcatctcgc cgacttcggc tttcagctcg
     6121 gtcagccact gctcgccaac ttcgaccggc accaggtcgg ggtcggggaa gtagcggtaa
     6181 tcgctctcgc cttccttgaa gcgctggaac acggtcgcct cgccagcctc gtcccagccg
     6241 taggtgctct tcttgccgag cgaacccgtt tccacccatt ggcgcatctg ccgctgcact
     6301 tcgtagtcgg ttgcgcgctg cagcacgctg aagctgttca gattcttaat ctcggtgatc
     6361 gccgtcttgt gcacgttccc gtcttgatcg gtgatatgca cgttgatgtt cggctcgaag
     6421 cgcatgtggc ccatctgcat ctgcccgtgg ctgacgccga gaaactgcac gagtttctgc
     6481 agctcctgcc cgaacgtcgc gacctcttca ccactggaca aatccggctc agtcacgatc
     6541 tccagcagcg gcgtgccagc gcgattgaga tcgacaatcg accaatcgat gtggtagccg
     6601 ccgggggctt cgtgcagcag cttgccggcg tcttcttcga ggtgcgcccg gcgaatccgc
     6661 accttccgct cgctgccatc cttcagcgtc agctggaatt cgccctccgc gcacagcggc
     6721 aaatcgtact ggctgatctg ataattcttg ggcaggtcag ggtaataata acttttgcga
     6781 tcccacttgg tgtgcttggc gatccggcag cccaaggcga gtcccacctt gatcgcgtac
     6841 tcgacggccc tcttgttcat gaccggcagc acgccgggca ggccgaggac gacggtgtcc
     6901 acctgcgcgt tcggctcgcc gccgaacccg ttggcggcgc cggtgaacat cttcgattgc
     6961 gtggccagct gcacgtggat ttccatgccg accaggactt tgtacgactt gacgatgcgt
     7021 tcaggcacgg atgttctcct gtagcacggg cgtcccgccc gtgtcatgcg gctcggcacg
     7081 ggcgagacgc ccgtgctact tagcaagact catcgcttcc ttcaaatcca acttcccttc
     7141 gtacaaactg cgcccgacga tcacgcccca caccggcagc tcgcgcaata tccgaatgtg
     7201 ttcgatgctc cccacgccgc cgctggcgat caccggcacg tcgccggttt cggcgagctt
     7261 cgcggtcgcc tcgaagttcg ggccggtcat catgccatct ttggcgacgt cggtgtaaag
     7321 gatcgccgcc agcggccagc cgctcacctg cttcgcgacg tcggtcgcca gtttctttga
     7381 agtctcggtc cacccgcgcg tggcgacgta gccgtctttc gcatcgagcg ccagcaccag
     7441 cttgttgcga aagtt
//
LOCUS       DATIMH010000682         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2762156, whole genome shotgun sequence.
ACCESSION   DATIMH010000682 DATIMH010000000
VERSION     DATIMH010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762156"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VLI90_11945"
     CDS             <1..844
                     /locus_tag="VLI90_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14961.1"
                     /translation="GAAGMALYATSPSEVVGGESLRLLGQTLGFGLPLLLLALLFVRL
                     NLTAPAVGVTRVITVAIFVVGTLVLPVYSNVRARTDEEHRRRNAEWWANQQARIEQGK
                     AALAALPADAPLEKIIPYLVEDWPAEVRGDAFTKLQHRPPVYDELRPMLSGANRDLAL
                     AYMTQVSDAPPMDLAATVRDRLIDVANQWRDRLRAGALNDEQINTFGRQCELASSLSF
                     KFRECGVDFRPVADVWHAAVDAAPQQLPIVQGAQSTLLFWRDRNDRDSAPPAAATPPL
                     QPAG"
     gene            890..1414
                     /locus_tag="VLI90_11950"
     CDS             890..1414
                     /locus_tag="VLI90_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14962.1"
                     /translation="MVPVLLETPASNSVWIIIAVCGATILYAIFRPSARSKRDPLTRV
                     PSSARLSQQRNTERQMESLLVELSEMARQISAQLDTRSQKLELLIQEADAKIAEMERA
                     RAGATGPTSSDHQTWSTHSEPPPAETDVIDPRHQEIYTLADQGMSAPDIARQLDRPRG
                     EVELILALRPRTRT"
     gene            1639..3075
                     /locus_tag="VLI90_11955"
     CDS             1639..3075
                     /locus_tag="VLI90_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSV14963.1"
                     /translation="MDIFEKILATLALVGTNGYFVAAEFAAVGARATRLQIEAEHHFL
                     SRLALLVKQKLDLYLSSCQLGVTLASLGLGAVTEPAVGAVLRPVLQTLRVPQRDEYTI
                     SFIAALAISTSLHIVIGEQAPKNWAIRYANRLLPPLALPLVIFTFLFYPAIWLLNVAT
                     NAVLRATGMKSGLGQGEGHLPHNERELRALLAQAVAAGTIARGNERILTSAFEFGDLK
                     VRQIMTPRTKVDYLTLDQPIGEVLRTVQKSAYTRLPLCDGDIDHVIGLIHMKDLFNHL
                     QLVPGKLRFVDERAPGGEMIAIPDGLPGSAVHVIGAGDIDLRKIKREVLFVPELLPVP
                     RMLRQFQTKQIHMAVVVDEYGATLGIATLEDVIEEIVGEIEDEFDTAGPKPFVKEGES
                     FRVAGTYPLHELRERLVFPADLEVGDVDTIGGYVIAELKRWPRVGDTIEMGNYSVRVM
                     AVQGNRVQQVFITPVTQEAARQDGPGQA"
BASE COUNT          598 a         1024 c         1155 g          559 t
ORIGIN      
        1 cggggcagcg gggatggcgc tgtacgcaac ctcgccgagt gaggtcgttg gcggcgaatc
       61 gctgcgcctg ctcgggcaga cgctgggctt cggcctgccg ctgctgctgc ttgcgctgct
      121 gttcgtgcgg ttgaacctca cagccccggc ggtcggcgtc acgcgcgtca tcaccgtggc
      181 gatcttcgtg gtcggcacgc tcgtgctgcc ggtgtattcg aacgtgcgcg cccggaccga
      241 cgaggagcat cgccgtcgaa acgcggagtg gtgggcgaat cagcaggcgc ggattgagca
      301 gggcaaggcc gcactggcgg cactgccggc cgatgcgccg ctggagaaga tcattcctta
      361 cctggtcgag gactggccgg ccgaagtgcg cggcgatgct tttacgaagc tgcagcatcg
      421 cccgccggtg tatgacgagc ttcggccgat gttgagcggg gcgaaccgcg atctggcgct
      481 ggcgtacatg acgcaggtct ccgacgcgcc gccgatggat ctggcggcga ccgtgcgcga
      541 tcgcctgatc gacgtcgcca atcaatggcg cgatcggctt cgcgccggcg cgttgaacga
      601 cgagcagata aatacgtttg gccgccagtg cgaactggcg tcgagcctgt cgttcaagtt
      661 ccgcgagtgc ggcgtggact ttcgtccggt cgccgacgtc tggcatgccg ccgttgacgc
      721 ggcgccgcag cagttgccga tcgtgcaggg cgcgcagagc acgctgctct tttggcgcga
      781 ccgtaacgat cgcgacagcg cgccacccgc ggccgcgaca ccgccattgc aacccgccgg
      841 gtgatgcgtc aggatacgcc gcacagggat ggagcccaca gggttgaaga tggttcccgt
      901 tctgctcgaa acgcccgcca gcaacagcgt gtggatcata atcgccgtct gcggcgcgac
      961 gatcctctac gcgatcttcc ggccatcagc ccgcagcaag cgcgacccgc tgacgcgcgt
     1021 cccttccagc gcccgactct cgcaacaacg caacaccgaa cggcaaatgg aaagcctgct
     1081 ggtcgagctt tcggaaatgg cgaggcagat cagcgcgcag ctggacacaa gatcgcagaa
     1141 gctcgagcta ttgatccagg aagccgacgc gaagatcgcg gagatggaac gcgcccgcgc
     1201 cggcgctacg ggtcctacga gcagcgatca ccaaacttgg tccacacaca gcgagccgcc
     1261 gccggcggag acggacgtga tcgacccgcg gcaccaggag atctacacgc tggccgacca
     1321 aggcatgtcg gcgccggaca tcgcgaggca gttggatcga ccgcgcgggg aggtcgaact
     1381 gatcctcgca ttacgtccgc gcacgcggac ctgaacgcac gtttcgctcg cgcaacagcc
     1441 ccatttagcc cgacgtgaac acacgccggg tttacgtaga tcatcaggga ctgccgattg
     1501 agaaaacaaa ctcgcctcga cttctcagcg tagcccggcg tgtgttcacg ccgggctaaa
     1561 tggggggtgt ctactgctgg cgcggcgttg cgcggcgttg gttgtcacca agtggccagg
     1621 ggggtacaac gccacgcggt ggatatcttc gagaaaatcc tggccacgct cgcgctggtg
     1681 ggcaccaacg gctacttcgt cgccgcggaa ttcgcggcgg tgggagcgcg cgccacgcgg
     1741 ctgcagatcg aggcggaaca tcacttcctc tcgcggctgg cgctgctggt gaagcagaag
     1801 ctggacctgt atctgtcgag ctgccaactc ggcgtgacgc tggcaagcct ggggctgggg
     1861 gccgtgactg agcccgcggt gggggcggtg ctgcggccgg tgttgcagac gctgcgcgtg
     1921 ccgcagcgcg atgaatacac gatcagcttc atcgccgccc tggcgatcag cacgtcgctg
     1981 cacatcgtga tcggcgagca ggccccgaag aactgggcga ttcgttatgc caaccgcctg
     2041 ctgccgccgt tggcgttgcc gctggtcatc ttcacgttcc tgttttaccc ggccatctgg
     2101 ctgttgaacg tggcgaccaa cgcggtgctg cgcgcgaccg gcatgaagag cgggctgggg
     2161 cagggcgaag gccacctgcc gcacaacgag cgcgagctgc gcgcgctgct ggcgcaggca
     2221 gtggcggccg gcaccatcgc gcgcgggaac gagcggatac tgaccagcgc gtttgagttc
     2281 ggcgatctga aggtccgcca gatcatgacg ccgcggacga aggtggatta cctcacgctc
     2341 gatcagccga tcggcgaggt gttgcgcacg gtgcagaaga gcgcgtacac gcggctgccg
     2401 ttgtgcgacg gcgacatcga ccacgtgatc gggctgatcc acatgaagga cctgttcaac
     2461 cacctgcagc tggtgccggg caagctgcgc ttcgtcgacg agcgcgcccc cggcggcgaa
     2521 atgatcgcga tcccggacgg gctgccgggg tcggcggtgc acgtcatcgg cgctggcgat
     2581 atcgacctgc gcaagatcaa gcgcgaggtg ctgttcgtgc cggaattgct gccggtgccg
     2641 cggatgcttc ggcagtttca gacgaagcag atccacatgg ccgtcgtcgt ggacgaatac
     2701 ggcgcgacgc tcggcatcgc cacgctggaa gacgtcatcg aggaaatcgt tggcgagatc
     2761 gaagatgaat tcgacaccgc ggggccgaag ccgtttgtga aggaagggga aagttttcgc
     2821 gtcgccggga cgtacccgct gcacgaactt cgcgagcggc tggtgtttcc cgcagatttg
     2881 gaagtcggcg acgtcgatac gatcggcggg tatgtgatcg cggaactgaa gcgctggccg
     2941 cgcgtgggtg acacgatcga gatgggcaat tattcggtgc gcgtgatggc cgtccagggc
     3001 aaccgcgtgc agcaggtgtt catcacaccg gtgacgcagg aagccgcccg ccaggatggc
     3061 ccggggcagg cgtgacggtt tgttctgaaa tgcaaccgcc gaggagccga gagcgccgag
     3121 gcggacgccg agaagacgtt gcgactgtca tcctgagcgg agcacgtact cgtgcgtagt
     3181 cgaaggatct ggccgggaag tatttgaatg gtgcatccgt gcgcggggag ccggcatgta
     3241 aatgcggggc ttcgaacgca gatgaacgcc tgcgctaacg gatcgctcca tcacttctca
     3301 tcaagatcct tcggctccgg cgagtacgcc tgcgct
//
LOCUS       DATIMH010000683         1825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5366456, whole genome shotgun sequence.
ACCESSION   DATIMH010000683 DATIMH010000000
VERSION     DATIMH010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5366456"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..1282
                     /locus_tag="VLI90_11960"
     CDS             476..1282
                     /locus_tag="VLI90_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HSV14964.1"
                     /translation="MRDSVSWVHHFEPYTATHACVVAAFVMLVAAMVAMRRRDDIATV
                     PPNRRAMDYAIGYIGLAAAVFVQVVTLWPGRFDVWTSLPLHVCDICMFVAPLSLLLGW
                     RPLRAIAYFWGLGLSSMSFIFPDLRFGPGDFQFWVFWTGHATIVGTALYDITARGYRP
                     RFGDWWIAVKFGVFYAGTIFLVDALWHFNYGYVGQTYKNQRSPADFLGPWPWRVPLMV
                     AMAFAAMFLLLAPWLLWRRHGGTGYQSAIRGMPRMSALFHRRSDRASEGV"
     gene            complement(1371..>1825)
                     /locus_tag="VLI90_11965"
     CDS             complement(1371..>1825)
                     /locus_tag="VLI90_11965"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSV14965.1"
                     /translation="AATSSQLKSAFEMHAQQTQQHVARLERVFRSINCQPESESCPAM
                     RGLIKEGQEMIDAQGDCSVKDAGLIAAAQRVEHYEMAAYGTVRSLAQQLGLSDAAQIL
                     QQTLDEEGETDKRLTQIAESQANAMSPQGMPQGAGSTQQWNGSTQYHA"
BASE COUNT          259 a          585 c          568 g          413 t
ORIGIN      
        1 ttcttcgcgg agtcgagttt tttcttccga aaccggcgcg tttggagaat ttaggtcgtt
       61 ttgtccatgg atcaaagggg catcgccagc ggtgggggtg atccactttg cgttccgcgc
      121 gatacaaaat ttttgagccg cgcgttcggt gggttcgaac tttgccatcg acagttgtca
      181 cacgtgctgc tgcgcgcctt ctcgagttac ccgctaatcg gcccgctgtt ccgagcgctg
      241 tccacgatgg gccctcggcg ttttgcgaag ctgatcggtg ccgccgtcgc aagaatgcca
      301 gatgcgtgat gttccgacca cgccgggttc ggagcgtact cgcgacgacc cggattcctc
      361 ccgcgcgccg cgaaatcccg gtcgtcgcga gtacgctgcg gacccggcgt gacatgttgc
      421 gcaagcatga ctttgcccgt cccctctggc ccgatcagtc cattagatta tgcccatgcg
      481 cgacagcgtt tcctgggtcc accatttcga accctacacc gccacgcacg cgtgtgtggt
      541 cgcggcgttt gtcatgctcg tcgcggccat ggtcgcgatg cgccggcgcg acgacatcgc
      601 caccgtcccg cccaatcgtc gcgcgatgga ttacgcgatc ggctatatcg gcctggcggc
      661 ggcggtgttc gtgcaggtgg tcacactctg gcccggccgg ttcgacgtgt ggacctcgct
      721 gccgctgcac gtgtgcgaca tctgcatgtt cgtcgcgccg ctatcgctgc tgctcggctg
      781 gcggccgctg cgcgcaatcg cgtacttctg gggattaggc ctgtcgtcga tgagcttcat
      841 ctttccggac ctgcgtttcg gcccgggcga ctttcagttc tgggtcttct ggaccggcca
      901 cgcgacgatc gtcggcaccg cgctatacga catcaccgcg cgcggctatc gcccgcggtt
      961 cggcgactgg tggatcgccg tcaagttcgg cgtcttctac gcggggacga tcttcttggt
     1021 cgatgcgctg tggcacttca attacggtta cgtgggccaa acgtacaaaa accagcgatc
     1081 gccggcggat ttcctcgggc cttggccgtg gcgcgtgccg ctgatggttg cgatggcgtt
     1141 cgcggcgatg ttcctgctgc tggcgccgtg gttgttgtgg cggcgacatg gcggcacggg
     1201 ctaccagtcc gcgattcgtg gtatgccacg aatgtcggcg ctattccacc gacgaagcga
     1261 tcgcgccagc gagggggttt gaaagccgtc gctgcgcgac tgctatgtcg ataactacat
     1321 cgactgcttg tgtacacgca ccaccggcag ctcgcccgtg ccacgcaaaa ttacgcgtga
     1381 tactgcgtgc tcccgttcca ctgctgcgtg ctgccggcgc cttgcggcat tccctgcggt
     1441 gacatcgcgt tggcctggct ctcggcaatc tgcgtcagcc gcttgtccgt ctcgccttct
     1501 tcgtccagcg tctgctggag gatctgcgcc gcatcgctga gacccaattg ctgcgccagc
     1561 gaacgaaccg tgccgtatgc ggccatctcg taatgctcca cgcgctgcgc cgcggcgatc
     1621 agcccggcat ccttcacgct gcagtcgccc tgggcgtcga tcatctcctg gccttccttg
     1681 attaagccgc gcatcgccgg acacgattcg gactcgggct ggcagttgat gctgcggaaa
     1741 actcgttcga gccgcgccac gtgttgctgg gtttgctgag cgtgcatctc gaaggccgac
     1801 ttcaactgcg acgatgtggc ggcgt
//
LOCUS       DATIMH010000684         5455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2473664, whole genome shotgun sequence.
ACCESSION   DATIMH010000684 DATIMH010000000
VERSION     DATIMH010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2473664"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VLI90_11970"
     CDS             <1..413
                     /locus_tag="VLI90_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14966.1"
                     /translation="VADFCLAMYGHMGLLDSIRVVRSSEPAFRAAACAIEDFYVDVPF
                     EGEIVRARLIDGQLKLHEGGDSYLTLPPAPFTKEQISPTRDSRLRWMQSVVQCTHYVS
                     GLGEQAYLHKADAPGITYVTRDNIERSDEAYTDV"
     gene            391..1128
                     /locus_tag="VLI90_11975"
     CDS             391..1128
                     /locus_tag="VLI90_11975"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSV14967.1"
                     /translation="MKPTPTSENAPASLLAVGAHPDDIEFGCGGVVAKETRIGRSAHF
                     VVCSQGEAASHGTPADRKAEAERAAAMLGASIEFLAFDGDGHLEMRVSHAIAIAQVIR
                     RAKPSVVLAPTTVENQHPDHSRLGRLTRDAARLARYGGVEELRSLPPHAIDQLLFYAV
                     TVEGEPRDVMPILVDVSEAEVMAAWTAAMAAHATQAAARNYVELQLTRAKLNGLRAGV
                     GHAIALFANDPLVVDSVSDLRRAARRF"
     gene            1288..2424
                     /gene="bshA"
                     /locus_tag="VLI90_11980"
     CDS             1288..2424
                     /gene="bshA"
                     /locus_tag="VLI90_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HSV14968.1"
                     /translation="MPQPTRLKIGITCYPSVGGSGILASALGEELARRGHEVHFICYE
                     RPFRLPADAPRIFFHAVEINDYGLFKYPDYTLPLSVRMDEVTREVGLDVLHVHYAVPH
                     ATAAVLARSMLPPDRQPRVVTTLHGTDTTLLGRDKGYGPAIRHALEQSDAVTTVSQYL
                     RKETQQVLGMTKPIDVIHNFFEPRQPTRSREEVRRELGLRQDQVLILHASNLRPGKRI
                     DVLLETAEQIQPSDAFKLVILAGDTFSPFAAEVDRLRLRDRVIVLERVNQIEDYLQAA
                     DIGLFTSDMESFCLSILEAMCFACPSVSTAVGGIPEVIEDGVTGVLAPAGDAAALARA
                     AEALIRDPAKRAALGAAARRVARERFSADAIVPRYEALYRRICG"
     gene            2791..>5455
                     /locus_tag="VLI90_11985"
     CDS             2791..>5455
                     /locus_tag="VLI90_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV14969.1"
                     /translation="MRTRRQAAAWLALAAVPVAIALSAGSAHAATDTWTGTTDSSWLT
                     PTNWTGGNAPPQAGDTIVFSGTTALSSDDNLGDGFSLAGIQFTTGAGAFVLSSHAGAN
                     DQALALTGPLLVNTGTSTAETINFAINGTSASSLSMGSDKSGGNSVVLGASDTFGLMS
                     VGTNSTTPDSLNIGATTLSVNGAVAIGSAPIAGTSTISTTILSVTGSGTWNVNSATNT
                     NFSVGVGANTNNNGNVEATLDMTGLAHFSFTTGTGNFGVGYKVTRPAAVAKLANTSNS
                     ITSALIGVGDSSQNGNGTDNNNNQNLANILDLGAGTNDFKTNTLIIGQTKTAGVVQYL
                     TPTGSVTIKGEAGGTSTTNITIANATGGTAPGLSSSLLLANNNATSATIQAGTVIVGN
                     SNGITGGTSTGLLTFDTGTFNATSLSLGTVSGGAFTALGGASGTLTLGTSSASTGVLT
                     VTGNFTLAGDTNTTANGGTATGKFIINGGTANLNSNVAITTLGTSVNSNTLATLTLAG
                     GTLNMNGFAICNPLAGGAFGTVTVNLPDTGQTATIANLGGNGIYATAGTGATSSGVVM
                     GGAGMLILAGNNTYAGPTTVSSGTLQIGSGNLGGSIPAASTFTNSSIVTFAGNVPITI
                     SNNITGSGVVNQNGSGATTLSGTNTYGGNTTINAGTLAVTGSISGNVSISPTNTGGTL
                     AGTGSVGNVTMSAAGAIHPGAGGADLNVGTLSLSSLNVTGGDMRFDIGGANSDAIVDS
                     GSATFTGGTISIALDSAPTLSSYTVLTASGGLSGSPILSTTSIGRTTFALVTVGNSLK
                     INISGGPATLTWNHVGGSGDGVTWNNTGNNQNWSKAGVADTFFDADNVTFNDTNGGHS
                     AVSITTQVQPGSVTFANSSASYVFSGAGGIAG"
BASE COUNT         1033 a         1600 c         1904 g          918 t
ORIGIN      
        1 acgtcgcgga tttttgcctg gcgatgtacg ggcatatggg actgcttgac tcgatccgcg
       61 tcgtccgttc gagcgagccc gcgtttcgcg ccgcagcctg cgcaatcgaa gatttttacg
      121 tcgatgtgcc gttcgaaggt gagatcgtcc gggcgcggct gatcgacggg caactgaagc
      181 tgcacgaagg cggcgactcg tacctgacgc tgccgccggc gccgttcacg aaggagcaga
      241 tcagcccgac gcgcgacagc cggctgcggt ggatgcagtc ggtcgttcag tgcacgcatt
      301 atgtctcggg cctgggcgag caggcgtacc tgcacaaggc ggatgcgccg ggaattacct
      361 acgtcacgcg ggataacatt gagcgatccg atgaagccta caccgacgtc tgaaaacgca
      421 cccgcttcgc tgctggcggt gggtgcgcat cccgacgaca tcgagttcgg ttgcggcggc
      481 gtcgtcgcga aggagacaag aatcggtcga tcggctcatt tcgtcgtttg ctcgcagggt
      541 gaagctgcga gtcacggaac gccggcggat cgcaaagccg aggcggagcg agcggcggcg
      601 atgcttggag cgtcgatcga attcctcgcg ttcgatggcg atggacatct tgagatgcgc
      661 gtgtcgcacg cgattgcgat cgcgcaggtg attcgccggg ccaagccgag tgttgtgctg
      721 gcgccaacga cggttgagaa ccagcatccc gatcattcgc ggctcgggcg gttgacgcgc
      781 gacgcggcgc ggttggctcg atatggcggc gtggaagaac tgcgatcgct gccgccgcac
      841 gcgatcgatc agttgctgtt ttacgcggtg acggtcgagg gagagccgcg ggacgtgatg
      901 ccgatcctgg tggatgtttc ggaagcggaa gtgatggcgg catggacggc ggcgatggcg
      961 gcgcacgcga cgcaggcggc ggctcggaat tacgttgagc tgcagctgac gcgagcgaaa
     1021 ttaaacggcc ttcgagccgg cgtcggtcat gcgatcgcgc tgtttgcgaa tgatccgctc
     1081 gttgtggatt ctgtttcgga tttgaggcgc gcagcgcggc gcttctagga cgctgccgct
     1141 agcggcttag catctgtctg gaagtcgaaa agatgccaag ccaaaagcgg ctgggatggc
     1201 gcgagggaac gttcatatac tactcggcca gatccttcga ctgcgcacga ataggtgctg
     1261 cgcccaggat gaactgtaag cgacaccatg cctcaaccca ctcgtttgaa gatcggcatt
     1321 acgtgctatc caagcgttgg tggcagcggc attcttgcgt cggcgctggg cgaggagctg
     1381 gcccgccgcg ggcacgaggt gcatttcatt tgttacgaac ggccgtttcg gttgccggcg
     1441 gatgcgccgc ggatcttctt tcacgcggtg gagatcaacg attacgggct gttcaaatat
     1501 ccggattaca ctctgccgtt gtcggtgcgg atggacgagg tgacgcggga ggtcgggctg
     1561 gacgtgctgc acgtgcatta cgcggtgccg cacgcgacgg cggcggtgtt ggcgcggtcg
     1621 atgctgccgc ccgatcggca gccgcgcgtg gtgacgacgc tgcacgggac ggacacgacg
     1681 ctgcttgggc gcgacaaggg gtatggcccg gcgattcgcc atgcgctgga gcagtcggac
     1741 gcggtgacca cggtgtcgca gtatctgcgg aaggaaacgc agcaagtgct cggcatgacc
     1801 aagccgatcg acgtcattca caacttcttc gagccgcggc aaccaacaag gtcgcgcgag
     1861 gaggtgcggc gggaactggg gcttcgtcag gatcaagtgc tgatcctgca cgcgtcgaat
     1921 ttgcggcccg gcaagcgcat cgacgtgctg ctggagacgg cggagcagat tcagccaagc
     1981 gacgcgttca agctggtcat cctcgccggc gacacgtttt cgccgttcgc agctgaagtc
     2041 gatcggctgc gtcttcggga tcgtgtgatc gtgctggagc gcgtgaacca gatcgaggac
     2101 tacctgcagg cggccgatat tgggctgttc acgtcggaca tggaaagttt ttgcctgagc
     2161 attctcgaag cgatgtgctt cgcgtgtccc agcgtgtcga cagcggttgg cgggataccg
     2221 gaagtgatcg aggatggagt cacgggcgtc cttgcgcccg cgggcgacgc cgcggcgctg
     2281 gcgcgggccg ccgaagcgtt gattcgtgat ccggccaagc gcgcagcgtt gggcgccgcc
     2341 gcgcgtcgcg ttgcgcgcga gcgcttttcc gccgatgcga tcgtcccgcg gtacgaggcg
     2401 ttgtatcgcc gcatttgcgg gtaaggcgac gctcggtcct ctctcttttc ccaccacagg
     2461 caaagcacga ataaagccgc gcatgttcat gacatgtaca tgcgggctcc ggcgccctcg
     2521 tgcgcgggtc ctgtcgcaaa gaggagaagg attggcgcaa aacaacgtgg ctcgggttgg
     2581 cgccgatgac gtacgctcct cctcgaatcg tggtcggcgc gaatcggaag cgcggaggtt
     2641 tggctgacca cgggggcgtc atttcatgcg gttgattgac acatagcttg gcggctcgat
     2701 ttcgcgcgcg acatgtcttt gcggagggtt cttatgcgga gtgattctcg atgcgcggcg
     2761 gcggcggtac ggtcacaaac attggccaac gtgcggaccc gacggcaagc ggcggcgtgg
     2821 ttggcgctcg cggcggtccc ggtggcgatc gcgttgtcgg ccggcagcgc gcacgcggcg
     2881 accgacacct ggaccggcac gaccgacagc agctggttga caccgactaa ttggaccggc
     2941 gggaacgcgc cgccgcaggc gggggatacg atcgtcttct cgggtacgac cgcgctgagc
     3001 tccgacgaca acctgggcga tggtttttcg ctcgccggga ttcagttcac aaccggcgcg
     3061 ggcgcgttcg tgctgagcag ccacgcgggg gcgaacgatc aggcgctggc gttgacgggg
     3121 ccgttgctcg tcaatacggg cacgagcacg gcggagacga tcaacttcgc gatcaacggg
     3181 acgagcgcca gcagcttgtc gatggggtcg gacaagagcg gcgggaacag cgtcgtgctg
     3241 ggcgcgagcg atacgttcgg gttgatgagc gtcggcacga attcgacgac gccggactcg
     3301 ttgaacatcg gcgccaccac gctgagcgtg aacggcgcgg tggcgatcgg cagcgcgccg
     3361 atcgcgggga cgagcacgat ctcgacgaca attctgagcg tcaccggcag cgggacgtgg
     3421 aacgtcaaca gcgcgacgaa caccaacttc agcgtgggcg tgggtgcgaa caccaacaac
     3481 aacggaaacg ttgaggcgac gctggacatg accggcttgg cgcacttctc gttcaccacc
     3541 ggcacgggga actttggcgt gggttacaag gtgacccgcc cggcggcggt ggcgaagctg
     3601 gcgaacacga gcaactcgat cacgtcggcc ttgatcggcg tgggtgacag cagtcaaaac
     3661 ggcaatggta ccgacaataa caacaaccag aacctggcga acatcttgga tctgggcgcg
     3721 ggcaccaatg acttcaagac caacacgctc attatcggcc agaccaagac cgcgggggtg
     3781 gtccagtatc tcacgccgac cggttcggtg acgatcaagg gcgaagcggg cgggacgagc
     3841 acgacgaaca tcacgatcgc caacgctacc ggcgggacgg cgccaggatt gagcagctcg
     3901 ctgctactgg cgaacaacaa cgcgaccagc gccacaatac aggcgggcac ggtcatcgtt
     3961 ggcaattcca acggcatcac gggtgggaca tcgaccggat tgctgacgtt cgataccggc
     4021 acgttcaacg caacgtcact ctcgctcggc accgtgagcg gcggggcgtt caccgcgctc
     4081 ggtggcgcca gcggaacgct gacgctcggc accagcagcg catcgaccgg cgtgctgacc
     4141 gtcacgggaa acttcaccct cgccggcgat accaacacca ccgccaacgg cggcacagca
     4201 acgggcaagt tcatcatcaa cggcggcacc gcgaacctga acagcaacgt ggccatcacc
     4261 acgttgggca cgagcgtaaa cagcaacacg ctggcgacgc tgacgctggc gggcggcacg
     4321 ctgaacatga acgggtttgc gatctgcaat ccgctggccg gcggcgcgtt cgggaccgtg
     4381 acggtcaacc taccagacac ggggcagacc gcgacgatcg cgaacctcgg cgggaacggc
     4441 atctatgcga cggccggcac gggcgcgaca agcagcggcg tggtgatggg cggggcgggc
     4501 atgttgatcc tggcgggcaa caacacgtac gcggggccga cgaccgtcag cagcggcacg
     4561 ctgcagatcg gctcgggcaa tctcggcggg tcgatcccgg cggccagcac gttcacgaac
     4621 agttccatcg tcacattcgc ggggaatgtg ccgatcacca tcagcaacaa catcaccggc
     4681 tcgggcgtgg tgaaccagaa cggcagcggc gcgacgacgt tgtccggcac gaacacgtac
     4741 ggcgggaata cgacgatcaa cgcgggcacg ttagcggtga cggggtcgat ctccggcaac
     4801 gtcagcatca gcccgaccaa caccggcggc acgctcgcgg ggacgggctc ggtcggcaac
     4861 gtgaccatga gcgcggccgg cgcgattcac cccggggcag gcggtgcgga tctgaacgtc
     4921 ggcacgttgt cgctcagcag cttgaacgtc accggcggtg acatgcggtt cgacatcggc
     4981 ggggcgaata gcgatgcgat tgtggacagc ggcagcgcaa cgtttaccgg cgggacgatt
     5041 tcgatcgcgc tggattcggc gccgacgctg tcgtcctaca cggtgctgac ggcgagcggt
     5101 ggcttgagcg gttcgcctat cctgagcacc acgtcgatcg ggcgaacgac tttcgcgctg
     5161 gtgacggtgg gcaattcgct gaagatcaac atcagcggtg gaccggccac gctgacgtgg
     5221 aatcatgtgg gcggcagcgg tgatggcgtg acctggaaca acacgggcaa caaccagaac
     5281 tggagcaagg ccggcgtggc cgacacgttc ttcgatgccg acaacgtgac gttcaacgac
     5341 accaacggcg ggcacagcgc cgtttcgatc acgacgcagg tgcaaccggg gtcggttacg
     5401 ttcgcgaaca gctcggccag ctatgtcttc agcggcgcag gcgggattgc cggga
//
LOCUS       DATIMH010000685        10211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1611579, whole genome shotgun sequence.
ACCESSION   DATIMH010000685 DATIMH010000000
VERSION     DATIMH010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611579"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(403..1890)
                     /locus_tag="VLI90_11990"
     CDS             complement(403..1890)
                     /locus_tag="VLI90_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSV14970.1"
                     /translation="MRILYIDIDSLRPDHLGCYGYHRKTSPNIDALAARGVRFDNHYA
                     SDVPCLPSRTALWSGQFGIHNGVINHGGVAAEPFEDGPSRQMDARIGQINWMRCLRRA
                     RPGLKAATISPFGERHGAYHWYAGFNEVYNTGRHGHERADEIAPIAIDWIGRNATLED
                     WFLHVNLWDPHTPYRTPESLGNPFANDPPPAWLTEDVRRTHWAQCGPHSAREMLGFTD
                     ARPDWAANCPLQPLSAASMDDVRRVFDGYDCGVFYADQHIGRIINALADHNLLDDTAI
                     IISADHGENLGELNIYGDHQTADECTCRIPLIVRWPGVTKSPRVDSALHYHFDFAATT
                     IELLGGKVPDVWDAQSFATAFRAGESSGREYLVLSQGAWSCQRAVRRGDHICVKSYHD
                     GYHLFPDVMLFNVRKDPHEQTNLTGEQPALANAMLALLDEWHEQMMRRAMHPIDRMTT
                     VIEEGGAFHTRGELPAYLERLRRTDRAACADELQRRHARDLQKSA"
     gene            complement(1992..3962)
                     /locus_tag="VLI90_11995"
     CDS             complement(1992..3962)
                     /locus_tag="VLI90_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HSV14971.1"
                     /translation="MSAIDRSSTSGLDHSTTVPARDAPSLRARIVKLLLGRPLASHEQ
                     EDVKIGVWAGIPAMGLDGLGSAAYGPEAALTMLLPLGALGLNYLGPITLVIIALLAML
                     YFSYRQTIAAYPVNGGSYTVAKENLGAWPGLLAAAALMMDYLLNVAVGISAGVAALVS
                     AAPALHRFTLALCLGILLVITLLNLRGTGEAGLVFALPTYLFIFSMMAVVVIGVARTF
                     SSGGHPAAIVAPPPIAPVTVTVGLWLLLRAFASGCTAMTGVEAVSNGVGAFKQPTVKN
                     AHRTLTAIVLVLALLLGGIAYLARAYHIGAMDQDKPGYQSLLSQLIGAVAGRGWAYYV
                     TIGSVLAVLCLSANTSFVGFPRLCRMIATDGYLPRAFTIVGRRLVYSVGVLVLAAAAG
                     LLLIVFRGITDRLIPLFAVGAFLAFTLSQAGMVAHWRRQDERSRSMTRICVNGAGAIA
                     TAVALAIIVAAKFVEGAWIVVVLIPCVLGLFKLIHRHYARVAERTIAGAPLDLNHNQP
                     PVVLVPTRGWDQLTGKALRFAMWLSTDVIAVHLSNLSGEEAEEADRRVRDEWRTQVEG
                     PARQHGVSPPKLEVVQAPYRSFLNPLLEEIDQLKSRYAGRQIAVVLPELIETRWWQLL
                     LHRRKPAKLRSALLKRGDHHVIVVDVPWYVDE"
     gene            complement(4223..5209)
                     /locus_tag="VLI90_12000"
     CDS             complement(4223..5209)
                     /locus_tag="VLI90_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14972.1"
                     /translation="MSRTFSTGSVRPAARAMRDKLGVCQWFHFQDYQAVERTTALLRE
                     LGVRMLRTGISWADFLRPGGREWYDWQMSKLANFDVLLSVWHTPPSIAEGNACNSPPR
                     RLRDYADFIDQVITLYGRRFSHLELWNEPNNRIKWDFERFDPQWRKFAEMIGAAAHWA
                     KHRGIRTVLGGMIPVDHHWLNLMRSHGLLQHIDVIGIHAFPGMWFPDHPNWDWHDHWK
                     GWDAKLAYIAEHTDGKPVWVTETGIATWDLALHREAKYELQVDALVSTAAAPAERVFW
                     YSLIDLDSERDAIEGFHVDENEYHMGLVKHDGYRKSAFATMQQLMSKKPRRA"
     gene            complement(5345..5893)
                     /locus_tag="VLI90_12005"
     CDS             complement(5345..5893)
                     /locus_tag="VLI90_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14973.1"
                     /translation="MTTRKTSSSGRSSGKSTGGRSGGGRMSGGSSRGKASSTQSTGRS
                     TGRASTRASGRTSSGGGGAAQMTIDHEQIRQWAEERGGKPTCVRGTGGKGDVGLLRID
                     FPGYSGEGKLEEIEWDEFFEKFEDQKLALLYQDSTKGGQPSNFNKLVSREDAGGKSGR
                     GRGKSGGGGGGRSRGSHGRGAK"
     gene            complement(5940..6845)
                     /locus_tag="VLI90_12010"
     CDS             complement(5940..6845)
                     /locus_tag="VLI90_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HSV14974.1"
                     /translation="MDDRRDMKDESTLTLTPSRRPAVSSTGRGDQRRRDLADDIERLG
                     PWFHNLHLPDGTQTCPDHWLGDFPLFKWRELAPHLPEDLSGWTALDIGCNAGFYTFEL
                     VKRGATVLGIDLDERYLRQARWAAEQFGVADRVSFEQLQVYDLARRAERFDLVLFMGV
                     LYHLRYPMLGLDIVSQRVNRLMVFQTLTMPGAEVFEQTWGRTFSADDRDRRLMLEPGW
                     PKMAFLEHGFARDPTNWWCPNHAGVEAMLRSAGMRITGHPGHEMYLCEPNPQRPSCVA
                     TWNAAELLAATGQPWLPAAGVERQG"
     gene            complement(6902..8035)
                     /locus_tag="VLI90_12015"
     CDS             complement(6902..8035)
                     /locus_tag="VLI90_12015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV14975.1"
                     /translation="MRNEVEQNPAAATPLRIVIIGLTITSSWGNGHATTYRGLVRELS
                     RRGHNVLFLEHDKPWYAGNRDLPNPPYGRTELYPSVAALKERFTHELSTADFVIVGSY
                     VPEGVSVGQWVTCTAQGVTAFYDIDTPVTLAKLQRGDFEYLSRDLIARYDLYLSFTGG
                     PTLRRLEQEYGSPRALPLYCSVDPELYYPESSPRIDCDLGYLGTYSDDRQPTLDALLL
                     QPARRWHEGRFIVAGPQYPVYIQWPTNVRRIEHLPPSQHRSFYNRQRFTLNVTRADMI
                     AAGYSPSVRLFEAAACGTPIVSDWWEGLETFFTPDEEILIARRAEDVLRYLKDVDEST
                     RLRIGRRARATVLSKHTAAHRALELERYAMQAIGSNGRAVHVD"
     gene            complement(8028..9146)
                     /locus_tag="VLI90_12020"
     CDS             complement(8028..9146)
                     /locus_tag="VLI90_12020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV14976.1"
                     /translation="MGDRMKIAFFGSSLVSAYWNGAATYYRGIIRALADRGHRVTFYE
                     SDAFDRQKHRDIPDPDWARVIVYSAADTGDVQRCLEVAAEADLIVKASGNGIFDELLE
                     DAVPNLKRPSNLVAFWDVDAPATLDRLHNNPLDPFRALIPQYDLILTYGGGDPVVRAY
                     EALGARRCVPIYNALDPSTHNPVAADARFEADLAFLGNRLPDREARVESFFLSAAAAL
                     PGKRFLLGGSGWADKPVPANVNYVGHVYTRDHNAFNCTPSAVLNVNRESMARYGFSPA
                     TRVFEAAGAGACVITDHFVGVETFFEPDTEIMVAKDGGEVAGHVDALTPQRAKAIGNA
                     ALRRALADHTYAHRAKLLEEVLGAAKSRSPTGAVLTHA"
     gene            complement(9131..>10211)
                     /locus_tag="VLI90_12025"
     CDS             complement(9131..>10211)
                     /locus_tag="VLI90_12025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV14977.1"
                     /translation="LFRGHDVRVYEPANAWSVQNLIAEHGEQPIADFHRAYPALDTVR
                     YEIDKLNLDDVLAEADVVLVHEWNDHALVHRIGQHSATRGSYQLLFHDTHHRAVTQPA
                     EMSKYDLTHYDGVLTFGQMLSDLYLKRRWTKRAWTWHEAADTRLFRPITGQPCDGDLI
                     WVGNWGDEERTAELQEFLLDPVKSLGLKARVHGVRYPDHATSSLAAAGIEYRGWLPNF
                     AVPDAFARFKLTVHVPRRPYVEALPGIPTIRVFEALACGMPLICSPWNDAEHLFEPGE
                     DYLIARDGREMRRHLKTLLDDPEYGRQLAAHGHRTILSRHTCAHRVDQLLNIIAELKV
                     ERPKRRRRQRGSRQVQRKEHRWVTA"
BASE COUNT         1801 a         3439 c         3147 g         1824 t
ORIGIN      
        1 gagatggggc gtaaacctgc cggagcggcg aaaaatccgg attttttctt taactgatgg
       61 caatagcgat ggttacagcc gtctgcagga ttgggatttg gggtgatgcc tggacgtgcg
      121 caagcgactg cgctgcggat cgcgcgctca ggaccagatc cttcgactgc gcacgagtac
      181 gtgctctgct ctaggatgac gcgctaccca gcccgttgcg cgcattcacc gtcgtcgtag
      241 atccatccgc gctgatgcgc tcttcgcttc actgatgtac cgcccgcggt gatccacgat
      301 ccgcgcggtg aacacctccg cctgccgcgt gcggccgtcg gattcgtccg cgatccatta
      361 cggcggcgcg ttcggcagcc cctcgtcgtt gtgctgcttg tcctatgcag acttctgcag
      421 atcgcgcgca tgtcggcgct gcagctcatc cgcgcacgcc gcgcgatcgg tccgccgcag
      481 gcgctcgaga tacgccggca gttcgccgcg cgtgtggaag gctccgcctt cttcgatcac
      541 cgtcgtcatg cgatcgatcg ggtgcatcgc ccgccgcatc atctgctcgt gccattcgtc
      601 cagcagcgcg agcatcgcat tcgccagcgc cggctgctcg ccagtcaggt tcgtttgctc
      661 atgcggatct ttccgcacgt tgaacagcat cacgtccgga aacaggtgat agccatcgtg
      721 atacgacttg acgcagatgt gatccccccg gcggaccgcg cgctggcacg accacgcgcc
      781 ctgcgacagc acgaggtatt cgcgaccgct cgattcgccc gcgcggaacg ccgtggcgaa
      841 gctttgcgcg tcccacacat ccggcacctt tccgccaagc aattcgatcg tcgtcgcggc
      901 gaagtcaaag tggtagtgca gcgctgaatc aacgcgcggg gacttggtca cgccaggcca
      961 gcgcacgatc agcgggatcc ggcaggtgca ctcatcggcg gtctggtgat cgccatagat
     1021 gttcaactcg cccagatttt cgccgtgatc ggcggagatg atgatcgcgg tgtcgtcgag
     1081 caaattgtga tcggccagcg cgttgatgat ccggccgatg tgctgatcgg cgtagaacac
     1141 gccgcagtcg taaccgtcga acacgcggcg cacgtcgtcc atcgacgccg ccgacagcgg
     1201 ttgcagcggg caattggcgg cccagtcggg ccgggcgtcg gtgaagccca gcatctcgcg
     1261 cgccgagtgc gggccgcact gcgcccagtg cgttcgccgc acgtcctcgg tcaaccacgc
     1321 cggcggcggg tcattcgcga acggattgcc cagcgactcc ggcgttcgat acggcgtgtg
     1381 cgggtcccat aggttcacgt gcaaaaacca atcttccaac gtcgcgttcc gcccgatcca
     1441 atcgatcgcg atcggcgcga tctcatccgc gcgctcatgt ccatgccggc cggtgttgta
     1501 aacctcgttg aaccccgcat accagtgata tgccccgtgg cgttcaccga acgggctgat
     1561 cgtcgccgcc ttcagtcccg gccgcgcgcg gcgaagacag cgcatccagt tgatctgccc
     1621 gatgcgcgca tccatttgcc gcgatggccc gtcttcaaac ggttcggccg ccacaccacc
     1681 gtggttgatc acgccgttgt gaatgccgaa ctgcccggac cacagagccg tccggctcgg
     1741 caaacacggc acgtcggacg cgtaatgatt gtcaaagcgc acgccccgtg ccgcgagggc
     1801 atcgatgttc gggctggtct tgcggtggta gccgtagcag ccaaggtgat cggggcgcag
     1861 ggaatcgata tcgatgtaga ggattcgcat ggggttcagc gacggataaa agtggtgtcg
     1921 ccaaacccat catcgtcgtt gaggtgtatt ggttcaaccg ggtcggcgtg gggagggttc
     1981 gcgggtgccc ttcattcatc cacataccac ggcacatcca cgacgatcac atggtggtcc
     2041 ccgcgcttca gcagcgctga gcgcagcttc gccggcttcc gccgatgcag cagcaattgc
     2101 caccagcgcg tctcgatcaa ctccggcagc accaccgcga tctgccggcc ggcgtagcgc
     2161 gacttcagtt ggtcgatctc ctccagcaac gggttcagga atgaccgata tggcgcctgc
     2221 accacctcaa gcttcggcgg gctcacgcca tgctgccggg ccggtccttc cacctgcgtc
     2281 cgccattcat cgcgcacgcg gcgatccgct tcttcggcct cttcgccact gagattgctc
     2341 aggtgcaccg cgatgacatc cgtcgacagc cacatcgcaa agcgcagcgc tttgccggtc
     2401 aattgatccc acccgcgcgt cggcaccagc acgaccggcg gctggttgtg gttcagatcc
     2461 agcggcgccc ccgcgatcgt ccgctcggcg acccgcgcgt aatgccggtg gatcaacttg
     2521 aacagcccga gcacgcacgg gatcagcacg acaacgatcc acgccccctc gacaaatttg
     2581 gcggcgacga tgatcgccag cgcgaccgcc gtcgcgatcg cgccggcgcc gttcacgcag
     2641 attcgggtca tcgatcgtga ccgttcatcc tgtcgccgcc agtgcgcgac catgcctgct
     2701 tgcgacaacg tgaaggcgag aaacgcgccg acggcgaaca gcggaatgag ccgatcggtg
     2761 atgccgcgga agacaatcag caacaacccc gcggcggccg ccaggacgag cacgcctacg
     2821 gaatacacga gccgccggcc gacgatcgtg aacgcgcgtg ggaggtaacc atcggttgcg
     2881 atcatccggc agaggcgagg aaatccaacg aagctggtgt tcgccgacaa acacagcacc
     2941 gccagcacgc tgccgatcgt gacgtagtac gcccacccgc gaccggcgac ggcgccgatc
     3001 agctgcgaca gcaggctttg atatcccggc ttgtcctggt ccatcgcgcc gatgtgatat
     3061 gcgcgggcca gatacgcgat gccgccgagc agcagggcca gcacgagcac gatggcggtg
     3121 agtgtgcgat gcgcgttctt caccgttggt tgcttgaacg cgccgacgcc gttgctgacg
     3181 gcttcgacgc cagtcatcgc ggtgcaaccg ctggcgaagg cgcggagcaa aagccataag
     3241 ccgacggtga ccgtcaccgg cgcgatcggc ggcggggcga cgatcgccgc cggatgtcca
     3301 ccgctgctga acgttctcgc cacgccgatc accaccacgg ccatcatgct gaagatgaac
     3361 aggtacgtcg gcagcgcgaa gacgagcccc gcttcaccgg tgccgcgcag gttcagcagc
     3421 gtgatgacca gcaggatgcc gaggcacaag gcaagcgtga atcgatgaag cgccggcgca
     3481 gccgacacca gcgccgccac cccggcggaa atgccgacgg cgacgttcag caggtaatcc
     3541 atcatcagcg ccgccgcagc gagtaagccc ggccacgcgc caagattctc cttcgccacc
     3601 gtgtacgatc cgccgttgac gggataggcg gcgatggtct ggcgatacga gaaatacaac
     3661 atcgccagca gcgcgatgat gaccagcgtg atcgggccaa ggtaattcaa ccccagcgcc
     3721 ccgagcggca gcagcatcgt cagcgccgcc tccggcccgt acgccgcgga gcccagccca
     3781 tcgagcccca tcgcggggat gccggcccaa acaccgatct tcacgtcttc ctgctcgtgc
     3841 gacgccagcg ggcgaccgag gagcagctta acgatcctcg ctctcagcga gggcgcatcg
     3901 cgcgccggca cggtcgttga atgatccagc cccgaggttg acgaacgatc gatcgcgctc
     3961 atcgaatagc agtcgcctta tccccctctc ccggtacgcc gggagagggc tggggtgagg
     4021 gtgatttcaa ttgacaattg aaatttgcaa ttgtcgattg tcagttccaa gacaaatcat
     4081 cctcacccta accctctccc ggtgtaccgg aagaggggac aagagggcgc gcgttctttc
     4141 taaggcgcgc tcaggcgacg cgaggacgca agcgacaacc gtgccaattt cgtgaggtgg
     4201 ggtttttcga acctcgcgag ctttacgctc gccgcggttt cttgctcatc aactgctgca
     4261 tcgtcgcgaa cgcacttttg cggtaaccgt cgtgtttcac gagccccatg tggtactcgt
     4321 tctcgtccac gtgaaaacct tcgatcgcgt cgcgctcgct gtcgaggtcg atcaggctgt
     4381 accagaaaac ccgctccgcc ggcgcggcgg cggtggacac cagggcgtcc acctgcagct
     4441 cgtacttcgc ctcgcgatgc agcgccaaat cccacgtggc gatgccggtc tcggtcaccc
     4501 acacgggctt gccgtcggtg tgctcggcga tgtacgcgag cttggcgtcc cagcccttcc
     4561 agtggtcgtg ccagtcccag ttgggatgat cgggaaacca catgccgggg aacgcgtgga
     4621 tgccgatgac atcgatgtgc tgcaacaagc catgcgatcg catcagattc aaccaatggt
     4681 gatccaccgg gatcatcccg cccagcaccg tgcgaatgcc gcggtgtttg gcccagtggg
     4741 cggcggcgcc gatcatctct gcgaacttcc gccactgcgg atcgaagcgt tcgaagtccc
     4801 acttgatgcg gttgttcggt tcgttccaca gctccaggtg cgaaaagcgc cggccataga
     4861 gcgtgatgac ttggtcgatg aagtccgcgt aatcgcgcaa ccgccgcggc gggctgttgc
     4921 acgcgttgcc ttcagcaatc gacggcggcg tgtgccagac gctgagcagc acgtcgaagt
     4981 tcgccagctt gctcatctgc cagtcgtacc attcgcgccc gcccgggcgg aggaaatccg
     5041 cccaggaaat gccggtgcgc aacatgcgca cgcccagctc gcgcagcagc gcggtggtgc
     5101 gttcaacggc ctggtaatcc tggaaatgaa accactggca gacgccaagc ttgtcgcgca
     5161 tcgcgcgagc ggcggggcgg accgatcctg tagagaacgt tcgactcatt ccggtcccct
     5221 cgcccggtac tccgggagag ggttagggtg agggtgattt cgagaaccag cggctctcga
     5281 aatactaaaa atcaccctca accctgccct ctcccacgcg tacgtgggag agggggtaac
     5341 aatttcactt ggcgccgcgt ccatgcgacc cgcggctgcg gccaccacca ccaccgccgc
     5401 tcttgccgcg gccgcggccg ctcttgccac cggcgtcttc gcggctcacc agcttgttga
     5461 aattactcgg ctggccgccc ttggtcgaat cctggtacag cagcgccagc ttctgatcct
     5521 cgaacttttc gaagaactcg tcccattcga tttcttcaag cttgccttca ccgctgtatc
     5581 cggggaagtc gatgcgcagc aggccgacgt cgcccttgcc accggtaccg cgaacgcacg
     5641 tgggtttccc gccgcgttct tcggcccatt gccggatctg ctcgtggtcg atcgtcatct
     5701 gcgccgcgcc accgccgccg ctggacgttc gcccggaagc gcgcgtcgat gcacggccag
     5761 tggatcgacc cgttgattga gtcgaagacg ccttaccgcg ggacgatcca ccggacattc
     5821 tcccaccgcc ggacctgccg ccggtggatt tgccggacga tctgccgctg gatgatgttt
     5881 ttcgcgtagt cataaggttg agcctcacaa agcaagcgtg gcaactgtgg gttgatcggt
     5941 catccctgac gctcgacacc agcggccggc agccacggtt gcccggtcgc cgcgagcaat
     6001 tccgccgcgt tccacgtcgc cacgcacgac ggccgctgcg gatttggttc acacaaatac
     6061 atctcatgac ccggatgtcc ggtgatgcgc atgcccgccg atcgcagcat cgcttccacg
     6121 ccggcgtgat tcgggcacca ccagttggtc gggtccctgg caaagccatg ctcgagaaac
     6181 gccatcttcg gccagcccgg ttccaacatc agccggcgat cgcgatcgtc cgcgctgaac
     6241 gtgcgccccc acgtctgctc gaacacctct gcccccggca tcgtcagcgt ttgaaaaacc
     6301 atcagccggt tcacgcgctg gctgacgatg tcgagcccga gcatcgggta acgcaggtga
     6361 tagagcacgc ccatgaacag cacgagatcg aaccgctccg cccgccgggc caggtcgtac
     6421 acctgcagct gctcgaagct cacgcgatcg gccacgccga actgctcggc cgcccatcgc
     6481 gcctgccgca ggtatcgttc atccagatcg attccgagca ccgtcgcgcc gcgcttgacc
     6541 agttcaaagg tgtagaatcc cgcgttgcag ccgatgtcca gcgcggtcca cccgctcagg
     6601 tcttccggca gatgcggcgc gagctcgcgc catttgaaca acggaaaatc gcccagccaa
     6661 tggtccgggc aagtctgcgt gccatcgggc aaatggaggt tgtggaacca cgggccgagg
     6721 cgctcgatat cgtcggctaa gtcgcggcgt ctctggtccc ctcgcccggt actgctgacc
     6781 gcaggtctcc gggagggggt tagggtgagg gtgctttcgt ctttcatgtc tcgcctgtca
     6841 tccataaatc accctcaccc cagccctctc ccacgcgtac gtgggcgagg gggcaagaac
     6901 gtcaatccac atgcaccgcc cggccattcg aaccaatcgc ctgcatcgcg tatcgctcca
     6961 gctccagcgc ccgatgcgct gccgtgtgct tcgacaatac cgtcgcgcgt gcccgccggc
     7021 cgatgcgcaa tctcgtgctt tcatccacat ctttcagata ccgcagcaca tcttccgccc
     7081 gtcgcgcgat taaaatctcc tcgtccggcg tgaagaatgt ctccaatcct tcccaccaat
     7141 cgctgacgat cggcgtgccg caggcggcgg cttcaaacag ccgcacgctc gggctgtatc
     7201 ccgcggcgat catgtccgcg cgcgtcacgt tcagcgtgaa gcgctggcgg ttgtagaacg
     7261 accgatgctg gctcggcggc aggtgctcga tccgccgcac gttcgtcggc cactggatgt
     7321 acaccggata ttgcggcccg gccacgatga atcggccctc gtgccaccgc cgcgccggtt
     7381 gcagcagcag cgcatccagc gtgggctggc gatcgtcgct gtacgtgccg aggtaaccga
     7441 ggtcgcagtc gatccgcggc gacgattcgg ggtagtacaa ctccggatcg acgctgcaat
     7501 acaacggcag cgcccgcggc gaaccgtatt cctgctcgag ccttcgcaac gtcggcccgc
     7561 cggtgaacga gaggtacagg tcgtatcgcg cgatcaggtc gcgcgagagg tattcgaaat
     7621 ccccgcgctg aagtttcgcc agcgtgaccg gcgtgtcgat gtcgtaaaac gccgtcacgc
     7681 cctgggcggt gcaggtgacc cactgcccga cgctcacgcc ttcgggcacg tacgagccga
     7741 cgatgacgaa gtcggcggtg ctgagttcgt gtgtgaatcg ctccttcagc gccgccacgc
     7801 tcgggtacaa ctcggtccgt ccatacggcg ggttcggcaa atcgcggttg ccggcgtacc
     7861 acggtttgtc gtgctcgagg aacagcacat tgtgcccgcg gcgggacagt tcgcgcacca
     7921 gcccgcgata ggtcgtcgcg tggccgttgc cccacgacga cgtgatcgtc aagccgatga
     7981 tcacgatccg cagcggcgtc gcggcggcgg ggttttgctc gacttcgtta cgcatgggtc
     8041 aacaccgcgc ccgtagggga ccgcgacttc gccgcgccga gcacctcttc caacagcttg
     8101 gcgcgatggg cgtacgtgtg atccgccagc gcgcggcgca gcgcggcgtt gccgatcgcc
     8161 ttggcgcgct gcggcgtcag cgcgtccaca tgccccgcca cctcaccgcc gtccttcgcc
     8221 accattattt ccgtgtccgg ctcgaagaag gtttcgacgc cgacaaaatg gtcggtgatc
     8281 acgcacgccc cggcgccggc ggcttcgaaa acgcgcgtcg cgggcgaaaa gccatatcgc
     8341 gccatgctct cgcggttcac gttcagcacg gcgctgggcg tgcagttgaa cgcgttgtga
     8401 tcgcgcgtgt aaacgtggcc gacgtagttc acgttcgccg gcaccggttt gtccgcccaa
     8461 ccgctgccgc cgagcaggaa gcgcttcccc ggcaacgccg cggcggcgga caggaagaac
     8521 gactcgacgc gcgcctcgcg atcgggcagg cggttgccga ggaacgccag atccgcttcg
     8581 aaccgcgcgt cggcggcgac gggattatgt gtcgacggat cgagcgcgtt gtagatcggc
     8641 acacaccgcc gggcgccaag cgcttcatac gcgcggacga cgggatcacc gccgccgtag
     8701 gtcaggatca ggtcgtactg cgggatcagc gcgcgaaacg gatcgagcgg attgttatga
     8761 agccgatcga gcgtggccgg ggcatcgacg tcccagaacg cgacgaggtt gctcggccgc
     8821 ttcaggttcg gcaccgcgtc ttccagcagc tcgtcaaata tcccgttgcc gctggctttc
     8881 acgatcaggt ccgcctcggc cgccacctcc aggcagcgct ggacgtcacc agtgtcggcg
     8941 gcggaataga cgatgacgcg ggcccagtcc gggtcgggaa tatcgcgatg cttctgccga
     9001 tcgaacgcgt cggattcgta gaacgtgacg cgatgcccgc ggtccgccag ggcgcggatg
     9061 atcccgcggt agtacgtcgc ggcgccgttc cagtacgccg acaccaggct cgatccgaag
     9121 aaggcgatct tcatgcggtc acccatcgat gctccttgcg ttggacttgg cgcgacccgc
     9181 gctgccgccg tcgccgcttg ggcctctcga ctttcaattc cgcgatgatg ttgagcagtt
     9241 gatcgacgcg gtgcgcgcac gtgtgccgcg acaggatcgt tcgatgtccg tgcgccgcca
     9301 gctgacgccc atattccgga tcgtccagca gcgtcttgag atgccgccgc atctcccggc
     9361 cgtcgcgcgc gatcagataa tcttcgcccg gctcgaacag gtgctccgcg tcgttccacg
     9421 gcgaacagat cagcggcatg ccgcacgcca gcgcttcgaa cacgcggatg gtggggatgc
     9481 ccggcagcgc ttcgacatac ggccggcgcg gcacgtgcac cgtcagcttg aaccgcgcga
     9541 acgcgtcggg caccgcgaaa ttcggcagcc agccacggta ttcaatgccc gccgccgcca
     9601 gcgacgacgt ggcgtgatcg ggatatcgca cgccgtgcac gcgcgctttc agccccagcg
     9661 acttcaccgg atcgagcaga aattcctgca gctccgccgt gcgctcttcg tcgccccagt
     9721 tgccgaccca gatcaaatcg ccgtcacacg gttgcccggt gatggggcgg aacaaccgcg
     9781 tgtccgccgc ctcgtgccag gtccacgcgc gcttggtcca ccgccgcttg aggtacagat
     9841 cgctgagcat ctgcccgaac gtcagcacgc cgtcgtaatg cgtgaggtcg tacttcgaca
     9901 tctccgccgg ctgcgtgacg gcgcggtgat gcgtgtcgtg aaacagcagc tgatatgagc
     9961 cgcgcgtcgc actgtgctgg ccgatgcggt gcaccagcgc gtgatcgttc cactcgtgga
    10021 cgagcaccac gtccgcctcc gccagcacat cgtccaggtt caacttgtcg atctcgtatc
    10081 gaacggtatc cagcgcggga tacgcccggt ggaaatcggc gatgggctgc tcgccgtgct
    10141 cggcgatcag gttctgcacg ctccacgcgt tggctggttc gtagacgcgc acgtcgtgtc
    10201 cgcggaacag c
//
LOCUS       DATIMH010000686         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5367020, whole genome shotgun sequence.
ACCESSION   DATIMH010000686 DATIMH010000000
VERSION     DATIMH010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5367020"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VLI90_12030"
     CDS             <1..403
                     /locus_tag="VLI90_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14978.1"
                     /translation="RRWRGLLCVTVAAAGVGGMAPRAGAAINTWTGGSATGNNWSDPA
                     NWGGTAPANNGTAAIVFAGSTRILPNIDTAFSVLSVTFNGGTSLMSQTGSNLTIGSGG
                     ITNNSSAVQDFGNAFVILGTSQTWNAASGPI"
     gene            587..826
                     /locus_tag="VLI90_12035"
     CDS             587..826
                     /locus_tag="VLI90_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14979.1"
                     /translation="MIGATGGAVDFSGATAYTTHFAGSGAGITASGNSTWTGGGISRI
                     ENDTNALLPITLNSASTAPAASATASLVTAAARCS"
     gene            817..2295
                     /locus_tag="VLI90_12040"
     CDS             817..2295
                     /locus_tag="VLI90_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV14980.1"
                     /translation="MFLSNPTIANTADFRLENAHLHVNKMADLGTGTTTLAGPLRSAG
                     NIDTAAELEFGGATASSTKALVLDSFGGGIWVSAGGTNLTLSGVISESGGTGRALSVY
                     GSSSTSVLSLTASNTYTGPTAINFGSVVSIPSFANGGVASPLGMSSSAPANLLLGGGF
                     FGGDGTLRYTGSSASTDRGATLSSAGNNCTIDVSTAATVLTMSGQFTGPGALTKAGIG
                     TLVMSGPTSNYANSTNVNAGTLTLASAGAITSSAITVSAAATMNINGSIPASTVLTDN
                     GTTNFAGNPSLVGAFTRPLSSLSIGNAAQAAVLQSASTLFPEILNVPTLSFTGTGKLD
                     LANNELTTTATPAAIRGNLAGGAIFTSRIGVGTTLGYADIGSGVTEVRYALRGDATLN
                     GTVDVGDLGALATNYGLAAGAIWSLGDFNYDGAVDVGDLGALATNYGTSLGASSSAGS
                     NGTSLAAPATTAASVPEPKSLGMVAITVAVGAMPRRRNRRCR"
     gene            2534..>2727
                     /locus_tag="VLI90_12045"
     CDS             2534..>2727
                     /locus_tag="VLI90_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14981.1"
                     /translation="MLIFARLGHYPLWDDEAITAMTARAVWRTGDTSARVDDHNLLAY
                     RNGLLLRDLKDRFTPPLQFYL"
BASE COUNT          495 a          945 c          841 g          446 t
ORIGIN      
        1 tcggcggtgg cgcgggttgt tgtgtgtcac agtggcggcg gccggtgtgg gcggcatggc
       61 accgcgagcc ggcgcggcta tcaatacctg gaccggcggc agcgcgaccg gcaacaactg
      121 gtcggacccg gccaactggg gcggcaccgc gccggcgaac aatggcacgg ccgccatcgt
      181 cttcgccggc agcacgcgaa tcctgcccaa tatcgacacc gccttcagcg tcctgtcggt
      241 cacctttaac ggcggcacgt cgctgatgtc tcagaccgga tccaacctga cgatcggatc
      301 cggcggaatc accaataaca gctcggccgt tcaggatttc ggcaatgcgt tcgtgatcct
      361 cgggacatcg caaacatgga atgccgcgtc tggccccatc tagtagacaa cagcatttcg
      421 ctcggttcca acgtgctgac cacggccggg agcggcggtt cgcaaacagc ggaagcgttc
      481 gggggaatca tcagcggaag cggcatcgga aatcggcacg ctgacgctga acagcggcac
      541 gttcaaaacc agcggcggcg ccggtgactt caatctgaac cagcttgtga tcggcgcaac
      601 cggcggcgcg gtggattttt ccggtgcaac ggcttacacc acgcacttcg ccggcagcgg
      661 cgcgggcatc actgccagcg gcaactccac ctggaccggc ggtggcatat cgcgcatcga
      721 gaacgacacc aacgccctgc tgccgatcac cctgaacagc gcctcaacgg ctccggcggc
      781 ttcggctacc gcatcactgg tgacggcagc ggcacgttgt tcctgagcaa tccaaccatc
      841 gccaacaccg ccgacttccg cctcgaaaac gctcatctcc acgtgaataa gatggccgat
      901 ctgggcacgg gaaccaccac gctggccggg ccgcttcgct cggctggaaa tatcgatacc
      961 gccgccgagt tggagtttgg cggcgcgacg gccagttcga ccaaggcgct ggtgctcgac
     1021 agtttcggtg gcggcatctg ggtcagcgcc ggcggcacga acctgaccct cagcggtgtg
     1081 atcagcgaga gcggcggcac cggccgcgcg ctgtctgtct atggcagttc cagcacatcg
     1141 gtcctgtcgt tgacggcatc gaacacgtac accgggccga cggccatcaa ctttggcagt
     1201 gtcgtgagca tccccagctt cgccaacggc ggcgtcgcca gcccgctggg catgtcgtcc
     1261 agtgcaccgg ccaacctcct gcttggcggc gggtttttcg gcggcgatgg cacgcttcgc
     1321 tacaccggat catccgccag caccgatcgt ggcgcgaccc tcagcagcgc tggcaacaac
     1381 tgcacgatcg acgtgtccac ggctgcaacc gtgctgacga tgtccggcca attcaccggc
     1441 cccggcgcgc tgaccaaggc cggtatcgga actttggtga tgagcggccc caccagtaac
     1501 tacgccaaca gcacgaacgt gaacgccggc acgctgactc tcgcctccgc aggcgccatc
     1561 accagcagcg ccattaccgt ctcggcggcg gcgaccatga acatcaacgg ctccattccc
     1621 gcgagcacag tcctcaccga caacggcacg accaacttcg ccggcaaccc ctcgttggtc
     1681 ggggcgttca cccgcccgct gtcgtccctc agtatcggca acgcggcaca ggcggcggtt
     1741 cttcagtcag cgtctacgct attccccgaa atcctcaacg ttccaacgct gagcttcacc
     1801 ggcaccggca agcttgacct ggccaacaat gagttgacca cgacggcgac gcccgcggcg
     1861 attcgcggca acctggccgg cggtgcgatc ttcacgtcgc gcatcggcgt cggcaccacc
     1921 ctcggttacg ccgacatcgg tagcggtgtg acggaagtga ggtatgcgct gcgcggcgat
     1981 gcgaccctca acggcacggt tgacgtcggc gacctgggcg cgctggctac aaactacggc
     2041 ttggccgcag gcgccatctg gtcgctaggc gacttcaatt acgacggcgc cgtggatgtg
     2101 ggcgacctgg gcgcgctggc aacgaactat ggcacgagct tgggcgcgag cagcagcgcg
     2161 ggcagcaatg gaacttccct tgcagcaccc gccaccacag cggcttcggt gcccgagccg
     2221 aaatcgcttg gcatggtcgc gatcaccgtc gcggtgggcg caatgccacg ccggcggaat
     2281 cgacgctgcc gttgatcgtc attccccctg ggtgatcgaa caactgctac gcacgcgcgc
     2341 tggctgctcg acaatgagga gcgttcgcga gacagttgca cgggtgaacg tgaaggtgcc
     2401 agcaaacgtc ggtcgcgaac ggggcgaggc ggatcgggat cgcacgccaa caccgtccgc
     2461 tcccgccgca ccatcgcgac ggtccgccgg accaaccgct cttgcgacgg tcgccattct
     2521 gctcgcatcc gcggtgctca tcttcgcccg cctcgggcac tatcccctct gggacgatga
     2581 agccatcacc gcgatgaccg cccgcgcggt ctggcggacc ggcgacacct ccgctcgcgt
     2641 ggacgatcac aacctgctcg cctatcgcaa cggcctgctg ctgcgcgatc tcaaggaccg
     2701 cttcacgccg ccgctgcagt tctatct
//
LOCUS       DATIMH010000687         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1611934, whole genome shotgun sequence.
ACCESSION   DATIMH010000687 DATIMH010000000
VERSION     DATIMH010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611934"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VLI90_12050"
     CDS             <1..1116
                     /locus_tag="VLI90_12050"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HSV14982.1"
                     /translation="VVMSRAGHLLPAVALALLAAAAPVVAHHEIAAKFDESKATTLTG
                     IVTDVDWKNPHVHVLMNVKTGADVKNWAVELESPLALEQSGWASDTLKPGDSITVQGI
                     AARNGSRQVWSKSITLKSGKQVLAMKTIAPPPATHKPTPRTADGKPRLGFEPGKMGYW
                     AFPSSTVLVEKNANIPMDGYGLLKNIADASKVAPMQPWALALYRERQKRFLQDDPTFI
                     NCKPPGGPRQFQQPEGVQFVEDSARQRMFLLLGGGNSNYRILWMDGRGHTGQIAGDDD
                     NPLYYGRAVAQWEGDTLVVDTRGFNEDFWFTNGGLPHTDQLKLVERFTRPDFDTLRYD
                     VTVDDPGAYTRPWSTGWTLRWVADQELPKHFCQDNRP"
     gene            1132..1554
                     /locus_tag="VLI90_12055"
     CDS             1132..1554
                     /locus_tag="VLI90_12055"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HSV14983.1"
                     /translation="MKAYLLGGLMVCGLLLAAGPSSAHHSFTAEFDGNKPVTLTGTVT
                     KVEWTNPHVWFYINVKDDKTGQVTNWGAEMGPPHGLQRRGWRRESLKVGDVVTVKGSM
                     AKNGSNRMNASAVTLTGSGLRPGETLDAASSQGGNADQ"
     gene            1685..>2033
                     /locus_tag="VLI90_12060"
     CDS             1685..>2033
                     /locus_tag="VLI90_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14984.1"
                     /translation="MSNRTSLLLGAALIAGAVSTAPILAQSQGGNQQQPAAQSQGGAD
                     QGRPGVQVGVPSGGRDQGGAAQGGARAGGRGRQAGPPPAPAPRNAQGRVLLGGANAKD
                     KGVWLPAGVSIVQP"
BASE COUNT          410 a          647 c          654 g          322 t
ORIGIN      
        1 gttgtgatgt cccgcgcagg acacctcctg ccagccgttg ccctcgcact gctcgccgct
       61 gcggcgcccg tcgtcgcaca ccacgaaatc gcggccaagt tcgacgagtc caaagcgacc
      121 acgctgacgg gcatcgtcac cgacgtcgat tggaagaacc cgcacgttca cgtgctgatg
      181 aacgtgaaga ccggggcgga cgtgaagaac tgggccgtcg agctcgaaag tccgctggcg
      241 ctcgaacaga gcggctgggc gagcgacacg ctcaagcccg gcgacagcat caccgttcag
      301 ggcatcgccg cccgcaacgg aagccgtcag gtctggtcga agtcgatcac gctgaagagc
      361 ggtaaacagg tgctggcgat gaagacgatc gcgccaccgc ctgctaccca caaacccacg
      421 ccgcgaaccg ccgacggcaa gccgcgcctg ggtttcgagc cgggcaagat gggctactgg
      481 gcgttcccga gttcgaccgt gctcgtcgag aagaacgcaa acatcccgat ggatggttac
      541 ggccttctga agaacattgc cgacgcgagc aaggtcgctc cgatgcagcc gtgggcgctg
      601 gctctctacc gggagcgaca gaagcgtttc ctccaggacg acccgacctt catcaactgc
      661 aagccgccgg gcggaccccg tcagttccag cagcctgagg gtgtgcagtt cgtggaagac
      721 tccgcccgtc agcgcatgtt cctgctcctc ggtggcggca acagcaacta ccgcatcctg
      781 tggatggatg gccgcggcca taccggacaa atcgccggag acgatgacaa cccgctgtac
      841 tacgggcggg ctgtggcaca atgggaaggc gacactcttg tcgttgatac cagagggttt
      901 aatgaagatt tctggtttac caacggcggc ctcccacata ccgatcaact caaactcgtt
      961 gagcgcttca cgcgcccaga cttcgacacg ttgcggtatg acgtaacggt tgacgatcca
     1021 ggggcgtata ccagaccgtg gtcgaccgga tggacgctgc gctgggttgc cgatcaggaa
     1081 ctgcccaagc acttttgcca ggacaataga ccgtaaagac ggaggaacga gatgaaggcg
     1141 tatttactcg gtggcttgat ggtttgcgga ctcctgctgg cagcaggtcc ttcctcggcc
     1201 caccattcgt ttaccgcgga gttcgacggc aacaagccgg tcacgctgac cggaacggtg
     1261 acgaaggttg agtggaccaa ccctcatgtg tggttctaca tcaacgtcaa ggatgacaag
     1321 accggccagg tcaccaactg gggcgccgaa atgggcccgc cgcacggcct gcagcgccgc
     1381 ggctggcgcc gtgagtcact caaggtgggc gatgtcgtca cggtcaaggg ttcgatggcc
     1441 aagaacggca gcaatcgcat gaatgccagc gcggtgacac tcaccggcag cggccttcga
     1501 cccggcgaaa cgctcgacgc cgcgtcgagc cagggcggca acgccgacca atagttcgcg
     1561 attggcgaat cggtgattcg cgactggcag cgggaacgcg ccagtcgcgg gtcacgacat
     1621 cgcgtctcgc ggatgaagac ccgatcacgt tcacagttca ttcataccaa atcacatcca
     1681 atcaatgagt aatcgaacat cgttgctgct tggcgcggcg ttgatcgcgg gagcagtgtc
     1741 gaccgcaccg atcctcgcgc agagccaggg cggcaatcag cagcagcccg ctgcgcagtc
     1801 gcagggtggc gccgatcagg gccgcccggg cgttcaggtg ggcgtgccat ctggtggccg
     1861 cgatcagggc ggcgccgcac agggcggcgc gcgcgccggc ggccgcggac gccaggcggg
     1921 accacctccg gcccccgcac cccgcaacgc gcagggccgc gtgctcctcg gcggtgcgaa
     1981 tgcgaaggac aagggcgtct ggctcccggc cggcgtcagc atcgttcagc cgg
//
LOCUS       DATIMH010000688         5564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4789864, whole genome shotgun sequence.
ACCESSION   DATIMH010000688 DATIMH010000000
VERSION     DATIMH010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4789864"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VLI90_12065"
     CDS             <1..457
                     /locus_tag="VLI90_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14985.1"
                     /translation="NTSHLPNHKLAVKNDLSASPASANTATEIGVIQTADRQVILSEP
                     QRQVLEWLYNEEGATVKDAAEFAGVGRATIFRWIQADPDFNALYTAWRQQQEQAKEVQ
                     LSGLEAAALNVLNDAVRRRGDVRAAQFILKQMTARWIRKEKSSSPSDSL"
     gene            complement(516..944)
                     /locus_tag="VLI90_12070"
     CDS             complement(516..944)
                     /locus_tag="VLI90_12070"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSV14986.1"
                     /translation="MLKLSPHQFGTIFDTLRATGSSRGSEKRQATRMDVQTKLSLAKM
                     VDGNVVRCYSALSRDISCTGLGFFQFAPLANGERFLCCFPFGKEELVLVCSSMFCRPL
                     AEGLFGIGAQFECAADQKLIDQLKQQRATDVDRIRASILT"
     gene            1487..1807
                     /locus_tag="VLI90_12075"
     CDS             1487..1807
                     /locus_tag="VLI90_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14987.1"
                     /translation="MRHSSAAAFVLLTLGMLLSGCGSQEGETVFTAGPNNAEVVGKAP
                     HTGTYLLFTAASPNPTTTVNLNEGDPMGFHKTADGKIQAFWKDQTYDFDKATAQVYWK
                     YKKS"
     gene            1835..2185
                     /locus_tag="VLI90_12080"
     CDS             1835..2185
                     /locus_tag="VLI90_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14988.1"
                     /translation="MPGKHSRTKFARLGTVLSLALVAGVPLVATSCGAQGGRTIMTQG
                     ANAEPIMGTAPEAGTYKLYTAFSPNPTTTVNLKQGDQLGFRRDDNGQLVAVAGDQTQP
                     LAKGTSQAYWKLEK"
     gene            complement(2279..2575)
                     /locus_tag="VLI90_12085"
     CDS             complement(2279..2575)
                     /locus_tag="VLI90_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HSV14989.1"
                     /translation="MVQFALYAHMKAKPGKAAELEAFLKAALPLAEKEPGTVTWYAFQ
                     EDEGSYGIFDTFETEAARQAHLDGPIAKALMSKAAELLSQPPAIHKIRVLAAKR"
     gene            complement(2692..3927)
                     /locus_tag="VLI90_12090"
     CDS             complement(2692..3927)
                     /locus_tag="VLI90_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HSV14990.1"
                     /translation="MPMRCFILLALLCALLPCGCSSSPDLKPADFSGHTPIVRVLILQ
                     GITSVELTATRPPIVTTFSVPSPHRLDVAPATPVPLTLSPVGWRLGNVIVGTGEMQIQ
                     PSGEGTVAFDRQSYRGRFRLVPTGPARFDVVNDVDVEGYLKGVLPRELKRFWNDQTYK
                     AQAVVARTYAIYELKAASSASHYDVFDDQRSQVYGGIGAESAKARDAVDATAGWVVAS
                     GPPGQERIFKAYFSSCCGGIGQSAADAFGDPPSEPLSEQAVGTLCSESPHFSWGPVVV
                     SKADLTKRFRHFGAIRKRAEKDMADVVRIDVAVNNKVGRPVRFVATDAKGAHYFWTGE
                     ELRWAVNTDATDTTRLMSSLLQIDNSQPGIIRFTNGHGMGHGVGLCQWCAQRRAELGM
                     RYDAIVLSAFPNSKLIRAY"
     gene            complement(4053..4985)
                     /locus_tag="VLI90_12095"
     CDS             complement(4053..4985)
                     /locus_tag="VLI90_12095"
                     /inference="COORDINATES: protein motif:HMM:NF015102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HSV14991.1"
                     /translation="MSVASINERAAEEYFRRGLDAEKEGNHEKAVEFYERALNENPDH
                     EQGAFRLAVLYDRRAEDAKAIELYERICTSPPVHLNALMNLAVLYEDNNHYDEAHRCL
                     DAILRTDPNHQRARLYMKDVESARSMYYDDDDHRAGGRNAVLEIPISDFELSVRSRNC
                     LKKMNIKTLGDLLRTSEQELLSYKNFGETSLNEIKALLAQKGLRLGQNLEGGAATPTS
                     SSAPAAPGAVARRPAVIAGDVPQEILAKTVGDMELSVRSRKALQRLNINTVGELAART
                     EDELLGCKNFGQTSLNEIKQQLTAMGLGLRKLED"
     gene            complement(5262..>5564)
                     /locus_tag="VLI90_12100"
     CDS             complement(5262..>5564)
                     /locus_tag="VLI90_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14992.1"
                     /translation="RPQWRTSDELTLNVFEIQHGSDVTARVAASDGASTTFRGRRDGQ
                     TLRFDGDGSARNVRVLLRSNRAARGLVNGKLVGEMPEGLAIEWLDPGKPLSLTLLE"
BASE COUNT         1025 a         1817 c         1711 g         1011 t
ORIGIN      
        1 gaacacatca cacctgccca atcataagct cgccgttaaa aacgacttat ccgcatcgcc
       61 cgcatctgcc aacactgcga cggagattgg tgtcatccag accgccgatc gccaagtcat
      121 tctcagcgag ccgcagcggc aggtcttgga atggctgtat aacgaggaag gcgcgacggt
      181 taaggatgcc gccgaattcg ccggtgtcgg gcgagccacc atcttccgct ggatccaagc
      241 cgaccccgac ttcaacgcgc tctacacggc ttggcggcag cagcaggagc aggcgaagga
      301 ggtccagctg tccggcctgg aggccgccgc cctcaacgtc ctcaacgacg ccgtccgccg
      361 ccgcggcgat gtgcgcgccg cgcagttcat cctgaagcag atgacggcgc gctggattcg
      421 gaaggaaaaa tcgtcgtcgc cgtccgacag cctgtaaacg aagaagtgcc agcggggctg
      481 agcgtgcgcc tgggcccttt ggcacgggcg aacactcagg tgaggatcga cgcgcggatg
      541 cgatcgacgt ccgttgcgcg ctgctgcttc agctggtcga tcagcttctg atcggcggcg
      601 cactcgaact gcgcgccgat gccaaacagt ccctccgcca gcgggcggca gaacatcgac
      661 gagcagacca ggaccagctc ttccttgccg aacgggaagc agcacaagaa tcgttccccg
      721 ttggccaggg gcgcgaattg gaagaagccg aggcccgtac aggaaatgtc gcgcgagagc
      781 gcgctgtagc agcggacgac gttgccgtcc accattttgg ccaggctcag tttcgtctgc
      841 acgtccatgc gcgtcgcctg gcgcttctcg ctgccgcgcg acgaaccggt cgctcgaagg
      901 gtgtcgaaga tcgttccaaa ctggtgaggc gaaagcttca acatcggtcc accccggata
      961 acggaagaca tcaaagaccc tatcggcaca aatcgagata cgtgtaactg gggcgacaaa
     1021 cctgatcgcg ccggccgtcc gcaaagatcg gctgagtcga gcgaatcgat attctcggac
     1081 gtctaactcg gcgttaacgt cagcgtgctt ggcgcgccat tgattccggc gacaatgctg
     1141 aacatcgcgg cgtgtgccca cgcggcccgt atggtgtctg ggggaggagg taatggattg
     1201 ctttcccttc acgcctgggg cgaggtcatc gtgatctcga tactgctgac cgccacgctg
     1261 cacgtcctgc tcgaacgcca tcggcgtaac aagtagcgca ccgagcgcgc ggtctgttcg
     1321 ccccccacgc gcgccccagg cgccccacgc ggcgccgatt tctgccgttc gcgcttcgcc
     1381 gtcctctgct taatcttcga actgtagttt gttgcgatcg cgcgaccgcg gcgccaccga
     1441 cacgcccggc cgcgcgcgct ggtttgcgtt cgcgaaaggc aatcaaatgc gacattcatc
     1501 tgcggcggcg tttgtcctgc tcaccttggg gatgctcctg agcggttgcg gctcgcagga
     1561 aggcgagacc gtcttcaccg ccgggcccaa caacgcggag gtcgtcggca aggcgcccca
     1621 caccggcacc tatctgctgt tcaccgcagc cagccccaat ccgacgacga ccgtcaatct
     1681 gaacgaaggc gacccgatgg gcttccacaa gacggccgat ggaaaaatcc aggcgttctg
     1741 gaaggatcag acctacgact tcgacaaggc gaccgcgcag gtctactgga agtacaagaa
     1801 gagttgatca ccccaagaga aggagttcac gcacatgcca ggcaaacatt cacgcacgaa
     1861 gttcgcgcgc ttgggaacgg tgttgtcgct cgcgctggtc gcgggcgtgc cgctcgtcgc
     1921 caccagctgc ggcgcacagg gcggccggac gatcatgaca caaggcgcca acgccgaacc
     1981 aatcatgggc accgcccccg aagccggcac gtacaagctg tacaccgcgt tcagcccgaa
     2041 cccgacgacg accgtgaacc tcaagcaagg cgaccagctc ggcttccgcc gagacgacaa
     2101 cggccaactc gtcgcggtcg ccggcgatca aacccaaccg ctggccaagg gcacgtccca
     2161 ggcgtattgg aagctggaga agtgactgtt tgagaatcca gaatgaagaa tgcagaatga
     2221 agacgggacg agccgcttca ttctgcattc tcattcatgc ttctgcattc cgcccacctc
     2281 accgcttcgc ggcgagcacg cggatcttgt ggatcgccgg tggctgcgac agcagctcgg
     2341 ccgcctttga catcagcgct ttggcgattg gtccatcgag gtgcgcctgc cgggcggctt
     2401 cggtttcgaa cgtgtcgaag atgccgtacg agccttcgtc ttcctgaaac gcataccacg
     2461 tgacggtgcc cggctccttc tccgccagcg gcagcgcggc cttcaagaac gcttcgagtt
     2521 ccgcggcttt gccgggcttg gctttcatgt gcgcgtagag ggcgaattgg accatgggga
     2581 tctccttcgt ttgtcgaggt gcggcccacg tcgcgccggc gacggagcga gatgcaattg
     2641 taggcgctct tgctccctcg cccacgtatc cgtgggcgag gggaccggag attaatacgc
     2701 gcggatgagt ttcgaattgg gaaacgccga caggacgatc gcgtcgtaac gcatgcccag
     2761 ctccgcccgg cgttgggcgc accactggca cagccccacg ccgtggccca tgccgtggcc
     2821 gttggtgaag cggatgatgc ccggctggct gttgtcgatc tgcagcaggc tgctcatcaa
     2881 gcgcgtcgtg tcggtcgcgt cggtgttgac ggcccagcgc agctcttcgc cggtccagaa
     2941 atagtgagcg cccttggcat ccgtcgcgac gaagcgcacc ggccggccga ccttgttgtt
     3001 gacggcgacg tcgatgcgca cgacgtcggc catgtccttc tcggcgcgct ttcgaatcgc
     3061 accgaagtgg cggaagcgtt tggtgagatc ggccttgctg acgacgaccg gtccccagct
     3121 gaagtgcggc gactcgctgc acagcgtgcc gaccgcctgc tcgctgagcg gttcgcttgg
     3181 cggatcgccg aacgcgtcgg cggcgctttg gccgatcccg ccgcagcagc tgctgaagta
     3241 cgccttgaag atgcgctcct gccccggcgg acccgacgcg acgacccagc ccgccgtcgc
     3301 atccaccgcg tcgcgcgcct tggcggactc cgcgccgatc ccgccgtaca cctggctgcg
     3361 ctgatcgtcg aacacgtcgt aatgcgatgc gctgctggcc gccttcagct cataaatcgc
     3421 gtacgtgcgc gccaccaccg cctgcgcttt gtacgtctga tcgttccaga agcgcttgag
     3481 ttcccgcggg agcacgccct tgagatagcc ctcgacatcc acgtcattga cgacgtcgaa
     3541 ccgcgcgggg ccggtgggca cgaggcggaa tcggccgcga tacgattggc gatcgaacgc
     3601 gacggtgcct tcgcccgatg gttggatctg catttcgccc gtgccgacga tgacgttgcc
     3661 gagccgccag ccgacgggac tcaacgtcag cggcaccggc gtcgccggcg caacgtccag
     3721 ccgatgcggc gacggcacgc tgaacgtcgt gacgatcggc ggtcgagtcg cggtgagttc
     3781 aacgctggta atgccctgca ggatgagaac gcggacgatc ggcgtgtgac cggaaaaatc
     3841 ggcgggcttc agatcaggac tgctgctgca cccgcagggg agcagcgcgc acaacagagc
     3901 gagaaggatg aaacatcgca tgggcatccc tgccttcgcg tgcgcgccat cggaaccgga
     3961 acaaaagcgc aataaagtcc acggctccgc cgtgggacgc atttttcagc gcccggtccc
     4021 acggaaaagc cgtgggcttc tattcggacg actcaatcct caagcttccg cagccccagc
     4081 cccatcgccg tcagctgctg cttgatctcg ttcaagctgg tctgaccgaa gttcttgcag
     4141 cccagcaatt cgtcttccgt gcgcgcggcc aattcgccaa cggtgttgat gttcagccgc
     4201 tgcagcgcct ttcggctgcg caccgacagt tccatatcgc caaccgtctt ggccaaaatc
     4261 tcctgcggca catcaccggc gatcaccgcc ggccgacgcg ccacagcgcc cggcgccgca
     4321 ggcgcggacg acgacgtcgg ggtggccgcg ccgccttcca ggttctgccc cagccgcagg
     4381 cccttctggg ccagcagcgc cttgatctcg ttcaggctcg tctcgccgaa attcttgtag
     4441 ctgagcagtt cctgctcgct ggtgcgcaac aggtcgccca gcgtcttgat gttcatcttc
     4501 ttcaggcaat tccggctgcg gaccgacagc tcgaaatcgc tgatcgggat ctccagcacc
     4561 gcgttgcgac cgccggcgcg atgatcgtcg tcatcgtagt acatgctgcg ggccgactcg
     4621 acatccttca tgtagagccg ggcgcgctgg tggtttggat cggtccgcag gatcgcatcg
     4681 agacagcgat gcgcttcgtc gtaatggttg ttgtcttcgt acaggacggc caggttcatc
     4741 agcgcgttca aatgcaccgg cgggctggtg cagatgcgct cgtacagctc gatggccttc
     4801 gcgtcttccg cccggcggtc gtacaacacc gccagccgga acgccccctg ttcgtggtcg
     4861 ggattctcgt tcagcgcccg ctcgtaaaac tcgaccgcct tctcgtggtt gccctctttt
     4921 tccgcatcca acccgcggcg gaaatactct tcggcggccc gctcattgat cgacgctacg
     4981 ctcatttcac tcatatcggt gttcgctcgt gtgtgatgac gttcgggacg cggcgatgat
     5041 gctctcaaca gcgcatgggc aatcgccgcg ccagttccgg tgcaaacagt aattgatacg
     5101 acgcgaggac gccggtgtca acgtcgaggc agaggataga ccatgatctc ggtacgtccg
     5161 gagaggttac ggtgagggtg attccggaaa tgaccaatga gcaagcatca aatcccagtg
     5221 aatgcccgat cagccgatcg caatattgac ggtcgcccga ttcattcaag caacgtcagc
     5281 gacagcggct tgcccgggtc cagccattcg atggccagcc cttcgggcat ttcgcctacg
     5341 agcttgccgt tgaccaaccc gcgggcggcg cgattcgagc gcagcagcac gcgaacgttg
     5401 cgcgctgagc cgtcgccgtc gaatcgcagg gtctgaccgt cgcgccggcc gcggaaggtg
     5461 gtggatgcgc cgtcactggc ggccacgcgc gctgtcacat ccgagccgtg ctggatttcg
     5521 aaaacgttca acgtcagctc atcgctcgtc cgccactgcg gtcg
//
LOCUS       DATIMH010000689         1630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4207212, whole genome shotgun sequence.
ACCESSION   DATIMH010000689 DATIMH010000000
VERSION     DATIMH010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4207212"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /gene="mutL"
                     /locus_tag="VLI90_12105"
     CDS             complement(<1..402)
                     /gene="mutL"
                     /locus_tag="VLI90_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HSV14993.1"
                     /translation="MPGNRIHQLSASLVNRIAAGEVIERPAAVVKELVENAIDAGASS
                     ILVEVEDGGRELIRIVDDGGGIAPEDLPLAFASHATSKLAGDDDLFRIATMGFRGEAL
                     ASIGAVSHARILSRTSDADAAYEIFNRGGAIS"
     gene            557..1369
                     /gene="proC"
                     /locus_tag="VLI90_12110"
     CDS             557..1369
                     /gene="proC"
                     /locus_tag="VLI90_12110"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HSV14994.1"
                     /translation="MTYELGILGAGNMAEAIVRGVLRANRLKPEQIIAADVAPARRQL
                     FAEQLKIKAVEDNAEVARQSRVILLSVKPQQSQAALAGIGGVLSPDALVISIMAGVST
                     RFIETALGGGKRWRVIRTMPNTPMLVGQGVVGVAPGQHATAADVAHAHHLFEAAADVV
                     DVTEDKIDAVTAVSGSGPAYVFFLVEQMIAAGMELGLTDAQARQLATKTALGAATMLS
                     TSNDSPAELRRKVTSPGGTTQAAITHMESQGFAQMVCDAIKAAEKRSKELGT"
BASE COUNT          312 a          510 c          537 g          271 t
ORIGIN      
        1 gctgatcgct ccgccgcggt tgaagatttc atatgcggcg tcggcatcgc tcgtgcgcga
       61 caggatgcgc gcgtgtgaga cggcgccgat gctcgccagc gcttcgccgc ggaagcccat
      121 cgtcgcgatg cggaacagat cgtcatcgcc cgccagcttg ctggtcgcgt gcgacgcgaa
      181 cgccagcggc agatcctccg gcgcgatccc gccgccgtcg tcgacgatgc ggatcaactc
      241 gcgcccgccg tcttccactt cgacgaggat gctggatgcc ccggcgtcga ttgcgttttc
      301 gaccagctcc ttgacgacgg ccgccggccg ctcgatcacc tcgccggcgg cgatgcgatt
      361 gacgagggac gcggagagct gatggatgcg attgcccggc acgacgtggg cgattatagg
      421 gacacgcgcg cgtgggggcg acgatggtgt gccgcagcgc catatcgtcc acaagcgcgc
      481 acgcgccgac cggaaggccg acggatagct atccgtgggc ttttcgcgct gttgcgatca
      541 ttctaaactg cccgtcatga cctacgaact cggcatcctg ggcgcgggca acatggccga
      601 agcgatcgtg cgcggcgtgc tgcgcgccaa tcgtctcaaa ccagaacaaa tcatcgccgc
      661 cgacgtggcg ccggcgcggc ggcaactttt cgccgagcaa ctgaagatca aagccgtcga
      721 agacaacgct gaagtcgcgc gacagtcccg cgtgatcctg ctcagcgtga aaccgcaaca
      781 gagccaggcg gccttggcgg gcatcggcgg ggtgctgtcg ccggatgcgc tggtcatctc
      841 catcatggcc ggcgtcagca cgcggtttat cgaaaccgcc ctcggcggcg gcaagcggtg
      901 gcgcgtcatc cgcacgatgc cgaacacgcc gatgctcgtc ggccagggcg tggtcggggt
      961 cgcgccggga cagcacgcga cagcggcgga tgtggcgcac gcgcatcacc tgttcgaagc
     1021 ggccgccgat gtggtcgatg tcaccgaaga caagatcgac gcggtgacgg ccgtcagcgg
     1081 gtccggccca gcgtacgttt tcttcctcgt cgagcaaatg atcgccgcgg ggatggagct
     1141 ggggttgacg gacgcgcagg cgcggcagct ggcgacgaag accgcgctgg gggcggcaac
     1201 gatgctttcc acttcaaacg attcgccggc ggaacttcgc cgcaaggtca ccagccccgg
     1261 cgggacgacg caggcggcga tcacgcacat ggaatcgcaa ggctttgctc aaatggtctg
     1321 cgacgcgata aaagcagcgg aaaaacgcag taaagaactt gggacgtgac cgcgcagaac
     1381 cgatatagcg attacacgac gttcgtggct ctggtcccct cgcccggtac tgctgaccgc
     1441 aggtctccgg gagagggtta gggtgagggt gatcgtgaaa tccgaatggc gaaatccgaa
     1501 ccctcgaatc aatgacgaat gaagaagccc gaatgacgga agtgcgattg gcgaacttct
     1561 gtcattcggt cattcggatt tggtcattca ttcggatttc ctcaccgaaa aatcaccctc
     1621 accccgaccc
//
LOCUS       DATIMH010000690         1855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3341407, whole genome shotgun sequence.
ACCESSION   DATIMH010000690 DATIMH010000000
VERSION     DATIMH010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3341407"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VLI90_12115"
     CDS             <1..660
                     /locus_tag="VLI90_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSV14995.1"
                     /translation="YNRSKILYRIAEMLEGRAPQFVAEMVQQGYAEVQAKAEVAICID
                     RLVYYAGWADKYQQVFSSVNPVASSHFNFSVLEPTGVVSILAPQDTALLGLISTIIPA
                     ITGGNTAIALAPRDLPLCAVTLAEVLATSDLPGGVVNLLTGDRAELLEHFANHMDVNA
                     IIYCGDDETERATVRTKSALNVKRAITYDRKDWSGDEAQGPYFILDTQEVKTTWHPIG
                     V"
     gene            795..1607
                     /locus_tag="VLI90_12120"
     CDS             795..1607
                     /locus_tag="VLI90_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSV14996.1"
                     /translation="MTRAHSYLCSLLILLFTATACRADVNDAPAPPGYLLHLPGIAGA
                     RSIDHSVVRGIRDGGFSGEIEIYDWTENDPGMHALLAYDRNHQEARHVADMLVQQRSA
                     HPNVPIVLMGHSGGTGIAVWALEDLPDNVQIDTLVLMSSALSPDYDLSKALRHVKGRL
                     YAFSSLADIIVLSTGTKLFGTIDGHKVEAAGRVGFTAPSGADAGQYKKLVALPYDPSW
                     LKWGDRGDHIGGMTRSFGLNVLAPLILHGTMPEVSMPTTAETPTGLGRELPR"
     gene            1604..1759
                     /locus_tag="VLI90_12125"
     CDS             1604..1759
                     /locus_tag="VLI90_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14997.1"
                     /translation="MIEQPGKRWPRWKTVLAYLVMISIASVSIWFVDRSAMRVQAQSA
                     LHVDPSR"
BASE COUNT          336 a          617 c          583 g          319 t
ORIGIN      
        1 tacaaccgtt cgaagatcct gtatcggatt gccgagatgc tcgagggccg cgcgccgcag
       61 ttcgtcgcgg agatggtgca gcaggggtac gccgaggtcc aggcgaaagc cgaagttgcg
      121 atttgcatcg accgcctcgt ctactacgcc ggctgggccg acaaatatca gcaggtcttt
      181 tccagcgtga atcccgtcgc cagctcgcac ttcaacttca gcgtgctcga gccgaccggc
      241 gtcgtctcga tcctcgcgcc gcaggacacc gcgctgctcg ggctcatctc cacaatcatc
      301 cccgccatca ccggcgggaa caccgccatc gcgctcgccc cgcgcgacct gccgctgtgc
      361 gcggtcacgc tcgccgaggt gctcgccacc agcgacctgc ccggcggcgt cgtcaatctc
      421 ttgaccggcg accgcgccga gctgctcgaa cacttcgcca accacatgga cgtgaacgcc
      481 atcatctact gcggcgacga tgaaaccgag cgcgcgacgg tgcgaaccaa gtcggcgcta
      541 aacgtgaagc gcgcgatcac ctacgaccgc aaagactggt ccggcgacga agcgcaaggc
      601 ccgtacttca tcctcgacac gcaggaagtg aagacgacgt ggcatccgat tggggtgtga
      661 atctccccca cgccgggttc ggaggtactc cgacgacccg gatgcttctc gcgcgcgaaa
      721 gagatccggg tcgtcgcgag tacgctccga acccggcgtg gcagaataag cgtcctccgc
      781 ctaccatccc gttaatgact cgcgcgcatt cgtacctctg ctcgctgctc atcctgttgt
      841 tcaccgccac cgcctgccgc gcggatgtga atgatgcgcc ggcgccgccg ggttatttgc
      901 tgcacttgcc cggcatcgcc ggggctcggt cgatcgacca ctccgtcgtc cgtggcattc
      961 gcgacggcgg tttctccggc gaaatcgaaa tctacgactg gaccgagaac gaccccggca
     1021 tgcacgcgct gctggcgtac gatcgcaatc accaggaagc ccggcacgtc gcggatatgc
     1081 tcgtgcagca gcgcagtgct caccctaatg tgcccatcgt gctcatgggc cacagcggcg
     1141 gcaccggcat cgccgtctgg gcgctggagg atctgccgga caatgtgcag atcgacacgc
     1201 tcgtgctgat gtcgtcagcg ctgtcgcccg actacgacct gagcaaagcg ctgcggcatg
     1261 tgaagggccg gctgtacgcg ttctccagtc tggcggacat tatcgtgctc agcaccggca
     1321 cgaaattgtt cggcacgatc gacggtcaca aagtcgaagc ggccggccgc gtcggtttca
     1381 ccgcgcccag cggcgcggat gccgggcaat acaagaagct cgtcgcgctg ccgtacgatc
     1441 ccagttggct caaatggggc gaccgcggcg accacatcgg cgggatgacg cgctcgttcg
     1501 gcctcaacgt gctggcgccg ctgatcctgc acggcacgat gcccgaggtc agcatgccga
     1561 cgacagcgga aaccccgaca ggcctgggcc gggagctgcc gcgatgatcg agcagccggg
     1621 gaagcggtgg ccgcggtgga agaccgtgct ggcgtatctg gtgatgatct cgatcgcgtc
     1681 ggtgtcgatc tggttcgtcg atcgcagcgc catgcgcgtg caggcgcaat cggcattaca
     1741 cgttgatcca tcgcgctagg aagacaacgg gaaaatcatt taatttagcg gcgcgtgcgc
     1801 agcaccgcgg gttggttcgc cggcggtcgt cgcaggaata gatccgcggt gctgc
//
LOCUS       DATIMH010000691         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3341530, whole genome shotgun sequence.
ACCESSION   DATIMH010000691 DATIMH010000000
VERSION     DATIMH010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3341530"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VLI90_12130"
     CDS             <1..547
                     /locus_tag="VLI90_12130"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HSV14998.1"
                     /translation="WLSLTERPSWLRLRGRQSMFSLFEQSLLARRVQSLKCTAETCVE
                     FNPTHFTQMAGLICYYDTKTFYYLRITHDEQAGKRLGIALCDDGSYNEPADAQLPIND
                     WPRAYLRAEFDRERLHFYASADGKSWQMICPVQDATRLSDDYGTGLHFTGAMVGVCAQ
                     DLGGTGATADFDYFTLTNAEG"
     gene            885..1220
                     /locus_tag="VLI90_12135"
     CDS             885..1220
                     /locus_tag="VLI90_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV14999.1"
                     /translation="MKLHKSLLIAAASVLGVSALSACESHDEGPPPPRAVVVEPGYYY
                     EPEYYDADRGYHAPIYYYYDGHHYERRDYVPHGYVPRIRQRDGAEAGGRFDNRDGELR
                     DGEHRGERR"
     gene            complement(1260..1583)
                     /locus_tag="VLI90_12140"
     CDS             complement(1260..1583)
                     /locus_tag="VLI90_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15000.1"
                     /translation="MSIAGVSLWSSDDHGDHFAPIASGTIGGRCEFGYAMNSDPTGGR
                     LACFMLDGKAGIMADGGKTWRSVVNKEWKPKLPGQWLCIGFDPQARILYASCIGNPAF
                     RLRLK"
     gene            1683..1844
                     /locus_tag="VLI90_12145"
     CDS             1683..1844
                     /locus_tag="VLI90_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15001.1"
                     /translation="MLVQPVAAAAAAGGDAASARATITRAITIAKMMLANRFVMSDLQ
                     FGATMFYFG"
     gene            complement(1832..>2026)
                     /locus_tag="VLI90_12150"
     CDS             complement(1832..>2026)
                     /locus_tag="VLI90_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15002.1"
                     /translation="QCYVTPSCCWNFISREKKVDSSFVRIVKVEQDKISDALLPVRLE
                     GETFNWETLPAKYDPNNHPK"
BASE COUNT          389 a          659 c          617 g          361 t
ORIGIN      
        1 ctggctctcg ctcacggagc gccccagctg gctgcgcctc cgcggccggc agtcgatgtt
       61 ctcgctgttc gaacaaagcc tgctcgcccg ccgcgtgcag tccctcaaat gcaccgctga
      121 gacctgcgtg gaattcaacc ccacccactt cacccagatg gccggcctga tctgctacta
      181 cgacaccaaa acgttctact acctccgcat cacccacgac gaacaagccg gcaagcgcct
      241 aggcatcgcc ctatgcgatg atggaagtta caacgaaccg gccgacgctc agctgccgat
      301 caacgactgg ccgcgggcat atctcagggc tgaattcgac cgcgagcgcc tgcacttcta
      361 cgcgtcggcc gacggtaagt cgtggcaaat gatctgcccc gtccaggacg cgacgaggct
      421 gtcagacgac tacggcaccg gcctgcactt caccggcgcg atggtgggcg tgtgcgccca
      481 ggacctgggc ggcaccggcg cgaccgcgga tttcgattat ttcacgctga cgaacgcgga
      541 ggggtgaggg aaggtagaag accacattgc attcaaacgg gcccgacatc gcacgggggt
      601 ttttattcgc gacggcaagg caactgccga gccgccgaga gcgccgaggc agacgccgag
      661 aagacacaga ggtggaaccg cagattgcgc agatttcgca gatgaagaca ctgcctcgcc
      721 gagcgggccc atggttggcg cgctgaagca ccgacgcggt ccgtacgccg tctttgcggc
      781 agcgtcatca atgctggcgc cggggctgtc acgcaacttc cggcccgcgc tttgcaaact
      841 gcgaacgcat tcggattcca ccgacctcgt acaagggcct caagatgaag cttcacaaat
      901 ccctgctcat cgcggccgcg tcagtcctgg gcgtgagcgc cttgagcgca tgcgagtcac
      961 acgatgaagg ccccccgccg ccgcgcgcgg tggttgtcga acccgggtat tactacgagc
     1021 cggagtatta cgacgccgac cgcgggtatc acgcgccgat ttattattac tacgacgggc
     1081 atcactacga gcgtcgcgat tacgtgccgc acggctacgt cccgcgcatt cgccagcgcg
     1141 acggtgcgga agccggggga cggttcgaca accgcgacgg cgagttgcgc gacggcgagc
     1201 accgcggcga gcgaagatga tcgctggcgc gacaacggac gcgctcgcga cgatgtgaat
     1261 cacttgagtc gcagtcgaaa cgccggattg ccaatgcagg atgcgtagag gatccgcgcc
     1321 tgtggatcga agccgatgca cagccattgg cccggcagct ttggcttcca ttctttattg
     1381 acgacgctgc gccacgtctt gccgccatcc gccatgatgc cggctttgcc atcgagcatg
     1441 aaacacgcca gccgcccacc ggtcggatcg ctgttcatcg cgtaaccaaa ctcgcagcgc
     1501 ccgccgatgg tgccgctggc gatcggtgcg aagtgatcgc cgtgatcgtc gctgctccac
     1561 agactgacgc ccgcgatcga caaatatacg cggccgctga cgcggtcgca ggcaatgccg
     1621 ccgctctggc ccagggaatc ttcgtgccgg tcgaggtcag ctgctgcagg agcgcatcgg
     1681 agatgttggt ccagccggtc gcggcggcgg cggcggcggg tggtgacgct gcttctgctc
     1741 gcgcgacgat cacccgcgcc atcaccatcg caaagatgat gctcgccaat cgcttcgtca
     1801 tgagcgatct ccaattcggc gccacgatgt tttacttcgg gtgattgttc ggatcgtact
     1861 tcgcgggcag tgtttcccag ttgaacgttt cgccttccag tcgaaccggc aacagcgcat
     1921 cggagatctt gtcctgttcc accttcacga tccggacgaa gctggaatcg accttcttct
     1981 cgcgactgat gaagttccag cagcagctgg gggtcacgta acactg
//
LOCUS       DATIMH010000692         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2474836, whole genome shotgun sequence.
ACCESSION   DATIMH010000692 DATIMH010000000
VERSION     DATIMH010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474836"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1157
                     /locus_tag="VLI90_12155"
     CDS             75..1157
                     /locus_tag="VLI90_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSV15003.1"
                     /translation="MRAIVTGQVGVDKGPYLEAVKATAKKAGHDLEVFHVGKMMYKEA
                     PDVPAGRILNLPITRLNTLRRAVFKEILRRAEEHEHVLVNTHATFRWRHGLFAAFDFD
                     QLKEFKADLYLTLLDNAESVHQRLMRDHDIDHTLKDIMVWREEELLATEILANITRGY
                     GHFFMISRGRNIPTTQTIFRLMFEAHKKKVYPSFPMSHVMDLPDTLAEIDRFRAKLAE
                     DFITFDPGDVDEFVLHTTALKAMAEGQTTMEVEAAEGKVTLKTADVAQISGDIMGQIY
                     ARDFKMVDQSDMIVSLVPELPNGKPGLSSGVERELHHAFEGGKEVYVIWACRGTPSPF
                     ITETATRVFKNVDEAMEHFRQKGYVK"
     gene            1567..2634
                     /locus_tag="VLI90_12160"
     CDS             1567..2634
                     /locus_tag="VLI90_12160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV15004.1"
                     /translation="MKSEIAAASPRVGVVAIGRNEGERLRACLVSARRDCSSVVYVDS
                     GSSDESVALARSLGCHVVELDLATPFTAARARNEGFAFLKQIDSDVQFVQFVDGDCEI
                     VVGWIAKATAELAARSRAAIVCGRRRERFPDASVYNRLCDMEWNTPIGQAKACGGDAL
                     VRVSAFEEVGGYDGSVIAGEEPEMCVRLRERGWTIHRIDAEMTLHDAAMTRASQWWRR
                     NVRGGHAYAQGFAMHGAPPENCYRRQLRSIAFWGFAPLIATAVMIVALLIARPMWVWV
                     GFFPMLGYAALAAKSGFWRFRRGDRAKDAAMYGVAVTLAKFPQAVGVRKFRRAQKRGI
                     RNTIIEYKGPAHPRSVQGQQS"
     gene            2631..>4643
                     /locus_tag="VLI90_12165"
     CDS             2631..>4643
                     /locus_tag="VLI90_12165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV15005.1"
                     /translation="MTQQVESINAAAVQPTAGTAHPAPHKRPALMPSDPLLALQSLLR
                     LPVPIVIVAERAAVDEGGSIEEMEVDALRARGWAVRMVTLCDVHQMAIGIGRELLSHP
                     LDTLRTFAAAARDALAPGERTSFMNRLRALGSGMAGICLANQVREHGHARHVHCQSSR
                     AATAGMYAASQLGVRFSFSAQGNEDGTLLKRKLERAAFVACANRSQREEYRKISPADE
                     TFYPLVHRGVNMGLLDADDLNGRHFTTQRAGIVRVLTLAPADVAIRAMNRFGETTGRF
                     WQLTIAGNDDRRAARLIAGELGVSDRVDFVDADRATELRDLLRGSDLFVVSADRNASQ
                     TNALTMALSIGIPVIAPDAPAMRELLLAKDGAAGVLVPSDDPAALAGAIEKLSNEPSL
                     RRSLIAGGRGVMAREFDFELNVSRLERMLEATVRRTDAGSNDAAAAGGAKSRRYALIT
                     PCRDEAKYARRTLDSVTSQSIPPAVWVIVDDGSKDETPHILAEYAARFRYIKIVTRAD
                     RGDRKLGGGVIDAFYAGYETINPDDYDYVCKLDLDLDLPRRYFETLMERMEQSPRIGT
                     CSGKPYFTRDGQQVSEKCGDEASVGMVKFYRTECFKQIGGFVRELMWDGIDCHQCRMN
                     GWLAVSWDDPAINFEHLRPMGTSHKNWWTGRARHGSGQYFMGTGPIY"
BASE COUNT          865 a         1333 c         1589 g          856 t
ORIGIN      
        1 aacgattccg ccggtgctgc atgcggtgta caatgccgcg tcgggttggg gtccgcggaa
       61 tttgtggagc acacatgcga gcgatcgtta cgggacaagt cggcgtcgat aagggtccat
      121 atctcgaagc cgtcaaagcc acggcgaaga aggcggggca tgacctggag gtctttcacg
      181 tcggcaagat gatgtacaaa gaagcgcccg atgttcccgc ggggcgcatc ttgaatctgc
      241 cgatcacccg gctcaacacg ctgcgccggg ccgtcttcaa ggaaatcctc cgccgcgccg
      301 aagagcacga acacgtgctg gtaaatacgc acgccacctt ccgctggcgg cacgggctgt
      361 tcgccgcgtt cgacttcgac cagctcaagg agttcaaggc agacctgtac ctcacgctgc
      421 tggacaacgc cgagagcgtt caccagcgct tgatgcgcga ccacgacatc gaccacacgc
      481 tcaaagacat catggtctgg cgcgaggaag agctgctggc gacggaaatt ttggcaaaca
      541 tcacgcgcgg ctacgggcac ttcttcatga tcagccgcgg gcgaaacatc ccgacgacgc
      601 agacgatttt tcggctgatg ttcgaagcgc acaagaagaa agtttatcca tcgttcccga
      661 tgagccacgt gatggacttg cccgatacgc tggccgagat cgatcgcttt cgcgccaagc
      721 tggcggagga tttcatcacc ttcgaccccg gcgacgtgga tgaattcgtt ctgcacacga
      781 ccgcgctgaa ggcaatggcc gaaggacaga cgacgatgga agtcgaagcc gccgagggca
      841 aggtgacgct gaagacggcg gacgtggcgc agatcagcgg cgacatcatg ggtcagattt
      901 acgcgcgcga tttcaagatg gtagatcagt ccgacatgat cgtgtcgctc gttccggaac
      961 tgccgaatgg caagcctggc ttaagcagcg gcgtggagcg cgagctgcac cacgcgttcg
     1021 aaggcgggaa agaagtctac gtcatctggg cctgccgcgg cacgccaagc ccgttcatca
     1081 ccgagaccgc gacgcgggtg tttaagaatg tggatgaggc gatggagcac tttcggcaga
     1141 aggggtatgt gaagtgaagc cgatgcacgc tcaccgcgac acgggcctcc ggcgcgtgcg
     1201 caatgcgttg gtacctctga aacggtaggt tctacaactt cgaatgtagc tcgcgcgggc
     1261 cggaggcccg tgtcacggtg agcgctaacg ttcgcgcata ccgcacatcc gaaatagcgt
     1321 cgcgctttca tgcgatgggt aatggctaac gcaacggctt ccgacgccgc tggtctactg
     1381 atatcccacc acgccgggtt cggagcgtac tcgcgacgaa gcgagattct tctggcgcgc
     1441 gcgccatgtc ataaccaggt ccgatccttc ccgcttattt cattttctct cggtacgagc
     1501 ttgagccgat agagattctc agctgaatgc ttatcgtacc gggcgagtat ggcgtctgat
     1561 gttcaagtga aatcggaaat cgccgcggca tcgccgcgag ttggcgtggt ggcgatcggc
     1621 cgcaacgaag gcgagcggct gcgggcgtgc ctcgtgtcgg cgcggcggga ctgctcgagc
     1681 gtcgtatatg tggactcggg gtccagcgac gaatcggtcg cgctcgcgcg gtcgctggga
     1741 tgtcatgttg tggagctgga cctggccacg ccattcacgg cggctcgggc tcgcaatgag
     1801 gggttcgcat ttctcaagca gattgattcc gatgttcagt tcgtgcagtt cgtcgatggc
     1861 gattgtgaga tcgtcgtggg atggatcgcc aaagcgacgg cggagctcgc ggctcggtcg
     1921 cgggcggcga tcgtctgtgg tcggcggcgc gagcgctttc cggatgcttc ggtttacaac
     1981 cggctgtgcg acatggagtg gaacacgccg atcggccagg cgaaggcgtg cggtggcgat
     2041 gcgctggtgc gcgtgtcggc gtttgaggaa gtcggtggct acgacggcag cgtgatcgcc
     2101 ggcgaggaac cggagatgtg cgtccgcctg cgcgagcgcg ggtggacgat ccatcggatc
     2161 gacgcggaga tgacgctgca cgatgcggcg atgacgcgcg catcgcaatg gtggcggcgg
     2221 aacgtccgtg gaggacatgc gtacgcgcag ggcttcgcga tgcacggggc gccgccggag
     2281 aactgctacc gccgccagct gcgcagtatc gcgttctggg gatttgcccc gctgatcgct
     2341 acagccgtca tgatcgttgc gctgctcatc gcgcggccga tgtgggtttg ggtcggcttt
     2401 ttcccgatgc tgggatacgc ggcgctggcg gcgaagtcgg gtttttggcg atttcgccgg
     2461 ggagatcgag cgaaggacgc ggctatgtat ggcgtggcgg tgactttggc gaagttcccg
     2521 caggcggtgg gtgtgcgaaa gtttcgacgt gcccaaaagc ggggtatccg aaataccatc
     2581 attgagtaca aaggcccggc gcatccacgt tctgtacaag ggcagcagtc atgacccagc
     2641 aggttgagtc catcaatgca gcagcggtgc agccaaccgc ggggacggcg catccggctc
     2701 cccacaagcg accggcgttg atgccgtcgg atccgctgct ggcgctgcag tcgctgttgc
     2761 gattgccggt gccgatcgtg atcgtcgctg agcgagccgc cgtcgatgaa ggtggctcga
     2821 tcgaggagat ggaagtcgat gctctgcgag cgcgcgggtg ggccgtgcgc atggtgacgc
     2881 tgtgcgatgt acaccagatg gcgatcggca tcgggcgcga gttgctgagc catccgctgg
     2941 atacgctgcg gacgttcgct gccgctgcgc gcgatgcgct ggcgccgggc gagcgtacgt
     3001 cgttcatgaa tcggctgcgc gcgctgggga gcgggatggc gggcatctgc ctggcgaacc
     3061 aggtgcgcga gcacgggcat gcgcgacacg tgcattgcca atcgagccgc gcggcgacgg
     3121 ccgggatgta cgcggcgtcg cagctgggcg tgcgcttcag cttctcagcc cagggcaatg
     3181 aagacggcac gctgctcaag cgcaaactgg agcgggcggc gttcgtcgcg tgcgccaacc
     3241 gatcgcagcg cgaagagtat cgaaagattt ctccggcaga tgagacgttt tatcccctcg
     3301 tccaccgcgg tgtgaacatg ggtctgctag atgccgatga cctgaatggc cggcacttca
     3361 cgacgcagcg ggcgggcatc gtccgcgtgc tgacgttggc accggccgat gtcgcgatcc
     3421 gcgcgatgaa ccgcttcggc gaaacgaccg ggcggttctg gcaattgacg atcgccggca
     3481 acgacgatcg ccgagcggca cggttgatcg ccggtgaact cggcgtcagc gaccgcgtcg
     3541 atttcgtcga tgccgatcgc gcaaccgagc tgcgcgatct gctccgcggc agcgacctct
     3601 tcgtagtgtc tgccgatcgc aacgcttcgc aaacaaacgc gctgacgatg gcgttgtcta
     3661 ttggcatacc cgtgatcgcc cctgatgcgc cggctatgcg cgaactgctg cttgccaagg
     3721 acggggcggc gggtgtgttg gtcccgagtg acgaccccgc ggcactggcg ggggcgatcg
     3781 aaaaattgtc aaatgaacct tcgcttcgcc gatcgctgat cgccggcggg cgcggcgtga
     3841 tggcaaggga gtttgatttc gagttgaacg tctcgaggtt ggaacgaatg ctggaagcga
     3901 cggtcagacg aacggatgcg ggctcaaacg acgcggcggc ggccggcggc gccaaatcgc
     3961 ggcgttacgc gctgatcacc ccctgccgcg acgaggcgaa gtacgcccgg cgtacgctcg
     4021 attcggtgac gtcgcagtcg attccccccg cggtgtgggt catcgttgat gacggctcga
     4081 aggacgagac gccgcacatc ctggctgagt acgctgctcg ctttcgatac atcaagatcg
     4141 tcacccgcgc cgatcgcggc gatcgcaagc tgggcggcgg tgtgatcgac gcgttttacg
     4201 ccgggtacga gacgatcaat cccgacgatt acgattacgt ctgcaagctc gatttggact
     4261 tggacctgcc ccgccgctac ttcgagacgt tgatggagcg gatggagcag agcccgcgca
     4321 tcggcacgtg cagcggcaag ccatacttca cgcgcgatgg ccagcaggtc agcgagaagt
     4381 gcggcgacga agcgtcggtg ggcatggtga agttctatcg cacggaatgc ttcaaacaga
     4441 tcggcgggtt cgtgcgcgag ttgatgtggg acggcatcga ctgccaccag tgccggatga
     4501 acggctggct cgccgtcagc tgggatgatc cggcgatcaa cttcgaacac ctccggccga
     4561 tgggcacgag ccacaagaac tggtggaccg gccgggcacg gcacggttcc ggccagtact
     4621 ttatgggaac cgggcccatc tat
//
LOCUS       DATIMH010000693         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_165796, whole genome shotgun sequence.
ACCESSION   DATIMH010000693 DATIMH010000000
VERSION     DATIMH010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165796"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1945)
                     /gene="yicI"
                     /locus_tag="VLI90_12170"
     CDS             complement(<1..1945)
                     /gene="yicI"
                     /locus_tag="VLI90_12170"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: protein motif:HMM:NF007940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HSV15006.1"
                     /translation="MKFCDGAWLWVKGVDAHCMQRVSGYRVDDNASTLVAWAVDRRGK
                     EGADRFEGTILELRVTSPMSDVIRLQVRHHRPADGPRFDLDYNLSASGVRIDDGEQEL
                     RFTSGRLSLRLKKGPPFDLRFEDEHGQLVTGGVKEPLAFARVEGAGEFLTQRLSLGVG
                     ECIYGLGERFGPLVKNGQTVTIWNQDGGTISDQSYKSVPFYLSSRGYGVLVNTPGKVE
                     FEIATERVSQVQFSVQEQSLDYYVFLGPEPKDVLEKYTRLSGRPAMPPAWSFGLWLST
                     SFTTKYDEQTVNEFVDGMAQRQIPLSVMHFDCFWMKERHWCDFEWDRKAFPDPRGMIE
                     RLKAKGLRVCLWINPYISQLSKLFDEGRSGSFFLKRADGSVYQIDGWQPGMAFVDFTN
                     PAAVAWYTSKLRALLEMGIDCFKTDFGERIPDDAVYHNGADPKRMHNYYSYLYNQAVF
                     KLLEEHHGKGNAIVFARSGTAGSQKFPLHWGGDCEATFESMAEDLRGGLSFCMSGAAF
                     WSHDIGGFTGKADPGIYKRWVAFGLLSTHSRLHGSESYRVPWIFDEESVGVMRRFVNL
                     KNALFPYLFAAARDANEHGWPVMRAMALEFPDDPGCRHLDRQYMLGSSLLVAPIFSHD
                     GVAEYYVPQGRWTNLLTGKTVDGG"
     gene            complement(2059..>2839)
                     /locus_tag="VLI90_12175"
     CDS             complement(2059..>2839)
                     /locus_tag="VLI90_12175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV15007.1"
                     /translation="LTLNAGGSMASANYTVAATATLNANGALASTANVTANGAVNFGG
                     TTGGTARTQALGTLAIGSGVTVSITHSAFAFTPTILKPNTTTFADSTAKIDLSDNTFI
                     TNGTAAGALGLIQAGQIFSSEIADPNKAIGYIDLSGADAGNYETRYTLKGDTNLDGAV
                     GVGDLGALATSYGVTGGMSWANGDFNQDHNVNVADLGALATNYGTQLSTSSAVATSLA
                     IVASGGGGGGGAAVPEPASVSLLGIGALTLASGRRRRRLAD"
BASE COUNT          518 a          926 c          865 g          530 t
ORIGIN      
        1 caccgccatc aaccgttttt ccggtgagca gattcgtcca tcgcccctgc ggcacgtagt
       61 actcggccac gccatcgtgc gaaaagatcg gcgccaccag cagcgatgaa ccgagcatgt
      121 actgccggtc gagatgcctg caacccggat catcggggaa ctccagcgcc atcgcccgca
      181 tcaccggcca gccatgttcg ttcgcgtcgc gcgccgcggc gaacaggtat gggaacagcg
      241 catttttaag gttcacgaat cgccgcatca cgccgacgga ttcttcgtcg aagatccacg
      301 gcacgcggta agattctgag ccgtgcaatc gactgtgcgt cgagagcagt ccgaaggcta
      361 cccatcgttt gtagatgccc ggatcggcct tgccggtgaa cccgccgatg tcgtggctcc
      421 agaacgccgc gccggacatg cagaagctca agcccccgcg caggtcctcg gccatcgatt
      481 cgaacgtcgc ttcgcaatcg ccgccccagt gcagcggaaa cttttgcgag cccgcggtgc
      541 ccgagcgcgc gaagacgatc gcgttgccct tgccgtggtg ctcctcgagc agcttgaaga
      601 ccgcctggtt gtacaggtac gaataatagt tgtgcatccg cttcggatct gcgccgttgt
      661 ggtagacggc atcgtcggga atgcgctcgc cgaagtcggt cttgaagcag tcgatgccca
      721 tctcgagcag cgcccgcagc ttggacgtgt accacgcgac ggcggcgggg ttggtgaagt
      781 cgacgaacgc catgcccggc tgccagccgt cgatctggta cacgctgccg tcggcgcgct
      841 taagaaagaa gctgcccgat cgaccctcat cgaacagctt cgagagttgt gagatgtacg
      901 ggttgatcca caggcagacg cgcaggccct tggctttgag acgctcgatc atgccgcgcg
      961 gatcggggaa tgcttttcgg tcccactcga agtcgcacca gtgccgctcc ttcatccaga
     1021 agcagtcgaa gtgcatcacg ctgagcggga tttgccgctg cgccatgccg tcgacgaact
     1081 cgttcacggt ttgctcgtcg tacttcgtgg tgaaggacgt gctgagccaa agcccgaacg
     1141 accacgccgg cggcatggcg ggacggccgc tgagacgcgt gtatttctcc agtacgtcct
     1201 tcggctcggg gccgaggaag acgtagtaat ccagcgactg ttcctgcacg ctgaactgca
     1261 cctgcgagac gcgttcggtg gcgatttcga attcgacttt ccccggcgtg ttcaccagca
     1321 cgccgtaccc gcggctggac aaatagaacg gcacgctctt gtacgactga tcgctgatcg
     1381 tgccgccgtc ctggttccag atggtgacgg tctgcccgtt cttcaccagg gggccgaatc
     1441 gttcgcccag cccgtagatg cattcaccga cgcccagcga aagacgctgc gtgaggaatt
     1501 cgccggcgcc ttcgacgcgg gcgaaggcga gcggttcttt gacgccgccg gtgaccagct
     1561 ggccatgctc gtcttcaaat cgcagatcga acggcggacc ttttttgagc cgcagcgaca
     1621 accgcccgct ggtgaaacgc agttcctgct cgccatcgtc gatgcgaacc ccactggcgg
     1681 acaagttgta atccagatcg aaccgcggtc cgtcggccgg ccgatgatga cggacctgga
     1741 gccggatgac gtcgctcatc ggcgaggtca cccgtagctc gagaatggtt ccttcgaacc
     1801 gatccgcgcc ttctttgccc cgacgatcga cggcccaggc aaccagcgtt gacgcgttgt
     1861 cgtccacgcg ataaccgctg acgcgctgca tgcagtgcgc atcgacaccc ttcacccaca
     1921 accaggcacc gtcacaaaac ttcacggcac aaactcctgt cgctgtccat ctcgatgttg
     1981 cgtctgaact atctctgaat atcaaggcgg cggcccgccg gtcagggacg ggccgccgca
     2041 gcggaggggg agtcgagatc agtcggctaa ccggcgccga cggcgaccgc tcgccaacgt
     2101 caacgcgccg atgccgagca gactgaccga agcaggttcg ggcaccgcag cgccaccacc
     2161 accaccgcca ccgctggcaa cgatcgccag gctggtcgcg acggccgagc tggtgctcaa
     2221 ctgcgtacca taattcgtcg ccagcgcacc cagatccgcg acgttgacgt tatggtcctg
     2281 gttgaaatcg ccgttggccc agctcatccc accggtgacg ccgtagcttg tggccaacgc
     2341 gccaagatcg cccacgccca ccgcgccatc cagatttgtg tcgcctttga gcgtgtagcg
     2401 agtctcataa ttgcccgcgt ccgcgccgct caggtcgata tagccgatcg ccttgttggg
     2461 atcagcgatt tcggaactga agatctgccc ggcctgaatc agccccagcg cgccggcggc
     2521 ggtgccgttg gtgatgaagg tgttatcaga cagatcgatc ttcgcggtgc tgtcagcgaa
     2581 cgtcgtggtg ttcggcttca ggatcgtcgg tgtaaacgcg aacgccgagt gcgtgatgct
     2641 gactgtgaca ccgctgccga tcgccagcgt gcccagcgcc tgcgttcgcg cggtgccgcc
     2701 ggtggttcca ccgaagttca ccgcgccgtt ggcggtgacg ttcgcggtcg aagccagcgc
     2761 gccgttggcg ttcaacgtgg cggtcgcggc aaccgtgtag ttcgcgctgg ccatcgagcc
     2821 cccggcgttc aacgtcagg
//
LOCUS       DATIMH010000694         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1034162, whole genome shotgun sequence.
ACCESSION   DATIMH010000694 DATIMH010000000
VERSION     DATIMH010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034162"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VLI90_12180"
     CDS             <1..507
                     /locus_tag="VLI90_12180"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSV15008.1"
                     /translation="MPVLTTRCSNNYGPYHFPEKLIPLFVTNLIEGKKVPLYGDGLNV
                     RDWLYVEDHCDAIWTVLNKGTFGEAYNVGGNNEMTNRRITEIVLSEMGKSWDESVQYV
                     KDRPGHDRRYAIDAGKMQRELGWRPKHKFEEAIKTTIQWYKDHQDWWRSIKSGDYLKY
                     YETQYVNR"
     gene            558..1052
                     /locus_tag="VLI90_12185"
     CDS             558..1052
                     /locus_tag="VLI90_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV15009.1"
                     /translation="MPSPQVTTRRATEADVPHILRFIRALAEYEKLEHQMVATEEGLR
                     HHLFGPHPAAEAMIGAIDTQPVGYALFFTTFSTFLAKPSIWLEDLFVLPDHRSRGVGR
                     ALLRAVADIAIERDCGRLEWSVLDWNQPAIDFYHRAGAKILNDWRICRVTGEALQKMG
                     ASHA"
     gene            1045..1929
                     /gene="rfbD"
                     /locus_tag="VLI90_12190"
     CDS             1045..1929
                     /gene="rfbD"
                     /locus_tag="VLI90_12190"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HSV15010.1"
                     /translation="MPDVSDSIVITGGGGMLGHALADALAHRGRQAVALTRAECDIAN
                     DDDIHRIFERPPTLLLNCAAHTKVDLCEQESALADAINGTAVGKIAKLAREHGTYLVH
                     VSTDFVFEGHGTHPYKIDDPVKPLSAYGRSKLLGETELQKNAPRHWLIVRTAWVYGRY
                     GANFPRTMVSAARAGKPLSVVRDQVGSPTYTVDLAEAILALVDARANGIVHVTNAGET
                     NWFEFAKAALETFGIDHPVAAITAADWQKLRPNTAARPSYSVLDLRPYEGITGKTMRP
                     WREALSDFRKEVDRAGAF"
     gene            2181..2597
                     /locus_tag="VLI90_12195"
     CDS             2181..2597
                     /locus_tag="VLI90_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15011.1"
                     /translation="MRPRATAFVVVFVIAAAAAWVVQRHNDLPRYGEPSFTVINGSSM
                     KPHRLRGDETIATALSTLPDNDTGRPTTQPDTLLLVRRGPDGLGRQRIDYDASMHLVD
                     PAKDQSLRNGDQLIVAPKPTPEGLARPAGPGMPVTN"
     gene            complement(2717..>2976)
                     /locus_tag="VLI90_12200"
     CDS             complement(2717..>2976)
                     /locus_tag="VLI90_12200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HSV15012.1"
                     /translation="PEGVPTERELKPFGGAVQGQNDFKARGWSGPKPPMGHGVHHYHF
                     QLFALDQQLGLGPDALLDELANAMRGHVIAEGEIVGTYERK"
BASE COUNT          578 a         1007 c          893 g          498 t
ORIGIN      
        1 atgccggtgc tgaccacccg ctgcagcaac aactacggtc cgtaccactt ccccgagaag
       61 ctgatccccc tgttcgtcac caacctgatc gaaggcaaga aggtcccgct ctacggcgac
      121 gggctgaacg tgcgggactg gctctacgtc gaggaccact gcgacgcgat ctggacggtg
      181 ctgaacaagg ggacgttcgg ggaggcgtac aacgtcggag ggaacaacga gatgaccaac
      241 cggcggatca cggagatcgt gctgtcggag atgggcaagt cgtgggacga gtcggtgcag
      301 tacgtgaagg accgccccgg ccacgaccgc cgctacgcca tcgacgccgg caaaatgcag
      361 cgcgagctgg gctggcggcc caagcacaag tttgaggaag cgatcaagac gacgatccag
      421 tggtacaagg accaccagga ctggtggcga agcatcaaga gtggcgatta tctaaaatac
      481 tacgaaacgc agtacgtgaa tcgctaagca cgaacgcacg cgcggcgtgg tcgcttcagc
      541 gaccatggaa acgcccaatg ccttcccccc aagtcaccac ccgccgcgcc accgaagcgg
      601 atgtcccgca catcctccgc ttcatccgcg cgctcgcgga atacgaaaaa ctcgagcacc
      661 agatggtagc caccgaagaa ggcctgcgtc atcacctctt cggcccgcac cccgcggccg
      721 aggcgatgat cggcgcgatc gacactcagc ctgtcggcta cgccttgttc ttcacgacct
      781 tctcaacctt cctcgccaag cccagtatct ggcttgaaga cctgttcgtc ctaccggacc
      841 accgcagccg cggcgtcggc agagcgctgc tccgcgccgt cgccgacatc gccatcgagc
      901 gcgattgcgg tcgcctggaa tggtccgtgc tcgattggaa ccagccggcg atcgactttt
      961 accaccgcgc tggcgcgaag atattgaatg actggcgcat ctgccgcgtc accggcgagg
     1021 cgcttcaaaa gatgggtgca tcacatgcct gacgtttccg attccatcgt catcaccggc
     1081 ggcgggggca tgctcgggca cgcgctggcg gacgccctcg cccaccgcgg ccggcaggcc
     1141 gtggcgctga cgcgcgccga gtgtgacatc gccaacgacg acgacatcca ccgcatcttc
     1201 gaacgcccgc cgacgctgct gctgaactgc gcggctcaca ccaaggtgga cctgtgcgag
     1261 caggagtcgg cgttggccga cgcgatcaac ggcactgccg tcggcaaaat cgcgaagctc
     1321 gcccgcgagc acggcacgta tctcgtccac gtcagcaccg acttcgtctt cgaaggccat
     1381 ggcactcatc cgtacaagat cgacgatccg gtcaaacccc tctcggccta cggccgatcg
     1441 aaactgctcg gcgaaacgga gctgcagaag aacgccccgc ggcactggct gatcgtccgc
     1501 actgcctggg tgtatggccg ctacggcgca aactttcccc gaacgatggt cagcgccgcc
     1561 cgcgcgggca agccgctgag cgtcgtccgc gatcaagtcg gctcgccgac gtacacggtc
     1621 gatctggccg aggcgatttt ggcgctggtt gacgcccgcg cgaatggcat cgtccacgtg
     1681 accaacgccg gtgaaaccaa ctggtttgaa ttcgccaaag cggcgctgga gacgtttggg
     1741 atcgatcatc ccgtcgccgc catcaccgcc gccgactggc agaaactccg ccccaacact
     1801 gccgcccggc cgagttacag cgtgctggat ttgaggccgt acgaagggat tacggggaag
     1861 acgatgcgcc cgtggcgcga ggcgctgagc gattttcgca aggaagtcga tcgcgctggc
     1921 gcgttttaat cagcgctttg atagccgctt aatagccgca ggcgacagcc agcgggatct
     1981 cccgatcacc aaccgggtca cgaggtcgag cgcccccgct ggctgtcgcc tgcggctatt
     2041 aaatgtccac tcgcacaacc ccgcgtccgt cgcgccgctg acgtgaccca ctgcattttt
     2101 cgcccgcctt gcaaacgtga tgtatactgg attcaccggc gttcgatcgc cgtcgccctc
     2161 gcctctgtcc aggaaacatt atgagaccca gggccaccgc gttcgtggtg gtgttcgtga
     2221 tcgccgccgc ggcggcgtgg gtggtgcagc gccacaacga tctgccgcgc tacggggaac
     2281 cgtcgttcac cgtcatcaac ggctcgtcga tgaagccgca ccgccttcgc ggcgacgaga
     2341 cgatcgcgac cgcgctctcc accctccccg acaacgacac cggcagaccg accacgcagc
     2401 ctgacacgct gctgctggtc cgccgcgggc ccgacgggct tgggcggcaa cgcatcgact
     2461 acgatgcgtc gatgcacctg gtcgatccgg cgaaggatca atcactgcgg aacggcgacc
     2521 agctgatcgt cgcgcccaag cccaccccag aaggactagc gcgtcccgca ggaccgggga
     2581 tgccggtgac gaattaaacg cgaacaattt tcctccgcgt agcggtagcc ccgaaggggc
     2641 acgtgtgttc cctttgagcg agaaaacacg tgccccttcg gggctaccgc tacgcagggg
     2701 aattgtatca tcgcgattat tttcgctcgt acgtcccgac gatctccccc tccgcgatca
     2761 cgtggccgcg catcgcgttc gccagttcat ccaacagcgc atcgggaccc aggcccagtt
     2821 gctgatcgag cgcgaatagt tggaagtgat agtgatgcac gccatggccc atcggcggtt
     2881 tcggcccgct ccagccgcgg gctttaaaat cgttctggcc ctgcaccgcg ccgccgaatg
     2941 gtttcagctc gcgttccgtt ggcacgcctt cgggta
//
LOCUS       DATIMH010000695         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_746426, whole genome shotgun sequence.
ACCESSION   DATIMH010000695 DATIMH010000000
VERSION     DATIMH010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746426"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VLI90_12205"
     CDS             complement(<1..1068)
                     /locus_tag="VLI90_12205"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HSV15013.1"
                     /translation="MTITSASFLAAVMIVAGVIFFLPRGLPRQILLALCNAAFLSLSI
                     PNLSSAIAMAVFVLSGFFVADLVRRIPNHRARPYLLASYLVLLLAAFVTLKEYQFLNF
                     LIPPGTVSRWISLVGLSYILFRQIHYVVDVAEGQIERASLWGYLNYQFDLFALYSGPI
                     QRYQHFTESWGALAPILDDTHLRLRALGRLMLGIIKVAAISTLMLKIADHAASRQLHI
                     RSPRDWVAFAALFYAYPAYIYFNFSGYCDVVIAAAAFVGIDLPENFNHPYLARNAIDF
                     WGRWHMTLTHWIRDYVFTPLYKKGVETNIIRPQRLSYACYFIALFLAGLWHGSTANFA
                     IFGLLHGAGVSVTKMWEEHIIR"
     gene            complement(1065..1337)
                     /locus_tag="VLI90_12210"
     CDS             complement(1065..1337)
                     /locus_tag="VLI90_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSV15014.1"
                     /translation="MSSISPESIREHTQTVFRNVFNDDEIVLSDAMTADDVDGWDSLT
                     HINLIIAVERQFGIKFATAEISGLKADDQNIGTFLKLIQKKLDAKR"
     gene            complement(1330..>2679)
                     /locus_tag="VLI90_12215"
     CDS             complement(1330..>2679)
                     /locus_tag="VLI90_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HSV15015.1"
                     /translation="PPWRVMHNHEMRHPAGLARFISKLNHAMQDAAPSYVTIHDVDHL
                     AASAGRWAWGDDRFFYHAKLPCAPEFLAGYAHSVASIIAAQAGASKKCLVLDLDNTLW
                     GGVIGDDGIGGIRLGQGDAEGEAFQAFQHYCNDLARRGVILAVCSKNTEAVAREAFEK
                     HPEMVLRIEDIAAFVANWDDKARNLRLIARTLEIGLDSLVFVDDNPVERALVRQLAPE
                     VAVPELPEDPAGYIQAIDRHAYFQTVSVGAEDLQRTEMYRANAKRQQAEVSSGGVEDF
                     LRSLDMRARIDPINSANLERVAQLVARSNQFNLTTRRHSAAALMKMAGSPDYLTLTVS
                     LADRFGDNGLISVVIGRVEADELVIDTWLMSCRVLKRGVESFVRNVLCRMARQRGLTA
                     LRGEYIPTPKNALVIEHYANLGFAKISGEVGQQSWWQLPLSAAAEWPTFIQERTAHE"
BASE COUNT          519 a          789 c          891 g          480 t
ORIGIN      
        1 gcggatgatg tgttcttccc acattttcgt caccgaaacg ccggcgccgt gcagcaggcc
       61 gaagattgcg aagttcgcgg tcgatccatg ccacagcccg gccaggaaca gcgcgatgaa
      121 gtagcacgcg tagctcaacc gctgcggtcg aatgatgttc gtctccacgc ctttcttgta
      181 cagcggcgtg aagacgtagt cgcggatcca gtgcgtgagc gtcatgtgcc aacgacccca
      241 gaagtcgatc gcgttgcgcg ccaggtacgg gtgattgaag ttctccggca ggtcgatgcc
      301 gacaaacgcg gcggcggcga tgacgacgtc gcagtagccg gaaaaattga aatagatgta
      361 cgcggggtaa gcgtaaaaca acgcggcgaa ggcgacccag tcgcgcggtg agcggatgtg
      421 cagctgccgc gacgcggcat gatcggcgat cttcaacatc agcgtgctga ttgccgcgac
      481 cttgatgatc cccagcatca gccggcccag cgcgcgaagg cgaaggtgcg tgtcgtccaa
      541 gatcggggcg agcgctcccc agctttcagt gaagtgctga taccgctgga tcggaccgga
      601 gtagagcgcg aacagatcga attgataatt gagatacccc cacagcgacg cgcgttcgat
      661 ctgtccttcg gccacatcaa cgacgtaatg aatttgccgg aacagaatgt agctcaggcc
      721 cacgagcgag atccatcgcg acaccgtgcc gggtgggatc aggaagttga ggaactgata
      781 ttcctttagc gtgacgaacg cggccagcag caggaccagg tacgacgcca gcaggtaggg
      841 tcgggcgcgg tggttgggaa tccgccgcac aagatcggcg acgaaaaatc cgctgagcac
      901 gaagaccgcc atcgcgatgg cgctgctcaa gttggggatc gacagcgaca gaaacgcggc
      961 gttgcacagc gccagcagga tttgccgggg caacccgcgc gggagaaaaa agatgacccc
     1021 cgccacgatc atcaccgccg ccagaaacga tgcgctggtg atggtcatcg cttggcgtcg
     1081 agtttcttct ggatcagctt caagaacgtc ccgatgttct gatcgtcggc cttgagcccg
     1141 gagatttcgg cggtggcgaa cttgatgcca aactgccgtt ccaccgcgat gatcaggttg
     1201 atgtgcgtga gcgagtccca cccatcgaca tcgtctgcgg tcatcgcatc gctcaacacg
     1261 atctcatcat cgttgaagac gttgcgaaag accgtctgcg tatgctcgcg gatcgattcg
     1321 ggcgagatgc tactcatgtg cggtgcgctc ctggataaag gtgggccatt cggccgcggc
     1381 ggacagtgga agctgccacc agctttgctg gccgacctcg ccggagatct tggcgaagcc
     1441 aaggttggcg tagtgttcga tgaccagcgc gttcttgggc gtcgggatgt attcgccgcg
     1501 aagggcggtc aggccgcgct gtcgcgccat gcggcacagg acgttgcgga cgaacgattc
     1561 aacgccgcgc ttcagcaccc ggcagctcat cagccaggtg tcgatcacca gctcgtcagc
     1621 ttcgactcgg ccgatgacga cgctgatcaa cccgttgtcc ccaaagcgat cggccaatga
     1681 gacggtgagc gtgagataat cggggctgcc ggccatcttc atcagcgccg ccgccgagtg
     1741 ccggcgggtg gtcaggttga actggttcga gcgcgcaacc agctgcgcga cgcgctcgag
     1801 gttggcggag ttgatcgggt cgatgcgcgc ccgcatgtcg agcgagcgca ggaaatcttc
     1861 gacgccgccg gacgacactt ccgcctgctg ccgcttggcg ttggcgcgat acatctcggt
     1921 gcgctgcagg tcttcagccc cgacggaaac ggtttgaaaa taggcgtggc gatcgatcgc
     1981 ctggatgtat cccgcggggt cttcgggcaa ttccgggacg gccacctcgg gcgcgagttg
     2041 ccgtacgagt gcgcgctcga cggggttgtc atcgacgaag acgagcgagt cgaggccgat
     2101 ctccagcgtg cgggcgatca gccggaggtt gcgggctttg tcgtcccagt tggcgacgaa
     2161 ggcggcgatg tcctcgatgc gcaggaccat ctccgggtgc ttctcgaacg cctcgcgcgc
     2221 gacggcctcg gtgttcttgc tgcagaccgc caggatcact ccgcggcggg ccaggtcgtt
     2281 gcaataatgc tggaaagcct ggaacgcttc gccctcggcg tcgccttggc cgagtcgaat
     2341 gccgccgatg ccgtcgtcgc cgatcacgcc gccccagagg gtgttgtcga gatcgagcac
     2401 gaggcatttt ttcgaagcgc cggcttgagc cgcgatgatc gacgcgacgc tgtgcgcgta
     2461 accggcgagg aactccggcg cgcacggcag cttcgcgtga tagaaaaagc ggtcgtcgcc
     2521 ccaagcccat cgaccggccg acgcggcgag gtgatcgaca tcgtgaatgg tgacgtaaga
     2581 cggggcggcg tcctgcatcg cgtggttcag cttcgagatg aaccgggcga gcccggcggg
     2641 gtggcgcatc tcgtggttgt gcatcacgcg ccacggcgg
//
LOCUS       DATIMH010000696         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3917218, whole genome shotgun sequence.
ACCESSION   DATIMH010000696 DATIMH010000000
VERSION     DATIMH010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3917218"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1247
                     /locus_tag="VLI90_12220"
     CDS             192..1247
                     /locus_tag="VLI90_12220"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HSV15016.1"
                     /translation="MFPLVAAAATTAATHPALSQPLHNNYLLSVLLGIIEGLTEFLPV
                     SSTAHLRILEKLATDYHWADIDLSTGYWKMYTVVIQLGAILCLPIYFLPRILKFIRTF
                     PRGERGDRNIFNHPLSLTMIAFVVTAGPAYLLAKKIGENLDNFYVIGSALLIGGIVMW
                     IIDAIFGERPSKFEGFDAVTAAIVDHGRLAPQPADGQPPRLQSVKTRHMEQMTVFQAI
                     WIGACQILSAVFPGTSRSMATIAAGQLAGLNRPTALEFSFFVSIPTMGAATLFALKKA
                     LKPGVGQAADVFSPKTMTHEQWVTLGIGFAVSFIVAMGVVAWFMSWVRRRGFAPFAIY
                     RIILGAAVLWYAWRGVS"
     gene            complement(1497..2612)
                     /locus_tag="VLI90_12225"
     CDS             complement(1497..2612)
                     /locus_tag="VLI90_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15017.1"
                     /translation="MNASLRRFDVSIVERLESRRLLSVAISTAATAGAAAGGDVSAAP
                     STVVLTGTALHAQTNQFFRAVVATIRNLPLLSSVYRFRASINWGDGKASSFANFVRQA
                     DGSIAVIGAHTYASVGVDDITVMITAVPPAWSALPVRIVGTFKSSATVFAPDGGVTIE
                     ATAGVHVTATVGFFRTTLSSTLLGATIDWGDGQQSAGKILALPTADPLAGGAFAVVGE
                     HTYSAVASDLVHVTVYELTPVGAAPPTTIIAEIDSVVDVLPISPVAALLPGDANGDGV
                     VDVADLGAIASNYGDAGGATWDMGDFNGDGAVDVGDLGALASSYGNVASYADPAAATS
                     AATDSSSTSSASAVVIHSPTATVASLIDGLFSVVAIA"
     gene            3025..>3553
                     /locus_tag="VLI90_12230"
     CDS             3025..>3553
                     /locus_tag="VLI90_12230"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSV15018.1"
                     /translation="MSNVKRSWLCGGVVLFAAALAITQFFPDKSRNVFAAPPNSPVGG
                     GSNSSSKQPLDSKDVSLDQAQSIIDAAMKKAQQLNIKEDIAVVDAGGNLKAFVRMDYA
                     WTGSIDIAIKKAKTSRMFDMPSGEIGKQAQPGGPLYHIEFSNGGLITFPGGVPLRTAS
                     DTVIGAIGASGSTVEN"
BASE COUNT          657 a         1131 c         1128 g          637 t
ORIGIN      
        1 agtggcgctt cgatatttga cgaaccccat ttagcccggc gtgaacacac gccgggtcaa
       61 cgtgggactt gaaggacctc cgattgagaa atcaaaacgc gcgcaacgtt cgaagcgaaa
      121 cccggcgtgt gttcacgccg ggctaaatgg ggttatccta cgaccgtcga ttctcaggga
      181 aggagaacaa cttgtttccg ctcgtcgccg ccgccgctac caccgcggca acgcatccgg
      241 cgttgtcgca gccgctgcat aacaactacc tgctgtcggt cctgctcggc atcatcgaag
      301 gtttgaccga attcctgccc gtcagctcca ccgcgcacct gcgcatcctg gaaaagctgg
      361 ccactgacta tcactgggcc gacatcgacc tgagcaccgg ctattggaag atgtacacgg
      421 tggtcatcca actcggcgcg atcctctgcc tgccgattta cttcctgccg cgcattctca
      481 aattcatccg caccttcccc cgcggtgaac gaggcgaccg caacatcttc aaccatcccc
      541 tgagcctgac gatgatcgcc ttcgtcgtca ccgccggacc ggcgtatctg ctggcgaaaa
      601 agatcggcga gaatctcgac aacttttacg tcatcggcag cgcgctgctg atcggcggga
      661 tcgtgatgtg gatcattgac gccatcttcg gtgagcggcc gtcgaagttc gaaggcttcg
      721 acgccgtgac ggcggcgatc gtcgatcacg gccggctcgc cccgcagccc gccgatggcc
      781 agccgccccg gctgcagagc gtgaagacgc gccacatgga acagatgacg gtgtttcagg
      841 ccatctggat cggcgcctgc caaatcctgt cggccgtctt ccccggcacg agccgatcga
      901 tggcgacgat cgccgccggg cagttggcgg ggctcaatcg gccgaccgcg ctggagttct
      961 cgttcttcgt ctcgatcccg acgatgggtg cggccacgct gttcgcgctg aagaaagccc
     1021 tcaagcccgg cgttgggcaa gccgctgatg tcttttcacc gaagacgatg acgcacgaac
     1081 aatgggtgac gctgggcatc ggcttcgccg tctcgttcat cgtcgccatg ggcgtcgtcg
     1141 catggttcat gtcctgggtg cgccgacgcg gcttcgcccc gttcgcgatc tatcgcatca
     1201 tcctcggggc cgcggtgctg tggtacgcgt ggcgcggcgt gtcgtgacgc gtttgattca
     1261 ctggcacgta attgggaaag agcgcacgcc tgcgcgctgt cattctgagc gccggcgtac
     1321 tcgccggagt cgaagtatct ggctggcgcg tcggcgaacg atgactcgcg cgaatcgcgc
     1381 aggagccgct tgcggcttcg cagacggaca aaccgcggtc actgatctac caaaccgcca
     1441 gcgactgctg cgccgaatgc gcgatctagc atccatcctt taaatgaccc aacagctcaa
     1501 gcgatcgcca ccaccgagaa caggccatcg atcagcgagg cgacggtcgc cgtcggactg
     1561 tggatgacaa cggcgctggc gctgctggtg ctggaagaat ccgtcgccgc gctagttgcc
     1621 gcggcggggt cggcgtagga agcgacgttg ccgtagctgc tggcaagcgc gcccaaatcg
     1681 ccaacatcga ccgcgccgtc gccgttgaag tcgcccatgt cccacgttgc cccgccggcg
     1741 tcaccgtagt tgcttgcgat ggcgcccagg tcagcgacgt ccacgacgcc gtcaccattg
     1801 gcgtcgcccg gcagcaacgc ggcgaccggc gaaatcggca ggacatccac cacgctgtcg
     1861 atctcagcga tgatcgtcgt gggcggcgcg gcgcccactg gcgtcagttc gtagaccgtc
     1921 acgtgcacca gatcactggc gaccgcgctg tacgtgtgtt cccccaccac ggcgaacgcg
     1981 ccaccggcca gcgggtcggc cgtcggcagc gccaggattt tgcccgcgga ctgctggcca
     2041 tcgccccagt cgatggtcgc gccaagcagc gtggacgaga gcgtcgtgcg gaagaagcca
     2101 accgtggcgg tgacgtgcac cccggcggtt gcttcgatcg tcaccccgcc gtcgggcgcg
     2161 aatacggtgg cggaactctt aaacgtgccg acgatccgca ccggcaacgc cgaccacgcc
     2221 ggcgggacgg cggtgatcat gaccgtgatg tcatcgacgc cgaccgacgc gtaggtatga
     2281 gcgccgatca ccgcgatcga accatcggct tgtctgacaa agttggcgaa ggagctggct
     2341 ttgccatcgc cccagttgat cgacgcgcgg aagcggtaga cgctggacag tagcgggagg
     2401 ttgcgaatcg tggcgacgac ggcgcggaag aactggttgg tctgcgcgtg cagcgccgtg
     2461 ccggtcagca cgacggtgga cggcgcagcg ctgacatcgc cccccgcggc ggcgccagcc
     2521 gtggcggcgg tgctgatagc aaccgacagc aaccggcgtg actcgagacg ttcaacgatc
     2581 gacacatcaa acctgcgaag agacgcgttc atcgggtcct cctcgaaggc ggcaggcgtg
     2641 cgaataccgt cctctgaaat gttgcggcag agaaacatat tactgaatgg ccggtcggaa
     2701 tagcaacatt ttcgtcggct ggccgactcc tcggcgcatc gaagcccccc acgccgggtt
     2761 cgaagcgcct gccctgagcc tgtcgatggg tactcgcgac gacccggatc ctgcggcgcg
     2821 cgagaaaaat ccgggtcgtc ggagtacctc cgaacccggc gtgggtggtt ccttcatgtc
     2881 ccacggcaga gccgtgggct ttagggcggg gctaatgact tcaggtagcg tccgattgtc
     2941 cgttcacctc cgctcggttg ctttttagga tcgcacccat cgtgtcgtgc ctatcgagtc
     3001 ctccagcaag cgaaggatga agctatgagc aacgtcaaac gatcctggct gtgcggcggc
     3061 gtggtcctgt tcgcggcggc gttggcgatc acgcagttct ttcccgacaa atcacgcaac
     3121 gtcttcgcag cgccgcccaa ttcgccggtg ggcggtggga gcaattccag ctcgaagcag
     3181 ccgctcgata gcaaggatgt ctcgctcgat caggcgcagt cgatcatcga tgcggcgatg
     3241 aagaaggcgc agcagctgaa catcaaggaa gacatcgccg tcgtcgatgc cggcgggaac
     3301 ctgaaggcgt tcgtgcggat ggattacgcc tggaccggca gcatcgacat tgcgatcaag
     3361 aaggcgaaga cgtcgcgcat gttcgatatg cccagcggtg agatcggcaa gcaggcgcag
     3421 cccggtgggc cgctgtatca catcgagttt tccaatggcg ggttgatcac gttccccggc
     3481 ggtgtgccgc tgcggacggc gagtgacacc gtcatcggcg ccattggcgc gtcgggatcg
     3541 acggtggaaa acg
//
LOCUS       DATIMH010000697         6012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2186834, whole genome shotgun sequence.
ACCESSION   DATIMH010000697 DATIMH010000000
VERSION     DATIMH010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186834"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1776)
                     /locus_tag="VLI90_12235"
     CDS             complement(<1..1776)
                     /locus_tag="VLI90_12235"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HSV15019.1"
                     /translation="MRHPKLQHACNAVLAERLERRQLLAGTGLDVTPTLNANSIVHAL
                     LGGNTGIVVTSFHLSAQQKGAVLSSGTFTNPGGVFGLGNGIIMSTGDVSQYTSPPPTS
                     PQTSTQYGGTTANSGKATDAQEALLVPISGNQISHFDVTELDLTFNMPKTLGQIFFNV
                     VFGSAEFPQFVGSEFKDAFGLYVNGTNIATVDRQPVNIDHPAFVADTHTALNGLLAPG
                     NDPLLHFSTFVGAGTRNNHLTFIVGDTSDHALDTTVYISALGALPPNPNIPVATVSAP
                     DVTASQPTATFTVTYTDPNVPIQVGSIDSNDLIVTGPNGYSQIATLISVDNPTDGSPR
                     VATYSVTGPSGGPFTVADDGTYVVSMQFQQVQDTAGDFVTEGGLGSFAVAVPGVTLAA
                     DPTFNAGTPKYNFSVTYIDPTGVKLTTIDNSDILVTNPANSFSQLAKRVSVSSDGDGR
                     FTANYSLAAPNGKTWAVIDAGSYTFALQNGAVLDLNGSALPAGTLGTVVLTPFGGLSA
                     PAAGEIPASGGAVAVAGDALGRQFIAYFDTVDSDLKYFTRMPNGAVSPNRVIDASPGA
                     GAQLGIAIQHNLPVVAYYDSTAQQLK"
     gene            complement(2079..3317)
                     /locus_tag="VLI90_12240"
     CDS             complement(2079..3317)
                     /locus_tag="VLI90_12240"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HSV15020.1"
                     /translation="MPQATPRTHFAAIIALMLASLLTTAPTVHAAAKRQQIGQASGSL
                     GSATIDGQRLGIGAPVFEGSRIVTGDNDSASVLLNSKVVLKFDANTAAVVSEAKGTHV
                     QLEQGNVEVFVARRLPGAGAVALSDPDATIEALGTVFIAGYSPADRGGWYATVESTRP
                     RGVSVRGNADPGATDLPPGQFVRLQAGKLMNPPAGTDPGELKQHLGTIGNLDKALGKH
                     AGAVSRQKAAVTEFVRVQRSINGTAFNQTLQNALASHTGNDARTDAIQYQSVTTLVSH
                     GVITSSGGSTSGGGGGAGGVSSGGFSDISVTLGSGGVLTFRLFDAGTPDGDQVALRVI
                     SNGSTVLNVPSITLTNTGTQFSPKVSAGNVAISLTALNDGSIPPNTGGVTLQNVTAGH
                     AQQTYSVNTGQVATLQVKTH"
     gene            complement(3544..5187)
                     /locus_tag="VLI90_12245"
     CDS             complement(3544..5187)
                     /locus_tag="VLI90_12245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV15021.1"
                     /translation="MPRLRAAAWCVCGALSVLPLSGKSVFAQDEAPATTAAAATDVTT
                     SQPSAREATVRTGEAEVLFNQARISYYNGSFGDAETAFRRCISLNPNDAQAYYYLGLS
                     LTGQDRAAAAIGAFNQAVGLDPSLSEARAGRASAEIRDHQYNAAQSDIDQLATDAQYA
                     DTAAYLRGQLAYARGDYQSAASSFNQARAAGGEEASAAGLYQGLSYIQLKQLNRAQAA
                     LRDVARIDRDSSVGAASRAMSDSIDVNTGRSRPFTLQVAAGIEYDSNVPLVQNNSLLP
                     PGTKHQQDARFVLQPRASFSFVRNDRLELGIDTTDYFAWQSYDNDFDIASYGVGAFGN
                     FRINPKLFAGLRYDFNYLELGHDPFLKRNTVTPNLTFIEPKIGYTTAYYQFEARQFDP
                     VPPTKVLDRDSHNNVLGIVQGIDFPTFARNGLQAPRVEVGFRYENQDATGSDFDGNFF
                     TLGGAYYMPICKKWTADAGLDATYFAYDHANSLDKNGRERSDYEVRPHLGVTWQANRY
                     VALRGDYTFTYHSSNVEAGNVRPYAYNQHVVGVRAIVTY"
     gene            5525..>6012
                     /locus_tag="VLI90_12250"
     CDS             5525..>6012
                     /locus_tag="VLI90_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15022.1"
                     /translation="MTQTIDQRETRPRRLRRLGVVALVVMLLAIGFAVAWNNGLRDQV
                     VPRNFGIVEAGRLYRSGQISARLIEPTLREHHIQVIVALAAAGMKLQDLAAEEHAAAA
                     LGIDRELFPLSGDGTGSAAVYAAAVAAIDKALFPAPRDPAERYELQMLKGECRLQLRD
                     GLG"
BASE COUNT         1025 a         1851 c         1990 g         1146 t
ORIGIN      
        1 cttcagctgc tgtgcggtcg aatcgtaata cgcgacgact ggcaggttgt gctggatcgc
       61 gatgcccagc tgcgcgcccg cgccggggct ggcgtcgatc acgcggttgg gcgagaccgc
      121 gccattcggc atcctcgtaa aatattttag atcgctatcg accgtatcga aatatgcgat
      181 gaactgccgg cccagcgcat cgccggcgac ggcgaccgcg ccgccggatg cgggaatttc
      241 gcccgcggct ggcgcgctca atccgccgaa tggcgtgagc acgacggtcc ccagcgtgcc
      301 ggccggcaac gcgctgccgt tcaggtcgag caccgcgccg ttctgcaggg cgaacgtgta
      361 cgacccggcg tcgatgacgg cccatgtctt gccgttgggc gcggcaagcg agtagttcgc
      421 ggtgaatcgg ccatcgccat cgctggagac cgacacgcgc ttggccagtt gcgaaaacga
      481 attggcgggg ttggtcacga ggatatcgct gttgtcgatc gtcgtcagct tcacgccggt
      541 cggatcgatg taggtcacgc tgaagttgta cttgggcgtg ccggcattga aggtgggatc
      601 ggccgccagc gtcacgcccg gcacggccac ggcgaagctg cccaggccgc cttcggtgac
      661 gaagtcgccg gcggtgtcct gcacctgctg gaactgcatc gacacgacgt aggtgccatc
      721 gtccgccacc gtgaacggcc cgccgctggg accggtgacg gagtacgtcg cgacgcgcgg
      781 cgagccgtcg gtggggttat cgacgctgat cagcgtcgcg atctgcgaat agccgttggg
      841 ccctgtcacg atcaggtcat tactgtcgat cgatccaacc tggatcggca cattcggatc
      901 ggtgtaggtg acggtgaacg tggcggtcgg ctgcgacgcc gtcacgtccg gcgcgctcac
      961 ggtcgccacc gggatgttcg ggttcggcgg cagcgcgccc agcgcggaga tgtacacggt
     1021 cgtatccagg gcatggtcgg acgtgtcgcc gacgatgaag gtcaagtgat tgttgcgcgt
     1081 gccggcgccg acgaaggtgg agaagtgcag cagcgggtcg ttgccgggcg ccagcaaacc
     1141 attcaacgct gtgtgcgtgt cagccacgaa cgcggggtga tcgatgttga cgggctggcg
     1201 atcgacggtg gcgatgttgg tgccgttgac gtacaggccg aaagcgtcct tgaattccga
     1261 cccgacaaat tgcgggaact cggccgagcc gaatacgaca ttgaagaaga tctgccccag
     1321 cgtcttgggc atgttgaagg tcaggtccag ctccgtcacg tcaaagtgcg aaatctggtt
     1381 gccgctgatc gggaccagca aggcttcctg ggcatcggtg gccttgccgg aattggcggt
     1441 ggtgccgcca tactgcgtgc tggtctgcgg cgacgtcggc ggcggtgagg tgtattggct
     1501 gacatcaccc gtgctcatga tgatgccgtt gcccagcccg aaaacgccgc cgggattggt
     1561 gaacgtgccg ctggagagca ccgcgccctt ctgctgggcc gacaggtgga acgacgtgac
     1621 gacgatgccg gtgttcccac cgagcagggc atggacgatc gagttcgcgt tcagcgtggg
     1681 cgtgacgtcc aagccggtgc cggccaggag ctgccgccgc tcgagccgct cggcgagaac
     1741 ggcgttgcag gcgtgttgaa gtttcggatg tcgcataatc attcctgccc tgactaactg
     1801 gtacgcgctt ttccccttct cccggtacac cgggagaggg acggggtgag ggtgatgcgt
     1861 ttaatgacca atgggcaaat gccaaatccc aatgaagtcc caagcaccaa tggcccattg
     1921 ggacttggtc cttggaagtt cattgggatt tggcgcttgg gcattggtca tcgtcgttcc
     1981 aaatcaccct caccctatcc ctctcccgga ttaccgggcg aggggaccag agcgcgggtc
     2041 tctcacgtat tcacgtaatc acgtcttcac gccctcactc aatgcgtctt cacctgcagc
     2101 gtcgccactt ggcctgtgtt caccgagtac gtctgctgcg cgtgacccgc ggtcacgttt
     2161 tgcagggtta cgccgccggt gtttgggggg atgctgccgt cgttcagggc cgtcagagaa
     2221 atcgcgacgt tgccggccga taccttcggg ctgaactggg tgccggtgtt cgtcagcgtg
     2281 attgacggca catttaagac ggtgctgccg ttgctgatga cgcggagcgc gacttgatcg
     2341 ccatcgggcg tgccggcgtc gaagaggcgg aaggtgagca ccccaccgga accgagggtc
     2401 acgctgatgt cggagaaccc accgctcgaa acgccgccgg cgccaccgcc accgccgctt
     2461 gtgctgccgc cgctgctggt gatgacgccg tgcgagacga gcgtcgtcac cgactggtat
     2521 tggatggcgt ccgtcctggc atcgttgccc gtatggctcg ccagtgcatt ttgaagcgtc
     2581 tggttgaacg cggtgccgtt gatggatcgc tgcacgcgga cgaattccgt cacggccgcc
     2641 ttctggcgcg agacggcgcc agcgtgcttg ccgagggctt tgtcgagatt gccgatcgtg
     2701 cccaggtgct gcttcagttc acccgggtcg gtgcccgcgg gtgggttcat cagcttgccc
     2761 gcctgcagtc ggacgaactg ccccggcggc agatcggtcg cgcccggatc ggcgttgccg
     2821 cgcaccgaca cgccccgcgg gcgcgtggac tcgaccgtcg cgtaccaccc gccacgatcg
     2881 gcgggcgaat agccggcgat gaagacggtg ccgagcgctt cgatcgtcgc atccggatcg
     2941 ctcagcgcca ccgcgccggc cccgggcaat cgcctggcga cgaacacctc gacgttgcct
     3001 tgttccaatt ggacgtgcgt gcccttggcc tcgctcacca ccgcggcggt gttggcgtca
     3061 aacttcagca ccaccttgct gttgagcagg acggaggccg agtcattgtc gccggtgacg
     3121 atccggctgc cttcgaacac gggcgcgccg atgccgaggc gctggccatc gatcgtcgcg
     3181 gaaccgagcg atccgctggc ttgaccgatt tgttggcgct ttgccgcggc gtggactgtt
     3241 ggggccgtcg tcagcaagct cgccagcatc agggcgatga tggcggcgaa atgcgttcga
     3301 ggtgtggctt gaggcataag cttctctgtt ttcaaaacga tgaagaggaa tagccgcgac
     3361 aagacgcaaa aaacgcagaa aagacatcgg cctttccttt tgtgcctttt gcgttttttc
     3421 gtggcaaatc tcttccttgc tcatatcgat cgatcgttcg ctcacttgac gtccgcgaaa
     3481 tctcggccct tcgggccgcc gctaaaagga ggttattctg gactggcggc tcctctttct
     3541 ttcttaataa gtcacgatcg cccgcacgcc gaccacgtgc tggttgtacg cgtatgggcg
     3601 gacgttgccg gcttcgacgt tggacgagtg ataggtgaac gtgtaatcgc cgcggagcgc
     3661 gacgtagcgg ttcgcctgcc aggtcacacc caggtgcggg cggacttcgt aatcgctccg
     3721 ctcgcggccg tttttgtcca ggctgttggc gtggtcgtac gcgaagtacg tcgcgtccag
     3781 ccccgcgtcg gccgtccact tcttgcagat cggcatgtag tacgccccgc cgagcgtgaa
     3841 gaaattcccg tcgaaatcgc tgccggtggc gtcctggttc tcgtagcgaa accccacttc
     3901 cacccgcggg gcctgcaggc cgttgcgggc gaaggtcggg aagtcgatcc cctgcacgat
     3961 gcccagcacg ttgttgtgac tgtcgcgatc gagcaccttg gtcggcggga ccggatcgaa
     4021 ctgcctcgct tcgaactggt agtacgcggt ggtgtagccg atcttcggct cgatgaacgt
     4081 gaggttcggc gtcaccgtgt tgcgcttcag gaacgggtcg tgacccagct ccagataatt
     4141 gaagtcgtac cgaaggccag cgaagagctt tgggttgatg cggaagttgc cgaaggcgcc
     4201 gacgccgtag gacgcgatgt cgaagtcatt gtcgtacgac tgccaggcga agtagtcggt
     4261 ggtgtcgatg cccagctcca gccgatcgtt tcgcacaaaa ctgaagctgg cccgcggctg
     4321 caagacgaaa cgggcatcct gctgatgctt ggtgcccggc ggcagcagcg agttgttctg
     4381 cacgagcggg acgttgctgt cgtactcgat gcccgcggcc acctgcaggg tgaagggccg
     4441 cgatcggccg gtgttcacat caatggaatc agacatcgcg cgcgatgcag cgccgaccga
     4501 tgaatcgcga tcgatccgcg ccacatcgcg caacgcggcc tgcgcgcggt tcaactgctt
     4561 cagctgaatg taagacaatc cctggtacaa ccccgcggcc gatgcttcct cgccaccggc
     4621 ggcgcgggcc tggttgaacg agctggcggc cgactggtag tcgccgcgcg cgtatgccaa
     4681 ttgcccgcgc agatacgcgg cggtgtcggc gtattgggcg tcggtagcta gctggtcgat
     4741 gtcgctctgc gcggcgttgt attgatgatc gcggatctct gccgatgccc ggccggcgcg
     4801 cgcttcggac aacgatggat ccagaccgac tgcttgattg aacgcgccaa tcgcagcggc
     4861 cgcacgatcc tggccggtca aggacaagcc gaggtagtaa tacgcctggg cgtcgttcgg
     4921 attgagcgag atgcaccggc ggaacgcggt ttcggcgtcg ccgaagctgc cgttgtaata
     4981 gctgatccgc gcctggttga agagcacttc cgcttcaccg gtgcgaacgg tggcttccct
     5041 cgcgctcggt tgcgaggttg ttacgtccgt cgccgctgcc gcggtcgtcg ccggcgcttc
     5101 atcctgcgcg aaaacggatt tgcccgacaa cggcaacacg gacagcgcac cgcagacaca
     5161 ccacgcggcg gcccggagcc ttggcattgc agcgcccata caccttccct ttaacaccca
     5221 cgaccgagag acatgaatcg gcccgccatc ttcggcggtg aaccgcttct attcaagaaa
     5281 aatagcagag ttgtgggcgg acgatgcaaa ggtgacaaag atatcaacag cctgggcacg
     5341 aattatggcg gcacgtccat gtaatcgcag ccgcaggcga cagccagcgg gggcgcgaga
     5401 acgctaaccc tttgatatcc gaaagatccc gctggctgtc gcccgcggct actacggatg
     5461 cgcgcgcccg ctcacgtttt tgacgccgtg cgaaccccgc gatagcgtgg aacgttgcat
     5521 ccaaatgacg cagacgatcg atcaacgcga gacgcgcccg cgccggcttc ggcgcctcgg
     5581 cgtggttgcg ctggtggtga tgttgctggc gatcggcttc gccgtcgcgt ggaacaacgg
     5641 ccttcgcgac caggtcgtcc cgcggaactt cggcatcgtc gaagccggcc ggctgtatcg
     5701 cagcgggcag atctccgccc ggctgatcga accgacgctg cgcgagcatc acatccaggt
     5761 gatcgtcgcg ctggccgcgg cggggatgaa actgcaggac ctcgccgccg aggaacacgc
     5821 cgcggcagcg ctgggcatcg accgcgagct gttccccctg tccggcgacg gcaccggcag
     5881 cgccgcggtc tacgccgccg ccgtcgcggc gatcgacaag gcgctcttcc ccgcaccgcg
     5941 agacccggcc gagcggtacg aattgcaaat gctcaagggc gagtgtcggc tccagctgcg
     6001 cgacggcctg gg
//
LOCUS       DATIMH010000698         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1903497, whole genome shotgun sequence.
ACCESSION   DATIMH010000698 DATIMH010000000
VERSION     DATIMH010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903497"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1435
                     /locus_tag="VLI90_12255"
     CDS             152..1435
                     /locus_tag="VLI90_12255"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSV15023.1"
                     /translation="MTDQADAEARERVGAVYRGESRRVFATLVRLLGDFDLAEEALHD
                     AFASAMEQWPRDGIPANPRAWLVSAGRFKGIDAIRRRARSDATMAEFAERLNARDDGG
                     ADRPEPDEAGIEDDRLRLIFTCCHPALPAEARVPLTLREVCGLTTEEIARAFLTTAPT
                     LAQRIVRAKAKIRTAKIPYQIPSRADLPERLDTVLQVIYLVFNEGYSASSGESLTRAE
                     LSSEAIRLGRLLMELLPEPEVLGILALMLLQESRRAARTSSSGELVLLEDQDRSLWNR
                     EQIAEGLSLVRRALATRRVGPYSLQAAIAAVHAEAPSATATDWAQIAGIYDVLLQIEP
                     SPVIELNRAVAIAMRDGPAAGLAAIDQIFSRGDLADYHLAHAAKAEFCRRLGRTDDAR
                     AAYARALELAGQETERRFLQQRLSELGAPPNPDQR"
BASE COUNT          256 a          477 c          534 g          275 t
ORIGIN      
        1 ccgtgacacg ggcctccggc ccgtgcttgc agctctcgaa cgtcagcagt tcatcacccc
       61 ccatcttctg gttcatctgt gtctatcggt gtgcatctgt ggttaaaacc tcttcgtctt
      121 ttcccgaacg tccgtgctga aaccgacttc tatgaccgat caagcagatg ctgaggcgcg
      181 ggagcgagtt ggcgcggttt atcgcggtga gtcccgccgc gtcttcgcca cactcgttcg
      241 attgctcgga gatttcgatc tcgcggaaga ggcgttgcac gatgcgttcg cgtcggcgat
      301 ggagcagtgg ccgcgcgatg ggatccccgc gaacccgcgg gcgtggctgg tgtccgcagg
      361 gcgattcaag gggatcgacg cgattcgacg ccgggcgcgt tcggatgcga cgatggcgga
      421 atttgccgag cggttgaacg cgcgcgacga cggcggcgcc gatcgtccgg agcccgacga
      481 agcggggatc gaggacgacc ggctgcggtt gatcttcacg tgctgtcacc cggcgttgcc
      541 ggccgaagcg cgggtgccgc tgacgctgcg cgaagtatgc ggactgacga cagaggaaat
      601 cgctcgcgcg tttctcacca ccgcgccgac gctggctcag cgcatcgtcc gcgccaaggc
      661 gaagattcgc accgcgaaaa ttccgtatca aatcccatcg cgtgcggatt tgccggagcg
      721 gctcgacacc gtgctgcagg tgatttacct ggtgttcaac gaaggatatt cggcgtcgtc
      781 cggcgaatcg ctcacgcgcg cggaattgtc gagcgaggcg attcgcctcg gccggctgct
      841 gatggaattg ctgcccgagc cggaagtgct cggcatcctc gcgctgatgc tgctgcagga
      901 atcgcgcagg gcggcgcgca cgtcgtcatc cggcgaactg gtgctgctgg aagatcagga
      961 ccgatcgctg tggaaccgcg agcagattgc cgagggcttg tcgctggtgc ggcgagcgct
     1021 ggcgacacgg cgcgtcgggc cgtattcact tcaagcggcg atcgccgccg tgcacgccga
     1081 ggcgcccagc gccaccgcga cggactgggc gcagatcgcg gggatctacg atgtgctgct
     1141 gcagatcgaa ccgtcacccg tgatcgagct gaatcgagcc gtggcgatcg cgatgcgcga
     1201 cggccccgcc gcggggttgg cggcgatcga ccagattttc tcgcgcgggg atttggcgga
     1261 ttatcacttg gcccacgcgg ccaaggcaga gttctgccgg cggctggggc gcaccgacga
     1321 tgcgcgggcg gcatacgcgc gcgctctgga actggctgga caggaaacgg agagacggtt
     1381 cttacagcag cggctgtcgg agctgggggc gccaccgaat cctgaccagc gctagcgcca
     1441 aaagaagccc cgcatgtaca tgcggggctt cgggcagggc agacacatgt aggaaccgcg
     1501 cgctctggtc ccctctcccg gtactgctga ccgcaggtcg cc
//
LOCUS       DATIMH010000699         7551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_746940, whole genome shotgun sequence.
ACCESSION   DATIMH010000699 DATIMH010000000
VERSION     DATIMH010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746940"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..2966)
                     /locus_tag="VLI90_12260"
     CDS             complement(48..2966)
                     /locus_tag="VLI90_12260"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HSV15024.1"
                     /translation="MTPSTQHTPDAELIDAYVTGGLAADERAAFEAHLRNCPTCQSTV
                     ASARSSDEAMRNLFDNARPTAGFEDRMIRAVREERSPRLRIPLPVRRAAAVAAAVLLV
                     GGVGYIGNGLIENGNGLDRVLAASNLRQLGQAILLYTNENKQEYSRTHELSARQPVSI
                     HPQDGQNVLYGDGHVKTQNNPFQGTQRDKIFTGRTSQGSPKRLESTDNILLPTDDEPA
                     KEKQGQSYQEHPSAGNDAKALPPPAAFKPEEFVLGRMPAMLDGKNAYGANIVVSSGTL
                     SLATTASGKPIDAPQAAIAGVANGDAGNHPSAPQTAPPPTTTTAVDSRRIIRNGTMEF
                     EVDSFDAAVARITQIVGESGGFVGTVDSQKLPNGKVKGTVTLRVPPERLDALVLSLRA
                     LGDLKSQQISAQDITKQYTDIQSQLTAARAMESRLLELIKSGNGAIKDLLAAEKELGV
                     WREKIEQAEGEIRYYNNLVSLSTLSITIYERDIKTPAAATETETADVGIEAEDVEGAR
                     TDAIKAIEDANGRTIESELKRFDAGQLAARIVADVPPESAGPVIDRLKQLGRAARLEV
                     HRQQTPADGSEPTTAGTTLRVDRKPTRLLISLYNLANVAPRRTTNITLASADVEATYA
                     ALLAQAKGADGRIVTSSVDRGDPAKASGSLVLELSPEKFDSALSAVRGQGDVLKLAAV
                     ENPDTQNTTLAKQGLTIQLVSLASVQPRETVQQTIATAAVPAAYHAILDAASAAHGRV
                     TNAQLNENDRQNITGVIDVEVPRSELASMENTIATAGDSVARAASRTADAENTVDSKV
                     LLRMSIVAADRLPPRETTTMQVEVGDATKSTGDAQTIALASGGTVLDATTQRDRGGAQ
                     RAHVVLDVPLSKAGDVLNQLRGSGVVRAIESSRDANAPTGALARARLDVTYATADAIV
                     SGQSSLWSSIRNGLATSIAGLLLSVRLLVIGLCLVGPWLAMLWIAWRLWRRRRPTTTV
                     "
     gene            complement(2972..3616)
                     /locus_tag="VLI90_12265"
     CDS             complement(2972..3616)
                     /locus_tag="VLI90_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSV15025.1"
                     /translation="MAQPPLSETDETLVNRARRGDGPAFEELIRRAGKLVFARLYLET
                     GDAHEAEDLAQETFLIAFRRVGQVADATTFRPWLMSIAHSVVIDAARRRGRLKRRASA
                     DDVRSMSWASHHPSSPSDDAARNDECQRVLSILRSMPQEYRQPLTLRYLTGADYETIG
                     RELGLTNGSLRGLLHRGLAMLREQMRERRNPTESRPVPRPGISGRGRPGSPSNQ"
     gene            3721..3811
                     /locus_tag="VLI90_12270"
     tRNA            3721..3811
                     /locus_tag="VLI90_12270"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3757..3759,aa:Sec,seq:tca)
     gene            4078..6168
                     /locus_tag="VLI90_12275"
     CDS             4078..6168
                     /locus_tag="VLI90_12275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSV15026.1"
                     /translation="MEAIDGLTEQLASAATLIEADDLSALAELHGFFQQLDRWFEAHP
                     QGHPRHQQIRQASTQAQGVLEKLVLHEADDVDAAVNQVLALVSEIQRSASLAAESEQP
                     ATQAPNPVVTAAPIMPTPAPAAEALQAEKPVAVDDLPLVRDFIAESMSHMESAEAALL
                     KIEEAPDDIEAINSVFRSFHTIKGSAGFLNLKQIGALAHATENLLDLARKGKLSLVGD
                     VADVVLESLDAMKGLIMTLRDTAEAGQAAPSEPRVPELIQRLHAAARGEAVKTQPKAA
                     AAPAPAPQTTGAAAPATQPVKPAPQPAASKDAGADATVKVSTERLDSLINQVGELVIA
                     QSMVSQDMAATARANPKHARNLSHLGKITREMQDLAMSMRMVPIQGVFQKMARVVRDL
                     TKKAGKEVDFVTVGGETELDRNLVDSISDPLIHMVRNSCDHGIEMPDARVQSGKPRAG
                     RIELRAFHQGGSIAIQIIDDGKGLPKARILQKAIDAGIVKPGQELSEQEIFKLIFHAG
                     LSTAEKVTDVSGRGVGMDVVKRNVEALRGRIDITSTEGQGSTFTIRLPLTLAVIEGLV
                     VKVGSQRYIIPITSVEQTIQPKRDQLSTVQGRGEICLVRGAPLPLYRLHKIFNIEPRT
                     RDVTEALVLILHDNDRRCCLLVDELLGQQQVVIKSMGESLGTVRGISGAAILGDGSAS
                     LILDVAGIMDIAVA"
     gene            6257..>7551
                     /locus_tag="VLI90_12280"
     CDS             6257..>7551
                     /locus_tag="VLI90_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSV15027.1"
                     /translation="MSLTIGRRLTIGFGLCVAATAALGFFINKQLSAISQNVGHITSH
                     ALPGAAIAADIDAMVKTNVVLLLRHVTTSDAAEMSRLEGEFTKTKAHLDELFAEYQEL
                     SPVGDEGEAFGRLVQARKGWLSAKEMVITPSRAGQKKAAAEVFATRAVPLIDAINDAA
                     HGLLEANHTSGNAEGAQSVATIVTARRAIIGGITAAAVLALVVATLIIRSTNRSLRGM
                     AGSLVTGSEQVASASTQLSAASQSLAQGASEQAAALEETSAALEEMSSMTKKNAETAT
                     KASALSTEAHEAATRGNAAMQKMSAAVQDIQKSSGETAKIIKVIDEIAFQTNLLALNA
                     AVEAARAGEAGRGFAVVAEEVRSLAMRSAEAAKNTTALIEQSVQNTRNGVTIVDEVGK
                     NLQDINAACTGVKSLVGEIAAASTEQARGIEQVNTAVSQ"
BASE COUNT         1360 a         2351 c         2470 g         1370 t
ORIGIN      
        1 cgtcggcgtt ctcttggcct cttggcgcgt atacaaggcg ttcgcgttta aactgttgtc
       61 gtcggtcgtc gccggcgcca caggcgccag gcgatccaca gcatcgcgag ccacgggccg
      121 accaggcata ggccgatcac cagcaggcga acgctcagca gcaggcccgc gatgctggtg
      181 gctagcccgt tgcggatgct ggaccacagg ctgctctggc cgctgacgat cgcgtcggct
      241 gtcgcgtacg tgacgtcgag ccgggcgcgc gcgagcgcgc cggtgggggc gttggcgtcg
      301 cgcgaggatt cgatcgcgcg aacgacgcca ctgccgcgca gctggttcag cacatcgccc
      361 gcttttgaca gcggcacgtc gagcacgacg tgcgcccgct gcgccccgcc gcggtcgcgc
      421 tgcgtggtcg cgtcgagcac cgttccgccg ctggcgaggg cgatcgtttg cgcatcgccg
      481 gtggacttgg tggcgtcgcc tacctccacc tgcatcgtcg tcgtctcgcg cggtggtaat
      541 cgatcggcgg cgacgatcga catccgcagc agcaccttgc tgtccaccgt gttttccgcg
      601 tcggccgtgc gtgaggccgc gcgggcgacg ctgtcacccg cggtggcgat cgtgttttcc
      661 atcgatgcca actcgctgcg cggcacttcg acgtcaatca cgccggtaat attctgccga
      721 tcgttttcgt tcagctgggc gtttgtcacg cggccgtgag ccgcgctggc ggcgtccaga
      781 atggcgtgat acgccgccgg cacggcggcc gtggcaatcg tctgctgcac cgtttcgcgc
      841 ggttgcaccg acgccagcga caccagctgg atcgtcaaac cctgcttggc cagcgtcgtg
      901 ttctgcgtgt ccgggttttc caccgcggcg agcttcagca cgtcgccttg gccgcgcacg
      961 gccgatagcg ccgagtcgaa tttctctggc gagagctcca gcacgagcga accgctcgct
     1021 ttcgccggat caccgcgatc gacgctggat gtgacgatgc gtccatccgc ccccttcgct
     1081 tgcgccagca gcgccgcgta ggttgcctcg acatcagcgc tggccagcgt gatgttcgtc
     1141 gtccgtcgcg gcgcgacgtt tgcgagattg tagagcgaga tgagcagccg agtgggcttg
     1201 cgatcgacgc gcaacgttgt gccggccgtc gtcggctcac ttccgtcggc tggtgtttgc
     1261 tggcgatgca cttcgaggcg cgccgcccga cccagttgct tgagccgatc gatcaccggc
     1321 ccggccgatt cgggtggcac gtcagccact atgcgtgccg ccagctgccc cgcgtcgaat
     1381 cgcttcagtt ccgattcgat cgtgcgtccg ttggcgtctt cgatcgcctt gatcgcgtcg
     1441 gtgcgagcgc cctcgacgtc ctcggcttcg atgccaacgt ccgcggtttc cgtctccgtc
     1501 gcggcggcgg gggttttgat gtcgcgctcg tagatcgtga tcgacagcgt cgatagcgac
     1561 accaggttgt tgtaatagcg gatctcgccc tcggcctgct cgatcttctc gcgccagacg
     1621 cccagctcct tctccgcggc cagcaggtcc ttaatggcgc cgttgccgct cttgatcagc
     1681 tccagcagcc gcgattccat cgcccgggcg gcagtgagtt ggctctggat gtcggtgtac
     1741 tgcttggtga tgtcctgcgc cgagatctgc tggcttttca gatcgccgag cgcgcgcagg
     1801 ctcagcacca gcgcgtccaa ccgctccggc ggcacgcgca gggtgacggt gcctttgact
     1861 ttgccattcg gcagtttttg cgaatcgacg gtgccgacga acccgcccga ttcgccgacg
     1921 atctgcgtga ttcgcgcgac ggcggcgtcg aagctatcga cctcgaactc catcgtgccg
     1981 ttgcggatga tcctgcgact atcaacggca gtcgtcgtcg tcggcggcgg ggcggtctgt
     2041 ggcgctgacg ggtgattgcc ggcgtctcca ttggcaacgc cggcaatggc ggcttgcggc
     2101 gcgtcaatcg gttttcctga tgcagttgtg gcaagcgaga gggttccact gctcacgact
     2161 atgttggcgc cataggcgtt ctttccatcc aacatcgcag gcattcgtcc cagcacgaac
     2221 tcttctggct taaacgcagc aggcggcggc agcgccttcg cgtcattgcc agcagatgga
     2281 tgctcttggt acgactgacc ttgtttctcc ttcgcaggct catcgtcggt ggggagaagg
     2341 atattgtccg ttgactccaa acgcttcggc gacccttgcg acgtccgtcc cgtgaaaatc
     2401 ttgtcgcgct gggtgccctg gaatgggttg ttctgtgttt tgacatgtcc atcgccatag
     2461 agaacgttct gaccatcttg cgggtggatc gagactggct gccgggcact aagctcatgc
     2521 gtgcgcgaat attcttgctt gttctcatta gtgtaaagaa ggattgcctg cccgagctgg
     2581 cgcagattgg aagccgccag cacacgatca agcccgttcc cattctcgat caatccgttg
     2641 ccgatatatc caacgccgcc gaccagcagc accgcggctg cgacggcggc ggcgcggcgg
     2701 acgggcaggg ggatgcgcag ccgcggcgat cgctcttcgc gcaccgcgcg aatcatgcgg
     2761 tcttcaaatc ccgcggtggg gcgggcgttg tcgaataaat ttcgcattgc ctcatcgctc
     2821 gagcgggcgg acgcgacggt cgattggcaa gtgggacaat tgcgcaggtg cgcttcgaac
     2881 gcagcacgtt cgtcggcggc gagcccgccg gtgacgtacg cgtcgatcaa ttccgcgtcg
     2941 ggagtgtgtt gagtcgaagg cgtcatgttc gtcattggtt ggaaggagag ccgggccgtc
     3001 ctcggcccga aattcccggc ctggggacag gccggctctc cgtgggattt cgtcgttcgc
     3061 gcatctgctc gcgcagcatc gccaggccgc ggtgcagcag gccgcgcagg gatccattgg
     3121 tcaatcccaa ctctcgcccg atcgtttcgt aatccgcgcc ggtcagatac cgcagcgtca
     3181 gcggctggcg gtattcctgc ggcatggacc gcaggatcga caacacgcgc tggcactcat
     3241 cgtttcgtgc cgcatcgtca gacggcgatg acggatgatg cgaagcccac gacatcgaac
     3301 gcacatcatc cgcgctcgcc cggcgtttga gccggccccg gcggcgggct gcgtcgatca
     3361 ccacggaatg cgcgatcgac atcagccacg gccgaaacgt cgtcgcgtcc gccacctgac
     3421 caacccggcg gaacgcgatc aggaatgttt cctgcgccag gtcttccgct tcatgcgcgt
     3481 cgccggtttc aaggtacagc cgggcgaaca ccagtttccc cgcgcggcgg atcagctctt
     3541 caaacgccgg cccatcaccg cgtcgggcgc ggttaaccag cgtttcatcc gtctcactca
     3601 gcggcggctg tgccatgcgt cactccatta gacgacaggc ggccgcacgc ggcgcgcgca
     3661 tgcggcgaga attcttgaca gagtcaccac cggcgacgtc tgtgctatat ttggacgagc
     3721 cggagagtgc tgggttcctg gtggtccccg cggtcttcaa agccgtcgtc gccccgcaag
     3781 gggcgaggtg ggttcgattc ctaccctctc cgttcgactt gtttttgttg tcttcacccc
     3841 gcgcacaacg tgagcatttg gccaccggca tcgacgctgg ctggccccgg cgcgagcccc
     3901 ttcgtccaat aattttccga cagtacggca ccaaatcggg gatttccgac cgccgcacaa
     3961 gcatctggca ctgcaaatca tctaaagcgt acagcccgca ccgacgacat atgctgcagt
     4021 ataagaagtt gcttcgaaag attttgaaaa atgcagactg ggcatggagg tagcagcgtg
     4081 gaagcgatcg atggattgac agagcagttg gcgtcggcgg cgacgttgat cgaggcggat
     4141 gacctgagcg cgctcgccga gcttcacggg tttttccaac agctcgaccg ctggttcgag
     4201 gcacatcccc aagggcatcc gcgccatcag cagattcgcc aggccagcac gcaagcgcag
     4261 ggtgtgctcg aaaagctggt gcttcacgaa gcggacgacg tggacgcggc ggtgaatcag
     4321 gtcctggcgc tggtgagcga gatccaacga agcgcatccc tggcagccga atccgaacaa
     4381 ccggcgacgc aggcgccgaa tccggtggtg accgcggcac ccatcatgcc aacaccggcg
     4441 ccagcagccg aagcactaca ggccgagaaa ccggtggcgg tggacgacct gccgctggtg
     4501 cgcgacttca tcgctgaatc gatgtcgcac atggaatcgg ccgaagcggc gctgttgaag
     4561 attgaagaag ccccggacga catcgaagcg atcaattccg ttttccgcag ctttcacacg
     4621 atcaagggaa gcgccgggtt tttgaacctc aagcaaatcg gcgctctggc gcacgcgacg
     4681 gagaacctgc tggatttggc gcgcaagggg aagctgtcgc tggtgggtga cgtggcggac
     4741 gtggtgctgg aatcgctgga tgcgatgaag ggcctcatca tgacgctgcg cgacacggcg
     4801 gaggcaggac aggccgcccc gtcggaaccg cgcgtgccgg agctgatcca acggctgcac
     4861 gcggcggcgc gcggggaagc ggtgaagacg cagccgaagg cagcggcggc gcccgcgccc
     4921 gcgccgcaga cgacaggagc ggcagcgcca gcaactcaac cggtgaagcc cgcgccgcag
     4981 cccgcggcgt cgaaagatgc cggcgccgat gcgaccgtca aagtctccac cgaacgtctg
     5041 gatagtctga tcaatcaggt cggcgaattg gtgatcgcgc agtcgatggt gagccaggac
     5101 atggccgcca ccgcgcgggc gaatcccaag cacgcgcgca atctctcaca cctgggcaag
     5161 atcacgcgcg aaatgcagga tctggccatg tcgatgcgca tggtgccgat ccagggcgta
     5221 ttccagaaga tggcccgcgt cgtccgcgat ctgaccaaga aagccggcaa ggaagtcgat
     5281 ttcgtcacgg tcggcggcga gacggagctg gaccgcaacc tggtcgattc gatcagcgat
     5341 ccgctcattc acatggtgcg aaactcgtgc gaccacggca ttgaaatgcc cgatgcgcgg
     5401 gtacagtcgg gcaagccgcg tgccggccga atcgagctgc gcgcgttcca tcaaggcggc
     5461 agcattgcga ttcaaatcat cgacgacggc aaggggctgc ccaaggcccg catcctgcaa
     5521 aaggcgatcg acgcggggat cgtcaagccg gggcaggagc tgagcgagca ggagattttc
     5581 aagctgatct tccacgcggg tctttccacg gcggagaagg tgacggacgt ttccgggcgc
     5641 ggcgtgggga tggacgtcgt caagcgcaac gttgaagcgc tgcgcggccg catcgacatc
     5701 acctccaccg aaggccaggg ttcgacgttc acgatccgcc tgccgctcac gctggccgtc
     5761 atcgaagggc tcgtcgtgaa ggtcggcagc cagcgataca tcatcccgat caccagcgtc
     5821 gagcagacga ttcaaccgaa gcgcgaccag ctgtccaccg tgcagggccg cggggaaatc
     5881 tgcctcgtgc gcggcgcgcc gctgccactg tatcggctgc acaagatttt caacatcgag
     5941 ccgcgcacgc gcgacgtgac cgaagcgctg gtgctgatcc tgcacgacaa cgaccggcgg
     6001 tgctgcctgt tggtggacga attgctcggg cagcagcagg tggtcatcaa gagcatgggc
     6061 gaatcgctgg ggaccgtgcg cggcatcagc ggagcggcca tcctgggcga cggcagcgca
     6121 tcgctgatcc tggatgtcgc cggcatcatg gatatcgcgg ttgcgtgacg tgcgcaatcc
     6181 gtgactgagt ttcaggggaa gcacgcttaa tgaggagtgc acacgccgtt gcaggcgagt
     6241 gcacaggaag tgccaaatga gtttgaccat tggacgacgg ctaacgatcg ggtttgggtt
     6301 gtgcgttgcc gccacggctg cgttgggctt tttcatcaac aagcagctgt cggcgatctc
     6361 gcagaacgtc gggcatatta cgagtcacgc gctgccgggc gctgccatcg cggcggatat
     6421 cgacgcgatg gtcaagacca acgtcgtatt gctgctgcgg cacgtcacga cctcggatgc
     6481 agcggaaatg tcgcgtctgg aaggcgagtt taccaagacg aaggcgcacc tggatgaact
     6541 gttcgcggaa taccaggaat tgtcgcccgt gggtgacgaa ggtgaagcat ttgggcgatt
     6601 ggtccaggcg cgcaagggat ggttaagcgc caaggaaatg gtgatcacac ccagtcgcgc
     6661 aggtcagaag aaggccgctg ccgaagtctt cgcaacgcgc gccgttccac tgatcgatgc
     6721 tatcaatgat gccgcgcacg ggttactcga agccaaccac accagcggaa acgccgaggg
     6781 cgcgcaatcc gttgccacca tcgtcacggc ccgccgggca atcatcggcg gcatcaccgc
     6841 agcggcggtc ctcgcgctgg tcgtagccac tttaatcatt cgttcgacca accgctcatt
     6901 gcgcggcatg gccggcagcc tggtcaccgg ctcggaacag gtggccagtg catcgacgca
     6961 attgtcggct gcatctcagt cgctcgcgca aggcgcaagc gaacaggccg cggctttgga
     7021 ggaaaccagc gcggccctgg aagagatgag cagcatgacc aagaagaacg cggagaccgc
     7081 gaccaaagcc agcgccctgt cgaccgaggc gcacgaggcg gctactcgcg gcaacgcggc
     7141 gatgcagaag atgagcgcgg cggtgcagga catccagaaa tcctccggtg agaccgcgaa
     7201 gatcatcaag gtgatcgacg agatcgcttt ccagaccaac ctgctggcgt tgaacgccgc
     7261 ggtggaagcc gcccgggcgg gtgaagcggg tcgtggcttt gccgtggtcg ctgaagaagt
     7321 ccgcagcctt gcgatgcgct cggccgaggc agccaagaac acaacggcgc tgatcgagca
     7381 gagcgttcag aatacgcgga acggggtgac gatcgtcgac gaggtgggca agaacctcca
     7441 ggacatcaac gccgcctgca cgggcgtgaa gtcgctggtc ggggagatcg cggcggcttc
     7501 gacggagcag gcgcgcggga ttgaacaggt gaacaccgcc gtctcgcaga t
//
LOCUS       DATIMH010000700         5160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3633632, whole genome shotgun sequence.
ACCESSION   DATIMH010000700 DATIMH010000000
VERSION     DATIMH010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3633632"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..549)
                     /locus_tag="VLI90_12285"
     CDS             complement(46..549)
                     /locus_tag="VLI90_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15028.1"
                     /translation="MSWEAFRLTSKSPGELLHTLGPHGVDDLMRQALNAVWRDYPPEQ
                     RSYGAVRAAAGEVFNRNMRVWSAIKKPTPEAFFVDLQPQQADGFMRQAFVLCWMMLPR
                     TGGRDFKSTHAILRAMFQRNMDAWEQDNQTFTGGGGGKKRAKAKKATQPVKKKAAKKV
                     AKKTKRR"
     gene            complement(612..1556)
                     /locus_tag="VLI90_12290"
     CDS             complement(612..1556)
                     /locus_tag="VLI90_12290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSV15029.1"
                     /translation="MARLRELVSAMRSTGPLRFVFSVWREIGADNLFTLAAALAYSWL
                     FAIFPFLIFLLTLVAYVPHRYKVNAHDYLEGAVNRIMAKGAADTIMSNLDRVMKEPKT
                     GLLSIGAVLTLWAASGGVAMTMSALDAAYDAPTVRPFYKQRPIAIVLTFVLTALVLLV
                     LVLLPVGTTVLRWIAVHTHVPTAVIVAINVTRYGMALLLMFCVLAALYRFGTNVKQRF
                     TFISPGAVFTVVVWVLLGSTFRFYVDKFGHYDKTYGTVGGVAIILLFFYLDALVMLIG
                     AEINSEVDVAIKREAERQIAAESPEPHLPTALQTKASA"
     gene            complement(1709..3256)
                     /locus_tag="VLI90_12295"
     CDS             complement(1709..3256)
                     /locus_tag="VLI90_12295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV15030.1"
                     /translation="MADPTDPPLRVQAAGMRAVDLVLIVVVLSIAAAVRLSWLGSPGY
                     SVDELWTAELAVGRGSTHLHLPLNVLVPPPPPDLFRISDAPPWWHVWTHMECTHPPLY
                     FVALRLWIAAFGQGDVTERMLSVVCSMLAIALLYDAARLHNGRAVAFWAATLMALAQP
                     QIDLSRQTRNYAMLELTALAAIDALVRIEHFGINGRRCIALTAAVLATLLTHYFCIGA
                     VGAMGLYAMIRLPGRARSRVFIAFIIAGIVFAIGWGPFMWRQRALFATDDTATRFLQE
                     SGPQHLWLTLQRVTLIPAAMLAKPRLAMVVVSSGMVVLYVLPLLLLRRRPDLLLWCVL
                     LIGVVAPLVLLDLARGTGHLAFVRYPLLAGPAVYVLVPGLMTIAPTRWVCHAIPAAAA
                     IACALAAPDVYHPRQTDTRLMAAEMGSRIRAEDLLVFGGEGNHLWEAQAQYMMLSRYQ
                     RSIRSPMALVDRPVDGQVLDRARRSRYVFAFSVGNPLQFLPGFRLVDSRLYVGGGGCA
                     QLAPPAR"
     gene            3500..4915
                     /locus_tag="VLI90_12300"
     CDS             3500..4915
                     /locus_tag="VLI90_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSV15031.1"
                     /translation="MSSAPITPPPAAEPPPTSNAELGTAKIAVFTRTTDARSLLHLLD
                     DPRLQLFIAGPASSDGVRHANIRFVAGLDNPARGADSKAAGRLAIDAGADLVVVLTGD
                     EVDAVAAVRAVVDEFVSTRGDVTVHGSPEPPRGLIARSLLHLADNLAGRPLWLGGCRG
                     YSTAFLLSVPFELNRDDCVFDAEIVLQAAHVGATIRQVPSFGARAGGAACSAWQVLAV
                     AAQFRMHRMGMLCALKYRHLTPRRYQDKTFMLYTSHAMALAQVQRARPATLLDIGSGP
                     GFIAARCEAAGVRVTGIDVHEPLPGTMSEFHRVDLERDPLPVDAFAYDCVLMLDVLEH
                     MADPESFLLGLRNQSLALRTSDRQPLVIISTPNVAFAAVRLNLLLGRFNYAERGILDI
                     SHKRLFTRSTLRRALIDCGYTIERLIPIGPPFQTVVGGSVGKLLGKISDVMARAWPTM
                     FAFQFIVTCRPRPGVRVMVRE"
BASE COUNT          970 a         1751 c         1571 g          868 t
ORIGIN      
        1 ttctctcgga agaaaccgac gcttagccgc aagcagcggg gggaatcatc gccgctttgt
       61 ctttttcgcg accttcttcg ccgccttctt cttcaccggc tgcgtcgcct tcttcgcctt
      121 cgcccgtttc ttcccaccgc cgccgcccgt gaatgtctgg ttgtcctgct cccacgcgtc
      181 catgttccgc tggaacatcg cccgcaaaat cgcgtgcgtc gatttgaaat cccgcccgcc
      241 ggtgcgcggc agcatcatcc agcacagcac gaacgcctgc cgcatgaagc cgtccgcctg
      301 ctgcggctgc aggtcaacga aaaacgcctc cggcgtcggt ttcttgatgg ccgaccacac
      361 ccgcatgttg cggttgaaga cctcacccgc cgccgcgcgc acggcaccgt acgagcgctg
      421 ctccggcgga taatctcgcc acaccgcgtt cagcgcctgc cgcatcagat catccacccc
      481 gtgcggaccc agcgtgtgca gcaactcccc gggcgacttg ctcgtcaatc gaaaggcttc
      541 ccatgacatg cttgtattgt aattgaggtg attgcagaga aaagcgcacg atttcccaac
      601 gcgcccccgc cctaagccga cgccttcgtc tgcagcgccg tcggcagatg cggttccggc
      661 gactccgcgg cgatctgccg ctcggcctcg cgcttgatcg ccacgtctac ttcgctgttg
      721 atttccgcgc cgatcagcat caccagcgcg tccaggtaga agaacagcag gatgatcgcc
      781 acgccaccga cggtgccgta ggtcttgtcg taatggccga acttatcgac gtagaaccgg
      841 aacgtgctgc ccagcaagac ccagacgact acggtgaaga cagcgcccgg cgagatgaac
      901 gtgaaccgct gcttcacgtt tgtcccgaac ctgtacagcg ccgccagcac gcaaaacatc
      961 agcagcagcg ccattccgta gcgggtcacg ttgatcgcca cgatgaccgc ggtcggcacg
     1021 tgagtgtgca ccgcgatcca ccgcagcacg gtggtgccga ccggcagcag aaccaacacc
     1081 agcagcacca gcgccgtcag cacgaacgtc agcacgatcg cgatcggccg ctgcttatag
     1141 aatggccgca cggtcggcgc atcgtatgcc gcgtccagtg cggacatcgt catcgcgaca
     1201 ccgccgctgg ccgcccacag cgttagcacc gcgccgatgc tgagcagccc cgtcttcggt
     1261 tccttcatca cgcgatcgag attgctcatg atcgtgtcgg ccgcgccctt ggccatgatg
     1321 cggttcacgg ctccttcgag gtaatcgtgg gcgttcacct tgtagcgatg gggcacgtac
     1381 gcgacaagcg tgagcaggaa gatcaggaac gggaagatcg cgaatagcca cgaatacgcc
     1441 agcgccgccg ccagtgtgaa gaggttgtcc gcgccgatct cgcgccagac gctgaatacg
     1501 aagcgcagcg gaccggtgct gcgcatcgcc gaaaccagtt ctcgcagacg agccatgcga
     1561 caaagtggta agcggccccc ggcaaaaacg caaacgattc agagagaccc atggcagccg
     1621 ctggcgaaag ccagcgggtt tgtccgagct tcgagcggcg tggttcgtcg agcgcccccg
     1681 ctgacttgcg cctgcggcta ccaggccgtc agcgcgccgg tggcgccagc tgcgcgcacc
     1741 cgccaccacc cacgtacagg cggctgtcca ccaggcggaa acccggcaag aactgcagcg
     1801 gattgccgac gctgaacgcg aagacatatc ggctgcgccg cgcgcgatcg agcacttgac
     1861 catcaaccgg tcgatcgact aacgccatgg gcgaccggat cgaacgctga taccggctca
     1921 gcatcatgta ctgcgcctgg gcttcccaca ggtgattgcc ctcgccgccg aacaccagca
     1981 gatcctcagc gcggattcgg ctgcccattt ctgccgccat caggcgcgtg tctgtctgcc
     2041 gggggtgata cacatcgggc gctgccagcg cgcatgcgat cgccgccgcc gccgggatcg
     2101 catggcagac ccagcgcgtg ggggcgatcg tcatcagccc ggggaccaag acgtataccg
     2161 ccggcccggc cagcaacgga taccgcacga atgccaggtg gcccgtgccg cgcgccagat
     2221 ccaagagcac cagcggcgcg acgacaccaa tcaacagcac gcaccacagc agcaggtccg
     2281 gccggcgtcg cagcagcaac aacggcagca cgtacagcac gaccatcccc gacgaaacca
     2341 ctaccattgc cagccgcggt ttggccagca tcgccgcggg aatcagcgtg acgcgctgca
     2401 gcgtcaacca caggtgctgc ggccccgact cctgtagaaa tcgcgtggcc gtgtcatctg
     2461 tcgcaaacag cgcgcgctgc cgccacatga atgggcccca cccgatggcg aagacgatgc
     2521 cggcgatgat aaaagcgatg aacacgcgcg atcgcgcccg gcccggcagg cggatcatcg
     2581 cgtacagccc catcgcgccg acggcgccga tgcaaaaata gtgcgtcagg agcgttgcca
     2641 agaccgcggc ggtgagcgcg atgcagcggc ggccattgat gccgaagtgt tcaatgcgca
     2701 cgagcgcgtc gatggccgcc aaggctgtca gctccagcat cgcataattt cgcgtctgcc
     2761 ggcttagatc gatctgcggc tgcgccaacg ccatcagcgt cgccgcccaa aaggccacgg
     2821 cgcgcccgtt atgcagccgg gcggcgtcgt agagcagcgc gatcgccagc atcgaacaga
     2881 cgaccgacag cattcgttcg gtcacatcgc cctgtccgaa cgcagcaatc cacagccgaa
     2941 gcgcgacgaa gtacagcggc gggtgcgtgc actccatgtg cgtccacaca tgccaccacg
     3001 gcggcgcatc gctgatgcga aacaaatccg gcggtggcgg cggcaccagc acgttgagcg
     3061 gcaggtgcag gtgcgtcgat ccccggccaa ccgccagttc cgccgtccac agctcgtcaa
     3121 ccgaataccc cggcgatccc agccaagaca gccgcaccgc ggcggcgatg ctcagcacca
     3181 cgacgatcag caccagatcg acggcgcgca tgcccgccgc ttgaacgcga agcggcgggt
     3241 cggtagggtc tgccatacga ttggacgaat cgaccttgac gatcgcacga taatcagcgg
     3301 tcgatttcaa cgtgtcaatt tccggtgcgt gagcaggagc tggcggctgt cgtatcccaa
     3361 gccgggaggc cgcgtgatca acttgcaaat gcgcggactt cgaatcgatc ccccgccttc
     3421 tggcctgggc tatgacgaag ccgcgctaag ttcttagcga ctcgcacaag cgggtcatct
     3481 ggcgaagggc atcgaactcg tgtcatctgc gccgatcaca ccgccgcccg ccgccgagcc
     3541 accaccgacg tcgaatgcgg agttgggcac tgcaaagatt gctgtattca cgcgcaccac
     3601 cgacgctcgg tcgctcctgc atcttctcga cgatccgcgc ctgcagttgt ttatcgctgg
     3661 acctgcttca agcgatggcg tgcgtcacgc gaacattcga ttcgtcgccg ggctggacaa
     3721 tcccgcccgc ggtgccgatt cgaaagccgc cggtcgcctg gccatcgacg cgggcgctga
     3781 tcttgtcgtc gttttaactg gcgatgaagt ggatgcggtg gcggccgttc gcgccgtcgt
     3841 agacgaattc gtttcaactc gcggtgatgt cactgtccat ggatcgccgg agccaccgcg
     3901 cgggctcatc gctcgatcgc tgctgcatct ggcggacaac ctcgccggcc ggccgctgtg
     3961 gctcgggggg tgccgcgggt attccaccgc gtttttgctc agcgttccat tcgaattgaa
     4021 tcgcgacgac tgcgtcttcg acgccgagat cgtcctacag gcagcccacg tcggggcaac
     4081 gattcggcag gtgccgtcgt tcggcgctcg cgccggtggc gcggcttgtt ccgcttggca
     4141 agtcctggcg gtggccgcac aatttcgcat gcaccgcatg ggcatgttgt gcgccctcaa
     4201 atatcgccac ctcacgccgc ggcgatatca ggacaagacg ttcatgttgt acacctcgca
     4261 cgcgatggcg ctggcgcagg tgcagcgcgc acggccggcg acgctgctgg acatcggcag
     4321 cggccccggg ttcatcgccg cccgctgcga ggccgcgggt gtgcgcgtca ccggcatcga
     4381 cgtgcacgaa ccgctgccgg ggacgatgag cgagtttcac cgagtcgatc tggagcgcga
     4441 tccgctgccg gttgatgcct tcgcgtacga ttgcgtgctg atgctcgacg tgctggagca
     4501 catggccgac ccggaatcgt tcctgctcgg actgcgcaat caaagcctcg cgctgcgcac
     4561 cagcgatcgt cagccgctgg tgatcatttc gacgcccaac gtcgcgttcg cggcggtgcg
     4621 actgaacttg ttgctgggcc ggttcaacta cgccgaacgc ggcatcctcg acatcagcca
     4681 caagcggctg tttacccgat cgacgctgcg gcgagcgctg atcgactgcg gctacacgat
     4741 cgaacgcctg atcccgatcg gcccgccgtt ccaaaccgtg gtcggcggca gcgtgggaaa
     4801 gctgctcggc aagatcagcg acgtgatggc ccgggcctgg ccgacgatgt tcgcgtttca
     4861 gttcatcgtg acgtgtcgcc ctcgcccggg cgtgcgcgtg atggtgcggg agtgagagcg
     4921 gaatgtgaaa aaaaaacacg gctacgccgt gggacggaag gcaaaaaagc ttgtcccacg
     4981 gcatagccga gggctttgac tgtgtatctg cgtctcaatc acggcagctg ccagtgccgg
     5041 cgcaggaacc actcgcccac cagcagcaac atcaccatag ccccctgcat cggcaggtcg
     5101 tatcggccat tccaacccgg atctttcccg gcggcggcgg cgatggcgcg gatgtaattt
//
LOCUS       DATIMH010000701         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3633682, whole genome shotgun sequence.
ACCESSION   DATIMH010000701 DATIMH010000000
VERSION     DATIMH010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3633682"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..939)
                     /gene="mdh"
                     /locus_tag="VLI90_12305"
     CDS             complement(4..939)
                     /gene="mdh"
                     /locus_tag="VLI90_12305"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HSV15032.1"
                     /translation="MRRAKISIIGAGNVGATAAHWAAAKELGDIVLIDIPQVEGMPQG
                     KALDLYEASPIEGFDAKITGATTYEPSANSDVVIVTAGVARKPGMSRDDLININTGIV
                     KSVAENVAKHSPNAVMIVVSNPLDVMVYVAWKASGFPTKRVIGQAGVLDTARYRSFLA
                     AEIGCSVEDVQALLLGGHGDDMVPLARYTFAGGIPITQLVKKERLDQIVQRARDGGAE
                     IVKLLKTGSAYYAPAAATVQMAEAIIKDKKRILPCAAYCEKEYGIGGYFVGVPCVLGT
                     DGVERVIEIELDENERKMFQSSVDHVKELVKMVKL"
     gene            complement(1116..1565)
                     /locus_tag="VLI90_12310"
     CDS             complement(1116..1565)
                     /locus_tag="VLI90_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15033.1"
                     /translation="MKFGDHITWGTYGTRLHGDPRGTVDREHNERGAPALGFDEDRWE
                     HAKGNLKFPPVELTHEQRNFIEETIPIICVRGHWEHITSAARLDHVHTVLQSPFNPKT
                     VDAFEALAGSANGEALAAPAGADVLGGVRKHSADSESTVSDQRDGLC"
     gene            complement(1662..>4138)
                     /locus_tag="VLI90_12315"
     CDS             complement(1662..>4138)
                     /locus_tag="VLI90_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15034.1"
                     /translation="KTLITVGVDPQVNAMEYFGENPKTQAQLRPVVETVLKIYARAVD
                     EAGKRADAIANKLTPNDNAGIQRWQAADDLRNLAAFSGAMAQYDLCMALDKADPQRKR
                     TADDAIESLKQFDTNDSAVQPVVRNQIAKLNMVKGSFDAAKKMFDAIANNPNGEIKPA
                     PDKAQQYEARYFSVVCQILSRKIADAQKDMEALDTWQRANLTAEQQKGASAAFTMLKY
                     RVLSTRADLANDADDRRKNNEQAIATLMQLLKEQPQYKTVIFQQVLTRMPDNADVAKM
                     DPLLLLALQQQAELEYAKPEGQKLDEHALSRGIDAAREVIRRKAGNGLGAATVARSAY
                     VLPYLLERAPGREKEAAAAFLDYAQSYSSDTKNAGDSLDHATALIGRLRKQDLQDQDT
                     RKLYDRFLPIAINPPFNRKQFAFEYALLLQAEQHYKQAVETFKQVPPDDKRLLTAHFY
                     EMVALKQRLNDDDDKMSADERKSVVAEIQTLADEVNQKAAAAEAAATTPEDKKRYSSM
                     LVRTALLAADLANHEQKDPKRALDLLAGFEEKVKGQPNEQDLLFQALSLRVNSYMELG
                     QTNDATSTLVTLLKTKQGNEGPALVFDLLKKLDMDMEHARAAGDAAEVKQLAENRAAL
                     SGFLVTWAENNPDPKIKDLTNRYKAFDASTKQLAAELSDDPAKRKAGLEAALKQYQDL
                     YDANNPDPAVQLGMGFVQYDLGHYKQAKEALGPLVVNKKTGTPTVTITENGEPKTVES
                     SQYWEAILKLVRSIAEVAKADPNDAQAQKDLDAGKVFLKQQYVLWPKTIGGKKYHDDF
                     EKLRVELIPNFHPDTEEPASQPVAGS"
BASE COUNT          624 a         1326 c         1252 g          936 t
ORIGIN      
        1 tactcacagc ttgaccatct tcaccagctc cttcacgtga tcgacgctgc tttggaacat
       61 cttgcgttcg ttctcgtcca gttcgatttc gatgacacgt tcgacgccgt cggtgccgag
      121 gacgcacggg acgccaacga agtacccgcc gatgccgtat tccttttcgc aatacgccgc
      181 gcatggcagg atgcgcttct tgtccttgat gatggcctcg gccatctgca cggttgccgc
      241 cgcgggggcg tagtaggcgc tgccggtttt gagcagcttg acgatttccg ccccaccgtc
      301 gcgggcgcgc tggacgatct ggtcgaggcg ctccttcttg accagctgcg tgatcgggat
      361 gccaccggcg aaggtgtagc gggcgagcgg caccatgtcg tcaccgtgcc cgccgaggag
      421 cagggcttga acatcttcaa cgctgcagcc gatttcggcg gcgaggaagc tgcgatagcg
      481 cgccgtgtcg agaacgccgg cctggccgat cacgcgcttg gtcggaaagc cagacgcctt
      541 ccacgcgacg tacaccatca cgtccagcgg gttgctcacg acgatcatca ccgcgttcgg
      601 cgagtgcttc gcgacgttct ccgccacgct cttcacgatg ccggtgttga tgttgatcag
      661 gtcatcgcgg ctcatgccgg gcttgcgcgc cacgcccgcg gtgacgatga cgacatcgct
      721 gttggccgac ggctcatacg tcgtcgcgcc ggtgatcttc gcgtcgaacc cttcgatcgg
      781 cgacgcttca tacagatcga gcgccttccc ctgcggcatg ccttcgacct gcgggatgtc
      841 gatcaggacg atgtcgccca attccttggc ggccgcccag tgagcggcgg tagcgccgac
      901 attcccggcc ccgatgatgc tgattttcgc gcgacgcatg taaagccttt cgtaaagcgt
      961 taaggagcgg tgattatcaa aatgcaacgc gcgatttcaa ttatcgcgca actcgcttcg
     1021 tatcttgatg tcgctttatt tcgtgaccga gccgggccgt ccccggcccg ttctgcggaa
     1081 ttcacttcac gcgcggcccg ctcgttcgct gtttatcaac ataatccgtc ccgttgatca
     1141 gatacggttg attcagaatc ggccgaatgc ttccgcactc cgcccagaac gtctgccccg
     1201 gctggcgcgg ccaatgcttc gccatttgct gacccagcca acgcttcaaa agcgtccacc
     1261 gtcttcggat tgaagggcga ctgcaagacc gtgtgaacgt gatcgagtcg cgccgcggac
     1321 gtgatgtgct cccagtgacc gcggacgcag atgatgggaa tcgtttcttc aatgaagttt
     1381 cgctgctcgt gtgttagttc gaccggcgga aatttgaggt tgcccttcgc atgctcccaa
     1441 cggtcctcgt cgaaaccgag agcaggtgcg ccacgttcat tgtgttctcg atcgacagtg
     1501 ccacgcggat cgccgtgcag gcgcgtgcca tacgttcccc acgtgatgtg atcgccgaat
     1561 ttcatcgcgg ctgctcctgt ttcgaggtga aaagaacggg ccggggacgg cccggctcgg
     1621 tcacgaaatg acgggggccg cgagaaatga tggcggttcg ttcacgatcc tgcgacgggt
     1681 tgcgacgctg gttcttcagt gtcgggatga aaattcggaa tcaactccac gcgaagcttc
     1741 tcgaaatcgt cgtgatattt cttcccgccg atcgtcttgg gccacaggac gtactgctgc
     1801 ttcaggaaga ctttccccgc gtccaaatcc ttctgggcct gcgcatcgtt cgggtcggcc
     1861 ttggcgacct cggcgatcga tcgcaccagc ttcaggatcg cttcccaata ctggctgctc
     1921 tccaccgtct tgggttcgcc gttctcggtg atcgtcacgg ttggcgtgcc ggtctttttg
     1981 ttaaccacca gcgggccgag cgcttccttg gcttgtttgt aatggccaag gtcatactgc
     2041 acgaagccca tgcccagttg caccgcggga tcggggttgt tcgcatcgta caggtcctgg
     2101 tactgcttga gcgcggcttc caggccggcc tttcgcttgg cggggtcgtc gctcaactcc
     2161 gccgccagct gcttggttga cgcgtcgaat gccttatagc gattggtgag atctttgatc
     2221 ttcgggtccg ggttgttctc ggcccaggtg acgaggaacc cgctgagggc ggcgcgattt
     2281 tccgccagct gtttcacctc cgcggcgtcg ccggcggcgc gggcgtgctc catgtccatg
     2341 tccagcttct tgagcagatc gaataccagc gccggccctt cgttgccctg cttggtcttc
     2401 agcagcgtga ccagcgtgct ggtcgcgtcg ttggtctggc ccagctccat gtacgaattg
     2461 acccgcagcg acaacgcctg aaacagcagg tcttgctcgt tgggctggcc cttcaccttc
     2521 tcctcgaacc cggccagcag atcgagcgcg cgcttcggat ccttctgctc gtgattggcc
     2581 agatcagccg cgagcaacgc cgtgcgcacc agcatcgacg agtaccgctt cttatcctcc
     2641 ggcgtcgtcg cggcggcttc ggcggcagcg gctttttgat tcacctcatc cgccagcgtt
     2701 tggatttccg ccaccaccga cttgcgctca tccgcgctca tcttgtcgtc atcgtcgttc
     2761 agccgctgtt tgagcgcgac catctcgtag aagtgggcgg tcagcagccg cttgtcgtcg
     2821 ggcgggacct gcttgaaggt ctcgaccgcc tgcttgtaat gctgctccgc ctgcagcagc
     2881 agggcgtatt cgaacgcgaa ctgcttgcgg ttgaacggcg gattgatggc gatcggcagg
     2941 aagcggtcgt acagctttcg cgtgtcctgg tcctgcaggt cctgcttgcg cagccggccg
     3001 atcaacgccg tggcgtgatc gagcgaatcg ccggcgttct tcgtgtcgct cgaatagctt
     3061 tgcgcgtaat ccagaaacgc ggcggcggct tccttctcgc ggccgggggc gcgctccagc
     3121 aagtacggca gcacatacgc gctgcgcgcg acggtggcgg cgcccagccc gttgcccgcc
     3181 tttcgccgga tcacctcgcg cgccgcgtcg atcccgcgcg acagcgcgtg ctcgtccagc
     3241 ttctgaccct cgggcttggc gtattccaac tccgcctgct gctgcagcgc cagcaacagc
     3301 agcgggtcca tcttcgccac gtcggcgttg tccggcatgc gcgtcagcac ctgctggaaa
     3361 atgacggtct tgtattgcgg ctgctctttc agcagctgca tcagcgtcgc gatggcctgc
     3421 tcgttgttct tccggcgatc gtcggcgtcg ttggccagat ccgctcgcgt tgagagcacg
     3481 cggtacttca gcatcgtgaa cgcggccgac gcccccttct gctgctcggc cgtcaggttg
     3541 gcgcgctgcc aggtatccag cgcctccatg tccttctgcg catcggcgat cttgcgcgac
     3601 aggatctggc agacgacgct gaaatagcgg gcttcgtatt gctgcgcctt gtccggcgcg
     3661 ggtttgatct cgccgttcgg attgttggcg atcgcgtcga acatcttctt cgccgcgtcg
     3721 aagctcccct tcaccatgtt cagcttggcg atctggttcc gcacgaccgg ctgcacggcg
     3781 ctatcattgg tgtcgaactg cttgagcgat tcgatggcgt catcggcggt ccgcttgcgc
     3841 tgcgggtccg ccttgtccag cgccatgcac aggtcgtact gcgccatcgc gccgctaaac
     3901 gccgcgaggt tgcgcaggtc gtccgccgcc tgccagcgct ggatgccggc gttgtcgttg
     3961 ggcgtgagct tgttggcgat cgcatcggcg cgcttgcccg cttcatcgac ggcgcgggcg
     4021 tagatcttca gtaccgtctc gaccaccggc cgcagctgcg cctgcgtctt ggggttctcg
     4081 ccgaaatatt ccatcgcgtt gacctgcgga tcgacgccaa ccgtgatcag tgtcttgg
//
LOCUS       DATIMH010000702         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3343524, whole genome shotgun sequence.
ACCESSION   DATIMH010000702 DATIMH010000000
VERSION     DATIMH010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3343524"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VLI90_12320"
     CDS             complement(<1..412)
                     /locus_tag="VLI90_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15035.1"
                     /translation="MRVKANVVVGSLALVVLCGVLVSCAPDKPAVEDRTDLLAQRGGA
                     AGAKSGQTSSASDMPVPPKDALYTIYCQILSGPDHIERSRQLRLALRNSTSLKDWYLI
                     HGADQSTLYYGFYRTIDPRDSKDADEGKRAIHDLN"
     gene            complement(542..1321)
                     /gene="rph"
                     /locus_tag="VLI90_12325"
     CDS             complement(542..1321)
                     /gene="rph"
                     /locus_tag="VLI90_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HSV15036.1"
                     /translation="MRQDGRANDELRPITVQRRFTETPAGSVLWRQGSTIVLCTAAVT
                     PEVPPWFAVDRAGGWITAEYVMLPASTPQRKPWPKIGHTDSRGTEIQRLIGRVLRAAI
                     DLEKIGPHTIALDCQVLQADGGTRTAAICGAYVALVDALAKLPAEIPPPRNPADRTKF
                     AARYDASRYKPQQAIVAPLAAVSVGIIDGEERLDLDYVDDSRAEVDMNVAYTAKGEFV
                     EVQGAAENGGGFDRGRMNRLLDLAVAGCERLMKVQREALGQ"
     gene            1574..2425
                     /locus_tag="VLI90_12330"
     CDS             1574..2425
                     /locus_tag="VLI90_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15037.1"
                     /translation="MARIALLLALMLSLSTMAAAPSTQPTTAPMAVDVLVLPFTPIVA
                     ESASDFLSHAVQQNLITDLGRGQYHPTAGDPTTATPLAAGRDTGARYVIAGSYQSIEG
                     QLRFTGQIVDVQAGTVVGGMTATGGPRDLFAIEDSLSAQALHQLQRLNTQSTAAAHGK
                     PAAAVQAPAPAPAIAAPAQPAAPVFMRTFEGSELQSYANSNRTPSQDYAAQVAASANR
                     QAYWPTGYSGYGSYGGYGYYSYGYPWSVGYTYPYGFAYGYTTPFVAVRMTPQRATVYG
                     NNYSQQP"
     gene            complement(2884..>4096)
                     /locus_tag="VLI90_12335"
     CDS             complement(2884..>4096)
                     /locus_tag="VLI90_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV15038.1"
                     /translation="TTGDTVVDSGTMQLNNSAAATGTIFIGNGGSTATPATLNVGLSG
                     LNIPNTIQTNIDAGGASNIRTIAGTYASGSSTISGPINVNGGLTIGATNAAATLAVTG
                     AIADGADGSAGISHNVTINGGGNVLLSAANTYTGNTTVSSGTMTVDTTGSLASPNVTV
                     ASGATMNVNGALAPSANVTANGLAVFAGNHTANATLPIPLGTLNIGAGGIVRLADSDF
                     AARPSVMQPGSLTIDAAGKLDVMNNEVVFPGTAGGALTMIQAGQIVSTESPADPSKAL
                     GYIDLSGADAGKVEVRYTLKGDTNLDGIVNVGDLGALATNYNITGGMSWANGDFTQDG
                     TVNVADLGALATNYNNHLANGAASGAVAQSLAIVASGGGGATAVPEPGALSLVGLLAS
                     SLLTRRRARRS"
BASE COUNT          673 a         1404 c         1264 g          755 t
ORIGIN      
        1 tgttcaggtc gtggatcgcc cgcttgccct cgtcggcatc cttcgaatcg cgcgggtcga
       61 tcgtccgata gaagccgtaa taaagcgtgc tctgatcggc cccgtggatc aggtaccagt
      121 ccttcagcga cgtgctgttg cgcagcgcca gccgcagctg gcgcgagcgc tcgatgtggt
      181 ccggccccga caggatctgg cagtagatcg tatacagcgc atccttcggc ggcaccggca
      241 tgtcactggc cgaggatgtc tgcccgctct tggcgccggc agcgccgccg cgttgtgcga
      301 gcaaatcggt gcgatcttcc accgctggtt tgtccggcgc gcacgatacg agcacgccac
      361 acaacaccac cagcgccaac gaaccaacaa ccacgttcgc ttttacacgc attgccaaag
      421 ctctccgcat gatcactgac ccccccgacg ccggtgtctg aggtactccg aagacccgga
      481 tcgttcgcgt gaccaatccg ggtcttcgga gtacctcaga caccggcgtg agcgcgaacc
      541 ctcattgtcc cagcgcctcg cgctgcacct tcatcaaccg ctcgcacccc gcgacggcca
      601 ggtccagcag gcggttcatt ctaccacgat cgaacccgcc accgttctcc gccgcgccct
      661 gcacttcgac aaattctccc ttggccgtgt acgcgacgtt catgtccacc tcggcccgcg
      721 agtcgtcgac gtaatccagg tccagccgct cttcgccatc gatgatgccg acactcaccg
      781 ccgccagcgg cgcgacgatc gcctgctgcg gcttgtatcg tgacgcatcg tatcgcgccg
      841 cgaacttcgt tcgatccgcc gggttccgcg gcggtgggat ctccgccggc agcttcgcca
      901 gcgcatcgac cagcgcgacg tacgccccgc agatcgccgc cgtgcgcgtg ccgccgtcgg
      961 cctgcagcac ctggcaatcc agcgcgatcg tgtgcggtcc gatcttctcc aggtcgatcg
     1021 ccgcccgcag cacccggccg atcaaccgct ggatctcggt cccgcgcgaa tcggtatgcc
     1081 cgatcttcgg ccagggcttg cgctgcggcg tcgatgccgg cagcatcaca tactccgccg
     1141 tgatccaccc acccgcgcga tccaccgcaa accacggcgg cacctccggc gtcaccgccg
     1201 ccgtgcacag cacgatcgtg ctgccttgcc gccacagcac cgaccccgcg ggggtctcgg
     1261 tgaatcgccg ctggactgta atgggccgca gttcatcgtt cgcccgacca tcttgccgca
     1321 tggggcaacc gtaagtgcga cgacctatcg acgcaagacg agccgtcgcc tccaaccggc
     1381 gatgcacgat tatgcacgat tgctaaggtg cgtgcacgga aggtaagatc acgccaatac
     1441 ggttgttgcg accgatttag caatattatg atgcaccgga tcgtgcatcg acattctttc
     1501 cgcagcacag catcgccacg cgtgtctata ctgatgttcg ccgctgcgct tcgcgcggta
     1561 cacttggtga gccatggcga gaatcgcact gctcttggcc ttgatgttgt cgctgtcaac
     1621 gatggcggcc gcgccatcca cgcaaccgac caccgcgccg atggccgttg atgtgctcgt
     1681 gctgccgttc acgcccatcg tcgccgaatc ggcgtccgac ttcctcagtc acgcggttca
     1741 gcagaacctg atcaccgatt tgggccgggg gcaataccat ccgaccgccg gtgacccgac
     1801 gaccgccacc ccgctcgccg ccggtcgaga cactggtgcg cgctacgtca tcgccggctc
     1861 gtatcagtcg atcgaaggcc agcttcgatt caccgggcag atcgtcgatg tgcaggcggg
     1921 cacggtcgtc ggtggcatga ccgcaacggg cgggccgcgc gatctattcg cgattgaaga
     1981 ttcgctgtct gcccaggcgc tacatcaatt gcaaaggttg aacacgcaat cgaccgcggc
     2041 cgcccacggc aaaccggcgg ccgccgtgca agcgcccgcg cccgcgcctg ccatcgcggc
     2101 gcccgcgcag cctgcggcgc cggtcttcat gcggacgttc gaaggctcag agttgcagtc
     2161 ctacgccaac agcaaccgca cgccgtcgca ggattacgcg gcgcaagtcg ccgccagcgc
     2221 caatcgccag gcttactggc ccaccggtta cagcggctac ggcagctacg gtggatacgg
     2281 ttactacagc tacggctatc cctggtccgt cggttacacc tacccgtacg gcttcgccta
     2341 cggctacacc acgccgttcg tcgccgtgcg catgaccccg caacgggcga cggtgtatgg
     2401 caataattat tcgcagcaac cgtgaatgat ctgcaggaac agacgggtct gaggtcggtc
     2461 agaagagaag cgaatggtgc cacggctgct ggtcgagcgc ccggcggatc gcgcatctga
     2521 aaagggactg aatgcttaat agcccaggcc ggaaggccgg ggatcggaca aatagctgcg
     2581 ccatttccag tccgatcccc ggcctcccgg cctgggctat tatggccaca tacttccaag
     2641 atttagaggt gctctgagca cagcacctac tcgtgcgcag tcgagcgatc tgtgccaggt
     2701 gtaagcgaac tctcactcgc gagtcccgcc gcttgcggct tagcgtcttt tggaattcga
     2761 acaaacgcta agccgcaagc ggctgtggcg cgctattcgt gaccgagccg ttcatcgact
     2821 tcccggtcca gatccttcga ctccggcgaa tgcgcctgcg ctggaggatg acagcgggcg
     2881 cgatcagctc cgccgcgcgc ggcggcgggt caacaggctt gatgcaagga ggccgaccaa
     2941 gctaagcgcg cccggttctg ggacggccgt ggcgccgccg ccgccgctgg cgacgatcgc
     3001 cagcgactgc gccaccgcgc cgctggctgc gccgttggcg aggtggttgt tatagttggt
     3061 cgccaacgcg cccaggtcag cgacgttgac ggtgccatcc tgggtgaagt caccgttcgc
     3121 ccagctcatc ccgccggtga tgttgtagtt cgtcgccagc gcgcctaaat cgccgacgtt
     3181 cacgatgccg tcgaggttcg tgtcgccctt gagcgtgtag cgcacttcca ccttgccggc
     3241 gtccgcgccg ctcaggtcga tgtagccaag cgctttgctc ggatccgccg gcgattccgt
     3301 ggagacgatc tggccggcct ggatcatcgt cagcgcacca ccggccgtgc cggggaagac
     3361 gacctcattg ttcatcacat ccagcttgcc ggctgcgtcg atcgtcagcg agccgggttg
     3421 catgaccgaa ggccgggcgg cgaaatcgga gtccgccagc cgcacgatgc caccggctcc
     3481 gatgttcaac gtgccgagcg ggatcggcag cgtcgcgttg gccgtgtggt taccggcgaa
     3541 gacggccaga ccattggcgg tcacgttcgc cgacggcgcc agcgcgccgt tcacgttcat
     3601 cgtggcgccg ctggcgaccg tgacgttggg gctggccagc gagccggtgg tatccacggt
     3661 catggtgccg gagctgacgg tcgtattgcc cgtgtaggtg ttggcagcgc tgagcagcac
     3721 gttgccgccg ccgttgatcg tgacgttgtg gctaatgccg gcgctgccgt cggcgccatc
     3781 ggcgatcgcg cccgtgacgg ccagcgtggc ggcggcgttg gtcgcgccga tcgtcaaccc
     3841 gccgttgacg ttgatcggcc cgctgatcgt cgagctgccg ctggcgtatg tgccggcgat
     3901 cgtgcggatg ttgctggcgc caccggcgtc gatgttcgtc tgaatcgtgt tcgggatgtt
     3961 caggccactg aggccgacgt tcaacgtcgc cggcgtcgcg gtgctgccgc cgttgccgat
     4021 aaagatcgtg cccgtcgccg cggcgctgtt gttcaactgc atcgtgcccg aatcgacgac
     4081 ggtgtcgccg gttgtc
//
LOCUS       DATIMH010000703         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4792307, whole genome shotgun sequence.
ACCESSION   DATIMH010000703 DATIMH010000000
VERSION     DATIMH010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4792307"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VLI90_12340"
     CDS             complement(<1..191)
                     /locus_tag="VLI90_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15039.1"
                     /translation="MRRHFHLKTRRLTAAAAAAALSLGSLGVAPAFGAFTFTKIVDSN
                     TADPGGAGTINSFSPPALDG"
     gene            complement(364..2544)
                     /locus_tag="VLI90_12345"
     CDS             complement(364..2544)
                     /locus_tag="VLI90_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSV15040.1"
                     /translation="MSGFAGEQTNGPSQRKTLHIGVDVGGTFTDLVAFDGETLRVVKI
                     PSTPPAFDRAVVEAVRRATAMFGDASQDVQIVHGSTVATNALLQRAGEPVAFITTEGF
                     RDMLLIGRQNRPKLYALRIERPPPITPAENWFTVRERIDAKGNVITPLEDAAIDQLVR
                     EIHHRHLKHAAVCLLFSFVNADHERRIGAALEAAGITVSLSSDVLPEFREYERASTTA
                     INASLRPTVEKYLRDLQDGLLAQHPHLARREHTPGCAANVAPLPLREESGAKSQVDPA
                     CVHAGLNGAFDASTPSLRIMHSGGGTLSVADASRYAAKLVLSGPAGGVIGAAFVAHAA
                     GFDDVITYDMGGTSTDVALVLNGEPQWSTGGVVDGLPIGLPAFDIHTVGAGGGSIAHL
                     DTGGALRVGPKSAGAVPGPACYARGGTLPTVTDANLCLGRILPDRFLGGGMTIEPSLA
                     TGAIEPLAKSMNKSVIEAALGIVKVAEANMASAIRAVTSQRGHDPRNFSLVSFGGAGG
                     LHACALADALEIPRVIIPPYCGVLSALGMVVAKPIADASKTVVQIAGQMDDTRLKIEF
                     EALDAIARQQMPDDAMARTERFADARFRGQSYEVKVPVETMSIAAIAERFKSAYNALY
                     GMLPQGREIELVTLRVRRVGHGVNVALPPITRSATVEPRDVSIITPDGVRTIAKAYTR
                     TALRQSTTTGPMLLIDPTATAYIPAGWRAKTQSTGVVLLNHRPG"
BASE COUNT          426 a          828 c          942 g          480 t
ORIGIN      
        1 ccatccagcg ccggcggcga gaaggagttg atcgtccccg cgccgccggg gtcggcggtg
       61 ttgctgtcga cgatcttcgt gaacgtgaac gcgccgaacg cgggggcaac gccgagcgac
      121 ccgagcgaga gcgctgcggc ggcggcggcg gcggtgagcc gacgcgtctt gaggtggaaa
      181 tgtcggcgca tgtgatccct cctgccctgt cagtcgatcc ggggaacggt tgtacgacgt
      241 gtttccccca ctagcccaaa ggacgcggaa acgtatcaaa gcgagaacaa tagtagaatt
      301 ttgggcggtt gtgtcaagga aaattcggtt ttttgcttcg gcgaacgcta accgctgaag
      361 cggttagccc ggtcggtggt tgaggagaac gacgcctgtt gattgggtct tggcgcgcca
      421 gccggcgggg atgtatgcgg tggcggtggg gtcgatcagc agcatcggtc ctgttgttgt
      481 cgattgccgc agtgcggtgc gggtgtacgc cttggcgatg gtccggacgc cgtcgggggt
      541 gatgatcgat acgtcgcgcg gttcaacggt ggcgctacgt gtgatgggcg ggagggcgac
      601 gttgacgccg tgaccgaccc gacggacgcg cagcgtaacc aactcgatct cgcggccttg
      661 cggcagcatg ccgtagagcg cgttgtacgc ggacttgaag cgctcggcga tcgccgcgat
      721 cgacatcgtc tcaaccggca ccttcacctc gtaggactgc ccgcggaagc gggcgtcggc
      781 gaaacgctcg gtgcgcgcca tcgcatcatc cggcatctgt tggcgggcta tcgcatcgag
      841 cgcctcgaac tcaatcttca gtcgcgtatc gtccatctgg cccgcgatct gcacgaccgt
      901 cttcgacgca tccgcgatcg gtttggcgac gaccatgccc agcgcgctga gcacgccgca
      961 gtatggcggg ataatcacgc gcgggatttc cagggcgtcg gccagggcgc aggcgtgcag
     1021 gccaccggcg ccaccgaagc tgaccagcga gaagtttcgc gggtcgtggc cgcgctggct
     1081 ggtgacggcg cggatggcgc tggccatgtt cgcttcggcg actttcacga tgccgagggc
     1141 ggcctcgatc acgctcttgt tcatcgactt ggccagtggt tcgatcgcgc ccgtggcgag
     1201 gctgggttcg atcgtcatcc cgccgccgag gaaacgatcc ggcaggatcc ggcctagaca
     1261 taagttggcg tcggtgacgg tcggcaatgt gccgccgcga gcgtagcacg cgggaccggg
     1321 caccgcgccg gcggatttgg ggccgacgcg cagcgccccg ccggtgtcga ggtgggcgat
     1381 cgaaccgccg ccggcgccga cggtgtggat gtcgaacgcg ggcaggccga tgggcaggcc
     1441 gtccacgacg ccgccggtgg accattgcgg ttcgccgttg agcacgagcg cgacatcggt
     1501 gctggtgccg cccatgtcgt aagtgatcac gtcgtcgaag ccagccgcgt gcgcgacgaa
     1561 cgcggcgccg atgacgccgc cggccggacc acttaggacg agcttcgcgg cgtagcgcga
     1621 ggcgtcggcg acggagagcg tcccgccgcc ggagtgcatg atgcgcagcg aaggcgtcga
     1681 tgcgtcgaaa gccccattta gcccggcgtg aacacacgcc gggtcaactt gggacttcgc
     1741 gccgctttcc tcgcgcaagg gaagcggcgc gacgttcgcg gcgcaacccg gcgtgtgttc
     1801 acgccgggct aaatgggggt gttgcgcgag caggccatcc tgcaagtcgc gcaggtactt
     1861 ctcgaccgtc gggcgcagcg acgcgttgat cgcggtcgtg ctggcgcgct catactcgcg
     1921 aaactccggc aggacgtcgc tgctcaaact caccgtgatc cccgcggctt cgagtgcggc
     1981 gccgatcctt cgttcgtgat cggcgttgac gaagctgaac agcaaacaca ccgccgcgtg
     2041 tttgaggtgg cgatgatgga tttcgcgcac cagctggtca attgccgcat cttccagcgg
     2101 cgtgatgacg tttcccttcg cgtcgatccg ctcgcgcacc gtgaaccagt tctccgccgg
     2161 cgtgatcggc ggcgggcgtt cgatccttag cgcgtacagt tttggccggt tctgccggcc
     2221 gatcagcagc atgtcgcgga agccttcggt ggtgatgaat gcgacgggtt cgcctgcccg
     2281 ctggagcaag gcgttggtcg cgacggtgga accgtgcaca atttggacat cttgcgacgc
     2341 gtccccgaac atcgccgtcg cccgccggac ggcttccact accgcgcggt cgaacgccgg
     2401 cggcgtggag gggatcttca cgacgcgaag cgtttctccg tcgaacgcca ccaggtcggt
     2461 gaacgtcccg ccgacgtcga cgccgatgtg aagcgtcttt cgctgagatg gcccgtttgt
     2521 ttgttcgccc gcgaaaccag acatacaatg cgatccgatg cgtaattcct gcgtctcccg
     2581 gcgtctgggc tggacgttac tcgcggtact gctgggctgc aattcggcgc tgagcggcgc
     2641 cgaggcgctg aaccagtcgg cgccgacgac gaaggc
//
LOCUS       DATIMH010000704         1944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1904913, whole genome shotgun sequence.
ACCESSION   DATIMH010000704 DATIMH010000000
VERSION     DATIMH010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904913"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VLI90_12350"
     CDS             complement(<1..641)
                     /locus_tag="VLI90_12350"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSV15041.1"
                     /translation="MPPVNVVLIGPPGSGKGTQAARLAERYAIPHISTGEILRSAVRA
                     GSDLGRQVADTLAKGSLVSDDLMTDLVRVRLKEPDVARGFVLDGYPRTVGQAHALDEM
                     IGRAPMIVILIEVPDGEIVRRMGTRRVCDSCRLTQSVADAFHPDSEPCPYCGGNLVRR
                     PDDEPETVRHRLATYAQYATPVIEHYRSCPAFGVVDGVQHADYVTAAMTAHIDR"
     gene            716..>1944
                     /locus_tag="VLI90_12355"
     CDS             716..>1944
                     /locus_tag="VLI90_12355"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSV15042.1"
                     /translation="MTWDVHWAYDPRPNRPRANIADPPGGAVNILLLSMPDSFEHTPT
                     LTIRMPNGALASLAGNVDAHHRVAIADLILAQSSVRQTIERLVADVCPDVVGLSVMTF
                     QRSTARRIIALVRSLAPKVRIVVGGYDPSLAPEAWTDPDIGVDAIVRGEGDLTFRELI
                     RAFEGRQPLSSVAGLWFRDGQSFRTTPQRAVTHLDAGEIRPPNRAARVLSGYTMMGRQ
                     IDVIETSRGCTFDCSFCSIIEMRGRNFHRFPIERVIDDIRDAEARGARSIFIVDDNIT
                     LDVERFEELCTAIVRAGLNRIDYLVQGMTASIATHGKRLAPLMREAGFRYVFLGIENI
                     LDEDLAFLKARSKNSRHQSGLRTNTAVAAVDILHEHGMLVVGGLIVGNPDDRRESIAA
                     NLEFARKYVDWPYIQHPT"
BASE COUNT          345 a          635 c          610 g          354 t
ORIGIN      
        1 cgatcaatgt gagccgtcat cgcggcagtc acgtagtccg cgtgctggac accgtccacg
       61 acgccgaacg ccggacacga gcggtaatgc tcgatcaccg gtgtcgcgta ttgtgcgtag
      121 gtcgcgagcc gatggcgcac ggtttcgggt tcatcgtcgg gtcgccggac aagattgccg
      181 ccgcaatatg gacacggttc gctgtcggga tgaaaggcat ccgcaacgga ctgcgtcagc
      241 cggcagctat cacacacgcg acgcgtgccc atccggcgga caatctcccc gtcagggacc
      301 tcgatgagga tcacgatcat cggcgcgcgc ccgatcattt cgtccagcgc gtgtgcctgc
      361 cccaccgttc gcggatatcc atccaggacg aatccgcgcg ccacgtcagg ttccttcaat
      421 cgcacccgga cgagatcggt catcaggtcg tcgctgacga gcgacccctt ggccagcgtg
      481 tcggccactt gccgccccag gtcgctgccg gcgcgaaccg cagatcgcag aatctcgccg
      541 gtggaaatgt gcgggatcgc gtaccgttca gccagccggg cggcttgagt tcccttcccg
      601 ctgccgggtg ggccaatcag cacgacgttc actggcggca tcgatcgtgt gccccctgca
      661 gtggtggtcg cgccaggctc ggcatgtggg ctgtccaacg acacgcccgg acattgtgac
      721 atgggacgtg cattgggctt atgatcctag gccgaatagg ccgcgtgcga acattgccga
      781 ccctcccgga ggcgccgtga acattctgct cctctcgatg cccgattcat tcgagcacac
      841 gcccacattg accatccgta tgccaaacgg agcgctggct tcgctcgccg gtaacgtcga
      901 cgcgcaccat cgggtcgcca tcgcggatct gatcctcgcc cagtcgtcgg tgcgacaaac
      961 gatcgagcgg ctcgtcgccg acgtctgtcc ggacgtggtc ggtttatcgg tgatgacgtt
     1021 ccagcgatcg acggcgcggc gcatcatcgc gctggtgcgg tccctcgcac caaaggtccg
     1081 catcgtggtc ggcgggtacg atccgagcct ggcgccggaa gcgtggacgg atcccgacat
     1141 tggcgtcgac gcgatcgtca ggggcgaggg ggatctgacc tttcgagagt tgatccgtgc
     1201 cttcgagggc cgacagccat tgtcgagcgt cgcgggtctc tggttccgcg atgggcaatc
     1261 gttccgaacc acgccgcagc gtgctgtgac ccacctcgac gccggggaga tccgtccgcc
     1321 caaccgcgcc gcccgcgtgc tgtcggggta cacgatgatg ggccggcaaa tcgacgtcat
     1381 cgagacgtcg cgcggctgca ccttcgactg cagcttctgc tcgatcatcg agatgcgcgg
     1441 gcgcaacttt caccgattcc cgatcgagcg ggtgatcgac gacatccgcg acgccgaagc
     1501 ccgcggcgcg cgcagcatct tcatcgtcga cgacaacatc accctcgacg tcgaacgatt
     1561 cgaggagctc tgtacggcga tcgttcgcgc cggattgaat cgaatcgatt acctggtcca
     1621 ggggatgacg gcatcaatcg cgacgcacgg gaagcggctg gcgccgttga tgcgcgaggc
     1681 cggatttcgc tacgtatttc tcggcatcga gaacatcctc gacgaggacc tcgcttttct
     1741 gaaagcgcga tcgaagaaca gccggcatca gtcgggactt cgcacgaaca ccgcggtggc
     1801 tgccgtcgac atcctgcacg agcacggcat gctggtcgtg ggaggcctga tcgtcggtaa
     1861 tcctgacgat cgtcgtgagt cgatcgccgc caacctggaa ttcgcacgga agtacgtcga
     1921 ctggccgtac atccagcacc caac
//
LOCUS       DATIMH010000705         6628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3057781, whole genome shotgun sequence.
ACCESSION   DATIMH010000705 DATIMH010000000
VERSION     DATIMH010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057781"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VLI90_12360"
     CDS             <1..447
                     /locus_tag="VLI90_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HSV15043.1"
                     /translation="KKHVTDIRGDRLKKPIDDIAGGRVYTGKQALALGLVDKIGTLSD
                     AIAFAADQAKMAADYDVRTVPEPQNVLQRMMEDANGGENDPSRVEVGSSPMRAEANPL
                     LKAAMPLLAGLDPQHVRMLCDALDQLQLVQREGVIMMMPPQQPWAR"
     gene            586..1911
                     /locus_tag="VLI90_12365"
     CDS             586..1911
                     /locus_tag="VLI90_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0051118 - glucan
                     endo-1,3-alpha-glucosidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,3-alpha-glucanase family
                     glycosylhydrolase"
                     /protein_id="HSV15044.1"
                     /translation="MRLPLVFGCALFSLIAITAHAATDDPFTFPETDLMRAAPRAVFV
                     AFMGDFHNPVGSGRWGAWAHTGEKNAPHDPGHIDPATGRRDIASCFYPSIGPYDQGDP
                     DVIDCQCQLLKMAGIDGISFNLSFYQLDEWRQRSMRMYVDAMKRYALLAIVRFENKFF
                     PDKYPDPTEMLAASYSDMDAWMKMIEPVQYRVSGRPVFMLFTFKLTPDQLRAWADRFP
                     VAQRPIILTYGADPKYKSVVDGRFGWTGDQPNRLKDHPPYVSYVTPELMRVNERRDRQ
                     RALELLESGQITFYMAGVAPGFDDIGCWGWGNGPRKVERDDGQTYQYRWQQTLKTNLR
                     NVFIPTWNDWNEGTTIEPSIEYGDQYITMTRQYAAQFKQTAPPSSGSLMLPIWIYKIR
                     KTTPDAAADMSRASELIVTGAFDQARALVEPWWKKLNLDDKTPWDRATQ"
     gene            complement(1954..3192)
                     /locus_tag="VLI90_12370"
     CDS             complement(1954..3192)
                     /locus_tag="VLI90_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HSV15045.1"
                     /translation="MGSSTRRTIAVVMATHMTCYRELLRGITAFANATASWELHHYLP
                     VDNLARFVEQDKPQGLLLGALSNAEQGRQAVACVQRCVGVCGFPAEDGIAVTMDFESD
                     DLRVGQVAARHFVQKGYREFAFVGRESSWSHQRGEGFRTEIESAGFGCSMLQQDSEST
                     ITGRGWDMIDRAGPLLDFLRVAPRPLALFACNDIRARSIAQMCRNEGIRVPDDVAILG
                     CDNDDLDCEVSDPPLSSVAVPWRRIGYDAAAALDRMLDGESIEPSVILLPPIGVVERQ
                     STDSVAVSDPDVAAAIRFIREHAHERTSVDDVLQAVPIGRRSLEKRFRAVLKRSPLEE
                     IRRVHVERAKHLLGTTDLSVNDVARHSGFGRSTWFTEAFGALVGESPAQFRKRYQRAT
                     VYTGGAMEPVPARAAEPMGS"
     gene            complement(3405..3803)
                     /locus_tag="VLI90_12375"
     CDS             complement(3405..3803)
                     /locus_tag="VLI90_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15046.1"
                     /translation="MGGTFATMLLITIGCTATSKVVFNGLPGAVLFLDGQPHHLPSDV
                     KFTRPASASGSTQHDADLAFTSSQSKEIRATGTLNAFAYTESDFDKVAVATCNLGEEQ
                     LVKIPNGTTLIFKGQTANRQALYKLTPKQK"
     gene            3959..5227
                     /locus_tag="VLI90_12380"
     CDS             3959..5227
                     /locus_tag="VLI90_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HSV15047.1"
                     /translation="MASRSAATRSAGILLAADAAMDSLPEQARPMDALRGQRVTDALS
                     AAGLDAVVCALPTNVLLLSGYWPIVGTGVIVAGRERRILLVPKDEQSLAERGHADEVR
                     TFEPGGSLTQMATAAESVRDPLADIATELGIERGRIGFEAGEWFEPASYAAMHLYAGA
                     MHRVLQSALPSATIEPADEMLRSLRTVKTPAELDRISAACAICQTAFTRGAGEVRAGV
                     SEVEAAARFRVPLASPLASRADGHVAFMSGINSASAYGAYARSTDKVMARGELVLVHC
                     NSYADGYWTDVTRTFCLGPPDERRRRMYDAVFAARDAAFKAIRPGARGADVDRAARDV
                     LAAAGFAAAFKHPTGHGVGFAAIDHNARPRVHPKSDDVLEAGMIFNVEPAIYLDGLDG
                     MRHCDMVRVTADGALVLTPFSSSMDELVLG"
     gene            complement(5249..>6628)
                     /locus_tag="VLI90_12385"
     CDS             complement(5249..>6628)
                     /locus_tag="VLI90_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HSV15048.1"
                     /translation="AAAKHEATTQRFIFCTGIENSYPIITNLRGQQMRRDGLQLSEHY
                     THWRHDFRLVHDLGIHFLRYGPPYYKCHAGPMQYDWSFADKTFKVLRELGIEPITDLC
                     HFGLPDWLGGSFQNADWPMLFAEYARAFAARFPWVRLYTPVNEIFICAQFSARNGWWN
                     EQLKSERAFVLALKHMCRATLLAEQAILQVRPNAQFIQSESSTYFHSQIPEARDKAGF
                     FNEWRFLALDLCYGREVNGSIYEYLNDNGLSREEYHWFMTEGPKLRRHCIVGSDYYAT
                     NEKKVVNADGAIQLSGEVLGYYQIARQYFERYRLPVFHTETNRKDDADAPRWLYKEWF
                     NILRLKADGIPVLGFTWYSLLDQTDWDVVLREDHERINPMGLYDLSRKIRAVGEAYRG
                     LIQAWHDRIPLESLSRDLSPAKPGSTLRPKSGKRRRGDVAWLSRDGKPRPIKEHPPKP
                     SRRKIEKAP"
BASE COUNT         1168 a         2062 c         2182 g         1216 t
ORIGIN      
        1 aagaagcacg tgaccgacat ccgcggcgat cggttgaaga agccgatcga tgacatcgcc
       61 ggcgggcgcg tctatacagg caagcaggcg ctggcgctgg gcttggtgga caagatcggc
      121 acgctgtccg atgcgatcgc cttcgccgcc gatcaggcga agatggcggc tgattatgac
      181 gtgcgcacgg tgccggagcc gcagaacgtc ctgcagcgca tgatggaaga cgccaacggc
      241 ggcgagaacg accccagccg ggtggaggtt ggctcttcac cgatgcgcgc cgaagcaaac
      301 ccgctgctga aggcggcgat gcccctgctg gcggggctgg acccgcagca cgtgcggatg
      361 ctgtgcgatg cgctggacca gctgcagctg gtgcagcgcg aaggcgtcat catgatgatg
      421 ccgccgcagc aaccgtgggc gcgatagacg caattgcaga acgctcaacg tgacacgatc
      481 actccggccc gtgcgtatgc ttttgaatta gcagttccgg cattactgta acgactaaag
      541 catttcgcac gggccggagt gatcgtgtca cggtgaggtt tttccatgcg ccttccattg
      601 gttttcggtt gcgcgttgtt ctcgctgatc gcaatcaccg ctcacgcggc gacggacgat
      661 cccttcacct tccccgagac ggacctgatg cgtgccgccc cgcgggcggt cttcgtcgcg
      721 ttcatgggcg actttcacaa tcccgtcggt tcgggcagat ggggcgcgtg ggctcacacc
      781 ggtgagaaaa acgccccgca cgatccgggc catatcgatc ccgccaccgg ccggcgcgat
      841 atcgcttcgt gcttctatcc gtcgatcggg ccgtacgacc agggcgatcc cgacgtcatc
      901 gattgccagt gccagctgct gaagatggcc ggcatcgacg gcatcagctt caacctcagc
      961 ttctaccaac tcgacgaatg gcggcagcgc agcatgagga tgtacgtcga tgccatgaag
     1021 cgctacgcac tgctcgcgat cgttcggttc gaaaacaagt tttttcccga caaatatccc
     1081 gacccaacgg aaatgctcgc cgcgtcgtac agcgacatgg acgcatggat gaagatgatc
     1141 gagccggtgc agtaccgggt cagtggccgg ccggtgttca tgctcttcac gttcaagctg
     1201 acgccggatc agctgcgcgc ctgggccgat agattcccgg tggctcagcg gccgatcatc
     1261 ctcacctacg gcgccgaccc caaatacaaa tccgtcgtcg atggccgatt cggctggacc
     1321 ggcgatcaac cgaatcgcct gaaagaccat ccgccgtatg tcagctatgt cacgccggaa
     1381 ctgatgcggg tgaacgaacg ccgcgaccgc cagcgcgcgc tggagctgct ggaaagcggc
     1441 cagatcacct tctatatggc cggcgtcgcg cctggcttcg acgacatcgg ctgctggggt
     1501 tggggcaacg gcccgcgcaa ggtcgagcgc gacgacggcc aaacgtatca ataccgctgg
     1561 cagcagacgc tgaagacgaa cctgcgcaac gtcttcatcc ccacgtggaa cgactggaac
     1621 gaaggcacga cgatcgagcc atcgatcgaa tacggcgatc aatatatcac catgacccgg
     1681 caatacgcgg cgcagttcaa gcagaccgcg ccgccgagca gcggcagttt gatgctgccg
     1741 atttggatct acaagatccg caagaccacg cccgacgccg cggcggatat gtcgcgcgcg
     1801 tcggagctca tcgtcaccgg cgcgttcgac caagctcgcg cgctcgtcga gccatggtgg
     1861 aaaaagctga atctggacga caagactccg tgggatcgcg ccacgcaata gccggatcgt
     1921 ctcggctcca ggcgcgcatc gcgctcgctc tcctcaagat cccatcggct ccgccgcgcg
     1981 tgccggcacc ggctccatcg cgccgccggt gtacacggtc gcgcgctgat atcgcttgcg
     2041 gaactgtgcc ggcgattcgc cgaccagcgc gccgaacgcc tcggtgaacc acgtcgatcg
     2101 cccgaacccg ctgtgccgcg cgacatcgtt caccgacaag tccgtcgtcc ccagcaggtg
     2161 cttcgcgcgc tcgacatgca cgcgccggat ctcttccagc ggcgatcgct tcagcacggc
     2221 gcgaaatcgc ttctccagcg accgccggcc gatcggcacg gcttgcaata catcatccac
     2281 gctcgtccgc tcgtgcgcgt gttcgcggat gaatcgaatc gccgccgcca cgtcggggtc
     2341 gctcacggcc acgctgtcgg tcgattgccg ctccaccacg ccgatcggcg gcagcaggat
     2401 cacgctcggc tcgatcgact cgccatcgag catgcgatcc agcgccgccg ccgcgtcgta
     2461 accgatgcgc cgccacggca ccgccacgct tgacagcggt ggatccgaaa cttcgcaatc
     2521 cagatcgtcg ttgtcgcagc ccaggatcgc cacgtcatcc ggcacgcgga tgccttcgtt
     2581 ccggcacatc tgcgcaatcg atcgcgcgcg aatgtcatta catgcgaaca gcgcgagtgg
     2641 ccgcggcgcg actcgcaaaa aatccagcag cggccccgcg cgatcgatca tgtcccaccc
     2701 gcggccggtg atggtggact ccgaatcctg ttgcagcatc gaacagccga accccgcgct
     2761 ttcgatctcg gtgcggaacc cttcgccgcg ctgatgcgac cagctggact cccggcccac
     2821 gaaggcgaat tcgcgatagc ccttctgcac gaaatgtcgc gccgccacct gccccacgcg
     2881 caagtcatcc gattcaaaat ccatcgtcac cgcgatgcca tcttccgccg gaaaaccgca
     2941 cacccccaca caccgctgca cgcaagccac cgcctgccgg ccttgttcgg cattcgacag
     3001 cgcccccagc agcagcccct gcggcttgtc ctgctcgaca aatcgcgcca ggttatccac
     3061 cggcaggtaa tgatgcagtt cccacgaagc cgtggcatta gcaaacgcgg tgatcccgcg
     3121 aagcaattcg cggtagcagg tcatgtgggt ggccatgacc actgcgatgg ttctgcgcgt
     3181 ggatgaaccc atggcagcac gagatcctaa ccgattcttg ggctcgtcgg gcgcaaagcc
     3241 cgcgatgcat ccgacacggc accggtaccc gtcgctcggt gaccgctgcc gcttgcggct
     3301 tagcatctgt tgtagttgag atacctgacg gatgctaaac cgcaagcggc ggtcgggccg
     3361 ttcttgccac cgcaacgggt tctccaggtc gttggccaaa tcgatcactt ctgcttcggc
     3421 gtcaacttat acagcgcttg ccggttggcg gtttggcctt tgaagatgag ggtggtgccg
     3481 ttggggatct tcaccaactg ctcttccccg aggttgcagg tggcgacggc gaccttatcg
     3541 aagtcggatt cggtgtaagc gaaggcgttg agggtgccgg tggcacggat ttccttcgac
     3601 tggctgctgg tgaaagccag gtccgcgtca tgctgggtgg atccgctggc gctggcgggt
     3661 cgcgtgaact tgacgtcgga cggcaggtgg tggggctggc cgtccaggaa aagcacagcg
     3721 cccggcaggc cgttgaagac gaccttcgac gtggccgtgc agccgatcgt gatgagcagc
     3781 atcgtggcga acgttccgcc catgcgattt ttctaaaggt gactgcgttg tgtcccattc
     3841 gagtctccgg tgaaagcagg catgctcgcg cgcgcgcacg ctgaagggcc cagctgccgc
     3901 tttgcggtca acatcgccgt catgagaggt gccgtccatt ggatctaagt ccaacggcat
     3961 ggcatcgcga tccgccgcga cgcgttcggc cggcattttg cttgctgctg atgcggcgat
     4021 ggactcgctt ccagaacagg ctcgaccgat ggatgccctt cgcgggcagc gggtgaccga
     4081 tgcgctgtcg gcggcggggc tggacgcggt ggtttgcgcg ctgccgacga acgtgctgct
     4141 cttgagcggg tattggccga ttgtgggtac gggcgtgatc gtcgcggggc gcgagcggcg
     4201 gattctgctg gtgccaaagg acgaacaatc gttggccgag cgcggtcatg cggacgaggt
     4261 gcggacgttt gagccgggcg gatcgctcac gcagatggcg acagcggcgg agagcgtgcg
     4321 tgatccgctg gcggacatcg cgacagagtt ggggatcgag cgcggccgca tcgggttcga
     4381 agccggcgag tggttcgagc cggcgtcgta cgcggcgatg cacctctacg ccggggcgat
     4441 gcaccgcgtg ctgcaatcgg cgctgccgag cgcgacgatc gaaccggcag acgagatgct
     4501 gcggtcgctg cgcacggtga agacgccggc cgagcttgat cgcatcagcg ccgcgtgcgc
     4561 gatttgccag accgcgttca cccgcggcgc gggtgaagtt cgagccggcg tgagcgaagt
     4621 ggaagcggcc gcgcgattcc gcgtgccgct ggcgtcgccg ctggccagcc gcgcggatgg
     4681 gcatgtcgcg ttcatgtccg gcatcaactc ggccagcgcg tatggcgcgt atgctcgatc
     4741 gaccgacaag gtgatggccc gcggcgaatt ggtgctggtc cactgcaact cgtacgccga
     4801 tggatattgg accgacgtga cgcggacgtt ctgcctcggc ccgccggatg agcggcgacg
     4861 gcggatgtac gacgcggtgt tcgccgcgcg cgacgcggcg ttcaaggcca ttcgtcccgg
     4921 cgcgcggggg gcggatgtgg atcgcgccgc gcgcgacgtg ctggccgccg cgggattcgc
     4981 cgcggcgttc aagcatccca ccgggcacgg cgtcggtttc gccgcgatcg atcataacgc
     5041 gcgcccgcgg gtgcatccga aaagcgacga cgtgctcgag gcaggaatga tcttcaacgt
     5101 cgagccggcg atttacctcg acggtctcga tggcatgcga cactgcgaca tggtgcgcgt
     5161 gactgccgat ggcgcccttg tgttgacgcc gttctcgtct tcgatggacg agctggtatt
     5221 gggttgatct gcgcgcggag cgcccggctc acggcgcctt ttcgatcttg cgccgactgg
     5281 gttttggtgg gtgctctttg atgggccgcg gcttgccgtc gcgcgacagc catgcgacat
     5341 cgccgcggcg gcgtttgccg gatttggggc gaagagttga gccgggcttg gcgggcgaga
     5401 gatcgcggct gaggctttcc agcgggatgc ggtcgtgcca tgcctggatc aacccgcgat
     5461 aggcttcacc aacggcgcgg atctttcgac tcagatcgta caggcccatc gggttgatgc
     5521 gttcgtgatc ttcgcgcagc acgacgtccc agtcggtctg gtcgagcagg ctataccagg
     5581 tgaagccgag gaccgggatg ccatcagcct tgaggcgaag gatgttgaac cattccttgt
     5641 aaagccagcg cggcgcgtcg gcgtcgtctt tgcgattcgt ctcggtgtgg aagaccggca
     5701 ggcggtagcg ctcgaaatac tggcgggcga tttggtagta acccagcacc tcgccgctca
     5761 gctggatggc gccgtcggcg ttgacgactt tcttttcgtt ggtcgcatag tagtcgctgc
     5821 cgacgatgca gtgccggcga agcttcgggc cttcggtcat gaaccagtgg tattcctcgc
     5881 gcgacaggcc gttgtcgttc aggtattcgt agatgctgcc gttgacctcg cggccgtagc
     5941 acaggtcgag cgcgaggaag cgccattcgt tgaagaagcc ggccttgtcg cgcgcctcgg
     6001 gaatctgcga atggaagtag gtgctggatt cgctctggat gaactgtgca ttggggcgga
     6061 cctgcaggat ggcctgctcg gccagcagcg tggcgcggca catgtgcttc agcgccagga
     6121 cgaaagcgcg ttcggatttg agctgctcgt tccaccagcc gtttcgcgcg gagaactgcg
     6181 cgcagatgaa gatctcgttc accggcgtgt agaggcgcac ccaaggaaag cgcgcggcga
     6241 acgcgcgggc gtattcggcg aagagcatcg gccaatcggc gttctgaaag ctgccgccca
     6301 gccagtcggg caggccgaag tggcataggt cggtgatcgg ttcgatgccg agttcgcgca
     6361 gcaccttgaa cgttttgtca gcgaaggacc agtcgtactg catcggcccg gcgtggcact
     6421 tgtagtaggg cgggccgtag cgaaggaagt ggatgcccaa gtcgtggacg agccgaaagt
     6481 catgccgcca gtgcgtgtaa tgctccgaca gctgcaggcc atcgcggcgc atctgctggc
     6541 cgcgcaggtt ggtgatgatc ggatagctgt tttcgatgcc ggtgcaaaag atgaagcgtt
     6601 gggtggtggc ttcatgctta gcggcggc
//
LOCUS       DATIMH010000706         8285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5656100, whole genome shotgun sequence.
ACCESSION   DATIMH010000706 DATIMH010000000
VERSION     DATIMH010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5656100"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VLI90_12390"
     CDS             <1..1307
                     /locus_tag="VLI90_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSV15049.1"
                     /translation="LPKEACCECLTGLIAAQCKGDHRQARNFREFMDNVFGDGIVKHF
                     MKPYNFKVWAHPPKMMNKEWIGERVAVLDIQRALKNVVLGTDDFGWGPNNQFKFPLFG
                     GTGEFYRRFGKPLDGHVHLNKTVDFINLVKKEIRFKDGEIVKYDKLISAMPLDILCND
                     VLNGEVPREIKKSTASLRHSGGYMVGIGIKQPCPSTKSWMYFPEDNCPFYRVTYLSNY
                     SPYMTPDAKTHYSLLCETSYSEFKPVDGRTIVEDTIKGLINAGMLKEEDRETIVDTWT
                     YHAKYSYPTPSVERDKILSEAIPFLEKHDIYSRGRFGMWKYEVSNTDHTLMQGVELVN
                     KWYLGEAESTIGIKYESTLDGRNAAVHERSVLAGSGDPKRTPRENPDAAKAAAHPGEK
                     AAPHFNVTKPKTGIGGPGDKVEVHTSEEELGVTTPLEQEDEK"
     gene            complement(1707..2291)
                     /locus_tag="VLI90_12395"
     CDS             complement(1707..2291)
                     /locus_tag="VLI90_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV15050.1"
                     /translation="MIVEDDTIVGMGLKAQLERIGHRVVGQAANAAEGQRLFREHKPD
                     LLLVDIHLNGDDGIDLLRQLLAERRVPAIIITAYSDRELIERATAAGVFGYLIKPISP
                     QALDAQITVAVQRFAEQEKLQQEKQALTQTLEERKLVERAKGIFMRRLNLDEPEAHKR
                     LQQESQKRRVPMVELAKRIIESEEMLGSGGGSAS"
     gene            complement(2327..5056)
                     /locus_tag="VLI90_12400"
     CDS             complement(2327..5056)
                     /locus_tag="VLI90_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSV15051.1"
                     /translation="MHGRPAARDPHGPHGLARGGLRYRLSQAMHLGGASVRATGAVPL
                     ALLVLLLGLTFALWQYQRTRVVDDLRDDAQMIRTQGARRLERAAEARIHLLRVLATEA
                     GEFPPALFGTAASSTCDVISGCQGIARCDAAGLAISNFAGSSAASKAPADITAVADWR
                     ALWPRLLARQAPVVSRVYGVAGASGYVLVLAPIIDNGKFDGAIVCRMRLSDLMDRALD
                     RSIRRDYDIRVSDGVNTAFTFDGAENWQPDPADGEPIVFPGTTWTIYSRPNQALLDSA
                     LRARPTPILLIGVAAAFAVAAAVWQANRYRMRDLAQARAHSNAVERLHSVAAAISAHA
                     GSGQDVLQRLAKAACELLQMTRANILVLETPSRGNSVETRWRSVGNLDGAELRVVANA
                     GWPESFLGHRYALASLPASQLAIREARVLLIRDVATSDLPVNLFREHGLRSGILIPLQ
                     IEGRVTGLLALSSEQAREFTSDDQRLAELWGAQAAVTLANAQLHQQARDALAVQRQLL
                     EQREALATATAAIYAEPHLEGTLREIAHRTPAVLGVDFCGVTLITPDNEELVIAAATP
                     PHDHVVGWRIKRPYAPVEAMFTARQPRIVEDITKEPQLDSVWQRIRSAGSFFNVPLYS
                     SNQQPLGVLTLVRYAKGSFRPDQLELAQLFAARAATAIENNRLHEQAHRDAETKAMLL
                     RELNHRVKNNLARIVGLLAMGENNVPELAKPTLARLAQRVRMMSGAHDLFAGGVDMIS
                     LEDLVQRVLASLSLARPIGVDVRLELCNVGVWLNTDRAVSLAMVLHELAFNAVTHGTI
                     ERGLMEIRAHVTDDARLRIEVLDDGVGIDPRCCDESGTSSGNGAANQLDNDADAAVAV
                     AGSSRTGLGLPLVRGLVQRELGGAFEIRPRFAGGTCAGVEFPLTARERQKDVL"
     gene            complement(5235..5753)
                     /locus_tag="VLI90_12405"
     CDS             complement(5235..5753)
                     /locus_tag="VLI90_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HSV15052.1"
                     /translation="MSESLRLLTDDSLLGQCRWDVFRGSGPGGQKRNKTSNAVRLVHE
                     RTGIAVTATESRSLAENKLWAVRRMRIKLAADLREPIDLARFEAPDWFCAIRNNRRIE
                     ASHRHPLYAATAGLMLDLLAALAGNPAAVAVNLGVSTTAVIRLLQNETPWWATANRIR
                     NEFGLPVLSQHR"
     gene            5902..6861
                     /locus_tag="VLI90_12410"
     CDS             5902..6861
                     /locus_tag="VLI90_12410"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV15053.1"
                     /translation="MSQHSLSNRGPRAVVGRHEGAQLIEPLEPRTLFTVLLFQPATGA
                     FADYQPLPQTYGDRVTAATQNGFKYGTAGGTTPKVLASYGTSTGTIETWPTAYGDLPA
                     VAFAAPSGQKFEMKLTADSGFNVTLASFDMASFASADYTINSVSVVDSSGKTLFSKSN
                     VKIFGGTAPHHTHFAFTTPLKGQGLTIRFDPSNQGGWNVGIDNVQFSQVALQTTTITG
                     NVFADTNANGKKDTGEAALSGWRVYIDTNNNGMYDSGEQFVLSDSTGKYSFTFSTPGT
                     YIVSVQLKRGYYQTSPHALIYTVKAPSANVTNALFGVKTIAPV"
     gene            complement(7058..>8285)
                     /locus_tag="VLI90_12415"
     CDS             complement(7058..>8285)
                     /locus_tag="VLI90_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HSV15054.1"
                     /translation="LNGGDGADYMSGGAGTDTVDYSGRNEALSIWMSLDGGNVSGSSA
                     HDIRWTGDHYTGDRIEGDIENCNGGSGNDVIYGNSLNNLINGNGGNDQLYGLAGNDSL
                     NGGAGEDFLSGGDGNDQLHGGVGDDTMRGGAGADNIFGDAGFDFADYQDHTSGLTAVS
                     LNGVADDGTFTLFTSEADNVQMDVEGIIGAVSGNNLLQGNDGPNELIGGSGNDTIYGN
                     GGNDSIYGGAGADQLHGGAGDDSIYGDIGNDIMWGDDGNDTLRGGQGADVFWGGAGAF
                     DEADFADRTENLNLTLDDAANDGAAGEGDNIHSDVEIIIGGFGNDILTGDAADNQLYG
                     MAGNDTCEGMGGDDYLLGGDGNDYLHGGSGHDTLAGQAGNDIFYSLDGEPDILYGNSA
                     QDSFFMDGFDTFIAST"
BASE COUNT         1574 a         2849 c         2413 g         1449 t
ORIGIN      
        1 atctgcccaa ggaggcatgc tgtgagtgcc tgaccgggct aattgccgcc caatgcaagg
       61 gcgatcatcg ccaggcgcgg aacttccgcg agttcatgga caacgtcttc ggcgacggca
      121 tcgtgaaaca tttcatgaag ccgtacaact tcaaggtctg ggcccatccg cctaagatga
      181 tgaacaagga atggatcggc gagcgcgtcg ccgtcctcga catccagcgg gcgctcaaga
      241 acgtcgtgct cggcaccgac gatttcggct gggggccgaa caaccagttc aagttcccgc
      301 tgttcggcgg caccggtgag ttctaccgcc gcttcggtaa gccgctcgat gggcacgttc
      361 acctgaacaa gacggtggac ttcatcaacc tggtgaagaa ggaaatccgc ttcaaggacg
      421 gcgagatcgt caagtatgac aagctgatct ccgcaatgcc gctggacatc ctctgcaacg
      481 acgtcctgaa cggtgaagtg ccgcgcgaga tcaagaagtc caccgccagc ctgcggcaca
      541 gcggtgggta catggttggc attggcatca agcagccgtg cccgtcgacc aagtcgtgga
      601 tgtatttccc cgaagacaac tgcccgttct atcgcgtcac gtatctgagc aactacagcc
      661 cgtacatgac gccggacgcg aagacgcatt acagcctgct gtgcgagacc agctacagcg
      721 agttcaaacc ggtcgatggc cgcacgattg tggaagatac gatcaagggc ttgatcaacg
      781 ccgggatgct gaaggaagaa gaccgcgaga caatcgtcga tacgtggacc tatcacgcga
      841 agtacagcta tccgacgccg agcgtggaac gcgacaagat cctgagcgag gcgatcccgt
      901 tcctggagaa gcacgacatc tacagccgcg gccgattcgg catgtggaag tacgaagtct
      961 ccaacaccga ccacacgctg atgcagggcg tggagctggt gaacaagtgg tacctcggcg
     1021 aagccgaatc gacgatcggc atcaagtatg agagcacgct cgacggccgc aacgccgcgg
     1081 tgcacgagcg ctccgtgctg gccggcagcg gtgacccgaa acgcacgccg agggagaatc
     1141 ctgacgcggc aaaggctgcg gcgcatccgg gcgaaaaggc ggcgccgcac ttcaacgtga
     1201 ccaagcccaa gaccggcatc ggcggtcccg gcgacaaggt agaggttcac acgtccgagg
     1261 aagaactcgg cgtgacgacg ccactggaac aggaggacga gaaatgaccc gcaacacccc
     1321 gaataaacac ccgggccagg aagcccgccc caacccgcac cgctggggta agaaaaccaa
     1381 ccccgaccgc gtgcgacccc tgcgggcgca ccggccgtga gttagatcga tccattgctt
     1441 cccctagcga acgggccacc caccacgggt ggcccgttcg cgttttgccg ccttgtcctg
     1501 agcgcaggcg gtactccgcc ggagtcgaag gatcttgccc agaagtcggt gaccgatgac
     1561 tcgcgctatc cgcgcagcag tcgcttgcgg tttcgcaaac cagatcgcct ggattgctac
     1621 gcgaagacgc gaagcatcgc gcgagtctca agctccaaat acttctcagc cagatccttc
     1681 aactgcgcat gatcaagtgc gcccgctcag gatgcacttc cacccccgct ccccaacatc
     1741 tcctccgact cgataatccg tttcgccaac tccaccatcg gtacccgccg cttctggctt
     1801 tcctgctgca atcgcttatg agcctccggc tcatccaggt tcaaccgccg catgaagatt
     1861 cccttcgccc gctcgacaag ctttcgctcc tccagcgtct gcgtcagcgc ctgcttctct
     1921 tgctgcagtt tctcctgctc ggcaaaacgc tgcaccgcca ccgtgatctg cgcatccagc
     1981 gcttgaggac tgatcggctt gatcaggtaa ccaaacacgc cggccgccgt cgcgcgctcg
     2041 atcagctcgc gatcgctgta cgccgtgatg atgatcgccg gcacccgccg ctccgccagc
     2101 aactgcctta gcagatcaat gccgtcatca ccattgaggt ggatgtcaac cagcaacaaa
     2161 tccggcttat gctcgcgaaa caaccgctgg ccctcggcgg cattcgctgc ctgccccacc
     2221 acgcgatgac cgatgcgttc cagctgggcc ttcaggccca tgcccacgat cgtgtcatct
     2281 tcgacgatca atacctgcct tccccctgaa attccgccac tctgactcat aacacatctt
     2341 tctgccgctc tcgggcagtc aggggaaact cgacgccggc gcacgtgccg ccggcaaaac
     2401 ggggacgaat ctcgaatgca cctcccaact cgcgctgcac caggccgcgc accaggggca
     2461 ggcccagacc cgtccgcgat gaaccggcca cggccaccgc ggcatctgcg tcgttgtcca
     2521 actgatttgc ggccccattg ccgcttgatg tgccgctctc atcgcagcag cgcggatcga
     2581 tgccgactcc gtcgtcgagc acctcgattc gcaggcgagc gtcgtcagtg acatgggcgc
     2641 ggatctccat caatccgcgc tcgatcgtgc cgtgcgtgac ggcattgaac gccagctcgt
     2701 gcaggaccat cgccaggctg acggcgcggt ccgtgttgag ccaaacacct acattgcaaa
     2761 gttcgagccg tacgtcaaca ccgatcggcc tggccagcga cagcgacgcc agcacgcgct
     2821 gcaccaggtc ttccaggctg atcatgtcca ccccgccggc gaataagtca tgcgcccccg
     2881 acatcatgcg cacccgctgc gccaatcgcg ccagcgttgg cttggccaac tccggcacgt
     2941 tgttctcccc catcgccagc aatccgacga tccgcgccag gttgttcttc acccggtggt
     3001 tcagctcgcg cagcagcatc gccttggtct cggcgtcgcg atgggcctgc tcgtgcagcc
     3061 gattgttctc aatcgccgtc gccgcccgcg ctgcgaataa ctgcgcgagt tccaactggt
     3121 ccgggcgaaa tgaccccttt gcatatcgca ccaacgtcaa cacgcccagc ggttgctggt
     3181 tgctgctgta cagcggcacg ttgaagaagc tgcccgcgct acgaatgcgc tgccagacgg
     3241 aatccagctg cggttccttg gtaatatcct cgacgatgcg cggctgccgc gcggtgaaca
     3301 tcgcttctac cggtgcatag ggccgcttaa tgcgccagcc gacgacgtga tcgtgcggcg
     3361 gcgttgccgc ggcgatcacc agttcctcgt tgtccggcgt gatcagcgtc acgccgcaaa
     3421 aatccacccc cagcaccgcc ggggtgcgat gggcgatttc gcgcagcgtg ccttccaggt
     3481 gcggctcggc gtaaatcgcc gcggtcgccg tcgccagcgc ctcgcgttgc tccagcaact
     3541 gccgctgcac cgccagcgcg tcgcgcgcct gctgatgcag ttgcgcgttc gccagcgtca
     3601 ccgccgcctg cgcgccccac agctccgcca ggcgctggtc atccgacgtg aactcccgcg
     3661 cctgctcgct cgatagcgcc aacaggccgg tcacgcggcc ttcgatctgc agcgggatca
     3721 ggatgcccga gcgcagccca tgttcgcgaa acaggttgac cggcagatcg gaagtggcca
     3781 cgtcgcggat cagcagcacg cgcgcttcgc ggatcgccag ttggctcgcc ggcagcgacg
     3841 ccagggcata gcggtggccc aggaaagatt ccggccaacc ggcgttggcg acgacgcgaa
     3901 gctcggcgcc gtcaaggtta ccgactgacc gccaacgtgt ctcgacgcta ttgccgcgcg
     3961 acggcgtctc cagcaccagg atgttcgccc gcgtcatttg cagcagctcg cacgccgcct
     4021 tcgccagccg ctgcagcaca tcttgtcccg acccggcgtg cgcgctgatc gccgccgcca
     4081 ccgaatgcaa ccgctcgacc gcgttcgaat gcgcccgtgc ctgcgcgagg tcgcgcatgc
     4141 gatatcggtt cgcctgccac accgccgccg ccaccgcgaa tgccgcggca accccgatca
     4201 gcaggatcgg cgtgggccgt gctcgcaggg cggaatccaa cagcgcttga ttgggacgcg
     4261 agtaaatcgt ccacgtcgtc ccggggaaga cgatcggctc gccgtccgcc ggatcgggct
     4321 gccaattctc ggcgccgtcg aacgtaaatg cggtgttgac gccatcactc acgcgaatgt
     4381 cgtagtcccg acgaatcgac cgatccagcg cgcgatccat cagatcgctc agccgcattc
     4441 ggcagacgat cgcgccgtcg aacttgccgt tgtcgatgat cggcgccagc accagcacat
     4501 aaccggacgc ccccgcaacg ccgtacaccc ggctgaccac cggcgcctgc cgtgccagca
     4561 acctgggcca caacgctcgc caatcggcga ccgccgttat gtcggccggc gctttgctcg
     4621 cagcgctgga tccagcaaag ttcgagatcg caagcccagc cgcgtcacag cgggcgatgc
     4681 cctggcaacc ggagatgaca tcacacgtgg atgacgcggc ggtgccaaac agcgccggtg
     4741 gaaattcacc ggcttcggta gccaagacgc gcaacagatg aatgcgcgcc tccgccgcgc
     4801 gctccagtcg gcgcgcgccc tgcgtgcgga tcatttgggc gtcgtcgcgc aggtcgtcaa
     4861 caacccgcgt ccgctgatac tgccaaagcg caaatgtgag ccccagcagc aagaccagca
     4921 gcgccagcgg caccgcgccg gttgcgcgca cgctcgcgcc gcccaggtgc atcgcctggc
     4981 ttagtcgata tcgcagtcca ccgcgcgcaa ggccatgcgg cccatgcggg tcccgcgcgg
     5041 ctggtcgccc gtgcacaccg cctccagaca ccccgctcgc tacaccccgg ctcccatgaa
     5101 ccgatccaat ccatacgagt ttttcaccga gaagatatgc gacaaactcg cctgcggcaa
     5161 aaggttcttg atgattcgca acggttcgca attccgtctg ctgtcgagtt ttgcagttaa
     5221 cgagttaacc cgcgtcaacg gtgttggctc aggaccggca gaccaaattc atttcgaatt
     5281 cgattggccg tcgcccacca cggcgtttcg ttctgaagca gccggatgac tgcggtcgtc
     5341 gagacgccga gattgaccgc caccgccgcg gggttccccg ccagggcggc gagcagatcc
     5401 aacatgagcc ccgccgtcgc cgcgtaaagc gggtggcggt gcgacgcttc aatgcgacgg
     5461 ttattgcgaa tggcgcaaaa ccaatcaggc gcttcgaaac gggccagatc gatcggctcg
     5521 cgcaagtccg ccgccagctt gatgcgcatc cgccgcaccg cccacagctt gttttctgcc
     5581 agcgaccgcg attccgtcgc cgtgaccgca atgccggtgc gttcgtgtac cagccgcact
     5641 gcgttgctcg ttttatttcg cttctgccca cccggccccg agccgcggaa gacgtcccag
     5701 cggcactggc cgagcagcga gtcgtcggtc agcagtcgca gcgattcgct caatgacgtc
     5761 tccagttgct gagcccgctc gatgtttggc ccgtctccac tttgtcatta tttgcttgcc
     5821 acgacggcct cagcctcggc atactccggc ccagtgggta aagggggcca aggcacttct
     5881 cagcgaaagc aaaggagaat catgtcgcaa cattcattgt caaatcgtgg accgcgcgca
     5941 gtcgtcggcc ggcacgaagg ggcgcaattg atcgaaccgc tcgaaccgcg aacgctcttc
     6001 accgtgctgc tcttccagcc ggccaccgga gcgttcgcgg attaccaacc gctgccgcag
     6061 acgtacggcg accgcgtgac ggccgccacg cagaacggtt tcaagtacgg caccgccggg
     6121 ggaaccacac ccaaggtgtt ggccagttac ggcacctcga ccgggacgat cgaaacctgg
     6181 cccacggcct atggcgatct gcccgccgtc gccttcgccg cgcccagcgg acaaaagttc
     6241 gagatgaagc tgaccgccga ctcaggcttc aacgtcacgc tcgcctcgtt cgacatggcc
     6301 agcttcgcca gcgccgacta caccattaac tccgtgtcgg tcgtcgatag cagcggcaag
     6361 acgctgttca gcaagagcaa cgtgaagatc ttcggcggca ccgcgccgca tcacacgcac
     6421 ttcgcgttca ccacgccgct gaaggggcaa ggcttgacga ttcgattcga tccgtccaac
     6481 cagggcgggt ggaacgtcgg catcgacaac gtgcagttca gccaggtcgc gctgcagacg
     6541 accaccatca ccggcaacgt cttcgcggac accaacgcca acggcaaaaa ggacacaggc
     6601 gaagccgccc tctccggctg gcgcgtgtac atcgacacga acaacaacgg gatgtacgac
     6661 tccggcgagc agttcgtgct gagcgacagc acgggcaagt actccttcac gttcagcacg
     6721 cccggcacct atatcgtcag cgtgcagctg aagcgaggct actaccagac ctcgccgcac
     6781 gcgctgatct acacggtgaa ggcgccgagc gcgaatgtca ccaacgccct gttcggcgta
     6841 aagacgatcg cgccggtgtg aacggcgcgg gttgcacaat cccccccgac gccggtgtct
     6901 gagcgtactc gcgaagaccc gggttcttgg cgcggacata accgggtctt cggagtacct
     6961 cagacatcgg cgtcgggggc cgtgcagcgc gcatcaccgg ccccgacgca agcgaagcaa
     7021 cccggttcac gaaccacctt gcttcgcttg ccacacatca cgtgctcgcg atgaacgtgt
     7081 cgaagccgtc catgaagaat gaatcctgcg cgctgttgcc atacaagatg tcgggttcgc
     7141 catccaagga atagaagatg tcgttccccg cctggccggc aagcgtatcg tggcccgagc
     7201 cgccgtgcag gtagtcgttt ccgtcgccgc cgagcagata atcgtcaccg cccatgcctt
     7261 cgcaggtgtc gttgccggcc atgccgtaca gctgattgtc ggccgcgtcg ccggtcagga
     7321 tatcgttgcc gaacccgccg atgatgattt cgacgtcgct gtggatgttg tcgccttcac
     7381 cggccgcccc gtcgttggcg gcatcgtcca gcgtcaggtt tagattctcc gtgcgatcgg
     7441 cgaagtcggc ttcgtcgaac gcgccggcgc cgccccagaa tacgtcggcc ccctgcccac
     7501 cgcggagcgt gtcgttgccg tcatcgcccc acatgatgtc gttgccgata tcgccataga
     7561 tcgaatcgtc gccggcgccg ccgtgcagtt ggtcggcgcc cgcgccgcca tagatgctgt
     7621 cgttcccgcc gttgccgtag atcgtgtcat tgccggaccc gccgatcagc tcattgggcc
     7681 catcgttgcc ctgcagcagg ttgtttccgc tgacggcgcc gatgatgcct tcgacatcca
     7741 tctgcacgtt atccgcctcg ctggtgaaga gcgtgaacgt gccgtcgtcg gccacgccgt
     7801 tcaagctgac cgccgtcaag ccgctggtgt ggtcctgata gtcggcgaag tcgaaaccgg
     7861 cgtcaccgaa aatattgtcc gcgccggcgc cgccgcgcat ggtgtcgtca cccacgccgc
     7921 catgcaactg atcgttgcca tcgccaccgg agaggaagtc ttcgcccgcc ccgccgttca
     7981 agctgtcgtt gccggccagg ccgtacagct gatcgttgcc accgttgccg ttgatgaggt
     8041 tgttcagcga gttgccgtag atcacatcgt tcccgctgcc gccgttgcag ttctcgatat
     8101 cgccttcgat gcgatcgccg gtgtaatgat cgccggtcca acgaatgtcg tgcgcgctgg
     8161 agcccgagac gttcccgcca tcgagcgaca tccagatgct gagtgcctca ttgcgaccgg
     8221 aataatctac cgtgtcggtg ccggcgccgc cgctcatgta gtccgcgccg tcgccgccgt
     8281 tgaga
//
LOCUS       DATIMH010000707         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2766788, whole genome shotgun sequence.
ACCESSION   DATIMH010000707 DATIMH010000000
VERSION     DATIMH010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2766788"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VLI90_12420"
     CDS             complement(<1..1085)
                     /locus_tag="VLI90_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSV15055.1"
                     /translation="MLIPIADVPLWYAERKPKNAVAVSHGTDELTWEQLERNANRRAR
                     AFAAKGVKPGDFVAIGLPNSNAFFETTFAVWKCGATPTSLTWRLPRGEAAAVLDILKP
                     SLVVGGQPDWNAPNSLPADFEPQGFSDEPLTNPVARYWKAMTSGGSTGRPKVILDHMP
                     AVHDTAVASALGIPQNGSLLNPGPLYHNAPFIATHVALFSGSRVTGLVKFDAEETLRL
                     IEAHKVQWVNFVPTMMHRIWALPDAVRNGYDLSSLQIVFHMAAPMPPWLKEKWIEWLG
                     PERIYELYGGTERQGATVITGVEWLAHRGSVGKIGDTSRLKIVGEDGNEVATGETGEI
                     YFLPNDGAGSTYHYLGAEPKRRPDGWES"
     gene            1546..>2645
                     /locus_tag="VLI90_12425"
     CDS             1546..>2645
                     /locus_tag="VLI90_12425"
                     /inference="COORDINATES: protein motif:HMM:NF015836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15056.1"
                     /translation="MTIASADVLPDPEAKVRTSAWRLHPLFAVILVAIAVRLPIAFWP
                     NINHPDEIFQYLEPAWRMLGHDTIVTWEWRDGIRGWFLPTLFVGPVALGDWLAPGGRG
                     AFMVSRLVVAFASLSIVISAWCFGARISRTHAIVAALATAVWFELVYFAPHTLGEPLA
                     TALILPAALLLTGGLSQKRLLIGGGLLALAFVWRFQYAPTMVIFVLGACWRQWRNAIP
                     LIVGGLVVLSLAAMVDVAHGVVPFEWLFRNIEQNLLHDRASEFGVAPAIAYVQLLLVI
                     WSGAVVLLLCALWRGWRHAPLLIVAAVVNIAFHSSIEHKEYRFIFLSVALLIIAAALG
                     SGDWVQRMQEKPAWRPWALPVVAGGWVLISLG"
BASE COUNT          445 a          777 c          858 g          565 t
ORIGIN      
        1 gattcccagc cgtcaggccg gcgcttcggt tcggcgccga gatagtggta ggtgctgccg
       61 gcgccgtcat tgggcaggaa atagatctcg ccggtctcgc ccgtggcgac ctcgttgccg
      121 tcctcgccga cgatcttcag gcggctggtg tcgccgatct tgccgaccga gccccggtgc
      181 gccagccatt cgaccccggt aatgaccgtg gcgccctgcc gttcggtgcc gccatagagt
      241 tcgtagatcc gctccggccc cagccactcg atccattttt ccttcagcca cggcggcatc
      301 ggtgcggcca tgtgaaacac gatctgcagg ctggagagat cgtagccgtt gcgcacggcg
      361 tccggcagcg cccagatccg atgcatcatg gtcggcacga aattgaccca ctgcaccttg
      421 tgggcctcga tcaggcgcaa cgtttcctcg gcgtcgaatt tgaccagacc ggtgacgcgg
      481 ctgccggaga acaacgcgac gtgggtcgcg atgaacggcg cgttgtgata gagggggccg
      541 ggattgagca gcgagccgtt ttggggaatg ccgagcgccg aggcgaccgc cgtgtcgtgc
      601 accgcgggca tatgatcgag gatcaccttg ggccggccgg tcgagccacc gctggtcatc
      661 gccttccagt agcgcgcgac gggattggtc agcggttcgt cagagaaacc ttgcggctcg
      721 aaatccgccg gcagcgaatt cggcgcgttc cagtccggtt ggccgccgac caccagcgat
      781 ggtttgagaa tatcgagtac ggccgcggcc tcgccgcgcg gcagccgcca ggtcagcgac
      841 gtcggcgtcg cgccgcactt ccacactgca aacgtcgtct cgaaaaatgc gttgctgttc
      901 ggcagtccga tcgcaacgaa atcgcccggc ttgacgcctt tggcggcaaa ggcgcgagcg
      961 cgacggttgg cgttgcgctc cagctgctcc caggtcagtt catcggtgcc gtgactgacc
     1021 gcgacggcat tcttgggttt gcgttcggca taccacaggg gcacgtcggc gatagggatc
     1081 agcatggggc gtttccgtgg tggggcggcg tgcttggccg ctcttgtgct tcgtctccgg
     1141 cggttcgccg caagtcatac acttcaccaa cggaagctaa ggtgagtcaa gacgccgggc
     1201 tacggaaacg ctggggtgtg cggaagcatt tccctaccgg ccatggaaca acagtcagac
     1261 gaagaattcc tgtcggtgcg cactcggcaa tccgattcgg cgctcgcttc gtgcgataat
     1321 acagaaaaaa aggaacgcaa atgccagaaa ccaacccgtg gacgcgattc cggcagaccg
     1381 gctgttcagg agcctaaacc cgttgtctaa aatccgatgc accgacgata tgacagtgac
     1441 ttgcggtggt tggtcggtca cgcatcctta aaggttcgaa ggtatgagtt cagctgaaaa
     1501 gtcgctccag cggggcgaac cttctgaatt gcagtcggcc ggagaatgac gatagcgagc
     1561 gctgacgttt tgcccgaccc ggaagccaaa gtcagaacct ccgcctggcg gcttcatccg
     1621 ctgttcgcag tgattctggt agctatcgca gtccggctgc cgatcgcgtt ttggccaaat
     1681 atcaaccatc ccgacgagat atttcagtac ctggagccgg cctggcgcat gcttggccat
     1741 gatacgatcg tcacctggga gtggcgtgac ggcattcgtg gctggttctt gcccaccttg
     1801 ttcgtagggc cggtcgcatt gggagactgg ctcgcgcccg gaggcagggg cgctttcatg
     1861 gtctcccgct tggtcgtcgc gttcgcgtcc ctgtcgatcg tgatcagtgc gtggtgtttc
     1921 ggtgcacgga tatcacgaac gcatgcgatc gttgcagccc tggccaccgc ggtctggttt
     1981 gaactcgtct acttcgcgcc gcatacgttg ggcgaaccct tggcgaccgc acttatcctg
     2041 ccggccgctt tgttgttgac aggcggcctc tcgcagaaac gccttctcat tggtggcggg
     2101 ctgctcgcgc tcgcatttgt ttggcgcttt cagtatgcgc cgaccatggt catattcgtg
     2161 cttggggcat gctggaggca gtggcgaaac gcgatcccat tgatcgttgg agggctcgtc
     2221 gtcctgtcgt tggcggccat ggtcgatgtc gcacacggcg tggtgccgtt tgaatggctg
     2281 ttccggaata tcgaacagaa cctgctgcat gaccgggcat ccgaattcgg agtggcgccg
     2341 gcgatcgcct acgtccagtt gctgttggtg atctggtcgg gagcggtagt gctgctgcta
     2401 tgcgcgctct ggcgcggttg gcgacatgct ccgctgctga tcgtcgcggc agtcgtgaat
     2461 atcgcctttc acagctcgat cgagcacaag gaataccggt tcatattttt gtcggttgcc
     2521 ttgcttatca tcgcggcggc gcttggctca ggcgattggg tgcagaggat gcaggagaag
     2581 cccgcgtggc gtccctgggc gctgcctgtg gtcgccggag gttgggtgtt gatctctctg
     2641 ggaca
//
LOCUS       DATIMH010000708         3813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4793407, whole genome shotgun sequence.
ACCESSION   DATIMH010000708 DATIMH010000000
VERSION     DATIMH010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4793407"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="ilvC"
                     /locus_tag="VLI90_12430"
     CDS             complement(<1..838)
                     /gene="ilvC"
                     /locus_tag="VLI90_12430"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:TIGR00465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HSV15057.1"
                     /translation="MERIYRDADVDLAVLKGKTIAVIGYGIQGKAQAANARDSGCHVI
                     VGTRPTEESKSRDHAKADGFDAMSIADATKKADLLLIELADPVQPSIYKKDIAPNLRA
                     GQTLCFCHGFSVLYGQIQVPKDVNCVLYVPNAPGAFVRAKYQKGEGVYGCVSVDQDAT
                     GNARQIVLAISKAVGSTRAGVVEMTFQHETEGDNFEEQILYGGAIQLMRLCFQTMVDN
                     GYPPSFAYAKAIRSLRSVIDVMDENGIEDYLTGRCSRTCEFAVRTRGPRVINENEIRK
                     IFA"
     gene            complement(1030..1764)
                     /gene="allE"
                     /locus_tag="VLI90_12435"
     CDS             complement(1030..1764)
                     /gene="allE"
                     /locus_tag="VLI90_12435"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HSV15058.1"
                     /translation="MHSRARVSRRYALFPREGYPTSRLPAWDKTEAFVLAAPALGAQF
                     VQYLLKVEANGGTQHEADGRVETFFCLLSGEALLSASDGQSWKLTPGSFALMPPTLGY
                     ELRMRQAGELLTLRKVYEPADGVEMYQPLVGNESNVPGPPWINNEHARLQTLIPDELQ
                     YDLAMNIFTFDPGHGLPYVETHVMEHGLYFLQGKGVYYLEDTWMEVESNDFIWMGPYC
                     PQSFYATGPTPAKYIYYKNVNREIAL"
     gene            complement(1825..3525)
                     /gene="ade"
                     /locus_tag="VLI90_12440"
     CDS             complement(1825..3525)
                     /gene="ade"
                     /locus_tag="VLI90_12440"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HSV15059.1"
                     /translation="MSLSDLLAVARGDAPADLLLRNARIVNVFTGEIEPATDIAIAHG
                     HIAGIGPDYTAKESIDLANALIAPGLIDAHVHIESSLCVPAHFAGAAMPRGVTTVVTD
                     PHEIANVAGVAGIRFMADSRRGVPLRIEIMAPSCVPATDMGTCGAQLTSNDLAPLLRD
                     GTVRGLAEVMNFPGVMLGDPHVLAKLAAFQRTPIDGHCPGVRGSPLNAYIAAGIGSDH
                     ESVAVDEAKEKLARGLYVFIREATNARNLRALLPLITPANSRRICLCTDDRQAADLLG
                     DGGVDYMIREAIAFGIDPVTAFRLATLNTAEYFGLHDRGAIAPGRVADLMVFDDLRRP
                     VAREVFIAGQSIRDYRPTPLAASWAISNTCRVNWDRVDLRIEARGERVRVIGSLPDQL
                     VTEHRVQPASIVSGFAIADASRDLSKMAVIERHRGTGNIGLGFIQGFGLKTGAIAGTV
                     AHDHHNLVVIGADDISMMTAARAAAGEGQGGLAVAEGQQVLAMLPLPVAGLMSDRPIE
                     EVRSAYNALLSAAAKLGSSLHDPFMAMSFMALEVIPALKLTDQGLVDVETFSKVDLFV
                     "
BASE COUNT          654 a         1161 c         1232 g          766 t
ORIGIN      
        1 cggcgaagat cttgcggatc tcgttttcgt tgatcacccg tgggccgcgc gtgcgcacgg
       61 cgaattcgca cgtgcggctg catcggccgg tgagataatc ttcgatgccg ttctcgtcca
      121 tcacgtcgat cacgctgcgc aagctgcgga tcgccttggc gtacgcgaag ctgggcggat
      181 agccattgtc caccattgtc tggaagcaga ggcgcatgag ctggatcgcg ccgccgtaga
      241 ggatctgctc ttcgaaattg tcgccttcgg tttcgtgctg gaaggtcatt tcgacgacgc
      301 cggcgcgggt ggagccgacg gctttggaga tggcgaggac gatctgcctg gcgttgccgg
      361 tggcgtcctg gtcgacgctg acgcagccgt acacgccttc gcctttttga tatttcgcgc
      421 ggacgaatgc gccgggcgcg ttcggcacgt acagcacgca gttcacgtcc ttcggcacct
      481 ggatctgtcc atacagaacg ctgaagccgt ggcagaagca aagcgtttgc ccggcgcgaa
      541 ggttgggggc gatgtccttc ttgtagatgc tcggctgcac cgggtcggcc agctcgatca
      601 gcagcaggtc ggccttcttg gtggcgtcgg cgatgctcat cgcgtcaaaa ccatcggcct
      661 tcgcgtggtc gcggctcttc gattcctccg tcggccgggt gccgacgatg acgtgacacc
      721 cgctgtcgcg cgcgttggcc gcttgcgctt tgccttggat gccatagccg atgacggcga
      781 tcgtcttgcc tttgaggacc gccagatcga catcggcgtc gcgataaatc ctttccactt
      841 cttactccct gaaatacaca tgcgaccgcg aagacgcgaa gacagacgcg aaacaagcga
      901 gaaaggcaga agccgcgaat ggcacgaatg gaatcgatca atggctctga ttcgtgctca
      961 ttcgtgtcat tcgtggcttg ctcttctttt tgttcgcgcc ttcgcgactt cgcgttcgcg
     1021 ttttctaagc tacagcgcaa tctcccgatt cacatttttg taatagatgt acttcgccgg
     1081 cgtcgggccc gtcgcataaa aactttgcgg gcagtagggg cccatccaga tgaaatcgtt
     1141 gctctccact tccatccacg tgtcttcgag atagtacaca cccttgcctt gcaggaagta
     1201 caggccgtgc tccatcacgt gcgtttcgac gtacggcaga ccgtggccgg ggtcgaaggt
     1261 gaagatgttc atcgcgagat cgtattgcaa ctcgtcgggg atgagcgtct gcaggcgggc
     1321 gtgttcgttg ttgatccacg gggggccggg gacgttggat tcgtttccga ccagcggctg
     1381 gtacatctcg acgccgtcgg caggctcata gacctttcgc agtgtgagca attcgccggc
     1441 ttgtcgcatg cgcaactcgt acccgagcgt cggcggcatc agcgcgaagc tccccggcgt
     1501 gagcttccat gactggccgt cgctcgcgct cagcagcgcc tcaccggaga gcaggcagaa
     1561 gaacgtttcg acgcggccat cggcctcgtg ctgcgtaccg ccgttggctt cgaccttcaa
     1621 cagatactgc acgaactgcg cgcccagcgc cggcgccgcc agcacgaacg cttcggtttt
     1681 gtcccacgcc ggcaggcgag acgtcgggta tccctcgcgc gggaacaacg cgtatcgccg
     1741 gctcacgcgc gcacggctat gcacaagttg gttcggttct cgcatgtttt gcgtcggcgg
     1801 tccggcgttt atatcagacg cgattcacac aaacaaatcg accttgctga acgtctcaac
     1861 gtcaaccaga ccctggtcgg tcaacttcag cgcggggatc acttccaatg ccataaagct
     1921 catcgccatg aaagggtcgt gcagcgatga gcccaacttc gcggcggcgg agagcagcgc
     1981 gttgtaggcg ctacgcactt cttcaatagg acgatcgctc attaaccccg cgacgggcag
     2041 gggcagcatt gcgagcacct gctgtccttc ggcgacggcc aacccgcctt gtccttctcc
     2101 cgccgcggca cgcgcagccg tcatcattga gatgtcatcg gccccgatga cgacgaggtt
     2161 gtgatgatcg tgcgcgacgg tgccggcgat cgcgccagtc ttcagtccga agccctggat
     2221 gaagccgagg ccgatgttgc cggtgccgcg atggcgttcg atgacggcca tcttcgacag
     2281 gtcgcgcgat gcgtcggcga tcgcgaaacc gctgacgatg gacgcgggct gcacgcgatg
     2341 ttcggtcacg agctggtccg gcagagagcc gatgacgcga acgcgttcgc cccgcgcttc
     2401 gatgcgcagg tccacgcgat cccagttcac acggcaggtg ttgctgatgg cccacgatgc
     2461 cgccagtggc gtgggccgat agtcgcggat cgattggccg gcgatgaaga cctcgcgcgc
     2521 gacggggcgg cgaagatcat cgaagaccat caaatcggcc acgcgtcctg gcgcgatcgc
     2581 cccgcgatcg tgcaggccga agtattccgc cgtattcaac gtcgccaatc ggaacgcggt
     2641 gaccggatcg atgccaaatg cgatcgcttc gcgaatcatg taatcgacac cgccgtcgcc
     2701 gaggagatcg gccgcttgcc gatcgtcggt gcagaggcag atccgccggc tgttggcggg
     2761 ggtgatcagc gggagcaacg cgcgcagatt tcgagcgttc gttgcttcac ggatgaagac
     2821 gtacaggccg cgcgcgagtt tttctttcgc ttcatcgacg gcgacggatt cgtgatcgct
     2881 gccgatgccg gcggcgatgt aggcgttgag cggtgacccg cgcacgccgg ggcagtggcc
     2941 gtcgatcggc gtgcgttgaa atgccgccag cttcgccagc acgtgcgggt cacccagcat
     3001 cacgcctggg aagttcatca cttctgctaa tccgcgcacg gtgccatcgc gcagcagcgg
     3061 ggcaagatcg ttcgaggtca gctgcgcgcc gcaggtgccc atgtcggtcg cggggacgca
     3121 actgggtgcc atgatctcga tccgcagcgg cacgccgcgg cggctgtcgg ccatgaagcg
     3181 aatgccggcg acgccggcga cgttggcgat ctcgtgcgga tcggtgacga cggtggtgac
     3241 gccacgcggc atcgcggcgc cggcgaagtg cgccggaacg cacaggctgc tttcgatgtg
     3301 tacgtgcgcg tcgatcaaac cgggggcgat taaggcgttg gcgaggtcaa tcgattcctt
     3361 agccgtgtag tcggggccga tgccggcgat gtgcccgtgg gcgatggcaa tgtcggtcgc
     3421 cggctcgatc tcgccggtga agacgttgac gatgcgggcg ttgcgaagga gaagatcggc
     3481 gggggcgtcg ccgcgcgcga cggcgagcaa atcagataag ctcattctcc cgacctcgga
     3541 catgcggtgg tgaccgtgat gatatcggag cgcggcgtcg caggccacac ggggaattac
     3601 tttgatagcc tttgctagcc gcgggcgaca gccagcgggg gttcacaaca atcagcccgg
     3661 ctggtgctcg agcgcccccg cgggctgtcg cctgcggcta ttaaagatgt tcttatgatc
     3721 gttcgtcgcg cctgcgcgct cctgggaccg tcggctttgg ctcgatcttc gtctcgccct
     3781 tgcgcgtctt cgccttcccg tgaatcttcg cca
//
LOCUS       DATIMH010000709         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2189108, whole genome shotgun sequence.
ACCESSION   DATIMH010000709 DATIMH010000000
VERSION     DATIMH010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189108"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VLI90_12445"
     CDS             <1..340
                     /locus_tag="VLI90_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15060.1"
                     /translation="VGEVDPLRFLEDEFNLSFVVERRLGVGHAADGGEPAGDSGLRAG
                     LDGFLFLVAGFAEVDVDVDQTGGDVATGGVDDFVRTDGTGASASGYYVLCPKPASWKQ
                     FHLCGDAWAD"
     gene            394..1221
                     /locus_tag="VLI90_12450"
     CDS             394..1221
                     /locus_tag="VLI90_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV15061.1"
                     /translation="MHKRSWFHPELKGLVLIPAFNERQSVGKLVHRLRRALPATFDIL
                     VIDDGSTDDTLRQVPPGTAVVSLPFNLGIGGAMQTGYRYASLHGYDVAVQVDGDGQHR
                     PGEVWRLVKRLMESDSDLVVGSRFLESGRYRQSFTRWVGISMLGSLIQLLSGLRITDC
                     TSGFRVANRRVIRAFAHWYPEDYPEPEVILLLHRAGFKVTEQPVRMRQRRAGMTSIPL
                     TRGLFYVLKVITALLLDMAREPWPRGKVREHDPEPDLGDLRWEPAPAGDPAAEAVQA"
     gene            1160..1597
                     /locus_tag="VLI90_12455"
     CDS             1160..1597
                     /locus_tag="VLI90_12455"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HSV15062.1"
                     /translation="MIFGGSLLLLAIRRLKQFKLKERFTLLFMLLGLPFMVLAVWPNG
                     VEYLARISGIEQKTVMLLCVSIFLILIIFELLTIVSVMEQKITTLAQMVGILMEHQRL
                     VDRHLDGASPKRVETRKPEHPNSATPRWRSGDSAGSSSSDQAH"
     gene            complement(1574..2353)
                     /locus_tag="VLI90_12460"
     CDS             complement(1574..2353)
                     /locus_tag="VLI90_12460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV15063.1"
                     /translation="MRISIITPSFNQRKYLEQTMRSVLGQRGPFDLTWHVIDGGSIDG
                     TVSFLRSIDDPRVRWTSEPDRGQSHAINEGIDRSSDADVIGWLNSDDLYTDGALAAVA
                     DAFADPSVGWVVGRYEIIDADGLVIRSPIARYKDRFLNRYSYRKLLRENFIAQPAVFW
                     RRDFGDAVGGLDESLHYTMDYDLWLRMGKHADPRILDRVFAQFRIHTGSKSGQVNREQ
                     FDEGYRVAQRYFGGDTMSQIAHRFNVEKIVWAYRLMRLIGR"
     gene            2487..2726
                     /locus_tag="VLI90_12465"
     CDS             2487..2726
                     /locus_tag="VLI90_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15064.1"
                     /translation="MRTITPPEHQEGVVARTIEQQTAKLPSDTFLWLAGASMATSLTL
                     KLMGKDHDALFVGQWAPSFLLLGIYNKIVKVAGSD"
BASE COUNT          517 a          842 c          928 g          558 t
ORIGIN      
        1 cgtgggcgag gtcgacccgc tgcgattcct ggaggacgaa ttcaatttga gcttcgttgt
       61 cgagcgccgg cttggtgttg ggcatgcagc agacggtggt gaacccgccg gcgacagcgg
      121 cttgcgcgcc ggattggatg gtttcctctt cctcgtcgcc gggttcgcgg aggtggacgt
      181 ggatgtcgat cagaccgggg gtgatgtagc gaccggtggc gtcgatgact tcgtccgcac
      241 ggatgggacc ggggcttccg cctccggcta ttacgtgctt tgccccaaac cagcgtcgtg
      301 gaaacagttc cacctttgcg gggatgcatg ggccgattaa gatgtcatct cgcgccgccc
      361 cgtgccgcgg ggtgcggcaa tgttccgaca accatgcaca aacgatcgtg gttccatcct
      421 gaactgaaag ggttggtgct catccccgcg ttcaacgaac gccagtcggt gggcaagctg
      481 gtgcaccgcc tgcgccgcgc gctgccggcg acgttcgaca tcctcgtcat cgacgatgga
      541 tcgacggacg acacgctgcg ccaagtcccg ccgggcacgg cggtcgtcag tttgccgttc
      601 aatctcggca ttggcggggc gatgcagacg ggatatcgct acgcttcgct gcacggctac
      661 gacgtcgctg tgcaggtgga cggcgacggg caacatcgcc ccggtgaagt ctggcggctg
      721 gtcaagcgac tgatggaaag cgattcggat ctggtggtcg ggtcgcgctt tttggaatcg
      781 gggcgctatc gccaatcgtt cacgcggtgg gtcggcatct cgatgttggg ctcgttgatc
      841 caactcttga gcggattacg aatcaccgac tgcacgagcg gtttccgcgt ggcgaaccgt
      901 cgcgtgatcc gtgcgttcgc gcattggtat ccggaagatt atccggagcc ggaagtgatt
      961 ctgctgctgc atcgcgcggg gttcaaggtc accgaacaac cggtgcgcat gcggcagcgg
     1021 cgcgcgggga tgacgagcat cccgctcaca cgcggtttgt tctacgtgct gaaggtgatc
     1081 acggccctgc tgctggacat ggctcgcgag ccctggccac gcgggaaggt gagagagcat
     1141 gatccggagc ctgatcttgg tgatcttcgg tgggagcctg ctcctgctgg cgatccggcg
     1201 gctgaagcag ttcaagctta aggagcgctt cacgctgctg ttcatgctgc tcggcctgcc
     1261 cttcatggtg ctggcggtgt ggccgaacgg cgtggaatac ctcgcgcgca tcagcgggat
     1321 cgaacagaag accgtgatgc tgctgtgcgt gtcgatcttc ctgatcctga tcatcttcga
     1381 actgctgacg atcgtgagcg tgatggagca gaagatcacc acgctggcgc agatggtcgg
     1441 catcctgatg gaacaccagc ggctggtgga ccggcatctc gatggcgcat cgcccaaacg
     1501 cgtggagacg cgtaagccgg aacatcccaa cagcgccacg ccgcgttggc gcagcggcga
     1561 ttcggcggga tcttcatctt ccgatcaggc gcattaagcg gtacgcccat acgatctttt
     1621 cgacattgaa gcggtgtgcg atttgactca tcgtgtcgcc gccgaagtag cgctgcgcga
     1681 cgcggtaacc ttcatcgaat tgctcgcggt tgacctgccc cgatttgctg ccggtgtgaa
     1741 tgcggaactg ggcgaagacg cggtcgagga tgcgcgggtc ggcgtgcttg cccatgcgca
     1801 gccagaggtc gtagtccatc gtgtagtgga gcgattcgtc cagcccaccc acggcatctc
     1861 cgaaatctcg ccgccagaag accgccggtt gcgcgatgaa gttttcgcgc agcagcttgc
     1921 ggtacgagta gcggttgagg aatcggtctt tgtagcgagc gattggtgag cggatcacga
     1981 ggccgtcggc gtcgatgatc tcgtatcgac ccaccaccca accgacggaa ggatcagcga
     2041 acgcatcggc gaccgcggcg agcgcaccat ctgtatacaa gtcatcgctg ttgagccagc
     2101 cgatcacgtc ggcatcggac gatcggtcga tgccttcgtt gattgcatgc gattgccccc
     2161 ggtcgggttc gctggtccac cgtacgcgcg ggtcatcgat gctgcgaagg aacgacacgg
     2221 tgccgtcgat gctgccgcca tcgatcacgt gccacgtcaa atcgaacggg ccgcgctggc
     2281 cgagtacgga ccgcattgtc tgttcgagat acttccgctg attgaacgag ggcgtgatga
     2341 tgctgatgcg catgcggcga agcggatgat acccgcgaat tgcccgccgg gcactccatt
     2401 tgctcggcca caccccggcg cttccgccga attggctgac ggtcggccgc gggcaggagg
     2461 ctcaacgcaa tcgaaggagt ttacccatgc gcaccatcac accccccgaa catcaggaag
     2521 gcgtcgtcgc ccgcacgatc gaacagcaaa ccgccaagct gccatcggac acgttcctct
     2581 ggctcgccgg cgcgtccatg gcgacatcgc tgacgctgaa gctgatgggc aaggatcacg
     2641 acgcgctatt cgtcggccaa tgggcgccgt cgttcctgct gctcggaatc tacaacaaga
     2701 tcgtaaaggt ggcggggtcg gattaatcgg ccggaagagt gcaagaagcg taatcagccc
     2761 ccacgccggg ttcggaggta ctccgacgac ccggattctt cccgcctcga gcaagaagaa
     2821 tccaagtcgt cggagtacct ccgaa
//
LOCUS       DATIMH010000710         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3920005, whole genome shotgun sequence.
ACCESSION   DATIMH010000710 DATIMH010000000
VERSION     DATIMH010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3920005"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..516)
                     /locus_tag="VLI90_12470"
     CDS             complement(211..516)
                     /locus_tag="VLI90_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSV15065.1"
                     /translation="MKRLEGKPFAIVGVNSDSDREKIKQRIGEEHITWRSFWNNGTAG
                     PIAMKWNVFSWPTTYVLDAKGVIRFKEVRGQSLDDAVDSLLKEMGVTVAAHPPATQE"
     gene            complement(556..1419)
                     /locus_tag="VLI90_12475"
     CDS             complement(556..1419)
                     /locus_tag="VLI90_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15066.1"
                     /translation="MRHLPMVLVILLCAILARADAPPASRPAGDAAAQVAAVIAEYDK
                     AMADFSQAYAAVKTPDEQQKVMEKYPQPALYLSRLLTIAQDNPKTPSAFDALKWIIDH
                     DRAQTPTGAAALKIVTSDFAADPRAGEVAADLTWSASPAAEAFFQAVIAQNPDRTAKG
                     MATFAMGRLKKSQAGDARQIQSHSVDVNADEAFLGKDVFAAMMKMDPDALTKEAELLF
                     EKVRAEYADVKGGFHETLGKSAEAELFELRELAIGKTAPDIAGEDIAGQPMKLSDFRG
                     KVVLLDFWGDW"
     gene            complement(1455..1808)
                     /locus_tag="VLI90_12480"
                     /pseudo
     CDS             complement(1455..1808)
                     /locus_tag="VLI90_12480"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
     gene            complement(1821..2630)
                     /locus_tag="VLI90_12485"
     CDS             complement(1821..2630)
                     /locus_tag="VLI90_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15067.1"
                     /translation="MGGWLRLIAVLWLCAMPCDLFAADEPVATRVQAIVAEHARAMSD
                     FTRAYMAGGTSENRERIARESYPPVAPCSEQLLAIARQHPADPATFDALQWIVAHDGP
                     GPRFEDASMLLAGHFTRDPRAASVALVFMNSTPNSAAAEAFLSSTISQNPDRDTKGIA
                     SYALARIRQKRVGEISDADPNPNAVGLKRSRIIEVEVMLRTIQSEYADVAAGPQFTLG
                     KSADEDLFELHSPLAIGRVVPEISGQDIDGRPMKLSAFRGKVVMLDFWGDW"
     gene            complement(2795..3208)
                     /locus_tag="VLI90_12490"
     CDS             complement(2795..3208)
                     /locus_tag="VLI90_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bZIP transcription factor"
                     /protein_id="HSV15068.1"
                     /translation="MKLRITSGLVIAGLLLPMALGAEPAPPGTADSEAKLQQRIKELD
                     AYAKSLEKRVADLAKRNDELERRLNERWPQFRLVVPPGEPLVPFKQAPRVTPSPSPFG
                     APSPLPPRQPGWVPRGSGNTSHYLVPTEQPMVVGN"
BASE COUNT          580 a         1200 c         1010 g          665 t
ORIGIN      
        1 tgctctgtct cggccctctg gaccgcgccg atctttctgt agctgcgacg cctgcgtcgc
       61 cctctttttc acgaacacca gtcatgaaga agagacgacg caggcgtcgc agctacagaa
      121 atcagggtcg agacagagca ggtcgcactt attttacatc cttcgacttc gtcgcagtac
      181 cgcctgcgct gaagataaac ttcgaacgat tcactcctgc gtcgccggcg ggtgcgccgc
      241 caccgtcacg cccatctctt tcagcaggct gtccaccgcg tcgtccagcg attgcccgcg
      301 gacttccttg aaacggatga cgcccttggc gtccagcacg tacgtcgtcg gccagctgaa
      361 gacgttccac ttcatcgcga tcgggccggc cgttccgttg ttccagaacg accgccaggt
      421 gatgtgttct tcgccgatgc gctgcttgat cttctcgcga tcggagtcgc tgttgacgcc
      481 gacgatcgcg aacggtttgc cttccagacg cttcacgagc gaccgctcgt gcgggtacat
      541 gccctggcag ggccctcacc agtcgcccca gaagtccagc agcaccacct tcccgcgaaa
      601 gtcgctcagc ttcatcggct ggcccgcgat gtcctcgccg gcgatgtccg gcgcggtctt
      661 gccgatcgcc aattcgcgca gctcgaacaa ttctgcctcg gcggatttgc cgagcgtctc
      721 gtgaaacccg cccttcacgt ccgcgtattc cgcgcgcact ttttcgaaca acaattccgc
      781 ttccttggtc agcgcatcgg ggtccatctt catcatggcg gcgaagacgt ccttgcccag
      841 gaaggcttcg tccgcgttga catcaaccga atgcgattgg atctggcgcg cgtctccggc
      901 ctggcttttc ttgagccggc ccatcgcaaa cgtcgccatg cccttggcgg tgcgatccgg
      961 gttctgcgcg atgaccgcct ggaagaacgc ctcggcggcc ggcgaggccg accacgtcag
     1021 gtccgccgcc acttcgccgg cgcgcggatc ggcggcgaag tcgctggtca ctatcttcag
     1081 cgccgccgcg ccggtcggcg tctgagcgcg gtcgtgatcg atgatccact tcagcgcatc
     1141 aaacgccgac ggcgttttcg gattgtcctg cgcgatcgtc agcagccgcg ataaatacag
     1201 cgccggctgc ggatatttct ccatcacctt ctgctgctcg tccggcgtct tcaccgcggc
     1261 gtacgcctgc gagaagtcgg ccatcgcctt gtcgtattcg gcgatgaccg ccgccacctg
     1321 cgccgccgcg tcccccgccg gccggctcgc cgggggcgcg tcggcccgcg ctaagattgc
     1381 gcacaacaag atcaccaaaa ccattggcag atgtctcatg cgaatacctc ccgtgcgcac
     1441 gcgctggcgc gcgctcattc cggcggcggt ggatgcggct gcaccgtcac gcccatgtcc
     1501 ttcagcagtc cgtcaatcgc gctgtccagt ttctgtcccc gaacgtctct gaaccggatc
     1561 acgccgttgg cgtcgatcac gaagatcgac ggccattgct tgatgtgcca ttgctgcgcg
     1621 atggtctcgc ccaggacgcg cccatcggtg cgcgcgtccg gctggtccca ccacgatcgc
     1681 caggtgatgt gctcgtcctt gatgcgctgc ttgatcttct cgcgatcgga gtcgctgttg
     1741 acgccgacga tcacgaacgg tttgccttcc agacgcttca cgagcgaccg ctcgtgcggg
     1801 tacatgccct ggcaggggcc tcaccaatcg ccccagaaat ccagcatcac caccttgccg
     1861 cggaacgcgc tcagcttcat cggtcggccg tcgatgtcct ggccgctgat ctccggaaca
     1921 acccgcccga tcgccaacgg cgagtgcagc tcgaacagat cttcatcagc cgattttcca
     1981 agggtgaact gcggtcccgc cgcgacgtcg gcgtattcgg actggatcgt ccgaagcatc
     2041 acctcaacct cgatgatgcg cgaccgtttc aagccgacgg cgtttggatt tggatccgca
     2101 tcgctgattt ctcccacgcg cttctgacgg atgcgcgcca gggcgtaact ggcgatgccc
     2161 ttggtgtcgc gatcggggtt ctgcgagatc gtcgatgaca aaaacgcttc cgccgccgcg
     2221 ctgttcggcg tgctattcat aaaaaccagg gcaaccgacg cggcgcgcgg atcgcgggtg
     2281 aaatgcccgg ccagcagcat cgacgcgtct tcgaaccgtg gacccggccc atcgtgagcg
     2341 acgatccact gcagcgcatc aaacgttgcc gggtcggccg gatgctggcg cgcgatcgcg
     2401 agcagctgct cggaacaggg tgcgaccggc ggataagact cgcgcgcgat ccgctcgcga
     2461 ttctcgctcg tcccgccggc catgtacgcg cgcgtgaagt cgctcatcgc ccgcgcgtgc
     2521 tcggcgacga tcgcctgcac gcgcgtcgcg acgggttcgt cggccgcgaa cagatcacac
     2581 ggcatcgcgc acagccacag caccgcaatc aaccgcagcc aaccccccac gccgggttcg
     2641 gagggcctgc cctgagcctg tcgaaggagc ctgccctgag cctgtcgaag ggtgctccaa
     2701 cgacccggat tctttggggc acaagaagca tccgggtcgt cgcgagtacg ctccgaaccc
     2761 ggcgtggtgt gggaatgaat gcgctttatg atcatcagtt tcccaccacc atcggctgct
     2821 cggtcggaac caaatagtgc gacgtgtttc ccgaaccccg gggcacccat cccggctgcc
     2881 gcggcggcaa gggtgacggc gccccgaacg gcgacggact cggcgtgacg cgcggcgcct
     2941 gcttaaacgg caccagcggt tcccccggcg gcaccaccag ccgaaactgc ggccaacgct
     3001 cgttcaaccg ccgctccagc tcatcgttcc gcttcgccaa gtccgcgacg cgcttctcca
     3061 acgacttggc gtacgcatcc agctccttga tccgctgctg cagcttcgcc tcgctgtccg
     3121 ccgtgcccgg cggcgccggc tccgccccca gcgccatcgg cagcaatagc cccgcgatga
     3181 ccagtccgct ggtgattcgc aacttcattg gcggcctccg taggcagatg ttcctctttc
     3241 cctttaggcg cggccgaacc ggaaagttta catcagcaaa ggatgagatg ggaaagattt
     3301 cgctcagctt tatcctgagc gaagcggcgc agccgcgacg tcgaaggacc tggtggagaa
     3361 gtggatgatc cgcttcttac gggcgacaaa cagtcggcgt tatcgtgatc agcggtcatc
     3421 ggcgctcccc tcaaatccct tactccgcac gagta
//
LOCUS       DATIMH010000711         6333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5371903, whole genome shotgun sequence.
ACCESSION   DATIMH010000711 DATIMH010000000
VERSION     DATIMH010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5371903"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="VLI90_12495"
     CDS             complement(<1..1679)
                     /locus_tag="VLI90_12495"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSV15069.1"
                     /translation="MLAALLLMAMAVGCSAAPAANSPRKDVLLRDGWAFIRQDVPAAQ
                     SPQFDDSRWQRIALPHTWNAHDGQDGGNDYYRGRGWYRLHLNVDAADLRKQLFLRFEA
                     ASLVAQVYLNGQFAGTHRGGFAAFCFNVTPLLHAGDNVVAVCVDNSHFDDIPPHSGDF
                     TICGGLYRNVHLLALNAVNITPTDDGSPGVYLRTLDLAETSANVQATVKLHNDGDGTQ
                     SIVAACAIKDASGATVATAQAQQTIAARSAADAMLSLVIPHPHLWNGRADPYLYQAVV
                     ELKRGSEVLDRVVQPLGLRQYRIDPQAGFFLNGRSYPLRGVCVHQDYLNKGWAIAPAD
                     IDTNYRLINELGASAVRLAHYQHPDYEYAVCDRSGVVVWAELPMVNRIGDTAAFSENA
                     KQQLRELIKQNYNHPSICFWSLFNELGPRTRTDWQLVSQVNELAHDLDADRPTVAASH
                     LPVGIPVNWIPDLIAFNRYFGWYSGTIADWPRELDALHAARPDRAIGISEYGAGASVN
                     QHQDRMTTRPSTKGPWHPEEWQALVHEAAYRAIQQRPWLWGTFVWVMFDFAS"
     gene            1938..>6333
                     /locus_tag="VLI90_12500"
     CDS             1938..>6333
                     /locus_tag="VLI90_12500"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV15070.1"
                     /translation="MFHLLRGRAILLAMCLLISGARANAQIDEAQFKSDLNAIAAAPS
                     RVIGSSGYEQAGRYLESEIGKLPHVELKRQDFSVVVPVTQSATLDIGSGRIEAVYPFW
                     PAHVRVCSTPPEGIRGNLVYVGQCRYEDLKPASLAGQIAVIEAAAGSRWSEPFYLGAK
                     AILVLGSPDTGWPDLKDHDLRIPVSMPRFYVPPGALADDLRAGRIADATLKATVQWQP
                     KRAVNYYALVPANKPTPSQSPGALMFSVPYDASSLVPDLAPGAGQAVQAAAGLALLRD
                     LSRHPLDRPVIVFFSGADTIQFLGTRNMFLALGTAPATWNDELATLNDKLADAQRDLA
                     RATELMDAPQRLSPVDDRRLVGWIVEMIDTDLADAQDQLFRLRSLRPADQTADTRRDL
                     ASLDDRQAALNRLKLAVAQHPAELMDANQSAATDVMKRVAARLQGLLLSDQSRKDDLQ
                     KRIALHDWLADAETGHRDATHRLIELLVGLDLSDHGSRAGPMFLGYFQRASSLPQVQL
                     YNDWFAKLERSFAAHTAGSEWWGAVRGVVDLDTINQTRAPVTYLAGPLPLASELAQAW
                     GTPGMSMITLDDMRLRRDTPTDTLANLNVNPILRQLIGVRELFWHAWNDPKFRGPIEL
                     KQLNVSFAGQVVGAAPGKPVPDLPRPGFLAAYYYLQTAGASHKIPRLGILPWTLGIRR
                     DEVRDCDAEGNYRFEGMPRMRSDRLEGGSSREQNDMQVFAVHVYRFDSASGAITATTD
                     LGKQSGDAGWAVDIKQDVPLVRSLAFNCQEFSLSGLYDPRYLQTLSEMVPIDARRDAE
                     PQRYCMWLDNKMLAGFVEPGTRNALLIRFGRVGNRLILVNMAERSNAKGELDEGRGYS
                     VDQLNHIGPLALATSRDFYRLNDDRLTNYRRAGVSSALIDSLHDSAARQLRDAERSLS
                     GDDDVGLTRESTGAWANEARVYDAAQEMARDVVRAAIFLLMLCVPFSFCMERLLIATP
                     NVYKQIAGIGAIFLVMTLALWTFHPAFKISASPLIIVLAFAIIAMSLLVIFVIYGKFD
                     TELKRMRSGRGVAQGATFANAGVLMSAVLLGIANMRKRKFRTVLTSITIVLITFAVLW
                     FTSTAHYRGTTALPTGVASAHPGILLRQRGFRPMAELVTDQLRAVLADPALNLGAPPQ
                     VVERWWAVSAADPKEQYDLVVPASGAKPSRTVAVQSVLGLSPGESQLTRIAEVVGANQ
                     FARLENGERSVIYLSDIAADELKVREGDRIRLGGTDLQVAGIFSAAAFDQKVAMLSGE
                     SIAPLKYLAGELDAGGRLMNETNSDALELSGSASAAEAGSIYEHLPASQMVIVPASVC
                     RTLNNSSLRCVAFRLQDQKQVEAVSDELTRRFALAMYAGFDDGVRMVAAGNLASVSGA
                     SQVAVPLAIAGLIIFNTMMGSIAERRREIHVYTSLGLAPMHVGALFVAEAMTYGLVGT
                     VFGYIIGQGLGTLMLKMGWLGSITLNYSG"
BASE COUNT         1119 a         2058 c         2066 g         1090 t
ORIGIN      
        1 gatgcgaagt caaacatcac ccacacgaac gtgccccaga gccatggccg ctgctggatc
       61 gcgcgatacg ccgcctcgtg caccagcgcc tgccattcct ccggatgcca cgggcctttc
      121 gtcgatggcc gggtcgtcat gcggtcctgg tgctgattga cgctcgcgcc ggcgccgtat
      181 tcgctgatgc cgatcgcccg gtccggccgg gccgcgtgca gcgcatccag ctcgcgcggc
      241 caatcggcaa tcgtcccgct gtaccaaccg aagtaccggt tgaacgcgat cagatctgga
      301 atccagttga caggaatccc caccggcaga tgcgacgccg ccaccgtcgg tcgatccgca
      361 tccaggtcat gcgccagttc attcacctga ctcacgagct gccaatccgt ccgcgtgcgc
      421 ggccccagtt cgttgaacaa actccagaag cagatcgacg ggtgattgta attctgcttg
      481 atcagctcgc gcagctgctg cttggcgttc tcgctgaacg ccgcggtgtc accgatgcga
      541 ttcaccattg gcagctcggc ccagacgacg acgccgctgc ggtcgcacac ggcgtactca
      601 taatcaggat gctggtaatg cgccagccga accgccgagg cgcccagctc gttgatcaac
      661 cggtaattgg tgtcgatgtc ggcgggcgcg atcgcccatc ctttattgag atagtcctga
      721 tgcacgcaca cgccgcgaag cggataggac cggccgttga ggaagaaccc cgcctgcgga
      781 tcgatgcggt attgccgcag gcccagcggt tgaacgacgc gatcgagcac ttcgctgccg
      841 cgtttcagct cgacgaccgc ttgatagaga tacgggtcgg cccgcccgtt ccacaggtgc
      901 ggatggggaa tgaccagcga caacatcgca tccgccgccg agcgggcggc gatcgtttgc
      961 tgagcttgtg cggtggcgac ggttgcgcct gaagcgtctt tgatggcgca cgccgcaacg
     1021 atgctttgcg tcccatcacc gtcgttatga agcttcaccg tcgcctgaac gttcgccgat
     1081 gtttcggcca agtccaacgt cctgagatac acgccgggcg agccgtcgtc agtgggggtg
     1141 atgttgaccg cgttcagcgc cagcaggtga acgttgcgat agagtccgcc gcagatcgtg
     1201 aaatcgccgg agtggggcgg gatatcgtcg aaatgcgagt tatcgacgca gacggcgacg
     1261 acgttatcgc ccgcgtgaag cagcggggtg acattgaagc aaaacgccgc gaacccaccg
     1321 cggtgggtgc cggcgaactg gccgttgagg taaacctgcg ccaccaggga cgcggcttcg
     1381 aaccggagaa agagctgctt gcgaagatcg gcggcatcga cgttcaagtg cagccgatac
     1441 catccccggc cgcggtaata gtcgttcccg ccatcctgcc cgtcgtgcgc gttccacgtg
     1501 tgcggcagcg cgatgcgctg ccagcgcgaa tcatcaaact gcggcgactg cgccgcggga
     1561 acatcctggc ggatgaacgc ccagccgtcg cggagcaaca catccttgcg cggcgagttc
     1621 gccgctggcg ccgcgctgca accgacagcc atcgccatca acagcaaggc ggcgagcatt
     1681 cgtacaatcg atcgggcggc gtgtgaaagc atgtgacgca tcacaatcgc cgcgcgcgcg
     1741 caacgggtca aggaagtcaa ctcgtagcac gggcgccaga acccctgtcg agcacttagg
     1801 cacgggctct ggcgcccgtg ctacgaaatg cgcacgcgac ctgaatgaat gctgcaagcg
     1861 aaaggacagg aaagacaacg catttgcaga agcgcgatcg ttgtgcggtt cgggtggtca
     1921 tcgcaaaatg agtggctgtg ttccacctcc ttcgtggccg ggcgatcctt ctggcgatgt
     1981 gcctgctgat ttcgggcgcg cgcgccaatg cccagatcga tgaagcgcaa ttcaagtcgg
     2041 acctgaacgc gatcgccgcc gcaccgtcgc gcgtgatcgg ctcgagcgga tacgaacagg
     2101 cggggcgata tctcgaaagt gaaatcggga agctgccgca cgtcgagctg aaacggcaag
     2161 acttttcggt ggtggtgccg gtaacccagt cggcaacgct cgacattggg agcggacgca
     2221 tcgaagccgt gtatccgttc tggccagcgc acgtgcgcgt ctgctcgacg ccgcccgaag
     2281 gcatccgcgg caacctcgtg tatgtcggcc agtgtcgcta cgaggacctg aagcccgcgt
     2341 cgctcgcggg gcagatcgcc gtgatcgagg ccgccgccgg cagccgctgg tccgagccat
     2401 tctatctcgg ggcaaaggca atccttgtcc tcggcagccc cgacacgggc tggcccgatc
     2461 tcaaagatca cgacctgcgc atccccgtga gtatgccgcg gttttacgtt ccgccgggcg
     2521 cgctggcgga cgatctgcgc gccgggcgga tcgcggacgc gacgctgaag gcgacggttc
     2581 agtggcagcc gaagcgggcc gtcaattact acgcgctggt ccctgccaac aagccgaccc
     2641 cgtcgcaatc gccgggcgcg ttgatgttca gcgtgccata cgacgccagt tcgctcgtgc
     2701 ccgacctcgc gcccggcgcc ggacaggccg tgcaggccgc cgcgggtttg gcgctgctgc
     2761 gcgatctgtc acgtcacccg ctcgatcgcc cggtgatcgt cttcttcagc ggcgccgaca
     2821 cgatccaatt cctcggaaca cgcaacatgt tcctcgcgct cggcaccgcg ccggcaacgt
     2881 ggaacgacga gctggccacg ctgaacgaca agctcgcgga cgcacagcgc gacttggccc
     2941 gcgcgacgga gctaatggac gcgccgcagc ggctgtcgcc ggtggatgac cgccggctgg
     3001 tgggatggat cgtcgagatg atcgacaccg atctggccga tgcgcaggac cagctattcc
     3061 gcctgcgcag cctgcggccc gccgatcaaa ccgccgacac ccgccgcgac cttgcttcgc
     3121 tggacgaccg gcaggcggcg ctgaatcgat tgaagcttgc ggtcgcgcag cacccggcgg
     3181 agctgatgga cgccaatcaa tctgcagcca ccgacgtgat gaagcgcgtc gctgcgaggt
     3241 tgcagggttt gctcctgtcg gatcagtcgc gcaaagatga cctccagaag cgcatcgcgc
     3301 tgcacgactg gctcgccgac gccgagaccg gccatcgcga cgcgacccat cgcctgattg
     3361 aattgctcgt cggcctggac ttgtccgatc acggcagccg cgctgggccg atgtttctgg
     3421 gttatttcca gcgcgcctcc agtctgccgc aggtccagct gtacaacgat tggtttgcga
     3481 agctcgaacg cagtttcgcc gcgcacaccg ccggcagcga gtggtggggc gctgttcgcg
     3541 gcgttgtcga tctcgacacg atcaaccaga cgcgcgcccc ggtgacatac ctcgccggcc
     3601 cgttgccgct ggcgtcggaa ctggcgcagg cgtggggcac gccgggcatg tcgatgatca
     3661 cgctggacga catgcgcctg cgccgcgaca cgccgaccga tacgctggcc aacctgaacg
     3721 tcaacccgat ccttcggcaa ctcatcggcg tccgcgaact cttctggcat gcgtggaacg
     3781 atcccaagtt ccgcggacca atcgaactga aacagctcaa cgtcagcttc gccggtcagg
     3841 tcgtcggtgc cgcgccgggc aagcccgtgc cggatctccc gcggccggga tttctggcgg
     3901 cgtactacta ccttcagacc gccggcgcca gccacaagat cccccggctc ggcatcctgc
     3961 cgtggacgct cggcatccgc cgcgatgagg tgcgggactg cgacgccgag ggaaactacc
     4021 gcttcgaagg catgccgcgg atgcgcagcg atcgcttgga aggcggctcc agccgcgagc
     4081 agaacgacat gcaggtcttc gcggtgcacg tctaccgctt cgattctgcc agcggcgcga
     4141 tcaccgcgac gacggacctc ggcaaacaat ccggcgacgc cggttgggcg gtcgatatca
     4201 agcaggatgt tccgctggtc cgcagcctgg cgttcaactg ccaagagttc agcctgagcg
     4261 ggctgtacga cccgcggtat ctgcagacgc tcagcgagat ggtgccgatc gatgcgcggc
     4321 gcgacgccga gccgcagcgg tattgcatgt ggctggacaa caagatgctg gccggcttcg
     4381 tcgaacccgg cacgcggaac gcgctgctga tccgcttcgg ccgcgtcggt aaccggctga
     4441 tcctcgtcaa catggccgag cgttcgaacg caaagggcga gcttgacgaa ggccgcgggt
     4501 acagcgttga tcagctgaac cacattggcc cgctggcgct ggcgacgtcg cgggatttct
     4561 atcgcttgaa cgacgaccgg ctgacgaact atcgccgcgc cggtgtctcc agcgcgctga
     4621 tcgacagcct gcacgacagc gccgctcggc agctgcgcga cgccgagcgg tcgctgtcgg
     4681 gcgatgacga tgtcggcctg acgcgcgaat cgaccggcgc ctgggccaac gaagcgcgtg
     4741 tctacgacgc cgcccaggag atggctcgcg atgtcgtgcg cgcggccatc ttcctgctaa
     4801 tgttgtgcgt ccccttttcg ttctgcatgg aacgactgct catcgccacg ccgaacgtgt
     4861 acaagcagat cgccggcatt ggcgcgatct tcctggtgat gacgctggcg ctgtggacgt
     4921 ttcacccggc gttcaagatc agcgccagcc cgctgatcat cgtgctggcg ttcgcgatca
     4981 tcgcgatgtc gctgctggtc atcttcgtga tctacggcaa gttcgacacg gaattgaagc
     5041 gcatgcgctc cggccgcggt gtggcgcagg gggcgacgtt cgccaacgcc ggcgtgctga
     5101 tgagcgcggt gctgctgggc atcgccaaca tgcgcaagcg gaagttccgc acggtgctga
     5161 ccagcatcac gatcgtgctg atcacgttcg ccgtgctttg gttcaccagc acggcgcact
     5221 accgcggcac cacggcgctg ccaacgggtg tggcgtcggc gcatcccggc atcctgcttc
     5281 gtcaacgcgg gtttcgaccg atggcggaac tggtgacgga tcagcttcgc gcggtgctgg
     5341 ctgatccggc gctgaacctg ggcgcgccgc cgcaggtggt cgaacgctgg tgggccgtct
     5401 cggcggccga cccgaaggag cagtacgacc tggtcgttcc cgccagcggc gctaagccct
     5461 cgcgcacggt cgcggtgcag tcggtgctcg ggctgtcgcc gggtgaaagt cagctgacgc
     5521 ggatcgcgga agtcgtcggg gcgaatcagt tcgcgcgcct tgagaacggc gaacgcagcg
     5581 tcatctatct cagcgacatc gccgccgatg aactgaaggt gcgcgaaggc gatcgcattc
     5641 gcctcggcgg gaccgatctg caggtggccg gtatcttcag cgccgctgca ttcgatcaga
     5701 aggtcgcaat gctcagcggc gaatcgatcg cgccgctcaa atacctcgcc ggcgaactgg
     5761 atgccggcgg gcgattgatg aacgagacga attcggacgc gctggagctg agcggcagcg
     5821 ccagcgccgc cgaggccggc agcatctacg aacacctgcc ggcgtcgcag atggtgatcg
     5881 tcccggccag cgtctgccgg acgctcaaca acagctcgct gcgctgcgtt gcgtttcgac
     5941 tccaagatca gaagcaggtt gaagcggtga gtgatgaact gacccggcgc ttcgcgctgg
     6001 cgatgtacgc cggcttcgac gacggcgtgc gcatggtcgc cgccggaaat ttagcgagcg
     6061 tcagcggcgc gtcgcaggtc gcggtgccgc tggctatcgc ggggctcatc atcttcaaca
     6121 cgatgatggg ctcgatcgcc gagcgccgac gggaaattca cgtctacacg tcgctgggcc
     6181 tggcgccgat gcacgtcggc gcgctcttcg tcgccgaggc gatgacctac ggcctggtcg
     6241 gcaccgtctt cggttacatc atcgggcagg ggcttggcac gctgatgctg aagatgggct
     6301 ggctgggcag catcacgctg aattactccg gca
//
LOCUS       DATIMH010000712         4630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_170007, whole genome shotgun sequence.
ACCESSION   DATIMH010000712 DATIMH010000000
VERSION     DATIMH010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170007"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VLI90_12505"
     CDS             <1..1063
                     /locus_tag="VLI90_12505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV15071.1"
                     /translation="FWGPSIICAFVAIGMAISVKETPEEHGYLPVEDYAGGRPGKNVF
                     LFGLILTVLLVSVASWIGWLYHTSRIGPMDLGVIVIVFIIAVFILIHILAARNPPPPL
                     EVLAPPIVAPADPPNVEVVPAGSAVPLGYVSAVRNPKAYDERATRADIQVALRTIVRN
                     PAVWIVAAAYACTGAVRNPIDSWFARYMQEAQHTSDTSSLFQVLAFLIPCAGSAGSLL
                     SGYISDKLFAGARSPIAAALYGIETCFVALAVFFVKSPQSAVFFLVALSFTANATHSI
                     LGTAAAMDIGGRKMTGFASGLIDSFQYFGATLGLWALGHLLDRYGWRALFPYMIPFGV
                     IGFLLMVFGRGIIARGSKE"
     gene            1093..2523
                     /locus_tag="VLI90_12510"
     CDS             1093..2523
                     /locus_tag="VLI90_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSV15072.1"
                     /translation="MMRRYAFLLLFLAVLVTPFVLRLAMGVSQAAQSRDANALPLVII
                     TANVESARREFADAFSAWHAQKFGQRVYVDYRVYGAGDIVKYFDASRDTIFRTQGTYK
                     VDLAWGGGDYLFEQQLKKPGYLEGVAIDAAIFHAAFPKPDLNGVPLYDVASNPPQWYG
                     TALSSFGIAFNRDVDRYLKRPDPSTWNDLADPAYRGWIVAADPTRSASAKQSFLAIVE
                     KAMADASAAGEREDLGWARGMGRIRRIAANARLFTDSASAVPGIISSGDAAAGMAIDF
                     YGRSQAEAVGSQLMGYVEPKGATVISPDPIALVRGAEHRELAVRFIEFVLSDQGQRLW
                     NTRAGAPGGPRQSSLRRLAISPAVYGDMSNFTDPVNPYTAAGGFNKSPAREKTFGIIG
                     ELIQASCIDPLDDLRETRRIILASPRAAELDAKLGMFPFDQKEALSRGAKYRAASAID
                     RLAMMRNWTAEFRAEYRRLREEAVAP"
     gene            2520..2801
                     /locus_tag="VLI90_12515"
     CDS             2520..2801
                     /locus_tag="VLI90_12515"
                     /inference="COORDINATES: protein motif:HMM:NF018644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HSV15073.1"
                     /translation="MTHLRPLPLIVLVYAAASLITFIVYGWDKRRSVRGGRRVPEQTL
                     HILELLGGWPGALIAQRYFRHKWNKGSYMLAFWTIVVVHITAWALWLTR"
     gene            2997..>4630
                     /gene="typA"
                     /locus_tag="VLI90_12520"
     CDS             2997..>4630
                     /gene="typA"
                     /locus_tag="VLI90_12520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HSV15074.1"
                     /translation="MNHVRNDIRNVAIIAHVDHGKTTLVDALLRQSGNFRASQTEQDT
                     LLDSNPLERERGITILSKNVAINYTDPATNVVTKINLIDTPGHADFGGEVERVLSMAD
                     GVFLLVDAAEGPMPQTRFVLKKAFQHELRPIVVINKIDRPDSRAHEVLDMVFELFMEL
                     GADDETLDFPVLYASGRAGTASWSLEKPGTDIVPVFEALLKHVPAPHGDPEKSLQLQV
                     TSILYNDYVGRIGVGRIYNGKIKTGQQLAVVKRDGSIVKTKVAQLQSFDGLGRKNVDD
                     AEAGDIVALIGLESVDISDSICDPLNPQPLEFQDIEPPTLTMMFSVNDSPFVGKEGKY
                     VTSRNLRERLFKELESNVSLKVLETEVRDAFRVSGRGLLHLGILIENMRREGYELSIS
                     KPHVINKRAEDGTIQEPIEYLVIDVPEANLGPVMALVGNRRGELVRQDNRLGQVHLEF
                     TIPARGLIGLRTRMMTATQGQAIMHHNFHEYAAVRGDIPGRTNGVMISNCNGKANAFS
                     MNTLQDRGVLFVEPNDEVYEGQIVAENCRPDDIVVNP"
BASE COUNT          857 a         1435 c         1433 g          905 t
ORIGIN      
        1 gttttgggga ccgtccatca tctgcgcgtt cgtcgcgatc ggcatggcca tcagcgtgaa
       61 ggaaacgccg gaggagcatg gctatctgcc ggtcgaggat tacgccggtg gccggcctgg
      121 caagaacgtg ttcctcttcg gtttgatcct gaccgttctt cttgtgagcg tcgcgtcgtg
      181 gatcggttgg ctctatcaca ccagcagaat tggccccatg gatctgggcg tgatcgtgat
      241 cgtcttcatc atcgcggtgt ttatcctgat tcacatcctg gcggcgcgca atccaccgcc
      301 gccgctcgag gtgttagcgc caccgatcgt cgcgcctgca gatccgccga atgttgaagt
      361 agttcccgcg ggatcggcgg tcccgctcgg ttatgtctca gcggtgcgaa atcccaaggc
      421 gtatgacgag cgcgcgacgc gtgctgatat ccaagtcgcg ttgcgcacca tcgttcgcaa
      481 ccctgctgtc tggatcgtgg ctgccgctta tgcttgcacc ggcgcggtgc gaaatccgat
      541 cgacagctgg ttcgcccggt acatgcagga agcgcagcac acctctgata cgtcctcgct
      601 gtttcaggtg ctggcgtttc tgattccctg tgcaggatcg gccggttcac tattgtcagg
      661 ttacatctcc gataagctgt tcgccggtgc acgatcgccg attgctgcgg cgctctatgg
      721 catagagacc tgcttcgtcg cgctggcggt gtttttcgtg aaatctccac aaagcgccgt
      781 ctttttcctc gtcgccctca gcttcaccgc caacgcaacg cattccatcc tgggcaccgc
      841 tgccgccatg gacattggcg ggcgaaagat gacgggcttc gcgtcgggtt tgattgattc
      901 cttccaatac ttcggcgcga cgcttgggct gtgggcgctg ggccacctgc tcgaccgata
      961 tggctggcgc gcgctgttcc cctacatgat tccgttcggc gtgatcggtt tcctcctgat
     1021 ggtcttcggc cgcggcatca tcgcccgcgg atcgaaggag tgagcatcgc gcgatcgaat
     1081 cgcggacacg ccatgatgcg ccgatacgcg tttctcctgc tcttccttgc agtgctggtc
     1141 acgccgttcg tgcttcggct ggcgatgggc gtttctcaag cagcgcaatc gcgcgacgcc
     1201 aacgcgttgc cgctggtgat catcacggcg aacgtcgaaa gcgcccgccg cgaattcgcc
     1261 gacgcttttt cggcttggca tgcacagaaa ttcggccagc gcgtgtatgt cgattaccgc
     1321 gtctatggcg cgggcgacat tgtcaaatac ttcgacgcca gccgcgatac gattttcagg
     1381 acgcagggca cgtacaaagt cgatctcgcc tggggcggcg gcgattacct gttcgagcaa
     1441 cagctgaaga agccgggtta tttggaaggc gtcgccatcg acgcggcgat ctttcacgcg
     1501 gcgtttccca agccggattt gaacggcgtg ccgttgtatg acgtggcatc gaacccgccg
     1561 caatggtatg ggaccgcgct cagcagcttc ggcatcgcgt tcaaccgcga tgtcgatcgc
     1621 taccttaaac gtcccgaccc gagcacctgg aacgacttgg cggaccccgc gtatcgcggg
     1681 tggatcgtcg cggcggatcc gacgcgcagc gcatcggcca agcagtcctt tcttgcgatc
     1741 gtagaaaaag ccatggccga cgcgtctgcg gcgggggagc gcgaagacct cggctgggcc
     1801 cgcgggatgg ggcggatccg gcggatcgcc gccaacgcgc gcctgttcac cgattcggcg
     1861 tcggcggtgc ctggcatcat cagctccggc gacgccgccg cgggaatggc catcgatttt
     1921 tacgggcgat cgcaggcgga ggccgtcggc tctcaattga tgggatacgt cgaacccaaa
     1981 ggcgcgacgg tcatcagccc ggatccgatc gcgctggtgc gcggcgcgga gcaccgcgag
     2041 cttgcggttc gattcatcga attcgtcctg agcgatcaag gccagcgcct gtggaacacc
     2101 cgcgccggcg cacccggcgg tccgcggcaa tcatccctgc ggcggctggc gatcagtccc
     2161 gcggtttacg gcgacatgtc gaacttcacc gacccggtca acccctacac cgccgcgggt
     2221 ggtttcaata agtcgcccgc gcgcgaaaag acgttcggca tcatcggcga gttgatccaa
     2281 gccagctgta tcgatccgct ggacgacctg cgcgaaacgc ggcgaatcat cctcgcttcg
     2341 ccgcgtgcgg cggaactcga cgcgaagctg gggatgtttc cgttcgatca gaaagaagcg
     2401 ctgtcgcgcg gcgcgaaata ccgcgcggcc agcgcgatcg atcgcctggc gatgatgcgg
     2461 aattggacag ccgagtttcg cgccgagtat cgccgcctgc gcgaggaggc ggtcgccccg
     2521 tgacgcacct tcgcccgctg ccgctgatcg tcctcgtcta cgccgccgcc agcctcatca
     2581 cgttcatcgt gtacggctgg gacaagcgcc gatccgtccg cggcggtcgc cgcgtgccgg
     2641 aacagacgct gcacatcctg gaactgctcg gcggctggcc gggcgcgctg atcgcgcagc
     2701 gttacttccg ccataagtgg aacaagggca gttacatgct ggcattttgg acgatcgtcg
     2761 tcgtccatat caccgcgtgg gcactgtggt tgacgcggtg actccgcaac accccgccac
     2821 tttgcgcaaa cctcttcgcg tccgcatcgt tgtataatag tgggttggct attatcctag
     2881 ctggctcata cgcctgcgcc gttgtcccct cgttcattcc acaagtggtg aattggacgc
     2941 ctgttaggat aatccagcaa cggacgacgg actattggac aacggacaca acagtaatga
     3001 atcacgtgcg taacgacatc cgaaatgttg ccatcatcgc ccacgtcgac catggaaaga
     3061 cgacgctggt ggacgctctg ctccgccaga gtgggaactt ccgcgccagc cagaccgagc
     3121 aggacaccct gctggacagc aacccgctgg agcgcgaacg cggcatcacc attctgtcga
     3181 agaacgtcgc gatcaactac accgacccgg ccaccaacgt cgtcacgaag atcaacttga
     3241 tcgacacacc cggccacgcg gacttcggcg gggaagttga gcgcgtgttg agcatggccg
     3301 acggtgtgtt ccttctcgtc gacgccgctg aagggccgat gccgcagacg cgcttcgtgt
     3361 tgaaaaaggc gttccagcac gagcttcgtc cgatcgtggt catcaacaag atcgatcgac
     3421 ccgattcccg agctcacgaa gtgctcgaca tggtgttcga gctgttcatg gagttgggcg
     3481 cggacgatga gacgctggac ttcccagttc tgtacgcgtc gggtcgggcg ggcacggcat
     3541 cgtggtcgct cgaaaagccg ggcaccgata tcgtcccggt ttttgaggcg ctgttgaagc
     3601 acgtgccggc gccgcatggc gatccggaga agtcattgca gctgcaggtc accagcattc
     3661 tttacaacga ctacgttggc cgaattggcg tcggtcgcat ctacaacggt aagatcaaga
     3721 ccggccagca gctggcggtc gtcaagcgcg acggcagcat tgtcaagacg aaggtggcgc
     3781 agctgcagtc gttcgacggc ctgggtcgca agaacgtgga cgacgccgaa gccggcgata
     3841 tcgtcgcatt gatcggcctc gaatcggtgg acatcagcga cagcatctgc gacccgctga
     3901 atccgcagcc gctggagttc caggacatcg aaccaccgac gctgacgatg atgttcagcg
     3961 tgaacgattc gccgttcgtc ggcaaggaag gcaagtacgt caccagccga aatctgcgcg
     4021 aacggctgtt caaggaattg gaatcgaacg tctcgctgaa ggtgctggaa accgaagtcc
     4081 gcgacgcctt ccgcgtgtcc ggccgcgggt tgctgcacct gggcatcctg atcgagaaca
     4141 tgcgccgcga agggtatgag ctgtcgatca gcaagccgca cgtgatcaac aagcgcgccg
     4201 aagacggcac gattcaggag ccgatcgagt atctcgtcat cgacgtgccc gaggcgaacc
     4261 tgggcccggt gatggcgctg gttggcaatc gccgcggtga actggttcgg caggacaacc
     4321 ggctcggcca ggtgcacctg gaattcacga tccctgcgcg cggcctgatc ggtctgcgca
     4381 cgcgcatgat gaccgcgacg cagggtcagg ccatcatgca ccacaacttc cacgaatacg
     4441 ccgcggttcg cggggacatc ccgggtcgca ccaatggcgt gatgatctcc aactgcaacg
     4501 gcaaggccaa cgcgttctcg atgaacacgc tgcaagaccg tggtgtgctg ttcgtcgagc
     4561 caaacgatga agtgtacgaa ggccagatcg tcgccgagaa ctgccgcccg gacgacatcg
     4621 tcgtcaaccc
//
LOCUS       DATIMH010000713         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2768403, whole genome shotgun sequence.
ACCESSION   DATIMH010000713 DATIMH010000000
VERSION     DATIMH010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2768403"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..976)
                     /gene="holA"
                     /locus_tag="VLI90_12525"
     CDS             complement(20..976)
                     /gene="holA"
                     /locus_tag="VLI90_12525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HSV15075.1"
                     /translation="MPTATANKPVHALVGSDSFLQLQKLALILDRAPKGSQRIDIDGE
                     RADLAEVLDELRSFAMFGDGKVVVVQNADAFLTRFREQLEEYVAHPSDSATLVLRLSS
                     LPSNQRIYKAIAKTGQIEPCEPPKDLAKWITEQAKQAHQVTISPDAARMLADLIGDDM
                     GRLDNELAKLALSATNGKIDPENVATSVAFQREQQMSEMTNALAAGRAADAVKKWRQL
                     IQGDPSAEFRAVTWLAIWLTNVRKALAMRKQGMQPFAIAQALRIWPREMQGPFFETAQ
                     ALGDRGVARAMDLLAEIDRQSKSGVGDAAENVERFILSMALT"
     gene            1226..1975
                     /locus_tag="VLI90_12530"
     CDS             1226..1975
                     /locus_tag="VLI90_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15076.1"
                     /translation="MSNPFAAFCDDFYVNMRLGSQLNLPHQRETLLHFFERIQKEFPA
                     MSRFRKNDAGEFNLEEDRSSHSYRWASVEPKRLASGHVNPTSIEESLRIHTLMLQLAP
                     YHLGISPIEVDYLDVLFGFDLSFTGNHDEIIADSLFPESPLACLTEESGAKAVDFQPT
                     VTVTLSEDCRLQARIDVVTRTNSYQVRTGDYSDDVISVYLILRRYWGDRPKEPMEKIM
                     AQMSERGEQLCNQYIIPRVLKPISSAIASRS"
     gene            2248..2511
                     /locus_tag="VLI90_12535"
     CDS             2248..2511
                     /locus_tag="VLI90_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15077.1"
                     /translation="MLSADEHELLVQARQRTLALLESLEQSRAQLSDPMWSIDAATAA
                     EGRVSYTAVIDAARGTLDNLDRALSGNEDRTSNSAAAATASET"
     gene            2516..>2900
                     /locus_tag="VLI90_12540"
     CDS             2516..>2900
                     /locus_tag="VLI90_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15078.1"
                     /translation="MPEGPPPYTGGVIIASAAKDYRLKRYALVGILLIYGLLSIKDGF
                     YRYPRENAEAEQKYPGTKPPHPGFDVQLNQGLGILLPPLSIAFLIWCLYSSRGHYRFD
                     GQTLTVPGHPPVPLNALRRIDRAKWD"
BASE COUNT          550 a          925 c          869 g          556 t
ORIGIN      
        1 gtcttggcgg ccttggcgct caggtcagag ccatcgacag gatgaaccgc tcgacgttct
       61 ccgcggcgtc gccgacgccg gatttgcttt ggcgatcgat ctcggcgagc aggtccatcg
      121 cccgggcgac gccgcgatcg cccagtgctt gggcggtttc gaagaagggg ccttgcatct
      181 cgcgcggcca aattcgcaat gcctgcgcga tggcgaaggg ttgcatgcct tgcttgcgca
      241 tcgccagcgc tttgcggacg ttggtcagcc agatcgccag ccaggtgacg gcgcggaact
      301 cggccgatgg atcgccttga atcaactgcc gccatttttt gacggcatcc gcggcgcgac
      361 cggcggccag cgcgttggtc atctcggaca tttgctgttc gcgctgaaac gcgacgctgg
      421 tggcgacgtt ttccggatcg atcttgccgt tggttgccga cagcgccagc ttcgccaatt
      481 cattgtcgag ccggcccatg tcatcgccga tcaggtccgc cagcatgcgc gccgcatcgg
      541 ggctgatcgt cacctgatgc gcctgcttcg cctgctctgt aatccacttc gccaaatctt
      601 tcggcggctc gcacggttcg atctgaccgg tcttggcgat cgccttgtag atgcgttggt
      661 tcgacggcaa cgaactcagc cgcagcacca gcgtagcact gtcagacgga tgggccacgt
      721 actcttccag ctgctcgcga aagcgcgtga gaaacgcgtc ggcgttctgc acgacgacga
      781 ccttgccgtc gccgaacatc gcaaagctgc gcagctcgtc gagcacttca gccaggtcgg
      841 cgcgctcgcc atcgatgtcg attcgctgcg accctttggg cgcgcgatcg aggatcaacg
      901 ccagcttctg caactgaagg aacgagtcgg aaccgacgag cgcatggacc ggtttgttcg
      961 ccgtggcggt gggcatgtcg cggtctcttc tatgcccgtc atcgcgtgtc gtcaaattgt
     1021 caaaacgaac ccgcgggcgg tgactttcgt tctaagagga atcggctaca ctccgaacgg
     1081 gcaaccggcc gataacaatt gatggtctga taaccatctt gcgcatacgc gctgcggcgc
     1141 tgacgcgctc cagatgttca gcacggatgc gatcgcgctg cggagcagcg cgatctgaac
     1201 gggctcgggt tattgggaga cacagatgtc caatcctttc gcggcgttct gcgatgattt
     1261 ttacgtcaac atgcggctcg gttctcaact gaacctgccg caccagcgcg agaccttgct
     1321 gcatttcttc gaacggattc agaaggaatt cccggcgatg tcccgcttcc ggaaaaacga
     1381 tgcgggcgag ttcaacctcg aggaagaccg cagctcgcac agctatcgct gggccagcgt
     1441 cgagcccaag cggctggcca gcggtcacgt caatcccaca agcatcgaag agtcgcttcg
     1501 catccacacg ctgatgctgc agctggcccc gtatcacctg ggcatcagcc cgatcgaagt
     1561 cgattacctc gacgtgctct tcggcttcga cctgtcgttc accggcaacc acgacgagat
     1621 catcgccgac agcttgttcc cggaatctcc gttggcttgc ctgaccgaag agagcggcgc
     1681 caaggccgtc gatttccagc cgaccgtcac cgtgacgctt agcgaagact gccgattgca
     1741 ggcccgcatc gatgtcgtca cgcgcaccaa cagctatcag gtccgcaccg gcgactacag
     1801 cgatgatgtc atcagcgtct acttgatcct ccgccgctat tggggcgatc gtcccaaaga
     1861 accgatggaa aagatcatgg cccagatgtc ggagcgcggc gaacagctgt gcaaccagta
     1921 catcatcccg cgggtgctga agccgatctc ctcggcgatc gcgtcgcgat cgtaacgcgg
     1981 caacattttg ctccagcgcc cggtattcct tattgtttag cggccgcgtt tgcgcggcgc
     2041 taaactctga tcccctcgcc catgtactca tgggagaggg ttagggtgag ggtgatttgg
     2101 aaatttcgag cggctatcga aatctaaatc gccctcaccc tgaccctctc ccggagtacc
     2161 gggcgagggg accaggatga atacgcggcg ctgacggcgc gaagggcggc aaagggaaga
     2221 tccgtcgggc gaagggacca taaaaccatg ctctctgcag acgaacacga actgcttgtg
     2281 caggctcgcc agcggacgct ggctttgctg gagtcgttgg agcaaagtcg cgctcagctg
     2341 agcgacccga tgtggtcgat cgacgccgcc accgccgccg aggggcgcgt gtcatacacc
     2401 gccgtgattg acgccgctcg cggcacattg gacaatcttg accgggcgct gagtgggaac
     2461 gaggatcgca ccagcaacag cgccgcagcg gccaccgcga gtgagacgta aaacgatgcc
     2521 cgagggacca ccgccataca cggggggcgt gatcatcgcc tccgccgcca aagactatcg
     2581 cctgaagcgc tacgcgctgg tcggcatcct gctcatctac gggttgctca gcatcaaaga
     2641 cggtttctac cgctacccgc gcgagaacgc cgaagccgaa cagaaatacc ccggcaccaa
     2701 gcccccacat ccgggtttcg acgtgcagtt gaaccagggc cttggcatcc tgttgccgcc
     2761 gctgtcgatc gcgttcctca tctggtgctt gtacagctcg cgcgggcact atcgcttcga
     2821 cggccagacc ctcaccgtcc cgggacatcc gccggtgccg ctcaacgcgc tgcgacggat
     2881 cgaccgcgcc aagtgggacc
//
LOCUS       DATIMH010000714         8441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2190643, whole genome shotgun sequence.
ACCESSION   DATIMH010000714 DATIMH010000000
VERSION     DATIMH010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190643"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VLI90_12545"
     CDS             complement(<1..675)
                     /locus_tag="VLI90_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15079.1"
                     /translation="MGLFPRRQLTLLLLLALALRAGWGLVQPTDDAAIDRLPDQREYL
                     SLGRNLLHEHALAFVDPRFDETVYAYRTPGYPAFIALCGGSVRAIRSVQAILDTCTVL
                     AVFLLARQLSGNAGIALLAGAVVALNPFLIYFSGLILSETLFTAALAWGMVLLIRRMT
                     VAAGMVILLAVLVRPSALALAPILAITSIYANSSAGSAYRLLAGIRICLIVLTIIVLG
                     LFPWAYR"
     gene            complement(990..2525)
                     /locus_tag="VLI90_12550"
     CDS             complement(990..2525)
                     /locus_tag="VLI90_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15080.1"
                     /translation="MASRLGRRRRASLAVLAVVAGTSGAVFAADTTPASEPSAQLTQQ
                     QMLDQLKELQAEVKELKAAQAGKSTESKPATEAAAATAPSSDSASTLAAVQQDADRKT
                     GGQFSFSALSAVTGYDGKGFVLSDESGDNVFRPGYILQARNNTNWRQNGKNFGADSDT
                     ENGWEIRRAKIYLTGNLFTPDLTYRFQMANQVNGPSFVLDDAWMAYHFYTGGLGKLSF
                     KVGQFKIPVVHEEFEVGDEKPMAAERSLLNDTIGGGVMGPRSQGIDLLWNSPSDVLHG
                     EAMLHDGSGNSVGAGGTFRRGTANVVLPGNVAGSVNTDFRDGKTQGAPIWGAAARAEW
                     KATGSWDDYIDFTARASKKPLLVIGAGVDETEGVNTWYTHVSADLQYKHPCGASIYVA
                     GIGSFTNFRNIAGFNNNFDYGALVQVAYAINHKVEPFIRFDAIKLDDDVAPSNNLISE
                     FTVGVNYYMGEEGRYGHHAKFTLDASYLPIGSGPTDHLNTDELANSFHNEVLLQAQFQ
                     LWL"
     gene            complement(2782..4416)
                     /locus_tag="VLI90_12555"
     CDS             complement(2782..4416)
                     /locus_tag="VLI90_12555"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSV15081.1"
                     /translation="MNVLLRAMLPEIVLCAVACVLFLLGVSTRVLSRRLAGEIALVTL
                     VVVFLIQLFNTGHGADHHALIDDFGAVHIYSFSQYIKLIAAGVGALLVLLNMPTNAEA
                     TGGSASDFGTETGEFFGLLLLSLAGLFIVASANDIILLFLGIELASIPTYIMVSISRP
                     IPAAQEAGVKYFFLGAMSAAVMLFGFSYLYGTTGSTSLDVIASRFAEFSHPGRVDLTE
                     WQLLAVLMLIAGFAFKIAAVPLHVYAGDVYQGAATPVTAFLAFVPKTSGFVALIKVLL
                     AIGANTWAVPDTVVKLLWILAALTMTFGNVLGLLQFNVKRVLAYSSVAHTGYMLVGLT
                     ALVSTSDPNIQTESLQGVLFYLAAYGIMNAGAFGVLLLIPSRLNRPATSAETYEDLAG
                     QGRRHVGLGLAMAVACFSLTGLPLTIGFFGKLYLIKPALHAGLNGLVIVLVINAAISA
                     AYYLRIVASMFLRPEPTHQSTGGPAIEAPLRLVHPAPVVIGLWISVIATLAFGIFFPA
                     TRMLSQRTEQAAIFEPAPVLPTAPPAVAAASGDVRP"
     gene            complement(4482..6065)
                     /locus_tag="VLI90_12560"
     CDS             complement(4482..6065)
                     /locus_tag="VLI90_12560"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSV15082.1"
                     /translation="MLPLLLLLPVIGAIVAAVLPRARDAKFWALLVALLMGVIGVYLL
                     VAFPYAQRGWQFIFPAKDSDWLSLSSIGFSFSLGMDTISLWLVILTILLMPLSIAASF
                     DSIKDRPKQYYAWMLALSTAMLGVFVARDLLLFYIFFELTLIPMFFIIGIWGGPERRY
                     AAAKFFLFTFAGSVFTLAAVVYLGMKAGTFDIDAVTHFAQTSMSDTERFWVALGFLAG
                     FSVKVPLFPVHTWLPLAHTEAPTAGSVILAGVLLKLGTYGILRLVIPIGLVARNGFLF
                     PNLIACIGVLCLIGIIYGALVAWVQQDIKKLVAYSSVSHLGFCVLGLMALNDYGIQGS
                     VLYMVNHGLSTGALFLCIGMIYERYHTRDINELSGLARIMPKLAFFFVLFALSSIGLP
                     GLNGFTSEFLTILGAFTSNYLGIGFGSIAALGVILGAVYMLHMVARVIWGPLKVPGGH
                     GGDHGHGHEKHASTDINSREVAILVPLALAVVFLGVLPTSLMRRMLDPIQRLQQPITL
                     TTPTPEAKVPTPTVVAAAK"
     gene            complement(6186..8354)
                     /gene="nuoL"
                     /locus_tag="VLI90_12565"
     CDS             complement(6186..8354)
                     /gene="nuoL"
                     /locus_tag="VLI90_12565"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSV15083.1"
                     /translation="MNWVIDHSYLIPLLPMLGAIIAGFFGARWLRGQSHWPIWIGVGI
                     AAIMSITLLVGMLHEVAGAGEGAVPIGHIKDFYTWIEAGNFKVMAGFFFDPLTAVMLC
                     VVCGIGFFITVFSAGYMKGEEGYFRFFAYLGLFIFSMTCLVMGNNLVMLYLGWEGVGL
                     CSYLLIGYYYERPSAREAAKKAFLVNRIGDFGFGLGIMLSFYAFGTISYYGTGVGSNT
                     GLLEMAKNALLHSTDGNAPQQFATQLWALKYIPFLLMLGAFGKSAQFPLYVWLPDAME
                     GPTPVSALIHAATMVTAGVYMIARCGTLFVTNHAAMLTIASVGAFTALFAATIALRQF
                     DLKKVFAYSTISQLGYMFIGVGVLAPTAAVFHLVTHAFFKALLFLSSGVVMHAMLGHL
                     DMRKMSGLKKVLPITNVLMLIGCAALAGFPFLSGFFSKDDIVAAAYQRNVIIWAIALL
                     TAFITAYYTFRLYFRVFQGPLIVPTEAAEGHAHPAHDEAHAEAGASHAAVQTGTEQTS
                     GVDKAAAHSEEHGHHNHEPMLMILPLIVLAIGALFAGYLNVPWPKLSQFLGHSPSFQY
                     AYDLASARYAGDQPVSPEAFGVVERTAGETAFNIWPMVIGGLVSIAGISLAYLLHLRD
                     RARAERIAAGLEPVTALLEAKYWVDEIYQSAIVEPLRDIGRAFFWIDRVIVDGIVWAI
                     GFIPQLGGFALKLSVQRGYLQGYATAMLLGILAILLFVFL"
BASE COUNT         1707 a         2503 c         2811 g         1420 t
ORIGIN      
        1 tcgatacgcc caggggaaca gcccaagcac gatgatcgtc aggacgatca ggcaaatccg
       61 gatgcccgcc agcagtcggt acgccgagcc cgctgaggag ttcgcataga tgctggtgat
      121 cgccaggatc ggagcgagcg cgagcgcgga tggccgcacg agaacggcca gcaatatcac
      181 catgccggcg gcgacggtca ttcgtcgaat cagcaacacc atcccccatg ccagcgcggc
      241 ggtgaagagc gtctcgctca ggatgagtcc actgaagtaa atgaggaacg ggttgagcgc
      301 gacgacggca cccgccaaca aagcaatgcc ggcgttgccc gacagttgcc gggcaagcag
      361 gaacacggcc agaaccgtgc aggtgtcgag gattgcctgc accgaacgga tggcgcgcac
      421 cgatccgccg cacagcgcga tgaacgccgg ataacccggc gtacggtagg cgtacacggt
      481 ttcatcgaac cgcggatcga cgaacgccag cgcgtgctcg tgcagcaggt ttcgcccaag
      541 cgaaagatac tcgcgctggt ccggcagccg gtcgatggcc gcatcatccg ttggctgcac
      601 tagaccccag cccgcgcgaa gcgcgagggc gagcagaagc agcagcgtca gttggcggcg
      661 aggaaacaag cccatcgatc aaacggtgta acagaacgcg tcgcgccgca acagccgcgg
      721 ggtgtcgggc actgcccgtc acagggcgaa ggccagtggg cagtgcgcgc tccggtcccc
      781 tcgcccggta ctcctgacga accgacattt ttgcaggaat ggttgggagc cgaccacgcc
      841 gggttcggtc ccttgttaaa tctgcaaaag tgtcgggccg tcggcccagt actccggcac
      901 caatgcccgc gctccagcat caccggcaac gaaaaagggc ggccgcgatc ggccgcccgg
      961 gaacttgcgc tttcaaactt cgaaaatcct cacagccaca gctggaactg ggcctgcagc
     1021 agcacttcgt tatggaagct gttggccagt tcgtcggtgt tcaggtggtc cgtggggccg
     1081 gagccgatgg ggagatagct ggcgtccagc gtgaacttgg cgtggtggcc gtagcggcct
     1141 tcttcgccca tgtagtagtt gacgccgacc gtgaactcgc tgattaagtt attgctgggg
     1201 gcgacgtcgt cgtccagctt gatggcgtcg aagcggatga agggttcgac cttgtggttg
     1261 atcgcgtagg cgacctgcac cagcgcgccg tagtcgaagt tgttattgaa gccggcgatg
     1321 tttcggaaat tggtgaagct gccgatgccg gcgacgtaga tgctggcgcc gcaggggtgc
     1381 ttgtactgca ggtcggcgct aacgtgggtg taccacgtgt tgacgccttc ggtttcatca
     1441 acgccggcgc cgatgacgag cagcggcttc ttactggcgc gggcggtgaa gtcgatgtag
     1501 tcatcccaac tgccggtggc cttccattcg gcgcgagcgg cggcgcccca gatgggcgcg
     1561 ccctgggttt tgccatcgcg gaagtcggtg ttgaccgaac cggcgacgtt gccgggcagg
     1621 accacgttgg ccgtgccgcg gcggaaggtg ccgcccgcgc cgacgctgtt accgctgcca
     1681 tcgtgcagca tcgcttcacc gtgcagcacg tcgctggggc tgttccagag caggtcgatc
     1741 ccctgcgagc gcggccccat cacgccgccg ccgatcgtgt cgttcagcag cgagcgttcg
     1801 gcggccatcg gcttttcgtc gccaacttcg aattcctcgt gaacgaccgg tatcttgaac
     1861 tgaccgacct tgaatgacag cttcccgagg ccaccggtgt agaagtggta cgccatccac
     1921 gcatcgtcca gcacaaagct ggggccgttc acctggttgg ccatctggaa gcgataggtg
     1981 agatccggcg tgaacaggtt gccggtgaga tagatcttgg cgcggcggat ttcccagccg
     2041 ttttcggtgt cgctgtcggc gccgaagttc ttgccgttct gtcgccagtt ggtgttgttg
     2101 cgggcttgaa ggatgtaacc ggggcggaag acgttgtcgc cggattcatc gctgagaacg
     2161 aagcctttgc cgtcgtaacc cgtgacggcc gacagggcgg aaaacgaaaa ctgtccgcca
     2221 gttttgcggt cggcatcctg ctgcaccgcg gcgagggtgg acgccgagtc cgatgacggc
     2281 gcggtcgcgg cggccgcttc ggttgcgggt ttggattcag tgctcttccc cgcctgggcg
     2341 gctttcagct ccttcacttc cgcttgcagc tccttgagct gatcgagcat ctgctgctgc
     2401 gtgagctgcg ccgaaggctc cgacgccggc gtggtgtcgg cggcaaagac cgccccggaa
     2461 gtccccgcca caaccgctaa aacggccaac gatgccctgc gtcgccggcc caatctcgat
     2521 gccatatacc tgctcctctt gtcgtaaagg tcgattccta aaccttgcga tcggcgagat
     2581 ggatcgtttt tcagtagtgg cgggcgtagc cgataatgta atcgaccgcg tcggcgcggg
     2641 aatatcccgg tcccgcaagc ttcacctgat cccccggcgg tgtcgccggg ggacgcggga
     2701 aatgctaagg cgcagcgata acttcggatt aaggcggcga gtgggatgtg caaagatcat
     2761 taaggcgcgg cgatatgtag actacggccg gacatcgccc gacgcggcgg caacggctgg
     2821 gggcgcggtg ggaagcaccg gcgcgggttc gaagatcgcg gcttgctcgg tgcgctggct
     2881 gagcatccgc gtcgccggaa agaagattcc gaaggcgagc gtggcgatga cggagatcca
     2941 caggccgatg acgaccggcg cggggtggac gaggcgcagc ggggcctcga tcgcggggcc
     3001 gccggtggat tgatgcgtcg gttcggggcg gaggaacatc gaggcgacga tgcgcaggta
     3061 atacgccgcg gagatggcgg cgttgatcac gaggacgatc acgagtccgt tcaagcccgc
     3121 gtgcagcgcg ggcttgatca ggtacagctt accgaagaag ccgatcgtga gcggaagacc
     3181 ggtgaggctg aagcacgcga cggccatcgc gagcccgagt ccgacgtggc ggcggccttg
     3241 gccggcgagg tcttcgtagg tctcggcgct ggtcgctggg cgattgaggc ggctggggat
     3301 cagcagcagc acgccgaacg ccccggcgtt catgatgccg taggcggcga gatagaacag
     3361 cacgccttgc agcgattcgg tttggatgtt cggatccgaa gtgctgacca gcgcggtgag
     3421 acccaccagc atgtaaccgg tgtgagcgac ggagctgtac gccagcacgc gcttgacgtt
     3481 gaactgcagc aggccgagga cgttgccgaa cgtcatcgtc agcgcggcga gaatccacag
     3541 cagcttcacc accgtgtcgg gcacggccca ggtgttggcg ccgatggcga gcaggacctt
     3601 gatcagcgcg acgaacccgc tggtcttggg gacgaaggcg aggaacgccg tcaccggcgt
     3661 ggcagcgcct tggtagacgt cgccggcgta cacgtgcagc gggacggcgg cgatcttgaa
     3721 cgcgaagccg gcgatcaaca tcagcaccgc cagcagttgc cattcggtga gatcgacccg
     3781 gccggggtgg gagaactcgg cgaagcgcga cgcgatcaca tccaggctgg tcgagccggt
     3841 cgtgccgtag agatagctga agccgaagag catgaccgcg gcactcatcg cgccgaggaa
     3901 gaagtatttg acgcccgcct cctgggcggc ggggatgggc cggctgatgc tgaccatgat
     3961 gtaagtcggg atgctggcca gttcgatgcc gaggaacagc aggatgatgt cgttggcgct
     4021 ggcgacgatg aacagcccgg cgaggctgag cagcagaagt ccgaaaaatt cgccagtctc
     4081 agtgccaaag tccgaagcgc tgccgccggt cgcctcggcg ttggtcggca tgttcagcag
     4141 cacgagcaac gccccgacgc cggcggcgat cagcttgatg tattggctga agctgtaaat
     4201 gtgaacggcg ccgaaatcgt cgatcagcgc atgatgatca gcgccgtggc cggtgttgaa
     4261 cagctggatg agaaagacga cgacgagcgt gaccagcgcg atttcgccgg cgaggcggcg
     4321 cgagagcacg cgcgtggaaa cgcccagcag gaacagcacg cacgcgacgg cgcacaggac
     4381 gatctcgggc aacatcgctc ggagcaaaac gttcatatta gtagcacggg catctcgccc
     4441 gtgtctgagc acctaggcac gggcgagacg cccgtgctac tttatttcgc cgcggccacc
     4501 actgttggcg ttggcacctt cgcttctggc gtcggcgtcg tcagcgtgat cggttgctgc
     4561 aatcgttgga tcgggtccag cattcgtcgc atcagcgacg tcggtaatac gccgaggaag
     4621 acgaccgcta gcgccagggg gaccaggatc gccacctcgc ggctgttaat gtcagttgac
     4681 gcatgtttct catggccgtg cccatgatcg ccaccatgtc cacccggcac cttcagcggt
     4741 ccccagatca cgcgggcgac catgtgcagc atgtagaccg cgcccaggat cacgcccagg
     4801 gcggcgattg aaccgaaacc gatgccgaga tagttactgg taaatgcgcc gaggatcgtc
     4861 aggaattcgc tggtgaatcc gttcaggccg ggcaggccga tgctgctgag cgcgaacagc
     4921 acgaagaaga acgcgagctt gggcataatg cgggccaggc ccgacagttc gttgatgtcg
     4981 cgcgtgtgat agcgctcgta gatcatgccg atgcacaaga acagtgcgcc ggtgctcagg
     5041 ccgtggttga ccatgtacag gaccgagccc tgaatgccgt agtcgttcag cgccatcagc
     5101 ccgagcacgc agaagcccag gtggctgacg gaggaatagg cgaccagctt cttgatgtcc
     5161 tgctgcaccc aggcgaccag cgcgccgtag atgatgccga tcaggcaaag cacgccgatg
     5221 cacgcgatga ggttggggaa caggaagccg ttgcgcgcga cgagtccgat cgggatgacc
     5281 agccgaagga tgccgtacgt gcccagcttc agcagcacgc cggccaggat gaccgagccc
     5341 gcggtgggcg cttcggtatg cgccagcggc aaccaggtgt gcacggggaa cagcggcacc
     5401 ttcacgctga aaccggcgag gaagccgagc gcgacccaga agcgttcggt gtcgctcatc
     5461 gaggtctgcg cgaaatgcgt gaccgcgtcg atgtcgaacg tgccggcctt catcccgaga
     5521 tacacgacgg ccgccagcgt gaagaccgag ccggcgaagg tgaacaggaa gaatttcgcc
     5581 gccgcgtaac gccgttcggg gccgccccag atgccgatga tgaagaacat cggaatgagc
     5641 gtgagttcga agaagatgta aaacagcagc aggtcgcggg cgacgaacac gccgagcatg
     5701 gcggtgctca gcgccagcat ccaggcgtaa tactgcttag ggcggtcttt gatcgaatca
     5761 aagctcgccg cgatcgacag cggcatcagc aggatggtga ggatgaccag ccaaaggctg
     5821 atcgtatcca tgccgaggct gaaactgaaa ccgatgctgg agaggctgag ccagtcgcta
     5881 tctttggcgg gaaagatgaa ctgccagccg cgttgcgcat acggaaacgc gacgagcaga
     5941 tagacgccga tcacgcccat gagcagcgcg accagcaacg cccagaactt agcgtcgcgc
     6001 gctcgcggca acaccgcggc gacgatcgcg ccaatgacgg gcaggagcag gaggagtggc
     6061 agcacgtatt gggacataag attttaacca cagatgcaca cagatgaacc cagataagag
     6121 caggcgagcg aatcagcctg atctgtgtgc atctgtgtgc atctgtggtt ccatctatct
     6181 ttcacttaca gaaacacgaa cagcaaaatc gccaagatcc ccagcagcat cgccgtggcg
     6241 tatccctgta aatatcctcg ctgcacgctg agtttcaggg cgaacccgcc cagttggggg
     6301 atgaagccga tggcccagac gatgccatcg acgatcacgc ggtcgatcca gaagaacgcc
     6361 cggccgatgt cgcgcagggg ttcgacgatc gcgctctggt aaatctcatc cacccaatac
     6421 ttcgcttcga gcaacgccgt caccggctcc agcccggcgg cgatgcgctc ggctcgggcg
     6481 cggtcgcgca ggtgcagcag gtacgccagt gagatgccgg cgatcgagac caggccgccg
     6541 atgaccatcg gccagatgtt aaacgccgtc tcgcccgcgg tgcgctcgac gacgccgaag
     6601 gcttcggggc tcaccggttg gtcgccggcg tatcgcgcgg aggccaagtc atatgcgtat
     6661 tggaacgacg ggctgtgccc gaggaactgc gacagcttcg gccacgggac gttcagatat
     6721 cccgcgaaga gcgcgccgat cgccagcaca atcagcggga ggatcatgag cattggctcg
     6781 tggttgtggt ggccgtgctc ctcggaatgg gcggcggctt tgtcgacgcc ggaggtttgc
     6841 tcggtgccgg tctgcaccgc ggcgtgactg gcgccggcct cggcgtgggc ttcatcgtgg
     6901 gccggatgcg cgtgcccttc ggcggcttca gtcggcacga tcagcgggcc ttgaaacacg
     6961 cggaaataca ggcggaacgt gtagtacgcc gtgatgaacg ccgtcagcag cgcgatggcc
     7021 cagatgatga cgtttcgctg atacgccgcc gcgacgatgt cgtctttgga aaagaacccg
     7081 ctcaggaacg ggaacccggc cagcgcggcg cagccgatca gcatgaggac gttcgtgatc
     7141 ggcagcactt ttttcaggcc ggacattttc cgcatgtcca ggtggccaag catcgcgtgc
     7201 atgacgacgc cggaactgag gaacagcaac gctttgaaga acgcgtgggt gaccaggtgg
     7261 aacaccgcgg ctgtcggcgc caagacgccg acgccgatga acatgtaacc caactggctg
     7321 atcgtgctgt aggcgaagac tttcttcagg tcgaactgcc gcagcgcgat ggtggcggcg
     7381 aacaatgcgg tgaacgcgcc gacgctggcg atcgtcagca tggccgcgtg gttggtgacg
     7441 aacagcgtgc cgcagcgggc gatcatgtaa acgccagccg tcaccatggt ggcggcgtgg
     7501 atcagcgcgc tgaccggcgt cgggccttcc atcgcatccg gcagccagac gtacagcggg
     7561 aactgtgccg acttaccaaa ggcgccgagc atcaacagga acgggatata tttcagcgcc
     7621 cacagctgcg tggcgaactg ctgcggggcg ttaccgtcgg tcgaatgcag cagcgcgttc
     7681 ttcgccatct cgagcaatcc agtattgctg cccacgccgg tgccgtaata gctgatcgtg
     7741 ccgaaggcgt agaagctgag catgatgccc agaccgaagc cgaagtcgcc gatgcggttg
     7801 acgagaaacg ctttcttcgc cgcctcgcgc gcgctcggcc gctcgtagta atagccgatc
     7861 agcaggtacg agcagaggcc caccccttcc caacccaggt acagcatcac caggttgttg
     7921 cccatcacca ggcaggtcat cgagaagatg aacaggccga ggtaggcgaa gaagcggaaa
     7981 taaccttcct cgcccttcat gtacccagcg gaaaaaacgg tgatgaagaa gccgatgccg
     8041 cagaccacgc agagcatgac cgcggtgagc gggtcgaaga agaaccccgc catcaccttg
     8101 aagttgccgg cttcgatcca ggtataaaaa tctttgatgt ggccgatcgg caccgcacct
     8161 tcacccgcgc cggcgacctc gtgcagcatg ccgaccagca gcgtgatcga catgatcgcg
     8221 gcaatgccga cgccgatcca gatgggccaa tgcgattggc ccctcagcca tcgcgccccg
     8281 aaaaacccgg cgatgatcgc gccgagcatc ggcagcagcg ggatcagata ggagtggtcg
     8341 atgacccagt tcatttcgta ttccgcacag cgctttctgg tcccctcgcc cggtactccg
     8401 ggagagggtt agggtgaggg tgatttggga tttcgaaagc c
//
LOCUS       DATIMH010000715        10328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1617407, whole genome shotgun sequence.
ACCESSION   DATIMH010000715 DATIMH010000000
VERSION     DATIMH010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10328
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617407"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VLI90_12570"
     CDS             <1..955
                     /locus_tag="VLI90_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15084.1"
                     /translation="AIATESNGVTVEPWDLSTGQKTTGEPLKLKTRPASKFAISTDGK
                     RIAWVATFPKHSVQVWSFDTGKATMAIDWEAARGDPELIGFSAPNRLLLRWGNTSASA
                     DIDVVNVDAKARVATFKVNQLQGKHPIAVSPAAQRLLVASKVKGIPTLIQYDLNDGQP
                     ILYTKIVDLDPALPVDARGLAYSNDGKRMAIYIENPEGALLVSYDSDGSQKPVSLVYP
                     DGPTPGVAHDNFADNALTSLDPSPCWLIYGDAVIDAKTNDVLANLGVKNVIANRVLDN
                     DHVELVTSDGSKVQVKVATIDRSKLQALTATPTPAPQVSLH"
     gene            1802..3841
                     /locus_tag="VLI90_12575"
     CDS             1802..3841
                     /locus_tag="VLI90_12575"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HSV15085.1"
                     /translation="MSHSRIRYGENDLVQQLNTAADSVMEPLEARILFAADIRTLDGT
                     GNNLAHLTWGSTNVDLLRLAPAQYGDGISSPAGANRPSARVISNAIAAHPADTEILSA
                     NHLSAFAYLWGQFIDHDLDLTTTSASAGEFDVPVPSGDPQFDPAGTGTQVIPLTRSTY
                     DPATGATTPRQQINVITAFLDASMVYGSDAARAAALRAFVGGRLLTSAGGLLPFNTNG
                     LANANDAHIFPDNQLFLAGDVRANENSELTSLQTLFVREHNRLAGNIAAQHPNWSDEQ
                     LFQEARRLVIGEIQSITYNEFLPALLGQNALGRYTGYNPSVNAGISTEFSTAAYRLGH
                     SMLTDDVEFINNDGTDARAAMSLSQVFFNPSVVESTGIDPIVKYLASSNSEEIDNKVV
                     DSVRNFLFGPPGAGGLDLASLNIQRGRDHGLADYNTTRASLGLPRITGFGQITSDRVL
                     AGKLQSLYGSVDNIDLWVGGLAEDHAPGSNVGPTFQRILVDQFTRTRSGDRFWYQRDL
                     SPAELSMVNSITLADVIAVNTGISNLQPNVFVFDVHVTGQIYYDRNTAGHIGRGETGV
                     GNMTVNLVASDGTIVNTTITSRDGHYRLSNIQLGDYTVVPVLPSGAWYTTATPVTINV
                     TRGGWFTNVDFGVGGLSQQNPPHQQPVWSWGNGNLPSSSWLDLQSFHPRWKDLLV"
     gene            3884..5161
                     /locus_tag="VLI90_12580"
     CDS             3884..5161
                     /locus_tag="VLI90_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSV15086.1"
                     /translation="MPTTSTRRAFLQSAAAAGVSASLVRAGDSAPATAPTSPSVTKAE
                     LDHILDQPVLKTDFLSSPVMVASIEVLRNGRNFLLRTRSTDGIVAITVPNSDRLANVY
                     PLLINQIIPVFLNKDARTLESLLWDVYRYKDNYKFYGLALWVGVAAVEMALLELMGQT
                     TKRPLADFFGGAVRRDIPIYVASGVRGNTPEAEIDHLRQLVTDSGAKALKFRLGGRMS
                     RNADSLPGRTEALIPLVRKTFGDDFTLYADANSSYDVPNALRIGRLMEEHRYGFYEEP
                     VPFDELWETKEVADALTIPIALGEQEASFRRFQFCIQNRVADIIQPDLHYFGGFIRST
                     KVARMCAAAGINLVPHMSGGSLGYLDVVHFASITPNIGPYMEFKGNTNLPVHCDTSPV
                     KSVDGVVRCPSGPGFGVTIDPGYVAKAKPVDLI"
     gene            complement(5297..6301)
                     /locus_tag="VLI90_12585"
     CDS             complement(5297..6301)
                     /locus_tag="VLI90_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15087.1"
                     /translation="MRTSLTLLIALFLFSLPARAEWPARVFAPYMYIGQGDNFKLTDC
                     DDACGLKHYTLAFIIAKQEGTKKEAKYFKESSWDGRFPIDRSVYGDQIEAIRKRGGDV
                     IMSFGGEAGKELANVIDDPAELEAAYQKVIDTYKFTWLDFDVEGNNLDRGKRDSERRN
                     TVLAQLQKKNPELIISYTLPVDPMGLSDASQDLLTDAVRQGVKVHSANIMVMYFGKQF
                     IHKGRGEGELGVDSANAAYAQLQKIDPSIKVGLCPCLGNNGSKDEVFDLEDAKTIKAF
                     ADQTPWVCSLHYWSINDDGAKPRKKRATTKPSTQAATPAPPQQPWAFANIFKSFTTAQ
                     "
     gene            complement(6583..7878)
                     /locus_tag="VLI90_12590"
     CDS             complement(6583..7878)
                     /locus_tag="VLI90_12590"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSV15088.1"
                     /translation="MSSKRRPWQEELAIIDRTMKAISDVTDPEELVHIYWEGIGELLP
                     IRDYLSLSRRELRSPEYLITRGSRFTEHFNPWTQRALLPRFSGGLLAEIIYANKPVFI
                     DDLPARLADDEPARFYLDGYASLIALPQYDGGESLNVTISLFKSGDELDRSIIPMLHW
                     QSGLFGRGTTNLVLRNDLAKALATLDKELQVVGDIQRSLLPRALPEIPGCEVAAFYQT
                     SARAGGDYYDFFPLHHGGWGVFIADVSGHGTPAAVLMAITHAIAHAHPGTHTPPIELL
                     KYLNTHLTRSYTSGGTFVTAFYAVFDPASRSLTYSSAGHNPPQLVRGDRVISLDQNGA
                     LPLGIEQEQAYHQTTLTLQKGDLLLLYTDGITEAMAPSIGGAPRELFGVERLDRVLLD
                     CGAASAEACVARIRNELLAFCAQLPQTDDQTLIAIRCLP"
     gene            complement(7956..8438)
                     /locus_tag="VLI90_12595"
     CDS             complement(7956..8438)
                     /locus_tag="VLI90_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HSV15089.1"
                     /translation="MNLIVRESPTGRGVFASTHLSAGARLLRFTGPLLRYEQTTPQTL
                     ALQIGLDLYIGESGGADDCVNHSCDPDAGLVIDGTDVQLIALRDIAAGEQVTFDYSTT
                     MDEDDFEFDCLCGSPNCRRRIRDFKHLPAEVQRRYVELGVVPEYNRRYVEAAPSPTWR
                     "
     gene            complement(8545..9036)
                     /locus_tag="VLI90_12600"
     CDS             complement(8545..9036)
                     /locus_tag="VLI90_12600"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV15090.1"
                     /translation="MSFNRVEPPDHLLCQTDLGVVKIRRATLADIADLRHRVLRVGLP
                     RESAIFTGDDLPGTRHFAAFYTDRAVSCASFHENPWQAEPAWQLRGMATDDAFRSKGV
                     GRALLAFAESSLRQERSDIHRLWANARVPAARFYQSLGWAIASEMFDIPTAGPHFRMT
                     KLF"
     gene            9149..10033
                     /locus_tag="VLI90_12605"
     CDS             9149..10033
                     /locus_tag="VLI90_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15091.1"
                     /translation="MPIVVACNCGKRFKAGDQFAGKRTKCPGCGQALTIPGGAPAVAT
                     AAPQKSAAPKAVAAAAPPARRTPVAAARSAPVRAAPPPPLPAFEDDLSTLGEPEEVFQ
                     PAARSENDAPVKTVKRGTSALAAAAAVAPPGKRKAGPASAGAPRFSMSPGMIVLVVLA
                     ILIPSTITWVKLGPMKAAEEWNKISSIAEDNISGQIARSLLSQRAKLGFDVRDVKYMP
                     KTTNFQFDESKAFMMIKMPETVPFQGKTTDGNFYGTFHPKTMQFEADVEMMDGKHKLT
                     GSASESDTSLAIDGTAVK"
BASE COUNT         1983 a         3401 c         3079 g         1865 t
ORIGIN      
        1 cgccatcgcg accgaaagca acggcgtcac cgtcgaacct tgggatttat ccaccggcca
       61 aaagacgacc ggtgaaccgc tgaagctcaa gaccagaccc gcgagcaagt tcgccatcag
      121 caccgatggc aagcgcatcg cctgggtcgc aaccttcccg aagcattcgg tgcaggtctg
      181 gtccttcgac accggcaagg cgacgatggc catcgactgg gaagccgccc ggggtgatcc
      241 ggaactgatc ggcttctccg cgcccaatcg cctgctgctt cgctggggca acaccagcgc
      301 atcggcggac atcgacgttg tgaacgtcga tgccaaggcg cgcgtcgcca cgttcaaggt
      361 gaaccagctg cagggcaagc acccgatcgc cgtcagcccg gccgctcaac ggctgctcgt
      421 cgccagcaag gtgaagggaa tccccacgct catccaatat gacctgaacg acggccagcc
      481 gatcctctac acgaagatcg tcgatctcga tcccgccctc cccgtggacg cccgcggcct
      541 cgcctactcc aacgacggca agcgcatggc catctacatc gagaaccccg aaggcgcgct
      601 gctcgtctcc tacgacagcg atggctccca aaagcccgtc tcgctggtct accccgacgg
      661 tcccacgccc ggcgttgcgc acgataactt cgccgacaat gccctgactt cgctcgatcc
      721 atccccgtgc tggttgatct acggagacgc cgtcatcgat gccaagacca acgacgtctt
      781 agcaaacctc ggcgtcaaaa acgtgatcgc caaccgagtg ctcgacaacg accacgtcga
      841 actggtcacc agcgacgggt caaaggtcca ggtcaaagtc gccaccatcg accgcagcaa
      901 gctacaggcg ctgaccgcca cgccaacgcc ggcgccgcag gtgtcgctgc actgaagcgc
      961 tttacacccg acgtagtggc gcgcggtcat gctccagcgc agaggtactc gccggagtcg
     1021 aaggatctgg ccaaggcgtc gatgaaccgc tcccgcgcga gcagcgccgc acagccgctt
     1081 gcagctcagc atctgttgcg aatttccaaa acatgctaag ccgcaagcgg cagctttcgc
     1141 gcaagcggcg cctcacttac ttcgcggctg gatccttcga acccaaatga cgaggaatcg
     1201 cagcatccac ccctcccccg acaatttcac cacatttttg aaaccaccgc cgccgccacg
     1261 tccactaatg ggctggacgg aagggtaata ttttttcgtt gccagccgga attttgtggc
     1321 gtattcggca acgaagtgca accttatgta gctgacccca aacgagtgac tgcgcttgct
     1381 gcgcagcggg tcagcgaggg ttgtggacga ttcagtcaac aaaaacagcg ggccgcccgc
     1441 gcgtgcctga cgagcacgcc gacgcccagt tcgaagctgt gcgcctttgc gcgcagtgtc
     1501 acctccgaac ttccggcggg cccgctgggt gtaatggtga tgccatcgcc ggcgtgggat
     1561 tacgtttgtt gaaagggcgg gcacttctgc cggcgaacat gcggtgcagc gcgcggacgc
     1621 gcggaactgc accctttctg tggcatcaac cacaccccac gcgctctcca aaatcgattt
     1681 tcgcgaaccc caacgcagac gaacccgcgg cgcgtatttc ttgcgcgagc gttcgaagcc
     1741 agcctttggc cgcccgcgcg cgcaagcttg gatgccgccg ctctttcaga ggaggtacac
     1801 catgtcccac agcaggatac gctatggcga gaatgatctt gtgcagcagc tgaacaccgc
     1861 cgccgactca gtgatggaac cgctcgaagc ccgcatcctc ttcgccgccg acatccgcac
     1921 attggacggc accggcaaca accttgctca cctcacgtgg ggatcgacga acgtcgatct
     1981 actgcgcctc gcccccgctc aatatggcga tggcatctcc tcccccgccg gcgcgaatcg
     2041 cccatccgcg cgcgtcatca gcaacgccat tgccgcccat cccgccgaca ctgaaatcct
     2101 tagcgccaac cacctgtcgg cgttcgccta tctctgggga cagttcatcg accacgatct
     2161 cgatttgacc accacgtccg catccgccgg cgaatttgat gttccggtgc cgagcggcga
     2221 tccgcagttc gatcccgccg gcaccggcac acaggtcatc ccgctgacga gatccaccta
     2281 cgacccggcc accggcgcca caacgccgcg gcagcaaatc aatgtcatca ccgcgttcct
     2341 cgatgcctcg atggtgtatg gctccgacgc cgctcgcgcc gcggcactac gcgccttcgt
     2401 cggcggccgg ttgctcacca gcgccggcgg gctattgccg ttcaatacca atggtctggc
     2461 caacgcgaac gatgcccaca ttttcccaga caatcagctg ttcctcgccg gcgacgtgcg
     2521 tgctaacgaa aacagcgagc tgacgtcgct gcagacgctc ttcgtccgcg agcacaatcg
     2581 actggcggga aacatcgccg cccaacatcc caattggtcc gatgaacagc ttttccagga
     2641 agcgcgccgc ctcgtgatcg gtgaaatcca atccatcacc tacaacgagt ttctgcccgc
     2701 cctgctggga caaaacgcgc tcggccggta caccggttac aaccccagcg tcaacgccgg
     2761 catctccacc gaattctcca ctgccgcata tcgcctcggc cacagcatgc tcacggacga
     2821 tgtggagttc atcaacaacg acggcaccga tgcccgcgcc gccatgtcgc tgtcgcaagt
     2881 cttcttcaac ccgtccgtcg tcgagtccac cggcatcgat ccgatcgtca aatatctggc
     2941 cagcagcaac tcagaagaga tcgataacaa ggtggtggac agcgttcgca acttcctctt
     3001 cggccccccc ggcgccgggg ggttggacct ggcgtcgctc aacatccaac gcggccgcga
     3061 ccacggcctg gccgactaca acaccacccg cgcctcgctc ggcctgccgc gcatcaccgg
     3121 cttcggtcag attacctccg accgcgtgct ggccggcaag ttgcaatcgc tctacggcag
     3181 cgtggacaac atcgacctct gggtcggcgg cctggccgaa gatcacgcgc ccggctccaa
     3241 cgtcggcccg acattccagc gcatcctggt cgatcaattc acgcgcacgc gcagcggcga
     3301 tcggttctgg tatcagcgcg acctgagccc ggccgagttg tcgatggtga acagcatcac
     3361 gctcgccgac gtcatcgccg tcaacaccgg catctcgaac ctgcagccca acgtgttcgt
     3421 cttcgacgtg catgtcaccg gccagattta ctacgaccgc aacaccgccg gtcacattgg
     3481 ccgcggtgaa accggcgtgg ggaacatgac cgtcaatctc gtcgccagcg acggcaccat
     3541 cgtcaacacg acgatcacca gccgcgacgg ccactatcgc ctgagcaaca tccaactggg
     3601 cgactacaca gttgtcccgg tgctgcccag cggcgcgtgg tacaccaccg ccacccccgt
     3661 cacgatcaac gtcacccgcg gcggttggtt caccaacgtg gacttcggcg tgggcggctt
     3721 gagccaacag aacccgccgc accagcaacc ggtctggagc tggggcaacg ggaatctgcc
     3781 atcaagttcg tggctcgatc ttcaatcgtt ccacccgagg tggaaggacc tgctcgtgta
     3841 atccaaaacc ggcggttcga cggcagtcgg ttatgatgcg gcgatgccaa ccacatcgac
     3901 acgccgagcg tttctgcagt cagccgcggc cgccggggtt tcggcttcat tggtccgcgc
     3961 tggggattct gcgccggcga ctgcgccgac ttcgcccagc gtgaccaagg cggagttgga
     4021 tcacatcctc gatcagccgg tgctgaagac ggattttctc tcgtcgccgg tgatggtcgc
     4081 gtcgatcgag gtcttgcgca atggccggaa ctttttgctg cgcacgcgct cgaccgacgg
     4141 cattgtggcg atcaccgtgc ctaattctga tcggctggcg aacgtctacc cgctgctgat
     4201 caaccagatc atcccggtct ttttgaacaa agacgcgcgc acgctcgagt cgctgctgtg
     4261 ggacgtgtat cgctacaagg acaactacaa gttctatggc ttggccctgt gggtcggcgt
     4321 ggcggccgtg gagatggcgc tgctggagtt gatgggacaa accacgaagc gcccgctggc
     4381 ggatttcttt ggcggcgcgg tgcggcggga cattccgatc tacgtcgcca gcggggtgcg
     4441 cggcaacacg ccggaggcgg agatcgatca tctgcggcag ctggttaccg actcgggcgc
     4501 gaaggcgctg aagttccgat tgggcggccg catgagccga aatgctgact cgcttccggg
     4561 gcgaaccgaa gcgctgatcc cgctcgtccg caaaacgttt ggtgacgact tcacgctcta
     4621 cgcggacgcc aacagttcat acgacgtgcc caacgcgctg cgcatcgggc ggttgatgga
     4681 agaacaccga tacggttttt acgaggaacc cgtgccattc gacgagttgt gggagactaa
     4741 agaggtcgcg gatgcgctga cgattccgat cgcgcttggc gagcaggaag ccagcttccg
     4801 gcggttccag ttctgcatcc agaaccgcgt cgccgacatc attcagcccg accttcatta
     4861 cttcggcggc ttcattcgct cgacgaaggt cgcccgcatg tgtgccgcgg cggggatcaa
     4921 tctcgtgccg cacatgtcgg gcggaagcct tgggtatctc gatgtggttc acttcgcgtc
     4981 gatcacgccg aacatcgggc cgtacatgga attcaagggg aacacgaacc tgccggtgca
     5041 ttgcgacacg tcgccagtca agagcgtgga tggcgtggtg cggtgcccct cgggcccggg
     5101 gtttggcgtg acgatcgatc cgggttacgt ggcgaaggcg aaaccggtcg atttgattta
     5161 acagcgcgcc gacgatgcgc acgaaccgcc tgatagccgc aggcgacagc cagcgggggc
     5221 tctcgaagtc agagcccttc gacttcccgc gcccccgctg gctgtcgcct gcggctgtga
     5281 aagatcaccg cgatcttcat tgcgctgtcg tgaacgattt gaagatgttc gcaaacgccc
     5341 agggctgttg cggtggcgca ggcgtggccg cttgtgtcga cggcttcgtc gtcgcgcgct
     5401 tcttccgcgg cttggccccg tcgtcgttga tcgaccagta gtgcagcgag cacacccacg
     5461 gcgtctggtc cgcgaaggct ttaatcgtct tcgcgtcttc cagatcgaac acctcatcct
     5521 tcgacccgtt gttgcccaga cacgggcaca ggccaacctt gatcgacggg tcgatcttct
     5581 gcagttgggc atacgcggcg ttggcggaat cgacgccaag ttcgccttcg ccgcggccct
     5641 tgtggatgaa ctgcttgcca aagtacatca ccattatgtt ggccgagtgc acctttacgc
     5701 cctgccgcac cgcgtcggtg agcaggtcct gcgatgcgtc ggacaatccc atgggatcga
     5761 ccggcagcgt gtaggagatg atcagctccg gattcttctt ctgcagctgc gccagcaccg
     5821 tgtttcggcg ttcgctgtca cgcttgcctc tgtcgaggtt gttgccttcg acgtcgaaat
     5881 caagccaggt gaacttgtag gtatcgatga ctttctgata cgccgcctca agctcagccg
     5941 gatcgtcgat gacgttggcc agctctttgc cggcttcacc gccgaatgac atgatcacat
     6001 caccgccgcg ttttcgaatg gcttcaatct gatcgccata cacgctgcgg tcgatgggga
     6061 agcggccgtc ccacgacgac tccttgaaat acttcgcctc cttcttggtc ccctcctgct
     6121 tggcaatgat gaacgccagc gtgtagtgct ttaggccgca ggcatcgtcg cagtcggtga
     6181 gcttaaagtt gtcgccctgg ccgatgtaca tgtacggggc gaagacgcgc gcgggccact
     6241 ccgcccgagc gggcagggag aacaggaaca gagcgataag aagggtcaga gatgtgcgca
     6301 tgcggtggtt ctcctgggca gtgattcgag caggaatatg cggactttgg aagcgggcac
     6361 gcgctgtcat cctgagcgca ggcggtactc cgcgaaggat ctaccccaga agtgactgaa
     6421 cgatcagtcg cgcgattggc gcggcagtcg cttgcgtctt cgcgagcccg cgacgacgcc
     6481 agcgactgcg atgcgttttt cgcgagccag cattcatcga gttttcggca agatccttcg
     6541 aatgcgcacg agttcgtgct tcgctcagga tgacggccgg ccctaaggca aacaacgaat
     6601 cgcgatcagc gtttggtcgt ccgtctgcgg cagctgcgcg cagaacgcca gcagctcgtt
     6661 gcgaatcctc gcgacgcacg cctcggcgct ggcggcgccg caatcgagca gcacgcggtc
     6721 gagccgttcg acgccgaata attcgcgcgg cgcgccgcca atggacggcg ccatcgcttc
     6781 ggtgatgccg tcggtgtaca gcagcagcag gtcgcccttc tgcaacgtga gcgtcgtctg
     6841 gtgatacgcc tgctcctgct cgatgccgag cggcagcgcg ccgttttggt cgagcgagat
     6901 gacgcgatcg ccgcgcacca gttgcggcgg gttgtggccg gcgctggaat acgtgagcga
     6961 tcggctcgct ggatcgaaca cggcataaaa cgccgtgacg aacgttccgc cgctggtgta
     7021 cgagcgtgtg aggtgcgtgt tgagatattt cagcaactcg atcggtggcg tgtgcgtacc
     7081 gggatgggcg tgggcgatcg cgtgcgtgat ggccatcaac accgcggccg gcgtgccatg
     7141 accggagacg tcggcgatga acacgcccca cccgccgtgg tgcagcggga agaaatcgta
     7201 gtaatcgcca cccgcgcggg cgctggtctg atagaacgcc gccacttcgc acccgggaat
     7261 ttccggcagc gcccgcggca gcagcgagcg ctgaatatcg ccgacgacct gcagttcctt
     7321 gtccagcgtc gccagcgctt tggccagatc gttccgcaag acgaggttcg tcgttccccg
     7381 gccgaatagt ccgctctgcc agtgcagcat cgggatgatc gagcgatcca attcatcgcc
     7441 gctcttgaat agcgaaatcg tgacgtttaa actttcgccg ccgtcgtact gtggcagcgc
     7501 gatcagcgac gcatatccat ccagataaaa cctcgcgggt tcgtcatccg ccagtcgcgc
     7561 tggcaaatca tcgatgaaga ctggcttgtt ggcgtagata atctcggcca acaatccgcc
     7621 ggaaaaccgc gggagcaacg cccgctgcgt ccaaggattg aaatgctccg tgaagcgcga
     7681 tcctcgcgtg atcaaatatt ccggcgaccg cagctcgcgc cgcgagagcg acagataatc
     7741 gcggatcggc agcagttcgc cgatgccttc ccagtaaata tgaaccagct cttccgggtc
     7801 ggtcacgtcc gagatcgcct tcatcgtgcg gtcgatgatg gccagctctt cctgccatgg
     7861 tcgtcgcttg ctcgacatgg tgggtatggt aacgacccgc ggcggcgaag gcgcgtgtgg
     7921 cgaacgaacg gagccccgga tgtacatccg gggcttcaac gccatgttgg cgacggtgct
     7981 gcttcgacgt agcgccggtt gtactcgggc acaacgccca gctccacgta tctccgctgc
     8041 acctccgccg gcaggtgttt gaaatcgcgg atgcgcctgc ggcaatttgg cgagccgcac
     8101 aggcagtcaa actcgaagtc gtcttcatcc atcgttgtcg agtaatcgaa cgtgacctgc
     8161 tcccccgcgg cgatgtcgcg caacgcgatc aactgcacgt cggtgccgtc gatgaccaag
     8221 cccgcgtcgg gatcgcacga gtgattgacg caatcgtcag cgccgccgga ttcgccgatg
     8281 tacaggtcga gaccgatctg cagcgccagc gtttgcggcg tcgtctgctc gtaacgcagc
     8341 agcggaccgg tgaatcgcag cagacgtgcc ccagctgaca aatgtgttga cgcaaacacg
     8401 ccgcggccgg ttgggctttc tcgaacgatc aggttcatgc ctcagctcct aaactgcgtt
     8461 cgctatctcg acggtggctg cgacgcctgc gtcgccgttt tcaaaccgca ccagagaggc
     8521 gacgcaggcg tcgcagctac aaagtcaaaa caacttcgtc attcgaaaat gcggccctgc
     8581 ggtagggatg tcgaacattt ccgatgcgat cgcccagccg agcgattgat aaaaccgcgc
     8641 cgccggcact cgggcgttcg cccacaggcg gtggatgtcc gaccgttcct gtcgcagcga
     8701 cgactcggcg aacgccagca gcgcccggcc gacgcctttg ctgcgaaatg cgtcatccgt
     8761 cgccatcccc cgcagttgcc aggcgggctc ggcctgccac ggattttcgt gaaaactcgc
     8821 gcagctgacg gcccggtccg tgtagaatgc ggcgaagtgc cgggtgcccg gcaggtcatc
     8881 tccggtgaag atcgccgact cacgcggcag gccgacgcgc agtacgcggt gccgcaggtc
     8941 cgcgatgtca gccagcgtgg cgcggcggat ctttacgacg ccgagatccg tctgacataa
     9001 tagatggtcc ggcggttcca cgcggttgaa ggacatacgc cacggcgggc agggtagcaa
     9061 gtcagacgga cgcttgttca acaagtcact tgagcgcgcc cgtcgagcgc gataggttta
     9121 tttcccaagt cctgtgggga ggccagatat gccaatcgtc gtagcatgca attgcggcaa
     9181 gcggttcaaa gcaggcgacc agttcgccgg caagcgcacg aagtgcccag gctgtgggca
     9241 agcgctgacc atccccggcg gcgcccccgc cgtcgcgacg gcggcgccac aaaagtccgc
     9301 agcgccaaaa gcggtcgctg ccgcagcgcc acccgctcgt cgcaccccag tcgcggcggc
     9361 gcgatcggcg ccggttcgcg ctgcgccccc accgccgctg ccggcgtttg aggatgatct
     9421 atcgacgctc ggcgagcccg aggaagtctt ccaacccgcc gcgcgaagcg aaaacgatgc
     9481 gccggtgaag acggtgaagc gcggcacatc cgcgctcgcc gccgccgccg cggttgcacc
     9541 acccggcaag cgcaaggccg gacccgcgtc cgccggcgcg ccgcgcttct ccatgtcgcc
     9601 cggcatgatc gtgctcgtcg tgctggcgat cctcatcccg tccaccatca cctgggtaaa
     9661 gctcggcccg atgaaggcgg ccgaagaatg gaacaagatc tcgtcgatcg ccgaagacaa
     9721 catctccggc cagatcgcgc gctcgctgct cagccagcgg gcaaagctcg gcttcgacgt
     9781 cagggacgtg aaatacatgc ccaagacgac caacttccag ttcgacgagt cgaaggcgtt
     9841 catgatgatc aagatgccgg agacggtacc gtttcagggt aagacgaccg atggcaactt
     9901 ctacggcacg ttccacccca agacgatgca gttcgaagcg gacgtcgaga tgatggacgg
     9961 caagcacaag ctgaccggaa gcgccagtga atcggataca tcacttgcga tcgacggcac
    10021 ggccgtgaag tagctgacac cctgccctga gcctgtcgaa gggtattccc gacgacccgg
    10081 atcggacgga gatgaaccca ctccgcgcgc accctccgcg ctccgcgtga ttgatcctcc
    10141 gtcttcgtta caatccgctt catgcccacc gcacaggaat cgatcgacgc agcgctcgtc
    10201 caccacgccg ccggacggtt ggtggaggcg gaagcgattt accgccaggt gctggcgcgc
    10261 gagccggaga accccgcagc cctgcacctg ctcggcgtcg cgtccagcca gatgggccgc
    10321 aaggacgt
//
LOCUS       DATIMH010000716         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3060890, whole genome shotgun sequence.
ACCESSION   DATIMH010000716 DATIMH010000000
VERSION     DATIMH010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060890"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1050
                     /locus_tag="VLI90_12610"
     CDS             133..1050
                     /locus_tag="VLI90_12610"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV15092.1"
                     /translation="MIRRRSHPVIPRRCAFTLVELLVVIGIIAMLIALLIPALNRARQ
                     QAQQVRCAAEIQQVVAVMTIHAISHHGYMPLAGLLEVPQANPAGLSDPTRIKYDYISF
                     PISGIDEPLIALPDALAKELGDPRIAGATSLNDLNAVHLDMTGFLKHFRCPSHAAMDP
                     TVLHTEMLYARDPFAPGASRSLTWRATQSYIFNEAAFGWDDNYARARGQLARIRRQSQ
                     TMAMADGVLGAPGRFPGAVGFATLYNKLALGPVTLADALAGNAKAGDPQNFDRQRHKG
                     TLNIGFFDGHVETRTIAVANLTDVYLLAP"
     gene            complement(1063..1332)
                     /locus_tag="VLI90_12615"
     CDS             complement(1063..1332)
                     /locus_tag="VLI90_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15093.1"
                     /translation="MLHRSLAARLAVVLLVLGVTFSAFAAAVAKKYQVTGKVLEVNDK
                     SITVEKGDEKWEIDRTADTKVDGDLKAGAKVTIHYHMVADSVDTK"
     gene            complement(1453..1656)
                     /locus_tag="VLI90_12620"
     CDS             complement(1453..1656)
                     /locus_tag="VLI90_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15094.1"
                     /translation="MSKLSPAGADLREQLDAALRELGEHARPPEHLEDAAAGAKSADG
                     MSPGTGSEEATSDPIVGASNFFG"
     gene            complement(1765..2700)
                     /locus_tag="VLI90_12625"
     CDS             complement(1765..2700)
                     /locus_tag="VLI90_12625"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HSV15095.1"
                     /translation="MDLIEQATTILRRGGLVAFPTETVYGLGADATNNDAVARIFKAK
                     GRPPTNPLIVHVATVAVARRYATTWPAIAEQLASRFWPGPLTLVLPKAKSIAPAATAG
                     RDSVGLRMPNHPLALAMLAGFDGPVAAPSANRSNHISPTQASHVRQELGDDVDLILDG
                     GPCDIGIESTVLDLTQTRPRILRPGGVSRAQIESIAGPVEMFEGTTNAAVAAASPGQH
                     ERHYSPRTPAYRLSAPGGIPASGATLVIGLRYYVQRPIRIEMPNDPIEYAKHLYTALR
                     EMDDWQVERIYVQMPPADPAWTAVRDRLMRATRAT"
BASE COUNT          502 a          937 c          940 g          550 t
ORIGIN      
        1 cgcgattgct tcgtcgacgg agtaccgccg acattcgtgg agtaccacgc accacgggcc
       61 agaggcgccg tgccacaatt ttgcttgtga atcacttccc ccagtcgtac gctactcggc
      121 agcggggacg agatgattcg ccgcagatca catcctgtaa tcccccgtcg ctgcgccttc
      181 acgctggtcg agctcctcgt cgtcatcggc atcatcgcaa tgctgattgc gctcctgatc
      241 cccgcgctca accgggcgcg gcaacaggcg cagcaggtgc gctgcgccgc cgagatccag
      301 caggtcgtgg cggtgatgac aattcacgcg atcagccatc atggctacat gccgctcgcg
      361 gggttgctcg aggtgccgca ggccaacccg gcggggctga gcgatccaac tcgcatcaag
      421 tacgactaca tcagcttccc catcagcggg atcgacgaac cgctgatcgc gctgccggat
      481 gcgttggcga aggagctggg ggatccgcgc atcgccggcg cgacgagctt gaacgacctg
      541 aacgccgtgc acctggacat gacgggtttc ctgaagcact tccgctgccc atcgcacgcc
      601 gcgatggatc cgaccgtgct gcacaccgag atgctctatg cgcgcgatcc attcgccccc
      661 ggcgccagcc gatcgctcac ctggcgggcg acgcagagtt acatcttcaa cgaggcggcc
      721 ttcggttggg acgacaatta cgcccgcgcc cgtgggcagc tggcccgtat ccgccggcag
      781 agccagacca tggcgatggc ggacggtgtg ctcggcgcgc ccggtcgatt tcccggcgcc
      841 gttggcttcg cgacgctcta caacaagctc gcgctcgggc cggtcacgct ggccgacgcc
      901 ctcgccggca acgccaaggc cggcgacccg cagaactttg accgccagcg ccacaagggc
      961 acgctcaaca tcggcttctt cgacggccac gtcgaaacgc gcacgatcgc cgtcgcgaat
     1021 ctcaccgacg tttatctgct ggccccgtga gcgctgcgcg aatcacttgg tatccacgct
     1081 gtcggcgacc atgtggtagt ggatcgtgac cttcgcgccc gccttcaaat cgccgtccac
     1141 cttggtgtcg gcggtgcgat cgatctccca cttctcgtcg cctttctcca cggtgatcga
     1201 tttgtcgttc acctcgagca ctttccccgt gacttgatat ttcttcgcca ctgccgcggc
     1261 gaacgcgctg aacgtcacgc ccaataccag caacaccacg gccaggcggg cagccaatga
     1321 tcggtggagc atcggatgtt cctcgaaaga gatggattta gtgcaacgct cccgcggaac
     1381 caggagcgcg cgagatcgtc gcacgccaga tgcagcaaac gggagcaaac ggcaacgcct
     1441 gaaccggcgt cttcagccga agaagttgga cgcgccgacg atcggatcgc tggtcgcctc
     1501 ttcgctgccg gttccgggtg acatgccatc cgccgactta gcgccggcgg cggcatcctc
     1561 caaatgttcg gggggcctgg catgttcgcc cagctcgcgc agcgcggcgt ccaactgctc
     1621 gcgaaggtcc gcgccggcgg gcgacagttt ggacataaag atcatcgttc acctcgctgt
     1681 cttacatata tgacatacgt tccgatgcgg gggaagataa aaaaagtgac cgcggcgcac
     1741 atttttgcag actgaaccgt tttctcacgt cgcccgcgtt gctcgcatca ggcgatcgcg
     1801 cacggccgtc cacgccggat cggccggcgg catctgcacg tagatccgct cgacctgcca
     1861 atcgtccatc tcgcgcaacg cggtgtacag gtgcttcgca tattcaatcg gatcattggg
     1921 catttcgatg cgtatcgggc gctgcacgta gtatcgcaat ccgatcacca atgttgcgcc
     1981 cgacgccgga atgccgccgg gggcgctgag gcgatacgcg ggcgttcgcg gcgaatagtg
     2041 ccgctcgtgt tgccccggac ttgcggccgc gacggcggcg ttcgttgtcc cttcaaacat
     2101 ctcgactgga ccggcgatcg attcgatctg cgcacggctc acaccgccgg gccgaaggat
     2161 gcgcgggcga gtttgcgtga ggtccagtac ggtggactcg atgccgatgt cgcacgggcc
     2221 gccgtcgagg atcaggtcca catcgtcgcc caactcctgg cgaacgtgtg acgcttgcgt
     2281 ggggctgatg tggttcgagc ggttggcgct gggggcggcg acggggccgt cgaagccggc
     2341 gagcatcgcg agcgcgagcg ggtgattggg catgcgcagg ccgacgctgt cgcgcccagc
     2401 ggtggcggca ggggcgattg atttggcctt gggaagcact aacgtgaggg gtccgggcca
     2461 gaagcgcgag gcgagttgct cggctattgc gggccatgtc gtggcgtatc gacgcgcgac
     2521 ggcgacggtg gcgacgtgga cgatcagcgg gttcgttggg ggacggcctt ttgctttgaa
     2581 gattcgcgca acggcgtcgt tgttggtcgc atcggcgcct aggccgtaga cggtttcggt
     2641 gggaaaggca accaagccgc cgcggcggag gatggtggtg gcttgctcga tcaggtccat
     2701 tgcttattca gcaaatacaa tacacgctgc gctcttgttt tgtcggcggc gtagcgccga
     2761 cgtgcgtgcg tacacgcacc acgggcagga tgcccgtgct actaaaggac cggcgggacc
     2821 cctttcagga atcccgccgg ccagtggttc gttgcggtgg accgtaagcc gaattctgtt
     2881 tcctcattgc tgaggagacg atcatttctc tgtgacgcgc gtcgccgcg
//
LOCUS       DATIMH010000717         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2480427, whole genome shotgun sequence.
ACCESSION   DATIMH010000717 DATIMH010000000
VERSION     DATIMH010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2480427"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VLI90_12630"
     CDS             <1..502
                     /locus_tag="VLI90_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15096.1"
                     /translation="HPGPLPDYRARGKEPHAIAQAPSPRNRFAIHHSRFSIYHALDMP
                     NPNHIPPPEMAKAFGVGKLTRHLFVCAGPDCVDPAAGEQTWNYIKRRMKDLNIAGPDG
                     PCYRTKCHCLRICTAGPIAVVYPEGAWYQNVTPANAERIIQDHLIGGRVVEDLCFATN
                     ALPARD"
     gene            complement(628..2067)
                     /locus_tag="VLI90_12635"
     CDS             complement(628..2067)
                     /locus_tag="VLI90_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15097.1"
                     /translation="MFPSRQSKIRRSGRITARAAAQAVHPEPLEPRWLFAGISGDVAA
                     GFTTFNAVGTANTTASIAAFQSAIGGVDNANVAAEQSNGSRRINWDGVKLDGTDFNGQ
                     TQTIVPNKVVGIPVNRFQTRGALFGEVYAVSGDGFASVNPGVAGQINPFSPKNTFGHF
                     NDIDIDTSFIKPSTATTSPVQQGTRGFGAIFLDVEQSNSSYIEYFHNNDSLGKFFVQP
                     GASGQPEFLGVLFNSPVVTKVEVHPGSSPLFFFHNGHVSAGQADISKGGTEDMAAVDD
                     FLYAEPASVANTVINHATEGKSFTGTVGKFTTSNSNPHASDFTATINWGDGTSTSAGT
                     VLTSGSGFAINGTHTYKEEGTFRVTVSIKDKSGHTGTARSISEVADAPLSSSGLSFNA
                     SNQAPFTGTVAKFSDADTSEIDATTYNALINWGDGTSTVGQIVSLGSGKWAVRGVHQY
                     ANAGTFNVKITIDDIGGSTTTASSTAHVI"
     gene            2334..3635
                     /locus_tag="VLI90_12640"
     CDS             2334..3635
                     /locus_tag="VLI90_12640"
                     /inference="COORDINATES: protein motif:HMM:NF025600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HSV15098.1"
                     /translation="MRQAKRRGAAVIVGLALVPFALPRSAIGCSLHWALGLPEPVGVW
                     PAELATSPTAPAQNSTVQTDTAAAASAAIAEINAADSVNDAAVTSWLQNPNNTKGFST
                     NSVVNSVVNKVNADVQTVAYTSTNVYVKATGIPSHTLGSYGSDPAIPTNVNRTVDIPR
                     NPVVNNGTKTNTGLGAIGVAVNGAVFYNAADAMSYNNANVWHQNANVFEASTFDGGPG
                     HPSPINGQTVTTSTGTAPAGNYHYHQAPTALINEIDPGNSGQHASPVIGFAFDGFPIF
                     GPYGYANADGTGGITLMSTSYQKRNITTRTTLPNGTTASSAGPDVSATYPLGSYLEDY
                     SYVAGSGTLDQYNGRFAVAPGYPSGTYGYYVTETAGGLPTYPYVVGPQYYGVVQSDDL
                     AGGTITVPANAVVYTPAVPEPSLLGLAACAIPTLLKRRSRG"
     gene            complement(3756..>4172)
                     /locus_tag="VLI90_12645"
     CDS             complement(3756..>4172)
                     /locus_tag="VLI90_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HSV15099.1"
                     /translation="AKLIKNAGAIVSHPYGSVMRSMLVAVYELKAAEVAVVGHYGCGM
                     TGLSCARLLEKAMARGVPPERLQELERDGIDLERWLVGFNSPEEGVRQSVALIRQHPL
                     LAPDVPVHGLMICPETGRLDVLIDGYASLPAPVTSH"
BASE COUNT          739 a         1404 c         1281 g          748 t
ORIGIN      
        1 tcaccccggc cctctcccgg attaccgggc gagggggaaa gagccacatg cgatcgccca
       61 ggcgcccagc ccacgcaatc ggttcgccat tcaccattcg cgcttttcca tttaccatgc
      121 gctcgacatg cccaatccca accacatccc cccgcccgag atggccaagg cattcggcgt
      181 tggcaagctg acccggcacc tgtttgtctg cgccggtcct gactgcgtcg atcccgccgc
      241 gggggagcag acgtggaact acatcaagcg gcggatgaag gacctgaaca tcgccggccc
      301 cgacgggccg tgctatcgca cgaagtgcca ctgcctgcgc atctgcaccg ccgggccgat
      361 cgcggtcgtg taccccgaag gcgcgtggta ccagaacgta acgcccgcca acgccgagcg
      421 gatcatccag gaccacctga tcggcgggcg agttgtcgaa gatctctgct tcgcgaccaa
      481 tgcactgccg gcacgagatt gacgatctca ttatcaaagc ccgcaaaaaa gcgcgcggct
      541 ccgccgcggg accctgcgcg aaaaatagag gacgtagctc ccgcgcccac cacacgagag
      601 ctacgtccgc ttagatcaac cgccggatca gatcacatgc gccgtgctcg acgcggtcgt
      661 ggtcgagccg ccgatgtcgt cgatcgtgat cttcacgttg aacgtgccgg cgttggcgta
      721 ctggtgcacg cctcggacgg cccacttgcc ggagcccagc gacacgattt gccccacggt
      781 gctcgtgccg tcgccccaat tgattagcgc gttgtaggtc gtcgcgtcga tctcgctggt
      841 gtcggcgtcg ctgaacttcg cgacggtgcc ggtgaacggc gcctgattcg acgcgttgaa
      901 gctcaacccg ctgctgctca gcggcgcatc ggccacttcg ctgatcgacc gggcggtgcc
      961 ggtgtgaccg gacttgtcct tgatcgaaac ggtgacgcgg aaggtgcctt cttccttata
     1021 ggtatgcgtg ccattgatcg cgaaaccgct gccgctggtg agcaccgtgc cggcgctggt
     1081 ggacgtgccg tcgccccagt tgatcgtcgc ggtgaaatcg ctcgcgtgcg ggttggagtt
     1141 gctcgtggtg aacttgccga ccgtgccggt gaaggacttg ccttcggtgg cgtggttgat
     1201 gaccgtgttg gcgacgctgg ccggctcggc gtagaggaag tcatcgacgg ccgccatgtc
     1261 ttcggtgccg cccttgctga tgtccgcctg cccggccgac acgtgaccgt tgtggaagaa
     1321 gaacaacggc gagctgcccg ggtgcacctc caccttcgtc accacgggtg aattgaacag
     1381 cacgcccagg aactcgggct gcccgctggc gccgggttgc acgaagaatt ttccaaggct
     1441 gtcgttgtta tggaagtatt cgatgtagct ggaattcgac tgctcgacat cgaggaagat
     1501 cgcgccgaac ccgcgcgtgc cctgctgaac cggcgatgtc gtcgcggtgc tgggtttgat
     1561 gaacgaggtg tcgatgtcaa tgtcgttgaa gtgaccgaag gtgttcttcg gcgagaacgg
     1621 gttgatctgc cccgccacgc ccgggttcac gctggcgaag ccgtcaccgc tgacggcgta
     1681 cacttcgccg aacagtgcgc cgcgggtctg gaagcgattg accggaatgc cgacgacctt
     1741 gttcggcacg atcgtctgcg tctgcccgtt gaagtccgtg ccgtcgagct tcaccccgtc
     1801 ccagttgatc cgccggctgc cgttggattg ctcggcggcg acgttggcgt tgtccacgcc
     1861 gccgatcgcc gattgaaacg ccgcgatgga cgcggtggtg ttggcggtgc cgacggcgtt
     1921 aaacgtggta aaccccgcgg cgacgtcgcc gctgatcccg gcgaacaacc agcgcggttc
     1981 cagcggttcc ggatgcaccg cctgcgcggc ggctcgagcg gtgatccgcc cgcttcgacg
     2041 aatcttcgac tgacgcgatg gaaacatgag aggctccttt caggatgcga tccccttcac
     2101 ccgctctccg ccggcaacca cgcggctgcc ccgcggacgg cgcacgctta tttgaaatgt
     2161 gcgtgtgagc gactgaattg gctgccacgt ctacgcaaag catctgacga tttgtgtgtc
     2221 gctgacaaga ttatttgtga cgtgaaaccg ccgttagctc ccccggcact aacggtgcac
     2281 aggcggcacg cgcggggctg cccaaacatc tgttcggatc aggaggcacg ggtatgcggc
     2341 aggcgaaacg tcgtggggcg gccgttatcg tgggtttggc gctcgtgccg ttcgctcttc
     2401 cccgcagcgc catcggctgc agcttgcact gggccttggg cttgcccgaa ccggtcggtg
     2461 tctggccggc cgagctggca accagcccca ccgcccccgc tcaaaattct accgtccaaa
     2521 ccgacaccgc ggccgccgcc tccgcggcga ttgccgaaat aaacgcggcc gactcggtta
     2581 acgacgccgc cgtcacctcc tggctgcaga atcccaataa caccaaaggc ttcagcacca
     2641 attccgtcgt caactccgtc gtcaataagg ttaacgccga cgtgcagacc gtcgcctaca
     2701 ccagcaccaa cgtctatgtg aaggcgaccg gtatcccgtc gcacacgctg ggttcctacg
     2761 gcagcgaccc cgccatcccc accaacgtca atcgcaccgt cgacatcccg cgcaatcccg
     2821 tcgtcaacaa cggcaccaag accaacacgg gcctgggcgc aatcggcgtg gccgtcaacg
     2881 gcgcggtctt ctacaacgcc gccgacgcga tgagctacaa caacgccaac gtctggcacc
     2941 agaacgcgaa tgtcttcgag gcttccacgt tcgacggcgg gccgggtcat ccttcgccga
     3001 ttaacgggca gaccgtcacg acctccaccg gcacagcccc ggcggggaac tatcactatc
     3061 accaagcccc aacggcgctg atcaacgaga tcgacccggg caactccggt cagcacgcgt
     3121 cgccggtcat cggcttcgcg ttcgatggct tccccatctt cggcccctac ggctacgcca
     3181 atgccgacgg caccggtggc atcacgttga tgtccacgag ttatcagaag cggaacatca
     3241 cgacgcgcac gacgctgccc aacggcacca ccgcgtcgtc ggccgggccg gatgtcagcg
     3301 cgacttatcc gctgggctcg tacctggagg actactcgta cgtcgccggg tccggcacgc
     3361 tcgaccaata caacggccgg ttcgccgtcg cccccggtta tcccagcggc acgtatggct
     3421 actacgtcac cgagaccgcc ggtgggctgc ccacgtatcc gtacgtcgtc ggcccgcaat
     3481 attacggcgt cgtgcaaagc gacgacctcg ccggcgggac gatcaccgtg ccggccaacg
     3541 cggtggtgta tacgcccgcg gtgccagagc cgtcgttgct cgggctggca gcatgcgcca
     3601 ttccgacgtt attgaagcgg cgctcgcgcg ggtgagagag ggatgattga acgcccccac
     3661 gccgggttcg gaggtactcc gacgacccgg attgtgcggc gcggcagggg aagaatccgg
     3721 gtcgtcgcga atacgctccg aacccggcgt gattgttagt gggaggtcac tggtgccggg
     3781 agcgacgcgt agccgtcgat cagcacatcc agccggccgg tttccgggca gatcatcaac
     3841 ccatgcaccg gcacgtcagg cgccagcaat ggatgctgcc ggatcagcgc gacgctttgg
     3901 cgaacgcctt cttcgggtga attgaagccg acgagccagc gctccaaatc gatgccgtcg
     3961 cgttccagtt cctgcagccg ttccggcggc acgccgcgcg ccatggcttt ttccagcagg
     4021 cgggcgcagc tcaggcccgt catcccgcag ccgtagtgac ccaccaccgc gacctcggcg
     4081 gccttcagtt catacaccgc gaccagcatg ctgcgcatca cgctgccata tgggtgcgag
     4141 acgatcgccc cggcattctt gatcagcttg gc
//
LOCUS       DATIMH010000718         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1330942, whole genome shotgun sequence.
ACCESSION   DATIMH010000718 DATIMH010000000
VERSION     DATIMH010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330942"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..576)
                     /locus_tag="VLI90_12650"
     CDS             complement(172..576)
                     /locus_tag="VLI90_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSV15100.1"
                     /translation="MTRTIKLTNWAAAGCLLTAAITGCHNATTKPTESASGVRSDVTD
                     IRPMESATSAYQPVAYQPTPAPAMDITPAPVAATPASATNGNTHVVKRGETLYSIAKS
                     SYGDGKQWQKIASANPGVSPASLKVGQTLMIP"
     gene            676..1674
                     /locus_tag="VLI90_12655"
     CDS             676..1674
                     /locus_tag="VLI90_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSV15101.1"
                     /translation="MAFISLALILLALGLVLVGLMMLLMARGILQPPRMTDWKAIDRL
                     HRLSPGDLGLTFENQNFTVRDARTHRPLKIAGWWIAHPAAAGRCAILIHGYADAKVGA
                     IAWAPLLHELGLNILAIDLRAHGDSGGFFSTGGFLERDDLNQVIDQLLAARADEARQL
                     ILFGISLGAAVAAATAARREDINAVILESPFADYRRIVTAHVSALGMPTGVVLRGALR
                     LAAVLSGADFDQVRPATTIRQTRAPLLIILGGDDELLEEQDVAALEQAVQNRASDAGP
                     AEIITADNATHLMAIHVDPAGYAARISSFIEHALADREPHLARREHTPGWVFGTSD"
     gene            complement(2049..>2517)
                     /locus_tag="VLI90_12660"
     CDS             complement(2049..>2517)
                     /locus_tag="VLI90_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HSV15102.1"
                     /translation="AQNSIWVPIMEKAFAFWRSGSNSYGSISGGSETEEFYALGGSSV
                     ASIWGNSAAATMNNINAALTGGKQVSVWTPNSDPTNGCPCVKNHVYTADHVNYQSVYV
                     PYIGVLTIPVSITLRNPWGTDGGGNNDGSNDGYVTVNANTFYGYFGGGDSAFV"
BASE COUNT          405 a          815 c          800 g          497 t
ORIGIN      
        1 tatcgtttag ccgccgcggc ctcgcggcgc ggacttcgag ttgcggcgct gttctaaaac
       61 cgcgccgcga ggccgcggcg gctaaacgcg ctgacgcgtt ctcaaaacga gaagccccgc
      121 atgtacatgc ggggcttcgt ctggtttccc aatgttcgtt cgcgccggcg atcacgggat
      181 catcagcgtc tggccgacct ttagcgacgc cgggctgacg cccgggttag ccgaggcgat
      241 cttctgccac tgcttaccgt cgccgtagct ggacttggcg atgctgtaga gcgtttcgcc
      301 gcgcttgacg acgtgcgtgt tgccgtttgt cgccgaagcg ggcgtcgcgg ccaccggcgc
      361 gggtgtgatg tccatcgccg gcgccggcgt tggttgatac gcgaccggtt gatacgccga
      421 tgtggccgat tccatcggcc tgatgtccgt cacgtcgctg cggacgccac tggccgactc
      481 cgtcggcttg gtggtcgcgt tgtggcaccc agtgatcgcc gcggtgagaa gacacccggc
      541 ggcggcccaa ttcgtcagtt tgatcgttcg cgtcatacgc cctgctcctg ttgaactgtg
      601 atgttcgaga ctatcggacg tttgggcatg cgacttgaat gattcctcgc tcacggactc
      661 tatactgcga tcgacatggc gttcatttcg ctggctttga tcctgctcgc cctgggcctc
      721 gtgctcgtcg gcctcatgat gctgctcatg gcccgcggca tcctgcagcc gccgcggatg
      781 acggattgga aggcgatcga tcgcctgcac cgcttgtcgc cgggcgacct ggggctgacg
      841 tttgagaacc agaacttcac cgtgcgcgat gcgcgaacac atcggccatt gaagattgcc
      901 gggtggtgga tcgcccaccc cgcggcggcg ggccggtgcg cgatcctgat ccacggctac
      961 gccgacgcga aggtgggcgc gatcgcctgg gccccgctgc tgcacgagct gggtttgaac
     1021 atcctcgcca tcgacctgcg cgcccacggc gacagcggcg ggttcttttc caccggcggg
     1081 tttttggaac gcgatgattt gaatcaggtg atcgaccaac tgctggcggc gcgagcggat
     1141 gaggcgcggc agttgatcct gttcggcatc agcctcggcg ccgccgtcgc cgccgccacg
     1201 gcggcgcggc gcgaggacat caacgccgtc attctggaat ctcccttcgc ggattatcgg
     1261 cggatcgtga ccgcccacgt cagcgcgctc ggcatgccga ccggggttgt ccttcgcggt
     1321 gcattgcgcc tggctgcggt gttatccggc gcagatttcg atcaggtgcg gcccgccacg
     1381 acgatccggc aaacgcgcgc gccgctcctg atcatcctcg gcggcgacga tgaattgctc
     1441 gaggagcagg acgtcgccgc gctggaacaa gcggtgcaga atcgcgcgag cgacgccggt
     1501 ccggcggaga tcatcacggc cgacaacgca acacacctga tggcgatcca cgtcgatccc
     1561 gccggctacg ccgcgcggat ctcgtcgttc atcgagcacg ccctcgctga tcgagaaccc
     1621 catttagccc ggcgtgaaca cacgccgggt tgggtgttcg ggacctctga ttgagaattc
     1681 gaccggcctc gacttcttaa cgtaactcgg cgtgtgttca cgccgggcta ctgaccgccg
     1741 gctagataag gcgcgcggct gctgacgcac ggtcttgtcc cctctcccac gtactcacgg
     1801 gagagggccg gggtgagggt gatttcgaac gtcgagcggc tttcgaagtt ccaaatcacc
     1861 ctcaccccgg ccctctccca cgagtacgtg ggagaggagg gaaaggccaa gacgcgcttt
     1921 aacaaccgcg cgctcagtaa atgggggcac cttcgtttcc gggctcgcct aacaaagcaa
     1981 gaggcctccc ttgttcaggg aggcctctgc atacaaccca cgcctattta tccgacgact
     2041 cagcgcgatt acacgaacgc cgaatcgccg ccaccgaagt agccgtagaa cgtgttggcg
     2101 ttcacggtga cgtagccgtc gttgctgccg tcattgttcc cgccgccgtc ggtgccccac
     2161 gggttgcgga gcgtgatcga caccgggatg gtcaacacac cgatgtacgg cacgtacacc
     2221 gactgatagt tcacgtggtc ggcggtgtac acgtgattct tcacgcacgg gcagccgttg
     2281 gtcgggtcgc tgttgggcgt ccagacgctg acctgcttgc caccggtcaa cgcagcgttg
     2341 atgttgttca tcgtcgccgc cgcgctgttg ccccagatcg aggcgacgct gctgccgccc
     2401 agggcataga actcctcggt ctcgctgcca ccgctgatcg acccgtacga gttactgccg
     2461 ctgcgccaga aggcaaacgc cttctccatg atcgggaccc agatcgagtt ctgggca
//
LOCUS       DATIMH010000719         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5084728, whole genome shotgun sequence.
ACCESSION   DATIMH010000719 DATIMH010000000
VERSION     DATIMH010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5084728"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2586)
                     /locus_tag="VLI90_12665"
     CDS             complement(<1..2586)
                     /locus_tag="VLI90_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15103.1"
                     /translation="MSNRTPAAALAVAAAVGLLGLFPRPACAIIVEQIEAYSDAVAPY
                     SHYTPIDFAGQSTVTVNDGGMFNGIAFHALRATTNTFSNHANLVGSLVYGSSSVANPY
                     VTDVYVGESTSFIWYTAGASNVQSTSGSLPNHFVSGAQVINNSYIASLSNTLDCQRRI
                     DFMINRDDVTFLAGAANPPPSVSTFVTWSSYNALAVRGLQSTFDPTNSPGKTHADLCL
                     DGRASFTAAIVSGYAAGLYGQAQSAGQTDAAHGVVMRSLLMAGADKTLYARQTANNLD
                     VENGAGQPNYYTSLSMLQNGETPFDNVAADGTISGGFDPSLRGWSYGTIPGGSANQSA
                     VLIHVPAGGARGVTASLNWNVTQNQPTAGTIDTTDAGVVFPKLSLELRPVNTISKNGV
                     SSLALGPSLNDPTLSSAAANDNVQYLYSTNTLPAGDYAFVITGDASAARSAAVGFSYS
                     VLAPVRSQWSAVAGGSWSSASNWSGGVPTYGADRATFSSASGFTGTIAVTLDGNRSIG
                     QLTFGDAHGFSIGSGAGGGTLTIDDTGDAPGVTPLITVAAGNQRIGAPLALAIGATAN
                     IAVGASLNLAGPVIGSGNLTKTGGGSLTLSGAGSSFGDTLVTAGSFTLSSGASIARGN
                     LSVSPGATVAINGSLPASATVTAGGATVFAGNNAQQPRIVTLASLQIGSNTIVTVAPS
                     ASAMSPTVLAPAVVGFTDAGSILNLSNNELITTEPFATARQWIISGQIIGTSTSTERL
                     GSMDLDNGQSEIRCTLFGDANLDGQVGVQDLGAFSTNYGRTSGARWQDGDFSYDGAVN
                     VSDLGYIATFFGSTLSSMGVSSPAGPTAIAATVPEPVCVIPLVLLMTTLRRSRGALAR
                     EPSRVR"
     gene            complement(2774..>3140)
                     /locus_tag="VLI90_12670"
     CDS             complement(2774..>3140)
                     /locus_tag="VLI90_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15104.1"
                     /translation="SLAGTVKNVTAGSGDNVTFQSTSNPSGTSTPVQSAVLPAAGTSP
                     VPFTASPATTTATFVRGAAFDLSNTTTITLSSGNESTNLSGTTTAVSQGPAIPEPTTL
                     TLLGLAALPMLHRRPRRRA"
BASE COUNT          520 a          938 c         1131 g          551 t
ORIGIN      
        1 tcgaacgcgc gacggttcgc gggccaaagc cccgcggctg cgacgtaatg ttgtcatgag
       61 gaggacgagg gggatcacgc aaaccggctc gggaaccgtc gcggcgatcg cggtgggacc
      121 cgcggggctc gacacgccca tgctgctgag cgtcgagccg aaaaacgtgg cgatgtagcc
      181 caggtccgaa acattcaccg cgccgtcgta gctgaaatcg ccgtcctgcc agcgggcgcc
      241 gctggtccgc ccgtaattcg tcgagaacgc gcccaggtcc tgcacgccga cttggccatc
      301 gaggttcgcg tcgccaaaca gcgtgcagcg gatttcggat tggccgttgt cgagatccat
      361 cgagccaagc cgctcggtgc tcgtgctggt gccgatgatc tggccggaga tgatccactg
      421 acgcgcggtg gcgaacggct cggtcgtgat caactcattg ttcgacaggt tgagaatgga
      481 tccggcatcg gtgaaaccca ccacggccgg cgcgagcacc gtcggcgaca tcgccgacgc
      541 cgacggcgcg acggtcacga ttgtgttgct gccgatctgc agcgacgcaa gcgtaacgat
      601 gcgcggctgc tgggcgttgt tgcccgcgaa gacggtcgcg ccaccggcgg tgacggtggc
      661 gctggcgggc aacgagccat tgatcgccac cgtcgcgccg ggggagacgc tcaggttccc
      721 gcgggcgatc gaggcgccgc tgctcaacgt gaagctgccg gcggtgacca gcgtatcgcc
      781 aaagctgctg ccggcgccgc tgagcgtgag cgaccccccg ccagtcttgg tcaggttacc
      841 gctgccgatg accggaccgg ccaggttcag cgacgcgccc acggcgatgt tggcggtggc
      901 gccgatggcg agggccagcg gcgcgccgat gcgctgattg cccgcggcga cggtgatcag
      961 cggcgtgacc cccggggcgt cgccggtgtc gtcgatcgtt agggtgcccc cgccggcgcc
     1021 gctgccgatg ctgaagccgt gagcatcgcc gaaggtgagc tggccgatcg agcgattgcc
     1081 gtcgagtgtg acggcgatgg ttccggtgaa accgctggcg gagctgaagg tcgcgcgatc
     1141 cgcgccgtac gtcggcacac cgccgctcca gttcgatgcg ctcgaccacg accccccggc
     1201 aacagcggac cactgcgacc tcacgggagc gagcacactg tacgaaaatc caaccgcggc
     1261 cgatcgggcg gcgctggcgt cgccggtgat gacgaatgcg taatcgccgg ccggcagcgt
     1321 gttggtggaa tagaggtatt gaacgttgtc gttggcggcc gcgctggaga gtgtcggatc
     1381 gttcaagctc gggccgagcg ccaggctgct gacgccgttc ttcgagatcg tgttcaccgg
     1441 gcgcagttcc agcgacagct tggggaagac gacgccggca tcggtggtgt cgatcgtgcc
     1501 ggcggtgggc tggttctgcg tgacgttcca gttgagtgat gcggtgacgc cgcgcgcgcc
     1561 accggcgggg acgtggatca gcaccgccga ttggttcgcg ctgccgccgg ggatggtgcc
     1621 gtaggaccaa ccgcgcaagc tggggtcgaa cccaccgctg atcgtgccgt cagcagcaac
     1681 gttatcgaac ggcgtttcgc cgttctgcag catcgacagg ctcgtgtagt agttgggctg
     1741 gcccgcgccg ttttcgacgt ccagattgtt ggcggtctgc cgggcgtaaa gcgtcttgtc
     1801 cgcgccggcc atcagcaggc tgcgcatcac caccccatgc gcggcatcgg tctgcccggc
     1861 cgactgtgcc tggccataca acccggcggc gtaaccagag acgatggcgg cggtgaagct
     1921 ggcccggcca tccaggcaca agtcggcatg cgtcttgccg ggcgaattgg tcggatcgaa
     1981 ggtgctctga agcccgcgca ccgccaaggc gttgtaggaa ctccaggtga caaacgtgct
     2041 gaccgaaggc ggtgggttgg cggcgccggc caggaacgtg acgtcgtccc ggttgatcat
     2101 gaaatcgatc cgccgctggc aatcgagcgt gttgctcagg ctcgcgatgt agctgttgtt
     2161 gatgacctgc gcgccgctga cgaaatggtt cggcagcgac ccgctggtcg attgcacgtt
     2221 cgacgcgccg gcggtgtacc agatgaagct ggtgctctcg ccgacataga cgtcggtcac
     2281 ataagggttg gcgacggagc ttgagccgta aaccagcgaa cccaccaggt tggcgtggtt
     2341 ggagaaggta ttggtggtgg cccgcagggc gtggaaggcg atgccgttga acatcccgcc
     2401 gtcgttgacg gtgacggtgc tttgaccggc gaaatcgatg ggggtgtagt ggctatacgg
     2461 cgcgacggcg tccgagtaag cctcgatctg ctcgacaatt atagcacaag ccgggcgtgg
     2521 gaagaggccg agcagcccca cggccgctgc caccgcgagt gcggcggcgg gggttcggtt
     2581 ggacattctc caagctccgc gtcccccgcg ggtttcctcg tccgtcagta gaattgctta
     2641 gtgtacgatt tgaacgtcca cgtcgttcga aagcaaggtg gatcgttgaa gatgtttaaa
     2701 aaatgttgcg cctcggaaaa agaactagcc gctgaagcgg ctagtccggt cgtttatgtt
     2761 gattctttca ggctcaggct ctgcgacggg ggcggcgatg gagcatcggg agcgccgcca
     2821 atccgagtag cgtcagcgtc gtcggttcgg ggatggcggg gccttgggag actgcggtgg
     2881 tcgtgcccga caggttggtg gattcgttgc cgctggagag cgtgatcgtc gtggtgttcg
     2941 agaggtcgaa cgccgcgccg cggacgaagg tcgccgtggt ggtcgcgggc gaagccgtga
     3001 agggcaccgg agaagtcccc gcggccggca gcaccgcgct ctgaacgggc gtgctggtgc
     3061 cgctgggatt ggacgtgctc tggaaagtca cgttatcgcc ggacccggcc gtcacgttct
     3121 tcacggtgcc ggcgaggctg
//
LOCUS       DATIMH010000720         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2481735, whole genome shotgun sequence.
ACCESSION   DATIMH010000720 DATIMH010000000
VERSION     DATIMH010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481735"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="VLI90_12675"
     CDS             complement(<1..2226)
                     /locus_tag="VLI90_12675"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HSV15105.1"
                     /translation="MDNAVHISTDADRVMTVTMDLPGKPVNTCSSQMLDELSQAIDKI
                     ERERPAGVIFASAKARSFNAGADLFDIRKMSRDQTAEYLKRGQSLFARIASLPVPTIA
                     AINGDTLGGGCELALACTYRVAVDDTSISIGLPEVKLGLIPAWGGTTRLPRLIGLRNA
                     LPILLAGKTMPPRKAQRAGLVDEVVRREALLAAAKRIVLSKHAPHRPARLDRAADKLA
                     VARNRMIDTGRRKTMAMTYGNYPAPLSLLDVLRAGYERGLDAGLEAERQAIIDLTATD
                     TGRNLLRVFFLRQGAKKAATGCLTGGVAATPASPVAAAGTQASQLQEQQSTGPEVRYA
                     AVIGGGTMGAGIVHALIRAGIPVRLVEVNPQAVSAALGRIKKMLDDDVAMGKLDKLAA
                     RHTFNRVSPTTDWTGLELADFVVEAVLETIEAKREVFSKLDRLCRPTAVLATNTSSLR
                     LADIEQATLHPERIVGLHFFNPVPKMPLVEVVRGPHSDDASLATAIALAGRMGKTPVL
                     VNDAPGFLVNRILVPYLAEALLMASEGRSILSIDDAMKRWGMPMGPFELLDEIGLDIA
                     AHVLKSLGGVSPPPAKVVAAIGQAQQRKWLGKKTGRGFYVHRDKRGAKPVLNEELGAM
                     LAPTAAGPLSPDESLRESIQWRLVLPMVNEAARAIEEGVTDSTDTIDLAMVMGTGFAP
                     FRGGLMQFANTTGAEEIVRRMDELAGKRGARFEPAKLLREAARERRTIGASPPTAIET
                     TV"
     gene            complement(2419..3741)
                     /locus_tag="VLI90_12680"
     CDS             complement(2419..3741)
                     /locus_tag="VLI90_12680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSV15106.1"
                     /translation="MVAWNTTTSSSNGGARVAIVAGARTPFVKMGGPLRDMHAADLAK
                     TTFQETLYRAHWPMDRLDEVLLGNVVMPVDAANLARVSALWAGIPAKVPAMTVQRNCA
                     SGMESIADAAARIRSGQERVVLAGGAESMSTIPLLFPPETMEPMARMARSKSMWQRAA
                     AVATLRPRHFRPVAALEKGLSDATVGMNMGKTAEVLAQEFGISRREQDEFALRSHQRA
                     VAAAAAGKFKDEIVPVYAGKRFEPITADIGPRTGQTLEALAKLKPIFDRRDGSVTVGN
                     SCQVTDGAATVLAMDAELAKTEGLEPLGYVRAYAYAALDPAHMGLGPVFAIDQLLRST
                     GLTLNDIPLIEINEAFAAQVLACLKAMASRRFAAQHLNHSAPVGEVDPDRLNVNGGAI
                     ALGHPVGATGARLILTLLHEMRRRDVDLGIASLCVGGGQGAAILLERR"
     gene            complement(3741..>4860)
                     /locus_tag="VLI90_12685"
     CDS             complement(3741..>4860)
                     /locus_tag="VLI90_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSV15107.1"
                     /translation="QPTSGTTGALKLVELSHRNLLSNATQIAVWMAARDGQERVLSVL
                     PMFHVYGLMTGLLNPIFIGATLIIMTRFECEEALDILLREHPTVFPLVPAICTSLSDE
                     IEQRHPRPALQGLRVCISGAAPLPRDVAERFERLTGAHVIEGYGLSETSPVTHANVLG
                     RPRYGCIGLPLPDTRCRVVDLDDETKDAAIGQPGELLISGPQVMSGYYGNPDASAKAL
                     VKDDAGHVWLRTGDIARVDEDGFFQILERRKDMIIHSGLKVYPAKVERVLANHPQVAD
                     VSVIGAPDAVHTEEVTAFIVQIDPTVHDRAKLTLELRAHCREHLAPYEVPSRFEFIEQ
                     IPRNILGKALKKVLREQLNKPADPPPQPEPNRHQKEAA"
BASE COUNT          771 a         1676 c         1543 g          870 t
ORIGIN      
        1 aacagttgtt tcgatggcag tcggtggcga tgcaccgatc gtccggcgct cgcgcgccgc
       61 ttcgcgcagc agcttcgcgg gttcgaagcg cgccccacgc ttccctgcca gctcgtccat
      121 ccgccgcacg atttcttccg cgccggtggt gttcgcgaac tgcatcagcc cgccgcggaa
      181 gggggcgaag ccggtgccca tgaccattgc cagatcgatc gtgtcggtag aatcggtcac
      241 gccttcctcg atcgcgcggg cggcttcgtt caccatcggc agcaccagcc gccactggat
      301 tgattcacgc agcgattcgt ccggcgacaa cggccccgcc gccgtcggcg ccagcatcgc
      361 ccccagctct tcgttcagca cgggcttggc cccgcgtttg tcccgatgca cataaaaccc
      421 gcggccggtt ttcttgccga gccacttgcg ctgctgtgcc tgaccgatgg ccgcgacaac
      481 cttcgccggc ggcggcgaga caccgcccag gctcttcagc acatgcgcgg cgatgtccag
      541 cccgatttca tcgagcagct cgaacggccc catcggcatg ccccaacgct tcatcgcgtc
      601 gtcgatcgac aagatcgacc tgccttcgct ggccatcagc agcgcctcgg ccaggtacgg
      661 caccagaatg cggttgacca ggaatcccgg cgcatcgttc accagcaccg gcgtcttgcc
      721 catccgcccg gcgagcgcga tggcggtcgc cagcgacgca tcatcactgt gtggcccgcg
      781 cacgacttcg accagcggca tcttcggcac cgggttgaag aagtgcagac ccacgatccg
      841 ctcggggtgc agcgtcgcct gctcgatgtc cgccagccgc agcgaactcg tattcgtcgc
      901 cagcaccgcg gtggggcgac agaggcggtc gagcttcgag aaaacttcgc gcttggcttc
      961 gatcgtctcc agcaccgctt cgacgacgaa atccgccaac tccaggcccg tccaatcggt
     1021 cgtcggcgag acgcggttga acgtgtgccg ggcggccagc ttgtcgagct tgcccatggc
     1081 gacatcgtca tcgagcattt tcttgatccg ccccagtgcc gctgaaacgg cctgtgggtt
     1141 cacttcaaca agccgcaccg gaatgccagc gcggatgaga gcgtgcacga tgccggcacc
     1201 catggtcccg ccgccgatga cggcggcgta gcgaacttct ggtcctgtcg actgctgctc
     1261 ctgtagctgc gacgcctgcg tccccgctgc cgcaacaggc gacgcaggcg tcgcggctac
     1321 acccccggtg aggcagcccg tcgccgcctt cttcgcccct tggcgaagga agaacacgcg
     1381 cagcagattc cgcccggtgt cggtggcggt gagatcgatg atcgcctgcc gctcggcttc
     1441 gaggcccgcg tcgaggccgc gctcgtagcc ggcgcggagg acgtccagca gcgacagcgg
     1501 cgcggggtaa ttgccgtacg tcatcgccat tgtcttgcga cgcccggtgt cgatcatgcg
     1561 attgcgcgcg accgcgagct tgtcagccgc gcgatccagc cgcgcgggcc gatggggtgc
     1621 atgcttggaa agcacgattc gcttcgccgc ggcgagcagc gcttctcgcc gcacgacttc
     1681 atcgaccagt cccgcgcgct gggccttccg cggcggcatc gttttgccag ccagcaggat
     1741 cggcagcgca ttgcgcaggc cgatcaatcg cggcagccgg gtggtgccgc cccaggcggg
     1801 gatcagcccg agcttcactt ccggcaggcc gatcgagatc gacgtgtcat cgacggcgac
     1861 gcgatacgtg cacgccagcg ccagctcaca cccgccaccc agcgtgtcgc cgttgatggc
     1921 cgcgatcgtc ggcaccggca gcgacgcgat gcgcgcaaac agcgattgcc cgcgcttcag
     1981 atattctgcc gtctggtcgc ggctcatctt gcggatgtcg aacaaatccg ctccggcgtt
     2041 gaacgaacgc gctttggcgg acgcgaagat gacgcccgcc ggccgctcgc gctcgatttt
     2101 gtcgatcgcc tgcgacagct catcgagcat ctgcgatgag caggtgttga ccggcttgcc
     2161 cggcaggtcc atcgtgaccg tcatcacgcg atcggcgtcg gttgaaatat gaaccgcatt
     2221 gtccatgttt gctccaatgt ggcatgggca tcttgcccat ggtgcgtgta cgcacgcacg
     2281 gtcgatgtac tcatcgactc agccagagcg cagcgtccaa acgaacaatc gacgctgcgc
     2341 tcttgcttcg tcggcggcgt agcgccgacc tgcgtggcgt accacgcatc atgggcaaga
     2401 tgcccatgcc acggtcgttc atctacgttc cagcagaatc gccgcgcctt ggccgccgcc
     2461 gacgcatagg ctggcgattc ccaggtccac atcgcgtcgg cgcatttcgt gcagcagcgt
     2521 caggatcaat cgagcgcccg tcgcgccgac gggatgaccc agcgcgatcg cgccgccgtt
     2581 gacgttgagc cgatcgggat cgacttcgcc gacgggcgcg ctgtgattca ggtgctgggc
     2641 cgcgaagcgt cgcgaagcca tcgccttcag gcacgccagc acctgggcgg cgaaggcttc
     2701 gttgatttcg atcagcggga tgtcgttcaa agtcaggccg gtcgatcgca gaagctgatc
     2761 gatggcgaag accggaccaa ggcccatgtg cgccggatcg agggcggcgt atgcgtacgc
     2821 ccgcacgtaa ccgagcggct ccaatccttc ggtcttggcc aactccgcgt ccatcgccag
     2881 cacagtcgcg gcgccatcgg tgacctggca tgaattgccc accgtcacgc tgccgtcgcg
     2941 gcggtcgaag atcggcttca acttcgccag cgcttccagt gtctgcccag tgcgcgggcc
     3001 gatgtcggcg gtaattggct cgaatcgttt ccccgcgtac accggcacga tctcatcttt
     3061 gaacttccca gccgcggcgg cggcgacggc tcgctggtgt gaccgcagcg cgaactcatc
     3121 ctgctctcgc cgcgagatgc cgaactcctg cgccagcacc tcggcggtct tgcccatgtt
     3181 catgccaacc gtcgcgtcgc tcagtccttt ctccagcgcg gcgaccggcc ggaagtgccg
     3241 cgggcgaagc gtggcgaccg cggcggccct ttgccacatg ctcttggacc gcgccatacg
     3301 cgccatcggt tccatcgtct ccggcgggaa cagcagcggg atggtgctca tcgactcggc
     3361 accgccggcc aacaccaccc gttcctgccc cgagcgaatt cgagccgcgg catcggcaat
     3421 ggattccatg ccggatgcgc agttgcgctg caccgtcatc gccggcacct tcgccggaat
     3481 gcccgcccac agggccgaca cgcgcgccag gttggccgcg tccaccggca tcaccacatt
     3541 gccgagcagc acttcgtcca accggtccat cggccagtgc gcgcgataga gggtttcctg
     3601 aaatgtcgtc ttcgccaggt cggccgcgtg catgtcgcgc agcggcccgc ccatcttgac
     3661 gaacggcgtg cgcgccccgg cgacgatggc gacgcgcgca ccgccattgg acgacgaagt
     3721 tgtcgtgttc cacgcgacca ttacgcggcc tccttctgat gtcggttggg ttccggttgc
     3781 ggcggcgggt cggctggttt gttcagctgt tctcgcagca ccttcttcag cgccttgccc
     3841 aggatgttcc gcgggatctg ctcgatgaat tcgaaccgcg acggtacctc gtaaggcgcc
     3901 aggtgctcgc gacagtgcgc gcgaagttcc agcgtcagct tcgcgcgatc gtgcactgtc
     3961 ggatcgatct gcacgatgaa cgcagtcact tcctcggtgt gcacggcatc gggcgcgccg
     4021 atgacggaca cgtccgccac ctgcgggtgg ttcgccagca cgcgctcgac cttggccgga
     4081 tagaccttca ggccggagtg gatgatcatg tccttgcggc gttcgaggat ctggaagaac
     4141 ccgtcctcat cgacccgggc gatgtcgccg gtgcgcagcc agacgtggcc ggcatcgtcc
     4201 ttcaccagcg ccttggccga agcgtcaggg ttgccgtagt aaccgctcat cacctgtgga
     4261 ccgctgataa gcaattcgcc tggctggccg atggcagcgt ccttggtctc gtcgtccaga
     4321 tcgaccacgc ggcagcgcgt gtccggcagc ggcaggccga tgcagccata gcgcgggcga
     4381 ccgagcacgt tggcgtgcgt gaccggcgat gtttcgctca ggccgtagcc ttcgatgacg
     4441 tgcgcgccgg tcaatcgctc gaagcgctcg gccacatcgc gcggcagcgg ggcggcgccg
     4501 ctgatgcaga cgcgcagccc ttgcagcgcc ggccgcgggt ggcgctgctc gatctcatcg
     4561 ctgagcgacg tgcagatggc cggcaccagc gggaatacgg tggggtgttc gcgcagcagg
     4621 atgtcgagcg cctcttcgca ttcgaagcgg gtcatgatga tcagcgtcgc gccgatgaag
     4681 atcggattca gcaggccggt catcagccca tatacgtgga acatcggcag caccgacagc
     4741 acgcgttcct gtccgtcacg cgcggccatc cacacggcga tctgcgtcgc gttgctcagc
     4801 aggttgcggt gcgacagttc caccagcttc agcgcgccgg tggtgccgct ggtcggttgc
//
LOCUS       DATIMH010000721         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1041119, whole genome shotgun sequence.
ACCESSION   DATIMH010000721 DATIMH010000000
VERSION     DATIMH010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041119"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VLI90_12690"
     CDS             complement(<1..669)
                     /locus_tag="VLI90_12690"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HSV15108.1"
                     /translation="MVKALRPVKISGLATYVPPKVLSNADLEKLVDTSNEWILQRTGI
                     RERHIVDPGVATSDLAKEAAIGAMHQAGVSPDQIGFIVVGTTTPDTIFPSTACMLQHK
                     IGARHAWGFDLGAACSGFTYALTTGMQMIAAGAHDHVLVVGADVMSSIIDYTDRTTCV
                     LFGDGAGAVVLSAAADGEPHILDFAHEIDGSGGPALCMPAGGSRLPASHDTIDQRLHY
                     VKQDG"
     gene            complement(663..>1649)
                     /gene="ruvB"
                     /locus_tag="VLI90_12695"
     CDS             complement(663..>1649)
                     /gene="ruvB"
                     /locus_tag="VLI90_12695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HSV15109.1"
                     /translation="RGEDEDQYEAGLRPRTLDDYIGQERVRENLHVSITAATQRGEAL
                     DHVLLWGPPGLGKTTLATVIANELGVPIRATAGPVIEKAGDLAAMLTNLQARDVLFID
                     EIHRMAPAIEEILYPAMEDYELDIIIGQGPSARSVKLPLQRFTLIGATTRTGLLTGPL
                     RARFGIVHRLDFYKDDEIREIVERSARILGVPIDRDAAIEIAHRSRGTPRVANRLLRR
                     VRDYAQVRASGHITLDVARRALSLLDVDERGFDDLDRRLLRTIIEKFNGGPVGVASLA
                     AALSEERDAIEDIYEPFLIQIGFLDRTPRGRVATASAYAYFGLDAPGKDRLW"
BASE COUNT          280 a          557 c          516 g          296 t
ORIGIN      
        1 accgtcctgc ttcacataat gcagacgctg atcgatggtg tcgtgcgagg ccggcagccg
       61 gctgcctccg gccggcatgc agagcgcggg cccgccgctg ccgtcaatct cgtgggcgaa
      121 atcgaggatg tgcggctcgc cgtcagccgc ggccgagagc acgaccgccc cggcaccgtc
      181 gccgaagagc acgcacgtcg tgcggtcggt gtaatcgatg atgctcgaca tcacatccgc
      241 gccgacgacc agcacatgat cgtgcgcccc ggcagcaatc atctgcatgc cggtggtcag
      301 cgcataggta aacccggaac aggccgctcc caggtcgaaa ccccacgcgt gacgcgcgcc
      361 gatcttgtgc tgcagcatgc acgcggtact tggaaagatc gtgtcagggg tggtggtgcc
      421 aacgacaatg aagccaattt ggtcgggcga caccccagcc tggtgcatcg cgccaatcgc
      481 tgcctctttc gccagatccg aggtggcgac gcctggatcc acgatgtggc gctcgcggat
      541 gccagtccgt tgaagaatcc actcattgct ggtgtcgacc agtttctcca ggtccgcgtt
      601 cgacaacacc ttcggcggaa catacgtggc cagtcctgag atcttgaccg gcctgagcgc
      661 cttcaccaca aacggtcctt tcccggggca tccaacccga aatacgcata cgcgctcgcc
      721 gtcgccaccc gccctcgcgg cgtccggtcg aggaatccga tctgaatcag gaatggctcg
      781 tagatgtcct cgatcgcgtc gcgctcttcg ctgagtgcgg cggccaggct ggcgacgccc
      841 acgggccccc cgttgaactt ctcgatgatc gtgcggagca gccggcgatc gagatcgtcg
      901 aacccgcgtt cgtcgacatc gagcagcgac agcgcgcgtc gtgccacatc cagcgtgatg
      961 tgcccgctcg cgcgcacctg cgcgtaatca cgaacccgcc gcagcagccg gttcgccacc
     1021 cggggcgtcc cccgcgagcg gtgcgcaatt tcgatcgccg catcgcgatc gatgggcacg
     1081 ccaagaatcc gtgccgagcg ttcgacaatc tcgcggatct cgtcgtcttt gtagaagtcg
     1141 agccggtgga cgatcccgaa ccgcgcgcgc aacggcccgg tcaacagtcc ggtgcgcgtg
     1201 gttgcgccga tcagggtaaa tcgctggagc ggcagcttga ccgagcgcgc gctgggcccc
     1261 tgaccgatga tgatgtcgag ctcgtaatcc tccattgcgg gatagaggat ttcttcgatt
     1321 gccggcgcca tgcgatggat ttcgtcgatg aagagcacgt cgcgcgcctg caggttcgtc
     1381 agcatcgccg cgaggtcgcc cgctttttca atcaccgggc cggcggtcgc acgaatcggg
     1441 acgcccagtt cgttggcaat cacggtggcc agtgtggtct tcccgagtcc cggcggcccc
     1501 cacagcagca cgtgatcgag cgcctcacct cgctgcgtcg ctgccgtgat cgagacgtgg
     1561 agattctcgc gtacgcgctc ctggccgatg tagtcgtcga gggtccgcgg ccgaagcccc
     1621 gcttcgtact gatcctcgtc ttcgccgcg
//
LOCUS       DATIMH010000722         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1910423, whole genome shotgun sequence.
ACCESSION   DATIMH010000722 DATIMH010000000
VERSION     DATIMH010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910423"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            593..1117
                     /locus_tag="VLI90_12700"
     CDS             593..1117
                     /locus_tag="VLI90_12700"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEPxxWA-CTERM sorting domain-containing protein"
                     /protein_id="HSV15110.1"
                     /translation="MAAAAALTCASASNAAITVEGHSSDVSVGTPNNSTIPNKVNFDS
                     VTNTAATINPWFDFKNDQDGFYVFDLTSSTLYSSITLEKVLSDGGTTLVNQVIGSGRF
                     LELITPTLDAGTVYRFTYTYNTPDGGGTVSGNASFYPAVPEPATWAMMLIGFGGIAMA
                     MRRRGQGMALAQVA"
     gene            1399..1953
                     /locus_tag="VLI90_12705"
     CDS             1399..1953
                     /locus_tag="VLI90_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEPxxWA-CTERM sorting domain-containing protein"
                     /protein_id="HSV15111.1"
                     /translation="MRKLLYAVAGAAALSTASLANAAISIGATSGPIVVGTPDNVTIP
                     NKVNFDTVTNTAGSVSPWFEFSNNQSGMYVFDLTSSTLGTTITLEQLLAGGGTSIIQT
                     VTGSGRFLELQTGTLAADTTYRFLYTFNAPGSGGGTLSGNASFYAAAPGVPEPATWAM
                     MLIGFGGIGFAMRRRRRPILAQVA"
     gene            complement(2008..2259)
                     /locus_tag="VLI90_12710"
     CDS             complement(2008..2259)
                     /locus_tag="VLI90_12710"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HSV15112.1"
                     /translation="MKVAGAWEPEDGGKALVRSFTFKDFSEAFAFLTRVALHAEKIDH
                     HPEFTSVWNRVDFRLTSHDAGGVTERDQKLAEAIDALAQ"
     gene            2464..>3387
                     /gene="hppD"
                     /locus_tag="VLI90_12715"
     CDS             2464..>3387
                     /gene="hppD"
                     /locus_tag="VLI90_12715"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HSV15113.1"
                     /translation="MATQVADQTGILGLDNPMGTDGFEFVEYTAPDIELLRSLFTKMG
                     FPEVARHKSKNVTLHKQGDCNFIINAEAGSYAEEYAKAHGPSACAMAFRVKDAKAAHE
                     RALGLGATNVEVRRGDGELDIPAIEGIGGSRLFFVDRYGDGGSIYDVDFDYHPDWRER
                     MAQADSKLTYIDHLTHNVNRGRMSVWADFYEKLFNFREIRYFDIEGKQTGLFSKAMTS
                     PCGKIRIPLNESQDDKSQIEEFLREYKGEGIQHIALGSSNIYDSVDIIRARGIPFQDT
                     IDTYYDLLDERVKDHGEPLHELKKRKILVDGA"
BASE COUNT          702 a         1038 c          993 g          654 t
ORIGIN      
        1 acagccgagc gcgtcgcgca acgtgcgagg gtcgtgcccg gtgcgatatt cgccactggt
       61 cggctggtcg gccatgaact cgaggctata gcggttggag cgcaatcctc ccccgccttg
      121 tgcctgacgc aggtgatcgc ccgttggtcg aattctcgct tgctaatttt gcaacgcaat
      181 gctggaatcg attgtttacc gatgaaggaa ttcccaacat gtacaaggct ttagttacgt
      241 tgacagttgc agcatcgctt tccggatgcg ctggtacgac ccagaatgca gcgaccgtag
      301 cggaggattg ccgaacggtt gcgcaggaca atacagacag tcacatcaag gtgcaccgcg
      361 attgcagttc cacagccgac gcgccagcac cgtcaacact gggtacgacc gcgagctgac
      421 ccgggattgg cgctgtcctt ccgttctgtc ccactttgaa acaaagaacg gcattcccgg
      481 caaacattcg gcctaacgca tgtacatcaa ctgaagtaac aagttgttaa tcgaaccgtc
      541 caccgacggc gttaacagcg ggagcaagta catgcgtaag gtttttctga gtttggcggc
      601 cgcagccgcg ctgacctgcg cttccgcttc gaatgccgcc atcacagtgg agggtcacag
      661 cagcgacgtg agcgtcggca cacctaataa cagcacgatc cccaacaagg tgaatttcga
      721 ctcggttacg aatacggcgg ctacgatcaa tccatggttc gatttcaaga acgaccagga
      781 tggcttctat gtgttcgatc tgactagcag cacgctctat tcttcgatca ctctggagaa
      841 ggtgctttcg gacggcggca cgacacttgt gaatcaagtc atcgggtccg ggcgttttct
      901 cgagctgatc acccccactc tggacgcggg taccgtctac cgtttcacct acacgtacaa
      961 tacgccggac ggcggcggga ctgtcagcgg aaacgcgagc ttctatcccg cggtgcctga
     1021 gcccgcgact tgggcgatga tgctgatcgg tttcggcggg atcgcgatgg caatgcgtcg
     1081 ccgcggccaa ggcatggcgc tggcgcaagt cgcctaacca acttcgaaca acaattgggg
     1141 cggctcgggg tgacccccgg ggccgccctt tttcacgcgc cgagcagcga tggcctggct
     1201 cggaacccag caactccaat ttaggcgtcc gcacggctaa gtgcctgtat agcagccgaa
     1261 gtgtggcctt ttcgccgcgg tgaatgggta atttccccac gtagaaacgg tctccccgaa
     1321 attgagggaa cctgaatgga taacactccg ttaaccgccc tgtcgccgac gagcggcaca
     1381 acacccctgg aggttccaat gcggaagcta ctctatgctg tcgcgggcgc ggcggctttg
     1441 tctacggcgt cgctcgccaa cgcagcaata tcaatcggcg caacgagcgg gcccatcgtt
     1501 gtcggcacgc ccgacaacgt gacgatcccg aacaaggtca actttgacac ggtgaccaat
     1561 actgccggtt cggtcagccc ctggttcgaa ttctcgaata accagagcgg aatgtacgtc
     1621 ttcgatttga ccagcagcac gctgggcacg acgatcacgc tcgagcaact gctcgcgggc
     1681 ggcggtacgt cgatcatcca gacggtcacc ggatcaggcc ggttcctaga gctccaaacc
     1741 ggcaccctgg cggccgatac cacataccgg ttcctctaca cgttcaacgc gcccggtagt
     1801 ggcggcggta cgctcagcgg caacgccagc ttctatgctg ccgcgcccgg cgttccggaa
     1861 ccggccacct gggcgatgat gctcataggc tttggcggaa tcggcttcgc gatgcgtcgt
     1921 cgtcgccgtc cgatcctggc gcaagtcgcc taagcaacga acgcaacttc cgcgaagggc
     1981 ggtccggtaa tcccgggccg cccttttcta ttgggcgagc gcgtcgatgg cctcggcgag
     2041 cttttggtct cgctcggtca cccctcctgc atcgtggctg gtcagcctga aatcgacgcg
     2101 gttccagacg cttgtgaact cgggatggtg gtcgatcttc tcggcatgca gcgcgaccct
     2161 cgtcaggaaa gcaaaggctt cgctgaaatc cttgaacgtg aagcttcgga cgagcgcctt
     2221 gccaccgtcc tcaggctccc aggcaccagc aaccttcatc gcgtttctcc tgtcctacag
     2281 tgacttggcc gcactaactt agcgccggtg aggtgtattc tcagcatctg caaacgggcg
     2341 ctatccggtc ggtaaggtca cacggaatgc gccgctctcc gtgaccttag tgaccttgag
     2401 gccccggccc atccttgaaa tggcgcgtcc cggggagcag ttggatccca aaggagacag
     2461 ttaatggcca cgcaagtcgc cgaccagacc ggaatcctcg gtctcgacaa tccgatgggc
     2521 accgacgggt tcgagttcgt cgaatatacc gcgccggaca tcgagctcct ccgttcgctg
     2581 ttcactaaga tgggattccc cgaagtcgcg cggcacaaga gcaagaacgt cacgctgcac
     2641 aagcaaggtg actgcaactt catcatcaat gccgaggccg gttcctacgc ggaggaatat
     2701 gcgaaggcgc acggccccag cgcttgcgcg atggcgttcc gggtcaagga cgccaaggct
     2761 gctcatgagc gggcactggg gctcggcgcg accaacgtcg aagtacggcg cggcgatggc
     2821 gagctcgaca tcccggcgat cgaggggatc ggtggatcgc ggctgttctt cgtcgaccgc
     2881 tatggcgatg gtggatcgat ctacgacgtc gacttcgact atcatcccga ctggcgcgag
     2941 cggatggcgc aagcggattc gaagctcacc tatatcgatc acctgaccca taacgtgaat
     3001 cgcggccgga tgagcgtttg ggcggacttc tacgagaagc tcttcaattt ccgcgagatt
     3061 cgctacttcg acatcgaggg gaagcagacc ggcctcttct cgaaggcgat gacctcgccc
     3121 tgcggcaaga tccgcatccc gctcaacgag agccaggacg acaagagcca gatcgaggag
     3181 tttctgcgcg agtataaagg ggagggcatc cagcacatcg cgctcggaag ctcgaacatc
     3241 tacgacagcg tcgacatcat ccgggcccgg ggcattccct tccaggacac gatcgacacc
     3301 tattacgacc tgctcgacga gcgggtgaag gaccacggcg agccgctgca cgaactcaag
     3361 aagcgcaaga tcctggtcga cggcgcc
//
LOCUS       DATIMH010000723         3292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_462682, whole genome shotgun sequence.
ACCESSION   DATIMH010000723 DATIMH010000000
VERSION     DATIMH010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462682"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VLI90_12720"
     CDS             complement(<1..551)
                     /locus_tag="VLI90_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15114.1"
                     /translation="MLERRVLLTGTWAPLAHKLPNGDGAQTMIQLSDGTIMAQGGFDS
                     PSVNWYRLTPVQGSYVNGNWTALAPMHVGRLFYASVMLNTGKVLVLGGEVATDRSETS
                     SAEIYDPSGNSWSTNTASFPEANFGDGMAETLSDGRVLAAAEGSSNCYIYDPFYNTWS
                     AAIPSLNGDAFSEEGWVKLPGGT"
     gene            complement(799..>3292)
                     /locus_tag="VLI90_12725"
     CDS             complement(799..>3292)
                     /locus_tag="VLI90_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV15115.1"
                     /translation="YDRAAEFITAACQVADMPLDQDALLDGYARWLNDVAGAVLAFPA
                     PALLDQSRAVALARKSCELSGYRTGAFVSTLRDALLNNGKSTEADAVLAKGEACCREV
                     IGRQPGDALPLMDLGDLLKLQRRLPEALTAYTQATQLKPDMPQAWAALGECHRLADNL
                     AKMAEDYSQAIALNPNRQWYWHERGYAYMRLGDQRRALPDHTKAIELGDGDAGERVRR
                     AVAYEGLGEFANAEADYARAIEINRASDEVWRAQYEFYQRRGQADRALPALAKAIALV
                     AASPEAQNNLAWALATDSNPAVRDLKTAIAIATKACQSSNYHTAGFINTLVVALTKQV
                     DGFRHQNNDMGGLRQACLAAAEACHEAAPYYEKLTKSRARHEEPWLFAGSCERLSQLL
                     RDMDEPKAAERGYRDAWSIWSKLAASFDIEDDRWHEAANCNLLGDLLGATGRGDEAAE
                     TYRRALSIWERLATETRDPDRRNHLCWTRTALARLFRSQGKTDAADAVFRDAEAACRE
                     AAAMQPTAAEPQLLLGDLLQSQDKFSEAKGAYTEAIRLKPEWPELWAARAECQRRDGK
                     SADVVDDYSRAIALKPDRQWYWHERADAYACLGKWTDALPDHCRAIELGDNDAGERRR
                     RGDCYRALGQFKDAEADYSRAIELDPADGEQWYDRWAIRNQMGETDLAKADLKQAQAW
                     CISAGAEQQNMLAWYLVTSTDLQVRDAPLAVRLAKAAVIARSDWGEAWNTLGVAQYRS
                     GDWAGAIESLNRSMSLRKGGSAEDWYFLAMASHQLGQQTEAAAWYRRAVRSAQVPSGE
                     MRVFKAEADALMPPSVTGQPVTQTPAATQLAH"
BASE COUNT          526 a         1127 c          994 g          645 t
ORIGIN      
        1 atcgtcccac ccggcagctt cacccaacct tcttcgctga acgcatcgcc gttcagcgag
       61 gggatcgccg cgctccaggt gttgtagaac ggatcataga tgtagcagtt gctcgagccc
      121 tccgccgccg cgagcacacg gccgtcggag agcgtttcgg ccatgccgtc gccgaagttg
      181 gcttcgggga aactggcggt gttggttgac cagctgttgc cggagggatc gtaaatttcc
      241 gcggaactcg tctcactcct gtccgtcgca acttcgccgc ccagaaccag cacttttccg
      301 gtgttcagca tgaccgatgc atagaacagc cggccaacgt gcatcggcgc caacgccgtc
      361 cagttgccgt tgacataact gccctgcacg ggcgtgaggc ggtaccaatt gaccgatggg
      421 ctgtcgaacc caccctgggc catgatcgtg ccgtcggaca gttggatcat cgtctgcgcg
      481 ccgtcgccgt tcggcagctt atgggccagc ggcgcccacg tgccggtgag cagcacccgc
      541 cgttccaaca actcgcacgc gtgctggctg catgctcgaa tcctggacga tgcatgtttg
      601 cgcgatgatg cgtgtttcgg cgcccggcga ctccgctttg aacctgccca catgctggct
      661 ccctgatatg gatatccgcg aactgtcgtc tcaaacgtca ggtcgccgcg gcgggtgcca
      721 ggattctttg ggaatgttga cggaccagat gggcgccgga gcgatggtgt caacgcgcgc
      781 gcgttctgta gcagtccttc aatgcgccag ctgcgtggcg gccggggttt gggtcactgg
      841 ttggcccgtg acggacggcg gcatgagcgc atcggcttcg gccttgaaaa cgcgcatttc
      901 accggatggt acctgtgctg accgaacggc gcgacgatac caggcagcgg cctcggtctg
      961 ttggccgagt tgatggctcg ccattgccag gaagtaccaa tcctccgcgc tgccaccctt
     1021 gcgaagcgac atcgagcgat tgagcgactc gatcgccccg gcccagtcgc cgctgcgata
     1081 ctgcgcgacg ccgagcgtgt tccacgcttc gccccagtcg gaccgggcga tgaccgccgc
     1141 cttcgccagt ctcacggcca gcggcgcgtc gcggacctga aggtccgtgc tcgtcaccag
     1201 gtaccacgcc agcatgtttt gctgttccgc gccggccgaa atgcaccacg cctgtgcctg
     1261 tttcaggtcc gctttggcca gatcggtctc gcccatttgg tttcggatgg cccatcgatc
     1321 gtaccactgc tcgccatcgg cggggtcgag ctcgatcgcg cgggagtagt ccgcctcggc
     1381 atccttgaac tgcccgagcg cccgatagca gtcgccgcgc cttcggcgtt ctccggcgtc
     1441 gttgtctccc agttcgatcg cccggcagtg atccggaagc gcatccgtcc acttgccaag
     1501 acaggcatac gcatcggcgc gttcatgcca ataccattgc cggtccggct tgagcgcgat
     1561 cgcccgagaa taatcgtcca ccacatcggc agatttgccg tcgcgccgct ggcactcggc
     1621 cctggccgcc caaagttccg gccattcggg cttcaaccgg atcgcttcgg tatacgcgcc
     1681 cttggcttcg ctgaatttgt cctgcgactg gagcaagtcg cccaggagca gttgcggctc
     1741 ggccgcggtg ggttgcatgg cggccgcctc gcggcaggcg gcctcggcgt cgcggaagac
     1801 cgcgtcggcg gcgtcggtct tgccttgcga tcggaacaac ctcgccagcg cggttcgcgt
     1861 ccagcacagg tgattgcgac ggtccggatc gcgcgtctcc gtcgccagcc gttcccagat
     1921 gctcagagct ctccgatacg tctccgccgc ttcgtcgcct cgaccggtag cgccgagcag
     1981 atcacccagc aagttgcagt tcgccgcctc gtgccagcgg tcgtcttcga tgtcgaacga
     2041 agctgccagc ttgctccaga tcgaccacgc gtcgcgatat ccgcgttcgg cggccttcgg
     2101 ctcgtccatg tcgcgcagca actgcgacaa ccgctcgcac gaaccggcga acagccatgg
     2161 ctcctcatgc cgcgcccgcg acttcgtcaa cttctcgtag tacggcgccg cctcgtgaca
     2221 tgcctcggcg gcggccaaac acgcttggcg aagcccgccc atgtcgttgt tctgatgacg
     2281 aaacccgtca acctgcttcg tcaatgcgac caccagcgtg ttgatgaagc cggcggtgtg
     2341 gtagttcgat gactggcatg ccttggtggc aatggcgatg gccgttttca agtcgcgcac
     2401 ggccggattg ctgtcggtcg ccagcgccca cgccagatta ttctgcgcct ctggtgaggc
     2461 ggcgaccagc gcgatcgcct tggcaagcgc cggcagggcg cggtcggctt gtccccggcg
     2521 ttggtaaaac tcgtattgcg cgcgccacac ctcgtcgctg gcgcggttga tctcaatcgc
     2581 ccgggcgtaa tccgcctcgg cgttggcgaa ttcaccgagc ccttcgtatg ccaccgcgcg
     2641 gcgcacgcgc tcgccggcgt cgccgtcgcc caactcgatc gccttcgtat gatccggcag
     2701 cgcccgccgc tggtccccca gccgcatgta cgcatatccg cgctcgtgcc aataccattg
     2761 cctgttcgga ttcaatgcga tggcctgcga ataatcttcg gccatcttcg ccaggttgtc
     2821 ggccagccga tggcattcac ccaacgccgc ccatgcttga ggcatgtccg gcttcaactg
     2881 cgtcgcctgg gtatacgcag tcaacgcctc tggcagccga cgctgcaact tcagcagatc
     2941 gcccagatcc atcaacggca aagcgtcgcc cggttgccgg ccgatcacct cgcggcagca
     3001 cgcttcgccc ttggccaaaa ccgcgtcagc ctcggtcgat tttccgttat tcaatagtgc
     3061 atcacggagg gtgctgacga acgcgccggt acgatagccg ctcaactcgc acgattttct
     3121 cgccagcgcc accgcgcgcg attggtccaa tagcgcgggc gcgggaaatg ccagcaccgc
     3181 cccggcgaca tcgttcagcc atcgagcgta cccgtcgagc aatgcgtcct gatccagcgg
     3241 catgtcggcc acctgacacg ctgcggtgat gaattcggcg gcacgatcgt at
//
LOCUS       DATIMH010000724         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5375735, whole genome shotgun sequence.
ACCESSION   DATIMH010000724 DATIMH010000000
VERSION     DATIMH010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5375735"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..463
                     /locus_tag="VLI90_12730"
     CDS             101..463
                     /locus_tag="VLI90_12730"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV15116.1"
                     /translation="MSTSTSRKAKQAKNDQQVLVGSTNLFKDLGRPDADEAFAKLELA
                     YKIHSLINKMGLSQTEAARLLNTDRARVSNLMRGRLSEFSIDRLFRFLNKLGQDVEVT
                     IRPKRGARAQVNILAKAG"
     gene            complement(478..1980)
                     /locus_tag="VLI90_12735"
     CDS             complement(478..1980)
                     /locus_tag="VLI90_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15117.1"
                     /translation="MIIRPFVIAFFALATIAIAQTEPSTTGPSTTRSSADEQKVSRSR
                     HEITLDGKKLAYHATAAEMAMKDESGKAKANMFFVAYDLDDARDAATRPITFLFNGGP
                     GAAAVWLHLGAAGPKTIEEGDGDIPVGPPYKLVDNAATWLGSTDLVFIDPVSTGYSRP
                     APGEKAEQFHGVHPDIESVADFIRLYLTKHNRWASPKYLAGESYGTTRAAALSDFLAE
                     RYGVAANGIVLISSVLDFQSLIAGKGNDLPYELYLPSYAAVAWYHKKLSPDLQADFNK
                     TIDAARKFAMEEYAPALQKGSVIAPDQRASIVKQLAAFTGLPAELIDRSNLRIDPGLF
                     EKRLLGDGRQVIGRFDGRITGYDPNAIGNEPGYDPSLSRYLSAYSSTFNDYVRRELKY
                     ESDLPYEVLKNVEPWSFGQEGEGYLNVASKLQDAMVKNPRLRVMFVSGYYDLATPFFA
                     ADYTIDRLELNDELRKNVTHEYFPAGHMVYHHKESARKLAEDIGKFVKPE"
     gene            2141..>2914
                     /locus_tag="VLI90_12740"
     CDS             2141..>2914
                     /locus_tag="VLI90_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15118.1"
                     /translation="MSLAAPAVGAAEAPTTSASQLDIPSLVNKLGDPDPTVRGDASLR
                     LSSLGAAARPALADALHSGAPEVRMQAGQILLRLPWAQPEDPDAVKRVLSGYGELGVD
                     ERCERVSALLEVPNGAGQDAVFRILQDDPSAAVRWAAATALRMTAPGVKPPLERLRAL
                     VVNSDDPAAAAGQPRELPDDAPLLAAVGWAWRETDPQRAGELMSRAVELEALHPSALR
                     GQLDFAFAWLIERATEQQRYADAYHLLRQQAAWTRFDSEG"
BASE COUNT          497 a          875 c          971 g          571 t
ORIGIN      
        1 ttgaaatcac cctcaccccg gccctctccc acgagtacat cggagagggg gaccgagtga
       61 aggctagacg tcgcggaagc acattatcgg gagcacaagc atgagtacct caacgagtcg
      121 aaaggccaag caggcgaaga atgatcaaca ggtgcttgtc ggtagcacca acctgtttaa
      181 ggacctgggt cgtcctgatg ccgatgaggc atttgccaag ttggaactgg cgtacaagat
      241 ccattcgctg atcaacaaga tgggcctgag ccagaccgaa gccgcgcggt tgctcaatac
      301 cgaccgcgcg cgagtttcaa atctgatgcg cggacggctg agcgagttct caattgatcg
      361 gttgttccga ttcctcaaca agctcggcca ggacgttgag gtgacgattc gtcccaaacg
      421 cggtgcgcga gcgcaggtga acatcctcgc caaggccggg tgaccttccg atggcactca
      481 ctccggtttc acgaacttgc ctatatcctc cgccaacttt cgcgccgatt ccttgtggtg
      541 atacaccatg tgccccgcgg ggaaatactc gtgcgttacg ttctttcgca gctcgtcgtt
      601 cagctccagg cggtcgatcg tgtaatccgc ggcgaagaac ggcgtggcca gatcgtagta
      661 tccgctgacg aacatcacgc gcagccgcgg gttcttcacc atcgcgtcct gcagtttgct
      721 cgcgacattc aggtagcctt caccttcctg gccaaagctc cacggctcca cgttcttcag
      781 cacctcgtac ggcaggtcgc tctcatactt cagctcgcga cggacgtagt cgttgaacgt
      841 cgatgaatag gctgacagat aacgcgagag gctcgggtca tagccgggtt cattcccgat
      901 cgcgttggga tcgtaaccgg tgattcgacc gtcgaagcgg ccgatgacct gccggccatc
      961 gccgagcagg cgcttctcga acaggcccgg gtcgatgcgc aggttgctgc ggtcgatcag
     1021 ctccgccgga aggccggtga atgcggcgag ttgcttgacg atcgacgcgc gctgatccgg
     1081 cgcgatcacc gaacctttct gcagcgcggg cgcgtattcc tccatcgcga attttctcgc
     1141 tgcatcgatc gtcttgttga aatccgcctg caggtccggc gatagctttt tgtgatacca
     1201 cgcgacggcg gcgtagctgg gcaagtacag ctcgtacggc aggtcgttgc ctttgccggc
     1261 gatcaggctt tggaagtcga gcacggacga gatgagcacg atgccgttgg cggcgactcc
     1321 gtagcgctcg gcgaggaagt ccgacaacgc ggccgcccgc gtggtgccgt agctttcgcc
     1381 cgcgagatat ttgggcgacg cccagcgatt gtgcttggtc agatacaggc ggatgaagtc
     1441 agcgacgctt tcgatgtcgg ggtgcacgcc gtggaactgc tcggccttct cacccggcgc
     1501 gggccggctg tagccggtgc tgacgggatc gatgaagacc agatcggtcg agccaagcca
     1561 ggtggcggcg ttgtcgacca gcttgtacgg cggaccgacc ggaatatcgc cgtcgccttc
     1621 ttcgatcgtc ttcgggccag ctgcgcccag gtgcagccac accgcggcgg cgccggggcc
     1681 gccgttgaac aggaacgtga tcggccgagt cgcggcatcg cgggcgtcgt ccaagtcgta
     1741 cgcgacgaag aacatgttcg ccttggcctt gccggattcg tccttcatcg ccatctcggc
     1801 ggcggtggcg tgataggcga gcttctttcc atcgagggtg atctcgtgcc gcgagcgcga
     1861 gactttctgt tcatcggcag acgagcgcgt cgtggacggg ccggtcgtcg atggttcggt
     1921 ttgggcgatg gcaatggtcg cgagcgcaaa aaatgcgatg acgaaggggc gaatgatcat
     1981 gtagacctcg gttagcgcgg gcgcagcact cgccgcggct tgcggcttag catccgtttt
     2041 tccggaatat tctcaacagg gctcagcctt gccgcggcga ggtcgcgcga cttaccatat
     2101 gcaaatgaac cggtggacgt tggctccggt cctttttctt ttgtcgctcg cagcgcccgc
     2161 tgtgggcgcg gcggaagcac cgacgacttc tgcgtctcaa ctcgacatcc cttcgctcgt
     2221 caacaagcta ggtgatcccg atccgaccgt gcgcggcgac gcttcgctgc gcctgtcatc
     2281 attgggcgct gcggcgcgcc cggcgttggc cgatgcgttg cacagcggcg cgccggaagt
     2341 ccgcatgcag gcggggcaga tcctgctgcg gttgccctgg gcgcagcccg aggatccgga
     2401 cgcggtgaag cgcgtgcttt ccggttatgg cgagttgggt gtggacgagc gctgcgagcg
     2461 cgtcagcgcg ctgcttgagg tgcccaacgg cgccggccaa gacgcggtgt ttcgaattct
     2521 gcaggatgat cccagcgcgg ccgtgcgatg ggcggcggcg acggcgctgc gcatgacggc
     2581 tcccggtgtg aaaccgccgc tggagcggct gcgggcgctg gtggtgaatt cggatgatcc
     2641 cgcggcggcc gcgggtcagc cgcgcgaact gccggatgac gcgccgctgc tggcggcggt
     2701 gggttgggcg tggcgcgaga ccgacccgca gcgcgccggt gagctgatgt cgcgcgcggt
     2761 ggagttggag gcgctgcatc catcggcact gcgcgggcaa ttggatttcg cctttgcgtg
     2821 gttgatcgag cgcgcgaccg aacagcagcg ctacgccgac gcgtatcact tgctgcggca
     2881 gcaggcggcc tggacgcggt tcgattccga aggc
//
LOCUS       DATIMH010000725         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4504393, whole genome shotgun sequence.
ACCESSION   DATIMH010000725 DATIMH010000000
VERSION     DATIMH010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4504393"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VLI90_12745"
     CDS             <1..950
                     /locus_tag="VLI90_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15119.1"
                     /translation="LISSEENLMRRGPFRLIDLLVGTFIVLFVSGIVLSKAPAANEIK
                     MRVKCASNLRMIGQALLLYSNENRGAFPRTMHDKKDANPKPVWGTPYEANKDLGAVKD
                     ADPFAKDKDAAAKARPAMEDVTAALFLLLRTQDITADVFICPSTQHRKFDFGGGMHTA
                     QDWTNWPGNAGLLANLSYSVNNPYASPAAVKAGFKWNNTMAPDAPVVADMNPGVDGLL
                     KLTLQSPADEMRKCNSINHNQDGQNVLYGDGHVEFQNHPFCGMKRDNLYTYGPTGTDH
                     PNTGGDGIVGGANGEADAVLLPTSTSLGDTNPLLTPEGL"
     gene            complement(1171..2076)
                     /locus_tag="VLI90_12750"
     CDS             complement(1171..2076)
                     /locus_tag="VLI90_12750"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HSV15120.1"
                     /translation="MNQPNRREVLKWSALLAGTSILPRNLFAAADENKSRPRKVLFFT
                     KSSDFEHDVIKRNGEKLGFAERKLIELGKQHDLDITASKDGQLFEPGKLAELDAIVFY
                     TTGDLTTNGKDKNPPMSAEGKQALLDAVRNGVGFVGIHCASDTFHSRSDRELDPYIQM
                     LGGEFIIHGKQQDSTTRVADPKFPGVPDQKDFTLHEEWYALKNFAEDLHVILLQDTTG
                     MVGDMYQRPPYPSTWARRHGNGRVFYTSMGHREDVWTNPIFQNLLLSGVAWSMGAIDA
                     EVTPNLNSVAPGARKIPTLKPTTTR"
     gene            complement(2054..2533)
                     /locus_tag="VLI90_12755"
     CDS             complement(2054..2533)
                     /locus_tag="VLI90_12755"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15121.1"
                     /translation="MPRQILFLCTGNYYRSRFAEIVFNELAKRAGIDYVATSRALAIE
                     LGIFNFGNISTYTRRGLERRSIALPEPVRRPMQCDEADLQCAHRIIALKEAEHRRYLT
                     ERYPAWPDRVKYWHIHDLDQATPEQALAEIERNVIALIEQLRTEDAAGGSRESAESS"
     gene            complement(2698..>2896)
                     /locus_tag="VLI90_12760"
     CDS             complement(2698..>2896)
                     /locus_tag="VLI90_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15122.1"
                     /translation="FQQPLQASFGTERFLHQNTNLADIAHSHRSSPRSKVPVKTLIEA
                     NDGSLYLQIGSYTSHVTIQFV"
BASE COUNT          556 a          846 c          931 g          563 t
ORIGIN      
        1 gtttaatttc ttcagaggag aacctcatgc gccgcggccc attccgcctg atcgatctgc
       61 tcgtcggcac ctttattgtg ctcttcgttt ccggcatcgt gctctcaaaa gcgccggcgg
      121 caaacgagat taagatgcgc gtcaagtgcg catcgaactt gcgcatgatc gggcaggcgc
      181 tgctgctgta ttccaacgaa aaccgcggcg cattcccgcg gacgatgcac gacaagaagg
      241 acgcgaatcc caagccggtg tggggcacgc cgtatgaggc gaacaaggat ctgggcgcgg
      301 taaaggacgc tgatccattt gcgaaggaca aggacgccgc ggccaaggcc cggccggcga
      361 tggaagacgt gacggccgcc ctgttcctgc tgctgcgcac gcaggacatc acggcggacg
      421 ttttcatctg tcccagcacg cagcatcgca agttcgactt cggcggcggc atgcacaccg
      481 cgcaggactg gaccaactgg cccggcaacg cggggctgct ggcgaacctg agctacagcg
      541 tgaacaaccc ctacgcgtcg cccgccgccg tcaaggcggg gttcaagtgg aacaacacga
      601 tggcgcccga cgccccggtc gtcgcggaca tgaaccccgg cgtggatggg ctgctgaagc
      661 tcacgctcca gtccccggca gacgaaatgc ggaagtgtaa tagtatcaat cacaatcaag
      721 acggccagaa cgtcctgtac ggtgacggcc acgtcgagtt ccagaatcac cccttctgcg
      781 gcatgaagcg cgacaacctt tacacctatg gccccaccgg cacggaccac cccaacaccg
      841 gcggcgacgg tatcgtgggc ggcgcgaacg gcgaagcgga cgcggtgctg ttgccgacat
      901 ccacgagcct cggcgacacc aacccgctgc tgacgcccga ggggctgtaa gttgacggct
      961 cgacattttc agaagtggca gcaaccaacc acgccgggtt cggaggtact ccgacgaagc
     1021 gagattttgc cgcgcgcgag gagaagaatc cgggtcgtcg gagtacctcc gaacccggcg
     1081 tgaggaggtt cgacgccccg tggatctgtg gtcgggaagg gctggggcgc gcgtgcgcgg
     1141 gtatttcggc gggcgacatc ctgccaacga tcacctcgtc gtcgtcggct tcagcgtcgg
     1201 aatcttgcgc gcgccgggcg cgacgctatt caggtttggc gtgacctccg cgtcaatcgc
     1261 gcccatgctc cacgcgacgc cgctcagcag caggttttgg aagatcgggt tcgtccacac
     1321 gtcttcgcgg tggcccatcg acgtgtagaa cacccgcccg ttgccgtggc ggcgggccca
     1381 ggtgctggga tacggcgggc gctggtacat gtcgccgacc atgccggtgg tgtcttgcag
     1441 caggatgacg tgcaggtctt cggcgaagtt tttcagcgcg taccactctt cgtgcagcgt
     1501 gaaatctttt tgatcgggca cgccggggaa ctttggatcg gcgacgcgcg tcgtcgagtc
     1561 ctgttgtttg ccgtggatga tgaattcgcc accgagcatt tggatgtaag gatcgagttc
     1621 gcgatcggat cggctgtgga acgtgtcgct ggcgcagtgg atgccaacga agccgacgcc
     1681 gttgcgcacc gcgtcgagca acgcctgctt tccttcggcc gacatcggcg ggttcttgtc
     1741 cttgccgttc gtggtgagat cgccggtggt gtagaagacg atcgcgtcca gctccgcgag
     1801 cttgccgggc tcgaacagtt ggccgtcttt gctggcagtg atgtccaggt cgtgctgctt
     1861 gcccagctcg atcagctttc gctcggcgaa gccgagtttt tcaccattgc gcttgataac
     1921 gtcatgctcg aagtcggatg acttggtgaa gaacagcacc ttgcgcgggc gcgatttatt
     1981 ttcatccgcc gcggcgaaca gattcctcgg cagaatgctc gtccccgcaa gcagcgccga
     2041 ccacttcaac acctcacgac gattcggctg attcacgcga gcctccggct gcatcctcgg
     2101 ttcgcaactg ctcgatgagc gcgatgacgt tgcgctcgat ctcggccagc gcctgctccg
     2161 gcgtggcctg atcgagatcg tggatgtgcc aatactttac gcgatccggc cacgccgggt
     2221 aacgctcggt cagatagcgg cgatgctccg cttccttcag cgcgatgatg cgatgggcgc
     2281 actgcaggtc cgcctcatcg cactgcatcg gccggcgcac cggttccggc agcgcgatgc
     2341 tccggcgttc cagcccgcgg cgggtgtagg tggagatatt gccaaagttg aagatgccca
     2401 gctcgatggc gagggcacgc gatgtggcga cgtagtcaat ccccgctcgc ttggcgagtt
     2461 cattgaaaac gatctcggca aatcggctgc ggtaataatt gccggtgcag agaaagagaa
     2521 tctggcgggg catcattttc atgtagcacg ggcatctccc ccgtgtcgag ggggtcgaat
     2581 gcgaacacga agtcgcgaag gcgcgaaggc gcgaagaaag acgcgaagat gacttggaga
     2641 aagaggagtg ttttgcgttc gctcgcacgg gccggaggcc gtgtcacggt ggtgttgtca
     2701 aacgaattga atcgtgacat gggaagtgta acttcctatc tgtaaataaa gtgagccatc
     2761 gttcgcttcg atcagggttt ttaccggaac ctttgatcga ggagacgaac gatggctgtg
     2821 agcaatatcg gcgagattgg tattttggtg caagaagcgt tcagtgccga aggaggcctg
     2881 aagcggctgc tggaag
//
LOCUS       DATIMH010000726         1925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4215632, whole genome shotgun sequence.
ACCESSION   DATIMH010000726 DATIMH010000000
VERSION     DATIMH010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4215632"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VLI90_12765"
     CDS             complement(<1..399)
                     /locus_tag="VLI90_12765"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HSV15123.1"
                     /translation="MFSLDAGAAVGLRRNIELKARCRDLWSARAAAMGELTATEAGRF
                     EQLDTYFHCHHGRLKLRETAGAASAELIWYDRSNDAKVRPSDYRLASIANPAELKAAL
                     TDALGVRGEVRKRRQLLLWHNVRIHLDEVEG"
     gene            complement(485..649)
                     /locus_tag="VLI90_12770"
     CDS             complement(485..649)
                     /locus_tag="VLI90_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15124.1"
                     /translation="MIALLSLSFVSPLLVVGCDSNSEKTTTHSRNPITGTEKTTTESQ
                     SHAHANDNNP"
     gene            865..1212
                     /locus_tag="VLI90_12775"
     CDS             865..1212
                     /locus_tag="VLI90_12775"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15125.1"
                     /translation="MEATTATINYDDFAKLDLRVATVVECKPHANADKLLVLQIELAG
                     GERRQICAGLRAFYQPEQLVGKQIVVVANLAPRTMRGEISQGMLLAASDPVTSKVIVV
                     TPSEPAAPGSKVS"
     gene            1254..>1925
                     /locus_tag="VLI90_12780"
     CDS             1254..>1925
                     /locus_tag="VLI90_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSV15126.1"
                     /translation="MLRELHISNLAVISDARIELAPGLNCFTGATGAGKSLVIGALEV
                     LLGLRSPAEMLRPGAEEGRVSGVFEVAGKDVLARIQSATDVNVSADGGEILLTRKLYA
                     SGRSSVSLNGQPITLSMLKQVSEHLVDVHGQHDHQYLLKPANQLDVIDQFGDLSSLRQ
                     RYYAVYEQLQSARRRLEELATNKLLRQQQLDLYRFQAQEIDSAELDAAEYAELASRSS
                     VLVNLE"
BASE COUNT          355 a          558 c          637 g          375 t
ORIGIN      
        1 gccttccact tcgtccaggt gaattcgcac gttgtgccac agcaacagct gccggcgttt
       61 gcggacttcc ccgcgtacgc cgagcgcgtc ggtcagtgcg gctttcagtt ctgcgggatt
      121 ggcaatgctg gcaagccgat aatcgctcgg ccgcactttc gcgtcgttgc tgcggtcgta
      181 ccagatcaat tccgcactgg cggcgcccgc ggtctcgcgc aacttcagtc ggccgtgatg
      241 gcagtggaag taggtgtcga gctgttcgaa ccgaccggct tcggtcgccg tcaattcccc
      301 catcgccgcc gcgcgcgcag accacaagtc gcggcagcgc gctttcaact cgatatttct
      361 tcgcaagccc accgccgcgc ccgcatccaa tgaaaacacc gcggcggttt tacccgccgc
      421 ggtgcggtgt cacaacgttc gtcgcttgca tggaggcaag cgcgcactcg ccgacgccac
      481 caaatcacgg gttgttgtca ttcgcgtggg cgtgcgactg cgattcggtc gtcgtcttct
      541 ccgtacccgt gatcgggttg cgggaatgag tggtggtctt ttcggagttg ctgtcgcaac
      601 cgacgaccag cagcggggaa acgaagctca gcgagagcaa agcgatcatg atgtagcgca
      661 tagttggctc ctttcagcca tgggttctga cagttttcac ctttaatccc gttagcagcc
      721 tcatcgcaaa tgccgggcca cgtgcaccgg gggttgcaac ttgatgcgga gatgaatgcg
      781 ggcggtcgag cttggcgtag cgatttccgc tgcgtacact cttgcaacta ttatagtaac
      841 gattgcaggc tcaacgggtg cgacatggaa gctacgacag cgacaataaa ctacgacgat
      901 tttgccaagc tggacttgcg ggtggcgacg gtggtggaat gcaagcccca cgcgaacgcc
      961 gacaagctgc tggtgttgca gatcgagttg gccggcgggg aacgccggca gatttgtgcc
     1021 gggttgcgcg cgttctatca accggagcag ctggtgggca agcagatcgt cgtggtggcg
     1081 aacctggccc cacggacgat gcgcggggaa atcagccagg gcatgctgct ggcggcaagt
     1141 gacccggtga cgagcaaggt gatcgtagtg acgccaagcg aaccggcggc gccggggtcg
     1201 aaagtgtcgt agcgacgggt gcgttgttgt tcgggccgtg cacggacgcg ctgatgcttc
     1261 gcgaactcca tatctccaac ctggcggtca tctccgatgc gcgcattgaa ctggcgccgg
     1321 ggctgaattg ctttaccggc gccaccggcg cgggcaagag cctggtcatc ggcgcgctgg
     1381 aagtgctgct tggcctgcgc agccccgcgg agatgctgcg gccgggcgcc gaagaaggcc
     1441 gggtgagcgg cgtgtttgaa gtcgccggta aagatgtgct ggcgcgcatt cagtccgcga
     1501 cggacgtgaa cgtcagcgct gacggcggcg agatcctgct gacgcgcaag ctgtacgcca
     1561 gcgggcgaag ctctgtctcg ctcaacggcc agccgatcac gctcagcatg ttgaagcagg
     1621 tgtcggaaca cctggttgac gtgcacggac agcacgatca ccagtacctg ctgaagccgg
     1681 cgaatcagct ggatgtgatc gatcagtttg gcgatctgtc atcgctccgg cagcgctatt
     1741 acgcggtgta cgaacagctg cagtcggcgc gacgccgatt ggaagaactg gcgacgaaca
     1801 aattgttgcg gcagcagcaa cttgacctgt atcgcttcca ggcgcaggag atcgactcgg
     1861 ccgagttgga cgcggcggag tacgcggagc tggcgtcgcg atcgtcggtg ctggtcaatc
     1921 tcgag
//
LOCUS       DATIMH010000727         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5376044, whole genome shotgun sequence.
ACCESSION   DATIMH010000727 DATIMH010000000
VERSION     DATIMH010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5376044"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VLI90_12785"
     CDS             complement(<1..680)
                     /locus_tag="VLI90_12785"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSV15127.1"
                     /translation="MRHFDCAVIGTGPAGQKAAIQAAKLGKSVVIIEKKNVLGGAAIN
                     TGTIPSKALREAVLHLTGVSKRGLFGESYKVKRNITIADLIYISQQVIHNELDLIRNH
                     LDRNNVELVWGAARFDSEVSLYVDRPHDFEMISADVFIIASGTRPARPESVPFDDKTV
                     FTSDELMNLDHLPKSMIIVGGGVIGVEYACMMATLGVKVTLVEGRREVLGFLDSEIAE
                     AFQYQMRR"
     gene            complement(717..1346)
                     /locus_tag="VLI90_12790"
     CDS             complement(717..1346)
                     /locus_tag="VLI90_12790"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HSV15128.1"
                     /translation="MPEESAIVTYALQPDDYVALSEFAQGKATRDNRWLARFSVYIGS
                     VAATILSLAVSDAVSARSITVAAIMTAVFVLLYTSWLWPHPALTLRKQRARYYQSPKL
                     LEQRMLQIDGTGVRQHSDSGESFTRWNTIERVVVTAEYLFIFTSSIGGYIIPRRAFAT
                     AAEFLSFAAVAQRHWRPEPDASRTGGFPVVQKSSTAAETVGLETPSTDD"
     gene            complement(1387..>2877)
                     /locus_tag="VLI90_12795"
     CDS             complement(1387..>2877)
                     /locus_tag="VLI90_12795"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HSV15129.1"
                     /translation="PAQSPAAPPRGALGHAPPLTLGDLSEAFEDLSRIGNAERKRELL
                     HALFTRCAHPREAAYLAKIIFRDLRTGVQEGVLQAAIAQAFGRPLAAVQRCQLLVGDL
                     DEVAVLAKHDALATPKFRLFHPIQFMLATPQETAAIAADTLAGRMFYAEDKLDGIRAQ
                     VHKQGDRIAIYTRTMDRTDDSFPDVVETIRHLGGDFLLDGEIVPYRDGVVLPFAHIQK
                     RLGRKVLPAKILRDNPAAFIAFDILYRDGALLMDRPLRERRAALLSLAQVNDASPHAS
                     GVDAMTSKDAPRLLVSAATDVRTAIDIEAAFNKSRESRNEGLVLKDPDSIYSPGRRGK
                     MWLKLKTYLPTFDCVVTAAEYGHGKRRNSLSDYTFAVWDREPSEDGATLVNVGKAYSG
                     VTDEEIAQLTKLFLELSRGQWGRVHYVEPKVVLEIACDQIQKSARHQSGYALRFPRIK
                     RVRWDKRPEEADRLARIVEIYESTHNFGRGSIDSTPAPEPSLFDKC"
BASE COUNT          540 a          928 c          843 g          566 t
ORIGIN      
        1 atccgccgca tctgatattg aaacgcctcg gcgatttcgc tgtcgagaaa cccgagcact
       61 tcgcggcggc cttcaacgag cgtcaccttc acgccaagcg tcgccatcat gcacgcgtat
      121 tccaccccga tcacaccacc gccgacgatg atcatcgact tgggcagatg atcgaggttc
      181 atcagctcgt ctgatgtgaa gaccgtcttg tcatcgaacg gcaccgactc cggccgcgcc
      241 ggtcgcgtgc cgctggcgat gatgaacacg tccgcgctga tcatctcaaa gtcgtgcggc
      301 cgatcgacgt acagcgacac ttcgctgtcg aaccgcgccg cgccccatac cagttcaacg
      361 ttgtttcgat cgagatgatt gcggatcagg tccagctcat tgtggatgac ctgctggctg
      421 atgtagatca gatcggcgat cgtgatgttg cgtttgacct tgtacgattc gccgaacaat
      481 ccgcgcttgc tgacgccggt cagatgcagc accgcttcgc gcagcgcttt ggatggaatg
      541 gtgccagtgt tgatggccgc cccgcccagc acattcttct tctcgatgat aacgacggat
      601 ttgcccagct tagcggcctg aatagccgcc ttctgccccg ccgggccggt gccgatgacc
      661 gcacaatcga aatgtctcat gatggtccga aaacgggcaa ttggttgcgc aggatgttag
      721 tcgtcggtgg atggtgtttc caatccgacg gtttccgcag ctgtcgagct tttctggacg
      781 accggaaatc cgcccgtgcg gctggcatcg ggttcaggtc tccagtgtcg ctgtgccacc
      841 gctgcgaacg ataggaattc agccgcggtg gcaaatgcgc gcctcggaat gatgtagccg
      901 ccaatcgagc tggtgaaaat gaaaagatat tcagctgtga ccacgacccg ttcgatggtg
      961 ttccaccgag tgaagctctc gccggaatct gagtgctgac gaacgcccgt tccatcaatt
     1021 tgaagcatcc gctgttccag cagcttcggg ctttgatagt aacgcgcgcg ctgctttcgc
     1081 agtgtcagcg cagggtgtgg ccagagccat gaggtgtaga gcaatacaaa aacagcagtc
     1141 atgattgctg cgacggtgat agaacgcgca gacacagcat cggaaacggc caagctcaag
     1201 atggttgcag cgaccgagcc gatgtagacg gaaaaccgcg ctaaccagcg attgtcacgc
     1261 gtcgctttac cttgggcgaa ttccgacagc gcgacatagt cgtccggttg caaggcatac
     1321 gttacgatgg cgctctcctc gggcatggat ttacagtagt tgcttataag tccaaccgtc
     1381 caaccgtcaa cacttatcaa atagagacgg ctctggtgca ggcgtggaat cgatgctgcc
     1441 tcgcccgaaa ttgtgcgtgc tctcgtatat ctccacgatc cgcgccagcc gatccgcctc
     1501 ctccggccgc ttgtcccagc gcacccgctt gatgcgcgga aagcgcagcg cgtagccgct
     1561 ctggtgccgc gccgatttct ggatctgatc gcacgcgatc tccagcacga ccttcggctc
     1621 cacgtaatgc acccgccccc actgcccgcg ggacagctcc aggaacagct tggtcagctg
     1681 cgcaatctct tcatccgtca ccccgctgta cgccttgccg acgttcacca gcgtcgcgcc
     1741 gtcttcgctc ggctcgcggt cccacaccgc gaacgtgtaa tcgctcagcg aattccgccg
     1801 cttgccgtgg ccgtactccg cggcggtcac gacgcagtcg aatgtcggca aataggtctt
     1861 gagcttcaac cacatcttgc cccgccggcc aggcgaatag atcgaatccg ggtccttcag
     1921 cacgaggcct tcgttgcggc tctcgcgcga cttgttgaac gcggcttcga tgtcgattgc
     1981 cgtgcgcacg tccgtcgccg ccgacaccaa cagacgcggc gcgtctttcg acgtcatcgc
     2041 atcaacgccg ctcgcgtgcg gagacgcgtc gttcacctgc gccagcgaca gcagcgcggc
     2101 acgtcgctcg cgcaaagggc ggtccatcag caacgcgcca tcgcgataca gaatgtcgaa
     2161 cgcgatgaac gccgcggggt tgtcgcgcag aattttggcc ggcaggacct tccgcccgag
     2221 ccgcttctgg atgtgcgcga acggcagcac gacgccgtcg cggtacggca cgatctcgcc
     2281 gtcgagcaaa aagtcaccgc ccaagtgccg gatcgtctcg acgacatcgg gaaaactgtc
     2341 atcggtgcgg tccatcgtgc gcgtgtagat cgcaatgcga tcgccttgct tgtgcacctg
     2401 cgcgcgaatg ccgtcgagct tgtcctcggc gtaaaacatc cgccccgcca gcgtgtcggc
     2461 cgcgatcgcc gccgtctcct gcggcgtggc cagcatgaac tggattgggt ggaacaaccg
     2521 gaacttcggc gtcgccagcg catcgtgctt cgccagcacc gcgacttcat ccagatcacc
     2581 cacgagcagt tggcagcgct gcaccgctgc cagcggccga ccgaacgcct gcgcgatcgc
     2641 cgcctgcagc acgccttcct gcacaccggt tcgcaagtct cggaagatga tcttggcgag
     2701 gtacgccgcc tcgcgcgggt gggcgcagcg cgtgaacagc gcgtgcagca gctcgcgctt
     2761 gcgctcggcg ttgccgatgc gcgatagatc ttcgaacgct tcagacagat ctccaagggt
     2821 gagcggcggc gcatgtccga gcgcgccccg cggcggagcc gctgggctct gtgctgg
//
LOCUS       DATIMH010000728         3474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5087416, whole genome shotgun sequence.
ACCESSION   DATIMH010000728 DATIMH010000000
VERSION     DATIMH010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5087416"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..950)
                     /locus_tag="VLI90_12800"
     CDS             complement(135..950)
                     /locus_tag="VLI90_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HSV15130.1"
                     /translation="MSSSAAITPARRPKQATRIGSVSYLNAKPLIYGLDEADDLQLTL
                     DVPSKLIDGLRERRFDVALLPVIDYQRLPGLRILTSGGIGSDGPTLTVRIFSKVPIEQ
                     ITRLACDTDSHTSVALARIILAESFNLHARFIDWNHEERTPAEARLLIGDKVVCEEPP
                     GFDHQLDLGEAWKRLTGMPFVFAAWMAREGVELGDLPQRLEDAKREGLQHAVELVERF
                     AVPRGWPAELALQYLTVYLKYDIGDRQLVAIRKFYELATKHQIIAHPPAELTV"
     gene            complement(1016..2005)
                     /locus_tag="VLI90_12805"
     CDS             complement(1016..2005)
                     /locus_tag="VLI90_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15131.1"
                     /translation="MRFINAVHRRLVVIRAGETAGICLLVACALLAPLLMILWWNGEP
                     AVPATLLGIISGLWLGVSIALTVRRWPSTMMAASEADRQFELADLLATAMVLDASYAS
                     GTSDLMSGEVLAVADARCRTLSPSQLVLRRLGSRTWGGVGLSLALVITLALVPTRPAR
                     LDAAETANDATAANALTTPTPASISSTTAPNRHPSRVDPANEDSTMMTSAEDNATGNQ
                     PTVAARSNSTHAAGSGGGESQSNRPAATPDLPTVAGQHDAKASGEAAGGSSRHGSNGG
                     DNIRSGSATAHSRATPQWHGGESGTSPNDGSLAAPDRVPGEYRDLVRDYFQRD"
     gene            2166..>3474
                     /locus_tag="VLI90_12810"
     CDS             2166..>3474
                     /locus_tag="VLI90_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSV15132.1"
                     /translation="MFRKSNRRRMLRVLVPLTVMAGAARAADSTTSSINHAKPTTVPE
                     SYWPAPMTQEPALKTSPPVGPFEPTWDSIRPNYKVPSWFKGAKFGLFIHWGLYAVPAY
                     HNEWYARHMYNAFAKYHTETYGPPDQFGYKDFIPMFKAAKFNADEWAEIFKKSGARYV
                     IPCAEHHDGFAMWDSDITPWCAGTLGPKRDFIGELAVAIKKQGLVFGVSSHRMEHHAF
                     EFPAKGLKTDVFDPRYKDFYGPPIDNPKLWTGPGASPEFQEDWFRRCAELVDKYDPQM
                     VWFDNGVNAREYDPIKLKFAAYYYNRAHERGHEVTISTKDSAYLAGSIMDFEKIGARS
                     PKDIRPGEWQVDDPIGSTWGYTSDERFTTTAAIPGKLVDTVSKGGTYLLNLSPNADGV
                     IVDPQPQRLAELGQWLATNGEAIYDTEPWTKFSDGGRSPIRFTT"
BASE COUNT          659 a         1094 c         1093 g          628 t
ORIGIN      
        1 gcggcccgcg gccgttcggc gatatggaaa gcgacgcggg cgttcgcttg cctaccgatt
       61 actggtacgc gctgggccgg gatgaagcgc tggaaaaaga tctggcaacc gccatcgagc
      121 ggcgattgca atgatcacac cgtcagctct gccggcggat gggcgatgat ctggtgcttc
      181 gtcgccaatt catagaactt gcgaatcgcc accagctgcc gatcgccgat gtcatacttc
      241 agatagaccg tcaggtactg cagcgccagc tccgccggcc aaccgcgcgg cacggcgaag
      301 cgttccacca attcgaccgc gtgctgcagg ccttcgcgct tcgcgtcttc gaggcgctgt
      361 ggcagatcgc ccagctcgac gccctcgcgc gccatccacg cggcgaagac gaacggcatt
      421 cccgttagcc gcttccacgc ttcgcctaga tccagctgat ggtcgaaacc cggcggttcc
      481 tcgcacacca ccttgtcgcc gatcaacagc cgggcctcgg cgggcgtgcg ctcctcgtgg
      541 ttccagtcga tgaaccgcgc atgcaggttg aacgactcgg ccaggatgat gcgcgccagc
      601 gcgaccgacg tgtggctgtc ggtgtcgcac gccaggcggg tgatctgctc gatcggcacc
      661 ttactgaaaa tgcggacggt gagggtcggc ccgtcgctgc cgatcccgcc gctggtgagg
      721 atgcgcaggc caggcaaacg ctggtaatcg attaccggca gcagcgcgac atcaaaccgg
      781 cgttcgcgca atccgtcgat caatttcgac ggcacgtcga gcgtcagttg aagatcgtcg
      841 gcttcgtcga gcccataaat caacggcttg gcgttgaggt aactcacgct gccgatgcgc
      901 gtcgcctgct tcggccggcg cgcgggcgtg atggcggcgg atgaactcat gccgaagttt
      961 atagccgccg atacggcggc gtcacagttt cagacgtttt gaaaaagcgg gaagatcagt
     1021 cgcgctgaaa atagtcgcga accagatcgc gatattcgcc gggcacgcga tcgggagccg
     1081 cgagcgagcc gtcattcggt gatgtgcctg attctccgcc gtgccactgc ggcgtcgcgc
     1141 gcgagtgcgc ggtggcggag cctgaacgga tattgtctcc gccgttgctg ccgtgacggc
     1201 ttgacccacc cgcggcttcg ccggatgcct tggcatcgtg ctgccccgcg acggtcggca
     1261 agtccggcgt ggcagcgggt cgattggact ggctctcgcc gccaccgctc cctgccgcat
     1321 gtgttgagtt cgaacgagcg gccaccgtgg gttggttgcc ggtcgcgttg tcttccgcgg
     1381 acgtcatcat ggtcgaatct tcgttggccg gatcgacacg cgatgggtgt cgattcggtg
     1441 cagtcgtcga cgagatcgac gccggcgtcg gcgtggtcag tgcgttcgcc gccgtcgcat
     1501 cgttcgctgt ttcggcagcg tccaatcgcg ctggccgagt aggaaccagc gccagagtga
     1561 tgacgagcgc gagtgataag ccgaccccgc cccaggttcg gctgccgaga cgacgcagca
     1621 ccagttgcga tggcgacaac gttcggcacc gcgcatccgc gaccgccagc acttcgccgc
     1681 tcatcagatc ggatgtgccg ctcgcatagc ttgcatcgag caccattgcc gtcgccagca
     1741 aatcggccag ttcgaattgc cgatcggctt cgctcgcagc catcatcgtg ctgggccaac
     1801 gccgaaccgt cagcgcgatg gataccccga gccaaaggcc cgagatgatt ccaagcaacg
     1861 ttgccggcac ggccggctct ccattccacc acaagatcat cagtaacggg gcgagaagcg
     1921 cgcacgccac cagcaggcaa atgcccgcgg tttcaccagc gcggatcacc accagccgcc
     1981 gatgaacggc gttgatgaat cgcatgtgaa ctgaagcggt gctcgccacg acccgattat
     2041 acacgtccca tcgcccgctc cggccgagct attggaatta tgtcacatac gcaaaacacc
     2101 gcggcggggt gcgtggtatg tcagaggaac agcgatgagc actccgagtt tataatgagg
     2161 ttcgcatgtt cagaaaatcc aaccgccgtc ggatgcttcg cgtgcttgtg ccgctgaccg
     2221 tgatggcggg cgcggcgcga gccgctgatt cgaccacgtc atcgatcaat catgcgaagc
     2281 ccacgaccgt gcccgagtcg tattggccgg cgccgatgac gcaggagccc gcgttgaaga
     2341 cgtcgccgcc ggtggggccg tttgagccga cgtgggattc gatccgcccc aactacaagg
     2401 tgccatcctg gttcaagggc gcgaagttcg gcctgttcat ccactggggt ctctacgcgg
     2461 tgccggcgta tcacaatgag tggtacgcgc ggcacatgta caacgcgttc gccaagtacc
     2521 acaccgaaac ctacggtccg ccggatcagt tcgggtacaa ggatttcatt ccgatgttca
     2581 aggccgcgaa gttcaacgcc gacgagtggg cggagatttt caaaaagtcc ggcgcgcggt
     2641 acgtcatccc gtgcgcggaa caccacgatg gttttgcgat gtgggactcg gacattaccc
     2701 cgtggtgcgc gggcacgctc gggccgaagc gcgacttcat cggcgagctg gccgtcgcca
     2761 tcaaaaagca ggggctggtg ttcggcgtgt cgagccaccg catggaacat cacgcgttcg
     2821 aattcccggc caagggtttg aagactgacg tgttcgaccc gcgctacaag gatttttacg
     2881 gccccccgat cgacaacccg aaactgtgga ccggccccgg cgcgtcgccg gagtttcagg
     2941 aagactggtt ccgccgctgc gccgaattgg tcgacaagta tgacccgcag atggtctggt
     3001 tcgacaatgg cgtgaacgcg cgcgagtacg atccgatcaa gctgaagttc gccgcctatt
     3061 actacaatcg cgctcacgaa cgcgggcacg aggtcaccat ctcgacgaag gactcagcgt
     3121 atctcgccgg gtcgatcatg gatttcgaaa agatcggcgc gcgatcgccc aaggacattc
     3181 gccccggcga gtggcaggtg gatgatccga tcggttccac gtggggttac accagcgacg
     3241 aaaggtttac cacgacggcg gccatccccg gcaaactggt ggacaccgtc tccaaaggcg
     3301 ggacgtactt gctgaacctc tcgccgaatg ccgacggcgt catcgtcgat ccgcagccgc
     3361 agcggctggc ggaactgggg cagtggctcg cgacgaatgg agaagcgatc tacgacaccg
     3421 agccgtggac gaaattctcc gatggcgggc gttcgcccat ccgcttcacg acca
//
LOCUS       DATIMH010000729         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1333743, whole genome shotgun sequence.
ACCESSION   DATIMH010000729 DATIMH010000000
VERSION     DATIMH010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333743"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VLI90_12815"
     CDS             <1..326
                     /locus_tag="VLI90_12815"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSV15133.1"
                     /translation="GQDQGRLGLALGPITPDVAGQLSLPDGTQGAVVTEVQPGSAAEQ
                     AGIQAGDVIVGVGTRPVNNPSQAANAIRSAERSGESAIALRIIHDGQSAFVAVNVGGN
                     NANNG"
     gene            441..1886
                     /locus_tag="VLI90_12820"
     CDS             441..1886
                     /locus_tag="VLI90_12820"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HSV15134.1"
                     /translation="MKDGHQHLQSLRDGRDVRLNGARVADPTEHRAFRNACRTAAGLY
                     DFQCQPNRQALMTFETETGQRVNRCWQLPTSYQELLQRRRALEAWAETHLGFFGRSPD
                     HVASTLSGFMMGIEVFEEYDRQRADALREYHAYARDHDLYLTYVIINPQADRGRAAHD
                     QQDAFLTAGVVDTDATGITVRGAKMLATAAVMANEVFVSSIQPLAPGDERYAISFALP
                     LNAAGLRILSRKSYEEAAVSRFDNPLSSRFDENDALLFFDDVRVPWERVFVNQDLAMC
                     QRQFHATPAHVYQNYQAQVRLMVKLRFLAGLAHRIADTNGTLGFPQVREVLGQIAANV
                     GLVEGLVWGMEARGGMAGRYYVPDRNLLYTAQVVTQQMHPQMMTLIRELAGGGMIMLP
                     SSVADFADAELASLIRRSQSSPFYGAADRVKFFKLAWDAVGSEFGSRHTQYEMFYAGA
                     TFVTKAHAFRTYDWKRSTAMVDGVMDGIDPV"
     gene            2140..2481
                     /locus_tag="VLI90_12825"
     CDS             2140..2481
                     /locus_tag="VLI90_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15135.1"
                     /translation="MLGYPRLRDRGDSMSGRCFDYPLPKGFAFDIPELEMLQRWAEGQ
                     AMRMVVELDHCVEGEEYEEVLAFYEDGSALRRWTMWRAAGYFVLEPMNGPDWRFAELP
                     DLIAEMVALRS"
     gene            complement(2476..2880)
                     /locus_tag="VLI90_12830"
     CDS             complement(2476..2880)
                     /locus_tag="VLI90_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15136.1"
                     /translation="MAVFDLTIALLVIAALLARLARWLQVPYPSLLALARTAVAFVPG
                     APRAALDPTLALALFVAPTLLDAAFDASPRDPSAALRRVVARQRNVLGDLRRSGRIGD
                     DAFHAVEEEIDLLELSADPRLRPPRRVGPAPS"
     gene            3109..>3554
                     /locus_tag="VLI90_12835"
     CDS             3109..>3554
                     /locus_tag="VLI90_12835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HSV15137.1"
                     /translation="MDFGAAMFFTDYSMAPDELARALEERRFESVWAPEHSHIPLSRQ
                     SPFPGGGDLPKRYYDTMDPFVTLTAAAAATKRLRIGTGVALVVQRDPIQTAKLVASID
                     QISGGRFLFGVGNGWNAEEMADHGTKDFKSRHKLVRERIEAMKAIWT"
BASE COUNT          624 a         1027 c         1232 g          671 t
ORIGIN      
        1 atggacagga ccagggccgc ctcggtttgg cgcttggccc gatcacgcct gatgttgccg
       61 gccagctcag tctgccggac ggcacgcagg gcgcggtggt gacagaagtg cagccgggtt
      121 ccgcggctga acaggccggc attcaggcag gcgacgtgat cgtcggggtc ggcacacggc
      181 cggtcaacaa tccgtcgcag gctgcgaatg ccattcgcag cgccgagcgc agcggtgaaa
      241 gcgccattgc gcttcgcatc atccacgacg gccagtcggc attcgtggcg gtgaatgttg
      301 gcgggaacaa cgccaataac ggctgaactg gaggcttgat ccaggggccc ggctgcgttc
      361 cacgcagtcg ggccctgatc gggctctgac gcggcatctt tgttgtcgta acataccccg
      421 cgacgcggag ggagacggca atgaaagatg gccaccagca tctgcagtcc ttgcgcgatg
      481 ggcgcgatgt ccgcctgaac ggagcccgcg tcgccgatcc gaccgagcac cgcgcgtttc
      541 gcaacgcctg ccgcaccgcc gccgggttgt acgatttcca gtgccagccg aaccggcaag
      601 cgctgatgac attcgaaacg gaaaccggac aacgcgtcaa tcggtgctgg cagcttccga
      661 ccagttatca ggaactgcta cagcgccgcc gtgcgctgga ggcctgggcc gagacgcatc
      721 tggggttctt cggccggtcg ccggatcatg tggcttcaac gctctcgggg ttcatgatgg
      781 gcatcgaggt gttcgaggaa tacgaccgtc agcgtgccga tgcgttgcgc gagtaccacg
      841 cctatgcgcg tgaccacgat ctgtatctga cctacgtcat catcaacccg caggcggatc
      901 gcggccgcgc ggctcatgac cagcaggatg cgtttctaac cgcgggggtg gtcgatacgg
      961 atgcgaccgg gatcacggtg cgtggcgcga agatgctggc taccgcggcg gtgatggcga
     1021 acgaagtctt cgtatcctcg attcagccac ttgcaccggg cgatgagcgc tacgcgatat
     1081 cgttcgcctt gccgttgaat gcggcgggac tgcgcattct gtcacgcaag tcgtatgagg
     1141 aagcggcggt ttcgcgcttc gacaacccgc tgtcgagccg gttcgacgag aacgatgcgc
     1201 tgctgttttt cgatgacgtt cgggtgccgt gggagcgcgt gttcgtcaat caggatctgg
     1261 caatgtgcca gcgccagttc cacgcgactc cggcacatgt gtatcagaat tatcaggcgc
     1321 aggtgcggct gatggtgaag ctgcgcttcc ttgccgggct ggcgcatcgg atcgcagaca
     1381 ccaacggcac gctcggcttt ccgcaggttc gtgaggtgct tgggcagatt gccgcgaatg
     1441 tcggattggt ggaggggctg gtctggggca tggaagcgcg tggcggcatg gcgggccgct
     1501 attacgtgcc tgatcgcaat ctgctgtaca cggcgcaggt ggtcacgcag cagatgcatc
     1561 cgcagatgat gacgctgatc cgggaactgg ccggcggcgg gatgatcatg ctgccgtcgt
     1621 ccgtggcaga ttttgcggac gccgagcttg ccagcctgat ccggcgaagc caatcgtcgc
     1681 cgttctacgg tgccgcggat cgggtgaagt tcttcaaact cgcgtgggac gcggtcgggt
     1741 cggaatttgg ctcgcgccac acgcagtatg agatgttcta tgcgggggct actttcgtca
     1801 ccaaggcaca cgcgttccgc acctatgact ggaaacggtc gacggcgatg gtcgacgggg
     1861 tgatggatgg gatcgatccg gtctgagtca gcggtttacg gcgccgtcag gaatcagggg
     1921 ggtggaagtt cccggttgcg cgaacccggg gtttgcggca aggacaggtc ggccgcgcgg
     1981 cggtctgttg tcgatgcgaa atctccaatg cctggcatga gaatctccga tttaatactt
     2041 gcaatcacga agccgtgagt ggtaaacttc tgttcatccg agcgcgttgg cggccgcccg
     2101 cttgcctggc gttgatcgga tggggggcca tctcgggtta tgctcgggta cccccgactg
     2161 cgcgaccggg gggattcgat gagcggacgg tgttttgatt atcctctgcc caagggcttc
     2221 gctttcgaca ttcccgaatt ggagatgctt cagcgctggg ctgagggcca ggccatgcgc
     2281 atggtggtcg agctggatca ctgcgtcgaa ggtgaagaat acgaagaggt tctggccttc
     2341 tacgaagatg gcagtgcact gcgccgctgg accatgtggc gcgccgccgg ctatttcgtg
     2401 ctcgaaccga tgaacggccc ggactggcgg tttgccgaac tgccggacct gatcgccgag
     2461 atggtcgcgc tgcgttcatg atggggcggg gccaacgcgc ctcggcggac gtaaacgggg
     2521 atcggcgctg agttcgagca gatcgatctc ctcctccacg gcgtgaaagg cgtcgtcgcc
     2581 gatccgaccg ctgcgccgca aatcgcccag cacgttccgc tgtcttgcca ccacccgccg
     2641 cagcgcggct gaaggatccc ggggcgaggc atcgaacgcc gcatccagta gcgtcggcgc
     2701 gacgaagagc gcaagcgcga gggtggggtc cagggccgct cggggtgcgc ctgggacgaa
     2761 ggcgaccgcg gtgcgggcca gtgccagcaa cgaggggtag ggtacctgaa gccaacgcgc
     2821 gagacgcgcg agcaacgccg cgatcacaag cagggcgatc gtgaggtcga agacggccat
     2881 gtgctagacg tgattgcttc aagttgaagc aatcacgtct agcttgatct gtttgagagc
     2941 gtgattcacg ctttctggcg ttccgcctca agtgatcacg ctctcgttcc ccgcattcca
     3001 gcaaaccgga gcttgcgggc gaagcgcggg gcgggaaagt ggggggatca tgcggttggc
     3061 gggcatgtcg ttcgagcgta gggttttgcg aacgatcgga ggcgggtcat ggatttcggc
     3121 gcggcgatgt tcttcaccga ctactcgatg gcgccggatg agcttgcccg cgccctggaa
     3181 gagcggcgtt tcgagtcggt ctgggcgccc gaacactccc atatcccgct gtcgcggcag
     3241 tcaccgttcc ctggcggcgg cgatctaccc aagcggtact acgacacgat ggacccgttc
     3301 gtcactctca ccgccgcggc cgccgcgacg aagcggttga ggatcggcac cggtgtggcg
     3361 ctggtcgtgc agcgggatcc gatccagacc gccaagctgg ttgcctcgat cgatcagatt
     3421 tccggcgggc ggtttctgtt cggcgtgggg aacggctgga acgccgagga gatggccgat
     3481 cacggcacca aggacttcaa gtcgcgacac aagctggtgc gtgagcgcat cgaggcgatg
     3541 aaggcgatct ggac
//
LOCUS       DATIMH010000730         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1911793, whole genome shotgun sequence.
ACCESSION   DATIMH010000730 DATIMH010000000
VERSION     DATIMH010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911793"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1589
                     /locus_tag="VLI90_12840"
     CDS             147..1589
                     /locus_tag="VLI90_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSV15138.1"
                     /translation="MSSDQTLPDKQKKRKGPKHWAMFVLRWGIAVAGVWYVLAHMTLR
                     DQAWVILDKTTNRPQQVLLAHRVPEDAATFPIIDPKTHHVVDLPRADVVNEPDQKSVQ
                     LRDGTFRKLLALDLIGDLNHNARVDQFLVEDPHTLNGVWIPPSEVQNYEVKVPHPRDQ
                     VGLMSMIVDARPALLWASVCVFVLTFIITSYRWHELLKALDIHLTLGRTFAINMVACF
                     YNTFMPGSTGGDLVKAYYASKQTPHRMRAVMSVLVDRAIGLLALIFVGGVAAGRQWDI
                     PACRKVAIAAIAICGCVLVGLLIFYDPRLHRLSGLDFILRKLPKQHHVRAAIEAMHMY
                     GRRPLLVLWSLIISMPVHGTVILSAMFAGMAFGLPLHWSYYWVCVPVIVLAGAIPISP
                     QGAGVMEAFAVLLTRNQGVSVSQAFALAMSIRLVQILWNLTGVIFVIRGGYHAPSEKE
                     AAELEEDGDDDNAPTDSSPASGLGAPAVIT"
     gene            1597..>2236
                     /locus_tag="VLI90_12845"
     CDS             1597..>2236
                     /locus_tag="VLI90_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSV15139.1"
                     /translation="MLRKIDRILLRVPSLPAAVRYYTDSLGMKLVKQDQRLANLRFAD
                     NDTELVLHIDPDLPAEATYYLVDDVRDLYKRRAELRLAFNAPPTQASRGWRATVKDPF
                     GIVLLIIDRTGAGGENTSVVEDAKPAGALFAGVEQRVSVNREALIIAYEKIGRTADDL
                     PYTPHFESMWKSYIAAMNEPKPTRAETWRHLLNLRKAGKLPKMGEAKSKPPEV"
BASE COUNT          393 a          718 c          717 g          408 t
ORIGIN      
        1 tcccgcttac ttcgtgagta cactccgtgc gcggctctta tccggtttgg ccaagtactc
       61 gccgaatctg tcacgcaccc aagtgaccag gcctgatcgt gacagggttg cgtcgcgcgc
      121 ggtgtgcgga cacaatctgc cgcttcatga gttctgacca gacgttgccg gataaacaaa
      181 agaagcgcaa gggccccaag cactgggcga tgtttgtgct gcgctggggc atcgccgtcg
      241 ccggcgtgtg gtatgtgctg gcccacatga cgctgcgcga ccaggcgtgg gtcatcctgg
      301 acaagaccac caatcgcccg cagcaggtgt tgctggccca tcgcgtgccg gaagacgcgg
      361 cgacgtttcc gatcatcgat cccaagaccc accacgttgt cgatctgccg cgcgcggatg
      421 tcgtgaacga gccggatcaa aagtccgtgc agctgcgcga cggcacgttt agaaagttgc
      481 tggcgctgga cctgatcggc gacctgaacc ataacgcccg cgtggaccag ttcctggtcg
      541 aggatccgca cacgctgaac ggcgtgtgga tcccgccgag cgaggtgcag aactacgaag
      601 tgaaggtccc gcacccgcgc gatcaggtcg gtttgatgag catgatcgtt gatgcgcgcc
      661 cagcgctgct gtgggccagc gtgtgcgtct tcgtgctcac gttcatcatc accagttatc
      721 gctggcacga gctgctcaag gcgctggaca tccacctcac gcttggccgg acgttcgcga
      781 tcaacatggt cgcctgcttc tacaacacgt tcatgcccgg ctcgaccggt ggtgatctgg
      841 tcaaggcgta ctacgcatcg aagcagacac cgcatcgcat gcgcgcggtg atgagcgtgc
      901 tggtggaccg cgcgatcggc ctgctcgcgc tgatcttcgt cggcggcgtc gccgcggggc
      961 ggcagtggga tatccccgcg tgccgcaagg tggcaatcgc ggccatcgcc atctgcggct
     1021 gcgtgctggt tggtttgctc atcttctacg atccgcggct gcaccgcctc agcgggctcg
     1081 atttcatcct gcgaaagctg cccaagcagc atcacgtgcg cgcggccatc gaggcaatgc
     1141 atatgtacgg ccgccggccg ctgctggtcc tgtggtcgct gatcatctcg atgcccgtgc
     1201 acgggaccgt catcctcagc gcgatgttcg ccggcatggc cttcggcctg ccgctgcact
     1261 ggtcatacta ctgggtctgc gttccggtga tcgtgctcgc cggcgcgatt ccgatttccc
     1321 cgcaaggcgc cggcgtgatg gaagcgttcg cggtgctgct gacgcgcaac cagggcgtga
     1381 gcgtctcgca agcgttcgcg ctggcgatgt ccatccgcct cgtgcagatc ctgtggaacc
     1441 tcaccggcgt catcttcgtc atccgcggcg ggtatcacgc gccgtcggaa aaagaggcgg
     1501 ccgaactgga agaggacggc gatgacgaca acgcgccaac ggattcgtcg cccgcctccg
     1561 gtctgggcgc tccagcggtc atcacgtaac gcctccgtgc tccgcaaaat tgaccgcatc
     1621 ctcttaagag tccccagcct ccccgcggcg gtccgctact acaccgactc gctgggaatg
     1681 aagctggtca aacaggacca gcgcctggcg aacctgcggt tcgccgataa tgacaccgaa
     1741 ctggttttgc acattgaccc cgacctgccc gcggaggcga cgtactacct cgtcgatgac
     1801 gtgcgcgacc tgtacaagcg ccgcgcggaa ctgcggctgg cgttcaacgc tccaccgacg
     1861 caagcgtcgc gcgggtggcg cgccaccgtg aaagacccct ttggcatcgt cctgctgatc
     1921 atcgaccgca ccggcgcggg cggtgaaaat acgtccgtcg tcgaagacgc caagccggcg
     1981 ggggcgctgt tcgcgggcgt cgagcagcgc gtgtcggtca atcgcgaagc gctgatcatc
     2041 gcgtacgaga agatcgggcg gacggcggat gatctgccgt acacgccgca ctttgagtcg
     2101 atgtggaagt catacatcgc cgcgatgaac gagccgaaac cgacgcgcgc cgagacgtgg
     2161 cggcacctgc tgaacctgcg gaaggccggg aagctgccga agatgggcga ggcgaagtcg
     2221 aagccgccgg aggtgt
//
LOCUS       DATIMH010000731         1788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3641198, whole genome shotgun sequence.
ACCESSION   DATIMH010000731 DATIMH010000000
VERSION     DATIMH010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3641198"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VLI90_12850"
     CDS             <1..222
                     /locus_tag="VLI90_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15140.1"
                     /translation="TEAYFPNVPTPIDLPRQDLRAVCPAGIVRITIDCIVRNTGGIAS
                     FFGFEGFYLRLLVSVPQQIIWDARGEEAT"
     gene            complement(238..765)
                     /locus_tag="VLI90_12855"
     CDS             complement(238..765)
                     /locus_tag="VLI90_12855"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HSV15141.1"
                     /translation="MQKLAYCIFDTALGACGIGWTSERSVTHFQLPEATIERTASRVA
                     RLTAATSPSAAPPEVADVIDRVRAHLSGEPQDFDLVPVELNGVSDFERQVYRVTREIA
                     AGRTMTYGEIARAVNHSGAARVVGQALGRNPIPLIIPCHRVLAAGGKSGGFSAPGGRA
                     TKAKLLALEGVLLPF"
     gene            846..>1788
                     /locus_tag="VLI90_12860"
     CDS             846..>1788
                     /locus_tag="VLI90_12860"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HSV15142.1"
                     /translation="MKAAWLLGAMLTAALVTAVDLHAADEPAGSDSGPPQVPLPPRMT
                     RIFDGKTLDGWIQEPINAITFGSGDITDYKGLAKKLTNKSDPVSAFVADQLDDAAKAA
                     LAADPSADSGKALKSALSKNLTRFAAGPSIYDQTRFKGVQLRPETGELLQKRPAEGHE
                     LARLNHALLEDAYPSELAPTPAVSWTVKNGALASVGAGRGVIYTAKSFGRYRVVFDIR
                     HVSGNPDHQACVLVFCTAPTLGVKPLDALGGIQFQVPNGGHWDYRPGMNNNGGAEFMS
                     MTKTRFDPHEWCRVEILADAAKGVARMAVAQPPGSKAV"
BASE COUNT          340 a          586 c          562 g          300 t
ORIGIN      
        1 actgaagcct acttcccaaa tgttcccacc ccgattgatc tgccgcgaca ggatcttcgc
       61 gccgtttgcc ccgccgggat cgtccgcatc acgatcgact gcatcgttcg aaacactggc
      121 ggcatcgctt cgttctttgg ctttgaaggc ttctatctcc gcctgctggt gtccgtcccg
      181 cagcaaataa tttgggacgc ccgtggcgaa gaggcgacat agggcaggag ccgaaactca
      241 aaacggcagc aacacgcctt ccagcgccag cagttttgcc ttggtcgctc gaccgccggg
      301 cgcggagaac ccgccgctct tgccgccggc cgccagcacg cggtgacagg ggataatcag
      361 cgggatcgga ttgcggccca gcgcctgccc aacaactcgg gcggcgccgg aatggtttac
      421 cgcgcgtgcg atttcgccgt acgtcatcgt ccgcccggca gcgatctcgc gggtcacgcg
      481 atagacctgc cgctcgaaat cactgacgcc gttcaattcc actgggacga ggtcaaaatc
      541 ctgcggctca ccgctcaggt gcgcgcgaac gcgatcgatc acatcggcaa cctccggcgg
      601 cgcggcgctg ggtgatgtcg ccgcggtcag ccgcgcgacg cgcgatgcgg ttcgctcgat
      661 cgtcgcttcg ggcagttgaa aatgggtgac ggaacgctcg ctggtccacc cgatgccgca
      721 ggcgccgagg gcagtatcga agatgcagta cgcgagcttc tgcatggttg aaagtgagcg
      781 atgaaacgga cgccgccgtc gagagtatac gcatttgaac ggtcacactt caaagggagc
      841 gagcgatgaa agctgcatgg ctgcttggcg cgatgctgac cgccgcgttg gtgacggccg
      901 tcgatctgca cgcggccgac gaaccggcgg gaagcgacag tggcccgccg caggtcccgc
      961 tgccgccgag gatgacgcgg atcttcgacg gcaagacgct cgacggttgg attcaggaac
     1021 cgatcaacgc catcaccttc ggcagtggcg acatcaccga ttacaagggg ctggcgaaga
     1081 agctgaccaa caagtccgat ccggtatcgg ctttcgtggc ggatcagttg gacgacgccg
     1141 ctaaggccgc gctggcggcc gatccatctg ccgacagcgg aaaggcactg aagtccgcgc
     1201 tgtcgaagaa cctcacgcgg ttcgcggccg gtccttcgat ctacgaccag acgcggttca
     1261 agggggtgca gcttcggccg gagacgggcg aactactgca gaagcgaccg gccgaaggtc
     1321 acgagcttgc ccggctgaat cacgcgctgc tcgaggacgc gtatccatca gagctcgcgc
     1381 caacgcccgc ggtgtcgtgg acggtgaaga acggcgcgct cgccagcgtc ggcgccggtc
     1441 gcggcgtgat ctacaccgct aaatccttcg gccgatatcg cgttgtcttc gacatccgcc
     1501 acgtctcggg caaccccgac caccaggcct gcgtgctcgt cttctgcacc gcgccgacgc
     1561 tcggagtgaa accgctcgac gccctcggcg gcattcagtt ccaggttccc aacggaggcc
     1621 actgggacta ccgccccggc atgaacaaca acggaggcgc ggaattcatg tcgatgacca
     1681 agactcgctt tgaccctcac gaatggtgtc gcgtcgaaat cctcgcggac gctgccaaag
     1741 gcgtcgcgcg catggccgtc gcccaacctc ccggcagcaa agccgtcg
//
LOCUS       DATIMH010000732         5158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5088401, whole genome shotgun sequence.
ACCESSION   DATIMH010000732 DATIMH010000000
VERSION     DATIMH010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5088401"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VLI90_12865"
     CDS             complement(<1..298)
                     /locus_tag="VLI90_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15143.1"
                     /translation="MLATLIVFGQSIAFEFVSDDWIVILNNPHINPPTWGSFGYYWTH
                     IAWNLYMPVTCMVWAGLARFAQLSQVDASGFSLNPYVFHFASVTLHVATALAVYR"
     gene            complement(386..1738)
                     /locus_tag="VLI90_12870"
     CDS             complement(386..1738)
                     /locus_tag="VLI90_12870"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HSV15144.1"
                     /translation="MTSPAYHVASEHVAHAGAAKGTGKPGVNVSTPQTINRSIVPAAR
                     HWFWFVFALGLSMRLYLALFTQGTYDVSIWHQHAVGVHQQGLIAYYHSNHEMNHPPFI
                     AEVVAGLWDVSNASGLPFRILLRLPVIVVDVAIVALLLWLLRAEPRRFIVATIYWLNP
                     LAIIFSAYHGNIDTIVAFCILLCVCLLAKQRIIWAAIALGVSVWFKLPGVLVAPALAL
                     FVPSWPKRLRFSAVAAAVALSTYVPALLLDAPIVRDNVFAYHGQMIQTSGRIPIWGAR
                     LFLADFDRLSPATQEALAPVLTIYYEQNRAVCLGLILVWSWLRRRERSVRALSLTITG
                     VYCILYAFSNNWSFQYVAWSVPFWCLTNVWFALVATILAGGYIYGVYALACGNLWLLG
                     KWDFAGHPFWPQWLIDLRDAALLLFIATSVYLLAAAVYAEVRRLIPRRIQYQSASRSR
                     "
     gene            complement(1735..2214)
                     /locus_tag="VLI90_12875"
     CDS             complement(1735..2214)
                     /locus_tag="VLI90_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSV15145.1"
                     /translation="MDILLSITVFLAFGAAFVFLNMAVGALARPKAPNPEKSAVYECG
                     EPTIGSSWVQFDLRFYIVALVYLIFDVEVALFYPWAVAYGDAAAIGKPLGLTLFQVRQ
                     TALIDMLFFFGILLVGFAYLWRFGYLDWVRSAATTSLKIDYRPGVDLRSEIRADRPA"
     gene            2435..2866
                     /locus_tag="VLI90_12880"
     CDS             2435..2866
                     /locus_tag="VLI90_12880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HSV15146.1"
                     /translation="MQVRPYQSGDEEAVIALWQRCELVRPQNDPRKDIARKQRVQPEL
                     FLVAMDEQQQIIGSVMCGYEGHRGWINYLAVDLAHRRCGLGRALMAEAERVLRAAGCP
                     KINLQVRSSNAAVIAFYVAIGFAQDDVVSMGKRLERDETVQ"
     gene            3231..3518
                     /locus_tag="VLI90_12885"
     CDS             3231..3518
                     /locus_tag="VLI90_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HSV15147.1"
                     /translation="MKTIDIAKTSGQLREYAKNAKREVVVVTQRGKPLAAVVGLDDFD
                     YESLSLSTNAAFIDIIARSRARLEKEGGIPAAEVRRKLGLAPARMKRKPKS"
     gene            complement(3515..3853)
                     /locus_tag="VLI90_12890"
     CDS             complement(3515..3853)
                     /locus_tag="VLI90_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV15148.1"
                     /translation="MADRVVSSSLSADVIELLTKRGMTLAAIAEAIGVTRSFLSRVKG
                     RSRSLTIDHLVALEAVIGEPLPLLLLRATPIEAVPKDLRPLYRSTTRVVRGYPCKTKP
                     RTGRSSKVIS"
     gene            complement(3864..4142)
                     /locus_tag="VLI90_12895"
     CDS             complement(3864..4142)
                     /locus_tag="VLI90_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15149.1"
                     /translation="MAYRRPFTLQQRREAMAALRSYEKHRQLLRRSYPPSDREESATY
                     DQYVQAIEEMRQELLRADRTVRVERLNRLDAEARWLATEYWRLRIGKQ"
     gene            complement(4193..4891)
                     /locus_tag="VLI90_12900"
     CDS             complement(4193..4891)
                     /locus_tag="VLI90_12900"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSV15150.1"
                     /translation="MSHDSGSISLPSGFNAGGGFSVAPALNANVLVLNRYYQAIRVIN
                     VRRAFSLLCRDLAEVIHIEKDASGQSKWQNLDFNEWQELSQLKAEFEPAEHDWIHTVR
                     FAIAVPRIIRLLGYDKLPRQDVKFNRRNIYARDSNRCQYCGKRMPTTELSLDHVVPKS
                     QGGKSSWTNIVCCCVKCNVKKGGRTPEQAHMHLITKPVKPKRSPVINIKLADERYSSW
                     KQFLDTAYWTVELK"
BASE COUNT         1116 a         1517 c         1601 g          924 t
ORIGIN      
        1 gccgatagac cgccagcgcc gtcgcgacgt gcagcgtcac gctggcgaaa tggaagacat
       61 atggattcag cgaaaagcca ctcgcatcga cctgggaaag ctgcgcgaac cgcgccagcc
      121 ccgcccacac catgcacgtc accggcatgt acaggttcca cgcgatgtgc gtccagtaat
      181 agccgaagga tccccacgtc ggcggattga tgtgcgggtt gttcaggatg acgatccagt
      241 cgtccgaaac gaattcgaat gcgatcgatt ggccgaagac gatcagcgtc gccagcacga
      301 cagtgaccgg cggcgcccac cgttgccatg cacgggtccg tcgggccaga ggatgtgtgg
      361 cgtcggatga catgggcggg gtcgatcatc gcgagcgtga agcgctctgg tattggattc
      421 gccggggaat caatcggcgc acttccgcat acaccgccgc ggccaacagg tatacggaag
      481 tggcgatgaa caagagcagc gctgcatcgc gaaggtcaat gagccattgc ggccaaaaag
      541 gatgaccggc aaagtcccat ttaccgagca gccacaggtt cccgcacgcg agcgcataca
      601 ctccatagat gtaacccccg gccaagatgg tggcgaccag cgcgaaccac acattggtca
      661 ggcaccagaa cggcaccgac catgcaacat attgaaacga ccagttgttt gagaaggcat
      721 acagaatgca atagacgccg gtgatcgtga gcgacagggc gcggacgctt cgctcgcggc
      781 ggcggagcca ggaccatact aagatcaaac caagacagac ggctcggttc tgttcatagt
      841 aaatcgtcag gaccggcgcc agtgcttcct gcgttgcggg ggacagtcga tcgaaatcgg
      901 ccaagaacaa tcgcgcgccc cagatgggaa ttcggccgct cgtctgaatc atttggccgt
      961 ggtatgcgaa gacgttatcg cggacgatcg gcgcgtcgag cagcaatgcc ggcacgtagg
     1021 ttgagagcgc gacggcagcc gcgaccgctg aaaacctcag ccgtttcggc cacgagggca
     1081 cgaacagcgc cagtgccggc gccaccagga cccccggcag tttgaaccac acgctcacgc
     1141 ccagcgcgat cgccgcccag atgatccgct gcttggccag caggcagacg cagagcagga
     1201 tgcagaaagc gacgatcgtg tcgatgttgc cgtggtacgc ggagaagatg atcgccagtg
     1261 ggttcaacca ataaatggtg gcaactataa atcggcgcgg ctcggcgcgc agcagccaca
     1321 gtagaagcgc cacgattgct acatccacca cgatgaccgg caatcgcagt aatatcctaa
     1381 atggtaagcc ggacgcattc gaaacatccc acagccccgc gaccacctcg gcgatgaacg
     1441 gcggatggtt catctcatga ttgctatggt agtaggcgat taatccctgc tgatgcacgc
     1501 caaccgcgtg ctgatgccag atgctgacgt catatgtccc ctgggtgaac agcgccaggt
     1561 acaggcgcat cgacaatccg agcgcgaaga cgaaccagaa ccagtgccgc gcggcgggca
     1621 cgatcgatct gttgattgtt tgcggagtgg aaacattgac gccgggcttg cccgtgcctt
     1681 tggcggctcc ggcatgagcg acatgttcgc tcgcaacgtg ataggcgggc gacgtcatgc
     1741 gggtcgatcg gcgcggatct cgctgcgcag atccacgccg ggacgatagt cgatcttcag
     1801 gcttgtcgtc gccgccgatc gcacccagtc tagatagccg aaccgccaga ggtatgcgaa
     1861 gcccaccagc aggatgccga agaagaagag catgtcgatc agcgcggtct ggcgcacttg
     1921 aaacagggtg aggccgagcg gcttgccgat cgccgcggcg tcaccgtagg cgacggccca
     1981 gggataaaac agcgcgacct cgacgtcgaa gatcaggtac accagcgcaa cgatgtagaa
     2041 ccgcaggtcg aactgcaccc agctggagcc gatggtcggc tcgccgcatt cgtacaccgc
     2101 ggacttttcc ggattcggcg ccttgggccg ggccagcgcg cccacggcca tgttaagaaa
     2161 cacgaacgcc gccccgaagg cgaggaagac ggtgatggaa agcaggatgt ccatggcgaa
     2221 cgaatagatt acggcgcggc aggcggaaga tcaaaagtta atggcgcgcc cgctcgcgcg
     2281 cgtgaaatca gccccccgac gccggtgtct gaggtactcc gaagacccgg gttcttatgc
     2341 gcgaaagtac ccgcgtcttc ggagtacctc agacatcggc gtcgggtcgc gccattcgta
     2401 agaccctacc acttccggta gcgtctcttc agccatgcag gttcgacctt accagagcgg
     2461 tgacgaggaa gcggtgatcg cgctatggca acggtgcgag ctggtgcggc cgcagaacga
     2521 cccgcgcaag gacatcgccc gcaagcagcg cgtgcagccg gaactattcc tcgtggcgat
     2581 ggacgagcaa cagcaaatca tcggcagcgt gatgtgcggg tacgaaggcc accgcgggtg
     2641 gatcaactat ctggcggtcg atctcgcgca ccgccggtgt ggcttgggcc gcgcgctgat
     2701 ggccgaagcc gaacgcgtgc tgcgcgcggc ggggtgtccg aagatcaacc tgcaggtgcg
     2761 atccagcaac gcggcggtca tcgcgtttta tgttgcgatt ggatttgcgc aggacgacgt
     2821 ggtcagcatg ggcaaacggc tggagcggga tgagacagtc caatgagcga cgacggccca
     2881 gagaaagtcc cgcatcgtgt tgccaaatct ggaaaacacc gattaggttt cggtcatgca
     2941 ctttgaaatc gagtattcac agatgctttg aaccatttac gagagttgag caagcgcgac
     3001 cagcatacgg tggtggatca ggttgatcgg cacctcgttc accaaccgag cacaccgact
     3061 cggcaccgaa aaaagctgcg gctgaacgtc gtgacggact gggaactgcg cgtcggagat
     3121 ctgcgtgtat attatgtctt gcgagatccg ccgaatccgg tcgtctccat tgtggcagtg
     3181 ggcagaaaag tcggaagtcg agtattcatc gggggagttg aggtaacact gtgaagacca
     3241 tcgatatcgc aaagacatcg ggccagctgc gggaatacgc caagaacgcg aagcgggaag
     3301 tggtcgtcgt aacgcagcgc ggcaaaccgc ttgcggcggt ggttggtctg gatgatttcg
     3361 attacgagtc attaagcttg agcaccaacg ccgcgttcat cgacatcatc gccaggtcac
     3421 gtgcccgact cgaaaaagaa ggcgggattc cggctgcgga agtgcgccgc aagttggggt
     3481 tggcaccggc tcggatgaag cggaagccaa aatcctaaga gatgactttt gatgatcgcc
     3541 cagttcgagg ctttgttttg caaggataac cgcgcaccac gcgcgtcgtg gaacgataga
     3601 gcggccgaag atccttcggc accgcttcaa tgggcgtcgc gcgcagcagg agcagcggaa
     3661 gcggctcgcc gatgacggcc tccagcgcaa ccagatgatc gatcgtaaga ctccgggaac
     3721 gccctttcac acggctgagg aacgatcgtg tgacaccaat cgcctcggcg attgcggcga
     3781 gtgtcatgcc ccgcttcgtc agcagttcaa tcacatccgc cgatagcgaa gatgacacga
     3841 ctcgatccgc catgatgcac acctcactgc tttccgattc gcagccgcca atactccgtt
     3901 gccagccacc gggcttcggc atcgaggcga ttgagtcgct cgacccgcac cgtgcgatcc
     3961 gccctgagca gctcctgccg catttcttca atcgcctgga cgtactgatc gtacgtagcg
     4021 gattcttcgc ggtcagacgg agggtagctg cgccgcaaca gctgccgatg cttctcatac
     4081 gatctaagcg ccgccatcgc ctcacgccgc tgttgcagcg tgaacggacg ccggtaagcc
     4141 atcgcctgcc tttgttgaat ttagatgggt gttgcccaac ggtcaacaga aattatttga
     4201 gttcaaccgt ccaatacgcc gtgtccagga actgtttcca gctcgaatac cgctcgtcgg
     4261 cgagcttgat gttgatcacc gggctgcgct tgggtttgac cggcttggtg atcaggtgca
     4321 tgtgcgcctg ctcgggcgtt cggccgccct tcttcacgtt gcacttcacg cagcagcaga
     4381 cgatgttcgt ccagctgctc ttgccgccct ggctcttggg caccacgtga tccagcgaca
     4441 gctcggtggt gggcatccgc ttgccgcaat actggcagcg gttgctgtcg cgggcgtaga
     4501 tgttgcggcg gttgaacttc acgtcctgcc gggggagctt gtcgtaaccc agcaggcgga
     4561 tgatccgcgg caccgcgatc gcgaagcgaa cggtgtggat ccagtcatgt tccgccggtt
     4621 cgaactcggc cttcagctgg ctcagctcct gccattcgtt gaagtccagg ttctgccact
     4681 tgctttgacc gctggcgtcc ttctcgatgt ggatgacttc ggccaggtca cggcacagca
     4741 gcgaaaacgc acgccgcacg ttgatgacgc ggatggcctg gtaatatcgg ttgagaacga
     4801 gaacgttggc gttgagcgcc ggcgcgaccg aaaagccgcc accggcgtta aagcccgagg
     4861 ggagcgagat tgaccccgag tcgtggctca tacctgtaac agaccaggca gccttctgca
     4921 tttgctccgc gatgccccgc cactcgagcg gtcgcccgcg atttgagatg cccgacggaa
     4981 gtcatgacgt tcgtcatctg accctccatc gcccggaagg cacggctaat tgtaccaacc
     5041 tcgacgtgca aaggccagtg ggatcgagca cttgccagat agcggtctcg cagtcgccgg
     5101 cgacgccggt gtctgaggta ctccgaagac ccggatcggt tgcgcgaagg atccgggt
//
LOCUS       DATIMH010000733         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1912286, whole genome shotgun sequence.
ACCESSION   DATIMH010000733 DATIMH010000000
VERSION     DATIMH010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912286"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /gene="pruA"
                     /locus_tag="VLI90_12905"
     CDS             complement(<1..1463)
                     /gene="pruA"
                     /locus_tag="VLI90_12905"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HSV15151.1"
                     /translation="MSTPTSTIPTPTAATTKSDMAATSTFAAFGGIRRVPQPINEPNK
                     SYAPGSPERAELKARLKAMASERIDIPLVIAGQEVRTGRIAQAVMPHDHKHVLADYHL
                     AGPDHVQQAVAAAAEARREWGSWPWEERAAVLLRAAELLATTWRATINAATMLCQSKT
                     VFQAEIDAASEMIDFWRFNAFFAQELYSEQPISGPGVWNQMEYRPLEGFVYAVSPFNF
                     TAIGGNLTTAPALMGNTVIWKPASSAMLSGYYTLKVLEAAGLPPGVINFLPGDPREIS
                     NQLIDSSELAGIHFTGSTEVFNGMWKKIGENVGRYRGYPRLVGETGGKDFIVVHPSAD
                     PSEVAVAIARGGFEYQGQKCSAASRVYIPRSLWNDVRERTVAMMKAIKIGDVADFRNF
                     MGAVIDQKAFTKISGYLDDAKRNAKVISGGTATGDQGYFIEPTLIETTDPGYRSLCEE
                     IFGPVVTAYVYPDNAWRETLDIVDRTSPYALTGAVFSR"
BASE COUNT          288 a          532 c          525 g          337 t
ORIGIN      
        1 cggctgaaca ccgcgccggt caacgcgtag ggcgacgtgc ggtccacgat gtcgagcgtc
       61 tcgcgccagg cattgtctgg atacacatac gccgtcacga caggaccgaa gatctcttcg
      121 cacagcgatc gatatccggg atccgttgtc tcgatgagag tcggctcgat gaaataaccc
      181 tgatcacccg ttgcggttcc acccgaaatg accttcgcat tccgcttcgc atcatccaga
      241 tacccgctga tcttggtgaa cgccttctga tcgatcaccg ctcccatgaa attgcggaag
      301 tcggccacgt cgccaatctt gatcgccttc atcatcgcca cggttcgctc acgaacgtcg
      361 ttccacagcg aacgcgggat gtagacccgg cttgccgctg agcacttctg tccctggtac
      421 tcgaatccgc cgcgcgcgat tgctaccgca acttcggatg gatcggccga ggggtgcacg
      481 acgatgaagt ctttgccgcc ggtttcgccg accagtcgcg gatagccgcg gtagcggccg
      541 acgttctcac cgatcttctt ccacatcccg ttgaacacct ccgtgctccc ggtgaaatgg
      601 atgccggcga gctcggagga gtcaatcaat tgattgctga tctcgcgcgg atctccaggc
      661 aggaagttga tcaccccggg cggcaggccg gctgcttcga gcaccttcag cgtgtagtag
      721 ccgctcagca tcgcgctgga tgcgggcttc cagatcacgg tattccccat caacgcgggc
      781 gcggtcgtca ggttgccgcc gatcgcggtg aagttgaagg gggacacggc gtagacgaat
      841 ccctcgagcg ggcgatactc catctgattc cacacgcccg ggccgctgat cggctgctcg
      901 ctgtagagct cctgcgcaaa gaaggcgttg aagcgccaga agtcgatcat ctccgaggcc
      961 gcatcgatct ccgcctggaa caccgtcttc gactggcaga gcatcgtggc cgcattgatc
     1021 gtcgctcgcc atgtcgtcgc cagcagttcc gccgcgcgca gcagcaccgc ggcacgctct
     1081 tcccatggcc agcttcccca ctcgcggcgc gcctcggcgg ccgcggcgac ggcctgctgc
     1141 acgtggtcag gtccagcgag gtgatagtcg gcgaggacgt gtttgtgatc gtgcggcatc
     1201 accgcttgcg cgatgcgccc cgtcctcact tcctggccgg cgatcaccag cgggatgtcg
     1261 atgcgctcgc tcgccatcgc tttcagccgc gccttcagct ccgcgcgttc cggcgagccg
     1321 ggtgcgtacg acttgttcgg ttcgttgatc ggttgcggca cacggcggat gccgccgaac
     1381 gccgcgaatg tcgacgtcgc cgccatgtcg ctcttcgtcg ttgcggctgt gggcgtagga
     1441 atggtcgaag tgggtgtgga catatcgttt ctcgtaatga agatgattgt actcgctacc
     1501 gggatccgtg gtccggggtc cgggatccgt ccgtcttggt ggctacgccg cttcggcggg
     1561 acagcttcgc cggtgaacgt gtcgcgaagc tgggccgagg cggaactgga aactgcgaac
     1621 tggggagcgt gggactgaaa actgggaact gggaactgga aactggaacc tggcagctgg
     1681 ca
//
LOCUS       DATIMH010000734         5985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_464547, whole genome shotgun sequence.
ACCESSION   DATIMH010000734 DATIMH010000000
VERSION     DATIMH010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464547"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2955)
                     /locus_tag="VLI90_12910"
     CDS             complement(<1..2955)
                     /locus_tag="VLI90_12910"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HSV15152.1"
                     /translation="MRIVIAVMLAGLLAASRAFGQFTPSSPIYELRSSSLDQSEDVTI
                     EAWLKIDSACPAGARVIDKWGPGSDVGYRLEVAKDGSLQWLTTAPAECGTGKPLPNDR
                     AAHVIAVFSPRATRAEIYVDGKLAASVPAANRRFDLPLTSTPLRIGADQDGGNRFIGS
                     IDRVAIYKRALSSDEIAAREKPADVAAGLIGDWQFTAGGVHASADAPELRVAPQITPA
                     SALPEGKLTMWYRRPAREWVEALPVGNGRLGAMVFGGVDQERLQLNEDTIWSGGPYGP
                     VNPDAADALPKARELIFAGKQKEAEELIKAKIMSKPVGQASYQTLGNLLLRSAAGDAM
                     RVSDYRRSLDIDTAIATTTFKIGQTTFTREVWCSAPDQVLVVRLTADQPGSISFSATM
                     QTPQPDAALLMRGNDLILTGTGGKHGTIPGAVKFTGIVRAQNEGGSVTAAGSELTVTG
                     ANAVTLLVSAATNYVNWHDVSGDANARAMSRLDAASGKSVDQLRLRHVQDYQALFHRV
                     SIDLGTASRTSAGQPTDERVKHFAGGDDPQLAALFYQFGRYLLISSSRPGGQAANLQG
                     LWNDLTSPPWGGKYTVNINTEMNYWPAETANLSECAEPLFQLVREIAETGARTASEMY
                     HARGWVCHHNTDAWRATGPIDFPATGMWPVGGAWLTTHLWEHYQFTGDRAALAAAYPI
                     FKGACEFFLDTLVEEPTHHWLVTCPSVSPEHGGVVAGPTMDLQILRDLFAQTAKASQV
                     LGIDEDFCKQVLATRDRLAPMQIGKFGQLQEWLEDKDRERDSHRHQSHLYGLFPSSQI
                     NPETPELFAAARKSLLGRGDAATGWSLAWKMNLWARELDGDHAYLLLSRLLREPQTGP
                     DSGGERGGGTYPNLFDAHPPFQIDGNFGATSGITEMLLQSQEGILRLLPALPRAWPSG
                     SASGLCARGGFIVDEAWSDNRLTQATIQSRLGGPCMIRSAAPFTISEQATPVAVESAG
                     GGEYLFQTQAGHA"
     gene            complement(2981..>5985)
                     /locus_tag="VLI90_12915"
     CDS             complement(2981..>5985)
                     /locus_tag="VLI90_12915"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HSV15153.1"
                     /translation="GTRQYFKNCSISGSVDFIYGAATGIFDTCTITQRVGGVSLTAPA
                     TPVGNYGLVFINSHLVNGGAITAKTSALARAWNAPYGQSVFVHTNMDNMISDAGWTNF
                     STTPQYTATERYDEYDSSGAGAAGYLAVPTKRAAQAVNMTAAQAAVFADITTWTPNFT
                     TLFGGWNPNQAWAMIPATFTAASGAWETGGNWDVGQAPVAGQAAYAPSGSITVSAADS
                     CDSLTLGTTAGGATLSVNAGASLRLGGALNTNSNGSPISKVTVSGAFTVGTVILGNSG
                     DELHLTNNATFNLLSGWGGAWAAGKLYVDNATLAAMNLGGKFAGPDLYLAAASGQSGT
                     WNIQPGNTYYLHAIRTGAGTSTITMTGGTVYGAYGPSFGYAGGGGAVTFNMKGGEFDV
                     FNPNLGAGAGTYVFNQTGGTFQKDMNTFATSTGYDWLLGDASGANATWNLSQADGSAI
                     VNIRDLFVGQKGTGKFAQTAGAVTINQEDATQVASHSGMRLGWGAGGNGTYEISGGSL
                     SIANGGITNGYDPASGSWGTGTFRVLGGAAGTINVTGNYSQSSHSLLDDHVNASGLST
                     MSITGNVSFASGAAVNLSADAGAAPGTYTLMSWTGSATGTPVLAAGVDTSRWSIAMGA
                     HALTATLLPLSSVAGTSGNDAIRLVRNSSSLDIYINNPTTPAYSVPFASLGALSVNSL
                     GGNDSITVDFGGGASPVPSGGLSIDGGANSDVLIIAGTTGADTATVNATTVIFNNSPI
                     TYANVESISINGTSGNDVLTQTAQPGNSATLSFSGSGAKTLNLNGGSFALNSDASAAT
                     PNLTVNVNAGGSVTFATTQHLTALNITGGGIASMSAAASPASPAVLNVTTLGISGAGS
                     KLDLADNEMWTTATVANVWAMIQGGQIASSSTGGALGYAGQASGVVEVRYTLIGDANL
                     DGVVDVGDLGALASSYGMSGGATWAQGDFNGNGAVDVGDLGALATKYGSNVSSLPATA
                     AATFAMSSTNDAFAIDEPVRKLIDDITT"
BASE COUNT          960 a         2049 c         1884 g         1092 t
ORIGIN      
        1 cgcgtgtccc gcctgcgttt gaaagaggta ttcgccgccg ccggcggatt cgacggcaac
       61 cggcgtcgcc tgctccgaaa tggtaaatgg ggctgccgat ctgatcatgc acggaccgcc
      121 gaggcgcgac tgaatcgtcg cttgcgtcag gcgattatcg gaccacgctt catcgacgat
      181 aaatccgccg cgggcgcaca ggccgctcgc gctgccgctg ggccacgccc tcggcagcgc
      241 cggcagcagc cgcagaatgc cttcctgact ttgcagcagc atctcggtaa tcccggacgt
      301 cgcgccgaag ttcccgtcga tctggaaggg cgggtgcgca tcgaacaggt tcggataagt
      361 gccgccgcca cgctcgccgc cggaatcggg gccggtctgc ggctcgcgca gcaggcggct
      421 cagcagcagg tacgcatgat cgccgtccag ctcccgcgcc cacaggttca tcttccacgc
      481 cagcgaccaa cccgtcgccg catcgccccg gccgagcaat gacttccgcg ccgccgcgaa
      541 cagttccggc gtttccggat taatctggct gctcgggaac aagccgtaca gatgcgactg
      601 atggcggtgc gaatcgcgct cgcggtcctt gtcctccagc cattcctgca gctggccgaa
      661 cttgccgatc tgcatcggcg ccagccgatc gcgcgtcgcc aggacctgtt tgcaaaaatc
      721 ctcgtcaatg cccaacactt gtgacgcttt cgcggtctgc gcgaagaggt cacgcaggat
      781 ctgcaaatcc atcgtcggcc ccgcgacgac gccgccatgt tccggcgaga ccgaggggca
      841 cgtcacgagc cagtgatgcg tcggctcttc aacaagcgtg tcgaggaaga attcgcacgc
      901 ccccttgaag atcggatacg ccgccgccag cgcggcccgg tcgccggtga actggtagtg
      961 ttcccacagg tgcgtcgtca accaggcgcc gccgaccggc cacatgccgg tggcgggaaa
     1021 gtcgatcggg ccagtcgcgc gccacgcatc ggtgttgtga tgacaaaccc acccgcgggc
     1081 atggtacatc tcgctcgcgg ttcgcgcgcc ggtctcggca atctcgcgca ccagctggaa
     1141 caacggctcg gcgcactccg ataaattcgc cgtctccgcc ggccaataat tcatctccgt
     1201 gttgatgttg accgtgtact tcccgcccca cggcggcgag gtcaaatcat tccacagccc
     1261 ctgaagattc gccgcctgcc cacccggccg gctgctggag atcagcaggt atcgcccgaa
     1321 ctggtagaac aacgccgcca gttgcggatc atcgccgccg gcgaaatgct tcacccgctc
     1381 gtccgtcggt tgccccgcgg atgttctcga agctgtcccc agatcgatcg acacgcggtg
     1441 aaacagtgcc tggtaatcct gcacgtgccg caatcgcaac tgatcgacgc tcttcccgct
     1501 ggcggcgtcg aggcgactca tcgcccgcgc gttcgcgtcg ccgctcacat cgtgccagtt
     1561 gacgtaattc gtggcggcgg acacgagcaa cgtgaccgcg tttgcgccag tgacggtcag
     1621 ttccgatccc gcggcggtga cggacccgcc ttcattttgc gcgcgcacga tgccggtgaa
     1681 cttcaccgct cccggaatcg tcccgtgctt tccgccggtg ccggtcagga tcagatcgtt
     1741 tccgcgcatg agtaacgccg cgtccggctg cggcgtctgc atcgtcgcgc tgaacgagat
     1801 gctgccgggt tggtcggccg tcaggcgaac caccagcacc tgatccggcg cgctgcacca
     1861 gacttcgcgc gtgaacgtcg tctgcccgat cttgaaggtc gtcgtcgcga tggcggtgtc
     1921 gatgtccagc gatcgccggt agtcgctgac gcgcattgca tcgcccgcgg cggatcgcag
     1981 cagcagattg cccagcgtct ggtacgacgc ctggcccacc ggcttggaca tgatcttcgc
     2041 cttgatcagc tcttccgctt ccttctgctt tcccgcgaag atcaactcgc gcgccttcgg
     2101 cagcgcgtcg gcggcgtcgg gattgaccgg gccatatggc ccgccggacc agatcgtgtc
     2161 ttcattcagc tgcagccgtt cctgatcgac accgccgaag accatcgcgc cgagccggcc
     2221 gttgcccacg ggcaatgcct cgacccactc gcgcgccggc cggcgatacc acatcgtcag
     2281 cttgccttcg ggcagagcgc tcgccggcgt gatctgcggc gcgacgcgaa gctccggggc
     2341 atccgcggag gcgtgaacac cacccgcggt gaactgccag tcgcctatca atccagcagc
     2401 gacatccgcc ggcttctcgc gcgccgcgat ttcgtcggat gacagcgcac gcttgtaaat
     2461 cgcaacgcga tcgatcgacc cgatgaaccg gttgccgcca tcttgatccg cgccgatgcg
     2521 gagcggcgtg ctcgtcagcg gcaggtcgaa ccgccggttc gccgccggca cgctggcggc
     2581 gagcttgccg tccacataaa tctccgcccg cgtggcgcgc gggctgaaca cggcaattac
     2641 gtgcgcagct cgatcgtttg ggagtggctt accagtgccg cattctgccg gcgccgtcgt
     2701 cagccattgc agcgagccgt ccttcgcgac ttcgagccga tagccgacat cggagccggg
     2761 tccccatttg tcgatgacgc gcgcccccgc gggacatgcg gaatcgatct tcagccacgc
     2821 ctcgatcgtt acgtcttcgc tctggtcgag cgaggatgaa cgcagctcgt aaatggggga
     2881 ggacggtgtg aattggccaa acgcgcgcga cgccgcaagc aaccccgcca acatcacggc
     2941 gatcacgatc ctcattggat gattcatgcc cttgcgctgt tcaagttgtg atgtcatcga
     3001 tcagcttgcg caccggctcg tcgatcgcaa acgcatcgtt ggtcgaggac atcgcgaacg
     3061 tcgcggcggc ggtggcgggg aggctgctca cattcgagcc gtattttgtg gcaagcgcgc
     3121 ccaggtcgcc gacatcgacc gcgccgttgc cattgaaatc gccctgcgcc caggtggcgc
     3181 cgccgctcat gccgtagctg ctggccaagg cgcccaggtc gccgacgtcg accacgccat
     3241 ccaggttcgc atcgccaatg agcgtgtagc gcacttccac cacgccactg gcttggcccg
     3301 cgtagccgag cgcgccgccc gtcgacgaac tggcgatctg cccgccttgg atcatcgccc
     3361 acacgttggc gacggtcgcc gtcgtccaca tctcgttgtc cgccaggtcc aacttcgaac
     3421 cggcgccact gatgccgagc gtcgtcacgt tcagcaccgc cggtgatgcc ggcgaagccg
     3481 cggccgacat gctcgcgatg ccaccgccgg tgatgttcaa cgccgtcaga tgctgcgttg
     3541 ttgcgaaggt cacgctgccg ccggcgttca cgttcaccgt gaggttcggc gtcgcggcgc
     3601 tcgcgtcgct gttgagcgcg aacgaaccgc cgttgaggtt tagcgtcttc gctccgctgc
     3661 cgctgaacga cagggtggcg gaattgccgg gctgcgcggt ctgcgtcagc acgtcattgc
     3721 cgctcgtgcc gttgatgctg atcgactcga cgttcgcgta cgtaatcggc gagttgttga
     3781 atatgacggt cgtcgcattg accgtcgcgg tgtcggcccc cgtcgttccc gcgataatca
     3841 gcacatcgct gttcgcgccg ccatcgatgc tcagcccacc gctcggcacc ggcgaagcgc
     3901 cgccgccgaa atcgaccgtg atgctatcgt tcccgccgag gctgttgacc gacagcgcgc
     3961 ccagcgacgc gaacggcacc gagtacgccg gcgtcgttgg attgttgatg tagatgtcga
     4021 gcgatgaact gttgcgaacg aggcgaatcg cgtcgttgcc gctggtgccg gcaacactcg
     4081 acagcggcag cagcgtggcg gtgagcgcat gcgcccccat cgcgatgctc cagcgcgacg
     4141 tgtccacccc cgcggccagc accggcgtgc cggtcgcgct gccggtccaa ctcatgagcg
     4201 tgtacgtgcc cggcgcagcg ccggcgtcgg cggacagatt caccgcggcg ccgctggcga
     4261 agctgacgtt gccggtgatc gacatcgtcg agagcccgct ggcgttgacg tgatcatcga
     4321 gaagcgaatg gctgctctgg ctgtaattgc cggtgacgtt gatcgtccca gccgcgccgc
     4381 cgagcacgcg gaacgtgccg gtgccccagg aaccggaggc gggatcgtag ccgttggtga
     4441 tgccgccgtt ggcgatcgac agcgagccgc cgctgatctc ataggtgccg ttgccgccgg
     4501 cgccccaacc gaggcgcatg ccggagtggc tcgccacctg tgtcgcatct tcctgattga
     4561 tggtcacagc gccggccgtt tgcgcgaact tgccggttcc cttctggccg acgaacaaat
     4621 cgcggatgtt cacgatcgcg ctgccgtccg cctggctgag attccacgtt gcattcgcgc
     4681 cgctggcgtc gcccagcaac cagtcgtagc cggtgctggt ggcgaacgtg ttcatgtcct
     4741 tctggaatgt cccgccggtt tggttgaaga cgtaggtgcc ggctccggcg ccgaggttgg
     4801 ggttaaagac gtcgaactcg ccacccttca tgttgaaggt gacggcgcca ccgccgccgg
     4861 cgtaaccgaa ggacggcccg tacgcgccgt acacggtgcc gccggtcatg gtgatggtcg
     4921 acgtgccggc gccggtgcgg atcgcgtgca ggtaataggt gttaccgggc tggatgttcc
     4981 acgtcccgct ttgcccgctg gccgcggcga gataaaggtc cggccccgcg aatttgccgc
     5041 ccagattcat ggcggccagc gtcgcgttat cgacatacaa cttccctgcc gcccaagcgc
     5101 caccccagcc gctcagaagg ttgaacgtgg cgttgttcgt caggtgcagt tcatcaccac
     5161 tgttgccgag gatcacggtg ccgacggtga acgcacccga cacagtcacc ttgctgatgg
     5221 gcgagccgtt gctgttggtg ttcagcgccc cgcccaatct gagcgacgcg cccgcgttga
     5281 cgctcagcgt cgcgccaccg gcggtggtgc cgagcgtgag cgaatcacac gaatccgccg
     5341 ccgacaccgt gatcgacccg ctgggcgcat aagccgcttg accggccacg ggcgcttggc
     5401 cgacgtccca attgccgccg gtttcccaag caccgctggc cgcggtgaag gtcgcgggaa
     5461 tcatcgccca cgcctggttg gggttccacc cgccgaagag cgttgtgaag ttcggcgtcc
     5521 acgtcgtgat gtccgcaaac accgccgcct gcgcggccgt catgttcacc gcctgcgccg
     5581 cgcgcttggt aggcaccgcc aggtaacccg ccgcgccggc gccggagctg tcgtattcat
     5641 cgtatcgctc ggtcgcggtg tactgcggcg tggtcgagaa gttcgtccag ccggcgtcgc
     5701 tgatcatgtt gtccatgttc gtgtgaacga acaccgactg cccatacggc gcgttccacg
     5761 cccgggccag cgcgctcgtc ttcgccgtga tggctccgcc gttcaccagg tgcgaattga
     5821 tgaagaccag cccgtaattt cccaccggtg tcgccggcgc cgtcagcgag acgccgccca
     5881 cgcgctgcgt gatcgtgcag gtgtcgaaga tccccgtggc agcgccgtaa atgaagtcaa
     5941 cgctgccgct gattgaacag ttcttgaagt actgccgggt gccgc
//
LOCUS       DATIMH010000735         5609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5088882, whole genome shotgun sequence.
ACCESSION   DATIMH010000735 DATIMH010000000
VERSION     DATIMH010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5088882"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..623)
                     /locus_tag="VLI90_12920"
     CDS             complement(51..623)
                     /locus_tag="VLI90_12920"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HSV15154.1"
                     /translation="MAGQEGPGSTYTGQDVGHAIVAEARDTLARALAKINHCLDQLSD
                     ADVAARPAAGMNCIAIIANHLAGNLQQWICAGLGNEPDTRYRPGEFDDPTDASVAAVR
                     RTLSDAVTAADRVLARLDPADLLRVRKVQGFNVTGTHALFDTAAHFVGHTHQIVQLTR
                     LRRGSQYRFQFVPETVEQGAPPQVARATTP"
     gene            complement(816..1538)
                     /locus_tag="VLI90_12925"
     CDS             complement(816..1538)
                     /locus_tag="VLI90_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15155.1"
                     /translation="MAPVAQPALAARISEPQPIPIWASVLVILLCLGGGGWIMHWYVT
                     SDPLSHETKLLDPGVIPAAPTRTPPSARGNNMAAAQAAPAVRKQDDTSWWVHAPEAAM
                     LIDTKAKPPTIKVINYTNYEFVPQEDRTRIISARRIARDDAVAKSLDLTPDQVTKMRG
                     LTAQIGMVTDPADLENLKNLWTAYDAASNKPAAETALVQALTATARKSIDKTKQAAAE
                     RSAKIKAVLTDEQWKKFDAMGR"
     gene            complement(1634..2563)
                     /locus_tag="VLI90_12930"
     CDS             complement(1634..2563)
                     /locus_tag="VLI90_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV15156.1"
                     /translation="MTTEPNAAVGRPRIQRVRGGFTLVELLVVIGIIAVLIGMLLPMM
                     TRARAQANAVVCASNLRQIGVYLLQYANNNHGIIYPIGPEVPDPLPPDPSATPPFPGD
                     PNHMAFSTLGVDSNGLPPWYRWPVYVFDRAKVPPPTIVQNGSSWVVSDASKYIESGST
                     ALPPPPAPPLDQHNDYNDVPQGGLTPVWTPKIMLCPSEQDLEHVGFAHTYILNKHLEE
                     DRQQLIKYSSRAPDGRSSSDVVVMGEKKAQYADYYMERNDFATKVERYRHGIKLGSNY
                     LYLDWSVRNTPPQAAWSALDPWSFNTTPLPAGN"
     gene            3069..4646
                     /locus_tag="VLI90_12935"
     CDS             3069..4646
                     /locus_tag="VLI90_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15157.1"
                     /translation="MAQMAAAPALLAAGAALLVYAITLAGTYVYDDLAIVTLDPRVKS
                     PALWSQFWTKDYFNGGLDNLYRPLVSMSYGIQWWLHGDRPWAFHLVNWLLHAAVAAAV
                     AELTRRTVGRGAAYIAGLLFAVHPVHVEAVANIVGRAELACALGILAALTLFAKRPLT
                     FARVMGIVVCEIVAVLSKEQGILVPPMLAIYFWFVWRDDPRGAADSAAERAALRWLVI
                     SVTWCTAGYLIWREHFLRFDWDRSFLDWVLQPMVRCSPHDRVLMPLVLLGHYTQLLIC
                     PVKLSPDYGAKVIGWTVRLTDPYFGAGVAVALLWLIFTAIAIYRRRGFEAFCLLSLAL
                     SYGMIANVIAILGCNMAERWIYLPSAFFLMWLAIWIAGMPRPARLALLIAIVTLASAR
                     TFTYARRWNDRLTFYEQSLDEQPNSVQLYLLTAGELDARHDYAAADAVMARAAAVFPD
                     AWHVWMRRAVIAMDAGRLDDADRYLRRSFELSPNPMVIAVSDRLDQLKAARKAAATTP
                     SKMTPRSGSPEGARSSP"
     gene            4808..>5609
                     /locus_tag="VLI90_12940"
     CDS             4808..>5609
                     /locus_tag="VLI90_12940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV15158.1"
                     /translation="MGATDAELLNEYARSKSDAAFAALAARYGPLVYAAALRQVRRAD
                     LADDVTQAVWIVLVQRTPLAAGSHLAGWLLTTTRYCAKDALRKQARRAHHEHEAAAMK
                     PLVSPPPDATAADDDHLAKHLDEALSRLRPQASAAVALRFLQDQPLADVARATGMTVD
                     AAQKVVARALPQLRRWLTQRGVLLSSTAVLTDAMLRLPRAGNIAAPISPAAASPNSIA
                     IAKGAIHMMRLTVVKTASLATAAAFLLTAAGAGVVMMNSATAAAPPKPA"
BASE COUNT          901 a         1857 c         1784 g         1067 t
ORIGIN      
        1 gaacccggcg tggggaggat atgaaacctc ttgcgcaacc gacgcacgga tcacggggtg
       61 gtagcccgtg ccacttgtgg cggcgcgcct tgctccaccg tctctggcac gaactggaat
      121 cgatactgcg atccgcggcg gaggcgggtc agctgcacga tctgatgcgt gtgcccgacg
      181 aagtgggcgg ccgtgtcgaa tagcgcgtgt gtgccggtga cgttgaagcc ctgcaccttg
      241 cggacgcgta gcagatcagc cgggtcgagc cgggcgagca ctcggtcggc ggcggtgacg
      301 gcgtcgctca gcgtgcgccg aacggcggcg acggaggcgt ccgtcgggtc atcgaactcg
      361 ccggggcgat agcgcgtgtc cggctcgttg cccaggccgg cgcagatcca ctgctgcagg
      421 ttccccgcga gatgattggc gatgatcgcg atgcagttca tccccgccgc cggccgggcg
      481 gcgacgtcgg catcgctcag ttggtcgaga caatggttga tcttcgcgag cgcgcgggcg
      541 agcgtgtcgc gcgcttcggc gacgatggcg tgaccgacat cttggccggt gtaggtggag
      601 ccggggcctt cttgacctgc catttttcac tttatcctga gcggaggatg cgcagcatcc
      661 ggagtcgaag gatctggcgg gcgcgtcggt gaacggccga tgaacgcaaa gctaacgagt
      721 gtaaagttgc cgcgcggtgg gaagcccact tacttctcag ctatatcctt cgacttcgga
      781 tgctgcgcat ccttcgctca ggataatgct cagactcacc gccccatcgc gtcgaacttt
      841 ttccactgct cgtcggtcag caccgccttg atcttcgcgc tgcgctcggc ggcggcttgc
      901 ttggtcttgt cgatcgactt gcgggccgtc gcggtcagcg cctgcaccag cgcggtttcg
      961 gcggcgggtt tgttgcttgc cgcgtcgtac gcggtccaca ggttcttgag gttttcaagg
     1021 tcggccgggt cagtgaccat gccgatctgc gccgtcaggc cgcgcatctt cgtgacctga
     1081 tcgggcgtga ggtccaggct cttggcgacc gcgtcgtcgc gggcgattcg ccgggcggaa
     1141 atgatccgcg tgcggtcctc ctgcggaacg aattcatagt tggtgtagtt gatcaccttg
     1201 atggtaggcg gcttggcctt ggtgtcgatg agcatggccg cctcgggggc gtgcacccac
     1261 caggaggtgt cgtcctgctt gcggacggcg ggcgcggctt gggcggcggc catattattc
     1321 ccgcgcgccg acggcggcgt gcgcgtcggg gccgcgggaa tcacgccggg gtccagcagc
     1381 ttggtctcgt ggctcagcgg atcgctggtc acgtaccagt gcatgatcca accacccccg
     1441 cccaggcaca gcaggatcac cagcacgctg gcccagatgg ggatcggctg cggttctgaa
     1501 atccgcgccg ccaaggctgg ctgtgcaaca ggcgccatcg gctctgcagc aggtgccgtc
     1561 cgctccgcgt cgggtacaac tggagattga gagggttcgg tcattgttcg ttgcccagaa
     1621 atagagacga cgtttagtta ccagcgggca gcggagttgt attgaaactc cacgggtcca
     1681 gcgccgacca cgcggcttgc ggcggggtat tgcggacact ccaatccaga tacagatagt
     1741 tcgaccccag cttgattccg tgtcggtacc gttcgacctt cgtggcgaag tcgttcctct
     1801 ccatgtaata gtcggcgtat tgggccttct tttcccccat cacaaccaca tcggacgagc
     1861 tacgcccatc cggcgcccga ctggaatact tgatcagctg ctgtcgatcc tcttccaggt
     1921 gtttattcaa aatataggta tgagcgaaac cgacatgctc caagtcctgt tcagacggac
     1981 acagcatgat cttcggcgtc cacaccggcg taagcccgcc ttggggtaca tcgttgtaat
     2041 cgttgtgctg atccagtggc ggagccggcg gtggcggcaa cgccgtgctg ccagattcta
     2101 tatatttgga ggcgtcggac accacccagc tggacccatt ctggacgatg gtcggcggcg
     2161 gcaccttcgc gcggtcaaag acatacaccg gccatcgata ccacggcggc agcccgttgg
     2221 agtcaactcc caacgtgctg aaggccatgt gattcgggtc acccggaaac ggcggcgtcg
     2281 ccgaggggtc cggcggtaat ggatccggca cttccggccc aatcggataa atgatgccgt
     2341 gattgttgtt cgcgtattgc agcagatata cgccgatctg ccgcaggttc gatgcacaga
     2401 cgaccgcatt ggcctgggct ctggcgcgcg tcatcatcgg cagcagcatc ccgatgagca
     2461 ccgcgatgat gccgatcacc accagcaact cgaccagcgt aaagccaccg cgcacgcgtt
     2521 ggatccgcgg gcgacccacg gccgcgtttg gttcggttgt catgattgac gtcatacgat
     2581 tgcctcccac agccgcggcg gagccagcgc gggccacccg tgcatcttac ctcaaatatg
     2641 ctgacaaggg gcaagtattg cccgcgcggc cccgcggttc accagggcaa tcgcatttag
     2701 caaccgcgcg ctctggtccc ctctcccggt acgccgggag agggttaggg tgagggtgat
     2761 ttcgagcacc agcggctttc gaatgcaaaa tcaccctcac ctctgccctc tcccggagac
     2821 ctgcggtcag cagtaccggg agagggggaa ggagctacgt gcgattgcct tggcggttca
     2881 ccaccgccac gccaacgccc ggccagcggc cgcgcactca ccacttatgg ttattgacgg
     2941 acgagccgcc ccgttgatgc agaaattttc tgccatcggc caactcaacg gcacccggcg
     3001 gttccactac catgctgccc gtgacgtttg acgctgccgt tatcgatccg cgatcgccgc
     3061 gccgtcgcat ggcacagatg gccgccgcgc ccgcgctgct cgcggccggc gcggcgctgc
     3121 tggtgtacgc catcacgctg gccggaacgt acgtctacga cgacctcgcg atcgtcacgc
     3181 tcgatccgcg cgtcaaatcg cccgccctct ggtcgcaatt ctggacgaag gactatttca
     3241 acggcgggct cgacaacctg taccgcccgc tggtttcgat gtcgtacggc atccaatggt
     3301 ggctgcacgg ggatcggccg tgggcgtttc acctggtgaa ctggctgctg cacgccgccg
     3361 tcgccgccgc cgtggcggaa ctcacccgcc ggaccgtggg tcgcggcgcg gcgtacatcg
     3421 ccgggttgct cttcgcagtg catccggtgc acgtcgaagc ggtcgccaac atcgtcggcc
     3481 gggcggagct ggcgtgcgcg ctgggcatcc tcgctgcgct gaccctcttc gccaagcgac
     3541 ccctcacgtt cgcgcgcgtc atgggcattg tcgtttgcga aatcgtcgcg gtcctctcga
     3601 aggaacaagg catcctcgtt ccgccgatgc tggcgatcta tttctggttt gtctggcgcg
     3661 atgatccgcg cggcgcagcg gattccgcgg cagaacgcgc cgcgctgcgc tggctggtca
     3721 tcagcgtcac gtggtgcacc gccggttatc tcatctggcg cgagcatttc ctgcggttcg
     3781 attgggatcg cagctttttg gattgggtgc tgcagccgat ggtgcggtgc tcgccgcacg
     3841 atcgcgtgct catgccgctg gtcctgctgg gacattacac gcaactgttg atctgtccgg
     3901 tcaaactatc gcccgactat ggagcgaagg tgatcggctg gaccgtgcgc ctgaccgatc
     3961 catacttcgg ggccggcgtc gccgtggcgc tgctctggct gatcttcact gcaattgcaa
     4021 tctatcgccg gcgcggattt gaagccttct gcctgctctc cctcgcgctc agctatggca
     4081 tgatcgccaa cgtcatcgcc atcctcggct gcaacatggc cgagcgctgg atctacctcc
     4141 cgtcggcgtt cttcctcatg tggctggcga tttggatcgc gggcatgcct cgccccgcgc
     4201 ggctcgcgct gctgatcgcc atcgtcacgc tcgcctccgc ccgcaccttc acttacgccc
     4261 gccggtggaa cgatcggctg acgttctacg agcagtcgct ggacgagcaa ccaaattccg
     4321 tccagctgta cctgctgacc gccggcgagc tggacgcccg ccacgactac gccgcggccg
     4381 acgccgtcat ggcccgcgcc gctgcggtct tccccgacgc gtggcacgtg tggatgcgcc
     4441 gcgccgtaat cgcgatggac gccggccgcc tcgacgacgc cgatcgctac cttcgccggt
     4501 cattcgagct cagcccgaac cccatggtga tcgccgtctc cgatcgcctg gaccaactaa
     4561 aagctgcccg caaagccgcg gcgacgaccc catcgaaaat gaccccgcgt agtggtagcc
     4621 ccgaaggggc acggtcttca ccttgaaccc aaaaaaacag gtgcccccgg ggggctaccg
     4681 ccatgcaggc gcgcaaggcg caggcgctat gcgcgaagcg cgctgaacgc ggcagcgctg
     4741 tagaatcttt ggcgcccgac gaagtttttt ggcgtcctcc tctccggctg gccattctgg
     4801 gaatgaaatg ggtgcgaccg acgctgaact gctgaacgaa tacgcccggt ccaaatccga
     4861 cgcggcgttt gcggcactgg ccgcgcgcta tggcccgctg gtctacgccg ccgcgttgcg
     4921 gcaggtgcgc cgggcggacc tggccgacga cgtgacgcag gccgtctgga tcgtgctggt
     4981 ccaacggacc ccgctcgccg cagggagtca tctggctggg tggttgctga cgacgacgcg
     5041 ctactgcgcg aaggacgcgt tgcgcaagca agcccgccgc gcccatcacg aacacgaggc
     5101 cgccgcgatg aagccactcg tttcacctcc gcctgatgcc accgccgcgg acgacgatca
     5161 cctggccaag catctggacg aggcgctgtc gcgcctgcgc ccgcaggcca gcgcggccgt
     5221 cgcgctgcgc tttcttcagg atcaacctct ggccgacgtc gcccgcgcca cggggatgac
     5281 cgtcgatgcc gcccagaagg tcgtcgcccg cgcgctgccc cagctgcgac gatggctcac
     5341 ccagcgcggg gtgctgctgt ccagcaccgc ggtccttacc gatgcgatgc ttcgccttcc
     5401 gcgcgccggc aacatcgccg cgccgatcag tcccgcggcc gcttcaccga acagcattgc
     5461 cattgctaaa ggagcgatcc acatgatgcg cctgaccgtg gtcaagactg cgtccctcgc
     5521 caccgccgcg gcgtttctgt tgaccgccgc tggcgccggc gtcgtcatga tgaacagcgc
     5581 gaccgccgcc gctccaccaa aaccggcgg
//
LOCUS       DATIMH010000736         1713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1043501, whole genome shotgun sequence.
ACCESSION   DATIMH010000736 DATIMH010000000
VERSION     DATIMH010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043501"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VLI90_12945"
     CDS             complement(<1..422)
                     /locus_tag="VLI90_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HSV15159.1"
                     /translation="MARYLSEHLETVRVHVRDQKDEYKRLSERYNAQWTPTVLILDAD
                     GEERHRIEGFLPADDFLSQIALGLAKSAFQRKDYGEAERRYREIVDRFPSSEAAPEAL
                     YWAGVSNYKATGDPSALRATTTAFGQRYQDTAWAKKASV"
     gene            615..1049
                     /locus_tag="VLI90_12950"
     CDS             615..1049
                     /locus_tag="VLI90_12950"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HSV15160.1"
                     /translation="MTRTGSTTHVISIRLCRPHITIVMLVLMVSGCATRKPAVVTPPP
                     VAPRTSAEPLTFTATAYCTGHVTATGTSVSAGVVAADPAVLPMGTVIRITGAAPYDGS
                     YRVLDTGGRVRNHHVDLYIADCAAARRFGRRSVRVTVLEPAR"
     gene            complement(1058..>1713)
                     /locus_tag="VLI90_12955"
     CDS             complement(1058..>1713)
                     /locus_tag="VLI90_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSV15161.1"
                     /translation="DAGLTVANAIAFNSFLDDDSTSRAASMEKLKADMDKVAQIGGTR
                     IAATPGNNRAAVSLDNAARYYREALEMGEKAGVQPLLELWGTHPVLGPLSHGIYVSVA
                     AGRRDASLLLDVFHLYKSGTSFDSLKQINGAALHVMHLNDYPQAADSSTLNDSNRIYP
                     GDGVAPLRQIFRDMRDNGFRGYFSLELFNRDYWTKSADENLKTAMDKIRATVRSALA"
BASE COUNT          296 a          544 c          536 g          337 t
ORIGIN      
        1 accgaggcct tcttggccca ggccgtatcc tgataccgct gaccgaatgc ggtcgtcgtc
       61 gcacggagcg ccgacggatc gccggtcgcc ttgtagttgg agacaccggc ccagtagagg
      121 gcttcgggag cggcctcgct cgaaggaaat cgatccacga tctcgcgata ccgacgctca
      181 gcctcgccgt aatccttccg ttgaaaggcg gacttcgcca gaccgagcgc gatttgcgag
      241 agaaaatcat ccgccggcag aaatccttcg atgcgatgac gttcttctcc gtctgcatcc
      301 aggattagca ctgtcggcgt ccattgcgcg ttgtagcgtt cgctcaggcg cttgtactcg
      361 tccttctgat cgcgaacatg aacccgcacc gtctccaggt gttcggagag gtagcgcgcc
      421 acttgtggat ccgcatacac ctcggcttcc agccgagcac agcccgttca catcggggcg
      481 gcggtgaaat cgagcaggac gtcacgcttt gcgtcgcggg cgcgcgtcag ggcagaatcg
      541 aaatcatgag accacgtgag cgacgatgat gctgtcggca tattccctcc tcttccttcg
      601 atcctaacgc ggtcatgacg agaaccggct caaccacaca cgtcatctcg atccggctct
      661 gccgtccgca catcacgatc gtgatgttgg tgctgatggt atcgggctgc gcgacacgga
      721 aacccgcggt tgtgacgccg ccaccggtgg cgccgcgcac atcggcggag cccctgacgt
      781 tcacggccac cgcgtactgc acggggcacg tcacggcaac agggacgtca gtgtctgcgg
      841 gtgtcgttgc ggcggatccg gccgtgctac cgatgggaac ggtgattcgg atcaccggcg
      901 ccgcgccgta tgacggctcg tatcgcgtgc tcgataccgg cgggcgcgtg cgcaatcacc
      961 acgtggatct ctacattgcc gattgcgccg cggcgcgccg attcggccgg cggtcggttc
     1021 gggtgaccgt ccttgaaccg gcgcgctgaa tcactggcta cgcgagcgcg gagcgaaccg
     1081 ttgcccggat cttgtccatc gctgtcttca ggttttcgtc cgcgctcttg gtccagtagt
     1141 cgcggttgaa cagctcgagc gagaagtagc cgcggaaccc gttgtcgcgc atgtcgcgga
     1201 agatctggcg caacggcgcg acaccgtctc ccggatagat gcggttgctg tcgttgaggg
     1261 tggatgaatc ggccgcctgc ggataatcat tcaggtgcat gacgtgcagc gccgccccgt
     1321 tgatctgctt caacgagtcg aaggacgttc cgctcttata caggtggaac acgtcaagca
     1381 ggaggctggc gtcgcgacgc ccggcggcca cgctgacata aattccgtgc gagagcggcc
     1441 cgagcaccgg atgcgtgccc cacaattcga gcagcggctg cacgccggcc ttttcaccca
     1501 tctcgagcgc ctcgcgataa tagcgggcgg cgttgtccag gctcacagcg gcgcggttgt
     1561 tgcccggcgt cgcggcgatc cgcgtaccgc cgatctgcgc caccttgtcc atgtcggcct
     1621 tgagtttttc catcgacgcg gcacggctcg tgctgtcgtc atcgagaaat gaattgaacg
     1681 caatcgcatt ggcaaccgtg agtcctgcgt ccg
//
LOCUS       DATIMH010000737         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1622502, whole genome shotgun sequence.
ACCESSION   DATIMH010000737 DATIMH010000000
VERSION     DATIMH010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622502"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VLI90_12960"
     CDS             complement(<1..1551)
                     /locus_tag="VLI90_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV15162.1"
                     /translation="MAQSNDQTLLQQYAADGSQDAFAQLVDRHIDLVHTAARRMVHGD
                     SHLAEDVTQAVFILLARRAGQIKPNVVLAGWLYNATRLSASVALRGQRRRQRHETAAA
                     SEALRMRSSNSSQRAGDSPAPSWDQLETVIDDAMGKLSTADRDAIALRFFQNKTMAEA
                     GAAMGITEDAAKMRVTRAVTRLRNTLARAGVTAAPVPALMVVMSAAVPNAAPAGLAQA
                     TMAAALAAIGTAAGKAGGSYAIAKGTSAMMTWIKIKLTLATAAVVMAVVGTGAAVVAQ
                     TLLKAHEAPSPAVQVVAATPSTPAIPIATPAGAEATAPPAPLTLNLDGVDPRTAIAMI
                     AKISGTKIDIWPDTAWNPQFMGFGKAMPKVTLHADNQPFWSVLRDFCQQAGARVQEMG
                     NRANTVTIAADDNGSFSRLPAFVGPTHMVVLNSISRSNSVRFDQDRSIHEDRGGSFDV
                     WIDPRVNVLKASHGVWIESAVDENGKSLVVPHDPAWDEFYMGGFSPWHLSLNFPLNYD
                     WTQSKTLKQ"
     gene            complement(1935..>2839)
                     /locus_tag="VLI90_12965"
     CDS             complement(1935..>2839)
                     /locus_tag="VLI90_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HSV15163.1"
                     /translation="DIINGQDPIVAFLYDTKKGHCEYFAGAMTLLCQSLGIPARMVVG
                     FKCDEYNDFGNYYNVRQSHAHAWVEVLTDSGGWQTFDPTSGHEAAAATLNPWQKTRHF
                     FEYLEYTWANAVIAYNAENRETLVNSLDNRITNTAIASSVAMGGVKGLFDYIASWLAG
                     HVLGPFMALLAIASIGAIGWFLAERWRLRRRASRIGLDSLPPSAQARLVRQLAFYDDL
                     LRLLERHQIQRPRHLTPLEFSNSLSFLPSEIYDTIRRLTSLFYRIRYGHADLDPGQQR
                     RLGNVITRLADVMPKPAPVKAPAM"
BASE COUNT          449 a          919 c          940 g          531 t
ORIGIN      
        1 ctgcttcagc gtcttgcttt gagtccagtc gtagttcagc gggaagttca ggctcaggtg
       61 ccacggcgaa aatccgccca tgtagaattc gtcccacgcc ggatcgtgcg gcaccaccag
      121 cgatttgccg ttctcgtcca cggccgactc gatccacacg ccgtggctgg ccttcagcac
      181 gttcacgcgc gggtcgatcc agacgtcgaa gctgccgccg cggtcttcgt ggatgctgcg
      241 gtcctgatcg aagcgcacgc tgttgctgcg cgagatcgag ttgagcacga ccatgtgcgt
      301 cggtccgacg aacgccggca atcggctgaa cgagccgttg tcgtcggcgg cgatcgtgac
      361 cgtgttcgcg cggttgccca tctcctgcac gcgcgcgcct gcctgctgac agaaatcgcg
      421 caggacggac cagaacggtt gattgtccgc gtgcagcgtc accttgggca ttgctttgcc
      481 gaagcccatg aactgcgggt tccacgcggt gtcgggccag atgtcgatct tggtgccgct
      541 gatcttggcg atcatggcga tcgcggtgcg cggatcgacg ccgtccaggt tcagcgtcaa
      601 cggcgccggt ggcgccgttg cctcagcacc ggccggcgtt gcaattggaa tcgcgggcgt
      661 gctcggcgtc gccgccacca cctgtaccgc cggtgatggc gcctcgtgcg ccttcagtag
      721 cgtttgcgcg accaccgccg cgcccgtgcc gacgacggcc atcaccaccg ccgccgtcgc
      781 gagggtcaac ttgatcttga tccacgtcat catcgcgctc gttcctttcg cgattgcgta
      841 agaacccccg gccttccccg cggccgtgcc gatcgccgcc agcgcggcgg ccatggtggc
      901 ttgcgccaat cccgccggcg ccgcgttcgg gacggcggcg gacatcacca ccatcagcgc
      961 cggcaccggc gccgccgtca cgcccgcgcg ggccaaggtg tttcgcaggc gggtgaccgc
     1021 ccgggtcacg cgcatcttcg cggcgtcttc ggtaatgccc atggccgccc ccgcctcggc
     1081 catggtcttg ttctggaaga agcgcagcgc gatcgcatcg cgatccgcgg tggacagctt
     1141 gcccatcgcg tcgtcgatca ccgtttcgag ttggtcccac gatggcgcag gagaatcgcc
     1201 cgcacgttgg ctgctattgc tgctgcgcat gcgaagggcc tccgacgcgg cggcggtttc
     1261 gtggcgctgc cggcggcgct ggccgcgcag cgcaaccgac gccgacaacc gcgtcgcgtt
     1321 gtagagccac cccgccagca cgacattcgg cttgatctgg ccagcgcgcc gggccagcaa
     1381 gatgaacacc gcctgcgtga cgtcttcagc caagtgggaa tcgccatgca ccattcgccg
     1441 cgcggcggtg tgcaccaggt cgatgtggcg gtccaccaac tgcgcgaacg cgtcctgcga
     1501 gccgtcggcg gcatattgct ggagaagggt ttgatcgttt gattgcgcca tcccgggcta
     1561 tcctcgcgtt cgccctatca tgtccggcgg cgggcgaagg taacaggcgg gggtgtgtca
     1621 tcctggagcg caggcgtact cgccggagtc gaaggatctc gccgaggagt cgatgaatcc
     1681 ttgatgaggg aagacggtgc gcagtcgctt gcggtttcgc agacggacaa aacgcggtaa
     1741 ctggtctgcg aaaccgaaag cgactgctgt cccgttcctg tcattcaacg ttcactcact
     1801 tctccgctag atccttcgac tgcgcacgcg tacgtgcttc gctcaggatg acgcgcgcta
     1861 aggcgacatc cgggctgtcg ggcgcgctaa gacatccggg tcgtcgggag taccctccga
     1921 acccggcgtg gggtttacat cgcgggcgcc ttcaccggcg cgggcttcgg catcacatcc
     1981 gccagtcgcg tgatcacgtt gcccaggcgg cgttgctggc cggggtccag gtccgcgtgg
     2041 ccgtagcgga tgcggtagaa cagcgacgtt agccgccgga tcgtgtcata gatttcgctc
     2101 ggcaggaagc tgagggagtt gctgaactcc agcggcgtca ggtgccgcgg gcgctggatc
     2161 tggtggcgct cgagcaggcg aagcaggtcg tcgtaaaagg cgagttgccg cacgagcctt
     2221 gcctgcgcgg agggtggcag ggaatcgaga ccgatccggc tggcccggcg gcgcaggcgc
     2281 cagcgctcgg ccaggaacca tccaatcgcg ccgatcgacg caatggcgag cagcgccatg
     2341 aatggtccaa gcacatgacc ggcgagccag ctggcgatgt agtcgaacaa ccccttcaca
     2401 ccgcccatgg cgacggagct ggcaatggcg gtgttggtga tgcggttgtc tagcgaattg
     2461 accagcgtct cgcggttctc ggcgttgtac gcgatgaccg cgttcgccca ggtgtattcc
     2521 agatattcga aaaaatgccg cgtcttctgc cagggattca gcgttgccgc ggcggcctcg
     2581 tggccgctgg tcgggtcgaa cgtctgccag ccgcccgaat ccgtcagcac ttccacccac
     2641 gcgtgggcgt gcgactggcg gacgttgtag tagttgccga agtcgttgta ctcgtcacat
     2701 ttgaacccga cgaccatgcg tgcggggatg cccaggctct ggcacagcag cgtcatggcg
     2761 ccggcgaaat attcgcagtg gcctttcttc gtgtcgtaca ggaacgcgac gatcggatct
     2821 tgcccgttga tgatgtcgc
//
LOCUS       DATIMH010000738         4712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3351566, whole genome shotgun sequence.
ACCESSION   DATIMH010000738 DATIMH010000000
VERSION     DATIMH010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3351566"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VLI90_12970"
     CDS             <1..921
                     /locus_tag="VLI90_12970"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HSV15164.1"
                     /translation="MITSITPERLASSVLAVPPLCRNADLTLNEAENAKFIRYLEAGG
                     IRTLLYGGNANFYHIALSEYEQVLSCLEQSAAADTLVIPSVAATFGTMMDQAKIVRKH
                     RFPTVMILPLQGAVTSKGVEAGIRRFVDAAGIPALLYLKNDGYIEVQEVRKLAESKTI
                     SGIKYAIVRDDPANDPYLRKLCDQVDRKLIVSGLGEQPAIIHMRDVGLPGFTAGVVCV
                     APKLTAAMLKAIRAGDFAKAEKIREVCKPLEDHRNAINPVRVLHEAVRLAGIANTGPL
                     LPLLSNLEEAHHARVRDAAVNLLEAERQLA"
     gene            1026..2732
                     /locus_tag="VLI90_12975"
     CDS             1026..2732
                     /locus_tag="VLI90_12975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV15165.1"
                     /translation="MDTSIAPAPLRIRDYLLIGVFAAVLFGYAGVGGRPLTMHEARLP
                     QTSREMLATHRWLLPHSGIRPWLERPPLPHWIVIGSMKLFGHDDRVWVVRLPSAIMGV
                     IIVLVVGWMSGRWFGRTIGVVAALVLATSFEFYSYASLAEDDIYLGALVTICVAFFAS
                     AEFFSPAATADRRLNVLVGRPWQVGLFFITLGLTNLTKGPLLGILIVGSSVGSYLLWN
                     AIADGAGRALLRYVWLWGWLATIALTLAWPAWAYHVYPDVLANWKYDYLGRMSGAYTD
                     INQGWYYYAPALAAAMAPWTPACLIGLIALTMGLRHSNERGGLREETGSSGIPSDPAS
                     VGALSNPSARAAQFVLCWAILPLIVLSIPKGKHHHYLVPFLAPWAILAAFGVVEIAAY
                     FRWSRRTGPVVFTVILSLLLVGYCCGETFLAASTDHTVADTAFLRRVRQEVPPGSPLF
                     INAKLGPVGNLDFFRIQFYSRPDAMLLHNLSYLRDENIKASEVYVITRARDEEKLKQL
                     GTPTLIDQSESSHEVTTPAGRFSLFKLVFDPNLKREAAPTHITNMQAMEREPGPWCGP
                     AL"
     gene            complement(2834..3514)
                     /gene="rpiA"
                     /locus_tag="VLI90_12980"
     CDS             complement(2834..3514)
                     /gene="rpiA"
                     /locus_tag="VLI90_12980"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HSV15166.1"
                     /translation="MTPKQRAAEAALPFIQSGMVIGLGTGSTADFFLQALAAALKTGQ
                     LRDIAGVPTSRQSEHRAMELGIPLTTLLRSPVLDVTVDGADEIAPNLDVIKGLGGALT
                     REKIVAQNSKQLVIIADTSKTVTTLGTKSPLPVEVLPFAHESHEPFIRSLGAEPTLRK
                     VGGMIFNTDNGNLIYDCRFSQIEDPIGVERALRKRAGIVGTGLFLGLATSVLIADERE
                     TIRRDRAT"
     gene            3771..4364
                     /locus_tag="VLI90_12985"
     CDS             3771..4364
                     /locus_tag="VLI90_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15167.1"
                     /translation="MLLQTNSYIVPKDKKSEHARVIRRFRQTLLRLGCDHFEVYEQVG
                     ANWSNSESTGRYVQILRFVDRKHQLAVQAAERSDPTAQAVIAEFCDLINFPYQQQQGL
                     FAVGFYTSVLPMAPKRGQRTELISEDETVGAEPAAEGGAPAASADAEAAGAGDEGDLE
                     GLVGGDAAEETDLSEGGSGTEAFIEGEQVKHHEERDA"
BASE COUNT          832 a         1373 c         1568 g          939 t
ORIGIN      
        1 atgatcacat cgataacgcc cgaacgtctc gcctcatcgg tcctggcggt gccgccgctt
       61 tgtcgcaacg cggacctcac gctgaacgaa gcggagaacg cgaagttcat ccgctatctc
      121 gaagccggcg gtattcgcac gttgctgtat ggcggcaacg cgaactttta tcacatcgcg
      181 ctgagcgagt atgaacaggt gctgagctgt ctcgagcaat cggccgcggc ggacacgctg
      241 gtcattccat ccgtcgccgc gacgttcggc acgatgatgg accaggcgaa gatcgtgcgg
      301 aagcaccgat ttccgacggt gatgatcctg ccgctgcagg gggcggtgac cagcaagggc
      361 gtcgaagccg gcatccggcg gtttgtggac gcggcgggga ttccggcgct gttgtacctg
      421 aagaacgacg gctatatcga agtgcaagag gtgcgcaagc tggcggagtc gaagacgatc
      481 agcggcatca agtacgcgat cgtgcgcgat gacccggcga atgatcctta cctgcgaaag
      541 ctatgcgatc aggtcgatcg caagctgatc gtcagcgggc tcggcgagca accggcgatc
      601 attcacatgc gtgacgtcgg cctgcccggg ttcaccgcgg gcgtcgtctg cgtcgcgcca
      661 aaactgacgg cggcgatgtt gaaagcgatc cgcgctggcg acttcgccaa ggccgaaaaa
      721 attcgcgagg tgtgcaagcc gctggaagac catcgcaacg cgatcaaccc ggtgcgcgtg
      781 ctgcacgaag cggtgcgact ggccggcatc gccaacaccg ggccgctgct gccgctgctg
      841 agcaaccttg aagaggcgca tcatgcccgc gtgcgcgatg cggcggtcaa tctgctggag
      901 gcggagcggc agttggcgta ggtttgatca atgcagaatg aatccagaga agtgttcttc
      961 attctgcatt ctgcatctcc ccggattgca tccccctcac ccttcgtgtg caatgccgcg
     1021 acttcatgga cacctcgatc gcgcctgcgc ccctgcgaat tcgtgattac ctgctgatcg
     1081 gcgtcttcgc cgcggtattg ttcgggtacg ccggtgtcgg cgggcggccg ctgacgatgc
     1141 atgaagcgcg gctgccgcag acgtcgcgcg agatgctggc gacgcaccgc tggctgctgc
     1201 cgcacagcgg gattcggccg tggctggagc gcccgccgct gccgcactgg atcgtcatcg
     1261 ggtcgatgaa gctgttcggc cacgacgatc gcgtgtgggt cgtccgcctg ccgtcggcga
     1321 tcatgggcgt catcatcgtg ttggtggtcg ggtggatgtc gggacggtgg ttcggtcgga
     1381 cgatcggcgt cgtcgcggcg ctggtgttgg cgacgagctt cgagttttat tcttacgcga
     1441 gcctggccga ggacgacatt tatctgggcg cgctggtgac gatctgcgtc gcgttcttcg
     1501 ccagcgccga attcttctcg ccggcggcga cggcggatcg tcggttgaat gtcctggtcg
     1561 gccggccgtg gcaggtcggg ctgttcttca tcacgcttgg gctgacgaac ctgacgaagg
     1621 ggccgctgct ggggatcctg atcgtcggtt ctagcgtggg ttcgtacttg ctgtggaatg
     1681 cgatcgccga tggcgcgggg cgcgccctgt tgcgctatgt gtggctgtgg ggctggctgg
     1741 cgacgatcgc gctgacgctg gcgtggccgg cgtgggcgta tcacgtttat cccgacgtgc
     1801 tggcgaactg gaaatatgat tatctcggcc gcatgagcgg ggcgtatacc gacatcaacc
     1861 agggttggta ttactacgcc cccgcgctgg cggcggcgat ggcgccgtgg acgccggctt
     1921 gtttgattgg gctgatcgcg ctgacgatgg ggctgcggca ttccaatgaa cggggtggct
     1981 tgcgcgaaga aaccgggtct tcgggcatac cctcagaccc ggcgtcggtg ggcgcattat
     2041 cgaatccatc agcgcgcgcc gctcaattcg ttttgtgctg ggcgatcctg ccgctgatcg
     2101 tgctatcgat cccaaagggc aagcaccacc attatctcgt cccgttcctc gcgccctggg
     2161 cgatcctcgc cgcgttcggc gtggtcgaaa tcgcggcgta cttccggtgg tcgcgccgta
     2221 cggggccggt tgtcttcacg gtgatcctca gcttgctact ggttggctac tgctgcggtg
     2281 aaacctttct cgccgcttcg acggatcaca cggtggcgga caccgcgttc cttcggcggg
     2341 tccgccagga ggtgccgccg gggtcgccgc tgtttatcaa cgccaagctc ggtcccgtcg
     2401 gcaacctcga cttcttccgc atccagtttt attcgcggcc cgacgcgatg ctgctgcaca
     2461 acctgagcta cctgcgcgat gaaaatatta aagccagtga agtgtatgtc atcacgcggg
     2521 cgcgcgacga ggagaagctg aagcagctgg gcacgccgac gctgatcgat caaagcgaaa
     2581 gttcgcacga ggtgacaacg ccggcggggc ggttctcgct gttcaagctg gtgttcgacc
     2641 cgaacctgaa gcgggaggcg gcgccgacgc acattaccaa catgcaggcg atggagcgcg
     2701 agcccgggcc atggtgcgga ccggcgctgt gaggtttgtg gaagaccttc aaaagcccgc
     2761 ggctctgccg tgggacggtt ttcgaaattc cggtctcacg gcagagccgt gcgctttgtc
     2821 tagggtgact tatttacgtc gcgcgatcgc gccggatcgt ctcgcgctca tccgcaatca
     2881 acactgacgt cgccaatcct aagaaaagtc cggtgcctac gatgccggcg cgcttgcgta
     2941 gggcgcgctc gacgccgatc ggatcttcga tctggctgaa ccggcaatcg taaatcaggt
     3001 tgccgttgtc ggtgttgaag atcatgccgc cgactttgcg cagcgtgggc tcggcgccga
     3061 gcgatcggat gaacggctcg tgcgactcgt gcgcgaacgg cagcacttcc accggcaggg
     3121 gggacttggt gccgagcgtc gtcactgtct tgctcgtgtc ggcgatgatg accagttgct
     3181 tggagttttg cgcgacgatc ttctcgcgcg tcagcgcgcc gccgaggccc ttgatgacgt
     3241 ccaggttcgg ggcgatctca tcggcgccgt ccacggtgac gtccagcacc ggcgagcgca
     3301 gcagcgtggt cagcgggatg cccagctcca tcgcgcggtg ttcggattgg cgcgacgtgg
     3361 gcacgccggc gatgtcgcga agctggccgg tcttcagggc ggcggccagt gcctgcagaa
     3421 agaagtccgc ggtggaaccg gtgcccaggc cgatcaccat gccgctttga atgaacggaa
     3481 gggcggcctc ggccgcccgc tgtttgggag tcatcgtggg tcggaagata tatctggacg
     3541 tggggaaatg cgaatcgtca tcgttgccgc ttacggcttg gcgatcttcg cccattggat
     3601 tgccaagccg ccagcggcga acttatcaga ccatgtccga aatcataagc aatccctgca
     3661 ctgcggcgga gaacaacctg acgcgccggg cgttcaattg tttgaatggc tcgtatggtt
     3721 gtggcacaat agacgcttag ccggttcacc acggttcata ggacgcccgc atgcttttgc
     3781 agacgaacag ttacatcgtc cccaaggata agaagtcgga acacgcccgc gtgatccggc
     3841 ggtttcgcca gacgctgctg cggctggggt gcgatcactt cgaagtctac gaacaggtgg
     3901 gcgcgaactg gagcaacagc gaatcgaccg ggcgatacgt tcagatcctg cgcttcgtcg
     3961 atcgcaagca tcagctggcc gtccaggccg ccgagcgcag cgatccaacc gcgcaggcag
     4021 tgatcgcgga gttctgtgat ctgattaact ttccgtatca gcagcagcag ggtttgttcg
     4081 ccgtcggatt ctacaccagc gttctgccga tggcgcccaa gcgcgggcag cgtacggagc
     4141 tgatcagcga agacgagacg gttggcgccg agccagcggc tgaaggcggc gcgcccgccg
     4201 cgagcgccga cgccgaagcg gcgggcgctg gggacgaagg cgaccttgaa ggcttggtcg
     4261 gcggcgatgc cgcggaagag acagatctgt ccgaaggcgg cagcggcact gaagccttca
     4321 tcgaaggtga gcaagtcaaa catcacgagg aacgtgacgc gtaacacgta atccgtaacg
     4381 ggtcatgcat aagaggtgta gcgaagcttc tggtgttacg cgttacgaac tgcgcattac
     4441 gactccaccg cacaattccc ggaattttat tgacgtttcg cccgacttct gtatctttta
     4501 atccgtccgt cctgaggcac gtctcgaagg tgtaaaacgt cgcgggttcc catatcgccc
     4561 gcgattgatg gaaaacgaat gagcggttga tcgccagatg gagccggcga tgcatttggg
     4621 tgattcggga tcttcctgtt cgcgcgcggg ctgagtcgcg cgcacgcggg aagacggcat
     4681 gtcgatggtt gacgccatgt caacgcttga ca
//
LOCUS       DATIMH010000739         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1622987, whole genome shotgun sequence.
ACCESSION   DATIMH010000739 DATIMH010000000
VERSION     DATIMH010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622987"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1098)
                     /locus_tag="VLI90_12990"
     CDS             complement(46..1098)
                     /locus_tag="VLI90_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15168.1"
                     /translation="MNELLDRRRFLSATSASLTAAALASITPRLLRAAPTTAPTTAAA
                     MPKSPLFRDPIFDGPTDPTIIYNRDARQWWMFYTARRANVDTEGAAWVHGTDIGIASS
                     ADGGRSWLYRGIAQGLNVQPGRNTYWAPEIIWDNGVYHMYVSYITGVPTAWTGDRDIL
                     HYVSSNLWDWEMVGPLSLSSHRVIDACVWPLPAGGWRMWYKDEANKSHIYAADSANLM
                     EWNVVGPVITNRGQEGPNVFRHAGAYWMVTDMWSGLFVYRSADLTTWKHQPTVLLATL
                     GTRAGDNAIGHHAFMLSQGDDAFIFYFTEPDKRHARVQVAALDVQDDQLTCDRDRAFA
                     MDLSPEKTATLRGGSS"
     gene            complement(1104..>3067)
                     /locus_tag="VLI90_12995"
     CDS             complement(1104..>3067)
                     /locus_tag="VLI90_12995"
                     /inference="COORDINATES: protein motif:HMM:NF026034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HSV15169.1"
                     /translation="MTVGCAAPRTESAPVTSTQPTTAPSTQPSAVTVSDDGETYTLSN
                     GIVTARILKHSGAMASLVYQNIETLAGNLGRPTGNWSHSAASDVVIDAITIDPKANSG
                     QRGEVSIKGVSNGHPMGQGPGGSMVADIEIRYALDRGSSGIYTYCTFTHQPYYPAMSV
                     GEARFYVKLNDDVYDWMTVDANRNMKMITASDWNHGTQMNMKEARLMNTGIDKGQVEH
                     KYDYSANQFDVTAWGWSSTTRHVGFWFINPSVEYLSGGPTKMELSAHRDATFNLGPEY
                     FDAPAAPCLLNYWRSSHYGGSVLSVAVNERWEKVVGPFLIYCNAGASPDAMWKDALAR
                     SKKESAAWPYNWVNGVDYPHADLRGDVKGQLALNDPQAKSAKLPNLLVGLTAPDYTVN
                     GRFGQMTVDWQTDAKHYEFWTRGDADGKFLMLNVRPGTYTLHAIADGVLGDFARANVT
                     VLPDQGLDLGKIEWRPVRHGRQLWDIGIPNRSAKEFRHGDDYWHWGLYLQYPKEFPND
                     VNYTIGQSDFHKDWNYCQVPRATDALGKSQGTATTWTVHFNLPDAPPPGKAILRLAFC
                     GSSNTTVSVTVNGKPAGDTGRLPYNATINRDAVQGNWFERDVTFDTSLMHAGENDLKL
                     SIPAGGVTSGVEYDYVRLELADGPATTQK"
BASE COUNT          509 a          995 c          957 g          606 t
ORIGIN      
        1 agatccttcg acggcgcacg agtacgtgct gcgcttgagg ataaatcacg aactcccgcc
       61 ccgcagcgtc gccgtcttct ccggcgacag gtccatcgca aacgcccgat cgcgatcgca
      121 cgtcaactgg tcatcctgca catccagcgc cgcgacctga acccgcgcgt gccgcttgtc
      181 cggctccgtg aaatagaaga tgaacgcatc atccccctgc gacaacatga acgcgtgatg
      241 gccgatcgcg ttgtcgccgg cgcgcgtgcc cagcgtggcc agcagcacgg tcggctgatg
      301 cttccacgtc gttagatccg ccgagcgata gacgaacagc cccgaccaca tgtcggtcac
      361 catccaatac gccccggcgt ggcggaagac gttcgggcct tcctgtccgc ggttggtgat
      421 gaccggtccg acgacgttcc attccatcag gttcgcgctg tcggcggcgt agatgtggct
      481 tttgttcgct tcgtccttgt accacatgcg ccagccgccg gccggcagcg gccacacgca
      541 cgcgtcgatc acgcgatggc tcgacagcga cagcggcccg accatctccc aatcccaaag
      601 gttgctcgac acgtagtgca ggatgtcgcg gtcgccggtc cacgcagtcg gcacgccggt
      661 gatgtacgac acgtacatgt gatacacccc gttgtcccag atgatctccg gtgcccaata
      721 ggtgttccgg cccggctgca cgttcagccc ctgcgcgatg ccgcggtaga gccacgatcg
      781 cccgccatcg gcgctgctgg cgatgccgat gtcggtgcca tgcacccacg cggcgccttc
      841 ggtgtccacg ttcgcccgcc gggcggtgta gaacatccac cactgcctgg cgtcgcggtt
      901 gtagatgatc gtgggatcgg tgggaccgtc gaagatcgga tcgcgaaaga gcggcgattt
      961 gggcattgcc gcggccgtcg tcggcgcggt ggtgggggct gcgcgaagca gccgtggtgt
     1021 gatgctcgcc agcgcggccg cggtcagtga cgcggaggtc gcgctgagaa accgccgtcg
     1081 atcgagcagt tcattcatgg cgttcacttc tgcgtcgtcg ccgggccatc ggccagctcc
     1141 agccgcacgt agtcgtattc gacgccgctc gtcacaccac cggcggggat gctcagcttc
     1201 aaatcgttct cgcccgcgtg catgagggac gtgtcaaacg tcacgtcgcg ctcgaaccag
     1261 ttgccctgca ccgcgtctcg gttgatcgtc gcgttgtacg gcaatcgccc ggtgtcaccg
     1321 gcgggtttgc cgttgactgt caccgatacg gttgtattag aagacccgca aaacgccagc
     1381 cgcaggatgg ccttgcccgg cgggggggcg tcgggcaggt tgaagtgtac cgtccacgtc
     1441 gttgccgttc cctgggattt gcccaacgca tccgtcgcgc gcggcacctg gcaatagttc
     1501 cagtccttgt ggaaatcgct ctggccgatc gtgtagttca cgtcgttggg aaattccttc
     1561 ggatactgca ggtacagccc ccagtgccaa tagtcatcgc cgtggcggaa ttccttggcc
     1621 gatcgattgg gaatgccgat gtcccacagc tgccggccgt ggcgcacggg ccgccattca
     1681 atcttgccca ggtccagtcc ctggtccggc aggaccgtca cgttcgcccg cgcgaaatcg
     1741 cccagcaccc cgtcggcaat cgcgtgcagc gtgtacgtgc cgggacgcac gttgagcatc
     1801 aggaactttc cgtcagcgtc gccgcgtgtc cagaattcat aatgcttcgc gtccgtttgc
     1861 cagtcgaccg tcatctgccc gaaccggcca tttaccgtgt aatccggcgc ggtcagtccc
     1921 acgagcaggt tgggcagctt tgccgatttg gcttgcgggt cgttcagcgc cagctgaccc
     1981 ttcacatcac cgcgcaggtc cgcgtgcggg taatccacgc cgttcaccca gttgtacggc
     2041 cacgccgccg attccttctt cgatcgcgcc agcgcatcct tccacatcgc atccggcgac
     2101 gcgccggcgt tgcaatagat caggaacggc ccgacgactt tctcccaccg ctcgttgacg
     2161 gccaccgaca gcacgctgcc gccgtaatgg ctcgaccgcc aatagttcag caggcacggc
     2221 gccgccgggg catcgaaata ttccgggccg aggttgaacg tcgcatcacg atgggcggac
     2281 agctccatct tggtcggccc accgctcaaa tattcaaccg acggattgat gaaccagaag
     2341 ccgacgtgcc gcgtggtgct ggaccaaccc cacgcggtga cgtcgaactg gttggccgag
     2401 taatcgtact tgtgctcgac ctgtcccttg tcgatcccgg tgttcatcaa ccgggcttcc
     2461 ttcatgttca tctgcgtgcc gtggttccag tccgacgcgg tgatcatctt catgttgcgg
     2521 ttggcgtcga cggtcatcca gtcgtagacg tcgtcattca gcttcacgta gaagcgcgct
     2581 tcgcccacgc tcatcgcggg gtagtagggc tggtgcgtaa aagtgcaata cgtgtagatg
     2641 ccactactgc cgcggtccag cgcgtagcgg atctcgatat ccgccaccat gctgccgccg
     2701 ggtccttggc ccatgggatg accgttggag acgcccttga tcgagacttc cccgcgttgg
     2761 ccgctgttgg ctttcggatc gatggtgatc gcatcgatga cgacatcaga cgcggccgag
     2821 tgcgaccagt tgccggtggg gcggccgagg ttgccggcga gcgtttcgat gttctggtaa
     2881 acgagcgacg ccatcgcgcc gctgtgcttg aggatccgcg cggtgacgat gccgttggag
     2941 agcgtgtagg tctcgccgtc gtcggagacg gtgacggcgg aaggttgagt ggacggggcg
     3001 gtggtaggct gcgtgctggt gaccggcgca gattccgtgc gaggcgcggc acaaccgacc
     3061 gtcataa
//
LOCUS       DATIMH010000740         1643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2196826, whole genome shotgun sequence.
ACCESSION   DATIMH010000740 DATIMH010000000
VERSION     DATIMH010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196826"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VLI90_13000"
     CDS             complement(<1..314)
                     /locus_tag="VLI90_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15170.1"
                     /translation="MIYRIDVRTTPTARGGQAAMDPVGQSIRHQIQEFGPSVGPISTS
                     RIFLIDTGAPRADVQRVAKELLADPIVETAELVEGGTTDFNGDTSRIEIHLKPGVMDP
                     VAA"
     gene            480..>1643
                     /locus_tag="VLI90_13005"
     CDS             480..>1643
                     /locus_tag="VLI90_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSV15171.1"
                     /translation="MWCRFVPPCAAGALVICAITPRASAIISSNPAPYQGVTINTFIG
                     ADRFYDAGFLGQSAVVANIEGGFVWNQHETLTQVTTFFNDPSITGQFDAHATAVGQTI
                     AGEGSLFYHQAGIAPFANLWSGAIATGFGPGGSFFLSDASFATPYLMAMKTGVNGLGT
                     ADVINSSWGDASELDGNGLYTLTIDALAAATGKTVVLAAGNSGPGADTVGSPASGFNA
                     IVVGALGSDTSNPQYGTPSNFSSRGPNDVFIPIAADGSAGVTLKAKRAPVDLAAPGEN
                     LIVAYYGGATGSNSGGIDTSNGATNFYSFNAAGTSFAAPIVAGGAALLVDAAHSYFPN
                     DDKAVDGRVIKAVLMNAADKTVGWDNGQSLVGGVVRTTQALDYATGAGRVDLSK"
BASE COUNT          274 a          508 c          556 g          305 t
ORIGIN      
        1 gccgccaccg ggtccatcac gccgggcttg aggtggattt cgatccggct cgtgtcgccg
       61 ttgaaatcgg tcgtgccgcc ttcgacaagc tcggccgttt ccacgatcgg gtcggccagc
      121 aattccttcg caacgcgctg cacgtcagct cgtggcgcgc cggtgtcgat caggaagatc
      181 cgcgacgtgc tgatcggacc aacggaaggg ccgaattcct gaatctgatg acggatggat
      241 tgcccgacag ggtccatcgc ggcttggccg ccgcgtgcgg tgggggtggt gcggacgtcg
      301 atacggtaga tcatgcgggg attctagcga tgccggatcg accctcaacc acccggcgag
      361 ctcgacatta tcggtcacta acgtgcgcgc tgatgcgaat cgaaaaaacc gtggtatagt
      421 cgatgcacct gccgccgtgg gtgtcctttc ttttggcggt ggagtgggag ccgttttcga
      481 tgtggtgccg ttttgtgccg ccgtgcgcgg cgggcgcgct ggtgatctgt gcgatcacgc
      541 cgcgcgcaag cgcgatcatt tccagcaatc ctgcgcccta ccagggcgtc acgatcaaca
      601 cgtttattgg tgccgaccgc ttttacgatg ccgggtttct cggccagagt gccgtcgtcg
      661 ccaacatcga aggcggcttc gtctggaacc agcacgagac gctgacgcag gtgacgacgt
      721 tcttcaatga cccgtcgatc accgggcagt tcgacgcgca cgcgacagcc gtcgggcaga
      781 cgatcgccgg ggaaggatcg ctgttctatc accaggcggg gatcgcgccg tttgcgaacc
      841 tgtggtcggg cgcgattgcc acgggctttg gtcctggcgg cagtttcttt ctgtctgatg
      901 cctcgttcgc cacgccgtac ctgatggcga tgaagaccgg cgtgaacggc ctggggaccg
      961 ccgatgtcat caacagcagc tggggcgatg cgagcgagct ggacggcaac ggcttgtata
     1021 cgctgacgat cgacgcgctg gccgccgcga ccggcaagac cgtggtgctg gctgcgggga
     1081 acagcggtcc cggcgcggac acggtgggat cgccggcgtc ggggttcaac gcgatcgtcg
     1141 tcggcgcgct cggcagcgac acgtcgaatc cgcaatatgg cacgccatcg aacttttcat
     1201 cgcgcgggcc caacgacgtc ttcatcccga tcgccgccga tggcagcgcc ggcgttacgc
     1261 tgaaggcgaa gcgcgcgccg gtggatctcg ccgcgccggg cgagaacctg atcgtcgcgt
     1321 actacggcgg ggcgaccgga tcgaacagcg gcgggattga cacgagcaac ggagcgacga
     1381 acttttattc cttcaacgcc gccggcacca gcttcgccgc gccgatcgtc gccggcgggg
     1441 cggcgctgct ggtcgatgcg gctcactcgt attttcccaa tgacgacaag gccgttgatg
     1501 gccgcgtcat taaagccgtg ctgatgaacg ccgccgacaa aacggtcggc tgggacaacg
     1561 gtcaatcgct cgttggcggt gtcgtgcgca cgacgcaggc gctggactac gccaccggcg
     1621 cgggccgcgt cgatctgtcc aag
//
LOCUS       DATIMH010000741         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4218987, whole genome shotgun sequence.
ACCESSION   DATIMH010000741 DATIMH010000000
VERSION     DATIMH010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4218987"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VLI90_13010"
     CDS             complement(<1..416)
                     /locus_tag="VLI90_13010"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSV15172.1"
                     /translation="MKLLHIDSSVLGPHSVSRQVSAAIVDRLRKATPHLDVGYRDLTL
                     TPLAHLTGSHLAAGQGAVPEAALQPDIAAGQAVLEEFLAADIVVLGAPMYNFTIPSQL
                     KAWIDRILVAGKTFKYGAQGAEGLAGNKRVIIAISRG"
     gene            515..919
                     /locus_tag="VLI90_13015"
     CDS             515..919
                     /locus_tag="VLI90_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSV15173.1"
                     /translation="MKPEHTNVPVLPPSPHLDSDCRGVASILARVGDKWSVFVIMMLG
                     GGPKRFNEIKRLIGGISQRMLTLTLRGLERDGLVTRTVFPTIPPRVDYELTDLGRGLS
                     KPVEALGKWAFDHRVEIESARTKFDGRNDPAL"
     gene            970..1389
                     /locus_tag="VLI90_13020"
     CDS             970..1389
                     /locus_tag="VLI90_13020"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HSV15174.1"
                     /translation="MNADPWGDRVIKQKRQSAGVTMRARTGAILGLGAILITAPAWAA
                     GRYDPAYPVCMEAYGESGSRIECFFTTMEQCKQSASGSSGACFNNPGYVAPPPEPAAA
                     PIAEAAPVKPAKPKSTKSANAAKSLHPPPAQQPAPQR"
     gene            complement(1415..>1714)
                     /locus_tag="VLI90_13025"
     CDS             complement(1415..>1714)
                     /locus_tag="VLI90_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15175.1"
                     /translation="DLIKQGATLTTEAADVINAVTPIMERPVMLGAGEPDNEPLDFEA
                     GASERAVIIDLLGPSPVSLDDLIRCPALRPRSCAPCCSNSNSPDGSNVTAAGWSR"
BASE COUNT          330 a          528 c          548 g          308 t
ORIGIN      
        1 ccgcgcgaga tcgcgataat gacgcgcttg ttgcccgcca gtccttcggc gccttgcgcg
       61 ccatatttga acgttttgcc cgccaccagg atgcggtcga tccaggcctt gagctggctc
      121 ggaattgtaa agttgtacat cggcgcgccg agcacgacga tgtcggcggc cagaaactct
      181 tccagtacgg cttggccggc ggcgatgtcc ggctgcagcg cggcctcggg tacggcgccc
      241 tgaccggcgg cgagatgcga tcccgtcaga tgcgccagcg gcgtcagcgt caggtcgcga
      301 tagcctacgt cgaggtgagg ggttgccttg cgcaggcggt cgacgatcgc agcagaaacc
      361 tggcggctga cggagtgggg gcccagcacg ctggagtcga tatgcagaag cttcataggt
      421 tcaccctggt tggtataagt ttgtaactgg cgctatatga gtgaccacct atatctcccg
      481 caagaacgca cctttttgaa acccgagcac aaatttgaaa cccgagcaca ccaatgtgcc
      541 tgtcctgccg ccgagtccgc acctcgatag cgactgccgt ggcgtcgcct cgatcctggc
      601 gcgggtcggc gacaaatgga gcgtgttcgt catcatgatg ctgggtggcg gcccaaaacg
      661 cttcaacgag atcaagcgcc tgatcggcgg catctcgcag cggatgctga cgttgacatt
      721 gcgcggcctc gaacgcgacg gcttggtgac gcgaaccgta ttcccgacga ttccgccgcg
      781 cgtcgattac gaactcaccg atctcggccg cggtctgtcg aagccggtcg aggcgctcgg
      841 caaatgggcg ttcgatcacc gggtggagat cgaaagcgcg cggacgaagt ttgacgggcg
      901 caacgatccg gcgctttaga cctgtctcga tcattgccga acgtttcccg aagattgcca
      961 attcgtcacg tgaacgccga cccgtggggc gatagggtta tcaagcaaaa acgccaatca
     1021 gcgggggtga cgatgcgggc acggactggc gctattcttg ggctaggagc gatcctgatc
     1081 acggcaccgg catgggccgc cggtcgctat gatcccgcct atcccgtttg tatggaggcc
     1141 tacggagaaa gcggcagccg tatcgaatgc ttctttacaa cgatggagca gtgcaagcag
     1201 agcgccagcg gctcgtccgg agcgtgcttc aacaacccgg gttatgttgc accaccgccg
     1261 gagccggctg cggcgccaat agctgaagca gcgcctgtca aaccagcgaa gccgaaatcg
     1321 acgaagtcag ccaacgcggc gaaatcactg cacccgccgc cggcgcagca acccgcgccg
     1381 cagcgctgag cgcggaggca atcccggttc tagatcagcg agaccatccc gccgccgtga
     1441 cgttcgagcc gtccggcgag ttcgagttcg agcagcacgg tgcgcacgat cgtgggcgaa
     1501 gcgccggaca tcggatcagg tcgtcgaggc tgaccgggct gggcccaagc agatcgatga
     1561 taacagcgcg ttcacttgca ccggcttcga aatcgagcgg ctcattgtcc ggttcgccgg
     1621 cgcccaacat gaccggccgc tccatgatcg gcgtcaccgc gttgatgacg tcggcggctt
     1681 ccgtggtcag cgtcgcgccc tgcttgatca ggtc
//
LOCUS       DATIMH010000742         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1623705, whole genome shotgun sequence.
ACCESSION   DATIMH010000742 DATIMH010000000
VERSION     DATIMH010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623705"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..809
                     /locus_tag="VLI90_13030"
     CDS             87..809
                     /locus_tag="VLI90_13030"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HSV15176.1"
                     /translation="MATKTENAIRYPKVVDHAEWKKARDALLLQEKAATRARDALAAE
                     RRRLPMVKIDKPYTFTGPKGKLSLLDLFEGRRQLLLYHFMFAPPVPGWPDAGCVGCSM
                     VADQFGNLAHFHARDTSLCLVSVAPLKNIERFKKRMGWSYPWYSSEGTDFNKDFGLTT
                     DKGENYGLSVFIHDDDGNVYRTYFTDGRGVEALNIWTFLDLTPLGRQEEWEDSPLGGP
                     RTKPFVWWRRHDEYGNQSAGQH"
     gene            809..1123
                     /locus_tag="VLI90_13035"
     CDS             809..1123
                     /locus_tag="VLI90_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15177.1"
                     /translation="MGNCPFCNLDSAPVEQRRTSLARRAWRATQWFFPGAMLVLMPKC
                     PFCVAAYVALFTGVGITVSTARWIQILMIGFCLISLAYLLVRHNPFFSRSNDVNRTSD
                     AG"
     gene            complement(1206..1529)
                     /locus_tag="VLI90_13040"
     CDS             complement(1206..1529)
                     /locus_tag="VLI90_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15178.1"
                     /translation="MHAQLAAVVDPVVHHEHAQDHRFRNGDDRFAVEHAGPVGRQPGV
                     VQRGQDLSHRCAALFVRVENFLHRGRLLEQLGGVWQRRFIGQRETRHELRQRRDVQRQ
                     FAKRV"
     gene            complement(1788..>2920)
                     /locus_tag="VLI90_13045"
     CDS             complement(1788..>2920)
                     /locus_tag="VLI90_13045"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HSV15179.1"
                     /translation="MSDDDDFSFRDALPDGIAHRRGAGINPGNRFETVRLHVLGEELD
                     RQWIERQNEGGGPAQRVERQVFADRTQTIINHVAPTSDVPFDWTINPYRGCEHGCIYC
                     FARPYHEYLGFSCGLDFETKLVAKMDAPALLKRELASPRWRPEPIVMSATTDIYQPIE
                     HKLRIARECLQVLADCGQPVSTMTKSALVLRDTDLWAKLAQMNAGRVTVTLVTLDADL
                     AQRLEPRASSPAGRLRIIRELTEANIPVTVNVAPIIPGLTDVELPSILQAVADAGARR
                     ANWAMLRLPYQVKDLFLDWLKRAVHPDRARKVESLIRQTFGGKLYRANLDQRRRGRGV
                     HADQIAQTFDVFTRKYGLNRDVRPLSTAHFRRPQLHGQMALF"
BASE COUNT          548 a          977 c          858 g          537 t
ORIGIN      
        1 cttgtctttg ctcttcgtgc ccttcgtgcc cttcgtgccc ttcgtggttc aattccttcc
       61 cgaagaatcc gaccggaggc tcaatcatgg caacgaaaac cgaaaacgcg attcgatatc
      121 caaaagtcgt agatcatgcc gagtggaaga aggcgcgcga cgcgttgctg ctgcaggaaa
      181 aagccgccac gcgcgctcgt gatgccctcg ccgccgagcg ccggcggttg ccgatggtca
      241 agatcgacaa gccctacacc ttcaccggtc ccaaaggcaa actgagtttg ctcgatctct
      301 ttgaaggccg ccggcagctc ctgctgtatc acttcatgtt cgccccgccg gtgcccggct
      361 ggcccgacgc gggctgcgtg ggctgctcga tggtcgccga ccaattcggc aacctcgccc
      421 acttccacgc gcgcgacacg tcgctgtgtc tcgtctccgt cgcgccgctg aagaacatcg
      481 agcgcttcaa gaagcgcatg ggctggtcgt acccgtggta ctcctccgaa ggcaccgact
      541 tcaacaaaga cttcggcctc accaccgaca agggcgaaaa ctacggcctc agcgtcttca
      601 ttcacgacga cgacggcaac gtctaccgca cctatttcac cgacggccgc ggcgtcgagg
      661 cgttgaacat ctggacgttc ctggacctca cgccgcttgg ccggcaggaa gagtgggaag
      721 attcaccgct gggcggcccg cgcaccaagc cgttcgtctg gtggcggcgt cacgatgaat
      781 acggcaacca aagcgcaggg cagcactgat gggcaactgt ccgttttgca atctcgattc
      841 cgcgccggtt gagcagagac gcacgagcct tgcccgccgc gcctggcggg cgacgcagtg
      901 gttcttcccc ggcgcaatgc tggtgctgat gcccaagtgc ccgttctgcg tggccgcgta
      961 cgtcgcgctg ttcaccggag taggcatcac cgtctcaact gcgagatgga tccagatcct
     1021 gatgatcgga ttttgcctga tctcgttggc gtatcttctt gtccgacaca atccattttt
     1081 ttcgaggagc aatgatgtca atcgcacaag cgatgctggg tgagttcgaa cacgagtgcc
     1141 agaccacgcg caaattcctg gagcgcgtgc cggcggacat gctgcgctgg cggccgcacg
     1201 agaagtcaca cacgcttggc gaattggcgc tgcacatcgc gacgctgccg gagttcgtga
     1261 cgcgtttcgc gctggccgat gaatcgccgc tgccaaaccc cgccgagctg ttcaagcagc
     1321 cggccacggt gcaggaaatt ctcgacgcgc acgaaaagag cagcgcagcg gtgagacaga
     1381 tcctgcccac gctggacgac gcccggctgg cgaccaactg gtccggcatg ctcgacggca
     1441 aaccggtcgt caccattccg aaatcgatgg tcctgcgcgt gttcatgatg aaccactgga
     1501 tccaccaccg cggccagctg ggcgtgcacc tgcgcctcgc aggcgcgaag gtgccgtcga
     1561 gctacgggca agcggcgatg agggctgacc gcgcgtattc caaccacgcc gggttcggag
     1621 cgtactcgcg acgacccgga tcgaatggtg aaatgcagaa cgatgaatgc agaatgcagg
     1681 atggaatagg ctcgcgctgc cgttcatcgt tcatcattct gcattcaagg cttatccggg
     1741 tcgtcggagt acctccgaac ccggcgtggg ggttcagtaa atccgattca aaacaacgcc
     1801 atctgcccat gcagctgcgg ccggcgaaaa tgtgccgtcg acaacggccg cacatcgcga
     1861 ttcagcccgt acttcctcgt gaacacgtca aatgtctgcg cgatctgatc cgcgtgcacc
     1921 ccgcggccgc ggcggcgttg gtccaagttc gctcgataca acttcccgcc gaaggtttgg
     1981 cggatcagcg attccacctt gcgggcccga tccggatgca ccgcgcgctt caaccaatcg
     2041 agaaataaat ccttcacctg atacggcaag cgcagcatcg cccaattcgc ccgccgcgcg
     2101 ccggcgtcgg ccaccgcctg cagaatcgac ggcagctcga cgtccgtcag ccccggaatg
     2161 atcggcgcga cgttcaccgt caccggaatg ttcgcctcgg tgagttctcg aatgatccgc
     2221 aacctgccag ccggtgacga ggcccgcggc tccagccgct gcgccaggtc cgcgtccagt
     2281 gtcaccagcg tcaccgtcac ccggccggcg ttcatctgcg ctagcttcgc ccacaggtcc
     2341 gtgtcgcgca gcaccagcgc gctcttggtc atcgtgctga ccggctgccc gcagtcggcc
     2401 aggacttgca gacattcgcg cgcgatgcgc agcttgtgtt cgatcggctg atagatgtcg
     2461 gtcgtcgccg acatcacgat cggctccggc cgccagcgcg gcgacgccag ctcccgcttc
     2521 aacagcgccg gcgcatccat cttcgccacc agcttcgtct caaaatccaa cccgcagctg
     2581 aagccgagat actcgtgata cggccgagcg aagcagtaga tgcagccatg ctcgcacccg
     2641 cgatacggat tgatcgtcca gtcgaacggc acatcggacg tgggcgcgac gtggttgatg
     2701 atcgtctgcg tgcgatcggc gaacacctgc cgctcgaccc gctgcgccgg cccgccgcct
     2761 tcgttttggc gttcgatcca ttgccgatcg agctcttcgc ccagcacgtg caggcgaacc
     2821 gtctcaaacc gattgcccgg attgatcccc gccccgcggc gatgggcgat gccgtccggc
     2881 agcgcatcgc ggaaagaaaa gtcgtcgtcg tcgctcatgc
//
LOCUS       DATIMH010000743         1783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4507858, whole genome shotgun sequence.
ACCESSION   DATIMH010000743 DATIMH010000000
VERSION     DATIMH010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4507858"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VLI90_13050"
     CDS             <1..658
                     /locus_tag="VLI90_13050"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HSV15180.1"
                     /translation="NQTYATVTITAIDDMRVEGDETATFSVLANANYDLGTPNTATIT
                     IHDNDGAVLRDNADAYVRDGASANTNFGSATDIEVKKNMTGVNRQSYIKFDLTSVSAA
                     NIGSIQLRLYGSLNNANQQNVATGVFGSNNTTWSENAITWNNKPGTNTIALASATIIN
                     TTPQWYTWDITANAKSQLALGHKTITLVLQNSATSDPFATFASKESSSLGPQLVVAFA
                     "
     gene            complement(711..>1783)
                     /locus_tag="VLI90_13055"
     CDS             complement(711..>1783)
                     /locus_tag="VLI90_13055"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HSV15181.1"
                     /translation="AIDSLPDPVFVLNPAGRIELTNDAAGRWFEINPGDLPPEALRAW
                     VERALRQLAGADGAAIADASGYESTIQLNRDGSERHLLPRAVPIRDEQQRLIGATVVL
                     NDVTGLRRLDQMKSGLLALVSHELKTPLTSMRMILHLLADDRPNGLPTGDQLRELLIT
                     ARDDSDRLHQIVENLLDMGRIESGRVLMELQPIDAAELIGRSVRPLRPAYGEHGVELR
                     VEALPADVQVMADATRIGHVFSNILSNALRYTPAGGLVRIGARTLPEAVEIDLSDNGC
                     GVPRAHLHRVFEKFYRVPGQSRGGAGLGLAIVKDIIEAHGGRIAIESDQGRGTTVRFT
                     LNRAGRERHDEAAIASASVAVE"
BASE COUNT          345 a          629 c          534 g          275 t
ORIGIN      
        1 gaaccagacg tacgcgaccg tcaccatcac cgcgatcgac gatatgcgcg tggaaggcga
       61 cgagacggcg acgttctcgg tgctggccaa cgccaattat gacctgggca cgccgaacac
      121 ggcgacgatc accattcacg acaatgacgg cgcggtcttg cgcgacaacg ccgatgcgta
      181 cgttcgcgac ggcgcatcgg ccaacaccaa ctttggcagc gccaccgaca tcgaggtgaa
      241 gaagaacatg accggcgtga accgccagtc gtacatcaag ttcgacctga ccagcgtgtc
      301 ggccgccaac atcggcagca ttcagctgcg gctgtacggt tcgctgaaca atgccaacca
      361 gcagaacgtc gccaccggcg tcttcgggtc gaacaacacg acgtggtcgg agaacgcgat
      421 cacctggaac aacaagccgg gcaccaacac catcgcgctg gctagcgcca cgatcatcaa
      481 cacgacgccg cagtggtaca cgtgggacat caccgcgaac gccaagtcac agctggcgct
      541 gggccacaag acgatcacgc tggtgctgca gaacagcgcg acgagcgacc cattcgcaac
      601 gttcgcgtcg aaggaatcaa gcagcctcgg cccgcaactg gtggttgcat tcgcgtgagc
      661 aagcggcggt gcggcgagag caaacgccgt cccgcaaaaa ggcggagtcg tcactcgacg
      721 gcaaccgagg cgctcgctat cgccgcctcg tcgtgtcgct ctcgccccgc gcggttcaac
      781 gtgaatcgga cggtcgttcc gcggccctgg tcgctttcga tcgcgatccg cccgccgtgc
      841 gcttcgatga tgtctttcac gatcgccaaa cccaagcccg cgccgccgcg gctttggcct
      901 ggcacacgat aaaacttctc gaacacccga tgcaaatgcg cccgcggcac accgcagccg
      961 ttgtcgctca agtcgatctc gaccgcctcc ggtagcgttc gcgcgccgat gcgcaccaaa
     1021 ccaccggcgg gggtgtagcg cagcgcgttg gagaggatgt tcgaaaagac gtgaccgatg
     1081 cgcgtggcat ccgccatcac ctgcacatcg gccggcagcg cttcgacgcg cagctccacg
     1141 ccgtgctcac cgtacgcggg tcgcagcggt cgcacggaac gtccgatcaa ctccgccgcg
     1201 tcgatcggtt gcagctccat cagcacccgg cccgattcga tccggcccat gtccagcagg
     1261 ttctccacga tctgatgcag ccgatcgctg tcgtcgcgcg cggtgatgag cagctcgcgc
     1321 agctgatcgc ccgtgggcaa cccgttcggc cggtcgtccg ccagcaggtg caggatcatc
     1381 cgcatcgacg tcaacggcgt cttcagctcg tgcgatacca gcgccaacag accgctcttc
     1441 atctggtcca gcctgcgaag acccgtgaca tcgttcagca cgacggtggc cccgatcaac
     1501 cgctgctgct cgtcgcggat cggcaccgcc cggggcagga gatgccgctc gctgccgtcg
     1561 cggttcagtt gaatggtgga ctcgtacccg ctcgcgtccg caatggccgc gccgtcggca
     1621 cccgccaact gccgcagtgc ccgctcgacc cacgcccgca gcgcctcggg cggcagatcg
     1681 ccgggattga tctcgaacca tcgcccggcc gcatcattgg tcagctcgat ccggcccgcg
     1741 gggttcaaca cgaaaaccgg gtccggcagg ctgtcgatcg cca
//
LOCUS       DATIMH010000744         3443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1045515, whole genome shotgun sequence.
ACCESSION   DATIMH010000744 DATIMH010000000
VERSION     DATIMH010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045515"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1511
                     /gene="tig"
                     /locus_tag="VLI90_13060"
     CDS             132..1511
                     /gene="tig"
                     /locus_tag="VLI90_13060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HSV15182.1"
                     /translation="MAENEETGGTAVAEEKFEYPIKIEDAGPATKKVSVEIPADRIKS
                     KLDEQFKELRREAAIPGFRPGHAPAKLIAKRFGDDVKDQVRRSLISESYAQAVEKNDL
                     QVLGEPQFENEASISQLPDSGGLTYTFEIEVQPDITLPNLTGIQVKKPKIEVKDENVE
                     QAMNNLREQQGTLIPVEDRGVQDKDYITADVHIKLDGNVVAHQHDAQLVARPARIGGI
                     EVADFEKQIEGAKAGETKTITAKAPDNHPTEAIRGKDVQIEILVKDIKRLELVEITPE
                     FLEDLGFSSEQELRDALREQMVERIDYDVAQAQREQVMKYLLDNTTFELPRKMSAKQV
                     DRVVQRRAIDLLMRGVPREQVDAQLQHLRAGAVDEAARELKLFFILQKIANDQQTDVE
                     EAELNGRIALLAAQRGARPEKLKQEMAKDGSLSNLYVQMREQKALDKILESATIEEVD
                     VPNEPEKKE"
     gene            1694..2980
                     /gene="hisS"
                     /locus_tag="VLI90_13065"
     CDS             1694..2980
                     /gene="hisS"
                     /locus_tag="VLI90_13065"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSV15183.1"
                     /translation="MSTKIQAPKGTVDTLPAESWKWQAVERIAREVADLYDFREIRTP
                     IFEHSELFHRGVGETSDIVAKETYTFEDRGGRSITLRPEGTAGVVRAMIEHNTLADQG
                     ARTKVFYIGPNFRYENVQKGRVRQHHQFGVEAFGVAEPEQDVECILLQMDFYRRCGVK
                     DLELRVNSLGDRESKERYRDSLVAFLRPSAAQLSEDSKRRLDTNPLRILDSKDPRDQS
                     ACQSAPPAIESLSEKSRQHFERVQQSLASCRIPFRVDPTLVRGFDYYTETLWEVVAGG
                     LGAQNALGGGGRYDNLVEQLGGRPTPGVGFGSGLERLLIALESQGVELRDTRRPLVWI
                     VSQGESSKPVTWQLLSDLRQGGVAADMDLSGRGVKGQFKLADREGAKWCVIIGDSEVQ
                     HQTAVLKDMKTAEQTTLPRAELIARLRSSDCATAPA"
     gene            3110..>3443
                     /locus_tag="VLI90_13070"
     CDS             3110..>3443
                     /locus_tag="VLI90_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15184.1"
                     /translation="MTTRWLVASGLCATLLLAWRAAPPMWLRLRLLYWQHQCMQYTPS
                     SAQPVLQSDGKRQGFLRGVVPRPWSQFMNLLTQGSARTTATVFVHERTSPNGHRRLVG
                     VNLLAHVAP"
BASE COUNT          729 a         1076 c         1089 g          549 t
ORIGIN      
        1 gcatcgcgcc gctctttgca atccgccatt ccccgtatac gatgcccgac tttccccacc
       61 gggctgtgtg cccggcgtgg gtggttttgt cgacacacgc ttcgacacca acggataaga
      121 acgaggttgc catggccgag aatgaagaaa caggcggcac tgccgtcgcc gaggaaaagt
      181 tcgaataccc gatcaagatc gaggacgccg gcccggcgac caagaaggtg tcggtcgaga
      241 ttcccgcaga ccgcatcaag agcaagctgg acgagcagtt caaggaactg cgccgcgaag
      301 ccgccatccc gggcttccgt ccaggtcatg cgccggccaa gctcatcgcc aagcgcttcg
      361 gcgacgacgt gaaagaccag gtccgccggt cgctcatcag cgaaagctat gcgcaggcgg
      421 tggagaagaa cgacctgcag gtgctgggcg agccgcagtt cgagaacgaa gcatcgatca
      481 gccagctgcc cgattcgggc gggctgacgt acacgttcga gatcgaagtg caacccgata
      541 tcacgctgcc gaacctcacg ggcatccagg tcaagaagcc gaagatcgaa gtgaaggacg
      601 agaacgtcga gcaggcgatg aacaacctgc gcgagcagca aggcacgctg atcccggtcg
      661 aagaccgcgg cgtgcaggac aaggactaca tcaccgccga cgtgcacatc aagctggatg
      721 gcaacgtcgt cgcgcatcag cacgatgcgc aactggtcgc ccggccggcg cgcatcggcg
      781 gcatcgaagt cgccgacttc gagaagcaga tcgaaggcgc caaggccggt gagacgaaga
      841 ccatcaccgc caaggcgccg gacaatcacc cgaccgaagc gatccgcggg aaagacgtgc
      901 agatcgagat cctggtcaag gacatcaagc gcctggagct ggtcgagatc acgccggaat
      961 ttctggaaga ccttggcttc agcagcgagc aggaactgcg cgacgcgctg cgcgagcaga
     1021 tggtcgagcg catcgattac gacgtggccc aggcccagcg cgaacaggtc atgaagtacc
     1081 tgctggacaa cacgaccttt gagctgccgc gcaagatgtc ggccaagcag gtcgaccgcg
     1141 tggtgcagcg tcgggcgatc gacttgctga tgcgaggcgt gccgcgcgaa caggtcgacg
     1201 cgcagctgca acacctgcgc gccggcgccg tggacgaagc ggcccgcgag ctaaagctgt
     1261 tcttcatcct gcagaagatc gcgaacgatc agcagacgga cgtggaagag gcggagctga
     1321 acggccgcat cgccctgctg gccgcccagc gcggggcgcg gccggaaaag ctgaagcagg
     1381 agatggccaa agacggctca ctgtccaacc tgtacgtgca gatgcgcgag cagaaggcgc
     1441 tggataagat cctggagtcg gcgacgatcg aagaggttga cgtaccgaac gagccggaga
     1501 agaaagagta atcggccgtt ggtcatttcg aagctgaaag aagaggatta gccacagatg
     1561 cacgcagatg cacacagata agagaagcgg ccgagactca agtcccgtcg gcttctgcct
     1621 ttcatctgtg tcgatctgcg ttcatttgtt gctgagtcct ctttgttttt tattcgcgtg
     1681 attcgagctg acgatgagca cgaagattca agcgcctaag ggaacggtcg acacgctgcc
     1741 cgcggaatcg tggaagtggc aggcggtcga gcgcatcgcg cgcgaggtgg ccgatctgta
     1801 cgacttccgc gaaatccgga cacctatttt cgagcattcc gagctcttcc accgcggcgt
     1861 gggggagacc agcgacatcg tcgccaagga aacttacacg ttcgaagacc gcggcgggcg
     1921 gtcgatcacg ctgcgccccg aaggcaccgc cggcgtggtc cgggcgatga tcgagcacaa
     1981 cacgctggcc gaccagggcg cgcggacgaa ggtcttctac atcggcccga acttccgcta
     2041 cgaaaacgtg cagaagggcc gcgtccgcca gcaccaccag ttcggcgtcg aagccttcgg
     2101 cgtcgccgag ccagagcagg acgtcgagtg cattctgctg cagatggatt tctatcgccg
     2161 ctgcggggtc aaagatctcg agctgcgcgt caacagcctc ggcgatcgcg aaagcaaaga
     2221 gcgctatcgt gattcgctcg tcgcgttcct tcgtcccagc gcggcgcagc tgagcgaaga
     2281 ttccaagcgc cggctcgaca ccaacccgct gcgcatcctc gacagcaaag acccgcgcga
     2341 tcagtccgcg tgccaaagcg cgccgcctgc gatcgaatcg ctgtccgaaa aaagccgcca
     2401 gcatttcgaa cgtgtgcagc agtcgcttgc gtcttgtcgg atccccttcc gcgtcgatcc
     2461 cacgctggtc cgcgggttcg actactacac cgaaacgctg tgggaagtcg tcgccggcgg
     2521 actcggcgcg caaaacgccc tcggtggcgg cgggcgatac gacaacctcg tcgaacaact
     2581 gggcggccgg cccacgcccg gcgtcgggtt cggcagcggg ctcgaacgtc tgctgatcgc
     2641 gctggaatcg caaggcgtcg agctgcgcga cacgcgccgc ccgctcgtct ggatcgtgtc
     2701 gcaaggcgaa tcgtcgaagc ccgtgacgtg gcagctgcta tccgatttgc gccaaggcgg
     2761 cgtcgccgcc gacatggacc tcagcggccg cggcgtgaag gggcaattca agctcgctga
     2821 ccgcgaaggc gccaagtggt gcgtcatcat cggcgacagc gaagtgcagc atcaaacggc
     2881 cgtgctgaag gacatgaaga cggccgagca gacaacgctg ccgcgagcgg agctgatcgc
     2941 gaggctgcga tcgtccgatt gcgcgacagc ccctgcgtaa tccccctgga gccctgcatg
     3001 tacatgcggg gcttcattgg taaccatatc caccgtgtcg gacgcgccga tcaagcctcc
     3061 tgcaaatcca acgctgaact acgcgcgccc gccttcacat cgacgtcgct tgaccacccg
     3121 ctggctggtc gcgagtggcc tgtgcgcgac gttgctgctc gcctggcgcg cggcgccacc
     3181 aatgtggctg cggcttcgtc tgctctattg gcagcaccaa tgcatgcaat acacgccttc
     3241 ttccgctcag ccggtccttc aatcggacgg gaaacgtcaa ggcttcctgc gcggcgttgt
     3301 tccgcggccg tggtcgcagt tcatgaacct actcactcaa ggatcggcca ggacgacggc
     3361 gacggtcttc gtccacgaac gtaccagccc aaatggtcat cggcgcctgg tcggtgtgaa
     3421 tcttctagcg cacgtagctc cag
//
LOCUS       DATIMH010000745         1773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5380251, whole genome shotgun sequence.
ACCESSION   DATIMH010000745 DATIMH010000000
VERSION     DATIMH010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5380251"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VLI90_13075"
     CDS             complement(<1..674)
                     /locus_tag="VLI90_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSV15185.1"
                     /translation="MIRIANPFSGLESAVTEDAPLAPLTWYKIGGPTKYLVRPRSVEE
                     LREAARRCAENDIRVYVLGLGANILVSDQGVDGAVFRLDQEYWRRVKIDGLNLEVGAG
                     ADMQKLLLRSVRAGLAGIECLAGIPGTVGGGIRMNAGGKFGDIGAVVTRVQVMDANGT
                     TFERTKDDLVFEYRSTNIAATFILSATLELEQDDPDRIMRKTKEIWMYKRNTQPLNTK
                     NCGCIF"
     gene            complement(826..>1773)
                     /locus_tag="VLI90_13080"
     CDS             complement(826..>1773)
                     /locus_tag="VLI90_13080"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HSV15186.1"
                     /translation="QLGGGGSRSGASNAFVAEACEFDRSFHNLHPRVAIITNIEEDHL
                     DCYKDIDDIVESFRHFAAIVPADGLIIANGQDRRVLQALAGQKALIEMVALTDGFSWS
                     TRETGLSNGCYCGEVIYKGKAVAKLQLSVAGIHNLINATMAVAACASYGIAPAAAAEA
                     LSNFTGVDRRMTEVGRVNGGAIVVDDYGHHPTEIRATLKAIREKYQPRRLFCVFQPHQ
                     HSRTRFLLDDFATSFAEADETIVPDIYFVRDSEAERQRVSAADLVQRINDNGQRARHL
                     PDFAAIVAHLKEGARDGDLIVTMGAGNVWEIGRELTGLS"
BASE COUNT          307 a          564 c          544 g          358 t
ORIGIN      
        1 ttgaagatgc acccgcagtt cttggtgttc agcggctggg tgttccgctt gtacatccag
       61 atttccttgg tcttccgcat gatccggtcc ggatcgtcct gctccagttc cagcgtcgcg
      121 ctcaggatga aggtagcggc aatgttcgtg ctgcgatatt cgaaaacgag gtcgtccttc
      181 gtccgctcga aggtggtgcc gttggcgtcc atgacttgaa cgcgggtgac gaccgcgccg
      241 atgtcgccga actttccacc cgcgttcatg cggatgccgc cgcccacggt gccgggaatg
      301 ccggcgaggc attcaatgcc ggccaagccg gcgcgcacgc tgcggagcag caatttctgc
      361 atgtcggcgc ccgcgccgac ttcgagattc aatccgtcga tcttcacgcg acgccagtat
      421 tcctgatcga ggcggaagac cgcgccgtca acgccttggt cgctcacaag gatgttcgcg
      481 cccaggccca ggacataaac gcgaatgtcg ttctccgcgc atcgccgggc ggcttcccga
      541 agttcttcga ccgagcgggg ccggacgaga tacttggtcg ggccgccgat cttgtaccag
      601 gtgagcggcg ccagcggggc gtcttcagtg acggccgatt cgagaccgga aaatggattg
      661 gcgatgcgga tcatttctct tgtttatcct gagcgcaggc ggtgctccgc cggagtcgaa
      721 ggatctagcc gaggagtcag tgaactcgca ctctcgtcaa gttcgcagaa cgtagcttca
      781 cctactgccg gcgatatcct tcgactgcgg cgggtacgcc tgcgctcagg acaagccggt
      841 cagctcccga ccaatctccc acacattgcc cgcacccatc gttacgatca agtcgccgtc
      901 gcgcgctcct tcttttaaat gcgcgacgat cgccgcaaag tccggcaggt ggcgggcgcg
      961 ctggccgttg tcgttgatgc gctgcaccag atccgccgcg ctcacgcgct gccgttcggc
     1021 ttcgctgtcg cgcacgaaat aaatgtccgg cacgatcgtc tcgtccgcct cggcaaagct
     1081 ggtggcgaag tcatccagca agaatcgcgt tcggctgtgc tggtgtggtt gaaagacgca
     1141 gaacaggcgg cgcggttggt atttctcgcg aatcgccttc agcgtcgcgc gaatctccgt
     1201 gggatgatgg ccgtaatcgt caacgacgat cgcgccgccg ttcacccggc cgacctcggt
     1261 catccgccga tcgacaccgg tgaagttcga cagcgcctca gccgccgccg cgggggcgat
     1321 gccgtagctg gcgcacgccg ccaccgccat cgtcgcgttg atcagattat gtatgccggc
     1381 cacggaaagt tgcagcttcg cgaccgcttt gcctttgtaa attacctcac cgcagtagca
     1441 gccattgctc aggcccgtct cgcgcgtgga ccagctgaaa ccatcggtga gcgcgaccat
     1501 ctcgatcagc gccttctgcc cggccagcgc ttgcagcacg cggcgatcct ggccgttggc
     1561 gatgatcaga ccatccgcgg gaacgatggc cgcgaaatgg cgaaaggatt cgacgatgtc
     1621 gtcgatgtcc ttgtagcaat cgagatggtc ttcttcgatg ttggtgatga tcgcgacgcg
     1681 cgggtgcagg ttgtggaagc tgcgatcgaa ctcgcatgcc tcggcaacga acgcgttgct
     1741 cgcgcccgac cggctgccgc cgccacccag ctg
//
LOCUS       DATIMH010000746         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3928468, whole genome shotgun sequence.
ACCESSION   DATIMH010000746 DATIMH010000000
VERSION     DATIMH010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3928468"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VLI90_13085"
     CDS             complement(<1..789)
                     /locus_tag="VLI90_13085"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSV15187.1"
                     /translation="MPNELSRYESPLRWIDQQHERMCALVAAWASINSGTHNLDGLAR
                     LSAELRREFQVLGGETRELPVQPSTSVDARGQTVQMPLAPAISIRKRSSAALRVFLGI
                     HMDTVYGPEHPFQMVERDDEKSFRGPGVADAKGGLCVMLIALEALERSDFANRIGWEV
                     LINSDEEIGSPGSAPLLRECATRNKLGLVYEPAFADGALVGERKGSGNFTAVIRGRAA
                     HAGRDFHHGRNAIHAAAAMVMELDQINRRLPGVTVNVGRIDGGGP"
     gene            831..2210
                     /locus_tag="VLI90_13090"
     CDS             831..2210
                     /locus_tag="VLI90_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15188.1"
                     /translation="MSTTTTPNPLEQFRWTPQPAAEQLVKEIVDGIVARDAIAARFRD
                     RMRDEAGVRFIDCIDHLVVHESHVPLARLRDAGFERSDTLGGCYVHPGGIFPPIKISP
                     REAALVHLKVESVADFAAANGNVGAFVGQPGDRFRIGVVSRDFAVVERHGYRGPVTAF
                     AETPDHQVRLAMRHAELLRLRRRSFNNQIEGFAHAEELIDRSIHDLGVDLACDLFFAA
                     EREYWQRRNRAAQFQKARQDRLGFGWANHDHHTYRSSRKHFARLMSIFEKLGFRCRER
                     FYAGADAGWGAQVLEQPVTGIIIFADVDLSPDELFQDFAHEALSPREQLGTVGLWCAL
                     HGESFLQAGMHHLECQFDFESLKRQMEADGGIQVMKPFTDFPYLRQAFTEGERWPVAE
                     DRLQAVLVAGQITAEQAEKIRREGAIGSHLENLERNEGFKGFNQKGVSEIIAKTDPRK
                     HLSDTNSSA"
     gene            complement(2278..>2669)
                     /locus_tag="VLI90_13095"
     CDS             complement(2278..>2669)
                     /locus_tag="VLI90_13095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV15189.1"
                     /translation="CPLLTDRTMLSVVLRNLLINAVEYAPPGGRVRIETSNRGGDIEL
                     RIGNTNATLGDADLPHLFEPFWRKDPARTGGVHAGLGLALVATYCNALGIEIETSLTS
                     PDWFEVRLCIQRRAATSDERHELVSNA"
BASE COUNT          472 a          883 c          843 g          471 t
ORIGIN      
        1 cggccccccg ccgtcgatcc ggccgacgtt caccgtcacg cccggcaacc ggcggttgat
       61 ctggtccagc tccatcacca tcgccgccgc cgcgtgaatc gcgttgcgac catgatggaa
      121 gtcgcgaccg gcgtgagcgg cgcgaccacg gatgacggcc gtgaaattgc ccgagccttt
      181 gcgttcgccg acaagcgcgc cgtcggcgaa cgctggctcg tagaccaggc caagtttgtt
      241 gcgcgtcgcg cactcgcgca gcagcggcgc cgaaccgggc gatccgattt cttcatcact
      301 gttgatcagc acttcccaac cgattcgatt cgcaaaatcg cttcgctcca gcgcctccag
      361 cgcgatcaac atcacgcaca acccgccctt ggcgtcggca acgccggggc cgcggaagga
      421 cttctcgtca tcgcgctcga ccatctgaaa cggatgctcc ggcccataca ccgtgtccat
      481 gtggatgccg aggaagacgc gaagcgcggc tgacgatcgc ttgcggatcg agatcgccgg
      541 cgccagcggc atctgcaccg tttgcccgcg agcatcgacc gacgtgctcg gctgcaccgg
      601 cagctcgcgg gtctccccgc ccaacacctg gaactcgcgg cgaagttctg ccgataatcg
      661 cgctaggcca tcgagattgt gcgtcccgga attgatcgac gcccacgccg ccaccagcgc
      721 gcacatccgc tcgtgctgct gatcgatcca gcggagcggc gattcgtatc ggctcagttc
      781 gttaggcatg gaacggcgac ccgcgtacac ttaatctatc ggcacaaatc atgtccacca
      841 cgacaacccc caatccgctt gagcaattcc gctggacgcc gcagccggcg gcggagcaat
      901 tggtcaagga gatcgtagac ggcatcgtcg cccgcgacgc gatcgccgcc cgctttcgcg
      961 atcgcatgcg cgatgaagcc ggcgtgcgct tcatcgactg catcgatcat ctcgtggttc
     1021 atgaatcgca cgtgccgctg gctcgcctgc gcgacgcggg cttcgagcgc tccgacacgc
     1081 tcggcggttg ctacgtccac cccggtggca tcttcccccc gatcaagatc agcccgcgcg
     1141 aagcggcact ggtgcacctg aaggttgaaa gcgtcgccga cttcgccgcc gccaacggca
     1201 acgtcggcgc gttcgtcggt cagccgggcg accgttttcg catcggcgtc gtcagccggg
     1261 atttcgccgt cgtcgaacgc cacggttatc gcgggccagt gacggcgttc gccgagacgc
     1321 cggatcatca ggttcgcctg gcgatgcggc acgcggaact ccttcgcctt cgccgccggt
     1381 cgttcaacaa tcagatcgaa ggattcgcgc acgccgagga gttgatcgac cgctcgattc
     1441 acgatctggg cgttgacctg gcgtgcgacc tgttcttcgc cgccgagcgc gaatactggc
     1501 aacgccgcaa tcgcgcggct cagttccaga aggcccggca ggatcggctc ggcttcggct
     1561 gggcgaacca cgatcaccac acgtaccgtt catcgcgaaa gcatttcgcg cggctgatgt
     1621 cgatctttga aaagctcggc ttcagatgcc gcgagcgttt ctacgccggc gctgacgccg
     1681 gttggggcgc acaggttctc gaacagccgg tcaccggcat catcattttc gccgatgtcg
     1741 atctgtcgcc ggatgaactg ttccaggact tcgcgcacga agcgctgtcg ccgcgggagc
     1801 agctggggac ggtgggcctg tggtgcgcgc tgcatggcga atcgttcctg caggcgggca
     1861 tgcatcacct ggagtgccag ttcgatttcg aatcgctgaa gcggcagatg gaagccgatg
     1921 gcgggatcca ggtgatgaag ccgttcacgg attttccgta ccttcgccag gcattcaccg
     1981 aaggcgagcg ctggccggtg gcggaggatc ggctgcaggc ggtgctcgtt gccgggcaga
     2041 tcacggcgga gcaggcggag aagatccgcc gcgaaggcgc gattggctcg cacctggaaa
     2101 acctcgaacg caacgagggg ttcaaggggt tcaaccagaa gggcgtgagc gagatcatcg
     2161 cgaagacgga cccgcggaag catttgtcgg atacaaactc ttccgcgtag cggtagcacc
     2221 gcaggggcgc gtgttttcgt ttccagagga agaacgcgtg cccctgcggg gctaccgcta
     2281 cgcatttgag acgagctcgt gtcgttcatc gctcgttgcg gcgcgccgct ggatgcacaa
     2341 gcgcacctca aaccaatccg gcgacgtcag cgacgtctcg atttcaatcc ctagcgcgtt
     2401 gcagtacgtc gccaccagcg ccaaccccag tcccgcgtga acgccgccgg tgcgggcggg
     2461 atccttccgc cagaacggct cgaacaggtg cggcagatcg gcatcgccca gcgtcgcgtt
     2521 ggtgttgccg atgcggagct cgatgtcgcc gccgcgattc gacgtttcaa tgcgcacgcg
     2581 cccgccgggg ggcgcgtatt caaccgcgtt gatcagcagg ttgcgcagga cgaccgacaa
     2641 catggtccgg tcggtcagca gcgggcagt
//
LOCUS       DATIMH010000747         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1915518, whole genome shotgun sequence.
ACCESSION   DATIMH010000747 DATIMH010000000
VERSION     DATIMH010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915518"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VLI90_13100"
     CDS             complement(<1..935)
                     /locus_tag="VLI90_13100"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HSV15190.1"
                     /translation="MKILDRYVLTLFLKNYLISLAVLIGLYVIMDMVFNFDHLVSVQK
                     NTTTTGLTTALSVVRDVFSYYFYQSFMIFVQLSGIIPVVAAAFTLLRLSRFGELTAFL
                     AAGVPLLRVTVAIMCAAILLNALLIIDQEYVLPRMIPKLTRKHEEVHTAAVPYFPIRS
                     MQVDQRSLLVAARFWPATNDRPARLEEMDVIERDEMLRPVSHLFAKLAEWDSEHQDWQ
                     LTDGRIVTGLLPHQQPSDEQPVAVYRGDVTPEEIALYHSSEFVELLSTRRINELIARP
                     KSYGAVGLYRVKHMRFTQPIMNVVLLLLAIPTVLT"
     gene            1135..1389
                     /locus_tag="VLI90_13105"
     CDS             1135..1389
                     /locus_tag="VLI90_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15191.1"
                     /translation="MDRFKRLASGAVDAFDPVLYMTIAVFLRKVRRGAIRRPSHLSRK
                     LEPLVRRPIAAERADVQVKLAKPLINLQPLNSIEVVHASS"
     gene            complement(1407..>2961)
                     /locus_tag="VLI90_13110"
     CDS             complement(1407..>2961)
                     /locus_tag="VLI90_13110"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HSV15192.1"
                     /translation="GLDGSQILLLIRYSMPAMSTYSLPIAALFATTWVYGRMAADNEI
                     TACRASGISFLAMTTPAMFLGLFVAMMSMYLLCFTVPKNTLKIERVIVSNIAKIIVHQ
                     IEQTHETHFGYFTVYAESAHLVDPNPDKPNQQAVVLRGPMIVSFAAPPARDKFFNVAK
                     EFYMAREAVAYIDEDPGEGSDQMTVVLSDGVKLPRRFAAPKAEQGGLGVAQFGPVDIP
                     SRLGEKTKFMDIFQLKELDRDPSRGRDVQNVTAQFIAEDQAAEFTRRLSALFNSQERE
                     WNFDTGGDGYSIIRPPDAKVEIHGGMITMTAAPGGKPLIFRQQSGGQLRLTAESRSIE
                     ISTDADTDRDQLIVSVELRDVLVDAGETQTPKSELPRKFVLPMTADISAMKTRTPDIY
                     LGTAVRREEQRQRLLFAWVDLINHVRSEMHARAAFVVSCLLLVLVGASLGMMFRSGNF
                     LTAFAVSVVPAMLSVVLIVTGQHTAENVPKDLTAFNNPLNAGISIIWVGNVIIAVGAI
                     VLLTRLQRK"
BASE COUNT          554 a          919 c          905 g          583 t
ORIGIN      
        1 tgcgtcagca ccgtgggaat cgccaagagc agcagcacga cgttcatgat cggctgcgtg
       61 aatcgcatgt gcttcacgcg atagaggccg accgcgccgt agctcttagg gcgcgcgatc
      121 agctcgttga tccgccgggt cgagagcaac tcgacgaatt cgctgctgtg gtatagcgcg
      181 atttcttccg gcgtgacatc gccgcgatag acggcgaccg gttgctcgtc cgacggctgc
      241 tgatgcggca ggaggccggt gacgatccgg ccatccgtca actgccaatc ctgatgttcg
      301 ctatcccact cggcgagttt ggcgaacagg tgcgacaccg gtcgaagcat ctcgtcgcgc
      361 tcgatgacgt ccatctcttc cagccgggcc gggcgatcgt tcgtcgccgg ccagaaccgg
      421 gcggccacca gcagcgaacg ctggtccacc tgcatcgagc ggatcgggaa atacggcact
      481 gccgcggtgt gcacctcttc gtgcttgcgc gtgagcttcg ggatcatccg cggcagcacg
      541 tattcctgat cgatgatcaa cagcgcgttg agcaggatgg ccgcgcacat gatggcgacg
      601 gtcacgcgca gcagcggcac ccccgcggcg agaaacgcgg tgagttcacc aaaccggctc
      661 aatcgcagca gcgtaaacgc cgccgcgacg accgggatga tcccgctgag ctgcacgaag
      721 atcatgaacg attgatagaa gtaatagctg aagacatcgc gcacgacgct aagggccgtg
      781 gtcaggccgg ttgtggtcgt gttcttctgc accgagacga ggtggtcgaa gttgaacacc
      841 atgtccatga tgacgtacag cccgatcagc acggcgaggc tgatgaggta gttcttgagg
      901 aagagcgtga ggacgtagcg gtctagtatt ttcactggag tggtgagggg tcaggggtca
      961 ggggtcagga gtgaggtttg ggcgttgggg ccactttctc gactcgggag aaagttgccg
     1021 ttcgagagtt ctttctcggc cattgagcat gcggacgcac tcgtgatatc gcgcatgaag
     1081 ttcctcgtac ctttccaacg agatatagcc cttacgactg gcgatgtaca agtgatggac
     1141 cgtttcaagc gcctcgcctc tggcgcggtt gatgccttcg atcctgttct ttacatgacg
     1201 atcgctgtgt ttctccgcaa ggttcgccgg ggcgctattc gaagaccgtc tcacctgagc
     1261 cgcaagctcg aaccgctcgt gcgccggcca atcgcagctg agcgtgcaga tgtccaagtg
     1321 aagttggcaa agccactcat aaatttgcag cccctcaact cgatagaagt cgtgcatgct
     1381 tcctcctgac ccctgccccc tgacccctac ttccgctgca acctcgtcaa caacacgatc
     1441 gccccgacgg cgatgatcac attcccaacc cagatgatcg aaatccccgc gttcagcggg
     1501 ttgttgaacg ccgtcaggtc cttcggcacg ttttccgcgg tgtgctgacc cgtgacgatc
     1561 agcacgacgc tgagcatggc cggcaccacg ctgacggcga aggcggttaa aaaattcccg
     1621 ctgcggaaca tcatgcccag cgatgcgccg accaggacca gcagcaggca gctgacgacg
     1681 aacgcggccc gcgcgtgcat ttcgctgcgg acgtggttga tcagatcgac ccacgcgaac
     1741 agcagccgct ggcgctgctc ttcgcggcgc acggcggtgc cgaggtaaat gtccggcgtg
     1801 cgcgtcttca tcgccgagat atccgccgtc atcggcagga cgaatttccg aggcagctct
     1861 gacttgggtg tctgggtttc gccggcgtcc accagcacgt cccgcagttc gaccgagacg
     1921 atcagctgat cgcggtcggt gtcggcgtcg gtgctgatct cgatcgaccg cgactctgcc
     1981 gtcaggcgca gctgcccgcc gctctgctgg cggaagatga gcggcttgcc gccgggggcg
     2041 gcggtcatcg tgatcatgcc gccgtgtatt tcgaccttgg cgtcgggcgg gcggatgatg
     2101 gaatacccgt caccgccggt gtcaaaattc cattcgcgct cttggctgtt gaacagcgcg
     2161 ctcagtcgcc gcgtgaactc cgccgcctgg tcttcggcga tgaactgtgc ggtcacgttc
     2221 tgcacgtcgc gcccgcgaga cgggtcgcga tccagctcct tgagctggaa gatgtccatg
     2281 aacttcgtct tctcgcccag gcgcgacggg atatcaaccg gcccgaattg tgcgacgccc
     2341 agcccgcctt gttccgcctt aggcgcggcg aaccgccgcg gcaacttcac cccgtcgctc
     2401 agcaccaccg tcatctggtc cgatccttcg cccggatctt catcgatgta cgcgaccgct
     2461 tcccgcgcca tgtaaaattc cttggcgaca ttgaaaaatt tatcccgcgc cggcggggcg
     2521 gcgaagctca caatcatcgg cccgcgcagc acgacagcct gctgattcgg cttgtccgga
     2581 ttcggatcga ccaggtgggc cgactccgcg tacaccgtga aatatccgaa gtgtgtttcg
     2641 tgcgtctgct cgatctggtg cacgatgatc ttggcgatgt tcgacacgat gacccgctcg
     2701 atcttcagcg tgttcttcgg caccgtgaag cacagcaggt acatcgacat catcgcgacg
     2761 aacaatccca ggaacatcgc cggcgtggtc atcgccagga agctgatccc gctggcccgg
     2821 caggcggtga tctcattgtc agccgccatc cggccgtaga cccacgtcgt cgcgaacagc
     2881 gctgcgatgg gcagcgagta ggtactcatc gccggcattg aatagcggat gaggagcagg
     2941 atctgcgagc catccaagcc g
//
LOCUS       DATIMH010000748         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5666556, whole genome shotgun sequence.
ACCESSION   DATIMH010000748 DATIMH010000000
VERSION     DATIMH010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5666556"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..>2394
                     /locus_tag="VLI90_13115"
     CDS             89..>2394
                     /locus_tag="VLI90_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15193.1"
                     /translation="MTRGRKIASIVAGVIVVPLLIGLIAGIIIVRTDWFRNTVRQKII
                     TAVEDATGGKVDIASFAFDWTHLRAKVHDFTIHGLEPAGSPPLLHAALLQVDLKLTSP
                     FKNFVDIAYLLVDQPAANVIVYPDGRTNIPAPKIKPKSDNKTGVETIVDLAIGKFDLR
                     NGAFTFGDRKSELNASGGGFRAQLGYNAINPQYTGEIDISPLHVQSAKNPPLDIDVKL
                     PVTVEKDKVTLTNAQLTTPQSRVVISGAMDHLIAPRTSAHVNASLALDEARRAFGLNN
                     VLDTAHGPKAVDLDVTGSMDENGKLALKSGHATIGASQIEASGPPDNLQFRSTLALGE
                     LGRLLRVSARPEGELKIGGNAAIECGGNYRVTANLDARGVAIHQGTTRISGVNLDSAI
                     TADPHRIALDGLRLSALGGGFTGSAALEEMQRFHLSGSLHNFDIQQTLRSVAGTNIGY
                     DGVISGPVQANGDIKNMSTLQAKANLGIAPGRRGVPVSGHLGVDYDGRSGNVILDRSH
                     IALPHTAADLSGTLGRQIHVHVVSRDMADFRPLGAIPVTFSPGGAATVDANITGSLSD
                     PRVSAQAAVNNFAVEGRPFTRFDASLAASKSSAAVSNAVLSRGTLQAQFSGSVGLHNW
                     KPENYEPLRADAAVRNADLRDVLALAEQSSIPATGALTANAHFAGTVGSPTGNADLSI
                     LRGTIEGEPFDSLTAQVAATPTAINLPAFHFIAGPSHIDATAAYQHAMNDLQRGTVTA
                     HVASNQVQLAQFQTLVKDRPGLGGVLTL"
BASE COUNT          468 a          825 c          698 g          403 t
ORIGIN      
        1 gcgccacctc cgtcgaccag cagatggaag cctggctgaa gcacgcgcgc accaacagca
       61 agatcgtatt caaggcggag gctttccaat gacgcgcggc cgcaaaatcg catcgatcgt
      121 ggctggtgtc attgtagtgc cgcttctcat cggcctcatc gcgggcatca tcatcgtgcg
      181 caccgactgg ttccgcaaca cggtgcggca gaaaatcatc acggcggtgg aagacgccac
      241 cggcggcaaa gtcgatatcg catccttcgc tttcgactgg acgcatcttc gcgctaaagt
      301 ccacgacttc accattcacg gactggaacc ggctggttca ccgccgctgt tgcatgcggc
      361 gctgctacag gtggacctga agctgacctc gcccttcaag aacttcgtgg atatcgccta
      421 cctgctggtg gatcagccgg ccgccaacgt catcgtctat cccgatggcc gcaccaacat
      481 tcccgctccc aagattaagc caaagagcga caacaagacc ggcgtggaaa ccatcgtgga
      541 cctcgccatc ggtaaattcg atctgcgcaa cggcgcgttc acattcggcg atcgcaagag
      601 cgagctgaac gccagcggcg gcggcttccg cgcgcagctt gggtacaacg ccatcaatcc
      661 ccagtacacc ggcgagatcg atatctctcc gctgcacgtc cagtcggcca agaatccgcc
      721 gctggatatc gacgtgaaac tgccggtcac cgtggagaag gacaaggtga ccctcaccaa
      781 cgcgcagttg accacgccgc aatcgcgcgt cgtgatctcg ggcgcgatgg accacctgat
      841 cgcgccccgc acctcggcgc acgtgaatgc cagcctggcg ctcgacgagg ctcgccgcgc
      901 cttcggccta aacaacgtcc tggacacggc gcacggaccc aaagccgtgg atctcgacgt
      961 caccgggtcg atggatgaaa acggcaaact cgcactcaag agcggccacg ccaccatcgg
     1021 cgccagccag atcgaagcat ccgggccgcc ggataatctg cagttccgct ccacgcttgc
     1081 cttgggcgaa ctgggccgac tgctgcgcgt ttccgcgcgg cccgaaggag agctgaagat
     1141 cggcggcaat gcggcgatcg aatgcggcgg aaattaccgc gtcacagcca atctcgacgc
     1201 tcgcggcgtc gccattcatc aggggaccac acgaatttcg ggggtcaatc tggattccgc
     1261 catcacggcc gatccgcatc gcattgcgct agacggactc cgcttgagcg ctctcggcgg
     1321 cggtttcacg ggctccgcgg cgttggagga gatgcagcgc ttccatttgt ccggcagcct
     1381 gcacaatttc gatatccagc agacgctgcg atccgtggca ggcacgaaca tcggctacga
     1441 cggagtgatc tcgggaccgg tccaggcgaa cggcgacatc aagaatatgt cgaccctgca
     1501 ggctaaagcc aacctgggca tcgctcccgg aaggcgtggc gtgccggtct ccggacacct
     1561 cggcgtggac tacgacggcc gcagcggcaa cgtaattctc gatcgttcgc acatcgcgct
     1621 gccgcacacc gcggccgacc tgagcggcac gctcggacgc cagatccacg ttcatgtggt
     1681 ctcgcgcgac atggccgatt tccgtccgct cggcgccatt cccgtcacgt tttcacccgg
     1741 tggcgcggct accgtcgatg cgaacatcac cggcagcctc agcgatcctc gggtaagcgc
     1801 gcaagccgcc gtcaacaact tcgcggtgga aggccgcccc ttcacgcgct tcgatgcatc
     1861 cctcgccgcg tccaagtctt cggcagccgt cagcaacgcc gtgctctcgc gcggcactct
     1921 gcaggcgcaa ttcagcggca gcgtcggact tcataactgg aagcccgaga actacgagcc
     1981 cctgcgcgcc gacgccgccg ttcgcaatgc cgatctgcgc gatgtactgg cgctggccga
     2041 gcagtcttcg attcccgcaa cgggcgcgct caccgccaac gcgcatttcg ctgggaccgt
     2101 gggatcgccc accggcaacg ccgatctttc gatccttcgc ggcacgatcg aaggcgaacc
     2161 cttcgacagc ctgacagcgc aggtggcggc gactccgacc gccatcaatc tgccggcctt
     2221 ccacttcatc gccgggccct cgcacatcga tgcgacggcg gcctatcagc acgctatgaa
     2281 cgatctgcag cgcggcaccg tgactgcgca cgtggccagc aatcaggtgc aactcgcgca
     2341 attccagacg ctggtgaaag atcgtcccgg cctgggcggc gtcctcacgc tgaa
//
LOCUS       DATIMH010000749         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3928859, whole genome shotgun sequence.
ACCESSION   DATIMH010000749 DATIMH010000000
VERSION     DATIMH010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3928859"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VLI90_13120"
     CDS             <1..579
                     /locus_tag="VLI90_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV15194.1"
                     /translation="TLSLAANQTAIQSASSFPLNCSVLSIDGINFGSASQLDLTNNEL
                     VTTTPLATVRGWLTAGSIFSSSAGGVLGYASRGDGTVAVRFTLVGDVDLDGQVGVADL
                     GILATDYGTTTQGGWGAGDLNADGAIGVADLGMLATNYGQSLTGGSVATSQAMPSSTA
                     VPEPSAALVLVAGTLASSRRRGRRPAPNMAHS"
     gene            complement(680..1336)
                     /locus_tag="VLI90_13125"
     CDS             complement(680..1336)
                     /locus_tag="VLI90_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HSV15195.1"
                     /translation="MMRPRDAAEAARAGVDAIGLVFYPAAARRVSVDQAKQIIAALPP
                     FVTPVGLFVDAPPDEIRDVSKHLNLRHVQLHGDESPAVVEALPNVTVIKAIRVSRDGL
                     DQTLSTWRAVIHELKLNHLAALLMETARTAAPGGTGVENDWAAIAAAQQRGAFDGLPP
                     LIAAGGLTPENVGDVVRRLRPWAVDVSSGVESSRGVKSLEKMTSFMAAVREADRGRSR
                     "
     gene            1496..>2686
                     /locus_tag="VLI90_13130"
     CDS             1496..>2686
                     /locus_tag="VLI90_13130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HSV15196.1"
                     /translation="MANHFLAFDLGAESGRALLGTLDNGRLTLDEQHRFSNPNGRING
                     HWHWSLMGQWEHLKEGLRKAGKAGKEINGIGVDTWGVDFGLLSKDGDILGNPYMYRDA
                     RTDGVMDAVFARVDRRRVFEQTGTAFMQFNTLFQLYAMKHASPAVLDAADKLLFMPDL
                     FNYLFTGIKKNEFSITSTSQMYDMRQKRWATELMEILGLPTKILADVVPSGTVIGALR
                     ADVAEECGVPPMNVIAPAGHDTGSAVAAVPAAGGDSWCYISSGTWSLMGLELPEPIVN
                     DKSLKYNYTNEGGIGGTTRFLKNIMGLWLVQECRRHWVKEGHDHSYAELTQMAERATT
                     TALIDPDYQPFLSPGNMVPKIDAFCQQKKQRCPSSRGEYVRCCLESLALTYRRTLEGL
                     DDILG"
BASE COUNT          482 a          917 c          821 g          466 t
ORIGIN      
        1 acattgagcc tcgccgcgaa ccagaccgca attcagtcgg cgtcgtcgtt cccgctgaac
       61 tgcagcgtgc tgtcgatcga tgggatcaac ttcggctcgg cgtcgcagtt ggacctgacc
      121 aacaacgaac tggtgaccac caccccgctc gccacggtgc gcggctggtt gaccgcgggg
      181 tcgatcttct cgtccagcgc gggcggcgtg ctcggctacg catcgcgcgg cgacggcacc
      241 gtggcggtgc gcttcacgct cgtgggcgac gtcgatctcg acggccaagt cggtgtggcc
      301 gacctcggca tcctggcgac cgattacggc acgactacac aaggcggctg gggcgcgggt
      361 gatctcaacg cagatggcgc catcggcgtg gctgacctgg gcatgctggc gaccaactac
      421 ggccaatctc ttaccggcgg cagcgttgcg acatcgcaag cgatgccatc gtccaccgcc
      481 gtgccggaac cgtcggcggc gctggtgctg gtggccggta ccctcgcctc gtcccgacgg
      541 cgcggccgcc gccccgcgcc gaatatggct cattcgtagc acgggcgtct cgcccgtgcc
      601 tgacgactcg gcacgggcgg cacgcccgtg ctacgaattt tcgcgatgtc gtcttgcttg
      661 gcgggcgcgc accccggggt caccggctcc gtccgcgatc ggcttcccga acggccgcca
      721 tgaagcttgt catcttctcg agcgacttta cgccgcgcga tgattcgacg ccgctgctca
      781 catccacggc ccatggccgc aggcgacgca cgacgtcccc aacgttctcc ggtgtgagtc
      841 caccggcggc gatcagtggg ggtagaccgt caaacgcccc gcgctgctgc gccgcggcga
      901 tggcggccca gtcgttctcg acgcccgtcc cgcctggagc ggcggtgcgc gcggtctcca
      961 tcaacaacgc ggcgagatgg ttcagtttca gttcgtgaat caccgcgcgc caggtcgaca
     1021 gcgtttgatc gagcccatcg cgactcacgc ggatcgcctt gatcacggtc acgttcggca
     1081 gcgcctcgac gaccgcgggc gattcatcgc cgtgcaactg cacgtgccgc aggttcaggt
     1141 gcttgctcac gtcgcggatc tcgtccggcg gcgcgtccac gaacagaccg accggcgtca
     1201 caaacggcgg cagggccgca atgatctgct tcgcctggtc caccgaaacc cgtcgagccg
     1261 cggcggggta gaaaaccaaa ccgatcgcgt ccacccccgc gcgcgccgcc tccgcggcgt
     1321 cccgcggtcg catcatccca cagaacttga tccgcgtgcg gtgcatcctt ttccaggaga
     1381 gcgggcccgt ccccgggccg taattccggg tcgaggacag cccggctccc cttcgaatgc
     1441 tacgtcaccc atcccgcgtt taccatttcg cgtttctcgc taacatccat cgcttatggc
     1501 caaccatttc ctcgcgtttg atctcggcgc tgaaagcggc cgcgcgctgc tgggtacctt
     1561 ggacaacggc cgactcacgc tcgacgagca gcatcgattc tccaatccca acggccgcat
     1621 aaacggccac tggcattgga gcttgatggg ccaatgggaa catttgaaag aaggcctgcg
     1681 caaagcgggc aaggccggca aagaaatcaa cggcatcggc gtggacacct ggggtgtgga
     1741 cttcggcctg ctcagcaagg acggcgacat cctcggcaat ccttacatgt atcgcgacgc
     1801 ccgaaccgat ggcgtgatgg acgccgtctt cgcgcgcgtc gatcgccgcc gcgtcttcga
     1861 gcagaccggc accgcattca tgcagttcaa cacgctgttc cagctttacg cgatgaagca
     1921 cgccagcccc gccgtgctcg acgcggctga caagctgttg ttcatgccgg acctgttcaa
     1981 ctacctgttc acgggcatca agaagaacga gttctccatc acctcgacca gccagatgta
     2041 cgacatgcgg caaaagcgct gggcgacaga gctgatggag atacttggat tgccgacgaa
     2101 gatccttgcc gacgtggtgc ccagcggcac ggtgatcgga gcacttcgcg ccgacgtcgc
     2161 cgaagagtgc ggcgtgccgc cgatgaacgt catcgccccc gcgggccacg acaccggtag
     2221 cgccgtcgcc gccgttcccg ccgcgggcgg tgattcctgg tgctacatca gctccggcac
     2281 ctggtcgctg atgggcctgg aattgcccga gcccatcgtc aacgacaaat ctcttaaata
     2341 caactacaca aacgaaggcg gcatcggcgg gaccacgcgg ttcctgaaaa acatcatggg
     2401 cctgtggctc gtgcaggaat gccgccggca ttgggtgaag gaaggccacg accacagcta
     2461 cgcggaactg acccagatgg ccgagcgcgc caccacgacc gcgctgatcg atcccgatta
     2521 tcagccgttc ctctcgcccg gcaatatggt gccgaagatc gacgcgtttt gtcagcagaa
     2581 aaagcagcgc tgccccagca gccgcgggga atatgtacgc tgctgcctgg aaagcctcgc
     2641 gctgacttat cgaagaacgc tcgaaggttt agacgatatc ctcggc
//
LOCUS       DATIMH010000750         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3354043, whole genome shotgun sequence.
ACCESSION   DATIMH010000750 DATIMH010000000
VERSION     DATIMH010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3354043"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..2997
                     /locus_tag="VLI90_13135"
     CDS             259..2997
                     /locus_tag="VLI90_13135"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HSV15197.1"
                     /translation="MASHHLAPFPEELLAAKKQIREQARAYGLDFYPVIFEMCDYEQM
                     NQIAAYGGFPQRYPHWRFGMEYERLRKQHHYGLGRIYEMVINNDPCYAYLQESNALVD
                     QKLVIAHVYGHADFFKNNLWFSQTNRKMMDEMANHGTHVRKHIEKHGQDVVERWLDIC
                     LSVEHLIDPHSMFLKRGPVDEVPQQPKREDPVEKFQAKDYLDRWINPPKALEAEAKRR
                     REEKAKMRHATPARPTRDVLLYLLEHARLEDWQADCLSIIREESYYYAPQGMTKVMNE
                     GWACVLPESLIFTDGGVIVMRELVESRAKPMVSDGERRQRVYDHNIIAERDVVKVRTR
                     RGLCMGGSNNHRIMMPNGEWRQLDELKPGDRVRIAGGGDLWASERVSIDWHPQQRVGL
                     DDVARESGVSVWTVLRHRAGKRVREAAAVSTALAVYDAPANLALPQAMSKRRVCRIPR
                     VVDERLAAFLGYLIGDGHVSRVERNVGLTTADDEQIQSFVALSDDLFGLECAVKPDGG
                     RTRVLLHSQMLSDFLVDGIGLTHGPSARRKRVPQAILRSPKPVVAAFLRAYFDCDGYA
                     GKQGVILSTSSDSLAEVVQLLLLNFGILSSRRPHKDGCWHVHVAGKSCEAFEREINFG
                     LARKRAALRKYLADHVWFKEESWDDEIVAIEHDRADVYDISVEETHRYAAQGFINHNS
                     FWHSTLMTKHFVEATEIIDYADHHSGTVHMPPGNFNPYKIGIELFRDIEDRWNKGKFG
                     KEYEETETLGVKKRWDKGLGQGREKIFEVRRVYNDVNFIDEFMTPEFIEKHKFYQYGR
                     DPHTGQLRIVSRDPQRIKQTMLYQLTNMGQPFIYVVDGNYCNRGELYMAHRHNGLDIE
                     IKFAVETLKNIYKIWQRPVHLQARIDDDMILFSYAGDQPKQQTIHDDLPKPAHQVG"
BASE COUNT          666 a          921 c         1000 g          594 t
ORIGIN      
        1 tgcaggtttc ccgtcttcgc atgcgctgcc tttcacgaat cgcgtcgcgg tgatctcggc
       61 ccgcgtggaa aactcgcgaa cctcggccat ctgtgccggc gtcagcgggt gatcgatggc
      121 aagaaactcg taatactgat attcgctcat gcgtcacgct cacgttggct ggcgctcgaa
      181 tgtatgcgcg acccaagccg cgcgaggata gaatgtagtc gcgacgctcc acgcggttca
      241 attgccgatt ggattcttat ggcttcacac cacctcgccc cgttccccga agaattgctc
      301 gcggccaaaa agcagatccg cgagcaggcc cgcgcctacg ggctcgattt ttatcccgtc
      361 attttcgaga tgtgcgatta cgagcagatg aaccagatcg ccgcgtacgg cggcttcccg
      421 cagcgctatc cgcactggcg cttcggcatg gaatacgagc gcctgcgcaa gcagcatcac
      481 tacgggcttg gccgcatcta tgagatggtc atcaacaacg acccctgcta cgcctacttg
      541 caggagtcga acgcgctcgt cgatcagaag ctggtcatcg cgcacgtgta cggccacgcc
      601 gacttcttca agaacaacct gtggttcagc cagaccaacc gcaagatgat ggatgagatg
      661 gccaaccacg gcacgcacgt gcgcaagcac atcgaaaagc acgggcaaga tgtggtggag
      721 cgctggctcg acatctgcct gagcgtcgaa cacctgatag acccgcacag catgttcctg
      781 aagcgcggcc cggtggatga agtgccgcaa cagccgaagc gcgaggaccc ggtcgagaag
      841 tttcaggcga aggattatct cgatcgctgg attaatccac ccaaggccct ggaagccgaa
      901 gcgaagcggc ggcgcgaaga aaaggcgaag atgcgccacg cgacgccggc ccggccgacg
      961 cgcgacgtgc tgctgtatct gctcgaacac gcgcggctcg aagactggca ggcggactgc
     1021 ctctcgatca tccgcgagga gagctactac tacgcgccgc aggggatgac gaaggtaatg
     1081 aacgagggct gggcctgcgt ccttcccgag agcctgattt tcactgacgg cggcgtcatc
     1141 gtgatgcgcg agttggtgga gtcgcgcgcg aagccgatgg tttccgacgg cgagcggcgt
     1201 cagcgcgtct atgaccacaa catcatcgcc gagcgcgacg tggtgaaggt gcgcacgcgt
     1261 cgcggactgt gcatgggcgg atcgaacaac caccgcatca tgatgccaaa tggcgaatgg
     1321 cggcagctcg atgagctaaa acccggtgat cgcgttcgta tcgcaggtgg cggcgatctt
     1381 tgggccagcg agcgcgtgtc gatcgattgg catccgcagc agcgcgttgg tttggacgat
     1441 gtcgcgcgcg aatccggcgt gtcagtctgg acggttctcc gacatcgcgc cggcaagcgc
     1501 gtccgcgaag ccgccgcggt atcaacggcg ctggcggtat acgatgcgcc ggcgaacctg
     1561 gcgctcccgc aggcgatgtc gaagcgccgc gtctgccgaa tccctcgcgt cgtggacgag
     1621 cggttggcgg cgttcctcgg ttacctgatc ggcgacgggc atgtctcgcg cgtggagcgg
     1681 aacgtcgggc tgacgacggc ggacgatgag cagatccaat cgttcgtggc gctgtcggac
     1741 gatttgtttg gcctggagtg cgcggtgaaa ccggacggcg gccgcacgcg ggtgctgctg
     1801 cattcgcaga tgctgtccga cttcctcgtt gacggcattg gattgacgca tgggccaagc
     1861 gcccggcgga aacgcgtgcc gcaggcgatt ctccgctcgc caaagccggt ggtcgcggcg
     1921 ttccttcgcg catacttcga ttgcgacggc tacgccggga agcagggcgt catcctttcc
     1981 acgtcgagcg attcgctggc ggaagtcgtg cagttgctgt tactgaactt cggcatcttg
     2041 tcgagccgtc ggccgcacaa ggatgggtgc tggcacgtgc acgtcgccgg caaatcgtgc
     2101 gaagcgttcg aacgcgaaat caacttcggt ttggcccgca agcgcgccgc gctgcgcaaa
     2161 tatctggcgg atcacgtgtg gttcaaggaa gagtcctggg atgatgagat cgtcgcgatc
     2221 gagcacgatc gggccgacgt ctacgacatt tcggtcgaag aaacgcatcg ctacgctgcc
     2281 cagggcttca tcaatcacaa cagcttttgg cattcaacgc tgatgaccaa gcacttcgtc
     2341 gaagcgacgg agatcatcga ctacgccgac catcacagcg gcacggtgca catgccgccg
     2401 gggaatttta atccgtacaa gatcggcatc gagttgttcc gcgacatcga agaccgctgg
     2461 aacaagggaa agttcggcaa ggaatacgaa gagacggaaa cgctcggcgt gaagaagcgt
     2521 tgggataagg ggctgggcca gggccgggag aagattttcg aagtccgccg ggtgtacaac
     2581 gacgtgaact tcatcgacga attcatgaca ccggaattca tcgagaagca caagttttat
     2641 cagtacggcc gcgacccgca cacggggcag ctgcgcatcg tcagccgcga tccgcagcgc
     2701 atcaagcaga cgatgctgta ccagctgacc aacatgggcc agccgtttat ctacgtggtg
     2761 gacggcaatt attgcaaccg tggcgagctg tacatggccc atcgccacaa cgggctggac
     2821 atcgaaatca agttcgcggt ggaaacgctg aagaacattt acaagatctg gcagcgcccg
     2881 gtgcacctgc aggcgcggat cgatgatgac atgattttat tttcctacgc gggcgatcag
     2941 ccgaagcagc agacgataca cgatgatctg ccgaagccgg cgcatcaggt gggatgaggg
     3001 atgcgcagac gctttgatgt caaaggcgct tctccagccg ctggctgtct aacgtacctt
     3061 gttttttcga taagcataat tggtttctcg gttgccgcga ccaacataac ctttcggctc
     3121 actgagcgcg ccgtcttgaa gaagctatac ggcgtcgatg cgtacgatca cgggctcagg
     3181 g
//
LOCUS       DATIMH010000751         9558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1625319, whole genome shotgun sequence.
ACCESSION   DATIMH010000751 DATIMH010000000
VERSION     DATIMH010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625319"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1844
                     /locus_tag="VLI90_13140"
     CDS             <1..1844
                     /locus_tag="VLI90_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HSV15198.1"
                     /translation="AIDQVASMPIKDGFRGAVELSPGSFTCGGALNITTSGIVLRGSG
                     SGSGDGGTTIHMVGPKHRAIVIGAERGRPREERLPADDDPAPGVEPAASAPAAEFTAA
                     TTKIADAYVPEGTNSFTVASAKGFEVGDTIAIHRPTTAAWVHFMGMDTLKRDGKKQTW
                     IGLNRGGVTDRTITATDGNRITIDLPLADSYDAKFLNPPGTTVTKMKPAARVTRVGVE
                     HLHLQCPPLETSYGRAPYSAIRVGGDDCFVRDVYCEELMSDTVLAGRRITMQNVVCTH
                     TYPNMGASKPTDFSIEGSQILIDRCRITGGNMYFVWSASLYPGPNVILNSTFRGQGSR
                     IQPHMRWSTGLLVDNCTVPDGGIDFMNRGVAGSGHGWTMGWAVAWNCIAKTYVIQNPP
                     GAVNWAIGCIGERVQTARMFDTSPILPDGVFDSHGTPVTPQSLYLAQLSERLGPQALQ
                     AIGYSANDASAFPHKTTPPLPEQRAVVDPELGPNLALHRPVNATSAPESLPAISGDRA
                     VDGDDRTYWSPSMDAKHMTLEIDTEGPLDINAISLGEARGFESHVQEYKVEGQVNSDW
                     QLLAQGTTIGDRVVTRFPETTVWKVRLTILKTQGAPGIREFGLYLAK"
     gene            1932..2306
                     /locus_tag="VLI90_13145"
     CDS             1932..2306
                     /locus_tag="VLI90_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSV15199.1"
                     /translation="MRLSHVNITMPPGNEPRARAFYGDLLGLREIPKPAELLKRGGVW
                     FDAGGLDLHVSVEDDRAAAAVDHRRHFGLECADVDAMRDRMIAAGVEIDAGRPAPWRR
                     FFVRDPFGNRIEIHEPGALRGG"
     gene            complement(2390..3655)
                     /locus_tag="VLI90_13150"
     CDS             complement(2390..3655)
                     /locus_tag="VLI90_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HSV15200.1"
                     /translation="MARLLVIYLAVVSLIATVHAANVPATPGTEPSDGKDINMPANNS
                     FAADLYTRLAAGSPAGRGNLFFSPYSIRTALVMTYAGARGRTAEQMAGVLHLPAGPAD
                     QLNKSFASLIQQMNAKHDDYQLAVANALWGQSGYGFRDDFKAILRDDYGAALQEVDFA
                     HHAEQVRGQINQWAERATHDKIKDLVPPGVLTPATRLVLANAIYFKGAWQWPFHTSAT
                     RDEPFHLDASHDAKVPMMHHGQQTWGYTETPQLQAVELPYQGRDVSMVLVVPKAVDGL
                     GEIEKWISGQTLAQWQSSLRPQLVVLTMPKFTMTSAFRLAEVLGAMGMKDAFDASRAD
                     FSAMVTPQQQKTEPPYISEVIHKAFVAADEAGTEAAAATAVMVAAGAARMEMPKPVAV
                     IADRPFLFYIYHRPSGTVLFLGRVSDPRG"
     gene            complement(3701..3946)
                     /locus_tag="VLI90_13155"
     CDS             complement(3701..3946)
                     /locus_tag="VLI90_13155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HSV15201.1"
                     /translation="MFHPNFIEDLSFFVSNDRKRALKLLEIVRAVLREPFTGIGKPEP
                     LKALGADVWSRRLTDEHRVVYLVAHDRVHFLQARYHY"
     gene            complement(3983..4261)
                     /locus_tag="VLI90_13160"
     CDS             complement(3983..4261)
                     /locus_tag="VLI90_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15202.1"
                     /translation="MAASKSTFTRSNLSDVIDQALDENGAVIIKRPGRKDLLILPADR
                     FREFDTASYLLASSRNRTRLLAALRDAGAGKGRPMTVKSLRAKVGLTG"
     gene            complement(4337..4657)
                     /locus_tag="VLI90_13165"
     CDS             complement(4337..4657)
                     /locus_tag="VLI90_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15203.1"
                     /translation="MSVEKTPPPNNWWVQCRVCEVRDGRKALKWRIVIFIAPATDGQP
                     ADQAHFLSMNFTAANAQEADQLADRLAAEQGHRRITHRRSETLSCVWRWSDYWQPEFT
                     PAPI"
     gene            4979..5053
                     /locus_tag="VLI90_13170"
     tRNA            4979..5053
                     /locus_tag="VLI90_13170"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5014..5016,aa:Pro,seq:tgg)
     gene            5380..5544
                     /locus_tag="VLI90_13175"
     CDS             5380..5544
                     /locus_tag="VLI90_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15204.1"
                     /translation="MLLPQVGKLALQIIGAAARFGLVVPRQLTQPLPDPVLHRLRLLD
                     AVEDGQHLRV"
     gene            5776..6720
                     /locus_tag="VLI90_13180"
     CDS             5776..6720
                     /locus_tag="VLI90_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15205.1"
                     /translation="MRADEPLAAIAHLADVGSVAQDVADLAARPGGHRVGVVDRHLRQ
                     LGGDHVDRAMVVGVEVEDQPHVLGFAVIDGDDAAAVDPLIAIAVGRHADEQSAGGAAG
                     QSFHHIDRLLLRVEAGHAGQHAPLHPARRRVLRRLADRDQRDVERAFNPLKLDVIEQV
                     SGSPVDLVEQHPVERLGMVLRVGDHLLERGAFVGLAGRLGDAIQLDDGAAGAFGVTLE
                     RVHLHVQAEAFTLLLAAGNAGQRNESLLLHGVGICSVTCDLLHENAPVAGAQGGLRSS
                     RKSRDFWWFVRGFRRKVRSTAFYFNRACLLNGQASHAR"
     gene            complement(6674..7039)
                     /locus_tag="VLI90_13185"
     CDS             complement(6674..7039)
                     /locus_tag="VLI90_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15206.1"
                     /translation="MTKADQRNELLCSRARRAGCEIYSGEAGKKTVLVLMPGFPEYGT
                     GVDMTAKIDAFLATGSPHVSQIIDAAVCQAEAENYYPAWNYVCDTYTPKEVARRESKA
                     LRRKIIARGWLVRSKDRHD"
     gene            complement(7014..7208)
                     /locus_tag="VLI90_13190"
     CDS             complement(7014..7208)
                     /locus_tag="VLI90_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15207.1"
                     /translation="MIADARAVWEPIYGRALSADEINEIIFNVDGLLRVLFNLKGAVG
                     SSSHCHRQSGEDHDQGRSAE"
     gene            complement(7512..8771)
                     /locus_tag="VLI90_13195"
     CDS             complement(7512..8771)
                     /locus_tag="VLI90_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HSV15208.1"
                     /translation="MASAPRKPRVWATTRAEVRLLGRGAKLMAKKSAARLATPAVKKS
                     MVRLRELLRAERLCKLQQGDVLVQLIDRHQLRAIDIARRTIQRPSDLSQMYHTCRMFP
                     PRVRRRDVPYNTYFLATRIVRKFRHLKLSPGQVLAEIQRLGFSQHRQVTAHFAAKETA
                     ASAATVAATHLEFGAGPFDRAYHTPFQSLLKLFPDRLIKVLHVDPPYVYPRRAAGRYG
                     ASSARPLSCDNRDAVYAIALVNDLLREWQAKLAPGGVLLLWQPSGPLPHEISQAMDEF
                     HWEVDRVVIWDKGRPQAGDLAGAYQTQCEWLWVIKRPGERLLNHDASPRGDIVRFAPV
                     SAPQLAAEQQHAFEKPLDLCRFLVGKHSHPGELVFDCCGCTGSMSVAAIETGRRWVYA
                     ESNTQNHARGSARIARALGPQRTAQAG"
     gene            complement(9100..9375)
                     /locus_tag="VLI90_13200"
     CDS             complement(9100..9375)
                     /locus_tag="VLI90_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15209.1"
                     /translation="MSSEAAVARFRATADSWAEKYNKQTLSSAGISDEDKNRYNDAMS
                     KETELLKSGLIGDGETAEAATRMAKEEAIAAENRRHFRAEDAINGKR"
BASE COUNT         1730 a         2967 c         2986 g         1875 t
ORIGIN      
        1 cggcgatcga tcaagtcgcg tcgatgccga tcaaggatgg atttcgcggc gcggtcgagc
       61 tttcgccggg gtcgttcacc tgcggcggtg cgctgaacat caccaccagc ggaatcgtgc
      121 ttcgcggcag cggcagcggc agcggtgatg gcggaacgac gattcacatg gtcgggccga
      181 agcatcgcgc gatcgtgatc ggcgccgagc gcggccggcc gcgcgaagag cggctgcccg
      241 ccgatgatga tcccgcgccc ggtgtcgagc cggctgcgag cgcaccagcg gccgaattca
      301 ccgcggcgac cacgaagatc gccgatgcct atgtgccgga agggaccaac agtttcactg
      361 tcgccagcgc caagggtttt gaagttggcg acacgatcgc catccaccgc cccaccaccg
      421 cggcttgggt gcacttcatg ggaatggaca cgctcaagcg cgacgggaaa aagcagacat
      481 ggatcggcct caatcgcggc ggcgtgaccg atcgcaccat taccgcgacc gacggcaatc
      541 gcatcacgat cgatctgccg ctggcggatt cgtatgacgc gaagttcctc aatccgccgg
      601 gcacgacggt cacgaagatg aagccggccg cgcgggtcac gcgcgtgggc gtcgagcatc
      661 tgcacctgca atgtccgccg ttggagacct cgtatggccg cgcgccgtat tcggcaattc
      721 gcgtcggtgg cgacgactgc ttcgtgcgcg acgtgtattg cgaagagctg atgagcgaca
      781 cggtgctggc cggccggcgg atcacgatgc agaatgtcgt ctgcacgcac acgtatccga
      841 acatgggcgc gtcgaagccg acggacttca gcatcgaagg cagccagatc ctgatcgacc
      901 gctgccggat caccggcggg aacatgtatt tcgtctggtc ggccagtttg tatcccgggc
      961 cgaacgtgat tttgaacagc acgttccgcg ggcaaggcag ccggatccag ccgcacatgc
     1021 gatggtccac cgggctgctg gtcgataact gcacggtgcc cgatggcggg atcgatttta
     1081 tgaaccgcgg cgtggccggg tcgggtcacg gctggacgat gggctgggcc gtcgcgtgga
     1141 actgcatcgc gaagacgtac gtgattcaaa acccgccggg tgcggtgaac tgggcgatcg
     1201 gctgcatcgg tgagcgcgtg cagacggccc gcatgttcga cacgagtccg attttgccgg
     1261 acggcgtttt cgattcgcac ggcacaccgg tgacgccgca gagcttatat cttgcgcagt
     1321 tgagcgagcg cctcggcccg caggcgctgc aagcgatcgg ctacagcgcc aacgacgcca
     1381 gcgcgtttcc ccataagacc accccaccgc tgccggagca gcgcgcggtg gtcgatcccg
     1441 agctgggacc aaacctggcg ctgcatcgtc cggtgaacgc gaccagcgcg cccgagagcc
     1501 tgcccgcgat cagcggcgat cgcgctgtcg atggcgatga ccgcacgtac tggtcgccat
     1561 cgatggacgc gaagcacatg acgctggaga tcgacaccga gggtccactg gacatcaacg
     1621 cgatcagcct cggcgaagcc cgcggcttcg aatcgcacgt gcaggagtac aaggtggagg
     1681 gccaggtgaa cagcgactgg caactgctcg cgcagggcac gacgatcggc gaccgcgtcg
     1741 tcacgcgctt tcccgagacg acggtgtgga aagttcggct gacgattctc aagacgcaag
     1801 gcgcgccggg gatccgcgag ttcggcttgt atctcgcgaa ataatcagga tgcccccacg
     1861 ccgggttcgg aggtactccg acgacccgga tgcctattcg gatcaccacc agataacatc
     1921 gacatcaaac catgcgtctc tctcacgtca acatcacaat gccccccggc aacgagccac
     1981 gcgcccgcgc gttttacggc gacctgctcg gcctgcgcga gattcccaaa ccagcggagc
     2041 tgctcaagcg aggcggcgtc tggttcgacg cgggtggttt ggatctacat gtctccgtcg
     2101 aagacgatcg cgcggccgcg gcggtcgatc atcgacgtca tttcggcctg gaatgcgcag
     2161 atgtcgatgc gatgcgcgat cgaatgatcg ccgcaggcgt tgagatcgac gccggacggc
     2221 ccgcgccgtg gcggcgattc ttcgtgcgcg atccgtttgg taacagaatc gagattcacg
     2281 agccgggcgc gctgcgcggc ggataaagga cgcgtgcgcg atcgcctttg agatcgatca
     2341 ggatcgcgcc acatcggaat gccatgggcg gcgcttgtgc gattatcgct cacccgcgcg
     2401 ggtcgctcac gcggcccagg aacagcacgg tgccgctggg gcgatgataa atgtagaaca
     2461 ggaacggccg atcggcgatc accgcgaccg gcttgggcat ctccatccgc gcggcgccgg
     2521 cggcaaccat caccgctgtc gcggcggcgg cttcggtgcc ggcttcgtcg gcggcgacga
     2581 acgccttgtg aatcacctcg ctgatgtacg gcggctcggt cttctgctgc tgcggtgtca
     2641 ccatcgccga aaaatcggcg cggcttgcat cgaaggcgtc cttcatgccc atggcgccca
     2701 atacttctgc gagccggaac gcagatgtca tggtgaactt cggcatcgtc agcacgacca
     2761 gctgcgggcg cagcgacgac tgccactgag cgagcgtttg accgctgatc cacttctcga
     2821 tctcgcccaa tccatcaacc gccttcggca cgaccagcac catcgacaca tcgcggcctt
     2881 ggtacggcaa ctccaccgcc tgcagctgcg gggtttcggt gtagccccac gtctgctggc
     2941 cgtggtgcat catcggcacc ttcgcatcgt gcgatgcgtc gagatggaac ggttcatcgc
     3001 gcgtcgcgct ggtgtggaac ggccactgcc aggcgccctt gaagtagatc gcgttggcga
     3061 gcaccagccg ggtcgccggt gtcagcacgc cgggcggcac caggtctttg atcttgtcgt
     3121 gcgtcgcgcg ctcggcccat tggttgatct gcccgcgcac ctgctcggcg tggtgcgcga
     3181 aatcgacctc ctgcaatgca gcgccgtagt cgtcgcgcaa gatcgctttg aagtcatcgc
     3241 ggaatccata gccgctttgg ccccacaacg cgttggcgac cgcgagctgg taatcatcgt
     3301 gcttggcgtt catctgttgg atgagcgacg cgaagctttt gttcagctga tccgccggcc
     3361 cggccggcag atgcaggacg ccggccatct gctcggcggt ccgcccgcgc gccccggcgt
     3421 acgtcatcac caacgccgtg cgaatgctgt acggcgagaa gaacagattc ccccgccccg
     3481 ccggcgatcc ggcggccaga cgcgtgtaca agtccgcggc aaagctgttg ttggcgggca
     3541 tgttgatgtc ctttccatcg gaaggttcag tgccaggcgt tgccggcaca ttcgcggcat
     3601 ggaccgtcgc aatcaaggac acaactgcca ggtaaatgac gaggaggcga gccatgggac
     3661 ctcgctgggt tttgttcgcg aacaagaatg tcggcgacca tcaataatga tatcgcgcct
     3721 gcagaaaatg aacgcgatcg tgcgccacca gatagacaac tcgatgttca tcggtgaggc
     3781 ggcgtgacca cacgtcagca ccaagcgcct tgagcggttc cggctttccg atgccggtaa
     3841 acggttcacg caggaccgct cgcacgatct caagcagttt caaagcgcgc ttgcgatcgt
     3901 tgcttacgaa aaacgaaagg tcctcgatga agttcggatg aaacactgcg tcgcgtgcgc
     3961 gctagctgac catgatcgtg cctcacccgg tgaggccgac tttggcgcga agcgatttaa
     4021 ccgtcatagg gcggcctttg ccggcgcccg cgtcgcgaag agctgccaag agccgcgttc
     4081 gattcctgga tgaggcaagg agataactgg ccgtatcgaa ctcgcggaag cggtcagcgg
     4141 gcaggatcaa caaatctttt cggccaggac gcttgatgat gacagcgcca ttctcgtcaa
     4201 gcgcctggtc gatcacatca ctcaggtttg accgggtgaa ggtgcttttt gaggcggcca
     4261 tggagttacc ttatcgacgc cgcgatcaga tggtcaaggg cggcagccaa cggcactaca
     4321 gccatgcggg cacatctcag atcggcgccg gggtaaattc cggttgccaa taatccgacc
     4381 agcgccacac gcagctgagc gtttcgctgc ggcggtgggt gatgcgcctg tgcccctgct
     4441 cggccgccag ccggtcggcc agctggtccg cctcttgcgc gttggcggcg gtgaaattca
     4501 tgctgaggaa gtgcgcttga tccgccggct gaccgtcggt cgccggcgcg atgaagatca
     4561 cgatccgcca cttcagcgcc ttacgcccgt cgcgcacttc gcacacccgg cactgcaccc
     4621 accagttgtt cggcggcggc gtcttctcga cggacatcgg caaactctcc acgcgcgtga
     4681 ccgcggcgag tataccgttg ggacgcgcag gactgccaca tcacgcatat ggagcgcaga
     4741 tcctggtacg agtgcattag catcaggagg caaaggaaga gaaatggctg atttgcagca
     4801 cgggcgtctc gctgcgtctc gctgcgtctc gcccgtgccg aggttgtgcc gaggtgctag
     4861 acacgggcga gacgcccgtg ctacggtgac atcgaatcct cacgccgttg agccgacttg
     4921 cctttgcccg tcgagcgggg agaatgttga ctaagccgtt cgcgaacgcg agaaagaacg
     4981 gggcgtagct cagcttggct agagcgcctg gtttgggtcc aggaggtcgc aagttcgaat
     5041 cttgtcgccc cgattagctg ggatttttag cccttcgagc agccgctcga agggctgtct
     5101 catttctggc actagactta cgccatcgag ccgcaagttc aataacagaa agtcgagcag
     5161 cgcgcgcttt tctgcgtaat ccgccgcaag ccatttgtct gcaaggtgct gtgaaagttc
     5221 aaaacaacgc agcgcaaagt ccgctcgctc gtctcggtct cggtcggtcg cttccagctt
     5281 cacggtcagt ttcgccacgc ggtcgcgcag ctcggtgctc ttgctgcgga aggtgctctc
     5341 ttcgatctcg tcgttcaggc gcaggttcag caacaggtct tgttgcttcc gcaggtcggc
     5401 aagctggcgt tgcagatcat cggtgcggcc gcgcgattcg gtctggtcgt cccgcgtcag
     5461 ttgacgcagc cgcttcccga tccagtcctg caccgcctgc ggctgttgga tgcggttgaa
     5521 gatggccagc atctgcgcgt ctaagtcggc ttccttcagc cgcacacgcg gatggctgcc
     5581 cttgttgtac atcgtgcagc ggtagtagtg gtattgcttg ccgtttggct tggtgacggt
     5641 ctcgccggtg atcaagttgc cgcagtgacc gcagcgaatg agcgagccgg cgtagaccag
     5701 ttggtgggat ttgtaaacgt gcccgccaag taaatgctgc acgcggtccc acaccggtcg
     5761 ttcgatgatc ggctggtgcg agccgacgag ccactggccg cgatagcgca tctcgccgat
     5821 gtaggatcgg tcgcgcagga tgtggccgat cttgctgcgc gtccaggcgg gcaccgcgtt
     5881 ggtgtagttg atcgacatct ccgccagctt ggcggcgatc atgtcgatcg tgcaatggtg
     5941 gtgggcgtag aggtcgaaga tcagccgcac gttctgggct tcgcggtcat cgacggcgac
     6001 gatgctgcgg ccgttgaccc gctcattgcg atagccgtag ggcgtcatgc cgacgaacaa
     6061 tccgctggcg gcgcggcggg ccaatccttc catcacatcg accgactgct gctccgtgta
     6121 gaagctggcc atgctggcca gcatgcgccg ctgcatccgg cccgccggcg tgttctccgt
     6181 cggctggctg accgcgatca gcgggacgtt gaacgcgctt tcaatccgct caagctcgac
     6241 gtaatcgaac aggtttctgg cagcccggtc gatcttgtag aacagcatcc cgtcgagcgt
     6301 ctcggcatgg tccttcgcgt aggcgatcat ctccttgaac gtggtgcgtt cgtcggtctt
     6361 gctggccgtc tcggcgatgc gatacagctt gacgatggtg ccgccggcgc gttcggcgta
     6421 acgcttgagc gcgtccacct gcacgtccag gctgaagcct tcacgctctt gctcgcggct
     6481 ggaaacgcgg gccaacgcaa cgaatcgctt ctgcttcacg gcgtcggaat ctgctctgtt
     6541 acgtgcgatc ttcttcatga aaatgctcct gttgcaggag cacaaggagg gcttcggtcg
     6601 agccggaagt caagggattt ctggtggttt gttcgcggat ttcggcggaa agtccgttcg
     6661 actgcgttct attttaatcg tgcctgtctt ttgaacggac aagccagcca cgcgcgatga
     6721 tcttgcggcg aagcgccttg ctttcacggc gggcaacttc cttcggcgtg taggtatcac
     6781 agacgtaatt ccatgcgggg tagtagttct ctgcttcggc ctgacagacc gctgcgtcga
     6841 tgatctgcga cacatgcgga ctgccggttg cgagaaaagc gtcgatcttg gccgtcatat
     6901 cgacgccggt tccgtactca gggaagcccg gcatgagcac caacacggtt ttcttgcctg
     6961 cctcgccgct gtaaatctcg caacctgcgc gacgggcacg cgagcacaac aattcattcc
     7021 gctgatcggc cttggtcatg gtcttctcct gattggcggt ggcaatgact ggacgacccg
     7081 accgcgccct tcaggttgaa gagaacacga agcaggccat ccacgttaaa aatgatctcg
     7141 ttgatttcgt ctgcgctgag cgcccggcca tagatgggtt cccagacggc gcgggcatcg
     7201 gcgatcacgt cggcggggta gccgatgggt cgtacagagt ttggtggcgg ggacagcaca
     7261 tgactctatt tactcattta atccgcactt ccagaaatca cgctgcgaga agtttggcta
     7321 gccaaacttt ccctcgtgtc gcgcaagtgg cggggacgac gagggtgatc taatcgtgcc
     7381 tgacaaatgt cgggcgacgc ggtgacggcc gtggcggcga gcgatatgta ttcgtgccgg
     7441 ttgaatgaac ggagcgtgcg accccgctgc cagccggtca ggtaatcgtg cctgttagat
     7501 gagcggcagc gttaaccggc ttgggcggtg cgttgtgggc cgagagcgcg ggcgatccga
     7561 gcgctgccgc gggcgtggtt ctgggtgttg gattcggcgt atacccaccg ccggcccgtc
     7621 tcgatggccg ccacgctcat cgagccggtg cagccgcagc agtcgaagac cagctcacct
     7681 ggatgactgt gcttgccgac caggaaccgg cacaggtcca gcggcttctc gaacgcgtgc
     7741 tgctgctccg cggcgagctg cggtgcgctg accggggcaa agcgaacgat gtcgccgcgg
     7801 ggactggcat catgattcag cagccgctcg cccggccgct ttattaccca cagccattca
     7861 cactgagtct gatacgcccc ggctaagtcg cccgcctgcg gccggccctt gtcccagatg
     7921 accactcgat ccacttccca atggaattcg tccatcgcct ggctgatctc gtgtggtagt
     7981 ggcccggaag gttgccaaag cagcagcacg ccgccgggcg cgagcttggc ctgccactcc
     8041 cgcaacagat cgttcaccag cgcaatggca tagacagcat cgcggttgtc acagctcagc
     8101 ggccgagcag acgatgcgcc ataccggccc gccgcccgcc gcggatacac atacggcggg
     8161 tccacgtgaa ggaccttgat caaacggtcg ggaaagagct tcagcagcga ctgaaatggc
     8221 gtgtggtatg ctcggtcgaa cggaccagca ccgaattcca gatgcgtggc agcaacagtc
     8281 gccgcactcg ccgccgtctc cttggccgcg aagtgcgccg tcacctgccg atgctggctg
     8341 aagccaagac gttgaatctc cgccagcacc tgtcccggcg acagcttcag gtgcctgaac
     8401 ttccgcacaa tccgcgtcgc caggaagtag gtgttgtacg gcacatcccg ccgccgaacg
     8461 cgcggcggga acatccggca ggtgtggtac atctgcgaca gatcgctcgg ccgctgtatt
     8521 gtccttcggg cgatgtcgat cgcgcgaagc tgatgccggt cgatgagctg aaccagcacg
     8581 tcaccctgct gaagcttgca aagccgctcc gcccggagca gctcgcgcag ccgcaccatg
     8641 cttttcttca ccgccggcgt cgccagccgc gccgcggact tcttcgccat cagcttcgcg
     8701 ccgcgcccga gcaggcggac ctccgcccgt gtcgtagccc aaacacgcgg ctttcggggt
     8761 gcacttgcca tcgtgcggcc atgatagcgt gcccccttgg gcgcaccagc aacgacacga
     8821 gggcctattt cacctcggtg atgagcagca cccccacccc gggtttgatc ctttgcagtt
     8881 ttctgtgctt cgctgctgtc tctcgcctgg atcaatactt cgcggttcgt gctctcgctg
     8941 ttctttggga gcgttacctt ttaggtcttc acctcgtttg ccgccgctga ccatatggaa
     9001 cagcagccgc tgcggcggtg gccgcccacg gtgttttgaa tcgcaatggc gagcgaagcg
     9061 atgatatgat tcggagcatc gcacctccat ttacgcacgc taacgctttc cgttaattgc
     9121 atcttccgcc cggaagtgcc ggcggttctc cgcggcaatg gcttcttctt tggccatgcg
     9181 cgtcgccgct tcggctgtct cgccgtctcc gattaagcca gatttcagca gctccgtctc
     9241 cttgctcata gcatcattgt aacgattctt gtcctcgtcg ctgattccag ctgatgacaa
     9301 cgtctgcttg ttatactttt ccgcccaact atcggccgtc gctctgaaac gcgcaacggc
     9361 cgcttctgag gacatattcg tgtagctgcc gttcgattca aagagaagct ccgccggcgc
     9421 cgaatcccgc cgagaacagc ccggaggtac cgtccagatg atgatactgc tgacgagaag
     9481 cgccacttga gacactcgac aattcattgg tattatcctc gatctcggct gcatttggcg
     9541 ggacaaaatg cgtcatac
//
LOCUS       DATIMH010000752         4092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_178488, whole genome shotgun sequence.
ACCESSION   DATIMH010000752 DATIMH010000000
VERSION     DATIMH010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178488"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VLI90_13205"
     CDS             complement(<1..199)
                     /locus_tag="VLI90_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15210.1"
                     /translation="MFFETLENRSLFSVSLSAGVLTVTGTPAADVISIVKNASGGITV
                     TDDGVVDNFSSVSVAHVAVNAG"
     gene            complement(305..1750)
                     /locus_tag="VLI90_13210"
     CDS             complement(305..1750)
                     /locus_tag="VLI90_13210"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSV15211.1"
                     /translation="MSKLSFDIDSVIAGKARLNDAQALELYYSTSMHDLGQWAHAVTQ
                     RLHPEDYRTYVIDRNINYTNVCTAKCTFCAFRRDHEDADSYTLGYEKIGEKIRELVAI
                     GGTQILMQGGMNDALPIEWYEDLLRYIKHNFPTVHIHAFSPPEFIEFERFFQMDVRKI
                     IRRLHKAGLDTIPGGGGEIFTPRVRRRIGIGKCTGDDWLRVMRVAHEEGMNTSCTMLI
                     GHIEFVRERIEHMSALRDMQDYARALSVVRGQLSVAEEGCAAQSMREILARRPDVFRQ
                     SQRTTDDGQLTGSYTAFIHWPFQRENTPLGRAQEWDPAIHGPFDDSTNEDILRGRVVR
                     MAGADEYLRTLAIARLYFDNIPSLQASWVTMGPKIGQLAMFFGANDMGSVMMEENVVS
                     AAGTTYKLEEREICRLIRDAGWVPAQRDQYYNVLARHDGADSPDLRPVAHPPVRSVRK
                     LDKEYIGVAPGLDDGADRSVSLQLPILGGPR"
     gene            1908..3521
                     /locus_tag="VLI90_13215"
     CDS             1908..3521
                     /locus_tag="VLI90_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15212.1"
                     /translation="MKSYLLILVCAFSIGAVLKRTTPTPPTPDADATGVSKLVHLGSN
                     GKLVYEPYTEHGDVIPDFSNCGYMGGGVRIPDVAAKITLRPETGSGDDTARIQQAIDQ
                     LGNMPIGPDGFRGALVLSRGYYHIGGSLKIHASGIVLRGEGSGEGGTVLTATGNKPRA
                     MIVVASDAKQVIVESKQSPDVTDEYVPVGARSLGAVDASQFHVGDCIIVRRIGNAAWI
                     HELGMDDMPIQRSGTRDWKSFDIESDRVITAVNGNQITFDAPICCAIEKRWGGGEVVK
                     YTDAERIGQIGVENLRAISEFDKSVEKKLGDTSYYADEQHATDAISFNTCKNAWARNI
                     VAMRFVHGVAKMERGSKWITVQDSEAREPVSIITGGRRYPFNIMGQLCLVLRCRSDEE
                     RHAFVMNGSHDAGPDAFVDCTSEHNHADSGPHQRWSTGTLWDNVVGVMHTQDREDMGS
                     GHGWAGANDVYWNCSGRITIEQPPTAQNFAFGFVGQKDKAAFTQLHHPEGWYESFGKR
                     VQPPSLYMQQLKDRLGPRADENITPPGSH"
     gene            complement(3518..>4092)
                     /locus_tag="VLI90_13220"
     CDS             complement(3518..>4092)
                     /locus_tag="VLI90_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15213.1"
                     /translation="VSSSARAPAGVQPLLFPDSFELSELPLGWLGVIAAWIVPTTAPA
                     APAIAPAIDSAMGAARSLISTARSATPLATWFTFFVARFTIPFDLALALFALRVALFG
                     AAFIRPFMSFALDITRDAAFLAAFFIVLPVDFFVVFFADFFADFFPDFFADFFAAFLA
                     MQPPRGVVVRRCLYPNHRILPEKCRNSRQF"
BASE COUNT          828 a         1237 c         1314 g          713 t
ORIGIN      
        1 cgccggcgtt gacggcgacg tgcgcaacgg acaccgagct gaaattatcc acgacgccgt
       61 catcagtgac ggtgattccg ccactcgcgt tcttcacgat gctgatgacg tcggcggccg
      121 gtgtgccggt cacggtcaaa acgccggcgc tgagggaaac gctaaacagg ctgcgatttt
      181 caagggtttc gaagaacatc gggttacctc gttaaagatc cgcgtgtctg catgcggaga
      241 ggtaccccga atcaattcta attctgcggc aggattccat tcgggcaaaa tcgcgacaaa
      301 tggcctagcg tggccctccc aagatcggca gttgcaggct cacgctgcgg tccgccccgt
      361 catccagccc gggtgcgaca ccaatgtact ccttgtcgag cttgcgcacg ctgcgcaccg
      421 gcggatgcgc aaccggccga aggtcgggcg agtcagcgcc gtcgtgccgc gcgagcacgt
      481 tgtagtattg atcgcgctgc gccggcaccc accccgcgtc gcggatcaac cggcagatct
      541 cgcgctcttc cagcttgtac gtcgtgcccg cggcactgac gacgttctcc tccatcatga
      601 ccgagcccat gtcgttggcg ccgaagaaca tcgccagctg gccgatcttc gggcccatgg
      661 tgacccagga tgcttgaagc gacgggatgt tgtcgaagta cagccgcgcg atcgcaagcg
      721 tgcgaagata ttcatcggcg cccgccatgc ggaccacgcg cccgcgaagg atatcttcgt
      781 tggtgctgtc gtcgaacggg ccgtggattg ctggatccca ttcctgcgct cggccgagcg
      841 gcgtgttctc acgctggaac ggccagtgga tgaaggcggt gtaggatccg gtcagttgtc
      901 cgtcgtccgt cgtccgttgt gattgtcgga acacatcggg gcggcgggca aggatttccc
      961 gcatcgattg cgctgcgcac ccctcctcag ccacggacaa ctgaccacgg acgactgaca
     1021 atgcacgagc gtaatcctgc atatcccgca gcgcgctcat atgctcgatg cgttcgcgca
     1081 cgaactcgat gtgcccgatc agcatcgtgc agctggtgtt catcccctct tcatgtgcga
     1141 cgcgcatcac gcgcagccaa tcatcgcccg tgcatttacc gatgccgatg cgccgccgca
     1201 cgcgcggcgt gaagatttcc ccgccgccgc cggggatcgt gtcgaggccc gccttgtgca
     1261 accggcggat gatcttgcgc acgtccatct ggaagaaccg ctcgaactcg atgaactccg
     1321 gcgggctgaa ggcgtggatg tgaaccgtcg gaaaattgtg cttgatgtag cgcagcaggt
     1381 cttcgtacca ctcgatcggc agcgcatcgt tcatcccgcc ctgcatgagg atctgcgtgc
     1441 caccgatcgc caccagctcg cggatcttct cgccgatttt ttcgtaaccc agcgtatagc
     1501 tgtcggcgtc ttcatgatcg cggcgaaacg cgcagaacgt gcacttggcc gtgcagacgt
     1561 tcgtatagtt gatgttgcgg tcgatgacgt aggtgcggta gtcctccgga tgcagccgct
     1621 gcgtgaccgc gtgagcccat tggcccaggt cgtgcatcga cgtgctgtaa tacagttcca
     1681 gggcctgcgc atcgttcagc cgagctttgc cggcaatgac ggaatcgata tcgaacgaca
     1741 gcttgctcat acggtgatgg agtttatgag gtttacgggg tagaagaaag ttgggtcgcg
     1801 tttcgccgag gatttcaacg gaattgaaag ggtcgggtat cagtagcacg ggcgtcgggc
     1861 ccatgtcgag cggccttcgg cacgggcgag acgcccatgc cacatctatg aagtcctacc
     1921 tgctgattct cgtctgtgct ttctccatcg gcgccgtgct gaagcgaacg acgccaacgc
     1981 cgccaacgcc ggatgccgac gccactggcg tcagtaagct cgttcacctc gggtcgaatg
     2041 gaaagctcgt ttacgagccg tataccgagc atggcgacgt catccccgac ttctccaact
     2101 gcggttacat gggcggcggc gtgcggattc cggacgtcgc ggcgaagatc accctgcggc
     2161 cggaaacggg tagcggcgat gacaccgcgc gcattcagca ggcgatcgat caactgggca
     2221 acatgccgat cggtcccgat ggttttcgcg gcgcgctcgt gctctcccgc ggctactacc
     2281 acatcggcgg cagcttgaag atccacgcca gcggcatcgt cctgcgcggt gaaggcagcg
     2341 gcgaaggcgg caccgtgctc accgccaccg gcaacaaacc gcgggcgatg atcgttgtgg
     2401 ccagcgatgc caagcaggtc atcgtcgaat caaagcagtc gccggacgtg acggatgagt
     2461 atgtgccggt gggcgcgcgt tcgctcggcg ccgtcgatgc gtcgcagttc cacgttggcg
     2521 actgcatcat cgtccgccgg atcggcaacg cggcgtggat tcacgagctg ggcatggacg
     2581 acatgccgat ccagcgcagc ggcacgcgcg actggaaatc gttcgacatc gaatccgacc
     2641 gtgtgatcac ggccgtcaac ggcaaccaga tcacgttcga cgcgccgatc tgctgcgcga
     2701 tcgaaaagcg ctggggcggc ggcgaagtgg tgaagtacac cgatgccgag cgcatcggcc
     2761 agatcggcgt cgagaatctg cgggcgatct cggagttcga caaatcggtc gagaagaagc
     2821 tcggtgacac gtcgtactac gccgacgagc agcacgcgac ggacgcgatc tcgttcaaca
     2881 cctgcaagaa cgcgtgggcg cgaaacatcg tggcgatgcg cttcgtccac ggcgtggcca
     2941 agatggaacg cggatcgaag tggatcaccg tgcaggacag cgaagcgcgc gagccggtgt
     3001 cgatcatcac cggtggccgg cggtatccgt tcaacatcat ggggcaactg tgcctggtgc
     3061 tgcgctgccg gtcggacgag gaacggcacg cgttcgtgat gaacggctcg cacgatgccg
     3121 ggccggacgc gtttgtcgat tgcacgtcgg aacacaacca cgccgattcc gggccgcacc
     3181 aacgctggtc caccggcacg ttgtgggaca acgtagtcgg cgtgatgcac acgcaggacc
     3241 gcgaggacat gggcagcggc cacggttggg ccggcgcgaa cgatgtgtac tggaattgtt
     3301 cgggtcgcat cactatcgag cagccgccca ccgcccagaa cttcgccttc ggttttgtcg
     3361 ggcaaaagga caaggccgcg ttcacgcagc tgcatcatcc cgaggggtgg tacgaaagct
     3421 tcggcaagcg cgtccagccg ccaagtttgt acatgcagca gttgaaggat cgcctcggac
     3481 cacgggcgga cgagaacatt acgccgcccg gcagccatta aaactgtcgc gaattccggc
     3541 atttttctgg aaggattcgg tggttcgggt acaaacaccg gcgaaccaca acccctcgag
     3601 gaggctgcat ggccaagaaa gcggcaaaga agtctgcaaa gaaatccggg aaaaagtcgg
     3661 cgaagaagtc cgcgaagaag acgacgaaaa agtcgaccgg cagcacaatg aagaaggcgg
     3721 ccaaaaaggc cgcgtcgcgc gtgatgtcca gggcgaagga catgaagggc cggatgaagg
     3781 ccgcgccaaa aagcgcgacg cgcagcgcga acagcgccaa agccaagtca aaggggatgg
     3841 tgaagcgcgc gacgaagaag gtgaaccagg tggccaacgg cgtggccgac cgcgcggtgg
     3901 agatgagcga gcgggccgcc cccatcgccg agtcgatcgc cggcgcaatt gccggcgccg
     3961 ctggtgccgt cgtcggtacg atccaagcgg cgatcacgcc gagccagccg agcgggagct
     4021 cggacaactc gaatgagtct ggaaataaca acggttgaac gccggccggc gcgcgagcgc
     4081 ttgaggaaac ga
//
LOCUS       DATIMH010000753         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_468340, whole genome shotgun sequence.
ACCESSION   DATIMH010000753 DATIMH010000000
VERSION     DATIMH010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468340"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1269)
                     /locus_tag="VLI90_13225"
     CDS             complement(43..1269)
                     /locus_tag="VLI90_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15214.1"
                     /translation="MVHTHAAARTVSVLLVMFALAPFALAQDAAAPAGGDTQYISLTP
                     AEFSEKAPDLLNKDVEVAGDIQLPSMMSRKSVGTMVDPTSGKVLVQLMAQPGSPALEW
                     LVGGDRQANTHGVFARGKVVTSRYYSFPVLQLTDISKESKVKAAAEKKEQVQAVETKE
                     AAGLAKDVLPPHTNPTGEAAKAWLDRIPTTEASYDRAKSVYQNMQDHANWRGQAEVDD
                     NHALIRGPQRPANFETYYRGLRDTGLSNIFKKFPYQNSHTSWPRVMIVIEEKPRFNGG
                     MPSEFFHNSTKTETDYTWRYRATLWMDATHKIDIAPFNWAFSEMRYNTPYSEVEIWGR
                     TPKHNVPPAPGVEGTAGDPANPVPIATKYPLPDDPAYHRTFHYNTIMVGNLLLDMGFN
                     YNRQDGRVWFVEDIAK"
     gene            1557..1712
                     /locus_tag="VLI90_13230"
     CDS             1557..1712
                     /locus_tag="VLI90_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15215.1"
                     /translation="MRLPCVVVAGMNGIFDPAGGNGAGQISGKMRARPGPAFQAVRDA
                     TESTACA"
     gene            complement(1805..>2256)
                     /locus_tag="VLI90_13235"
     CDS             complement(1805..>2256)
                     /locus_tag="VLI90_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15216.1"
                     /translation="SACPNGSICVWNMSAVPSGAPRLLHRFKHGGEVRAIAFSPDGRL
                     LLAGGGDNTAQFWDVASGDPVGPPMRHDGAVIAVGFDPVNHLALTASEDGALRLWPIA
                     PTAETAADVTRWAETTTGMTSDAASNTRWLTVAEWWERTRSVSISRR"
BASE COUNT          377 a          715 c          705 g          459 t
ORIGIN      
        1 cccccacgcc gggttcggag gtactccgaa cccggcgtgg aatcacttgg cgatgtcctc
       61 cacaaaccac acgcgcccgt cctggcggtt gtagttgaag cccatgtcga gcagcaggtt
      121 gcccaccatg atcgtgttgt agtggaacgt gcggtgatac gccgggtcgt ccggcagtgg
      181 atatttcgtt gcgatgggaa cgggattggc cggatcaccc gcggtccctt ccacgccggg
      241 cgctggtggg acgttgtgct tgggcgttcg gccccagatt tcgacttcgc tgtatggcgt
      301 gttgtagcgc atctcgctga acgcccagtt gaacggggcg atgtcgatct tgtgcgtggc
      361 gtccatccac agcgtggcgc gatatcgcca ggtgtagtcg gtttcggtct tggtgctgtt
      421 gtggaaaaac tcgctgggca tgccgccgtt gaacctcggc ttttcctcga taacgatcat
      481 cacgcgcggc cagctggtgt gactgttctg gtaagggaac tttttgaaga tgttggacaa
      541 cccggtgtcg cgcaggccgc ggtaatacgt ctcgaaattc gccggccgct gcggcccgcg
      601 gatgagcgcg tggttgtcat caacctccgc ttggccgcgc caattcgcgt ggtcctgcat
      661 gttttggtag acgctcttgg cgcggtcata actggcttcg gtggtgggaa tgcgatcgag
      721 ccacgccttg gcggcttcgc cggtggggtt ggtgtgcggc ggcaacacgt ccttggccaa
      781 acccgccgct tctttggttt ccaccgcctg cacctgctcc ttcttctccg cggcggcttt
      841 gaccttcgat tctttcgaga tgtcggtcag ctgaagcacg gggaacgaat agtaccggct
      901 cgtgacgacc ttcccgcgcg cgaagacacc gtgcgtgttg gcctggcgat cgccaccgac
      961 gagccattcc aacgccgggc tacccggctg ggccatcaac tgcacgagca ccttaccgct
     1021 ggtcggatcg accatcgtgc cgacggactt gcggctcatc atcgacggca gctgaatatc
     1081 gccggcgacc tcgacgtctt tgttgagcag atccggcgcc ttctccgaga attcagcggg
     1141 ggtcaacgag atgtactgtg tatcgccccc cgccggagcg gcggcgtcct gagcgagtgc
     1201 gaatggcgcg agcgcgaaca taacaagaag caccgaaacc gtgcgcgcgg cggcgtgagt
     1261 gtgaaccatg tctctccctc gaattgtggt actgaatcca ctgctaagca acgcgcgacg
     1321 attatacgcg ggacgagcaa caaatttgaa gctttttacg cgttgcagag cgagcagggt
     1381 gtaactgagc tctgtggtcc agcccttctc tggtcccctc gcccggtact ccgggagagg
     1441 gttagggtga gggtgatttc gagtgccagc gactatcgaa gttcaaaatc accctcaccc
     1501 ctgccctctc ccggagacct gcggtcagca gtaccgggag agggggaagg agctacatgc
     1561 gattgccctg cgtcgtcgtg gcggggatga acggcatctt cgatcccgcc ggtggcaacg
     1621 gcgcggggca gatttcgggc aagatgcgcg ctcgtcctgg cccggcgttc caagcggtcc
     1681 gcgacgcgac cgaatcgacg gcttgcgcct gacgcccacc agcaggcctt ccggtcccgg
     1741 caaccgcgtc gcgcatcggt caaacgaatg cgttcatcaa attcgatgcc atcgcacctt
     1801 cgattcatct tcgactgatt gaaacgctcc gcgtccgctc ccaccactcg gccaccgtca
     1861 accatctcgt gttgctggcg gcgtcgctgg tcatcccggt cgtcgtctcc gcccagcgcg
     1921 tcacatccgc cgcggtttcc gcggttggcg cgatcggcca taaccgcagc gcgccatctt
     1981 cgctggccgt cagcgcaagg tgattgaccg gatcgaatcc cacggcgatg accgcgccat
     2041 cgtgccgcat cggcggaccg acggggtcgc cgctggcgac gtcccagaac tgcgcggtgt
     2101 tgtccccgcc gccggcgagg agcaatcggc catccgggct gaacgcgatg gccctcactt
     2161 cgccgccatg cttaaatcga tgcagcagcc gcggcgcgcc gctcggcaca gcagacatat
     2221 tccacacgca gatcgatcca ttcgggcacg cgctcg
//
LOCUS       DATIMH010000754         1691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4221454, whole genome shotgun sequence.
ACCESSION   DATIMH010000754 DATIMH010000000
VERSION     DATIMH010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4221454"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VLI90_13240"
     CDS             complement(<1..705)
                     /locus_tag="VLI90_13240"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HSV15217.1"
                     /translation="MIHRLITTSLVLCISTLAAAAESSGLDPAKIEQLTGLKGAMDAK
                     EGVFKVSYPRADIQATAAGVKITPPMGLTCWVAFKNVGGEHVMCMGDTVLTEDQVNPV
                     MSAALDNGLEVTALHNHFFWDSPKVMFMHIGGSGTQDQLAAAVGKVYETLKATAGGKA
                     ELTKADIDPAQTSLDPKKIDAILGVKGALAKGVYKATIGRTTQMMGHEMGNQMGVNTW
                     AAFVGSDDKAAVMGDFA"
     gene            complement(781..>1691)
                     /locus_tag="VLI90_13245"
     CDS             complement(781..>1691)
                     /locus_tag="VLI90_13245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV15218.1"
                     /translation="PTRLHELLRHTLEVCASDIHARRIRIDQQFDATTDLVIADSARL
                     GQVFWNLLKNAVKFSESGGTIVVRTSGIAGDGRAAPPGVRAEIIDNGVGIPANVLPRV
                     FDAFEQGDVRTTRQFGGLGLGLAISKAVVDMHGGRIRATSAGPGMGATFTVELNAASG
                     ALADKDARQSSPQDSRPVARARILLVEDHADTARILARLLHHAGYEAHAVHNIAAALD
                     AAAKQPFDLLISDIGLPDGNGFDLMAQIKSRHGISGIALTGYGMEDDMRRGREAGFVD
                     HVVKPVSIPQLEQVIQRVLQDEQRVR"
BASE COUNT          262 a          543 c          553 g          333 t
ORIGIN      
        1 cgcgaagtcg cccatgacgg cggccttgtc atccgatccc acgaacgcgg cccaggtgtt
       61 cacgcccatc tggttgccca tctcgtggcc catcatctgg gtcgttcgac cgatcgtcgc
      121 tttgtagacg cccttggcca gcgcgccttt gacgccgagg atcgcgtcga tctttttcgg
      181 atcgagcgac gtttgcgcgg gatcgatgtc cgccttggtg agctcggcct tgccgccggc
      241 cgtggctttt aatgtctcgt acaccttgcc gacggcggcg gcgagttgat cttgcgtgcc
      301 tgacccaccg atgtgcatga acatgacctt gggactgtcc cagaagaaat ggttgtgcag
      361 cgccgtgacc tccagcccgt tgtccagcgc ggcggacatg accggattca cctggtcttc
      421 ggtcaagacg gtgtcgccca tgcacatgac gtgctcgccg ccgacgttct tgaacgcgac
      481 ccagcaggtg agccccatcg gcggtgtgat cttgacgccg gcggcggtgg cttggatgtc
      541 ggcgcgggga tagctgactt tgaagacgcc ttctttggcg tccatcgccc ctttgaggcc
      601 cgtgagctgc tcgatcttgg ccggatcgag gccggacgat tcggcggcgg cggcgagggt
      661 ggagatgcaa agaacgagcg acgtcgtgat gaggcggtgg atcatgagcg actcctatga
      721 attgagcggg cacgctaacc gccgaagcgg ttagcccggt tcaaacttta ttcgaatgcg
      781 tcaccttacc cgttgctcat cctgcagcac gcgctgaatc acctgctcca actgcgggat
      841 gcttaccggc ttcacgacgt gatcgacgaa gcccgcttcg cggccgcggc gcatgtcatc
      901 ttccatgcca tagcccgtca gcgcgatgcc gctgatgccg tggcggcttt tgatttgggc
      961 catcaggtcg aacccgttgc cgtcgggcag gccgatgtcg ctgatgagca gatcgaacgg
     1021 ttgctttgcc gcggcgtcca gggcggcggc gatattgtgc acggcgtgcg cctcgtaccc
     1081 cgcgtgatgc agcagacgcg cgaggatgcg cgccgtgtcg gcatggtctt ccacgagcag
     1141 gatgcgcgcg cgagcgacgg ggcggctgtc ctgcggcgat gattgccgcg cgtccttgtc
     1201 ggcgagcgcg ccgctggcgg cgttgagttc aaccgtgaac gtcgcgccca tacccggacc
     1261 ggcgctggtc gcgcggatcc tgccgccgtg catatcgacg acggctttgc tgatggccag
     1321 ccccagcccc agaccgccga attggcgggt cgtgcggacg tcgccttgct caaaggcgtc
     1381 aaacacgcgc ggcaacacgt tcgccggaat ccccacgccg ttgtcgatga tctcggcgcg
     1441 aacgcccggc ggcgctgcgc ggccgtcgcc ggcaatgccg ctggtccgca cgacgatggt
     1501 gccaccggat tcgctgaact tcaccgcgtt cttcagcagg ttccagaaca cctgccccag
     1561 gcgagccgaa tcggcgatca ccaggtctgt ggtcgcatcg aactgctgat cgatccggat
     1621 gcgccgggca tggatgtcac tggcgcacac ctccagcgtg tggcgcagca attcgtgcag
     1681 ccgggtcggc t
//
LOCUS       DATIMH010000755         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_759695, whole genome shotgun sequence.
ACCESSION   DATIMH010000755 DATIMH010000000
VERSION     DATIMH010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759695"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VLI90_13250"
     CDS             complement(<1..748)
                     /locus_tag="VLI90_13250"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15219.1"
                     /translation="MPTILGIESTCDETAASVVIDGRDVRSNVVATQVDLHAKYRGVV
                     PEIASRAHIENILPVLREALDKAARGFADIDAIAVAHRPGLIGSLLIGVTAAKTLAWS
                     LGKPLIGIDHVHAHLYSVVLGEMQNDERRMQNDGRMADPHSAFCIDQSAFAGFPAVGL
                     VCSGGHTALYRVGSWTDVELIGSTIDDAVGEAYDKVAAILGLGYPGGPIIDTLAASGD
                     PTVVKLPRTYLDRESLDFSFSGLKTAVLYHV"
     gene            complement(808..2217)
                     /locus_tag="VLI90_13255"
     CDS             complement(808..2217)
                     /locus_tag="VLI90_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HSV15220.1"
                     /translation="MNREKLAWTVSICLLAVLAFQIPGTLAQRDDDYAFVRTLVDIHR
                     RVADNYVDVVDESKLRQGAIDGMLGELDPFSIYVPPARQEDFDRMLEGSFKGVGIQLD
                     QKDNGQVEVVSPIDGSPAFKAGIQAGDIILKVNGESIENLRIPELIKRIGGPLGSQVS
                     LTVHHATGEDATLTMTREEIKVPTVKGYERKQDTTWDYYVCDDPKIAYVRLTQFTPES
                     YDDLRKVLEGLLADGMKGLIFDLRFNPGGRLDQAVQIINMLVPKGETIVVTRGRNRPE
                     QRQESNGENTLPNFPMIVLVNEHSASASEIVSGSLMDNKRAVVVGERSYGKGSVQEVI
                     PLDEKGGELKLTVAYYYLPSGRLVHRRKDAKDWGVQPQIPVPMDPATEEKVAKERYEA
                     ELFKRPLPKSVTRPATTQANATTQPLAPTLDDTQLQAAINTMLGVIVFESQPGGKLPA
                     TNPAVIATPATQPATQPGA"
     gene            complement(2440..3219)
                     /locus_tag="VLI90_13260"
     CDS             complement(2440..3219)
                     /locus_tag="VLI90_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSV15221.1"
                     /translation="MRPLAYTESASAGTPLVLLHGFPFDARMWDDQLRSLSDVAHVIA
                     PDLRGFGRSTSTDPFTMESQADDVHALLQSIGCLPCILAGMSMGGYIALAHIKKYAQD
                     VRALVLIDSKAAADDAEGKRARDQMIELARAQGAKAVADKTQPKLIAADTPVKRPQVL
                     PRMREMMEQCPPLTVQHAMAAMRDRPDHTATLATIAVPTLIIVGEADAVTPPSVAEQM
                     RGKLRNAQLATIPNAGHMAVMEQPQRVNDAVREFVLCVVKT"
BASE COUNT          599 a         1059 c         1098 g          692 t
ORIGIN      
        1 gcacgtgata tagcaccgcc gtcttcagtc cgctgaagga gaaatcaagc gactcgcgat
       61 cgaggtacgt gcgcggcaac ttgacgaccg tcggatcgcc actcgccgcg agggtgtcga
      121 tgatcggccc gccgggataa ccgaggccga ggatcgcggc gaccttgtcg tatgcttcac
      181 caacggcgtc gtcgatggtc gaaccgatca actccacgtc cgtccaggaa ccgacgcggt
      241 acagcgcggt gtgaccaccg ctgcagacga gaccgacggc ggggaagccg gcgaatgcag
      301 attgatcaat gcagaatgca gaatgaggat cagccatccg tccgtcattc tgcattctgc
      361 gttcatcatt ctgcatttcc cccaatacga cgctgtacag gtgcgcgtgc acgtgatcga
      421 tgccgatcaa cggcttgccc agcgaccacg cgagcgtctt ggcggcggta acgccgatca
      481 gcagggagcc gatcaggccc gggcgatggg cgacggcgat ggcgtcgatg tctgcgaagc
      541 cgcgcgcggc tttgtccagc gcctcgcgca gcaccggcag gatgttttcg atgtgcgcgc
      601 ggctggcaat ctccggcacc acgccgcggt acttggcgtg caggtccacc tgcgttgcga
      661 caacgttgct gcgcacgtcg cggccgtcaa tgacgaccga cgccgcggtt tcatcgcagg
      721 tggattcgat gccaaggatg gtgggcatga aacacaattg acaattgaaa attgaccatt
      781 gaaaattttc aatggttccc gcttcagcta cgcccccggc tgcgttgccg gttgcgttgc
      841 gggcgtggcg atgaccgcag gattcgtcgc cggcaacttg ccgccgggtt gcgactcgaa
      901 cacgatcacg ccgagcatcg tgttgatcgc cgcctgcagt tgcgtgtcgt ccagcgtcgg
      961 cgccagcggc tgcgtcgttg cattcgcctg tgtcgtcgcg ggacgcgtca cgctcttggg
     1021 cagcgggcgc ttgaacagct cggcctcata ccgttccttc gccaccttct cctcggtggc
     1081 ggggtccatc ggcacgggaa tctgcggctg cacgccccag tccttcgcgt ccttccgccg
     1141 atgcaccaac cggccgctgg gcagatagta ataggccacc gtcagtttca gctcgccgcc
     1201 tttttcgtcc agcgggatga cttcctgcac ggagcctttg ccgtaggagc gctcgccgac
     1261 gacgaccgcg cgcttgttgt ccatcaagct gccggagacg atttcgctgg cgctggcgga
     1321 atgttcgttc accagcacga tcatcgggaa gttgggcagc gtgttctcgc cgttggattc
     1381 ctgccgctgc tccggccgat ttcgcccgcg ggtgacgacg atcgtctcgc ccttgggtac
     1441 cagcatgttg atgatctgta ccgcttgatc caagcgccca ccggggttga agcgcaggtc
     1501 gaaaatgagc cccttcatcc cgtcggcgag caaaccttcg agcaccttgc gaagatcgtc
     1561 ataagactcc ggcgtgaact gcgtgaggcg gacgtaggcg attttcggat cgtcgcaaac
     1621 gtaataatcc catgtcgtgt cctgcttgcg ctcgtagcct ttgacggtcg gaactttgat
     1681 ctcctcgcgc gtcatggtca gcgtcgcgtc ttcgccggtg gcgtgatgca cggtgagcga
     1741 cacctgcgag ccgagcgggc cgccgatcct tttgatcagt tcggggatgc gcaggttctc
     1801 gatcgattcg ccgttgacct tgaggatgat gtcgccggcc tggatgccgg ccttgaacgc
     1861 ggggctgccg tcgatcgggc tgacgacttc cacctgcccg ttgtccttct ggtccagctg
     1921 gatgccgacg cctttgaacg agccttcgag catgcggtcg aagtcttcct gccgcgcggg
     1981 ggggacatag atgctgaacg gatcgagttc accgagcatg ccgtcgatcg cgccctggcg
     2041 cagcttcgat tcatcgacga cgtccacgta gttgtcggcc acccggcggt ggatatcgac
     2101 cagcgtgcgg acgaaggcgt agtcatcgtc gcgctgcgcg agcgtgccgg ggatctggaa
     2161 ggcgaggaca gccagcagac agatgctgac ggtccaggcg agtttttcac ggttcatgcg
     2221 gaggctccgt aacacaggcg gaaatcgcga acaccgggat gttagtcgcg gaaacgagcg
     2281 ggagcaaaag aggggtgagg gggattgtga cgaatgataa tagccgcggg cgatagccag
     2341 cgggagcgct ggacagtcaa ccgggctgat attcttaaac ccccgctggc tgttgcctgc
     2401 ggctattaag gatttactcg cgcgcgtgtg ccgagcgcgt catgtcttga ccacgcatag
     2461 gacgaattcg cgcaccgcgt cattcacccg ttgcggttgc tccatcacgg ccatgtgacc
     2521 agcgttggga atcgtcgcca gttgcgcgtt gcgaagtttc ccgcgcatct gctccgcgac
     2581 gctcgggggt gtcaccgcgt ccgcttcgcc gacgatgatc agcgtcggaa ccgcgattgt
     2641 cgcgagcgtc gccgtgtgat cgggtcgatc gcgcatcgcg gccatcgcgt gctggaccgt
     2701 cagcggtggg cactgctcca tcatctcgcg catccgtggc aacacctgcg ggcgcttcac
     2761 cggcgtgtct gccgcgatga gcttcggctg cgtcttgtcc gcgaccgcct ttgcaccttg
     2821 cgctcgcgcg agttcgatca tctgatcgcg cgcccgcttc ccctcggcgt catccgcggc
     2881 ggctttacta tcaatcagca ccagcgcccg cacgtcctgc gcgtacttct tgatgtgcgc
     2941 cagcgcgatg tatccgccca tcgacatgcc ggcgaggatg cacgggaggc agccgattga
     3001 ttgcagcaac gcgtgcacgt cgtcggcttg cgattccatg gtgaacgggt cggtcgatgt
     3061 cgagcggccg aatccgcgca ggtccggcgc gatcacgtgg gcgacgtcgg ataagctacg
     3121 gagttggtcg tcccacatcc gggcatcgaa gggaaaaccg tgcagcaaaa cgagcggcgt
     3181 tcctgcggaa gcggactcgg tgtaagccag cggtctcata gcgagcggca taaatctgcg
     3241 acgcctcggc gtcaactggc aaacccatgt tgatgtcttg ccccctctcc cggtactccg
     3301 ggagagggct ggggtgaggg tgatttcgag tactagcggt tatcgaaatt caaaatcacc
     3361 ctcaccctca ccctaaccct gtcccggact accgggagag gggaccggag gtgcacgtcg
     3421 cggatgaaac attggctcac gttgattg
//
LOCUS       DATIMH010000756         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2199649, whole genome shotgun sequence.
ACCESSION   DATIMH010000756 DATIMH010000000
VERSION     DATIMH010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199649"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VLI90_13265"
     CDS             complement(<1..1496)
                     /locus_tag="VLI90_13265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSV15222.1"
                     /translation="MLGIVGLLLLIACANVANLLLARAVGRQKEIAVRLAVGASRMLL
                     IRQLLAESLTLALTGGALGIFFAWWTGSALLGLLANPADIPISASPDLRVFAFTFVLS
                     LLTGILFGLAPAWQATSPKLALTLKDQANNVSGGTGHVRLRKVLVVSQVALSLLMLIA
                     AALFTRSLHNLKNVDLGFRREHLLSFSVDPSLASYKADRIRRFAEEVQTRVAGVPRVQ
                     SAAIGTVTLIAEEENLYTISIEGYQPKEDENMNPMFDTVSPGFFHTLGIPLLAGRDFT
                     ARDRMGSPRVAVVNDVFAKQYFGNEDPIGRRFGMGRGPRDIEIVGVVRAAKYSRVDEK
                     LPREVYMPYAQEENPSSMIVYARTTDEPKATFGALRREVAALDAALPVTSLRTMDDQI
                     DQSLSSQRAMAALCAFFGILATLLAAIGLYGVMAYTVMRRTREIGIRLALGAGRGDLL
                     SLVLREVALLTLAGVVIAVPVALAATRLVRSELYGILPNDPLSIAAAAA"
BASE COUNT          316 a          550 c          485 g          267 t
ORIGIN      
        1 gccgcggcgg cagcgatact caggggatca ttcggcagaa tcccgtaaag ctccgaacgc
       61 accaggcgag tcgccgccag cgccacaggc accgcgatca ccaccccggc cagcgtgagc
      121 aacgccacct cacgcaacac cagcgacagc aaatcgccgc ggccggcccc aagcgccagg
      181 cgtatgccga tttcgcgggt gcgccgcatt accgtgtagg ccatcacgcc atacagtccg
      241 atggccgcga gcagcgtcgc cagaattccg aagaacgcgc acagcgccgc catggcgcgt
      301 tgcgacgaaa gcgactggtc gatctggtcg tccatcgtcc gcaggctggt gacggggagc
      361 gcggcgtcga gcgccgccac ctcgcggcgc agtgcgccga atgtcgcttt gggctcgtcc
      421 gtggtgcgcg cgtacacaat catcgaactc gggttctcct cctgcgcata cggcatatag
      481 acttctcgag ggagtttctc atcaacgcgc gagtacttgg cggcgcgcac cacgccgaca
      541 atctcgatgt ctcgcggccc gcgccccatg ccgaaacgcc gcccgatcgg atcttcattc
      601 ccgaagtatt gcttcgcgaa cacgtcgttg acgacggcca cgcgcggcga ccccatacga
      661 tcgcgcgctg tgaagtcgcg gcccgccagt agcggaatcc caagcgtgtg gaaaaacccg
      721 gggctcaccg tgtcgaacat cgggttcatg ttctcatctt ccttgggctg gtagccctcg
      781 atggagatgg tatatagatt ttcctcctcg gcgatcaacg tgaccgtgcc gatcgcggcg
      841 gattggaccc gcggtacgcc ggccacccga gtctgcacct cctccgcgaa gcggcgtatg
      901 cgatccgcct tgtacgacgc cagcgacggg tcgacgctga agctgagaag gtgttcgcgc
      961 cggaagccca gatctacgtt cttcaagttg tgcagcgagc gcgtgaacag cgctgccgct
     1021 atcagcatca gcagcgagag cgccacctgc gagaccacca gcaccttgcg cagcctcaca
     1081 tggcccgtgc cgcccgagac gttgttggcc tgatccttta acgtaagcgc cagcttcggc
     1141 gatgttgcct gccacgcggg cgcgaggccg aacagaatcc ctgtcagcag tgacaggacg
     1201 aacgtgaacg caaagacccg caggtcaggc gatgcgctga tgggaatgtc cgccggattc
     1261 gccagcaacc ccagcagtgc gctcccggtc caccacgcga aaaagattcc gagcgctccg
     1321 cctgtcagcg caagcgtcag actttcggcc agcaactggc ggatcagcaa catacggctt
     1381 gcgcccacgg cgaggcgcac ggcgatttcc ttctgccgcc ccaccgctct cgccagcagc
     1441 aggttggcta cattggcgca ggcgatcagc agcagcaatc cgacgatgcc cagcaagatg
     1501 agcagcggat ccgagagctg ctctcgcaag tccgatactc ccttgcccgc gggaacgaaa
     1561 atcagcggtt tcgtgatgaa tgccgtgcgg ctgcgctccg tacggaactt catgctct
//
LOCUS       DATIMH010000757         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5381895, whole genome shotgun sequence.
ACCESSION   DATIMH010000757 DATIMH010000000
VERSION     DATIMH010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5381895"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1371
                     /locus_tag="VLI90_13270"
     CDS             73..1371
                     /locus_tag="VLI90_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HSV15223.1"
                     /translation="MESPEAEQRGALAWWIDIPLYLRIIGGLILGIILGLTLKAHPTV
                     AGVSSDKILNGFGAITGLILKLLGALAPPLILVAVLDTLIKARLQGGVARRMIWLLVT
                     NTLAAILIGLITANVVQPGHRAKLDVAHDGAAMMPHGNPWEALLGNVPDSLLRPLVDN
                     NVIGVILIGAAFGIALRRLDGDDRRIAEQMVGVAFRAIAIVLHWIIELVPLGVFGIIA
                     RVVGTSGFAPFYALGWFVLAVVLGLLLQLAFYLTRVRVSSWVSPLKLLSGMRDAIVMA
                     FSTDSSTATMPVTYRCLRDNVGLRDESARMGALVGTNFNNDGTALYEAMSALFVAQLI
                     PIHLSFAQQLMVVLTSIVASVGAAGIPEAGLVTMTLVFSAVGLPKEYIAMLLTVDWFL
                     DRCRTVINMMGDTCVACLLDGKQQAIESERAMVAEVVAVV"
     gene            complement(1471..1749)
                     /locus_tag="VLI90_13275"
     CDS             complement(1471..1749)
                     /locus_tag="VLI90_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15224.1"
                     /translation="MDFINPVAGSLLQSTSVQRQQSVDKQRQVRRAQALERDVAAQDD
                     QLEHQVESSEELPPVNDEHPEHEQQKKKKRGMDQGADDEDPPHLDLTA"
     gene            2005..>2665
                     /locus_tag="VLI90_13280"
     CDS             2005..>2665
                     /locus_tag="VLI90_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15225.1"
                     /translation="MADVLDQSEVDALLAAVDSGQVSNDAPPQVYSGGGKGGATQVDV
                     QTYDFKRPERVSKDQMRALEALHEGFGRNFGAALSGYLRTIIEVSVAHIEQLTYSEFI
                     HSLPNPTCFNLLKAEQLDGQLCLEISPLIIYPIIDRLLGGSSSDLFIPQRPLTQIEQR
                     LVQRITDRATQHLSEAWSNLTPLTFTVNDFESNPQLVQIVPPNETVVVVGFELKMGNR
                     AG"
BASE COUNT          447 a          820 c          865 g          533 t
ORIGIN      
        1 ggtcttcgga ctacctcaga caccggcgcc gtgggttctg gcgcggggcg cgcattaagg
       61 gcaacacact cgatggaaag tcccgaggca gagcaacgcg gtgcgctcgc gtggtggatc
      121 gacatcccgc tatatctccg catcatcggc ggtctgatcc tcggcattat cctcggtctt
      181 acactgaagg ctcatcccac cgtcgcgggg gtttccagcg acaagatcct aaacggcttt
      241 ggcgcgatca ctggtttgat tctcaagctg ctcggcgccc tcgcaccgcc gctgattctg
      301 gtcgcggtgc tcgacacgct gatcaaggcc cgcctgcagg gcggcgtcgc tcggcgcatg
      361 atctggctgc tcgtcaccaa caccctggcg gcaatcttga ttggattgat caccgcgaac
      421 gtcgttcaac cgggtcatcg cgccaagctc gacgtggcgc acgacggcgc cgcgatgatg
      481 ccgcacggca acccgtggga ggcgctgctg ggcaatgtgc ccgacagcct gctgcgcccg
      541 ctcgtggaca acaacgtgat cggcgtgatc ctgatcggcg cggccttcgg tatcgcccta
      601 cggcgactgg acggtgacga tcggcggatt gccgagcaga tggtgggcgt cgcgtttcgc
      661 gccatcgcca tcgtgctgca ctggatcatc gagctggtgc cgctgggcgt gttcggcatc
      721 atcgcgcgcg tcgtcggcac cagcgggttt gcgccgttct acgcgctggg ttggttcgtg
      781 ctcgcggtcg tgctggggtt gctgctgcaa ctggcgttct acctgacgcg cgtgcgcgtc
      841 agttcgtggg tcagtccgct gaagctgctg tccggcatgc gcgatgccat tgtgatggcg
      901 ttcagcaccg acagttccac cgcgaccatg ccggtgacgt accgctgcct gcgcgataac
      961 gtcggcctgc gcgatgagtc agcccgcatg ggcgcgctgg tcggcacgaa cttcaacaac
     1021 gacggcaccg cgctttacga agcgatgagc gcgctgttcg tcgcgcagct gatccccatt
     1081 catctcagtt tcgcccagca attgatggtc gtgctgacgt cgatcgtcgc gtcggtcgga
     1141 gccgcgggga ttcccgaagc ggggctggtg acgatgacgc tggttttctc cgcggtggga
     1201 ttgccgaagg aatacatcgc gatgctgctg acggtggatt ggttcctgga ccgctgccgc
     1261 acggtcatca acatgatggg cgacacgtgc gtggcgtgcc tgctggacgg caagcagcag
     1321 gcgattgaat cagaacgtgc gatggtagcc gaggtggttg cggtcgtttg acttgaccac
     1381 gccgggttcg gaggtactcc gacgacccgg atgcgttctg gagaagaaga atctcgcttc
     1441 gtcgcgagta cgctccgaac ccggcgtggg tcaggcggtc aagtcgaggt gcggcggatc
     1501 ttcatcatcc gcgccctggt ccatcccccg ctttttcttc ttctgctgct cgtgctcggg
     1561 atgttcatcg ttcaccggcg gcagctcttc ggagctttcc acctggtgtt ccagctggtc
     1621 gtcctgggcg gcgacgtcgc gctccagcgc ctgcgcccgg cggacctgcc gctgcttgtc
     1681 cacagactgc tgccgctgca ccgaggtgct ttgcaacagc gaaccggcta cggggttgat
     1741 gaagtccatg ggcgtgaggc gcgcgcggcc cgcgcggatt gggcggcgcg atatcattaa
     1801 tgtcggcaag aatcgacagg cgtttgagcg aaacctgccc aatgggctgt ggttttgctg
     1861 tagccgcgac gactctgcat cgcctctttc cccgcggtga acaggcgacg caggcgtcgc
     1921 ggctacgggg gtaatcgact ccgcctctag aaaaaccgcc ttggggtccg ataatgaacc
     1981 aatcggcaag gaggccggcg ccgcgtggct gatgtactgg atcaatctga agtagacgcc
     2041 ctcttggccg ctgtggacag cggacaggtg agcaatgacg ccccgccgca ggtctacagc
     2101 ggcgggggca agggcggcgc gactcaagtc gacgttcaaa cctacgactt caaacggccc
     2161 gagcgcgttt ccaaggatca gatgcgggcg ctggaagccc tgcacgaggg ttttggccgg
     2221 aactttggcg cggccctctc cggttacctc cgcacgatca tcgaagtctc ggtcgcgcac
     2281 atcgaacagc tgacttacag cgaattcatc cactctctgc cgaatccgac gtgtttcaac
     2341 ctgctaaagg cggaacagct ggacgggcag ctgtgtctgg agatcagccc gctgattatt
     2401 tatccgatca tcgatcgcct gctcggcggc agcagttccg acctgttcat cccgcagcgg
     2461 ccgctcacgc agatcgagca gcggctggtg cagcgcatca ccgatcgcgc gacgcagcat
     2521 ttgtcggaag catggagcaa cctgacgccg ctgacgttca cggtgaacga tttcgaaagc
     2581 aacccgcagc tggtgcagat cgttccgccg aacgaaaccg tggtcgtcgt gggcttcgag
     2641 ctgaagatgg gcaatcgcgc cggca
//
LOCUS       DATIMH010000758         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4510362, whole genome shotgun sequence.
ACCESSION   DATIMH010000758 DATIMH010000000
VERSION     DATIMH010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4510362"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..998
                     /locus_tag="VLI90_13285"
     CDS             228..998
                     /locus_tag="VLI90_13285"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HSV15226.1"
                     /translation="MLLLIVLSSSPLLAQVMVTPATAPATTAATTQPNNGPADEPAAK
                     IGRDGKADTRFMELHRSFLWRGKEAPMGVLFLGDSITEGWMTRGASVWRERYWKYVPA
                     DFGIGGDRTQHVLWRIDNHELDNIAPKVVVLMIGTNNTANYTAEEITAADKRIVQQIH
                     EKLPETKLLLLAIFPRGADPADPKTAGLREKIRQVNVELAKLDDGDKTRYLDIGPKFL
                     DGEGKITKEMMPDALHPTKKGYVVWADAMQPLLDEMMK"
     gene            1297..2640
                     /locus_tag="VLI90_13290"
     CDS             1297..2640
                     /locus_tag="VLI90_13290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSV15227.1"
                     /translation="MSAEFTPKAIWPLTKRTFNEWLEDKVPKMSAALAYYITVSLAPL
                     LVITLKVVGVFFGAKAASTQVHDQIASLTGGQIADAMQDIIARAGQPGSGTIATILSI
                     VIALFGASGVFGELQDSLNTIWEVRPKPNRGIWGIIRDRFLSMTMVLGCAFLLLVSMF
                     ISTAITGVSGALLHSIFGGDGMTTKIIGFIIDLIISTAVITALFAAIFKVLPDVKISW
                     GDVAMGAFVTAVLFQIGKYALGVYFAKAAPGSAYGAAGSIVALLLWVCYSGYILFFGA
                     EFTQVYANEYGTRIVPSKNAEPLTEDMRRQAGIPHDQPKATTREGLTSSRPEGARPAT
                     GAAPKPHPQPMPPRRRRYRRQPTPRVTADHHSYVVRYLPVLAGVVLGRFAWKRYHEAQ
                     PTAQSLKNQWTDAGLKWKKLVSLFGSDTPTRYTKTHGPAGRKGPSWSDLIRQTAK"
BASE COUNT          515 a          829 c          913 g          495 t
ORIGIN      
        1 ccgggtcgtc ggagtacctc cgaacccggc gtggccggtt cttaaccatt cctgcaaaag
       61 tgtcggttcg tcagcgcagt accgggagag ggggaaggag cgatgtgcgc tcgctcccca
      121 cgtgttaggc gcggcgacga aagtgcactt gccattcgac gcatggcccg gctaaaagtg
      181 cgggcatgca aatcatcaat cgcgcttcgg atcgatgccg ccttcttgtg ctgctcttga
      241 tcgtgctgtc gtcttcgccg ctgttggcgc aggtgatggt cacgccggcg acagcgccgg
      301 ccaccactgc cgcgacgacg cagccgaaca acggaccggc cgatgaaccg gcggcgaaga
      361 tcgggcgcga cggtaaagcc gacacgcggt tcatggagtt gcatcgctcg ttcctttggc
      421 gcggcaagga agcgccgatg ggcgtgctgt tcctgggcga ctcgatcacc gaaggctgga
      481 tgacccgcgg cgccagcgtg tggcgcgagc gatattggaa gtacgttccc gccgacttcg
      541 gcatcggcgg cgatcgcacg cagcacgtgc tttggcggat cgacaatcac gagctggaca
      601 acatcgcgcc gaaggtggtc gtgctgatga tcggcacgaa caacaccgcg aactacacgg
      661 ccgaagagat cacggcggcg gacaagcgga tcgtgcagca gatccacgag aagctgccgg
      721 agacgaagct gctgctgctg gcgatcttcc cgcgcggggc ggatccggcg gatccgaaga
      781 cggcggggct gcgcgaaaaa atccggcagg tgaacgtcga gttggcgaag ctggacgacg
      841 gcgacaagac gcgctacctg gacatcggcc cgaagttcct ggacggcgag gggaaaatca
      901 cgaaggagat gatgcccgat gcgctgcacc cgacgaaaaa gggatacgtg gtttgggcgg
      961 acgcgatgca gccgttgttg gatgagatga tgaagtgatc gatggtcatc ctgagcgcag
     1021 gcggggctcc gccggagtcg aaggatctag ccggcgcgtc ggtgaacgat caatcgcgcg
     1081 gatcgcgaat tagtcgcttg cggctttgca gacggacaag ccgcggtcat tggtttgcta
     1141 agccgcaagc gactaccgcg ccgtcctaca cgaccatcgt tcacctggtt cccgttagct
     1201 ccatcgactc cggcggaata ccgcctgcgc tggaggatga ccccgtaggc acagccgttg
     1261 catccgtcac ctcccgtaga cagcccggga gattccatgt ccgcagaatt cacacccaag
     1321 gccatctggc cgctcaccaa acgtaccttt aatgaatggc tggaagacaa ggtgccgaag
     1381 atgtcggccg cgctggcgta ctacattacg gtatcgctgg ccccgctgct ggtcatcacg
     1441 ctgaaggtgg tcggcgtctt tttcggtgcc aaggctgcca gcacgcaggt gcacgatcag
     1501 atcgcttcgc tgacgggcgg acaaatcgcc gacgcgatgc aggacatcat cgcccgcgcg
     1561 ggacagccgg gcagcggcac gatcgccacc atcctcagca tcgtcatcgc cctcttcggc
     1621 gccagcggtg tctttggcga gttgcaggat tcgttgaaca cgatctggga agtccgcccg
     1681 aaacccaacc gcggcatctg gggcatcatc cgcgaccgct tcctctccat gacgatggtg
     1741 ctcggctgcg cgtttttgct gctggtgtcg atgttcatca gcaccgccat caccggtgtt
     1801 tccggcgcgc ttctgcactc gatcttcggc ggcgatggga tgaccaccaa gatcatcggg
     1861 ttcatcatcg acctgatcat ctcgaccgcc gtcatcaccg cgctgtttgc cgccatcttc
     1921 aaggtgctgc cggatgtgaa gatttcctgg ggcgacgtgg ccatgggcgc gttcgtcacg
     1981 gcggtgctgt tccagatcgg caaatatgcg ctgggcgtct atttcgccaa ggccgcgcca
     2041 ggttcagcct acggcgcggc ggggtcgatc gtcgctctgc tgctgtgggt atgttactcg
     2101 gggtacatct tgttcttcgg cgcggagttc acgcaggtgt acgcgaacga atatggcacg
     2161 cggatcgtgc cgtcgaagaa cgccgagccg ttgacggaag acatgcggcg gcaggcgggg
     2221 attccgcacg atcaaccgaa ggcgacgacg cgcgaggggt tgacgtcgtc gcgacccgaa
     2281 ggcgccaggc ccgcgacggg cgcggcgccg aagccacacc ctcagccgat gccgccgcgg
     2341 cgccggcgct accgtcgcca gccgaccccg cgggttaccg ccgatcacca ttcgtacgtc
     2401 gtccgctacc tgccggtgct ggccggggtg gtgctggggc ggttcgcgtg gaagcgctat
     2461 cacgaagcgc aaccgacagc gcaatcattg aagaaccagt ggacggacgc gggcttgaag
     2521 tggaagaagc tcgtatcgct atttggctcc gatacaccga cgcgttacac gaagacccac
     2581 gggccggcgg ggcggaaggg gccgagctgg agcgacttga tccggcagac ggcgaagtag
     2641 ccggaggcaa ttccatctct atggggattc tcgatctttc agatcggttg tgtagtggaa
     2701 tgcggcgcgc tgcggcgctt ttaggggtca aatgcggcgc gccggagctt cc
//
LOCUS       DATIMH010000759         1953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5093175, whole genome shotgun sequence.
ACCESSION   DATIMH010000759 DATIMH010000000
VERSION     DATIMH010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5093175"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /gene="accD"
                     /locus_tag="VLI90_13295"
     CDS             complement(<1..895)
                     /gene="accD"
                     /locus_tag="VLI90_13295"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HSV15228.1"
                     /translation="MAWFKKTRKPIEAKEKPSRVPEGLWVKCPGCAQAIYNKDLAANL
                     NVCPKCAHHFRVNAAERLRMLFDGDWVEHDPGLVSTDPLNFTDTKSYKARLKAGQAST
                     GIKDAVIVGSGTIDGVETIVAAQEYAFIGGSMGVVVGEKITRAIEKAIERRCPMIIVC
                     CSGGARMMEGALSLMQMAKISGALARLDRAKLPYIAVLTDPTTGGVSASFAMLGDLNI
                     AEPKAQIGFAGPRVIEQTIRQKLPEGFQRSEFLIEKGMLDLVVDRREMKATIAKALRF
                     MGAVPAPKPEAAAAISPVPEPA"
     gene            complement(914..1555)
                     /locus_tag="VLI90_13300"
     CDS             complement(914..1555)
                     /locus_tag="VLI90_13300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HSV15229.1"
                     /translation="MQFRYIAVEGPIGAGKTALAERLATRLDATLVLEDTENPFLTDF
                     YADRPGSALQAQLFYLLQRHRQQTTLQQADLFSQTTVADYLFDKDKIFAYLNLDDNEL
                     FIYQRLYDLLTRDVPGPDLVIYLQTPTDVLLRRIRSRRQDPEVDGYEPDPEYLRELNE
                     AYHHYFFHYEATPLLVVETSQFDSDASDEALDDLVRQIGQMGAGTRYYVDRTK"
     gene            1692..>1953
                     /locus_tag="VLI90_13305"
     CDS             1692..>1953
                     /locus_tag="VLI90_13305"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSV15230.1"
                     /translation="MSWILRLEDSRVLRDPVPETPPPAPPPVDPRGKSAAAAPVPPPP
                     LVPDLTRLITDKEARVRRRAALAIGRVGLVEGVPPLVSLLADS"
BASE COUNT          330 a          623 c          612 g          388 t
ORIGIN      
        1 ccgccggttc cgggaccggc gaaatggcgg ccgctgcttc aggcttcgga gcaggcactg
       61 cccccatgaa gcgaagcgcc ttggcgatcg tcgccttcat ctcccgccga tcgaccacca
      121 ggtccagcat tcctttttcg atgaggaact ctgagcgctg gaagccttcg ggcaatttct
      181 ggcggatggt ctgctcgatg acgcgcgggc cggcgaaacc gatctgggcc ttcggctcag
      241 cgatgttgag atcgcccagc atcgcgaaac tcgcgctgac gccgcccgtc gtcggatcgg
      301 tgagtactgc gatgtacgga agctttgcgc gatccagacg tgcgagtgca cccgagatct
      361 ttgccatttg catcaacgag agcgcgccct ccatcatccg ggcgccgccg gagcagcaga
      421 cgatgatcat cgggcatcgg cgctcgatcg ccttctcgat ggctcgggtg atcttctcgc
      481 caacgacgac gcccatgctt ccgccgatga acgcgtactc ctgtgcggcg acgatcgttt
      541 cgacgccgtc gatcgtgccg gaccctacga tgaccgcgtc cttgataccg gtggacgcct
      601 ggccggcttt gagccgcgct ttgtaggact tggtgtcggt gaagttcagg ggatcggtgg
      661 aaaccaggcc gggatcgtgc tcgacccagt caccgtcgaa cagcatccgg aggcgctcag
      721 ccgcgttcac gcggaagtga tgcgcgcact tggggcaaac gttcaggttt gcggcgagat
      781 ccttgttata gatcgcctgc gcacagccgg gacacttcac ccacagcccc tccggaactc
      841 ggctgggctt ttccttggct tctatgggtt tgcgcgtctt cttgaaccac gccataaacg
      901 atcagcttac cactcacttt gtccggtcga cgtagtaccg ggtgccggcg cccatctggc
      961 cgatctgccg tacgaggtcg tcgagcgctt cgtcgctcgc gtctgaatcg aactgagagg
     1021 tttccacgac gaggagcggc gtcgcctcat agtggaagaa atagtggtga tacgcctcat
     1081 tgagttcgcg caggtactcg gggtcgggct cgtatccgtc cacttccgga tcctgccgcc
     1141 ggctgcggat gcggcgaagc aacacgtcgg tcggcgtctg cagatagatc accagatccg
     1201 gtcccgggac gtcgcgcgtg agcagatcgt agagacgctg gtagatgaac agttcgttat
     1261 cgtcgaggtt caggtacgcg aagatcttgt ctttgtcgaa caggtaatcg gcgacggtgg
     1321 tctggctgaa aagatcggcc tgctgcagcg ttgtctgctg gcgatgacgc tgcaggagat
     1381 agaacaactg cgcctgcaac gccgatcccg gtcggtctgc gtagaagtcg gtgagaaacg
     1441 ggttctcggt gtcttcgagc accagcgtcg catcaagcct ggtcgcgagt cgctctgcga
     1501 gcgcagtctt gcccgcgccg atcggtcctt cgaccgcgat gtagcggaac tgcaaggcga
     1561 cccgatgata tcatcgcccc gtgacgcgta cgacgatcgc gctgctgctc acgctcggcg
     1621 cgtgcgcatg cgccagcgct cctgccgcaa ctccgccagc agcacctcca gggccaccgt
     1681 tcgaacagaa aatgtcgtgg atcctccggt tggaggactc gcgggtactg cgcgatcctg
     1741 ttcccgaaac tccaccgccg gcgccgccgc ccgttgatcc acgcggcaag agcgcggctg
     1801 ctgcgcctgt accaccgccg ccgctggttc cagatctcac gcgcctgatc accgacaagg
     1861 aagcgcgcgt gcgccgtcgg gcagcgctcg ctattggccg cgtcggcctt gtcgaaggcg
     1921 tgccgccgct cgtctcgctg cttgcggact ccg
//
LOCUS       DATIMH010000760         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4804583, whole genome shotgun sequence.
ACCESSION   DATIMH010000760 DATIMH010000000
VERSION     DATIMH010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4804583"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1506
                     /locus_tag="VLI90_13310"
     CDS             136..1506
                     /locus_tag="VLI90_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15231.1"
                     /translation="MSRPRRRSLALRSLALGFAAAVFAATPAVVFAEDAPVVDNSKVS
                     FAGQINSNAVYVRSGPGENYYATLKLDKGATVTVVGMKFDWLKIVPPEGSFCYVAKAY
                     VEKRGDGTVGRVTKPDLNVRAGSDLNAMKTTVQTKLDQEQDVTIVGEQDEYFKIKPPT
                     GAYLYVNKQFVDPVKPLPPVATANPSTGKESEPISSAPMSPIVSKTPDAPAPAAAPTT
                     KPANEQAAAAAPTTQGSEQQAAAPASTQPAAPSAEAQFDTLESAFLDATNQPLDKQPI
                     DDLLTKYTELSKNSDLPISMERIAEMRIGTLKLRADARTQYLDAQKMQAEAKERQQAL
                     KAEQEELAQRVKDNEVQIYAAIGTLRTSSLQQAGQTLYRLTDPGTGRTLLYVRSTDPK
                     IAGMLNQFIGVRGEITEDTTMSLKIITPTETTSVDQSKVGTTITAQVIPPTLLPKAPT
                     ASISGN"
     gene            1810..2487
                     /locus_tag="VLI90_13315"
     CDS             1810..2487
                     /locus_tag="VLI90_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15232.1"
                     /translation="MPSQSRDLRQLKAMLEQQYPRAYRIAVALCGDPLLARAVTRDVL
                     RQSLRIHQQWTDDLDAARWFAHHTVLTARQAIVGQAPDPTNDAALALTNDPLFTTILR
                     TTRFIPPQQREAFLLHHGEKFDLRQLATAMDCSADAAANHLVAATNALRPVAAGRLAD
                     FTNALPQLLARLVPPRESIAMEAVRQARRYIWPRRLMRWVGWPLVMLLLATAMYFGWR
                     LWQMLVI"
BASE COUNT          506 a          858 c          767 g          411 t
ORIGIN      
        1 cgctccagtt ttacctccag aaatcagctc gtttcttcgc aaagatcggg cgatttactg
       61 gccgctcaat ggattgagtc atactcgcga ttccggttac acaccggccg ggtttaccgc
      121 acatggaggt gcgcaatgtc acgtccccgc cgccgttcgc tggctcttcg ttcattggct
      181 ctgggttttg ccgcggccgt tttcgcggcg acgccggctg tcgtcttcgc cgaagacgcc
      241 ccggtcgtcg ataactccaa ggtgagcttc gccggccaga tcaattccaa cgccgtctac
      301 gtccgctccg ggcccggcga gaattactac gccacgctca agctggacaa gggcgcgacc
      361 gtcacggtcg tgggcatgaa gttcgactgg ctcaagatcg tcccgcccga aggctcgttc
      421 tgctacgtcg ccaaggcgta cgtcgagaag cggggcgacg gcaccgtggg tcgcgtgacc
      481 aagccggacc tgaacgtccg cgccggcagc gatcttaatg cgatgaagac gacggtgcag
      541 acgaaactgg atcaagaaca ggacgtgacg atcgtcggcg agcaggacga atattttaag
      601 atcaagccac cgaccggcgc gtacctgtac gtgaacaagc agttcgtcga tccggtgaaa
      661 ccgctgccac cggtggcgac agcgaacccg agcacgggca aggagagcga gccgatttcc
      721 agcgcgccga tgtcaccgat cgtcagcaag acgcccgatg cccccgcgcc ggccgcggcg
      781 ccgacgacca agcccgccaa cgagcaagcc gccgcggctg cgccgaccac gcaaggcagc
      841 gagcaacagg ccgcggcccc ggcgtccacg caaccggccg ccccgtccgc cgaggcgcag
      901 ttcgatacgc tagaaagcgc gttcctggac gcgacgaatc aaccgctcga caagcagccg
      961 atcgacgatc tgctgacgaa gtataccgag ctgtcgaaga acagcgacct gccgatctcg
     1021 atggagcgga tcgcggagat gcgcatcggc accctcaagc ttcgcgccga cgcccgcacg
     1081 caatatctcg acgcgcagaa gatgcaggcg gaagcgaagg agcgccaaca ggcgttgaag
     1141 gccgagcagg aagagctggc ccagcgcgtg aaggacaacg aggtgcagat ctacgccgcc
     1201 atcggcacgc tgcggaccag cagcctgcaa caggccgggc agacgctgta tcgcctgacc
     1261 gaccccggca ccggccgcac gctgctgtac gtgcgatcga ctgacccgaa gatcgcgggg
     1321 atgctgaacc agttcatcgg cgtgcgcggt gagatcaccg aagacacgac gatgagcctg
     1381 aagatcatca cgccaaccga aacgacgtcg gtcgatcagt ccaaggtagg cacgacgatc
     1441 accgcgcagg tgatcccgcc cacgctgctg cccaaggccc cgaccgcgag catcagcggg
     1501 aactgactta atttacgata gacattgcga cgtgaagccc ggcgcgcgcg gcgtggttgt
     1561 tgtagcaaca tgcttctcga acgcacgagg acatggttgc ttaagcaacc acgccccgcg
     1621 ggcttttagt cctcgacgca tttgttgcgc ctgcgcgatt cttcattcat tactctgcat
     1681 ccaattccat ccgggtcgtc ggaatgcttc cgaaccggcc atcgaaattc tgtatcaccc
     1741 tcaacccagc cccctcccag gagcaggcgg gcgagggagt aagcagctgt tccattacaa
     1801 tccagaagaa tgccatcgca atcgcgcgat ctccgccagc tcaaagcgat gctcgaacag
     1861 cagtaccctc gcgcctaccg catcgcggtc gcgctctgcg gcgatccact gctcgcccgc
     1921 gcggtcacgc gcgatgtcct gcgccaaagc cttcgcatcc accagcagtg gacggacgac
     1981 ctcgatgccg cccgctggtt cgcgcatcac acagtgctga cggcgcgaca ggcgatcgtc
     2041 gggcaggcgc cggacccgac aaatgacgcg gcgctggcac tgacgaacga tccactgttc
     2101 acgacgatcc tgcgcacgac ccgcttcatc cctccgcagc agcgagaggc atttctgctt
     2161 caccacggcg aaaaattcga cctgcggcaa ctcgccaccg cgatggactg cagcgccgac
     2221 gccgccgcga accatctggt ggccgcgacg aacgccttgc gccccgtcgc cgcggggcgg
     2281 ctggctgact tcaccaacgc gctgccgcag ttgctggcgc gactggtccc cccgcgggag
     2341 tcgatcgcga tggaagcggt gcgtcaggcc cgccgctaca tctggccgcg gcgcctcatg
     2401 cgctgggtcg gttggccatt ggtaatgctg cttctcgcca ccgccatgta tttcgggtgg
     2461 cgcctgtggc aaatgctggt gatttgatgc acgtgtcgct cagcccccac gccgggttcg
     2521 gaggtgctcc gacgacccgg at
//
LOCUS       DATIMH010000761         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5383082, whole genome shotgun sequence.
ACCESSION   DATIMH010000761 DATIMH010000000
VERSION     DATIMH010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5383082"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VLI90_13320"
     CDS             complement(<1..548)
                     /locus_tag="VLI90_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15233.1"
                     /translation="MLTCLAVSSRAVAADDGALQWQAKDVRLVHLYDNHAQVKVSDGL
                     TVMADGSDLAITLGSRAPWSDVMLTERCPFDPRGVLRVDVADIKGGDTSVQVVCWDDA
                     GNNLQMFDIFRAINKPGIYEMPLAAYRSVFPDATRQISFRLVIGGRSGAQARYRSVAY
                     GISTTAVVPPAVRTIEKYEPAKA"
     gene            complement(593..1426)
                     /locus_tag="VLI90_13325"
     CDS             complement(593..1426)
                     /locus_tag="VLI90_13325"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSV15234.1"
                     /translation="MSQANDLRVMSYNIRNTQAPDGPNHWVHRRDVWKASVRAFSPDL
                     LGLQEVWVDQHDELREDLGDYGFAGVARDDGKRSGEWAMILYRLERFELLDVGNFWLS
                     ETPAVAGSKSWDSKHVRICSWVRVRDRLNGATLLHANTHLDDMGTRARAEGAKLIRRV
                     LPELAGGGVVVLTGDFNSTEVDEPYAALLSRDTSSDLQLSDSYRLVHPQASANESTFH
                     SFSDRTEGRRIDWILHSPHLAPVAATIDRYRAADGRYPSDHYAVTATFRWNDTRPPTG
                     A"
     gene            1568..>2018
                     /locus_tag="VLI90_13330"
     CDS             1568..>2018
                     /locus_tag="VLI90_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15235.1"
                     /translation="MIRVLHLLGVDADFQTARTHQSLQRNLGDGFAIEKRSIGYRGDF
                     RNVPTAAIALRHEPAEIIHAWGVPAMLAAAMSGGARRILFSPNKFAGGKTVRWVRSAI
                     DYRNVQMLCATATQRRTAIEHGVPPARCHLIRPGVEFSRINRRRDDAL"
BASE COUNT          375 a          671 c          607 g          365 t
ORIGIN      
        1 gccttcgccg gttcatactt ctcgatcgtc cgcaccgccg gcggcacaac cgcggtagtc
       61 gatatgccat aggcgacact gcgatagcgc gcctgcgcgc ccgaccgacc gccgattacc
      121 aggcgaaatg agatctgccg cgtcgcgtcc ggaaaaaccg agcgatatgc cgccagcggc
      181 atctcgtata tcccgggctt gttgatcgcg cggaagatgt cgaacatctg aagattgttg
      241 cccgcgtcgt cccagcaaac gacctgcacc gacgtatcgc cgcccttgat gtcggctaca
      301 tcgactcgca ggacgccgcg gggatcgaac gggcaacgct cggtgagcat gacgtccgac
      361 cacggcgccc gactgccgag cgtgatcgca agatcagaac catcggccat aaccgtgaga
      421 ccgtcgctca ctttcacctg ggcgtgattg tcgtacaggt gcacgaggcg cacatccttg
      481 gcctgccatt gcaacgcgcc atcgtccgcg gcgacagcgc ggctgctcac cgccaggcat
      541 gtcagcattg cgatgcaagt acaaacaacc aaacgtcgat ttcgcatcat ggtcatgccc
      601 cggttggcgg tcgtgtgtcg ttccagcgga acgtcgcggt gacggcgtaa tgatcggagg
      661 gatatcgacc atcggccgcg cgatagcggt cgatcgtcgc cgccaccggc gccaggtgcg
      721 gcgaatgtaa tatccagtcg atccgccggc cttcggtccg gtcgctgaag ctgtggaagg
      781 tcgactcgtt cgccgacgcc tgcggatgca cgagccgata gctgtcgctc agctgaagat
      841 cgctcgacgt gtcacgcgac agcaacgccg cgtacggttc atccacctcg gtgctgttga
      901 aatctccggt gaggacgacc accccgccgc cggccaactc cggcagcact cggcggatca
      961 gcttcgcccc ttccgcgcgg gcgcgcgtgc ccatgtcatc cagatgcgtg ttggcgtgaa
     1021 gcagcgtcgc gccgttgagc cgatcgcgca cgcggaccca cgagcagatg cggacgtgtt
     1081 tggagtccca cgacttgctg ccggcgaccg ccggtgtctc agacagccag aagtttccca
     1141 cgtccagcag ttcgaatcgc tcaaggcgat agaggatcat ggcccattcg cccgatcgtt
     1201 tgccgtcatc gcgcgcgacg cccgcgaagc cgtagtcgcc caaatcctcg cgcagctcat
     1261 cgtgctgatc gacccaaacc tcctgcaggc cgagcagatc gggcgaaaag gcgcgcacgc
     1321 tggccttcca cacatcccgg cgatgaaccc aatggttcgg tccatccggt gcctgcgtgt
     1381 tccggatatt gtagctcatc acccgcaggt cattggcttg cgacaaatga acgtctcctt
     1441 tagaatctgt gcctccgcgc gctgcccatt ggaccatggg cggtggtgaa tttcaaccat
     1501 cagtgaaatg tttcaccgac gcgatggcct tgccgcaggt gacgtttcga ctacgcttat
     1561 cgcgcccatg atccgcgtcc ttcacttgct cggcgttgac gccgacttcc aaaccgcccg
     1621 cacgcatcaa agcctgcaac gaaatctggg cgacggcttc gcaattgaaa agcgatcgat
     1681 cggataccgc ggggatttcc gcaacgtccc aacggcggcg atcgcgctgc gacacgagcc
     1741 ggccgagatc attcatgcct ggggtgtgcc ggcgatgcta gccgccgcga tgagcggtgg
     1801 cgcccggcgg atcttgttca gcccgaacaa gttcgccggc ggcaaaacag tgcgctgggt
     1861 gcgatcggcc atcgattatc gcaacgtaca gatgctgtgc gcgacggcca cgcagcggcg
     1921 cacggccatc gagcacggcg tgccgccggc gcgatgtcac ctgatccgcc ccggtgtcga
     1981 gttctcgcgc atcaatcgcc ggcgcgacga tgcgctgc
//
LOCUS       DATIMH010000762         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1627618, whole genome shotgun sequence.
ACCESSION   DATIMH010000762 DATIMH010000000
VERSION     DATIMH010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627618"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VLI90_13335"
     CDS             complement(<1..590)
                     /locus_tag="VLI90_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15236.1"
                     /translation="MSLSTTAAKPSPASALLIAAPAVLIIVICCAMPLAWMLGAIVAN
                     PVVRTELKMTSFRAALLGRTIGYNGAAAIIATAMGLPAGIVLGRGRGWLAKILWLLLP
                     AALLMPSLSYAYGWSQFVRLTRPVFRPLGVTFIPGGVADTVRCIWSLAAWLWAVPAGM
                     VGLALRRLDTNVQQQAVLDGALYRVTFRQLIGPIVAS"
     gene            complement(684..1184)
                     /locus_tag="VLI90_13340"
     CDS             complement(684..1184)
                     /locus_tag="VLI90_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15237.1"
                     /translation="MGNTKAIMFTISTYGTWLRGDARGWVDDGIVFPAQPALQAWDRD
                     RMKHSPYLFPRDRWLEIGGAMGESLLKRFDARIRAWTLQGWHSHGVIGSTRHDIADII
                     KCVKDAARWHLRIIRPIWAADYDKRWCFEWPSVGNRVGYVEKHNVRNGCNPKPWPFIS
                     TPEELL"
     gene            complement(1225..>2004)
                     /locus_tag="VLI90_13345"
     CDS             complement(1225..>2004)
                     /locus_tag="VLI90_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15238.1"
                     /translation="ASAPRLSRMSLASTQSAPAAPLSLLRWSLPGRAIIFTLSAASIW
                     CLLSEFYGLCSMRTFTVWILIPASVALIAIAIYDRIAGDRRLWRSVLIGMAAGFVAAV
                     AYDIFRIPYVVAAATHAGPGWLRLPLYKVFPQFGAMILAQPFSPQQADSQFTLAAHVV
                     GWAYHLSNGITFGVMYVALLGDPREKSWWWGVVMAVGIEVMLLLTPYTGFFGIGLTAE
                     FVVATLAAHLIFGAVMGSTVRAWANIFAAPVRTTAPPFSPT"
BASE COUNT          424 a          677 c          603 g          300 t
ORIGIN      
        1 gacgcgacga tcggcccgat cagctgccgg aacgtcacgc gatacagcgc gccatcaagc
       61 accgcctgtt gctgtacgtt cgtatcgagc cgccgcagcg ccaggcccac catccccgcc
      121 ggcacggccc acaaccaggc ggcgagcgac cagatacatc gaacagtatc tgctactcca
      181 cccggaatga acgtgacacc gagcggccga aaaaccggcc gcgtgagccg gacgaattgc
      241 gaccagccat acgcgtagct gagcgacggc atcagcaacg ccgccggcag cagcagccag
      301 aggatttttg ccagccaccc gcgtccgcga ccgagcacga tccccgcggg caggcccatc
      361 gccgtcgcga tgatggcggc cgcgccgttg tacccgatcg tgcggccgag caacgccgcg
      421 cgaaagctgg tcatcttcag ctcggtccgc acgacgggat tcgccacgat cgccccgagc
      481 atccacgcca gcggcatcgc gcagcagatg acaatgatca acaccgccgg ggcggcaatg
      541 agaagggctg aagcaggaga aggtttggcg gcggtggttg aaagcgacac tgctatggaa
      601 gccttaggaa aagaacctgc cgcttgcgaa gtcgaacacc ccatttagcc cggcgtgaac
      661 acacgccggg tctgcgtcag atgtcaaagc agctcctccg gcgtcgaaat aaacggccac
      721 ggctttggat tgcacccatt gcgcacattg tgcttctcca catacccgac tcgattcccg
      781 acactcggcc actcgaagca ccaccgcttg tcataatccg ctgcccagat cggacgaatg
      841 atccgcagat gccacctcgc cgcatccttc acgcacttaa tgatgtccgc gatgtcgtgc
      901 cgcgtcgatc cgatcacgcc gtggctgtgc cagccctgca gcgtccaagc ccggatgcgc
      961 gcgtcaaatc ttttcagcag cgattcgccc attgctccgc cgatctcaag ccagcgatcg
     1021 cgcggaaaca ggtacggcga gtgcttcatg cgatcacggt cccacgcttg gagcgcgggc
     1081 tgcgcgggga agacgatgcc gtcatcaacc catcctcgtg cgtctccgcg cagccatgtg
     1141 ccgtacgtcg agatcgtgaa catgatggct ttcgtatttc ccatctgggc gtcctttacg
     1201 atctgacgta aacccggcgt gtgttcacgt cgggctaaat gggggcgcgg tcgtccgaac
     1261 cggcgcggcg aagatattcg cccacgcccg caccgtcgat cccatcaccg cgccaaagat
     1321 cagatgcgcc gccagcgtcg ccacgacgaa ttcggccgtc aagccgatgc cgaagaagcc
     1381 ggtgtacggc gtgagcagca gcatcacctc gatgccgacg gccatcacga cgccccacca
     1441 ccaggatttt tcgcggggat cgccgagcag cgcgacgtac atcacgccga acgtgatgcc
     1501 gttggacagg tgatacgccc agcccaccac gtgggcggcc agcgtgaact gcgaatcggc
     1561 ctgctgcggc gaaaacggct gcgcgaggat catcgcgcca aactgcggaa acaccttgta
     1621 caacggcaac cgcaaccatc ccggccccgc gtgcgtcgcc gcagcgacga cgtagggaat
     1681 gcgaaagatg tcgtaagcca cggctgcgac gaacccggcc gccatgccga tcagcacgct
     1741 gcgccacagt cggcgatcgc cggcgatgcg atcgtagatc gcgatcgcga tcagcgcgac
     1801 gctcgccggg atcaggatcc agaccgtgaa cgtgcgcatc gagcacaggc cgtagaactc
     1861 actcaacaaa caccagatgc tcgcggcgga gagtgtgaaa atgatggcgc ggcccgggag
     1921 gctccatcgc aacaacgaca gcggcgcggc cggcgcggat tgtgttgagg cgagcgacat
     1981 ccgtgatagc cggggggcgg aagc
//
LOCUS       DATIMH010000763         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4511716, whole genome shotgun sequence.
ACCESSION   DATIMH010000763 DATIMH010000000
VERSION     DATIMH010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4511716"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VLI90_13350"
     CDS             <1..1155
                     /locus_tag="VLI90_13350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSV15239.1"
                     /translation="PSSITLTGSGCTLLMVTRGPYFAPISFGTDGVWTINYGFLGGTP
                     ANIGIFNGGARAFPAVTYLPSLTPIVHGVRCSAPLSESRLYVGTNQQSVLNANWCNFD
                     MTGGAIGRSINFTVGGYGNFSFNGEIYEIAIFNFPLTDGQVKSVLAAMQTANQIRSDA
                     NSNQVIFVGDSLTAGGPGTAPAMSHCYPWQLCQQSGGAFKPLIVAAPGQNISQQQTAV
                     TNQVLPMDRTPFNVNAAVVCCGSNDIVQGRTDVQVIADLSAMCAGLRAAGYKTVVATI
                     TPRNAATGYTAGNITTLSSVNASIRSGYAAYADGLADWAADARLSDCTSATYFSDQCH
                     TTDAGDAVKAAIVKATLDPILLPPQVQLAYAAFYTPGRLYAGAFGSFLRS"
     gene            1646..2026
                     /locus_tag="VLI90_13355"
     CDS             1646..2026
                     /locus_tag="VLI90_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15240.1"
                     /translation="MPAPIPFQANNITVGTTLTKLIEIPLAGLDKDLSFELQNAGSAT
                     TNNFVIVRRLHDAGAWLNYLGGADFANATSKCVSSTPGPHALPTGQSAWVDVDCGAAV
                     AVQLWASVASGTAVLSVFGGARVR"
     gene            2253..2666
                     /locus_tag="VLI90_13360"
     CDS             2253..2666
                     /locus_tag="VLI90_13360"
                     /inference="COORDINATES: protein motif:HMM:NF018704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp36 family protein"
                     /protein_id="HSV15241.1"
                     /translation="MPTYAQQSDIEDLFGPANVAAWSRFETGTPSGPTDTSRVATALA
                     YADGEINAWFAGGPYALPLVCTLCKPLVTYWAAVIAGVWLYGNRASVSYIDYSGNRYI
                     ALRNAVYQDMDAYKAGVKRLDAAMRFPHATAPVGD"
BASE COUNT          594 a          983 c          960 g          561 t
ORIGIN      
        1 ccatcgtcga tcacgctgac cggcagcggg tgcacgttgc tgatggtcac gcgcggtccg
       61 tacttcgcgc cgatctcgtt cggcaccgat ggggtgtgga cgatcaacta cggtttcctc
      121 ggcggcacgc cggcgaacat cggcatcttc aacggtgggg cgcgcgcgtt ccccgccgtc
      181 acttatctgc cgtcgctcac gccgatcgtg catggcgtgc ggtgctcggc cccgctttcg
      241 gaaagccggc tgtacgtcgg caccaaccag cagtcggtgc tgaacgccaa ctggtgcaac
      301 ttcgacatga ccggcggcgc gatcggccgc tcgatcaact tcacggtcgg cgggtacggc
      361 aacttctcgt tcaacggcga gatctacgaa atcgccatct tcaatttccc gctgaccgac
      421 gggcaggtga agagcgtgct ggccgcgatg cagacggcga accagattcg cagcgacgcg
      481 aacagcaacc aggtgatctt cgtcggcgat tcgctgaccg ccggtggacc gggcacggcg
      541 ccggcgatgt cgcattgtta tccctggcag ctgtgccagc agtcgggcgg cgcgttcaaa
      601 ccactgatcg tcgccgcgcc ggggcagaac atctcgcagc agcagacggc cgtgacgaac
      661 caggtgctgc cgatggaccg cacgccgttc aacgtgaacg cggccgtcgt gtgctgcgga
      721 tcgaacgaca tcgtgcaggg ccgcaccgat gtgcaagtga ttgccgatct gtcggcgatg
      781 tgcgccggcc tgcgcgccgc gggatacaag acggtcgtcg cgacgatcac gcccaggaat
      841 gccgccacgg gatacaccgc cggcaacatc acgacgctca gcagcgtgaa cgcgagcatc
      901 cgcagcggct acgcggcgta cgccgatggt ctagccgatt gggccgccga cgcgcggctg
      961 tcggactgca cgagcgcaac gtacttcagc gaccaatgtc acacgacaga cgccggcgac
     1021 gccgtgaagg cggcgatcgt gaaggcgacg ctcgacccga tcctgctccc cccgcaagtg
     1081 cagctggcgt acgcggcgtt ctacacgccg gggcggttgt atgcgggggc gtttggaagc
     1141 tttttgcgat cttgaacgag acaaggggac acggagacaa gggtgattga tcatgtggac
     1201 gatggccgag atggcgtctt cgtatttgtc gttcagcttc ttgccgactt cgcgcgcgag
     1261 atagccgcag ctgacggcaa attcgagcca ggtctgcgtt tcaccggcct cggtctcggc
     1321 gcccgaaagc ttgctgatga atgcggcctg atagcggcgc tttcgccacg cctccgacag
     1381 atttgcattg accgatcgcg acgatcgacg cacctgatcc gtcagcgaat atcgctcctc
     1441 caacggaaaa cgatttggaa tggcgaaaga tcgccatcgc cgccgcatag gcgcgcttgt
     1501 agaccgtcaa ttcccgatgc gagttgatct tccggccatg tgatctccta aaccgccaga
     1561 tgaccaaccc cgtgtccccg tgtccccgtg tcgccttgtc ccccagtctc cccgtcccct
     1621 cgtctcttct aacacggagc cccccatgcc cgcacccatc ccattccagg cgaacaacat
     1681 caccgtcggc acgacgctca cgaagctgat cgagatcccg ctcgcggggc tcgacaagga
     1741 cctgagcttc gagctgcaga acgcggggtc ggcgacaacg aacaacttcg tcatcgtccg
     1801 ccggctgcac gatgcgggag cgtggctcaa ctatctcggc ggcgcggact tcgccaacgc
     1861 gacgagcaag tgcgtcagca gcacgccggg cccgcacgcg ctgcccaccg ggcagagcgc
     1921 gtgggtggac gtggactgcg gcgccgccgt cgcggtgcag ctgtgggcga gcgtggcgtc
     1981 gggcacggcg gtgctgtcgg tgtttggcgg ggcgcgcgtg cggtaacgcg acgcaaatcc
     2041 gaatgacgaa gtccgaatcc ccgaatcaat tccgaatggc gaatgacgaa actaggacga
     2101 agcacacaaa ccgcttcagc ggttagggtg ttcgttcgtc gaacacgtca aagtcgcgcg
     2161 tttcgtcatt cgaacattcg taattcgtca ttcattcggg gattcgggtt tcgccattcg
     2221 gatttcatcg aacgatcacc acggaggtcc ccatgcccac ctacgcccaa cagtccgaca
     2281 tcgaagacct cttcggcccc gcgaacgtcg cggcgtggtc gcggtttgaa accggcacgc
     2341 ccagcgggcc gacggatacg tcgcgcgtcg cgaccgcgct ggcttacgcc gatggcgaga
     2401 tcaacgcgtg gttcgccggc gggccgtacg cgctgccgct ggtgtgcacg ttgtgcaagc
     2461 cactcgtcac gtactgggcc gccgtcatcg cgggcgtctg gctgtacggc aaccgcgcca
     2521 gcgtcagcta catcgactac agcggcaacc gctacatcgc cctgcgcaac gccgtgtacc
     2581 aggacatgga cgcgtacaaa gccggcgtga aacgcctgga cgcggcgatg cggtttccac
     2641 atgcgaccgc gccggttggc gattgaacac gagcaaatcc cggggcgatg ggtgttttgt
     2701 agagttgtac gaccgacgct gtcgtccgat aaagtaagag ctgctctcgc ggcggagttt
     2761 taatcgacaa aaagccgacc tctacgttat attcgaagat tcagccaacg ttctatgctc
     2821 tatccgaaac agtgcgcagc atcatgccta aacctacacc ggttaagagg atcttagagg
     2881 tacgttacga gcggggttat cgctatctcg accacactgg cgaagccatg ctcattctac
     2941 aggaggtgct gccatccgag acaaacttgg tctggatgcc ggaggacatc gtacccgccg
     3001 gtgcgaagct gaaatgcccc gacctcgacc tccgaattgt attcgatgcg gcccacttcg
     3061 tggttgagca gagcggcgga gaagagacgg cgatgttt
//
LOCUS       DATIMH010000764         6896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1340866, whole genome shotgun sequence.
ACCESSION   DATIMH010000764 DATIMH010000000
VERSION     DATIMH010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340866"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VLI90_13365"
     CDS             complement(<1..298)
                     /locus_tag="VLI90_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15242.1"
                     /translation="MLDLGALVRYMGAHFNAANWTCPSDDITAHLPRFNVPAYPYSYT
                     INYLLDSTISRAPANGNNSRKWLLNSQTMKIARIRSNAEVCMALEESQATINDGV"
     gene            297..428
                     /locus_tag="VLI90_13370"
     CDS             297..428
                     /locus_tag="VLI90_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15243.1"
                     /translation="MRGLRNVCGGEYEAIFASPIALASGTTPITALNFADVSVQNVN"
     gene            complement(687..887)
                     /locus_tag="VLI90_13375"
     CDS             complement(687..887)
                     /locus_tag="VLI90_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15244.1"
                     /translation="MPSAKPLPVADGPVNMLFALFNSSTLLPAANISEPPLTTPDNVT
                     CVVYTFSAAPLPRLPLPLLMNG"
     gene            924..1454
                     /locus_tag="VLI90_13380"
     CDS             924..1454
                     /locus_tag="VLI90_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15245.1"
                     /translation="MGSAVSNLGYGGSGSIVNSGSVQFENYPLASSGDGTNFTTNSAI
                     SSTYHNISPISFGILNGVPSNNNYEPIQVSLAYDGLAHTLTESLLGLTTGGTFSFPYA
                     SVDFNTLLGGNSVTPGSPVAPVERRRCRRSATSSSAATTGRRRTSPVRSSRPRGGLRP
                     FSRASCPATTRPAWAG"
     gene            1418..2053
                     /locus_tag="VLI90_13385"
     CDS             1418..2053
                     /locus_tag="VLI90_13385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV15246.1"
                     /translation="MPGNNTTSVGGLTLNTGATVALTNIGSSGARGLLQTPTVTFAAP
                     SSKLDLGTNGLLVNESLSALRSQIFNSNIITTAAGLGIGSKDQGGGVVEAVVTLKGDA
                     NLDGTVDVGDLGALATNYGVSGGETWINGDFNYDNNVDVADLGALATNYNLSIGNGGA
                     GAAFGAGELASPTAAAVAASAASVPEPSALALIAAGAVGMLSRRRRRRLAR"
     gene            complement(2438..2911)
                     /locus_tag="VLI90_13390"
     CDS             complement(2438..2911)
                     /locus_tag="VLI90_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV15247.1"
                     /translation="MTVSSGADPLRAIMILIVDDFTDGAEVLCRLLGRRGYPCELAAS
                     GIEGLVKIRAHPPEQPLLVVLDEMMPGMGGVDVLRQIRSDPAISHTTVILYTAAFDEA
                     KRNTAITLGVAAWLLKGGTQGMGVDQVLDTITSWYERAGGVRAVADTSSEQNTNS"
     gene            complement(3171..4193)
                     /locus_tag="VLI90_13395"
     CDS             complement(3171..4193)
                     /locus_tag="VLI90_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSV15248.1"
                     /translation="MLNSDPMRITRRRMLGAIGAVALAGKSVQPLWAADATQPAGASQ
                     PGWSIWNSGPIVPPAQPGARISNKRFKVAADDEFLNNYRQDLREFAFAKTAGLDGVVL
                     DMGHMPGGKVLQNHLVDPAVREQFLAKSRETGVEIACLGFLAMYAWVVPHVPDIDAIT
                     HNWVDTMVQMGVKLGFMPLLTPDSTLAEPSHAAVVNETVALFKRVAPYAEKNGVILSI
                     ESNVNADGYKKFLDAVGSPAVRVFYDPGVGLENHFDVYQDIKDLGNDRVCALRLQQGS
                     VPPEKFEHRLGEGLIDFPRLRDTLMEMNWGGWMSIARSRLKGTERKAAPNMLANAAYV
                     HKMFPE"
     gene            4478..>6896
                     /locus_tag="VLI90_13400"
     CDS             4478..>6896
                     /locus_tag="VLI90_13400"
                     /inference="COORDINATES: protein motif:HMM:NF020844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016837 - carbon-oxygen lyase activity,
                     acting on polysaccharides [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase B N-terminal
                     domain-containing protein"
                     /protein_id="HSV15249.1"
                     /translation="MARLKQRRIPRRSRSRSSLIERLENRTLFAFGITSTTGSLASYV
                     IDNGADLKFSVLRPGATVTSTIHLGDVTSIKYKNQELLASYATTSRYSHYEQGLGSIA
                     TVTTTTGGTTGSRWILVTCDDSAETSGGVIQYYAVRENDNNLYMASLPTDVTNGPGEG
                     RFIAYLNRSVFTTPEAPSDNAGTTGAIEGSDVFGHADGTTTSKFYNMGRRMIDNTYHG
                     LTGTAGTTSVGAWMFMGNREHSAGGPFFKDIDYQSDAAVEIYNCLFTGHTQTEAFRQG
                     LQGPYAFQFTSGVAPTTPDYSWMSALNLQGWIPVAQRGAISGTASGMVAGHQTVVGLS
                     NGTAQYWTYANASTGSYSITGVQPGTYTETLYDNELEVGHKTVTITAGATSSANIVNT
                     FYIPASPIFRIGTWDGTPAGFLNADKISIMHPTDVRMSSWTATPNFVIGTNTDAQWPL
                     AQAIDVNNAQRITFTLTAAQVQSLSFRVGITWGFSGARPRITVNSGQTYAWTSTIPTA
                     SSDLNSRGITRGTWRGDNQIYTYSIPSSAVRAGVNTIDLPLVSGSTGAAWLSPNVAYD
                     AIELDTSSTASAPAIASVTVTPVNSSIGINGTRTFTAVARDANGNVIAANIDWSAARG
                     TIDANGNYTAPATLGSDTITATATTTRIAGYNSTSSSTSTISKTISGSGSTTINVLDA
                     VPSVATPASASPNPAFSLSTALSVLGSDDGGESNLTYTWSAINTPPGSVNFSINGTNA
                     AKNTTVTFGAYGSYDLLVTITDQQNNSVTSQVTVVCREHVARYKADETGGTTLADSSG
                     GNNTANLS"
BASE COUNT         1333 a         2240 c         2075 g         1248 t
ORIGIN      
        1 tcacgccgtc gttgatcgtt gcctgcgatt cttccagcgc catgcacacc tccgcattgc
       61 tgcggatgcg cgcgatcttc atcgtctgag agttcaacag ccatttccga ctgttgttcc
      121 cattggctgg ggccctggag atcgtgctgt ccagcaggta gttgatcgtg tagctgtagg
      181 gataagcggg aacgttgaac ctgggcaggt gcgcagtgat gtcatcggag gggcacgtcc
      241 aattggcggc attgaaatgt gcgcccatat agcgcaccag cgcgccgagg tcgagcatgc
      301 gcgggttgag aaacgtgtgc ggcggagaat atgaggcgat cttcgccagc ccgattgcgc
      361 tggccagcgg caccacgccg atcaccgcgc tgaacttcgc cgatgtgtca gtgcagaacg
      421 tcaattgacc ggcgtgaatt tccggtccgg gcgcgctgaa cctgaccggt cacctcggta
      481 tcgccggcac caacacctac acgggcgggg cgcccgtcaa tggcagcacc gacattgcca
      541 ccgacaatcc cagcgtcttt cggcaccggg acgatcacgt tcaaccgcgg cgggatcggc
      601 accctgccgg acacgccggc gaccggcctc acgatttcga acaactacaa cttcggcgcc
      661 gatccgtccg gatatttcga gaactatcac ccgttcatca agagcggcaa cggcaacctg
      721 ggcagcggcg cagcgctgaa cgtgtacacc acacaggtga cgttgtcagg cgtggtcagc
      781 ggtggttcgc tgatgttggc cgcgggcagc aacgtgctgg agttgaacaa tgcgaacaac
      841 atgttcacgg gcccgtccgc caccggcaat ggtttggccg acggcatttc acggttcaaa
      901 aagatccacg cggcgccagc gcggtgggct cggccgtttc caatctcgga tacggcggca
      961 gcggctcgat cgtcaacagc ggctcggttc aattcgagaa ctatccgctg gccagcagcg
     1021 gagacggcac gaatttcacc accaatagcg cgatctcctc gacgtaccac aacatctcgc
     1081 cgatctcgtt cggcatcctg aacggcgtgc cgtcgaacaa caactacgag ccgatccagg
     1141 tgagcctcgc gtacgatgga ctcgcccaca cattgaccga atcgctgctg ggcctgacca
     1201 ccggcgggac gttctcgttc ccatacgcca gcgtggactt caacacgctg ctcggcggga
     1261 attccgttac gccgggttca ccggtggcac cggtggagcg gcggcgctgc agacgatcag
     1321 caacttcgtc ttcagcagct acgacgggac gccgcagaac atctccagtt cgatcgtcgc
     1381 ggccccgggg aggtcttcgg ccattcagcc gagcgtcgtg cccggcaaca acacgaccag
     1441 cgtgggcggg ttgaccctga acaccggcgc gacggtggcg ctgacgaaca tcggttcgag
     1501 cggcgctcgc gggctgctgc agacgccgac ggtgaccttc gccgcgccgt cgtcgaagct
     1561 ggacctgggg accaacggac tactggtcaa cgagagcctg tcggccctgc gcagccagat
     1621 cttcaacagc aacatcatca cgacggccgc ggggctgggg atcggttcga aggatcaagg
     1681 cggtggggtc gtcgaagccg tcgtcacgct caagggcgat gcgaacctcg acggcacggt
     1741 cgacgtgggc gacctgggcg cgctggcgac gaactacggc gtcagcggcg gtgagacctg
     1801 gatcaacggt gactttaact acgacaacaa cgtggacgtg gccgacctgg gggcgctggc
     1861 gacgaattac aacctgagca tcggcaacgg cggcgccggt gctgcctttg gggcaggcga
     1921 actggcatcc cccaccgccg ccgccgtcgc cgccagcgcg gcgtcggtac cggagccgag
     1981 cgcgcttgcg ctcatcgcag ccggcgcggt tggcatgctg tcgcgccgcc gccgccgccg
     2041 gttggctcgt tgaagttgac aatcaaagac aggtgagatg tcgaaccgca ggcgctggag
     2101 ctgaccctcc gcgcctgcga tttttgtttg cgcttaggaa gcgctggcgc gttatcgtga
     2161 attgcggata agagtcgttc caaaccttaa tcgtgatagg ggcctccggc ccgtgcttgg
     2221 tgcttgggta gtcgtagagc ggtgattctc tccaaatcaa gcgcatcgcg cacgggccgg
     2281 aggcctgtgt cacgcggtga ggcgttcgaa gttctaaaac caatctcacc gtctgtttgt
     2341 ctcgaagaaa cgaacacacg ctaagtcgca agcggcggaa tgaatcggcg cagtcgatga
     2401 ctcgtcagca tattcgacgt cgaatccttc gactggttca cgagttcgtg ttttgctcgg
     2461 atgaagtgtc agcaaccgcc cgcacgccgc cggcgcgttc gtaccagctg gtgatcgtat
     2521 cgagcacctg atcgacgccc atgccctggg tgccgccctt gagcaaccac gcggcgacgc
     2581 cgagcgtgat ggcggtgttg cgctttgctt cgtcgaaggc ggcggtgtag aggatgacag
     2641 tggtgtgcga gatggccgga tcgctgcgga tctggcggag cacgtcaacg ccgcccatgc
     2701 ccggcatcat ctcgtcgagc acgacgagca gcggctgctc gggcgggtgc gcgcggatct
     2761 tgaccaggcc ctcgatgcca ctggcggcca gctcacacgg ataaccgcgg cggcccagca
     2821 gccggcagag cacctcggct ccgtctgtga agtcatccac gatgaggatc atgatcgcac
     2881 gtaatggatc agccccgctt gaaaccgtca acggggaaaa tttcctgtcg aagatttgct
     2941 gccgatgcac ggccgttccc gggcgaattg gtctcgcgaa gccgcagaca cccggggtgc
     3001 ggcgcttgat gttaatagag attcttcgag ccaagggcca agagacgcaa gactaccgct
     3061 ttgcggtccg gttctcctgc cttcgcgtct gtcttcgcgt ctgcgcgtct tcgcggtcgc
     3121 gtttctttct gcaaattcgg atgaaacatg cgcagtttcc gctccatcga tcactccgga
     3181 aacatcttgt gcacgtaagc cgcgttcgcc agcatgttgg gcgcggcctt tcgttcggtg
     3241 cctttgaggc gcgagcgcgc gatgctcatc cacccgcccc agttcatttc catcagcgtg
     3301 tcgcgcagcc tggggaagtc gatcagaccc tcacccagtc gatgctcaaa cttttccggc
     3361 ggcaccgatc cttgttgcag ccgcagcgcg cacacgcggt cgttgccgag gtccttgatg
     3421 tcctgataga catcgaaatg attttcgagg cccacgccgg gatcgtagaa cacacgaacg
     3481 gctggtgaac ccacggcatc caaaaacttc ttgtacccat cggcgttgac gttcgactcg
     3541 atgctcaaga tgacgccgtt tttctcggcg tagggcgcga cgcgcttgaa cagcgcgacc
     3601 gtctcgttca ccaccgccgc gtgcgacggc tcggccagcg tgctgtccgg ggtcagcagc
     3661 ggcatgaagc ccagcttcac gcccatctgc accatcgtgt cgacccagtt gtgcgtgatc
     3721 gcgtcgatgt ccggcacgtg cggcaccacc cacgcgtaca tcgccagaaa tccaagacag
     3781 gcgatctcga cgccggtctc gcgcgacttg gccaaaaact gttcgcgcac cgccgggtcc
     3841 accagatggt tttgcagcac ctttccgccg ggcatgtggc ccatgtccag cacgacgccg
     3901 tcgagccccg cggtcttggc gaaggcgaac tcccgcaagt cctggcgata attgttcagg
     3961 aattcatcgt cggccgcgac tttgaaacgc ttgtttgaaa ttcgagcccc cggctgagcc
     4021 ggcggcacga tggggccgga gttccatatt gaccaacccg gctgactcgc ccccgccggt
     4081 tgcgttgcgt ccgccgccca aagcggctgc accgatttac ctgcgagtgc caccgcgccg
     4141 atggccccca acatccgccg ccgagtgatc cgcatggggt ctgaattgag catgaagcgc
     4201 ctcccgatgg ttcgatatta cccctgtcgg acgcggttca acagcggtgg tactaccacg
     4261 ctcatgtttt gtgccccgcg aggaaagaag gcgtccagac cccctcaaac cggatgaatg
     4321 acacgtgttg tgctaaactt ccactatgcg aaataattat tgaattttgc agaaactttg
     4381 tggtagtcta tggcgtactt cgtgtgaatt gccttgttgt ccggtgcacg cccgcggcgg
     4441 agtccttttt cgtacaaccc gttccagagg tcgcccgatg gctcgtctga agcagcgtcg
     4501 aatcccacga cggtcccgct ctcggtcctc gctcatcgag cgcctcgaaa accgcactct
     4561 cttcgcgttc ggcatcacca gcaccaccgg ttcgctggcc agctatgtca tcgacaacgg
     4621 cgctgatctg aagttcagcg tgctgcgccc cggcgcaacg gtcaccagca ccatccacct
     4681 tggcgacgtt acgtcgatca aatacaagaa tcaggagctt ctcgcctcgt acgccaccac
     4741 cagccgctac tcgcactacg agcagggtct cggctcaatc gccaccgtca ccacgaccac
     4801 cggcggcacc accgggtcgc gctggatcct ggtcacctgc gatgactcgg ctgagaccag
     4861 cggcggtgtc attcagtact acgccgtccg cgagaacgac aacaaccttt acatggcgtc
     4921 gctgccgacc gacgtgacca acggcccggg cgaaggccgg ttcatcgcgt acctcaaccg
     4981 ctccgtcttc acgacgcccg aagcgccgtc ggacaacgcg ggcaccaccg gcgccatcga
     5041 aggctccgac gtcttcggcc atgccgacgg caccaccacc tccaagtttt acaacatggg
     5101 ccggcggatg atcgacaaca cgtaccacgg cctgaccggc accgccggga ccacttccgt
     5161 gggcgcgtgg atgttcatgg gaaaccgcga gcactcggcc ggtgggccgt tcttcaagga
     5221 catcgattac cagtccgacg ccgccgtcga aatttacaac tgcctgttca ccggccacac
     5281 gcagaccgaa gcgtttcgcc agggactgca ggggccttac gcgtttcagt tcacgagcgg
     5341 cgtcgcgccg accacgccgg attattcgtg gatgtcggcg ttgaacctgc aaggctggat
     5401 tcccgtcgct cagcgcggcg cgatcagcgg gaccgccagc gggatggtcg cgggtcatca
     5461 aaccgtcgtc ggcttgagca acggcaccgc gcaatactgg acctacgcca acgccagcac
     5521 tggaagctac agcatcaccg gcgttcaacc cggcacgtac accgagacgc tttacgacaa
     5581 cgaacttgaa gtcggtcaca agaccgtcac gatcaccgcc ggcgcaacga gcagcgccaa
     5641 catcgtcaac acgttctaca tcccggcgag tccgatcttc cgcatcggca cgtgggacgg
     5701 cacgcccgcg ggatttctta atgccgacaa gatctcgatc atgcacccga cggatgtgcg
     5761 gatgagcagt tggaccgcga cgccgaattt tgtgatcggc accaacaccg atgcgcagtg
     5821 gccgctggct caggcgatcg acgtgaacaa cgcgcagcgc atcacgttca cgctgacggc
     5881 cgcgcaagtg cagagcttat ccttccgcgt cggcatcacg tgggggttct ccggcgcgcg
     5941 tccgcggatc accgtcaact ccggtcagac ttatgcctgg acgagcacga tcccgacggc
     6001 cagcagcgac ttgaacagcc gcggcatcac ccgcgggacg tggcgcggcg acaaccagat
     6061 ctacacctac agcattccca gcagcgccgt tcgtgccggt gtgaacacga tcgacctgcc
     6121 gctggtcagc ggcagcaccg gcgccgcttg gctgagcccc aacgtcgctt acgacgcgat
     6181 cgaactggac accagcagca cggcgtcagc gccggcgatt gccagcgtga cggtcacgcc
     6241 ggtgaattcg tcaatcggca tcaacggcac gcgcaccttc accgcggtgg ccagggatgc
     6301 gaatggaaat gtgattgccg caaatattga ctggtccgcc gcccgcggga cgatcgacgc
     6361 caacggcaac tacaccgcgc cagccacgct tggatcggac acgatcactg cgacggcgac
     6421 gacgacgcgg atcgccgggt acaactcgac gtcgtcctcc acctcgacga tcagcaaaac
     6481 gatttccggc agcggcagca cgacgattaa cgtgctcgac gcggtgccga gcgtcgccac
     6541 gccggccagc gcatcgccca atccggcgtt ttcgttgtca accgcgctca gcgtgcttgg
     6601 cagcgatgac ggtggcgagt cgaatctgac ctacacgtgg tcggccatca acacgccgcc
     6661 tgggtcggtc aacttctcca tcaacggcac gaacgcggcg aagaacacca ccgtcacgtt
     6721 cggcgcgtat ggttcgtatg acttgctggt gacgatcacg gatcagcaga ataacagcgt
     6781 cacgagccag gtgacggtcg tgtgccgcga gcacgttgca cgctacaagg ccgacgaaac
     6841 cggcggcacg acgctggccg attcgtccgg tggaaacaac acggcgaacc tgagcg
//
LOCUS       DATIMH010000765         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5094711, whole genome shotgun sequence.
ACCESSION   DATIMH010000765 DATIMH010000000
VERSION     DATIMH010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5094711"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(748..960)
                     /locus_tag="VLI90_13405"
     CDS             complement(748..960)
                     /locus_tag="VLI90_13405"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HSV15250.1"
                     /translation="MAQWPSTRAARVLAALLRTGWSIKRQSGSHRTLQRPGSPDVIFS
                     FHDREEIGPRMLVRIAKQTGLRPEDL"
     gene            complement(963..1169)
                     /locus_tag="VLI90_13410"
     CDS             complement(963..1169)
                     /locus_tag="VLI90_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSV15251.1"
                     /translation="MMYRVETEQEVDGRWIAEVPELPGVMAYGVSRDEAVRKAQALSL
                     RVLAERLEHGEALPQVDNVFSIVA"
     gene            complement(1238..>2522)
                     /locus_tag="VLI90_13415"
     CDS             complement(1238..>2522)
                     /locus_tag="VLI90_13415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HSV15252.1"
                     /translation="MNRVVITGIGWVTPMGHSIESVWRRLLNGESGIARTTLFDASTF
                     PTSISAEVKDFDLTDHVPQAAEHAGAGRNTAFALAACAQAWKSAGLDAASLDLDRVGT
                     YLAGGEGELDFDAFTAAALTAWDAETNTVDTVKWAEIARERMTATRELEQEPNMPLSH
                     LALLTGARGPALNCLTACAASTQAIGEATQILRRGDADVMIGGGTHSMIHPFGVTGFN
                     RLTALSTANENPPRASRPFDAQRGGFVLGEGAGMVILETLEHAQRRGATILAEVVGYG
                     STADAFRITDQDPEGAGAIGAMQAALADAKLSPKDIQYVNAHGTGTRENDGNETLAIK
                     KVFADNAKACPVSSIKSMMGHLIAAAGAVELICCVLAIRDNVLPPTMNLDNHDPECDL
                     DYVPNKARKARVDAALSNSFGFGGQNDSIIVKRFG"
BASE COUNT          447 a          843 c          759 g          473 t
ORIGIN      
        1 cgacatgctg gcaacgcttc gccgcgtctt gtggacacag cgaataaaag gcaactcggc
       61 ccttgggggc cgagtgcggc gattacttga aactctacgc ttcacgctct gcgccgccgc
      121 gtgaaagagc taaaccgtac aattctcgaa agcccctttt agcggcggcc cgtagggccg
      181 agtgcgcccg ccattaagat agacccaatc atcggctaca ccagttaaca ctgcgctaag
      241 aatgaccgcg caacagcgtg gcacggggag tcggggcaaa agaaagggga actgccgccc
      301 gcgcgcaaga tgcacgccgg cggcagttcc cggttgttta cgaacttaaa ttagcgacta
      361 ctcgcgatcc tcagcccagc gaatcaacgc ttgcggcggt cgcggcgagc cttgcgcttc
      421 ttgctgccga tcttacgtcg gcggcgcggc ttagccatgc ggcgtctcct ttccaaacta
      481 gaacgcggca cagggtaacg ggtcgatcga atcgcgtcta cccaacggcg gcggtgaaag
      541 atcgggaggg tacgactttg atttgtggct cgattctggt ccgtccccct cgcccggtac
      601 tgctgaccgc aggtctccgg gagagggtcg gggtgagggt gacgccgagc atccgcgcga
      661 tcttcgcctg cgactgccgc cctttggaaa gatggcggac gatgtccgcc gaaagcgtgg
      721 tcgagaccgt cctgcgattg cccgacatca aaggtcctcc ggcctcaagc ccgtttgctt
      781 cgcgatcctt acgagcatcc gcgggccgat ctcttcacga tcgtgaaatg aaaaaatcac
      841 atcaggcgag cctgggcgct gcaacgttcg atgcgacccg ctttggcgtt tgatcgacca
      901 gccagttcgc aacaacgccg ccaaaactcg cgcggcacgt gtcgacggcc actgcgccat
      961 aactaagcca cgatggaaaa gacgttatcg acctgcggta gcgcctcgcc atgctcgagc
     1021 cgctccgcaa gcacgcgaag cgacagcgcc tgcgcttttc gcaccgcttc atcgcgcgac
     1081 acgccgtacg ccatcacacc gggaagttca ggcacttcgg cgatccaccg accgtccact
     1141 tcctgttcgg tttcgactcg gtacatcatt ctattctacc atgtgcagcc agacgttcag
     1201 aagtgaaatc agcttttcgc gcaactgccg ctcaccttca cccaaaccgc ttcacaataa
     1261 tcgaatcatt ctgccccccg aatccaaagc tgttcgacaa cgccgcgtcc acccgcgctt
     1321 tcctcgcctt gttcggcacg taatccagat cgcactccgg atcgtgattg tccaggttca
     1381 tcgtcggcgg cagcacgttg tcgcggatgg ccagcacgca gcagatcagc tccaccgcgc
     1441 cggcggcggc gatcaggtgg cccatcatgc tcttgatgct gctgaccggg cacgccttgg
     1501 cgttgtcggc gaagaccttc ttgatcgcca gcgtttcgtt cccgtcgttc tcgcgcgtgc
     1561 cggtgccgtg ggcattcacg tactggatat ccttcggcga gagcttggca tcggccagcg
     1621 cggcttgcat cgcgccgatc gcgccagcgc cttcgggatc ctgatcggtg atgcggaacg
     1681 cgtcggcggt cgagccgtag ccgacgactt ccgccagaat cgtcgcgccg cgccgctggg
     1741 cgtgttcgag cgtttccagg atgaccatgc ccgcgccttc gccgagcacg aacccgccgc
     1801 gctgggcgtc gaacggccgg ctggcgcgcg gcggattttc gttcgccgtc gatagcgccg
     1861 tcaggcggtt gaacccggtc acgccgaagg gatggatcat gctgtgcgtc ccgccgccga
     1921 tcatgacgtc ggcatccccg cggcggagga tttgcgtggc ctcaccgatc gcctgcgtcg
     1981 aagccgcgca cgcggtgaga cagttcagcg ccggaccacg cgcgccggtc agcagcgcca
     2041 gatgcgacag cggcatgttg ggctcctgct ccaattcgcg cgtggccgtc atgcgctcgc
     2101 gcgcgatctc agcccatttg accgtgtcga cggtgttcgt ctccgcgtcc cacgccgtga
     2161 gcgccgcggc ggtgaaggcg tcgaagtcca gttcaccttc accaccggcg aggtacgtgc
     2221 caacgcgatc cagatcgagc gacgccgcgt ccagccccgc ggacttccac gcctgcgcgc
     2281 acgccgccaa tgcgaatgcg gtgttgcggc ccgcccccgc gtgctccgcc gcctgcggca
     2341 cgtggtcggt cagatcgaaa tctttgacct ccgcggagat cgacgtgggg aatgtggacg
     2401 cgtcgaatag cgtggtgcgc gcgatgccgg attcgccgtt gagcaaccgc cgccagacgc
     2461 tctcgatgga atgccccatc ggcgtgaccc aacctattcc ggtgatgacg actcggttca
     2521 tg
//
LOCUS       DATIMH010000766         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4223483, whole genome shotgun sequence.
ACCESSION   DATIMH010000766 DATIMH010000000
VERSION     DATIMH010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4223483"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2451
                     /gene="rpoC"
                     /locus_tag="VLI90_13420"
     CDS             <1..>2451
                     /gene="rpoC"
                     /locus_tag="VLI90_13420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSV15253.1"
                     /translation="PVIPPDLRPLVLLESGNFATSDLNDLYRRIINRNNRLKKLVDLN
                     APEVIIRNEKRMLQQAVDALFDNGRCRRPVLGSSNRPLKSLTDMIKGKQGRFRENLLG
                     KRVDYSARSVIVVGPELHLHQCGLPKKIALELYQPFIIRKLKEHGLADTIKSAKKMIE
                     RRDKEIWDILEEVIKGHPVLLNRAPTLHRMGIQAFEPVLIEGNAIKIHPLVCKGFNAD
                     FDGDQMAVHLPLSVEAQAEAHILMLSTHNIFSPASGSPIIGPSQDIVLGIYYLTVAHK
                     GEPGHRETGKELAFRDVHEALLAYNRKQIGMHTMIKVRVDRDHVTTNDSKPETPIPAN
                     KVLTTTVGRLIFNDILPVGMPFYNLALSAKGGSRVIADCYKFLGRPATIDLLDNMKEL
                     GFKNSTLAGLSFAITDLRIPAKKQTIIDSQQRKADRVEKNLSIGAITERERYNALLEI
                     WSHARNLVTDEMMDELKNDTRDAGGGPLTPNDPKGIPYLNPVALMADSKARGSVDNFR
                     QLSGMRGLMSRPSGEIMETPIKTNFREGLSVLEYFTSTHGARKGLADTALKTADSGYL
                     TRKLADVAQNVIVGRHDCGTQNGIRKSAIYKGEVVDVSLRDAIVGRCSSDTIINPITD
                     EVIVREGQIISETIAGKIESLNIETVRVRSPLTCEAPTGVCAKCYGVDLSTGKLVEEG
                     LAVGIIAAQSIGEPGTQLTMRTFHTGGIGNVTEMKSEVRVVTTGKIEFRDLNAVEVTD
                     AEGGKHMVVLKRNGYLAIVDAKARELERQPVPYGATLLVKEKDVVKGGQQIAVWDPHR
                     IPILAEKGGRIEFQDIEE"
BASE COUNT          532 a          789 c          718 g          412 t
ORIGIN      
        1 tccccgtgat cccgcccgat ctgcggccgc tcgtgctgct cgaatcgggc aacttcgcca
       61 ccagcgattt gaacgacctc taccgccgca tcattaaccg caacaaccgg ttgaagaagc
      121 tggtggattt gaacgcgcca gaagtcatca tccgcaacga aaagcgcatg ctccagcagg
      181 cggtcgatgc gttgtttgac aacggccgct gccgccgccc cgtgttgggt tccagcaatc
      241 gcccgctgaa gtctttgacc gacatgatca agggcaagca gggccggttc cgcgagaacc
      301 tgctcggcaa gcgcgtcgac tactcggccc gttcggtcat cgtggtcggc cctgagctgc
      361 atctgcacca gtgcggcctg ccgaagaaga tcgcgctcga actttaccag ccgttcatca
      421 tccgcaagct caaagagcac ggcctggcgg acacaatcaa gtccgccaag aagatgatcg
      481 aacgccgcga caaggaaatt tgggacattc ttgaagaagt catcaagggc cacccggtgc
      541 tgctgaatcg cgctccgacg ctgcaccgca tgggcatcca ggcgtttgag ccggtgctca
      601 tcgaaggcaa cgccatcaag attcacccgc tggtctgtaa gggcttcaac gccgactttg
      661 acggcgacca gatggccgtg catttgccgc tgtcggtgga agcacaggcc gaggcacata
      721 tcttgatgct ttcgacgcac aacatcttct cgccggcgtc gggcagcccg atcatcgggc
      781 cctcgcagga catcgtgctt ggcatctact atctgaccgt ggcccacaag ggcgagccgg
      841 gccaccgcga gacgggcaag gaactggcct tccgcgacgt gcacgaggcg ctgttggcct
      901 acaaccgcaa gcagatcggc atgcacacca tgatcaaggt gcgcgtcgac cgcgaccatg
      961 tgaccaccaa cgactcgaag cccgaaacgc ccatcccggc gaacaaggtg ttgaccacca
     1021 ccgtcggccg cttgattttc aacgacatcc tccccgtcgg catgccgttc tataacctgg
     1081 ccctttcggc caagggtggc agccgcgtga tcgcggattg ctacaaattc ctcggccgtc
     1141 cggcgaccat tgacctgctg gacaacatga aggaactggg cttcaagaac tcaacgctgg
     1201 ccggtttgtc cttcgcgatc accgacctgc gtattcccgc gaagaagcag acgatcattg
     1261 actcgcagca gcgcaaggcc gaccgcgtcg agaagaacct ctccatcggc gccatcaccg
     1321 agcgcgaacg ctacaacgcg ctgctggaaa tctggtcgca cgcccgtaac cttgtgaccg
     1381 acgaaatgat ggacgaactg aaaaacgaca cgcgcgacgc cggcggcggt ccgctgacgc
     1441 cgaatgaccc caagggcatt ccgtatctga atcccgtcgc cctgatggcc gactccaagg
     1501 cccgcggcag cgtggataac ttccggcagc tctcgggtat gcgtggtttg atgtcccggc
     1561 cgtcgggcga aatcatggaa acgcccatca agaccaactt ccgcgaaggc ctctccgtgc
     1621 tggaatattt caccagcacg cacggcgcgc gtaagggtct ggccgatacg gctctgaaaa
     1681 ccgccgactc gggctacctc acccgtaagc tcgccgacgt ggcgcagaat gtcatcgtgg
     1741 gccggcatga ctgcggcacg cagaacggca tccgcaagtc agccatctat aagggtgaag
     1801 tggtcgatgt gagtttgcgc gacgcgatcg tcggccgctg ctccagcgac acgatcatca
     1861 acccgatcac cgatgaagtg atcgtccgcg aagggcagat catcagcgaa accatcgccg
     1921 gaaagatcga atcgttgaac atcgaaaccg tccgcgtccg ctcgccgctg acctgcgaag
     1981 cgcccaccgg cgtgtgcgcc aagtgctacg gcgttgacct ttccaccggc aagctggtgg
     2041 aagaaggcct ggccgtgggc atcatcgccg cacagtccat cggtgaaccg ggcacgcagc
     2101 tcacgatgcg tacgttccac accggcggca tcggcaacgt gaccgaaatg aagagcgaag
     2161 tgcgcgtggt caccacgggc aagattgaat tccgcgacct caacgcggtc gaagtgaccg
     2221 acgccgaggg cggcaagcac atggtcgtcc tgaagcgcaa cggctacctg gccatcgtcg
     2281 acgccaaggc ccgcgagctc gaacgccagc cggtgcccta tggcgccacg ctgctcgtga
     2341 aagaaaagga cgtggtcaag ggcggccagc agatcgcggt ctgggatccg caccgcattc
     2401 cgattctcgc ggaaaagggc ggccggattg agttccaaga catcgaggaa g
//
LOCUS       DATIMH010000767         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_470952, whole genome shotgun sequence.
ACCESSION   DATIMH010000767 DATIMH010000000
VERSION     DATIMH010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470952"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VLI90_13425"
     CDS             <1..519
                     /locus_tag="VLI90_13425"
                     /inference="COORDINATES: protein motif:HMM:NF023549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HSV15254.1"
                     /translation="VTVRTNIKSLVGGATEETIVARVGEGIVNAIGSAEDYKHVLGNP
                     DTISKAVLNKGLDANTAFEILSIDIAEITVGENVGAKLAADRAKSDTQRFQAEAEQRR
                     AMAVAHEQENVAKQAENRAAVILAEAEIPRAMADAFRSGNLGVMDYYKLKNIQADTGM
                     REAIAKPGDGKA"
     gene            581..1030
                     /locus_tag="VLI90_13430"
     CDS             581..1030
                     /locus_tag="VLI90_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15255.1"
                     /translation="MDELIILIIKALAKAFGGDQPRRSGKGPPLPPMRGIIPPTPEQI
                     RQQQILRATGGRRRKQAGVRAVPARVAAASAPDVLEVVEVLPDAPPTSARAAQRAAPS
                     NCAVTAHTGGSSVSAVALRKWLTPAVLQKQFILTEILQPPLALREEK"
     gene            complement(1093..>2421)
                     /gene="mnmG"
                     /locus_tag="VLI90_13435"
     CDS             complement(1093..>2421)
                     /gene="mnmG"
                     /locus_tag="VLI90_13435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HSV15256.1"
                     /translation="GCLAKLGLELGRLKTGTPPRLRRQSIDFAAFELQPGDEAPVPFS
                     YLNEYEDEAHGWRSVGFRGWTPPLMQVPCYLSGTNERIHEIIRANLHRAPMYSGQIQS
                     TGPRYCPSIEDKVVRFADKTRHQIFLEPEGLDTDEIYCNGISTSLPADVQEQIVRLVP
                     GLERAEILRHGYAVEYDMVWPTQIRSTLETKRIAGLFLAGQINGTSGYEEAAAQGIIA
                     GINAARYAACGLADFVLRRDQAYIGVLIDDLVTKPPTEPYRMFTSRAEHRLHLRNDNA
                     DERLTPIGRGVGLVDDDRWRRFESRRGEIEIALELLRASRVDDIAAIDYLRRSDTTWS
                     SIPALAGIAPDVGRQVEIRVKYEGYIARQDRQIERFAKMETKLIPPTLDYSRVAGLRN
                     EARQKLTKFTPRSLGQALRISGITPADVTLLAVHLDRRGRMQNDECRMQN"
BASE COUNT          458 a          798 c          739 g          426 t
ORIGIN      
        1 gtcaccgtcc gcaccaacat caagagcctg gtcggcgggg ccaccgagga gacgatcgtc
       61 gcgcgcgtcg gtgaaggcat cgtcaacgcg atcggatcgg ccgaggatta caagcacgtg
      121 ctgggcaatc ccgacacgat ctcaaaggcg gtgctgaaca aggggctcga tgccaatacg
      181 gcgttcgaga tcctctcgat cgacatcgcg gaaatcacgg tcggcgaaaa cgtcggtgcg
      241 aagctggccg ccgatcgcgc caagtcggac acgcagcgat tccaggccga agcggaacag
      301 cgccgagcga tggcggtggc gcacgagcag gagaacgtcg ccaagcaggc ggagaaccga
      361 gccgcggtca tcctggccga agcggaaatc ccgcgcgcga tggctgatgc gttccgcagc
      421 gggaacctgg gcgtgatgga ttactacaag ctgaagaaca tccaagccga cacagggatg
      481 agagaagcga tcgccaagcc cggcgatgga aaggcgtgac ccagccgctt gcggcttagc
      541 gtctgttctt cctcaataag gcaaggcgtc ggagcgggta atggacgaac tgatcatcct
      601 gatcatcaaa gcgcttgcca aagcgttcgg tggcgatcaa ccgagaaggt ccggcaaggg
      661 gcccccgctg ccgccgatgc gcggcatcat cccgccaacg cccgagcaaa ttcgccaaca
      721 gcaaatcctg cgcgccaccg gcggccgtcg ccgcaagcaa gcgggcgttc gcgccgtccc
      781 agcgcgcgtc gctgccgcga gcgcgcccga tgtgctggaa gtggtggaag tattgcccga
      841 cgccccgccg acaagcgcgc gggcggccca gcgcgcggcc ccgtcgaact gcgccgtcac
      901 ggcgcacact ggcggttcat ccgtcagcgc ggtcgcgcta cgcaaatggc tcaccccggc
      961 ggtgctgcag aagcagttca tcctgaccga aatcctgcaa ccgccgctgg cgctgcggga
     1021 ggagaaatga aacgcgcagt ttcgaccacg ccgggttcgg aggtactccg acgacctgga
     1081 ttggtgcgat cttcaattct gcattctgca ttcatcattc tgcattcgac ctcgccgatc
     1141 cagatgaacc gccagcagcg tcacgtccgc cggcgtgatg ccactgatgc gcagcgcctg
     1201 ccccagtgat cttggtgtga acttcgtcag cttctgccgc gcttcgtttc gcagtcccgc
     1261 cacccgcgag taatccagcg tcggcgggat cagcttcgtt tccatcttcg cgaaccgctc
     1321 gatctgccgg tcctgccggg cgatgtagcc ttcgtatttc acgcggattt ctacctgccg
     1381 cccgacatcc ggtgcgatgc cggccagcgc tgggatgctc gaccacgtcg tgtccgatcg
     1441 ccgcaggtaa tcgatcgcgg cgatgtcgtc cacacggctc gcgcgaagca actcgagcgc
     1501 gatttcgatc tcgccgcggc gcgattcaaa ccgccgccat cgatcgtcat cgaccaatcc
     1561 cactccgcgc ccgatcggcg tcaatcgctc gtcggcgttg tcgttccgca ggtgcagccg
     1621 atgttccgcc cggctggtga acatgcgata gggttcggtc ggcggcttag tcaccaggtc
     1681 atcgatcaac acgccgatgt acgcctggtc tcgccgcagg acaaaatccg ctaagccgca
     1741 agcggcgtaa cgcgccgcgt tgatgcccgc gatgatgcct tgcgccgccg cctcttcgta
     1801 accgctggtg ccgttgatct gccccgcgag gaacagtccg gcgatgcgct tggtttccaa
     1861 cgtcgaacga atctgtgtcg gccagaccat gtcgtattcg accgcgtagc cgtggcgaag
     1921 gatctccgct cgctccagcc ccggcaccag gcgaacgatc tgctcctgca catccgccgg
     1981 cagcgaggtg ctgatgccgt tgcagtaaat ctcgtcggtg tcgagtccct cgggctcgag
     2041 gaaaatctgg tgccgcgtct tgtcggcgaa gcgcacgacc ttgtcctcaa tgctcgggca
     2101 atagcgcggg ccggtcgatt gaatctgccc gctgtacatc ggcgcgcgat gcaggttggc
     2161 gcggatgatc tcgtgaatcc gctcgttcgt ccccgacaga tagcaaggca cctgcattaa
     2221 cggcggggtc cagccgcgaa agcccacgga tcgccatccg tgggcttcat cttcgtactc
     2281 attcaagtaa gaaaacggaa ccggcgcttc atcccccggt tgcagttcga atgctgcgaa
     2341 gtcgatcgac tgccgccgca accgcggcgg cgtgccggtc ttcaatcttc caagttccaa
     2401 tcccaacttt gccagacaac c
//
LOCUS       DATIMH010000768         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3648993, whole genome shotgun sequence.
ACCESSION   DATIMH010000768 DATIMH010000000
VERSION     DATIMH010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3648993"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VLI90_13440"
     CDS             <1..419
                     /locus_tag="VLI90_13440"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HSV15257.1"
                     /translation="FLNCKLTGDATPWDPATTNPASTVKPRVTPFADLGRPWRPYASV
                     TYINCEMGSHINPAGWNNWGKASNEATARYSEYHSTGPGAAAAKRVKWSKQLTDEQAK
                     SITVETVLSGNDQWNPIPIAQSLMQGSATRPNSAAQ"
     gene            523..1344
                     /locus_tag="VLI90_13445"
     CDS             523..1344
                     /locus_tag="VLI90_13445"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSV15258.1"
                     /translation="MSSASRAAELKTDVEYGRVGDTRLLLDASVPDGPGPFPAVIVLH
                     GGGWNSGDKQADAAPLLRALSASGSFTWFSINYRLAPANRWPACMDDLHTAIRWVKAH
                     AAEYKADPNRIALMGYSAGGQLVCLEAATAAEQNDADLAVQAVVGIAPPTDFEQDLPQ
                     RGGLSKSLQDLLNRPKEVTDESRQLIRSLSAINHIAPGAKLPPFLLVHGTVDKTVPFV
                     QSIAFQKRMRDAGATCDLVSVEGAPHRLSEWEKIDPTYKDKMIDWLKKTLAPAGK"
     gene            1460..>2136
                     /locus_tag="VLI90_13450"
     CDS             1460..>2136
                     /locus_tag="VLI90_13450"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HSV15259.1"
                     /translation="MVATDGSGDFKTIREAVAAAPDSSDERTVIHIKPGTYTGPLMVP
                     KAKAKISFEGDDAATTILSYDRNINEQPPDNWPVKYKGIGVVVLGDDFHASKVTFTNV
                     SGDHGQALALRLDGDRGVLENCRLIGWQDTLRLDDGRDYLKDCYIAGRVDFIYGSATA
                     VFDHCEIHSRNGGFVTAANTPQDKPFGFVFMNCKLTGDAIAWDPASTNPATTQKARVT
                     PMADLGRP"
BASE COUNT          425 a          652 c          720 g          339 t
ORIGIN      
        1 tgttcctgaa ctgcaagctg accggagacg cgacgccgtg ggacccggcg acgacgaacc
       61 ctgcgagcac ggtgaagccg cgcgtgacac ccttcgccga cctgggccgc ccgtggcggc
      121 cctacgccag cgtcacgtac atcaactgcg agatgggcag ccacatcaac ccagccggtt
      181 ggaacaactg ggggaaagcg agcaacgagg caacggcccg ctacagcgaa tatcacagca
      241 ccggccccgg cgccgcggcc gccaagcgcg tgaagtggtc gaagcagctg acggacgagc
      301 aggcgaagtc gatcacagtg gagacggtat tgagcgggaa cgatcagtgg aatccgatcc
      361 ccatagcaca gagcctgatg caggggtcag ccactcggcc aaactcagcg gcgcaatagc
      421 gatcgcggtt tcgtgagcgg tcgcatagaa gcagtgaccg aagaactttg tattgtgtac
      481 atgatgcgac atttccttgc ggcgagcctg gtcgttttcc tcttgagcag tgccagccgc
      541 gccgccgagt tgaaaaccga cgtcgagtac ggccgcgttg gcgacacgcg cttgctgctc
      601 gacgccagcg tgcccgatgg accgggccca ttcccggcgg tcatcgtgct gcacggcggc
      661 gggtggaaca gcggggacaa gcaggccgac gccgccccgc tgctgcgggc gctgagcgcc
      721 agcggcagct ttacgtggtt ctcaatcaac tatcgattgg ccccggcaaa ccgctggccg
      781 gcgtgcatgg acgatctgca cacggccatc cgctgggtga aggcgcacgc cgcggagtac
      841 aaggcggatc cgaatcgcat tgcgctgatg ggctactcgg ccggcgggca gctggtttgt
      901 ctggaggcgg caacggcggc ggagcagaac gacgcggacc tggcggttca agcggtggtg
      961 ggcatcgcgc cgccgacgga tttcgagcaa gacctgccgc aacgcggcgg gttgtccaag
     1021 tcgctgcagg acctgctgaa tcgcccgaag gaagtgacgg atgaatcgcg gcagttgatt
     1081 cgatcgttga gcgcaatcaa ccacatcgcg cccggcgcca agctgccgcc gttcctgctg
     1141 gtccacggca cggtggacaa gaccgtgccg ttcgtgcagt cgatcgcgtt tcaaaagcgg
     1201 atgcgtgacg cgggggcaac gtgcgatctg gtgagcgtcg aaggcgcgcc gcatcggctc
     1261 agcgaatggg agaagatcga cccgacgtac aaggacaaga tgatcgactg gttgaagaag
     1321 acgctcgcgc cggcggggaa ataactcggg agcttggacg actatgggtt cacaacagaa
     1381 gcgatttcgt tccgcccttg gcggccttgg tgtcttggcg gtcttggcgc tatctgcctc
     1441 cgcgcacgcc aagacgatca tggttgccac cgacggcagc ggcgacttca agaccattcg
     1501 cgaagccgtc gcggccgcgc cggattcgag cgatgagcgc acggtcattc acatcaagcc
     1561 cggcacttac accgggccgc tgatggtgcc gaaggcgaag gcgaagatct cgttcgaagg
     1621 tgacgacgcc gcgacgacga tcctcagcta cgaccgcaac atcaacgaac agccgccgga
     1681 caattggccg gtgaagtaca agggcatcgg cgtggtcgtg ctgggcgatg attttcacgc
     1741 gagcaaggtc acgttcacca atgtcagcgg cgatcatggt caagcgctgg cgcttcgcct
     1801 ggatggcgac cgcggcgtcc tcgagaactg ccggctgatc ggctggcaag acacgctgcg
     1861 gctggacgat gggcgcgatt acctgaagga ctgctacatc gccgggcgcg tggatttcat
     1921 ttatggctcg gccaccgcgg tcttcgatca ctgtgagatc cacagccgca acggcggctt
     1981 cgtgacggcg gcgaacacgc cgcaggataa gccgttcggc ttcgtcttca tgaattgcaa
     2041 gctgaccggc gacgcgattg cgtgggaccc ggccagcacg aacccggcga cgacgcagaa
     2101 ggcgcgcgtg accccgatgg cggacctggg ccgccc
//
LOCUS       DATIMH010000769         7945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5671499, whole genome shotgun sequence.
ACCESSION   DATIMH010000769 DATIMH010000000
VERSION     DATIMH010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5671499"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="VLI90_13455"
     CDS             <1..1406
                     /locus_tag="VLI90_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15260.1"
                     /translation="PSIGTGSFFSAALTVYGAKRIESAVGIELDAAFAKAARDLWAGA
                     GLNVVEGDFTRIVASGMRAPAPNLILANPPYVRHHHIDRHEKERLQRLAFELTGVEVN
                     GLAGLYVYFLLLATAWMEQDGYAAWLIPSEFMDVNYGASLKRFLTDQVTLIRAHRFDP
                     QDVQFGDAFVSSAVLVFKKATPPPGHEAEFTYGGTLNGPHASDRILLKPLRESRKWTA
                     YPTHAKNDRHTLSDCNSPTLADFFRVQRGIATGDNKFFILERDDATRRGLPARYLRPI
                     LPSPRNLKSTVIDADDEGYPLVDRQLCVIDCDLPEHVLQKRHPALWDYLKAGETSGVK
                     DGYLASKRRPWYRQEQREACPFLCTYMGRGTDEKRPFRFILNQSQAIGTNLYLMLYPR
                     GALLAMLKKHPHRAKEVHDLLCQITGHELRGEGRVYGGGLNKIEPSELGRVMAVSFVE
                     RWIELTRGAAATQLSLF"
     gene            complement(1438..2082)
                     /locus_tag="VLI90_13460"
     CDS             complement(1438..2082)
                     /locus_tag="VLI90_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15261.1"
                     /translation="MPATPVSPSKPRPASSTSEPASGVDGGASAPPTAPTDAAAEVAP
                     HVSAELVPEAVPEADLAQVTSAEAAELALTQPQRLAIVSLRNSPSRIKAAAAAQVTRQ
                     TLWRWMNQDPAFQAAYNAWQQDLIETAHGELLTGMRDAVQTLLRAARTDPKYAWRLLE
                     SQGVTQPPKPGSTDVQSVAEQQAMQKRVEVVKRKRMRVKLEMAEAMADLQMVNP"
     gene            2818..>7945
                     /locus_tag="VLI90_13465"
     CDS             2818..>7945
                     /locus_tag="VLI90_13465"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HSV15262.1"
                     /translation="MKQTKTTAKAASRFSLRADRVAPRRRAVVAAARRIVEHLETRTL
                     LSAATANTDTPLPEPYLPPQHDLLDLTGGYLSAPSSGAPLTIARNYLASHAASLGVSA
                     ADLGSSVVSDQYTDADTGTTHIYLTQTFDGLPVANANLVVNVTAHGQVLNVAGGFVNG
                     LAAAQGSGTQNGPVQLVTSASTPTISAVQALQDATADLGLSLSAQPTLVQSSSPVDMG
                     YGADETGTLTAPGVSSSDVPAGLQYIWTPDGVRLSWDLQIQTTDQAHWYDVSVGATAV
                     GSTGAGDLVFANDWVDHANYNVMALPNESANEGTRSVLTDPNDPTMSPYGWHDTNGVT
                     GPEYTITRGNNVFAYTDTANANVPGFSPDGGASLNFDYPWDTTQSPSTTSNQSAAVTN
                     LFYLNNTLHDIHARYGFTEAAGNFQVMNYSGQGVGNDPVLAEAQDGGGTNNANFATPV
                     DGSSPRMQMYLWTYSTPSRDGDFDNGIVVHEYGHGVSNRLTGGPANSNALSALQSGGM
                     GEGWSDWWALMLTQRPTDLQNSAYPMGTWALNQAATGAGIRRYPYSYNTSIDPETFGL
                     YGGANGTEVHNTGELWSTALWDMNWLLIQKYGFGTNVAGGYDPNTTGKNGGNNLALKL
                     VMDGLKLQPANPSFVDARNAIIAADQALNGGQDLFQIWSAFARRGLGYSAVDSSSSAT
                     TITTATDLPVGLLNPRVTAQSPTGAQTTPLSSIDFTFSKAMDPTSFSVAADVASFTGP
                     GAVDLKSAITGFSWINSNTTLHVTFSAQSTNGVYKMVIGPQILAGDNASPMDQDGDGT
                     AGEPTQDQYTATFGFESQFLQVASTTPANGSLVSLPFTTLDVKFNEPYDPATLGASDL
                     TLSQGTVTNAAVVDSTTARFTLSGITNEGTLTVAIAAGALSDTDGFGMLAYSGSFATD
                     IGTRAMPATTTLAPRGSMVYQSATSGIIGTTADTDSFTMSLDPGQTLSLVLHPTTAQL
                     RPSMQLYSPANVLLGTITAAAANQDAVIQSAPVAAAGTYTIVVASDGATSGSYALTAM
                     LNAAVEAEEHNGPSNDTIATAENMDSAFRVMAPGGPTIGSVVGNLATSATDFYAVSLT
                     AGQTVSFAVAMQSGTGTPALSLSNAAGTSLASGVSTITNVQSGIANYVVPTTAVYYLK
                     VTGSNVAYTLLATENAAFDFESNGTIATAQPVGGSRAVAGAVGSGIVGNVKVGLIQDQ
                     LPWGSSSDTTVATGLGYSVTVISSASLATANLSAYDMIVLSGQQTSATYNTVQTNLSR
                     IRSFVVAGGVYIANYAAYSTDNAYSYSVLPDAASGTFTYLGGSDINVLAPSSPLVNGP
                     GGTITDTTLDGGNYSFHGYTTVVPSGATTVLSGPSASQIVAFDYPDGAGHVIVDTIPI
                     EFYGGTGNFGIFHKNLFAYGGSFKPTNDDDYYAVTLAAGQTVQLGTSTPGDGAGEPGN
                     TLDPAIAIYDAGGNTLGADDNGGADGRNAHLSYTAATAGTYYVRVYPSPNSTTSGEYV
                     LNLFDKALTTTLPVSAQETAGAINGTLSVATAPTSDLVVTLTSSDSARVSVPSTLTIP
                     AGQTSVPLPITVLDNSLLDGLEQVTIAAVAGGYSSGNSVITIHDDETATLSVTLPSTA
                     REGDGVIVGTVTASAAPTRDIVVALSSDTLSKLSVPASVVIKAGQLSATFNATVIDNT
                     VIDGALNATVTAAVDNWTTGSSTIAVADNDAFITVTMPTSG"
BASE COUNT         1494 a         2570 c         2534 g         1347 t
ORIGIN      
        1 acccgtcgat tggcacgggc agcttctttt ccgcagcgct tacggtctac ggcgcgaagc
       61 gcatcgagag cgctgtgggc attgagctcg acgcggcatt cgcaaaagcc gcgcgcgacc
      121 tttgggcagg cgcgggtctc aacgttgtcg aaggtgattt cactcgcatc gttgccagcg
      181 gcatgcgcgc gcccgcgcca aatttgattc tcgccaatcc gccttatgtt cggcatcacc
      241 atatcgatcg ccacgagaag gagcgcctac aacgcttggc cttcgagctg accggcgttg
      301 aagtcaatgg gctggcgggc ctctatgttt acttcctatt gctcgccacc gcttggatgg
      361 aacaagacgg ctatgcggca tggctaattc cctcggagtt tatggacgtg aattacgggg
      421 cgtcgctcaa gcgattcctc acggatcagg tcacactcat tcgcgcgcac cgcttcgacc
      481 cgcaggatgt ccaatttggt gacgcgtttg tgtcgtcagc ggtcttagtc ttcaagaaag
      541 cgacgccgcc acccggacat gaggctgagt tcacatacgg cggtacgctc aatggtccgc
      601 acgccagcga tcgcattttg ctgaaaccgc tgcgtgagtc gcggaaatgg acggcctatc
      661 caacccatgc aaagaatgac cggcacacgc tcagcgattg caatagcccg acgctggccg
      721 acttcttccg cgttcagcgc ggcatcgcga cgggcgacaa caaatttttc atcttggaac
      781 gtgacgacgc gacgcgtcgg ggcctgcccg ccagatatct tcgcccgatc ctccccagtc
      841 ctagaaatct caagtccaca gttatcgacg cggacgacga gggctacccg ctggtcgatc
      901 gccaactttg cgtgatcgat tgcgatctgc ccgagcacgt cctgcaaaaa cgccatccgg
      961 ctttatggga ttatcttaag gccggcgaga cctccggtgt gaaagacggg tatctcgcca
     1021 gcaaacgccg cccttggtat cgtcaggagc agcgcgaggc gtgcccgttc ctatgcacgt
     1081 atatgggccg cggtacagat gaaaagcgac cattccggtt catcctgaat cagtcccagg
     1141 cgatcggcac gaacctctat ctaatgcttt atccgcgcgg cgccctgctt gccatgctca
     1201 agaagcatcc tcatcgcgcc aaagaggttc acgacctgct ctgccaaatc accggccacg
     1261 agctgcgcgg cgaggggcgc gtttatggcg gagggctgaa caagatcgag cccagcgagt
     1321 tgggccgcgt catggccgtc tcattcgtcg agcgctggat tgaactgact agaggagcag
     1381 cggccacgca gctctcgctg ttctagcaac gaaacgggcc gtgcctgccg ttcgccgtta
     1441 cgggttcacc atctgcaggt ccgccatcgc ttcggccatt tcgagtttga ctcgcatgcg
     1501 cttgcgcttg acgacttcga ctcgcttctg catcgcctgc tgctcggcga ccgattgcac
     1561 gtcggtggag ccgggcttgg gtggttgggt gacgccttgg ctttcgagca ggcgccaggc
     1621 gtatttgggg tcggtgcggg cggcgcgcag cagggtttgg acggcgtcgc gcatgccggt
     1681 gagcagttcg ccgtgggcgg tttcgatcag gtcctgctgc caggcgttgt aggcggcctg
     1741 gaaggcgggg tcctggttca tccatcgcca gagcgtctgg cgggtgactt gagcggcggc
     1801 ggcggccttg atccggctgg gactgtttcg cagcgagacg atcgccaggc gctgcggctg
     1861 ggtcagcgcc aactcggcgg cctcggcgct ggtgacctgg gcgaggtccg cttcgggaac
     1921 agcttcgggc acaagttcag cgctgacgtg cggggcaact tcagcggcgg catctgtcgg
     1981 cgcggtgggt ggcgcggatg cgccgccatc aacaccgctg gcgggttcgg aggtagacga
     2041 tgccggccgc ggcttcgaag gcgaaaccgg cgtggccggc aacggtggcg acgatgtctc
     2101 cgtgcgatgg tcccctgacg cgacgtgggg cttggatgta acatcgactt gggattggac
     2161 cggttgggta gaagggaagt gtttcatgac gaagatgcta acatcaacct aactactgtc
     2221 aagcagaaaa tatgaatcga cgtgaaataa tgggaatcaa tttggtccct aatttacagc
     2281 aaaattgctt aaaatgttac gttttcggtg cataacgtaa cattccgtaa catccgtaac
     2341 acggatgagg cgagttttga tggcggatca ctttcgccag ccaaacgctg acgaagacgc
     2401 cgccgcaggg ggtgaatgtg gtggcgaaaa tcgtgtcgga tcgagccggg ccagaggtga
     2461 cagacttgcg tcgctgaatg acgcgaagga catgggcgtg acggtggacg cccacccgcc
     2521 ggtgacgcgg gagtggcggg gttatccact tgagacttga cggccaacgc ggtagcgttt
     2581 gtgcgtccgt tcgtttcgcc aaagaggggt ggcgcgcggg gtgcaagtca tcggggctgc
     2641 cccctacgta attaccagca gtaaatagca agacggcgta tcgacgagcc tgtataactg
     2701 ctccgcgttt caagacgcgt cggggtcgat caagaaactc agttgggcat gcggggatgc
     2761 gcgcgggtac gcggggacgg acgggtaggg catttttggg gcggggaaac acacatcatg
     2821 aaacaaacga agaccacggc caaggcagct tcccggttct cactgcgcgc agatcgagtg
     2881 gcgccgcggc gccgcgcggt cgtggcggcg gcgcggcgga tcgtcgaaca cctcgagacc
     2941 cgcacgctgc tgagcgctgc caccgccaac accgacacac cgctgcccga accgtatctg
     3001 ccgccgcagc atgacctgtt ggacctgacc ggtggatatc tgtcggcgcc gtcgagcggt
     3061 gcgccgctga cgatcgcgcg caattacctc gcatcgcacg cggcatcgct gggcgtgagc
     3121 gcggcggatc tgggcagttc cgtcgtcagc gaccagtaca ccgacgccga caccggcacc
     3181 acgcacatct acctgacgca aacgttcgat ggcctgccgg tggccaacgc caacctggtg
     3241 gtgaacgtga ccgcccacgg gcaggtgttg aacgtggcgg gtggttttgt gaacgggctg
     3301 gccgcggcgc aaggatcggg gacgcagaat ggaccggtgc agctggtgac cagcgcgtcc
     3361 acgccgacga tcagcgcggt gcaggcgctg caggatgcga cggccgattt gggattgtcg
     3421 ctgagcgctc agcccacgct ggtgcaatca tccagtccgg tggacatggg atacggcgcg
     3481 gacgagacgg gcacgctgac ggcgccgggt gtttcctcaa gcgacgtgcc ggcggggttg
     3541 caatatatat ggacaccgga cggcgtccgg ctgtcgtggg atctgcagat ccaaaccacc
     3601 gatcaagcgc attggtatga cgtcagcgtc ggggcaacgg cggtgggcag caccggcgcg
     3661 ggcgatcttg tctttgccaa cgattgggtt gatcacgcga actacaacgt gatggcgctg
     3721 cccaacgaaa gcgcgaacga aggcacgcgc tcggtcctga ccgaccccaa cgacccgacg
     3781 atgtcgccct acggttggca cgacaccaac ggtgtgaccg ggcccgaata cacgatcacg
     3841 cgcgggaaca acgtcttcgc ctacaccgac acggctaacg cgaacgtccc gggcttcagc
     3901 cccgacggtg gcgcttcgtt gaacttcgat tacccgtggg acacgaccca gtcgccgagc
     3961 accacctcga atcaatcggc ggcggtgacg aacctgttct atctgaacaa cacgctgcac
     4021 gacatccacg cgcgctacgg gttcaccgaa gccgccggca atttccaggt gatgaattat
     4081 tcggggcagg gggtcggcaa tgatccggtg ctggccgaag cgcaggacgg cggcgggacg
     4141 aacaacgcca acttcgccac gccggtggat ggttcgtcgc cgcgcatgca aatgtatttg
     4201 tggacgtatt ccaccccgtc gcgcgacggt gatttcgaca acggcatcgt cgtccacgaa
     4261 tatgggcatg gcgtttccaa tcgattgacc ggtggcccgg ccaactccaa cgcgctcagc
     4321 gccctgcaaa gcggcgggat gggtgaagga tggagcgact ggtgggcgct gatgctgacg
     4381 cagcggccaa cggacctgca gaattcggcg tatccgatgg gcacgtgggc gttgaaccag
     4441 gccgccaccg gcgccggcat ccggcgctat ccgtactcgt acaacaccag catcgacccc
     4501 gaaacgttcg ggctatacgg cggggccaac ggcacggaag tgcacaacac cggcgagctg
     4561 tggtccaccg cgctgtggga catgaactgg ttgctgatcc agaagtacgg cttcggcacg
     4621 aacgtcgccg gtgggtacga ccccaacacc accgggaaga acggcggcaa caacctggcg
     4681 ctgaaactgg tgatggacgg cctgaaactg cagccggcca atccgtcgtt cgtcgatgcg
     4741 cgcaacgcga tcatcgcggc cgaccaggcg ctcaacggcg ggcaggattt gttccagatc
     4801 tggagcgcct tcgcccgccg cgggctgggg tacagcgcgg tggacagcag ctcgagcgcg
     4861 accaccatta caacggcgac cgatctgccg gtggggttgc tcaatccacg cgtgactgcg
     4921 cagagcccga ccggcgcgca gaccacgccg ctcagcagca tcgacttcac gttcagcaag
     4981 gcgatggacc cgacgagctt cagcgtcgcc gccgacgtcg ccagcttcac cggccccggc
     5041 gcggtcgatc tgaaatccgc catcaccggc ttctcgtgga tcaacagcaa caccaccctg
     5101 cacgtgacgt tcagcgcgca atcaaccaac ggcgtctaca agatggtgat cggcccgcag
     5161 atcctcgccg gtgacaacgc ctcgccgatg gaccaggacg gtgacggcac ggccggcgaa
     5221 ccgacgcagg atcagtacac ggcgacgttc ggtttcgagt cgcagttcct gcaggtggca
     5281 tcgaccacgc cggcgaacgg ttcgctggtc agcctgccgt tcacgacgct ggatgtgaag
     5341 ttcaacgagc cgtacgaccc cgctacgctc ggcgccagcg acctgacgct gagccagggc
     5401 actgtcacca acgccgccgt cgtcgattcg acgaccgcgc ggttcacgct cagcggcatc
     5461 accaacgaag gcacgctgac cgtcgcgatc gcggcgggcg cgctctccga caccgacggt
     5521 ttcggcatgc tggcgtacag cggatcgttc gcgaccgaca tcggcacgcg cgcgatgccc
     5581 gccaccacca ccctcgcgcc gcgcgggtcg atggtgtacc agtcggcgac gagcggcatc
     5641 atcggcacga ccgccgacac cgacagcttc acgatgtcgc tggacccggg gcagacgctg
     5701 agcctcgtgc tgcacccgac gaccgcgcag ctgcgcccgt ccatgcagct gtacagtcca
     5761 gccaacgtcc tgctcggcac catcaccgcc gccgcggcga accaggacgc ggtcatccaa
     5821 agcgcgccgg tggcggccgc ggggacgtac acgatcgtcg ttgccagcga cggcgccacc
     5881 agcggttcat acgcgctgac ggcgatgttg aacgctgcgg tggaagccga agagcacaac
     5941 ggcccgagca acgacacgat cgccaccgcg gagaacatgg actcggcctt ccgcgtgatg
     6001 gcgccgggcg gtccgacgat cggatcggtc gtcggcaacc tcgccacctc cgcgacggac
     6061 ttctacgccg tctcgctgac ggcggggcag acggtctcgt tcgccgtcgc catgcaatcg
     6121 ggcacgggca cgcccgcgct gtcgctgtcc aacgcggcgg ggacctcgct ggcgagcggt
     6181 gtgagcacga tcaccaacgt gcagtcgggc atcgccaact acgtcgtgcc caccacggcg
     6241 gtgtattact tgaaggtcac cggcagcaat gtggcgtata cgttgctcgc cacggagaac
     6301 gcggcgttcg acttcgaaag caacggcacg atcgccaccg cgcaaccggt cggcggcagc
     6361 cgcgcggttg ccggggccgt cggcagcggc atcgttggca acgtgaaggt cgggctgatc
     6421 caggaccagc tgccgtgggg cagctcttcc gataccaccg tcgccaccgg cctcggttac
     6481 agcgtgacgg tcatcagctc ggcctcgctg gcgacggcga acctcagcgc gtacgacatg
     6541 atcgtcctct cgggccagca gaccagcgcg acctacaaca cggtgcagac gaacctgtcg
     6601 cgcatccgtt cgttcgtggt cgccggcggt gtgtacatcg ccaactacgc cgcgtattcc
     6661 accgacaacg cctacagcta tagcgtgctc ccggacgcgg cttcaggcac cttcacgtac
     6721 ctcggcgggt ccgacatcaa cgtgctcgcg ccctccagcc cgctggtcaa cggtcccggt
     6781 ggcacgatca ccgatacgac gctcgatggc ggcaattatt cgttccacgg atacaccacc
     6841 gtcgtgccca gcggcgcgac caccgtcctt tccggtccca gcgcgtcgca aatcgtcgcc
     6901 ttcgattacc ccgacggcgc cgggcacgtc atcgtcgata cgatcccgat cgagttctat
     6961 ggcgggacgg gcaacttcgg catcttccac aaaaacctgt tcgcctacgg cggcagcttt
     7021 aagccgacca acgatgacga ttactacgcc gtgacgctgg ccgcggggca gacggtgcaa
     7081 ctgggcacct ccacgccggg cgacggcgcg ggcgagccgg gcaacacgct cgatccggcc
     7141 atcgcgatct acgacgccgg cggcaacacg ctgggcgccg acgacaacgg cggcgccgac
     7201 ggccgcaacg cccacctttc ttacaccgcc gccaccgccg gcacgtatta cgtgcgcgtt
     7261 tatcccagcc ccaacagcac gacctccggc gaatacgtcc tgaacctgtt cgacaaggcg
     7321 ttgaccacca cgctgccggt cagcgcgcag gaaaccgccg gcgcgatcaa cggcacgctg
     7381 agcgtcgcca ccgcgcccac cagcgatctg gttgtgacgc tgacctccag cgacagcgcg
     7441 cgcgtctcgg ttccgtccac gctgacgatt cccgcggggc aaacttcggt cccgctgccg
     7501 atcacggtcc tggacaactc gctgctggac ggcctggagc aggtgacgat cgccgcggtg
     7561 gccggtggct attcatccgg caattccgtc atcacgatcc acgacgacga aaccgccacg
     7621 ctcagcgtga ccctcccgtc aacggcgcgc gaaggggacg gcgtgatcgt cggtacggtc
     7681 acggcgtcgg cggcgccgac gcgcgacatc gtggtcgcgc tttcgtccga cacgttgtcg
     7741 aagctctcgg tgccggccag cgtcgtgatc aaggccgggc agctcagcgc gacgttcaac
     7801 gcgacggtga tcgacaacac ggtgatcgac ggcgcgctga acgcgaccgt caccgcggcc
     7861 gtcgataact ggaccaccgg ctcctcgacc atcgcggtcg ccgacaacga cgcgttcatc
     7921 accgtgacca tgcccaccag cgggt
//
LOCUS       DATIMH010000770        10432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2782460, whole genome shotgun sequence.
ACCESSION   DATIMH010000770 DATIMH010000000
VERSION     DATIMH010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2782460"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1034)
                     /locus_tag="VLI90_13470"
     CDS             complement(48..1034)
                     /locus_tag="VLI90_13470"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HSV15263.1"
                     /translation="MTEIAEQSTAVGFAANGSFSMPLKFPSPVTSPITDAARPLALRT
                     GYDMHFEVLGTQPTAMLLMLFVQPEVAAHLDRPERLIVEPAGVEVQSFFDAFGNRCGR
                     IVAPPGPLRLTYDAVYTGTYAPEPQPSVDARQYPAGDLPHEVLPFLLGSRYCEVDRLS
                     AIAWDLFGKISHGGERVLAINQWVFKNIAFGYKLTRPNKTALDTYVERTGVCRDMMHL
                     AITFCRALNIPGRYASGYLGDIAADPDPTPMDFSAFYEVYLDGRWWPMDARHGKARVG
                     RVPMARGRDATDVALITSFGRHRLTKFTVATDEIISPPSKLTPAPLPFRLSA"
     gene            1222..1839
                     /locus_tag="VLI90_13475"
     CDS             1222..1839
                     /locus_tag="VLI90_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSV15264.1"
                     /translation="MKASAAKTRPRRREYTQTARAEAAQETGRRIVDAFVDRLVSGWF
                     DEITLDRIADVAGVTVQTIIRRFGGKEGLLGDAVTVLKERITAQRGAPAGDVDTMVRN
                     LYADYERTGDAVMRVLALELRHPVVTEITNAGRREHRLWVADAMAPALNKVPSGSQRH
                     RFLDELVIVTDVYTWKLLRRDMRHSLLDSTAIATSMVHAVVASHQ"
     gene            2008..3297
                     /locus_tag="VLI90_13480"
     CDS             2008..3297
                     /locus_tag="VLI90_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV15265.1"
                     /translation="MPQKLNYLFTSWEGGGNVTPLLVVAGKLTSRGHRVRVQGDVCNR
                     PEAEAAGAAFVPWNRAPNRPDRTPATQPYNDWDVPSPQEGIMHVVRDVWCGPALRYAQ
                     DVIDELRREPADLVVTSEMLYGVMAGCESIGQPFVTFAPNIRLAPLPGVPPMGPGLPP
                     ARNEQERDMHRQIEAGSQGLFDAGLPALNAARVELGLAPLEHLLDQLEVAEAELLGTS
                     RAFDFPAASLPPKVRYVGPQIGDPNWAKPWQSPWPASDTRPLVSVSFSTTFQGHTGVL
                     QKVIDALATLPARVLVTLGGSVDAADLRRAANTLIVDSAPHSQVMREASLVVTHGGHG
                     TVMRALMSRVPMLVIPHGRDQNDNAIRVTERGAGLSLQANASVDVIRDACAKILADPS
                     FKAAATRLGDLVAAEAERSRVVEELESIAARATLSAV"
     gene            complement(3403..8982)
                     /locus_tag="VLI90_13485"
     CDS             complement(3403..8982)
                     /locus_tag="VLI90_13485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV15266.1"
                     /translation="MSLDLHARHERLNTVGCNGRSSRRRLASAKTALAIATGNAAILL
                     GAQGALAANQSWVGTTDANWTTVTNWSGGAVPGINPTGNSTNTTDVATFNAALSVVGG
                     TTIGDINNPILIDGTASSSGSRMIGRLTFDTANAGAYYIGAASGSPNLQLGNNLGINM
                     TATVVNPEVVQAPISLHLPSSTNGVGSITNDSTTSSATLTIASITNSLNSTRGTAWTL
                     GGANTGNNVVHSISISNTGGVSSSVTKTGAGRWILDGANTIPGGVTVSQGTLAVTNAG
                     ALGSANANVGSVNGGILEIDGVALSNTSVTLTSGGTLLANGAAESAKLVPVAAAATNV
                     TLSTTNAGDVFTIGNGTNSITGGTAGTSVLHTSGPGTIVLSNAGTYAGSWSIDAGTVN
                     LGSATALGPAATATVAFGAGSTGKLQLNSNIVTLAQLNTNATPGSAIVENGGGSAAVL
                     TVNNTAANTFAGVLGDGTGGGSLGLTKTGAGNFTLTGANTYSGNTTIGGGTLLANNGA
                     GSATGSSNVAVNSGGTLGGTGLISGAVTLASGGHLSPGSGGVGTLSVGSLTLNGGSQL
                     DYDITNTSTLDKTLVTTNGGLTINGGQLNINGGVAAFTTNGVYNLIGFSGAIGGSGAG
                     SLTVNPNNESLATNTYTIGSAGGFVTLAVASSGGSIIYWNADADGNWSTGPWTGSTPN
                     SPGAFASFGGGGVPITANRTISVDGSNTVGTLAFNNPSFSYTLAAGSGANITLDNSAA
                     GAFVTDSAGNHTIQSPLTLTSNGATFTVSNPGDTLTVSGAIGGSGDVLTKAGTGTLVL
                     SGTNAYSGGTNLNAGTIVINSATSLGDPAGNASFGGGTLKLAADVVTARNYVVTGSAD
                     AIIDTNGHTLTQGGTVSAGSGATGGLTKNGAGTLLLQGTNSYVGNTTINAGTVSVASN
                     ANLGDPATGAAINLGGGTLATTATLALDNGAGANARAINTGAVGGTIDVADQTTLTVA
                     GAITGAGVVTKTDTGTLVVSNTANTAPFIVSAGTLTAAGTTANTNGGFGSGAITLQGG
                     ATLSALMPTGNTLGFGNALTVPAGQTGTIDMTNRFSMSGAVSGAGTLNLVASSTISRD
                     DFSNNWAGFIGNLNISGSGNVRFVMNGGGFNAAGLASTTLDLEGTAFTSPVTNSTGNT
                     VTIGALSGISTTAGLGGGSAGAPNYTVGGLNTSTTFAGLITGNSILTKVGTGTLTLTN
                     ANSYSGLTNINGGTLNINGQFALGGAVYAGTAFNGGTLQYAASLPGTNGTGDITQDSN
                     AAAKPVTLGANGGTVDTNGNDITYANPVGAGAGAFTKAGAGTLTLAAATSYTGNTNIT
                     GGTLALSGSGAITNGTINLNGGSFDVTGVSASPYVIGAAQTLKGQGLVNGNVTVNGTV
                     SIGQTINTAGKVAKTLTIGSGSTLTGSLLEDLTAVNASDKLVFGDGTTSTANLGGVLT
                     LTNPNAVSFAAGQNYDLLDFGSETGTFSTVTLPTLSGALSWDTSHLYSTGVVSIITSG
                     PANLTWNNSGGTGNGTLWDTAGQQNWNNGSAPAQFNAADNVTFNDTNNNHYAVTLNST
                     VTPGSVTVNNSAGDYTISGTGAIAGSGGLTKTGTSALTISTVNTYTGNTTVNGGTLTL
                     DAGGAIASSTITVTAPGVANINGAVPSTVTVNANGTTNFGGNSTNAPVTRQIGTLNIG
                     AATASVAHSTFPGSPAILIPSAVNFNDPAAVLDLTDNELLAPGDASVAEGLIVAGKLV
                     SSEADSTHALGYITAGGNMEVRYTLKGDANMDGTVNVGDLGALATSYNITGGMSWANG
                     DFNHDDTVNVADLGALATNYNISMATGASAGGGAASSIASPMAVASGAAAVPEPASAT
                     LLALGAAATLIRRRRRA"
     gene            complement(9374..10174)
                     /locus_tag="VLI90_13490"
     CDS             complement(9374..10174)
                     /locus_tag="VLI90_13490"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSV15267.1"
                     /translation="MICSAFVASSACAQTSQPAGNFKPLFNGKDLTGFYTYLKGDGKN
                     NDPQKVFQVDRDGVVHIYRDSTDGAAMPFGYFATEEEFSDYHLRFQYKWGTKRFGGRS
                     KSRRDAGCLYHVTGPDGWLNGTWPFSIECQVMEQDTGDIFAVGTSVTTMIDPAKAKTP
                     TFMEATAGGIEYTSPQKMPVNSRVIRDPMAEVDGWNTVEIIVRGDSAVHIVNGRVNNR
                     ILHATVPNLDKPGEWVPLRKGRILFQAEGAEVCYRNIEMHPVVTDEVR"
BASE COUNT         1717 a         3225 c         3524 g         1966 t
ORIGIN      
        1 cggctaatct attccgggct gacaacgagc gaatgggcgc gtcgctctca ggcgcttagc
       61 ctgaacggca gcggcgccgg ggtgagcttt gatggcggcg aaatgatctc gtcggtcgct
      121 accgtgaact tcgtcagtcg atgccgcccg aagctggtga tcagcgccac gtcagtcgcg
      181 tcgcggccgc gggccatcgg cacgcggccg accctggcct tgccatgccg ggcatccatc
      241 ggccaccagc ggccgtcgag atacacctca tagaacgcgc tgaaatccat cggcgttgga
      301 tccggatcgg cggcgatgtc gcccagataa ccgctcgcgt atcgcccggg aatattgagc
      361 gcccggcaga aggtgatcgc caggtgcatc atgtcccggc agacgccggt gcgctcgacg
      421 taggtatcca gcgccgtctt gttcggccgg gtgagcttgt aaccgaacgc gatgtttttg
      481 aagacccatt gattgatcgc cagcacgcgc tcgccaccgt gggagatctt gccgaacagg
      541 tcccacgcga tggccgacag gcgatcgact tcgcaatatc ggctgccgag caggaacggc
      601 agcacctcgt gcggcagatc gcccgcgggg tattggcgcg catcgaccga aggttgaggt
      661 tcgggggcgt aggtgccggt gtagaccgcg tcgtacgtca accggagcgg gcccggtggc
      721 gcgacgatgc ggccgcagcg gttgccgaag gcatcgaaga aactctgcac ctccacgccc
      781 gccggttcca cgataaggcg ctcgggacga tccaaatgcg ccgctacttc cggctgcacg
      841 aagagcatca gcagcatcgc cgtcggctgc gtgcccagga cttcgaaatg catgtcgtag
      901 ccggtgcgca gcgccagcgg ccgagccgcg tccgttattg gactcgtcac aggcgacggg
      961 aacttcagcg gcatcgagaa tgaaccgttg gcagcgaagc ctacggcggt ggattgctcg
     1021 gcgatctcgg tcacgttgtc taactcctct ccatcgtgcg cacacaagtt cgcgtcccgt
     1081 gctagcccat aagatcggcc aaaagcaaat tccatttcgc ggaattacgc acgtccgata
     1141 acacgcgacc ggagaacgtg agaaagagcc attggaaaaa atctccttga ctcgatggta
     1201 ttcgatgtta gtataactaa catgaaagcg agcgccgcca agaccagacc ccggcgacgt
     1261 gaatacacgc agacggcgcg ggcggaagcg gcgcaggaga ccggtcggcg catcgtcgat
     1321 gcgttcgtcg atcgcctggt gtccggttgg ttcgacgaga tcacgctcga tcgcatcgcg
     1381 gacgtcgcgg gcgtgacggt gcagacgatc atccgccggt ttggcgggaa agaggggctg
     1441 cttggcgatg cggtgacggt cctaaaggaa cggatcaccg cccaacgcgg cgccccggcc
     1501 ggtgatgtcg atacgatggt gcgcaatctc tacgctgact acgagcgcac cggcgatgca
     1561 gtcatgcgcg tgctggccct ggaattgcgg catccggtgg tcaccgaaat aaccaacgcc
     1621 ggccggcgcg agcaccggct ctgggtggcc gacgcaatgg cgccggcgct gaacaaggtg
     1681 ccatcagggt cccagcgcca tcgcttcctg gacgagctgg tcatcgtcac tgatgtctac
     1741 acctggaagc tgcttcgccg cgatatgcgg cacagcctcc tggattcgac cgcgatagcg
     1801 acatcaatgg tccacgcagt ggttgcaagt catcaatagg agttcaagtc tcgaacaccg
     1861 ttttagcggc ggcccgaagg gccgagcgtt ctgcgcgcag actgtgtgtg ttcgacaatc
     1921 gcgactggta atttcgaacg cgttcggtaa ctgacatctc tctagcaaag aaattctgca
     1981 acggatcatt tcacaaggag accgcccatg ccgcagaaac tgaattacct cttcaccagt
     2041 tgggaaggcg gcggaaatgt cacgccgctg ctcgtcgtcg ctggcaagct cacctcccgc
     2101 ggccaccgcg ttcgcgtcca aggcgacgtc tgcaatcgcc ccgaggccga ggcggcgggg
     2161 gccgccttcg tcccctggaa tcgcgcgccc aatcgtccgg atcgcacgcc ggcgacgcag
     2221 ccttacaacg actgggacgt gccgtcgccg caggaaggaa ttatgcacgt cgtgcgcgac
     2281 gtgtggtgcg gaccggcgct gcggtatgcg caagacgtga tcgacgagct gcgccgggaa
     2341 ccggcggacc tggtggtgac atcggagatg ctgtacggcg tgatggccgg ttgcgaaagc
     2401 atcgggcaac cgttcgtcac gttcgcgccg aacatccgct tggcgccgtt gccgggcgtg
     2461 ccaccgatgg gaccgggact gccgccggcg cgcaacgagc aggagcgcga catgcatcgg
     2521 cagatcgagg cggggtcaca gggactgttc gacgcgggat tgccggcgct gaacgccgcc
     2581 cgcgtcgagt tgggcctggc gccgctggag catctgctcg atcaactgga agtggccgag
     2641 gccgagttgc tggggacgag ccgcgcgttc gacttccccg cggccagctt gccgccgaag
     2701 gtgcggtacg ttggcccgca gattggcgat ccgaattggg ccaagccgtg gcagtcgccg
     2761 tggccggcgt cggacacgcg gccgctggtg agcgtgagct tctcgacgac gttccaggga
     2821 cacaccggtg tgttgcagaa ggtgatcgat gcgctggcaa cgctgccggc gcgcgtcctg
     2881 gtgacgcttg gcggatcggt ggacgccgcc gacctgcggc gggcggcgaa cacgctgatc
     2941 gttgacagcg caccgcactc gcaggtcatg cgcgaggcgt cgctggtcgt cacgcacggc
     3001 ggacacggga cggtcatgcg cgcgctcatg agccgggtgc cgatgctggt catcccgcac
     3061 ggccgcgacc agaacgacaa cgcgatccgc gtgaccgagc gcggcgcagg tctgtcgctg
     3121 caggcgaacg ccagcgtgga tgtgatccgc gatgcgtgcg cgaaaatcct ggccgacccg
     3181 tcgttcaagg cggcggccac gcgcttgggg gatctcgtcg ccgctgaagc ggagcggtcg
     3241 agggtcgttg aagagcttga gagcatcgct gcccgcgcaa cgctctccgc cgtatgagga
     3301 tgacagagca cgcggggagc cgctgtcact gaaaaagagt gcggcggccc gctggtcaga
     3361 gacgggccgc cgcgcggagg gatcgagaaa acgttcagtt actcatgcgc gccgccggcg
     3421 ccggatgagc gttgcggcgg cgccaagcgc gagcaatgtt gccgacgccg gttccggcac
     3481 ggcggccgcg ccgctggcga ctgccattgg cgatgcgatg gaggacgccg caccaccgcc
     3541 cgccgacgcg ccggtggcca tcgagatgtt gtagttggtg gccagcgcgc ccaggtccgc
     3601 gacgttgacg gtgtcgtcgt gattgaagtc accgttcgcc cagctcatgc cgccggtgat
     3661 gttgtagctg gtcgccagcg cgcccagatc accgacgttc accgtgccgt ccatgttcgc
     3721 gtcgcccttg agcgtgtagc gcacttccat gtttccgccg gccgtgatgt agcccaaggc
     3781 gtgcgtgctg tcggcttcgg aactgaccaa cttgccggcc acgatcaagc cttcagcgac
     3841 gctcgcatca ccgggggcga gcagttcgtt gtcggtcaga tccagcacgg cggccggatc
     3901 gttgaagttg accgccgacg gaatgaggat cgccggcgaa ccggggaacg ttgagtgcgc
     3961 caccgacgcc gtggcggcgc cgatgttcaa cgtgccgatt tgccgggtga caggagcatt
     4021 ggtgctgttg ccaccgaagt tcgtcgtgcc gttggcgttc acggtgacgg tgctcggcac
     4081 cgcgccattg atgttggcca cgcccggcgc ggtgacggtg atggtgctgc tggcgatcgc
     4141 gccgcccgcg tccagcgtca gggtaccgcc gttgacggtg gtgttaccgg tgtaggtgtt
     4201 gacggtggag atcgtcaacg cgctggtgcc ggtcttggtt aaaccaccgc tgccggcgat
     4261 cgcgccggtc ccgctgatgg tgtagtcacc ggcggagttg ttgacggtga ctgatccggg
     4321 ggtcacggtg ctgttgagtg tcaccgcgta gtgattgttg ttggtgtcgt taaaggtcac
     4381 gttgtcggcg gcgttgaact gcgcgggagc cgatccgttg ttccagttct gctgcccggc
     4441 ggtgtcccac aacgtgccgt tgccggtgcc gccgctgttg ttccaggtca ggttcgccgg
     4501 accgctggtg atgatggaaa ccacaccagt gctgtacaag tggctggtgt cccagctgag
     4561 cgcgccgctc aacgtgggga gggtgacggt gctgaacgtg ccggtttcgc tgccgaagtc
     4621 gagcaggtca taattttgcc cggccgcgaa gctgacagcg ttggggttgg tgagggtgag
     4681 cacgccgccc aggttggcgg tgctggtcgt cccgtcgccg aacaccagct tgtccgatgc
     4741 attcaccgcc gtgaggtctt ccagcaggct gccagtcaac gtcgagccag agccgatcgt
     4801 cagcgtcttg gccaccttgc cggcggtgtt gatcgtctgg ccgatcgaca cggtgccgtt
     4861 cacggtgaca tttccgttga cgagcccctg gcccttgagc gtttgcgccg cgccgatgac
     4921 gtatggcgag gcggacacgc cggtcacatc gaacgaaccg ccgttcaggt tgatcgtgcc
     4981 attggtgatc gcgcccgagc cgctgagcgc gagcgtgccg ccggtgatgt tcgtgttgcc
     5041 ggtataggac gtggccgcgg cgagcgtgag ggtgcccgcg cccgctttgg tgaacgcccc
     5101 tgcgccggcg ccgacggggt tggcgtacgt gatgtcgttg ccgttggtgt cgaccgtgcc
     5161 accgttggcg ccgagcgtca cgggcttggc agcagcgttg ctgtcctgcg tgatgtctcc
     5221 ggtgccgttg gttcccggca gggatgcggc gtactgcagc gtaccaccgt tgaacgccgt
     5281 tccggcgtag accgctccgc ccagggcgaa ctgcccgttg atgttgagcg tgccaccgtt
     5341 gatgttggtc aacccgctat aggagttggc gttggtcagc gtgagggtgc ctgtgccgac
     5401 cttggtcagg atggaattgc cggtgataag accagcgaac gtggtgctgg tgttgagccc
     5461 accgacggtg tagttgggcg cgcccgcgct acctccaccc aagccggccg tcgttgagat
     5521 gcccgagagc gcgccgatgg tcaccgtatt gcccgtgctg ttggtcaccg gcgaggtaaa
     5581 cgccgtgcct tccagatcca gcgtggtaga ggcgagcccc gcagcgttga aaccaccgcc
     5641 gttcatgacg aagcgaacat tgcctgaacc gctgatgttg aggtttccaa tgaagccagc
     5701 ccagttattg ctgaagtcat ctcgggagat cgtggacgag gccaccaggt tgagcgtgcc
     5761 ggcgccgctg acggcgcccg acatgctgaa acgattggtc atgtcaatcg tgccggtttg
     5821 acccgccggc acagtcaacg cattcccgaa gcccagcgta ttgccggtcg gcatcaacgc
     5881 actcagcgta gcgccccctt gcagggtgat cgcgccacta ccgaatccgc cattggtatt
     5941 ggcggtcgtg ccagcggcgg tgagcgtgcc ggccgacacg atgaacggcg cggtgttggc
     6001 ggtgttggag accactagcg tgccggtgtc ggtcttggtc accacgccag cgccggtgat
     6061 cgcgccggcg acggtgagcg tcgtctggtc ggccacgtca atcgtgccgc cgacggcgcc
     6121 ggtgttgatc gcccgtgcgt tggcgccggc gccgttgtcc agtgcgagcg tcgcggtggt
     6181 ggccagcgtg ccgccgccaa ggttgatcgc cgcgccggtc gccgggtcac cgaggttggc
     6241 gttgctggca acgctgacgg tgcccgcatt gatcgtcgtg ttaccgacgt agctgttggt
     6301 gccttgcagc agcagcgtgc ccgcgccgtt cttggtcagg ccgccggtcg cgccgctgcc
     6361 ggctgacacg gtgccgccct gagtgagcgt gtgcccgttg gtgtcgatga tcgcatcggc
     6421 gctgccggtg acaacgtaat tgcgagcggt caccacgtcc gccgccagct tcagggtacc
     6481 gccgccgaag ctcgcattgc ccgcggggtc gccgaggctc gtggcactgt tgatgacaat
     6541 cgtgccggcg ttgaggttcg tcccgccgga gtaggcgttg gtcccgctca gcaccagcgt
     6601 gccggtgccg gccttggtga ggacgtcgcc gctgccgccg atcgcgcccg aaaccgtgag
     6661 cgtgtcaccg ggattgctga cggtgaacgt cgcgccgttg ctggtcagcg tgagcgggct
     6721 ctgaatcgta tggttgcccg ccgagtcggt gacgaacgcg ccggccgccg aattgtccag
     6781 cgtgatgttc gcgccgctcc cagccgccag cgtgtaggag aagctggggt tgttgaacgc
     6841 cagcgtgccg acggtgttgg acccatcgac gctgatcgtg cggttggcgg tgatcggaac
     6901 gcccccgccg ccgaatgacg cgaacgcgcc cggtgagttg ggggtgctgc cggtccaggg
     6961 accggtgctc cagtttccgt cggcgtcggc gttccagtag atgatcgagc caccgctgct
     7021 ggcgacggcc agcgtcacga aaccaccggc cgagccgatc gtgtacgtgt tggtcgcgag
     7081 gctttcgttg ttggggttaa cagtcagcga accggcgccc gaccctccaa tcgcgccgct
     7141 gaagccgatc aggttgtaca cgccattggt ggtgaacgcc gccacaccgc cgttgatgtt
     7201 gagctgcccg ccgttgatcg tcagaccacc gttggtggtg accagcgtct tgtccagcgt
     7261 gctggtgttg gtgatgtcgt agtcgagttg tgagccgccg ttgagcgtga gcgacccgac
     7321 gctgagcgtg ccgacgccgc ccgaaccggg cgacaaatga ccaccgctgg cgagggtgac
     7381 ggcgcccgag atcaagccag tgccaccgag cgtgccaccg ctgttgacag ccacgttgga
     7441 cgagccggtc gccgagccgg ccccgttgtt ggccagcagc gtcccgccgc cgatggtcgt
     7501 gttgccgcta taggtgttgg ccccggtgag cgtgaagttg cccgcgccgg ttttggtcag
     7561 gccgagcgaa ccaccgccgg tgccgtcgcc gaggacgccg gcaaaggtgt tggcggccgt
     7621 gttgttgacg gtcagcaccg cggctgatcc gccaccgttc tcgacgatgg cgctgccggg
     7681 cgtggcgttg gtgttgagct gcgccagcgt gacgatgttc gagttcagtt gaagcttgcc
     7741 ggtgctgccg gcgccaaagg caaccgtcgc ggtcgcggcc gggcccagcg ccgtggcgct
     7801 gccgagattc accgtgccgg cgtcgatcga ccaggaaccg gcgtacgtgc cggcattgga
     7861 cagcacaatg gtgccgggac ccgaggtgtg caacacgctc gtccccgcgg tgccgccggt
     7921 aatcgaattg gtcccattgc cgatggtgaa cacgtcaccg gcgttggtcg tgctcaaggt
     7981 gacgttggtg gcggcggccg ccacgggcac cagcttcgcg ctttccgccg cgccattggc
     8041 caacagcgtg ccgccactgg tcagcgtcac ggaggtgttg gacagcgcca cgccgtcgat
     8101 ttctaaaatg ccgccgttga ccgatccgac gttggcgttg gccgacccca gcgcgccggc
     8161 gttggtcacc gccagcgtgc cctggcttac cgtcacgccg cccgggatcg tgttcgcgcc
     8221 gtcaaggatc cagcggccgg cgccggtttt ggtaacggaa ctggagacgc cgccggtgtt
     8281 ggaaatgctg atgctgtgga cgacgttgtt ccccgtgttg gcgccaccga gtgtccacgc
     8341 cgtgccgcgc gtcgagttca gcgagttggt gatgctggcg atcgtcagcg tcgcgctgct
     8401 ggtcgtcgaa tcattggtga tcgaacccac gccgtttgtg ctcgacggca ggtgaagcga
     8461 aatcggcgcc tgcaccacct ccgggttcac caccgtcgcg gtcatgttga tgccgaggtt
     8521 gttgccgagc tgaaggttcg gcgaaccgct ggccgcgccg atgtagtacg cgccggcgtt
     8581 ggcggtgtcg aaggtcaggc ggccgatcat gcggctgccg ctggaactcg cggtgccgtc
     8641 gatgaggatc gggttgttga tgtctccgat ggtggtccca ccaacgacgc tgagcgcggc
     8701 gttgaacgtc gcaacatccg tcgtgttggt cgagttaccc gttgggttga tgcctggaac
     8761 cgcgccacca gaccagttgg tgacagtggt ccagttggcg tcggtggtgc cgacccaact
     8821 ctggttggcc gccagcgcgc cctgagcgcc caaaaggatc gccgcattgc cggtggcaat
     8881 ggcgagagcg gtctttgctg aggcgagcct gcgccggctc gaccggccat tgcaacccac
     8941 cgtgttcaga cgctcgtgcc gcgcatgtag atcaagggac atttctcgtg cctccgcagg
     9001 atggaaatcg ggaccggctt tgaaccgcca agccagggtc attgccgctt gttgactgac
     9061 cgccacagca tatccgatcg atgattatga ctgtaccatc ttgcagaggt gttgcaagaa
     9121 ataaatcaca aatttccatg gttctgccgg gattgtgtca tggtaattac cgggacagcg
     9181 cgggctgcgc cgccggcgac agcgcgttgg aaccgcacaa acttcggctg caaagcgggc
     9241 taaataggag tggtggccgc gcctaattca gcaaatatcg accacgtccc aggttgtggg
     9301 aattgccacg accccgcaaa gtgagcttgg acttttgaaa ccagactact ttttaattcg
     9361 ggcgggaagt ggatcaccgc acttcgtctg tgacgaccgg atgcatctcg atgtttcggt
     9421 agcacacctc agccccttcc gcctggaaca gaatccgccc cttgcgcaac ggcacccact
     9481 cccccggctt atccaggttt ggcaccgtcg cgtgcaggat gcggttgttg acccggccgt
     9541 tcacgatgtg caccgcgctg tcgccgcgga cgatgatctc gacggtgttc cagccatcca
     9601 cctctgccat tgggtcgcga atgactcggc tattcaccgg catcttctgc gggctggtgt
     9661 actcaatgcc ccccgccgtc gcttccatga acgtcggcgt cttcgccttc gccgggtcga
     9721 tcatcgtggt gaccgacgtt cccaccgcaa agatatcccc cgtgtcctgc tccatcacct
     9781 ggcactcgat gctgaatggc caagtgccgt tcagccaacc atcgggcccg gtcacgtggt
     9841 aaagacaccc cgcgtcacgc cgagacttgc ttcgtccgcc aaagcgcttt gtgccccact
     9901 tgtactggaa ccgcaggtgg taatcggaga actcctcctc ggtggcgaag tagccgaacg
     9961 gcatcgccgc gccgtcggtc gaatccctat agatatgcac gacgccatcg cggtccactt
    10021 ggaagacctt ctgcgggtca ttgttcttcc cgtcgccttt gagatacgtg tagaagccgg
    10081 tgaggtcttt gccgttgaac agcggcttga aattgcccgc gggctggctg gtttgagcgc
    10141 acgctgagct agcaacgaac gcggagcaga tcattgcgaa cgcgaagcca aggcgcgggg
    10201 agggtcgcat ctttatgcta ctgcgcgcgg ccacgcatgt tgatttaatg attagatgaa
    10261 cgcacagacg cagaggccgc ggagataaga aatgcgagga aggagctggc tcaaagcgct
    10321 ctgcttcctc ctctgccttt ctaacctctg cggcctccgc gtctgtgcgt tcatccctac
    10381 gccgcctgcg gcaggaactg cgtcagcgag tccggcagct gcttcaaccc cc
//
LOCUS       DATIMH010000771         5102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5386695, whole genome shotgun sequence.
ACCESSION   DATIMH010000771 DATIMH010000000
VERSION     DATIMH010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5386695"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            686..2029
                     /locus_tag="VLI90_13495"
     CDS             686..2029
                     /locus_tag="VLI90_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HSV15268.1"
                     /translation="MNAQTELAAALEQHRKGQFDPAETAYRTILAANPRDANALHLLG
                     LLLHQRGRSNEGADLIRQAILIQPNVPAFYSNLGEALRAADRLEEAIDAHIRGVRLKP
                     DFYEGIHNLGGAFGQANRVTDAIACYREAIRLRPDDPEPHWNLAVALLVSGDLANGFR
                     EFEWRLQRPLTRGRPYQRPRWIGEQLSGKTLLLWSEQGFGDVIQFSRYLPLVKERSGA
                     RVILDVHPELVDLLKQQQLADEVVPAHGKAPPAFDAHAPLMSVPYILASTLETLPRTV
                     PYLAPPASRVGEWAALLPSDGSKKVGIAWAGRPTHHNDRRRSMPPALLSALGDVKGVT
                     FVTVQPRGATTAPPADLPLLDLGPRLRDFGDTAALLSQLDLLISVDTAVVHLAGALAR
                     PVWTMLPFAPDWRWLLDREDTPWYPTMRLFRQRRVGVWNDVIERVRGELAQFSAT"
     gene            complement(2280..2957)
                     /locus_tag="VLI90_13500"
     CDS             complement(2280..2957)
                     /locus_tag="VLI90_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSV15269.1"
                     /translation="MHRVAKLMIIVAAALLAGGCKNALYDENVQLHQQNRELQAQLDS
                     KNAQLRSAPDPAQMQAMQGEILDRDRKISELQSQLRQPTPVAPGAPPPDPAIAGIETS
                     YDAKAGTMTVNLPGDVLFDVGKADLKASAKTTLNKIAAALKRDYQGKPVRVEGHTDAD
                     PIRATKDQWEDNLDLSLGRAAAVSRYLEQQGVSPKLVTTSGFGEHRPRGNTKAKNRRV
                     EIVVVLK"
     gene            complement(2975..3352)
                     /locus_tag="VLI90_13505"
     CDS             complement(2975..3352)
                     /locus_tag="VLI90_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15270.1"
                     /translation="MNDTWNNIKFWTKITFFGLLGLILIIVLSRNWDTEVPHVDLLFV
                     KYEKPRLLVVLFFTAVGSIFGWWLFWAVLKTFRQVRGARVRGRTEKLERDMAEMKAKA
                     AMLQTKPTAGEGVTVQVDKLGNS"
     gene            complement(3381..4679)
                     /locus_tag="VLI90_13510"
     CDS             complement(3381..4679)
                     /locus_tag="VLI90_13510"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HSV15271.1"
                     /translation="MPLIAYAIFFFLVGACIGSFLNVVVWRLPRGESLITPPSHCPKC
                     DKQLAWFDNVPIVGWLALRGRCRYCKEPISPRYPIVEAITAALFVLYFLAYYLLQMRT
                     CCPQPRGVDVGDQQPMIVHVGLWILGESWPIYVLYMITIAGLLAASLIDAELFIIPTQ
                     IPYVIAGLAFLVHAIADRPWIPGSLNLVGGFGATAAALSAGAAVGLLISLALWWLKIL
                     PTSFPQGEPLLDVDRAAIEEEIAEAKKRGEHIEYEPLPPPMAKAEILGEIRKEILFLM
                     PPLALGAAWMTLTMVVPTLRDAWANQMRYDWLTGLLGSLLGALVGAFVVWLTRILGTL
                     AFRRVAMGLGDVHLMFGVGAVIGAAGATIAFFIAPFFGILIAIYMMITRKGREMPLGP
                     YLSLATAFVMLFYCPIAAYLTPGLQGMLLVLGGLLGHHGG"
     gene            complement(4756..>5102)
                     /locus_tag="VLI90_13515"
     CDS             complement(4756..>5102)
                     /locus_tag="VLI90_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HSV15272.1"
                     /translation="GEMELAQLKDHVLALGGGAVLREENRLAITGGGHTIVYLRADPA
                     ELHRRVHADAATAANRPALTKLGGGIEEIRSVLAAREPIYRQVMTAAVDVTAKSPEAV
                     VEEIVQLLNVVR"
BASE COUNT          936 a         1620 c         1637 g          909 t
ORIGIN      
        1 tagcgtcgat cgttttcgta gccggggacg gaagcccggt cttttgtgcc tcgagacgga
       61 ccggggcttc cgcccccggc tattatagat gagcgcgcaa catcaaccgg cggcgcaact
      121 tcgcgccacc ggttggactg ccatggtctt tggtcaactc gacggcgcgc actcaccaat
      181 gcgagcgcac gaccagccgc gccgcccgac ccacccaaat tttttgactg ccacggaacg
      241 ggcgtgcctc acgccatcgc tacccctacc cacgctgaaa gagcaagcag cgcaagcagg
      301 ccggcggcaa tgagcgtatc acgcttgtag tgatgctgat gatgaaccat agggcgatct
      361 ccgatagaag tgactctcgg tcctctgtac ctcgagtgga gcccgcgcgg tcgccaaagg
      421 tgtgaatggg tggctgcgcg gggcgctcgt ccgttgagaa tctttatcgc atcttgacaa
      481 agtttttgaa agcaaaatcc gggggatatc ctattgacag gacgcgcgcc atgtctccat
      541 gcgttagcct cggccgcgca agatctgggg gcacaagcat ctaacacaat ctcaacttcg
      601 tgcgttcttt cacaaggatc tgaaaaattt ttcgtgacag cgcgcgcggc agatgccggg
      661 aagacctcga tatgatgccg ctcgcgtgaa cgcgcagact gaattagccg cggcattgga
      721 gcagcaccgc aagggtcaat tcgaccccgc ggagacggcg taccgcacca tccttgcggc
      781 caatccgcgc gatgcaaacg ccctgcacct gctgggcctg ctgctgcacc agcgcgggcg
      841 tagcaacgag ggggccgacc tgatccggca ggcgatcctg atccagccaa acgtcccggc
      901 gttttactcg aacctcggcg aggccctgcg ggcggccgac cggctcgaag aagcgatcga
      961 cgcccacatt cgcggtgttc ggttgaagcc ggatttttat gagggcatcc acaacctggg
     1021 cggcgcgttc gggcaggcga atcgagtaac tgatgccatc gcgtgttatc gcgaagcgat
     1081 ccgcctgcgg ccggacgacc ccgagccgca ctggaacctg gcggtggcgc tgctggtgtc
     1141 gggcgatttg gctaacggtt ttcgcgagtt cgaatggcgc ctccagcgcc cactcacgcg
     1201 cggtcggccg taccagcggc cgcgatggat tggcgagcaa ctcagcggca agacgctgct
     1261 gctttggagc gagcaagggt tcggcgacgt gatccagttc tcgcggtacc taccgctcgt
     1321 gaaggagcga tcgggggcgc gcgtcattct cgatgtgcat cccgagctgg tcgatctgct
     1381 gaagcagcag cagctggcgg acgaggtggt gccggcgcat gggaaggcgc cgccggcgtt
     1441 tgatgcgcac gcgccgctga tgagcgtgcc atacatcctt gcctcgacgc tggagacatt
     1501 gccgcggacg gtcccgtacc tcgcgccgcc cgcgtcgcgc gtcggcgaat gggcggcgct
     1561 gttgccgagc gacggctcga agaaggtggg cattgcctgg gcgggccgac cgacgcatca
     1621 caacgaccgc cgacggtcga tgccgccggc gctattgtcg gcgctgggcg atgtgaaggg
     1681 ggtcacgttc gttacggtgc aaccgcgcgg cgcgacgacc gcgccgccgg cggacttgcc
     1741 gctgctggac ctgggcccgc ggctgcgcga cttcggtgac accgcggcgc tgttgtcgca
     1801 actcgacctg ctgatcagcg tcgataccgc ggtggtgcat ctggccgggg cgctggcccg
     1861 gccggtgtgg acgatgctgc cgttcgcgcc ggactggcgc tggctgctcg atcgcgaaga
     1921 cacgccgtgg tacccgacaa tgcggctgtt ccgccaacgg cgggttgggg tttggaacga
     1981 tgttatcgaa cgcgtgcgag gggagttggc gcagttctca gcgacgtgaa gcgccgaaac
     2041 aagccccgga tgtacatcct gatgacccga cactttcgca gaagtggcgc gggagctggc
     2101 ccacgccggg ttcggaggta ctccgacgac ccggattctt cagttcagcg cgagaagaga
     2161 tccgggtcgt cggagtacct ccaaacccgg cgtggcgaac ttcgacacgc gccgcagaac
     2221 ggtgtggaaa actgtcgggt cgtcatgtgt gcgcgccgaa gtgtgatcgc gcgtgcgcat
     2281 tacttcaaca caacgacgat ctcgacgcgg cggtttttcg ccttcgtgtt gccgcgcggg
     2341 cggtgttcgc cgaagccgct ggttgtgacc agtttagggc tgacgccttg ctgttcgagg
     2401 taacgcgaga cggccgcggc ccggcctagc gacaaatcga gattgtcttc ccactggtcc
     2461 ttggtcgcgc gaatcggatc ggcgtcggta tggccttcca cgcgcaccgg ttttccttgg
     2521 tagtcgcgtt tcagcgccgc ggcgatcttg tttagcgtgg ttttggccga cgcttttaag
     2581 tcggctttgc cgacgtcgaa cagcacgtcg ccgggcaagt tgacggtcat cgtgccggcc
     2641 ttggcgtcgt acgacgtttc gatgccggcg atggccggat cgggcggcgg cgcgccggga
     2701 gcaaccgggg tgggttgccg aagctgcgat tgcaactcgc tgatctttcg atcgcgatcc
     2761 aggatctcgc cctgcatcgc ctgcatctgc gccgggtccg gagcagagcg cagctgcgcg
     2821 ttcttcgagt ccagctgcgc ctgcagttcg cggttctgct ggtgcagctg aacgttctca
     2881 tcgtacagcg cgttcttgca cccaccggcc aacagcgcag cggcgacgat gatcatcagc
     2941 tttgcgacgc gatgcattcg atcactccga actgttatga gttgccgagc ttgtccacct
     3001 gcaccgtcac accttcaccg gcggtcggct tggtctgcag catcgcggcc ttggctttca
     3061 tctcggccat atcgcgttcc agcttttcgg tgcgcccgcg aacgcgcgca ccgcgtacct
     3121 ggcggaacgt cttgaggacc gcccagaaca accaccagcc gaagatgctg ccgaccgcgg
     3181 taaagaacag cacgaccagc aaccgcggct tctcgtactt cacgaacagc aggtccacat
     3241 gcggcacttc cgtatcccag ttgcgcgaca gcacgatgat taggatcaga cccagaagtc
     3301 cgaagaacgt gatcttggtc cagaacttga tgttgttcca cgtatcgttc attttgtaaa
     3361 acctcgcgcc cgcgcccgga tcagccgccg tgatgtccca acaatccgcc cagcaccaaa
     3421 agcatgccct gcaaaccagg cgtcagatac gcggcgatcg ggcagtagaa tagcatcacg
     3481 aacgccgtcg ccaaactcag gtacgggccc agcggcatct cgcggccctt gcgggtgatc
     3541 atcatataga tcgcgatcag gatgccgaag aacggcgcga tgaagaacgc gatggttgcg
     3601 ccggcggcgc cgatgaccgc gccgacgccg aacatcaggt gcacgtcgcc gagtcccatc
     3661 gccacgcgac ggaacgcgag cgtgccgagg atgcgggtca accagacgac gaacgcgccg
     3721 accagcgcgc ccagcaacga gccgagcagg ccggtgagcc agtcgtagcg catctggttc
     3781 gcccacgcgt cgcgcagagt cgggacaacc atcgtcaacg tcatccacgc agcgcccagc
     3841 gccagcggcg gcatcaggaa gaggatttcc ttgcggattt cgccgaggat ttccgccttc
     3901 gccatcggcg gcggcagggg ttcatattcg atatgctcgc cgcgtttttt cgcctcggcg
     3961 atctcttctt cgatggccgc gcggtccaca tccagcagtg gctcgccctg cgggaaactg
     4021 gtcggcagga ttttcaacca ccacagcgcg aggctgatga gcagcccgac cgccgccccg
     4081 gctgataagg ccgccgcggt ggcgccgaac ccgccgacga gattgagcga gccgggaatc
     4141 cacgggcgat cggcgatcgc gtgtacgagg aacgcgaggc ccgcgatcac atacggaatc
     4201 tgcgtcggga tgatgaacag ctccgcgtcg atcaggctcg ccgccagcag gccagcgatt
     4261 gtgatcatgt agagcacgta gatcggccac gattcgccga gaatccacag cccgacgtgc
     4321 acgatcatcg gctgctgatc gccgacatcc acgccgcgcg gttgcgggca gcaggtgcgc
     4381 atctgcagca ggtagtacgc gaggaagtag agcacgaaca acgccgcggt gatcgcctca
     4441 acgatcggat agcgcggcga gatcggttct ttgcaatagc ggcatcgtcc gcgcagggcg
     4501 agccagccca cgatcggcac gttgtcgaac cacgcgagtt gcttgtcgca cttggggcaa
     4561 tgcgacggcg gcgtgatgag cgattccccg cgcggcagcc gccagaccac gacgttgagg
     4621 aagctgccga tgcaggcgcc gacgaggaag aagaagatgg cgtaggcgat gaggggcaat
     4681 cgaaaagtag cacgggcgtc tcgcccgtgt cttgaaccgc aaggcacggg caagacgccc
     4741 gtgctacgag aggcgttatc ggaccacgtt cagcagctgc acgatctcct caacgacggc
     4801 ttccggcgat ttggcggtca cgtcaaccgc cgcggtcatc acctgccgat aaatcggctc
     4861 gcgcgcggca aggactgaac gaatctcctc gatcccacca cccagcttcg tcagcgccgg
     4921 ccgatttgct gccgtcgcgg catccgcatg aaccctgcgg tgcaattcgg ccgggtccgc
     4981 tcgcaggtaa acgatcgtat gcccaccgcc ggtgatcgcg aggcggttct cctcgcgcag
     5041 gaccgcccca ccgccgagcg cgagcacgtg gtccttcagc tgcgccagct ccatttctcc
     5101 tt
//
LOCUS       DATIMH010000772         4378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5097904, whole genome shotgun sequence.
ACCESSION   DATIMH010000772 DATIMH010000000
VERSION     DATIMH010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5097904"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(391..618)
                     /locus_tag="VLI90_13520"
     CDS             complement(391..618)
                     /locus_tag="VLI90_13520"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HSV15273.1"
                     /translation="MEIPRITHDPAVMGGKPCIRGLRVTVGTILGLLAAGQSRERILQ
                     AYPYLEPQDLDAALAYAAWRMEEREEPLLAS"
     gene            complement(698..1330)
                     /locus_tag="VLI90_13525"
     CDS             complement(698..1330)
                     /locus_tag="VLI90_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15274.1"
                     /translation="MLVAVAIVASVNATARAQRWDFGIAVGYSHIDLDKPGSLFHSKD
                     GPYFDADFAVRLPRLALPLSLGVGITGSGYFDSRTQDFLISSTTLATTTLYSDIGFFE
                     IEPRATLAFYMHQRYRTGLFIKPRIGAGLLIDSYTIDHVTQNSFATIFETHYHSGAAF
                     EVRPAVQVGYSWGPGEVGAEFSYMAAWGDLGDFGSEAQEFRAGPFFTWRF"
     gene            1658..2629
                     /locus_tag="VLI90_13530"
     CDS             1658..2629
                     /locus_tag="VLI90_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSV15275.1"
                     /translation="MPAASPDSISARGAASFIDPPTLMRIKSLHLRARIIVQGFLSGL
                     HRSPYHGFSVEFSEYRQYSPGDDPRYLDWRLFARSDRYYLKRFEEETNVRCHLLVDLS
                     RSMGFSSLAYSKVDYARTVAATLAYFLAMQRDAVGVVTFSDKIADYLPARYRPGHLQR
                     LMVCLERAVAGTSTDLTAPMEQIAATMRKRGLLVLISDLLVDPASLQTRLQYLRSQGH
                     EIIVLRVLDPAEIEFTFDRPATFVDVESGRDLYIDPSVARQQYQRNFQRHADEIARIC
                     RELGVDVHTLVTSQPLELALSAFLQSRLHGKRRAVRSHNRGGGRGGR"
     gene            2862..>4378
                     /locus_tag="VLI90_13535"
     CDS             2862..>4378
                     /locus_tag="VLI90_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HSV15276.1"
                     /translation="MNFLTPLYIAGALAVALPIVFHLIRRTPRNRQPFSSLMFLTESP
                     PRLTRRSRLSDILLLILRGLALVLLALAFARPYFYRKADMNLSASQGKRIAIMVDTSA
                     SMRRGDLWQQATQQVDRVLAEVTPADEVSLFLFGRTVHPAFTFDQWNQLDAAHRAAML
                     RARLAEAKPSWLSTNLGDALATVANQLAESRASEKYADLSRRQIVLVSDVQQGAHVEA
                     LQGHEWPENVLLDVRAVATKQTTNASLQLVKDDPNAPDTAAGKRLRVRVSNQPNSTSD
                     QFAISWANDRGPLAAIELQKVYVAPGRSQVIKIDWPPADAHADRLILSGDDFDFDNTL
                     YVARPPQAIARVAYIGADDGNDPKAMGYYLQSAVAGDSAERKTEFVQRRPDAITDADL
                     IGARLVVVTAAPSKDRVAVIRNFLENSAGAVLWPITDPSAAAPLGRLMGVDSPQIEET
                     SSGDYSLISRIDQDNPIFAAFSDSRFADFTKIHFWKHRRVRFGDGANSPRVIASF"
BASE COUNT          813 a         1417 c         1349 g          799 t
ORIGIN      
        1 tggggatcat catgctctga tgacacggcc gccgcttgcg gcggccccgt gaacacgccg
       61 cgggcgctac agcggcaata cgcgcacgcg taatttcttt tcttcgacaa cgaccagtgc
      121 gccggccaac agcgccgatt catactgccg cagggctgag atgacgagct tcgccatgtg
      181 ttccggcaag atgttttggc cgcgcacttg tatcacgctg gggccactgg cgtgcgtcag
      241 cgcaattgtt ggcgcgtgcc cacgccatga tcaccgcgtc cgcggcgcgg ggatcgccga
      301 agttggacca gtgcagcgcg tcatatccct gactcgacag ttctgcaacc cactctgtgg
      361 aaaggttcat atcgatcagg aggcggatga tcatgatgcg aggagcggtt cctcgcgctc
      421 ttccattcgc caggcggcat acgccagggc tgcgtccagg tcctgcggct cgaggtatgg
      481 atacgcctgg aggatccgtt cgcgtgattg tccggcggcg agcaaaccta ggatggttcc
      541 cacggtcacg cgaaggccgc ggatgcaggg cttgccgccc atgaccgctg ggtcgtgagt
      601 aatccggggg atttccatgg cctgattata tcagcccggc atcgacatgc cacgatctgc
      661 cccgtccggg gcgcagcgcc ttggctccgc gccgtcttca aaaccgccag gtgaagaacg
      721 gccccgcgcg gaactcctgg gcttcggaac cgaagtcacc taagtcgccc caggcggcca
      781 tgtaggaaaa ctcggcgccg acctcgcctg gtccccagga atagccaacc tgcaccgccg
      841 ggcggacttc gaacgccgcc ccgctgtgat agtgcgtttc gaagatcgtc gcgaaagagt
      901 tctgcgtgac gtgatcgatg gtgtaggagt cgatcagcaa tccagcgccg atgcgtggct
      961 tgatgaacaa tcccgtgcgg taccgctggt gcatgtaaaa cgccagcgtc gcgcgcggct
     1021 cgatttcgaa gaagccgatg tccgagtaga gcgtggtggt ggcgagagtc gtcgaggaaa
     1081 tcaggaagtc ctgggtccgc gagtcaaaat agcccgagcc ggtgatgccc acgcccaggg
     1141 acagcggcag ggccaaccgc ggcaggcgaa ccgcaaaatc cgcatcgaag tacggcccat
     1201 ccttgctgtg gaacaggctg cccggcttgt ccaagtcgat gtgcgaatag ccaacggcta
     1261 taccaaagtc ccacctctgc gcccgcgccg tcgcgttcac gctcgcaacg atcgcgacgg
     1321 cgaccaacat cacaacacgg gcaacaccag cgcggcacat agatcctccg atccggcata
     1381 catttgcgtg aagcgtgcgc cagccatctt cgacacctct ggtgatgcga gcaactcaga
     1441 tttcgcaact ggactaacgg accattcccg catcggttgc agattctgcg cgctatacag
     1501 ctcacgattt gaaaacatgc cccgacgccg gtgtctgtgg tactccgaag gcccggaatt
     1561 cggcgcgcaa aagaatccgg gtcttcggag tacctcagac accggcgtcg ggcacgttcc
     1621 tcaccacacc cgtgcgcctc agcggtatac ttaagaaatg cccgccgctt cccccgattc
     1681 gatctccgcc cgcggcgccg catcgttcat cgatccgccc acgctcatgc ggattaaaag
     1741 cctgcacctg cgcgcccgca tcatcgtgca aggcttcctc agcggcctgc atcgcagccc
     1801 ctaccacggc ttctccgtcg agttcagcga ataccgccaa tactcccccg gcgatgaccc
     1861 gcgctatctc gactggcggc tcttcgcccg cagcgatcgc tattacctca agcgattcga
     1921 agaagagacc aacgtccgct gccacctgct ggtggacctg agccggtcga tgggattcag
     1981 ctccctcgcc tattcaaaag tcgactacgc ccgcacggtc gcggcgaccc tcgcatattt
     2041 cctggcgatg cagcgcgacg ccgtcggcgt cgtcacgttc agcgacaaga tcgccgacta
     2101 cctgcccgcg cgctatcgtc ccgggcatct tcagcggctg atggtctgcc tcgaacgcgc
     2161 ggtggccggt acatcgaccg atctgacggc gccgatggag cagatcgccg ccacaatgcg
     2221 caagcgaggc ctgctcgtgc tgatctccga tctgctcgtg gatccggcgt cgctgcaaac
     2281 ccgcctgcaa tatctgcggt cccagggcca cgagatcatc gtgctccgcg tgctcgaccc
     2341 ggcggagatc gagttcacct tcgatcgccc cgccacgttc gtggatgttg aatccggccg
     2401 cgacttgtac atcgatccat cggtcgcgcg gcaacagtat cagcggaact ttcagcggca
     2461 cgcggatgag atcgcgcgga tctgccgcga gttgggcgtt gatgtgcata cgctcgtgac
     2521 atcgcagccg ctggagttgg cgctgtcggc gttcctacaa tcgcgcctgc acgggaagcg
     2581 ccgagctgtc cgcagccaca accgcggcgg cgggaggggc gggcgatgaa actggttcga
     2641 ctccgatctc cggccaatgc gaccgcgaag acgcgaagac gcgaaggcag acgcgaagat
     2701 ctgttttgtg taaagccggc gatggcatcg caggttcgcg atgcttccgg tgatgccatc
     2761 gccggccttg cacagagcgg ttttgattgt ccttcgcgtc ggccttcgcg cctttgcgcc
     2821 ttcgcgttcg cattctcctt cctcgaaccg ggaggagaac cgtgaacttc ctcacgccgc
     2881 tctacatcgc cggggcgctg gcggttgcgt tgccgatcgt gtttcacctg atccgccgca
     2941 caccgcggaa tcgccagccg ttcagctcac tgatgttcct cactgaatcg cccccgcgac
     3001 tcacccggcg gagccgattg agcgacatcc tgttgctgat cctgcgcggg ctggcgctgg
     3061 tcctgctcgc gcttgcgttc gcccggccat atttctatcg caaagcggac atgaatctca
     3121 gcgcttcgca gggcaagcgc atcgccatca tggtcgacac cagcgccagc atgcgccgcg
     3181 gcgatctttg gcaacaggcg acacagcaag tcgatcgcgt gctggccgag gtcacaccgg
     3241 cggacgaagt ctcgctgttt ttgttcggcc gcacggtgca cccggcgttt acgttcgatc
     3301 agtggaacca gctggacgca gcgcaccgcg cggcgatgct gcgggcgcgg ctggccgagg
     3361 cgaagccgtc ttggttgtcg acgaacttgg gcgacgcgct ggcgacggtg gcgaatcaat
     3421 tggccgaatc gcgggcgtca gaaaaatacg ccgacctgtc gcgccggcag atcgtgctgg
     3481 tgtcggacgt gcagcagggt gcccatgtcg aagcgctgca ggggcacgag tggccggaga
     3541 atgtactgct ggacgtgcgg gccgtcgcga caaagcagac gaccaacgcg agcctgcaac
     3601 tggtgaagga cgatccgaac gcccccgaca ccgccgcggg caagcggctg cgcgtgcgcg
     3661 tgagcaatca gccaaattca acgagcgacc aattcgccat cagctgggcg aacgatcgcg
     3721 gcccgctggc ggcgatcgag ctgcaaaaag tctacgttgc ccccggtcga agccaagtca
     3781 tcaagatcga ctggcccccg gccgacgcgc atgcggatcg gttgatcctc agcggcgacg
     3841 attttgattt cgacaacacg ctgtacgtag cccggccacc gcaggcgatc gcgcgcgtcg
     3901 cctacatcgg cgcggacgat ggcaacgacc cgaaggcgat gggctattac ctgcaaagcg
     3961 ccgtcgcggg cgattcagcg gagcgcaaga ccgagttcgt ccaacggcgg ccggatgcga
     4021 ttacggatgc cgacttgatc ggcgctcgtc tggtcgtcgt caccgcggcg ccgtcgaagg
     4081 accgcgttgc agtgattcgc aactttttag agaacagcgc cggcgcggtg ctgtggccga
     4141 tcacggatcc gtcggcggct gcgccgctgg gccgcttgat gggcgtcgat tcgccgcaga
     4201 tcgaagaaac gtcgagcggc gattactcgc tgatcagccg aatcgatcag gataacccca
     4261 tcttcgccgc gttcagcgat tcgcggttcg cggatttcac gaagatccat ttttggaaac
     4321 atcgccgggt gcggttcggc gatggcgcaa attcgccacg ggtgatcgca tcgttcga
//
LOCUS       DATIMH010000773         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2204664, whole genome shotgun sequence.
ACCESSION   DATIMH010000773 DATIMH010000000
VERSION     DATIMH010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204664"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..1652)
                     /locus_tag="VLI90_13540"
     CDS             complement(366..1652)
                     /locus_tag="VLI90_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSV15277.1"
                     /translation="MNQADTAWMLISTALVLLMTPALAFFYGGLVRSKNSLNTMMMSF
                     ISLGFVAVLWALVGYSLAFSTGNNWIGDLSNVFLKGVGLDVRPTTTIPHALFMSYQGT
                     FAIITAALISGAIVERMRFSAYVTFISLWAVVVYSPIAHWVWGGGWLAKMGALDFAGG
                     TVVHVNAAVAAFAAAIVVGQRSDYKKASILPHNVPFVLLGAGLLWFGWFGFNAGSALA
                     AGPIAALAFVTTMLAPAATLVVWTIIDVLRTGKPTSVGAATAIVVGLVAVTPAAGFVS
                     PMSALALGGLAAFPSYFAIQFRSKTSLDDSLDVVAAHGVGGTVGALLTGVFAEKALNG
                     VADGVLFGNPRQLLIQGAAVLAAIVYSGTASFLLLKAIALVMPLRATTEEEASGLDIS
                     LHGEDAYLHDGGMDRINPVETGVFQPATAKSAVSPV"
     gene            1874..>2207
                     /locus_tag="VLI90_13545"
     CDS             1874..>2207
                     /locus_tag="VLI90_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15278.1"
                     /translation="MNQTRSWRRVLTFLDREVETGNKNVALLIAGLPRGRPSIIGADP
                     SARAIGTRMLGSSSNKHFCPPSCLSGSSKLRSPGREKEDAMKMFAALFIGLIAADVSG
                     TWTLHYDQD"
BASE COUNT          470 a          686 c          676 g          375 t
ORIGIN      
        1 cgcctcagag gacggcctca tccacctgct ccagtacggc aaaggccaga tgagtgttcg
       61 catcaagcca tgtgcgtctc cgcacgtcgc gaagtcgcat ttcacatgtc tgggctagtg
      121 ttctgctgtg attggccgtg aacgaggtct cacgtggcgt agaaactggt gaagaacaga
      181 acgaatcgat aggcggtcac gaccgccata gtccagccca agagaagaac tgttttcgac
      241 gtcgttcgcc aggctgattc accatacatt cggcgcgacg gcgccggcag gtcacatgcg
      301 agcctgacct ggcgggcaaa accgccggcc gtccaccgaa tcggcgaacg gccggcactt
      361 gagcgttaga caggtgagac ggcggacttg gcggtcgcgg gctggaacac cccggtttcc
      421 accgggttga ttctgtccat gcccccgtcg tgcagatacg cgtcttcgcc gtgcagcgag
      481 atgtcgagac cactggcttc ttcctccgtg gtcgcgcgga gcggcatcac gagggcaata
      541 gccttcaaca ggaggaagct ggcggtgccg ctatacacga tcgccgcgag caccgcagca
      601 ccctggatga gaagctgtcg tgggtttccg aacagcacgc cgtcggcaac gccgttcagc
      661 gctttctccg cgaacacccc ggtgagcagt gcgcccaccg tgccgccgac gccgtgcgcg
      721 gccacgacat cgagcgaatc gtccagcgag gtcttcgatc ggaactgaat cgcgaagtag
      781 ctggggaacg cagcgaggcc gcccagcgca agagcgctca tcggcgacac gaaaccggcc
      841 gccggcgtca ccgcgacgag gccgacgacg atcgccgtcg cggcgccgac gctggtcggt
      901 ttgccggtgc gcagcacgtc gatgatcgtc cacaccacca gggtcgcggc gggcgccagc
      961 atcgtggtca caaacgccag cgcggcgatc ggcccggccg ccagcgcgct gccggcattg
     1021 aatccaaacc aaccgaacca caacaggccg gcgccgagca gcacgaacgg aacgttatgc
     1081 ggcaggatcg atgccttctt gtagtccgac cgctgcccga ccacaatcgc cgccgcgaag
     1141 gccgccacgg ccgcattgac gtggacgacg gttccgccgg caaaatcgag cgcgcccatc
     1201 ttcgccagcc atccgccacc ccatacccag tgcgcgatcg ggctgtagac gacgacggcc
     1261 cacagcgaga tgaatgtgac gtacgcggag aagcgcatgc gctcgacgat cgccccggaa
     1321 atcagcgccg ccgtgataat ggcgaacgtc ccttgatacg acatgaacag ggcatgcggg
     1381 attgtggtgg ttgggcgaac atcgagcccg acccctttca gaaacacgtt ggaaaggtcg
     1441 ccaatccagt tattgccggt cgagaatgcc agcgagtagc cgaccagcgc ccacagcacg
     1501 gcgacaaagc cgagcgagat gaagctcatc atcatcgtgt tgagcgagtt cttcgatctg
     1561 accaggccgc cgtagaaaaa tgccagcgcc ggtgtcatca gcagcaccag ggcggtcgaa
     1621 atcagcatcc atgcggtatc agcttgattc atgtagacct caatcgtagc caggccgtgt
     1681 gcatcacggc gcgtttaccg acgaaggccg gagcagattt cgtgcggttc cagtccgccc
     1741 gcccgaccga gcgaaggggt ggcgtgcggc tcgcacggcc ccttcagaag gctcgcgcgc
     1801 ctgaatacca agaaccgcgc cagcggggaa tcaggagagc agcgctttta ctgaacaaaa
     1861 ctgacaaatt tggatgaacc agacgcgtag ttggcgaaga gttctgacgt tccttgatcg
     1921 agaagtcgaa actggcaaca aaaatgtcgc tttattgatc gcgggtttgc ctcgtggccg
     1981 tccgtcgatc atcggcgcgg atccgtcagc tcgcgcgata ggcacaagaa tgttgggaag
     2041 ttcttccaat aaacattttt gtcccccaag ttgcctgtcg ggatcgtcta aactccgatc
     2101 gcctgggcgc gagaaggagg atgccatgaa aatgtttgct gcgttgttca tcggcctgat
     2161 tgccgccgac gtatccggga cgtggacctt gcactacgac caggact
//
LOCUS       DATIMH010000774         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_184298, whole genome shotgun sequence.
ACCESSION   DATIMH010000774 DATIMH010000000
VERSION     DATIMH010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184298"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VLI90_13550"
     CDS             complement(<1..675)
                     /locus_tag="VLI90_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15279.1"
                     /translation="MYSTIVLNSLNDSGPGTLRDAVATAASGDTIDASALSGTIALTS
                     GQLQITTDVSIVGPGADSLMLDGGGLDRLVTVAGGTTVSLAGLTFTHGGNVAAGGAIF
                     SDGALTLDHVIVNANTASATGGGIFENSGSLTIVDSTISNNSIAATADAAGGGVFAQA
                     GTLSITGSTFAGNTAQAAGAAPGASNGFAARGGAIDIETATASITNCTFTGNTAQGGD
                     DVDLAAI"
     gene            970..2280
                     /locus_tag="VLI90_13555"
     CDS             970..2280
                     /locus_tag="VLI90_13555"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HSV15280.1"
                     /translation="MWHGSRKVLLIVPALCACLVALIAAPATFAHDAPAQLPDPQSVP
                     EAWNVLEGCVANVDTLLDSNLLRDIGFQLANTSGPLRLLSAKADENPQSARVRAHVEQ
                     LLAMDGQVLIATRATTDPLAQSRQQWKLYRKTFAELEALYPADTVHSAVYICPMHPRD
                     IHLNADDRCSVCGMSLIRRHLPASQVFERPGEASMTLNVISEPLVVGQRADVRIRIAD
                     RDGSAVTLDQLLEMHTRKIHLLINDASLSDYHHEHPTPTTVAGEYAFSFTPTKPGPYR
                     IWADVVPTATSVQEYLIADVPANTASLPLVDRRSVSSVVVNGRTYQLNFGPEPIHARQ
                     TVVGTITVTGADGQGFTKLEPIMGAFAHIVGFSEDHRTVLHIHPYARDPLGPDDRAGP
                     AFAFKFYAPEPGFYRLYGQVQIDRTSEFAPFGVNVLPAEAPDPK"
     gene            complement(2387..2980)
                     /locus_tag="VLI90_13560"
     CDS             complement(2387..2980)
                     /locus_tag="VLI90_13560"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSV15281.1"
                     /translation="MLIPLIIVAAVLLITAAVAAFAYNRLIALRNRYLNAYAQIDVQA
                     KRRYDLIPALVEASKGYLAHERQTLEGVMSARTAAAAANQVAAATPGDRAAMTSIGGA
                     ESALAGALGRFTAVAEAYPQLKANQQLSQLMDELAATENRISFARQFFNDCVMDYNTA
                     IQSFPTVIVARPFGFAPATFFEVHASDRQTPVVANLR"
BASE COUNT          540 a         1038 c         1011 g          550 t
ORIGIN      
        1 gatcgcggcc agatcgacgt cgtccccgcc ctgcgccgtg ttgccggtga acgtgcagtt
       61 ggtgatgctg gcagttgcag tctcgatgtc gatcgcaccg ccgcgcgcag caaagccgtt
      121 actcgcgccc ggcgccgccc cggcggcctg cgcggtgttg ccggcgaacg tgctgcccgt
      181 gatgctcagc gtccccgcct gcgcaaagac gccgccgccc gcggcgtcgg cggtggccgc
      241 aatcgagttg ttcgaaatcg tcgagtcaac gatcgtcagc gagccggagt tctcgaagat
      301 gccgccaccg gttgcgctgg cggtgttggc gttgacgatg acatgatcga gcgtgagggc
      361 gccgtcggaa aagatcgccc cacccgccgc gacgtttccg ccgtgcgtga acgtgagccc
      421 ggccagcgaa accgtcgtcc cgccggcgac ggtgaccagc cgatccaatc caccgccgtc
      481 gagcatcagc gaatccgcgc cggggccgac gatcgagaca tccgtggtga tctgcagttg
      541 gccggacgtg agcgcgatcg tgccgctgag cgcgctggca tcgatcgtgt cgccgctggc
      601 tgcggtcgcc accgcatcgc gcagcgtgcc aggtccgctg tcgttcaagc tgttcagcac
      661 gatggtgctg tacaaaatgc gctgctcgag cggttcaatg gcgcgacgaa tcgcgcgctc
      721 ccgacgcgcg cgctcggcgt gtgcgcatcg tgtgaccgtt cgtgtagacg aagactccgc
      781 gaagccgcgt tcaaccacag acgtccctcg ctcggtaagt tggatgcgat gaatctaccc
      841 caagattgcc ggcccggtcg gacgattgat cgcgaagcga cagttcccgc ggcggagcgc
      901 gtgaagcgat ccattatcgg acgcagagcc gcgtgacgcc ggcagcgatg ggcgttaaaa
      961 ccagtggaga tgtggcacgg gtcgcgcaaa gtcttgctca tcgtccccgc gctgtgcgcg
     1021 tgcctcgttg cattgattgc cgcgccggca acattcgccc acgacgcgcc ggcgcagttg
     1081 ccggacccgc aatcggtccc tgaagcatgg aatgttttgg aaggatgcgt cgccaacgtc
     1141 gatacgctgc tggactcgaa cctgcttcgc gacatcggtt ttcagctggc gaacacgagc
     1201 ggaccgttgc gtctgctgtc cgccaaggcc gatgagaacc cgcagtcggc gcgagtccgt
     1261 gcgcacgtcg aacaactgct ggccatggac gggcaggtgc ttattgctac gcgcgcgacc
     1321 acggatccac tcgcgcaatc gcgccagcag tggaagctat atcgcaagac cttcgctgaa
     1381 ctcgaagcac tctaccccgc cgacaccgtt cattcggcgg tctacatctg cccgatgcat
     1441 ccgcgtgaca tccacctcaa tgccgacgac cgatgcagcg tgtgcggcat gtcgctgatc
     1501 cgccggcatc tgccggccag ccaggtgttc gaacggccgg gcgaagcgtc gatgacgctg
     1561 aacgtcatca gcgagccgct cgtcgtcggc cagcgcgcgg acgtgcgcat ccgcatcgcc
     1621 gatcgcgacg gttcagcggt cacgctcgat cagctgctgg aaatgcacac gcggaaaatc
     1681 cacctgctca tcaacgacgc gagcctgagc gattaccatc acgagcatcc gacgccaaca
     1741 actgtcgcgg gcgagtacgc cttcagcttc acgccgacca agcccggccc ctatcgcatc
     1801 tgggccgacg tcgtccccac cgcgaccagc gtgcaggaat atctgatcgc cgacgtcccc
     1861 gcgaacaccg cgtcgttgcc gctggtggat cgccgcagcg tgtcatcagt tgtcgtgaat
     1921 ggtcgaacgt atcagctgaa ctttggaccg gaaccgatcc acgcgcggca gactgtcgtc
     1981 ggtacgatca ccgtcaccgg cgcagacggc cagggcttca ccaagcttga gccgatcatg
     2041 ggcgcgttcg cccacatcgt tggtttcagc gaggaccatc gcacggtgct gcacatccac
     2101 ccctacgctc gggatccatt aggccccgac gaccgcgccg gcccggcgtt cgccttcaag
     2161 ttctacgcgc ccgagccagg cttctaccgc ctgtatggac aagtgcagat cgaccgtacg
     2221 tcagagtttg cccccttcgg cgtcaacgtc ctgccggcag aagcgcccga tccaaagtga
     2281 taacgaacat cgtttagccg ccgcggcctc gcggcgctct tctacgtccg cgccgcgagc
     2341 ccgcggcggc taaaccatgg cgcattcgcg cggtcgcgcg atgcgattag cgcaaattcg
     2401 ccaccaccgg tgtctggcgg tcgctggcgt gcacttcgaa gaacgtcgcg ggcgcgaagc
     2461 caaacgggcg cgcgacgatc accgtgggga acgactggat cgccgtgttg tagtccatca
     2521 cgcagtcgtt gaagaattgc cgggcgaacg agatgcggtt ctcggtggcg gccagttcgt
     2581 ccatcaattg gctcaattgc tggttcgcct tcagctgcgg gtatgcctcg gcgacggcgg
     2641 tgaaccggcc cagcgccccg gccagcgccg actcggctcc gccgatgctg gtcatcgcgg
     2701 cgcgatcgcc cggcgtggcg gccgccacct ggttcgccgc cgcggcggcg gtgcgcgcgc
     2761 tcatgacgcc ttccagcgtc tggcgttcat gggcgaggta ccctttggat gcttcgacca
     2821 gcgcggggat caggtcgtag cgccgcttgg cctgcacgtc gatctgcgcg tacgcgttga
     2881 gatagcggtt ccgcagggca atgaggcggt tgtacgcgaa cgccgcaaca gcggcggtga
     2941 tgagcaagac ggcggcgacg atgatgagcg ggatgagcat cgcggtgcaa aagctattgc
     3001 ggcgtgcgct gcgccgcaag tgagaagttc acggtgggat tggatgaccg gcgtggcgtt
     3061 ttttccttag acgagtacat acctgccacg ccgggctcgg agcagcctgc cctgagcctg
     3121 tcgaagggta ctccgacga
//
LOCUS       DATIMH010000775         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1631430, whole genome shotgun sequence.
ACCESSION   DATIMH010000775 DATIMH010000000
VERSION     DATIMH010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631430"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VLI90_13565"
     CDS             complement(<1..1074)
                     /locus_tag="VLI90_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSV15282.1"
                     /translation="MSTSLTVLREGTESSRFHRLEHDDARTAIPALDAGAIERELKQR
                     IDGEVRFDKGTRAIYSTDGSNYRQVPIGVVIPRHISDVETTIELARRHGAPILSRGGG
                     TSLAGQCCNVAIVMDFSKYMHRVLEIDPQRRLARVEPGCVLDDLRDTATKQHGLTFAP
                     DPETHNHCTLGGMLGNNSCGVHSLMARNNGMGLRMSDNTQEMEILMYEGHRFRVGPTS
                     EDELDRIIRAGGPQGEIYGKLRAFRNKYADAIRTGYPHLERRVSGYNLNDLLPENGCN
                     VARALVGSESTLVTILEATVRLVPAPKARSLVILGYPDIYSGADHVMEILDFNPIGLE
                     GMDHYLFKFVKAKGDENANLALLP"
     gene            complement(1249..2550)
                     /gene="larA"
                     /locus_tag="VLI90_13570"
     CDS             complement(1249..2550)
                     /gene="larA"
                     /locus_tag="VLI90_13570"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HSV15283.1"
                     /translation="MTIDFPYPGYEAIAPFQLPDQNLMGVYSPRAFDEVDEASVIRKG
                     LEQPIGAPRLRDAVRGCKNVLILIDDGTRETPTARLLPPVIDELHAGGLGDNAIQFLQ
                     APGTHRPMKPDELKKKFGPLYGKYKVHEHHYKDESSLHDFGATRDGTRVTANKLLTEF
                     DFVLGVGSIVPHRVKGLSGGAKIAFPGVAGYEIMARNQWEASMHMSETVMGVPENPMR
                     LRMEEAARMAGLRYIVNVVYDIKKRIVGCFSGDVVRAHREGCKCSREVYAVHLPTRAD
                     ILVIDAHSADRDFWQSAKGPYAGTMAVKDGGSMICVAPNPEGVAKNHENLLKIGFRPH
                     AEIVAMVQRGEVDDLVGVAILADVCQIVEKTDCIMVSPGVKPEEAKRLGFRYANSVNE
                     AVKMAFEKQGQNAKAAVIRYGGHALPLVDDEASDRIASTTS"
BASE COUNT          506 a          951 c          907 g          627 t
ORIGIN      
        1 cggcagcagc gcgaggttcg cgttctcgtc gcctttggct ttcacgaact tgaacaggta
       61 atggtccatc ccctccagcc cgatcgggtt gaagtcgagg atctccatca cgtggtccgc
      121 gccgctgtag atgtcgggat aaccaaggat gaccagcgac cgcgccttcg gcgccggcac
      181 cagacgcacg gtcgcttcaa gaatcgtgac gagcgtgctt tccgacccca ccagcgcgcg
      241 ggcgacgttg cagccgttct ccggcagcag gtcgttcagg ttgtaaccac tgacgcgccg
      301 ttccaggtgc ggatatccgg tgcgaatcgc gtcggcgtac ttattgcgga acgcgcgcag
      361 cttgccgtag atctctccct gcgggccgcc ggcgcggatg atgcggtcca gctcgtcctc
      421 gctcgtcggt ccgacgcgga agcgatggcc ttcgtacatg aggatctcca tttcctgcgt
      481 attgtcgctc atgcgcaggc ccatgccgtt gttgcgggcc atcagcgaat gcacgccgca
      541 ggagttgttg cccagcatcc cgccgagcgt gcagtggttg tgggtttccg gatcgggggc
      601 gaacgtcagg ccatgttgct tcgtcgcggt gtcgcgcaga tcgtccagca cacagcctgg
      661 ttcaacgcgg gccaatcgcc gctgcgggtc gatctccagc acgcggtgca tgtacttcga
      721 aaaatccatg acgatggcga cattgcagca ctgcccggcc aggctcgtcc cgccgccgcg
      781 cgacaggatc ggcgcgccgt gccggcgagc gagctcgatc gtcgtctcca catccgagat
      841 gtgccgcggg atgacgacgc cgattggcac ctggcgatag ttcgagccgt cggtggagta
      901 gatcgcgcgc gtgcctttgt cgaagcggac ttcgccgtca atgcgctgct tcagctcgcg
      961 ctcgatcgcg cccgcatcga gggccggaat tgccgtgcgg gcgtcgtcgt gctcaagtcg
     1021 gtggaagcga ctcgattccg ttccttcccg caaaaccgtc aagctggttg acatgttcca
     1081 ctcgctcttt ctggggaccg ttcgctttca ccacagaggc acggttacgt cgaaaacaaa
     1141 tgcgattgga accacagatg cacacggatt cacacacagc ttcaaacggt ctgctttatc
     1201 tgtgtgcatc tgtgtgcatc tgtgtgcatc tgtggttcct tccacttttc aactcgtcgt
     1261 gctggcgatg cgatcgctcg cttcgtcatc caccagcggc agcgcgtgcc cgccgtagcg
     1321 gatcaccgcg gccttggcgt tctggccttg cttctcgaac gccatcttga ccgcttcgtt
     1381 gacgctgttg gcgtagcgga agccgagccg cttcgcctct tccggtttca cgccgggcga
     1441 aaccatgatg cagtcggtct tctcgacgat ctggcagacg tcggccagga tggccacgcc
     1501 taccaggtcg tccacttcgc cgcgctgcac catcgcaaca atctccgcgt gcggccgaaa
     1561 tccgatcttc aataagttct cgtgattctt cgccacgcct tctggattag gagcgacgca
     1621 aatcatggac ccgccatcct tcaccgccat tgtgccggcg tacggtccct tggccgattg
     1681 ccagaagtcg cggtcggcgg aatgcgcgtc gatgacgagg atgtcggcgc gcgtcggcag
     1741 gtgaaccgca tagacctcgc gcgaacattt gcagccttcg cggtgcgcgc gcacgacgtc
     1801 gccgctgaag cagccgacga ttcttttctt gatgtcgtaa acaacgttga cgatgtaccg
     1861 cagccccgcc atgcgggccg cttcttccat ccgcagccgc attggatttt ccggcacgcc
     1921 catgaccgtt tcggacatgt gcatggatgc ttcccattgg ttgcgggcca tgatttcgta
     1981 gcccgccacg cctgggaacg cgatcttcgc gccgccggac aatcccttca cgcggtgggg
     2041 aacgatggag ccgacgccga ggacgaagtc gaactccgtg agcagcttgt tggctgtgac
     2101 gcgcgtgcca tcgcgcgtcg cgccgaagtc gtgcaggctc gattcgtcct tgtagtggtg
     2161 ctcgtgcacc ttgtatttgc cgtagagcgg gccgaacttc ttcttcaatt cgtccggttt
     2221 catcggccgg tgcgtgcccg gggcctgcag gaactgaatg gcgttgtcac ccagcccgcc
     2281 ggcgtgcagt tcgtcgatca ccggcggcaa cagccgagcc gtcggcgtct cccgcgtgcc
     2341 gtcgtcgatc aggatcagga cgttcttgca gccgcgcacc gcgtcgcgca ggcgcggcgc
     2401 cccgatcggc tgttccaggc ctttgcggat cacgctggcc tcgtccacct cgtcaaacgc
     2461 gcgaggtgag tagacgccca tcaaattctg atccggcagc tggaacggtg cgatcgcttc
     2521 atatccgggg taggggaaat caatggtcat gtgaaccttc ccatccgtga gccgcctctc
     2581 acgtccgtta tttgttgaga gtcaggtaaa tattgtcctc tgccgcttgc ggcttagcag
     2641 atcaagtgtg gcgggtttgc ccaggagacg accagtcggg cccgcttgat ctgctaagcc
     2701 gcaagcggct ggggtggcgg acaagattta tctgaccgtt aatacccacg aataccggcg
     2761 aggcgagttt cgcgcgaatg gataaaattg gatcggcgcc cataccgtat ttcctgactc
     2821 gcaccacttc gtgaccggcg gttactaatg atgggaaatg cggccggcgc tgaacgcacg
     2881 cgtttgccat gagcagccgc agcaataagg cgcccgcggc cgcgcgcaca cgcgcacacg
     2941 agaggcagga tgctcggtat gaaaccctct ccgcccgccg ccgctaagca t
//
LOCUS       DATIMH010000776         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_765120, whole genome shotgun sequence.
ACCESSION   DATIMH010000776 DATIMH010000000
VERSION     DATIMH010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765120"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VLI90_13575"
     CDS             <1..252
                     /locus_tag="VLI90_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15284.1"
                     /translation="SPTPLDPAPIAIDNNPPSSSNDPQPIAASPTATPPVVLASPRPP
                     FIPPPGGPLVGSTPSIPEPCGIFLGVITPLLLQRPRRSV"
     gene            368..>1546
                     /locus_tag="VLI90_13580"
     CDS             368..>1546
                     /locus_tag="VLI90_13580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSV15285.1"
                     /translation="MSPRRLYMDNAATSFPKPRAVTDAMVRYATELGASAGRGAYAEA
                     METGGLIADCRRRLNRLFHGQDPNHFIFTLNCSDALNLAIKGLIDPAEPINHAICTHI
                     DHNSILRPLNALADQGAIEQTRVAVDSKTGLVDPDGVRKSIRRDTRLIAITHVSNVTG
                     TVQPIREIGRIARKHGVPFIVDAAQSAGHLPIDVQADNIDLLAAPGHKGLLGPLGTGF
                     LYIRPGIESQLRSIREGGTGSVSENDRQPDFLPDKYEPGSHNAIGIVGLSEGVKWIAE
                     QTIDKLAAHDLDLVRTFIDGVSDVEGLTYFGPQGVRDRVGVFSVRIDGYDPHELSAIL
                     ESQYGILTRSGIHCAPLAHAAIGTSETGGATRFSFGPFLSKQDVKYATDALAEIASSF
                     I"
BASE COUNT          278 a          578 c          426 g          264 t
ORIGIN      
        1 agcccgacgc cgctggatcc agcgcccatt gcgatcgaca acaacccgcc gtcgtcgtcg
       61 aacgatccgc aaccaatcgc ggcatcgccc accgcaacac cgccggtcgt cctcgcgtcg
      121 ccgcgcccgc cgttcatccc gccgccgggc ggcccgctgg tggggtcgac cccgtcaatc
      181 ccggaaccct gcggcatatt cctcggcgtc atcaccccat tacttctcca acgcccgcgc
      241 cgatccgttt gaaaagccca ccacgccggg ttcggaggta ctccgacgac ccggattttt
      301 cgcgcccccg tgtcccgctt ccacgtcccc atttgatccc ccccgcattt gtcctaatat
      361 tcgcttgatg tctccccgtc gtttgtacat ggacaatgcc gccaccagct ttcccaagcc
      421 gcgggcggtc accgatgcga tggttcgtta cgccaccgag ctgggcgcca gcgccggtcg
      481 cggcgcgtac gccgaggcga tggaaaccgg cgggctgatc gccgattgcc gccggcgctt
      541 gaaccgcctg ttccacggac aggacccgaa ccatttcatc ttcacgttga actgctccga
      601 tgcgctgaac ctggcgatca aaggtttgat cgacccggcc gagccgatca accatgcgat
      661 ctgcacgcat atcgatcaca actcgatcct gcggccgctg aacgctcttg ccgatcaagg
      721 cgcgatcgag cagacgcgcg ttgccgtcga ttccaaaacc ggattagtcg atcccgacgg
      781 cgtccgcaaa tccatccggc gcgacacgcg gctcatcgcg atcacgcacg tcagcaacgt
      841 gaccggcacc gttcagccca tccgcgagat cggccgcatc gcgcgcaagc acggtgttcc
      901 gttcatcgtc gatgccgccc aatccgccgg gcacctgccg atcgacgtgc aggcggacaa
      961 catcgatctg ctcgccgccc ccggacacaa gggtttgctc ggcccgctcg gcactgggtt
     1021 tctctacatc cgtcccggca tcgaatcgca attgcgctcg atccgcgagg gcggcaccgg
     1081 cagcgtcagc gagaacgacc gccagccgga ttttctgccc gacaaatacg agcccggcag
     1141 ccacaacgcg atcggcatcg tcggcctgag cgaaggcgtg aaatggatcg ccgagcagac
     1201 gatcgacaaa ctcgccgccc acgatctcga tctcgtccgc accttcatcg acggcgtgag
     1261 cgacgtcgaa ggcctcacct acttcggccc ccaaggcgtt cgcgaccgcg tcggtgtctt
     1321 cagcgtccgc atcgacggct acgatccgca cgagctctcc gccattttag aaagccaata
     1381 cggcatcctc acccgcagcg gcatccactg cgccccgctg gcgcacgccg cgatcggcac
     1441 cagtgaaacc ggcggcgcga cgcgcttcag cttcgggccg ttcctgagca agcaggacgt
     1501 gaagtacgcg accgatgcgc tggcggagat tgcctcgtcc ttcatc
//
LOCUS       DATIMH010000777         6850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5098506, whole genome shotgun sequence.
ACCESSION   DATIMH010000777 DATIMH010000000
VERSION     DATIMH010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5098506"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /locus_tag="VLI90_13585"
     CDS             complement(<1..1791)
                     /locus_tag="VLI90_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSV15286.1"
                     /translation="MRSTGRHAALVVVLGATVFVFAGGAAGMPASRPSSRPASSQPST
                     MPVGDGAANDLTSLSLEDLMNVEVTSVARQKQRVGEAAAAITVITQEDIQRSGLTSVP
                     ELLRLVPGMDVARLNASTWAISSRGFNDAFANKLLVLSDGRSIYSPLFAGVFWDMHQP
                     LLQDIEQIEVIRGPGATIWGSNAVNGVINIQSKSARDTQGLLVTGRGGNYEQSGAARF
                     GGQIDDKTFFRFTGRYQNTDDFPTTAGPDGHDGWHAIGGAFRLDRYATDKDTFTVQGD
                     LTSTREAETLLLGSFTPPTFMAPTTNSFNALGIDVLGRWTHVFSDQSDLALQVYYDHL
                     TRPVGGSPYHIDTGDIDFHHRFPLGDRQEIVWGLGYRYWADEFKPGPAASMNPQNRDD
                     YIANAYIQDDLTIVKDRLHLIGGTKIEENTYSGFEVQPSGRMVWTPNEQNTLWGAVSR
                     AQRTPARWEQDGRIVSAPSASPLLPVPVQPVVHGTREFDSEGMNAFEVGYRLKPAPKF
                     SLDTAIFYNAYDHLRGFEPGMPGFSATPVPHAVEPVIVANSFYAESYGAEIAANWQVS
                     ERWRLAASYSFLNIRAYSHESFAANPANQQV"
     gene            complement(2077..2508)
                     /locus_tag="VLI90_13590"
     CDS             complement(2077..2508)
                     /locus_tag="VLI90_13590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV15287.1"
                     /translation="MAAATVRGEIIGHQIITSSGGGVLGYRDLGNGQTEVRFTLPGDA
                     ALDGTVDVGDLGALATAYGATAGGAWSQGDFNYDGKVDVGDLGALASHYGQSLGANSG
                     NSSAADPMAITAAAVPEPTSLAMLAMTATSLTLRRWRRHGR"
     gene            complement(2766..5411)
                     /locus_tag="VLI90_13595"
     CDS             complement(2766..5411)
                     /locus_tag="VLI90_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15288.1"
                     /translation="MMLRARRRNAACRLTFATIAAAAALVARAPDVLADPLPAFPGAD
                     GFGANALGGRGGAVYHVTNLTDNVTTPAAGSFRAVLAQIKTDETRSVNPIPAATVVFD
                     VGGTIPLTANLDIKNVNNVTIAGQTAPGPGINMVGFKFDVTSSSGTKANDPTSNIIVR
                     YIASRRGNTISSANDDAIGVLGSTSDPNHNTHDVIMDHVTAAWGMDEGLSVTDSASNV
                     TISNSFITEALQSGHQFGSLIRPRFGASVSYLGNLYANNRSRNPRPGSYNSSTLSFDF
                     RNNTIYNWGDRAGYTGGASETGTETVNMNYIGNYLIAGPSTPSGFNTTSAFIVDTSNS
                     SGTKDDLNISVYQSNNRIDTNRNSIADGTDTGWNAFVRYNGTTATAFAGANDSVITTT
                     PLASPLPFAVSGSSTVDPTTAYQATLASAGAMPWSRNESDARIINQVQNNGGAILLVA
                     PTNEWNDLVNQSAVSRPAGFDTDQDGMADSWEQARGLNPNAADNNAVVTAAESASLAG
                     YTWLEMYLNDLALQANWAGGTSGNWDGILNWNGQLPNLQDSTAAFGNVGAAAQVSVNS
                     IEHVGQLSLDNAAGYTLTGAGGVTMDVLSKSGGTGGYATITVTTGSHTIGVPLALASD
                     THFNIVPADSLLDVTGNLSATGRAITKEGDGSVRLVNVRATALAVNAGGVSIAANVLP
                     NTSAGTSVVQSLAISPGGKLDVTNNSLVIDYTGSIGTLQSDTRQMLFDGRLTSTALAP
                     GQALGYADNATLNRDNFSGQQVDTTSLLMMVALGGDTNLDGKVDVGDLGALATNYGTT
                     AGAFWSQGDSNYDGKVDVGDLGALATNYGTSLVSGSASDAQMASSTATIATSVPEPCS
                     FALIGLGAATLSYRRRRLLPGGFSL"
     gene            5717..6355
                     /locus_tag="VLI90_13600"
     CDS             5717..6355
                     /locus_tag="VLI90_13600"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HSV15289.1"
                     /translation="MKLATVASSCSLKIFSGLILAGGLALSGCSSGEAANGNTPTDNQ
                     TLSNRAQTGLDRFTSVDASLKQVTDNAYAYAVLPEVGEAALGVGGAGGRGVVYRNGQP
                     IGTVTLNLVSVGPQIGGQSYSELVVFQNQSAFDRLQQGGITFGAEASATIVKAGAAAA
                     DRFTKGVAVYILPRGGFEAGASLNGQKLMFQQNQNISQPSGSNNGNPTTQPY"
     gene            complement(6499..6717)
                     /locus_tag="VLI90_13605"
     CDS             complement(6499..6717)
                     /locus_tag="VLI90_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15290.1"
                     /translation="MLLALYHFDYDAGRFISIERLIEEARQEYYENLRLGSVGWHEGT
                     HDLMPWLNYFLVILRRAYLELFERAVEP"
BASE COUNT         1226 a         2162 c         2151 g         1311 t
ORIGIN      
        1 gacctgctgg ttggccgggt tggcggcgaa cgactcgtgc gaataggctc ggatgttcag
       61 gaagctgtac gacgccgcca atcgccaacg ctcgctcacc tgccagttgg cggcgatttc
      121 cgcgccatag ctctcggcgt agaacgagtt ggcgacgatg accggctcca ccgcgtgcgg
      181 cacgggcgtg gcggaaaatc ccggcatgcc gggttcaaat ccacgcaggt ggtcgtaggc
      241 gttgtagaag atggcagtat cgagcgagaa tttcggagcc ggcttcaggc gatagccaac
      301 ctcgaacgcg ttcatgccct cagagtcaaa ctcgcgcgtg ccatgaacga ccggctgcac
      361 cggcactggc agcagcggtg acgcggacgg cgccgacacg atccgcccat cctgttccca
      421 ccgcgccggc gtgcgctggg cgcgcgacac cgcgccccac agcgtgttct gctcgttcgg
      481 cgtccacacc atccgcccgc tcggttgcac ctcgaacccg ctgtacgtgt tctcttcgat
      541 cttcgtcccg ccgatcaggt gcagccggtc cttgacgatc gtcaggtcgt cttgaatgta
      601 tgcgttggcg atgtagtcat cgcgattctg cgggttcatc gaagccgccg ggcccggttt
      661 gaattcatcc gcccagtagc ggtagccaag cccccacacg atttcctgcc gatcgcccag
      721 cggaaagcgg tgatggaaat cgatgtcgcc ggtatcaatg tgataaggac tgccccccac
      781 cggccgggtg aggtggtcgt aatacacctg cagcgccaag tccgactgat cgctgaacac
      841 gtgcgtccac cgcccgagca cgtcgatgcc cagtgcgttg aagctgttcg tcgtcggcgc
      901 catgaacgtc ggcggcgtga atgacccaag cagcagcgtc tcggcttcgc gcgtgctggt
      961 caggtcgcct tggaccgtaa acgtgtcctt gtcagtcgcg taacgatcga gccgaaacgc
     1021 cccgccgatg gcgtgccagc catcgtgccc atcaggcccg gcggtggtcg gaaaatcgtc
     1081 ggtgttctga tagcgcccgg tgaatcggaa gaacgtcttg tcgtcgatct gcccgccgaa
     1141 gcgggcagcg ccggattgct cgtaattccc accccggccg gtgacgagca gcccctgcgt
     1201 atcgcgcgcg ctcttggatt gaatattgat gacaccgttg acggcgttgg atccccagat
     1261 cgtcgcgccg gggccgcgga tcacttcgat ctgctcgatg tcctgcagca gcggctgatg
     1321 catgtcccag aacacaccgg cgaacagcgg tgaataaatc gatcgcccgt cggacaacac
     1381 cagcagcttg ttggcgaacg catcgttgaa cccgcggctg ctgatggccc aagtgctggc
     1441 gttgagccgg gccacatcca tccccggcac aagccgcagc agctccggca cgctggtcag
     1501 cccagagcgc tgaatatctt cctgtgtaat gaccgtgatg gccgccgccg cttcaccgac
     1561 gcgctgcttc tgccgcgcga cgctggtgac ttcaacgttc atcaaatctt cgagactgag
     1621 gctggtgaga tcgtttgccg cgccgtcgcc cacgggcatg gtcgaaggtt ggctgctcgc
     1681 cggtcgcgac gaaggccggc tcgccggcat ccccgccgcg ccgccggcga acacaaacac
     1741 ggttgccccc aacacgacca ccaacgccgc gtggcggccg gtcgatctca cgattggttt
     1801 ttcagaaagc cacataggca acacacccgg cagaacccgg cgaaggttcg gcataaaccg
     1861 atggcgcacg ttattccgac tgtgccggaa ctaacgcata tgccggtttc catatcggat
     1921 taatccgccg ctgcataaac cgcgggcctg cagattccgc gcggcgcggc atcggtccgg
     1981 taatagccgg cgcgcaggtg atgacgcggt gagcgcagcg cgaatcattg aagcttgctt
     2041 ggggcgtgcg cgcgaccaga attgggatga attgggttat cggccgtgac gccgccagcg
     2101 ccgcagcgtg agcgatgtcg cggtcatcgc cagcatggcc agtgaagttg gctccggcac
     2161 ggcagctgcc gtgatcgcca ttgggtctgc ggcgctgctg ttgccgctgt tggcgcccaa
     2221 gctttggccg taatggctgg cgagagcgcc gagatcgcca acgtccacct tgccgtcgta
     2281 attgaagtcc ccttgtgacc acgctccgcc ggcggtggcg ccgtacgcgg tcgccagcgc
     2341 gcccagatcc ccgacgtcca ccgtgccgtc cagcgcggcg tcgccgggca gcgtaaagcg
     2401 cacctcggtt tgcccgttgc ccaggtcgcg gtaaccaagc acaccgccgc cgctggatgt
     2461 gatgatttga tggccgatga tttccccgcg caccgtcgcg gcagccacgc tcgtcagcag
     2521 ctcgttattg gctaagtcga gcttgccgag gttggcgacg ctgagcgtgg atcgttagcc
     2581 tgcagcccac tccatttaca cgtctcggca acaaatcgat caagttgata tggagcttct
     2641 agaaatactt gtagccgcga cgcctgcgtc gcctgtttcg ctcgcaatgc aaggcaacgt
     2701 aggcgtcgcg gctacaaggc gatcgccccg atgtaactca catgtttatc tggaatcccg
     2761 gtcgatcaca acgaaaatcc acccggcagg aggcgccgac gccgataact tagcgtcgct
     2821 gcgccaagcc cgatcagagc gaatgagcat ggctccggca ccgacgtcgc aatcgtcgcg
     2881 gtgctgcttg ccatctgcgc gtcgctggcc gaaccgctaa cgaggctcgt gccataattc
     2941 gtcgccagcg cgcccaggtc gccgacgtcc accttgccgt cgtaattgga atctccctga
     3001 gaccaaaacg cgccggcggt cgtgccgtaa ttcgtcgcca gcgcgcccag gtcgccgacg
     3061 tccactttcc cgtccaggtt cgtgtcgccg ccgagcgcga ccatcatcag caggctcgtt
     3121 gtgtccacct gctgaccgga aaagttatct cgatttaatg tggcgttgtc ggcatagccc
     3181 agggcctggc ccggcgcaag cgctgtgctg gtgagccggc cgtcgaagag catctgccgc
     3241 gtgtcgcttt gcagcgtgcc gatgctgccg gtgtagtcga tcacgagact gttgttggtg
     3301 acatcgagct tgccgccggg agagatcgcc aaggattgca cgacactggt gcccgcgctc
     3361 gtattgggaa gtacattggc ggcgatcgaa acaccgcccg cgtttaccgc gagcgcggtg
     3421 gcgcggacgt tgactaagcg cacgctgcca tcgccttctt tggtgatcgc ccggccggtg
     3481 gcgctgaggt tgccggtcac gtcgagcagg ctgtccgctg gcacgatgtt gaagtgcgtg
     3541 tcgctggcga gcgccagtgg aacgccgatc gtgtgactgc cggtggtgac cgtgatcgtc
     3601 gcgtacccac ccgtgccgcc ggatttgctc agcacatcca tcgtgacgcc accggcgccg
     3661 gtcagcgtgt agcccgccgc gttgtcgagc gagagctggc cgacatgctc gatcgaattc
     3721 acgctgactt gcgccgccgc gccaacgttg ccgaaggcgg cggtcgagtc ctgcagattg
     3781 ggcagctgcc cgttccagtt caaaatgcca tcccagttcc cgctggttcc accggcccaa
     3841 ttggcctgca gcgccaggtc gttcagatac atctcgagcc aggtgtaacc agccagcgat
     3901 gcgctctcgg ccgcggtgac gaccgcgttg ttgtcggcgg cgttcgggtt caacccgcgc
     3961 gcctgctccc acgaatcggc cattccgtcc tggtccgtgt cgaaccccgc ggggcgcgag
     4021 acggcgctct gattgactag atcattccat tcattggtcg gcgctaccag caagatggcg
     4081 ccgccgttat tctgcacctg gttaatgatc cgggcatcgc tttcattgcg gctccacggc
     4141 atggccccgg ccgaggccag cgttgcctga tatgcagtcg tgggatcgac cgtgctggaa
     4201 cccgagacag caaacggcaa cggcgacgcc agcggcgtcg ttgtgatgac cgagtcgttc
     4261 gcccccgcga acgcggtggc ggtggtgccg ttataacgca cgaacgcgtt ccatcccgtg
     4321 tcagtcccgt cagcaatgct gttgcgattc gtgtcgatgc ggttgttcga ctgatacacc
     4381 gagatgttca ggtcgtcctt cgtgccggat gaattagatg tatctacgat gaacgcgctg
     4441 gtcgtgttaa acccgctggg cgtggacggg ccggcaatca ggtaattgcc gatataattc
     4501 atgttgacgg tttcggtgcc ggtttcgctg gcgccaccgg tgtaccccgc gcggtcgccc
     4561 cagttgtaaa tcgtgttatt gcgaaaatcg aagctcagcg tgctgctgtt gtaagacccc
     4621 ggccgcgggt tgcggctgcg attgttggcg tagagattgc caaggtacga gacgctggcg
     4681 ccgaatcgcg ggcggatcaa tgatccgaac tgatgccccg attgcagggc ttcggtgatg
     4741 aagctgttgg agatcgtgac gttgctggcc gagtcggtga cggagagacc ttcatccatg
     4801 ccccacgccg ccgtcacgtg gtccatgatg acgtcgtgcg tgttgtgatt aggatcgctg
     4861 gtgctgccca gcacgccgat ggcgtcatcg ttcgcggacg agatcgtgtt cccccgccgc
     4921 gaggcgatgt agcggacgat gatgttgctg gtcggatcgt tggccttggt gccgctgctg
     4981 ctggtcacgt cgaacttgaa gcccaccatg ttgatgccgg gccccggcgc ggtctgcccg
     5041 gcgatcgtga cgttgttgac gttcttgatg tcgaggttcg ccgtgagcgg gatggtcccg
     5101 ccaacgtcga agaccaccgt ggcggcgggg atcggattaa cgcttcgcgt ctcgtcggtt
     5161 ttgatctgcg cgagcaccgc gcgaaatgag cccgcggcgg gcgtggttac gttgtcagtt
     5221 aagttcgtga cgtgatagac cgccccaccg cggccgccga gcgcgttggc gccaaacccg
     5281 tcggcgccgg gaaacgccgg cagcggatca gccaatacat caggggcgcg cgcgacaagc
     5341 gctgccgcgg cggcgatcgt cgcgaacgtc aaacggcaag ccgcattccg gcggcgggcg
     5401 cgcagcatca tcgattggct ccggttctga attaaggggt ggggcagact catcctccgc
     5461 gggctcaatc ttaagccgtc gccggttcag gatcggttgc agttatggcg agaaacgttg
     5521 catttaggcg tcggactcgc gcgcaggaaa acgtcaacgg gaatgagact tgcggacatt
     5581 ccgtggtgaa acccgcgcga cgcagcggtc gcggcagttc ccgcttttgt gttcacagca
     5641 tttgccgaac ggttcggctc gcgggaatgc ggcctctcgc ggttgccgtc tcgcgcgcat
     5701 ccacctgagg tctaaaatga aactcgcaac tgttgcctcg tcgtgctcgc tgaagatctt
     5761 cagcgggttg atcctagccg gcggcctcgc cctgtccggt tgctccagcg gtgaagcggc
     5821 caatggcaac acgccaaccg acaatcagac gctctccaac cgcgcacaga ccggactgga
     5881 ccgcttcacc agcgtcgatg cgtcgctcaa gcaggtgacc gacaacgcct acgcatacgc
     5941 ggtattgccc gaagtgggcg aagctgccct gggcgtgggt ggcgccggcg gtcgcggtgt
     6001 ggtttaccgt aacggtcaac cgatcggcac cgtcacgttg aacctggtgt cggtgggccc
     6061 gcagatcggc ggtcaaagct acagcgagct ggtagtcttc cagaatcaat cggcattcga
     6121 ccgcctgcag caaggcggca tcaccttcgg ggccgaagcc agcgccacaa tcgttaaagc
     6181 cggcgctgcc gccgccgacc gcttcaccaa gggcgtcgca gtctacatcc tgccgcgcgg
     6241 cggcttcgag gccggcgctt cgctgaacgg ccaaaaactc atgtttcaac agaaccagaa
     6301 tatcagccag ccaagcggca gcaataacgg caacccgacc acgcagccgt actgaaacgg
     6361 caacgggtga ggccgttggc cggaacgttc gattacaaaa tgtaatcagc gcggcacgcg
     6421 cacgcgcgtg ccgccgctgt ttatccgagc gcggtaaaaa tcgcttgagc gaaccaggcc
     6481 cgcgtgccac ctcagtcctc acggctcaac ggcgcgctcg aacagttcga gatacgcccg
     6541 ccgaaggatc acgaggaagt agttcagcca cggcatcaaa tcgtgagtgc cctcatgcca
     6601 tcccactgag ccgagtcgga ggttttcgta atactcctgg cgtgcctctt cgatcagacg
     6661 ctcaatgctg atgaatcggc cggcgtcgta gtcgaagtgg tagagcgcga gcagcatcag
     6721 aaggcgagcg gctcggccgg cacactcgat caacccgcca tggtggagaa ctccagccct
     6781 tttagcttca gcagttcatc ccgtggaact gtcgcgacga ccgagcggcc cggctcgagc
     6841 atgaagtcaa
//
LOCUS       DATIMH010000778         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1053067, whole genome shotgun sequence.
ACCESSION   DATIMH010000778 DATIMH010000000
VERSION     DATIMH010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053067"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1104
                     /locus_tag="VLI90_13610"
     CDS             163..1104
                     /locus_tag="VLI90_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV15291.1"
                     /translation="MDSNTSTARVIDAFWRAMVICPVCREAMADLQQCAHCGGRYGED
                     RGTPILMSADAKRTVTYTFTADRSAPPPERLAKWLNEPPTDTQGELPYHMDAAHAHLL
                     EQLPRGMRVLEIGCGGGQCRPWFQGRGFEYVGVDVSKTRVFDWLQRYGGPDLLCDTHF
                     LPFRDQSFDAVYCAAVTEHLASPQLAAQEIFRVLKPSGHYLGNCSFLEPWHDNSFYHM
                     TPLGAGELLTTAGFEVQNIWPSRGYSGYRSLTAMGFRPPLRVLRHVGQMVYGMYRLQN
                     RAQAALRKLRHRPAVPDIFNRAKVAGATDWIARKPGL"
     gene            1269..>1932
                     /locus_tag="VLI90_13615"
     CDS             1269..>1932
                     /locus_tag="VLI90_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15292.1"
                     /translation="MPLHRTSPFLYGTALAGLICAIPCASSAASVLVSLVRDGSQQGY
                     GDPPWTDMTARLNAAAGAGKIATTSADLNNLNQMLAYDGIWIDLRQFNIGSLTATEAA
                     NLSAYADSGRRIVLIGENSTYATWDSSILSAVGSSYGGGNYTGTTPTTSFVPLTSGVN
                     SVVVVNGGIAGGSDGLRLFTDRVVTVWGPARNILVALDSNMFDDDRLALTGNSTFANN
                     VAS"
BASE COUNT          333 a          600 c          624 g          375 t
ORIGIN      
        1 ggcatttcgc cgggcgtgtc tcggctcgtt cgccagcatc acgccttcgc cgttcactcc
       61 ggcggcggaa aacccgataa agaagtcggt cgcgggcgcg tttcgtcatg cggcgtcgtc
      121 gactatggtt ggccccggca aatttcaatt atgagtcttg tcgtggattc aaatacttcg
      181 accgcacggg tgattgacgc attctggcgg gcgatggtca tctgcccagt gtgtcgcgag
      241 gcgatggcgg accttcagca atgcgcccac tgtggcggcc gctatgggga agaccgcggc
      301 acgccgatct tgatgagcgc cgacgccaag cgaaccgtca cgtacacctt taccgccgat
      361 cgctctgcgc cgccgccgga gcgtctggcg aagtggttga atgaaccgcc gaccgacacg
      421 cagggcgagt tgccgtatca catggatgcg gcccacgcgc atctgctgga gcagttgccg
      481 cgcggcatgc gcgtgctgga gatcggctgc ggcggcgggc agtgccgacc gtggtttcag
      541 ggccgcgggt ttgaatatgt cggtgtggac gtttccaaga cgcgcgtgtt cgattggttg
      601 cagcgctacg gcgggccgga cctgctgtgc gacacgcact tcctgccgtt ccgcgaccaa
      661 tcgttcgacg cggtgtattg cgccgcggtg accgagcacc tggcgtcgcc gcagttggcg
      721 gcgcaggaaa tttttcgcgt gctcaaaccc agcgggcatt acctgggcaa ctgctcgttc
      781 ctggagccgt ggcacgacaa tagcttttat cacatgacac cgctgggcgc gggcgaattg
      841 ctgacgacgg cggggttcga ggtgcagaac atctggccga gccgggggta tagcggatat
      901 cgatcgctga cggcgatggg ttttcgtccg ccgctgcggg tgctgaggca tgtcgggcaa
      961 atggtctacg ggatgtatcg attgcaaaac cgggcacagg cggcgttgcg gaagctgcgg
     1021 catcgcccgg cggtgcccga catcttcaac cgtgccaaag tcgcgggggc gaccgattgg
     1081 atcgcgcgca agcccggact ctaagaggca cgggcaccag atccctccgg cctagccgct
     1141 tcagcggcta ccgtgcagag gatccaggcc acccccgcct aattcgagat ttttattgcc
     1201 gaaccccgcc tgcttgatca aaatcctgcg gctctaggat tctcccgtct tctcgctcgg
     1261 ggtgcatctt gccgctgcat cgcacatcgc catttctata tgggaccgcc ttggcggggt
     1321 tgatctgcgc cattccatgt gcgtcaagcg ccgcctccgt cctcgtcagc ctcgttcgcg
     1381 acggctccca gcagggctac ggcgacccgc cgtggactga tatgacggct cgcctgaacg
     1441 ccgcggcggg cgctgggaag atcgccacaa ccagcgcgga cctgaacaac ctcaatcaga
     1501 tgctcgccta cgacggcatc tggatcgacc tgcgccagtt caacatcggc tcgctcacgg
     1561 cgaccgaagc ggcgaatctc agcgcctatg ccgacagcgg ccgacgcatc gtcttgatcg
     1621 gcgagaacag cacgtacgcg acgtgggaca gctcgatcct gtccgcggtc ggcagcagct
     1681 atggcggcgg gaactacacg ggaacgacgc cgaccaccag cttcgtgccg ctcaccagcg
     1741 gcgtgaactc ggtcgtcgtc gtcaacggcg gcatcgccgg cggcagcgat gggttgcggt
     1801 tgttcactga tcgcgtcgtg acggtgtggg gaccggcgcg gaatattctg gtggccctcg
     1861 actcaaatat gttcgacgat gaccggttgg ctttgactgg aaattccaca ttcgcaaaca
     1921 acgttgcgtc ct
//
LOCUS       DATIMH010000779         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5673843, whole genome shotgun sequence.
ACCESSION   DATIMH010000779 DATIMH010000000
VERSION     DATIMH010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5673843"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1114)
                     /locus_tag="VLI90_13620"
     CDS             complement(62..1114)
                     /locus_tag="VLI90_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSV15293.1"
                     /translation="MRNRITQFVANSDGGTVASVLTPPAPVASNGELLDAYSSAVVGA
                     AQRVSPSVVFLDVQMPVPAGRGPQRPGQGGHGHGSGSGFIFTPDGFILTNSHVVHDAL
                     KIEVTLQDGTKLPARIVGDDPQTDLAVIHITASGLPAVEFGDSSAIQVGQLVVAIGNP
                     YGFQTTVTAGVVSNLARSFRSQTGRMIDNIIQTDAALNPGNSGGPLVDSRGRVVGVNT
                     AIIPMAQGICFAIPANTAQFVAARLIRDGKIRRSYIGFAGQNVPLHRKVVRFHKLDVD
                     TGVLVVGLEPDSPARQAGLREGDVIVSFDGKPIFTIDDIQRLLTAERVGAKLPLTVIR
                     GGTELQTFDVVPLEAV"
     gene            complement(1192..2130)
                     /gene="fmt"
                     /locus_tag="VLI90_13625"
     CDS             complement(1192..2130)
                     /gene="fmt"
                     /locus_tag="VLI90_13625"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSV15294.1"
                     /translation="MPDPLNIIFAGSGEFGAPTLRALVEASDCIVQVYTQPDRPAGRG
                     KKLTPTPIAQLAVELDLPLLRTADINAEALPSADVMVVIAFGQKIGEAAVHHPRLGSV
                     NLHASRLPKYRGAAPINWAMLSGERTTGNSVIRLANKMDAGAILAQSEIPIGDTETAG
                     ELHDRLALDGATLLPRVLVELRDGIAHEREQDHTQATMAPKLSRETASIDWTHDAPTI
                     TRRINGLSPWPGCRVQLVDPAGAAISKLTLLRARALPAAPHDTPGVLQESGVITAGSG
                     AIEILNVQPDGKRPMSLNDYQRGNRWVAGLRVQSVA"
BASE COUNT          351 a          693 c          818 g          427 t
ORIGIN      
        1 tgatcataga caagcccacg gatggctatc cgtgggcttt cggttccttc ctcgcatcac
       61 atcacaccgc ttccagcggg accacgtcga acgtctgtag ctcggtgccg ccgcggatga
      121 ccgtgagcgg cagtttcgcg ccgacgcgct cggcggtcag caggcgctgg atgtcgtcga
      181 tcgtgaagat cggcttaccg tcgaagctca cgatgacgtc gccttcgcgc aggccggctt
      241 gccgcgcggg actgtcgggt tccaagccga ccaccagcac gccggtgtcc acgtccagct
      301 tgtggaagcg caccaccttg cgatgcagcg gcacgttctg cccggcgaag ccgatgtagc
      361 ttcggcggat cttgccgtcg cggatcaatc gcgcggcgac gaactgcgcg gtgttcgccg
      421 ggatggcgaa gcagatgcct tgggccatcg ggatgatcgc ggtgttcaca ccaacgacgc
      481 ggccgcggct gtcaaccagt ggaccaccgc tgttgccggg gttcagcgcg gcgtcggttt
      541 ggatgatgtt gtcaatcatc cggcccgtct gcgagcggaa gctgcgggcg agattgctga
      601 ccacgccggc tgtgacggtc gtttggaagc cgtagggatt cccgatcgcg acgaccagct
      661 ggccgacttg gatggcggac gaatcgccga actccaccgc gggtaggccc gacgcggtga
      721 tgtggatgac ggcgaggtcc gtctgcggat catcgccgac aattcgcgcc ggcagtttcg
      781 tgccgtcctg cagcgtgact tcgatcttca gcgcatcgtg cacgacgtga ctgttggtga
      841 ggatgaagcc gtcgggcgtg aagataaagc cggaccccga gccgtggcca tgccccccct
      901 gtccaggccg ctgcggaccg cggccggcgg gcacgggcat ctgcacgtcg aggaagacga
      961 cggatgggct gacgcgttga gccgcgccga ctacggcgct ggagtaggcg tcgagcagtt
     1021 caccattgct ggcgacgggg gctggcggtg tcaggacgga ggcaacggtg ccgccgtcgg
     1081 agtttgcaac gaattgtgtg attcgatttc gcatcaacca atagtaggcg agggggtcaa
     1141 gcggtcggtg cgtgggggct tccagagcat tcctgcaaac gcgatgcgcc gctacgcgac
     1201 gctttgcacg cgcaggccgg cgacccagcg gttgccgcgt tgatagtcgt tcaacgacat
     1261 cgggcgcttg ccgtcgggtt ggacgttgag gatctcaatc gctccggagc cggcggtgat
     1321 gacgccggac tcttggagaa cgccgggagt atcgtgaggc gcggcgggca gcgctcgggc
     1381 gcgaagcagc gtgagctttg agatcgcagc gccggcggga tctaccaact gcacgcggca
     1441 gcccggccag ggggacaggc cattgatgcg gcgggtgatg gttggggcgt cgtgggtcca
     1501 gtcgatgctc gcggtttcgc gtgagagttt tggcgccatc gtcgcctgcg tgtggtcctg
     1561 ctcgcgctcg tgggcgatgc cgtcgcgcaa ttccaccaac acgcgcggca gcagcgtcgc
     1621 cccgtccagg gccaggcgat cgtgcagttc cccggcggtc tcggtgtcgc cgatgggaat
     1681 ctccgactga gcgaggattg cgccggcgtc catcttgttc gccaggcgga tgacggagtt
     1741 gccggtggtg cgctcgccgc tgagcatcgc ccagttgatc ggggcggctc cgcggtattt
     1801 tggcaagcgc gacgcgtgca ggttgacgct gcccaggcgc gggtgatgca cggcggcttc
     1861 gccgatcttt tgaccgaagg cgatgacgac catgacatcc gccgatggca acgcctcggc
     1921 gttgatgtcc gcggtgcgga ggagcggcag gtctagctcc accgcaagct gcgcgatcgg
     1981 ggttggcgtc agcttcttcc cacgaccggc tgggcggtcg ggctgggtgt agacctgcac
     2041 gatgcagtca cttgcttcaa cgagggcgcg aagcgtgggc gcgccgaact caccactgcc
     2101 ggcgaagatg atgttgagcg ggtccggcat ctttgatagc cgggggcgga agccccggtc
     2161 ttttacgctg caaacggacc ggggcttccg cccccggcta ttaaccatct cgttgcgtta
     2221 gcgcttgctc ttctttttcg gcggcgccgg cggggccggg tggtccgccg cgtattgctc
     2281 ttcgagatc
//
LOCUS       DATIMH010000780         5393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1632604, whole genome shotgun sequence.
ACCESSION   DATIMH010000780 DATIMH010000000
VERSION     DATIMH010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632604"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VLI90_13630"
     CDS             complement(<1..185)
                     /locus_tag="VLI90_13630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV15295.1"
                     /translation="MTAKSEPSTILLAGGGTGGHLYPGLAVAEELRAANPQTNIVFLC
                     TTREIDKVILEPTGFEY"
     gene            complement(182..565)
                     /locus_tag="VLI90_13635"
     CDS             complement(182..565)
                     /locus_tag="VLI90_13635"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV15296.1"
                     /translation="MPGTINTATIDGKRYVLVPEADYKRLKAGTKIVVPERELPAMPR
                     KLPNGNYNAVDYARVSLARKIIVARKEAGLSQAQLARAAGIRPEVLNRIERARVSADT
                     GTVAKIEAGLSAKPARRQSARANPQ"
     gene            complement(653..859)
                     /locus_tag="VLI90_13640"
     CDS             complement(653..859)
                     /locus_tag="VLI90_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15297.1"
                     /translation="MPVEVTPEAAEELDRLPLVIHARVTEIIERLERWPTVPGDNPLV
                     RDRREIFASAPAHIASSSVCRDSG"
     gene            complement(915..2114)
                     /locus_tag="VLI90_13645"
     CDS             complement(915..2114)
                     /locus_tag="VLI90_13645"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HSV15298.1"
                     /translation="MTDSIYRLRHRDILCVCTLSLLCLGVLMVHSATMRVTHNTAWHW
                     SAEGTKQLIFALVSLGVFFALGRMDYTRLATAGSSWRRSPITWLLIAAAAVCVLVLLP
                     IPYLSMQVNGARRWLRLGPIQLQASELAKWATVLFLAYWLSNRPVDLNKFFRGFVPTL
                     IPVGVLCLLVVIQDFGTAALIAVCALTMLIAGRARAWHLLIVIPPALVAGFWFVHHTP
                     YRWRRMTAFIDPYASPDREGYHMIQSLLSFSTGGVTGKGLGNGIQKLGYLPEDTTDFI
                     FAVICEELGLFGAIFTIAMYLGIVFVAWQIIRQKRDSFGCMLAFGVASMLSLQAVINI
                     AVATVSVPTKGLSLPLVSAGGSGLVITAAALGLMYSVACRDPEEAAEWIDAPQFTTPS
                     APARAAA"
     gene            complement(2252..3394)
                     /gene="mraY"
                     /locus_tag="VLI90_13650"
     CDS             complement(2252..3394)
                     /gene="mraY"
                     /locus_tag="VLI90_13650"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HSV15299.1"
                     /translation="MLFLLVQRLHSLLEAHHLGFLRVFNAVEFQATAAMVLSFIICII
                     MGPRTIAWLRQQKIGDNPDFDQADINKMMEGKKGTPTMGGVLIISSILVTTLLLADLS
                     NFYVNMAIVCVLLLGAVGAADDWLKLTVARREGNRQGLTSLEKLLFQLGIAAILSVFT
                     YRYGGDIAETKTLYFPLLKNIAIPLGVGIYVLLGTLVLTGSSNAVNLTDGLDGLASGC
                     MTIASFTFVILAIIVGNRVYANALLIPHIEKAGQMAVVAGAMVGSCLGFLWFNCNPAR
                     VFMGDTGSLALGGLIGYIAMVIRHELLLVFIGGIFVAEALSVMIQVSYFKYTKRKYGA
                     GRRIFLMSPLHHHFQKKGWSENQVVVRFWLISFMLAAMALATIKLR"
     gene            complement(3528..4514)
                     /locus_tag="VLI90_13655"
     CDS             complement(3528..4514)
                     /locus_tag="VLI90_13655"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV15300.1"
                     /translation="MKYRALGRTGFNVSEVSFGAWAIGGAWGDVDDATSLRTLHRAID
                     RGVNFIDTADVYGDGRSERLIAQLKRERPSDTIIVATKAGRRLDPHTAPGYNRANLTA
                     FIERSLKNLQTDALDLVQLHCPPTEVYYEPDVFGVLDDLTQAGKIRNYGVSVEKVEEA
                     LKATELPNVKTVQIIFNLFRHRPSERFFPLAKERKIGILARVPLASGLLTGKINQHTQ
                     FAADDHRNFNRHGEAFDVGETFSGVDFDTALKAVDELRPLVPAGATMAQFALRWILMF
                     DAISCVIPGAKRPEQLDDNVAAAGLPALPAGTMAKANEIYHRLIRPLVHQRW"
     gene            complement(4537..5004)
                     /locus_tag="VLI90_13660"
     CDS             complement(4537..5004)
                     /locus_tag="VLI90_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15301.1"
                     /translation="MTNFEASGDFADWPKNERGKPTQVAVFGMHHLRTGKEFELLWTA
                     QFPDLDKAMYWAAKKELEVIKRLGWSVARRVLWQHDLSPEAAVAAAYARMRPQMESAL
                     RATRDGYDPALAMDSGGDPNAPLKPVDYSKIDGLVVDIDGPCIGSIPNDPRER"
     gene            complement(5057..>5393)
                     /locus_tag="VLI90_13665"
     CDS             complement(5057..>5393)
                     /locus_tag="VLI90_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSV15302.1"
                     /translation="TSVPHNSRLWLKVFLDPTMETAPRQNVVRDEEVIEKGDPGLKVD
                     SVCGIWPGAEWMEREVYDMFGIIFVGHPDLRRILTWNGFGSYPLRKDYPLRGVGERED
                     YKIVTREGA"
BASE COUNT         1013 a         1669 c         1691 g         1020 t
ORIGIN      
        1 atgtattcga aaccggtggg ttcgaggatg actttgtcga tctcgcgcgt cgtgcagagg
       61 aagacgatgt tcgtctgcgg attcgccgcg cggagttcct ccgccaccgc caggcccgga
      121 taaagatgcc cgccagtacc gccgccggcg agaaggatgg tggatggttc ggactttgcg
      181 gtcattgtgg atttgcccgg gcggattgtc gtcgcgcggg ttttgcagaa agaccggctt
      241 caatttttgc gacagtgccg gtgtcggccg aaactcgcgc tcgttcgatt cggttcagca
      301 cctcaggtcg aatgcccgcc gctcgcgcga gctgggcctg agacaatccc gcttcctttc
      361 gcgcgacgat gatcttccgc gccagtgaga cgcgggcgta atcgacagcg ttgtaattgc
      421 cattcggcaa cttcctgggc atcgccggca attcccgctc cggtacgact atcttcgttc
      481 ccgccttcag tcgcttataa tccgcctcgg gaaccagcac atagcgtttg ccatcaatcg
      541 tggcggtgtt gattgtgcca ggcatttcgt ctcccttcgg atgcgccgct gcgccgccgc
      601 cggcgcgcct gtcattgata cacgtctcgc cgattatcga ccttccagct gatcacccgc
      661 tgtccctgca cacggaagac gacgcgatat gggccggtgc ggatgcgaaa atttcccttc
      721 tgtctcgaac caacgggttg tcgccgggca ctgtcggcca ccgctccagc cgttcgatga
      781 tttccgtcac ccgagcatga atgaccagcg gaaggcggtc aagttcttcg gccgcctctg
      841 gggtgacttc aaccggcatg gtcgcagctc aagcttagcg tcgttgactt agatgtcaat
      901 tggcttgact ttccctaagc cgccgcgcgc gccggcgcag acggtgtggt gaactgcggc
      961 gcatcgatcc actccgccgc ttcttccggg tctcggcagg cgacgctgta catcaatccc
     1021 aacgccgccg cggtgatgac caggccgctg ccgccggcgc tgacgagcgg taaggataaa
     1081 cctttggttg gcacgctgac ggttgcgacg gcgatgttga tgacggcctg caggctcagc
     1141 atcgacgcga cgccgaaggc gagcatgcag ccgaagctgt cgcgcttttg gcggatgatc
     1201 tgccaggcga cgaagacgat gccgaggtac atcgcgatcg tgaagatcgc gccgaacagg
     1261 cccagttcct cgcagatgac ggcgaagatg aagtcggtcg tgtcttccgg caggtatccg
     1321 agcttttgga tgccgttgcc taaccctttg ccggtgacgc cgccggtgga gaagctgaga
     1381 aggctttgga tcatgtgata gccttccctg tcgggcgagg cgtagggatc gataaacgca
     1441 gtcatgcgac gccagcggta gggcgtgtgg tggacgaacc agaagccagc gaccagcgcc
     1501 ggcgggatga cgatgagtaa atgccacgcc cgcgcgcggc cggcgattaa catcgtcagc
     1561 gcgcacaccg cgatcaaagc agcggtgccg aaatcctgga tcaccaccag caggcagaga
     1621 actccaaccg ggattaacgt cggcacgaag ccgcggaaga atttgttcag atcgaccggg
     1681 cggttcgaaa gccaatacgc caggaacaac acggtggccc acttcgccaa ctcagatgcc
     1741 tgcaactgga tcggccccaa tcgaagccat cgccgggcgc cattcacctg catgctcagg
     1801 tacggaatcg gcagcagcac cagcacgcac accgcggcgg cggcgatgag cagccaggtg
     1861 atcgggctgc gccgccaact cgaccccgcc gtcgccaagc gcgtgtaatc catccggcca
     1921 agcgcgaaga acacgccgag cgagacgagc gcgaagatca gctgcttggt tccttccgcc
     1981 gaccaatgcc aggcggtgtt gtgcgtgacg cgcatggtgg ccgagtgcac catcagcacg
     2041 cccaggcaca gcagcgagag cgtgcagacg caaagaatat cgcggtggcg caggcggtag
     2101 atggagtcgg tcatctacga ccgttatcgg acgtgagggg tcgggggctt gagagttcgc
     2161 tcgttcatcg tagccggggg cgggagcccc ggttctttct cctgcgaacg gaccggggct
     2221 tccgccgccg gctatgaagg aggtgccgag atcaccgcag cttgatcgtc gccagcgcca
     2281 tcgccgccag catgaaactg atcagccaga accgcaccac cacctggttc tcgctccacc
     2341 cctttttctg aaaatgatga tgcagcggcg acatcaggaa aatccgccgg ccggcgccgt
     2401 attttcgctt ggtgtatttg aagtagctta cctggatcat caccgacaac gcctcggcca
     2461 cgaaaatgcc gccgatgaag accagcagca actcgtggcg gatgaccatc gcgatgtagc
     2521 cgatcaatcc gcccagagcg agtgatccgg tgtcgcccat gaacacgcgc gcggggttgc
     2581 agttgaacca gaggaaaccg aggcaggacc ccaccatcgc cccggcgacg acggccatct
     2641 ggccggcctt ttcgatgtgc gggatgagca gggcattcgc gtacacgcgg ttgccgacga
     2701 tgatcgcgag gatgacgaac gtgaagctgg cgatcgtcat gcacccgctg gccaggccgt
     2761 ccaggccgtc ggtcaggttc actgcgttgc tcgatccggt gagcacgagg gtgccgagca
     2821 ggacgtagat gccgacgccg agcggaatgg cgatgttttt cagcagcggg aagtagagcg
     2881 tcttcgtctc ggcgatgtcg ccaccgtatc gataggtgaa gacggacaag atcgcggcga
     2941 tgccaagttg gaacagcagt ttttccaggc ttgttaagcc ctgacggttc ccttcgcgcc
     3001 gcgcgacggt gagcttgagc caatcgtcgg ccgcgcccac ggcgcccagc agcagcacgc
     3061 agacgatcgc catgttcaca taaaaattcg acaggtccgc tagcagcaaa gtggtgacga
     3121 ggatggacga gatgatcagc acaccgccca tggtgggcgt gcctttcttg ccctccatca
     3181 tcttgttgat gtcggcctgg tcgaagtctg ggttgtcgcc gatcttctgt tggcggagcc
     3241 acgcgatcgt gcgcgggccc atgatgatgc agatgatgaa gctcagcacc atagccgccg
     3301 tggcctggaa ttcgacggcg ttgaagacgc gcaaaaagcc aaggtggtgg gcttcgagaa
     3361 gcgagtgcag acgctggacc aacaggaaga gcatggggaa aacgatgaac gaggagcgac
     3421 aaaaactgaa tagccggggg cggaaggccc ggtcttttca cgttcgaaag gaccgggact
     3481 tccgcccccg gctattaagg gtgatcatcg gtcattttcg cggttcatca ccaacgctga
     3541 tggaccagcg gccggatcaa ccggtggtaa atctcattcg ccttcgccat cgtgcccgcg
     3601 ggcaacgcgg gcagtccggc ggcggcgaca ttatcgtcca gttgctccgg ccgcttggcg
     3661 ccggggatga cgcaggagat cgcgtcgaac atcaggatcc accgcagcgc gaattgcgcc
     3721 atcgtcgccc ctgccggcac gagcgggcga agttcatcga ccgctttgag ggccgtgtcg
     3781 aaatccacgc cgctgaaggt ctcgccgacg tcaaatgctt cgccgtgacg gttgaagttg
     3841 cggtgatcat ctgccgcgaa ttgcgtgtgc tggttgatct ttcccgtgag gaggccgctc
     3901 gccagcggca cgcgcgccag gatgccgatc ttccgctcct tcgccagcgg aaagaaccgc
     3961 tcgctgggcc gatggcggaa gaggttgaag atgatctgca cggtcttgac gttggggagc
     4021 tcggtcgcct tcagtgcctc ttccaccttc tcgacgctca cgccatagtt gcggatcttg
     4081 ccggcctggg tgagatcgtc cagcacgccg aacacgtccg gctcgtaata aacctccgtc
     4141 ggcgggcagt gcagttgcac cagatcgagc gcgtccgtct gaagattctt caagctgcgc
     4201 tcgatgaacg cggtgaggtt ggcgcggttg tagccgggcg cggtgtgggg atcgagccgc
     4261 cggccggcct tggtggcgac gatgatcgtg tcgctcggcc gctcgcgctt cagctgggcg
     4321 atcaaccgtt cgctgcgacc gtctccatag acgtcggcgg tgtcgatgaa gttcaccccg
     4381 cggtcgatcg cgcgatgcag cgtgcgcagt gacgtcgcat cgtccacgtc gccccacgcg
     4441 ccgccgatcg cccaggcgcc gaaggaaact tcagagacgt tgaaaccggt tctgccaagt
     4501 gctcggtact tcattgtggt ggcgcttccg cgaaggttat cgctcgcgcg gatcgtttgg
     4561 gatgctgccg atgcagggcc cgtcgatgtc cacgacgagc ccgtcgatct ttgagtaatc
     4621 caccggctta agcggggcat tcgggtcgcc gccgctgtcc atcgccagcg ctggatcgta
     4681 accgtcgcga gtggctcgca gggcggactc catctgcggg cgcatccgcg catacgccgc
     4741 cgccacggcg gcctcgggcg acaggtcatg ctgccacagc acccgcctgg cgacggacca
     4801 accgagccgc ttgatcactt cgagctcctt cttggccgcc cagtacatcg ccttgtccag
     4861 gtcggggaac tgcgcggtcc aaagcaactc gaattccttt ccggtacgga ggtggtgcat
     4921 gccgaacacc gccacctgcg tgggcttgcc gcgctcgttc ttgggccagt ctgcgaagtc
     4981 gccggaggct tcgaagttgg tcatcgtgaa ctccccggaa ttggcgaaac catggtcgct
     5041 aaagcgacca caccggtcag gctccctcgc gcgtgacgat cttgtaatcc tcgcgctcac
     5101 ctacgccgcg caggggataa tccttccgca gcggatagct gccgaacccg ttccaggtca
     5161 ggatccgccg caggtccgga tgcccgacaa agatgatgcc gaacatgtcg tacacttcac
     5221 gttccatcca ctccgcgcca ggccagatgc cgcagacgct gtccaccttc aaccctgggt
     5281 cgcctttttc gatcacctct tcatcgcgga ccacgttctg tctcggcgcg gtctccatcg
     5341 tcggatcgag aaagaccttc aaccacagcc ggctgttgtg cggcacgctg gtc
//
LOCUS       DATIMH010000781         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4228103, whole genome shotgun sequence.
ACCESSION   DATIMH010000781 DATIMH010000000
VERSION     DATIMH010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4228103"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VLI90_13670"
     CDS             complement(<1..244)
                     /locus_tag="VLI90_13670"
                     /inference="COORDINATES: protein motif:HMM:NF014577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HSV15303.1"
                     /translation="MCITLAAVTLPTTQTWRLIEMNMAKSLLFGTAASLIALTGAEAA
                     DLPVKAKAVEYVKVCSLYGAGFWYIPGTDTCIRIGGA"
     gene            complement(653..973)
                     /locus_tag="VLI90_13675"
     CDS             complement(653..973)
                     /locus_tag="VLI90_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV15304.1"
                     /translation="MLVDARQIKAARSFLRWRQDELARQTGLALSTIRRLERSEGPIE
                     ANFDTVQKIRQALESAGIEFIGSPNLGVRYWIRRETDAGPSEHDQGRAPMSGTGEFSR
                     PLAG"
     gene            complement(1382..>1645)
                     /locus_tag="VLI90_13680"
     CDS             complement(1382..>1645)
                     /locus_tag="VLI90_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15305.1"
                     /translation="VEIRQGDALQTLSVDLPETVDLLLLDGAKALYPEILDLVESRLR
                     PGAYIVADNADHSPEYLARVRAPTGGYLSTPFGGDVELSMRIH"
BASE COUNT          315 a          533 c          482 g          315 t
ORIGIN      
        1 ccgcgccgcc gatgcggatg caggtgtcgg tgcccgggat gtaccagaag cccgcgccgt
       61 agagcgagca gaccttcacg tactcgaccg ccttcgcctt gacgggaaga tcggcggcct
      121 cggcgccggt cagggcgatg aggctcgcgg cggtcccgaa caaaaggctc ttagccatgt
      181 tcatctcaat caatctccag gtttgtgttg tcgggagcgt gacggccgca agcgtgatgc
      241 acatccggtc ggtctcgtcg cccgctacat ccgtccgtca gctcacgcgt ctcgcgcatt
      301 ggatccgagg gaacgaaacg acttgagaag ctcccccgcc gcgcagcgac aggacgacaa
      361 atccggattc ttctcaatcc aaatattagt tcgtatatga agtaactcgt gaatgtagca
      421 ccgcaaacac agctggttca ctctcgatat ttaatgatca gataaagtat ctaatatccc
      481 agatgatagc tcttttgcac ttcgcgcttt cgcctaacga gatgcgcatc gattgcatcg
      541 cgacgaggat tcttctcgcg cgacatcttc atcggtgctg actctgaaaa acgatcttac
      601 ggctcgccgg cgcgaagcgg cattgtcgcg ttcgaacgat cctcaccgac aattatccgg
      661 cgaggggcct ggagaattcg ccggtgccgg acatcggcgc gcggccctgg tcgtgctcgg
      721 acgggccggc gtcggtttcg cgccggatcc agtagcgcac gcccagattc ggcgacccga
      781 tgaactcgat tcccgcgctt tcgagcgcct gccgaatctt ctgaacggtg tcgaagttgg
      841 cctcgatcgg cccttccgag cgttccagcc tgcggatcgt cgacagcgcc agcccggtct
      901 gccgggcaag ctcatcctgc cgccatcgca ggaaagagcg tgctgccttg atctgtcgcg
      961 catcaaccag catcgtcgct cccggctttc gaccggccgc acgcaacaga ttgcccggag
     1021 catgccaacc ggcagtccat cttcgaaccg cgctcaccac cgctcagtcc agcacgcatc
     1081 ttcacagcca aatgatcgct tcacccgtga agtaactcgc cgaatggcgc agcgcaaata
     1141 ttcgcgggtc acagtaattt gacctcggcg gcgaagcggc tttcccggcg ccgcggatgc
     1201 accctgtcat caaatcgtta cgacgaaacc gcagcccaga cgtctttcaa acagcttgcc
     1261 catgcaccgg ggcggcgttg gcgccgaccc atgcgcggcc gcgatgctgg caatgcgttt
     1321 gcgcgacgtt gcgtggcgcc gaaccataca ggatttcggc tgcggccgga tgagcgcgat
     1381 ttcagtggat ccgcatcgat agctcgacgt caccgccgaa cggcgtcgac aggtagccgc
     1441 cggtgggcgc gcggacccgc gccagatact cggggctatg gtcggcgttg tcggcaacga
     1501 tgtaggcgcc cggccgcagc cggctctcca ccagatccag gatctcggga tagagcgcct
     1561 tggcgccgtc gagcagcaac aggtcgacgg tttcgggcag atcgacgctc aatgtctgca
     1621 gtgcatcgcc ctgccggatc tcgac
//
LOCUS       DATIMH010000782         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3362037, whole genome shotgun sequence.
ACCESSION   DATIMH010000782 DATIMH010000000
VERSION     DATIMH010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3362037"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1354
                     /locus_tag="VLI90_13685"
     CDS             155..1354
                     /locus_tag="VLI90_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSV15306.1"
                     /translation="MALDAFIFDVDGTLLDSNALHVNAFVRTFAAHGYKVAPDRIAVE
                     IGKGGDNLIPAVLGESAEKKDGESMRQQQPKEFAKLAAEHPIRVFPKVRELLAEVRRR
                     RLKIVIATSSGKQQLETLSKYSGLDIAGEADVVVNADDTDNSKPAPDLIVAAVRKAKL
                     SPAQCVMIGDTPWDAESAKHGGVVTFGVLCGGANDTETLIGAGARHVYADPADLLAHL
                     DEAMTIASPSSIHLTQSRLEELMRQALDAAREGMANGEAPIGALLARGDGSPIARGWN
                     RLNQTQNKTAHAEMVTFAEAAGKTASDARDLILVTTLEPCVMCTGAAMEAAVDTIVYG
                     LRAPADAGTARVAPPRSPESQMPRIVGDILASESQALFQQWYERNKDAGEQAAYVRQL
                     LELHHAL"
     gene            1580..>2366
                     /locus_tag="VLI90_13690"
     CDS             1580..>2366
                     /locus_tag="VLI90_13690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSV15307.1"
                     /translation="MKSPDGKTNPAWYDVVSIPDYPAPPAGNHACDVCVVGAGIAGLT
                     SAYLLAKAGKSVVVLDEGPIASGQSGRTSAHLASEIDDRFIEIERLHGEANAKLAYES
                     HAAAIDLIERIARDEQIDCDFRRLDAYLFPLPSDPPDLLGREWAAAKRAGFRVAELLE
                     QHSLRAWDPSPCIRFGDQGRFHPLKYLVGLTKALDRLGVKIYTGCRVKDVSGAEPKKG
                     TPPIATVDEGAAKFEASGGIVVATNTPAPINDWFGIYTKQAPYR"
BASE COUNT          442 a          738 c          777 g          409 t
ORIGIN      
        1 cctctacccg gactaaccgc ttcagcggtt agtgcatttc attcgtcatt cgtcatccgg
       61 atttcattcg gcgattccga tttcgtcatt cggattttcc gccgaagcga tttcaacgtt
      121 cccgccccac cccccgatgc tctatcacca attcatggcc ttggacgcct tcatcttcga
      181 cgtcgacggc acgctgctgg acagcaacgc gctgcacgtg aatgccttcg tccgaacctt
      241 cgccgcccac ggttacaagg tcgcgcccga tcgcatcgcc gtcgaaatcg gcaagggcgg
      301 cgacaacctc attcccgcag tgctgggtga atctgcggag aagaaagacg gcgaatcgat
      361 gcgccagcag cagccgaagg aattcgcaaa gctggcggcc gagcatccca ttcgcgtctt
      421 tccgaaagtg cgcgagctgc tggccgaggt ccgccgccgc aggctcaaga tcgtcatcgc
      481 cacgtcgagc gggaagcagc agctcgagac gttgtcgaaa tactctggct tggacatcgc
      541 cggcgaagcc gacgtggtcg tcaacgcgga cgacaccgac aacagcaagc ccgcgccgga
      601 cctgatcgtg gcggcggtgc gaaaggcgaa actgtcgccg gcgcagtgcg tcatgatcgg
      661 cgacacgccg tgggatgccg agtcggccaa gcatggcggg gtcgtgacgt tcggcgtttt
      721 atgtggcggc gcgaacgaca ccgaaacgct gatcggcgcc ggcgctcgcc acgtgtacgc
      781 cgaccccgcc gatctgttgg cccatctgga cgaggcgatg acgatcgcgt cgccgagttc
      841 aatccatctc actcaatcgc gcctggagga actgatgcgc caggcgctgg acgcggcgcg
      901 cgaaggcatg gccaacggtg aagcgccgat cggcgcactg ctggcgcgcg gcgatggctc
      961 gccgatcgcg cgcggctgga atcgcctcaa ccagacgcag aacaaaaccg cccacgcgga
     1021 gatggtgacc ttcgccgagg ctgccgggaa gacggcgagc gatgcgcgcg atctgatcct
     1081 ggtcacgacg ctggagccgt gcgtgatgtg caccggcgcc gcgatggaag cggcggtgga
     1141 tacgatcgta tacggcctgc gcgccccggc tgatgcgggc acggcgcgcg tcgccccgcc
     1201 gcggagcccg gaaagtcaga tgccgcggat cgtgggggac atcctggcga gcgaaagcca
     1261 ggcgctgttt cagcagtggt acgagaggaa taaagatgca ggggaacagg cggcgtatgt
     1321 gcggcagttg ttggagctgc atcacgctct gtagcgatcg gcgcgtcgca cgtctcgctc
     1381 aactcggatc tctcgtagcc gcgaggcttt agcccgcgga ctgttcgagc aggaatgcgg
     1441 ttctgtgaaa tcgaacggtc cgcgggctaa agccgcgcgg ctgcgatgag ggtgcgagga
     1501 cattgcgcga taatggtttt ccatcgtccg gttttgcgcg ctcgcgcggt ccgcgaagcg
     1561 cgcgcatcat ccaagtgcca tgaaatcccc cgacggtaaa acgaatccgg cctggtacga
     1621 cgtcgtctcc atccccgatt atcccgctcc gcccgcgggg aaccatgcgt gcgacgtctg
     1681 cgtcgtcggg gctggtatcg cggggttgac cagcgcttac ttgctggcga aggcgggcaa
     1741 atccgtcgtc gtgctcgatg aaggtccgat tgcatccgga cagtcgggac gcaccagtgc
     1801 gcacctggcc agcgaaatcg acgaccgctt catcgagatc gaacgcctgc acggcgaggc
     1861 gaacgcgaag ctggcgtacg aaagtcacgc ggcggcgatc gacctgattg aacggatcgc
     1921 gcgcgatgag cagatcgact gcgatttccg ccggctggat gcgtacctgt tcccgcttcc
     1981 cagcgatccg ccggacctgc tcggccgcga atgggcggcg gcgaagcgtg ctggatttcg
     2041 cgtcgctgaa ttgctcgagc agcattcgct gcgggcgtgg gatccctcgc cgtgcattcg
     2101 cttcggcgac cagggacgat tccatccgct gaaatatctg gtggggttga caaaggcgct
     2161 cgatcggctg ggcgtgaaga tttacaccgg ttgccgtgtg aaggatgtgt cgggcgcgga
     2221 gccgaaaaag ggcacgccgc cgatcgcgac ggtcgatgaa ggcgccgcga agttcgaagc
     2281 atccggcggc atcgtcgtcg cgaccaacac gcccgcgccg atcaacgact ggttcggcat
     2341 ctacaccaag caggccccat atcgca
//
LOCUS       DATIMH010000783         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5389333, whole genome shotgun sequence.
ACCESSION   DATIMH010000783 DATIMH010000000
VERSION     DATIMH010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5389333"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VLI90_13695"
     CDS             complement(<1..569)
                     /locus_tag="VLI90_13695"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HSV15308.1"
                     /translation="MVGFCSSPQFIEHDTGPHHPERPDRIRAIYRALHDTRLLRSADP
                     FPDFRPDFGLHPIDVPPLVELSPTPADERWLLSVHPQQHVDRVKHLCAHGAVMDQGDT
                     PTEPRSFETARLAVGAVLSACDAVMNGTVKRAFAAVRPPGHHAEPLRAMGFCLFSNVA
                     IAARYLQQAHGLQHVAVVDFDVHHGNGTQA"
     gene            complement(588..1757)
                     /gene="galK"
                     /locus_tag="VLI90_13700"
     CDS             complement(588..1757)
                     /gene="galK"
                     /locus_tag="VLI90_13700"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HSV15309.1"
                     /translation="MAGAFDAVRQQFTEIFGGDGHIHVVRAPGRVNLIGEHTDYNDGF
                     VFPMAIEPHVLIICRARTDGKVRLASTLFPKQIVVFSVEQKIARGEPKWANYSKGIAA
                     ELIGAGIPLIGMDALVSNTLPMGGGLSSSAALEVGTGRAMLYLAGTTMEAQRLALLSQ
                     KAEHEYALVPCGIMDQTIVAAGKAGHALLLDCRDLSKQYIPIDPNELRVVIVNSMVKH
                     ELSGGEYAERRRQCEEGVAYFRKSNPDIRALRDVTMAQVIAAKNDLPDVVFRRCRHVV
                     GENVRTTEAAQQLTARKYERVGELMVQSHNSLRDDYEVSVEELDYLVAQSMTIKGVYG
                     ARMTGGGFGGCIVALVQPRSVDGLTSHLNKVYPTKFNKQPGIFVTTATEGAAVVE"
     gene            1932..>2363
                     /gene="tmk"
                     /locus_tag="VLI90_13705"
     CDS             1932..>2363
                     /gene="tmk"
                     /locus_tag="VLI90_13705"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSV15310.1"
                     /translation="MPNLHGKFIVFDGGEGCGKSTQAHLLRDTLEARGSHVLLVRDPG
                     TTEIGEQIREILLNPANDQMSMRCEMLLYMAARAQLMKETIVPALARGDVVVSDRFVS
                     STLAYQLGGDGLTTHDILRVAEIAVNHRWPDLTIILDMPIEG"
BASE COUNT          419 a          773 c          745 g          426 t
ORIGIN      
        1 gcctgcgtgc cgttgccgtg atgaacgtcg aagtcgacga cggcgacgtg ctgcaggccg
       61 tgcgcctgct gcagatatcg cgcggcgatg gcgacgttcg agaacaaaca aaaccccatc
      121 gcccgcagcg gttcggcgtg atgccccggc gggcgaaccg cggcgaaggc gcgcttgacg
      181 gttccgttca tcaccgcatc gcacgcgctc agcacagcgc cgacggccaa tcgcgccgtc
      241 tcaaaactcc gcggctccgt cggcgtgtcg ccctggtcca tcaccgcgcc gtgcgcgcac
      301 aggtgcttca cgcgatcgac gtgctgctgc ggatgcacgc ttaacagcca gcgctcatcc
      361 gcgggcgttg gcgacaattc caccagcggc gggacatcga tggggtgaag gccgaagtcg
      421 ggacgaaagt ccgggaaggg atcggcggat cgcagcaatc gcgtatcgtg aagcgcccgg
      481 tagattgccc ggattcggtc gggccgctcg gggtggtggg gaccggtgtc gtgctcgatg
      541 aactgcgggc tggagcagaa gccgaccatg agcggcgcct tgttctgtca ttcgacgacg
      601 gcagcgcctt cggtggcggt cgtcacgaag atgcccggct gcttgttgaa cttcgtcgga
      661 taaactttgt tgaggtgact cgtcagccca tcgacgctgc gcggctgcac cagcgcgacg
      721 atgcacccgc cgaacccgcc gccggtcatg cgggcgccgt atacgccttt gatggtcatc
      781 gactgcgcga ccaggtaatc cagttcctcg acgctcacct cgtaatcgtc gcgcagcgaa
      841 ttgtggctct gcaccatcag ttcgccgacg cgttcgtatt tgcgcgcggt cagttgctgg
      901 gcggcctcgg tcgtccgcac gttttcaccg acgacatgcc ggcatcggcg gaagaccaca
      961 tcgggcaaat cgttcttcgc cgcgatgacc tgcgccatcg tcacatcgcg cagcgcgcga
     1021 atgtcggggt tggacttgcg gaaataagcg acgccttcct cgcactgccg gcggcgctcg
     1081 gcatattcgc cgccgctcag ctcgtgcttc accatgctgt tgacgatgac gacgcgcagc
     1141 tcgttgggat cgatcggaat gtactgcttc gacagatcgc ggcaatccag cagcagcgcg
     1201 tgccccgcct tccccgcggc gacgatcgtc tgatccatga tgccgcaggg caccagcgcg
     1261 tattcgtgct cggccttctg gctgagcagc gccaaccgct gcgcctccat cgtcgtgccg
     1321 gccaaataaa gcatcgcccg gcccgtgccc acttccaacg cggcgctgct cgacaacccg
     1381 ccgcccatcg gcagcgtgtt ggagaccagc gcatccatgc cgatcagcgg aatgccggcg
     1441 ccgatcagtt cagccgcgat gccctttgaa tagttcgccc acttcggctc gccgcgcgcg
     1501 atcttctgct cgaccgaaaa caccacaatc tgctttggaa atagcgtgga tgccaatcgt
     1561 actttgccgt cggtgcgcgc ccggcagatg atcagcacgt gcggctcaat ggccatggga
     1621 aagacgaagc cgtcgttgta gtcggtgtgt tcaccgatca ggttgacgcg accgggggcg
     1681 cggacgacgt ggatgtggcc atcgccgccg aatatctcgg tgaactgctg acgaaccgcg
     1741 tcgaatgcac cagccatggc ggccgaatgt aggcgggggt atggcgcgcg gtcaagattg
     1801 atcttgttca ccagccgctt gcggcttagc gtcttttcat tttgggaaat gacgctaagc
     1861 cgcaagcggc tgggtaccgc aagccgctgt acagcatcgc ccccgcgtgt accatgcgac
     1921 gcatcgacaa catgcccaac ctccatggca agttcatcgt cttcgacggc ggcgaaggct
     1981 gcgggaaaag cacgcaggcg cacctgcttc gcgacacgct ggaagcgcgc ggctcgcacg
     2041 tgctgctcgt gcgcgatccc ggcaccaccg aaatcggcga acagatccgc gagatcctgc
     2101 tcaaccccgc caacgaccag atgtcgatgc gctgcgagat gctcctgtac atggccgctc
     2161 gggcgcagct catgaaagag acgatcgtcc cggcgctggc gcgcggcgat gtcgtcgtca
     2221 gcgaccgctt cgtctccagc acgctggcgt accaactggg tggcgacggc ttgacgacgc
     2281 acgacatcct gcgcgtcgcg gagatcgcgg tgaatcaccg ctggccggat ttgacgatca
     2341 tcctcgacat gccgatcgaa ggc
//
LOCUS       DATIMH010000784         1578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4229839, whole genome shotgun sequence.
ACCESSION   DATIMH010000784 DATIMH010000000
VERSION     DATIMH010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4229839"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VLI90_13710"
     CDS             complement(<1..386)
                     /locus_tag="VLI90_13710"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HSV15311.1"
                     /translation="MNRRLYFAALPVLGFTVWALAQTPGDPLIEGFRNVEVASVADAM
                     EQLYGTKAYMSHDMRPIAKTKFAGPAVTVLLKKEEHKDGSKASQGMLDAIDAAPPGSV
                     YVMVLEDGLDYAGIGALMSTAMKARGL"
     gene            complement(415..>1578)
                     /locus_tag="VLI90_13715"
     CDS             complement(415..>1578)
                     /locus_tag="VLI90_13715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV15312.1"
                     /translation="SRDLGLSKLQMSFVFSAFAVAYAGFEVFSAWWGQKIGARRVLAR
                     IVLWWSAFTMATAGAWNYSSMLVIRFLFGAGEAGAWPNAAMALSRWIPLRERGRVQGF
                     FFAAAHASGGITPLLVAWLLTLLPWRGVFVVCGCVGVIWAAAWYRWFRDEPREHPQVN
                     EAEAVLIESGRQLGGHAHHGAALWRVLASSPNVWCLCLSYLSNSYGSYFVMTWLPTYL
                     AESRGFEKQSLSLFAGLPLLLSVFGDISGGAVTDWAIRAFGLRLGRSVVCVAGYAVAA
                     IAMVAAVYANTPVMAATLIAVAVAASMFTLSPSWAACMDIGGENTAVLSAAMNTTGQI
                     GSILSPVITGWIVSTSADWQLPLLIMAGLYGFSAVLWMAVDPRKRLQARAAAA"
BASE COUNT          315 a          578 c          445 g          240 t
ORIGIN      
        1 aggccgcgcg ccttcatcgc cgtcgacatc agcgcgccga ttcccgcata gtccagcccg
       61 tcctcgagga ccatcacgta cactgagccg ggaggcgccg cgtcgatcgc gtccagcatt
      121 ccttgcgagg ccttggagcc gtccttgtgc tcttccttct tgagcagcac cgtcaccgcg
      181 ggcccggcga acttcgtctt cgcgatcggg cgcatgtcgt gcgacatgta cgccttggtt
      241 ccatacaact gctccatagc atcggcgacg gatgctacct ccacgttacg gaagccctcg
      301 atcagcgggt cgccaggcgt ttgcgccaac gcccatactg tgaagcccaa caccggcagc
      361 gcggcgaagt aaagccttcg gttcatgatt ctgtcccttt cacgcgctac tgtatcacgc
      421 agcggcggcc ctggcctgca accgtttgcg cgggtcgacc gccatccaaa gcacggcgga
      481 aaatccataa agtccggcca tgatcagtaa cggaagctgc cagtcggccg aggtcgaaac
      541 gatccagcct gtgatcaccg ggctgaggat cgacccgatc tgccccgtgg tattcatggc
      601 cgcgctcagc accgccgtat tctctccgcc gatatccatg caggccgccc aactcggact
      661 cagcgtgaac atcgacgcgg ccaccgccac cgcgatcagc gtggccgcca tcacaggcgt
      721 attcgcgtag accgccgcca ccatggcgat ggccgcgacc gcgtaccccg ccacgcaaac
      781 cacactgcga cccagtcgca gtccgaaggc gcgtatcgcc cagtccgtca cggcgccgcc
      841 gctgatatcg ccgaagacgc tgagcagcag cggcagtccg gcgaacaggc tgagcgactg
      901 cttctcgaac ccgcggctct ccgcgagata cgtgggcagc cacgtcatca cgaaatacga
      961 gccataactg ttcgaaagat aggacaggca aagacaccag acattcggcg acgatgcgag
     1021 cacgcgccac aatgccgcgc cgtgatgcgc atgtccgccc aactgccgcc cgctttcgat
     1081 cagcactgct tcggcctcgt tcacctgggg atgctcgcgc ggctcgtcac ggaaccagcg
     1141 gtaccaggcc gccgcccaga tcacgccgac gcagccgcac accacgaaca ctccacgcca
     1201 cggtagcaag gtcagcagcc acgcaaccag cagcggcgtg atcccgcccg acgcgtgcgc
     1261 cgccgcgaag aagaagccct gcacgcgccc tcgctcgcgc aacggaatcc agcgcgaaag
     1321 cgccatcgcg gcgttgggcc acgcgcccgc ttcgcccgcg ccaaacagga agcgaatcac
     1381 cagcatgctg gaatagttcc acgcgccggc cgttgccatc gtgaatgcgg accaccacag
     1441 cacgattcgc gccagcactc gtcgagcgcc gatcttttgt ccccaccacg cgctgaagac
     1501 ttcgaagccc gcgtaggcca ccgcgaaggc gctgaacacg aagctcatct gcagcttcga
     1561 aagcccgagg tcgcgcga
//
LOCUS       DATIMH010000785         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_768884, whole genome shotgun sequence.
ACCESSION   DATIMH010000785 DATIMH010000000
VERSION     DATIMH010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768884"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VLI90_13720"
     CDS             complement(<1..1137)
                     /locus_tag="VLI90_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15313.1"
                     /translation="MSAPTVDVPLAAPPKTARRSTRLLDAAVLIPVLIAGAILRVFLA
                     KTGHNYDMDCWQVAVDLMQHGRSVYANTARYNYGPVWMLVLHGLRFLQDHTAQSLHIT
                     GPEQFHVYVAGFLTLVDIAIALILAARFGTIAAIMFFLCPVSVLITGFHSQVDNFAVL
                     FGLIGWCILRPPSVQTPSWTRTIIAGVVFGISLATKHLLIFFPLWFLIGGRRALPPKR
                     ALVLAILTYGVFAASFVPFALAPADRAGIVEHVLRYRSQVDTGSMFIRAIDMIVPYGI
                     FERTQLLFHGAPNGLVLLFLSMMLVTGRLVAVNRPAEMFWFYLIALTAFSIAWADQYL
                     TIPLVACVVYRRHWASWMYILVSTAFLITSPRNLAAVGGRDFLYN"
     gene            complement(1274..>2926)
                     /locus_tag="VLI90_13725"
     CDS             complement(1274..>2926)
                     /locus_tag="VLI90_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSV15314.1"
                     /translation="VGGQARGEVASHLACETALREFTTAAPGTAPNQVLWKMFNAANL
                     AVYDDGLKNRAAGRMATTLLVGIFRNNELTVGHVGDCRLYLFQEGRLRRVTNDHSYAG
                     VQLKLGLITVEEAKNSPNRSLLTRSVGQDPMIRIDYHRIILNRGDRVMMCSDGLYSCV
                     SEGEISNIVTKQEPEAACRELIALAERRMGEDNISCQIAHVDAVEKLSYYGGKPIYER
                     IADDTMGTDLEAGQTLDGRFQLTDLISRSGMASIFKANDIQTGQVVALKVPFMQFESD
                     PGFYSRFEREEKIGTTLNHPYILRVEPIDTPKSRPYIVMEFLEGQTLAHLMRRVRPMP
                     EKDALTIASRICEALHYMHDHEVTHRDLKPENIMICNDGSIRIMDFGIAKFEGQRRLT
                     FGGFTPTMGTPDYMAPEQVKGRRGDPRTDIYALGAILYEMVTGSPPFEGANPFLIMNA
                     RLTGDPVPPRQKNENLSPQVEEIIMHAMARNPADRYPDARVMQAELAHPEKVNVTGRA
                     ARAVAPSPMKSTWARTRWYIAAAALPIAVALFFFLKAHLQWK"
BASE COUNT          530 a          855 c          995 g          546 t
ORIGIN      
        1 gttgtagaga aaatcgcggc cgccgacggc ggcgaggttg cgcggcgagg tgatcaggaa
       61 cgccgtgctc accagaatgt acatccacga tgcccagtgc cggcgataga cgacgcacgc
      121 gaccagcggg atggtcagat attggtccgc ccaggcgatg gagaacgcgg tgagcgcgat
      181 gagatagaac cagaacattt cagcggggcg attgacggcg acgagccggc cggtgacgag
      241 catcatcgag aggaatagca gcaccagacc gttgggcgcg ccgtggaaca gcagctgcgt
      301 gcgttcgaag atgccatatg gcacgatcat gtcgatcgcg cggatgaaca tcgagccggt
      361 gtcaacctgc gatcgatagc gcagcacgtg ctcgacgatg ccggcgcggt cggcgggcgc
      421 cagggcaaac gggacgaatg acgcggcgaa gacgccgtag gtcaggatcg ctaagacgag
      481 ggcgcgtttc ggcggtagcg cgcggcggcc gccgatcagg aaccacagcg ggaagaatat
      541 cagcaggtgt ttggtggcga gcgagatgcc gaagacaacg ccggcgatga tcgtgcgcgt
      601 ccagcttggc gtctgcaccg atggcgggcg cagaatacac catccgatga gcccgaagag
      661 caccgcgaag ttgtccacct gcgaatggaa gccggtgatc agcactgaca cggggcaaag
      721 gaagaacatg atcgcggcga tcgtgccaaa gcgggcggcg aggatgagcg cgatcgcgat
      781 gtcaaccaac gtgaggaacc cggcgacgta gacgtggaac tgctctgggc cggtgatgtg
      841 cagcgactga gcagtgtgat cctggaggaa gcgcagcccg tgcaggacga gcatccagac
      901 gggaccgtag ttgtatcggg cggtattcgc gtacacgctg cggccgtgct gcatcagatc
      961 gaccgcgacc tgccaacagt ccatgtcgta attgtggccg gttttcgcga ggaagacgcg
     1021 gaggattgcg ccggcgatca aaaccgggat cagcacggcg gcgtcgagca gacgggttga
     1081 gcggcgcgct gtctttggtg gcgcggccag gggaacgtca acggtggggg cgctcatgcg
     1141 aggcagctta gcggttacgg tatcgtcagt gaaattttga tccgccgctt gcggcttagc
     1201 gtcttttcct tgcgtcgttt ccttgcgtcg tttccttgcg ccctgaaaca gatgctaagc
     1261 cgcaagcggc aggtcatttc cactgcagat gcgcctttaa gaagaagaac agcgccaccg
     1321 cgatcggcag cgcggcggcg gcgatgtacc agcgggtgcg ggcccaggtg ctcttcatcg
     1381 ggctcggggc gacggcgcga gcggcgcggc cggtgacgtt taccttttcc gggtgcgcta
     1441 gttccgcctg catcacgcgc gcatctgggt aacggtccgc tgggttgcgc gccatcgcgt
     1501 gcatgatgat ctcttcgacc tgcgggctga ggttctcgtt cttctgccgc ggggggaccg
     1561 ggtcgccggt caggcgcgcg ttcatgatca ggaatgggtt ggcgccttcg aacggcggcg
     1621 agccggtcac catctcgtat agaatcgcgc ccagcgcata gatgtccgtg cgcgggtcgc
     1681 cgcgacggcc cttcacttgc tcgggggcca tgtagtcggg cgtgcccatc gtcggggtga
     1741 atccgccgaa ggtcaggcgg cgctggcctt cgaatttggc gatgccgaag tccatgatgc
     1801 ggatcgaccc gtcgttgcag atcatgatgt tctcgggctt caggtcgcgg tgcgtcacct
     1861 cgtggtcgtg catgtaatgc agcgcctcgc agattcggct ggcgatcgtc agcgcatcct
     1921 tctccggcat cgggcgcacg cggcgcatca ggtgcgccag cgtctggcct tccaggaact
     1981 ccatcacgat gtatggccgg ctcttgggcg tgtcgatggg ttcgacgcgg aggatgtatg
     2041 ggtgattcag cgtcgtgccg atcttttcct cgcgctcgaa gcgcgagtag aaaccggggt
     2101 ccgactcgaa ctgcatgaac ggcaccttga gcgcgacgac ctggccggtc tgaatgtcgt
     2161 tggccttgaa gatcgacgcc attccgctgc ggctgatcag gtccgtcagc tggaatcgac
     2221 cgtcaagcgt ttggccggct tctaagtctg tgcccatggt gtcgtccgcg atgcgttcgt
     2281 aaatcggctt gccgccgtag tagctcagct tctcgaccgc atcgacatgc gcgatttggc
     2341 aggagatgtt gtcctcgccc atccgccttt ccgccaaggc gatcaactcc cggcaggcgg
     2401 cctcgggttc ctgcttggtg acgatgttgg agatctcgcc ttcgctgacg caggagtaca
     2461 aaccatcgct gcacatcatc acgcggtcgc cgcggttgag gatgatgcgg tgatagtcga
     2521 tgcggatcat cggatcctgc ccgaccgatc gcgtgagcag cgagcggttc gggctgttct
     2581 tcgcttcctc aaccgtgatg agcccgagct tcaactgaac gccggcgtag gaatgatcgt
     2641 tggtgacccg ccgaagcctg ccttcctgga acaggtacaa ccggcaatcg ccgacatgac
     2701 cgacggtcaa ctcgttgttg cggaagatgc cgaccagcaa tgtcgtcgcc atccgccccg
     2761 cggcgcggtt cttcagaccg tcatcgtaaa ccgccaggtt cgccgcgttg aacatcttcc
     2821 acagcacctg gttgggcgcg gtgcccgggg cggcggtggt gaactcgcgc agcgccgtct
     2881 cgcaagccag atggcttgcg acctcgccgc gcgcctgccc accgac
//
LOCUS       DATIMH010000786         1773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3364494, whole genome shotgun sequence.
ACCESSION   DATIMH010000786 DATIMH010000000
VERSION     DATIMH010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3364494"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VLI90_13730"
     CDS             complement(<1..587)
                     /locus_tag="VLI90_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15315.1"
                     /translation="MKVRWIFTVFACVMSAQTLELPKTIRQGGTLRIHGPGSAVTARM
                     NDRTIRLFRQADGGSFGLMPVSVDQKPGAYKVGLLDQGGATVAAASVEVVDAHFRKQN
                     VTIAESIAELKPSPGESETVAAFRNAVSDALYWSEPLELPVRGCMTSPFGVQRYLNGK
                     ATGSAHSGLDQRSPAGTPIHAVDGGVVKIVREWNL"
     gene            704..1018
                     /locus_tag="VLI90_13735"
                     /pseudo
     CDS             704..1018
                     /locus_tag="VLI90_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
     gene            complement(1015..>1773)
                     /locus_tag="VLI90_13740"
     CDS             complement(1015..>1773)
                     /locus_tag="VLI90_13740"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HSV15316.1"
                     /translation="EPGDAIAVTFTRMRMNRNFGFTAYRLGVYAINPEQVEGLYPNTR
                     PRGAVLPDRENILRWDLDLAHNTVKLRDPVSKVHQMQFAAKPMLGCVGVAAPGVFGPT
                     SGISGNYGGNMDYNRINEGATVVLPVFHSGALLFIGDGHALQADGEPTGTGIETSLDV
                     TFTVELRKRAGVNNPRVETPESIIAVGSQPEFVSSLDRSLRVATSDMVDWLVRDYKME
                     PWAAHMLIGYQGQYDVVTVAGSMALRIPRSALPH"
BASE COUNT          336 a          579 c          547 g          311 t
ORIGIN      
        1 tgcaggttcc attcccgcac gatcttgacg acgccgccat ccaccgcatg gatcggggtc
       61 cctgcgggcg accgctgatc gaggccgctg tgagcgcttc cggtcgcctt gccgttcagg
      121 taccgttgca cgccgaaggg cgacgtcatg cagccgcgca ccggcaactc cagcggctca
      181 gaccagtaga gcgcatccga tacggcgttc cgaaaagccg caacggtctc ggactctcca
      241 ggcgagggct tcaactcggc aatgctctcg gctatggtga cgttctgttt ccggaaatgc
      301 gcgtcgacaa cctccaccga ggctgccgcc acggtcgcgc cgccctggtc aagtaacccg
      361 accttatatg cgccgggctt ctgatcgacg ctcaccggca tcaggccgaa cgagccgccg
      421 tccgcctgcc gaaacaaccg gatggtgcgg tcgttcatcc gcgcggtgac cgcggaaccc
      481 ggtccgtgga tccgcagcgt gccgccctgc cggattgttt tcggaagttc cagcgtctgg
      541 gcagacatga cgcacgcgaa gacggtgaaa atccaacgca ccttcatagg atagcgggca
      601 acgccagtaa gcgaacaaaa gcccgcggcc ggtgtccatt gctgcatgga ttggcgcgta
      661 ctgagagcgc agcacgcggc cgaaacgtgc ggcggccgtc acacatcggc agatggcgcg
      721 cggtctgggc ggtctcgcca ccgtggcggc gacggcgccg ttcgtcggga tcatcgggac
      781 actgctcggg atcttcgatg cgttccgcgg ttgtggctgc gaaaaaaccg cttacatggc
      841 ggcagtcgcc ggctatttat ccgaatcgct gatgccgtct gaagccggac tcgcggtcgc
      901 aatcatggcc tcgaccgccc accagtacct gagcgcccgg ctggattact tcgacatgga
      961 gatgcggaac gccgcgagag aaatgccggg ctatctcgcg ccgctcacac gaccttaatg
     1021 cggcaaggcg cttcgcggga tccgcaacgc catcgagccg gcaacggtca ccacgtcgta
     1081 ctgaccctga tacccgatca gcatgtgcgc ggcccacggc tccatcttgt aatcgcgcac
     1141 gagccagtcc accatgtccg acgtcgccac gcggagggaa cggtcgagcg aactgacgaa
     1201 ttcgggctga ctcccgacgg cgatgatcga ctcgggcgtc tcgacacggg gattattgac
     1261 accggcccgc ttccgcaact caaccgtgaa cgtaacgtcg agagaggtct cgattcccgt
     1321 gccggtcggc tcgccgtcgg cttgtagcgc gtgaccgtca ccaatgaaga gcagggcgcc
     1381 cgagtggaaa accggcagga ccaccgtcgc gccttcgttg atgcggttgt aatccatgtt
     1441 gccgccataa tttcccgaga tgccgctggt cgggccaaat actcctggcg cggcaacgcc
     1501 aacgcatccc agcatcggtt tggcggcaaa ctgcatctgg tggaccttgc tgacggggtc
     1561 gcgcagtttc accgtgttgt gcgcgagatc cagatcccag cggaggatat tctcacgatc
     1621 cgggaggacg gcgccccggg gacgcgtatt cggatagaga ccctccacct gctcgggatt
     1681 gatcgcgtaa acaccgaggc gatacgccgt gaagccaaaa ttccgattca tccgcatgcg
     1741 agtgaatgtc acggcgatcg catcgcccgg ctc
//
LOCUS       DATIMH010000787         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3078391, whole genome shotgun sequence.
ACCESSION   DATIMH010000787 DATIMH010000000
VERSION     DATIMH010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3078391"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VLI90_13745"
     CDS             <1..938
                     /locus_tag="VLI90_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15317.1"
                     /translation="AFAITHLHRRRVLVGCALLLVAGSLVLSPWLLRTFAWSGGNPVF
                     PLAMKVLGKAHFTDGQVRRFIIAHSAPADERAPLARLRVTWNDVIAQWQFGFVILPLA
                     LAAAAARWRQRGTWALLFTAAIIFIVWIGFTHLLGRFLIMLVPIAALLIGRVRWGRTS
                     QVAAVVLLIVSAVFSWRQMYWRLAQTTRDPLRSALIGRQDLTFTIPEELLEMRDDNNK
                     QVGLVGDAGAFLYQIPMTRLHYRTVFDVPADDSDPIDAWVGPQAKGDRDWLLVVNPTE
                     INRLHRTYIGIPALPADWASRGDQPFVLRGDRVGK"
     gene            complement(980..2785)
                     /locus_tag="VLI90_13750"
     CDS             complement(980..2785)
                     /locus_tag="VLI90_13750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV15318.1"
                     /translation="MTETTQLRRWTGPALVALAFLAMLTWSWGTWPDPLVDFGAQLYF
                     PWRIHDGGAVLYRDLAFYNGPLSQYLNAFWFTIAGVSMRTIIFANLAICAAVLALIYR
                     LSRHASGEIAAAAAGVTFAVFFAFAQGVGVGNYNWVTPYTHEITHGVALGLLAIACLH
                     RFHRTGRTRWLTIGGAVLGLTFLTKAEPFIATLGAITAQLLSHWWLVRPARNQVARDL
                     LAFIAAAAAPPLIAFALLWMTMPLRDAACGLAGSWPWVFDRRIVALPFYRNISGADDV
                     GGNLRAMLDWSLAYAAVLVPAGVIALFVRRKAARWIVTSLVLVILGGILYAIFWDTNW
                     NLMLRPLPLCLLLALAVATLPLVRKSESSPRAPLRLALVVFSIALVAKIALNANVYHY
                     GFALAMPGTLVLVAAFAGDVPNWICRRGGSGSVLRAAGLAAWIAAAAAMLYLDNGYFT
                     SKRWTIGQDGDRFRGATRGLEVQRMTQLIGQLVPPNGTVAVFPQGLMLNYLTRRAASI
                     KHVNFMPPEVLAAGEPQILADLAAHPPDAVVVNASTIIDGEFSMDVTYRYGRDTLAWI
                     TQHYTPVATSELPPPYPTFYKLVLMKRKVNAGLRK"
     gene            complement(2909..>3380)
                     /locus_tag="VLI90_13755"
     CDS             complement(2909..>3380)
                     /locus_tag="VLI90_13755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV15319.1"
                     /translation="FIIVIQGGMIGRLTKMFGEWSVAIAGAVFVTIGMALYMWCGWLP
                     ALWLLLLAGTANATGRSLQQPPIATLISKFSDRSEQGAVFGLYHGLGSLARVIGPLSA
                     GLLYGPLHKTAPFLAAGVVTTAVAMWIAGLWWSLPATAPLVSETAMAQAAVETT"
BASE COUNT          610 a         1088 c         1143 g          539 t
ORIGIN      
        1 tcgcgttcgc catcacgcat cttcaccgtc gccgggtgct cgtgggctgc gcgttgttac
       61 tcgtcgctgg atcgcttgtc ctctcgccat ggctgctgcg gaccttcgcc tggtctggcg
      121 gcaatcccgt tttcccactg gcgatgaagg tgctgggcaa agcccatttc acggatgggc
      181 aggtgcggcg gttcatcatc gcgcacagcg ccccggctga tgagcgcgcc ccgctggcgc
      241 gactgcgcgt cacgtggaac gacgtcatcg cgcaatggca attcggcttc gtgatcctgc
      301 cactggcgct cgccgccgcc gccgcgcgct ggcggcaacg cggcacgtgg gcattgctgt
      361 tcaccgcggc aatcatcttc atcgtgtgga tcggattcac ccacctgctg ggccggttcc
      421 tgatcatgct cgtgccgatc gcggccctgc tcatcggccg cgtgcgctgg gggcgcacgt
      481 cgcaagtcgc cgcggtcgtg ctgctcattg tctccgccgt cttcagttgg cgccagatgt
      541 actggcgact ggcccagacg acgcgtgacc cgctgcgctc cgccctgatc ggccggcagg
      601 acctgacctt cacgattcca gaagagctgc tcgagatgcg cgacgacaac aacaagcagg
      661 tcggcctcgt cggcgacgcc ggcgcgttcc tctaccagat cccgatgacg aggctgcatt
      721 atcgcaccgt cttcgacgtg cccgccgacg acagcgatcc gatcgacgcg tgggtcggcc
      781 cgcaggccaa gggcgaccgc gactggctgc tcgtcgtgaa tcccacggag atcaaccggc
      841 tgcaccgcac gtacatcggc atcccggcgc tgccggccga ctgggcatcg cgcggcgatc
      901 aacccttcgt cctacgcggc gaccgggtgg ggaagtaacg cgagcaggca acgacgaagc
      961 actcatcagc tcgttcacgt tatttgcgta gacccgcgtt cacttttcgc ttcatcaaca
     1021 ccagcttgta gaacgtcgga tacggcggcg gcaattccga tgtcgcgacc ggcgtgtaat
     1081 gctgcgtgat ccacgccagc gtatcgcggc cgtagcgata cgtgacgtcc attgaaaatt
     1141 ccccgtcgat gatcgtgctg gcgttgacga ccactgcatc gggcggatgt gccgccaggt
     1201 ccgccaggat ctgcggctcg ccggcggcga gcacttcggg cggcatgaag ttcacgtgct
     1261 tgatcgacgc ggcgcggcgc gtgaggtagt tcagcatgag cccctgcggg aagaccgcga
     1321 cggtgccgtt gggcgggacg agttggccga tcagctgagt catgcgctgc acttcgagcc
     1381 cgcgggttgc gccgcggaat cggtcgccgt cttggccgat cgtccagcgc ttgctcgtga
     1441 agtaaccgtt gtcgagatag agcatcgccg ccgccgcggc gatccacgcg gccagacccg
     1501 ccgcgcggag aacactcccg ctgccgccgc ggcggcagat ccaattgggc acatcaccag
     1561 cgaaggcggc gacgaggacc agcgtgccgg gcatcgccag cgcgaagccg tagtgataga
     1621 cgttggcatt gagcgcgatc ttcgcgacga gcgcaatcga gaagacgacc agggccaggc
     1681 gcagcggcgc ccgcggtgat gattctgatt ttcgcactag cggcagcgtc gcaacagcga
     1741 gcgccagcag caggcaaagc ggcagcgggc gcagcatcag gttccagttg gtgtcccaga
     1801 agattgcata gaggatgccg ccgaggatca ccaggaccag cgacgtcacg atccatcgcg
     1861 ccgccttgcg gcggacgaac agcgcaatca ctcccgctgg aacaagcacg gcggcgtacg
     1921 cgagcgacca gtccagcatg gcgcgcaagt tgccgcccac atcgtcggcg ccggagatgt
     1981 tgcgataaaa cggaagcgcg acgatgcggc ggtcgaagac ccacggccac gaaccggcga
     2041 ggccgcaggc ggcgtcgcgc aggggcatcg tcatccagag caacgcgaac gcgatcagcg
     2101 gcggtgcggc agcggcggcg atgaatgcga gcaggtcgcg agcgacctga ttgcgcgcgg
     2161 gtcgaacgag ccaccaatgg ctgagcagct gtgccgtgat ggcgccgagc gtcgcgataa
     2221 acggttcggc tttcgtcagg aacgtcagac cgaggacagc gccgccgatg gtaagccagc
     2281 gcgttcgacc tgtacggtgg aaccggtgga ggcacgcgat tgcgagcagt cccagcgcca
     2341 cgccgtgcgt gatctcgtgc gtatagggcg tcacccagtt gtagttgccg acgccaacgc
     2401 cttgggcaaa ggcgaagaag acggcgaacg tcacccccgc ggcggcggcg gcgatctcgc
     2461 cggaggcgtg ccgcgacagg cggtagatca gcgcgagcac ggcggcgcag atcgcgaggt
     2521 tcgcgaagat gatcgtccgc atgctcacgc cggcgatcgt gaaccagaat gcgttgaggt
     2581 attgcgacag cggcccgttg tagaacgcga gatcgcggta gaggaccgcg ccaccgtcgt
     2641 ggatgcgcca ggggaagtag agctgcgcgc cgaagtccac cagcgggtcg ggccaggtgc
     2701 cccaggacca ggtgagcatg gcgagaaatg cgagcgcgac gagcgcgggg ccggtccatc
     2761 gtcggagttg ggtcgtttca gtcatgtgaa tcagccgctt gcggcttggc agacccggcg
     2821 gcgtgtattg cgaagccgca agcggcgggt gcgttcaagc ggcgagcgcg ttcacaagtg
     2881 cgtgcttggt tccgatggcg agcaaaagct acgttgtctc caccgccgcc tgcgccatgg
     2941 cggtttctga cacaagcggc gcggtcgccg gcagcgacca ccacagcccc gcgatccaca
     3001 tcgcgacggc cgtcgtcacc acgccggccg cgaggaacgg cgcggtcttg tgcagcgggc
     3061 cgtagagaag ccccgcgctc aaagggccaa tgacgcgcgc aaggcttcca agcccgtgat
     3121 acaaaccaaa gacggcgcct tgttcggatc gatcgctgaa cttgctgatc agcgtcgcga
     3181 tgggcggctg ctgtaaactg cgaccggtgg cgttggcggt gccggccagc agcagcagcc
     3241 acagtgccgg cagccagccg caccacatgt acagtgccat cccgatcgtg acgaagacgg
     3301 cgccggcgat ggcgacggac cattcgccga acattttagt caaccggcca atcatgccgc
     3361 cctggatcac gatgatgaag
//
LOCUS       DATIMH010000788         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1635757, whole genome shotgun sequence.
ACCESSION   DATIMH010000788 DATIMH010000000
VERSION     DATIMH010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635757"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VLI90_13760"
     CDS             complement(<1..186)
                     /locus_tag="VLI90_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15320.1"
                     /translation="MLLPPTVLDEALAGVASVGAANTGVVTGGGSVTGGGLLIGPGSP
                     TTTGGGSFNGGGSVTTGG"
     gene            197..1666
                     /locus_tag="VLI90_13765"
     CDS             197..1666
                     /locus_tag="VLI90_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HSV15321.1"
                     /translation="MLGASDAGESGLAYTWATKTAPSGATVTFSANSANAAKNTIATF
                     SKAGAYSFTVTITDASGQSVQSSVDVTVNQTLSSIAVSPGTASVDDGATQAFSAKGVD
                     QFGAAMKTQPSFAFTIDAGGKGSIDGNSGVFSAPAVGAGAGSATVRATSGSISGTALV
                     TINNVAVTPPPVTTPPPVTTPPPSDPGVTPPPVAGNFNETFGDEFNAAPSSGVWVNRI
                     WDMSTQAGELENYTSNNVTVSNGVLSLTAKKENSNGMNYTSGLISTGGIAGKTAAGFS
                     FKYGYVEARMKLTKGNGLWPALWMMPTPDNNGNYHDGDGEIDIMEQVGYAPNTTEAHL
                     HHNGTQGKSFNTGTDLSAGFHTYGIDWQADHLTWYFDGKPFYTITSNVPNVSEYLILN
                     LAIGDANSWPGAPDGNTQFPSSMQIDYVRVFQSPGQSAVGAGTLLGNASSASSNSSMA
                     MQSSGGVRFTPSNASSVVDAASGSTNADGSDLQSLAAAG"
BASE COUNT          354 a          596 c          567 g          262 t
ORIGIN      
        1 gccgccggtg gtgacggatc ctccgccgtt gaacgatccc ccgccggtcg tcgttggcga
       61 tccggggccg atcaacaatc ccccgccggt gactgaccca ccgccggtga cgacgccggt
      121 gttggcggcg ccgacgctgg cgacgccggc gagcgcttcg tccagcacgg tcggtggcaa
      181 gagcacgaac ttatcggtgc tcggcgcatc cgacgccggt gagagcggtc tggcttacac
      241 ctgggcgacc aagacggctc cgtctggcgc cacggtgacg ttcagcgcca acagcgcgaa
      301 cgctgcgaag aacacgatcg cgacgttctc caaggccggc gcgtattcgt tcacggtgac
      361 gatcaccgat gcatcgggcc agagcgtgca gtcatcggtt gacgtgacgg tcaaccagac
      421 gctcagcagc atcgccgtca gccccggcac cgcttctgtt gatgacggcg cgacgcaggc
      481 gttcagcgcg aaaggcgtcg atcagttcgg cgcggcgatg aagacgcaac cgagcttcgc
      541 gttcacgatc gacgccgggg gcaagggctc gatcgacggc aactccggcg tgttcagcgc
      601 gccggccgtc ggcgcgggcg cgggctcggc caccgtgcgc gcgaccagcg ggtcgatcag
      661 cggcaccgcg ttggtgacga tcaacaacgt cgccgtcaca ccgccgccgg tgacgactcc
      721 cccgccggtg acgacaccac caccgagcga tcccggcgtc accccgccgc cggtcgcggg
      781 caacttcaac gaaacgtttg gcgatgaatt caacgccgcc cccagcagcg gcgtgtgggt
      841 caatcgcatc tgggacatga gcacccaggc cggcgaattg gaaaactata cgtcgaacaa
      901 cgtcaccgtc tccaacggcg tgctgtcgct gacggcgaag aaggaaaact cgaacggcat
      961 gaattacacc agcggtttga tcagcaccgg cggcatcgcc ggcaagaccg ccgcgggctt
     1021 ctcgttcaag tatggttacg tcgaagcccg catgaagctg accaaaggca acggcctttg
     1081 gcccgcgctg tggatgatgc cgacgcccga caacaacggc aactatcacg acggcgatgg
     1141 tgagatcgac atcatggaac aagtcggcta cgcgccgaac accaccgaag ctcacctgca
     1201 ccacaacggc acgcagggca aaagcttcaa caccggcacc gatctgtccg cgggcttcca
     1261 cacctacggc atcgattggc aggccgatca cctgacgtgg tacttcgacg gcaagccgtt
     1321 ctacacgatc acgtccaacg tcccgaacgt gagcgagtac ctcattctga acctggcgat
     1381 cggcgacgcc aacagctggc ccggcgcgcc tgatggcaac acgcagttcc cgtcgtcgat
     1441 gcagatcgac tacgtgcgcg tgttccaatc ccccggccaa agcgcggtcg gcgccggcac
     1501 gctgctgggc aacgcgagca gcgcgtccag caacagcagc atggcgatgc aaagcagcgg
     1561 cggcgtgagg ttcacgccga gcaacgcgtc gagcgtggtc gatgccgcca gcggctcgac
     1621 caacgcggac ggcagcgacc tacagtcgct ggccgcggcg gggtgagcgt cgggcagcgt
     1681 ttgttgatgc aacttaaagg gacgccatcg cgccacgcga tggcgtcctc ttttccaaat
     1741 gatgagcggg agcagacgtt caagacggat cgtcgcgca
//
LOCUS       DATIMH010000789         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3078603, whole genome shotgun sequence.
ACCESSION   DATIMH010000789 DATIMH010000000
VERSION     DATIMH010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3078603"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VLI90_13770"
     CDS             <1..570
                     /locus_tag="VLI90_13770"
                     /inference="COORDINATES: protein motif:HMM:NF028352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HSV15322.1"
                     /translation="HVNATILPAGGADFFTAILQSRGFSKRTTASPPLDLRNHLMVCG
                     SASESSRSFVDAAAASWVCPMPDVVFAGTHGHEDIGAWRDAILELMHASRRAVIAIRQ
                     PAQPDRAEPLRKTIAEVVARILAVAQPPTLLIEGGATAAAVFERLEWRTLKVEQQLAP
                     GVVALRPMESPHQRLVLKPGSYPWPTGVA"
     gene            complement(543..2024)
                     /locus_tag="VLI90_13775"
     CDS             complement(543..2024)
                     /locus_tag="VLI90_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HSV15323.1"
                     /translation="MTYDITNFGANRDGETVTTAQMQAAIDACGGAGGGTVNVPSGRY
                     VTGSLWLRSNVTLHLEAGATLVGSPRFDDFPIWSSRWEGAGVKAGRAALICGEGLDNV
                     AITGRGTIDGRGAIWWEQQAKAPGKIRRPFLFRVVDCRNVLVEGVTFRNSPMWTVTPL
                     ACDNVVINRITIINPPDSPNTDGINPDSCRNVRISDCHVDVGDDCITIKSGKEDDGRQ
                     QLRPCENIAVTNCTLVHGHGGVVIGSEISGGVRNVAISNCVFVGTDRGIRIKARRGRG
                     GVVEDVRASDLIMDGVNCPIVVNLFYGCGAWNDQKVLDQGAHPVNDGTPRFRRLRYSN
                     ITARRIKFAAAYILGLPEMYVEDLIIENSSFFLDAQGTDAGPPAMAPGVPDLCRAGII
                     ASSVRQLTLRQLDITDQIGPAISISKAKEMRLLDLTLRSPSIDSPLVNLEEIEGAHIA
                     GCYAPQDASLPVQISGEHSSNIIVHDQEFDVDESAQATPVGQG"
     gene            complement(2209..>2480)
                     /locus_tag="VLI90_13780"
     CDS             complement(2209..>2480)
                     /locus_tag="VLI90_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15324.1"
                     /translation="AFANDPTIILADEPTGNLDLHTGRLIIELLRQLNQERGVTIISA
                     THDHKMLAVSDRILWISDGRVDRIQKREELKIAEGVIESTTAAPS"
BASE COUNT          407 a          806 c          791 g          476 t
ORIGIN      
        1 cacgttaacg ccacgatctt gcccgcaggc ggcgcggact tcttcacggc gatcctgcag
       61 tcgcgcggct tctcgaagcg aaccaccgca tcgccaccgc tcgatctgcg gaaccatctc
      121 atggtctgcg gcagcgccag cgaatccagc cgcagctttg tcgatgccgc cgcagcatcg
      181 tgggtatgcc cgatgccgga tgtggtgttc gcgggcacgc atggacacga agacatcggc
      241 gcgtggcgcg acgccattct cgagctaatg cacgccagtc gccgggcagt aatcgccatt
      301 cgacagcccg cgcaacctga tcgcgctgag ccgctgcgca agacaatcgc ggaagtcgtc
      361 gcgcgcatac tagcagtcgc tcaaccgccc acgctgctca tcgaaggcgg cgccaccgcg
      421 gcggccgttt tcgagcggct ggaatggcgc acgctaaagg ttgaacagca gctagcgccc
      481 ggcgtggtcg cgcttcggcc gatggaaagc ccccaccagc gactggtcct caagcccggc
      541 agttacccct ggccgaccgg cgtggcctga gcggattcgt ccacgtcgaa ttcctgatcg
      601 tgcacgatga tgttcgagct gtgctcccca gaaatctgca cgggcagcga cgcatcctgc
      661 ggcgcatagc agccggcgat gtgggcgcct tcgatttctt cgaggttcac cagcggactg
      721 tcgatcgacg gcgaccgcag cgtgagatcg agcagccgca tctccttcgc cttggaaatg
      781 ctgatcgcgg ggccgatctg gtcggtgatg tccaactgcc gcagcgtcag ctggcggacg
      841 ctactggcga tgatgcccgc gcggcacagg tccggcacgc ctggcgccat cgccggtggg
      901 ccggcgtcgg tgccttgggc gtccaggaag aacgagctgt tctcaatgat caaatcttcg
      961 acgtacatct ccggcagccc gaggatgtat gcggcggcga acttgatccg ccgagcggtg
     1021 atgttgctgt agcgcagccg gcgaaaccgc ggcgtgccgt cgttcactgg atgggcgccc
     1081 tggtccagca ccttctgatc gttccacgcg ccgcagccgt agaacaggtt cacgacgatc
     1141 gggcagttca caccgtccat gatcaagtcg ctggcgcgca cgtcttcgac cacacccccg
     1201 cggccgcggc gggctttgat gcggattccc cggtcggtgc cgacgaacac gcagttggag
     1261 atcgcgacgt tgcgcacgcc gccgctgatc tcgctgccga tgaccacgcc gccgtggccg
     1321 tgcacgagcg tgcagttggt gacggcgatg ttttcgcatg gccgcagctg ctgccggccg
     1381 tcgtcttctt tgccgctttt gattgtgatg caatcatcgc cgacatcgac gtggcaatcg
     1441 ctgatgcgca cgttgcggca cgaatcggga ttgatgccgt cggtgttggg cgagtccggc
     1501 ggattgatta tggtgatgcg attgatcacg acgttatcac acgccaacgg cgtgaccgtc
     1561 cacatcggtg aattgcggaa ggtcacgcct tcgaccagca cgtttcggca atcgacgacg
     1621 cggaacaaga acggccggcg aatcttgccg ggcgctttcg cctgctgctc ccaccagatc
     1681 gccccgcggc catcgatcgt tccgcgcccg gtgatggcga cgttgtccaa cccttcgccg
     1741 cagatcagcg ctgcgcgccc ggccttcacg ccggcgcctt cccagcggct ggaccagatg
     1801 gggaagtcgt cgaagcgcgg gctgccgacg agggtggcgc cggcttccag gtgcagcgtg
     1861 acgttgctgc gcagccagag cgagccagtc acatagcggc ctgaagggac gttcacggtg
     1921 ccaccaccgg cgccgccgca ggcgtcgatc gctgcctgca tctgtgcggt ggtgacggtt
     1981 tccccgtcac ggttcgcgcc gaagttcgtt atgtcgtacg tcatggagcc tctttctggt
     2041 cccctcgccc ggtacgccgg gagagggtta gggtgagggt gattttggga acgatgaacg
     2101 atgaacgatc agcattcgcc ctttagtcat cgttccgcgt tcatcgttcc caaaatcacc
     2161 ctcacccctg ccctctcccg gcgtaccggg aaagggggac aaaacggttt agctcggcgc
     2221 ggcggtggtg ctctcaatca cgccttcggc gatcttcagt tcctctcgct tttgaatgcg
     2281 atccacccgg ccgtcgctga tccacaggat gcggtcgctg acggccagca tcttgtggtc
     2341 atgcgtcgcg ctgatgatcg tcacgccgcg ctcctggttc agttggcgca gcagctcgat
     2401 gatcaaccgg ccggtgtgca ggtcgaggtt tcccgtcggt tcgtcggcca ggatgatcgt
     2461 cgggtcattg gcgaacgcgc
//
LOCUS       DATIMH010000790         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2787503, whole genome shotgun sequence.
ACCESSION   DATIMH010000790 DATIMH010000000
VERSION     DATIMH010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2787503"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VLI90_13785"
     CDS             <1..872
                     /locus_tag="VLI90_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15325.1"
                     /translation="VVIPLAGHWVWFGWLKERGFIDVGGASVIHLSAAVCAAIGAALV
                     GPRMGKYNKDGSSNSVPGHALPLTSIGTLMLLVAWFPYLIGCVLAHDSSGDLSTVGFG
                     AVGMTATNIMLAAAGGALAGMIYGQFRYRKPDVSFTYIGLMGALVAISAGAAGMGNVG
                     ALITGAIAGLIVPLLTFEIDMRWHLDDPLGVAAIHGVGGLWGVFAAALFASGTVSERL
                     KLLGVQALGAAAIIALAAVVSLVVFLALKATLGLRVNDSDEFDGLDLGEHDINAYPDF
                     QQTTIKSYHLREA"
     gene            953..1792
                     /gene="panC"
                     /locus_tag="VLI90_13790"
     CDS             953..1792
                     /gene="panC"
                     /locus_tag="VLI90_13790"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HSV15326.1"
                     /translation="MKVLHTISEMRAERATLGPLALVPTMGALHAGHMSLIEVARTKA
                     PHVAVSIFVNPAQFGPREDFTKYPRPIVADLQKCEAAGVGVVFNPSEAEMYPGEGVDT
                     VIDFPQLTNVLEGKHRPGHFRGVCLIVAKLFNIIQPNIACFGQKDFQQLRVLNAMVES
                     LNWPIAMVGCPTLRDPDGMAMSSRNQYLSPQDRQRGLAISKALFAARDLVATGVKQAS
                     RLTTAMQHMLLAQHLLIDYIASVDPLTLKPVEIMTGPTVLAIAARVGATRLIDNIVVE
                     PKE"
     gene            1821..>2597
                     /locus_tag="VLI90_13795"
     CDS             1821..>2597
                     /locus_tag="VLI90_13795"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HSV15327.1"
                     /translation="MHPELFRIPLLNIPIFGYGLMMVLAFLATQWLSGRLAKTKGIDP
                     EIFVNVTLLALVAGVVGSRLSHVLENLPDYTRSDLSAWQNFVNMINIRSGGLTFYGGL
                     ILAFPVCLSYVLYKKVPPRIAMDIGAPCLMLGLAIGRIGCFLNGCCYGAETNVPWAVH
                     FPYGSNAYVDAFDRGEIDAPPQLKVRLTDGSYRLLSNEELQQGYIKTGDPQHPRVPIP
                     DDARQIAATQRSPAVHPAQLYSTFNALLITALLLAYFTMPH"
BASE COUNT          444 a          857 c          826 g          470 t
ORIGIN      
        1 cggtcgtcat tccgctggct gggcactggg tgtggttcgg gtggttgaag gaacgggggt
       61 tcatcgacgt cggcggcgcg agcgtgatcc acctgtccgc cgcggtgtgc gcggcgattg
      121 gcgcagcgct cgttggcccg cgcatgggga agtataacaa agatggctca tcgaattccg
      181 tgccggggca cgcgctgccg ctgaccagca tcggcacgct gatgctgctg gtggcgtggt
      241 tcccatatct gatcggctgc gtgctggcgc acgacagcag cggcgatctg agcacggtcg
      301 gtttcggcgc ggtcggcatg accgcgacga acatcatgct cgccgccgcc ggtggcgcgc
      361 tggccggcat gatttatggc cagttccgct atcgcaagcc cgacgtctcg ttcacctaca
      421 tcggcctgat gggcgcgctg gtggccatca gcgccggcgc cgccggcatg ggcaacgtcg
      481 gcgcgctgat caccggcgcg atcgccgggt tgatcgtgcc gctgctgacg tttgagatcg
      541 acatgcgctg gcacctggac gacccgctgg gcgtggcggc aatccacggc gtcggcgggc
      601 tgtggggcgt tttcgctgcc gcactgttcg ccagcggcac cgtgagcgag cgactgaagc
      661 tgctcggcgt gcaggcgctg ggcgccgccg cgatcatcgc gctggcggcc gtcgtatcgt
      721 tggtcgtgtt cctcgcgctc aaggccacgc tgggcctgcg cgtgaacgac tcggatgaat
      781 ttgacgggct ggacctgggc gagcacgaca tcaacgcgta cccggatttc cagcagacga
      841 cgatcaagag ttaccacttg cgcgaggcgt agcgtgaggc gtgaatacgt gattacgtga
      901 gcaaaagtca cataatcgcg taatcacgta ttcacgtaat cacataatca ctatgaaggt
      961 cctccacacg atttccgaaa tgcgagccga gcgcgcgacg cttggcccgc tcgcgctcgt
     1021 gcccaccatg ggcgcgctcc acgcgggcca catgtcgctg atcgaagtcg cgcgcaccaa
     1081 agccccgcac gtcgcggtgt cgatcttcgt caaccccgcg cagtttggcc cgcgcgagga
     1141 cttcaccaag tacccgcggc cgatcgtcgc cgaccttcaa aaatgcgaag ccgccggcgt
     1201 gggggtcgtg ttcaatccgt ccgaagcgga gatgtacccg ggcgaaggcg tcgataccgt
     1261 catcgacttc ccgcagctga ccaacgtgct ggaaggcaag catcgccccg ggcatttccg
     1321 cggggtttgc ctgatcgtcg ccaagctgtt caacatcatc cagccgaaca tcgcgtgctt
     1381 cgggcaaaaa gattttcagc aacttcgcgt gctcaacgcg atggtggaat cgttgaactg
     1441 gccgatcgcg atggtcggct gtcccacgct gcgcgacccc gacggcatgg cgatgagcag
     1501 ccgcaatcag tatctgtcgc cgcaggaccg ccagcgcggg ctggcgatca gcaaagcact
     1561 gttcgccgcg cgcgatctgg tggcgaccgg cgtgaagcag gccagccggc tgacgaccgc
     1621 gatgcagcac atgctgctgg cgcagcacct gctgatcgac tacatcgcgt cggtcgatcc
     1681 gctgacgctg aagccggtcg agatcatgac cggccccacc gtgctcgcca tcgcggcccg
     1741 cgtgggggcg acgcggttga tcgataacat tgtcgtcgag cccaaggagt agccgcggcc
     1801 cgcgcgccta cgattccacg atgcatcccg aactcttccg cattccgctg ctcaacatcc
     1861 cgatcttcgg atacgggctg atgatggtct tggcgttcct cgccacgcaa tggctgtcgg
     1921 gccgactcgc caagaccaaa ggcatcgacc ccgagatctt cgtcaacgtc acgctgctgg
     1981 cgctcgtcgc cggcgtggtc ggctcgcgcc tctcgcacgt gctggaaaac ctgccggact
     2041 acacgcgctc ggacctgagc gcctggcaaa acttcgtcaa catgatcaac atccgcagcg
     2101 gcgggctcac gttctatggc gggttgatcc tggcgttccc ggtgtgcttg agctacgtgc
     2161 tgtacaaaaa ggtcccgccg cgcatcgcga tggacatcgg cgcgccgtgc ctcatgctcg
     2221 gcttggccat cggtcgcatc ggctgtttcc tgaacggctg ctgctacggc gccgagacga
     2281 acgtcccgtg ggcggtgcat tttccctacg gcagcaacgc gtacgtcgat gccttcgacc
     2341 gcggcgagat cgacgccccg ccgcagctga aggtgcgcct gaccgacggc agctatcgcc
     2401 tgctctccaa cgaggaactt cagcaaggct acatcaagac cggcgatccg cagcacccgc
     2461 gggtgccgat cccggacgac gcccggcaga tcgccgccac ccaacgttcg cccgcggtgc
     2521 atccagcgca gctctacagc acattcaacg cgctgctgat cacggcgttg ctgctggcat
     2581 acttcaccat gccgcac
//
LOCUS       DATIMH010000791         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5391582, whole genome shotgun sequence.
ACCESSION   DATIMH010000791 DATIMH010000000
VERSION     DATIMH010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5391582"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..2241)
                     /gene="dxs"
                     /locus_tag="VLI90_13800"
     CDS             complement(196..2241)
                     /gene="dxs"
                     /locus_tag="VLI90_13800"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HSV15328.1"
                     /translation="MALGKERRQDDHQQQQRAIDRLLDRMHSPADLKKLSIDELTHLA
                     SEMRERIFAAVSANGGHLASNLGVVELTLALHYVFDFGPYPTGPDRLLFDVGHQSYPH
                     KMITGRVGQFEKLRKKGSVSGFPSPDESPYDLFAVGHAGTAISTACGMARGDQAMGRK
                     NHVVAVVGDASIVNGLSFEGLNGAGMVKRQLLLVLNDNGMSISNPQGAFAQYLERIRV
                     STTYEEFKRVSEKFVHRLPTNIGHTVEQAWNAICDGVKGVMWPGQIFESLGIKYMGPF
                     DGHDLPGLINFLSEIKHVEKPVLLHVKTKKGHGYELTKNEPTKFHSPAAFNVVGGMGQ
                     LVQNGCRVEMKKGDGKSWTTAFADACIELANKDPRVVTLTAAMPDGTGLSKFEKVHPT
                     RYFDTGICESHLTAMAAGMAKAGQRPIAAIYSTFIQRAFDQVWQEVVLNHQPVIFALD
                     RAGFVGDDGAVHHGFCDQSFLRPLPGMVLMAPTDEAELNRCLRFALSLETASALRYPR
                     DNVPACNFEDEIAVDLRDQAKQEWTLGQSRVLREGSDATIIVYGALAESAMTAADMLA
                     QDAIQIEIVDARFCKPIDGQMLARVLELGRPVLTVEDHSLQNGFGTAVAEYAIAHNLP
                     TANLVRLGMPDRMIAHATRKEQLTEVGLDPAGIAASVRDAIKTAASVEPNESALTRA"
BASE COUNT          523 a          837 c          884 g          548 t
ORIGIN      
        1 gtgggggtga tcatgttgcg gaaggacaag aatgaggcga agttcatgtg tgcgtctcct
       61 tgaaagagtt gaggtggatg cacgttttaa aagaagaagc cgcggatgta catcagatgt
      121 acatccgcgg ctttgaagtc ttatgcggga ggagctaacc gctgaagcgg ttagcccggt
      181 gaaggcgtcg ccagatcagg cgcgggtgag ggcggattcg ttcggctcga cgctcgcggc
      241 ggtcttgatc gcatcgcgga cgctggcggc gatgccggct gggtctaaac ctacttccgt
      301 tagctgctcc ttgcgcgttg cgtgggcgat catccgatcg ggcatgccca ggcgaaccag
      361 gttcgccgtc ggcaggttgt gagcgatcgc atattccgcg acggcggtgc cgaagccgtt
      421 ctgcaggctg tggtcttcca ccgtcaacac cggccggcca agttccagca cgcgggcgag
      481 catctggccg tcgatcggtt tgcagaagcg ggcgtcgacg atctcgatct ggatggcgtc
      541 ttgcgccagc atatcggcgg ccgtcatcgc gctttcggcc agcgcgccgt agacgatgat
      601 cgtggcatcg ctgccttcgc gcagcacgcg gctttggccg agcgtccact cttgctttgc
      661 ttggtcgcgc aggtcaaccg cgatctcgtc ttcgaaattg cacgccggca cgttatcgcg
      721 cgggtagcgc agggcgctgg ccgtctccag cgacagcgcg aaccgcaggc agcggttcag
      781 ctccgcttca tcggtcgggg ccatcagcac catgccgggc agcgggcgaa ggaacgattg
      841 atcgcagaag ccgtgatgca ccgccccgtc gtcgccgacg aaaccggccc ggtcaagcgc
      901 gaagatgacc ggctgatggt tcagcaccac ttcctgccag acctggtcga aggcgcgctg
      961 gatgaatgtc gaataaatgg cggcgatagg ccgctggccg gccttggcca tcccggcggc
     1021 catcgcggtc aggtggcttt cgcagatgcc ggtgtcgaaa tagcgcgtcg ggtgaacttt
     1081 ttcgaatttg ctcaggcccg tgccatcggg catggcggcc gtcagcgtga cgacgcgcgg
     1141 atctttgttg gccagttcga tgcacgcatc ggcgaaggcg gtcgtccagc tcttgccgtc
     1201 gcccttcttc atttcgacgc ggcaaccgtt ctgcaccagc tggcccatcc caccgacgac
     1261 gttgaacgcc gcggggctgt ggaacttggt cggctcgttc ttggtcagtt cgtagccatg
     1321 acccttcttg gtcttcacgt gcagcagcac gggcttttcc acgtgtttga tttccgagag
     1381 gaaattgatc agcccgggca ggtcgtgccc gtcgaacggg cccatgtact tgatgccgag
     1441 cgactcgaaa atctgccccg gccacatcac gcccttcacg ccgtcgcaga tcgcgttcca
     1501 ggcttgctcg accgtgtgac cgatgttggt cggcaggcga tgcacgaact tttcgctgac
     1561 gcgcttgaat tcttcgtaag tggtcgagac gcggatgcgc tccaggtact gcgcgaacgc
     1621 gccttgagga ttgctgatgc tcatcccgtt gtcgttcagc acgagcaaca gttgacgttt
     1681 caccattccg gcgccgttta gcccttcgaa cgacaggcca ttgacgatcg acgcatcgcc
     1741 gacgacggcg acgacatggt tcttccgccc catcgcctgg tcgccccggg ccatgccaca
     1801 ggcggtgctg atggcggtgc cggcgtgacc gacggcgaac aggtcgtacg ggctttcatc
     1861 cggcgaaggg aaaccgctga ccgagccttt cttgcgcagc ttttcgaact gcccgacgcg
     1921 gccggtgatc atcttgtgcg gataggactg gtgaccgacg tcaaagagaa gccggtccgg
     1981 cccggtggga taagggccga agtcgaagac gtaatgcaaa gcgagagtca gttcgacgac
     2041 gcctagattg gaagccagat gaccgccgtt ggcgctgacg gcggcaaaga tgcgctcgcg
     2101 catctcgctg gccaggtggg tcagctcgtc gatcgacagc ttctttaagt ccgcgggcga
     2161 gtgcatgcga tcgagcaatc gatcgatcgc gcgttgctgc tgctgatggt catcctgccg
     2221 acgttccttg cctagtgcca tctgccgaac ctcccgaggg aacacaagta gcgtatggcc
     2281 cctcgtcgga tcgcgtccgt gcgaagtcac gaaggcacgc aagaattaac ctgcgtgaaa
     2341 gttgccatct gcgagccgca tgcccaccgc cgccgagaag gctgcattgg aaacacaagg
     2401 ggccaaaccc gcaaggcggg tattatggcg aacaaatgcc gcttgtcacg gggatcggcg
     2461 gttgacaaca gccatgaacg ccgcaacatc tttcaaatac aagcgatacg acggccgccg
     2521 aggttggttc agccgcggcc gattgcctcg attccgggcg aattaccaaa acccttgccc
     2581 gctcgcgcgg cattcctaca atgccgcgtc tttcccacac agcgagcaac catgatcgat
     2641 tcgtacattc aaaatctgtt tgccgagcgc atcggcggca agggttacgg caagggcacc
     2701 gagatctaca agttcgagaa gatcaagcgg gccaagcgga aggcgctcag cgagcacccc
     2761 gagcggaagc tcctcgactt cggcatcggc ga
//
LOCUS       DATIMH010000792         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1926773, whole genome shotgun sequence.
ACCESSION   DATIMH010000792 DATIMH010000000
VERSION     DATIMH010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926773"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VLI90_13805"
     CDS             <1..956
                     /locus_tag="VLI90_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HSV15329.1"
                     /translation="ERAKAGVQVHVLIDWFGSEQIDPNYIKQMRSCGCEVHEYHAFHP
                     LDPASWGQIDHRTHRKILVVDGRIGFTGGVGIADEWRGNADAPDHWRDSHYHIEGPVV
                     SQLQGVFMDNWMQSTGRVLQGNDYFPKQPNDGKAFAQVFKSSSLGGSENIQLMVLLSI
                     AAAGQNIRLESAYFVPDKLTVSALIAARKRDVRVEIIVPGGKIDEHVVRQASRARWGD
                     LLKSGVEIYEYQPTMFHCKQMIIDNRWVSIGSANMDNRSFRLNDEANLNVLDEDFAAD
                     QIHLFEEDKQRSHRVTYEAWVHRPLGEKISNHLSSLLSWEL"
     gene            1020..1931
                     /gene="cysD"
                     /locus_tag="VLI90_13810"
     CDS             1020..1931
                     /gene="cysD"
                     /locus_tag="VLI90_13810"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HSV15330.1"
                     /translation="MSSSYNLTHLKQLEAESIHIIREVAAEFQNPVMLYSIGKDSSVM
                     VRLAQKAFHPGRLPFPLMHVNTTWKFREMIAFRNRFCAENGFQLIEHINTDGVKDGVN
                     PFDYGSNKFTRIMKTDALKAGLNQYGFDAAFGGARRDEEKSRAKERVYSFRDRLHQWD
                     PKNQRPELWNLYNGKVNKGESIRVFPLSNWTELDVWQYIYLENIPIVPLYFAAPRPVV
                     ERDGTLIMVDDDRMRLEPGEKPQMKVVRFRTLGDYPLTGAVESNATTLPQIIEEMLVA
                     RNSERQGRMIDHDEAGSMEQKKREGYF"
     gene            2006..2422
                     /locus_tag="VLI90_13815"
     CDS             2006..2422
                     /locus_tag="VLI90_13815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HSV15331.1"
                     /translation="MPYEEEDPPFVEPDVELNALATAVIGAAIEVHRRFGPGLDEALY
                     QASLWAELRYRNIEFAQQVFFDVRYRDEVIGTRRIDLIVGNRLVVELKAVEQLSPLHM
                     AQVKTYLKITGLTLGLLINFNVILLKDGLRRIIYHH"
     gene            2480..>4205
                     /gene="cysN"
                     /locus_tag="VLI90_13820"
     CDS             2480..>4205
                     /gene="cysN"
                     /locus_tag="VLI90_13820"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HSV15332.1"
                     /translation="MQKLATLNEDIHTYLDRHQRKELLRFLTCGSVDDGKSTLIGRLL
                     HDTKMIYEDQLAAVKKDSAKFNTTGIAGEVDLALLTDGLKAEREQGITIDVAYRYFST
                     DKRKFIIADTPGHEQYTRNMATGASTCQLAIILIDARHGVVSQTKRHSFIVSLLGIKH
                     VVVAINKMDLVNYGQETFERIKADYTGFVAKLGIPDVQFIPLSALKGDNVVEKSPATP
                     WYQGPPLLNHLETVHIASDRNLTNMRFPVQYVIRPNLNFRGFAGTIASGIIRVGDEVV
                     ALPSGRRSKVRSIVTYDGELQEAFSPQAVTVTLADEIDVSRGDMLVHPGDQPYVSNQI
                     EAMVVWMHEKPFVPGKSYWIKHTTRTVTGEIAELRFAVDVNTLEKRPATQLAMNEVGH
                     VLLSLNQAVPFDPYKFNAATGAFIIIDRLTNNTVGAGMIQERMDRRGQGDIWNATPIA
                     GKLKQKKSKVTQAEREERLGHKAATVLIYGLTGSGKATIAYELEKRIWDAKCEVTVLY
                     GPDMRQGLCRDLGFTADDRSENLRRSAEVAKILNDAGAICVCAFVAPHDAVRQKARDV
                     IGGERFIEV"
BASE COUNT          909 a         1202 c         1323 g          771 t
ORIGIN      
        1 gcgagcgcgc caaagccggc gtgcaggtcc acgtgctgat cgactggttc ggctccgagc
       61 agatcgaccc gaattacatc aagcagatga gatcgtgcgg ctgcgaggtc cacgagtacc
      121 acgcgtttca cccgctcgat ccggcttcgt ggggacagat cgaccaccgc acgcaccgca
      181 agatcctggt cgtcgatggt cggatcggtt tcaccggcgg ggtgggcatc gcggacgagt
      241 ggcgcggcaa cgccgacgcg cccgaccatt ggcgcgattc gcattaccac atcgaagggc
      301 cggtcgtctc gcagctgcag ggcgtgttca tggacaactg gatgcagtcc accggccgcg
      361 tgctgcaggg caacgattat ttccccaagc aacccaacga cggcaaagcc tttgcgcagg
      421 tgttcaagag ttcatcgctg ggcggcagcg aaaacattca gctgatggtg ctgctgtcga
      481 tcgccgccgc ggggcagaac atccggctgg agagcgcgta tttcgttcca gacaaactga
      541 cggtcagtgc gctgatcgcc gcgcgcaaac gcgacgtgcg cgtcgaaatc atcgtccccg
      601 gcgggaagat cgacgagcac gtcgtccgcc aggcctcgcg ggcgcgatgg ggcgatctgt
      661 tgaaatctgg cgttgagatt tacgaatatc agccgacgat gttccactgc aagcagatga
      721 tcatcgacaa ccggtgggtg tcgatcggct cagccaacat ggacaaccgc tcgttccgcc
      781 tgaacgacga ggcgaacctc aatgtgctgg acgaagattt tgccgccgac cagattcact
      841 tgttcgagga agacaagcag cgctcgcacc gcgtgacgta cgaagcgtgg gtccaccgtc
      901 cgctggggga gaagatcagc aatcatttgt cgagcctgtt gtcgtgggag ttgtgattgc
      961 cgggggaaat ccgctactct ggggacgaac cgagttttca agattcccga gtacgcccga
     1021 tgagcagcag ttacaacctg acgcatctga agcagctgga agccgagagc atccacatca
     1081 ttcgcgaggt ggccgccgag ttccagaatc cggtcatgct ctattcgatc ggcaaggact
     1141 cttcggtgat ggtccgcctg gcgcagaagg cgttccaccc gggccggttg ccgtttccgc
     1201 tgatgcacgt gaataccacg tggaagttcc gcgagatgat cgcattccgc aatcgcttct
     1261 gcgccgagaa cggatttcag ctgattgagc acatcaacac cgacggtgtg aaggacggcg
     1321 tgaacccgtt cgactatggc tcgaacaagt tcacccgcat catgaagacc gatgcgctca
     1381 aggccggctt gaaccagtac ggtttcgacg ccgccttcgg cggggcgcgt cgcgacgaag
     1441 aaaagagccg ggcgaaagag cgcgtctatt ccttccgcga tcggctgcat cagtgggacc
     1501 cgaaaaacca gcggccggag ttgtggaacc tgtacaacgg caaggtgaac aaaggcgaat
     1561 cgatccgcgt cttcccgctg agcaactgga ccgagttgga tgtctggcaa tacatctatc
     1621 ttgagaacat cccgatcgtc ccgctgtact tcgccgcccc gcggccggtg gttgaacgcg
     1681 acggcacgct gatcatggtc gatgatgacc gcatgagatt ggagccgggc gagaagccgc
     1741 agatgaaggt cgtgcgcttc cgcacgctgg gcgactaccc gttgaccggc gcggttgaga
     1801 gcaatgcaac gacgttgccg cagatcatcg aggagatgct ggtcgcgcgc aatagtgagc
     1861 ggcagggccg gatgatcgat cacgatgaag cggggtcgat ggagcagaag aagcgggaag
     1921 ggtatttttg agtttgaaga gatgtgaacg cagagacgcg gaggccgcgg agctaggacc
     1981 ggagagggtg agagaggaag gatggatgcc ttacgaagaa gaggatccgc cgtttgttga
     2041 gcctgatgtt gagttgaacg cgctggcaac agccgtaatt ggcgcggcaa ttgaagtgca
     2101 tcgtcggttc ggtccaggtt tggacgaagc actatatcag gcatcattgt gggccgaatt
     2161 gcgctaccgg aacattgaat ttgcgcaaca ggttttcttt gacgtcagat accgcgacga
     2221 ggtgatagga accaggcgga ttgatctcat cgttggcaat cggctcgtgg tggaattgaa
     2281 ggcggtggaa cagttatcgc cgctgcatat ggcacaggtg aaaacgtacc tgaagatcac
     2341 cgggctgacg ctcggactgc tgatcaactt caacgtaatc ctgctcaagg acggccttcg
     2401 gcgaattatc tatcaccatt aaccttcttc tctggttcta cctccgcggc ctctgcgtct
     2461 ctgcgttcac ttaactatca tgcagaaatt ggcaacgctc aacgaagaca ttcacaccta
     2521 cctcgaccgc catcagcgga aggagttgct gcgctttctc acctgcggct ctgtcgatga
     2581 tggaaaatcc acgctcatcg gccgcctgct gcacgacacc aagatgatct acgaagatca
     2641 gctggcggcc gtgaagaaag acagcgcgaa gttcaacacc accggcatcg ctggcgaagt
     2701 ggatctggcg ttgctcaccg acgggttgaa agccgagcgc gagcagggga tcacgatcga
     2761 tgtggcgtat cgctactttt ccaccgataa gcgcaagttc atcatcgccg acacgccggg
     2821 gcacgagcag tacacgcgga acatggccac tggcgcgagc acctgccaac tggcgatcat
     2881 cctgatcgac gcccggcacg gcgtggtttc gcagaccaag cgccacagct tcatcgtctc
     2941 gctgctgggc atcaagcacg ttgtcgtcgc cattaacaag atggacctgg tcaactacgg
     3001 tcaggaaacg ttcgagcgga tcaaggccga ttacaccggc ttcgtcgcca agctcggcat
     3061 tccggacgtg cagttcattc cgctgtcagc gctcaagggc gacaacgtcg tcgaaaaatc
     3121 gccggccacg ccgtggtatc aggggccgcc cctgttgaac cacctggaga ccgtgcacat
     3181 cgcgtcggac cgcaacctga cgaacatgcg gtttcccgtg cagtatgtga tccgcccgaa
     3241 cctgaacttc cgcgggttcg ccggcacgat cgcatccggc atcatccgcg ttggcgacga
     3301 agtcgtcgcg ctgccgtcgg gccggcggag taaggtgcga tcgatcgtca cctacgacgg
     3361 cgaattgcag gaggcattta gcccgcaggc ggtgacggtg acgctggccg acgagatcga
     3421 cgtcagccgc ggcgacatgc tggtgcatcc cggcgatcag ccgtacgtgt ctaaccagat
     3481 cgaagcgatg gtcgtgtgga tgcacgaaaa accgttcgtg ccgggcaaga gttactggat
     3541 taagcacacg acgcgcacgg tgaccggcga gatcgccgag ctgcgcttcg cggttgacgt
     3601 caacacgctc gaaaagcgcc cggcgacgca gctggcgatg aacgaagtcg gccacgtgct
     3661 gctgagcctg aaccaggcgg ttccgttcga cccgtacaag ttcaacgcgg ccaccggcgc
     3721 gttcatcatc atcgaccgcc tgacgaacaa cacggtgggc gcggggatga tccaggagcg
     3781 gatggatcgc cgcgggcaag gcgacatctg gaacgcgacg ccgatcgccg ggaagttgaa
     3841 gcagaagaag agcaaggtga cgcaggccga gcgcgaggag cgcctcggcc acaaggccgc
     3901 gactgtgttg atctatggct tgaccggctc gggcaaagcg accatcgcgt acgaattaga
     3961 gaagcggatc tgggacgcga aatgtgaggt cactgtcctg tatggccccg atatgcggca
     4021 gggcctgtgc cgcgatctcg gtttcaccgc cgatgatcgt tcagaaaacc tgcgacgatc
     4081 cgccgaggtg gcgaagatcc tcaacgacgc cggcgcgatc tgcgtgtgcg cgttcgtcgc
     4141 cccgcacgat gccgtgcggc aaaaggcgcg cgacgtcatc ggcggcgagc gcttcattga
     4201 ggtgt
//
LOCUS       DATIMH010000793         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2499034, whole genome shotgun sequence.
ACCESSION   DATIMH010000793 DATIMH010000000
VERSION     DATIMH010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499034"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VLI90_13825"
     CDS             complement(<1..799)
                     /locus_tag="VLI90_13825"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HSV15333.1"
                     /translation="MSDNDGLSNATGGAAAPAEDYTVAHGLSRSLDYAHGTVLLPHRT
                     GSWWRQMFAFAGPAYMVAVGYMDPGNWATDIEGGARFGYRLIWVLLMSNLMAVLLQTL
                     AARLGVVAGRDLAQACREAYGGGVRWTLFILCEIAIAACDLAEVLGTAIGLQLLTSQV
                     FGGHGIPLMWAVLITGLDVFLLLAIQRFGIRKMEAFIISLVSIIGMCFIVEIFLSKPS
                     PAGIASGFIPTGLSHGMLYVAIGIIGATVMPHNLYLHSALVQSRDVPR"
     gene            1015..1683
                     /locus_tag="VLI90_13830"
     CDS             1015..1683
                     /locus_tag="VLI90_13830"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HSV15334.1"
                     /translation="MPSSTVEDYLKQIYLQQQAEPDQPVATGRLAEVMGVVPGTATSM
                     IKALADSGLVAYEPRGGTRLTRGGEQLALHVLRRHRLVELFLVKTLGLDWSEVHEEAE
                     ELEHAISDKVLQRIDELLGHPQVDPHGDPIPTAKGRVRDRARLQPLSACEPGSAITIA
                     RVIDQDPPFLQFINRCGLTPGVAATIEQRNPQADAVVVRVQRGKPLITLGNAAAAKIL
                     VEVA"
     gene            1888..2688
                     /locus_tag="VLI90_13835"
     CDS             1888..2688
                     /locus_tag="VLI90_13835"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HSV15335.1"
                     /translation="MTNSSDQVAQYRRDGFIQLRDVFTSDALQHLRDAVAGAVQLEQQ
                     HDWLGRRRTAASEPKNAYEQIFIQKVNLWTRHADVKSFVFSQRLGHIAATLGGHPMRL
                     WHDQALFKEPRTGTATPWHQDAVYWPHRPRRDQLTIWIALRDATIANGCMSFIPGSHA
                     LGPLPPINLAHPQDLFAHAPQMQPVEPVTFELKAGSVTVHNGLTFHCAGPNTADQMRE
                     ALAIIYMPQDTSFDGGSHPVTDPLKSRGLKEGDPLNDGELFPIVGTLS"
     gene            complement(2820..3362)
                     /locus_tag="VLI90_13840"
     CDS             complement(2820..3362)
                     /locus_tag="VLI90_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HSV15336.1"
                     /translation="MKSTKGKFLIASPKLTDPNFAQAIVLMVQHDEKGALGVVINRPL
                     EVTVQQACEQVLGTGCEVGGVLHHGGPCEALLMVIHTDEDNGEAEVIPGVYFTTAKES
                     LELLLDNPADDMRFFVGYSGWGPGQLEAEFETGSWTTTPATAERIFGPVENLWTRLNT
                     EVNLSKWVHPTRIPEDPSVN"
BASE COUNT          689 a         1227 c         1053 g          598 t
ORIGIN      
        1 tccgcggcac gtcccggctc tgcaccagcg ctgaatgcag gtacaaatta tgcggcatca
       61 ccgtggcgcc gatgatgccg atcgccacgt acagcatccc gtggctcagc cctgtcggaa
      121 tgaatccgct ggcgatcccc gccggcgacg gtttgctcag gaagatctca acgatgaagc
      181 acatgccgat gatcgacacc agcgagatga tgaaggcctc catcttgcgg atgccgaaac
      241 gctgaatcgc cagcagcagg aagacatcca ggcccgtgat cagcaccgcc cacatcagcg
      301 gaatgccgtg cccgccgaag acttggctgg tgagcagctg aaggccaatc gcggtgccga
      361 gcacctcggc caggtcgcac gcggcgatcg cgatttcgca caggatgaac agcgtccacc
      421 gcaccccgcc gccgtacgct tcgcgacagg cttgcgccaa gtcgcgcccc gcgacgacgc
      481 ccagccgcgc cgccagcgtc tgcagcagca cggccatcag gttgctcatc agcaaaaccc
      541 agatgagccg ataaccgaag cgcgccccgc cttcgatgtc ggtggcccag ttgcccgggt
      601 ccatgtagcc cacggcgacc atgtacgccg gccccgcgaa ggcgaacatt tgccgccacc
      661 aggacccggt gcgatgcggg agcaggacgg tgccgtgcgc gtaatcaagc gatcgcgaca
      721 agccgtgagc gacagtgtaa tcctccgccg gcgccgcagc gccgccggtg gcattgctca
      781 acccgtcgtt gtcgctcatt tcgcgattca tcgtggcccg accaaaccat cacctgacca
      841 tccccacgcc gggttcggag cgtactcgcg acgacccgga ttcttctgct cgacgcgcgg
      901 cagaatccgg gtcgtcggag tacctccgaa cccggcgtgt gggagcaacc tgattttgat
      961 gactcaaaat atgattgcat aaccgcccgt tctttgtcaa gctatgatcc caacatgccc
     1021 agcagcaccg tcgaagatta cctcaagcag atctacctgc agcagcaggc ggagcccgac
     1081 caaccggtcg ccaccggccg cctggccgaa gtcatgggcg tcgtccccgg caccgccacc
     1141 agcatgatca aagccctcgc cgactccggc ctggtcgcct acgaaccccg cggcggcacc
     1201 cgcctcaccc gcgggggcga gcagctggcc ctgcacgtcc tccgccgcca tcggctggtc
     1261 gaactgttcc tcgtcaaaac actcggactt gattggtccg aagtacacga agaagccgaa
     1321 gaactggaac acgcgatttc cgacaaggtg ctgcaacgga tcgacgagtt actcggccat
     1381 cctcaggtcg atccacacgg cgatccgatc cccacggcca aaggccgcgt gcgcgatcgc
     1441 gcgcgcctgc aaccgctcag cgcctgcgag cccggctcag ccatcacgat cgcccgcgtg
     1501 atcgaccagg acccgccgtt cctgcagttc atcaaccgct gcggcctgac gcccggcgtc
     1561 gcggcgacga tcgagcagcg caacccgcaa gcggatgccg ttgtcgtgcg cgttcaacga
     1621 gggaaaccgc tcatcacgtt ggggaacgct gccgcggcga agattctggt ggaggtcgcg
     1681 taatactcgt agctgcgacg ccagcgtcgc ctcttcggct cggaaaaaca aggcgacgca
     1741 ggcgtcgcag ctacagcgca gtcgagtatt tttagaagct ttaatagccc aggccgggag
     1801 gccgaggatc gatcgtcgca tcggcgaggt ttcaaatccg atcccccgcc ttccggcctg
     1861 ggctattatt cgttcgtgca gaacgacgtg actaattctt ccgatcaagt cgcgcaatat
     1921 cgccgcgatg gattcattca gcttcgcgat gtcttcacga gcgatgcgct gcaacacctg
     1981 cgcgatgccg tcgccggcgc cgtgcagctg gaacagcagc acgactggct gggccgccga
     2041 cgcaccgcag cctcagaacc aaagaacgcg tacgagcaga tcttcatcca gaaggtgaac
     2101 ctctggacgc gacacgccga cgtgaaatcc ttcgtgttct cccagcgcct cggccacatc
     2161 gccgcaacgc tcggtggtca tcccatgcgc ctgtggcacg atcaagcgct gttcaaagag
     2221 ccgcgcaccg gcacggcgac gccgtggcat caggacgcgg tctattggcc gcaccgcccg
     2281 cggcgggatc agctgaccat ctggatcgcc ctgcgcgacg ccacgatcgc caacgggtgc
     2341 atgtcgttta ttccgggatc gcatgcgctc ggcccactgc cgccgatcaa cctcgctcat
     2401 ccgcaggacc tgttcgcgca cgcgccgcaa atgcagccag tcgagccagt gacgtttgaa
     2461 ctgaaagccg gcagcgtcac ggtgcacaat ggcctgacct tccactgcgc cgggccgaac
     2521 acggcggacc agatgcgcga agcgctggcg atcatctata tgccgcaaga cacgagcttc
     2581 gatggcggca gccatccggt gacggacccg ctcaagtcgc gcggattgaa ggaaggcgat
     2641 cccctgaacg acggcgagtt gttcccgatc gtgggaacgc tttcgtagcc gcaggcgaca
     2701 gccagcgggg gccccttcga caatcaaccc ggccggtgct cgagcgcccc cgctggctgt
     2761 cgcctgcggc tattaaatgc agtcatcatt gcccacaccc gcacgtcaac gcacttgagt
     2821 caattcaccg acggatcctc cggaatgcgc gtcggatgca cccacttgct caggttcact
     2881 tccgtgttca gccgcgtcca gaggttttca acggggccga agatgcgctc cgccgtcgcc
     2941 ggcgttgtgg tccacgaacc ggtttcgaac tccgcttcca gctgccccgg tccccagccg
     3001 gagtagccga cgaagaagcg catgtcgtcg gccgggttgt ccagcagcag ctcgaggctt
     3061 tccttggcgg tcgtgaaata gacgccgggg atgacctcgg cttcgccgtt gtcctcgtcg
     3121 gtgtgaatga ccatcagcag cgcttcgcat gggccgccgt ggtgcagcac gccgcccact
     3181 tcgcaaccgg tgcccagcac ctgctcgcac gcctgctgca cggtcacttc cagcgggcgg
     3241 ttgatgacga cgccgagcgc gcccttctcg tcgtgctgca ccatcaacac gatggcctgc
     3301 gcgaagttcg ggtccgtcaa cttcggcgag gcgatcagga atttaccctt ggtcgatttc
     3361 atctcaattc gaccgacaat agccgcggcg ttggtggatg caaacccgaa actttgcagc
     3421 ggcgcgcggg catcagggaa acgtgcgcgt ggcctctttt ccccctctcc cggggtaccg
     3481 ggagagggga cagaaggcgc cgttgccttg gcggcgtcac ctcaagatcc agactagcag
     3541 cacgatcaac acgcagacga tggcgcc
//
LOCUS       DATIMH010000794         1770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2499203, whole genome shotgun sequence.
ACCESSION   DATIMH010000794 DATIMH010000000
VERSION     DATIMH010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499203"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VLI90_13845"
     CDS             <1..615
                     /locus_tag="VLI90_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HSV15337.1"
                     /translation="VMQVRAIIEAACEVKKQKIKVLPEIMIPLVGTKAELDYLKKYTV
                     ETANAVMKEQGVKVDYMYGTMIEVPRAAVTADEIAETAEFFSFGTNDLTQMTFGYSRD
                     DVNSFLPDYLSKEILERDPFQSLDTEGVGQLIKMAVQKGRATNKDLKCGICGEHGGDP
                     RSVEFCHNVGLNYVSCSPYRVPIARLAAAHAAILAKGGKVQSTK"
     gene            897..1613
                     /locus_tag="VLI90_13850"
     CDS             897..1613
                     /locus_tag="VLI90_13850"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HSV15338.1"
                     /translation="MCGRYTLVRLADFLSRIPWIAPPDDAAPASPPRYNVAPTQAVAA
                     VTNARPAKVEFLRWGLVPSWAKDLSVGNRMINARAETLAEKPAFARLLRRRRCLVPAD
                     GFYEWRKEAGGRIKTPMYIRMADGHPFAFAGLWDTWRDSSGQTVRSCTIITTTPNELV
                     RSIHDRMPAILREADYERWLQPEAPPDDTPPAAQLEMLRPYPPEEMEAFAVSSSVNNP
                     RTESPLNICPAKDGLWDSPP"
BASE COUNT          342 a          536 c          582 g          310 t
ORIGIN      
        1 gtgatgcagg tgcgggcgat catcgaggcg gcctgcgaag taaagaagca gaaaatcaag
       61 gttctgccgg agatcatgat cccgctggtc ggcaccaagg ccgagctgga ttacctgaag
      121 aagtacaccg tcgaaaccgc caatgctgtg atgaaggagc agggcgtgaa ggtggactat
      181 atgtacggca cgatgatcga agtgccgcgc gccgcggtga cggccgacga gatcgccgag
      241 accgccgagt tcttcagctt cggcaccaac gacttgacgc agatgacgtt cggctacagc
      301 cgcgacgatg tgaacagctt cctgcccgat tacctgagca aggaaatcct ggagcgcgac
      361 ccgttccaat cgctggacac cgaaggcgtc gggcagttga tcaagatggc tgtgcagaag
      421 ggacgcgcga cgaacaagga cctcaagtgc ggcatctgcg gtgaacacgg cggcgacccg
      481 cgctcggtcg agttctgcca caacgtcggt ttgaactacg tcagctgctc gccgtaccgc
      541 gtgccgatcg cacgcctggc cgcggcccac gccgccattc tcgccaaggg cgggaaggtg
      601 cagtcgacga agtaaggttt cagcttcgcg ccaattcttc tgaagcaaga cgcggccgat
      661 ccagcgatgg gtcggccgcg tttgtctttg agttcaagct gagcggagca cgtacccatg
      721 ccgagtcgaa ggatctggcc gcgacgtagg tgaaccgaca ctcgcgcaat tcccgccgct
      781 tgcggcttag cgtcttttcg accagaagag aacgcacgct aagccgcaag cggctgtcgc
      841 gcgcatgacg aacagcgatc cacaaaaaaa atacgcggct atgattaggg gaacccatgt
      901 gcggtcgcta taccctcgtt cgccttgccg attttctcag ccgcatcccg tggattgcgc
      961 cgccggatga tgctgcgccg gcttcgccgc cgcggtacaa cgtcgcgccg acacaggcgg
     1021 tggccgccgt gaccaacgct cggccggcga aggtggagtt tttgcgatgg ggcctggtgc
     1081 cgtcgtgggc caaggatctg tcggttggca atcgcatgat caacgcgcgg gcggagacat
     1141 tggctgagaa gcccgcgttt gcgcggctgc tgcgccggcg gcgctgtctc gtgcccgccg
     1201 atgggtttta tgagtggcga aaggaagccg gcggaaggat caagacgccg atgtacatcc
     1261 gcatggccga cgggcatccc ttcgccttcg cggggctgtg ggatacgtgg cgcgattcat
     1321 ctggccagac cgtgcggtcc tgcacgatca tcacgaccac gccgaacgaa ctggtgcggt
     1381 cgatccacga ccgcatgccg gccatcttgc gcgaagcgga ttacgagcgc tggctgcaac
     1441 cggaggcgcc gccggacgac accccgccgg cggcgcagtt ggagatgctg cggccttacc
     1501 cacctgagga gatggaagcg ttcgcggtgt ccagcagcgt gaacaacccg cgcaccgaat
     1561 cgccgctcaa catctgcccg gcgaaggacg gcctgtggga ttcgcctccg tgagacgctg
     1621 tcgccgttcc catagatggg tttgatcctg ctcaatttcg cgatcggcgc tgcggtcgcc
     1681 gcgctgctct ggttcatcgc cgggcggtgg atcgtgatgc tgctcgacca cgtatttcgc
     1741 gggcgcgaag agacgatcgc agccgagccg
//
LOCUS       DATIMH010000795         1718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_480034, whole genome shotgun sequence.
ACCESSION   DATIMH010000795 DATIMH010000000
VERSION     DATIMH010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480034"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VLI90_13855"
     CDS             complement(<1..744)
                     /locus_tag="VLI90_13855"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HSV15339.1"
                     /translation="MNQVPAIDPGKKSRIDAILKNAIDPHVHSGPSIAARAVDHLELV
                     KQLSEAGFAAVVTKDHDYAGVATAALIAKHHPELRTKIFSGIALNNVVGGMNPYAVEH
                     TAAMGGKIVWMPTLAAENHLRWEKNASFVHPASTTKMRPASAVPVLDANKKVRDDVKD
                     VLDVIARNDMVLASGHLHVSETWIVFEEAQRRGVKRLIFTHPEDIVDASLNDVKGIAA
                     MGAQVEHSLCMFLDGSKFKTRTADDLRNQI"
     gene            complement(778..1503)
                     /locus_tag="VLI90_13860"
     CDS             complement(778..1503)
                     /locus_tag="VLI90_13860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSV15340.1"
                     /translation="MHNWRVLIVHAHPEPASFNAALTGAAAEALRGAGHEVTVSDLYA
                     DGFEGMAGRGDFTTTADASRFHYQSEQAYAAAHDGFAATIRREQERLRQADLLILQFP
                     LWWGAPPAILKSWFERVLAYGFAYVDAHRFDTGLFRGRRALIGVTTGGTPARFAPDGV
                     YGEIERVLWPVRRLTLEYMGFEVDEPFVAYGVPRVDDAEREKYLAAWRQRVLAATSHE
                     PPQTRVDPLAALAAVGASAWARS"
     gene            complement(1496..>1718)
                     /locus_tag="VLI90_13865"
     CDS             complement(1496..>1718)
                     /locus_tag="VLI90_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15341.1"
                     /translation="PWTTNLNNWREDQHEYCGSPSVTVIPPPSIHTSRGMAEGINQLV
                     DIFSPPRKDFSLKPGWVLNAADYPMPSDA"
BASE COUNT          279 a          550 c          577 g          312 t
ORIGIN      
        1 gatctggttg cgcaggtcat cggcggtgcg ggtcttgaac ttggatccgt cgaggaacat
       61 gcagagcgag tgctcgacct gcgcgcccat ggcggcgatg cccttgacgt cattgaggct
      121 ggcatcgacg atgtcttccg gatgggtgaa aatcagccgc ttgacgccgc ggcgctgcgc
      181 ctcctcgaac acgatccagg tctcgctgac atggaggtgg ccgctggcca gcaccatgtc
      241 gttcctggcg atcacgtcca gcacgtcctt gacgtcgtcg cgaaccttct tgttggcatc
      301 gagcaccggc accgcagacg cggggcgcat cttcgtggtc gaggccgggt ggacgaagct
      361 cgcgttcttt tcccaccgca gatggttttc cgccgccagc gtcggcatcc agacgatctt
      421 gccgcccatc gccgcggtat gctcgacggc gtatgggttc atgccgccca ccacgttgtt
      481 gagcgcgatc cctgagaaga tcttcgtgcg caactccgga tggtgtttgg cgatcagcgc
      541 ggctgtggcg acgccggcgt agtcgtggtc cttggtgacg acggcggcaa atccggcctc
      601 cgacagctgc ttcaccagtt cgagatgatc gaccgcgcgc gccgcgatcg atggcccgct
      661 gtggacatgc ggatcgatgg cgttcttgag gatggcgtcg atgcggctct tcttgccggg
      721 gtcgatggcg ggtacctggt tcatgatggc ctctctttcg gagttcggat ctggttgtca
      781 gcttcgcgcc caggccgacg ccccgacggc ggccagggcg gcaagcgggt cgacgcgcgt
      841 ctgcggcggc tcgtgcgagg tggcagcgag cacgcgctgc cgccaggcgg caagatattt
      901 ttcacgttcg gcgtcatcga cgcggggaac gccataggcc acgaacggct cgtcgacctc
      961 aaaacccata tactcaaggg tcaggcgccg caccggccat agcacccgct cgatctcgcc
     1021 gtacacgccg tccggcgcga accgcgccgg cgtgccgccg gtggtcacgc cgatcagcgc
     1081 gcggcgcccg cgaaacaggc cggtatcgaa acggtgggcg tcgacatagg cgaagccata
     1141 ggccagcacc cgttcgaacc agctcttcag gatggccggc ggcgcgcccc accacagcgg
     1201 aaactgcagg atgagcagat ccgcctggcg cagccgttcc tgctcgcgcc tgatcgtggc
     1261 ggcgaagcca tcatgcgcgg cagcgtaagc ctgctcggac tgatagtgga agcgcgaagc
     1321 atcggccgtg gtggtgaagt cgccgcggcc cgccatgccc tcgaaaccgt cggcataaag
     1381 gtcggagacc gtgacttcgt gtccggcgcc gcgcaacgcc tccgccgccg cgccggtcag
     1441 cgcggcgttg aacgacgccg gttcggggtg ggcatgaacg atcagtactc gccagttatg
     1501 catctgatgg catcgggtaa tccgccgcat tcagaaccca gccgggcttg agcgaaaagt
     1561 ccttccgcgg cgggctgaag atgtcgacca gctgattgat gccctcggcc atgccgcgcg
     1621 aggtatggat cgatggcggc gggatcaccg tgaccgacgg cgacccgcaa tattcgtgct
     1681 ggtcttcccg ccagttgttc aggttggtgg tccacggc
//
LOCUS       DATIMH010000796         4077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5104443, whole genome shotgun sequence.
ACCESSION   DATIMH010000796 DATIMH010000000
VERSION     DATIMH010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5104443"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1890
                     /locus_tag="VLI90_13870"
     CDS             76..1890
                     /locus_tag="VLI90_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSV15342.1"
                     /translation="MPTFKRADQEQFERAKDLIETEPKELGFVKSMFFGRLKPDQVLP
                     YPQQDADEARRTDELIARLDEFLKREVDADRIDAEERIPPNVIEGLGKLGVLGMTVPK
                     EFGGGGFSHTAYCRVLEHVARHCTSTAVFIGAHQSIGLKALLLMGTEEQKRQYLPALA
                     SGEKIAAFCLSEPQVGSDAANVQTTARLSDDGSHWILNGEKKYATNAALAGFMTVMAK
                     TPVTDERGKTRDKVTAFIVTPDLPGFEVVKSNRSKCGIRGSWQAVLRFTDMRVPSDRI
                     LGGLGKGLKVALSVLNYGRCTMSAGCVGGAKLALEMCIDRARTRKQFGRAIGEFHLVK
                     EKLARMAESVFAMDAMTYLAAGLVDRHVDDLMLETAICKLFCSEAAWQVIDDAVQLWG
                     GEGYMRETGLERMLRDARINRIVEGTSEVMTAFISLVGMKGVGEDLEQVMRLAKHPVG
                     NFGRLATFARGEWSDVVMGHSLEGLHPKLSNEGRTFARLTKLLARDVVRLLGKHRERI
                     LDMELVHQRVTGAAMELYAMAAVISRMQMLIERSERATEGNGHDKLRRDLTVGRSFSR
                     HAAQRVERHLDSLFSNQDEDVLQTANAVLGWECDPSAE"
     gene            complement(2172..3317)
                     /locus_tag="VLI90_13875"
     CDS             complement(2172..3317)
                     /locus_tag="VLI90_13875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSV15343.1"
                     /translation="MTTTNPATPASAPSYSPGLAGVIAGESAISAVDGESGLLYRGYD
                     IHELAVRASFEEIIYLLLHGELPDTSQLEKTREELAADAPLPGAVRSMLKLMPPRAHP
                     IDVLRTGVSMLASFDPELPDNSHAANLRKAMRIIARTSTIITASHRIVSGQEPYTPGE
                     HLTFAARFLFGMSGKEPEPWRVGAMNLIFTLYAEHEFNASTFAARVTASTLSDIYAGV
                     VSGLGTLKGPLHGGANEEAMKMLKEIGTPDYAEEWVKKHLAHKDKIMGFGHRVYKKGD
                     SRVPAMRDLARQLGRRLGHEQLVDVCVKLEEVMAKEKGLYANLDLYAAPALHLMGIPP
                     ELDVPVFAAARVAGWCAHIIEQHDHNRLIRPRSLYNGPAKRAYPTAH"
     gene            complement(3650..>4077)
                     /locus_tag="VLI90_13880"
     CDS             complement(3650..>4077)
                     /locus_tag="VLI90_13880"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HSV15344.1"
                     /translation="RDGDFLIVARTDARADEGIDGAIERAIAYVKAGADAIFPEALQS
                     PAEFRQFAKAVNVPLLANMTEFGRSPLLSVRQLAAMGYRMVIFPQSAFRASMRTSLEL
                     LNDLKRRGTQQQWLDRMQTRQELYDLLDYDPAAEDWPRR"
BASE COUNT          749 a         1288 c         1299 g          741 t
ORIGIN      
        1 tcgaacgacc gcggacagtg aagtcgtgca aaaatgactg catgggccgg ctgcccatgc
       61 cacgagagga aacctatgcc gacattcaaa cgagccgacc aggagcagtt cgagcgggcc
      121 aaggacctga tcgagaccga gccgaaggag ctgggtttcg tgaagtcgat gttcttcggc
      181 cggcttaagc ccgatcaagt cctgccgtat ccgcagcagg atgccgacga ggcgcgccgc
      241 acggacgagc tgatcgcccg gctcgatgag tttctcaagc gcgaagtgga cgccgaccgg
      301 atcgacgccg aagaacgcat cccgccgaac gtcatcgaag gcctgggcaa gctgggcgtg
      361 ctcggcatga cggtgccgaa ggaattcggc ggcggcgggt tctcgcacac cgcgtattgc
      421 cgggtgctcg aacacgtcgc gcggcactgt acctcaacgg ctgtcttcat cggcgcgcat
      481 cagtcgattg gtttgaaagc gctgctgctc atgggcaccg aggagcagaa gcggcagtac
      541 ctgccggcgc tggccagcgg tgagaagatc gccgcgtttt gtttgagcga gccgcaggtc
      601 ggctccgatg ccgccaatgt gcagacgacg gcgcgcctca gcgacgacgg ctcgcattgg
      661 atcctcaacg gcgaaaagaa gtacgcgacg aacgccgccc tggcggggtt catgacggtg
      721 atggccaaga cgccggtcac cgacgaacgc ggcaagacgc gcgacaaggt caccgcgttc
      781 atcgtcacgc cggacttgcc ggggttcgaa gtcgtgaaga gcaaccgcag caagtgcggc
      841 atccgcgggt cctggcaggc ggtgctgcgc ttcaccgaca tgcgcgtgcc aagcgaccgc
      901 atccttggcg ggcttggcaa aggtttaaaa gtcgcgctca gcgtcttgaa ctacggccgc
      961 tgcacgatga gcgccggctg cgtcggcggc gcgaagctgg cgctggagat gtgcatcgat
     1021 cgcgcgcgga cccgcaaaca atttggacgc gcgatcggcg aatttcacct ggtgaaggag
     1081 aagctcgcgc ggatggccga gagcgttttc gcgatggacg ccatgaccta tctggccgcg
     1141 gggctggtcg atcgacacgt tgacgacctg atgctggaga cggcgatctg caagctgttc
     1201 tgctccgaag cggcctggca agtgatcgac gacgccgtgc aactctgggg cggcgaaggc
     1261 tacatgcgtg agacggggct ggagcgcatg ctgcgcgacg cccgcatcaa ccgcatcgtc
     1321 gaaggcacca gcgaagtgat gaccgcgttc atctcgctgg tcggcatgaa aggcgtcggc
     1381 gaagacctgg agcaggtcat gcggctggcg aaacatcccg tcggcaactt cggacgttta
     1441 gccaccttcg cccgcggcga gtggagcgat gtcgtcatgg gccattcgct cgaagggctg
     1501 catccgaagc tgtcgaatga aggtcgaacc ttcgcccggc tgaccaagct cctggcccgc
     1561 gacgtcgtcc gcctgctcgg caagcatcgc gagcggatcc tggacatgga gctggtgcac
     1621 cagcgcgtga ccggcgccgc gatggaactg tacgcgatgg ccgcggtgat cagccggatg
     1681 cagatgctga tcgagcgcag cgagcgcgcg acagaaggca acggtcacga caagctgcgg
     1741 cgggatctga ccgtcggccg ctcgttttcc cggcacgcgg ctcagcgcgt cgagcggcat
     1801 ttagattcgc tgttcagcaa tcaggacgaa gatgtattgc aaaccgccaa cgcggtgctg
     1861 ggatgggaat gcgatccgag cgcggagtga gcacaggtgt catcctgaga gcaggcggta
     1921 ctccgccgga gtcgaacgat cttgccgaga cgtatgtgaa cgatcaattg cgcgatcggc
     1981 gcggcagtcg cttgcggttt cgcagacctg tgaccgcggc ttgtccgtct ccgaaaccgc
     2041 aagcgactgc cgagcgtttt tcggctggtg catgttcact cgcttctcgg caagatcctt
     2101 cgactgcgca cgagtacgtg ctccgctcag gatgacagac tcattggacg gcgcggcggc
     2161 gcctccgaca ttcaatgcgc cgtcggatac gcccgcttcg ccggtccgtt gtacagcgac
     2221 cgcgggcgga tcagccggtt gtgatcgtgc tgctcgatga tgtgcgcaca ccaccccgcc
     2281 acgcgggcgg cggcgaagac cggcacatcc agctccgggg ggatgcccat caggtgcagc
     2341 gccggcgccg cgtataaatc gaggttcgcg tacagcccct tctccttcgc catcacttct
     2401 tccagcttca cgcaaacatc caccagctgc tcgtgcccca gccgccggcc gagctgtcgc
     2461 gccaggtcgc gcatcgcggg cacgcgcgag tcgcccttct tatacacgcg atggccgaag
     2521 cccatgatct tgtccttgtg ggccaggtgc ttcttgaccc actcctcggc ataatccggc
     2581 gtgccgatct ccttcagcat cttcatcgcc tcttcgttcg ccccgccgtg cagcggccct
     2641 ttgagcgtgc ccagcccgct gacgacaccg gcgtagatgt ccgacaacgt ggacgccgtc
     2701 acgcgtgccg cgaacgtcga ggcgttgaat tcgtgctcgg cgtacagcgt gaagatcagg
     2761 ttcatcgcgc cgacccgcca cggctccggt tccttgccac tcatgccgaa cagaaatcgc
     2821 gccgcgaacg tcaggtgctc gccaggtgtg tacggttcct gcccgctgac gatgcgatgg
     2881 ctcgcggtga tgatcgtcga ggtccgcgcg atgatccgca tcgctttgcg caagttggcc
     2941 gcgtgcgaat tgtccggcag ctccggatcg aagctcgcca gcatcgacac gccggtgcgc
     3001 agcacgtcga tcgggtgcgc ccgcggcggc atcagcttca gcatcgaccg caccgccccc
     3061 ggcagcggcg cgtcggccgc cagttcttcg cgcgtctttt ctaattgcga tgtatccggc
     3121 agctcgccgt gtagcaacag gtagatgatt tcttcgaagc tggcgcgcac ggccaactcg
     3181 tggatatcat agccgcgata gagcaggccg gattcaccat cgaccgcgga gatggccgac
     3241 tcgccggcga tcacaccggc taggcccggg ctgtagcttg gcgcgctagc aggcgttgcg
     3301 ggattggtcg ttgtcatagc ttcacctccg tccgatgtgg cgatccgctg attggttcgc
     3361 gccgcgcgcc tctgccctct cgcaccgtac tccgggagtg ggctggggtg agggcgattt
     3421 gcgaacgacg aaatccgaaa tctgaatggc gaatgacgag ccgcgcgatg gcatgcggtt
     3481 cgtcattctt cttcggattt cattcgggga ttcgaattcc gtcattcggg tttcctcgag
     3541 aaaaatcacc ctcaccctaa ccctaaccct ctcccggcgt accgggagag gggaccagaa
     3601 tgcgcatgca atcgtgggca cgccgacaag agtctaccgc acaattcgat caccgtcgcg
     3661 gccaatcttc cgccgctgga tcgtagtcca gcaggtcgta caactcctgc cgcgtctgca
     3721 ttcgatcgag ccactgctgc tgtgtgccgc ggcgtttcag gtcattcagc agctctaacg
     3781 aagttctcat cgaggcgcga aacgccgact gcgggaagat gaccatacga taacccatcg
     3841 ccgccagttg ccgcacgctg agcagcggac tgcggccgaa ttccgtcatg ttcgccagca
     3901 gtggaacgtt aacggctttg gcgaattgcc ggaactccgc cggcgattgc aacgcttcgg
     3961 ggaagatcgc gtccgctcct gctttgacat aagcaatcgc tctttcgatc gcgccgtcga
     4021 tgccttcatc cgcccgcgca tcggtgcggg cgacgatcag gaaatcccca tcacgcc
//
LOCUS       DATIMH010000797         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1350715, whole genome shotgun sequence.
ACCESSION   DATIMH010000797 DATIMH010000000
VERSION     DATIMH010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350715"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1851
                     /locus_tag="VLI90_13885"
     CDS             <1..1851
                     /locus_tag="VLI90_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15345.1"
                     /translation="RATGAIWGYGGTFEQYQKTMVHPSRGIYRVYTHTDGSFVPPVDE
                     VQGHAPQATPRTEPGPEVLRQASVRVNKSLDDCLKSTKPLNQMQVQFLAKAYTVPGTP
                     AFHSAVAVERIVTSIDDLYRRWRSDPEALWQDKATWNPGWFGVGPAGDAVRRLHEQLE
                     PALNQNLDGNTSRRTGWSAMFQASRDWLRDNRRWLTNQAMFTDTNLYLCNRAVAAIDP
                     SHALPEDRALDYLYQSVGLVPWLGRDTPQGPEKLFGSAFYQVTSKGLTRERGYVGSYG
                     EGGVEGAIDIYNATRESDGAGDPKIKAQLIKMLRARGPFRYPMLDEENHPAMRLETTV
                     GWRDTHEPGDVTYAQRVGNDHSALEAAVMTMDPVVIGYVQQMFADNQFFAAVAHHLKD
                     TRLRGTIGLLDESAQYQLMSAQPATGHKLPMSWDQPDFVFSDEEDGVVAIKHGDEILY
                     ASLYWRAGYGVNSLARAHYLTPDYQQVAVVHEQAITPNIGHAYKRPDWINFGFGDGGT
                     HIRYPADVHQAYAGEELPIAPIPADVKTKPGEESHFAGRADFYTLRFGQYLIAMNTTA
                     DRSFDLPEPDGSAEADDLVAGKRVKLSKSMPIGPKSTAVFYIAHQEPRTK"
BASE COUNT          440 a          711 c          680 g          337 t
ORIGIN      
        1 cgcgccaccg gcgccatctg gggatacggc ggcacgttcg agcaatacca aaagacgatg
       61 gtgcacccgt cgcgcgggat ctatcgcgtc tatacacaca ccgacggctc gttcgtcccg
      121 ccggtcgatg aagtgcaagg ccacgcgccg caggcgacgc cgcggactga gcccgggccg
      181 gaagtgctgc ggcaggcgtc ggtccgcgtg aacaagtcgc tcgacgactg tttgaaatcg
      241 accaagccgc tcaaccagat gcaggtgcaa ttcctggcca aggcgtacac ggtgccaggc
      301 acgccggcat ttcactcggc ggtcgccgtc gagcgcatcg tcacgagcat cgatgatctg
      361 taccggcgct ggcggtcgga tccggaggcg ctgtggcagg acaaggcgac gtggaacccc
      421 ggttggttcg gcgtcggtcc cgccggggat gccgtgcgac gactgcacga gcaactcgaa
      481 cccgcgctga atcagaacct ggatggcaac acgtcgcgcc gaaccgggtg gtcggcgatg
      541 ttccaggcca gtcgcgattg gctgcgcgac aatcgccggt ggttgaccaa ccaggcgatg
      601 ttcaccgaca caaacctcta cctgtgcaat cgtgccgtgg cggcgatcga tccatcgcac
      661 gcgctgccgg aggatcgcgc gctcgattat ctgtatcaaa gcgtcgggct ggtgccgtgg
      721 ctgggccgcg acacgccgca gggtccggag aaactgttcg gcagcgcgtt ctaccaggtc
      781 acgagcaaag gcctgacgcg cgagcgcggc tacgtcggaa gctacggcga aggcggcgtt
      841 gaaggcgcga tcgacatcta caacgccacc cgcgaatcag acggcgcggg cgatccgaag
      901 atcaaagcgc agctgatcaa gatgctgcgc gcccgcgggc cgtttcgtta cccgatgctg
      961 gacgaggaga accacccggc gatgcggctg gagacaaccg tcggttggcg cgacacgcac
     1021 gaacccggcg acgtcacgta tgcccagcgc gtcggcaacg atcattccgc gctggaagcg
     1081 gccgtcatga cgatggaccc ggtcgtcatc ggatacgtcc agcagatgtt cgccgacaac
     1141 cagttcttcg ccgccgtcgc ccatcatctg aaagacacgc ggctgcgcgg cacgatcggc
     1201 ctgctcgacg aaagcgctca ataccaactc atgagcgcgc agccggcaac gggacacaag
     1261 ctgccaatga gctgggacca gcccgacttc gtcttcagcg atgaagagga cggcgtcgtc
     1321 gccatcaagc acggcgacga gatcctgtac gcctcgctgt attggcgcgc tggttacggc
     1381 gtgaactcac tcgcgcgcgc gcactatctc acgccggact atcagcaggt cgcggttgtc
     1441 cacgagcaag ccatcacgcc caacataggt cacgcgtaca agcggccgga ctggatcaac
     1501 ttcggcttcg gcgacggcgg cacgcacatc cgctaccccg ccgatgtgca tcaagcctac
     1561 gccggcgaag agctgccgat cgcccccatc cccgccgacg tgaaaaccaa acccggcgaa
     1621 gaaagccact tcgcggggcg cgcggacttc tacacgctgc gcttcggcca atacctgatc
     1681 gcaatgaaca cgaccgccga tcggtcgttc gatctccccg agccggatgg aagcgccgag
     1741 gccgacgatc tggtggccgg gaagcgcgtg aagctatcga agtccatgcc gatcggtccg
     1801 aagtccacgg cggttttcta catcgcgcat caagagccgc gcacgaagta agtggaaaag
     1861 gggcgctggt gcctgcttca cttgatcagc ggaaccagct catcgtcaac caatgcgaaa
     1921 ggcactgccg ccgccgccga gcgcggttgc gtccagaagc gcagggtctc cacgcccacc
     1981 gcgagcaaca tggagtctta agtaagcgct accaagtttt ttttcgaacg ggccaggcac
     2041 gatgaacttc gcaacgaccg acgttgaaga aacgcgtccc acgccgttcg gaggtgctcc
     2101 gacgccccgg atgcttctcg ctcgcggcag aatctcgctt cgtcggagta cctccgaacc
     2161 cggcgtgg
//
LOCUS       DATIMH010000798         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5105084, whole genome shotgun sequence.
ACCESSION   DATIMH010000798 DATIMH010000000
VERSION     DATIMH010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5105084"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1524)
                     /locus_tag="VLI90_13890"
     CDS             complement(<1..>1524)
                     /locus_tag="VLI90_13890"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSV15346.1"
                     /translation="DISDALNFGDKENVLAVRVDNRTDYKEKSSGVGFEWSSKDFNPN
                     YGGINRRVWLHVAGKIYQTLPLFYGLQTTGVYVYPSNISVADKTADVNVEAQVQNGSG
                     DQASITLSTVVVDADGQVHATFDGETIDMVAGEKTVHKATGKLSGAKLWSIESPYLYD
                     VYTILKVNDKVVDVNKVRTGFRKTDFRGGAGTGGVYINDKFVWLTGYAQRATDEWAGL
                     GQAYPAWMHDLTAQLLRASNGNYMRWMHISPQPVDVEAYDRAGIVEVCPAGDKEKDAT
                     GRQWEQRMEVMRASMIYMRNHPSILFWEAGNNGISADHMKQMVDLQQELDPSGGRVIG
                     CRTLNDPNTTPIAGYFGVMIGQDPRTDALKSTTQMFRAYSAERRDRAPIIETEDFRDE
                     AARRFWDDYSPPHFGFKPGPNDTYKWNSETFCLAAANRYWAYWSNRISNKDPAHAKWS
                     GYASIYFTDSNADGRQDSSEVARVSGKVDAVRLPKEAFYTYRVMQSEKPDIHIIGHWT
                     "
BASE COUNT          242 a          499 c          450 g          333 t
ORIGIN      
        1 gtccagtggc cgatgatgtg gatgtccggt ttttcgctct gcatcacgcg ataggtatag
       61 aacgcttcct tcggcaggcg caccgcgtcc accttcccgc tcacgcgggc gacctcgctc
      121 gagtcctgcc ggccgtcggc gttggaatcg gtgaagtaga tcgacgcgta gccggaccat
      181 ttcgcgtggg ccggatcttt gttcgagatg cgattggacc agtacgccca gtaccgattg
      241 gccgccgcca ggcagaacgt ttcgctgttc cacttgtacg tgtcgttggg ccccggtttg
      301 aacccgaagt gcggcgggga ataatcatcc caaaaccgcc gggcggcttc gtcgcggaaa
      361 tcttcggtct cgatgatcgg cgcgcgatcg cgccgctcag cgctgtacgc gcggaacatc
      421 tgcgtggttg acttgagcgc atcggtccgc gggtcctggc cgatcatcac cccgaaatat
      481 ccagcgatcg gcgtcgtgtt cgggtcgttc aacgtccggc aaccgatcac gcgcccgccg
      541 ctcggatcca gctcctgctg aaggtcaacc atctgcttca tgtgatcggc gctgatgccg
      601 ttgttgccgg cttcccagaa caggatgctc gggtgattgc gcatgtaaat catcgacgcg
      661 cgcatcacct ccatgcgctg ctcccactgc cggccggtgg cgtccttctc cttgtcaccc
      721 gcggggcaga cttcgacgat gcccgcgcga tcgtacgcct cgacatcgac cggctggggg
      781 ctgatgtgca tccagcgcat gtagttcccg ttgctggcgc gaagcagctg cgcggtgagg
      841 tcgtgcatcc acgccggata cgcctggcca agccccgccc attcatccgt cgcgcgctgg
      901 gcatagccgg tcagccagac gaacttatcg ttgatgtaca ccccacccgt gccggccccg
      961 ccacggaagt cggtcttgcg aaacccggtg cggaccttgt tcacgtccac gactttgtcg
     1021 ttcaccttca ggatcgtgta gacgtcgtac aggtacggcg actcgatcga ccacagcttc
     1081 gcgccgctca atttccccgt cgctttgtgg accgtctttt cgccggcgac catgtcgatg
     1141 gtctcgccgt cgaacgtcgc gtgcacttgt ccatcggcat caacgacgac cgtcgacagc
     1201 gtgatcgacg cctggtcgcc tgatccgttt tgcacctgcg cttcgacgtt cacgtcggcg
     1261 gttttgtcgg ccaccgaaat gttgctcgga tagacgtaca cgccggtggt ttgcaggccg
     1321 tagaacagcg gcagcgtttg gtaaatcttc cccgcgacgt gcagccacac ccggcggttg
     1381 atgccgccgt agttggggtt gaaatctttg ctcgaccact cgaacccgac gccgctcgac
     1441 ttctccttgt aatccgtgcg attgtccacg cgcaccgcca gcacgttttc cttgtcgccg
     1501 aagttcaggg cgtccgagat gtcg
//
LOCUS       DATIMH010000799         8055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1351074, whole genome shotgun sequence.
ACCESSION   DATIMH010000799 DATIMH010000000
VERSION     DATIMH010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351074"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..367
                     /locus_tag="VLI90_13895"
     CDS             98..367
                     /locus_tag="VLI90_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HSV15347.1"
                     /translation="MVPSQKDWPEWWSWELECSNPHLAKRMIDRGFNEVELRDMLQRA
                     NGYRPDPVSGRWVITTTHAGNPWEVVVEPQPAAKLLVVVTASEEG"
     gene            372..638
                     /locus_tag="VLI90_13900"
     CDS             372..638
                     /locus_tag="VLI90_13900"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HSV15348.1"
                     /translation="MRTPYLEVTYRQGKPFAAYLYLDRRPGDRAATTKRDGDLVIDYA
                     PDGRPIGIEFMQVAAVDLSAVNRVLERAHETSLSANDLSPLSAA"
     gene            complement(1345..6201)
                     /locus_tag="VLI90_13905"
     CDS             complement(1345..6201)
                     /locus_tag="VLI90_13905"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HSV15349.1"
                     /translation="MKITRQFTKAGQDVFSTVDYEKRTSRISNPDGSVVFQMNDAEIP
                     KSWSQLATDIMVSKYFRKAGVPQTDENGAPVRDANGNIVTGPEKSAKQVINRLAGCWR
                     YWGEKHGYFDTADDAQAFYDELSHMLLHQMCAPNSPQWFNTGLNWAYGITGPAQGHFY
                     CDPRSGELLKSKDAYSHPQPHACFIQSVSDDLVNAGGIMDLWTREARLFKYGSGTGSN
                     FSKLRGENEPLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVCLDLDHPDVEQ
                     FINWKVREELKVACLAEGIKHLPGDQQELANQLGLKLDYDFNGEAYFTVSGQNSNNSV
                     RIPNRFFKAVEDDGEWTLTNRTNGKVAKKIKARDLWQQISYAAWRCADPGVQYDDTIN
                     QWHTCPRSGRINASNPCVTGDTRVLTPGGIWRRIDQMIHLPARIITNLERQEIHVTDG
                     SFPTGTKDVYELRTTGGYSVKLTGDHKIWTRNRGWVEAKDLTTKDEVKLPSKPAAVQE
                     IGEPQDAKFFQLLGLFLSNVNNSNHSLRLDKCLANVSTAEEFAQYVTDTWGDRAYADD
                     YVNHLMMDGAAPNAVGASESEESTATSGEGSAPTLTAAVTHRRLLSRMTAFLRTEHGA
                     TRFSDEAFTAGLAAQKHVLRGLFTADAAISNTLELHHNSLGLLQDVQLILLGFGVQSS
                     IANDSTDGGAVARGALPADRQVNTTPLGLRDDQSISSSRGDANHRRHGLRIDPGSLRS
                     FGKHVGLLPGRKLQQLATLISHTLARPDGAANWDTIASFAHLGRQQVFDLTEPHTHSF
                     IAGGITVHNCSEYMFIDNTACNLASLNVLTFFDAETRSFDIEAFKHGTRIWTIVLEIS
                     VLMASFPSEDIAQLSYKFRTLGLGYANLGAMLMQAGIPYDSDKGRGICAALTAILTGE
                     SYATSAEMARELGTFPGYAENKLDMLRVIRNHRRAAWGVGADPSAANTHASPSAGFGT
                     LGDYETLDIHPVPIDPAQFADTDPLASRRLLSAAQECWDRALYLGEMHGYRNAQTTVI
                     APTGTIGLLMDCDTTGVEPDFALVKFKKLAGGGYFKIANQSLRPALVNLGYTPEQIHD
                     ILRYVMGTLSLHDAPYINWENLKTHGFTEAELHKIEDILPSQFEISFAFTGWSLGADI
                     FKRLDVPEAEWQSPNFNLLKKLGYTKKQIDEANDVICGVGTVEGAPHLRPEHYPVFDC
                     ASKCGKHGTRIIPAEGHIRMMAAAQPFISGAISKTINLPNEATVEDIKQSYELSWKLG
                     LKANALYRDGSKLSQPLNVKSDEEVEKATDEEDPEAVEAAREEIAQEATTGVMTGVVA
                     SATMSPAAHVTSDNLEPTHTKVIEKIIERIVERPLRRRLPDTRHAITHKFDVAGHEGY
                     ITAGLYEDGQPGEVFITMAKEGSTIGGLMDAIATLVSVSLQYGVPVESLVRKFEHVRF
                     EPSGMTRNQDIPMAKSLVDYIFRWLAMEFIPGYRAANAPQRAKKPAAASAPEAATLPP
                     SPGTADRRSPGEGRGEGGSPPANAKSSKKSGNGHGPRMDKDTAHPFAYSDEELSEAHS
                     TKYQANEAHNAPAGANLRLAIVADPLSQQGTEMQADAPACDVCGSITVRSGTCYKCLN
                     CGNSMGCS"
     gene            complement(6342..6842)
                     /locus_tag="VLI90_13910"
     CDS             complement(6342..6842)
                     /locus_tag="VLI90_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15350.1"
                     /translation="MASARFYVLSSVAVMGVAAVLGGCTDQNKGSSYPPPNTPSSSYQ
                     SSSYQSSSTVTPAQQNANDSSAQTAGMTTPPPVRVPAGARMVSAGPYPPPSFNASEGG
                     TLYIWDDDENKIALVTSVGNSDVNRNVNIADMPNVSNALNNKHRFRVYYVPTSAATTL
                     PSSLGQ"
     gene            complement(6873..7433)
                     /gene="dcd"
                     /locus_tag="VLI90_13915"
     CDS             complement(6873..7433)
                     /gene="dcd"
                     /locus_tag="VLI90_13915"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HSV15351.1"
                     /translation="MSILCDSQIRELIGIEPFEENIKRPGKVSYGVSSYGYDLRVGPV
                     FKIFTNVNSEIIDPKRFSDRSFVTVDTEETGKDHVLIPPNSFALCETVETVSMPRDYL
                     AICVGKSTYARCGIIVNVTPIEPEWRGKITLEISNTTPLPAKIYANEGIAQLIFLKGE
                     RVCAVSYADKSGKYQDQPGLTLPRVD"
BASE COUNT         1448 a         2354 c         2509 g         1744 t
ORIGIN      
        1 cgccgcggca gttactgggc cacccaattg agctgacgcg acggcgttcc cgttttgtcc
       61 ccggttggct gttttccttt gcgcgggcag gttcggcgtg gtaccatcac agaaggattg
      121 gccggagtgg tggtcgtggg agctggaatg ctccaatccg cacttggcca agcgaatgat
      181 cgaccgcggt ttcaacgaag tcgaactccg cgacatgctg cagcgcgcca acggctatcg
      241 tcccgatccc gtgtccggtc gctgggtcat caccaccact cacgccggca acccgtggga
      301 agtcgtggtt gaacctcaac ccgctgcaaa actgctggtc gtcgttacgg catctgaaga
      361 aggatgaagc aatgcgcacg ccctatctag aagtcaccta ccggcaaggc aagccgttcg
      421 ccgcgtacct gtatctggac cggcgtccgg gcgaccgcgc cgcgacgacg aaacgcgatg
      481 gcgatctcgt gatcgattat gcgcccgacg gccggccgat cggcatcgaa ttcatgcaag
      541 ttgccgctgt cgacctctca gcggtcaacc gcgtactcga acgagcgcac gagacgtcgc
      601 tctccgccaa cgatctgtcc ccgctgtccg ccgcgtgaca accgcgttcg ggaggccaga
      661 acatggccca cgggtacggc gagctgctca agatgggcca cgtcgcccga aaaaagccga
      721 acgcacggcg acgcaccgcg ttgcaaaaca cctctcccct tgcctctact gtatgccccg
      781 aagttacatc ctggccacat ttcttgaagg ctcaaagtaa gtccagatca attccggtga
      841 taagccgctg tctcacatgg tttgcacgat gtcctcttcc ttcagcaacg tgttttggtt
      901 cgggaagccc aaactacgaa acacgcctct gatgcgattg acttgccggg tgatgggcca
      961 cccggcagag atcgagtcga tcggcaatct cgcgggcatt gtaaccatct aagaaatagc
     1021 accggtaagt ataccgttgc ttggcggtca gcgcagctag agcggcgttt tatgctcctg
     1081 gaaaattcat ctcgatgttg ccaacccgac atctttccaa tgctcggtcg cctgattgca
     1141 catacgcctt cggcactctc ccgagacagt ggttaatgta acggcagacg gtcctccgtg
     1201 agacgttcag ttcggttgcg atctcgctat aggtgagccc gtccgataag tacatcgtga
     1261 ccgcacgtcg tcttagtcgt tcaacttgaa gatgcgtcct cttcgccata cgaattcaaa
     1321 tgtggctaag cgtcaaccaa gccgttaact acaccccatc gaattcccgc agttcaagca
     1381 cttgtaacac gtgccgctgc ggacggtgat cgagccgcag acgtcgcagg cgggggcatc
     1441 cgcctgcatc tcggtgcctt gttggcttaa ggggtcggcg acgatggcca atcgaagatt
     1501 ggcgccggcg ggggcgttgt gggcttcgtt cgcttgatac ttggtgctgt gagcttcgct
     1561 gagttcttcg tcgctgtagg cgaaggggtg ggcggtgtct ttgtccatgc gggggccgtg
     1621 tccattaccc gacttcttgg aagacttggc gttcgccggt ggcgaaccac cctcaccccg
     1681 gccctctccc ggcgacctgc ggtcagcagt accgggcgag gggggaagag tagcggcctc
     1741 gggcgcgctg gcggcggcag gcttcttcgc gcgctgcggc gcgttggcgg cgcggtagcc
     1801 ggggatgaat tccatggcga gccagcggaa gatgtaatcg accaggctct tggccatggg
     1861 gatgtcctgg ttccgggtca tgccgctggg ttcgaatctg acgtgttcga attttctcac
     1921 gagtgattcg acagggactc cgtactgcaa gctgacggag accagggtgg cgatggcgtc
     1981 catcaggccg ccgatggtgc tgccttcttt ggccatggtg atgaagactt cgccgggctg
     2041 gccgtcttcg tataggccgg cggtgatgta gccttcgtgg ccggcgacgt cgaacttgtg
     2101 ggtgatggcg tgtctggtat ctggaagacg acggcgcagg gggcgctcga cgattcgttc
     2161 gatgatcttt tcgatcacct tggtgtgggt gggttcgagg ttgtcgctgg tgacgtgcgc
     2221 ggcgggggac atggtcgctg aagcgaccac gccggtcatt acgccggtgg tggcttcttg
     2281 cgcaatttct tcgcgcgcgg cttctaccgc ttcggggtct tcttcgtctg tcgccttctc
     2341 gacctcttcg tcgctcttta cgttcagcgg ttgactgagt ttgctaccat cgcgatagag
     2401 cgcgttggcc ttcaagccaa gtttccagct tagttcgtag ctttgcttga tgtcttcgac
     2461 ggtcgcttcg ttggggaggt tgatggtctt gctgatcgcg ccgctgatga acggttgggc
     2521 ggcggccatc atgcggatgt ggccttcggc ggggatgatg cgggtgccgt gcttgccgca
     2581 tttactggcg cagtcgaaga cggggtagtg ctcggggcgc aggtgcgggg cgccttcgac
     2641 ggtgccgacg ccgcagatga cgtcgttcgc ttcgtcgatt tgcttcttgg tgtagccgag
     2701 ttttttgagc aggttgaagt tgggcgactg ccattcggct tcggggacgt cgaggcgttt
     2761 gaagatgtcg gcgccgagcg accagccggt gaaggcgaag gaaatttcga attggctggg
     2821 caggatgtct tcgatcttgt gcagttcggc ttcggtgaag ccgtgggttt tgagattttc
     2881 ccagttgatg tagggggcgt catgaaggct gagcgtgccc atcacgtagc ggaggatgtc
     2941 gtggatctgc tcgggcgtgt aaccgaggtt caccagcgcg ggacggaggg actggttggc
     3001 gattttgaag tacccaccgc cggcgagctt cttgaatttg acgagggcga agtcgggttc
     3061 gacgccggtg gtgtcgcagt ccatcaaaag accgatcgtt ccggttggtg cgatgacggt
     3121 ggtctgggcg ttgcggtagc cgtgcatctc gccgaggtag agggcgcggt cccagcattc
     3181 ctgtgcggca ctgaggaggc gtcgcgaggc gaggggatcg gtgtcggcga actgggcggg
     3241 atcgatgggg acggggtgga tgtcgagggt ttcgtagtcg ccgagggttc caaagcctgc
     3301 ggatggcgat gcgtgggtgt tcgctgcgct ggggtcggcg ccgacgcccc aggcggcacg
     3361 gcggtggttg cggatgacgc ggagcatgtc gagcttgttt tcggcgtatc cggggaacgt
     3421 gccgagttcc cgggccatct ccgcgctggt ggcgtaactt tcgccggtga gaattgcggt
     3481 aagggcagcg cagatgccgc ggcccttgtc gctgtcgtac ggaatgccgg cttgcatgag
     3541 catcgcaccc aggttggcgt agccaagtcc cagcgtgcgg aacttgtaac taagctgcgc
     3601 gatgtcttcg ctggggaagc tggccatcag aacggagatc tcgagcacga tcgtccagat
     3661 gcgcgtgccg tgcttgaacg cttcgatgtc gaagctgcgc gtctcggcgt cgaagaaggt
     3721 caggacattc agcgaagcca aattgcacgc cgtgttgtcg atgaacatgt attcgctgca
     3781 gttgtgcacc gtgatgccgc cggcgatgaa gctgtgggtg tgcggctcgg tcaggtcgaa
     3841 gacctgctgg cggcccagat gcgcgaagct ggcgatagtg tcccagttcg ccgcgccgtc
     3901 ggggcgggcg agggtgtggg agatcagtgt ggccaattgt tgcagctttc ggcccgggag
     3961 aaggccgacg tgctttccaa aagagcggag gctgcccgga tcgatacgca agccgtggcg
     4021 tctgtgattt gcgtcgccgc gcgatgacga gatggactgg tcgtcgcgca ggccaagtgg
     4081 ggttgtgttc acttgccgat cggcgggcag cgctccgcgt gcgacggctc cgccgtcggt
     4141 tgagtcgttg gcgattgacg attgaacgcc gaagcccagc aggatcagtt ggacgtcctg
     4201 caagaggccc aggctgttgt gatgaagttc gagcgtgttg ctgatcgcag catcggcggt
     4261 gaagagaccg cgcaggacgt gtttctgcgc cgcgagcccc gcggtgaagg cttcatcact
     4321 aaagcgcgtc gcgccatgct cggtgcgcag gaacgccgtc atccggctca gcaaccgccg
     4381 atgagtgacg gcggcggtga gggtaggagc cgaaccctca ccgctcgtcg ccgtcgactc
     4441 ttcagattca gaagcgccca ccgcgttcgg cgcagcgccg tccatcatca agtggttgac
     4501 ataatcatcc gcgtacgcgc ggtcgcccca ggtgtcggtg acgtactgcg caaactcctc
     4561 cgccgtgctc acgttcgcca ggcacttgtc caagcgcagc gagtggttgc tattgttcac
     4621 gttcgacagg aacaatccga gcagttggaa gaacttcgca tcctgcggct cgccgatttc
     4681 ctgcaccgcc gcgggcttgc tcggcagctt gacctcgtcc ttcgtcgtca ggtctttcgc
     4741 ctcaacccag ccgcggttgc gcgtccaaat tttgtgatcg ccggtcagct tcacgctgta
     4801 tccgccggtg gtgcgcagct cgtacacgtc tttcgttccg gttgggaacg aaccgtcggt
     4861 gacgtggatt tcctgccgtt ctaaattcgt gatgatgcgc gccggcagat ggatcatctg
     4921 atcgattcgc cgccatatcc cgccgggggt cagcacgcgg gtgtcgccgg tcacgcacgg
     4981 gttgctcgcg ttgatgcgcc cactgcgcgg acaggtgtgc cattgattga tcgtgtcgtc
     5041 gtactgcacg ccgggatcag cgcagcgcca ggcggcgtat gaaatctgct gccacagatc
     5101 gcgcgccttg atcttcttgg cgaccttccc gttggtgcga ttggtcagcg tccactcgcc
     5161 gtcgtcttcc accgctttga aaaagcgatt gggaatgcgc accgagttgt tcgaattctg
     5221 tccagagacg gtgaagtacg cctcgccatt gaaatcgtaa tccagcttca gccccagctg
     5281 gttggccagt tcctgctgat ctcccggcag atgcttgatc ccttccgcca ggcacgccac
     5341 tttcagttcc tcgcgcactt tccagttgat gaactgctcg acatccgggt gatccagatc
     5401 gaggcaaacc atcttcgccg cccgacgggt ggtgccgccg gacttgatcg cgcccgccgc
     5461 ccgatcgcca atcttcaaga acgacatcag gcccgacgat tttccgccgc ccgaaagcgg
     5521 ctcattttca ccgcgcagtt tcgagaagtt cgaacccgtg ccagagccgt acttgaacag
     5581 ccgcgcttcg cgcgtccaca ggtccatgat cccgccggcg ttcaccaggt cgtccgacac
     5641 cgattgaatg aaacacgcat gcggctgcgg atgtgaatac gcgtccttgc tcttaagcaa
     5701 ctcgccgctt ctgggatcgc aataaaagtg gccctgagcc ggcccggtga tgccatacgc
     5761 ccagttcaaa ccggtgttga accactgcgg gctgttgggc gcgcacattt ggtgcagcag
     5821 catgtgcgac agctcatcgt aaaacgcctg cgcgtcgtcg gccgtgtcga aatatccgtg
     5881 cttctcaccc caataccgcc agcaaccggc caggcgattg atcacctgct tcgccgattt
     5941 ttcgggcccg gtgacgatgt tcccattcgc gtcacgaacc ggcgcgccgt tctcgtccgt
     6001 ctgcggcacc cccgccttgc ggaaatactt gctcaccatg atgtcggtgg ccagctggct
     6061 ccaactcttg gggatctccg cgtcgttcat ctgaaaaacg accgaaccat ctggattaga
     6121 aatgcggctc gtccgcttct cgtaatcgac cgtcgaaaag acgtcctggc ccgctttggt
     6181 gaattggcgc gtgatcttca tgagtaggtt ccttccctgt acgaatctaa cattatccta
     6241 acatcgctag gcgatcggac aatggccctc ccgcacgcgc gacagccccg tcgcgcgtgt
     6301 ggtgactggt ttaattgtga cacgaatgtc gcggggtcag cttattgacc cagcgacgac
     6361 ggcagcgtgg tcgcggccga cgtgggaacg tagtagacgc ggaatcggtg tttattgttt
     6421 agcgcattgg agacgtttgg catgtccgcg atgttcacgt tgcgattcac atcgctgttg
     6481 cccacgctcg tcaccagcgc gattttgttc tcgtcgtcat cccagatgta cagcgtcccg
     6541 ccttcggacg cgttgaacga cggcggcgga tacgggcctg cggaaaccat ccgcgcgccg
     6601 gctggcacgc gaactggcgg tggcgtcgtc attccggccg tctgcgcgct ggagtcattc
     6661 gcattctgct gcgccggcgt taccgtcgac gacgactgat acgacgacga ctgataagaa
     6721 gacgacggcg tgttcggcgg aggatacgag ctgcccttgt tctgatcggt gcaaccgccc
     6781 aacacggcgg cgacgcccat gacggccacg gatgaaagaa cgtaaaaacg tgcagaagcc
     6841 atggagggcc tcctttcaca aattgaaggg acttagtcga cccgcgggag cgtcagcccc
     6901 ggctggtcct ggtacttgcc gctcttgtcc gcgtaactca ccgcgcacac ccgttcgccc
     6961 ttcaaaaaaa tcaactgcgc gatcccctcg ttggcgtaaa tcttcgccgg caggggggtc
     7021 gtgttcgaaa tctccagcgt gatcttccca cgccattccg gttcaattgg cgtcacgttc
     7081 acgatgatcc cgcaccgcgc gtacgtgctc ttgcccacac agatcgccag gtaatcgcgc
     7141 ggcatcgaca ccgtctccac cgtctcgcac agcgcaaagc tgttcggcgg aatcaacacg
     7201 tgatccttcc ccgtctcctc ggtgtccacc gtcacgaatg aacgatcgct gaagcgcttc
     7261 ggatcgatga tctcagaatt aacgttggtg aagatcttga acaccggccc gacccgcagg
     7321 tcatagccgt agctcgacac cccgtagctg actttgcccg gccgcttgat gttctcttcg
     7381 aacggctcaa tgccgatcaa ttcccggatc tggctgtcgc aaaggatgct catgagaagt
     7441 gtgcctgccc aaatcaaccg gtgcagaacc atccgcgacc gaggactccg tcctccatcg
     7501 cgcgtgaacg accaccggca cgcgactttc ttaggacgcc atcttcccta acagcgccac
     7561 gcaactgtaa cggacaaaac gaacggaccg cccaagcgcc gttgcgacgc ctcgacttgc
     7621 cgagagacca actttgcaat ctgagtgccg aattccgtca gccaaccagc ggcggcaggc
     7681 tttcgaccct cgcccattcg ttccccacgg aacgaggcgg actatacact acatctggta
     7741 gaggtcaatc ggaactttac taaatctagc gtagttagcc gacactgcat tgctagcagt
     7801 tgatattgcc gcggttagga tagtgtgaaa tcgatcgagc gaaatgtttg atcgcgcgcg
     7861 gggccaagtt atccaccgct cgtcaccgcg gaggtggtgg ataagggcgc aggaatgcgg
     7921 cggcgcgaga aatttttcag tcgttcctgg aggcgttctg cgccgcacga gcaggtacga
     7981 cattgtcgca tgtgtcggaa tgcggaatga tgaatgagct atgaccaaaa gctgtggatg
     8041 acgacgcggc aataa
//
LOCUS       DATIMH010000800         1650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2211402, whole genome shotgun sequence.
ACCESSION   DATIMH010000800 DATIMH010000000
VERSION     DATIMH010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211402"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1236
                     /gene="sppA"
                     /locus_tag="VLI90_13920"
     CDS             109..1236
                     /gene="sppA"
                     /locus_tag="VLI90_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSV15352.1"
                     /translation="MSTQPPPGQGPIDPRGAFAPPPPPAGYMPPPPPPGWYPPMPPAP
                     RQRGFARAIFTTLATTLFGISIALNIYLILFGALASARSSSRENVVVAGDETQKVAVV
                     PIVNSMMLEKQAEQFDRAMSAVEKDGNVKALVLKIDTPGGAVTSADEMYHRIIVFKQR
                     KPGIPVIVTMAGYATSGGYYAACASDYIFAQPTTITGNIGVLMPNYNMSKLADKWGIE
                     DASLHSTGADYKTAGSWLRPQTPEERAYLLDLIDSAFKQFKSVVSTGRKDRLTQNIDA
                     IANGKAYTADEAKQLGLVDQIGYEADAYQYAAPKAGLTNMQVVRFDEPASLLDMFTAS
                     STLPQPKASASGGVQINGIQVDASKIDELLTPRLLYLWRGE"
     gene            1390..>1650
                     /locus_tag="VLI90_13925"
     CDS             1390..>1650
                     /locus_tag="VLI90_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15353.1"
                     /translation="MMRSSIGVVLVLVTLLVSPAFAATPDQIEAAIQKAKAFVYSQQK
                     EGIWEKRADPPPDSMRKEKGFNYKIDGGQWGGLTALATYALVA"
BASE COUNT          332 a          532 c          516 g          270 t
ORIGIN      
        1 agcgccgcag aatctcgctt cgtcggagta cctccgaacc cggcgtgggg gcagactttt
       61 ccctttcccc gcgccgaccg gtcatctatc attaacgctg gaaacacgat gagcacgcaa
      121 ccgccaccgg gacagggacc aatcgatccg cgaggggcgt tcgcgccccc gccaccgccg
      181 gcggggtaca tgccgccgcc cccgccgccg gggtggtatc caccgatgcc gcccgcgccg
      241 cgtcagcgcg ggtttgcccg cgcgatcttc accacgctcg cgaccaccct cttcggcatc
      301 tcgatcgcgc tcaacattta tctgatcctc tttggcgcgc tggccagcgc gcggtcgtcc
      361 tcgcgcgaga acgtggtggt cgccggcgat gagacgcaga aggtcgcggt cgtgccgatc
      421 gtcaattcga tgatgctcga aaagcaagcc gagcagttcg accgcgcgat gtcggcggtt
      481 gagaaggacg gcaacgtgaa agcgctggtg ctgaagatcg acacgcccgg cggggcggtc
      541 acttcggcgg atgagatgta tcaccgcatc atcgtcttca agcagcgcaa gccggggatt
      601 ccggtcatcg tgacgatggc cggttacgcc accagcggcg ggtattacgc cgcgtgcgcg
      661 agtgactaca tcttcgcgca gccgacgacg atcaccggca acatcggcgt gctgatgccg
      721 aactacaaca tgagcaagct ggccgacaag tggggcatcg aagacgcgtc gctgcactcg
      781 accggcgccg actacaagac ggccggttcc tggttgcgcc cacaaacacc cgaagagcgg
      841 gcatacctgc tcgacctgat cgactcggcg ttcaagcagt tcaagagcgt cgtctccacc
      901 ggtcgaaaag accggctgac gcagaacatc gacgcgatcg ccaacggcaa ggcgtacacc
      961 gccgacgagg ccaagcaact gggcctcgtc gaccagatcg gttacgaagc cgacgcgtac
     1021 caatacgcgg caccgaaagc cgggctgacg aatatgcaag tggtaaggtt cgacgagccg
     1081 gcgtcgctgc tggatatgtt taccgccagt tcgacgctgc cacagcccaa ggccagcgcc
     1141 agcggcggcg tgcagatcaa cggcatccag gtggacgcca gcaagatcga cgaactgctc
     1201 acgccgcggc tgctgtacct gtggcgcggc gagtgacctt tctggtcccc tggcccatgg
     1261 gcgcgtgcga gagggcgagt gcgattgcat gctcaattga caattgacaa ttttcaattg
     1321 tcaattgaat tcaatcgccc taccccagcc ctctcccggc gtaccgggcg agggggaaag
     1381 actgcgacga tgatgcgtag ctcgatcggc gtcgtgctcg tgttggtgac gctgctcgtg
     1441 tctcccgcct ttgccgcaac gccggatcaa atcgaagccg ccatccagaa ggccaaagcc
     1501 ttcgtctaca gccaacagaa ggaaggcatc tgggagaagc gcgcggatcc cccgccggat
     1561 tcgatgcgca aggagaaggg tttcaactac aagatcgacg gcgggcaatg gggcgggctc
     1621 acggcgctgg caacgtacgc gctggtcgcc
//
LOCUS       DATIMH010000801         6220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5105825, whole genome shotgun sequence.
ACCESSION   DATIMH010000801 DATIMH010000000
VERSION     DATIMH010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5105825"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..951
                     /locus_tag="VLI90_13930"
     CDS             178..951
                     /locus_tag="VLI90_13930"
                     /inference="COORDINATES: protein motif:HMM:NF004850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HSV15354.1"
                     /translation="MAVKPRSAHLHAGPGFRVKVNFQRPPQELIKGFADFPTPDISDL
                     LNRLYAVDREIRVLTNPDHRVCGAACTVKVFPGDNLMVHKALDVLEPGDVVIVDAGGS
                     KMNAVLGDLISTKARHRGAQGFVIDGLVRDLPTIQELVDFPVFARGVTPIGPLHRGPG
                     EINFPICCGGVVVNPGDIVVGDAMGVVIIPQQFAEELLARLRSHAAANADYYESVKKG
                     NFSNAWVDKILVELECPLIRGVAPSAATPASATPVETAK"
     gene            1122..2267
                     /locus_tag="VLI90_13935"
     CDS             1122..2267
                     /locus_tag="VLI90_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15355.1"
                     /translation="MIDVLTNRWTLRALGVIAMGRAFLRYTNPRRKRSGRAQTAFYER
                     TWREAAEQLGGTCVALGSEICQINVGNQRTRVVHNVCAIDDPVTLAVLHDKPITHRIL
                     QDVGVPVPRHCVFTTKSVAKAAAFLRDLGGDCVVKPAGGTGGGRGVTTGIRTRWQLSR
                     AAAAAAVYADELLIEEQVEGENYRLLYLDGELVDAFVRRRPAVVGDGRSTVAKLVHEH
                     NEQRLKSGTAISQVLLTIDLDMRRTLAKQGLSLRSVPEAGKQVVLKTAINENSGADNS
                     TATHLLCRSIVEDGARAVRALGSRFAGIDLVTRDPALPLAETGGVVIEVNGTPNLYYH
                     YNKSDGVYPAAIEVLKRVLRDIPSSPPEERRVKYPSPVERAAVATVN"
     gene            2363..3643
                     /locus_tag="VLI90_13940"
     CDS             2363..3643
                     /locus_tag="VLI90_13940"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15356.1"
                     /translation="MVSLVNIATKQLVQSPARDASAPHHVASAGERPDAVEQPPVLIL
                     GGEANALSVARDLGRLGAKVFMLGESGTPVRHSRYCNWIELESSAKGSFETIWADFLL
                     GSDSDYLKGAVVLACSDAGITVLATHRDRLSRKFRLDESNTIAQLDMLDKLKTYHHAV
                     AAGVPTPKFWEVHTRADVLAIEKELVYPLLVKPRLSHVFEKHFGRKHVTVTAFEQLLA
                     AYDTAAGAGMDMMLVELIPGGDEQLCSYYTYLDAQGNALVHFTKRIIRRFPTGMGSAC
                     YHITDWNPNLTELGLKLFRHVGLRGLANVEFKQDPRDGVYKLIECNARFTASNCLVSA
                     SGCNLAALVYNRITGRPVKPTTTDYRRGLRLWDPVRDFWAFRELQQRGEITFVQWVSS
                     VMHRQTFPFFRLSDPKPALARTLKPLGRLFRRRS"
     gene            3776..>6220
                     /locus_tag="VLI90_13945"
     CDS             3776..>6220
                     /locus_tag="VLI90_13945"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSV15357.1"
                     /translation="MLRAAAPAGAAILRPDHVVVLVEEERFDNAIGDTAHLPYVNQLA
                     ASSLIFSSVQGLNTPSQLGEMNYLGLYSGSTQGVTDNGRGYTFSGNNIAKAMNNTPGM
                     SFAGYAESLPSDGSQVQQASDPITDPANHPDLYVRNYNPMAMFTSVGTGKVNADVNKT
                     FGHFPSDYTTLPAVSFVIPNTLHNTHGSNESPPFATDPSAYDGLRVAADTWLQQNIDP
                     YVQWAKTHNSLLIITTDELDRQHAGTGVTTMITGDPRLFVPGTNLSPYNHYNTLRTIE
                     DMYGLTPLGSSATVSAFTTNAAGQLVAASSNKHWDGTSNAYLTSHWLDPANVGPYAIS
                     AGDTLTITAGTASFSPASTGQFLLLSSTSTGGQELDVTGGTLSVTTGGPVGAGYGVQL
                     NTGGLLKVTAGTVSIAGPLAIGQTAANAASATFSGGAIVVGATAGTDQTLYVGNAGTG
                     SVVQNGSATVTAPKVALAATVGSTGSYSLQSGTLNAAALTVNTGGTFAWTGGTLATTA
                     ITQNGGSFSATPTLVIAATGGNAASDVLHGGTLSAGAITVNGGGNVAIDSTGNLTATG
                     LTLGATGTTAAVTQSGGSATVTSLVVGSISGAAGSYTLSGGSLASTTTVVGDGGTGAF
                     NQPGGTHAVSTSLTLGKQAGSAGTYTLSGGVLATPSVSGQSGNSTFNFNGGTLRATAG
                     SDSFLSGVKSVIVQSGGATIDTNSNDITITNPIVAGPSSTGGLTKVGTDDLTLGGANT
                     YTGPTAINAGALTLLPGASLASAAITVAAGATFSVAGALATTATVNANGIVNFAGNTP
                     GGSTGVTFSTLNLAANQ"
BASE COUNT         1150 a         2042 c         1985 g         1043 t
ORIGIN      
        1 cgattgcatg aagagatggc cggcgcgtcg tttgccgcga ccggcgaggc gaatgagttt
       61 acggtcgcag cactgatcca gcgcttggcc gacgagggtg tcctcgacgg cggcgccgca
      121 ttgatgggtg aagcgaaatc caagcgtagc acgcgcacga acaaggagtg aagagccatg
      181 gcagtcaaac cacgatccgc acatttgcac gccggtcccg ggtttcgggt gaaggtgaac
      241 tttcagcgtc cgcctcagga gctgatcaag ggcttcgccg attttcccac gccggatatt
      301 tctgatctgc tcaaccgcct gtacgcggtt gaccgcgaaa tccgcgtgct gaccaacccc
      361 gatcatcgcg tctgcggcgc cgcgtgcacc gtgaaggtgt tccccggcga caacctgatg
      421 gtccacaagg cgctcgatgt gctcgagccg ggcgacgtcg tgatcgtcga tgccggcggg
      481 tcgaagatga acgccgtcct cggcgatctg atctcgacca aggcgcgtca ccgcggcgcg
      541 cagggtttcg tgatcgacgg gctcgtgcgc gacctgccga cgatccagga gttggttgac
      601 ttccccgtct tcgcccgcgg cgtcacgccg atcggcccgc tgcaccgcgg ccctggcgag
      661 atcaacttcc cgatctgctg cggcggcgtc gtcgtcaatc ccggcgacat cgtcgtcggc
      721 gatgcgatgg gcgtcgtcat catcccgcag cagttcgctg aagaactgct ggcgcgcctg
      781 cgcagccacg cggcggcgaa cgccgattac tacgagtcgg tgaagaaggg caacttctcc
      841 aacgcctggg tggacaagat cctggtcgag ctggaatgcc cgctgatccg cggcgtcgcg
      901 ccgagcgccg ccacccccgc gtcagcgacg ccagtggaga cggcgaagta agcgcgtgcg
      961 cgaaagatgc ttctcgcgcc cccgctccga ctgatagccg caggcgaaag ccagcggggc
     1021 cgcgagaagt tgaaccggtg ccgtttttcc aagcccccgc tggctatcgc gtgcggctat
     1081 caagggtgtt gtctcgttcg tttgaaggag ttcgcgttcg aatgatcgac gtgctgacca
     1141 accgctggac gctgcgcgcc cttggcgtga tcgccatggg cagggcgttc ctgcgctaca
     1201 cgaacccgcg gcggaagcgt tccgggcgcg cccagaccgc gttctacgaa cgcacctggc
     1261 gcgaggccgc cgagcagctc ggcggcacct gcgtcgcgct gggctccgag atctgccaga
     1321 tcaacgttgg caatcagcgc acgcgcgtcg tgcacaacgt ctgcgccatc gatgacccgg
     1381 tgacgttggc ggtgctgcac gacaagccga tcacccaccg catcctgcag gacgtcggcg
     1441 tgccggtgcc gcggcactgc gtatttacga ccaaaagcgt tgccaaagcc gcggcgtttc
     1501 tgcgcgacct aggcggcgac tgcgtcgtca aacccgccgg tggcaccggt ggcggccgcg
     1561 gggtcaccac cggcatccgc acgcgctggc agctgtcgcg cgccgccgcc gccgctgcgg
     1621 tgtacgccga tgaactgctg atcgaggagc aggtcgaagg tgaaaactat cgcctgcttt
     1681 atctcgacgg cgagctggtt gacgccttcg tccgccgccg cccggcagtc gtcggtgacg
     1741 gtcgatcgac cgtcgcgaag ctcgttcatg aacacaacga gcagcggctc aagagcggca
     1801 cggcaatctc gcaggtgctg ctcacgatcg acctggacat gcggcggacg ctggccaagc
     1861 aggggctttc gcttcggtcc gtgcccgaag cgggcaagca ggtggtgctg aagaccgcca
     1921 tcaacgaaaa cagcggcgca gacaactcga cagcgacgca cttgctctgc cgttcgatcg
     1981 tcgaagacgg cgctcgcgcc gtgcgcgcgc tcggctcccg cttcgccggc atcgatctgg
     2041 tcacgcgcga tccagcgctc ccgctggccg aaaccggcgg tgtcgtcatc gaagtgaacg
     2101 gcacgccgaa cttgtattac cactacaaca aaagcgacgg cgtctatccc gccgcgatcg
     2161 aagtcctcaa acgagtactg cgggatattc cgtcatcgcc gccggaagag cggcgggtga
     2221 aatacccatc gccggtggaa cgagccgcgg tcgctacggt gaattagctt ccatcgagcg
     2281 cgcgcgcccg tctggtcccc tcgaaagagg ggttagggtg agggtgattt tgtcggatcg
     2341 ccaagtgaac agggaaggca gtatggtgtc gctcgttaac atagccacga agcagctggt
     2401 ccaatcaccg gcgcgagatg cgtccgcgcc gcatcacgtc gcctccgccg gcgaaagacc
     2461 ggatgcagtc gagcaaccgc cggtgctgat cctcggtggc gaagccaacg cgctgtcggt
     2521 tgcgcgcgat ctcggccgcc tcggcgcgaa ggtcttcatg ctcggtgaat ccggcacacc
     2581 ggtccgccat tcgcgctact gcaactggat tgaactggaa tcttcggcca agggcagctt
     2641 cgaaacgatc tgggcggact tcctgctcgg ctccgactcc gactatctaa aaggcgccgt
     2701 cgtcctcgcc tgcagcgacg cggggatcac cgtgctggcg acgcatcgcg atcggctgag
     2761 ccgaaagttc cgcctcgatg agtcgaacac catcgcgcag ctggacatgc tcgacaagct
     2821 caagacctat caccacgccg tcgccgccgg cgtgccgacg ccaaagttct gggaagtgca
     2881 cacgcgcgcc gatgtgctgg cgatcgagaa ggagctggtc tatccgctgc tggtcaaacc
     2941 gcggctgtcg cacgtgttcg aaaagcattt cggccgaaag cacgtgacgg tgacggcgtt
     3001 cgagcagttg ctcgccgcgt atgacaccgc cgccggcgcg ggcatggaca tgatgctggt
     3061 cgagctaatc cccggcggcg acgagcaact gtgcagttat tacacgtacc tcgacgccca
     3121 agggaatgcg ctggtgcatt ttacgaagcg gatcattcgg cgctttccca ccggcatggg
     3181 cagcgcgtgc tatcacatca cggattggaa cccgaacctc acggaattgg ggctgaaact
     3241 gttccgccac gtcggtttgc gtggccttgc gaacgtcgaa ttcaagcagg acccgcgcga
     3301 cggcgtgtac aaactgatcg agtgcaacgc tcgattcacc gcatcgaact gcctggtttc
     3361 agccagcgga tgtaatctgg cggcgctggt gtataatcgc atcaccggcc ggcctgtgaa
     3421 gccgacgaca acggattacc gccgcggcct gcggttgtgg gatccggttc gcgacttctg
     3481 ggctttccgg gagttgcaac agcgcggtga aatcacgttt gttcagtggg tgagcagtgt
     3541 gatgcatcgg cagacgtttc cgtttttccg attgagcgat ccgaaacccg ctctggcgcg
     3601 cacgctgaaa ccgcttggcc gactgttccg tcgtcggtcg tgagcgaagg acgttgaaga
     3661 cttcatgtgt tcgccggccg acaggcccgc cgttcatcat ctccgcgctc ctcgcccttc
     3721 acgccgtagc caacggattc tggctgccgc catcggcgca gccgcgggtt tgcttttgct
     3781 gcgcgctgcc gctccggccg gcgccgccat tctccggccc gatcacgtcg tcgtcctcgt
     3841 ggaagaagag cggttcgaca acgccatcgg cgacacggcg cacctgccgt acgtcaacca
     3901 gcttgccgca tcctcgctga tcttcagtag cgtccagggc ctcaacacac cgtcccagtt
     3961 gggcgagatg aactatctcg gcctctattc cggttccacg cagggcgtga ccgacaatgg
     4021 ccggggttac acgttcagcg ggaacaacat cgctaaggcg atgaacaaca cgcctggcat
     4081 gagcttcgcg ggttacgccg agagcctgcc cagcgacgga tcgcaggtgc agcaggcatc
     4141 ggatccgatc actgacccgg ccaatcatcc cgacctgtac gtccgcaatt acaacccgat
     4201 ggcgatgttc accagtgtcg gcacgggcaa ggtgaacgcc gatgtgaaca aaacgtttgg
     4261 tcacttccct tcggattaca cgacgctgcc ggcggtttca ttcgtcatcc ccaacacgct
     4321 gcacaacacg cacggctcga acgaatcgcc gcccttcgcg accgatccca gcgcatacga
     4381 cggcctgcgc gtcgccgccg acacctggct gcagcagaac atcgatcctt acgtgcagtg
     4441 ggccaagacg cacaatagcc tgctgatcat cactaccgac gagctggatc ggcagcacgc
     4501 cggcaccggc gtcaccacga tgatcaccgg tgacccgcga ctgttcgtgc ccggcacgaa
     4561 cctctcgccg tacaaccact acaacacgct gcggaccatc gaggacatgt acggcctgac
     4621 gccgctgggg agcagcgcga ccgtcagcgc gttcaccacc aacgccgcgg ggcaattggt
     4681 ggcggccagc tcgaacaagc actgggacgg cacgagcaat gcgtacctta cgtcgcactg
     4741 gctcgatccg gcgaacgtgg ggccgtatgc gatcagcgcg ggtgatacgc tcacgatcac
     4801 cgcgggcacc gcgagcttca gccccgcttc gaccgggcaa ttcctgctgc tctcgagcac
     4861 aagtaccggt ggacaggaac tggacgtcac cggcggcaca ctgtccgtga cgacgggtgg
     4921 gcccgtcggc gcgggatatg gcgtgcagct taatacgggt ggattgctga aggtcaccgc
     4981 gggcacggtg agcatcgccg gaccgctggc gatcggacag accgccgcca acgcggcgtc
     5041 ggcgacattc agcggtggcg cgatcgtcgt cggtgcgacg gcgggcaccg accagacgct
     5101 gtacgtcggc aacgccggca ccggcagcgt cgtccagaat ggatcggcga cggtgacggc
     5161 gccaaaggtg gcgctcgccg ccaccgtggg gtccactggc agctacagct tgcagagcgg
     5221 aacgctcaac gccgccgcac tcaccgtgaa caccggcggc acgttcgcgt ggaccggtgg
     5281 gacgttggcg acgacggcga tcacgcagaa cggcggcagc ttcagcgcga caccgacgct
     5341 cgtaatcgca gcgacgggtg gcaacgccgc gagcgacgtg cttcacggcg ggactctcag
     5401 cgccggcgcg atcaccgtca atggcggcgg gaacgtcgcg atcgactcca ctggcaatct
     5461 gacggcgacg gggctgacgc tcggcgccac cggcacgacg gccgccgtca cgcaaagcgg
     5521 tgggtcagca accgtcacct cgctggtcgt aggcagcatt tcgggcgcgg cgggatcata
     5581 cacgttgtcg ggcggatcgt tggcgtccac cacgaccgtc gtcggcgatg gtggaacagg
     5641 cgcgttcaat cagccaggtg gcacgcacgc ggtcagcaca tcgctaacgc tcggcaagca
     5701 agcaggcagc gccggcacgt acaccctgag cggcggcgtt ctcgcaacgc caagcgtctc
     5761 gggccagtcg ggcaactcga cgttcaactt caacggcggc acgctgcgcg ccaccgcggg
     5821 cagcgattcg tttttgagcg gcgtcaaatc ggtcatcgtg cagagcggcg gggcgacgat
     5881 cgacacgaac agcaacgaca tcaccatcac caacccgatc gtggccggcc cgtcgagcac
     5941 tggtggattg acgaaggtcg gcacggacga cctgacgctc gggggcgcga acacttacac
     6001 cggtccgacc gcaattaacg ccggtgctct cacgctgctt cccggcgctt cgctggcgag
     6061 tgcggcgatc accgtggcgg ctggcgcgac cttcagcgtc gccggcgctc tggcgaccac
     6121 agcaacagtc aacgccaacg gcatcgtcaa cttcgccggc aacacaccgg gtggatcgac
     6181 gggtgtcacg ttttcgacat tgaacctcgc cgcgaaccag
//
LOCUS       DATIMH010000802         1614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2790424, whole genome shotgun sequence.
ACCESSION   DATIMH010000802 DATIMH010000000
VERSION     DATIMH010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790424"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VLI90_13950"
     CDS             complement(<1..217)
                     /locus_tag="VLI90_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15358.1"
                     /translation="MRVQRAMQSIQATRLETIELRKKVADTIALARSYSDSAKTERIQ
                     ATANLANLRLESSASEAAAPTPSGTPST"
     gene            1076..>1614
                     /locus_tag="VLI90_13955"
     CDS             1076..>1614
                     /locus_tag="VLI90_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15359.1"
                     /translation="MTRRSFLKYGVGTGGLLTVPWVAAPALASTGRRLTKYVEPVPVP
                     GAGIVVATPTSANQYAFTQTQIARRLHPDLPPTPLWAYDDGSGLAGQAGSFGMAVIAQ
                     SGTPVDISFTHRLPDTYPAWLPVDTRETPLGRQVRLMTHLHGGFVAADSDGNPWITPN
                     GFGVGETQTVHYTNQLPQMP"
BASE COUNT          289 a          493 c          549 g          283 t
ORIGIN      
        1 tcgtcgaagg tgttccgctg ggagtaggcg ccgctgcttc gctggccgac gattcgaggc
       61 gcaggtttgc gagattcgct gtcgcctgga tgcgttcggt cttggcggag tcgctatacg
      121 acctcgccag tgcaatcgtg tcggcgacct tcttccgaag ctcgatggtt tcaagccgcg
      181 tcgcctgaat ggactgcatc gcgcgttgaa cgcgcacgcg tgtttcgatc aggtcatccc
      241 aatcgcgatg cggaggcacg catcatcgtg aggcgttcgg ggcgtcagcg caaattcctg
      301 ccgtcttttg tgaagctcgg gccgcggatg acgaccactg ccgcgggcgt cctccgatcg
      361 cgcgcctggc gcgcagaagc cctgcacgtg gtcccgatca gcgggccggc cgccgccgtc
      421 ggtgtgcagg tcgatcgatt cctgtgcggg acggtccgcg ccaggtgcag gccggcgcga
      481 tcaggtgccg ggtggatcgg atcggttgcc agcaggggag aaaccgacgt gtgggccggc
      541 atgaagcgcg tgcgggcggg catcgcacgt tgcaggtaca acgtgctaac tgcttggcga
      601 tcgcaattgc atgcttgtcg aatcgaaatg cgcggcaagg cgctcgagat cggcgttcag
      661 cgcacgcgga actgtgctcg gcggcgccac gcgcgtgctg gccggccggt gatcgctgct
      721 ggagaaaata aatcgtgcaa agtcgccggc gcgcgcgtgg cggtggcaca tgaacgcttg
      781 cttggccggt gtcgtcgcac tgctgtactt atcgaacgcg cgacagcgat aaatgaaaac
      841 gcgaacgtca aacatgaaac cgcgatcagc ggccgtcgaa ccgcgatcat cgaagacgaa
      901 accgacacgt ccgtcgaggt atcagaaggc cagggtgatc actcgccgat actgatatcc
      961 cggacgccgg gaccctgccg ttccccagga ttgcaaccgg ctgttcgcga gcaaggtggt
     1021 aggctcacca tcgctagtgt gagcgtgcag acgatgccga aggggaatgc gtatggtgac
     1081 cagacgatcc ttcctgaaat acggcgtggg caccggtggg cttctgacgg tgccatgggt
     1141 ggcggcaccg gcgctggcgt ccaccggaag aagactgacg aagtacgtgg agcccgtgcc
     1201 tgtccctggc gccggcatcg tcgtcgcgac acccaccagc gcaaatcagt atgccttcac
     1261 gcagacgcag attgcgcgcc ggctgcatcc tgacctgccg ccgacgccgc tctgggccta
     1321 cgatgacggc tccgggctcg ccggccaggc ggggtccttc gggatggcgg tgatcgcgca
     1381 gagtggaaca ccggttgaca tcagcttcac gcaccggctg ccggacacct atcccgcgtg
     1441 gctcccggtc gacacacgag agacgccgct cggaaggcag gtgcgattga tgacgcatct
     1501 gcatggcggc tttgtcgcgg ctgacagcga cgggaacccc tggattacgc ccaacggatt
     1561 cggcgtcggg gagacgcaga ccgttcacta cacgaaccag ctgccgcaga tgcc
//
LOCUS       DATIMH010000803         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2790738, whole genome shotgun sequence.
ACCESSION   DATIMH010000803 DATIMH010000000
VERSION     DATIMH010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790738"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VLI90_13960"
     CDS             <1..651
                     /locus_tag="VLI90_13960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV15360.1"
                     /translation="NRGLSLPDLIEEGNIGLLKAVEGFDPENGSRFSTYASWWIKQAI
                     KRALINSVQPIHIPAYMIEMMSKLKTANRELEDELGRMPSIEELSAHLKMSVKKLKII
                     RKAVKAYHSPTQVGGSGGASGDNELTINDLVADTHNPGPDDQVTNTDELRHLAELLDE
                     IDQRASKILKLRYGLEGEDPMTLKEIGKRIGLTRERVRQIEHEALAKLRDAMMVAV"
     gene            1165..>2069
                     /locus_tag="VLI90_13965"
     CDS             1165..>2069
                     /locus_tag="VLI90_13965"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HSV15361.1"
                     /translation="MNVIDRFQPGELWPDTDGAHINAHGGGVLFHDGSYYWFGEHKVA
                     GEIGNTAQVGVRCYSSTDLYNWTNRGVALAVSDDPASPIARGCVIERPKVIYNPLTRL
                     FVMWFHLEGRDRNYRDAMAGVTVSERVTGPYRFVSAGRVNAGVWPMNAPPEDRRPLDA
                     DESAGLTKLELLGGPSPAYPTNLIYRRDFAGGQMSRDMTLFVDDDGVAYHLHASEENG
                     VLHISQLSEDYQRPAGRYIRVFPGGFHEAPAIFKHAGKYWLITSGCTGWFPNAARLAV
                     ASSIWGPWESLGNPCVGPRAEITFDG"
BASE COUNT          419 a          685 c          643 g          322 t
ORIGIN      
        1 aaccgcgggc tgtcgctgcc ggacctgatc gaggaaggca acatcggact tctcaaagcc
       61 gtagaaggtt tcgatcccga aaacggcagc cgattcagca cctacgccag ctggtggatc
      121 aagcaggcga tcaagcgcgc cctgatcaac agcgtgcagc cgatccacat ccccgcgtac
      181 atgatcgaga tgatgagcaa gctgaagacg gccaaccgcg agttggaaga cgagcttggc
      241 cggatgccca gcatcgaaga gctgagcgcg catctgaaga tgagcgtgaa gaaactcaag
      301 atcatccgca aggcggtgaa ggcgtaccac agccccacgc aagtgggcgg cagcggtggc
      361 gcatccggcg acaacgagct gaccatcaac gacctggtgg ccgacacgca caaccccggc
      421 ccggacgatc aggtgaccaa caccgacgag ctgcgccacc tggccgaact gctggacgag
      481 atcgaccagc gggcgtcgaa gatcctcaag ctgcgctacg gcctggaagg cgaggacccg
      541 atgacgctga aggaaatcgg caagcgcatc ggcctgaccc gcgaacgcgt gcggcagatc
      601 gagcacgagg cgctggcgaa gctgcgcgac gcgatgatgg ttgcggtgta aggttcggcg
      661 cttcacatcc cgaacaaatt taaaaacccc ggccgcgcgc ggtcggggtt tcttcttcaa
      721 atgtcacaca cccccagcgc cggcgtgctc gccaggctcg gcgttggcga gcgagtcatg
      781 ggcgagaagc gccacccccg gccgctcgcg gcttcgcaat ccaggccact cgttggggcg
      841 acagtcatcg gatggtgacg ctcacccggc gcggcctcag caacacgctt cgtgaatccg
      901 ccgccgcacg cgccgggtcg gacgcagcag caacccacca gcgcccggta tgattgccac
      961 ccccacccgg ctccggcacg gccacgcttg acgctgaccc gcccgatggg gccgacttaa
     1021 cagatgggct cacatcgatt attacccgcg ccatcgcacg ggtaaaacgt cgatcgtttg
     1081 ttcgtgaagc cagtgtgaga gattgcccac gcgcacttgg cagattgacc cccagccttg
     1141 cggcccgggg cgatgaaaga accgatgaat gtgatcgacc gatttcaacc tggggaactg
     1201 tggcccgaca cggatggcgc gcacatcaac gcccacggcg gcggtgtgct ctttcacgat
     1261 ggcagttact actggttcgg cgagcacaaa gtcgcgggcg agatcggcaa caccgcacag
     1321 gtgggcgtcc gctgctactc gtccaccgat ctatacaact ggaccaaccg cggcgtcgcg
     1381 ctggccgtca gtgatgaccc ggcaagcccc atcgcgcgcg gctgcgtgat cgagcgccca
     1441 aaggtgatct acaacccgct cacgcggctg ttcgtgatgt ggttccacct cgaaggccgc
     1501 gatcgcaact atcgcgatgc gatggcgggc gtcaccgtga gtgagcgcgt cacggggccg
     1561 tatcgcttcg tctccgcagg ccgagtcaac gcgggcgttt ggccgatgaa cgcgccgcca
     1621 gaagatcgtc gcccgctcga tgccgatgaa tcggcgggcc tgacgaaact ggaattgctc
     1681 ggcggaccgt cgccggcgta tccgaccaac ctgatctatc gccgcgattt cgccggcggg
     1741 cagatgtcgc gcgacatgac gctgttcgtc gatgacgatg gcgtcgcgta tcacctgcac
     1801 gcgtccgaag agaacggcgt cctgcacatc tcgcagctca gcgaagatta ccagcgcccc
     1861 gccgggcgat acattcgcgt cttccccggc ggcttccacg aagcccccgc gatcttcaaa
     1921 cacgcgggaa aatactggct catcaccagc ggttgcaccg gctggttccc caacgccgcc
     1981 cgattggccg tcgcgtcgtc catctggggc ccgtgggaat cgctcggcaa tccctgcgtc
     2041 ggcccgcggg cggaaatcac cttcgacgg
//
LOCUS       DATIMH010000804         1886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_482244, whole genome shotgun sequence.
ACCESSION   DATIMH010000804 DATIMH010000000
VERSION     DATIMH010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482244"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VLI90_13970"
     CDS             <1..317
                     /locus_tag="VLI90_13970"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HSV15362.1"
                     /translation="DLDRYEADGIHNDPRFTRDKMADALAVTDFMAWAKESNALAAHY
                     AYLDGQLKGAVQSRDRSAPREDIPGVPPGYIANAELVSMHQVALAGHRLADLLNRLYD
                     AK"
     gene            complement(452..>1886)
                     /locus_tag="VLI90_13975"
     CDS             complement(452..>1886)
                     /locus_tag="VLI90_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV15363.1"
                     /translation="RTAPAGFTSVAPIAEYTHSDGEAIIGGDVFRGSGFSSLVGKFVF
                     GDLGGVASNTIGRLFYTDAAGGTVSEFNYDSSGITPGANLYGVTNGPNNELYALFSNG
                     AIVSLTAGIFTTQWNLSTGSTWGTAGNWTNGLPTPQASHANFLASPGVTTASIVTLDG
                     NRTVGQITFNNANGYTIAQGSGGTLTIDDSNDAGGVNPLVNVIAGSHTISAPISLLNG
                     LSISAASGTAINITGSISGTGSLTRNAGTGVLTIGGKMSITGNVNANGPLNFGGNTST
                     AALTHTFAGLNVGPGVTVIANTSANAFVPTVLHGITLSFGDSTAKIDLKNNELQSTMT
                     LAQIENFLFGSPAHIVTTTGGPGELGYADTGGGVTEVRYTLPGDTNLDGTVDVGDLGA
                     LATNYGSSSGVAWQQGDFDHSGVVNVGDLGALATSYGNSIGTGPADASATSTTAAAAV
                     PEPTAGALMLAGAAALLRRRSRHHLLK"
BASE COUNT          314 a          596 c          617 g          359 t
ORIGIN      
        1 acgatctcga tcgatacgaa gccgacggca ttcacaacga cccgcgtttc acgcgcgaca
       61 agatggctga cgccctggcc gtcacagact tcatggcctg ggcgaaagaa agtaacgcct
      121 tagccgccca ttacgcgtat ctcgacggcc agctcaaagg cgcggttcag tcacgcgatc
      181 gctccgctcc ccgcgaagac atccccggcg ttccccccgg ttacatcgcg aatgccgagc
      241 tggtgtcgat gcaccaggtc gccctcgccg gtcaccgcct ggcggatctg ctcaatcgcc
      301 tctacgacgc gaagtaacgg atgtgcccat caccaaatcc gaccgggctg accgcttcag
      361 cggtcagcac ttcggcgtca tcacaatcat ccaggtcgcg cgcgagttcg cgcgagtagt
      421 cctcaaataa gaggccgcac ccgatcgttc actatttgag caagtgatgc cgggaacggc
      481 gccgcaacag agccgcggcg ccagcgagca ttagagcacc ggccgtcggt tccggtaccg
      541 ccgcggcggc ggtggtcgag gtcgcgctcg catccgcggg cccggtgccg atgctgttgc
      601 cgtagctggt cgccagcgcg cctaggtcgc ccacgtttac cacgccgctg tgatcgaagt
      661 cgccctgttg ccacgcgacg ccgctgcttg agccatagtt ggttgcgagc gcgcccaggt
      721 cgcccacgtc taccgtgccg tcgagattcg tatcacccgg cagcgtatag cgtacctccg
      781 ttacaccgcc gccggtgtcg gcgtagccga gttcgccggg gccgcctgtc gtggtgacga
      841 tgtgcgccgg cgagccgaag aggaagttct cgatctgggc cagcgtcatc gttgactgca
      901 actcgttgtt cttcagatcg atctttgcgg tggaatcgcc gaagctcagc gttatgccgt
      961 gcagcaccgt ggggacgaag gcgttggccg atgtgttggc gatgaccgtc acgccagggc
     1021 cgacgttgag ccctgcgaac gtatgcgtca gtgccgcggt gctagtattc ccgccgaagt
     1081 tgagggggcc gttggcgttc acgttgccgg tgatcgacat cttgccgccg atcgtcaaaa
     1141 cgccggtgcc ggcgtttctt gtcagcgaac ccgtgcccga aatgctgcca gtgatgttga
     1201 tcgctgtgcc cgaggcggcg ctgatcgata atccattcag caatgagatc ggcgccgaga
     1261 tggtgtgact gccggcgatg acgttgacca gcgggttgac accgccggcg tcgttggagt
     1321 cgtcgatcgt cagcgtgcca ccgctgccct gggcgatcgt gtagccgttg gcgttgttga
     1381 acgtgatttg cccgacggtg cggttgccat cgagcgtgac gatgctggcc gtggtgacgc
     1441 cgggcgacgc gaggaagttc gcgtgcgatg cctgcggcgt cggcaggccg ttggtccagt
     1501 tgccggcggt gccccaggtg ctgccggtgc tcaggttcca ttgcgtcgtg aaaatgccgg
     1561 ccgtcaggct gacgatcgcg ccgttgctga acagcgcgta cagctcgttg ttcggcccgt
     1621 tggtgacgcc atacagattc gcgccgggcg tgatgccgct cgagtcgtag ttgaattccg
     1681 acaccgtgcc cccggcggcg tcggtgtaga agagccggcc gatcgtgttg ctggcgacgc
     1741 caccaagatc accgaagacg aacttaccga ccagcgaaga aaaaccggac ccgcggaaga
     1801 cgtcgccgcc gatgatcgct tcgccgtcgc tgtgggtgta ctcggcgatc ggcgcgactg
     1861 acgtgaatcc ggccggcgcg gtgcgt
//
LOCUS       DATIMH010000805         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5394966, whole genome shotgun sequence.
ACCESSION   DATIMH010000805 DATIMH010000000
VERSION     DATIMH010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5394966"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /gene="tilS"
                     /locus_tag="VLI90_13980"
     CDS             complement(<1..919)
                     /gene="tilS"
                     /locus_tag="VLI90_13980"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HSV15364.1"
                     /translation="MPEVPTRLSAAIAAVPAGRWAVAVSGGADSVALLMLLARDARAS
                     ERMLHIVHLDHQARAGASGEDAAFVENLATRFHLPCTIARRSEIEAGMVDLPNNTAAR
                     YRAVRMRLFRDVIESHQLQGVIVAHHGDDQAETVLHRLLRGSPPTALAAMSVDAVING
                     VRVLRPLLAVPHVDLQRYLEGIGETWREDASNASPRYLRNRIRPILAKDSALRGSAIR
                     LGSACSALKAWLNHVMPGLDDRFDAAKLGSLPPPLARAAASRWLAHHAVPVEEISQVV
                     CDRLIAMATDAASPARQHFPGGVQVHRRRG"
     gene            1149..1664
                     /locus_tag="VLI90_13985"
     CDS             1149..1664
                     /locus_tag="VLI90_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15365.1"
                     /translation="MNQTAPTTAPVTQPAAPFEPAAGEPDAARPGVIQLSDGATVRGS
                     IATTPEKPVRVWTEEDKEYQDIPLAMIKSIDADVLWERDEREWRFKAGGSDVKEYSGK
                     TYPARETRYKLTLIDGTTITGGIVAPLYVSVDGQTKTLVLHKRDKGNPGQTLAQLLYV
                     KAVRFEDAPHP"
     gene            1774..>2220
                     /locus_tag="VLI90_13990"
     CDS             1774..>2220
                     /locus_tag="VLI90_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSV15366.1"
                     /translation="MNHPSQPLITLIALLLSTSLFAADPPPVVKTSPPSAATAEKVLK
                     DSPRHGEWVDVPMPGSEVKLHSWIVYPERSDKAPVVIVIHEIFGMSDWVRATTDQLAA
                     EGFIAVAPDLLSGFGPNGGGTESFNDRDVRQAIMKLSPDEVAKDLDA"
BASE COUNT          427 a          786 c          669 g          338 t
ORIGIN      
        1 ccccgcgccg ccggtgcacc tgcacgcccc ctggaaaatg ctgccgcgct ggcgatgcgg
       61 catccgtcgc catcgcgatc aagcgatcgc aaacaacctg gctgatctcc tcaaccggca
      121 ccgcgtgatg cgcgagccag cgcgacgccg ccgcccgcgc cagcggcggc gggaggctcc
      181 ccagcttcgc tgcatcgaag cgatcatcga gcccgggcat cacatgattc aaccacgcct
      241 tcagcgctga gcacgcggag ccaagacgga tggcgctgcc gcgcagtgct gaatccttcg
      301 ccaggatcgg tcggattcga ttgcgcaaat atcgcggcga tgcgttgctc gcatcctcgc
      361 gccaagtctc gccaattccc tcgagatacc gctgcagatc gacatgagga acggccagca
      421 gtggccgcag cacgcgcacg ccgttgatga cggcatcgac cgacatcgcc gccagcgctg
      481 tcggcggcga gccgcgcagc aggcggtgaa gcaccgtctc cgcctgatcg tcgccgtggt
      541 gggcgacgat gacgccttgc agttggtgag attcgatcac gtcgcgaaac agccgcatgc
      601 ggaccgcacg atatcgggcg gcggtgttgt tcggcagatc gaccatgcct gcttcgatct
      661 cgcttcgtcg cgcgatcgtg cacggcaggt gaaaacgtgt cgccagattt tcgacaaacg
      721 ccgcgtcttc accgctggcg ccggcgcgcg cctggtgatc gaggtggaca atatgaagca
      781 tccgttcact cgcgcgggcg tcgcgcgcca gcaacatcaa caacgcgacg ctatccgccc
      841 cgccgctgac ggcgacggcc caccgtcccg ctgggacggc ggcaatggcc gcagacaatc
      901 gcgtcggaac ctcgggcatt gcggtatcat cgaacgccgt gatggcaaac cgcaagcgcc
      961 acagcgcacg tcaatccccg cgtagcggta gccccgcagg ggcacgtgct tttcgttcaa
     1021 gcgcaaaaac acgtgcccct gcagggctac cgctacgcgg agggctttac gtttttctgt
     1081 tcacctgcgc cgtcccgccc gcacccggcg ctccgccgcc ggcaacggac ccgaccatcg
     1141 actggctcat gaatcaaacc gcgccgacga cagcgccggt gactcagccg gcggcgccat
     1201 tcgaacccgc cgcgggcgaa cccgacgccg cgcgtccggg cgtcatccag ttgagcgacg
     1261 gcgccaccgt ccgcgggtca atcgcgacca cgccggaaaa acccgtccgc gtgtggaccg
     1321 aggaggacaa ggaatatcag gacattccgc tggcgatgat caagtcgatc gacgccgacg
     1381 tgctgtggga gcgcgacgag cgcgagtggc gtttcaaagc cggcggcagc gacgtcaagg
     1441 aatacagcgg caagacctat cccgcgcgcg agacgcgcta caagctcacg ctgatcgacg
     1501 gcaccacgat caccggcggg atcgtcgcgc cactgtatgt gagcgtcgat ggccagacca
     1561 aaacgctggt cctgcacaaa cgcgacaagg gcaaccccgg ccagacgctc gcgcagctgc
     1621 tgtacgtgaa ggccgtgcga ttcgaagacg caccgcatcc ctgaacgctt cacgccaacc
     1681 cccgcgccgg gttcggagcg tactcgcgac gacccggatg ccttttgtct cgcagttgcc
     1741 aactcgacgg atcgtcaaca caatcgcacc attatgaacc acccatctca accgctcata
     1801 acactgatcg cactgctcct ctccacaagc ctcttcgccg ccgacccccc tcccgtggtg
     1861 aagaccagcc caccgtcggc tgccactgcg gaaaaggttc tgaaagattc gccgcgccac
     1921 ggcgaatggg ttgacgtgcc gatgcccggg tcggaggtga agctgcacag ctggatcgtc
     1981 taccccgagc gcagcgacaa ggcgcccgtc gtcatcgtca tccacgagat cttcggcatg
     2041 tccgactggg tccgcgccac caccgaccaa ctggccgccg agggattcat cgccgtcgcg
     2101 ccggacctgc tcagcggttt cggccccaac ggcggcggga ccgaatcgtt caacgatcgg
     2161 gatgttcgcc aggcaatcat gaagctctcg cccgatgaag tcgccaagga tctcgacgcc
//
LOCUS       DATIMH010000806         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2212615, whole genome shotgun sequence.
ACCESSION   DATIMH010000806 DATIMH010000000
VERSION     DATIMH010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212615"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VLI90_13995"
     CDS             <1..650
                     /locus_tag="VLI90_13995"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HSV15367.1"
                     /translation="PYHNGRRARPRSWITDFPDSDSNFSFRLQQMTSIKTDPHGLIIE
                     LTDPRLFDYPFIYMLEVGSLEFTDEEMVALRRYLLNGGFLMVDDHWGVAEYENFHTEL
                     LRLFPGREPVELPLEHPIFHCVFDLKFKPQIPGIDVARRVRGTNITWEREDAKEPHYR
                     AVFDDKGRIMILICSNTDLGDGWEREGEEEWYFHQFSEKQAYPMGINIVFWAMTH"
     gene            728..1732
                     /locus_tag="VLI90_14000"
     CDS             728..1732
                     /locus_tag="VLI90_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSV15368.1"
                     /translation="MTTDTTAYDIERKTVEQLREARERIHRELSKAVVGQKDVTEQLL
                     YCLFAGGHCLITGAPGLAKTLLVRSLAQIFHLKFQRIQFTPYLMPADITGTEILEETG
                     DGKRRMQFVKGPIFANVLLADEINRTPPKTQAALLEAMQEYQVTAGGVRYPLEEPFFV
                     LATQNPIEMEGTYPLPEAQLDRFMFNVVIDYLPQDDEVEVVKRTTSVKPEPIQPMFTG
                     EDVLNFHNVVRRVPIAEDIVRYAVRMASASRPGQADTPDFITQWVSWGAGLRAAQYLV
                     LGAKARAILAGRAHVSADDVKALVHATFRHRILINYRAEAEGVNVEKVIDRLVETLKP
                     "
BASE COUNT          378 a          614 c          567 g          318 t
ORIGIN      
        1 ccccctatca caatggccgg cgcgcccggc cgcgctcgtg gatcaccgac ttccccgaca
       61 gcgattccaa cttctcgttc cgcctgcagc agatgacttc gatcaagacc gacccgcacg
      121 gcctgatcat cgagctgacc gacccgcggc tgttcgatta cccgttcatc tacatgctcg
      181 aagtcggctc gctcgaattc accgatgagg agatggtcgc actccgccga tacctgctga
      241 acggcggatt cctgatggtc gacgaccatt ggggcgtcgc cgaatacgag aactttcata
      301 cggagctgct gcggctcttc cccggccgcg agccggtgga actgccgctg gagcatccaa
      361 tctttcactg tgtgtttgat ctgaagttca aaccgcagat ccccggcatc gacgtcgccc
      421 ggcgagtccg cggcaccaac atcacgtggg agcgcgaaga cgcgaaggag ccgcactatc
      481 gcgcggtctt cgacgacaag gggcggatca tgatcctaat ctgctcgaac accgacctgg
      541 gcgacgggtg ggaacgcgaa ggcgaagagg aatggtactt ccaccagttc tcggagaagc
      601 aggcgtaccc gatggggatc aacatcgtgt tctgggcgat gacgcattga tgtagcacgg
      661 gcgtctcgcc cgtgccgagc gtgctggaca cgggcgagac gccgtgctac ttcagaacgg
      721 agatcgagtg accaccgaca ccaccgcgta cgacatcgag cgcaagaccg tcgagcaact
      781 ccgcgaagcg cgcgagcgca tccaccgcga gctgtccaaa gccgtcgtcg gccagaagga
      841 cgtcaccgag cagctgctct actgcctttt cgccggcggg cattgcctga tcaccggcgc
      901 gccgggtctg gccaagacgc tcttggtccg atcgctcgcg caaatcttcc acctcaagtt
      961 ccagcgcatc cagttcacgc cgtacctgat gccggccgac atcaccggca cggagatttt
     1021 ggaagaaacc ggcgacggca aacgccggat gcagttcgtg aagggaccga tcttcgccaa
     1081 cgtgttgctg gccgacgaga tcaaccgcac gccgcccaag acgcaggcgg cgttgctcga
     1141 agcgatgcag gaatatcaag tcaccgcggg cggcgtgcgc tatccgctgg aagaaccgtt
     1201 cttcgtgctg gccacgcaaa acccgatcga gatggaaggc acctatccgc tgcccgaagc
     1261 gcagctcgac cgcttcatgt tcaacgtagt aatcgactac ctgccgcagg acgacgaggt
     1321 ggaagtcgtg aaacgcacga ccagcgtgaa gccggaaccg atccagccga tgttcaccgg
     1381 cgaggacgtg ctgaacttcc acaacgtcgt ccgccgggtg ccgatcgcgg aggatatcgt
     1441 caggtatgcg gtgcgtatgg catccgccag ccgtcccggg caggcggata cgcccgactt
     1501 catcacgcaa tgggtcagct ggggcgccgg cctgcgcgcg gcccagtacc tggtgctcgg
     1561 ggccaaggcc cgcgccatcc ttgccggtcg cgcccatgtg tcggccgacg acgtcaaagc
     1621 gctcgtccac gcgacgttcc gccaccgcat cctgatcaac tatcgcgccg aagccgaagg
     1681 cgtgaacgtg gagaaagtga tcgatcggct ggtggagacg ctgaaaccgt aacggccatt
     1741 tgccaatcgc acgcgcgaaa acaggtttcg tagcctaccc ggcacatgca ctacctcgcc
     1801 cccatcccga tcatcatcgg cgtcttcggc gcgatcatgg gtttgatgtg gctcgtcgcc
     1861 gccggggcgg acgacga
//
LOCUS       DATIMH010000807         3480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5106837, whole genome shotgun sequence.
ACCESSION   DATIMH010000807 DATIMH010000000
VERSION     DATIMH010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5106837"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VLI90_14005"
     CDS             <1..1047
                     /locus_tag="VLI90_14005"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSV15369.1"
                     /translation="AIKAQPLMSPRHLRVLITFLSLAMIGCQQQAQPTDKKSYMAPPP
                     KPPPAVPPVTPTPIDPALQAAARQQLDESLRSSDEFVRAHAIEMIKQVNAPDAGEKIV
                     AALGDSSPLVRKAAAFAAGELRVQAAMDRLDLSLSTAKLPEQMAIIFALHRLGDTSRS
                     HIFEQTATDPNEHVRGDTAFILGLLGERSALGILDQMLRSDRSAPVRLQAAEAMWKLG
                     DERGENELINATVSMYPDDKIIALLALAQPKDTRVLGHVEGLLTDDYPEVALMAARAT
                     GMLGSDRGYGVALNGAKSVDPRQRVLAAMALGAIGRADSQPVLAKLLKDDDADTRLAA
                     AGALLQLGTRAQNK"
     gene            complement(1101..1364)
                     /locus_tag="VLI90_14010"
     CDS             complement(1101..1364)
                     /locus_tag="VLI90_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15370.1"
                     /translation="MSDREIIYKLLFDALLELRIEGHESKNAKVFRLADLFQQLPNRL
                     RDMQQGKIEESQILEELRQRARERGVEQWLDLHLPQLVGDQSD"
     gene            complement(1475..1663)
                     /locus_tag="VLI90_14015"
     CDS             complement(1475..1663)
                     /locus_tag="VLI90_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15371.1"
                     /translation="MRVKVKDLDPLHSPDVSGDRPRLRCLTDAELLDACQRPHNGDRI
                     KIGSNSGKVVDGNGRVYN"
     gene            complement(1694..>3480)
                     /gene="glmS"
                     /locus_tag="VLI90_14020"
     CDS             complement(1694..>3480)
                     /gene="glmS"
                     /locus_tag="VLI90_14020"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HSV15372.1"
                     /translation="DSAGIAVIDDRGGMHIRRAVGRISVLESALQNGNSLPDAHVGMA
                     HTRWATHGAPTEINAHPHTDNAGRIALVHNGIIENYAALKKFLTEKGHSFTSQTDTEV
                     LAVLIGDLYEDIKQKNHVPAGVSILQRAVQAALHEVDGTYGIAVVCKEEPDTLVVARK
                     GSPLIVGVGDDEYVVASDASAIVEHTRQVIYLNDNEMAVLKPGSVRTMTIDDIHVNST
                     ISQLENKLEEYELGEYEHYMLKEIFEQPTAIRNCLRGRAEQREGRIVFGGLSDRTRDL
                     VRARRFILTAQGTAWHAGMIGDYLLEDLAKVPAECTYASEFRYRNPLVEENSVIVAIS
                     QSGETADTLAALREGKEKGALAIGIVNVVGSTIARETDGGIYLHAGPEIGVASTKAFT
                     CQMTVLTMLSLFVGRRKFMSQGQMQEIIDGLCAIPEQMEQVLKQSEVVRDIAKEFVDR
                     ENWLFLGRGYHYPIALEGALKLKEISYIHAEGMPAAEMKHGPIALINEGMPVVFVATK
                     GLQYDKVISNIEEVRARGGRVIAVATEGDTAIRRHSDHVVYVPDTVECLQPLLSVVPL
                     QLLAYHAAVARGCNVDKPRNLAKSVTVE"
BASE COUNT          606 a         1081 c         1108 g          685 t
ORIGIN      
        1 gctatcaagg cgcaaccact catgtcacca agacatctcc gcgtgctcat cacgttcctg
       61 tcgctcgcga tgatcggttg ccaacagcag gcccaaccga ctgacaagaa gagttacatg
      121 gcccccccgc ccaagccgcc cccggcggtg ccgccggtga caccgacgcc gatcgaccct
      181 gctttgcagg ccgccgcccg acagcaactg gatgagtcgc tgcgctccag cgacgaattc
      241 gttcgcgcgc acgcgatcga gatgatcaag caggtgaatg cgccggatgc cggagaaaaa
      301 atcgtcgcag cgctgggtga ttcgtcgccg ctggttcgca aagccgcggc gtttgccgcg
      361 ggggaactgc gcgtgcaggc ggcgatggac cgattggacc tgtcgctcag caccgcgaag
      421 ctgccggagc agatggcgat catcttcgcg ctgcatcggc tgggtgacac cagccgatcg
      481 cacattttcg agcagaccgc gacggatccg aacgaacacg ttcgcggcga caccgcgttc
      541 atcctggggc tgctgggcga gcgcagcgcg ctgggcatcc tcgaccagat gctgcggagc
      601 gaccgcagcg cgcccgtgcg acttcaggcg gccgaggcga tgtggaagct gggcgatgaa
      661 cgcggcgaaa acgaattgat caacgcgacc gtcagcatgt atccggatga caagatcatc
      721 gcgctgctcg cgctggcgca gccgaaggac acgcgggtgc tcgggcacgt cgaagggctg
      781 ctgacggatg attacccgga ggtcgcgctg atggccgccc gggccacggg gatgctcggc
      841 agcgaccgcg gatatggcgt ggcgctaaac ggcgccaaga gcgtagaccc gcgccaacgc
      901 gtgcttgcgg cgatggcgct gggggccatt ggtcgagccg attcacagcc ggtgctggcg
      961 aagctgctga aggatgacga tgccgacacg cggctcgccg ccgcgggagc gctgctgcag
     1021 ttggggacga gggcgcaaaa taaatgaaga aacgccggta tggactacgc tgcgtcccgc
     1081 cgtgcagttg acgaggcgtt tcaatctgat tggtcaccga cgagctgggg tagatgcaag
     1141 tcgagccact gctcaacgcc acgttcgcgg gcgcgttggc gcagctcctc aaggatctgg
     1201 gactcttcga tcttcccctg ctgcatgtct cgcaggcggt tcggaagctg ctggaacaaa
     1261 tccgccaggc gaaatacttt cgcattcttc gattcgtgcc cctcgatccg gagctcgagc
     1321 agggcatcga acagcagctt ataaattatt tcgcggtcgc tcatggtgct cgcctattct
     1381 agctccggaa acatcgagcg gtcaggcgaa taaagctcac attcaatttc ggtgtcggcg
     1441 gtgatcgacc ggctatccaa agtcgcgcga ggctttaatt atagacgcgt ccattcccat
     1501 cgacgacctt gccgctgttg ctgccgatct tgatgcggtc accgttgtgc gggcgctgac
     1561 aagcatcgag cagttcggcg tctgtcaggc accgcagtct cggtcgatcg ccggaaacgt
     1621 ccggcgaatg cagcgggtcc aaatctttca ccttgacccg caaggcatcc tcccaggccg
     1681 ccaccgatca ccatcactcc accgtcacgc tcttggcgag gttgcgcggc ttatcgacgt
     1741 tgcacccgcg cgccacggcg gcgtgatatg cgagcagttg tagggggacc acgctcagca
     1801 acggttgcag gcattctacc gtgtcgggga catagacgac gtgatcgctg tgccggcgga
     1861 tggcggtgtc gccttcggtg gcaacggcga tcactcttcc gcctctggct cgcacttctt
     1921 cgatgttgct gatgacctta tcgtattgca aacctttggt cgcgacgaag acgacgggca
     1981 tgccttcgtt gatcagcgcg atcgggccgt gcttcatttc cgccgcgggc atgccttcgg
     2041 cgtggatgta gctgatttcc ttcagcttca gcgcgccttc cagcgcgatc gggtagtgat
     2101 acccgcggcc gaggaacagc caattttcgc ggtcgacgaa ttccttggcg atgtcgcgca
     2161 cgacttcgct ctgcttcagc acctgttcca tctgctcggg gatcgcgcac aggccgtcga
     2221 tgatctcctg catctggccc tggctcatga actttcgccg gccgacgaac agcgacagca
     2281 tcgtcagcac ggtcatctgg caggtgaagg ccttggtgct ggcgacgccg atttccggac
     2341 cggcgtgcag gtagatgccg ccgtcggtct cgcgggcgat ggttgacccg acgacgttga
     2401 cgatgccaat agccagcgcg cctttttcct tgccttcgcg cagcgcggca agcgtgtcgg
     2461 cggtttcacc cgactggctg atcgcgacga tgaccgagtt ttcttccacc agcggattgc
     2521 gataacggaa ttcgctggca taggtgcatt ccgccggcac cttggcgagg tcttccagca
     2581 gataatcgcc gatcatgccg gcgtgccacg cggtgccttg ggcggtgagg atgaaccggc
     2641 gggcgcggac tagatcgcgc gtgcgatcgg acaaaccgcc gaacacgatc cggccttcgc
     2701 gctgttcggc gcggccgcgc aggcagttgc ggatggcggt gggctgctca aaaatctcct
     2761 tgagcatgta atgttcgtac tcgccaagct cgtactcctc gagcttgttc tccagctgcg
     2821 agatggtcga gttcacgtgg atatcgtcga tggtcatcgt gcggacgctg cccggcttca
     2881 gcacggccat ctcgttgtcg ttgaggtaaa tgacctggcg cgtgtgctcg acgatggcgc
     2941 tggcgtcgga ggcgacgacg tattcgtcat cacccacgcc gacgatcagc gggctgccct
     3001 tgcgcgcgac gaccagcgtg tccggttcct ccttgcagac gaccgcgatg ccataggtgc
     3061 cgtccacttc gtgcaacgcc gcctgcaccg cgcgctgcag gatcgacacg cccgcgggca
     3121 cgtggttctt ctgcttgatg tcttcgtaca gatcgccgat cagcacggcc agcacttcgg
     3181 tgtcggtctg gctggtgaag ctgtggccct tctcggtgag gaacttcttg agagccgcgt
     3241 aattttcgat gatgccgttg tgaaccagcg cgatgcgacc ggcgttgtcg gtgtgcgggt
     3301 gagcgttgat ttccgtcggc gcgccatgcg tcgcccagcg cgtgtgggcc atgccgacgt
     3361 gggcgtcggg caggctgttg ccgttctgca aagcgctttc cagaacgctg atccgcccca
     3421 cagcccggcg gatgtgcatg cccccgcggt catcgattac cgcaatgccc gccgagtcat
//
LOCUS       DATIMH010000808         2842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5681556, whole genome shotgun sequence.
ACCESSION   DATIMH010000808 DATIMH010000000
VERSION     DATIMH010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5681556"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1173
                     /locus_tag="VLI90_14025"
     CDS             130..1173
                     /locus_tag="VLI90_14025"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSV15373.1"
                     /translation="MAHKYPALHNAMWPGLVGKGGPGAEPPIDLDTMLDMTAKASSNG
                     TKFDGVDLFLFDPHVSIDSTDDDLKRLADKIRSKGLGVGSLVAPVWSPTGGGSAMGSD
                     EERKNFVTQVRKACAIAKKLKDIGIRKYGVVRIDSAASPSEWAKDPAGSTKKIAETFR
                     QACDVAEQYGERLAAEGEICWGGMHSVKRNVELMEMVNRPKTLGFQADMAHTLLFTMG
                     YNAPDDRILPENFDWSDRKTLDEGLKTMTRALRPWTIDFHVAQNDATVKGSGSHDKTG
                     RHCLPNDPNGKLDIVRHAGFWLKDDSGTPTRAVQHICWDGCMFPNATMTQQKTWNDIL
                     SVMVAVRDAHGWD"
     gene            1331..2461
                     /locus_tag="VLI90_14030"
     CDS             1331..2461
                     /locus_tag="VLI90_14030"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV15374.1"
                     /translation="MKNLNIGLIGYGFMGRTHSNAFLQAPRFFDLGCKPVLKAVAARN
                     ADRVKQFADNWGYESYDTDWRNLVARKDIDVIDIASPNDTHHEIAIAAAKAGKMVMCE
                     KPLGRTAKEAKEMVEAVEKAGVPNTVWYNYRRVPAVTLIKQLLDEGKFGRIFHYRAKF
                     LQDWTISQDLPQGGEGLWRLDVAVAGSGVTGDLLAHNIDTALWLNGPITEVTAMTETF
                     VKQRKHNLTGKVEPVGIDDASAFLCRFENGSLAMFEATRYARGHKALYTLEINGEHAS
                     AFWDLHDLHRIQYFDHRDEGRVRGWRSVHVTDGDHPYMKHWWVPGLQIGYEHTFIHQF
                     ADFAVAAIKGQNIAPTFRDGLATDYVTDAVLKSAKTGKWEKV"
     gene            2510..>2842
                     /locus_tag="VLI90_14035"
     CDS             2510..>2842
                     /locus_tag="VLI90_14035"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HSV15375.1"
                     /translation="MLWPGTCAAIVFFCVVVPGAQQQPNPPAPQQPPAGQPPAGAPGA
                     PAQGRGRGGGGFRQPDPIDFQEHEGWTALFDGKTLNGWSGDNNWKVEDGAITIESTCE
                     KPTGTIYLV"
BASE COUNT          623 a          827 c          924 g          468 t
ORIGIN      
        1 ccccggaaat cctgacgccg aggcgtttag aaagaagtaa ttttccggga tgcgttgtcc
       61 gtgatccgcg atccgaacgg agcccggatc ccggatcccg gaccccgaaa cctacagcga
      121 gagtaaccaa tggcgcataa ataccccgct ctccataacg cgatgtggcc gggacttgtc
      181 ggcaaaggcg gtccgggagc cgagccgccg atcgatctcg acacgatgct cgacatgacc
      241 gccaaggcgt cgtcgaacgg cacaaagttc gacggcgtcg acctgtttct gttcgatccg
      301 cacgtcagca tcgactcgac cgacgacgac ctgaaacgtc tggcggacaa aatccgcagc
      361 aaggggctgg gggttggatc gctcgtcgcc cccgtgtggt cgccgacggg cggcggatcg
      421 gcgatgggca gcgacgagga gcgcaagaat tttgtcactc aggtccgcaa ggcatgtgcg
      481 attgcgaaga agctgaaaga cataggcatt cgcaaatatg gcgtggtccg gatcgactcg
      541 gccgcgagtc cgtccgagtg ggcgaaggat cctgcgggca gcacgaagaa gattgccgag
      601 accttccgtc aagcatgcga cgtggcggag caatacggcg aacggctggc cgccgagggt
      661 gagatttgct ggggtgggat gcacagcgtc aagcgcaacg tcgagctgat ggaaatggtc
      721 aaccgtccaa agaccctggg tttccaggcc gacatggcgc acacgctgct cttcaccatg
      781 ggctacaacg cgcctgacga tcgaatcctg ccggaaaact tcgattggtc cgatcgaaaa
      841 acgctcgacg aagggctgaa gacgatgacc cgggccctgc ggccgtggac gattgatttc
      901 cacgtggcgc aaaacgatgc gacggtcaaa ggatcaggct cacacgacaa gactggccgg
      961 cactgcctgc ccaacgatcc aaacggcaag ctcgatatcg tccgtcatgc cggattctgg
     1021 ctgaaagacg atagcggcac accgacgcgc gcggtgcagc acatctgctg ggacggctgc
     1081 atgttcccga acgccacgat gacgcagcag aaaacgtgga acgacattct gagcgtgatg
     1141 gtggcggtgc gggatgcgca cggctgggac taaggtgaat tgtgaaaacg ctgaaggttg
     1201 aaggttgaag gctgaaggct gaccaagcag ccgggcgttg acttaaaacg gcgcgagcga
     1261 tgagcgagcg ctggcacgcg cgctgggggt ggggccccag cgcaagtgat aaatgcagac
     1321 tgaagacgca atgaaaaacc tcaacatcgg cctgattggc tacgggttca tggggcggac
     1381 gcattcgaat gcgttcctgc aggcgccgcg gttcttcgac ctcgggtgca agccggtgct
     1441 caaggcggtc gcggcgcgga atgcggaccg ggtgaagcag ttcgccgaca actggggcta
     1501 cgaatcctac gacaccgatt ggcggaatct tgttgcccgc aaggatatcg acgtcatcga
     1561 catcgccagc ccgaacgata cgcatcacga gatcgccatt gccgcggcca aggcgggcaa
     1621 gatggtcatg tgcgagaagc cgctcgggcg cacggcgaaa gaagcgaagg agatggtcga
     1681 ggcggtcgaa aaggccggcg tgccgaacac cgtctggtac aactaccgcc gcgttcctgc
     1741 ggtgacgttg atcaagcagt tgctcgacga agggaagttc ggccgcattt ttcactaccg
     1801 tgcgaagttc ctgcaggact ggacgatctc gcaggatctg ccgcagggcg gcgaagggtt
     1861 gtggcggctg gatgtggccg tggccggtag cggcgtgacc ggcgatctcc tcgcccacaa
     1921 catcgatacc gcgctgtggc tgaacggacc gatcaccgaa gtcaccgcga tgaccgaaac
     1981 cttcgtcaag cagcgcaaac acaacttaac cggcaaggtc gagcccgtcg ggatcgacga
     2041 cgccagcgcg tttttgtgcc gcttcgaaaa cggatcgctg gcgatgttcg aagcgacgcg
     2101 gtatgcgcgc gggcacaagg cgctctacac gctcgagatc aacggcgagc acgcgtcggc
     2161 gttctgggat ctgcacgacc tccatcgcat tcagtacttc gatcaccgtg acgaagggcg
     2221 cgtgcgcggc tggcgcagcg tgcacgtgac cgatggcgat catccctaca tgaagcactg
     2281 gtgggtgccg ggcctgcaga tcggctacga gcacacgttc attcatcagt tcgcggattt
     2341 tgccgtcgcg gcaatcaaag ggcagaacat cgcgccgacg tttcgcgatg gtctcgccac
     2401 cgactacgtc accgacgccg tgttgaagtc ggccaagacc ggcaagtggg agaaggtcta
     2461 gcgcctgcgg cccagctact taggacatca aatgcaaaaa aataatgtgt tgctctggcc
     2521 cggcacgtgc gccgcaatcg ttttcttctg cgtcgtggtg ccgggcgcgc aacagcaacc
     2581 gaaccctcct gcgccacagc agccacccgc cggacagccg ccggctggcg cgccgggcgc
     2641 tccagcgcag gggcgcggac gtggcggcgg cgggttccgt cagcccgatc cgatcgactt
     2701 ccaggagcac gaaggatgga cggcgctctt cgacggcaaa acgctgaacg gctggtcggg
     2761 agataacaac tggaaggtcg aggacggtgc catcaccatc gagtcgacat gtgaaaagcc
     2821 gactggcacc atctatctcg tc
//
LOCUS       DATIMH010000809         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1930521, whole genome shotgun sequence.
ACCESSION   DATIMH010000809 DATIMH010000000
VERSION     DATIMH010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930521"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VLI90_14040"
     CDS             complement(<1..478)
                     /locus_tag="VLI90_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15376.1"
                     /translation="MPQGHDNYDQLTNVFASVFSEHPANIALLDLDANLLAVNGAWSR
                     FAEDNGLRRGYEFAGANYLAATEPGAMAGDEYARQAYVGLLGVMRAGLPKFTMVYPCH
                     SPTEQRWFRMWVQPQTPEAAAIVVAHSFLGPTAPSADQEEQETTGEGSTPFELHAGR"
     gene            600..1343
                     /locus_tag="VLI90_14045"
     CDS             600..1343
                     /locus_tag="VLI90_14045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV15377.1"
                     /translation="MSLATQELSVEELYKDYPVARTGGGTPYINLTDDYIAYLSFANA
                     GMLNRGNLLCFDYAIRNLPSNNPIVEIGSFCGLSTNLLAYFKRRRGATNQIFNCDRWE
                     FEGAIGNVGDSGISHSAYRSFVRDSYIRNVRMFSREQLPHTIEQLSDEFFTAWSHRRQ
                     MVDVFGRVAKLGGPISFCYIDGNHEYAAARSDFEHADAFLEPGGFILFDDSADGSAWE
                     VCRVVAEVKQMNHYRLVTKNPNYLFQKIA"
     gene            1394..2095
                     /locus_tag="VLI90_14050"
     CDS             1394..2095
                     /locus_tag="VLI90_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15378.1"
                     /translation="MQRRIYLTLAVLLSISHLAMAIVRVQSAEAAPSKGKSHVVAGEV
                     DEQAAHFKDVDPQTIYDVKLTLDDGVVLQGVNMRWYSIEPERPDAGELSDDDRGQITA
                     VLKDIPAFYNKNDLLILRGNHDRAVALVQLVRDSAFHSDKGGEVIWHVELWYFKNQHG
                     GWEKVQQANRIVRRERFASAKAYHDVIDHLRWVPELGGLKPAEGSTELTVNLPATEPA
                     AQPAGSDTVSTQPAR"
     gene            complement(2022..>2943)
                     /locus_tag="VLI90_14055"
     CDS             complement(2022..>2943)
                     /locus_tag="VLI90_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15379.1"
                     /translation="FPTGFSAITGSVHPFEMTTVQRHLSPSAVPFAATRDLIELYQDR
                     ERFWLIDDRWGMAEINLLKGQWRSWVLPRPTTDALRCCEMAVLWPMAQLLRAKNLHLL
                     PAVSIARSGWAGLIICPFGIEPELSALVRRGYRVIGQRWTALREEDGRIAMLHMPGSV
                     ERAAAPQLRSFGSHDLPGRVDLTAEFLGSHQNHAFCDAVMITEPGRRPAAQLREMDPV
                     DATNVLRHAWPIVDLTPHRRGHEIAPKLAQLCRCGEIQLSRNPKDLLRLLETFRDSRP
                     STRKAPFTAPVELTLYLNQPAAPQVRSLAG"
BASE COUNT          566 a          897 c          947 g          533 t
ORIGIN      
        1 atcggcccgc gtgtagttcg aacggcgtcg aaccttcgcc tgttgtctct tgctcttctt
       61 gatcagccga cggcgcggtt ggcccgagaa aagaatgcgc gacgacgatc gccgccgctt
      121 cgggcgtttg cggttgcacc cacatgcgga accagcgctg ctccgtgggg gaatgacaag
      181 gataaaccat ggtgaacttc ggcaggccgg cgcgcatcac gccgagcagg ccgacgtacg
      241 cctgcctggc gtattcatcg cccgccatcg cgccgggttc ggtggcggcg agataattgg
      301 cgcccgcgaa ttcgtacccg cggcgcaggc cgttgtcctc ggcgaagcgt gaccatgccc
      361 cgttgacggc caagagattc gcatccagat cgagcagcgc gatgttcgcg ggatgctccg
      421 agaagacgct ggcgaagacg ttggtcagtt ggtcgtaatt atcgtggccc tggggcatgt
      481 atgcatcgat ctcattatat gaatcgcccc cgaatgcgat aggtggaatc gctgagcgcg
      541 gctcaccgca attgccgata atagcgcggc tggggcgctt gaaacggaga tcgtcatcga
      601 tgtcgttggc cacacaagaa ctaagcgtcg aggagctcta caaggattac cccgtcgccc
      661 gcacgggcgg gggcacgccg tacatcaacc tcacggacga ctacatcgcc tacctttcgt
      721 tcgccaacgc cgggatgctc aaccgcggca accttctgtg cttcgattac gccatccgca
      781 atttgccctc gaacaacccg atcgtggaga tcggatcgtt ctgcggcctg tcaaccaacc
      841 tgctggctta cttcaagcgc cgccgtggcg cgaccaacca gatcttcaac tgcgaccgct
      901 gggagttcga aggcgcgatc ggcaacgtag gcgattcggg catctcgcac tcggcgtatc
      961 gcagcttcgt gcgcgactcg tacatccgca acgtgcgcat gttcagccgc gagcagctgc
     1021 cgcacacgat cgaacaactg tcggacgaat ttttcacagc ctggtcacac cgccggcaga
     1081 tggtggatgt gttcgggcgc gtcgcgaagc tcggcgggcc gatctcgttc tgttacatcg
     1141 acggcaacca tgagtacgcc gccgcgcgaa gcgattttga acacgccgat gcgttcctcg
     1201 aacccggtgg attcatcttg tttgatgata gcgccgacgg ttctgcctgg gaggtctgcc
     1261 gagtcgttgc ggaagtaaag caaatgaatc attacaggct cgtgacgaag aacccgaact
     1321 acctgtttca gaagatcgcg tgacgcacac gcgcttcggc gcgttcgcac gccgctgatg
     1381 taagatgaga tgcgtgcagc ggcgcatcta tctgacattg gcggttctct tatccatttc
     1441 tcatctggcg atggcgatcg tgcgcgtgca gagcgcggag gccgcgccgt ccaaagggaa
     1501 atcgcacgtt gttgcggggg aagtggatga gcaggccgct catttcaaag acgtcgatcc
     1561 gcagacgatc tacgacgtga agctgacgct ggacgacggc gtggtgctgc aaggcgtgaa
     1621 catgcgctgg tacagcatcg agccggagcg gcccgacgcc ggcgagttga gcgatgacga
     1681 ccgcgggcag atcaccgcgg tgctcaagga catccccgcg ttttacaaca agaacgatct
     1741 gctgatcctg cgcgggaacc acgatcgcgc cgtcgcgctc gtgcagctgg tgcgcgactc
     1801 ggcgtttcat tcagacaaag gcggcgaagt gatctggcac gtcgagctgt ggtacttcaa
     1861 gaaccagcac ggcggatggg agaaggtcca gcaggcgaac cgcatcgtgc ggcgagagcg
     1921 cttcgcatcg gccaaggcat atcacgatgt gatcgatcac ctgcggtggg tgccggaact
     1981 gggcgggctg aaaccggcgg agggttcgac ggagctgacg gtcaacctgc cagcgaccga
     2041 acctgcggcg cagccggctg gttcagatac agtgtcaact caaccggcgc ggtgaacggc
     2101 gccttgcgcg ttgacggccg gctgtcgcgg aaggtttcga gcaatcggag caggtctttc
     2161 ggattgcgcg acagctgaat ctccccgcag cggcagagct gagccaactt cggcgcgatc
     2221 tcatgccctc ggcggtgcgg cgtcaggtcc acgatcggcc aggcatgacg cagcacattt
     2281 gtcgcatcga ccgggtccat ctcgcgcaac tgagcggccg gccggcgacc gggttcggtg
     2341 atcatcacgg catcgcagaa cgcgtggttc tgatgcgagc cgaggaattc cgcggtgaga
     2401 tcgacgcgcc cggggagatc gtgcgagcca aatgatcgca gttgcggggc ggcagcgcgc
     2461 tcgacggagc cgggcatgtg cagcatggca attcggccat cttcttcacg cagcgctgtc
     2521 cagcgctggc cgatcacgcg atagccccgg cgaaccagcg cgctcagttc cggttcgatg
     2581 ccgaacggac agatgatcag tcctgcccac ccgctgcgcg cgatggacac ggccggcagc
     2641 aggtgcaggt tcttcgcccg cagcagctgc gccatcggcc agagcaccgc catctcgcag
     2701 cagcgcagcg catcggtcgt gggccgcggc agcacccagc tgcgccactg gcctttgagc
     2761 aaattgattt ccgccatgcc ccaacggtcg tcgatgagcc agaaccgctc gcggtcctga
     2821 tacagttcga tgaggtcgcg cgtcgccgcg aacggcacgg cactgggcga gaggtgccgc
     2881 tgcaccgtcg tcatctcaaa gggatgaacg ctgccggtga tcgcgctgaa cccggttggg
     2941 aag
//
LOCUS       DATIMH010000810         1589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1639876, whole genome shotgun sequence.
ACCESSION   DATIMH010000810 DATIMH010000000
VERSION     DATIMH010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639876"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="VLI90_14060"
     CDS             complement(<1..1440)
                     /locus_tag="VLI90_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15380.1"
                     /translation="MLSGIAGAALSGTPTNAALPSANPGQAITLTGAGLSTSTGIIGT
                     YTGSDGIVRSVLLRATTASADGTIATLIVPNYFNGVTTLSVLGSSASQTLQIVPTLTS
                     YNVNSVNSLNLYGTGLQEGSATNTVTYNFAGGSVSDTAANSGPDVQNNVSGSDNTFVT
                     LTEPVHGFGTVTTTTAGGTSAPLVLNEFETGLGLIRGIAIDPANPTHLWVADNANPAK
                     IHLIDTTTGQPVTATAQQVNAIPITNVSGATNSLGSTSFFGSIQIIPADFNLSFTPVP
                     AGSLLVFYGPSNPDYVVALDPATGTVISSLQLAGNYDNTAGIYDPSTGELFVVDRNHS
                     GGNRIVAIDPVGGTEDPNFTFAAPFNLGDGGLALDPATSGPNAGTFWLSSDQTGDIVQ
                     VTNTGTVLRHLTVGLQAPGSIGNSGIVFDAAGNLLVSTNQGVVLKLNVNADYATTRPT
                     LTAINATATNGTAANAAIPSADAGQVITLT"
BASE COUNT          234 a          506 c          561 g          288 t
ORIGIN      
        1 ggtcagggtg atcacctggc ccgcgtcggc ggacgggatc gccgcgttcg ccgccgtgcc
       61 gttcgtcgcg gtggcgttga tcgcggtcag cgtcggcctg gtggtcgcgt agtcggcgtt
      121 gacgttcagc ttcagcacga cgccctggtt ggtcgaaacc agcaggttgc ccgccgcgtc
      181 gaacacgatg ccggagttgc cgatgctgcc cggcgcctgc agcccgaccg tcagatggcg
      241 cagcacggtg ccggtgttgg tcacctgcac gatgtcgccg gtctggtccg aactcagcca
      301 gaacgttccg gcgttcggcc ccgatgtcgc gggatccagc gccaggccgc catcgcccag
      361 attgaacggc gcggcgaagg tgaagttcgg atcctcggtg ccgccgaccg gatcgatcgc
      421 cacgatgcgg ttgccgccgc tgtgattgcg gtcgaccacg aacaattcgc cggtcgacgg
      481 atcgtagatc cccgccgtgt tgtcgtaatt gcccgccagt tgcagcgacg agatgaccgt
      541 ccccgtggcc gggtcgagcg ccacgacgta atccgggttg gacggcccgt agaacaccag
      601 caggctgccg gccggcaccg gcgtgaagct gaggttgaag tccgcaggaa tgatctggat
      661 cgagccgaag aacgacgtgc tgccgaggct gttcgtcgcg cccgagacat tggtgatcgg
      721 gatcgcgttc acctgttgcg ccgtcgccgt gaccggctgg ccggtcgtgg tgtcgatcag
      781 atggatcttc gccggattgg cgttgtccgc cacccacaga tgcgtcgggt tcgccggatc
      841 gatggcgatg ccgcggatca ggccgagccc ggtttcgaac tcattcagca ccaacggcgc
      901 cgaggtgccg cccgcggtcg tcgtcgtcac cgtgccgaaa ccatgcaccg gctcggtcaa
      961 ggtgacgaag gtattgtcgg agccgctgac gttgttctgc acgtcggggc cggaattcgc
     1021 cgccgtgtcg ctgaccgatc cgccggcgaa attatacgtg accgtgttgg tggcgctgcc
     1081 ttcctgcagg ccggtgccgt acaggttcag cgagttcacg ctgttgacgt tatagctggt
     1141 cagcgtcggc acgatctgca gcgtttgcga cgccgacgat cccagcacgc tcagcgtggt
     1201 cacgccgttg aaataattcg gcacgatcaa ggtcgcgatc gttccgtccg ccgaggccgt
     1261 cgtcgcgcgc aacagcacgc tgcgcacgat cccgtcgctg ccggtgtagg tcccgatgat
     1321 gccggtcgac gtgctgagcc cagcgccggt gagcgtgatc gcctggccgg gattggccga
     1381 gggcagggcg gcgttggtgg gcgtgccgct gagcgccgcg cccgcgatgc cgctcagcac
     1441 ggtgaccagc ggcgcgctgg tgccgccgtt cgtggtgacg ctgatcggtc cgaaggcgcc
     1501 cgcgctcaac ggcacggtca ggttgaccgt cgtgctggcg ctgaacgagt cgggaccggc
     1561 ggaggtgctg gaatccacga ccgtcgtgg
//
LOCUS       DATIMH010000811         3985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3082603, whole genome shotgun sequence.
ACCESSION   DATIMH010000811 DATIMH010000000
VERSION     DATIMH010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3082603"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VLI90_14065"
     CDS             complement(<1..1162)
                     /locus_tag="VLI90_14065"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HSV15381.1"
                     /translation="MPSLLIQHGTILDPANSIERWSDVLLRDGKIAAIGDNLGKADRV
                     IDAGGKLVTPGLIDIHVHFREPGDEEEETIATGAAAAVAGGFTSVCCMPNTKPPLDTE
                     AAIEFVLRESQRVGLANVYPIGAITKGRAGHELAEIGSMHQRGAIAFSDDGVGVADPS
                     VMRKALQYCKMFDAVIMQHCEEPSLGGGAMHGGIVSTALGLPGIPAEAEQLMIARDLL
                     LNRTIGCRYHVQHISTAWSVELIRRAKRDGQSVTAEVAPHHLLLTDESCRGYDTNYKM
                     NPPLRTAADVQACIDGVRDGTIDCLATDHAPHLAEEKELEFQNAPFGILGLECALPLY
                     AKALVEPGHITWARLIEMMTIAGARIAKLEGKGTLSVGADADMTIIDPNLEWT"
     gene            complement(1209..1628)
                     /locus_tag="VLI90_14070"
     CDS             complement(1209..1628)
                     /locus_tag="VLI90_14070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HSV15382.1"
                     /translation="MDFYEFRGRRDSHVDPVVEDLAEAVIGAAIEVHRELGPGLSELN
                     YRKALCREFDLRGIPHRYEVPVPIMYKGLSVGDGRIDLLVGEKLIIELKAVETISPVH
                     RAQALTYLRVTKLQLALVINFNVSVLKDGIKRVIKTI"
     gene            complement(1681..2652)
                     /locus_tag="VLI90_14075"
     CDS             complement(1681..2652)
                     /locus_tag="VLI90_14075"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HSV15383.1"
                     /translation="MGERDTPASSTDARWTRRHLLSLEELSADEIRFVLDTADSFKEV
                     STRSVKKVPALRGRVMVNAFFEDSTRTRTSFSLAAQRLSADIIDFSGKGSSVSKGETL
                     IDTARNIEAMGVDVIVLRHAAAGSAELLARSVKCSVINAGDGAHEHPTQGLLDIYTIR
                     ERFGRIAGLKVAIVGDIANSRVARSNLWALQKLGAEVILVGPTTLLPKSFEALGARVV
                     HDFDSVIGEVDVINMLRVQNERIKSSQFPTVREFTSLFGLTSERFQKCKPDVFVMHPG
                     PMNRGIEISSHIADGPQSGILTQVTNGLAVRMAVLYLVTQGTAFVSA"
     gene            complement(2738..3280)
                     /gene="pyrR"
                     /locus_tag="VLI90_14080"
     CDS             complement(2738..3280)
                     /gene="pyrR"
                     /locus_tag="VLI90_14080"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HSV15384.1"
                     /translation="MRAHYDGNEVRQMIERLASQIVAAYPPATGPLTIVGIRTRGEVL
                     AERLVPLLHRAGHTNIGRGVLDITLYRDDLSEIGPRPMVRPTRIDVDINARPLILVDD
                     VLFTGRTVRAGLDALADFGRPAAIRLAVLVDRGGRELPIQPDYVSLVERNVPITHRVK
                     VKLAERDGVDEIVIDPRNAG"
     gene            complement(3519..>3985)
                     /locus_tag="VLI90_14085"
     CDS             complement(3519..>3985)
                     /locus_tag="VLI90_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15385.1"
                     /translation="YVKPTGGGKPLAIVGLDDEWSGHIDPKSAWQNVDPGLPIICLNH
                     NPANVKELLPYPWQWMLSGHTHGRQVATSRFGQRFYPHRYRHYTHGYYAVEGRHLYVN
                     RGLSYGQRVLDWCRPEITVFKLEPATNGHVVEMPTPEHRACNPDPVRPGDVK"
BASE COUNT          731 a         1322 c         1169 g          763 t
ORIGIN      
        1 tcgtccactc cagattggga tcgatgatcg tcatatccgc atccgcgccg acgctcagcg
       61 tgcctttgcc ctccagcttc gcgatccgcg cccccgcgat ggtcatcatc tcgatcaacc
      121 tcgcccacgt gatatgcccc ggctcaacga gcgctttggc gtacagcggc agcgcgcact
      181 ccaagccgag aatgccgaat ggcgcgtttt gaaattccag ttccttctct tccgccaggt
      241 gcggcgcgtg atcggtggcg aggcagtcga tcgtgccgtc gcgcacgccg tcgatgcacg
      301 cctgaacgtc cgccgccgtg cgcagcggcg ggttcatctt gtagttcgtg tcgtacccgc
      361 ggcagctctc gtcggtcagc agcagatgat gcggggccac ctccgccgtc accgactgcc
      421 cgtcgcgctt ggcccggcga atcaattcca cgctccacgc ggtgctgatg tgctgcacgt
      481 gataccggca gccgatggtg cgattgagca gcagatcgcg cgcgatcatg agctgttccg
      541 cctcggccgg gatgccgggc aggcccagcg cggtggagac gattccccca tgcatcgccc
      601 cgccgcccag cgacggttct tcgcaatgct gcatgatcac cgcgtcgaac atcttgcagt
      661 actgcagcgc cttgcgcatg accgaggggt cggccacgcc gacaccgtca tcgctgaacg
      721 cgatcgcccc acgctggtgc atcgatccaa tctcagccag ctcatgcccc gcccgcccct
      781 tggtgatcgc gccaatggga taaacattcg ccagccccac gcgctgcgat tcgcgcagca
      841 cgaattcgat cgccgcctcg gtatccagcg gcggcttggt gttcggcatg cagcaaaccg
      901 aagtaaaccc ccccgccacc gccgccgccg cgccggtggc gatggtttct tcctcctcgt
      961 cgccgggctc tcgaaagtgc acgtgaatat caatcaaccc cggcgtgacg agcttacccc
     1021 cggcgtcgat gacgcgatcg gctttgccga ggttatcgcc aatggccgcg atcttgccgt
     1081 cgcgcagcag aacgtcgctc caccgttcga ttgaattggc gggatcgagg atggtgccgt
     1141 gttggatgag tagagaaggc ataatgcgaa cgcgtagccg cgaagaacgc gaaggaagac
     1201 gcgaaggttc agattgtctt tataactcgc ttaatgccgt ccttcaggac cgagacgttg
     1261 aagttgatga cgagggcaag ttgcagcttt gtgacgcgca agtaggtcag ggcttgcgcc
     1321 cgatgcacgg gactgatcgt ctcgacggcc ttcagttcaa tgatcagctt ctcgccgacc
     1381 agcaaatcaa tgcgaccgtc gcccacactc aggcctttgt acatgatcgg gacgggcact
     1441 tcatagcgat gcggaattcc acggaggtcg aattccctgc agagcgcctt acgatagttc
     1501 agctccgaca agcctggtcc cagctcccga tggacttcaa tggcggcgcc gatgaccgcc
     1561 tccgccaagt cttcgaccac ggggtcaacg tgcgagtccc ggcgcccacg aaattcataa
     1621 aagtccacct gttcctcctt cgcgtctgcc ttcgcgccca tcgcgtcttc gcgttcgaat
     1681 tcatgcgctc acgaaggcag tcccctgcgt aaccagatac aacaccgcca ttcgcaccgc
     1741 taagccattc gtcacctgcg tcaaaattcc gctctgcggg ccatccgcga tgtggctgct
     1801 gatttcgatt ccgcggttca tcgggccagg gtgcatcacg aagacatccg gcttgcactt
     1861 ctgaaaacgc tcgctggtta agccgaacag gctggtgaac tcgcgcaccg tgggaaattg
     1921 gctgctttta atgcgctcgt tctgaacgcg gagcatgttg atcacgtcaa cttcgccaat
     1981 gacggagtca aaatcgtgca cgacgcgcgc ccccagtgcc tcgaacgact tgggcagcag
     2041 cgtcgtcggg ccgacgagga tcacctccgc ccccagcttc tgcagcgccc aaaggttcga
     2101 tcgcgccacc cggctgttgg cgatgtcgcc gacgatcgcc accttcaacc ccgcgatccg
     2161 cccaaagcgt tcgcggatgg tgtagatgtc cagcagcccc tgcgtcggat gctcgtgcgc
     2221 gccgtcaccg gcgttgatca cgctgcactt gaccgacctc gccaacaact ccgccgatcc
     2281 cgcggcggcg tggcgcagga cgatcacgtc cacgcccatc gcctcgatgt tgcgggcggt
     2341 gtcgatcagc gtttcgcctt tgctgacgct cgaacccttg ccgctgaagt cgatgatatc
     2401 ggccgagagg cgctgggccg cgagggaaaa gctcgttcgc gtgcgcgtcg aatcctcgaa
     2461 gaacgcgttg accatgaccc ggccgcgtaa cgccggcact tttttcacgc tgcgcgtgga
     2521 aacttctttg aacgagtcgg cggtgtcgag cacgaagcgg atttcgtcgg ccgacagctc
     2581 ctccagcgac agcagatgcc ggcgcgtcca ccgcgcgtca gtcgaagatg ctggcgtatc
     2641 tcgttcaccc acagttcatc gactccgaag ctatatcctt cgaccgcgcg gttgcgcgac
     2701 tgcgcggctg cgccgctacg cttaggataa atgcaagcta tcccgcattc cgcgggtcga
     2761 tcacaatctc atccacgccg tcgcgctcgg ctagcttcac tttcacgcgg tgcgtgatcg
     2821 gcacgtttcg ctcgaccaat gacacataat ctggttggat cggcaattcg cgcccaccgc
     2881 gatcgaccag caccgccagg cgaatcgccg cggggcgacc gaagtcggcc agggcatcga
     2941 gtccggcgcg cacggtgcgg ccggtgaaca gcacgtcatc cacaaggatc agcggtctgg
     3001 cgttgatatc cacgtcgatg cgcgtgggcc gcaccatcgg ccgggggccg atttcggaca
     3061 ggtcatcgcg atagagcgtg atatcgagca cgccgcggcc gatgttcgtg tgcccggcgc
     3121 gatgcagcag cggcacgagc cgctcggcca gcacctcacc gcgcgtgcgg atgccgacga
     3181 tcgtgagcgg gccggtcgcc ggcggatagg ccgcgacgat ttgcgacgcg agccgctcga
     3241 tcatctgccg gacttcgttg ccatcgtaat gcgcccgcat cggcggcgaa ggtatgggaa
     3301 gcctcggggg aattcaattc ggcgggcgga aagcgaacgc gaagacgcga tgtcgcgaag
     3361 gcagacgcta aggaattgca ataaaatgca gttgatcgac tcccacgcta ctcgcgcggg
     3421 cctccggccc gtgctaacgg cgcaaaacat cgaacgatcc actcaatgta aatcttcgcg
     3481 tcttccttcg cggcatcgcg ccttcgcgct cgcctgcgtt acttcacatc gcccggccga
     3541 accggatcag ggttacacgc ccgatgctct ggcgtcggca tttcaacaac gtgtccattt
     3601 gtcgccggct cgagcttgaa caccgtgatc tccggccggc accagtccaa cacgcgctgg
     3661 ccatacgaca acccgcggtt gacgtacagg tgtcggcctt cgacggcata atatccgtgc
     3721 gtgtagtgac gatatcggtg cgggtagaac cgctggccga accggctggt cgccacctgc
     3781 cggccgtgcg tgtggccgct cagcatccac tgccacggat agggcagcag ctccttcacg
     3841 tttgccggat tgtgattgag gcaaatgatc ggcaaccccg gatcgacatt ttgccacgcc
     3901 gacttcggat cgatgtgacc cgaccattca tcatcgagcc cgacaatcgc gagtggcttt
     3961 ccaccgccgg ttggcttcac gtacg
//
LOCUS       DATIMH010000812         1513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_483218, whole genome shotgun sequence.
ACCESSION   DATIMH010000812 DATIMH010000000
VERSION     DATIMH010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483218"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..694
                     /locus_tag="VLI90_14090"
     CDS             158..694
                     /locus_tag="VLI90_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15386.1"
                     /translation="MKLTRSQKVSFAFLAVAVGGFVVDRIFFTPATADAAPPGRSAVA
                     EVVAAGINKIETAVASDVSASVEGLISAKLKTVPGVNVEQVRDVFAPASKWVDAHKPP
                     PAVIARQERSNGVAEFVAGHKLSAVMAGRGGVGGDALVDGQVVHVGQKVGDWRLVSLT
                     SRSAVFTCNGARATLLLP"
     gene            1164..>1513
                     /locus_tag="VLI90_14095"
     CDS             1164..>1513
                     /locus_tag="VLI90_14095"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV15387.1"
                     /translation="MKMSRKHARGFSLIELVIVVVIIAIIGAIAIPKMSRGAQGAGDS
                     ALMQDLSILRSALDLYNAEHPTAALTSSSTSAQVLAALTTYSDALGNANPIKSTTYIY
                     GPYVKPPLPSLPVGS"
BASE COUNT          275 a          439 c          499 g          300 t
ORIGIN      
        1 aacggccagg atgcgccggc gacgtcggcg acgctgacgt tccacctgga cctgcggtgg
       61 tacgcggcgg gggccgcggg ggcggcgggg gcgggggggc attgaactgt gaattccggc
      121 gatgccatcg ccggctttac agggattgga tcgcgatatg aaactcacgc ggagccagaa
      181 agtctcgttc gcgtttctcg ccgtggcggt gggcgggttt gtcgtcgacc ggatattttt
      241 cacaccggcg acggcggacg cggcgccgcc gggacgctcg gccgttgcgg aagttgttgc
      301 cgcgggcatc aacaagatcg aaaccgcggt ggcgagcgat gtcagcgcgt cggtggaagg
      361 gctgatctcc gcgaagctca agacggtccc gggcgtgaac gtcgagcagg tgcgcgatgt
      421 tttcgcgccg gcgagcaagt gggtcgatgc gcacaaaccg ccgccggcgg tgatcgcgcg
      481 gcaggagcgc agcaacggcg tcgccgaatt cgtcgccggc cacaagctgt cggcggtgat
      541 ggccggccgc ggcggggtgg gcggggatgc cctggtggac gggcaggtcg tgcacgtcgg
      601 gcaaaaagtg ggcgactggc ggctggtctc gctaacgagc cggtccgcgg tgttcacgtg
      661 caacggcgct cgggcgacac ttctgcttcc gtgatggatc acctacctga ttcgctgctt
      721 ggcgcgatgc ggcgtttcac tcccgcttgt ccttctacaa ccgcgtttgt cacagagctc
      781 acgccagaag acagaggcta cagagcggaa tcgaatgaaa agtctgccac tgtcatctgg
      841 tgtccctctg tgatcggtgg ttcggccaga tccagagaat ccgcacatcg cgttagtcac
      901 gattcacctt cggcatccgg aaagcagccg tgtcaggtgc gggctcaacg tccgataact
      961 accgctgcgc cgcaggaaaa cgcgtgatct catctcccca caggtgaaga atttcgagat
     1021 gccatgaacc taagatattc ccggcacaga agtcttaaag cttgtggacc ggtgtgacga
     1081 tgaggaagtc tgacagcgga caatttgcag catttcgcat tgtccgtttc gttcgccgac
     1141 cgtttttatt cgagggctcg catatgaaga tgtctcgcaa gcacgctcgg gggttcagcc
     1201 tgattgagtt ggtgatcgtg gtggtgatca ttgcgatcat cggcgcgatc gcgattccca
     1261 agatgagccg tggcgcccag ggcgccggcg actccgcgtt aatgcaggat ctgtcgatcc
     1321 tccgcagcgc acttgacctt tacaacgcgg agcatccgac ggcggcgctc actagtagct
     1381 ccacctccgc gcaggttttg gccgcgctta cgacgtattc cgatgcacta ggcaatgcca
     1441 acccaattaa gtccaccacg tatatctacg gcccttatgt gaagccgccg ctaccgtcgc
     1501 ttccggttgg ctc
//
LOCUS       DATIMH010000813         1928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4818207, whole genome shotgun sequence.
ACCESSION   DATIMH010000813 DATIMH010000000
VERSION     DATIMH010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4818207"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1068
                     /locus_tag="VLI90_14100"
     CDS             235..1068
                     /locus_tag="VLI90_14100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV15388.1"
                     /translation="MSLDSPPAFLAADFIGSTDPRRLQVHRPPGQRDVPYVPTDEPVV
                     AAMMDIAGVGPGDVFYDLGCGDGRLVLAAAARGARAIGVDIDLQRMHECYENRKRTGI
                     RADFVRASFFDVDLHDATVVALYLLPSINVKLRPKLLWEMRPGTRIIANYFSMGDWAA
                     DENRNVCQRLLHKWIVPAWVEGRWRCIVNSPSARQHILLDLHRRYQQVWGTAHVGRRE
                     VPISGAKLAGDELTFAVGRFAFDRPVMRFQCRVSGNQMRGISWEPDRDGPIIKWCGTR
                     R"
     gene            complement(1082..>1928)
                     /locus_tag="VLI90_14105"
     CDS             complement(1082..>1928)
                     /locus_tag="VLI90_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HSV15389.1"
                     /translation="KLILINIPGAPLQAGSVRDHDATVAIIGLVLTAWLIARKIPGAI
                     VVGIIVSTAVAFPLGVTHWPHTTQSISFANAFHADIRGALRWDLLPLLFAVLMVDFFD
                     TLGTATAVGEQAGLVQKSGRVMNIRQILIVDSLAAAIGGLFGASSVTAYVESAAGVAE
                     GARTGLHSIFVGCFFLIAIVAAPYAGVVPAAATAPALILVGFFMIAQITRIDFEDLET
                     AIPAFLTLITIPLTYSIAHGIGYGFVAFVLIKLLSGKPRDVRPLMYAVAAAFVVYFIS
                     EKAHA"
BASE COUNT          396 a          601 c          607 g          324 t
ORIGIN      
        1 gaaaagaaaa gaagctaagc ctgcgtctga aacgaaaaag atgtagttgg gatcacaact
       61 tcttattgtc cgcgatcttg gcgtaaacgc ggttgccctg ggatatccgc ccctttttta
      121 gcacgataat gactggaaaa ggtcaaagca aacaaatttg tctaaaaaat gcacagaata
      181 tcaccgcaga ttgggcattc acgccccgct cggcagttcc ataggctgtc caccatgtcg
      241 ctcgactctc ccccggcgtt tctcgcggcc gatttcatcg gatcgaccga cccccgccgg
      301 ctgcaggtgc accgcccgcc ggggcagcgg gatgtgccat atgtgccgac cgatgagccg
      361 gtggtggcgg cgatgatgga catcgccggc gtcggtcccg gcgatgtgtt ctacgatctg
      421 ggctgcggtg acggccggct cgtgctcgcg gccgcggcac gcggggcgcg cgcgatcggc
      481 gtggacatcg accttcagcg catgcatgaa tgctacgaga accgtaagcg cacgggcatc
      541 cgcgccgatt tcgtgcgcgc cagctttttc gacgtcgatc tgcacgacgc gacggtcgtc
      601 gcgctgtatc tgctaccgag catcaacgta aaacttcgtc ccaagctgtt gtgggaaatg
      661 cggccgggca cgcggatcat cgccaactat ttttccatgg gcgactgggc cgccgacgaa
      721 aatcggaacg tttgccagcg gctgctgcac aagtggatcg tcccggcgtg ggtcgagggg
      781 cggtggcgat gcatcgtcaa cagccccagc gcccggcagc atatactgct cgacctgcac
      841 cgccggtatc agcaggtgtg gggcacggcc cacgtcggcc ggcgcgaggt gccgatcagc
      901 ggcgcaaaac tcgccggcga cgagctgacg ttcgccgtcg gacgattcgc attcgatcgc
      961 cccgtgatgc gcttccaatg ccgcgtcagc ggcaaccaga tgcgcggcat ctcatgggaa
     1021 ccggatcgag acggtccgat catcaaatgg tgtggaacgc gccggtaaag cccgcgcata
     1081 gctatgcgtg ggctttctcg gagatgaaat aaaccacgaa cgccgcggca accgcgtaca
     1141 tcagcgggcg cacatcccgc ggcttcccgc tcaacagctt gatcaatacg aacgcaacga
     1201 acccataccc gatgccgtgc gcgatcgagt acgtcagcgg gatcgtgatc agcgtgagga
     1261 acgccggaat cgccgtctcc aggtcctcga aatcgatccg cgtgatctgc gcgatcatga
     1321 agaagccgac gaggatgagc gccggcgccg tcgccgccgc ggggaccacg ccggcgtacg
     1381 gggcggcgac gattgcgatc aggaaaaagc agccgacgaa gatcgagtgc agtcccgtgc
     1441 gcgccccctc ggccacgccg gcggcggatt cgacgtacgc cgtcacgctg ctggcgccga
     1501 acaacccgcc gatggcggcg gcgagcgaat cgacgatcag gatctggcgg atgttcatca
     1561 cgcggccgct tttctggacg agccccgcct gttcaccaac cgccgtggcg gtgccgagcg
     1621 tgtcgaagaa atcgaccatc aacaccgcga acagcaatgg cagcaggtcc cagcgtaacg
     1681 cgccgcgaat atcagcgtga aacgcgttgg cgaagctgat cgactgcgtc gtgtgcggcc
     1741 aatgcgtcac gcccagagga aacgcgacgg ctgtggaaac gatgatgccg acaacgatgg
     1801 cgccgggaat ttttcgggca atcaaccagg ccgtcagcac cagcccgatg atggcgaccg
     1861 ttgcatcatg atcgcgcacc gaccccgcct gcagcggggc gccggggatg ttgatcagga
     1921 tcagcttc
//
LOCUS       DATIMH010000814         3517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3369176, whole genome shotgun sequence.
ACCESSION   DATIMH010000814 DATIMH010000000
VERSION     DATIMH010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3369176"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VLI90_14110"
     CDS             complement(<1..948)
                     /locus_tag="VLI90_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15390.1"
                     /translation="MLKPFWLVVLSCCLCASPAARADVLKLKDGTSIEGRVIDQGSKY
                     WIKDAAGQSRLINKDDVVSWQKGSTPSAAATPSAPATPSVTPASNIVAGRNFATVKAK
                     ADNCDTPIVAVALWQAFLDNKPAANEVEPAKAELKRWQEMVNGNAEKINGKWIYGEER
                     KKLITHVHQLMKEGNEALIGNQTLVAIDKFEQAVRLYPNNFEANFELGYFNLVKGIDG
                     GRNLAKIDAGIKSLETAVKLRPDSAAALSDLSIGYNFRQKYKLAVQTAYKAAKIEDSK
                     EIVQNLVNSISQAPEGMRSNDPQLKPIMQEATLLAGKYGI"
     gene            complement(1065..1137)
                     /locus_tag="VLI90_14115"
     tRNA            complement(1065..1137)
                     /locus_tag="VLI90_14115"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1102..1104),aa:Lys,seq:ttt)
     gene            1526..2968
                     /locus_tag="VLI90_14120"
     CDS             1526..2968
                     /locus_tag="VLI90_14120"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSV15391.1"
                     /translation="MLVSHRRRVSRAACVESLESRVLYAAGDTFFVSLKGSDANPGDA
                     AHPWRTIQQAMNAATPGSTVNVLPGRYNEKVSVNVSGNATDGFITFQAAGRGVIISGF
                     RKAGPDIIFINNQNYVRILGFDVRDDYNVNNGSGIRITSGGDHIVIQNNRIHLITGIS
                     AMGITAYGSEPTRPISNLVVDHNEIFACFSAPSETLTLNGNVNNFTVTNNFIHNVLGI
                     GIDFISGEGICPDPANDIAHDGVCAGNRVLAAHVRGSRDTAGIWVDGSQNILVEHNIT
                     ELNDVGIEVGCVNPALVTRNITVRSNYVALNRGAGISIGGSDQLAGQVQNCRITNNTV
                     LLNDLSHAGAGEVRLQFASNNTLENNLILAHPGGLLIKSESDAANNASDYNLLFSGLG
                     VRRSPFQFGGTRIAGFSAYQAASGQEQHSIFALPVPLRFVNGTLHITSKSRAVNAGDP
                     AFTGDVGETDIDGDARVLGGRVDIGDDEVA"
     gene            complement(3084..>3517)
                     /locus_tag="VLI90_14125"
     CDS             complement(3084..>3517)
                     /locus_tag="VLI90_14125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV15392.1"
                     /translation="ASGFATGQIRSTTADATHGLGWKDDSANSQVIVAYSLYGDTNLD
                     GAVGVGDLGALATSYGLTTGANWTQGDFNYDGAVNVGDLGALATNYGLSIASGATIGG
                     AATSATIATGSVSVPEPATFGLMCLAAVLGTSRRRTRRQAR"
BASE COUNT          655 a         1132 c         1058 g          672 t
ORIGIN      
        1 gatgccgtac ttcccggcca gcagcgtggc ctcctgcata atgggcttca gctgcgggtc
       61 gttgctgcgc atgccttcag gcgcctgcga aatgctgttg accaggttct ggacgatctc
      121 cttggagtcc tcgatcttgg cggctttgta cgcggtctgc accgccagct tgtacttctg
      181 gcggaagttg taaccgatgg acagatcgct cagcgccgcg gcgctgtccg gccgcagctt
      241 cacggcggtc tccagtgatt tgatccccgc gtcgatcttg gccaggttgc gcccgccgtc
      301 gatgcctttg acgaggttga agtaacccag ctcgaagttc gcttcgaaat tgttcgggta
      361 aagcctgacc gcctgttcga atttatcgat ggcgacgagc gtctgattgc cgatcagcgc
      421 ttcgttgcct tccttcatca gctggtgcac gtgcgtgatg agcttcttgc gctcctcgcc
      481 gtagatccac ttgccgttga tcttctccgc gttgccgttg accatctcct gccaccgctt
      541 cagctccgcc ttggccggct ccacttcgtt cgccgccggc ttattgtcga ggaacgcctg
      601 ccacagcgcc accgcgacga tgggcgtgtc gcagttatcc gccttggctt tcaccgtcgc
      661 gaaattcctg cccgcgacaa tgttggacgc cggcgtcacc gacggggttg caggcgccga
      721 cggagttgcc gccgccgacg gcgtgcttcc cttctgccac gacaccacat cgtccttgtt
      781 gatgagccgc gactgtcccg ccgcgtcttt gatccagtac ttggatcctt gatcgatcac
      841 gcggccttcg atgctggtgc catctttcaa tttcagcaca tcggcccgcg ccgctgggct
      901 cgcgcaaagg cagcaactca agaccaccaa ccaaaacggc ttcaacacgt gggacactcc
      961 tcgaacaaca agaaatggca caatatctgg acggcgcacg ccggatttta acggtggcgc
     1021 cggaggctca caatattggc ggcaaatgca ggagaaggcg gaaatgagcg cgctgggact
     1081 cgaacccagg acctacggat taaaagtccg ttgctctacc aactgagcta cgcgctcgaa
     1141 cggagcggac cgcggtgatt caccgcggcg agcggcggat ggtagtcgag aaccggccct
     1201 gcggcaatcg catcgcagtc gcgcccgccg tccgtgcaga ctctgacgcc gctctcatct
     1261 cagtttcatc aacggaacct atgctcgatc aaccgtgcac ctcgcgaccc tcgtcctgat
     1321 ctacctcggc atcctcgttg ccgaagcgct gcttctcgct tggcacttct ggcgttgtgg
     1381 agtatgggag aggcatcgta aatagccgcg gtggtttttc tttgccctgc gcttgcgcta
     1441 cccgctggtt cccggtcaaa ttgaaacgca gcggacgtgg cgccgctctg ttatctcagg
     1501 gcaatctcac aggattgaga agactatgct tgtttcccat cgccggcgcg tgagccgggc
     1561 cgcgtgcgtt gagtccctcg aatcccgcgt gctctacgcg gcgggcgaca cgttcttcgt
     1621 ctcactcaag ggcagcgacg ccaaccccgg cgatgccgcg cacccgtggc ggacgatcca
     1681 gcaggcgatg aacgccgcca cgccgggatc gacggtcaac gtgttgccgg ggcgatacaa
     1741 cgaaaaggtc agcgtcaacg tctcgggcaa cgcgaccgac ggcttcatca cgttccaagc
     1801 tgccgggcgc ggcgtcatca tcagcgggtt ccgcaaggcg gggccggaca tcatcttcat
     1861 caacaaccag aattacgttc gcatcctcgg cttcgacgtc cgcgacgatt acaacgtcaa
     1921 caacggctcg ggcatccgca tcaccagcgg cggcgatcac atcgtgatcc agaataatcg
     1981 catccacctg atcaccggca tcagcgcgat gggcattacc gcatacgggt cggaacccac
     2041 ccggccgatc tcgaacctgg tcgtcgatca caacgaaatc ttcgcctgct tttccgcgcc
     2101 aagcgaaacg ctgacgctga acgggaacgt caacaacttc accgtcacca acaacttcat
     2161 ccacaatgtg ctgggcatcg gcatcgactt catcagcggt gaaggcatct gcccggatcc
     2221 cgccaacgac atcgcgcacg acggcgtgtg cgccggcaac cgcgtgctgg ccgcccacgt
     2281 gcgcggcagc cgggacaccg cgggcatctg ggtggatggc tcgcagaaca tcctcgtcga
     2341 acacaacatc acggaactga acgacgtggg gatcgaagtc ggctgcgtga acccggcgct
     2401 ggtcacgcgc aacatcaccg tgcgaagcaa ctacgtcgcg ctgaatcgcg gggccggcat
     2461 ttcgatcggt ggatctgatc agcttgcggg ccaggtgcag aactgccgga tcaccaacaa
     2521 cacggtgctg ctgaacgatt tgagccacgc gggcgcgggc gaggttcgcc tgcagttcgc
     2581 gtcgaacaac acgctggaga acaacctgat tctggcgcac cccggcgggc tgctgatcaa
     2641 gtcggagtct gacgccgcga acaacgcatc cgattacaac ctgctgttca gcggcctggg
     2701 ggtgagacga tcgccgttcc agttcggtgg gacgcgcatc gccggcttca gcgcgtatca
     2761 agccgcttcg ggccaagagc agcattcgat cttcgccctg ccggtcccgc tgcggttcgt
     2821 caacggcacg ctgcacatca cgagcaagtc ccgcgcggtg aacgcggggg atccggcgtt
     2881 cacaggcgac gtgggcgaga cggacatcga tggcgacgcg cgcgtgctgg gtggacgcgt
     2941 ggacatcggc gatgacgaag tcgcgtgagg aaggaaagaa atgagtaaca gccgcgggcg
     3001 acagccagcg ggggctttcg cagatcagcc gggctggtgc tcgagcgccc ccgctggctg
     3061 tcgccagcgg ctgttaaaga ttctcaacgg gcctggcgac gcgttcgacg ccgcgatgtc
     3121 cccaacaccg cggcgagaca catcaaaccg aacgtcgctg gctccggaac tgaaactgaa
     3181 ccggtcgcga tggtagcgga tgtcgcggca cccccgattg tcgcgccact ggcgatgctc
     3241 aacccgtagt tcgtcgccag cgcgcccagg tcgccgacat tgacggcgcc gtcgtaattg
     3301 aaatcgccct gcgtccagtt ggcgccggtg gtgaggccgt agctggttgc caatgccccg
     3361 aggtcgccga cgcctacggc gccgtcgaga ttcgtgtcgc catagagcga ataggcgacg
     3421 atcacctgag agttggccga atcatctttc cagccaaggc cgtgcgttgc gtcggcggtg
     3481 gtgctgcgaa tttgtcccgt ggcaaaaccg ctcgcat
//
LOCUS       DATIMH010000815         3258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5682522, whole genome shotgun sequence.
ACCESSION   DATIMH010000815 DATIMH010000000
VERSION     DATIMH010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5682522"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VLI90_14130"
     CDS             <1..373
                     /locus_tag="VLI90_14130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HSV15393.1"
                     /translation="PEDAYVFVQSGLNYTVERVHKNTEPDASHHVGGRELCEGLRELA
                     LKQWGMLARTVLQRWNITSTMDFGRIVFAMVQHDMLQKTDEDTIEDFRQVYDFRTAFE
                     SDYRIEPKIVSERPKRAERQS"
     gene            430..555
                     /locus_tag="VLI90_14135"
     CDS             430..555
                     /locus_tag="VLI90_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15394.1"
                     /translation="MTSILFALWVVAMLVMYFSTVYPERHPNQKAALSATTKADG"
     gene            complement(608..1228)
                     /locus_tag="VLI90_14140"
     CDS             complement(608..1228)
                     /locus_tag="VLI90_14140"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HSV15395.1"
                     /translation="MKRWLMMFLEVGLIVLLAIWVVHAMKASPAKRRDSVEVSIQLPD
                     AKEKATLAPGDDGRYRYTILQTDGQTVELTPDELAARLHREQASRTWIQKVLNVNNPF
                     GYLWVGLGLLGQALFTGRMVVQWLVSEKAKKSTVPPIFWWMSLVGSTMLMAYFLWRRD
                     PVGLLGQSFGWFIYIRNLLFIYRPHAPHTDSIMEAEATEGAAVVTK"
     gene            complement(1232..1837)
                     /locus_tag="VLI90_14145"
     CDS             complement(1232..1837)
                     /locus_tag="VLI90_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15396.1"
                     /translation="MGIYLWLVLKNVIGWALILASGPVGLTVPGPGGLPLFIIGFAMI
                     TFPGKRALTSRVLRGRPWRQQSARTIVVEILVALLLPALVVWVLSLRFRDELQSYGPA
                     AVFTVYALAVVASFMLVRLGLRLLNYGVTFVPALRRKIRPWLRHHGFDLLPPRRRKRL
                     QHHRLRDVNSIRGDEIDTGILEFSPRFHDRLRSLWRRFRIG"
     gene            complement(2237..>3258)
                     /locus_tag="VLI90_14150"
     CDS             complement(2237..>3258)
                     /locus_tag="VLI90_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV15397.1"
                     /translation="KPKAVLVGCGKIAGTWLDAVSKTPELELVALVDLHCPAAEYWAG
                     KFNLPASMIYLAMDAALAATGAELVFDLTVPTAHESVVTAALKSGRHVLGEKPMSETL
                     DSARRMIAAANAAGRTYAVMQNYRFNPEIRALHRFLGSDQIGPVEEVHVDFFIGGHFG
                     GFRDEMAFPLLLDMSIHTFDMARFLTGADPVNVFCYSFNPKRSWYRGDASAIVIFELT
                     GGIVFTYRASWCSEGQITPWNGQWLLVGSKGTVRWDGASTFKAQRVKPDAKPDFIMEQ
                     QSLDVPVTPMEATGHAGCMREFLSAVREGRPPETDCRDNIKSLAMVLAAVESAKARAK
                     VGVTW"
BASE COUNT          638 a         1097 c          958 g          565 t
ORIGIN      
        1 tcctgaagac gcgtatgtct ttgtccagtc aggccttaac tacaccgtcg aacgcgttca
       61 caagaatacc gagcccgatg ccagccatca cgtcggtggg cgcgagctgt gcgaaggcct
      121 gcgcgagctg gccctgaagc aatggggcat gctcgcccgc accgtgctgc agcgctggaa
      181 catcacctcg acgatggatt ttggccggat cgttttcgcg atggtccagc acgacatgct
      241 gcagaagacc gacgaggaca cgatcgagga tttccgccag gtatacgact tccgcaccgc
      301 gttcgaatcg gattaccgca tcgaaccgaa gatcgtgagc gaacgaccca agcgcgcgga
      361 acggcagtcg tgagcgagcc ggcttctccg tcaccctccc ctctcaaacc ccgccgcggg
      421 ttgttaattg tgacgagtat tcttttcgcg ctctgggtgg tcgcgatgct ggtgatgtat
      481 ttctcgacgg tctatcccga gcggcatccg aatcagaaag cagcgctgag cgcgacgacg
      541 aaagccgatg gatagctatc cgtaggtttt gaattcttaa ggggcgcccg cgcgcgatcg
      601 cgcgtgttca cttcgtcacc accgccgcac cttcggtggc ttccgcttcc atgatcgagt
      661 ccgtgtgcgg cgcgtgcggc cgatagataa acagcaaatt ccgaatgtaa ataaaccacc
      721 cgaacgactg ccccagcaac cccaccggat cgcgccgcca gaggaagtac gccatcagca
      781 tcgtcgaacc gacaaggctc atccaccaga agatcggcgg caccgtggac ttcttcgcct
      841 tctcgctgac cagccactgc acgaccatcc gcccggtgaa cagcgcctgc ccgagcaggc
      901 ccaggccgac ccacaggtag ccgaacgggt tgttcacatt cagcaccttt tgaatccacg
      961 tccgcgacgc ctgttcgcgg tgcaatcgtg ccgccaattc atctggcgtc agctcgaccg
     1021 tctgcccgtc ggtctgcaaa atcgtgtagc gatatcgccc gtcatcaccg ggcgcgagcg
     1081 tcgctttctc cttcgcgtcc ggcagctgga tcgacacctc cacgctgtcg cggcgcttgg
     1141 ccggactggc cttcatcgcg tgcacgaccc agatcgccag caggacgatc aggccgacct
     1201 ccagaaacat catgagccat cgcttcatgt cctacccaat ccgaaagcgc cgccagagcg
     1261 accgaagccg atcgtggaag cgcgggctaa attcgagaat gccggtgtcg atttcatcac
     1321 cgcgtatgga attgacgtcg cgcaggcggt gatgttgcag ccgtttccgc cgccgcgggg
     1381 gcagcaggtc gaacccgtga tgccgcaacc acggtcggat cttgcggcga agcgcgggca
     1441 caaatgtcac gccgtagttc aacaggcgca ggcccagccg cacgagcatg aagctggcga
     1501 cgacggccag cgcgtagaca gtgaacaccg ccgccgggcc atacgattgc agctcatcgc
     1561 gaaaccgcag cgacaacacc cacacgacca gcgccggcaa cagcagcgcg acgagaatct
     1621 cgaccacgat cgttcgcgcc gactgctgcc gccacggccg gccgcgcagc acgcgactgg
     1681 tcagcgcgcg cttgccgggg aacgtgatca tcgcgaagcc gatgatgaac agcggcaacc
     1741 cgcccggccc ggggaccgtg agcccgaccg gcccggaggc gaggatcagc gcccacccga
     1801 tgacgttttt gaggacaagc caaaggtaaa tacccacgcg ccgcccccgc gggggtgaac
     1861 ttaacacggg ttggggcgac gaaggcatca gcaccgaacg tctgattgta gtgcggcgcc
     1921 gcgcgaggat cgagaaaccc cgccgcttgc ggcttcgcaa cccacgccac cggcggcctg
     1981 cgcaatcgcg tgcgaccgag cgttctttca ctcgtggcta tgtaagtgcg accaactaaa
     2041 tccgaatcgt catggagacg ttgaaagggg tctcccttcc accccgggtt cggagcgtac
     2101 tcgcgacgaa gcgagatgct tctgtgggag tagaaaaaga atctcgcttc gtcggcgtac
     2161 ctccgaaccc ggcgtgggat tcattccttc gtcgactccg gcgatcacgc ctgagctgac
     2221 gatgaccagc gaagaatcac cacgtcaccc cgaccttcgc acgcgccttc gcgctctcca
     2281 ccgccgccag caccatcgcc aggctcttga tattgtcccg gcaatccgtc tccggcggtc
     2341 ggccttcgcg caccgctgac aagaactccc gcatgcaccc cgcgtggccg gtcgcctcca
     2401 tcggcgtcac cggcacgtcc agcgattgtt gttccatgat gaaatctggc ttggcgtccg
     2461 gcttcacgcg ctgcgctttg aacgtgctcg cgccatccca gcgcaccgtg cccttggagc
     2521 cgacgaggag ccactgcccg ttccacggcg tgatctggcc ttcgctgcac cacgaggcgc
     2581 gatacgtgaa gacgatgccg ccggtcaact cgaagatgac gatcgccgac gcgtcgccgc
     2641 ggtaccacga gcgcttgggg ttgaacgaat agcagaagac gttgacgggg tcggccccgg
     2701 tgaggaagcg ggccatgtcg aaggtgtgga tcgacatgtc cagcagcagc ggaaacgcca
     2761 tctcatcgcg gaagccgccg aagtgcccgc cgatgaagaa gtcaacgtgc acctcttcga
     2821 ccgggccgat ctggtcgctg cccagaaagc gatgcagcgc gcgaatctcc gggttgaagc
     2881 ggtaattctg catcaccgcg tacgttcgcc ccgcggcgtt tgccgcggcg atcattcgcc
     2941 gtgcgctgtc gagcgtctcg ctcatcggct tctcgcccag cacgtgccgg ccagatttta
     3001 gcgccgcggt gacgaccgat tcatgcgcgg tcggcaccgt caggtcgaag acgagttccg
     3061 cccccgtcgc ggcgagggcg gcgtccatcg ccagataaat catgctcgcc ggcaggttga
     3121 acttccccgc ccaatactcc gccgccgggc aatgcaaatc gaccagcgcg accagctcca
     3181 attccggcgt cttgctcacg gcatcgagcc aggttccggc gattttccca cagccgacca
     3241 gcaccgcttt tggttttt
//
LOCUS       DATIMH010000816         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4235963, whole genome shotgun sequence.
ACCESSION   DATIMH010000816 DATIMH010000000
VERSION     DATIMH010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4235963"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1540)
                     /gene="atpA"
                     /locus_tag="VLI90_14155"
     CDS             complement(29..1540)
                     /gene="atpA"
                     /locus_tag="VLI90_14155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSV15398.1"
                     /translation="MAINVAEITSVLKREIAGFEQQLQVSEVGTVIEVGDGIARIYGL
                     RNAMAGELLEFQSGVMGQVFNLEEDSIGSVVFGDYLSIKEGDTVKSTGRLLEVPVGEA
                     VIGRVVNPLGEPLDGAGPIASNETRKLDIIAPGIADRKPVSEPLQTGIKAVDSMIPIG
                     RGQRELIIGDRKTGKTAIAIDTIINQKGKGVKCFYVAIGSKESTVAGIVEVLKAAGAM
                     DYTTVVVASAADPAPLQYIAPYAGCTMAEYFMWKGEATLCVYDDLSKQAAAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAVKLSDEKGGGSLTALPIIETQEGEVSAYIPTNV
                     ISITDGQIYLEPDLFFAGVRPAINVGISVSRVGGNAQTKGMKKVAGSLKLDLAAYREL
                     EAFAQLGTELDKATQAQLDRGARLVELLKQPQYKPMPVEQEVMVIYAGTQGYLDDVPI
                     ARVQEFQTGLLQYIDASYRTLRDQLAEKKELSSDLETQLKDALKTFKASVWKK"
BASE COUNT          249 a          510 c          487 g          338 t
ORIGIN      
        1 taataacaag cttttgcgcg aatccgtgct acttcttcca gacagacgcc ttgaacgtct
       61 tcagggcgtc cttcaactgc gtttccaggt ccgagctcaa ttccttcttc tcggccaact
      121 ggtctcgaag ggtccggtac gaggcgtcga tgtactggag caacccggtc tggaactcct
      181 gcacgcgggc gatcgggacg tcgtccagat acccctgcgt gcccgcgtaa atcaccatga
      241 cttcctgctc caccggcatc ggcttgtact gcggctgctt cagcaactcg acgaggcgtg
      301 cgccgcgatc gagctgcgct tgcgtggcct tgtccagttc ggtcccgagc tgggcgaacg
      361 cttccagctc gcggtatgcc gcgagatcga gcttcagcga gcccgcaacc ttcttcatcc
      421 ccttggtctg ggcgttgccg cccacgcggc tcacgctgat gccgacgttg atcgcggggc
      481 ggacgccggc gaagaacagg tcgggctcga gatagatctg cccgtcggtg atgctgatga
      541 cgttggtcgg gatgtacgcc gacacttcgc cttcctgcgt ttcgatgatc ggcagcgcgg
      601 tcaagctgcc gccgcccttt tcgtcgctga gcttcacggc gcgctcgagc aaccggctgt
      661 ggagatagaa cacgtcaccc ggatacgcct cgcgacccgg cgggcgacgc agcagcagcg
      721 acagttgccg atacgccgcc gcctgcttcg acagatcgtc gtacacgcac agcgtggctt
      781 cgcccttcca catgaagtac tcggccatcg tgcaacctgc atatggcgcg atgtactgca
      841 gcggggcggg gtcggcggcg ctggcgacga ccaccgtcgt gtagtccatc gcgccggcgg
      901 ccttcagcac ttcgacgatc ccagcgacgg tcgattcctt cgaaccgatc gcgacgtaga
      961 agcactttac gcccttgcct ttttgattga tgatcgtgtc gatcgcgatg gcggtcttgc
     1021 cggtcttgcg gtcgccgatg atcagctcgc gctgcccgcg gccgatgggg atcatgctgt
     1081 ccaccgcctt gatgccggtc tgcagcggtt cgctcacggg ctttcggtcg gcgatgccgg
     1141 gcgcgatgat gtcgagcttg cgcgtctcgt tcgacgcgat cggcccggcg ccgtccagcg
     1201 gttcacccag cgggttcacc acgcggccga tgacggcttc gccgaccggc acttccagca
     1261 ggcgcccggt ggacttgacg gtgtcgcctt ccttgatgga caggtagtcg ccgaagacga
     1321 ccgaaccgat cgagtcttct tcgaggttga acacctggcc catgacgccg ctctggaatt
     1381 cgagcagttc accggccatc gcattgcgaa ggccgtagat gcgggcgatg ccgtcaccga
     1441 cttcgatgac ggtgccgact tcgctgacct gcagctgctg ctcgaagccg gcgatttcgc
     1501 gcttgaggac actggtgatt tccgcgacgt tgatggccat cgtttattct ccgattttct
     1561 gtagggagct tttcgttcgc agag
//
LOCUS       DATIMH010000817         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4819391, whole genome shotgun sequence.
ACCESSION   DATIMH010000817 DATIMH010000000
VERSION     DATIMH010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4819391"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VLI90_14160"
     CDS             complement(<1..1294)
                     /locus_tag="VLI90_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HSV15399.1"
                     /translation="MHAARSQRISTAARTKASPRAGAQRGDPTAAGERDRLKHVALIM
                     DYAMSPRRRLIRGVARYVQEHEPWAIYLTPAGVEKSLTSWLRNWNGDGVIVYANDPDA
                     AALLQSKLPVVDLFGKLVSDNVPLVHADDQAIGRLGAEHLLERGFHQFGFWEYTHPDT
                     EWSIQRRRGFEETIRNAGFTCDVYRSAFPKPGSGGPETWERQQHDLTAWLTRLPKPAG
                     VMTSTDLSGQQMLEACLRARVHVPEEIAVVGADNDELVCRIAIPPLSSVIINDEQRGY
                     EAAALLDRLMVGEPAPKRPVLIEPVGVVTRASTDIMAIDDPAVAQAMRYLREHASEDI
                     GIDDVARAVAVSRTVLVRRFRRLVGRSIHQEIIRQRLNHAIQLLGDTQLELKVIARKA
                     GFGSQAYMNAVFQEKLGKTPGSFRNHLRPATIETGATED"
     gene            1720..>2953
                     /locus_tag="VLI90_14165"
     CDS             1720..>2953
                     /locus_tag="VLI90_14165"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HSV15400.1"
                     /translation="MNSPLPRELLQKMDAYWRAANYLSVGQIYLLANPLLRQPLKIEH
                     IKPRLLGHWGTTPGLNFVYLHLNRLIKKQDLDMIYVAGTGHGGPGVVANTYLEGTYSE
                     VYPNISQDEAGMQRLFKQFSFPGGIPSHVAPETPGSINEGGELGYSLTHAYGAAFDNP
                     NLIVACVVGDGEAETGPLAASWHSNKFLDPGRDGAVLPILHLNGYKINNPTLLARIPH
                     EELASLFRGYGYEPHFVEGDEPEAMHQLMAGTLDKIIAEIRQIQASARQSGFKQRPRW
                     PMIVLRSPKGWTGPKIVDGKPAEGSWRSHQVPLSEMAKNPAHVKQLGKWMRSYRPEEL
                     FDKNGALMPELRELPPRGERRMSANPHANGGLLLRDLRLPDFTDYAVDVPSPGAVAGE
                     ATRVLGQFLRDVMKQNRDS"
BASE COUNT          542 a          929 c          936 g          546 t
ORIGIN      
        1 catcttccgt cgctcccgtt tcaatggtgg ccgggcgaag gtggttgcgg aatgagcccg
       61 gggtcttgcc cagcttctcc tgaaacaccg cgttcatgta cgcctgcgat ccaaatccgg
      121 cttttctcgc gatcacctta agctccagct gcgtgtcgcc gagcagttgg atcgcgtggt
      181 tcagccgctg gcggatgatt tcctggtgga tcgatcgacc gaccagtcgg cggaagcgcc
      241 ggaccagcac ggtgcgcgag acggccaccg cgcgggcgac gtcgtcgatg ccgatgtctt
      301 ccgacgcgtg ctcgcgcaga tatcgcatcg cctgggcgac ggccggatca tcgatggcca
      361 tgatgtcggt tgacgcgcgc gtgacgacgc cgaccggttc aatcagcacc gggcgcttcg
      421 gcgctggctc gccgaccatc aaccggtcga gcaaggcggc cgcttcgtag ccgcgctgtt
      481 cgtcgttgat gatcacgctc gacagcggtg gaatcgcgat tcggcagacc aattcgtcgt
      541 tgtccgcgcc gacgaccgcg atctcttccg gcacgtgcac gcgcgcccgc aaacacgctt
      601 cgagcatctg ctggcccgac aggtccgtcg atgtcatcac gccggccggt ttgggcaggc
      661 gcgtcaacca cgccgtcagg tcgtgctgtt ggcgctccca ggtttcgggc ccgccggaac
      721 caggcttggg aaacgccgaa cgatagacat cgcaggtgaa gccggcgttg cgaatggtct
      781 cctcaaatcc gcggcggcgt tggattgacc actcggtgtc gggatgcgtg tattcccaaa
      841 agccgaactg atggaagccg cgctccagca gatgttcggc gccaagtcgg ccgatggcct
      901 gatcgtccgc gtgcaccagc gggacgttgt cggacaccag cttgccgaac agatccacca
      961 ccggcagctt gctctgcagc agggcggcag catcggggtc gttggcgtaa acgatcacgc
     1021 cgtcgccgtt ccaattccgc agccagctgg tcagcgactt ctccacgccc gcgggcgtca
     1081 ggtagatggc ccacggctcg tgctcctgga cgtatcgcgc cacaccgcgg atcaaccgcc
     1141 gccgcggcga catcgcgtag tccatgatca gcgcgacgtg cttcaaccga tcgcgctcgc
     1201 ccgccgcggt gggatcgccg cgttgcgcgc cggcgcgcgg cgacgctttc gtacgtgcgg
     1261 cggtgctgat cctctggctt ctggcagcgt gcatagcgat cttccaaggc ggcaacttca
     1321 cggcgcgagc taaaagccga atccatttgt cgagttcgag acgcgcccgc ccgtgatccc
     1381 cattttagcg gcgagcatgg caatcactat aaccgttgcg cgagtgcaaa gacgcagtcg
     1441 aaagaaatgc aaaagaattg aagcgcggag ccgcagaggc gcagagtcgc ggagacgcgg
     1501 agacgtgctc gaacatctgg cacatctctg cgactctgcg catcggccat aaaaattcga
     1561 ttctctcttg cttagcgtct cggcaccgcg aaatcttgtg aatccacctt gcggtggcgc
     1621 gaagggttgt ggcatccttc ccgccttcca atcgcgtgat tcgcgcacga ccgcccgggt
     1681 cgagatgctc tgccggtggg cagaagaaag tgggctgaca tgaactcgcc gctgcctcgc
     1741 gaactgctgc agaagatgga cgcttattgg cgtgccgcca attacctgtc cgttgggcag
     1801 atctatttgc tggctaatcc gctgctgcgg cagccgctga agatcgagca catcaaaccg
     1861 cggctgctcg gccactgggg caccacgccc ggtttgaact tcgtctacct gcacctcaat
     1921 cgcctgatca agaagcagga cctggacatg atttatgtcg ccggcacggg gcacggcggg
     1981 ccgggcgtcg tcgcgaatac ttatctggag ggaacgtatt cggaggtcta tccgaacatc
     2041 agccaggacg aagcggggat gcagcggctg ttcaagcagt tctcgtttcc cggcggcatt
     2101 cccagtcacg tcgcgcccga aacgccgggg tcgatcaatg aaggcggtga gttgggctac
     2161 tcgttaacgc acgcctatgg tgcggcgttc gataatccca acctcattgt cgcctgcgtc
     2221 gtcggtgacg gcgaagccga gaccggcccg ctggccgcca gctggcattc gaacaagttc
     2281 ctcgatcccg ggcgcgacgg agcggtgctg ccgatcctgc atctcaacgg ctataagatc
     2341 aacaatccca cgctgctggc gcgcattccg cacgaggaac tagcgtcgct gttccgcggc
     2401 tatggatacg agccgcactt cgtcgaaggc gatgagcccg aagcgatgca ccagctgatg
     2461 gcggggacgc tggacaagat cattgctgag atccgacaga ttcaagcgag cgcacggcag
     2521 agtgggttca agcagcgtcc gcgctggccg atgatcgtgc ttcgctcgcc caagggctgg
     2581 acgggtccga agatcgtcga tggaaagccc gccgaaggtt cgtggcgatc gcaccaggtg
     2641 ccgctgtcgg agatggccaa gaatccagct cacgtgaagc agctggggaa atggatgcgc
     2701 agctatcgtc ccgaagaatt gttcgacaag aacggcgcgc taatgcctga gctgcgggaa
     2761 ctgccgccgc ggggcgagcg gcggatgagc gccaatcccc acgccaacgg tggtctgctc
     2821 ctgcgagatc ttcgcttgcc ggacttcacc gactacgccg ttgatgtccc gtcgcccggc
     2881 gccgtcgcgg gggaagcgac ccgcgtcctc gggcaattcc ttcgcgatgt catgaagcag
     2941 aaccgcgatt cgg
//
LOCUS       DATIMH010000818         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4819769, whole genome shotgun sequence.
ACCESSION   DATIMH010000818 DATIMH010000000
VERSION     DATIMH010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4819769"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1622
                     /locus_tag="VLI90_14170"
     CDS             189..1622
                     /locus_tag="VLI90_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSV15401.1"
                     /translation="MATTQSGSSRPIEIETLEPRVLYAATYFVSIKGNDANAGDAAHP
                     WRTIQQAMNAATPGSTVNVLPGRYNEKVSVNVSGNATDGYITFQATGPNVVISGLRKA
                     GANVIFINNQNYVRILGFDIREDYNVIDGSGIRVTSGGDHIVIQNNRIHGITGSSAMG
                     ITAYGSEPARPISNLIIDHNEIFSCWSAPSETLTLNGNVNNFAVTNNYIHNVTGIGID
                     FISGEGICPDPANDITHDGVCSGNKVIAARFRGGRDTAGIWVDGAQNLVIEHNISQQN
                     DVGIEVGCVNPALVAQNVTVRDNYVAMNSQCGISIGGSDAFAGRVQNCQVTNNTLFNN
                     AMSRDSDGEIRLQYASNNTIESNLMLTRPGGYLIKSEVGASNNVSDYNLMFTATGAKR
                     ALFEFGGTPISGVDAYRAASGQDQHSIFASPGALRFANGVAHVTAKSAAVNAGDPLFT
                     GAAGETDIDGEARVLGGRVDIGADEVA"
     gene            complement(1651..2649)
                     /locus_tag="VLI90_14175"
     CDS             complement(1651..2649)
                     /locus_tag="VLI90_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15402.1"
                     /translation="MMDTVLDQFLRDGGHHPVLIDVGASGASPGLWQPIAKHAVHVAF
                     DPDLREMKQQSDGQWYRSTILNEAITPDPNSNEVRFNLTRSPYCSSVLTPDKTLVSNF
                     FEAERFDVLQQTTARATTLDNVLKRLSLDHIDWLKLDTQGTDLRLYNSISPDVRATML
                     AVDLEPGLRGAYVGEDLFGDVHRDLRRDGFWLANLRLDGFVRMRSATLESIGKAAPDL
                     TRAEIEKAVRKVPGWVEVRYLRSLEWMASRQASQRDYQLLWLFAMLDGQFGFALDVCL
                     QYEQTFRPDKASAIMGSEPVQRIRSTRAAQRQKDRVSLPRRVYRKLRGIAKKVLKS"
     gene            complement(2721..3323)
                     /locus_tag="VLI90_14180"
     CDS             complement(2721..3323)
                     /locus_tag="VLI90_14180"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HSV15403.1"
                     /translation="MSAKLTNALSIVSTFAVLALAAPVASAHPGHSSDLPWISGVLHP
                     FTGIDHLLAMIAVGLLGAQMGRRWIWSMPATFVAFMIAGGVLAMTGLAMPLVEPGIMA
                     SVLVLGLMVARAGRLPAGAPVALVGLFAMLHGYAHVSERPLNASLLTYSIGFISSTLA
                     LITTGLAIGLAAKRLQLAQVLRLGGAAIAASGVLLMAHVL"
BASE COUNT          685 a         1128 c         1145 g          596 t
ORIGIN      
        1 tgaccagggt gtgcgctgac gcactcccgc gcattcccct gccgggatgt tccgataacc
       61 ctgttggata agccgcatat cccgaactca acgcgcaggc ggcgcagccg gcgctgtttt
      121 gatctggcgt ctctcactca acccgcccgt tcagtcgccg cggtcgcgtt ctcgggatac
      181 acatgactat ggcgactacg caaagcggtt cctcccgtcc catcgaaatc gaaacgctcg
      241 aaccccgcgt gttgtacgcg gccacttact tcgtctcgat caagggcaac gacgccaacg
      301 cgggggacgc ggctcatccg tggcggacga ttcagcaggc gatgaacgcg gcgacgccgg
      361 ggtccaccgt caacgtgctg ccggggcggt ataacgagaa ggtcagcgtc aacgtctcgg
      421 gcaacgcgac cgatgggtac atcacgttcc aggccactgg gccaaacgtc gtgatcagcg
      481 ggctgcgcaa ggccggcgcc aacgtcatct tcattaacaa ccagaattac gttcgcatcc
      541 tcggctttga tattcgcgag gactacaacg tcatcgacgg cagcggcatt cgcgtgacca
      601 gcggcggcga tcacatcgtc atccagaaca accgcatcca cggcatcacc ggcagcagcg
      661 caatgggcat caccgcgtat ggctccgagc cggctcggcc gatctcgaac ctcatcatcg
      721 atcacaacga gattttcagc tgctggtccg cgcccagcga aacgttgacg ctcaacggca
      781 acgtcaacaa ctttgccgtc accaataact acatccacaa cgtcacgggc atcggaatcg
      841 acttcatcag cggggaaggc atctgccccg acccggcgaa cgacatcacg cacgatggcg
      901 tgtgcagcgg gaacaaggtg atcgccgccc gcttccgcgg cgggcgcgat accgccggca
      961 tctgggtgga cggcgcgcag aatctggtga tcgagcacaa catctcgcag cagaacgacg
     1021 tcggcatcga agtcggctgc gtcaacccgg cgctggtcgc gcagaacgtg acggtgcggg
     1081 acaactacgt ggcgatgaac tcgcaatgcg gcatttcgat cggcgggtcc gatgcctttg
     1141 ccggccgggt gcagaattgc caggtcacga acaatacgct gttcaacaac gcgatgagcc
     1201 gggacagcga cggcgagatt cgcctgcaat atgcgtcgaa caacacgatc gagagcaacc
     1261 tgatgctgac ccggccgggt ggttatttga tcaagtcgga ggtcggtgcg agcaacaacg
     1321 tgtcggacta caacctgatg ttcacggcaa cgggtgccaa gcgggcgctg ttcgagttcg
     1381 gcggcacgcc gatcagcggg gtggatgcgt atcgagccgc gtcggggcag gaccagcatt
     1441 cgatcttcgc atcgcccgga gcgctccgct tcgccaacgg tgtcgcgcat gtcaccgcga
     1501 aatccgcggc cgtgaacgcg ggcgacccgc tgttcaccgg agcggcgggg gagacggata
     1561 tcgacgggga agcgcgcgtg ctgggtggtc gcgtggatat cggcgcggat gaagtggcgt
     1621 gatcagacac cggcgtcggt tgcgcgctcg ctacgacttc agcaccttct tcgcgatccc
     1681 gcgcagcttg cgatagacgc ggcggggcaa ggagacgcgg tccttctggc gctgcgccgc
     1741 ccgggtggag cggatgcgct gcaccggttc gctgcccatg atggcggagg ccttgtcggg
     1801 gcggaaggtt tgctcgtact gcaggcacac gtccagggcg aagccgaatt gaccgtcgag
     1861 catcgcgaag agccagagca gctggtagtc gcgctggctt gcttgccgcg aggccatcca
     1921 ctccagcgat cgcaggtatc gcacttccac ccaccccggc actttccgca ccgccttttc
     1981 gatttctgct cgcgtcaaat ctggtgcggc tttgccgatc gattccagcg tggccgagcg
     2041 catgcgcacg aagccgtcga ggcgcaggtt ggcgagccag aaaccatcgc ggcggaggtc
     2101 gcggtggacg tcgccgaaga ggtcttcacc gacgtacgcg ccgcgcaggc cgggctcgag
     2161 atcgacagcc agcatggtcg cgcgcacgtc gggcgagatg ctgttgtaca gccgaaggtc
     2221 cgtgccctgc gtgtcgagct tgagccagtc gatgtgatcg agcgacagcc gtttcagcac
     2281 gttgtccagc gtcgtcgccc gcgcggtcgt ctgctgcaac acgtcgaagc gctccgcttc
     2341 gaaaaagttc gacaccagcg tcttgtccgg cgtcagcacg ctggagcagt agggcgatcg
     2401 cgtcaagttg aagcggactt cgttgctgtt cggatcaggc gtgatcgctt cgttcaggat
     2461 cgtcgagcgg taccattggc catcgctctg ctgcttcatc tcgcgcaggt ccgggtcgaa
     2521 tgccacgtgc accgcgtgct tggcgatcgg ctgccaaagc cccggcgatg cgcctgaagc
     2581 gccgacatcg ataagcaccg gatgatgccc gccatcgcgc agaaactgat ccagcaccgt
     2641 gtccatcatc gtggtctccg tgcggcttcc tgcgactaat gtcggcaaaa tcggcggttt
     2701 ggtcctgtcg gatcagcaga ttaaaggacg tgagccatca gcagcactcc ggacgcggcg
     2761 atggcggcgc caccgaggcg caagacctgc gccagctgca gccgcttggc agcgaggccg
     2821 atcgccaggc ccgttgtgat gagcgcgagc gtcgagctga tgaatccaat cgagtacgtc
     2881 agcagcgacg cgttcagcgg ccgctccgag acgtgcgcgt agccatgcag cattgcaaag
     2941 agcccgacca gcgccaccgg cgcgcccgcg ggcagccgcc cggcgcgggc gaccatcagg
     3001 ccgagcacca gcaccgacgc catgatgccc ggctcaacca gcggcatcgc caggccggtc
     3061 atcgcgagca ctcccccggc gatcatgaaa gcgacgaagg tcgcgggcat tgaccagatc
     3121 cagcgccggc ccatctgcgc gcccagcaac cccacggcga tcatcgccag caggtggtcg
     3181 atgcccgtga acgggtgcag cacgccgctg atccacggca ggtcggacga atgccccgga
     3241 tgagccgatg cgaccggcgc ggcgagcgcg aggacggcga aggtgctgac gatgctgagt
     3301 gcgttggtca acttggctga catgacttga tgctcctgca ttgggtcccg gacaaacagg
     3361 atagcacctt accgcgtacc cgtcaggctc gcacgatcaa ttttgaagga cgcgctatta
     3421 ggtactccgg tcgcacgccc tgagggagcc gagtcgtact cgttctggtt cggcccgagg
     3481 acagcccgaa cctcggcgtg tgaaatagtt ggcacctgag gagcggggat ggaaaaaccg
     3541 aacggggcga ggcg
//
LOCUS       DATIMH010000819         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4819974, whole genome shotgun sequence.
ACCESSION   DATIMH010000819 DATIMH010000000
VERSION     DATIMH010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4819974"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..470)
                     /locus_tag="VLI90_14185"
     CDS             complement(66..470)
                     /locus_tag="VLI90_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSV15404.1"
                     /translation="MLAVVTARSFSADAPEEWKAPPRAAKKQNPIPADEASNAAGKRI
                     YTKNCMACHGAGGRGDGPAAIACNPRPHDLSDPKIAGQTDGELFWKITTGKKPMPSYE
                     KQLTDDERWQAVNYLRVLTPRPANQPGEGANK"
     gene            complement(561..2000)
                     /locus_tag="VLI90_14190"
     CDS             complement(561..2000)
                     /locus_tag="VLI90_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HSV15405.1"
                     /translation="MTLQQAKIDTDAPAGFFGSRTGWRDLKQHILLEPVKGGSRWAAA
                     FGSLLLFVFILQVVTGVLLTMNYAPSTTTAWQSVRFIQDEVPHGRFIRAMHHWGSSAM
                     VILLLAHLLQVFIWGAYKRPRELTWMTGVLLLGATLGLAFTGYLLPWDEKAYWASKVG
                     LSIAGTVPLIGESMRQVLQGGSSMGNLTLTRFFALHGFILPGAIIALLSVHLYLFRLH
                     GVTTPWWENEADLLREQEPFWPGQVWKDAVVAFVFLFALAFWCRHFPAPLEDIANPAK
                     PYEARPEWYFMFLFQILKYFHGPYEVVGTFVLPAVFFGILFFWPLIDRTPQRDPRRRP
                     IAMGLLSLGTCGLIALTIFAVTTDTRMNKPAAAMTAEAQPAGPLQKLDAADLYAKNCL
                     VCHGVDGTGEQLRIALPTIPNLTDAAWQKTRTDADFATRIHDGKPPQMPAFADKLTPE
                     QIKALVVYDRAFASKPPPPSASQPLSNNP"
     gene            complement(2017..>2318)
                     /locus_tag="VLI90_14195"
     CDS             complement(2017..>2318)
                     /locus_tag="VLI90_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HSV15406.1"
                     /translation="ESRSVWVHRSDANSSDAAAVAVRSPICPHLGCPVMLTPDGTQFC
                     CPCHGGTFNASDGKRVAGPPPRDMDPMEFEIRDGHLWVRWQDFKIGVADRVAVTV"
BASE COUNT          415 a          730 c          767 g          406 t
ORIGIN      
        1 caacgcggtg gcggccgcta gcgcgaacgc gcctccgtac gcgcgcgcaa ggcgtttgcc
       61 ttggttcact tgttggctcc ttccccgggt tgattggcgg gacgcggcgt gagcacgcgc
      121 aggtagttca ccgcctgcca gcgctcgtcg tcggtgagtt gcttttcgta gctcggcatg
      181 ggcttcttgc cggtcgtgat cttccagaac agttccccgt cggtctgacc ggcgatcttc
      241 gggtcggaca aatcgtgcgg ccgcgggttg caggcgatcg ccgcgggacc gtcaccgcgg
      301 ccccccgcgc catggcaggc catgcagttc ttggtgtaga tccgtttccc ggccgcgttg
      361 gatgcttcgt cagcggggat tggattctgc ttcttcgccg cgcgcggcgg ggccttccat
      421 tcctcgggcg catcggcgga aaacgatcga gcggtgacga cggcaagcat ggccaccgtg
      481 gctatagcga tcgcaaacga ccggcggcgc gaacggcagg acgcgaacga ctcggactga
      541 tgcatgatga acctctcctt ctatggattg ttggataacg gctgcgatgc tgacggcggc
      601 ggtggcttcg atgcaaacgc gcggtcgtac accaccagcg ccttgatctg ctcgggcgtc
      661 agcttgtcgg caaatgccgg catctgcggg ggctttccat cgtggatgcg cgtggcgaag
      721 tcagcgtccg tgcgcgtctt ctgccacgcg gcgtcggtca ggttgggaat cgtcggtagc
      781 gcgatgcgca gctgctcgcc ggtgccgtcc acgccgtggc agaccaggca gttcttggcg
      841 tacagatcgg cggcatccag cttctgcagc gggccggccg gttgggcctc cgccgtcatg
      901 gcggcggcgg gtttgttcat gcgcgtgtcc gttgttactg cgaaaatcgt caatgcaatc
      961 aacccgcacg tgccgaggct caacaacccc atcgcgatcg gccggcggcg aggatcgcgc
     1021 tgtggtgttc gatcgatcaa tggccagaag aacaggatgc cgaagaacac cgccggcagc
     1081 acgaacgtgc cgaccacttc atacggcccg tggaaatact tcaggatctg aaacaggaac
     1141 atgaagtacc actcgggccg cgcttcgtat ggtttggctg gattggcgat gtcttccagc
     1201 ggcgcgggaa aatgccggca ccaaaacgcc agcgcgaaga ggaacacgaa cgcgacgacg
     1261 gcgtccttcc acacttgccc gggccagaac ggttcctgtt cgcgcagcag gtcggcttcg
     1321 ttttcccacc acggggtggt gacgccgtgc aggcggaaga ggtacaggtg aacgctgagc
     1381 agcgcgatga tggcgccggg caaaatgaac ccgtgcagcg cgaaaaagcg cgtgagcgtg
     1441 agattcccca tcgacgagcc gccctgaagc acctggcgca tcgattcacc gatcaacgga
     1501 acggtgccgg cgatgctcag gccgaccttg ctggcccaat aggctttctc gtcccaaggc
     1561 agcaggtatc cggtgaatgc caaaccaagc gtcgcgccca gcagcaatac gccggtcatc
     1621 cacgtcaact cgcgcgggcg cttgtacgcg ccccagatga agacctgcag caaatgggcc
     1681 agcagcagga tcaccatcgc actggaaccc cagtggtgca tcgcgcggat aaaccgcccg
     1741 tgcggcacct catcctggat aaaccgcacg ctctgccagg cggtggtggt ggacggcgca
     1801 tagttcatcg tcagcagcac accggtgacg acctgcagga tgaagacgaa cagcagcagg
     1861 ctgccgaacg ccgccgccca gcgcgaaccg cctttgaccg gctcgagcag gatgtgctgc
     1921 ttgaggtcgc gccacccggt gcgggatccg aagaaccccg cgggggcgtc ggtgtcgatc
     1981 ttggcttgct gtagcgtcat gtcgccctgc gccgcgttac acagttaccg ccacgcgatc
     2041 ggccacgccg atcttgaagt cctgccagcg aacccacagg tgtccgtcgc gaatttcaaa
     2101 ttccatcggg tccatgtcgc gcggcggggg accggcgact cgcttgccat cggacgcgtt
     2161 gaacgtcccg ccgtggcagg ggcagcagaa ctgcgtgccg tccggggtca acatgacggg
     2221 gcaacccagg tgcgggcaga tgggggagcg caccgcgacc gccgcggcgt cggagctgtt
     2281 ggcgtcagag cgatgcaccc agaccgatcg cgattcgc
//
LOCUS       DATIMH010000820         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3945195, whole genome shotgun sequence.
ACCESSION   DATIMH010000820 DATIMH010000000
VERSION     DATIMH010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3945195"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="VLI90_14200"
     CDS             <1..1501
                     /locus_tag="VLI90_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSV15407.1"
                     /translation="STSEPGVVDEYNTTFGIRTLRWDAARGFFLNGKPLKIKGTCNHQ
                     QHAGLGVALPDRMHEFRIAKLKELGSNAYRCSHYMVSPEVLDACDRHGLLVMAENRLA
                     GSSPEVLEQFSTMIVRDRNHPSIILWSIANEEHTIQWAIAGERIGRTMVELAHRLDPT
                     RAVTAAMHDRGLDEGFANIVDVHGWNYIAVGDIDAFHARRPEQPIVGSEEGSTVCTRG
                     VYADDKERGYVSAYDARKPKWGSNAERWWSYFAERPWIAGGFVWTGFDYYGEPIPYQW
                     PCTASHFGLMDLCGFPKDLYYYFKSWWSDEPTLHLMPHWNWPGREGEPIDVRCFSNCD
                     EVELLLNGRSIGRQAMPRNSHVRWDVPYEPGVLEARGFRAGQRRPMVTKRETTGAPAV
                     IRMTSDRPRISADGEDVAIITVWVADEAGRPVPTADDLLEFEITGEGRLIGVGNGDPS
                     SHESDKAPRRRLFNGLCQVLVQSTQKRGTIGVHARGQGLTDAALQVRSS"
     gene            complement(1643..2035)
                     /locus_tag="VLI90_14205"
     CDS             complement(1643..2035)
                     /locus_tag="VLI90_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15408.1"
                     /translation="METSHIPHRSQTPSSDPLTSGGGEPPGDRQVCLSETQQQALEWL
                     TNGGSVTEAAQFAGVARNTVSRWLHNDPDFRAVYDAWIEQTALVSTARMAAVQESAVD
                     VLIEAIRQRRDARVAMFVVKQTMDARKK"
     gene            complement(2373..3581)
                     /locus_tag="VLI90_14210"
     CDS             complement(2373..3581)
                     /locus_tag="VLI90_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HSV15409.1"
                     /translation="MSIAKPIEPPRPSAPPPAVSTALKHVALLVETSGSYGRGLLRGI
                     AKYNCAHGNWSTYFRPHGLADAPPKWLSQWRGDGLLVRIETKAVADLIARLKVPTVNL
                     RGTIPNLPFPYVSVDNAELAKLAFQHFLDRGLKHFALCNRPRGRNKAMDERGDRFERL
                     AKQAGYSCNIFIATSVDMDWEDEQEQMVKWIRALPKPVGIMAGNDERGLQVLDACRRC
                     GASVPHEVAVLGVDNDESLCDLAIPPMSSIDVNAEGIGYEAASLLDQMMNGKRVRSTS
                     IKIPPRGVVTRRSTDIIASEDEDVNRAVRYIREQACHGLRVVDVFNFLAMSRASLHDR
                     MKKITGLTVHQEIQRVRMARAKELLVMSPMTIKQIARESGFASVQYMTRVFRAATGET
                     PAQYRRQRAK"
BASE COUNT          677 a         1105 c         1247 g          688 t
ORIGIN      
        1 gagtacctcc gaacccggcg tggttgacga atacaacacc accttcggca tccgcacgct
       61 gcggtgggac gctgcgcgcg ggttcttcct caacggcaag ccgctgaaga tcaagggtac
      121 gtgcaatcat cagcagcacg cggggctggg ggtggcgctg ccggatcgga tgcacgagtt
      181 tcgcatcgcg aagttgaagg agctgggatc gaacgcgtat cgctgttcgc attacatggt
      241 gtcgccggag gtgctggatg cctgcgatcg gcacgggctg ctggtgatgg cggagaaccg
      301 gctggctggc agttcgccgg aggtgctcga gcaattctcg accatgatcg tgcgcgatcg
      361 caatcatccg agcatcatcc tctggtcgat cgccaacgag gagcacacga ttcaatgggc
      421 gatcgccggc gagcgcatcg ggcggacgat ggtggagctg gcgcatcggc tggatccgac
      481 acgcgcggtc acggcggcga tgcacgatcg cgggctggat gagggctttg ccaacatcgt
      541 cgatgttcac gggtggaact acattgcggt gggggacatc gatgcgtttc acgcgcgccg
      601 gccggagcag ccgatcgtcg gcagcgaaga aggcagcacc gtctgcacgc gcggcgttta
      661 cgccgacgac aaagagcgcg gctacgtcag cgcctacgat gcgcgcaagc ccaagtgggg
      721 atcgaacgcc gagcgatggt ggagctattt tgcggaacgt ccgtggatcg ccggggggtt
      781 tgtgtggacc gggttcgatt actacggcga gccgattccc taccagtggc cgtgcacggc
      841 gtcgcacttt gggttgatgg atttgtgcgg gtttccgaag gatttgtact actacttcaa
      901 gtcgtggtgg agcgacgagc cgacgctgca tctgatgccg cactggaact ggcccggccg
      961 cgagggcgag ccgatcgacg tgcgatgctt cagcaactgt gatgaggtgg aactgctgtt
     1021 gaacgggcga agcattggac ggcaggcgat gccgcggaat tcgcatgtgc gttgggacgt
     1081 gccgtacgag ccgggcgtgc tggaagcgcg cggctttcgc gcgggtcagc ggcgaccgat
     1141 ggtgacgaag cgcgaaacca ccggcgcgcc cgcggtgatt cggatgacga gcgatcggcc
     1201 gcgcatctcg gcggatggtg aagacgttgc cataattacc gtatgggtcg cggatgaagc
     1261 cggccggccc gtgccgacgg cggacgatct gctggaattt gaaatcaccg gcgaaggccg
     1321 gttgatcggc gtgggcaacg gtgatcccag cagtcacgag tcagacaagg cgccgcgtcg
     1381 ccggttgttc aacgggctgt gccaggtgtt ggtgcagagc acgcagaagc gcggcaccat
     1441 cggcgtgcac gcccgaggtc aagggctgac ggacgcggcg ctgcaggtgc gatcgtcgtg
     1501 aatttctcat gagcgcggcg acgtgccggc gcagttgaag ccgcttgcgg cttagcgttt
     1561 ttttaaatcc acaacagatg ctaagccgca agcggctgat acgacccgcg ccggggcacg
     1621 ccgcgctgcc agtgatgcgc gttcacttct tccgcgcatc catcgtctgc ttgaccacga
     1681 acatcgccac gcgcgcatcc cggcgttgcc ggatggcttc gatcagcaca tccacggcgg
     1741 actcctgcac ggcggccatg cgtgcggtgc tgaccagcgc ggtctgctcg atccacgcat
     1801 cgtaaacagc gcggaaatcc ggatcgttgt gcaaccatcg gctgacggtg ttgcgcgcca
     1861 cgccggcaaa ctgcgcggct tcggtcacgg acccgccatt ggtcaaccac tccagtgcct
     1921 gctgctgcgt ttcgctcaag caaacctgcc gatcacccgg cggctcgcca ccaccggagg
     1981 ttagagggtc ggaactgggc gtctgggaac gatgagggat gtgggaagtt tccatgcggt
     2041 gatcctaaca taatgcgaac aatcgatcaa tataattcat gtttatttga gaaataagca
     2101 acaaacggca aaatgttgca cgcagcgaaa agtgctacaa agtgcaacac ggtgcaacat
     2161 gcgctgacga agaatgagcg gtggaaggcg tattcagccg cttgcggcca agtggttttg
     2221 ccaggtgcaa aagacgctaa gccgcaagcg gccggagcgc gtccgcgccg ttccccctcg
     2281 cccacgagta catggacaag cgggccagac agacagtgat caacggtgca tttatcgagc
     2341 cagcgatcag cagtgcacgg cgtgcgcaat aatcatttcg cgcgctgccg ccgatactgc
     2401 gccggcgttt cgccggtcgc ggcgcggaag acgcgggtca tgtattgcac gctggcgaag
     2461 ccgctttcgc gggcgatctg cttgatggtc atcggcgaca tcaccagtag ttccttcgcc
     2521 cgcgccattc gcacgcgttg gatttcctgg tgcacggtca gaccggtgat ttttttcatc
     2581 cggtcgtgca atgacgcgcg cgacatggcc aggaagttga acacatccac gacgcgcagc
     2641 ccgtggcagg cctgctcgcg gatgtagcgg acggcgcggt tcacgtcttc gtcttcgctg
     2701 gcgatgatgt cggtcgatcg ccgcgtgacc acgccgcggg gtgggatttt gatcgacgtc
     2761 gagcgcaccc gcttgccgtt catcatctgg tccagcaggc tggcggcctc gtaaccgatg
     2821 ccctcggcgt tcacgtcgat gctggacatc ggcgggatcg ccaggtcgca aagcgattcg
     2881 tcgttatcca cgcccagcac ggcgacctcg tgcggcacgc tcgcgccgca gcgccggcag
     2941 gcgtccagca cctgcagccc gcgctcgtcg ttgcccgcca tgatgcccac gggcttgggc
     3001 agcgcgcgga tccacttcac catctgctcc tgctcgtctt cccaatccat atcgaccgag
     3061 gtggcgatga agatgttgca ggaatagccg gcttgcttcg ccaggcgctc gaagcggtcg
     3121 ccgcgttcgt ccatcgcctt gttccgcccg cgcgggcgat tgcacagcgc gaagtgcttg
     3181 agcccgcgat cgaggaaatg ctggaacgcg agcttggcca gctcggcgtt gtctacgctg
     3241 acatatggga acggcaagtt cgggatcgtc ccgcgcaggt taacggtcgg cactttcaat
     3301 cgcgcgatga gatccgccac cgccttggtt tcgatgcgaa cgagcagccc gtcgccgcgc
     3361 cattggctga gccacttcgg cggcgcatcg gccagcccgt gcgggcggaa gtaggtggac
     3421 cagttgccgt gggcgcagtt gtacttcgcg atcccgcgca gcagcccccg gccgtacgag
     3481 ccggaggttt ccaccagcag cgcgacgtgc ttcaaagcgg tagaaaccgc cggcggcggg
     3541 gcggacggac gaggcggttc gatgggtttg gctatggaca tggatgtggt ctgatgtaga
     3601 cggtgcgagc gctgtggtcc cctcgcccgg tactccggga gagggttagg gtgagggtga
     3661 tttcaattat caccggcaat cgacgctcga atcaccctca ccccagccct ctcccgg
//
LOCUS       DATIMH010000821         3766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2793880, whole genome shotgun sequence.
ACCESSION   DATIMH010000821 DATIMH010000000
VERSION     DATIMH010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793880"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1179
                     /locus_tag="VLI90_14215"
     CDS             157..1179
                     /locus_tag="VLI90_14215"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV15410.1"
                     /translation="MKKELGIGILLVVLVIVLRLLNPRFLSPINVQNLSNLVGIFGIF
                     SIGLGVIIITGGIDLSVGSVFALQGVLLSMMLVQGHWPWPVAALSAIAIVVLLGVFHG
                     FIITVVKLQPFIVTLCGLLFYRGLARFIANDETKGFGSDSFGLLRPMATKVLYIGDWG
                     IGMPFVLLMIIAVIMWVVLHRSVYGRYLYAVGRNEEAARYSGINTKLVIGGAYALSML
                     LASITGILLAFYTNSISPNDHGNSYELYGIAAAVLGGCSLRGGEGSIIGIILGTILLQ
                     VLRNLVTLLHIPSSLDLAVMGAVIFIGVTIDQLLGTRKKRTKTPALVQQTDAIPAKPA
                     VGGPTP"
     gene            1237..2385
                     /locus_tag="VLI90_14220"
     CDS             1237..2385
                     /locus_tag="VLI90_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15411.1"
                     /translation="MKSIDGQPSWRIASRDVEAYVTVNGGHLAPVTFDRRGMKIRPYS
                     IAPWGRDRSAPRSLPMIIRALRGDFFCMPFGGNEQPFGKERFPVHGETANAKWKLQSQ
                     SCLGGVSDLRLTLRTKIRTGQVQKLIRLIDGHNAVYCRHKITEASGPMNFGHHAMLKF
                     PDEAGSGVVSTSPFVFGQVYLGAFEKPEEGGYSALKRAAEFTSLEAVPGLDGSAVDVT
                     RYPARRGFEELLMVINDPKVPLAWTAATFPKERYAWFALKDPAVLRGTILWVSNGGRH
                     YAPWSGRHINVMGLEDVTGHFHDGIAASAAPDNSLRRRGYPTSVQLSPDFATVVNYVM
                     AIAKIPVGFDRVERIEPSDDGKSVTLISRTGKRATAPINVDFVRNADH"
     gene            2707..3663
                     /locus_tag="VLI90_14225"
     CDS             2707..3663
                     /locus_tag="VLI90_14225"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSV15412.1"
                     /translation="MRVFVTGAAGFIGSRLSKALIARGDEVIGFDNLNDYYPLVHKER
                     HLADLLPSKRKFAFIKGDLRDAGQLRELFQQHKPDAVAHIAAMAAVRYSVQFPLIYGD
                     VNVQGSVNLLDAARQIGHPTCVLASTGSVYGADTPVPFVETAAAVHPLAPYPASKRAM
                     ELFAHTYAHLWKLPTTVLRFFNVYGPHGRPDMMPWQWSQQILAGETLTLYNSGKLKRD
                     WTYIDDIVAGFISALDKRLANEIINLGCGNPVDNLQIVSILENLLGKKAKTVDTPTPP
                     SEPLITYANVGKARELLGYQPKVSVDEGLKRFIEWMRGEKLI"
BASE COUNT          713 a         1179 c         1179 g          695 t
ORIGIN      
        1 agaaaaaacg ccgcgaggcc gcggcggcta aacgacagac ggctactaga cagacggcta
       61 aaccacagac ggctacacaa tagacggtta acgactaatc agctaaaccc ccgcgccgcg
      121 tccactatcg cccgcgggag aggaagcgct tcttccatga aaaaagaact gggcatcggc
      181 atcctcctgg tcgtgctggt catcgtgctg cggctgctga acccgcgctt cctcagcccg
      241 atcaacgtgc agaacctgtc gaacctcgtc ggcatcttcg gcatcttcag catcgggttg
      301 ggcgtcatca tcatcaccgg cgggatcgac ctgtccgtcg ggtcggtctt cgcgctgcaa
      361 ggcgtgctgc tgtcgatgat gctcgtccaa ggccactggc cctggcccgt cgcggcgctg
      421 tcggccatcg ccatcgtcgt gctgctcggc gtgttccacg gtttcatcat caccgtcgtg
      481 aagctgcagc cgttcatcgt gacgctgtgc ggcctgctct tctaccgcgg gctggcgcgc
      541 ttcatcgcga acgatgaaac caagggattc ggcagcgatt catttggtct gctgcgcccg
      601 atggctacca aagtgctgta catcggcgac tggggcatcg gcatgccgtt cgtgctgctg
      661 atgatcatcg cggtcattat gtgggtcgtg ctgcaccggt ccgtgtacgg tcgctatctg
      721 tacgccgtgg gccgcaacga agaagcggcg cgatattcgg gcatcaacac gaagctcgtg
      781 attggcgggg cgtacgcgct gtcgatgctg ctggcgtcga tcaccggcat cttgctcgcg
      841 ttctacacca actcgatctc cccgaacgat cacggcaact cgtacgagct gtacggcatc
      901 gccgcggcgg tgcttggcgg gtgcagcctt cgcggcggtg agggctcgat catcggcatc
      961 atcctgggca ccatcctcct gcaggtgctg cgcaacctgg tgacgctgct gcacattccc
     1021 agttcgctgg acctggcggt catgggcgcg gtgatcttca ttggcgtgac gatcgaccag
     1081 ctgctgggca cgcgcaagaa acggacgaag accccggcgc tcgtccagca gaccgacgcg
     1141 attcccgcca agcccgccgt gggtggaccg acgccgtaat tgtgcccgcc caccgtcgcc
     1201 ttgcggcgac gcgcgctgtt gcggcaaaat gcgcgcatga aatcgatcga cggccaaccc
     1261 tcctggcgca tcgcctcgcg cgacgttgaa gcgtacgtca ctgtcaatgg cggacatctg
     1321 gcgccggtga cgttcgaccg ccgggggatg aagatccggc cgtattcgat tgcgccatgg
     1381 ggcagggatc gatcggcccc gcggtcattg ccgatgatca tccgcgcgct gcgcggcgac
     1441 ttcttctgca tgcccttcgg cgggaacgag cagccgttcg gcaaagagcg ctttcccgtc
     1501 cacggtgaaa ccgccaacgc gaagtggaag ctgcaatcgc aaagctgcct tggcggggtc
     1561 agcgatcttc ggctgaccct ccgcacgaag attcggacgg ggcaggtgca gaaactgatc
     1621 cgcctgatcg atgggcacaa cgcggtgtat tgccggcaca agatcaccga agcgagcggg
     1681 ccaatgaatt ttggccacca cgcgatgctg aagttcccgg acgaagccgg ctcgggcgtc
     1741 gtctccacca gcccgttcgt cttcggccag gtctatctcg gcgcattcga gaagccggaa
     1801 gaggggggat actcagcgct gaagcgcgca gcggaattca catcgctcga agctgtcccg
     1861 ggtttggacg gcagcgcggt cgatgtcacg cgctatcccg cgcggcgtgg gtttgaagag
     1921 ctgctcatgg tgatcaacga cccgaaggtg ccgctcgcat ggacggcggc gacgttcccg
     1981 aaggagcgct acgcgtggtt cgcgctcaaa gaccccgcgg tgctgcgcgg cacgatcctc
     2041 tgggtcagca acggcggccg gcactatgcc ccatggagcg gccggcacat taacgtgatg
     2101 gggctggaag acgtcaccgg ccatttccac gacggcatcg ccgcgtcggc ggcgccggac
     2161 aattcgctgc gccggcgggg gtatccgacg agcgtgcaat tatcgccgga cttcgcgacg
     2221 gtcgtgaatt acgtgatggc catcgcgaaa atccccgtcg ggtttgatcg cgtcgagcgg
     2281 atcgaaccga gcgacgatgg aaagtcggtg acgctgattt cgcgcaccgg caagcgcgcg
     2341 acggcgccga tcaacgtcga tttcgtgcgc aacgccgatc attgatttcc attgcgtaac
     2401 ggcaccttgc gtagcggtag ccccgaaggg gcacgtgttt cgatgtacga gcgggtcgaa
     2461 aacagattga accacgaagc tcacgaagag cacgaagaag aaaaaaggca aatgttttcc
     2521 tctgcttcgt gctcttcgtg agcttcgtgg ttcaacttcc ttattccgaa aacacgtgcc
     2581 cctgcggcgc taccgctaca caacagatcg cgaacgcgat tgcgaagtac ccgcttgcga
     2641 gcgcggcgcg ccgccctcag aatacattgc aactacaaca gtacgctttc tctctggagc
     2701 aatttgatgc gcgtgttcgt tacaggagcg gcggggttca tcgggtcgcg gctcagcaaa
     2761 gcgctgatcg cccgcgggga cgaagtcatc ggcttcgaca atctcaacga ctattatccg
     2821 ctcgtacaca aggagcgcca cctggcggac ctgctgccga gcaagcggaa gttcgcgttc
     2881 atcaagggcg acctccgcga cgcggggcag ctgcgcgagt tgttccagca gcataaaccg
     2941 gatgccgtcg cccacatcgc cgccatggcg gcggtgcgat acagcgtgca gttcccgctg
     3001 atctacggcg acgtgaacgt acagggctcc gtgaatctcc tggacgcggc gcgacagatc
     3061 ggccatccca cctgcgtgct cgcctcgacc ggcagcgtgt acggcgccga cacgcccgtg
     3121 ccgttcgtcg aaaccgccgc cgcggtgcat ccgctggcgc cgtatccggc gagcaagcgc
     3181 gcgatggaac tgttcgcgca cacctatgcc catctgtgga agctgccgac gaccgtgctg
     3241 cgtttcttca acgtctacgg cccgcacggc aggccggaca tgatgccctg gcaatggtcg
     3301 cagcagatcc tcgccggcga aacgctcacg ctctacaaca gcggaaagct gaagcgcgac
     3361 tggacgtaca tcgacgatat cgtcgccggc tttatctcgg cgctggacaa gcgcctcgcg
     3421 aacgagatca tcaacctcgg ctgcggcaac ccggtcgata acctgcagat cgtcagcatc
     3481 ctcgagaacc tgctcggcaa gaaggcgaag acggtggaca cacccacgcc gccgtcggag
     3541 ccgctgatca cgtacgcgaa tgtcggcaaa gcgcgcgagt tgctgggtta tcagccgaag
     3601 gtgtcggtgg atgaagggct gaagcgcttc atcgaatgga tgcgcggtga gaagctgatc
     3661 tgattcgcga cttgctcaat ccgtctgtag ctgcgacgcc tgttagctag atggcgcggc
     3721 ggtcgcttgc ggtttcgcag acggacaagc cgccgtcact ggtctg
//
LOCUS       DATIMH010000822         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2215728, whole genome shotgun sequence.
ACCESSION   DATIMH010000822 DATIMH010000000
VERSION     DATIMH010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2215728"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VLI90_14230"
     CDS             complement(<1..300)
                     /locus_tag="VLI90_14230"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSV15413.1"
                     /translation="MQALPESMVGLRSAYENQQKQFSRAFAVLRDAIQQQAFPGAALA
                     VTHRGQLVASEGFGNFTYDESAPVVTRDTVFDLASLTKVVATTSMAMLLFERGVLG"
     gene            456..>2126
                     /locus_tag="VLI90_14235"
     CDS             456..>2126
                     /locus_tag="VLI90_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSV15414.1"
                     /translation="MVLLGAALPCVAQRTAPPPSAPDRFVPLDLILQNAVANKLTPGV
                     VCIVGHNGVVIYRKAYGFRSINPILEPMTVDTVFDMASLTKVMATTGSIMRMVQLGQI
                     KLNDPVAKYIPDFAQNGKEDVTVRQLLTHYSGLRADLELRPPWSGMDEGYRRANAEKL
                     INPPGSTFLYSDINFIVLGELVQKMSGVTLNQYAETFIFGPLGMNTTRFLPPMSLLGR
                     TAPTQINEKTGEVLRAVVHDPTARAMGGVAGHAGLFSTADDTAKFAQAMLNGGAPVWS
                     REIVEKMTTPQQPPNLTVVRGLGWDIDSPFSSNRGDLLPVGSYGHTGFTGTSLWIDPT
                     TKSYIILLTNAVQLKEGSVIALRTEVATAVASVLNLSLPQEQKYRLSRITGYNEAAAA
                     SRRIVARNGTVLLGIDQLESSDFEPLRQFGTNKPRLALLTSQTALDTLGHRTIDVLAH
                     GSGLHLDAIFTTDAPAPDSPAGKDGPSGVPLYNLAGETEASRRPSPEVLRNVDGVVVD
                     LPDTGARISHLVSTLGYLLEAAAKAGKPVVVLDRPNPLNGAYVQGAISD"
BASE COUNT          423 a          644 c          650 g          409 t
ORIGIN      
        1 accgagtacg ccgcgctcaa acagaagcat cgccatgctg gtagtcgcca cgaccttggt
       61 cagggaagcc aggtcgaaca cggtgtcacg cgtgaccact ggcgcggact cgtcgtaggt
      121 aaaattgccg aacccttcgg aggcgaccag ttgtccccgg tgggttacgg caagcgcggc
      181 tccgggaaaa gcttgctgct ggatggcatc gcgcaggacg gcaaacgcgc gcgagaattg
      241 cttctgctgg ttctcgtacg cggagcgaag gcccaccatc gactcgggta aggcttgcat
      301 agcactctcg gcggcaggac agccgcgccg aatcttataa tacgaatctt gatgataagt
      361 cggtatttac tcgtaggtca gcatatctcg ccggtatcag acggaccatg cccattttga
      421 gactgcgggt ctggcctttc cattgtgtgg catggatggt gctgcttggc gccgcgctcc
      481 cttgcgtcgc tcagcgtaca gcgccgccgc caagcgctcc cgatcgcttt gtaccgctgg
      541 acctgatcct gcaaaacgcg gttgccaaca agttgacgcc gggtgtggta tgcatcgtcg
      601 gccacaacgg ggtggtgatt taccgcaagg cgtacggctt tcgttccatc aatcccatcc
      661 tggagccgat gacagtcgac accgtgttcg acatggcgtc gctcaccaag gtgatggcga
      721 ccaccgggtc gatcatgcgc atggtgcaat tggggcagat caagctgaac gatccggttg
      781 ccaagtacat acccgacttc gcacagaacg gcaaagaaga cgtcaccgtc cgtcagttgc
      841 tgacgcacta ttccgggctg cgcgccgatc tggaattgcg tccgccctgg agcggcatgg
      901 acgaaggtta tcgccgcgct aatgccgaaa agctgatcaa tccgccgggc tcgacctttc
      961 tgtacagcga catcaacttc atcgtgcttg gcgaactggt gcagaagatg agtggcgtga
     1021 cgctgaacca gtatgccgaa actttcatct tcggcccgtt ggggatgaat acgacgcgct
     1081 tccttccgcc tatgtccctg ctgggtcgaa ccgctcccac gcagatcaat gaaaagactg
     1141 gcgaggtctt gcgcgccgta gtacacgacc ccacggcgcg cgcgatgggc ggagttgcgg
     1201 gacatgcggg actgttctct accgccgacg atacggcgaa gttcgcacag gcgatgctca
     1261 acggcggcgc gcccgtctgg tcgcgcgaga tcgtggagaa gatgaccacc ccgcaacagc
     1321 ctccgaacct cacggtagtg cggggcctcg gttgggacat cgacagcccg ttttcctcta
     1381 atcgcggcga cctgttaccg gtgggctcgt acggccacac cggcttcacc ggaacgtcgc
     1441 tgtggatcga tcccacgacg aaaagctaca tcatcctgct gaccaacgcg gtgcaactga
     1501 aagagggaag cgtcatcgcg ttgcgcaccg aagtggcaac ggcggtggca tcggtcttga
     1561 atttgagtct gccgcaagag cagaaatacc ggctgtcgcg tattacgggc tacaacgaag
     1621 ccgcagctgc gagccggcgc attgtcgccc gcaacggcac cgtgctgctc gggatcgatc
     1681 aactcgagtc cagcgacttc gagccgttgc gtcagttcgg aaccaacaag ccgcgcctcg
     1741 ccctgctgac cagtcaaacg gcgcttgaca ctctcggaca tcgcaccatc gacgttctcg
     1801 cgcatggaag tgggctgcat ctggatgcga ttttcactac ggacgcgcca gcccccgata
     1861 gtccagccgg caaagatggg cccagcggcg tccccctcta caatctcgcg ggggaaacgg
     1921 aggcgagccg gcggccgtct ccggaggtgt tgcgcaacgt cgacggggtg gtcgttgatt
     1981 tgccggacac cggtgctcgc atttcacatc tggtctcgac cctggggtat ctgctggaag
     2041 ccgcagccaa ggcgggcaaa cctgttgttg tgctggaccg cccgaatcca ctgaacggag
     2101 cgtatgtgca gggggcgatc tccgat
//
LOCUS       DATIMH010000823         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1064030, whole genome shotgun sequence.
ACCESSION   DATIMH010000823 DATIMH010000000
VERSION     DATIMH010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064030"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VLI90_14240"
     CDS             complement(<1..471)
                     /locus_tag="VLI90_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15415.1"
                     /translation="MAQQEMSFAQALTEAKQIIVQQSNRIKTDLEKIRSLQETVASQT
                     SALAASERTINQQATALAEHDEQIRSLTARLAEATQAREQAETIIDRQGQRLTQSQTA
                     IQTLEKHVADQTQRMNELMQEIEATRQQLPTQEDEQALAALSTLLSTKKISVPAA"
     gene            699..1082
                     /locus_tag="VLI90_14245"
     CDS             699..1082
                     /locus_tag="VLI90_14245"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HSV15416.1"
                     /translation="MPIRTRRWDDPREEGDGQRILITRYRPRGLPKADETWDVWMPDL
                     GPSKGLHAAVYGKVGTGIAWATYRARYLAEMSAQREKIAELAQRVATGETITLLCSSQ
                     CDREARCHRSILKELIEAAAAPQQS"
     gene            complement(1136..1378)
                     /locus_tag="VLI90_14250"
     CDS             complement(1136..1378)
                     /locus_tag="VLI90_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15417.1"
                     /translation="MAEKSEFDRGGDGMTRGGLDRYLGPPAQDYSHLPPSMADDKVDP
                     DDAGREIDCDGTDAKEAVCEIAKLEEEVRERGASAT"
     gene            1459..2253
                     /locus_tag="VLI90_14255"
     CDS             1459..2253
                     /locus_tag="VLI90_14255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HSV15418.1"
                     /translation="MSLPVLKGAAAVGRDDLLRFFHKIQLEYRRHLGEETQLDCGTAI
                     VNAELKSVSDANGVMDAALPPDTAAAAALDSVTAHFAQSSSLCQRWVLNPSAPLEQTQ
                     PLVEELTRRGHARQTMDILHLPRLPQSPVREVGGLRIVPARASYRHVRELAEEAGQSA
                     DAALLHLDDPRFEAMLALKEGQAVAIAGVLGSGENAGVQQLFVSERFRRQGIGRTMMS
                     RVLEICVRSLYRHILLSVEPQNTAAQGLFRGLGFEKVGEWVEFVSS"
     gene            complement(2485..>3025)
                     /locus_tag="VLI90_14260"
     CDS             complement(2485..>3025)
                     /locus_tag="VLI90_14260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV15419.1"
                     /translation="ILNVLDERYKLLIWGRGDRAANAARLGVRLHQPDMVKLAEPLLG
                     RAIEFEQLLPATDACIVTARAPVATLPIAICMAAGLPIVSTVTYTAAELLEDRHTALM
                     VPKPSPQLLAQRVLDLRGDSALQWSIADMARTEAYEFFSLTRMMNQYRAVYHQMAADE
                     AVEVPEAAAGAGLRFHGRG"
BASE COUNT          533 a          958 c          968 g          566 t
ORIGIN      
        1 ggcggcgggg acgctgattt tcttcgttga cagcagcgtg gacaacgccg ccagcgcctg
       61 ttcgtcttcc tgcgtcggca gctgttgacg ggtcgcctcg atctcctgca tcagctcatt
      121 catgcgctgc gtttgatcgg caacatgctt ctccagcgtc tggattgccg tctgcgactg
      181 cgtgagccgc tggccttggc gatcgatgat cgtctccgcc tgctcgcggg cctgggtcgc
      241 ttcggccaac cgcgcggtga gcgagcggat ctgctcatcg tgctcggcca gcgcggtcgc
      301 ctgctggttg atcgtgcgtt cgctggcggc cagcgcgctg gtctgcgacg cgaccgtctc
      361 ctgcagcgat cgaatctttt ccagatccgt tttgatgcga ttggattgtt gaacgatgat
      421 ttgcttggct tcggtcagtg cttgggcaaa ggacatttcc tgctgtgcca tggcaagcct
      481 ccggtatagg ggttcgcgcg actcggtttg tggaaaacaa cgctaccgac gagccgccgc
      541 gcgccactcg ttcactcgaa tgggagcggg acaaccccgt ctggacataa gaagtgtcgg
      601 ctatgccggc tgcggggatt agaaaagtgt cataaccatg tgaaatgtct tccgcacgtc
      661 cgataaccgc gcctggatgc taagcgtata atgtgatcat gccgattcgt acccggcgct
      721 gggatgaccc cagggaagaa ggcgacggcc agcgcatcct gatcacgcgc tatcgcccgc
      781 gaggtttgcc gaaggcggac gagacgtggg acgtgtggat gccggacctt ggcccgagca
      841 aggggttgca cgccgcggtt tacggcaaag tcggcaccgg catcgcgtgg gcgacgtatc
      901 gcgcgcgata cctggcggag atgagcgcgc agcgcgagaa aatcgccgaa ctcgcacagc
      961 gcgtcgccac cggcgagacg atcacgctgc tgtgctcctc acaatgcgac cgcgaagcgc
     1021 gatgtcaccg ctcgatcctg aaggagttga ttgaagcagc agcagcaccg cagcaatcgt
     1081 agcagccgcc aaaaaaagcc cacggctctg ccgtgggatc ttactcaata aaccgtcaag
     1141 tcgcgctcgc cccgcgctcc cgcacctcct cctcgagttt cgcaatctca caaaccgcct
     1201 ccttcgcatc cgtcccgtcg cagtcgatct cccgccccgc gtcgtccggg tccaccttgt
     1261 catccgccat cgacggcggc agatgcgaat aatcctgcgc cggcggaccc agatatcgat
     1321 ccagcccacc gcgcgtcatc ccgtcaccgc cgcggtcgaa ttcgctcttt tcagccatgg
     1381 ctttcctttc acaaaaccga tggactaatc gctgcaatga ccgtgccgtc gaacagaagc
     1441 ggacgctaca atgtggcgat gtcgttgccg gtgctcaaag gcgcagccgc ggtgggtcgg
     1501 gatgatctgc tgcggttctt ccacaagatt cagctggagt atcgccggca cctgggcgaa
     1561 gaaacgcagc tggactgcgg caccgcaatc gttaacgccg agttgaaaag cgtaagtgat
     1621 gccaatggag tgatggacgc ggcgttgccg cccgacactg ccgctgccgc cgcgctcgat
     1681 tcagtgacgg ctcacttcgc gcaatcatct tccctctgcc agcgctgggt tttgaatccg
     1741 tccgcgccac tggaacaaac gcagccgctc gtagaggaat taacgcggcg cggacatgcg
     1801 cgccagacga tggacatcct gcatctgccg cggctgccgc agtcgccggt gcgcgaggtg
     1861 gggggtttga ggatcgtgcc ggcgcgggcg agctatcgcc acgtgcgcga gctggcggaa
     1921 gaggcgggcc aatccgccga cgcggcgctg ctgcatctcg acgacccgcg cttcgaggcg
     1981 atgctggcgc tgaaggaggg ccaagccgtc gcgatcgccg gcgtgctcgg ttccggcgaa
     2041 aacgccggtg tgcagcagct gttcgtcagc gagcgtttcc ggcgacaagg catcggccgc
     2101 acgatgatga gccgcgtgct cgagatctgc gtgcgctcgc tgtatcggca catcctgctg
     2161 agcgtcgaac cgcagaacac cgcggcacag ggcctctttc gcggactggg attcgagaag
     2221 gttggggagt gggtggagtt cgtgtcatcc tgaacgaagc acgtactcgt gcgcagtcga
     2281 aggatctagc ccagaagtga gtgaaccata gcttgcgcga tcggcgcggc agtcgcttgc
     2341 ggtttcgcag accgagcggc gtgatctgcc aagccgcgag cgactgccgc gccgatggcg
     2401 tgagcaatat ttcatagact tccggcaaga tccttcgcct ccggcgagta cgcctgcgct
     2461 cagaatgaca cgcacgcgtg cgcctcaccc gcggccgtgg aaacgcagtc cggcgccggc
     2521 ggcggcttcg gggacttcca ccgcttcatc ggccgccatc tggtgataga ccgcgcgata
     2581 ttggttcatc atccgcgtca gcgaaaagaa ctcgtatgct tcggtccggg ccatgtcggc
     2641 gatcgaccac tgcagcgcgc tgtcgccgcg caggtcgagc acgcgctgcg ccagcaattg
     2701 cggtgacggc ttgggcacca tcagcgcggt atgccggtct tccaacaact ccgcggcggt
     2761 gtacgtgacg gtcgagacga tcggcaaccc cgcggccatg cagatggcga tcggcagcgt
     2821 cgcgaccggt gcgcgggccg tgacgatgca cgcgtcggtg gcgggcagga gttgctcgaa
     2881 ttcgatcgcg cggcccagca gcggctcggc gagctttacc atgtccggtt gatgcaaccg
     2941 cacgcccagc cgtgccgcgt ttgccgcgcg atccccgcgg ccccagatca gcagcttgta
     3001 tcgctcgtcc agcacgttca ggatg
//
LOCUS       DATIMH010000824         6570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2215833, whole genome shotgun sequence.
ACCESSION   DATIMH010000824 DATIMH010000000
VERSION     DATIMH010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2215833"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            389..3043
                     /locus_tag="VLI90_14265"
     CDS             389..3043
                     /locus_tag="VLI90_14265"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HSV15420.1"
                     /translation="MLAPVAGVCAGAAPASTQSDTNRVLRTFDFEERQLGNDEDLPMH
                     WDKVDGPDLPHYVTGRLSTDRHRSGQYSFRMDLNGGSCVYRYDPGQIRVQRNAHYRVE
                     GYCQTTALPNARARLTAYFTDLDHHPIASTVRHSELYAFHTSDTPGATDDEADWHPLH
                     VELASDDPNAAYLVVEMSLLQPNMFSNSSLGQRSLFVQDIHGTAWFDDITISQVPQIT
                     ISTDRPGNIFRRGEPLKLSVLVNDRFTDDLAAQLIVRDALGKSIFQRTGALDMSAAET
                     LGPGQKRMSLLLPDVPPGWYQASLVMSSHGVFVGEQALNLVLLADTAPPIMPDPRFGV
                     IATDLPFEGWSELPQILPFLSAGRVKLAVWSGAGDVQQMDSAAFDHLLESLQELGITP
                     TACLISPPPKLVERLNGKTWLQLLKAPPEEWETQLAYLVSRHATHLDRWQLGADGSDE
                     FVTNRDMRRVYSLVYQQFARLVQKPDLAMPWPAWYDLDGELPATVALSVPPSVLPAQL
                     PLYMQDIKNHDGHNLSLTLQLLDRAQYGREMQIRDLAERVVYALAADAKRIDLPLPFT
                     VRQQNEALVKDPQELLIIMRTLITTLGGSTFRGKVPIADNIEAFLFDKNGQGILVLWD
                     KGNDAGDRQLALNLGDRPLRMDLWGNVTPLLHNGQGDSMRVKMNLGPMPIILIDIDGQ
                     IAQLRASVAFDQPLIESSFEPHLRHVRFNNPYHQAIGGTFKLRAPAGWTLNPPTFSFT
                     LNPGETFNREVTIEFPYNSYAGAKTINAEFNVQAERNSTFTVPLTLTLGLSDVGMQSL
                     ALRDGKDVVVQQMITNYGEKPINYTAFAVYPGQARQERLVTNLGPGRTTMKKYRLTNV
                     TIAKGAKVRAGVKELDGTRILNEEIEIQ"
     gene            3165..3578
                     /locus_tag="VLI90_14270"
     CDS             3165..3578
                     /locus_tag="VLI90_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV15421.1"
                     /translation="MPYVLVVDDHADTRESLCAFLRKIGHDVGVAGNGDEALTSVLER
                     LPDLIVLDLYMPQMDGASLLDVLRSYTRLQSVPVVVWTAYPDSPMVARARKNGVTEVL
                     SKGTTSFEDILSAINRLLPPPPQNGRSGSAPGLWH"
     gene            3703..5166
                     /gene="xseA"
                     /locus_tag="VLI90_14275"
     CDS             3703..5166
                     /gene="xseA"
                     /locus_tag="VLI90_14275"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HSV15422.1"
                     /translation="MTGAGGFFDMREKLIAPRRARAAAGDGASPSSATTGKPLSVTQV
                     TKLIERAITAGVPEALSVQGEVSNFNLNRASGHAYFTMKDASACIDCVMFRSEFARLK
                     FKPGDGMEMIATGNVRVYPQRGRYQLYVNQLHPLGQGALELAFQQLRSKLEAEGLFNA
                     DRKRPVPPYPRRIVLVTSRQGAALQDMLKVLRRFPWIELMIYHVPVQGDGCGQQIAAA
                     IAHLNRQVNHVGGADLILLGRGGGSLEDLWGFNEEGLARAIVASRLPIITGIGHEVDV
                     SIADLVADHWAHTPTEAAQVATAYWRAAPNLLVERTVRLRRQMATLVQSARQRLTAIA
                     RHEVFRRPTDRIDDLRMTLDDRQRALMLAMNHRLHACHRELREKTERLERQGPAVQVT
                     RVRARVDEIAGRMSRALNTSIQRRADRVNASAALLDAVSPHRVLARGYSLTRVKKTGA
                     IVRKAEQVKPGDRLVTRFQDGEVESIADDAKQPRLFE"
     gene            complement(5180..>6570)
                     /locus_tag="VLI90_14280"
     CDS             complement(5180..>6570)
                     /locus_tag="VLI90_14280"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HSV15423.1"
                     /translation="MGIAHYIATEGKDRRLYDAARRLADCWCDHIGPAPKQAWFDGHQ
                     EIEQALVRLGRFVNDTDGAGKGQKYIDLAKFLLDCRGGGSDYDQSQYPVTQQYQAVGH
                     AVRASYTYSAMADVSMETRDPAYYSAIFSIWDNLVNRKLYITGGIGSGDTSEGFGADY
                     SLPTASAYCESCSNCGLLFMNYKLNLATRDAKYADVYETAIYNAILGDVDLPAKNFTY
                     TNSLDSSAARYGWHNCPCCVGNIPRTLLMLPTWMYAKSDDAVYVNLFGGSTVNVGKLA
                     GTEVEMVQATDYPWNGKVAITVNPKAEAAFEVCVRSPRRDVSALYTAEPAGDGITSLI
                     VNGEKMNAVADADGYVHIKRSWKPGDKIELDLPMPVQQIKASDKVASTRGRMALRRGP
                     LIYNVESVDQNVELLVDRNATMDSQWSPELLDGVITLHGRYTDGTPLIAVPNYARLNR
                     GGRSLVWMKGQE"
BASE COUNT         1214 a         2161 c         2020 g         1175 t
ORIGIN      
        1 actgtgacac acaacaaccc gcgccaccgc cgacttctct tcccaggttt tcgcatcgag
       61 tcgcccacgt cttagatagg agctgctgcc accgagccgc ttttaagtga gaccgcaggg
      121 tggcagtgca actctgcgac agcttctttt tagtgggaac cggcccgcgc gcgttgtgag
      181 atgcgcattg attccccagc caagccctcc gcgcgtaagc aaacttctaa caagaccggg
      241 gttggggatg caacgaataa atgaatcgtg cgacgattca agccattcca gcagggcaag
      301 tccccaagtg ctcaactcat tgaggccgtt caaccgatag tgacattatc ggaccatgcg
      361 cctcgccacg ctcatcacta tcgctgcctt gcttgcaccc gtcgccggtg tctgcgcggg
      421 cgcggctccg gcaagcaccc agagcgacac caatcgcgtt ctgcgcacgt tcgacttcga
      481 agaacgccag ctcggcaacg atgaagactt gccgatgcac tgggacaagg tggacgggcc
      541 ggacctgccg cactatgtga ccgggcggtt gtcgaccgac cggcatcgca gcgggcagta
      601 cagctttcgc atggacctca acggcggcag ctgcgtttat cgttacgacc ccggccagat
      661 ccgcgtgcag cgcaacgccc actaccgcgt ggaaggttac tgccaaacca ccgcgttgcc
      721 caacgcgcgg gcgcggctca ccgcgtactt caccgatctc gatcatcacc ccatcgcctc
      781 gaccgttcga cattccgaac tctacgcctt ccacaccagc gatacgcccg gcgcgacaga
      841 tgatgaagcc gactggcatc cgcttcacgt cgagctggcc agcgacgatc cgaacgccgc
      901 gtacctcgtt gtcgaaatga gcctgcttca gcccaacatg ttttccaaca gctcgctcgg
      961 ccagcggtcg ctgttcgtgc aggacatcca cggaaccgcg tggttcgatg acatcacgat
     1021 ctcgcaggtg ccgcagatca cgatctcgac cgatcgcccg ggcaacatct ttcgccgcgg
     1081 tgagccgctc aagctttcgg tgctggtgaa cgaccgtttc accgacgacc tggcggccca
     1141 gctgatcgtc cgcgatgcgc tgggcaagtc gatcttccag cgcaccggcg cgctggatat
     1201 gtcggcggcc gagacgctgg ggccggggca gaagcgcatg tcgctgctgc tgcccgatgt
     1261 cccgccaggg tggtatcagg cgtcgctggt gatgagcagc catggcgtgt tcgtgggcga
     1321 gcaggcgctg aacctggtgc tgctggcgga caccgcgccg ccgatcatgc ccgatccgcg
     1381 ctttggtgtg atcgcgacgg atctgccgtt tgaaggctgg agcgaattgc cgcagatcct
     1441 gccgttcctg tcggccggtc gcgtgaagct ggcggtgtgg agcggcgccg gcgacgtgca
     1501 gcagatggac tcggccgcct tcgatcacct gctggaaagc ctgcaggaac ttggaatcac
     1561 gcccaccgcg tgtcttatca gcccaccacc aaaattggtg gaacgattga acggcaagac
     1621 ctggctgcaa ttgctcaaag ctccgccgga agaatgggag acgcagctgg cgtatctggt
     1681 gtcgcggcac gcgacgcatc tggatcgctg gcaacttggc gccgacggca gcgatgaatt
     1741 cgtcaccaac cgcgacatgc gccgcgtgta cagcctggtc taccagcagt tcgcccggct
     1801 ggtgcagaag ccggatctcg cgatgccctg gcccgcgtgg tacgacctcg acggcgagct
     1861 gccggcgacg gtcgcgctgt ccgtcccgcc atcggtgctg ccggcgcagt tgccgctgta
     1921 catgcaggac atcaagaacc acgatgggca caacctgtcg ctgacgctgc agttgctcga
     1981 tcgcgcgcag tacggccggg agatgcagat tcgcgatctc gccgagcgcg tcgtttacgc
     2041 gctcgccgcc gacgccaagc gcatcgacct gccgctgccg ttcacggtgc ggcagcaaaa
     2101 cgaagcgctg gtgaaggacc cgcaggaact gctcatcatc atgcgcacgc tgatcaccac
     2161 gctgggcggc agcacgttcc gcggcaaggt cccaatcgcc gacaacatcg aagcgttcct
     2221 gttcgacaag aacggccagg gaattttggt cctgtgggac aagggcaacg acgcgggcga
     2281 ccgccagctg gcgctgaacc tgggcgatcg cccgctgcgc atggacctgt ggggcaacgt
     2341 tacgccgttg ctgcacaacg ggcagggcga ttcgatgcgg gtgaagatga acctcggccc
     2401 gatgccgatc atcctgatag acatcgacgg gcagatcgcc cagctgcgcg ccagcgtcgc
     2461 gttcgatcag ccgctgatcg aatccagctt cgagccgcac ctgcggcacg tgcggttcaa
     2521 caacccttat caccaggcga tcggcggcac gttcaagctt cgcgccccgg ccggttggac
     2581 attgaatcca cccacgttca gcttcacgct gaatcccggt gagacgttca accgcgaggt
     2641 gacgatcgaa tttccataca acagttacgc cggggcgaag acgatcaacg ccgagttcaa
     2701 cgtgcaggcg gaacgcaaca gcacgttcac cgtaccgctc acgctgacgc tggggctgag
     2761 cgatgttggc atgcagtcgc tggccctgcg cgatggcaaa gacgttgtcg tccagcagat
     2821 gatcaccaac tatggcgaga agccgatcaa ctacaccgcc tttgccgttt atccgggcca
     2881 agcacggcag gaacggctcg tcaccaacct cggccccggt cgcacgacga tgaagaagta
     2941 tcgattgacc aacgtcacta tcgccaaagg cgcgaaagtt cgcgcgggcg tgaaagagct
     3001 ggacggcacg cgcatcctga acgaggaaat cgaaatccag tagaacgggg cacttgtgcg
     3061 gtagcggctg cttcgtagcc ccttcgtatc gcgcgctcgc ttttctctcc tgcagatttt
     3121 cacccgttcc tcattcgatc tgccggcact aaaatgcggc tgttgtgcca tacgtacttg
     3181 ttgtagatga ccatgccgac acgcgcgaaa gtttgtgcgc gttcctgcgc aaaattggcc
     3241 atgacgtggg cgtcgccggc aacggcgatg aggcgctgac gtcggtgctg gagcggctgc
     3301 cggacctgat cgtcctggac ctgtacatgc cgcagatgga cggcgcgagc ctgctcgatg
     3361 tgctgcgctc gtacacgcgg ctacagtccg tgccggtcgt cgtctggacg gcatatcccg
     3421 acagcccgat ggtcgcccgc gcgcgcaaga acggcgtcac cgaggtcctc tccaaaggca
     3481 cgacgtcgtt cgaagacatc ctcagcgcca tcaaccgcct gctcccgcca ccgccgcaga
     3541 acggacggtc cgggtcagcg cccggcctgt ggcattaaca gccgcttgcg gcttagcatc
     3601 ttttccccgc ttcgcgaaca catgctaagg ccgcgagcgg ctgtggagcc cacagcatcc
     3661 cactcctcga agcgcacacg ttccatcggt atacttcacc ccatgactgg tgccggagga
     3721 ttcttcgaca tgcgcgaaaa gctaatcgcc ccacggcggg cgcgcgccgc ggctggcgat
     3781 ggcgcgtccc catcctccgc caccaccggc aagcccctct ccgtcacgca ggtgacgaag
     3841 ctgatcgaac gcgccatcac cgccggcgtg cccgaagcgc tctcggttca gggagaagtc
     3901 agcaatttca atctcaatcg cgcatcgggc cacgcgtact tcacgatgaa ggacgccagc
     3961 gcctgcatcg actgcgtgat gttccgcagc gaatttgccc ggttgaagtt caagcccggc
     4021 gacggcatgg agatgatcgc gaccggcaac gtgcgcgtgt atccgcagcg cgggcgatac
     4081 caactctacg tcaaccagct gcacccgctg ggacagggcg cgctggagct ggcgttccaa
     4141 cagttgcgca gcaagcttga agccgagggc ctattcaacg ccgaccgcaa gcgcccggtc
     4201 ccgccgtacc cgcggcggat cgtgctcgtc accagccggc agggcgcggc ccttcaagac
     4261 atgctcaaag tcttgcgccg ctttccgtgg atcgagctga tgatctacca cgtcccggtg
     4321 cagggcgacg gctgcggcca acagatcgcc gcggcaatcg cgcatctgaa ccggcaggtc
     4381 aatcacgtcg gcggcgcgga cctgatcctg ctcggccgcg ggggcggctc gctggaagac
     4441 ctgtggggat tcaacgaaga aggcctcgcc cgcgcgatcg tcgcatcgcg gttgcccatt
     4501 atcacgggca tcgggcacga agtggacgtg tcgatcgccg acctcgtcgc cgatcattgg
     4561 gcgcacacac cgacggaagc cgcgcaggtg gccaccgcgt attggcgggc ggccccgaac
     4621 ctgctcgtcg agcgcaccgt gcggttgcgc cggcagatgg cgacgctggt ccaaagcgcc
     4681 cggcagcgcc tgacggcaat cgcccgccat gaagtcttcc gccggccgac ggatcgcatt
     4741 gacgatctgc gcatgacgct ggatgaccgc cagcgtgcgc tgatgctggc gatgaaccac
     4801 cgcctgcacg cctgtcaccg cgaattgcgc gaaaagacgg agcgcctgga acgccaaggc
     4861 ccggctgttc aagtcactcg tgtgcgcgca agggtagatg aaatcgccgg acgaatgtcg
     4921 cgcgcgctga acacgtcgat ccaacgccgc gccgaccgcg tcaacgcctc agcggcgttg
     4981 ctcgacgcgg tcagcccgca tcgcgtgctt gcccgcgggt attcgctgac gcgtgtgaag
     5041 aagactggcg cgatcgtccg caaagccgag caggtgaagc ccggcgaccg cctcgttacg
     5101 cgcttccagg acggcgaagt cgagtcgatc gccgacgacg caaagcagcc gcgcttgttc
     5161 gagtgatgcg tggcgaaacc tactcctgtc ccttcatcca aaccagcgaa cgtccgccgc
     5221 gattcagtct cgcgtaattc ggcaccgcga tcagcggagt gccgtcggtg taccggccgt
     5281 gcagcgttat cacgccgtcg agtagctcag gtgaccactg cgaatccatc gtcgcgttgc
     5341 ggtccaccag cagctcgacg ttctggtcca ccgactcgac gttgtaaatc agcggccccc
     5401 ggcggagagc cattcgcccg cgcgtgctgg cgactttgtc gctggccttg atctgctgta
     5461 ccggcatcgg caggtccagt tcgatcttgt cgcccggctt ccaggaacgc ttgatgtgaa
     5521 cgtaaccatc ggcgtccgcc accgcgttca ttttttcgcc gttcacgatc agtgaagtga
     5581 tgccgtcgcc ggccggctcg gcggtgtaga gggcgctcac atcccgccgc ggggaacgca
     5641 cgcagacttc aaacgcggct tcggctttcg gattgaccgt gatcgccacc ttgccgttcc
     5701 acggataatc cgtcgcctgc accatctcga cctcggtgcc tgccagcttg ccgacgttca
     5761 ccgtgctgcc gccgaacagg ttcacgtaca ccgcgtcgtc ggacttggcg tacatccacg
     5821 tcggcagcat cagcagcgtg cgcgggatgt tgcccacgca gcacggacag ttgtgccagc
     5881 cgtatcgggc ggccgacgag tcgagcgaat tcgtgtacgt gaaattcttc gccggcagat
     5941 cgacgtcgcc gaggatcgcg ttgtagatgg ccgtctcgta aacgtcggcg tacttcgcgt
     6001 cgcgggtcgc gaggttcagc ttgtagttca tgaacagcag gccgcagttc gagcacgatt
     6061 cgcaatacgc gctggccgtg ggcagcgaat agtcggcgcc gaaaccctcg gatgtgtcgc
     6121 cggaaccgat gccgccggtg atgtacagct tgcggttgac caggttgtcc cagatggaaa
     6181 agatcgcgct gtagtacgcg gggtcgcgcg tttccatcga gacgtcggcc atcgccgagt
     6241 acgtgtacga cgcccgcacc gcgtgaccga cggcctggta ctgctgcgtc accgggtact
     6301 gggactgatc gtaatcgctg ccgccgccgc ggcagtcgag caggaatttc gccaggtcga
     6361 tgtacttctg gcccttgccg gcgccgtcgg tgtcgttgac gaatcggccg aggcgaacca
     6421 gcgcttgttc gatttcctgg tgcccgtcga accacgcctg cttcggcgcg ggaccgatgt
     6481 gatcgcacca gcaatccgcc agccggcggg ccgcatcgta caaccggcgg tccttgcctt
     6541 cggtggcgat gtaatgggcg atgcccatct
//
LOCUS       DATIMH010000825         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1933715, whole genome shotgun sequence.
ACCESSION   DATIMH010000825 DATIMH010000000
VERSION     DATIMH010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933715"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VLI90_14285"
     CDS             complement(<1..1027)
                     /locus_tag="VLI90_14285"
                     /inference="COORDINATES: protein motif:HMM:NF027404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15424.1"
                     /translation="MAYQELRGNSYLFGANAPFIEALYERYLEDPAAVEPRWRTYFDE
                     LQKLDDGARDVAHAPVQEHFAQLARSPRAAAVANTGESSQLSEKQYGVLQMIGAYRFQ
                     GARIADVDPLHRQDPPAIAELDPAFYGLSEADMETVFHTGSLVAPRDMKLKDILQLLR
                     DTYCRTLGTEYMYIADIPQKRWIQERLEPSRSTPAYDGAYKKHILERLTAAETLERYL
                     HTRYVGQTRFSLEGGDSLIPMLDNLLQRAGGAGVQELVIGMAHRGRLNVLVNTLGKMP
                     KDLFAEFEGKHDDLKLAGDVKYHDGFSSNVMTPGGPLHLSLAFNPSHLEIVDPVVEGS
                     VRARQHRR"
     gene            complement(1059..>1843)
                     /gene="gltA"
                     /locus_tag="VLI90_14290"
     CDS             complement(1059..>1843)
                     /gene="gltA"
                     /locus_tag="VLI90_14290"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HSV15425.1"
                     /translation="TLVAMAYKYSVGQPFVYPQNDLSYSANFMRMMFSTPCSPYKVND
                     VLVRALDRILILHADHEQNASTSTVRLSGSSGANPFACIAAGIACLWGPAHGGANEAA
                     LNMLKEIGSVEKIGEFVKQVKDKNSGVKLMGFGHRVYKNYDPRAKLMRETCHEVLAEL
                     GLENDPLFKLAMALEKVALEDEYFVSRKLYPNVDFYSGIVQSALGIPVSLFTGIFAMA
                     RTVGWIAQWNEMIADPDQKIARPRQLYTGYGKRDFVPLTKRK"
BASE COUNT          339 a          575 c          571 g          358 t
ORIGIN      
        1 cgcgccggtg ctggcgtgcc cgcaccgaac cttcgacgac gggatcgacg atctcgaggt
       61 gcgacggatt gaaggccagc gataaatgca gcgggccgcc gggcgtcatg acgttcgagg
      121 aaaacccgtc gtggtacttg acgtcgcccg cgagttttag atcatcgtgc ttgccttcga
      181 attcggcaaa caggtccttc ggcatcttgc cgagcgtatt gacgagaacg ttcagccgtc
      241 cgcgatgcgc cataccgatc acgagctcct gcaccccggc gccgcccgcg cgctgcagca
      301 aattgtcgag catcggaatc aggctgtcgc cgccttcaag cgaaaaccgc gtctgcccga
      361 catagcgcgt atgcaggtac cgctcaagcg tctcggcggc ggtcaaccgc tcgaggatgt
      421 gctttttgta ggcaccatca taggccggcg tcgaccgcga cggctcgagc cgctcctgga
      481 tccagcgctt ttgcggaatg tcggcgatgt acatgtactc ggtgccgagg gtgcggcagt
      541 acgtgtcccg caacagttgc aggatgtctt tcaacttcat gtcgcgcgga gccactagcg
      601 acccggtgtg aaacaccgtt tccatgtcgg cttcgctcaa gccataaaac gcgggatcca
      661 gttcagcaat ggcaggcggg tcctggcgat gcagcggatc gacgtcggca atgcgcgcgc
      721 cctggaaccg gtaggcgcct atcatctgca gaacgccgta ttgcttttca ctgagctgcg
      781 aactctcgcc cgtattggcc accgccgccg cccgcgggct gcgcgccaac tgcgcgaagt
      841 gctcctgcac cggcgcatgc gcgacatcgc gcgcgccatc gtccagtttt tgcagctcgt
      901 cgaaataggt gcgccagcgc ggctctacgg ctgccgggtc ttcgagataa cgctcgtaca
      961 gcgcttcgat gaaaggcgcg ttcgcgccga acaggtacga attgccgcgc agttcctggt
     1021 aagccatgac gcttgaagcc ggcgttcgga cgctgcgctt acttgcgctt ggtcaggggc
     1081 acgaagtcgc gcttgccgta accggtgtag agctggcgcg gacgcgcaat cttctggtcc
     1141 ggatcggcga tcatctcatt ccactgcgca atccagccca cggtgcgggc catggcgaag
     1201 ataccggtga acagcgaaac cgggatgccc agcgcagact gcacgatgcc ggagtagaag
     1261 tcgacgttcg ggtacagctt gcgcgacacg aagtattcgt cttccagagc gaccttttcc
     1321 agcgccatcg ccagcttgaa cagcgggtcg ttttccaggc ccagttcggc cagcacttcg
     1381 tggcaggttt cgcgcatcag cttggcgcgc ggatcgtagt tcttgtacac gcggtgaccg
     1441 aagcccatca gcttcacgcc cgagttcttg tccttcacct gcttgacgaa ttcgccgatc
     1501 ttctcgaccg agccgatttc cttgagcatg ttgagcgctg cctcgttggc gccgccgtgc
     1561 gccgggcccc acaggcaggc gatgccggct gcgatacagg cgaacgggtt ggcgcccgac
     1621 gaaccggaca ggcgcacggt cgaggtcgat gcgttctgct cgtggtccgc atgcaggatc
     1681 aggatgcgat ccagcgcgcg caccagcaca tcgttcacct tgtagggcga gcacggcgtg
     1741 gagaacatca tgcgcatgaa attcgcgctg tacgacagat cgttttgcgg atagacgaaa
     1801 ggctggccca ccgaatactt gtaagccatg gcgaccaggg tcg
//
LOCUS       DATIMH010000826         6601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2506020, whole genome shotgun sequence.
ACCESSION   DATIMH010000826 DATIMH010000000
VERSION     DATIMH010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506020"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VLI90_14295"
     CDS             complement(<1..366)
                     /locus_tag="VLI90_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15426.1"
                     /translation="MTGHNTGVAGKRGNSIRGNGQKARRSALSLCAAAATVSALGIFA
                     PKARAANVTWLGNNSGSWSDSGNWTTLPASGDSLIFGVAGPNGSSLSDDLITPGTFSD
                     LTFTSAAPGYSIFPSSGNGF"
     gene            complement(780..2024)
                     /locus_tag="VLI90_14300"
     CDS             complement(780..2024)
                     /locus_tag="VLI90_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSV15427.1"
                     /translation="MENRRPATTRKNHTSGKPSQPPALGTPAAAADRQLVALRVPAWS
                     DWDRQVIRGVQRFAHGRPRWRLYVESGAASALRIATADLRLDGLITGALRDPAPVWQR
                     LLKRMRGRMVAITSAVPLRFASIARVQVDDDKIAATIGRHLISSGFRNLAYYGSRRPG
                     LEDLRLNAITTFARSRGYPCHLLPAQAGQGAVTMPQRLRWIRQLPKPIGIVTWNMSEA
                     RDLLAALDRAGVAVPEDVAVVSWDDDPMLAETLEPTISAAVLPAERLGFEAAQLLDRL
                     MNGEAAPAAPTRIEPTGVMQVRQSSDVSTLADRDAHLAMQYIREHGTEPIKVSHVAAA
                     LRLSRRSLEIAFKNVAGKTLHEAITEVRMERARQLLAETDWPLSRVAEQSGVGTEETL
                     RRLFLATEQLTPGAYRSRFSGV"
     gene            2212..3279
                     /locus_tag="VLI90_14305"
     CDS             2212..3279
                     /locus_tag="VLI90_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HSV15428.1"
                     /translation="MRRLNGLYILDTDALDQIYGAAERAAIERHVDLVALPQTRHSIA
                     ENPALLRNVDVIFSGWGGPQLSEALLDAAPKLKAFFYGAGTVGHLITEAVWERGIQVT
                     TANAANAVPVAEYTVAMITLALKQVWRFQRATRDARTFMRNDSDIAGAYGSSVGLISL
                     GTCGRAVLSRLRGFDVNVVAYDPFVNRAEAEKVLRIGLVALPELFRRCDVVSLHSPNL
                     EETRGMITGELLMSMKPGATFINTARGDIVREDEMLAVAARRPDLQFIVDVTEPEPPP
                     ADSPFYTLPNVLLTPHVAGSIGPECRRMGRYMVEELERFVKQQPLKWLVTQESCRHST
                     HRPVVVTTYITPKPRKPAPAV"
     gene            3395..4906
                     /locus_tag="VLI90_14310"
     CDS             3395..4906
                     /locus_tag="VLI90_14310"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSV15429.1"
                     /translation="MPLRRLLGICLIALAMSRAIAGAATEPTGNAVPPEPLPRVPQTQ
                     MGIAQLPNEKLQWLLDDKFGMFIHWGLYSGIGRGEWVMEHEAIAPEAYRKYAYPESGD
                     AHFDAADYHPQAWAQLAKDAGMRWMCLTARHHEGFCLFDSPHPNAFTSMQTLHRDLLA
                     EYVTACRAAGLKVGIYYSPLSWRYPGYYDVTGTHCAPNKFGYTTDPAHAENARLMKEE
                     NYVNVKKLLTGYGPIDHIYWDGGWLGQQGSDADGAFFHEPGIYLDPSNRWPIPVQYQD
                     IEQSTGKALGIMGMVRKYQPNAITNLRYGWMGDILEEEGSRETTGPIRSSAVYDKNLT
                     MQNGGWGYNAKSVARGDVMSRDQIIRYLASCVVRDMTLLVNVAPDRHGVIPEIEQTRL
                     REVGQWLAKTGDAIYRTRGGPWQPVDHQYGYCYKDRTVFVHLLKDYAGDKFTLPPLGT
                     LKVVRVYDVFSGAPLQFDAGATVTIRRIDRSESAADSVIAVVYDADVRSVWAK"
     gene            complement(5123..6022)
                     /locus_tag="VLI90_14315"
     CDS             complement(5123..6022)
                     /locus_tag="VLI90_14315"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV15430.1"
                     /translation="MTATLATSTPTAVQSRRTARLNLPALRALFTFTLRQHRHGKRWM
                     VMAALMLLPAGLAVLVRCTAHGVPSVGLEFIFAFMFIPQALLPLAALIYASGIIQDEQ
                     EDQTITYLLIRPLPKWAIYIVKLLATLTVTVALAILFTALTYVAIYAGANADGINDVT
                     TRCLKACALHSLAIVAYGCLFGLLSILTQRTLIVGIIYIAVVEGLFANLAFGIRLITV
                     IYYARLLAYRTLTFIIPTPTGTQDFAADAWQLDIANDPTLQEHPQFRTCLLVLLGASI
                     SFAVLAAVLCAHREFHVKTPEKR"
     gene            complement(6200..>6601)
                     /locus_tag="VLI90_14320"
     CDS             complement(6200..>6601)
                     /locus_tag="VLI90_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15431.1"
                     /translation="RLGAAGKSVVVSSHVLHEVQSLTQNIILLNHGRLLAEGQVRQIR
                     DLIDKHPHHIVLISDGARKLAARLVSWPDVEGVRVLPDDRGLMVETRVPDAFYSRLPE
                     LSLEDGTAIREVYSDDDNLEAVFKYLVNKSA"
BASE COUNT         1234 a         2170 c         2074 g         1123 t
ORIGIN      
        1 gaagccgttt ccactcgatg gaaaaattga atagccaggc gcggcggacg tgaacgtcag
       61 atcagagaac gtccccggcg ttatcaggtc gtcgctgagg ctcgatccgt tgggaccggc
      121 gacgccgaag atcagcgaat caccgctggc gggcagggtc gtccagttgc cggagtcgga
      181 ccagctgccg ctgttgttcc cgagccaggt gacattggcc gcacgggcct tgggggcgaa
      241 gatgcccagc gcgctcaccg tcgccgcagc cgcacacagg ctgagcgccg accgacgggc
      301 cttttgacca ttgccacgaa tgctgttgcc ccgcttgccc gccacgcccg tattgtgacc
      361 cgtcattgat ctctcctcgc tcaagttgaa tcgcgcgtgc gtcgtcgtag gacgcccgca
      421 gatgcgccgt gctcgccgca gatatgaatc cgttactgga ttgcccgccg cttaagaccg
      481 tcgggtgtgc gacctccgca ttgcctcacc accgccgccg ccgatcgcat atttcacgca
      541 ccgccgccct gcgcgcgcac ggattgaaaa gaaatgccgt gtgacctgcg cgagtgagcg
      601 ggcggaaatc tgccgcagct tgattccgat acactacaat gcagcggccg gcggcgttat
      661 ccacacttcg cgcaaaagga acgaaaaaca acctcagttt gcgtcgagcg cttcaacgtg
      721 gcatgagcgt atcgcccgtg cgggatgcag cggatttcaa aaaagattgc ggtcccgctt
      781 taaaccccgc tgaagcggga tcggtaggcg ccaggtgtca gctgttcggt cgccaggaac
      841 aagcggcgca gcgtctcctc cgtccccacg ccggattgct ccgccacgcg cgacagcggc
      901 caatcggtct cggccaacag ctggcgggca cgctccatgc gcacctcggt gatcgcttcg
      961 tgcagcgtct tgcccgccac attcttgaac gcgatctcca gactccggcg cgacagccgc
     1021 aacgctgcgg caacgtggct gaccttgatc ggctccgtgc cgtgctcgcg gatgtattgc
     1081 atcgccagat gcgcatcgcg atcggccagc gtcgaaacat ccgatgactg ccgcacctgc
     1141 atcacgccgg tcggctcgat ccgcgtgggc gccgccgggg cggcctcgcc attcatcagg
     1201 cggtcgagca actgcgcggc ttcgaagccc aatcgctccg ccggcaacac cgcggcggag
     1261 atcgtcggtt ccagcgtctc ggccagcatc gggtcgtcgt cccacgagac caccgccacg
     1321 tcttccggca ccgcgacgcc cgcgcgatcc agcgccgcta acagatcgcg cgcttcgctc
     1381 atgttccagg tgacgatgcc gatcggcttg ggcagctggc ggatccagcg caggcgctgc
     1441 ggcatcgtaa ccgcgccttg ccccgcctgg gccggtagta gatggcacgg gtatccccgc
     1501 gaccgcgcaa atgtagtaat tgcattcagc cgcaggtcct ccagccctgg ccggcgcgag
     1561 ccgtagtacg ccaggttgcg aaaaccgctg gaaatcagat gccgcccaat cgtggcggcg
     1621 atcttgtcgt catccacctg cacgcgcgcg atcgacgcaa accgcagcgg caccgccgag
     1681 gtgatcgcga ccatccgccc gcgcatccgc ttgagcagcc gctgccacac cggcgcggga
     1741 tcgcgcagcg cgccggtgat caacccgtcc aagcgcaggt ccgccgtcgc gatccgcagc
     1801 gcgctggcgg cgccgctttc gacgtacaac cgccagcgcg gccggccgtg cgcgaagcgc
     1861 tgcacgccgc gaatcacctg gcggtcccag tccgaccacg cggggacgcg cagcgcgacc
     1921 aattgccgat ccgccgccgc ggcgggcgtg cccagtgcgg ggggttgcga cggtttgcct
     1981 gaggtatggt tttttcgggt cgttgccggt cgtcggtttt ccatatgagc cctgtcgaag
     2041 gattgtggcg atcaaatatc gcgaccaatg gtcacggctt taccggtcgc cgcaaagtgt
     2101 gggcacattt tatccatttc gcgcaaactg tgtgtgcggt tcatcacgcc aatcccgtac
     2161 cttcgatggc gcccggcgag gacggctttt gcgcgggcgg acgaacgtta catgcgtaga
     2221 ctcaacgggc tatacatcct ggacaccgat gcgctggatc agatttacgg ggccgccgag
     2281 cgcgccgcga tcgagcggca cgtcgatctg gtcgcgctgc cgcagacgcg gcactcgatc
     2341 gccgaaaacc ccgcgctgct gcgcaacgtg gacgtgatct tcagcggctg gggtggaccg
     2401 cagctgagcg aggcgctgct cgacgccgcc ccgaagctca aggcgttctt ctacggcgcc
     2461 ggcaccgtcg ggcatctgat caccgaagcc gtctgggagc gcggcatcca ggtcaccacc
     2521 gccaacgccg ccaacgccgt gcccgtcgcc gagtacaccg tcgcgatgat cacgctggcg
     2581 ctgaagcagg tctggcggtt tcagcgcgcc acgcgcgacg cccgcacatt tatgcgcaac
     2641 gatagcgaca tcgctggcgc ctatggcagc agcgtcggcc tgatctcgct gggcacctgc
     2701 ggtcgggccg tgctctctcg cctgcgcggg ttcgacgtga acgtggtggc gtacgacccg
     2761 ttcgtcaatc gcgccgaagc ggaaaaagtc ctgcgcatcg ggctcgtcgc gctgccggaa
     2821 ctattccggc gctgcgacgt cgtctcgctg cattcaccga acctggaaga aacccgcggc
     2881 atgatcacgg gcgaactgct gatgtcgatg aagccgggtg cgacgttcat caacaccgcc
     2941 cgcggggaca tcgtgcgcga ggacgagatg ctcgccgtcg ccgcgcgccg accggatctg
     3001 cagttcatcg tggacgtgac cgagcccgag cccccgccgg cggattcgcc gttctacacg
     3061 ctgccgaatg tgctgctgac gccgcacgtc gccggttcga tcgggcccga gtgccgccgg
     3121 atggggcgtt acatggtcga ggaactcgag cgcttcgtga agcagcagcc gctgaagtgg
     3181 ctggtgacgc aagaatcctg ccggcacagc acccatcggc cggtcgttgt gacgacatac
     3241 atcacaccca aacctcgcaa gccggcgcca gcggtttaat atctcggcgc gcgccgccac
     3301 gccgggtcgg tcttaccccc tctcccggag taccgggcga ggggaccgga acgaaaccga
     3361 ctggtggcgt gaagctcgat caatggagga ttcgatgccc cttcgtcgcc tgctcggaat
     3421 ttgcctcatt gccttggcga tgtctcgcgc cattgcaggc gcggcgactg agccaacggg
     3481 aaatgccgtt ccacccgagc cattgccgcg cgtgccgcag acgcagatgg gcatcgccca
     3541 actgcccaac gaaaaactcc aatggctgct cgatgacaag ttcggcatgt tcatccactg
     3601 gggcctgtac tccggcatcg gccgcggcga atgggtgatg gagcacgagg cgatcgcgcc
     3661 cgaggcatac cgcaaatacg cttaccccga gagcggcgac gcgcacttcg acgcggccga
     3721 ttatcacccg caggcttggg cgcaattggc gaaggacgcg ggcatgcgct ggatgtgtct
     3781 caccgcccgc caccatgagg gattctgcct gttcgacagc ccgcatccca acgcgttcac
     3841 cagcatgcag acgctgcatc gcgatctgct ggccgagtac gtgacggcgt gtcgagccgc
     3901 gggtctgaag gtcggcattt actattcgcc gctcagctgg cgataccccg gttactacga
     3961 cgtgactggc acgcactgcg cgccgaacaa gttcggctac acgaccgatc cggctcatgc
     4021 cgagaacgcc cggctgatga aggaggagaa ctacgtcaac gtcaaaaaac tcctcaccgg
     4081 ttacggcccg atcgatcaca tctactggga cggcggctgg cttggccagc aaggctccga
     4141 cgcggacggc gccttctttc acgagcccgg catatacctg gacccgagca accgctggcc
     4201 gatcccggtt caatatcagg acatcgaaca atcaaccggc aaggcgctgg gcatcatggg
     4261 catggtgcgc aagtatcagc cgaacgcgat caccaacctc cgctacggct ggatgggcga
     4321 catcctggaa gaggaaggct cgcgcgaaac gaccggcccg attcgcagca gcgccgtcta
     4381 cgataaaaac ctgacgatgc agaacggcgg atggggatac aacgccaagt ccgtcgcccg
     4441 cggcgacgtg atgagccggg atcagatcat ccgctacctc gccagctgcg tcgtgcgcga
     4501 catgacgctg ctggtgaacg tcgcccccga tcgccacggc gtcatccccg aaatcgaaca
     4561 gacgcgcctg cgcgaagtcg gccagtggct cgcgaaaacc ggcgacgcca tctacagaac
     4621 ccgcggcggg ccgtggcagc cggtcgatca ccaatacggc tactgctaca aggaccggac
     4681 cgtcttcgtg catctgctga aggactacgc gggggataag ttcaccttgc ccccacttgg
     4741 cacgctgaag gtcgtgcgcg tttacgatgt cttctccggc gcaccgctac agttcgacgc
     4801 cggcgccacc gtcacgattc gccggatcga ccgcagcgag tctgccgctg attccgtgat
     4861 cgcggtggtt tacgacgcgg atgtgcgaag cgtttgggcg aaatagtcgc gtaccagtta
     4921 tcctgagcgc caatcagttt atcctgagcg gagcacgtac tcgtgcgcag tcgaaggacc
     4981 tggccgagaa gccagtgaac gactaatcgg aacacgccgc cgccgccgct tgcggcttcg
     5041 caaacgcaca agctgcggtc attggtctgc gaaaccgcaa gcgactaccg cgccgatcgc
     5101 accagccacc gttcacagac gcctaccgct tctccggcgt cttcacatga aactcgcggt
     5161 gggcgcacag caccgccgcc agcaccgcga acgagatgct ggcccccagc agcacgagca
     5221 aacaagtacg gaactgcggg tgttcctgca gcgtggggtc gttggcgatg tccagctgcc
     5281 acgcgtcggc ggcgaagtct tgcgtgccgg ttggggtggg gatgatgaac gtgagcgtgc
     5341 gataggccag caggcgcgcg taatagatga cggtgatgag ccgaatgccg aaggcgaggt
     5401 tggcgaagag gccctcgacg acggcgatgt agatgatgcc gacgatcagc gtgcgctgcg
     5461 tgagaatgct caacaggccg aaaaggcagc cataggcgac aatcgcaagc gagtgcaggg
     5521 cgcaggcctt gaggcagcgc gtcgtcacgt cattgatccc atcggcgttc gcgccagcgt
     5581 agatcgcgac ataggtcagc gcggtgaaga ggatcgccag cgcgacggtg acggtcaatg
     5641 tcgccaatag cttcacaata tagattgccc acttcggcag cgggcggatg agcaggtagg
     5701 tgatcgtctg gtcttcctgt tcgtcctgga tgatgccgga ggcgtagatg agcgctgcca
     5761 gcggcagcag cgcctgcggg ataaacatga aggcgaagat gaattcgagc cccacgctgg
     5821 gcacgccatg ggcggtgcag cggacgagaa ccgccagccc cgcggggagc agcatcaacg
     5881 cggccatgac catccagcgc ttgccgtgac gatgctggcg cagcgtgaaa gtgaaaagcg
     5941 cccggagcgc ggggaggttg agacgtgccg tccggcgcga ttggactgcc gttggtgtgg
     6001 atgtcgcgag cgttgcagtc atttagaagt ccatccgccg cttgcggctt agcatctgtt
     6061 ccttcgcgcg gcggaaaaac atgctaagcc gcaagcggcg gtgacgcctt ttcctaagat
     6121 tgaattcacc gacttctcgg caagatcctt cgactcccgc gagcacgcct gcgctggagg
     6181 atgatccgcg ctcgcacgtt cacgcgctct tgttcaccag atatttgaat accgcttcga
     6241 gattatcatc atctgaatac acctcccgaa tcgccgtccc gtcttccagc gacaactccg
     6301 gcagccggct gtaaaacgca tccggcacgc gcgtctccac catcagcccg cgatcgtcgg
     6361 gcaacacgcg aacgccttcg acgtccggcc aactcacgag gcgggccgcc agctttcgtg
     6421 cgccgtccga gatcagcacg atgtgatgcg gatgcttgtc gatcaggtcg cggatctgcc
     6481 gcacctgccc ctcggccagc agccggccgt ggttcagcag gatgatgttc tgcgtgagcg
     6541 actgcacctc gtgcagcacg tgactggaga cgacgacgct tttccccgcg gcgccgagcc
     6601 g
//
LOCUS       DATIMH010000827         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3663761, whole genome shotgun sequence.
ACCESSION   DATIMH010000827 DATIMH010000000
VERSION     DATIMH010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3663761"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1452
                     /locus_tag="VLI90_14325"
     CDS             37..1452
                     /locus_tag="VLI90_14325"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSV15432.1"
                     /translation="MNGNGDASTGATTATATAATAPGVTRIVILGGGFGGVYTARHLQ
                     KLAGSWRPIEITLVNRDNYFLMTPLLFEAGSGVLEPRHSVNPIRPLLQRTRFVEAEIQ
                     GVDFDRRVVIAAPTPDEVYEIPYDQLVIAVGGVTNTSLVPGSEHAMTFKTLGDAIFIR
                     NHTIDLFERADVERDPRRKAAMLSFVVVGAGLVGTELVGELTVFVGHVADSYRNIDRK
                     DIRIELIEAGKKIVPEMDTELTDYAAKVLERRGVRIRVNTKVDRIEPHQIHLKSGDGQ
                     PTEAIAADTIVVATGVKPSPLVDGLQLEKDRKGRIIVDGTMLAKGRTNVWALGDCASI
                     PNPNDPDGRPYPQLAQHALREAKVLARNIMHSLNNEPLEPFIYSNKGVLAALGHYRGV
                     GRVYKLRIYGFLAWWVWRTYYVMQMPQWPRRIRIIIDWTVALMFKNDIAKLDLFGLEH
                     PLTPRKLQQPADRRVLKEEIG"
     gene            complement(1588..1761)
                     /locus_tag="VLI90_14330"
     CDS             complement(1588..1761)
                     /locus_tag="VLI90_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15433.1"
                     /translation="MLDLRSLVQIKLSAFCDKDRVHVRDMIDVGLIDRAWLTTLPPDL
                     AQRLQSLLDTPGG"
     gene            2050..2718
                     /locus_tag="VLI90_14335"
     CDS             2050..2718
                     /locus_tag="VLI90_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15434.1"
                     /translation="MLHWLLHTDAGLFTRIAIGAAIFGVLAFVDFRKNGRSATRWREY
                     GVLLIAVAAAVVYGAINDQITAMISWEYFYYGKELHRVLGDATPPEMSRLRWEAAKVG
                     VRATWTAGLIFGVVLLIANNPLRGLPRLRNRELLRILPVILLFAIVCGAIGGVLGYYG
                     ALRHMAADFEDMWRTNYFRPRRFMCTWGVHLGGYVGGFLGAASAAIWIVRTRVRRGSR
                     RRGS"
     gene            complement(2789..>4196)
                     /locus_tag="VLI90_14340"
     CDS             complement(2789..>4196)
                     /locus_tag="VLI90_14340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV15435.1"
                     /translation="DLRDHPDWEIVAKADLPPVTGGMLAAPLTGRDGTNLGVIYLSDR
                     FDGEPFTHDDEAILVQLAQMGSIAIENTIYAQEREANRIKDEFLATLSHELRTPLNAI
                     LGWTQLLKMEQMPAEVAHGMEVIDRNARAQTKLIEDLLDVSRITSGKLRLNMKRSTFG
                     PIVQAAVDAARPAAEAKQIHLDVRLADDEIPVSLDPDRIQQVVWNLLTNAVKFTPTGG
                     RVTVALQRTGDPGAERVTLRVTDTGQGIDGKFLPYVFDRFRQADSTSTRAHGGLGIGL
                     TIVRHIVEQHGGTVAADSAGPGRGAVFTVELPAAGQVPATLTTTADAPSSQSSQKQSI
                     SGLRILLVDDDPDARDVIAATLRRAGGEVTTVGSALEALDTLPGAKPDVLVSDIAMPD
                     QDGYALIRRIRQLPPEDGGEVPAIALTAYAREEDRARSLAAGFQVHLAKPVDPMDLLG
                     RITQLAAQAAGQPVLRLA"
BASE COUNT          696 a         1234 c         1506 g          760 t
ORIGIN      
        1 taatgtcgtg cagcgaacga tctcggaagg acacacatga acggcaacgg agacgcctca
       61 actggcgcaa cgacggcgac ggcgacggcg gcgacggcgc cgggggtcac gcgcatcgtc
      121 atcctcggcg gtgggttcgg cggggtgtac accgctcggc atctgcagaa gctcgccggc
      181 tcgtggcggc cgatcgagat cacgctggtc aatcgcgaca actatttcct gatgacgccg
      241 ctgttgttcg aagcgggatc tggcgtgctc gagccgcggc acagcgtgaa cccgatccgc
      301 ccgctgctgc agcgcacgcg gtttgtcgag gcggagatcc agggcgttga tttcgatcgc
      361 cgtgtcgtca tcgccgcgcc gacgccggac gaggtatatg agattccgta cgatcaactg
      421 gtgatcgccg tgggcggcgt caccaacact tcgctcgtgc ccggcagcga gcacgcgatg
      481 acgtttaaga cgctcgggga cgcgatcttc atccgcaatc acacgattga tttgttcgaa
      541 cgcgccgacg tcgagcgcga tccgcggcgc aaggcggcga tgctcagctt tgtcgtcgtc
      601 ggcgcagggc tggtgggcac ggaactggtc ggcgagctga ccgtcttcgt cggccatgtc
      661 gccgactcgt acagaaacat cgaccgcaag gacatccgca tcgagttgat cgaggcgggc
      721 aagaagatcg tgccggagat ggacacggag ctgaccgatt acgctgcgaa ggtgctggaa
      781 cgccgcggcg tgcgcattcg cgtgaacacc aaagtcgatc gaatcgagcc gcatcagatc
      841 cacctcaaat cgggtgacgg ccagccgacg gaggcgatcg ccgccgacac gatcgtcgtc
      901 gccaccggcg tgaaaccgag cccgctggtc gatgggttac aactggagaa ggaccgcaag
      961 ggccgcatca tcgtggacgg cacgatgctg gcgaagggcc gcacgaatgt gtgggcgctg
     1021 ggcgactgcg cttcgattcc caaccccaac gaccccgatg gccggccata cccgcagctg
     1081 gcgcagcacg cgctgcgcga ggcaaaagtg ctggcgcgga acatcatgca ttcgctgaac
     1141 aacgagccgc tcgagccgtt catctattcc aacaagggcg tgctggccgc gctcggtcac
     1201 taccgcggcg tcggtcgcgt gtacaagctc cgcatctacg gcttcctcgc gtggtgggtc
     1261 tggcgcacgt actacgtcat gcaaatgccc caatggcccc ggcggatccg catcatcatc
     1321 gattggacgg tggcgctgat gttcaagaac gacatcgcta agctggacct gttcgggttg
     1381 gaacacccgc tgacgccaag gaagctgcag caaccagcgg atcggcgggt gctgaaagag
     1441 gagattgggt gaggagttga tcctgagcgc aggacgtact cgtgcgtagt cgaaggatct
     1501 gcccggtcgc agtgccagcc gcttgcggct tagcgtctgt tttctttaag ggggaaagga
     1561 cgccaagccg caagcggcga tggctcatca tccacccggc gtatcgagca gtgactgcaa
     1621 tcgctgcgcg agatcgggcg ggagtgttgt gagccacgcg cggtcgatca atccgacgtc
     1681 gatcatgtcg cgcacgtgga cgcggtcctt gtcgcaaaat gcggacagct tgatctggac
     1741 gagcgagcgg aggtccaaca cgcgatatga ttcggcgggc tccgatgcgg tgacatcggg
     1801 attcgcgatg ctctcgtgcg ggcggacctt ctcattggca aagatgatgt gtaccgcgtc
     1861 gcgcgcgcgg cgttaccgcc cgccacggca tagggaacgc cggccgcttc cagaatggcg
     1921 gtggcgcgaa gcaggcgttg gcgaacgtct tccacagcgc ttaccacccg atccagtgag
     1981 aatggtccga ggacaacggg cacaagttac aattatacga tacgcgcgcc cgtgctacat
     2041 tggccgggaa tgctccactg gttgctgcac accgacgcag ggctcttcac gcgaatcgcg
     2101 atcggcgctg cgatcttcgg ggtgctggcg tttgttgatt ttcgaaagaa tggccgctcc
     2161 gccacgcggt ggcgggagta tggcgtgctg ctgatcgcgg ttgcggcggc tgttgtttac
     2221 ggggcgatca acgatcagat cacggcgatg atttcctggg aatactttta ctacggcaag
     2281 gaactccatc gcgtgctggg cgacgcgacg ccgccggaga tgtcgcgatt gcgctgggag
     2341 gcggcgaagg tgggcgtgcg ggcgacttgg acggcggggt tgatcttcgg cgtggtgctg
     2401 ttgattgcga acaatccgct gcgcgggttg ccgcggctgc gaaaccgcga gttgcttcgc
     2461 atattgccgg tcatcctgtt gttcgcgatc gtgtgcggcg cgatcggtgg ggtgctcgga
     2521 tactacggcg cgctgcggca catggcggcc gacttcgaag acatgtggcg gaccaactat
     2581 ttccgtccgc ggcggttcat gtgcacgtgg ggcgttcacc tcggcgggta tgtcggcggg
     2641 ttccttggcg ccgcgtcggc ggcgatatgg atcgtgcgca cgcgcgtgcg gcggggcagc
     2701 cgcagacggg gatcttaggt tgatcgactg ccagatcgct cgcgcgggct ggaggcccgt
     2761 gtcgcggtga tcgttcgaac atctgatcct acgccaaccg cagcacgggt tggccggcgg
     2821 cttgggcggc gagttgggtg attcttccca atagatccat cggatcgacg ggcttggcga
     2881 gatggacctg gaaaccggcg gcgagggagc gggcgcggtc ttcttcgcgc gcgtaggcgg
     2941 tcagcgcgat ggcggggact tcgccgccgt cttcgggggg gagctggcgg atgcggcgga
     3001 tcagggcgta gccgtcctgg tcgggcatcg cgatatcact gaccagcaca tccggcttgg
     3061 cgccgggcag cgtgtcgagc gcttccagcg cggagccgac cgtcgtcact tcaccaccag
     3121 cgcggcggag ggtggcggcg atcacgtcgc gcgcgtcggg gtcatcgtcc accagcagga
     3181 tgcgcaggcc cgagatcgat tgcttctgcg acgattgcga tgaaggagcg tcggcggtgg
     3241 tcgtgagcgt cgcggggact tgcccggcgg cggggagctc gacggtgaag acggcgccgc
     3301 ggccggggcc ggcgctgtcg gcggcgacgg tgccgccgtg ttgttcgacg atgtggcgga
     3361 cgatcgtgag gccgatgccc aggccgccgt gggcgcgggt gctggtgctg tcggcctggc
     3421 ggaagcggtc gaagacgtag gggaggaact tgccgtcgat gccctgaccg gtgtcggtca
     3481 cgcggagcgt gacgcgttcg gcgccggggt cgccggtgcg ctgcaacgcg accgtcacgc
     3541 gcccaccggt tggcgtgaac ttcaccgcgt tggtcagcag gttccagacg acttgctgga
     3601 tgcggtcggg atcgaggctg acgggaatct cgtcgtcggc cagccgaacg tcgaggtgga
     3661 tctgcttggc ctcggcggcg gggcgggcgg catcgacggc ggcctgcacg atcgggccga
     3721 acgtcgagcg cttcatgttc aggcgaagct tacccgacgt gatgcgcgac acgtcgagca
     3781 ggtcttcgat cagcttggtc tgcgcgcggg cgttgcggtc gatcacctcc atgccgtgcg
     3841 cgacttccgc gggcatctgt tccatcttca gcagctgcgt ccagccgagg atcgcgttca
     3901 gcggcgtgcg cagctcgtgg ctgagcgttg cgaggaattc gtccttgatg cggttcgctt
     3961 cgcgctcctg cgcgtagatc gtgttctcga tggcgatcga tcccatctgc gccagctgaa
     4021 cgaggatcgc ctcgtcgtcg tgggtgaacg gctcgccgtc gaagcggtcg gagaggtaga
     4081 tgacgccgag gttcgtgccg tcgcgaccgg tcagcggggc ggcgagcatg ccgccggtga
     4141 cgggcggcag gtcagccttg gcgacgatct cccaatcggg atgatcgcgc aggtcc
//
LOCUS       DATIMH010000828         7237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3947096, whole genome shotgun sequence.
ACCESSION   DATIMH010000828 DATIMH010000000
VERSION     DATIMH010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3947096"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VLI90_14345"
     CDS             <1..621
                     /locus_tag="VLI90_14345"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HSV15436.1"
                     /translation="EYRERGPELRRLYKLAVVGVLVVYVQLILGATMRHYQAGLAIPD
                     LPLAYGHLLPPMSQAQLDAANHQRVWNLNLEPVTLTQVWLHFAHRVGAIVVTIALVTL
                     IGVILLRHRRTLLIPAVTLSLLLLGQLTLGVLTVYFRKPADIASLHVAVGALVLVTTF
                     SIAVAAMRLYSLRSSTATPGSEVLRRPGSVPVEEHAGRREYAPNPA"
     gene            650..1603
                     /gene="cyoE"
                     /locus_tag="VLI90_14350"
     CDS             650..1603
                     /gene="cyoE"
                     /locus_tag="VLI90_14350"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HSV15437.1"
                     /translation="MKPASEISDLPDGFAVATAAMDADVRTVAAPTEPSRVNDFYELT
                     KPRMNFLIVITTMVGYFMASRGDANWTRLLHTLLGTALTASGAGVLNQLIEREYDKLM
                     PRTRNRPLPAGRVAPAEAVCYGVALGVAGVTYLAILVNALTASLGALTLLSYLFIYTP
                     MKRRTTLNTVIGAVPGAIPPVMGWTAVHNVIQPEAIALFAILFFWQMPHFLAIAILYR
                     NDYKAGGFKMLPVVDEDLIITGRQIVLYAVALVPVTLMPTVIGMAGSAYFTAAMLLGL
                     AFLSFAISCAATRTRSDARKLFFASIIYLPLLLAVMMLNKM"
     gene            1721..2485
                     /locus_tag="VLI90_14355"
     CDS             1721..2485
                     /locus_tag="VLI90_14355"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSV15438.1"
                     /translation="MPRKKLARFAILGVVFLIIAAVITECDEVPDTGTAKPAPRTAVQ
                     PGTGVVRGRATFNGPRPEPKVVGGECCPGAAPAVDESVIVSDDGGLKNVVVYIKDGPL
                     VRKPPATQPAAIAVLDQKDCRYVPHVIAMQTGEPLDVTSHDPTMHNVHVQSVVNPAAN
                     FSETAVGASHQITFAAPEFIRVKCDVHPWMTAYIAVFDHNCFAVTGDDGRFQIDSLPP
                     GIYTLVARHERYGSLERQFTVTGDQPVNVQFDFRGP"
     gene            2618..3499
                     /locus_tag="VLI90_14360"
     CDS             2618..3499
                     /locus_tag="VLI90_14360"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HSV15439.1"
                     /translation="MSESVLDHGYTPEEHAPDPVISHGDPTPPGKVGIWMFLASEIMF
                     FMGILGSYIILRSNQHELFARHAEALSKPLAGLNTLVLIFSSLTMALSVDASQRGDRK
                     KVIIGLFITLMCACGFLAIKTVEYTSKYYHHTIVARDASDGRTYVYDGHEKAHDDTSI
                     TLQADRKELVPGVPFDIHLVSEMDVKGEPEGTEPHVAEARPERTAGEPMYKINRADIA
                     QDTRYGPSKNLFYGCYFTLTGIHGLHVIGGIIPIFFLLVQAARGKLFPAQTEYIGLYW
                     HFVDLVWIFLFPLLYLI"
     gene            3655..4062
                     /locus_tag="VLI90_14365"
     CDS             3655..4062
                     /locus_tag="VLI90_14365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HSV15440.1"
                     /translation="MNTVADRIPNDVPHGHTESTPHHQVNYVMIFVMLVILTAATFGV
                     SFYRFSSEFINVLLAMIVATTKASLVAMFFMHLKFEGKLVRALMIVPLCLCVILVTAL
                     IPDIFMTDPVRNFASSSLHLFNPITALFHGFGK"
     gene            4175..5152
                     /locus_tag="VLI90_14370"
     CDS             4175..5152
                     /locus_tag="VLI90_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSV15441.1"
                     /translation="MSDPIVEINHLTHAYGERVAVNDLSLTVSRGEIFGFLGPNGSGK
                     TTLFRILSTLIPAPPGAVKMFGLDAAEAMNAIRNRIGVVFQSPSLDKQLTAAENLRHH
                     GHLYGIHGPELDERMRTLLQRVDLLDRQHERVEGFSGGMRRRVELAKGLLNRPEILLL
                     DEPSTGLDPGARIDLWRMLRDIQASGVTILLTTHLMDEADRCSRLAIMTAGKLVACDT
                     PTAMKERIGGDVITITSNDPERLRSTLRERLNVEAETVDAALRLERPRGHEFVPQLIE
                     AAPGLVESVSVGKPTLEDVFLHLTGKRFRDEEANADSAAQHRTPRRRAG"
     gene            complement(5208..5549)
                     /locus_tag="VLI90_14375"
     CDS             complement(5208..5549)
                     /locus_tag="VLI90_14375"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSV15442.1"
                     /translation="MRKHGPGASDQDLEQLTALFRLLSDKTRLNILILLANGERNVTS
                     LCEELQLPQPTVSHHLGLLRMNNVIGNRRAGKQVFYGLNGRVDAGESSLVIAAQNFDI
                     RIGERAASTAS"
     gene            5845..>7237
                     /locus_tag="VLI90_14380"
     CDS             5845..>7237
                     /locus_tag="VLI90_14380"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HSV15443.1"
                     /translation="MHRPVALSLLSGAGLLVPTAAWAGGTSHPAHPPLSPLFLDRMTV
                     TAYTLGEDPIPGFIPQVIMGVTNEQDSDTSGSGVEGFGIASSTPGGSSLPIGGSPLYV
                     VATLDSGSQSHIISYNDSIPPGFDIQGAGLEGAGSSTIVGISGSETVSITDALGVYAT
                     DLSNGSVSNNAINVTPGTLVGHYNVPVLTGNQGDGLPNVVGSPILAQYRVTIQNSQPR
                     HLTVGGTTYKSPRIGFSSFPSTGGVPTNYSKLTLSALDPIFGGAETQPTPYYINFASN
                     GPNDPDTPGAWASLETNGGVGLSRSGQTFGNQSFLLDTGAQLSVISNDTASQVGIFNA
                     SPANADFTVDVQGVGGITTVPGYYLDSLNLTTQGGVITWKHVPVLVVDLPNPDGSSTP
                     LPGLLGTNLFSDRDLVLNTDVSHTGQTFIAIGPQLAWTTKGGGTWSTASNWANMPAAP
                     NGIDMQANFYDSIT"
BASE COUNT         1380 a         2255 c         2192 g         1410 t
ORIGIN      
        1 gagtaccggg agaggggacc agagttgcgc cgactgtaca agttggcggt cgttggcgtt
       61 ctcgtcgtat acgttcagct gatcctcggc gcgacgatgc ggcattatca agcaggatta
      121 gcgatccccg acctgccact tgcctacgga cacctgctcc cgccgatgtc gcaggcgcag
      181 ctcgatgccg ccaatcacca gcgcgtgtgg aacctgaacc tggagccggt cacgctcacg
      241 caggtctggc tgcacttcgc ccaccgcgtc ggggcgatcg tcgtcacgat cgcactcgtg
      301 acgctgatcg gggtcatcct gctccgccat cgccgcacgc tgcttatccc ggcggtgacc
      361 ctgagcctcc tgctcctcgg gcagctgaca ctcggcgtcc tcactgtcta cttccgaaaa
      421 ccggcagata tcgccagcct tcacgtggct gtgggggcct tggtgttggt cacaacgttc
      481 agcattgcgg tggcggcgat gaggctgtat tccttacggt cgtcgaccgc cacgccgggt
      541 tcggaggtac tccgacgacc aggatccgtg cccgtcgaag aacatgcggg tcgtcgcgag
      601 tacgctccga acccggcgtg aacaacaaca ggaaagaccc tcagtcgaaa tgaagcccgc
      661 atctgaaatt tccgatcttc ccgacggctt cgccgtcgcc accgcggcga tggacgccga
      721 tgttcgaacc gttgccgcgc caaccgagcc ttcccgcgtc aacgactttt atgagctgac
      781 caagccgcgg atgaacttcc tcatcgtgat cacgacgatg gttggttact tcatggcgtc
      841 gcgcggcgac gcgaactgga ctcgattgct gcacacgctg ctgggcaccg cgctgaccgc
      901 cagcggcgcg ggtgtgttga accagctgat cgagcgggaa tacgacaagc tgatgccgcg
      961 gacgcgcaac cgcccgctgc ccgccggtcg cgtcgcgccc gccgaagccg tctgctacgg
     1021 cgtcgcgctc ggcgtcgccg gtgtaactta tctagcaatt ctagtaaacg cactcaccgc
     1081 gtcgctcggg gcgctgacgc tgctcagtta cctcttcatc tacacgccga tgaagcggcg
     1141 aacgacgctg aacaccgtca tcggcgccgt ccccggcgcc atcccgcccg tcatgggctg
     1201 gaccgcggtg cataacgtca tccaacccga agcgatcgcg ctgttcgcga tcttgttctt
     1261 ctggcagatg ccgcacttcc tggcgatcgc gatcctctat cgcaacgact acaaggccgg
     1321 cggctttaag atgctgccgg tcgttgacga agatctgatc atcaccggcc ggcagatcgt
     1381 gctgtatgcg gttgcgctgg tgccggtcac gctgatgccg accgtgatcg gcatggccgg
     1441 cagcgcctac ttcaccgcgg ccatgctgct ggggctggcg ttcctgtcgt tcgcgatctc
     1501 ctgcgcggcg acgcgcacgc gcagcgacgc gcgaaagctg ttcttcgcgt cgatcattta
     1561 tctcccactg ctgctggcgg tgatgatgtt gaacaagatg tgacacgggc gcgcgcagtc
     1621 cttaatagcc ggggccggaa ggcccggccc gttcgaactg aaaaacaccg gggctgccgc
     1681 ccccggcttt gacggagacg gagcaagaaa cgagacgtct gtgccgcgga aaaaattagc
     1741 gcgattcgca atcctcggcg tggtgttttt gatcatcgcg gcggtcatca ccgaatgtga
     1801 tgaagtcccc gacaccggga cggcgaagcc agcgccgcgg acggccgtgc aaccgggaac
     1861 cggcgtggtc cgcggtcgcg cgacgttcaa cggcccccgg ccggagccaa aggtggtcgg
     1921 cggcgaatgc tgccccggcg cggcgccggc tgtcgatgaa tcggtgatcg tcagcgacga
     1981 cggcggtttg aagaacgtcg tcgtgtacat caaggacgga ccgctggtgc ggaagccgcc
     2041 ggcgacgcag ccggcggcga ttgcggtgct ggaccagaaa gattgccgat acgtgccgca
     2101 cgtgattgcg atgcagacag gcgagccgct ggacgtgacc agccacgacc cgacgatgca
     2161 caacgttcac gtgcagtcgg tcgtgaaccc ggcggcgaac ttttcggaga cggcggtggg
     2221 cgcgagccac caaatcacat tcgccgctcc ggagttcatc cgcgtcaagt gcgacgtgca
     2281 cccgtggatg acggcgtaca ttgcggtgtt cgatcacaat tgcttcgccg tgaccggcga
     2341 cgatgggcgt tttcaaatcg acagcttgcc gcccggcata tatacgctgg tcgcgcggca
     2401 cgagcggtat gggtcgttgg agcggcagtt caccgtaacc ggcgatcagc cggtgaatgt
     2461 tcagttcgat tttcgcggcc cctgagttga aggcgaagag acaaggggac aaggagacac
     2521 gcggaacaag cagagacagg atcagacgtg ttcgtctttc cccttgtctc cttgtccccc
     2581 ggtctccttg tctgtttcat ccgaaacgga gagcactgtg tccgaatccg tcctagacca
     2641 cggctacacc cccgaagaac acgcgcccga tccggtcatc agccacggcg atcccacgcc
     2701 gcccggcaag gtcggcatct ggatgttcct ggccagcgag atcatgttct tcatgggcat
     2761 cctcgggtcg tacatcatcc tgcgctcgaa ccagcacgag ctgttcgccc ggcacgccga
     2821 ggcgctgagc aaaccgctcg cggggctaaa cacgctcgtg ctgatcttca gcagcttgac
     2881 gatggcgctg tcggtcgatg cctcacagcg cggcgatcgc aagaaggtca tcatcggcct
     2941 gttcatcacg ctgatgtgcg cgtgcggatt tctggcaatc aagacggtcg agtacaccag
     3001 caagtattac caccacacga tcgttgcgcg cgacgcgagc gacgggcgga cgtatgtgta
     3061 tgacggccac gagaaggcgc acgacgacac gagcatcacg ctgcaggcgg accgcaagga
     3121 gctggtgccg ggtgtgccgt tcgatattca cctcgtcagt gagatggacg tgaagggtga
     3181 gcctgaaggc accgagccgc acgtggccga agcccggccc gagcgcaccg cgggcgagcc
     3241 gatgtacaag atcaaccgcg ccgacatcgc gcaggacacg cgttacggcc cgtcgaagaa
     3301 cctgttctac ggctgctatt tcacgctaac cggcatccac ggactgcacg tgatcggcgg
     3361 catcatcccg atcttcttcc tactggtgca ggccgcccgc ggcaagctgt tccccgcgca
     3421 gacggaatac atcgggctgt attggcactt cgtggacctc gtgtggattt tcctgttccc
     3481 gttgctgtat ttgatctaac ctgggatcag agatgatgaa cgcagagacg cagaggccgc
     3541 agaggaggaa cagagacaga gggagagaga tctgaagctc gcaatgatct cgtgtcttaa
     3601 ctctgcggcc tctgcgtctc tgcgttcaat gatcttctat caggagctcg cgatatgaac
     3661 accgtcgccg atcgcattcc aaatgacgtc ccgcacggcc acaccgagtc cacgccgcat
     3721 catcaggtga attacgtgat gatcttcgtg atgctggtga tcctgacggc cgccacgttc
     3781 ggcgtgtcgt tctatcgctt cagcagcgaa ttcatcaacg tgctgctggc gatgatcgtc
     3841 gcgacgacca aggcgtcgct cgtggcgatg ttcttcatgc acctgaagtt cgaaggcaag
     3901 ctggttcgcg cgctgatgat cgtgccgctg tgcctgtgcg tgatcctggt gacggcgttg
     3961 atccccgaca ttttcatgac cgaccccgtc cgcaactttg ccagctcgtc gctgcacctg
     4021 ttcaacccga tcacggcgtt gtttcacgga tttggaaaat gattaaccac agatgcacac
     4081 ggatgcacac agataggaga gcgtcgcccc ggtctgtggt tccatttgat ttgtgattct
     4141 ggcacgggcg agacgcccgt gctactgttt tgcgatgtct gatcccatcg ttgaaatcaa
     4201 ccacctcact cacgcctacg gcgagcgcgt ggcggtgaac gatctttctc tcaccgtcag
     4261 ccgcggggag atcttcggtt tcctcggccc caacggcagc gggaaaacta cgctcttccg
     4321 catcctctcc acgctcatcc cggcgccacc cggcgcggtg aagatgttcg gcctcgacgc
     4381 ggcggaggcg atgaacgcca tccgcaatcg catcggcgtt gtgttccaat ctcccagcct
     4441 cgataagcaa ctgacggcgg cggagaacct gcgtcatcac ggacacctct acggcatcca
     4501 cggcccggag cttgacgagc gcatgcgtac gttgcttcag cgcgtcgatc tgctcgaccg
     4561 gcagcacgag cgcgtcgaag gtttttccgg cggtatgcgc cgccgcgtcg agttggcgaa
     4621 aggtctgctc aatcgcccgg agatcctcct gcttgacgaa cccagcaccg gcctcgatcc
     4681 cggcgcccgc atcgacctgt ggcggatgct ccgcgacatc caggccagcg gcgtgaccat
     4741 cctgctgacg acgcacctga tggacgaagc cgaccgctgt tcgcggctgg ccatcatgac
     4801 cgctggcaaa ctggtcgcct gcgacacgcc aacggcgatg aaggaacgta tcggcggcga
     4861 tgtgatcacc atcacgtcga acgatcccga gcggcttcga tcgacgctgc gcgagcggct
     4921 gaatgtggaa gcggaaacgg tggacgccgc gctacgcctg gagcgcccgc ggggacatga
     4981 attcgtgccg caattgatcg aggcggcgcc tgggctggtg gagtcggtgt cggtcggaaa
     5041 accgacgttg gaagacgttt tcctgcacct gaccggcaag cggttccgtg atgaggaagc
     5101 gaatgccgat tcggctgcgc agcaccgaac cccccggcgg cgggccggat agggtaaggt
     5161 ttatcttata ggctgcggaa gaattgaggg ggggcaatcg gcggtcgtta cgacgctgtc
     5221 gaggccgcac gttccccgat gcggatgtcg aagttctggg cggcgatgac cagcgacgac
     5281 tccccggcgt cgacccgacc gttaagccca tagaacacct gcttgcccgc ccggcggttg
     5341 ccgatgacgt tgttcatccg cagcagtccc agatgatggc tcaccgtagg ttgtggcagc
     5401 tgcagctctt cgcacaagct agtgacgttc cgctctccat tggccaacag aatcaggatg
     5461 tttaatcgcg tcttatccga cagcagtcgg aacaacgcgg ttaactgctc cagatcctgg
     5521 tcactcgccc ctggtccatg tttgcgcatc aagatgtctc cctcgataat accccgaccc
     5581 gggcttatcc gtttaaccgg ttggtctacc cttggactat aggtttaccg cacagcaacc
     5641 gtcaaagtat gacggaaaat ggaaaaagtg cggcgaattg ggaaattcta cccgtttttg
     5701 cagttgaatc ggttcaaaat tgacggaaac attacagaaa ttgttttttg cgcccggtgg
     5761 ttgacgccgt gggcaggtgg gggacatgcc gtatactttt gaacaagcgg gggtggcgga
     5821 acgtgatgct attggaggaa cgagatgcat cgtccggtcg cgttgtcgtt gttgtcgggg
     5881 gctggactgc tggtgccaac cgccgcgtgg gcgggcggca cttcgcaccc ggcgcatccg
     5941 ccgctgtcac ccctgttcct ggaccggatg acggtcaccg cctacaccct gggcgaagat
     6001 cccatccccg gtttcattcc ccaggtgatc atgggtgtga cgaacgaaca ggactcggat
     6061 acctccggca gcggggtcga gggattcggc atcgcaagtt ccacgccggg gggttcaagt
     6121 ctgccaattg gcggttcgcc gttgtacgtc gtcgcgacgc tcgacagtgg atcgcagtct
     6181 cacatcatca gctacaacga ttcgatccca ccaggctttg acatccaagg cgccggcctg
     6241 gaaggcgcag gttcaagcac gatcgtcggc atcagcggtt cggaaactgt gtctatcact
     6301 gatgcgctgg gcgtgtatgc caccgatttg tccaatggaa gcgtttcgaa caacgcgatc
     6361 aacgttacgc ccggcacgtt ggtcgggcat tacaatgtgc cggtcttgac cggcaatcag
     6421 ggcgatgggc tgccgaacgt ggtcggttcc ccgatcctgg cccagtaccg cgtgacgatc
     6481 cagaattcgc agccgcgtca cctgaccgta ggcggcacga cgtacaaatc gccgcgcatt
     6541 ggcttcagca gtttccccag caccgggggt gtgccgacga attattcaaa gctcactttg
     6601 tcggccctgg acccgatctt tggtggcgcc gaaacgcaac cgacgccata ttacatcaac
     6661 tttgcttcaa acggcccgaa cgatcccgat acgcccggcg catgggcctc cctggagacc
     6721 aatggcgggg tggggttgtc gcgcagcggt cagacgttcg gcaatcaatc cttcctgctc
     6781 gacaccggcg cgcagctcag cgtcatcagc aacgacaccg cctcccaggt cggcatcttc
     6841 aacgcatcgc cggccaatgc ggacttcacc gtcgatgtcc agggtgtcgg cggcatcact
     6901 acggtgccgg gttactacct cgattccttg aacctcacca cccagggcgg cgtgatcacc
     6961 tggaaacatg tgccggtgct ggtggttgat ctaccgaacc cggatggctc aagcacccca
     7021 ctgcctggct tgctgggaac aaatctcttt agcgatcgcg acttggttct caacaccgac
     7081 gtcagtcaca cgggccaaac gttcattgct atcggcccac aactggcttg gacgaccaag
     7141 ggcggcggca cgtggagcac cgccagcaac tgggccaaca tgccggccgc gcccaacggc
     7201 atcgacatgc aggcgaattt ttacgactcc atcaccg
//
LOCUS       DATIMH010000829         4113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3373039, whole genome shotgun sequence.
ACCESSION   DATIMH010000829 DATIMH010000000
VERSION     DATIMH010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3373039"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VLI90_14385"
     CDS             complement(<1..541)
                     /locus_tag="VLI90_14385"
                     /inference="COORDINATES: protein motif:HMM:NF020844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016837 - carbon-oxygen lyase activity,
                     acting on polysaccharides [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase B N-terminal
                     domain-containing protein"
                     /protein_id="HSV15444.1"
                     /translation="MLEDRTLFAFGLTTSTNLYTVDSGSGLVFSVQRTHPSGSVGDLT
                     SAKLNGTELEAPFANSSRYSHYESGLSSATVVTATVDPNGQWILIACDDTAAGGVGVI
                     QYYIGRKGFNNIYVATYAPGPGSPSPGEMRFIDYTNPSVLTNIPAPSNNSGSTGAIES
                     SDVFGHADGTTTSKYYGEYR"
     gene            complement(846..>4113)
                     /locus_tag="VLI90_14390"
     CDS             complement(846..>4113)
                     /locus_tag="VLI90_14390"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV15445.1"
                     /translation="RPALASDPTGDMVLTSSAGADENYAIHAERFAAFTNAASITGQL
                     WRDSNGNGVRDSGETAPGTSCTVNLFNNFMLMSSVTTTLNFSFSGVMPGESLYVQLSP
                     PTGFFLTLYNAGGSAPVSSQIDPATGRSATLTLAADQSLSDFNIGLIPAATIVGSVFS
                     DLLSDGTLSGGDAGLPGKTVYLDSNGNGILDAGESSQLTDFKGGFSFSLPPGNYTLRV
                     VDDGTNVDPAPVETASVVPAQRLTVNIPIHSLKAVPAPAAAGAAGAVGPGSAVDVATD
                     AAGDSVIAYKQTSGTTTNIFAQRFSSTGAAVAGPFRVNTSNLASDEAQVGIANDGSFV
                     VAWRGGNVVRAQRYAADGTPVGGEMVVETPPAGDSVASMDLSMNAGGAFVVAWSMISS
                     PSGVLDTFVRPYDAAGNAVTDVVQASMTDAAIDTHPSVAMGDDGSFFVLWQALDAAKQ
                     NQMVFGRRFDATGTPLAPQLAINTTTTGEQWYPSVSVNSSGYSAVVWLANAPPGVAGE
                     GVYIQRYDAIGNAIGREMLLSIDGGLINHITLGEQGDMLVSWCPDTTGLQATYLDAAG
                     TPHSFVPINDNIVHGAFGADMDAAGNVTFGYDTSGTVNVRRFVSRPVVLATNFQLTAT
                     PVMNFVLRLSSDVAALAGANDVTILNNNTSTAIPASAMAITAASDNTLTITFPGYANG
                     ELPSGSYTFTLPASKVVNTAGHTMSADFVSRFLWVAGTDAADTIIVRRASDKSTIQVF
                     DNIPTTGSPSYTAPALTLGQFRIDGGGGDDTVTFDFTNGTPFTTFGFSYTGGSGSNSL
                     LLTAATGVHSVVAAAASGITFDSTTVPFAVQNLLVDPAGGSDFLGVAGMIVTVPANAA
                     GSGIVARTFLRLSVSAGGIVRFADAASPLDRTVAVVTSTSLPISLNQGSIDLGGNDMI
                     IRSGNLATIASLLQNGLNMASGGHWNGIGIQSSTAAAHVNTALGVLQNVTSGSLFDRQ
                     TLAANDVTIKYTLNADADLNGGVDVGDLGKLATNYGVSTGATWSQGDFNYDGKVDVGD
                     LGALAINYGQSLGGGAASAESDTAISVGSAALPPTAFTNFTWLRIDARREFLRLVGLE
                     "
BASE COUNT          766 a         1214 c         1360 g          773 t
ORIGIN      
        1 cgcgatactc gccgtagtac tttgacgtgg tggttccgtc ggcgtggccg aagacgtcag
       61 agctttcgat tgccccggtg ctgccgctgt tgttcgacgg cgcggggatg ttcgtcagca
      121 ccgaaggatt cgtgtagtcg atgaagcgca tttcgcccgg cgacggtgaa cccggccccg
      181 gcgcgtaggt tgccacgtaa atgttgttga atcccttgcg gccgatgtag tactgaatga
      241 cgccaacacc acccgcggcg gtgtcgtcgc aggcaatgag aatccactgc ccattcgggt
      301 caacggtggc cgtgacaacg gtggcgctgc tgaggcccga ttcgtaatgc gaatagcggc
      361 tggagttggc gaacggcgct tcaagttcgg tgccgttcag cttcgcggaa gtcaggtccc
      421 ccaccgaccc actggggtgc gtgcgctgga ccgagaagac cagccccgag ccggaatcga
      481 cggtgtacag gttggtgctg gtggtgaggc cgaacgcgaa cagcgttcgg tcctcgagca
      541 tttcaacggc ggccaaagcg ctggaagcgc aagcccgctg cagaggcgac tggggcattg
      601 aagaaatcct ccgcaggacc aaccgcgtga cgcaaggcag gggacgagcc ggcgcggcgg
      661 ctcgcaaagt cagaaatatg caaggatcgg ttaagaattg caagataatt cccggaaaaa
      721 tctggtaatt accatcaatt atcaggagcg cagggcgggt agcggaatga actgtgccgg
      781 ggccgcgagg ccgacaggcg agcgcctttg ccacgaactt ccacccatcc gcgcaggatt
      841 ggtcgtcact ctagcccgac cagccgcaaa aattctcggc gggcgtcgat gcgtagccac
      901 gtgaaattag tgaacgcagt cggcggtagc gcggcgctgc cgacagaaat cgcagtgtcc
      961 gactccgcgc tggctgcgcc accaccaagg ctctggccgt agttgatcgc cagcgcgccg
     1021 aggtcgccga catccacttt gccgtcgtag ttgaagtcgc cttggctcca ggtcgcgccg
     1081 gtggacacgc cgtagttggt ggccagtttt ccgagatcgc caacgtcaac gccgccgttg
     1141 agatctgcgt ccgcattcaa tgtgtatttg atcgtgacat cattagcggc cagcgtctgg
     1201 cggtcgaaga ggctgcccga ggtcacgttc tgcagaacgc ctagcgccgt gttgacgtgt
     1261 gcggcggcgg tcgagctttg aattccaatg ccgttccagt gtccacccga agccatgttc
     1321 aacccgtttt ggaggaggga cgcgatcgtg gcgagattgc cgctgcgaat gatcatgtcg
     1381 ttgccgccca gatcgatgct tccttgattc agcgatatag gcagcgaggt ggatgtgaca
     1441 acagcaacgg ttcggtccag cggactggcc gcgtcggcaa agcgcacgat tcctccggcc
     1501 gaaacgctca accggagaaa ggtgcgcgcg acaatgccgc tacccgcggc gttcgcgggc
     1561 acggttacga tcattccggc cacgccgaga aaatcgcttc cgccggccgg gtccaccaac
     1621 agattctgca cggcgaaggg aacggtcgtg ctgtcaaacg tgattccact ggcagcggcg
     1681 gctacgacgg agtgcacgcc cgttgccgca gtgagtagga gcgaattgct gcccgatcca
     1741 ccggtgtagc tgaagccaaa tgtcgtgaac ggcgtgccgt tggtgaaatc gaaggtgact
     1801 gtgtcgtcac cacctccacc gtcgatgcgg aactggccca gggtcagggc cggggcggtg
     1861 tagcttggcg aaccggtcgt tgggatgttg tcgaatacct ggatcgtcga tttatccgag
     1921 gcgcggcgga cgatgatggt gtcggcggcg tctgtcccgg caacccagag gaatcgggag
     1981 acgaagtcgg cgctcatcgt gtgccccgcg gtattcacca ctttcgacgc cggcagcgtg
     2041 aaggtgtagc tgccggacgg caattcgccg ttggcataac ccgggaaggt gatcgtgagc
     2101 gtgttatcgc tggccgcggt gattgccatc gcgctggcgg gaatggccgt cgaggtgttg
     2161 ttgttgagga tcgtcacgtc gttggcgccc gcgagcgcgg caacgtcgct gctcaaccgc
     2221 agcacgaaat tcatcaccgg tgtggctgtc agttggaagt tggtcgcaag aaccaccggg
     2281 cgggaaacga agcggcggac gttcacggtt ccgctcgtgt cgtagccaaa ggtcacattg
     2341 ccggcggcgt ccatgtctgc gccgaatgcg ccgtggacga tgttgtcgtt gatcgggacg
     2401 aaactgtgcg gcgtgccggc ggcgtccagg taagttgctt gcaaaccggt tgtgtcggga
     2461 caccacgaga ccaacatgtc gccttgctcc cccaatgtga tgtggttgat gagacctcca
     2521 tcgatagaga ggagcatctc gcgaccgatt gcgttgccaa tggcgtcgta gcgctggatg
     2581 tacacgcctt cgccggcgac gcccggcggc gcattggcca gccagaccac ggcggaatat
     2641 ccgctggagt tgacgctgac cgacggatac cactgttcgc cggtggtcgt cgtgttgatc
     2701 gcgagctgag gggccagcgg cgttccggtc gcatcgaagc ggcggccgaa gaccatttgg
     2761 ttctgttttg cggcatccaa agcctgccat agcacgaaga aactcccgtc gtcacccatc
     2821 gcgaccgacg gatgggtgtc gatggccgcg tcggtcatcg acgcctgcac gacatcagtc
     2881 accgcgttcc ccgcggcgtc gtacggtcga acaaacgtgt cgagcacccc gcttggacta
     2941 gaaatcatgc tccacgcaac gacgaacgcg ccgccggcgt tcatcgatag atccatcgac
     3001 gcgaccgaat cgccggcagg cggtgtttcg accaccatct ctccgccgac cggtgttccg
     3061 tccgcagcgt atcgctgggc gcgaacgaca ttgccgccgc gccacgcgac gacgaagctg
     3121 ccgtcgttgg cgatgccgac ttgcgcttca tccgacgcca ggtttgacgt gttgacgcgg
     3181 aaggggcctg cgacggccgc gccggtggat gaaaagcgct gcgcgaagat attggtcgta
     3241 gtcccgctgg tctgtttgta cgcgattacg gaatcaccgg cggcgtcggt tgccacgtca
     3301 acggccgaac ctggcccgac ggcgccggcg gcacccgctg ccgccggcgc tggtaccgcc
     3361 ttcagcgagt ggatcggaat gttcaccgtc agacgctgcg ccggaacgac cgaggcggtt
     3421 tcgacaggcg caggatcaac attcgtgcca tcgtccacga cgcgcagcgt gtagttcccc
     3481 ggcggcagcg aaaaactgaa gccgcctttg aaatccgtca gctgcgacga ttcaccggcg
     3541 tccaggatcc cgttcccgtt gctgtcgaga tagaccgtct tgcccggcag gcccgcatcg
     3601 ccaccgctca acgtgccgtc ggagaggaga tcgctgaaga cgcttcccac gatggtggcg
     3661 gcgggaatga ggccgatgtt gaagtcgctc agcgattgat cggcagcgag agtcagcgtt
     3721 gcgcttcgtc ccgtggcagg gtcaatctgg ctgctcacgg gggccgaacc gccagcgttg
     3781 tagagcgtca ggaagaagcc cgttggcggc gagagttgga cgtagaggga ctcgcctggc
     3841 atcacgccgc tgaacgagaa gttcagcgtc gtcgttacgc tgctcatcaa catgaaatta
     3901 ttgaataggt tgacggtgca ggaagtgccg ggtgcggtct ctccgctgtc gcgcacgccg
     3961 ttgccgttgc tgtcacgcca cagctggccg gtgattgatg ccgcgttggt gaaggccgcg
     4021 aaacgctcgg catgaatggc gtaattctcg tctgcgccgg ccgagctggt gaggaccatg
     4081 tcgccggttg gatcgctcgc cagcgccggc cgg
//
LOCUS       DATIMH010000830         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1357243, whole genome shotgun sequence.
ACCESSION   DATIMH010000830 DATIMH010000000
VERSION     DATIMH010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357243"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VLI90_14395"
     CDS             <1..917
                     /locus_tag="VLI90_14395"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HSV15446.1"
                     /translation="VLWVESPVRISRHLRIRRWRYLTGDSGLLLGTTAFMMLVANGDY
                     LIVGYFCGSEAVGIFYFAFNLSMQTLALLMVNLANVLFPALSKLQAERPRQIEAYLRA
                     SRVFAAIAVPFCLMQAAGSDAGFRLIFKPIWYPSIPVLQILSVAMAIRTTAIPAQALT
                     AAQNRLRYSLNIAVAYSIVFLAIVALAARFGGPHAVIWVALAELVHFGITDPLYMYLL
                     MRMNGRGVREVFHVFKAPLIAGSIAALIAGGVSHVFPAGLSRSWQLPRLLAVVCVMVV
                     VYVPLLRLLSAELWQELVERVRALRAKA"
     gene            1032..2240
                     /locus_tag="VLI90_14400"
     CDS             1032..2240
                     /locus_tag="VLI90_14400"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV15447.1"
                     /translation="MRIFCAVRHSLDPRLYWSTLWSANFYPALRQMGHEIVESQTDLT
                     PGSHFLHIGGDYTPEQLRTRGQVTSKLLDELKAAHRERPIDLMLSYFYNSHFDPAGFD
                     EIHRMGIKTVNFYCNSMYQFELTSAIAPKVNYAWHAEKPARQRYLDVGANPVWVQMAA
                     DPDVYRPVPGLTRAPKSVFLGLRYADRAQWMAALIRADVPVDIYGPGWGNDPAPEPPA
                     TSGSAAGKPVQPPMSKAYAREVQRNFTQHGPIGGLKRTLVQWKMRNQQREMMPLFKPF
                     AKGAVPFTRIGEIFSSYEVVLNFINVWSDGRTGSELIPHVRLRDFEAPMSRTCFITGY
                     TDEITEFYEIGTEVDAYKSEAELVDKTKYYLSHPDAAERLRQAGYERALRDHTWVRRF
                     EQLFSMIGMS"
     gene            2237..3316
                     /locus_tag="VLI90_14405"
     CDS             2237..3316
                     /locus_tag="VLI90_14405"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSV15448.1"
                     /translation="MSTVSAGGKVLLVTGSSGLIGSEVVEYFASRGWTVHGVDNNQRA
                     DFFGPAGDTRWNQRRLLENYRSSFHHHELDIRNRDAVLKCVAEVKPNLLVHTAAQPSH
                     DLAASRPFDDFDVNAVGTLNMLEATRRHAPETVFVHMSTNKVYGDRPNSIRLKELDKR
                     WDYDDPAFADGIAEDFSIDQSKHSIFGASKVAGDVMVQEYGRYFGIRSCVLRGGCLTG
                     PKHSGVELHGFLSYLIKCNLEGKLYRVFGYKGKQVRDNIHSLDVARFIAAFYENPRIA
                     EVYNLGGGRANSCSIWEAFDRIASLSGKKMVHEYVDKNREGDHICYISNLSKMRRHYP
                     SWDISKSLDDIFREVYQSWRAGHQK"
BASE COUNT          646 a         1070 c         1161 g          683 t
ORIGIN      
        1 tcgtgctgtg ggtggaatcc ccggtgcgga tcagccggca tctgcgcatc cgccgatggc
       61 gctacctgac cggcgacagc gggctgctgc tgggcacgac cgcgtttatg atgctggtcg
      121 ccaacggcga ttacctgatc gtcggatatt tctgcggctc cgaagcggtc gggatttttt
      181 acttcgcgtt caacctgtcg atgcaaacgc tggcgctgct gatggtgaat ctggccaacg
      241 tgctgttccc cgcgctgagc aagctgcagg cggagcgccc gcggcagatc gaggcgtacc
      301 tgcgcgcatc gcgcgtgttc gccgccatcg cggtgccgtt ctgcttgatg caggcggccg
      361 gcagcgatgc ggggttcagg ctgatcttta aaccgatctg gtatccgtcg atcccggtgc
      421 tgcagatact gagcgtcgcg atggcgattc gcacgacggc catcccggcg caagcgctga
      481 cggcggcgca gaaccggctg cgctacagcc tgaacatcgc ggtcgcttat tcgatcgtct
      541 tcctggccat cgtcgcgctg gcggcgcggt ttggcgggcc gcacgcggtc atctgggttg
      601 cgctggcgga actggttcac ttcggcatca ccgatcccct gtacatgtac ctgctgatgc
      661 ggatgaacgg ccgcggcgtg cgcgaggtgt tccacgtgtt caaggccccg ctgattgccg
      721 ggtcgatcgc tgcgctgatc gccggcgggg tctcgcacgt gttcccggcg gggctgagcc
      781 ggtcctggca gcttccccgc ttgcttgccg tggtttgcgt gatggtggtg gtgtacgtac
      841 cgttgctgcg cctgctgtcg gcggagcttt ggcaggaact ggtggagcgc gtgcgggcgc
      901 tgcgcgccaa ggcatagcgt ctctcgccgc tagcggcttc gcagaccagc gcgggcggtt
      961 tcgccgcgac gttgcgcgac cctgcgggtt ggattgcgaa gccgcaagcg gctgaatcag
     1021 gttaatcatc tatgcgaatc ttctgcgccg tccgtcactc ccttgatccc aggctgtatt
     1081 ggagcaccct gtggagcgct aacttttatc cggcgcttcg gcagatgggg catgagatcg
     1141 tcgagtcgca gacggacctg acgccgggca gtcacttcct tcacatcggc ggggactaca
     1201 cgcccgagca attgcgcacg cgcggccagg tgacgtcgaa gctgctcgat gaactgaaag
     1261 ccgcccaccg cgagcgtccg atcgatctga tgctcagcta cttctacaac tcgcattttg
     1321 atcccgcggg gtttgacgag atccaccgga tggggatcaa gacagtgaac ttttactgca
     1381 acagcatgta tcagttcgag ctgacgtcgg cgatcgcccc gaaggtgaat tacgcgtggc
     1441 acgcggagaa gccggcgcgg cagcgatatc tggacgttgg cgccaatccc gtgtgggtgc
     1501 agatggcggc ggacccggat gtgtatcgcc cggtgccggg gctgacgcgc gcgcctaagt
     1561 ccgtgttcct cggcttgcga tacgctgacc gcgcgcaatg gatggcggcg ctgatccgcg
     1621 cggatgtccc cgtggatatt tacggccccg gttggggcaa tgatccagcg cccgagccac
     1681 cggcgacgag cgggtcggcc gccggtaaac ccgttcagcc gccgatgagc aaggcgtatg
     1741 cgcgcgaagt acagcggaac ttcacccaac atggccccat cggcggactg aagcgcacgc
     1801 tcgtgcaatg gaagatgcgc aaccagcagc gcgagatgat gccgctgttc aaaccgttcg
     1861 ccaaaggcgc ggtgccgttc acgcgcatcg gggagatctt ttccagctac gaagtcgtat
     1921 tgaatttcat caacgtctgg tcggacgggc ggacgggatc tgagctgatc ccgcacgtcc
     1981 gcctgcgcga ctttgaagcg ccaatgtcgc gcacctgctt catcacgggt tatacggacg
     2041 aaatcaccga gttttacgag atcggcacag aggtggacgc gtataagtcc gaagcggaat
     2101 tggtcgataa aaccaagtat tatctctcgc atccggacgc ggcggagcgc ctgcggcaag
     2161 ccgggtacga acgggcgctg cgcgaccaca cctgggtccg gcgattcgaa cagctctttt
     2221 ccatgatcgg aatgtcatga gcactgtcag cgcgggcggc aaggttctgc tggtcaccgg
     2281 atcgtccgga ttgatcggat cggaagtcgt cgaatatttc gccagccgcg ggtggacggt
     2341 gcacggcgtg gacaacaacc agcgcgccga tttcttcggc cccgcgggtg atacgcgctg
     2401 gaaccagcgg cgcttgctgg agaactatcg ctcgtcgttc caccatcacg aattagacat
     2461 ccgaaatcgc gacgcggtgc tgaagtgcgt cgccgaagtc aagccgaacc tgctcgtgca
     2521 cacggccgcg caaccgagcc atgacctggc ggcgtcccgg ccgttcgatg atttcgacgt
     2581 caacgcagtc ggcacgctga acatgctgga agcgacgcgc cggcacgcgc cggaaaccgt
     2641 cttcgtgcac atgagcacga acaaggtgta cggcgatcgg ccgaattcga tccggctgaa
     2701 ggagctggac aagcggtggg attacgatga ccccgcgttc gccgacggca tcgcggaaga
     2761 tttttcgatc gaccagagca agcacagcat cttcggcgcg tcgaaggtgg caggcgatgt
     2821 gatggtgcag gagtacggcc gatatttcgg catccgatcg tgcgttctcc gcggcgggtg
     2881 tttgaccggc ccgaagcaca gtggcgtcga gctgcacggc tttttgagct acctgatcaa
     2941 gtgcaacctg gaagggaagc tgtatcgcgt gttcggctat aagggcaagc aggtgcgcga
     3001 caacatccac tcgctggacg tcgcccgctt catcgccgcc ttttatgaaa acccgcgcat
     3061 cgcagaggtc tacaacctgg gcggcgggcg cgcgaacagc tgctcgatct gggaagcgtt
     3121 cgatcgcatt gcatcgctaa gtggcaagaa gatggtgcac gaatacgtgg acaagaaccg
     3181 cgaaggcgac cacatctgct acatcagcaa cctgtcaaag atgcggcggc attacccgtc
     3241 gtgggacatc agcaagtcgc tggacgacat cttccgcgag gtgtaccaga gctggcgcgc
     3301 ggggcatcag aagtaaacga gtctccttaa tagccggggg cggaagcccc ggtcttttcg
     3361 cgtttgaacc gaccggggct tccgcccccg gctatcacga gcgaatcatt tggcggcgca
     3421 acccgtagaa cccaaacccc cgcacatgcc gcagctggac ggcttgcgcg cgttcgccgt
     3481 gataggcgtt tggatcaccc actggaggat cggggaactc cccggttggc gccgcgtgcc
     3541 ctggggcaac ctcggcgttc
//
LOCUS       DATIMH010000831         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_778680, whole genome shotgun sequence.
ACCESSION   DATIMH010000831 DATIMH010000000
VERSION     DATIMH010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778680"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1964)
                     /locus_tag="VLI90_14410"
     CDS             complement(<1..1964)
                     /locus_tag="VLI90_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV15449.1"
                     /translation="MSTQGTSAAPPSPAIRSASHAPAGRPVSGDGAPAVSTTHPISSG
                     IAGSDQLDNRALLHWMFRFIRPVKGLAVVACIYTAMGVGADVLTTRQIGQAVDHIKNL
                     HVLAGSLQVPSSFSAWIGSADPQAVTLRQLVGVLFALVAMISLLRYLREVANSKFSMN
                     MVFYIREAVYDKVQHVGFGFHDALSSGQLINRALTDLQNVRSFVQTALLTTLEIVLVV
                     SGYIVLLTTRNPYIAVLSIVPLPLWVWYILRFSRRVQPAAKAAMEAGDRNVALITENI
                     AGVHVIKAFATERQEIEKYNGNCDEFLNRTLHRIRLFANFNPIIRLIASTTNLSLTFA
                     VAVLVLLRRLQVGDFLVLQAAMSAILGRLQGVAQINEQYQNAIVSARRLYEVLMADST
                     VPQRPHAKPLLAGPGNVRFEHVTFGYDASKPVLQDIDIEIKGGSIVAVVGPTGAGKST
                     LVNLIARFYDPQHGRVVLDGTDLRDVRLDSLRREVAFVFQETYLFSDTIEANIAYGRP
                     HITGGEVEAAARLAQAHEFIEKMPLQYQTILRERGSSLSGGQRQRLAIARALLTNPRV
                     LVLDDATAAVDPETEDLIRKGMKFAMYGRTTFVIAHRLSTVRAADMVIVLEQGRITQV
                     GRHSELMSQSGHYRDIAVAQLYSDHELPGD"
     gene            complement(2206..3453)
                     /locus_tag="VLI90_14415"
     CDS             complement(2206..3453)
                     /locus_tag="VLI90_14415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HSV15450.1"
                     /translation="MADSSPTQANEKPQEPPPLRSVHTNTFAQILHVLGASLAVTTYQ
                     AGKLVLLRPEPRADGPVLNTHFRAFHRPMGFAWERGRFALGTTTEIWEFHDLPAVASK
                     LDTPESPARHDAAFLPRTTHHTGDVQVHEMVWVPPDAGSETLRRPGSLAHEKPGSSPI
                     RSDIGVGLSELWFVNTRFSCLATRSDIYSFVPRWMPPFVTSLAPEDRCHLNGLALRDG
                     QPRYVTALGETDKPGGWRENKPAGGCVIDVRSNQTIARGLSMPHSPRWHNGQLWVLES
                     GNGGVGVIDQANGKYREICRLPGFTRGFDFAGPYAFIGLSQVRESAVFSGITIAEMKQ
                     EDRCCGVWAIDTRTGQTVGFVKFLDAVQEVFAVQVLNGIRWPEVLSEDTERLAQSYEL
                     PPAALRMVPEQLVQRTGQVEQTK"
     gene            complement(3490..>3989)
                     /locus_tag="VLI90_14420"
     CDS             complement(3490..>3989)
                     /locus_tag="VLI90_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HSV15451.1"
                     /translation="GNPGVTAPGLSFKYGYIEARLKAAPGWGLWSALWMLPTPDANGT
                     FHDGAGELDIAEIIGRSPGTVTMFTHKSSISHGRDYDTKINLTQAFHTYGLDWEPDHL
                     TWFIDNKQVFTVTDVASIPQVAEYLIMNLAVGTAGSWEDAPNSSTVFPTTMQVDWIHV
                     WQKNS"
BASE COUNT          672 a         1245 c         1325 g          747 t
ORIGIN      
        1 tcgtcgccgg gtaattcgtg atcgctatac aactgcgcga ccgcgatgtc gcgataatgg
       61 ccggactggc tcatcaactc gctgtgccgg ccgacctgcg tgatccggcc ctgctccagc
      121 acgatcacca tgtcggcggc acgcacggtg ctcaggcggt gggcgatgac gaacgtcgtt
      181 cgcccgtaca tcgcgaactt cattcccttg cggatcaggt cttcggtctc cggatcgacc
      241 gccgccgtcg cgtcgtccag caccagcacg cgcgggttgg tcagcagggc tcgcgcgatg
      301 gccaggcgct ggcgttgccc gccggacagg ctgctgccgc gctcgcgcag gatcgtttga
      361 tattgcagcg gcatcttctc gatgaactcg tgcgcctgcg cgagccgggc cgcagcttcc
      421 acttcaccgc cggtgatgtg cgggcgaccg taggcgatgt tcgcttcgat cgtgtcgctg
      481 aacaggtacg tttcctggaa gacgaaggcc acctcgcggc gaaggctatc cagccgtacg
      541 tcgcgcaggt cggtgccgtc caaaaccacg cggccatgct gcgggtcgta gaagcgcgcg
      601 atcagattga ccagcgtgct cttgccggcg ccggtcgggc cgacgaccgc gacgatgctg
      661 ccgcctttga tctcgatgtc gatgtcctgc agcaccggct tgctcgcgtc gtagccgaac
      721 gtcacgtgct cgaaccgcac gttgcccggg cctgccagca gcggcttggc gtgcgggcgc
      781 tgcggcaccg tcgagtcggc catcagcacc tcgtacaacc ggcgggccga gacgatcgca
      841 ttttgatatt gctcgttgat ctgcgcgacg ccctgcaggc ggccgaggat cgcgctcatc
      901 gcggcctgca gcacgaggaa gtcgcccacc tgcaagcggc gcaggagcac cagcaccgcc
      961 acggcgaagg tgagcgacag gttcgttgtg ctggcgatca ggcggataat cgggttgaag
     1021 ttggcgaaca gccgaatgcg gtgcagcgtg cgattgagaa attcatcgca gttgccgttg
     1081 tatttttcga tctcctgccg ctcggtggcg aacgccttga tcacgtgcac gccggcgatg
     1141 ttttcggtga tgagggcgac gttgcggtcg ccggcttcca ttgccgcctt ggccgccggt
     1201 tgcacgcgcc ggctgaagcg caggatgtac cacacccaca acggcagcgg cacgatcgac
     1261 agcaccgcga tgtacgggtt gcgcgtcgtc agcagcacga tatagccgct gacgaccagc
     1321 acgatctcca gcgtcgtcag cagcgcggtc tgaacgaagc ttcgcacgtt ctgcaaatcg
     1381 gtcagcgcgc ggttgatcag ttggccgctc gagagcgcat cgtggaagcc gaagccgacg
     1441 tgctggactt tgtcgtacac ggcctctcgg atgtagaaga ccatgttcat tgagaacttg
     1501 ctgttggcca cctcgcggag gtagcgcagc agcgagatca tcgcgaccag ggcaaacagc
     1561 acgccaacca gctggcgaag cgtgacggcc tgcggatcgg cgctgccgat ccacgcgctg
     1621 aagctgctcg gcacctggag cgagccggcg aggacgtgca aatttttgat gtgatcgacg
     1681 gcttggccaa tctgccgggt ggtcagcacg tccgccccga cgcccatcgc ggtgtagatg
     1741 catgccacca ccgcaaggcc tttgaccggg cggatgaatc ggaacatcca gtggagcagg
     1801 gcgcggttat cgagctggtc gctgccggcg atgccgctcg agatcggatg cgtggtggaa
     1861 acggcgggcg cgccgtctcc gctcaccggc cggccggcag gcgcgtgcga tgcagagcga
     1921 atggccggcg acggtggcgc ggcggaggtg ccttgggtac tcacggttga ctgcagtgtg
     1981 tcggaaacga tgcaagctac tgtctgcccc ccgaacgtca acccttggca gggatgaatt
     2041 tgcaccatcc tttgcagaaa tggcgattcg gcgggctttg gggtgcgtaa agagcaggaa
     2101 gtggagccca cgccgggttc ggagcgtaat cgcgacgacc cggattcttc tggcgcgaga
     2161 gatgcgggtc gtcggagtac ctccgaaccc ggcgtggact ggcgcctatt tcgtctgctc
     2221 gacttgaccc gtccgctgca ccagctgctc cgggaccatg cgcagcgccg ccggtggcaa
     2281 ttcatagctt tgggccagcc gctcggtgtc ttcgctaagg acttccggcc agcggatacc
     2341 gttcaagacc tgcacggcga acacttcctg aaccgcgtcg aggaacttca cgaagccgac
     2401 ggtttggccg gtgcgcgtat caatcgccca cacgccgcag cagcggtctt cttgtttcat
     2461 ctcggcgatg gtgatgccgc tgaagacggc ggattcgcgc acctgggaca ggccgatgaa
     2521 cgcgtacgga ccagcgaaat cgaacccgcg ggtgaagccg ggcagccggc agatctcgcg
     2581 atatttgccg ttggcttgat cgatcacacc gacgccgccg ttgccgcttt ccaggaccca
     2641 cagttgtccg ttgtgccagc gcggcgagtg cggcatcgac aggccgcggg cgatcgtctg
     2701 gttgcttctg acgtcgatca cgcagccgcc ggcgggtttg ttttcgcgcc acccgccggg
     2761 cttgtcggtt tcacccagcg cggtgacata gcgcggctgg ccatcgcgca gcgcgaggcc
     2821 gttcagatga cagcggtctt ccggcgccag tgaggtaacg aacggcggca tccaccgcgg
     2881 gacgaaactg tagatatcgc tgcgggtcgc caggcaggag aagcgcgtgt tgacgaacca
     2941 gagttcggac agcccgacac cgatgtctga gcgtattggc gaagacccgg gcttttcgtg
     3001 cgcaagtgat ccgggtcttc ggagtgtctc agacccggcg tcgggcggca cccacaccat
     3061 ttcatgcacc tgcacgtcac cggtgtgatg cgttgtgcgc ggcaggaacg ccgcgtcgtg
     3121 tcgcgccggt gattccggcg tgtccagctt cgacgccacc gcgggcaggt cgtggaactc
     3181 ccagatttcc gtcgtcgttc ccagcgcgaa gcgcccgcgc tcccaggcga aacccatggg
     3241 ccgatggaac gcgcggaagt gcgtgttcaa taccgggcca tccgcgcgcg gctccggtcg
     3301 cagcagcacc agctttcccg cctgataggt tgtaacggcc agcgatgcgc ccaggacgtg
     3361 caggatctgc gcgaacgtgt tcgtgtgcac gctgcgcagc ggcgggggct cctgcggttt
     3421 ttcgttcgct tgagtcggtg atgaatcggc catcggtcat cgccggcgat gggcggggga
     3481 gtcaatcgct cacgaattct tctgccacac gtggatccaa tcgacctgca tggtcgtcgg
     3541 aaagacggtg ctgctgttgg gcgcgtcttc ccacgaaccg gcggtgccga ccgcgaggtt
     3601 catgatcagg tattccgcca cctgcgggat tgacgctacg tccgtcacgg tgaacacctg
     3661 cttgttgtcg atgaaccacg tcaggtgatc cggctcccaa tccaaaccgt acgtgtggaa
     3721 cgcctgcgtg aggttgatct tggtgtcgta atctctgccg tggctgatgc tgctcttatg
     3781 cgtgaacatc gtaacggtgc cgggcgagcg gccaatgatc tcggcgatgt ccagctcacc
     3841 ggcgccgtcg tggaacgtgc cgttggcatc gggcgtcggc agcatccaca gggcgctcca
     3901 caggccccag cccggggcgg ctttgaggcg cgcttcgatg tagccgtact tgaaggacaa
     3961 gcctggcgct gtaacgccgg ggttgccac
//
LOCUS       DATIMH010000832         1961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4240553, whole genome shotgun sequence.
ACCESSION   DATIMH010000832 DATIMH010000000
VERSION     DATIMH010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4240553"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VLI90_14425"
     CDS             complement(<1..696)
                     /locus_tag="VLI90_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15452.1"
                     /translation="MADWRATGLDSSREWRTILPIAIVVIVFRSAIFVFWPQSYFDSD
                     QAIFGLMAKHIAEGRAFPLFMYGQTYMLAAEAWLAAPLFLVAGASVAALKFPLLIINI
                     AIALLLARMFYREVGLRPWLALVATLFFVLPAPGTSAMFVEAGGGNIETPLYVILIWL
                     TRNRPNWCGLVLGIGFVNREFTLYGLAALLTIEAAHRVLFTRQGILRRLRMFRTAAEV
                     WLAVTWLKQYSPAA"
     gene            724..1503
                     /locus_tag="VLI90_14430"
     CDS             724..1503
                     /locus_tag="VLI90_14430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSV15453.1"
                     /translation="MPDIPRAQDQLFDRRSSAREKYAALVVGRADWPSLIAHECVTLA
                     SQSIPGALGLVLRRALYPLLLGACGRNVVFGQNVVLRHPHKIRIGDDVVVDDNCLIDA
                     KGDSNRGITIGNRVFIGRNTILSCKNGDIDVDDGANVGFNCEIFSASSVRVGADTLIA
                     AYCYLIGGEHDFSDPSQPVLAQARRSTGVTIGAGAWLGAGAKILDGVSVGERAVIGAG
                     AVVREAVPSGSVAVGIPARVIGQRASVGDEPAAAAPGLEHR"
     gene            1500..>1961
                     /locus_tag="VLI90_14435"
     CDS             1500..>1961
                     /locus_tag="VLI90_14435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV15454.1"
                     /translation="MKLNVLQVCDHLGWEGSRMHGVKRLFAWMIPRFDAERYNVSLVS
                     LRKKDLSEETLESFGVDITYLHKSKFDPATLPALLKVIDRKKIDILHLHGYGATTFGR
                     IAGAMRRLPTIVHEHANLTDTPWFQKIADRALEPVTDIAIAVSQSTADFVIR"
BASE COUNT          382 a          584 c          649 g          346 t
ORIGIN      
        1 ggccgccggt gagtactgct tcagccaggt cacggccagc cacacttccg ccgccgtcct
       61 gaacattcgt aagcgtcgaa ggatgccttg ccgcgtgaag agcactcgat gcgccgcttc
      121 gatcgtcagc agcgcggcca gaccgtacag tgtgaactcg cggttgacga aaccgattcc
      181 cagcaccagc ccgcaccaat tcggacggtt gcgcgtcaac cagatcaaga tcacataaag
      241 aggagtttcg atgttcccgc cgccggcttc gacaaacatg gcggaggtgc caggcgcggg
      301 cagcacgaaa aacaacgtcg cgaccagggc gagccacggg cggaggccga cttcgcgata
      361 aaacatccgc gcgagcagca gggcaatcgc gatgttgatg atgaggaggg gaaacttcag
      421 cgcggcgacc gatgcgccgg cgaccaggaa gaggggtgcg gcgagccacg cttcggcggc
      481 gagcatgtag gtctggccat acatgaaaag cgggaaggca cgtccttcgg cgatgtgctt
      541 tgccatcagg ccgaagatcg cttgatccga atcgaagtag gactgcggcc agaagacgaa
      601 gatcgcggat cggaaaacga tcacgacgat cgcgatggga aggatggtcc gccattcgcg
      661 cgagctgtcg aggcctgtcg cgcgccagtc ggccatgcgg gccggtatta tatgctatcg
      721 gcgatgcccg acattccgcg cgcgcaggat cagcttttcg atcggcgctc gtcggcgcgc
      781 gaaaaatatg cggcgctggt ggtggggcgt gcggactggc cgtcactcat cgcacatgaa
      841 tgcgtcacgc tcgcgtcgca gagcattccc ggcgcgctcg gtctcgtact tcgccgcgcc
      901 ctttatccat tactgctcgg ggcctgcggc cgaaacgtcg tgttcggaca gaacgtcgtg
      961 ctgcgccatc ctcacaagat ccgcattggc gacgatgtcg tggttgacga caattgcctg
     1021 atcgacgcca agggggactc gaatcgcggt attacgattg gtaaccgcgt gttcatcggt
     1081 cgcaacacga ttctgtcgtg caagaacggc gacatcgacg tggacgacgg cgccaatgtc
     1141 ggattcaact gcgagatctt ttcagccagt tcggtgcggg ttggcgcaga cacgttaatc
     1201 gccgcgtact gctatctcat aggcggcgag catgatttca gcgacccgtc gcaaccggtg
     1261 ctcgcgcaag cgcggcggtc gaccggtgtc accatcggcg cgggtgcgtg gctcggcgcg
     1321 ggcgcgaaga tcctcgacgg cgtgtcggtc ggcgagcggg cggtgattgg ggcgggcgcg
     1381 gtcgtgcgtg aagcggtgcc gagcggatcc gtcgcggtcg gcattccggc acgggtgatc
     1441 gggcagcgcg cctccgtcgg cgacgaaccc gccgccgcgg ctccagggtt ggagcaccgg
     1501 tgaagctcaa cgtcctgcag gtttgcgatc atctcggctg ggaaggatcg cgcatgcacg
     1561 gcgtcaagcg gctgtttgcg tggatgatcc cgcggttcga tgccgagcgc tacaacgtct
     1621 cgctggtgag tctgcgcaag aaggatctct ccgaggagac gctggaatca tttggcgtcg
     1681 acatcaccta tcttcacaaa tccaagttcg atccggcgac gctgccagcg ctgctgaaag
     1741 tcatcgatcg aaagaagatc gatatcctgc acctgcacgg gtatggcgcg acgacgttcg
     1801 gcaggatcgc cggtgcgatg cggcggctgc cgaccatcgt gcacgagcac gccaacctga
     1861 ccgacacgcc ctggttccag aaaatcgccg atcgtgcgct cgagccagtc accgatatcg
     1921 ccatcgcggt gtcgcagagc acggcggact ttgtgatcag a
//
LOCUS       DATIMH010000833         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2218502, whole genome shotgun sequence.
ACCESSION   DATIMH010000833 DATIMH010000000
VERSION     DATIMH010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218502"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="VLI90_14440"
     CDS             <1..1289
                     /locus_tag="VLI90_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HSV15455.1"
                     /translation="PVAANTLLTSKDFEFMLRDSRAQAFFVSKPLLPVIEPLIGNVRT
                     LKSLVIAGDSGANSVAGWIESGTATAETASTCADDACFWLYSSGSTGTPKGTVHLHSH
                     LIQTAELYGRAVLGIRESDVVYSAAKLFFAYGLGNGLTFPMSVGATALLLPARPAPPD
                     VFAVLRNYRPTIFYGVPTLYAGLLADPAKPSREELNLRVCTSAGEALPADIGKRWTAH
                     FGCEILDGIGSTEMLHIFLSNRPGEVRYGTTGKAVPGYELRIIGDDGRECGDGEIGEL
                     QISGPSAALMYWNNRAKTKSTFAGEWTRSGDKYTRDSDGYYTYGGRSDDMLKVGGIYV
                     SPFEVEACLMTHAAVLEVAVIGAADIDELVKPKAYVVLKPGQGVSREALQAHVKSLLA
                     PYKYPRWIEFVPELPKTATGKIQRFKLRTSNPSATA"
     gene            1489..>2418
                     /locus_tag="VLI90_14445"
     CDS             1489..>2418
                     /locus_tag="VLI90_14445"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSV15456.1"
                     /translation="MGFMLPYTGTYAQLGVAIENGMRMAIDENGGKLGGREIEWFKVD
                     DESEPSKGVENATKLVQRDKVDVIVGTVHSGVQMGIQKVARESGVLSLIPNAGVHAAT
                     RALCAPNVFRTSFTNSQPTLALGKVMVDKGHKKAVWITWKYAAGDDAFEGFKESYTKA
                     GGTIIKELGLPFPNVEFQALLTEIASLKPDAVACFFAGGGAAKFIRDYAAAGLKGRIP
                     LYGSGFLTEGVLDAAGPAAEGILTTLHYSDSLDTPRNKQFRLAYAKKFHLQPDVYAVQ
                     GYDTGLLLSQGAAAVKGDLNNKQALYKALESAVI"
BASE COUNT          464 a          767 c          758 g          429 t
ORIGIN      
        1 tgccggtggc cgcgaacacg ctgctgacca gcaaggattt cgaattcatg ctgcgcgact
       61 cgcgagcgca agcttttttc gtttccaagc cgctgcttcc cgtcatcgag ccgttgatcg
      121 gcaacgtgcg cacgctcaag agcctcgtga tcgccggcga cagcggcgcc aattccgtgg
      181 ccggctggat cgagtccggc accgcgacgg cggagactgc gtcgacctgc gccgacgatg
      241 cgtgcttctg gctttattcg agcggctcca ccggcacacc caagggcacg gtacatctgc
      301 acagccatct gatccagaca gcggagttgt acggccgcgc cgttctgggg atccgtgaaa
      361 gcgacgtggt ctactccgcg gccaagctgt tctttgcgta cggcttgggc aacggcctga
      421 cgtttcccat gagcgtcggc gccaccgcgc tgttgctgcc cgcgcgaccg gcaccgcctg
      481 atgtcttcgc tgtcctgcgc aattaccggc cgacgatttt ttacggagta cccacgctgt
      541 atgcggggct gctggccgac cctgcgaaac cctctcgcga agaactcaat ctgcgcgtgt
      601 gcaccagtgc gggtgaggca ctcccggccg acatcggcaa gcgctggacc gctcacttcg
      661 gatgcgagat tctcgatggg atcggatcca ccgagatgct gcacatcttc ctgtcgaatc
      721 gccctggcga agtgcgttat ggcaccaccg gcaaagcggt tcctgggtat gaattgagga
      781 tcatcggcga cgacggccgc gagtgcggcg acggcgagat cggtgaactg caaatcagcg
      841 gtccgagtgc ggccctcatg tactggaaca accgcgcgaa gactaagtcg acgttcgccg
      901 gcgagtggac ccgcagcggc gacaagtaca cccgcgattc ggatggctac tacacgtacg
      961 gcgggcgcag cgacgacatg ctcaaggtcg gcggtatcta cgtctcgccc ttcgaggtcg
     1021 aggcctgcct catgacacac gccgcggtgc tcgaagtcgc cgtgatcggg gcggccgata
     1081 tcgatgaact cgtgaagccg aaggcctacg tcgtgctcaa gcccggccag ggcgtgagtc
     1141 gcgaagcctt gcaggcacac gtcaagagct tgctggcgcc gtacaagtac ccgcgctgga
     1201 tcgagttcgt ccccgaattg cccaagaccg ctaccggcaa gatccagcgt ttcaagctca
     1261 ggacttccaa tccatcagcg actgcgtgaa cacctcgcag cgcgaattcg ggtaagccat
     1321 gtgtgccgga tgagttcatc cggagacaat accgccagct ttgatcaagg agacttttga
     1381 atgaccaccc gccgtctcgt cctcacccgc agcgccgccg tcgtgggcgc cgcatccacc
     1441 gggctgttgc tcccgcaaat cgttcgtgca cagtcgaaca aggttcgcgt gggtttcatg
     1501 ctgccgtaca ccggcacgta cgcgcagctt ggcgtcgcga tcgagaacgg catgcgcatg
     1561 gccatcgacg aaaacggcgg caagctgggt ggccgcgaaa tcgaatggtt caaggtcgac
     1621 gatgagtccg agccttcgaa aggcgttgaa aacgccacga agctggtcca gcgcgacaag
     1681 gtcgacgtga tcgtcggcac ggtgcactcg ggcgtgcaaa tggggatcca gaaggtcgca
     1741 cgcgagagcg gcgtgctgag cctgattccg aacgcgggtg tccacgcggc tactcgcgcg
     1801 ctgtgcgccc ccaacgtgtt ccgcacatcg ttcaccaact cgcagccgac gctggcgctt
     1861 ggcaaggtca tggtcgacaa gggccacaag aaggctgtct ggatcacgtg gaaatacgcg
     1921 gccggcgacg acgccttcga aggtttcaag gaaagctaca cgaaggcggg cggcaccatc
     1981 atcaaggaac tgggactgcc cttccccaac gtggagtttc aagcgctcct caccgagatc
     2041 gcgtcgctca agcccgatgc ggtcgcatgc ttcttcgccg gcggcggcgc ggccaaattc
     2101 attcgggact acgcggccgc gggcctcaaa ggccgtatcc cgctgtatgg gtcgggcttc
     2161 ctgaccgaag gcgtgctcga tgcggccggc cccgcggccg agggcatcct gaccacgctg
     2221 cattacagcg attcactgga cacgccgcgg aacaagcagt tccgcctcgc ctacgcgaaa
     2281 aagttccacc tgcagcccga tgtgtatgcg gttcagggct acgacaccgg actgctgctg
     2341 tcgcagggcg cggcagctgt gaagggtgac ctgaacaaca agcaggcgct gtacaaggcc
     2401 ctggagtccg cggtgatc
//
LOCUS       DATIMH010000834         5305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4241261, whole genome shotgun sequence.
ACCESSION   DATIMH010000834 DATIMH010000000
VERSION     DATIMH010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4241261"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1029
                     /locus_tag="VLI90_14450"
     CDS             190..1029
                     /locus_tag="VLI90_14450"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSV15457.1"
                     /translation="MMHQSSVGASRGAAVAVAFLCIAGTASADDTGAGGNACKDLPGY
                     AALKSALAAATATETSGLNNQMWGTIVDRDGIVCAIAFTGVNRGAQWPGSRVISAQKA
                     NTANAFSLDASSNGGGSGQPSGLALSTANLYSAVQPGGSLFGLQFSNPVNADVAYEGP
                     SAHYGTANDPMVGSKIGGVNVFGGGLALYAVGKKIIGAVGVSGDTSCADHNIAWRVRN
                     NLGLDHLSGVGGVSGDAARPDNIIFDIVPNPNGGTGVSAGGFGHPTCPNTSGSGTLPP
                     VQP"
     gene            complement(1180..1710)
                     /locus_tag="VLI90_14455"
     CDS             complement(1180..1710)
                     /locus_tag="VLI90_14455"
                     /inference="COORDINATES: protein motif:HMM:NF023113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HSV15458.1"
                     /translation="MLGVAMAQETPWRDTPPSSVSRSQADGVQLKTIAILDFDLVDDQ
                     HELSPATVEYQRLGAIRDQLQDEFAKNGLYRIVDPAPAAELIKMHQSKSQLHECNGCE
                     LNIARALHADRVLIGWVQKVSNLILNINIQIEDVATGAVLLNKSVDLRGNTDETWRRG
                     ISFLVKSMVEKSQGNR"
     gene            1795..2340
                     /locus_tag="VLI90_14460"
     CDS             1795..2340
                     /locus_tag="VLI90_14460"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HSV15459.1"
                     /translation="MKAVAQSSAGAPLLQMGVVNNLDSIGVTAPVVDVVIEVPRGSFV
                     KRGSNAHVDFVSPIPCPYNYGSVPTHIGLEGDLLDALVLGPRLAFGATVRVTAWGAVT
                     LMDRGMVDDKLICGGTRLTSAERKAVLRFFRFYAMCKGMLNAWRGQSGRNACDGWCTA
                     EFALARAKLRDETWPGSAVEF"
     gene            2485..3747
                     /locus_tag="VLI90_14465"
     CDS             2485..3747
                     /locus_tag="VLI90_14465"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV15460.1"
                     /translation="MHHPGYRRYIATYVLAMMADNIEHVISYWKVFQNFHSPALGGFA
                     VLSHWLPFLFFSVPVGALGDRFDPRRIIQFGMLLFVIASLSWGILFLIDALTMWEAML
                     ILVIHGCAGVFWQTSSQLLLHDIVPVEQLQSAVRLNATGRYLGVLVGPAVGGAILLAL
                     GPAHGILLNALFYVPVILWLWKAPYGPRFRHGKPPPQRPVRGFADIVQTFRDVAGHRA
                     IAAMILLAGAASFLVGNSYQAQMPGFAQDLGHGDPGVSYSMLLAADAAGALTAGILLE
                     SVAVLRPRIATALLFAGAWCMALAAFALTSAYPLALALLFAAGFFELSFSSIAQTVVQ
                     MDAPAGIRGRVIGLYNMSSLGLRAFSGVTVGLIGAIVGIHHSLAGASIVLLVLIAALL
                     ILRTPSPRTGNMPAGEREAASPGRSRET"
     gene            3926..4663
                     /locus_tag="VLI90_14470"
     CDS             3926..4663
                     /locus_tag="VLI90_14470"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV15461.1"
                     /translation="MNSLTVEDLASLDQFHTRGLAATRELIDFAGVRQGWPVLDVGSG
                     LGGPARVIASKMKCHVTGVDITKEFCDAATMLSKLTSLENVTDFRHADATALPFDDDQ
                     FDLVLTIQVQMSIEPKERFYSEIFRVLKPGGRFAFQDIMSGPGGDIVLPVPWATRREL
                     NFLIAVDELHEILNKQGFHTEKFEDISNEALVWRRQQPVSAGLSASPLGLQVVMGDQF
                     AEMQSNQIRNLEQRRVVFVRGLTTKPM"
     gene            4898..>5305
                     /locus_tag="VLI90_14475"
     CDS             4898..>5305
                     /locus_tag="VLI90_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15462.1"
                     /translation="MAALLGLATPIMSSAQGAGAVVRGPVSGDQDELPQSGSNRPFLD
                     SVNIELGYSYDDNVTLGRTGDEILRDQSFALGASASRAFRIGDKTRLVVTGLLNGEKF
                     NRYNGLSNLSGGLEAEIQYRQSGAFDAITYAAFA"
BASE COUNT          964 a         1602 c         1669 g         1070 t
ORIGIN      
        1 ggccgtccat ggcgcgactg tacaccgtag cctacaaatc gatgatgtcg acgaatcacc
       61 gcccgggtca ttcccgatca gcggtttgtt gcactcgaag ctgaaggctc ctagagtcgc
      121 gacgagtgcc aatcgcgtgg caaccaacgg accacagcac tcacattcga actgaccgag
      181 gagcaagaaa tgatgcacca atcatctgta ggggcgtcaa gaggggcggc ggttgcggtc
      241 gcgtttttat gcatcgccgg cacagcgagt gccgacgata ccggggcagg gggcaacgca
      301 tgcaaagatc tgcccggcta tgcggcgctc aagtcggcgc tggccgccgc aacggcaacg
      361 gagaccagcg gtctcaacaa ccagatgtgg ggaacgatcg tggaccggga cgggattgtg
      421 tgcgcgatcg ccttcaccgg cgtcaatcgc ggcgcgcagt ggcccggcag ccgggttatc
      481 tcggctcaga aggcgaacac cgcgaatgcg ttcagtctcg atgcgtcgtc gaacggcggc
      541 ggatcgggac aacccagtgg cctggccctg tcgacggcca acctgtactc cgcggtgcag
      601 cccggtggca gcctgttcgg attgcagttc agcaacccgg taaatgccga tgtggcgtac
      661 gaggggcctt ctgcgcatta cgggaccgcg aacgatccga tggtcggaag caagatcggt
      721 ggcgtcaacg tgttcggggg cgggcttgcc ctctacgcgg tgggcaagaa gatcatcggt
      781 gcggtcgggg tgagcggaga cacatcgtgc gcagaccaca acatcgcctg gcgcgtccgg
      841 aataatttgg gtctcgacca tctgtcgggt gtcggcggag tgtcgggtga tgccgcccgt
      901 cctgacaaca tcatcttcga catcgtgccc aatccgaatg gcggaaccgg ggttagcgct
      961 ggcgggttcg gtcacccaac ctgtcccaac acctcgggat cgggtacgtt gccgcccgta
     1021 cagccataac tcgatgtgca actcggcacg ctgattccgg cgggtagccg gaggatgcct
     1081 tccggtggcg cggaggcgac gaaaaccgcg cgctccgcgc caccagtgcc cagggggatg
     1141 taccaagtat ttctgtgcac gacgacgccc tcattgcagc taacgattac cctgactttt
     1201 ttcgaccatg cttttgacca agaacgatat gccgcgtcgc caggtctcat cggtgttacc
     1261 acgcagatca accgatttgt tcagcaatac agcgcccgtt gcgacatctt cgatctgaat
     1321 gttgatattg aggatcaggt tgctgacctt ctggacccag ccaatcagga ctcgatcggc
     1381 gtgcagcgct cgagctatgt ttagttcgca tccgttgcat tcgtgcagct gggatttgga
     1441 ctgatgcatc ttgatcagct cggcagccgg tgccgggtcg acgatgcgat acagcccgtt
     1501 cttggcgaac tcgtcttgca actggtcccg gatcgcacca agccgctggt attcgacggt
     1561 tgcgggtgac agctcgtgtt gatcgtccac gaggtcgaaa tcaaggatcg caatcgtctt
     1621 gagctgtacg ccatccgctt gcgaccggga aaccgatgat gggggagtgt cacgccacgg
     1681 cgtctcttgc gccatggcaa cgccgagcac caagaacaga cataccggag ttacagcaaa
     1741 gcgcatcacc cggcccccgt ggccgaagca ttggacaaca cgtaagcttc tgccgtgaaa
     1801 gcggtcgcac agagcagcgc aggcgctcct ctgctgcaaa tgggggtagt gaacaatctg
     1861 gactccatcg gcgtaaccgc tccggttgtg gatgttgtga tcgaagtgcc gcgcggcagc
     1921 ttcgtgaaac ggggctcgaa cgcgcatgtc gatttcgttt ccccgatacc ttgcccctac
     1981 aactatgggt cggttcctac gcatatcggc ctggagggcg acctgcttga cgcgctcgtc
     2041 cttggtcccc gactcgcgtt tggagcgacg gtgagggtta cggcgtgggg cgcggtgacg
     2101 ctgatggatc gaggcatggt agacgataag ctgatctgcg gcgggacacg gttgaccagc
     2161 gccgagcgta aagccgtact tcgctttttc cgcttctacg cgatgtgcaa gggaatgctg
     2221 aatgcgtggc gcgggcaatc aggacgaaac gcgtgcgatg gatggtgcac ggcagagttt
     2281 gcgttggcgc gagccaagct gcgtgacgaa acgtggcccg gctccgctgt cgagttttga
     2341 gcgggcgtcg cccccggaca cctccttcga tcgcgccaca ccatgcggcg gccatgccgt
     2401 gtccgcatcg cgctactcgc ttccgtgagc aatccattga gccgtcgtgc tgcatcccgc
     2461 gcctcgccac gctcgttcgc ggcgatgcac caccccggct atcgtcgcta cattgcgacc
     2521 tacgtgctcg cgatgatggc cgacaacatc gagcatgtga tcagctactg gaaggtgttc
     2581 cagaattttc attcacccgc gctcggcggc ttcgccgtcc tctcgcattg gctgccgttc
     2641 ctgttcttct cggtgccggt aggcgcgctc ggcgaccgct tcgatccgcg ccgcatcatc
     2701 cagttcggga tgctgctgtt cgtgattgcg tcgctgtcgt ggggcattct gttcctcatc
     2761 gacgcgctca cgatgtggga agcgatgctg atcctggtga tccacggttg cgcgggcgtg
     2821 ttctggcaga cgtcgagcca gctgctgttg cacgacatcg tgccggtcga gcaactgcaa
     2881 agcgcggtgc gcctcaatgc gacggggcgc tatctgggag tgctggtcgg tccggcggtg
     2941 ggcggcgcga tcctgctcgc gctcggacct gcgcacggaa tcctgctcaa cgcgctgttc
     3001 tacgtgccgg tcatcctgtg gctttggaag gcaccgtatg gtccgcgctt ccggcacggc
     3061 aagccaccgc cgcagcgccc ggtgcgcggc ttcgccgaca tcgtgcagac cttccgcgat
     3121 gtggccgggc atcgcgcgat cgcggcgatg atcctgcttg caggcgccgc gtcgttcctg
     3181 gtcggcaaca gctaccaggc gcagatgccg ggtttcgcac aggacctggg acacggtgat
     3241 ccgggcgtgt cgtacagcat gctgctggca gccgatgccg ccggcgcgct gacggcgggc
     3301 atcctgctcg aaagtgtcgc ggtcctgcgc ccgcgcatcg ccacggcgct gctgttcgcc
     3361 ggggcgtggt gcatggccct tgccgcgttc gcgctcacgt ccgcatatcc gctggcgctc
     3421 gcattgctgt tcgccgccgg tttcttcgag ctgtcgttca gcagcatcgc gcagacggtc
     3481 gtgcaaatgg atgcgccggc cggaatccgg ggccgcgtca tcggtctcta caacatgtcg
     3541 tcgctcggat tgcgcgcctt cagcggcgtg accgtggggc tgatcggagc aatcgtcggc
     3601 atccaccact cgctcgcagg cgcgtccatc gtgctgctgg tgctgatcgc ggcgttgctg
     3661 atcttaagaa caccatcgcc acgcactggc aacatgcccg ccggcgagcg agaggccgca
     3721 tcgcccggtc gatcgcgtga aacctgaagt gcgtcagtac cgctagaatt cagttccgct
     3781 gttcatgttt gtatcggcac cgcgttaccc ggtaacctcc tcggagaccg tttccatggc
     3841 ctcgagcaat tccgtatccc ggcactatgg gagcacagga ctgtccgaac gaattctcca
     3901 tgcactgagg caaggcggaa agaacttgaa ctctctcacc gtcgaggacc tcgcatcact
     3961 cgatcagttc cacacacgtg ggttggccgc gacgcgggaa cttatcgact tcgccggcgt
     4021 gcggcagggc tggccggttc tggatgtcgg gagcggtctt ggcggcccgg cacgagtcat
     4081 tgcgtcgaag atgaaatgcc acgtcaccgg tgtagacatc accaaggagt tttgcgacgc
     4141 ggcgacaatg ctatccaaat tgacgtcact tgaaaacgtc accgattttc gccacgcaga
     4201 cgccactgca ctgccgtttg acgacgatca gttcgatctc gttttgacaa tccaagtgca
     4261 aatgagcata gagccgaagg aacgctttta ctcagaaatc tttcgcgtgt tgaaaccggg
     4321 cggacgattc gctttccagg acatcatgtc tggtccgggc ggcgacatcg ttcttcccgt
     4381 tccctgggca actcggcgcg agttgaattt ccttatcgcg gtagatgagc tgcatgagat
     4441 tttgaacaaa cagggatttc acaccgaaaa gttcgaggat atctcgaacg aggctctggt
     4501 gtggcggaga cagcagccag tatcagcagg actgtcggcg tcgcccttgg gcctgcaagt
     4561 ggtcatgggc gatcagtttg ccgagatgca atcgaatcag attcgcaatc tggagcagcg
     4621 gcgggtggtt ttcgtgcgtg gactaacgac taaacctatg taatgcgtgg gcgggcaacc
     4681 ccttacgctg ccgagagaag gatgacagtt tattcgggac ggattcccgc acttggccgc
     4741 gctgtcgtag ggtaatatgg gcagcgtccc tcctgtccca tgatcgctag gcgtgctcct
     4801 gaatttccgg gacatagtct ttcggcgctc cccgagcgac cggcagcatc gcccgcttcc
     4861 acgcaggcgg tctagcggcc ggcgcactat cgcgatcgtg gcagcgctgc tcggtctggc
     4921 gacaccgata atgtcgtcgg cgcaaggcgc aggagcggtg gttcggggtc ccgtgtccgg
     4981 cgaccaggac gaactccccc agtccggatc gaatcggccc ttcctcgata gcgtcaacat
     5041 cgaactgggc tacagctacg acgacaacgt caccctgggc cgtaccggcg acgagatcct
     5101 gcgggaccaa tcgttcgcgc tcggcgccag tgcaagccgc gcgttccgca tcggcgacaa
     5161 gacacggctg gtcgtgacgg gcctcttgaa cggcgagaag ttcaacaggt acaacgggct
     5221 ctcgaatctt tcgggcggcc tcgaagcgga gattcagtat cggcagtccg gtgctttcga
     5281 cgcgataacg tacgcggcct tcgcg
//
LOCUS       DATIMH010000835         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5688219, whole genome shotgun sequence.
ACCESSION   DATIMH010000835 DATIMH010000000
VERSION     DATIMH010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5688219"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VLI90_14480"
     CDS             complement(<1..855)
                     /locus_tag="VLI90_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15463.1"
                     /translation="MRLHSTLGCLAALIAVAVASIDVRAEPPDRRGTTEFDFPKPDRI
                     RYDNHSTIDVTVAVDAGQTLRTMSPRRLGGTNVAMWYFATVFASPQVREWMNDLHPGY
                     IRLPGGSWSNLVYWNGHGVRGLDGVVDPNRVGPDEYPAVDYSDYAPSFLADSKTLHPA
                     GNNWHGHVDVKALQDFVKALPGVEPLACPNAGTGRPIDAAEWVKWANQKMGYNVRVWE
                     IGNELGGAWEAGSELPFGKGTLTAEMYTKRYNDMASAMRQVDPSIKIGGGAFAEQMLR
                     DCGTNVDFV"
     gene            complement(1104..2282)
                     /locus_tag="VLI90_14485"
     CDS             complement(1104..2282)
                     /locus_tag="VLI90_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSV15464.1"
                     /translation="MSPPPLRSSVTHRVAIIHTDDTLEAWDFCRGVASYRPRSGSWGL
                     RSIPIPFHVTPGRQRRLSKTVVDGVIMMPGRWRTLRPLHEGGVKVIEVDGDADRAPYA
                     RIRLDNRYIGQLAGEHLLERGFTRAIFLGARAAWSRERAGGLQQAFTSAQRECHLIFA
                     SDWIQLTSEAWIARQLRRFARPMAVMGCNDLIAASVVRAVNAARWQIPAEVGVIGVDD
                     RITECIGVQPPLSSISIERFGIGREAARLLDQAFMGRLDARVDQRFRPQGVIERESTS
                     TYVHEDPDVAAAIAYIRREACNGATIDDVVRSIHLSRRTLERRFAQVVGRPPGDEIRR
                     VRLEAVKHTIERTHLPLTDIAEQCGFSCLSSLSHSFRLATGISPQAYRRKVRSRGVAP
                     "
     gene            2443..>2781
                     /locus_tag="VLI90_14490"
     CDS             2443..>2781
                     /locus_tag="VLI90_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HSV15465.1"
                     /translation="MTVSLGLLLMRLMTPAAWAGSSGAAASPASDNPAPPDAWVAVAQ
                     MTPGINIGNTLEATAGWETGWGNPPITKEYVQTLARLGFKTVRLPVAWDTYADNGRIT
                     QKQFDRVAEVV"
BASE COUNT          480 a          917 c          908 g          476 t
ORIGIN      
        1 gacgaaatcg acgttggtcc cgcagtcgcg gagcatttgt tcggcgaacg cgccgccgcc
       61 gatcttgatc gacgggtcca cctggcgcat ggcgcttgcc atgtcgttgt agcgtttggt
      121 gtacatctcg gccgtcagcg tccccttgcc gaacggcaac tccgaacccg cttcccacgc
      181 gccgcccagc tcgttgccga tctcccacac gcgcacgttg tagcccatct tctgattcgc
      241 ccacttgacc cactccgcgg catcgatcgg ccgacccgtg ccggcattgg gacaggcgag
      301 cggctcgacg cccggcaatg ccttcacgaa atcctgcagc gccttcacgt cgacgtggcc
      361 gtgccagttg ttgccggcgg ggtgcagcgt cttggaatcg gcaagaaagg agggcgcgta
      421 gtcgctgtaa tccacggccg gatattcatc cggccccacg cgatttggat cgaccacacc
      481 gtccaagccg cgcacgccgt ggccgttcca gtagacgagg ttggaccagg acccacccgg
      541 caggcggatg taaccgggat gcagatcgtt catccactcg cgcacctgcg gcgacgcgaa
      601 caccgttgcg aaataccaca tcgcaacgtt cgtcccgccc agccgccgcg ggctcatcgt
      661 gcgcagcgtc tgcccggcgt ccacggcgac ggtgacatcg atcgtcgaat ggttgtcgta
      721 gcgaatgcga tcgggctttg gaaagtcgaa ttcggtggtg ccgcgccggt ccggcggctc
      781 cgcccggaca tcaatgctcg caacggcgac ggcgatcagg gctgccagac agcccaacgt
      841 tgaatgcagg cgcatggggt gatcctccac ccgtgccaga atagccggcg gtcccggtat
      901 cgcttgtcgt gatacgtcaa agtagttgtt tcagtgcgcc agcatcgcaa agaaggcttc
      961 gagcaccctt atcgtgacgc gagcctccgg cccgtgctcg gtggttgata ctggcagaac
     1021 cttggtcgtt ccaagtccag tgcatcacgc acgggccgga cgcccgtatc acgatgaggt
     1081 cttcaaactt ctgaacccgc tttttacggc gcgacgccgc gggagcggac ttttcttcga
     1141 taggcctgcg gcgagatgcc tgtcgccagg cgaaagctgt ggctgagcga cgacagacag
     1201 gaaaacccgc actgctcggc gatgtccgtc agcggcaggt gcgtgcgctc gatggtatgc
     1261 ttcaccgcct ccaggcgcac ccgccgaatc tcatctcccg gtggccggcc gacgacctgc
     1321 gcgaaacgcc gttccagcgt gcgccgcgac aggtggatgc tgcggaccac atcgtcgatc
     1381 gtcgcgccgt tgcacgcctc gcggcggatg tacgcgatgg cggcggcgac gtccgggtcc
     1441 tcgtgcacgt acgtcgaggt ggattcgcgc tcgatcacac cctgcggacg aaagcgctga
     1501 tccacgcggg catcgagacg gcccatgaac gcctgatcga gcagccgggc cgcctcgcga
     1561 ccgatgccga agcgctcgat gctgatgctg ctgagcggcg gctgaacgcc gatgcactcg
     1621 gtgatgcggt catccacgcc gatcacgcct acctccgcgg ggatttgcca acgggcggcg
     1681 ttcaccgcgc gcaccacgct ggcggcgatg aggtcgttgc atcccatgac ggccatcggg
     1741 cgggcgaacc gccgcagttg ccgcgcgatc catgcctccg atgtcagctg aatccagtcg
     1801 ctggcgaaga tcagatggca ctcgcgctgc gcgctggtga atgcctgttg aagcccgccg
     1861 gcgcgctcgc ggctccaggc ggcgcgcgcg ccaaggaata tcgcccgggt gaagccgcgc
     1921 tccagcaggt gttcgcccgc cagctggccg atatagcggt tgtcgaggcg gatgcgcgcg
     1981 tagggcgcgc ggtcggcgtc accgtccact tcgatgacct tgacgccccc ctcgtgcagc
     2041 gggcgaagcg tgcgccagcg accgggcatc atgatcacgc catcgaccac ggtcttgctg
     2101 agccgccgct ggcgccccgg cgtgacgtgg aacgggatcg ggatgctgcg caatccccag
     2161 ctgccgctgc gcgggcgata gctggccacg ccgcggcaga agtcccacgc ttcgagcgtg
     2221 tcgtcggtgt gtataatcgc gacgcgatgg gtgacggaag accgcagggg agggggggac
     2281 atcggatgtg aacgtcaagc tatccgatcg caagctggaa catcaagtgg cggaacgcga
     2341 caaatgcttt gtcgcaatgt ggcaatcgaa agctgaccct gcgcggtatg ctgggaagct
     2401 atgcggcgag gaacccggcg gccttggcgt ccccttgatc tgatgaccgt ttcacttggc
     2461 ctgctgctga tgcggctcat gacgccggca gcctgggcgg ggtcgtctgg agccgcggct
     2521 tcgcccgcgt ccgataaccc tgcgccgccc gatgcctggg tggccgtcgc gcagatgacg
     2581 ccgggcatca acatcggcaa cacgctcgag gccaccgcgg gctgggaaac cggttggggc
     2641 aatccgccga tcacgaagga atacgttcaa acactagcgc ggctgggatt caaaacggtg
     2701 cgcctgccgg tcgcctggga cacgtacgcc gacaacggcc gcatcacgca gaagcaattc
     2761 gatcgcgtcg ctgaagtcgt c
//
LOCUS       DATIMH010000836         1637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2219276, whole genome shotgun sequence.
ACCESSION   DATIMH010000836 DATIMH010000000
VERSION     DATIMH010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219276"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="VLI90_14495"
     CDS             <1..1552
                     /locus_tag="VLI90_14495"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSV15466.1"
                     /translation="PGFQLVALDAKSGVPVARFGQSGIVDLFTQLDNDLKIDPLAKIG
                     NSSPPVVVGDVVVVGPALLVGTRVNKSNVRGDVLAFDVRTGKKVWEFHTVPQPGERFN
                     ETWRDVADERMGAGRGWYHRGGTNMWAFSASIDAERGIVYLPIAGPAANYYGGDRPGN
                     NVYGNSVVAVDAETGAYKWHFQTVHHDLWDSDTPSAGGLFNVRVNGRTIPVIASVGKT
                     SYMFILDRTTGKPVFGVEEKRVPKGDVPTEWYSPTQPIPLKPRPLSRVSFKPEDMVTA
                     ADTTPEHAEACKAMWDAAGGYRNDGPFTPFYFHEKGAPPKSTIQFPGGIGGVNWGGVA
                     VDPTTGYVFVQAHDTSLVGWTEVKEDGNYSFDAPGSTQAYDRASVAGKGPFFTFNASV
                     TGKTGGASLPCQKGPWAKLVAVNANTGDIAWESVLGLNESLPEGKQLSGNAGSAGPTV
                     TAGGLVFIGATNDRRFRAFDSKTGKEIWTMPIAANANANPMSYQGKSGKQYVAIVAGS
                     NVVAFALP"
BASE COUNT          295 a          538 c          552 g          252 t
ORIGIN      
        1 gcccggtttc cagctggtcg cgctcgatgc gaagagcggc gtgcccgtgg cccgcttcgg
       61 ccagagcggg atcgtcgatc tgttcacgca gctcgataac gatctgaaga tcgatccgct
      121 cgccaagatc ggcaacagct cgccgccggt ggtcgttggc gacgtcgtcg tcgtcggtcc
      181 ggcgctgctg gtcgggacac gcgtcaacaa gtcgaacgtg cgtggcgacg tgctggcatt
      241 cgacgtgcgg accggcaaga aggtgtggga gttccacacg gtgccgcagc ccggcgagcg
      301 cttcaacgaa acgtggcgcg acgtcgccga cgagcgcatg ggtgcgggcc gcggctggta
      361 ccaccgcggc ggcacgaaca tgtgggcgtt ctccgcgtcg atcgatgcgg aacgcggcat
      421 cgtgtacctg ccgatcgccg gtccggccgc gaactactac ggcggagatc gtcccggtaa
      481 caacgtctac ggcaattcgg tcgtcgccgt ggatgccgag accggcgcct acaagtggca
      541 cttccagacc gtgcatcacg atctgtggga ctcggatacg ccgtcagcag gcggactctt
      601 caacgtgcgc gtcaacgggc gcaccattcc ggtaattgcg tcggtgggga agacgagcta
      661 catgttcatc ctcgatcgca ccaccggtaa gccggtcttc ggcgtcgagg agaagagagt
      721 gccgaagggc gacgtgccga ccgaatggta ctcgccaacg cagccgatcc cgctgaagcc
      781 gcggccgctc agccgcgtca gcttcaagcc cgaagacatg gtcaccgcgg ccgacaccac
      841 tcccgagcac gctgaggcct gcaaggcgat gtgggatgcc gcgggcggct accgcaacga
      901 cgggccgttc acgccgtttt acttccacga gaagggcgcg ccaccgaagt ccacgatcca
      961 gttccccggt ggcatcggcg gcgtgaactg gggcggcgtc gctgtcgatc cgacgaccgg
     1021 ctacgtcttc gtgcaggcgc acgacacgtc gctggtgggc tggaccgagg tcaaggaaga
     1081 cggcaactac agcttcgacg cgcccggatc cacgcaagcc tacgaccgcg ccagcgtggc
     1141 ggggaagggg ccgttcttca cgttcaacgc atcggtcacc ggcaagacgg gcggagcgtc
     1201 gctgccgtgc cagaagggtc cgtgggccaa gctggtggca gtgaacgcga acacgggcga
     1261 catcgcgtgg gaatcggtcc tcggcctcaa tgaatcactg cccgaaggca agcagctgtc
     1321 cggcaacgcc ggcagcgccg gcccgaccgt gaccgccggc gggctggtgt tcatcggcgc
     1381 caccaacgat cgccgcttcc gcgcgttcga ttcgaagacc ggcaaggaga tctggaccat
     1441 gccgatcgcc gcgaacgcca acgccaaccc gatgtcgtac cagggcaaga gcggcaagca
     1501 gtacgtcgcc atcgtcgccg gcagcaacgt cgtcgccttc gccttgccgt aggaggagaa
     1561 cccatgcgga ctcgtcgcca gaccgccttt gccgctgttg tcgccgcggc aatggcgatc
     1621 gtcaccgttg cggcgca
//
LOCUS       DATIMH010000837         5291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1067479, whole genome shotgun sequence.
ACCESSION   DATIMH010000837 DATIMH010000000
VERSION     DATIMH010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067479"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..682
                     /locus_tag="VLI90_14500"
     CDS             71..682
                     /locus_tag="VLI90_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV15467.1"
                     /translation="MMRSRRTRRAFTIIEAALSVAIVGVLLATSMTTFAAIAKQRKVQ
                     MERRLGYELGQQLMSEILQQYFQDPVSPVFGPESGETRANYDDVDDYNGWTETPPARR
                     DGTALSDYSGWTRAVAVAYVDPLNPGSTIGSSTLKRVTVTVTAPSGKQYALAGYRSQY
                     GPYEYIPPAVTSYLTWAGVDLQVGTTTKNVRTGARPLNITASQ"
     gene            751..2025
                     /locus_tag="VLI90_14505"
     CDS             751..2025
                     /locus_tag="VLI90_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15468.1"
                     /translation="MSRHRRGGAYILVLGVAMLVTVIGMAALMTTRLAAREANSSTDW
                     EEAGTLAQAAVEHAISYLNAKVTANPTGWRAAFTSYTPGSGVYAFTQSAGRGTFNWAV
                     KDEVDGNFSNNYVDPFRLYGIGKVGLATRVYSVQIVPAGSPLDVLRCAVHANGNVTQS
                     GVICCGSNAAGYTGPLSSNAKISFTNFVYGNVEAVTTSGANSLVKGTLTTPAAVKTMP
                     SPGVYNLYLNKATVIPWSALSNSIAPGLLSSTYNPYGTTNPDGVYSITVPAAQSLTIS
                     SSRIKGTLLISMSGGSLNLTGPILWEPNRTDFPILIINGSGVSVSISGSTTWLSESAV
                     GMDLDGDNLTTHDLPPQYRGLIHIIGSTNTVMVSNNAYIRGLLLTDGALSTSGQTTFV
                     SDSTLYTKPPIGYGLGDQLMIVPGSWVWDSTP"
     gene            2305..2727
                     /locus_tag="VLI90_14510"
     CDS             2305..2727
                     /locus_tag="VLI90_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSV15469.1"
                     /translation="MGSKLYVGNLSYNTTSSDLEQLFAQHGSVQSAEVISDRETGRSK
                     GFGFVQMGSDEEAQAAIAALNGQEHDGRPLTVNEAKPREDRPRGGGGGGGGRSFGGGG
                     GGGGRGGYGGGSGGGGGGRGGYGGGGGGGGGRDRGNRY"
     gene            2973..4883
                     /locus_tag="VLI90_14515"
     CDS             2973..4883
                     /locus_tag="VLI90_14515"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HSV15470.1"
                     /translation="MARTIARRLFPVCIAFVLFTVGCNSGPAPRPAGKGLGGIYDVRA
                     FGAAGDGATLDTNAVNSAIDAAEADGGGTVHFPAGVYLCHSIHLKSNVALYLDQGSTL
                     MAAPPAPKGSDALTYDVPEPNPSDHYQDFGHTHWHNSFIWGVNLRNIAIMGPGMIDGS
                     KGLVRGGVDPKKKEDPKVGAGMGAGDKPTTEPTSKPTSKPTTKGARKPREPRKPKPEF
                     EYPTERDSVRPGLANKAIALKLCTNVILKDFSIRAGGHFGILATGVDNFTLDGLKIDT
                     NRDGMDLDCCTNTRVANCTVNSPFDDGICPKSSYALGYNKSTDNMTITNCQVSGYVAG
                     SLLDGTCKPFWGKNAKSPGGTGRIKCGTESNGGFRNLSITNCVFDYCRGFALESVDGA
                     LLEDVTVSNLSMRHIANSPIFMRLGARLRGPKQTTQVGTLQRVNIDNVVVYDADPNFA
                     SIISGIPDHDIKNVKISNVQVFTRKADAEKTKKYATTQSLEFVRKYPEPGMFGPTGSY
                     GFFVRHVSGIEFNNVSVHNGGDDYRPAFLLTDVTGAKLVDVDTDHDASVAPFILRNAK
                     DVYVHQCAGVDDTRLDSIDNGKLGSGGIPTLDKKKPEPRDDQTDEDYQAQIKRLEKGS
                     DPATIPADDDQE"
     gene            5080..>5291
                     /locus_tag="VLI90_14520"
     CDS             5080..>5291
                     /locus_tag="VLI90_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15471.1"
                     /translation="MRIASSVLVIALLLLAVPARAADSTDIADPTSAPAAHPKLDPSL
                     PTLFLIGDSTVKNGHDNGAGNMYGWG"
BASE COUNT         1090 a         1624 c         1690 g          887 t
ORIGIN      
        1 gcgatggttc gacgtggtcg agcacgtcga cgaaacagtg tctgcgcctt tacgtctacg
       61 gcaccaccca atgatgcgaa gccggcgaac aagacgcgcg ttcacgatca tcgaggcggc
      121 gttgtccgtc gcgatcgtcg gtgtgctgtt ggccacgtcg atgacgacct tcgccgccat
      181 cgccaagcag cgcaaggtac agatggagcg gcggctgggt tacgagctgg gccagcagtt
      241 gatgagcgaa atcctgcagc agtatttcca ggatccggtc agccccgtct tcggccccga
      301 gtcgggcgag acgcgggcga attatgacga cgtcgatgat tacaacggat ggactgagac
      361 gccgccggcg cgacgggatg gcacggcgct gagcgactac agcgggtgga cccgcgcggt
      421 ggcggtggcg tatgtggatc cgctgaatcc gggcagcacg atcggaagct cgacactgaa
      481 gcgcgtgacg gtgacggtga ccgcgccgag cgggaagcaa tatgcgctgg cggggtatcg
      541 ctcgcagtat gggccgtacg aatacatacc gccggcggtg acgagctacc tgacgtgggc
      601 gggtgtggat ctgcaggtgg gaacgacaac caagaacgta aggacagggg cgcggccgtt
      661 gaatatcacg gcgtcgcagt gacagccgct tgcggcttcg caaacgatcg accgctggat
      721 tgcgaagccg tgagcggcga aagggatcag atgtccagac atcgccgcgg tggcgcatac
      781 atcctcgtgc tgggcgtggc gatgctggtc acggtcatcg gcatggccgc gctgatgacg
      841 acgcggctgg cggcgcggga ggcgaacagc tcgaccgact gggaagaagc cggcacgctg
      901 gcgcaggcgg cagtcgagca tgcaatctcg tatctcaacg cgaaggtcac ggcaaatccg
      961 acgggttggc gtgctgcgtt caccagttac acgcccggtt ccggcgtcta tgcgtttacg
     1021 caatcagccg ggcgcgggac gttcaactgg gccgtcaaag acgaagtcga cggaaatttc
     1081 tcgaacaatt acgttgaccc gttccggctc tacggtatcg gaaaagtcgg cctggcgacg
     1141 cgcgtgtaca gcgtgcagat cgtcccggca ggctcgccgt tggacgtgct gcgctgcgcc
     1201 gttcatgcca acggcaacgt gacccagagc ggtgtgatct gctgtggatc caacgccgcc
     1261 ggctacacgg ggccgctctc cagcaatgcc aagatcagct ttaccaactt cgtctatgga
     1321 aacgtcgaag ccgtcacaac gagcggggca aacagcctgg tgaagggaac actgacgacg
     1381 cccgccgccg tcaagacgat gccgtcgcct ggcgtgtaca acctctacct caacaaggcg
     1441 acggtgattc cctggtcagc gctcagcaac agcatcgcgc cgggattgct cagttcaacg
     1501 tacaatcctt acggtacgac gaacccggac ggggtctatt cgatcaccgt gccggcggcg
     1561 cagagtttga cgatcagctc cagccgcatc aaggggacat tgctcatctc gatgagcggg
     1621 gggtcgttga acctcaccgg tccgattttg tgggaaccga accgcacgga ctttccaatt
     1681 ttaatcatca acgggagcgg ggtgagcgtc agcatcagcg gatcgaccac gtggctgagc
     1741 gagtcggcgg tgggaatgga tctggacggc gacaatctca ccacgcacga cctgccgccg
     1801 cagtaccgcg ggttgattca catcatcggc tcgaccaaca cggtaatggt atcgaacaac
     1861 gcatacatcc gggggctgtt gctgacggac ggggcattga gcaccagcgg gcagacgacg
     1921 ttcgtctcgg actccacgct atatactaaa ccgccgatcg gctacgggtt gggcgatcag
     1981 ctgatgatcg taccgggctc gtgggtgtgg gattccacac cgtagtcaag ttctttcccc
     2041 ctctcccggc gtaccgggcg aggggaccgg agcagcatgg agcagcaatc tagacgcgcc
     2101 gaaaagaatc tgttacagtg gccattggta agttcgacga ccgagtccgc cgcaggcgcg
     2161 caggcacatg ttcttcagcc cgatccgcca cgtgtcgctc tctcgcccgc tcctaccgtt
     2221 tgggccgaac cgttggcccc tttcatctcg tagacccgga aagtaaaagg cgggtccgat
     2281 aggcccatcg acaggagata cctcatgggc agcaagctgt atgtcggaaa tctgagctac
     2341 aacacgacga gctcggatct ggagcaattg ttcgcgcagc acggcagcgt gcagagcgcg
     2401 gaagtcatca gcgaccgcga gaccggtcgc agcaagggat tcgggttcgt ccagatgggt
     2461 tctgatgaag aagcccaggc ggcgatcgcg gccctgaacg gtcaggaaca cgacggccga
     2521 ccgttgaccg tcaacgaagc caagccccgc gaagaccgcc cccgtggcgg tggtggcggt
     2581 ggtggtggcc gcagcttcgg tggcggcggt ggtggtggtg gccgcggtgg ttacggcggc
     2641 ggcagtggtg gtggcggcgg tggccgcggt ggatacggcg gtggtggtgg tggcggcggt
     2701 ggccgcgacc gcggcaaccg gtattgatcg ctgctcgcag ctcaatccag attgaaaagc
     2761 agacgggagg tcaccggaag gtggcctccc gttgttttag gtggttgccg cttgcggctt
     2821 cgcagaccac accgcttggt tcgcgaagcc gcaagcggcg aataaatgcg ctcaattcaa
     2881 ccattgcatc gggccgcggt aacatcaata ctgtcacccc aacttaggct ggcagtacgc
     2941 tcgaccaccc ctttctcggc ggacctcgaa ccatggcacg caccatcgct cgtcggttgt
     3001 tccccgtctg catcgcattt gtgttgttca ccgtgggctg caacagcggc ccggcgcccc
     3061 gcccggcggg taagggactg ggcggcatct atgatgtgcg cgccttcggc gcggcgggcg
     3121 atggcgcgac gctggacacg aatgccgtca acagcgccat cgacgccgcc gaagccgatg
     3181 gcggcggcac cgtgcacttc ccggcgggcg tgtacctgtg tcactcgatt catctcaaga
     3241 gcaacgtcgc gctgtatctg gaccagggct cgacgctgat ggccgccccg ccggcaccga
     3301 agggcagtga cgcgctgacg tacgatgtgc ccgagccgaa cccgtccgat cactaccagg
     3361 attttggcca cacgcactgg cacaacagtt ttatctgggg cgtgaacctg cggaacatcg
     3421 ccatcatggg gccgggcatg atcgacggca gcaaaggcct ggtccgcggc ggggtggatc
     3481 cgaagaagaa ggaagatccg aaggtgggcg ctggcatggg tgcaggcgac aagccgacga
     3541 ccgagccgac gtccaagccc acatcgaagc cgacgaccaa gggtgcgcgc aagccgcgcg
     3601 agccgcgtaa accgaagccg gagtttgaat atccgaccga acgtgattcg gtccgtcccg
     3661 ggctggctaa caaggccatc gcgctcaagc tctgcaccaa cgtcatcctc aaagacttca
     3721 gcatccgcgc cggcggacac ttcggcatcc tcgccaccgg cgtcgacaac ttcacgctcg
     3781 atggcctgaa gatcgacacc aaccgcgacg gcatggacct ggactgctgc acgaatacgc
     3841 gcgttgccaa ctgcacggtc aactcgccgt tcgacgatgg catctgcccc aagagcagct
     3901 acgcgctggg ctacaacaaa tcgaccgaca acatgacgat caccaactgc caggtgagcg
     3961 gctacgtggc cggttcgttg ctggacggca cctgcaaacc gttttggggt aagaacgcca
     4021 aatcgcccgg tgggaccggg cgaatcaagt gcggcaccga gtccaacggc gggttccgca
     4081 acctgagcat caccaactgc gtcttcgact attgccgcgg gttcgcgctg gaatcggtgg
     4141 acggcgcgct gctggaagac gtgacggtgt cgaacctgtc gatgcggcac atcgccaact
     4201 cgccgatctt catgcggctc ggggcgcgcc tgcgcggccc gaagcaaacc acgcaggtcg
     4261 gcacgctgca acgcgtgaac atcgacaatg tcgtcgtcta cgacgccgat cccaacttcg
     4321 cctcgatcat cagcggcatt cccgatcatg acatcaagaa cgtgaagatc agcaacgtgc
     4381 aggtcttcac ccgcaaggcc gacgcggaga agacgaagaa atatgcgacg acgcagtcgc
     4441 tcgaattcgt ccgcaaatac cccgagcccg gcatgttcgg gccgaccggg tcgtacggct
     4501 tcttcgtccg ccacgtcagc gggatcgaat tcaacaacgt ctcggtgcac aacggcggcg
     4561 acgattaccg cccggcgttt ttgctgaccg acgtcaccgg cgccaagctg gtggacgtcg
     4621 acaccgacca cgacgccagc gtcgccccgt tcatcctgcg caacgcgaag gacgtctacg
     4681 tccaccaatg cgccggcgtc gacgacacgc ggttggattc gatcgacaac ggcaagttgg
     4741 gcagcggcgg gatcccgacg ctggacaaga agaaacccga gccgcgggat gatcagacgg
     4801 atgaagatta ccaggcgcag atcaagcggc tggaaaaagg atcagacccc gcgacgatcc
     4861 cggcggatga cgatcaggag tgaggcaatc tttatcctga gcgaaggacg cgcagcgtcc
     4921 gcagtcgaag gatctagcca agaacgtcgg tgaaccgcgt ttcgcaaaag cccaaacgaa
     4981 aaatgcggcg cattccgcca caacctcgat cggaatcgta ggtggcccct acaacggcct
     5041 tcgcatacgc tggtaatctt ccagcatcag gaggatttca tgcgcattgc atcgtccgtt
     5101 ttagttatcg ctctgttgct cctcgccgtc cccgcgcggg ccgccgacag cacggacatc
     5161 gccgacccca ccagcgcgcc ggccgcccat cccaagctcg atccgagcct gcccacgctg
     5221 tttctgatcg gcgactcgac cgtcaagaac ggccacgaca acggagccgg caacatgtac
     5281 ggctggggca a
//
LOCUS       DATIMH010000838         6040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3949687, whole genome shotgun sequence.
ACCESSION   DATIMH010000838 DATIMH010000000
VERSION     DATIMH010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3949687"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2817)
                     /locus_tag="VLI90_14525"
     CDS             complement(<1..2817)
                     /locus_tag="VLI90_14525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSV15472.1"
                     /translation="MMRRRTEGSVRVGAGKDAADPASGGAGAGGGPRISPRRIAFDYI
                     GAAIGTAVALALMALLRHVHDVDAPFLLALVPVVFGSWLGGLGPGLFAATLVTLGIDF
                     FFEPPLYTFQIQSYGQGLNLSVFLLESASVVWLIEAVRAERRRAERHAQESEDHERAL
                     IQSEMRFKQLADSGTIGVVSCDLAGTILDANETFLQLIGYSRQDLTARAITWETLTPP
                     QQRERDALAMQQLKAKGMCTPYEKSFARKDGTLVPVLLGATWPNQFSDRIHAYVVDLT
                     GRQRAEEMAASSARRLADTLENLGDAFVMLDEHRRFTYVNREAERLLEHSSQELLGRT
                     IWSILPLLEESPLRRGFDRAAEQHITVRFEQYIEAADLWYAVHAYPIEHGLAVYFHDM
                     TRLRQSEAALRESEQRLRISRERLDLVVQSSGLGLWYCDLPFDELAWNDTCREHFGVA
                     LDERITMERFFDRLHPDDRDRTREAIDRAVRDHETYDIDFRTVAPDGRMRWLRAIGRA
                     FYDDAGNARRFDGITMDVTERRASEEALRRSEAQLRFAIAAAHMGDWSWDIETGFVIR
                     SASTQRLMGFEPGEMEPHIERFHGCVHPEDSAAVRAAMDQAIAENVPYEMEFRIVRPN
                     DAAVRWMRDRGEVFRDDNGKSLQMAGIIVDVTDRKQAEVELQRAKEEAESANRSKDEF
                     LATLSHELRTPLNAILGWAQILQTSGRDREDVAEGLEVIERNAKAQAQLIEDVLDVSR
                     IISGKIRLDVKPIALVPVIDAAMAAVRPAAEAKEIRLHSILDPSASPVLGDAGRLQQI
                     VWNIVANAIKFTPRGGRVDVQLSRRNGEGPMAELTITDTGQGIKAEFLPHVFERFRQA
                     DASTTRQFGGLGIGLSIVKQLTEMHGGSVRAESPGKGRGATFIVTLPVRAIEEPDQDA
                     GPDTLQPRHARSAADGPASTCDGAN"
     gene            complement(3129..4331)
                     /locus_tag="VLI90_14530"
     CDS             complement(3129..4331)
                     /locus_tag="VLI90_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSV15473.1"
                     /translation="MPVVYLILLAVGLGDVAWWRWADRRSTVLPRRVVWRTLIALFIG
                     SQLVYLAYFVAAPKSARRVHPWLPMPFIAMVYVWHLLVLPASLIGVGASKVVSGTKRL
                     LTQIAHGSAVGPVVSRRQAILGGAAVAAPPLLTLAGVARAVPQLDHFRIRPMDLRVPG
                     LPPNLDGLRIAHVSDIHVGRYIRRGLLPRIVEATNQLKADLVLFTGDLIDLSLADLDR
                     GIEVMKQLDPRQGLAIIEGNHDLIEDPDAFDGRMKAAGLPLLVDEAMTIAVRGERVQL
                     LGMRWGTPGADRRRHAGEASFRASLDRLLPRRESGAFPIVLAHHPHAFDMVAAAGLPL
                     TLSGHTHGGQLMLSEAVGFGPVFFRYWSGLYRKGDSQLVVSNGVGNWFPLRVQAPAEI
                     IALTLHAA"
     gene            5536..5994
                     /locus_tag="VLI90_14535"
     CDS             5536..5994
                     /locus_tag="VLI90_14535"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSV15474.1"
                     /translation="MHEPWLIMSFIAGVLLLSSLRATAAENSTTRPTTLPSQVFNWNE
                     MAAKTTAAGERRALTQLPTATLDELEMHITTLNPGQQAHAPHRHPHEELILLKEGTLE
                     AMINDQLIPMPAGSVLFIAPNDLHVVKNVGTTPATYFIVTWKTPLTEKAK"
BASE COUNT         1092 a         2073 c         1814 g         1061 t
ORIGIN      
        1 gttcgccccg tcgcaggttg aggcggggcc gtcggccgcc gaccgggcgt gccggggttg
       61 aagggtgtcg gggccggcgt cctggtccgg ctcctcgatg gcgcggacgg gcagggtgac
      121 gatgaacgtc gcgccccggc ccttgccggg gctctcggcg cggacggagc cgccgtgcat
      181 ctcggtcagt tgcttgacga tcgacaggcc gatccccagc ccgccgaact gccgcgtcgt
      241 cgaggcgtcc gcctggcgga accgctcgaa cacgtgcggc aggaactccg ctttgatccc
      301 ctgccccgtg tcggtgatcg tcagctcggc catcggacct tcgccattgc gccggctcag
      361 ctgcacatcc acgcgcccgc cgcgcggcgt gaacttgatc gcgttggcga cgatgttcca
      421 cacgatctgc tgcaatcgtc ccgcgtcgcc cagcaccggg ctggccgatg gatcgaggat
      481 cgaatgcagc cggatttcct tcgcctccgc cgccgggcga acggcggcca tcgcggcatc
      541 gatcaccggc accagcgcga tcggcttcac gtccagccgg atcttgcccg agatgatgcg
      601 cgagacgtcc agcacatctt cgatcagctg cgcctgcgcc ttggcgttgc gctcgatgac
      661 ctcaagccct tcggccacgt cctcgcgatc gcgcccgctc gtctgcagga tctgcgccca
      721 gcccaggatc gcattcaacg gcgtgcgcag ctcgtggctc agcgtcgcca ggaattcgtc
      781 cttgctgcga ttggccgact ccgcctcttc cttcgcccgc tgcagctcga cctccgcctg
      841 cttgcggtcg gtcacgtcca cgatgatgcc cgccatctgc agcgattttc cgttgtcatc
      901 gcggaatact tcgccgcggt cgcgcatcca ccgcacggcg gcgtcgttgg gccggacgat
      961 gcgaaattcc atttcgtacg gcacgttctc cgcgatcgcc tggtccatcg ccgcgcggac
     1021 ggcagcgctg tcttccggat gcacgcagcc gtggaatcgc tcgatgtgcg gctccatctc
     1081 gcccggctcg aaccccatca accgctgggt actggcgctg cggatgacga acccggtctc
     1141 gatgtcccaa gaccaatcgc ccatgtgggc cgcggcaatc gcgaagcgca gctgcgcttc
     1201 gctgcggcgc agcgcctctt ccgacgcccg ccgctcggtc acgtccatcg tgatgccgtc
     1261 gaagcgccgg gcgttccccg cgtcgtcgta aaacgcccgg ccgatcgcgc ggagccagcg
     1321 cattcgcccg tccggcgcca ccgtgcgaaa gtcgatgtcg taggtttcgt ggtcgcgcac
     1381 cgcgcgatcg atcgcctcgc gcgtgcgatc acgatcgtcc ggatgcagcc gatcgaaaaa
     1441 acgctccatc gtgatccgct cgtccagcgc aacgccaaaa tgctcccggc aggtgtcgtt
     1501 ccacgccaac tcgtcgaagg gcagatcgca ataccacaac cccagccccg acgactgcac
     1561 gaccaggtcc aagcgctccc ggctgatgcg cagtcgctgc tcgctctcgc gcagcgccgc
     1621 ctctgattgc cgcagccgcg tcatgtcgtg gaagtaaacg gccaagccgt gctcgatcgg
     1681 atacgcgtga accgcgtacc acaggtcggc ggcctcgatg tactgctcga agcgcaccgt
     1741 gatgtgctgc tcggccgccc ggtcgaaccc gcggcgcaac ggcgattctt ccagcaacgg
     1801 caggatcgac cagatcgtcc gcccgagcag ctcctggctc gaatgctcga gcaggcgctc
     1861 ggcttcgcga ttgacgtacg tgaaccgccg gtgctcatcg agcatgacga acgcgtcgcc
     1921 gaggttctcc agcgtgtcgg ccagccgacg cgccgacgat gccgccattt cctcggcgcg
     1981 ctgccgaccc gtcagatcga cgacgtaagc gtgaatgcga tcgctgaatt ggttcggcca
     2041 cgtcgcgcca agcagcaccg gcaccagcgt gccgtctttg cgcgcgaacg atttttcgta
     2101 cggcgtgcac atgcccttgg ccttcaactg ctgcatcgcc agggcatcgc gctcgcgctg
     2161 ctgcggcggt gtgagcgtct cccaggtgat cgcccgcgcc gtcaagtctt gccggctgta
     2221 tccgatcagt tgcaggaacg tttcgttcgc gtcaaggatg gtgccggcca ggtcgcagga
     2281 gaccacgccg atcgtgccgg agtcggccag ctgcttgaag cgcatctcgc tttggatgag
     2341 cgcgcgttcg tggtcttccg attcctgcgc gtgccgctcg gcgcgccgtc gttcggcgcg
     2401 aaccgcttca atcaaccaaa ccacgctggc ggattcgaga aggaagacac tgagattgag
     2461 cccctggccg taactttgga tttgaaaggt gtacagcggc ggttcgaaga aaaagtcaat
     2521 gccgagcgtg actagcgtgg cggcgaacag ccccggtccc aatccaccca accaggagcc
     2581 gaacaccacc ggcaccagcg ccagaaggaa tggcgcatcg acatcgtgta cgtgccgcag
     2641 cagggccatc aatgccagcg ccaccgcggt gccgatggcc gcgccgatgt agtcaaaggc
     2701 gattcgccga ggcgaaatcc gcggcccacc ccccgcgccc gccccgccgc tggcgggatc
     2761 cgcggcatcc ttgccagccc ccacgcggac cgatccttcg gttcgtcgtc gcatcatgaa
     2821 acatcccatt ccagcgcggc tccaggcgca gcgcttttgg aagacttacg atttcctgct
     2881 ctgacgcgta cacacgatct gatttcgccc ggtaacggat tgcgggccgc attctaccgc
     2941 gaccccccaa acaggtcacg cccatcccgc cgttgacgac agttttaatt ttccttagtc
     3001 gcgctgaaga aaatgcgaag aatctgaacc acagattttc acagattgcg cagatccgga
     3061 agaccttctc tgcaatctgc gtcatctgtg aaatctgtgg tttcaaagct tctgcttgcc
     3121 agcatctgct acgccgcgtg cagcgtcagc gcgatgatct cggccggcgc ttgcacgcgc
     3181 agcgggaacc agttgcccac gccgttcgat acgaccagct ggctgtcgcc tttgcgatat
     3241 aagccggacc agtagcggaa gaagacgggg ccgaagccga cggcttcgga cagcatcagc
     3301 tgtccgccgt gcgtgtggcc ggacaacgtc agcggcaatc ccgccgccgc gaccatgtcg
     3361 aacgcgtgcg gatgatgcgc caagacgatg gggaacgcac ccgactcgcg ccgcggcaac
     3421 agtcgatcga gcgaggcgcg aaacgacgcc tcgcccgcgt gtcgccggcg gtcggcgccg
     3481 ggggtgcccc agcgcatgcc gagtagctgt acgcgctccc cgcgcactgc gatggtcatc
     3541 gcctcgtcca ccagcagcgg caagccggcc gccttcattc ggccatcgaa cgcgtcgggg
     3601 tcttcgatca ggtcgtgatt gccttcaatg atcgccagcc cctgccgcgg gtcgagttgc
     3661 ttcatcacct cgatcccgcg atcgagatcc gccagcgaca ggtcgattag gtcgccggtg
     3721 aacaggacca ggtccgcctt cagctggttc gtcgcctcga caatccgcgg caacaatcca
     3781 cggcggatgt accggccgac gtggatgtcg ctgacgtgcg cgatgcgcag cccatccaaa
     3841 ttcggcggca ggcccggcac acgcaggtcc atcggtcgga tgcggaagtg atcaagctgc
     3901 ggcaccgccc gcgcgacgcc ggcgagggtc agcaacggtg gtgcggcaac ggctgcgccg
     3961 ccaagaatag cttggcggcg cgagacgacc ggtcccacgg cggagccgtg ggctatttgg
     4021 gtgagtaacc tcttcgtacc gctcacgact ttcgacgcgc cgacgccgat caaacttgcc
     4081 ggcagcacca gcaggtgcca cacgtacacc atcgcgataa acggcatcgg cagccacgga
     4141 tgcacccgcc gggcagactt cggcgccgcc acgaagtacg ccagataaac cagctgcgat
     4201 ccgatgaaca gcgcgatgag cgtccgccaa accacccgcc gcggcaaaac agtcgaacga
     4261 cgatccgccc agcgccacca ggcgacatcc cccagcccca ccgcgagtag aattaaataa
     4321 acaaccggca tcgcggaagt atagggcagc gatcgaaacg cgaggcgaac cgaaagaagt
     4381 tcccgatcct cgaaaccccc ttttagcggc ggcccgaagg gccgagcgtc cgcgcagtcc
     4441 gactggtcga aaaccacgac tccttattta gaccttgcct cggcgttgct tcggccgcga
     4501 tcgaccgcgc gccccgttcg cgttcagatt ccacgagccg accattctcc atcccgatga
     4561 atggagtgcc catcgccttc gacaacgccc gcgctttccg cgctgctcgc ttcaatgcgg
     4621 caagcgtcgc ctgcacgtct gcatcatgtc ccgcgcgatt cgttctcatc ggttatagcc
     4681 cctggtgatc agcttaggtg gcatcgccac attattgtag agctgccaag tgctcaccag
     4741 ctgacgatat aggctatcaa agtttcgcat accggacgcg aagcgccggc ggacgatctc
     4801 ttctgcaaca tcgtgtccac cttgtgaaac acgagctctt actcgcgcga ttgcggcgtc
     4861 cgctgagggc agttgcagga agaggagctt cacgtaacat ccctcgtccc gccagcctga
     4921 cgacccgaca cttttgggga acaaccagcg gaaccccagt ttttccatcg cccgatgcga
     4981 ggcgcatcga cgttcgccat gccgggttcg gaggtactcc gacgacccgg attcttctcg
     5041 cgcgccgcag aatctcgctt cgtcggagta cctccgaacc cggcgtggat cggttcctaa
     5101 tcacttctgc aaaagtgtcg gaccgtcagt cccgccagcg cggaatatgc ttggcgtaat
     5161 ttaatccact caacgtcgtc tcgaacgcaa aactttctcc cgcaccgacc gtcgcgctat
     5221 ttcgcccagc atgatctttc cagcctgaaa cgccacttgc tccggcccaa acggggaaag
     5281 tccacgcgcg atcaagtcgg cgttgatgaa cgcaggacag cccgcttccc gcgggagata
     5341 ctcttcggca aatgtcgtct tccccgcgcc attcggcccg gaaatgatga cgatccgctt
     5401 ctgccttctg cgggccatgc cgaggattct gccggtagtg gcatcactca gcaagttgtg
     5461 ccggcgctga gcggcttgcg acctttagtg atcgcacgca tcatgtgccg cttcctccaa
     5521 ccccaggaac ccaccatgca cgaaccgtgg ctgatcatgt cgttcatcgc aggcgtccta
     5581 ctgctgtcga gcctgcgcgc gaccgccgca gagaactcca caacccgccc gacgacgttg
     5641 ccgtcgcagg tgttcaactg gaacgagatg gccgcgaaga ccaccgccgc gggggagcgg
     5701 cgggcactga cgcagttgcc gacggcgacg ctggatgagt tggagatgca catcacgacg
     5761 ctgaaccccg ggcagcaggc gcacgcgccg caccggcatc cgcatgagga gctgatcctg
     5821 ctgaaagagg gaacgctgga ggcgatgatc aacgatcagc tgatcccgat gccggcgggg
     5881 tcggtgctgt tcatcgcgcc gaacgatttg catgtcgtga agaacgtggg gacgacgccg
     5941 gcgacgtatt tcatagtgac gtggaagacc ccgttgacgg agaaagcgaa gtgagcgcga
     6001 cccgacgccg gtgtctgagc gtactcgcga agacccgggt
//
LOCUS       DATIMH010000839         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2220244, whole genome shotgun sequence.
ACCESSION   DATIMH010000839 DATIMH010000000
VERSION     DATIMH010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220244"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VLI90_14540"
     CDS             <1..261
                     /locus_tag="VLI90_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15475.1"
                     /translation="LGEKPSIQLFAHVGNDLSKFDKTHHGPYGAYEIAKCVVQGIRDD
                     KLELANHLADDVPAFDPAKPDPVETFDVPPSPGLTNQKPLGD"
     gene            425..766
                     /locus_tag="VLI90_14545"
     CDS             425..766
                     /locus_tag="VLI90_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15476.1"
                     /translation="MFLSRKLITAAVVGLGGLSLTIGATPAPKATAAPTTQPKTIGGQ
                     PNNPGTDAQRYAEVPELRRIRKELETIDGELGKDVNDPTGYRKQARDAVRKAIDAVNS
                     EVEEYDKDKPK"
     gene            complement(1292..>3658)
                     /locus_tag="VLI90_14550"
     CDS             complement(1292..>3658)
                     /locus_tag="VLI90_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15477.1"
                     /translation="PKLQPVQNTTVMRVINTPFVLLLDPDSKQYYLKAGDAWVKAPDV
                     MGPWTDAAVAPANVVNAAAQLAAAEGPQGATTQDESPATSPPVALQFREPPPQIIVSE
                     EPAELIVTDGNPTFTPLPGNDLLYVSNTRSDVFLEVSTQRYFVLLSGRWYRAASLQGP
                     WEYVAPEKLPVAFAKIPADSAKGHVLAFVPNTQPAQEARLDAQVPQAAAPIRRDAGQD
                     LSVPYDGEPQFQPVDSGSDLTYAVNTPVPVLCVDRRYYCCYQAVWYESGFAHGPWNVC
                     VSVPRVIYTIPPSCPVYNVRYVYVYEYSPDYVYCGYWPGYTGCYVYGPTVIYGTGYHY
                     SPWYRHTYIPRPCTYGFAPAFDLNTCAWGYRPTFERFSTGGRGNGNHWIFENERQRGW
                     WGPQGFVDYRRLRERDRIHADTITVNRNRTVVAQPGQARPAMERPVVDLYRRNENARR
                     VIEPREVIRAQQFTGAGAPVSAVGANAPRTPEQPGGHTPPNLPIDSTHSGIAPTFIRV
                     VDGKIYRRAPDGWQQQKDGSWVRIDSPPPSPTPTPAPAPPARIDRGEGNGSGAGAAPV
                     GPTREGGRRPTRSGPPTAVDEPTSPPRAGEPPVRSNPAQRGTPPVRGDEPVHAGPTRD
                     NEPPPADHPLPARPQAPERTEPPTVVRPQQPTQTEPPTRTEPPTRTEPPTRVEPPVRV
                     EPPTHVEPPVRAEPPRRVEPPTRVEPPVRSEPPVRAEPPVRSEPPVRVEPPVRAEPPA
                     RVEPPVRSEPPARAEPPARVEPPARAGDGQQQDGQTGPGNTPAARPGR"
BASE COUNT          621 a         1227 c         1191 g          619 t
ORIGIN      
        1 ctaggcgaga agccgtccat ccagctgttc gcgcacgtcg gcaatgacct gagcaagttc
       61 gataaaacgc accatggccc gtacggcgcg tacgagatcg ccaagtgcgt cgtccagggc
      121 attcgcgacg acaagctcga attggcgaat cacctcgccg atgacgtgcc ggcattcgat
      181 cccgccaagc ccgatccggt ggaaacgttc gacgtgccgc cgagcccggg gctgacgaat
      241 cagaaaccat tgggcgattg atgcgctcgg cggcaccagc gacctctggc gcgaaatcca
      301 gcgtagtcaa atcagaatcg ctcagcatat cctgcaacgc gtgaagatgg ttctgtcgcc
      361 ggcgcgccca tcgcgcgatg ttgcgcccgg cgattgtttc gcacgcgagt caggagttat
      421 tgccatgttt ctttcacgca agctgatcac cgccgccgtc gtgggcctcg gcggactatc
      481 gctgacgatc ggcgccaccc ccgcgcccaa ggcgaccgcc gccccgacga cgcaacccaa
      541 gacgatcggc ggccaaccga ataacccggg caccgacgcc cagcgctatg cggaagtgcc
      601 ggagcttcgc cgcattcgca aggagctgga aacgatcgac ggcgagttag gcaaggatgt
      661 gaacgatcca accggctatc gcaagcaggc gcgagacgcc gtgcgcaaag cgatcgacgc
      721 ggtgaattca gaggtcgagg aatacgacaa ggataagccg aagtaggcga ctgcggcgtc
      781 gcttccgagg tggcaaaaga tcaaagcgca cgtggccggc gagttacgcg ccggccacct
      841 gcgttttaga gccgtgtgca tctgaagtgg ctgagtcttt aatagcccgg gccggaaggc
      901 cggggatcgg acagatagct gcgctatttt gccgtccgat cccggcctcc cggcctgggc
      961 tataaccggc gcgagcacgt actcgtgaac gagccgaaga tcttgcttcg accgtcggtg
     1021 agcctcggat gaagcaagcg tttctatcgc tgctggtcaa gatccttcga ttcccgcgag
     1081 tacgcctgcg ctcatatgtg tgcgcgcgcc gctcagctga aaatctgtcg ccagccgcgg
     1141 agataaatca cattttgaaa atcccttgat agccgcaggc aatagccagc gggggctctc
     1201 gaaatcagcg ccgctcaact ccgcgaagac ccgctggcta tcgcctgcgg ctatgaaatc
     1261 tcgcgccgac cacgaataaa tgcgacatca actaccgccc cggcctggcg gcgggggtgt
     1321 tgcctgggcc ggtctggccg tcctgctgct gaccgtcgcc cgcgcgagca gggggctcca
     1381 cgcgcgccgg gggttcggct cgcgctggag gctccgaccg aaccggtggc tcgacacgcg
     1441 ccgggggttc ggcgcgtact ggtggttcga ctctaaccgg cggctctgat cgaaccggtg
     1501 gctcggcgcg aacgggcggt tcagatctca cgggcggttc cacccgcgtc ggcggttcaa
     1561 ctcgacgcgg cggttccgca cgcaccggcg gctcgacgtg cgtcggtggc tcgacacgca
     1621 ccggcggctc aacccgcgtc ggcggctcgg tccgcgtcgg cggctcggtc cgcgtcggcg
     1681 gttccgtttg cgtcggttgc tgcggtcgca cgaccgttgg cggttcggtt cgctccggcg
     1741 cctgcggccg cgccggcagc gggtgatccg cgggcggtgg ctcgttatct cgggttggcc
     1801 cggcgtggac tggctcgtcg ccacgaaccg gcggcgtgcc acgctgcgct ggattcgacc
     1861 gcacgggcgg ctcgcccgcg cgcggcgggg atgtcggctc atcgaccgca gtcggcggtc
     1921 ctgatcgcgt cggccgccgg ccgccttctc gcgttggccc gaccggcgcg gcgccagcgc
     1981 cgctcccgtt gccttcaccg cgatcgatcc tcgccggcgg cgcgggcgcg ggcgtgggcg
     2041 tgggcgacgg tggcggcgaa tcaatcctca cccacgaccc gtctttttgc tgctgccagc
     2101 catcgggcgc tcggcgatag atcttgccgt ccaccacgcg aatgaacgtc ggggcgatgc
     2161 ccgaatgcgt tgaatcaatc ggcaggttcg gcggcgtgtg ccctcccggt tgttccggcg
     2221 tgcgcggcgc atttgcaccc accgcgctca ccggcgcacc ggcgccggtg aactgctgcg
     2281 ctcgaatcac ctcgcgcggt tcaatcacgc ggcgcgcgtt ttcgttgcgg cgatacagat
     2341 cgaccaccgg ccgctccatc gccggccgcg cctggccagg ctgcgcgacg actgtgcgat
     2401 tgcgattcac cgtaatcgtg tcggcgtgaa tgcgatcgcg ctcgcgcagg cggcggtaat
     2461 ccacaaaccc ctgcggtccc caccagccgc gttggcgttc gttctcgaaa atccagtgat
     2521 tcccgttgcc gcggccgccg gtgctgaagc gttcgaaggt cgggcgataa ccccaggcgc
     2581 aggtgttcag atcgaacgcg ggggcgaagc catacgtgca cggccgcggg atgtacgtgt
     2641 ggcgatacca gggcgagtag tggtaaccgg tgccgtagat gacggtgggc ccatacacgt
     2701 agcagccggt gtaaccgggc caatagccgc agtagacata atcgggcgag tattcgtaaa
     2761 cgtaaacgta acgaacgttg tacaccgggc agctgggcgg gatcgtgtag atcacgcgcg
     2821 gcaccgacac gcacacgttc cacggcccgt gcgcgaaccc cgattcgtac cacaccgcct
     2881 gataacagca gtaatagcgg cgatcgacac acagcaccgg caccggcgtg ttcaccgcgt
     2941 acgtcagatc ggaaccggaa tcaacgggct gaaactgcgg ctcgccatcg tacggcacgc
     3001 tcaggtcctg ccccgcgtca cgccggatcg gcgccgccgc ctgcggaacc tgcgcgtcca
     3061 gccgcgcttc ctgcgccggc tgtgtgttcg gcacgaacgc cagcacgtga cccttggccg
     3121 aatccgcggg gatctttgca aacgccaccg gcagtttctc cggcgcgaca tattcccacg
     3181 gcccttgcag cgacgccgcg cgataccacc ggcccgacag cagcacgaaa tagcgctgcg
     3241 tcgagacctc caggaagacg tccgaccgcg tgttgctgac gtacagcaga tcgttgcccg
     3301 gcaacggcgt gaacgtcgga ttgccatcgg tgacgatcag ctccgccggt tcttccgaca
     3361 cgatgatctg cggcggtggc tcgcggaact gcagcgccac cggcggcgac gtcgccggcg
     3421 attcatcctg cgtcgtcgcg ccttgaggtc cctcggccgc cgccaattgt gccgcggcgt
     3481 tcacaacgtt cgccggcgcc accgcggcgt cggtccacgg gcccatcacg tccggagcct
     3541 tcacccacgc gtcgccggcc ttcaggtaat actgcttcga atccggatcg agcagcagca
     3601 cgaacggcgt gttgatcacg cgcatcaccg ttgtgttctg caccggttga agcttcgg
//
LOCUS       DATIMH010000840         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4242379, whole genome shotgun sequence.
ACCESSION   DATIMH010000840 DATIMH010000000
VERSION     DATIMH010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4242379"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..796
                     /locus_tag="VLI90_14555"
     CDS             152..796
                     /locus_tag="VLI90_14555"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV15478.1"
                     /translation="MKIEDDLPRTRNPTTTTETPRVEADSSSREAAVDIELLRKSAAG
                     DGKAFHALVDRHADRLYRLAVSLIGNAADAEDVLQEALTGAYRGLSGFQGRASVKTWL
                     TRILVMQVAKWRRDRKRRRSGATAIDDGEADAQPAASGGATGGAGVVDARLDVHAAVQ
                     RLSAEHRQVIALREFEHLSYDEMAEVLDLPRGTIESRLHRARAELKEILKSYLP"
     gene            801..1265
                     /locus_tag="VLI90_14560"
     CDS             801..1265
                     /locus_tag="VLI90_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSV15479.1"
                     /translation="MNTCALNTKLDAYHDGELAGAELASFERHLAECGECSRQLAQLR
                     AMSDLFAAAPQVKLSQIAMHRLHQRIDVGGADQGLLRLGWTMSAIAASLLLVGSVWLT
                     RMSNPTTTPEAAPPWVGVPVVAQADPAYREIAATPAAQWYLADASTRNDEIP"
     gene            1274..1816
                     /locus_tag="VLI90_14565"
     CDS             1274..1816
                     /locus_tag="VLI90_14565"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HSV15480.1"
                     /translation="MRRTKSVIIAAFILVLSAGVVAGRLWARFPSPSATPTGKQPSWL
                     ADQLDLTPEQRQQMDAIWANVKQQMDQKLERRHALDRERDQAIEDLLGPEQWQAYGRL
                     IDEFRAKRAEIDKDRMNLIHDANDRSRALLSDVQKQRWDQMRQARHDHGGDGHDHHHG
                     DGHEPTSQRNKSDDGGNNKT"
BASE COUNT          406 a          594 c          710 g          325 t
ORIGIN      
        1 tccgcgacta tggcatgcgt cctgattcca cttcacgaaa agctgcggct tcgccaattg
       61 gccagttcct gccgcgggat gactgaaaat ggctccttaa aaaaatgtga cggcagacgg
      121 aagaatttgg cctgaccgcg caactgtacc tgtgaagatc gaagacgatc taccccggac
      181 ccggaatcca acaacgacga cggagacgcc gcgcgtggag gccgacagca gcagccgtga
      241 ggcggcggtc gatatcgagc tgttgcgaaa gtccgccgcg ggtgacggca aggcgttcca
      301 tgcgctggtc gatcggcatg ccgaccggct ttatcggttg gcggtgtcgt tgatcggcaa
      361 tgcggccgac gccgaggacg tgctgcagga agcactcacc ggcgcgtatc gcggcttgag
      421 cggttttcaa gggcgggcat cggtcaagac ctggcttacg cgcatcctgg tgatgcaggt
      481 cgccaagtgg cggcgggatc gtaagcgtcg acgcagcggc gcgaccgcga tcgacgacgg
      541 tgaagcggac gcgcagccgg cggcatcagg cggggcaaca gggggggcgg gcgttgtgga
      601 cgcccggctg gacgtgcacg cggcggtgca gcggttgtcg gcggagcatc gccaagtcat
      661 cgcgctccgc gagttcgagc atctgtcgta tgacgagatg gccgaggtgc tggatctgcc
      721 gcgcggaacg atcgaatcgc ggctgcatcg ggcccgggcg gaactgaaag agattttgaa
      781 atcgtatttg ccgtgacgtc atgaacacct gtgcgctcaa cacgaagctg gatgcgtatc
      841 acgacggcga actcgccggc gcggaactcg catcgttcga gcggcacttg gccgagtgcg
      901 gcgaatgcag ccggcagctg gcacagctgc gggcgatgtc agatctcttt gctgcggcgc
      961 cgcaggtgaa gctgtcgcag atcgcgatgc atcggttgca ccagcggatc gatgtcggcg
     1021 gcgcggacca gggcctgctg cgattgggtt ggacgatgag cgcgatcgcc gccagcttgt
     1081 tgctggtcgg atcggtctgg ctcacgcgga tgagcaaccc gacgaccacg ccggaagccg
     1141 ccccgccgtg ggtgggtgtg ccggtggtgg cgcaggcgga cccggcgtat cgcgagatcg
     1201 cggctacgcc ggcggcgcag tggtatttgg cggatgcgtc aacgcggaac gatgagattc
     1261 cgtgagtcca acgatgaggc gtacgaaatc cgtcatcatc gccgcattca tcctcgtgct
     1321 cagcgcgggc gtggtcgcgg ggcgcttgtg ggcgcggttt ccatcgccca gcgcgacacc
     1381 gacgggaaag cagccctctt ggctggcgga tcagctggac ctgacgccgg aacagcggca
     1441 gcagatggac gccatctggg cgaacgtgaa gcagcagatg gatcagaagc tcgagcgccg
     1501 gcatgcgctg gatcgcgagc gcgaccaggc gatcgaagat ctgctcggcc ccgagcaatg
     1561 gcaggcgtat ggccgcttga tcgatgagtt ccgcgccaag cgcgccgaga tcgacaagga
     1621 ccgcatgaac ctgatccacg atgccaacga tcgcagccgg gcgctgctca gcgacgtgca
     1681 aaagcagcgc tgggaccaga tgcggcaggc gcggcacgat cacggcggcg atggtcacga
     1741 tcatcaccac ggcgacggtc acgagccgac gtcgcaacgc aacaagagcg acgacggcgg
     1801 caacaacaaa acttaattca cctgatcatc catggaacgc gcgagcgcac cgcggggacg
     1861 aatccccacg ggcgacacgc atcaaaattg ttctgcgacg attccccctt taacactccg
     1921 gaaaggagaa tgctcatgca gatgaagacg aagattggct ttctggcggc ggcgttgaca
     1981 ctgggtatgg cggcgttgcc gagcttcgct caacaacagg gcaacggcgg cggcg
//
LOCUS       DATIMH010000841         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1068567, whole genome shotgun sequence.
ACCESSION   DATIMH010000841 DATIMH010000000
VERSION     DATIMH010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068567"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1915)
                     /locus_tag="VLI90_14570"
     CDS             complement(128..1915)
                     /locus_tag="VLI90_14570"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSV15481.1"
                     /translation="MKRPLPILDRLRDSELKSVFFILILVAIALMLALASWAKADITT
                     SIRNTPHNLSASGPGTVKSTNEQQICIFCHTPHNATPIQPLWNRALPASAYRVYASNT
                     LQAKPGQPTGSSKLCLSCHDGTIAVGSVNSRNQQIQMVGGITTLPPGASNLGTDLSDD
                     HPISFRYDASLVSLNPNLRDPGTLPAPVQLDSNKELQCTSCHSAHDDSKGKFLVMDNS
                     NSALCNSCHIPQGTIVQGHDQCADCHQNHTAPSGAHLLRAATVTDTCSLCHGGSAAPL
                     AAAPAARIAARAPDVAAPAAAVQSAPPTVRASSVAQDLQKISRHNADQRAAIVTSAVG
                     SNTKSVAAVNCADCHGSHSMKRDDRGAPNLPGMLGRVSGVTANGGPIATARFEYEVCF
                     KCHGDRSTDRPYINRQLVQPNKRLQFNPSAVSFHPVEARGRNPLVPSLNPNFTPGSII
                     YCMDCHSSDSSRRAGGAGPAGPHGSDNRPLLVARYETTDLTSESASAYALCYRCHQRS
                     SILGNESFPLHASHIVKDRTPCSICHDGHGIASAQGNQMQNAHLINFDVSIVRPDPLT
                     KRLEYRQLGNGRGECFLLCHGKAHSPLSY"
     gene            complement(1912..2952)
                     /locus_tag="VLI90_14575"
     CDS             complement(1912..2952)
                     /locus_tag="VLI90_14575"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HSV15482.1"
                     /translation="MNCLRLSILTCIAALVLASGCAQPAGTLFQPLKEPLRWPAPPEA
                     PRVQYVGQLTNSADLKPAVPFMKSVNRTMFGEEPTQSLLTPYAVCTDNKDRLFIADSN
                     AQLIHVLNLKTRAYTQWKPTGGKQFTQPVGIAYDRPTARLLVADSIGGAIFVFDDHGK
                     FTAEIGRGVLQRPCGLAVDGASMIFVADVAAHQVVMLNPHGDVVKRIGHRGEGLGEFN
                     YPTNVAVDHVGRLYVSDSLNFRVQQFGRDLKPIRQIGRKGDMPGYFAQPKGLAIDRDN
                     HLYVIDSQFEAVQIFDDRGELLLSFGEEGEGPGQFWLPTVISIDDRNRIWIADSYNRR
                     IEVFDYLPEGKS"
     gene            complement(2966..>3548)
                     /locus_tag="VLI90_14580"
     CDS             complement(2966..>3548)
                     /locus_tag="VLI90_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15483.1"
                     /translation="LGVGVRYDIEKSWLKGLSPYARYLIQDQQVDSLRPQDVPIENIR
                     ATVIGADYHIWRLTFNAEHEWHDSSLLPFDAGRYSVRYEDRLSDRVNLSLIGGYTQYV
                     YTDIHNHVNTLNAAAQVDARLSERWTLLGRITYLDDQDDLNGLTRGLEESVELRWKYR
                     QTEIFGRARNANLEGDVTKQDFVTVEMGFRRTF"
BASE COUNT          579 a         1026 c         1221 g          722 t
ORIGIN      
        1 ccacggccgc gccattggcg caggtgataa ctcccctgct tctcggccag atccttcgac
       61 tccggcgaat acgcctaccc tcagaatgac agcggcgccg cgccgctacg accacacgaa
      121 aggcccacta gtaactcaac ggtgagtgcg ccttgccgtg gcagagcagg aagcattcgc
      181 cgcggccgtt gccgagttgg cggtattcca gccgcttggt cagcgggtcg gggcggacga
      241 tgctcacgtc gaagttaatc aggtgcgcgt tctgcatctg gttgccctgc gccgacgcga
      301 tgccgtgacc atcgtggcag atgctgcatg gcgtgcggtc cttcacgata tggctggcgt
      361 gcagcgggaa tgattcgttc ccgaggatgc tgctgcgctg gtggcagcga tagcacaacg
      421 cgtaggcgct ggcgctttcg ctggtgagat ccgtcgtctc gtatcgcgcg acgagcagcg
      481 gacgattatc tgaaccgtgt ggcccggcgg ggccggcgcc accggcccgg cgactgctgt
      541 cggagctgtg gcaatccatg cagtaaatga tgctgccggg cgtgaagttt gggtttaggc
      601 tcggcaccag cgggttgcgc ccgcgcgctt cgacgggatg aaacgagacg gcgctggggt
      661 tgaactgcag tcgcttgttt ggttgcacta actggcggtt gatgtaaggg cgatcggtgc
      721 tgcggtcgcc gtggcatttg aagcagactt catattcgaa gcgcgcggtg gcgatgggac
      781 caccattggc ggtgacgccg ctgacgcgac cgagcatgcc ggggagattg ggggcgccgc
      841 ggtcgtcgcg cttcatcgag tgcgatccat ggcaatcggc gcagttgacg gccgcgacgc
      901 ttttcgtgtt gctgccgacg gcgctggtga cgatggcagc gcgttgatcc gcgttatgcc
      961 ggctgatctt ctgcagatcc tgcgcgacgc tggaggcgcg aaccgtcggc ggcgccgact
     1021 gcaccgccgc ggccggcgcg gcgacatcgg gcgctcgagc ggcaatgcgc gccgcggggg
     1081 cggcagccag cggggcggcg ctgccgccgt ggcagagact gcaggtgtcg gtgacggtgg
     1141 ccgcgcgcag caggtgtgcg ccgctgggag cggtatgatt ctggtggcag tcagcgcatt
     1201 gatcgtgccc ctgcactatg gttccctgcg ggatgtggca ggaattgcat agcgccgaat
     1261 tgctgttgtc catcacgagg aacttacctt tgctgtcgtc atgcgcgctg tggcagctgg
     1321 tgcattgaag ttccttattg ctgtccaatt gcaccggcgc cggcagtgtg ccgggatcgc
     1381 gcaggttcgg gttgagcgag acgaggctgg cgtcgtagcg gaaggagatg gggtgatcat
     1441 cggacagatc ggttcccagg ttgctcgcgc ctggcggcag cgtggtaatg ccgccgacca
     1501 tctggatctg ctggttgcgc gagttgacgc tgccgacggc aatcgtgccg tcgtggcagg
     1561 aaaggcagag cttggacgaa ccggtgggct ggccgggctt ggcctgcaac gtgttgctgg
     1621 catagacgcg ataggcgctg gccggcagcg cgcggttcca cagcggttgg atcggcgtcg
     1681 cgttgtgcgg cgtgtggcag aagatgcaga tctgctgctc gttcgtggac ttcaccgtgc
     1741 ccggtccgct ggcggacagg ttgtgcggcg tgttccggat gctggtcgtg atgtcggctt
     1801 tggcccagga tgccagcgcg agcatcagcg cgatcgcgac caggatcaag ataaagaaca
     1861 cgctcttgag ctcactgtcg cgcagtcggt cgaggatggg gagcgggcgt ttcatgactt
     1921 gccctccggc agatagtcga acacctcaat gcgtcggtta taggagtcgg cgatccagat
     1981 gcggttgcga tcgtcgatgc tgatgaccgt cggcagccaa aactggcccg ggccttcgcc
     2041 ctcttcgccg aaactcagca gcagctcgcc gcgatcgtcg aagatctgaa cggcttcgaa
     2101 ctgcgaatcg atcacgtaca ggtgattgtc gcggtcgatc gccaagccct tgggctgcgc
     2161 gaagtaaccc ggcatgtcgc ccttgcggcc gatctggcgg atcggtttca ggtcgcgccc
     2221 gaactgctgg acgcggaagt tcagcgagtc gctgacgtac aaccggccga cgtgatcgac
     2281 ggcgacgttg gttggatagt tgaattcgcc cagtccttca ccgcggtgac cgatgcgttt
     2341 gaccacgtcg ccatgcgggt tgagcatcac gacctgatgg gcggcgacat cggcgacgaa
     2401 gatcatgctc gcgccgtcca ccgccagccc gcacggccgc tgcagcacac cgcggccgat
     2461 ttcagccgtg aatttcccat gatcgtcgaa gacgaagatg gcgccaccga tggagtcagc
     2521 caccaataag cgggcggtgg ggcggtcgta ggcgatgccg accggctgcg tgaattgctt
     2581 cccgccggtc ggtttccatt gcgtgtatgc gcgcgttttc aggttcagca cgtggatcag
     2641 ctgagcattg ctgtcggcga tgaagaggcg gtccttgttg tcggtgcaga cggcgtaggg
     2701 cgtcagcagc gactgcgtcg gctcttcgcc gaacatcgtg cggttgacgc tcttcatgaa
     2761 cggcacggcc ggctttaggt cggcgctgtt ggtcaactgg ccgacgtact gaactcgcgg
     2821 cgcttccggt ggggcgggcc aacgcagcgg ttccttcagc ggttggaaga gcgtccccgc
     2881 cggctgcgcg cagccgctcg ccagcaccag tgccgcgatg cacgtgagaa tggataggcg
     2941 taggcagttc atattccgct cgctttcaga acgtccgtcg aaatcccatt tcgacggtca
     3001 caaaatcctg tttcgtcaca tcgccttcga ggtttgcgtt gcgggcgcgt ccgaagattt
     3061 ccgtctggcg atatttccaa cgcagctcca ccgattcttc caagccgcgc gtgaggccgt
     3121 ttaagtcgtc ttggtcgtcg agataggtga tgcgtccgag cagcgtccat cgctcggaga
     3181 gccgggcgtc cacttgtgcg gccgcgttca gcgtgttcac gtgattgtgg atatcggtat
     3241 agacatactg cgtgtaaccg ccgatcaggg agaggttgac cctatcgctc agccggtcct
     3301 cgtacctgac ggagtatcgc cctgcgtcga acggcagcag gctgctgtcg tgccactcgt
     3361 gctcggcgtt gaacgtgagt cgccagatgt ggtagtcggc gccaatcacc gtggcgcgga
     3421 tattttcgat cggcacatcc tgtgggcgga gggaatccac ctgttggtcc tggattaaat
     3481 agcgcgcgta tgggctgagg cctttgagcc agctcttttc gatgtcgtac cgcacgccga
     3541 cgcccaga
//
LOCUS       DATIMH010000842         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_199886, whole genome shotgun sequence.
ACCESSION   DATIMH010000842 DATIMH010000000
VERSION     DATIMH010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199886"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VLI90_14585"
     CDS             complement(<1..805)
                     /locus_tag="VLI90_14585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSV15484.1"
                     /translation="MSRNAPVAYSVLDLAPIVQGSNAAGAFGNMLDLAQHGERFGYTR
                     YWLAEHHNIPGVASAATSVVIAHVAAGTRSIRVGSGGIMLPNHAPLMIAEQFGTLESL
                     YPGRIDLGVGRAPGGDTRTARALRRGLPGADTFPQDLIELMNYLGASDPNAAVRAVPG
                     EGLNVPIYLLGSSDFSARLAAELGLPFAFASHFAPDYLHIALQLYRENFKPSEQLSEP
                     YVMVGVNVVAAETDEEGRRLFTSLQQSFLNLIKGVPGEMPPPVEGMDGRW"
     gene            1106..>2627
                     /locus_tag="VLI90_14590"
     CDS             1106..>2627
                     /locus_tag="VLI90_14590"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSV15485.1"
                     /translation="MPTQKHVVPTEVASLPEGQMKEVTLGETKVLLARVNGTLHAVAA
                     ACPHYGMPLAEGLLCGSMLRCAWHQSTFDITTGKLLEPPALDGLATYPISVEDGQVIV
                     TIPEAPTTPPAAPQAASDTRTFVILGAGAAGTAAAQALRDLGFGGRVVLVTAERDQPY
                     DRPNLSKEFLAGQIPADYLPLRPELFYTNANITLLHQRADAIDPAGRTIHFADGSTVT
                     YDKLLLATGAEPRQLTVPGAELANVQTLRSRADCDRLIAAATSCGVHNVVVIGASFIG
                     MEVAAALRERKLDVTVVTPDAVPFQRVLGPEVGGFFQRLHERHGVHFRLNARVDRLDG
                     DHTVRQVTLAAGEQLPAQLVVVGIGVRPATDLLPAQWRDDRGAIPVDEFLRVRQAQHV
                     FAAGDIARYPDPRGDGSLRIEHWRVAEQQGRLAARNMLRDAAEPFVAVPYFWSFQFGV
                     GLDYVGHAGRWDRVQIDGNLAAADFTAYYVRDNRVIAASSCGRSRQMSALLEIMKRD"
BASE COUNT          465 a          963 c          780 g          419 t
ORIGIN      
        1 accaccttcc gtccatcccc tccaccggcg gcggcatctc ccccggcacc cccttgatca
       61 agttcaagaa cgactgctgc aacgacgtga acaaccgccg cccctcctcg tccgtctccg
      121 ccgcgacgac gttcacgccc accatcacgt acggctcgct caactgctcc gacggtttga
      181 aattctcccg atacagctgc aatgcgatgt gcagataatc cggcgcgaaa tgcgaagcga
      241 acgcgaacgg caaccccagc tccgcagcca gacgagcgct gaagtcgctc gaccccagca
      301 aataaatcgg cacgttcaaa ccttcgcccg gcaccgcgcg caccgccgcg tttggatcac
      361 tcgcccccag gtaattcatc agctcgatca ggtcctgcgg aaacgtatcc gcccccggca
      421 atccccgccg aagcgcccga gcggtccgcg tatcaccccc cggcgcgcgg ccaacgccca
      481 gatcgatccg ccccggatac agcgactcca gcgtgccgaa ctgctcggcg atcatcagcg
      541 gcgcatggtt cggcagcata atcccacccg accccacgcg gatgctgcgc gtccctgcgg
      601 ccacgtgcgc gatcaccacc gacgtcgccg cgctggcgac accgggaatg ttgtggtgtt
      661 ccgccagcca atagcgcgtg tagccaaatc gctccccatg ctgagccagg tccagcatgt
      721 tgccaaacgc cccggcagcg tttgagcctt ggacgatcgg ggcgagatcc agaactgaat
      781 aagcgacggg cgcgttgcga gacatcgtgc ccaatcgtag gcgcggccta ctacatgatg
      841 cacccgccgc aggcgtaact gaactttgtg gtccggccct tctctgctcc cctcgcccgg
      901 tactccggga gagggttagg gtgagggtga tttcgttttt tgcgtggccg ctcaaaattc
      961 caaatcaccc tcaccccgac cctctcccgg agtaccgagc gagggggacg gaccagaact
     1021 gagccacaaa ttaaagtcat accccccgcc gcaaaaactg cagaggcctt gggcggcgaa
     1081 accgcgggcg ctatagtctg aacgtatgcc cactcaaaaa cacgtcgtgc ccaccgaggt
     1141 ggcgtcgctg cccgaggggc aaatgaaaga agtcacgctt ggcgaaacca aggtgctgct
     1201 cgcccgcgtc aacggcacgc tgcacgctgt cgccgccgct tgcccgcact acggcatgcc
     1261 gctggccgaa ggcctgcttt gcggctcgat gctgcgctgc gcctggcatc agtccacgtt
     1321 cgacatcacg accggaaaac tgctcgagcc gccggcgctc gatggcttgg caacctatcc
     1381 gatcagcgtc gaagacgggc aggtgatcgt gacgattccg gaggcgccga caacgccgcc
     1441 ggccgcacca caagcggcga gcgacacgcg caccttcgtc atcctcggcg ccggcgccgc
     1501 cggcaccgcc gcggcgcaag cgctgcgcga cctcggcttc ggcggccgcg tcgtcctcgt
     1561 caccgccgag cgcgatcaac cgtacgaccg gcccaacctg agcaaggaat tcctcgccgg
     1621 ccaaatcccc gccgactacc tgccccttcg gccggagttg ttctacacca atgcgaatat
     1681 cacgctgctg caccaacgcg ctgatgcgat tgacccggcg ggtcggacta tccacttcgc
     1741 tgatggatcg accgtcacct acgataagct gctgctcgcc accggcgccg agccgcggca
     1801 actcaccgtg ccgggcgcag agctggccaa cgtgcagacg ctgcgatcgc gcgccgactg
     1861 cgatcgcctg atcgccgccg ccacgtcgtg cggcgtgcac aacgtcgtcg tgatcggcgc
     1921 cagcttcatc ggcatggagg tggccgcggc gctgcgcgag cgcaagctcg acgtcacggt
     1981 cgtcacgccc gacgcggtgc cgttccagcg cgtgctcggc cccgaagtcg gcgggttctt
     2041 tcaacgcctg cacgagcgcc atggcgtgca cttccgcctc aacgcccgcg tcgatcgcct
     2101 cgacggcgac cacaccgtcc gccaggtcac cctcgccgcc ggcgagcagt tgccggccca
     2161 gctggtcgtc gtcggcatcg gtgtccgacc ggcgacggac ctgctgcccg cgcagtggcg
     2221 tgacgatcgc ggtgcgattc cagtcgatga attccttcgc gtccgccagg cgcaacacgt
     2281 cttcgccgcc ggcgacatcg cgcgataccc cgatccgcgc ggcgatggct cgctgcgcat
     2341 cgaacactgg cgcgtcgccg agcagcaagg tcggctggcg gcgcgaaaca tgctgcgcga
     2401 cgccgccgag ccgttcgtcg cggtgccgta cttctggtct tttcagtttg gcgtgggtct
     2461 ggattacgtc ggccacgccg gccggtggga tcgtgtgcag atcgacggca atctcgccgc
     2521 cgccgacttc accgcgtatt acgtgcgcga caaccgcgtc atcgccgcca gcagttgcgg
     2581 ccgcagccgc cagatgagcg cgctgctgga gatcatgaag cgcgatg
//
LOCUS       DATIMH010000843         5938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3089531, whole genome shotgun sequence.
ACCESSION   DATIMH010000843 DATIMH010000000
VERSION     DATIMH010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3089531"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VLI90_14595"
     CDS             <1..340
                     /locus_tag="VLI90_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15486.1"
                     /translation="SYHMGPGGIEAGRFLPSESAPSSVTVSLAPFERQMKRRMLDCFE
                     SQRQTLRYFPLSVECFRIAPRYDFSRPPHEGILFYEKFDWGMTARRFVALAEQAERTL
                     DLVGPVVETK"
     gene            370..1464
                     /locus_tag="VLI90_14600"
     CDS             370..1464
                     /locus_tag="VLI90_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV15487.1"
                     /translation="MRFTVISVAYPFAPVGIDSIGGAEQILSLLDRELVRRGHRSIVV
                     ACEGSKTAGMLLATPAADGVLTEEERSKIQRQHRRTLERALELHPDADLVHMHGIDFD
                     KYLPPPGVPVLVTLHLPASWYSPQVFEIARPQTHLHCVSASQQRACPAGTPLLDDIPN
                     GVPVDELSFRCGKRRYVIALGRICPEKNFHVAMDAATRAGFPLILGGEVFPYPSHLEY
                     FRRQIVPRLTSGHRFLGPLGFARKRRLLSGARCLLNPSLAPETSSLVAMESLACGTPV
                     VAFPSGALPDIIDHGVTGFVVRHEQEMADAVDAAGALDPESCRATARRRFSLEAMVQR
                     YLDSYQQMITASPPPPPPDFYHGGQHATLC"
     gene            1448..3127
                     /locus_tag="VLI90_14605"
     CDS             1448..3127
                     /locus_tag="VLI90_14605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV15488.1"
                     /translation="MPRFARQSHVIVEPITCAASLEAIEPQWWELWQNDPHATPFQSP
                     AWLIPWWRHIGQGELLTIAVRTAEVIAGRNQLVGLFPLYVYTQPESGSRDLFPIGVAT
                     TDYLDPILAQGWEQAASRAAFEWIATQTDRWDLLDFRQLRSSSTCLATPVPEALVEER
                     DTDETCVALTLPRTLAEFQAAAPSHVLHNLEYYRRRACKAGHLNIEPVSQATLDAAFD
                     ALVQLHSARWAARGEGGCLADPTVQMQLRSSMPELLWRQMLRSYVLRLDDRIIGVLYG
                     LSDAPGRAARRFYYYLGGFDPEFERLSPGTLLIGHAIEESIRDGISSFDFLRGQEPYK
                     YLWGARDERTYRRKLRPVPSRNPLPARNPTLCAPARPFDLRLRGVIRPCREDDLPTLE
                     WFGLFLPQREVIREQFERQQRGQAIILIAEVNGIASGQLWIDLQGAPPSTGVFWALRV
                     FPSLQGKGLGSRLITAGEAALSKRNVPFAEISVDQHNAPARQLYERLGYRVAYVTEAG
                     QSVLRKRLSLNISSAHHADHPGQEIHDVKRDPQKREDRRAVDAGRCQQPRV"
     gene            3051..3947
                     /locus_tag="VLI90_14610"
     CDS             3051..3947
                     /locus_tag="VLI90_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15489.1"
                     /translation="MSNATRRKEKIGVRWTLGDVSSRGFEALRLSVWGAWKLFGDEAR
                     YGICVNSISLAHAQRETGSLPAQVLWRAVDRRDLPPVLDEVLDPGMAEGAGWKLAPTR
                     LFPAAFELALDNDCILWRLPPSVRQWITNAERDRCVMAEDVNGCFGQVTDLCGGDPRN
                     AGIRGFPPGFDLSAAVRLVIERCRSRRGRAVRLASETDEQGLQTASLSIHSPPATVTL
                     AEVSICSPFHPHLPNLGSCGAHFVGLNAHHIPWNYYDRPADEWIKQHWHRHLPELYIR
                     TGADRRAPVVETFDSVYQSLEG"
     gene            3950..5089
                     /locus_tag="VLI90_14615"
     CDS             3950..5089
                     /locus_tag="VLI90_14615"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HSV15490.1"
                     /translation="MRAALIVLDSVGIGQAPDAKDYGDEGADTLGHLIGRDASLPRRL
                     PTLWSLGLGNIIGINPVRAPGASYGRMTEVSTGKDSTTGHWEIAGAVITEPFGLFDRF
                     PAELVDAIEREAGVKFIGNCAASGTAIIDQLGPEHLRSGNLILYTSADSVLQIAAHEQ
                     IVPLARLYEVCRIARRHADRYRIGRVIARPFVGSPGTFQRTAGRHDFSMDPPRTVLDC
                     LRESGQPVTAIGKIGDLFAGRAIDQVYPTRSNAEGMHHTEQLWSQMTGGLLFVNLVDF
                     DMLFGHRRDADGYAHALISFDGWLKDFVPMCREQDLLIITADHGNDPTYFGTDHTREQ
                     VPLIVCHKRCAENLGSRESFADVAASLCDFFNLPSSSTGASFLHS"
     gene            complement(5193..5429)
                     /locus_tag="VLI90_14620"
     CDS             complement(5193..5429)
                     /locus_tag="VLI90_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15491.1"
                     /translation="MSVQNAPAYVTGQAEMWGLDMDTFRRFLPEPWTLIAAGAGAGLA
                     ECRCRLQRCIERAGQRSTAPVISRSSRHGFDGRK"
     gene            5557..>5938
                     /locus_tag="VLI90_14625"
     CDS             5557..>5938
                     /locus_tag="VLI90_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15492.1"
                     /translation="MRDRVHFKRRSFYSAFGFAAAGIALPAGAANLLVNPGFEAPQPA
                     AVDQNCTGWTFDNNCQRAQFYNNTPGGNTSIWAKTFQPAGGGIEQVVNVIAGSTYNFQ
                     SYVLFESAYNTTTATIQLGLTFLDA"
BASE COUNT         1069 a         1892 c         1832 g         1145 t
ORIGIN      
        1 ctcttatcac atggggcctg gcggaatcga agccgggcgt tttcttccgt cggaatccgc
       61 cccgtcttcg gtgaccgttt cgctcgcgcc cttcgagcga caaatgaagc ggcgaatgct
      121 cgattgcttc gagtctcagc gccagaccct cagatatttc ccgctcagcg tggaatgttt
      181 tcgcatcgcg ccgcgatacg acttctcgcg gccgccgcac gagggaatct tgttttacga
      241 aaagttcgac tggggtatga ccgctcggcg gttcgtcgcg ctggcagagc aggcggagcg
      301 gacgctggat ctcgtgggtc ccgttgtaga gacaaagtag agacaaaaaa acgttccggt
      361 aagacagccg tgcgattcac cgtcatcagc gtcgcttatc cctttgcccc ggtcggcatc
      421 gactcgatcg gtggtgcgga gcagattctc agcctgctgg atcgcgagtt ggtgcgccgc
      481 ggacatcgtt ccatcgtggt cgcgtgcgaa ggatcgaaaa ccgccggaat gctgctggcc
      541 acccccgccg ccgatggcgt gctcacggag gaggagcgct cgaagatcca gcggcaacac
      601 cggcgcacgc tcgagcgagc gctggaactg cacccggatg cggacctcgt tcacatgcac
      661 gggatcgatt ttgacaagta cctcccgccg cccggcgtgc cggtgctcgt cacgctgcat
      721 ttgccggcca gctggtattc gccccaagtc tttgaaatcg cgcgcccgca aacgcatctg
      781 cactgcgtgt cggcctcgca gcagcgggca tgccctgcgg ggacgccact tttggacgac
      841 atccccaacg gcgtgccggt ggacgaactt tcatttcggt gtgggaagcg ccggtacgtc
      901 atcgcgctgg gacggatttg tccggagaag aattttcacg tcgcgatgga tgccgccacg
      961 cgcgccggtt tcccgttgat ccttggcggt gaagtctttc cgtatccatc gcaccttgag
     1021 tactttcgcc ggcagattgt gccgcggctg acgtcggggc accgcttcct cggcccgctc
     1081 ggattcgcca gaaagcggcg gttgctgtcg ggcgcgcgtt gcctgctcaa cccgtcactg
     1141 gccccggaga ccagctcact tgtcgcaatg gaatcgctcg cctgcggaac gcccgtggtc
     1201 gcgttcccat ccggggcgct gcccgacatc atcgatcatg gcgtcaccgg atttgtcgtc
     1261 cgccacgagc aggagatggc cgacgcggtg gatgccgcgg gggcgctcga tcccgaaagt
     1321 tgtcgcgcca ccgcgaggcg gcgtttctcg ctggaggcca tggtgcagcg gtacctcgat
     1381 tcttatcagc agatgataac ggcctcgccg ccaccgccgc cgccggattt ttatcacggg
     1441 gggcagcatg ccacgctttg ctagacaatc gcacgtgatc gtcgagccga tcacgtgcgc
     1501 ggccagcctc gaagccatcg aaccacagtg gtgggaactg tggcagaacg atcctcatgc
     1561 aacgccgttc caatcgcccg catggttgat tccttggtgg cggcacatcg gacaggggga
     1621 actgcttacg atcgccgtgc gcaccgccga ggtgatcgct ggccgcaacc agcttgttgg
     1681 gcttttccca ctgtacgtgt acacgcagcc cgagagtggt tcgcgcgatc tgtttccgat
     1741 cggagtggcc accacggatt acctcgaccc gatcctcgcc cagggatggg agcaggcggc
     1801 ctcgcgggca gccttcgaat ggatcgcgac gcaaaccgac cggtgggacc ttttggattt
     1861 ccggcagctt cgatcgagct cgacttgtct agccacgccc gtcccggagg cgttggttga
     1921 agaacgcgac accgacgaga catgcgtggc gttgacgctg ccgcggacgc tcgcggaatt
     1981 tcaagccgcc gcgccgtcgc acgtacttca caacctcgag tactaccgca ggcgtgcctg
     2041 caaggcaggc catctaaaca tcgagccggt gtcgcaggcg acgctggacg ctgcgtttga
     2101 tgcgctcgta caactgcaca gcgcacgctg ggcggcgcgc ggtgagggcg gttgcctggc
     2161 ggatcccacg gtgcagatgc agctgcgatc gtcgatgccc gaattgctct ggcggcagat
     2221 gctccggtcg tacgtgctgc gattagacga ccgaatcatt ggtgtgctat acgggctgtc
     2281 cgatgccccg ggcagggccg cgcgccgatt ctactactac ctcggtggct tcgacccgga
     2341 gttcgagcgt ctcagtccag gtacgctgct catcggacac gccatcgaag aatcgattcg
     2401 cgatggcatc tcatcgtttg acttcctgcg cgggcaagag ccgtacaagt acctgtgggg
     2461 cgcccgcgac gaacgaacct accggcgaaa gttgcggccc gttccaagcc gcaatcctct
     2521 tccagcgcgc aatccaacgc tgtgcgcgcc ggcgagacca ttcgacctga ggttacgcgg
     2581 cgtcatccgc ccctgtcgtg aggacgacct gccaacgctc gaatggtttg ggctcttttt
     2641 gccgcagcgc gaggtgattc gcgagcagtt cgagcgacag cagcgcggcc aggcgatcat
     2701 tctcatcgca gaggtaaacg gcatcgcttc aggccaactg tggatcgatc tgcaaggagc
     2761 gccgccatcg accggagttt tttgggcgct gcgggtcttt ccgtcgctgc agggcaaagg
     2821 cctcgggtcg agattgatca ccgctggcga ggccgcgctg tcaaagcgga atgtgccctt
     2881 cgctgagatc tccgtcgatc agcacaatgc accggcgcgc caactctacg agcggcttgg
     2941 ttaccgcgtc gcgtatgtga cggaggccgg gcagtccgtc ctgcgaaagc gactgtctct
     3001 aaatatctca tcagcacacc acgccgatca ccctggtcag gagattcacg atgtcaaacg
     3061 cgacccgcag aaaagagaag atcggcgtgc ggtggacgct gggcgatgtc agcagccgcg
     3121 ggtttgaagc cttgcgcctg tcggtttggg gagcatggaa gctcttcggc gacgaggctc
     3181 gctacggcat ctgcgtcaac tcgatttcgc tcgcgcacgc gcagcgagag acaggttcgt
     3241 tgcccgctca ggtcctttgg cgcgcggtcg accgacgcga tttgccgccg gttctggacg
     3301 aggttttgga tccaggcatg gccgaaggcg cggggtggaa gctggcgcca acgcggctgt
     3361 ttcccgccgc ctttgaactc gcgctggaca acgactgcat cctctggcga ttgccgccat
     3421 cggttcgaca gtggattaca aacgccgagc gggatcgctg tgtaatggcg gaagacgtca
     3481 acggctgctt cggccaagtc acggatctct gcggcggcga tccacgcaac gccggcattc
     3541 gcggctttcc gccggggttc gatttgagcg ccgccgtgcg gttggtgatc gagcgatgcc
     3601 gtagtcgccg ggggcgagcg gttcgcttgg caagcgagac ggacgagcag ggtctgcaaa
     3661 ccgcgtcgct gtcgatccat tcaccgccgg ccaccgtcac cctcgcggaa gtttcgatct
     3721 gctctccgtt ccaccctcac ctgccgaacc tgggttcgtg cggagcccac ttcgtcgggc
     3781 tcaacgcgca tcacattccg tggaactatt acgatcgccc cgccgacgag tggatcaaac
     3841 aacattggca tcgtcacctg ccggagcttt acatcagaac aggcgctgat cgacgcgcgc
     3901 cagtcgtcga gactttcgat tcggtgtatc aatcgctgga gggctagtca tgcgtgccgc
     3961 cctgatcgtc ctggacagcg tcggcatcgg ccaagcgccc gatgccaagg attacggaga
     4021 cgagggcgct gacacgctcg gtcacttgat cggccgcgac gcatcgctgc cgcggcggct
     4081 gccgacgctg tggtcgctgg gcctgggaaa catcatcggc atcaatccgg tacgcgcgcc
     4141 cggcgccagc tatggtcgga tgaccgaagt ttccaccggc aaggacagta cgaccgggca
     4201 ctgggaaatt gccggtgcgg tgatcaccga acccttcggt ctgttcgacc gattcccagc
     4261 ggaactggtt gacgcgatcg agcgcgaggc gggcgtgaaa ttcatcggca actgcgccgc
     4321 cagcggcacc gcgatcatcg accaactagg ccctgaacac ctgcgcagcg gcaacctgat
     4381 cttgtacacg tcggcggatt cggttctgca aatcgccgcg cacgagcaga tcgttccgct
     4441 cgccaggttg tatgaggtgt gccgaatcgc cagacgtcac gccgaccgat accggattgg
     4501 ccgggtgatc gcccggccgt tcgtcggtag cccgggaact ttccagcgaa ccgcgggacg
     4561 ccatgacttc tccatggatc cgccgcgcac cgtgctcgat tgccttcgtg aatcaggcca
     4621 gcccgtgacg gcgatcggca agatcggcga tctcttcgcc ggacgcgcca tcgaccaagt
     4681 ctatcccacc cgttccaacg ccgaagggat gcaccacacc gagcagttgt ggtcgcagat
     4741 gaccggcggt ttgctgttcg tcaacctcgt agacttcgac atgctgttcg gccaccgtcg
     4801 agacgctgac ggctatgccc acgcgctgat cagcttcgac ggttggttga aagactttgt
     4861 accgatgtgt cgcgagcagg atctgctgat catcaccgcc gaccacggga acgatccgac
     4921 ttactttggc acggaccaca cgcgcgagca ggtgccgctg atcgtctgcc acaaacgttg
     4981 tgcggaaaac ctgggctcgc gggaaagctt tgcagacgtg gccgccagtt tatgcgactt
     5041 tttcaacctc cccagttcgt cgacgggcgc ctcatttcta cattcctgaa catctacaaa
     5101 ggccaaaacc ggcgtcgtcg gcttcggcca gcgaggtctc gcgattggcg cgcggccccc
     5161 ctcgcattga tcttcgggcc catccttctc gatcacttgc ggccatcaaa cccgtgccgt
     5221 gacgaccgcg agatcacagg cgcggttgat cgttggcccg cgcgttcgat acaccgctgg
     5281 agccggcagc ggcactccgc cagtcccgcg cccgcgccgg ccgcgatcag cgtccacggt
     5341 tccgggagga accgccgaaa cgtgtccatg tccagacccc acatctccgc ctgtccggtg
     5401 acatacgccg gcgcgttctg caccgacaat tccgtcgcgg aattcccttg acaaatccca
     5461 ggtcgcagac ggacaatgaa agcggttaca tgggcgggaa tgtggccatt tggatggggg
     5521 tcggcgcgcg tcatttcacg gatcctggag gagcgaatga gagatcgagt tcacttcaag
     5581 cggcggtcgt tctacagcgc atttgggttc gcagccgccg gcatcgcgtt gccagcagga
     5641 gcagcgaatc tgttggtcaa ccctggcttc gaggcgccgc agcccgctgc cgttgaccag
     5701 aattgcaccg gttggacgtt cgacaacaac tgccagcgcg cccagttcta caacaacacc
     5761 ccgggcggta acacgtcgat ctgggccaaa acgttccagc cggccggtgg tggaatcgag
     5821 caggtcgtca acgtcatcgc cggttccaca tacaacttcc agagctacgt ccttttcgaa
     5881 agcgcctata atacaaccac cgccacgata caactcggtc tgaccttcct ggacgcct
//
LOCUS       DATIMH010000844         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3376432, whole genome shotgun sequence.
ACCESSION   DATIMH010000844 DATIMH010000000
VERSION     DATIMH010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3376432"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1063
                     /locus_tag="VLI90_14630"
     CDS             47..1063
                     /locus_tag="VLI90_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15493.1"
                     /translation="MRNTAERDNPGDAHPNRSLRLLAPAKINLHLRVGPRRSDGFHPL
                     LTWMATIGLFDSLSLTLDFTRNRSDGVVMSSDDPSLPCDHRNLVVKAANLFREERPSM
                     PAVSIHLEKRVPAGGGLGGGSSDAASTLLGLNRLWVKTVGEAVASTRAAQRAVEGTEE
                     LRGLCEMASRLGSDVPFFLYGPSSICKGRGEAVRPIGGPERAKWVTLVLPPMAMPTAD
                     VYRKFDAMNLGREQDIEREPGWDEWVKLGSRSLLAQLVNDLEPPAFALRPDLGELRTA
                     IERSLDRPVRMSGSGSSLFTLFDEREEAHAAAEQIAANHGVRAVAVELSVQRNPNDET
                     RIPE"
     gene            1275..1748
                     /locus_tag="VLI90_14635"
     CDS             1275..1748
                     /locus_tag="VLI90_14635"
                     /inference="COORDINATES: protein motif:HMM:NF015959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HSV15494.1"
                     /translation="MQLTEVRINLCGGESNRLKAYCSLTFDDTFVIRDVKLIDGNDGL
                     FLAMPSRKLCDRCRRCGEKNHLRARFCNNCGSHLNENRHLLNHTGVGRPKLHADIAHP
                     INVSCREAIERQVLEAYRKEVELSHQPGYVAQSLDCDDDVDFHHQPMRQVAGPQL"
     gene            complement(1768..3339)
                     /locus_tag="VLI90_14640"
     CDS             complement(1768..3339)
                     /locus_tag="VLI90_14640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HSV15495.1"
                     /translation="MPSSNDQRHVNPWLIAVIVSMATFMEVLDTSIANVSLRYIAGSL
                     AAGIDESTWVLTSYLVSNAIILPVSGWLATRIGRKRFYMLCVALFTVSSLLCGLAPSL
                     SFLIVCRVLQGAGGGGLAPSEQAILTDTFPPAQRGQAFALYGVAVVVAPAVGPSLGGW
                     ITDTYSWRWIFFINVPIGILSLLLIYFVVPGIEPSQQTDARAGKRIDYLGFGLVALGL
                     GCLQVVLDRGQIDDWFASTRITIFVIVSGTALILLVIHELTVDGPVVDLRLLGKPSFF
                     IANLIMFLFGGILFGTTQLIPQLLQQLLGYTATLAGLVLSPGALLVICLLPLVGFLLG
                     RGWQPKYLIMFGFLNTAAALWYMSGFDLQISFATAVWARCFQAAGLAFLFVPINTVAY
                     SGLPPGKSNEASALINVMRNLGGSVGISVAQTMLARRSQFHQSRLVEYVAPMNLNYRA
                     AIRGTAQRFTTDAPAGISRMARSIGSIYAELGRQALMMSYLDVFYVMSWAALCSVVAV
                     WFLKKTKPGEHAQAH"
     gene            complement(3363..4592)
                     /locus_tag="VLI90_14645"
     CDS             complement(3363..4592)
                     /locus_tag="VLI90_14645"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSV15496.1"
                     /translation="MARSDAASGHDRRDRPNEPPEAFGRGEQVERAEKPKKDDREQGG
                     DQPDDRGADTGKKDAGKKEEGKPRRPFYKRPVLMAVIIGVALLLGVGGLIWWLHARHF
                     ETTDDAFIDGHIIHVSPRVAGRVISLNVNDNELVPADKVVINIDPAPFEAALNQAKAA
                     QQQAEAKLEQAKADQIVAQANSEQARADVLVAEANATNAARDLTRYQNLMPAARSQQQ
                     LDAATAAQQSTAANLVAAKKRVASVEAQVQSAATNVEAARAAVSAAVAQVQQSSLDLQ
                     YTQVTAGQRGFVTRRTVEAGNFVQVGQDLMTIVQPDVWVTANYKETQLRRMRRGQEVT
                     IHVDAYPDRKLKGHVDSIQTGTGAVFSLLPPENATGNFVKIVQRIPVKIVFDDDPNGR
                     DYVLSPGMSVEPRVKVR"
BASE COUNT          923 a         1554 c         1489 g          899 t
ORIGIN      
        1 tgtcaacgct tgacacccgg atcaagagtg ccaagagtgc cgcgtcatgc ggaacaccgc
       61 tgaacgcgac aatccgggcg atgcacatcc caatcggtcg ttgcgcctac tggcgccggc
      121 gaagatcaac ctgcacctcc gcgtcggtcc gcgacgatcc gatggctttc atccgctgct
      181 gacgtggatg gcgaccatcg ggctctttga cagcttgtcg ttgactctcg atttcacacg
      241 aaacaggagt gatggcgtcg tcatgagcag tgatgacccg tcactcccgt gcgaccatcg
      301 caacctcgtc gtgaaggccg ccaacctgtt cagggaagag cgcccgtcga tgccggctgt
      361 ttccattcat ctggaaaagc gcgtaccggc gggcggtgga ctgggcggcg gcagttccga
      421 cgcggccagt actctgctgg gactgaatcg cctgtgggtg aagacagtgg gtgaggcggt
      481 tgcttccacg cgcgcagcgc agcgcgcggt ggaagggacg gaagaattgc gtgggctatg
      541 cgaaatggct tcgcggctcg gcagtgacgt gccattcttt ttgtacggtc ccagcagcat
      601 ttgcaaagga cgcggcgaag cggttcgccc gatcgggggg cccgagcgtg caaagtgggt
      661 gacgctcgtg ctgcctccga tggcgatgcc gaccgccgac gtctaccgca agtttgatgc
      721 gatgaacctc ggtcgcgagc aggacatcga gcgggagccg ggctgggatg agtgggtgaa
      781 gctcggttcc cgttcgctgc ttgcccagct ggtgaacgac ctggaaccac ctgcgttcgc
      841 gctgcgcccg gacttaggcg aattgcggac ggcgatcgaa cgatcgctcg atcgtccggt
      901 gcgcatgagc ggcagcggga gcagcttgtt cacgctgttc gatgagcgcg aagaagcgca
      961 tgccgccgcc gaacaaattg cagcgaacca cggcgtacgc gccgtcgccg tggagctttc
     1021 tgtgcagaga aatccgaatg acgaaacccg aatccccgag tgaatgacga atgccgaagt
     1081 ccgaatgacg aaacaggaga tgcgtgttcg tcgccctccg tcattcgaca tttgtcattc
     1141 gagtttcgtc attcactcgg ggattcgggt ttcgtcactc gagtttcgcc tctcgggttt
     1201 ccgaagaaat tgtccacgtc gaatgcgcct cgtcatgtca gttcctgtcc gtcggtttca
     1261 acaacgggtg ttttatgcaa ctcacggaag tccgcatcaa cctctgtgga ggagaatcca
     1321 accgcctaaa ggcttattgc tcgctcacgt tcgatgacac gtttgtcatc cgcgacgtga
     1381 agctgatcga tggcaacgat ggcctgttcc tggccatgcc gtcgcgcaag ctgtgcgacc
     1441 gctgccggcg ctgcggggag aagaaccacc tgcgcgcccg cttctgcaac aactgcgggt
     1501 cacacctgaa cgagaaccgc cacctgctga accacaccgg cgtcggccgg ccgaagctgc
     1561 acgcggatat cgcgcacccg atcaacgtgt cgtgtcgcga ggcgatcgag cggcaagtgc
     1621 tggaggcgta tcgcaaggaa gtggagctga gccatcaacc gggatatgtc gcgcagtcgt
     1681 tggattgcga tgatgacgtt gattttcatc accagcccat gcgccaggtc gcggggccgc
     1741 agctttgatc cgcggggcgg ttgccgttta atgggcctgg gcatgctcgc cgggcttggt
     1801 tttcttcaga aaccacaccg ccaccaccga gcacagcgcc gcccacgaca tcacgtagaa
     1861 cacgtcaaga tacgacatca tcaaggcctg gcgtcccagc tcggcgtaga tgctgccgat
     1921 cgaccgagcc atgcgcgaga tgcccgccgg cgcgtccgtc gtgaagcgct gcgccgtccc
     1981 gcggatggcg gcacgatagt tgaggttcat cggcgcgacg tattcgacca gccggctctg
     2041 gtggaattgc gatcgccggg caagcatcgt ctgcgccacc gaaatgccga cgctgccgcc
     2101 caggttgcgc atcacgttga tcaatgctga tgcttcgttg ctcttacccg gcggcaggcc
     2161 gctataggcc acggtgttga tcggcacaaa caggaacgcc aaccccgccg cctgaaagca
     2221 gcgcgcccac acggccgtgg cgaagctgat ctgcagatcg aacccgctca tgtaccacag
     2281 cgcggcggcg gtgttgagaa agccgaacat gatcagatac ttcggttgcc agccgcgccc
     2341 caatagaaaa cccaccagcg gcagcaggca gatgaccagc agcgcgccgg gcgagagcac
     2401 cagccccgcc agcgtcgcgg tataacccag aagctgctga agcaactgcg ggatcagctg
     2461 cgtcgtgccg aacaggatcc cgccaaacaa aaacatgatg aggttggcga tgaagaagct
     2521 cggcttgccc agcagcctca gatcgacaac cggaccgtcc acggtcagtt cgtgaatgac
     2581 caggaggatc agcgctgtcc ccgacacgat gacaaagatc gtaatgcgcg tcgatgcgaa
     2641 ccaatcgtcg atctgcccac ggtccagcac gacctgcagg caacccaaac caagcgcaac
     2701 gagtccgaag ccgagatagt cgatgcgctt tcccgcccgc gcgtcggtct gctgcgaggg
     2761 ctcgatgccc ggcacgacaa agtagatcag cagcagcgac aagatgccga tcggcacgtt
     2821 gataaagaaa atccatcgcc agctgtaggt atcggtgatc cacccgccca gcgatggtcc
     2881 caccgccggg gcgacgacca ccgcaacgcc gtacagcgcg aacgcctgcc cgcgctgggc
     2941 cggggggaag gtgtcggtca ggatcgcctg ttcgctgggc gccagtccgc ccccgcccgc
     3001 gccttgcagc acgcggcaga cgatcaggaa cgacagcgac ggcgccagcc cgcacagcag
     3061 cgaactgacc gtgaacaacg cgacgcacag catgtaaaac cgcttgcgcc cgatgcgcgt
     3121 cgccaaccag ccgctgaccg gcaggataat cgcgttggag accaggtagc tggtgagcac
     3181 ccaggtcgat tcatcgatcc ccgccgccag gctgccggca atataacgaa gcgacacgtt
     3241 ggcgatgctc gtatcgagca cctccatgaa cgtcgccatc gacacgatga ccgcgatcag
     3301 ccaggggttg acatgacgtt ggtcattcga ggacggcatg cgcttggcga aacaaacgac
     3361 cgctatctga ccttcacgcg cggttcaacc gacatccccg gcgacagcac gtaatcgcgg
     3421 ccgttggggt cgtcgtcgaa cacgattttc actggtatac gctgcacgat cttgacgaaa
     3481 ttgccggtgg cgttttccgg cggcagcaag ctgaagacgg ccccggtgcc ggtctggatg
     3541 ctgtcgacgt gacccttgag cttgcggtcc ggataggcgt cgacatggat cgtcacctcc
     3601 tgtccgcggc gcatccgcct cagctgtgtt tccttgtagt tggcggtgac ccagacgtca
     3661 ggctgcacga tcgtcatcag gtcctgcccg acctgcacga agttgcccgc ttccaccgtg
     3721 cggcgcgtga cgaacccgcg ctgacccgcg gtaacctgcg tatattggag atcgagcgac
     3781 gactgctgca cctgtgccac cgccgcactg acggcggctc gcgcggcctc gacgttggtc
     3841 gcggctgact gcacctgcgc ttccaccgac gccacgcgct tctttgccgc caccaggttt
     3901 gccgccgtgc tttgctgcgc cgccgtcgcg gcatccagtt gttgctgcga ccgcgccgcc
     3961 ggcatcaggt tctggtaacg ggtcagatcg cgggcggcat tggttgcgtt tgcctcggcc
     4021 accaacacgt cggcgcgcgc ctgttcgctg ttagcctgtg cgacgatctg atcggccttg
     4081 gcctgttcga gcttggcttc cgcctgctgc tgcgccgcct tcgcctggtt caacgccgcc
     4141 tcgaacggcg ccggatcgat gttgatgacc accttgtccg ccggcaccag ttcgttgtcg
     4201 ttcacgttca atgagatcac tcgcccagca acgcgcggcg agacgtgaat gatgtgtccg
     4261 tcgatgaacg cgtcgtccgt cgtctcaaag tgacgggcgt gcaaccacca gatcaagccg
     4321 cccacgccca gcagcagcgc aacaccgatg atcaccgcca tcaagaccgg ccgcttgtag
     4381 aacggccgcc tgggcttgcc ttcttccttt tttccagcgt cttttttccc ggtatctgca
     4441 ccacgatcgt caggttgatc gccaccttgt tcgcgatcgt ccttcttcgg cttctcagcc
     4501 cgctcaacct gctcgccccg cccgaatgcc tcgggcggct cgttcgggcg gtcgcggcgg
     4561 tcatgaccgg aggcggcatc ggatcgagcc ataggccgtc gatcatagcg cccgccgttt
     4621 atttccgcct accgccagaa tcgcggtgag aacgattcat ggtcgaggcg ccgagttcgc
     4681 cgaggctgac gccgaaagag tcacttggca gggcgatcgc atgtccggtc cccctctccc
     4741 acaattacgt gggcgagggg accagaagaa gcgcatgtgc gatcgcccct gcgtcgccgg
     4801 ggggtgtaac tcctgattgt ggcgaaggcc ttttctggtc ccctcgccca tgtactcatg
     4861 ggaga
//
LOCUS       DATIMH010000845         1715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2510147, whole genome shotgun sequence.
ACCESSION   DATIMH010000845 DATIMH010000000
VERSION     DATIMH010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510147"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="VLI90_14650"
     CDS             complement(<1..1536)
                     /locus_tag="VLI90_14650"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HSV15497.1"
                     /translation="MTSRAIHNRTRRDGPAPIETLEPRRLLSAAGVTTVLDNGPASNR
                     IDLAVVGDGFTQTQIPTFAADVQTFIKGFFNEAPLSSYKSFFNVHEVNVVSNVSGVSG
                     DPTAGVSRSTPLGMSFFTEGVERLLGVDTAAARQYAQSAPGDDQTIAIANSSKYGGAG
                     YPSEDVLTFAGGNPSAVEIVKHEFGHSFGDLADEYDYGGPTNWPGAEPVEADISTYTA
                     AEMVAKKVKWWRWIGMEGVGAYEGAEYSQHGVYRPTQDSKMRTLGQPYGPVDTEQLIE
                     KMYDTVSPIDSATAAGTYPSNTTFSLKVVEPVDHLDAVQWYVDGNAIAGATGLSFDPA
                     GHVMTVGSHTLSAKVEDMTTAVRDETFRSQHMTASRQWTISVGAPPADNTPPTAHYSG
                     GSVTAGNAAYRFSVTYSDNVAVKVGTIDSSDVQVVNSAGGYSQIAKLVSVDHTTSGTP
                     RTATYQITAPGGTWDAGDNGDYTIKLRASQVSDVAGHVAAAGTIGFLHVALPNTIVLS
                     GRVF"
BASE COUNT          268 a          567 c          543 g          337 t
ORIGIN      
        1 gaacacccgg cccgacagca cgatcgtgtt cggcagcgcc acgtgcagaa agccaatcgt
       61 tcccgcggcg gcgacgtggc cggccacatc cgagacctgc gacgcccgca gcttgatcgt
      121 gtaatcgccg ttgtcgccgg catcccacgt gccgccgggc gcggtgatct gatacgtcgc
      181 ggttcgcggg gtgccgctcg tcgtgtgatc gacgctgacc aacttcgcga tctggctata
      241 cccgcccgcg ctgtttacca cctgcacatc ggatgaatca atcgtgccca ccttcaccgc
      301 gacgttgtcg ctgtaggtca cgctgaagcg atacgccgcg ttccccgccg tgacgctgcc
      361 gccgctgtaa tgcgccgtcg gcggcgtgtt gtcggccggc ggggcgccga cgctgatcgt
      421 ccactgccgc gacgccgtca tgtgctggct gcggaacgtc tcatcccgca ccgccgtcgt
      481 catatcttct acttttgcgc tgagcgtgtg gctgcccacc gtcatcacgt gaccggcggg
      541 atcgaacgac agccccgtcg cccccgcgat cgcgttgccg tccacatacc actgcaccgc
      601 gtctaagtga tcgaccggtt cgaccacctt caggctgaac gtcgtgttcg acgggtacgt
      661 gcccgcggcc gttgcactgt cgatcgggct gacggtgtca tacatcttct cgatcaactg
      721 ctcggtatcg accgggccgt agggctggcc gagcgtgcgc atcttcgaat cctgcgtcgg
      781 ccgatagacg ccgtgctggc tgtactcggc gccttcgtac gcgcccacgc cctccatgcc
      841 aatccatcgc caccacttca ccttcttcgc gaccatctcc gcggcggtat aagtgctgat
      901 gtccgcttcg accggttcgg cgccgggcca gttggtcggc ccgccgtagt cgtattcgtc
      961 cgccaggtca ccgaaagagt ggccgaactc gtgcttgacg atttcgaccg ccgacggatt
     1021 gccaccggcg aacgtgagca cgtcttcgga cggatatccc gcgccgccat acttgctgga
     1081 gttcgcgatc gcgatcgtct ggtcgtcgcc cggcgcgctc tgcgcgtatt gcctggccgc
     1141 ggccgtgtcc acgccgagca ggcgctcaac gccttcggtg aagaagctca tgcccagcgg
     1201 cgtgcttcgc gaaacgcccg cggtgggatc gccgctgacg ccggaaacgt tcgagacgac
     1261 gttcacctcg tgcacgttga agaatgactt ataagagctg agcggggctt cgttgaaaaa
     1321 gcccttgatg aacgtctgca cgtcggcggc gaaggtggga atctgcgttt gcgtgaagcc
     1381 gtcaccgacg acggctaggt caatgcggtt cgacgcgggg ccgttgtcga ggaccgtggt
     1441 gacgcccgcg gcgctgagca gtcgtcgcgg ttccagcgtc tcgatcggcg caggcccatc
     1501 gcgacgggtc cgattgtgga tcgctctgct ggtcatacaa atcaacggat ggcgtgtgcg
     1561 tgtaccctcg tgagcgaatc acgttaccga tgaagccgtc gcggcgtcaa tgggcgttac
     1621 gggcgctgtg gtgcggtggt tcgaagggat cggccatcct tctcctgatg gggaggaaaa
     1681 ccgaccacgc cgggttcgga gcgtactcgc gacga
//
LOCUS       DATIMH010000846         4066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_781308, whole genome shotgun sequence.
ACCESSION   DATIMH010000846 DATIMH010000000
VERSION     DATIMH010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781308"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VLI90_14655"
     CDS             complement(<1..843)
                     /locus_tag="VLI90_14655"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSV15498.1"
                     /translation="MSSLAGVPKPDVPPPPPTAAERLLLGQQRVLEQIAVGAPLLDVL
                     NAITKLVEAQEPGIWCSILLLDESGQRLRTAAAPSLPQAFTAAIDGLEIGPEQGACGA
                     AAHLNSRVIVEDTFIDPRTAAFIELAQRYSLRSCWSEPIRSREGTVLGTFAIYSSKPG
                     TPNDMHVRFIETSARLAGIALDRNRAERALRASEYRYQTLAELSPVGIYRADANGQTV
                     YVNPRGLELLGQSLEQARGAGWVNALHPDDAQRVRQHWRDRIGSPHPSLSEFRVIKPG
                     EEPTW"
     gene            1006..1863
                     /locus_tag="VLI90_14660"
     CDS             1006..1863
                     /locus_tag="VLI90_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HSV15499.1"
                     /translation="MEPLGPKLRRQRRRLGLTLDELAGRTGISKPYLSLIETGRVPNP
                     PSDEKLRRLEQTLGFPAGELISQAHLQRTPRDVRAVLSNLLKGAAGGANGGGELKTET
                     KPDKPAAEGAAVAAAAVNLDDAYLSGVLHRMVGKSAGNVEQIATNAVPVINKVSAGYP
                     TDFTDLSYPPQVADDYVGCPDVRDKDAFAARVTGDSMRPKYREGDIVIFSPSLSARSG
                     DDCFVRFEDGQTTFKRIFYESGDNGEAVLRLQPRNEKYRPQVVPSEKVAGLYRAVYRY
                     ERVDQDGDG"
     gene            1979..2446
                     /locus_tag="VLI90_14665"
     CDS             1979..2446
                     /locus_tag="VLI90_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15500.1"
                     /translation="MLSTDFILLRRLRLKIDLIPGPPHNDKVLYSQAIQVIARYPAQE
                     VAQSLSKIANVEVVTDGTRDPRAWQWLWRDGDRSIRLGFTILGKTLDATTAWGGSTLD
                     VDGFVGDLLSLWAQFQKSHSDTWLHYDGHVYTSTGFIDKIRQMLENEVITDIV"
     gene            2759..3265
                     /gene="sixA"
                     /locus_tag="VLI90_14670"
     CDS             2759..3265
                     /gene="sixA"
                     /locus_tag="VLI90_14670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HSV15501.1"
                     /translation="MNVNLVRHGRAGSPVEFARSGMGDDLRPLTIEGRKRMRRNAEGL
                     KAIIGKLDVIVTSPLVRTVETAQIVAKAFGKVTTMELSDLAPGGSREKIVRWMKDQSN
                     DTTVAIVGHEPSLSRLASWLVCGGDRQIFELKKGGACAVHVDGKIAEGGGELLWLMTP
                     RQLRRQAH"
     gene            complement(3228..>4066)
                     /locus_tag="VLI90_14675"
     CDS             complement(3228..>4066)
                     /locus_tag="VLI90_14675"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HSV15502.1"
                     /translation="RDFDNETNERRINTYVKETGGDGEFWRWINQASRRHLPADFVWA
                     DSDFAGYRLLIAAHPKMVDAELVERLTQFVRAGGTLMLSAQSGSKDRNCHLVEQPMPG
                     LLRELAGVEIEDWTTLGKKESRDAELTGGGKIALDTFVERVRCTGATAIAHWRTDDML
                     LREAPAITRHTVGRGSVFYVGGYCPTAAVDGLLDHLQESLKLSPIAQADSEVEVIERV
                     SPSARYTIVLNHSPTVRRVRALPAGREMLADRPVTSEGLSLDPYAVAIVSAPAAAVAA
                     GS"
BASE COUNT          726 a         1245 c         1350 g          745 t
ORIGIN      
        1 ccacgtcggt tcttcaccgg gcttgatcac ccggaattcc gacagcgatg gatgtggcga
       61 accgatgcga tcgcgccagt gctgtcgcac gcgctgggcg tcgtcggggt gcaatgcgtt
      121 gacccaacca gcgccgcggg cctgttccag gctttggccc agcagctcca gcccgcgcgg
      181 attgacgtag acggtctgac cgttggcgtc ggcgcgatag atgccgaccg gcgacaactc
      241 cgccagcgtt tgatagcgat attcgctggc gcgcaacgcg cgctcggcgc gattgcggtc
      301 cagcgcgatg cccgccaatc gcgccgatgt ttcgatgaag cgcacgtgca tgtcgttcgg
      361 cgtgccgggc ttgctggagt agatcgcgaa cgtgccgagc accgttccct cgcgcgagcg
      421 gatgggttcg gaccagcacg agcgaagcga atagcgctgg gcgagttcga taaacgcggc
      481 cgtgcgcggg tcgatgaatg tatcttctac gatcactctg ctattgagat gggcggcagc
      541 gccgcaggcg ccttgctcgg ggccgatctc caacccgtca atcgcggcgg tgaacgcctg
      601 cggcaagctg ggggcggcgg cggtgcggag gcgctggccg ctctcgtcga gcagcaggat
      661 cgagcaccag atgccgggtt cctgtgcctc gacgagcttg gtgatggcgt tgagcacgtc
      721 gagcagcggt gcgcccaccg caatctgctc gagcacgcgc tgctggccta gaagcaggcg
      781 ttccgccgca gtgggaggcg gtggtggcac gtcgggtttg ggaactccag caagcgagct
      841 catccgtttc tcaggccgcc ctttgaatgc ttgcatagta ccgattgtcc tgcgggttta
      901 tacctccgca aatcctgcag cgtagaattc tgagaattag gagaatgttt ggttttaagg
      961 ttgagttttg gatcgggcag cgatatgtaa tggttattaa acatcatgga accgctaggt
     1021 ccaaaacttc gccgacaacg ccgccgactc ggtctaacgc tcgacgaact ggccgggcgg
     1081 accggcattt ccaagccgta cctgtcgctg atcgaaaccg ggcgcgtgcc caatccaccg
     1141 agcgatgaaa agcttcggcg gttggagcag acgctaggtt ttccggcggg ggagttgatc
     1201 agccaggcgc acctgcagcg cacgccgcgg gatgtgcggg cggtgctgag caacctgctg
     1261 aaaggcgctg ccgggggcgc caatggcggt ggggaactga agaccgagac caagccggac
     1321 aaacccgccg cggaaggggc ggcggtcgcc gcggcggcgg tgaacctgga tgatgcgtat
     1381 ttgtccggcg tcctgcaccg gatggtgggc aagtccgcgg ggaacgtcga gcagatcgcc
     1441 accaacgcgg tgccggtgat caacaaggtt tcggccgggt atccgacgga tttcacggac
     1501 ctgtcgtatc cgccgcaggt ggcggacgat tatgttggct gccccgacgt gcgcgacaag
     1561 gacgcctttg ccgcccgggt gaccggtgac agcatgcggc caaagtatcg cgagggggac
     1621 atcgtgatct tttcgccgtc gctgtcggcc cgcagcgggg atgattgttt cgtgcggttt
     1681 gaagacggac agacgacgtt caagcggatc ttttacgaga gcggcgacaa cggcgaagcg
     1741 gtgctgcggc tgcagccgcg gaacgagaaa tatcgcccgc aggtggtgcc gtcggaaaag
     1801 gtggcggggc tataccgcgc ggtttatcgg tacgagcgcg tcgatcagga tggcgatggg
     1861 tagcttgccg cgggcgctgt ggcggttaag ccatttgtat tgtaagaatg acgggacgaa
     1921 cggcttaccc gctggcggac ccgcttaagc ggatcgagcc ggtgattccc gggcacctat
     1981 gctctccacc gacttcatcc tgctccgccg gttgcgcctg aagatcgacc tgatccccgg
     2041 cccgccgcac aacgacaagg tgctgtacag ccaggcgatc caggtcatcg cgcgatatcc
     2101 ggctcaggaa gtcgcgcagt cgttgtccaa gatcgccaac gtcgaagtcg tcaccgacgg
     2161 cacccgcgac ccgcgggcgt ggcaatggct ttggcgcgac ggcgatcgct cgatccgcct
     2221 cggcttcacc atcctcggca aaaccttgga cgccaccacc gcctggggcg ggtccacttt
     2281 ggacgtcgat ggcttcgtcg gcgatctact gagcctctgg gcccagttcc agaagtcgca
     2341 ttcggacacc tggttgcatt atgacgggca cgtctacacc tcgaccggct tcatcgacaa
     2401 gatccggcaa atgctcgaga acgaagtcat caccgacatc gtttaagcag gactccattt
     2461 cagaaccctc atcgtgacac gggcctccgg cccgtgcgtg atgcattggg cttcagaaaa
     2521 ctctggtttc tgctactaac aagcaataag cacgggccgg aggcccgtgt cacgttgaag
     2581 gttcgaaacc gcttcttatc gctcgccgac tggagaatcg ttcgcaatcc tgctgattat
     2641 gacgactgtg ccgccccggc ttccttctga agcgccgggg attcctcatt caatcagggg
     2701 gcagtcgcca caatgcagta caatccgcgc gggcagtttt ggggtttgga gttcgccgat
     2761 gaatgtgaat ctcgtccgcc acggccgcgc cggttcgccg gtggagttcg cccgcagcgg
     2821 catgggtgac gacctgcgcc cgctgaccat cgagggccgc aagcgcatgc ggcggaacgc
     2881 cgaggggctc aaggccatca tcggcaagct ggacgtcatc gtgaccagcc cgttggtgcg
     2941 cacggtcgag acggcgcaga tcgtcgccaa ggcgtttgga aaagtgacga cgatggagtt
     3001 gtccgacctc gcgcccggtg gttcccgcga aaagatcgtg cgctggatga aggatcaatc
     3061 gaacgatacg acggtggcga tcgtcggcca cgaaccgtcg ctcagccggc tggccagctg
     3121 gttggtgtgc ggcggtgatc gtcaaatatt cgagctgaaa aagggcggcg cgtgcgccgt
     3181 gcacgtagac ggcaagatcg ccgagggcgg tggcgagttg ctctggctca tgaccccgcg
     3241 gcaactgcgg cggcaggcgc actgacgatc gcgaccgcgt agggatcgag cgacaggcct
     3301 tcggatgtga cagggcggtc cgccagcatc tcgcgaccgg ccggcagcgc gcggacgcgc
     3361 cgcacggtgg gggagtgatt gagcacgatc gtgtatcgcg cgctcggcga gacgcgctcg
     3421 atcacttcga cttccgaatc ggcctgcgcg atcggcgaca gtttcagcga ctcctgcaga
     3481 tgatccagca gcccatcgac ggcggcagtg gggcaatatc caccgacata gaaaacgctc
     3541 ccgcggccga cggtgtggcg cgtgatcgcc ggggcttcgc gcagcagcat gtcgtccgtc
     3601 cgccaatgcg cgatggcggt cgcgccggtg catcgaacgc gttccacgaa cgtgtcgagc
     3661 gcgatttttc cgccgccggt cagctcggcg tcgcgcgatt ccttcttgcc cagcgtcgtc
     3721 cagtcttcga tctcaacgcc ggccagctcg cgcagcagcc cgggcatcgg ctgctcgacc
     3781 aggtgacagt tccggtcctt cgacccgctc tgcgcgctca gcatcagcgt gccgccggcg
     3841 cgtacgaact gcgtcagccg ctcgaccagc tcggcgtcca ccatcttcgg atgcgcggcg
     3901 atcagcaggc gataacccgc aaagtccgag tccgcccaga cgaagtccgc cggcagatgc
     3961 cgccgcgagg cctggttgat ccaccgccag aattccccgt cgccgccggt ctctttcacg
     4021 tacgtgttga tccggcgttc gttggtctcg ttgtcgaagt cgcgca
//
LOCUS       DATIMH010000847         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4826343, whole genome shotgun sequence.
ACCESSION   DATIMH010000847 DATIMH010000000
VERSION     DATIMH010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4826343"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VLI90_14680"
     CDS             <1..750
                     /locus_tag="VLI90_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSV15503.1"
                     /translation="GSLLRIASEVGELDQDTYVRRRHILQRLLGLIGGCWAICSQIDP
                     LHVNGSGWAVPNTITRAGKLSDYQQNVIDQYLTGQLDALDPCTPHLLKRQSPVVTFCR
                     AEVVDRSWFRSDHFERVRRPLGFGESMYGKLTTPDGRCLKISLHREFSDAPFSDRHVQ
                     LLHVFNENLAGLYISPQRAGGPGPVDTLAPRLRPVLQRLLAGDSEKQAAMKLGLSPHT
                     VHEYTKILYRTFAVNSRGELLAQFVCQGQVC"
     gene            complement(781..1956)
                     /locus_tag="VLI90_14685"
     CDS             complement(781..1956)
                     /locus_tag="VLI90_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15504.1"
                     /translation="MNSRGWKCWAALAGAAVAPVVSSFIATPLWAAPTLSFTDEGVAH
                     DLFGDTAVGYTGWLLTVTADPGHTMDAVDLGPNTASKFGIFGSLLQDWVPGKKFTTPT
                     PVLADQNGGAYGIDTHVLGAGLVSLIGTPVEDNNRLVPAGSGLNNDPSDAFGTGSYLR
                     ANITFNAPATGSVPLAYLVLPSGASPVYSMEIHEDGGVFDLQSIIPPPITPAHNIVWR
                     NNYPGGEPSSYGTNYQFTFITHNDNGTYSPGLLNLPPIGGQYPSEFYMVGHGFSASNP
                     QEVWALNLKVNGVDPTAAQALQIVNDINHDNVGVTATLVSGAYAGLFPGYDVLLYSSG
                     NGTDEWLAIDFSRETSVSGVTVANVAVTVPEPTAIAPIVIATALAAVARRDRKSSDA"
     gene            2351..>3181
                     /locus_tag="VLI90_14690"
     CDS             2351..>3181
                     /locus_tag="VLI90_14690"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSV15505.1"
                     /translation="MSLSLCVLASGSSGNCSVIRAPGGVMLIDCGIGPRTAAGRLKGT
                     GVHVNDIAAICLTHLDRDHFNLNWVDTVIRNQVRVFCAADRADDLRRFVDDEDFAQRI
                     EPFNGDVFEPLPGVSVSSQPLAHDQTGSHAFVVEADGARMGYATDLGRVPAELIERFC
                     GVDLLAIESNYDPQMQQTSGRPRFLQQRIMGGRGHLSNDQALAAVRAVLDRCQRHAHR
                     LPRHIVLLHRSRQCNCPDLVRGLFSRDGRIASRLVLSEQFFRTPWLSAEPVRQWMGEQ
                     L"
BASE COUNT          564 a         1011 c          994 g          612 t
ORIGIN      
        1 ggttcacttt tgcgaattgc ctcggaggtc ggcgagctcg atcaggatac gtacgtccgc
       61 cgccggcaca tcttgcaacg actgctcggc ctgatcggcg ggtgctgggc aatctgctcg
      121 cagatcgacc cgcttcacgt caacgggtcc ggttgggcag tgccaaatac gattacgcgt
      181 gccggaaaat tgtcggatta tcagcagaac gtgatcgacc aatacctgac cgggcagctc
      241 gatgcgctgg acccgtgcac gccgcatttg ctgaagcgac agagcccggt ggtcacgttt
      301 tgccgcgcgg aagtggtgga ccggtcgtgg ttccgatcgg atcatttcga gcgcgtacgc
      361 cggccattgg gttttggcga atcgatgtac gggaagttga ccacgccgga tggccgctgc
      421 ctgaagatct cgctgcaccg cgagttcagc gatgcgccct tcagcgaccg gcatgtgcag
      481 ttgctgcacg tgttcaacga aaacctcgcg gggctataca tcagccccca gcgcgccggc
      541 gggcccggtc cggtcgatac gctcgccccg cgattgcgcc ccgtgctgca gcgcctgctc
      601 gccggcgatt cggaaaaaca ggccgcgatg aagctcggcc tcagcccgca cacggttcat
      661 gaatacacga agattctcta ccgcaccttc gccgtgaaca gccgcggcga actgctggcg
      721 cagttcgtct gccaagggca agtttgctga tgagcaggcg tccggccggt cgagaggtgg
      781 ctatgcgtcc gaggactttc gatcacgcct ggccaccgct gccagcgctg ttgcgatgac
      841 gatcggtgcg atggccgtcg gctccggcac ggtgacggcg acgttggcaa cagtcacgcc
      901 cgaaacagac gtctcgcggc tgaagtcgat cgcgagccat tcgtcggtgc cgttcccgct
      961 gctgtacagc agcacgtcgt agccggggaa taggccggca tatgcgccgc tgaccagggt
     1021 cgcggtcacg ccgacgttgt cgtgattgat gtcgttgacg atctgcagcg cctgcgccgc
     1081 cgtgggatcc acgccgttca ccttcaagtt cagcgcccac acttcctgcg gattgctggc
     1141 cgagaacccg tggccgacca tgtaaaattc actgggatac tgcccgccga tgggcggcag
     1201 gttcagcaag cccggcgaat acgtgccgtt gtcgttgtgg gtgatgaatg tgaactggta
     1261 gttcgtgcca tagctgctgg gctcaccacc gggatagttg tttcgccaga cgatgttgtg
     1321 cgcgggcgtg atcggcggcg ggatgatgct ttgtagatcg aatacgcctc cgtcttcgtg
     1381 gatctccatg gaataaaccg gcgaagcccc gctcggcagc acaaggtatg caagcggcac
     1441 actgcccgtt gcgggggcgt tgaacgtgat gttggcccgc aggtagctgc ccgtgccaaa
     1501 ggcgtcggac gggtcgttgt tcaacccaga ccccgccggt accagccggt tgttgtcttc
     1561 caccggggtc ccgatcaagc tcacgagccc cgcccccagc acatgcgtgt cgatgccgta
     1621 cgcgcccccg ttctggtcag ccagcaccgg cgtcggcgtc gtaaacttct ttccgggaac
     1681 ccaatcctgc aacagcgaac cgaaaattcc gaacttgctg gcggtgttcg gccccagatc
     1741 gacggcatcc atggtgtgcc ccggatccgc cgtcaccgtc agcagccacc cggtgtatcc
     1801 caccgcggtg tcgccgaaca gatcgtgcgc gacgccctca tcggtaaagc tcaacgtcgg
     1861 cgccgcccag agcggcgttg caatgaaaga agacaccacc ggcgccaccg ccgccccggc
     1921 cagcgcagcc cagcacttcc accctctcga attcatgtga accccgcgaa gaagacgttg
     1981 aaaatgcgcc cgctacccgg ccgaatcata cccgggcagg cgcgccgatg cagctctata
     2041 ccaaatggtg gtagcggtgc agtttggtgg ggcagacatt cttgtcggcc tcgcggcgca
     2101 gccgcgaatc aattcaaatc agttctgcgg ccggatgcgg tccttcggcc cgaggcagac
     2161 aggaatgccc caccaaaatt caaactgcac cagtaccagc cattcccttt aattgcttgg
     2221 cgccttggcc tcttggcgca gaggcgatca ccaaaaccga ccgccgctgc ggcaacatca
     2281 ttctcgccct tccgcttgcg tctcgggcag gttttgcaat gatgcacctt cttcatttgg
     2341 agcgacacgg atgtcccttt ccctttgcgt tctcgccagc ggatcctcgg gtaactgctc
     2401 ggtaatccgc gcgcccggcg gcgtgatgct gatcgactgc gggatcggcc cgcgcaccgc
     2461 cgcggggcga ctcaaaggca ccggcgttca cgtcaacgac atcgcggcga tctgcctcac
     2521 ccacctggac cgcgaccact tcaacctcaa ctgggttgac accgttatcc gtaaccaagt
     2581 tcgcgtcttt tgtgcggccg atcgggccga cgatctgcgg cgattcgttg acgacgaaga
     2641 cttcgcgcag cgaatcgagc cattcaacgg cgacgtcttc gaaccgctgc cgggcgtgag
     2701 cgtgtcgagc cagccgctgg cgcacgatca gactggatca catgcgtttg ttgtcgaagc
     2761 cgatggcgcg cgcatgggtt acgcgacgga ccttggccgg gtgccggcgg agttgatcga
     2821 gcgattctgc ggcgttgatc tgctggcgat cgaaagcaac tacgacccgc agatgcagca
     2881 gaccagcggc cggccgcggt ttttgcagca gcggatcatg ggcgggcgcg gacatctttc
     2941 gaatgaccag gcgctggcgg cggtgcgggc ggtgctcgat cgctgtcagc gccatgcgca
     3001 ccggctgccg cggcatatcg tgctgctgca ccgcagccgg cagtgtaatt gcccggatct
     3061 ggtcagaggt ttgttctcgc gcgatgggcg aatcgcgtca cggctggtgc tgagcgagca
     3121 attctttcgc acgccctggc tgtccgctga gccggtgcgg cagtggatgg gtgaacagct
     3181 t
//
LOCUS       DATIMH010000848         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4243718, whole genome shotgun sequence.
ACCESSION   DATIMH010000848 DATIMH010000000
VERSION     DATIMH010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4243718"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1728)
                     /gene="araA"
                     /locus_tag="VLI90_14695"
     CDS             complement(214..1728)
                     /gene="araA"
                     /locus_tag="VLI90_14695"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF002795.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HSV15506.1"
                     /translation="MKNQPAVEIWFITGSQHLYGESALKQVAANSQKMVDALNASGRL
                     PLKLVFKPILTTPEQVSQLCTQANGDASCAGLVLWMHTFSPSKMWINGLLSLQKPFLH
                     LHTQYNRDLPWDRIDMDFMNLNQSAHGDREAGFIHTRLRLGRKVVVGHWSDSEVQDRM
                     AAWMRVARAWIDLHGAKFARFADNMRYVAVTEGDKVTAEAKFGYAVNGYGIGDLVPHL
                     DAVSDAAVDGLCGEYSERYSVAAELRKGGARHSSLRDGARIELGLRSFLEEGNFKGFT
                     TNFEVLHGLKQLPGLAVQRLMNDGYGFGAEGDWKTAALLRAMKTMAIGLKGGTSFMED
                     YTYHLHPNGHQVLGSHMLEVCESIAAGKPTLEVHPLGIGGKEDPVRLVFDAPPGPALN
                     ASMVDLGNRFRLIVNEVDAVKPPQPLPKLPVARAVWECRPDFKTACAAWIYAGGAHHT
                     VFSYAVTREHLEDFAEIAGIEMVTIGRGTEIGGFRQQLRNNEVYYHLAGGFSRM"
     gene            2106..>2557
                     /locus_tag="VLI90_14700"
     CDS             2106..>2557
                     /locus_tag="VLI90_14700"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HSV15507.1"
                     /translation="MLRYARKLFARTEAPHVHAGLPLMAAPARAPLSLHDLLGRPPVN
                     LDTPEIQRFLAGKRVLVTGAGGSIGSEICRQAMKFVPQRLLLVERAENALFEIDRELR
                     HRWLGAEIRPYVADICDAQRVAQVFAAERPHVVFHCAAHKHVPMMEINP"
BASE COUNT          444 a          838 c          792 g          483 t
ORIGIN      
        1 acgcccgcgt cgccgcgtgc gtagttgtag atgaacgagc catactcctg cccgttggcg
       61 acattgacga tcagcgcgcg gacggagttg gtgccgaagt cgaggccgat cgtgaagtgg
      121 ttgtccatgg cgcgaagctc ctaatgcaga cgatggatgt gatgctcttt ccccctctcc
      181 cggagtaccg ggcgagggga ccagacgcag tcctcacatc cggctgaatc ccccggccag
      241 atgataatac acttcgttgt tccgcagctg ctggcgaaag ccgccgattt ccgttccgcg
      301 gccgatcgtg accatctcga ttccggcaat ctccgcgaag tcttccaggt gctcgcgcgt
      361 caccgcatag ctgaagaccg tgtgatgcgc gccgccggcg tagatccacg cggcgcacgc
      421 ggtcttgaaa tctggccggc attcccacac cgcgcgggcg actggcagct tcggcagcgg
      481 ctgcggcggt ttcactgcat ctacttcgtt cacaataagc cgaaagcggt tgcccaggtc
      541 caccatcgac gcgttgagcg ccggtcccgg cggcgcgtcg aacaccaggc ggaccggatc
      601 ttccttcccg ccgattccca gcgggtgcac ctccagcgtc ggcttgcccg cggcgatcga
      661 ttcgcagacc tccagcatat gcgaaccgag cacctggtgc ccgttgggat gcaggtgata
      721 cgtgtaatct tccatgaacg acgtgccgcc cttgaggccg atcgccatcg tcttcatcgc
      781 ccgcagcaac gcggcggttt tccagtcgcc ctcggcgccg aagccgtaac cgtcgttcat
      841 caaccgctgc accgccagcc ccggcagctg cttcaggcca tgcaagactt cgaagttcgt
      901 ggtgaagccc ttgaagttgc cctcttcgag aaagctccgc aggcccagtt cgatgcgagc
      961 gccgtcgcga agggatgagt gccgcgcgcc gcccttgcgc agctcggcgg ccacgctgta
     1021 gcgttcagaa tattcgccgc agagaccatc gacggcggcg tcgctcaccg cgtccaagtg
     1081 tgggacgaga tcgccgatgc cgtacccatt gacggcgtat ccaaacttcg cctcggcggt
     1141 gaccttgtcg ccctcggtca cggcgacgta acgcatgttg tcggcaaagc gcgcgaactt
     1201 cgcgccgtgc agatcaatcc aggcgcgggc cacgcgcatc cacgcggcca tgcggtcctg
     1261 cacttcgctg tcggaccaat gtccgacgac gaccttgcgg cccagccgca ggcgcgtgtg
     1321 gatgaacccg gcctcgcggt cgccgtgggc cgactggttc aggttcatga agtccatgtc
     1381 gatgcgatcc cacggcaggt cgcggttgta ttgggtgtgc agatgcagga acggcttctg
     1441 caacgacagc aggccgttaa tccacatctt cgacggcgag aacgtgtgca tccacaacac
     1501 caggcccgcg cagctggcat cgccgttcgc ctgcgtgcag agctggctga cttgctccgg
     1561 cgtggtcagg atcggcttga aaaccagctt gagcggcaat cgcccggatg cgttgagcgc
     1621 atcgaccatc ttctgcgagt tcgccgccac ctgtttcagg gcggattcac cgtacaggtg
     1681 ttggctgccg gtgatgaacc agatttcgac cgcgggttgg tttttcatga gcgcaagctc
     1741 gcagtaaggg accgccgggt gcgctgcgcg tgggtgcgcg cggcgcagta ataaaagttg
     1801 ccgtgtcgag attagaaccg gtgcgcgaag gaataaaacc cgaggcgcgg tggctcgttc
     1861 cctgccaatt tctccagaac gcgcaattat cgcgccgatg ggacgattgt caatcgcggg
     1921 ggatggagtc acgacctctt gtcgcaacaa caagacccgc gcagcgcgct atcagggcgc
     1981 ggttcactgg agcatccttc atccgctcga cattcatggt tctgcattcg cctcgccgcc
     2041 ccacgtccga gaatgtttga accgcgacgc ccggattagc cgatagatgc caagtggatc
     2101 acccgatgct tcggtacgcg cggaaactgt ttgcccgcac cgaagccccg catgtacatg
     2161 cggggctccc gttgatggcc gcgcctgcgc gcgcgccgct ctccctgcac gatttgctcg
     2221 gccgtccgcc cgtcaatctg gatacgccgg aaatccagcg cttcctcgcg ggcaaacgcg
     2281 tgctcgtcac cggcgcgggc ggcagcatcg gttcagagat ctgccggcag gcgatgaagt
     2341 tcgtgccgca gcggctgctg ctggtggagc gcgcggagaa tgcgctgttc gagatcgacc
     2401 gcgagctgcg ccaccgctgg cttggcgccg agattcgccc ctacgttgcc gacatctgcg
     2461 atgcgcagcg cgtcgcccag gtctttgcgg ccgagcgccc gcacgttgtc ttccactgcg
     2521 cggctcacaa gcacgtgccg atgatggaga tcaaccc
//
LOCUS       DATIMH010000849         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5115854, whole genome shotgun sequence.
ACCESSION   DATIMH010000849 DATIMH010000000
VERSION     DATIMH010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5115854"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VLI90_14705"
     CDS             complement(<1..686)
                     /locus_tag="VLI90_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15508.1"
                     /translation="MSRPSHVAADLSVRQVATAWPACAALLNGYAGARVDGRWTLQDL
                     APFASDRGLQQKSFLRELASAAGVTVAQTGRPIERASPVLLIFIATAICLTVGAGWGV
                     ALLLRIAVGVDYRAVSGASVHVHGLAQLWGWMALFVFAVATHLLRQNTKRPAPPWLEY
                     AAAGLIVAALVVFFGGLIDPLRRAIPQIDVIASISLAGAVTFFGISVIWSLTGRAKSQ
                     RSHGFVFLIG"
     gene            complement(699..953)
                     /locus_tag="VLI90_14710"
     CDS             complement(699..953)
                     /locus_tag="VLI90_14710"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HSV15509.1"
                     /translation="MSPKNTIDVRTIPGPQRHPFIFHTFEALAPGEALELVNDHDPFP
                     LRNQFNFMKRGQFSWDYLEEGPELWRVRIAKVAQPQASSK"
     gene            complement(1093..1845)
                     /locus_tag="VLI90_14715"
     CDS             complement(1093..1845)
                     /locus_tag="VLI90_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSV15510.1"
                     /translation="MASTSRHSGETPDQSDQLRLFRNLEPAARDQIVAAGISVRRTRG
                     TRLFRQGAAAQSFYVLRQGRVKLTQLTPDGHLVLLRLVVPGEAFGGIAALGQRTYPVS
                     AEAAEDSVALSWNGRTMDRLLRVHPQLAINFIEFLAERLHDMQCRYEELATEQVEQRL
                     ARMLLRLVQRAGRRVDGGVLVDVRLSREELAEMTGTSLFTVSRILNRWQQAGVIQLRR
                     QRILVRHPHGLVTIAESLPPSDRNREANGAEI"
BASE COUNT          379 a          624 c          586 g          353 t
ORIGIN      
        1 cagccgatga gaaacacgaa tccatgcgaa cgctgtgatt ttgctcgtcc ggtcaatgac
       61 cagatgaccg atatgccgaa gaaggtcacc gcgccggcaa gcgagattga agcgatgacg
      121 tcgatctgtg ggatcgcccg ccgaagcggg tcgatcagcc cgccgaaaaa caccaccagc
      181 gccgccacga tcaatcccgc cgccgcatat tccagccagg gtggcgcggg ccgtttggtg
      241 ttctgtcgca acagatgtgt cgcgacggca aacacaaaca acgccatcca cccccaaagc
      301 tgtgctaagc cgtgaacgtg tacgctggca ccggagaccg ctcgatagtc aacccccacg
      361 gcgatgcgga gcagcagtgc cacgccccac cccgcgccaa cggtcaagca gattgccgtg
      421 gcaatgaaga tgagcagcac cggcgaagcg cgttcgatcg gcctgccggt ttgagcaact
      481 gtcaccccgg cggcgctggc cagctccctt aggaaagact tttgttgcag cccgcgatcg
      541 ctggcaaacg gcgccaagtc ctgcagtgtc catcgcccat cgacgcgagc gccggcataa
      601 ccgttcagca gggccgcgca cgccggccat gccgtagcga cttgccgcac gctcaggtct
      661 gctgcaacat gtgatggccg actcatggat ctgtcggtct atttcgatga agcttgcggc
      721 tgcgcgacct ttgcgatgcg gacgcgccaa agttccgggc cttcttccag ataatcccaa
      781 ctgaactgcc ctcgcttcat gaagttgaat tggtttcgca gcgggaacgg atcgtgatcg
      841 ttgaccaatt ccagcgcctc gccgggcgca agcgcctcga aggtgtggaa gatgaacggg
      901 tgccgctgcg ggccgggaat cgtgcggaca tcaatcgtgt tcttcgggct cactgtattc
      961 gctcctggtt gttgggtcag atagcactcg cgcgggactt atccgcgtcc tcgcgggctt
     1021 tgctcttaca ttagggctca tgcggcgttc gttctttgct ccagagcaaa gagccgaaca
     1081 aatcccgcgg catcaaattt ctgcaccgtt ggcctccctg ttgcgatccg aaggcgggag
     1141 tgattcggcg atcgttacga gcccgtgcgg gtgtcgcaca aggatgcgct ggcgacgcaa
     1201 ctggatcacg ccagcctgct gccagcggtt gaggatacgg ctgaccgtaa acaggctggt
     1261 gccggtcatt tccgccagtt cttcacgcga gagccgcacg tccaccagca ccccgccgtc
     1321 cacccgccga ccggcgcgct gcaccaaccg cagcaacatc cgcgccagtc gctgctcgac
     1381 ctgttcggtg gccagttcct cgtagcggca ctgcatgtca tgcaggcgtt ccgcgagaaa
     1441 ctcgatgaag ttgatcgcca gttgcgggtg tacgcgaaga aggcgatcca tcgtgcggcc
     1501 gttccaactt agcgccactg aatcctcggc ggcttcagcc gaaaccggat aggttcgttg
     1561 tccaagagcg gcgatgccgc cgaacgcttc gcccggcacg accaggcgga gcagaaccag
     1621 gtgaccatcg ggtgtgagtt gtgtgagctt cacccgcccc tgccgtagaa cgtaaaagga
     1681 ctgagctgcc gcgccctgtc gaaagagtcg cgtcccacga gtgcggcgaa cggaaatacc
     1741 tgcggcaacg atctggtcgc gcgcggccgg ctcgaggttg cggaacagcc tgagctgatc
     1801 tgactgatcc ggagtttccc cactatgccg actggtgctc gccacgcgac tatcatatgc
     1861 gccggggcga acgcggcgag acagccgctt cagcccgcat cgcgcagcag cccgcggcgc
     1921 ggcgccatct gcttgtcaat ct
//
LOCUS       DATIMH010000850         9312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2222451, whole genome shotgun sequence.
ACCESSION   DATIMH010000850 DATIMH010000000
VERSION     DATIMH010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222451"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /gene="thrS"
                     /locus_tag="VLI90_14720"
     CDS             complement(<1..1756)
                     /gene="thrS"
                     /locus_tag="VLI90_14720"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HSV15511.1"
                     /translation="MASPGNVKTGDSIAGFTKEADETMAAIKLPDGSVREIPDGSTVQ
                     QLAESIGRGLAKAAVAAKVDGKVVDLSTKLSGLHTVSILTDRDPEALLVLRHSAAHVM
                     AEAIQRLWPKAQLAYGPPLDTGFYYDIALDQPISANDFDRIEKEMREIVKEDRPFTRY
                     EKSFDEGMQRLRSENNKYKIDNAERAKAAGSACLSWYATGAPGSNWEDLCMGPHVPST
                     GKIKAFKVMSVASSHWHGDISSDRFQRVYGTAFFDPKQLEQHLKVLDEAKKRDHRVLG
                     PQLGLFTIDDQVGQGLVLWKPKGAEVRIQLQNFISEHLRRQGYKQVFTPHIGRLDLYK
                     TSGHFPYYRESQFPPLIDREFYEQMAKDNCGCADLSNRMEKGDIDGYLLKPMNCPHHI
                     RIYASDQHSYRDLPIRLAEFGTVYRWEKSGELGGMTRVRGFTQDDAHLFCTEEQVPAE
                     IRGCLELVKIVLGTLGMNDYRVRVGLRDPDSAKYVGDPEQWDKAEQACKDAASSLGVS
                     FTLEPGEAAFYGPKIDFVVKDVIGREWQLGTVQVDYQLPKRFDLSYVGADNAPHRPVM
                     IHRAPFGSMERFIGVLTEH"
     gene            2162..2422
                     /gene="rpmB"
                     /locus_tag="VLI90_14725"
     CDS             2162..2422
                     /gene="rpmB"
                     /locus_tag="VLI90_14725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSV15512.1"
                     /translation="MPRVCYFTGKKTTFGNQITHRGKAKYLGGVGTKVTGITARKFKP
                     NIQKVRAVIDGQIVRVKVSAKAIKQGLIVKPVRRKYTKPAAA"
     gene            2524..2841
                     /gene="gatC"
                     /locus_tag="VLI90_14730"
     CDS             2524..2841
                     /gene="gatC"
                     /locus_tag="VLI90_14730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HSV15513.1"
                     /translation="MPDAKITIDDVRHVAKLARLDLPEDRLNTLTSQLESILEYVAKI
                     GEVDVTSVEPMAHALPLHNVFREDIVEPSLPLEKVLQNAPETDGPFFKVPKVIGGDED
                     SAD"
     gene            2868..4178
                     /locus_tag="VLI90_14735"
     CDS             2868..4178
                     /locus_tag="VLI90_14735"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HSV15514.1"
                     /translation="MPHPTWENLLENALSARDATNERRARRALRVMDATHVEQDGRRL
                     VNFASNNYLGLTHHPRVIDAFDSAAKRDGVGSAAAALVTGYTDAHASAERAIAAWKGS
                     ESAVMLSSGYAANLAAVQAIAAVAGGERRVRFLLDKLAHASLIDAVRGSGAAYRVFPH
                     NHLEKLERLLVEAEPGQLQVLVTESIFSMDGDAADLAGIARLKQKQPFVLLLDEAHGS
                     GVYGVNGAGYASEIGLQSIVDVTIVTLSKAIGVSGGAVCASRAFCDALINFGRSYIYS
                     TNVPPAVAAAAEASIAVMRDEPQRQQRVRALARDFRAGLMEMGLRLAPGGPHAGFGGT
                     PTTRILPRSGDEESGSSRVRSEPGVAGAYSEGSAWDSPILPVILGGESESLKAAERLR
                     EAGFLVVAIRPPTVPRGSSRLRITLSSEHRDEEVRGLLEALQSL"
     gene            complement(4273..4548)
                     /locus_tag="VLI90_14740"
     CDS             complement(4273..4548)
                     /locus_tag="VLI90_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15515.1"
                     /translation="MNRKFSEEDRRAVDLLLDHGAATSGVTRVAHAVSQKRLAAAERV
                     LKLLGQMPAGEPSIDLVAATMQRVDRASAGHVGRATRRTHLPQPPVA"
     gene            complement(4807..5628)
                     /locus_tag="VLI90_14745"
     CDS             complement(4807..5628)
                     /locus_tag="VLI90_14745"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV15516.1"
                     /translation="MKSTRPQSHKARSGHSPSNGKSNGNGHAAGGAKTAAAKPAGTNV
                     HGGNGHGKYMNPDSSPLLATAAQPGMVESDRGQRTDEQLIAAYRSGDRSSFAELVGRY
                     QRELFHFLIRFLGDRAAAEDVFQETFLQIHQSAENFDTQRRFRPWLFTIAANKARDLI
                     RANARRPVSPLQASISPGDDDSSEFIDLMKSATDLPDEPMQQQELQNKVKQTVMNMPE
                     HLREILLLSYFHQFPYKQISDILDIPLGTVKSRLHAAVAHFADRWRSANPSHAMS"
     gene            5713..6816
                     /gene="rlmN"
                     /locus_tag="VLI90_14750"
     CDS             5713..6816
                     /gene="rlmN"
                     /locus_tag="VLI90_14750"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HSV15517.1"
                     /translation="MPSSVQHPADAPKVAFFALTAEQVDRLVQTWGWPRFRGRQVREW
                     VYHKGVVDPEQMTNLGKADRALLTARFDFGSSTIVNHQSSSDGTEKLLLAWPDGNNAE
                     TVMIPDGPRRTACVSSQVGCPAGCKFCASGVNGVKGNLSADQIVEQIVRLNALLKRGD
                     ERITNVVFMGMGEPLANYANVMQAIRVLHDPGCLNIGARKITVSTVGVPPKMRQLAQE
                     ELPLNLAISLHAPNEPLRKELIPWAEHFALDDILNAARYYFQQTGREITLEYILLAGV
                     NDKPEHARQLAKLCKTLRANVNLIRYNEVAGLPFGRPKADDVTVFQDILRNNGVNAHV
                     RKSRGRDIDAACGQLRRKQQEQQEQLITIGTPA"
     gene            6918..7952
                     /locus_tag="VLI90_14755"
     CDS             6918..7952
                     /locus_tag="VLI90_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV15518.1"
                     /translation="MVPSSSRSKFRRGFSAVELLVVIGILLVLVSIFVPYLLKIREMD
                     HRVKCADNLRIIRAGLAQYSTTNNGDYPRVVYDTTHPGYACYTGVDGGSPFAKDSGVQ
                     ANDVTASLWLLVRMKLVEPRAFICPSTSDDIDPIDDASGHAVGVELRGNFRGPDSLSY
                     SYASPFSAAPGYRLNDTQRSGFAVMADKNSGNAASVPFDAQPLQFAAANSANHGQAGQ
                     NVLYADGHVDFQPTPYCGVERDNIFTALALKPLEKGQESPPKFSSGITSRDVSPAWES
                     DSYLVPTASDRVPTTGPTTSTTAASTQAATAPATGPVTVPPPPQAPPPPPPPPPPPTT
                     LPTTGPSTRP"
     gene            8105..9052
                     /locus_tag="VLI90_14760"
     CDS             8105..9052
                     /locus_tag="VLI90_14760"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HSV15519.1"
                     /translation="MHYRRLGEAGVKLSEIGLGGWLTFGNAMEAQQARSVIDKAFDVG
                     INFFDTADAYAGGQAEEAWGELFKSRVRSSYVLATKVFFPTGQGANDRGLSRKHVFEN
                     CHKSLKRLRTDYIDLYQCHRFDPETPLEETVGVMDDLVRQGKILYWGFSEWTAEQVSQ
                     CLQVCGDRYERPRSSQPRYSALERYVEKDVMPLCHKAGIGQVVFSPLAQGVLTGKYKP
                     GQAFPADSRAADDRQNQFIKRFVDDPQLLEKVQRLVPLAEEHHCSMSQLALAWVLRRA
                     EVTACIIGASKPQQIDENAEASGIKLSEEDARRIDEVLS"
BASE COUNT         1740 a         2939 c         2924 g         1709 t
ORIGIN      
        1 agtgttcggt gaggacgccg atgaaacgtt ccatgctgcc gaacggggcg cggtggatca
       61 tcaccggtcg atgcggggcg ttgtcggcgc cgacgtagct caggtcgaac cgcttgggca
      121 actggtaatc cacctgcacc gtgcccagct gccactcgcg gccgatcacg tccttcacca
      181 cgaagtcgat cttcggcccg taaaacgcgg cttcgccggg ttcgagcgtg aacgacacgc
      241 ccaggctcga cgcggcgtct ttgcacgcct gctcggcctt gtcccattgc tcgggatcgc
      301 cgacgtattt cgcgctgtcg ggatcgcgca ggccgacgcg cacgcgatag tcgttcatgc
      361 cgagcgtgcc gagcacgatc ttcacgagtt ccaaacagcc gcggatttcc gcgggcactt
      421 gttcttcggt gcagaacagg tgcgcgtcat cctgggtgaa gccgcgcacg cgggtcatgc
      481 cgccaagctc gccgcttttt tcccagcgat agacggtgcc gaattccgcc aggcggatcg
      541 gcagatcgcg atagctgtgc tgatcgctgg cgtagatgcg aatgtgatgc gggcagttca
      601 tcggcttcag caggtaaccg tcgatgtcgc ccttctccat gcggttgctc aggtccgcgc
      661 agccgcagtt gtccttggcc atctgctcgt aaaattcgcg gtcgatcagc ggcgggaact
      721 ggctctcgcg gtagtacgga aaatgcccgc tggtcttata caaatccagc cggccgatgt
      781 gcggcgtgaa cacctgcttg tatccctggc gccgaaggtg ctcgctgatg aagttctgca
      841 gctggatgcg cacctccgcg cccttcggct tccacagcac gaggccctgg ccgacctgat
      901 catcaatcgt gaataaaccc agctgcgggc cgagcacgcg gtggtcgcgc ttcttcgctt
      961 cgtcgagaac cttcaggtgc tgttcgagtt gcttgggatc gaagaacgcg gtgccgtaga
     1021 cgcgctggaa gcgatcgctg ctgatgtcgc catgccagtg tgagctggcg acgctcatga
     1081 ccttgaacgc cttgattttt ccggtgctgg gcacgtgcgg gcccatgcac aggtcttccc
     1141 aattgctgcc gggcgcgccg gtggcgtacc agctgaggca agccgacccc gcggccttgg
     1201 cgcgctcggc gttgtcgatc ttgtacttgt tgttttcact acgaaggcgc tgcatcccct
     1261 cgtcgaacga cttttcatag cgcgtgaacg ggcggtcctc cttcacgatc tcgcgcatct
     1321 ccttctcgat gcggtcgaag tcgttggcgg agatcggctg atccagcgcg atgtcgtaat
     1381 agaaaccagt atcgagcggt ggaccgtacg ccagctgcgc cttgggccac aggcgctgaa
     1441 tggcctcggc catcacgtgg gcagcgctgt ggcgcagcac gagcagcgct tcgggatcgc
     1501 ggtcggtcag gatcgagacg gtgtgcaagc cgctcagttt cgtcgagagg tcgacgacct
     1561 tgccatccac cttggccgcc acggcggcct tggccagccc ccggccgatg gactcggcca
     1621 gctgctgcac ggtgctgccg tcggggattt cgcgaacgct gccgtcgggt aatttgatcg
     1681 ccgccatggt ctcatccgcc tcttttgtaa agccggcgat gctatcgccg gtcttcacat
     1741 tccccggcga tgccatcgcc ggctttacat gaaaacaaaa cataaaaaca aaatcgcccg
     1801 gtccgtaaga accgggcggt cgtcatcgca ctgaaggctt gctgaagcgc gcgccacgcg
     1861 atcaccaccc ggtgcttcgg gtcgtgggcg tcgtcgcctg gaaaatgcgt gcaacgcaag
     1921 ccatggctga gaaatatatg acacgcgccg cagcgggtca aaccacgccg caggcgcggg
     1981 atgatgtagc cgcgacgcct gcgtcgcctc tgccgcaatt acagagcggg gcgggcgcac
     2041 tgcagcggct tgttcgtcgt tttattgctt cgatcgcagg aagcttgcag gccggcccgc
     2101 tcattcctat actacccggc tcgaattttg gaagcgttat acgactagag gttgacccgc
     2161 aatgccacgc gtttgctatt tcaccggtaa gaagacgacc ttcggcaacc aaatcaccca
     2221 ccgcggcaag gccaagtatc ttggcggcgt cggaaccaag gtgaccggca tcaccgcgcg
     2281 caagttcaag ccgaacatcc agaaggtccg cgccgtcatc gacgggcaga tcgtccgcgt
     2341 gaaggtcagc gccaaggcca tcaagcaggg cctgatcgtg aagccggttc gccggaagta
     2401 caccaagcct gccgcggctt aagccgcgca tggcagatgt gaaacggaaa atggcaaatg
     2461 gaggacgagc caatcggctc cgatccattt gccattttcc atttaacatt taccatttcc
     2521 cccatgcccg atgcgaagat cacgatcgac gacgtccgcc acgtcgccaa gctggcccgg
     2581 ctcgacctgc cggaagaccg gctgaacacg ctgacgagcc aactcgagtc gatcctcgaa
     2641 tacgtcgcca agatcggcga ggtggacgtg acgagcgtcg agccgatggc tcacgccctg
     2701 ccgctgcaca acgtctttcg cgaagacatc gtcgagccca gcctgccgct ggaaaaagtc
     2761 ctgcaaaacg cccccgagac cgacggcccg ttcttcaaag tccccaaggt catcggcggc
     2821 gacgaagact ccgccgactg attcgaacgc acctcttcca aaagcccatg ccccacccca
     2881 catgggagaa cctgctcgaa aacgcgctat ccgcccgtga cgcgacgaat gagcgccgcg
     2941 ctcgccgggc gttgcgcgtg atggacgcga cgcacgtcga gcaggacggc cggcggttgg
     3001 tgaacttcgc ttcgaacaac tacctcggcc tcacgcatca cccgcgcgtg atcgacgcgt
     3061 ttgactcggc cgcaaaacgc gacggcgtcg gcagcgccgc cgcggcgctc gtgaccggat
     3121 acaccgatgc ccacgcgtcg gccgagcgcg cgatcgcggc ttggaaaggc agcgagtccg
     3181 cggtgatgct cagcagcggc tacgccgcca acctggcggc ggtgcaggcg atcgccgctg
     3241 tcgccggggg tgagcggcgg gtgcggttcc tgctcgacaa gctcgcccac gcgtcgctga
     3301 tcgacgccgt ccgcggcagc ggcgcggcgt atcgcgtgtt cccgcacaat catctcgaga
     3361 aactcgagcg gctgctggtt gaagcggagc ccggccagct gcaggtgctc gtcaccgaat
     3421 cgatcttcag catggacggc gacgcggcgg atctggcggg gattgcgcga ttgaagcaga
     3481 agcaaccatt cgtgctgctg ctggacgaag cacatggcag cggggtgtac ggcgtcaacg
     3541 gcgcgggata tgcatccgag attggccttc agtcgatcgt cgatgtgacc atcgtgacgc
     3601 tgtcgaaagc catcggcgtc agcggcgggg ccgtctgcgc ctcgcgcgcg ttctgcgatg
     3661 cgctcatcaa cttcggccgg tcgtatatct actcaaccaa cgttcccccc gcggtcgcgg
     3721 cggcggcgga ggcttcgatt gcggtgatgc gcgacgaacc gcagcgccag cagcgcgtgc
     3781 gggcgctggc gagggatttt cgggcggggc tgatggagat ggggctcaga ttggcgccgg
     3841 gcggaccaca cgccgggttc ggaggtactc cgacgacccg gattctgccg cgcagtggag
     3901 atgaagaatc cgggtcgtcg cgagtacgct ccgaacccgg cgtggctggt gcgtattcag
     3961 aaggctcggc gtgggattcg ccgatcttgc cggtgatcct cggtggggaa tcagaaagtc
     4021 tcaaagccgc ggagcgcttg cgcgaggcgg ggttcctggt ggtggcgatt cgcccgccaa
     4081 cggtgccgcg ggggtcgagt cggctgcgca tcacgctgtc gagcgagcac cgcgatgagg
     4141 aagttcgcgg actgctggag gcgttgcagt cactgtaaag ctggatgtgt aaagccagcg
     4201 atggtatcgc cagttttaat agccaaagac cggcgatacc atcgcaggct ttgcatggcc
     4261 gactttactt tttcacgcca ccggtggctg cggcaggtgc gtgcggcgcg tcgcgcggcc
     4321 cacatgccca gcgctggcgc gatccacgcg ctgcatcgtc gccgccacca ggtcgatcga
     4381 cggttcaccc gccggcatct ggcccagcag cttcagcacg cgttcggcgg cggccaatcg
     4441 cttctggctg acggcgtgcg ccacgcgggt caccccgctg gtggccgcgc cgtggtccag
     4501 cagcagatcg accgctcgac ggtcttcttc ggagaacttt cgattcatga tcgcaccgtg
     4561 ttttcgcggc gatgacgaac tcgcgcgtcg tcgaagattt gcatttcgct tcgtcaccag
     4621 ccgatacccc gtggagatta gacagctcag ttgtcgcgtg acagtcgcag cgcttgcggc
     4681 gcggaacaca acgtcggccg gtccaaacgc caaccgaaaa actggcactt tctcctaccc
     4741 ggcgaaactg cgttctccat gacgcagccg cctcgtggcg gccccacggc caccgtaccc
     4801 cattccctac gacatggcat ggctggggtt cgccgagcgc catcgatctg cgaaatgggc
     4861 caccgccgcg tgcaggcggg acttcacggt tcccagcgga atatcgagga tatcgctgat
     4921 ttgcttgtac ggaaactggt ggaaatacga gagcaataaa atctcgcgca ggtgttccgg
     4981 catgttcatg acggtctgct tcaccttgtt ctgcagctcc tgttgctgca tcggttcatc
     5041 cggcaaatcc gtcgcgctct tcatcaagtc gatgaattca ctgctgtcat cgtcacccgg
     5101 gctgatcgat gcctgcagcg ggctgaccgg gcggcgcgcg ttggcgcgga tcaggtcgcg
     5161 cgccttgttg gccgcgatgg tgaacagcca cggccggaag cggcgctgcg tgtcaaagtt
     5221 ttcggcgctc tggtgaatct gcaggaacgt ttcctggaac acgtcttccg ccgcggcgcg
     5281 atcgccgagg aagcggatga ggaagtggaa cagctcgcgc tgatatcggc cgacgagttc
     5341 ggcgaagctg ctgcgatcac cactgcgata cgcggcgatc agctgctcgt cggtgcgctg
     5401 accacggtcc gactccacca tgccgggctg tgcggcagtc gccaacaagg gcgagctgtc
     5461 ggggttcatg tacttgccat gaccgtttcc gccgtgaacg ttcgtgcccg ccggtttggc
     5521 ggctgcagtc ttcgcgccgc cggcagcgtg accgttgccg ttggacttac cgttggacgg
     5581 tgaatgtccg ctgcgcgcct tgtggctttg tggccgagtg ctcttcattg caggcctcca
     5641 gcaaacccgc tatcgtgcga ccggtcaact gcaaaaagtg accatccacc gcccgcacaa
     5701 cgtacaaatg atatgccgtc ttctgtccaa catcctgccg acgcccccaa agttgcgttt
     5761 tttgctctga ccgctgaaca ggttgaccgt ctcgtgcaaa cgtggggctg gcctcgattt
     5821 cgcggccggc aggtgcggga atgggtatac cacaaaggcg tcgtcgatcc agagcaaatg
     5881 accaacctgg gtaaggccga tcgcgcgctt ttgacggcgc gattcgactt cggctcttcc
     5941 acgatcgtca accaccagag cagcagcgac ggcaccgaaa aactgctgct ggcctggccg
     6001 gacggcaaca acgctgagac ggtcatgatc cccgacggtc cccgccggac ggcgtgcgtc
     6061 agctcgcaag tcggttgccc cgcgggctgc aaattctgcg caagcggggt gaacggcgtg
     6121 aagggaaacc tgtcggccga tcagatcgtc gagcagatcg tgcgtttgaa tgcgttgttg
     6181 aaacgcggcg acgaacgcat caccaacgtc gtcttcatgg gcatgggcga gccgctggcg
     6241 aattacgcga acgtcatgca ggcgatccgc gtgctgcacg atcccggctg cctgaacatc
     6301 ggcgcccgca agatcaccgt ctccaccgtc ggcgtgccgc cgaagatgcg gcagctggcg
     6361 caggaagagc tgccgctcaa tctcgcgatc tcattacacg cacccaacga gccgctgcgc
     6421 aaggaactga tcccgtgggc ggagcatttc gcgctcgatg acatcctgaa cgccgctcga
     6481 tattacttcc agcagaccgg ccgggagatc acgctcgagt acatcctgct ggccggcgtg
     6541 aacgataaac ccgagcacgc gcggcagctg gccaagctct gcaagacgct gcgggcgaac
     6601 gtgaacctga tccgctacaa cgaggtcgcc ggcctgccct tcggccggcc gaaggcggac
     6661 gatgtgacgg tcttccagga catcctccgc aacaacggcg taaacgcgca cgtgcgcaaa
     6721 agccgcggcc gagatatcga cgccgcgtgc ggtcagcttc gacggaaaca gcaggagcaa
     6781 caagaacagc tcatcacgat tggaacacct gcttaacaaa gtaaaacagc agaatgaacc
     6841 acagatgcac acagatgaac acagatcaaa taaagggtca tctctcttct gctatctgtg
     6901 ttcatctgtg tgcatctgtg gttccatcgt cttctcgttc gaagtttcgc cgcggcttca
     6961 gcgccgttga actgctcgtc gtcatcggca tcctgctggt cctcgtgagc atcttcgtgc
     7021 cgtacctgct gaagatccgc gagatggacc atcgcgtgaa gtgcgccgac aaccttcgca
     7081 tcatccgcgc cggcttggcc caatattcga cgaccaacaa cggcgactac ccccgcgtcg
     7141 tctacgatac gacccacccc ggctacgcct gctacaccgg cgttgacggc ggcagcccgt
     7201 tcgccaagga cagcggcgtc caagctaacg acgtgacggc gtcgctgtgg ttgctggtgc
     7261 ggatgaagct tgttgagccg cgcgcgttca tctgcccgag caccagcgac gacatcgacc
     7321 caattgacga cgccagcggt cacgcggtcg gcgtcgagct tcgcggcaat ttccgcgggc
     7381 ctgacagctt gagttattcg tacgcgtcgc cgttcagcgc tgcgccgggc tatcgcttga
     7441 acgacacgca gcgctcgggc tttgcggtga tggccgacaa gaattccggc aacgcggcca
     7501 gcgtgccgtt cgacgcccag ccgctgcagt tcgccgccgc caactccgcg aatcacgggc
     7561 aggcggggca gaacgtgttg tacgccgacg gtcacgtgga ctttcaaccg acaccgtatt
     7621 gcggcgtcga gcgcgacaac atcttcaccg cactggcgct caagccgctg gagaaggggc
     7681 aggagtcgcc gccgaagttc agcagtggca tcaccagccg agatgtgagc ccggcgtggg
     7741 agtcggacag ctacctcgtt cccaccgcga gtgatcgggt gccgacgacg gggccgacga
     7801 cgagcacgac ggcggcatcg acgcaggccg cgaccgcgcc ggcgacggga ccggtgacgg
     7861 tgccgccacc accgcaagca ccgccgcccc ctcctccgcc gccgcctcct ccaactacct
     7921 tgccgacgac cgggccttcg actcgaccgt aatcctccgg gtgccccttc ggggctaccg
     7981 ctacgctagc acacagattg cgccttgggc gatgaaccat cgccggttgc caaagccgcc
     8041 gggctgtcct tacagtatcc gcccctgatc tgaaaaaacg aagttttttt gacggagtcc
     8101 atcaatgcac taccggcgtt tgggtgaggc gggcgtgaag ctgtcggaga tcggcctggg
     8161 cgggtggttg acgttcggca acgcgatgga agcgcagcag gcgcgcagtg tgatcgacaa
     8221 agcctttgac gtcggcatca acttcttcga caccgccgac gcgtatgccg gcgggcaggc
     8281 tgaggaagcc tggggcgagc tgttcaagag ccgcgtgcgc tccagctacg ttttggcgac
     8341 gaaggttttc tttccgacgg gccaaggcgc gaacgatcgc ggcttgtcgc gcaagcatgt
     8401 cttcgagaac tgtcacaaga gcctgaagcg attgcgcacg gattatatcg atctgtatca
     8461 gtgccaccgg ttcgatccgg aaacgccgct ggaggaaacg gtgggcgtga tggacgacct
     8521 ggtccggcag gggaagatcc tgtactgggg attttccgaa tggacggccg agcaggtttc
     8581 gcaatgcctg caggtgtgcg gcgaccgtta cgagcgcccg cgcagcagcc agccgcggta
     8641 tagcgcgctg gagcgctacg tcgagaagga cgtgatgccg ctgtgtcaca aggccggcat
     8701 cgggcaggtc gtcttctcgc cgctggcgca aggcgtgctg accggaaagt acaaaccggg
     8761 ccaggcgttc cccgccgaca gccgggcggc ggacgatcgg cagaaccagt tcatcaagcg
     8821 cttcgtcgat gacccgcagc tgctggagaa ggtgcaacgg ctggtgccgc tcgcggagga
     8881 acatcattgc tcgatgtcgc agctggcgct ggcatgggtc ctgcgccggg cggaagtgac
     8941 cgcgtgcatc atcggcgcga gcaagccgca gcagatcgac gagaacgccg aggcgagcgg
     9001 gatcaagtta agcgaagagg acgctcggcg gattgacgaa gtgctcagct aaaaccaaag
     9061 acgccaaccc aagccgcccg gcagagccgg gggcttttgg ttttgcggtt gatcgatcac
     9121 ccgcgatgta gcatccaacc ccatcgcccg atagaatctc cccgcctatg aagatcatcc
     9181 ttgccaatcc ccgcgggttc tgcgctggcg tgaacatggc catcgactgc gtcgatcagg
     9241 tcctgaaatt gaagggcccg ccggtttacg tctaccacga aatcgttcat aacaagcacg
     9301 tggtccagga ct
//
LOCUS       DATIMH010000851         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_202050, whole genome shotgun sequence.
ACCESSION   DATIMH010000851 DATIMH010000000
VERSION     DATIMH010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202050"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VLI90_14765"
     CDS             <1..256
                     /locus_tag="VLI90_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15520.1"
                     /translation="KSKLKQSTGTMSTAEAISVVNSGMALAAHFGDGKLKAADLASGL
                     VGAVLKDPVQDRMVFQEYLETVVKERDGWKDIYRACRDVV"
     gene            369..854
                     /locus_tag="VLI90_14770"
     CDS             369..854
                     /locus_tag="VLI90_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15521.1"
                     /translation="MPVYHFTLHAYRSWRADNRRGYVHHTDGLCKPDKEIADARDRLA
                     KFDEVTFDETIQRLLIRATLETCRRRNWRLHGAGNDETHLHLAISWKRFEPVDDVMNR
                     LKNALSFVLGKEIGPRGRKWFVRGGSRKRVNDSEHLDYLLDRYFPDHPGVFWREGFAV
                     P"
     gene            973..>2870
                     /locus_tag="VLI90_14775"
     CDS             973..>2870
                     /locus_tag="VLI90_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSV15522.1"
                     /translation="MAKSSAKEQMQQLAANLQQAAAANNSAPFKDLVASGRELDVLFY
                     AAEFGGTDIVRAILAAGADANKGESHLKPLAIAAEHGQTEVARLLMDAGADVNDVGLL
                     GPPICEAVGNGHVEIVRLLLEHGADPHVKYMGTTLLCVAAGRGRSAVVPLLLKAGLDP
                     NQTDGSNRARTPIMWAAEVGDVQIIRELLAAGSRVDPPPRAGPPKDKMEEVYLEKGSA
                     LTIAARHGRAEAVAELLKAGADPKFVDGEGRTAYDWARKSQNAQMLKALIDAGAAPIG
                     GATLGDLVAAAEAGDCEGIRAALKAGIGVDEKDKATYDSSSVVCGGLSALMLAARGGH
                     VDAVKLLLERGANVNALSDDIAHGARPALIYAVAAGQAATVKQLLDAGADLAIAGKAF
                     DGSTGTALHAAARKGDAEMVKLLIAAGAQPADKSGGKPPLHLAAARDHLDVISALLGA
                     KAKIDQKDREGETALHTAAGKGKLAAVKLLIARGAKVNITDRSGATPLYASPTNLSLR
                     ETSIDAKGNKNVKDPDDPIGVMKALLAAGADPSIATERHGTVLAHSVSHPQAVQLLLE
                     HKADPNITDAEGNSPLLMAIYYSNSESVRLLLEAGAKANIVNEKGVTPLDVALDRKMT
                     KIAEMIEKA"
BASE COUNT          599 a          898 c          919 g          454 t
ORIGIN      
        1 caagagcaag ctcaagcaat ccaccggaac catgagcacc gccgaggcca tcagcgtcgt
       61 caacagcggc atggctctcg ccgcccactt cggcgacggc aaactgaagg ccgccgactt
      121 agcatccgga ctcgtcggtg ccgtgttgaa agacccggtg caggatcgca tggtatttca
      181 ggagtatctc gagacggtcg tcaaggagcg cgacggttgg aaagacattt atcgcgcgtg
      241 cagggacgtg gtttaaggac atctttaata gccgcaggcg acagccagcg gaggcgctag
      301 ataatcagcc gggctggtac tcgaaacccc ccgctggctg tcgcctgcgg ctatcaatgg
      361 gagcagccat gcctgtttat catttcacgc ttcatgcgta tcgatcctgg cgcgccgaca
      421 accgccgcgg gtatgttcat cacacggatg gactgtgcaa gccggataaa gagattgcgg
      481 atgcgcgcga ccgtctcgcg aagttcgacg aggtgacttt cgacgaaacc atccagcggc
      541 tactgattcg cgccacattg gaaacgtgcc gccggcgtaa ttggcgattg cacggcgcgg
      601 gcaacgatga aactcatctg catctggcga tcagctggaa gcgattcgaa cccgttgacg
      661 acgtcatgaa tcgcctgaag aacgcgctga gttttgtgct gggcaaagag atcggcccgc
      721 gcgggcgcaa gtggtttgtc cgcggcggca gccgcaaacg cgtgaatgat agcgagcatc
      781 tggactattt gttggaccgg tacttcccgg accatccggg agtgttttgg cgcgaaggct
      841 tcgcggttcc gtaacccgcg cccccgctgg ctgtcgcctg cggctattaa agcgatcttt
      901 agtagccgca ggcgacagcc agcgggatcg ttcaaatctc aaccggctta gttatcgaac
      961 gaggagcacg acatggcaaa atcttccgcc aaagaacaaa tgcagcaact cgccgcgaat
     1021 ctacaacagg ccgcggcggc gaataattct gccccgttca aggacctcgt cgccagcggg
     1081 cgcgagctgg acgtgctctt ctacgccgcg gagtttggcg ggaccgatat cgtccgcgcg
     1141 atcctcgccg cgggcgcgga cgccaacaag ggcgagtcgc acctcaaacc gctggcgatc
     1201 gcggcggagc acgggcagac ggaagtcgcc cgcctgctga tggacgccgg cgcagacgtg
     1261 aacgatgttg gcctcctcgg tccgccgatc tgcgaggcag ttgggaacgg tcatgtggaa
     1321 attgttcgcc tgctgttgga gcacggcgcc gatccgcacg tcaagtacat gggcaccacg
     1381 ctgctgtgcg ttgccgcggg gcgcggtcga tcagctgttg tgccgctact acttaaggcg
     1441 gggctggacc cgaaccaaac cgacggcagc aaccgcgccc gcacgccaat catgtgggcg
     1501 gcggaagttg gtgacgtgca gatcatccgc gagctgctcg cggctggctc ccgcgttgat
     1561 ccacctccgc gcgcagggcc gccaaaggac aagatggagg aggtttattt ggaaaagggg
     1621 agcgcgctaa cgatcgccgc gcggcatggc cgtgccgaag cggtggccga gctgctcaag
     1681 gcaggcgcgg atccaaagtt tgtcgatggc gaagggcgca cggcatacga ctgggcgcgc
     1741 aagagccaga acgcccagat gctcaaggcg ctgatcgacg ccggcgctgc cccgatcggt
     1801 ggcgcgacgc tcggcgacct tgtggccgcc gccgaggccg gcgattgtga aggcatccgc
     1861 gccgcgctga aagctggtat cggcgtggac gagaaagaca aggcaacgta cgacagcagc
     1921 agcgtcgtct gcggcggcct gagcgcgctg atgctcgccg cccgcggcgg gcacgtcgac
     1981 gccgtaaaac tgctgctcga acgcggcgcg aacgtgaacg cgctgtccga cgacatagcc
     2041 catggcgccc gcccggcact gatctacgcc gtcgccgccg gacaggcggc caccgttaaa
     2101 caactgctag acgccggtgc cgatctcgcc atcgccggaa aggcattcga tggcagcacc
     2161 ggcaccgcat tgcacgccgc cgcccgcaag ggtgacgcgg aaatggtcaa gttgctgatc
     2221 gccgccggcg cccaaccggc tgacaagtcg ggcggcaaac cgccgcttca tctggccgcc
     2281 gcccgtgatc acctggatgt catcagcgcg ctgctcggcg ccaaagccaa gatcgatcag
     2341 aaagatcgcg aaggcgaaac ggcgctgcac accgccgccg gtaagggaaa actcgcggcg
     2401 gtgaaactgc tgatcgcccg gggggcgaag gtcaacatca ccgacaggag cggcgcgacg
     2461 ccgctctatg ccagtcccac caacttgagc cttcgcgaga cgagcatcga cgccaaaggc
     2521 aacaagaacg tgaaggaccc cgacgatccg atcggcgtga tgaaagcgct gctcgccgcc
     2581 ggggccgatc ccagcatcgc caccgagcgc cacggcaccg tgctcgctca ttccgtcagc
     2641 catccgcagg cggtccagct gctgctggaa cacaaggccg acccgaacat cactgatgcc
     2701 gaaggcaatt ctccgctgct gatggcgatc tactacagca attccgagtc cgtgcgcctg
     2761 cttctcgagg ccggcgcgaa agccaacatc gtcaacgaaa aaggcgtgac gccgctggac
     2821 gtcgcgctcg accgaaagat gacgaagatt gccgagatga tcgagaaagc
//
LOCUS       DATIMH010000852         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5405516, whole genome shotgun sequence.
ACCESSION   DATIMH010000852 DATIMH010000000
VERSION     DATIMH010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5405516"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VLI90_14780"
     CDS             complement(<1..587)
                     /locus_tag="VLI90_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15523.1"
                     /translation="MSKEQAKPQTPIDVLVLGEHPAGYLVAALLRHKTKLRIVHAGIP
                     GEDDVKDRLVTINPQLFDLHPLLGGLKRKLNLTGVHGISFFADDPETHSEYRAKSIVS
                     YVARFKSVRDEMAKIADAEGVERVTPSAIKIHRLDETGLDATLGKTTVRATALVLAGQ
                     LAPDQHALLGLPDSWGPDVVHRFTFTRCKAAKAIDT"
     gene            1043..1741
                     /locus_tag="VLI90_14785"
     CDS             1043..1741
                     /locus_tag="VLI90_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSV15524.1"
                     /translation="MTAWPAAVLFDFDGVIVNSEPLHFDAFQQTLATEHIELSEDEYF
                     RELVGFDDRGAFAHVFKKHGRGLDPKTFLRVMTRKSEMMMELIHLRRYAALGGVEEFV
                     RALWRQYPLAIVSGALREEIEAMLEGVALRDCFPTIVAAEDVTAGKPDPQGYLLGVKL
                     LRERSGKKIEPKDCLVVEDAPSVIKSVRAAGFKTLAVASSYPLERLSDANYAVRRLDI
                     AEVKKQIPQLKVQI"
     gene            1876..2187
                     /locus_tag="VLI90_14790"
     CDS             1876..2187
                     /locus_tag="VLI90_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15525.1"
                     /translation="MRSWALALILLAFQATPAVAQESGASTTLPADVRPADADADTHP
                     VVSTDATWAGSVVILIGVMFVSAMVIGPIVRAEMTTDVPVAFSHDEDPSHHAGGHGGH
                     H"
BASE COUNT          442 a          736 c          768 g          468 t
ORIGIN      
        1 gtgtcgattg ccttggccgc cttgcaacgc gtgaacgtga agcgatgtac gacgtccggt
       61 ccccacgaat ccggcagccc cagcaaggcg tgctgatcgg gcgctaattg ccccgccagc
      121 accaacgccg tcgcgcgcac cgtcgtcttg cccagcgtcg catccaaacc cgtttcatcg
      181 agccgatgaa tcttgatcgc gctgggcgtc acgcgctcga cgccttcggc gtcggcgatc
      241 ttcgccatct cgtcgcgcac gcttttgaac cgcgcgacat atgagacgat ggacttggcg
      301 cggtactcgc tgtgcgtttc cggatcatcc gcgaagaagc tgatgccgtg cacgccggtc
      361 aggttcagct tgcgcttgag cccccccagc agcggatgca gatcgaacag ctgcgggttg
      421 atcgtgacca gccggtcctt cacatcgtct tcaccgggga tgcccgcgtg gacgatgcgc
      481 agcttggtct tgtgccgcag caacgccgcc accaggtaac cggcggggtg ctcaccgagg
      541 accagaacat caatcggcgt ttgcggcttc gcttgctctt tagacatggc ggatcagagt
      601 cggatcatac ccgcccgctc gaaggattgt taatcaacgc ttacggcttg cgttaaattg
      661 ccggcctctc gaaccccggc ggcacaagcg ttgcatcctt tggcccatca gacttaaagc
      721 ccctcagagg gcaaggagca accatgactc gcgttcttct aaccgtgttg tcgctcggtc
      781 ttttcgccgc cgccctgacg ggctgccatg cttcgggcga tgttgatggc gtcagctcga
      841 tcggtgtgat gaagtaaatt ttcgcgcgca ggcgcgcaca cgcgctccgc gcatcgcgcg
      901 cggttcgcgt atctcatcgc tatttgtaca gccggcgacg catggcccgc tcccgaaggg
      961 atctgacgat ctcaacgacg tgtcgggatt cggcatgctc gccggctttt ttgctgcgcg
     1021 tcgatccgct accttacccg cgatgactgc ctggcctgcc gccgtgttgt tcgacttcga
     1081 cggcgtgatc gtcaacagcg agccgctgca cttcgacgcg ttccaacaga cgctcgccac
     1141 cgagcacatc gagcttagcg aagacgaata cttccgcgag ctggtcggct tcgacgaccg
     1201 cggggcgttc gcgcacgtct tcaagaagca cggccgcgga ttggacccca agacgttttt
     1261 gcgggtgatg acgcgcaaga gcgaaatgat gatggagctg atccacctgc ggcggtacgc
     1321 cgcgttgggc ggggtcgaag aatttgtccg cgcgctctgg cggcagtacc cgctggcgat
     1381 cgtctccggc gccctgcgcg aagaaatcga agcgatgctg gaaggcgtgg cgctgcgcga
     1441 ctgttttccg acgatcgtcg ccgccgagga cgtgacggcg ggcaaaccgg acccgcaggg
     1501 ttatctgctg ggtgtaaagt tgcttcgcga gcggtcgggg aagaagattg agccgaagga
     1561 ttgcctggtg gtggaggatg cgccgagcgt gatcaaatca gtgcgcgcgg ccggcttcaa
     1621 aacgctcgcg gtggcgagca gctatccact ggaacggctg agcgatgcga actacgcggt
     1681 ccgccggctg gacatcgcgg aagtgaaaaa gcagatcccg cagttgaagg tgcagatctg
     1741 acgacgccgg gttcggagca gcctgcctgg agcttgtcga agagtactgg cgacgacccg
     1801 gatcggacgt cgaaatacag aatgactgcc gagtctgccg tcttcgattc cgcattcaat
     1861 gcaaacggag cacacatgcg atcatgggca ctggcgctga tcctgctggc gtttcaagca
     1921 acgccggccg ttgcacagga aagcggcgca tcgacgacgt tgccggcgga tgttcgcccg
     1981 gcagacgcgg atgccgacac gcatccggtg gtctccaccg acgccacctg ggccggcagt
     2041 gtcgtcatcc tgatcggcgt gatgttcgtc tcagcgatgg taatcggccc gatcgtgcgg
     2101 gcggagatga ccacggacgt gcccgtggca ttttcgcatg atgaggaccc gtcccatcac
     2161 gctggtgggc acggcgggca ccattgaggg gatcgattgt catcctgagc gaagcacgta
     2221 caggtgcgca gtcgaaggat cttgtcgaga agtatgtgaa gcgcatctgt gcgcagcggc
     2281 gtcgcagtgg cttgcccgtc gcggataagc cgcggcgaat ccgccaaggg caagcgacgt
     2341 ccagaactat tcgagaaatg tcagttccgc cctgtcgtgg caagatcctt cgactccggc
     2401 gagtacgcct gcgc
//
LOCUS       DATIMH010000853         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4827922, whole genome shotgun sequence.
ACCESSION   DATIMH010000853 DATIMH010000000
VERSION     DATIMH010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4827922"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VLI90_14795"
     CDS             complement(<1..536)
                     /locus_tag="VLI90_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15526.1"
                     /translation="MPTKSLRVAVLCLFALPLLAAATEENHPPIHVNHPALKSLSWQL
                     AAEGATFRGYSALDMIDLLHSLDCHHIELAPGQPLSGDHAGVVIGQEMSAADVDALLA
                     KLKSVKMDIVSYGPVQLKDDETARAVFEFAKKLKAKNVVISQSAVTMESLDGIASQAK
                     VKAALLLPGAVGSAKEKGG"
     gene            634..1179
                     /locus_tag="VLI90_14800"
     CDS             634..1179
                     /locus_tag="VLI90_14800"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSV15527.1"
                     /translation="MSWIENRFEEGLIVTSLDFAINWARKNSLWPMTFGLACCAIEMM
                     AVGASRFDLDRFGAGAFRATPRQSDLMIVAGTVNYKMASRLKRLYHQMPDPKYVIAMG
                     ACTVGGGPYFKYGYNVVKGVDLIVPVDVYVPGCPPRPEALMEGIMRLQDKISAKTIGL
                     PRGARAAAADRPLIAPSPAAI"
     gene            1219..1539
                     /locus_tag="VLI90_14805"
     CDS             1219..1539
                     /locus_tag="VLI90_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV15528.1"
                     /translation="MLEDSETKVTAVASAEEALAALSRRKFDVIVSDIRMPDRDGYDF
                     VRELRNIPPTAGGRTPAVALTAFARAEDRIRAMLAAFDVHIGKPVEPNKLCAVVARLS
                     GRNY"
     gene            1976..>3702
                     /locus_tag="VLI90_14810"
     CDS             1976..>3702
                     /locus_tag="VLI90_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV15529.1"
                     /translation="MRKGSTKSAAKLAMYSSAVVMAAMSVPMARAAVTVDGLLDADYG
                     TPRATQTVNTSFGDNTNVDGNTANGSELDAAYGVVQSGTGMLNLFFAGNVETNFNHLN
                     IFIADGRSGQSTLNAAGGGSNLNNMNGSKFAQGFTATYALDINGGGQGVNPPTWFANS
                     YDLRQAVAPATFLGSYTPPSTTAQHDTGQFGTSGVFFGVDNSNTAGVIGDGTGSAADP
                     NAANAVATGLEISIPLSLLGNPHGSLKVLADINGGGNGFLANQFLPGLPVNSGSVGGS
                     NGKSSFDFSASNTEFFSVGIPAGNNTNGNWLPGGGGSWSTAANWSTNPSAPNFAGDSA
                     TFGSATTASTVTLDGNKTVGQISFNSASSYTIAAGASGTLSIDDTGDSGGFDPSIDVS
                     LGNHTISAPLSLAATTAFGVTDNTSLTVSGNISGAGGISKAGAGSVILSGSNSFGGGI
                     TVLAGTVELDSASAASDTPINLGDPSTDLVPATLAIGVSGLTIGNHITTNHDLSATNT
                     FRTISGTYTSGNATVSSTIDVNGGINFFLPNAGTSLTVTGLIEAGTDTHSSARHSIHV
                     SGLGTVILT"
BASE COUNT          729 a         1205 c         1114 g          654 t
ORIGIN      
        1 ccgccttttt ccttggcgga cccgaccgcc cccggcagca ataacgcagc cttcaccttg
       61 gcctggctgg cgataccgtc cagcgattcc atcgtcaccg ccgactgcga gatcaccaca
      121 ttcttcgcct tcaacttctt cgcaaactca aagaccgcgc gggccgtctc gtcatctttc
      181 agttggaccg gcccatagct gacgatgtcc atcttcaccg acttcagctt cgccagcagc
      241 gcatccacat ccgcggcgga catctcctga ccaatgacga cgccggcgtg atcgccggaa
      301 agcggctgcc ccggcgcgag ctcgatgtga tggcaatcca gcgaatgcag caggtcgatc
      361 atgtccaacg ccgaatagcc gcggaacgtc gcgccttcgg cggccagttg ccagctcagc
      421 gatttcagcg ccggatggtt aacgtgaatt ggcgggtgat tttcctccgt cgccgcggcc
      481 agcaggggta acgcgaacag gcaaagcacg gcgacccgaa gcgatttggt tggcatgttg
      541 caatatgctg tcgaaaagcc ttgagacttc aagggagtcg ctcgccaagc ctatacttga
      601 gttgtcggcg gcgcaacgga taatagaggc aacatgtcct ggatcgaaaa tcgctttgaa
      661 gaaggtctga tcgtcaccag cctggacttc gccatcaact gggcgcggaa gaactcgctg
      721 tggccgatga cgttcggcct ggcgtgctgc gcgatcgaga tgatggcggt cggcgccagc
      781 cgattcgacc tggaccgttt cggcgccggc gcgttccgcg ccacgccgcg gcagagcgat
      841 ctgatgatcg tggccggtac ggtgaattac aaaatggcga gccggctcaa gcggctatac
      901 caccagatgc cggacccgaa gtacgtcatc gcgatgggcg cgtgcaccgt gggcggcggg
      961 ccgtacttca agtacggcta caacgtcgtc aaaggcgtgg acctgatcgt gccggtggac
     1021 gtgtatgtcc cgggctgtcc cccgcggccg gaggcgctga tggaaggcat catgcggctg
     1081 caggacaaga tcagcgccaa gacgatcggc ctgccccgcg gcgcgcgagc ggcggcggcg
     1141 gatcgtccgc tgatcgcgcc gtcaccggcc gcaatctaag ccagagccgg cccgtcctgg
     1201 ggtcggacct gggccgggtt gctggaagac tccgagacga aagtgacggc ggtggcgtcg
     1261 gcggaagaag cgctggccgc gctgtcgcgc cggaagttcg acgtgatcgt cagcgacatc
     1321 cgtatgccgg atcgcgacgg ttatgacttc gtccgcgagc tgagaaacat tccccccacc
     1381 gccggcggcc gcacccccgc ggtggcgctg acggcgttcg cccgcgcaga agatcgcatc
     1441 cgcgcgatgc tggccgcctt cgacgtgcac atcggcaaac cggtggaacc gaacaagctt
     1501 tgcgccgtcg tcgcgcgctt gagcgggcgg aattactgac gaagcttcgc gccacatcgg
     1561 gtggtctttc ttagcgcggg cgttagctta agggttactc aaaatactca gctacaagtc
     1621 gtcctggaag ctctaagttc ttgctcgaac aaggcctaaa tccgaagcgg ctccatcgca
     1681 aatctctcga cccatccaca ccggcaaata ccgcactttt gcattgaact gcgtgcggtg
     1741 aagtgttaac ctacaggtgg aggtgcggtc tacgccattt ggttgttggt gttttgtcgc
     1801 gaccaatccc gtcctgattt tcaagagaaa accatgtttt gcagctcgat catcccggag
     1861 tggcggccat cgcgaaacag ggttaaccgt ttcaatgaac tgctcaacat tccggtcatc
     1921 gcgtggcgcg cgtgagatgc gcgcgcgacg cctttcaatt acgggagcgt gtgccatgcg
     1981 gaagggttca acgaagtcgg cagcgaagtt ggcgatgtat tccagcgccg tggtgatggc
     2041 cgcgatgtcc gtgccgatgg cgcgggcggc ggtgacggtc gatggtctgc tcgacgcgga
     2101 ctacggtacg ccgcgggcga cgcagacggt caacactagc tttggcgaca acacgaacgt
     2161 cgatggcaac accgccaacg gatcggaact cgacgcggcc tacggcgtgg ttcaatccgg
     2221 caccggcatg ctgaacctgt tcttcgccgg caatgttgag acgaacttca atcatctcaa
     2281 catcttcatc gccgacggcc gcagcgggca atccaccctc aacgctgctg gcggcggatc
     2341 gaacttgaac aacatgaacg gctcgaagtt cgcccaggga ttcaccgcca catacgcgct
     2401 ggacatcaat ggcggcggtc agggcgtgaa cccgccgacg tggttcgcca acagctatga
     2461 cctgcgccaa gccgttgcgc cggcgacgtt cctcggttca tacacaccgc cttcgacaac
     2521 cgcccagcac gacaccggcc aattcggtac ctccggtgta ttcttcggcg tcgataattc
     2581 caacaccgcc ggcgtgattg gcgacggcac gggctccgcc gcggacccaa acgccgccaa
     2641 tgccgtcgcc accggactgg agatctcgat cccgctatcg cttctgggta acccgcacgg
     2701 gtcgctcaag gtcttggccg atatcaacgg cggcgggaat ggtttcctgg cgaaccagtt
     2761 tctaccgggg ttgcccgtga attcgggcag cgtcggtggg agcaacggca aatccagctt
     2821 cgacttctcc gccagcaaca cggaattttt cagcgtcggg atccccgccg gcaacaacac
     2881 caacggcaat tggctgcccg gcggtggcgg aagttggagc accgccgcga actggagcac
     2941 caatccctca gcccccaact tcgccggtga ctccgcgacc tttggcagcg caaccaccgc
     3001 atccaccgtc acgctcgatg gcaacaagac cgtcgggcag atcagcttca attccgccag
     3061 cagctatacg atcgcagccg gcgccagcgg cacgctgagc atcgacgaca ccggtgactc
     3121 cggtgggttc gatccgtcga tcgatgtttc gctgggcaac cacacgatct ccgcgccgct
     3181 gagcttggcc gccaccaccg cgttcggcgt aaccgacaac acatcgctga ccgtgagcgg
     3241 caacatcagc ggcgccggcg gcatcagcaa ggccggcgcg ggcagcgtca tcctcagcgg
     3301 gagcaactcg ttcggtggag gaatcaccgt gctcgcgggg acggtggagc tggactcggc
     3361 cagcgccgcc agcgatacgc ccatcaacct cggtgacccc tcgaccgacc tggtgccggc
     3421 gacgctcgcg attggcgtca gcggtctcac catcggcaat cacatcacca ccaaccacga
     3481 cctgtcggcg accaatacct tccgcacgat cagcggcacc tacaccagcg gcaacgccac
     3541 ggtcagcagc acgatcgacg tgaacggcgg gatcaacttc ttcctgccca acgccgggac
     3601 atccctcacc gtcaccggtc taatcgaagc tggcaccgac acgcacagca gcgcgcggca
     3661 cagcattcac gtgagcggcc ttggcaccgt gatcttgacc aa
//
LOCUS       DATIMH010000854         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3092155, whole genome shotgun sequence.
ACCESSION   DATIMH010000854 DATIMH010000000
VERSION     DATIMH010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3092155"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VLI90_14815"
     CDS             complement(<1..295)
                     /locus_tag="VLI90_14815"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HSV15530.1"
                     /translation="MPHEIELKLELPANDLDRLAMLPWLNALTAGPGKRAELVSVYYD
                     TESFQLREHGVSLRVRSDGNRYVQTIKAPGKTRRGPLARQEWEHEIGDAAPNLR"
     gene            430..903
                     /locus_tag="VLI90_14820"
     CDS             430..903
                     /locus_tag="VLI90_14820"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HSV15531.1"
                     /translation="MPKTSPRQRKTIGRVMHEYEHGELKSGADGKAGPVRNRRQAIAI
                     ALHEAGASNKESGGKNRQNLARTKRKEARGQTAQQEKEGKSHVGASGRRESSRAMGGE
                     NAKKTTARGRESARSSRGRDGEQTTRSALYAKAKARDIPGRSKMNKQQLERALSH"
     gene            complement(916..>1921)
                     /locus_tag="VLI90_14825"
     CDS             complement(916..>1921)
                     /locus_tag="VLI90_14825"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV15532.1"
                     /translation="AVLATGIVPLVFALVPKSLPVLVVMVVQAAAGSLFTPAIAAISL
                     ALVGRDGLGARVGRNGRYGSIGAGLGAAMMGACGYLGSPRLAFLVAALLTIPALLAVR
                     AIGPDQVEVVPMTRREKRPNPLTPFVLLRDRRLLIFALCVALFQVASIAVLQLAAVDV
                     TGRLGARGALVIAAFMIVPQVVVAIVSPWIGQAADRWGHRTMLIAGFATVPIRGALFA
                     LVRNPYVLVPVQVLEGAGGAAFGVMMPLVAADLTGGKGHYTLCLSLLGLAAGAGTAVS
                     TALAGWSADRFGRVTAFWVLAGVGVAALAVVALAMPETRPRASSGRGPKPDAARPGLA
                     "
BASE COUNT          386 a          647 c          646 g          242 t
ORIGIN      
        1 gccgcaaatt cggcgcggcg tcgccgattt cgtgttccca ctcttgccgg gccagcggtc
       61 cacgacgggt cttcccgggt gccttgatcg tctgcacgta acggtttcca tcggaacgaa
      121 cgcgaaggga cacgccgtgc tcgcgaagtt ggaacgattc ggtgtcgtaa tacaccgaca
      181 ccagctccgc gcgctttccc ggccccgccg tcagcgcgtt gagccagggc agcatcgcga
      241 gacggtcgag atcgttggcg gggagttcca gcttgagttc gatttcatgt ggcatgggcg
      301 cgggccagtc aaatgcgtgt ccagttcaac gtccccgcct cgccaaggtt tcggtgagcg
      361 ctcgccggcg cgcatcggaa cccggccgac gtgccggcat ttggcggtca taacagggag
      421 ttagctaaga tgccgaagac ttcaccccgt cagcgcaaaa caatcggtcg ggtcatgcac
      481 gagtacgagc atggcgaact caagagcggt gcggacggca aggccggtcc ggtcaggaac
      541 cgccgccagg caatcgcgat cgcgttgcat gaggcaggcg cgtcgaacaa ggaaagcggt
      601 ggaaagaacc gtcagaacct cgctcgcacg aagcggaagg aagcgcgcgg gcagacggcg
      661 cagcaggaga aggagggcaa atcgcatgtg ggcgcttccg gacgccggga aagctcacgc
      721 gccatgggtg gagagaacgc caagaagacg acggcgcgcg ggcgtgaaag cgcgcgttca
      781 tcacgagggc gggatggcga acagacgacg cgatccgccc tctatgccaa agcgaaggcg
      841 cgggacattc cgggccggtc gaagatgaac aagcagcaac tggagcgtgc cctgtcgcac
      901 tgatcatagg caggatcagg ccagccctgg gcgggcggcg tcaggcttcg gaccgcgccc
      961 ggacgaggcc cttggccgcg tttccggcat cgccaacgcc accaccgcca aggccgcgac
     1021 gcccactccg gccagcaccc agaacgcggt gacccggcca aaccggtcag ccgaccatcc
     1081 ggcgagcgcc gtgctgaccg ccgtgccggc gccggccgcg aggcccagta ggctcaggca
     1141 caaggtgtag tggcccttgc cgccggtcag gtcggcggcg accaggggca tcatgacgcc
     1201 gaatgccgcc ccgccggcgc cttccaggac ctgcaccggc accaatacat agggattgcg
     1261 caccaacgcg aacagcgccc cgcgaatggg cacggtggcg aaaccggcga tcagcatcgt
     1321 tcggtgcccc caacgatcgg ccgcctgacc gatccagggc gaaacgatcg cgacgaccac
     1381 ctgcgggacg atcatgaagg ccgcgatcac cagtgcgccg cgtgcgccca accgtccggt
     1441 gacgtcgacg gccgccagtt gcagcaccgc gatgctggcc acctggaaca acgccacgca
     1501 cagcgcgaag atcagcagac gccggtcacg cagcaagacg aagggcgtga gcggattggg
     1561 ccgcttttcc cgccgcgtca ttggtaccac ctcgacctgg tccggtccga tcgcacgcac
     1621 cgcgagcagc gccgggatgg tcagcagggc cgccaccagg aaagcaagcc gcggggagcc
     1681 gaggtaaccg caggcgccca tcatcgcagc gcccaacccg gcgccgatcg atccgtatcg
     1741 gccattgcgg cccacgcgcg cgcccagacc atcgcgtccg accagagcca ggctgatcgc
     1801 ggcgatcgcg ggggtgaaca aactgccggc cgccgcctgc accaccatga ccaccaggac
     1861 cggcaggctt ttgggcacga gggcgaacac aagcggcacg ataccggtgg cgaggacagc
     1921 c
//
LOCUS       DATIMH010000855         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_783484, whole genome shotgun sequence.
ACCESSION   DATIMH010000855 DATIMH010000000
VERSION     DATIMH010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783484"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VLI90_14830"
     CDS             complement(<1..264)
                     /locus_tag="VLI90_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15533.1"
                     /translation="MSTDRNFEDKLETWRLEAKHGRAVTGRGVDVTGGPIPKRPGYFG
                     EAVVRPPVWTWEIPLYFFIGGAAGMAPLLACAGFFLHMLELSRV"
     gene            complement(278..1150)
                     /locus_tag="VLI90_14835"
     CDS             complement(278..1150)
                     /locus_tag="VLI90_14835"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSV15534.1"
                     /translation="MIGEGTASTNQRRHSYVEDHVEVGFFTDPSVCIGCKACEVACKE
                     WNEVPNDGYTWTGNSYDNTGHLGASTWRHVLFVEQDLSKGNQITGPMSLGSGKDDDPL
                     RWIFLSDVCKHCENAGCLESCPTGSIVRTEVGSVLVQDDVCNGCGYCVVNCPFGVIDR
                     RPEPLPNAGGAFKCTFCYDRQKSGLTPACAKVCPTESIVFGRLDDLRARGAERVKTLQ
                     AEGYGDAQIYDPTDTSVAGIHAFFLLLGGPHAYGLPSKPQVPTIYLKSAWMAAGATAL
                     GAIAAVAAAFAFFG"
     gene            complement(1157..>2211)
                     /locus_tag="VLI90_14840"
     CDS             complement(1157..>2211)
                     /locus_tag="VLI90_14840"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HSV15535.1"
                     /translation="GSTASGCWIYCGCYASDGVNKPLRREPNDYLGHGWGFAWPTDRR
                     IIYNRASAAPDGTPWSEAKKLVWWDPAEKKWTGQDVPDFTPTKSPDYQADSSAEGLEA
                     LPGDAPFILHEDGLGWMYVPKGLQDGPIPTHYEPLESPVSNPLYPRDTNPVVNWFTRQ
                     DNRFAPPGDPRFPYVLTTYRLTEHHTAGGMSRFLPHLAELQPEMFAEISPELASELGI
                     ANADRISIVSLRGAIEARALVTRRIRPLQLDGRRIHQVAIPFHFGSAGPVRGDAANDL
                     MAISGEPNVIIMETKSLVCNIVPEPLPRGRDFLPWLNKYAPQDGPEVLHPEQPPVGAP
                     GGGKMTPGHGQHGKSG"
BASE COUNT          403 a          689 c          701 g          418 t
ORIGIN      
        1 gacccgggac aactccagca tgtgcaggaa gaatccggca caggcaagaa gcggcgccat
       61 tccggcggca ccgccgataa agaagtagag cggaatttcc cacgtccaaa ccggtggcct
      121 aacgactgcc tcgccgaaat aaccggggcg tttcggaatc ggtccgccgg tgacgtcgac
      181 gccgcggccg gtcacggcac ggccgtgttt cgcctcgagt cgccaggtct cgagcttgtc
      241 ttcgaagttg cggtcggtgc tcatcgcagg gctggggtca tccgaagaaa gcgaaagctg
      301 cagccacggc cgcgatcgct ccgagcgcgg tggcgccagc ggccatccag gccgatttca
      361 aataaatcgt cggcacctgc ggcttggacg gaagcccgta ggcgtgcggg ccgcctaaca
      421 acaggaagaa agcgtgaatg cccgcgaccg aggtgtcggt cggatcgtaa atttgcgcgt
      481 cgccatagcc ctcagcctgc aaggtcttga cccgctcggc cccccgcgcg cgcaaatcgt
      541 ccagccgccc gaagacgatc gactccgtcg ggcaaacctt cgcgcaggcc ggagtgagtc
      601 cgctcttttg gcgatcgtaa cagaaggtgc atttgaatgc gcccccggcg ttaggcagcg
      661 gctcggggcg tctgtcgatc acgccgaagg gacaattgac gacgcaatag ccacagccgt
      721 tacagacgtc gtcctgcacc agcacgctgc cgacttcggt gcgcacgatc gagccggtcg
      781 ggcacgactc caagcaaccg gcattttcgc agtgtttgca aacgtcggaa agaaatatcc
      841 agcgcagcgg gtcgtcgtcc tttccactgc ctaacgacat cggcccggtg atttgatttc
      901 ccttgctcag gtcctgttca acgaagagga catgtctcca ggtggaggcg ccgaggtggc
      961 cggtgttgtc gtaggagtta cccgtccagg tgtagccgtc gttcggcacc tcgttccatt
     1021 ctttgcaggc gacctcgcag gccttgcagc cgatgcagac gctcgggtcg gtgaagaagc
     1081 cgacctcgac gtggtcctcc acgtaactgt gccgacgctg attcgtgctc gctgtgcctt
     1141 cgccgatcat tctttcttat cctgatttcc cgtgctgacc gtggcccggg gtcattttcc
     1201 cgccgcccgg cgcgccaacc ggcggctgct caggatgcag cacttcaggg ccatcctgcg
     1261 gcgcgtactt gttcaaccaa ggtaggaaat cgcggccgcg cggcaacggt tcggggacga
     1321 tgttgcagac gagcgacttg gtttccatga tgatcacatt cggctcgccc gagatcgcca
     1381 tcagatcgtt cgccgcgtcg ccgcggactg gtccggctga tccgaaatga aacggaatcg
     1441 caacctgatg aatcctgcgc ccgtcgagct ggagcggccg gatgcgccgg gtgaccagag
     1501 cgcgcgcttc gatcgcgcca cgcaacgaga cgatgctgat ccgatcggca tttgcgatgc
     1561 ccagctcgct cgcaagctcg ggtgaaatct cggcgaacat ctccggctgc agctcggcca
     1621 aatgcggcag gaagcgactc atgccaccgg cggtgtgatg ttcggtaagg cggtaggtcg
     1681 tgagaacgta gggaaaacgc gggtcgcccg gcggcgcgaa gcggttatcc tggcgcgtaa
     1741 accagttcac cactggattg gtgtcgcgcg ggtagagcgg atttgagacg ggcgactcca
     1801 gcggctcgta atgggtcggg atcggaccgt cctgcagtcc cttcggcaca tacatccagc
     1861 cgaggccgtc ttcgtgcagg atgaatggcg catcgccggg caacgcttcc agtccttccg
     1921 cggagctgtc ggcctgataa tccgggcttt tggtaggcgt gaaatccgga acgtcctggc
     1981 cggtccattt cttttccgct ggatcccacc agacgagctt cttcgcttcg ctccacggcg
     2041 tgccatcggg cgcggcgctg gcgcggttgt agataatccg gcgatcggtt ggccaggcga
     2101 agccccagcc gtgaccgagg tagtcgttag gctcgcgtcg cagcggtttg ttcacaccgt
     2161 cgctcgcgta gcagccgcaa taaatccagc agccgctcgc ggttgagccg t
//
LOCUS       DATIMH010000856         1595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5406016, whole genome shotgun sequence.
ACCESSION   DATIMH010000856 DATIMH010000000
VERSION     DATIMH010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5406016"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1595
                     /locus_tag="VLI90_14845"
     CDS             <1..>1595
                     /locus_tag="VLI90_14845"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HSV15536.1"
                     /translation="GVCLAGAVQDFLVLSASIRRNGKSLAQIAFHDIGPIAGVSAMIA
                     ILFILVIALAGLGKVVVKALGGEPAYHVPGSSWGTFTIAATIPIALFIGLYMNRLRRG
                     RVLEASIVGGLLTIGATVLGGWVAQRPIGHYFNLTAGQVVWAMAVYGFIAAVLPVWVL
                     LVPRDYLSSFLKIGTIVLLVGGVIVANPTLRAPAINSVFTSGGPVVKGKIFPFLFITI
                     MCGAISGFHALVSSGTTPKMVKRESDARMIGYGAMLMEGLVGVVAMIAAATLPVGDYY
                     AMNTEIAAMPAFHDQIIKIPGGGIEHVADYEVLTQESLRGRTGGAVTLAVGMAEVFDH
                     SLGTLAGGARWLWKYWYHFAIMFEALFILTTIDAGTRIGRFLLQEMLGKIHPKLGQTN
                     WWPSAYLSTAIIVGGWAYFINADSFAAIWAMFGVANQMLAVIALAVVSVYLVNEGRAK
                     YLWVTVVPLAVVMTTTSTAAVQILIGKVDGIRTQIAKDVLTAAERALLMNSAITAALL
                     VAMMACTVLIIASAGVRIAGVRRP"
BASE COUNT          264 a          427 c          577 g          327 t
ORIGIN      
        1 ggggtttgcc tggcgggggc agtgcaggat tttctggtgc tgtcagcaag cattcgacgg
       61 aacggaaaat cgctggcgca gatcgcgttt cacgacatcg gcccgatcgc gggtgtgtcg
      121 gcgatgatcg ctatcctgtt catcctcgtc atcgcgctgg cggggcttgg caaagtggtg
      181 gtgaaagcat taggcggtga accggcgtat cacgtccccg gcagctcatg gggaacgttt
      241 accatcgccg cgacgatccc catcgcgctg ttcattggcc tttacatgaa ccgccttcgc
      301 cgcgggcggg tgctggaggc ttcgatcgtc ggcgggttgt tgacgatcgg cgcgacggtg
      361 ctgggcggat gggtggcgca gcggccgatc ggtcattatt tcaatctgac ggcagggcaa
      421 gtggtttggg cgatggcggt atacgggttt atcgcggcgg tgctgccggt gtgggtgttg
      481 ctcgtgccgc gggattatct gagcagcttc ctcaagatcg gcacgatcgt gctgctggtg
      541 ggcggtgtca tcgtcgccaa cccgacgctg cgtgccccgg cgatcaattc agttttcacc
      601 agcggtggtc cggtggtgaa ggggaagatt tttccgttcc tgttcatcac gatcatgtgc
      661 ggggcgatca gcgggtttca cgcgctggtc agcagcggca cgacgccgaa gatggtcaag
      721 cgcgaatccg acgcccgcat gatcggatac ggcgcgatgc tgatggaagg actggtcggt
      781 gtggtggcga tgatcgccgc ggcgacattg ccggtggggg actattacgc gatgaacacc
      841 gagatcgccg cgatgccggc gtttcacgat cagatcataa agatccccgg cggtggcatc
      901 gagcatgtgg cggactacga agtgctgacg caggaatcgc tgcgcggccg gactggtggc
      961 gcggtcacgc tggcggtggg aatggcggag gtgttcgacc attcactagg cacgctggcc
     1021 ggcggggcga ggtggctttg gaagtattgg taccacttcg caatcatgtt cgaggcgctg
     1081 ttcatcctga ccacgatcga cgcggggacg cgcatcgggc ggttcttact ccaggagatg
     1141 ctcgggaaaa ttcatcccaa actgggtcag acgaactggt ggcccagcgc ctacctgagt
     1201 acggcgatca tcgtcggcgg ctgggcgtac ttcattaatg ccgactcgtt cgccgccatc
     1261 tgggcaatgt ttggcgtggc gaatcagatg ctcgcggtga tcgcgctggc ggtcgtgtcg
     1321 gtctacctgg tgaacgaagg ccgggcgaaa tacctgtggg tgacggtcgt cccgctggcg
     1381 gtggtgatga cgacgaccag caccgcggcg gtgcagatcc tgatcggcaa ggtggacggc
     1441 atccgcacgc agatagcgaa ggacgtgctg acggcggcgg agcgggcgct gctgatgaat
     1501 agcgcgatca ccgcagcgct gctggtggcg atgatggcgt gcacggtcct gatcatcgcg
     1561 tcggctgggg tgcgcatcgc cggcgttcgc cggcc
//
LOCUS       DATIMH010000857         1998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_202443, whole genome shotgun sequence.
ACCESSION   DATIMH010000857 DATIMH010000000
VERSION     DATIMH010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202443"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VLI90_14850"
     CDS             complement(<1..847)
                     /locus_tag="VLI90_14850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSV15537.1"
                     /translation="MTAESLFNQALSHAEPAQRSAFLDSACRGDAQLRASVDALLAAH
                     ERAGAVLQRTPVGIAETMQPTEEPWPNVAVQLSGGGEEPGSVVGRYKLLQRIGEGGCG
                     VVYMAEQEQPVRRRVALKVIKLGMDTRQVVARFEAERQALAMMDHPNIARVLDAGATE
                     TGRPFFVMELVRGVPITEYCDKNNLPTQRRLELFISVCQAIQHAHQKGIIHRDIKPTN
                     ILLTLHDGVPVPKVIDFGIAKATDQRLTDKTLFTAFEQFIGTPAYMSPEQAEMSGMDV
                     DTRSDI"
     gene            complement(968..1888)
                     /locus_tag="VLI90_14855"
     CDS             complement(968..1888)
                     /locus_tag="VLI90_14855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV15538.1"
                     /translation="MISLVVPIFNEAENLPEFRRRATAALDASGDAWEIVFVNDGSID
                     SSAALLRQYHADDPRLKLIELSRNFGHQPAVTAGVHHASGDCVILIDGDLQDPPEVIP
                     QLVAKWKEGFPVVLAVRRSRAERGVRRAGFALFYPIMNWMSDLPNGPDAGIFGLMDRT
                     VVAEFNKLPERNRFIPGLRSWLGFKQATVPYDRVDRAKGKPKQTLGRLLNYAMDGMVS
                     FSYKPLRAATYLGFIVSLFAFALAIFYIVTFYSFHKQITGFTTTITCVLFLGGVQLIC
                     VGILGEYIGRIYEEVKQRPLYVVQEKLGVE"
BASE COUNT          362 a          643 c          626 g          367 t
ORIGIN      
        1 agatgtcgct gcgcgtatcg acatccatgc cgctcatctc ggcctgctcg ggactcatgt
       61 aagccggtgt gccgatgaac tgctcgaacg cggtgaacag cgtcttgtcg gtgagtcttt
      121 gatccgtcgc cttggcgatg ccgaaatcga tcaccttcgg caccggcaca ccatcgtgca
      181 gcgtgaggag gatgttggtc ggcttgatgt cacgatgaat gatccccttc tgatgcgcgt
      241 gctgaatcgc ctggcagacg ctgatgaaca gctcgagccg gcgctgcgtc ggcaaattat
      301 ttttatcgca gtactcggtg atcggcacgc cgcggaccaa ctccatcacg aagaacggcc
      361 ggccggtttc ggtcgccccc gcgtccagca cgcgggcaat gttgggatga tccatcatcg
      421 ccagcgcctg ccgctcggct tcaaatcgcg cgacgacctg ccgcgtgtcc atgcccagct
      481 tgatcacctt cagcgcgacg cggcggcgca cgggctgttc ctgctcggcc atatagacga
      541 cgccgcaccc accttcaccg atgcgctgca gcagcttgta acgaccgacg accgaccccg
      601 gttcctcccc gccgccggac agttgcaccg cgacgttcgg ccacggttcc tcggtcggtt
      661 gcatggtctc ggcgatgccg accggcgtgc gctgcagcac cgcgcccgcg cgctcgtgcg
      721 ccgcaagcag cgcatcgacc gatgcgcgca gctgagcgtc cccgcgacag gcggagtcga
      781 gaaatgcgga gcgctgcgcc ggctccgcgt gagacagcgc ttggttaaac agcgattcag
      841 ccgtcatgct cgctcgcgcg ctccgttttg acaccaatgg cagcgtgccc attcatcata
      901 atcgcaagcg gcggaattgt tgatgattgc tcaatcacgt gaaggagccg cgttcgcgcg
      961 caggcactca ctccacgccc agcttctcct gcacgacata cagcggccgc tgttttactt
     1021 cttcgtagat ccggccgatg tattcgccca ggatgccgac gcagatcaac tgcacgccgc
     1081 cgagaaacag cacacaggtg atcgtcgtcg tgaagccggt gatctgtttg tggaacgagt
     1141 agaacgtgac gatgtaaaag atcgctagcg cgaacgcgaa cagcgacacg atgaagccca
     1201 ggtaggttgc cgcgcgcagc ggcttgtagc tgaagctgac catgccgtcc atcgcgtaat
     1261 tcagcagccg gccgagcgtt tgcttgggct tacccttggc gcgatcgact cggtcgtacg
     1321 gcaccgttgc ctgcttgaac ccgagccacg accgaagtcc cgggatgaac cggttgcgct
     1381 cgggtaactt gttgaactcg gcgacgaccg tgcgatccat caacccgaag atccccgcgt
     1441 cgggaccgtt gggcaggtcg ctcatccagt tcatgatcgg gtagaagagc gcgaagcccg
     1501 cgcggcggac gccgcgctcg gcgcgagatc gccgcacggc cagcacgacc gggaaacctt
     1561 ccttccattt cgcgaccagc tgcggaatga cttccggtgg gtcctgcaga tcgccgtcga
     1621 tcaggatcac gcaatcgccc gacgcatggt gcacccccgc ggtgacggcg ggctggtggc
     1681 cgaagttgcg ggagagttcg atcagtttca ggcgagggtc atcggcgtgg tattggcgca
     1741 gcaatgcggc ggaggagtcg atgctgccgt cgttgacgaa gacgatctcc cacgcgtcgc
     1801 cgctcgcgtc gagcgcggcc gtcgcgcgcc ggcggaactc cggcaggttc tcggcttcgt
     1861 tgaagatggg gacgacgagg gagatcatat tcaatgcgcg acaacgctag gcgtttagcc
     1921 gccgcggcct cgcggcgcgg atttcaaaaa cgcagcgcct tcttaagtcc gcgccgcgag
     1981 gccgcggcgg ctaaacgc
//
LOCUS       DATIMH010000858         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3092455, whole genome shotgun sequence.
ACCESSION   DATIMH010000858 DATIMH010000000
VERSION     DATIMH010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3092455"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1540)
                     /locus_tag="VLI90_14860"
     CDS             complement(155..1540)
                     /locus_tag="VLI90_14860"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HSV15539.1"
                     /translation="MLDQVLATIDQRRDASLAQLKEFLAIPSVSTKPDHAPHMLRCAT
                     WLADHLKFAGFNVSIEPTNGHPIVLAKNRHEPNRPTVLVYGHYDVQPPEPLDLWTTPP
                     FEPTVRKTEAGTDAVYARGAVDDKGQVWAHVEALTAWQANGGVPVNITLLIEGEEEIG
                     SENLEAFVKARKDDLQADIAVISDTNQFARGLPAITYGLRGLCYCEVFLTGPDHDLHS
                     GLYGGAVPNPANVLCEFIATLHDQDGRVNLPGYYDDVRALTETERDAWRKLPFTDASF
                     AKSVGIPQGSGEAGYSALERKWARPTCDVNGITTGYQGPGAKTVIPSKASAKISMRLV
                     PDQDPAKVQAALEQALRDRLPKNVRHEIKWYGASAPVLVPIDSPPTKLALEALRIGFG
                     TEPTFMREGGSIPVVGLIKSVLGLDTLLIGFGLPDDRVHSPNEKFDLDALHGGTRTAA
                     ALYAKLATRSK"
     gene            complement(1617..2111)
                     /locus_tag="VLI90_14865"
     CDS             complement(1617..2111)
                     /locus_tag="VLI90_14865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSV15540.1"
                     /translation="MPTLELPHTLGCLVCSRDNVHGLHLSLFVDPDASIVTTDFTPQP
                     ANIGFQGIVHGGVIATVMDEAMVWAATWVGRRFCLCGELTTRFRRPAEIGRRAIVEAK
                     VEFRNPRLIQTAATLRDESNQLLATASGKYVPVSLDQHVAFLATLVDEPATTTAANLL
                     RTAR"
     gene            complement(2207..3781)
                     /locus_tag="VLI90_14870"
     CDS             complement(2207..3781)
                     /locus_tag="VLI90_14870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV15541.1"
                     /translation="MPMWELFFKHAVTITYMVVLCTVALYGFHRYVLVYLYLKHKHDG
                     YKPARGFDELPRVTVQLPMYNEDVVAERIIKASCRIDYPKDKLQIQVLDDSTDHSADI
                     ARKACAEMAAQGYPISYIHRDNRVGYKAGALAAGLKSATGDYIAIFDADFIPPTDIFY
                     NVVDYFTDDKIGMVQIRWDHLNRDASLLTKSQAIFLDGHFVIEHTARNRSGRFMHFNG
                     TAGVWRRTTIDDAGGWQHDTLTEDLDLSYRAQLKGWQFVYLPQFCAPAELPPEMIGFK
                     QQAHRWTKGSMQTCLKLLPRILRSKHLPLHVKTEAFFHLTNTIVYPLMVVLTLLMYPT
                     FFNVYGPFKYYPKAQWLFGASLFILATCSASTFFAFAQQELFGKKAGWKSILYLPFLM
                     ALGVGISLNNAKAVIEAIWGHIKRKPSEFIRTPKYGVTGQDKNGWHAESVFTLKRLSL
                     PIIEIAFGSYMAACLFISIFYQFGRMSAPFLAIFAGGYFYVGVNSLYALYKMSCEAEE
                     TGDIDAAEASVDLPSV"
BASE COUNT          741 a         1172 c         1439 g          806 t
ORIGIN      
        1 tgactggggt gaatgggaag aaaatcgaat gaagcgcaac atgcgccgct gattgtgagc
       61 cagacacatc catatatacg ctgcaccaga agagacttac gcacgtcttg agctgcgcaa
      121 catgcgaaaa gtttttcttg cgcgctggct cgacctactt cgatcgcgtc gcgagcttgg
      181 cataaagcgc cgctgccgtg cgggttccgc cgtgcagcgc gtccaagtcg aatttttcgt
      241 tgggcgaatg cacgcgatca tccggcaggc cgaagccgat cagcagcgtg tccaggccca
      301 ggacggattt gatcaggcca accacgggaa tgctgccgcc ttcgcgcatg aaggtgggct
      361 cggtgccgaa gccgatgcgg agcgcttcga gcgccagctt cgttggcggg ctgtcgatcg
      421 gcacgagcac gggggcgctg gcgccatacc acttgatctc gtgcctcacg ttcttgggca
      481 gccgatcgcg cagcgcttgt tcgagcgcgg cttggacttt cgccgggtcc tggtcgggca
      541 cgaggcgcat ggagattttc gccgacgcct ttgaggggat gacggtcttg gcgccggggc
      601 cttggtagcc ggtggtgatg ccgttgacgt cgcaggtggg ccgggcccac ttgcgttcga
      661 gggcgctgta gccggcttcc ccgctgcctt gcgggatgcc gacacttttg gcgaagctgg
      721 cgtcggtgaa cggcagcttt cgccaggcgt cacgctcggt ctcggtgagc gcgcggacgt
      781 cgtcgtagta accgggcaag ttcacgcggc cgtcttggtc gtgcagggtg gcgatgaatt
      841 cgcatagcac gttcgccggg ttcggcaccg cgccgccgta taggccgctg tgcaggtcgt
      901 gatcggggcc ggtgaggaag acttcgcagt agcacaggcc gcgcaggccg taggtgatgg
      961 cgggcaggcc gcgggcgaac tggttggtgt cgctgatgac ggcgatgtcg gcttgcagat
     1021 cgtccttgcg cgctttgacg aatgcttcga gattttcgct gccgatttcc tcttcgcctt
     1081 cgatgagcag cgtgatgttg accggcacgc cgccgttggc ttgccaggcg gtgagcgctt
     1141 cgacgtgggc ccagacctgg cccttgtcat cgaccgcgcc gcgcgcgtac acggcgtcgg
     1201 tgccggcttc ggtcttgcgc acagtcggtt cgaacggcgg cgtcgtccac aggtccagcg
     1261 gctcgggcgg ttgcacgtcg tagtggccgt agacgaggac cgttgggcgg tttggttcgt
     1321 ggcggttctt ggccaggacg atcgggtgac cgttggtcgg ttcgatgctc acgttgaagc
     1381 cggcgaactt cagatgatcg gcaagccagg tggcgcagcg cagcatgtgc ggggcgtgat
     1441 cgggcttggt gctgacgctg gggatggcga ggaactcttt gagctgggcg agggaggcgt
     1501 cgcggcgctg gtcgatggtg gcgaggactt ggtcgagcat gatgagtctt agtgtacgcg
     1561 gggggagctt gccgctgaag cggcaagccc ggtcggcaag ggggcgcttc tgcgcgttat
     1621 cgagcggtgc ggaggaggtt cgccgcggtg gtggttgccg gttcatccac gagcgtcgcg
     1681 agaaaagcga cgtgctgatc gagcgagacc ggcacatatt tcccgctcgc ggtcgcgagc
     1741 agttggttcg actcatcgcg cagcgtggcg gcggtctgaa tcaggcgggg attgcggaat
     1801 tcgaccttcg cttcgacgat cgcgcgccgc ccgatctccg ccgggcggcg gaagcgtgtc
     1861 gtcagttcgc cgcagaggca aaagcgtcgg cccacccagg tggcggccca gaccattgct
     1921 tcgtccatca cggtcgcgat cacaccgccg tggacgatgc cttggaagcc gatgttcgcc
     1981 ggctgcggcg tgaagtcggt ggtgacgatg ctggcatcgg gatcgacgaa gagcgacagg
     2041 tgcaggccgt gcacgttatc ccgagagcaa acgaggcagc cgagcgtgtg gggcagttcg
     2101 agcgtgggca tggtggggaa ttgaacgcag ccgcttgcgg cttggcagac catgccgcct
     2161 ggtttgcgaa gccgcaagcg gctgggtgcg cgcgatcgtg agatcatcac accgacggca
     2221 ggtccaccga cgcttcggcg gcgtcgatgt cgccggtttc ttcggcttcg cagctcatct
     2281 tgtacagggc gtacagcgag ttgacgccga cgtaaaaata cccgccggcg aagattgcca
     2341 ggaacggggc gctcattcgg ccgaactggt agaagatgct gatgaacagg cacgccgcca
     2401 tgtaggagcc gaaggcgatc tcgatgatcg gcaggctcaa tcgcttgagc gtgaagacgc
     2461 tctcggcgtg ccagccgttc ttgtcctggc cggtgacgcc gtacttcggc gtgcggatga
     2521 actcactggg ctttcgcttg atgtggcccc agatggcttc gatgacggcc ttggcgttgt
     2581 tcaagctgat gcccacgccc agcgccatca ggaacggcag gtacaggatg gatttccaac
     2641 cggccttctt gccgaacagt tcctgctgag cgaacgcgaa gaacgtgctg gcggagcatg
     2701 tggcgaggat gaacaggctg gcgccgaaga gccactgcgc tttgggataa tatttgaacg
     2761 gaccgtagac gttgaagaac gtcgggtaca tcagcagcgt gaggacgacc atcaacgggt
     2821 agacgatcgt gttggtcagg tgaaagaacg cctcggtctt cacgtgcagc ggcaggtgct
     2881 tggatcgcag gatgcgcggc agcagcttca ggcacgtctg catcgaaccc ttggtccagc
     2941 gatgcgcctg ctgtttgaac ccgatcattt ccgggggcag ctcggctggc gcgcagaact
     3001 gcggcaggta gacgaactgc caacccttca gctgagcgcg ataggacaga tcaagatctt
     3061 cggtcagcgt atcgtgctgc cagccccccg cgtcgtcgat ggtcgtgcga cgccagacac
     3121 cggcggtgcc gttgaagtgc atgaaccgcc cgctgcgatt tcgcgcggtg tgctcgatca
     3181 cgaagtgacc gtccaggaaa atcgcctggc tcttggtcag cagcgatgcg tcgcggttca
     3241 ggtgatccca gcggatctgc accatgccga tcttgtcgtc cgtgaagtag tccacgacgt
     3301 tgtaaaagat gtcggtgggc ggaatgaagt cggcgtcgaa gatggcgatg tagtcgcccg
     3361 tcgcgctttt caatccggcc gccagcgcgc cggccttgta accgacgcgg ttgtcgcgat
     3421 ggatgtacga gatggggtaa ccctgggcgg ccatctccgc gcacgctttt cgcgcgatgt
     3481 cagccgaatg atccgtgctg tcgtccagca cctggatctg cagtttgtcc ttcgggtagt
     3541 cgatccggca ggacgccttg atgatgcgct cggcgacgac gtcctcgttg tacatcggca
     3601 attgcacggt cacgcgcggt agctcgtcga acccgcgcgc gggcttgtat ccatcgtgct
     3661 tgtgcttcag gtacaggtag accaggacgt agcggtggaa gccgtacagc gcaaccgtgc
     3721 acaggactac catgtaggtg atcgtgacgg cgtgtttaaa gaacagctcc cacataggca
     3781 ttggtctcgg cgaaaagggc gcgtgcggac cgccggttga gcaatcgatc gccgcgggca
     3841 gtcatggacg tcgctaacaa aaccaaaagt cccccaggca cgtgcggcgg aaaaaaaaga
     3901 cgcacgctcg gcaacaggcc tcgggaagcc gtgccaacgc gagcaagggg cggcgcgatg
     3961 caggcgggct gatcttaagg aaagccccac ataactgtca acaatcgccg ttcagaacgt
     4021 aacttagagc ttctaccgcc gccacttaac gcgttcgcaa catgcctgcg aaacagacta
     4081 tccgcgtgcg gtgggattgt tgcagagatc acgcatttgc ggaagtaggg aattccgccg
     4141 ggccgtgcgc ggtgggaa
//
LOCUS       DATIMH010000859         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1650558, whole genome shotgun sequence.
ACCESSION   DATIMH010000859 DATIMH010000000
VERSION     DATIMH010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650558"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VLI90_14875"
     CDS             complement(<1..331)
                     /locus_tag="VLI90_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15542.1"
                     /translation="MTANAPIVARSFVPAQTDPTQFEQIEPLYKQLLDRPLETRAEIE
                     RFLADFSELSAVVEEQGTRLYIDKSCHTDDAEIERRYLHFVESVEPRTKPLYFQLQKK
                     LLESPAVL"
     gene            414..1196
                     /locus_tag="VLI90_14880"
     CDS             414..1196
                     /locus_tag="VLI90_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSV15543.1"
                     /translation="MTVTRGGRKILRDITWRVPAGACAAILGPNGSGKSTLARVIMGQ
                     MWPAAGEVAVLGERFGDTDLNALRASIRLVQSNGVVEFDADETTLNVVLTGFFGTVGL
                     YDPVTPAMRRSAIRLIRQVGLTKEAQQPYCTLSSGERMRCLIARALVVKPRLLILDEP
                     TAGLDLLARERVLATVQRLVDSDRQPPTVLMITHHVEELLPATASVLILKDGRAAAAG
                     KPRAVLTSAVLSKVYQFPVEVKRRAGRFWVQVHPSAWGGLVR"
     gene            complement(1263..>1742)
                     /locus_tag="VLI90_14885"
     CDS             complement(1263..>1742)
                     /locus_tag="VLI90_14885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSV15544.1"
                     /translation="LLFDIDNFKKYNDQYGHGVGDDILRQTAALMKRCVRDHDLVARI
                     SGDEFAVVFWEKEGPRQPRDPQAGAPGRPPQAPQQILERFRRLLATQNFPGLGPGGQG
                     TLTISGGLAVFPYDAQTVNDLIEAADRALMFKAKQSGKNSIFLVGGKAPGDLGNAQT"
BASE COUNT          291 a          516 c          599 g          336 t
ORIGIN      
        1 gtaaaaccgc tggcgattcc agcagcttct tctgcagctg aaaatagagc ggcttggtcc
       61 gcggttcgac cgattcgacg aaatgcaggt aacgccgttc gatctcggcg tcgtcggtgt
      121 ggcagctctt gtcgatgtac agccgggtgc cctgttcttc gacaaccgcc gacaactcag
      181 agaaatcggc aagaaaccgc tcgatctctg cccgcgtttc cagtgggcga tcgagcaact
      241 gcttgtagag cggctcaatc tgctcgaact gcgtgggatc ggtttgcgcg gggacaaatg
      301 aacgggcaac gatcggcgcg tttgcggtca tcagtggttc ctttaatctc ttgcgcgcgg
      361 aggctacgtc aaagatgaaa gccgggcaac caaccgcgat tgacctacag cgcgtgaccg
      421 tgacacgcgg cgggcggaag attttgcgcg acatcacctg gcgggtgccg gccggggcgt
      481 gcgcggcgat ccttggtccc aacggcagcg gcaagagcac gctggcccgc gtgatcatgg
      541 gccagatgtg gccggccgct ggtgaggtcg cggtgctcgg cgagcgtttt ggcgacacgg
      601 atctgaacgc gctgcgcgca tccatccgcc tcgtgcagtc gaatggcgtc gtcgaatttg
      661 acgcggacga gacgacgctg aacgtcgtcc tcaccggctt cttcggcacc gtcgggttgt
      721 acgatccggt cacgccagcg atgcgacgat ccgcgattcg cttgatccgc caggtcgggc
      781 tgaccaagga agcgcagcag ccatattgca cgctcagcag cggcgagcgg atgcgctgcc
      841 tgatcgcgcg ggcgctggtc gtgaagccgc ggctactgat tttagatgaa ccgacggcgg
      901 ggctcgatct gctggcgcgc gagcgcgtgc tggcgacggt gcagcggctg gtggacagcg
      961 atcgccaacc accgacggtg ctgatgatca cgcatcacgt cgaagagttg ctgccggcga
     1021 cggcgtcggt gctgatcttg aaagacggcc gagccgcggc ggccgggaag ccgcgggcgg
     1081 tgctgacgtc ggcggtgttg tcgaaggtgt atcagtttcc agttgaggtg aagcggcgcg
     1141 ccgggcgatt ttgggtgcag gtacacccgt cggcgtgggg tggtttggtg cggtagccgc
     1201 ttgcggctta gcatctgttt tccccccgcg tgaagaaaaa ggatgctaag ctgcaagcgg
     1261 catcacgtct gcgcattccc gagatccccg ggcgcttttc cgccgacgag gaagatgctg
     1321 ttcttgcccg attgcttcgc cttgaacatc agcgcccggt cggcggcttc gatcaggtcg
     1381 ttcacggttt gcgcgtcgta cggaaagacc gcgaggccac cgctgatcgt gagcgtgcct
     1441 tggccaccgg ggcccaggcc ggggaagttc tgcgttgcca gcaaccggcg gaagcgttcg
     1501 aggatctgct gcggcgcctg cggcggccgg ccaggcgcgc ccgcctgcgg gtcgcgcggt
     1561 tggcgcgggc cttccttctc ccagaagacc accgcgaatt cgtcgccgct gatgcgcgcg
     1621 accaaatcgt gatcgcgcac gcagcgcttc atcagcgcgg cagtctggcg caggatgtca
     1681 tcgcccacgc cgtggccgta ctggtcgttg tattttttga agttgtcgat gtcgaacagc
     1741 ag
//
LOCUS       DATIMH010000860         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4829229, whole genome shotgun sequence.
ACCESSION   DATIMH010000860 DATIMH010000000
VERSION     DATIMH010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4829229"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VLI90_14890"
     CDS             <1..882
                     /locus_tag="VLI90_14890"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HSV15545.1"
                     /translation="GFFSQNGNGDMTGTHWLEESGFLDGPVLITNTHSVGVVRDAFLA
                     WLVNNKRRPGTNTFDGGFYTYPVVAETWDGFLNDINGFHVKPEHVFRALESASGESVA
                     EGNVGGGTGMVCYGFKGGIGTSSRTAGPYKVGVLVQCNCGSRRQLRIGGMPVGAKLAE
                     ALPPLASAGHPYREDTGSIIIVVATDAPLLPHQTKRLARRATMGLARTGSVSGNGSGD
                     IFIAFSTANAKVAAAKSAVNVSMLPNDEINAVFEAAVQATEEAIVNAMVGAETMRGNE
                     GRTVYALPHDRVRAMFP"
     gene            complement(930..>1641)
                     /locus_tag="VLI90_14895"
     CDS             complement(930..>1641)
                     /locus_tag="VLI90_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15546.1"
                     /translation="HQVGSLPPDPCHGILMFHDAAGHELLRGAYDLKPGETRSLRYTL
                     PVRDLDISVRVPIDPCWLPSPGGRAVGSVEVFDSLGKVTHFANPAATRMTDFNDGSKG
                     RPDPGALVGFNPQPDPPAFGMVSLKNQAIRMNVSCFEHPVNGVPPGPCRGTAMFHNAA
                     GDVVKSGRYDLKAGETAALLYDPAAAAGSTVAIIPCVLPEIGGRAVPNVEVIDTATGD
                     TALLINPAADRISDFQRQ"
BASE COUNT          304 a          518 c          528 g          291 t
ORIGIN      
        1 ggatttttct cgcagaacgg gaacggcgat atgaccggca cgcattggct ggaagagtcg
       61 ggctttctcg acgggcccgt gctcatcacc aacacgcata gcgtcggcgt cgtgcgcgat
      121 gcgttcctcg cgtggctcgt caacaacaag cgccggcccg gaaccaacac gttcgatggc
      181 ggcttctaca cctacccggt ggtcgccgaa acctgggacg gctttctcaa tgacatcaat
      241 ggctttcacg tgaagcccga gcacgtcttc cgcgccctgg aatccgcgtc gggcgagtcg
      301 gttgcggagg gcaacgtcgg aggcggcaca ggaatggtgt gctacggatt caagggcggc
      361 atcggcacgt catcccgcac ggcgggaccg tataaggtcg gcgttttggt gcaatgcaac
      421 tgcggcagcc gacggcagtt gcggattggc gggatgcccg taggcgcgaa actcgcggaa
      481 gccctgcctc cgctcgccag cgccggacat ccttatcgcg aagacaccgg ctcgatcatc
      541 atcgtggttg cgacggatgc tccgcttctc ccgcaccaga caaaacgtct cgcacggcgc
      601 gcgacgatgg gcctggcgcg cacaggctca gtctcaggta acggctccgg cgacattttc
      661 atcgcgtttt cgaccgccaa tgcgaaggtc gccgccgcca aatcggctgt gaacgtgtcc
      721 atgctcccga acgacgagat caacgccgtg ttcgaggccg cggtgcaagc taccgaggaa
      781 gcgatcgtca acgcgatggt gggcgcggaa acgatgaggg gaaacgaggg ccggactgtg
      841 tacgccctgc cacacgaccg cgtccgcgcg atgttcccgt aagcggcgcc atcccggtca
      901 ggcggtccac gcccgctgca tcgccggctt tactgccttt ggaagtcact aatccggtcc
      961 gccgccggat tgatcaacag cgcggtatcg cccgtggccg tatcgatgac ctcgacgttc
     1021 ggaacggcgc ggccaccgat ctccggcaga acgcacggga tgattgccac ggtgctgccc
     1081 gccgccgccg ccgggtcata aaggagggcc gccgtttcac cagcctttaa gtcatagcgt
     1141 ccgctcttca ccacgtcgcc tgctgcattg tggaacatag ccgtgccgcg gcaggggccc
     1201 ggcggaacgc cgttcacggg atgttcgaag cacgagacgt tcatgcggat ggcctggttc
     1261 ttcaggctca ccattccgaa tgcgggaggg tcgggctgcg ggttgaagcc gaccagggct
     1321 ccggggtctg ggcggccctt gctgccatcg ttaaaatcgg tcatccgtgt ggccgcaggg
     1381 ttggcgaaat gcgtgacctt gccgaggctg tcgaacacct cgaccgaacc taccgcgcga
     1441 ccgccgggac tcggcaacca acacggatcg atgggcacgc gaacgctgat atccaaatcg
     1501 cgcactggga gcgtatagcg aagcgacctg gtctcgccgg gtttcagatc gtatgcgcct
     1561 ctcaacagct catgtcctgc cgcgtcgtgg aacatgagga ttccgtgaca ggggtccggc
     1621 ggtaaagagc cgacctgatg a
//
LOCUS       DATIMH010000861         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3671144, whole genome shotgun sequence.
ACCESSION   DATIMH010000861 DATIMH010000000
VERSION     DATIMH010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671144"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VLI90_14900"
     CDS             complement(<1..1339)
                     /locus_tag="VLI90_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HSV15547.1"
                     /translation="MILNTRRLLVIGLILFIPAALTALGATPSDASADGGGRTYNLTD
                     YGGVGDGNTINTDAFRKAIETCAANGGGRVRVPAGTFVTGPIQLMSGVDMHVDRDAVV
                     LFSRKLDDYPLVKANWEGRPTVMCQAPISGDNLHDVSFTGPGTFDGSGDAWRMVKQDK
                     LTSEQWDKLVKSGGYVDVKKKTWYPSEAVPKLAPALKKLRDAAGEPNTDDYTKYRDLL
                     RPELVRITNSNHVTLDGPTFRNSPSWNIHVLYCENVAVRNVTLFNEAYAQNGDGIDVD
                     ACRNVRITDSAIYAGDDDICLKSGRDEAGRRAGRATENVTVDNCTIFWGHGGLTIGSE
                     MSGGVRNVNVTNCIMRGTDIGLRFKTTRGRGGVVENVNVANIAMHDIKGTAILFDMYY
                     QVKGKPTTEPVSERTPIFRQFAINNVTCQGAKTALEIRGLPELAISDITLEKVR"
     gene            1580..3010
                     /locus_tag="VLI90_14905"
     CDS             1580..3010
                     /locus_tag="VLI90_14905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSV15548.1"
                     /translation="MHHSIFRTILSTAALGPLLLAGCSAYHEAPLDPAAVEQHLATPT
                     DRQLQAQADEIKHPLLKPIQLDPRDGMSPDEAAILAVILNPTLRADRNRRDLAHAELI
                     RAGILPNPQLSYNYDVVTGGNTEGAVNAWGLGLSWDVTSLISHETKLNAAKAGAAAVN
                     LDIAWQEWQIAEAARTAVYDLLALQGQLAVAQDVDRRLQENLNVVGKAVDEHLKTALD
                     LAAAEAAARDSHALVLQNQRDIDHQRLQLNRAIGLPPGTRVKLTPGIVLPSRFDPPPL
                     AELIRDLEERRLDLLGLKRGYQSQDQTLRAAILDQFPRINIGFNRANDNTNVHSLGPA
                     VTIDLPIFDRNQANIAAESATRKKLLDEYIARVYDARADIASAIDDIRSINEQIKDAE
                     AAVPNLQHLVDTYREATDRGNADVLSYYEAWNNLARKNLDVLKLKQQLADNRIALEIA
                     AGRYCPDTAENPATQPTALAESRTNP"
     gene            3007..>3649
                     /locus_tag="VLI90_14910"
     CDS             3007..>3649
                     /locus_tag="VLI90_14910"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSV15549.1"
                     /translation="MKPRTVAIAKIAIAFALICASDFTRRASAGDEDEAKGTAAVKTA
                     VIRFGAITQSITAFGSVTAEPGAVTVFSVPFESRVKHILISAGQQIDKDVALIEVEPS
                     PEAKLALLEARNAVEAANKDLEQTQQRFNLKLATNTELLTSQQAAQVAKMRLESLQQR
                     GEAEDSRKINADGPGLVSKVDVQEGQIVPAGGPLVETTPRTSIEVRLGVEPPEI"
BASE COUNT          693 a         1220 c         1108 g          628 t
ORIGIN      
        1 ttcgcacctt ctccagcgtg atgtcggaaa tcgccagctc cggcagcccg cgaatttcca
       61 gcgccgtctt cgccccctgg cacgtcacgt tgttgatcgc gaactgccgg aagatcggcg
      121 tgcgctcgct caccggctcg gtcgtcggct tgcccttcac ctggtaatac atgtcgaaca
      181 ggatggcggt gcccttgatg tcgtgcatgg cgatgttggc gacattcaca ttctcgacca
      241 caccaccgcg gccgcgcgtc gtcttgaagc gcaggccgat gtccgtgccg cgcatgatgc
      301 agttggtgac gttcacgttg cgcacgccgc cggacatttc gctgccgatc gtcaaaccgc
      361 cgtggcccca gaagatcgtg cagttgtcca cggtgacgtt ttccgtcgcg cgccccgcgc
      421 gccggcccgc ttcatcgcgg ccggacttca ggcagatatc gtcatcgccg gcgtaaatcg
      481 ccgagtctgt gatgcgcacg tttcggcacg cgtccacgtc gatgccatcg ccgttctgcg
      541 catacgcttc gttgaacagc gtgacgtttc gcaccgcgac gttctcgcaa tacagcacgt
      601 ggatgttcca gctgggcgag ttgcgaaacg tcgggccgtc gagcgtgacg tgattcgaat
      661 tggtgatgcg caccagctcc ggccgcagca ggtcgcggta cttggtgtag tcgtcggtgt
      721 tcggctcacc cgccgcgtcg cgcagcttct tcagcgctgg cgccagcttc ggcaccgctt
      781 cgctcgggta ccacgtcttt ttcttcacat cgacatagcc acctgatttg acgagcttgt
      841 cccactgctc ggacgtgagc ttgtcctgct tcaccattcg ccaggcgtcg ccgctgccgt
      901 cgaacgtgcc ggggccggtg aacgacacat cgtgcaaatt gtcgccggaa atcggcgcct
      961 ggcacatcac ggtcggccgg ccttcccagt tcgccttcac cagcgggtaa tcgtcgagct
     1021 ttcggctgaa cagcacgacc gcgtcgcgat caacgtgcat atcgacgccg ctcatcagct
     1081 ggatcggccc ggtgacaaac gtgcccgccg gcacgcgcac gcggccacca ccgttggcgg
     1141 cgcacgtctc gatcgccttg cgaaaggcgt cagtatttat cgtgttgccg tcgccgacac
     1201 cgccgtagtc ggtgaggttg taggtgcgac cgccaccgtc ggcactcgca tcgctcggcg
     1261 tcgcgccgag cgcagtgagt gccgctggaa tgaacagaat cagaccgatg acaagaagcc
     1321 gccgcgtgtt caggatcatg acctcgctcc ggttcacacg catcctaacg ccagactttt
     1381 gcgcgccgag gggcggcacg ccgcggatca aagattcggc ggaaagtgcc aagtgtgtcc
     1441 gagacgccag ctaccgctgc gcagcgtgtg gcatagggac gcgttcaact gctcccttgc
     1501 atgcgccgcc ccgacacatc atcttcttgt catgttgtcc ccttagtctt tcgccctcac
     1561 ggcggctgag gcattctgca tgcatcactc gatattccgg acgatcctct cgacagccgc
     1621 gctcgggccg ctgttgctgg ccggttgctc ggcctatcac gaagcgccgc tggaccccgc
     1681 ggccgttgaa caacatctcg ccacgccgac cgatcggcag ttgcaagcgc aggccgatga
     1741 gatcaagcac ccgctgctca agcctatcca gctcgatccg cgcgacggca tgtcgccgga
     1801 cgaggcggcc atccttgcgg tcattctcaa tccaacgctg cgtgccgatc gaaaccgtcg
     1861 cgatcttgcg catgcggaat tgatccgcgc cggaatcctg cccaacccgc agttgtcgta
     1921 caactacgac gtcgtcaccg gcgggaacac cgaaggggcg gttaacgctt gggggcttgg
     1981 actcagttgg gacgtcacgt cgctgatcag ccacgagacg aaattgaacg cggccaaggc
     2041 cggagcggcg gcggtgaatc tggacatcgc ctggcaggaa tggcaaatcg ccgaggccgc
     2101 gcgaaccgcc gtgtacgatc tgctggcgct gcaaggccag ctggcggtcg cgcaggacgt
     2161 cgatcggcga ttgcaggaaa acctgaatgt cgttggcaaa gccgtcgatg aacatctgaa
     2221 aaccgcgctc gaccttgcgg cggccgaggc cgcggcgcgt gactcgcacg cgcttgtttt
     2281 gcagaaccag cgcgacatcg accaccagcg gttgcagctg aaccgggcga tcggcctgcc
     2341 gccgggaacg cgcgtgaagc tgacgccggg gatagttctg ccgtcgcgat tcgatccacc
     2401 accgctggcc gagctgattc gcgacttgga agaacgccgg ctcgacctgc tgggtttgaa
     2461 gcgcggctac caaagccagg atcagaccct ccgcgccgcc atcctcgatc agttccccag
     2521 gatcaacatc ggcttcaacc gcgccaacga caacaccaac gtgcattcgc tcggcccggc
     2581 cgtcacgatc gatcttccga tcttcgaccg caaccaggcc aacatcgccg ccgaaagcgc
     2641 gacgcggaaa aagctgctcg acgaatacat cgcccgcgtg tacgacgccc gcgcggacat
     2701 cgcgtcagcg atcgacgaca tccgctcgat caacgagcaa atcaaagacg ccgaggcggc
     2761 cgtgccgaac cttcagcatc tggtggacac ctatcgcgaa gcgaccgatc gcggcaacgc
     2821 cgacgtcctt agttactatg aagcgtggaa caaccttgcg cggaagaacc tggacgtcct
     2881 gaagctgaag cagcagctgg cggacaaccg aatcgcgctg gagatcgccg ccggacgata
     2941 ttgccccgac acggctgaaa acccggcgac gcaaccgacc gcgctggccg agtcacggac
     3001 gaatccatga agccacgaac agtcgccatc gccaagatcg ccatcgcgtt cgcgctgatc
     3061 tgcgcgagtg atttcacccg tcgcgcatcg gcgggggacg aagacgaagc caagggcacc
     3121 gccgcggtga agacggcggt catccgcttt ggcgcgatca cccaaagcat cacagccttc
     3181 ggcagcgtga ccgctgaacc cggcgccgtc acggtcttct ccgttccgtt cgagtcgcgc
     3241 gtgaagcaca tcctgatcag cgcgggccag cagatcgaca aggatgtcgc gctgatcgag
     3301 gtcgagccca gccccgaagc gaagctcgcg ctgctcgaag cccgcaacgc cgtcgaggcg
     3361 gccaacaagg acctggaaca aacgcagcag cgcttcaacc tgaagctggc gacgaacacg
     3421 gaactgctca cttcgcagca agcggcgcag gtcgcgaaaa tgcggctgga gagcctccaa
     3481 cagcgcggcg aagccgagga ctcgcgcaag atcaacgccg acggcccggg cctggtcagc
     3541 aaggtagacg tgcaggaagg gcagatcgtg cccgccggcg ggccgctggt ggagacgacg
     3601 ccgcggacca gcatcgaagt tcgcctcggc gtcgagccgc cggagatct
//
LOCUS       DATIMH010000862         1798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3954432, whole genome shotgun sequence.
ACCESSION   DATIMH010000862 DATIMH010000000
VERSION     DATIMH010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3954432"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VLI90_14915"
     CDS             <1..981
                     /locus_tag="VLI90_14915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV15550.1"
                     /translation="MGGGTRNPMKLLVVIVNYRSADLAIDCLASLAPQVRNDMRIIIT
                     DNQSPDDSVAKLAAVIERNGWSAWATLRPLDRNGGFAYGNNRGIEPALAADDKPQYIL
                     LLNPDTVLRPGAVQTLIDFMDANPTVGIAGSRLEDPDGTPQVSAFRFHSIAGEIERSM
                     RLGVVTRLLSNAAIAPPVSNEAGPCDWVAGASMIIRREVFERIGLMDEKYFMYFEEVD
                     FCLQAKRVGWPCWYVPSSRVVHLVGAVSQMSDARKHRKRRPAYWFESRRRYFVKNHGW
                     LYAAMADLAFMVGFTLWRIRRLVQRKDDPDPPKLLLDSLAHSVFVRGRTV"
     gene            1170..1769
                     /locus_tag="VLI90_14920"
     CDS             1170..1769
                     /locus_tag="VLI90_14920"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HSV15551.1"
                     /translation="MTAPATTTPPVEPSRRASLAAGAASAPSPPIGLFSQIAEDWRAH
                     DRDWTRPGFRAVAAHRLGVWRMTVRPKLLRAPLSIIYRYLYRKCRNRYGIELPYSAKL
                     GRRVVIEHQGAIVIHGHSEIGDDTIIRQGVTLGNKTLDRPYDAPKLGARVNVGAGAKI
                     LGAVTVGDGATVGANAVVVKDVPPNAIAVGIPAMIVLGK"
BASE COUNT          320 a          631 c          557 g          290 t
ORIGIN      
        1 atgggcgggg ggaccagaaa tccgatgaag ctactggtcg tcatcgtcaa ctaccgctcc
       61 gccgacctgg ccatcgactg cctcgcgtcg ctcgcgccgc aggtgcgcaa cgacatgcgc
      121 atcatcatca ctgacaacca atcccccgat gactcggtcg ccaagctcgc cgccgtcatc
      181 gagcgcaacg gctggtccgc atgggcgacg ctgaggcccc tcgatcgcaa cggtgggttt
      241 gcctacggca acaaccgcgg catcgaaccg gcactggcgg cggacgacaa gccgcagtac
      301 atcctgctgc tgaaccccga caccgtgctt cgccccggcg ccgtgcaaac gctgatcgac
      361 ttcatggatg ccaaccccac cgtcggcatc gccgggtcgc gcctggaaga ccccgacggc
      421 accccgcagg tctcggcgtt tcggttccac tcgatcgcgg gtgaaatcga gcgctcgatg
      481 cgcctcggcg tcgtcacgcg gttgctgtcg aacgccgcca tcgccccgcc ggtttcaaat
      541 gaagctggcc cgtgcgactg ggtcgccggc gccagcatga tcatccggcg tgaagtgttc
      601 gagcgcatcg ggctgatgga tgaaaaatat ttcatgtact tcgaagaggt ggacttctgc
      661 ctgcaggcca agcgggtcgg ctggccgtgc tggtacgtgc cgagcagccg ggtggtgcac
      721 ctggtcggcg cggtgtcgca gatgagcgac gcccgcaagc accgcaaacg ccgcccggcg
      781 tattggttcg aatcgcgccg gcgatacttc gtaaaaaacc acggctggct ctacgccgcg
      841 atggccgacc tggcgttcat ggtcggcttc acgctgtggc gcatccgccg gctcgtgcag
      901 cgcaaggacg accccgatcc gccgaaattg ctgctggatt cgctggcgca cagcgtcttc
      961 gtccgcggcc gaacggtgta gagtagggcg ctcatcgtta cccccacgcc gggttcggag
     1021 gtactccgac gacccggatt cttggcctcg tagcgccgca gatccgggtc gtcggagtac
     1081 cctccgaacc cggcgtggtg aagcgcgaat agctcgcccc ttcacaatgc ggctaccgat
     1141 tttcgcgcgc tgctatagaa ttgatgagga tgaccgcacc cgccaccaca acgccgcccg
     1201 ttgaaccgtc gcgcagggcc tcgctcgccg ccggcgccgc ctcggcgccg tcaccgccga
     1261 tcgggctgtt ctcgcagatc gccgaagact ggcgcgctca cgaccgcgac tggacgcgcc
     1321 ccggctttcg cgccgtcgcc gcccaccgcc tcggcgtgtg gcgcatgacc gtccgcccga
     1381 agttgctgcg cgcgccgctg agcatcatct accgctacct atatcgcaaa tgtagaaatc
     1441 gctacggaat tgaactcccc tactcagcga agctgggccg gcgcgtcgtg atcgagcacc
     1501 aaggcgcgat cgtgatccac ggccacagcg aaatcggcga cgacacgatc atccgccaag
     1561 gcgtgacgct cggtaacaag acgctcgatc gcccgtacga cgccccgaaa ctcggcgccc
     1621 gcgtgaacgt cggcgccggc gcgaagatcc tcggcgccgt cacggtcggc gacggcgcga
     1681 ccgtcggcgc caacgcggtg gtcgtgaaag acgtcccgcc caacgccatc gccgtgggca
     1741 tcccggcgat gatcgtgctg ggaaaatgac gaaacccgaa gcccgaatcc ccgaatga
//
LOCUS       DATIMH010000863        14287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1072668, whole genome shotgun sequence.
ACCESSION   DATIMH010000863 DATIMH010000000
VERSION     DATIMH010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 14287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14287
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072668"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3446
                     /locus_tag="VLI90_14925"
     CDS             <1..3446
                     /locus_tag="VLI90_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSV15552.1"
                     /translation="TADFNGTPLVVPDNDSVLVVARGGKGNQFEDGYVVCLSLSGGQY
                     KWSTYVGSASHMFDMGDNGVEQLGTSEMCLAEGRVFVLTNLGTVAALDPYDGRMIWLS
                     AYPRDVTDNPEQMQMVFRNRMANGGRAGTNKPWANNPVIVANGKVFVLPTDGRHLLVY
                     DAGTGAEANRILLSDYDNADVLLGVRPRMAILSTEKGCYCIDWRAYTHDDAEHAIIWA
                     RKDVAADTVAGEENTIFGRGFVTSDSVFLPTRHRLYQLGWFKGGKVMSMYPSRGQWSG
                     DEEPGNVLATSQGVIIAGSTRVEVYRDLTLVRRQFEERIAASPNDPQPRIDYASALFA
                     SGQLDAAVAKLDEAIEMIGGQSAMRPGKGRDLVFTTALSFAERAAKDPANETGSVTEK
                     LYDRAAAAADSPLDQAVYRLSRARHLHDRKDYAGEVKLCQEVLSDDAMRAVSVNDNTT
                     AGAEAEAAVNGAMLADRTAYAPIEQAAAAAVKAASAADDPSQMLAVARVYPNSRAAAE
                     ARQAAAKLLEAAKRYDEAIATLREMYAGSLDPAVRTNLLVSVANDYLTMPGGVGPAID
                     RLAMATAKDQVQDRMPQALILPDGTRIENATFTAAIGTLREMQEKEDVARLPDFHIPV
                     QVHAKDKAFTRTEGSLVIPDVTALVRPLRDFPRNDRVVAWSATAGLSIYPVGESTPVT
                     TMPASALPESPGGCAWIDPAKLVLWSGSRVMLLGDGAGAGPKWQTPLPSGPALSTTVA
                     DSRGAIDDEQVAMDQAAGNAGAAGPNGIVIDGGFGGGGRIIVRNGRQFIINGGQIRFI
                     AQGNIGIAGQPAAAPAPAGAAAVGLGPQITMFRPAGDRIVLSTSLGTLMGLKVNDGGI
                     AWQMRLAEGPLTQVLANGRFTVARIDDPAGAQLIVLDTPTGRVIGRRRFGPDGTGNQL
                     MNVALSEEATLVWTTLAKLLSKDLYEPWRNPPVDLSTRPTGEAMNFAGLNQPDQLLVR
                     AGRVVALYDGGKFVRVQDLSNRRIDPVTPAANPLATGADTTAGSASVALRLVGPRLFI
                     TNGRNLWQYNLASADDHYKTLLFDLDYPPRIRELFLGQDHAILLNEPVDRGPLGSSFV
                     QLLIHRRGPIGPGNAREASNLDYYPQITDKSGIFAWQAVDGGMYYLSGDHQLHFLQAR
                     K"
     gene            3521..3913
                     /locus_tag="VLI90_14930"
     CDS             3521..3913
                     /locus_tag="VLI90_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15553.1"
                     /translation="MSTPHYVAEKVKDQYLLVRQDVPVATAHPEMCIGGGILMLMGLF
                     GRGPARLLCWLLGGTLLWTGLACRGKRCSGSGSRRKVRHMPRGPSYEGEDDGLRLRQK
                     PLDETDEASMESFPASDPPAKMTPTRTV"
     gene            complement(3921..8480)
                     /locus_tag="VLI90_14935"
     CDS             complement(3921..8480)
                     /locus_tag="VLI90_14935"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HSV15554.1"
                     /translation="MSTATTRPNKVLAKMLDRLFAGLMNGPGMNCRPHASRQRIDLTQ
                     LSRLSDASPEDVLRQLLSDGRVAKVIAKVKPPRRSDHGENSEGEDEPSSSQKAWDEQT
                     ALLNKLRVVADDARTYENDTGVHVLNVGFPLLSLPPGTFAAGSARSSGTKRILAPIAF
                     IPVTLTLKRGTSPSIELACRGEGVDRVMPNSALLAWLEQQTGKLEVNEIFGDDEGAHP
                     WREISDLVAHVAKALNLDVPEEYKLAPKPHTPGSEGTPDDLDASTVENDPGRREHPST
                     SSGQAPPNPAWSMDALKLTPAPRPDDDAESPAILTSAVLGLFPMANQGLLRDTQAMLA
                     QTEPLSGPVEAFLTAGIDFDDPPPIPATPDEAPPQPEQRVTRLFVTERLAAAADPCQA
                     RAVALARASRGLVVHGPPGTGKSQTITNIIADHLARGQRVLFVCEKRTALDVVHDRLD
                     HMGLGQLCGIVHDPQRDQRDLYRSIREQLEELPATKTDAGAEAKLTRVDSELQKLHED
                     LTQYHHSLMTPPQAGEPSFHELVGQWLALRGSDNQVRVNDTAASAIAPHELSARETEL
                     LDLLGRAQAISYARNPWSKCAGVAPSDYLARPMETVRTSIERCVTAAAAADATIDLAI
                     PVFSTQSPLPAQGKARAEIAERLAPLLQQMNGTAVARWAAADAAALRSAGEKLKSIES
                     SVRMFRETPLDADLRSAISPPPSAAVIPQQLRDLDAYLEIAGKWYAFLSFGAKSHAAK
                     VLQPYGLPLTPSSAERVKRFLNALRARLIIQQVRRELAGASDESAGLPADDVLDRFLQ
                     HHAELFDILLRLREEPALDGLAVLAAKALPVRAAAEAFIDGLCKSPARADTLAALEDS
                     LHATTLLDAHWLASVGQKLRNGEAASPVTDALLGQLDTLEGVLRVREARAALPEPLRK
                     LADELLSQSADPRAALDALQKSVLTGEITRRLRANPTLQAIDAHRIESNFARYRELEL
                     QKKQLVRDAVRHRWIGKQRDRLLAATGSRLNSLGASLSRRLLMSGPRAMRLRQVIAVG
                     EQIDGGDPLFDLCPVWMASPETVAQVFGRKPLFDVIIFDEASQCRLEEALPVLTRGQR
                     VVIAGDPKQLPPTRFFESAFTQSEEQNEPETEQDLFEIQQAEVEDLLGAALNLSIQQC
                     YLDVHYRSRNADLIGFSNENFYGSRLQAIPGHPRNRAKFSPLTLYRVDGVYANRGNEA
                     EADRVVQIVHDLLKRGEPPSIGIACFNLQQRDLIVERLDDRAADDAEFAKRLATARQR
                     KGADSFEGLFVKNLENVQGDERDHIIISTTYGPDAKGKFYRRFGPLGRAGGGRRLNVL
                     VTRARHEVHLVSSIPREAYLNLPAVPEGQSATGAWLLFAYLRYAEQLAALYAEDAASL
                     SEATDAKPQAADRVDVLATKSPSTFATQLAAALANDHRMGSAVHWGNDGFCVDLALRH
                     PTRPDDVTVGVLCDSCRYTAADDPVEWDIFRTGILADQGWSLRRIWTPHFFRDPSGHI
                     NSILKDAAAFVAQERPADALPTAQA"
     gene            8989..9717
                     /locus_tag="VLI90_14940"
     CDS             8989..9717
                     /locus_tag="VLI90_14940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV15555.1"
                     /translation="MSSFSRLHDEAADAHATSPVAPASSAPGGGGGGADPAVARAIDL
                     LKRSQRGDRSAYGQLVVLYQDRVFNAILRMVGDRDEAAELTQEAFTRGLSNLDSFRGD
                     AAPYTWLFRIAVNLAISRLRRVQRHRVFSLDAAGSNGRSAADRGGNSSGDQAAALIDR
                     VRQQDASNQPSHALEQRERAEQVVAALGRIDAEYRAVLVMRDVEGFDYQQMADVLGLP
                     LGTLKSRLFRARIALRDELKAYMS"
     gene            9795..12041
                     /locus_tag="VLI90_14945"
     CDS             9795..12041
                     /locus_tag="VLI90_14945"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HSV15556.1"
                     /translation="MSETPNNLDPKESTEAKLCAYLEGELPPAERAEIETYLTNNPQH
                     RQLLSELAKTREFVRALPREAAPAEIAELFQERLERSLLLDTDAAGNTAPIARMNRWP
                     HIALLAAMVCLAGGLAVVIYSVLPGHGPAPTQYSIAPQPQPMATATAPATASPSAAPT
                     ADVVAKSSGPAEKAGTPTVAGEMDLAKSGPAPVPAAAPTVVATATPPATESDHRMERF
                     GGGFEAGRVRVEKSFGGAGGGGAGFGVVSPSAISGSSSAMLGVIDTKAVNERLARSNS
                     TLQKLGAAKNNTVYVVVSANDTNAAATQVRDFFTMNNIAYQPLATDDAAVSTGVDSIA
                     LKSDATVSKQAALGMAKEEMSKKAAFDSTDGAMRKDAGPDRRLYDKDYAQKISPRAGN
                     GQDMQNAEVAANAPPAAPQSQGQSSTPTAGAPAGQAGDNLAQQQQAANYSTSNNYVQA
                     GNRNASSNVSSSYREQQVPQQPTAQAQPVQLNAAVNPADLTANSNEPVALGDSGDAWV
                     ATGLTRREAEQLSATLSTQGTGQIAAVVTPTAGGIVATGNAFNFNGTINAAATTLPAT
                     MPAQQQESQPLLFAKEPASQSATAPSGGAAADNVAVNDQLTVTVPQLVGPGVEKTNTV
                     KVAEDGTINLPMLEPVPAAGRTPAELERQIGKQYADAKLIANPTVTVTRAPATPATQP
                     VMDLPTTTQPSAVAQAVEEPQRAPATQPVLDAAERVDVVVLLQKNRIAVTPTTEPAPA
                     ATQPVEAK"
     gene            12319..>14287
                     /locus_tag="VLI90_14950"
     CDS             12319..>14287
                     /locus_tag="VLI90_14950"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HSV15557.1"
                     /translation="MFEPLEGRTLMSGAPATFSDLGNLTGRQTVGDTVSTSQAVDSYK
                     FQLPAAGSINLLLSGLTHNANLHLLNSSGGQISTSAAAGTANDQINVNSLSAGTYFAQ
                     VDFVNDGSAGTSTAYALQLTADKAGNTLATARDLGDLSVATTKTINDFVDASDTADFY
                     KFELSSIRQVNVLMNNLTANADLQVIVDVVNPGVVDTGEVVLSSSNTGTNFDQVTPTF
                     GPGTYFLKVQPAGNAATNYTLNINSLPGAVADDHGGDSASNPRAIGTLGFGTQTFVDF
                     VGSSDGADVYQFNLAHLSRINAHLDGLTDNADLQLFTAPAPSFFPETLISSSVNTNAL
                     PDDISQTVAAGTYFLRVQHVSGDTFYRLQMGVTEEDGAGDTLAAARNLGTLSNVTFNE
                     RVGGPDADDFYKFTVSAFKHVSINVNGLSTADVSLQLLDHDGQLITVSDHPGEANESI
                     VQNLVPGTYFAQVSPKIGEGPYHLAFNVTNGPDGAGNTLASAHDLGSLLHGASKTLSD
                     YVGPDDTADMFKFTLPNGSHVRSSFVTSVPGVNTFFVQDRNHDGQIQSSEKLFNAADL
                     VPGTFFFRVEKGNTTGAKYSVKLTVSDIDDGGGNTFASAGTLGNIHGNGTDATVGGYV
                     GFDDTIDIFRFDVNPFGTVTITLDHLQANADV"
BASE COUNT         2482 a         4511 c         4883 g         2411 t
ORIGIN      
        1 gcacggccga tttcaacggc acgccgctgg tcgtgccgga caacgattcg gtgctggtcg
       61 tcgcccgcgg cgggaaaggc aatcagttcg aagacggtta cgtcgtctgc ctgtcgctca
      121 gcggcggaca atacaagtgg tcaacctacg tcggcagcgc cagccacatg ttcgacatgg
      181 gcgacaacgg cgtggagcag ctgggcacgt cggagatgtg cctggcagaa ggccgcgtgt
      241 tcgtgctgac caacctgggg acggttgcgg cgctggatcc gtacgacggg cggatgatct
      301 ggctgagcgc gtatccgcgc gacgtgacgg acaatcccga gcagatgcag atggtcttcc
      361 gcaaccgcat ggccaatggc ggtcgcgcgg ggacgaacaa gccgtgggcg aacaatccgg
      421 tcatcgtcgc caacggaaaa gtcttcgtgc tgccgaccga cggcaggcac ttgctggtgt
      481 acgacgccgg caccggcgcc gaggccaatc gcatcctgct gtcggactat gacaacgccg
      541 atgtgctgct gggcgtgcgg ccgcgcatgg cgatcctcag caccgaaaag ggctgctact
      601 gcatcgattg gcgcgcctac acccacgacg acgccgagca cgcgatcatc tgggcgcgca
      661 aagacgtcgc cgccgacacc gtggcgggcg aggagaacac gatcttcggc cgcgggttcg
      721 tcaccagcga ttcggtcttc ctgccgacgc gccaccggct ctatcagctg ggctggttca
      781 agggcgggaa ggtgatgtcg atgtatccgt cgcgcggcca atggagcggc gatgaagaac
      841 cgggcaacgt gctggcgaca tcgcagggcg tgatcatcgc cgggtcgacg cgcgtggagg
      901 tttaccgcga cctgacgctg gttcgccggc agttcgagga gcggatcgcg gcgtcgccaa
      961 acgatccgca gccgcgcatt gattacgcca gcgcgctgtt cgccagcggc cagctggatg
     1021 cggcggtggc gaagctggat gaagcgatcg agatgatcgg cgggcagtcg gccatgcggc
     1081 cgggcaaggg gcgcgacctg gtcttcacca ccgcgctcag tttcgccgag cgggccgcga
     1141 aagatccggc caacgaaacc ggttcggtca cggagaagct gtacgatcgt gccgcggcgg
     1201 cggcggattc accgttggac caggcggtct atcgtctcag ccgcgcgcgc cacctgcacg
     1261 accgcaagga ctacgccggc gaagtgaagc tgtgccagga agtgttgtcg gacgacgcga
     1321 tgcgcgcggt gtcggtgaac gacaacacca ctgccggggc cgaggcggag gcggccgtca
     1381 acggcgcgat gctggccgac cgcacggcgt atgccccgat cgaacaagcc gccgccgcgg
     1441 ccgtgaaggc ggcgagcgcg gcggacgacc cgtcgcagat gctggcggtg gcgcgggtgt
     1501 atcccaactc gcgcgcggcc gccgaggccc ggcaggcggc ggcgaagttg ctcgaagcgg
     1561 ccaagcgata cgatgaggcg atcgcgacgc tgcgcgagat gtacgcgggg tcgctcgacc
     1621 ccgccgtgcg gaccaacttg ctcgtgtcgg ttgccaacga ttatctcacg atgcccggcg
     1681 gagtgggccc cgcgatcgat cggctggcga tggcgaccgc gaaggaccag gtgcaggacc
     1741 gcatgccgca ggcgctgatc ctgccggacg gcacgcggat tgagaacgcg acgttcaccg
     1801 cggcgattgg gacgctgcgc gagatgcagg agaaggaaga cgtcgcccgc ctgccggact
     1861 tccacatccc cgtgcaggtg cacgcgaagg acaaggcgtt cacccgcacc gaaggttcgc
     1921 tggtgatccc cgacgtgacg gcgctggttc gcccgctgcg cgattttccg cggaacgacc
     1981 gcgtcgtcgc gtggtcggcc accgcggggt tgtcgattta tccggtgggc gaatcgacgc
     2041 cggtgacgac gatgccggcg agcgcgctgc cggaatcgcc cggtggctgc gcgtggatcg
     2101 atccggcgaa gctagtcctc tggagcggct cgcgcgtgat gctgctgggg gacggcgcag
     2161 gggctgggcc gaagtggcag acgccgctgc cgtccggtcc ggcgctgtcc accacggtgg
     2221 cggattcgcg cggcgcgatt gacgatgagc aagtcgcgat ggaccaggcg gccggcaacg
     2281 ccggcgcggc ggggccgaac ggcatcgtca tcgacggcgg tttcggtggt ggcgggcgca
     2341 tcatcgtccg caacggccgg cagttcatca tcaacggcgg gcagatccgc ttcatcgcgc
     2401 aggggaacat cggcatcgcc gggcaacccg cggcggcgcc ggcccccgcc ggtgccgcgg
     2461 cggtgggatt ggggccgcaa atcaccatgt ttcgccccgc gggcgatcgc atcgtcctca
     2521 gcacgtcgct cggtacgttg atgggcttga aggtcaacga cggcggcatc gcctggcaga
     2581 tgcgcctggc cgaggggccg ctcacgcagg tgctggcgaa tggtagattc accgtcgcgc
     2641 ggatcgatga tcccgccggg gcgcagctga tcgttctcga cacgcccacc ggccgcgtga
     2701 tcggccggcg ccgcttcggg ccagatggta cgggcaatca gctgatgaac gtcgcgctgt
     2761 cggaagaggc gacgctggtg tggaccacgc ttgccaagct gctgagcaaa gacctgtacg
     2821 agccgtggcg caacccgccg gtggacctat cgacgcggcc gaccggcgag gcgatgaact
     2881 tcgccgggtt gaatcagccg gaccagttgc tcgtgcgcgc cggccgcgtc gtcgcgctgt
     2941 acgacggcgg aaaatttgtg cgcgtgcagg acctgtccaa tcgccggatc gatccggtca
     3001 cccccgcggc gaacccgctg gccactggcg cagacacaac cgccggttcc gccagcgtcg
     3061 cgctgcgcct ggtcggcccg cgactgttca tcaccaacgg gcgaaacctc tggcagtaca
     3121 acctggcgtc agcggacgat cattacaaga cgctgctgtt cgacctggat tatccgccgc
     3181 ggattcgcga gctgttcctg ggccaggatc acgcgatcct gctgaacgag ccggttgacc
     3241 gcgggccact ggggtcgtcg ttcgtgcagc tgctgatcca ccgccgcggg ccgatcgggc
     3301 cgggcaatgc gcgcgaggcg tcgaaccttg attactatcc gcaaatcacc gacaagtccg
     3361 gcatctttgc gtggcaggcc gtcgatggcg ggatgtacta cctgagcggc gatcaccagc
     3421 tgcatttcct tcaagcgcgg aagtaagcga tcgcgctcgc catttcgggc acggcatttg
     3481 tgtgcaggaa cctccgcggc ccacgagagg aggcgcgctg atgtctaccc cgcattacgt
     3541 ggccgagaag gtcaaagacc aatatctcct cgtgcgccaa gatgtgcccg ttgccacggc
     3601 tcatccggag atgtgcatcg gcggaggtat cttgatgctg atgggcctct tcgggcgagg
     3661 cccagcgcgg ctgctgtgct ggctgctcgg agggacattg ctgtggacgg gcctcgcctg
     3721 ccgcggcaag cggtgcagtg gcagtgggtc ccgccgcaag gtgcgccaca tgccgcgcgg
     3781 tcccagctac gaaggcgaag acgacggcct gcgcctgcgg cagaagccgc tggatgagac
     3841 cgatgaagcg agcatggagt cgttccccgc cagtgatcca ccggccaaga tgaccccgac
     3901 gcggacggtg tgattttctt ttacgcctgc gccgtcggca gcgcatccgc cggccgctcc
     3961 tgcgcgacga acgcggcggc gtctttcaga atcgaattga tgtggcccga cggatcgcgg
     4021 aagaagtgcg gcgtccagat ccgccgcagc gaccacccct gatcggccag gataccggtg
     4081 cggaagatgt cccattccac cggatcgtcg gcggcggtgt aacggcacga atcgcacagc
     4141 acgccgacgg tcacgtcgtc cggccgcgtc ggatggcgaa gcgccaggtc cacgcaaaaa
     4201 ccgtcattcc cccagtgcac cgccgaaccc atgcggtgat cgttcgccag cgctgccgcc
     4261 agctgcgttg cgaaggtcga gggagatttc gtcgcgagca catccacccg atccgccgct
     4321 tgcggcttgg catctgtcgc ttcgctcaac gacgccgcgt cctccgcgta cagcgccgcg
     4381 agttgctcgg cgtatcgcag gtatgcgaac aacagccacg cccccgttgc gctctgcccc
     4441 tccggaaccg ccggcagatt cagatatgcc tcacgcggaa tggaactcac cagatggact
     4501 tcatggcgcg cgcgagtgac caagacgttc aatcgccgtc caccacccgc gcggccaagg
     4561 ggtccgaacc tgcggtaaaa cttccccttc gcgtccggcc cgtacgtcgt gctgatgatg
     4621 atgtgatcgc gctcgtcgcc ctgcacgttc tccaggttct tgacgaacaa cccttcaaac
     4681 gagtcggcac ccttgcgctg ccgcgccgtc gccaggcgct tggcgaattc cgcgtcgtcc
     4741 gcggcgcgat cgtccaatcg ctcgacgatc agatcgcgct gctgaaggtt gaagcacgcg
     4801 atgccgatcg acggcggctc gccgcgtttg agcaggtcgt gaacgatctg cacgacgcgg
     4861 tccgcttcgg cttcgttccc gcggttggcg taaacgccgt ccacgcgata gagcgtcagc
     4921 ggtgaaaatt ttgcgcgatt ccgcgggtgc ccggggatcg cctgcagccg gctgccataa
     4981 aagttctcgt tgctgaagcc gatcaagtcc gcgttgcgcg agcggtagtg cacgtcgaga
     5041 taacactgct ggatcgacaa attcagcgcc gcccccagca ggtcctccac ctccgcctgc
     5101 tggatttcga acaggtcctg ctcggtctcc ggctcgttct gctcttcgct ctgcgtgaag
     5161 gcggactcga aaaaccgcgt cggcggcaac tgcttcggat cgccggcgat gacaacgcgc
     5221 tgcccgcgcg tcagcaccgg cagcgcctct tccagccggc actgcgacgc ttcgtcgaat
     5281 atgatgacat cgaacagcgg cttgcgccca aacacctgcg cgacggtttc ggggctggcc
     5341 atccacaccg ggcacaggtc gaacagcgga tcaccgccgt cgatctgttc accgacggcg
     5401 atcacctgcc gcagccgcat cgcccgcggg ccgctcatta gcaaccgccg gctcaagctc
     5461 gcgcccaggc tgttcaagcg tgatccggtc gccgcgagca agcggtcgcg ctgcttgccg
     5521 atccagcggt gccgcacggc gtcacgcacc agttgtttct tctgcaattc cagctcgcga
     5581 tagcgcgcga agttggattc gatgcgatgg gcatcgatcg cctgcagcgt ggggttcgcc
     5641 cgcagccggc gggtgatctc accggtgagg acggactttt gtagcgcgtc cagcgcggct
     5701 cgtggatcag cagactgcga cagcagctcg tcagcgagtt ttcgcagcgg ctccggcaac
     5761 gccgcccggg cttcgcgcac gcgcaacacg ccttccagcg tgtcgagctg accgagcagc
     5821 gcatcggtca ccggcgaggc ggcttcgccg ttgcgcagct tctggccgac cgacgcgagc
     5881 caatgcgcat cgagcagcgt cgtcgcgtgc agcgaatctt cgagcgcggc cagggtatcg
     5941 gcgcgggccg gtgatttgca aagcccgtcg atgaacgcct cggccgccgc gcggaccggc
     6001 aacgccttgg cggcgaggac tgctaaacca tcgagcgcgg gttcctctcg caatcgcagc
     6061 aggatgtcga acagttccgc gtggtgctgg aggaatcggt cgagcacatc gtccgcgggc
     6121 aggcccgcgg attcatcgct tgcgccagca agttctctgc gcacctgctg gatgatgagg
     6181 cgcgcccgca gcgcgttgag gaaccgcttc acgcgctcgg cactgctggg cgtcagcggc
     6241 aggccgtacg gctgcagcac ttttgccgcg tgggacttgg cgccgaagct gaggaacgcg
     6301 taccacttcc cggcgatttc gaggtacgca tccaaatcgc gcagttgctg cgggatgacg
     6361 gcggcgctcg gcggcggact gatggcggag cgaagatccg catccagcgg cgtttcgcgg
     6421 aacatccgca cgctcgattc gatcgacttc aacttctcac cggccgaccg aagtgcggcg
     6481 gcgtcggcgg cggcccagcg cgcgacggcg gtgccgttca tctgctgaag cagcggcgcc
     6541 agccgctccg cgatttccgc gcgggccttg ccttgtgcgg gcaacggtga ctgggttgag
     6601 aacaccggga tcgctaggtc gatcgtcgca tccgccgccg cggcggcggt gacgcatcgc
     6661 tcgattgatg tccgcacggt ttccatcggg cgcgccaggt aatcactcgg cgccacgccg
     6721 gcgcacttgg accacggatt gcgcgcatag ctaatcgcct gcgcgcgacc gagcagatcg
     6781 agcagttccg tctcacgcgc cgacagttcg tgcggcgcga tcgcgctggc ggcggtgtca
     6841 ttcacgcgca cctgattgtc acttccgcga agagcaagcc actgcccgac tagctcgtga
     6901 aacgacggct cgccggcttg cggcggggtc atcaagctgt ggtgatactg cgtcaggtct
     6961 tcgtgcagtt tttgtagctc gctatcgaca cgcgtcaatt tcgcctcggc gccggcgtcg
     7021 gttttcgtcg cggggagctc ttccagttgc tcgcggattg agcggtacag gtcgcgctgg
     7081 tcgcgctgtg gatcgtgcac gatcccgcag agttggccga ggcccatgtg gtcaaggcgg
     7141 tcgtgcacga cgtcgagcgc ggttcgcttt tcgcagacga acagcacgcg ctgcccgcgg
     7201 gccaggtggt cggcgatgat gttcgttatg gtctgactct tgcccgtccc gggtgggccg
     7261 tggacgacca ggccacggct ggcgcgcgcg agcgccacgg cccgcgcctg acacggatcg
     7321 gcggcggcgg caagccgctc ggtgacgaac agccgcgtga cgcgctgctc cggttgcggc
     7381 ggtgcttcat cgggcgtcgc cggtattggc ggcgggtcat cgaaatcgat cccggcggtg
     7441 aggaacgctt cgacggggcc gctcagcggc tcggtctgcg caagcattgc ctgcgtgtcg
     7501 cggagcaggc cctggttggc catgggaaat aagcccagca cggcactggt cagaatcgcg
     7561 ggcgattcgg cgtcgtcatc aggtcgcggc gcgggggtga gtttcagcgc gtccatggac
     7621 cacgccgggt tcggaggagc ctgccctgaa cttgtcgaag gatgctcgcg acgaccggga
     7681 tcgttttcca ctgtagaagc atccaggtcg tcgggagtac cctccgaacc cggcgtgtgg
     7741 ggcttcggtg cgagtttata ttcttcgggc acgtcgaggt tcagcgcctt tgcgacgtgg
     7801 gcgacgaggt cgctgatctc tcgccacgga tgggcgcctt cgtcgtcgcc gaagatctca
     7861 ttcacttcca gcttgccggt ctgctgttcg agccaggcga gaagcgcgct gttgggcatc
     7921 acgcgatcca cgccttcccc gcggcaggcg agctcgatgc tcgggctggt gccgcgcttc
     7981 agcgtcaacg tcacggggat gaacgcgatc ggcgcgagga tgcgcttggt gccgctgctg
     8041 cgcgcgctgc ctgctgcgaa ggtgccgggc ggcagcgaca gcagcggaaa gccgacgttc
     8101 agcacgtgaa cgccggtgtc gttctcgtac gtgcgggcgt cgtcggcgac gacgcgcagc
     8161 ttgttcagca gcgcggtctg ctcgtcccac gctttctgcg atgacgaggg ctcgtcttcg
     8221 ccttcggaat tttcgccatg gtcggatctt ctcggcggct tgaccttggc gatgaccttc
     8281 gcgactcggc cgtcgctcag cagctgccgc agcacgtctt cgggggaggc gtcgctcaag
     8341 cgcgacagtt gcgtgaggtc gatgcgctga cggctggcgt gcgggcggca gttcatgccg
     8401 gggccgttca tcagcccggc gaacagccga tcgagcattt tggccaggac tttgttgggt
     8461 cgcgtggtgg cggtggacat ccgtttctct ggtgcaaatt tgcgcgaagg cgtgcgagcg
     8521 agggtaacgc gggcgttcga tgacgtcgag caaaccggac gtgtgccgaa gcgtgtgcgc
     8581 cagctggcgg cgctggttgt ttggattcgc tgaatacgct caatccaaaa gcgcagacgc
     8641 ggtgggctct gccccttgcg tttgcgcttt gaattcagct gctttagtta tgtttgaaaa
     8701 gcagcgctct cgcttggcgc acccatcttc ggcctcgttc gaaaactcga tgctcgcacg
     8761 atgacagcaa ccttgccgcc tccggggcgt atctgctgtt gtagaaactt tcgtcgcccg
     8821 gtgggtccga taacgttgac tcgcgccagt gcggctggca tcatgcggcg ttgtcgcgtt
     8881 gcggctatgg atggccgcgg gtttgtgcgt atggatggca cgcgcgcatg tgtgtgtgcg
     8941 tttccttcac gttggcggaa ctttccggac cgccgttcgt ccaatcacat gtcgagcttc
     9001 agccgcttac atgacgaggc cgccgacgct cacgcgacgt cacccgtcgc gccggcgtcg
     9061 tctgcgcccg gcggtggtgg tggtggcgcg gatccggcgg tggcacgcgc gatcgatttg
     9121 ctgaagcgct cacagcgcgg cgatcgcagc gcgtatgggc agctggtcgt gctgtaccag
     9181 gaccgcgtat tcaacgcgat cctgcgcatg gtcggcgatc gcgacgaggc ggccgagttg
     9241 acgcaggaag cgttcacgcg cgggctgtcg aacctcgaca gcttccgcgg cgacgccgcc
     9301 ccgtatacgt ggctgtttcg catcgcggtg aacctggcga tcagccgact gcgccgcgtg
     9361 cagcggcacc gggtgttttc gctcgacgcc gcgggcagca atggccgcag tgctgccgat
     9421 cgcggcggca acagcagcgg cgaccaggcg gccgcgttga tcgatcgcgt gcggcagcag
     9481 gatgcgagca atcagccatc gcacgcgctg gagcagcgcg agcgggcaga acaggtcgtt
     9541 gcggcgctcg gccgcatcga tgccgaatat cgcgcggtac tggtcatgcg cgacgtagaa
     9601 ggtttcgatt accagcagat ggccgacgtg ctgggcctgc cgctgggaac gttgaagagc
     9661 cgcttgtttc gcgcgcggat cgcgctgcga gatgagctga aggcgtatat gagctagggt
     9721 tgaggggtca ggggttaggg gtcaggaacg catcctgacc cctgacccct gacctctgac
     9781 ccctgacccc tgcaatgtct gagaccccga ataacctcga ccccaaagaa tccaccgagg
     9841 ctaagctctg cgcttacctg gaaggcgagc tgccgccggc ggaacgcgcg gagatcgaga
     9901 cgtacctgac gaacaatccg caacatcggc agctgttgag cgagttggcc aagacgcggg
     9961 aattcgttcg tgcgttgccg cgcgaggcgg cgccggcgga gatcgcggag ttgtttcagg
    10021 aacggctgga gcggtcgctg ctgctggata ccgatgcggc ggggaacacg gcgccgatcg
    10081 cgcggatgaa tcgctggccg cacatcgcgc tgctggcggc gatggtgtgt ctggcgggcg
    10141 ggttggcagt cgtgatttat tcggtgctgc ccggccacgg accggcgccg acgcagtatt
    10201 cgattgcgcc gcagccgcaa ccaatggcca cggcgaccgc gccggcgacg gcatcgccgt
    10261 cggccgcccc gaccgcggac gttgttgcga agtcatccgg tcccgccgag aaggccggta
    10321 cgccgacggt tgccggcgag atggacttgg ccaagtccgg cccggcgcct gtgcccgcgg
    10381 cggcgccaac ggttgtcgcc acggcgacac cgccggcgac ggagtctgat catcgaatgg
    10441 aacgcttcgg cggcgggttc gaagccggtc gcgttcgcgt cgaaaagagc ttcggtggcg
    10501 ctggtggtgg cggggctgga tttggcgtcg tttctccaag tgcgatctcc ggcagctcga
    10561 gcgcgatgct cggggtcatc gacaccaagg cggtgaacga gcgactcgcc cgttccaact
    10621 caacgctgca gaagctcggc gcggccaaga acaacacggt ctacgtcgtc gtgagcgcga
    10681 atgacaccaa cgccgcggca acgcaggtgc gcgatttctt cacgatgaac aacatcgcat
    10741 atcaaccgct ggccaccgat gacgcggcag tctcgaccgg tgtggacagc atcgcgctca
    10801 agtcggatgc aaccgtttcg aagcaagccg cgctggggat ggccaaggaa gagatgagca
    10861 agaaggccgc attcgattca acggacgggg cgatgcggaa ggatgccggg ccggatcggc
    10921 ggttgtacga caaggattac gcgcagaaga tttcgccgcg cgcgggcaat ggtcaggaca
    10981 tgcagaacgc cgaggtggcg gcgaatgcac cgccggcggc gccgcaatcg caggggcaat
    11041 catcgacacc caccgccggc gcgccggcgg gacaagcggg ggacaacctc gcccagcagc
    11101 agcaggcagc gaattattct acgagcaaca attacgttca ggcgggcaat cgcaacgcgt
    11161 ccagcaatgt gtcctccagc tatcgcgagc agcaagtgcc gcaacaaccg accgcgcagg
    11221 cgcaaccggt gcagctgaac gcggcggtga atccggcgga cttgacggcc aactcaaatg
    11281 agccggtagc gctgggcgac agcggcgacg cgtgggtggc gacgggtctg acgcggcgtg
    11341 aagcggagca gctgagcgcg acgttatcga cgcaggggac ggggcagatc gccgcagtgg
    11401 tcacgccgac ggcgggtggg atcgtcgcga cggggaacgc gttcaacttc aacggcacga
    11461 tcaacgcggc ggcgacgacc ctgccggcga cgatgccggc gcagcagcaa gagtcgcagc
    11521 cgctgctgtt cgcgaaggaa ccggcgtcgc aatcggcgac ggcgccgtcg ggcggggcgg
    11581 ctgcggataa cgtcgcggtc aacgatcagc tgacggtgac ggtcccgcag ttggtggggc
    11641 cgggggttga gaagacgaac acggtgaagg tggcggagga cggcacgatc aacctgccga
    11701 tgctggaacc ggtgcccgcg gcggggcgaa cgcccgcgga gttggagcgg cagatcggga
    11761 agcagtatgc cgatgcgaag ttgatcgcga atccgacggt gacggttacg cgcgccccgg
    11821 cgacgccggc gacgcagccg gtgatggatt tgccaacgac cactcaaccg tcggccgtcg
    11881 cgcaggcggt tgaagaacca cagcgagcgc cggcgactca gccggttttg gatgccgcgg
    11941 agcgagtgga cgttgtcgtg ctgcttcaga agaatcggat cgccgtcaca cccaccaccg
    12001 agccggcgcc cgcagcgaca cagccagttg aggcgaagta gaagcggatg cgccgctaac
    12061 gccgatctca catcgcctgc cttcttagcg tcgtccatag cgctcgcggg gagtagaagc
    12121 gtcttgccta tttagaacca gcccatgacc gattgaggaa taaggggtta cgaatattcg
    12181 ccaccgccaa caaacccgcc gctttccagc gttgatgttc acgaataggg ggttcgaaat
    12241 ctcgtgaacc agaaggaaag cagtatgtca cgccagatca agaatcgttc gcgttcgatc
    12301 gttcgccggg cctgtgccat gtttgaaccg ctcgaaggcc gcacgctgat gagtggcgcg
    12361 ccggcgacgt ttagcgacct tggcaacctt acgggccggc agaccgtcgg cgacaccgtt
    12421 tccacttcgc aggcggtgga ctcttataag ttccagctgc cggcggcggg cagcatcaac
    12481 ctgctgctca gcgggctgac gcacaacgcg aacctgcatc tgctcaattc gtccggtggc
    12541 cagatcagca cgagcgcggc ggccggcacg gccaatgatc agatcaacgt caatagcctc
    12601 tcggcgggga cttacttcgc gcaggttgat ttcgtcaacg acggctcggc cggcacgagc
    12661 acggcgtacg cgctgcagct gacggcggac aaggcgggga acacgctggc gacggcgcgc
    12721 gacctcggcg atctttcggt cgcgacgacc aagacgatca acgacttcgt cgatgccagc
    12781 gacaccgcgg acttttataa gttcgagttg tcgtcgatcc gccaggtgaa cgtgctgatg
    12841 aacaacctga ccgccaacgc ggacctgcag gtgatcgtgg acgtggtcaa ccctggcgtg
    12901 gtcgatacag gcgaggttgt gctctcatcg agcaacaccg gcacgaattt cgatcaggtg
    12961 acgccgacgt tcggtcccgg cacttacttc ctgaaagttc aaccggcggg gaacgcggcg
    13021 acgaattaca cgctgaacat caacagcctg ccgggcgcgg tcgccgatga tcacggcggt
    13081 gattcggcga gcaacccgcg ggcgatcggc acgctgggtt tcggcacgca aacgttcgtg
    13141 gacttcgtcg gcagctctga cggcgcggat gtgtaccagt tcaacctggc gcatctgagc
    13201 cggatcaacg cgcatctgga cggcctgacc gacaacgccg acctgcagct gttcacagcg
    13261 ccggcgccga gctttttccc cgagacgttg atcagcagct cggtcaacac caacgcgctg
    13321 ccggacgaca tctcgcaaac cgtggcggcg gggacgtact tcctgcgcgt gcagcacgtg
    13381 agcggcgata cgttctatcg cctgcagatg ggggtgacgg aagaagacgg cgcgggcgat
    13441 acgcttgccg ctgcgcgcaa cctgggcacg ctgagcaacg tcacgttcaa cgaacgcgtc
    13501 ggcgggcccg acgccgacga cttctacaag ttcacggtgt cggcgttcaa gcacgtgagc
    13561 atcaacgtca acggcttgag caccgccgac gtcagccttc agctgctgga ccacgatggc
    13621 cagctgatca ccgtcagcga tcatccgggc gaggcgaatg agtcgatcgt gcagaacctc
    13681 gtgccgggca cgtacttcgc gcaggtctcg ccgaagatcg gtgaggggcc gtaccacctc
    13741 gcgttcaacg tgaccaacgg tcccgacggc gccggcaata cgctggcgag cgcgcacgac
    13801 ttgggcagcc tgttgcacgg cgcgagtaag acgctgagtg attacgtcgg tcccgacgac
    13861 acggccgaca tgttcaagtt cacgctgccc aacggcagcc acgtgaggag cagcttcgtc
    13921 acgtccgtcc ccggcgtgaa cacgttcttc gtgcaggatc ggaatcacga cggccagatc
    13981 cagagcagcg agaaattgtt caacgccgcc gatctggtgc cgggcacgtt cttcttccgc
    14041 gtggagaagg gaaatacgac gggcgccaag tacagcgtca agctgaccgt gagcgacatc
    14101 gatgacggcg gcgggaacac gttcgccagc gccggcacgt tgggcaacat ccacggcaac
    14161 ggcacggacg cgaccgtcgg cggttacgtc ggcttcgacg acacgatcga tatcttcagg
    14221 ttcgacgtga acccgttcgg taccgtgacg atcacgctgg atcatctgca ggcgaatgcc
    14281 gacgtta
//
LOCUS       DATIMH010000864         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_203949, whole genome shotgun sequence.
ACCESSION   DATIMH010000864 DATIMH010000000
VERSION     DATIMH010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203949"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..282)
                     /locus_tag="VLI90_14955"
     CDS             complement(40..282)
                     /locus_tag="VLI90_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15558.1"
                     /translation="MNITRVGYRHNDLYTAYLDAGAPAGVPGKPYLLPDDVLAKLRPA
                     CDGAPESRKIKTSPDQTISLDLPLNENDVYLLTIQP"
     gene            412..903
                     /locus_tag="VLI90_14960"
     CDS             412..903
                     /locus_tag="VLI90_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15559.1"
                     /translation="MEDQAFDVVPGARLSFSAVTSRRGQMDRCTNCGETIGKLETPFV
                     WKDSVVCRRCFGRLSSATEPLVAAADLEAEAKAPDHYGGGMTQAARKMGERTAPVGPN
                     PIICPNPNCGYRGPGRVKKKGSAVVMVLLLLLWILPGILYAIFYNGSVIVCPRCGMKV
                     RDA"
     gene            1018..1185
                     /locus_tag="VLI90_14965"
     CDS             1018..1185
                     /locus_tag="VLI90_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15560.1"
                     /translation="MPSQKREDFDSKLIFVVIGTVAAIALGSRFSGITSFIVTAGGFA
                     LSLFAAQYFEM"
     gene            complement(1224..2642)
                     /locus_tag="VLI90_14970"
     CDS             complement(1224..2642)
                     /locus_tag="VLI90_14970"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15561.1"
                     /translation="MNLLQLALSVSCMLATVTSVTLAATGPATSGEIPMRQVNVDVTR
                     DRGALNRAFRFSIGSDRAIIHLRPEHQRDLRFLKQTCGFEYMRFHGLLNEEMHVVQQS
                     ATGEITYDWTNIDRVYDLLVRELQIRPIVELGFMPEPLASGKQTIFWWKGNVTAPRSY
                     DQWGTLIEQLARHLTQRYGADEVRRWYFEVWNEPNLNGFWPAGQAEYFKLYETTARAI
                     KRVDPQYRVGGPATAGYGWIKQTLDFCTQHDVPIDFIATHAYGAMEGFLDEHGKGHTM
                     LAPAPMSVIDGLPGVLGDIQHSQWPALPVLVTEWGPSYSPRDPVHDSYFCAAWILHRL
                     RRLPAGVLAMSYWTFSDQFEESGLPNKPFHGGFGLLTMQGLPKPAYFAYRFLNQLGDT
                     ELACTDENTWACRDAHGGVQLLLWDYHHPKQDQPNAQFFARDWQANSLPGVRINIANL
                     PRSRTTAARDSMLADRPTPTCR"
     gene            complement(2687..>3211)
                     /locus_tag="VLI90_14975"
     CDS             complement(2687..>3211)
                     /locus_tag="VLI90_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15562.1"
                     /translation="ITIKPSVPRPVVFWDFTNPRVIDTQTFTSNYALVEDLAQRANRA
                     VARVDLPPGGVVPREKANELLKVARLPEEDKLNRANIRGVVFDVMTAPDFTCDDPDAG
                     VMVVMQSSANWWMPLGNIRLRDAKAWKTQQFDLKTPEQIKAMPAALNIMFLLNANKPV
                     KGSIYFDRVGFMVR"
BASE COUNT          599 a          905 c         1056 g          651 t
ORIGIN      
        1 attttgccgt ggccgcaaaa ggcggagttg atttcgcgat tacggctgga tcgtgagcag
       61 atacacgtcg ttctcgttca gcggcagatc gagcgagatg gtttgatccg ggctcgtctt
      121 gatctttcgg gattccggcg cgccgtcgca agcggggcgg agcttggcca gcacgtcgtc
      181 gggtagcagg tacggcttgc cgggcacacc cgcgggggcg ccggcgtcga gataggcggt
      241 gtagaggtcg ttgtgcctgt agccgacgcg cgtgatgttc acggtgtatt ccgccagccc
      301 cgtagctgta gctggccgga gaaaagctac cgccagttga cgacggggaa gtgaactgcc
      361 cgctgaacag attgcgggcg ggcgaactgt aactgtcgcc agataaagcg aatggaagat
      421 caggcatttg atgttgttcc gggggcgcgg ttaagctttt cggcggtcac ttcacggcga
      481 gggcagatgg atcgatgcac gaactgcggc gaaacgattg gtaaactcga aacaccgttc
      541 gtctggaaag acagcgtggt ttgtcggcga tgcttcggac gattaagcag cgctacggag
      601 ccgctggtcg ctgctgctga tcttgaagct gaagcaaagg ctcccgatca ttacgggggc
      661 ggaatgactc aggcggcacg taagatgggt gaacggacag cgccggttgg tccgaacccg
      721 atcatctgcc caaatccaaa ctgcggatat cgtggaccgg gcagggtgaa gaaaaaggga
      781 tcggcggtcg tgatggtcct gctgttgctg ctctggattt tgccgggaat cctgtatgcg
      841 atcttttaca acggcagcgt gattgtctgc ccgcgatgcg ggatgaaggt gcgggacgct
      901 tgaccgtcgc ctagcgaatc gcttgcggaa tcatttccgg gctgccgcct tctgtttttc
      961 cggctcgacc ttggcgagct tcaacagcgc tgtccatttc ggctacgctt gattcgtatg
     1021 ccgagtcaga agcgggaaga tttcgattct aaactgattt tcgtggtgat tggaaccgtt
     1081 gccgcgattg cccttggcag cagattcagc ggcattactt catttatcgt gacggcgggc
     1141 ggattcgccc tgtcgctctt tgcggcgcag tatttcgaga tgtagtccac tagaaacaaa
     1201 accgcccacg ccgttgcgac cggctaccgg catgtcggag tcggtcgatc agcgagcatc
     1261 gaatctctcg cggcggtcgt gcgcgaccgt gggaggttcg cgatgttgat gcggacgccg
     1321 ggcaatgagt tggcctgcca atcgcgcgcg aagaactggg cgttgggctg atcttgcttg
     1381 ggatgatggt agtcccaaag cagcaactgc acgccgccgt gtgcgtcgcg gcaggcccag
     1441 gtgttttcgt cggtgcacgc caactccgtg tcgccgagtt ggttgaggaa acgataagcg
     1501 aagtacgccg gcttgggcag gccctgcatg gtgagcagac cgaacccgcc gtggaagggt
     1561 ttgttcggaa ggccggattc ttcgaattga tcggagaagg tccagtagga catggccagc
     1621 acacccgcgg gcaggcggcg caggcgatgc aggatccatg cggcgcagaa ataggaatcg
     1681 tgcacgggat cgcgcggcga atagctcggt ccccactcgg tgaccagcac gggcagcgcc
     1741 ggccattggc tgtgctgaat gtcaccgagc accccgggca agccgtcgat gaccgacatc
     1801 ggcgcggggg caagcatcgt gtgacccttg ccgtgctcgt caagaaaacc ttccatcgcg
     1861 ccgtacgcgt gggtcgcgat gaagtcgatc ggcacgtcgt gctgcgtgca gaagtcgagc
     1921 gtctgcttga tccagccata acccgccgtc gccggaccgc cgacgcggta ttgcggatcg
     1981 acgcgcttga tggcccgcgc cgtcgtttca tacagcttga aatactccgc ttgcccggcg
     2041 ggccagaagc catttaagtt cggctcgttc cagacttcga agtaccaccg gcggacttcg
     2101 tccgcgccgt agcgctgggt cagatgccgc gcgagctgct cgatgagcgt gccccattga
     2161 tcgtaagatc gcggagccgt gacgttcccc ttccaccaga aaatcgtctg ttttccgctg
     2221 gccagcggtt cgggcatgaa ccccagttcc acgatggggc ggatctgcaa ttcgcgaacg
     2281 agcaagtcgt agacgcggtc gatgttggtc cagtcgtagg tgatttcgcc ggtggcggat
     2341 tgctggacga cgtgcatctc ctcgttcagc aggccgtgga agcgcatgta ttcgaagccg
     2401 caggtttgct tcaggaagcg caggtcgcgc tggtgctcgg gccgcagatg gatgatggcg
     2461 cgatcggagc cgatcgagaa gcggaacgcg cggttcagcg cgcccctgtc gcgcgtgaca
     2521 tcgacgttaa cctggcgcat ggggatctcg ccagaagtgg cagggcccgt cgcggcgagc
     2581 gtgaccgatg tcaccgtggc aagcatgcac gagaccgaca acgccagctg aagcagattc
     2641 atgcagatgt ccttattgca cgacacacac acgcacgtgg gccacatcat cgcaccatga
     2701 acccaacgcg gtcgaagtaa atcgagcctt tcaccggctt gttggcgttc aggaggaaca
     2761 tgatgttgag cgccgccggc attgccttga tctgctccgg agtcttcaag tcgaattgct
     2821 gcgtcttcca cgctttggcg tcgcgcagcc gaatgttgcc cagcggcatc caccaattcg
     2881 ccgagctttg catcaccacc atcacgccgg cgtcgggatc gtcgcaggtg aagtccggcg
     2941 ccgtcatgac gtcgaacacc acgcctcgga tattcgcgcg gttcagctta tcttcttccg
     3001 gcaggcgtgc gaccttcagc agttcgttcg ccttctcgcg ggggacgacg ccgccgggcg
     3061 gcagatctac ccgcgccacg gcgcgattcg cgcgctgcgc caggtcctcg acgagcgcgt
     3121 agttcgacgt gaacgtctgg gtgtcgatga cgcgcgggtt ggtgaagtcc cagaagacca
     3181 cgggccgggg aacggacggc ttgatcgtga t
//
LOCUS       DATIMH010000865         4590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3093864, whole genome shotgun sequence.
ACCESSION   DATIMH010000865 DATIMH010000000
VERSION     DATIMH010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3093864"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2766)
                     /locus_tag="VLI90_14980"
     CDS             complement(<1..2766)
                     /locus_tag="VLI90_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSV15563.1"
                     /translation="MPSPAPARHARSPRPSLRRRSALRRAIAPGMLIAAAAAAAPSAF
                     GYNSLASQFTLVAIPDPQYYAVVQWKNDQYYAGQMNWIVNNAAAKNIGYVVGLGDNVQ
                     DGNPFTTNADGSIGTTYTGNIIPQSGSPSVTGAPSINSVDPTNHDFEAEWKRAAADWN
                     ILSTANIPHYTVIGNHDYYHWDQKKDPSEWNKYFASSMYTGKPWFTGGLSPADSTQAT
                     GLKIYSGLNAYNYFTAGGYKFLNIGLQFVPTAGDIAWAQSIINANKGLPTIISTHDYQ
                     DTNGRDGAGNNLWNNFISKAGNSQIFMVLSGHVNGVHQQTSTDADGKSVFEVLSDYQD
                     FSFSGLANGGGYLRTMAFDTSANTIHVQTYSPVANNFLTTGSGATANDFTLSLDFASR
                     FGPPPLATGKTFYWDPGHGANQAGGGAGTWDTSTNANWFGGSADVMWSTATASDSAVF
                     GGATGGAVTVAAAGVSVNDIVINTAGYSFGGGTITLVGSPSITTPAAGAVTVANVLAG
                     SAGLTVKGGTTTLTAANTFTGGLSITNGASLIFTADNNLGASGQPITIDGGTLTYNGT
                     VAGGTQISPRAINIGGNDATINVPNPGPGFGGQGKLVFNTGATISGAGDITKAGPGWL
                     TVYTNNTAYGGNWTVNGGVLELGNANAAGTGAITVNNGGEVALNLQTTQLAIPVTLNA
                     GATISTDNHTASNGTFSGPVTANGNFNVRLGDFYGPTSESVTISGPISGSGQATLIGA
                     AGTTSSTGRLNLSGDNSSFSGGIVIPTGTVAVGPSAGRPLGTGPITLSGGKLALQGQT
                     VQTGAAQAVAPIGVSGFNKDVIYGLPDSNGTTTVGIDNVFSFYQTNYIPPTGTLNAGT
                     EFTTGGLDDQNITSAVGGAGTPFQLQSFTANNVLQFNQGSTAALTLKTPAVFTKLSVL
                     ATSSFANE"
     gene            complement(2849..>4590)
                     /locus_tag="VLI90_14985"
     CDS             complement(2849..>4590)
                     /locus_tag="VLI90_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV15564.1"
                     /translation="AFTGGFAVPTGTLVAGLSRSNTLGTGGITLSGGKLALQGQQTPT
                     GTPAAAAAVAVTGFNKDVIYGNPDSSSFSTNTSGLDNVFGFYQTGYTPQFNPTTTIQN
                     AGTQLSSGGLPGQNITSALAGSKPFSFQSFMANNALAIAKGSTGTLSLSTPAAFANLS
                     LLASSTNAADDTPNVTINFADGGSVTTTYKAYDWFIGTDTLRQNASVFGAAGVFRYSA
                     STAPGWDQRAFGLYESDIDLTNINGVDYSKRPIASLTFAATSATNGQTDVFAVSGAAR
                     AWANGASQSYANNVSVTADSGIDVSGSLAATLGTLNIGSNKLSVTSADATSSPYSLSF
                     GATTVSGAATIDVAPSTGGGVGTVTLGNVCGGGSITKTNTGTLKFAGTTNAISGNLAI
                     NGGNAVVAFSPGGGATPASLSVALLSFSGGKLDLTDNKLFTTLPVANVKSAMSANTLF
                     TSSTGGVLGYMDTGSGTTEVRFTLPGDTNLDGAVDVADLGALATSYGTTAGALWAQGD
                     ANGDGAVNVADLGLVATNYGQSIGAGTVDASSAAVASSTAVVAGSASVPEPTSLGLIG
                     GLSIAVAARRRRR"
BASE COUNT          681 a         1463 c         1562 g          884 t
ORIGIN      
        1 ctcgttggcg aacgaactcg tcgccagcac cgacagcttc gtgaacaccg ccggagtctt
       61 cagcgtcagc gcggcggtgc tgccctggtt gaactgcagc acgttgttgg ccgtgaacga
      121 ctgcagctgg aatggcgtgc ccgcgccacc gacggcgctg gtgatattct ggtcgtccaa
      181 tccgccggtg gtgaattcgg tgcctgcgtt cagcgtgccg gtcgggggga tgtaattggt
      241 ctgatagaag ctgaagacgt tgtcgatgcc gacggtggtg gtgccgttgg aatcgggcag
      301 gccgtagatg acgtccttgt tgaaaccact gacgccgatc ggtgcgacgg cttgcgcggc
      361 cccagtctgc accgtctggc cctgcagcgc cagcttaccg cccgacagcg tgatcggccc
      421 ggtgcccagc ggccggcccg ctgatggacc taccgcgacg gtgccggtcg ggattacaat
      481 cccgccggag aaactggaat tgtcgccgct gagattcaac cggccggtcg atgaagtcgt
      541 gcccgcggcg ccgatcagcg tcgcctgtcc gctccccgag atcggcccgc tgatcgtcac
      601 tgattcactc gtcggcccgt agaaatcgcc gaggcgaacg ttgaagttcc cgttcgcggt
      661 gacgggtccg ctgaaggtgc cgttggacgc ggtgtgattg tcggtgctga tcgtcgcgcc
      721 ggcgttcaat gtgaccggaa tcgccagctg cgtcgtctgc aggttcagcg ccacttcgcc
      781 gccgttgttc acggtgatcg cgccggtgcc ggcggcgttg gcgttgccca gctccagcac
      841 accgccgttc acggtccagt tcccgccgta agcggtgttg ttcgtgtaga cggtcaacca
      901 accgggaccg gccttggtga tgtcaccggc gccgctgatg gtcgcgccgg tgttgaagac
      961 gagcttgcct tgcccgccga atccgggacc gggattgggc acgttgatcg tcgcgtcgtt
     1021 cccgccgatg ttgatcgcgc gcgggctgat ctgcgtgccg ccggctaccg tgccgttgta
     1081 cgtgagcgtg ccgccatcga tcgtgatcgg ctggccgctg gccccgaggt tgttgtcggc
     1141 ggtgaagatc agcgaggcgc cgttggtgat cgacaggcca ccggtgaacg tgttggcggc
     1201 ggttagcgtg gtcgtgccgc ccttgaccgt caatcccgcg ctgcctgcca gcacgttggc
     1261 gacggtgacc gcgccagccg cgggggtggt gatgctcggc gaaccgacga gcgtgatcgt
     1321 tccgccgccg aaggaataac cggcggtgtt gatgacgatg tcgttcacgc tgacgcccgc
     1381 ggcggccacg gtgactgcgc caccggtggc gccaccgaag acggccgaat ccgaagccgt
     1441 cgccgtcgac cacatcacgt ctgcactgcc gccgaaccag ttggcgttgg tcgaggtgtc
     1501 ccacgtgccc gcgccaccgc cggcctgatt cgcgccgtgg ccggggtccc agtaaaaggt
     1561 cttgcccgtc gccagcggcg gtggaccgaa gcgcgacgcg aagtccaggc tcagcgtgaa
     1621 gtcgttcgcc gtcgcgccgc tgccggtcgt gagaaagttg ttggccaccg gcgaataagt
     1681 ctgcacgtga atcgtgttcg cgctggtgtc gaacgccatc gtgcgcaggt acccaccgcc
     1741 gttggccagc ccgctgaagc tgaagtcctg gtagtcgctg agaacttcaa acacgctctt
     1801 gccgtcagcg tcggttgacg tctgctggtg cacgccgttc acgtgcccgc tcagcaccat
     1861 gaagatctgc gaattgccgg ccttggaaat gaagttattc cagaggttgt tccccgcgcc
     1921 gtcccgaccg ttggtgtcct ggtaatcgtg cgtcgagatg atcgtgggca ggcccttgtt
     1981 ggcgttgatg atgctctgcg cccacgcgat gtcaccggcc gtcgggacga actgcagccc
     2041 gatgttaagg aacttgtagc cgccggccgt gaaatagttg tacgcgttga ggccggagta
     2101 aatcttcagc cccgtcgcct gcgtgctgtc ggccggcgac agcccgccgg tgaaccaggg
     2161 cttgccggtg tacattgacg acgcgaagta cttgttccac tcgctcgggt ccttcttctg
     2221 gtcccagtgg tagtaatcgt gattgccgat gaccgtgtag tgcgggatgt tcgcggtgga
     2281 gaggatgttc cagtccgccg ccgcccgctt ccactcggct tcgaaatcgt gattggtcgg
     2341 atcgaccgag ttgatgctgg gcgcgcccgt gactgacggc gaaccgctct gcgggatgat
     2401 gttgcccgta taggtcgtgc cgatgctgcc gtcggcgttg gtggtgaacg gattgccgtc
     2461 ctgcacgttg tcgccgagcc cgaccacata cccgatgttc ttcgcagccg cgttgttgac
     2521 gatccaattc atctgccccg cgtaatactg gtcgttcttc cactggacga ccgcgtagta
     2581 ctggggatca ggaatcgcca cgagcgtgaa ctggctggca aggctgttgt agccgaaggc
     2641 gctgggcgca gcggcagcgg cggcggcgat cagcattccc ggcgcgatgg cgcggcgcag
     2701 cgccgaacgg cggcgcagag aggggcgagg cgagcgggca tgtcgagcag gtgcgggcga
     2761 cggcattgac gatcctccga gggcgggcaa gtgaaagagt ctcaaatcgc gttcttctga
     2821 attcggggtc atccgcgaag tccatggttc agcgccgccg gcggcgggcg gcgacggcga
     2881 tcgacagtcc accgatgagg ccgagcgatg tcggttccgg caccgacgcc gagcccgcga
     2941 cgaccgcggt tgaactggcg acggcggcac tgctggcgtc caccgtgcca gcgccgatgc
     3001 tttgtccgta gttggtcgcc accaggccca ggtcggccac gttgaccgcg ccgtcgccgt
     3061 tcgcgtcgcc ttgcgcccac agggcgccgg cggtggtgcc gtagctggtt gccagcgccc
     3121 ccaggtccgc cacgtccacc gcgccgtcca ggttcgtgtc gccgggcaac gtgaagcgaa
     3181 cttccgtcgt gccgctgccg gtgtccatgt agcccagcac accgccggtg ctgctggtga
     3241 acagcgtgtt ggccgacatg gccgacttca cgttcgccac cggcaaagtc gtgaacagct
     3301 tgttgtcggt caggtcgagc ttcccgccgc tgaagctgag cagggcgacg ctgagcgacg
     3361 ccggcgtcgc gccaccgccg gggctgaagg cgacgacggc gttcccgccg ttgatcgcca
     3421 ggttgccgct gatcgcgttg gtcgtgccgg cgaacttcag cgtgccggtg ttggtcttgg
     3481 tgattgagcc accgccgcac acgttgccca gggttacggt gcccacgccg ccgccggtgc
     3541 tgggggcgac gtcaatcgtc gcagcgccgc tcaccgtcgt cgcgccgaag ctcaggctgt
     3601 atggactgct cgtcgcgtcc gcgctggtga cggacagctt gttcgagccg atgttcagcg
     3661 tgcccagcgt cgcggccagc gacccgctca cgtcgatgcc ggaatccgcc gtcacgctca
     3721 cgttgttcgc gtagctttgg cttgcgccgt tggcccaggc gcgagcggcg ccgctgacgg
     3781 cgaagacatc cgtctgcccg ttggtcgccg acgtcgccgc gaacgtcagg ctggcgatcg
     3841 ggcgcttcga ataatcgacc ccgttgatgt tcgtcagatc gatgtcgctt tcgtacaggc
     3901 cgaacgcgcg ctggtcccag cccggtgccg tgctggcgct gtagcggaag acgccggccg
     3961 cgccgaagac gctggcgttc tgccgcagcg tgtcggtgcc gatgaaccag tcgtatgcct
     4021 tgtacgtcgt ggtcacgctg ccgccgtcgg cgaagttgat cgtgacgttc ggcgtgtcgt
     4081 ccgccgcgtt ggtgctcgac gccagcagcg acaagtttgc gaacgccgcc ggcgtcgata
     4141 acgacagcgt gccggtgctg cccttggcaa tcgccagcgc gttgttcgcc atgaacgact
     4201 ggaacgagaa tggcttgctg cccgccagcg cgctggtgat gttctgcccc ggcaatccgc
     4261 cgctcgacaa ttgcgtcccc gcgttttgaa tcgtggtcgt cggattgaac tgcggcgtgt
     4321 acccggtctg gtaaaacccg aacacgttgt cgagccccga cgtgttggtc gagaagctgc
     4381 tcgaatcagg attgccgtag atcacatcct tgttgaaccc cgtgaccgcg acggccgccg
     4441 ccgccgcggg agttccggtt ggcgtctgct ggccctgcag cgccagtttg ccaccggaca
     4501 atgtgatgcc gccggtgccc agcgtattgg acctactcaa ccccgcgacc agcgtgccgg
     4561 tcggcacggc gaagccgccg gtgaacgcgg
//
LOCUS       DATIMH010000866         6421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5693958, whole genome shotgun sequence.
ACCESSION   DATIMH010000866 DATIMH010000000
VERSION     DATIMH010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5693958"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..2692)
                     /gene="clpB"
                     /locus_tag="VLI90_14990"
     CDS             complement(56..2692)
                     /gene="clpB"
                     /locus_tag="VLI90_14990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HSV15565.1"
                     /translation="MRFEKLTIKAQEAVARAQEVAQRNDHAEILPLHVLSGLLAETDG
                     VVLPLLQKLGVQIPRIQQVVADELERLPKATGTQLGMNRATNDVFDRAQKEADRLKDE
                     YVSTEHLMLGLAQVKSSAQTILGGEGVDHASILNALKEVRGGARVTDQNPEDKYQALQ
                     RYGRDLVEMARQGKIDPVIGRDEEIRRTMQVLSRRTKNNPVLIGEPGVGKTAIVEGLA
                     IRMLNGDVPAVLANKRIIALDMGLLIAGAKYRGEFEDRLKAVIKEVTQSEGEIILFID
                     ELHTVVGAGKAEGAMDAGNLLKPALARGELRTIGATTLDEYKKHIEKDAALERRFQPI
                     IVGEPSVEDTIAILRGLKERYETHHGVRITDGAIVSAATLSSRYITERFLPDKAIDLI
                     DEAASRLRIENDSMPQELDEIRRRVMQLQIEIEALRREKDSASKQQLAKAEKELADLQ
                     ERNNALTARWENEKKSMDATKHLREELDRKRVELEQAQRQGNWEVAARIQYGEIRDLQ
                     TKLTDAEKRVKELTSSGKSLVKEEVGSEQIAEVVSRWTGVPVSKMLEGDRERLMKMEQ
                     RLGQRVIGQEDAVEAIANAVRRNRAGLGDPNRPIGSFLFLGPTGVGKTELTKALAEFL
                     FDDESAMVRIDMSEFMEQHSVARLIGAPPGYVGYEEGGRLTEAVRRRPYSVILFDEVE
                     KAHRDVFNVLLQVLDDGRLTDGQGRTVNFKNTVVIMTSNIGSQEIQKLATHQAPEWEV
                     EAAVKNLLKDYFRPEFLNRIDEVILFHSLSKEQLSRIVDVQLEGLRKRLAGRNLKLAL
                     SDDAKKLLADEGYDPTYGARPLKRVIQQRLENALATKILGGEFADGDTVHVDVDVAKH
                     DFTFRRGNEVVEGELVEEHAAH"
     gene            2980..3699
                     /locus_tag="VLI90_14995"
     CDS             2980..3699
                     /locus_tag="VLI90_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15566.1"
                     /translation="MSTITLSASPAYPAVLNPTTLTFNTSTATLDLTNNELITTDTPA
                     SIRTKIIAGQIFTSTPNGVLGYAEIGNNHTAVRYTIAGDVNLDGSVNIDDLGQLATNC
                     GTTTGGSWSTGDFNFDNAVHVSDLGVLATHCGHSLDNATAPAATPAATQSAVPEPAML
                     AALASIVLRGRRPQGLGRIRRMLSSPTPGSEVLRRYSGGTPTTRMLLRARKRPGSSEY
                     LRTRRGGRRKMTALYSPTAFP"
     gene            3760..4278
                     /locus_tag="VLI90_15000"
     CDS             3760..4278
                     /locus_tag="VLI90_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15567.1"
                     /translation="MSLREQGQIVCMGHTWNVRGNIRDVYCLVADPRSYTQWLRVVQQ
                     VDLVGPAPRVAVGHAMIMRVRSLLPYRLLWHVKLVRLEPGRVIDTECRVTLGSRVSLH
                     GRVRFRFKQIGDRVIVTNDQQLQLQRRLPRWIAPLAQWIFRVNHAWAMSRARRHLQRR
                     VRDSSVAGACVA"
     gene            4719..>6421
                     /locus_tag="VLI90_15005"
     CDS             4719..>6421
                     /locus_tag="VLI90_15005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HSV15568.1"
                     /translation="MEPPAKPVMVGPQADGAGVLVPTRQLLHPAGRSVSFHGRPVDLV
                     LSRNGRTAFVKSDLSLFVIDTVTNAVRQEVKYPPKIGASLHGIVLSADESHLYVTLAQ
                     SLLADFSVGGDGTVAPGRTISLHVKKEGESYPCGIALLGHSNTAVVCLSCNNSIAIVD
                     LSAGKIVANIPVGLAPYDVTVARDGASAYVSNWGGRRAGAGDKTGISAGTPVVVDDRG
                     IACSGSVSVVDLRSRKQVNEIPTGLHPSAVLLDREGKTLYVANANSDTISVIDTANAR
                     VTRTISVRPDVDLPFGSAPNALALTEDQNALLIANGGNNAVAVVPLKDAGDRPAAFIP
                     TGWYPGALAMHGDQLFIANIKGIGTRDPAVAGKWSTRFGAWGSVSSLAIPNDPATLER
                     YSKQVLADAMVPQTLAAMERMRAGDRPAAAVPARAGEPSVFHHVVYIIKENRTYDQVL
                     GDIGRGNSDPALCIYGRDVTPNQHALAEQFVLLDNYYCNGILSTDGHHWATEGITTDY
                     LEKSFGAWVRSYPSTGDDPLAYSASGFIWDNALLHGKSFRNYGEFGTAALPAKANFEQ
                     I"
BASE COUNT         1083 a         2110 c         1986 g         1242 t
ORIGIN      
        1 aaacacgggc cggtggcgcc gtgccacgac acgggaaaga tgcccgtgcc acgagttagt
       61 gcgccgcgtg ctcttccacc aactccccct ccaccacttc gtttccgcgg cggaaggtga
      121 agtcgtgctt tgcgacgtcc acgtccacgt gcaccgtgtc gccatctgcg aactcgccgc
      181 cgaggatttt tgtcgccagc gcgttttcca gccgctgctg gatgacgcgc ttcagcggcc
      241 gggcaccgta cgtcgggtcg tagccttcgt cggccagcag cttcttcgca tcatcgctca
      301 gcgccagctt caggttgcgg ccggctaagc gcttccgcag gccttccagc tgcacatcca
      361 cgatccggct cagctgctcc ttgctcagcg agtggaacaa aatcacctcg tcaatgcggt
      421 tcaggaactc ggggcggaag taatccttca gcaggttctt caccgccgct tccacttccc
      481 actcgggcgc ctggtgtgtc gccagcttct ggatttcctg cgacccgatg ttgctcgtca
      541 tgatgacgac ggtgttcttg aagttcaccg tccggccctg gccgtcggtc aaccggccgt
      601 cgtccagcac ctgcagcagc acgttgaaca cgtcgcggtg cgccttctcg acttcatcga
      661 acaggatcac gctgtacggc cggcgccgaa ccgcttcggt caaccgcccg ccttcttcgt
      721 aaccgacgta acccggcggc gcgccaatca atcgggcgac cgagtgctgc tccataaact
      781 cactcatgtc gatgcgcacc atcgcgcttt catcgtcgaa caaaaactca gccagcgcct
      841 tggtcagctc ggtcttaccg acgccggtgg gaccgaggaa caggaacgag ccgatcgggc
      901 gattgggatc gcccagcccc gcgcgatttc ggcgaacggc attggcgatc gcttcgacgg
      961 cgtcttcctg gccgatcacg cgctggccca accgctgctc catcttcatg agccgctcgc
     1021 gatcgccttc gagcatcttg ctgaccggca ccccggtcca gcgcgagacg acctcggcga
     1081 tctgctcgct gcccacttct tccttcacca gcgacttacc gctggacgtc aactctttga
     1141 cgcgtttctc ggcatccgtc aatttcgtct gcagatcgcg gatctcgccg tactggattc
     1201 gcgcggcgac ttcccagttg ccctgccgct gcgcctgctc cagctcgacg cgcttgcgat
     1261 ccagctcttc gcgcaggtgc ttggtcgcat ccatcgactt cttttcgttc tcccagcgcg
     1321 cggtcagcgc gttgttgcgc tcctgcaggt cggccagttc cttctccgcc ttggccaact
     1381 gctgtttgct ggccgagtct ttttcgcgtc gaagcgcttc gatctcgatc tgcagctgca
     1441 tgacgcgccg gcggatctcg tccagctcct gcggcatcga atcgttctcg atgcgcaacc
     1501 gcgacgccgc ttcgtcgatc aagtcgatcg ccttgtccgg caggaagcgc tcggtgatgt
     1561 agcgcgagct gagcgtcgcc gccgagacga tcgcgccgtc ggtgatgcgc acgccgtggt
     1621 gcgtttcgta acgctccttc agcccgcgca ggatggcgat ggtatcttcc acgctcggct
     1681 cgccgacgat gatcggctgg aaccggcgtt ccaacgccgc gtccttctcg atgtgcttct
     1741 tgtattcatc cagggtcgtc gcaccgatcg tgcgcagctc gccgcgtgct agcgccggct
     1801 tcagcaaatt ccccgcgtcc atcgcgcctt cggctttgcc ggcgccaacg acggtgtgca
     1861 gctcgtcgat gaacaggatg atctcgcctt cgctctgcgt cacttccttg atgaccgctt
     1921 tgagacgatc ttcaaactcg ccgcggtatt tcgcgcccgc gatgagcagg cccatgtcca
     1981 gcgcgatgat gcgcttgttg gccagcaccg ccgggacgtc gccgttgagc atgcgaatgg
     2041 ccaaaccttc gacgatggcg gtcttgccga cgccgggttc gccgatcagc acgggattgt
     2101 tcttcgtgcg gcgcgacagc acctgcatcg tccggcgaat ctcttcatcg cgaccgataa
     2161 ctgggtcgat ctttccctgc cgggccatct cgaccaggtc gcggccatag cgctgcagcg
     2221 cctggtattt gtcttcaggg ttctgatcgg tcacgcgtgc cccgccgcgc acttctttca
     2281 gagcgttcag gatggacgca tgatccacgc cttcaccgcc caggatcgtc tgcgcgctgc
     2341 tcttcacctg cgccaggccg agcatcaggt gctcggtcga gacgtattcg tctttcagtc
     2401 gatccgcttc cttctgagcg cgatcgaaca catcgttcgt cgcgcggttc attccgagct
     2461 gcgtgccggt cgccttgggc aaccgctcga gctcgtccgc cacgacttgc tggatgcgcg
     2521 ggatctggac ccccagcttc tgcagcagcg gcaggacgac gccgtcggtt tcggcgagca
     2581 accccgacag cacatgcagc ggcagaattt ccgcgtggtc attccgctgg gccacttcct
     2641 gcgcgcgggc aacggcctct tgagctttga ttgtgagttt ctcgaatcgc attcgtagtt
     2701 acctccaaga ccctttcggg cgaacaggta agagcaaaga taatgccatt gcgacggatt
     2761 gacggagcga tggcgtgaat ggaagtgcag cttcgcacaa aatttagggc ctacgaacag
     2821 atcgatgtgg cgcgctcaag tctttggcac tgccacatca cgttgcaggc tgcgccgtct
     2881 gcacaaatgg cagttgcttt caatcggatc atcaaaccac gcgcgccgcc agcggctcac
     2941 cgacgcgcgc ttgccttgct cacgctgaac agcagcagcg tgtccaccat tacgctgtcc
     3001 gcatcccccg cctaccccgc cgttctgaac ccgacaacgc tgacgttcaa cacgagcacc
     3061 gcaacgctcg acctgaccaa caacgagctg atcaccaccg acacacccgc ctccatccgc
     3121 accaagatca tcgccggcca aatctttacc agcaccccca acggcgtgct cggttacgcc
     3181 gagatcggga acaaccacac cgcagtccgc tacacgattg ccggcgatgt gaacctcgac
     3241 ggctccgtca acatcgatga cctcggtcag ctggctacga actgcggcac gacaaccggc
     3301 ggcagctgga gcaccggcga cttcaacttc gataacgcgg ttcacgtctc agacctgggc
     3361 gtcctcgcga cccattgtgg ccattcgctc gacaatgcca ccgcacccgc cgccaccccc
     3421 gcggcaactc aaagcgcggt gccggaaccg gcaatgctcg cggcgctggc gtcgattgtc
     3481 ttacggggac gacgcccgca agggctcggc aggatcagac gcatgttaag ctcccccacg
     3541 ccgggttcgg aggtactccg gaggtactcc ggaggtactc cgacgacccg gatgcttctg
     3601 cgcgcaagaa agagacccgg gtcgtcggag tacctccgaa cccggcgtgg ggggcgacgc
     3661 aaaatgacgg cgctttatag ccccaccgcg tttccataat cgcaaaggct gccttggcac
     3721 cgcgtatatg gccggtcact caacgaccag gagaccgcga tgtcgctcag ggaacaggga
     3781 caaatcgttt gcatgggcca cacgtggaac gtgcggggca acatccgcga tgtttactgc
     3841 ctggtggccg acccgcgttc atacacgcaa tggctgcgcg tcgtgcagca ggtggatctc
     3901 gtcggtccag cgccgcgcgt ggccgtgggg catgcgatga taatgcgggt gcgatccctg
     3961 ctgccttacc gactgttgtg gcacgtcaag ctcgttcgtc tcgaacccgg ccgggtgatc
     4021 gacaccgagt gccgggtgac cctgggcagc cgggtgtcgc tccacgggcg cgtgcgcttc
     4081 cgcttcaaac agatcggcga tcgcgtgatc gtgacaaacg atcagcagct gcagctgcaa
     4141 cgccggctgc cgcggtggat cgcgccgctt gctcagtgga tctttcgagt aaaccacgca
     4201 tgggcgatga gccgcgcccg ccggcacctg cagcgccggg ttagggacag ctccgtcgcc
     4261 ggcgcgtgcg tggcgtgaat tcaaaggccg gggcggcggc gacgacggct ggcgatgccg
     4321 gtaattgccg gcatggccag cgcaccgacc gccatgggct ccggcacggg cacgctgacc
     4381 gtgccgttgg tgtaaagctg gctggtgtcc cacgatttcc cgccggggag cggagtcgta
     4441 gcgaatgact tggatcgtct gccacgccgg gtgcggagcg tactcccgac ggagcgagat
     4501 tcttcctttc tcgacggccg atccgggacg tcgggaatac gctccgaacc cggcgtgagt
     4561 ggaagcgggc gcttggttcc ttcgacgcgt cgcttgtcaa agtgatccgt cgcgggtacg
     4621 cgatagggat catgtcatac gcaatttgtt tcgggcggat gttatgcatc gtggggctgg
     4681 tggcggttgg ggggatggcg ctgtcttgtc agcatgctgt ggaacctccc gcgaaaccgg
     4741 tgatggtggg gccgcaggcg gatggcgctg gcgtgctcgt gccgacgcgg cagctgcttc
     4801 accccgccgg gcgcagcgtg agctttcatg gccggcccgt cgatctcgtg ctgtcgcgca
     4861 atgggcgcac cgcgtttgta aagagcgacc tgtcgctgtt cgtcatcgac acggtgacca
     4921 acgcggtgcg gcaggaggtg aagtacccgc cgaagatcgg ggcgtcttta catggcatcg
     4981 tgctatcggc ggatgaatcg cacctgtacg tgacgctggc gcagtcgctg cttgcggact
     5041 tttccgtcgg cggagacggc acggtagcgc cgggccgaac gatttcactg cacgtgaaga
     5101 aggaaggcga aagctatccc tgcggcatcg ccctgctggg ccattccaac accgccgtcg
     5161 tttgtctttc gtgcaacaac agcatcgcta tcgtcgatct gtccgcgggg aagatcgtcg
     5221 cgaatattcc ggtgggactg gcgccatatg acgtgacggt cgcgcgcgac ggcgcgagcg
     5281 cgtatgtgtc gaactggggc ggccgacggg ccggggccgg ggacaagacc ggcatctccg
     5341 cggggacgcc ggtggtcgtg gatgaccgcg gcatcgcgtg tagcgggagc gtttccgtcg
     5401 tcgatctgcg atcgcgaaag caggtgaacg agattccgac ggggctgcat ccgtcggcgg
     5461 tgctgttgga tcgcgagggt aagacgctct atgtcgccaa cgccaacagc gacacgatca
     5521 gcgtcatcga caccgcgaac gcccgcgtga cgcgcacgat cagcgtgcgg cccgatgtcg
     5581 atctgccctt cggctctgcg cccaacgcgc tggcgctgac ggaggatcag aacgcgctgc
     5641 tcatcgccaa cggcgggaac aacgcggtgg cggttgtgcc cctgaaggat gccggcgatc
     5701 gccccgccgc gttcattccg acgggctggt atcccggcgc gctcgcgatg cacggcgacc
     5761 agctgttcat cgcgaacatc aaaggcatcg gcacgcgcga tcccgcggtg gcggggaaat
     5821 ggtcaacgcg ctttggcgcg tggggctccg tgtcatcgct ggcgattcca aacgacccgg
     5881 ccacgctgga gcgatatagc aagcaggtgc tggcggacgc gatggtgccg cagacgctgg
     5941 cggcaatgga gcggatgcgc gccggcgatc gacctgccgc tgcggtgccg gcgcgcgcgg
     6001 gcgagccgtc ggtcttccat cacgtcgtgt acatcatcaa ggagaatcgc acgtacgatc
     6061 aggtgctggg cgacatcggc cgggggaact ccgatccggc gctgtgcatt tacgggcgcg
     6121 acgtgacgcc gaaccagcat gcgctcgccg agcagttcgt gctgctggac aactattatt
     6181 gcaacggcat cctgtccacc gacggccatc actgggcaac cgagggcatc accaccgact
     6241 atctggagaa atcgttcggc gcgtgggtgc gatcgtaccc gagcaccggc gacgatccgc
     6301 tggcgtactc ggcgtccggc ttcatctggg acaacgcgct gctgcacggc aaatcattcc
     6361 gcaactacgg cgagtttggc accgccgcgt tgccggccaa ggcgaacttc gagcagattt
     6421 a
//
LOCUS       DATIMH010000867         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3094054, whole genome shotgun sequence.
ACCESSION   DATIMH010000867 DATIMH010000000
VERSION     DATIMH010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3094054"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /gene="gmk"
                     /locus_tag="VLI90_15010"
     CDS             complement(<1..576)
                     /gene="gmk"
                     /locus_tag="VLI90_15010"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HSV15569.1"
                     /translation="MATTNQHAGLLIVLVGPSGVGKSTISRSLAEKMHLKYIASATTR
                     KHTDKDDIGKTYEYVDERTFIRKLENGEFLEYAKVWDAYYGTPKHPTLDYLARGEDVL
                     LEIDVQGALQVRYQYPDALMIFIMPPDQQSLLQRLRDRGRETEDDIEKRFRGAKREIW
                     MAKGSRAFDDMVINDNVDRAVEEISRLIRQKK"
     gene            complement(651..1532)
                     /locus_tag="VLI90_15015"
     CDS             complement(651..1532)
                     /locus_tag="VLI90_15015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HSV15570.1"
                     /translation="MIVSMTGFGDATAERNGAHYAVEIRSLNNRFFKPVIKLPETVSG
                     LEPELETMLREKLGRGSITYILKMRMDSAEAAYHINTQALKSYLEQLQSVKGIDRLVQ
                     IDLASLVQLPGVCQEPRDETDEIQKHGDTIRELTTKGIAKLQAMREREGRALFDELMK
                     HVNVIATALTEISKRAPSVIEDYHKRLQQRVNQLLSKAELRVNETDLIKEVAVFAERA
                     DIAEEIQRLSSHLSAFEDACRKDEHGGRKLDFIAQEMLREANTIGSKANDAAIARNIV
                     EIKGAIDRLKEQVQNVE"
     gene            complement(1655..1993)
                     /gene="secG"
                     /locus_tag="VLI90_15020"
     CDS             complement(1655..1993)
                     /gene="secG"
                     /locus_tag="VLI90_15020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSV15571.1"
                     /translation="MSFLFYLFLALLIVVSIFLILLILIQKGRGGGLSTAFGGGGGSS
                     AFGTKTGDVLTWATSIIFGVFILLAVAVTLVVNAQYGHAGSTPGTPTNGPTAPNGPQS
                     PAPLPPPLGT"
     gene            complement(2067..2816)
                     /gene="tpiA"
                     /locus_tag="VLI90_15025"
     CDS             complement(2067..2816)
                     /gene="tpiA"
                     /locus_tag="VLI90_15025"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HSV15572.1"
                     /translation="MARTKFVAGNWKMNTTKASATELAKAVAKGAPAGVQAGVAPPFV
                     YLDVVGQALAGSSVMLGAQDVYFEKNGAFTGEISVEMLKDLGVKFVLTGHSERRHVLK
                     ESGELVAKKADAVYAGGLTLVHCVGEKLEERDANQTFAVVQRQLDELKPDKMQDPDRL
                     VIAYEPVWAIGTGRNASDAQAQEVHAYIRGAIAKSWNKDFADRVRIQYGGSMKPENAA
                     GLLQQPDVDGGLIGGAALKADSFLAIANAAK"
     gene            complement(2892..3953)
                     /gene="aroF"
                     /locus_tag="VLI90_15030"
     CDS             complement(2892..3953)
                     /gene="aroF"
                     /locus_tag="VLI90_15030"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSV15573.1"
                     /translation="MNHSAGQAAIDRVVAEIEKMGNKAHLSRGEFRTVIGAVGEEGTL
                     DQVHLSSLDGVEKVVPIMKPYKLASREFHEADTVVEIGGGRVPVVKVGGKHAACIAGP
                     CAAENEQMVTRIARHVRDGGATVLRGGVYKPRTSPYSFQGLGLEALKWMRAVGDDLGM
                     PICVEVMDTRQVADMEPFVDCFQIGARNMQNFDLLREVGKTRKAVLLKRGLAATVKDW
                     LLSAEYVLSQGNRNVVLCERGIKTFEDSIRYTLDVTSIPVAKQWTHLPVIVDPSHAAG
                     KRDYVPALATAGMAAGAHGVIVEVHYSPSEAQCDGPQALLPEVFKDLMVQLRKIAAIF
                     GKEFAEIGEPVKPRPLVAV"
BASE COUNT          781 a         1448 c         1295 g          946 t
ORIGIN      
        1 tttcttttgt cgaatcagcc gggagatttc ctccaccgcc cggtccacgt tgtcgttgat
       61 gaccatgtcg tcaaacgccc ggctgccctt ggccatccag atctcgcgct tcgcgccgcg
      121 gaaacgcttt tcgatgtcgt cttccgtctc gcggccacga tcgcgcaatc gctgcagcag
      181 cgactgctgg tctggcggca tgatgaaaat catcagggcg tccggatatt gataccgcac
      241 ctgcagcgcg ccttgcacgt cgatctccag cagcacgtct tcgccgcggg cgaggtagtc
      301 cagcgtcggg tgcttgggcg tgccataata ggcgtcccaa accttggcgt attcgaggaa
      361 ttcgccgttt tccagcttcc ggatgaacgt tcgctcgtcc acgtattcgt acgtcttgcc
      421 gatgtcgtcc ttgtccgtgt gcttgcgcgt cgtcgcgctg gcgatgtact tcagatgcat
      481 cttctcggcc aggctgcgcg agatggtgct cttgcccacg ccgctgggac cgacgaggac
      541 gatcaggagt ccggcatgtt ggttggtcgt ggccatgttg cgtttgtcag ttgtcagttg
      601 tcagtggtcc gttgcaacaa ctgacaacgg acaatggacc actgacaatc ctactcaacg
      661 ttctgcacct gttccttaag ccgatcgatt gcgcctttga tctcgacgat gttccgcgcg
      721 atggcggcgt cgttcgcttt gctgccgatc gtattcgctt ctctcagcat ttcctgggcg
      781 atgaaatcga gctttcggcc gccgtgttcg tccttgcggc aggcgtcttc aaacgcgctc
      841 aagtggctgc tcagccgctg gatttcctca gcgatgtcgg cgcgctcggc gaagaccgca
      901 acctccttga tcaggtccgt ctcgttcacg cgcagctcgg ctttgctcag cagctggttc
      961 acgcgctgct gcaaccgctt atgatagtcc tcgatcaccg atggggcgcg cttcgagatt
     1021 tccgtcagtg cagtcgcgat cacgttcacg tgcttcatca attcgtcaaa cagcgcccgg
     1081 ccttcgcgct cgcgcatcgc ctgcagcttg gcgatgccct tggtggtcag ctcgcggatc
     1141 gtgtcgccgt gcttttgaat ttcgtccgtc tcatcgcgcg gctcctggca gacaccgggc
     1201 aattgcacca ggctggccag gtcgatctgc accagccggt cgattccctt gaccgattga
     1261 agctgttcca aatacgactt cagcgcctgc gtgttgatgt gatacgccgc ctcggcactg
     1321 tccatccgca tcttcaggat gtacgtgatc gacccgcggc ccagcttttc gcggagcatc
     1381 gtctccagct ccggctccag gccgctgacg gtctcgggca gcttgatgac cggcttgaag
     1441 aagcggttgt tcagcgaacg gatttcgacg gcgtaatgcg cgccgttccg ctcggcggtc
     1501 gcgtctccaa aaccagtcat gctgacgatc atgcgtgaac cttgttcagg taaaatggca
     1561 aaaggaaaat ggcaaatggt cggaagccga gcaccatttg ctattcacat tttccattgg
     1621 ccatttgcca ttttccattc gcgatagcta gagatcaggt gcccagcggg ggtggcaatg
     1681 gcgctggaga ctgtggtccg ttcggcgcgg tcgggccatt cgtcggcgtc cccggcgtgc
     1741 tgccggcatg accgtactgc gcgtttacga ccaacgtcac cgcgacggcg agcaggatga
     1801 agacgccgaa gatgatgctg gtggcccagg tgagcacgtc accggtcttg gtgccgaaag
     1861 cgctgctgcc gccaccgccg ccaaacgccg tggacaaccc gccgccgcga cccttttgga
     1921 tcaggatcag caggatcagg aagatcgaca cgacgatcag caaggcgagg aagaggtaga
     1981 aaaggaaact cattgttttt tgttccgtag aggcgacgcc tgcgtcgccc ttggttgcaa
     2041 tgggcgacgc acgcgtcgcc cctgcatcat ttcgccgcgt ttgcgattgc caggaaactg
     2101 tctgccttca gggcggcacc gccgatcagg ccgccgtcga cgtccggctg ctgcaacagt
     2161 ccggcggcgt tttcgggctt catgctgccg ccgtactgga ttcgtacgcg gtccgcgaaa
     2221 tccttgttcc acgacttggc aatcgcgccg cgaatatacg cgtggacttc ctgcgcctgc
     2281 gcatcgctgg cgttgcggcc ggtgccgatg gcccagacgg gctcgtatgc gatcacgagg
     2341 cgatcggggt cctgcatctt gtctggcttc agctcatcca gctgccgctg gacgactgcg
     2401 aacgtctggt tcgcgtcgcg ctcttcgagc ttctcgccca cgcagtgcac cagcgtcaat
     2461 ccaccggcgt acaccgcatc ggccttcttc gcgaccagct cgccggattc cttgagcacg
     2521 tgccggcgtt cgctgtgacc ggtgaggacg aacttcacgc cgaggtcctt gagcatctcg
     2581 acggaaatct cgccggtgaa cgcgccattc ttttcgaagt acacgtcttg tgcgccgagc
     2641 atcacgctcg atcccgccag tgcttgaccc accacgtcga gatatacgaa cggcggggca
     2701 acgccagcct gaacacccgc gggcgcaccc ttggcgacgg ccttggccaa ctcggtggca
     2761 gaggctttgg tcgtgttcat cttccagtta ccggcgacga atttcgtgcg agccatcgtt
     2821 tcgttttcgt ataaagccgc gaccagtggt tgcggggggg gactctttcg aatgtcaaag
     2881 cgcgacggaa atcagaccgc gaccaatggt cgcggcttta caggttcgcc aatctccgcg
     2941 aattccttcc caaagatggc cgcgatcttc cgcaactgca ccatcaggtc cttgaacact
     3001 tccggcagca gcgcctgcgg gccgtcgcac tgcgcttcgc tcggactgta atgcacttcg
     3061 acgatgacgc catgcgcgcc cgccgccatc ccggccgttg ccagcgccgg cacgtaatcg
     3121 cgcttccccg ccgcgtgcga tggatccacg atcaccggca gatgcgtcca ctgcttggcc
     3181 accggaatgc tcgtcacgtc cagcgtgtat cgaatgctgt cctcgaacgt cttgatgccg
     3241 cgctcgcaca ggacgacgtt gcggttgccc tggctcagca cgtactcggc cgacagcagc
     3301 caatccttca ccgtcgcggc caagccgcgc ttcaacagca ccgctttacg cgtcttgccg
     3361 acttctctca gcaggtcgaa gttctgcatg ttgcgcgcgc cgatctgaaa gcaatcgacg
     3421 aacggctcca tgtccgccac ctgccgcgtg tccatcactt ccacgcagat cggcatgccc
     3481 aggtcatcgc cgaccgcgcg catccatttg agcgcttcga gcccgagtcc ctggaacgag
     3541 tagggcgagg tccgcggctt ataaacgccg ccgcgcagga ccgtcgcgcc accgtcgcgc
     3601 acgtgacgcg cgatccgcgt caccatctgt tcattctcgg cagcgcacgg cccggcgatg
     3661 cacgcggcgt gcttcccgcc gaccttcacc accggcacgc gaccgccgcc gatctcgacg
     3721 acggtgtccg cctcgtgaaa ctcccggctg gccagcttgt acggcttcat gatcggcacg
     3781 actttttcca cgccgtccag cgacgacagg tggacctggt ccagcgtccc ttcttcgccc
     3841 actgcgccga tgacggtgcg gaattccccg cgggagaggt gggccttgtt gcccatcttt
     3901 tcgatctccg cgaccacgcg gtcgatcgcg gcctgcccgg cggaatggtt catcacaatg
     3961 atcacgagcg aacactccct cgggcgaaac tgcgcccggc acacctaccg acccgcgcac
     4021 atcccaaaag tgcggaaacc gctgtaagtc gagcaaagga cgactcgcag gcgaagctcc
     4081 gcccgccgaa agtcgagccg agcatattaa tggaaggggg ggatatctac aagatgtgtc
     4141 ggatcgcttt aagacgcggt ggatcatctc ccatagcagt agcccccgac gccggtgtct
     4201 gaggtactca gaaggcacgg gttgtttgcg taactgatcc gggtcttcgg agtccctcaa
     4261 acaccggcat cccgggccat atccgaacca tgctttcctc ccaagccttt taacccaagg
     4321 accgccgggc cagtccggga aatgcaagct aaatgggctg tgttcgttcg cgccatggta
     4381 gacccacaag aatggcgggg gatgacgatc ccaacgcgcg atctgctgag ccgtcggagg
     4441 gctgttgtcc acgagaaatg tcgcccagaa
//
LOCUS       DATIMH010000868         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3671815, whole genome shotgun sequence.
ACCESSION   DATIMH010000868 DATIMH010000000
VERSION     DATIMH010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671815"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VLI90_15035"
     CDS             <1..284
                     /locus_tag="VLI90_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15574.1"
                     /translation="TAVMVNSNLEQYKIAGARDVPQIEAILVENYAGKSCTDAAGLAE
                     PAIVATPAAIANAVYNAIGVRVRELPMTPARVLAALAGKTATAERSAGA"
     gene            281..1309
                     /locus_tag="VLI90_15040"
     CDS             281..1309
                     /locus_tag="VLI90_15040"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HSV15575.1"
                     /translation="MNAFEWTQASTVDEALASQSEDSVFKAGGVDLMDMLKERLIEPP
                     RIVNLRTVHELDFVEDDAGGGLRVGPLVTLARVAAHPKIKAKWPILADAVSRAATPQI
                     RNMATLGGNLLQRPRCWYFRSEQFPCRRKGGEVCYAQEGENQYHAIFNNQLCAIVHPS
                     AAATPLVALGGSVVLKSSKGEREVPAEKFFVLPNQDLHNENTIKPGEILTAIKLPAPA
                     GGSQSAYVRQGEKESFDWPIAEVAVVIDRDGDVCRRASIVLGAAAAAPHRAVNAEELL
                     TGKRIEEALAADAAKAALAGAHPMTQNGYKLPLFQALIRRTILAAVGIDAVGGDAVGS
                     AADFGGAR"
     gene            1306..1563
                     /locus_tag="VLI90_15045"
     CDS             1306..1563
                     /locus_tag="VLI90_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15576.1"
                     /translation="MSDTTHKSTGVHLPIHNAPVPTVCAMLRSRGGTDDQFDGVDWRH
                     GRSMTANYWCLATMESVGPDDHYVHAHECRAGRRCFRKPSE"
     gene            complement(1674..>2000)
                     /locus_tag="VLI90_15050"
     CDS             complement(1674..>2000)
                     /locus_tag="VLI90_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15577.1"
                     /translation="IVLGPVTRRMSGDDGICNYFTAGNGDVVRFTRRRSRGSWPMPWE
                     WFRIMGAGRKRGRFVYHRFTWANPTGQQLTLSWRDEQWLYRGAREWIDAYIERIVEMR
                     ISKARS"
BASE COUNT          371 a          643 c          670 g          316 t
ORIGIN      
        1 agacggcggt gatggtgaat tcgaacctgg agcaatacaa gatcgctggt gcgcgcgacg
       61 tgccgcagat cgaagcgatc ctggttgaga attacgccgg caagagctgc acggacgccg
      121 cgggtttggc cgaaccggcc atcgtcgcga cgccggcggc catcgccaat gccgtgtaca
      181 acgcgatcgg tgtgcgcgtg cgcgagctgc cgatgacacc ggcccgggtg ctggcggcac
      241 tggccggcaa gaccgccacg gccgaaagga gcgccggagc atgaacgcct tcgaatggac
      301 gcaagcatcg accgtggacg aggcgctggc cagccagagc gaagactccg tcttcaaagc
      361 cggcggcgtt gacctgatgg acatgctgaa ggagcggctg attgaaccgc cgcgcatcgt
      421 caacctacgc acggtgcacg agctggactt cgtcgaagac gacgccggcg gtgggctgcg
      481 cgtcgggccg ctggtgacgc tggcgcgcgt ggcggcgcat ccgaagatca aggcgaagtg
      541 gccgatcctg gccgacgccg tcagtcgcgc cgcgacgccg cagatccgca acatggccac
      601 gctcggcggg aacctgttgc agcggccgcg ctgctggtac tttcgcagcg agcagttccc
      661 ctgccgccgc aagggcgggg aggtctgcta cgcgcaggaa ggcgagaacc agtatcacgc
      721 gatcttcaac aaccagctgt gcgcgatcgt ccatccgtcc gccgcggcca cgccgctggt
      781 ggcgctgggc ggcagcgtgg tgctcaagag cagcaagggc gagcgcgaag tgccggccga
      841 aaagttcttc gtcctgccga accaggacct gcacaacgag aacacgatca agccgggcga
      901 gatcttgacg gccatcaagc tgccagcgcc cgcggggggc agccagtcgg cgtacgtgcg
      961 ccaaggcgag aaggagtcgt tcgattggcc gatcgcggaa gtcgccgtgg tgatcgatcg
     1021 cgacggcgat gtttgccggc gtgcatcgat cgtgctcggc gccgcggcgg cggcgccgca
     1081 tcgcgctgtt aatgcggagg aactgttgac cggcaaacgc atcgaagaag cgctggcggc
     1141 cgacgcggcc aaggccgcgt tggcgggcgc gcatccgatg acgcagaacg gttacaagct
     1201 gccgttgttc caggcgctga ttcgccgaac gatcctggcg gccgtcggca tcgacgcggt
     1261 cggcggcgac gccgtcggtt ccgccgccga tttcggaggt gcccgatgag cgacacgacg
     1321 cacaaatcca ccggcgttca cctgccgatc cacaacgccc ccgtccccac cgtctgcgcg
     1381 atgctccgct cccgcggcgg caccgacgac caattcgacg gcgtcgattg gcgccacggg
     1441 cgatcgatga ccgccaacta ttggtgcctg gctacgatgg aaagcgtggg gccggacgat
     1501 cactacgtcc acgcacacga atgccgcgcg ggccggcggt gcttccgtaa accgagcgag
     1561 tgacggctga atgatgaatg cagaatacaa aatgaagagg cgaatcacct gtcttcattc
     1621 tgcgttctgc gttctgcatt ctgcattcgg tcattgatgc gggtctttcg tgctcaagac
     1681 cgtgccttcg aaatccgcat ctccacgatc cgctcgatat atgcgtcgat ccactcgcgc
     1741 gccccgcggt agagccattg ctcgtcgcgc cagctcagcg tcagctgttg gccggtggga
     1801 ttggcccacg tgaagcggtg gtacacgaac cgaccgcgct tgcggccggc gcccatgatg
     1861 cgaaaccatt cccacggcat aggccagctg ccgcggcttc gccgccgggt aaagcgaacg
     1921 acgtcaccat tgccggctgt gaaatagttg cagatgccat catcgccgct catgcgcctg
     1981 gtcactggcc ccagcacgat
//
LOCUS       DATIMH010000869         7529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2804229, whole genome shotgun sequence.
ACCESSION   DATIMH010000869 DATIMH010000000
VERSION     DATIMH010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2804229"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VLI90_15055"
     CDS             complement(<1..482)
                     /locus_tag="VLI90_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV15578.1"
                     /translation="MSNMNTQTIGKSGLKSTRLAYGNMRSAGTWEPKAVTAEMRSAGV
                     RAHVAALEAGYTLFDTADIYCRGVCEEILGQALREVSGMRERIVIATKCGIRFAGDPT
                     PDAPHRYDFSAKHIVWSCEQSLRRIGIETIDLYHLHRPDVLMDPTEIAEAFDQLKRAG
                     "
     gene            595..912
                     /locus_tag="VLI90_15060"
     CDS             595..912
                     /locus_tag="VLI90_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15579.1"
                     /translation="MTTGSTATKRCITCGKDVTTIKRMKDSQGRYWCVDCGMADRRKK
                     MKAASKSKSVSNSGGGVSLMERLRDHTGGGGAGGTDKGRLVKMLVAVAIMAAIAGWQF
                     LSH"
     gene            complement(980..2368)
                     /locus_tag="VLI90_15065"
     CDS             complement(980..2368)
                     /locus_tag="VLI90_15065"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSV15580.1"
                     /translation="MSCSTTTLRGPILNPLPSGTVEFHQNGALQWDEHGRITYIGDAA
                     HLPATPARNVQSCGVILPPFLDCHIHIPQWPIRGRFCDGVVGCPAEGRLLAGLNRNVF
                     PYEARCAEPVHTIETVAAFREDTLAHGVVGGAAYMTVHAPAVAQALHDLPATWSVGLV
                     LMNMNCPAYLRTDETNLDRDVESLAREFGRRMIVTDRFAVAVDSPLRRRAVALAERFG
                     LRTQTHLNEQLREKELVERTLYPDAASYTDVYARDGLPQREAIMAHCIHMRDAEFDQL
                     AAAGAVIAHCPTSNSLLGSGIMRLDGVIDRNIPYAICTDVGASPTTSILCEMAQFLKV
                     HAGRSRRATPSEALVRTTIKAAEMLGLADQLGTFAPGRPASFIEVDCDVDALRGASAD
                     EAILTALLAMNQSGPPARPEFESLAKGSLDTGAELDWITNDFNAAAERLDRKVNRVTL
                     EGQSAWERTTAK"
     gene            complement(2442..3791)
                     /gene="allB"
                     /locus_tag="VLI90_15070"
     CDS             complement(2442..3791)
                     /gene="allB"
                     /locus_tag="VLI90_15070"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HSV15581.1"
                     /translation="MNHDLLIRNGHLVTPSGVHRADVAVADGKVVEFASQIAGSAREE
                     IDATGLHVFPGLIDIHVHFNDPGRADWEGFDTGSAALAAGGGTCFFDMPLNSSPPVID
                     AAAFDGKLTAATGRSYADFGLWGGIVPGNLDQMEALADRGVVGFKAFMSNSGIEDFPR
                     TDDLTLYRAMRKAKQLDLPVAVHAESDTMTSMLAAEAMAAGHTSVAAYLDSRPIVAEV
                     EAVERAIILAEETGCRLHIVHVSNSRSIEQVRRAAARGECDVTCETCPHYLALNRHDV
                     ERLGASAKCAPPLRTPAENDELWQDLAAGKIPIIASDHSPSPPGMKTDANFFKVWGGV
                     AGVQSTLPILLSRGPALPLPLVAKVTAATPASRFRIAGKGKIEAGFDADFALVDVTGC
                     FELKREQLLDRHKLSPYIGRCFHGVVRRTILRGMTIFQDGATVNAARGQLVKPQRRR"
     gene            complement(3940..4293)
                     /gene="uraH"
                     /locus_tag="VLI90_15075"
     CDS             complement(3940..4293)
                     /gene="uraH"
                     /locus_tag="VLI90_15075"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HSV15582.1"
                     /translation="MPGKLTTHVLDTANGTPAGGMRIELHRLEADKKLPLADVVTNRD
                     GRSDAPLLSGDAMATGHYRLLFHVGNYFTAKGSPDAKRFLDAVPVLFRIDDANAAYHV
                     PLLVSPWAYSTYRGS"
     gene            complement(4302..5150)
                     /gene="pucL"
                     /locus_tag="VLI90_15080"
     CDS             complement(4302..5150)
                     /gene="pucL"
                     /locus_tag="VLI90_15080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HSV15583.1"
                     /translation="MSIKLTANHYGKSDVRLTKVVRRGAHHDLFEFSVDVELRGDFAA
                     SYTAGDNSKIVATDSMKNTVYVLAKERDFNSAEQFAILLAEHFTKTYSHVETATVTVR
                     QSSWSRINTNGKPHDHAFVSGGAELHTAVATSTRSGAATIAGGVQDLLVLKTTNSAFK
                     GFVRDRYRTLKDTDDRIFATSVTASWDYSSIAADFVAAHEGIRTAMLDVFATHMSYAV
                     QETMYEMGRAALSAAPEISNIRLRLPNKHRIPVNLQPFGLENKNEIFVWTDEPFGDIS
                     AFIERK"
     gene            complement(5157..5672)
                     /gene="uraD"
                     /locus_tag="VLI90_15085"
     CDS             complement(5157..5672)
                     /gene="uraD"
                     /locus_tag="VLI90_15085"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HSV15584.1"
                     /translation="MKTSLAQLNAADCNDFVAVCGPLFEHSPWIAERTWPRRPFATLD
                     ALHAALCETMHAANLDEQVKLIASHPDLVGRLAREGRLTRESTAEQSAAGLAQLSDDE
                     IAAFDRYNNAYRDRFGFPFVICARENKKEAILAAFPVRLRNTREQEIAAALGEIAKIA
                     RLRLLEGVSQT"
     gene            complement(5779..6999)
                     /locus_tag="VLI90_15090"
     CDS             complement(5779..6999)
                     /locus_tag="VLI90_15090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HSV15585.1"
                     /translation="MPINVDRIWRDIEAIAGFNETTPSVGYSRPTFSPAWRQARDYVI
                     AQAEATGCQTRIDAAGNVHARPRDLPSDAPAWLSGSHIDSVPTGGKYDGVAGVVAPLE
                     ILRAAREDAKPIALELIIFAEEEGTTFGLGMLGSRAWVGELTADDLAKVRSTSGQNYL
                     EAGREHGVRPDDLPRERFNRSAYIGLVEIHIEQGPDLWNLRSPIAIVSSIAGRRQYRV
                     AFHGVANHAGATSMRTRKDALAAAAPIIIQVEAMARDLSPQTVATVGRIDCRPNAINV
                     IPERVDFTIDLRSPNDALLAEGHERITKYVMQSAERREMRCELTMTEDQPAMQLDQTI
                     CARLKRAAGEVSMAVSGALHDAAILAPQVPTAMIFVASKDGISHNPEEFSRVEDIAAA
                     ATVLQNGISGEPSP"
     gene            complement(7246..>7529)
                     /locus_tag="VLI90_15095"
     CDS             complement(7246..>7529)
                     /locus_tag="VLI90_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15586.1"
                     /translation="RSVIDVMDENGIEDYLTGRCSRTCEFAVRTRGPRVINENEIRKI
                     FAETEAGVFARDWMQEWSLGMPQLHRLRRSARESDMEKTGAQWRKDFGK"
BASE COUNT         1333 a         2377 c         2369 g         1450 t
ORIGIN      
        1 tttcccgcgc gcttcaactg atcgaatgct tcggcaatct cggtcgggtc catgagcacg
       61 tccggccggt gcaggtgata caggtcgatc gtctcgatgc cgatgcggcg aagcgattgc
      121 tcgcacgacc acacgatgtg cttcgccgag aagtcgtagc gatgcggcgc atccggcgtc
      181 ggatcaccgg cgaatctgat gccgcacttg gtggcgatca cgatgcgctc gcgcatgccc
      241 gacacctcgc gcagcgcctg gcccaggatt tcctcgcaca ccccgcggca ataaatgtcg
      301 gcggtgtcga acagcgtgta tcccgcttcc agcgccgcga cgtgcgcgcg cacgccggcg
      361 gagcgcatct ccgccgtcac tgctttgggt tcccacgtgc cggccgaccg catgttgccg
      421 tacgccagcc gggtgctttt caagccgctc ttgccaatcg tttgcgtatt catgttggac
      481 atcaaaagtt atcggaacct taggaaacgg ccgaaaaaac cggatttctt ttcccatttt
      541 gacggccgat gttattttat tccgcgtcgg cagcctaccc gcgagcggag caacatgaca
      601 accggatcca cggccaccaa gcgttgtatc acctgtggaa aagacgtcac caccatcaag
      661 cggatgaaag attcgcaagg ccgctactgg tgcgttgact gcgggatggc tgatcggcgc
      721 aagaagatga aggcggcctc gaagagcaag tccgtctcca acagcggtgg cggggtgagc
      781 ctgatggaac gcctccgcga ccacaccggc ggcggtggcg ccggaggcac cgacaagggc
      841 cggctggtga agatgctggt ggccgtggcc atcatggccg ccattgccgg ctggcagttc
      901 ctgtcgcatt gaaccaccca tcaaccgcgc caggttcgga gggtactccc gacgaaccgg
      961 atggtttact gagagtgctt cactttgcag tcgtacgctc ccaagcgctt tgcccctcca
     1021 gcgtcactcg attcaccttt cgatccaacc gttcagccgc cgcgttgaaa tcattcgtga
     1081 tccaatccaa ctcagcgccc gtatccaagc tacccttggc gagcgattcg aactccggcc
     1141 gcgccggcgg accagattga ttcatcgcga gaagtgccgt cagaatcgct tcatccgcgc
     1201 ttgctccgcg cagcgcatcc acatcgcaat cgacttcgat gaacgacgcc ggccgacccg
     1261 gcgcaaacgt gccgagttga tccgccaagc cgagcatctc cgccgccttg atcgtcgtcc
     1321 gcacgagcgc ttcgctcggc gttgcccgtc gcgatcgccc cgcgtgaact ttgaggaact
     1381 gcgccatctc gcacaggatc gacgtcgtcg gcgacgcccc cacatcggtg cagatcgcgt
     1441 aaggtatgtt gcgatcgatc acgccgtcca gccgcatgat gcccgagccc agcagcgaat
     1501 tgctcgtggg acaatgcgcg atgaccgcgc cggcggcggc gagttgatcg aactccgcat
     1561 cgcgcatgtg gatgcagtgg gccatgatcg cctcgcgctg cggcagtccg tctcgggcat
     1621 agacgtccgt gtagctggcc gcatcgggat acagcgtccg ttccacaagc tccttttcgc
     1681 gcagctgctc gtttaaatgc gtctgcgtcc gcaacccgaa tcgctccgcc agtgcgacgg
     1741 cccgccggcg cagcggtgaa tccacggcca cagcgaagcg atcggtgacg atcatccgcc
     1801 ggccgaactc gcgcgcgagc gattcaacat cgcggtcgag gtttgtctcg tccgtgcgca
     1861 ggtacgccgg gcaattcatg ttcatgagca ccaagccgac gctccacgtc gccggcagat
     1921 cgtgaagcgc ctgcgcgacc gcaggggcgt ggacggtcat atatgccgcg ccaccgacga
     1981 cgccgtgggc gagcgtgtcc tcgcgaaacg cggcgacggt ttcgatcgta tggacaggct
     2041 cggcacatcg cgcttcgtac ggaaagacgt tgcgattcaa cccggccagt aaccgaccct
     2101 cggcggggca gccgacgacg ccatcgcaga atcgcccgcg gatgggccat tgcgggatgt
     2161 gaatgtgaca gtcgagaaac ggcggaagga taacgccgca gctttggacg ttgcgagccg
     2221 gtgtcgcagg gagatgtgca gcatcgccga tgtacgtgat gcgcccgtgc tcatcccatt
     2281 gcagcgcgcc gttctgatgg aattcgacgg tgccgctcgg caaggggtta aggatcggac
     2341 cgcgcagcgt ggtcgtggag catgacatca ttgcatgaac cagccgattg cggcttcgca
     2401 aacccgatac gctggtctga gaagccgcaa gcggcaaagc gttaacgccg tctttgaggt
     2461 ttcaccaatt ggccgcgcgc ggcattgaca gtcgcgccgt cctgaaagat cgtcatcccg
     2521 cgcaggatcg tccgccgcac gacgccatgg aaacatcggc cgatgtacgg gctgagcttg
     2581 tgccgatcca gcagctgctc gcgcttcagc tcgaaacatc cggtcacatc caccagcgca
     2641 aaatccgcgt cgaatcccgc ttcgatcttg ccctttccgg cgatgcgaaa tcgcgaagcc
     2701 ggcgtcgccg ccgtcacctt cgcgaccagc ggtaacggca gcgccggccc gcggctcagc
     2761 aggatcggca gcgtcgattg cacacccgcc acgccgcccc agactttgaa aaagttggca
     2821 tccgtcttca tccccggtgg cgacggcgaa tggtcggacg cgatgatcgg gatctttccc
     2881 gccgccaagt cctgccaaag ctcgtcgttc tccgccggcg tgcgcagcgg tggagcgcac
     2941 ttggcgctgg cgccgagtcg ttcgacatcg tgccggttca gcgccaggta atgcgggcag
     3001 gtttcgcacg tcacatcgca ttcgccgcgc gccgcggcac ggcggacttg ttcgatcgat
     3061 cgcgaattgc tcacgtgcac gatgtgcagc cggcatccgg tttcttccgc gaggatgatc
     3121 gcgcgctcga ccgcttcgac ttcggcaacg atcggccgcg aatctaggta cgccgcgacc
     3181 gacgtgtggc ccgccgccat cgcctcggcg gcgagcattg acgtcatcgt gtcgctctcg
     3241 gcgtgcaccg caaccggcag gtccagctgc ttcgccttcc gcatcgcgcg gtagagcgtg
     3301 agatcgtcgg tccgcgggaa atcttctatg ccgctattgg acatgaacgc cttaaacccg
     3361 acgacgccgc ggtcggcgag tgcttccatc tgatcgaggt tgcccggcac gatgccgccc
     3421 cagaggccga agtcggcgta gcttcgaccg gtcgcggcgg tgagcttgcc atcgaacgcc
     3481 gcggcgtcga tcaccggcgg cgaggagttc agcggcatgt cgaaaaagca cgtcccgcca
     3541 ccggccgcca gcgccgcgga gccggtgtca aatccttccc aatcggcccg gccgggatcg
     3601 ttgaagtgaa cgtggatgtc gatgaggcct ggaaaaacgt gaaggcccgt ggcgtcgatt
     3661 tcttcgcgcg ccgagccggc gatttgcgat gcgaattcga ccactttgcc gtccgcgacg
     3721 gcgacatcgg cgcgatgaac gccgctcggc gtgacgaggt gaccgtttcg gatgagcagg
     3781 tcgtgattca ccagaggcct cagaggagta cccgctctcc cggtactccg ggagagggct
     3841 ggggtgaggg tgatttggaa tgaccagagc cgctcgcgct tgaaatcacc ctcaccctaa
     3901 ccccctcccg gtgtaccggg agaggggacc agaggaacat caactcccgc ggtacgtgct
     3961 gtacgcccac ggcgacacca gcagcggcac gtgatacgcc gcgttggcgt cgtcgatacg
     4021 gaacagcacg ggcaccgcgt cgagaaatcg ctttgcgtcg ggcgaacctt tggcggtgaa
     4081 ataattgccg acgtgaaaca gcaggcggta atgaccggtg gccatcgcgt cgccgctgag
     4141 cagcggcgca tcgctgcggc cgtcgcgatt ggtgacgaca tccgccagcg gcagcttctt
     4201 gtccgcttcg agccggtgca gctcgatgcg catgccgccg gcgggggttc cgttggcggt
     4261 gtcgagcacg tgcgtggtga gcttgccggg cattgcgatc ttcactttcg ttcgataaat
     4321 gcgctgatat cgccgaacgg ttcatcggtc cacacgaaga tctcgttctt gttttccagt
     4381 ccgaacggct gaaggttgac cgggattcga tgcttgttgg gcaggcgaag gcggatgttg
     4441 ctgatttccg gcgcggcgct gagcgcggcc ctgcccatct cgtacatcgt ttcctgcacc
     4501 gcgtagctca tgtgcgtcgc gaagacgtcg agcatcgcgg tgcggatgcc ttcgtgggcg
     4561 gcgacgaaat ccgcggcgat cgaagaataa tcccacgacg cggtgacgct ggtggcgaag
     4621 atgcgatcgt cggtgtcttt cagcgtgcga tatcgatccc tcacgaaacc tttgaaagcc
     4681 gaatttgtcg tcttcagcac gagcagatcc tgaacgccgc cggcgatcgt cgcggcgcca
     4741 ctgcgtgtgg acgtggcgac ggcggtgtgc agttcggcgc cgccggagac gaacgcgtgg
     4801 tcatgcggct tgccgttggt gttgatccgc gaccagctgc tttggcgcac ggtgacggtc
     4861 gccgtttcga cgtggctgta ggtcttcgtg aaatgctcgg cgagaagaat cgcgaactgt
     4921 tcggcggaat tgaaatcccg ctctttcgcc agcacgtaca ccgtgttttt cattgaatcg
     4981 gttgcgacga tcttgctgtt gtcgccggcc gtgtatgacg ccgcgaaatc acctcgcagt
     5041 tcgacatcga cggaaaattc gaacaggtca tggtgcgccc cgcggcggac gaccttggtc
     5101 agccgaacgt ccgatttgcc gtagtggttg gcggtgagtt tgatggacat attctctcaa
     5161 gtctggctga cgccctcgag caggcggagc cgcgcgatct tcgctatttc gcccagggcg
     5221 gcggcgattt cctgctcccg cgtgtttcgc aaccgcacgg gaaacgccgc aaggatcgcc
     5281 tcttttttgt tttcgcgagc gcagatgacg aacggaaagc caaagcggtc gcgatatgcg
     5341 ttgttgtaac ggtcgaaggc ggcgatttca tcatcgctca attgcgctaa ccccgcggcg
     5401 gattgttcgg cggtcgattc gcgtgtcaag cggccttcgc gcgcgagtcg gccaaccaga
     5461 tcgggatgcg acgcgatcag cttcacctgc tcgtcaaggt tggcggcgtg catcgtctcg
     5521 cacaacgccg cgtgcagcgc atcgagcgtc gcgaacggcc ggcgcggcca ggtgcgctcg
     5581 gcgatccacg gcgaatgctc gaacagcggc ccgcagacgg cgacgaagtc gttgcagtca
     5641 gcggcattga gttgagcgag tgatgtcttc aaagcggttc atcgtgacac gggcctccgg
     5701 cccgtgcttg cgactttcaa attgagaaga atccgctgtt ccaaatatga caccgctcgc
     5761 acgggccgga ggcccgtgtc acggtgaagg ttctccactg ataccgttct gtaggaccgt
     5821 cgccgcggcg gcgatgtctt cgacgcggct gaattcttcg ggattgtgac tgatgccgtc
     5881 cttgctggcg acaaagatca tcgccgtcgg cacctgcggt gcgagaatcg cggcatcgtg
     5941 cagcgcaccg ctgacggcca tcgacacctc gccggccgcc cgcttcaacc gggcgcagat
     6001 cgtttgatcc aactgcatcg ccggctgatc ttcggtcatc gtcaactcac atcgcatctc
     6061 gcggcgctcg gctgattgca tcacatactt cgtgatccgt tcgtgccctt cggccaggag
     6121 cgcatcgttc ggtgaacgca ggtcgatcgt gaaatcgaca cgctcgggga tgacgttgat
     6181 cgcgttcggc cggcagtcga tccggccaac ggtcgccact gtctgcggcg acagatccct
     6241 ggccatcgct tcaacctgga tgatgatcgg cgcggccgcg gccagcgcat ccttgcgcgt
     6301 gcgcatggac gtcgccccgg catgattggc gacgccgtgg aacgcgacgc gatattgccg
     6361 gcggccggcg atggagctga cgatcgcgat gggcgatcgc aggttccaca gatcggggcc
     6421 ctgttcaatg tggatttcga cgaggccgat gtatgccgag cggttgaagc gttcgcgcgg
     6481 cagatcatcg ggtcgaacgc cgtgctcgcg cccggcttcg agatagtttt ggccggacgt
     6541 gcttcgcacc ttcgccagat cgtctgccgt cagttcgccc acccacgccc ggctgccgag
     6601 catgccgagg ccgaaggtgg tgccttcttc ttcggcgaag atgatcagct cgagcgcgat
     6661 gggcttggcg tcttcgcgag cggcgcggag gatttccagc ggcgcgacga cgccggcgac
     6721 gccgtcgtac ttgccaccgg tggggaccga gtcgatgtgc gatccgctga gccacgcggg
     6781 ggcgtccgac ggcagatcgc gcggccgagc gtgcacattc cccgcggcgt cgatgcgagt
     6841 ctggcagccg gtcgcctcgg cctgcgcgat gacgtagtca cgagcctgcc gccacgccgg
     6901 cgagaacgta ggccggctgt agccgacgct cggcgtcgtc tcgttgaagc cggcgatggc
     6961 ttcgatgtcg cgccagattc tgtcgacgtt gatgggcatt cgtttggtcc cctcgcccat
     7021 gtactcatgg gagagggtta gggtgagggt gattcagaca atgaccaatg agcaaaatcc
     7081 caaatcccaa tgaaatcaca agcaccaatg cccatgcgcg agcccacgcg ggaattggtc
     7141 attggagctt caatgggatt tggcgcttgg acattggaca ttctcacatc tgaaatcacc
     7201 ctcaccccaa ccctctcccg gattaccggg cgagggggta agaaatcact tcccaaaatc
     7261 cttccgccac tgcgcgcccg tcttctccat gtcactctcg cgcgcgctcc tgcgcagccg
     7321 gtgcagttgc ggcataccga gcgaccattc ctgcatccag tcgcgggcga acacgccggc
     7381 ttcggtttcg gcgaagatct tgcggatctc gttttcgttg atcacccgtg ggccgcgcgt
     7441 gcgcacggcg aattcgcacg tgcggctgca tcggccggtg agataatctt cgatgccgtt
     7501 ctcgtccatc acgtcgatca cgctgcgca
//
LOCUS       DATIMH010000870         5681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_495692, whole genome shotgun sequence.
ACCESSION   DATIMH010000870 DATIMH010000000
VERSION     DATIMH010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495692"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VLI90_15100"
     CDS             complement(<1..1311)
                     /locus_tag="VLI90_15100"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSV15587.1"
                     /translation="MPSLSRFINMTAAVSTGVLLAGCASSQPKNLTADAFMPKPVVSA
                     TSPDLDRPPAPQVAADLPKTAVALPAPTTEPADRPPALDATPAAEPTLASASASATTL
                     PAESPTTAPVAPDLYMTVGGVVAEVNGTPIYANELLRKLDKILAAKAREMDPASFRIY
                     ARELLKRQREEMIQSEREYAAAERLLTPDDKKLIDQMAMPRFYQQKVIEAGGSVEQAK
                     RKAKAEGDDFDEMLRREHRKQMQELFRERKITPRIQVSADDMREYYRMHIADYTQRAE
                     AQFRVIKIDPRRIGGENARAVALARINAIREKAINGADFASLASAENQDDYLKANAGD
                     PGGWMQRDAYRIDKVDKAVWELQPGQVTPVITDDDAFYIAKLEARKDGRVQPFEDQAV
                     QDKIHSELARRQFVQLLASIDAQLEGESIVRREDALLDTAVDMAM"
     gene            complement(1453..2352)
                     /gene="metF"
                     /locus_tag="VLI90_15105"
     CDS             complement(1453..2352)
                     /gene="metF"
                     /locus_tag="VLI90_15105"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HSV15588.1"
                     /translation="MPEPQDHRFRIDRLLGVGLPTVSFEFFPPKTDAGFQSLFQTIDD
                     LKSIRPSYVSVTYGAGGSTREKTVNLVERIQRELGIRSMAHLTCVGHTATEIGSILND
                     IWKAGIRNVLALRGDPPAGQSQFIATEGGFANADALVKFVAERHPFCIGVAGYPEGHP
                     QCLNKTRDLEHLKRKVDNGANFVVTQLFFDNEDFYRFRDAARAIGIKAPIVAGIMPIT
                     NVAQIKRFIGMCGAKIPNPLLLKLETVEADAEAVHAAGVEYAIQQCNDLIGNGVDGIH
                     FYTLNKSKATVQICKSLNIQHAA"
     gene            complement(2611..3912)
                     /locus_tag="VLI90_15110"
     CDS             complement(2611..3912)
                     /locus_tag="VLI90_15110"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HSV15589.1"
                     /translation="MLRRNGLALVAACAALASAGLVRAADDAAVSVSQPIRMDDTAVP
                     ATDAPAAETPRQPLMWLFDKAGLAKPLDDAGINIYGYVEGSYQHDFSAPPDLPGGPTN
                     FINGRAFDVQSDVPNFNQLDLTVERTVDPTKGKFDIGFRVEQIYGEDAAFIHSNGLTT
                     YAPDKIGLARKPKVQYDLNQAYIDVAIPVGNGLDIRIGKFNTLLGYELISPNANALFS
                     HSFLFTQLPFTHTGVLASYNLTKELSVTGGVTRGWDQALEDVNGSLDATGQIKWASDK
                     VAFYLNGITGPEQPSGASNVWRTVLDVIATYQYSDNLLLAVNGDYGWEPGIDAGKTGQ
                     WYGVAAYAKYKITDMFTLNVRGEWFDDQDGSAPGTFLGAPNQYYEATLGLSITPFPLN
                     NIGKNLVIRPEVRYDYADKPTWDLGTDSNRLTAAVDAYFQF"
     gene            4481..5662
                     /gene="nadA"
                     /locus_tag="VLI90_15115"
     CDS             4481..5662
                     /gene="nadA"
                     /locus_tag="VLI90_15115"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSV15590.1"
                     /translation="MAKVWQQPLPEIYTQLSDPELRIRIEAAREALGSKLAILGHHYQ
                     QDAIIDFADFTGDSFELSRRAAEQKGVEYVVFCGVHFMAESADILTDEKVHVILPDLG
                     AGCSMADMANLDQTIECWEQLKQTCPDQLIVPITYMNSSAAIKAFVGEHGGAVCTSSN
                     CRNVLEWALRGGAAPAALSSGKKVKMLFFPDQHLGRNTAYAMGYPLDKMVVWDPREDL
                     GGNCEAQLNNADFVLWKGHCSVHALFRPEHVDDVRKNHPNMKVIVHPECKWEVVQKAD
                     MAGSTAYIVDQVRKAPPGTEWAIGTEVHLVNRLKQQHPDQRIVVLSDCQCLCTTMYRI
                     DLPHLCWVLENLVDGNVVNEVKVDATTRKWSLVALERMLAIKGTGNPIGKQQPVGTTV
                     D"
BASE COUNT         1035 a         1747 c         1794 g         1105 t
ORIGIN      
        1 catcgccatg tccacggcgg tgtcgagcag ggcatcctct ctgcgcacga tggactcgcc
       61 ttccagctgc gcgtcgatcg acgccagcag ctgcacgaac tgccggcggg ccagctcgga
      121 gtgaatcttg tcctgcaccg cctggtcctc gaacggctgc acccggccgt ccttgcgcgc
      181 ttccaacttc gcgatgtaga acgcgtcgtc atcggtgatg accggcgtga cctgcccggg
      241 ttgcagctcc cagacggcct tgtcgacctt gtcgatgcgg tatgcgtcgc gctgcatcca
      301 cccgccgggg tcaccggcgt tggccttcag gtagtcgtcc tggttctcgg ccgatgccag
      361 cgaggcgaag tcggcgccgt tgattgcttt ttctctgatc gcattaatcc gcgccagcgc
      421 caccgcccgg gcgttttcgc cgccgatccg gcgcgggtcg atcttgatca cgcggaactg
      481 cgcctcggcg cgctgggtgt agtcggcaat gtgcatgcgg taatactcgc gcatgtcgtc
      541 ggcgctgacc tggatccgcg gcgtgatttt gcgctcgcgg aacagctcct gcatctgctt
      601 gcggtgctcg cgccgcagca tctcgtcgaa gtcgtcgcct tcggctttgg ccttgcgctt
      661 ggcctgctcg accgaccccc ccgcttcgat caccttctgc tggtaaaacc gcggcatggc
      721 catctggtcg atcagcttct tgtcgtccgg cgtgagcaag cgctcggcgg cggcgtattc
      781 gcgctcgctc tggatcattt cctcgcgctg ccgcttgagc agctcgcgcg cgtagatgcg
      841 gaacgacgcc gggtccatct cgcgcgcttt ggcggcgagg atcttgtcga gctttcgcag
      901 cagttcgttg gcgtagatcg gcgtgccgtt cacctcggcg acgacgccgc cgacggtcat
      961 gtacagatcc ggtgcgaccg gggcggtcgt gggtgattcg gcgggcagag tggtcgcaga
     1021 cgcggaggcc gacgccagcg tcggctccgc cgcgggggtt gcgtcgagcg cgggcggacg
     1081 atcggcgggc tcagtcgtcg gcgccggcag cgcgacggcg gtcttgggta gatccgcggc
     1141 gacctgcggg gcgggcggac gatccaggtc cggcgaagtc gcgctcacca cgggctttgg
     1201 catgaacgca tcggcggtga gatttttcgg ttgcgaagac gcgcagccgg cgagaagcac
     1261 accggtcgag accgcggcgg tcatattgat gaatctgctc aagctcggca ttcgacgaag
     1321 ctccggttcg gtccccacac gggtccgata agtattatcg accggggcga cattaccaag
     1381 agaggaaaac cgcccggcgc ggtggagttg cagagatcgg ggtcgtagct ccgaacccgc
     1441 cgtggggagg aactatgccg cgtgctggat gttcagggac ttgcagatct gcacggtcgc
     1501 cttgctcttg ttcaatgtat agaagtggat gccgtccacg ccgttgccga tcaggtcgtt
     1561 gcactgctgg atggcgtatt ccacgcccgc ggcgtgcacg gcctcggcgt cggcttcgac
     1621 cgtttcgagc ttcaacaaaa gcggattggg aatcttcgcg ccgcacatgc cgatgaaccg
     1681 cttgatctgg gcgacgttgg tgatcggcat gatgccggcg acgatcgggg ccttgatgcc
     1741 gatcgcgcgg gcggcatctc gaaatcggta gaagtcttcg ttgtcgaaga acagctgtgt
     1801 gaccacgaag ttcgcgccgt tgtccacctt gcgcttgagg tgttccaaat cgcgggtttt
     1861 gttcaggcac tgcggatggc cttcgggata cccggcgacg ccgatgcaga acgggtggcg
     1921 ctcggcgacg aacttgacca gcgcatcggc attggcgaag ccgccttcgg tggcgatgaa
     1981 ttggctttgg ccggcggggg gatcgccgcg aagggccagc acgttgcgga tgccggcttt
     2041 ccagatgtcg ttgaggatgg aaccgatctc ggtcgcggtg tgtccgacgc aagtcaggtg
     2101 ggccatcgag cggatgccga gctcgcgctg gatgcgctcg acgaggttca ccgtcttttc
     2161 gcgcgtgctg ccaccagcgc cgtaggtgac cgagacatag ctgggccgga tggacttgag
     2221 atcgtcgatg gtctggaaga gggactgaaa ccctgcgtcc gtcttgggtg gaaagaattc
     2281 gaacgagacc gtgggcagtc ccacgccaag caggcggtcg atgcggaagc gatggtcttg
     2341 cggttcgggc atatctgtta aatcggcatt ttacgaatca gcgaaagaca atcatcacac
     2401 cggcggcaag gaacattata tcatccgtcc atccggatta acagatgaaa tgttacgttt
     2461 gaaacaaaat gttacgtttc gaggcgttca acgtagtgcg caacgcgtga cgggcaacgc
     2521 gtgacgggca acgcgtgacg cgaaacgcga cagggcgtcg cggggatgcc gcgacgccct
     2581 gttggttatt atttaatggt cagacgcgac ttagaactgg aagtacgcgt ccaccgccgc
     2641 cgtcaggcgg ttgctgtcgg tgccgaggtc ccaggtgggt ttgtcggcgt agtcgtaacg
     2701 gacttcgggg cggatcacga ggttcttgcc gatgttgttc agcgggaatg gcgtgatcga
     2761 aagacccagc gtcgcttcgt agtactggtt cggcgcgccc aggaacgtgc ccggggcgga
     2821 tccgtcctga tcgtcgaacc attcaccgcg aacgttgagg gtgaacatgt cggtgatctt
     2881 gtacttggcg taagcggcca cgccgtacca ctgcccggtc ttgccggcgt cgatgccggg
     2941 ttcccagccg tagtcaccgt tgaccgccag cagcaggttg tcgctgtact gatacgtggc
     3001 aatcacgtcc agcacggtgc gccagacatt gctggcgccc gagggctgct cggggccggt
     3061 gatgccgttg aggtagaagg cgaccttgtc gctcgcccat ttgatctgac cggtggcgtc
     3121 gagcgaacca ttgacgtctt ccagcgcctg atcccacccg cgggtgacac cgccggtgac
     3181 gctcagttcc ttcgtcaggt tgtacgaagc cagcacgccg gtgtgcgtga agggcaactg
     3241 ggtgaacagg aagctgtggc tgaacagagc gttggcgttc gggctgatca actcatagcc
     3301 aagcaacgtg ttgaacttac cgatgcggat gtccagaccg ttgccgaccg ggatcgccac
     3361 gtcgatgtac gcctggttca ggtcatactg aaccttgggc ttgcgggcga ggccgatctt
     3421 gtccggggcg taggtcgtca ggccattcga gtgaatgaac gcggcgtctt caccgtagat
     3481 ctgttcgacg cggaaaccga tgtcgaactt acccttggtg gggtcaaccg tgcgctcgac
     3541 ggtgaggtcg agctggttga agttcgggac gtccgactgc acgtcgaagg cgcgaccgtt
     3601 gatgaagttc gtcggaccac ccggcaggtc cgggggcgcg ctgaagtcgt gctgatacga
     3661 gccttcgaca tagccgtaga tgttgatccc ggcatcatcg aggggcttgg ccagaccggc
     3721 cttgtcgaac aaccacatga gcggttgacg gggcgtttcg gccgccgggg cgtccgtggc
     3781 ggggacggcg gtatcgtcca tccgaatcgg ttgactcacc gacacggcgg catcgtcggc
     3841 ggcccggacc aggcccgcgg aggccagggc ggcgcacgcc gccaccaggg ccagaccatt
     3901 cctacgcaac atagctgctc cttcccaaaa gggattccca aactgggggt tgttgcccgc
     3961 aacgggcggg tcatgcccgc taccgggggt gccccagcgt cggccagtac cgcgccgggg
     4021 aacatctgaa acgagagact gcttccacac tcaaacccgt cgcgcccttt acaaacgagg
     4081 tgccatcgac ggcggcgttc gatcgaacgc cgtgcatttc gtccaaaccc tcgcgttctc
     4141 atgccacgcg ccgggtccgt ccttttctcc gcacacccgt tggctgtcaa cacccgccta
     4201 acgcatgggc agatatcgaa cattcgctgc cgttttgttg aaaaagtctg caaaaccggc
     4261 aaactcatcg acagcttcgg gccgttttaa cggctaatcg cctatgttgg ttcccataac
     4321 ttcacctgtg gataaccttt acatcgcctc gtttttcctg tttttcaaac agccgccgag
     4381 actgaaacgc ctgggcttat tggaacaagc ctacgattca ggcgtaaata tcgtccgacg
     4441 cccgctgatt gattgaacgt ttcgcccggt gagacaccct atggcaaagg tctggcaaca
     4501 gccgcttcct gagatctaca cccaactgtc cgaccccgag ctgcgcatcc gcatcgaggc
     4561 ggctcgcgaa gcgctcggtt cgaaactggc catcctcggt caccactatc agcaggacgc
     4621 catcatcgac ttcgccgact tcaccggcga cagcttcgaa ctgtcgcgcc gcgccgccga
     4681 gcagaagggc gtcgagtacg tcgtcttttg cggcgtgcat ttcatggccg aatccgcaga
     4741 catcctcacc gatgaaaaag tccacgtgat cctgccggac ctgggcgcgg gctgctcgat
     4801 ggccgacatg gccaacctcg atcagacgat cgagtgctgg gaacaattga agcaaacctg
     4861 cccggaccag ctgatcgtcc ccatcactta catgaacagc agcgccgcca tcaaagcttt
     4921 cgtcggcgaa cacggcggcg ccgtctgcac aagctcgaat tgtcgcaacg tcttagagtg
     4981 ggccctgcgc ggcggcgccg ctccagcggc actgtcttcc ggcaagaagg tcaaaatgct
     5041 cttcttcccc gaccaacacc tcggaagaaa caccgcctac gccatggggt accccctcga
     5101 caaaatggtc gtctgggacc cgcgcgaaga cttaggcggc aactgcgaag cgcaactaaa
     5161 caacgccgac ttcgtcctct ggaaaggcca ctgctccgtc cacgccctgt tccgccccga
     5221 gcacgtcgac gacgtgcgca agaatcaccc caacatgaag gtgatcgtcc accccgagtg
     5281 caaatgggaa gtcgtccaga aggccgacat ggccggcagc accgcctaca tcgtcgacca
     5341 ggtccgcaag gcccctcccg gcacagagtg ggccatcggc accgaggtcc acctcgtcaa
     5401 ccgcctcaag cagcagcacc ctgaccagcg aatcgtcgtc ctgagcgact gccagtgcct
     5461 ctgcaccacg atgtaccgca tcgacctccc ccacctctgc tgggtgctgg agaacctggt
     5521 cgatggcaac gtcgtgaacg aagtgaaggt cgacgccacc acccgcaaat ggtcactcgt
     5581 cgcgctggaa cgcatgctgg cgatcaaggg aactggcaac ccgatcggca aacagcagcc
     5641 ggtggggacg acggtggatt gaacctgtct cgtgccggcg g
//
LOCUS       DATIMH010000871         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_204846, whole genome shotgun sequence.
ACCESSION   DATIMH010000871 DATIMH010000000
VERSION     DATIMH010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204846"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VLI90_15120"
     CDS             <1..513
                     /locus_tag="VLI90_15120"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV15591.1"
                     /translation="ASAYNNLLTNAGGVDLGDTDGALVLDYAGNADPAAAVRSILANN
                     TGQIRSSTMTAGHAIGYVDDATASKVSVRVTLLGDANLDQSVDVADLGELATSYGATT
                     GGTWAAGDFNYDNRVDVADLGMLATNYGQTSIAATGAFIHADVTTVPEPAMLALFAVA
                     AVLLGRDRRR"
     gene            1023..3263
                     /locus_tag="VLI90_15125"
     CDS             1023..3263
                     /locus_tag="VLI90_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xyloglucanase"
                     /protein_id="HSV15592.1"
                     /translation="MRLIACCCFWLLIGLLAPARAQQVASAPYEWKNVKVVAGGYVPG
                     VVFSRVARGLAYCRTDIGGCYRWDEAQQRWLPLMDSIGTSSYFGGESIAPDPVDANIV
                     YVAAGMYSTEDAAILRSGNRGDTWDVIPVPFRMGGNEDGRGMGERLAVDPNDNRVLYF
                     GSRHDGLWTSGDSASTWQKVQSFPLPGLGNPSPARVAHAGLSFVVFDRTSGARGSRSR
                     TIFVGSADPGEQHLFRSDDAGQSWHAVEGGPNRRMLPAKAEMDDAGQLYVTFGTSIGP
                     NDVADGAVWKLDVRAGQWTDITPIKSPDRSAGGYCGLSIDRQHRGTVAVTTIDRWHDG
                     DTIFRSEDGGATWVDALAHSQRDISLSPYLLWGKGESRPGWWMSALAIDPFNSDHAAY
                     ATGATIYGTGEFTRADGARTTRWNVWADGMEETAVLALISPPSGAPLVSGFGDLCGFA
                     HHDLSASPADGMFTNPLLSTVTQVEYAGAQPAVIVRRGSPDDRGRSIGFSEDGGATWH
                     PLLAPRTSSGDDGRRRHGGAMSVIVGADGQTLVFTTSAGPSITRDRGGDWNASNGLPP
                     GARPVADRVDPMRFYAIDFKSASFYISADGAATFTKLISQGLPGDLTQERAGGGDGNW
                     PLQATPGRAGDLWFVSRRGLFHSQDGGGTFVNVRGGIDVEFLSFGKAPAGRDEPELFA
                     IGTAGLLRAIWRSDDSGASWLRVNDDRHQYGTRFRCITGDPRVFGRVYVGTDGRGIVY
                     GDATAH"
BASE COUNT          568 a          989 c         1169 g          587 t
ORIGIN      
        1 gccagcgcgt acaacaatct tctgaccaac gccggcggcg ttgatttagg cgacaccgat
       61 ggcgcgctcg tcctggacta cgcgggtaac gcggatcccg cggctgccgt gcggtcgatc
      121 ctcgcgaaca acactggcca aatccgaagc agcacgatga cggccggtca tgcgatcggc
      181 tacgtcgatg acgccaccgc gagcaaggtc agcgtccgcg tgacgctgct gggcgatgcg
      241 aacctggatc agtccgtgga cgtggcggat ctgggcgagc tggcgacgag ctacggcgcg
      301 accaccggcg gcacctgggc ggcgggggac ttcaattacg acaatcgtgt ggacgtcgcc
      361 gacctgggca tgctggcgac gaactatggg caaacgtcca tcgccgcaac cggtgcattc
      421 attcatgccg acgtcacgac cgtgcctgaa cccgcgatgc tcgcactgtt cgccgttgcc
      481 gctgtattgc tcggccgcga tcgtcgtcga tgacgtcgcg ccgggacctt gaataaaatg
      541 tttgacgtaa gccaaagcgg ctgtcagaat cgattgtgat cataactggc gggcagatgc
      601 aggatgtgaa tttccaagcg tgatttgaag cggtaccgac ggtatcgctt ttctttttgc
      661 gtgaaggcgc aaacgtttgc gcgaacctgg gagcgtcaat tgaaggcggg acttcgcacc
      721 gaaaccggac acagttacct ctggagcctg ctcgacaact tcgaacgggc tgaaggtatc
      781 gcaaggggtg taactcgtga ttgtggcgaa ggccttttct ggtcccctct cccgcgagta
      841 cgcgggagag gggaccggag ggacactcgc cacaattagg agttacaccc tatcgcaagc
      901 gcttcggcat cacgtacgtg gactacacga cgctgcggcg gattccgaag ctgagcgcga
      961 aattttatgc cgaggtgatt cagaagaact cggtggtgtg aggaagggcg cgcggtgacg
     1021 tcatgcgttt gatcgcttgc tgctgtttct ggctgctcat cggtctgctc gcccctgcgc
     1081 gcgcgcagca ggtcgcgtcg gcaccctacg agtggaagaa cgtgaaggtg gtggccggcg
     1141 gatatgtgcc gggcgtcgtg ttcagccggg tagcgcgcgg gctggcgtac tgccgcaccg
     1201 atatcggcgg gtgttaccgc tgggacgagg cgcagcagcg gtggttgccg ctgatggatt
     1261 cgatcggcac gtcgagctac ttcggcgggg agagcatcgc gccggacccg gtggatgcga
     1321 atatcgtcta cgtcgcggcg gggatgtact ccaccgaaga cgcggccatc ctgcgctcgg
     1381 gcaaccgggg tgacacgtgg gacgtgattc cggttccgtt ccgcatgggt ggcaacgaag
     1441 acggccgcgg gatgggggaa cgcctggcgg tggatccgaa cgacaaccgg gtgctttact
     1501 tcggctcgcg ccatgacggg ctgtggacga gcggcgactc cgcttcgact tggcaaaagg
     1561 tccagagttt tccgctgccc gggctgggaa atccatcgcc ggcgcgcgtg gcgcacgcgg
     1621 ggttgagctt tgtcgtgttc gatcggacca gcggtgcgcg cgggtcgcgc agccgaacga
     1681 tcttcgttgg atcggccgac cccggtgagc agcatctgtt ccgcagcgac gatgccgggc
     1741 aaagctggca cgccgtcgag ggcggaccga atcggcggat gctgccggcc aaggcggaga
     1801 tggatgacgc gggacagctg tacgtcacct tcggcacgtc gatcggtccg aacgatgtcg
     1861 ccgacggcgc ggtgtggaaa ctggacgttc gcgccggcca atggaccgac atcacaccga
     1921 tcaagtcacc ggatcgatcc gccggtggat attgcgggtt gagcatcgac cgccagcacc
     1981 ggggcacggt ggccgtgacg acgatcgatc gctggcacga cggcgacacg atttttcgca
     2041 gcgaagacgg cggggcgact tgggtggatg cgctggcgca cagccagcgc gatatctcgc
     2101 tgtcgccgta cctgctttgg ggcaagggtg aatcgcggcc gggttggtgg atgagcgcgt
     2161 tggcgatcga cccgttcaat tccgaccacg ccgcctatgc cacgggcgcg acgatctacg
     2221 gcaccggcga gttcacccgc gccgacggcg cgcgaacgac gcggtggaac gtgtgggcgg
     2281 atggcatgga agagacggcg gtgctcgccc tgatcagccc accgtcgggc gcgccgctgg
     2341 tgagcggctt tggtgacctg tgcgggttcg cgcaccacga tctgagcgcg tcgccggccg
     2401 atgggatgtt cacaaacccg ctgctgtcca cggtgacgca ggtggaatac gctggcgcgc
     2461 aaccggcggt gatcgtccgg cgtggatcac cggacgaccg cgggcgctcg ataggctttt
     2521 ccgaagacgg cggggcgacc tggcacccgc tgctcgcgcc gcgcacaagt tcgggcgacg
     2581 acggccgccg gcggcatggc ggggcgatga gcgtcatcgt tggcgctgat ggtcaaacgc
     2641 tcgtcttcac gacgagcgcc ggtccatcga tcacgcgcga ccggggtggc gactggaacg
     2701 cctcgaacgg gctgccgccg ggcgcgcggc cggtggccga tcgtgtcgat ccgatgcgct
     2761 tctacgccat cgacttcaag tcggcgtcct tctacatcag cgctgatggg gcggcgacgt
     2821 tcacaaagtt gatttcgcaa ggcttgccgg gcgacctcac gcaagaacgc gccggcgggg
     2881 gtgatgggaa ttggccgctg caggcgacgc cggggcgcgc gggcgacctg tggttcgtca
     2941 gccggcgcgg gctctttcat tcgcaggatg gcggcgggac tttcgtgaac gttcgcgggg
     3001 ggatcgacgt cgagttctta agcttcggta aagccccggc ggggcgtgat gagccggagc
     3061 tgttcgccat cggaactgcc ggattgcttc gcgccatctg gcgctccgac gacagcggtg
     3121 catcgtggct tcgcgtgaac gacgaccggc atcaatacgg cacgcgcttc cgctgcatca
     3181 ccggcgaccc gcgcgtcttc gggcgcgtct acgtcggcac cgatggccgc gggatcgtct
     3241 atggtgacgc gacggcgcat tagcctcgcg caatccgacg cagcgctaga ctgatcagtc
     3301 ctcccggcaa aag
//
LOCUS       DATIMH010000872         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4247899, whole genome shotgun sequence.
ACCESSION   DATIMH010000872 DATIMH010000000
VERSION     DATIMH010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4247899"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..819
                     /locus_tag="VLI90_15130"
     CDS             244..819
                     /locus_tag="VLI90_15130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV15593.1"
                     /translation="MAAASSHLDDDFALMQAIAAGDRAALERLYDRHSPLVLAVSRRV
                     LKDAAEAEDVLTTVFFEVWSRADRFDASRGSPLSYLTTLARSRAIDRRRSRAARPVAL
                     SSENSAAENAAAMASTAPTPLQSSDINEQRQRIRAALAELEPAQREAIECSFYEGLSH
                     SEIAEKLQKPLGTVKTYIRQGLIRLRDRIRT"
     gene            867..1655
                     /locus_tag="VLI90_15135"
     CDS             867..1655
                     /locus_tag="VLI90_15135"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HSV15594.1"
                     /translation="MLGYLTGTLEPAEEVELRQHLASSCPACAGALAEAQATLASLPL
                     ALDSVAPSPLAKARLMQRVDQWAASATPERVPYSPPLHFMRIAIPAAIAACIAVMLTH
                     ALVTRQLRPQILRGQLAERLVQQQNLQIDSLLTSLKDQKHYVDMLRSPDLKVVQLNGA
                     AQPNALARIMWDQKSGQWLLLTEGLAPMPPNKVYELWFINTDSKKIAAGTFNVDDKGQ
                     ATMLTKVPANAGSFLVAAITDEPGPVVDPTGTIQLVSRPLGNVQ"
     gene            2163..>2684
                     /locus_tag="VLI90_15140"
     CDS             2163..>2684
                     /locus_tag="VLI90_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15595.1"
                     /translation="MIMRGDISMRVAFIIVVLGLTVAAPAERQWVGGSGVNSDWWWYG
                     NSDTVHGDYNDSNSPDAAGRAAHDSIANDPNAGLNHPGTPGNDEIAQASAAMDAAQAR
                     VERAFESSPEWTNAVADSESGKRAYHKARAEVVKALSERADYTSAAEASAAADRTVES
                     SRATTTATQAEMHA"
BASE COUNT          502 a          888 c          846 g          448 t
ORIGIN      
        1 agttgctccc accccgctac caaggtggca tccatgccgg cctccgtttg tgtctcttgc
       61 aaaacacaat tggaaggtcg gcgtggtctt ttgtggagcc ccgatcttca taaccaaggc
      121 caaaagcggc taaagttcac aatacgtgaa taacgtgatt acgtgacgcg tgtgcggcgc
      181 gctctcgctt cttgtcacgt attcacgtat tcaagtcgct acgcccgaac cagggaatca
      241 gggttggccg ccgcgtcttc acatctcgac gatgacttcg cgctgatgca ggcgatcgcc
      301 gccggcgatc gtgcggcgct ggagcgcctt tacgatcggc acagtccact cgtcttggcc
      361 gtctcccgcc gcgtgctgaa ggatgccgcc gaggccgagg acgtgctcac cacggtcttc
      421 ttcgaagtct ggtcccgcgc cgatcgattc gacgcgtcgc gcggcagtcc actgagctat
      481 ctgacgacgc tggcccgcag ccgggcgatc gaccgtcggc gctcgcgcgc cgcccggccg
      541 gtcgcgctca gttccgaaaa tagcgccgcc gagaacgccg ccgcgatggc gtccaccgcc
      601 cccacgccgc tgcaatcatc tgacatcaac gagcagcgcc agcgcatccg cgcggcgctg
      661 gccgagttgg aaccggcgca gcgcgaggcg atcgaatgtt cgttctacga aggcctcagc
      721 cacagcgaga tcgccgaaaa actgcaaaaa ccgctgggaa cggtcaaaac ctatattcgg
      781 caaggactca tccgtttgcg cgatcggatt cgtacttaat gaaatggagc cgttgaactt
      841 tgacgtgtga tgagcgacga aacctgatgc tcggctactt gactggcacg ctggagccgg
      901 ccgaggaggt tgagctgcgc cagcacctgg ccagcagttg ccccgcgtgc gcgggcgcgc
      961 tggccgaagc gcaggcgacg ctggcgtcgc tgccgttggc gctcgattcg gtcgcgccgt
     1021 cgccgttggc caaggcgcgc ttgatgcagc gtgttgatca gtgggccgcc tcggcaacgc
     1081 ccgagcgcgt gccctacagt ccgccgcttc acttcatgcg catcgccatc cccgccgcca
     1141 tcgcggcgtg catcgcggtc atgctcacgc acgcgctggt cacgcggcaa ctccgcccgc
     1201 agatcctccg cggccagctg gccgagcgcc tcgtccagca acagaacctg cagatcgatt
     1261 cgctgctgac cagcctgaag gaccagaagc attacgtcga catgctgcgc agcccggacc
     1321 tgaaggtcgt gcagttaaat ggcgccgccc agcccaacgc gctggcccgc atcatgtggg
     1381 accagaagag cggccaatgg ctgctgctga ccgaaggcct ggcgccgatg ccgccgaaca
     1441 aggtgtacga actgtggttc atcaacaccg actcgaagaa gatcgccgcc gggacgttca
     1501 acgtggacga caagggacaa gcgacgatgc tgacgaaggt gccggccaac gcggggtcgt
     1561 ttctggttgc cgcgatcacg gatgagccgg ggccggtcgt ggacccgacg gggacgattc
     1621 agctggtgag cagaccgctc ggtaatgtgc agtgagggtg gtcgctggcg ccggaagagt
     1681 cccaccgcag ggccatgggc ttttctcagc gcgcatcgac gagatcaaag cccacggctc
     1741 tgccgtggga ctctttttcc gaacttgttc aaaccgccta tgatgcgggc gcaaacattc
     1801 gcgcgcccgc atcaccagca ccgacgacac aacgagcttt ttgccgcgcc tcacgggcgt
     1861 cggccgcgac gccgctcgcg ccaccttgcg cgccgactgg tcacaatcta agccaggcga
     1921 cgcgctggtc tgtctcgccg cgattatcgc atccctgttc gtcgccatcg cgctgacgcg
     1981 gcgacttctc ctaacgacaa acccattccc gatgagggtt cacctacgtg aagaatgcgg
     2041 ctccggtccc ctcgcccggt acgccgggag agggggaaag agccacgcgc gcgatcgccc
     2101 tgcgttcccg atcaggcact gggaaattga ttgcacatcg ccatgccccg gcggataatt
     2161 gcatgatcat gcgcggggac atttcaatgc gcgtcgcgtt cattatcgtc gtccttggcc
     2221 tcacggtcgc cgcgccggcg gagcggcagt gggtcggcgg cagcggtgtg aacagcgact
     2281 ggtggtggta cggcaacagc gacaccgttc acggcgatta caacgactcc aattcccccg
     2341 acgccgccgg gcgggcggcg cacgactcga ttgcgaacga tcccaacgcg gggttgaacc
     2401 atcctggcac gccgggcaat gacgaaatcg cccaagcctc ggccgcgatg gacgcggcgc
     2461 aggcgcgggt cgagcgcgcg ttcgaatcgt cgccggaatg gaccaacgcc gttgcggact
     2521 ccgagagcgg caagcgcgcg taccacaagg cgcgggcaga ggtcgtcaaa gcgctatcag
     2581 agcgagcgga ttacacatcg gcggccgagg ccagcgccgc ggcggatcga acggtggaat
     2641 catcgcgtgc caccaccacc gcaacgcagg cggagatgca cgcc
//
LOCUS       DATIMH010000873         1960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2226845, whole genome shotgun sequence.
ACCESSION   DATIMH010000873 DATIMH010000000
VERSION     DATIMH010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2226845"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VLI90_15145"
     CDS             complement(<1..185)
                     /locus_tag="VLI90_15145"
                     /inference="COORDINATES: protein motif:HMM:NF013813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HSV15596.1"
                     /translation="MKFTKMHGIGNDYVYVNGFAEAVDDPAAVAQKISDRHFGIGADG
                     LILIVPSTNGADVRMRM"
     gene            complement(245..1117)
                     /locus_tag="VLI90_15150"
     CDS             complement(245..1117)
                     /locus_tag="VLI90_15150"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSV15597.1"
                     /translation="MRARREHVEVGGESSFFCRRARGRGWPFDWHFHPEYELTLIVHG
                     RGQRFVGDDIAEFRDGDLVLLGGNLPHTWHSSSRMRMPSEAVVVQFAADCFGDQFFDR
                     PETRHIGRLLRQASAGLQFRGRSASAAAQQMLGIADQPPLRRLATLLEILDLLAGCRA
                     ACALATPRFRPVSRPSDQRRIHRALQFVHDRLTTPSMSMQDAAAHTHLSRSAFSRFFQ
                     RATGKRFARYVNERRVGRACQLLAETDRNIAVIALDCGFGNLSNFNRRFKEVRGVVPR
                     VYRRKFQSDDPAAA"
     gene            1329..>1960
                     /locus_tag="VLI90_15155"
     CDS             1329..>1960
                     /locus_tag="VLI90_15155"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HSV15598.1"
                     /translation="MTTTTLDAQLHIDSPYALSPAQIAQFRNNGFVKLKDVLSAQMLA
                     HYGREISQKVKELNTLTLPMEQRTTYQKAFLQITNLWTRSDVVKEFVMSKRLGRLAAE
                     LMGCRGVRIYHDQALYKEPSGGITPWHADQFYWPLETNHTVTAWIPLQATPLELGPLA
                     FSSGSHRFNSIGREIEISDESEKKLTKALLESGLPLDETPFDLGEVSFHLG"
BASE COUNT          404 a          597 c          612 g          347 t
ORIGIN      
        1 aacatgcgca tgcgcacgtc cgcgccgttg gtggacggca cgatcaggat caacccatcc
       61 gcgccgatac cgaagtggcg atcggaaatt ttttgagcca ccgccgccgg gtcatcgacg
      121 gcttcggcga agccgttgac gtaaacgtag tcgttaccga tgccgtgcat cttggtgaac
      181 ttcataacga tggataatag gacaaccccg cgtgcctggc gaggcttgcg cggagcaacg
      241 accgctatgc ggcggcggga tcgtctgatt gaaatttccg tcgataaacg cgcggcacga
      301 ccccccgaac ttccttaaag cggcgattga agttggacag gttgccgaag ccgcagtcca
      361 gcgcgatcac cgcgatgttc cggtccgtct ctgccaatag ttggcatgcc cggcccacgc
      421 ggcgttcgtt cacataccgc gcgaaccgtt tgccggtggc gcgctggaag aagcggctga
      481 atgccgaacg gctgagatgc gtgtgggcgg cggcgtcttg catcgacatc gatggcgtcg
      541 tcaatcggtc atggacgaac tggagcgcgc gatggattcg ccgctgatcg ctggggcgtg
      601 acaccggccg gaagcgcggc gtcgccagcg cgcacgcggc tcgacagccg gcgagcaggt
      661 cgagaatctc cagcaacgtc gccaatcgcc gcagcggtgg ttgatccgcg atgccaagca
      721 tctgctgcgc cgcagcgctg gcggacctcc cgcgaaactg aagccccgcc gacgcctgac
      781 gcagcaatcg gccgatgtgc cgggtttcag gccgatcgaa aaactgatcg ccgaagcagt
      841 cggcggcaaa ctgcacgacg accgcttcgg atggcatccg catccgcgag cttgaatgcc
      901 aggtgtgcgg cagatttcca ccgagcagga ccagatcacc gtcgcgaaac tcggcgatgt
      961 cgtcgcccac gaaccgctgt ccgcggccgt ggacaatcaa cgtcaattcg tattcgggat
     1021 ggaagtgcca atcgaaaggc caacccctgc cgcgcgcccg gcggcagaag aaggaggatt
     1081 caccgccaac ctcaacatgt tcccgacggg cccgcatttg atatcattct accatcagta
     1141 ccgccgcagt tcctgttgga cgacaattaa gtatcagttt gtgcccagat gatcgcagac
     1201 ggcagccgcg tcactcgtca caatccccgc gtcacctttg aacgtgcgac cggtgcgagt
     1261 atcgccgctg gctccgacac ggttcacagt tctcgcggct ttgattgttc attgaacgga
     1321 gattcgccat gaccacgacc acccttgatg cgcagttgca tatcgattcg ccctacgcgc
     1381 tttcgccggc gcagatcgcg cagtttcgca ataatgggtt cgtcaagctg aaagacgtgc
     1441 tgtccgcgca gatgctggcg cattacgggc gagagatcag ccagaaggtg aaggagctga
     1501 acacgctgac gctgccgatg gagcagcgaa caacctacca gaaggcattt ttgcagatca
     1561 cgaacctgtg gacgcgcagc gatgtggtca aagagttcgt gatgagcaag cggctcggtc
     1621 gcctggcggc ggagctgatg ggctgccgtg gtgtgcggat ctatcacgac caggcgctct
     1681 acaaggaacc gtccggcggg atcacaccgt ggcacgccga ccagttctac tggccgctgg
     1741 agacgaatca cacggtgacg gcgtggatcc cgctgcaggc gacgccgctg gaactgggcc
     1801 cgctcgcgtt cagcagcggc agccaccgct tcaacagcat cgggcgcgaa atcgagatct
     1861 ccgacgagtc ggagaagaaa ctgaccaagg cgctgctgga gagcgggctg ccgctggatg
     1921 agacgccgtt cgatctgggg gaagtatcct tccatctagg
//
LOCUS       DATIMH010000874         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3096434, whole genome shotgun sequence.
ACCESSION   DATIMH010000874 DATIMH010000000
VERSION     DATIMH010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3096434"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1488
                     /locus_tag="VLI90_15160"
                     /pseudo
     CDS             4..1488
                     /locus_tag="VLI90_15160"
                     /inference="COORDINATES: protein motif:HMM:NF042838.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6785 family protein"
     gene            1573..1956
                     /locus_tag="VLI90_15165"
     CDS             1573..1956
                     /locus_tag="VLI90_15165"
                     /inference="COORDINATES: protein motif:HMM:NF042608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6784 domain-containing protein"
                     /protein_id="HSV15599.1"
                     /translation="MASAVDQFAQGRFQPKQHNPYFHLGLGFVATVLLQVASLRWAAW
                     PLLPVGYVASYGAFVENAWFSIFVGWLAQVLIVRFGGASLFQKAKPFFIGIIFGEGLA
                     AGIWLIVNAIVVLNGGTGQSVRFLL"
     gene            complement(2183..>2811)
                     /locus_tag="VLI90_15170"
     CDS             complement(2183..>2811)
                     /locus_tag="VLI90_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15600.1"
                     /translation="SYLGGALQGYETDPLSIGIAYTTTPDEPRPWTRLVSNPVLAPSD
                     ADVRPFERKTLYKSNIIRDESRSLGAPFVMFYNGKQEGPGAERIGIALSNDMTHWRRF
                     GAGPVIDNGPRGISGDPQIVRMGDLWVMFYFGHVWKPKAFDTFACSYDLVNWTKWNGP
                     HLVEPSEPWDATFAHKPWVIRWEGTVYHFYCAVGNEGRAIAVASSSLS"
BASE COUNT          432 a          945 c          853 g          581 t
ORIGIN      
        1 agcatccttc tcggcacgtt gtccgtcatc gccgtctgca cgatcacgcc gctcagcgat
       61 ctcaccgtga acgacacgag cctggccgct ggttacatgc cgctggcagc cgtgctgatc
      121 ctgttcgtcc tcgtcgtcgg catcaacggt tcgctgcacc gctggttccc gcgccgcgcg
      181 ctgcgcagcg gtgaattggc cgtcatcgtc ctcatgacgc tcgtcgcctg cagcctgccg
      241 aactgggggc tgatgcgatt cttcatcccc acgcccatgg cgccgttcca cgtcggcgcg
      301 ggcgattagc aatattggaa ggcattcctc ggcatggacc tgccgcggtg gctgttcccg
      361 gtcgaggaca tcaaggccgg ccgcaccagc ccgatcgcgc tgtggtttta caacaaggtg
      421 cccgaaggcg aagcgattcc ctatcgcgcg tggctcgtgc cgctgctgac gtggggcatc
      481 ttcgccggcg cgatgctggc gacgctcgcg gccatcgcgc ggttgatcat cgaccaatgg
      541 gcgatcaacg aacgcctgcc gtttccaatc gtgcaggtgc acgccgcgct gatcgaacaa
      601 cccgtaccgg ggcgtgcgct caactcgatg ttccgcagcc gcgcgctttg gattggcctg
      661 ctcggggtgc tggcgatcca cctgctctcc tgcctgaacg cgtattggcc gcggcatttc
      721 ccggcgatcc cgctgcggtt cgacctcact aacatctttt ccgaagaacc gttctcgtac
      781 cttcggccga agctgaaaaa agcgctgctc tcgttcaccg tcgtcggcgt cacgttcttc
      841 atccgctcgc gcgcggcgtt cagcctgtgg gcgatgttca tcctggtgaa cctcgtggac
      901 atgcagcagg gcatgcggca atctgagatt cccaacgccg cctggcagga ccagcacctg
      961 ggcgcgtgcg tcgcgttcgt cggtgggatc ttgtggatcg gccggcatca ctggatgcgc
     1021 gtgctgcgca gcgcgttctt cgcccgcggg accgatcgaa cctacaccgc cactttctgg
     1081 gtcgccgtgg ccggcatcgt ggtcatgctc ggctggttgt ggttcctcgg cgtgcacttc
     1141 tgggtggcgg ctttgatcgt gctcttcatc gtggtggcgc acctggtcgt cgcccgcgtc
     1201 gtcgccgaaa cgggattgcc cttctatcgc tgcggcatcg ccgcgtcgca gatctacggc
     1261 aacttctccg cccggctttt cacgatgcgc gacatcttct tcggctcgat cttcaacgtg
     1321 ctcggcccgc tgacgacgcg cgacggcttg acgaccttcg ccaccacagg cctgggcgtc
     1381 tgcaacgccg ccgacgtcga atccggccgg cgccggcggc tgggtggtgt gatggcgctg
     1441 gcgctgatcg tcggcttcgt cgccgcgtcg ttcaccacgc tgcattgcca gtattcatac
     1501 ccgacgcccg gatcgcccga agccaaaccg caacggaact atttcgcggc cgaatacatc
     1561 ccccggcggg acgtggcatc ggcggtcgat caattcgcgc agggacggtt tcaacccaag
     1621 cagcacaatc cgtatttcca cctcgggctc ggattcgtcg cgaccgtgct gctgcaggtc
     1681 gcgtcgctgc gctgggccgc ctggccgctg ctgccggtcg gttacgtcgc cagctacggc
     1741 gcgttcgtcg aaaacgcctg gttcagcatc ttcgtcggct ggctggccca ggtcctgatc
     1801 gtccgcttcg gcggagcgag cttgttccaa aaggccaagc ccttcttcat cggcatcatc
     1861 ttcggcgaag gcctggccgc gggaatctgg ttgattgtaa acgctatcgt cgtgctaaac
     1921 ggcggcacgg ggcagagcgt gcggtttttg ctgtgatttt ccccgccttg tcctgagcgc
     1981 agcacgtact cgtgcgcagt cgaaggatat agctggaagc gggtgaacta tgattctgcg
     2041 caaatggtgg ggcagtcgct ttctgcttcg ggcaccgagc atttgatccg cgaaaccgca
     2101 agcgactcct caatgttcgc gcaaacggcg cttcgcccac ttctgtctat atccttcgac
     2161 tccggcgggg taccgcctgc gctcaggata aacttgatga ggcgacagca atcgcccgcc
     2221 cctcattccc caccgcgcaa tagaagtgat acaccgttcc ctcccaccga atcacccacg
     2281 gcttgtgggc gaacgtcgca tcccacggct cgctcggctc gaccagatgc ggcccattcc
     2341 acttcgtcca gttcaccagg tcatacgaac acgcgaacgt gtcgaacgcc ttcggcttcc
     2401 acacgtggcc gaagtaaaac atcacccaca ggtcgcccat gcggacgatc tgtggatcgc
     2461 cggagatccc gcgcgggccg ttgtcgatga ccgggccggc gccgaagcgt cgccagtgcg
     2521 tcatgtcgtt cgacagcgcg atgccgatcc gctcggcgcc ggggccttcc tgcttgccgt
     2581 tgtagaacat cacgaacggc gccccgagcg accgcgattc atcccggatg atgttgctct
     2641 tgtagagcgt cttgcgctcg aagggccgaa cgtcagcgtc cgacggcgcg agcaccgggt
     2701 tgctgaccag ccgcgtccat ggccgcggtt catccggcgt cgtcgtgtag gcgatgccga
     2761 tcgatagcgg atcggtctca tacccctgca gcgcgccgcc gaggtagctg a
//
LOCUS       DATIMH010000875         1840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3957686, whole genome shotgun sequence.
ACCESSION   DATIMH010000875 DATIMH010000000
VERSION     DATIMH010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3957686"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1766)
                     /locus_tag="VLI90_15175"
     CDS             complement(81..1766)
                     /locus_tag="VLI90_15175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV15601.1"
                     /translation="MPLNPLDYPAALMQPRYVSDASAWCGHVPFAFALVQMLRPQSIV
                     ELGTHKGDSYMAFCQAVAAMQLPTRCSAIDTWRGDVQAGGYGEEVLAQLRQAHDPLYA
                     NFSRLIRSSFDQAATMFAPGSIDLLHIDGLHTYDAVKHDYETWRPKLSRRGVVLFHDV
                     AVRSGDFGVWRLWEELAPRHPHFLFEHAHGLGVLAVGDDVPAPVLNFLADARQHADRV
                     RAFFARLGDSVEYARFHRQLVPWLAQQQVILNAWRQRVGLAIDSAAADINAASTDTIN
                     FVARSTSQLDAALVEDLRLRQELQREKTQPPPAASAITPRLVSVIICSITDAKFNAAS
                     AMYQRLLAAVPHEIIRISDARSMTEGYNRGIGRSTGDVLIFSHDDVEFLNADFAQRLL
                     RHLGSFDLVGVAGTNLVCGPAWVSAGPPYLFGQVTHPDPRGFTVSFFGAPRAVITDIQ
                     ALDGLFFALHRRVVEKVRFDESLPGFHYYDLDFTYSAFLAGFKLAVVNDIHAIHHSQG
                     DFGQRWAEQAGRFNEKWAGRLPPRPNRPLSLSGVTVKTREEIWEVVHAAHLEI"
BASE COUNT          337 a          545 c          639 g          319 t
ORIGIN      
        1 tcgacggcgc caccgccgcc gaatttctgc gcacgctcaa agccctgctc gaagtgccgg
       61 ggatgatgct ggtgtgaacc ttaaatctcc agatgcgccg cgtgcacgac ttcccagatt
      121 tcctctcgcg ttttcacggt cactccgctc aggctcagcg ggcggttggg gcgcggtggc
      181 agtcggccgg cccacttttc gttgaaccgg ccggcttgct ccgcccaacg ttggccgaag
      241 tcgccttgag aatggtggat cgcgtggatg tcgttcacga cggccaattt gaaaccggcg
      301 aggaacgcgg aataggtgaa gtcgagatcg tagtagtgga agccgggcag cgattcgtcg
      361 aagcgcacct tctcgacaac ccggcggtgc agggcgaaga acaggccgtc cagtgcctgg
      421 atgtccgtga tgaccgcgcg cggcgcgccg aagaacgaga cggtgaaccc gcgcgggtca
      481 gggtgcgtga cttgtccgaa caggtacggc gggccagctg acacccacgc ggggccgcag
      541 actaaattag tgccggcgac gccgacgagg tcgaagctcc ccaggtgccg cagcaggcgc
      601 tgggcgaagt cggcgttgag aaattcgacg tcgtcgtgcg agaagatcag gacgtcaccg
      661 gtgctgcgac cgatcccgcg gttgtagcct tcagtcatgc tgcgggcatc gctgatgcgg
      721 atgatctcgt gcggcaccgc ggccagcagc cgctgataca tcgcgctggc ggcgttgaat
      781 ttagcgtcgg tgatgctgca gatgatgacc gagaccagac gtggcgtgat ggccgaagcg
      841 gcgggaggtg gttgcgtctt ctcgcgttgc aattcttgtc ggaggcgcag gtcttcgacg
      901 agcgctgcat cgagctggct tgtcgagcgg gcgacgaaat tgatcgtatc ggttgacgcg
      961 gcgttgatgt cggccgccgc ggaatcgatc gccaggccca cgcgttgccg ccaggcgttg
     1021 aggatgacct gctgctgcgc cagccagggc acaagctggc ggtggaagcg ggcatattcg
     1081 actgaatcac cgagacgcgc gaagaatgcg cgaacgcgat cggcgtgctg ccgggcgtcg
     1141 gccaggaagt tcagtaccgg cgcgggcaca tcatcgccga cggcgagcac gcccagcccg
     1201 tgggcgtgtt cgaacagaaa gtgcggatgt cgcggagcaa gctcttccca cagccgccag
     1261 acgccgaaat ctccgctgcg cacggccacg tcgtgaaaca gcacgacgcc gcggcggctg
     1321 agcttcggcc gccaggtttc gtagtcgtgt ttcacggcgt cgtacgtgtg caggccgtcg
     1381 atgtgcagca ggtcgatcga tcccggcgcg aacattgtcg cggcctgatc gaacgaggat
     1441 cgaatgagcc gggagaagtt cgcgtacagc ggatcgtgcg cctggcgcag ctgggcgagg
     1501 acctcttcac cgtagccccc ggcttgaaca tcgccgcgcc aggtatcgat ggccgagcat
     1561 cgcgtcggca gctgcatcgc cgccaccgcc tggcagaagg ccatatatga atcgcccttg
     1621 tgggtgccga gttcgacgat ggactgcggg cgcagcatct gcacgagcgc gaatgcgaat
     1681 ggcacgtgcc cgcaccacgc cgaggcgtcg ctgacgtatc gcggctgcat cagtgcggcg
     1741 gggtaatcga gcgggttcaa gggcatagag gcctaaagat atcgcaatcg cggcgttccg
     1801 cgtagcgttg aggatcgaat gcagccgtgc ggaattacct
//
LOCUS       DATIMH010000876         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4250407, whole genome shotgun sequence.
ACCESSION   DATIMH010000876 DATIMH010000000
VERSION     DATIMH010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4250407"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VLI90_15180"
     CDS             <1..812
                     /locus_tag="VLI90_15180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HSV15602.1"
                     /translation="SRLLAGVLDPNALSGLYEHRCAAGFQGDVAGVRLPRDHYAAFVE
                     LHIEQGPLLERAGVPLGIVTAIAAPATWHITIEGEGGHAGAVLMPDRHDAFCAAAEVV
                     LAVERCANSTAAIDTVGTIGVCNVFPGAVNSIPSGVRLEMDVRDIDLARRDEVLRKIE
                     QCCVDIARRRGVQIRKNVINADAPATCDPAVVNVLADAADSNGLAYQKMVSRAYHDSL
                     FMARIAPTAMLFIPCRAGISHRPDEYASPEGIAAGALVLAEALATLADATA"
     gene            complement(802..>1554)
                     /locus_tag="VLI90_15185"
     CDS             complement(802..>1554)
                     /locus_tag="VLI90_15185"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSV15603.1"
                     /translation="AEFINRLGQNLVRNSDAKVPFTFQVVEDEIPNAFALPGGFIFVN
                     TGLIKLASEEDELAGAMAHEIAHVAARHMTCQATKGQIANIASIPASILLGGGLGGYA
                     ARQAMGVGAQTAFLKLSRGAEAEADYLGTQYMYAAGYDPTGAISIFEKIESLQKKQPG
                     TLGRVFSTHPMDADRIDKTQKEIQKILPDKPEYVVSTSEYASVRERLIAQGARRKGNA
                     QDGRPRLKVGSRPDSQDEKDERPTIKRRDLMQ"
BASE COUNT          261 a          517 c          463 g          313 t
ORIGIN      
        1 gcagccgcct tctggcgggc gttctcgatc ctaacgcgct atccggcctt tacgagcacc
       61 ggtgcgccgc gggatttcag ggagacgttg ccggcgtgcg cctcccgcgc gatcactatg
      121 ccgccttcgt cgagttgcat atcgagcagg ggcctttact ggaacgcgcc ggcgtaccgc
      181 tcggaatcgt aaccgcgatc gccgcgccgg ccacatggca catcaccatc gagggcgagg
      241 gcggccatgc cggcgccgtc ctgatgccgg atcgtcacga cgccttctgc gccgccgccg
      301 aagtcgtctt agccgtcgag cggtgcgcca attcgaccgc cgcaatcgat acggtcggca
      361 caatcggggt ctgcaacgtc tttccggggg cggtcaatag catcccaagt ggggtccgcc
      421 tcgagatgga cgtgcgcgac atcgacctcg cccgccgcga cgaggtcctc cggaaaatcg
      481 agcagtgctg cgtcgacatt gcgcggcgac gcggcgttca aatccgcaaa aacgtgatta
      541 atgccgacgc cccggcgacc tgtgatcccg ctgtcgtaaa cgtcctcgcg gatgcagccg
      601 attccaacgg gctcgcgtat cagaagatgg tcagccgggc ctatcacgac tcgctgttca
      661 tggcgcgcat cgcgccaacc gcgatgttgt tcatcccctg ccgtgctgga attagccacc
      721 gcccggacga atatgcctcg ccggaaggga tcgcggcggg cgccctggtg ttggccgaag
      781 cgttggcaac actagccgac gctactgcat gagatcccgg cgtttaatcg tcgggcgctc
      841 atccttctcg tcctgcgaat ccggccgcga tccgaccttc agccggggtc gtccatcctg
      901 tgcgttgcct ttccggcggg ctccctgcgc gatcaaacgc tcccggacgc ttgcatattc
      961 cgaggtgctg acgacgtact ccggcttgtc gggcagaatt ttctgaattt ccttctgcgt
     1021 cttgtcgatc cggtccgcat ccatggggtg agtcgagaaa acccgcccca gggtgcctgg
     1081 ctgcttcttc tgcagcgatt cgatcttctc gaaaatgctg attgccccag tcggatcgta
     1141 tccggcggca tacatatact gcgtcccgag ataatcggct tccgcctcag cgccgcgact
     1201 cagcttcaga aacgctgtct gcgcgccgac tcccatggcc tgccgcgccg catatccgcc
     1261 caaaccgcca ccgagcagga tgctcgccgg gatgcttgcg atgtttgcga tctgtccttt
     1321 ggtcgcctgg caggtcatgt gacgggccgc gacgtgcgca atttcgtgag ccatcgcgcc
     1381 ggccagttcg tcttcttcgc tcgccagctt aatgaggccg gtattcacga aaatgaaccc
     1441 gccgggcaac gcaaacgcgt tcgggatttc gtcctccacg acctggaacg tgaagggcac
     1501 cttcgcatcg gagttgcgca cgaggttctg accgagccgg ttgataaact cagc
//
LOCUS       DATIMH010000877         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5698192, whole genome shotgun sequence.
ACCESSION   DATIMH010000877 DATIMH010000000
VERSION     DATIMH010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5698192"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(737..1270)
                     /locus_tag="VLI90_15190"
     CDS             complement(737..1270)
                     /locus_tag="VLI90_15190"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HSV15604.1"
                     /translation="MAEGAHILPNLPKIAATQRTALPYEPVCAVFRRYLHDQKLKFTP
                     ERAMMLDAVLRKNKLFEPEQLVEDLKGLGHRVSRATVYRTLNHLQDAGIVKQVFFDNR
                     QSYYEVIVGRETHDYLICVATGKVIEFSSERLRKLRDEICREHGFQPLSHQFHIFGLS
                     PEGQQRAGSDNAGPKKD"
     gene            complement(1284..2642)
                     /locus_tag="VLI90_15195"
     CDS             complement(1284..2642)
                     /locus_tag="VLI90_15195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HSV15605.1"
                     /translation="MYRFTTWWISIAVALVMGALLARNMRHAISISGSGDLTTVYAAT
                     RVWMHGHDPYDQSQVRREWYAHGGVKAVEPDADHTPSVYPPSTFIVMAPLAAFPWPTA
                     RLIWALLDLGMIAAIAAALPALAGVRWRDPRALALVAAVIALGPLRNGAMGGQPAIVV
                     TGLIVLAALGAQRGWQWTPGVLLAIACAVKPPLAGPFLVYYALRGQWRLALSGAIVAV
                     GLALVGVLRLDMNGVAWLPGLRANLHAAEAAGAINDPTAWSIKRFEMVNLQVLLHTLT
                     DNSHIVALLVRALVGLAAIAFAMLVLQRSRDRRRELAELSVVAALALLPIYHRWYDAG
                     VLVFVLTWGMAALPSLWRAQAIIAMVVVSASFVISPALWLARTLPHAAKPPFRYWRGV
                     EQLVLPVQVWAIVLLAAMLLYVLSFGSKFTPTRLRDDLGADDDDITREREPAFAGTVV
                     GD"
BASE COUNT          528 a          907 c          848 g          468 t
ORIGIN      
        1 tgcggctgga cgatctgcaa gcccccgcgg ggaagggtgc tgagcacgac cgcttcggcg
       61 aaggcgagtt ccttcgccag cgagtcgagc acctgccggt gcgacgggtg ttcaccgcgg
      121 gaggcttgtt ctgcccgtga cgtgtttgaa tgcgctgttt gggccatggt ttcgggggac
      181 aaggttgtgt caatcgtcat gatgacggta atacgacagc cgccatcaaa ggttggatcg
      241 gttcgatcgc tttatgacat ttttcatttc gcgtggcgga cgctacatat gttcgccaag
      301 cgctgacatg cgggcgattt acggccgcga cagaaccgaa ggttatatcg acttacaacc
      361 gccccgtccg ctaacaatta ggtaccaagc aattctaggc ccgttgcaag gtttcttcaa
      421 atcatcacac attggcaaat acagctgtat ttgatgccac cgtgcaacat gcggcacaac
      481 cggcctcggc gtcaacggtg gaactgtcgc agaaggccgg gattatttgc ctcagtcacg
      541 cccggcgggc tatggtcctg cggttgaagc cggtggcacg ccggttgttt taccgctggg
      601 cgaggagccg cctgatgact ttgattaaca cccgtttcct gagcaacccg ttgtactggg
      661 acaagaaggt caagatcgcc atcctgaccc tggcgacgtt catggccctg tgctgattcg
      721 gacctgccgg cccgcgtcag tccttcttcg gccccgcgtt atcggacccg gctctctgct
      781 ggccttcggg gctcaatcca aagatgtgaa actgatgtga cagcggctga aagccgtgct
      841 cgcggcaaat ctcgtcgcgc agcttgcgca accgttcgct gctgaattcg atcaccttgc
      901 cggtcgccac gcagatcagg tagtcgtgcg tctcccgccc gacgatcacc tcgtagtacg
      961 actggcgatt gtcgaagaag acctgcttga cgatgcccgc atcctgcagg tggttcagcg
     1021 tgcgatagac cgtcgcccgg ctgacgcggt ggcccaggcc tttcagatct tccaccagct
     1081 gctccggctc gaacagcttg ttcttgcgca gcaccgcgtc gagcatcatc gcccgctcgg
     1141 gcgtgaactt cagcttctga tcgtgcaggt atcggcggaa gaccgcgcag accggttcgt
     1201 acggcaacgc ggtgcgctgc gtggccgcga tcttcggcaa gttcggcagg atgtgcgcgc
     1261 cctcggccat gcggtagccg agtctagtcc cccaccacgg tcccggcaaa cgccggttcg
     1321 cgctcgcggg ttatatcgtc gtcgtccgca ccgagatcgt cgcgcaggcg cgtgggtgtg
     1381 aacttgctgc cgaagctcaa cacgtacagc agcatcgcgg ccagcaggac gatcgcccac
     1441 acctgcacgg gcaagaccag ttgttcgacg ccgcgccaat aacggaacgg cggcttggct
     1501 gcgtgcggga gcgtgcgggc cagccacagc gccggcgaga tgacgaacga cgcggagacg
     1561 accaccatcg cgatgatcgc ctgcgcgcgc cacaggctgg gcagcgctgc catgccccag
     1621 gtcagcacga agaccagcac gccggcgtcg taccaccgat gatagatcgg cagcagcgcc
     1681 agggcggcga cgacggacag ctcggccagc tcccgccgcc gatcgcgcga gcgctgcaac
     1741 acgagcatcg cgaacgcgat cgccgccaat ccaacaagcg cgcgcacgag aagggcgacg
     1801 atgtgcgaat tgtccgtcag cgtatgcagc agcacctgca ggttgaccat ttcaaatcgc
     1861 ttgatgctcc acgcggtggg atcgttgata gcccccgcgg cctcggcggc gtgcaggttc
     1921 gcgcgcagcc ccggcagcca cgccacgcca ttcatgtcca gtcgcagcac acccaccaag
     1981 gccaggccca ccgcgacgat ggcgccgctc agcgccaacc gccactgccc gcgcagcgcg
     2041 tagtacacca agaacggccc ggccaacggt ggtttgacgg cgcacgcgat ggcaagcaag
     2101 acgccggggg tccattgcca accgcgttgc gcgcccagcg ctgccagcac aatcaggccc
     2161 gtgacgacga tcgccggttg cccgcccatt gcgccgttgc gcagcggtcc cagtgcgatg
     2221 accgccgcca ccagcgccag cgcccgcgga tcgcgccagc gcacccccgc cagcgccggt
     2281 agcgcagcgg cgatggcggc aatcatcccc agatcgagca gcgcccagat cagtcgcgcc
     2341 gtcggccacg gaaacgccgc cagcggcgcc atcacgatga acgtgctcgg cggataaacg
     2401 gacggtgtgt gatcggcgtc cggttcgacg gccttcaccc cgccatgagc ataccactcg
     2461 cgccgaacct gcgactggtc gtacggatcg tgcccatgca tccacacgcg cgtggccgcg
     2521 tagaccgttg tgagatcccc gctgccgctg atcgagattg cgtggcgcat gttgcgcgcc
     2581 aacagcgcgc ccatcaccag cgcaacggcg atggaaatcc accacgtcgt gaaacgatac
     2641 atgccaaatc caatgtcgcg aaaaacaagc acgcccggag gcgtcccact caggattgcg
     2701 gagggaatga gacctcaatt acggaagaaa gtaaagcggc ggggtacgct g
//
LOCUS       DATIMH010000878         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1947326, whole genome shotgun sequence.
ACCESSION   DATIMH010000878 DATIMH010000000
VERSION     DATIMH010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947326"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="VLI90_15200"
     CDS             complement(<1..1275)
                     /locus_tag="VLI90_15200"
                     /inference="COORDINATES: protein motif:HMM:NF021205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HSV15606.1"
                     /translation="MRREGWVWLAACALLACAGSASAAGCTLAKYGTLSVEMIGERPT
                     TLVKVNGAVTRFAIDTGAWFNFMSRANAEALGLKPVPAPFGFRMGGIGGSASAEVATI
                     KDLGFLDTDLHDVDFVVGGSDAGEGSLGANLLDFADLEIDLAQGKVTLFKPDGCRKSA
                     LAYWVKGSGTYNVADLHPSVHLNDLRSFVDVTINGKTVRALLDSGAVATLLDRRAAEH
                     VGIDLHGPRVKPGGRIRGIGAKTYASWIVPIDTFSVGTETIQHSEMLVMDGNFGDGSH
                     DMILGVDFMLAHHMYIANSQRKLYFTYNGGRVFSLDTASIGAHESAAAAANNGEPKTT
                     SDYALRGRARLARGELANAHADLDAAIRLDPGNADDHLIRARILLAQKQPDAALDDLD
                     KALSLDPKNLDARLLRARLRYEKKDEAGAVADL"
     gene            complement(1340..2215)
                     /locus_tag="VLI90_15205"
     CDS             complement(1340..2215)
                     /locus_tag="VLI90_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HSV15607.1"
                     /translation="MSQLDANVYYVPHSSRWPIIAVIGMFLFMVGAAVWLNGYDGPGQ
                     IILTIGALTIIGMMFGWFSNVIRESMKGIFNGQVDTSFRMGMMWFIVSEVFFFAAFFG
                     GLFYIRTFVVPWLGGAGDKGILTNGFIWQGFSAAWPTNGPDSVGGSFIATSPWGIPLI
                     NTLLLLSSSITVTIAHHALRAGNRKRLIGFLGLTIVLGAIFLGLQAHEYWESYVHLNM
                     TLHSGIYGATFYILTGFHGLHVTLGTIMLIVMWLRCARGHFTPQHHFAFEAVSWYWHF
                     VDVIWLCLFLFVYIL"
     gene            2294..2596
                     /locus_tag="VLI90_15210"
     CDS             2294..2596
                     /locus_tag="VLI90_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15608.1"
                     /translation="MPLQQAGDEKRDRDRRDDPRGTPRARLPLRKLVRRRLFRIDLLV
                     CSKHALWNSNGAAFVENKAMDGRGGVAIADDCSRTVVSLRSDQGRSHRSMTAKIND"
     gene            complement(2586..>3104)
                     /locus_tag="VLI90_15215"
     CDS             complement(2586..>3104)
                     /locus_tag="VLI90_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HSV15609.1"
                     /translation="SGVMMALVPADFQYNQTYFIVAHFHYVLAAGALFAIIAGVYYWL
                     PKWSGHMYSNFWTKLHFWSSVIGINLLFFPQHFLGLAGMPRRIPDYNVAFADWNMVSS
                     IGGFWFGATQLIFVGVVIHCVFFSKQKATSRVWEHAYGLEWTLPSPAPHHSFTVPPVI
                     DDTQLAHGDISH"
BASE COUNT          604 a          952 c         1010 g          538 t
ORIGIN      
        1 gagatccgcc acggcgcccg cttcgtcttt tttttcgtaa cgcagccggg cacgcagcag
       61 ccgtgcgtcg aggtttttcg gatcgaggct cagcgccttg tccaggtcgt ccagcgcggc
      121 atcgggctgt ttctgggcga gcaggatgcg ggcccgtatc aaatgatcat ccgcattgcc
      181 tggatccagc cggatcgccg catccaggtc ggcgtgcgcg ttcgcgagtt cgccgcgcgc
      241 caggcgcgcc cggccgcgca gcgcataatc gctcgtcgtc ttcggttcgc cgttgttcgc
      301 cgcggcggcg gccgactcgt gcgcgccgat cgaagcggtg tccagcgaaa acacgcggcc
      361 gccgttgtac gtgaaataga gtttgcgctg gctgttggcg atgtacatgt gatgggccag
      421 catgaaatcg acgcccagga tcatgtcgtg cgaaccatcg ccgaagttgc cgtccatcac
      481 cagcatttcg ctgtgctgga tcgtttcggt gccgaccgag aacgtgtcga tgggtacgat
      541 ccacgacgca tacgttttgg cgccgatgcc gcggatgcgg ccgcccggct tcacgcgcgg
      601 cccgtgcaga tcgatgccca cgtgttcggc ggcccgccgg tcaagcaggg tggcgacggc
      661 accgctgtcc agcaaggcgc gcaccgtttt gccgttgatc gtgacgtcga cgaaactgcg
      721 caagtcattc aaatggacgg aggggtgcag gtcggcaacg ttgtaggtgc cgctgccctt
      781 gacccaataa gccagcgccg acttgcggca gccatccggt ttgaacagcg tcaccttgcc
      841 ctgcgcgagg tcgatttcga gatccgcgaa atcgagcagg ttggcgccca gcgatccttc
      901 gcccgcatcc gatccgccga ccacgaaatc gacgtcgtgc aaatcggtat cgagaaagcc
      961 gaggtccttg atcgtggcaa cttccgcaga ggcagagccg ccgatcccgc ccatgcggaa
     1021 cccgaatggc gccgggaccg gcttcagtcc cagcgcctcg gcgttggcgc gcgacatgaa
     1081 gttgaaccag gcgccggtgt cgatcgcgaa gcgcgtgacc gcgccattga ccttgacgag
     1141 cgtggtcggt ctttcgccga tcatctcgac ggacagcgtc ccgtatttgg ccagcgtgca
     1201 gcctgcggcc gacgcggagc ctgcgcaggc aagcaatgcg caggcggcga gccagaccca
     1261 tccttcacga cgcatcgcgt tctcggagct gaagccgccg attcgctctt tgcagcgccg
     1321 caaacgcggc tttacggcac tagaggatgt acacgaacag gaacaggcac agccagatca
     1381 cgtccacgaa gtgccagtac cacgacaccg cttcgaacgc gaagtggtgc tgcggcgtga
     1441 agtggccgcg cgcgcagcgc agccacatca cgatcagcat gatggtgccg agcgtgacgt
     1501 gcaggccgtg gaaaccggtg aggatataga acgtcgcgcc gtagatgccg ctgtgcagcg
     1561 tcatgttgag gtggacgtag gattcccagt actcgtgcgc ctgcagaccg aggaagatcg
     1621 cgcccagcac gatggtgagg cccaggaatc cgatcaggcg cttgcggttg ccggcgcgca
     1681 atgcgtggtg cgcgatcgtc acggtgatgg acgaggacag cagcagcagc gtgttgatca
     1741 gcgggatgcc ccacggcgag gtggcaatga acgagccgcc caccgagtcc gggccgttgg
     1801 tcggccaggc cgcggagaag ccctgccaga tgaagccgtt ggtgaggatg cccttgtcgc
     1861 cggcgccgcc cagccacggc accacgaagg tccggatgta gaacaggccg ccgaagaacg
     1921 cggcgaagaa gaacacctcg gagacgatga accacatcat ccccatccgg aacgaggtat
     1981 cgacctggcc gttgaagatg cccttcatcg actcgcggat cacgttgctg aaccagccga
     2041 acatcatgcc gatgatggtc agcgcgccga tcgtcaggat gatctggccg ggcccgtcgt
     2101 aaccgttcag ccacaccgcc gccccgacca tgaacaggaa catgccgatc accgcgatga
     2161 tcggccagcg gctgctgtgc ggaacgtaat agacgttggc gtcgagttgg ctcatgtcat
     2221 ggattccggt tatggctggc gtaacggtgt gacccggtca acgtgtgggg acccaattgg
     2281 ggatgcccgc gaagtgcccc tgcagcaagc tggcgacgaa aaacgcgatc gcgatcgccg
     2341 cgatgatcca cgcggtacgc cgcgcgcgcg ccttccgctg cgcaagctcg tccggcgccg
     2401 gctgtttcgc atcgatttgt tggtctgttc gaagcatgct ttgtggaatt caaacggagc
     2461 cgcttttgtt gaaaacaagg ccatggatgg ccgaggtggt gttgctattg cggatgactg
     2521 ttcgcgtacc gtcgtctcat tgcgcagcga tcagggacga tcgcacagat caatgaccgc
     2581 aaaaatcaat gactgatatc cccgtgcgcg agctgggtgt cgtcgatcac cggcggcacc
     2641 gtgaagctgt gatgcggcgc gggcgacggc agcgtccact ccaggccgta ggcgtgttcc
     2701 cacacccgcg acgtcgcctt ctgcttcgag aagaacacgc agtggatcac cacgcccacg
     2761 aagatcaact gcgtggcgcc gaaccagaag ccgccgatcg aggacaccat gttccagtcc
     2821 gcgaacgcga cgttgtagtc ggggatgcgg cgcggcatgc cggcgaggcc gaggaagtgc
     2881 tgcgggaaga acagcaggtt gatgccgatc acgctggacc agaagtgcag cttcgtccag
     2941 aaattggaat acatgtggcc cgaccacttg ggcagccagt agtacacgcc ggcgatgatc
     3001 gcgaacaatg cgccggccgc cagcacgtaa tggaagtgcg cgacgatgaa gtaggtctgg
     3061 ttgtactgga agtcggccgg aaccagcgcc atcatcacgc cgga
//
LOCUS       DATIMH010000879         5867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4834728, whole genome shotgun sequence.
ACCESSION   DATIMH010000879 DATIMH010000000
VERSION     DATIMH010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4834728"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VLI90_15220"
     CDS             <1..1135
                     /locus_tag="VLI90_15220"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15610.1"
                     /translation="RIGDRSTNVDTMTETSRATSDPVESARDAGLVYVNRDGPGITRK
                     RRGKSFEYFDADGKRITDPDELFRIRSLVVPPAWTYVWICPNWRGHIQAVGTDARGRT
                     QYRYHPKWREVRDESKYERMIEFAKVLPKIRARVRRDLRKPGLSREKVLATVVKLLET
                     TLIRVGNGEYAKNNKSYGLTTIRNKHVEVHGSKIHFEFRGKSGVEHAIDIDDPRLAKV
                     VRACQDLPEQELFEYIDEADGSRHDITSSDVNAYLKQITGQDFTAKDFRTWAGTVLAA
                     MALQEFERFDSQAQAKRNVVAAIESVAKKLGNTKAVCRKCYIHPAVIDTYMEGELARS
                     LKRRVEQKLKRSLGALAPEEAAVLALLQQRLAKASKLIGKKAA"
     gene            1269..1625
                     /locus_tag="VLI90_15225"
     CDS             1269..1625
                     /locus_tag="VLI90_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HSV15611.1"
                     /translation="MAWLFLLLAGACEMIWPLGFKYTHGFKTHYPFIALTMAMMTLSF
                     WLMAQATAKGIPVGTAYAVWTGMGATGTAVLGMVWFKEPRDAARIACLLMIVVGVVGL
                     KWLSPVAAVATTAASR"
     gene            1654..2163
                     /locus_tag="VLI90_15230"
     CDS             1654..2163
                     /locus_tag="VLI90_15230"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HSV15612.1"
                     /translation="MGKRSAKAVALIDVEREIVVTRKVLALACVAFGALAFAAAPASQ
                     PQTVHLVVHVADVRNQNGQLIFGVFAQPDAFPNIQSKSVNWQVKPASGDGVFTCDLSP
                     GVYAASVLHDENGNGDMDRNFAGVPTEGYGVTNNPKPKYRQATFKEATFTLPAGGAEL
                     TISIQYKYY"
     gene            complement(2227..2367)
                     /locus_tag="VLI90_15235"
     CDS             complement(2227..2367)
                     /locus_tag="VLI90_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15613.1"
                     /translation="MDCLEHVVEFKVPAEKVGELARFDGSVIDDGAHWFTGGSRPIFA
                     YR"
     gene            complement(2440..2637)
                     /locus_tag="VLI90_15240"
     CDS             complement(2440..2637)
                     /locus_tag="VLI90_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15614.1"
                     /translation="MLPATGFTFAQERAPQPSHQRAAADRQQMSAEQMVAQWPEDCQK
                     AFKQTVAKYGQPNGVTPTLLI"
     gene            complement(2737..4023)
                     /locus_tag="VLI90_15245"
     CDS             complement(2737..4023)
                     /locus_tag="VLI90_15245"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HSV15615.1"
                     /translation="MLSTVVYTESNNPAAGQNAVLAFTRNANGTLSELSSSPFLTGGT
                     GIANPTAALGPDDSDQEVELSADHRFLLAVNQGSNSITVFKIRSDGSLSLVPGAPFSS
                     NGVQPVSLGVDGDKTYVTNRGDEIQGQTGTVSGNYTGFLMHSDGSLTPIANSTITLPV
                     GLSPAQSLISRTGRFVFGDNFTPPPLLNVTDANTIETLRVGSNGSLKALGAVGASVTP
                     PLVLGLAQHPTANILYAGLTGANEVGVFTYDSAGTVTFRGPIADQGMAACWLVVSADG
                     KYLYVANSASDDVGVYSLSDPLHPVQIQEFSLAGPRHLPGATANQTVDFQMTLDPTGR
                     LLYVVNHETAPPPGFAGGNQLHVLRVDEDGALSERKTSPLIFPVSQVPATAHPQGIAA
                     ISLSHAQQQPAFSPRPIRDGDEDDDDHRLHHHKRRD"
     gene            4338..4940
                     /locus_tag="VLI90_15250"
     CDS             4338..4940
                     /locus_tag="VLI90_15250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV15616.1"
                     /translation="MSQEEVMIDQASSDRAVQWLDDHGDALYAYAVLRVRQSQDAEDL
                     VQETLLAAIRARDAFDGDASERTWLISILKHKIADHLRRALRDSGTGHTDGDAAVKAV
                     EPFDKRGVWTVSVKRWPGTPSELLEQAEFRDVLARCLAKLPARMAQLFWLRAAEGKRT
                     AAVCEELEISTDNAWTLLHRARSRLRQCLASNWFGRGDGR"
     gene            4937..5272
                     /locus_tag="VLI90_15255"
     CDS             4937..5272
                     /locus_tag="VLI90_15255"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSV15617.1"
                     /translation="MMRLLRDLRLLNLNCREATRLVSDALDRNLRVFEQAGLSAHLAL
                     CKNCRHFRSNLKFLEELIDAADLVSFSVSTDALPPEAKSRIGRGIDQLTRTSGDASGG
                     GGGQITGSD"
     gene            complement(5588..>5867)
                     /locus_tag="VLI90_15260"
     CDS             complement(5588..>5867)
                     /locus_tag="VLI90_15260"
                     /inference="COORDINATES: protein motif:HMM:NF025862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 C-terminal
                     domain-containing protein"
                     /protein_id="HSV15618.1"
                     /translation="PGTVIGEVAGFARRKGDTWWIGVVNGATERELPISLDFLKTAQS
                     SVLLCDVPGKDDALDRQEKPLGPGDTLTLKLRPGGGFVARIGPANHRP"
BASE COUNT         1146 a         1718 c         1858 g         1145 t
ORIGIN      
        1 tcgaataggc gatcgctcca ctaacgtcga cacgatgacc gaaacatcgc gcgccacatc
       61 agaccccgtc gaatctgccc gcgacgcggg gcttgtttac gtcaaccgcg atggccccgg
      121 catcacccgc aaacgtcgcg gcaaatcctt cgaatatttc gacgcagatg gcaagcgcat
      181 cacggaccct gacgaactgt tccgcatccg ctcgctggta gtcccgccgg cgtggaccta
      241 tgtgtggatc tgccccaatt ggcgagggca catccaagcg gtcggcacgg atgcccgcgg
      301 gcggacgcag tatcgctatc accccaagtg gcgcgaggtg cgcgatgaat cgaagtacga
      361 acgcatgatc gaattcgcca aggtgctgcc gaagatccgc gcgcgcgtgc gtcgcgacct
      421 gcgcaagccg ggattgtcgc gcgagaaggt gctggccacg gtcgtcaagc tgctggagac
      481 gacgctcatc cgcgtgggga acggcgagta cgcgaagaac aacaagtcct acggactgac
      541 gacaatccgc aacaagcacg tcgaagtgca cggctcaaag atccacttcg aattccgcgg
      601 caagagcggc gtggaacacg ctatcgacat cgacgatcca cgattggcca aggtcgtgcg
      661 cgcgtgccag gatctgccag agcaggagct gttcgaatac atcgacgagg cggacggctc
      721 gcggcatgac atcacctcat ctgacgtcaa cgcatacctg aagcaaatca ccggccagga
      781 tttcaccgcc aaggatttcc gcacgtgggc cggcaccgtg ctggcggcga tggcgctgca
      841 ggagttcgag cgcttcgact cacaagcgca ggcgaagcgc aacgtggtgg cggccatcga
      901 atcagtcgcg aaaaagctcg gcaacaccaa ggccgtttgt cgcaagtgct acattcatcc
      961 cgctgtgatc gacacgtaca tggaaggaga gctggccaga tcgctgaagc ggcgggtgga
     1021 gcagaagctg aagcgatcgc tgggcgccct ggcgccggaa gaagccgcgg tgctggcgct
     1081 gctgcagcag cggctggcga aggcgagcaa gttaattggc aaaaaagcgg cgtgaagcaa
     1141 atccgctgcg cggatgatat gctccagcgc ctcatcgccg cttgcggttt agcatctgtt
     1201 caacccctga tcgaaaagac gctatgccgc aagcggcgga acggattgat tacattggag
     1261 ttcactgtat ggcgtggctt tttctgctgc tcgcgggcgc gtgtgagatg atctggccac
     1321 tcgggttcaa atacacccac ggattcaaaa cgcattaccc gttcatcgcg ctgacgatgg
     1381 cgatgatgac actgagcttt tggctgatgg cgcaggcaac ggcgaagggc attcccgttg
     1441 gcaccgcgta cgcggtgtgg accggcatgg gcgcgaccgg gacggcggtg ctggggatgg
     1501 tctggttcaa ggagccgcgg gatgcggcgc gcatcgcgtg tctgctgatg atcgtcgtgg
     1561 gggtggttgg gttgaagtgg ttgtcgcctg ttgctgctgt tgctacgacg gcagctagtc
     1621 gctgaagcgg ttagtccgat tggaggtgtg ggtatgggga agcgttctgc gaaggcggta
     1681 gcgctgatcg atgtggagcg cgagatcgtg gtgacgcgaa aggtgctcgc gttggcgtgc
     1741 gttgcgttcg gcgcgttggc gttcgccgcc gcgccggcga gtcagccgca gacggtgcac
     1801 ctggtagttc acgtcgcgga cgttcgaaat caaaacggtc agctcatctt cggcgttttc
     1861 gcgcagccgg atgctttccc gaatatccag agcaaatctg tgaattggca agtgaagccg
     1921 gccagtgggg atggcgtgtt cacctgcgat ctgtcgccgg gggtttatgc ggcgtcggtg
     1981 ctgcacgatg agaacggcaa cggcgacatg gaccgcaatt ttgccggggt gccgacagaa
     2041 gggtacggcg tgacgaacaa ccccaagcca aaatatcggc aggcgacatt caaagaagcc
     2101 acgttcacac tacctgccgg cggagcggag ctgacaatca gcatccaata caagtactac
     2161 tgacgcctcc actggcgcgg ctgccggact tcgccgcaat ctcagaacgc gcgcggcgcg
     2221 ccgccgttac ctataagcga atatcgggcg actcccgccg gtgaaccagt gggcgccgtc
     2281 gtcgatgacg ctcccgtcaa accgcgccag ctcgccgacc ttctcggccg gcaccttgaa
     2341 ctccacgacg tgctccaagc agtccatatg cggcttgggg aactcgtgct ggaatgcctg
     2401 gttgatcaga atgatttctt tgaacggccc cttatcggtt cagatgagaa gcgtcggcgt
     2461 gacaccgttg ggctggccgt acttggccac cgtttgcttg aatgccttct gacaatcttc
     2521 gggccactgg gcgaccatct gctcggcaga catctgctgc cgatcagcgg cggcgcgttg
     2581 atgcgacggc tgcggcgctc gctcttgtgc gaaggtgaat ccggtggcgg gaagcaacaa
     2641 atacgatcgc cgcatacgcg gccgcgcgaa tgtgtgtcat cgtgtgattc ctttgcgagg
     2701 tagaaattca aaacgatccc gccttcctcc tcccgttcag tcgcgccgct tgtgatggtg
     2761 caaccggtgg tcatcatcgt cttcatctcc atccctgatc ggacgtgggc tgaacgccgg
     2821 ttgctgttgg gcgtggctca gcgagatggc agcgattccc tggggatgcg cagtcgcggg
     2881 cacctgcgac accgggaaga tcagtggaga cgttttccgt tccgacagcg cgccgtcctc
     2941 gtccacccgc aggacatgca gctgattccc gccggcgaag cccggcggcg gggcggtttc
     3001 gtggttcacg acataaagca atcgccccgt cgggtccagc gtcatctgga agtcgaccgt
     3061 ctgattagcc gtcgcgccag gcagatgtcg cggacccgcc agcgaaaact cctgaatctg
     3121 aaccggatga agcggatccg aaagtgagta gacgccgacg tcgtcgctgg cggagttagc
     3181 gacgtacagg tacttcccat cggcactcac caccaaccag caggcggcca tgccttggtc
     3241 ggcgatggga ccgcggaacg tgacggtgcc cgcgctgtcg taagtgaaaa cgccgacttc
     3301 gttcgcgccg gtcaatcccg cgtaaaggat gttggcggtc gggtgctggg ccaaacccag
     3361 tactaatggg ggcgtgaccg aggcgccgac cgctcccaag gctttaagcg aaccgttgct
     3421 gcccacccgc agcgtctcaa tcgtgttcgc atcggtcacg ttcagcagcg gcggtggggt
     3481 gaaattatcg ccgaagacga acctgccggt tcgggagatc aatgactggg ccggcgataa
     3541 tccgacaggc agtgtaattg tagaattagc aattggagtc agcgagccat cgctgtgcat
     3601 gaggaagccg gtgtagtttc cgctcaccgt gccggtctgg ccctgaatct cgtcaccacg
     3661 gttggtgaca taggttttgt cgccgtccac acccagactc acgggttgaa cgccgttgga
     3721 ggaaaacggc gcccccggta cgaggctcag gctgccatcg ctgcggattt taaagacggt
     3781 aatgctgttg ctgccctggt tgacggcgag caggaagcgg tgatcagcgc tgagctccac
     3841 ctcctggtcg gaatcatccg gacccaacgc ggcagtggga ttggcgatgc ccgttccgcc
     3901 ggtcaggaac ggactactgg acaattcgct cagcgtcccg ttcgcgttgc gcgtgaaggc
     3961 taacaccgcg ttttgaccag cggcgggatt gttgctttcg gtgtagacga cggtggacaa
     4021 catcgtgcgc gattcaagtg gttcgaaaaa cgtattctgc gaattcggcg acttctgcga
     4081 atgagggtta aagctgaagc agttgaacat cgcggctcct gaaaaaagga ctagcgcgcc
     4141 acccacgtgt cttgtgcacg aacccgcttc tgcgaatctg tcccgacagt cttcgcgaag
     4201 cacccattag tcgcagtctg ctgaaaaccc ttgcatgtct ttttcccaat cttttttaaa
     4261 ttgcgatcgg tccgcgcccc ccgcgcgtgc ggagaccggg cggcgccgcc gccgcgtctc
     4321 gtggagaggt gaaactgatg tcgcaagagg aagtgatgat cgaccaggca tcgtccgatc
     4381 gcgctgttca atggctggac gatcacggcg atgcgctcta cgcctatgcg gtgctgcgcg
     4441 tgaggcagag tcaggacgct gaagatctcg tccaggaaac attgctcgcc gccatcagag
     4501 ctcgggatgc attcgacgga gatgcgtcgg agcgcacctg gttgatcagt atcctaaagc
     4561 acaagatcgc ggaccacctt cgtcgcgcgc ttcgtgatag cggcaccggt cacaccgacg
     4621 gcgatgccgc cgttaaggcg gtggaaccct tcgataaacg tggggtttgg acggtatcgg
     4681 tgaagcgctg gccgggaacc ccgtccgagc ttttggagca agccgagttt cgcgatgtgc
     4741 tggctcgatg cctggccaag ctgccggcgc gaatggcgca gttgttttgg ttgcgcgcgg
     4801 ccgagggtaa gcgcaccgca gcggtgtgtg aggagctgga gatctccacc gataatgcct
     4861 ggacgctgct gcatcgcgcg cgatcgcgac tgcgccagtg cctggcaagc aattggtttg
     4921 ggcgcgggga tggtcgatga tgcggttgct gcgcgatctg cggcttttga acctcaactg
     4981 ccgcgaagca acgcgactcg tctcagatgc gctggaccgc aacctgcgcg ttttcgagca
     5041 agcgggcctg tccgcacatc tcgcgctctg caagaattgc aggcatttcc ggtcgaatct
     5101 aaagttctta gaagaattaa tcgacgcagc ggatctcgtc tctttttccg tctcgaccga
     5161 cgcgcttcca ccggaagcca aatcgcgcat cggtcgcggc atcgatcaat tgacgcgaac
     5221 aagcggggac gccagcggag ggggaggggg acagataacc gggagcgatt aaggagacat
     5281 agcggctctg tctcaaccct ccaagtggct gggccagatg cacaccctcc gcccgcccgg
     5341 ccgcccgctg aagcgcgcga ccgaacgatc aaaagcggcc caacccgatg caatagtgga
     5401 aacggattcc gtccccgttt catttccccg gattccgtcc ccgtttcatt tccccccgtt
     5461 tcattcacgt ccccgtttca ttcaattgcc cccgttttat tcaaccattc ctttggcgct
     5521 tccggagggt gcttcggagg cttgtttcat tccggcgatc aagtgccaat ggtttcaggc
     5581 atgacaatca cgggcgatgg tttgccgggc cgatccgtgc gacaaacccg ccgccggggc
     5641 gcagtttgag ggtgagcgtg tcgccggggc cgaggggctt ttcctggcgg tcgagggcgt
     5701 catctttgcc gggcacatcg cacaaaagca ccgagctttg cgccgttttc aggaagtcga
     5761 gcgagattgg caactcgcgt tcggtcgccc cgttgaccac gccgatccac cacgtgtcgc
     5821 ccttcctgcg ggcgaagccg gcgacttcgc cgatcaccgt gccgggc
//
LOCUS       DATIMH010000880         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4252167, whole genome shotgun sequence.
ACCESSION   DATIMH010000880 DATIMH010000000
VERSION     DATIMH010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252167"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VLI90_15265"
     CDS             <1..801
                     /locus_tag="VLI90_15265"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HSV15619.1"
                     /translation="RSFGVEVQHELPGGGENYRDHYAPRMNWRVKLPITLNEQTRGIG
                     FAREIARYFMTGRGILTYTAGIAYGFVKTRPELEEPDVQFHFAHASYGNAANRVLERE
                     PGMTVTTYQCRPESQGSIHLRSPDPLAAPCIRPNYLADPLDQRVVVDGMKIARRIVSN
                     AAMDKYRAFEMNPGDAVQTDEQWLDFARHNGQTTYHVVGTCKMGRDPMAVVDDELRVH
                     GIQGLRVVDASVMPTVVSGNTNAATIMIAEKGADMIRAAARGGMAQAA"
     gene            822..1379
                     /locus_tag="VLI90_15270"
     CDS             822..1379
                     /locus_tag="VLI90_15270"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV15620.1"
                     /translation="MTLAEFLAWEERQGLRYEFDGTQPLARVGGTVAHSAIQCNLAIA
                     VGGRLRGRQCQFFGSSLKIEVAGRICYPDAFVVCSPVPMRSTLVRDPVVIFEVLSDST
                     AQTDIVTKNHEYAATPSVQRYVILAQDEIGGTMFERIGGDWVGHVLGADAVLRMPEIG
                     VEVSISEFYEGVDLSTAGTADETER"
     gene            complement(1562..2386)
                     /locus_tag="VLI90_15275"
     CDS             complement(1562..2386)
                     /locus_tag="VLI90_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV15621.1"
                     /translation="MSSYVSSTALANYGVLRAAASGGASGNYSAFPPGYAQDYAHEVL
                     VATYNGDATALYNDAITLISGNKPYGTPVVVRFGFSLHDLIAYTGGIAGSNLAALSYY
                     AGGETGSSQDTATGANFHTFLLDVNAYVGETLTFGAALAAAAAGNTVLPGGIVQVQSS
                     VNALSTSYSSIFVLDPAIGYIAESGTTYLTSLPDEVAAVPEPPALSMLGIAVIGIAGV
                     KRRRRSVAADMADRGRPCPPEEGLVDVGRVDRFPRRSGRGLSGRKLPYRLPVRDSL"
BASE COUNT          460 a          770 c          804 g          404 t
ORIGIN      
        1 cgctcgttcg gcgtggaggt gcagcacgag ctgcccggcg gcggcgagaa ctaccgcgac
       61 cactacgcgc cgcgcatgaa ctggcgggtg aaactgccga tcaccttgaa cgagcagacg
      121 cgcgggatcg gcttcgcgcg cgagatcgcg cgctacttca tgaccggccg cggcatactc
      181 acctacaccg ccggcatcgc ctacggattc gtcaagacgc ggcccgagtt ggaagagccc
      241 gacgtgcagt tccacttcgc gcacgcgagc tacggcaacg ccgccaaccg cgtgctggag
      301 cgcgagcccg gcatgacggt gacgacctac cagtgccgcc cggagtcgca gggctcgatc
      361 catctccgct cgcccgaccc gctggcggca ccgtgcatcc ggccgaacta tctcgccgac
      421 ccgctcgacc agcgcgtcgt ggtggacggc atgaagatcg cgcggcgcat cgtgagcaat
      481 gcggcgatgg acaagtaccg cgccttcgag atgaaccccg gcgacgcggt gcagaccgac
      541 gagcaatggc tcgacttcgc ccgccacaac ggccagacca cctatcacgt cgtcggcacc
      601 tgcaagatgg gccgcgatcc gatggcggtg gtggatgacg agttgcgggt gcacggcatc
      661 cagggcctgc gcgtggtcga cgcctcggtg atgccgacgg tggtctccgg caacaccaac
      721 gcggcgacca tcatgatcgc ggagaagggg gccgacatga tccgcgcggc ggcgcgcggg
      781 gggatggcac aggcggcttg aaccttgccc tcggcgaacc cgtgacactg gccgaatttc
      841 tcgcgtggga agaacgccaa gggttacgct acgagttcga cggaacccag ccgctcgcca
      901 gagttggcgg aacagtcgcg cactccgcga tccagtgcaa tttggcgatc gctgtcggcg
      961 gtcggctccg cggccggcag tgccaattct ttgggagcag cctcaagatc gaggtcgccg
     1021 gacgaatctg ctatccagac gccttcgtag tctgctcacc agtcccgatg cggagcacgc
     1081 ttgtccgcga tccggtggtg atcttcgagg tgctgagcga cagcacggca cagacggata
     1141 tcgtcaccaa gaaccacgaa tacgccgcca cgccctcggt ccagcgctat gtcatcctgg
     1201 cccaggacga gatcggcggg acgatgttcg agcgcatcgg cggcgattgg gtcggtcacg
     1261 tccttggggc ggatgcggtt ttgcggatgc cggagatcgg cgtcgaggtg tcgatttcgg
     1321 aattctacga gggagtggat ttgtcgaccg cggggacagc ggacgaaacc gaacgctgag
     1381 cggcatcagc ctgggactgg agcgtgatct cttgaagtcg gctcatccgc gtcatcgcga
     1441 gccgcgtagc ggcgtggcga tccatcgtgt ttacgggcgg gtagccacct ggattgccac
     1501 gccgctacgc ggctcgcaat gacgccgttg cgtgtgtgat tcgacgcgaa gtgatgatgc
     1561 tttataggct gtccctgacc ggcaggcggt agggcaattt ccggccactc agccctcggc
     1621 cagacctccg cgggaaacga tcgacgcgac ccacatcgac gagtccctcc tcgggagggc
     1681 acggccgacc gcgatccgcc atatcggcag ccaccgagcg acgccgccgt ttcacgccgg
     1741 cgatgccgat tacagcgatc ccgagcatcg acagggccgg aggctcgggg accgctgcta
     1801 cctcgtcggg aaggcttgtg agataggtgg tgccactttc ggcgatataa ccaatggcag
     1861 gatccaatac gaaaatgctg ctgtatgagg tgctcagggc atttacactc gattggactt
     1921 gaacgatccc gcctgggagc acggtattgc cggcagccgc agcggccagc gcggcgccga
     1981 acgtaagcgt ctcgccgaca tatgcattca catcgagaag gaacgtatgg aaattggcgc
     2041 cggtcgccgt gtcctggctc gaaccggtct cgcctccggc ataatagctc aacgcggcca
     2101 agttcgagcc agcaattccg ccggtatacg caatcaaatc atgcagggaa aacccgaacc
     2161 tcaccaccac cggggtgccg tacggcttgt tcccggagat gagcgtgatc gcgtcattat
     2221 acagcgcggt ggcgtcgccg ttgtaggtcg cgacaaggac ttcgtgcgcg taatcctgag
     2281 cgtaccccgg cgggaaggcc gagtagttgc cggaggcacc accggacgcg gccgcccgca
     2341 gcacgccgta attcgccaac gctgtgctgc tgacgtagct gctcaacccg gggcgagaac
     2401 ggattggccg catagggtgt tacggattgc ctgatagg
//
LOCUS       DATIMH010000881         4262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_790445, whole genome shotgun sequence.
ACCESSION   DATIMH010000881 DATIMH010000000
VERSION     DATIMH010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790445"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VLI90_15280"
     CDS             complement(<1..435)
                     /locus_tag="VLI90_15280"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HSV15622.1"
                     /translation="MGAPSSEAIATPPHAQKELLRPVESLAGVGPTRAKELRRLGIAT
                     LGDLLEYFPRDYQYESAEKPISQLVAGQIEMARGEVVAVDYIGARPRARFEATLDDGT
                     GKLGLIWFNAPYLRKSIHPGKYIRVQGAVKTYRNLPQMTHPTW"
     gene            complement(627..1424)
                     /locus_tag="VLI90_15285"
     CDS             complement(627..1424)
                     /locus_tag="VLI90_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15623.1"
                     /translation="MEAVPTHTSPAVFQCRSPNPPSAAWRVAMMMLVGAMVGAVAWYG
                     MDQNLTLAHVAGWLLAGIAVFTPFAWFIERNPVFSIEIADTGLTIVRANGTRQLAWAD
                     IEAARLQGYPFAHSGGQTIRCFLLRAAGETFELTPEFIDNATRDAFEGALFRELESRD
                     IPETAKALPSFDRMLSLGGAWLFIASIAGILAAQAAGFHTLGTILGSASLLTGSSIAW
                     MTRQQRLSRVVLISTIFLIVASSAILWSCHVNVRDELQKLEWMEKRQ"
     gene            complement(1536..2192)
                     /locus_tag="VLI90_15290"
     CDS             complement(1536..2192)
                     /locus_tag="VLI90_15290"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HSV15624.1"
                     /translation="MNDVKDETRQETMIQQQVVERGIRDERVLAALRTVPRDKFFPAG
                     SKDEAFADRAAPIGHGQTISQPYMVALMTQRLDVQPDHKVLEIGTGSGYQTAILAQLA
                     RDVYTIERVKPLLDDAWERLMDMGYRNVHFRHGDGTTGWSEAGPFDRILITAGAPVLP
                     EKLLMDQLTDGGIAVVPVGPHDEQMLIEVRREKSKLLTSDVCPCRFVKLIGQEGWAKE
                     "
     gene            2312..3247
                     /locus_tag="VLI90_15295"
     CDS             2312..3247
                     /locus_tag="VLI90_15295"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HSV15625.1"
                     /translation="MFSQIILGVDLGVLGDSAVNQALEIESKEPKDGKAGDAIAGFTS
                     NYRFLYLYRMPRKARRHRRPIITLTSDFGLTDSYVAAMKAVLLRHCPEATLIDVTHNV
                     PRQDILCGSITLERAVDGFPPGTVHLAVVDPGVGSDRRILVAELNESFIVAPDNGLIT
                     WSWRVHPGGRAHELTWRPRKHSDTFHGRDIMAPAAGMIAAAEDLSKIMRPLGDPILLD
                     VSPADDARGQIIQIDHFGNATTNIPYERIRALATKSVRVRNRDLGRLRRIYQDAAPGK
                     PLALIGSSGLLEIAVRDGSASEDLKIRVGDEVQLR"
     gene            3295..>4262
                     /gene="bioA"
                     /locus_tag="VLI90_15300"
     CDS             3295..>4262
                     /gene="bioA"
                     /locus_tag="VLI90_15300"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HSV15626.1"
                     /translation="MSYTTDHLRAKDKQHLWHPFTPMSLWDKSDPLVITAAQGMHLID
                     SDGNRYLDGVSSLWCNVHGHRVPEIDAAVRAQLDKVAHTTMLGLSNEPAILLAERLMR
                     IVPQNLKKVFYSDAGATATEIALKLAAQYWFNIGRGEKCEFVGFAEAYHGDTFGAMSV
                     GRTATFHKPYFPLLFKTHFAPSPYVYRSDTPHDPAAVGDRCLSELETILQQHGSRIAA
                     VCIEPIVQGAGGMIVQPDGFLRGVRELCSKYGVLMIADEVAVGFGRTGRMFACEHESV
                     EPDLMCVAKGISGGYLPLAATFATQQIFDAFLGEPWEGRTFYHGHT"
BASE COUNT          781 a         1339 c         1351 g          791 t
ORIGIN      
        1 ccacgtcgga tgggtcatct gcggcaggtt tcgatacgtc ttcaccgccc cctgcacgcg
       61 gatatactta ccggggtgga tgctcttgcg caggtacggc gcgttgaacc agatcagccc
      121 gagcttgccc gtgccgtcat cgagcgtcgc ctcgaaacgc gcccgaggcc gcgcgccgat
      181 gtaatccacg gcgaccactt cgccgcgcgc catctcaatt tgccccgcga ccagctgcga
      241 aatcggcttc tccgccgatt cgtactgata atcccgcgga aaatattcca gtagatcacc
      301 cagcgtcgcg atgcccagcc ggcgaagttc cttcgcgcgc gtcggcccaa ccccggccag
      361 cgattcgacc gggcgcagca attctttctg cgcgtgcggg ggcgttgcga tggcttcgga
      421 cgaaggtgct cccattcgac agaagtgtag cgttgtcgcc ggcgtttctg tagccgcgac
      481 tggcatgtcg ctcacaacgc cggcgcaggt gtcgccggtg gtggtcagcg aattcgtctt
      541 gcaggttctc tctcagcccc tcgatccggt tggccgcttc accgttgtcc gtgaaggctc
      601 ggtgcggatg atcccgttac cgcgagctac tgtcgtttct ccatccactc caacttctga
      661 agctcgtcgc gcacgttcac gtggcacgac cataggatcg cgctgctcgc cacaattagg
      721 aaaattgtgg agatcaacac gacccggctg aggcgttgtt ggcgggtcat ccacgcgatg
      781 ctgctgccgg tgaggaggga cgcggagcct aggatggtgc cgagcgtgtg aaagccggcg
      841 gcctgggcgg cgaggatgcc ggcgatgctg gcgatgaaca gccacgcgcc cccgaggctg
      901 agcatgcggt cgaagctggg gagggctttg gcggtttcgg ggatgtcgcg cgattcgagc
      961 tcgcggaaga gcgctccttc gaatgcgtcg cgcgtcgcgt tgtcgatgaa ttcgggggtg
     1021 agttcgaatg tctcgccggc ggcgcggagc aggaagcagc ggatcgtctg cccgccgctg
     1081 tgggcgaacg ggtaaccctg caaccgggcg gcttcgatat cggcccaggc gagttgtcgc
     1141 gtgccgttgg cgcggacgat cgtcaagccg gtgtcggcaa tttcgatcga gaagacggga
     1201 ttccgctcaa tgaaccacgc gaacggcgtg aacaccgcaa tgcccgcgag aagccagccg
     1261 gcgacgtggg caagggtgag gttctggtcc atcccgtacc aggcgacagc gccgaccatc
     1321 gcgccgacca gcatcatcat cgccacgcgc caagcggcgg atggtgggtt gggcgaccgg
     1381 cactgaaaga cggccggtga cgtgtgggta gggacggctt ccatcgccgg agaagtttac
     1441 cacgacggca agatggacag gctcaaaagg tcgatttgca gctatctttt cgcgcgagtt
     1501 cggcacaccg ataaggagcc aagagcgtaa gcgggctatt ccttcgccca gccctcttgc
     1561 ccgatcagtt tgacaaaccg gcaggggcag acatcgctgg tgagcagttt tgacttctcc
     1621 cgccgaactt cgattagcat ttgttcatcg tgcggaccga ccggcacgac cgcgatgccg
     1681 ccatcggtca gttgatccat caacagcttt tccggcagca ccggcgcacc ggcggtgatg
     1741 aggatgcgat cgaacgggcc ggcctcggac caaccggtgg tgccgtcgcc atggcggaag
     1801 tggacgttgc gatagcccat gtccatcagg cgttcccacg cgtcgtcgag cagcggcttg
     1861 acgcgctcga tcgtgtacac gtcgcgggcg agttgcgcga ggatcgcggt ttgatagcca
     1921 ctgccggtgc cgatctccag gactttgtga tccggctgca cgtcgagccg ctgggtcatc
     1981 agcgcgacca tgtacggctg gctgatggtc tgtccgtgac cgatcggcgc ggcgcgatcc
     2041 gcgaacgctt catccttgct gccggctggg aaaaatttat cccgcggcac ggtgcgaagg
     2101 gcggccagca cgcgttcgtc gcgtatgccg cgctccacca cctgctgctg gatcatcgtt
     2161 tcctgccggg tttcgtcttt cacatcattc atgctgttgg ccattgtacc gctggaaagc
     2221 cgacgatggt atgtcgccgc gcgggtgcta ccgtccttcc aattctctgc gctcaattca
     2281 ccgccgagac gccgaggcag acgccgagat tatgttctca caaattattc tcggcgttga
     2341 cctcggcgtc ctcggcgact cggcggtgaa ccaggcgctg gaaatcgaat cgaaagaacc
     2401 caaggatgga aaagccggcg atgccatcgc cggctttaca tcgaactatc gattccttta
     2461 cctttatcgc atgcctcgca aggcgcgccg ccaccgccgg ccgatcatca cgttgacgag
     2521 cgacttcggc ctgaccgatt catacgttgc cgcgatgaaa gcggttctgc tgcggcattg
     2581 cccagaggcg acgctgatcg acgtcacgca caacgtgccg cgacaggaca ttctatgcgg
     2641 ctcgattacg ctagagcgcg ccgtcgatgg ctttccgccg ggcacggttc accttgccgt
     2701 ggtggacccc ggcgtcggat cagaccgtcg aattcttgtt gccgagctga atgaatcgtt
     2761 catcgtcgcg cccgacaacg ggctgatcac gtggagctgg cgcgtccatc ccggcggccg
     2821 ggcgcacgag ctgacctggc ggccgaggaa gcacagcgac acgttccacg gccgcgacat
     2881 catggccccc gccgccggca tgatcgcggc ggcggaagat ctctccaaaa tcatgcgacc
     2941 gctcggcgac ccgatcctgc tcgatgtcag ccccgcggac gacgcccgcg ggcaaattat
     3001 tcagatcgac cacttcggca acgcgacgac gaacattccg tatgaacgca ttcgcgcgct
     3061 ggcgacgaag tcggtgcgag tgcgcaatag agacctcggc cgcctgcggc ggatctacca
     3121 ggatgcggct ccgggaaaac cgctggcgct gatcggcagc agcggactgt tagagatcgc
     3181 cgtgcgcgat ggatccgcat cggaagatct gaagatccga gtgggggacg aagttcaact
     3241 ccgatagaag ttacccacaa aagcccacgg ctctgccgtg ggacctccac aaagatgtcc
     3301 tacaccaccg accatcttcg cgccaaagac aagcagcacc tctggcaccc gttcacgccg
     3361 atgtcgctgt gggataagtc ggacccgctc gtcatcaccg ccgcccaggg gatgcacctg
     3421 atcgattccg acggcaaccg ctatctcgac ggcgtctcca gcctctggtg caacgtgcac
     3481 ggccaccgcg tgccggagat cgacgcggcg gtgcgcgcgc agctcgacaa ggtcgcgcac
     3541 acaacaatgc tcggcttatc aaacgaaccc gcaatcctgc tggccgagcg cttgatgcga
     3601 atcgtcccgc agaatctgaa gaaggtgttt tatagcgacg ccggcgcgac ggcgacggag
     3661 atcgcgctca aactggcagc gcaatactgg ttcaacatcg gccgcgggga gaaatgcgag
     3721 ttcgtcggct tcgccgaggc gtatcacggc gacacgttcg gggcaatgtc ggtcggccgg
     3781 acggcgacgt ttcacaagcc gtacttcccg ctgctgttca agacccactt cgcgccgtcg
     3841 ccgtacgtgt atcgcagcga cacgccgcac gatcccgcag cggtgggcga tcgatgcctg
     3901 agcgagctgg agacgatcct gcagcagcac ggcagtcgca tcgccgccgt ctgcatcgag
     3961 ccgatcgtgc aaggcgccgg cgggatgatc gtgcagcccg atggttttct ccgcggcgtg
     4021 cgcgagctgt gcagcaagta tggcgttttg atgattgcgg acgaagtggc cgtcggtttc
     4081 ggccgcaccg gacggatgtt cgcctgcgag cacgaatcgg tcgagccgga tctgatgtgc
     4141 gtcgccaagg gcatcagcgg cggatatctg ccgctcgcgg cgacgttcgc aactcagcag
     4201 atcttcgacg cgtttctcgg cgagccgtgg gaaggccgga cattctatca cgggcacacg
     4261 ta
//
LOCUS       DATIMH010000882         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2809448, whole genome shotgun sequence.
ACCESSION   DATIMH010000882 DATIMH010000000
VERSION     DATIMH010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2809448"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VLI90_15305"
     CDS             <1..577
                     /locus_tag="VLI90_15305"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HSV15627.1"
                     /translation="YVPRDHQVRVIAGSAGCDLAEVSSPVTGTYPLQFVPFEAVRRDP
                     NLHFAAGAPSCERDLNIVIGKNVQAGRIMAGVTFSKPGNWTSWPPHEHGRLLEEAYLY
                     IDMPAPAWGLQLVFTDNEQPELIAKVTEGDVVVMPAGYHPNVAAPGSCINFMWMMAAH
                     TEVTDRQFGVVNVHPDYAAGGTGLEASRETK"
     gene            583..>2239
                     /locus_tag="VLI90_15310"
     CDS             583..>2239
                     /locus_tag="VLI90_15310"
                     /inference="COORDINATES: protein motif:HMM:NF027479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HSV15628.1"
                     /translation="MRFFTAILLAAAVHAAPHIHRLQLSVSNPTAVARSAENIVVSVP
                     ALKKLAPDFNAANAIVVATSAATFDADALAMQANELPSQADDLDGDGKLDELVFQIAL
                     APNQTRIVTIAWGEQAAIQAIRGAYPARTAMKFATRYEGLGWESEDIAWRIYFDKRNA
                     IDIYGKRRPGMYLDLFASPEYVYHLESPFGRDIFMVAPTLGVGSVGALIDGKAMPVAD
                     VGDRKWRVVAAGPVRSIGEYEFKAWRIGGKTIDMVSRFTQWAGEHGFHHRVTVSDPAA
                     IQLTAALPKKPGIAPLPTPLGSNVAGVVTWGHQVVVPGAKAGTTELPDENLGLALIGR
                     NESLASKSEDAANYLFGLARGSNTSEWYTAALWDQENTAALTVSAAAPAARNQGGSLV
                     LASPPSTRERFQAIVQEAAAQVGAPAVVKFGAVLPEAAPVATAGRTYKEAIALLQKAA
                     ARTASKFEPLVSSTAPTAYDKFTGPGFFTEGNGQTGEWTPQQGYFWTGGFWAGELWKL
                     SSVSDEPRFKKWAELWTSRLLGNEHKQNHDTGFLNFYSSVAAYE"
BASE COUNT          412 a          725 c          714 g          388 t
ORIGIN      
        1 ctacgtgccc cgcgatcatc aggtgcgcgt catcgccggc agcgcaggct gcgatctcgc
       61 cgaggtctcg tcgcctgtca ccggcactta tccgctgcaa ttcgttccct tcgaggcggt
      121 acgccgcgac ccgaacctgc acttcgccgc tggcgcgccg agttgcgaac gcgacctcaa
      181 catcgtgatc ggcaagaacg tgcaggccgg gcgcatcatg gcgggcgtca ctttttccaa
      241 acccggcaat tggacctcgt ggccgcccca cgaacacggc cgcctgctgg aagaggccta
      301 cctttatatc gatatgcccg cgccggcctg gggtctgcag cttgtgttca ccgataacga
      361 gcagcccgaa ctgatcgcca aagtcaccga aggcgacgtg gtcgtgatgc ccgcgggcta
      421 ccatcccaac gtggccgcgc cgggcagttg catcaatttc atgtggatga tggccgcgca
      481 taccgaggtc acagaccgcc agttcggcgt cgttaacgtg catccggact acgccgcggg
      541 cggcaccggt ctcgaagcct cgcgcgaaac gaaatgacgc ccttgcgctt tttcaccgcg
      601 attctgctgg ccgcggccgt acacgccgcg ccgcacattc accggcttca gctctcggtg
      661 tcgaatccga cggccgtggc gcgcagcgcg gagaatatcg tggtttcggt gccggcgctc
      721 aagaagctcg cgcccgattt caacgccgcc aacgccatcg tcgtggcgac cagcgcggcg
      781 actttcgacg cggacgcgct cgccatgcaa gccaacgaat tgccctcgca ggccgacgac
      841 ctcgatggcg atggcaaact cgacgaactc gtctttcaaa tcgcgcttgc ccccaatcag
      901 acgcgtatcg tgaccattgc gtggggcgag caggcggcga ttcaggctat acggggcgct
      961 tacccggcgc gcacggccat gaagttcgcc acccgctatg agggcctcgg gtgggaatcg
     1021 gaagacatcg cgtggcgcat ctacttcgac aagcgcaacg ccatcgatat ttacggcaag
     1081 cggcgtcccg ggatgtacct cgatctgttc gcctcgcccg agtacgtgta tcacctggaa
     1141 agcccgtttg gccgcgacat ctttatggtg gcgccgacgc tcggcgtggg ctcggtgggc
     1201 gcgctgatcg atggcaaagc gatgccggtg gccgacgtcg gcgaccgcaa gtggcgcgtg
     1261 gtggccgcgg ggccggtgcg ctccatcggc gagtacgagt tcaaagcgtg gagaatcggc
     1321 ggcaagacca tcgacatggt cagccgcttc acccagtggg caggcgagca cgggttccat
     1381 caccgggtca cggtcagcga cccggcggcg attcaattga cggcggcgct ccccaaaaag
     1441 cccggcatcg cgccactgcc aaccccgctt ggcagcaacg tcgcaggcgt ggtgacctgg
     1501 ggccaccagg tggtagttcc cggcgccaaa gcgggcacta cggaactgcc cgacgagaat
     1561 ctgggactgg cgctcatcgg gcgcaacgaa tcgctggcgt cgaaatctga ggacgccgcc
     1621 aattacctct tcggtcttgc gcgcggaagt aatacgtcgg agtggtatac ggcggcgctg
     1681 tgggatcagg agaatacggc ggcgctcacg gtgtctgccg cggcacctgc ggcacgcaat
     1741 cagggcggca gcctggtgct cgcttccccg ccctcgactc gcgagcgctt ccaagcgatt
     1801 gtgcaggaag cggcggcgca agtcggcgcg ccggctgtgg tgaaattcgg cgcggtgctg
     1861 ccggaagccg cccccgtcgc gactgccggg cgtacttata aggaagcgat cgcgctgctg
     1921 caaaaggcag cggcacgaac ggcgtcgaag ttcgaaccgc tggtctcgtc aacggctccc
     1981 actgcgtacg ataagttcac gggtcccggc ttcttcacgg aaggcaacgg gcagaccggc
     2041 gaatggactc cccagcaagg ctatttctgg accggcggat tctgggccgg cgaactctgg
     2101 aaactctctt cggtcagcga cgagccccgc ttcaaaaagt gggccgaatt gtggacgagc
     2161 cgcctgttgg gcaacgagca taaacagaat cacgataccg ggtttctaaa tttttactcc
     2221 tcggtcgcgg cctacgaaa
//
LOCUS       DATIMH010000883         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3677744, whole genome shotgun sequence.
ACCESSION   DATIMH010000883 DATIMH010000000
VERSION     DATIMH010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3677744"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VLI90_15315"
     CDS             complement(<1..342)
                     /locus_tag="VLI90_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15629.1"
                     /translation="MHATIHLDTQHHVGPIDRRIFGGFLEHLGRAIYGGVYDPGNALS
                     DERGFRRDVLDVLRPMRMPVVRYPGGNFVSCYDWRDGVGPRDRRPVRTDYAWRSLEPN
                     QFGVDEFLAWSG"
     gene            475..1404
                     /locus_tag="VLI90_15320"
     CDS             475..1404
                     /locus_tag="VLI90_15320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV15630.1"
                     /translation="MATDSIPTVSVVTPSFNQGAYIQSTIRSVLSQDYPRIEYLVMDA
                     ASTDNTLDLLRKYSGERPDRMRFVSEKDRGQSHALNKAVAQTSGEIIGWLNSDDTYEP
                     GAIAAAVECFRQHPDVDLIYGDANFTDPQDRVLARCTHIEPYNWHRFVHYSDFIVQPA
                     AFFTRRAFEAVGGADESLHYVMDYDLFLKMASKFKVAYLPRVLANYKWFGQNKTAVGG
                     WDRLNEMRRVAMAFGARGLPAYGRLEAARLGVSLAIGELRHAHITRAARQFATGAAPF
                     LTSPRAWRSLVSPACWRIIWTGQVLRRANRLTK"
     gene            complement(1414..2169)
                     /locus_tag="VLI90_15325"
     CDS             complement(1414..2169)
                     /locus_tag="VLI90_15325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV15631.1"
                     /translation="MDDAAFSALGIKHGLAYPFGLLTPGTIYSHACMAETRGPLIDAL
                     RKTELDDIDRWRLDLMERGLARLGDRWDFADNVTIAHAFAQLAAPKRVLEIGVRRGFC
                     TAAVAAGAPDAEIHLIDMWMANYSGVANPGPDLIRQQLDACGHRGPLHIHSGDSHAVL
                     PELFGAQPNLTFDLILVDGDHTAAGAALDLAQTAPRLAAGGLLVFDDLVHPGYPHLLS
                     LWRQFLEKIGSHARSFEYLDHGRGVGLALRVSH"
     gene            2284..>2727
                     /locus_tag="VLI90_15330"
     CDS             2284..>2727
                     /locus_tag="VLI90_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15632.1"
                     /translation="MNHPFLFSAAFTTIAMLAGAAVLHLLPRLGAVGRRTAEACCAAP
                     MLDVIITYFTIAPLFVAPAVTRSVWGFVGAVVGQLAGLILWTILHELAHLRSFNGPRI
                     LVNINRKVGIVRNMLATYWTLLVVPLFWFVRLAEIFAYPVLNWLVE"
BASE COUNT          464 a          821 c          936 g          506 t
ORIGIN      
        1 gccgctccag gcgaggaatt cgtcgacgcc gaattggttc ggttcgaggc tgcgccaggc
       61 gtaatcggtg cgcaccggcc ggcggtcgcg cggaccaacg ccgtcgcgcc agtcgtaaca
      121 gctgacgaag ttcccgccgg gatagcgcac gaccggcatg cgcattggcc gcaggacgtc
      181 caggacatcg cggcgaaagc cgcgttcgtc gctcagcgcg ttgccggggt cgtacacgcc
      241 gccgtaaatc gcccggccga ggtgctcgag aaacccgcca aagattcgcc ggtcgatcgg
      301 gccaacgtga tgctgcgtgt cgaggtggat ggtggcgtgc atagttattg tgattacgtg
      361 attacgtgaa aacggagtgg gcgtccactt atggcattca cgtattcgcg taattacgtt
      421 ccacgtcgcg ggacaagcgc tacgcgatcc gtataatccc cgcctcattc gttcatggcc
      481 actgactcaa tccccactgt ttccgttgtc acgccgtcct tcaaccaggg ggcgtatatc
      541 cagagcacga tccgcagcgt gctgtcacag gattatccgc ggattgaata cctggtgatg
      601 gacgcggcgt cgacggacaa tacgcttgat ctgctgcgga agtattcagg cgaacggccg
      661 gaccggatgc gattcgtctc ggagaaagat cgcgggcaat cgcacgcgct gaacaaggcg
      721 gtcgcgcaga cgagcggcga gatcatcggc tggctcaatt cggatgacac gtacgagccc
      781 ggcgcgatcg ccgcggctgt ggaatgcttt cggcagcatc ctgatgttga cctgatctat
      841 ggcgacgcga acttcaccga cccgcaggat cgcgtgctgg cgcggtgcac gcacatcgag
      901 ccgtacaact ggcatcgctt cgtgcattac agcgacttca tcgtgcagcc ggccgcgttc
      961 ttcacgcggc gcgcgttcga ggcggtcggc ggagccgatg aatcgctgca ctacgtgatg
     1021 gactacgacc tgtttttgaa gatggcgtcg aagttcaagg tggcgtatct gccgcgggtc
     1081 ttggcgaact acaagtggtt cggccagaac aagacggccg tcggcgggtg ggaccggctg
     1141 aacgaaatgc gccgcgtggc gatggcgttc ggggcccgcg ggctgccggc gtatgggcgg
     1201 ctggaagcgg cgcgattggg cgtgagcctg gcgatcggcg agctgcggca cgctcacatc
     1261 acccgcgcgg cgcggcaatt cgcaaccggc gctgcgccgt ttctcacctc gccgcgggcg
     1321 tggcgtagtc tggtctcgcc cgcgtgctgg cggatcatct ggacggggca ggtgcttcgc
     1381 cgcgcgaatc ggttgacgaa gtaatcggtg cgttcaatgt gagacgcgca gcgcgagccc
     1441 gacgccgcgg ccgtggtcga ggtattcgaa cgagcgcgca tgcgatccga ttttctccag
     1501 gaactgccgc cacagcgaga gcaggtgggg ataacccgga tgaaccagat cgtcgaacac
     1561 gagcaatcca ccggccgcga ggcgcggggc ggtttgcgcc aaatccagcg ccgcgccggc
     1621 ggcggtgtgg tcgccatcga cgaggatcaa gtcgaaggtc aggttcggct gcgcgccgaa
     1681 tagttccggt aaaaccgcgt ggctgtcgcc gctgtgaatg tgcagcggac cgcgatgccc
     1741 gcaggcatcg agttgctggc ggatcaagtc aggaccggga ttggcaacgc cggagtagtt
     1801 cgccatccac atgtcgatca ggtgaatctc cgcatccggc gcccccgcgg cgacggcggc
     1861 ggtgcagaaa ccgcggcgca cgccgatctc cagcacgcgc ttgggcgccg ccaattgcgc
     1921 gaacgcatgg gcgatcgtga cgttgtcggc gaaatcccat cgatcgccga ggcgcgccaa
     1981 gccgcgctcc atcaggtcga gccgccagcg gtcgatgtca tcgagttcag tcttgcggag
     2041 cgcgtcgatg agcggcccgc gcgtctcggc catgcaggcg tggctgtaga tcgttccggg
     2101 ggtgaggagg ccgaagggat aggcaaggcc atgctttatt ccaagggcag aaaaggccgc
     2161 atcgtccatg gggtgttctc ccgcgcgcgt gtgcgtgcgt gtgtcagatc gtctttcgcc
     2221 cggcatgata cggtggcgga ggcgcgaacg cacttgcggc aggccgcggt atgatacgcg
     2281 gccatgaacc atccctttct cttctcagct gcatttacca cgatcgcaat gctcgcgggg
     2341 gccgcggtgt tgcacttgct gccgcggctg ggtgccgtag gccggcggac ggcggaggct
     2401 tgttgtgcgg cgccgatgct cgacgtcatc atcacgtatt tcacgatcgc cccgctgttc
     2461 gtcgcgccgg cggtgacgcg cagcgtgtgg ggattcgtcg gcgccgttgt cgggcagctg
     2521 gcggggttga tcctctggac gatcctgcac gagctggcgc acctgcggtc gttcaacggg
     2581 ccgcgcatcc tcgtaaacat caaccgcaag gtcggcatcg tgcgcaacat gctggcgacg
     2641 tattggacgc tgctggtcgt gccgctgttc tggttcgtgc gcctcgcgga aatcttcgcg
     2701 tatcccgtgc tgaactggct ggtcgag
//
LOCUS       DATIMH010000884         5024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2521235, whole genome shotgun sequence.
ACCESSION   DATIMH010000884 DATIMH010000000
VERSION     DATIMH010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2521235"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1003)
                     /locus_tag="VLI90_15335"
     CDS             complement(89..1003)
                     /locus_tag="VLI90_15335"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HSV15633.1"
                     /translation="MAAQPLLAVVSVLGKDQKGVVAQFATYMADRGINIEDIEQRVVR
                     GFFVMDMLVDLKDLNIDLSELITGLLELGRKIDMEVKAHLHSEKRKKRVALLVSREPH
                     CLELLIDDFKTGRAHGEIAGVFANHPDLEPIAKQAGLPFAWHPAADNVQKHFDWLADQ
                     LKSAAADLVVLARYMRILPESLVQTYRYRIINIHPSLLPYFPGAAPYKQAYESGVRVH
                     GCTSHFVTEQLDEGPVIMQDVFHITVGADSLDDVKRKGLELEAHVLSKAVQLFLDEEL
                     VVVDGKVIFKPGLSRFLKSERSGGREAK"
     gene            complement(1048..2637)
                     /locus_tag="VLI90_15340"
     CDS             complement(1048..2637)
                     /locus_tag="VLI90_15340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV15634.1"
                     /translation="MNAPRQRLHLPVAILVLLGIVLGTYWPVLRNQFVDFDDQTEIYQ
                     NPDFNPVTARRVAWNWSHTRMTLYMPLTYTVWGGVAAISPREAHGTLTAWPFHALNLA
                     LHAACVLLVFLLLRQLGMTTISAAFGAAVFAVHPMQTEAIAWASGMYTLLGAALALGA
                     MIVYLRSGDGSRSRASRVALYALATAMYVAALLSKAAMAATPLVAIAIDLLVLRRRPR
                     RTIIAMLPWIILSLVAAFVARSFQSAAQVAHVSLPGRALVALDAIGFYVGKLLWPAQL
                     APDYGRNPNWVMHHLASAAIAAVGALVLLLLAISLAKRGRWWSAGIFIFVAGLSPYLG
                     LVPFDFQPVTTVADRYAYFGIIGVALLAALAARWRSGRILLVAALLIWSMLSWRQADR
                     WHDSQRLFEHTLSVNPDSIVAQTVLAFEARRDGRFNEAEQRYLRVLQIWPDDAAALFN
                     LGNLYLATNRLPQAVEMFDRATRALPSNAQMHNNYGVALARLGDRAAAARQFQAAIDL
                     NPDWPDPRRSLAKLRSTSTQP"
     gene            2903..4768
                     /locus_tag="VLI90_15345"
     CDS             2903..4768
                     /locus_tag="VLI90_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15635.1"
                     /translation="MLACFAALAWSAALTKNATIDEPTHTLSGWIELRYRDFRLEPVN
                     PSLWKCWAALPNLFTKLKVTFDAPVWKTPSFDPQGEIDWAGRTLYDTPGNDGDAFITR
                     TRAAMLLLGIALGALTGAWAYRLAGPLAAIVAVALFSFDPNFVAHAPLAKSDIAFGLV
                     LLALTRTVWRLGQRATIWRVITIGLLCGAAVGTKFSGVIVGPILAVMLLYRALMPLPW
                     DFLGHLIASRARRILAAAAVGFTAAALCWLVTWSAYDFRFRPAPAMDSRMDMATIQRR
                     VALFEASAADSSHRPTEEQVAHWPPSAVSRAVLFLDRHHLLPQAMLAGLWYQHACVTI
                     WPAFLMGEHYGLGRWYYFPLAMLFKTPLATLAAFAIAIVVAIVMRRKWPTRFSEPEAP
                     DESSAGSLKRVGLYPSSWLVACFVIPTGIFSIAALTTHLNIGVRNVLPLYPFFYIAVG
                     ATAAGASKHWPRATRLTLVALLIGLLAETLPAWPDYIAFFNTAAGGKLGGIQLLGDSN
                     LDWGQDFKPLAKWQQSHSDEQLFVDFYGTLDPRFYGLRYHPLGVAPDGSRPISDIPIP
                     NGAVLAISANHLQGLYLKPYERAFFAYIAQQRPIDVIGGSLYLYRWPPQPPPAPP"
     gene            complement(4707..>5024)
                     /locus_tag="VLI90_15350"
     CDS             complement(4707..>5024)
                     /locus_tag="VLI90_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV15636.1"
                     /translation="AAGDAPNAASASQVHNNWATALLAMDQPGEAFDHLRTAERLNPD
                     AFDPHYNLGQLLYRAGQYDAAVVELGKAVRIKPDHVGAQRALTAAQAAAAAASGTDTA
                     SPR"
BASE COUNT          839 a         1656 c         1612 g          917 t
ORIGIN      
        1 cgcttgcggt tttgcagacc agtgaccgcg gcttgtccgt ctgcgaaacc gcaagcgacc
       61 gccgcgccgt cttcccaaat catcccgctc attttgcctc tcgcccgccg ctccgctcgc
      121 tcttcagaaa tcgactcagg cccggtttga agatgacctt cccgtccacc accacgagtt
      181 cttcgtccag gaacagctga acggctttgc tcagcacatg ggcttccagc tccaggccct
      241 tgcgcttcac gtcgtccagc gagtcggcgc cgacggtgat gtggaagacg tcctgcatga
      301 tgaccgggcc ttcgtcgagt tgttcggtga cgaagtgcga cgtgcagcca tgcacgcgga
      361 cgccgctttc gtatgcctgc ttgtatggcg cggcgccggg aaagtatggc agcagcgacg
      421 ggtggatgtt gatgatgcga tagcggtagg tctgcacgag cgattccggc aggatgcgca
      481 tgtagcgggc gagcacgacc agatcggccg cggcggattt gagttgatcg gcgagccaat
      541 cgaaatgctt ctgcacgttg tcggccgcgg ggtgccaggc gaagggcagg ccggcttgtt
      601 tggcgatggg ttcgaggtcg ggatggttgg cgaagacgcc ggcgatttcg ccgtgcgctc
      661 ggccggtctt gaagtcatcg atcagcagct ccaggcagtg cggctctctg ctgaccagca
      721 gagcgacgcg cttcttgcgc ttctcgctgt gcaggtgggc cttcacttcc atatcgatct
      781 tgcggcccag ttccagcaac cccgtgatca gttctgacag gtcgatgttc aggtctttga
      841 gatcgaccag catgtccatg acgaagaacc ctcggacgac gcgctgttcg atgtcttcga
      901 tgttgatgcc acggtcggcc atgtaggtcg cgaactgcgc gacgacgccc ttctggtctt
      961 tgcccaggac gctaacgacc gcgagaaggg gttgtgctgc cacgcgggtg agtgtaaccg
     1021 cgcgcgggct tgagcgtaaa tgacggctca cggctgcgtg gatgtggatc gcagtttcgc
     1081 caggcttcgg cgcgggtcgg gccaatccgg attcagatca atcgccgcct ggaattgccg
     1141 ggcggcggcg gcgcgatcgc cgagacgcgc cagggcaacg ccgtaattgt tgtgcatctg
     1201 ggcgttcgac ggcaacgctc gggtcgcgcg gtcaaacatc tccactgcct gcggcaggcg
     1261 gttcgtcgcc agatataggt tgccaaggtt gaacagggcg gccgcatcgt ccggccagat
     1321 ctgcagcacg cggaggtaac gctgctcggc ttcgttgaag cggccgtctc gacgcgcttc
     1381 aaatgccagc acggtttggg cgacgatgct gtcgggattc accgacagcg tgtgctcgaa
     1441 caatcgctgg ctgtcgtgcc agcggtcggc ttgtcgccag ctcaacatcg accagatcaa
     1501 aagcgccgcg acgaggagga ttcgccccga ccgccatcga gcggcaaggg ccgcgagcag
     1561 cgcgacaccg attatgccga agtacgcgta gcgatcggct acggtcgtca cgggttgaaa
     1621 gtcgaatggc accaaaccga gatacggcga taggccggcg acgaagatga aaatgccggc
     1681 ggaccaccat cggccgcgct tcgcgagcga tatggccagt agaaggagga ccagcgctcc
     1741 caccgccgcg atcgccgcgg aggcgagatg gtgcatcacc cagttcgggt ttcgcccgta
     1801 gtccggcgcc agctgcgccg gccaaaggag cttcccgacg tagaagccga tggcatcgag
     1861 tgccaccagc gcccgccccg gcaacgacac gtgcgccacc tgcgccgccg actgaaacga
     1921 ccgcgcgacg aacgccgcca ccagcgacag gatgatccat ggcagcatgg cgatgatcgt
     1981 ccggcgcggg cgacggcgga ggacgagcag gtcgatggcg atagccacca gcggcgtcgc
     2041 cgccatcgcg gctttgctga gcagcgcggc gacgtacatc gcggtcgcta gcgcatatag
     2101 cgcaactcta ctcgcgcgcg aacgcgaccc gtcgccgctt ctgaggtaca cgatcatcgc
     2161 ccccagcgcc agcgccgccc cgagcaaggt gtacatgccg gacgcccagg cgatcgcttc
     2221 cgtttgcatc ggatgcacgg cgaacactgc cgcgccgaac gcggcggata tggtcgtcat
     2281 gcccagctgc cgcagcagga ggaacacgag caggacgcat gccgcgtgca gcgccaggtt
     2341 cagcgcgtgg aacggccacg ccgtcagcgt gccgtgcgcc tcccgcggcg agatcgcggc
     2401 aacgccgccc cacaccgtgt acgtcagcgg catgtaaagc gtcatccgcg tatgcgacca
     2461 gttccaggcc acgcggcgcg ccgtgaccgg attgaaatcc gggttctggt aaatctcggt
     2521 ctgatcgtcg aagtcaacaa attgatttcg cagcaccggc caataggtgc ccagcacgat
     2581 ccccagcagc accaggatgg ccacaggcaa atgcaggcgc tgccgcggtg cgttcattgc
     2641 cggcgattat gcgttggact cgcacgcgcg tcacgccggg ttcggaggta ctccgacgac
     2701 ccggatggat ttctcgcgcg ccagaagaat ccgggtcgtc gcgggtacgc tccgaacccg
     2761 gcgtgggtga aatcgcacgc gcacgcgcgc cggttgtcgt tcgtctcatg cggcgataga
     2821 ttgcccgctc ccgcgaatgg ctgacgctcc accgccccac cgagatcgaa ccccgcgcat
     2881 caccgccgct gtttgtggcc tgatgctcgc gtgcttcgcc gcgctggcgt ggtcggcggc
     2941 actgacgaag aacgcgacca tcgacgagcc gacccacacg ctcagcgggt ggatcgagct
     3001 gcgctatcgc gatttccgac tggaaccggt gaacccgtcg ctttggaaat gctgggcggc
     3061 gctgccaaac ctgttcacga aattgaaggt gacgttcgac gcgccggtct ggaagacgcc
     3121 gtcgtttgac ccgcagggcg aaatcgactg ggccgggcgg acgctgtacg acacgccggg
     3181 gaacgacgga gatgccttca tcacgcgaac acgcgcagcg atgttgctgc tgggcatcgc
     3241 gctgggcgcg ctgaccggcg cgtgggcgta cagattagcc ggcccactgg cggccatcgt
     3301 cgcggtcgcc ctgttcagct tcgatccgaa tttcgttgcg cacgcgccgc tggcaaagag
     3361 cgacatcgcc tttggcctgg tgttgctggc gctaacccgc accgtctggc ggctgggcca
     3421 acgcgcgacg atctggcgcg tcatcaccat cggcctccta tgcggcgccg ccgtgggcac
     3481 caagttctcc ggcgtcatcg tcggcccgat cctggcggtc atgctgctct atcgcgcgct
     3541 gatgcctctg ccgtgggatt tcctcggcca tctcatcgcc agccgcgctc gccgaatcct
     3601 cgccgcggct gccgtcgggt tcaccgcggc ggcactttgc tggctggtga cgtggtcggc
     3661 gtacgacttc cgcttccgcc cggcgccggc gatggattcg cggatggaca tggcgaccat
     3721 ccagcggcgg gtcgcgctgt tcgaagcctc cgcggccgat tcttcgcatc gaccgactga
     3781 ggaacaagtc gcgcactggc cgcccagcgc cgtctcgcgc gcggttttgt ttctcgatcg
     3841 ccatcacctg ctgccgcagg ccatgcttgc ggggctgtgg taccaacacg cgtgcgtgac
     3901 gatctggccg gcgttcttga tgggggagca ttacgggctg ggccggtggt attactttcc
     3961 gctggcgatg ctgttcaaaa caccgctcgc gacgctggcg gcattcgcga tcgcgatcgt
     4021 tgtcgcgatt gttatgcggc gcaagtggcc gactcgtttc agcgagcccg aagcaccgga
     4081 cgaatcgagc gccggctcgc tgaagcgagt cggcctctat ccttcatcct ggctcgtcgc
     4141 gtgcttcgtc attcccaccg gcatcttctc catcgcggcg ctcaccacgc acctgaacat
     4201 cggcgtgcgc aacgtcctgc cgctctaccc gttcttctac atcgcggtcg gcgccacggc
     4261 agccggcgca tccaagcatt ggccgcgcgc cacgcggcta acgctcgtcg cgctcctcat
     4321 cggactgctc gccgagacct tgccggcgtg gccggactac atcgcgttct ttaacaccgc
     4381 cgccggtgga aagctcggcg gcatccagtt gctgggcgac tcgaatctcg attggggaca
     4441 ggatttcaaa ccgctggcga agtggcaaca gtcgcattcc gacgagcaat tgttcgtgga
     4501 cttttacggc acgctcgacc cgcgctttta cggcctgcga tatcacccgc tcggcgtggc
     4561 cccggatgga tcgcggccga tctctgatat tcccattccc aacggcgccg tgctggccat
     4621 cagcgccaat catctgcagg gcctgtacct caagccgtac gagcgcgcgt tcttcgcgta
     4681 catcgcccag cagcggccga tcgatgtcat cgggggctcg ctgtatctgt accgctggcc
     4741 gccgcagccg ccgcctgcgc cgccgtgagc gcgcgctgcg cccccacatg atccggcttg
     4801 atgcgcaccg ctttgccgag ttcgaccacg gccgcatcgt attggcccgc gcgatacaac
     4861 agctgcccaa ggttgtagtg cggatcaaac gcatcggggt tcaatcgctc cgccgttcgc
     4921 aggtgatcga acgcctcgcc cggctggtcc atcgccagca gcgcggtcgc ccagttgttg
     4981 tgaacctgcg aagcggaggc tgcattcggc gcatcgcccg ccgc
//
LOCUS       DATIMH010000885         3199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1080124, whole genome shotgun sequence.
ACCESSION   DATIMH010000885 DATIMH010000000
VERSION     DATIMH010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080124"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VLI90_15355"
     CDS             <1..855
                     /locus_tag="VLI90_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15637.1"
                     /translation="VVWPWLIARNRAHFLVGAISAAALATLVTFVRRRNIRFVATGAV
                     GAALPLCMSLFHGSTAWWDCGFHFGTVHWPYMTMGLTDNLPGILAHRYGWTMEDLAAI
                     VTTIPAHAVHHWPAEPIDVTIRQLLLAIYGTMLLLSGIGLGLHARRRDRRVLAALVTP
                     WLMFFCFPPQIHERYLLFAAGAACLCAGVSVGMTLLGVFLSLVTFVMTLHCMMANNGG
                     QQNPQFDQWLAQQFPRFFDSGDAAGKVFRFFEGTHPDLGWAVLLCGGIFLYFTLAQRW
                     RATRAIQQ"
     gene            900..2357
                     /locus_tag="VLI90_15360"
     CDS             900..2357
                     /locus_tag="VLI90_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HSV15638.1"
                     /translation="MMRAGDSIPSTIDISLPQRRIAYAAAAILLGQAIWIGRAGFSPI
                     AMALITTAIALTAIGLFACDSPIVRRLPPGALRWLLIAGILAALARLPMIGLFVQFPR
                     SRATLMPFVALAMIAIVLIACIQLLRSRFLTAAAFGLLVAAHFLMGAWYLGISPDPHC
                     DVYLAQRLGCEELLQRRDPFAMTIPDIYGPTARFYPAGAVVNGQLQSGYFYPPLSLVL
                     DVPAYLAGDVRYAHLFALSLTALLIGFTRRDDGSSGVRFAPAIIVMFTPGIFWILENA
                     WIDPFLLLMLSATVCFASRGRFKLAAAAFGLFLVGKQYAPFTVLAVPLLLQAMRKAEP
                     RRPATSRLALASIAAGAVVTLPFFVWNPRAFLHSLISIYVGVLRPDSNTFLPLLARTF
                     NWHPSLALTTAVALTAGALVAWRAPRTLDGFAAGIAFILLCIFAFSTQAFGNYYFLAS
                     GALLAAIVAPAVGRDRFASDRRPMGRNATAEGAPR"
     gene            complement(2522..3067)
                     /locus_tag="VLI90_15365"
     CDS             complement(2522..3067)
                     /locus_tag="VLI90_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HSV15639.1"
                     /translation="MLRSVIITLCLILAGSLALAADDGSVTKAVANIKPSQAATTRPS
                     NNNITGTVTFSESGGKVTFVADIDGLSPGKHGFHIHKSSDLSAPDLSSAGGHFNPDKD
                     THGGPESPHHHMGDLGNLTADDKGHAHAEGTLPGATLGDGDHSILGKSVIVHAKEDDL
                     QTDPAGNSGGRMAGGVIEAAK"
BASE COUNT          454 a         1064 c         1000 g          681 t
ORIGIN      
        1 gttgtctggc cgtggttgat tgcgcgcaat cgcgcgcact tcctcgtcgg cgcgattagc
       61 gccgccgcgc tcgcgaccct cgttacgttc gttcgtcgcc ggaatattcg cttcgtcgcc
      121 accggcgctg tgggcgctgc gttgccgctg tgcatgtcgc tcttccacgg ctcaactgcg
      181 tggtgggatt gcgggtttca tttcggcacc gtgcattggc cgtacatgac aatgggcctg
      241 accgacaacc tgcccgggat tctcgcccat cgttatggct ggacgatgga agatctcgcc
      301 gcgatcgtca ccaccatccc cgcgcacgcg gttcaccatt ggccggcgga gccgatcgat
      361 gtcacgatcc gccaactgtt gctggcgatc tacgggacga tgctcctgct cagcggcatc
      421 ggccttgggc tgcacgcccg gcggcgcgat cgccgcgtgc tcgccgcgct ggtcacgccg
      481 tggttgatgt tcttctgctt tccgccgcag atccacgagc gttacctgct cttcgccgcc
      541 ggcgccgcgt gcctgtgcgc gggcgtcagc gtcggcatga cgctgctcgg cgtgtttctc
      601 agcctcgtca ctttcgtcat gacgctgcac tgcatgatgg cgaacaacgg cggccagcaa
      661 aatccacaat tcgaccagtg gcttgcgcag cagttccccc gattcttcga ctccggcgac
      721 gctgccggaa aagtctttcg attcttcgaa ggcacgcacc cagacctggg ctgggcggtg
      781 ctgctgtgcg gcgggatctt cctgtacttc acgctggcgc agcgatggcg cgccacacgt
      841 gcgatccaac agtagccccg catgtacatg cgcggcttct tcatttagct tgcgagcaca
      901 tgatgcgggc aggcgattcc atcccctcga cgatcgacat ttcgctgccg cagcgccgca
      961 ttgcatacgc ggccgcggcc atcttgctcg gacaagccat ttggatcggc cgcgccgggt
     1021 ttagcccgat cgcgatggcg ctgatcacca ccgcgattgc gcttacggcg atcggactct
     1081 tcgcgtgcga ttcaccgatc gttcgacgcc tgccgcccgg cgcgttgcgc tggctgctga
     1141 tcgccggcat cctcgccgcg ctggcgcggc tgccgatgat cgggctgttc gtgcagttcc
     1201 cacgctcgcg cgccacgctg atgccgttcg tcgcgctggc gatgatcgcg atcgtgctga
     1261 tcgcctgcat ccaactgctg cgttcccgct tcctcaccgc cgcggcgttc gggctgctgg
     1321 tcgcggcgca ctttctgatg ggggcgtggt atctcggcat ctcgccggac ccgcactgcg
     1381 acgtgtacct cgcgcagcgc ctgggatgtg aagaactgtt gcagcggcgc gatccgtttg
     1441 cgatgaccat tcccgacatc tacggtccaa cggcgcggtt ctaccccgcc ggcgcagtcg
     1501 tcaatgggca actgcagtcg ggatattttt atccgcccct gagcctcgtg ctcgacgtgc
     1561 ccgcctatct ggccggcgat gtgcgctatg cgcacctgtt cgcgctgtcg ctgaccgcgc
     1621 tgctcatcgg attcacacgg cgcgatgatg ggtcatcggg cgtgcgcttc gccccggcga
     1681 tcatcgtgat gttcaccccg ggcatcttct ggatactgga aaacgcttgg atcgacccgt
     1741 tcttgctgct gatgttatcg gcgacggttt gcttcgcgtc ccgcgggcgc ttcaagctcg
     1801 ccgccgcggc gtttggattg ttcctggtcg gcaaacaata cgcgccgttt accgttctgg
     1861 cggtaccatt gctgttacag gcgatgcgca aagccgagcc acggcgaccg gcaacgtccc
     1921 ggctggcgct ggcgagcatc gccgccgggg cggtcgtcac gctgccgttc ttcgtttgga
     1981 acccgcgcgc gtttcttcac agcctgatat cgatctacgt cggcgtcctg cggcccgact
     2041 ccaacacgtt cctcccgctg ctcgcaagga cgttcaactg gcacccgtcg ctggcgctga
     2101 ccaccgccgt ggcgctgacc gccggcgcgc tcgtcgcgtg gcgcgccccg cgcacgctcg
     2161 acggcttcgc cgccggaatc gccttcatcc tgctgtgcat cttcgcgttc agcacgcagg
     2221 cgttcggcaa ctattacttc ctggccagcg gggcactgct cgccgcgatc gtcgcgcctg
     2281 ctgttgggcg tgatcgattc gcaagcgatc ggcgtccaat ggggcgaaat gcaaccgccg
     2341 agggcgcgcc gagatgaacg ccgagaagag aaaactttga accgcagatt tcgcagattt
     2401 cgcagattca gaaaacgctt cttccaatct gcgtaatctg cggttcaaac tcttttgtct
     2461 tcgtcttctc ggcgtccgcc tcggcgccct cggcggttgc attgaggatc tgccaatcca
     2521 gtcacttcgc cgcttcgatc acgccgcccg ccatccggcc gccggagttg ccggcggggt
     2581 cggtctgcag atcgtcttcc ttcgcgtgca cgatgaccga tttgccgagg atcgagtgat
     2641 cgccgtcgcc gagcgtcgcg ccgggcaacg tgccttcggc gtgggcgtga cccttgtcgt
     2701 ccgccgtcag gttgcccagg tcgcccatgt gatggtgcgg cgattccggt ccgccgtgcg
     2761 tgtccttgtc cggattgaaa tgcccgcccg cgctggacag atccggcgcg ctcaggtcgc
     2821 tgcttttgtg gatgtgaaac ccatgcttgc cgggcgagag cccgtcgatg tcggcgacga
     2881 acgtgacctt cccaccggac tcgctgaacg tgacggtgcc ggtgatgttg ttgttgctcg
     2941 gccgcgtcgt cgccgcctgc gacggcttga tgttcgcgac cgccttggtg accgacccat
     3001 catccgcggc aagcgcgagg gaaccggcga ggatgaggca gagagtaatg atcacggaac
     3061 gcagcatggg cagctccttg tttgtggtgt gaatggaagt gagcagcatg ctacgccgcg
     3121 ggaccgctgc gcggcaagcg tcgcgcgtga acgttgcgca tggacaaaaa agtccacggc
     3181 catgccgagg gacggggtt
//
LOCUS       DATIMH010000886         5864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3388178, whole genome shotgun sequence.
ACCESSION   DATIMH010000886 DATIMH010000000
VERSION     DATIMH010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3388178"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /gene="gspD"
                     /locus_tag="VLI90_15370"
     CDS             <1..1976
                     /gene="gspD"
                     /locus_tag="VLI90_15370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HSV15640.1"
                     /translation="QVIPVRSVDAVKLKTDLQPLIGTEADIASNGGSNTIIITDTSAN
                     IHRVVEIVAAMDKRDSLENTIRVKQMKYADATAAAKLITDIFKTETQQGGGGGGNVPP
                     QVQFFRAFRGGGPGGGGPGGGGGGGNQNDQGNMGKVLASADQRTNTVVVTGPADTLKV
                     IDDVLSQLDSNPSAEQTFFLYRLKNGQAQDMQATLNSLFGTGTGTSANRTGTSTSGIR
                     TTGTNSFGGGGGGFGGGGFGGSGGGLGGGGLGGRGGTSGFGGGGGGGFGGGGFGGGGF
                     GGGGLGGAGQFGRTATGGAGGAGGSSAMTDLLGQVYVVADQDTNSLLVATASKYEDRV
                     KSIIQELDRPVPQVLIKVLIAEVTHDNSSDLGMDFSVLNTRASGNGQRIGSSFGNAAA
                     GAANGGLVVSMLESQVQATLHALATSGKLDVLSRPYILASDNQQASITVGQIVPFVTE
                     SRLDQNSNTINTIQYQDIGIILNVTPHINPDGLVILDVAPQISSLTQTTIQISAGVSA
                     PVFQLRSAQSRVGIKDNQTIVIGGLMEDRKNSTLEKVPILGDIPLLGLAFQRNQVTKT
                     KTELLIFLTPHVAAAPEQLKPMSADEMKGLKLTPQAVQPGTFQDHMEGMQRGGGAPAE
                     QPDRGKAYDTAPGTKNDQQGAPTPPPSNQEENQ"
     gene            2152..3054
                     /locus_tag="VLI90_15375"
     CDS             2152..3054
                     /locus_tag="VLI90_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSV15641.1"
                     /translation="MISRRLWNLQYRLAPYLFVAPFVLLFLVFLLYPLIRSLILSFCN
                     AVGPQEIYFTGSRNYVHLIRDPYFWMAAANTTLYVLAFLLIQVPLSLGLAILLNNPVV
                     RWRTIFRFALISTHLVGHVFVAVVFSQLLDPRHGPVTRLVAAVTGGSIEIPWLTNPLL
                     ARASVLMAWLWMSVGYGMIYLLAALQAVDQNLYEAAEMDGATGWSRFWHVTLPGIRPV
                     LTFLILVGLIGGFQLFELPFVLLQGAGPGSAGLTIVMYLFNTGFLAGNLGYASTIGWA
                     LVAILIVVSVLQLWLTRAAATEEH"
     gene            3057..3914
                     /locus_tag="VLI90_15380"
     CDS             3057..3914
                     /locus_tag="VLI90_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSV15642.1"
                     /translation="MWRARQSRWTIVPLHLLLILAAAISLAPFAWLICASLKTGGDLF
                     HYAFLPISRRGVLISHLTLSNYRRLFAENPFARWMINSIFLACAQTVISVTLSSLGGF
                     ALAKYRFAGQRLIMLIMLGTMLLPAQVLMPSSYELMYKFGWLDSYLAILVPGAVSVFG
                     LILFRQAMLAVPDELLHAARIDGCNEIHLWWQIALPLVRPMIGAFTLLSFTATWNSFL
                     WPQIVLQNQAKYTLPIGLYNLNGMPGFQTDFGVLMAATLLSVLPLVVLFFLLQRDFIA
                     GLTAGAMKG"
     gene            complement(4212..4988)
                     /locus_tag="VLI90_15385"
     CDS             complement(4212..4988)
                     /locus_tag="VLI90_15385"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSV15643.1"
                     /translation="MMLMGCADKLVLYPSTHALDARGAKRRTISFGDGVLEVLVARSP
                     GCAKSGEPVAFDLEFVGNGTRAEWVAGPVAQRWGDRPVEVWALNYPGYGRSTGPASLR
                     SIPPAALATYDAMEKAAAGRPIFVGSHSLGTTVALYVATQRPVKGMILLNPPPLRELI
                     LQHYGWWNLWVAASAVAADIPAELDSLTNAAKGTALAIFLSAALDAVVPPKFHRMVYD
                     AYGGTKRLIELPDAEHNTVPDPRTLDELRLGMDWLWPHHH"
     gene            5226..>5864
                     /locus_tag="VLI90_15390"
     CDS             5226..>5864
                     /locus_tag="VLI90_15390"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HSV15644.1"
                     /translation="MPAFTYEPTFQTGADETPYRQLSGEHVTTVEIAGRTFLKIEPEA
                     LRLLARQAFTDVSFYLRPGHLRQLREELADPEASDNDRFVLYTHLQNAVVAAAGRLPS
                     CQDTGTAIVLGKKGQYVLSDGDDAEALSRGIFETYQERNLRYSQIAPLEMFKERNTAN
                     NLPAQIDLLAEPGGDYKFLFIAKGGGSANKAFLFQSTPAVLNEKDLETFVRSK"
BASE COUNT         1054 a         1854 c         1909 g         1047 t
ORIGIN      
        1 cgcaggtgat ccccgtgcgc agcgtggacg cggtgaagct caagaccgac ctgcagccgc
       61 tgatcggcac cgaggcggac atcgcatcca acggcggaag caacacgatc atcatcaccg
      121 acacctcggc gaacattcat cgcgtggtcg agatcgtggc ggcgatggac aagcgcgatt
      181 cgctggagaa cacgatccgc gtgaagcaga tgaagtacgc cgacgccacc gcggcggcga
      241 agctgatcac ggacatcttc aagaccgaaa cgcaacaagg cggtggtggt ggcgggaacg
      301 tgccgccgca ggtgcagttc ttccgcgcct tccgcggtgg tggacccggc ggcggtggtc
      361 ccggcggtgg tggcggcggt ggaaatcaga acgatcaggg gaacatggga aaggtgttgg
      421 catcggcgga ccagcggacg aacaccgtcg tcgtcaccgg gccggctgac acgttgaagg
      481 ttatcgatga tgtcctgagc caactcgatt ccaatccgtc ggcggagcag acgttcttcc
      541 tctatcgcct gaaaaatggt caggcgcagg acatgcaggc gacgctgaac agcttgttcg
      601 gcaccggcac gggaaccagc gccaaccgca ccggcacgag caccagcggc atccgcacga
      661 ccggcaccaa ttccttcggc ggcggtggcg gtgggtttgg cggcggtgga tttggtggca
      721 gcggcggtgg gcttggcggt ggaggactcg gtggccgcgg gggcaccagt gggttcggcg
      781 gcggcggtgg tggtggcttt ggaggaggcg ggttcggtgg cggtggattc ggtggtggcg
      841 ggttgggtgg cgcggggcag ttcggccgca ccgccacggg tggcgcaggc ggcgccggtg
      901 gcagcagcgc gatgacggat ctgctcgggc aggtttacgt cgtcgccgac caggacacga
      961 actcgctgct ggtcgccacg gccagtaagt acgaagatcg cgtgaagtcg atcatccagg
     1021 aactggatcg cccggtgccg caggtgctga tcaaagtgct gatcgccgaa gtgacgcacg
     1081 ataacagctc ggacctgggc atggatttct ccgtgctcaa cacgcgcgcc agcgggaacg
     1141 gccagaggat cggttcctcg ttcggcaacg ccgccgcggg cgctgccaac ggcgggctcg
     1201 tcgtgagcat gttggaaagc caggtgcagg caacgctgca cgcgctggcg accagcggga
     1261 agctggacgt gctgtcgcgg ccatacatcc tggccagcga caaccagcag gcgtcgatca
     1321 cggtcgggca gatcgtgccg ttcgtcaccg aaagccggct cgatcagaac agcaacacga
     1381 tcaacacgat ccagtaccag gacatcggca tcattctgaa cgtgacgccg cacatcaacc
     1441 ctgacgggct ggtgattctg gacgtggcgc cgcagatttc gtcgctgacg cagacgacga
     1501 tccagatcag cgccggcgtg agcgcgccgg tgttccagct gcgcagcgcg caaagccgcg
     1561 ttggtattaa agacaatcaa acgattgtga tcggcgggtt gatggaagac cggaagaact
     1621 cgacgctgga gaaggtgccg atcctggggg acatcccgct gctggggctc gcgttccagc
     1681 ggaaccaggt gaccaagacg aagaccgagc tgctgatctt cctcacgccg cacgtggcgg
     1741 cggcgccgga gcagctgaag ccgatgtcgg ccgacgaaat gaagggcctg aagctgaccc
     1801 cgcaggcggt gcaaccgggg acgttccagg accacatgga aggcatgcag cgcggcggcg
     1861 gcgcgccggc cgagcagccg gatcgcggca aggcatacga caccgcgccg gggacgaaga
     1921 acgaccagca aggcgcgccc acaccgccgc cgtcgaatca ggaggagaat cagtagcgtt
     1981 tgacgcggtg gacgaacgcc agagccgcgc acgaagtacg cgggaacgaa gcaccgctta
     2041 ccgcgtgcgc ggctctggtc gtttcgggtc cgtcatggtg aaatcatcct cacccggcgg
     2101 ctcggcgggc ggaaccgccg ggtcacgaac gggcattgag gaccgaaggg cttgatctct
     2161 cgccgcctct ggaacctgca atatcgcctt gccccctacc tcttcgttgc gccgttcgtg
     2221 ctgctgttcc tcgtcttcct gctttacccg ctgatccgca gcctgatcct cagcttctgc
     2281 aacgcggtgg gcccgcagga aatttacttc acgggctcgc gcaattacgt ccacctgatt
     2341 cgcgatccat atttctggat ggccgcggcg aacacgacgc tctacgtgct ggcgttcctg
     2401 ctgatccagg tcccgctgtc gctggggctg gcgattctgc tgaacaatcc agttgtgcgc
     2461 tggcgcacga tctttcgctt cgcgttgatc tcgacgcatc tcgtcgggca cgtcttcgtc
     2521 gcggtcgtat tttcgcaact gctcgacccg cggcacgggc cggtcacgcg gttggtcgcc
     2581 gccgtcactg gtggatccat tgagataccc tggctgacaa atccactgct ggcgcgcgcc
     2641 tcggtgctga tggcgtggct gtggatgtcg gtcggctacg ggatgatcta tctgctggct
     2701 gcgttgcaag ccgtcgatca gaacctgtac gaagccgccg agatggacgg ggccaccggg
     2761 tggtcgcgct tctggcacgt cacgctgccg ggcattcgcc cggtgctgac gttcctgatc
     2821 cttgtcggat tgatcggcgg atttcagctg ttcgagttgc ccttcgtgct gctgcaaggc
     2881 gccggtcccg ggtcggccgg gttaaccatc gtcatgtact tgttcaacac cggcttcctc
     2941 gccggcaatt tgggttacgc atcgacgatt ggatgggcgc tggtcgcgat cctgatcgtc
     3001 gtcagcgttc tgcaactgtg gctgacgcgc gccgcggcca cggaggaaca ttaatcgtgt
     3061 ggcgcgcccg ccaaagccga tggacgatcg tcccgctgca cctgctgctg atcctcgcgg
     3121 cggcgatcag cctcgcgccg ttcgcgtggc tcatctgcgc ctctctgaaa accggcggcg
     3181 acctgttcca ctacgcgttc ctccccatct cccgccgcgg ggtgctgatc agccatctca
     3241 cgctatcgaa ctaccgccgg ctcttcgctg agaacccctt cgcgcgctgg atgatcaact
     3301 cgatcttcct cgcgtgcgcg cagaccgtga tctcggtgac gctcagcagc ctcggtggat
     3361 ttgcgctggc gaagtatcgt tttgccggcc agcggctgat catgctgatc atgctgggca
     3421 cgatgctgct gccggcgcag gtgttgatgc ccagcagcta cgagctgatg tacaagttcg
     3481 gctggctcga ttcctatctc gcgatcctcg tccccggcgc ggtcagcgtc ttcggtctga
     3541 tcctgttccg ccaggcgatg ctcgcggtgc ccgatgaatt gctgcacgcg gcgcgcatcg
     3601 acggctgcaa tgaaatccac ctctggtggc agattgccct gccgctggtg cggccgatga
     3661 tcggcgcgtt cacgctgctc agcttcaccg cgacgtggaa cagttttctc tggccgcaga
     3721 tcgtcctgca gaaccaggcg aagtacacgc tgccgatcgg cctgtacaac ctgaacggca
     3781 tgccaggttt tcaaacggac ttcggcgtgc tgatggcggc gacgctcctg agcgtcctgc
     3841 cgctggtggt gctgttcttc ctgctacagc gcgatttcat cgcgggcctg accgcgggcg
     3901 cgatgaaggg gtgagtcgcg gtgtgggcgc gcggcgtgcg cgcgcttgtc atctccagcg
     3961 caggcgtact cgccggagtc gaaggatcta gcgaagaagg gatgggacga tccgatagcg
     4021 cactcactca tcgacgtccg aggcaagatc cttcgactgc gcacgagtac gcgctccgct
     4081 ggaggatgac aaatatcctt catagccccg gccggaaggc cggggatcgg actgcaaatt
     4141 ggcgatgcta tccgtccgac ccccggcctt ccggccgggg ctattaaagc catcgtactt
     4201 cccgacaggc tctaatgatg atgcggccag agccaatcca tccccagccg cagttcatcc
     4261 aacgtccgcg ggtccggcac cgtgttgtgc tccgcatccg gcagctcgat caaacgcttc
     4321 gttccgccat acgcgtcgta gaccatccgg tgaaacttcg gcggcaccac cgcatcgagc
     4381 gccgcggaca gaaagatcgc cagcgccgtc cccttcgccg cgttggtcag gctgtccaac
     4441 tccgccggga tatccgccgc gacggcgctg gcggccaccc ataggttcca ccacccgtaa
     4501 tgctgcagga tcagctcgcg cagcggcggc ggatttagca ggatcattcc cttcaccggc
     4561 cgctgcgtcg caacgtaaag cgcgaccgtc gttccgagac tgtgcgagcc gacgaagatc
     4621 ggtcggcccg cggccgcctt ttccatcgcg tcgtacgtcg ccagcgccgc cggcgggatc
     4681 gaccgcaacg atgccggtcc ggtgcttcga ccatagccgg gataattcag cgcccacact
     4741 tcgaccggcc gatcgcccca gcgctgggct acaggccccg ccacccattc ggcccgcgtg
     4801 ccgttgccga cgaactccag atcgaacgcc accggctcgc cggacttcgc gcaaccgggc
     4861 gaccgcgcga ccaacacttc cagaactcca tcgccaaagg aaatcgtccg gcgcttcgcc
     4921 ccgcgcgcat ccagcgcatg cgtcgacggg taaagcacga gtttgtcagc acaacccatc
     4981 agcatcatga acgtcaaagc gacggatatt tttagacgcg ccgcagccgc aaagcgaccg
     5041 ccctcaggcg tgggcagcat cgcgatatgg gctttcgaaa tcgccaatca caactccagc
     5101 gcggggcaat cggctatggc tccttccccc tgtccaggcg taccgggcga gggcgaaaga
     5161 gcgacacgcg atcgcccggt cgtctcgcgt ggacgctaac gcggcggccc gttaatcttg
     5221 ggcaaatgcc agcattcacg tatgaaccga cgttccaaac cggcgccgac gagacgccgt
     5281 atcgacagct gagcggcgag cacgttacga ccgtcgaaat cgccggccgc acgtttttga
     5341 agatcgagcc cgaggcgctg cgcctgctcg cgcggcaggc gttcaccgat gtctcgttct
     5401 atcttcgccc cggtcatctt cggcagctgc gcgaagagtt ggccgatcct gaagccagcg
     5461 ataacgatcg cttcgtgctg tacacgcacc tgcagaatgc cgtcgtcgcg gcggcgggtc
     5521 ggttgccaag ttgccaggac acgggcacgg cgatcgtgtt ggggaagaag gggcaatatg
     5581 tgctgagtga cggcgacgat gccgaggcgc tgagccgggg gattttcgag acgtatcagg
     5641 aacgcaacct gcgctattcg cagatcgcgc cgctggagat gttcaaggaa aggaacacgg
     5701 cgaacaacct gccggcgcag atcgacctgc tggccgagcc gggcggcgac tacaagttcc
     5761 tcttcatcgc caagggcggg gggagcgcga acaaggcgtt tctattccag tcgacgccgg
     5821 cggtgttgaa cgagaaggac ctggagacgt tcgtgcggtc gaag
//
LOCUS       DATIMH010000887         3973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3101045, whole genome shotgun sequence.
ACCESSION   DATIMH010000887 DATIMH010000000
VERSION     DATIMH010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3101045"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..543)
                     /locus_tag="VLI90_15395"
     CDS             complement(46..543)
                     /locus_tag="VLI90_15395"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HSV15645.1"
                     /translation="MRREADRNVRPHQLGKRKRESSQYFIISTSFGMPRYTPVAAAAI
                     GLGPRVRGFHFTREGWQMKYIVSWFERPQGSPIEYENAQKRILEVFGQWKAPANFKIE
                     FFVIRVGEWGGHMLLDCDDLVTIHKFCSMLPAFVFQVHPVIPVEEAVRGELEVMAWRD
                     GLKRK"
     gene            complement(639..1322)
                     /locus_tag="VLI90_15400"
     CDS             complement(639..1322)
                     /locus_tag="VLI90_15400"
                     /inference="COORDINATES: protein motif:HMM:NF025718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HSV15646.1"
                     /translation="MMNLLAITLTPWPVIGVRFALVVAGIGLWYLTQALLAKRVPKVA
                     YEIPLTDGIHVLTARIHKRYFTNIAAGNRLLIASSLVIDLLGAYLIGSAIFGVSIRPY
                     LGLVMVFALRQVCQMLCPLPAPHEMVWRYPGVPAVLVTYGTANDLFFSGHTAIAVYGA
                     ATLAGHWGHAGLVLGIAIALFEAVTVLVLRAHYTMDVFTGIICALWVYSLSGPLALWV
                     DGAIGQWVG"
     gene            complement(1461..2186)
                     /locus_tag="VLI90_15405"
     CDS             complement(1461..2186)
                     /locus_tag="VLI90_15405"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSV15647.1"
                     /translation="MYRPGKTKRFDFPTGRSVAGKYVIERPLGSGWEGEVYAIIEKNT
                     GIRRAAKFYYPHRDPTGRAAIAYARKLDALRHCPILMQYHHQEMAYVRKKKVTVVISE
                     LVEGKKLSEFLAENPGHRLSTFEALHVLYILARGIAPIHARGEYHGDIHDDNIMIRRQ
                     GISFDVKLLDFFDLGAPARSKIRKDVLNLVEVFHTTVGGRKHYAEQPKVVKDIIRGLK
                     DSLILARFQSAGDIARHLEALEW"
     gene            2348..2740
                     /locus_tag="VLI90_15410"
     CDS             2348..2740
                     /locus_tag="VLI90_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15648.1"
                     /translation="MDFLKTVGGKIAGGLVALAVIAAAISWWQTDPATRHAIVSAAGR
                     LTGWSLSVLALPWLCFWIVGWVARMDSNFAGAALVFILTALEAVALAWLFGWSIHGAT
                     GWIFYIAAVLVAAVYNLFTCDWIAEQIE"
     gene            2851..3897
                     /locus_tag="VLI90_15415"
     CDS             2851..3897
                     /locus_tag="VLI90_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSV15649.1"
                     /translation="MASANQRVGEYVLEQKVGGGTFGEVWRAHHHVWSDQLVAVKIPT
                     NPQYLRDLQREGCAIHGLIHPNIVRAMGFDPYAETPYLTMEYIAGSSLRPLIQKRSIN
                     INDTAAIMRQVLAGLGYAHKNGIIHRDIKPENILVHERAFTDGFATEGVVKVTDFGLG
                     KAATNNVVGSIAYSASLNDAAGREIAGSLDYMAPEQRGGGAVDARADLYACGVVLYEL
                     LTGEKPAGTDVPSDVNKAVPKIYDEIFRRSYARLEKRFASAEEFAQALPAANGQSRPF
                     QRPAPASANRPRTVSCPRCNRPVDAGDQFCLHCGVQMVTTIRRCQKCGSYPDASDRYC
                     IFCGEALTPELSTV"
BASE COUNT          756 a         1182 c         1285 g          750 t
ORIGIN      
        1 gcgcgtgtgt ggagcttcac gaaatggaat ttgcgccgcg cccgattact tacgcttcaa
       61 cccatcgcgc cacgccatca cttcaagctc gccgcgaacg gcttcttcga ccggaatgac
      121 cggatgcact tggaatacga acgcgggcag catcgagcag aacttgtgaa tggtcaccag
      181 gtcgtcgcaa tccagcagca tgtgcccacc ccattcgccg acgcggatga cgaagaattc
      241 gatcttgaag ttggcggggg ccttccattg cccgaagact tcgaggattc gcttctgcgc
      301 gttttcgtat tcgatgggtg aaccctgcgg ccgctcgaac cagctgacga tgtacttcat
      361 ttgccatcct tcccgagtga aatgaaagcc cctgacgcgc ggccccaagc cgatggctgc
      421 ggccgcgacg ggagtataac gcggcatgcc gaatgatgtt gaaataatga aatattgcga
      481 ggattctcgc ttgcgctttc caagctggtg ggggcggaca ttcctgtcgg cctcgcggcg
      541 caccgttcgc cctgcatggc cgcgaaagcg tgcggagtga agcgctcagc agcgccagaa
      601 gcatccgcgc ttcgccattt gttccatggc aacgcgtttt atccaaccca ttggccgatg
      661 gcgccatcca cccagagggc cagcggaccg ctgagggaat agacccacag cgcgcagatg
      721 atgccggtga agacgtccat cgtgtagtgc gcgcgaagca ccagcacggt gacggcttcg
      781 aacagtgcga tggcgatgcc gagcaccagc cccgcgtggc cccagtggcc ggctaacgtc
      841 gcggcgccgt aaaccgcgat cgcggtgtgc ccggagaaga acaggtcgtt ggcggtgccg
      901 taggtgacga ggaccgcggg cacgccgggg tatcgccaga ccatctcgtg cggcgccggc
      961 agggggcaaa gcatctggca gacctgccgg agcgcgaaga ccatcaccag cccaaggtac
     1021 ggtcggatgg acacgccgaa gatcgcgctg ccgatcaggt acgcgcccag cagatcgatc
     1081 accagcgacg acgcgatcag cagccggttc cccgccgcga tgttcgtgaa atatcgcttg
     1141 tgaatccgcg cggtgagcac gtggatgcca tcggtcagcg ggatttcata tgcgaccttc
     1201 ggcacccgct tggcgagcag cgcctgcgtc agataccaaa gcccaatccc cgcgacgacc
     1261 aaggcgaaac gaacgccgat caccggccag ggagtgagcg ttatcgcgag aaggttcatc
     1321 atgtttattt aatccctgac cagaagaacg tgtgtgtgcg tccttgtcat cctgcagcgc
     1381 aggcgtgctc gccggagtcc aaggatgtgg ccggcgcgcc agtgaatgag cactcgcgca
     1441 accgactgcc gggcaccgct tcaccactcc agcgcttcta aatgccgcgc gatgtcgccg
     1501 gcgctttgga acctcgccag gatcagcgaa tccttcagcc cgcggatgat gtccttcacc
     1561 accttcggct gttccgcata gtgcttgcgc ccgcccaccg tcgtgtgaaa cacctcgacc
     1621 aggttcagca cgtccttgcg gatcttgctg cgcgccggcg cgcccaggtc gaagaagtcc
     1681 agcagcttca cgtcgaagct gatgccttgc cggcggatca tgatgttgtc gtcgtggatg
     1741 tcgccgtggt attcgccgcg agcatggatc ggggcgatgc cgcgcgccag gatgtacagc
     1801 acatgcagcg cctcgaacgt gctgaggcga tggccggggt tctcggcgag aaactcgctg
     1861 agctttttgc cttcaaccag ttcgctgatg accacggtca cttttttctt gcggacgtac
     1921 gccatctcct gatggtgata ttgcatcagg atcgggcagt gccgcagggc gtcgagcttg
     1981 cgggcgtacg cgatggccgc ccggccggtg gggtcgcggt gcggatagta aaacttggcg
     2041 gcccggcgaa tgccggtgtt cttctcgatg atcgcgtaaa cttccccttc ccaaccgctg
     2101 cccagcgggc gttcgatgac gtattttccc gcaacgctcc gcccggtggg aaaatcgaaa
     2161 cgcttggtct tgccgggccg gtacatgggg gcaagcgtag ggcggcggcg gggcgcgtca
     2221 atcgcgccgt tgcccggaaa aatcggtggt tcaggacggg cggcattagc ttgggaaatg
     2281 gccgcggttt ataacggcag gggggcctgg ccttgttaca attcacggca agatatgggg
     2341 atcaggcatg gacttcctca agacggtggg cggaaaaatc gccggtggac tggtggcctt
     2401 ggccgtcatc gccgctgcca tttcatggtg gcagaccgac ccggccacgc gccacgccat
     2461 cgtctccgcc gccgggcgat tgaccgggtg gagcctcagc gtcctggcgt tgccgtggct
     2521 ctgtttctgg atcgtcggtt gggtcgcgcg gatggacagc aacttcgcgg gggcagcgct
     2581 cgtgtttatc ttgacggccc tggaagcggt cgcgctggcg tggctgttcg gatggtcgat
     2641 ccacggcgcc accggctgga tcttctatat cgccgcggtg ctggtcgccg cggtatacaa
     2701 tctgttcacg tgcgactgga ttgccgagca gattgagtga aagagtgatg gcgtgattac
     2761 gtgaatacgt gaatacgtga gtacgtgaat acgtgatgaa gatgatcgtt tctttaccgt
     2821 gatcacgtat tcacgagacg gagcgaacga atggcaagcg ccaatcaacg cgtcggtgaa
     2881 tatgtgctgg agcagaaggt cggcggcggc accttcggcg aggtctggcg cgcccatcat
     2941 cacgtgtgga gcgaccagct ggtggccgtg aagattccga ccaacccgca gtacctgcgc
     3001 gatctgcaac gtgaagggtg cgcgatccac ggcttgatcc atccgaacat cgtccgcgcg
     3061 atgggcttcg atccgtacgc ggagacgccg tacctgacga tggaatatat cgccggctcg
     3121 agcctgcggc cgctgattca gaagcgatcg atcaacatca acgacaccgc cgccatcatg
     3181 cggcaggtgc tggccggtct tggttacgcg cacaagaacg ggatcatcca tcgcgatatc
     3241 aagccggaga acatcctcgt tcacgagcgc gcattcacgg atggatttgc caccgagggt
     3301 gtcgtgaagg tgacggattt tggactgggt aaagccgcga cgaacaacgt tgtcggttcc
     3361 atcgcgtact cggcatcgct gaacgacgcg gcggggcgcg agatcgccgg gtcgctggac
     3421 tacatggccc ccgaacaacg cggcggcggc gcggttgacg cgcgggcgga cctctacgcc
     3481 tgcggcgtcg tgctgtacga actgctgacc ggcgaaaaac ccgccggcac ggatgtgccg
     3541 agcgacgtga acaaggcggt gccgaagatt tacgacgaga tcttccgccg gtcgtatgcg
     3601 cgattagaaa agcgcttcgc gtcagcggaa gagttcgcgc aggcgttgcc cgccgcgaat
     3661 ggacaatcgc ggccattcca gcggccggcg ccggcgagcg cgaatcggcc gcgcaccgtc
     3721 agctgtccgc gctgcaatcg gccggtcgat gccggcgatc agttctgcct gcactgtggc
     3781 gtgcagatgg tgacgacgat ccgccgctgc caaaaatgtg ggtcgtatcc ggatgcgtcg
     3841 gaccggtact gtatcttctg tggtgaagca ctgacgccgg agctatcgac ggtgtgactc
     3901 ggtgccgctt gcggcttagc atctgttcgt cggaaaaaca gacgctgggc cgaaagcggc
     3961 tcccgagcag gaa
//
LOCUS       DATIMH010000888         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_793617, whole genome shotgun sequence.
ACCESSION   DATIMH010000888 DATIMH010000000
VERSION     DATIMH010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793617"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VLI90_15420"
     CDS             <1..864
                     /locus_tag="VLI90_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15650.1"
                     /translation="VGARRGVVLSSTNGAGTSRFILSGPGTGALIPDGAGGFSLTLTG
                     TTAASNVIILAPPGGVMLDAITADAPVGLLVGRAASVNGNVTLPGGARTVLLANVSNA
                     SMTFAGATPMVLSLRDVTDSSIVSNGPLNALLVNSWTNTGNPRSIAAPSIRVLLDKGN
                     FAGDIAAQSITTAIIKGDLTGDILAGAAFGPDNEPGGGDDTFAAGSIGTLFVSGSVNS
                     SLIAAGLNVIDDAFPLSDNDSLIAGSSIRNVVVSGTLSDDSRVLAATLPARARLGNTS
                     VVTASDPRFVL"
     gene            1128..1781
                     /locus_tag="VLI90_15425"
     CDS             1128..1781
                     /locus_tag="VLI90_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15651.1"
                     /translation="MSNPRGRKRSPISGLIAVVVVITTLVVLAAVRVGSLFRPKYVPI
                     EQLKFPVLVLQPDGMALFAEWDSTALQKFPERSMRTPVNGTVLIDSAFAQFTQENVKR
                     QSEGELKWIARALVPGLRVKYSFDLRRRADASGRERLAAIIARSPRFSDDPAEDAAMR
                     AAALQQTTMDGVLDALRLYKPAEPATPAAAVELGEPMVVDQSTPTTTEVEPAKPDGN"
     gene            complement(2017..>2584)
                     /locus_tag="VLI90_15430"
     CDS             complement(2017..>2584)
                     /locus_tag="VLI90_15430"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HSV15652.1"
                     /translation="RKFDDFADGGGQLVLTCRSGLMDRNGQLWEGPLAKPIVPLMGAT
                     ITGYDGLPDDSHGTVAFAHKTFQWNVWADLLAPEKETEVLAKYTDQFYTGTAAITRRK
                     HGEGAVTYCGVYGDASLADALVEKVVGDARIGAAPLPQRLRVLRRGPYTIALNYRDIA
                     TPAPAPKDAKFLIGTRTIEPAGVAVWET"
BASE COUNT          420 a          857 c          856 g          451 t
ORIGIN      
        1 gtcggcgccc gtcgcggcgt cgtcctgagc agcaccaacg gcgctgggac gagccgattt
       61 atcctcagcg gccccggcac cggcgcgttg atccccgacg gtgcgggcgg cttttcgctc
      121 accctcaccg gcaccaccgc cgcgtccaac gtaatcattc tcgccccccc cggcggcgtg
      181 atgcttgatg cgatcaccgc cgacgcgccg gtcggtttgc tcgttggtcg cgccgcttcg
      241 gtcaatggca acgtcacgct gccgggtggc gctcgcaccg tcttgctggc caatgtcagc
      301 aacgcatcga tgaccttcgc cggcgcaacg ccgatggtgc tgtcgctgcg cgatgtcacc
      361 gattcgtcga tcgtctccaa cgggccgctc aacgcgctcc tcgtgaattc ctggaccaac
      421 accggcaacc cgcgcagcat cgccgccccg tcgatccgcg tgctgctcga caagggaaac
      481 ttcgccggcg acatcgcggc ccagtccatc accaccgcga tcatcaaagg cgacctgacc
      541 ggtgacatcc tcgccggcgc cgccttcggt cccgacaacg agcccggcgg cggtgacgac
      601 accttcgccg ccggcagcat tggcaccctg ttcgtctccg gctcggtcaa cagctcgctc
      661 atcgccgccg gtttgaacgt gatagacgac gcgttccccc tcagcgacaa cgactcgctg
      721 atcgccggct cgagcatccg caacgtcgtt gtcagcggca cgctcagcga cgacagccgc
      781 gtcctcgccg ccacgctgcc ggcgcgggcg cgtctgggga acacttcggt cgtgaccgcg
      841 agcgacccgc gattcgtact gtgattcagc cggttgcggc ttcgccatcc acgccgcccg
      901 gtctgcaatg ccgcaagcgg ctaatgctgc cgcggccagt gagtcctgca gacgctggcc
      961 ttagccatat cgtcgaacgg agaccgtcat gcaggccgtg gtggaccagt ttcgaatgac
     1021 gccgctgttt acgtagcgga cgcgcgcgaa ttgtgcacga tgacttccca ggcgcgcccg
     1081 ggcgcgcgcg ttcgcatccg tgcatccagt tcgttccaag gaacttcatg tccaatccac
     1141 gcgggcgaaa acgctcgccg atttcggggc tcatcgcggt ggtcgtcgtc atcacgacgc
     1201 tggtggtact ggcggcggtg cgggtggggt cgctgtttcg gccgaaatat gtgccgatcg
     1261 agcagctgaa gtttccggtg ctggtgctgc agccggatgg catggcgttg ttcgcggagt
     1321 gggattcgac ggcgctgcag aagtttcccg agcggtcgat gcgcacgccg gtgaatggga
     1381 cggtcctcat tgattcggcg ttcgcgcagt tcacgcagga gaacgtgaag cggcagagcg
     1441 agggcgagtt gaaatggatc gcccgcgcgc tggtgcccgg ccttcgcgtg aagtacagct
     1501 tcgacttgcg ccggcgcgcc gacgcatcag gccgcgagag gctggcggcg atcatcgcgc
     1561 gatctccccg attcagtgat gatccggcgg aagatgccgc gatgcgggcg gcggccctgc
     1621 agcaaacgac gatggacggc gtgctggatg cgctgcggtt atacaagccg gcggagccgg
     1681 cgacgccggc ggcggcggtg gaactggggg agccaatggt cgtggatcaa tcaacgccaa
     1741 cgacgacgga agtggaaccg gcgaagccgg acgggaattg aaaacattcg gaccagccgt
     1801 cttcgaaaac aattcgttca ccgactactc ggcaagatcc ttcgactctg gcgagtacgc
     1861 ccgcgctgca ggatgacgag cgcgcaaaac tcgcgattgc gacgggaacg ctgctgagcg
     1921 cgcgaagacc gggccgggga cggaccggat ctccttggcg gcggtcgtgc acggggagag
     1981 ccgggccgtc ctcggcccgg tcttcgcccg atctcgttac gtctcccaaa cggccacacc
     2041 ggccggttcg atcgtgcggg tgccgatgag gaatttcgcg tccttcgggg ccggcgcggg
     2101 cgtcgcgatg tcccggtagt tcagcgcgat cgtgtacggc ccgcgacgga gcacacgcag
     2161 gcgctgtggg agcggcgcag cgcctatgcg cgcgtcgccg acgacctttt cgacaagcgc
     2221 gtcggccagc gatgcatcgc catatacgcc gcaataggtg acggcgcctt cgccatgctt
     2281 ccgccgcgtg atggcggcgg tgccggtgta gaactggtcc gtgtacttcg ccagcacctc
     2341 ggtctctttt tccggcgcga gcaggtccgc ccagacgttc cactgaaacg tcttgtgcgc
     2401 gaaggcgacg gtgccgtggc tgtcatccgg cagcccgtcg taaccggtga tggtcgcgcc
     2461 catgagcggc acgatcggtt tggccagcgg gccttcccac agttggccgt tgcggtccat
     2521 cagtccagag cggcaggtga ggacgagctg accaccacca tcggcaaagt catcgaattt
     2581 gcgg
//
LOCUS       DATIMH010000889         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2523117, whole genome shotgun sequence.
ACCESSION   DATIMH010000889 DATIMH010000000
VERSION     DATIMH010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2523117"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VLI90_15435"
     CDS             <1..697
                     /locus_tag="VLI90_15435"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV15653.1"
                     /translation="EPLGVIGTVVIPVLVFVVLGRMFGRSGGGGPRDAPALVTIDLPV
                     LAAILIALSAVLSLITIVAIYREGGILKRLRATPLRSHTILTAHVIVKLLFTAITLAA
                     TMLAGRRFYAGGIHASFVSFVLALLFSTVSIVSIGFLVASVVPTARFAQPVGTLILYP
                     MLGLSGLFVPLASLPPAARGIARVLPLTYVVSLLRGVWRGDGWLAHAGDITALAVIFL
                     ICTAVSARVFRWE"
     gene            778..1833
                     /locus_tag="VLI90_15440"
     CDS             778..1833
                     /locus_tag="VLI90_15440"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HSV15654.1"
                     /translation="MAVGVLLFAGFAQRVAPPPASAVATKVVGAANTFLATLSEAERT
                     KVTFDFSSNQRTVWSNLPSGIYQRNGLRIGDLSATQREAALALVAAALSRSGYQKVTD
                     IMNGDEVLRNQGGGRTGGRPGAPGAPAAGGGRGGRGGGIQFGSDEYYISFVGAPSATN
                     PWMLQFGGHHLAINVTMVGSDNVMTPSLPAAQPARYVLNGQTIRPLGKENDKGFALIN
                     ALDDNQKKQAILAYQVRDLVLGPGNDGKVIQPEGMLASAMNANQQAMLLDIVHEWVGI
                     LNDEGAAAKMAEVKSNLAKTYFAWSGSTTNGELAYYRIQGPTLVIEYAPQQGDLDHIH
                     TIYRDPTNDYGARLVKH"
     gene            1888..>2111
                     /locus_tag="VLI90_15445"
     CDS             1888..>2111
                     /locus_tag="VLI90_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15655.1"
                     /translation="MKHTWLLAIAIVMLTPPGVGAHRLDEYLQATRVGIERERVSLEM
                     DLTPGVSVARQVTAWIDSDGNRVISTPESLA"
BASE COUNT          366 a          655 c          670 g          420 t
ORIGIN      
        1 cgaaccgctc ggcgtcatcg gcacggtcgt cattcccgtt ctcgtctttg tcgtgctggg
       61 acggatgttt ggtcgatcgg gcggcggggg gccacgcgac gcgccggctc tcgtcaccat
      121 cgacctgccg gtacttgcgg cgatcctgat cgcgctgagc gccgtgctgt cgctcatcac
      181 catcgtggcg atttatcggg agggaggcat cctgaagcga ttacgcgcaa cgccgctccg
      241 ctcgcacacg atcctcaccg ctcatgtgat cgtaaagctg ctgttcacgg cgattacgct
      301 ggccgcgacg atgctggccg gacgtcgctt ttacgccggc ggcattcacg cgtcgttcgt
      361 gtcttttgtc ctggcgctgc tcttcagcac ggtcagcatc gtgtcgatcg ggttcctcgt
      421 cgccagcgtc gttccaaccg cgcgcttcgc gcagcccgtc ggcacgttga tcctgtaccc
      481 aatgcttggt ctctcgggtc tgtttgtccc actcgcgtcg ttgccaccgg cagcgcgggg
      541 aatcgcacga gtccttccct tgacctacgt cgtttcgctc ctgcgcggtg tctggcgagg
      601 cgatggatgg ctggcccatg ccggcgatat cacggcattg gccgtgatct tcctgatctg
      661 caccgccgtg tccgcgcgtg tgttccggtg ggaatgagac gtcacgcttt ttcatttccg
      721 ttccactcgg accgggacta ccttagcacc gtgaaattac tccgcccatt gctcctcgtg
      781 gccgtcggtg tcctcctctt tgctggtttt gctcagcgag tcgcgcctcc gccggcgagc
      841 gcggtcgcaa cgaaagtcgt cggggctgcc aacacattcc tcgccactct cagcgaagcc
      901 gaacgcacga aggtgacgtt cgacttcagc agcaaccagc gcaccgtctg gtccaacctc
      961 ccgtccggca tctaccaacg gaacggactg cggataggag atctcagcgc gacgcagcgc
     1021 gaagcggcgc tcgcactggt ggcggcggct ctcagccgat ccggttacca gaaggtcacc
     1081 gacatcatga atggtgacga ggtcctgaga aaccagggcg gggggcgaac tggcggacgc
     1141 ccgggcgcgc ccggcgctcc cgccgcgggc ggcgggcgtg gcggacgcgg aggaggtatc
     1201 cagtttggca gcgacgagta ctacatctcc ttcgtcggcg caccatcggc gaccaatccg
     1261 tggatgctgc agtttggcgg tcatcatctc gcgatcaacg tcaccatggt cgggtccgac
     1321 aacgtgatga cgccgagcct gcccgccgcg cagccggcgc gctatgtgct gaacggtcaa
     1381 acgattcgcc cgctcgggaa ggaaaacgac aaagggttcg cgctgataaa cgcgctcgac
     1441 gacaaccaga aaaagcaggc gatccttgcc taccaggttc gggatctggt gcttggtcct
     1501 gggaacgacg gaaaggtgat tcaacctgaa ggaatgctcg cgtcggcgat gaacgccaat
     1561 cagcaggcca tgctgctaga catcgtccat gaatgggtgg gtattttgaa cgacgagggt
     1621 gcggccgcga agatggcgga agtcaaatca aacctcgcga agacgtactt cgcgtggagt
     1681 ggttcgacga ccaacggcga gctggcgtat taccggatcc aggggccgac gctggtcatc
     1741 gagtacgcgc cgcagcaggg ggacctggat cacattcaca ccatctatcg ggatccgacc
     1801 aacgattacg gcgcacggtt ggtgaaacac tgactcgcgg ttcgcggttc gctattcgct
     1861 attcgctatt cgcgggcgct gttcgctatg aaacatactt ggctgcttgc gatcgccatc
     1921 gtcatgctga ccccaccggg ggtaggtgcg catcggcttg acgagtatct gcaggcgacg
     1981 cgggtcggca tcgagcgtga gcgcgtgagc ctcgagatgg atctcacgcc gggcgtcagc
     2041 gtggcgcgac aggtgacggc gtggatcgat agcgacggca accgagtgat ctcaacgccg
     2101 gaatcgctgg c
//
LOCUS       DATIMH010000890         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3963231, whole genome shotgun sequence.
ACCESSION   DATIMH010000890 DATIMH010000000
VERSION     DATIMH010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3963231"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            551..>3073
                     /locus_tag="VLI90_15450"
     CDS             551..>3073
                     /locus_tag="VLI90_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HSV15656.1"
                     /translation="MSTPTNTKSAEFESMTSSDVSSTASATAIPATQPALSETLPIDQ
                     FIWGAGIECSFIPHMKVDQFEWTQHDRQWREDFRRAKEEAGITNLRYALPWHVLEREQ
                     GKFDWSYSDERMAEFENLGINLLLDVMHFGTPTWLRQSVGDPEFPEALERFTEELVSR
                     YRKQVKIWCPFNEPLVSALFSGDLGFWPPYQRKWRGYMPVLSRVVQAVSRGIRAIRRA
                     QPEATVLLCDASENYKTRIESLQPEVRRRNMRRFIVMDLLTGRVDRDHPLYTWITSYG
                     LSELDLQWFRSNPQSPDILGLDYYPHSDWQLDMQGGAIKQRRADNPVGLYGVSNAYYN
                     RYGIPLMLTETSIDGKPINREIWLDRMLDEIKQLREEGVPMLGLIWWPLLDQLDWDGA
                     MTHRIGKIHEVGMFNLQRQPDGTLKRISTPLIRLFKQAMAAGEERIGKLEQVVRPAVG
                     VDDKQAPLSTSESWDELEGTSPAIAKKNGNGANGHGTNGNGKSGAVEAALNRLTEPAP
                     GAASEVTAGQTRTATQSPPRMAIDDSKSTDKYGIVVFSHLRWGFVWQRPQQFLSRFAK
                     KHKILFVEEPFFDLKEGSEPRIDYHKVMPNVTVVTPHVSKEWNRSPKMPAKLREFTRT
                     AIDAMNESGEFDRPLLWYYSPMDSAWSLGYFENRGVVYDCMDELNQFTGAPRSLVANE
                     ARLIEHADIVFTGGYELGEKKRKQHDNVHTFGCGVEFDHFGKAADPQTVIPPDIDFMA
                     RPILGWMGVVDERVDYAMVGEMARMRPDWSFAMVGPVVKVDPNLLPHAPNLYWLGGRD
                     YQQLPNYCRAFDVNMMCFANNAATQYINPTKGLEYMATGKPIV"
BASE COUNT          627 a          958 c          969 g          519 t
ORIGIN      
        1 gttcgccaga gaagaccatt tagaaggtcg ccagaccgta cgcgaacgag ccgtcgcgct
       61 gtgccgaacg gcgttaattt acaaagtgag tttgtcatga aacgccccgc cagccaccaa
      121 cgcagcgcta agtcgagtgg accggatccg atttgcctca ccgcatcgtc cgtctggtcc
      181 cccgcctcta aagcgccgcg ttcggcttcg ccatcgtcgt cttcgtcgcc cgcgccttct
      241 caggtctggt cgcgatacat cgacagccgc ctcaagcagt ttgaggccag ggaacgttag
      301 cgcgcgccac agcggcatcg cagcacacag cagttcgtcg cgcactgggt tatgcagcag
      361 ctgatgtggc acgcgcgcca acggctgacg tgccgcggcc tcgcgtggca ctcgcgtgct
      421 agcggatcgc tcactcggtt cagggcagcc gaccggttcg tcgcccgaca ctggattgcg
      481 ttagttgtag atctggttcc ggttcgtaca agccagttct gtgcttggga aggaagagtg
      541 tacgcgcatg atgagtaccc ccactaacac caaatccgcg gagttcgaaa gcatgaccag
      601 cagtgatgtt tcctcgaccg cttctgcaac agccatcccc gcgacgcaac ctgcgttgag
      661 cgagacgctg ccgatcgacc agttcatctg gggcgccggc atcgaatgct cgttcatccc
      721 gcacatgaag gtcgatcagt tcgaatggac ccagcacgac cgccagtggc gcgaggactt
      781 tcgccgcgcg aaggaagagg cgggcatcac caacttgcgc tacgcgttgc cctggcacgt
      841 gctggagcgc gagcagggca agttcgactg gagctacagc gacgagcgaa tggccgagtt
      901 cgagaacctc ggcatcaacc tgctgctgga cgtgatgcac ttcggcacgc caacctggct
      961 tcgccagtcg gttggtgatc cggaatttcc cgaagcgctc gaacgattca ccgaagagct
     1021 ggtctcgcgc tatcgcaagc aggtgaagat ctggtgcccg ttcaacgaac cgctggtcag
     1081 cgcgctgttc agcggtgacc tgggtttctg gccgccgtac cagcgcaagt ggcgcggcta
     1141 catgccggtg ctgagccgcg tggtgcaggc agtcagccgc gggatccgcg cgatccgccg
     1201 cgcgcaaccg gaagccacgg tgctgctgtg cgacgccagc gaaaattaca agacgcgcat
     1261 cgaaagtctg caaccggaag ttcgccggcg aaacatgcgg cgattcatcg tgatggacct
     1321 gctgaccggc cgcgtcgatc gcgatcatcc gctgtacacg tggatcacca gctacggcct
     1381 gagcgagctt gatctgcagt ggttccgcag caacccgcag tcgccggaca tcctcgggct
     1441 cgattattac ccgcacagcg attggcaact ggacatgcag ggcggcgcga tcaagcagcg
     1501 ccgcgccgac aatccggtcg gcctctacgg cgtcagcaac gcgtactaca accgctacgg
     1561 catcccgctg atgctgacag agaccagcat cgacggcaaa ccaatcaacc gcgagatctg
     1621 gctcgaccgg atgctcgacg aaatcaaaca gctgcgcgaa gagggcgtcc caatgctcgg
     1681 cctgatctgg tggccgctgc tcgaccagct ggactgggac ggcgcgatga cccatcgcat
     1741 cggcaagatt cacgaagtcg gaatgttcaa cctgcagcgc cagccggacg gcacgctgaa
     1801 gcgaatcagt acgccgctga tccgattgtt caagcaagcg atggccgccg gtgaagagcg
     1861 gatcgggaag ctcgaacagg tcgtccggcc cgccgtcggc gtggacgaca agcaggcacc
     1921 gctttcgacc agcgaatctt gggatgagct ggaaggcacg agcccggcga tcgccaagaa
     1981 gaacggcaac ggcgcgaacg gtcacggcac caacggcaac ggcaagagcg gcgcggtgga
     2041 agcggcgctc aatcgcctga ctgagcccgc gcccggcgcc gccagtgaag tgacggccgg
     2101 ccagacccgc accgcgacgc agtcgccgcc gcgcatggcg atcgatgaca gcaagagcac
     2161 cgacaagtac ggcatcgtcg tcttcagcca cctgcgctgg ggtttcgtct ggcagcgccc
     2221 gcagcagttc ctctcgcgct tcgccaagaa gcacaagatc ctcttcgttg aagaaccctt
     2281 cttcgacctg aaggaaggga gcgagccgcg gatcgattat cacaaggtga tgcccaacgt
     2341 cacggtggtg acgccgcacg tgtcgaagga gtggaaccgc agccccaaga tgcccgccaa
     2401 gctgcgcgag ttcacccgca ccgcaatcga cgccatgaac gagagcggtg aattcgatcg
     2461 cccgctgctg tggtattact cgccgatgga ttcagcctgg tcgctgggct atttcgagaa
     2521 tcgcggagtg gtgtacgact gcatggacga actgaaccag ttcaccggcg cgcccaggtc
     2581 gctggtcgcc aatgaagcgc ggctgatcga acatgcggac atcgtcttca ccggcggata
     2641 tgagctgggc gagaagaagc gcaagcagca cgacaacgtg cataccttcg ggtgcggggt
     2701 ggagttcgac cacttcggca aggcggcgga tccgcagacg gtgatcccgc cggacatcga
     2761 tttcatggcg cggccgatcc tgggttggat gggggtggtg gacgagcgtg tggattacgc
     2821 gatggtgggc gagatggcgc ggatgcggcc ggactggtcg ttcgcgatgg tcggcccggt
     2881 ggtgaaggtg gatccgaacc tgctgccgca cgcgcccaac ctgtattggc tgggcgggcg
     2941 cgattaccag cagcttccca attattgccg cgcattcgac gtcaacatga tgtgcttcgc
     3001 caacaacgcc gccactcagt acatcaaccc gaccaagggc ctggagtaca tggcgaccgg
     3061 caagccgatc gtc
//
LOCUS       DATIMH010000891         8070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5703326, whole genome shotgun sequence.
ACCESSION   DATIMH010000891 DATIMH010000000
VERSION     DATIMH010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5703326"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VLI90_15455"
     CDS             <1..903
                     /locus_tag="VLI90_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15657.1"
                     /translation="VIVRHTFVEALVQPIYSLLLALGAVILLIFAMLPFFTLGEDTTM
                     FKAVGLDIVLLLVLISTLFATSKSIFEEIEDRTMLTLMSKPVRKWEVLVGKYFGIVLS
                     ALLAVVILGTLLILCTYWRTPGDYMLRTTTLDDRELQQIWDYRRMHMSGLFPSLLLVW
                     LQISVLAAIGVALSTRFSLVVNLPVVILLYIAGNLTRFLFPMTAGSGSVMSDRPAIVK
                     GIVYVVSLVLPYLQTFDLRPLTVYKPIALVGSPFAYDPQSVTLGTIWACVGVAVLYAI
                     AYSAFALSAGMWLFEGRELGGAEG"
     gene            complement(988..2097)
                     /locus_tag="VLI90_15460"
     CDS             complement(988..2097)
                     /locus_tag="VLI90_15460"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV15658.1"
                     /translation="MSQEQQQHKSTRVGIIGGGWPGVAHARGYRAAGGFQIVAVADLI
                     PERRQQLVVESGGKARAYADAKELIADKEIDGVSVCLPTDLHVQIARAAMRAGKHVVV
                     ETPPGLSVREAKQLAAASAKYGKLLAYAFQRRFGVAELAARQAIEKGYAGEIYHARAT
                     WMRTRGVPIGTGWYVHRAKSGGGALIDIGSHMLDTAWNLLGQPRPVSVYAVTHRQLTE
                     HDVEDAAFALVRFDGGQTLELGTSWAINQAPSQNGVTCRVYGKAGGIEVYTPQGAVLY
                     RQFNAKGEAKASALKPPKVVGHAAMMRHFRECLLGKAQPAMGAVQGVVLMQMIEALYK
                     SEETGRSVEIKAAAVESPDSIDAESAPALAVTVTS"
     gene            2531..3310
                     /locus_tag="VLI90_15465"
     CDS             2531..3310
                     /locus_tag="VLI90_15465"
                     /inference="COORDINATES: protein motif:HMM:NF018936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 family protein"
                     /protein_id="HSV15659.1"
                     /translation="MSELSREQFVAELLEHVKVKFPLVKIAEGEQPFSLRVNGQLAGL
                     ENIYRMVILRPQERLHHIDRWIVELLRAAEGTPDRTGKFDDVKDRILPMVLPGVAAES
                     YKGTVTSSLVDGLVVAYAIDQDRTISYIPRERFEHWNISEEDLHELAINNLVTRSETL
                     AAHAAEDDDGEVNLILFQTMDGYDASRVLLPTLHERLRPYLGSPFGAAIPNRDILLCF
                     RNDAETVARLKAQVVEDYRQMPHQVTDQLLLITPDGIAVRE"
     gene            3459..4238
                     /locus_tag="VLI90_15470"
     CDS             3459..4238
                     /locus_tag="VLI90_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSV15660.1"
                     /translation="MKFGICTSITNAAAVKAAGWDYVEENVQSLLQGTVTDEAWTNES
                     AANAAVLPVPAANSLVPASLKITGPDVDPNALGSYMETVIRRAATVGIQTLVFGSGAA
                     RNVPDGFDRERAVEQITDFVRSSADLAADADIMLVAEPLNRGECNIINTIDEAMQYVR
                     EVDRPNFQCLLDTYHLWLEDEPLVNLQAAMPWIKHVHLADKDGRVAPGLSGTADYRPV
                     FKILKGAGYDGLISFEGHVMPDFAVTAPKVLSFIKRQWSEA"
     gene            4382..5461
                     /gene="serC"
                     /locus_tag="VLI90_15475"
     CDS             4382..5461
                     /gene="serC"
                     /locus_tag="VLI90_15475"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HSV15661.1"
                     /translation="MSHRIFNFSAGPAMLPTEALEKSAAALLDYQGKGFGIAEVSHRG
                     KEFDGVVDEAVTRCKQLLGLGDTHDVLFLQGGATQLFTLIPMNFLHGSADYLVGGEWS
                     KKAASFGKEIGKINVVASSEASNFDHSPPPSDWKLNADADYFHVCTNETVHGHRLPAW
                     PTHKNLIVDASSEFMSRPHPVQDCALVYGGAQKNLGPSGVVLAIVRKDMYAKQKKVPS
                     KLWSFKDQAESKSMINTPPTFGIYILLEVFRWLEAQGGLAAMEKRNAIKANLIYDTID
                     NSNGFYKGTVSVKEQRSHMNVTYRLPSDELTDEFVKTAAKQGMYGLKGYRTVGGIRAS
                     IYNAMPVEGCQALANFMKDFASKKS"
     gene            complement(5493..5723)
                     /locus_tag="VLI90_15480"
     CDS             complement(5493..5723)
                     /locus_tag="VLI90_15480"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HSV15662.1"
                     /translation="MSVRLPSFKPRDLIALLKRAGFEQVYQDSSHLYLRHPRTSRATC
                     VPIHSGDVKRNLARKILLNDCALTISEVQKLL"
     gene            complement(5720..6064)
                     /locus_tag="VLI90_15485"
     CDS             complement(5720..6064)
                     /locus_tag="VLI90_15485"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSV15663.1"
                     /translation="MTAQERTALELAARKHGFRGVADYLRAVAFSGKAPAEYTYTVRI
                     HPGDADESGFWAEVPALSGCNAQGETYEQTIEHARQAIEGYLRMLIKLGEPIPIEKQP
                     RAVTVTAVKVAV"
     gene            complement(6133..7962)
                     /locus_tag="VLI90_15490"
     CDS             complement(6133..7962)
                     /locus_tag="VLI90_15490"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSV15664.1"
                     /translation="MTRRHCLLWILTAFVLACLNSAALARTSADVSAALSTTAVHAGD
                     SATIAIAITVHPGLHAQSHTPLEKNLIPLVVKLDVNPAADFKDPLYPPPQVENFPQLG
                     TVSAYTGRVIVQVPVTVKPDAPLGALNITGSVRYQACNDTACFPPETQKFEVDTQVVA
                     SGVKTEANQAELFRELALPTSAPTTQAAAVVPQSSGGTPPPPPSRIFGRELGKDAYLL
                     AFAGAFVVGIIFNAMPCVLPVVPLKVMGFYEVSRHDRRKSVALGAVFSAGLIASFGVL
                     ALLVVALRVLDWGGLFQKTWFTLAIVTVLVVMAITLFGFFTVNLPNRVYRFTPRHDTY
                     VGNFLFGILTAALSTPCTFGMFVGLLAWALAQPASIGVALIMMVGVGMAFPYLLLSAF
                     PEVARRFPRAGPWAEVVKQMMAFLLLATAVYFAQPLYERFVSDQAFWWTLFAVIAAGG
                     LFLIVRAFQLSERMTPRLIAVAIAIALVGPSFWATRRLTIRPFEWQPYSDHALADARA
                     AGKPVLIDFTASWCGNCHWVEAYVLHDPKIVRAVHDHNLVMIKADVTDETAPARPLLG
                     KLNPAGAIPLTAIYAPKSDDPRLLDGIYSVDDLLSAIAKTTGG"
BASE COUNT         1542 a         2598 c         2467 g         1463 t
ORIGIN      
        1 gtcatcgtcc gccacacgtt cgtcgaagcg ctcgtgcagc cgatctattc gctgctgctg
       61 gcgctgggcg cggtcatcct gctgattttc gcgatgctgc cgttcttcac acttggtgaa
      121 gacacgacga tgttcaaagc cgttgggctc gacatcgtcc tgctgctggt gctcatttcc
      181 acgctgtttg ccaccagcaa atcgatcttt gaagaaatcg aagaccgcac gatgctcacg
      241 ctgatgagca aaccggtgcg caagtgggaa gtgctggtcg gcaagtactt cggcattgtg
      301 ctgtcggcgc tgctggccgt ggtcatcctc ggcacgctgc tgatcctgtg cacctattgg
      361 cgaacgcccg gcgattacat gctgcgcact accacgctgg acgaccgcga gctgcagcag
      421 atctgggact atcgccggat gcacatgagc ggcctgttcc ccagcctgct gctggtctgg
      481 ttgcagatta gcgtgctggc cgccatcggc gtggcgctgt ccacgcgttt ttcgctggtg
      541 gtcaacttgc ccgtcgtcat cctgctttac atcgccggca acctcacgcg gttcctgttc
      601 cccatgaccg cgggctccgg ttcggtcatg tccgaccgcc cggcgatcgt gaaggggatc
      661 gtttacgtcg tgagcctggt gctgccatac ctgcagacgt tcgacctgcg cccccttacg
      721 gtctacaagc cgatcgcgct ggtcggctct ccctttgcgt acgatcccca atccgtcacg
      781 ctcggcacca tctgggcctg cgtcggcgtc gcggtgctgt acgccatcgc ttacagcgcc
      841 ttcgccctta gcgccggcat gtggctcttc gaaggccgcg agctcggtgg cgctgaagga
      901 taaactcgtc cgccgttctc gaatcgtttc gtcgccgctt gcggcttcgc aaaccacacc
      961 gcccggcctg ctaagccgca accggcgtca agacgtcacc gtcaccgcca acgccggcgc
     1021 agattcagca tcgatcgaat ccggcgactc gactgctgcc gctttgatct ccacgcttct
     1081 tcccgtctcc tcgctcttgt acagcgcctc gatcatctgc atcagcacca cgccctgcac
     1141 cgcccccatc gcgggctgcg ctttgcccag cagacattcg cgaaaatgcc gcatcatcgc
     1201 cgcgtggccc acaaccttcg gcggtttcag cgccgacgcc ttcgcctcgc ctttggcgtt
     1261 gaactggcga tacagcaccg cgccctgcgg cgtgtacacc tcgatcccgc ccgccttccc
     1321 atacacccgg cacgtcacgc cgttctgcga cggcgcctgg ttgatcgccc aactcgtccc
     1381 caactccagc gtctgcccgc cgtcgaatcg caccagcgcg aacgccgcgt cttcgacatc
     1441 gtgctcggtc aactgccgat gcgtgaccgc gtacaccgac accggccgcg gctggccgag
     1501 caggttccac gcggtatcga gcatgtgtga gccgatgtcg atcagcgcgc cgccgccgga
     1561 cttggcgcga tggacatacc atccggtgcc gatcggcacg ccgcgcgtgc gcatccacgt
     1621 cgcccgggcg tggtagattt caccggcgta tcccttctcg atcgcctgcc gcgccgccag
     1681 ctccgcgacg ccaaagcgac gctgaaacgc gtacgccaac aacttgccat acttggcgga
     1741 cgccgccgcc agttgcttcg cctcgcgcac gctcaaaccc ggcggcgttt cgacgaccac
     1801 gtgcttcccg gcgcgcatcg ccgcccgcgc gatctggacg tgcaaatccg tcggcagaca
     1861 aacgctgacc ccgtcgatct ccttgtcggc gatcaactcc ttcgcgtccg cgtacgccct
     1921 cgccttgccg ccggattcga ccaccagctg ctgtcgccgc tccggaatca aatccgccac
     1981 cgcgacgatt tgaaaacccc cggccgcgcg atagccgcgc gcgtgcgcca cacccggcca
     2041 cccgccacca atgatcccga cgcgcgtgct tttgtgctgc tgctgttcct gagacatctc
     2101 gctcgctcct ctggtcgtag agagccgcga gttataccgg tcgatgcggg aaggtggaat
     2161 gtgcatgaac gcgcgggggc cggcggagca cgaatggcgc ggagagcgac catctgacca
     2221 tggcggctgt gagggctgga actctccgcg caactcaaat tatgcaaacg ttcagagaaa
     2281 tgcagtaaag atcctgcggc atcaggaatc tcctgtagca gcgacgcctg cgtcgcctta
     2341 tttccaattc cagcgaagag ggcgatacag gcgttctggg cagtcgcgtc cggctctttc
     2401 ccctctcccg gcgtaccggg agaggggacc ggagcgcgca ccagctatct gcgtttgccc
     2461 cgaggcaggc atcgccccaa catcctttcg catggttcgc ggtcggcgca gaagctatac
     2521 tgtctggtag atgagcgaac tttcgcgcga gcagtttgtt gcggagcttt tagagcacgt
     2581 caaagtgaag ttccccctcg ttaaaattgc tgaaggcgag cagccgtttt cgctgcgcgt
     2641 gaacgggcag ctcgcggggc tggagaacat ttatcgcatg gtgatcctgc ggccgcagga
     2701 gcggctgcat cacatcgacc ggtggatcgt cgagctgctc cgcgctgccg agggcacgcc
     2761 cgatcgcacg gggaagttcg acgatgtgaa agaccgcatc ctgccgatgg tgctgcccgg
     2821 tgtggccgcg gaatcgtaca aggggacagt gacgtcttcg ctggtcgatg gcctggtcgt
     2881 cgcgtacgcg atcgaccagg accgcacgat cagctacatc ccgcgcgagc gcttcgagca
     2941 ctggaacatc tccgaggaag acctgcacga gttggcgatc aacaacctcg tcacccgcag
     3001 cgaaacgctg gccgcccatg cggccgagga tgacgacggg gaagtgaacc tcatcctgtt
     3061 ccagacgatg gacggctacg acgccagccg ggtgctgctg cccacgctgc acgagcgcct
     3121 gcggccgtac ctcggcagcc cgttcggcgc ggcgattccc aatcgcgaca tcctcctgtg
     3181 cttccgcaat gacgcggaaa cggtcgcgcg attgaaagcg caggtggtgg aagactatcg
     3241 ccagatgccg caccaggtga cggaccagct gctattgatc acgccggatg ggattgcggt
     3301 tcgcgaataa aaacgcactg ttcgcgcgca cccccgccgc ttgcggcttc gcagaccgcc
     3361 ccgcgtggtt tgcgaagccg caagcggctg aagcgagtct ctagcatcgc cccgttcctt
     3421 cccctattat ccccgactca attgaaggag accggttcgt gaagttcggc atttgcacgt
     3481 caatcaccaa cgccgccgcc gtgaaggcgg cggggtggga ctacgtggaa gagaacgtgc
     3541 aaagcctgct gcagggaacg gttacggacg aggcttggac gaacgaatcg gctgcgaacg
     3601 ccgcggtgtt gcccgtgccc gcggcgaact cgctggtgcc ggcgagcctg aaaatcaccg
     3661 gacccgatgt cgatccgaac gcgctcggca gctacatgga aaccgtcatc cgccgggcgg
     3721 cgacggttgg catccagacg ctcgtcttcg gcagcggcgc cgcccgcaac gtgcccgatg
     3781 gatttgatcg cgagcgcgcg gtcgagcaga tcaccgactt cgtccgcagc agcgccgatc
     3841 tggccgccga cgccgacatc atgctcgtcg ccgagccgct caaccgcggc gaatgcaaca
     3901 tcatcaacac gatcgatgaa gcgatgcagt atgtgcgcga ggtcgatcgc ccgaatttcc
     3961 aatgcctgct cgacacgtat cacctgtggc tcgaagacga accgctggtg aacctgcagg
     4021 cggcgatgcc atggatcaag cacgtgcacc tggcggacaa ggatgggcgg gtggccccgg
     4081 gcctgagcgg caccgcggat tatcgcccgg tgttcaagat cctgaagggc gccggttacg
     4141 atggcctaat cagttttgaa ggccacgtga tgccggactt cgccgtcaca gcgccgaagg
     4201 ttctgtcgtt catcaaacgc caatggagcg aagcataaag ttcaaccgcg acacgagcct
     4261 ccggcccgtg cgtgaatcgc ggaagtagaa taagttctgt aatcgaagac tttattgaat
     4321 tgagccatcc gcacgggcca gaggcccgtg tcacggtggc gcgtttttag gaatcaaaac
     4381 gatgtcacac cgcattttta atttcagcgc cggccccgcg atgttaccca ccgaggccct
     4441 ggaaaaatcc gccgccgcgt tgctcgacta ccagggcaaa ggcttcggca tcgccgaagt
     4501 cagccaccgc ggcaaagagt ttgacggcgt cgtcgatgaa gcggtcacgc gctgcaaaca
     4561 gctgcttggc ctgggcgaca cgcacgatgt gctgttcctg caaggcgggg cgacgcaatt
     4621 gttcacgctc atcccgatga acttcctgca cggcagcgcc gactatctcg tcggcggcga
     4681 gtggagcaag aaggccgcca gcttcggcaa ggagatcggc aagatcaacg tcgtcgccag
     4741 cagcgaggcg agcaacttcg atcattcccc gccgcccagt gattggaagc tgaacgccga
     4801 cgcggattac ttccacgtct gtacgaacga aaccgtgcac ggccatcgcc tgccggcctg
     4861 gccgacgcat aagaacctca tcgtggacgc cagcagcgaa tttatgtcgc gcccgcatcc
     4921 ggtgcaggat tgcgcccttg tctacggcgg cgctcaaaag aacctcggcc ccagcggcgt
     4981 cgtcctcgcc atcgttcgca aggacatgta cgcgaagcag aagaaggtgc cgagcaagct
     5041 gtggagcttc aaggatcagg ccgagagcaa gagcatgatc aacacgccgc cgacgttcgg
     5101 catttacatc ctgctcgaag tcttccgctg gctggaagcc cagggcgggc tggccgccat
     5161 ggaaaagcgc aatgctatta aagccaacct gatttacgac acgatcgaca acagcaacgg
     5221 cttctacaag ggcaccgtca gcgtgaaaga gcagcgttcg cacatgaatg tcacgtaccg
     5281 cctgcccagc gacgagctga cggacgagtt cgtgaagacc gccgccaagc agggcatgta
     5341 cggcctcaaa ggctaccgca ccgtgggcgg catccgcgcc agcatctaca acgcgatgcc
     5401 ggttgaagga tgccaggcgc tggcgaactt catgaaggac tttgcgtcga agaagtcgtg
     5461 aatccaccgc gcgggtcgcg cgatcctcgc cgtcacagca gtttctgaac ctcgctgatg
     5521 gttaaggcgc aatcgttgag caggatcttt ctcgcgagat tgcgtttcac gtcgccgctg
     5581 tgaatcggga cgcacgtcgc gcggctggtt cgcggatgac gcaggtataa gtgtgatgag
     5641 tcctggtaca cctgttcgaa gccggcgcgc ttcaacaggg cgatcaaatc gcgcggcttg
     5701 aacgaaggca ggcgaacgct cataccgcga ctttcaccgc tgtgacggtg acggcgcgtg
     5761 gttgcttttc aatggggatc ggctcaccaa gcttgatgag catgcgcagg tatccctcga
     5821 tcgcctgccg ggcgtgttcg atggtctgct catatgtctc tccttgcgcg ttgcatcccg
     5881 aaagggcagg cacctcggcc cagaagccgc tttcgtccgc atcgcctgga tgaattcgaa
     5941 cggtgtatgt gtattccgcc ggcgctttgc cggaaaacgc taccgcgcgc aaatagtccg
     6001 ccacaccgcg aaacccatgc ttgcgggcag ccagttccaa agccgtgcgt tcctgggcgg
     6061 tcacgcgaac gttcagctgc tgcgttcgtc gcttcatgct agcaagtata gcacaaagtc
     6121 acgaccagga catcatcccc cggtggtctt cgcgatcgcg ctgagcagat catcgacgct
     6181 gtagatccca tcaagcagcc gcgggtcgtc agacttgggc gcgtagatcg ccgttagcgg
     6241 gatcgccccg gcggggttca gcttgcccag cagcgggcgt gctggcgcgg tttcgtccgt
     6301 cacgtcggcc ttgatcatca ccagattgtg gtcgtgtacc gcgcgcacga tcttcgggtc
     6361 gtgcagcacg tacgcttcga cccaatggca gttgccgcac cagctggcgg tgaaatcgat
     6421 caacaccggc ttgcccgcgg cgcgggcgtc ggccagtgcg tgatcggaat agggttgcca
     6481 ttcgaacggc ctgatcgtca accggcgggt ggcccagaag gacgggccga ccagcgcgat
     6541 ggcgattgcg acagcgatga gccgcggtgt cattcgttcg gacagctgaa acgcgcgcac
     6601 gatcaggaag agcccaccag cagcgatgac ggcgaacagc gtccaccaga acgcctgatc
     6661 ggaaacgaat cgttcataaa gcggctgtgc gaaatagacc gcggttgcca gcagcaggaa
     6721 cgccatcatt tgcttgacga cttccgccca cgggccggcc cgggggaatc gccgtgcaac
     6781 ctcggggaag gcgctgagca gcaggtacgg gaaggccatg ccgacgccga ccatcataat
     6841 gagggccacg ccgatactcg ccggctgcgc cagcgcccac gccagcaggc cgacgaacat
     6901 cccaaatgtg cacggtgtcg acagtgccgc cgtcaggatg ccgaaaagga agttgcccac
     6961 gtacgtgtca tgccgcggcg tgaaccggta aacgcgattg ggcagattaa cggtgaagaa
     7021 accgaacagc gtgatcgcca tcacgaccag cacggtgacg atcgcgagcg tgaaccacgt
     7081 cttctggaac agtccacccc agtcgagcac gcgcagcgcg acgacgagca gcgccagcac
     7141 gccgaagctg gcgatcaggc ccgcgctgaa gaccgctcca agcgccacgc tcttgcggcg
     7201 gtcgtgccgc gagacttcgt agaaccccat aaccttcagc ggcaccaccg gcagcacgca
     7261 tggcatcgcg ttgaagatga tgccgacgac gaacgcgccg gcgaaggcga ggagatacgc
     7321 gtctttgccg agctcgcgac cgaagatgcg ggacggcggc gggggaggcg tgccgccgct
     7381 gctttgcggc accaccgcgg cggcttgcgt ggtcggcgcg ctcgtcggta gcgccagttc
     7441 gcgaaacagc tccgcttgat tcgcctccgt cttcacgccc gaggcgacga cctgcgtgtc
     7501 cacctcaaac ttctgcgtct ccggtggaaa gcaggcggtg tcgttgcacg cctgatatcg
     7561 cacgctgccg gtgatgttca gcgcgcccag cggcgcatca ggtttcaccg taaccggcac
     7621 ctgcacgatg actcggccgg tgtacgcgct gacggtgccg agctgcggga agttctcaac
     7681 ctgcggcggc ggatagagcg ggtccttgaa atcggctgcg ggattgacgt caagcttcac
     7741 gaccagcggg atgagattct tctccagcgg cgtgtgcgac tgcgcgtgca ggccggggtg
     7801 tacggtgatg gcgatcgcga tcgttgcgct atcgccggcg tgaaccgcgg tggtgctgag
     7861 ggcggcggag acatcggccg aggtacgcgc caacgccgcg ctgttaaggc atgcaagaac
     7921 gaacgcggtg agaatccaaa gcaggcagtg gcggcgggtc atagacttga tcatagcgtc
     7981 atctgatacg cacgccgggg cttttcagat gattgcacgc ctctcgaatg cagaatgatg
     8041 aatcaaatac aatgcaacaa aagagccgcg
//
LOCUS       DATIMH010000892         4100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2523866, whole genome shotgun sequence.
ACCESSION   DATIMH010000892 DATIMH010000000
VERSION     DATIMH010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2523866"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..789)
                     /locus_tag="VLI90_15495"
     CDS             complement(7..789)
                     /locus_tag="VLI90_15495"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV15665.1"
                     /translation="MGCAPIARSSAARHRRRAFTLVELLVVIGIIALLVAILLPTLAK
                     AREAAARAACLSNLRQVHQSFLFYASVYHDEVPLGFRHDSVPPLGSKQFNSMVYSQTT
                     GQFTLFGWLYNERLMLQPRIFYCPAENDPRQQFDTQPNPWPASALVAPTINVYAGYGC
                     RPEVALPDAPDPVQFAALHIDMPRLAKFKNKAIFADLVSIPTRVDTRHVRGINVLYGN
                     GAAHWVDRKAFDSYLKLCGNPFPPTSANNAYQDSIWAAMDQQ"
     gene            complement(1081..1704)
                     /locus_tag="VLI90_15500"
     CDS             complement(1081..1704)
                     /locus_tag="VLI90_15500"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HSV15666.1"
                     /translation="MINISRAISITAVTAIAAAAAIAIPPSTQPGEGGATARNERPTT
                     DRSGSADQAFIEQATQGGLAEVTLGRLAAQRAVNPQVKQFGQAMVDDHSQANSELLSL
                     AMDRGVRPPVDMQSNDRALLDQLNLAKGADFDRTYINAMVDDHRKDVDAFKKEADNGQ
                     DALVKAWASRMVPTLQHHLQMAEDLQRALNQPLGAAQTAGQKTEPGQ"
     gene            complement(1720..2106)
                     /locus_tag="VLI90_15505"
     CDS             complement(1720..2106)
                     /locus_tag="VLI90_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15667.1"
                     /translation="MARAALESIGLVARHKKFIAIEQRERAQVALRDVPHFALRVGHD
                     KIAFARLRRVVAAEDLVDKFRVKTGFVDWVGMRNLAHRQRDDLDEQPLSDRHGHQNKP
                     DRGQSRTVIFSPLGSRFAGLGSPELT"
     gene            complement(2087..2680)
                     /locus_tag="VLI90_15510"
     CDS             complement(2087..2680)
                     /locus_tag="VLI90_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15668.1"
                     /translation="MMEVTSPLVRKIVDAVPDAYRRLVVHGDAPAVARELLQDVKPAQ
                     LLAQPITSPTAADALLAALWLWHDALDESHRISQRIETPDGSYWHGIMHRREGDFSNS
                     KYWLARCRNHPAGAAIAQQLPAIIAGHSANDPAVARLNANGEWDPFAFVDLAEEVHDR
                     PDDPRHGLTVAIQRLEWRALFEFCARRATATGGASRA"
     gene            2877..>4100
                     /locus_tag="VLI90_15515"
     CDS             2877..>4100
                     /locus_tag="VLI90_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15669.1"
                     /translation="MPINDRLALAAGIGLVLPNRLLLQCGLRRRLDGWRVATGMVLRG
                     EAATFVLSAFSIDIWSDAQPPPTLPDPQQARAHIAERLATFLSGEKEAHVHVRREPRS
                     DRAQRCDGPAYTVRFERWLDGAARFVRPDCWTGDDCTRTCKDNAADVLLNIVCRVRPD
                     GVTADLWLRANHIGIDGVPLQDIITRLQAAWGVVAAVTYPTPEEFAPHASPRACAGRP
                     ELAEIQAFIDFSPLLAWRQRENARLPEAMPIAAAILWRLARHPTFAPLRIGTTVEMPA
                     MADLGRGVGVVVVQPAKYADRRDGLAHFVRDYNDQAKLTKRRRSGGCQTLDAAARLPA
                     RCEIALLRHALRGDAAFGSLGFTMIRDGKIFGAPLGDVGHPHGFIAIGSVALPTPDGR
                     RVGCVTVKGPPQRIAG"
BASE COUNT          718 a         1420 c         1219 g          743 t
ORIGIN      
        1 cgatgatcac tgctgatcca tcgccgccca gatgctgtct tgatacgcgt tgttcgcgct
       61 ggtcggcggg aagggatttc cacacagctt cagatacgaa tcgaacgcct tgcgatcgac
      121 ccagtgggcg gccccgttgc cgtacaggac gttgatcccg cgcacgtggc gcgtgtccac
      181 ccgcgtcggg atggaaacca ggtccgcaaa aatcgctttg ttcttgaact tcgccaaccg
      241 cggcatgtcg atgtgcagcg ccgcgaactg caccgggtcc ggcgcatccg gcagcgcgac
      301 ctccgggcga cagccgtaac ccgcgtacac gttgatcgtc ggcgccacca gcgctgacgc
      361 cggccagggg tttggctgcg tgtcgaactg ctgccgcggg tcgttttcgg cggggcaata
      421 gaaaatccgc ggctgcagca tcaaccgctc gttgtacagc cagccgaaca gcgtgaactg
      481 cccggtcgtc tggctgtaca ccatgctgtt gaattgctta gacccgagcg gcgggaccga
      541 gtcgtgccga aaccccagcg gcacttcatc gtgatagacg ctcgcataga agaggaacga
      601 ctgatgcacc tggcgcaggt tgctcaggca agccgcccga gccgcggctt cgcgcgcctt
      661 ggccaatgtc ggcagcagga ttgctacgag cagcgcgatg atcccaatca ccaccagaag
      721 ttcaacgagc gtgaacgcgc ggcgacgatg acgcgcggcc gatgatctgg cgatgggagc
      781 acaacccatc gcagctttat accaagaccc agttgtgtcc gcagccccgg ttattgtggc
      841 gggcgctcaa gttcgaaagg aaatgcaacc gccgagacgc cgagagcgcc gagttttcac
      901 gccgagaaaa caagaaagag ttggaaccgc agatttcgca gatttcgcag attcagaaaa
      961 gtgtctcgca atctgcgaaa tctgccgtta ataagtcttc tctctttctc tctctcttct
     1021 cggcgtgcgc ctcggcgctc tcggcgcccc ggcggttgca tctgcatttg aaattgaagc
     1081 ctactgtccc ggctccgtct tttgccccgc ggtttgggcc gctcccagcg gctggttcag
     1141 cgcccgctgc aggtcctccg ccatctgcag atgatgctgc aacgtcggca ccatccggct
     1201 ggcccacgcc ttcaccagcg cgtcctggcc gttgtcggct tccttcttga acgcatccac
     1261 gtccttgcgg tgatcgtcca ccatcgcgtt gatgtacgtg cgatcgaaat ccgctccctt
     1321 ggccaggttc agttgatcga gcaacgcgcg atcgttggac tgcatatcga ccggcggccg
     1381 gacgccgcgg tccatcgcca gcgacagcaa ttcggaattc gcctgcgagt ggtcgtccac
     1441 catcgcctgg ccgaattgct tcacctgcgg attgactgcc cgctgcgccg ccaaccggcc
     1501 cagcgtcacc tccgccaacc cgccctgggt ggcttgctcg atgaatgcct ggtcggcgct
     1561 accgctgcgg tcggtggtgg ggcgttcgtt tcgcgcggtt gccccgcctt caccaggctg
     1621 ggtggacggc gggatcgcga tcgccgccgc cgccgcgatc gccgtgacgg cggtaatgct
     1681 gatggctctg ctgatgttga tcatggtcgg ctccttcgtt caggtgagtt caggtgaacc
     1741 aagtcctgca aaccgagagc cgagcggcga aaaaatgacg gttctcgact gcccccgatc
     1801 aggtttattc tgatggccat gccgatcact gagcggttgt tcgtcgagat cgtcccgctg
     1861 acggtgggcc agattccgca tccccaccca atcgacgaag cccgttttga ccctgaactt
     1921 atctacaagg tcctcggcag ctacaacgcg tcggagacga gcgaatgcta ttttgtcatg
     1981 gccaacccgc agcgcgaaat gtggtacatc ccgcagcgcc acctgcgcgc gttcgcgctg
     2041 ctcgatagcg atgaattttt tatgccgcgc gacgaggccg atcgactcaa gcgcggctcg
     2101 cgccaccagt cgccgtcgcg cgccgggcac aaaattcgaa cagcgcccgc cactccagcc
     2161 gctgaatcgc gaccgtcagc ccatgccgcg gatcatccgg gcgatcgtga acctcctcgg
     2221 ccagatcgac aaaagcaaac ggatcccact caccgttcgc gttcaaccgc gccaccgcgg
     2281 ggtcgttcgc cgaatggccg gcgatgatcg ccggcaactg ctgcgcgatc gccgccccag
     2341 ccggatgatt ccggcatcgc gccagccaat atttcgagtt ggaaaaatcc ccctcccgcc
     2401 ggtgcatgat gccgtgccaa tagctgccgt ccggcgtttc gatccgctgc gagatgcgat
     2461 gcgattcatc aagcgcatcg tgccaaagcc aaagcgccgc cagcaacgca tcggcggcgg
     2521 tgggtgaggt gatcggctgc gcgagcaact gcgcgggttt cacatcctga agaagctcgc
     2581 gggcgacagc cggtgcatct ccgtgcacca cgagccgtcg atacgcgtca ggaaccgcgt
     2641 caacgatctt ccgaacgagc ggcgaagtca cttccatcat ggcggcattc tatcaacgtg
     2701 tgcccaccca cgccgggttc ggagcgtact cgcgacgacc cggatgcgtg gaggaaatgc
     2761 agaatgaaga acgcagaatg cagaatggaa gaccgacgat cgcttgtcca ttctgcattc
     2821 tgcattctgc attcgaacga catccgttcc cccatctcga ccgtactggc catcgtatgc
     2881 cgattaacga ccgcctcgcc ctcgccgctg ggatcggcct cgtcctgccc aaccgcctgt
     2941 tgctgcagtg cggcctgcgc cgccggctcg atggctggcg cgtcgccacc ggcatggtcc
     3001 tccgcggcga agccgccacc ttcgtcctct ccgcattttc gatcgacatc tggtccgacg
     3061 cccagccccc gccaactttg cctgatccac aacaggcccg cgctcacatt gccgagcgct
     3121 tagcgacgtt tctcagcggc gaaaaagaag cgcacgttca cgtgcgacgc gagcctcgat
     3181 ccgatcgcgc ccagcgctgc gatggccccg cctacaccgt gcgcttcgag cggtggctcg
     3241 acggcgccgc gcgattcgtc cgccccgact gttggaccgg cgacgattgc acgcggactt
     3301 gcaaagacaa cgcagccgac gtcctgctca acatcgtctg ccgcgtccga cccgacggag
     3361 tgacagcgga cctctggctg cgcgccaacc acatcggcat cgacggcgtg ccgctgcagg
     3421 acatcatcac gcggctgcag gccgcgtggg gcgtcgtcgc cgctgtgact tatccgacgc
     3481 ccgaagagtt cgcgccacac gcgagcccgc gcgcctgcgc cggccgaccc gaactggccg
     3541 agatccaggc gttcatcgac ttctccccgc tgctcgcctg gcggcagcgc gagaacgccc
     3601 gcctgccgga ggccatgccc atcgccgccg ccatcctctg gcggctcgcg cggcatccaa
     3661 cgtttgcgcc gctgcgcatc ggcaccaccg ttgaaatgcc cgcaatggcc gatctcggcc
     3721 gcggcgtggg tgtcgtcgtc gtgcagccgg cgaagtatgc cgatcgtcgc gatggcctcg
     3781 cgcacttcgt ccgcgactac aatgaccagg cgaagctcac caagcgccgc cgcagcggtg
     3841 gatgccagac gctggacgcc gcggcccgcc tgcccgccag gtgtgagatc gcgctccttc
     3901 gccacgccct ccgcggcgac gccgccttcg gcagcctcgg gttcaccatg atccgcgacg
     3961 gcaaaatctt cggcgccccc ctcggcgacg tgggtcatcc ccacggcttc atcgccatcg
     4021 gcagcgtggc gctgcccacc cccgacggcc ggcgggtcgg atgcgtcacc gtcaaaggtc
     4081 cgccgcagcg catcgctgga
//
LOCUS       DATIMH010000893         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4544898, whole genome shotgun sequence.
ACCESSION   DATIMH010000893 DATIMH010000000
VERSION     DATIMH010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4544898"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..2555)
                     /locus_tag="VLI90_15520"
     CDS             complement(225..2555)
                     /locus_tag="VLI90_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HSV15670.1"
                     /translation="MLRAASLLSILICFTAALARAQSAPTTAPAGENLVTMDFPADGV
                     ELSVIADVVTRELHIPILYDEQVKGKRVIVRVPTKVPERALMGILQSALRLKQLALVD
                     AEEPGWKQIVPAPNLAGVANPGPAAGEGPVTQVFVLNHGDPTKLVEIIRPFLTQPGGF
                     VQVEPSQKVLIVSDYASVVHRVELLIQQLDAQAPHVDVRFVALKEAEATQVATAVQQL
                     VRSRDAVQFGSADASGIALTADERTNQIIVIAPPSRMDDVLGLIAGMDTSTQLVTRVY
                     RLKSVTPDRVDRLIKSLIDAPTLKRGYQAGVESDSQLFVVSATATVHARIEELLRELD
                     VPAPQSQNPIQFYKLKNTKAADVLGTISGLLGESGGDTTAVPSRTTDAGTPPQITAAA
                     NPPAAGANSGGAGAPTTPSAANAQTSSLASRAPLSMPGGVSIGGNSNTQYITPALPYT
                     NESPAAASGPGVLSVKARNATVAADVNTNSIIVIAPPQVQTMYADLINRLDERRPQVQ
                     IECTIVTLDTSDGFSFGVDIGHLGGFDNSKLLSLSSFGVSTADPATGRLTPVNAPGGT
                     FALLSPRIADVVLRALATNNRARFLSAPQLLVNDNGKGKLESVAQEPFAVILDASTNQ
                     SLTSLGGLASAGTSITVEPHISEADYLQLAYSIELSNFTGAAQNGLPPPSQKNAVDSS
                     VTIPDGYTIVVGGLTTKNYTTAVNTIPVLGDIPLLKYLFGTRSKDTSDSTLFVFIKPV
                     ILRDDQFEDLKYLSSKSTQAAGIGGDFPQSEPIPMR"
BASE COUNT          431 a          816 c          946 g          508 t
ORIGIN      
        1 acgccgcgcg aaggttgtca ccggtgtcga tcatgctggg ccgcattgta ctgtgaacgg
       61 acgcgggatc gcgcgacaaa ttctggtggg tcttcaccgc ggttaagaag cgacatcatg
      121 cccgccgacg ccggtgtctg aggtacgccg aagacccggg tacgttcgcg cgaagaaccc
      181 gggtcttcgg agtacctcag acaccggcgt cgcggtttct ttactcaccg catcgggatc
      241 ggctcgctct gcgggaaatc cccgccgatg ccggcggcct gcgtgctctt gctcgacagg
      301 tacttcaggt cctcgaactg gtcgtcgcgc aggatcaccg gcttgatgaa cacgaacagc
      361 gtcgaatcgc tcgtgtcctt cgatcgcgtg ccgaacaggt acttgagcag cgggatgtcg
      421 cccagcaccg ggatcgtgtt gacggcggtc gtgtagttct tggtcgtcaa gccgccgacc
      481 acgatcgtgt aaccgtcggg aatcgtcacc gacgaatcaa ccgcgttctt ctggctgggc
      541 ggcggcaggc cattctgggc ggcgccggtg aagttgctca gctcgatgct gtacgccagc
      601 tggagatagt cggcttcact gatgtgcggc tcgacggtga tcgacgtgcc ggctgacgcc
      661 aggccgccca ggcttgtgag cgattgattg gtgctggcgt cgaggatgac ggcgaatggt
      721 tcctgcgcca cgctttccaa tttgcctttg ccgttgtcgt tcacgagcag ctgcggcgcg
      781 gaaaggaagc gggcgcggtt gttggtggcg agcgcgcgca gcacgacgtc ggcgatgcgc
      841 gggctgagca gcgcgaacgt gccaccgggg gcgttcacgg gcgtcaaccg gccggtggcg
      901 gggtcggcgg tgctgacgcc gaaggagctg agcgacagca gtttgctgtt gtcgaacccg
      961 cccaggtggc cgatatcgac cccgaaagaa aatccatcgg acgtatcgag cgtgacgatc
     1021 gtgcattcga tctgcacctg cggccggcgt tcgtccaggc gattgatcag gtccgcgtac
     1081 atcgtctgca cctgcggcgg ggcgatgacg atgatgctgt tggtgttcac gtcagcggcg
     1141 acggtggcgt tgcgcgcttt gaccgaaagc acccccggcc cgctggcagc ggcgggcgat
     1201 tcgtttgtat aaggcagcgc gggcgtgatg tactgggtgt tggaattgcc gccgatgctg
     1261 acgccaccgg gcatcgatag cggcgcacgc gatgccaatg aagaagtctg cgcgtttgcg
     1321 gcggacggcg ttgtgggtgc gcccgcgccg ccggagttcg cgccggcagc gggtggattg
     1381 gcggccgcgg tgatctgcgg tggcgtgccg gcatccgtcg tgcgcgatgg caccgcggtt
     1441 gtgtcgccgc cgctttcacc gagcaatccg ctgatcgtgc cgagcacgtc ggcggccttg
     1501 gtgttcttga gcttgtagaa ctggatcggg ttctgcgatt gcggcgccgg cacgtccagc
     1561 tcgcgcagca gctcttcgat gcgcgcgtgc accgtcgcgg tggcggagac gacgaacagt
     1621 tgcgaatcgc tttccacacc ggcctggtag ccgcgcttca gcgtcggcgc gtcgatcagg
     1681 ctcttgatca gtcgatcgac ccgatcgggc gtgacgctct tgagccggta cacgcgggtc
     1741 accagctggg tggacgtgtc catcccggcg atcagtccca gcacgtcgtc catgcgcgac
     1801 ggcggggcga tcacgataat ctggttcgtg cgctcgtccg ccgtcagcgc gatgccggag
     1861 gcgtcggcac tgccgaactg aacggcgtcg cggctgcgga ccagctgctg cacggccgtc
     1921 gccacctgcg tcgcttcggc ttcctttaat gcgacgaagc gcacgtcaac gtgcggcgcc
     1981 tgcgcgtcga gttgctggat caacagctcc acgcggtgaa cgacgctggc gtagtcgctg
     2041 acgatcagca ccttctggct cggttcaacc tggacgaacc cgccgggctg ggtgaggaac
     2101 gggcggatga tctcgacgag cttcgtcgga tcgccgtggt tcagcacgaa tacctgcgta
     2161 accgggcctt caccggcggc ggggccgggg ttcgccacgc cggccaggtt tggcgcggga
     2221 acgatctgct tccagcccgg ttcctcggca tcgaccagcg cgagttgctt caaccgcagc
     2281 gcgctttgga ggatgcccat cagcgcccgc tcgggaacct tggtgggcac gcggacgatg
     2341 actcgtttcc ctttgacctg ctcatcgtac aggatcggga tgtgcagctc gcgcgtgacg
     2401 acatcggcga tgacgctcaa ctccacgccg tcggcgggga agtccatcgt gaccaggttc
     2461 tccccggcgg gggcggtggt gggcgcggac tgcgcgcggg ccaacgcggc ggtgaagcag
     2521 atcaggatcg acagaaggga agcggcacgc agcatgggtg atacggcgaa cgtccatcgt
     2581 aaaacctgag gtggtcggaa acaacacaat tctgacgccg aggtctgagg tactggcgat
     2641 gaggtgctgg cgaagacccg ggtgcttcag cgtacgtacc cgggacttcg gcgtacccct
     2701 t
//
LOCUS       DATIMH010000894         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5704011, whole genome shotgun sequence.
ACCESSION   DATIMH010000894 DATIMH010000000
VERSION     DATIMH010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5704011"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2107
                     /locus_tag="VLI90_15525"
     CDS             <1..2107
                     /locus_tag="VLI90_15525"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSV15671.1"
                     /translation="IVMNESLSGPTFPFNLVNSNAIREAGNAGILDVVAAGNDAQNLV
                     AAPVSPATLSLSLPNVITVGAVDNVGKLAIFSNFGTGVDLAAPGVNILSTAPTSGISV
                     VTPLDPADPIAVPYGFLSGTSQAAPQVAGIIALEAAVRPNATPAQYKKALLDGVTPDQ
                     NLESVNSLPPKVSTGGIANAFNAVRNINAQFAKADAVRMGSWHNFYGLQGAYVVGEST
                     TFPSFVTASFNGVSPVILHDSTRNVVALQKVSDPSDRIAAYDASATSETIDLNFTDGL
                     LHRVSLYVADQDFRNRTETMQILDANNPAIVFDTQVASKFFHGQYFSWDLSGHVQIRI
                     TNSGGPSAVFSGIFFDAPPGNPNAFAGQDSTTLGFDWRHRFGSQGSVIVGDDNTNALP
                     DYIPVPNFDVRFASGDSASHNRILRQTIKAAVVPQKNFDPRYGVAAEWFDQDHFDISM
                     IPNDSLAHKVTLYAVDYGNRGRTERIDAIDQNTGSVLYTQDLSRFAHGTYVSFVVSGP
                     TLFRVSNTGGPDAVVSAMYFDAPPGEKLAYNGADFLTRGNWRQAGYGLTRQYDAGENM
                     PGVDQPNDVFFSSVNASRLVLQNPSSDVRAPEDVNQFSAGRVMGYAFTNTQMSFTLSI
                     ADHEQHQLALYFVDYENHHRREAITITDPTTGQVLARHRIANFAGSEYLVYQIRGSVV
                     ITINNEGSPNAVLNGVFLT"
BASE COUNT          427 a          781 c          658 g          385 t
ORIGIN      
        1 catcgtgatg aacgaaagcc tgagcggccc gacgttcccg ttcaacctgg tgaacagcaa
       61 cgcgattcgc gaagccggca acgcgggcat tctggatgtg gttgccgccg ggaatgacgc
      121 gcagaacctg gtggcggcgc cggtgtcacc ggcgactctg tcgctgtcgc tgccgaacgt
      181 gatcaccgtg ggagcggtgg acaacgtcgg caagctggcg atcttctcca acttcggcac
      241 cggtgtggat cttgccgcgc cgggcgtgaa catcctttcg accgccccca ccagcggaat
      301 ttccgttgtc acgccgctcg atccggccga tcccattgcc gtgccttacg ggttcctcag
      361 cggcacatcg caggcggccc cgcaggtcgc ggggatcatc gcgctcgaag ccgccgtgcg
      421 acccaacgcc acgccggctc aatataagaa ggcgctgctg gatggcgtga cgccggatca
      481 gaacctggag tcggtcaact cgctgccgcc caaggtgagc accggcggca tcgccaacgc
      541 gttcaacgcc gtccgcaaca tcaacgcgca gttcgccaag gcggacgccg ttcgcatggg
      601 cagctggcac aacttctacg gcctgcaagg cgcgtacgtc gttggtgaat cgaccacgtt
      661 cccgtcgttc gtgaccgcca gcttcaacgg cgtcagcccg gtcatcctgc acgactcgac
      721 ccgcaatgtc gtcgcgctgc agaaggtgtc ggacccgagc gatcgcatcg ccgcttacga
      781 cgcatcggcc accagcgaaa cgatcgatct gaacttcacc gatggattgc tccatcgcgt
      841 gtcgctgtac gtggccgacc aggacttccg caaccgcacc gagacgatgc agatcctgga
      901 cgccaacaat cccgccatcg tcttcgacac gcaggtcgcg tcgaaattct tccacgggca
      961 atatttctcc tgggacctca gcgggcacgt gcagatccgg atcaccaatt ccggcggccc
     1021 cagcgccgtc ttcagcggta tcttcttcga tgccccgccg ggcaacccca acgccttcgc
     1081 cgggcaggac agcacgacgc tcggcttcga ctggcgccac cgcttcgggt ctcaaggctc
     1141 ggtcatcgtc ggtgacgaca acaccaacgc gctgcccgat tacatccccg tccccaactt
     1201 cgacgtgcgc ttcgcctccg gtgattcggc cagccacaat cggatcctgc ggcagaccat
     1261 caaggcggcc gtcgttcccc agaagaactt cgacccgcgt tatggcgtgg ccgccgagtg
     1321 gttcgaccag gaccacttcg acatcagcat gattccgaac gattcgctgg cgcacaaagt
     1381 gacgctgtat gcggtggact acggcaaccg cgggcgcacc gaacgcatcg acgcgatcga
     1441 tcagaacacc ggcagcgtgc tgtatacgca ggacctgtcg cgcttcgcgc acggcacgta
     1501 tgtcagcttc gtcgtcagcg gcccgaccct gttccgcgtg agcaacaccg gcggcccgga
     1561 cgccgtggtc agcgcgatgt acttcgacgc gcccccgggt gaaaagctgg cctacaacgg
     1621 cgctgacttc ctcacccgcg gcaactggcg ccaggccggg tatggcctca cccgccaata
     1681 cgacgccggc gaaaacatgc ccggcgtcga tcagcccaac gacgtcttct tcagctccgt
     1741 caacgccagc cggctggttc tgcagaaccc gtcgtccgac gtgcgcgctc ccgaagacgt
     1801 gaaccagttc agcgccggcc gcgtgatggg atacgcgttc acgaacacgc agatgtcgtt
     1861 cacgctgtcg atcgccgacc acgagcagca ccaactggcg ctatacttcg tggactacga
     1921 gaaccaccat cgccgcgagg cgatcacgat caccgacccg acgacggggc aggtgctggc
     1981 gcggcaccgc atcgccaact tcgccggcag cgaatatttg gtctatcaaa tccgcggcag
     2041 cgtagtgatc acgatcaaca atgaaggatc gccgaacgcg gtgttgaacg gcgtgttcct
     2101 gacgtgatgc gcgtccgctc caagagctct cgataaaccc ccacgccggg ttcggagcgt
     2161 actcgcgacg acccggatct cttgtgcatt gaaaagcatc cgggtcgtcg gagtctcctt
     2221 cgacttcgct caggacaggc ctccgagccc g
//
LOCUS       DATIMH010000895         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4257661, whole genome shotgun sequence.
ACCESSION   DATIMH010000895 DATIMH010000000
VERSION     DATIMH010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4257661"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1216)
                     /locus_tag="VLI90_15530"
     CDS             complement(107..1216)
                     /locus_tag="VLI90_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSV15672.1"
                     /translation="MRDFISKRAINVDASGIRKVFDLAARMKDPINLSIGLPDFDVPA
                     PVKDAAIESIKAGQNRYTQTQGIAPLRERLRADLSKEIGRDVGEVLITSGVSGGLLLA
                     ILAVLDPGDEAIFLDPYFVMYKHLITMAGGRSAIVDSYPDFRFHADRVEAAVTSRTKL
                     LILNSPSNPTGVVMTEAEVRAAVDLAKKHDLLILSDEIYEPFLYDAQRGLPSAAKLYE
                     NTIILRGFSKSHAMTGWRLGYAAGPVDVIAQMTKLQQYTFVCAPSPLQYAALKALDVP
                     MDDAVAAYRRKRDIVFDGLSKRFELVKPQGAFYIFPKAPAQTTASEFVARAIENNVLI
                     IPGNVFSERDTHFRISYATTDEKLRQGVEVLNALA"
     gene            complement(1341..1727)
                     /locus_tag="VLI90_15535"
     CDS             complement(1341..1727)
                     /locus_tag="VLI90_15535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HSV15673.1"
                     /translation="MSQHRSLKSASSLVRHRNVLTRAERLDKLQSEEKWTDSKSVYGL
                     PKVAHRKMELAKPVKEEAAEGAEGAAPAAGAAAPAAGAKGAAPAAKGGAGAKGAAPAA
                     GAKGAGAAKGAAPAAGAKAAAPAKKK"
     gene            2097..2294
                     /locus_tag="VLI90_15540"
     CDS             2097..2294
                     /locus_tag="VLI90_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15674.1"
                     /translation="MKTSSRNVVIRRFCAIAVIVAASLCIGGCHHENGDDNRNANAVD
                     RRLTPTNVTAMGAMLVVTDAA"
     gene            2552..3541
                     /locus_tag="VLI90_15545"
     CDS             2552..3541
                     /locus_tag="VLI90_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV15675.1"
                     /translation="MAVVGCGGMGGQHIGSIAAHPDATVAALVDVRPAAAQQKLKDLL
                     HDDPSVRQYESLDAMLSDPPPGLRGVVIATPHTLHFAQSVACLQRGLDVLVEKPMVTS
                     SAHARELKSTIERTGRQFQVAFQAPFSREFAYIRQQLQSGALGELQTITAFSCQGWRK
                     ACANTWRHDPKLSGGGQMYDTGAHLFNSIAWLVDRPVVEVFCITDNKGLAVDINAAMT
                     IRWEGNVLGSVTISGNTPGWQEGIWISGDRGRIATGIHGGRLEQFDEKGPVKYPLVTQ
                     PHQTPVGNFIDCLLGKAQPCCPVRYGILHSWLMDALYESAREGRAVKPSAPPL"
BASE COUNT          660 a         1152 c         1157 g          693 t
ORIGIN      
        1 gatttgttta aatcgaattt gcgtagcggg cgcctcgcag gggcacgtgt ttcttccaca
       61 aaacacgtgc ccctgcgggg caaccgctac gcgtggaaac gaatagtcac gccagcgcgt
      121 tcagcacctc tacaccctga cggagcttct cgtccgtcgt tgcatagctg atgcggaaat
      181 gcgtgtcgcg ctcgctgaag acgttgccgg ggatgatcag gacgttgttc tcgatcgcgc
      241 gggcgacgaa ttcgctcgcg gttgtctgtg ccggcgcttt ggggaagatg taaaacgccc
      301 cctgcggctt gaccaattcg aaccgcttcg acagcccgtc gaacacgatg tcgcgcttgc
      361 ggcgatacgc cgccacggcg tcgtccatgg gcacatcgag cgccttcagc gccgcatact
      421 gcagcggcga cggcgcgcac acgaacgtgt actgctgcag cttggtcatc tgcgcgatca
      481 catccaccgg ccccgcggcg tagccgagcc gccagccggt catcgcgtgg ctcttgctga
      541 agccgcgcag gatgatcgtg ttctcgtata gcttcgccgc gctcggcagg ccgcgctgcg
      601 cgtcatacaa aaacggctcg taaatctcat ccgacaggat cagcagatcg tgcttcttcg
      661 ccagatcgac ggccgcgcgg acttcggctt cggtcatcac cacgccggtg ggattgctcg
      721 ggctgttgag gatcagcagc ttggtgcgcg aggtgacggc ggcctccacg cgatcggcgt
      781 ggaagcggaa gtcggggtag ctgtccacaa tggccgaccg gccgccagcc atcgtgatca
      841 ggtgcttgta catcacgaag tacgggtcga ggaagatcgc ctcatcgccg ggatcgagca
      901 ccgccaggat cgccaacagc agcccgccgc tcacgccgga agtgatcagc acttcaccga
      961 cgtctcggcc gatttccttc gagagatccg cgcgcagccg ctcgcgcagc ggggcgatgc
     1021 cctgcgtctg cgtgtagcga ttctggccgg ccttgatcga ttcgatcgct gcgtccttca
     1081 ccggtgcggg gacgtcgaaa tcgggcaacc cgatcgacag gttgatcggg tccttcattc
     1141 gagccgcaag gtcgaacacc ttgcgaatgc cggaggcgtc aacgttgatc gctcgcttgg
     1201 aaatgaaatc gcgcatgatg atcaaacaga aaaaggtaaa cggaaaatgg caaatggatc
     1261 agaaaggctc gatcgccttt cgatgttcca cttgccattt cccattatcc atggcgacag
     1321 caacgctgtc gcgacacaga ttacttcttt ttcgccggag cagccgcctt cgcgcccgcg
     1381 gccggggcag cgcccttggc ggcgccggcg cccttggcgc ccgcggccgg agcagcgccc
     1441 ttcgccccgg ctccgccctt ggcggcaggc gcagcgccct tcgcgcccgc agccggcgcg
     1501 gcagcgcccg cggcaggagc agcgccttcg gcgccctcgg ctgcctcttc cttcaccggc
     1561 ttggccaatt ccatcttgcg atgcgcgacc ttcggcaggc cgtacacgct cttgctgtcg
     1621 gtccacttct cttcggactg aagcttatcc aaccgctcgg cccgcgtgag cacattgcgg
     1681 tgccgcacca gcgaactagc actcttcaaa ctgcgatgct gtgacatcag gaacctctcg
     1741 cgttgcacgc gtcgattgtc gaaattcaaa tcccgcccgg cgcgggggaa ggatgatact
     1801 gacatgccgc ggggttcaca acccggtgga tgtaaggtcg gagcgggggt tgagtcggcc
     1861 cgcgagcccg gatgatttca actctagtca ttttaagatg ttatatctga cactcggctt
     1921 atgaatcacc tgtgactgac atttaatagc cggggccgga ggccggtcct ttcggacgac
     1981 cagcgattct cgaacgacgt cccgcggcag agccgtgggc gttgtccgaa gggatttcgt
     2041 tcaatgacag tttttccccg atgcgtcttc ttgcggcggc ggactatggt gccgcgatga
     2101 aaacttcatc tcgcaacgtc gtgattcgcc gcttctgcgc catcgctgtg atcgtggccg
     2161 ccagcttgtg cattggcggc tgtcatcatg agaacgggga tgacaaccgc aacgccaacg
     2221 cggtggaccg ccggctgacg ccgacgaacg tgaccgcgat gggcgcgatg ctcgtcgtca
     2281 ccgacgcggc gtgatcgctg caccgtcatc tgacgatctc gatcgactcg cgcgacatac
     2341 tctgccccgc aagccgcgcc atttgttgct gcgcctgcga cgttatcatg atcccaccac
     2401 gccgggttcg gaggtactcc gacgacccgg atcttttcgc gcgcctgaga agtagaacct
     2461 cgcttcgtcg gagtacctcc gaacccggcg tgggtggtga tgacgatgtc cggatcagac
     2521 ttggagatct cgcttgccgg aaaaaatcaa attggcggtc gtgggttgtg gaggcatggg
     2581 tggacagcac atcggctcga tcgcggcgca tcccgatgcg acggtcgcgg cgctggtgga
     2641 cgttcggccg gcggcggctc aacagaagct caaggacctg ctgcacgatg atccttcggt
     2701 ccgccagtac gaatcgctcg acgcgatgtt gagcgatccc ccgccggggc tgcgcggcgt
     2761 ggtcatcgcg acgccgcaca cgttgcactt tgcccagtcg gtcgcgtgcc tgcagcgcgg
     2821 gctggacgtg ctggtcgaga agccgatggt gaccagctcc gcccacgcgc gcgagttaaa
     2881 gtccacgatc gagcgcaccg gccggcagtt tcaggtggcg ttccaagcgc cgttctcgcg
     2941 cgagttcgcg tacatccgcc agcaactgca atccggcgcg ctcggtgaat tgcagacgat
     3001 caccgcgttt tcctgccagg gctggcggaa ggcgtgcgcc aacacctggc ggcacgaccc
     3061 caagctctcc ggcggcgggc agatgtacga caccggcgcg cacctgttca actccatcgc
     3121 ctggctggtc gatcggcccg tcgtcgaggt cttctgcatc accgacaaca aaggcctggc
     3181 cgtggacatc aacgcggcca tgacgatccg ctgggaaggc aacgtgctgg gcagcgtgac
     3241 gatcagcggc aacacacccg gctggcagga aggcatctgg atcagcggcg accgcgggcg
     3301 aatcgccacc ggcatccacg gcggccggct tgagcagttc gacgaaaaag gcccggtgaa
     3361 atatccgctg gtcacccagc cgcaccagac gccggtcggg aatttcatcg actgcctgct
     3421 gggcaaggcc cagccgtgtt gcccggtgcg gtatgggatt ttgcatagct ggttgatgga
     3481 tgcgctgtac gaaagtgcgc gcgagggacg agcggtgaag ccgagcgcac cgccgctgtg
     3541 atggtcatcc tgggaggcgg tgagttgagc ggagcacgta ctcgtgcgtc gtcgcaggat
     3601 ctggcagaga agtagatgaa ggattagtca cgacgaccag acagcagtcg cttgcgtctt
     3661 cg
//
LOCUS       DATIMH010000896         1781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3681973, whole genome shotgun sequence.
ACCESSION   DATIMH010000896 DATIMH010000000
VERSION     DATIMH010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3681973"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="VLI90_15550"
     CDS             <1..1236
                     /locus_tag="VLI90_15550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV15676.1"
                     /translation="CSFVSETPAGTVGGVEKIQYKLAGGATKQNNFCSRTFENTVWHC
                     EAVYKAMKARPDIRPDLIVGHSGFGSTLFLRELYPDVPCINFFEYYYNPHDPDNDMDF
                     RQDLNWHIPDEKYLRSRCRNAMILLDLQNCNAAYAPTNFQRTRFPSEYLDKIQVIFDG
                     VDRSVYHGFNEKLRPPLLTRGPRTIAGVNVPANTRIVTYVSRGFESMRGFDIFMRSAK
                     RIAEQFPDVIFIVVGSDRIAYGGDEGYIQGHKTFREWVLSRETYDLSKFAFVGRIPPL
                     ELAKLLATTDLHIYLTVTFVLSWSMMDAMGCGAVVLGSATSPVKEMIRDGENGLLADF
                     FNPDEFAQKAIPVLKDPDAFRPLGRAAEQMIAEQYSLEAVLPKMLKLYEDTLSKRPAV
                     PNRPRRAPAQDNQANRFSG"
     gene            complement(1256..>1781)
                     /gene="pyrF"
                     /locus_tag="VLI90_15555"
     CDS             complement(1256..>1781)
                     /gene="pyrF"
                     /locus_tag="VLI90_15555"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSV15677.1"
                     /translation="EIATPDAITVNPMLGLDTLEPFTKTAAELGKGLFVLVRTSNPGS
                     AELQDVKLADGRTWSEMLAEKLAKVADTMTGASGWSSLGAVVGATQTHTMVSLRQRMP
                     KSIFLLPGYGTQGATAEMTRAAFVDGRGAIVSASRSILYAHKDAKYAGEGDWTRCVLQ
                     ATLDMKADLRPVLG"
BASE COUNT          341 a          562 c          535 g          343 t
ORIGIN      
        1 tgcagcttcg tcagcgaaac ccccgcgggg accgtgggtg gggtcgagaa gatccagtac
       61 aagctggccg gcggggcgac gaagcagaac aacttttgtt cgcggacctt cgagaacacg
      121 gtgtggcact gcgaggctgt ctacaaggcg atgaaggcgc ggccggacat ccggcctgac
      181 cttatcgtcg gtcacagcgg gttcggttcg acactcttcc tgcgcgagct gtatcccgat
      241 gtgccgtgca tcaacttctt cgagtactac tacaacccgc acgaccccga caacgacatg
      301 gatttccgcc aggacctgaa ctggcacatc cccgacgaaa agtatttacg ctcgcgctgc
      361 cgaaacgcga tgatcctgct cgacctgcag aactgcaacg cggcgtacgc gccgaccaac
      421 ttccagcgca cgcgctttcc cagcgaatat cttgacaaga tccaggtgat cttcgatggc
      481 gtcgatcgct cggtctatca cggcttcaac gaaaagctcc ggccaccgct gttgacacgc
      541 gggccccgca cgatcgcggg tgtgaacgtg ccggcgaaca cgcgcatcgt cacgtacgtc
      601 agccgcgggt tcgaatcgat gcgcgggttc gacatcttca tgcgcagcgc caagcgcatc
      661 gccgagcagt tccccgacgt catcttcatc gtggtgggca gcgaccgcat cgcctacggc
      721 ggggacgagg gatacatcca gggccacaag acgttccgcg aatgggtgct gtcgcgcgag
      781 acgtatgacc tgtcgaagtt cgcgttcgtc ggtcgcatcc cgccgctgga attggcgaag
      841 ttgctcgcga caacggacct gcacatctac ctgacggtga cgttcgtgct gtcgtggagc
      901 atgatggacg cgatgggctg cggcgcggtg gtgcttggct ccgcgacatc gccggtgaaa
      961 gaaatgatcc gcgacggcga gaacggcctg ctggccgact tcttcaaccc cgacgagttc
     1021 gcgcaaaagg cgatcccggt tctaaaggat ccggatgcat tccgtccgtt aggtcgcgcc
     1081 gccgagcaga tgattgccga gcagtacagc ctggaagcag tgcttcccaa gatgctgaag
     1141 ctctacgaag acacgttaag caaacgtccg gcagtgccca accgtcctcg ccgtgcccct
     1201 gcgcaggaca accaggcgaa ccgcttcagc ggttagcgtc acgctcgagg aaagatcacc
     1261 ccagcaccgg ccgcaagtcc gccttcatat ccagcgtcgc ttgcagcacg caacgcgtcc
     1321 aatccccttc gcccgcgtac ttcgcgtcct tgtgcgcgta caagatactc cggctcgccg
     1381 agacgattgc gccgcggcca tcgacgaaag ccgcccgcgt catctcggcc gttgcgcctt
     1441 gcgtgccgta gcctggcaag aggaagatcg atttcggcat tctctggcgg aggctgacca
     1501 tcgtatgggt ctgggttgcg ccgacgaccg cgcccaggct gctccagccg cttgcgccgg
     1561 tcatggtgtc tgctactttt gcaagttttt cggcgagcat ttcgctccag gttcggccat
     1621 cggctaattt cacatcctgc aactcggctg atccggggtt gctggtgcgg accaaaacga
     1681 agagaccttt gcccagttcg gcggcggttt tggtgaaggg ttccagcgta tccaggccca
     1741 gcatcggatt aaccgtgatc gcatcgggtg tggcgatttc g
//
LOCUS       DATIMH010000897         5696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3682277, whole genome shotgun sequence.
ACCESSION   DATIMH010000897 DATIMH010000000
VERSION     DATIMH010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3682277"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1244)
                     /locus_tag="VLI90_15560"
     CDS             complement(39..1244)
                     /locus_tag="VLI90_15560"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSV15678.1"
                     /translation="MGDVVIDYARPPLAARERAVGYIPELDTFRTVAILLVMSLHWLP
                     ERSWINRIQTGGDFGVYLFFVLSGFLITRILMRCREDAERGISSRGFALRQFYARRFL
                     RIFPAYYLLLLVGVALNSPGLRAGFAWHAAYLSNVYFYHRRAFGGPASLFWTLSVEEQ
                     FYLLWPLAILFIPRRAIQPFVVATLVLGTILHGAARLMHPWAGILTPACMNFLAAGAL
                     IAVCESNLCGGAAKRERLLRLFAVLSGALALAAIVLWFVPSVPKKLDWERVVIQTGAS
                     FAFAWSIARAADGVGGALGIVLRFAPLVYLGRISYGLYIYQQFVVHWLNWLPPRVARH
                     AGGGSAITAFASTFTARFLATVALASLSWFLYEKPLNDLKRFFPYARKPRPLALEERG
                     VPLPAATGS"
     gene            complement(1564..3729)
                     /locus_tag="VLI90_15565"
     CDS             complement(1564..3729)
                     /locus_tag="VLI90_15565"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSV15679.1"
                     /translation="MFETLEDRTLMSTYYVNTGGNDSNSGTSSSAPWRTISKVNSASL
                     KAGDQVLFQGGQTFSGGLRPKNGGNSSSAITFSSYGNGKATIKSGGSDGFYALSKSGL
                     WINNLDFVGSPSGKNHDGVRFETNSGRQYNVRVTNSSISGYGFAGILVLGDRNGIGFT
                     NVQITGNEISNNVTSGIQFSAVSRNVHQSVYIARNNVHDTYGDGSSINTGNGIMLGGL
                     AGGTIEWNTSYHNGARGGNGGVGIWAYESTNVLMQYNVSHDNKTTRGMDGDGFDFDAD
                     TQNSTMQYNYSYNNDGAGFMLDQWRANANFTNNIIRYNISQNDGRNNNYGGICVFGQI
                     ENSTIYNNTVYLSGGPGGAVGVRIKRSALGGGGPVNLKIGNNIIETSGKQLLNITSNA
                     TAGVKFVGNTWYNAGGTPSYSYAGKSYSSLSSWHSGTGQDSNGLQTNPMLNAPGAGQA
                     LSNASSLSSLTAYKLKSNSPLINRGINIASVLGASISHDFFGDSLPQGGAYEFGVNEV
                     GIIGKPPLPAGDPAPAPAPSPTPTPTPSSSLPSDWSSANIGSVGQAGSSSYTTNGFKY
                     TISGGGADIYGANDSFRYSYTSLSGDGSIIARVDSMTAANDWAKAGVMIRNGNAANAA
                     EVSMMLNVNKTAQFERRWWAGDTTHTTSTSANSDAWVKLVRTGDTFNGYISSDGNNWT
                     LVEHTTVKMNSNVEIGLGVTAHDNTKLETAVFSNVKVVH"
     gene            complement(4531..>5696)
                     /locus_tag="VLI90_15570"
     CDS             complement(4531..>5696)
                     /locus_tag="VLI90_15570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSV15680.1"
                     /translation="AATAARSIAAKANIVQQEEAFLAALLMDIGMLVLDQALGERYGA
                     VHEKIKAHEELMAVEKADLSGDHAEVGGWLAEQWKLPPLLSTPISQHHNSASVQDPTL
                     YKLTEVVSLGGRCADVFVDENAAPAIAEVRARFAKLCNLTEADSDALLNEIGTRTKEV
                     ASLFEINLGSAVDFEAVLKRANEALVEITLQSQMQASQLQEQNVELKRAATTDALTGL
                     ANRAHLDQFLAEQFTDARVAGQPVSLLLMDVDKFKSVNDKHGHPAGDQVLRSLGKLLN
                     SAARPQDLAARYGGEELCLVLPGTPKPLAATIAESVRRAISAKPVALPGTALNVTVSI
                     GVATFEPSGPFKEVAHLLKAADLAVYAAKKSGRNCVRVFTLPAAPAPPKAKVA"
BASE COUNT         1052 a         1801 c         1759 g         1084 t
ORIGIN      
        1 cgctcccaac ccggcgtggg agcggaccgg aaactacctc aggatcccgt cgccgcgggc
       61 agcggcacgc cgcgttcttc aagcgctagc ggtcgcggct tgcgcgcgta ggggaagaat
      121 cgcttcaaat cgttcagcgg cttctcgtac aggaaccatg acaggctcgc gagcgcgacc
      181 gtcgccagga agcgagcagt gaaggtgctg gcgaacgcgg tgatcgccga gccaccgccg
      241 gcgtggcggg caactcttgg ggggagccag ttgagccagt gcacgacgaa ttgctgatag
      301 atatataggc cgtagctgat ccggcccagg tagacgagcg gcgcgaagcg caacacgatg
      361 ccgagtgcgc caccgacgcc atcggcggct cgggcgatgc tccacgcgaa cgcgaacgaa
      421 gcgccggtct ggataaccac gcgttcccag tccagcttct tcggcaccga cggaacgaac
      481 caaagcacga tcgcggccag cgctagcgcg ccgctcagca ccgcgaacaa tcgcagcagt
      541 cgttcgcgct tcgcggcacc gccgcacaag ttgctttcgc acaccgcgat cagcgccccc
      601 gccgccagga agttcatgca ggccggcgtg aggatgccgg cccacgggtg catcaggcgc
      661 gccgcgccgt gcagtatcgt gccgagtact agcgtcgcca cgacgaacgg ctgaatcgcc
      721 cgccgtggga tgaacaggat cgccagcggc caaagcaggt agaactgctc ttcaacggac
      781 aacgtccaga acagcgaagc cggcccaccg aacgcccggc ggtgatagaa gtagacgttc
      841 gacagatacg ccgcatgcca cgcgaacccc gcgcgcagcc cgggcgaatt cagcgccacg
      901 ccaaccagca gcagcaggta atatgccggg aaaatccgca gaaatcgacg agcgtaaaac
      961 tgccgcagcg caaagccgcg cgacgagatg ccgcgttccg cgtcttcgcg gcagcgcatc
     1021 aagattcgcg tgatcaagaa gccgctcagg acgaagaaca gataaacgcc gaaatcgccg
     1081 ccggtctgga tgcgattgat ccagctgcgc tcgggcagcc agtgcagcga catcacgagc
     1141 aggatcgcga cggtgcgaaa cgtgtcgagt tcggggatgt acccaaccgc tcgctcgcgg
     1201 gcagcgagcg gcggacgtgc atagtcgatg acaacatcac ccatcgctgc gcatattatc
     1261 ggcccgatgc gccagcgact acagccggtg atcctattga agcaaccgcc gacgccgggc
     1321 catgcgtgcc tcggaagttg aaacgaaaac aggtgcccct gcgcggctac cggtacgcga
     1381 tggccacgcg cgccaggcgc gcgccatctg ccggcgattt gatgcgtggc aatgagtcgg
     1441 ggttacacga gcaggcgccg tccattccaa cagccattcg acaaatgaag atggccaccc
     1501 tcgcatgagg gtggccatcg gtgtttgcga aattgtcgct ggttggactc ccggcaggtg
     1561 cgtttagtgc acgaccttca cgttgctgaa cacggccgtt tccagcttcg tgttgtcgtg
     1621 ggcggtgacg cccaggccga tctccacgtt gctgttcatc ttgaccgtcg tgtgttcgac
     1681 gagtgtccag ttgttgccgt cgctggagat gtagccgttg aaggtgtcgc cggtgcggac
     1741 cagcttcacc caggcatcgg agttggcgct ggtgctggtc gtgtgggtcg tgtcgccggc
     1801 ccaccagcgg cgctcgaact gagcggtctt gttgacgttc agcatcatcg acacttcggc
     1861 ggcgttggcg gcgttgccgt tgcggatcat gacgccggcc ttcgcccagt cgttggcggc
     1921 ggtcatgctg tccacgcggg cgatgatcga accgtcgccg ctgaggctcg tgtacgaata
     1981 gcggaacgaa tcgttggcgc cgtagatgtc ggccccaccg ccgctgatcg tgtacttgaa
     2041 accgttggtg gtatacgacg aactgccggc ctgaccgacg ctgccgatgt tggcgctgga
     2101 ccaatcgctg ggcagcgagg acgacggcgt gggagtcggc gtcggcgacg gcgcgggcgc
     2161 cggggccgga tcaccggcgg gcagcggcgg tttgccgatg atgcccactt cgttcacgcc
     2221 gaactcgtac gcgccacctt gcggcagcga atcgccgaag aagtcgtggc tgatcgacgc
     2281 gcccaggacc gacgcgatgt tgatcccgcg gttgatcagc ggcgaattgc tcttcagctt
     2341 gtatgccgtc aggctgctca ggctcgacgc attgctcagc gcctggccgg cgccgggagc
     2401 attgagcatc gggttggtct gcaggccatt ggaatcctgg cccgtgcccg aatgccacga
     2461 gctgaggctc gagtacgact tgccggcata gctgtagctc ggcgtgccgc cggcgttgta
     2521 ccaggtgttg ccgacgaact tcacgcccgc cgtcgcgttg gacgtgatgt tcagcagctg
     2581 cttgccagac gtctcgatga tgttgttgcc gatcttcaga ttcaccggcc caccaccgcc
     2641 gagcgccgag cgcttgatgc gaacaccaac cgcgccaccg gggccgccgg aaaggtaaac
     2701 ggtgttgttg tagatggtgg agttttcgat ctgcccgaac acgcagatgc cgccgtaatt
     2761 gttattgcgg ccgtcgttct ggctgatgtt gtagcgaatg atgttgttgg tgaaattcgc
     2821 gttggcgcgc cactggtcca gcatgaaacc ggcgccatcg ttgttgtacg agtaattgta
     2881 ctgcatcgtg gagttctggg tgtcggcgtc gaagtcgaag ccgtcgccgt ccattccacg
     2941 ggtggtcttg ttgtcgtgcg agacgttgta ctgcatcagg acgttggttg attcatacgc
     3001 ccagatgccc acgccaccgt tcccaccgcg ggcgccgttg tgatacgagg tgttccactc
     3061 gatcgtgccc ccggccagcc cgccgagcat gatgccgttg ccggtgttga tgctcgaccc
     3121 gtcaccgtag gtgtcgtgca cgttgttccg ggcgatgtaa acgctctggt gaacgttgcg
     3181 cgagaccgcc gagaactgaa tgcccgacgt cacgttgttg ctgatttcgt tgccggtgat
     3241 ctggacgttg gtgaacccga tgccgttgcg atcgcccagg acgaggatcc cggcgaagcc
     3301 gtaaccgctg atcgaggagt tggtgacgcg aacgttgtac tgccggccgc tgttggtttc
     3361 gaagcggacg ccgtcgtggt tcttaccgct gggggagccg acgaagtcca ggttgttgat
     3421 ccacaggccg ctcttgctca gggcgtagaa cccgtcgctg ccaccggact tgatggtcgc
     3481 cttaccgttg ccatagctgc tgaaggtgat cgcgctgctg gaattcccac cgttcttggg
     3541 acgaaggcca ccgctgaagg tctggccacc ttggaacagg acttggtcgc cggccttcag
     3601 gctggcggag ttgaccttgc tgatcgtgcg ccacggagcc gagctgctcg tgccgctgtt
     3661 ggagtcattg ccgccggtgt tgacgtaata cgtcgacatc aacgtgcggt cttcgagcgt
     3721 ctcaaacaac ggcaaagcgt tgtcgtcgcg acggttatcg cggccggcat tggaacgggg
     3781 cgtcgtgcca agggactgag ccaagacacg gaatgtccgt gtgagcggaa acagcatctc
     3841 tagttactcc tcgcgcaaaa aaagcgagtt acgattgatt ggggtgaatg ggccgtttag
     3901 aacgaacccg cccgcatacc ccagtaaaag gaaacctgct gctgaggaac cgtcggttca
     3961 cgtaccccgg cgcgaggtac aaccctgacg tttcgcagct gtcgcggacc tacggaactt
     4021 gacagctcag accaggcttc ctgccagtaa gtgccctagc aaggcagatg gcaatatgca
     4081 gatcgatcta gctcatgcaa ccagacccgt ggcgaaaaat aaaaaatact tcgccgtcgg
     4141 ttgtcaagtc gcatcgattc agccacccgt cgcgcgaaac ttcactatct cccgatgaat
     4201 ctgggctaac tcgcgaaccc attccatcgc gcttcgtagg ttctggctga cttgatcgat
     4261 cgctctgcct tagcagtatt gctatcggcg gtgttattag ctgtcttctc tcttttcacc
     4321 cacttcacaa gaaaattgtc ctccacccca ctgcgcgtgt tgaaacatgc cggttgtgcg
     4381 gggggcgaaa acggcgcgga cgcgcggctc tggtcccctc tcccacgtac acgtggagag
     4441 ggttagggtg agggtgaggg tgatttaatg cttagcggga gaagattcca gccagagaat
     4501 tgaagctgca gtgtcgccga gaaatgcaat tcaagcgact ttcgcctttg gcggcgcggg
     4561 cgcagccgga agggtaaaca cccgcacaca attccgcccc gacttcttgg cggcatacac
     4621 cgcgaggtcg gcggccttca acagatgggc gacttccttg aacggcccgc tcggttcgaa
     4681 cgtcgccacc ccaatgctca ccgtgacgtt caacgccgtc cccggtagcg ctaccggctt
     4741 ggccgaaatc gcccgacgga cgctctcggc gatcgtcgcc gccagcggtt tcggtgtccc
     4801 cggcagcacc aggcaaagct cttccccgcc atagcgcgcc gccagatcct gcggccgcgc
     4861 cgcggaattc aaaagcttgc ccagcgatcg cagcacctgg tcccccgccg ggtgaccgtg
     4921 cttgtcgttg accgacttga acttgtccac gtccatcagc agcagcgaca ccggctgacc
     4981 cgccacccgc gcatcggtga actgctcggc caggaattga tccaggtgcg cgcgattggc
     5041 gagccccgtc agcgcatcgg tcgtcgccgc ccgcttcagc tcgacgttct gctcctgcaa
     5101 ctgcgacgcc tgcatttgcg actgcagcgt gatctccacc agcgcttcgt tcgcgcgctt
     5161 cagcaccgcc tcgaaatcca ccgccgatcc aagattgatc tcgaacagcg acgccacttc
     5221 ctttgtccgc gtgccgatct cgttcagcag cgcatcgctg tcggcttccg tcaggttgca
     5281 caacttggca aaccgcgccc gcacctcggc aatcgccggg gccgcgtttt catcgacgaa
     5341 cacgtccgcg cagcgcccgc ccagcgacac gacttctgtc agcttgtaca gcgtcggatc
     5401 ctgcaccgag gccgagttgt gatgctgcga gatcggcgtc gaaagcagcg gcggcagctt
     5461 ccactgctcc gccagccacc cgcccacttc cgcatgatct ccactgagat ccgccttctc
     5521 caccgccatc agctcttcgt gcgctttgat cttttcgtgc accgcgccgt agcgctcgcc
     5581 cagcgcctgg tccagcacca gcatgccgat gtccatcagc agcgccgcca ggaacgcctc
     5641 ttcctgctga acgatgttcg ccttcgccgc gatcgaccga gccgccgtcg ccgcgt
//
LOCUS       DATIMH010000898         6664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1953704, whole genome shotgun sequence.
ACCESSION   DATIMH010000898 DATIMH010000000
VERSION     DATIMH010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953704"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VLI90_15575"
     CDS             complement(<1..357)
                     /locus_tag="VLI90_15575"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSV15681.1"
                     /translation="MLLLAASCKVGPDYHPPSQAMPASWESPPTTRASVPVQAPVAVD
                     HWWATFDDPMLNSLVERALASNLTLEAATERIRGSRAAIGIVSAGLWPGVNGTADYAR
                     AGGGSSKWGSQWRAGLD"
     gene            complement(610..2787)
                     /gene="ligA"
                     /locus_tag="VLI90_15580"
     CDS             complement(610..2787)
                     /gene="ligA"
                     /locus_tag="VLI90_15580"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSV15682.1"
                     /translation="MPTATHAKQRIEQLRQELNHHNYLYYVQSRPAVSDREYDRLMQE
                     LIDLERANPDLIAPDSPTQRVGGEVQTELKPVRHAVPMMSIDNTYSEAEVRAFDERVR
                     KALDGQQPSYVLEPKIDGTSISLRYEGGKLVLAATRGRGNVGDDVTINARTIKSIPLT
                     LKDTPPKILEVRGEVYMDNDDFQRVNKEIEAEGEEPYANPRNLTSGTLRRLDPKIVAK
                     RRLRFLAHGNGQVEPMPAKSYWEWTQMLGQWGFPLPKEVSHVPGVDAALKFIHQFEKL
                     RPKLPYMTDGMVMKVDAFAHRDQLGATSKAPRWVIAYKYETEQQPTVLKDVDWQVGRT
                     GQLTPVGKLEPVFIGGVTVSNVTLHNIDQIQRLGIHIGDTIVVERSGEVIPYVVEVHK
                     DKRPSGAKSVQPPKKCPACATPVVREALTEGHAAYRCVNEACDVFFERKRVKRAKLPT
                     DCPVCGKPVELLDSGIDILCPNDLCPGRMKEAIRYFCGRSQMDIEGLGDVLVDQLVER
                     GLVRTFADLYKLKPQDLAGLTSEVEQGGKSVTRTVGDKVAQKVVKNIDNSRNQPLDRL
                     LAGLGIRHVGNRVAHVLASHFGSLDAIAGATQEQLAAVHEIGEVIADSVHDYFHNDAG
                     KLVVKALKNVGVDPKMEKPAGDGAALPLSGQTVVVTGTLPTLGRQEIEELILKLGGKA
                     SGSVSKKTSFVVAGESAGSKLDKAKELGVPVLDEAGFLKKIGR"
     gene            complement(3056..5053)
                     /locus_tag="VLI90_15585"
     CDS             complement(3056..5053)
                     /locus_tag="VLI90_15585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV15683.1"
                     /translation="MLSLTHLEAPAPVVRQRVRAQRSAPSALRRYWQLLVLLLITAIG
                     AIIRFTHLAHPTLWNDEALVYWRTCGTYQQLLDVLRNDGFGPLHYELHWLIAQHFKPT
                     PEVLRSVPAICGTLMIPAIYFLARQMLPKSTSLVAAAFTACSAFLIFYSRDAKMYMDT
                     WLFAVLSVGALLWWLRSRRATAWLCWVAAGCTAVGIHASSMPVIALSPLFLLTHRSLR
                     WRQGLLMIAGLAVIFAGPLVYYTKFNRFIDNVEEDGWSRTGIQWVGIVNEGMTGPQLV
                     RNGATALGMGWAWPKDAAQELSSKPRWGWPRTNLVGPIAPAMLKWPKLAAELLISVLI
                     AAVLPWPRRERPRDHLHPLERGWRVLLWLSLWILLPGYIFYCHSVPRFTSPLTWPMEV
                     LPRTTGWLTDHSRFIEVLVAILLIAGAIWRPTRSALQKTIVIAVMFGVLYAIYSVCHE
                     QALKAAAEGRPWRSIWMPRYFGFLWPALGIATAALLMRLPTIYIRVAAVALLLGVNLA
                     FGAARVFAWTEPPVDQMAKDVYDAQPRNSATRTYTDVPRGDPNPGGGSIESIPGRYYL
                     QLYAWHQPMSPYRFRIQSIGEYVIRGGYRPMTIADEVRKNPQLKRVIVWEDNPFEAPH
                     DADELGPMLPGWRLASDTWYPVRTYWEWQERWNFRRVEYVR"
     gene            complement(5262..5699)
                     /locus_tag="VLI90_15590"
     CDS             complement(5262..5699)
                     /locus_tag="VLI90_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSV15684.1"
                     /translation="MFATFAPATLAQVPQTASGDPRYGWLPVLLLLIIGIGFAIGNIV
                     LSLIIGPSRTGPGKEATYESGMTPIGDTRKRFNVRFYVVAMIFLVFDVDIIFFYPLCS
                     IFPQTAIANHRLAGLLLVDIIVFVLVLLVAYLYAWGKGVFRWD"
     gene            5921..>6664
                     /locus_tag="VLI90_15595"
     CDS             5921..>6664
                     /locus_tag="VLI90_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15685.1"
                     /translation="MHFEDFKTWHWVIIGLLVGALFSGVKMYAGPWYTNADIDTLDQM
                     RFEHALIGQPDGGNRTNAQMLRYLVGKQMVKDVVVHPPTFGDTDGKYWVTGKIATMEP
                     MNQVPSDPKTAVVPAEIWRPFKYPADAPYQEIPPNVKRAPSRTALLAATRAATRPAAI
                     AAAAKKKKAATNPTTAAAAATATPAIPQIKIHHTVVEYLQSKQSDPNAHFSYRYAWQE
                     NKAAMAILPPIAGLLIIGIAWPVTLGLMQH"
BASE COUNT         1212 a         2157 c         2119 g         1176 t
ORIGIN      
        1 gtccagacct gctcgccact ggctacccca tttcgagctg ccgcccccgg cgcgcgcgta
       61 atcagcggtg ccgttgacgc ccggccacaa accggcgctg acaatgccga tcgccgcgcg
      121 cgatccgcgg atgcgctcgg tcgccgcctc caacgtcagg ttcgacgcca gcgcccgctc
      181 gaccagcgag ttcagcatcg gatcatcgaa cgtcgcccac caatgatcga ccgccaccgg
      241 cgcctgcacc ggtacgctgg cccgcgtggt aggcggcgac tcccaactcg ccggcatcgc
      301 ttgcgacggc ggatgataat ccggccccac cttgcaactg gcggccaaaa gcagcaagcc
      361 gacaaacgat ccgacggcaa caccgcagcg aatgaaccga gaaggagcca aagtcgagca
      421 gcctcaccgg cgggtgggaa atccaaagca catcctcgtg cggcgcggcc gcctcaaccc
      481 gcgcactgct agcaatgtaa cccaaccgcc cgtgcgatac ctagaccggc ggacttgtag
      541 cactggcgcc agagcccgtg ctacatcaga taccttcccc cactccccgc gtttgcagcc
      601 gaatcctgct tatctcccaa tctttttcag gaaccccgcc tcatccagga ccggcacgcc
      661 gagttccttc gccttatcca acttgcttcc cgcggactcg ccggccacga cgaagctcgt
      721 cttcttgctc acgctgccgc tcgcctttcc gccgagcttg aggatcagct cttcgatctc
      781 ctgccggccc agcgtcggca gcgtgccggt gacgacgacc gtctggccgg acagcggcag
      841 tgccgcgccg tcgcccgcgg gcttttccat ctttggatcc acgccaacgt ttttcagcgc
      901 cttcaccacg agcttgcccg catcgttgtg gaagtagtcg tgaacgctgt ccgcgatcac
      961 ctcgccgatc tcgtgcaccg cggccagctg ctcctgcgtc gcgccggcaa tcgcatcgag
     1021 cgaaccgaaa tggctcgcca acacgtgcgc cacgcggttg ccgacgtggc ggatgccgag
     1081 ccccgcgagc aggcggtcca gcggttggtt gcggctgttg tcgatgttct tgacgacctt
     1141 ctgcgcgacc ttgtcgccca ccgtgcgcgt caccgatttc ccgccctgct cgacttccga
     1201 agtgagtccg gcgaggtctt gcggcttgag cttgtacaag tcggcgaacg tgcgaacgag
     1261 cccgcgctcg accagctgat cgaccagcac atcgcccagc ccttcaatgt ccatctggct
     1321 gcgcccgcag aaatagcgga tcgcctcttt catccgcccc gggcacaggt cgttcgggca
     1381 gaggatgtcg atgccgctgt cgagcagttc gaccggcttc ccgcacaccg gacaatccgt
     1441 cggcaacttc gcgcgcttga cccttttccg ctcaaagaaa acatcgcacg cctcgttcac
     1501 gcagcggtac gccgcgtggc cttcggtcag cgcttcgcgc accaccggcg tcgcgcacgc
     1561 cggacatttc ttcggcggct gcacggactt ggcgccgctc ggccgcttgt ccttgtgcac
     1621 ttctacgacg tacggaatca cctcgccgct gcgctcgacg acgatcgtgt cgccgatgtg
     1681 gatgccgagt cgctggatct ggtcgatgtt gtgcagcgtg acgttggaga cggtcacgcc
     1741 gccgatgaag acaggctcga gcttgccgac cggcgtgagc tggcctgtgc gacccacctg
     1801 ccagtccacg tccttcagca ccgtgggctg ctgctcggtc tcgtatttgt atgcgatgac
     1861 ccagcgcggc gctttgctgg tcgcccccag ctgatcgcgg tgcgcgaacg cgtccacctt
     1921 catcaccatg ccatcggtca tgtacggcag cttgggccgc agcttttcga actggtgaat
     1981 gaacttcagc gccgcatcca cgccgggcac gtgtgagact tccttcggca gcgggaagcc
     2041 ccactgcccc agcatctgcg tccattccca atacgacttg gccggcatcg gttcgacctg
     2101 accgttgccg tgcgccagga agcgcagccg gcgcttggcg acgatcttgg ggtcgagccg
     2161 ccgcagcgtg ccggaggtga ggttgcgcgg gttggcgtac ggttcctcgc cttcggcttc
     2221 gatctcctta ttcactcgct gaaagtcgtc gttgtccatg tagacctcgc cgcgcacctc
     2281 cagaattttc ggcggcgtgt ccttgagcgt cagcgggatt gacttgatcg tgcgcgcgtt
     2341 gatcgtgacg tcatcgccga cgttcccgcg cccgcgcgtg gccgccagca cgagcttgcc
     2401 gccttcgtaa cgcaagctga tcgacgtgcc gtcgatcttc ggctccagca cgtagctggg
     2461 ctgctggccg tccagcgcct tgcgcacgcg ctcgtcgaac gcgcgcacct ccgcttcgct
     2521 gtacgtgttg tcgatgctca tcatcggcac ggcgtggcgg acgggcttca gctccgtctg
     2581 cacttccccg cccacgcgct gcgtcggtga atctggcgcg atcagatccg ggttggcgcg
     2641 ctccagatcg atcagctcct gcatcagccg gtcatattcg cggtcgctga ccgcgggccg
     2701 cgactgcacg taatagaggt agttgtgatg gttcagttcc tggcgcagct gctcgatccg
     2761 ctgcttcgcg tgggtcgcgg tgggcatgga agcattttaa gcgatcgggg gaatttcatc
     2821 actcgcgcgt cacgcgtcgt gcgcactgcg tagcacgggc gtctcgcccg tgatgcgtgt
     2881 actcatgcac ggcgatggta cgccatcgac acagcaatcg cgtcagcgac ggttgtcgat
     2941 gtcctgtcgc tgacgcgatc gcttcgccgg tggagtacca ccggcattcg tggggtacca
     3001 cgaaacacgg gcgagacgcc cgtgctacgg taccgctacg caacagagaa atttctcaac
     3061 gcacgtattc gacgcgccgg aagttccagc gttcttgcca ttcccaatac gtccgcacgg
     3121 gataccacgt gtcgctcgcc aggcgccaac ccggcagcat cggccccagt tcatccgcgt
     3181 cgtgcggcgc ttcgaacggg ttatcctccc acacgatcac gcgcttcagt tgcggatttt
     3241 ttcgaacttc atccgcgatc gtcatcgggc ggtatccgcc gcggatgaca tactcgccga
     3301 tcgattggat gcggaagcgg tatggcgaca tcggttgatg ccacgcgtac agctgcaggt
     3361 aatagcgtcc cgggatgctt tcgatcgacc caccgccggg gttggggtcg ccgcggggga
     3421 catcggtgta ggtgcgggtt gcgctgttgc gcggctgggc gtcgtagacg tccttggcca
     3481 tctgatcgac cggcggttcg gtccacgcga agacgcgggc ggcgccgaag gcgaggttca
     3541 cgccgagcag cagagcgacg gcggcgacgc ggatgtagat cgtcggcagc cgcatgagca
     3601 aagcggcggt cgcgatgccg agcgcgggcc agaggaagcc gaagtaccgc ggcatccaga
     3661 tcgaccgcca cggccggccc tcggccgcgg ctttgagggc ctgctcgtgg cagacgctgt
     3721 agatggcgta caacacgccg aacatcacgg cgatgacgat cgtcttctgc agggcgcttc
     3781 gcgtcggacg ccagatcgcg ccggcgatta agaggatcgc gacgagaacc tcgatgaagc
     3841 gcgagtggtc tgttagccat ccagtcgtgc gcggcaagac ttccatcggc cacgtcagtg
     3901 gcgaggtgaa tcgtggcacc gagtggcaat agaagatgta tcccggcagc aggatccaca
     3961 gcgacagcca gagcagcacg cgccatcccc gctccagcgg atgcaggtga tcgcgcgggc
     4021 gctcgcgccg cggccacggc aacaccgcgg cgatcagcac gctgatgagc aactccgccg
     4081 ccagcttcgg ccacttcagc atcgccggcg cgatcggccc gaccaggttc gtccgcggcc
     4141 agccccatcg cggcttgctc gacagctcct gcgccgcgtc cttcggccat gcccagccca
     4201 tccccagcgc cgttgccccg ttgcgcacca attgcggccc ggtcatcccc tcgttgacga
     4261 tgccgaccca ttggatgccg gtccggctcc agccatcttc ctcgacgttg tcgatgaagc
     4321 gattgaactt cgtgtaatac accagcggcc ccgcgaagat gaccgccaat cccgcgatca
     4381 tcaacagccc ctgccgccag cgcaacgaac ggtgcgtgag tagaaaaagc ggcgaaagcg
     4441 cgatgaccgg catcgagctg gcgtggatgc ccaccgcggt acaccccgcg gccacccagc
     4501 agagccacgc ggtggcccgg cgcgagcgca gccaccacag cagcgcgcca acgctgagca
     4561 ccgcgaagag ccaagtgtcc atgtacatct tggcgtcgcg cgaatagaag atcaggaacg
     4621 ccgagcacgc cgtgaacgcg gcggcgacaa gcgaggtgga cttggggagc atctgccggg
     4681 cgaggaaata tattgctggg atcatcagcg tcccgcagat ggcggggacg gatcgcagca
     4741 cctccggcgt cggtttgaag tgctgggcga tgagccaatg caattcgtaa tgcagcgggc
     4801 cgaacccgtc gttgcgaagg acgtcgagca gttgctggta ggtgccgcag gtgcgccagt
     4861 agacgagggc ctcatcgttc caaagcgtcg gatgggcaag gtgggtgaag cggatgatcg
     4921 cgccgatggc ggtgatcagc agcaggacga gcagctgcca atagcggcgg agcgccgatg
     4981 gggctgagcg ctgtgcgcga acgcgctggc gaacaacggg ggcgggggct tctaaatgag
     5041 ttaggctcag cattcaacgc tcaacatcta acggcgaagc gtgcgcgagt tggaccgcaa
     5101 aggcaacgcc gctacttcag aggtagcgtt ggatacgccc ggttgttgaa gattgaatag
     5161 accacttgcg aagcggtagc cccgcagggg cacgtgtttt tctctagaac ggaaatacgt
     5221 gcccctgcgg ggctaccgct acgcggggga gaacagtcgt cttaatccca ccggaacacg
     5281 cccttgcccc acgcgtacag gtacgcgacg agcaggacca ggacgaagac gatgatgtcc
     5341 accagcagca ggccagccag ccggtggttg gcgatcgcgg tctgcgggaa gatcgagcac
     5401 agcggataga agaagataat gtccacgtcg aagacgagga aaatcatcgc gacgacgtag
     5461 aagcgcacgt tgaagcgctt gcgggtgtcg ccgatcggcg tcatgccgct ttcgtaggtc
     5521 gcttccttgc ccgggccggt gcggctgggg ccgatgatca gcgacagcac gatattgcca
     5581 atggcgaagc caatgccgat gatgagcagc agcaataccg gcagccagcc gtaacgcggg
     5641 tcaccgctgg cggtctgagg cacttgggca agggtcgcgg gggcgaaggt cgcaaacatg
     5701 gtgagggcat aatctagaga cacgcgcggg aattgcaaac tcgacgttta ctccgtcatt
     5761 tagcgatcag ggatttctcc gcaatctggc aaccggttca ccgttaggtg gtagtaccct
     5821 tggtgagcca tatactggcg gcgtccggca aggatgcgcc gtttcgggca ttgtgggtga
     5881 aactttctag ccggcgaacg tctattaggc cgggggccgt atgcatttcg aagatttcaa
     5941 gacctggcat tgggtgatca tcgggctgct ggtcggcgcg ctgttcagcg gcgtgaagat
     6001 gtacgcgggg ccgtggtaca ccaacgccga catcgacacg ctcgaccaga tgcgctttga
     6061 gcatgcgctg atcgggcagc cggacggcgg caaccgcacc aacgcgcaga tgctgcgata
     6121 tctcgtcggc aaacagatgg tgaaggatgt ggtggtccat ccgccgactt ttggcgacac
     6181 cgatgggaag tactgggtga ccggcaagat cgctacgatg gagccgatga accaggtgcc
     6241 gagcgacccg aagaccgcgg tggtgccggc ggagatctgg cggccgttca aatatccggc
     6301 ggatgcgccg tatcaggaga tcccgccgaa cgtgaagcgg gcgccgagcc ggacggcgtt
     6361 gctggcggcg acgcgcgccg cgacacggcc ggccgccatc gcggcggcgg cgaagaaaaa
     6421 gaaggctgcg acgaatccga cgacggcggc ggcagcggcc acggcgacac cggcgatccc
     6481 gcagatcaag atccatcaca cggtcgtcga gtatctgcag tcgaagcaga gcgatccgaa
     6541 cgctcacttc agctatcgat atgcctggca ggagaacaag gcggcgatgg cgatcctgcc
     6601 gccgatcgcg gggctgttga tcatcggcat cgcctggccg gtgacgctgg gcttgatgca
     6661 gcac
//
LOCUS       DATIMH010000899         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1083631, whole genome shotgun sequence.
ACCESSION   DATIMH010000899 DATIMH010000000
VERSION     DATIMH010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083631"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VLI90_15600"
     CDS             <1..417
                     /locus_tag="VLI90_15600"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HSV15686.1"
                     /translation="PVRGDIPGRSNGVMISNVSGKANAFAMNTLQDRGVLFVEPNDEV
                     YEGQIVAENCRPDDIVVNPTKAKQLTNMRTTSSDENIILKPARKMTLEQALEYIEEDE
                     LVEVTPANIRLRKELLSENERKKKDRSSKKAVAATV"
     gene            556..1260
                     /locus_tag="VLI90_15605"
     CDS             556..1260
                     /locus_tag="VLI90_15605"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSV15687.1"
                     /translation="MRRPIIGITIDRCDNAERYESPFSYAKAIELAGGLPLLFPYKTD
                     LALIPQYVDLIDGMLFSGGNDLDPSLYGQTWHEKAERIDPDRQRWDMALMAEVERRRT
                     PTLGVCLGSQIMNVYRGGSLHQFLPDVPRDNSLEHRKVGTEIKRHPVTLDPASKIGRA
                     IGKPEISVNTYHKQSAQTLGRGLRVIATAPDGVIEGFEDPSFPLFAAVQWHPERLTDE
                     PEHLAPFKLLVEKAKK"
     gene            complement(1315..2583)
                     /gene="truD"
                     /locus_tag="VLI90_15610"
     CDS             complement(1315..2583)
                     /gene="truD"
                     /locus_tag="VLI90_15610"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HSV15688.1"
                     /translation="MLPYLTTDFPGVGGVIKQRPEDFFVQEMPLYEPSGEGEHVYCEI
                     QKTGITTFEAIHRLADALHVSSRDIGYAGMKDAHAITRQIFSIQGTTPEAVMSANLPG
                     IAVQWAARHGNKLRLGHLAGNRFAIKIREVNPTDVIKLKPVLDVIQRRGMPNFFGEQR
                     FGRRNNNDQLGAALVRGDNEAILKLLLGSPDPELDDGRTTEARRAFERRDNNEAMRLW
                     PRSGGMERRVLARLMKTHRPSAAVHAIDEKIRRLWVSALQSRAFNDVLAKRIETIDKL
                     MDGDLAYKQENGACFRVESAATEQPRCDAFEISPTGPLVGYRMTLPDGEPLRLEQEAL
                     AAVHLSPADFRQSGKLRVKGARRPLRVRPENMDLAAGVDEFGSHITVAFTLPSGAFAT
                     VLLRELMKENAPAAEVDEASIEASSEATAE"
     gene            complement(2770..>3785)
                     /locus_tag="VLI90_15615"
     CDS             complement(2770..>3785)
                     /locus_tag="VLI90_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15689.1"
                     /translation="RAKETTAPAAPLPRARVQQFAADVVLTPRLSVGTADPESIYEAK
                     FTGQIVASAADGERGDVELELPVPPQVISLADLTVSADNQPSDRVALRDGKLIWRGSL
                     NNTGPTALAVTYTAVGKGLYELSVPPGGILDQFKIALTANGSDVRMMELSLQPTSLAH
                     SPSSTTYTWDYKRLMFGQPIRLDVLGIAPIDRLGELTWLGPTSVVVFGLLLGMIVHAL
                     GIHTFDRWMLLLTLGTFAGAYPLMYFAQQFIHLQTAVLISASIALLIIAVRTITIMGF
                     LRAALGAVLPAAAIMAMTLVAALMPQLQGIILTAGAMAFFIAAMVLIPKLVVYPERGG
                     AAA"
BASE COUNT          700 a         1220 c         1226 g          639 t
ORIGIN      
        1 cccgtccgcg gcgacatccc cggccgcagc aacggcgtga tgatctccaa cgtcagcggc
       61 aaagcgaacg ccttcgcgat gaacacgctc caagaccgcg gcgtcctgtt cgtcgaaccg
      121 aacgacgaag tgtatgaagg ccagatcgtc gccgagaact gccgcccgga cgacatcgtc
      181 gtcaacccga ccaaggccaa gcagctgacg aacatgcgga cgacgtcgtc ggacgagaac
      241 atcatcctca agcccgcgcg gaagatgacg ctggagcagg cgctggagta catcgaggaa
      301 gacgagctgg tcgaagtgac cccggcgaac atccgcttgc gcaaggaact tctgagcgag
      361 aacgagcgca agaagaagga ccgcagttcc aagaaggccg tcgccgcgac ggtttaagat
      421 ctgaagctcg caaataaccc gccgcttgcg gcttagcgtc tgtttcgact ttgggacaga
      481 aacaaacgct aagccgcaag cggcgttttg ctgcgctctt gcgccaccga aaactcctgc
      541 tatcatccgg caatcatgcg acgccccatc atcggcatca ccatcgaccg ctgcgacaac
      601 gccgagcgct acgagtcgcc gttctcgtat gccaaggcca tcgaactcgc cggtggtttg
      661 ccgctgctgt tcccgtacaa gacggacctg gcgctgatcc cgcagtacgt cgatttgatc
      721 gacggcatgc tcttcagcgg cgggaatgat cttgatccat cactctacgg ccagacgtgg
      781 cacgagaagg cggagcgtat cgaccccgac cgccagcgct gggacatggc cctgatggcc
      841 gaagtcgaac gccgccgcac gccgacgctg ggcgtctgcc tgggctcaca aatcatgaac
      901 gtctaccgcg gcgggagcct gcaccagttc ctgccggacg tgccgcgcga caactcgctc
      961 gaacaccgca aggtggggac ggaaatcaaa cgacacccgg tgacgctcga cccggcgagt
     1021 aagatcggcc gtgcgatcgg caagccggaa atctccgtca acacgtatca caagcaatcc
     1081 gcgcaaacgc taggccgcgg cctgcgcgtg atcgcgacgg cgcccgatgg cgtgatcgaa
     1141 ggattcgaag acccgtcgtt cccgctcttc gccgccgtgc aatggcaccc cgagcggctg
     1201 acggatgaac cggagcacct ggctccgttc aagctgctgg tcgaaaaggc gaagaagtga
     1261 tggtccgtac cccctctccc acgagtacgt gggcgagggg accggatgga gccgtcactc
     1321 cgccgtcgcc tcgcttgacg cttcgatcga agcttcatca acttcagccg cgggcgcatt
     1381 ctctttcatc aactcccgca gcagcaccgt cgcgaaagcc ccggaaggca gcgtgaacgc
     1441 gaccgtgatg tgggagccaa attcatcgac cccagcggcg aggtccatgt tctccggccg
     1501 cacgcgcagc ggtcggcggg cgcccttcac gcgaagcttg ccgctttggc ggaagtcggc
     1561 gggcgacaga tgcaccgcag ccaaagcttc ctgttccaac cgcagcggct cgccgtcggg
     1621 aagcgtcatg cgataaccaa ctaacgggcc ggtgggactg atctcgaacg catcgcatcg
     1681 cggctgctcg gtcgcggcac tttcgacgcg gaagcaggcg ccgttctcct gcttgtacgc
     1741 caggtcgccg tccatcagct tgtcgatcgt ttcgattcgc ttggcgagca cgtcattgaa
     1801 cgcgcgcgat tgcagcgccg acacccacag ccggcggatc ttttcgtcga tggcatgcac
     1861 cgccgcggag ggacggtggg ttttcatgag ccgcgcgagc acccggcgtt ccatgccgcc
     1921 ggagcgcggc cacagccgca tcgcttcgtt gttgtcgcga cgctcgaatg cgcgccgcgc
     1981 ttcggtcgtg cggccatcgt ccagttccgg gtcgggcgat ccgagcagca gcttcaggat
     2041 cgcttcgttg tcgccgcgga cgagggccgc gccaagttgg tcgttattgt tccgccggcc
     2101 gaagcgctgt tcgccgaaaa agttcggcat cccgcggcgc tggatcacgt cgagcacggg
     2161 cttgagcttg atgacatccg tcgggttcac ctcgcggatc ttgatcgcga agcgattgcc
     2221 ggccagatgt cccagccgca gcttgttgcc atgacgggcc gcccactgca cggctatgcc
     2281 gggcagattc gcgctcatca ccgcttcggg cgtggtgcct tggatcgaga agatctgccg
     2341 cgtgatcgcg tgcgcatcct tcattccggc gtagccaata tcgcgcgacg agacatgcag
     2401 tgcatcagcc aggcggtgga tcgcttcgaa cgtggtgatg ccggtcttct ggatttcgca
     2461 gtagacgtgc tcgccttcgc cgctgggctc gtagagcggc atttcctgga caaagaagtc
     2521 ttcggggcgc tgtttgatca cgccaccaac gccggggaaa tctgtggtga gataggggag
     2581 catgtcggcc gtcatcctga gcgaagcacg tactcgtgcg cagtcgaagg atctggccga
     2641 aacgtcggtg aaccctcttt gcgcgcgcga tcgttcgcag accatgccac ggcttctgcg
     2701 aaaccgcaag cgactgcggt gccgatttgg cgagctgcgc gtcgccggct tccaggccag
     2761 atccttcgac tacgcggctg cgccgccgcg ctcaggataa acgacaagct tcggaatcaa
     2821 caccatcgcc gcgatgaaga acgccatcgc gccggcggtc aggatgatcc cctgcagctg
     2881 cggcatcagc gcggcgacca acgtcatcgc catgatcgcc gccgcgggca gcacggcgcc
     2941 gagcgccgcc cgcaagaagc ccataatcgt gatcgtgcgc accgcgatga tcagcagcgc
     3001 gatgctggcg gagatcagca ccgcggtctg caggtggatg aactgctgcg cgaagtacat
     3061 cagcgggtac gctcctgcga acgtgccgag cgtgagcagg agcatccagc ggtcgaacgt
     3121 gtggatgccc agcgcgtgca cgatcatgcc caggagcagc ccgaagacga cgacgctggt
     3181 ggggccgagc catgtgagtt cgcccaggcg gtcgatcggc gcgatgccga gcacgtcgag
     3241 ccggatcggc tggccgaaca tcaggcgttt gtagtcccac gtgtacgtcg tcgagctggg
     3301 cgaatgggcc aggctggtcg gctgcagcga cagttccatc atccgcacgt ccgagccgtt
     3361 ggcggtgagc gcgatcttga actgatcgag aattccgccg ggcgggacgg agagctcgta
     3421 caggcccttg ccaaccgcgg tgtacgtgac ggcgagcgcg gtgggaccgg tgttgttgag
     3481 ggagccgcgc cagatgagct tcccgtcgcg cagcgcgacg cggtcgctgg gttggttgtc
     3541 ggcgctgacg gtgaggtccg ccagcgagat cacctgcggc gggaccggca gttccagctc
     3601 gacgtcgccg cgctcgccat cggccgcgct ggcgacgatc tggccggtga acttcgcttc
     3661 gtagatcgat tccgggtcgg cggtgccgac gctgagccgc ggcgtgagca ccacgtcggc
     3721 ggcgaactgc tgcacccgcg cccgcggcag cggagcggcg ggggcggtgg tctctttggc
     3781 acgca
//
LOCUS       DATIMH010000900         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1375341, whole genome shotgun sequence.
ACCESSION   DATIMH010000900 DATIMH010000000
VERSION     DATIMH010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375341"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VLI90_15620"
     CDS             complement(<1..503)
                     /locus_tag="VLI90_15620"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSV15690.1"
                     /translation="MPKPYKFRYVNQIAGAFAGFILLILVVGLVLVARAQKWFEPVRH
                     VTVRMPSEGCYGLKAGGDVRIMGAVVGAIDSIEIQPKTGLMVASVHVRPDFAEFIRLG
                     TADVKAIIHVPIGFADPYLEILNAKAFATEVTPKGLVVAEHEADASEAFNRIGKYVDT
                     VGPTVDKL"
     gene            complement(510..1247)
                     /locus_tag="VLI90_15625"
     CDS             complement(510..1247)
                     /locus_tag="VLI90_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15691.1"
                     /translation="MKNDDRTILAFDAVTTAADVLYDARIIGATFALRAGELALIRFD
                     PHAPRSPLPDAAMGLAELSGGAVQILGQSWEALSPRASCRLRGRIGRLFGMQDAWVDH
                     LDIDENVTLRLRHHTRRRLSVIDREANELSRAFGLPGLPTIRPATAEPRDLQRAACAR
                     MLMGSPQLLIIDEPASGLYSDVLAALMREVAVARRRHVAVLWISADPIVWTDDSIAPT
                     FRGDAGEPGLALMDLAKTATLAGLTAG"
     gene            complement(1249..>1697)
                     /locus_tag="VLI90_15630"
     CDS             complement(1249..>1697)
                     /locus_tag="VLI90_15630"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV15692.1"
                     /translation="SASAVASEMANMQLAGEVRLLDAQGLDPLVYLVMPRVVGLSVCA
                     FGLSILFVVVSLVSGYLFVTLLGHAIRPRDFADSVFGALSPVDMLNLLGKSVIPAALT
                     AVICCTEGLSVGAGGTEIPRATSRGLARSVVAMFVVAGAISVLTYL"
BASE COUNT          322 a          573 c          539 g          263 t
ORIGIN      
        1 agcttgtcca cggtcggccc gaccgtatcg acatacttgc cgatgcgatt gaatgcctcg
       61 ctggcgtcgg cctcgtgctc ggcgacgacc agccccttag gcgtgacttc agtcgcgaac
      121 gccttggcgt tcaggatttc cagataaggg tcggcaaagc cgatcggcac gtgaatgatc
      181 gccttcacat cggcggtgcc gaggcggatg aattcggcga agtccgggcg cacgtgcacc
      241 gatgccacca tcaaccccgt tttgggttga atctcgatgc tgtcgatcgc gccgacgacc
      301 gcgcccatga tgcgcacgtc gccgccggcc ttcaggccat aacatccttc ggacggcatg
      361 cggaccgtca cgtgccgaac cggctcaaac catttctgcg ctcgcgcgac caacaccagc
      421 ccgaccacga ggatcagcaa aatgaacccg gcgaacgccc cggcgatctg gttcacgtag
      481 cggaatttgt agggcttggg catatcgcgt tatcccgcgg ttagaccggc cagagttgcg
      541 gttttggcca gatccatcaa tgccaggccg ggttcaccgg cgtcgccgcg gaacgtgggg
      601 gcgatgctgt cgtcggtcca gacgatcggg tcggcagaga tccagagcac ggcgacatga
      661 cgacggcgcg cgaccgcaac ctcgcgcatc agcgcggcca gcacatcgga atagaggcca
      721 ctggccggtt cgtcgatgat cagcagctgt ggtgaaccca tcagcatgcg ggcgcacgcg
      781 gcccgttgca gatcgcgcgg ctccgccgtc gccggacgga tcgtgggcag gcccggcaaa
      841 ccgaaggcgc gggagagttc gttcgcctcg cggtcgatga cgctgagtcg ccggcgggtg
      901 tgatggcgca ggcgcagcgt gacgttttcg tcgatgtcga ggtgatcgac ccacgcatcc
      961 tgcatgccga agagccggcc gatgcggccg cgcaaccggc aactcgcccg cggcgacagc
     1021 gcctcccacg actgaccgag gatctgcacc gccccaccgc tcagttccgc caggcccatg
     1081 gccgcgtccg gcagcggtga ccgcggcgcg tgcgggtcga agcgaataag cgctaattca
     1141 ccggcgcgca gcgcaaaggt cgcgccgatg atccgcgcgt cgtagagcac atcggcggcc
     1201 gtcgtcacgg cgtcgaacgc gaggatggtg cgatcgtcgt ttttcatctc acagataggt
     1261 caaaaccgaa atcgcgccgg ccaccacgaa catcgcgaca acggatcgcg ccagtccccg
     1321 gctggtcgcg cgcgggattt ccgtgccgcc cgcgccgacg ctcaatcctt ccgtgcagca
     1381 gatgaccgcc gtcaacgccg cggggatgac gctcttgccg agcaggttca gcatgtccac
     1441 cgggctcagc gcaccgaaga cgctgtcagc gaaatcccgc gggcgaatcg cgtggcccag
     1501 cagcgtcacg aacaggtaac cactcaccag cgagacgacg acgaatagga tgctcaaccc
     1561 gaacgcgcac accgaaaggc cgacgacgcg cggcatcacc aggtagacca gcgggtccag
     1621 cccctgtgca tcgagcaatc gcacttcgcc ggccagctgc atgttcgcca tctccgatgc
     1681 aacagcgctg gcggatc
//
LOCUS       DATIMH010000901         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3682618, whole genome shotgun sequence.
ACCESSION   DATIMH010000901 DATIMH010000000
VERSION     DATIMH010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3682618"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VLI90_15635"
     CDS             complement(<1..470)
                     /locus_tag="VLI90_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HSV15693.1"
                     /translation="MKCDSCNKPATVHLTEIKNGKKIEKHLCEQCAAQSEGLSTKSHM
                     PINELLTNFVMAHSGLQSKQQEGLVCDQCGVTWSDFRQSGLLGCEHDYTIFEKEMTPL
                     LQRAHEGAAHHVGKVPTRRGGTGVPVKKTADLTKLRKELARAVETEDYERAAKLR"
     gene            complement(632..994)
                     /locus_tag="VLI90_15640"
     CDS             complement(632..994)
                     /locus_tag="VLI90_15640"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSV15694.1"
                     /translation="MPFEIRTVRSFSAAHQLRLYDGSIEPLHGHNWRVQVTVSAPQLD
                     AIGVVMDFHELERLIDQIITPMHNRHLNDLPAFAQVNPSAENVALHIGTTLRLPANAR
                     LQRVQVWETPENSAVYRP"
     gene            1074..1766
                     /locus_tag="VLI90_15645"
     CDS             1074..1766
                     /locus_tag="VLI90_15645"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV15695.1"
                     /translation="MTFATLPNPPDAPTEVETLADLLEQLGVPPERVWLRPAPGTATE
                     ADIIYADDHLDRLCELIDGTMVEKAMGTRESLIATVISSLLYAFVLPRKLGFVVGADS
                     MFRLFPGQVRMPDVAFISLKRLPSGIATEPIAPIAPELAVEVLSASNTPQEMKRKRRE
                     YFNSGVQLLWLVDPNLRTIDVYTAPENPVRRQVTDALDGGVVLPGFSVPVADLFSMLD
                     LRTDDQSQGSAL"
     gene            1800..3299
                     /gene="trpE"
                     /locus_tag="VLI90_15650"
     CDS             1800..3299
                     /gene="trpE"
                     /locus_tag="VLI90_15650"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HSV15696.1"
                     /translation="MRRYFPDISAFTTAASAADIVPVYRQLLADHLTPVTAFEVLGRD
                     GNAFLLESVVGGEKIARYSFIATNPSLLYQAGGANAIIQQKGKPPKEFETTDPLADLQ
                     ALLPERRYHRAKDLPTFTGGLVGYAGYDTIRYYEGEKLPNPPKDDRRLPDVLFGLYGE
                     TVVFDHVDKTIKVIANADIKAASGDVDAAYKDACRRIDELVSRLQQPPTSKLGEIDPR
                     GPVNLPFASNMTREQYEEAVRKGQEYIKAGDIFQFVPSQRVRVHSTADPFDVYRALRI
                     INPSPFMFFLKSPVCTLIGSSPEILCRVTDRIATSRPLAGTRKRGATEEEDKALEQEL
                     LADPKERAEHIMLVDLHRNDIGRVAKIGSVVVSDCMIVERYSHVMHITTNVTGVLEDD
                     KTALDALKVSLPVGTVSGAPKIRAMQIIDELEPTRRGPYGGAVGYLDYAGNLDTCIAL
                     RTIVWRNGVYDIQVGAGVVADSIPSNEYDETMSKAKAMLKAVEIAQNGF"
     gene            3418..4209
                     /locus_tag="VLI90_15655"
     CDS             3418..4209
                     /locus_tag="VLI90_15655"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSV15697.1"
                     /translation="MSGDAHVELLFLGTGTSAGIPMIGCHCEVCSSANPRDKRTRPSV
                     VISQGQTRVLVDTSPELRLQCVANGVDMIDALVITHAHADHIMGLDDVRRFNALRNGP
                     LDVWAEPHTHDVVGRCFGYAFQPPSPDLKVFRPHLQRREIRGDFHIGPMSWRPIRLFH
                     GEQPILGFRVGRLAYCTDVNRIPPESWGLLQDLDVLVLDALQWKPHTTHFSVDQALEV
                     VERVKPQQARFTHIAHALGHEATNAKLPANVRLAYDGERITARIA"
BASE COUNT          803 a         1346 c         1272 g          810 t
ORIGIN      
        1 cgcagcttcg ctgctcgttc gtaatcttcc gtctccaccg cgcgggccag ctccttgcga
       61 agtttggtca ggtcggccgt cttcttcacc ggcacccccg tgccgccgcg gcgggtcggc
      121 accttgccga catgatgggc ggcgccttca tgcgcccgct gcagcagcgg ggtcatctct
      181 ttttcaaaga tcgtgtaatc gtgctcgcaa ccgagcaggc cgctctggcg gaagtcggac
      241 caggtgacgc cgcactggtc gcaaaccagc ccttcctgtt gtttgctctg caggccgctg
      301 tgggccatca cgaagttggt cagcagctcg ttgatgggca tgtgcgactt ggtgctcaac
      361 ccttcgctct gggcggcaca ctgctcgcac aaatgcttct cgatcttctt gccgttcttg
      421 atttccgtca ggtgaacggt cgccggttta ttgcagctat cgcacttcat atctgcatcc
      481 ttggaggccg gcggccaatg tcaaacgacg aacctaatcc gctccaaaca atcgtatttc
      541 cgctgcgcgc tagcgtcaac gtgttcccga aggttgcacg ggcccgtcgc gcgtgcgcgt
      601 gcgtctcgga catatatcgg atacttgacc ctcaaggtcg ataaaccgcc gagttttccg
      661 gcgtctccca cacctgcacg cgctgcagcc gagcattcgc cggcaatcgc agcgtcgttc
      721 caatatgcag agcgacgttt tccgccgacg gattgacctg cgcgaacgcc ggcaggtcgt
      781 tcaggtgccg gttgtgcatc ggcgtgatga tctggtcgat caagcgttcg agttcgtgga
      841 aatccatcac aacgccgatg gcgtccaatt gcggcgccga cacagtgact tgcacccgcc
      901 agttatgccc atgcagcggc tcgatcgagc cgtcgtaaag tcgcagctga tgagccgcgg
      961 agaaggagcg cacggtgcgg atttcgaagg gcatagctag atggtcgcga agggacaaag
     1021 gttcgcaagc atcgatcgta ttcacgaaca tgctatactt gcggaggata gccatgacgt
     1081 ttgcgacact tccaaatcct cctgacgcac cgaccgaggt ggaaacgctc gctgacctgc
     1141 tcgagcagct tggcgttccg ccggagcggg tttggttgcg gcctgcgcca ggcactgcca
     1201 ccgaggcgga cattatctac gcggatgacc atctcgatcg attgtgtgag ctcatcgatg
     1261 gcacgatggt ggagaaggca atggggacgc gcgagtcgct tatcgcaact gtcatcagtt
     1321 ctctactcta cgctttcgtg cttccgcgta agttagggtt tgtcgtcggt gcggacagca
     1381 tgttccggct attccccggc caggtccgaa tgccagatgt ggcctttatt tcgttgaagc
     1441 gactgccgag cggaatcgct accgagccga tcgcgccgat tgctccggag ttagcggtag
     1501 aagtcttgag cgccagcaac acacctcagg aaatgaaacg aaagcggcgg gagtatttta
     1561 actcgggagt gcaattgttg tggcttgtcg atccgaatct tcgaaccatt gacgtctaca
     1621 ctgcccccga aaacccagtg cggcgtcagg tcacggacgc gcttgatggt ggcgttgttc
     1681 ttcctggctt cagcgtgccg gtcgctgatc tgttttctat gcttgatctt cgcacagacg
     1741 accagagcca aggctccgcc ctgtgacaaa cagttcggcc cccctacaat gccgccatca
     1801 tgcggcggta tttccctgac atcagcgcct tcaccaccgc ggccagcgcc gctgacattg
     1861 tgccggtcta tcggcaactg ctcgccgacc acctgacacc ggtgaccgcc ttcgaagtcc
     1921 tcggccgcga tggcaatgca ttccttctgg aatccgtcgt cggtggtgag aagatcgcgc
     1981 gctacagctt catcgcgacc aatccgtcgc ttctctatca agccggcggg gccaacgcga
     2041 tcatccagca gaagggcaag ccgccgaagg aatttgagac caccgatccg ctggcggacc
     2101 tgcaggcgct gctgcccgag cgccgctatc atcgcgccaa agatttgccg acgttcaccg
     2161 gcgggcttgt tggctatgcg gggtatgaca ccatccgcta ttacgaaggc gaaaagctgc
     2221 cgaacccgcc gaaggatgac cggcgattgc cggatgtgct cttcggcctc tatggcgaaa
     2281 ccgtcgtctt cgatcacgtc gataaaacga tcaaggtcat cgccaacgcc gacatcaaag
     2341 ccgccagtgg ggatgtggat gccgcgtaca aagacgcgtg ccggcggatc gacgaactcg
     2401 tctcgcggct tcaacaaccg ccgacgagca agctgggcga gatcgacccg cgcgggccgg
     2461 tgaacctgcc gttcgccagc aacatgacgc gcgagcagta cgaagaggcg gtgcgaaaag
     2521 ggcaggagta catcaaggcc ggcgacatct ttcaattcgt gcccagccag cgcgtgcgcg
     2581 tgcacagcac cgccgatccg ttcgacgtgt atcgcgcgct gcggatcatc aacccgtcgc
     2641 cgttcatgtt cttcctgaag agccccgttt gcacgctgat cggctccagc ccggaaatct
     2701 tgtgccgcgt gaccgatcgc attgccacaa gccgcccgtt ggccggcacg cgcaagcgcg
     2761 gcgcgacgga agaggaagac aaggcgctgg agcaggagtt gctggccgat ccgaaggagc
     2821 gggccgagca tatcatgctc gttgacctgc accgcaacga catcggccgg gttgcgaaga
     2881 tcggttccgt cgtcgtcagc gactgcatga tcgtcgagcg ctacagccac gtcatgcaca
     2941 tcacgaccaa cgtcaccggc gtgctggaag acgacaagac cgcgctggac gcgctgaagg
     3001 tgagcctgcc ggtcggcacg gtcagcggcg cgcccaagat tcgcgcaatg cagatcatcg
     3061 atgaactgga gccgacccgc cgcgggccgt atggcggcgc cgtcggttac ctcgactacg
     3121 ccggcaatct cgacacctgc atcgccctgc gcacgatcgt ctggcgcaac ggcgtctacg
     3181 acatccaggt cggcgccggt gtcgtcgccg actccatccc gtccaacgaa tacgacgaaa
     3241 caatgagcaa agccaaggcg atgctcaagg ccgtcgagat cgcgcagaac ggtttctaac
     3301 gcacaacgcg cggtcgtgta gccggcgagc ttgctcgccg tatttcgcgc ccgaacggcg
     3361 agcaccctcc ccggctacac gttccgattc cccatcccct cgactataac caacgccatg
     3421 agtggcgacg cgcacgtcga acttctgttc ctcggcaccg gcaccagtgc cggcattccg
     3481 atgatcggct gccattgcga agtgtgcagc agcgcgaacc cgcgcgacaa gcgcacccgg
     3541 ccttcggtcg tcatctcaca aggtcagacg cgcgtgctcg tcgacacctc gccggaactg
     3601 cggctgcagt gtgtcgccaa cggcgtggac atgatcgacg cgctggtgat cacgcacgca
     3661 catgcggatc acatcatggg cctggatgac gtccggcgat tcaatgcgct gcggaacgga
     3721 ccgctcgacg tgtgggccga gccgcacacg cacgatgtcg tcggccgatg tttcggctac
     3781 gcttttcaac cgccgtcgcc ggacctgaag gtctttcgcc cgcacctgca gcggcgggag
     3841 atccgcggcg attttcacat cggcccgatg agctggcggc cgatccgcct gttccacggc
     3901 gagcagccga tcctcggctt ccgcgtcggc cggttggcgt actgcacgga cgtcaaccgc
     3961 atcccgccgg agtcgtgggg attgctgcaa gacctggacg tgctcgtgct cgacgcgttg
     4021 cagtggaagc cgcacacgac gcatttttcg gtggatcagg cgctggaggt ggtcgagcgc
     4081 gtgaagccac agcaggcgcg cttcacgcac atcgcacatg cgctgggcca cgaagcgacc
     4141 aacgccaagc tcccggcgaa cgtgcgcctg gcgtacgatg gggaacgcat caccgcgcgg
     4201 attgcataat ccacttgata gccgggggcg g
//
LOCUS       DATIMH010000902         3707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1662869, whole genome shotgun sequence.
ACCESSION   DATIMH010000902 DATIMH010000000
VERSION     DATIMH010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662869"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1723)
                     /locus_tag="VLI90_15660"
     CDS             complement(125..1723)
                     /locus_tag="VLI90_15660"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSV15698.1"
                     /translation="METRNQNVGEAPIAPSVELLESRTLLSTYYVSSGGNDGAAGTST
                     GAAWRTIARVNAASLKPGDRVLFAGGQTFSGGINLKNGGSSSSPIVIGSYGSGHAIIK
                     SGTRDGAYLLNKGGVWFQNLTFVGSPNGWQHDGIHFEIVSGRMSNIRVENCTISGYGG
                     NGVFVMGDRSGAGFSDVRITGNTVHDNVNTGIATFALSHNAISGVYIANNDVYDNYGD
                     GKSTCTGSGIMLGGLNGAVVEHNVAHHNGYKGGNGNLGIWAYDSNNVILQYNESYGQH
                     SFRGQDGGGFDFDADTSNSIMQYNYSHDNDGNGFQLNQWRNNSLFTGDIVRYNVSQND
                     ARKNNYAGLEAWGKVLNSTFYNNVSFVSPSKAGGNPSGIKVHNASVGGLYVSGLHFAN
                     NIVITTGGRPLVLVSSAELGGAKDLRFVGNDYWSSGSAANFNFGRTYTSLSAWQSATG
                     QEKLNGKSVGLQVDPKLTAAGTGGVLNGNLAQLSAYRLQSTSPLINKGVNVATVFGVA
                     GVSKDFFGDPIAAGAASEIGVDQV"
     gene            2166..>3707
                     /locus_tag="VLI90_15665"
     CDS             2166..>3707
                     /locus_tag="VLI90_15665"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HSV15699.1"
                     /translation="MVAILFAGAAFWWPIHARAVSPDAAVETAAVESHGGFTPFSLFH
                     DPVYTTFERTALIVVLFIAVAGLAYAGMLIRQVNKADRGTPKMQAIAAAVREGANAYL
                     GRQFLKIAPLIVAITALLYFTSSNEIAFRIGRAGAFLVGSLFSFCVGFVGMRLATTGN
                     LRVAAAARRSYGEAMQLGYRTGTVTGMLTDGLGLLGGTLIFLVFSERAYEALLGFGFG
                     GTLIALFMRIGGGIYTKAADVGADLVGKIEKNIPEDDPRNAATIADNVGDNVGDCAGM
                     SADIFESYEVTIVAAMILGLASFGHIGVVFPLLVRGIGVLGSIISTYTVKASKDDTSD
                     SALNSVHRGFWIGSAISVVGFFVLGFFYLRFNTSYLGNYAQAAAGFPGGDPKALPFFA
                     NLGFPGLDLRPAITCLIGVILAIVLNKQTSYFTHTSHGPVKALARSCRSGHATNIIEG
                     FAVGYESTVAAVIAVGAAILLSVLCYAGTPPIFVCFGVAMTGIGMLTLTGNTISMDVF
                     GPVADN"
BASE COUNT          648 a         1175 c         1120 g          764 t
ORIGIN      
        1 gtccgcggac gcggccaaga ctggcaaata catcgagccg catcctgaac tgacttttca
       61 aaagttcgtt tcgccgttgc tggcgcacgt ggcgtcgatc tgcaactaga cgaatacgcg
      121 atcgctacac ctgatccacc ccaatctccg atgcggcgcc ggcggcgatg gggtcgccga
      181 agaaatcttt gctcaccccg gcaacgccga agacggtggc gacgtttacg cctttgttga
      241 tgagcggcga tgtcgactgc agccgatacg cgctgagctg cgccagattg ccattcaaca
      301 ctccgccggt gccggcggcg gttagcttgg ggtcgacctg caagcccacg ctcttgccgt
      361 tcagcttttc ctgtcccgtg gcggattgcc aggcgctgag gctcgtgtag gttcggccga
      421 agttgaagtt ggcggcgctg ccgctggacc agtagtcgtt gccgacgaag cggaggtctt
      481 tagcgccgcc caattccgcg gagctgacca gcaccagcgg ccggccgccg gtggtgatga
      541 cgatgttgtt cgcgaagtgc agtccgctga cgtacaggcc accgaccgac gcgttgtgca
      601 ccttgatgcc cgacgggttg ccgccggcct ttgacggcga aacgaacgac acgttgttgt
      661 agaacgtgct gttcagcacc ttgccccacg cctccaggcc agcgtagtta tttttccgcg
      721 catcgttttg gctgacgttg tagcggacga tatcgccggt gaagagcgaa ttgttccgcc
      781 actggttcag ctgaaaaccg ttgccgtcgt tatcgtgcga gtagttgtac tgcatgatgg
      841 aattcgacgt gtcggcgtcg aagtcgaacc ccccgccgtc ctgtccgcgg aagctgtgct
      901 ggccgtacga ctcgttgtat tgaaggatga cgttgtttga atcgtacgcc cagatgccga
      961 gattgccgtt cccgcctttg tagccgttgt ggtgcgcgac gttgtgctcg accaccgcgc
     1021 cgttcaatcc gccgagcatg atcccgctgc cggtgcaggt gctcttgccg tcgccatagt
     1081 tgtcatagac atcattgttc gcgatgtaca cgccgctgat tgcattgtgg ctgagcgcga
     1141 aggtggcgat gccggtgttg acgttgtcgt gcacggtgtt gccggtgatg cgcacgtcgc
     1201 taaagccggc gccgctgcgg tcgcccatga cgaagacgcc gttcccgccg tatccgctga
     1261 tcgtgcagtt ctcgacgcgg atgttcgaca tccgcccgga gacgatctcg aagtggatgc
     1321 catcgtgctg ccagccgttg ggtgacccga cgaaggtcag gttctgaaac cacacgccgc
     1381 ccttgttcag caggtaagcg ccgtcgcgcg tgccggactt gatgatggcg tgaccgctgc
     1441 cgtaggaacc gatgacgatg ggtgatgagc tggacccgcc gttcttcaaa ttgatcccgc
     1501 cgctgaacgt ctgtccacca gcgaagagca cgcgatcgcc cggcttcaaa ctggcggcgt
     1561 tgacgcgcgc gattgttcgc caagccgccc ccgtcgatgt gccggcagca ccatcgttcc
     1621 cgccgctcga cacgtagtac gtggacagca gcgtgcgcga ctccagcagt tccaccgaag
     1681 gcgcgatcgg cgcctcaccg acattttgat ttctcgtttc catagcctac gacgcaatat
     1741 tcaaagctgc ggggtgtctg caaccgaaca tcgagcagaa aaaaagaaaa ttgcgcggca
     1801 ccgcagacat gaccgtgtcg acggccgtaa gaggatttac ctatccgctt acaagggcct
     1861 cttccgacgc tttatttgga tgtttccagc cacgctcggc cattgaccgg gtcgataaca
     1921 agactgtacg atcctttgag catcagccgc tccgccccgg cgggtgctgg tgaagcctcg
     1981 gtgactcgcg tgcaaccgcc cccgcgcagt gtgtgaacgc acgtgtgata gccaaggccc
     2041 cacccagtag cggcgaacga cgatggagtt cggcaatgtc ccgcggcaac ccctctaacg
     2101 gcagcagcaa gagcagcagc agcagcaagc gacgctcgag tcctgctcgt atcggaatat
     2161 tggcattggt cgcaatcctt ttcgccggcg ccgccttttg gtggcccatt cacgcccgcg
     2221 ccgtcagccc cgacgcggcg gtcgaaacag cggcggtgga atcgcacggc ggtttcacgc
     2281 ccttctcgct ctttcatgat cccgtttaca caaccttcga acgcacggcg ctgatcgtcg
     2341 tgctcttcat cgccgtcgcg ggcctggcct acgccgggat gctcatccgc caagtgaaca
     2401 aggccgaccg cggcacaccg aagatgcagg ccatcgccgc cgccgttcgc gaaggcgcga
     2461 acgcgtatct cggccggcag ttcctgaaga tcgcgccgct gatcgtcgcg atcaccgcgc
     2521 tgctgtactt cacctcgagc aatgaaatcg cgttccgaat cggtcgagct ggagcgttcc
     2581 tggtgggttc actcttcagc ttctgcgtcg ggttcgttgg gatgcggttg gcgaccactg
     2641 gcaaccttcg cgtcgccgcc gcagcccgac gcagctatgg cgaagcgatg cagctgggct
     2701 atcgcaccgg caccgtgacc ggcatgttga ccgacgggct tggcctgctc ggtggcacgc
     2761 tcatcttcct ggtcttctcc gaacgcgcgt atgaagcgct gctgggcttc ggtttcggcg
     2821 gcacgctgat cgcgctgttc atgcgaatcg gcggcggcat ctacaccaag gcggccgacg
     2881 tcggcgccga cctggtcggc aagatcgaaa agaacattcc ggaagacgac ccgcgcaacg
     2941 ccgcgacgat cgccgacaac gtcggtgata acgtcggcga ctgtgccggt atgtcggccg
     3001 acattttcga aagttacgaa gtcacgatcg tcgccgcgat gatcctgggg ctggccagct
     3061 tcgggcacat cggcgtggtc ttcccactgt tggtccgcgg catcggtgtg ctcggatcga
     3121 tcatcagcac ctacaccgtc aaggccagca aggacgacac gtccgatagc gccttgaaca
     3181 gcgtgcaccg cgggttctgg atcggctcgg ccatctcggt ggtcggtttc ttcgtcctcg
     3241 gcttcttcta tctgcggttc aatacctcgt atctcggcaa ttacgcccag gccgcggcgg
     3301 gtttccccgg tggtgatccc aaagcgctgc cgttcttcgc caacctcggc ttcccgggac
     3361 tcgatcttcg accggctatc acctgcctga tcggtgtgat cctggcgatc gttttgaaca
     3421 agcagaccag ttacttcacg cacacgtcac atgggccggt gaaagcgctg gcccgcagct
     3481 gccgcagcgg acatgccacc aacatcatcg aagggttcgc ggttggatac gaaagcacgg
     3541 tcgccgcggt gatcgccgtc ggcgccgcca tcctgctgtc ggtgctctgc tacgccggca
     3601 cgccgcccat cttcgtctgc ttcggcgtgg cgatgactgg catcggcatg ctgacgttga
     3661 ccggcaacac gatctcgatg gacgtcttcg gccccgttgc cgacaac
//
LOCUS       DATIMH010000903         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3391810, whole genome shotgun sequence.
ACCESSION   DATIMH010000903 DATIMH010000000
VERSION     DATIMH010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3391810"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..587)
                     /locus_tag="VLI90_15670"
     CDS             complement(240..587)
                     /locus_tag="VLI90_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15700.1"
                     /translation="MRRHSPYLLCAALAAWCVAGGCSEPRGPLSVKSDDPDLKIPAIK
                     QDVRQRNTRDTAQMVKDLNDDDPAVRFYAIEGLRRLTGQTFDYHYYDDPDQRQPAIKR
                     WRQWLAQQSATKK"
     gene            complement(653..1807)
                     /locus_tag="VLI90_15675"
     CDS             complement(653..1807)
                     /locus_tag="VLI90_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15701.1"
                     /translation="MSNHDKQIPALLIVMCSAILLARPIASAADLATNSSKLRVAVFP
                     LAGSASPDQREKVGFALRAKLDRDDHYEPIDGPTMQEAAGDRPIAWDISIDALRKLVQ
                     DEQPQVLVWGQLDSSAGGLQLRLKTFDVDQPDPLPHEFQKTIAQPTDVRFAVEDILQT
                     LQGVKRFEHPVEDAVHDDAKARELFAHGVNLVLNGDFSKPSGWEALLGPDIYPPAMSA
                     TLPPVDKVVIYSMPDDKGGTNNVLAMNLSKDTAENNGLACLSAPTPIKPDMRYRLRFR
                     YRSDGPTLHVFVKGYTTAPGLDGKPEERECYRRQVPPSGATDGKWVTVDCDLNPQHVV
                     MKVEHLRVDLYAYLKPGVVMFDDVELKAVGEQTRHAADAAIKPPATRPAK"
     gene            1946..2173
                     /locus_tag="VLI90_15680"
     CDS             1946..2173
                     /locus_tag="VLI90_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15702.1"
                     /translation="MPAAQRHQHFRQRIRRQLTQTAAASARRFITSGRLISSNSSGTS
                     APGFKNLGLGTTELRYTLRGDASLDGTHQCG"
     gene            2312..>2692
                     /locus_tag="VLI90_15685"
     CDS             2312..>2692
                     /locus_tag="VLI90_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15703.1"
                     /translation="MLAHTLKILFVTDLLPGFMGMGAMQRATHLYESLAKIGDVDTFL
                     LVNESFDPAKWPEENRRRFRMVGYARMPKRGERGLWRNVRPVAPKLVDRVSNAIGSPA
                     AQYEPYAATADALRRCIAAGNYDVV"
BASE COUNT          493 a          884 c          808 g          507 t
ORIGIN      
        1 gacttgcgca ccgccgaaag gttcgcggga ttggaggtga acttgaagcc gagtttttcg
       61 gatgagcggt cctcggcgat ggaatccacc gcgccgtcgg gtccatcggg cgccaccatc
      121 gcgcgcaggg cgtcgagttg atttgcgatc cattcagaaa aacgcatgtt cacccgaggg
      181 ataacaaccc gcgcccgcgc gggcgcgggc gcctggtcct gccatgctgc acatcgccgc
      241 tactttttcg ttgcgctctg ctgcgccaac cattgtcgcc agcgtttgat cgccggctgg
      301 cgctggtctg ggtcatcata gtaatggtag tcgaacgtct gaccggtcaa ccgccgcaat
      361 ccttctatcg cataaaaccg caccgcgggg tcgtcgtcgt tcagatcctt gaccatctgg
      421 gccgtatcac gcgtgttgcg ctggcggacg tcctgcttga tggcgggaat cttcaggtcc
      481 ggatcatcgc tcttcacgct gagcggcccg cgcggctcgc tgcacccacc ggccacgcac
      541 cacgcggcca gcgcggcgca taaaaggtaa ggcgaatggc ggcgcatctc tggttgttga
      601 tcgtatgcgg acgaacgctt tctgcaatga atcgcgagca ccgtccgata actcacttcg
      661 ctggtctggt ggccggtggt ttgatcgccg cgtcggcggc atgacgcgtc tgctccccca
      721 ccgccttcag ctcgacatcg tcaaacatga ccacgcccgg cttgagatac gcatacagat
      781 cgacccgcag atgttcaact ttcatgacga cgtgctgcgg gttcagatcg caatcaacgg
      841 tcacccactt gccatcggtc gccccgctcg gcgggacctg ccggcggtaa cattcgcgct
      901 cctccggttt gccatcaagt cccggcgccg tcgtgtatcc cttcacgaac acgtgcagcg
      961 ttggaccatc gctgcgatag cgaaaccgca gacgatagcg catgtccggc ttgatcggag
     1021 tcggcgccga taaacacgcc agcccgttgt tctccgccgt atctttcgac aggttcatcg
     1081 ccaggacgtt gttcgtgccg cctttgtcgt ccggcatcga atagatgacg accttgtcca
     1141 cgggcggcaa cgtcgcgctc atcgccggcg gatagatatc tggcccgagt agagcctccc
     1201 aaccactcgg tttagaaaaa tcgccgttga ggacgaggtt gacgccgtgc gcgaagagct
     1261 cgcgcgcctt cgcgtcatcg tgcacggcgt cttccaccgg atgctcgaac cgcttcacgc
     1321 cttgcagcgt ctgcaggatg tcctcgaccg caaaacgcac gtccgtcggc tgtgcgatgg
     1381 tcttctggaa ctcatgcggc aacgggtcgg gctgatcgac atcaaatgtt ttgagccgaa
     1441 gctgcagacc gccggccgag gagtcgagct gtccccacac cagcacctgc ggctgttcat
     1501 cctggaccag cttgcgcagc gcatcaatgg aaatgtccca cgcgatcggt ctatcccccg
     1561 ccgcctcctg catcgtggga ccgtcgatcg gctcgtaatg atcgtcgcga tcgagcttgg
     1621 ctcgaagcgc gaagcccacc ttctcgcgtt gatcgggcga tgccgaaccg gcgagcggaa
     1681 agacggcaac cctgagcttg ctggagttgg tcgccaagtc ggcagcgctg gcgatcggcc
     1741 gcgccaacag tatcgcgctg cacatgacga tcaacaacgc ggggatttgc ttatcatggt
     1801 tgctcattct gaatgcctgc ccgagccgcg cacggagcgt actcgcggtg taagcggatc
     1861 cgcccgcgcg acgctggccg caccgcaaca atgggagacg gccgcgcgtt cgtcaatacc
     1921 gcggtgactg cgctctttca aagcgatgcc ggcagcccaa cggcaccaac attttcgcca
     1981 acgcatccgg cggcagctga cgcagacggc ggcggcgtcg gctcgcaggt tcatcaccag
     2041 cgggcgattg atctcgtcca acagcagcgg cacctcagcg cccggcttca agaacctcgg
     2101 cctcggcacc accgaactca ggtacacgct gcgcggcgac gcgagcttgg acggcaccca
     2161 ccaatgtggg taacctcggc cgcactggca acgaattaca gcatcacctc cggtggcgaa
     2221 accgctcagc ccgccgtcat aaacggtaga gattgcccat tccaacgcga cgcgctccct
     2281 cgatagattc cgttttttca tcgagggcgc cgtgctggcc cacacgctga agatcctgtt
     2341 cgtcaccgac ctgttgcccg gcttcatggg catgggcgcg atgcagcgcg cgacgcacct
     2401 gtacgaatcg ctggcgaaga tcggcgacgt cgacacgttc ctgctcgtca acgaatcatt
     2461 cgatccagcc aagtggccgg aggaaaaccg ccggcgattc agaatggtcg gttacgcgcg
     2521 catgcccaag cgcggcgagc gcggcctgtg gcgtaacgtt cgaccggtgg cgccgaagct
     2581 ggtcgatcgc gtgagcaatg cgatcggcag cccggcggcg cagtatgagc cgtacgcagc
     2641 gacagccgac gcgctgcggc ggtgcatcgc cgccggtaac tacgacgtcg tc
//
LOCUS       DATIMH010000904         1724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5705365, whole genome shotgun sequence.
ACCESSION   DATIMH010000904 DATIMH010000000
VERSION     DATIMH010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5705365"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VLI90_15690"
     CDS             complement(<1..272)
                     /locus_tag="VLI90_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15704.1"
                     /translation="MASLAQETRLAFSADDRFFVRGAIIMALTIFAAFTFQHAMGRSS
                     FASPPLVHAHAIIFMGWVVIYLTQNLLIGTGRVDIHRKLGWIALGW"
     gene            392..1303
                     /locus_tag="VLI90_15695"
     CDS             392..1303
                     /locus_tag="VLI90_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSV15705.1"
                     /translation="MIDAHSTLYAHWMLPRVPDRSRDRRIEDPSNLWIIHPAAHWLLP
                     WFLKRGISANAVSLGGLCLGALAALSFANWHVRPFAWIGLLLSMAWLVADGLDGMIAR
                     ATGTASAVGRFLDGICDHGVFILIYVALAVSIGTVDAWILGACAGVFHALQSNLYEGE
                     RARFHRRCMGIAGATAAPGGNPMVRMYDQVSGVVDRFALRLDAVLGRQANPVQFGAAY
                     GAAAAKPLRLMSLLSANVRVYAIFLACMAREPRLFWWIEILPMTAILIIGLTWHRAVE
                     SGLIRNMGATTASIPSSTSIHSKDVTN"
     gene            1408..>1724
                     /locus_tag="VLI90_15700"
     CDS             1408..>1724
                     /locus_tag="VLI90_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15706.1"
                     /translation="MHWEVGNYRPSDIHVVAAWDVDRRKVGKDVAEAIFAKPNCTEVF
                     CADVQPSGAVVRMGRKLDGVADHMADYPADRTFDVAVEAEPDRDDVVRVLKESQADVL
                     MNYL"
BASE COUNT          321 a          514 c          553 g          336 t
ORIGIN      
        1 atccacccca aagcgatcca gccgagcttg cggtggatgt cgacgcggcc ggtgccgatc
       61 agcagattct gggtgagata gatgacaacc cagcccatga agatgatcgc atgggcgtgg
      121 accaggggcg gcgaagcgaa gctcgagcgg cccattgcat gctgaaaggt gaaagcggcg
      181 aaaatggtga gtgccatgat gatggcgccg cggacgaaga aacggtcgtc ggcgctgaac
      241 gcaagtcgag tttcttgagc tagactggcc atgcttaccc ccgagtttgg cggccaaaat
      301 tctacgccgt tgagagcttc gcgaaaagcc ccgcattcaa tgggaggtcg tcttcgctgc
      361 agccctgtga agacaacacg ccgctaacca cttgattgac gcgcactcga cgctgtacgc
      421 gcactggatg ttacctcgtg ttccggaccg gtcgcgggat cgccggatcg aggatccgag
      481 caacctgtgg atcatacacc ccgcggcgca ttggctgctg ccttggtttc tcaagcgggg
      541 catatccgcg aacgcggtgt cgctcggcgg gctatgcctc ggcgctcttg cggcgctttc
      601 attcgccaac tggcacgtgc ggccgttcgc gtggatcgga ttgctcctgt cgatggcctg
      661 gctcgtcgcc gatgggctgg acggcatgat cgcccgggca accggcaccg cgagcgcggt
      721 aggacgattt ctcgacggga tctgcgatca cggtgttttc attctgatct acgtcgcctt
      781 ggcggtatcg atcggaaccg tggacgcgtg gatattgggc gcttgcgccg gggtgtttca
      841 tgcgctgcaa tccaacctgt atgaaggcga acgcgcgcgg tttcaccgcc gctgcatggg
      901 catcgcggga gcgactgcag cgcccggcgg caacccgatg gtgcgcatgt acgaccaggt
      961 ctccggggtg gtcgacaggt tcgcgctgcg cctcgacgca gtgctgggtc ggcaggcgaa
     1021 tccggtgcag tttggagccg catatggcgc cgccgccgcc aagccactac ggctgatgag
     1081 cctgctcagc gccaatgtcc gggtctatgc catcttcctt gcctgcatgg cacgagagcc
     1141 tcgccttttt tggtggatcg aaatcctgcc tatgacggcg atcctcatca tcggtcttac
     1201 ctggcaccgc gctgtggaat ccgggctcat ccgcaacatg ggcgcgacga cagcgtcaat
     1261 tccaagctca acatcgatcc attcgaagga cgtgacaaat taatgccgaa catcaacatt
     1321 gcaatcgttg gcgttggaaa ctgcgccagc tctctcgttc agggtctctc ttactatcgt
     1381 cgcggcgcga acgagacggt tgggctgatg cactgggagg tcggcaacta tcgtcccagc
     1441 gacatccacg tcgtcgccgc ctgggacgtt gaccgacgca aagtcggcaa ggacgtcgcc
     1501 gaggcgatct tcgctaagcc aaactgcacc gaagtgttct gcgccgacgt ccagccgagc
     1561 ggcgcggtgg tccgcatggg ccgcaagctc gatggggtcg cggatcacat ggccgattat
     1621 ccggcggacc ggacgttcga cgtggcggtc gaagcggagc ccgaccgcga cgacgtcgtg
     1681 cgcgtactga aggaatcgca agccgacgtg ctgatgaact atct
//
LOCUS       DATIMH010000905         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3105180, whole genome shotgun sequence.
ACCESSION   DATIMH010000905 DATIMH010000000
VERSION     DATIMH010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3105180"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VLI90_15705"
     CDS             <1..1050
                     /locus_tag="VLI90_15705"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HSV15707.1"
                     /translation="ENAALKAKLESGEALFGTIDTYLLYRLTGGKTFATDSTNASRTL
                     LYNINTLSWDSELCKLFGVPIRALPAVRQSSARFGECTDSTLPAGTPIAGVMGDSQAA
                     LFAHRCFEPGMAKVTLGTGSSLLLNTGAHLPATGIRTLAWSRDGAATYSVEGIISYSA
                     ATIGWLKNQLGLIRSADETEAAARAVADNGGVYLVPAFNGLAAPHWSPKARAAIVGLS
                     AASTRNHVIRAALESIAFQVKDALDSMIGDSRVSLTHLTADGGASRNGFLMQLMADLL
                     GTPITVCDLPDFSPLGAAYAGMLAMGLCNSLDDLHRLPMRERTYTPAMSRAAAAELHA
                     GWTNAVRQVLAGVSG"
     gene            complement(1318..1728)
                     /locus_tag="VLI90_15710"
     CDS             complement(1318..1728)
                     /locus_tag="VLI90_15710"
                     /inference="COORDINATES: protein motif:HMM:NF021902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSV15708.1"
                     /translation="MASILERVTFPMIADPQLKVAKAYGMLPAEAGETCEGRTAAHNA
                     TVRTVFVVGPDKRIQLTLAYPMSTGRNFDEVLRVIDSMQLTAKHKVATPANWKPGGDV
                     IILPSVSDEDAKKKFPGGWKAPRPYLRIVEQPTA"
     gene            complement(1704..2078)
                     /locus_tag="VLI90_15715"
     CDS             complement(1704..2078)
                     /locus_tag="VLI90_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15709.1"
                     /translation="MRIQLNFVYNWEDLRSAVHFFSAPAVPKAVKWIRFAFLAIVLGS
                     RALVVVGFMRHWPAERMAVPICIVLAVFVLRKVNAMRAAAIRSGWIGASPRITPLLDE
                     DGVAITRRKRTVIYSWPAFLSG"
     gene            complement(2519..>3260)
                     /locus_tag="VLI90_15720"
     CDS             complement(2519..>3260)
                     /locus_tag="VLI90_15720"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSV15710.1"
                     /translation="LDFYRAAGAAGRSPRPCVVVIHGGSWVSGNRLDDGTKRWLNDWL
                     AGLGYAVASIDYRLSPQFKWPAQREDLVAAIGFLRAHAADLGIDGDRLVLVGRSAGAQ
                     MAIATAYAEVIPGIRGIIDIYGPTDFDLTWDAATRPRSLDHRYNLEIFLGGNPETARD
                     AYRSASGAMLVTSRAPPTLILHGALDINVFPEQAELLDRKLAAAGVPHALVSLPWAGH
                     AFDFVNFNTPSAQIGRYAIVRFLAGVMR"
BASE COUNT          586 a         1097 c         1024 g          553 t
ORIGIN      
        1 gaaaacgccg cgctgaaggc gaagctcgaa tccggggaag cgctgttcgg cacgatcgac
       61 acatacctgc tctaccggct gaccggcggc aagacgttcg ccaccgattc caccaacgcg
      121 tcgcgcacgc tgctgtacaa catcaacacg ctcagttggg actcggagct ctgcaaattg
      181 tttggcgtcc cgatacgcgc gctgccggcg gtgcggcaga gcagcgcccg ctttggagaa
      241 tgtactgact cgacattgcc cgcggggacg ccgatcgccg gcgtcatggg cgattcgcag
      301 gcggcgctat ttgcgcaccg ctgcttcgag ccgggcatgg cgaaggtgac gctcggcacc
      361 ggctcttcgc tgctgctcaa caccggcgcg catctgccgg ccaccggcat tcgcacgctc
      421 gcgtggtcgc gcgacggcgc ggccacgtac agcgtcgaag gcatcatctc ctactcggcc
      481 gccaccatcg gctggctgaa gaaccagctc gggctgatcc gctccgccga cgaaaccgaa
      541 gccgccgccc gggcggtcgc cgataacggc ggcgtttatc tggtgcccgc gttcaacggg
      601 ctggcggcgc cgcattggtc gccgaaggcg cgggccgcca tcgtcggtct atccgcggcc
      661 tccacgcgca accacgtcat tcgcgctgcg ttggaatcca tcgcgtttca ggtgaaggat
      721 gcgctggatt cgatgatcgg cgacagccgg gtgtcgctga cgcacttaac cgccgacggc
      781 ggggcgtcgc ggaacggatt tctgatgcag ctgatggccg acctgctcgg cacaccgatc
      841 accgtgtgtg atctgccgga tttctcgccg ctcggcgcgg cgtatgccgg catgctcgcg
      901 atgggccttt gcaattcgct agacgatctt catcgcctgc cgatgcggga gcggacgtac
      961 acgccggcga tgtcgcgcgc cgccgccgcc gagctacacg ccggatggac caacgccgtc
     1021 cgccaagtgt tggcgggggt gtcggggtga ggtgggagcg gaattaacca cgaagggcac
     1081 gaatggcgcg cgtggatatc ccgagcgcac gcgtacttgc cggagtcgaa ggatctcgct
     1141 ggaacgtcag cgaactgctg ctgaggaggg acgcgcagta gcggcttgcc cttggcagcg
     1201 ccgcgaaggg caaaccactg ctgcgtgtgc gagacacgct tcacatattt ctcggcaaga
     1261 tccttcgact ccggcgagta cgcctgcgct ggaggatgac aagcgcgccc ccgctcacta
     1321 cgccgtcggc tgctcgacga tccgcagata cggccgcggg gccttccagc cgccggggaa
     1381 ctttttcttc gcatcctcat ccgacactga cggcaagatg atgacgtcgc cgcccggctt
     1441 ccagttcgcc ggcgtcgcca ccttgtgctt ggccgtcagc tgcatcgaat cgatcacccg
     1501 gagcacttcg tcgaaattcc gccccgtgct catcgggtac gccagcgtca attggatccg
     1561 cttgtccggc cccaccacga agaccgttcg taccgtcgca ttatgcgccg cggtccgccc
     1621 ctcacatgtc tcgcccgcct cggcgggcaa catgccgtac gccttggcca ccttcagctg
     1681 cgggtcggcg atcatcggaa acgtcacccg ctcaagaatg ctggccaaga atagatgacg
     1741 gtcctcttgc gccgggtaat cgccactcca tcctcatcga gaagtggtgt gatgcgggga
     1801 ctggcgccga tccagcccga gcgaatcgcc gcagcgcgca ttgcattgac tttgcggagc
     1861 acgaacaccg cgagcacgat gcaaatcgga accgccattc gttcggctgg ccagtgccgc
     1921 ataaaaccga cgaccaccag agcccggctg cccagaacaa tcgccaggaa ggcgaatcga
     1981 atccacttta ccgccttggg cacagccggc gcggagaaga agtgaacagc gctgcgcagg
     2041 tcttcccaat tgtaaacgaa gttgagttgg atgcgcacct gtttactccc tgactcgtac
     2101 cataagttgt ctgtatgctc gcaccccttc gacaatgatg ctcacaccgc ccgtggtact
     2161 tcagattagc agaatcgatg aaacggaagc aattggtgtg gacgcccatc ctgccgcccg
     2221 ttaagtcgcc ccgcgcagga taggatgtgc catgtgcgcg cgcgagctcg tgacatgaaa
     2281 caccgagcac gtgcgaccga cgcccattcg tgcagggagc ccaccatgcc caagtaccgc
     2341 cgctcgaagc gcacgtgcga tcaccagaca ttcaagcgtc gttgaactta accgaggcca
     2401 cggctctcaa agcgcgacga cgccgaacct gcgcgccaat atcacgcttg gcggggggcg
     2461 ggattcatgg tcggcgggta atgccgcggc cgctctgctc attgccggtc gcttccggtc
     2521 atcgcatgac gcccgccagg aagcgtacga tcgcatacct gccgatctgc gcactgggcg
     2581 tgttgaagtt gacgaagtcg aacgcatggc cggcccacgg cagcgacacc agcgcgtgcg
     2641 gcacaccggc cgcggcgagc ttccggtcga gcagctccgc ctgctccggg aacacgttga
     2701 tgtccagcgc accgtggagg atcagtgtcg gcggagcgcg cgaggtgacg agcatggcgc
     2761 cgcttgcgct ccgataggcg tcgcgcgccg tctccgggtt gccgcctaag aatatttcga
     2821 ggttgtagcg gtggtcgagg ctgcgcgggc gggtggccgc atcccacgtc aggtcgaagt
     2881 ccgtcggccc gtagatgtcg atgatgccgc ggatgccggg aatcacctcc gcataagcgg
     2941 tcgcaattgc catctgcgcg cccgccgacc gtccgaccag cacgagccga tcgccgtcga
     3001 tgcccaaatc agctgcgtgc gcgcgcagga acccgatggc ggcgacaagg tcctcgcgct
     3061 gcgccggcca cttgaactgc ggcgacagcc ggtagtcgat cgacgccacc gcgtacccca
     3121 gccccgcgag ccagtcgttg agccagcgct tggtgccgtc gtccagccgg ttcccgctca
     3181 cccagctccc gccatgaatc acgaccacac acggccgggg tgaacggcct gcggcccccg
     3241 cggcgcggta gaaatccagc
//
LOCUS       DATIMH010000906         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3683536, whole genome shotgun sequence.
ACCESSION   DATIMH010000906 DATIMH010000000
VERSION     DATIMH010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3683536"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VLI90_15725"
     CDS             <1..1373
                     /locus_tag="VLI90_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15711.1"
                     /translation="LPAGGMRIGVLTNNLSVFLQALESVTNTTVLSNPKVLALDKQKG
                     EVNVGAQLGYKTTTTSSTTTTETVAFLPTGTRLIFRPFIGDDGFIRLEIHPEDSSGTI
                     DAAGVPQVTTTEVTSNVMVKDGHTIVIGGLFRDVSSASRGQVPGIGNIPFLGALFRQQ
                     QDSTQREEVIILLTPHIIKDDAAYSALSEEQQKRIEDYRVGVRKGMMIWGRERLAETA
                     YQNALASLREPNPNRGMAMWHLNNATNLNPQFVEAMELKEKISGREVTASDNSSIRQF
                     VSKAIISDQGTLQTPPPVMDIMPRRSTTMPTSTAGVATSQPAAVTPVAAVPIMEQFPA
                     TQPTTEPSLVELTDATTQPTSQPAVAEDDGGTAPTSTVTAISDDASAQPATQPSGGDE
                     LVRAAKALAEAAGHMANAADDLAHYAEVQPGHAKPSAMPTTRRTSKPSNGGTVTVVPL
                     DDGNGN"
     gene            1481..2671
                     /locus_tag="VLI90_15730"
     CDS             1481..2671
                     /locus_tag="VLI90_15730"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV15712.1"
                     /translation="MRLNKRAACLVVGLAIVAGCGVKPHPSPHEQGSKQWNDARAGIL
                     GTLAADQYKNGNFEKCEESLGEALRLAPDNVNLHILAAKLNIEQGKLEPAESQLAVAG
                     KLDPKNPEVDYLHGVVLQRWQQPQKAYEAYDAAVQKNPQELAYLMAKSEMLVALDRTD
                     EALALLQAKVVYFEHSAAIRDAVGQLLVEQGKYTEGAAMLREASILASDDQTIREHLG
                     FALYFAKQYAEAAEVFTRLVKTEERAKRADIFAALGECQCQMGQLRQARQSLASATQI
                     DQGSCGYWLALGRITSQLGDLPRAEIAARKALSLEPANSEAHCLIGYVRLREGKLPQS
                     LSAFRMASQLDASDAVSVTMQGYVLQKMGKHDEAVQLYAKALRMKPGDELANRLMASV
                     NVNE"
     gene            2789..>4273
                     /locus_tag="VLI90_15735"
     CDS             2789..>4273
                     /locus_tag="VLI90_15735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV15713.1"
                     /translation="MRRGTVNLVLVVGLLIGGLIALHSGSAGGIVHALRDLPVGQLTA
                     VTLFILAAGTGLFGQLSVRASAGQLTKQVHDAAASGGASRVDRLHPDLASLAGAVNEV
                     IDAAEQTVARARLAVKELEIQLKVATAERQHAEAIIYSISDAVLVTDPFDELVLANES
                     AARTFDFNLDAASRSPVHEVLRDPKMIELIRDMRQSNSRTGRRIVEHQVRTPLGERVF
                     KVTLSCVADQVSPEDRPVLERSGGSAGGDHNAGVVAVLHDMTREKEVAEMKNDFVSSV
                     SHELRTPLASIKAYVEMLIDGEADDERTRREFYDVIQNEANRLSRLIDNILNISRIES
                     GLVKINKHPQSLAVILKDAVEVIAPQAKLKGIEIVERLTPIFYQTFCDKDMLYQAVLN
                     LLSNAVKYTPEKGIITVETTVDETNKKVVTRISDTGVGIPSAEIPFLFDKFYRVESNS
                     HMAKGTGLGLSLVKHIVETVHKGRMFVESNVGKGSCFGFELNLCD"
BASE COUNT          861 a         1122 c         1576 g          714 t
ORIGIN      
        1 agctgcctgc cggcggcatg cgcatcggcg tgctgaccaa caacctgagc gtgtttctgc
       61 aggcattgga atcggtgacg aacacgacgg tgttgtcgaa cccgaaggtg ctggcgctgg
      121 acaagcaaaa gggcgaagtg aacgtcggcg cgcagctggg ttacaagacg accacgacca
      181 gctcgaccac gacaaccgag acggtggcgt tcctgccgac cggcacacgg ctgatcttcc
      241 ggccgttcat cggtgacgac gggttcatcc gcctggaaat ccacccggag gactcgagcg
      301 gcacgatcga cgcggccggc gttccgcagg tgacgacgac cgaagtgacc agcaacgtga
      361 tggtgaagga cggtcacacg atcgtgatcg gcgggttgtt ccgcgacgtc agcagcgcgt
      421 cgcgcgggca ggtgccgggc atcggcaaca tcccgttcct cggggcgctg ttccgccagc
      481 agcaggacag cacgcagcgc gaggaagtga tcatcctgct gacgccgcac atcatcaagg
      541 acgacgccgc gtattcggcg ttgtccgaag agcagcagaa gcggatcgaa gattatcggg
      601 tgggcgttcg caaggggatg atgatctggg gccgcgagcg tttggcggag acggcgtatc
      661 agaatgcgct ggcatcgctg cgcgagccga atccgaaccg cgggatggcg atgtggcatt
      721 tgaacaatgc cacgaacctg aacccgcagt tcgtggaggc gatggagctg aaggaaaaga
      781 tcagcgggcg cgaagtgacg gcatcagata acagttcgat ccggcaattc gtgagcaagg
      841 cgatcatcag cgaccagggg acgttgcaga ccccgccgcc ggtgatggac atcatgccga
      901 ggcgttcgac gacgatgccg acgtcaacgg cgggggtggc gacgagccag ccggccgcgg
      961 tgacgccggt ggcggcggtg ccgatcatgg agcagttccc ggcgacgcaa ccgacgacgg
     1021 aaccgtcgtt ggtggagctg acggacgcga cgacgcagcc gaccagccag cccgcggtgg
     1081 cggaggacga cggcgggact gcgccgactt cgacggtgac ggcgatctcg gatgacgcga
     1141 gcgcgcaacc ggcgacgcaa ccgagcggtg gcgatgagtt ggttcgcgcg gccaaggcgc
     1201 tggctgaggc ggcggggcac atggcgaatg cggcggatga cctggcgcat tatgccgagg
     1261 tccaaccggg ccatgccaag ccaagcgcga tgccgacgac gcgtcggacg agcaagccga
     1321 gcaacggcgg cacggtgacg gtggtgccgt tggatgacgg gaatggtaac tgagccgcgc
     1381 ggcgtggcgt ggccgcttta gcgaccatgt ctgtgccgcg cgtctcaaca ggatgcatgg
     1441 tcgctcgagc gatcacgccg cgttcgggaa tggagtgctc atgcggttga acaagcgcgc
     1501 agcgtgtttg gttgttggct tggcgatagt agcgggatgc ggtgtgaagc cgcatccgtc
     1561 gccgcacgag cagggcagca agcagtggaa cgatgcgcgc gccggcattc tgggcacgct
     1621 tgccgccgac cagtacaaga acggcaactt cgagaagtgc gaggagtcgc tgggcgaagc
     1681 gctgcggctg gcgccggaca acgtgaacct gcacatcctg gcggcgaagc tgaacatcga
     1741 gcagggcaag ctggagccgg ctgagagtca gctggcggtg gcgggcaagc tcgatccgaa
     1801 aaatccggag gtggattatc tgcacggcgt ggtgctgcag cgctggcagc agccgcagaa
     1861 ggcatacgag gcgtacgacg cggcggtgca gaagaacccg caggagctgg cgtatctgat
     1921 ggccaagtcg gagatgctgg tggcgctgga tcggaccgac gaagcgctgg cgctgctgca
     1981 ggcgaaggtg gtttacttcg agcacagtgc ggcgattcgt gatgcggtgg ggcaattgct
     2041 ggtcgagcag ggcaagtaca ccgagggggc cgcgatgctg cgcgaggcga gcatcctggc
     2101 gagcgacgat caaacgattc gcgagcacct ggggttcgcg ctgtatttcg cgaagcaata
     2161 tgccgaggcg gccgaggtgt tcacgcggct ggtgaagacc gaagagcgcg ccaagcgtgc
     2221 ggatatcttc gcggcgttgg gtgaatgcca atgccagatg ggccagctgc ggcaggcgcg
     2281 ccagagcctg gcgtcggcga cgcagatcga ccagggcagc tgcggttatt ggctggcgct
     2341 ggggcggatc acgtcccaac tgggcgattt gccgcgggcg gaaatcgcgg cgcgcaaggc
     2401 gctgtcgttg gaaccggcta atagcgaggc gcattgcctg atcggttacg tgcggctgcg
     2461 cgaggggaag ctgccgcagt cgttatcggc cttccggatg gcgagtcaat tggacgcgag
     2521 tgacgcggtc agcgtgacga tgcaggggta tgtgcttcag aagatgggga agcacgacga
     2581 ggctgttcag ctctacgcca aggcgctgcg gatgaaaccg ggcgatgagt tggcgaatcg
     2641 gttgatggcg tcggtgaatg tgaatgagtg atgagtgagg attggccgaa cgatgagcga
     2701 tgaacgatga acgatgagcg gagaacggca atctccgaat tcatccttca tcgttcatcg
     2761 ctcatcgttt gttgaatgga gtccgctgtt gcgtcgtggc acagtcaatc tcgtgctggt
     2821 ggtcggcctg ctgattggcg ggctgattgc gctgcatagc gggtcggccg gcggcatcgt
     2881 tcatgcgctg cgcgatctgc cggtggggca attgacggcg gtgacgctgt tcatcctggc
     2941 ggcggggacg ggattgttcg ggcagttgtc ggtgcgcgca tcggcgggtc aattgacgaa
     3001 gcaagtgcac gatgcggcgg cgtctggggg cgcgagtcgc gtcgatcggt tgcatccgga
     3061 tctggcttca ttggcgggcg cggtcaacga agtgatcgac gcggcggagc agaccgtggc
     3121 gcgggcgcgg ctggcggtga aggaactgga gatccagctg aaggtggcga cggccgagcg
     3181 gcagcacgcg gaggcgatca tctacagcat ctccgatgcg gtgctggtga cggatccatt
     3241 tgacgaactc gtgctcgcta atgaatcggc ggcgcggacg ttcgatttca atctggatgc
     3301 ggcgagccga tcgccggtgc acgaggtgct gcgcgatccg aagatgatcg agttgatccg
     3361 cgacatgcgg cagagcaaca gccgcacggg acggcggatc gtggagcacc aggtgcgcac
     3421 gccgctgggc gagcgcgtgt tcaaggtgac gctgtcgtgc gtggcggatc aagtctcgcc
     3481 ggaagatcga ccggtgctgg agcgcagcgg cggcagcgcg ggcggcgatc acaacgccgg
     3541 cgtggtggcg gtgcttcacg acatgacgcg cgagaaggaa gtcgcggaga tgaagaacga
     3601 cttcgtctcc agcgtcagcc acgaactgcg cacgccgctg gcgagcatca aggcgtacgt
     3661 cgagatgctg atcgacggcg aggccgacga cgagcgcacg cggcgcgagt tttatgacgt
     3721 gattcagaat gaggcgaacc ggctgtcgcg gttgatcgac aacattttga acatctcgcg
     3781 catcgaatcg gggctggtga agatcaacaa acacccgcaa agcctggcgg tgattttgaa
     3841 ggacgcggtg gaagtgatcg cgccgcaggc gaagttgaag ggcatcgaga tcgtggaacg
     3901 gctgacgccg atcttttatc agacgttttg tgacaaggac atgctgtacc aggcggtgct
     3961 gaacctgctg agcaacgcgg tgaagtacac gccggaaaaa ggaattatca cggtagagac
     4021 aaccgtcgat gagacaaata agaaagtcgt gacgcgcatc agcgacacgg gggtgggcat
     4081 tccgtcggcg gaaattccgt tcctgttcga caagttttat cgagtcgaat cgaactcgca
     4141 catggccaag gggacgggac tgggcttgtc gctggtgaag cacattgtgg aaaccgttca
     4201 caagggacgg atgtttgtcg agagcaacgt gggcaaggga agttgttttg ggtttgaact
     4261 gaatctgtgc gat
//
LOCUS       DATIMH010000907         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1663647, whole genome shotgun sequence.
ACCESSION   DATIMH010000907 DATIMH010000000
VERSION     DATIMH010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663647"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VLI90_15740"
     CDS             <1..817
                     /locus_tag="VLI90_15740"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSV15714.1"
                     /translation="VDDFKLVLGMPVEQDVEIVPVALEVAIPSISSDEANALAERYRL
                     SLQTARDRIDDARRNISNAENGLLPDLSLTGSSSVGNRTGTPAKELDSRTLAYSAGLR
                     LDLPVDRLAERNTYRAALLDFAKAQRDYVDETENVRADVRTSLRQIQTAQLNIQIQRR
                     AVELAQRRLEYSTLLLQLGTLSSNRDLVDAQTSLLSAQDAYEQAKSDLQVNVLQYLRN
                     TGTLRVDPRAGTLGRALNMTDNELRDGDSPPTLNSTGGAISTAEPAAKSPSAG"
     gene            895..2505
                     /locus_tag="VLI90_15745"
     CDS             895..2505
                     /locus_tag="VLI90_15745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSV15715.1"
                     /translation="MSNVAIAASVPGTRRWTGWLIAAVVAMIAVGGYAAYTRWAGDGA
                     GQIVSGDFYNVQPIDLVQHIIKDGELQAVNNIDIICQVEGQSTINTLVKEGTFAKKGD
                     VLVTLDSSAIRQKMDDTTLALQTSEADVTNAREAKEIQESQNDANMDAATVALNLAKL
                     DYAQYVDGTYPQTLANANTDLEMAKITLRNSEEDYANAKSLYAKGFVTGADIQKSELA
                     LTNARNGVSKAQTALDVLTKYTHAEDLASKQNAVAQAEKSLMRTKQQNQANLAQKTSD
                     FNAKQEAFKLQQRKMEHLQEQFEDCTIKAPADGMVVYASSGDRNAQNPIQEGATVRER
                     QPLLRLPDTSSMMASIRVPEGSVGSLREGQRAMVNIVNVPRPLPAKVTRISVLVDSGQ
                     RWWNPDLKEYPVDLTLDLTPRGLKPGMGCRSEVFIDRIPNALAVPLPAIYASGQDSYV
                     FVRSEETVRPVKVKLGATNETHAQILDGLTAGDQVLVLQAGQGRELLEKNGIKVASPT
                     TKPSRGEKHRKPDDAPTAPTAGDAKPAV"
     gene            complement(2766..>3194)
                     /locus_tag="VLI90_15750"
     CDS             complement(2766..>3194)
                     /locus_tag="VLI90_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15716.1"
                     /translation="DVSAVTATIDTGTATYPNCDETGEGLLRFENGAIGTLAGAWDDL
                     ANPLTYLVSGTEAHAAVINGQVYFQTNRKGESSSPQPLAERELPPAKPAGFESWLNAI
                     SGQSDQALVSAREAAYRSAVMEALYQGAVQQTWVTPERRF"
BASE COUNT          665 a          982 c         1035 g          512 t
ORIGIN      
        1 cgtcgatgac ttcaagctgg tgctgggcat gccggtcgag caggacgtgg aaatcgtgcc
       61 ggtggcgctg gaggtggcga tcccgagcat ctcctccgat gaagccaacg cgctggcgga
      121 gcgctatcgg ctgtcgctgc agaccgcgcg cgatcgaatc gacgatgccc ggcgcaatat
      181 ttccaacgcc gagaacggcc tgctgccgga cctgtcgctg accggatcgt cgtcggtggg
      241 caatcgcacg ggtacgcccg cgaaggaact cgacagtcga acgctcgcgt atagcgcggg
      301 cctgcggctg gacctgccgg tcgatcgatt ggcggagcgc aacacctatc gcgccgcatt
      361 gctggatttt gcaaaagcgc agcgcgatta cgtggatgaa accgaaaacg tgcgggcgga
      421 tgtccgcacg agcctgcggc agattcagac cgctcaactc aacatccaga ttcaacgccg
      481 agccgtggaa cttgcacagc ggcggttgga gtattcgacg ctgctcctgc agttgggcac
      541 gttgagctcg aaccgcgatc tggtggacgc gcagacatca ctgctgagtg cccaggacgc
      601 ttacgaacag gcgaaatcgg atctgcaagt gaatgttttg caatatctta ggaacaccgg
      661 aacgctgcgc gtcgatccac gcgcgggcac gctgggccgg gcgctgaaca tgaccgataa
      721 cgaattgcgc gatggcgact cgccgccgac gctcaatagt accggtggtg caatttccac
      781 ggcggagccg gcggcgaaat cgccgtcggc gggatgaaca acccgggaag tcgcgcgtac
      841 tgtatagtcg gtaagagtcg gtggatcggg cagcagcagc aaaatcgggc aagcatgagt
      901 aatgtcgcaa tcgcagcttc agtgccgggc acccggcggt ggacgggatg gctgatcgcc
      961 gccgtcgtcg ccatgatcgc ggtgggtgga tacgccgcct acacgcgctg ggccggggac
     1021 ggcgcgggac aaatcgtttc tggcgatttc tacaacgtcc agccgatcga tctcgttcag
     1081 cacatcatca aggacggcga gctccaagcc gtcaacaaca tcgacatcat ctgccaggtc
     1141 gaaggtcaaa gcacgatcaa cacgcttgtg aaagaaggaa cattcgcgaa gaagggggac
     1201 gtgctggtca cgctcgactc gtccgccatc cggcagaaga tggatgacac gaccctggcg
     1261 ctgcagacca gcgaagccga cgtgaccaat gcgcgcgagg cgaaggagat ccaggaaagc
     1321 cagaacgacg ccaacatgga cgcggcgacg gtcgcgctga acctggcgaa gctggactac
     1381 gcgcaatacg tcgatggcac gtatccgcag acgctggcga acgccaacac cgatctggag
     1441 atggcgaaga tcacgctgcg gaattccgag gaagattacg ccaacgcgaa aagtttgtat
     1501 gccaagggat tcgtcaccgg cgcggatatt caaaaatccg agttggcgtt gaccaacgcg
     1561 cgcaacggcg tgtccaaggc gcagaccgcg ctggacgtgc tgacgaagta cacgcacgct
     1621 gaggacctgg cgagcaagca gaacgcggtg gcgcaggcgg aaaagtcgct gatgcgcacg
     1681 aagcagcaga accaggcgaa cctggcgcag aagacatcgg acttcaacgc caagcaggaa
     1741 gcgttcaagc tgcagcagcg caagatggag cacctgcagg agcagttcga agattgcacg
     1801 atcaaagcgc cggccgacgg gatggtggtc tacgcgtcca gtggcgatcg caacgcgcag
     1861 aacccgatcc aggaaggcgc gacggtccgc gagcgccagc cgctgcttcg cctgccggac
     1921 accagctcga tgatggccag catccgcgtg cccgaaggtt cggtcggttc gctgcgcgaa
     1981 ggccagcgcg cgatggtgaa catcgtcaac gtcccgcggc cgttgccggc gaaggtgacg
     2041 cgcatcagcg tgctggtgga cagcggtcag cgctggtgga atccggacct gaaggaatat
     2101 ccggtcgatc tcacgctcga tctgacgccc aggggcctga agcccggcat gggttgccgg
     2161 tcggaagtct tcatcgatcg cattcccaat gcgctggccg tcccgctccc ggcgatctac
     2221 gccagcgggc aggacagcta tgtcttcgtc cgctcggaag aaaccgtccg cccggtgaag
     2281 gtgaagctgg gcgcgacgaa cgaaacgcac gcgcagatcc ttgacgggtt aaccgccggc
     2341 gatcaagtgc tcgtgctgca ggcgggtcag ggccgcgagt tgctggagaa gaacggcatc
     2401 aaagtcgcat cgccgacgac caagccctcg cgtggcgaga agcatcgcaa gcccgacgac
     2461 gcccccaccg cccccacggc cggcgacgcc aagcccgcgg tttagagcag cacctcgtcc
     2521 tcctccagcg catccgctga gaaacgtgtt cggcggggag ggcggcacac tgtacctcgt
     2581 cgatcgcgac aagctcggtc acttcaactc cggcagcgac gcgcaggcat cgtgcagtcg
     2641 atccccagtt tcggccgcgt gtttcggcgc gccgatgtat ttcaacggca gcgcctacct
     2701 gcaggcggcc gacaggccgt tgaagcagag taccggaaga ggcgaccaga gcggctgcgt
     2761 gaagattaaa acctgcgttc gggcgtcacc cacgtctgct gcacagcgcc ttggtaaagc
     2821 gcttccatca ccgcactgcg atacgccgct tcgcgcgcac tcaccagcgc ttggtccgac
     2881 tgcccgctga tcgcgttcaa ccaactttcg aaccccgcag gcttcgccgg tggcagctcg
     2941 cgctccgcta gcggttgcgg cgaacttgat tcacctttgc gattcgtctg gaaatacacc
     3001 tgcccgttga tgacggccgc gtgggcttcg gtgccgctca cgaggtacgt cagcgggttg
     3061 gccaagtcgt cccacgcgcc ggccagcgtg ccgatcgcgc cgttctcaaa acgcagcagg
     3121 ccttcgccgg tttcatcgca attcggatag gtcgcggtgc cggtgtcgat cgtcgcggtg
     3181 acggcgctga cgtc
//
LOCUS       DATIMH010000908         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3967149, whole genome shotgun sequence.
ACCESSION   DATIMH010000908 DATIMH010000000
VERSION     DATIMH010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3967149"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1604)
                     /locus_tag="VLI90_15755"
     CDS             complement(189..1604)
                     /locus_tag="VLI90_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HSV15717.1"
                     /translation="MNATQRRSHRAFPLIILTAMLLASRGFASGAEAPDVFDVRDFGG
                     VGDGKTLNTAAFSRAVAAAEAAGGGTVRVGPGRYLTGTIMLKSNVTLDLDAAATLLAS
                     ESPDDFPLVDEVWTPGRQVRGPLIYAVDAQRVAVTGRGVIDGQGRKWWEPILAAKAKR
                     KAGAASSPATEPVASATSPGDWQYGRPRLIRFVRCSDVVVEHVTLQNAPEWNIHPLLC
                     ERIRIDGVTIFAPVPSPNTDGINVESCRNVQISNCRVDNGDDSITLKSGLDEAGRRMG
                     KPCEDITISNCVIYHGHGGVTIGSEMSGGVRNVTVSNCVFHGTDNGIRIKSQRGRGGV
                     VEGVAVSNIVMQDVPHPFTITTFYAGKDKPGDVYPVDAGTPTFRDFLFSSITARGAKE
                     AGSITGLREKPIADITFSNVHVRAEKGFTCTNADGIRFLDTRIEASQGEALIVKDCAN
                     LRTERLERITTQPASKMNEGG"
     gene            1860..>2175
                     /locus_tag="VLI90_15760"
     CDS             1860..>2175
                     /locus_tag="VLI90_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSV15718.1"
                     /translation="MSLRAAEPAAPADRPAGKIRIVLAGDSTTAVGSGWGPGFIKCLD
                     DGVECINMARGGRSSKSFIAEGSWNKCLDLKPDYVLIQFGHNDQPGHSTERATDAQTT
                     YWQ"
BASE COUNT          389 a          697 c          670 g          419 t
ORIGIN      
        1 ggttgctccg aacccggcat gtgggcacgt aagctgccca actgaagaac cctcaccgtg
       61 acatgggcct ccgacgcgtg cgtggtgcgt tcgtagtagc agagcctaag tttttcgcaa
      121 tccagtgcat cacgcacggg ccggaggccc gtgtcacgat gagcttcacc acgttctgca
      181 atcgcggctc agccaccttc gttcatcttg ctcgcgggtt gagtggtgat tcgttcgagc
      241 ctctccgtcc gaagattggc gcagtccttc acgatcaacg cctcgccctg tgacgcctcg
      301 atccgcgtat ccagaaatct gatcccatcc gcgttcgtgc aggtgaaccc cttctcagcg
      361 cgcacgtgca cattgctaaa tgtaatatcc gcgatcggct tctcgcgcag gcccgtgatg
      421 ctgcccgctt ctttcgcccc gcgggcggtg atgctgctga acaggaaatc gcggaacgtc
      481 ggcgtgcccg cgtccaccgg atagacgtcg ccgggtttgt ccttgcccgc gtagaacgtc
      541 gtgatcgtaa acggatgcgg cacatcctgc atgacgatgt tggaaacggc cacgccttcg
      601 acgactccgc cgcggccgcg ttgggatttg atgcggatgc cgttgtcggt gccgtggaag
      661 acgcagttgc tgacggtcac gttgcgcacg ccgcccgaca tttcgctgcc gatcgtgacc
      721 ccgccgtgac cgtgatagat gacgcagttg ctgatcgtga tgtcttcaca aggttttccc
      781 atgcggcggc cggcttcgtc gaggccggac ttcagcgtga tgctgtcgtc gccgttatcg
      841 acgcggcaat tactgatctg tacgttccgg cagctctcga cgttgatgcc atcggtgttc
      901 ggcgacggca ccggcgcgaa gatggtcaca ccgtcgatgc ggatgcgctc gcacaggagc
      961 gggtggatgt tccattccgg cgcgttttgc agcgtaacgt gctcgacgac gacatcgctg
     1021 cagcgcacga agcggatgag gcgcgggcga ccatattgcc aatcccctgg cgaagtcgca
     1081 gacgccaccg gctcggtggc gggagacgac gcggcacccg ccttgcgctt ggccttggcg
     1141 gccagaatcg gctcccacca cttgcgcccc tgcccatcaa tcacaccccg gccggtcacc
     1201 gcgacgcgct gcgcatcgac cgcataaatg agcggcccgc gcacctgccg gcccggtgtc
     1261 cacacttcat cgaccagcgg aaaatcatcc ggcgactcgc tcgccagcaa cgtcgccgcc
     1321 gcgtcgaggt cgagcgtcac gttgctcttc agcatgattg tgccggtgag atatcgcccc
     1381 ggcccgacgc gcaccgtacc cccaccggcc gcttcagccg ccgccaccgc ccgcgagaac
     1441 gcggcggtgt tgagcgtttt gccatcacca acgccgccga agtcgcggac gtcgaagacg
     1501 tccggcgctt cagcgccgct tgcgaagccg cgcgatgcga gcagcatcgc agtgagaatg
     1561 atcaacggaa atgcgcgatg tgatcggcgt tgggtggcgt tcatgtgtcg ttcgacctct
     1621 tccgcgcccc tgtgatttcg gccaatgtgg gtgactgact gtgatgagag tggccgcgcg
     1681 attagcgcgc gtcaatcgaa cggtccaatt tctaaaaaca ttgagcgggt tccgaaaagg
     1741 acgccgtcta tagtggcgac tccaatggtg ggcggagccg aggaagttca tcgatgaata
     1801 aaatgatcga cgtaattcgc gcagcggcgg ctgcgctcgc ggtgatggtt acctttgcaa
     1861 tgagcttgcg cgcggccgag cccgcggcgc cagccgatcg accggcgggc aagatccgca
     1921 tcgtcctcgc cggcgattcc accaccgcgg tcggcagcgg ttggggaccg ggcttcatca
     1981 aatgtctcga cgatggcgtc gagtgcatca acatggcccg cggcgggcgc agctccaaga
     2041 gcttcattgc cgagggatcg tggaataagt gcctggacct caagccggat tacgtgttga
     2101 tccaattcgg ccacaacgac cagcctggcc atagcaccga acgcgcgacc gatgcgcaga
     2161 caacctactg gcagt
//
LOCUS       DATIMH010000909         8356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5131975, whole genome shotgun sequence.
ACCESSION   DATIMH010000909 DATIMH010000000
VERSION     DATIMH010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5131975"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2131
                     /locus_tag="VLI90_15765"
     CDS             <1..2131
                     /locus_tag="VLI90_15765"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HSV15719.1"
                     /translation="TVLYDTFDVTDRLRAGDNAIGIILGNGMYNIQPSAGRYVKFTHS
                     FGPLKAIARLQLEYDDGSREVVATDSHWQAAPGPITYSNIYGGEDFDARRASARWDQP
                     DFSPGSHWEPAIESDGPGGALRGHSVSAPPIRAIEVEQPISANVVRPHVTVYDLGQNA
                     SHMPRIAVHGPAGSAVRVIPSELLGKDGLVDRASCVQDAGGPAWWQYTLAGGGQENYF
                     PKFFYQGCRYLQVECVAPEGSDQASQFPVVDSLEGVVVHSSSEPIGSFECSNALFNKT
                     YALVRWAQRSNMMSLMTDCPQREKLGWLEQLHLNGPSLRYNFRMDRLYAKEMNDMADA
                     QLPIGFVPNIAPEYFMAQSKVNEPFRNSPEWGSSFILAPWQQYLFTGDLSLFAVHYDQ
                     MVKYAHFLGTMAKADIIHTGLGDWYDLGPKPPWGSQLTPPAFTATAIYFYDLTVMARG
                     AELLGKSEDAAAFRARAADVRAAFNREFYNETRGDYATGSQCADAMPLALGIVEPENR
                     ASVLAALVADIELRGNALSAGDVGYRFVIRALADAGRSDVLFTMNNQSDRPGYGMQIR
                     KGATSLTEKWDASVGSFGSQDHFMLGQINEWFFHDLTGIQMDEQHPGFAHILIKPAVA
                     GDVSWVKASYQSDRGIIKVYWHAERDGVHLAITIPANTTATVFMPTHGGAGIIREING
                     AASNALPADGFSVGSGSYEFASRQNER"
     gene            complement(2382..5591)
                     /locus_tag="VLI90_15770"
     CDS             complement(2382..5591)
                     /locus_tag="VLI90_15770"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HSV15720.1"
                     /translation="MSRSKAARPQLETLEARVLFSVARPDHVVVVIEEDRFSNAIGDT
                     KDFPYLNQLAAGGLVYTNSHGVGHPSLPNYLALYAGSTLGVTDNGNTHTFTQPNLAKS
                     LNSLQVAPGQYLSFVGFAETLPQNGDTTTRIAPDPTISGSAPDVYMRNYNPMAQFADA
                     GTQNGVAVSNAAVNKTFASFPTTAAGFAALPNVAFVIPNTNHNTHGSNEQAPWATDPA
                     NYDMFRKNADAWLSAKMNAYAQWAKANNSILIITQDEEEDDYKIASTVSTLIVGDPRL
                     VVPGTNSTSVNHFNVTRTIEDMFGLPLLGSTGSVGDLTLDASGRLSPFPVTVTQSASN
                     TSLIASTASTVYGQSVTFTATVTGSGVPTGTVSFLDGSTVIGSGTVNASGVATFTTTA
                     LSGGATHSITASYGGDANSLASTSAAKSVTVSRASTTLALGASTSTTTFGQSVTLTAT
                     LSITSPGAGVASGSVSFSDGSMNLGSGTINAAGQATLAIATLGAGPHSITATYGGDTN
                     FSGSNSSSVGVTVAQSSTTTTLSVPSSAIVGQSVTFSATVAAVAPGAGSATGTVQFYV
                     DSVAFGSAVALSNGVATSPATTLALGGHAIVAVYSGDVNFAGSTSAQSTLNVSAAASS
                     TTIASSANPAPVGQPITFTATVTGTGGVPSGSVQFMLDGVAFGAAVALLNGSAVSDSI
                     STLAIGTHTVSAVYGGDANFAGSTSATLTQSVGTVNDAFANRMVLTGGVVSVIGSNIG
                     ATKEAGEPNHAANVGGASVWWTWTAPSTGTVVIDTAGSNFNTLLGVYTGTAVNALATV
                     ASNNDVVAGTLTSKVSFAITAGKVYQIAVDGFGGATGSIALNLNIIPTAPTGVSASDN
                     TFGDKIRVTWTAPANATAYEVWRNTSNKSSSAAKISTTGVVGTTFDDTTAATAKTYYY
                     WIKAKNAGGTSGFSAADTGIRAVAPLTNDAFANATALSGTSITVTGTNSGATKEAGEP
                     NHGGNTGGHSVWWSWTAPAAGTVTIDTLGSTFDTLLGVYTGSAVNALTTIASNDDSLS
                     GGTTTSKVTFTATAGTTYMIAVDGYGGVTGSITMHLSLV"
     gene            6287..7975
                     /locus_tag="VLI90_15775"
     CDS             6287..7975
                     /locus_tag="VLI90_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HSV15721.1"
                     /translation="MYPKPITLGIAIAFAVALFSLASHHASAQIASVDVSDPSNPEVS
                     YDPDPTVFAPDIPENVLQMYLPSPTDTANAGVSAVQKQNAVAVAAIALGASGVVTSTE
                     SYETAPYTAQEPTVTTSQPDTYTLDSGKKAAVYYIEPPQANGPYKQFDDLPVPCRFEF
                     GPASLPTDDGYIRITEQTTYGPLTGYGWSDTSQVVSGDNGGSNSTARGFCRMAHGRAG
                     GAIDQAVVSPTQFFVDVPNGTYKVSITSAPDKPGDTSRLSVWANGAPQFTLSTKSLTG
                     VSGTFTINVYDGRLMLEFFNSLDKVESATDPNTWVYVNGVTIERIPDDTPHKPTVFVA
                     SDSTAASYSPSIYPLTGWGARLQNFLTDDVVIEDDAVAGASSKSFYEHRWLKMIENRI
                     RPGDYFIVMFAINDSADDLPTQPATKRKTSPSSDFKAYLRQYVNAARDHGATPIFVTS
                     QTKRTYDAWGRFYNSVGAYPQAQAQLGAELKVPVIDLNTKSVNFLDAVGVDDSADIYM
                     FFPGGQYPGWPNGDADYIHLQDNGATQYAHLIVQGIREQQIQPLAQFVLDTPHP"
     gene            8055..>8356
                     /locus_tag="VLI90_15780"
     CDS             8055..>8356
                     /locus_tag="VLI90_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15722.1"
                     /translation="MSHLKLLDAITSTVITLIITAAMSHAATIYDSGGFEAPRFVAVP
                     TANNLAGQDPTPAGQGPWQQDAGTSTAVVQTNTPQAGLQAVEVQRLVGATGDTRWFV"
BASE COUNT         1505 a         2730 c         2592 g         1529 t
ORIGIN      
        1 gaccgttctt tacgacacgt tcgacgtgac cgatcgcctg cgcgccggcg acaatgcgat
       61 cggcatcatc ctcggcaacg gcatgtacaa catccagccc agcgccgggc ggtacgtgaa
      121 attcacccac tcgttcggcc cgctcaaggc gatcgcccgc ctgcagctgg aatacgacga
      181 tggctcgcgc gaggtggtgg cgacggattc gcactggcaa gcggcgccgg ggccgatcac
      241 atattccaat atctacggcg gcgaagattt tgatgcccgc agggcaagcg cgcggtggga
      301 tcaacccgac ttctcgcccg gcagccattg ggaaccggcg atagaaagcg acggacctgg
      361 cggcgcattg cgcggccata gcgtcagcgc gccgccgatc cgcgcgatcg aagtcgagca
      421 gcccatcagc gccaacgtcg ttcggccgca cgtgacggtt tacgatcttg ggcagaacgc
      481 gtcgcacatg ccgcggatcg ccgtgcacgg ccccgcgggt tcggcggtgc gcgtcattcc
      541 atcggagctg ctggggaaag acgggctggt cgatcgcgcc tcctgcgtgc aggacgccgg
      601 cgggcccgcg tggtggcagt acacgctggc cggcggcggg caggaaaact acttcccgaa
      661 attcttctac caggggtgcc gctatctgca ggtggagtgc gtcgcgccgg aaggatcaga
      721 tcaagccagc caattcccgg tcgtcgattc gctggaaggc gtggtcgtgc actcgtcttc
      781 cgagcccatc ggctcattcg aatgttcgaa cgcgctcttc aacaagacct acgcgctcgt
      841 gcgctgggcg cagcgcagca acatgatgag cctcatgacc gactgcccgc agcgcgagaa
      901 gctgggctgg ctggagcagc tgcatctgaa tggtccgtcg ctgcgctaca acttccgcat
      961 ggaccgcctg tacgccaagg aaatgaacga catggccgat gcgcagctgc cgatcggctt
     1021 cgtgccgaac atcgcgccgg aatatttcat ggcccagtca aaggtcaacg agccgttccg
     1081 caattcgccc gaatggggca gctcgttcat ccttgccccg tggcagcagt atctctttac
     1141 cggcgacctc tcgctgttcg ccgtccacta tgaccagatg gtgaagtatg cccatttcct
     1201 cggtacgatg gcgaaggccg acatcatcca caccggcctt ggcgactggt acgaccttgg
     1261 ccccaaaccg ccttggggtt cgcagctcac accgcccgcg ttcaccgcaa ccgcgattta
     1321 tttttacgat ctgaccgtga tggcgcgcgg cgcggagttg ctgggcaaat cggaggacgc
     1381 cgccgcgttt cgcgcgcgcg ctgcggatgt gcgcgcagca ttcaaccgcg agttctacaa
     1441 cgaaacccgc ggcgactacg ccaccggttc ccaatgcgcc gatgcgatgc cgctggcgct
     1501 gggcatcgtc gaaccggaaa atcgcgcctc cgtgctggcc gcgctcgtcg ccgatattga
     1561 acttcgcggc aacgcgctca gcgccggcga cgtcggctac cgcttcgtca tccgcgcgct
     1621 cgccgacgcc ggtcgcagcg atgtgctctt cacgatgaac aaccaaagcg accgccccgg
     1681 ctatgggatg cagatccgca aaggcgccac cagcctcacc gagaaatggg atgcatccgt
     1741 cggcagcttc ggttcacaag atcacttcat gctcggccag atcaacgaat ggttcttcca
     1801 cgacctgacc ggcattcaga tggacgagca acaccccggc ttcgcccaca tcctgatcaa
     1861 gcccgccgtg gcgggcgatg tgtcgtgggt gaaagcgtct tatcaatccg accgcggcat
     1921 catcaaggtg tattggcacg ccgaacgcga cggcgttcat ctcgccatca ccatccccgc
     1981 caacacgacc gcgacggtat tcatgcccac ccacggcggc gccgggatca ttcgagagat
     2041 caacggtgct gcttccaacg cgctacccgc cgacggattc tccgtcggct cgggctccta
     2101 cgagttcgcg tcgcggcaaa atgaaagatg agaacaccag acgtgtaacg atcgttcgcc
     2161 gccgcgctca cggctgcacg ggcgtcacgg tgttgtcgtt gtgatgtacg tcactgcttc
     2221 cgtagttgca gctgcattcg gagaagctcc ccactgctgt ggcttgtagc tgcgacacct
     2281 gcgtcgcctt gtttttccga gccgaaaggc gacgcaggcg tcgcagctac aagtattttt
     2341 ggaaagctct tacgagtttc ggtttcctcc tgcccacccg cctacacgag gctcaggtgc
     2401 atcgtgatgc tgccggtgac tccgccgtag ccgtcgaccg cgatcatgta agtcgtgccg
     2461 gcggtggcgg tgaaggtgac tttgcttgtc gtcgtgccgc cggagaggct gtcgtcattg
     2521 ctggcgatgg tggtcagggc gttgacggcc gagccggtat acacgccgag cagcgtgtcg
     2581 aaggtgctgc cgagcgtgtc gatggtgacg gtgccggcgg ccggcgcggt ccaggaccac
     2641 cagacgctgt gaccaccggt gttgccgccg tgattcggtt caccggcttc cttggtcgcg
     2701 ccggagttgg tgccggtgac ggtgatcgag gtgccggaga gcgcggtggc gttggcgaag
     2761 gcgtcgttgg tcagcggagc gacggcgcgg atgccggtgt cggcggcgct gaacccgctg
     2821 gtgccgccgg cgttcttggc tttgatccag tagtaatagg tcttggccgt cgcggcggtc
     2881 gtgtcgtcga aggtcgtgcc gacgacaccg gtcgtcgaga tcttcgccgc gctgctgctc
     2941 ttgttgctgg tgttgcgcca gacctcatag gcggtggcgt tcgcgggggc ggtccaggtg
     3001 acgcggatct tgtcgccgaa ggtgttgtcg ctggccgaca caccggtcgg cgcggtgggg
     3061 atgatgttga ggttcagcgc gatgctgccc gtcgccccgc cgaagccatc gacggcaatc
     3121 tgatacacct tccccgcggt gatggcgaag ctgactttgc tcgtgagcgt gcctgcgacg
     3181 acatcgttgt tcgacgcgac agtggcgagc gcgttgacgg cggtgcccgt gtagacgccg
     3241 agcagcgtgt taaagttgct gcctgcggtg tcgatgacga ccgtgccggt cgacggcgcg
     3301 gtccaggtcc accacaccga agccccgccg acgttggcgg cgtgattggg ttcacccgct
     3361 tctttggtcg cgccgatgtt agaaccgatg acgctgacaa caccgccggt gagaaccatg
     3421 cggttggcga acgcgtcgtt caccgtgccg accgattgcg tgagcgtggc cgacgtcgag
     3481 ccggcgaagt tcgcatcgcc gccatacacg gctgacacgg tgtgcgtgcc gatggcgagc
     3541 gtgctgatgc tgtcgctgac ggccgatccg ttcagcagcg cgacggccgc gccgaaggca
     3601 acgccatcaa gcatgaactg caccgagccg ctcggcacgc caccggtgcc ggttacggtc
     3661 gcggtgaagg tgatcggctg tccaaccggc gcggggttgg cgctgctggc gatggtcgtg
     3721 ctgctggcgg cggcgctgac gttcagcgtt gactgggctg atgtgctgcc ggcgaagttc
     3781 acgtcgccgg agtagacggc gacgatcgcg tgcccgccca acgcaagcgt ggtggccggg
     3841 ctcgtcgcaa cgccgttgct cagcgcgacg gcgctgccga aggcgacgct gtcaacatag
     3901 aactgcacgg tgccggttgc gctgcccgcg cccggcgcca ccgcggcgac ggtggcggag
     3961 aaagtaacgg actggccgac gatcgcgctg ctcggcacgc tcagcgtcgt ggtcgtcgaa
     4021 gactgagcga cggtcacgcc aactgaggat gaattgctgc cgctgaagtt cgtatcgccg
     4081 ccgtaggttg cggtgatcga gtgcggacca gcgcccagtg tcgcgatcgc cagcgtcgcc
     4141 tgccccgccg cgttgatcgt gccgctgcca aggttcatcg agccgtcgga gaacgaaacg
     4201 ctaccgctgg ccacgcccgc gccgggtgac gtgatgctga gcgtcgcggt gagcgtgacg
     4261 ctctggccga aggtggtggt cgacgttgaa gcgcccagcg ccaacgtcgt cgaagcgcga
     4321 gacaccgtca cgctcttggc ggccgacgtg ctggcgaggg agttcgcgtc accgccatag
     4381 ctggcggtga tcgaatgcgt cgcaccgccg gacagcgcgg tcgtcgtaaa tgttgccacg
     4441 cccgaagcgt tcaccgtgcc gctgccgatg acggtcgagc catcgaggaa cgagacggtg
     4501 ccggtcggaa cgccggagcc cgtgacggtc gcggtgaacg tgactgactg cccatagacg
     4561 gtggacgcgg tgctggcgat gagcgaggtg ttcgacgccg actgagtcac cgtcaccggg
     4621 aacggcgaca gccggcccga tgcgtccagc gtcaggtcac ccacgctgcc cgtgctgccc
     4681 agcagcggca ggccgaacat gtcttcgatt gtgcgcgtga cgttgaagtg attgacgctg
     4741 gtcgagttgg tgcccggcac aaccagccgc gggtcgccga cgatcaacgt cgatacggtg
     4801 gaagcgatct tgtaatcgtc ttcctcttcg tcctgcgtga tgatcaggat gctgttgttc
     4861 gccttggccc actgcgcgta agcgttcatc ttcgccgaaa gccaggcgtc tgcgttcttg
     4921 cggaacatgt catagttggc cgggtcggtg gcccaaggcg cttgttcgtt ggaaccgtgc
     4981 gtgttgtggt tcgtgttcgg gatgacgaac gcgacgttcg gcagcgccgc gaatcccgcg
     5041 gccgtcgtgg ggaagctggc gaaggtctta ttgaccgccg cgttcgacac ggccacgccg
     5101 ttctgcgtgc cggcgtcggc gaactgcgcc atcggattgt aattccgcat gtaaacgtcg
     5161 ggcgctgatc ccgagatggt cggatcgggc gcgatgcgcg tcgtcgtgtc accattctgc
     5221 ggcagcgtct cagcaaaccc gacgaaactc aggtactgcc ccggcgccac ctgcaatgag
     5281 ttgagcgact tagccaggtt cggctgcgtg aaggtgtgcg tgttgccgtt gtcggtgacg
     5341 ccaagcgtcg atccggcgta cagcgccaga taattgggca agctgggatg acccacaccg
     5401 tgcgaattgg tgtagaccaa gccccccgcc gccaactggt tcagataagg gaaatctttc
     5461 gtgtcaccga tcgcgttgga aaagcgatct tcctcgatca ccacgaccac gtgatccggc
     5521 cgggcgacgg aaaacagaac ccgggcttcc aacgtctcca actgcgggcg ggcggccttg
     5581 ctgcgcgaca tcttcaatcc cttccaaaat gagcgcaccc acagtcagcc ggtgaagcgc
     5641 gcgcacctcg ccatgggcga gaatgagacg gcgagcgcgc gccttcaacg agattcaaaa
     5701 accaactgat ggatgagcaa cgcgacggcc gaaacttcca acgacaccca cggccgcgcg
     5761 acgtaccatt gtagttaccc caagctaagc gcgttggcga accgacgcaa cgaatttccc
     5821 gccggtttgc tgtggataac ccgcacatcc ttcccaggtc accgcctcgc ccgcatagac
     5881 cgcggcgact gacgtggccg acgtttgatc cccggattcg ccacccgcgt tatccggcac
     5941 ggtcgcgcgc cgcgcgaaaa gacgaccatc gcagtaacgg ccgatttcag aaatcgtttg
     6001 tcgcggcacc cgcaacgtga tggtaattag tgaaagttct tccgaaagtt tttttgatct
     6061 ttctctgatt tttcgagttg tgcaaaatct gcaatcggcc ttactttgcc cgtgttcttt
     6121 gacatttcag tgcaacggca cttttccttc tgaagcgttg ttcttttgta cgggaggttt
     6181 cctgttgcat cagcggacca acatctaccg ctgattccca cacgcacgcg aatcgcacgc
     6241 agttcacgct tcctttcctt tgcgggggcc aaggagatgt acgcctatgt acccaaagcc
     6301 aatcacgctc ggcatcgcca tcgcgtttgc cgtcgcgctg ttctcgctcg cgtcgcacca
     6361 cgccagcgcg caaatcgcgt cggtcgatgt ttccgatccc agcaaccccg aagtgtcgta
     6421 cgaccctgat ccaaccgtct tcgcgccgga cattcccgag aacgtcctgc agatgtacct
     6481 gccctcgccc accgacaccg ccaacgcggg tgtgtcggcg gtgcagaagc agaacgccgt
     6541 tgccgtcgcc gccattgcgc tgggcgcatc cggcgtcgtc accagcaccg agtcctacga
     6601 gacggcgcct tacaccgcgc aggaaccaac ggtcacgaca tcgcagcccg acacctacac
     6661 gctcgactcc ggtaaaaagg ccgcggtcta ctacatcgag ccaccgcagg ccaacgggcc
     6721 ttacaagcag ttcgacgatc tgccggtccc ctgccgcttc gagttcggtc cggcatcgct
     6781 gccgaccgac gacggctaca tccgcatcac cgaacaaacc acctacggtc cgctgaccgg
     6841 ttacggctgg tccgacacct cccaagtcgt cagcggtgac aacggtggca gcaattccac
     6901 cgcacgcggt ttctgccgga tggcccacgg ccgcgccggt ggcgcgatcg accaggccgt
     6961 cgtctcgccc actcagttct tcgtcgatgt gcccaacggc acgtacaagg tgtcgatcac
     7021 ttccgcgccc gacaaaccgg gcgacaccag ccgcctgtcg gtctgggcca acggcgcgcc
     7081 gcagttcacg ctcagcacca agagcctgac cggtgtgagc ggcacgttca ccatcaacgt
     7141 ctacgatggc cggttgatgc tcgagttctt caactccctc gacaaagtcg aaagcgcgac
     7201 cgatcccaac acctgggtgt acgtcaacgg cgtgacgatc gagcgcattc ccgatgacac
     7261 gccgcacaag cccacggtct tcgtcgcctc cgattccacc gccgccagct acagcccgag
     7321 catttacccg ctgaccggtt ggggtgcgcg gctgcagaac ttcctcaccg atgatgtcgt
     7381 catcgaagac gatgccgtcg ccggcgccag ctctaagagc ttctacgaac accgctggtt
     7441 gaagatgatc gagaaccgca tccgacccgg tgattacttc atcgtgatgt tcgccatcaa
     7501 cgactcggcc gacgatctgc cgacgcaacc ggcgaccaag cgaaagacca gcccgagcag
     7561 cgacttcaag gcgtacctgc ggcaatacgt gaacgccgcc cgtgaccacg gcgccacgcc
     7621 gatcttcgtc acctcgcaga ccaaacgcac ctacgacgcg tggggccggt tctacaacag
     7681 cgtcggcgct tacccgcagg cgcaggccca actgggggcc gagctgaagg tccccgtcat
     7741 cgacctcaat accaagagcg tcaacttcct cgacgcggtc ggcgtcgatg actcggccga
     7801 catctacatg ttcttccccg gcgggcaata ccccggttgg ccgaacggcg atgccgatta
     7861 catccacctg caggacaacg gcgcgacaca gtacgcgcac ttgatcgtgc agggcatccg
     7921 cgagcagcag atccaaccgc tcgcccagtt cgtgctggat acgccacatc cataaccgac
     7981 gagtgaccca tcacgccccc gcgcacggca agcgcgcggg ggcgcggttc gagcgcaccc
     8041 tttggaggaa cgccatgtct catctgaagc tattggacgc aatcacctct accgtcatta
     8101 cgttgatcat caccgccgcc atgtcacacg ctgcgaccat ttacgacagc ggcggcttcg
     8161 aagcgcctcg cttcgttgcg gttcccactg ccaacaacct ggccggtcaa gatccgacgc
     8221 ccgcgggaca agggccctgg cagcaggatg ccggcaccag caccgccgtc gtgcagacca
     8281 acaccccgca ggccggcctg caggccgtcg aggtccagcg cctcgtcggc gccaccggtg
     8341 acacccgctg gttcgt
//
LOCUS       DATIMH010000910         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_217178, whole genome shotgun sequence.
ACCESSION   DATIMH010000910 DATIMH010000000
VERSION     DATIMH010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217178"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VLI90_15785"
     CDS             complement(<1..450)
                     /locus_tag="VLI90_15785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV15723.1"
                     /translation="MAIDFLIPTFNEETNLPFAIESIQGLARRIFVIDSGSTDRTKEI
                     AEEMGATFVHHPWEGYARQKNWALDNLPWESEWVFILDADEAVTPDLKDELQRVCDVP
                     PDTVPHAGFYVNRYFVFMGKIIRHCGYFPSWNLRLFKRGAARYEDRAI"
     gene            588..1154
                     /gene="hpt"
                     /locus_tag="VLI90_15790"
     CDS             588..1154
                     /gene="hpt"
                     /locus_tag="VLI90_15790"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HSV15724.1"
                     /translation="MQGDIERVLISQDRIARRVRELAEQITADHTPPRMPDGGEEITI
                     VPILTGAFIFCGDLIRQIPLPIQIGLLTVSSYPGSSLRTQGSQLLGKQLGDVRGHHVL
                     LIDDILDSGGTIKLVVPLLKELGAASVKSCVLLRKDRPAAKETVVDYVGFEIPDEFVV
                     GYGLDYNNYYRNLPDIVTLRAEAIERKA"
     gene            1225..1815
                     /locus_tag="VLI90_15795"
     CDS             1225..1815
                     /locus_tag="VLI90_15795"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSV15725.1"
                     /translation="MVYPPACASEGAAAAAAARPASATSLATLLTSHILRDGELVLLI
                     LKPSIWFILLSSLRFVALALILIIGAKVFNQHLAGPYRGYAEIGATLIVGRLMWGTLV
                     WMGRLYILTDLRILSISGVFNVEVFDCPLRKVARTRLMRGVEDRIVGVGSIEIIPQDE
                     SYPFGLWQTVGRPKQVHQQIVATINRAKQGSGMPCE"
     gene            complement(1886..>2297)
                     /locus_tag="VLI90_15800"
     CDS             complement(1886..>2297)
                     /locus_tag="VLI90_15800"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSV15726.1"
                     /translation="ASGQASVVYCPRTHAYFGHPPHRWRDMLAAGINVAVGTDSCASS
                     PDLNLVDDLRLLHRIAPDVPAQTLWEMATIRGARAIAQQSLVGSIEVAKAADLTIFLV
                     QSDDPLTEILESSALPAEVWIAGRRISHATMERS"
BASE COUNT          426 a          713 c          728 g          430 t
ORIGIN      
        1 gatcgcgcgg tcctcgtatc gcgcagcgcc gcgcttgaac aaccgcaggt tccagctggg
       61 aaaatatccg cagtggcgga tgatcttgcc catgaacacg aagtaccgat tgacgtaaaa
      121 ccccgcgtgc ggcacggtgt ccggcgggac atcgcacacg cgctgcaact cgtccttcag
      181 atccggcgtc accgcttcat cggcgtcgag gatgaagacc cattcgcttt cccacggcag
      241 gttgtccagc gcccagttct tctgccgggc gtagccctcc caggggtgat ggacgaacgt
      301 cgcgcccatc tcctcggcga tttctttcgt gcgatcggtg ctgccggaat cgattacgaa
      361 aatccggcgg gccagaccct ggatgctttc aatggcaaaa gggaggttgg tttcctcgtt
      421 gaaagtcggg atcaggaaat cgatggccat gcgtttgggg attcgaagta tagcatgggt
      481 ggcggcgcgt tgtagctgcg acgcccgcgt cgcccggcac atttgctggc gcgcgatcgt
      541 ccgataatcg gttctctccg ccgcacggtt cggatagatt gagccggatg cagggtgaca
      601 tcgaacgcgt gctcatttcg caggaccgga ttgcccgccg cgtgcgcgag ctggcggagc
      661 agatcacggc cgaccacaca ccgccgcgga tgccggatgg cggcgaggaa atcacgattg
      721 tgccgatcct caccggcgcg ttcatcttct gcggcgactt gatccggcag atcccgctgc
      781 cgatccagat agggctgctc accgtcagca gctacccggg ttcaagcctg cgcacgcagg
      841 gatcgcaatt gctcggcaaa caactgggcg acgtgcgcgg gcatcacgtg ctgctgatcg
      901 acgacatcct cgacagcggc gggacgatca agctggtcgt accgctgctg aaggaactcg
      961 gggccgccag cgtgaagtcc tgcgtgctgc tgcgtaaaga tcgccccgcg gcgaaggaga
     1021 cggtggtgga ttacgtgggg ttcgagattc ccgacgaatt cgtcgtgggg tacggacttg
     1081 attacaataa ctattatcgc aatttgccgg acatcgtcac gctaagggct gaggcgatcg
     1141 agcgaaaggc gtgataaccg cttgtggatt ggcatctttt caacttcgca ggaaacacgc
     1201 tctaagccgc aagcggcgga gcgcgtggtc tatccaccgg cgtgcgccag cgaaggcgcc
     1261 gccgccgccg ccgcggcgcg acctgcgagt gccacgtcgc tggcgacgct gctcacatcg
     1321 cacatcctgc gcgatggcga gctggtcctg ctcatcctca agccgtcgat ctggttcatc
     1381 ctgctcagca gcctgcgctt cgtcgcgctg gcgctgatcc tcatcatcgg cgccaaggtc
     1441 ttcaaccagc acctggccgg gccttatcgc ggctacgcgg agatcggcgc gacgctgatc
     1501 gtcggccggt tgatgtgggg cacgctggtg tggatgggcc ggctgtacat cctgaccgat
     1561 cttcgcatcc tcagcatcag cggcgtgttc aacgtcgaag tcttcgactg cccgctgcga
     1621 aaggtcgcgc gcacccgcct gatgcgcggc gtcgaagacc gcatcgtcgg cgtgggctcc
     1681 attgaaatta tcccgcagga tgagtcgtat cccttcggtc tctggcaaac ggtgggaaga
     1741 cccaagcagg tgcaccagca gatcgtcgcg acgatcaacc gcgccaagca gggcagcggg
     1801 atgccgtgcg agtaaacgca ccttgatagc cgggggcgga agccggtctt ttcgaacgta
     1861 aaaagaccgg agcttccgct tccggctatg accgctccat cgtcgcgtgg ctaattcggc
     1921 gcccggcaat ccacacctca gctggcaacg ctgacgattc aagaatctcc gtcaacggat
     1981 catcactttg cacgaggaag atcgtcaagt ccgccgcctt agcgacctcg attgaaccga
     2041 ccaaagattg ttgggcgatc gcgcgagcgc cgcgaatcgt cgccatctcc caaagcgtct
     2101 gcgccggcac gtctggcgcg atgcggtgca acagccgcag gtcatcgacg agattcagat
     2161 ccggcgagct ggcgcagctg tcggtgccaa cggcgacgtt gatgccggcc gccagcatgt
     2221 cgcgccagcg atgcggcggg tggccgaaat acgcgtgcgt ccgtgggcag tacacgacgc
     2281 tggcctggcc gctggcc
//
LOCUS       DATIMH010000911         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3106920, whole genome shotgun sequence.
ACCESSION   DATIMH010000911 DATIMH010000000
VERSION     DATIMH010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3106920"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1115
                     /gene="truA"
                     /locus_tag="VLI90_15805"
     CDS             318..1115
                     /gene="truA"
                     /locus_tag="VLI90_15805"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HSV15727.1"
                     /translation="MAIQRYKLTIAYRGTQYHGWQQQAPTITWKGPVPPAGHGIPTIQ
                     ESLRRALTSVVRHDVIISGSSRTDAGVHAKGQIAHFDTDKTQIPIEALRQSANARLPD
                     DILIRAIEPVPETFNAIGSTISKRYQYVIWHATDRPPFFADLVWHRWHPLDIDAMRAA
                     AAMFEGEHDFASFARPGHGREHTIRTVHACTLAYRQPRLVIGVEGSGFLWQQIRIMLG
                     TLVEVGLGRYGPETIRHMLEAKDRQAAGPTAPPQGLYLQWVRTSESR"
     gene            1276..1821
                     /locus_tag="VLI90_15810"
     CDS             1276..1821
                     /locus_tag="VLI90_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15728.1"
                     /translation="MTPRLDALVIALAATHFAAGVAMLSLAVTRGLTAATATRVSASA
                     SFVALPGLLLLIAGALTLIRHPLAWRITTIALEIAAALLIMLLGFSTLMPMAAGVMLG
                     DDEPLFSYMLLGVALAAPAALAFLPAAIEFLLLSNPAVQAAYQVSTAQPNPRWWRRPL
                     RHLAIFTAAGLVAAMASFQII"
     gene            1829..2236
                     /locus_tag="VLI90_15815"
     CDS             1829..2236
                     /locus_tag="VLI90_15815"
                     /inference="COORDINATES: protein motif:HMM:NF025183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HSV15729.1"
                     /translation="MRDDLDELFAALARSTFRSRFQLRGKELDYLQTKGLPLVLEHAA
                     DLIANRLAPASPENDGQQTPFRNHPVFVAQHATATCCRGCLEKWHHIPRGRALSEAEQ
                     RYVVSVIGRWLFRFAKPQAAAEPQLFDPQTGEV"
     gene            2581..3662
                     /gene="purF"
                     /locus_tag="VLI90_15820"
                     /pseudo
     CDS             2581..3662
                     /gene="purF"
                     /locus_tag="VLI90_15820"
                     /inference="COORDINATES: protein motif:HMM:NF024927.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
BASE COUNT          651 a         1097 c         1214 g          700 t
ORIGIN      
        1 cgcgatggtg gtcagcgagt gacggtgaag tttcatggtt gctgataccg tacgggctgt
       61 ggtgtgttgt taccactcgg ggcggagcaa tggcgtgtct gggaatggcg aattccgaaa
      121 cccgaatccc cgaatggatg acgaatgacg aaatccgaat gacgaaacgg cgcgatcgaa
      181 agccattcgc cattcgccat tcgtcattcg gatttgattc ggggattcgg gtttcggaat
      241 tcggatttcc ggtttcggct tcccccgtgc ctcgcagcgt tcccccgctt ggcgctttcg
      301 ggtatcacca ccgccgcatg gccattcagc gttataagct gacgatcgcg taccgcggaa
      361 cgcagtatca cggctggcag cagcaggcgc cgacgatcac ctggaaaggc ccggtgccgc
      421 cggcggggca cgggattccg acaatccagg agagccttcg ccgcgcgctg acgagcgtcg
      481 tccggcacga cgtgatcatc tccggctcat cgcgcaccga cgccggggtg cacgccaagg
      541 ggcagatcgc ccatttcgac acagacaaaa cgcagatccc catcgaagcg ctgcggcaat
      601 cagccaacgc gcgcttgccg gacgacattt tgatccgcgc catcgagccg gtgccggaga
      661 cgttcaacgc gatcggttcg acgatcagca agcgttatca gtacgtgatc tggcacgcga
      721 ccgatcggcc gccatttttc gccgatctgg tgtggcaccg ctggcacccg ttggacatcg
      781 acgcgatgcg cgcggcggcg gcgatgttcg aaggcgaaca tgatttcgcc agcttcgccc
      841 gcccggggca tggccgcgag cacacgatcc gcacggtgca tgcgtgcacg ctcgcgtacc
      901 gccagccgcg actggtcatc ggcgtcgaag gcagcgggtt tctgtggcaa cagattcgca
      961 tcatgctggg gacgttggtc gaggtggggc tggggcgata cgggccggag acgatccggc
     1021 acatgctgga ggcgaaggac cggcaagccg ccgggccgac cgcgccaccg caggggttgt
     1081 atttgcagtg ggtgcgcacg agtgagagcc gatgagcggc gttgtcgctt tagcgaccat
     1141 ggctttttga atcgaagacg tggtcgccga agcgaccacg ccgtgcgggc tttttccggc
     1201 acacgaattg ccaccttttt gggcggagaa ccgcccatgg ctgcagcctc gaccgcacat
     1261 gttgtacggc cgtctttgac accgcgtctg gacgcgctgg tcatcgcgct ggcggccacg
     1321 cattttgcgg cgggggttgc gatgttgtcg ctggcggtaa cgcgcggcct cacggccgcg
     1381 accgcgacgc gcgtttccgc cagcgcgtcg ttcgtcgcgc tgccggggct gcttctgctg
     1441 atcgccggcg cgctcacgct cattcgacat cccctggcgt ggcgcatcac gacgatcgcg
     1501 ctggagatcg cggcggcgct gctgatcatg ctgcttggct tcagcacact catgccgatg
     1561 gccgcggggg tcatgctcgg cgatgacgag ccgctgtttt cgtacatgct gctgggcgtc
     1621 gcgctggcgg cgccggcggc gctggcgttt ttgccggcgg caatcgagtt tctgctcctg
     1681 agcaatccgg cggtgcaggc ggcatatcag gtttcaacgg cgcagccgaa tccgcgctgg
     1741 tggcgacggc cgctgcggca tcttgcgatc ttcaccgcgg cggggctcgt cgccgccatg
     1801 gcttcgttcc agatcatata atcgggcgat gcgcgacgat ctcgatgagc tgttcgctgc
     1861 gctggcacgt tcgaccttcc gcagccgatt tcaactgcgc ggcaaagaac tggattacct
     1921 gcagacgaag ggattgccgc tggtgctcga gcacgcggcg gatttgatcg ccaaccgact
     1981 ggcgccggct agtcctgaaa acgatggcca gcaaacgcca tttcgcaacc atccggtgtt
     2041 cgtggcccag cacgcgacgg cgacgtgctg ccggggatgc ttggagaagt ggcaccacat
     2101 tccgcgcggc cgggcgctga gcgaggcgga gcagcggtat gtcgtgagcg tgatcgggcg
     2161 gtggttgttc cgatttgcca agccgcaagc ggcggcagaa ccacagctat ttgatcctca
     2221 gaccggggaa gtttgacgac cttggacatg ccccgtaagc ttcggacttg atgctggttc
     2281 gtctggacga tcagcagcat tcctcaacgc ggttcgcgcg gcgcagacaa ttgaactttt
     2341 gcaaaattcg gagatgcgct cttggctgtt tcgcttgaat tgttcgacga ttacgagaag
     2401 aaggaaaagt gcggcctgtt tggcgtgtgg ggcacgcccg acgcgtcgca gatcgtctat
     2461 cagggcctgt tcgcgcagca acatcgcggg caggaatcgg ccggtattgc cgtctctaat
     2521 ggctcggaac tgaacggcca caccggcatg gggctggtca gccaatgttt caacccgcgg
     2581 atgctgcggg aagatctggc ggggcacgcg gccatcggtc acgtgcgata ttcgaccacc
     2641 gggtcgagca agctgtgcaa cgcgcagccg ctgctgcggc aattttccct cgggcctgtt
     2701 gcggtcgcgc ataacggaaa cctgatcaac gcggcgttgc ttcgcagcga gtacgaacgg
     2761 cacgggcaca tcttccagtc tacgaccgac accgagatca tcgttcacct gctggcctag
     2821 ccgacgcatt tggagaagca ggatccgctg ccgcacgtgc tgaagcattt gcagggcgcg
     2881 ttcagcctgc tgtttttgtt cccggatcga atggaagcgg tgcgcgatcc gtggggcgtt
     2941 cgcccgctgg cgctggggaa aaccgcgcag gggcaatggt gcgtggccag cgaaacatgc
     3001 gctttcgacg cgatcgatgc gcaatacatc cgcgagatcg agcccggcga gatcgtgcgc
     3061 atcgacgaca gcggcatctc atcgcgcaag tacgatgcgc cggcccgcga gatggcgcac
     3121 tgcgtcttcg agcacgtgta cttttcgaat cccgccagca agattttcgg ccagacggtg
     3181 catgttgtgc gcgagcggat gggccggcag ttggcgaagg aatcgccggt cgatgccgac
     3241 tacatcatgc cgatgcccga cagcgggcgc agtgcagcac tggggttcgc caaggaatcg
     3301 cagatcgtct tcgaagaagg gatcgtgccg aaccgcttcg tcggccgaac gttcatactc
     3361 ccgcaacagg gccagcgcga ccgcgcggtg gcgatgaagc tgaacattat tcccgacgtg
     3421 gtcgatggca agcgcatcgt gatcgtcgaa gattcagtcg tgcgcggcac gacgacgcgc
     3481 agcaagatgc gggcgatccg ccgggccggg gcgaaggaaa ttcacctgcg ggtgagctgc
     3541 ccgccgatcc ggcatccttg tttttacggc atcgattttc cgacgagtgc tgagctgatc
     3601 gcgcacaacc gcagcgtcga gcagatcgcg gagttcctgg aggtcgacag cctggcgtat
     3661 ct
//
LOCUS       DATIMH010000912         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5132658, whole genome shotgun sequence.
ACCESSION   DATIMH010000912 DATIMH010000000
VERSION     DATIMH010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5132658"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VLI90_15825"
     CDS             <1..229
                     /locus_tag="VLI90_15825"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HSV15730.1"
                     /translation="LAGTLVAIIILVVGSLLIRVYCELVILVFKIYDELKAIRTGTPP
                     PDQGFPVTPMSPMPITPGAPGDTLPPTSAMA"
     gene            complement(307..774)
                     /locus_tag="VLI90_15830"
     CDS             complement(307..774)
                     /locus_tag="VLI90_15830"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSV15731.1"
                     /translation="MVYPQAYHITFSVYLARPPGSPKPHVDRDHNDYGKPLAPTNEGR
                     EQWPRENASESPIELTIEQRRLVEEAIRDLAARYRWLIHAIAVMRDHVHVVISAARDG
                     EQLRDAIKAVASKWLNKQSGKRTWWAEGGSAKYLWERPYFVNATKYVNDQREL"
     gene            977..2146
                     /gene="aroC"
                     /locus_tag="VLI90_15835"
     CDS             977..2146
                     /gene="aroC"
                     /locus_tag="VLI90_15835"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HSV15732.1"
                     /translation="MATLSYRTAGESHGKALIALVEGMPAGVPLDKEFIDGELRRRQG
                     GYGRGGRQKIETDQIDFLAGVRMGKSIGSPITMLIPNKDSRLDDPSATPPLHRPRPGH
                     ADLAGSIKWLTTDCRETLERASARETAARVAAGGLARCFLRQFSIEVFGFVRSVGEAT
                     AKVEVTADNWRELAKLRDASDVYCPDPAADAAMKDFINQQKMAKDTAGGVVETHVFGC
                     PVGIGSCMTWDGRLDSRIAAAVVGIQAFKGVEIGLGFETARRPGSKVHDEIAFDPSKR
                     DTPSLGFTRSTNSAGGLEGGMTNGQPVVVRGAMKPISTLGKPLKSVDLNTKQPSEAGW
                     ERSDISAISAAGVVMENVIAFEIARAFLEKFGGDSITEVRANYDNFLATARRLPL"
     gene            complement(2313..3539)
                     /locus_tag="VLI90_15840"
     CDS             complement(2313..3539)
                     /locus_tag="VLI90_15840"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HSV15733.1"
                     /translation="MIRRNRLNPPRPKSNRRRAGKPRVLLAMGLQNPQRQAGIVRYAR
                     SAGWIVDSRLLSYHARGQDREYFQSARFDGVLALCSLAAPWILDLVKQFKVPVVDMWA
                     DYPAERFPRVLLDHATIGEIGATYLLGRGFRNLLFYTHAIEGKVGSVRREAFRKAVAA
                     EPGATFHALTWDPAAPPAGAPSPITWLAKWLAHAPLPLAVMGSNDYIACEVLEAAALA
                     GLRVPAQVAVLGVDNDPLVTELAPVPLSSIDSARERVGYEAAALLERLMRGERPPSEP
                     LRIAPSRVVTRLSTDVLAVDDPEVAGAMRYILANFRRGISVDDVAAQAHVSRRHLQDR
                     FLNKTGRTIVEMITLQRLDRAKQLLSETDAKIHTVAQQCGFSTGERMSKVFRRLEGLT
                     PQEFRRSYGRIESKSE"
     gene            3741..>3989
                     /locus_tag="VLI90_15845"
     CDS             3741..>3989
                     /locus_tag="VLI90_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15734.1"
                     /translation="MTSNHSKCGVVLGTFLFMLLTSAPRSLAATGAFEPPASPRAAID
                     FNAGWRFIREDASGADAPAFDDSRWEQVTTPHTFNDVDS"
BASE COUNT          716 a         1360 c         1232 g          681 t
ORIGIN      
        1 cctcgccggc acgctcgtcg ccatcatcat actcgtcgtc ggctccctgc tcatccgcgt
       61 ctattgcgag ctggtcatcc tcgtcttcaa gatctacgac gaactaaaag ccatccgcac
      121 cggcacgccc ccgccggatc agggctttcc ggtcacccca atgtccccga tgccgataac
      181 gccgggtgcg cccggcgaca cgctgccgcc gacgtcggcg atggcgtgat gttctaaggg
      241 tgcctccgaa cctgcgccag cacagagccc agcggctccg ccgcggggcg cgcgggaggc
      301 gcgcagtcac agttcccgct gatcattcac atacttcgtc gcgttcacga aatacggccg
      361 ctcccaaaga tacttcgcgc ttccgccctc ggcccaccag gtgcgcttcc cggactgctt
      421 attcagccat ttactagcga cagctttaat cgcatcacgg agctgttccc cgtcccgggc
      481 tgcgctgatg acgacgtgga catgatcgcg catgactgca atcgcgtgga tgagccagcg
      541 gtatctcgcg gcaagatcgc gtatcgcctc ttcaaccagc cttcgttgtt caatcgtgag
      601 ttcgatcgga ctctcgctcg cgttctcacg cggccactgc tcacgcccct cattcgtcgg
      661 cgcgagtggt tttccatagt cattgtggtc ccggtcaaca tgcggcttgg gagaacccgg
      721 cggacgagcg agataaacag aaaaagtgat gtggtaagcc tgagggtaga ccatacatcg
      781 cattctaatg ggtgccacga ctttgcgcca gcagagagcc cagcggctcc gccgcgggac
      841 gggagtcgat ttgcgtcact tgcgaaggat gcgcaactct ccccccgccc cgcggcagaa
      901 ccgctgggct ctctgctccc gcagcgacaa taccgctttt cgcgccggcg gattcctctg
      961 ctacaatcac ccaggaatgg ccactctgtc ctaccgcacc gcgggtgaat cgcacggcaa
     1021 ggcgctcatc gcgctcgtcg aaggcatgcc tgcgggcgtg ccgctcgata aggaattcat
     1081 cgacggcgaa cttcgccgcc gccagggcgg ctacggccgc ggggggcggc agaagatcga
     1141 gaccgaccag atcgatttcc tcgccggcgt gcgcatgggc aagtcgatcg gctcgccgat
     1201 cacaatgctc atccccaaca aggactcgcg gctggacgat cccagcgcca cgccgccgct
     1261 gcaccgcccg cggccgggac atgcggacct ggccggttcg atcaagtggt tgacgaccga
     1321 ttgccgcgag acactcgagc gcgccagcgc gcgcgagacc gccgcgcgcg tcgccgcggg
     1381 tggactggct cgatgcttcc tccgccagtt cagcattgag gtgttcggct tcgtccgcag
     1441 cgtcggcgaa gcgacggcga aggtggaagt gacggccgac aactggcgcg agctggcgaa
     1501 gctgcgcgac gcgtccgacg tctactgccc cgaccccgcg gccgacgccg cgatgaagga
     1561 cttcatcaac cagcagaaga tggccaagga caccgccggc ggcgtcgtcg agacgcacgt
     1621 ctttggctgc ccggtcggca tcggttcgtg catgacgtgg gacggccggc tcgacagccg
     1681 catcgccgcc gccgtcgtcg gcatccaggc gttcaagggc gtggagatcg gcctcggttt
     1741 cgaaaccgcc cggaggccgg gttcaaaagt tcatgatgaa atcgcgttcg acccgtcgaa
     1801 gcgcgacacg ccgagccttg gcttcacgcg cagcaccaac agcgccggcg gcctcgaagg
     1861 cgggatgacc aacggccaac cggtcgtcgt ccgcggcgcg atgaagccca tcagcaccct
     1921 cggcaagccg ctgaagagcg tcgacctgaa caccaagcag cccagcgaag ctggctggga
     1981 acgcagcgac atcagcgcca tcagcgccgc cggtgtcgtg atggaaaacg tgatcgcctt
     2041 cgagatcgcg cgagccttcc tggaaaaatt cggcggcgac tcgatcacgg aagtccgcgc
     2101 caactacgac aacttcctgg ccaccgcgcg gcggctgccg ctgtagaagt cacacgaagg
     2161 gttttttgaa agttcgagaa ccccagcgtg acacgggcct ccgggcccgt gcgtgatgcg
     2221 ctgaatttca gaaaacgacg ggtcctgcta ctaccaaagc atcgagcacg ggccggaggc
     2281 ccgtgtcacg attgagcttt tcaaaaccgc ttctactcgc tcttcgactc aatccgccca
     2341 tacgacctgc gaaactcctg cggggtcagc ccctccagcc ggcgaaagac cttgctcatc
     2401 cgctcgcccg tgctgaagcc gcactgctgc gccaccgtgt gaatcttcgc gtccgtctcg
     2461 ctcagcaatt gcttcgcccg atcgagccgc tgtagggtga tcatctccac gatcgtccgc
     2521 cccgtcttgt tcaggaaccg gtcctgcagg tgacgccgcg agacgtgtgc ctgggcggcc
     2581 acgtcatcca ccgagatccc gcggcggaag ttggcgagga tgtagcgcat cgcgccggcg
     2641 acttccggat catccaccgc cagcacgtcg gtggacaatc gcgtgaccac ccggctgggg
     2701 gcgatgcgta gcggctcgct gggcgggcgc tcgccgcgca tcagtcgttc gagcaacgcg
     2761 gccgcttcgt agccgacgcg ctcgcgcgcg gagtcgatgc tcgacaacgg caccggcgcc
     2821 aactcggtca ccagcgggtc gttgtccacg ccaagcaccg ccacctgcgc cggcacgcgc
     2881 aggccggcca gcgcggcagc ctccagcact tcgcaggcga tgtaatcgtt cgatcccatc
     2941 accgccagcg gcagcggggc atgagcgagc cacttcgcca gccaggtgat cggcgacggc
     3001 gcccccgccg gcggtgcggc ggggtcccac gtcagtgcgt ggaatgtcgc gcccggctcc
     3061 gccgcgacgg ctttgcgaaa tgcttcgcga cggacgctgc ccactttgcc ttcaatcgcg
     3121 tgtgtgtaga acagcaggtt gcgaaatccg cggccgagca aatatgtcgc gccgatctcg
     3181 ccgatcgtgg cgtgatcgag cagcacgcgc gggaagcgct cggccggata atccgcccac
     3241 atatcgacga ccggcacctt gaactgtttg accaggtcca ggatccacgg cgccgccagc
     3301 gagcagagcg cgagcacacc gtcgaagcgg gccgactgaa aatactcgcg gtcctgcccg
     3361 cgcgcgtggt acgacagcag gcgcgaatcg acgatccacc cagcgctgcg ggcgtagcgg
     3421 acgatccccg cctgccgctg cgggttttgc aggcccatcg ccagcagcac gcgcggcttg
     3481 cccgcccgcc ggcggttcga tttcggtcgt ggtgggttga gacggttgcg tcttatcatc
     3541 tccatcggag aagataccgc caaaatagcg gtctgccgag accggcaagt aaatcgcctg
     3601 aatcggcagg cggtcgagcg tactgcacct tatgctgccg atgaggcaac gggtggtatc
     3661 tccgccgctt gcggcttagc gtccttttgc ttttggccga gaacagatgc taagccgcaa
     3721 gcggctcaat tgaacacgat atgacatcga atcattccaa atgcggcgtt gttctgggca
     3781 cgtttttgtt catgctgctg acgagcgcgc cgcgttcctt ggccgcgacc ggcgcattcg
     3841 aacccccggc gtcgccgcgc gccgcgatcg acttcaacgc cggctggcgg ttcattcgtg
     3901 aagacgccag cggcgccgac gcccccgcgt tcgatgattc gaggtgggag caggtcacca
     3961 cgccgcacac gttcaacgat gtggactca
//
LOCUS       DATIMH010000913        11371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2528195, whole genome shotgun sequence.
ACCESSION   DATIMH010000913 DATIMH010000000
VERSION     DATIMH010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11371
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2528195"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="VLI90_15850"
     CDS             complement(<1..1980)
                     /locus_tag="VLI90_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15735.1"
                     /translation="MAVAAPQASWTPLELLKHRFIANVDALAARYPQLAQRLRELSPS
                     QTYFIQAYPDHVRLGAGSIGAVNALPHPLLPVAALDLAKRLFSDDNYDQPVLVSGEDL
                     GWLWNRLYALPCNVPTAPGYRPPMHCLIRDVERLWVILHLQDWRQLLADARVRLFAGD
                     DAFDQFRRSLVHDSQCPWPKLSVTIDPALWPAGQTIDTVLAAARAAMTERLTQLNQRL
                     RLMYAGATPASIASLFQSGRPLRILGVTSRYTTFLQHSMRDWLAAFDRLGHETRLFIE
                     PADHEVPNNFSVAQACADFHPDLAVIIDHYRTEMGGFPDNLPVVMWVQDALSNLFTPK
                     AGAAQGERDYALGFAHLKMTREFGYPAARYMPAIVAVNEQRFAPQALTPSERDAFTCD
                     VSFVSNASTPAESILQAEITRLDTPQATRLLTVIFEQIKSIYDAGGSVTEPITIRRIV
                     DAAMIDTQTTLPPEQMPALMDLFTQRINNALFRHQSLEWLAGLGVNVHLYGRGWESHP
                     TLRRFARGVADNVHQLSKIYQASRINLQISPHGAVHQRVLEGLCTSGFFLFRHCPGDV
                     LEREFEAIWRWCQSQRITSDDQLRQRATPEIARRLARVAELLQLDPFAGDHPFIDVLH
                     SSQAAGYIRSAGSVWVDDYDAVSFRSNQELRAKVTH"
     gene            complement(1995..3584)
                     /locus_tag="VLI90_15855"
     CDS             complement(1995..3584)
                     /locus_tag="VLI90_15855"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSV15736.1"
                     /translation="MSNTAHRKICSLPQLLEFRRSATAAGKTIVHCHGCFDIVHPGHI
                     HYLQFARSLGDALIVTVSADPQVNKGINRPLIPDDLRAASLAALECVDCVYINPHPTA
                     VEVLDALKPDIYVKGREYERNEDPRFLAERDTVTRNGGRVVFSSGDVIYSSTALIGAM
                     DATATLFNDQKVGRFRQQFDLSAAALSNLVLRCRGKRMVVVGDYICDRYHFCDATGVA
                     SEGPMMTLRPLQSEQYDGAAAIIARHLAGLGADATLITSLADDDASRQVVARLTEAGV
                     TVRALRQRKQLVSKSRYLVDQTKLLKVDEGATTPLDSRSVDEAAWLIEEAATDAAGVI
                     FADFGYGLITGPLLDRVMPIIRPRVPLLTADVSGRQSTLLRFKDVDLLCPTEREVRQT
                     LNNFSSGLNAVVYELLATTAAKTAMITLGKQGLCLFDQQQQTNALSDSWDRKLRTAYL
                     PAMASHPVDPLGCGDALLAIASLALAAGGSPQAAAYLGSLAAAIEVQQIGNQPVTAEQ
                     LLDRINPSALQAHYEPARLAS"
     gene            complement(3743..4705)
                     /locus_tag="VLI90_15860"
     CDS             complement(3743..4705)
                     /locus_tag="VLI90_15860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV15737.1"
                     /translation="MPSDQGEKFIRTFAGTDNSCMRCWCGNERLIEWGEGYHRCDACQ
                     TLVAAITDRQADPRVIDDRADLYGRDYWFDHQTRELNCPDIITRSRTDLSDRCIHWLR
                     TLLQFKLPPARVLEIGCAHGGFVAMLHQSGFDATGLELSPSIVEYARQTFDVPVLSGP
                     IEDQTIAAGTLDAVILMDVMEHLPQPQATLRRCLDLLKSDGVLLIQTPSYPEGATLPE
                     LRAIGHKFSMMLDPNEHLFLFSPTAARKLFQQLDAEHIEFVPAIFGFYDMSFVVSRQS
                     IARTTPSERDAALTATLPGRFMQAMLDLDERRLNLLRLYRERAA"
     gene            4754..5569
                     /locus_tag="VLI90_15865"
     CDS             4754..5569
                     /locus_tag="VLI90_15865"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HSV15738.1"
                     /translation="MIDSTMTLTEQTKRKTARQYDHSAFYPTVRPTDVFLCSYPKSGT
                     TWLGFLIAQVLKKDESEELDLKSFNKYVPDVNLQYTKRGSLAQHAALPDPRFLLCHAT
                     YDAKLPKVVYVTRDPRDVMVSYWHYQKFLKEDFNLSLADFLRSDDHWPCEWDEHVADW
                     LLPEPHGNVLRVGYEEIHQDAAGVLRRVLQFGGVKASEARITAAVECSRFENMRAAEE
                     RFGVNGKAGDEQERFVRKGRVGSWQDEMGYTELRIVEERYGRVMRQVGYEPLS"
     gene            5619..6287
                     /locus_tag="VLI90_15870"
     CDS             5619..6287
                     /locus_tag="VLI90_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15739.1"
                     /translation="MLWSTDPKLEFINYYGNSSLFALDILRRHGPLDQMKLFSIGCLM
                     PHLADYLALFHTVHHNSLVTTKLPMPPELFPAKFVEHQTDFFDLPPLDIDCAISHVAI
                     HCFNDTRYGNASSNDGWQKPYKAAAKLRQMAGPRKLPAIVSVAVNEQEGFYDNNVHLS
                     HARFIASFEAAGFKLREYFFDYVCGGTTYKPEYLEYEYRRSKKLPGHAESPRQWVVGN
                     YYFC"
     gene            complement(6407..6784)
                     /locus_tag="VLI90_15875"
     CDS             complement(6407..6784)
                     /locus_tag="VLI90_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15740.1"
                     /translation="MADQMRPASKEQAPDPATSYERARPEKEAGMGRLDNNMHATPTP
                     RTDSGPDAVKNAQDGSKQINGHEEVNPQKTLPQQPDHTMMEEEPLGWDQAPEDIHDPR
                     RQRQPRTGGKGGTPNVGEPDSAG"
     gene            6918..7829
                     /locus_tag="VLI90_15880"
     CDS             6918..7829
                     /locus_tag="VLI90_15880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSV15741.1"
                     /translation="MKAFPDFRSAAAAVLDYLHRRLGFDLWMVTRVEGQEWIILQVED
                     HGYGIKSGMALKWADSFCIRMIEGQGPCVSPRVLDVPAYAAAPVGQVVKVGAYIGIPI
                     MKEDGSLFGTLCAIHPSSQPDMIVMEQPMIELFTRLLGTILAEELKTLEGARRDERTS
                     AGAHRDPQTGLFGREAWETLVRAEERRCVDYGNPAAVAVVTLRASDRQLHEGLLERAA
                     GALTQATREQDVLARVGDHQFAVLAVECDRAGMKALVGRLTDALAEVRIDASISVSHR
                     CPPRTLWHSWREAEGEAEGNSRDHSHG"
     gene            7980..8894
                     /locus_tag="VLI90_15885"
     CDS             7980..8894
                     /locus_tag="VLI90_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSV15742.1"
                     /translation="MSWRRYTDWAKQRQATLRLADFLGRGRGRMRFLDHLNLPPAARV
                     KPDLSNWENQELAAVWIGHATVLLRIGGRTILTDPVLSTRVGIGLGLMTGGPRRLIAP
                     AISLRELPPIDLILISHAHFDHLDRPTLARLPKRIPVVAAHHTGDLISDLGFRHVKEL
                     RWGESARAGGLMITAREVRHWGARTFYDRHRGFNAYLIESSRHHVLYGGDTAYHEGFT
                     DLPKVDLAILGIGAYDPYVAAHATPEQAWRMGDHCRADRILPIHHSTFRLSHEPTGEP
                     IQRMLTAAGRDADRIVAREVGHQWALEN"
     gene            complement(8934..9962)
                     /gene="trpD"
                     /locus_tag="VLI90_15890"
     CDS             complement(8934..9962)
                     /gene="trpD"
                     /locus_tag="VLI90_15890"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSV15743.1"
                     /translation="MTQAPLRDILLKLCRREDLTRDEARSAFDHIMSGEATDAQIGGL
                     LVGLAAKGTTVEELVGAATVMREKVLPIGCDREEIIIDTCGTGGDVRGTFNISTAAAI
                     IAAAAGVKVVKHGNRSASSKSGSADVLERLGLKLEVSHAVLDRCLAEANICFAFARYH
                     HPAMKFVGAARTSLGIPTIFNLLGPLTNPAKARHQLLGVFAPELTDRLAAVLRELGSE
                     RAWVVHADDGLDELSTIGPTRVSELRDGHVKTWRLDPKQLGLPYARLSDLQVNSVDEA
                     ADRLQRVLAGEAGPMRDVALLNAAAALVVAGRSDEIGEALKLVAEAVDSGRAKQTLDT
                     LIRIASAQ"
     gene            10173..11171
                     /locus_tag="VLI90_15895"
     CDS             10173..11171
                     /locus_tag="VLI90_15895"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HSV15744.1"
                     /translation="MPHSLQDKFRGCLLGGMLGDIAGAVVEAESPGYIAKTYRSVDDI
                     LCTDSVPEFSGPDWRVGRFTDDTQMTLCVAEWLIAGEAPAAELLLARFAEAWQPWRRY
                     GPGTETILRMFPQHRAEWRSLSTAMFPEGSHGNGSAMRVAPIGLARADDLDSVADLAI
                     ESSRPTHSHALAYQGAVLQATAVATAVSLTRFDLAMFLGALRKSLRRFDDLLQSTTAL
                     SDAIDAIENGLARGASCLEISSVLGTGVSATEAVPMAIYCFVRHPDSFTHVIHDAVFI
                     GGDTDTIASMAGAISGAFLGSHVIPTSWLGAVREERYTVAAVQDVADRLFAKCARP"
     gene            complement(11186..>11371)
                     /locus_tag="VLI90_15900"
     CDS             complement(11186..>11371)
                     /locus_tag="VLI90_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15745.1"
                     /translation="PSVGLSMISGGRLTDHREFTAVSAILIRNVSLFHVLRIGPRTHR
                     FWRPGDPASNFFRSDVP"
BASE COUNT         1978 a         3492 c         3787 g         2114 t
ORIGIN      
        1 atgcgtcacc ttcgcgcgta gctcttggtt cgatcgaaag ctaacggcgt cgtaatcatc
       61 cacccatacc gaaccggcgg agcggatgta gccggcggcc tggcttgaat gcaggacgtc
      121 gatgaacggg tgatcgccgg cgaacggatc gagctgcagc agctcggcca cgcgcgccag
      181 tcgtcgtgcg atctccggcg ttgcgcgctg gcgcagttgg tcgtcgctgg tgatgcgctg
      241 gctctggcac cagcgccaga ttgcctcaaa ctcgcgctcc agcacgtcgc cggggcaatg
      301 gcggaacagg aagaacccgc tcgtgcacag gccttcgagc acgcgctggt gcaccgcgcc
      361 gtgcgggctg atctgcaagt tgatccggct cgcttggtag atttttgaaa gttgatggac
      421 gttgtcagcc acgccacggg cgaatcgccg cagcgtcggg tgtgattccc aaccgcggcc
      481 gtagaggtgc acgttcacgc cgagtccggc gagccactcg agcgactgat ggcggaacag
      541 cgcgttgttg atgcgctgcg tgaacaggtc catcagcgcc ggcatttgct cgggcggcag
      601 cgtcgtttgc gtgtcgatca tcgcggcatc gacgatgcgg cggatggtga tcggctcagt
      661 cacactgccg ccggcgtcgt agatcgattt gatctgttcg aagatcaccg tcagcaaccg
      721 cgttgcctgc ggcgtgtcga gccgtgtgat ttcagcttgt aaaatcgatt ccgcgggggt
      781 gctggcgttg ctgacgaacg agacatcgca ggtgaaggcg tcgcgctcgg agggcgtcag
      841 cgcttggggg gcgaagcgct gttcgttgac ggcgacgatc gcgggcatgt atcgggcggc
      901 ggggtagccg aactcgcgcg tcatcttcag gtgcgcaaag ccgagtgcgt aatcgcgctc
      961 gccctgggcc gccccggcct tgggggtgaa caggttcgag agggcatcct gcacccacat
     1021 cacgaccggc aggttgtcgg ggaagccgcc catttcggtg cgatagtggt cgatgatgac
     1081 cgccaggtcg ggatggaaat cggcgcaggc ctgggcgacc gagaaattgt tgggcacttc
     1141 atgatcggcc ggctcgatga acaggcgcgt ctcgtgcccg aggcgatcga acgccgccag
     1201 ccagtcgcgc atcgagtgct gcaggaaggt ggtgtagcgc gaggtgacac cgaggattcg
     1261 cagcggccgg ccggattgaa acagtgatgc gatggaggcc ggtgtcgcac cggcgtacat
     1321 caaccgcagg cgctggttca gctgcgtcag gcgctcggtc atcgccgcac gggcggcggc
     1381 gagcacggtg tcgatcgtct gcccggccgg ccagagcgcc gggtcgatcg tcaccgacag
     1441 cttgggccag gggcattgcg aatcgtgcac gaggcttcgg cgaaattgat cgaacgcatc
     1501 atcgccggcg aagagccgca cgcgcgcatc ggccagcagc tgccgccagt cctgcaggtg
     1561 caggatgacc cagagccgct cgacgtcgcg gatcaggcaa tgcatgggcg ggcgataacc
     1621 gggcgcggtg gggacgttgc acggcagcgc gtagagccgg ttccagagcc agcccaaatc
     1681 ttcaccgctg accaggaccg gttggtcgta attatcatca ctgaatagcc gcttggccag
     1741 atcgagcgcc gccaccggca gcagcggatg cggcagggca ttcaccgcgc cgatcgagcc
     1801 agcgcccaga cgcacgtgat cggggtacgc ctggatgaag tacgtctggc tcggcgataa
     1861 ttctcgcagg cgctgggcca gctgcggata tcgcgcggcg agcgcatcga cgttggcgat
     1921 gaagcgatgc ttcagcagtt ccagcggcgt ccacgaagct tgcggtgcgg cgacagccaa
     1981 gaatgccttc tccctcacga cgcgagccgg gccggctcgt aatgcgcctg cagcgcgctg
     2041 gggttgatgc ggtcgagcaa ttgctcggcc gtcaccggtt gattgccgat ctgttgcact
     2101 tcgatggccg cggcaagcga accgagatac gcggcggctt gcggggatcc accggcggcc
     2161 agtgccaggc tcgcgatcgc cagcagcgca tcaccacagc ccagcgggtc taccgggtgc
     2221 gacgccatcg ccggcaggta cgccgtgcgc agcttgcggt cccacgaatc gctcagggcg
     2281 ttcgtctgct gttgctggtc gaacaggcac agcccctgtt tgcccagcgt gatcatcgct
     2341 gtcttggcgg ccgtcgtcgc gagcagctcg tacacgacag cgttcagtcc gctggaaaaa
     2401 ttgttcagcg tctggcggac ttcgcgctcg gtggggcaga gcagatcgac gtccttgaac
     2461 cgcagcaacg tgctttgtcg ccccgacacg tctgcggtga gcagcggcac ccgcgggcgg
     2521 atgatcggca tcacgcggtc cagcagcggt ccggtgatca ggccatatcc gaagtcggcg
     2581 aagatgacgc ccgcggcgtc ggtggcggct tcttcgatca gccacgcggc ttcatccacg
     2641 ctgcgcgaat ccagcggcgt tgtcgcgcct tcatcaactt tcaacagctt ggtctgatcg
     2701 accaggtatc ggctcttgct gaccagctgc ttgcgctggc gaagcgcgcg caccgtgacg
     2761 ccggcttcgg tgagcctggc gacgacctgt cgcgatgcat cgtcatcggc gagcgacgtg
     2821 atcagcgtcg catccgcgcc gaggccggca aggtgccggg cgatgatggc ggcggcgccg
     2881 tcgtactgct ccgactgcag cgggcgcagc gtcatcatcg ggccttcgct ggccacacca
     2941 gtcgcgtcgc agaagtggta gcgatcgcag atgtaatcac cgacgacgac catgcgtttc
     3001 ccacggcagc gcaggaccag gtttgatagc gcggcagcgc tgagatcgaa ctgctggcgg
     3061 aagcggccga ccttctggtc gttgaataaa gttgcggttg cgtccatcgc gccgatcaac
     3121 gcggtggagg agtagatgac gtcgccgctg gagaacacga cgcgcccgcc gttgcgggtg
     3181 acggtgtcgc gctcggccag aaagcggggg tcttcgttgc gttcgtactc gcggcccttg
     3241 acgtagatgt cgggcttgag cgcgtcgagg acctcgactg ccgtcggatg cggattgatg
     3301 tagacacaat cgacgcattc gagggcggcc aggctggcgg cgcgaagatc gtctggaatc
     3361 agcgggcgat tgatgccttt gttcacctgc ggatcggcgc tgacggtgac gatcaaggca
     3421 tcgccgagcg agcgggcgaa ttgcaagtag tggatgtggc cggggtgaac aatgtcgaag
     3481 cagccgtggc agtggacgat ggtcttgccc gcggcggtgg cgctgcggcg gaactccagc
     3541 agctgcggga gggagcagat tttgcgatgg gcggtgttgg acatgtgtgc gcacctgcgg
     3601 acgtccttgt ccgtggggtg cttatcggcg tgggaggggg cggggcgtga atttatcgga
     3661 cggtgacgcg tgatcgaacg ccttaaccgc tgaagcggtt agtccggccg gaggttctgc
     3721 gcgcattcga tggtttctga aattacgcgg cgcgttcgcg gtataggcgc aggaggttca
     3781 ggcgacgctc gtccaggtcg agcatcgcct gcatgaatct tcccggcagc gttgcggtta
     3841 gggcggcgtc tcgctcgctt ggcgttgttc gcgcgatcga ctgccgactg accacgaacg
     3901 acatatcgta gaacccgaag atggctggga cgaactcgat gtgctcggca tccaactgtt
     3961 ggaacagctt tcgcgcggcc gttgggctga agagaaacag gtgttcgttc gggtcgagca
     4021 tcatcgagaa cttgtgaccg atcgcgcgca gttccggcag cgttgcgcct tcgggataac
     4081 tcggtgtctg gatcagcagc acgccatccg actttagcag gtcgaggcat cgccgcagcg
     4141 ttgcctgcgg ttgcggcagg tgctccatga cgtccatcag gatgaccgcg tcgagcgtgc
     4201 cggcggcgat ggtttggtct tcgattggac cgctcaagac cggaacatcg aaggtctggc
     4261 gggcgtattc gacgatggat gggctgagtt caagtcccgt cgcatcgaag ccgctctggt
     4321 gcagcatcgc gacgaacccg ccgtgggcgc agccgatttc cagcactcgc gccggcggca
     4381 gcttgaactg aagcagcgtc cgaagccagt gaatgcagcg gtcggacaag tcggtgcgcg
     4441 accgcgtgat gatgtcgggg caattcagct cgcgcgtttg gtgatcgaac caataatcgc
     4501 ggccgtatag atcggcgcgg tcgtcgatca cgcgcgggtc ggcctgccga tcggtgatgg
     4561 cggcgacgag cgtctggcac gcgtcgcagc ggtgatagcc ttcgccccat tcgatcaacc
     4621 gctcgttgcc gcaccagcat cgcatgcagc tgttatcggt cccggcgaac gtccgaataa
     4681 acttttcacc ctggtcgctg ggcacgtccg atattgctga aatcaacggc aaccgcagat
     4741 cgaggtcctg acgatgattg actcgactat gaccctcacc gagcaaacga agcgcaagac
     4801 ggcgcggcaa tacgaccatt cagctttcta tcccaccgtg cggccgacgg acgtgtttct
     4861 ctgcagctat cccaagtccg gcacgacctg gctcggcttc ctcatcgcgc aggtgttgaa
     4921 gaaggacgag tcggaagaac tcgatctgaa gtcgttcaac aagtacgtgc ccgacgtgaa
     4981 tctgcaatac acgaagcggg gctcgctggc gcagcacgcg gcgctgccgg acccgcggtt
     5041 tttgctgtgt cacgcgacgt acgatgcgaa gctgccgaag gtcgtctacg tcacgcgcga
     5101 cccgcgcgac gtgatggttt cgtactggca ttaccagaag tttttgaagg aagacttcaa
     5161 cctgtcgctg gcggactttt tgcgaagcga cgatcactgg ccatgcgagt gggacgagca
     5221 cgtggccgac tggctgctgc ccgagccgca cggcaacgtg ctgcgggtgg gctacgaaga
     5281 gatacaccag gatgcggcgg gcgtgctgcg gcgggtgctg caattcggcg gcgtcaaagc
     5341 gagcgaagcc cgaatcaccg cggcggtgga atgctcgcgc ttcgagaaca tgcgcgccgc
     5401 cgaggaacga tttggcgtaa acggaaaagc cggcgacgaa caggagcgct tcgtccgcaa
     5461 gggacgggtt ggttcgtggc aggacgagat gggctacacc gagctgcgca tcgtcgaaga
     5521 gcgctacggc cgggtgatgc gccaagtcgg atacgaaccg ctcagttgag tagaaccgac
     5581 caaggcccgg ccgcttcggt ggccgggaca taccccgcat gctctggtcc accgatccca
     5641 agctcgagtt catcaactac tatggcaact ccagcctgtt tgcgctggac atcctgcgcc
     5701 gccacggacc gctcgatcag atgaagctgt tcagcatcgg ctgcttgatg ccgcacctgg
     5761 cggattactt ggcgctgttt cacacggtcc accacaattc gctggtgacg acgaaactgc
     5821 cgatgccgcc cgaactgttt ccggcgaagt tcgtcgaaca ccagaccgac ttctttgatc
     5881 tgccgccgct cgacatcgac tgcgcgatct cccatgtggc gatccactgc ttcaacgaca
     5941 cgcgctatgg aaacgccagt tcaaatgatg gttggcaaaa gccgtacaag gccgcggcga
     6001 aattgcggca gatggcgggc ccgcgaaagc tgccggccat cgtcagcgtc gcggtgaacg
     6061 agcaggaagg tttttacgac aacaacgtgc acctgtcgca cgcgcggttc atcgcctcgt
     6121 tcgaagccgc gggcttcaag cttcgcgaat acttcttcga ttacgtctgc ggcgggacga
     6181 catacaaacc ggagtatctg gagtacgaat accggcggtc gaagaaatta cccgggcacg
     6241 cggaatcgcc gcggcagtgg gtggtgggga attattactt ttgctgaatt ggctctgcgc
     6301 gcatgtgagc cgcttgcggc ttcgcagatt tgtcacgatc gatcggcgaa tcgaacgtga
     6361 tgggtctgcg aagccgcaag cggctgtggt ttgcgcgctc gcccgttcat ccggcgctgt
     6421 cgggttcacc cacgttcggc gtgccgccct tgccaccggt gcgcggttgg cgttgtcggc
     6481 gcggatcatg aatatcctcc ggcgcctggt cccaccccag cggctcctct tccatcatcg
     6541 tatgatccgg ctgctgcggc agcgtcttct gcgggttcac ttcttcgtgc ccgttgatct
     6601 gcttcgatcc atcctgcgcg ttcttaaccg catccggtcc actgtcggtt cgcggtgtcg
     6661 gcgtggcgtg catgttgttg tcgagccggc ccatgccggc ttccttttcg gggcgggctc
     6721 gttcataact ggttgccggg tcgggcgcct gctccttcga cgccggccgc atttggtctg
     6781 ccatgatgct gctcctttcg cgcggaaaac cttgctcgat ccaatctatc gcaatgcgca
     6841 ccgcagactg aacggacaaa atggtttcac gcgcacgcgc gcgagtcggg tgatataatg
     6901 ttcgcttcat gctttccgtg aaggcattcc ccgatttccg cagtgctgcc gccgctgtgc
     6961 tggactacct gcaccgccgg ctggggttcg atctgtggat ggtcacccgc gtcgaagggc
     7021 aggagtggat catcctgcag gtcgaagatc acggctacgg catcaagtcg ggcatggccc
     7081 tgaaatgggc cgactcgttc tgcatccgca tgatcgaagg ccagggcccg tgcgtcagcc
     7141 cgcgcgtgtt ggatgtaccg gcctacgccg cggcgccggt ggggcaggtc gtcaaggtcg
     7201 gggcgtatat cggcatcccg atcatgaagg aagacggctc gctgttcggc acgctgtgcg
     7261 cgattcaccc ctcatcgcag ccggacatga tcgtgatgga acagccgatg atcgagctgt
     7321 tcacccgact gctgggcacg atcctggcgg aggaattgaa gacgctggag ggcgctcgac
     7381 gcgatgaacg gacatcggcc ggcgcgcacc gtgatccaca aactggcctg ttcggccgcg
     7441 aggcgtggga gacactcgtt cgcgcggaag aacgtcgctg cgtcgattac ggcaaccccg
     7501 ccgccgttgc ggtggtgacg ctgagggcat cagatcggca actgcatgaa ggtctgttgg
     7561 aacgagcggc cggcgcgctg acgcaggcga cgcgcgagca ggatgttctg gcccgcgtgg
     7621 gggatcatca gtttgcggtg ctggccgtcg agtgcgatcg cgcgggcatg aaagcgctgg
     7681 tcggccgact gaccgatgcg ctggcggaag tgcggatcga cgcgtcgatc agcgtatcgc
     7741 atcggtgtcc gccgcggacg ctgtggcaca gttggcggga ggcggaaggc gaagcggaag
     7801 gcaactcgcg cgatcattcg cacgggtagg ggaatggaaa atgtgaaacg gtaaacggca
     7861 aatggacgga ttgcgcttcg ccctttcttc cattttccat ttaccttttt ccactttcca
     7921 ttcccgaacg gcacctcgcg acgggcgtat gactcttttc agccggagcg tgagaaccct
     7981 tgagctggcg acgatatacc gattgggcga agcagcgtca ggcgacgctg cggctggcgg
     8041 atttcctcgg ccgcggccgc gggcgcatgc gcttcctcga tcacctgaac ctgccgccgg
     8101 cggcgcgcgt gaagccggat ctgtccaact gggaaaacca ggaactggcc gcggtgtgga
     8161 tcggtcacgc caccgtgctg ctgcgcatcg gcgggcgaac gatcctgacc gaccccgtgc
     8221 tgtccacccg cgtcggcatc gggctgggct tgatgaccgg tggcccgcgc cggctcatcg
     8281 cgccggcgat ttcgctgcgc gagttgccgc cgatcgatct gatcctgatc tcgcacgcgc
     8341 acttcgatca cctggatcgg ccgacgcttg cgcgcctgcc caagcgcatt cccgtcgtgg
     8401 ccgcccatca cacgggcgat ctgatcagcg acctgggctt ccggcacgtg aaggaactgc
     8461 ggtggggcga gtccgcccgc gccggcgggt tgatgatcac cgcgcgggaa gttcgacact
     8521 ggggcgcgcg cacgttctac gaccgccatc gcggcttcaa cgcgtacctc attgaatcat
     8581 ccaggcacca cgtgctgtac ggcggtgaca ccgcgtacca cgaggggttc acggacctgc
     8641 cgaaggtgga tctggcgatc ctgggcatcg gggcgtatga cccatatgtc gccgcccacg
     8701 cgacaccgga gcaggcttgg cggatgggcg accattgccg ggccgatcgc attttgccga
     8761 tccatcacag cacgtttcgg ctgagtcacg agccgacggg agagcccatc caacgcatgc
     8821 tgaccgcggc ggggcgcgat gcggatcgca tcgtggcgcg cgaggtgggc catcagtggg
     8881 cgttggaaaa ctaatcgacg gcgacgcgtc tggtttgcga cgccgcaagc ggttcattgt
     8941 gcgctggcga tgcggatgag cgtgtctaac gtctgcttcg cccgccccga atccaccgcc
     9001 tctgcaacga gcttgagcgc ttcgccgatc tcgtcagatc tccccgcgac taccagcgca
     9061 gccgcggcgt ttagcagggc gacatcgcgc atcgggcccg cttcgcccgc gaggacgcgc
     9121 tgcaggcggt cggccgcctc atcgacgctg ttgacctgca ggtctgacag ccgggcgtac
     9181 ggcaggccca gttgcttcgg gtccaaccgc cacgtcttca cgtgaccgtc tctcaactcg
     9241 ctgacgcgcg tcgggccgat cgtggatagc tcatcgagtc cgtcatccgc atggacgacc
     9301 cacgcgcgct cgctgcccag ttcgcgcagc acagcggcca ggcgatcggt cagctccggt
     9361 gcgaagacgc cgagcagttg atgccgcgcc ttcgccggat tcgtcagcgg cccaagaagg
     9421 ttgaagatcg tgggaatgcc gagggatgtg cgcgccgccc cgacgaactt catcgccggg
     9481 tggtggtatc gtgcaaaggc aaagcagatg ttcgcctcgg ccaggcagcg gtccagcacc
     9541 gcgtgcgata cttccagctt caggcccaat cgctcgagca cgtcggccga gccggacttg
     9601 ctcgatgcgc tgcggttgcc gtgcttgacg accttcacgc ccgccgccgc cgcgatgatg
     9661 gccgccgccg tggagatgtt gaacgtcccc cgcacgtccc cgccggtgcc gcaggtgtcg
     9721 atgatgatct cttcgcgatc gcatccgatc ggcaggacct tctcgcgcat cacggtggcc
     9781 gccccgacca attcttcgac ggtcgttccc ttcgcggcca agccgaccag caaccccccg
     9841 atctgcgcat cggtcgcctc gccggacatg atgtggtcga acgcggagcg cgcctcatcg
     9901 cgcgtgaggt cctcccggcg gcaaagcttg agcaggatgt cgcgcagcgg ggcttgggtc
     9961 atcgcgcgca aaactacggc aatctgacgc cacggtaaag gccggggtgg aatgacggcc
    10021 gtccgacgtc ccggcgcact tgcgcaagtg agacattcag gtcgatgatg ggacattttt
    10081 aggaaaatgt cccaaaatgt ctcatcggcg cgcggtggaa aaacgcgatg aaagttgcag
    10141 ggatctccat ggcaccatca caatgcccat cgatgccaca ttcgctgcag gacaagttcc
    10201 gcggctgcct gcttggcggg atgttgggcg acatcgccgg ggcggtggtc gaggcggaat
    10261 ctcccggcta catcgcaaag acttaccggt ccgtcgatga cattctttgc accgattcgg
    10321 tccccgagtt ttccggcccc gattggcgcg tcggccgctt caccgatgac acgcagatga
    10381 cactgtgcgt ggccgagtgg ctcatcgcag gcgaagcgcc ggcggcagag ctactcctgg
    10441 cgcgcttcgc agaggcctgg caaccgtggc gtcgttacgg gcccggcacc gaaacgatcc
    10501 tgcggatgtt cccgcagcac cgcgcggaat ggcgcagcct gtcgaccgcg atgtttccgg
    10561 aaggttccca cggcaacggc agcgccatgc gcgtcgcccc gatcggcctt gcgcgtgcgg
    10621 acgatctcga ttccgtcgcg gacctcgcga tcgaatcatc tcgcccgacg catagccacg
    10681 cgctggcgta tcaaggcgcg gtccttcagg cgacggcggt cgccactgcg gtgtcgctca
    10741 cgcgctttga cttggcgatg ttcctgggcg cgctgcggaa atccctgcgg cgcttcgatg
    10801 atcttctcca aagcaccacg gcactttccg acgcgatcga tgcaatcgag aacggcttgg
    10861 cccgcggcgc gagctgtctg gaaatatctt cagtcctagg caccggcgtg agcgcgaccg
    10921 aggccgtgcc gatggccatc tactgctttg ttcgccaccc ggactcgttc acccacgtga
    10981 tccacgacgc ggtgttcatc ggcggcgaca ccgacacgat cgcgtcgatg gccggcgcaa
    11041 tctccggcgc attcctcggc agccacgtca ttcctacaag ctggctcggc gccgttcgcg
    11101 aagagcggta cacagtcgcg gcggttcaag atgtcgccga tcgcttgttt gccaaatgcg
    11161 caaggccgtg attctcggtc gcgcctcacg gcacatcgct gcggaagaag ttgctcgctg
    11221 gatcgcccgg ccgccagaag cggtgcgttc tggggccgat gcgaaggacg tggaacagcg
    11281 acacgttgcg gatgaggatc gcgctgacgg cggtgaactc gcggtggtcc gtcagcctgc
    11341 cgccgctgat catagacaag cccacggatg g
//
LOCUS       DATIMH010000914         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3968350, whole genome shotgun sequence.
ACCESSION   DATIMH010000914 DATIMH010000000
VERSION     DATIMH010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3968350"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /gene="purH"
                     /locus_tag="VLI90_15905"
     CDS             complement(<1..1298)
                     /gene="purH"
                     /locus_tag="VLI90_15905"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HSV15746.1"
                     /translation="MPDLVPVKRALLSVSDKTGLAEFATALHKEFNVELISTGGTAKF
                     LRDLGLPVTDVSAVTGFPEMMDGRVKTLHPKIHGGLLALRDDPEHAKAMREHDIQPID
                     LVCINLYPFAKTIARPNVRFEDAIENIDIGGPSMIRSAAKNHAYVLVVTSPERYEKVL
                     GDLRQHAGSSCGKHRLKQAQRAFAHTAEYDALIAQYLAKVLTPEVTDTPAPQDVALAA
                     ELDLHLHKQQDLRYGENPHQKAALYVDRQPKEASVAYAKQLHGKELSYINLLDADGAL
                     SAVKEFSTPAACIVKHTTPCGCATAADLPTAFRRAFDGDPLAAFGGIVALNKPIDLAT
                     AQAITGIDKLLEVIVAPGYADDALRLLRERWKNVRLLDVGPVDQFDPAELHLHKIVGG
                     YLVQQRDLTGVDDAGWKVVSNRKPSDAEMSDLKFAWLVCK"
     gene            complement(1832..>3096)
                     /locus_tag="VLI90_15910"
     CDS             complement(1832..>3096)
                     /locus_tag="VLI90_15910"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSV15747.1"
                     /translation="LRGRAYACATMLASALVAATLQFGGTGCTEPVTYKANAVAELPA
                     DSFVKAWMADLHLKDASIQRIDVREKMLYVVSSNKQVTAINRGPGTIKFVAQVAQAGE
                     RLLPPVELTDKIVFPTATSLEIYDYTGVHQRSVNLDAPIRSGATGGGSMIYFGADDPA
                     GGRVEAFDLTRNFANRRWELLTPGGSISSTPVLYLGVLFVGTESGAVYAVNEERKPIW
                     ATEGSVFQASGPIVADLKADENGLYVASKGSILYCINRTNGKLQWQYFSGGPLYTAPV
                     PTSDTLYIYSEGVGIAAVSKAPVPTSNGNAQNAYDRSAKWVYHPGKQFLAQDEKYAYI
                     METRRDAKDEDVTHRVIVAVDKETGQKMFESQHQDFSVFGTNPRDNTIYAAYPSGELF
                     AIKPVLKPGQIGELVAVPVSAKDAVAMR"
BASE COUNT          541 a          969 c         1008 g          578 t
ORIGIN      
        1 tgtttgcaaa ccagccaggc gaatttcaga tcggacattt ccgcgtcgct gggtttgcgg
       61 ttggagacga ccttccagcc ggcgtcgtcg acgccggtca ggtcgcgctg ctgcacgaga
      121 tagccgccga cgatcttgtg caggtgcagc tcggcagggt cgaactgatc aaccggcccg
      181 acatcgagca ggcgcacgtt cttccaccgc tcgcgcagca gccgcagcgc atcgtccgcg
      241 taacctggag cgacgatcac ctcgagcagt ttgtcgatgc cggtgatggc ctgcgcggtc
      301 gcgagatcga tcggcttgtt cagcgcgacg atcccaccga aggcggcgag cggatcgccg
      361 tcgaacgccc ggcggaacgc cgtcggcaga tcggccgccg tcgcgcagcc gcacggtgtt
      421 gtgtgtttca cgatgcacgc cgccggcgtc gagaattcct tcaccgccga cagcgcgcca
      481 tccgcgtcga gcaggttgat gtacgacagc tccttgccat gcagctgttt ggcatacgcg
      541 accgacgcct ccttcggctg gcgatcgacg tacaacgccg ccttctggtg cgggttttcg
      601 ccataacgca ggtcctgctg cttgtgcaaa tgcaagtcca gctcggccgc cagcgcaacg
      661 tcctgcggcg ccggcgtgtc ggttacctcc ggcgtcagca ccttcgccag atactgcgcg
      721 atcagcgcgt catattccgc cgtgtgcgcg aacgcgcgct gggcctgctt caggcgatgc
      781 tttccacacg agctgccggc gtgttggcga agatcgccca ggactttttc atagcgctcc
      841 ggcgacgtga cgaccagcac gtacgcgtgg ttcttggccg ccgagcggat catgctcggc
      901 ccgccgatgt cgatgttttc gatcgcatcc tcaaaccgca cgttcggccg ggcgatggtc
      961 ttggcgaagg ggtacaggtt gatgcagacc aggtcgatcg gctgaatgtc gtgctcgcgc
     1021 atcgccttgg cgtgctcggg gtcatcgcgc agcgccagca gcccgccatg gatcttggga
     1081 tgcagcgtct tgacgcggcc gtccatcatc tcggggaacc cggtgacggc cgagacatcg
     1141 gtgaccggca ggccaaggtc gcgcaggaac ttggcggtgc cgccggtgga gatcagttcg
     1201 acgttgaatt ctttatgaag cgcagttgcg aactcggcga ggccggtttt gtcggagacg
     1261 ctgagcaacg cgcgtttaac ggggacgagg tcgggcatag tcgcgcgaaa ttaaggcgcg
     1321 gcggagaaaa agggaaggac gcggggagtg gtcgagtagg cgaggaacga agacgagggt
     1381 agcgaggatt aagtcaggca gtggcacggg cgtctcgccc gtgccgaggt gctcagacgc
     1441 gggcgagacg cccgtgctac gaatacagct atctgacgct ctatttactt tgaatcaccc
     1501 tgccacactc cgggcagacg ccgctctgat ttcccgtgag aggatatccg caatggatac
     1561 atcggtgatg gcgagctcga tgaaactcca cgagcgattg gcgcacaccg atcattgaca
     1621 gaatcagcag cagcacccca aagacgatat ggcctgccac aggacgcgca aaggacggac
     1681 cgtccacgag cggccgtcgc agcacgatct cgaacgccgc gccagttagg aggaccgcgg
     1741 caccaagaag cgcgcagccc gaaccgatga cgcttgacga ttcctcaccc gcatgatcgt
     1801 tcaaccggcg gtcgcccgac agaaatgggt gtcagcgcat cgccaccgcg tccttcgcgg
     1861 acaccggcac cgcgaccagc tcgccgatct gcccgggctt cagcaccggt ttgatcgcga
     1921 acaactcgcc gctggggtac gcggcgtaga tcgtgttgtc gcgcgggttg gtgccgaaga
     1981 cgctgaagtc ctggtgctgc gattcgaaca tcttctggcc ggtctccttg tccaccgcga
     2041 cgatcacgcg gtgcgtgacg tcttcgtcct tcgcgtcacg ccgggtttcc atgatgtagg
     2101 cgtacttttc gtcctgcgcc aggaactgct tgccggggtg atagacccac ttggcggatc
     2161 gatcgtaagc attttgcgcg ttgccgttgc tggtcggcac cggcgctttg ctgacggcgg
     2221 cgatgccgac gccttcgctg tagatgtaga gcgtatcgct cgtgggcacc ggcgcggtgt
     2281 acagcggacc accggagaag tactgccatt gcagcttgcc gttggtgcga ttgatgcagt
     2341 aaaggatgct gcccttggag gcgacgtaca agccgttctc gtctgccttc agatcggcaa
     2401 cgatcgggcc ggatgcttgg aagacgctgc cttcggtggc ccagattggt ttgcgttctt
     2461 cgttgacggc gtacaccgcg ccgctttcgg tgccgacgaa caacacgccc aggtaaagca
     2521 ccggcgtgct gctgatcgac ccgccgggcg tgagcagctc ccagcggcgg ttggcgaagt
     2581 tgcgggtgag gtcgaacgcc tcgacgcgcc caccggccgg gtcgtcggcg ccgaaataga
     2641 tcatgcttcc cccgcccgtc gcgccgctgc ggatcggcgc gtccaggttc accgagcgct
     2701 gatgcacccc ggtgtaatcg tagatctcca gcgacgtggc cgtcgggaaa acgatcttgt
     2761 cggtcagctc gaccggcggc agcaggcgct caccggcctg ggcaacctgg gcgacgaact
     2821 tgatcgtgcc cggcccgcgg ttgatggccg tcacctgctt gttgctcgac acgacgtaca
     2881 gcatcttctc gcgcacgtcg atccgctgga tcgacgcgtc cttcagatgc aggtcagcca
     2941 tccacgcctt gacaaaactg tcggccggca gttcggcgac ggcgttggct ttgtacgtga
     3001 ccggctcggt gcaaccggtg ccgccgaatt gaagcgtcgc cgcaaccagc gcgctggcca
     3061 gcatcgtggc gcatgcgtaa gcgcgaccgc gaagac
//
LOCUS       DATIMH010000915         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2817786, whole genome shotgun sequence.
ACCESSION   DATIMH010000915 DATIMH010000000
VERSION     DATIMH010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2817786"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VLI90_15915"
     CDS             <1..369
                     /locus_tag="VLI90_15915"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSV15748.1"
                     /translation="RMADVVKSPLLAATVALLLGSVALGLVTATGWLGRGNLGQVVRV
                     PWWVWVAGAMSLFTVVSILALPKVGAEVVVAGTMFGQLTAGALLDHFGWLGVPQVPMN
                     AWRIAGAVVLLAGVWLMQHK"
     gene            636..872
                     /locus_tag="VLI90_15920"
     CDS             636..872
                     /locus_tag="VLI90_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15749.1"
                     /translation="MEPGSGIAPPPITATSANAWANNALFGNGGNNTPFGGGGNDQIH
                     AKNENNDYVDGGSGNDSAVIDPIDTVVNVEMVTH"
     gene            1049..2350
                     /locus_tag="VLI90_15925"
     CDS             1049..2350
                     /locus_tag="VLI90_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15750.1"
                     /translation="MPTSRHAALRAACMFSPLLAALPAAGIPDIPNITLSITNQGPAF
                     DNFGDSTTGWTGYLISASADDPTETIRSFAADTEARLKLGILGTLLQDWSPTKGGFIP
                     SAVRSSLSETNGGPTGTDSHLLALPTYFFSGTEDNDLTHPTGAPANIPGSDVWGTGQA
                     LHGVVQVPSNDRHNSQPLAYVVTKDSTAASYHLTVADKDLRTYEFFGRFDTTGVTTPL
                     IEAIAAPSFNGFVGGIGTIIADTHNPLHCDTATFSPRDPSDTTIPLDIRPTGLAFASN
                     INGGAGTTKDYIGVRGFQPDLGELFALNLKINGSDPTGAQVQQLIADINANNIGGAVA
                     QAADAQLFPGYDLMVTTPYIDNPDGTGDWFAFDFSHEANVQGVIVTDIAAAQAVPEAS
                     MIGVLAVVAIGLRRRRASRLGNRSAQTDSIDIVVKVERIAR"
     gene            2371..>3429
                     /locus_tag="VLI90_15930"
     CDS             2371..>3429
                     /locus_tag="VLI90_15930"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HSV15751.1"
                     /translation="MSRNQTLDRAVAVVVEAMERRTLLSGSAILGAGGTLTVTGTGAA
                     DIIGLEVDEDGSVAVTVNGANKQFVANTVKTIVINSGGGADSIAVDISDENINQIPET
                     INAGDGNDTVTIDSVVQIGPTVVVNCGAGNDSVTLNYDGDSTVNGGDGNDTLEASANF
                     NEDVCHMFGDNGNDTLHSVSAGAGGNLLAIMSGGNGNDTFRNDDIDNRINATGGPGID
                     TIQLGEGSSGIIALDGVDHGSNTIAHADVENVVGSPFGDVDIIGNALNNLIDVGSDSA
                     VTVLGGGGNDTITASGDTTYLDGGSGNDVITCDSTQPGTTLIGGDGNDTLIGGDAPDK
                     LFGGNGNDRLVGNAGSDTM"
BASE COUNT          667 a         1128 c         1073 g          561 t
ORIGIN      
        1 cgcatggccg acgtcgtgaa atcgcccctg ctggcggcga cagtcgcgct gctgctgggc
       61 agcgtcgccc tgggcctggt caccgccacc ggctggctcg gccgcgggaa cctcggccag
      121 gtcgtccgcg tgccatggtg ggtgtgggtc gcgggggcga tgagcctctt cactgtcgtg
      181 tcgatcctgg cattgcccaa ggtgggcgct gaagtggtcg tggccggcac gatgttcggc
      241 cagctgaccg ccggcgcgct gctcgaccat ttcggctggc tcggcgtgcc gcaggtcccg
      301 atgaacgcct ggcgcatcgc cggcgccgtc gtcctgctgg ccggcgtctg gttgatgcag
      361 cacaaatgaa cccgccagcc ggaacgttca atactctgga tccccctttt ttggcggcgg
      421 cccgcagggc cgagcgttca gcgccaacgt ccgcagcgag atccatccca gacgagcttg
      481 cgaaagtcgt agtcgaaaca cgaacgccgc aacgacggcg gccccctact cagagacgag
      541 ccgccgcggc ggagggtttt gttggtgctc gcgcggttca aatcggacga cgtcggcggc
      601 gcagcaggga tagcgatccg acaccgagca aacggatgga accgggttcc gggatcgcgc
      661 cgccgccaat caccgcaact tccgccaacg cttgggccaa caacgccctc ttcggcaacg
      721 gcgggaacaa cacccccttc ggcggtggcg ggaatgacca gatccacgcg aagaacgaga
      781 acaacgatta cgtggacggc ggcagcggaa acgactcggc ggttatcgac ccaatcgaca
      841 ccgttgtgaa cgtcgagatg gtcacgcatt gacacgtagc atgggcgtct cgcccgtgcc
      901 gggggttccc cctatgtcgt ttcgcgctca ttccctgatg gcgcgtcagc tacgctgcca
      961 atgagaatga tgatgcttgc tggcgtccgc cgcgcgaagt agcctttcgc gtgcatcatt
     1021 tcggcgagca gaccaacagg agcagcacat gcccacatcg agacacgcgg ccctgcgcgc
     1081 agcctgcatg ttttcaccgc tgctggcggc gctgccggcg gcgggcatcc cggacatccc
     1141 caacatcacg ctgagcatca ccaaccaagg tccggcgttc gacaattttg gcgacagcac
     1201 caccgggtgg accggttacc tgatctccgc cagcgcggac gatcccacgg aaaccattcg
     1261 ctcgttcgcg gccgacaccg aggctcggtt gaagctcggc attctcggca cgctgcttca
     1321 ggattggtcg ccgaccaagg gcggcttcat tccctcggcg gttcgctcct cgctgagcga
     1381 gaccaacggc ggccccaccg gcaccgatag ccatctgctg gcgctgccga cctacttctt
     1441 cagcggcacg gaagacaacg acctcacgca tcccaccggc gcgccggcca acatccccgg
     1501 cagtgatgtc tggggaaccg gacaagcgtt gcacggcgtc gtccaggtgc cgagcaacga
     1561 ccggcacaat tctcagccgc tggcttacgt tgtgaccaaa gacagcaccg ccgcttcgta
     1621 tcatctgacc gttgcggata aggatcttcg cacctacgag ttcttcggtc gcttcgacac
     1681 caccggcgtc acgacgccgc tgatcgaggc gattgccgcg cccagcttca acgggttcgt
     1741 cggcggcatc ggcacgatca tcgcggacac gcacaacccg ctgcattgcg acaccgccac
     1801 cttcagtccg cgagatccgt ccgacaccac gattccgctg gacatacgcc ccaccggtct
     1861 ggccttcgcg agcaacatca acggcggcgc cgggacgacg aaggattaca tcggcgtccg
     1921 cgggttccag cccgaccttg gcgaactgtt cgctttgaac ttgaagatca acggcagcga
     1981 tcccaccggc gcccaggtgc agcagctgat cgccgacatc aacgccaaca acatcggcgg
     2041 cgctgtggcg caggcggccg acgcgcagct attccccggc tacgacctga tggtgaccac
     2101 gccgtacatc gacaaccccg acggcaccgg tgactggttc gcgtttgact tctcgcacga
     2161 ggccaacgtg caaggcgtga tcgtcaccga catcgccgcc gcccaggcgg tgccggaggc
     2221 gtcgatgatt ggcgtgctgg cggtggttgc gattgggtta cgacgacggc gagcaagccg
     2281 tttaggtaat cggtcggcgc aaacggactc gatcgatata gtggtgaagg tcgagaggat
     2341 agcgcgctga agcacttctc gggaggcagc atgtcgcgaa atcagacgct tgaccgagcc
     2401 gttgctgtag tcgtcgaagc gatggagcgg cggacgctgc tcagcgggtc ggccatcctc
     2461 ggcgccggcg ggacgctcac cgttaccggc accggcgccg ctgacatcat cgggcttgag
     2521 gtcgacgagg atggttcggt cgccgtcacc gtgaacggcg ccaacaagca gttcgtcgcc
     2581 aacactgtga agaccatcgt catcaatagc ggcggcggcg ccgactcgat cgccgttgac
     2641 atcagtgatg aaaacatcaa tcagattccg gagaccatca acgccggcga cggcaatgac
     2701 acggtcacca ttgacagtgt cgttcagatt gggccgacag tagtggtcaa ctgtggtgcc
     2761 ggcaacgaca gcgtgacctt gaactacgac ggcgattcga cggtcaacgg cggcgacggc
     2821 aacgacacgc tcgaagcatc ggcaaacttt aacgaggacg tctgccacat gttcggcgac
     2881 aatggcaacg acacgcttca ctcggtcagc gccggcgcgg gcgggaactt gctggcaatt
     2941 atgtccggcg gcaatggcaa cgacacgttc cgcaacgacg atatcgacaa caggatcaac
     3001 gccaccggcg ggccgggtat cgacacgatc cagctgggcg agggctcgag cggcatcatc
     3061 gcgttggatg gcgtcgacca cggcagcaat accatcgccc atgccgacgt cgagaacgtc
     3121 gtcggatcgc cctttggcga cgtcgatatc atcggcaatg ccttgaacaa tctgatcgac
     3181 gtcgggagcg actcggccgt gactgttttg ggcggcggag gcaacgacac gatcaccgcc
     3241 agcggcgata cgacttacct cgacggtgga agcgggaacg acgtcatcac ctgtgacagc
     3301 acccaacccg gcaccacgct gatcggcggc gacggcaacg acacgctgat cggcggcgac
     3361 gcgcccgaca aactcttcgg cggcaacggc aacgaccgcc tcgtcggcaa cgccggcagc
     3421 gacacgatg
//
LOCUS       DATIMH010000916         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1086748, whole genome shotgun sequence.
ACCESSION   DATIMH010000916 DATIMH010000000
VERSION     DATIMH010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086748"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VLI90_15935"
     CDS             <1..213
                     /locus_tag="VLI90_15935"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSV15752.1"
                     /translation="TAGDTVDEALTMAKEAIALHLTGMLDDGLELPEPTEDIDRLRAD
                     PDYTDAQLWAAVQVDLDWTRSSSTAA"
     gene            complement(219..2711)
                     /locus_tag="VLI90_15940"
     CDS             complement(219..2711)
                     /locus_tag="VLI90_15940"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HSV15753.1"
                     /translation="MPASTVAKRKSKSVVGDGAAGAGGNGSSNGAGSGTTGGGGEERA
                     PLHEIAQTRYLNYALSVITSRALPDVRDGMKPVQRRIIYTMWQNGLRADQKHRKCATV
                     VGDVMGRYHPHGDSSIYEALVRMAQPFMMRATLVDGSGNFGSMDGDPAAAMRYTECRL
                     TPIAAETISEIGQGTVHFRPNYDGTKEEPVVLPSRIPNLLLNGSTGIAVGMATSIPPH
                     NLGELCAALLKLADDPDIKEYQLIGNDAVRGPDFPTGGQVLNSKSELLEIYKTGQGAI
                     RMRATWEKGQATRGTQIIYITSIPYGVNKAELVEAIAQVVLSRKMPLLLDVKDVSTDD
                     VRIELQLKRDAEIDKVMAFLFKHTLLQTNFNVNLTCLTPTENPEVGKPERLGIKQILW
                     HFLHFRLDVVTKRLTHELAQQRKRIHTLEGFAFAFDILDDLIKIIRGSDGKADAADRI
                     LKKYGVAPHGPRKPKDDGLDPDQVEDILELKLYRLAKLEINLIREELAERNKRAKEIR
                     RLLDEEEASGRWGIVRSEIEELAKTYGKDPKNKRRTIIKDAENEVEYSAEDFIVAEDN
                     HVLVTADGWVKRQKEINPETTRLREGDRILALVAGSTRATITFFSNFGTAYSARIIDI
                     PATTGYGEPIQKLFKLKDGERIVAAASMDERVLPLKATKVDEKKPDVAPKLHGIAITS
                     DGYSLRFGLDTFREASTRAGRRFARPAQGQEVIFAAVITGDETIIAASQGRRVMVCKA
                     DEINFLSGPGKGVLLIKLDKTDKLLAAITATSDRDTLTVKTSMGGEQRLNTGRYRVTG
                     RGGKGNEFVSRGQIVEVMHEPVPAPAALDGKA"
BASE COUNT          476 a          959 c          871 g          647 t
ORIGIN      
        1 acggcgggcg acacagtcga cgaagcgctg accatggcta aagaagcgat cgcgcttcat
       61 ctgaccggaa tgctcgacga cggtctggag ctaccggagc ccaccgaaga catcgatcga
      121 cttcgggccg acccagacta cacggacgct cagctttggg ctgccgttca ggttgatctg
      181 gattggacac ggtcatcctc caccgcggcg tgatctgctc acgccttgcc gtccaacgcc
      241 gccggggccg gcaccggttc gtgcatcact tccacaatct gcccgcgcga aacgaactcg
      301 ttgcctttgc cgccgcggcc ggtgactcgg taccgcccgg tgttcaggcg ttgctcgccg
      361 cccatgctgg tcttcaccgt cagcgtgtcg cgatcgctgg tcgcggtgat ggcggccagc
      421 agtttgtccg tcttatccag cttgatcagc agcacgcctt tgccggggcc gcttaagaag
      481 ttgatctcat ccgccttgca gaccatgacc cggcggccct ggctggcggc gatgatcgtt
      541 tcgtcgccag tgatgacggc ggcgaagatc acttcctgcc cctgcgcggg ccgcgcgaag
      601 cggcggccgg cgcgagtcga tgcttcgcgg aacgtatcga gcccgaaccg cagcgaatag
      661 ccgtcgctgg tgatggcgat accgtgcagc ttcggcgcga cgtccggttt cttctcgtcc
      721 accttggtcg ccttcagcgg cagcacgcgt tcgtccatcg acgcggcggc gacgatgcgc
      781 tcgccgtcct tcagcttgaa cagcttttga atcggctcgc catatccagt cgtcgcgggg
      841 atgtcgatga tgcgcgcgct gtacgcggtg ccgaaattgc tgaagaacgt gatcgtcgcg
      901 cgcgtgctgc cggcaaccag cgccaggatg cggtcgcctt cgcgcagccg cgttgtctcg
      961 gggttgattt ccttctgccg cttcacccaa ccgtcggccg tcaccagcac gtggttgtcc
     1021 tcggcgacga tgaaatcttc cgccgagtat tccacttcgt tctcggcgtc cttgatgatc
     1081 gtgcggcgct tgttcttcgg gtccttgccg taagtcttgg ccagttcctc gatctcgctg
     1141 cgcacgatgc cccatcgccc cgatgcctct tcttcatcca gcagccggcg gatttccttt
     1201 gcccgcttgt tccgctcggc cagctcctcg cggatcaaat tgatctcgag cttggccaac
     1261 cgatacagct tcaattccag aatgtcttcg acctgatcgg gatcgagccc gtcatccttc
     1321 ggcttgcgcg gcccgtgcgg ggcgacgccg tacttcttca aaatgcgatc ggccgcgtcg
     1381 gccttgccgt cgctgccgcg gatgatcttg atcaggtcat ccagaatgtc gaaggcgaac
     1441 gcaaaacctt ccagcgtgtg gatgcgcttg cgctgctggg ctaattcatg cgtcagccgc
     1501 ttggtcacca cgtccaggcg aaagtgcaga aagtgccaga gaatctgctt gatgccgagc
     1561 cgttcgggct tgcccacttc ggggttttcc gtcggcgtca ggcaggtgag gttgacgttg
     1621 aagtttgtct gcagcagcgt gtgcttgaac aggaacgcca tcaccttgtc gatctcggcg
     1681 tcgcgcttca gctgcagctc gatgcgcacg tcatccgtgc tgacatcttt gacgtccagc
     1741 agcagcggca tctttcggct gagcaccacc tgcgcgatcg cctcaaccag ctcggccttg
     1801 ttcacgccgt agggaatgct ggtgatgtag atgatttgcg tgccgcgcgt cgcctggcct
     1861 ttttcccagg tggcgcgcat gcggatcgcg ccttggccgg tcttgtagat ctcgagcagc
     1921 tcgctcttgc tattcaggac ttgcccgccg gtgggaaagt cagggccgcg caccgcgtcg
     1981 tttccgatga gctggtattc cttgatgtcc ggatcgtccg ccagcttcag cagcgccgcg
     2041 cacaactcgc ccaggttatg cggcggaatg ctcgtcgcca tgccgacggc aatgcccgtc
     2101 gatccattca gcagcagatt tggaatgcga ctcggcagga cgaccggctc ttccttcgtg
     2161 ccgtcatagt tcggccggaa gtgaactgtc ccctgcccga tttccgaaat cgtctccgcc
     2221 gcgatcggtg tcagccggca ttcggtgtat cgcatggccg cggcggggtc gccgtccatc
     2281 gatccgaagt tgccgctgcc gtccaccagc gtcgcgcgca tcatgaacgg ctgcgccatg
     2341 cggaccagcg cttcatagat agagctatcg ccgtgcgggt gatatcggcc catcacatcg
     2401 ccgaccacgg ttgcgcactt gcggtgcttc tggtcggcgc gcaggccgtt ctgccacatc
     2461 gtgtagatga ttcgccgctg cactggcttc ataccgtcgc gcacgtccgg cagcgcgcgg
     2521 ctggtgatga ccgacagcgc gtaattcaga taccgcgtct gggcgatctc atgcagcggc
     2581 gcgcgctctt ccccgccgcc acccgtcgtg cccgaacccg cgccgtttga tgagccattg
     2641 cccccggcgc cggccgcgcc atcgccgacc actgatttgc tttttcgctt agccacggtt
     2701 gatgcgggca tagatacttc cgcccgcccg ccaggtccat ccaagccgtc gtgtgccatc
     2761 cgtggaatcc tcctgcactg cccgagtcaa cttaccgttg gctttatcgg acgaagccca
     2821 tttccgcctt aagcctttgg ctaacgtgtt aggtcattgt acggatcata cccgtgtttt
     2881 tcaatcgcta aacaggaaaa ataactgaag gtgacgtgga aacaaaggac gcagtgacag
     2941 gcatttagga aaa
//
LOCUS       DATIMH010000917         4046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1957294, whole genome shotgun sequence.
ACCESSION   DATIMH010000917 DATIMH010000000
VERSION     DATIMH010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957294"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VLI90_15945"
     CDS             <1..1107
                     /locus_tag="VLI90_15945"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSV15754.1"
                     /translation="RAGVKYAVGRESKAEFGEEAAGIRFDKAPRKARTVDESGNLLPG
                     ISAWAKDLKEGDAHPGVMNYNFRLTVAKDPKFQAPIPAPKNYDAKRYALLAGWFAGTA
                     ARNEPSKLTDVVDLYARRNGKFELNNKQAAIISLGDFGAQFGWPEGDYAARRRIFDDH
                     WDYTLGLLQFLATDAAVPSNVRAEMKSLGLHTGEFADNGYLPYQLYVREARRMRGDYV
                     MTQKDVQDDRRKSDAIGMSSHFIDAHHVQRVALSDSEFVNEGRIWRMGYAYQIPYRAL
                     TPKAAECANLLVPGAASYSHVAFCTLRLESVWMIAGHAAGVAAAMMVKGKTKGVQELD
                     VRQLQAKLREQKQVIEFATGQPEKCEKLNGPAEF"
     gene            complement(2255..3478)
                     /locus_tag="VLI90_15950"
     CDS             complement(2255..3478)
                     /locus_tag="VLI90_15950"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSV15755.1"
                     /translation="MGLVVQKFGGSSIATPERIHIAARRAVAVKAAGHQVVVVVSAMG
                     DTTDDLIELARKVCDNPPKREMDQLLATGEQVTIALMAMALHAQGHEAISFTGGQIGL
                     VTDNAFSKARIQSINKQRIFEQLSAGKIVIVAGFQGVTPEGDYTTLGRGGSNATLVAL
                     GAVLQADVCENYTDVDGIYTADPRIVKNARKIERISYDEMLELSGLGASVLQTRAVEF
                     AKKYNVPLHVRNSQNESTGTWIVAETPNMEHIVVSGAALKQDLTRVTIKSVPDRPGIA
                     ARIFGDIAAANIVVDDIIQTVSDDNTANISFTVEHGDLHDIKPVVDRLTKELGAKTHP
                     IYQGDLAKVSVVGVGMRVHTGVAQRMFKTLADAKINIQNITTSEIKISVIINKGDGPK
                     ALQLVHDAFELDKAK"
     gene            complement(3592..>4046)
                     /locus_tag="VLI90_15955"
     CDS             complement(3592..>4046)
                     /locus_tag="VLI90_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15756.1"
                     /translation="IRAPFVVDCSPFQHAGRWWMFANGGPPENNNTLDLYFAPELRGP
                     WTPHPQSPLNTSRAEARPAGRVIQFGARIYRLGQDCEKAYGTAVRAYEVTRLSETEYA
                     ETRIDPPLLGPTGEGWNANGMHHMDCAQLQDGSFIAAVDGWAYSAPEE"
BASE COUNT          775 a         1119 c         1349 g          803 t
ORIGIN      
        1 cgcgccgggg tgaaatacgc cgtcggtcgc gagtcgaaag cagagttcgg cgaagaagct
       61 gccggcatcc gcttcgacaa ggctccgcga aaggcacgaa cggttgacga atcgggcaac
      121 ctcctgccgg ggatcagcgc atgggccaaa gacctgaagg agggggacgc gcatccggga
      181 gtgatgaact acaatttccg gctcaccgtc gccaaggacc ccaagtttca agcgccgatc
      241 cccgcaccaa agaattatga cgcgaagcgg tacgcgctgt tggccgggtg gtttgccgga
      301 acagcggctc ggaacgagcc atcgaagctg accgacgtgg tcgatctcta tgcgaggcgc
      361 aacgggaaat tcgagctgaa caacaagcag gccgccatca tctcattggg cgatttcggc
      421 gcgcagttcg gctggcccga aggggattac gcggcgcgcc gccggatctt cgacgatcat
      481 tgggattaca ccctgggtct gctgcagttc ctggcgaccg atgccgcagt gccatcgaat
      541 gtgcgggcgg aaatgaaatc gctggggctt cataccggcg agtttgccga caacggctat
      601 cttccctacc agctctacgt tcgcgaagca cgccggatgc gcggcgacta tgtgatgacg
      661 cagaaggacg tgcaggacga tcgacgaaag agcgacgcga tcggcatgag cagccatttc
      721 atcgacgctc atcacgtaca gcgcgtggcg ctcagcgaca gcgaattcgt caacgagggc
      781 cgcatctggc ggatggggta cgcgtatcag atcccgtacc gggcgctgac gcccaaagcg
      841 gccgaatgcg ccaacctgct cgtcccgggg gcggccagct actcccacgt cgcgttctgc
      901 acgctgcgat tggagagcgt ctggatgatc gcgggccacg ccgcgggtgt cgccgccgcc
      961 atgatggtaa agggcaaaac caagggcgta caggaactcg atgtgcggca gttgcaggcg
     1021 aagcttcggg agcagaagca ggtgatcgaa tttgcgacgg gccagccgga gaagtgcgag
     1081 aagctcaacg ggccggcgga gttttagccc gtcgtcaggg cacgacgtaa agcacgaccg
     1141 tcgctcgggg cgggactttg agcgacccag tgagtgcgat gctttggccg gtcgcgatat
     1201 cgattgcgtt gcccacgttc gcaacgcgat tgtcctttcc aattctggac cgcatagtcg
     1261 atggggcagc tattctctca gccgaacttg gatgaacttg gggttcaatg gattcaagat
     1321 cagcaacccg ttcagcagta tttatttggt tgaagttccc ttgtggttcg tagcacttgt
     1381 tttgttcgca tggccactgt ggatgatctg gacgtcggtc tccaagaggc gtgccgatcg
     1441 tggttttgaa atttgaaagt tcgacgacag aaggtaacgc cggccgtcgc gttcgatgct
     1501 ggcaccgtaa accttgtaat gaaacgaact tgtgggcgat gttacacggg ggtgccggcg
     1561 ggcggcgcgg aaagggacaa ttccggggcc tgtcaggcga gtaagtgact tgaagaacaa
     1621 gagttaagga ttccggctcg ggtggtggcc aatcgacatc agatgttaca tctcgggagg
     1681 gtgtaacatg ttctacgggg ccattgaaga tctaaggtgc ttatcatcaa gcatttacat
     1741 attcattgaa ttggatcgga tgttacatcg gcacaaagtc atgggtcgcc gagacttgga
     1801 ggccgcaccc tgcggagaaa cgcattcgtg aagagatgca aagcgaaggt gcggagtcgc
     1861 gaagagaaca caaaccggat gcgccgccaa ggtcgccaag agcgccaaga agatgctgaa
     1921 ggaaggcgaa gaaaatggga ttcgggatta cgccttcgcg gtaatccgga tttgaagccg
     1981 ttgctgactc aggcgcgcgt gtgcggggcg aaaagatcat caaagggcgc gaatttggcg
     2041 ccaaaaagtg aaaaatcgag aagggcgcgc cccatttttc gtttaaatgg tgcgccgtgg
     2101 ggcgcgggaa gggcagcagt gagagcgggt gggactccaa agcgggacag tgtgagacaa
     2161 gtgagacatt cgaagggccg gctgggttga aaaagtgcac agttttgcac agttctgcac
     2221 ggctccatcc tccatctgta tccgtgcggc gggcttactt ggccttgtcg agttcgaacg
     2281 catcatgcac cagttgcagg gcctttgggc cgtcgccttt gttgatgatg acgctgatct
     2341 tgatctcgct ggtggtgatg ttctggatgt tgattttggc gtcggcgagg gttttgaaca
     2401 tgcgttgggc gacgccggtg tggacgcgca tgccgacgcc gaccacgctg acctttgcca
     2461 gatcgccctg gtagatgggg tgggtcttgg cgccgagttc tttggtcagg cgatcgacga
     2521 cgggtttgat gtcgtgcagg tcgccgtgct cgacggtgaa gctgatgttg gcggtgttgt
     2581 cgtcggagac ggtctggatg atgtcgtcca cgacgatgtt ggcggcggcg atgtcgccga
     2641 agatccgggc ggcgatcccg ggccggtccg gcacgctctt gatcgtcacg cgggtcaggt
     2701 cctgcttcaa cgcggcgccg gatacgacga tgtgttccat gttgggagtc tccgcgacga
     2761 tccacgttcc ggtgctttcg ttctggctgt ttcgcacgtg cagcggcacg ttgtacttct
     2821 tggcaaactc aaccgcgcgc gtctgcaaca ccgacgcgcc cagccccgag agctcgagca
     2881 tctcgtcgta gctgatccgc tcgatcttgc gggcgttctt cacgatccgc gggtcggcgg
     2941 tgtagatgcc gtccacgtct gtgtagtttt cacacacgtc tgcctgcagg acggctccga
     3001 gcgccaccag cgtggcgttg ctgccgccac ggcccagggt ggtgtagtcg ccctccggcg
     3061 tcacgccctg aaaccccgcg acgatcacga tctttcccgc gctgagctgc tcgaagatgc
     3121 gctgcttgtt gatcgactgg atgcgggcct tggagaaggc gttgtcggtg acgagcccga
     3181 tctggccgcc ggtgaagctg atggcttcgt ggccctgggc gtggagcgcc attgccatga
     3241 gggcgatggt gacctgctcg ccggtggcga gcagttggtc catctcgcgc ttcggggggt
     3301 tgtcgcacac cttcctggcc agctcgatca agtcgtcggt ggtgtcgccc atggcactga
     3361 cgacgacgac cacctggtgt cccgcggcct tgaccgccac cgcccgccgg gcggcgatgt
     3421 ggatccgctc cggtgtggca atgctcgacc cgccgaattt ctgtacgact aagcccatac
     3481 gttctgcttc ccgctccgca tgcgtggaaa agggcgggat tatagtggga tcggcggggc
     3541 gctcaactgt tggtcggggg aagaggcacg aagacccagc cgacaggcaa atcactcctc
     3601 gggcgcagag taagcccacc cgtccactgc ggcgatgaag gagccgtctt gcaactgcgc
     3661 gcaatccatg tggtgcatcc cattcgcgtt ccaaccctca cccgtcggcc cgagcagcgg
     3721 cggatcgatg cgtgtttccg cgtactcggt ttcactgagg cgcgtcactt cgtacgcgcg
     3781 gaccgcggtg ccgtaggcct tttcgcagtc ttgaccgagg cggtaaatcc tggcgccgaa
     3841 ctgaatcacc cgtcccgccg ggcgggcctc ggcccggctg gtgttcaagg gcgattgggg
     3901 gtgcggggtc caggggccgc gaagctccgg ggcgaagtag aggtcgagcg tgttgttgtt
     3961 ctccggcggg ccgccgttgg cgaacatcca ccagcggccc gcatgctgaa acggggagca
     4021 gtccacgaca aaaggcgcgc ggatca
//
LOCUS       DATIMH010000918         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2529042, whole genome shotgun sequence.
ACCESSION   DATIMH010000918 DATIMH010000000
VERSION     DATIMH010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529042"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VLI90_15960"
     CDS             complement(<1..365)
                     /locus_tag="VLI90_15960"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HSV15757.1"
                     /translation="MSGRPCVDQSDISQRRRSRRRPRRLALMENLELRRLLSAIPLPN
                     IPNRTFNITNYGAVADGVTDNTAAIQSAINAAVAAGGGKVEVPAAAKPFESGPLTLKN
                     YINLQVDAGATLQMLPYGT"
     gene            complement(581..1216)
                     /gene="nth"
                     /locus_tag="VLI90_15965"
     CDS             complement(581..1216)
                     /gene="nth"
                     /locus_tag="VLI90_15965"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSV15758.1"
                     /translation="MRRLRTAVKPYTRAAMFQLADEGHRSLFEQLVACIISIRTMEEV
                     TLPTCRRLFIAARTPETIAKLSVSQIDELIRGCSFHEPKAKTIRDIAIRAVKEFAGEL
                     PCDFEELTSFHGVGPKCANLALGIACDEPHGVPVDIHVHRVTNRWGYVAAPTPEQTMV
                     QLEAKLPKKYWMEINRLLVPFGKFACTGKMPKCSTCPLLEMCRQVGVTSSR"
     gene            complement(1343..1636)
                     /locus_tag="VLI90_15970"
     CDS             complement(1343..1636)
                     /locus_tag="VLI90_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15759.1"
                     /translation="MSSVRFRLILLFAIIALTASIAPACEMCRDAVITNGGGGGIDGT
                     ASSAGLNFNSSIFFMLGGVFAVAGWIGWIMYKAIQSSQATPAPRGFPVKSNVG"
     gene            complement(1871..2437)
                     /locus_tag="VLI90_15975"
     CDS             complement(1871..2437)
                     /locus_tag="VLI90_15975"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HSV15760.1"
                     /translation="MQPIDVQIPAFRATSNAKAVAVILAISAAVFGFLIWLIYVKPAA
                     GHTSNLISALPAVNASLNALSTVFLVAGYVEIRNRRFTRHMTLMFAALASSTLFFICY
                     VIYHNFHGDTRFLNHDPLIRPAYFFVLITHIVLSGVAVPLILTSFYLALAGKYALHRR
                     VSRYTFPIWLYVSVTGVLVFAMLKLFNG"
     gene            complement(2520..3155)
                     /locus_tag="VLI90_15980"
     CDS             complement(2520..3155)
                     /locus_tag="VLI90_15980"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HSV15761.1"
                     /translation="MDKRVKRIVIVMWAVTAVAAAALGGAVWMMRSAGGSGPAISAAE
                     SPTAGLPVLFDAPSFSLTDQNGQSFDSAGLHGKVWVADFIFTSCAGICPMMTQHMRAF
                     QKLTPDSPVQIVSFSVDPGHDTPAVLKQYAEAAKADEARWHFLTGESRAKLWDICKAM
                     KLAVGPDTNNQVMHSNQFLLIDGDGHVRGVYNSEDDSFLPKLVADANTLTK"
     gene            complement(3169..>3938)
                     /locus_tag="VLI90_15985"
     CDS             complement(3169..>3938)
                     /locus_tag="VLI90_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV15762.1"
                     /translation="CRRELVRFLRQRHRIIGALATPIVFWLLIGGGMSHSFKSDASTN
                     GGGYMEYFFPGTILMILLFTAIFSTISIIEDRREGFLQSVLVAPVSRLSIVLGKVLGG
                     TVLAFGQAFIFLLLAPTVGIHMTLVGFLASLLMMLILAFALTALGFCIAWPMSSTQGF
                     HAIMNLFLMPMWFLSGALFPPGGAAGFIRAVMMINPLTYGMEALRRAMYWGSPSYATS
                     SHMPGFAVCAGITVAFAAALMLLASVIAQKRTAADLQ"
BASE COUNT          756 a         1178 c         1300 g          704 t
ORIGIN      
        1 tacgtgccgt atggcaacat ctgcaacgtc gcgccagcat ccacctgcag gttgatgtaa
       61 ttcttgagcg tgagcggccc gctctcaaac ggtttggcgg cggcaggcac ctccaccttc
      121 ccaccacccg ccgcgacggc ggcattgatc gccgactgaa tggcagcggt gttgtccgtg
      181 acaccgtcgg cgaccgcgcc gtagttggtg atgttgaacg tgcgattcgg aatgttcggc
      241 agcgggatgg ccgagagcag tcggcgcagc tccaggtttt ccatcagcgc caggcggcgc
      301 gggcgccggc gcgaccgacg acgctgcgag atgtcggatt gatcgacaca ggggcgaccg
      361 ctcattggct cctcatccgc gcctgcccgg gcttggttgt aatcgatcgc gacgcgtccg
      421 cgtgtgcgtg tcggtgtggg gcaagatggc gacgcgggca agtataaacc ggccgctttt
      481 cggaactggg ccaaagtttt tgggaatcgg accgaaggtt tgattcatgc acgtgccgct
      541 tctatcgctg gcgtcgcggg gcgtgaacat ttcgccgggg tcatctgctg ctcgtcacgc
      601 cgacttgccg gcacatctcc agcagcgggc acgtcgagca tttgggcatc ttccccgtgc
      661 aggcgaactt gccgaacggc accagcaggc ggttgatctc catccaatat tttttcggca
      721 gcttcgcttc cagctgcacc atcgtctgct ccggcgttgg cgcggcgacg tagccccaac
      781 ggttggtgac acggtggacg tggatgtcca ctggcacgcc gtgcggctcg tcgcaggcga
      841 tgccgagcgc gaggttggcg cacttgggac cgacgccgtg gaagctcgtc aattcctcga
      901 agtcgcacgg cagttcgccg gcgaattctt tcaccgcgcg gatcgcaatg tcgcggatcg
      961 tcttcgcctt cggctcgtga aacgagcacc cgcggatcag ctcatcgatc tggctcaccg
     1021 ataactttgc gatcgtctcc ggcgtgcgcg ccgcgatgaa cagccggcgg caggtgggca
     1081 gcgtgacctc ctccatcgtg cggatcgaga tgatgcacgc gaccagttgt tcgaacagcg
     1141 agcgatgtcc ttcgtccgcc agctgaaaca tggcggcccg cgtgtagggt ttgaccgcgg
     1201 tgcgcaggcg gcgcatcacg aggtcgatct cgaagggccg tttgagcgag cgatgaacgg
     1261 gcgtggccgt cggcctccgt tgccggatat ttgtgtgcgt ccctgccaca gcaacatcaa
     1321 atgttacgaa gggcgctggc gatcagccaa cgttcgattt gaccggaaac ccccgcggag
     1381 cgggcgtagc ttgtgagctt tggatggctt tgtacatgat ccagccgatc caccccgcga
     1441 cggcgaagac gccgcccagc atgaagaaga tgctggagtt gaagttgagc ccggcggatg
     1501 aggcggtgcc gtcaatccca ccgccaccgc cgttggtgat gaccgcatcg cgacacatct
     1561 cgcaggcggg cgcgattgac gccgtcagcg cgatgatggc gaacaggagg atcaggcgga
     1621 agcgcacaga ggacatgacg gccgttatcg taatccggcg gcggtggcgg atgcaaggga
     1681 aaccctggac caacccacac ttttgcagaa gtggcctgaa acccactcac gccgggttcg
     1741 gaggtactcc cacgaccgcg atcttgccgc gcgagaagaa tctcgcttcg tcgcgagtac
     1801 gctccgaacc cggcgtggct acgttcgacg tgcgggcatt ggccgtggtc gatgaagcct
     1861 tcagccaccc tcagccgttg aataatttca acatcgcaaa caccagcacg ccggtgaccg
     1921 agacgtagag ccagatcgga aatgtgtatc gcgatacccg gcggtgcagg gcatatttgc
     1981 cggccagggc gagataaaag ctcgtgagga tcagcggcac cgcgacgccc gacagtacga
     2041 tgtgggtgat caggacgaaa aagtatgcgg ggcggatgag cgggtcgtgg ttgaggaatc
     2101 gcgtgtcgcc gtggaagttg tgatagatga cgtagcagat gaagaagagc gtggagctgg
     2161 cgagggcggc gaacatcagc gtcatgtgcc gggtgaaccg gcggttgcgg atttcgacgt
     2221 aaccggcgac gaggaagacg gtgctgagcg cgttgagcga cgcgttgacg gcggggaggg
     2281 cgctgatcag atttgacgtg tgacctgcgg cgggcttcac gtaaatcaac cagatgagga
     2341 acccgaaaac cgcggcgctg atggcgagaa tcacggcaac ggctttggcg ttgctcgtgg
     2401 cgcgaaaggc gggaatttgg acgtcgattg gctgcatctg ttgcacgggc tacgagcctt
     2461 cggctctctc gcccgtgtcc aagcacctcg gcacgggcga gacgcgcgag acgcccgtgc
     2521 tactttgtta gcgtattcgc atccgcgacg agtttcggca ggaacgaatc atcttcgctg
     2581 ttgtacacgc cgcgcacatg gccgtcgcca tcgatcagca ggaactgatt gctgtgcatg
     2641 acctggttgt tcgtgtccgg gcccacggcc agcttcatcg ctttgcagat gtcccacagc
     2701 tttgcccgcg attcacccgt gagaaaatgc catcgcgctt catccgcctt cgcggcctcg
     2761 gcgtactgct tcagcactgc gggcgtgtcg tgcccgggat cgaccgagaa cgagacgatt
     2821 tgcaccggcg agtctggcgt gagcttttga aacgcccgca tgtgctgcgt catcatcggg
     2881 cagatgcccg cgcagctggt gaagatgaag tcggccaccc acaccttgcc gtgcaggccc
     2941 gcgctgtcga acgattggcc gttctggtcg gttagcgaaa acgacggcgc gtcgaacaag
     3001 acgggcaggc cggccgtcgg agactcggcg gcggatatcg ccggtccgct gccgccggcg
     3061 ctgcgcatca tccacacagc gccaccgagc gccgcggccg ccacggcggt gaccgcccac
     3121 atcacgatca cgattcgctt tacacgcttg tccatcaggt ctgtgattct actgcaaatc
     3181 cgccgcggtg cgcttctggg cgatcacgct cgccagcagc atcagtgctg ccgcgaaggc
     3241 gacggtgatg ccggcgcata ccgcaaagcc cggcatgtgc gatgacgtcg cgtacgacgg
     3301 cgagccccag tacatcgccc gccgcagcgc ttccatgccg taggtcagcg ggttgatcat
     3361 catgacggcg cggataaatc ccgcggcgcc accgggtgga aacagcgcgc cggacaggaa
     3421 ccacatcggc atcaggaaca agttcatgat ggcgtggaag ccttgtgtgc tgctcatcgg
     3481 ccaggcgatg caaaagccga gcgccgtcag cgcgaaggcg aggatcagca tcatcagcag
     3541 cgaggcgagg aatccgacca gcgtcatgtg aatgccgacg gtgggggcga gcaggaggaa
     3601 gatgaaggcc tgcccgaagg ccagcacagt gccgccgagc actttgccga gcacgatcga
     3661 caggcgcgac accggcgcga ccagcaccga ttgcaaaaag ccttcccggc ggtcttcgat
     3721 gatcgagatc gtcgagaaga tcgcggtgaa cagcaggatc atgaggatcg tgccggggaa
     3781 gaaatattcc atgtacccgc cgccgttggt cgaggcgtcg gacttgaagc tgtggctcat
     3841 cccgccgccg atcagcaacc agaacacgat cggcgtggcg agcgcgccga tgatgcggtg
     3901 gcgctgccgc aggaagcgaa cgagttcccg ccggcaaa
//
LOCUS       DATIMH010000919         7408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3108453, whole genome shotgun sequence.
ACCESSION   DATIMH010000919 DATIMH010000000
VERSION     DATIMH010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3108453"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..489)
                     /locus_tag="VLI90_15990"
     CDS             complement(160..489)
                     /locus_tag="VLI90_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15763.1"
                     /translation="MSLPPTFGEQWARWIPSLSWSSQPNRATFGAQENRIMVGNHAES
                     LRKAIEGLIDAKLHDAMARPGGLDRLMAHRRTGVASYDIRQAERRLQDTLADVLSAQP
                     EDNYQSA"
     regulatory      complement(564..805)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1125..2255
                     /gene="tgt"
                     /locus_tag="VLI90_15995"
     CDS             1125..2255
                     /gene="tgt"
                     /locus_tag="VLI90_15995"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSV15764.1"
                     /translation="MAALTFEITTTDGSTGARTGRVTTRHGRFDTPAFMPVGTQGSIK
                     GLLPDHVAATGAQCILANTYHLMLRPGERVVAQLGDLHAFMGWPGPILTDSGGFQVFS
                     LSDINKISDDGVTFKSHVDGSMVHLTPRRSMEVQNALGADIMMAFDECPPADAPVEYH
                     EEAVARTLRWAAECVEAHARPDDQSLFGIVQGGVHEQLRALCAEKLVEMDLPGYAVGG
                     LAVGEGFEAMKAILGFTTPLLPVNKPRYLMGVGFPRDIVAAVARGIDMFDCVMPTRNG
                     RNAYAFTMDGAIRLRNSRFTEDKGPIETGCDCYACRTFTRGAIRHFFFAGEMLGPILL
                     SLHNIRFYQRLMADIRGSIREGRFESFYRDDPRSRMGPSEVA"
     gene            2345..2704
                     /gene="yajC"
                     /locus_tag="VLI90_16000"
     CDS             2345..2704
                     /gene="yajC"
                     /locus_tag="VLI90_16000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSV15765.1"
                     /translation="MNSIIAFLAQAAPAAPTSQPVPGWARFITQSGPLLPLAIGFIFL
                     MMFMNRSKKKQEHSRDEMLKQLKRGDRITTIGGIHGTVLRTEDSRVEVKVDEGNNTKI
                     WFSRSAIHKVAEEEKAV"
     gene            2787..6200
                     /gene="secD"
                     /locus_tag="VLI90_16005"
     CDS             2787..6200
                     /gene="secD"
                     /locus_tag="VLI90_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSV15766.1"
                     /translation="MEQNQTGRVIFILAVLLAAVLSIVPQPLKLFRHDVPLGEKLNLK
                     PGIDMVGGTSLLYQIKTPEGGFHGNNGQTLAEAVMESLKRRVDPNGVRNLIWRPQGTD
                     RLEIQLPASPNSEEAKKAREAYVAAQQQLSDTNVSSTEVINAVEQLKGDERRDRLNQL
                     AAGSDARAKLFGALASAYDQKQAAHEKRDAELEAQKTEEYDKLKSQVEQSNLAVGQLE
                     AMLDAPADQRTAKLAELKKQYADYPARVSAIDNFVKSYDEYNKLKGSIDDAAQLKREL
                     QGSGVLEFHILANDLPRAEYIAMVDRLKSKGPRVEAGDVVKWFMVDRPQDVQSFGNEV
                     QEWNGKYYALCYITDDKSMVNGPHTNHWTLESAHPSSDGEGGRAVGFTFDAVGGDYFS
                     TFTQNNLKKPLAAILDNKIVSVANINSRIGREGVITRGNGYDDEEFNYLISTLNAGSL
                     PATLADEPISEHTVGPQLGEDNLRRGLMACGFGLIIVAVFLVGYYYLAGVVATFAVFM
                     NVILILGILAAFNATFTLPGIAGIVLTIGAAVDANVLIFERLREEQHHGLSIRMAMRN
                     AYLHARGAIWDSNATTIITSAILIWLGSEEVKGFGLTLLIGLASSLFTSLFVTRTIFN
                     IMIDKFGYQHFGSLPLTFPKWDQFLKPNIDWMKIVPYCLTFSAVFIALGMTAFVMKAR
                     AHELADIDFASGTQVQFELKDRTKIEDLRSMFTKADNPALPSPSLVSVNHDDLTYEIV
                     TPNVDAKAVREAVLSVVGTKIKTDLPSKFDHVDESMDQAMGSAIQPIVKTPLVINGFT
                     VPRAENYLGGAAIVLNHLNPPLKVSEIKTRIDQMRVQDESAQSGAEGSRYHDFTVVSP
                     NGPDQPTSTAIVLTGDPNLPYDKDEGKWREELVAPMWKLINQSINRPATLQKVNNFDA
                     AVAGDTQKAAFYSLVLSSLVIMFYIWFRFGNLKYGTATVLAMIHDTFLVVGAIGLSHW
                     MVQYTPGLARILLVEPFRINLTVVAAVLTVMSYSMIDTIVVFDRIRENRGKFGHMSRK
                     IINDSINQTLSRTLLTCGITIMTVASMYFVGGPGVHGFTFVLLVGILVGTYSSIAVAA
                     PILLMGHEAKGDSGGSGGAGWAAAKNPAPTPGKTERAVAVAVNKVKTAG"
     gene            6306..7262
                     /locus_tag="VLI90_16010"
     CDS             6306..7262
                     /locus_tag="VLI90_16010"
                     /inference="COORDINATES: protein motif:HMM:NF017316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSV15767.1"
                     /translation="MRKDAKIGFAIGGVLLAVLTVYAIVVPKHNRTPNAAGGVTLVTP
                     TTAAPSIQSPATTGNDLPPAVAGALNGDRRADPKPEPAVATNDTNKSSDGVNWAKLLS
                     GSPDAPTLMSTTPSSSDDATPAPAASAKETQVAKATPTPSADAASDPAPETTPLMAKA
                     NISTPATQPLATGRTYTIKPGQTLSSVAAEVYGNQRFYVAILRANPTVNPARLRPGTK
                     VTLPDISDVQPKADAPVLAATAKQTQSESTEKASSGHTYKVESGDNLYRIARKLYGSP
                     KLAETIYELNKDRIGPDKTKLKLGMVLDLPQSPSASATASSR"
BASE COUNT         1497 a         2182 c         2390 g         1339 t
ORIGIN      
        1 ccgggccgtg gcgatcgtta gagtcggggg atgtttctac gcggtccgca cgcgggccgg
       61 cgggatggct cacttgctag ggcgttgcca tcgtcaaacc cgcggggcgg agcgtcttca
      121 tccaccgagg agccaaaaga tcgttcaagc gccgccgtat taggcgctct ggtagttgtc
      181 ctccggctga gcgctgagca cgtcagccaa ggtgtcttgc agccggcgtt cggcttggcg
      241 gatgtcgtag gaggcgacgc cggtgcggcg gtgagccatc aggcggtcga gaccacccgg
      301 gcgcgccatg gcgtcgtgaa gcttggcatc gattagccct tcgatggcct tgcgaagtga
      361 ttctgcatgg ttaccgacca tgatacggtt ctcctgtgcg ccaaaagtgg cgcggttagg
      421 ctgtgaagac caagaaagcg acggaatcca ccgagcccat tgctcgccaa acgtcggcgg
      481 caacgacatc ccggtggcgc cacgatggcg cgacacagcg gacagaggaa atcaggcagg
      541 agtcaagcgc ggggcgggcc gcgttgatcc ctttcacgtg ccgacggggt tagctgacgg
      601 gctgggagtt gaaagtctcc ctcaggtcca acgcacacat cccggacctg atatgcccct
      661 gccgttcgcg tacacacacc agtcgcgaac gacgaagcgt ttgctgattt cgttccacac
      721 gacacaaatc gtgaaactcg tcacagacgc tttggtttcc ccgcgcccat ccggatgaac
      781 ggataggctt aggcggtcaa gcacacgcga gtcaataggt cgagcacggt gtgtcaaccc
      841 acaaacttgc ccaatgtgca aactttttca gcggggtgcc gtggaaaaac gcgggttttg
      901 aatgcttgct cagacatctt ccgatggtga tcggaaaacg cgcggaagct aggcagaata
      961 tgcggtttgc gacggtggcc atcacgccgg gttcggaggt gctccgacga agcgaatttt
     1021 tccgctcgcg cgccgcagaa tccgggtcgt cggagtaact ccgaacccgg cgtgggggcg
     1081 tcggtgtatc cagcgcgatc atccgcatat gatgccgccc ctcgatggca gcactgactt
     1141 tcgaaatcac gacgaccgat ggatcgaccg gcgctcgaac gggtcgcgtg acgacgcggc
     1201 atgggcgctt tgacacgccg gcgtttatgc cggtggggac gcaggggtcg atcaaggggc
     1261 tgctgccgga tcatgtggcg gcgaccgggg ctcaatgcat tctggccaac acctatcacc
     1321 tgatgcttcg gccgggggaa cgcgtcgtgg ctcagctggg cgacctgcac gcgttcatgg
     1381 gttggccggg gccgatcttg accgacagcg gggggtttca ggttttctca ctgtcggaca
     1441 tcaacaagat ttccgacgat ggcgtgacgt ttaagagtca cgtcgatggg tcgatggtgc
     1501 acctaacgcc gcggcggtcg atggaggtgc agaatgcgct gggcgcggac atcatgatgg
     1561 cgttcgacga atgcccaccg gcggatgcgc ctgttgaata tcacgaagag gcggtcgcgc
     1621 gcacgctgcg atgggcggcg gagtgtgtgg aggctcacgc tcggccggat gaccaatcgt
     1681 tgttcggcat cgttcagggc ggcgtgcacg aacagctgcg cgcattgtgc gcggagaaac
     1741 tcgtcgagat ggacctgccg ggttacgcgg tggggggact agccgtgggg gagggattcg
     1801 aggcgatgaa ggcgattctc ggcttcacca cgccgctgtt gccggtgaac aagccgcgct
     1861 acttgatggg cgtcggtttt ccgcgcgaca tcgtggcggc ggtggcgcgg gggatcgaca
     1921 tgttcgattg cgtgatgccg acgcgcaacg ggcggaacgc gtacgcgttc acgatggacg
     1981 gggcgattcg cctgcggaat agccgattta ctgaagataa aggtccgatc gagaccggct
     2041 gtgactgcta cgcctgccgg acatttacgc ggggggcgat ccgccacttt tttttcgcgg
     2101 gtgagatgct tggcccgatc ctgttgtcac tgcataatat ccgattctac caacggctga
     2161 tggcggacat ccgagggtcg atccgtgaag gacggtttga gtcgttctat cgagatgacc
     2221 cgcgcagcag gatgggaccg agtgaggttg cgtgattacg tgaatacgtg attacgtgac
     2281 aaaggcaaga gcaaggtaat cgatcacgta atcacgtaat cacgtaatca cgcaaggaga
     2341 aggtttgaac agcatcatcg cgtttctcgc ccaggcggcc cccgcggccc cgacgtcgca
     2401 gccggtgccg ggttgggctc ggttcatcac gcagtccggt ccgctgttgc cgttggcgat
     2461 cgggttcatc ttcctgatga tgttcatgaa ccggtcgaag aaaaagcagg aacacagtcg
     2521 cgacgaaatg ctcaagcaac tgaagcgtgg cgatcgtatt actaccatcg gcgggatcca
     2581 cggcaccgtg ttgcggaccg aggactcgcg cgtcgaagtg aaggtggacg aaggcaacaa
     2641 caccaagatc tggttctccc gcagcgcgat ccacaaggtc gccgaagagg agaaggcggt
     2701 gtagcggcga cgcctgcgtc gcccattggt caacatcgca aaaggcgacg caggcgtcgc
     2761 agctacacat tcctgcattt cgtatcatgg aacaaaacca aaccggtcgt gttattttta
     2821 ttcttgccgt cttgttggcg gcggtgctct ccattgtgcc gcagccgctg aagctgtttc
     2881 ggcatgatgt gccgctgggc gagaagctga acctcaagcc cggcatcgat atggtcggcg
     2941 ggaccagcct gctgtaccag atcaagaccc ccgaaggcgg gttccacggc aacaacggcc
     3001 agacgttggc cgaagcggtg atggaatcgc tgaagcgccg cgtggacccc aacggtgtgc
     3061 gcaacctgat ctggcgcccg caggggaccg atcggctgga gatccagttg ccggcttcgc
     3121 cgaattctga agaagcgaag aaggcgcgcg aggcgtacgt cgcggcccag cagcagctga
     3181 gtgatacgaa cgtctcttcg acagaagtga tcaatgccgt cgagcagctc aagggggatg
     3241 agcgccgcga tcgcctgaac cagctggcgg cgggctcgga cgctcgagcg aaactgttcg
     3301 gcgcgctggc atcggcgtac gaccagaagc aggcggccca cgagaagcgc gatgctgagc
     3361 ttgaggcgca gaagactgag gaatacgaca agctcaagtc gcaggtcgag cagagcaacc
     3421 tggccgtggg ccagctggaa gcgatgctgg acgccccggc ggaccagcgc acggcgaagc
     3481 tggcggagct gaagaagcag tatgccgact atccggcgcg cgtctcggcc atcgacaact
     3541 tcgtcaagtc gtacgatgag tacaacaagc tcaagggctc gatcgacgac gccgcccagc
     3601 tcaagcgaga gctgcaaggc tcgggcgtgc tcgagttcca catcctggcc aacgacctcc
     3661 cgcgcgccga atacatcgcg atggtcgatc ggctcaagag caagggcccg cgcgtcgagg
     3721 cgggcgatgt cgtcaagtgg ttcatggtcg atcgcccgca ggacgtgcaa tccttcggca
     3781 acgaagtgca agagtggaac ggcaaatatt acgcgctgtg ctacatcacc gacgacaagt
     3841 cgatggtgaa cggtccgcat acgaaccatt ggacgctgga gtcggctcat ccgtcatccg
     3901 acggtgaagg cggtcgcgcc gtcggcttta ccttcgatgc cgtcggtggc gattacttca
     3961 gcacctttac gcagaataat ctgaagaagc cgctggcggc gatcctcgac aacaaaatcg
     4021 tctcggttgc caacatcaat tcgcgcattg gccgcgaagg tgtcatcacg cgcggcaacg
     4081 gttacgacga cgaggaattc aactacctga tcagcacgtt gaacgccggc agcttgccgg
     4141 cgacgcttgc cgatgaaccg atcagcgaac acacggtcgg cccgcagctg ggtgaagaca
     4201 acctccgccg cgggttgatg gcctgcgggt tcgggctcat catcgtggcg gtcttcctcg
     4261 tcggttatta ctacttggcc ggtgtggtcg cgacgttcgc cgtcttcatg aacgtcatct
     4321 tgatcctggg catcttggct gcgttcaacg cgacgttcac gctgccgggc attgccggca
     4381 tcgtgctgac gatcggtgcc gccgtggacg ccaacgtgct gatcttcgag cgcctgcgcg
     4441 aagagcagca tcacggcctc agcatccgca tggcgatgcg caacgcctac ctgcacgcgc
     4501 ggggcgccat ctgggactcc aacgcgacga cgatcatcac cagtgcgatc ctgatctggc
     4561 tcggcagcga agaagtgaag ggctttggcc tgacgctgct gatcggtttg gccagcagct
     4621 tgttcacgtc gctgttcgtg acgcggacga tcttcaacat catgatcgac aagttcggtt
     4681 accagcattt tggctcgcta ccgctgacgt tccccaagtg ggaccagttc ctcaagccga
     4741 atatcgactg gatgaagatc gtgccgtatt gcctgacgtt cagcgcggtc ttcatcgcgc
     4801 tggggatgac ggcgttcgtc atgaaggccc gcgcgcacga gctggccgac atcgacttcg
     4861 ccagcggcac gcaagtgcag ttcgaattga aggaccggac caagatcgaa gacctgcgct
     4921 cgatgttcac caaggcggac aacccggcgc tgccgtcgcc gagcctggtg tcggtgaatc
     4981 acgacgacct cacgtacgag atcgtcacgc cgaatgtcga tgccaaggcg gtgcgcgaag
     5041 cggtgctgag cgtcgtcggc accaagatca agaccgacct gccgagcaag ttcgatcacg
     5101 tggacgagtc gatggaccag gcgatgggct cggccatcca gccgatcgtc aagacgccgc
     5161 tggtcatcaa cggcttcacc gtcccgcgcg ccgagaacta cctcggcggg gcggcgatcg
     5221 tgctgaacca cctgaacccg ccgttgaagg tgagcgagat caagacgcgc atcgaccaga
     5281 tgcgcgtgca ggacgagtcc gcgcagagcg gcgcggaggg cagccgctat cacgacttca
     5341 cggtcgtttc acccaacggg cctgaccaac cgaccagcac cgccatcgtg ctgacaggcg
     5401 acccgaacct gccgtacgac aaggatgaag gcaagtggcg cgaagagttg gtggcgccga
     5461 tgtggaagct gatcaaccaa tcgatcaacc gcccggcgac gctgcagaag gtgaacaact
     5521 tcgacgccgc cgtcgccggt gacacgcaga aggccgcgtt ttattcgctg gtgctctcgt
     5581 cactggtcat catgttttac atctggttcc gattcgggaa cctcaaatac ggcaccgcga
     5641 cggtgctggc gatgatccac gatacgttcc tggtggtggg ggcgatcggg ctgtcgcact
     5701 ggatggtgca gtacacaccc gggctggcgc ggatactgtt ggtcgaaccg tttcggatca
     5761 acctgactgt ggtggccgcg gtgttgacgg tgatgagtta ttcgatgatc gatacgatcg
     5821 tggtgttcga ccgcatccgc gagaaccgcg ggaagttcgg gcacatgagc cggaagatca
     5881 tcaatgactc gattaaccag acgctgagcc gaacgctgtt gacgtgcggc atcacgatca
     5941 tgacggtcgc cagcatgtat tttgtgggcg gtcccggcgt gcacggcttt actttcgtgt
     6001 tgctcgttgg catattggtt ggcacgtatt cgtcaattgc tgtagcggcc ccaattttgt
     6061 tgatgggtca cgaagcgaag ggcgacagcg gtggatcggg tggcgcggga tgggcagctg
     6121 caaagaaccc cgcaccgaca ccgggcaaaa ccgaacgggc tgtggctgtt gctgtaaaca
     6181 aagtgaagac ggccggctga attcagtgac tgtgtgagcg attgagttgt acgtcgcacg
     6241 cctggctgga ttgcagcgcg tgcacggggg aaaaccaaat cgttcactgg gaaggagtcc
     6301 cacagatgcg caaggatgcg aagatcgggt tcgccattgg tggcgtcctg ctggccgttc
     6361 tcaccgttta cgcgatcgtt gttcccaagc acaaccgaac gccgaatgcc gccggtgggg
     6421 tgacgctggt cacgccgacg acggcggcgc cgtcaattca atcgccggct acgacgggga
     6481 acgatcttcc gcccgcggtg gctggggcgc tcaacggcga caggcgcgcc gatcccaagc
     6541 ctgaaccggc cgttgcaacc aacgacacga acaagagcag cgatggcgtg aattgggcga
     6601 agctgcttag cggcagccct gatgcgccga cgctaatgag caccacaccg tcgtccagcg
     6661 acgacgcgac gcccgcgccg gccgcttcgg cgaaagagac gcaggtggcc aaggccacgc
     6721 cgactccgtc ggctgatgcg gcttccgatc ccgcgcccga gacgacgccg ttgatggcca
     6781 aggcgaacat ctcgactcct gcaacgcagc cgctggcgac tggtcgcacg tacacgatta
     6841 agcccggcca gacgctgtcg agtgtcgccg ccgaggtcta cggcaaccag cgcttctacg
     6901 tcgcgatcct tcgcgccaat cccacggtca accccgctcg tctgcgtccg ggcacgaagg
     6961 tcacgctgcc ggacatctcc gacgtccagc ccaaggcgga tgccccggtg ctggccgcca
     7021 ccgcgaagca aacccagagc gagtcgaccg agaaggccag cagcggccac acgtacaaag
     7081 tcgaatccgg cgacaacctc tatcgcatcg cccgcaagct ctacggctcg cctaagctgg
     7141 ccgagacgat ctacgaattg aacaaggacc gcatcggccc cgacaagacg aagctgaaac
     7201 taggcatggt cctggacctg ccgcagtcgc ccagcgccag cgcgaccgcg tcgtcgcgct
     7261 aagcgcaaag cgtcacagcc cgataaatcc tgtgcaatcg cgcgacagcc cccgcgccgg
     7321 gttcggaggt acgccggcga cccggattat tcttgctcca cgcgcggaag aatccgggtc
     7381 gtcggcgtac ctccgaaccc ggcgtggg
//
LOCUS       DATIMH010000920         1976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_218758, whole genome shotgun sequence.
ACCESSION   DATIMH010000920 DATIMH010000000
VERSION     DATIMH010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218758"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VLI90_16015"
     CDS             complement(<1..935)
                     /locus_tag="VLI90_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSV15768.1"
                     /translation="MAEPTNIKANILVVDDEQEHAQVMCEALTRLGHKCDVTYNLAEA
                     QAKLNRKNYDVIVTDLMMDGRRDGLEVLRRAKALTPPPPVLLVTAINDVPTSKQAMHE
                     GAYDYITKPLDLEDFRNQVNRAAERAALLKQNQALQEQLLDTAGFEGIIGTSAVMQRV
                     VQTARQVAASDIPVLIMGESGTGKELIARAIHNNSRRRKQRLVALNCAGLSESILEDE
                     LFGHVRGAFTGATTDREGRFEHADGGTLFMDEIGDMPGPMQAKLLRVLENGEVVRVGS
                     NDPLQVDVRLISATNRKLEEMVAERQFREDLYFRI"
     gene            complement(1073..1867)
                     /locus_tag="VLI90_16020"
     CDS             complement(1073..1867)
                     /locus_tag="VLI90_16020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV15769.1"
                     /translation="MAFAWGLIVGVIVCALVGAAIGYAAYQRMERLQRRTREAERLAE
                     LGTLTGGLAHEIKNPLSTVQLNLQLLEEDLDPKDPTQQRVINRLHTVQREAGRLRETL
                     DDFLRYAGKIELDKRATDVNELLEELVDFFSPQALLNRVQLRLRKFDGQLIVPLDARL
                     IKQAVLNLMINATQAMTTGGGELILSASKDDREACIDIIDTGGGIPPDALANVFTAYY
                     STKKGGTGLGLAISQRIAREHGGSLTVKSEVGKGSDFTLHLPLGMN"
BASE COUNT          332 a          617 c          602 g          425 t
ORIGIN      
        1 ttgatgcgga aatacaggtc ttcgcgaaac tgcctttccg cgaccatctc ttccagcttg
       61 cggttggtcg cgctgatcag tcgcacgtcg acttgaagcg gatcattact gcccacgcgg
      121 acgacttcgc cgttttcgag cacgcgcagc agcttagcct gcatggggcc gggcatgtcg
      181 ccgatttcgt ccatgaacag cgtgccgccg tcggcatgtt cgaagcggcc ttcgcggtca
      241 gtcgtcgcgc cggtgaacgc cccgcggacg tggccgaaca gttcgtcttc aagaatgctt
      301 tcgctcaacc ccgcgcagtt gagcgcgacc agccgctgtt ttcgccgccg gctgttgttg
      361 tggattgcgc gggcgatcag ctccttgccg gtgccgcttt cgcccatgat gaggacgggg
      421 atgtcgctgg ccgccacttg ccgggcggtc tgcacgacgc gctgcatcac tgcgctggtg
      481 ccgatgatgc cttcaaaccc cgcggtgtcg agcagctgtt cctgcagcgc ctggttctgc
      541 ttcaggagcg ccgcccgctc ggccgcccga ttcacctggt tgcgaaaatc ttccaggtcc
      601 agcggcttag taatgtagtc gtacgcccct tcatgcatcg cctgtttgct ggtcggcaca
      661 tcattgatgg cggtgacgag caacaccggc ggcggcggcg tgagcgcctt agctcgccgc
      721 aagacttcca gtccatcgcg ccggccgtcc atcatcaggt cggtgacgat cacgtcatag
      781 ttcttgcgat tcagcttcgc ctgcgcctcg gccaggttgt aggtgacatc gcacttgtgc
      841 ccgagacgcg tcagcgcctc gcacatcacc tgcgcgtgct cctgctcgtc gtcgacgacg
      901 aggatgttgg ccttgatgtt tgtaggttcc gccatatcag ctaacagata ttaaccacga
      961 aggcacgaag agcacgaagc cggcacacac tcaatttcat ttcgatccgg cttcatttgt
     1021 gtgcatctct ggtaaattct cttctcttcg tgcccttcgt gcccttcgtg gttcaattca
     1081 ttcccagcgg caaatgcagc gtgaagtcag atcctttccc gacttcgctt ttgaccgtca
     1141 gcgagccacc gtgctcgcgc gcgatgcgtt ggctgatcgc aagacccaat cccgtgccgc
     1201 ccttcttggt ggaatagtac gccgtgaaga cattcgccag cgcgtcgggt ggaatgccgc
     1261 cgccggtgtc gatgatgtca atgcacgctt cgcggtcgtc cttgctcgcg gacaggatca
     1321 attcaccccc gccggtcgtc atcgcctgcg tggcgttgat catcaggttc agcaccgcct
     1381 gcttgatgag ccgggcgtcg agcggcacga tgagttggcc atcgaacttg cgcagccgca
     1441 gctgcacgcg gttgagcagc gcctgcgggc tgaagaaatc gacgagttct tcgagcaatt
     1501 cgttcacgtc ggtcgcccgc ttgtccagct cgatcttgcc cgcgtatcgc aggaaatcat
     1561 ccagcgtctc gcgcaaccgc cccgcttcgc gctgcacggt gtgcagacgg ttgatcacgc
     1621 gctgctgggt cggatctttg ggatcgaggt cttcttcgag cagctgcagg ttcagttgaa
     1681 cggtggagag gggattctta atttcgtgag cgagcccgcc ggtgagggtg ccaagctcag
     1741 ccaatcgctc cgcttcgcgc gtgcggcgct gaaggcgttc catgcgctgg tacgcggcgt
     1801 aaccgatcgc cgcgccgacc agcgcgcaga cgatgacgcc aacgatgagt ccccaagcga
     1861 acgccatggg cggggaaggt acgggcgggg ataagcgaag gaaagcgcgg cgacagtatc
     1921 agcgcagcgg tgaccgattt cgaagaggcg acggaattgc gatcagcgcg cgcccg
//
LOCUS       DATIMH010000921         1884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3108490, whole genome shotgun sequence.
ACCESSION   DATIMH010000921 DATIMH010000000
VERSION     DATIMH010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3108490"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1807)
                     /locus_tag="VLI90_16025"
     CDS             complement(131..1807)
                     /locus_tag="VLI90_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15770.1"
                     /translation="MNFSSTCIALGLLATAFTVCRAASAAEYQWSCPIAPATTQPSET
                     PRAYLWIPPDCRQVRAVVVGQHNMLEEDILEHPEFRAAMAKLGIAEIWITPALDGPFD
                     PAKSGERFDAMLHALADISGYGELAFAPIAPIGHSAAASYPWNFAAWAPQRTLCALSI
                     HGDAPQTPLAGFGRPNASWGDRNIDGVPGLMVMAEYEWIDARLAPATVYHVAHPKAPI
                     AMLAEPGDGHFDACDDLVRYLVMFLSKCVEQRLPALAETPLDRPPVLKAIDPSAGWYV
                     QRWTPVVGRTIPPGPVQRYGGDGVDAFWAFDEEMAQATQNYHADQIGKRPQLIGFVQN
                     GQLVPATRTHEMVHLKFLPEADGETFKLQTRFLDTVTSMDPQNDGAGKNNHVRWTGLP
                     AGSPLGHASGGGPIQLHRIEGPIEQIDADTFRLSFYRGCTSRQAIWVYATHPGDEHYK
                     SAVQQASMPVPHNTGGDEQTITFPAIADQQTSATTVTLAATSSAGLPVHYYIREGPAE
                     VRGDALKLLPIPPRAKYPIAITVVAWQNGRSGESRVKTAQMVERTFQITR"
BASE COUNT          312 a          629 c          601 g          342 t
ORIGIN      
        1 atccgtgaca cgggcctccg gcctgtgcgc acaagcgatc cgattgaagg aagtcgtgag
       61 ccatctactt ccgcaccgct cgcgcgggcc ggaggcccgt gtcacggtgg cgcccgttca
      121 tccacgcgat tcaccgggtg atctgaaacg ttcgctccac catttgcgcc gttttcacgc
      181 gcgactctcc agagcggccg ttctgccagg cgaccaccgt gatggcgatc ggatatttcg
      241 cccgcggtgg gattggcagc agcttcagcg cgtcgccgcg aacttccgcc gggccttcgc
      301 ggatgtagta atgcaccggc aggccagcgc tcgatgtcgc ggcgagcgtc accgtggtgg
      361 cgctggtctg ctgatccgca atcgccggga acgtaatcgt ctgctcatca ccgcccgtgt
      421 tatgcggcac cggcatgctc gcctgctgca ccgccgattt gtaatgctcg tcaccgggat
      481 gggtcgcata gacccagatc gcctgccgcg atgtgcagcc gcgatagaag ctcaggcgaa
      541 atgtgtcggc gtcgatttgc tcgatcgggc cttcgatgcg atgcagctga atcgggccgc
      601 caccgctggc gtgaccgagc gggctgcccg cgggcaagcc ggtccagcgc acgtgattgt
      661 tttttcccgc cccgtcgttc tgcgggtcca tgctggtgac ggtgtcgagg aagcgcgtct
      721 ggagcttgaa cgtttcgccg tccgcttccg gaagaaactt aagatgcacc atctcgtgcg
      781 tgcgcgtggc cggcaccagc tggccattct gcacgaagcc gatcaactgc ggccgcttgc
      841 cgatctggtc ggcgtgatag ttctgcgtcg cctgcgccat ctcttcgtcg aacgcccaga
      901 acgcatccac cccatcgccg ccatatcgct gcaccggccc cggtggaatt gtgcggccca
      961 ccaccggcgt ccagcgctgc acgtaccagc cggcgctcgg gtcgatcgcc ttcaacaccg
     1021 gcggtcgatc gagcggcgtt tccgccagcg ccggcaggcg ctgctcgacg cacttgctca
     1081 ggaacatcac cagataacga accagatcat cgcacgcgtc gaagtgccca tcgccgggct
     1141 cggccagcat cgcgatcggc gctttcggat gcgcgacgtg atacaccgtc gccggcgcca
     1201 gccgggcgtc gatccactcg tactcggcca tcaccatcag gcccggcaca ccatcgatgt
     1261 tgcgatcacc ccagctggcg ttcggccggc cgaagcccgc gagcggtgtc tgcggggcat
     1321 cgccatggat cgacagcgca cacaacgttc gctgcggcgc ccacgcggca aagttccacg
     1381 gataactggc ggcggcggag tgaccgatcg gcgcgatcgg cgcgaacgcc aactcgccgt
     1441 agcccgaaat gtccgccagc gcgtgcaaca tcgcgtcgaa tcgttcaccc gacttggcgg
     1501 gatcgaacgg cccgtcaagc gcgggcgtaa tccagatctc ggcgatgccg agcttcgcca
     1561 tggcggctcg gaactccggg tgttcgagga tatcctcttc cagcatgttg tgctgcccga
     1621 cgacgaccgc gcgaacctgc cggcagtcgg gtgggatcca caagtatgcg cgaggcgttt
     1681 cgcttggctg cgtcgtcgcc ggcgcgatgg ggcaggacca ttgatactcc gccgcgctgg
     1741 ccgcgcgaca aacagtaaac gcggttgcga ggaggccgag cgcgatgcag gtggaggaga
     1801 agttcatttc agttttttga ggaagactta gctctgcgcc gcagaatccg ggtcgtcggg
     1861 agtaccctcc gaacccggcg tggt
//
LOCUS       DATIMH010000922         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4262535, whole genome shotgun sequence.
ACCESSION   DATIMH010000922 DATIMH010000000
VERSION     DATIMH010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4262535"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1604)
                     /locus_tag="VLI90_16030"
     CDS             complement(276..1604)
                     /locus_tag="VLI90_16030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV15771.1"
                     /translation="MHTSHRLTPIQWLICIIAAIGFAFDTYELLMFPLINRPALTELG
                     HLEFGSAEYRRWLGLLFYVPAVCGGVFGLLGGYLTDRLGRRRVLTWSILLYALSAFAA
                     GYTTSLPSLLILRCATFIGVCVEFVAATAWLAELFHDPGQRERVLGWTQAFGSIGGVL
                     VTGVYSLIVSHTASLPAIHGVHEPWRYTLMSGVIPAIPLICIRPFLPESPLWREKKAA
                     GTLRRPSVADLFAPQFRRTTIVTTIMFACSFGAAFGAIQQMPQQIAPGLSDVRTLPPA
                     ARQQAVAAVQWFQEMGGLAGRILLAFLAVRVLSRRSLLWIFQIPGLLILPVVFLVAPH
                     EPLAWAKWGIFAVGVCTVAQFSFWGNYIPKVFPTYLRGTGESFAANVGGRMIGTAAAL
                     VTTALAGSMPGGSPSTQMAYAAALVGLLVYAVGFAASFFLPEPGRDGLLE"
     gene            1652..2794
                     /locus_tag="VLI90_16035"
     CDS             1652..2794
                     /locus_tag="VLI90_16035"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HSV15772.1"
                     /translation="MVPFDFEPRTRVVFGRGTVERVGQLAKGFHFQRTLLVADRGLVK
                     AGHVAAVTAALESDNVAVVPFHDFGENPDSQMIESGRRFAEPHRVDSIVALGGGSSLD
                     CAKGINFVLTNGGAIGDFRGYGKASKPLLPMIGIPTTAGTGSEAQSYAVISDASTHIK
                     MACGDPSAAMRVAILDPELTMTAPHHVTAMAGFDAIAHAVETAVTVRRTPLSDMFSYR
                     AWHLLNTAFERVLLHPADIEARSSMQIGAHFAGMAIEQSMLGAAHACANPLTARYNLS
                     HGLALAILLPHVVRWNADVARERYAMFMGSPRRRARDEDAAETLALRLEDLARAGGLS
                     VKLSDSDVEESAISELAAQAAAQWTGTFNPRPLDAEGAAEIYRAAF"
BASE COUNT          569 a          973 c          923 g          484 t
ORIGIN      
        1 agcacgccgc cgcagcgcgc agccggaaag cgcgcgcggc gattcggtgc ggcgacttcg
       61 tgctgtgtga tccgcgtaga ccccaacggc ccgcccggcg catcagtgcg cctgacatgt
      121 gtcgtccacc ggcatcccta gtcgccgcgt gaccgccagc gtacgagccg cgcgcgattt
      181 cccggtgtgc ggagcggcgg cggacgccgg ccccgctcgt ccgcggcgtc ctccaccgat
      241 ccggcgaaac ggttctcacg ctcgggatct cgctcctact ccagcaaccc atcacggcca
      301 ggttcgggca gaaagaaact ggcagcgaag ccgaccgcgt aaacgagcag accgaccagc
      361 gccgcggcgt atgccatctg cgtgctcggg cttccacccg gcatgctccc cgccagcgcc
      421 gtcgtcacca gcgccgcggc tgtgccaatc atgcggccgc cgacattcgc cgcgaagctc
      481 tcgccggttc ctcgcaggta ggtgggaaat accttcggaa tgtagttacc ccagaaactg
      541 aattgcgcga cggtgcaaac gccgaccgcg aagatccccc acttcgccca cgcgagcggc
      601 tcgtgcggag cgacgaggaa taccactggc aggataagca gcccgggtat ttgaaagatc
      661 cataggagcg agcgccggct caaaactcga accgcaagaa atgcgagcag aatgcggccg
      721 gccagcccac ccatctcctg aaaccactgc accgcggcga cggcctgctg gcgcgcggcg
      781 ggcggcaacg tgcggacatc tgacagtccc ggcgcaatct gctgcggcat ctgctgaatg
      841 gcgccaaagg cagcgccgaa gctacacgca aacatgatcg tggtgacgat ggtcgttcgc
      901 cggaattgcg gcgcaaaaag atcagccacg ctgggacgcc gcagagttcc cgcggctttc
      961 ttttcacgcc acagcggaga ctcaggcaga aagggacgga tgcaaatcaa cgggatcgcc
     1021 gggatcaccc ccgacatcag cgtgtagcgc cacggttcat ggacgccatg aatcgcaggc
     1081 agcgacgcgg tgtggctcac gatcagcgag tacacgccgg tcacgaggac accgccgatc
     1141 gagccaaagg cctgcgtcca tccgagtact ctctcgcgct gcccgggatc gtggaagagt
     1201 tcagcaagcc atgcggtcgc ggccacgaac tcgacacaga cgccgatgaa cgtggcgcac
     1261 ctcaggatga gaagagacgg gaggctcgtg gtgtaaccgg ccgcgaatgc cgagagcgcg
     1321 tagagcaaga tgctccacgt caatacgcgc cgccgcccaa gccggtccgt caggtaccca
     1381 ccaagcaagc cgaacacacc accacagacc gccggcacgt aaaacaacaa cccgagccat
     1441 cgccggtact ctgcactgcc aaactcgaga tggccgagtt cggtcaacgc cggacggttg
     1501 atgagcggga acatcaacag ctcgtaggtg tcgaaggcga aaccgatcgc ggcgatgatg
     1561 cagatgagcc attggatagg cgtaagccgg tgcgatgtgt gcatatcgag agcgtcggag
     1621 attaattcgg gaatggtaga gtctactgca gatggttccc ttcgactttg agccacggac
     1681 gcgtgtcgtc ttcgggcgcg ggactgtcga gcgggtaggc caactcgcca aagggtttca
     1741 ttttcagcgc acgctgctcg ttgccgatcg cggactcgtc aaggctggcc acgtggccgc
     1801 ggtgaccgcc gcgctcgaga gcgacaacgt cgccgtcgtg ccgtttcacg actttggtga
     1861 aaaccccgac agccagatga tcgaatcagg gcgccggttc gccgaaccgc accgcgtcga
     1921 ttccatcgtg gcgctcggcg gcggcagttc gctggactgt gcgaagggca tcaacttcgt
     1981 tctgaccaac ggcggcgcaa tcggtgactt tcgcggctac ggcaaagcat cgaagccgct
     2041 gttgccgatg attggtatcc caacgaccgc cggcacgggg agcgaagcac agagctacgc
     2101 cgtcatctcg gatgcatcca ctcacatcaa aatggcgtgc ggcgatccgt cagcggcgat
     2161 gcgcgtcgcg atcctcgatc ccgaattgac gatgaccgcg ccccaccatg tgacggccat
     2221 ggccggcttc gatgccatcg cgcatgccgt cgaaacagcg gtcacggtgc ggaggacacc
     2281 gttgtcggac atgttctcct accgtgcctg gcacctgttg aacaccgcct tcgaacgcgt
     2341 gctgctgcat ccggcagaca tcgaggcgcg ctcgtcgatg caaatcggtg cgcatttcgc
     2401 gggcatggcc atcgagcagt cgatgcttgg agcggcgcac gcgtgtgcga atccgctgac
     2461 ggctcgctat aacctgtcgc acgggctggc gctcgccatc ctgctgccgc atgtcgtccg
     2521 ctggaatgcc gacgtcgcgc gcgagcgata tgccatgttc atgggatcgc cacgccgtcg
     2581 cgcgcgcgat gaggatgccg cggagacgct ggcactccgg ctcgaggatc tcgcacgcgc
     2641 cggtggtctg tcggtcaaac tgtccgacag cgatgttgaa gagagcgcga tttctgaact
     2701 cgccgctcaa gccgcggcac agtggaccgg cacgttcaat ccacgaccac tcgacgccga
     2761 aggcgctgcc gaaatctacc gcgctgcttt ctaatccgag gggcttcgcc cctcggactc
     2821 cccgacacgc tctctcgcgc gccgcttcgc cggcccgctc cgttcgcgtg gctcactcgc
     2881 tccgctcgtt cgcccggatc gcttcgacgg tccgggcttt tttcgtggag tttggctgtt
     2941 gaggcgcgt
//
LOCUS       DATIMH010000923         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_800121, whole genome shotgun sequence.
ACCESSION   DATIMH010000923 DATIMH010000000
VERSION     DATIMH010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800121"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VLI90_16040"
     CDS             complement(<1..606)
                     /locus_tag="VLI90_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15773.1"
                     /translation="MPATAVERLECRMMLTGDFGFVLNRGAEFADSASAVASDSKGDV
                     FITGSFEGTVDFDPFGPKATHASLSTDSEGLFAAKYDRDSHLLWVVKIYDNDGVRTSG
                     PVARDIAVDTAGNAIVTYIVTSGPPVSNATIVKISPSGLTLWSRTIIGANVVGVATTP
                     SNEIVTLGTLGAGATDVDPGAGTFTLTPPDGEDAFVEKLGPG"
     gene            895..1548
                     /locus_tag="VLI90_16045"
     CDS             895..1548
                     /locus_tag="VLI90_16045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HSV15774.1"
                     /translation="MAKTKEQYVNQILDAGVVAVIRAKSKDQLMDIAKALLDGGVPAI
                     EVTMSTPKAIAGIEMLADAMGDKAVVGVGTVIDAATCRDAIAAGAQFVVSPVFDPEIV
                     ATTKRYGKISIPGSFTPTEILRAWSAGADVVKVFPSTTLGPGYFKDILAPLPQLKLTP
                     TGGVDLKNTGEWIKAGAACIGVGSALVSKDALAKNDWAAITNSAKAFVDAVKKARNG"
     gene            complement(1752..>3911)
                     /locus_tag="VLI90_16050"
     CDS             complement(1752..>3911)
                     /locus_tag="VLI90_16050"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HSV15775.1"
                     /translation="AVVAGCIDEDKEQAMARTRERKIAIATRIRDLAVGRFGLRDEDL
                     MFDPLVLPISTGIEEDRRNALETIEGTRRISQELPRCHTVLGLSNVSFGLKPAARVVL
                     NSAFLHELREAGLTGAIVHASKILPKNRIPQEQWDAALDLIYDRRREGFDPLTHFISL
                     FPDDAGGPTDAKPQAADAPVEEKLRRHIIDGEKRNLIADLDEARQKYPPLAIINDHLL
                     DGMKVVGDLFGSGQMQLPFVLQSAETMKAAVAYLEPFMEKSEGSTKGKIVLATVKGDV
                     HDIGKNLVDIILTNNGYTVYNLGIKQPVSDILKEAKAKSADAIGMSGLLVKSVGVMKE
                     NLEEMNAVGIDVPVLLGGAALTKDYAVNELAALYKGKLFYCKDAFAGLNTMDAVVGGH
                     LEDAFSKQQADGARRAELRATKGYKGEDVSTLPAKSDVKTDNPVPTPPFWGRREVKDI
                     PLEQVFAYINPNALFLGQWDFKKKGLSDEAYERLLDDKARPVYAELQRRAIAEKLLTP
                     KVVYGYFPVQSEGDDIVVYHTEEFAGCACGQHGASAGKLQPAGAPRERMRFTFPRQNT
                     RRRLCVADFFRSKESGQYDVLGVQLVTVGDRAAELAQELFKANKYQDYLYLHGFGVES
                     AEGLAELWHKRMRQELGFGSEDAPKIAQLFQQGYRGSRFSFGYGACPNLEDRAKIVEL
                     LKPDSIGVSLSENFMLVPEQSTDALVVHHPQAKYFDV"
BASE COUNT          696 a         1263 c         1198 g          754 t
ORIGIN      
        1 accgggaccg agcttttcga cgaacgcatc ttcaccatcg ggcggcgtca gcgtgaaggt
       61 gccggcgccg gggtccacat cggtcgcgcc tgcgcccagc gtgccgagcg tgacgatctc
      121 gttggacggc gtcgtcgcaa cgccgacgac gttggcgccg atgatggtgc gcgaccagag
      181 cgtgaggccg ctcggcgaga tcttcacgat cgtcgcgttg ctcaccggcg gcccggacgt
      241 gacgatgtac gtgacgatgg cattgccggc ggtgtcaacg gcgatatcgc gcgcgacagg
      301 gccggacgtg cggacgccgt cattgtcgta gatcttcacc acccaaagca ggtgcgaatc
      361 gcggtcgtac ttggcggcga acagtccctc tgaatccgtc gaaagcgacg cgtgcgtggc
      421 cttggggccg aacgggtcga agtccaccgt tccctcaaat gatccggtga taaacacgtc
      481 gccctttgaa tcgctggcga cggcggatgc gctgtcggcg aactcggcgc cgcggttcag
      541 cacgaatccg aagtcaccgg tcaacatcat tcgacattcg agccgttcga cggcagtggc
      601 gggcatggtt tctcctattt gctgagaggg ctcgctggta aggcgcgtcg gtgacttgtc
      661 ccccgctccc agtacgccgg gagagggtcg gggtgagggt gatttgggtt cgaaacccgt
      721 tcgaccttcg aaatgaccct caccctaacc ctctcccggc gtaccgggag agggaacaag
      781 attgccgcgc gcctcggact tagcgcgctc gtgtacccaa acagcataac cttgccatcc
      841 cgcaatcgac acagaattcc cactggtccg tttccgtgat aaaacacgcg aatcatggca
      901 aaaaccaaag agcagtacgt caatcagatc ctggacgccg gcgtggtggc ggtcatccgg
      961 gccaagagca aggaccaatt gatggacatc gccaaggccc tgctagacgg cggtgtgccg
     1021 gccatcgagg tgacgatgtc cacgcccaag gcgatcgccg gcatcgagat gctggccgac
     1081 gcgatgggcg acaaagcggt ggtcggcgtg ggcacggtca ttgacgcggc gacctgccgc
     1141 gatgcgattg ccgccggcgc gcagtttgtc gtctcgccgg tgttcgatcc ggagatcgtg
     1201 gcgacgacca agcgctacgg gaagatcagc atcccgggat cgttcacgcc aacagaaatc
     1261 ctgcgggcgt ggtcggccgg cgcggatgtg gtgaaggtct tcccgtcaac gacgctcggt
     1321 cccggatact tcaaagatat tctcgcgccg ctgccgcagc tgaagctaac gccaacgggc
     1381 ggggtcgatt tgaaaaacac cggcgagtgg atcaaagcgg gcgccgcgtg catcggcgtc
     1441 ggttcggcgc tggtttcgaa agacgcgctg gcgaagaacg actgggccgc catcaccaac
     1501 agcgccaagg ccttcgtgga cgcggtgaag aaggcgcgaa atggctaatg aattgccgct
     1561 tgcggcttag cgtctgttta ccgatcgctt gccagacacc agcgcgcggc ggaattttct
     1621 atcgggtcgc ctcgcccacg tcggtaccgg cacggtcacg ggcagtttcc cgcggcgggc
     1681 gctgcggctt gtgcaggcgt ggcgcgagct ccatcgcgac cttaacccaa gccaccggcg
     1741 cgcggcgtac atcacacatc aaaatacttc gcctgcgggt ggtgcacgac cagcgcatcg
     1801 gtgctctgct ccggcacgag catgaagttc tcgctcaagc tcacgccgat cgaatcgggc
     1861 ttcagcagct cgacgatctt cgcgcggtct tccaggttcg ggcaggcgcc gtagccgaag
     1921 ctgaatcggc tgccgcggta gccttgttgg aacagctggg cgatcttcgg cgcgtcttcg
     1981 ctgccgaagc ccagctcctg gcgcatgcgc ttgtgccaca gctcggccag gccttcggcc
     2041 gactcgacgc cgaagccgtg gaggtagaga tagtcctggt atttgttggc cttgaacagt
     2101 tcctgggcca gctcggcggc gcgatcgccg acggtgacca gctgcacgcc gagcacgtcg
     2161 tactggccgg attccttcga gcggaagaag tcggcgacgc acagccgccg gcgggtgttc
     2221 tgccggggaa aggtgaagcg catgcgctcg cgcggcgcgc ctgccggttg cagtttgcca
     2281 gcgctggcgc cgtgctggcc gcaagcgcag ccggcgaatt cttcggtgtg gtagacgaca
     2341 atatcgtccc cttcgctctg cacggggaag tagccgtaga cgactttcgg cgtcagcagt
     2401 ttctcggcga tcgcgcggcg ttgcagctcc gcgtacaccg gccgggcctt atcatccaat
     2461 agccgctcgt acgcctcgtc gctcagtccc ttcttcttga aatcccactg ccccaggaac
     2521 agcgcgttgg gattgatgta cgcgaacacc tgctccagcg gaatatcttt gacctcgcgc
     2581 cgcccccaga acggcggcgt gggcacgggg ttgtccgtct tcacgtcgct cttggccggc
     2641 agcgttgaaa cgtcttcgcc cttgtacccc ttggtcgcgc gcagctccgc acgccgcgcg
     2701 ccatcggcct gctgcttcga gaacgcgtct tccagatgcc cgccgacgac ggcgtccatc
     2761 gtgttcaaac ccgcaaacgc gtccttgcag taaaacagct tgcccttgta cagcgccgcc
     2821 aattcgttga cggcgtaatc cttcgtcagc gccgctccac ccagaagaac aggaacgtcg
     2881 atccccaccg cgttcatctc ttccaggttt tccttcatca cgccgacgct cttgaccagc
     2941 agtccgctca tgccgatcgc gtcggcggac ttcgccttcg cttccttcaa aatatcgctc
     3001 actggctgct tgatgccgag gttgtaaacg gtgtaaccgt tgttggtcag gatgatgtcg
     3061 accaggttct tgccgatgtc gtgcacgtcg cccttcacgg tggcgagcac gatcttgccc
     3121 ttggtcgagc cttcgctctt ctccatgaac ggctcgagat acgcgacggc cgccttcatc
     3181 gtctcggcgc tctgcagcac gaacggcagc tgcatctgcc ccgaaccaaa caaatcgccg
     3241 acaactttca tgccgtccaa aagatgatcg ttgatgatcg ccagcggcgg atatttctgg
     3301 cgcgcttcat ccaggtctgc gatgaggttt cgcttctcac cgtcgatgat gtgacggcgg
     3361 agtttctcct caaccggcgc atctgccgct tgcggcttag cgtccgttgg tccccccgcg
     3421 tcatctggaa acagggagat gaaatgagtg agcggatcaa acccctcgcg ccgccggtca
     3481 taaatcagat ccagcgccgc atcccactgc tcctgcggaa tgcgattctt cgggaggatt
     3541 tttgacgcat gtacgatcgc gccagtcagt cccgcttcgc gtagctcatg caaaaacgcg
     3601 ctgttgagca ccacccgcgc cgccggtttc aggccgaacg acacgttcga caaccccaac
     3661 accgtgtggc acctgggcag ctcctgcgaa atccgccgcg tcccctcgat cgtctccagc
     3721 gcgttgcggc gatcttcctc gatccccgtg ctgatcggca gcaccagcgg atcgaacatc
     3781 agatcttcat cgcgaagccc gaaccggccg accgcgagat cgcggatgcg cgtagcgatc
     3841 gcgatcttcc gctcgcgggt gcgggccatc gcctgctctt tgtcctcgtc gatgcagccg
     3901 gcgacgacgg c
//
LOCUS       DATIMH010000924         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3108614, whole genome shotgun sequence.
ACCESSION   DATIMH010000924 DATIMH010000000
VERSION     DATIMH010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3108614"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..545)
                     /gene="hfq"
                     /locus_tag="VLI90_16055"
     CDS             complement(288..545)
                     /gene="hfq"
                     /locus_tag="VLI90_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HSV15776.1"
                     /translation="MATERSQNLQDTFLNYVRKQKTPLTIFLVNGVKLQGVVTWFDNF
                     CVLLRRDGHSQLVYKHAISTIMPGHPIQLFEGAEEPPAEKV"
     gene            complement(727..>1537)
                     /locus_tag="VLI90_16060"
     CDS             complement(727..>1537)
                     /locus_tag="VLI90_16060"
                     /inference="COORDINATES: protein motif:HMM:NF005209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HSV15777.1"
                     /translation="AMVHIEDRGYQFSDGVYEVCEVRAGRLVDERRHIERLQRSLGEL
                     RIRPPMSPLALGVVMRETIARNRVRNGIVYLQVTRGVARRDHAFPAAATAPSLVVTAR
                     SISIAGNERTAAAGIAVISVPDNRWGRVDIKTVGLLPNVLAKQAARERGAREAWFVDR
                     EGYVTEGSSTNAWIVTAGGKVVTRPADSAILRGITRTVLIEAIAAQGLELEERPFTLS
                     EAYAAREAFVTAASQIVMPVVRIDGRAIGGGVPGPIARALRARFHGHAELG"
BASE COUNT          254 a          511 c          493 g          279 t
ORIGIN      
        1 ataccggcga gctcctcgac cttgcccttg ccgaggaaag tcgccggccg cacgttggcg
       61 agcggcacca cgccggattg cacgatcgtc aggtcgatgg cgcgggcaag gccgacagct
      121 tcttccagcc gcgcatcggg cgagcgcaac gccaatcgcg cacgcgcatc cgctccgccc
      181 ggcgtcctgc cgcgcagatg cggttcaaca acgatcgcgc gtccggtgcc cgaactctct
      241 cccaagatag ggcgatcagc gcgcgtatcg aaccggcggg gttccaatca gactttttcc
      301 gccggaggct cttcggcccc ttcgaacagc tggatcggat gacccggcat gatcgtcgag
      361 atcgcgtgct tgtagaccag ttgggaatga ccatcgcgtc gcagcagcac gcagaaatta
      421 tcgaaccagg tcaccacgcc ttgcagcttg acgccgttca ccaggaagat cgtgagcgga
      481 gttttttgct tgcggacgta gttcaggaag gtgtcttgga gattttgtga ccgttcggtc
      541 gccattgtgg tgttccgttt tttcctcgcg ggccccttcc cgcaagactt attgccgggt
      601 cgctcggtcc ctcgttgttc gctgtcgcta ccggggaccc ggtccgggct attacattgc
      661 acgttttcgg gctgtgcaag cataagttgg gggcaacgcg tgtctaatcc ggcgaagttg
      721 ggccgctcac cccaattcgg cgtggccatg aaacctggcc cggagcgccc gggcgatcgg
      781 ccccgggacg ccaccaccga ttgcacggcc gtcgatgcgc accaccggca tgacgatctg
      841 gctcgccgcc gtcacaaagg cttcgcgggc ggcataggcc tctgacagcg tgaaaggccg
      901 ctcttccaac tcaagcccct gcgccgcgat cgcctcgatc agcaccgtgc gggtgatgcc
      961 acggaggatg gcgctgtcgg ccggccgggt gaccaccttg ccccccgcgg tcacgatcca
     1021 ggcattggtt gaggagccct ctgtcacgta gccctcccgg tcaacaaacc aggcctcgcg
     1081 ggcgcctcgc tcgcgggccg cctgtttggc gagcacattg ggcaagagcc cgacggtctt
     1141 gatgtcgacg cgcccccaac gattgtccgg aaccgaaatg acggcgatcc cggcagcggc
     1201 ggtcctttcg ttcccggcaa tgctgatgct gcgcgccgtg acgaccaggc ttggcgcggt
     1261 tgcggcggct ggaaaggcgt ggtcgcggcg ggcgacgccg cgggtcacct gcagatagac
     1321 gatgccattg cgtacgcggt tgcgcgcgat cgtctcacgc atgacgacgc caagggccaa
     1381 gggcgacatc ggcgggcgga tgcgcaattc gccgagcgag cgctgcaggc gctcgatgtg
     1441 gcgccgttcg tcgaccaggc gccctgcccg cacctcgcac acctcgtaga cgccgtcgga
     1501 aaactgatag ccgcgatctt cgatgtgcac catcgcg
//
LOCUS       DATIMH010000925         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2529962, whole genome shotgun sequence.
ACCESSION   DATIMH010000925 DATIMH010000000
VERSION     DATIMH010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529962"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VLI90_16065"
     CDS             complement(<1..230)
                     /locus_tag="VLI90_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15778.1"
                     /translation="MVSRSIQTDFEFLGFGVLTTWQIALTPLRTQASYIPCWAIVVLT
                     AILPARWMRHRRLPEAGRCRRCGYDLCATPER"
     gene            638..964
                     /locus_tag="VLI90_16070"
     CDS             638..964
                     /locus_tag="VLI90_16070"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSV15779.1"
                     /translation="MVATILLAALASTTQPNVANSADNAGLDAELSPPVRIEAAGKPI
                     DTETGHAAPFVADFDGDGVPDLLVGQFGNGQLLIFHNDGSAAAPRFAKAQLFKDGADT
                     GRVPSG"
     gene            989..2029
                     /locus_tag="VLI90_16075"
     CDS             989..2029
                     /locus_tag="VLI90_16075"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSV15780.1"
                     /translation="MDLDGDGIPDLISGSWPGEIYFLKGRGHGQFDDKIKLKDKNGKL
                     INIGGGVQRNDDTQLMIAGDATFENKDGKSIIVYDGQRFDATGKQGGITGTASAVYAF
                     DLRNTGRFDLIVGDVMGNVWFVPNDGTPKSRAFGDAQHLRAGGEDIHVDGDAGPTIAD
                     WDGDGKPDLLLGSGDGSVRLFRNSGNVDPQTKLPIFEPGRILVPPTQQHMADPPATPS
                     RGIRSKICVADVNGDGRPDLLVGDFAFQKPPTTPPTPQQQAEYNKLRAQMNEIQPKYS
                     TAVQKLYPRDPSQPKLTAKQRQEVEKEFSQLAQQMTSIREKLPPETDYHGWVWLFTRK
                     PAATAGAAAAAP"
     gene            complement(2056..>2276)
                     /locus_tag="VLI90_16080"
     CDS             complement(2056..>2276)
                     /locus_tag="VLI90_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15781.1"
                     /translation="ASSVGSALNGEPRARTLLVWDGIDVGQQPGAEDLLPRLGDGWRL
                     ASVELYPVRMFLDWGKLYTNRRRVYVRR"
BASE COUNT          483 a          808 c          666 g          319 t
ORIGIN      
        1 cagcgctctg gcgtggcgca gaggtcgtag ccgcagcgcc tgcagcggcc agcttccggc
       61 agccgccggt gcctcatcca ccgcgccggc agaatggctg tcaggacaac aattgcccaa
      121 cacggaatgt aggacgcttg agtgcgaagc ggcgtgagtg ctatctgcca ggtcgtgagc
      181 acgccgaagc ccagaaactc gaagtcggtt tgaatcgagc gcgacaccat cgacctgtac
      241 catccggaat gggaagcggc tcaccccaat gaaacaagaa cccgcggcgc gttaagggga
      301 gaccgctcac gtgggcgaac tcaatctcgc ctcgcgagat ctgtatggac caaacgatgt
      361 cggcaggatc ccactccacg accggagcca caaaataact catgacccaa aatgctgcgg
      421 tggcgaggca catcgccagt gagacgggcg ttatcacgtt gaagagacga cgcttcacca
      481 cggacatcgt accgcgcggg ggcggcggac ggaggcgcgg accgccttca gctgcggcgg
      541 ggggcggcgg ccaggtgttc cacctagcta tttctaaaat tctccccacc cccgctcacc
      601 cgttgtgtat actccctccc caccggaggc accctccatg gtggcaacca ttctcctggc
      661 ggccctggcg agcacgaccc aaccgaacgt cgcgaacagc gccgacaacg ccggcctcga
      721 cgccgagctt tccccacccg tacggatcga agccgcgggc aaaccgatcg acaccgagac
      781 cggtcacgcc gccccgttcg tcgccgattt cgacggcgac ggtgtgcccg acctgctcgt
      841 cgggcagttc ggcaatgggc agctcttgat cttccacaac gacggctccg ccgccgcccc
      901 gcgcttcgcc aaggcgcaac tcttcaagga cggcgccgac accggccgcg tgcccagtgg
      961 ctgatgcatc ggctttggtc cacagttggt ggacctcgac ggcgacggca tccccgacct
     1021 gatctccggc tcctggccgg gcgaaatcta cttcctcaag ggccgcggac acgggcaatt
     1081 tgacgacaag atcaaactca aagacaagaa cggcaagttg atcaacatcg gcggcggcgt
     1141 gcagcgcaac gacgacaccc agctcatgat cgccggtgac gccacgttcg aaaacaaaga
     1201 cggcaaatcg atcatcgtct acgacggcca gcgcttcgac gccaccggca agcaaggcgg
     1261 catcaccggc acggcatccg ctgtgtatgc cttcgatctc cgcaacaccg gccggttcga
     1321 cctgatcgtt ggcgacgtca tgggcaacgt ctggttcgtc cccaacgacg gcacgccaaa
     1381 gtccagggcc ttcggcgatg cccaacacct gcgcgccggc ggcgaagata ttcacgttga
     1441 cggcgacgcc ggcccaacga tcgccgactg ggacggcgac ggcaaaccgg acctgctgct
     1501 cggcagcggc gacggcagcg tgcgcctctt ccgcaacagc ggcaacgtcg atccgcaaac
     1561 caagctgccg atcttcgaac ccggccgcat cctcgtcccc ccaacgcaac agcacatggc
     1621 cgacccgccg gcgacgccgt cgcgcggcat ccgctcaaag atctgcgtcg ccgacgtgaa
     1681 cggcgacggc cgccccgatc tgctggtcgg cgacttcgcc ttccagaaac cgccaacgac
     1741 gccgcccacc ccgcagcagc aggccgagta caacaagctc cgcgcccaga tgaacgagat
     1801 ccaaccgaag tattcgacgg cggtgcagaa gctgtacccg cgcgatccaa gccagccaaa
     1861 gctaaccgcg aaacagcgcc aagaggtcga gaaggagttc tcgcagctcg cgcagcagat
     1921 gacgtcgatc cgagaaaagc tgccgccgga aacggattat cacgggtggg tatggctgtt
     1981 cacgcgcaaa cccgcggcga cggcaggagc tgccgcagcg gcgccgtgat tgaaaagaaa
     2041 gcgcgccact cgccatcacc gccgcacgta aacgcgccgg cggttcgtgt acagctttcc
     2101 ccaatccagg aacatccgaa ccggatacaa ctccaccgac gccagccgcc aaccatcgcc
     2161 cagccgcggc aggagatctt ccgcgcccgg ctgctggccg acgtcgatgc cgtcccatac
     2221 cagcagcgtc cgcgcacgcg gctcgccgtt cagcgcggaa cccacacttg acgcat
//
LOCUS       DATIMH010000926         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2819186, whole genome shotgun sequence.
ACCESSION   DATIMH010000926 DATIMH010000000
VERSION     DATIMH010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2819186"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..985)
                     /locus_tag="VLI90_16085"
     CDS             complement(53..985)
                     /locus_tag="VLI90_16085"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HSV15782.1"
                     /translation="MSDERGVSTAQDSSFIAPRSSFALIVTADDFGIGRRTSEGIIKA
                     HLHGPVTATSLMSMTGDHVRASVPLLADAPNLDVGLHLVLTNCGEKPLMARYSSGLIG
                     RDGLFHPNGKLWIKALTGKLQRAGIADEIVAQAEMFHKLVGRPPAYVDCHHHAHQLPI
                     VRDALLDIIALGLLPPITRVTVEPPGLIRRVGSVRLKRTAAQWMGRRAAKAFDAKWIW
                     TNDRYFGMLAPRDLRRAFPWAKYLAKLPQSGVVEWVVHPGEADDTLHGRDGYRTERVK
                     ELEALTSADGNRSWQHLRPFLSRKSILHRKPVEA"
     gene            complement(1002..1835)
                     /locus_tag="VLI90_16090"
     CDS             complement(1002..1835)
                     /locus_tag="VLI90_16090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV15783.1"
                     /translation="MSIDLDLNDFAAAAAASAEPQFNCTLILPTYNASNFIEATVTKL
                     VRFLSHHEDWCVLFVCDGCSDDTVAKLAALTEGFDPHMRVESYEKNRGKGYALRRGLS
                     VANTPYKIFTDCDLAYDPEESIKILKLLEGGADLAVVNRASPDSQYLISPRDFPNIYK
                     RHLMSRSFNWWLRQMLPITILDTQAGLKGLTAVAWERLAPQMTTDGFFFDVELLARAG
                     AAGLRIDEAPVFFHYVDPTTVRMVNHGWRMIKDTLNLRRNMRRTKRPVITRVVPAVSA
                     L"
BASE COUNT          403 a          655 c          686 g          405 t
ORIGIN      
        1 aagaccaaaa gaccggggct tccgcccccg gctattaatg cgttttctat gttcacgctt
       61 caaccggctt tcgatgcagg atcgacttgc gtgacaggaa cggacgaagg tgctgccaac
      121 tgcgatttcc gtcggccgag gttagtgctt ccagctcctt cacgcgctcc gtgcgatagc
      181 cgtcgcggcc atgcagggtg tcatccgcct caccgggatg aaccacccat tcgacgacgc
      241 cggactgcgg cagcttcgcc aggtacttcg cccatgggaa cgcccgccgc agatcgcggg
      301 gcgcaagcat gccgaagtag cggtcgttgg tccagatcca tttggcgtcg aacgccttgg
      361 cggcgcgccg gcccatccat tgagcggccg ttcgtttcag gcgcaccgag ccgacgcggc
      421 ggatcaaccc gggcggttca accgtcacgc gcgtgatcgg cggcagcagg cccagcgcga
      481 tgatgtcgag cagcgcatca cgaacgatcg gcaactggtg ggcgtgatga tggcaatcaa
      541 catacgccgg cgggcggcca accagcttgt gaaacatctc cgcctgcgcg acgatctcat
      601 cggcgatgcc ggctcgctgc agcttgccgg tcagcgcttt gatccacagt tttccgttgg
      661 ggtggaacag accgtcgcgg ccgatcaggc ccgatgaata gcgggccatc agcggcttct
      721 caccgcagtt ggtgagcacc aggtgcaggc cgacgtcgag gttcggcgca tcggccagca
      781 gcggcaccga ggcgcgcacg tgatcgccgg tcatcgacat caagctggtc gcggtgaccg
      841 ggccgtgcag gtgggccttg atgatcccct cgctggtccg ccggccgatg ccgaagtcgt
      901 cggcggtgac gatcagcgca aacgatgaac gtggagcgat gaacgatgaa tcctgtgctg
      961 tgctcactcc gcgttcatcg ctcatcgtgc atcgatatcc gttacagcgc cgacaccgcc
     1021 ggcaccacgc gggtgatgac tggccgtttc gtccggcgca tgttgcggcg gaggttgagc
     1081 gtgtccttga tcatccgcca gccgtggtta accatgcgga cggtggttgg atcgacgtag
     1141 tggaagaaga ccggcgcttc gtcgatgcgc agtcccgcgg cgccggcgcg ggcgagcaac
     1201 tcgacgtcga agaaaaaccc gtcggtcgtc atctgcggcg ccagccgttc ccacgcgacg
     1261 gcggtcagac ccttcagccc agcctgcgtg tcgaggatgg tgatcggcag catctgccgc
     1321 agccaccagt tgaagcttcg gctcatcagg tggcgcttgt agatgttggg gaaatcgcgc
     1381 gggctgatga ggtactgcga atccggactg gcgcgattga ccaccgccag gtccgccccg
     1441 ccttcgagca gcttcaggat cttaatcgac tcttccggat cgtaagccag gtcacagtca
     1501 gtgaagatct tgtacggggt gttggccacg ctcagcccgc ggcggagggc ataaccctta
     1561 ccgcggttct tctcgtatga ctcgacgcgc atgtgcggat cgaagccttc ggtcagcgcg
     1621 gcgagcttgg ccacggtgtc gtcgctgcag ccgtcgcaga cgaacaggac gcaccagtct
     1681 tcgtggtgcg acaggaagcg cacgagcttg gtgacggtgg cttcgatgaa attcgacgcg
     1741 ttgtaggtcg gcaggatcag cgtgcagttg aactgcggct cggccgacgc cgccgcggcg
     1801 gctgcgaaat cgttcagatc cagatcgatt gacataaata acgcccgtgc aaggcaaaac
     1861 ccgcgtgcgt cgcggcgcgt cgcctttgga ccgataacac acgcagtttt agtggagtat
     1921 cggctgaaag tctagaaaac gagtagtcaa acgccgggcg gaagcgttcg gttcggttcg
     1981 tttagcggcc gcgttaacgc ggccgctaaa ggaggggcgg attcacatcc ttgacggatc
     2041 aaaaccggtt cacaacaatt cgccccttat gcctcgcacg atcaatcacc cggcccttca
     2101 gccgttgaaa gccaaattct cggaagtgaa gttcctcgtc ggcgaattt
//
LOCUS       DATIMH010000927         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3969891, whole genome shotgun sequence.
ACCESSION   DATIMH010000927 DATIMH010000000
VERSION     DATIMH010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3969891"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2469
                     /locus_tag="VLI90_16095"
     CDS             <1..2469
                     /locus_tag="VLI90_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSV15784.1"
                     /translation="SLMISPTESRRTDRLEFSPIPVNQYNRSVSDELASGKFTKADLL
                     RIQRDMEVIRAFENMLNEIKLRGNYKGIEYNHRGPAHLSIGQEASAVGQAYLLGVDDH
                     IYGSHRSHGEILAKGLSAIHKLNDDALLGIMKNYFGGDAYRVVEKDAKGSTKDLATDF
                     LVYGALAEIFARDYGFNKGVGGSMHAFFPPFGIMPNNAIVGGSGDISVGAALFKHVNK
                     KPGIVIANIGDASAGCGPVWEGICFATMDQFKSLWEESHRGGLPLIINFVNNFYGMGG
                     QPVGETMGFKVLARLGAGVNPEQMHAERIDGYSPLAVIDAIARKKRILEDGDGPVLLD
                     TITYRFSGHSPSDASSYREKAEVEAWQKEDSLITFAHNLIEAKVATAADLDAIKAGAE
                     ELIFKAFKKAVDLNISPRSTFKNTQNFLEKAMFSNQSIDSLDTKRKPEVLLSKEDNPR
                     VKQLAQRSRSATDDKGAKLPKSKTIGIRDALFEAIFDQFYKDPTLIAYGEENRDWGGA
                     FAVYRGMTEALPYHRLFNSPISEAAIIGTAVGYALEGGRALVELMYCDFIGRAGDELF
                     NQLAKWQSMSGGLLKMPVVVRVSVGSKYGAQHSQDWSSMCAHIPGLKVVFPATPYDAK
                     GLMYSALKGTDPVIFFESQRIYDEPEHFAKGGVPTGEYEVPMGEPDVKKVGEDLTILT
                     IGATLYRALEAAKQLEEKWGISCEVIDARSIVPFNFAKVVESVKKTRKILLASDACER
                     GSALQTFASKITQFAFDELDAPPVVVGARNWITPADEVEDAFFPFPVDIIDAVHEHIM
                     PLKGYQVSRENSTQDLLRRSAEGV"
     gene            complement(2510..3100)
                     /locus_tag="VLI90_16100"
     CDS             complement(2510..3100)
                     /locus_tag="VLI90_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV15785.1"
                     /translation="MEIELSEEPISGLRQQADISIAFEVDRLFDVEPRANAATGFRLA
                     ERSVATPYTKDYDAIDGEGPGRWAERFDVSKWGYIVARLDGHRVGGAVIAFDTAGVEM
                     LEGRRDLAVLWDVRVCPGLRGSGVGSALFEAAETWAAARGCRQIKVETQNINARACRF
                     YAKHGCTLRRVERLAYAQFPDEVRLLFYKDITAACS"
     gene            complement(3104..>3297)
                     /locus_tag="VLI90_16105"
     CDS             complement(3104..>3297)
                     /locus_tag="VLI90_16105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV15786.1"
                     /translation="GVIDTEMTRGVPEEIARQYLQQIPLGRVGTVDDIASTVLFLVSA
                     GAGYITGQVIHVNGGFYMG"
BASE COUNT          686 a         1007 c         1006 g          598 t
ORIGIN      
        1 agtttgatga tcagccccac cgagtcgcgt cggaccgacc ggctggagtt cagccccatc
       61 ccggtgaacc agtacaaccg cagcgtcagc gatgagctgg cgtccggcaa atttaccaag
      121 gccgacctgc tgcgcatcca gcgcgacatg gaggtcatcc gcgcgttcga gaacatgctg
      181 aacgagatca agctgcgcgg gaactacaaa ggcatcgaat acaaccaccg cgggccggcg
      241 catctgtcga tcgggcagga agcgtcggcg gtggggcagg cgtatctgct gggggtcgat
      301 gatcacatct acggctcgca ccgcagccac ggtgagatcc tggccaaggg tttgtcggcg
      361 atccacaagc tgaacgacga tgcgttgctc ggcatcatga agaactattt cggcggcgac
      421 gcctaccgcg ttgtcgaaaa ggatgccaaa ggatcgacca aggatttggc gacggacttc
      481 ctcgtctacg gcgcgctggc ggaaatcttc gcgcgtgatt acggcttcaa caaaggcgtg
      541 ggcggatcga tgcacgcgtt cttcccgccg ttcggcatta tgccgaacaa cgcaatcgtc
      601 gggggctcgg gtgacatcag cgttggcgcg gcgctgttca agcacgtcaa caagaagccc
      661 ggcatcgtca tcgccaacat tggtgacgcg tcggccggtt gcgggccggt ttgggaaggc
      721 atctgcttcg ccaccatgga ccagttcaag agcctgtggg aagagagcca ccgcggcggg
      781 ttgccgctga tcatcaactt cgtcaataac ttttacggca tgggtggcca gcccgtcggt
      841 gagacgatgg gcttcaaggt gctggcccga ctcggtgccg gcgtgaaccc cgagcagatg
      901 cacgccgagc gcatcgacgg ttacagcccg ctggcggtga tcgatgcgat cgcgcgcaag
      961 aagcgcatcc tggaagacgg cgacgggccg gtgctgctcg acacgatcac gtatcgtttc
     1021 tccggccact cgcccagcga cgcgtccagc tatcgcgaga aggccgaggt cgaagcctgg
     1081 cagaaggaag attcgctgat caccttcgcg cacaacctga tcgaagccaa ggtcgcgacg
     1141 gcggctgatc tggatgcgat caaagccggc gcggaagagc taatcttcaa ggcgttcaag
     1201 aaggcggtgg acctgaacat cagcccgcgc tcgacgttca agaacacgca gaatttccta
     1261 gaaaaagcga tgttttcgaa ccagtcgatc gactcgctcg acaccaagcg caagcctgag
     1321 gtgctgctga gcaaggaaga taacccgcgc gtgaagcagc tggctcagcg cagtcgatcc
     1381 gccaccgacg acaagggcgc gaagctgccg aagagcaaga cgatcggcat ccgcgatgcg
     1441 ctgttcgaag cgatcttcga tcagttttac aaggacccca cgctgatcgc ctatggcgaa
     1501 gagaaccgcg attggggcgg ggcgttcgcc gtctatcgcg ggatgaccga agcgctgccg
     1561 tatcatcggc tgttcaattc acccatcagc gaagccgcca tcatcggcac cgccgtgggc
     1621 tatgcattgg aaggcgggcg agcactcgtc gagttgatgt actgcgattt catcggccgc
     1681 gcgggcgacg aactgttcaa ccaattggcc aagtggcaat ccatgagtgg tgggctgctg
     1741 aagatgccgg tggtcgtgcg cgtcagcgtc ggcagcaagt atggcgctca gcacagccag
     1801 gactggtcca gcatgtgcgc ccacatcccg ggcctcaaag tcgtcttccc ggcgacgcca
     1861 tacgacgcca aggggttaat gtactccgca ttaaagggga cggaccccgt tatcttcttc
     1921 gaaagtcagc gcatctacga tgagcccgaa catttcgcca aaggcggggt gccgactggc
     1981 gaatacgaag tgccgatggg cgagccggac gtgaagaagg taggcgagga cctgacgatc
     2041 ctgacgatcg gcgcgacgct gtatcgcgcg ctggaggcag ccaagcagct ggaagagaag
     2101 tggggcatca gctgcgaggt gattgatgcc cgctccatcg tgccgttcaa cttcgcaaag
     2161 gtggtcgaga gcgtgaagaa gacgcggaag attctgctgg ccagcgacgc ctgcgaacgc
     2221 ggcagcgcgc tgcagacgtt cgcgtcgaag atcacgcagt ttgcgttcga cgaactcgac
     2281 gccccgccgg tggttgtcgg tgcgcgaaat tggatcacgc cggctgacga agtggaagac
     2341 gcgttcttcc cgttcccggt cgacatcatc gacgcggtgc atgagcacat catgccgctg
     2401 aagggttacc aggtgagccg cgagaactcg acgcaggatc tgctccgccg cagcgctgag
     2461 ggtgtttgat aaaccgatca gcggcgtgct cgcttcagcg accatgtctt cagctgcacg
     2521 ccgccgtgat gtccttgtaa aaaagcaacc gtacctcatc tggaaattga gcgtatgcca
     2581 gccgctcaac ccttcgaagc gtgcacccat gctttgcata aaaccggcac gcccgcgcgt
     2641 tgatgttttg cgtttccact ttgatctggc gacaacctct cgccgctgcc cacgtctcgg
     2701 cggcctcgaa tagcgccgat ccgactccgc tgccgcgcag ccccggacaa actcggacgt
     2761 cccacaagac ggccagatcc ctgcgtccct ccaacatctc cacgcccgcc gtgtcgaacg
     2821 cgatgaccgc tccgccgact cggtggccgt ccaggcgagc tacgatgtat ccccatttcg
     2881 aaacatcgaa gcgctcggcc caacggcccg ggccttctcc atcgattgcg tcgtagtcct
     2941 tcgtatatgg agtcgcgacg gagcgttccg ccaaacggaa tccggtcgct gcgttagccc
     3001 tcggctcgac atcgaacagc cgatcaactt cgaatgcgat tgagatgtcc gcctgctgcc
     3061 ggagaccact gatcggttcc tcgcttaatt cgatctccat ggctcatccc atgtagaacc
     3121 caccgttcac gtggatgact tgcccggtga tataccccgc gccggctgag acgaggaaca
     3181 gcacggtgct ggcgatatca tcaaccgtgc ccacgcgtcc cagcgggatc tgctggaggt
     3241 attgccgggc gatctcctcc ggcaccccgc gagtcatctc cgtatcaatt acgcccg
//
LOCUS       DATIMH010000928         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5423405, whole genome shotgun sequence.
ACCESSION   DATIMH010000928 DATIMH010000000
VERSION     DATIMH010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5423405"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /gene="mnmA"
                     /locus_tag="VLI90_16110"
     CDS             complement(<1..789)
                     /gene="mnmA"
                     /locus_tag="VLI90_16110"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HSV15787.1"
                     /translation="MPTPKKKVVLAMSGGVDSSVAAALLLRAGYEVLGVFMRLGSPDG
                     VEHESTESCDPTAGKNKQGCCSVLDAADARRVAGLLDIPLYVLNFQDEFGRVIDYFVA
                     EYNRGRTPNPCVRCNDWLKFGRLAKYAEAVGADYVASGHYARIGVDPITGKKALLRGL
                     DHRKDQSYVLFGMNERTIEHTLLPIGEYEKPAVRAIAEELKLPVFNKPDSQEICFVPN
                     QDYAGLVQRRSPETFRQGELVTSTGQTVGTHAGHQHFTIGQRKGV"
     gene            980..2809
                     /locus_tag="VLI90_16115"
     CDS             980..2809
                     /locus_tag="VLI90_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HSV15788.1"
                     /translation="MLGRLGLGEDAFLLLLAIIIGTITGVAAVSFHELINFIRDQLYA
                     SVTHQFLYGKGVFLLVIYPALGGLAVSVTSKYVFKTREGHGIVDVVESVVRSSGFQKP
                     TTAVEKILTSAFTIGSGGSAGAEGPIVQIGAAIASGVGLMFRMARAQMPILTGCGCAA
                     GISAIFNAPFGGVLFTLEVILQDFSIRSFTPVVLASVIAQVTVQATFQLMHHATEYRA
                     IFAMPAVEVVRHSMLNWEQVGNFVLLGIVCGVIGSAFTRMMYWTEHSISLMKLPKVMR
                     PALGGAALGFIGIVYIWLFGHLLLDVPNNKPFPHYPMPAFFGDGYGVIQDMLEASFYT
                     HMGGKVMLLVLFLCAAKIVGTCLTLGSGGSGGVIAPSVFIGATTGAVVGHVLQRTGWF
                     HGLQPELYALVAMGAVLAAVVHAPMASILIVFELTQDYKVMLPAMLACIVAVAVARLC
                     YPESVYTLSLRTRGVRVGSAGDFVLLRRINVEQVALIPVSVVHESDPLQQLIELSAMT
                     STYDFVVADEKGKYEGMVYSTDLSPVLMDREAVPVLVVRDLMRRDIPPVRTTDDLAAV
                     FDAFSRYDVNHLPVSMADSRDKVIGMISRSVVIRKYQEALKGT"
BASE COUNT          470 a          846 c          978 g          587 t
ORIGIN      
        1 gacgcctttg cgttggccaa tcgtgaaatg ctgatgcccc gcgtgcgtgc cgacggtttg
       61 gccggtgctc gtcaccagct cgccttggcg gaacgtctcg ggacttcgcc gctgcaccag
      121 ccccgcgtaa tcctggtttg gcacgaagca gatctcctgg ctgtcgggct tgttgaagac
      181 cggcagcttc agctcttcgg caatcgcgcg cacggcgggc ttttcgtatt cgccgatcgg
      241 cagcagcgtg tgctcgatcg tccgctcgtt catgccgaac aggacgtagc tctggtcctt
      301 gcgatgatcg aggccgcgca gcagcgcttt ctttcccgtg attggatcga cgccgatgcg
      361 ggcgtagtgc ccgctggcaa cgtaatccgc gccgacggct tcggcgtact tcgccaatcg
      421 tccgaacttc aaccagtcgt tgcagcgcac gcacggattg ggcgttcgcc cgcggttgta
      481 ttcagcgacg aagtagtcga tcacgcggcc gaattcgtcc tggaaattca acacgtacag
      541 cgggatgtcg agcagccccg cgacccgccg ggcgtcggcg gcgtcgagga ccgagcagca
      601 gccctgcttg ttctttcccg cggtgggatc gcacgattca gttgattcgt gctcgacgcc
      661 atcgggcgaa ccgagccgca tgaagacgcc gagcacttcg tagcccgcgc gcagcaagag
      721 cgccgcggcg acggagctgt cgacgccgcc gctcatcgcg aggacgactt ttttctttgg
      781 tgtaggcatg atgcgtggtt ttcgaacggc attctacggt gcatgtgggg gtccgtccaa
      841 agaatcggtg ggacatgctc gctgaagcga ccacgccgcg cgggtggttc gcgtcagcgc
      901 ggcttctcgt tatgctctga cggccatgac tctacgattg ggcgcgcccg gcggacggtt
      961 gcgcatcgtt gcaactcgaa tgcttggacg cctgggtctg ggggaagatg cgttcctgct
     1021 gctgctggcg atcatcatcg gcacgatcac cggcgtggcg gccgtcagct ttcacgagtt
     1081 gatcaacttc atccgcgacc aattgtacgc gagcgtgacg caccaatttt tgtacggcaa
     1141 aggcgtcttc ctgctggtga tctacccggc gctgggtggg ctggcggttt cggttaccag
     1201 caagtacgtc ttcaagaccc gcgaggggca cgggatcgtc gatgtcgtcg aatccgtcgt
     1261 gcgcagcagc gggtttcaga agccgacgac cgccgtcgag aagatcctca ccagcgcgtt
     1321 caccatcggc agcggtggca gcgcgggcgc ggaagggccg atcgtgcaga tcggcgccgc
     1381 catcgcgtcg ggggtcgggc tgatgttccg catggcccgt gcgcagatgc cgatcctcac
     1441 cggttgcggg tgtgccgcgg gcatcagcgc gatcttcaac gcgccgttcg gcggggtgct
     1501 gttcacgctg gaagtgatcc tccaggattt ttcgatccgc tcgttcacac ccgtggtgct
     1561 ggccagcgtc atcgcgcagg tgacggtgca ggcgacgttc cagctgatgc atcacgcgac
     1621 agagtatcgc gcgatcttcg cgatgccggc ggtggaagtc gttcggcact cgatgttgaa
     1681 ctgggagcag gtcggcaact tcgtcctgct gggcatcgtc tgcggcgtga tcggctcagc
     1741 cttcacgcgc atgatgtact ggaccgagca ttccatctcg ctgatgaagc tgccgaaggt
     1801 gatgcggccg gcgctgggcg gcgcggcgct gggcttcatc ggcatcgtgt acatctggtt
     1861 gttcggccac cttctgctgg acgtgccgaa caacaagcca tttccgcatt atcccatgcc
     1921 ggcatttttc ggcgacgggt acggcgtcat ccaggacatg ctggaggcca gcttctacac
     1981 ccacatgggc gggaaggtca tgctgctggt gctgttcctg tgcgccgcga agatcgtcgg
     2041 cacgtgcctg acgctgggca gcggcggcag cggtggcgtg atcgcgccca gcgtgttcat
     2101 cggcgcgacg accggcgccg tggtcggcca cgtgctacag cgcaccgggt ggttccatgg
     2161 gctgcagccg gagctgtatg cgctggtggc gatgggcgct gtgctggccg cggtggtgca
     2221 tgcgccgatg gccagcatct tgatcgtgtt tgagctgacg caggactaca aagtgatgct
     2281 gccggcaatg ctggcctgca tcgtcgccgt cgccgtcgcg cggctgtgct atcccgaatc
     2341 ggtctacact ctgagcctgc gcacccgcgg cgtgcgcgtc ggttcggccg gcgatttcgt
     2401 gctgctgcgg cggatcaacg tcgagcaggt ggcgctgatt cccgtctcgg ttgtgcacga
     2461 gtccgatccg ctgcagcagc tgatcgaact gagcgcgatg accagcacgt acgacttcgt
     2521 cgtcgctgac gagaagggga agtacgaggg gatggtttac tcgacggatt tgagcccggt
     2581 attgatggac agggaagcgg tgccggtgct ggtggtgcgc gacctgatgc ggcgcgacat
     2641 tccgccggtg cgaaccacgg atgatctcgc ggcggtgttc gacgcgttca gccggtatga
     2701 cgtgaatcac ttgccggtgt cgatggcgga cagccgggat aaggtgatcg gcatgatcag
     2761 ccgatcggtg gtgattcgga agtatcagga agcgctgaag gggacatgaa ggcgaaatcc
     2821 gaatggcgaa gtccgaatcc ccgaatgaat gacgaatgac gaaatccgaa tgaagagggc
     2881 g
//
LOCUS       DATIMH010000929         1982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4262912, whole genome shotgun sequence.
ACCESSION   DATIMH010000929 DATIMH010000000
VERSION     DATIMH010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4262912"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VLI90_16120"
     CDS             <1..429
                     /locus_tag="VLI90_16120"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV15789.1"
                     /translation="REAAKSGWRLVSPSNQFYNLDIKSGGSAPGRLFADTQKALISGT
                     VFNDANGNKIKDGSEINLSGWRVYLDLNKDAKFDSGDASVVSDSGGNWQFGDLSPGTY
                     VVRVVLQSGWSLTTPSTGSFSFSVGFGSSRIGNRIGVKRT"
     gene            798..>1982
                     /locus_tag="VLI90_16125"
     CDS             798..>1982
                     /locus_tag="VLI90_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15790.1"
                     /translation="MTNAGDGLTTLREAIVAANSHAGDDSISFSPTVFTSGSLHTIKL
                     TGGQLTLSDTSGATTITGPGFAIAAVDGDGKSRVFQISSGVSASISGLTITGGLVDDS
                     AVDGAEALGGGIFNAGTLSLTGVTVTANSVIGGGGAAARGGGIYSAAGSLTISGSTIA
                     NNQSHGGLGSTVEGNCGSGGAGFGGGIYSAGALSVTDTGITSNAVSGGVNSQHQDKPA
                     DAFGGGAYVAGPASFIGSTISGNSATGANQDYLGQGTGNALGGGIYAGGASLTITQTI
                     VSSNQITGGNGGAETGPGGDALGGGVYTKATQTTISDSTISGNQASAGSGGEDQRPTY
                     EGTGAGGGVYSSTGSLTINTSTISGNSAGGGGGGIYASAGGLGAGGGVHSGGALTISR
                     STI"
BASE COUNT          364 a          624 c          658 g          336 t
ORIGIN      
        1 cgcgaagccg ccaagtccgg ctggcggctg gtctcgccgt ccaatcagtt ctacaacctc
       61 gacatcaaat ccggcggcag cgcccccggc cggttgttcg ctgacacaca aaaggcgctg
      121 atcagcggca ccgtgttcaa cgatgccaac ggcaacaaga tcaaggacgg cagcgaaatc
      181 aacctctccg gctggcgcgt ctatctcgac ctcaacaagg acgcgaagtt cgatagcggc
      241 gacgccagcg tcgtgagcga ctccggcggc aactggcagt tcggcgatct ctcgccgggc
      301 acctacgtcg tccgcgtggt cttgcaaagc gggtggtcgc tgacgacgcc gagcactggc
      361 agcttctcgt tctccgtcgg cttcggctcc agccgcatcg gcaatcggat aggcgtgaag
      421 cgcacttgag gcgttgaaaa gcgccgcccc cgaccacgcc gggttcggag gtactccgac
      481 gacccggatt ctgccgcgcg tcgagcagga gaatctggat cgcgagtacg cttccagccc
      541 ggcgcgggat acgcgcgatc gtgctcgcgt ttaaatgcgc tcatgcgatt tctttagcca
      601 cgcttggttt ttcgtgatac gctaagtagg tcactcggtc catccatttc tgctgagcta
      661 tgcgaaagcg agttcatatg cgcacgcgcg gagcctctcg tcattttcgt tgtatttcta
      721 acttttggca tacacagatc gaagcggttg agccacggcg gctgctgtcg gcgtttgcgg
      781 tcaacacgac gtccgacgtg accaacgctg gcgacggctt gacgacgctg cgcgaagcca
      841 tcgtggcagc caactcgcac gcgggagacg actcgatctc gttcagcccg accgtcttca
      901 ccagcggcag cctgcacacg atcaagctga ccggcgggca gctgacgctg agcgacacca
      961 gcggcgcgac gacgatcact gggccgggtt tcgctatcgc ggcggtggat ggtgatggca
     1021 agagccgcgt gtttcagatc agctccggcg tgagcgcgtc gatcagcgga ttgacgatca
     1081 ccggcgggct tgtggacgac agcgccgtcg acggcgctga ggcgctgggc ggcggcatct
     1141 tcaatgccgg cacgctctcg ctcacaggcg tcaccgtcac ggccaactcg gtcatcggtg
     1201 gcggtggcgc ggcggcgcgc ggcgggggca tctacagtgc cgcgggatcg ctgacgatct
     1261 ccggcagcac catcgccaac aaccaatcgc acggcggcct tggttccacc gtcgaaggga
     1321 actgcggcag cggtggcgcg ggcttcggtg gcggaatcta cagcgctggc gcgctcagcg
     1381 tcaccgatac cggcattaca tcgaacgctg tgtctggcgg cgtgaacagt cagcaccagg
     1441 acaaacccgc cgacgccttc ggcggcggtg cctatgtcgc tgggccggcg tcgttcattg
     1501 gcagcacgat cagtggcaac agcgccaccg gcgcgaacca ggactacctt ggtcagggaa
     1561 ccggcaatgc attgggtggc ggcatttatg ccgggggggc ttcgctgacg atcacgcaaa
     1621 cgatcgtctc gagcaaccag atcaccggcg gcaatggtgg cgcggagacc ggccctggtg
     1681 gcgacgcgct gggtggcggg gtctacacca aggcgacgca gacgacgatc tccgacagca
     1741 cgatcagcgg gaaccaggcc tcggccggaa gcggcggtga agatcagcgc ccgacttacg
     1801 aaggcaccgg cgcgggcggt ggcgtttaca gcagcacggg cagcctgacg atcaatacct
     1861 cgacgatctc cggcaacagc gccggcggtg gtggcggtgg gatctatgcc tcagccggtg
     1921 gattgggcgc tggcggaggc gtccacagcg gcggagcgtt gacgatctcg cgcagcacaa
     1981 tc
//
LOCUS       DATIMH010000930         4608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1958725, whole genome shotgun sequence.
ACCESSION   DATIMH010000930 DATIMH010000000
VERSION     DATIMH010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958725"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..2018
                     /locus_tag="VLI90_16130"
     CDS             39..2018
                     /locus_tag="VLI90_16130"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV15791.1"
                     /translation="MSSPSIPQLLQLATDHHRAGRLREAEPIYRQILAIDPQHADTLH
                     GLAFLVKQSRRLGEALALVQRAIGANAKRPAYWNSLGLILIDAGRIDEAADGLQKALA
                     LDPNCTDALINLGGIHAMRRQWAQAAALYRRVLVVRPGSPEALTNLGNVLQEMGKRDE
                     AIVVYRQTIALRPRFAQAHDNLGTVLEHAGQLEEAAASYRQAVALQPDYANALNNLGH
                     VCAMLGQLDEALDSFRRATVADPTASRFHSNLLYWSHFHPAYGAVAILREHQQWNTRH
                     AEPLRRFARPFENDRSPRRRLRIGYVSPDFHNHTMSLFTTPVLANHDRDQFEVFCYAS
                     DAKPDDFTDRLRGLSDHWRNILPMTDDRAAEQMRHDRIDILVDLSLHMGRNRLLVFAR
                     KPAPLQVTWLGYPGTTGLWAMDYRLTDPHLDPPGDERDRLYVERSVRLPHAFWCYDPR
                     ALDREDAAEIAVSPPPVVASGSIMFGCLNNFAKVHERVLAVWSRVLAAVPGSRLLLLA
                     PPGSHRQRVADRLSGRVQFVDRAPRRKYLASHAEIDIALDTFPANGHTTSLDALYMGV
                     PAVTLLGETAIARGGLSVLSNVGLMDLVARSPDEYVEIARKLANDRARLAELRATLRD
                     RLRASPLMDWSGFVRGLEARYREMWIEWVRGTESK"
     gene            complement(2128..3120)
                     /locus_tag="VLI90_16135"
     CDS             complement(2128..3120)
                     /locus_tag="VLI90_16135"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSV15792.1"
                     /translation="MIRSIVLLRCVLVLLPMALCACGKSEESTTSAARGTPVTLTLDW
                     KPEPEFGGFYAANDSGTFNKNGLDVMIKSAGAGAPTWQLVATGQTDFATTAADQVLIA
                     RARGADVVALFAVYQTAPNGIMVHEARNFTKIDDVFTHPGTLAAEDATWLRFCLTKFP
                     KPDVKIIGYSGGIAAFMAKPDYSQQCFVTSEPILAGKQRSDPQTFLVADLGYNPYTTV
                     VIAKSETVKNKAALVKSMITACRDGWRAYLNDPTPANNAMIRLNSEMDPGTFSAAADA
                     QKPLIETAETKAANLGIMTADRWNTLAKQLVDLKVIENTDVAKDCFVNPETLGK"
     gene            complement(3283..4083)
                     /locus_tag="VLI90_16140"
     CDS             complement(3283..4083)
                     /locus_tag="VLI90_16140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV15793.1"
                     /translation="MISRAVRTFVTRILPPVLVFVCAAVALELYVRIRQLPMYYMPRP
                     TDVLRIMLDAEQRRRLLASLLVTTEAALIGFTASVIVGILIAIALAASRWVRRAFYPY
                     TLFFQTVPIVAIAPLLVIWFQAGLTSVSVSAFIVSVFPVIANTLAGLLATEPALLDLF
                     QLYGAGSIERLWKLRLPYAMPSIVTGLRVAAGLAVIGTVVGEFLVGTLGESEGLGVRI
                     VSAMKNGRTDLVFAAVLIASLLGLALFAVVNLAGHLMLRRWHTSEQGA"
     gene            complement(4085..>4608)
                     /locus_tag="VLI90_16145"
     CDS             complement(4085..>4608)
                     /locus_tag="VLI90_16145"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV15794.1"
                     /translation="VFQDAHLLPWRNVLRNVELPLELMGVARSERTRRAMQVLDQVGL
                     TDALRRYPAQLSGGMRMRVSLARALVTDPRLLLLDEPFAALDEITRQRLDEQLRQLWI
                     VRGMTVVFVTHSTAEAVFLADRALVMTKRPGRIILDDPIDLPRERIGPLRATLEFATQ
                     TRAIYEALERGGA"
BASE COUNT          831 a         1401 c         1557 g          819 t
ORIGIN      
        1 cttcggcacg ggcgagacgc ccatgctaca actaactcat gtcttcgccg tccattccgc
       61 agcttcttca actggccacc gatcatcatc gcgccggacg gttgcgcgag gcggagccga
      121 tctatcggca gatcctggcg atcgacccgc agcacgccga tacgctgcat gggttggcgt
      181 ttctcgtaaa gcaatcgcgg cgattgggtg aagcgctggc gctcgtgcag cgcgccatcg
      241 gtgccaatgc caagcgcccg gcgtactgga acagccttgg cttgatcctg atcgacgcgg
      301 ggcggatcga tgaggcggcc gacgggttgc agaaggcgct cgcgttggac ccgaattgca
      361 ccgacgcctt gattaacctc ggcggcattc acgcgatgcg ccggcagtgg gcccaagccg
      421 cggcgctata tcggcgggtg ctggtcgtgc gaccgggatc gccggaggca ttgacgaacc
      481 taggcaacgt gctgcaggag atgggcaagc gcgacgaggc gatcgtcgtc tatcgccaga
      541 cgatcgcgct gcggccgcgg tttgcacagg cgcacgacaa cctcggcacg gtgctggaac
      601 acgcgggcca gctggaagag gcggcggcaa gttatcggca ggctgtcgcg ctacagccgg
      661 attatgcgaa cgcgctgaac aacctagggc acgtctgcgc gatgctgggt cagttggacg
      721 aggcgctcga ttcatttcgg cgcgcgacgg tcgccgatcc gacggcgagc cggtttcaca
      781 gcaacctgct gtactggagt cattttcatc cggcgtacgg cgccgtcgcg atcctgcgcg
      841 agcaccagca atggaatacg cggcacgccg aaccgctgcg gcgattcgcg cgaccgttcg
      901 agaacgatcg atcaccccgg cggcggttgc gcatcgggta cgtctcgccg gattttcaca
      961 accacacgat gtcgctgttc acaacgccgg tgctggcgaa tcatgatcgc gatcagtttg
     1021 aggttttctg ttacgccagc gacgccaaac cggatgattt tactgaccgc ctgcgtgggc
     1081 tgtcagacca ttggcgcaac atcctgccga tgaccgacga tcgcgcggcg gagcagatgc
     1141 gccacgaccg catcgacatc ctcgtggact taagcctgca catgggccga aatcggctgc
     1201 tggtcttcgc gcgcaagccc gcgcccctgc aggtgacgtg gctgggttac ccgggcacga
     1261 caggattgtg ggcgatggat tatcgcctga cggacccgca cctggatccg ccgggggatg
     1321 agcgcgatcg gctttatgtt gagcgctcgg tgcggctgcc gcacgcgttc tggtgttacg
     1381 acccgcgggc gctggaccgt gaagatgcgg cggagattgc ggtgtcgccg ccgccggtgg
     1441 ttgcgagtgg gagcatcatg ttcggatgcc tgaataattt cgcgaaggtg cacgagcgtg
     1501 tgctagcggt gtggtcgcgc gtgttggcgg cggtgccagg gtcgcggctg ctgctgctcg
     1561 cgccgccggg ttcacatcgg cagcgcgtcg ccgatcgttt gagtgggcgc gtgcagttcg
     1621 tcgatcgcgc gccgcgccgg aaatacctgg cgagtcatgc cgagatcgac atcgcgctcg
     1681 atacgttccc cgccaacggt cacacgacga gcctggacgc gctgtacatg ggcgtgccgg
     1741 cggtgacgct gttgggcgag accgccatcg cccgcggggg cttgagcgta ctgtcgaacg
     1801 tggggctgat ggacctggtg gcgcgatcgc cggatgaata cgtcgagatc gcgaggaagt
     1861 tggcgaacga tcgcgcgcgg ctggcggagc tgcgcgcgac gctgcgcgac cggttgcgcg
     1921 cgtcgccgct gatggactgg agcggttttg tgcgcgggtt ggaagcgcgg taccgcgaga
     1981 tgtggatcga atgggtgcgc ggcactgaat caaaatgacg ccccacgccg ggttcggagg
     2041 tactccgacg accccgattc ttgtcgcgcg tggagcaaag catccgggac gtcggagtac
     2101 ctccgaaccc ggcatgacat cggccactca cttccccagc gtctcgggat tcacaaaaca
     2161 atccttcgcc acatccgtat tctcgatcac cttcaggtcc accagctgtt tcgccagcgt
     2221 gttccagcga tcggccgtca tgatgccgag gtttgccgcc ttggtctccg cggtttcgat
     2281 gagcggtttc tgtgcatcgg cggcggcgga gaaggtgccg gggtccatct cgctattgag
     2341 gcggatcatc gcgttgttcg ccggcgtggg gtcgttcagg tatgcgcgcc agccgtcgcg
     2401 gcaggcggtg atcatcgact tcaccagcgc ggctttgttc ttcaccgttt ccgatttggc
     2461 gatgacgacg gtggtgtacg ggttgtatcc gaggtcggcg acgaggaacg tctgcgggtc
     2521 gctccgctgc ttgccggcga ggatcggctc ggatgtgacg aagcactgct gcgagtaatc
     2581 cggcttggcc ataaacgccg cgatgccgcc gctgtaaccg atgatcttca cgtcaggctt
     2641 ggggaacttg gtcaggcaga accgcagcca ggtggcgtcc tcggcggcca gcgtgccggg
     2701 atgggtgaag acgtcgtcga tcttcgtgaa gttgcgcgct tcgtgaacca tgattccgtt
     2761 gggcgcggtt tgatagacgg cgaagagcgc gacgacgtcg gcgccgcggg cgcgggcgat
     2821 cagcacctga tcggcggcgg tggtggcgaa gtcggtctga ccggtggcga ccagctgcca
     2881 ggtcggcgcg ccggcgccgg cggacttgat catcacgtcg aggccgtttt tgttgaacgt
     2941 gccagagtcg ttggcggcgt agaagccgcc gaactcgggc tccggtttcc agtccagcgt
     3001 gagcgtgact ggcgtgccgc gggcggcgct ggtagtggat tcttcgctct tgccgcaggc
     3061 gcacagcgcc atcggcagca ggaccagcac gcaacgcaga aggacgatcg atcggatcat
     3121 gtctcgctcc caaagatctc ttaaatgctc attcaccacc gaagcactct aaaaaagacg
     3181 gacgaagagg ttgaaccaca gatgcaaacc gatgaacaca gatgagaaaa tgagctgcat
     3241 gatctgtgtg catctgtggt tcctcatcct tgatctttac cctcaagcac cctgttcaga
     3301 tgtgtgccac cgccgcagca tcaggtgccc ggcgaggttg acgacggcga acagcgccag
     3361 gcccagcagc gacgcgatca gcacggccgc gaagaccagg tcggttcgcc cgttcttcat
     3421 cgcgctgacg atgcgcacgc ccaggccttc gctttcgccc agcgtgccga ccaggaattc
     3481 accgacgacc gttccgatca ccgcgagccc cgcggcgacg cgcaggccgg tgacgatgct
     3541 gggcatcgca tacggcaacc gcagcttcca caatcgttcg atcgatccgg cgccgtacag
     3601 ctggaacagg tcgagcagcg ccggttcggt tgccaacagt ccggcgagcg tgttggcgat
     3661 gaccgggaaa accgacacga tgaacgccga caccgacacg ctggtcaagc cggcctggaa
     3721 ccagatgacc agcagcgggg cgatcgcgac gatcggcacg gtttggaaaa acaacgtgta
     3781 cggataaaac gcccggcgca cccagcgcga cgccgccagt gcgatggcga tgaggatacc
     3841 gacaatcacg ctggcggtga aaccaatgag cgcggcttcg gtggtgacca gcagcgacgc
     3901 gagcaaccgc cggcgctgct cggcgtccag catgatgcgc agcacgtcag tgggccgcgg
     3961 catgtaatac atcggcagtt ggcggatgcg cacgtacaat tcgagcgcga ccgcagcgca
     4021 aacaaacacc agcacggggg ggaggatccg cgtgacgaag gttcggacgg cgcgcgagat
     4081 cacgttaagc gcctccgcgt tcgagggctt cgtaaatcgc ccgcgtttga gtggcgaatt
     4141 ccaatgttgc ccgtagcggc ccaattcgct cgcgcggcag atcgattgga tcatcgagga
     4201 tgatccgacc ggggcgcttg gtcatgacca gcgcgcgatc ggcgaggaag acggcttcgg
     4261 ccgtggagtg cgtgacgaag acgacggtca tcccgcggac gatccacagt tgccgcagtt
     4321 gttcgtcgag gcgctggcgc gtgatctcgt ccagcgcggc gaagggctca tcgagcagca
     4381 gcagtcgcgg gtccgtcacc aatgcgcggg cgagcgacac gcgcatgcgc attccgccgg
     4441 aaagctgggc gggatatcgg cgaagcgcat cggtcaatcc cacctggtcg agcacttgca
     4501 tcgcgcgccg cgttcgctcc gacctagcaa cgcccatcag ctccagcggc agctcgacgt
     4561 tccgcagcac gttccgccac ggcagcaggt gggcatcttg aaagacgt
//
LOCUS       DATIMH010000931        11788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4845601, whole genome shotgun sequence.
ACCESSION   DATIMH010000931 DATIMH010000000
VERSION     DATIMH010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 11788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11788
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4845601"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VLI90_16150"
     CDS             complement(<1..484)
                     /locus_tag="VLI90_16150"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HSV15795.1"
                     /translation="MNRRPIAALPVAMLENYFHLTARRSTVAREFRGAAATFLTMAYI
                     LFVNPSILKNAGLDFNSAVACTAAAAGVCCLLMGFIANFPLATAPGMGLNAVVAFQLA
                     KAAGSWQAAMGVIVLDGIVMLLLVLAGLREAIMDAIPRALRLATGAGIGLFIAFIGLV
                     N"
     gene            complement(481..789)
                     /locus_tag="VLI90_16155"
     CDS             complement(481..789)
                     /locus_tag="VLI90_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15796.1"
                     /translation="MLGNRRPIVTTTFVLLECGNAASRKPYRSSVARFREQMQAGNRI
                     VVPTEMDWQNAWTAYARGGPSAAGIVDQVSFIVMRRLRITEVFTNDRHFRTAGFNTLF
                     "
     gene            complement(878..1192)
                     /locus_tag="VLI90_16160"
     CDS             complement(878..1192)
                     /locus_tag="VLI90_16160"
                     /inference="COORDINATES: protein motif:HMM:NF014059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HSV15797.1"
                     /translation="MNGGIKRQDGRNAKGAEVEFVWSAFAMIESHQSGAAMTVRAIFE
                     NGVFRPTEPVDLPEKSEVEVILPASKPEGDANLDSIYKILGESYASGETDVAERHNEH
                     QP"
     gene            1213..2805
                     /locus_tag="VLI90_16165"
     CDS             1213..2805
                     /locus_tag="VLI90_16165"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HSV15798.1"
                     /translation="MNAAELWNRYKTHLCRVDSVGLTLDISRMKFDDAWLAGMEAAMQ
                     RAYAAMDALEKGAIVNPDEKRMVGHYWLRAPQLAPNTVITKEIESNIAAIKTFAADVH
                     AGRIKPPTAAHFTRVLSIGIGGSALGPEFVADALGDPTGDRMYVHFIDNTDPDGIERV
                     LKSIEGELAETLVIVETKSGGTPETRNGMLVVADAYKKAGLDFAKHAVAVTMFGSKMD
                     EIATSQGWLARFPMFDWIGGRTSELSAVGLLPAALQGIDIDAILTGAAAMDAATRVHE
                     TPKNPAALLALMWHHATGGKGQKDMVVLPYKDRLLLFSRYLQQLVMESLGKRLDLDGN
                     RVDQGINVFGNKGSTDQHAYVQQLRDGVNNFFVTFIRVLEDGGGEAGALQVEPGATSG
                     DFLHGFLLGTREALFGNDRESITITVNTVNARTIGVLIALFERAVGFYASLVNINAYH
                     QPGVEAGKKAAAAILELQAKVLAALTPEAQTAEQVAQKIGQPDAAETVFLLLEHLSAN
                     GRANAAGGSAGDPASAAYQASK"
     gene            complement(2790..4133)
                     /locus_tag="VLI90_16170"
     CDS             complement(2790..4133)
                     /locus_tag="VLI90_16170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HSV15799.1"
                     /translation="MGLSAMAARLAKAFLILLAVGLLAFNFVRIIQKPIGDFHNHWLL
                     GYNLLHGHSLYLGRGAQTPAERNPYPPFWALAHAPLTVVSAHQAQIIGFLPMYLGALG
                     LLLWTLTRLTRPHFPIVNSAARFWILAIPMLVALRFLSRDLSECGANIGLVALAWFAI
                     YSWTRDRDWLAGASLGLAIALKMTAGLFLPYFLWKRQWKMALATIAFAAAFSLSPVLV
                     RGPTRFANDLREWSAVVRAGHTGNPLIGVLGAATTNNFSLRPALGRLLMKSPTDPENP
                     VSPKLLDLPPRAASIIVDAIELSLLIAFAWIVRRPVTDRHDLTILYECAAVSLLILLL
                     SPITWGHHCVGTLPALVLIFLTGLSYRGLPRWMLVLVAYILLTLLLNRAFIGKDLSLL
                     VSNFSPVTWEIVCLLALTLGCGALVLSAPRAVDIHRQANPAASDPMPHPQHHFEA"
     gene            complement(4449..5345)
                     /gene="thyX"
                     /locus_tag="VLI90_16175"
     CDS             complement(4449..5345)
                     /gene="thyX"
                     /locus_tag="VLI90_16175"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HSV15800.1"
                     /translation="MSRLPAEIEKVQIERKPFEGEMHDMMAGAGRWEVKVHNHGLVAL
                     LDVMPRLAPAGKTADFAIVQAARVSYGAGTKQVNEDRGLIRYLARHRHTTPFEMVEFK
                     FHHVMPIFIARQWIRHRTANVNEYSARYSVVQDRFYRPSADNVRKQSASNRQGGEESI
                     DPMTADEFLKHLDEVEGAYEKYQKQIERGVSRELARIALPVSVYTEWYWKCDLHNVFH
                     FLSLRMDPHAQQEIRDYANAMFALIQPIVPIAAEAFLDYNFESLHLSRLEVEAIRSGQ
                     PLASENKRENAEWEEKRKKLGL"
     gene            5437..6108
                     /locus_tag="VLI90_16180"
     CDS             5437..6108
                     /locus_tag="VLI90_16180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HSV15801.1"
                     /translation="MVTVLENDQPHLETHAEERASSITHGAGIVFSIAAMIALLTVAD
                     VHRDARRIVTAAVYGFTLLLMYTASTCYHCVRSPRAKHAMRVFDHASIYLLIAGTYTP
                     IVLVAVGGTWGWSMFGVVWIIAVAGVILKMFFVDRYEPLSVTLYLATGWLALIAIKPM
                     IAALPAGAMAWIFGGGVAYTAGVVFFLWDHLPFNHAIWHLFVIAGSVCHFIAVLLYVL
                     PVHPV"
     gene            6155..7444
                     /locus_tag="VLI90_16185"
     CDS             6155..7444
                     /locus_tag="VLI90_16185"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HSV15802.1"
                     /translation="MQVLLLTIGSAGDVHPFVGIGLALRQRGHRVKVLTNPHFQPLIE
                     RAGLEFVAVGTADDYDQVRTNPDLWHPRKGFHTVFNIIINGFRPAFDAVMNNYVPGQT
                     VIAASSLGLAARCAQDKYNLPMATVHLQPAIFRSAIHPPKLPGMPIANWMPAGMKRFM
                     FTVADRMVINPLVAPPLNRFRGELGLAPVRDPLKDWWHSPQRVIGLFPQWYAPPQADW
                     PPQTRLTGFPRYDEGGISDMPAELAEFLTAGDPPIVFTPGSAMHHGEDFFRESVAACV
                     QLNRRGVLMSRHADHIPSNLPANVRHFAYAPFSQLLPKAAVCVHHGGIGTAAQAMAAG
                     VKQIVMPLAHDQFDNADRMQRLGIARSIPAKQYRAARVAKIVQELLADEAAAARCAEI
                     ARKFIGIEPLAQTCDLIEELPTARAAQGVGTSAALPA"
     gene            7813..8262
                     /locus_tag="VLI90_16190"
     CDS             7813..8262
                     /locus_tag="VLI90_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15803.1"
                     /translation="MNGLREFFNSTAGKAVAVFLILAGLVAIWISVRKNLGQSEGAAM
                     SRQRMFMCSETNKPFAHEISEGESYPVISPFTGKATGYPAEECWWTKDGQVKNEPTYV
                     LLKEATHQKGPTFCPDCGRLVVGHNPRAQAGEKPPPTKEEYTTRGSH"
     gene            8307..9368
                     /locus_tag="VLI90_16195"
     CDS             8307..9368
                     /locus_tag="VLI90_16195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV15804.1"
                     /translation="MRKQHVARALKGFTLVELLVVIGIIALLISILLPALGKARRSAQ
                     TLQCLANLRSMAQAMIMYTSENKSYIPGSALTTGRGIFDSTWTTQLVTSGNIPSESPI
                     QFTDYIMPLSKMMKLTLKTRNDPNEANRYREYMSLQQFLCPAFRDFLAGPFTGSPTNC
                     GSVQCISYVTATAFLVADGSLQGAPRGLARVSNAATWPQLPAGYTAKITKIQNSSYKI
                     YAADGGKFSQGTVPDYNLSINPAFSSLTYGDTQNFSDYGAWTKSTSAFDRSFAEGVNV
                     LDSRVFAYRHGTPGANLRPGAYKLNAVFYDGHAETLDEMQSCDPKYWAPTGSSLTDGT
                     RMWPDVVAKYGINSKYHVP"
     gene            complement(9398..10177)
                     /locus_tag="VLI90_16200"
     CDS             complement(9398..10177)
                     /locus_tag="VLI90_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15805.1"
                     /translation="MFTLDTSSFPTTTDELAQAVTVSLRKWVDRPMVAATGVYPSLDS
                     AEINLSGATFDAAEAPPDVRAVDQPTPGPTVDRFVLLAKPIQAHGVAAQLELTATKVR
                     LSYAHNRAGQTIVLLTDAAAGRLQIDIAQRDLENAALFAAQAAARPHGIAVQRVELSL
                     ACATPRELAVSARVTAKKLVTAIIRLSGRAIIDDQLNATLRNLAVDGDGMVAKIVVSL
                     IRPHLQKLEGQQQSLLALPGNIQLSGLALDASDGLKLTAHF"
     gene            10342..11781
                     /gene="purB"
                     /locus_tag="VLI90_16205"
     CDS             10342..11781
                     /gene="purB"
                     /locus_tag="VLI90_16205"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HSV15806.1"
                     /translation="MSEKSHDTYESPLAARNASREMLRLFSPQHKFSLWRRLWLELAR
                     AERELGITRITPEALSQMEAKLDEIDFARAADWEKRLRHDVMAHVHTFEEAAPAAKGV
                     IHLGATSQYVVDNADLMIMRDALGLVAVRLANAIDALGTFAEKWKDLPTLGYTHFQPA
                     QPTTVGKRATLWAQDLVIDLEEIEHRIATLRFRGVKGTTGTQASFLALFDGDHSKCDE
                     LDRRVTERFGFAESFAVTGQTYPRKVDAQVVSALAGVAASVHRFANDIRLLAGMKQVE
                     EPFEEEQVGSSAMAYKRNPMRCERATGLARFVISLASSPLQTAAEQWFERTLDDSSNK
                     RLAVPEPFLAIDGCLQILVNVARGLVVYPKTIEAALMAELPFMATEEILMAAVRGGGD
                     RQDLHEKIRRHSVAAAEIVKNFAQPNDLLDRLAKDPAFSAVNLSQVMDPKAFVGRAPE
                     QVTSFIASVVSPIRNRYAREIGQDVQLRV"
BASE COUNT         2300 a         3592 c         3734 g         2162 t
ORIGIN      
        1 cgttcaccag gccgatgaac gcgatgaaca gcccaatgcc cgcgccggtg gcgaggcgca
       61 gcgcgcgggg gatcgcatcc atgatcgctt cgcgcaatcc cgcgagcacc agcaagagca
      121 tcacgatgcc gtccaggacg atgaccccca tcgccgcctg ccacgacccc gcggccttgg
      181 ccagctgaaa cgcgacgacc gcgttcaatc ccatccccgg cgccgtcgcc agcggaaagt
      241 tggcgatgaa gcccatgagc agacagcaaa cccccgccgc cgccgccgtg cacgcgacgg
      301 cggagttgaa gtcgagcccg gcgtttttca ggatgctggg attgacgaac aggatgtacg
      361 ccatcgtgag gaaggtggcg gccgccccgc gaaattcgcg ggcgacggtg ctgcggcggg
      421 cggtaaggtg aaaatagttt tccagcatgg cgacgggaag ggctgcgatg ggtcgccggt
      481 tcaaaacaat gtattgaatc ccgcagttcg gaaatgccga tcgttggtga acacctcggt
      541 aatccgcagc cttcgcatca cgatgaacga gacttgatcg acgatccccg ccgccgatgg
      601 gccaccgcgg gcatatgcgg tccaggcgtt ttgccagtcc atctccgtcg gcacgacgat
      661 ccgattgccg gcctgcatct gctcgcgaaa gcgcgcgacc gacgaacggt atggcttccg
      721 tgaagccgcg ttaccgcatt ccaacaggac gaatgtcgtt gtgacgatgg gacgccgatt
      781 gcccagcatg ttttgatacg cttgtctcgc cagagcgtgc cactgatctg aatatcccac
      841 tcggcgagca acccaacagt atccaaaaac acgggcgtca tggctgatgc tcgttgtgac
      901 gctcagccac gtcggtttca ccggacgcgt acgattcccc caagatctta tagatcgaat
      961 cgaggttggc atcgccctcg ggcttcgacg ccggaaggat gacctcgacc tccgacttct
     1021 caggaagatc aaccggctcc gtcggtcgga acacgccgtt ctcaaatatc gctctcacgg
     1081 tcatcgctgc tcctgattga tgcgattcta tcatagcaaa cgccgaccac acgaattcca
     1141 cctccgcccc cttggcgttg cggccgtctt ggcgcttaat gccgccattc aaatgtaaag
     1201 gaacgtgacg cgatgaacgc agctgaactt tggaaccgtt acaagacgca cctgtgccgg
     1261 gtggattcgg tcgggctgac gctggacatc agccggatga agttcgacga cgcctggctg
     1321 gccggaatgg aagcggcgat gcagcgggcg tacgcggcga tggatgcgct ggagaagggc
     1381 gcgattgtca atccggacga gaagcggatg gtcggtcact attggctgcg cgcgccgcaa
     1441 ctggcgccca ataccgtcat caccaaggag atcgaaagca acatcgccgc catcaaaacc
     1501 ttcgccgcgg acgtgcacgc ggggcggatc aaaccgccca cggcagcgca cttcacgcgc
     1561 gtgttgtcga tcggcatcgg cgggtcggcg ctggggccgg agttcgtcgc cgacgccctc
     1621 ggcgatccta ccggcgaccg gatgtacgtt catttcatcg acaacacgga ccccgatggc
     1681 atcgagcgcg tgttgaaatc gatcgaaggc gaactggccg agacgctggt gatcgtcgag
     1741 accaagagcg gcggcacgcc ggagacgcgc aacggcatgc tggttgtcgc cgatgcatat
     1801 aagaaggcgg ggctggattt cgccaagcac gcggtggctg tgacgatgtt cggctcgaag
     1861 atggacgaga tcgcgaccag ccagggctgg ctggcgcggt ttccgatgtt cgactggatc
     1921 ggcgggcgga cgagcgaact gtccgccgta ggtctgttgc cggcggcgct gcaggggatc
     1981 gacatcgacg cgatcctgac cggggcggcc gcgatggacg cggcgacgcg cgtgcacgag
     2041 acgccgaaaa atcccgcggc gctgctggcg ctgatgtggc atcacgccac cggcggcaag
     2101 ggacagaagg acatggtcgt actgccgtac aaagatcggc tgctgctgtt cagccgatac
     2161 ctgcagcagc tggtgatgga atcgcttggc aagcgccttg acctggatgg caaccgcgtt
     2221 gaccagggca tcaatgtgtt tggcaacaaa ggatccactg atcaacacgc ctacgtccag
     2281 cagctgcgcg atggcgtgaa caacttcttc gtcacgttca tccgtgtgct ggaagacggc
     2341 ggcggggaag ccggcgcatt gcaggtcgaa cccggcgcca ccagcggtga tttcctgcac
     2401 ggcttcctgc tcggtacgcg cgaagcgctg ttcggcaatg atcgcgaatc gatcaccatc
     2461 accgtcaaca cggtgaacgc gagaacgatc ggtgtgctga tcgcgctctt cgaacgcgcc
     2521 gtcggctttt acgcctcgct ggtcaacatc aacgcgtatc accagcccgg cgtggaagcc
     2581 ggcaagaaag ccgccgcggc gattttggaa ttgcaggcaa aggtgctggc ggcgctgacg
     2641 ccggaggcgc agacggcgga gcaggtcgcg caaaagatcg ggcaaccgga cgcggcggag
     2701 acggtgtttt tgctgctgga gcacctgagc gcgaatggcc gcgcgaacgc cgcgggcggc
     2761 tcggccggtg atccggcaag cgctgcttat caagcttcga agtgatgctg aggatgcggc
     2821 atcggatctg acgccgcggg attcgcttga cgatggatgt caaccgcgcg aggtgcgctc
     2881 agcaccaacg cgccgcagcc gagcgtcagc gccagcaggc agacaatttc ccacgtcacg
     2941 ggcgagaagt tgctgacgag cagcgacagg tctttgccga tgaacgcgcg gttcaggagc
     3001 agcgtcagca ggatgtacgc gaccagcacg agcatccatc gcggcaggcc gcggtaagac
     3061 aggccggtta ggaaaatcag gaccagcgcc ggcagcgtgc cgacgcagtg gtgtccccag
     3121 gtgatcggcg acagcagcag gatgagcagc gagaccgctg cgcattcgta gaggatcgtc
     3181 aggtcatggc gatcggtgac gggccggcgg acgatccacg cgaaggcgat cagcagtgat
     3241 agctcgatgg cgtcaacgat gatggacgcg gcgcgcgggg gcaggtcgag caactttggc
     3301 gagacgggat tttccgggtc cgtgggggac ttcatcaaca accgacccag ggccgggcga
     3361 agcgaaaaat tgttggtcgt cgccgcccct aggacaccga tgagcgggtt tccagtgtgg
     3421 ccggcgcgga cgactgcgga ccattcccgc aggtcattgg caaagcgcgt cgggccgcgc
     3481 accagcaccg gcgaaagcga gaaagccgcc gcgaaggcga ttgtcgccag cgccatcttc
     3541 cattgtcgct tccacaggaa atacgggagg aacagcccgg ccgtcatctt cagcgcgatc
     3601 gctaatccca agctggcgcc ggcgagccaa tcgcgatctc gagtccatga atagatagca
     3661 aaccacgcca gcgcaaccag tccaatgttc gcgccgcatt cggacaaatc gcgcgagaga
     3721 aaccgcagcg cgaccagcat tggaatcgcg agaatccaga atcgagccgc ggaattcacg
     3781 atcggaaagt gcggtcgcgt cagccgagtc agcgtccaca gcagcagccc cagcgccccg
     3841 agatacatcg gcaagaagcc gatgatctgc gcctggtgcg cggagacgac cgtcagcggc
     3901 gcgtgcgcca gcgcccagaa cggcggataa ggatttcgct cagcgggcgt ctgggcgccg
     3961 cggccgaggt acagcgagtg tccgtgcagc aggttgtagc cgagcaacca gtggttgtga
     4021 aaatcgccga tgggcttttg gatgatgcgg acgaagttga acgcgagcag gccgacggcc
     4081 aggaggatca ggaacgcctt ggcaagtctg gcggccattg cagataagcc catttgtgcg
     4141 atccctgccg tcattaccga atgcattcca cgaggggccg ccaggatcta aacttcaaac
     4201 gtcaaggtta ggcgctgcgt tgcttcaccg aggcagacga aacgaccccg ttgttattga
     4261 cggacgcgaa gttgttcgca ccagtgccgc caagatcctt ccgaaaaggt gttgtttgag
     4321 atgtccattc gcagtgacag cccaggccgg aaggcccggg gatcgatttg cgaatggtgc
     4381 ggatatctca gtcgatccct ggccttccgg cctgggctat tatcgaagac tgctgcggag
     4441 gaggcgagtc acagtcccag cttcttccgc ttctcttccc actcggcatt ttcgcgcttg
     4501 ttttcactcg ccaatggctg tccgctgcgg atcgcttcca cttccagccg cgacaggtgc
     4561 agcgattcga agttgtagtc caggaacgcc tcggcggcga tcggcacgat cggctggatg
     4621 agcgcgaaca tcgcgttggc gtaatcgcga atctcctgct gggcgtgcgg gtccatccgc
     4681 agcgacagga aatgaaatac gttgtgcagg tcacacttcc agtaccactc ggtgtagacg
     4741 ctgaccggca gcgcgatgcg tgccagctcg cgcgagacgc cccgctcgat ctgcttctgg
     4801 tatttctcgt acgccccttc gacttcgtcg aggtgcttga gaaattcgtc ggccgtcatc
     4861 ggatcgatgg attcttcgcc gccctggcgg ttgctggcgc tttgcttgcg cacgttgtcg
     4921 gcgctgggac gatagaagcg gtcctgcacg acggaatatc ttgcgctgta ttcatttaca
     4981 ttggccgtgc gatgccggat ccattgccgg gcgatgaaga tcggcatcac gtggtggaac
     5041 ttgaattcga ccatctcgaa cggagttgtg tggcgatggc gcgccagata gcgaatcagc
     5101 ccgcggtctt cgttcacctg cttggtgccg gcgccgtagc tgacccgcgc ggcttggacg
     5161 atggcgaagt cggctgtctt tccggcgggc gccaagcgcg gcatcacgtc cagcagcgcg
     5221 accaggccgt ggttgtgcac cttcacttcc cagcggccag cgccggccat catatcgtgc
     5281 atctcgcctt cgaatggctt gcgctcgatc tggactttct cgatctcggc gggcagacgg
     5341 ctcatagcga tgcaatatag ctttcatagc cgggggcgga agccccggtg tttttcatct
     5401 cacgccaacc gaccagggct tccgcccccg gctatcatgg tcacggtgct ggagaacgat
     5461 caaccgcatc ttgagacgca cgcggaagag cgcgccagtt cgatcaccca tggcgcgggc
     5521 atcgtattca gcatcgcagc gatgatcgcg ctcctcaccg tcgccgacgt tcatcgtgat
     5581 gctcgccgca tcgtcaccgc ggcggtttac ggcttcacgt tattactgat gtacaccgcc
     5641 agcacgtgtt accactgcgt tcgttcgccg cgggcgaagc atgcgatgcg ggtgttcgat
     5701 cacgcgtcga tttatttgct gatcgcgggg acgtacacgc ccatcgtgct ggtcgcagtg
     5761 ggcgggacgt gggggtggag catgttcggc gttgtgtgga tcatcgccgt cgcgggagtc
     5821 attttgaaga tgtttttcgt ggatcgatat gaaccgctga gcgtgacgct gtacctcgcg
     5881 acgggttggc tggcgctgat cgcgatcaaa ccgatgatcg cggcgttgcc ggcgggagcg
     5941 atggcgtgga tcttcggcgg cggcgttgcg tacaccgcgg gcgtcgtctt ttttctgtgg
     6001 gaccacctgc cgttcaatca cgcgatctgg catctgttcg tgatcgcggg cagcgtgtgc
     6061 catttcatcg cggtgctgct gtacgtgctg ccggttcacc ccgtataaat ccgcccggaa
     6121 aagaaaccgc cgccactggc ggcgactata cataatgcaa gtgcttcttc tgacgatcgg
     6181 cagtgctggg gacgtgcatc cctttgtggg gatcgggttg gccctgcgcc agcgcgggca
     6241 tcgggtgaag gtcctgacca acccgcactt tcagccgctc atcgagcgcg cgggcctgga
     6301 gttcgtcgcg gtggggacgg ccgatgatta cgaccaggtt cgaaccaatc ccgacctttg
     6361 gcatccgcgc aagggctttc acaccgtctt caacatcatc atcaacggct ttcggccggc
     6421 gtttgatgcg gtgatgaaca actacgtgcc gggccaaacg gtgattgcgg cgtcgagcct
     6481 gggattggcc gcgcgctgcg cgcaggacaa atacaacctg ccgatggcga cggtgcatct
     6541 gcagccggcg atctttcgca gcgcgatcca cccgccgaag ctgccgggga tgccaatcgc
     6601 caattggatg cccgcgggga tgaagcggtt catgttcacg gtggcggacc gcatggtgat
     6661 caacccgctg gtggcgccgc cgttgaatcg attccgtggc gagctgggtt tggcgccggt
     6721 tcgcgatccg ctcaaagact ggtggcattc cccgcagcgg gtgatcgggt tgttcccaca
     6781 gtggtacgcc ccgccgcagg cggattggcc gccgcagacg cgactgactg gtttcccgcg
     6841 gtacgatgaa ggcgggatta gcgatatgcc ggcggagttg gcggagttcc tgaccgcggg
     6901 cgatccgccg atcgtgttca cgccaggcag cgcgatgcac catggcgaag atttctttcg
     6961 cgagtccgtc gccgcgtgcg ttcagctgaa ccgccggggt gtgctgatgt cgcggcacgc
     7021 ggatcacatt ccatcaaacc tcccggcgaa cgtgcggcac ttcgcctacg cgccgttcag
     7081 ccaactgctg cccaaggcgg cggtgtgcgt gcaccacggc gggatcggca ctgccgccca
     7141 ggcgatggcc gcgggcgtga agcagattgt catgccgctg gcgcacgatc aattcgataa
     7201 cgccgatcgc atgcagcgcc tgggcatcgc gcgaagcatt ccggcgaagc agtatcgggc
     7261 ggcgcgggtg gcgaagatcg tgcaggaact gctcgccgac gaggcggccg cggcgcgatg
     7321 tgccgaaatc gcgcgaaaat tcatcggcat cgaaccgctc gcgcaaacat gtgacctgat
     7381 cgaagaactc cccaccgcgc gtgccgcgca aggcgtcgga accagcgccg cgctgccggc
     7441 ataagcgtgc aaatgctgga gttaacaagt taagccgaag aaatgcggtc ggaacccgtg
     7501 actaacggct ggcgaacgcc cgcttaacca acggccaatg tcgaaactct gagctcacgg
     7561 ttgccgtctg aatgggcacg gcaggctcaa aaaatcgcgt gtcgggcctc ggtatttctt
     7621 ttgctctgcg gaaaccggct agtgtatgat gttccccgcg caggagttca cgcagcgcta
     7681 acgcggcaca catcagcgag aacggatcgc gcatttacgg tcgtggtaat gacagtccag
     7741 acgagcaaca ggcgcaggtc aggcgaacat gcgcgccggt caggcaatgg tggcaacgcg
     7801 ggagcgctta gcgtgaatgg tcttcgagag ttttttaaca gcaccgctgg aaaagccgtt
     7861 gccgttttcc tcatcctcgc tggtctggtc gccatctgga tttccgttcg taagaatctt
     7921 ggacaatccg aaggcgcggc catgtcccgg caacggatgt tcatgtgctc agagacgaac
     7981 aagcccttcg ctcacgaaat ctccgaaggg gagtcctatc cggtgatatc gcccttcaca
     8041 ggcaaggcga cgggttatcc cgcggaggag tgctggtgga cgaaggatgg ccaggtcaag
     8101 aacgagccga cctacgtcct gctgaaggaa gccactcatc aaaaagggcc cacattctgc
     8161 ccggattgcg ggcgattggt cgtcggccac aacccacgtg cgcaggccgg cgagaagcca
     8221 ccgcccacaa aggaagaata cacaaccagg ggtagtcatt agacggttcg ggccgtctgg
     8281 gtttttgctt tgagaggagt cagatcatgc ggaaacagca cgtcgcgcgc gcgcttaagg
     8341 ggttcacctt ggtggaactt ctagtggtga tcggcatcat tgcgctgctc atctccatcc
     8401 tgctgcccgc gttgggcaaa gcacgccggt cggcccagac ccttcagtgc ctggctaatc
     8461 tgcgatcgat ggctcaggcc atgatcatgt acacctcgga gaacaagtcg tacatccccg
     8521 gctcggcctt gacgaccggt cgcgggatct tcgacagcac ctggaccacc cagctggtga
     8581 cctccggcaa cattccctcg gaaagcccga ttcagttcac cgactacatc atgccgcttt
     8641 cgaagatgat gaagttgacg ctgaagacgc gcaacgaccc gaacgaggcg aaccggtatc
     8701 gcgaatacat gtcgctgcaa cagttccttt gcccggcgtt tcgcgatttc cttgccgggc
     8761 cgttcacggg atccccaacc aactgcggaa gcgtgcaatg catcagctac gtgaccgcaa
     8821 ccgccttcct cgtcgccgac ggtagcctgc aaggcgcccc gcgcggtttg gcccgcgttt
     8881 ccaacgcagc aacctggcct cagttgcccg ccggatacac ggccaagatc accaagatcc
     8941 aaaacagctc ttacaagatc tatgcggccg acggcggtaa gttctcgcag ggaaccgtgc
     9001 cagactacaa cctgtcgatc aaccccgcct tctcgtcgtt gacctacggc gatacgcaaa
     9061 acttctccga ctacggcgcg tggaccaagt caacctccgc cttcgatcgc tcgttcgccg
     9121 aaggcgtgaa cgtgctcgac tcccgagtgt ttgcctatcg tcacggcacg cccggcgcga
     9181 accttaggcc gggcgcatat aagttgaacg cggtgttcta cgacgggcac gccgagacat
     9241 tggatgagat gcagtcgtgc gatccgaagt actgggcgcc caccggctcg tcgctgaccg
     9301 atgggacgcg catgtggccc gacgtcgtcg ccaagtacgg catcaactcc aagtaccacg
     9361 ttccctgatt ggcagttcac caatcgagtc cgtccgctca aaaatgggcg gtcaacttga
     9421 ggccgtcgct ggcatcaagg gcaagaccgc tcagttgaat attacctggc aaggccagca
     9481 gcgattgctg ctggccttcc agcttttgca ggtgcgggcg gatcaggctg accacgatct
     9541 ttgcgaccat gccgtcgccg tcgacggcca ggttccgcag cgttgcattc agctgatcgt
     9601 caatgatcgc ccggccgctc aggcggatga tggcggtgac cagcttcttc gccgtcacgc
     9661 gcgccgacac cgccagctcc cgcggcgtgg cgcacgccag cgacaactcc acccgctgca
     9721 ccgcgatgcc gtgcggccga gccgccgctt gagcggcgaa cagcgccgcg ttctccaggt
     9781 cgcgctgcgc gatgtcgatc tgcaaccgcc ccgccgcggc gtcggttagc agtacgatcg
     9841 tctgccccgc gcggttgtgc gcgtaagaca accgcacctt cgtcgccgtc agttccagtt
     9901 gagcggcgac gccatgggcc tggatcggct tggccaacaa gacaaatcgg tccaccgtcg
     9961 gtccgggggt cggctggtca accgcccgaa catccggcgg cgcctcagcc gcgtcgaacg
    10021 tcgcgccgct caagttgatc tcagccgaat ccagcgacgg ataaacgccg gtcgcggcaa
    10081 ccatcgggcg atcgacccac ttgcgaagcg acacggtcac cgcttgcgcg agttcgtcgg
    10141 tcgtggtcgg aaatgaactg gtgtcgagcg tgaacattcg aatgattcta ttgcaacgtc
    10201 agagcaatgc ggaacgtcct cgtttagccg ccgcggcctc gcggcgcgga cgtagaaaat
    10261 caccgcgagg ccgcggcggt taaacgcaga tgccgtgcgc gctaggaatc ttccccggcg
    10321 actggttaga atcccggcga aatgtctgaa aaatcccacg atacctacga atccccgctc
    10381 gccgcacgca acgcttcgcg cgagatgctg cgcctgtttt cgccgcagca taagttttcg
    10441 ctgtggcgcc ggctttggct ggagttggcc cgcgctgagc gagaactcgg catcacgcgc
    10501 atcacgcccg aggcgctgtc gcagatggag gcgaagctcg acgagatcga cttcgcccgg
    10561 gcggccgact gggaaaaacg gcttcgccac gacgtgatgg cccacgtgca cacgttcgaa
    10621 gaagccgccc ccgcggccaa gggggtcatt cacctgggtg cgaccagcca gtatgtcgtc
    10681 gataacgccg atttgatgat catgcgcgac gccctggggc tggtcgccgt gcgattggcc
    10741 aacgcgatcg acgcgctcgg cacgttcgct gagaaatgga aggacctgcc aacgctgggt
    10801 tacacgcatt tccagccggc ccagccgacc accgtcggca agcgcgcgac actgtgggcc
    10861 caggacctgg tgatcgacct ggaagagatc gagcaccgca tcgccacgct gcggttccgc
    10921 ggcgtcaaag gcaccaccgg cacgcaggcg tcgttcctgg cgctgttcga tggcgatcat
    10981 agcaaatgcg atgaactgga ccgccgggtg accgagcggt ttgggtttgc cgaatcgttc
    11041 gccgtgaccg ggcagacata tccgcggaag gtggatgcgc aggttgtgag cgcgctcgcc
    11101 ggcgttgccg cgagcgtgca tcggtttgcc aatgacattc gattgctcgc cgggatgaag
    11161 caggtcgagg agccgttcga ggaagagcag gtgggttcca gcgcgatggc ctacaagcgc
    11221 aacccgatgc gctgcgagcg ggcgacgggc ctggcccggt tcgtcatcag cctggccagc
    11281 tcgcccctgc agacggcggc cgagcagtgg ttcgagcgca cgctcgatga ttccagcaac
    11341 aagcggctag ccgtgcccga gccgttcctg gcgatcgacg ggtgcttgca gatcctggtc
    11401 aacgtcgccc gcgggttggt ggtctatccc aagacgatcg aagccgcgct gatggccgag
    11461 ctgccgttca tggcgaccga ggaaattctg atggccgccg tccgcggggg gggcgaccgg
    11521 caggatttgc atgaaaaaat acgccgccat tccgtcgcag cggccgaaat cgtaaaaaat
    11581 tttgctcaac cgaacgattt attggaccgg ctagcaaaag accccgcttt ttcagccgtg
    11641 aacttgtctc aagtgatgga cccaaaggcc tttgttggcc gggcacccga gcaggtcacc
    11701 tcatttatcg catccgtagt ttcgccgata cgcaatcgat atgctcgtga aattgggcag
    11761 gatgtacagc ttcgggtctg aagtagca
//
LOCUS       DATIMH010000932         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5709944, whole genome shotgun sequence.
ACCESSION   DATIMH010000932 DATIMH010000000
VERSION     DATIMH010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5709944"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..660
                     /locus_tag="VLI90_16210"
     CDS             28..660
                     /locus_tag="VLI90_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15807.1"
                     /translation="MLDPDGTAHQAMELVNRFSFGSGLAAPAAYANFFRGWIDAGNRL
                     DVVLEIHNVQSHECPHLSHAFIEGGVPRGTLAEAMNTAIVTRFKAANLLYAPHTQTND
                     WPARRFGGWLSEHYGPILIVYEINAQDPTRHLDLSELKGTGALFTEAAGDFLFSAAGT
                     ELSNVVDQVRAARERRRAGNPATQTARDALEFEEAVRSRANPQDTEPGGL"
     gene            complement(868..>2527)
                     /locus_tag="VLI90_16215"
     CDS             complement(868..>2527)
                     /locus_tag="VLI90_16215"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSV15808.1"
                     /translation="GTFAGSAPLVPFGKTATSPNAQGTHIAITGNASIAAYVFGADSQ
                     LGSSFTIAASSSAFDTTLVVFAYDSVFGSSNGTLVAYNDDFSGTNSQVSFTATAGVKY
                     IACVKPFNNAGGPYTLTANYGGAPSESTFSLNNAGDGSINTGTIPAFGEANTYKFLAP
                     TGILGGATISLTNQTGDGDIYVFDDAGNLLGGNNTGGTGVETITLASVTAGATYHITV
                     IPENYAAQLTQSTVSVDVVVIARPAAPSLDSGSDTGYLQTDGITKKNASLDFFVNGTP
                     GSFVRLFRDGVGVTSGTVGPFGLISLTDPGPLADGTYTYTATAAATSLGSQTSASSGT
                     SITIDTQAPALNGFTFIYTSGPNDHAFGLQFDASNITLQPSNISVKNLTTNVTLDSSQ
                     FNLDDQFAGLFAYTYNPTAGVDRLPSGNYELRIPAAAFADYAGNTNPLMLQTFFFQDG
                     DADHNGKVDVGDLGILATNYGLSSKAFSQGDFNYDTKVDVGDLGILATNYGVNISGGS
                     GASTAAMPAAGVGPGPFLQQTTSPLLAWLDPSARRTLVESVGLA"
BASE COUNT          421 a          771 c          871 g          464 t
ORIGIN      
        1 tagcggtgcc gattcctcat catcccgatg ctggaccccg acggaacggc gcatcaggcg
       61 atggagctgg tgaaccgctt ctcgttcggc tcgggtttgg ccgcgcccgc ggcgtacgcg
      121 aacttcttcc gcggctggat cgacgccggc aatcggctgg atgtcgtcct ggaaatccac
      181 aacgtgcagt cgcacgaatg tccgcacctg tcgcacgcgt tcatcgaagg cggcgtcccg
      241 cggggcacgc tggccgaggc gatgaacacc gccatcgtca cccgcttcaa ggccgccaat
      301 ctcctgtacg cgccccacac gcagacgaac gattggcccg cgcggcggtt tggcggctgg
      361 ttgagcgaac attacggacc gatcctgatc gtctacgaga tcaacgcgca ggatccgacg
      421 cgtcacctgg atctgtccga actgaagggc accggcgcgt tgttcaccga agccgccggc
      481 gattttctct tcagcgccgc cggcactgaa ttgtcgaacg tggtggacca agtccgcgcc
      541 gcccgggaac gccgccgcgc cggcaatccc gccacgcaaa ccgcacgcga tgcgctggaa
      601 tttgaagagg cagtgcgttc acgcgccaac ccacaagaca ccgaacctgg cgggctgtga
      661 cgcgcgcgac gcgcgcggat gacggccgcg gagatctaag attgtctcgt cactcaggag
      721 gcgccaccgt ttgccgagtg cgcggttgtc aaagcgcgtc cgggcccttc cgcctctccc
      781 atgtaatcgt gggagaggga cggggtgagg gtgatttgga gcaccagcgg ctaccggcat
      841 tcgaaatcgc cctcaccctg acccgaccta cgccaagccg accgattcca cgagcgttcg
      901 ccgggcggat ggatcgagcc acgccaagag cgggctggtt gtctgctgca ggaacgggcc
      961 cgggccgaca cccgccgcgg gcatggcggc ggtgctggcg ccgctgccgc cgctgatgtt
     1021 cacgccgtag tttgttgcga ggatgcccag gtcgccgaca tccaccttcg tgtcgtagtt
     1081 gaaatccccc tgcgagaaag ccttggagct caggccatag ttggtcgcga ggatgcccag
     1141 gtcgccgacg tcaaccttgc cgttgtgatc ggcgtcgccg tcctggaaaa agaaggtctg
     1201 caacatcagc ggattggtgt tcccggcgta atctgcgaac gcggcggcgg ggatgcgcag
     1261 ctcgtagttc ccgctgggca ggcgatcgac gccggccgtt gggttgtacg tgtatgcgaa
     1321 cagtccggcg aactgatcgt ccaggttgaa ttggctcgag tccagcgtca cgttcgtcgt
     1381 caggttcttc acgctgatgt tcgacggctg cagcgtgatg ttgctggcat cgaactgcaa
     1441 accgaacgcg tgatcgtttg gcccgctggt gtagatgaag gtgaagccgt tcaacgcggg
     1501 ggcctgggta tcgatggtga tcgacgtgcc ggacgacgcc gatgtctgac tgcccagcga
     1561 cgtggcggcg gcggtggccg tgtaggtgta cgtgccgtcc gccagcggac cggggtcggt
     1621 cagggaaatc agaccaaagg ggccgacggt accgctggta acgccgacac cgtcgcggaa
     1681 cagccgcacg aacgagccgg gcgttccatt gacgaagaag tcgaggctgg cgtttttctt
     1741 ggtgatgcca tcggtctgca ggtagccggt gtcggaaccg gagtcgagcg acggcgccgc
     1801 cggcctggcg atcaccacca catcgaccga tacggtcgat tgggtgagct gcgcggcgta
     1861 gttttccgga atgacggtga tgtgatacgt cgcgcccgcc gtcacgctgg ccagcgtgat
     1921 cgtctcgacg cccgtgccgc cggtgttgtt tccgccgagc aggttgccgg cgtcgtcgaa
     1981 gacgtagatg tcgccgtcgc cggtttgatt ggtcaggctg atcgtcgcgc cgccgaggat
     2041 gccggtcggc gcgaggaact tgtacgtgtt ggcctcgccg aaggcgggga tcgtgccggt
     2101 gttgatcgaa ccgtcgccgg cgttgttcag ggaaaacgtc gattccgacg gcgcgccgcc
     2161 gtagttcgcg gtgagcgtgt atgggccgcc ggcgttgttg aagggcttca cgcaggcgat
     2221 gtacttcacg cccgccgtgg cggtgaagct cacctggctg ttggtgccgc tgaagtcatc
     2281 gttgtacgcc accagtgtgc cgttggatga accgaagacc gagtcgtacg caaaaacgac
     2341 cagcgtcgtg tcgaacgcgc tgctgctggc ggcgatggta aacgagctac cgagctggct
     2401 gtccgcgccg aagacgtacg ccgcgatgct ggcgttgccg gtgatggcga tgtgcgtgcc
     2461 ctgcgcgttg gggctggtgg cggtcttgcc gaaggggacc agcggcgcgc tgcccgcgaa
     2521 tgtgccg
//
LOCUS       DATIMH010000933         1973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_800936, whole genome shotgun sequence.
ACCESSION   DATIMH010000933 DATIMH010000000
VERSION     DATIMH010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800936"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..1873
                     /locus_tag="VLI90_16220"
     CDS             359..1873
                     /locus_tag="VLI90_16220"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HSV15809.1"
                     /translation="MTRALSIRTAVLASIALLLSSQLAPAHAEKRVALVIGNNDYRNV
                     PKLQKAVNDARTMGETLKQLGFSVMIAENQTRQAFSQSLLAFDATIEKGDTAFFFFAG
                     HGFEIAGQNYLLPTDVPAATEGQEELVRDASILADRIIERLQNRQARTAILVFDACRN
                     NPFERTGTRAVAGGGGLAPMNTLPEGVFSVFSAGPRQTALDRLSENDNNPNSVFTRTF
                     AKELLQPGENMVQVAQRTRRVVSELADTVKHKQVPVYFDQMVDDVFLNGATGKSDVPV
                     ARPAEPPQKMAALPPVQTSPLPRNAPTDSVNAPIAMFSRHNGGWTVVFSIADPTLGIS
                     WRMGDSGDFRETGFIDTLDPRTRKRMPNPSIELPADAPAATIQVRYVDLSGETQGPFP
                     IRFDPQAALIRDQRKILDLTATSWLSFRDFNGLLVYYTQLMSYRCAIREARFGIDTTV
                     PDKVFKMPPCDPRDPSVIPRDAQPYVKLPPATKSVSVELTYQDGSVSEIKSFRR"
BASE COUNT          386 a          637 c          611 g          339 t
ORIGIN      
        1 tctcgaaaaa ctgtcgcgct aaaccccgga acggcacgca aatttggcac ggggaacgta
       61 gatacgcgag caggcggctt gttctaggta tcgcgcttcg ataccgggcc gcgtcacggc
      121 cggaacataa ggtccaaatg ccggaagtca atccggaacc ggccggaaac cccgaaaaag
      181 cttaatgttt ccgaacgatt gcatggctga tgggtaccga ccgggagcgc cgggcggccg
      241 gcatccggct gggtaaggca cgccggcaac aggcttgaac ccggcttggg ccattcacga
      301 ctacctcttg ttgcggtccg cacgggccgg ggcgagcggt ggacatgcgg gggatcggat
      361 gacgcgggcg ctgtcgatca ggactgcggt gctggcaagc atcgccttgt tgctttccag
      421 tcagcttgcg ccggcccacg ccgaaaagcg cgtcgcgctg gtgatcggca acaacgacta
      481 ccgcaatgtg cccaagctgc agaaggcggt gaacgacgcc cgcaccatgg gcgagaccct
      541 caagcaactc gggttttcgg taatgatcgc cgagaaccag acccgccagg ccttcagcca
      601 gagcctgttg gccttcgatg ccaccatcga aaaaggcgat accgcatttt tcttttttgc
      661 cggccacggt tttgagatcg ccgggcagaa ttatctgctg ccgaccgacg tgccggcggc
      721 gaccgagggc caggaagaac tggtgcgcga cgcctcgatc cttgccgacc ggatcatcga
      781 acggctgcag aaccggcagg cacgcacggc cattttggtg ttcgacgcct gccgcaacaa
      841 cccgtttgag cgtaccggca cccgcgcggt ggccggcggc ggcgggctcg cgccgatgaa
      901 cacgctgccc gaaggcgtgt tctcggtttt ctcggcgggg ccgaggcaga ccgcgctcga
      961 tcgtctttca gagaacgaca acaatcccaa ttcggtcttc acacggacct ttgccaagga
     1021 attgctgcag cccggcgaga acatggtcca ggtcgcgcag cgcacccggc gcgtcgtgag
     1081 cgaactggcc gacaccgtca agcacaagca ggtgccggtc tatttcgacc agatggtgga
     1141 tgacgtgttc ctcaacggcg ccaccggaaa gtcggatgtc ccggtggcca ggcccgccga
     1201 accgccgcag aaaatggcag cgttgccgcc ggtgcagacc agtccattgc cgcgcaacgc
     1261 gcccacggac tccgtcaatg cgccgatcgc aatgttctcc cgccacaacg gcggctggac
     1321 cgtggtgttc tcgattgccg acccgacgct ggggatttcc tggcggatgg gcgacagcgg
     1381 cgatttccgc gagaccggct tcatcgacac gctggatccg cgcacccgca agaggatgcc
     1441 caatccatcc atcgaactgc cggcggacgc gcccgccgcg accatccagg tccgctatgt
     1501 cgatctttcc ggcgaaaccc agggcccatt tccgatccgg ttcgatccgc aggcggcgct
     1561 gatccgggac cagcgcaaga tcctcgatct caccgcgacg agctggctgt cgttccgcga
     1621 tttcaacggc ttgctggtgt attacaccca gttgatgtcc taccgctgcg ccattcgcga
     1681 ggcccggttc ggcatcgaca ccaccgtgcc cgacaaggtg ttcaagatgc cgccctgcga
     1741 cccgcgcgat ccatccgtca tcccgcgcga cgcgcaaccc tatgtgaaac tgccgcctgc
     1801 aaccaagtcg gtgtcggtgg aattgaccta ccaggatggc agcgtctcgg agatcaagag
     1861 tttccgtcgc tagatactga tccgatggcc tgagagctcc cattcgcgag ggcgaagatg
     1921 gacggcgaag gttttccgca acgggccgag caactgcgca aagccgtgaa cgt
//
LOCUS       DATIMH010000934         6385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1668719, whole genome shotgun sequence.
ACCESSION   DATIMH010000934 DATIMH010000000
VERSION     DATIMH010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668719"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VLI90_16225"
     CDS             complement(<1..1188)
                     /locus_tag="VLI90_16225"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV15810.1"
                     /translation="MNLSEPFIRRPVMTVLLTVSAILFGVLAYKALPVNDLPAVDYPV
                     IQVSVAYPGATPETMANNVATPLERQFMQIQGLELVTSSSGQGNTNFTLQFSLDKSID
                     AAATDVSTAITQATGQLPPDLPSPPTLSKTNPNDQPIKYIAMFSNSVTRGQLYDYAST
                     QAGQRISLVPGVSRVQVYGTPSAVRIKADPTALAVRGLSIEDLSKAIQNGTSYQGAGQ
                     FDGVHKTFLLQPQGQLENAEAYNNLVIGFRNNAPIFLKDVATATDSVQDERIDLRFWV
                     RDRQVPTATVVIAVFRQAGANAVSVSKAVEDLIPTIQQTLPNAVQIHTIYDRSLSIVH
                     SIDDVQETLFIAFVLVVLVIFAFLGRATDTLIPAVALPLSLLLTFVAMQALGYSLDNL
                     SLMA"
     gene            complement(1319..2626)
                     /locus_tag="VLI90_16230"
     CDS             complement(1319..2626)
                     /locus_tag="VLI90_16230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSV15811.1"
                     /translation="MALLAAVSGCRRGDANAMSGPMPPPMVTVAVAEAKDVPVYLDEP
                     VGRAVATEAVTIQPQVTGMLMARHFEDGADVNKGCILFEIDKRPFEAGLAQAQATLLE
                     NQAAREFAQQDFARVEHLQGTSAVSQQEYDQKKNALAVAEAKVKAGEAAVQTAKLNLD
                     YCQIHSPIDGRAGHRLIDPGNVVWSSGPNGGTNMLTIQKIDPIYADFTITENDLTLVR
                     RHMAEGTLKVLADLPQDQPSPMNVAGSPPTTQAATTQPAVAEAMPQATTQPVAAQAAQ
                     HFEPREGQLVFLDNAVQDGTGTVKLRALIPNQDRHFWPGQFVHVKLVLTVKKDAVLIP
                     NQATQISQKGPYVYVIKHEEKDGQATDVAEFRPIVPGQRQGDMLVIEQGVAAGERVVL
                     TGQLMLQPDHPGPVTIAPPTGAGGPPGAAHADADQDHNSKKAS"
     gene            complement(2917..4278)
                     /locus_tag="VLI90_16235"
     CDS             complement(2917..4278)
                     /locus_tag="VLI90_16235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV15812.1"
                     /translation="MEQQTTAAGTAAGSDATANTLEYGQEGTIGRYRWVICALLFFAT
                     TVNYVDRSVLAVLAPTLKQKIGWTDTNYGDINAAFSAAYAIGLLAAGWFIDKFGTRMG
                     YTVALIFWSFASIAHAFARTAFGFGACRVVLGVAEAGNFPAAIKTVAEWFPKKERSFA
                     IGLFNSGSNIGAILAPVLVPWITITWGWQAAFIITGAAGLIWVAFWLPLYRVPAEHPR
                     LSKSELAYINSDAAEAVTPVPWSKLLPHRQVWAFSLAKALTDPIWWFWLFWAAPYLNK
                     RFGVDIKTIGIPLVIIYNFATVGSIGGGYLSAGLIRAGWSVNAGRKTALLICALLVLP
                     VTVLTKFNNEWVAVLLIGLAAAAHQGFSANLFALTGDLFPRRVVGSITGIGGMFGGVA
                     SIFLQFSAGRIVDHFGYLPLLIICGSGYLLAVAVIQLLAPRLEPAEIDGVQPGGSEPI
                     IAH"
     gene            4495..6339
                     /locus_tag="VLI90_16240"
     CDS             4495..6339
                     /locus_tag="VLI90_16240"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HSV15813.1"
                     /translation="MVFVSLCIPNLSMAAVKPFDLTDVRLLDGPSKDKLDANRKYLLS
                     LDADRLLHVFRLNAKLPTTAEPLGGWEKPDSELRGHFVGHYLSGCALMYESTGDEEVK
                     ARAEKIVAALAECQQALGGEYLSAFPESFFDRLEAGKKVWAPYYTIHKIMAGLLDVYQ
                     ATGNQQALDVCTGMGRYFAKRNAKLSDEQMRIVWRNEFGGMMEVAYNLYAITTDEQFK
                     QLAEKFDQNTFFDPLANKTDNLTKLHANTHIPKVIGAARKYELTGEQRFRDIAEFFWD
                     EVALHRAYVTGGTSNHEHWRTPPDHLAGELDFLTQETCCTYNMLKLTRQLMTWDPQTK
                     YADYYERAYINSIWPSMDEPTGMVTYFQSLKPGGYRLFSTPLNSFWCCNGTGVETWSK
                     IADSVYFHDDDSLYVEQFVPTELKWKDRGVTVRQETKFPQAQTTTLHITVEKPQRLTI
                     KLRGPWWAGDRGEVTVGGQPLANVHVVANTYLALDNTWSKGVHHITVQLPMRLHLEPM
                     PDDPTLAAVMYGPVVLAGKLGTENLKRDDLFKPDQTKYTQGAIDVPPLLADGENLGDS
                     IKPIDGHPLEFTATTANGPVTLTPIYAVGDERYTVYWRLQQSTTRPVN"
BASE COUNT         1203 a         1995 c         2029 g         1158 t
ORIGIN      
        1 cgccatcagc gataggttgt ccaggctgta gcccagcgcc tgcatcgcaa cgaaggtcaa
       61 cagcagcgac agcggcaggg ccacggcggg gatcagcgtg tcggtggccc ggccgaggaa
      121 ggcgaagatc accagcacga ccagcacgaa cgcgatgaag agcgtttcct gcacgtcgtc
      181 gatgctgtgc acgatcgaca gcgagcggtc gtagatcgtg tggatctgca ccgcgttggg
      241 cagcgtctgc tggatcgttg ggatcagatc ttcaacggcc ttggaaacgc tgacggcgtt
      301 tgcgccggcc tggcggaaga cggcgatgac gacggtggcg gtcggcacct ggcggtcgcg
      361 cacccagaag cgcaggtcga tgcgctcgtc ctgcaccgaa tcggtcgcgg tggcgacgtc
      421 tttcaaaaag atcggcgcgt tgttgcgaaa gccgatgacc aggttgttgt aggcctcggc
      481 gttttcaagc tggccttgcg gttggagcag gaatgtcttg tgcacgccat cgaactggcc
      541 agcgccttga tagctggtgc cgttctgaat cgccttcgac aggtcttcga tgctcaaccc
      601 gcgcacggcg agggccgtgg gatcggcctt gatgcgcacg gcggagggtg tgccgtacac
      661 ctgcacccgg ctgacgccgg gcacgaggct gatgcgctgc ccggcctgcg tgctggcgta
      721 gtcgtacagc tgaccgcgcg tcacgctgtt gctgaacatc gcgatgtact tgatcggctg
      781 gtcgttcgga ttggtcttgc tcagcgtggg cgggctgggc aggtccggcg gcagttggcc
      841 ggtcgcctgg gtgatggcgg tggacacatc cgtcgcggcg gcgtcgatgc tcttgtccag
      901 cgagaactgc agcgtgaagt tcgtgttgcc ctggccgctg gatgacgtca ccagctccag
      961 cccctggatc tgcatgaact gccgctcgag cggcgtggcg acgttgttcg ccatcgtctc
     1021 cggcgtggcg ccgggatacg cgacgctgac ctggatgacc ggatagtcca cggccggcag
     1081 gtcgttcacg ggcagcgcct tgtaggcgag cacgccgaac aggatggccg agaccgtgag
     1141 cagcacggtc ataacgggtc tacgaataaa cggctcggac aaattcacga ggggccgcct
     1201 tcctgcatag cgacgatttt gtcacgccgg gttcggaggt attccgacga cccggatggt
     1261 tttcgcgcgc cagaaaatcc gggtcgtcgc gcgtacgctt cgaacccggc gtgggggttt
     1321 acgatgcctt tttactattg tggtcctgat cggcgtcggc atgcgcggcg cctggtggtc
     1381 cgcccgcgcc cgtgggtggc gcgatcgtca ccgggccggg gtggtcgggt tgcagcatca
     1441 gctgtcccgt caggacaacg cgttcgccgg cggcgacgcc ttgttcgatc accagcatgt
     1501 ccccctgccg ctggcccggg acgatcgggc ggaactcggc gacgtccgtc gcctggccgt
     1561 ctttttcttc gtgcttgatg acgtaaacgt acggcccctt ctggctgatc tgcgtcgcct
     1621 ggttggggat cagcaccgcg tccttcttca cggtcagcac gagcttcacg tgcacgaact
     1681 ggccgggcca gaagtggcgg tcctgatttg ggatcagcgc ccgcagcttg acggtgccgg
     1741 tgccatcctg cacggcgttg tcgaggaaga cgagctggcc ttcgcgcggt tcgaagtgct
     1801 gcgctgcttg cgcggccacc ggttgagtcg tcgcctgggg catcgcttcc gccaccgccg
     1861 gctgtgtcgt cgcggcctgc gtggtgggcg gtgagccggc gacattcatc ggcgagggtt
     1921 gatcctgcgg cagatccgcc agcaccttca acgtgccctc ggccatgtgc cggcggacga
     1981 gtgtgaggtc gttttcggtg atcgtgaagt cggcgtagat cgggtcgatc ttctggatcg
     2041 tcagcatgtt cgtgccgccg ttgggaccgg acgaccacac gacgttgccg gggtcgatca
     2101 accgatgtcc ggcccggccg tcgatcggcg aatggatttg gcaatagtcg aggttcaact
     2161 tcgcagtctg cacggcggct tcgcccgcct tcaccttggc ctcggcgacc gccagcgcgt
     2221 ttttcttctg gtcgtactcc tgttgcgaga cggcgctcgt gccttgcaga tgttcgacgc
     2281 gggcaaaatc ctgctgcgcg aattcgcgcg cggcctggtt ttccagcagc gtcgcctgcg
     2341 cttgcgccag cccggcttcg aacggccgct tgtcgatctc gaacaggatg cagcccttgt
     2401 tcacatcagc gccgtcttcg aagtgccggg ccatcaacat cccggtcacc tgcggctgga
     2461 tggtcaccgc ttcggtggcg accgcccgac cgactggttc atctaagtag accggcacgt
     2521 ccttcgcctc ggcaaccgcg acggtcacca tcggcggggg catcgggcca gacatggcgt
     2581 tggcgtcgcc tcttcggcag ccgctgaccg ccgcgaggag tgccaaagca gatagtccag
     2641 taattgacgg tcgaatgaat gacgatcgat gcataagaat tcccgtggac gtacttactt
     2701 tacgggttga cgaaccttgg agcctcccgt ggaaggcatc ttacgcgcgc aatgccgagg
     2761 tcggcgaggg gttcgccaaa gattttgtga tcgcgagcct ctcaacacca agtagtcgat
     2821 tttggagcga cgttttcgac gtcgcggccg agaaacacct gtccccgacg cgctcgaccg
     2881 cgacacgact gtcttaacta tcgcatttat ggagacttaa tgtgcaatga tcggctcgga
     2941 cccacccggc tggacgccat caatctcggc tggctccagt cgcggcgcta acagttgaat
     3001 gaccgccacg gcgaggaggt aaccggaacc gcagatgatc agcaggggca ggtagccaaa
     3061 atggtcgacg atgcgccccg cggaaaactg caggaagatg ctggcgacac cgccgaacat
     3121 gccgccgatg ccggtgatgg agccaaccac ccggcggggg aacaagtcgc cggtcagcgc
     3181 gaacaggttt gccgagaacc cctggtgagc cgcggcggcc aagccgatca gcagcacggc
     3241 cacccattcg ttattaaatt tcgtcaagac cgtcacaggc agcacgagca gcgcacagat
     3301 cagcagcgcc gtcttgcgcc cggcgttgac gctccacccc gcgcggatca aaccggcgga
     3361 gagataccca ccgccaatgc ttccgaccgt cgcaaagttg tagatgatga ccagcgggat
     3421 gccgatcgtc ttgatgtcca cgccgaaacg cttgttcagg tacggcgcgg cccagaagag
     3481 ccagaaccac cagatcggat cggtcagcgc cttggccaag ctgaacgccc agacctggcg
     3541 gtgcgggagc aacttcgacc acggcaccgg cgtcaccgcc tcggccgcgt cggaattgat
     3601 gtacgccaat tccgatttgc tcaaccgcgg gtgctcggcc ggcactcgat agagcggcaa
     3661 ccagaacgcg acccagatca gtcccgccgc cccggtgatg atgaacgccg cctgccagcc
     3721 ccaggtgatc gtgatccacg gcaccagcac gggcgcaaga atcgccccga tgtttgagcc
     3781 ggagttgaac agcccgatcg caaacgatcg ctctttcttg gggaaccatt ccgccaccgt
     3841 cttgatcgcc gcggggaagt tgccggcttc ggcaacgccc aggacgaccc ggcaggcgcc
     3901 gaacccaaac gccgttcgag cgaaggcgtg cgcgatgctg gcgaaactcc agaaaatcag
     3961 cgcaacggtg tatcccatcc gcgtgccgaa cttgtcgatg aaccaaccgg cggcgagcag
     4021 tcctatcgcg taagccgcgg agaacgcggc gttgatgtcg ccatagttcg tgtcggtcca
     4081 gccgatcttc tgcttcagtg tcggcgcgag aaccgccagc accgagcgat cgacgtagtt
     4141 cacagtcgtg gcgaagaaca gcagggcgca gatgacccag cgatagcggc cgatggtgcc
     4201 ttcctggcca tattccaaag tattggcggt tgcgtcgcta ccggcggcag ttccggccgc
     4261 cgtggtttgt tgttccataa ctcctcatcc gcaggggtca acgagatgac ggcgaccgga
     4321 caccgcgcgg agcgcgggcg ggcgtgtcgc gaatcagcca tgatcgttaa gcaagtggct
     4381 ggcgtcaatt gaagttaccc gccgcgccgg cgcgtgttgt caccttggca cgcgaccgat
     4441 aaactgcccg aacttccaca aggcggaata atgtatcgcc ccctgctcgc agcgatggtc
     4501 ttcgtgtccc tctgcattcc gaacctctcg atggccgcag tcaaaccgtt cgatctaacc
     4561 gacgtccgcc tgctcgacgg gccgtcgaag gacaagcttg atgccaatcg gaaatacctg
     4621 ctcagcctcg acgcagatcg cttgctgcac gtgtttcgcc tgaacgcgaa gttgccgacg
     4681 acggccgagc cgctgggtgg ttgggagaag cccgattccg aattgcgcgg ccacttcgtg
     4741 ggtcactacc tttccggctg cgcgctgatg tacgaatcga ccggcgacga ggaggtcaaa
     4801 gcccgcgccg aaaaaatcgt ggccgcgctg gccgaatgcc agcaggcgtt gggcggcgaa
     4861 tatctttcgg cgttccccga atcgttcttc gatcgcctcg aggccggcaa gaaagtctgg
     4921 gcaccgtact acacgatcca caagatcatg gccggcctgc tcgacgtgta tcaggcgacc
     4981 ggcaaccagc aggcgctgga cgtgtgcacc ggcatgggcc gctacttcgc caagcgcaac
     5041 gcgaagctca gcgacgagca gatgcgcatc gtctggcgaa acgaattcgg cgggatgatg
     5101 gaggtcgcgt acaacctcta cgccatcacc acggacgagc agttcaagca gctggcggaa
     5161 aaattcgacc agaacacgtt cttcgatccg ctggcgaaca agacggacaa cctgaccaaa
     5221 cttcacgcca acacgcacat cccgaaggtg atcggcgccg cgcgaaagta cgagctgacc
     5281 ggcgagcagc gcttccgcga catcgccgaa ttcttctggg acgaagtcgc gctgcaccgc
     5341 gcgtacgtga ccggcggcac gagcaaccac gagcactggc gcacgccgcc cgatcacctg
     5401 gcgggcgaac tcgatttcct cacgcaggaa acctgctgca cgtacaacat gctcaagctc
     5461 acgcggcagc tgatgacgtg ggacccgcag acgaaatatg ccgactatta cgagcgcgcg
     5521 tacatcaact cgatctggcc aagcatggat gagcccaccg gtatggtgac gtatttccag
     5581 tcgctgaaac ccggcggcta tcgtttgttt tccacgccgc tgaacagctt ctggtgctgc
     5641 aacggcaccg gtgtcgagac ctggtcgaag atcgctgaca gcgtctactt tcacgacgac
     5701 gatagcttgt acgtcgagca gttcgttccg acggaactga agtggaagga ccgcggcgtc
     5761 accgtccggc aggaaacgaa attcccccag gcgcagacga caacgctgca catcaccgtc
     5821 gagaaaccgc aacggctgac gatcaagctg cgcggcccct ggtgggccgg cgaccgcggt
     5881 gaagtcaccg tcggcggcca accgctggcg aacgtccacg tcgtcgccaa cacctatctg
     5941 gcgctggaca acacgtggag caaaggcgtc catcacatta cggtccagct gccaatgcgc
     6001 ctgcacctgg agccgatgcc ggatgacccg acgctggctg cggtgatgta tggccccgtc
     6061 gttctcgccg gtaaattggg aacggaaaat ctcaagcgcg acgatttgtt caaacccgat
     6121 cagaccaagt acacgcaggg cgcgatcgac gtcccgccgc tgctggccga tggtgaaaat
     6181 cttggcgact ccatcaagcc gatcgacggc cacccgctgg agttcacggc gacgacggcg
     6241 aacggcccgg tgacgctcac gccgatctac gcggtggggg atgagcgata cacggtgtat
     6301 tggcgtctgc aacaatcgac gacgcgcccg gtgaattagg gcgcgcgctt gtcatcctcc
     6361 agcgcaggcg tactcgccgg agtcg
//
LOCUS       DATIMH010000935         3743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3398085, whole genome shotgun sequence.
ACCESSION   DATIMH010000935 DATIMH010000000
VERSION     DATIMH010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3398085"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VLI90_16245"
     CDS             complement(<1..1120)
                     /locus_tag="VLI90_16245"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSV15814.1"
                     /translation="MAKQDRSALRAGLFIVITLMLIAIVIVSIKGFRTVFTPVAEHKV
                     HFTLADDIGGLRVGDDVRLGGFKVGVIHSIDVVGIAEGEKPGILITFSVPKKYPLYDN
                     ARVMVQGTLTGSSWLNIEKIGDGIPLAEDTELTGYPSVTTEMLAKLRDVAPEVTGLVK
                     EVHAVTLPKVHANLDHAVTTIDSIHAFADRAAEMAIQVRDLFGDTNPDFRATVKHLNS
                     VTASADEKLPGILDHLDGVMVKVGTTIDNAKSALEDIKATAVNLKDLSGNARGVIVNN
                     RGKLDGIIASLKTTGDNLKGASAEVRRSPWRLLYKPAPDELENLTLYDAARQFSDGAN
                     DVNDAAMALRDATHNPQVDEAQLKKLVENLNATFANFHE"
     gene            complement(1225..2025)
                     /locus_tag="VLI90_16250"
     CDS             complement(1225..2025)
                     /locus_tag="VLI90_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV15815.1"
                     /translation="MESAIQVLGVTKKFDTRTVLDAIRLDVYRGETLCILGGSGSGKS
                     TLLRLMIGNLPPDGGDIVGLGKSLSKMTDGEMTEYRKSIGVLFQSGALYNSMTVGENV
                     ALPLREHTDLPDETIEIMVKIKLELVGLRRHADKMPAELSGGMKKRAGLARAIALDPK
                     ILFYDEPSAGLDPVTSHEIDILISDLNRKLGVTSVVVTHEMDSAFRIADRMVLLDRGK
                     FIVSGTPDEMKDSTDPLVRQFVHGLIDGPLTERRLADGYEDDLLEGSV"
     gene            complement(2101..2907)
                     /locus_tag="VLI90_16255"
     CDS             complement(2101..2907)
                     /locus_tag="VLI90_16255"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV15816.1"
                     /translation="MLLRPITAIGAFVLGVLEFLGGLAYLIRDTFVASRQGLFSRRAW
                     RFAWKHLWFQADRVGVKSIPIVSLVMMCIGAILALQMAPILRDFGQVQLVANIISIAV
                     FRELGPLVSAVVLTGFAGASIAAEIGSMVVSEEIEALEAEAIDPIRFLVVPRVFATTI
                     MMVCIAVVGDLMGIIGGLLTSHFFLGLNYHQYLSRTFEQVKIKDFHTGLWKAAVFGVL
                     ISSFACFLGLRVTGGATGVGVATTRTVVLTIVALIFVDLMFTAAFYYLGL"
     gene            complement(3001..3333)
                     /locus_tag="VLI90_16260"
     CDS             complement(3001..3333)
                     /locus_tag="VLI90_16260"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSV15817.1"
                     /translation="MIPGSRREGDAAIIAVRGEIDLHNSPELRQEVLALLTDGSIKKL
                     VFNLAEVPYMDSSAIAVMVEALQKLRKIGGKICLTNLQPRVKGLLEIARLDAIFILAK
                     DEADALAR"
     gene            complement(3452..>3743)
                     /locus_tag="VLI90_16265"
     CDS             complement(3452..>3743)
                     /locus_tag="VLI90_16265"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV15818.1"
                     /translation="DVTVERRAGGVTIRIRDWGNGEDPSKLPPREQDPLSPGGIGLIC
                     MKRLMDDVAYVPQADGMLLEMSRTTHGSKAAASDGECDDGCASGATSDRDET"
BASE COUNT          674 a         1208 c         1129 g          732 t
ORIGIN      
        1 cttcgtggaa gttcgcgaac gttgcgttta gattctcgac cagcttcttc agctgcgcct
       61 cgtccacctg tggattatgc gtcgcgtcgc gcagcgccat cgcggcgtcg ttcacgtcgt
      121 tggcgccgtc ggaaaactgg cgggcggcat cgtacagcgt caggttctcc agttcgtccg
      181 gcgcgggctt gtacagcagg cgccacggcg atcggcgcac ttcggcgctg gcgcccttga
      241 ggttgtcgcc ggttgtcttc agcgatgcga tgatgccatc cagcttgccg cggttattca
      301 cgatcacccc gcgggcgttg ccgctcaggt ccttcagatt cacagcggtc gccttgatgt
      361 cctcgagcgc ggacttggcg ttgtcgatcg tcgtgccgac cttcaccatc acgccgtcca
      421 gatgatcgag gatgccgggc aacttctcgt cggcgctggc ggtcacgctg ttcaggtgct
      481 tcaccgtcgc gcggaagtcg gggttggtgt cgccgaacag gtcgcgcacc tggatcgcca
      541 tctcggcagc gcgatcggcg aaggcgtgga tcgaatcgat cgtcgtcacc gcgtgatcca
      601 ggttggcgtg caccttcggc agcgtgaccg cgtgcacttc cttcaccagc ccggtcactt
      661 ccggcgccac gtcgcgcagc ttggccagca tctccgtcgt gaccgacgga tatcccgtca
      721 attccgtgtc ctccgccagc gggatgccgt cgccgatctt ctcaatgttc aaccagctcg
      781 atccggtcag cgtcccctgc accatcacgc gggcgttatc atacagcgga tatttcttcg
      841 gcaccgagaa cgtgatgagg atgccgggct tctcgccttc ggcgatgccg acgacgtcga
      901 tcgagtggat gacgccaacc ttgaacccgc cgaggcgcac gtcgtcaccg acgcgcaggc
      961 cgccgatgtc atcggccaac gtgaagtgca ccttgtgttc cgcgaccggc gtaaacacgg
     1021 tgcggaagcc cttgatgctg acgatgacga tcgcgatcag catcagcgtg atcacgatga
     1081 acagcccggc tctcagggcg ctgcggtctt gtttggccat ggtatttgtc cgttgtcagt
     1141 cgtccgttgt cagtggttgt ggcgcatcac aactgaggtt gtggcgcatc acaactgact
     1201 acggacaact gacaactgac tccttcaaac gcttccttcc aacagatcat cttcataccc
     1261 gtccgccagt cggcgttcgg tcagagggcc atcgatcagc ccgtgcacga actggcgaac
     1321 cagcggatcg gtcgaatctt tcatctcgtc cggcgtgccg ctgacgatga attttccgcg
     1381 atccagcagc accatgcgat cggcgatgcg aaacgccgaa tccatctcgt gcgtgacgac
     1441 gacgctggtc acgccgagtt tgcgattcaa atcgctgatc aggatgtcga tttcatggct
     1501 ggtgaccgga tcgagaccgg cgctgggctc gtcgtagaac aggatcttcg gatcgagcgc
     1561 gatggcgcgg gccaggccgg cgcgcttctt catcccgccg gaaagctcgg ctggcatctt
     1621 gtcggcatgg cggcgcaggc cgaccagctc cagcttgatc ttcaccatga tctcgatcgt
     1681 ctcatccggc agatccgtat gctcgcgcag cggcagggcg acgttctcgc cgacggtcat
     1741 cgagttgtag agcgcgccgc tctggaagag cacgccgatc gacttgcggt attcggtcat
     1801 ctccccgtcg gtcattttgc tgaggctctt gccgaggccg acgatgtccc cgccgtcggg
     1861 tggcaggttg ccgatcatca ggcgaagcaa cgtggacttg cccgacccgg acccgccgag
     1921 gatgcacagc gtttcgccgc ggtagacgtc gagacggatg gcgtccagga ccgtgcgggt
     1981 gtcgaacttc ttggtgacac cgagaacttg gatggcggat tccatggagt tttgcggtcg
     2041 ccgcttgcgg cttcgcagac ccggcggtgt ggtttgcgaa gccgcaggcg gcgggctcaa
     2101 ttacaatccg agataataaa acgccgcggt gaacatcagg tctacaaaga tcagcgcgac
     2161 gatcgtcagc accaccgtgc gcgttgttgc gacacccacg ccggtcgccc cgccggtgac
     2221 gcgcaggccc agaaaacaag cgaaggagct gatcagcacg ccgaacaccg cggccttcca
     2281 caggccggtg tgaaagtcct tgattttcac ctgctcgaac gtgcgcgaca ggtactggtg
     2341 atagttcagg cccaggaaaa agtggctcgt gagcaacccg ccgatgatgc ccatcagatc
     2401 gccgacgacg gcgatgcaga ccatcatgat agtcgtcgcg aacacgcgcg gcaccaccag
     2461 gaagcggatc ggatcgatcg cctcggcctc cagcgcctcg atctcttcgg agacgaccat
     2521 cgatccgatt tcagcggcga tcgacgcgcc ggcaaaaccc gtgagcacca ccgcgcttac
     2581 cagtgggcca agctcgcgga agacagcgat cgaaatgatg ttcgccacca gctgcacctg
     2641 cccgaagtca cgcaggatcg gggccatctg cagcgcgagg atcgcgccga tgcacatcat
     2701 caccaagctg acgattggga tgcttttgac gccgacgcgg tcggcttgga accacaggtg
     2761 cttccacgcg aatcgccagg cgcgtcttga gaacagcccc tgccggctgg cgacgaaggt
     2821 gtcgcggatc aggtacgcga gcccgccgag gaactcgagc acgccaagca cgaacgcgcc
     2881 gatggcggtg atgggacgga gcagcacggt catatgggtt ctaaaaaaca acatcggccc
     2941 gaccggctta agcgggtcgg cctgatttga ttgatcgggc tcgcctgttc ccgcgcgcga
     3001 tcatcgcgcc agcgcatcgg cttcatcctt ggcaaggatg aagatcgcat ccagccgcgc
     3061 gatttccagc aagcccttca cgcgcggttg aagattcgtc aggcagatct tcccgccgat
     3121 cttccgcagc ttctgcagcg cctcgaccat caccgcgatc gcgctgctgt ccatgtacgg
     3181 cacctcggcc aggttgaaca ccagcttctt gatgctgccg tcggtcagca gcgccagcac
     3241 ttcctgccgc agttcggggc tgttgtgcag gtcgatctcg ccgcgcaccg cgatgatggc
     3301 cgcgtcgcct tcgcggcggg atccgggaat catcgggcta atgggtttac tttcgctcat
     3361 cgaatctcac gttcccgact tggcctgcag gcgcccgcac gcgacttccc cggcaatatc
     3421 atacgcagcc cgcgcgatcg tgcaaaacga atcatgtctc atctcgatcg ctcgtcgcac
     3481 cactcgcaca gccgtcatcg cactcgccat cgctggcggc ggccttgctg ccgtgggtcg
     3541 tgcgcgacat ttccaaaagc atgccatccg cctgcggaac gtaggcgacg tcgtccatca
     3601 accgcttcat gcagatcaaa ccaatgccgc cgggcgacag cggatcctgt tcgcgcggcg
     3661 gaagcttgga cggatcttca ccgttgcccc aatcgcggat gcgaatggtg accccgccgg
     3721 cgcggcgttc gaccgtcacg tcc
//
LOCUS       DATIMH010000936         5365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1090426, whole genome shotgun sequence.
ACCESSION   DATIMH010000936 DATIMH010000000
VERSION     DATIMH010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090426"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="VLI90_16270"
     CDS             complement(<1..1857)
                     /locus_tag="VLI90_16270"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSV15819.1"
                     /translation="MPKNLIESRRDCATCHLEWSTEFEQPQAPLLIDKPAKPQAAQED
                     TCLGCHDGSVGDSRKIVWREHSHKVGMKPPAEMKIPPGLPLEDGKITCRTCHTAHAVP
                     GTEDDLGGTFFLRVDNSQSQLCQLCHGDKSGGPQHGSHPVGPMTVGLPDKLKNAGAKS
                     GPKGQELICQSCHGAHGSKADKLLVMSTSESQLCVSCHEKIKPGMWREGAPHDHPQNP
                     PLETDAQRQAIKDMGTHIGTGDRLVCLSCHKLHESPSPRKLLADTLKDSKLCLRCHPD
                     RADMVASQHDLRKTAPAEKNRLGQTPDDSGPCGACHSFHQYARDRAPAKGDPTGVCTT
                     CHDKGHVAEKKGETFAFDHPSAVPKDKLPRDLKLAVMTDELDPAKVDVACLTCHDPHK
                     AKTTKFLRNKPDDLCGTCHTEAKTLAGKHDFTDRPELKNAQGKTAAETGKCGFCHSVH
                     KATSNLVMWNGTKDSPQRPEQLCVQCHKTGGMAKDHPAPPFSHPMGPQPNATTRPAMA
                     ALPLFDEAGHRSATGNVACASCHNPHSDSTKSALLLRVTGSTSALCVQCHSEKKTLAG
                     STHDASGNPKAWPAAAQSVNHDMCMTCHRAHSNDQQRKLWAMTPAPAAAPADG"
     gene            complement(2019..2915)
                     /locus_tag="VLI90_16275"
     CDS             complement(2019..2915)
                     /locus_tag="VLI90_16275"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HSV15820.1"
                     /translation="MPPIPIEQRIVATITKTFAAPDANAMHMPTDLAIDSHGNIIVAD
                     GANDRLAQFKSEGAFVGYLTNIGEDYFARPVGVTFDAKDNLWVADTGNHRVLIISAIG
                     QVTERIALPKGDEGKLAGPTSVAVARDGSRAYIIDNANHRVLIRDNRTGAITAMGKSG
                     RALGQFEFPFMTCIGLQNYVFITEAVGGRVQQVSPDDRWAGTIGHVGVTLGDLYRPKG
                     LTTDARGQLYVGDSSLNVIQAFSPRGVVIGVLTDGDGQPLQFQHPMGMRFDKQGNLYV
                     VELRANRVAVVSIQSSPPATKP"
     gene            complement(3058..4170)
                     /locus_tag="VLI90_16280"
     CDS             complement(3058..4170)
                     /locus_tag="VLI90_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15821.1"
                     /translation="MSDRPSKIGALARLPRNLALATWNHRGALKRAARLVFIRAYAAM
                     ILLAITAGGFFAVRYLVRLVFFPPTLPPRFVQWQGNLEVASLRKTDQPGVEMPAMRSP
                     IGHYHGVDRWFQPDNTNGCTLSGCHEPLPHGEKSKVPAFANFHTTFLQCQMCHAPAAN
                     HPASASWVNTSTARTQSTPAILQLLAYLELNADAIQNDPASVSPKIVALLRESINILG
                     GDALLDGLLAQIETSEPGSPVWKHAMIELTAELPLHARGEYRAKLAWSETADGGMAKF
                     AKLTEQSKQYLPLPKDDPQRKELNAAIHGGLDKEAATCLSCHGDKPAMIDFTAVGYTP
                     KRAAFLGQLEVARLMQQIRRGERFFIPNMVGAENAK"
     gene            complement(4163..>5365)
                     /locus_tag="VLI90_16285"
     CDS             complement(4163..>5365)
                     /locus_tag="VLI90_16285"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HSV15822.1"
                     /translation="QCHSNPAIQAKFKLPDVTMSYLQSFHGKATQLGSQETANCIDCH
                     VGQMQNVHVMKAHSDPSSPTSAAHLPDTCRTSQCHRLAGQKISTAAVHLDLSRDRGPE
                     YFIAVGFILLIVLTFGPSLLLTVLKMVQIIVGRKDPQEHHFHKLAHKLMALPEGREKL
                     KRFTVHQRVQHWYLAITFITLVLTGFPMKFADQAWAAWLINVFGGISIARRIHHIAGA
                     MLIVGLFYHAIYVARVVRRQAKSTGQNPLRIFFGLPMMVSPSDMLQMNGLMLYLLGLK
                     STRPSGGRFNPEEKFEYIGVFWGSIVLGTTGVLMWFHAWTTSHLPGRVITIATLFHTM
                     EAFLALLHVGIVHMASVIFAPGVFPVSKAMFNGDTAPEEMAEAHTGMLSDAEELLKES
                     TGQEAVDV"
BASE COUNT          860 a         1615 c         1790 g         1100 t
ORIGIN      
        1 gccgtccgcc ggggcggccg cgggggcggg ggtcattgcc cacagctttc gctgttgatc
       61 gttgctgtgc gcgcgatggc aggtcatgca catgtcatgg ttaaccgact gcgcggccgc
      121 gggccaggcc tttggattgc cgctggcatc gtgcgtgctg ccggcgagcg ttttcttttc
      181 gctatggcac tgaacgcaca aagccgacgt gctgccggtg acgcgcaata gcagcgcgga
      241 cttggtcgag tccgagtgcg ggttgtggca cgaagcgcac gcgacgttgc cggtggccga
      301 gcgatgcccc gcttcgtcga atagcggcaa cgccgccatc gccggccgag tcgtcgcgtt
      361 gggttgtgga cccatcgggt ggctgaaggg cggcgcggga tgatccttcg ccatcccgcc
      421 agtcttatgg cactgcacgc acagctgctc gggacgctgc ggcgagtcct tggtgccgtt
      481 ccacatgacc aggtttgacg tcgccttgtg aacgctgtgg cagaacccgc acttgccggt
      541 ctcggcggcg gtcttgcctt gcgcgttctt cagctccggt cgatcggtga aatcgtgctt
      601 gccggccagc gttttcgctt cggtatggca cgtgccgcac aggtcatctg gcttattgcg
      661 aaggaacttc gtcgtcttcg ccttgtgcgg gtcgtggcac gtcaggcagg cgacatcgac
      721 tttggccgga tcgagttcgt ccgtcatcac cgccagcttc aaatctcgcg gcagcttatc
      781 cttcggcaca gcggatggat gatcgaacgc gaatgtctcg cccttcttct cggcgacatg
      841 gcctttgtcg tggcaggtcg tgcagacgcc ggtgggatct cccttggcgg gcgcgcgatc
      901 gcgcgcgtac tgatggaacg agtggcacgc cccgcacggg ccggaatcat ccggcgtctg
      961 gccgagacgg ttcttctccg ccggcgcggt tttgcgcagg tcatgttgcg acgcgaccat
     1021 gtcggcgcgg tcgggatggc agcgtaaaca gagcttgctg tcctttaatg tatcggccag
     1081 cagcttgcgc ggcgacgggc tttcgtgcag cttgtggcag gacaggcaga ccagccgatc
     1141 gcccgtgccg atgtgcgtgc ccatgtcctt gatggcctgt cgctgcgcat cggtttccag
     1201 cgggggattc tgcgggtgat cgtgcggcgc gccttcacgc cacatgcccg gcttgatctt
     1261 ctcgtggcag ctgacgcata gctggctttc gctggtggac atgaccagca gcttgtcggc
     1321 cttcgacccg tgcgcgccgt ggcaggattg gcagatcagc tcctgacctt ttggtccgga
     1381 tttggcgccg gcgtttttca gtttgtcggg aagcccgacc gtcattggac ctaccgggtg
     1441 tgagccatgc tgcggcccgc cggacttgtc gccgtggcag agttggcaca gctggctttg
     1501 gctgttgtcg acgcgaagga agaacgtgcc acccaaatcg tcttcggtgc ccggcaccgc
     1561 gtgggcggtg tggcaagtgc ggcaggtgat cttgccgtct tccaacggaa gtcccggcgg
     1621 gatcttcatc tcggctggcg gtttcatgcc gaccttgtgg ctgtgttcgc gccagacgat
     1681 cttgcgcgaa tcaccaaccg aaccgtcgtg gcaaccgagg caagtgtcct cctgagccgc
     1741 ctgcggcttg gcgggtttgt cgatcagcag cggcgcctgc ggctgttcga actcggtaga
     1801 ccattcgagg tggcatgtcg cgcaatcgcg gcggctttcg atgaggttct tgggcatttg
     1861 cgcgcgggct gtcgtcggag ccaggagtag cgccaagagc gccaagacgc caagagctaa
     1921 ctcgtagcac gggcgtcccg cccgtgccga ggtgctcaga cacgggcgag acgcccgtgc
     1981 tacctgatga acgaagttga acgtgcacac gttcatggtc agggcttggt tgcgggcggt
     2041 gacgattgaa tgctcaccac cgccacgcgg tttgctctta gttccacgac atataggttg
     2101 ccttgcttgt cgaagcgcat gcccatcggg tgctggaact gcagcggctg gccatccccg
     2161 tcggtcaata cgccgatgac gacgccgcgg gggctgaagg cttggatgac gttgagcgat
     2221 gagtcgccga cgtacagttg cccgcgggcg tcagtggtca ggcctttggg tcgatacagg
     2281 tcgcccagcg tcacgccgac gtgcccgatg gtgccggccc agcggtcgtc ggggctgacc
     2341 tgctgcacgc gcccgccgac ggcttcggtg atgaagacgt agttctgcag gccgatgcag
     2401 gtcatgaagg ggaattcgaa ttgccccagc gctcggccgg acttgcccat cgcggtgatc
     2461 gcgccggtgc ggttgtcgcg aatgagcacg cggtggttgg cgttatcgat gatgtacgcg
     2521 cgcgagccat cgcgcgcgac cgcgacgctg gtgggaccgg ctaacttgcc ttcgtcgccc
     2581 ttgggcagcg cgatgcgctc ggtcacctga ccgatggccg agatgatcag cacgcgatga
     2641 ttgccggtgt cggcgaccca caggttgtcc ttcgcgtcaa acgtcacgcc gaccgggcgg
     2701 gcgaagtagt cttcgccgat gttggtcagg tagccgacga acgcgccttc ggatttgaac
     2761 tgcgccagcc gatcgtttgc accgtcggcg acgatgatgt tgccgtggct gtcgatcgcc
     2821 aggtcggtcg gcatgtgcat cgcgttagca tcgggggcgg cgaaggtttt ggtgatggtg
     2881 gcgacgatgc gttgttcaat cggaattggt ggcaccgctc ttactgtcga tgcgattgac
     2941 agcatcgcgc agagcacgag aagcgcgccg ccaaagccca cggctctgcc gtgggacgta
     3001 tttcgcgccc cgtcccaggg cagagccctg ggccttggtc cagaaaatct gtccgcttta
     3061 tttcgcattc tccgctccca ccatgttggg gatgaagaac cgctcgccac gccggatctg
     3121 ttgcatcaat cgcgcgactt ccaattgccc caggaacgcg gcgcgctttg gcgtgtatcc
     3181 cacggcggtg aagtcaatca tcgcgggctt gtcgccgtgg caactcaggc acgtggcggc
     3241 ttccttgtcc aacccgccgt gaatcgccgc gtttagttcc tttcgctgcg gatcgtcctt
     3301 gggcagcggc agatattgct ttgattgctc ggtcaacttc gcgaacttcg ccatcccgcc
     3361 gtcggcggtt tcgctccatg ccagcttcgc gcgatattcg ccgcgggcgt gcaggggtag
     3421 ctcggccgtc agctcgatca tcgcgtgctt ccacaccggg ctgccgggtt cggacgtttc
     3481 gatctgcgcg agcaaaccgt cgagcagcgc gtcgccgccg aggatgttga ttgactcgcg
     3541 cagcagcgcg acgatcttcg ggctgacgct ggcgggatcg ttctggatcg cgtcggcgtt
     3601 cagctcgagg tacgccagca actgcaggat ggccggcgtc gattgcgttc gcgcggtcga
     3661 cgtgttgacc cagctggcgc tggccgggtg attggcggcg ggcgcgtggc acatctggca
     3721 ttgcaggaac gtcgtgtgga agttggcgaa ggccggcacc tttgacttct cgccgtgcgg
     3781 cagcggttca tggcagccgc tcagcgtgca gccgttggtg ttgtccggtt ggaaccagcg
     3841 gtcgacaccg tgatagtgac cgatcggcga gcgcatcgcc ggcatttcga caccgggttg
     3901 atccgtcttg cgaagcgatg ccacttccaa attgccttgc cactgcacaa accgcggcgg
     3961 cagcgttggc gggaagaaga ccagccgaac cagataccgc acggcgaaga atccaccggc
     4021 ggtgatcgcg agcaggatca tcgcggcgta ggcgcggatg aacaccagcc tcgccgcgcg
     4081 cttcagcgcg ccgcgatggt tccacgtcgc cagcgccagg ttccgcggca atcgcgcaag
     4141 cgcaccgatc ttcgaaggac gatcagacat cgaccgcctc ctgtccggtt gattccttca
     4201 acagctcttc ggcatcgctc aacatgccgg tgtgtgcttc ggccatctct tcgggcgctg
     4261 tgtcgccgtt gaacatcgcc ttggagaccg ggaacacgcc gggcgcgaag atgacgctgg
     4321 ccatgtgcac gatgccgaca tgcagcaggg cgaggaacgc ctccatcgtg tggaacaacg
     4381 ttgcgatggt gatcactcgg ccgggcaggt ggctggtggt ccacgcgtgg aaccacatca
     4441 acacaccggt ggtgccgagc acgatcgatc cccagaagac gccgatgtat tcgaactttt
     4501 cttcgggatt gaaccgccca ccgctggggc gggtggactt caggccgagc aggtaaagca
     4561 tcaggccgtt catctgcagc atgtcagatg ggctgaccat catgggcagg ccgaagaaga
     4621 tgcgcaatgg attctgaccg gtggacttcg cttgccggcg gacgacgcgg gcgacgtaga
     4681 tcgcgtgata gaacaggccg acgatcagca ttgccccggc gatgtggtgg atgcgccgcg
     4741 cgatgctgat cccgccgaag acgttgatga gccatgccgc ccacgcctgg tcggcgaact
     4801 tcatcgggaa gcccgtcagc accagcgtga tgaaggtgat cgcgagatac cagtgctgca
     4861 cgcgctggtg cacagtgaag cgcttaagct tttcgcggcc ctccggcagc gccatcagct
     4921 tgtgggccag cttgtggaag tgatgttcct gcgggtcctt tcgcccgacg atgatctgca
     4981 ccatcttcag caccgtcagc agcagcgacg gcccgaacgt cagcacgatc agcaggatga
     5041 aaccgacggc gatgaaatat tctggcccgc gatcgcgtga caagtccagg tgcactgcgg
     5101 cagtgctgat cttttgcccg gccagtcgat ggcattggct ggtgcggcag gtgtccggca
     5161 aatgcgcagc gctggtcggt gatgacggat ccgaatgcgc cttcatcacg tgaacgttct
     5221 gcatctgccc gacgtggcag tcgatgcagt tcgccgtctc ctggctgccc aattgcgtgg
     5281 ccttgccgtg gaagctctgg agatagctca tcgtgacgtc gggcagcttg aacttcgcct
     5341 ggatggcagg attgctgtgg cactg
//
LOCUS       DATIMH010000937         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3689734, whole genome shotgun sequence.
ACCESSION   DATIMH010000937 DATIMH010000000
VERSION     DATIMH010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3689734"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VLI90_16290"
     CDS             <1..596
                     /locus_tag="VLI90_16290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV15823.1"
                     /translation="MVPNAVDVEQFNAPPRGKQATPTVGVMYSIAEFKGCDVSLKAFE
                     LAKQRISNLKLVTFGNREPFPRLPLPRGAEHTVRPPQDQIKDIYARCDAWLFGSRSEG
                     FGLPLLEAMACRTPVIATPTGAAPELTAPGGGVLVPMEDPAAMAGEIERIVQLPDDQW
                     RVMSDIAYETATKYTWDDATARFEAALGRAMAKAESR"
     gene            complement(898..>2472)
                     /locus_tag="VLI90_16295"
     CDS             complement(898..>2472)
                     /locus_tag="VLI90_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15824.1"
                     /translation="AAHASIGAGRELVASLRCVNCHKSDAAVTGGMPELRQDAPNLSV
                     AAERLRPQWLAYWISNPRSLRPDATMPRVLHDLPAGNAPAVDPRAQDIAAYLTANAPA
                     GGDDAASSDDSVVGRGGHLFTSLGCVACHVAPAVEDSDATLKRVPLKYVKAKYRPAGL
                     VEFLKKPEAHYAWIRMPDFRLADDEATALASFLLSRAKDDALPANTLAAGDAAKGKQL
                     FESVGCMNCHTVEASGSPRPAMTIELSKADWSRGCAADDAASIGKGVDFSFTADQRAA
                     LRAFGPAHLADLSRDPPPEFAARQMSALRCNACHSIDDRDNSWSNLDDEIQKIEQDLP
                     AAEANGEQQVTDQSRPPLTWVGEKLHAGWMGKFIAGQVPYKPRQWIVARMPGFPARAQ
                     GIANGFAMSHGFAPSESAEPPVDAKLADVGRTLTSRTGFGCVACHGVGDQPPLAPFEA
                     PSINFAHVTDRMRHDFFSRWIRHPQYYLPGTKMPQFSDANGRTAYKNVLGGDASQQYE
                     AIWQYLRAGDQITPPQ"
BASE COUNT          440 a          810 c          797 g          425 t
ORIGIN      
        1 cgatggtgcc aaacgcggtg gatgtggagc agttcaacgc cccgccgcgg ggcaagcagg
       61 cgacgccgac ggtcggcgta atgtattcga tcgcggagtt caaaggctgc gacgtgagcc
      121 tcaaggcgtt cgagctggcg aagcagcgaa tttcaaatct gaagctggtg acattcggca
      181 accgcgaacc gttcccgcgg ctgccgctgc cgaggggggc tgagcacacg gtgcgtccgc
      241 cgcaggatca gatcaaggac atctacgcgc gatgcgacgc gtggctcttc ggcagccgca
      301 gcgaaggctt cggtttgccg ctgctggaag cgatggcctg ccgcacgccc gtcattgcga
      361 cgccgaccgg ggctgcgccc gagctgacgg cgccgggcgg cggcgtgctg gtgccgatgg
      421 aagatcccgc ggcgatggcg ggcgagatcg aacgcatcgt gcagttgcca gatgaccagt
      481 ggcgggtgat gagcgacatc gcgtacgaga ctgccacaaa gtacacgtgg gacgatgcga
      541 cggcgcgatt tgaagcggcg ctggggcggg cgatggcgaa ggcagaaagt cgctagacgg
      601 ggcaactgcg gtttcgagta ccacgccggg ttcgacatcc tgagcaagca cgtactcgtg
      661 cgcagtcgaa ggatcttgcc agcagtgagt gaaccttcag tgcaattcca cccgcttgcg
      721 gcttagcatc tttttattca acaacagagg ctaagccaca agcggctgcg acacgacttt
      781 cgagaataag ctgttcactg aattctcgac tgggtccttc gacgtcggcg aatagccggc
      841 gttcaggatg acagcgcgcg cccgccacca tgggatgcaa catgctctgc gtgcccctca
      901 ttgcggcggt gtgatctgat cgcccgcgcg gaggtattgc caaatcgcct cgtactgctg
      961 gctggcatcg ccccccaaga cgttcttgta cgccgtcctg ccgttggcat cgctgaactg
     1021 cggcatcttg gtgccgggga ggtaatactg cgggtggcgg atccagcgcg agaagaaatc
     1081 gtgccgcatg cggtcggtca cgtgtgcgaa attgatcgac ggcgcttcga atggcgccag
     1141 cggcggttga tcgccgacgc cgtggcaggc gacgcagccg aaacccgtgc ggctggtcag
     1201 cgttcgcccg acatctgcca gctttgcatc gaccggcggc tccgcactct cgctcggcgc
     1261 gaaaccatgc gacatcgcaa aaccgttcgc gatgccctgc gcccgcgctg ggaatcccgg
     1321 cattcgcgct acgatccatt gccgcggttt atatggcacc tgtcccgcga tgaatttacc
     1381 catccacccc gcgtgcagct tctcgcccac ccacgtcagc ggcgggcggc tctggtcggt
     1441 gacctgctgc tcgccattcg cctcggcggc cggcaggtcc tgctcgatct tctggatctc
     1501 gtcgtccagg ttcgaccagc tgttgtcgcg atcgtcgatg ctgtgacagg cgttgcaacg
     1561 cagcgcgctc atctgccgag ccgcgaattc cggcggtgga tcacgcgaca ggtccgccag
     1621 gtgcgccgga ccgaacgcgc gtaacgccgc tcgctgatcg gccgtgaacg agaagtcaac
     1681 gcctttgcca atcgacgccg catcatcggc agcgcagccg cgcgaccaat ccgcctttga
     1741 aagctcgatc gtcatcgccg gtcgcggcga accactcgct tcaaccgtgt gacaattcat
     1801 gcatcccacc gactcaaaca actgtttgcc cttggccgca tcacccgcgg ccagcgtgtt
     1861 cgccggcagc gcatcgtcct tcgcgcgcga gagcaggaac gacgccagcg ccgtcgcctc
     1921 gtcgtccgcc agccggaagt ccggcatgcg gatccacgcg tagtgcgcct cgggcttctt
     1981 caggaactcc accaaccccg ccgggcgata cttcgccttc acgtacttca gcggcacgcg
     2041 cttcagcgtc gcatcggaat cttcaacggc aggcgcaacg tgacaggcga cacaaccgag
     2101 gctcgtgaac agatgcccgc cgcggccgac gacggagtcg tcgctgctag cggcatcgtc
     2161 cccacccgcg ggagcgttgg cggtgagata cgcggcgatg tcctgcgccc gcggatcgac
     2221 ggctggcgca ttgccggctg gcaaatcgtg cagcacgcgc ggcatcgtcg cgtcggggcg
     2281 aagcgaccgc gggttgctga tccagtacgc cagccactgc ggccgcaggc gctcggcggc
     2341 gacggacaaa ttcggcgcat cctgccgcag ttccggcatc ccaccggtca ccgcggcatc
     2401 cgacttgtgg cagttcacgc agcgcagtga agcaacgagt tcacgaccgg cgccaatcga
     2461 cgcgtgagcg gc
//
LOCUS       DATIMH010000938         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3690025, whole genome shotgun sequence.
ACCESSION   DATIMH010000938 DATIMH010000000
VERSION     DATIMH010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3690025"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /gene="secY"
                     /locus_tag="VLI90_16300"
     CDS             <1..984
                     /gene="secY"
                     /locus_tag="VLI90_16300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HSV15825.1"
                     /translation="GYMQSSGLVIKSFLRDHPQAFWFTGIICLTAGSIFLMWLGEQID
                     EYGLGNGVSLIILAGIVARMPNAIGLLATQTNFSANANPAKPINMGKILILVALFVFI
                     VMGSILITQAQRRIPVQQAKHTRGRRVYGGGRQFLPLRVNHGGVMPIIFAQSLMLFPG
                     VLFGWLDNSVFAGHSRFVSFLNDEFRRGSYIYILTELVMIYFFAYFWTTVQFQPKEMA
                     NQLRDYGSFIPGLRPGKRTSDYLEKVMMRITYVGGAFLCIIAVIPTAINAWLGIDFRI
                     TAFLGGTGLLIVVSVALDMVQRIEANLLMRNYGGFLSGPGQRGKRIKGRQY"
     gene            1033..1902
                     /gene="map"
                     /locus_tag="VLI90_16305"
     CDS             1033..1902
                     /gene="map"
                     /locus_tag="VLI90_16305"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSV15826.1"
                     /translation="MDGEPSGDFTSNEQLATSNCCPMPIVLKTRREIELMRHAGQVGN
                     EILEKMRQATVVGVTTQELDDLAAAELAKVGGIGLSRNYPTYREGEGFPGHTCISVNE
                     EVVHGIPGRRALQDGDVVTLDLALKVGGYCCDMAITVGVGTIQPKLQKLLDVTKATLD
                     LAITHMKPGKKWTDIARLMQYNVERNGYSVVREFVGHGVGRSMHEDPKVPNFVTQEQL
                     RGDFKLRPGMTLAVEPMVVMGRRDVELLSDGWTVVTEDRMPAAHFEHTVAVTDTGVDV
                     LTDGRAPVPAGAL"
     gene            2075..2188
                     /gene="rpmJ"
                     /locus_tag="VLI90_16310"
     CDS             2075..2188
                     /gene="rpmJ"
                     /locus_tag="VLI90_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HSV15827.1"
                     /translation="MKVRSSVKRICENCKIVRRKGVVRVICTNTKHKQKQG"
BASE COUNT          451 a          618 c          711 g          430 t
ORIGIN      
        1 gggtacatgc aaagcagcgg gttggtcatc aagtccttcc tgcgcgatca cccgcaagcg
       61 ttctggttca ccgggatcat ctgcctgacg gcgggctcga tcttcctcat gtggctcggt
      121 gagcagatcg acgagtacgg cctgggtaac ggcgtcagct tgatcatctt ggccggcatc
      181 gtcgcccgca tgccgaacgc gatcggattg ctggcgacgc agacgaattt cagcgccaac
      241 gccaatccgg ccaagcccat caacatgggc aagatcctca tcctggtcgc gctgttcgtg
      301 ttcatcgtga tgggatcgat cctgatcacg caggcgcagc ggcggattcc ggttcaacag
      361 gccaagcata cccgcggccg gcgcgtgtat ggcgggggac ggcagttcct tccgcttcgc
      421 gtgaatcacg gcggcgtcat gccgatcatc ttcgcgcagt cgctgatgct cttcccgggc
      481 gtgctgtttg gctggctcga taacagcgtc ttcgccgggc acagccggtt tgtctcattc
      541 ttgaatgacg agttccgccg cgggtcgtac atctacatcc tgaccgaact ggtcatgatc
      601 tatttcttcg cgtacttctg gacgaccgtg cagttccaac cgaaggaaat ggccaaccag
      661 ctgcgcgact acggcagctt catccccggt ctgcgcccgg gcaagcgcac cagcgattat
      721 ctcgaaaaag tgatgatgcg catcacgtat gtcggcggcg cattcctttg catcatcgcg
      781 gtcattccga cggcgatcaa cgcgtggctc ggcatcgatt ttcgcatcac cgcgttcctc
      841 ggcggcacgg ggttgctcat cgtcgtcagc gtcgcgctgg acatggtgca gcgcatcgaa
      901 gccaacctgc tcatgcgaaa ctacggtggg tttttgagtg gaccgggaca gcgaggcaag
      961 cgtattaagg gacgacagta ttgagcatcc gttgctggtt gctagttgct cgttgctagt
     1021 agcaagcgaa gaatggacgg ggagccgagc ggtgacttta ccagcaacga gcaactagca
     1081 accagcaact gctgtcccat gccgatcgtt ttgaaaacac gacgcgagat cgagctgatg
     1141 cggcacgccg gtcaggtcgg gaacgagatt ctggaaaaga tgcgccaggc caccgtcgtc
     1201 ggcgtgacga cgcaggaact ggacgacctg gcggcggcgg agttggcgaa ggtcggcggg
     1261 atcgggctgt cgcggaacta tccaacgtat cgcgaaggcg aaggctttcc cggtcacacg
     1321 tgcatcagcg tgaacgaaga agtcgttcac gggatccccg ggcggcgggc gctgcaggat
     1381 ggcgacgtcg tcacgctcga cctggctttg aaggtcggcg gatattgctg cgacatggcg
     1441 atcaccgtcg gtgtcggcac catccagccc aagcttcaga agctgctgga cgtgaccaag
     1501 gcgacgctgg acctggcgat cacgcacatg aagccgggca agaagtggac cgacatcgcc
     1561 cgcttgatgc agtacaacgt cgaacgcaac ggctacagcg ttgttcgaga attcgtgggc
     1621 catggtgtcg gccggagcat gcacgaagac ccgaaggtgc cgaacttcgt cacgcaggaa
     1681 cagctgcggg gcgactttaa gttgaggccc ggcatgacct tggcggttga gccgatggtg
     1741 gtcatgggcc gccgcgacgt ggaattgctg agcgacgggt ggacggtggt gaccgaagac
     1801 cgaatgccgg ccgcccactt cgagcacacg gtggcggtca ccgacacggg cgtggacgtg
     1861 ttgaccgatg gacgcgctcc ggtgccggcg ggagcgctat gatcgtgctg agtgattacg
     1921 tgaatacgtg aatgaggcgg cgggcttcac gtaatcacgt attcactcat cacggcagac
     1981 ttgaggcgga ttatcgtttt tgctataagg accgattcgc cacttcggca gggcctgccg
     2041 gggtggtctt agtcacgaca gagagaagcg catcatgaaa gtgcgaagca gcgtcaagcg
     2101 aatctgtgag aactgcaaga tcgtccgtcg caagggcgtc gtgcgcgtga tctgtacgaa
     2161 cacgaagcac aaacagaagc agggatgatc gctgaagcga aatcagttgc
//
LOCUS       DATIMH010000939         4063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_222408, whole genome shotgun sequence.
ACCESSION   DATIMH010000939 DATIMH010000000
VERSION     DATIMH010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222408"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(588..2033)
                     /locus_tag="VLI90_16315"
     CDS             complement(588..2033)
                     /locus_tag="VLI90_16315"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15828.1"
                     /translation="MSMPALPARSLKVGLFGIGLDTYWPQFPGLRQRLSGYLSRVAEK
                     LQRPGVEVVNLGLIDTPPKAMDAGHRFRQADVDLIFLYVTTYALSSTVLPVVQRAKVP
                     VIILNLQPAAAIDYAAFNRMGDRTRMTGEWLAFCAACPVPEIANVFNRARITFHQITG
                     TIDDDPACWGEVDEWIDVARVAAVMSHNRLGLMGHYYSGMLDIYTDLALQCATFGGHM
                     EILEVDELSALRRDVSAAQIAERVDDFRQSFDVGSDCPQAELERAARTSVALDRLVAQ
                     HDLGSLAYYYMGTGNADNEDMISSIILGTSLLTARGVPVAGEYEVKNAQAMKIMDSFG
                     AGGSFTEYYAIDFADDVVLMGHDGPGHIAIAQGKTKVRPLQVYHGKVGRGLSVEMSVK
                     HGPITLLSVVEPAGGTLKLLVAEGESVPGPILEIGNTNSRYRFAIGARQFVNDWNRHG
                     PAHHCAVGVGHIATKLKKLAALLNIEVAQVC"
     gene            complement(2163..2525)
                     /gene="queD"
                     /locus_tag="VLI90_16320"
     CDS             complement(2163..2525)
                     /gene="queD"
                     /locus_tag="VLI90_16320"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HSV15829.1"
                     /translation="MIVRLSKSFRFEAAHDLPTFPPDHKCRRLHGHSFRFDVVVEGEV
                     DPAKGYLIDYGDIKKVADPLVRRLDHYYLNEIEGLSNPTSEVLAKWLYDHLKPSLPML
                     AEIIVHETCTSMCEYRGR"
     gene            complement(2779..3756)
                     /locus_tag="VLI90_16325"
     CDS             complement(2779..3756)
                     /locus_tag="VLI90_16325"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HSV15830.1"
                     /translation="MTDPLTIWLNAGLPERAMGVLQSNIAPHKLVIATQRNASNLVPG
                     AADPLLEQADVAYGQPDPKQVVDCPRLKWIHLTTAGYTRYDSAPFREAMRARNTIVTN
                     SSAVYDAPCAEHILAMMLSLARQLPQALDEQRNTRAWSSKPFRARSRLLDGQTAIIYG
                     FGSIARRLVELLAPLRLNMVSVRRTVRGDETVRMVTATEADALLPQADHVIDILPAAE
                     HTDGFFSAERLARIKRGAIFYNIGRGATVDQNALQSALQNGNLAAAYLDVTNPEPLPP
                     DHPLWSLPNCFITPHTAGGSEDEFDRGVRHFLDNLHRFTAGHPLINRVI"
     gene            3879..>4063
                     /locus_tag="VLI90_16330"
     CDS             3879..>4063
                     /locus_tag="VLI90_16330"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HSV15831.1"
                     /translation="MSAMPVMIVVLCAMAIAYRYYSAFLAARVAALDDARATPAELYD
                     DGQNYHPTNKWVLFGHH"
BASE COUNT          740 a         1194 c         1335 g          794 t
ORIGIN      
        1 ccgcagaagc gattcgttgg cctcctggaa atgcgccccg cgcgacacgc cgggatagga
       61 agacgggttc agcctcgtct caaccgcaac gctgtagaag tcgccatccg gcaccgccac
      121 tgcggttcgc ggaacggaca cttctacggc gtgcgcgagt gcgaattcca ggagcgagag
      181 gtgccgcctt atcttgccac gccgggtgca gagatactcc gacgatccgg attctgcggc
      241 gggaaggaag caagtcatcc gggtcgccgg agtacctccg aacccggcgt ggattggttt
      301 ctgtccgctc ctgcaaaagt gtcgggtcgt gagcggcact ccacgcgagg gcagggtcag
      361 atgtcttggt tgccgcagaa tccgggtcct cgcgagcgcg ctccaagtcc ggagaggggt
      421 gttaagccgg gcgggctagg acagatgcga agaagagcgc aacttgcgca acctcaaatc
      481 gctatgttgt atcggacaca gcaatatatc ctctgctgca ccagtcgctt aatgctcaaa
      541 cagcgcgcgc aactttggaa aaatttttct tgcgcggtcc acctgcgcta gcacacctgc
      601 gcgacctcga tgttcagcag cgccgccagc ttcttcagct tcgtggcaat atggcccacg
      661 ccgacggcgc agtgatgtgc cgggccgtgg cggttccagt cgttgacgaa ctgcctcgcg
      721 ccgatcgcga agcgatagcg gctgttggtg ttgccaattt ccaggatcgg gcccggcacg
      781 gattcgcctt cggctaccaa cagcttcaaa gttcccccgg cgggttccac caccgacagc
      841 agcgtgattg gcccgtgctt caccgacatc tccaccgata gcccacggcc gactttgccg
      901 tggtagacct gcagcggccg aaccttcgtc ttgccctgcg cgatcgcgat gtgcccgggc
      961 ccgtcgtggc ccatcagcac gacgtcgtcg gcgaaatcga tcgcgtaata ttccgtgaag
     1021 ctcccaccgg cgccgaagct gtccatgatc ttcatggcct gcgcgttctt cacttcatac
     1081 tcgcccgcca ccggcacacc gcgggcggtc agcaggctgg tgccgaggat gatggaactg
     1141 atcatgtctt cgttgtcggc gttgccggtg cccatgtagt agtaggcgag cgagccgaga
     1201 tcatgctgcg cgacgagccg atcgagcgcg accgacgttc gcgctgcgcg ctccaactcc
     1261 gcctgtgggc aatccgagcc gacatcaaat gactggcgga aatcatccac acgctcagcg
     1321 atctgtgccg ccgagacatc gcgccgcaac gcggaaagct cgtccacctc gaggatttcc
     1381 atatgcccgc cgaatgtcgc gcactgcagc gcgaggtcag tgtagatgtc gagcatgccg
     1441 ctgtaatagt ggcccatcaa cccgaggcga ttatgcgaca tgacagccgc cacgcgggcg
     1501 acgtcgatcc attcgtccac ctcgccccag cacgcggggt catcgtcgat cgtgccggtg
     1561 atctgatgga atgtgatgcg ggcgcggttg aacacattgg cgatttccgg gaccgggcag
     1621 gcggcgcaga aggcgagcca ttcaccggtc attcgcgtgc ggtcgcccat gcggttgaag
     1681 gcggcgtagt cgatcgccgc cgccggttgc agattcagga tgatgaccgg caccttcgcc
     1741 cgctgcacga ccggcagcac ggtggacgac agcgcgtacg tggtgacgta aaggaagatc
     1801 agatcgacgt ccgcctgccg aaagcggtgc ccggcgtcca tggccttggg cggcgtgtcg
     1861 atcagcccga ggttcaccac ttcgacgccg ggccgctgca gcttctcggc gacgcgcgac
     1921 aggtaacccg acagtcgctg ccgaagcccg gggaactgcg gccaataggt gtccaggccg
     1981 atgccgaaga ggccgacctt cagcgagcgt gcgggcaaag cgggcatgga catgagttac
     2041 ttcaaatgcg gcgggctgta catcttcgtg gacggcgacg ttgaagatct catcttcaca
     2101 tcatgcgcga caaaccgttt agtgacgacg tttacccggc cgctaaacgt gaatacccat
     2161 cgctatcgcc cgcggtattc gcacatcgac gtgcacgtct cgtgcacgat gatctccgcg
     2221 agcatcggta gcgacggctt caagtggtcg tacagccact tcgccagcac ctcgctcgtg
     2281 gggttcgaca agccttcgat ctcgttcagg tagtaatggt ccaaccgccg caccagcggg
     2341 tcggcgactt ttttgatgtc gccgtagtcg atgaggtagc ccttggccgg atcgacttcc
     2401 ccttcgacga cgacgtcgaa gcggaaagaa tgcccgtgca gccggcggca cttgtggtcc
     2461 ggcggaaatg tcggcaggtc gtgggcggcc tcgaagcgga aggatttgga gagacggaca
     2521 atcatagttg tggtgatgat tctagcgcgg ctgcgcggaa acggaagatg catgctcttc
     2581 gcgtagcggt agccccgcag gggcacgtgt ttctttttcc gccaagctca aaatcaaatt
     2641 gaaccacgaa gctcacgaag agcacgaagc agaggaaaat atttgcccgt ttgttcttcg
     2701 tgctcttcgt gagcttggtg gttgaacttt tattccgaaa acatatgtcc attcggggtt
     2761 accgctacgc gggcatcttt agatcacgcg gttgatcagc gggtgccccg cggtgaagcg
     2821 gtggaggttg tcgagaaaat gccgaacccc gcggtcgaat tcgtcttcgc tgccgccggc
     2881 ggtgtgcggg gtgatgaagc agttcggcag cgaccagagc ggatgatccg gcggcagcgg
     2941 ttcggggttc gttacatcca gatacgcggc ggcgaggttg ccgttttgca atgctgactg
     3001 cagtgcgttt tggtccaccg tcgcgccgcg accgatgttg tagaagatcg cgccgcgctt
     3061 gatgcgcgcg aggcgctcgg cgctgaagaa cccatcagtg tgctcggcag cgggaaggat
     3121 gtcaatcacg tggtcggcct gcgggagcag cgcatcggct tcggtcgcgg tgaccatgcg
     3181 aaccgtttcg tcgccgcgga cggttcggcg gacgctcacc atgttcagcc gcagcggggc
     3241 gagcaattcg acgagccggc gggcgatgga accgaagccg taaatgatgg cggtttggcc
     3301 atcaagcaag cgagaacgcg cgcggaacgg tttgctcgac caggcgcgcg tgttgcgctg
     3361 ttcgtccagc gcctgcggca gttgtcgcgc cagtgacagc atcatcgcca ggatgtgctc
     3421 agcacacggg gcgtcgtaga cggccgagct gttggtgacg atcgtgttcc gcgctcgcat
     3481 cgcttcgcga aacggtgcgc tgtcgtagcg cgtgtaaccg gcggtggtga ggtggatcca
     3541 tttcagccgg gggcagtcca cgacctgctt tggatcgggc tgaccgtagg cgacgtcggc
     3601 ttgttccagc agcgggtcgg ccgcgccggg aacgaggttc gacgcgttgc gttgggtggc
     3661 aatgacgagc ttgtgcgggg cgatgttcga ttgcagcacg cccatcgcgc gctcgggcag
     3721 gccggcgttg agccagatcg tcagggggtc ggtcatgcgg aagacagata ctcgttttgg
     3781 tgtatctcgg aaagtatgat gtttgcttcg gcggcgggtc ccagggcttg tcccacggca
     3841 gagcggtggg ctttttgggt gtgttgggag agcgcgtcat gtcagccatg cccgtgatga
     3901 tcgttgtgtt gtgcgcgatg gcgatcgcgt atcggtatta cagcgcgttt ctggcggcgc
     3961 gggtggcggc gctggacgac gcgcgggcga cgccggcgga gttgtacgac gacgggcaaa
     4021 actatcaccc gaccaacaag tgggtgctgt tcggccatca ctt
//
LOCUS       DATIMH010000940         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3973150, whole genome shotgun sequence.
ACCESSION   DATIMH010000940 DATIMH010000000
VERSION     DATIMH010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3973150"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VLI90_16335"
     CDS             <1..218
                     /locus_tag="VLI90_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15832.1"
                     /translation="GCSIAACPNVGIAIKPCQITLKVNQGYSTFIRVDGQRLCLATVQ
                     GLTDGTPPGVVIYNVRQPGQDFVEGSA"
     gene            220..648
                     /locus_tag="VLI90_16340"
     CDS             220..648
                     /locus_tag="VLI90_16340"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HSV15833.1"
                     /translation="MNNVSRYRAWRFLYPGLDVPDDQAGMRYAANGGIDMVQYNDSIR
                     QAILLLFSTRPGERVMRPDYGCDIHRLVFSPNDDTTAGLAIYYVQQALDRWEPRVVVL
                     QLDAGRDPEEPERLNVTLQYRVRVTRHTELLSFSVNLVEG"
     gene            652..>1991
                     /locus_tag="VLI90_16345"
     CDS             652..>1991
                     /locus_tag="VLI90_16345"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HSV15834.1"
                     /translation="MPLPLPNLDDRDFEQLVQEATQRIRQSNTAWDDLSPSDPGIVLL
                     EVFAYLTETMIYRLNRLPDKVYIAFLRLLGVSLHPPSAASTMLRFSRARGAADADIDI
                     PRGTRVTLERSGGSTTPPIFMTIQKATLAKGADSVDVPALQAEQIEGEEHNVATGLPG
                     LVIKVLRPPIIYGTGDPLDLIVGVEAAPAELDQRVPAIQFADKTYRIWREVENFANPS
                     EDGFVYLVDRMTGTVSFAPALYEPDDKGIMPDQPAVLGEVPPAGRSIRIWYRRGGGTD
                     GNVAANTLTALKDPIRGLQVTNPAPATGGRAEETLDNALLRGPKELHSLQRAVTADDF
                     ELLALKTQEVARAKAVTRAAMWSYGQRGTVEVILVPDVPDSAWQGDWLPATTLIQYAT
                     DQSRTQVQAALDERRPLGTSCVVSWAHYKSVRVTAKIVVRREDDPEAVKSRVLKR"
BASE COUNT          378 a          621 c          621 g          371 t
ORIGIN      
        1 aaggctgctc gattgcggcg tgtcccaatg ttggcatcgc tatcaagccg tgccagatta
       61 ccctgaaagt taaccagggg tattcgacct ttattcgcgt cgatgggcag cgcctgtgcc
      121 tcgctactgt ccagggattg accgatggca cgccgccggg tgtagtgatc tacaatgtcc
      181 gccagccggg gcaagatttt gtcgagggat cggcctgata tgaataacgt ctcgcgttat
      241 cgcgcctggc gcttcctcta tcccggcctc gatgtgccgg atgatcaggc cgggatgcgc
      301 tatgctgcca acggcggcat cgatatggtg cagtacaacg attcgatccg ccaggcgatt
      361 ctgctgctgt tttcgacgcg ccccggcgag cgggtcatgc gcccggacta cggctgcgat
      421 attcatcggc tggtcttttc gcctaacgac gatacgacgg ctggcttagc gatctattat
      481 gtccagcagg cgctcgaccg ctgggagccg cgcgttgtgg tgcttcaact tgacgccggg
      541 cgcgatccag aggagccaga gcgcctcaac gtgacgctgc aatatcgcgt gcgtgtcacg
      601 cgccacaccg agttactctc tttttcagta aatctcgtgg agggctaatc gatgccgctg
      661 ccactgccta atttagatga ccgcgatttt gaacagttgg ttcaggaagc gactcagcgc
      721 atccgccaat cgaacacggc ctgggacgat ctctcgccga gcgatcccgg catcgtgctg
      781 ctggaagttt tcgcctacct gaccgaaacg atgatttatc gcctcaaccg cctgcccgac
      841 aaggtctata tcgcctttct gcggctgctg ggcgtctcac tgcatccgcc gtcggcggca
      901 agcacgatgc tgcgcttcag ccgggcgcgc ggggcagccg acgccgacat cgacatcccg
      961 cgcggcacgc gcgtgacgct cgaacggagc ggcggcagca ccacgccgcc gatcttcatg
     1021 acgatccaga aagccacact ggcaaaggga gctgactcgg tcgatgttcc ggcgctgcaa
     1081 gcggagcaga tcgaaggcga ggaacacaac gttgcgaccg gattgcccgg tttggtgatc
     1141 aaggtgctgc gcccgccgat catttacggg acgggcgacc cgctcgacct gattgtcggc
     1201 gtcgaagccg cacccgccga actggatcag cgcgttccgg cgatccagtt cgccgacaaa
     1261 acctatcgca tctggcgcga agtcgagaat ttcgccaacc ccagcgaaga tggcttcgtc
     1321 tatctggtgg atcgcatgac gggaacagtc agctttgctc cagccctcta cgagccggat
     1381 gacaagggga ttatgcctga ccagccggct gtgttgggcg aagtcccgcc cgccggacgc
     1441 tcgatccgca tctggtatcg tcgcggtggt ggcacggacg gaaacgtcgc cgccaacacg
     1501 ctgacagcgc tcaaagaccc gattcggggc ttgcaggtga ctaatcccgc gcccgcgaca
     1561 ggagggcgcg ccgaggaaac gctcgacaac gcactcctgc gcgggccgaa ggaactgcac
     1621 tcgcttcagc gcgctgtcac cgccgacgat ttcgaactgc tggcgctcaa gacgcaggaa
     1681 gtcgcacggg cgaaagcggt cacgcgcgcg gcgatgtgga gctacggaca gcgcggcacg
     1741 gtggaggtga tccttgtgcc ggatgtgccg gacagcgcct ggcagggcga ttggctgccc
     1801 gcgacgacgc tgatacagta cgccaccgac cagtcgcgca cgcaggtgca ggccgcgctg
     1861 gacgaacgcc gtccgcttgg cacgtcctgc gtagtatcgt gggcgcatta caaatccgtc
     1921 cgcgtgactg ccaagatcgt cgtgcggcgc gaagatgacc ccgaagcggt caagagccgt
     1981 gtcctcaaac g
//
LOCUS       DATIMH010000941         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3112576, whole genome shotgun sequence.
ACCESSION   DATIMH010000941 DATIMH010000000
VERSION     DATIMH010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3112576"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..>3047
                     /locus_tag="VLI90_16350"
     CDS             383..>3047
                     /locus_tag="VLI90_16350"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HSV15835.1"
                     /translation="MVSPSSAAAHVQQRRWAEHLEPRVMLTGVTSSVAAGTLSITVDD
                     ATGVAITASAGQVKINGSDPGGGASAASGITHITVTASGAFDNAIDFSQVVPGTFSSL
                     TQVTVDADGGTGDALTGPNVAKTWNVTGSGAGTITYAGGPSFSFSNVEQLAGSASADT
                     LSYAGISSGVTVNLKTGAATDFSVGVTGMDNLTGGSGDDNLTGNDNNNVIRGLGGNDT
                     LTPLGGRDSLYGGDGTDQFNGGPSISSTFGDLLFAKLAQIRRLIEGDINNASFTGYVY
                     NKEQVPFISFGQGGNRLQDVEKADVIATFYNVLHTALNGLTTTSDIQQVLYTSLGNSG
                     SGLNILADANANGVSDVDDVTMIVDNTAAGNAKIEFQTPLHKSALNGSGNPNFDTDTQ
                     LDLDTHLPGLPLAGGATTHGGVIVAAGYDYNLAFGVSQSSGVFIDESQPDELHIDLRA
                     ALTVIDGDSTHAIYHDPDGSLGVLYASIEDQPLNHSSMQATLTVNFVDETTAPTVKIN
                     GEADYHLRMATRSTEDGLDTINPIYNANMDATWTFGTAGSPQLLDGTSLLGTKPTITF
                     SSVTMDANSFFQNFVKPIVDDHIRSIFSSIDGLVEVMTYKLPVLGVSMAEVFDNPVLS
                     TALVGNFAAFFNALEVYKDMTFPSPGGTVDLGSFTIQDDARAKNHGPNNDDFVDIADA
                     NITPPAVDPITQLRNKSSTVNSWFDNLDLTFQYGFGGTPGIAPFANATYHLLQFPMFT
                     GHAINSFHMLLGHHADLLKWDVPPLSVFYTFVVKQQLFPPGPEEVDAGDVKAGKTAGL
                     VAFFVLRPSFQAHWAGGYDTHGFEIETPSEGYYLNGTDQSQVHPPSVHTIADDFTQGQ
                     LTTSFMRLNIDVSALVGASLPLGPLNIDIGVG"
BASE COUNT          594 a          980 c          948 g          525 t
ORIGIN      
        1 agcatccggg tcgtcgcgag tacgctccga acccggcgtg gggaagtgcc gcgcgtccct
       61 cgtcacgtcg ccacgctcgc accacgacca tctcatcgtc cgctaatgtg gccgcgcgtt
      121 gagcgggctt tcgtccagat atgtcaatct cagttattcc ttgactcgcg cgcccgttga
      181 gatagattcg ggggacgcgg caatatcttt gcatgttgtc ggacctcccg gccgtttttt
      241 agcgggcgct ggggtgcaca cctcacattt gggctagatc gatttcagga gaggaaaact
      301 catgggcttg tccaccgctc gtcggacgcg ctgcgcttct cgttcccgtt tgggtcatgc
      361 ggttgccagg gcgattgggc gaatggtgtc gccttcgagc gcggcggcgc acgtgcagca
      421 gcggcggtgg gcggagcact tggagccgcg cgtgatgctg accggcgtca cctcgagcgt
      481 tgcggcgggc acgttgagca tcaccgttga tgacgccacg ggcgtggcga tcaccgccag
      541 cgcggggcag gtgaagatca atggctctga ccccggcggc ggcgcgagcg cggccagcgg
      601 catcacgcac atcaccgtca cggccagcgg cgcgtttgat aacgcgatcg acttttcgca
      661 ggtcgtgccc ggcacgttca gcagcctgac gcaggtgacg gtggacgccg atggcgggac
      721 gggcgatgcg ctgacgggac cgaacgtcgc caagacttgg aacgtgaccg gcagcggcgc
      781 ggggacgatc acctacgcgg gtgggccgag cttctcgttc agcaacgtcg agcagttggc
      841 gggcagcgcg tcagccgaca cgctgagcta cgccggcatc tccagcggcg tcaccgtcaa
      901 cctcaagacc ggcgcggcca ccgatttctc ggtcggtgtg accggaatgg acaacctcac
      961 cggtggctcg ggcgacgaca acctgaccgg caacgataac aacaacgtca tccgcggcct
     1021 cggtggtaac gacaccctca cgcccctggg cggccgcgac agcctgtacg gcggcgatgg
     1081 caccgatcag ttcaacggcg ggccgagcat ttcgagcacc tttggcgatc tgctgttcgc
     1141 caagctcgcg cagattcgcc ggctcatcga aggcgacatc aacaacgcga gcttcaccgg
     1201 ctacgtttac aacaaggagc aagtcccgtt catctcattc ggtcagggcg gcaaccgcct
     1261 gcaggatgtg gagaaggcgg atgtcatcgc gacattctac aacgtgctgc acacggcgct
     1321 gaacgggcta acgaccactt ccgacattca gcaggtgctg tacacgtcgc tcggcaatag
     1381 cggcagcggg ttgaacatcc tggccgatgc caacgcgaat ggcgtgtcgg atgtggatga
     1441 cgtgaccatg atcgtggaca acaccgccgc cggcaacgcg aagatcgaat ttcaaacgcc
     1501 gctgcacaag agcgcgctga acggcagcgg caatcccaac ttcgacaccg acacgcagct
     1561 ggacttggac acgcacctgc ccggcctgcc gctggccggt ggcgccacca cgcacggcgg
     1621 cgtgatcgtc gcggcgggtt acgactacaa cctcgccttc ggcgtcagcc aatcgagcgg
     1681 cgtcttcatt gacgaatcgc agccggatga actgcacatc gacctgcgcg ccgccctcac
     1741 cgtcatcgac ggcgacagca cccacgcgat ctatcacgac cccgacggtt cgctcggcgt
     1801 cctgtacgcc agcatcgaag atcagccgct gaaccacagc agcatgcagg cgacgctgac
     1861 ggtgaacttc gtcgacgaga caaccgcgcc aacggtcaag atcaacggcg aagcggacta
     1921 tcacctgcgc atggcgacgc gatcgaccga ggacgggctt gacacgatca acccgatcta
     1981 taacgcgaac atggacgcga cgtggacgtt cggcacggcc ggctcaccgc agctgctgga
     2041 cggcaccagc ctgctgggca cgaagccgac catcacgttc agcagtgtga cgatggacgc
     2101 gaattcgttc ttccagaact tcgtcaagcc gatcgtggac gatcacatcc gctcgatctt
     2161 cagctcgatc gacgggctgg tcgaagtgat gacttacaag ctgccggtcc tgggcgtgtc
     2221 gatggccgaa gtgttcgaca acccggtgct gtccaccgcc ctggtgggca acttcgccgc
     2281 gttcttcaac gcgctggaag tgtacaagga catgacattc cccagccccg gcgggaccgt
     2341 cgacctgggc agcttcacga ttcaagacga tgcgcgcgcg aagaaccacg ggccaaataa
     2401 cgacgacttc gtcgatatcg ccgacgcgaa cattaccccg ccggcggtcg atccgatcac
     2461 gcaattgcgg aacaagagct caaccgtcaa cagctggttc gacaacctgg acctgacgtt
     2521 ccagtacggt tttggcggca cgcccggcat cgcgccgttc gccaacgcga cgtaccacct
     2581 gctgcagttc ccgatgttca cggggcacgc gatcaactcg ttccacatgc tgctgggcca
     2641 tcacgcggac ctgctgaagt gggacgtccc gccgctgagc gtgttttaca cattcgtggt
     2701 gaagcagcag ctgtttccac cgggaccgga agaagttgac gccggcgacg tgaaggcagg
     2761 caagaccgcc gggctggtcg cgttcttcgt gctgcgtccg agcttccagg cccactgggc
     2821 cggtggatat gacacgcacg ggttcgagat cgaaacgccc agcgaagggt attacctcaa
     2881 cggcaccgat cagtcgcagg tgcatccgcc aagcgttcac acgattgccg acgacttcac
     2941 tcaggggcag ctgacgacat cgttcatgcg attgaacatc gacgttagcg cgctggtcgg
     3001 cgcgtcgctg ccgcttgggc cgctcaatat cgacatcggc gtcggcg
//
LOCUS       DATIMH010000942         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1962576, whole genome shotgun sequence.
ACCESSION   DATIMH010000942 DATIMH010000000
VERSION     DATIMH010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962576"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1554
                     /locus_tag="VLI90_16355"
     CDS             100..1554
                     /locus_tag="VLI90_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSV15836.1"
                     /translation="MGSSSRFRFARRRRIIAIILLGVLAYLLSATRPGLVATARAETT
                     AVAADRDDLAQLQDRFQSIAAKVSPAVVAISASVTADASPAAMRSEELNGEKLQAFLA
                     KSTRMVGTGFLITADGYILTNDHVIDDAEQLWITTDDKRVFPAIVVGSDPRADLAVLK
                     IPARDLAAVHLGDGAHVKRGQWSIAIGNPFGLSADGQMCISVGVVSAVNRSLPKLSDK
                     ENRLYQNLIQTTAQINPGNSGGPLFDLNGDVIGVSTAVIMPQKNANGIGFAMPIDDHL
                     LALVQDLKQGKEIVYGYLGLIVTTPTERDRHGAGIADSIGARVDSVQSDSPAAGGQVK
                     ENDILTAIDGKPIADSDAFVQMIGCAPVTRPVQIALVRDGKPMQIAMTLRRRQMPVAA
                     VTHDRQRLRWGGMILGVRGTGGDGLMVFGIDSNSPFAKLGVHEGTIITTVAGKTVSGV
                     IDLQSIINDTPIEQCDLGIAPNANLATAVVPVEH"
     gene            complement(1656..2057)
                     /locus_tag="VLI90_16360"
     CDS             complement(1656..2057)
                     /locus_tag="VLI90_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15837.1"
                     /translation="MEGRSNLFSLFMFAVTLPVTAVVLIAVAPKLAASDDGRLILVLL
                     VAPLSLALVITLIKIVATPAVRYVRLAWLKRHQPDAEYGVTPPRCKGCGYDLRASPDQ
                     CPECGTPVDGMDGTIIRYLIKLRRLDELNRK"
     gene            2200..2547
                     /locus_tag="VLI90_16365"
     CDS             2200..2547
                     /locus_tag="VLI90_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15838.1"
                     /translation="MRFSRTVASLAMALAVVSCKSANNHADKEAEEGNEVKMKLDQVP
                     PAVRDSLTREAGGAQVGDVDKESKDGRTVYETDAMINGTNYEIKVDEAGKLISKKIDN
                     EADEKKGEKEEGK"
BASE COUNT          510 a          865 c          778 g          415 t
ORIGIN      
        1 agagccgggg cgtgtcgccc cggaaccgga ccggcagggc ccggctctgt tgcggctctt
       61 cctttgctaa agcagacgcg ggactatcgg ggcaaccgaa tgggcagcag cagcagattt
      121 cgatttgcgc gccgcaggcg catcatcgcc atcatcctcc tgggcgtgct ggcttacctg
      181 ctcagcgcga cccgccccgg cctcgtcgcc accgctcgcg ccgaaaccac cgccgtcgcc
      241 gcggatcgcg acgatcttgc ccagctccag gaccgcttcc aatccatcgc cgccaaagtc
      301 tccccggccg tcgtcgccat ctccgccagc gtcaccgccg acgcctcgcc ggccgccatg
      361 cgcagcgagg aacttaacgg cgaaaaactc caggcgttcc tcgccaaatc tacccgcatg
      421 gtcggcacgg gttttctaat caccgccgat ggatacatcc tgaccaacga tcacgtaatc
      481 gacgatgccg agcagctgtg gatcaccacc gatgacaagc gcgtcttccc cgcgatcgtg
      541 gtcggctccg acccgcgcgc cgacttggct gtcctgaaaa ttcccgcgcg agatctcgcc
      601 gccgtccacc ttggcgacgg cgcccacgtg aagcgcggcc aatggtcgat cgccatcggc
      661 aaccccttcg gcctcagcgc cgacggccag atgtgcatca gcgtcggcgt cgtcagcgcc
      721 gtcaaccgct cgctgcctaa actgtcggac aaggaaaacc gcctctatca aaacctgatc
      781 cagacaaccg cccagatcaa ccccggcaac tccggcggtc cgctgttcga cctgaacggc
      841 gacgtgatcg gcgttagcac cgcggtgatc atgccgcaga agaacgccaa cggcatcggc
      901 ttcgccatgc cgatcgacga tcacctgctc gcgctggtgc aagacctgaa gcagggcaag
      961 gagatcgtct acggctacct cggcctgatc gtcaccacac cgaccgagcg cgatcgtcac
     1021 ggcgcgggga tcgcggattc aatcggcgcc cgcgttgatt cggttcaatc cgattcaccc
     1081 gccgccggcg ggcaggtgaa ggagaacgac atcctcaccg cgatcgacgg caaaccgatc
     1141 gccgacagcg acgcattcgt gcagatgatc ggctgcgcgc cggtcacccg cccggttcag
     1201 attgcgctgg tgcgcgatgg caagccgatg cagatcgcga tgaccctgcg ccgccggcag
     1261 atgccggtcg ccgccgtcac acatgaccgc cagcgcctgc gctggggcgg catgattctg
     1321 ggcgtccgcg gcaccggcgg tgatgggctg atggtcttcg gcatcgattc caacagcccc
     1381 ttcgccaagc tcggcgttca cgaaggcacg atcatcacca ccgtcgccgg caaaaccgtc
     1441 tcgggcgtga tcgatctgca gagcatcatc aacgacacgc cgatcgagca gtgtgacctg
     1501 ggcatcgcgc ccaatgccaa cctcgccaca gccgtggtgc cggtcgagca ctgattcctt
     1561 ggttagaaaa gcccacggct atcccgtggg acgggatccg agatgcgcgt cccacggcat
     1621 agccgtgggc tttgttgcca gagatcgcgc gtgcttcact tccgattcag ctcatccagc
     1681 cgccgcagtt tgatcaggta tcggatgatc gtcccatcca tcccatccac cggcgtgccg
     1741 cactccggac attgatccgg gctcgcccgc aggtcgtatc cacacccctt gcaccgcggc
     1801 ggggtcacgc cgtattccgc atccggttga tgacgcttga gccacgcgag gcgcacgtaa
     1861 cgcaccgccg gggtcgcgac gatcttgatg agcgtgatga ccagcgccag cgacagcggc
     1921 gcgacgagca gcacgaggat caggcgaccg tcgtcgctgg ccgccagctt cggcgccacc
     1981 gcgatcagca ccaccgcggt gaccggcagg gtgacggcga acatgaacag cgaaaagagg
     2041 ttgctgcggc cttccatgac tgctccatta ttcgcgcgtg tgtgccgtgt tggcaattga
     2101 tcatcccgcc gtcatcttgc gccgttatga tgtcgcaatc gtgagccatc cgccgatgcg
     2161 gatggcgctg cagatcggaa ctggaaagga gccaactcga tgagattctc acgcactgtt
     2221 gccagcttgg ccatggcgct ggccgtcgtt tcgtgcaagt ccgcgaacaa ccacgccgac
     2281 aaggaggccg aagagggcaa cgaagtgaag atgaagctgg atcaagtccc gcccgcggtg
     2341 cgcgactcac tgacgcgcga agccggtggc gcgcaggtcg gcgacgttga taaggaatcc
     2401 aaggacggcc ggaccgtcta cgagaccgac gcgatgatca acggcacgaa ctacgagatc
     2461 aaagtggacg aagccggaaa gttgatcagc aagaagatcg acaatgaggc ggacgagaag
     2521 aagggggaga aggaagaagg gaagtagctc atagcagggg cgtctcgc
//
LOCUS       DATIMH010000943         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5713303, whole genome shotgun sequence.
ACCESSION   DATIMH010000943 DATIMH010000000
VERSION     DATIMH010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5713303"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1208)
                     /locus_tag="VLI90_16370"
     CDS             complement(246..1208)
                     /locus_tag="VLI90_16370"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSV15839.1"
                     /translation="MISAARPPDHGAAILVGTIGGILVAASIAGAILHFTARSDQARR
                     TIANINARIRAWWVMAAVFAGAIAMGPGGACVLFALVSFFALREMLTLAPTRRADHHT
                     MFWAFFVIIPLQYLIIYRTAPLYDLFEIFIPVYCFLFLAIRGTLVGDYKNYLERTAKI
                     QWGVMICVYCVSFAPALLMVDVPGYRQDWRLLVFLVLIVQMSDVLQYCWGKSLGRRKI
                     APTISRNKTWEGFVGGVLSATALGAALYRITPFTPGQAAGISLTIAILGFFGGIVMSA
                     IKRDAGIKDYGHLIAGHGGMMDRIDSLTFAAPVFYRIVRHWFSA"
     gene            complement(1205..1828)
                     /locus_tag="VLI90_16375"
     CDS             complement(1205..1828)
                     /locus_tag="VLI90_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014720582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSV15840.1"
                     /translation="MIPALIRLITGAQARWVGVDPVDPAGAIPQRIYFANHGSNLDAP
                     VIWASLPTQLRRKTRPIAARDYWEKTAIHRYVANRVFRAILIERKKVTASNNPLRPME
                     EALDAGDSLIIFPEGTRTMDDEGEMNDFKPGLWHLAKRHPDVQLVPVYLENLNRILPR
                     GDFLLIPLLAAATFGKPICFDETEAKADFLARAKHAVLELQHNAEAA"
     gene            2211..>2762
                     /locus_tag="VLI90_16380"
     CDS             2211..>2762
                     /locus_tag="VLI90_16380"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HSV15841.1"
                     /translation="MPAPQEHVVRVDLANHAYDITVKPGVLSEIGAKLSKLSPSRRVA
                     LMTDSNVGPRFASSLRASCEAAGIETIAECVIPAGEDHKTFEQITRAYDVMLRAKIER
                     STPILALGGGVIGDMTGFVAATVLRGVPFVQIPTTLLSMVDASVGGKTGVNHAVGKNL
                     IGAFHQPIAVFADPKVLETLPRRE"
BASE COUNT          527 a          827 c          942 g          466 t
ORIGIN      
        1 ccgcaggcgc accaccacac gcggccctgc cagcggaggt acacgatcgc gatgacgaga
       61 agaaggataa cgaaagcgaa cgggcgcgcg ggatgcgccg agcgttcgag aggcagaggc
      121 gcgggatcgg aggagggttt agtcacagcg gtgcgccgaa gaaggcgaag acacgcggta
      181 cgaacgcgag cgtctactgt tcataaccat tactcgcgtg ctcccacgtg tcgatccgca
      241 aatcatcacg cgctgaacca atgccgcacg atgcgataga agaccggcgc ggcgaaggtc
      301 agcgaatcga tgcggtccat catgccgccg tggccggcga tcaggtggcc gtagtctttg
      361 atgccggcgt cgcgcttgat ggcggacatc acgataccgc cgaagaagcc caggatggcg
      421 atcgtcagcg agatgcccgc ggcctggccc ggggtgaacg gcgtgatgcg atacagcgcg
      481 gcccccagcg cggtggcaga taacacgccg ccgacgaagc cttcccaggt tttgttccgg
      541 ctgatggttg gcgcgatctt tcgccggccc aggctcttgc cccagcagta ttgcaggacg
      601 tcgctcatct gcacgatgag cacgaggaag accagcaacc gccagtcctg gcggtagccg
      661 ggcacgtcca ccatcagcag cgccggggcg aagctgacgc agtagacgca gatcatcacg
      721 ccccactgaa tcttggcggt gcgctccagg taatttttgt aatcgccgac cagcgtgcca
      781 cggatcgcga ggaacaggaa gcagtagacc gggatgaaga tttcgaacag gtcgtacagc
      841 ggcgcggtgc ggtagatgat caggtattgc agcgggatga tgacgaagaa ggcccagaac
      901 atcgtgtgat gatccgcccg gcgggtgggg gcgagggtga gcatctcgcg cagcgcgaag
      961 aacgagacca gcgcgaacag cacacacgcc ccgccggggc ccatcgcgat cgccccggcg
     1021 aacacggcgg ccatgaccca ccacgcgcgg atgcgagcgt ttatgttggc aatcgtccgc
     1081 cgcgcttgat cgctgcgcgc tgtgaagtga aggatcgcgc ctgcgatgga ggcggcgacg
     1141 aggatgccgc cgatcgtgcc gacgagaatg gcggcgccat ggtcgggtgg ccgtgcggcg
     1201 gagatcaagc cgcctccgcg ttgtgctgca gttccaacac cgcgtgcttc gcgcgggcga
     1261 ggaaatctgc ttttgcttcg gtttcatcga agcaaatcgg cttgccgaag gtggccgccg
     1321 ccagcagcgg gatcagcagg aaatcgcccc gcggcaggat gcggttcagg ttctccaggt
     1381 agaccggcac cagctgcacg tcggggtggc gtttggccag gtgccacagg cccggcttga
     1441 agtcgttcat ctcgccttcg tcatccatcg tgcgcgtgcc ttcgggaaag atgatcagcg
     1501 aatcgccggc gtcgagcgct tcctccatcg ggcgaagcgg gttgttgctg gcggtgacct
     1561 tcttgcgttc gatgaggatg gcgcggaaga cgcggttcgc gacgtagcgg tggatcgccg
     1621 tcttctccca ataatcgcgc gccgcgatgg gccgggtctt acgccgcaac tgcgtcggga
     1681 gcgacgccca gatgacgggg gcatcgaggt tgctgccgtg attggcgaag tagatgcgct
     1741 gcgggatggc gccggcggga tcaaccgggt caacaccgac ccagcgggct tgtgcgccgg
     1801 tgatgaggcg gatgagggcg gggatcattt tcgggaattc atcctgagcg aaggcggtac
     1861 gccgccggag tcgaaggatc tggccgggaa gtcggtgaac cgtgatccgc gcgcttgcct
     1921 gctcgcgatc ccactaacac tggtctgcga aaccgcaagc gactccctcg cccgactgcg
     1981 agggtaatcg ttcatcgatg tcgcggccag atccttcgac tccggctgag acgtcagggt
     2041 atagcgaacg tcgagaagga aatgactgat tcgtagcacg ggcgtctcgc ccgtgccgaa
     2101 gtgctcaggc acgggcgaga cgcccgtgct acgaaaggca attcgcactt cagccttcct
     2161 cttccttcgt ccaatggagt accacctcca ctgcggctaa actgcacgcc atgcccgcac
     2221 cgcaggagca cgtggttcgc gttgacctgg ccaaccacgc gtacgacatc accgtgaagc
     2281 ccggcgtttt gagcgagatc ggcgccaagt tgtctaagct ctcgccgagc cggcgggttg
     2341 cgctgatgac cgattcgaac gttgggccgc gattcgcgag cagccttcgc gcttcgtgtg
     2401 aggccgcggg gattgagacg atcgctgagt gcgtcatccc cgcgggggaa gatcacaaga
     2461 cgttcgagca gattacgcgg gcgtacgatg tgatgctgcg cgcgaagatc gaacgatcga
     2521 cgccgatcct tgcgctgggt gggggcgtga tcggcgacat gaccggcttc gtcgccgcca
     2581 ccgtattgcg cggcgtgcca ttcgtgcaga tccccaccac cctgctgtcg atggtggacg
     2641 ccagcgtcgg cgggaaaacc ggcgtgaatc acgcggttgg gaagaacctc atcggcgcgt
     2701 ttcatcaacc gatcgcggtc ttcgccgatc cgaaggtgct cgagacgctg ccgcggcgag
     2761 ag
//
LOCUS       DATIMH010000944         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_223359, whole genome shotgun sequence.
ACCESSION   DATIMH010000944 DATIMH010000000
VERSION     DATIMH010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223359"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VLI90_16385"
     CDS             complement(<1..818)
                     /locus_tag="VLI90_16385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV15842.1"
                     /translation="MPAFAVSRCTRTVVLVAANALLATSLLLSGSGCADSLVYAKDAR
                     RDGMALYNEGNYTEAVAAFANATRQDPRDYHSYYYLGASYQAQHQFQQATSAYRSCLD
                     VMPLTLDGKNDTQFRYRTIDALATAMAKSETHGVETVALEQKCAGKASVEEQWMLAKV
                     YRYTGDGDAAIDAYNKAVLIAPTNFTITKEAGLYEQNLGQNDRATFALKKAYAVNPND
                     EQVNDGLRRLGVVLGPSIKDSRDLAQPALPTGPLPEWNPNRSAPGESATIQTPR"
     gene            1140..1961
                     /locus_tag="VLI90_16390"
     CDS             1140..1961
                     /locus_tag="VLI90_16390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV15843.1"
                     /translation="MPLSFVEQVGRGTQDTIAAFGDFCVFCGKAFRWLFTGWFRMKNI
                     RLLLPQLYEVGVKSVPVVGVVGAFIGMVMAVETYAQFKALGQENRLGSVIALSVVKQI
                     GPVMAAVMLAGRVGGALTAELGTMNVTEQLDALRVMGTDPISYLVVPRFLACIFLTPM
                     LTIYSDLLGIVGGWFIAVKTFGIANATFWEYSRRGVEHWQINEGIIKSFFFGGAIGLI
                     ACYKGFTSGAGAAGVGRACTESFVESFIAIIILNFFFAHLAQDWYTTLYGTRSLF"
     gene            2031..>2813
                     /locus_tag="VLI90_16395"
     CDS             2031..>2813
                     /locus_tag="VLI90_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV15844.1"
                     /translation="MSFIELRHVSKRFGRLVVLDNLSLSIEAGRCIVVIGASGTGKSV
                     LLKHIVGLLRPDKGEVWYDGRRIDNLPERALMDVRTDFGFLFQMGALFDSMNVDENVA
                     FPVVQHSNKSADEVLEIARQKLALVGLRETGKKMPAELSGGQKKRVALARAIALDPKV
                     ILYDEPTTGLDPIRSDVINELILKLQRELKVTSIVVTHDMNSALKVGDRIVMLHQGKI
                     VFDGTPDEIKTTEDPVVRRFVQGEASEEELASLRAPSAEQRTT"
BASE COUNT          490 a          850 c          931 g          542 t
ORIGIN      
        1 tcgcgcggcg tctggatcgt cgcgctctcg cccggcgctg agcgattcgg attccactcc
       61 ggcagcgggc cagtcggcag cgccggctgc gccagatcgc gcgaatcctt gatgctgggc
      121 ccgaggacca cccccaggcg gcgcaggcca tcgttcacct gttcatcatt gggattcacc
      181 gcgtacgcct tcttcagggc aaaggtggcc cggtcgttct gcccgaggtt ctgctcgtac
      241 aggccggctt ccttggtgat cgtgaagttg gtcggggcga tcagcaccgc cttgttgtac
      301 gcgtcgatgg cggcgtcgcc gtcgccggtg tagcgataca ccttggccag catccactgc
      361 tcttcgacgc tggccttgcc ggcgcacttt tgttccagcg cgacggtttc gacgccgtgc
      421 gtttcgctct tggccatcgc ggtggccagc gcgtcgatcg tgcgatagcg gaactgggtg
      481 tcgttcttgc cgtccagcgt cagcggcatc acgtccaggc agctgcggta cgcgctggtc
      541 gcctgctgga actggtgctg cgcctgatag ctggcgccga gatagtaata cgagtgatag
      601 tcgcgcgggt cctgccgggt ggcgttggcg aaggcggcga cggcttcggt gtagttgcct
      661 tcgttgtaca aggccatccc gtcgcggcgg gcgtccttgg cgtagacgag gctgtcggcg
      721 caaccgctgc cgctgagcag cagcgacgtc gcgagcagcg cgttggcggc aacaagaaca
      781 acagtgcggg tgcaacggga aacggcaaac gcaggcatgg ctacctccat gtagctgtga
      841 tcgtagaaat ccgcgctgtc cttgaaccga aacgtcgcgc aaacgcgcga atcggtgcga
      901 atttccgggc aattgcgacg gtaccgtacc acggggcgac cgttttgcaa ccacccgagc
      961 gcgaaaattg cgcggtggtt caactaccac gccgggttcg gagggcacgc ccgacgaccc
     1021 ggattctccg gcgcgcaaaa gtatccgggg cgtcgggcgt accctccgaa cccggcgtgg
     1081 ggcgttgatg aaagtcgatc gcgttgccgg ggtcgaactg catccgtatc attcgccgca
     1141 tgcccttgtc gtttgtcgag caggttggtc gcgggacgca ggacaccatc gcggcctttg
     1201 gtgacttctg cgtcttctgc ggcaaagcgt tccgctggct cttcaccggc tggttccgga
     1261 tgaagaacat ccgcctgctg cttccgcaac tgtatgaagt cggcgtgaag tcggtgcccg
     1321 tcgtcggcgt ggtcggcgcg ttcatcggca tggtgatggc cgtcgaaacc tacgcgcagt
     1381 tcaaagcgct gggacaggaa aaccgcctgg gcagcgtgat cgcgctgtcg gtggtcaagc
     1441 agatcggccc ggtcatggcg gcggtgatgc ttgccggccg cgtcggtggc gcgctgacgg
     1501 cggagctggg cacgatgaac gtcaccgagc agctcgatgc gctgcgcgtg atgggcaccg
     1561 atccgatcag ctacctggtc gtgccgcgct ttctggcgtg catcttcctc acgccgatgc
     1621 tgacgatcta cagcgacctg ctcggcatcg tcggcgggtg gttcatcgcg gtcaaaacgt
     1681 tcggcatcgc caacgcgacc ttctgggaat acagccgccg cggcgtcgag cactggcaga
     1741 tcaacgaagg gatcatcaag agtttcttct tcggcggcgc gatcggattg atcgcgtgct
     1801 acaaaggctt caccagcggc gccggcgccg ccggtgtcgg ccgggcatgc acggaaagtt
     1861 tcgtcgaatc tttcattgcc atcatcatcc tgaacttctt cttcgcccac ctggcgcagg
     1921 attggtacac gacgctgtat ggaacccgaa gcctatttta agggttcaga ggtcagggtt
     1981 cagtgaatcg ataaccgtct cttcctgacc cctgacctct gacctctccc atgtccttca
     2041 tcgaactccg ccacgtttcc aagcgattcg gccggctggt cgtgctcgac aacctgtcgc
     2101 tgtcgatcga agccgggcgc tgcatcgtcg tcatcggcgc cagcggcacg ggcaagagcg
     2161 tgctgctgaa gcacatcgtc ggcctgctgc ggccggacaa gggcgaagtc tggtacgacg
     2221 gccggcggat cgacaacctg cccgaacgcg cgctgatgga cgtgcgcacc gacttcggct
     2281 ttctcttcca gatgggcgcg ctgttcgatt cgatgaacgt tgacgaaaat gtcgcgttcc
     2341 cggtggttca gcattcgaac aaaagcgcgg acgaggtgct ggagatcgcc agacagaaac
     2401 tggcgctggt gggcctgcgt gaaaccggca aaaaaatgcc ggccgaacta tccggcgggc
     2461 agaagaagcg cgtcgcgctg gcccgcgcga ttgcgctgga tcccaaagtg atcttgtacg
     2521 atgagcccac gacggggctc gacccgattc gatcggacgt cattaatgag ttgatcctga
     2581 agctgcagcg tgagctgaag gtgacgagca tcgtcgtgac gcacgacatg aacagcgcgc
     2641 tgaaggtggg cgaccggatc gtgatgctgc accagggcaa gatcgtcttc gacggcacgc
     2701 cggacgagat caagacgacc gaagacccgg tggtacggcg gttcgtgcag ggcgaggcga
     2761 gcgaggaaga gctggcgtcg ctgcgggcgc cgagcgcgga acagcgcacg acc
//
LOCUS       DATIMH010000945         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_804976, whole genome shotgun sequence.
ACCESSION   DATIMH010000945 DATIMH010000000
VERSION     DATIMH010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804976"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VLI90_16400"
     CDS             complement(<1..234)
                     /locus_tag="VLI90_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSV15845.1"
                     /translation="MLFDVDSLTYSYQGVAALRELSLEIEEGTRVALLGANGSGKSTL
                     LRLLDGLYFPQSGMVRFRGEDLCEEHFADDTFAF"
     gene            complement(234..1073)
                     /locus_tag="VLI90_16405"
     CDS             complement(234..1073)
                     /locus_tag="VLI90_16405"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HSV15846.1"
                     /translation="MSDLHIHQNPRGNFIEHTIEGLHAALARAMYAENLAGGRGLLQG
                     LDPRVKVAGLLALILATALSLKLWVIAAILLAAVSLVVASALPVKMVSPVWLSVLTFT
                     GVIAFPAIFLTPGATFYKFPNIAWPITVPGITSASFLILRAETSATLAILLVFTTPWT
                     HVLKALRTFRVPIVFVVALGMTFRYILLLLETAQEMFESRKSRTVGKMSRADYRRLAV
                     SSGGVLLGKSFQLSGEVFLAMQARGFRGEVYVLDGFRMASRDWAALALFGALTAACIW
                     AGR"
     gene            complement(1070..>2018)
                     /gene="cbiM"
                     /locus_tag="VLI90_16410"
     CDS             complement(1070..>2018)
                     /gene="cbiM"
                     /locus_tag="VLI90_16410"
                     /inference="COORDINATES: protein motif:HMM:NF008873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HSV15847.1"
                     /translation="RKALHTRLVPLLSLFAAFCFVIMMFNLPLPGGTTGHATGVALAT
                     VVLGPWASMLAVSIALVIQALFFGDGGITAIGANCFNMAIVGSLVAYVVYRAVSGRAP
                     ATSPRRVIAAGLAGYVAINMSALCAAIEFGIQPMLYRDAAGAPLYCPYPLNIAIPAMM
                     IGHLTIAGMAELFVSAGAVAFLQRTDISLLRVPDAASASGGMARTLRPLWATLGVLLV
                     LTPLGILAAGSAWGEWVASDYADPAVRRQIAASSLSAAPPAVAPGGLQRLSTVWTAPF
                     ARYAPPYVRSAGFGYLLSGMFGAGLVVLSGFGIGRVLRR"
BASE COUNT          422 a          703 c          620 g          273 t
ORIGIN      
        1 gaacgcgaac gtgtcgtcgg cgaaatgctc ttcgcacaga tcctcgccgc ggaagcgaac
       61 catgccgctc tgggggaagt acaggccatc cagcaggcga agcagtgtcg atttgcccga
      121 tccgttggcg ccgagcagag ccacccgcgt gccttcctcg atctccaggc tgagttcgcg
      181 taaagcggcc acgccctggt agctgtacgt gaggctgtcg acgtcgaaga gcatcagcgt
      241 cccgcccaga tgcacgccgc tgtgagcgcg ccgaaaagcg ccagcgccgc ccagtcgcgc
      301 gacgccatgc ggaaaccgtc cagcacgtag acctcgccgc gaaagccgcg cgcctgcatc
      361 gccagaaaaa cctcgccgct cagttgaaag ctcttcccca gcaggacgcc gccgctggag
      421 accgccagcc gccggtaatc cgcgcggctc attttgccca cagtgcggct cttgcgcgat
      481 tcgaacatct cctgcgccgt ctccagaagc agcaggatat agcggaacgt catgcccaga
      541 gccaccacga atacgatggg aacgcgaaac gtgcgcaggg ccttcaggac gtgcgtccac
      601 ggcgtggtga acaccagcag aatcgccagg gtggccgatg tctcggcgcg cagaatcagg
      661 aagctcgcgg acgtgatgcc cggaaccgtg atcggccatg cgatgttggg aaacttataa
      721 aatgtcgcgc ccggcgtcag aaagatggcc ggaaacgcga tcacgcccgt aaaggtcagc
      781 acactcaacc agaccggaga gaccatcttg accggcagcg ccgacgcaac caccagcgat
      841 acggccgcca gcaggatcgc cgcgatcacc cacagtttca gcgagagagc cgtcgccaga
      901 atgagcgcca gcagtccggc gaccttcaca cgaggatcga gcccctgcag cagtccgcgc
      961 cctcccgcca ggttttcggc atacatggcc cgcgccaggg cggcgtgcag gccctcgatg
     1021 gtgtgctcga tgaaattgcc gcgcggattc tgatgaatgt gcagatcgct catcggcgga
     1081 gcacccggcc gattccgaac ccgctcagca ccaccagtcc cgccccgaac atacccgaca
     1141 acaggtatcc gaatccggcg ctgcgcacat agggcggagc atagcgggcg aagggagcgg
     1201 tccacaccgt tgacaagcgt tgcagtccgc ccggcgcaac tgcaggcgga gcggccgaca
     1261 gggacgacgc ggcgatctgc ctccgcaccg cgggatcggc atagtccgac gccacccatt
     1321 cgccccacgc cgagcctgcc gccagaattc ccagcggcgt cagaaccagc agcacgccga
     1381 gcgtcgccca caacggacgc agcgtccggg ccattccgcc gctcgcactc gcggcgtccg
     1441 ggacccgcag gagcgatatg tccgtgcgct gcagaaacgc caccgcgccc gccgacacga
     1501 acaactccgc catgcccgcg atcgtcaggt ggccgatcat catggcggga atcgcgatat
     1561 tcagtgggta cgggcagtag agcggcgcgc ccgcggcatc gcgataaagc atcggttgga
     1621 tgccgaactc gatcgccgcg cacagcgccg acatgttgat cgccacatat ccggccaatc
     1681 ccgccgcgat cacgcggcgc ggcgacgtgg cgggcgctcg tcccgaaact gcgcgataaa
     1741 ccacgtacgc caccagcgag cccacgatcg ccatgttgaa gcagttggcg ccgatcgccg
     1801 taatgccgcc gtccccgaag aacagcgcct ggatcaccag cgcgatcgac accgccagca
     1861 tggaggccca gggtcccagg accaccgtgg cgagcgccac gcccgtggca tgtcccgtgg
     1921 tccctcccgg tagcgggaga ttgaacatca tgatcacgaa acagaaggcc gcgaacagcg
     1981 aaagcagtgg caccagtcgc gtatgcagcg ctttgcgg
//
LOCUS       DATIMH010000946         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2245753, whole genome shotgun sequence.
ACCESSION   DATIMH010000946 DATIMH010000000
VERSION     DATIMH010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245753"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="VLI90_16415"
     CDS             <1..1208
                     /locus_tag="VLI90_16415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSV15848.1"
                     /translation="AAELEKVRLSLLNVVKLVPISARHHVTGAADRLLHRLAWTCMWL
                     LIVLVISAGVHFSQYRALIRPLLWLRDDMQRSAANEYKLSVTARGDAEFVAVGALFNG
                     LAKELAELYRSLEAKVIARSRELVRSERLASVGFLAAGVAHEINNPLSVIAGYAELAG
                     KALRRVMMGESANGKGSNALSGAEAEAEAEALSNALQAQTIMHDEAFRCKEITSRLLS
                     LARGGSSDSREELKLDEVARQVAVLVKGLKNYRDRQIIVDLDHSESLEVLANPTEMKQ
                     VLLNLTINALEAVPPLRGEVRITGRRVGQLVELSVQDNGKGMKLETLENVFEPFFTAK
                     RGACEPGTGLGLSITHAIIENHGGRITAESDGPGRGSRFIVHLPAARARVDERALRPR
                     VPAEQAVVA"
     gene            1246..1944
                     /locus_tag="VLI90_16420"
     CDS             1246..1944
                     /locus_tag="VLI90_16420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV15849.1"
                     /translation="MQADTSTTNGKALPTGKEVLVVEDDPRVRNMLASALKEMGFAGT
                     FCASAEAATRAIADGGNFDILILDLNLPGAGGIEFLESVRRQDNDIQTIILTGFGDLE
                     AARKAIHLDVVEFLTKPCTLGSLEVALSRASARRKGQIVDQANQSSDAAIDFGDDPPR
                     APAFARELAATSTIPVAGDESDGSMDEIERKHIFAVLKKNGGNRAATAAELGISVRKL
                     YYRLGEYQRKGLTP"
BASE COUNT          386 a          546 c          736 g          366 t
ORIGIN      
        1 cggctgctga gttggagaag gttcggcttt cacttttaaa tgtcgtcaag ctggttccta
       61 tttccgcgcg gcatcacgtg accggcgcgg ccgatcggct tctccatcgg cttgcgtgga
      121 cctgcatgtg gctgctgatc gtgctggtca tctcggcggg ggtgcacttc tcgcaatatc
      181 gcgcgctgat tcggccgctg ctgtggttgc gcgacgacat gcagcgcagt gccgccaacg
      241 aatacaagct gagcgtgacg gcgcggggcg acgcggagtt cgtggcggtc ggcgcgctgt
      301 tcaacggctt ggcgaaggaa ttggcggagc tgtatcgaag tctcgaggcg aaggtcatcg
      361 cgcgcagccg ggagctggtg cgatcggagc gactggcgag cgtggggttc ctggccgcgg
      421 gcgtggcgca tgagatcaac aacccgctca gcgtgatcgc gggatatgcg gagctggccg
      481 gcaaggcgct tcgccgggtg atgatgggcg agagtgcgaa cggcaaaggg tcaaacgctc
      541 taagcggcgc tgaagctgaa gcggaggccg aagcgttgtc aaacgcactt caggcgcaaa
      601 cgatcatgca cgatgaggcg ttccggtgta aggaaatcac cagccgattg ctgtcgctgg
      661 cgcgtggtgg cagcagcgac agccgggagg aactgaagct ggatgaagtg gcccggcagg
      721 tggcggtgct ggtgaagggg ctgaagaact atcgcgatcg ccagatcatc gtggatctcg
      781 atcacagcga gtcactggag gttctggcga atccgacgga gatgaagcag gtgctgctga
      841 acctgacgat caacgcgttg gaggcggtgc cgccgctgcg gggtgaagtc cgcattactg
      901 gccggcgcgt tggccagttg gttgagctgt cggtgcagga caacggcaaa ggcatgaagc
      961 tggagacgct cgagaacgtg ttcgagccgt tcttcaccgc caagcgcggc gcttgtgaac
     1021 ccggcaccgg tctggggctg tcgatcaccc atgcgatcat cgaaaaccat ggcggtcgca
     1081 ttactgctga aagcgacggc cccggccgcg gcagccggtt tatcgttcac ttgcccgcgg
     1141 cgcgggcgcg ggtggacgag cgggcgctgc ggccgcgcgt gccggccgaa caggcggtgg
     1201 tggcatgacg tagcgttcgt tatagcgatg tgagggcaag tgcagttgca ggctgataca
     1261 agcacaacaa acggcaaggc gcttccaacc ggcaaggaag tgttggtggt ggaagacgat
     1321 ccgcgcgtgc gcaacatgct ggcgagcgcg ctaaaggaaa tgggcttcgc cgggacgttc
     1381 tgcgccagcg ccgaagcggc gacgcgcgcg atcgccgatg gtggcaactt cgacatcctg
     1441 atcctggacc tgaatctgcc cggcgcgggc ggcatcgaat ttctcgagtc ggtccgccgc
     1501 caggacaacg atattcagac gatcatcctc accggcttcg gtgatttgga ggcggcgcgg
     1561 aaggcgattc acctggacgt cgtcgagttc ctgaccaagc cctgcacctt gggcagcctg
     1621 gaagtggcgc tgtcgagggc cagcgcgcgg cgcaaggggc agatcgttga tcaggcgaac
     1681 caatcgagcg atgcggcgat cgatttcggc gatgatccac cgcgtgcgcc ggcattcgct
     1741 cgcgaattgg cggccacgtc aactattccg gtggcgggcg acgagagcga tggttcgatg
     1801 gacgaaatcg aacgcaagca catcttcgcc gtgctgaaga agaacggtgg gaatcgtgcg
     1861 gcgactgctg cggagttggg catcagcgtg cggaagctgt actaccggct tggcgaatat
     1921 cagaggaagg gtttgacgcc gtgagagcaa agcgaacttt agccgacgca aaggcggcgg
     1981 gctttacgct cgtggagctg ctggtggtga tcggcatcat cgcggtgctg atcg
//
LOCUS       DATIMH010000947         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4556320, whole genome shotgun sequence.
ACCESSION   DATIMH010000947 DATIMH010000000
VERSION     DATIMH010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4556320"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VLI90_16425"
     CDS             complement(<1..207)
                     /locus_tag="VLI90_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15850.1"
                     /translation="MRSARLSLVAIFAIVTSLHAQSKTTILTGSAAARRFAYQGQPLH
                     PFCLEFSQERSSRSARSDLVMCSAA"
     gene            570..1343
                     /locus_tag="VLI90_16430"
     CDS             570..1343
                     /locus_tag="VLI90_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15851.1"
                     /translation="MKKALLVTLALLITTRASAQEASGVPLVRERDIVLDVAEQRELR
                     QWTDDVGRYHQWYEQNRNRIAHNVFGFTSDRRELPPVPAWLDGKCDLLGYFDPRPVGE
                     LSAGCDLLSYYRSDFTQDPAAVQAALSQKQNEQDPHASFWKHVHLDAGWTSLDYRMHT
                     YGVVGVHVTLPELAKRVQIFLPPGFLLLAVPDGRGGTTLQPGATIGVSVKMFAFEFPQ
                     GKNGTAYFNLAKAYLISGQSSIGGNAAVDLLGLSFAWGR"
     gene            1557..>2201
                     /locus_tag="VLI90_16435"
     CDS             1557..>2201
                     /locus_tag="VLI90_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15852.1"
                     /translation="MNTLERPRRSRRRRQRRGERRREAAAIVFVTLLALATISLVTWP
                     LWKSPSPSAASGAPSSGAGNIELASVTTIPASTSHPVYKYSVIPGGARNGEELAQAIR
                     HDPVVARHYRSVSPDRVRVERLTQPRRAYVSYRVGDRVYWTNHRVTLPAGELILTDGV
                     TQIRARCGNCISDKAQLPTSGGEPDSAELDTLSPVPLPLEQPLPGTVMAWNSAGA"
BASE COUNT          395 a          730 c          707 g          369 t
ORIGIN      
        1 ggccgcgctg cacatcacaa gatcgctgcg tgccgaacgt gaagaccttt cctgggagaa
       61 ttccaagcag aacgggtgca atggctgtcc ctgatacgcg aagcgtctcg ccgcggccga
      121 accggtgaga atcgtagtct tcgactgcgc atgcagcgac gtcacgatgg cgaagatcgc
      181 gacgagcgag aggcgcgccg agcgcatgtc ctgaattcta gtaccctggc aagtgcgtcc
      241 gctgaattcc agggatcgca cgcaaggccg ccaagctggt ccacgaagat gcagaaccgc
      301 acctcggtac gctcggccgg ccgccggcac cgctggatgc gaggttgatt cgcggatatt
      361 tgacgcgtca aagactagcg ccaatcctga cgccgccggt gcgattgggc gattgacatc
      421 gagcaagggt tcggagacga tcccggccaa actcgcatca gtacgactgc gctcgcgcgc
      481 gaggtggacc ctggatccat cccggcgggc gacgcgtgtc agtgcgcggg tctcggacaa
      541 ttccggggcc gaccgaaccg gggggatcaa tgaagaaggc gttgctcgtc acgctggcgc
      601 tgcttatcac cacgcgcgca tcggcgcagg aggcgagcgg cgtcccgctc gtgcgcgagc
      661 gcgacatcgt cctcgatgtc gccgagcagc gcgagctccg gcagtggacc gacgacgtgg
      721 gccgctacca ccagtggtac gagcagaatc gcaaccgcat cgcccacaac gtcttcggct
      781 tcaccagcga tcgccgcgag ctgccgccgg tgccggcatg gctcgacgga aagtgtgacc
      841 tgctcggata tttcgatcca cgaccggtcg gcgaactgtc cgccggctgc gatctcctct
      901 cgtactatcg cagcgacttc acccaggatc cggcagcagt gcaggcggcg ctgtcgcaga
      961 agcagaacga gcaggatccg cacgcgtcgt tctggaagca cgtgcatctc gacgccggct
     1021 ggacatcgct cgactaccgg atgcacacct acggtgtggt cggcgtacac gtcacgctgc
     1081 cggagctggc gaagcgtgtg cagatctttc tgcccccggg cttcctgctg ctcgccgtgc
     1141 ccgatgggcg cggcggcacc acgctgcagc ccggggccac cattggcgtc agcgtgaaga
     1201 tgttcgcgtt cgagttccct caggggaaga atggaacggc atacttcaac ctcgcgaagg
     1261 cgtatttgat tagcggccaa tcaagcatcg gtggaaatgc ggccgtcgat ctcctgggtt
     1321 tgtcgttcgc ctggggccgc tgaaaggcag gcagcaaggt tccgtgacgc gttatcgccg
     1381 cggcagacga ctatttagcc tggatcctcg gcgtggcgat ggcctcaacg cacgccggcg
     1441 catctcccaa cgaatcctgc ccaccgttgc acagaattgc tgcgcggtct gacatcgaat
     1501 ggcacgtcca acctccttcg gcgcggcata gcccttgcga tatagcgcac tgaatcgtga
     1561 atacactcga acgaccccga cggtcacgcc ggcggcgtca gcgccgtggc gagcggcgcc
     1621 gcgaggcggc agcgattgtc ttcgtcacgc tgctcgccct cgcgacgatt tcgctcgtca
     1681 cctggccgct gtggaaatcg ccgtcgccat cagccgcgag tggggcgccg tcgagcggcg
     1741 ccggcaacat cgagctggcc agcgtcacca cgattcccgc gagcacctcg catcccgtct
     1801 acaagtactc ggtcatcccc ggcggtgcgc gcaacggcga ggagctggcg caggcgatcc
     1861 gtcacgaccc ggtggtcgcc aggcactatc gatccgtatc ccccgaccgc gtccgcgtcg
     1921 aacgtttgac gcagccccgg cgcgcctatg tgtcgtatcg cgtcggcgac cgtgtgtatt
     1981 ggacgaatca ccgggtcacg ctgcccgcag gcgaactgat cctgaccgac ggcgtcacgc
     2041 agattcgcgc gcgctgcggc aactgcattt cggacaaggc gcagctgccg acttcgggcg
     2101 gcgagcctga cagcgccgaa ctcgataccc tgagccccgt tcccctgcca ctggagcagc
     2161 cgctgccagg tacggtgatg gcctggaaca gcgccggtgc g
//
LOCUS       DATIMH010000948         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3692476, whole genome shotgun sequence.
ACCESSION   DATIMH010000948 DATIMH010000000
VERSION     DATIMH010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3692476"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VLI90_16440"
     CDS             <1..197
                     /locus_tag="VLI90_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15853.1"
                     /translation="REEAEKAGRVFVETKATRASKRRDKDEPPKKQQKQGGIKGFLAD
                     LQAKAEEVRREAERRGKDRA"
     gene            309..1622
                     /locus_tag="VLI90_16445"
     CDS             309..1622
                     /locus_tag="VLI90_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSV15854.1"
                     /translation="MLTGDTIAAISSAAGPAARMILRTSGPRAHDIARSIVTGVPSQP
                     SIASRTELTFANLRCPAWLYSFRSPHSYTGEDLVEFHIPGSPALAKMLLDELLARGAR
                     LADPGEFTARAYFSGKIDLAEAEGVAAAIAASNEQELQAARQLLAGELARRLRPAMES
                     IAQTLALIEVGIDFSEEDVTFLATHEIRLRVEEIDADLRRLLEESTRFDQLVHEPRIV
                     FVGRPNAGKSTLINALTGRQRSIVSDIAGTTRDVLSADLDLPSGRVQLLDVAGIDPDE
                     QASHSIDSQMREHALRAIESADRVVLVRDATDASPALELPRTPDLVIRSKSDLAPPQT
                     SVGGISISAISGAGMSNLRDALDRLAFGNESGGAGLALNRRHIASIEQARAALARLAQ
                     SLDGGAAELLALELRESLDALGGVLGSITPDDVLGRIFAGFCIGK"
     gene            1660..2124
                     /locus_tag="VLI90_16450"
     CDS             1660..2124
                     /locus_tag="VLI90_16450"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSV15855.1"
                     /translation="MSDLQTQIVETWQIHNRVNLYLLDAIETDALTLSLKEKGRTIAA
                     LFAHVHNVRLMWLKPAAPSLLDGLDKLDDAATKAQIQIALTNSGDAMAQLLQESASTG
                     KVKSFKRGVVPFLGYLVAHESHHRGQIGWILKHTGHPLDRAIAFGLWDWNAR"
BASE COUNT          430 a          768 c          658 g          340 t
ORIGIN      
        1 aacgcgaaga agccgagaag gccggccggg tgttcgtgga aaccaaggcc acgcgcgcca
       61 gcaaacgccg cgacaaagac gaaccgccca aaaagcagca aaagcagggc gggatcaaag
      121 gtttcctggc cgacctgcaa gccaaagcgg aagaagtcag gcgcgaagcc gaacgccgcg
      181 gcaaagatcg cgcgtgacgc gccgacataa gatcacccca tttagcccgg cgtgaacaca
      241 cgccgggttt gcgcgggact tcagggccga ccgatcgaga aatcggatca cgttcaaacc
      301 agttagagat gctcacaggc gacaccatcg ccgccatcag cagcgccgcc ggccccgcgg
      361 cccgcatgat cctccgcacc agcggcccgc gcgctcacga catcgcccga tccattgtca
      421 ccggcgttcc atcacaaccg tcaatcgcat cgcgcacaga acttaccttc gccaacctgc
      481 gctgcccggc gtggctgtac tcgtttcgct ccccgcacag ttacaccggc gaagacctcg
      541 tcgagttcca catccccggc agcccggcgc tggcgaagat gctgctggac gagttgctgg
      601 cccgcggcgc ccgcctcgcc gacccggggg aattcactgc ccgcgcttat ttctccggca
      661 agatcgacct ggccgaagcc gaaggcgtcg ccgcggcgat cgcagcgagc aacgagcagg
      721 aactgcaagc ggcccgtcaa ctgctcgccg gcgaactggc ccgccgcctc cggcccgcga
      781 tggagtcgat cgcgcagacg ctggcgctga tcgaagtcgg catcgacttt tccgaagagg
      841 acgtcacgtt cctcgccacc cacgaaatcc gcctgcgcgt cgaagagatc gacgcggacc
      901 tgcgtcggct tctggaggaa agcacgcggt ttgaccagct cgtgcacgag ccgcggatcg
      961 tcttcgtcgg ccggcccaac gcgggcaaga gcacgctgat caacgcgctc acgggccggc
     1021 agcgatcaat cgtctccgac atcgccggca cgacccgcga cgtgctgtcg gcggatcttg
     1081 atctaccgag cggccgcgtg cagctgctcg acgtcgcagg gatcgatcca gatgagcaag
     1141 cgtcgcactc catcgattcg cagatgcgcg agcacgcgct gcgcgcgatc gaatcagctg
     1201 atcgtgttgt gctcgtccgc gatgcgaccg atgcgtcgcc ggcgctggag cttcctcgca
     1261 caccagactt ggttatccgc tcgaagagcg atcttgctcc tccgcaaacg tcagtcggcg
     1321 ggatatcgat cagcgcaatc agcggcgcgg gcatgtcgaa cctgcgcgac gcgctcgatc
     1381 gcctcgcctt cggcaacgaa tccggcggcg ctggcctggc gctgaatcgc cggcacatcg
     1441 cgtccatcga acaggcgcgg gccgcgctcg ctcgcctggc gcaatcgctc gatggcggcg
     1501 ccgccgaact gctcgcgctg gagctgcgcg aatccctcga cgccctcggc ggcgtccttg
     1561 gcagcatcac ccctgacgac gtcctcggcc gcatcttcgc cggcttctgc atcggaaaat
     1621 gactcgcggc gcgcgggaac gcgaatatat tgtcccagaa tgtccgacct gcaaacccaa
     1681 atagtcgaga cgtggcagat tcacaatcgc gtcaacctgt atctgctcga tgccatcgaa
     1741 accgatgcgc tgacgttgtc gctgaaagaa aagggccgaa cgatcgccgc gctgttcgcg
     1801 cacgtgcaca acgtccgcct gatgtggctc aagcccgccg cgccgtcgtt gctcgacggt
     1861 ttggacaagc tggacgacgc tgcaaccaaa gcgcagatcc aaatcgcgtt gaccaattca
     1921 ggcgacgcga tggcccagct gctgcaagag tcggcatcca ccggcaaggt caagtccttc
     1981 aagcgcggcg tcgtgccgtt cctcggctac ctggtcgccc acgaatccca ccaccgcggc
     2041 caaatcggct ggatcctcaa gcacaccggc catccgctcg accgcgccat cgcctttggg
     2101 ctctgggact ggaacgcgcg gtgacgtcga aatcagaacc acgccgggtt cggagcgtgc
     2161 tcgcgacgac ccgatatttt ttcccgcgcg ccgaag
//
LOCUS       DATIMH010000949         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3975376, whole genome shotgun sequence.
ACCESSION   DATIMH010000949 DATIMH010000000
VERSION     DATIMH010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3975376"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VLI90_16455"
     CDS             complement(<1..201)
                     /locus_tag="VLI90_16455"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSV15856.1"
                     /translation="MPRELTLRQITVRVRRRGYRTRRITVINTLLDPMAYPAAEIAQL
                     YARRWEVQTCFRHLKQTLDLEHL"
     gene            complement(655..2181)
                     /locus_tag="VLI90_16460"
     CDS             complement(655..2181)
                     /locus_tag="VLI90_16460"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HSV15857.1"
                     /translation="MPDAFIEYQNITKKFAGVTALDAVTFSIARGECHALMGENGAGK
                     STLAKVLAGIHRADEGRVIIDGKEQAFHSPAEAMRAGVGMVHQELAFCPDLTVAENLN
                     MGQYPRRLGMLDRKAMNRRARELLAKIGATSIDVTKCMREMSTAQEQLVQISSAVGTG
                     AKILVFDEPTSSLSEPEAQQLFKLIEQLKSAGVTIIYVSHRMPEVFRLCDRVSVLRDG
                     KYVGTLDRKDATQDRVVQMMIGRSVSDYFPQHLSDKVGETLMSVRNLSSPGKFTDISF
                     EVRKGEIVGFAGLVGAGRSEIAKAIFGLDKDAVGEVRLEGEPLALGSVKRSIGRGIGL
                     VPEDRKRQGLVLMMSCRANASMAVLDRLRRMGILDRKKEREEAGRLFTQMRVKAPSME
                     TPVAALSGGNQQKVAVAKWLIRNCKLLIVDEPTRGVDIGAKAAIHQLLDDLARGGVGI
                     MLISSELPEVLNLSTRILVMRDGKLVGEVPRKGATQEGVLRLMAGVETAPLKTPDAGV
                     "
     gene            complement(2261..>3014)
                     /locus_tag="VLI90_16465"
     CDS             complement(2261..>3014)
                     /locus_tag="VLI90_16465"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSV15858.1"
                     /translation="DAQKQAEAIDALARNGAAGIAVSCTEARTLTPAINKAVQKGVMV
                     MCFDSDAPKSKRFAYYGTDDMSCGQRVMRDLATAMGDKGTIAILAGNQSAPNLQIRVQ
                     GVKDELAKHPNIKLLEAGGGVFYHEETPEKAAEALQTAQNANPSIEGWALIGGWPLFT
                     ANALPWEPGKIKVVSVDALPAQLAYLRDGHVQELLAQDCYGWGHRSVEILLDKIINKK
                     DPPSLRVIDPLTSVTKDNVDEFAKNWEKWLGK"
BASE COUNT          473 a         1027 c          908 g          606 t
ORIGIN      
        1 caggtgctcc aggtccagcg tctgcttgag gtggcgaaag caggtctgga cttcccatcg
       61 ccgcgcgtac agctgcgcga tctccgcggc cggataggcc atcgggtcca gcagcgtgtt
      121 gatgacggta atccggcggg tgcggtaacc gcggcgtcgc acgcgcacgg tgatctgccg
      181 cagcgtcagc tcgcgtggca tgccgatcca ccgccgcagg ctcaaggccg agccccgttc
      241 cttgggccgc tgccagcgca gcagcaggtc ctgctgctcg tgctgccctt gccgctacgt
      301 cgtcgtcggc ggcgcggttg atcgcatcgc aggtgtcgcg cgtgggtcga ggagcagttg
      361 aacagcaaca cctgcatgat ccacaggtgc accgtcgccg ccggtccgag cttgcgctgg
      421 cgccaacgat gaccgcttgc ccgcgcttca cgctcgatgc gatcggccgg cagcagccgg
      481 tccagttccc gtttgacccg cttgacgcat cgactgatac tgctaaccag cctcctgttg
      541 agtgtggctt aaaagcaccc acatcttcgc aggaggccat gcgtcgcgca agcgacccta
      601 acgcgcgtag cggcgcggag ttgtagctaa tctgaatggc attctcggag tacctcagac
      661 accggcgtcg ggggttttca aaggcgctgt ttccaccccc gccatcaggc gcaacacccc
      721 ttcctgcgtc gcgccttttc ttggcacctc accgaccagc ttcccgtcgc gcatcaccag
      781 gatgcgcgtc gagaggttca gcacctccgg caactccgac gagatcagca tgatgccgac
      841 gccgccgcgc gccaggtcgt ccagcaattg gtgaatcgcg gctttggcgc cgatgtccac
      901 gccgcgcgtc ggttcatcga cgatcaacag cttacagttg cggatcagcc acttcgccac
      961 cgcgaccttc tgctggttgc cgccgctgag ggcggcgacc ggcgtttcca tcgacggcgc
     1021 cttcacgcgc atctgcgtga ataatcgccc ggcttcttcg cgttccttct tgcgatcgag
     1081 gatgcccatt cgccgcaggc ggtcgagcac cgccatcgac gcgttcgcgc ggcagctcat
     1141 catcagcacc agcccctgcc gcttgcggtc ttccggcacc agcccgatac cccggccaat
     1201 cgatcgcttc acggaaccca gcgccagcgg ctcgccttcg agcctcacct cgcccaccgc
     1261 gtccttgtcc agtccgaaga tggccttggc gatctcactt cgccccgcgc cgaccaagcc
     1321 ggcgaagccg acgatttcgc ctttgcgcac ctcgaacgag atgtcggtga actttccggg
     1381 cgatgagaga ttccgcacgc tcatcagcgt ctcgccgact ttgtccgaca ggtgctgcgg
     1441 gaagtaatcg ctgaccgatc gcccgatcat catctgcacc acgcggtcct gcgtggcgtc
     1501 tttgcggtca agcgtgccga catacttgcc gtcgcgcagc acgctgacgc ggtcgcacag
     1561 gcggaagacc tcgggcatgc ggtgcgaaac gtaaatgatc gtgacgccgg ccgatttgag
     1621 ctgctcgatc agcttgaaca gctgctgcgc ttcgggctcc gacaacgagc tggtgggttc
     1681 gtcgaagacg aggatcttgg cgccggtccc gacggccgac gagatctgca ccaactgctc
     1741 ctgcgcggtc gacatctcgc gcatgcactt ggtcacgtcg attgacgtcg cgccgatctt
     1801 cgccagcagt tcgcgcgccc ggcggttcat cgccttgcga tccagcatcc ccagccgccg
     1861 cgggtactgg cccatattca gattttccgc gacggtcagg tccgggcaga acgccagctc
     1921 ctggtgcacc atgccaactc cggcccgcat cgcctcggcc ggcgaatgaa acgcctgctc
     1981 cttgccatcg atgatgacgc gcccctcgtc cgcgcgatgg atgccggcga gcactttggc
     2041 cagcgtcgat ttgcccgccc cgttctcgcc catcagtgcg tggcattcgc cgcgcgcaat
     2101 tgagaaggtc accgcgtcca gcgccgtgac gccggcgaat ttcttggtga tgttttggta
     2161 ttcgatgaac gcgtcgggca tatgtgggaa gcgatgaacg atgagggatg aacgatgaag
     2221 gctggttcat ctttcatcgc tcatcgttca tcgctcttcg ttattttccg agccacttct
     2281 cccaattctt cgcgaactcg tccacgttgt ccttcgtcac gctggtcagc gggtcgatca
     2341 cgcgaaggct tggcgggtcc ttcttgttga tgatcttgtc cagcaagatt tcgaccgagc
     2401 gatgacccca gccatagcag tcctgggcca gcagctcctg cacgtggccg tcgcgcaggt
     2461 acgccagctg tgccggcagc gcatcgaccg aaacgacctt gatcttcccc ggttcccacg
     2521 gcagcgcgtt ggcggtaaac agcggccagc cgccgatgag cgcccagcct tcgatgctgg
     2581 ggtttgcatt ctgggcggtc tgcaatgcct cggccgcctt ctcgggcgtc tcctcgtgat
     2641 agaacacacc gccgcccgct tcgagcagct tgatgttcgg atgcttcgcc agttcatcct
     2701 tcacgccctg cacgcggatc tgcaggttcg gggcgctctg attgccggcg aggatcgcga
     2761 tcgtgccctt gtcgcccatg gccgtcgcca agtcgcgcat gacgcgctgg ccgcagctca
     2821 tgtcgtcggt gccgtagtag gcgaagcgtt tgctcttggg cgcgtcggaa tcgaagcaca
     2881 taaccatcac gcccttctgc acggccttgt tgatggccgg ggtcagcgtg cgcgcttcgg
     2941 tgcaggagac ggcgatgccg gcggcgccgt tgcgggcgag cgcgtcgatc gcctccgcct
     3001 gcttttgcgc gtct
//
LOCUS       DATIMH010000950         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_516087, whole genome shotgun sequence.
ACCESSION   DATIMH010000950 DATIMH010000000
VERSION     DATIMH010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516087"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1225
                     /locus_tag="VLI90_16470"
     CDS             38..1225
                     /locus_tag="VLI90_16470"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSV15859.1"
                     /translation="MTDTDERLRRWRLILGGNEADGIKFSLSASDDSMDRALAALYDA
                     EKQGGARGASGKRSGGLGASMPSVARWLGDIRSFFPSSVVRVMQQDAMERLNLRQMLL
                     EPEMLETVEPDVHLVADLLSLSGVMPGKTKQTARIVVRKCVEEIERRLANPLRQAVNG
                     ALNRSARNRRPRHSEIDWNRTIRANLRHYQPEYKTIVPEQRIGFGRKRSSLRDIILCI
                     DQSGSMATSVVYSGIFGAVLASLPAVRTHVVVFDTAVVDLSDKLSDPVEVLFGTQLGG
                     GTDINRALGYCQGLIRQPSDTILILISDLIEGGVRENLLKRAAAIIGSGVHMITLLAL
                     SDDGAPMYDHQNAAALAALGSPAFACTPDMFPDLIGAAIQRIDIATWAAAHDISTAGG
                     ATR"
     gene            complement(1279..>2069)
                     /locus_tag="VLI90_16475"
     CDS             complement(1279..>2069)
                     /locus_tag="VLI90_16475"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HSV15860.1"
                     /translation="ASFMGVYSTLTMRGWNEELCAAVGAQHRLLPEVREGDAIGGRIT
                     HDAARRFGLSAGTPMTVGIVDTSGALLLHGAHVGQLLNVCGSTDVLALCTDRPKPHER
                     LLTRAVGVGRRWVQVGTIGAAGSALAWANRQLFPDLSETDFRKLMRELARKPIKSSVR
                     FDPYLAGDRTSVEQRTGAFTGLTLSSTREQMLGAMIESLAAASAERIPLLAATGTKMR
                     RNVILSGGLQGGLADVLHRDWPGRWTFQQEDEATLRGLAKIQLK"
BASE COUNT          350 a          700 c          682 g          337 t
ORIGIN      
        1 ccactgccgc ggtcttccgg caaaacggag gtgcatgatg accgacactg acgaacgcct
       61 ccgccgctgg cggttgatcc ttggcgggaa tgaagccgac gggattaagt tctcgctgag
      121 cgcgagcgac gactcgatgg accgtgcgct cgcggcgctc tacgacgcgg agaaacaagg
      181 cggggcgcgc ggagcctctg gcaagcgatc gggaggactc ggcgcatcga tgccgtccgt
      241 tgcccgctgg ctgggcgata tccgctcgtt cttcccgtcc tccgtcgtgc gcgtcatgca
      301 gcaggacgcg atggagcggc tgaacctgcg gcagatgctg ctggaaccgg agatgctgga
      361 gacggttgag ccggacgtgc acctggtggc ggacctgctg tcgctctccg gcgtgatgcc
      421 gggcaagacc aagcagacgg cgcggatcgt cgtgcgcaag tgcgtcgagg agatcgaacg
      481 ccggctggcc aacccgttgc ggcaggcggt gaacggtgcg ctgaatcggt cagcgcgcaa
      541 ccgccggccg cggcacagcg agatcgactg gaaccgcacg atccgcgcga acctgcggca
      601 ctatcagccg gagtacaaga cgatcgtgcc ggagcagcgc atcggttttg gccgcaagcg
      661 ctcgtcgctg cgcgacatca tcctttgcat agaccagtcg gggtcgatgg ccaccagcgt
      721 cgtctacagc ggcatcttcg gcgcggtgct ggcatcgctg ccagcggtgc gaactcacgt
      781 cgtcgtcttc gacaccgcgg tcgtcgatct gtctgacaag ctttccgatc cggttgaagt
      841 ccttttcggc acgcaactgg gcggcgggac cgatatcaac cgcgcgctgg gctactgcca
      901 aggcctcatc cgccagccca gcgacacgat cctgatcctg atcagcgacc tgatcgaagg
      961 cggcgtgcgc gagaacctgc tgaagcgggc cgcggcgatc atcggcagcg gcgtgcacat
     1021 gatcacgctg ctggcgctga gcgacgacgg cgcgccgatg tacgaccatc aaaacgcggc
     1081 cgcgctggcg gcgctgggct cgccggcgtt cgcgtgcacg ccggatatgt tcccagattt
     1141 gatcggggcg gcgattcagc ggatcgacat cgccacatgg gcggcagcgc atgacatttc
     1201 gactgcgggc ggcgcgacga ggtagcacgg acgccagagc ctgtgccaag gtgcttcggc
     1261 acgggcgagg cgcccgtgct acttaagttg tatcttcgct aatccccgca gcgtcgcttc
     1321 gtcttcctgc tgaaacgtcc aacgcccagg ccaatcgcgg tgcagcacat ccgctagtcc
     1381 accctgaagt ccgccggaaa ggatgacgtt gcgacgcatt ttggtgccgg tcgccgcgag
     1441 cagggggatg cgctcagcgc tagcggcggc cagcgattcg atcatcgccc cgagcatctg
     1501 ctcgcgcgtg ctcgacagcg tcagaccggt gaacgcgccg gtgcgctgct caacgctcgt
     1561 ccgatcgccc gccaaatagg gatcgaaccg caccgatgat ttgatcggct tccgcgccag
     1621 ctcgcgcatc aactttcgga aatccgtctc ggatagatct ggaaacagct gccgattcgc
     1681 ccacgccagc gccgaccctg cggcgccgat ggtgcccacc tgcacccatc gccggcccac
     1741 gcccacggcc cgcgtcagca gccgctcgtg cggcttgggt cggtccgtgc acagcgccag
     1801 cacatccgtc gaaccacaga cgttcagcag ctggcccacg tgcgcgccgt gcagcagcag
     1861 cgcgccgctg gtgtccacga tgccaacggt catcggcgtg ccggcgctga gcccgaaccg
     1921 gcgcgctgcg tcgtgcgtga tccgcccgcc gatcgcgtcg ccctcgcgca cttccggcag
     1981 cagccggtgc tgcgcgccga cggcggcgca tagctcctcg ttccacccgc gcatcgtcag
     2041 cgtcgaatag acgcccatga acgatgcgt
//
LOCUS       DATIMH010000951         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5141513, whole genome shotgun sequence.
ACCESSION   DATIMH010000951 DATIMH010000000
VERSION     DATIMH010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5141513"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VLI90_16480"
     CDS             complement(<1..900)
                     /locus_tag="VLI90_16480"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HSV15861.1"
                     /translation="MSARPLAGLKVLEFTHTVMGPTAGLLFAELGADVIKVEPAPNGD
                     HTRKLGGFGAGFFAAFNRNKRSLAIDLKKPEGQAAIYRLVEKTDIVLENYGPGTMERL
                     KCGYEHFAPHNPRLIYLALKGYLAGPYEHRPALDEVVQFQTGLAWMTGPPGRPLRAGA
                     SVIDIMGAIFGVVAAQAALRERDVTGKGQRVSSALFESAAFLMSTHMAGMAATGLEAR
                     PMPARRGAWAIYDVFNTAADGQLFIGVTSDQQWTRFVEEFNLQDLAADPRMATNVTRL
                     AERSWLIPALQEILAKYPQSEIES"
     gene            complement(900..1859)
                     /locus_tag="VLI90_16485"
     CDS             complement(900..1859)
                     /locus_tag="VLI90_16485"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HSV15862.1"
                     /translation="MSDLPKHCEIHEEGPREGFQIEPGPISTADKIKLIDALSETGLH
                     HIQVCSFVNPRLVPGWADAEAVAAGFTAKPGVHYTGLYFNGNGLDRALAFKDKLTISG
                     SISLTASAGFTKKNLNRTPEENLAQMKRHAETHLAKGIPVSRVGVMAAFGCNYQGDIS
                     PATVVKTLDDGMKIADETGAKIELFSLADTMGWATPVRIERVIAAVREKWPEMKISLH
                     LHDTRGLAVANAHAGLKMGVTRFDSTVGGLGGCPFAGQPKAAGNICTEELVLLCEEMG
                     IDTGIDLDKLIEVGRMAEEIVGHQLPGELIHAGSLDAFRRMAA"
     gene            complement(1872..>2383)
                     /locus_tag="VLI90_16490"
     CDS             complement(1872..>2383)
                     /locus_tag="VLI90_16490"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSV15863.1"
                     /translation="PSGNTTGHATSQAYVTKEGMLMIGASNLRQQKRLWTVLERPEMI
                     KRTNDERHADHDREAKVLAEIMLTRTAAEWEEWLQERHVPASRVRTMGEALADPQLSS
                     RNILHKHESAPGISGSFSVPVAAFKFAHGGAQVDRPPPMFGQHNDEILTELGYSKADI
                     AGFKSEKVI"
BASE COUNT          386 a          712 c          780 g          505 t
ORIGIN      
        1 ggattcgatt tcggattgag ggtatttggc gaggatttct tgcagagccg gaatgagcca
       61 tgaccgttcc gccagccggg tcacattggt cgccatgcgc gggtcggcgg cgaggtcctg
      121 aaggttgaat tcctccacga agcgggtcca ttgctggtcg ctggtgacgc cgatgaacaa
      181 ctgcccgtct gccgcggtgt tgaagacgtc gtagatggcc catgcgccgc ggcgggccgg
      241 catgggacgt gcttcaagtc cggtcgcggc catgccggcc atgtgcgtgc tcatgaggaa
      301 cgcggcactt tcgaacaaag cgctgctgac gcgttggcct ttgccggtga cgtcgcgttc
      361 gcggagtgct gcctgggcgg ccacgacacc gaaaatcgcg cccatgatgt cgatgacgga
      421 ggcgccggca cgcagtggcc gtccgggcgg gcccgtcatc caggcgaggc cggtttgaaa
      481 ttgcaccact tcgtccaaag cgggacgatg ttcgtaggga ccggcgagat agcccttcaa
      541 cgcgaggtaa atcagccgtg ggttgtgggg tgcgaagtgt tcatagccac acttcagccg
      601 ctccatcgtg ccgggtccgt aattctccag cacgatgtcg gtcttttcga ccaggcggta
      661 gatcgccgcc tggccctcgg gctttttcag atcgatggcc aggctgcgct tgttacggtt
      721 gaaggcggcg aagaacccgg cgccgaagcc gccgagtttg cgcgtatgat cgccattcgg
      781 cgcgggctcg actttgatga cgtccgcgcc aagttcggcg aacagcaatc ccgcggtcgg
      841 ccccatgacg gtgtgcgtga attcaagtac cttcaatccc gccaggggcc gagccgacat
      901 caggcggcca tccgcctgaa agcgtcgagg ctgccggcgt ggatcagttc gccgggcaat
      961 tgatggccga cgatttcctc cgccatccgg ccgacttcga tcagtttgtc gagatcgatg
     1021 ccggtgtcga tgcccatttc ctcgcaaagc agcacgagtt cttcggtgca gatgttcccg
     1081 gcggctttcg gctggccggc gaaggggcag ccgccgagac cgccgaccgt tgagtcgaag
     1141 cgcgtgacgc ccatcttgag gccggcatgc gcgttggcga cggcgaggcc gcgggtgtcg
     1201 tgcaaatgca gcgagatttt catctcgggc catttttccc gcacggccgc gatgactcgc
     1261 tcgatacgga ccggcgtggc ccaccccatc gtgtcggcga gcgaaaagag ttcgatcttc
     1321 gcgccggtct catcggcgat cttcataccg tcgtcgaggg ttttcaccac cgtcgcgggg
     1381 gagatatcgc cctggtagtt gcagccgaac gcggccatta cgccgacgcg gctgaccggg
     1441 atgcccttcg ccaaatgcgt ttccgcgtgg cgcttcattt gcgcgagatt ttcctcaggc
     1501 gtgcggttga ggttcttttt cgtgaaccca gccgacgccg tcagcgagat cgagccgctg
     1561 atggtcaact tgtccttgaa cgccagcgcg cggtcgaggc cgttgccgtt gaaataaagt
     1621 ccggtgtagt ggacgccggg cttcgcggtg aacccggcgg cgactgcctc ggcgtcggcc
     1681 cacccgggaa ccagccgcgg gttaacgaag gaacagacct gaatgtgatg caggccggtt
     1741 tccgacaatg cgtcgatcag ctttatcttg tcggcggtgc tgatcggacc gggctcgatc
     1801 tgaaatccct cgcgtggacc ttcctcgtgg atttcgcagt gttttggcag gtcggacatg
     1861 gtctgatcct ttcagatgac tttttcggat ttgaatccgg cgatgtcggc tttgctgtag
     1921 ccaagctcgg tcagaatttc gtcgttgtgc tggccgaaca tcggcggcgg acgatccacc
     1981 tgcgcgccgc catgggcaaa tttgaacgcc gcgaccggga cgctgaacga accggagatg
     2041 ccgggcgcgc tttcgtgctt gtgcagaatg ttccggctgc tcagctgcgg gtcggccaat
     2101 gcctcgccca tcgtgcgcac gcgggacgcg ggaacgtggc gttcctgcaa ccattcttcc
     2161 cattcggcgg cggtgcgggt tagcatgatc tcggccagca ccttcgcttc gcggtcatga
     2221 tcggcatgac gctcgtcgtt ggtgcgcttg atcatctcgg gtcgctccag cacggtccac
     2281 agccgcttct gctgacgcag gttcgaagcg ccgatcatga gcatgccttc tttcgtcaca
     2341 taggcctggc tggtggcgtg acccgttgtg ttgccggacg gtt
//
LOCUS       DATIMH010000952         1744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3976630, whole genome shotgun sequence.
ACCESSION   DATIMH010000952 DATIMH010000000
VERSION     DATIMH010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3976630"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..963
                     /locus_tag="VLI90_16495"
     CDS             109..963
                     /locus_tag="VLI90_16495"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSV15864.1"
                     /translation="MALSDHDLAMKSVAYRRSILRWIRHAGAGHTGGSLSCVDILNVL
                     YNRVLRRVAPATFGDPDRDRYIQSKGHSVEALYVVLADRGFFAEEKLATLCGYDSPFV
                     GHPTRAVPGIEQNTGALGHGLPIGAGTAIAGKKDRRDFRVYVLMGDGELAEGSNWEAA
                     MAAAHYRLDNLVAIVDHNMLQISGRTRDVCSNEPLDEKFAAFGWDVRIADGHNFRQLT
                     EALLPPVNTGKPRVVIANTVKGKDISFMQDVAKWHHGVPSGSEYAAAVRELDALEARL
                     AKAIGGAA"
     gene            960..>1744
                     /locus_tag="VLI90_16500"
     CDS             960..>1744
                     /locus_tag="VLI90_16500"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSV15865.1"
                     /translation="MSAPAPSMYSPDAKAMAERLGLKMGRANLEAFAETLLEMAKADR
                     NVLAVTSDSRGSGKLGPFAQALPDQLIELGIAEQNLVGVAAGLASAGKSVYAVSPACF
                     LTARALEQIKNDVAYSENPVKVIGISAGVSYGALGSTHHSLHDLAALRAINNIDVVVP
                     ADNFEAGQVIRQAAHRPTPVYVRFGKAPLYDLHAPSTQWELGRSITLRDGSDVAFIAT
                     GETVVHALLAAGTLAADGITARVISMHTVKPLDTQAVLKAARE"
BASE COUNT          313 a          523 c          604 g          304 t
ORIGIN      
        1 tttcgagcat cacgtggccg tcaatgcatc gcacaccgct gatgtgctga tggaggcgtg
       61 cgggaattac ctgggctgga acatgcaccg ccacgggaac taaacgcgat ggcactaagc
      121 gatcacgatc tggcgatgaa gtcggtagcg tatcggcgca gcatcctgcg ctggattcgc
      181 cacgctggcg ccggtcatac cggcggtagc ctgtcgtgcg tcgatatcct gaacgtgctg
      241 tataaccgtg tgctccgtcg cgtcgcgccg gcgacgttcg gcgatcccga tcgcgatcgg
      301 tacattcaat ccaagggaca ttccgtcgaa gcgctgtatg tcgtgctcgc cgaccgcggg
      361 ttctttgcgg aagaaaagct ggcgacgctg tgcgggtacg attcgccgtt cgtcggtcat
      421 ccgacgcgtg cggtgccggg gatcgagcag aacaccggcg cgctcggcca cgggttgccg
      481 atcggcgcgg gcacggccat cgcggggaag aaggaccgcc gcgatttccg cgtgtatgtg
      541 ctgatgggcg atggcgaact ggctgaggga tcgaactggg aagccgccat ggcggcagcg
      601 cattatcggc tcgacaacct ggtggcgatc gtcgatcaca acatgctgca gatttccggc
      661 cgcacgcgag atgtgtgcag caatgagccg ctcgatgaga agttcgcggc gttcggttgg
      721 gacgtgcgca tcgcagatgg tcataatttc cgccagctga ccgaagcgct cctgccgccg
      781 gtgaacacgg gcaagccgcg cgttgtgatc gccaatacgg tcaagggcaa agacatcagc
      841 ttcatgcagg acgtcgccaa gtggcaccat ggcgtgccga gcggatccga atacgccgcc
      901 gcggtgcgcg agttggatgc gctggaagcg cgactggcga aggcgatcgg cggtgccgca
      961 tgagcgcgcc ggcaccatcg atgtattcgc ccgacgccaa ggcgatggcg gaacggcttg
     1021 gcctgaagat gggccgggcg aatctggagg cgtttgccga gacgctgctg gagatggcca
     1081 aggccgaccg caacgtgctg gcggtgacga gcgattcgcg cgggtccggc aagctcgggc
     1141 cgttcgcgca ggcgctgccg gatcaactga tcgagttggg cattgcggag cagaatttgg
     1201 tcggcgtcgc cgcgggattg gcctcggcgg ggaaaagcgt ctacgccgtc tcgcccgcgt
     1261 gcttcctcac ggcccgcgcg ctcgagcaga tcaagaacga cgtcgcgtac tccgaaaatc
     1321 ccgtcaaagt gatcggcatt tccgccggcg tcagttatgg agcgctcggt tcgacgcatc
     1381 actcgctgca cgatctggca gcgctgcgcg cgatcaacaa catcgacgtc gtcgtccccg
     1441 ccgacaactt tgaagcgggg caggtgattc gacaggcggc gcatcggccg acgccggtgt
     1501 acgtgcgctt cggcaaggcg ccgctttacg atctgcatgc gccgagcacg cagtgggagc
     1561 ttggtcgatc aatcacgctg cgcgatggat cggacgtcgc gttcatcgcg accggcgaaa
     1621 ccgtcgttca cgcgctgctc gccgccggga cgctggcggc ggatgggatc accgcgcgcg
     1681 tcatctcgat gcacacggtg aagccgctcg acacgcaggc ggtgctgaaa gcggcccgcg
     1741 agtg
//
LOCUS       DATIMH010000953         5092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1095839, whole genome shotgun sequence.
ACCESSION   DATIMH010000953 DATIMH010000000
VERSION     DATIMH010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095839"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1362
                     /gene="tyrS"
                     /locus_tag="VLI90_16505"
     CDS             157..1362
                     /gene="tyrS"
                     /locus_tag="VLI90_16505"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSV15866.1"
                     /translation="MSLTIDQQIAALSRRCERVLSEQDLRAKLERSAKTGSPLRIKLG
                     MDPTAPDVTLGHAVPLSVVRQFQDWGHKAVIIIGNYTARVGDPTGRNKTRPPLSGEEI
                     DRNAETYVAQVGKILRTDPEHLEVRHNGEWLAKMDLVDIIRLASRKTVAQVLTREDFA
                     KRYAEGVDIRLHEILYPLLQGWDSVCVDADVEMGGSDQLFNNLVGREFQKEEGKDGQV
                     VIVTPLLVGTDGTDKMSKSKGNYIAVTDPPGGDGGMFGKVMKLPDALMESYYTLLTDL
                     SPEEFKPLIASNPRDAKVRLAKHLISWLHEPAAGDAAERGFMAATHGQIPDEIPEVSL
                     ESPGPHKLAPLLVKAGMVSSNGEGIRKMKEGAVRIDGDRVTDVQKEYTFDKPVVLQLG
                     SRKFARLKP"
     gene            1421..1591
                     /locus_tag="VLI90_16510"
     CDS             1421..1591
                     /locus_tag="VLI90_16510"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSV15867.1"
                     /translation="MGSEDRAAGKMKQAKGKANEVMGAAKGDTGQEIKGKAQKAVGKV
                     QDKLGKASSGNR"
     gene            complement(1729..2166)
                     /locus_tag="VLI90_16515"
     CDS             complement(1729..2166)
                     /locus_tag="VLI90_16515"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSV15868.1"
                     /translation="MPTANDILGHKGFSVHVVKPSTSVLDAVERMNQLRVGAVVVMDD
                     GEVKGMFTERDVLMRVVGEGRDPAGVKVGDVMTRDVICVTPDTDMDEISAIMKNRRIR
                     HLPVCDDDGDLLGLISIGDVNAYHASNQEMTITYLNEYIYGRA"
     gene            2370..2711
                     /locus_tag="VLI90_16520"
     CDS             2370..2711
                     /locus_tag="VLI90_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15869.1"
                     /translation="MSDLISRALLCGLGLASLTKDAIQKTVEDLVNQSKISEAEGRKL
                     VKEVQRRSEKARKSLEKNVNSAVHSVFEHMDLNAVISDRLKGSKPHAKRSAKTRRPRR
                     AAATKRKSAAH"
     gene            2816..4474
                     /locus_tag="VLI90_16525"
     CDS             2816..4474
                     /locus_tag="VLI90_16525"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HSV15870.1"
                     /translation="MHLQSAAQIPGDVARASEVARVLIKYGLAQWLKGTEWEPARRML
                     TSHTGEVLTDQTFAVRMRMALTDLGTTFIKLGQVLGTRPDLVGPDVARELSHLQSGTP
                     PDPGDVAIATIERELGRPIAECFAEFDHTAMASASIGQVHRAKLPNGRAVVVKVQHPG
                     IESTIRRDLNILSTFAGMAEHQEEFRRYQPTAVVREFRQTLMRELDFRREMRSLQQFR
                     RNFADDQTVTFPKPYPELSSGRVLTMQLLKGTSVGDIVNLQHSRIDSVELARHGAGVF
                     VQMIFRDGFYHADPHPGNILAMSKGRVGILDCGMVGRIDDDLRERIIEILLAAADRDA
                     PRLADVIAITCKAPSNLDRGGLSADLMEVFGQYGSQAVDQFDIGGALTAVTRIMHEYN
                     LFMPSRLSMLIKCLIVLEGTGKGLNANFNLAELLEPYRRQFVLQQLSPENWLRKAKRV
                     KRDWEMLANSIPRGFNNLLDQLQSGHFAVRVKHPPLETSVNRMVYGLCTSALLLASAL
                     LWVHNVPPTIRGISVLGASGYLLAFFLAARIFWLIRRAERRKDD"
     gene            4626..4838
                     /locus_tag="VLI90_16530"
     CDS             4626..4838
                     /locus_tag="VLI90_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15871.1"
                     /translation="MAQVTFDEVLEAIEHLPVDEQADLLEVVRRRLAERGRRRVIDDI
                     AEARDQFSSGAAKPASVEEIMREIDS"
BASE COUNT         1036 a         1527 c         1642 g          887 t
ORIGIN      
        1 ccgatgggtg tcgaacgatc ctcgaactcc acggtccgcg ggctaaagcg ccgcggctgc
       61 gatggaatcg tctgcgaagg gaattgtccc ggaattgtcc ccgattgccc gcgttcttcc
      121 gcctccttac aattcccgcc ccactgatag aggcttatgt cactgaccat cgaccaacag
      181 atcgccgcgt tgtctcgccg atgtgagcgg gttttgagcg agcaggacct gcgcgcgaag
      241 ctcgagcgca gcgccaagac cggctcgccg ctgcggatca agctggggat ggacccgacg
      301 gcgccggatg tcacgctcgg ccacgcggtg ccgctgagcg ttgtgcggca gttccaggac
      361 tggggacaca aggcggtcat catcatcggg aactacacgg ctcgcgtggg cgatccgacg
      421 gggcgaaata agacgcgccc gccgctgagt ggcgaggaga tcgatcgaaa cgccgagacg
      481 tacgtcgcgc aggtggggaa gatccttcgc acggatcccg agcacctgga ggtccgccac
      541 aacggcgaat ggctcgcgaa gatggacctg gtggacatca tccgcctggc atcgcgcaag
      601 acggtggcgc aggtgctgac gcgggaggac ttcgccaagc gctacgccga gggcgtagac
      661 attcggctgc acgagattct ctacccgctg ctgcagggat gggactcggt ttgcgtggat
      721 gccgacgtcg agatgggcgg cagcgatcag ctgttcaaca acctggtcgg ccgcgaattc
      781 cagaaggaag aggggaagga cggccaggtc gtcatcgtga cgccattgct ggttggcacc
      841 gacggcaccg acaagatgtc caagagcaag ggcaactaca tcgcggtgac ggacccaccg
      901 ggcggcgatg gcgggatgtt cggcaaggtg atgaagctgc cggatgcgct gatggaatcg
      961 tactacacgc tgctgacgga cctgtcgccg gaggagttca aaccgctgat cgcaagcaat
     1021 ccgcgcgacg cgaaggtgcg attggcgaag catctgatca gctggctgca cgaacccgcc
     1081 gcgggggatg cggcggaacg cgggttcatg gcggcgacgc acgggcagat tccggatgaa
     1141 atcccggagg tgtcgctcga atcgccgggt ccgcacaagc tggccccgct gctggtgaaa
     1201 gccggcatgg tttcgtcgaa cggcgaaggc atccggaaga tgaaggaggg tgccgtccgc
     1261 atcgatggcg atcgcgtcac ggacgtccag aaggaataca cgttcgacaa gcccgttgtg
     1321 cttcagctcg gcagccgaaa gttcgcccgc ctcaagccat gaagttggct cggcatcatc
     1381 tttgctttgg tcccccgcat ctcttaccac ggaggtcctg atgggatccg aagatcgcgc
     1441 tgcgggaaag atgaagcagg ccaagggcaa ggcgaacgaa gtgatgggcg ccgccaaggg
     1501 cgacaccggc caggagatca agggcaaagc gcagaaggcc gtcggcaagg tgcaggacaa
     1561 gctcggcaaa gcgtcgagcg gcaaccgctg aggtcgagct tcagcataag taagaccccg
     1621 caaggcccac ggctttatcc gacagttcaa cgcaaagagg ccggtcatcc cgcggaggaa
     1681 tgaccggcct caattgtttt tgagttcgac cgcgagttcg gcgctcgctc acgcccggcc
     1741 gtagatgtat tcgttcaagt aagtgatcgt catttcctgg ttcgacgcgt ggtacgcgtt
     1801 cacgtcgccg atcgagatca atcccaatag gtccccgtca tcatcgcaca ccggcaggtg
     1861 gcggatccgg cggttcttca tgatcgcgct gatttcgtcc atgtcggtat cgggcgtcac
     1921 gcagatgacg tcgcgggtca tcacgtcgcc gaccttcacg cctgcgggat ctcggccttc
     1981 gccgacgacg cgcatcagca cgtcgcgctc ggtgaacata cctttgactt cgccgtcgtc
     2041 catcaccacc accgcgccga cgcgcagctg gttcattcgc tcaacagcat cgagaacgct
     2101 ggtggacggt ttgacgacgt gaacgctgaa ccccttgtgg ccaaggatgt catttgcggt
     2161 gggcatgcga cggctcctgt ggttagcgga cctctcgaac tggtgtgcgc cgaatagaac
     2221 agcgcacgga cgtggcgagt aaactgagcg aattatccct tgatgcaaca aagtgtgcaa
     2281 catcgttctt taattcacga gggattttcc tggccctgaa gtacaatcga accgggcaat
     2341 gacgaaggca aaatcgaaag gacattttta tgagtgatct gatcagcaga gcgctcctct
     2401 gcgggctggg actggccagc ctgacgaagg atgcgattca gaagacggtc gaggacttgg
     2461 tcaaccaatc gaagatctcc gaagccgaag gccgaaagct ggtgaaggaa gtgcagcggc
     2521 gatcggagaa ggcgaggaaa tcgctggaga agaatgtcaa ttcggccgtg cactcggtgt
     2581 tcgagcacat ggatctgaac gcggtcatca gtgaccggtt gaaaggcagc aaaccgcacg
     2641 cgaagcgctc ggcaaagacg cggcgaccca ggcgcgcggc tgcgacgaag aggaagtccg
     2701 ccgcgcactg aggagagcgg gcccgtcctc gggccggacc gggccgggga cggcccggct
     2761 ctgctgggtc tctccctgat gctgacgcag ccgcgtctca cgcaactcgc agcccatgca
     2821 ccttcaatcg gccgcccaaa tcccgggaga tgtcgctcgc gcctctgagg tggcgcgggt
     2881 gctcatcaag tatggcctcg cccaatggct caaaggcacc gagtgggaac cggcccggcg
     2941 gatgctcacg agccacaccg gggaggtcct caccgatcag acgtttgccg tccgcatgcg
     3001 gatggcgctg accgacctgg ggaccacgtt catcaagctg gggcaggtgc tggggacgcg
     3061 gccggatctg gtcgggccag acgtcgcgcg cgagctgtcg cacctgcaga gcggcacacc
     3121 gcccgatccg ggggacgtcg cgatcgcgac gatcgaacgg gagctgggcc ggccgatcgc
     3181 cgagtgcttc gcggagttcg atcacaccgc gatggcgtcg gcgtcgatcg ggcaggtcca
     3241 ccgcgccaag ctgcccaatg gtcgggcggt cgtcgtaaag gtgcagcatc ccggcatcga
     3301 aagcacgatc cgacgggacc tgaacatcct gtccacgttc gccggcatgg ccgagcacca
     3361 ggaagagttt cggcgttatc aaccgacggc cgtggtgcgc gagttccgcc agacgctgat
     3421 gcgcgagttg gatttccgcc gcgagatgcg cagcctgcag cagttccgcc gcaacttcgc
     3481 cgacgatcag accgtcacgt tcccaaagcc ttatcccgaa ttgagcagcg gccgtgtgct
     3541 gacgatgcag ttgctcaaag gtacgagcgt cggcgacatc gtcaacctac agcacagccg
     3601 gatcgacagc gtcgagttgg cccgccacgg cgcgggcgtg ttcgtgcaga tgatcttccg
     3661 cgacggcttc tatcacgccg acccgcatcc gggaaacatc cttgcgatga gcaagggccg
     3721 ggtgggcatt ctcgattgcg ggatggtcgg gcggatcgac gatgacctgc gcgagcggat
     3781 catcgagatt ctcctggccg ccgccgaccg cgatgcgccg cggctggcgg atgtgattgc
     3841 catcacctgc aaagcgccgt cgaacctgga ccgcgggggg ttgagcgcgg atttgatgga
     3901 agtcttcggc caatacggct cgcaagccgt cgatcaattc gacatcggcg gcgcgttaac
     3961 ggccgtaacg cgcatcatgc acgagtacaa cctgttcatg cccagccggc tgtcgatgct
     4021 gatcaagtgc ctgatcgtgc tggaaggcac cggcaagggt ctcaatgcaa acttcaacct
     4081 cgcggagttg ctagagccct accgccggca gttcgtgctg cagcagctgt cgccggaaaa
     4141 ctggctgcga aaagcgaagc gcgtgaagcg cgactgggag atgctggcca actcgatccc
     4201 gcgcggcttc aacaacctgc tcgaccagct ccaatccggc cacttcgccg tccgcgtcaa
     4261 acacccgccg ctggagacgt cggtcaaccg catggtctac ggcctgtgca ccagcgcgct
     4321 gctgctggcg tcagcgctgc tgtgggtcca taacgtcccg ccaaccatcc gcggcatctc
     4381 cgtcctcggc gcgtcgggat atcttctcgc gttcttcctc gcggccagga tcttttggct
     4441 aatccggcga gcggagcggc ggaaggatga ctgacactcc gaagcacttg ctcgcaaagt
     4501 ccatgtagct gcgacgcctg cgtcgccttc gcgcaaaggc gacgcaggcg tcgcaactac
     4561 agttcacatt agacctgata aacgaaccgc agaattgata tgctattcag cgaaaggtag
     4621 gccgcatggc acaagtgaca ttcgacgagg tccttgaggc aatcgagcat ttgcccgtcg
     4681 atgaacaagc tgatttgctc gaggtcgtgc ggcgtcggct cgcggagcgg gggcgacgcc
     4741 gcgtgatcga tgacatcgct gaagcgcgcg atcagttcag cagcggcgca gcaaaaccgg
     4801 caagtgtcga agagatcatg cgcgagatcg actcgtgaga cgcacgctga ttcagtcgcc
     4861 tgcgttcatt cgcgcggcca agcgctttgc agagaaacag tccacagcgg cagaagcgat
     4921 tcgccaaaca ctcgttcgac ttcaggaaga tgccctggat ccgagcttga gaacgcacaa
     4981 cgctcaaagg tgatttagcc gactgttggg cctccagtgc cggctatgat ttgcgcatcg
     5041 tcttccagtt cgtgcagcac cgcggcgccg aaggaattct gctgctttcg gt
//
LOCUS       DATIMH010000954         4769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_808107, whole genome shotgun sequence.
ACCESSION   DATIMH010000954 DATIMH010000000
VERSION     DATIMH010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808107"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VLI90_16535"
     CDS             complement(<1..658)
                     /locus_tag="VLI90_16535"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HSV15872.1"
                     /translation="MLASSVYRGVVVPMVSPFTPAGRIDEPAVGRIVAHLLKGGVAGI
                     FPLGTTGESASIRREDKRQLVSATVHAVHGRAMVYAGIAGNCFAESIDAAAEYRQLGA
                     DAVVAHVPSYYPLNDCEIETYFERLADQVPLPLVLYNIPVTTHHHIALDVVDRLRNHP
                     NIVALKDSANDGHRLTELLHRTGGRGGWPVLVGTSAQFTHGLKLGAVGLVPSGGHLVP
                     D"
     gene            complement(659..1822)
                     /locus_tag="VLI90_16540"
     CDS             complement(659..1822)
                     /locus_tag="VLI90_16540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HSV15873.1"
                     /translation="MNPTANIIALHQPRRLAFGAGCAARAIDDLRERHCQRVLLVASR
                     STRRFCAPFQSAFATAGSSILVWDKIDAEPTVETFRQAMTAARDFRPDAVVGIGGGSV
                     LDIAKLVAAMLGSDLDIRDAFGIGKISGRSIHLACLPTTAGTGSEVSPNAILLDEADQ
                     LKKGVISPHLMPDAAYVDPALTLSVPPAVTSATGMDALTHCIEAYANRFAHPLIDLYA
                     LQGIRLIAQSLEQAVRHGDDLAARMIVALGSMYGGMCLGPVNTGAVHALAYPLGGEFH
                     IAHGVSNALLLPHVLAFNLPAAPQRYAQIAIAMGEPMASGASDLDTAARGVARVADLS
                     RRCGVPQRLSQIGIPESAIERMAKAAMTVTRLLERNVRQVTLNDAIAIYRSAF"
     gene            complement(1914..3911)
                     /locus_tag="VLI90_16545"
     CDS             complement(1914..3911)
                     /locus_tag="VLI90_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15874.1"
                     /translation="MMIELNGFVLQLSLSAALHGQWISLPIAAHGAGGFAVEHKGIRA
                     QLHLTPERDRLRYQLSFSSTTKTRLRFRAELLGQRDLFHLIPGNIHGDNNAAHVRPGE
                     FPCLTSARPAERNCSPLWEFRADRASHPVSTLCSQAGAAGISIDPYSPSDQADDGFIR
                     NGVFAELPNAFGVALGYGNDPLTFVQKTQFTAATCDIVHEAAASGFIFAVPGDGRLAA
                     HDIIRALHQQLRDIPMYSHSYEQALRALADAFAQVSWSNELQQYTNRKCRVPVDRELQ
                     PWRAIVEIGWTGGSMLAYPFTRAERIFSNLKLPKTGGRIFDEICTGFNAGSGFINDAA
                     LNRFTQNIPDGWNTSQINGWWSGFLPQTLNNHCAYNNAHAAYYLLRGATANDRQWIKT
                     ALAVLDTAVELQRADGAFGYIFSSTERKVIDWDGFAGCWFAPALVLAWRLTGSRRYLE
                     SASRAMRYYWPFVRALTCWGSPMDTYKSIDSEGNLAFIRGARLMHEFTGDAEYLTMLR
                     DGAHYEYLWRYGFRTRPQCPPLKGSAWNSCGGSLTSVSNAHIHPMSVVATEDLKYLAK
                     QASDNYHDDRATDGIAWLMNTMELYPDTVGYGRYGVLSERTCPSDGLLAETYADDGSP
                     ASTWWSYNAWAAGSAMEAIAERVLAQARESGPLSHVPVGEG"
     gene            4132..>4769
                     /locus_tag="VLI90_16550"
     CDS             4132..>4769
                     /locus_tag="VLI90_16550"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HSV15875.1"
                     /translation="MRERLSLDRNWRFALGHAANAALDFEFARSRCLVKAGEGRGAAG
                     PKFDDSQWRLVNLPHDWASELPLDPKGDKELGEHGFRAIGPDHPEHSVGWYRRAFDIP
                     ASDLGRRISVEFDGVFRDSVVWINGHRLDRHASGYTPFRYDLTDMINYGGANVLSVRV
                     DASSWEGWWYEGAGIYRHVWLVKTAPLHVAPNGTVVTSKLSKAGANVTVRTTV"
BASE COUNT          815 a         1434 c         1703 g          817 t
ORIGIN      
        1 gatcgggcac gagatgtccg ccgctgggga cgagccccac cgcgccgagc ttcagaccat
       61 gcgtgaactg cgcgctcgtg ccgacgagaa cgggccagcc gccgcgcccg ccggtgcgat
      121 gaagcagctc ggtcaggcga tgcccgtcat tcgcggaatc tttcagcgcc acgatgttcg
      181 gatgatttct caggcgatcg acgacatcga gcgcgatgtg gtgatgcgtc gtcaccggga
      241 tgttgtacag caccagcggc agcggcacct ggtcggccag ccgttcgaaa tacgtttcga
      301 tctcgcaatc gttcagcgga tagtacgacg gaacgtgcgc gacgacggca tccgcgccca
      361 actgccgata ctccgccgcg gcgtcgatcg actcggcgaa gcaattgccg gcgatgcccg
      421 cgtacaccat cgcgcggccg tgcaccgcgt gcaccgtggc cgacaccagt tggcgcttgt
      481 cttcccggcg gatcgacgcc gactcgccag ttgttcccag cgggaagatt ccggcgacgc
      541 cgcccttgag cagatgcgcg acgatgcgcc cgaccgccgg ttcgtcgatg cgacccgcgg
      601 gggtgaaggg gctcaccatc ggcacgacca cgccacgata gactgagctg gcaagcatct
      661 aaaacgcgct ccggtaaatg gcgatcgcat cgttcagcgt cacctggcga acgtttcgct
      721 cgagcaatcg agtgaccgtc atcgcagcct tggccatgcg ctcgatggcc gattcgggga
      781 ttccgatttg agataggcgt tgcggcacgc cgcatcggcg ggacaagtcg gcgacgcgcg
      841 cgacgccgcg cgccgcggtg tcgagatcgc tcgcgccgct ggccattggt tcacccatgg
      901 cgatggcgat ctgcgcatag cgctgcggcg ccgcgggaag attgaacgcc agcacgtggg
      961 gcagcagcag ggcgttggac acgccgtggg cgatgtggaa ttcgccgccc agtggatacg
     1021 ccagcgcgtg caccgcgccc gtgttgaccg gaccgaggca catgccgccg tacatactcc
     1081 cgagcgcgac gatcattcgc gcggccagat cgtcgccatg ccgcaccgcc tgctccagcg
     1141 actgcgcgat caagcgaatg ccttgcagcg cgtagaggtc gatcaacgga tgcgcgaagc
     1201 ggttggcgta cgcttcgatg caatgcgtga gcgcatccat gccggtggcc gaggtgacgg
     1261 cgggggggac gctcagcgtg agcgcgggat cgacgtacgc cgcgtccggc atcaggtgcg
     1321 ggctgatcac gcccttcttc agttggtcgg cctcgtccag caggatggcg ttgggtgaga
     1381 cttcgctgcc ggtgcccgcg gtggtgggca ggcaggcgag gtggatcgat cgaccggaaa
     1441 ttttgccgat gccgaaggca tcacggatgt cgaggtccga gccgagcatg gcggcgacga
     1501 gtttcgcgat gtcaagcacg ctgccgccgc cgatgccgac gaccgcgtcg gggcggaagt
     1561 cgcgggcggc ggtcatggct tggcggaagg tctccaccgt tggttcggcg tcgatcttgt
     1621 cccagaccaa gatggatgag ccggcggtgg cgaaggcgga ttggaacggc gcgcagaatc
     1681 gccgcgtcga gcgcgaggcg acgagcagca cgcgctggca gtggcgttcg cgcaggtcgt
     1741 cgatggcgcg cgcggcgcag ccggcgccga aggcaagccg gcgcggctgg tgcagggcga
     1801 tgatgttcgc agttgggttc attcttctcc ccctcgcccg gtactccggg agagggcagg
     1861 ggtgagggtg atttcgaacg tcgagcggcc ctcgaaatta aatcaccctc accctaaccc
     1921 tctcccacgg gtacgtggga gaggggacca gattcgcgcg cctgggctaa aactcgttct
     1981 gcgatcgcct ccatcgcgct gccggcggcc caggcgttgt agctccacca cgtgctggcg
     2041 ggcgatccgt cgtccgcgta cgtctcggcg agcaagccgt cgctggggca ggtgcgttcg
     2101 gacagcacgc catatcgtcc gtagccgacc gtgtcgggat acagttccat cgtgttcatc
     2161 agccaggcga tcccgtcggt agcgcgatcg tcgtgataat tatcactcgc ctgcttcgcc
     2221 aggtatttca ggtcttcggt ggcgacgacg ctcatcgggt ggatgtgcgc gttggagacg
     2281 gaggtgagcg atccgccgca ggagttccaa gcgctgccct tgagtggcgg gcactgcgga
     2341 cgggtgcgga agccgtatcg ccagaggtat tcgtaatgcg cgccgtcgcg cagcatggtc
     2401 aggtattcgg cgtcgccggt gaattcgtgc atcagccgag cgccgcggat gaaggcgagg
     2461 ttgccttcgg aatcgatgct cttgtacgtg tccatcggcg aaccccagca ggtgagcgcg
     2521 cgaacgaacg gccagtaata gcgcatcgcg cggctggcgg attcgagata ccgccggctg
     2581 ccggtgaggc gccacgcgag caccagcgcc ggcgcgaacc agcaaccggc gaagccgtcc
     2641 cagtcgatca ctttgcgctc ggtgctgctg aagatgtaac cgaacgcgcc gtcagcgcgc
     2701 tgcagctcga ccgccgtgtc gagcaccgcc agcgcggtct tgatccactg tcgatcgttc
     2761 gcggtcgcgc cacggagcaa gtaatacgcc gcgtgggcgt tgttgtaggc gcagtgattg
     2821 ttcagcgtct gcggcaaaaa tccggaccac cagccgttga tctgcgaggt gttccagcca
     2881 tcagggatgt tctgcgtgaa gcggttgagt gccgcgtcgt tgatgaaccc ggagcccgcg
     2941 ttgaagccgg tgcagatctc gtcgaagatc cggccgccgg tcttgggcag cttcaggttg
     3001 gagaagatgc gctcggcccg cgtgaacgga tacgccagca tgctgccgcc ggtccaaccg
     3061 atttcgacga tcgcgcgcca cggttgaagc tcgcgatcga ccggcacgcg gcacttgcgg
     3121 ttggtgtatt gctgaagctc gttcgaccag ctgacctgcg cgaacgcatc ggcgagggcg
     3181 cgcagcgcct gctcgtacga atgcgagtac atcgggatat cgcgaagctg ctggtgcagc
     3241 gcgcggatga tgtcgtgtgc cgccaatcgt ccatcgcccg ggacggcgaa gatgaaccca
     3301 cttgccgcgg cttcgtggac gatgtcgcat gtggcggcgg tgaactgcgt cttctgcacg
     3361 aacgtgagcg gatcgttgcc gtaaccgagc gcgacgccga aggcgttggg cagctcggcg
     3421 aagacgccgt tgcggatgaa gccgtcgtcg gcctggtccg atggcgagta gggatcgatg
     3481 gaaatgccgg cggcgccggc ttgcgagcag agggtcgaca cggggtgcga agcgcggtca
     3541 gcgcggaatt cccagagcgg cgagcagttt cgctccgccg ggcgagcgct ggtgaggcac
     3601 gggaattcgc cgggccgcac gtgggcggcg ttgttgtcgc cgtggatgtt gccggggatc
     3661 aggtgaaaca ggtcgcgttg gccgagcaac tcggcgcgga agcgcaggcg cgtcttcgtc
     3721 gttgaggaga agctcagttg atagcgcagc cggtcgcgct cgggcgtcag gtgcaactgc
     3781 gcgcggatgc ccttatgctc gacggcgaag ccgccggcgc cgtgggcggc gatgggcagc
     3841 gagatccact gcccatgcaa cgccgccgac agcgacagct gcaggacgaa accgttcaac
     3901 tcgatcatca tggtgcgcac ggcaacgtaa tcgcagcgtc gcgcgggggg caatgtaatt
     3961 cggcgtaata gttttgcact tttgcgcagg ggggtttgaa aacgttcctc gggcgtggga
     4021 aatatcgagc aaaagcccac ggctctgccg tgggacgggc gttgagaaag cggtcccacg
     4081 gcatagccgt gggcttttgg gcgcgagctt ttggcgcgga gtgtgaagcg catgcgagaa
     4141 cgtctgtccc tcgatcgcaa ctggcgcttc gcgctggggc acgcggctaa cgcggcgctg
     4201 gacttcgagt tcgcgcgcag ccgatgtctc gtcaaggccg gcgaaggccg cggggcggcc
     4261 ggtccgaagt tcgatgacag ccagtggcgg ctcgtcaatc tgccgcacga ctgggcgagc
     4321 gagctgccgc tcgatccgaa gggggacaag gagctcggcg agcacggctt tcgcgccatc
     4381 ggccccgacc atcccgagca cagcgtcggg tggtatcgcc gtgcgttcga tattcccgcc
     4441 tcggatctgg gccggcggat cagcgtggaa ttcgacggcg tgtttcgcga cagcgttgtg
     4501 tggatcaacg gccaccggct ggaccggcac gccagcgggt acacgccgtt tcgttacgac
     4561 ctgactgaca tgatcaacta cggcggggcg aacgtgctga gcgtgcgcgt ggacgcgagc
     4621 agttgggaag ggtggtggta cgaaggggcg gggatttatc ggcatgtgtg gctggtgaag
     4681 accgcgccgc tgcatgtggc gccaaatgga acggtggtca cgagcaagtt gagcaaagcc
     4741 ggcgcgaacg tgacggtgcg gacgacggt
//
LOCUS       DATIMH010000955         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5717041, whole genome shotgun sequence.
ACCESSION   DATIMH010000955 DATIMH010000000
VERSION     DATIMH010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5717041"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..450
                     /locus_tag="VLI90_16555"
     CDS             145..450
                     /locus_tag="VLI90_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15876.1"
                     /translation="MHRWLHWLFFGLTAASIASVLMSVAWWVQSYREGFAGGGIMANV
                     GNFVLIFYHGEYRYEDAQTFIHFGFWQPALASTLSSVLFGRLVHRMYACSDAIAGKK"
     gene            620..3103
                     /locus_tag="VLI90_16560"
     CDS             620..3103
                     /locus_tag="VLI90_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15877.1"
                     /translation="MRSFLAGFFLLLLMPAFALAQAKGEVESIGFNNSYRPDAWTPMV
                     VRLRPETNEPGTYQIQVWQHDIDGDRPVFTRQITLNGADQAREQRFWMYFLPQPIDKG
                     LPDPTNGGTLKDLQRELPVFLCTASGKQITQLPITSTLLNVDPVRDYAQQPRSAKLLL
                     AISDGTSTPAWRDYESALGLVEDVVVVNLQAKDLPEDPIGYEGVDAIVWLNADPADLD
                     RGGAHKLATLQDYVRSGGHLVVTQPTTDWQKILGFADMLPVTVLGVEDKNDLEPLRSM
                     AKPLDPIEALKPRVDQWLRPVGPFQFAKAKAKSRAVVEAWIDWHGDGSYTDATPYIAR
                     SPYGLGAVTWVAQDLGNPAIAGKTANGAGWPYVWDKVFGWKNDTYVPPPGVSKDNEDL
                     KPRMDLYRPGSPIDLGYPLTQGLNLDARGAFLIFIAVAFFFVYLIVAGPGSYLYLASK
                     KRARLSWFFFGGAAIAATLLTVAVVKAVMHYPPQLRHLTVVRVAPGQPTYIYSRFGLY
                     ITHDGDQSIELTGTAHDGVSYLSGYAEHPQQLGDVTEFPAPADYTVPVRDIASIDPPA
                     LTVPYRSSLKKFQAKWIGDLSQQVGKFTASNVKMTVEDGRTLLSGAITNQTNNDFNDV
                     YIAYHGQSNRDEDRLIYIPLWKRGVTLDLQKDLAKAPVVGQLGNTNLAAIPGDGKIIS
                     DEIGTSNVKSGTARDMHWIGFWYRKFYNSQYHDANTEDPRYTYVYPMLSLFDRLPPAW
                     NKPDVASTGVGSTWDRFELYRRGGRMLNVSQSLMAGQLAILASAQGPLPMPMEINGDK
                     AEGQGTTFYQFLLPIDRGDVDKPTTQPAG"
BASE COUNT          611 a         1002 c          917 g          576 t
ORIGIN      
        1 gtgggctgaa atatcgctcg cattcaaaga ttttcactct tccctccaac agcgcttcgc
       61 atccccctcc ccccagacca ccttttcggc ccccaatcct tgacatctac aaccgcctgg
      121 cgggacactc tgtagagtac ggttgtgcac cgctggcttc actggctttt tttcggcctc
      181 accgccgcct ccatagcatc ggtgctcatg agcgttgcgt ggtgggttca aagctaccga
      241 gaaggcttcg ccggcggcgg tatcatggcc aatgtaggga acttcgtcct catcttctat
      301 cacggcgaat atcgctacga agatgcccaa acatttatcc acttcggctt ctggcaaccg
      361 gcgctggcaa gcactctgtc ttccgtgctt ttcggccggc tcgtacaccg gatgtatgca
      421 tgctcagatg cgatagccgg caagaaatag agcgtagtca cttccacccg aacgactgcc
      481 gcgcttcccg cgcggggaca gttcatccac tccccgtcta gatccttcga ctgcgcacga
      541 gtatgtgctt cgctcaggat aaagtgattt gaactacccg cttgtcgcac tactagaccc
      601 aacgtagcat tgtgcaccga tgcgttcgtt cctcgcgggg ttttttctcc ttcttctgat
      661 gccggctttc gcgctcgccc aggctaaggg cgaagtggaa agcattggct tcaacaacag
      721 ctatcgcccc gacgcgtgga cgccgatggt cgtccgcctg cggccggaga cgaacgaacc
      781 cggcacttat cagatccagg tctggcaaca cgacatcgat ggagatcggc cggtcttcac
      841 gcggcagatc acgctcaatg gcgctgacca ggcgcgcgag cagcgcttct ggatgtactt
      901 cctgccgcag ccgatcgaca aggggttgcc cgatccaacc aacggcggaa cgctcaaaga
      961 cctgcagcgc gagctgccgg tgtttctctg caccgccagc gggaagcaga tcacgcaatt
     1021 gccgatcacc tcgacgctgc tgaacgtcga tccggtgcgc gactacgcgc agcagccgcg
     1081 cagcgccaag ctgctgctgg ccatcagtga cggcaccagc acccccgcgt ggcgcgacta
     1141 cgaatccgcg ctgggcttgg tcgaagacgt cgtcgtcgtg aacctgcagg cgaaagacct
     1201 gccggaagat ccgatcgggt atgaaggcgt cgatgcaatc gtctggttga acgctgaccc
     1261 ggctgatctg gaccgcggcg gggcgcacaa gctggcgacg ctgcaggatt acgtccgctc
     1321 cggcgggcat ctggtggtca cccagccgac gacggactgg caaaagatcc tcggcttcgc
     1381 cgacatgctg ccggtgacgg tgctgggcgt tgaagacaag aacgacctgg agccgctgcg
     1441 ctcgatggcc aagccgctcg atccaatcga ggcgctcaag ccgcgggtcg atcaatggct
     1501 tcgcccggtc ggtccgtttc agtttgccaa ggcgaaagcg aaatcccgcg cggtggttga
     1561 ggcgtggatc gactggcacg gcgacggttc gtacacggac gccacgccgt acatcgcgcg
     1621 ctccccgtat ggcttgggcg cggtgacgtg ggtggcgcag gacctgggca accccgccat
     1681 cgccggcaag accgccaacg gcgcgggctg gccttacgtg tgggacaagg tctttggttg
     1741 gaagaacgac acgtacgttc cgccaccggg tgtgtcgaag gacaacgaag acctcaagcc
     1801 gcggatggac ctgtatcgcc caggcagccc gatcgatctg ggttacccgc tcacgcaggg
     1861 gttgaacctg gacgcccgcg gggcgttcct gatcttcatc gccgtcgcgt ttttcttcgt
     1921 ctacctgatc gtcgcaggtc ccggcagcta tctctatctg gcttcgaaga agcgagcgcg
     1981 cttgagctgg ttcttcttcg gtggcgccgc aatcgccgcg acgctgctga ccgtcgccgt
     2041 cgtcaaagcg gtgatgcact atccaccgca gctgcggcac ctgacggtcg tccgcgtcgc
     2101 gccggggcaa cccacttaca tctacagccg attcggcctc tacatcacgc acgatggcga
     2161 tcaatccatc gagttgaccg gcacggcgca cgatggcgtc agttatctgt ccggctacgc
     2221 cgagcacccg cagcagctgg gtgacgtgac cgagttcccc gcccccgccg attacaccgt
     2281 gccggtgcgc gacatcgcgt ccatcgatcc gcccgcgctc accgtgccgt atcgaagcag
     2341 cttgaagaaa ttccaggcga agtggatcgg cgatctttcg cagcaggtcg gaaaattcac
     2401 cgcgtcgaat gtgaagatga cggtggaaga cggccgaacg ctgcttagcg gcgcgatcac
     2461 caaccagacc aacaacgact tcaacgatgt gtacatcgcc tatcacgggc agagcaaccg
     2521 cgatgaagat cgcctgatct acatcccgct gtggaaacgc ggcgtgacgc tcgacctgca
     2581 aaaggacctg gccaaggcgc cggtcgtcgg tcagctgggc aacaccaacc tggccgccat
     2641 tccgggtgac ggaaaaatca tcagcgatga gatcggcacg tccaacgtga agagcggcac
     2701 cgcgcgcgac atgcactgga tcggcttctg gtatcgcaag ttttacaaca gccagtatca
     2761 cgacgcgaac accgaagacc ccaggtacac ctatgtctat ccgatgctca gcctgttcga
     2821 ccgcctgccg ccggcgtgga acaaacccga cgtggccagc accggtgtgg gttcaacgtg
     2881 ggatcgcttc gagctatacc gccgcggcgg gcgcatgctg aacgtctcgc aaagcctgat
     2941 ggccggccag ctggccatcc tcgccagcgc acagggcccg ctgccgatgc cgatggaaat
     3001 caacggcgac aaagccgaag gccagggaac gaccttttac cagttcctgt taccgatcga
     3061 ccgcggcgac gtggataaac cgacgacgca accggcgggc tgaggg
//
LOCUS       DATIMH010000956         6915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2538238, whole genome shotgun sequence.
ACCESSION   DATIMH010000956 DATIMH010000000
VERSION     DATIMH010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538238"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VLI90_16565"
     CDS             <1..190
                     /locus_tag="VLI90_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV15878.1"
                     /translation="VRRLRAKGDRTPVIMISGQTETDVIEIARKAGVNEYLPKPLSTP
                     MLKAAMEKTVPGWASRAA"
     gene            247..318
                     /locus_tag="VLI90_16570"
     tRNA            247..318
                     /locus_tag="VLI90_16570"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:279..281,aa:Gly,seq:gcc)
     gene            complement(601..1407)
                     /gene="mutM"
                     /locus_tag="VLI90_16575"
     CDS             complement(601..1407)
                     /gene="mutM"
                     /locus_tag="VLI90_16575"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HSV15879.1"
                     /translation="MPELPEVQTVVDTLKPKVLGKRLTGVVLHRADILQPLGFDLTRQ
                     LTARTINGIHRRGKKIVFELNGGEWFYIHLGMTGRLTVETESTPTLPHTHLVLKFAKY
                     EVRFRDPRRFGGVFWLGRDNTADANMGPEPLQIRPAQLAKRLAKTTRAIKNALLDQSV
                     IAGLGNIYVDESLFRAGLHPLLPANQLSADDVSRLNRAIKTTLRRALRHRGSTLRDYL
                     DANGEAGAFQKLHAVYDREGQRCRRCKQSIQRIVLGGRSTHFCPECQPAR"
     gene            1536..2627
                     /locus_tag="VLI90_16580"
     CDS             1536..2627
                     /locus_tag="VLI90_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSV15880.1"
                     /translation="MESQLGIVQPTQTDLAAEELAAVEKLQKSYHDMKAELGRVIVGQ
                     DAVIEELLIALFCKGHALLVGVPGLAKTLLISTLAKTLGLSFSRIQFTPDLMPSDITG
                     TEVIEEDKTTGGRTLRFVRGPVFANVILADEINRTPPKTQAALLEAMQEHQVTAGGKQ
                     HRLPQPFFVLATQNPIEQEGTYPLPEAQLDRFMFNINVGYPTEDEEFQIVRMTTQGEK
                     IELKHVLSGEEVMALQDIVRKVPVADHVIRYALKFSRLTRRTEGGCPDFVNDFVGWGA
                     GPRASQYLILAGKARALLKGRYHVSTEDIRQVALPVLRHRIVTNFNAEAEGIKSDTIV
                     QKLIDFIPRQQNDELDRTAAKLMRDQPAA"
     gene            2984..3244
                     /locus_tag="VLI90_16585"
     CDS             2984..3244
                     /locus_tag="VLI90_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15881.1"
                     /translation="MTIENLKELKQKHPFEKFTIHMSDGKSFNVDDPEDLVIRRDWKV
                     DVLVLQPRGRFSFVYLKNVTHITGEGKLPRFKGRRTRGDDQE"
     gene            3257..4183
                     /locus_tag="VLI90_16590"
     CDS             3257..4183
                     /locus_tag="VLI90_16590"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSV15882.1"
                     /translation="MSETLTDYRKYLDPRTLARISSLDLRARLIVEGLTSGMHRSPYQ
                     GISVEFAQHRPYAFGDDIRHVDWKQYAKTDKIYLKQYLEETNLHLICVVDASESMGFG
                     TVKGDGDATWTKYDHATAIAASLSYMAIQQQDSAGLAIFDQALSRFFKPSNSPGQWKI
                     ITQELQQVPRWSKTNTGKILDQLAEKLTHRSLIVILSDFFDDLEGIKMGLRHLRYKKH
                     EMMVFQLLDPAEIEFPFEDVTMFKGLEEMGELLTEPRSLREGYLDQLRQFTDDLQKMC
                     RGMHIDFTRMNSGEPLYTTLSGFLATRSASMK"
     gene            4215..6509
                     /locus_tag="VLI90_16595"
     CDS             4215..6509
                     /locus_tag="VLI90_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HSV15883.1"
                     /translation="MSGRALLFIVHRSSFIVPMQSASNILAFSFLSPSFAIAGLVLAS
                     LPIIIHILNRRRFKTIDWAAMDFLLRAMRKNRKRLRFEQWLLLATRCLVLALLGFALA
                     RPLSCSENSMAGVGQRTGLHVIVLDNSYSMAYEADRPNAKTHFEHAKKLIEQMIDQLA
                     PGGESIAVITAARPATAVLAKPTYDLQQARAAVDRIAQTEGGTDLAGALRLAIDVGRE
                     NEKQANKNLYLLTDATRSAWQTPESEAVKFAGQELAELYHITNFNLSKGRQWNQAVLD
                     VRPGSNLVTAKSNFGSEFLADVKGFGDARDATIQWRLDGNVLPGGGTVHLAADTPPQT
                     QSQAVIKTGGPHVVTVSLLGDDRLKLDNTRSRVVNVASELKVLIVEGQRSTGPLGGSG
                     AFLQLALAPPRDGATSGNASQSDSYIAPELISDLELSNRVLTDYRAVLLCGVGQIQPA
                     QADQLQHFVEAGGTLILFMGEPVSSDNYNSVLLPRKLMPGPLTKRMNAGADQRGFVFD
                     FNPNGVLHPLLKAFANQEKTGLDTAQVYTYWQADVPNDPQIRVLNYRAADGASTRPGG
                     ASDPAITINTLGRGRVVFVSTTANADWTTLPAKPAYVALMQELLSGSINAGDAWMNLA
                     VGQRLDVPTNIKFTSTPTLVDPQSKVIPLEPTTTADGSPSFRSPPLNAYGVYTLATAN
                     GNVPVAVNVPAADEADVQTIDDAAIKSALGGIDAVMADDQLPRQAESVTAGNDLGWNV
                     MGVVMIFVALEAFLAMRFGHYRRK"
     gene            6678..>6915
                     /locus_tag="VLI90_16600"
     CDS             6678..>6915
                     /locus_tag="VLI90_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15884.1"
                     /translation="MHRLLEILLGLRRGFLSREGDLSVQFNPQWPWQAHVGAGVWNFV
                     LAALAAALVIYVYRRDGRSRAVRISLGILRGGLLL"
BASE COUNT         1370 a         2110 c         2170 g         1265 t
ORIGIN      
        1 ggtccgccgc ctgcgggcca agggcgatcg gacaccggtc atcatgatta gcgggcagac
       61 cgagacggac gtgatcgaaa tcgcgcgcaa agccggcgtc aacgaatacc tgcccaagcc
      121 gctgagcacg ccgatgctca aggccgcgat ggagaagacc gtccccggtt gggcgagtcg
      181 ggcggcctga gcgccgcttg aaagcggaac ccctcgccgc taccatactc cgacaataaa
      241 ttgctcgcgg gcgtagctca gtggtagagc gtcacgttgc caacgtgaat gtcgagggtt
      301 cgaccccctt cgcccgcttc aacaacgacc cggcgtcaca cgacgccggg ccgttttctt
      361 tggcgtcaaa gtcagccaaa agagtgagat cttatgtttg gccggcacct tgggaccgac
      421 gtcctcaact ttcatccctg tcatgtggga cggtggcgaa gccgtcggtc cggattttta
      481 actgaaccac tcgcggctac caagatactc tgacccgacg catccgggtc gtcgcgagta
      541 cgctccgaac ccggcgtggc gggatccgac agcatccgac cgggctgcac gaaatagctt
      601 tcagcgcgcc ggctggcatt cggggcagaa gtgtgttgag cggccgccca ggacgatgcg
      661 ctggatcgac tgcttgcacc gccggcagcg ctggccttcg cggtcgtaga ccgcgtgcag
      721 tttctgaaac gccccggctt cgccattggc gtccaggtag tcgcgcagcg tgctgccgcg
      781 gtgccgcagg gcgcggcgga gcgtcgtctt gatcgcgcgg ttcaggcgcg acacgtcatc
      841 ggcggacagc tggttcgccg gcagcagcgg gtgaagtccg gcgcggaaga gcgactcgtc
      901 cacatagata ttgccgagcc ccgcgatcac cgactggtcg agcagcgcgt ttttgatggc
      961 gcgcgtcgtc ttcgccaggc gctttgccag ttgagccggc cggatctgca gcggttccgg
     1021 tcccatgttg gcgtcggcgg tgttgtcgcg gccgagccag aagacgccac caaatcgccg
     1081 ggggtcgcgg aagcggacct cgtacttcgc aaatttcagc acgaggtgtg tgtgcggcaa
     1141 cgtcggcgtg gattcggtct caaccgtcaa tcggccagtc atgccgaggt ggatgtagaa
     1201 ccactcgccg ccgttgagtt cgaacacgat cttcttcccg cggcggtgaa tgccgttgat
     1261 tgtccgcgcc gtcagctgcc gggtcagatc gaacccgagc ggctgcaaga tgtccgcgcg
     1321 atggagcacc acgccggtta ggcgtttgcc cagcaccttc ggtttcagcg tatcgacaac
     1381 tgtttgaact tccggcagtt ccggcatgat tgtgcaactt tcgctctggc ggtccgtcct
     1441 atctcagagg cctattatag gttagactgc gtgcccgcgc gctcgtgcat gcttttgccc
     1501 aatcgatcaa caactacgga ccattaagga gccgcatgga atcacagctc ggcatcgtgc
     1561 aaccgacgca gaccgacttg gccgccgagg agttggcggc cgtcgaaaag ctgcagaaga
     1621 gctatcacga catgaaggcc gaactgggcc gggtgatcgt agggcaggac gcggtgatcg
     1681 aggaactgct gatcgcgctg ttctgcaaag gccacgcgct gctggtcggc gtgccggggc
     1741 ttgcgaagac gctgcttatc tcgacgctgg ccaagacgct ggggctgagc ttcagccgaa
     1801 tccagttcac gccggacctg atgccgagcg acatcaccgg caccgaggtg atcgaggagg
     1861 acaagacgac cggcgggcga acgctgcgct tcgtgcgcgg gccggtcttc gcgaacgtga
     1921 tccttgccga tgagatcaac cgcacaccgc ccaagacgca ggcggcgttg cttgaagcga
     1981 tgcaggaaca tcaggtgacg gccggtggga agcagcaccg cctgccgcag ccgtttttcg
     2041 tcttggcgac gcagaacccg atcgagcagg aaggaaccta tccgctgccc gaagcgcagc
     2101 tggatcggtt catgttcaac atcaatgtgg gctatccgac cgaggacgaa gaatttcaga
     2161 tcgtccgcat gacgacgcag ggtgagaaga tcgaactgaa acacgtgctg agcggcgaag
     2221 aagtgatggc gctgcaggac atcgtccgca aagtgccggt ggcggatcac gtcattcgct
     2281 acgcgctgaa gttcagccgg ctgacgcgcc gcaccgaagg cggttgcccc gacttcgtca
     2341 acgacttcgt cggctggggc gccggaccgc gggccagcca atacctgatc ctcgccggca
     2401 aggcccgcgc gttgctgaag gggcgctatc acgtcagcac ggaagacatt cgccaagtcg
     2461 cgctgccggt gctgcggcat cgcatcgtga ccaacttcaa cgccgaggcc gaggggatca
     2521 agagcgacac gatcgtgcag aagctgatcg acttcatccc gcggcagcag aatgacgaat
     2581 tggatcgaac cgcggcgaag ctgatgcgcg accagccggc ggcgtgacag gagagcttca
     2641 tggcccttcg ccgattttgg ataactttcg aagatggggc caaggacctg cccctcggtt
     2701 tcggtgtcac ggcattcgat gagaaggacg caatggagat tgttgaggga tggctgactg
     2761 aaaataagtt tgtggtgccg acccgtcagt gcatcttaca tgacgtggat gtgagctcat
     2821 tgcaggggtg gcggatttgc cgctctctca cttcggtatc ccttccaacc gcggcctgtg
     2881 gtatccgcga tgcaacatcg gatccgaata tccgctgcac tgagcgatcc gctgccgact
     2941 cgaaggtcaa ttcgagatct ggaacacatg ctggaaccgt tctatgacga tcgagaacct
     3001 gaaagagctt aagcagaagc acccattcga aaagttcacc atccacatgt cggatggcaa
     3061 gtcgtttaat gtggatgatc cggaagatct ggtgattcgc agggattgga aggttgatgt
     3121 gctggtgctg cagccacgcg ggcgattctc gttcgtctac cttaagaacg tgacgcacat
     3181 cacgggcgag gggaaacttc ccaggttcaa aggccgccgc acgcgcgggg acgatcaaga
     3241 gtagagcaga gttgtaatgt ccgaaaccct caccgactat cgcaagtatc tcgaccctcg
     3301 gacgctggcg cggatcagca gcttagatct gcgcgcgcgg ctcatcgtcg agggcctcac
     3361 cagcggcatg caccgttcgc cgtatcaggg catcagcgtt gaattcgcgc agcatcggcc
     3421 gtacgcgttt ggcgatgaca ttcggcacgt cgattggaag cagtacgcga agaccgacaa
     3481 gatttatctc aagcagtatc tggaagagac gaacctgcat ttgatctgcg tcgtcgacgc
     3541 cagcgagtcg atggggtttg gaaccgtcaa gggcgacggc gatgcgacgt ggacgaaata
     3601 cgatcacgcg acggcgatcg ccgcgagctt gtcttacatg gcgatccagc agcaggactc
     3661 ggccggcctt gcgatcttcg accaggcgct cagtcgcttt ttcaagccga gcaattcgcc
     3721 ggggcagtgg aagatcatca cgcaggagct tcagcaggtg ccgcgctgga gcaagaccaa
     3781 taccgggaag atcctggacc agctggccga gaagctcacg caccgcagcc tgatcgtgat
     3841 cctgtcggat ttcttcgacg atctggaagg cataaaaatg ggtctgcgcc acctgcgtta
     3901 caagaagcac gagatgatgg tcttccagtt gctcgacccg gccgagatcg agttcccgtt
     3961 cgaagacgtg acgatgttca agggcctgga agagatgggc gagctgctga ccgagccgcg
     4021 cagtctgcgc gaaggttacc tcgatcagct gcggcagttc acggacgact tacagaagat
     4081 gtgccgcggg atgcacatcg atttcacgcg gatgaacagc ggcgaaccgc tgtatacgac
     4141 cttgagcggt ttcctggcga cgcgatcggc gtcgatgaag tgagaagcga acgatgaacg
     4201 acgaacgatg aacgatgagc ggccgcgctc tgttgttcat cgttcatcgc tcatcgttca
     4261 tcgttccgat gcagagtgcc tcgaacatcc tcgccttctc cttcctctca ccgtcgttcg
     4321 ccatcgcggg gctggtgctc gcgtcgctgc cgatcatcat ccacatcctg aaccgccggc
     4381 ggtttaagac gatcgactgg gcggcgatgg acttcctcct tcgcgcgatg cggaagaacc
     4441 gcaagcggct ccgcttcgaa cagtggctgc tgctggcgac gcgatgcctg gtgctcgcgc
     4501 tgctcggctt cgcgctggcc cggccgctca gttgctccga aaactccatg gcaggtgtcg
     4561 gccagcgcac cggcttgcac gtcatcgtgc tcgacaacag ttattcgatg gcgtacgagg
     4621 ccgatcgacc gaacgccaag acgcacttcg agcacgcgaa gaaactgatc gagcagatga
     4681 tcgaccagct cgcccccggc ggcgaatcga tcgccgtcat cacggccgcg cgaccggcga
     4741 cggcggtgct ggccaagccg acgtacgatc ttcaacaagc gcgagcggcc gtcgatcgca
     4801 tcgctcaaac cgaaggcggc accgatctcg ccggcgcgtt gcgcctggcg atcgacgttg
     4861 gccgcgaaaa cgaaaagcag gcgaacaaga acctctatct gctgaccgat gcaacgcgca
     4921 gcgcctggca aacgccggag tcggaggcgg taaagttcgc cgggcaggaa ttggccgagc
     4981 tgtatcacat cacgaacttc aacctgtcca agggccgcca gtggaaccag gcggtgctgg
     5041 acgttcgccc cggctcgaac ctcgtcaccg ccaagagcaa ctttggttcc gaattcctcg
     5101 ccgacgtcaa aggctttggg gacgcgcgcg acgcgaccat tcaatggcga ctcgatggca
     5161 acgtcttgcc cggcggtggc acggtgcacc ttgccgccga cacaccgccg cagacgcagt
     5221 cgcaggcggt catcaaaacc ggcggcccgc acgtcgtcac cgtctcattg ctcggcgacg
     5281 accggctgaa gctcgataac acgcgctcgc gcgtcgttaa cgtcgcgtcg gaattgaagg
     5341 tgctgatcgt cgagggccag cgcagtacag gcccgctcgg tggatcgggc gcgttcctgc
     5401 aactggcgct ggcgccgccg cgcgacggcg cgacgagcgg caacgcgtcg caaagcgaca
     5461 gctacatcgc gccggaattg atcagcgacc tggaactcag caaccgcgtg ctcaccgact
     5521 atcgcgccgt cctgctctgc ggcgtcggcc agattcaacc ggcgcaggcg gatcagcttc
     5581 agcacttcgt cgaagccggc ggcacgttaa ttttgttcat gggtgaaccg gtcagcagcg
     5641 acaattacaa cagcgtgctg ctgccgcgga agctgatgcc gggcccgctg accaagcgga
     5701 tgaacgccgg cgccgatcag cgcggtttcg tcttcgactt caatcccaac ggtgtgctcc
     5761 acccgctgct caaggctttc gccaaccagg aaaagaccgg cctcgatacc gcgcaggtct
     5821 acacctattg gcaagcggat gtgcccaacg acccgcagat ccgcgtgctg aactatcggg
     5881 ccgccgatgg cgcgtccacc cggcctggtg gggcgagtga tcccgccatc accatcaaca
     5941 cgctcggccg cgggcgcgtc gtcttcgtct cgaccaccgc caacgccgac tggaccacgc
     6001 tgcccgccaa gcccgcgtac gtagcgctga tgcaggagct gctcagcggc agcatcaacg
     6061 ccggcgatgc gtggatgaac ctggccgtcg gccagcgcct ggacgtgccg accaacatca
     6121 agttcaccag cacacccacg ctggtcgatc cgcagtctaa ggtgattcca ttagaaccaa
     6181 ccacaacggc cgatggttcg ccgtcgttcc gcagcccgcc gctgaacgca tacggcgtct
     6241 acacgctggc caccgccaac ggcaacgtac ccgtagccgt gaacgtgccc gccgccgacg
     6301 aggcggacgt gcagacgatc gacgatgcgg ccatcaaatc cgccctcggc ggcattgatg
     6361 cggtgatggc ggatgatcaa ttgccgcggc aggccgaatc ggtaacggcg gggaatgatt
     6421 tggggtggaa cgtgatgggg gtggtgatga tttttgttgc gttggaagcg tttttagcga
     6481 tgaggtttgg gcactaccgt agaaagtagg aaagtccgaa tgacgaaatc cgaatccccg
     6541 aatgaaatcc gaatgaggaa tgacggaccg gcgccacgcc cttcgtcatt cggatttcgt
     6601 cattcattcg gggattcggg tttcggactt cgtcattccc acagttgtcg taggatcggc
     6661 cgaagcgatt agtcaccatg catcgtctcc tcgaaatcct cctcggcctt cgccgcggct
     6721 tcctgagccg cgagggggac ctgtcggtcc agttcaaccc gcagtggccg tggcaggcgc
     6781 acgtcggcgc gggcgtttgg aacttcgtcc tcgccgcgtt ggcggcggcg ttggtgatct
     6841 acgtctaccg ccgcgatggc cggtcgcgcg cagttcgcat ctcccttggc atcctgcgcg
     6901 gcgggctgct gctgc
//
LOCUS       DATIMH010000957         6497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3695183, whole genome shotgun sequence.
ACCESSION   DATIMH010000957 DATIMH010000000
VERSION     DATIMH010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3695183"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VLI90_16605"
     CDS             complement(<1..764)
                     /locus_tag="VLI90_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15885.1"
                     /translation="MSAAAGAALLATAPLARAQVTPSQTNSDQLNQLIVSDVFETTPN
                     KNAAGEGYINGEFRYLDFGHDIKQYLYELKGDYGITDQITVGGWLPIYDAKVGDSHTG
                     IGDITFFGQYKFDQLINPDVVNLTGQVDVVLPTGSRNRLRDTGKFGVRPIVEAYKNFG
                     QVGPGTIGAYGLLGVTITTNPDVRIGLAGTYEWQKIVGIIEFDDLAGDKRGAPLVTLT
                     PGVAYRGLNPFEFELGFPFGLNDASPDWGIVLKATFA"
     gene            complement(946..2805)
                     /locus_tag="VLI90_16610"
     CDS             complement(946..2805)
                     /locus_tag="VLI90_16610"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HSV15886.1"
                     /translation="MNRPTTAGLRTSFVIESLEPRAYFAGVLFAPHAFYGAGPHPNAI
                     AAGDLNGDGKPDLVVANYTDGSVTVQLNRGDGTFAPAPTLIVGNGPESLAIADFNGDG
                     HADIVVANSGTVPSDKNSSPGDTLSILLGNGDGTFAPKVDIAAPGAPQSVITADMNGD
                     GHVDLITANRFESTISVWLGNGDGTFTPDAAGHVAVGFHPSAVAAGDLNLDGKLDLAV
                     ANNGSNTISVLLGNGDGSFGSMKFFVANNAPRAIAIADITADGKPDVITANLSQSVTV
                     LPGLGTGLFGPTVNSFGGNVPFSIALADLNNDDRLDVVTAAQNGNTVGVLVRSRIFNG
                     FQGLSNFPAGAGPTAVCIADFNGDGRPDIATANFLFNNIGVLINIMQPTQTTLVTSEN
                     PAPARAAVQFTAIVHQAGATPITGTPRGLVQFFDGTTLIGGVQLRGDGTAVFVRSNLA
                     VGAHGISALYIGDNRFASSIALGAVTQVITPAITGQALLQPIVHAVKFPPTLSAGSKG
                     NAQIIVQNQGDVATRGVLAVRLLLSADTTVSEDDVPLTGSVGDLSLHFAPHAAISFPD
                     TFTVPDGVAPGMYFLLAQLVGVSGFAAGEVSDLAASSATVANATAPTTPAGAL"
     gene            complement(3017..4771)
                     /locus_tag="VLI90_16615"
     CDS             complement(3017..4771)
                     /locus_tag="VLI90_16615"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSV15887.1"
                     /translation="MLGQEAPATRPAFTVLLSPGVAPCTVHVNGLTIAPQAGTPLTAH
                     YDWDFGDPRGRFDKLSGFNAAHTYNFPGQYTIALLLTDEAGHQITASATVTITADTRH
                     RVFVAPEGSDMNTGTSPDTPYQSLARAFKAMPNDGELLLRAGATYNVTSSLHLNRRDV
                     LIGRFGDGPDPVVMRIKGNGTSTFSLDKNCDGVTVEHITFDSPNAVGDNQEAPKVGAI
                     GVLCSGSSRNITVRNCTFRNVDDGVNANGKPTGLLVQDCSAPLVTGVRGYLIWGEGTD
                     HVYLGNSAANSTREHNVRLSGVQRVLIDSNHFANLDRHDVDKYDGSKGCIEMHRGEFA
                     WISHNDVTGGTIRVGPRGGQFEGPESVTEWCVIDANALTNTGILAYGGSRHIMVRNNI
                     IRWDGNTAIVINGAPTSEDITIVHNTAINNGELGAFFRLWGHVNGITLSNNLFVAPNL
                     KPGSHGSSAVGIDETDLASFSEISHNVWPDPGSEKGMCIIGTGKSGGGLVSAQQWSSF
                     SVVKQDRFANVTVDDRGVPQAGSAAEGYAVPTRAVTHDYTGRPRPLGERTVGALEPGL
                     PPATHPAPVPSPGTPGEG"
     gene            complement(5054..5944)
                     /locus_tag="VLI90_16620"
     CDS             complement(5054..5944)
                     /locus_tag="VLI90_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15888.1"
                     /translation="MKSTLTLALQAALVTFVFAAICSAEDQTSTRHFGGLLDRRSTYG
                     DFWFPEPLRGPEMDVDSEVRVDYFHSESSNTQSNEVVAEIEHNIGLLTFEVEIPYVRE
                     SESFFDPATRQISHDVSEGLGNIELAARHPVFQLVSPDERVDYTLVAALEVAVPTKSE
                     ISHDTEIVPQLFQLLRVGRHFSVQASAGYSALIGPVEGGTNTLEYNVVLGWNIEREEF
                     RLPGIDRIIPIFELNGERGFTGEDQGINRLFGTAGMRFNFRPIGTVQPRIGLGYMFPI
                     DDGGRAEMDWGVVTSLVFEL"
     gene            6086..>6497
                     /locus_tag="VLI90_16625"
     CDS             6086..>6497
                     /locus_tag="VLI90_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15889.1"
                     /translation="MRKQDIVLSGGFGTSTNSARAGFLPISLERAPVDAFEKIPVYLR
                     AAAPSPPPGAAGTDESKDSFRLYCAEHVRFTPAHRQRLFDHGVKFVYIPMTHQSRFRQ
                     QTEDRLKELATDATVAVSVKSEIIYETSVELVNEL"
BASE COUNT         1170 a         2018 c         2096 g         1213 t
ORIGIN      
        1 gcgaacgtcg ccttcagcac gatgccccag tcgggcgacg cgtcgttcag gccgaagggg
       61 aagccgagtt caaattcaaa cgggttcagc ccgcgatacg cgacgcccgg cgtcagcgtg
      121 accagcggtg cgccgcgctt gtcgccggcc aagtcgtcga attcgataat gccgacgatc
      181 ttctgccatt catacgtgcc ggccaggccg atgcgcacgt cggggttggt cgtgatcgtc
      241 acgccgagca gaccgtacgc gccgatggtg ccgggaccga cttggccgaa attcttgtac
      301 gcttcgacga tcggccgcac gccgaacttg cccgtatcgc gcagccgatt tcggctaccg
      361 gttggcagca ccacgtccac ttgccccgtc aggttcacca cgtccgggtt gatcagctga
      421 tcgaacttgt actgcccgaa gaacgtgatg tcgccgatgc ccgtgtggct gtcgccgacc
      481 tttgcatcgt aaatcggcaa ccacccgccg accgtgatct ggtcggtgat gccgtagtcg
      541 cccttgagct cgtacaggta ctgcttgatg tcgtgcccga agtcgaggta gcggaattcg
      601 ccgttgatat acccttcgcc cgccgcgttt ttgttcggcg tggtctcaaa gacgtcggac
      661 acgatcagct gattgagctg atccgaattc gtctgcgacg gcgtcacctg cgcccgcgca
      721 agcggagcgg tggccaacag cgccgcgccc gcagccgcgg acaacagcag cgcgccaaat
      781 gtggaccgag ccttgcgcgc aagactcccg agagaaacga tccgattcat catggtcacc
      841 ttcggttgaa aaacattccc tgtggcgggg aatatgcagt gcggcaagcg cacgtgtcaa
      901 ctggagtttc agttatagac agcgggggat gccagcggct ttgtgctaca gagccccggc
      961 gggagtggtc ggggcggtgg catttgcaac cgttgcgctc gacgccgcta gatcgctcac
     1021 ctctcccgcg gcaaaaccgg agacgccgac cagctgcgcg agcaggaaat acatgcccgg
     1081 cgcaacgccg tcgggcacgg tgaaggtatc gggaaacgag atggccgcgt gcggggcaaa
     1141 gtgcagcgac aggtcgccga cgctgccggt caacggcaca tcgtcctcgc tgacggtcgt
     1201 gtcggccgac agcagcagcc gcaccgccag cacgccgcgg gtcgccacgt cgccctggtt
     1261 ctgcacgatg atttgcgcgt tgcccttgct gccggcgctg agcgtcggcg gaaatttcac
     1321 cgcgtgaacg atgggttgca gcaacgcctg cccggtgatg gccggcgtga tcacctgcgt
     1381 cacagcgccc agcgcgatgc tggacgcgaa gcgattgtcg ccgatgtaca gcgcgctgat
     1441 gccgtgcgcg ccgaccgcca ggttggaccg cacgaacaca gcggtgccgt cgccccgcag
     1501 ttgcacgccg ccgatcagcg tggtgccgtc gaagaactgc acgagccccc gcggtgtgcc
     1561 ggtgatcggc gtcgcgcccg cctggtgcac gatcgccgtg aactgcaccg ccgcccgcgc
     1621 cggcgccggg ttttcactgg tcaccagcgt cgtctgcgtc ggttgcatga tgtttatcag
     1681 cacgccgatg ttgttgaaca gaaagttcgc cgtcgcgatg tccggccggc catcgccgtt
     1741 gaagtccgcg atacatactg ccgtcggacc cgcgccggct ggaaagttgc tcagtccctg
     1801 gaagccgttg aagatccgcg atcgcaccag cacgccaacg gtgttgccgt tttgggcggc
     1861 ggtgaccacg tcaagccggt catcgttgtt caaatccgcc agtgcgatcg agaacggcac
     1921 gtttccgccg aacgaattca ccgtcggccc gaacaatccc gtccccagcc ccggcagcac
     1981 cgtcacgctt tgcgagaggt tcgcggtgat cacatccggc ttgccatcgg cggtgatgtc
     2041 ggcaatcgcg atcgcgcgcg gcgcattgtt cgcgacgaag aacttcattg acccgaacga
     2101 tccgtcgccg ttgcccagca ggacgctgat cgtatttgac ccgttgttcg ccaccgccag
     2161 gtcgagcttt ccgtccaggt tcaaatcgcc cgccgcgacg gccgagggat gaaacccgac
     2221 agccacatgc cccgcggcgt cgggtgtgaa cgtgccgtcg ccgttgccga gccagacgga
     2281 gatcgtgctc tcgaatcgat tggcggtgat cagatcgacg tgcccgtcgc cgttcatgtc
     2341 ggcggtgatg accgactgcg gcgcgcccgg cgcggcaatg tccaccttcg gcgcgaacgt
     2401 cccatcgccg ttgccgagta ggatgctgag cgtgtcgccc ggcgaactgt tcttatccga
     2461 cggcacggtg ccggagttgg cgacgacgat atccgcgtga ccgtcgccgt tgaaatccgc
     2521 aatcgccagt gactccggcc cgttgccgac gatcagcgtc ggcgccggcg cgaacgtgcc
     2581 gtcgccgcga ttcaattgca ccgtcacgct gccatcggtg tagttcgcca cgaccaggtc
     2641 aggcttgcca tcgccattta aatcccccgc ggcaatcgca ttcggatgcg gccccgcccc
     2701 gtaaaacgcg tgcggcgcaa acaacacccc cgcgaaatac gcccgtggct cgagcgattc
     2761 aatgacaaat gacgttcgca gcccagcggt tgttggacga ttcaatcgag tgactcctgg
     2821 cgcgctagac gcggcagcgt agccttgccc gcgcgttcgt gcaaaggcgc gagtgggttg
     2881 cgagcattgg gggatacgac tttgatttgc cggctcgatt ctggtccgtc cccctcgccc
     2941 ggtacgccgg gagagggtcg gggtgagggt gatttcgaac ttcgagcggc cctcgataaa
     3001 cgatagcacc ctcaccctaa ccctctcccg gtgtaccggg agaggggacc ggagccgggt
     3061 gcgttgccgg tggcaggccc ggttccagcg cgcccaccgt tctttcacct agcggccgcg
     3121 gtcggccagt gtaatcatgg gtgacggcgc gggttggcac ggcgtagccc tcggctgcgc
     3181 tgccggcctg cggcacaccg cgatcatcca ccgtcacatt ggcaaaccgg tcctgcttta
     3241 caacgctaaa actgctccat tgttgcgcgc tgaccagccc gccaccggat ttgccggtgc
     3301 cgatgatgca catccccttc tcagaccctg gatcgggcca aacgttgtgc gaaatctcgc
     3361 tgaagctggc caagtccgtt tcatcgatac ccaccgcaga tgagccgtgc gagcccggct
     3421 tcaaattcgg cgcgacaaac aggttattac tgagcgtgat gccgttcaca tggccccata
     3481 acctgaaaaa cgccccaagc tcgccgttgt tgatggccgt gttgtgaacg atcgtgatgt
     3541 cttcggaggt cggcgcgccg ttgatgacga tggcggtgtt gccgtcccag cggatgatat
     3601 tgttgcggac catgatatgc cgcgacccgc catacgcgag gatgccggtg ttggtgagtg
     3661 cgttggcgtc gatgacgcac cactcggtga cggattcagg cccttcgaat tgcccaccgc
     3721 ggggaccgac gcggatggtg ccaccggtca cgtcgttgtg gctgatccag gcaaactcgc
     3781 cacggtgcat ttcgatacag cccttggagc cgtcgtactt gtccacgtcg tggcggtcga
     3841 ggttggcaaa gtggttggaa tcgataagaa cgcgctgcac gccggacagg cggacgttgt
     3901 gctcgcgcgt ggagttggcg gcggagttgc caaggtagac gtgatcggtg ccttcacccc
     3961 agatcaggta tccgcgcacg cccgtgacca gcggagcgct gcagtcctgc accagcagcc
     4021 cggtcggttt gccgttggca ttcacaccat cgtcgacgtt gcggaacgtg cagttgcgca
     4081 cggtgatgtt ccggctgctg ccggagcaga gcacgccgat cgcgccaacc ttgggcgctt
     4141 cctgattgtc gccgacggcg ttggggctat cgaaggtgat gtgctcgacg gtgacgccat
     4201 cgcagttctt gtccagcgag aacgtgctgg tgccgttgcc tttgatgcgc atgacgaccg
     4261 ggtccggccc atcgccgaac cggccgatca gcacatcccg gcggttgagg tgaagacttg
     4321 aggtgacgtt gtacgtggcg cccgcgcgca gcagcagctc gccgtcgttg ggcatcgcct
     4381 tgaacgcccg agcgagggac tgatatggcg tatccggcga cgtgcccgtg ttcatgtccg
     4441 aaccttcggg ggcaacaaag acgcgatgcc gggtgtcggc ggtgatggtg acggtggcgc
     4501 tggcggtgat ctgatgtccc gcttcatccg tgaggagcag cgcgatcgtg tattgcccgg
     4561 ggaaattgta cgtgtgagca gcgttgaagc cggagagttt gtcgaatcgc ccgcgggggt
     4621 caccgaagtc ccagtcgtag tgggcggtga gtggcgtgcc ggcttgtggc gcgatggtga
     4681 ggccgttcac gtggacggtg cacggcgcga cgcccggcga aagcagcacc gtaaatgccg
     4741 ggcgagtcgc gggggcttct tgtccgagca ccgcgactgc gaagaggttc gcgagaagaa
     4801 gaactatgaa ggtgcgcgac acggtcattc cgccaatgat agccgcgccg cgagtcaccc
     4861 gcgtagcggt accgtagcac gggcgtctca cccgtgtttc gtgaacgcca cgaatgccgg
     4921 cggtactcca tcggcaaagc aatcgcgtca gcgaaagagt accgagcact gtcgctaacg
     4981 cgattgctgt gtcgatggcg taccatcgcc gtgcatgggt acacgcacca cgggcgagac
     5041 gcccgtgcta cggttacagc tcgaagacaa ggctggtgac gaccccccag tccatctcag
     5101 cgcggccgcc atcgtcaatc gggaacatgt atccgagccc gatgcgcggc tgcactgtgc
     5161 cgatcgggcg gaagttgaag cgcatccccg cggtgccgaa gaggcggttg atcccttggt
     5221 cttcgccggt gaacccgcgt tcgccgttca gttcgaagat ggggatgatg cggtcgatcc
     5281 cgggcaggcg gaattcttcg cgctcgatgt tccagccgag gacgacgttg tactccagcg
     5341 tgttcgtgcc gccttcgaca ggaccgatca acgccgagta accggcgctg gcctgtacgc
     5401 tgaagtgccg gccgacgcgc agcagttgga acaactgggg aacgatctcc gtgtcgtggc
     5461 tgatttcgga tttggtcggc accgccacct ccagggcggc gaccagcgtg taatccaccc
     5521 gctcgtccgg cgagaccagc tgaaacaccg gatgccgggc ggccagttcg atgttgccca
     5581 ggccttcgct cacgtcgtgg ctgatctgcc gtgtggccgg atcgaagaag gactctgact
     5641 cccgcacgta agggatttcg acttcgaagg tcagcagccc gatattgtgc tcgatctccg
     5701 cgaccacttc gttcgattgg gtgttggaag actcgctgtg gaaatagtcc acgcggacct
     5761 ctgaatccac gtccatctcc ggtccgcgaa gcggctcggg gaaccagaag tcgccataag
     5821 tgctgcggcg atcaagaagt ccgccaaaat ggcgggtgct cgtctggtcc tccgcggagc
     5881 agatcgccgc gaagacaaag gtgacgaggg ccgcctgaag ggcgagggtc agggtagatt
     5941 tcattggaac aaagtagttg agacgcagtc tcaacgcaag tgatcggtca taaagcgaaa
     6001 tgcatataga cccgacgatg agttggggcg cgccggtggc gattcacctc gcgttgaccg
     6061 aaaaaggact ttcgtcaggg tggatatgcg caagcaagac attgttctct cagggggatt
     6121 cgggacctca acgaattcgg cgcgggcggg attcctgccg atcagcctcg agcgcgcgcc
     6181 cgtcgatgcg ttcgagaaga tcccggtgta cctgcgcgcc gccgcccctt caccgccgcc
     6241 gggggcggcc ggtactgatg aatccaagga ttcgtttcgc ctgtactgcg cggaacacgt
     6301 ccgcttcaca cccgctcatc gccagcggtt gttcgaccac ggcgtgaagt tcgtttacat
     6361 cccgatgacg caccagagcc ggttccgcca gcagacggaa gaccggctga aggaactggc
     6421 caccgacgcg acggtggccg tctcggtgaa gtcggaaatc atttacgaaa ccagcgtcga
     6481 gctggtgaac gagttgc
//
LOCUS       DATIMH010000958         4948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3404605, whole genome shotgun sequence.
ACCESSION   DATIMH010000958 DATIMH010000000
VERSION     DATIMH010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3404605"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VLI90_16630"
     CDS             complement(<1..1128)
                     /locus_tag="VLI90_16630"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSV15890.1"
                     /translation="MSESQIIERSPTATIIRTGTNGQRLTIPNTLPILPIRNIVVFPG
                     TVMPLNVGRQKSKNLLDEVMPGEKIIGVVTQRNAETEDPQLDDLYSVGVACMILKLFK
                     LPDGNQSIIVHGLTRFRVKNLIQTDPFALGEIEVLEDTLEAGPGLDALIASVRQQANR
                     VIELSPNTPDEAAQVLASITQPSALADFLAANLQADTTEKQRMLEELDVEKRLRLIAA
                     RLATQLDVLELQSKIQSQVKENIDKSQRRYYLQEQLKAIRKELGDAEGVPGGGSEIDQ
                     LRAKLEAAKLPENVMKEATRELNRLEAIPSASPEYGVIRTWLQIVSELPWAITTSDKL
                     DLTEARKILDRDHFDLDKVKRRIIEYLAVLKLKRDRNVKASQ"
     gene            complement(1224..1652)
                     /locus_tag="VLI90_16635"
     CDS             complement(1224..1652)
                     /locus_tag="VLI90_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSV15891.1"
                     /translation="MIETAPDHPFQHISRNVNKIVEQMQKGYYNFRPNDCWAPNVNLY
                     ETDDNYLVCADLSGVEKEKIDVELSGNRLTIRGARGVPSSTDAEAAGASAERGKLRVH
                     VMEIDHGAFFREVELPEHVQRERITAHYLNGMLWIEIPKK"
     gene            1863..3242
                     /gene="rmuC"
                     /locus_tag="VLI90_16640"
     CDS             1863..3242
                     /gene="rmuC"
                     /locus_tag="VLI90_16640"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HSV15892.1"
                     /translation="MEYVFGIAALVIGSVIGYLLGERRTRGGVGELRSAVASAEQRCA
                     GLASQFDRQSAEIADLRGQLSTAEKECASMSAQLEGARQNLLEQRKLLDDANHQLKQA
                     FASVSAEALAKNNEAFLQLAKERFATLSTEAAGTLEQRKAQIEGLLKPMQELLNQYQS
                     RLGDIEKSRVESYSMLREQLGILTETQRTLNTQTHQLVSALRRPQTRGQWGEVTLRRL
                     VELAGMASRCDFTEQTTVDTEDGRQRPDMVVSLPGDRTIVIDCKASLDAFLDAAAAPD
                     EDVRRVHLQKHCQQVKLRARELAAKSYWSQFKTSPEYVVMFLPGEAFLYAAVEQDGAI
                     IEDTLKSRVIIATPTTLMALLRAIEFGWRQEAVTQNAEEIQKHGKDLYDRIVVLAQHF
                     TKLGGSLDAAVHSYNSAIGSLETRVLVTARKVAELGARSDRELPEPQLIDTRPRELGA
                     ALQPAADPS"
     gene            complement(4121..4558)
                     /locus_tag="VLI90_16645"
     CDS             complement(4121..4558)
                     /locus_tag="VLI90_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15893.1"
                     /translation="MADPFVVGSVIAFVGPDRLNRRAPRGGRQIDPHALPLLRFCSLQ
                     RLQAVLRNPRQPAFGPSRFDVSHSPRSPPEVGTRCEAMTVVLAVLLPCVLRAPGPRRA
                     MNFTIHTGPVRVIAPRSLFRAAFRYPIFTRPFHDWAECVRATL"
     gene            complement(4699..>4948)
                     /locus_tag="VLI90_16650"
     CDS             complement(4699..>4948)
                     /locus_tag="VLI90_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15894.1"
                     /translation="NRLAEASQAGQACARSNAGAKTSGRLLAGRLQEKRERRSSSHFQ
                     LLFLFRGLRSTAIGVSRITASHAGALLRFRIITLAMAC"
BASE COUNT          978 a         1430 c         1625 g          915 t
ORIGIN      
        1 ctgcgacgcc ttcacgttgc gatcgcgctt cagcttcagc accgcgagat attcgatgat
       61 ccggcgtttg accttgtcca gatcgaagtg atcgcggtcg aggatcttcc gcgcctcggt
      121 caggtccagc ttgtcggatg tggtgatggc ccacggcagc tcgctgacga tctgcagcca
      181 ggtgcggatg acgccgtact cggggctggc gctgggaatc gcctccaggc gattcagctc
      241 gcgcgtcgct tccttcatca cgttctcggg cagcttggcc gcctcgagtt tcgcgcgcag
      301 ctggtcgatc tcgctgcccc cgccgggcac gccctcggcg tcgccgagct ccttgcggat
      361 ggctttcagc tgttcctgca ggtagtaccg ccgctggctc ttgtcgatgt tttccttcac
      421 ctgcgattgg atcttgcttt gcaattcaag cacatccaac tgagtcgcca ggcgcgcggc
      481 gatgagccga aggcgttttt ccacgtccag ctcttcgagc atgcgctgtt tttcggtcgt
      541 atccgcctga aggttggcgg ccaggaaatc tgccagcgca gacggctgcg tgatgctcgc
      601 cagcacctgc gccgcttcgt caggggtgtt gggcgaaagc tcgatcacgc gattggcctg
      661 ctggcgaacc gaagcgatca gcgcatcgag ccccggcccg gcttcgagcg tgtcttccaa
      721 cacttcgatt tcgccaagcg caaacgggtc ggtctggatc aggttcttca cgcgaaagcg
      781 cgtcagcccg tgcacgatga tggactgatt gccatcgggc agcttgaaga gcttgaggat
      841 catgcaggcg acgccgacgc tgtacaggtc gtccagctgc gggtcttcgg tctcggcgtt
      901 gcgctgcgtg acgacgccga tgatcttctc acccggcatc acttcatcca ggaggttttt
      961 gctcttctgc cggccgacgt tcagcggcat cacggtgccg ggaaagacga cgatgttgcg
     1021 gatgggcaag atcggcagcg tgttggggat cgtcaaccgc tgcccgttgg tcccggtgcg
     1081 gatgatggtt gccgtcggcg agcgttcaat gatttgcgat tcggacattg gtgcgtagtt
     1141 gctagctgct gattgcccgt tgctagtgaa gaaggctccg tggattcact agcaactagc
     1201 aaccagcaac tagcgacttc ctctcacttt ttaggaatct caatccacag catcccgttc
     1261 aagtagtggg ctgtgatacg ttcgcgttgc acgtgttcgg ggagttcgac ttcgcggaag
     1321 aaggcgccgt ggtcgatctc catcacgtgc acgcggagct tgccgcgttc ggcgctggcg
     1381 ccggcggcct cggcgtcggt ggagctgggg acgccgcggg cgccgcggat cgtcagacga
     1441 ttgcccgaca gctccacgtc aatcttttcc ttctcgacgc cggacagatc cgcgcagacg
     1501 agatagttgt cgtcggtctc gtacaggttg acgttggggg cccagcagtc gttggggcgg
     1561 aagttgtaat aacccttctg catctgctcg acgatcttgt tgacgttccg gctgatgtgc
     1621 tggaaggggt ggtcaggtgc ggtttctatc atcgcgattg ctccacgcag atggcctctg
     1681 ctcgaacgtg cggcacatca tagacgtccc ctgcgcgatg ggataggcgc atttgcgaca
     1741 gtcggacggc aactttttcg gaggcgcgcc agtcaatttg atttgctcat ccgcatggtc
     1801 gccggataaa gtgcaagttc ggattccgtt aagggcaagg caacaggcga cggagcgcgt
     1861 cgatggaata tgtttttggc atcgcggcat tggtcatcgg atcggtgatc ggctacctgc
     1921 tcggcgagcg gcgaacccgc ggcggggtgg gggaactgcg atcggcggtg gcgtcggcgg
     1981 agcagcgctg cgccggactg gcgtcgcagt tcgatcggca gtccgctgag atcgccgacc
     2041 tgcgcgggca attgtccacc gcggaaaagg aatgcgcttc gatgtccgcg cagctggagg
     2101 gcgctcggca gaacctgctg gagcagcgca agctgctgga cgatgcgaac catcagctga
     2161 agcaggcgtt tgccagcgtc tcggccgaag cattagcgaa gaataacgag gcgtttttgc
     2221 agctggcgaa ggagcgtttt gcgacgctgt ccaccgaagc ggccgggacg ttggagcagc
     2281 gcaaggccca gatcgaaggc ctgctcaagc ccatgcagga actgctgaac cagtaccagt
     2341 cgcggctggg cgacatcgaa aaatcccgag tcgaatcata tagtatgctg cgcgagcagc
     2401 tgggcatcct cacggaaacg cagcgcacgc tgaatacgca aacacatcaa ttggtgagtg
     2461 cgctgcgacg cccgcaaacc cgcggacagt ggggcgaagt gaccttgcgt cggctggtcg
     2521 agctggcggg catggcatct cgctgtgact ttaccgagca gacgacggtc gatacggaag
     2581 atggccggca gcggccggat atggtggtca gcctgccggg ggatcggaca atcgtgatcg
     2641 attgcaaggc gtcgctcgac gcgttcctgg atgccgccgc cgcgccggat gaggacgtgc
     2701 gacgggtgca cctccaaaaa cattgccagc aggtgaagct gcgcgcccgc gagttggcgg
     2761 ctaagagtta ctggtcgcag ttcaagacgt cgcccgagta cgtggtgatg ttcctgccgg
     2821 gcgaagcgtt tctatacgcc gcggtggaac aggacggggc gattattgaa gacacgctca
     2881 agagccgggt gatcatcgcg acaccgacaa cgctgatggc gttgctgcgg gcgatcgagt
     2941 tcggctggcg gcaggaagcg gtgacgcaaa acgcggaaga gatccagaag catgggaagg
     3001 atttgtacga tcggatcgtc gttctcgccc agcatttcac gaaactcggc ggcagcctcg
     3061 acgccgccgt acattcgtac aattccgcga tcggttcgct ggagacgcgc gtgctggtga
     3121 cggcacgcaa ggtggcggag ttgggggcgc gatcggaccg cgaattgccc gagccacaac
     3181 tgatcgacac gcgaccgcgc gagctgggcg ctgctctgca accagcagcc gatccctcct
     3241 agcacgggcg tcttgcccgt gtctggaatc attgggcacg ggcaagacgc ccgtgctaca
     3301 aattttctga tttttcgctg gccgggagcc gaacgcgcgt tatgtttgaa gacgaagagg
     3361 ttcgaatcgg ctgtgcacct cgccgatggg accgcatcca aagacagccc caccggacgg
     3421 tgccaaaact tcagacggga agccgctccg aaacggaagc acgtccccat aatcggctgt
     3481 gactgcttcg agatcgctcg gaacggctag gcgcaaatcg cccgaacacg ctgaagggcc
     3541 ggcgacggca gggatgtcgc ctctgttgct cgatactcat tgtcaaacgc tgtgagtgag
     3601 cccgagcgca catccattgc atcagtcgcc cgaaaaaccc acgagcggat ggcagccaac
     3661 gaaaggtaag gctgcggcga attgcggttt gactgcgcgt gctcgatggc gagcgcgtaa
     3721 gcacaaaatg acgtgccgct gaaacggcat ccgacacgcc cgagagaaga ccaagaaagc
     3781 ccatggcagg attggcgagc gcgcacaaca aacgcggcgg attgtttgac attgattcgc
     3841 cggcaatagc gcgcccgctc ggcttggctg cggtagcacg acccagaagt cggtcggcgt
     3901 cgcgtcgttc gcaatggcgg gtcgcgtgtt ttgaaacacc ttccgcgaat cgggaaggag
     3961 cgcgcgaccg gccgcccgta aaaatcagcg ggtcattggt gcgaggcgcg aagcgcgaaa
     4021 cgacacgtgg ggatgaaccg ccgaaatgcg gggacccgcg gccagggaat actgcgaagg
     4081 gcacttcaaa tcccatgagc gttgccggat cgagcgcgtc ctacaaagtg gcgcgcacgc
     4141 attcggccca gtcgtgaaag ggtctggtga aaatcgggta gcggaacgcc gcgcgaaaga
     4201 gagaccgtgg cgcaatcacc cgcaccgggc cggtgtgaat cgtgaagttc atcgcgcggc
     4261 gtgggccggg cgcacgaagc acacaaggca ataaaaccgc aaggacgacc gtcatcgcct
     4321 cgcaacgtgt accgacttcc ggcgggctgc ggggtgaatg cgaaacgtcg aagcgggatg
     4381 gtccgaaggc tggctgcctc ggattgcgca acacggcctg caggcgctga agggaacaaa
     4441 acctcaagag aggtagagca tgcggatcga tctgccggcc gccgcgaggc gcacggcggt
     4501 tcaatcgatc cggcccgacg aacgcgataa cagagccgac gacgaaaggg tcggccacag
     4561 gtgcgctgaa gcggcaacgc gagcggaaac ccgaacccgc cgggatagcg aacaccagaa
     4621 ccagtcccgc ccggcagccg gtgacgagaa ggctccgcag gagccaatgg tcgatccaag
     4681 ccagcaggac cgacgtcgtc agcaggccat ggcaagggtg atgatccgaa aacgcaagag
     4741 cgcgccggcg tgagaagcag taatgcggct cacgccaatc gcggttgatc gaagacctct
     4801 gaagaggaac aacagttgga aatgactcga gcttcttctc tcacgtttct cttgtaagcg
     4861 gccggccaaa agccggccgc ttgtttttgc gcccgcgttc gatcgcgcgc aggcctgacc
     4921 ggcctgactc gcttcagcga gtcggttc
//
LOCUS       DATIMH010000959         1732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4559621, whole genome shotgun sequence.
ACCESSION   DATIMH010000959 DATIMH010000000
VERSION     DATIMH010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4559621"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VLI90_16655"
     CDS             complement(<1..308)
                     /locus_tag="VLI90_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15895.1"
                     /translation="MPLTLQDKPKKGGSGVGEIRAQVKLTNAVDEALVRRGALRPDQV
                     RRVEVEGVVDTGAVRSVLPIHIVQQLGLQIRGQRMAQYADGREESVGVTEPVIFECLT
                     R"
     gene            complement(327..>1732)
                     /locus_tag="VLI90_16660"
     CDS             complement(327..>1732)
                     /locus_tag="VLI90_16660"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HSV15896.1"
                     /translation="VGLTGRSFIPLLSSFACAIPGIMGTRAITDPRDRLATILVAPLM
                     DALQSAVTWAGSLASSRLPDGPGKALITDGIFKGVGAVVVFVPQIALLFMFLSILEDS
                     GYLARAAFLMDRLLSKVGLHGKSFIPLLSSFACAIPGIMATRTIESRRDRLATILIAP
                     FMSCSARLPVYTLLIGTFFAGLGAFAQAGIMLACYALGILAAVGTAIIFKRTFLKGMP
                     SSFILEMPTYKVPQLSTVARQVWSNASEFLKKAGTIIFALSVVLWALAYYPRLPKAKA
                     DQVEAVVQRDYATNSYRDWEHLLTGQKERLEYSEDAFVKNRVAAAQSEYSFAGRFGHA
                     LAPVIRPLGYNWKMGVGLVAAFAAREVFVSTESIVYATGDTEDTASGGGGTAPLSQAL
                     RADRRPDGTPVWTPLVAISLLVWFVLAMQCMSTVAIVRRETGGWGWPIFMILYMNGLA
                     YVACLVVYQVGTHVLGV"
BASE COUNT          333 a          580 c          527 g          292 t
ORIGIN      
        1 cgcgtgaggc attcaaagat aaccggttcg gtaacaccaa ccgattcctc gcggccatcg
       61 gcatattggg ccattcgttg gccgcggatt tgaagcccca attgttgaac aatgtgaatc
      121 ggcagcacgg agcgcacagc acccgtatcg actacgcctt cgacctcgac ccgacggacc
      181 tgatccgggc ggagcgcccc gcggcggacc aatgcctcgt caacggcgtt ggttagtttc
      241 acctgcgcgc gaatttcacc cacgccggat cctccctttt tcggtttgtc ctgaagcgtc
      301 agtggcatga cctcattata cccagttcac acccccaaaa cgtgcgtccc aacctgatac
      361 acgaccaaac acgccacata cgccaagccg ttcatgtaca ggatcatgaa gatcggccag
      421 ccccagccgc cggtttctct tctgacgatc gcgacggtgc tcatgcattg catcgcgagc
      481 acgaaccaga ccagcaggct gatggcgacc agcggcgtcc acacgggcgt gccgtcgggc
      541 cggcggtcgg cgcgcagggc ctggctcagc ggcgcggtgc cgccgccacc gcttgcggtg
      601 tcttcggtgt cgccggtggc gtagacgatg ctctcggtgc tgacgaacac ctcgcgggcc
      661 gcgaacgcgg ccaccaggcc gacgcccatc ttccagttgt aacccagcgg gcggatcacc
      721 ggcgcgagtg cgtgcccgaa ccggccggcg aagctgtatt cgctctgcgc cgcggcgacg
      781 cgatttttca caaaggcatc ttctgaatat tcgagcctct ctttttgacc cgttaggaga
      841 tgttcccagt ctcggtagct attcgtcgca tagtcgcgtt gcacgaccgc ttcgacctga
      901 tcggccttcg ccttcggcaa ccgcgggtaa tacgccagcg cccacagcac cacgctcagc
      961 gcgaagatga tcgtccccgc cttcttcaga aactcgctcg cgttgctcca cacctgccgg
     1021 gcgacggtgc tcagctgcgg caccttgtac gtcggcattt ccaaaatgaa gctcgacggc
     1081 atccccttga ggaaagtccg cttgaagatg atggccgtgc cgacggcggc caggatgccc
     1141 agcgcgtaac acgcgagcat gatgcccgcc tgcgcgaacg cgccgagccc ggcgaagaat
     1201 gtcccgatga gaagcgtgta aaccggcaga cgcgcgctgc agctcataaa gggggcgatg
     1261 aggatcgtcg ccaatcggtc gcgccggctc tcgatcgtgc gcgtcgccat gatgccgggg
     1321 atcgcgcagg cgaagctcga caacagcggg atgaaactct tgccgtgcag gcccaccttg
     1381 ctgagcaggc ggtccatcaa aaacgcggcg cgcgccaggt acccgctgtc ttccaggatc
     1441 gagaggaaca tgaacagcag cgcgatctgc gggacgaaga cgacgaccgc cccgacgccc
     1501 ttgaagatgc cgtcggtgat cagcgctttc ccgggtccgt cggggagtcg cgaagacgca
     1561 agcgaccccg cccacgtcac ggcggactgg agggcgtcca tcagcggcgc gaccagaatg
     1621 gtcgccagcc gatcgcgcgg atcggtgatc gcgcgcgtgc ccatgatgcc ggggatcgcg
     1681 caggcgaagc tcgacaacag cgggatgaac gagcgcccgg tcagccccac cg
//
LOCUS       DATIMH010000960         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5143327, whole genome shotgun sequence.
ACCESSION   DATIMH010000960 DATIMH010000000
VERSION     DATIMH010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5143327"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VLI90_16665"
     CDS             <1..938
                     /locus_tag="VLI90_16665"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HSV15897.1"
                     /translation="SHSDQQIEWQTGIEDQQPRTGWGGRSADLLYLLNSKNNVSMNIS
                     LSGANQFQVGDTINEYAVSTTGAVSLNIPTTGSGPAQLQALKDIIALNHNNLYQSAFA
                     SKMSTAIDSASQLNNAIAATSASNFFTTPFPNSTLGNQLKMIARLIQAAPTLGHNRQI
                     FFASIGGFDLHSTEGGTSGPQANLLADLSKCMNALYAATTQLNQVNNVTQFTASDFSR
                     TFPVNPTSGTDHAWGNNHLIVGGAVQGQRLYGTYPNLTVGGPDDVSTGRWIPTTSVDQ
                     YSATLAKWFGVSPGNMSSVFPYLGRFATPDLGFMG"
     gene            complement(1115..1348)
                     /locus_tag="VLI90_16670"
     CDS             complement(1115..1348)
                     /locus_tag="VLI90_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15898.1"
                     /translation="MNSANETCRRDVTFHHLFVNQPLAGDEFTIEEMGVQAGTRVTDQ
                     RTGVFYKQGREAQTLRHFTTQAAAEAGGGVDGL"
     gene            1600..2150
                     /locus_tag="VLI90_16675"
                     /pseudo
     CDS             1600..2150
                     /locus_tag="VLI90_16675"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
BASE COUNT          461 a          821 c          700 g          437 t
ORIGIN      
        1 tcagccactc cgaccagcag atcgagtggc agaccggcat cgaggaccag cagccgcgca
       61 ccgggtgggg cgggcgatcc gccgacctgc tctacctgct caacagcaag aacaacgtct
      121 cgatgaacat ctcgctgtcc ggggcgaacc agttccaggt cggcgacacg atcaacgaat
      181 atgccgtctc caccaccggc gccgtgtcgc tgaacatccc gacgaccggc tccggcccgg
      241 cgcagctcca ggcgctgaag gacatcatcg cgctcaacca caacaacctg taccagagcg
      301 cgttcgcctc caagatgtcc accgccatcg acagcgcgtc gcagctcaac aacgccattg
      361 cggcgacctc cgcctccaac ttcttcacca cgccgttccc caactcgacg ttgggcaacc
      421 agctcaagat gatcgcgcgg ctgatccagg cggcgccgac gctcggtcac aaccgccaga
      481 tcttcttcgc gtcgatcggt gggttcgacc tgcacagcac cgaaggcggc accagcggcc
      541 cgcaggccaa cctgctggcg gacctcagca agtgcatgaa cgcgctctac gccgccacca
      601 ctcagctgaa ccaggtcaac aacgtcacgc agttcaccgc gtccgacttc agccgcacgt
      661 tcccggtcaa tcccacctcc ggcaccgacc acgcctgggg caacaaccac ctgatcgtcg
      721 gcggcgccgt ccaaggccag cgcctctacg gcacctaccc caacctgacg gtcggcggcc
      781 ccgacgacgt cagcaccggc cggtggattc ccacaacgtc ggtcgatcaa tattccgcga
      841 cgctcgctaa atggttcggc gtcagcccgg gcaacatgag cagcgtcttc ccgtacctgg
      901 gccggttcgc aacgccggac cttgggttca tgggttgagc gacggctcat catccggcct
      961 ccatgccgaa ttcggaggta cttgcgacga ccgggataac tggacaatcc ccaccgcgcg
     1021 cgttgcgcat gttacgaaat gttacgtttc tcagcgaaaa cgtaacattt atcgtaatgt
     1081 tgctataaac gaggggtcga tctgcctgcg cccattataa accgtcgacg ccgccgccgg
     1141 cttctgcggc cgcctgcgtg gtgaagtgcc tgagcgtttg cgcttcgcgt ccctgtttgt
     1201 agaacacgcc ggtgcgttga tcggtcaccc gcgttcccgc ctgcacgccc atttcttcga
     1261 tcgtgaactc atcccccgcc agcggctggt tgacgaacaa atggtggaac gtcacgtcgc
     1321 ggcggcatgt ctcattggca ctgttcacgg cgtcttgcgt aaacttagaa ggcaaccagg
     1381 cgtccgacct tcgcgtgtgt aatgctgacc gtcgtcgggt gtctgccgcc gtcgtcgaaa
     1441 cagtcgacga cattcccgcc gcgatccagg tctacgctca agctggatag aagggtgtga
     1501 gtggagtcag acgtcgtgca ctggaaaaca atgatcgacc cctggcgcgc aatcttgaac
     1561 tcgcctgcgc agcttcctgg gcgcgatgcc ggcatcagca tgggcaacgc ccgggcgatc
     1621 gcgcagaagt tcaagtggtc cgactacaaa accttcgccg acgtcggcac cgcccagggc
     1681 tgcgtgccgg tgcagttggc gctcgcccat ccgcatctaa acggcaccgg gttcgacctc
     1741 ccggtcgtca agccggtctt tgaggagtac gtcgcgtcgc acaagctaaa cgaccgcgtg
     1801 cggttcgcgc ccggcgattt cttcaagggg aattgccgca ggcggatgtg ctgatcttcg
     1861 gtcacatcct gcacgactgg gatctgccga ccaagaagat gctgctgcgc aaggcgcaca
     1921 acgcgctgcg ccccggcggg gcggtcatcg tgtacgacgc aatgatcgac aacgatcgcc
     1981 gggaaaatgt tttcggattg ctgatgagtt tgaatatgct gatcgagacg ccgggtggat
     2041 ttgattatac tctcgccgac ggcgccgcct ggatgaccgc ggcgggcttc catcacataa
     2101 aggccgaacc gttggccggg ccccattcga tgctggtcgg ctttcgatga gggggctggt
     2161 aaagcgcggg taatcaatgc cggaagtgtt gcgggcgact cgcccggatg atctagcgcc
     2221 aacaccgcgc cacgctcctg gcgtgtcgaa cacggtccat ccctgccgag atcgaccgcc
     2281 gcgtcgatcg cggcaggggg ctgtccacaa catcagctat cgcgccacgc tggtcggctc
     2341 gatggtcacc gtcgtcgtcg ccaccggagc ggctctcacc gctttcgcct ttcacagctc
     2401 gcgcgccaac accgccatg
//
LOCUS       DATIMH010000961         9535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2249211, whole genome shotgun sequence.
ACCESSION   DATIMH010000961 DATIMH010000000
VERSION     DATIMH010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2249211"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VLI90_16680"
     CDS             <1..634
                     /locus_tag="VLI90_16680"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSV15899.1"
                     /translation="PAGSVVSEALLAKNLGISRTPVGEAIRQLWREGLVQQVPRYGTI
                     VKPIERRELIELYEMRAALESYAAGKAAERFEEQTIDRLQEFCDVMHTIAEELESAGV
                     NELDESRLSRFIAADMAFHMTIVDSVGNRRMAESVQTMRTVSRIFRARRVKHDQKVVH
                     RARNYHREILEAIRSRDGERARQKMAEHINISMAESVKHLDQESERYSSS"
     gene            1037..5380
                     /locus_tag="VLI90_16685"
     CDS             1037..5380
                     /locus_tag="VLI90_16685"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HSV15900.1"
                     /translation="MAEEEQLKEQQPAAPIPPDLEPDRMAEPLDWDQPPRLAFPVVGI
                     GASAGGLEAASEFFKVMPSDSGMAFVLIQHLPPQRESMLCEILARRTDMPVHEVTQNL
                     EIQANHLYVIRPGFTLTIKDGRLHLGEPLEKPGHRRPVDDFFRSLAEEQRERAIAIVL
                     SGMGSNGTAGAQAIKAVGGLCIAQDPETAQFPSMARSLIDSGLADFVLKPAEMPDRIQ
                     QFAGHPYVAEPTSAQQILERERTSLNEVLAVLRTRTRQDLSGYKKPTILRRIQRRMGL
                     NQVTRLAEYVQLLRQRPGEATALSDDILIHITGFFRDAAAWENLSTRVIDPMIRNRDD
                     DAPVRVWVTACSSGEEAYTLAMLIAEAMGSFGKHIDVKIFATDTAERTLGHARAGVYP
                     NGIESEISAQRLERFFEKEDSVYRVKKELREMVVFAPQNVLRDPPFSRLDLVTCRNLL
                     IYLEPEMQRRVLSLLHFGLRDGGTLFLGNSETITGLEDMFEPIDKKFRIYKRIGPTRH
                     GEIEFPLPHILVRPDGDNGGGGNGGGNGGRLRPAMRGTQRASVEQMTQRALLDRYTPA
                     AVVVDRQHRVVYYHGKTYRFLSQPTGEPTRDVLTLALEPIRGTLRAALHKISPGLTSP
                     VVVRDGVVEIEGAQRRLEIHLTPIRQRADPAQFFLISFIDLPVEHALSSGDGNGNGNG
                     DGNGNGNGATVTGAGGDVRRLEEELARVRDELQSTIEELQSSNEEMKASNEEITSVNE
                     ELQSTNEELETSKEELQSLNEELTTVNAQLHAKMEELESTTNDLSSLLSSTDIPVLFL
                     DAQFRIRRYTPPVRDLLELIPTDLGRPVSDLAHKFEDEDLLRDAQQVLDRLIPLDKEI
                     LSQSGRSYIRRVLPYRTTDNRINGVVVTFIDITDLRSTQRSLGVAEERRRLALEASGA
                     GDWEYDPRERRFHLSPLVRNLMGMRTGEREEIDVLLNQIHPEDRPEVRAAIEAALDPR
                     GSGIFRVDFRVARASNDFLWVEARGRATFTTDPANRQVAARMRGTMLEITDRKVAEQV
                     LTSARRTAEAANEAKDEFLASISHELRTPLSTILLWTKMLRAGAHKSEELNEALRAIE
                     YSAEAQKQLIEDLLDISRITAGKLRLDLRATDLAQLVRRAVETVRPAAESKALTIQTR
                     IADDIGTVRVDPDRLQQVVWNLLINAVKFTPRGGRIELELQRADQSVRLCVTDSGVGL
                     APSLLPHLFEAFRQGEQSIAPADSTGELSMLQHGGLGLGLSIAKRLVEMHGGTITASS
                     EGVGQGATFTIQLPLEAISAQEATPATKSAGPRSSAKREAATIKALQDNGGQAAQQLA
                     CANVLVVEDVPATRRALEALLQSAGATVTAVETAAAALKAYDQNRPDLIVCDIGLPDV
                     DGFELLRQIRQIESDRGMEPVPGLALTAYTQEKDKRHAIQAGFQQHMSKPPDAVKLIA
                     MLQAMLRQHGR"
     gene            complement(5532..6449)
                     /locus_tag="VLI90_16690"
     CDS             complement(5532..6449)
                     /locus_tag="VLI90_16690"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSV15901.1"
                     /translation="MSDTPLYLGLDIGGTKCAAVAGTAAGEIVDRAEWPSLALRGPAA
                     MLDELFVHARQLMQKHTGMVSIGASIGGPLDAANGIVHSPPNLPGWDAVPLKALIEQQ
                     LGKPARVEHDAAACALAEYRWGAGRGASRLIYLTCGTGFGAGFIIDGAIYRGAGGRSI
                     EIGHAQYRDDGPSAFGKRGSNEAFCAAEALGRLAAWQFPARWPTAPASKTIADLAAAG
                     DADALSVVQLNARAVGDVCATLGDLLRPDVILLGSLARYLGSNWVDAVRQRFRQEVLS
                     DTAAMCRLEPAGLGTKLQDCSALAVAIPR"
     gene            complement(6446..7405)
                     /locus_tag="VLI90_16695"
     CDS             complement(6446..7405)
                     /locus_tag="VLI90_16695"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HSV15902.1"
                     /translation="MTTTSLRVCLVGCLIGIASSLAAAAPSIKTTEGKESLDVTIDGK
                     PFTTYRFVQMADDPEFRRPYFFPVLSADGVPITADRDRESMGQAKREHPWHRSVWIGH
                     GDVNGIDHWSHRTEGERKLQRHLKFTKIDSDGFVEELAWDGNEPGKPVLTETRTVKFI
                     AYDDGARAIDVTSLFTAASGDAVFKVKPLNVSGVEAGWLSARVSSEMAASKESQITSS
                     AGVSGEKDCRSMPANWCDYSGPIGSKTFGVAEFDDPNNPGHPAPWHVRAFGLLTHLGV
                     HDWTLKAGQSQAFHHRLLFHNGDAKSAKLDERYNEFTAAGRGQ"
     gene            complement(7525..7983)
                     /locus_tag="VLI90_16700"
     CDS             complement(7525..7983)
                     /locus_tag="VLI90_16700"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15903.1"
                     /translation="MCHTTGQRFGELLGKLVKLSQHDVEEILEDQTATHRRFGDIALS
                     WGLCEPEHVWQAWSDQLLTQVQRVDLETLGVDSQAAAMIHRDVAQKLCAIPIRCLGKH
                     LIVAIGDNNISRVAAELELITSKQLRFVHADREQIEQAIAAYYPADPKAA"
     gene            8102..8866
                     /locus_tag="VLI90_16705"
     CDS             8102..8866
                     /locus_tag="VLI90_16705"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSV15904.1"
                     /translation="MRIVSYNILDGGEGRADPLAEVILAQRPDVVALVEADDLPTLER
                     IARRLKMDFIHAPAKEHASALLSHWPIVETINHAAVNPEIQKSFLEALVRAPDGRDWP
                     IGVLHLPAQATEAAEEERMHVIPIVLDLFKEHRRANRRHIICGDFNSNAPYQRIDPAR
                     CKIKTQQAWETNGGRLPTRVIEAMTGVGYVDTYRMLHDEQLEMIGSFTTQHPGQRVDY
                     IFAWNVQPADVKDAWVEQDRLAKYASDHFPICAEIT"
     gene            complement(9002..>9535)
                     /locus_tag="VLI90_16710"
     CDS             complement(9002..>9535)
                     /locus_tag="VLI90_16710"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HSV15905.1"
                     /translation="PSVSLLARIALLRRHMPEHPWPTFDDPELGDALAELCANKRSLD
                     EVTRQPLAQAILSRLVYPLDRLLAEHAPESIEVPTGNRVRIDYSGDAPVLAVRLQELF
                     GWTDTPRIAGGRVRVRLHLLGPNYRPVQVTDDLKNFWATTYFQVRKDLRARYPKHSWP
                     EDPLTAKPEAKGSRKRT"
BASE COUNT         1822 a         3171 c         2985 g         1557 t
ORIGIN      
        1 gcccgcgggg tcggtcgtgt ctgaagcgtt gctcgccaag aacctcggca tcagccgaac
       61 tccggtcggc gaagcgatcc gccagctctg gcgcgaaggg ctcgtgcagc aggtgccgcg
      121 ttacggcacg attgtgaaac cgatcgaacg ccgcgagctg atcgagctgt acgagatgcg
      181 cgcggcgctg gaaagctacg ccgcggggaa ggcggcggag cgattcgagg agcagacgat
      241 cgaccggctg caggaatttt gcgacgtgat gcacacgatc gccgaggaac tggaatccgc
      301 cggcgtgaac gaactggatg agtcccgcct cagccgcttc atcgccgccg acatggcgtt
      361 tcacatgacg atcgtggatt cggtcggcaa ccgccggatg gccgagtcgg tccagacgat
      421 gcgcacggtg agccggatct tccgcgcccg gcgggtgaag cacgaccaga aggtcgtgca
      481 tcgcgcccgc aactatcacc gcgagatcct cgaagccatc cgctcgcgcg acggcgaacg
      541 cgcccggcaa aagatggccg agcacatcaa catctcgatg gcggaatcgg tgaagcacct
      601 ggaccaggaa agcgagcgct attcgtcgtc ctgagcgcag cacgtacggg tgcgcggtcg
      661 aaggaacttg ccgtgaatcc aggtgaactt ccactcgcgc aatcccagcc gcttgcggct
      721 tagcatctgt tcaaactaaa aaatgctaag ccgcaaccgg ctgtgacgcg ctgttcgcga
      781 acgagcagtt cacgactcct cggtaagacc cttcgactcc gacccattgt cgcgaacctg
      841 ccaccgctaa gaattcccgc gtttttccgt ggcttgccgc ggtcgattga cttacactca
      901 gggatgtccg agtgcgcgcc gcgcgctgct gctgccagag ttcacacctc acagctcgcc
      961 acgcatactt gatgagcgga cgaaggaagc gcgcgggccc gcgcgttctg ccgagggctt
     1021 gcgcattgag ggattcatgg ccgaagaaga acagctgaaa gagcaacagc ccgccgcgcc
     1081 aatcccgccg gacctcgaac ccgatcgcat ggccgaaccg ctcgattggg accaaccccc
     1141 gcggctggcg tttccggtcg tgggcatcgg cgcctccgca ggtgggctcg aagccgccag
     1201 cgagttcttc aaggtcatgc cgtccgacag cgggatggcg ttcgtcctca ttcagcatct
     1261 gcccccgcag cgcgaaagca tgctctgcga aatcctcgcc cgccgcaccg acatgccggt
     1321 ccatgaagtc acccagaacc tggaaatcca ggcgaaccac ctctacgtga ttcgcccggg
     1381 cttcacgctg acgatcaaag acggccggtt gcacctgggc gaacccttgg aaaagccggg
     1441 gcaccgccgg ccggtcgatg atttcttccg ctcgctggcc gaagagcagc gcgagcgcgc
     1501 gatcgcgatc gtgctctcgg gcatgggcag caacggcacc gcgggcgcgc aggcgattaa
     1561 agccgtcggc gggctgtgca tcgcgcagga tccggaaacc gcccagttcc ccagcatggc
     1621 gcgcagcctg attgactcag gcctggccga cttcgtcctg aagcccgctg aaatgcccga
     1681 ccgcatccag caattcgctg ggcatccata tgtcgccgag cccacctcgg cccaacagat
     1741 cctcgagcgc gagcgcacca gtctgaacga agtgctggcc gtgctgcgca cgcgcacgcg
     1801 gcaggatcta agcggttaca aaaaaccgac gatcctccgc cgcatccaac gccgcatggg
     1861 attgaaccag gtcacccggc tggcggaata cgttcagctc ctgcggcagc ggccaggtga
     1921 agccacggcg ttgtcggacg atatcctcat tcacatcacc ggatttttcc gcgacgccgc
     1981 cgcctgggag aacctgagca cacgcgtgat cgatccgatg atccgcaatc gcgatgatga
     2041 tgccccggtc cgcgtctggg tcaccgcctg ttccagcggg gaagaggcgt acacgctggc
     2101 gatgctgatc gccgaggcca tgggcagttt cggcaagcac atcgatgtca agatcttcgc
     2161 cacggatacc gccgagcgca cgctgggcca cgcccgcgcc ggtgtctatc ccaacggcat
     2221 cgaaagcgaa atttccgcgc agcggctgga acgatttttc gaaaaggaag attcggtcta
     2281 ccgcgtgaag aaggagctgc gcgagatggt cgtcttcgcg ccgcagaacg tgctgcgcga
     2341 cccgccgttc tcccggctcg acctggtcac ctgccgaaac ctgctgatct acctggaacc
     2401 ggagatgcag cggcgcgtgc tgtcgctgct gcactttggc ctgcgcgacg gcggcacgct
     2461 cttcctggga aattctgaaa cgattaccgg cctggaagat atgttcgagc cgatcgacaa
     2521 gaagttccgc atatataagc gcatcggccc gacccgccat ggcgagatcg aattcccgct
     2581 gccgcacatc ctcgtccgcc ccgacggtga caacggcggc ggcggcaatg gcggcggcaa
     2641 cggtgggagg cttcgcccgg cgatgcgcgg cacccagcgc gcttcggtcg aacagatgac
     2701 gcagcgcgcg ctgctcgatc gctacacccc cgcggcggtc gtcgtcgatc gccagcaccg
     2761 cgtcgtctat taccacggaa aaacctatcg ctttctcagc caacccaccg gtgaaccgac
     2821 gcgcgacgtg ctgacgctgg cgctggagcc gatccgcggc acgctgcgcg ccgcgttgca
     2881 caagatcagc ccagggctaa ccagtccggt cgtcgtgcgc gacggcgtgg tggagatcga
     2941 gggcgctcag cgccggctcg agattcacct cacgccgatt cgccagcgcg ccgatcccgc
     3001 ccagtttttc ctgatcagct tcatcgactt gcccgtcgag cacgcattgt cctcgggcga
     3061 cggcaacggc aacggcaacg gcgacggcaa cggcaacgga aacggcgcca ccgtcaccgg
     3121 cgccgggggc gatgttcgcc ggctcgagga ggaactggcc cgcgtgcgcg atgagctgca
     3181 gagcacgatc gaagaactgc agagcagcaa tgaggagatg aaggcttcga acgaggagat
     3241 cacctcggtc aacgaggaac tgcaaagcac caacgaagag ctcgaaacca gcaaggagga
     3301 actgcagtcg ctcaacgaag agctgacgac cgtgaacgcg cagctgcacg cgaagatgga
     3361 agagctggaa tcgaccacca acgacttgag cagcctgctc agcagcaccg acatccccgt
     3421 gctgttcctg gacgcacagt tccgcatccg ccgctatacc ccgccggtgc gcgatctgct
     3481 ggagctgatc ccgaccgacc tgggccgccc ggtcagcgac ctggcgcaca aattcgaaga
     3541 cgaggacctg ctgcgcgacg cccagcaggt gctggatcgg ttgatcccgc tggacaagga
     3601 aatcctcagc cagagcggtc gaagctacat tcgccgggtc ttgccgtatc gcacgaccga
     3661 caaccgcatc aacggcgtgg tcgtcacgtt catcgacatc acggaccttc gctcgacgca
     3721 gcgttccctc ggcgttgccg aggagcgccg ccggctggcg ctggaagcca gcggcgccgg
     3781 cgactgggaa tacgacccca gggaacgccg cttccacctc tcgccgctgg ttcgcaacct
     3841 gatggggatg cgcaccggcg agcgcgagga gatcgacgtg ctgctgaacc agatccatcc
     3901 cgaggaccgg cccgaggttc gcgccgccat cgaggcggcg ctggaccccc gcggcagcgg
     3961 catcttccgc gtcgattttc gtgtggcccg cgcgagcaac gatttcctct gggtcgaagc
     4021 gcgcggccgc gccacgttca ccaccgaccc cgccaatcga caggtcgccg cgcgcatgcg
     4081 ggggacgatg ctggagatca ccgatcgcaa ggtggccgag caggtcctca ccagcgcccg
     4141 ccgtaccgcc gaggccgcca acgaagccaa ggacgagttc ctggccagca tctcccacga
     4201 gctgcgcacg ccgctgtcca cgatcttgct gtggaccaag atgctccgcg ccggcgcgca
     4261 caaaagcgaa gaactgaacg aagcgctgcg cgcgatcgag tacagcgccg aggcgcagaa
     4321 gcagctgatc gaagacctgc tcgacatctc gcgcatcacc gccgggaagc tgcggctgga
     4381 cctgcgggca acggatctgg cccaactggt tcgccgggcg gttgaaaccg tgcgccccgc
     4441 cgccgagagc aaggcgttga ccatccaaac gcgaattgcg gacgacatcg gcacggtgcg
     4501 ggtcgatcct gatcgactgc agcaggtggt gtggaacctg ctgatcaacg ctgtgaagtt
     4561 cacgccgcgc ggcggacgga tcgagctcga actgcagcgc gccgaccaat ctgtccgcct
     4621 gtgcgtgacc gactccggtg tgggcctcgc gccgtcgctg ctgccgcact tgttcgaagc
     4681 gttccgccag ggcgaacaga gcatcgcgcc ggccgactcg accggtgaat tgagcatgct
     4741 acagcacggc gggctggggc tggggttatc gatcgccaag cgactcgtgg aaatgcacgg
     4801 cggcacgatc acggccagca gcgaaggcgt tggtcaaggc gcgacgttta cgatccaact
     4861 gccgctggaa gcgatcagcg ctcaggaggc gacgcccgcg acgaagtcag ccggcccgcg
     4921 gtcgtccgcc aagcgcgagg ccgccaccat caaggcgttg caggacaacg gcgggcaggc
     4981 cgcccagcag ctggcctgcg ccaacgtgct ggtggtggaa gacgtgcccg ccacccgccg
     5041 cgccctggaa gcgctgctgc aaagcgccgg cgccacggtc accgccgtgg aaaccgccgc
     5101 cgcggcgctg aaggcatacg accagaaccg ccccgacctg atcgtctgcg acatcggcct
     5161 gccggacgtg gatggattcg agctgctgcg gcagatccgc cagatcgaaa gcgaccgcgg
     5221 catggaaccg gtgccggggc tggcgctgac ggcatacacc caggaaaagg acaagcgcca
     5281 cgcgatccag gccggcttcc agcaacacat gtccaagccg cccgatgccg tgaagttgat
     5341 cgcgatgctg caggcgatgc tgcggcagca cgggcggtga catcctccca cgccgggttc
     5401 tgttctggtc ccctcccccg gagacctggg ctcagcagta ccgggagagg ggtaggaccg
     5461 aacccggcgc ttgcagtgcc aatatcaatt gtgactgatt tacttcctgc tgctcgccag
     5521 cgtcagcgcg gtcatcgtgg aatcgcgacg gctaacgccg agcaatcctg tagcttcgtc
     5581 ccaagccccg ccggttccaa ccggcacatc gccgcggtgt cgctgagcac ttcctggcga
     5641 aatcgctggc gcacggcgtc cacccaattc gatccaagat atcgggcgag gctgccgagc
     5701 aaaatcacat ccggccgcag caggtcgccc agcgtcgcgc acacgtcacc caccgcgcgg
     5761 gcattcagtt gaacgacgga cagcgcatcg gcgtctcccg cggctgcaag atcggcgatt
     5821 gtctttgacg ccggcgcggt tggccaacgc gctggaaact gccacgccgc caatcggccc
     5881 agcgcctcgg cggcgcagaa cgcttcgttc gatccgcgtt tgccgaaggc gctcgggccg
     5941 tcgtcgcgat attgcgcgtg gccgatctcg atgcttcgcc cgccggcgcc gcggtagatc
     6001 gcgccgtcga tgatgaaccc cgcgccgaac ccggtgccgc aggtgaggta gatcaaccgc
     6061 gacgcgccgc ggccggcgcc ccagcggtac tcggccagcg cgcacgccgc cgcgtcgtgc
     6121 tcgacccgcg cgggcttgcc cagttgctgt tcgatcaacg ccttcagcgg caccgcgtcc
     6181 catcctggta gattcggcgg tgaatggacg atgccgttcg ccgcgtcgag cgggccaccg
     6241 atggatgcgc cgatgctgac catcccggtg tgtttttgca tcagctgccg cgcgtggacg
     6301 aacaactcgt ccaacatcgc cgcgggcccg cgaagggcaa gcgacggcca ctcggcgcga
     6361 tcgacaattt cgcccgcggc ggttccggcg acggcggcgc atttcgtccc gccgatgtcc
     6421 agcccgagat aaagcggcgt gtcactcact gcccgcgccc ggcggcggtg aattcgttgt
     6481 agcgctcgtc cagcttcgct gacttcgcgt cgccgttgtg gaacagcagc cgatgatgaa
     6541 acgcctgtga ctgcccggcc ttcagcgtcc agtcgtgcac gcccaggtgc gtcagcaggc
     6601 cgaacgcgcg cacgtgccac ggcgccggat gtccgggatt gttcggatca tcgaactcag
     6661 cgacgccgaa cgtcttgctg ccgatcggac cggaatagtc gcaccagttg gccggcatgg
     6721 aacggcaatc cttctcaccg ctcacgccgg ccgatgacgt gatctgactt tccttactgg
     6781 ccgccatctc gctcgacacg cgcgctgaca accaccccgc ctcgacgccg ctcacgttca
     6841 gcggcttgac cttgaacacc gcatcgcccg aggcggcggt gaacagcgac gtcacgtcga
     6901 tcgcccgcgc gccgtcgtca tacgcgatga acttcacggt gcgcgtctcg gtcaacaccg
     6961 gtttgcccgg ctcgttgccg tcccacgcca gctcttcgac gaatccgtcg gaatcgatct
     7021 tggtgaattt caggtgccgc tgcagcttcc gctcgccctc ggtgcgatgg ctccagtggt
     7081 cgatgccgtt gacgtcgccg tggccgatcc agacggaccg atgccacgga tgttcgcgtt
     7141 tcgcctgccc catcgactcg cgatcgcgat ccgcggtgat cggcacacca tcggcagaaa
     7201 ggaccggaaa gaaatacggc cggcgaaatt ccggatcgtc ggccatctgc acgaagcggt
     7261 aggtggtgaa gggcttgccg tcgatcgtga cgtcgagcga ttctttgccc tcggtggtct
     7321 tgatggaagg cgccgccgcg gcgagggatg aggcgatgcc gatcaagcac ccgacaaggc
     7381 aaacccgaag cgaggtggtt gtcatggccg aggattgtac cggctcaccg aggcatttgg
     7441 atgtagaacg atgaatgcag aatgcagaat gaacacggaa gcgattcctc cattctgcat
     7501 tcatcattct gcattgatca tccgctacgc cgccttggga tctgccggat aatacgccgc
     7561 gatggcctgt tcaatctgct cgcgatcggc gtgcacaaat cgcagctgct tcgacgtgat
     7621 caactccagc tccgccgcga cgcgcgagat gttgttgtcg ccaatggcga cgatcagatg
     7681 cttgcccagg cagcggatcg gaatcgcgca tagtttttgc gcgacatcgc gatggatcat
     7741 cgccgccgcc tgggaatcga cgccgagcgt ttccagatcg acgcgctgca cctgcgtgag
     7801 cagttgatcg gaccacgcct gccagacgtg ctcgggctcg cacaaacccc acgatagcgc
     7861 aatgtcacca aaccgccgat gcgtggcggt ctggtcttcg agaatctcct cgacgtcgtg
     7921 ttggctgagt ttgacgagct taccgagtag ctcaccgaat cgttgacctg tcgtatggca
     7981 catacccgct cctcctgcta tcacaaacta cgatgcgaat gacccgcggg caaaacgcaa
     8041 aattgctcgt tacagccggt aacactttgg ccaataatgt acgggaaatc aggcggttct
     8101 catgcgaatc gtgagctaca acattttgga cggcggcgaa ggccgggctg atccgctggc
     8161 ggaagtaatc ctggcgcagc ggccggatgt cgtcgcgctg gtggaagcgg atgacctccc
     8221 gacgctggag cgcatcgccc ggcgattgaa gatggatttt atccacgcgc cggcgaagga
     8281 gcacgcatcg gcgctgctga gccactggcc aatcgtcgag acgatcaatc acgccgcggt
     8341 caatccggag atccagaagt cgtttctcga agcgctggtg cgcgcgcccg atggccgcga
     8401 ttggccgatc ggtgtgctgc acctgccggc gcaggcgacg gaggcggcgg aagaggagcg
     8461 gatgcacgtg atcccgatcg tgctcgacct gttcaaggaa caccgccggg ccaaccgccg
     8521 gcacatcatc tgcggcgact tcaactccaa cgcgccgtac cagcgcatcg atcccgcgcg
     8581 ctgcaaaatc aaaacacaac aagcctggga aaccaacggc ggccgattgc cgacgcgcgt
     8641 gatcgaagcg atgaccggcg tcggttacgt cgatacctat cgcatgctgc acgatgagca
     8701 actggagatg atcggctcgt tcaccacgca acaccccggc cagcgcgttg actacatctt
     8761 cgcgtggaac gtgcagccgg ccgacgtgaa ggacgcgtgg gtggagcagg atcgcctggc
     8821 gaaatacgcg agcgatcatt ttccgatttg cgccgagatc acctgagaaa ccgcgccagc
     8881 atagagccca gcggctccgc cgcgggacag gagtgaaatt gcgcattttt tgcgagtgac
     8941 gcaactcgaa tcgcgccccg cggtagagcc gctgggctct cggctggcgc gcggattgac
     9001 gttatgtccg ctttcgcgag ccttttgctt cgggcttggc cgtgagcggg tcctccggcc
     9061 acgaatgctt cgggtacctc gcccgcaaat ccttccgcac ttgaaagtac gtcgtcgccc
     9121 aaaaattctt cagatcgtcc gtcacctgca ccggccgata gttcggcccg agcagatgca
     9181 gccgaacccg cacgcgtccg ccggcgatgc gtggcgtatc ggtccagccg aacaactcct
     9241 gcaaccgcac cgccagcacc ggggcatcgc cgctgtagtc gatgcgcacg cgattgcccg
     9301 tcggcacttc gatcgactcc ggcgcatgct cggccagcag tcgatcgagc ggatacacga
     9361 gccgcgacag gatcgcctgc gccagcggtt gccgcgtgac ttcgtccagc gatcgcttat
     9421 tcgcgcacaa ttccgccagc gcatcgccca attccggatc gtcgaatgtc ggccaagggt
     9481 gttcgggcat gtgccggcgc agcagcgcga tccgcgccag cagcgacacc gacgg
//
LOCUS       DATIMH010000962         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3696209, whole genome shotgun sequence.
ACCESSION   DATIMH010000962 DATIMH010000000
VERSION     DATIMH010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3696209"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="VLI90_16715"
     CDS             <1..1208
                     /locus_tag="VLI90_16715"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HSV15906.1"
                     /translation="QLDALGLPIIVINDGSDDDTAGTLGTWADAAKGRFVETHPTNRG
                     KAAALLSGFDCARAHGFTHAVTVDTDLQHDANDVPPLLALSRQHPASLVLGVRPITTD
                     DYPAASRVGRGISNLLVLIECGVRVADSQCGLRVYPLVLMAKVKTTSGRYGFETEIIT
                     RAAWAGIDICETDVRCIYDVPQGRVSHFRPVMDSVRAVGTHLRLLARSLLPWPTIKVQ
                     QDNPQAGTILEQLLRWLNPAKAWRDIRAGRGDGRFPASVAAGMFMATVPLYGVKTVVC
                     LAIAKVLRLQPLVVLAVSSLNTPPVGPLLAMLSIVTGHALLHQELPSWPQADVLHQLR
                     WNVLGAVAWEWVLGSIVCGTVLAIIAYFAARGFVRLAARDDQVMSGRQSVATAASPAT
                     EPAMCRPHS"
     gene            complement(1132..>2302)
                     /locus_tag="VLI90_16720"
     CDS             complement(1132..>2302)
                     /locus_tag="VLI90_16720"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSV15907.1"
                     /translation="GAAAVVLLLICATFARGIHVETESDPMVLHPRPSPPLEAQQRIA
                     RKMQTGAGSILVHLQAPTDEQLLALAHVVHERLRDAAVTSAGVAGVFGLDTLLPDPRV
                     VTARAKAFDPGFADRAAQDFREVLAQSDFKPDAYDSYIDFIKHLASPGPPPAVATVSD
                     YPELAHLLLPRQSSAGSPRTEALELIFPRTPLETREARDAILRAVRDALANVDGATVT
                     GTVAIGHDLEQAVHRDLPRSIVIALVCIALYLELHFRSIRLSLLALLPIVISLMFVVA
                     CFCLAGAKLNLLHTVMAPLLLGINLDYGIFAVHAWRTSRDETELSHRFTPAFAALLLC
                     GGATVIGFGSLILTSIPAVKSLGWLINVGIISCVAGTLLVQWPVMLLLRPTGDRT"
BASE COUNT          421 a          782 c          714 g          385 t
ORIGIN      
        1 cgcagctgga tgcgctgggg ctgccgatca tcgtcatcaa cgacggctcg gacgacgaca
       61 ccgccggcac gctcggcact tgggcagacg cggcgaaagg ccgcttcgtc gaaacccatc
      121 caaccaaccg cggcaaagca gccgcacttt tatccggctt cgactgcgca cgagcgcatg
      181 gcttcactca cgccgtcaca gttgacaccg accttcagca cgatgcgaac gatgttcccc
      241 cgctgctggc gttgtcgcgg caacaccccg catcgctcgt cctgggcgtg cgaccgatca
      301 ccaccgacga ctatcccgcg gccagccgcg tcgggcgcgg catctcgaat cttcttgtgc
      361 taatcgaatg cggcgttcgc gtcgccgaca gccagtgcgg actgcgcgtc tatccgctgg
      421 tgttgatggc gaaggtgaag accacgtcgg gccgctatgg atttgaaacc gagatcatca
      481 cgcgagccgc gtgggcgggc atcgacattt gtgagacgga cgttcgctgc atctacgatg
      541 tgccgcaggg ccgcgtgtcg cattttcggc cggtgatgga ttccgttcgc gccgtcggca
      601 cgcacctgcg attgctggcc cgatcactgc tgccttggcc caccataaag gtgcagcaag
      661 ataatccgca agccggcacg atcctcgagc aattgctgcg atggttgaac cccgccaaag
      721 cctggcggga catccgcgcc ggccgcggcg acggccggtt tcctgcgtcg gtggccgccg
      781 ggatgttcat ggcgaccgtg ccgctgtacg gtgtgaagac cgtcgtctgc ctcgccatcg
      841 caaaggtgct tcgcctgcaa ccgctggtcg tcttggccgt ctccagctta aatacgccgc
      901 cggtgggccc gctgctggcc atgctgtcga tcgtcaccgg gcatgcgctt ctgcaccagg
      961 agttgccgag ttggccgcag gccgacgtgc tgcatcaatt gcggtggaac gtgctgggcg
     1021 cggtcgcgtg ggagtgggtg ctcggcagca tcgtctgcgg cactgtgctg gcgatcatcg
     1081 cgtatttcgc cgcgcgcggg ttcgtccgat tggcggcgcg cgacgatcaa gtcatgtccg
     1141 gtcgccagtc ggtcgcaaca gcagcatcac cggccactga accagcaatg tgccggccac
     1201 acagctgatg atgccgacgt tgatcagcca ccccagcgac ttgaccgccg ggatgctggt
     1261 gaggatcagc gagccaaatc cgatgaccgt cgccccgccg cacaagagca acgccgcgaa
     1321 cgccggcgtg aagcgatgcg ataattccgt ctcatcgcgc gaggtccgcc acgcgtgcac
     1381 cgcaaagatg ccgtaatcca ggttgatccc cagcagcagc ggcgccatca ccgtgtgcag
     1441 caggttcaac ttagccccag ccaagcaaaa gcacgcgacg acgaacatca ggctgatcac
     1501 gatcggcagc agcgccagca gcgacaggcg gatcgaacgg aaatgcagct ccaggtaaag
     1561 cgcgatgcac accagcgcga tcacgatcga ccgcggcaag tcgcggtgca cggcttgttc
     1621 gagatcgtgc ccgatcgcca ccgtgccggt caccgtcgcg ccatcaacgt tggccagcgc
     1681 atcgcgaaca gcgcggagga tcgcatcccg cgcttcgcgc gtctccagcg gcgtccgcgg
     1741 aaagatcaac tccagcgctt cggtcctcgg cgaccccgcc gaactttgcc gcggcagcag
     1801 tagatgggct agctccggat aatccgacac cgtcgccacc gccggcggcg gccccggtga
     1861 cgcgaggtgc ttgatgaaat cgatatacga atcgtacgca tcgggtttga agtcactctg
     1921 cgcaagcact tcgcgaaaat cctgcgccgc tcgatccgca aaaccgggat cgaacgcttt
     1981 ggcgcgcgcc gtcacaaccc gcggatccgg cagcagcgtg tccagcccga acacaccagc
     2041 cacaccggcg ctcgttaccg ctgcatcccg cagccgctcg tgcacgacgt gcgccagcgc
     2101 gagcaactgc tcatcggtcg gcgcctgcag atgaacgagg atcgatcccg cgcccgtctg
     2161 catttttcgc gcaatccgct gctgcgcctc cagcggcggg cttggccgcg ggtgcagcac
     2221 catcggatcc gattccgtct cgacatggat gccgcgggcg aatgttgcgc agatcagcag
     2281 cagaacgacg gcagccgcgc cg
//
LOCUS       DATIMH010000963         9401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2539328, whole genome shotgun sequence.
ACCESSION   DATIMH010000963 DATIMH010000000
VERSION     DATIMH010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2539328"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /gene="carB"
                     /locus_tag="VLI90_16725"
     CDS             complement(<1..1469)
                     /gene="carB"
                     /locus_tag="VLI90_16725"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSV15908.1"
                     /translation="MPRRTDIHTILIIGSGPIVIGQGCEFDYSGVQACRALREEGYRI
                     VLINSNPATIMTDPEFADATYVEPITPEAVEKILAYEKQQGTPIDAVLPTLGGQTGLN
                     TAMACYDRGIFTKFGVQMIGANRDAIHRGEDRQVFKDLMIQIGLKVPISRVVHNMADA
                     REAMDAIGLPLIIRPAFTLGGTGGGIAYNVEEFETIVQRGLDASPVTEVLIEQSVIGW
                     KEFEMEVMRDKNDNVVIICSIENLDAMGVHTGDSITVAPIQTLTDKEYQRMRDASIAV
                     IRAVGVETGGSNIQFAINPDNGDMIVVEMNPRVSRSSALASKATGYPIAKLAAKLAVG
                     YTLDELPNYITSRKRASNPASQVPGDISKASKTISPASKMILDAPKMVFEAPKTTSDA
                     AKMISEAPKTTSDAAKMISEAPKTILHAPKIVSDAPKMISQGSETTSRTSSSVREDYD
                     LYTSACFEPTIDYVVIKIPRWTFEKFPDADETLTTQMKSV"
     gene            complement(1650..2249)
                     /locus_tag="VLI90_16730"
     CDS             complement(1650..2249)
                     /locus_tag="VLI90_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15909.1"
                     /translation="MSVTVDQEPLAAEALGLVTVGQVLAHLQRDDRLVVNLLIDGEQP
                     DLSRMGALRQSSVNGRTLYIETADPSEMALEALDEVEEQLKSADKFKNDAAELLQRNQ
                     VARAMEKLGQCFTTWQAGQESVLKVGQLLRLDLEAMSIGGGQSLIDVLGEFTQQLRDI
                     KDTLVERDFVALTDLLLYETTETNLRWSAVLDALRAVIS"
     gene            complement(2328..2993)
                     /locus_tag="VLI90_16735"
     CDS             complement(2328..2993)
                     /locus_tag="VLI90_16735"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSV15910.1"
                     /translation="MAPSTMTAREARIPHGRLTLDGDLVVPDHASGVVLFAHGSGSSR
                     FSRRNRYVAGDLQELGLATLLMDLLTADEERIDQVTAHLRFDIHLLADRLVAATDWVR
                     AQPEMSNLPVGYFGASTGAGAALVAAARPEMVAAVVSRGGRPDLAGDALPAVRAPTLL
                     IVGGRDVQVIALNRTAMQQMRCEKQLTIVPGATHLFEEPGALEEVARLAGDWFQQKLA
                     TNP"
     gene            complement(2997..3275)
                     /locus_tag="VLI90_16740"
     CDS             complement(2997..3275)
                     /locus_tag="VLI90_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15911.1"
                     /translation="MSARIVTLPELGLISGTRAALGVGVGLLVADKLTSEQRRAVGWT
                     LVAVGVITTFPLAAQVFLRENPSDLLPRQGRGGERGQREFAGTTAARS"
     gene            complement(3272..3703)
                     /locus_tag="VLI90_16745"
     CDS             complement(3272..3703)
                     /locus_tag="VLI90_16745"
                     /inference="COORDINATES: protein motif:HMM:NF036463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HSV15912.1"
                     /translation="MTTYEVPPTEWHSFFDQFSRLHQGQPVDVQTVDSSGRQRSNTHG
                     VPLLGITAETAPSAPASPNHGRLDISAGDERGVQIGHSVTEPARVKVSEWNDGLSASL
                     EIESGGGLTTRVRVGPVEEMLPPGFILDGIESHPLGKDGRS"
     gene            complement(3740..5113)
                     /locus_tag="VLI90_16750"
     CDS             complement(3740..5113)
                     /locus_tag="VLI90_16750"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HSV15913.1"
                     /translation="MRISTANIIRGIRSSARPLEGAHDGHAAPAYDPLIDAIGDRRFA
                     LIGEASHGTHEFYATRAAITRRLIAERGFNAVVVEADWPDAYRVNRYVRGFNDDSSAD
                     GALSGFRRFPQWMWRNTVVEEFVDWLRAYNAAASAPRAQVGLYGMDLYSLRASIAAVL
                     RYLDNVDHAAATRARYRYSCFEDFGEDPQAYGYAARFDLERSCEEAVVQQLVELQRRS
                     AEYARRDGRAAEDEYFHAEQNARLVKNAEQYYRSMFQGRVSSWNLRDTHMVQTIGELV
                     RFLDRMNGAPTKLVIWAHNSHLGDASATEVGQAGELNVGQLMRETYGQEQTFLTGFTT
                     HDGTVTAATDWDGPAETKTVRPSLDGSYEQLFHETQLPRFLLILSGGGADRSMIEPLR
                     ASRLERAIGVIYRPQTERVSHYFHAILPEQFDAIIHLDRTRAITPLEAVATVVDRELE
                     ETYPTGV"
     gene            complement(5139..5819)
                     /locus_tag="VLI90_16755"
     CDS             complement(5139..5819)
                     /locus_tag="VLI90_16755"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HSV15914.1"
                     /translation="METRFKNRQQAGQFLGDLLSKRLTDSNDVIVLALPRGGVPVGYE
                     VAQRLGAPLDVFVVRKLGVPGHEELAMGAIASGGVRVLNQRVVQFMPINQAIIERVVA
                     RETEELRRREQLYRGDRPPPEVEGKTVILIDDGLATGATMRAAAEALRQRRPRRLILA
                     VPVAAPETCEEFRGVVDEIVCGRTPEDFVAVGMWYQDFSQTTDEEVRELLQHNAAEHP
                     APTAAAHS"
     gene            5974..7407
                     /locus_tag="VLI90_16760"
     CDS             5974..7407
                     /locus_tag="VLI90_16760"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSV15915.1"
                     /translation="MSDREHFEQLILAHHPCVVVATVDEAYVLTLLREIAVQAQREMW
                     QWSFTNGLGDALLSDSPTIADSEHPAGALYYLINNVTRSIICALLDLPGHLKDDRVLR
                     LLRETIDHFGRIGGMLVLIHPSADLPPAIAATTFEVSFPDKVELEGILRETSRKLASE
                     RQITVNLSRQGLNAIVRNLQGLSRSQARRVLTDAICEDCRLDEHDVNAVLAGKRKALS
                     GAGMLQYVESPVDLNEVGGLEKLKQWVVQRRPALENEAAIEFGLTPPRGVLMLGVQGA
                     GKSLAAKAVATAGQLPLLRMDVGSLYDPYIGQSERRLRDALRQAELMSPVVLWIDEIE
                     KAFASAASESTDGGLSRRMFGALLTWMQERTAPVFLIATANDIEALPPELLRKGRFDE
                     IFFVDLPSLESRTQIVAIHLRKRKRDPAKFDMATIADAAAGYSGAEIEQAIVGALFDA
                     FDAKRELTTADVVASLKRSPPLSVTMA"
     gene            7661..9301
                     /gene="serA"
                     /locus_tag="VLI90_16765"
     CDS             7661..9301
                     /gene="serA"
                     /locus_tag="VLI90_16765"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HSV15916.1"
                     /translation="MALKILVADKLAEEGLEFLKSSGIDFDVKVGLSDDELAAVAGNY
                     DAMIARSAPKKSAISKALANPGHLKAIARAGVGVDNIDLEAATAKGILVLNTAEASTL
                     STAEHALALMLSLARKIPQAHAALKSQQLDWKKRNTFQGTQLAGKTLGVVGLGRIGRT
                     VATRALAMEMNVVAYDPFFSAPTALEGRVKLYKDFDEFLSNIDVITFHVPGGEATKHL
                     LNRERLFNKARPNLLVVNDARGEVVDEYALADALKEKRIAGAAIDVFAVEPPPKDHPL
                     FGLDNVVLTPHLGASTDEAQTAVSVDAAKAIVAYLYRGEIRGAVNAGGIRLDLPPDEA
                     PFAELARRIGRLLAGVTDSGIKTITLRASGTRAPRLMNTLLRLAAVELLKPHLGEGSV
                     NVINVDHLARGRGIEMVQVHEPAPPSGLVGDILGVRVDGPNGESHRILGTVYADGLPR
                     VLRVDDYAMDMIPEGSMVIIINEDQPGVIGTVGTAFGDAKVNIADMVISRAFNDQGKA
                     TALMVIKTDSEAPDDLLNKLRARPGIVRAKSVKLPPRG"
BASE COUNT         1716 a         3052 c         2908 g         1725 t
ORIGIN      
        1 acgctcttca tctgcgtcgt cagcgtttcg tcagcgtccg ggaatttctc gaatgtccag
       61 cgtgggatct tgatgacgac gtagtcgatt gtcggctcga agcaggcgct ggtgtacagg
      121 tcgtagtctt ctcggaccga actggacgtc cgcgaagtcg tttccgagcc ctgcgaaatc
      181 attttcggtg cgtcggaaac gattttcggt gcatgaagaa tcgttttcgg ggcctcggaa
      241 atcattttcg ctgcatcgga agtcgttttc ggggcctcgg aaatcatttt cgctgcatcg
      301 gaagtcgttt tcggtgcctc gaaaaccatt ttcggtgcat ccaaaatcat tttcgatgca
      361 ggagaaatcg ttttggatgc cttggaaatg tctcccggaa cctgcgatgc ggggttagaa
      421 gcgcgtttac gggacgtgat gtagttcggc agctcgtcca gcgtgtaccc gaccgccaat
      481 ttcgcggcca gtttcgcgat cggatatccc gtcgctttgg aagccagcgc ggatgagcgc
      541 gacacgcgcg ggttcatctc gacgacgatc atgtcgccgt tgtcggggtt gatggcgaac
      601 tggatgttgc tcccgccggt ttccacgccg accgcgcgga tgaccgcgat gctcgcgtcg
      661 cgcatgcgct gatattcttt atccgtcagc gtctggatcg gcgccaccgt gatggaatcg
      721 ccggtgtgga cccccatcgc gtctaaattc tcgatcgagc agatgatgac gacgttgtcg
      781 ttcttgtcgc gcatcacctc catctcgaat tctttccagc cgatgaccga ttgctcgatg
      841 agcacttcgg tgaccggcga ggcgtcgagg ccgcgctgca cgatggtttc gaattcttcg
      901 acgttgtagg cgatgccgcc gccggtgccg ccgagcgtga acgcggggcg gatgatcagc
      961 ggcaggccga tcgcgtccat cgcctcgcgg gcatcggcca tgttgtgaac gacccggctg
     1021 atcggcacct tcaggccgat ctggatcatc aggtccttga acacctgccg gtcttcgccg
     1081 cggtgaatcg catcgcgatt ggcgccgatc atctgcacgc cgaacttggt gaagatcccg
     1141 cgatcgtagc aggccatcgc ggtgttcagg ccggtttgtc cacccaatgt gggaagcacc
     1201 gcgtcgatgg gcgtcccctg ctgcttctca tacgccagga tcttttccac cgcctccggc
     1261 gtgatgggtt cgacgtaggt cgcgtcggcg aattccggat cggtcatgat cgtcgcgggg
     1321 ttggagttga tcaggacgat gcggtaaccc tcttcgcgca gcgcgcggca ggcctgcacg
     1381 ccggaatagt cgaattcgca gccctgaccg atgacgatgg gaccggagcc gatgatgagg
     1441 atggtgtgga tgtcggtgcg tcgtggcatt tcgttgtgcg caatcgtaag cgtgctgtag
     1501 tcgagccgcg cgggggccgc ggaaagaaat tcgcggcagt gtaatgatcg gggcgggttt
     1561 ttcaaaagcg cgaagcgcgc gcgatgacgc cggtgtctga ggtactccga agacccggcg
     1621 tcggtagcag tttgtcgaaa gagaggtgct taagagatga cagcgcgcag cgcatccagc
     1681 accgcagacc aacgcagatt cgtttccgtc gtctcgtaca gcagcaggtc cgtcagcgcg
     1741 acgaagtcgc gctcgaccag cgtgtccttg atatcccgca gttgctgcgt gaattcgccg
     1801 agcacgtcga tcagcgattg tccgccgccg atcgacattg cttccaggtc caagcgcagc
     1861 agttgcccca ccttcaatac cgattcctgg cccgcctgcc aggtcgtgaa gcactggccc
     1921 agtttttcca tcgccctggc gacttgattg cgctgcagaa gctcggccgc gtcgttcttg
     1981 aatttgtccg cgctcttcag ctgctcttcc acctcgtcca acgcttccag cgccatctcg
     2041 ctggggtcgg ccgtctcgat gtacagcgtg cgcccgttga cggacgattg ccgcagcgcc
     2101 cccatgcgcg acaggtccgg ctgctcgccg tcgatcagca ggttgacgac caaccggtcg
     2161 tcgcgctgca ggtgagccag cacctgccca acggtcacga gccccaacgc ctcggcggcc
     2221 aacggttcct gatcgacggt gacggacatg cgatacgctc ccgggatgaa tgcccgatcg
     2281 agttatcggt ccttcatcca ccaaagcttg aagcgcgcat ccgataatca tggattggtg
     2341 gccagctttt gctgaaacca gtcgccggcc agccgggcga cctcctccag cgcgccgggc
     2401 tcttcgaaca agtgcgtcgc gccagggacg atcgtcagct gtttttcgca gcgcatctgc
     2461 tgcatcgccg tgcggttcaa cgcgatgacc tggacgtcgc gcccgccgac gatcagcagg
     2521 gtcggcgcgc gcacggcggg caaggcatcg ccggccaggt cgggtcggcc gccgcgcgac
     2581 accaccgccg ccaccatctc cggtcgcgcc gccgccacca acgcggcccc agcgccggtg
     2641 ctggcgccga aatatcccac cggcaggttg ctcatctccg gctgggcgcg cacccaatcc
     2701 gtcgccgcca ccagccgatc cgccagcaga tggatgtcga aacgcaggtg cgccgtcacc
     2761 tggtcgatcc gctcctcatc cgccgtcagc aggtccatca acagcgtagc caaacccaat
     2821 tcctgcaagt cgccggcgac atagcggttg cgccggctga accggctgct gccgcttcca
     2881 tgtgcgaata gcaccacgcc gctggcgtgg tcgggaacga ccagatcgcc atcaagtgtc
     2941 aaccggccgt ggggaatgcg cgcctcgcgc gccgtcatcg ttgagggtgc catgagtcaa
     3001 ctccttgcgg cggtagtgcc ggcaaactcg cgctggcctc gctcgccgcc gcgtccctgc
     3061 cgcgggagta agtccgacgg gttctcccgt aaaaatacct gcgccgccag cggaaatgtc
     3121 gtgatcacgc cgaccgcgac cagcgtccaa cccaccgccc gccgctgctc gctggtcaat
     3181 ttgtcggcca cgagcaagcc aacgcccact cccagcgccg ctcgtgtgcc gctgattaaa
     3241 cccagctccg gcaatgtcac gattcgtgca ctcatgagcg gccgtccttt ccaagtggat
     3301 gactctcaat cccgtccaga atgaatcctg gcggcagcat ttcctcgacc ggtccaaccc
     3361 gcacgcgcgt cgtcagaccg ccgccggatt cgatctccag cgacgccgac aatccgtcgt
     3421 tccattccga caccttcacg cgggcgggtt cggtcacgga atggccgatc tgaacgccgc
     3481 gttcgtcacc ggcgctgatg tccagccgcc catgattcgg cgatgccggc gccgacggcg
     3541 ccgtctcggc ggtaatgccg agcagcggaa cgccgtgcgt attcgaacgt tgccggccgc
     3601 ttgaatcgac cgtctggacg tcgaccggct gtccctggtg gagccggctg aactggtcga
     3661 agaacgagtg ccattcagtt ggagggactt cgtacgttgt catatcgatc acctccgggc
     3721 gcgcgaggcg agcatgcgct cacacgccgg ttggatacgt ttcttccagt tcgcgatcga
     3781 ccaccgtcgc caccgcttcc aacggcgtga tcgcccgcgt gcgatccaga tgaatgatgg
     3841 cgtcgaattg ctcgggaaga atcgcgtgga aatagtggct gacccgctcg gtttgcggtc
     3901 gatagatcac gccgatcgcc cgctccagcc ggcttgcccg caacggctcg atcatcgatc
     3961 gatcggcgcc gccgcccgac aggatcagca ggaaccgcgg caactgtgtt tcgtgaaaca
     4021 actgttcgta gctgccgtcc agcgaaggtc gaaccgtctt cgtctccgcc ggcccgtccc
     4081 aatcggtcgc ggcagtcacc gtgccatcat gcgtggtaaa accggtcagg aacgtctgct
     4141 cctgcccata tgtttcgcgc atcagttggc cgacgttcag ctcgcccgcc tgacccactt
     4201 cagtggcgct ggcgtcgccc aggtgcgagt tgtgcgccca gatgaccagc ttggtgggcg
     4261 cgccgttcat ccggtccagg aaacgaacca gctcgccgat cgtctgcacc atgtgcgtgt
     4321 cgcggaggtt ccacgacgag acgcggccct ggaacatcga gcggtaatac tgttccgcgt
     4381 tcttcaccag ccgggcgttt tgctcggcgt ggaagtactc gtcctccgcc gcgcgaccat
     4441 cgcggcgggc gtattcagca gaccggcgtt gcagttccac cagctgctga accaccgcct
     4501 cttcacacga ccgttccaaa tcgaaccgcg ccgcgtagcc gtacgcttgc gggtcttcac
     4561 cgaagtcctc gaagcacgaa tagcgatagc gcgcccgagt cgcggcggca tgatcgacgt
     4621 tgtcgagata ccgcaacacc gcagcgatgg atgctcgcag gctgtagagg tccatcccgt
     4681 aaaggccgac ctgcgcccgc ggcgcgctgg ccgcagcgtt gtatgcccgc agccaatcga
     4741 caaattcttc gaccactgtg ttgcgccaca tccactgcgg gaaccggcgg aatcccgaca
     4801 gcgctccgtc cgcgctgctg tcgtcgttga acccgcgcac atatcgattg acgcgatagg
     4861 catccggcca gtccgcctcg accacaacgg cgttgaaccc gcgctcggcg atcaaccgcc
     4921 gggtgatcgc ggcacgcgtc gcataaaact cgtgcgtgcc gtgcgacgct tcaccaataa
     4981 gcgcgaagcg gcgatcgccg attgcatcaa tcaatgggtc gtacgcgggg gcggcatgac
     5041 cgtcatgggc gccctcgagc ggacgggcgc tcgaacggat tccgcgaatg atgtttgcgg
     5101 ttgaaatacg catgatctta aggcctcttc tccgcgcgct aagaatgcgc ggctgccgtc
     5161 ggcgcgggat gttccgcggc gttgtgctgc agtaactcgc gcacctcttc atccgtcgtc
     5221 tgggagaaat cctggtacca catgccgaca gcgacgaaat cctccggggt tcggccgcag
     5281 acgatttcgt cgacgacacc gcggaactcc tcgcacgtct ccggcgccgc caccggcacc
     5341 gccaagatca accgcctggg ccgccgctgc cgcagcgctt cggccgccgc ccgcatcgtt
     5401 gcgccggtcg ccaagccatc gtcaatcaaa atgacggttt tgccttccac ctccggtggc
     5461 gggcgatccc cgcggtagag ttgctcgcgc cgacgcaatt cttccgtctc gcgcgcgacg
     5521 acccgctcga ttatcgcctg gttaattggc atgaactgaa cgacgcgctg gttgagcacg
     5581 cgaaccccac cgctggcgat cgcgcccatc gcaagctctt catgccccgg cacaccgagt
     5641 ttccgcacga cgaacacatc gagcggcgcg ccaagccgct gcgccacctc atacccgacg
     5701 ggaacgccgc cacgcggcag ggcaaggaca atcacgtcat ttgaatcggt caatcgcttt
     5761 gacaacaagt cccccaggaa ctgccccgct tgctgacgat ttttgaatcg agtttccatg
     5821 atccgcctcg caagctcatc aaacgcggtg atgcagcaac gcgagcacca tttccatgat
     5881 gccgcaatct gaataccgat gacggccaaa tccgccgtaa acaacgcgaa aagcttgatc
     5941 gcgttccaac aatggttgag actctctttt tgcatgagcg accgcgagca tttcgagcag
     6001 ttgatcctgg cgcaccaccc gtgcgtcgtg gtcgcgacgg tggacgaggc gtacgtgctg
     6061 accctgctgc gcgagatcgc ggtccaagcg cagcgagaga tgtggcaatg gtcgttcacc
     6121 aacggcttgg gtgacgcgct gctgtccgat tcccccacca tcgccgacag cgagcaccct
     6181 gctggcgcgc tgtactacct gatcaacaac gtcacccgat ccatcatctg cgcgctgctg
     6241 gatctgccgg ggcatctgaa ggatgatcgc gtgcttcgcc tgctgcgcga gacgatcgac
     6301 catttcggcc gcatcggcgg aatgctggtg ctgatccatc catccgccga tttaccgccg
     6361 gcgattgccg cgacgacgtt tgaagtatcg ttcccggaca aggtcgagct ggaaggcatc
     6421 ctgcgcgaga ccagccgaaa gctggcgtca gagcggcaga tcaccgtgaa cctatcgcgg
     6481 caggggttga atgcgatcgt ccgcaacttg cagggcctga gccgctcgca agcgcgacgg
     6541 gtgctgaccg atgcgatctg cgaagactgc cggctggacg agcacgatgt gaacgcggtg
     6601 ctggcgggca agcgcaaggc gctgagcggc gccggcatgc tgcaatacgt cgaatcgccg
     6661 gtcgatttga acgaagtcgg cggattggaa aagctgaagc agtgggtggt gcagcgccgc
     6721 ccggcgctgg agaacgaggc ggcgatcgaa ttcggtctca ccccgccgcg cggcgtgctg
     6781 atgctgggcg tgcagggcgc cggcaaaagc ctggccgcca aggccgtcgc caccgcgggg
     6841 caactgccgc tgctgcggat ggacgtgggc tcgctgtacg acccgtacat cggccaaagc
     6901 gaacgccggc tgcgcgatgc gctgcggcag gcggagttga tgtcgccggt ggtgctgtgg
     6961 atcgacgaga tcgaaaaagc gtttgcctcc gccgccagcg aaagcacgga tggcgggctg
     7021 tctcgccgga tgttcggcgc gctgctgacg tggatgcagg agcgaacagc gccagtcttc
     7081 ctgatcgcca ccgcgaacga catcgaagcc ctgccgccgg aactgctgcg caaggggcgg
     7141 ttcgacgaga tcttcttcgt cgatctgccg tcgctggaat cgcgcacgca gattgtggcg
     7201 attcacttgc gaaagcgcaa gcgcgacccg gcgaagtttg acatggccac gatcgccgac
     7261 gccgccgcgg ggtatagcgg agccgagatc gagcaggcga tcgtcggcgc gctgttcgac
     7321 gcgttcgacg caaagcgcga gctcaccacg gcggatgtgg tggcatcgct caagcgatcg
     7381 ccgccgttgt cggtaacgat ggcctagcgc gtcgccgcgt tgcgagaatg ggccatcgac
     7441 cgctgcgtgc cggcggattg aacccacgcc gggttcggag cgtactcgcg acgacccgga
     7501 tattctggcg cgcgaaaaac aatccgggtc gtcggagtac ctccgaaccc ggcgtgagac
     7561 atcacccact aatctcttgc catcgcttgc cagcgcccat ctgcctcctt acgattctcc
     7621 gctttcccca caacaagtac ctaacaagga ccgactgctc atggcgctca agattttggt
     7681 tgcggacaag cttgccgaag agggcctcga attcctcaag tccagcggca tcgatttcga
     7741 cgtgaaggtc gggttgagcg atgacgagct cgccgccgtc gcgggaaact acgatgcgat
     7801 gatcgcccgc tcggcgccga agaagtcggc catctccaag gcgctggcca atcccggtca
     7861 tctcaaagcc atcgcccgcg cgggcgtggg ggtcgataac atcgacctgg aagccgccac
     7921 ggccaagggc attctggttc tgaacacggc cgaggcatcg acgctgtcta ccgcggaaca
     7981 tgcgctggcg ctgatgctga gcctcgcccg taaaatcccg caggcgcacg cggcgctcaa
     8041 gtcgcagcag ctggattgga agaagcgaaa cacgtttcag ggcacgcagt tggccggcaa
     8101 gacgctcggc gtcgtcggcc tgggccgcat cggccgcacc gtcgccacgc gagcgctggc
     8161 gatggagatg aatgtcgtcg cgtacgaccc gttcttcagc gcccccaccg cccttgaagg
     8221 ccgcgtgaag ctgtacaagg atttcgacga attcctcagc aacatcgacg tcatcacctt
     8281 ccacgttccc ggcggcgagg cgaccaagca cctgctgaac cgcgaacgcc tgttcaacaa
     8341 agcccggccc aacctgctgg tcgtcaacga cgcccgaggt gaagtcgtgg atgaatacgc
     8401 gctggccgat gcgctgaagg aaaagaggat cgccggggcg gcgatcgacg tcttcgcggt
     8461 cgaaccgccg ccgaaagatc acccgctgtt cgggctggac aacgtcgtgc tgacgccgca
     8521 cctgggggcg agcaccgacg aagcgcagac cgccgtgtcg gtggacgccg ccaaggcgat
     8581 cgtcgcgtat ctgtaccgcg gggagattcg cggcgccgtc aacgccggtg gcattcgcct
     8641 cgacctgccg ccggacgaag cgccattcgc ggaactggcc cggcgcatcg ggcgattact
     8701 ggccggcgtc acggactccg gcatcaaaac gatcacgctt cgcgccagcg gcacgcgtgc
     8761 gccgcggttg atgaacacgc tgctgcgctt ggccgcggtg gaactgctca agccgcacct
     8821 gggtgaaggc agcgtaaacg tgatcaacgt cgatcacctc gcccgcggcc gcgggatcga
     8881 gatggtgcag gtccacgaac ccgccccgcc ttcgggcctg gtcggtgaca tactcggcgt
     8941 ccgcgtagac ggcccgaatg gcgaatcgca ccgcatcctc ggcaccgtct acgccgacgg
     9001 cctgccgcgc gtgctgcgcg tggacgatta cgcgatggac atgattcccg aaggctcgat
     9061 ggtcatcatc atcaacgaag accagcccgg cgtgatcggc accgtcggca ccgccttcgg
     9121 cgacgccaag gtgaacatcg ccgacatggt catcagccgc gcgttcaacg accagggcaa
     9181 ggcgacggcg ctgatggtga tcaagaccga cagcgaagcg ccggatgatc tgttgaacaa
     9241 actacgcgcc cgcccgggta tcgttcgcgc caagagcgtg aagctgccgc cgcgcggatg
     9301 atcgtgggaa tgcagaatag aagtaacgaa agagccgcgc acggagcgta ctcgcggagt
     9361 aagcggggtt atgggcgatc cgcttactgc gcgagtacgc t
//
LOCUS       DATIMH010000964         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3979373, whole genome shotgun sequence.
ACCESSION   DATIMH010000964 DATIMH010000000
VERSION     DATIMH010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3979373"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VLI90_16770"
     CDS             <1..527
                     /locus_tag="VLI90_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15917.1"
                     /translation="FEPTMAQPPAEERPMLPKGCVINVVCLPAIDESDELAAMMFAQL
                     LDIRGYCAKAVSVEKLASEMVEIVQQARADLVCVSALPPTASRHARYLCKRLHAALPG
                     LELVVGLWTARGDMKSAKQRIACAEPVRVVATLKDALAEVHQLVQPKLIERGENLPSP
                     PKSEDKPAIAGTRG"
     gene            1035..>3161
                     /locus_tag="VLI90_16775"
     CDS             1035..>3161
                     /locus_tag="VLI90_16775"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSV15918.1"
                     /translation="MTRAVAAGFERLESRQLFSAAALTIGGAPSPVDEGSSYSLNLSV
                     SNLPSGTNVTDYSITWGDGQVTPITGAAATNPLVVNHTYLDGPALNLNVTATATLSDA
                     EVLNSNTGSSTVTSDGSPDAAFGAGGTADTTLGLGAVTNNVAVQSNGQLVVAGVHGST
                     MSLVRLNSDGSVDGTFNLANPSLGVGTSSVARVLIETDPSDSSRTDYLVAGTVGSNGV
                     LWRIHADDGTPDASFGIGGKLTLGTTQTLDDATLDPNGNIVTLGMHGDTVTATRLLAD
                     GSLDATFAGGEQTYAYDFTGSAYGVVATSDSVVAVTNGFTWVTGDTLSHLLKFTNAGL
                     AGDAILQTNPSGPFSPSMHFDAIAMDANGNYVLGGRNLGGPSTASFAVARFDSNLNPD
                     SSFGSGGFVTTTAVGQGGGATGIAVGADGKIVAVGVRSAAGQSVLARYDNTGLLDPSF
                     GSGGIVLAGPSAGFVTPEAVVVQPDLKVLVPIIDASFTGPAGIVVRLGNSSNATSTPV
                     TVNVNNVAPTPSINVAPSSGSEGTAISLSGSVTDPSPVDTAAGLGLAWSVTKNGLAYG
                     GGGSGANFSFTPDDNGAYVVTLTATDKDGGSSSTDTPITVANVNPSTGAITSPAAAVR
                     GQNVSFASSFTDPGVLDSHTVTWDFGDSSPAVTSSVGAGVTGAVSASHAFTSAGTFNV
                     TLTVTDNDGGSGSSSKSFTVDIVDIQG"
BASE COUNT          555 a         1001 c         1046 g          559 t
ORIGIN      
        1 cgtttgaacc gacgatggcg caaccgccgg cggaggagcg gccgatgttg ccgaaggggt
       61 gcgtgatcaa cgtcgtctgc ctgccggcga tcgacgaatc cgacgagctg gcggccatga
      121 tgttcgcgca gttgttggac atccgcgggt attgtgcgaa ggccgtctcg gtcgagaagc
      181 tggccagcga gatggtggag atcgttcagc aggcgagggc ggacctggtg tgcgtctccg
      241 ccctgccgcc cacggcatcg cggcatgcgc gttatctgtg taagcgcctg cacgcggctt
      301 tgccggggct ggaactggtc gtcggcctgt ggaccgcccg tggcgacatg aagtcggcca
      361 agcagcgcat cgcctgcgcc gagccggtgc gcgtggtggc gacgttaaag gacgcgctgg
      421 cggaagtgca tcagttggtt caaccgaagt tgatcgaacg cggggagaat cttccgtcgc
      481 cgccgaagtc cgaggacaaa ccggcgattg cggggacgcg cgggtagaag catctgccgc
      541 ttgcggcttc gcaaactacg ccgcgaggat tgcgaagccg ccggcggcgg cgatcaactc
      601 accgacaccc aacgttaatc caggcaagat ttcccgcccc gccaccgtca tccaaaattg
      661 ctgcaggaac tggcaggcgg aaaccgatga tccggattgt ggatgccggt gggattccac
      721 cggtattttt tgttgcattg tcaaatttcg gcacgataat ccgaatccat ccggtgggtt
      781 ccaccggtca ggagagggaa ccaggtcgcg ggcaacgcct ggttccacaa gtgcatgtca
      841 aagttgactc actgtcgtcc ccatatctgg gtgactcttc tgcaattcat gctgcgcaat
      901 tgatctgaat cgcttggccg ttcagagccc tttgcgccac gcgtctacga atggaggctg
      961 tagtggctca tacatcgact cggtcgcggt ctggttctgt ctataacaat aagcgaatcc
     1021 aacgaaacgc ggcgatgacc cgcgctgtcg ccgcggggtt cgaacggctg gaaagccggc
     1081 agctgttcag cgccgcggcg ctgacgatcg gcggcgcacc gtcgccggtt gatgaaggat
     1141 ccagctactc gctgaacctg tcggtcagca atctcccgtc ggggacgaac gtcaccgact
     1201 actcgatcac ctggggcgac ggccaagtga cgccgatcac cggcgcggcg gcgacgaacc
     1261 cgctggtcgt caatcacacg tacctggacg gcccggcgct gaacctgaac gtgacggcga
     1321 cggcgacgct gagcgacgcc gaggtgttga actcaaatac gggttcgagc acggtcacca
     1381 gcgacggttc accggacgcc gcgttcggcg cgggtggcac ggccgacacc actctgggcc
     1441 tcggcgccgt caccaacaat gtggccgtgc aaagcaacgg tcagctggtc gtggccggtg
     1501 tgcacggcag cacgatgtcg ctggttcggt tgaactccga cggcagcgtc gacggcacgt
     1561 tcaacctggc gaacccgtcg ctgggcgtgg gcacgtcgtc ggttgcgcgg gtgctgatcg
     1621 aaaccgaccc gagcgactcg agccgaactg actacttggt tgccggcacg gttggctcga
     1681 acggtgtgct gtggcgcatt cacgccgatg acggcacgcc ggatgcttcg ttcggcatcg
     1741 gtggcaagct gacgctgggg acgacgcaga cgctggacga tgcgacgctg gaccccaacg
     1801 gtaacatcgt cacgctaggc atgcatggcg acaccgtcac cgcgacgcgc ctgctggccg
     1861 acgggtcgct cgatgcgacc ttcgcgggtg gcgagcagac ctatgcctat gacttcaccg
     1921 gctcggcgta cggcgtggtt gcgacgagcg actctgtggt cgctgtgacc aacggcttca
     1981 cgtgggtcac tggcgacacg ctgagccacc tgctgaagtt caccaacgcc ggtctggccg
     2041 gcgacgcgat cctgcagacc aaccccagcg gaccattcag cccgtcgatg cacttcgatg
     2101 cgatcgcgat ggacgccaac ggcaactacg tgctgggcgg tcgaaacctt ggtggtccgt
     2161 ccaccgcatc gttcgccgtc gctcgcttcg actccaacct caaccccgat tccagcttcg
     2221 gctccggcgg tttcgtgacg acgaccgcgg tgggccaggg cggtggcgcg accggcattg
     2281 cggttggcgc tgatggcaag atcgtcgccg tcggggtgcg cagcgccgcg ggccagagcg
     2341 tgcttgcccg ttatgacaac accggtttgc tcgaccccag tttcggcagc ggcggcatcg
     2401 tgctggccgg tcccagcgcc ggctttgtta ccccggaggc cgtggtcgtt cagccggatc
     2461 tgaaggtgct cgtgccgatc attgacgctt ccttcaccgg cccggcaggc atcgtcgtgc
     2521 gtctcggcaa cagcagcaac gcgaccagca cgccggtcac ggtgaatgtg aacaatgtgg
     2581 ccccgacgcc gtcgatcaac gtcgctccat ccagcggcag cgaaggcacg gcgatcagct
     2641 tgagcggatc tgtgactgat ccgtcgccgg tggacaccgc cgcggggctg ggcctcgcct
     2701 ggtcggtcac caagaacggc ttggcgtatg gcggcggtgg ttcgggcgcc aacttctcgt
     2761 tcacgccgga cgacaacggc gcgtatgtcg tcacactgac cgccaccgat aaggacggcg
     2821 ggagcagctc gaccgatacg ccgatcaccg ttgccaacgt gaacccctcc accggcgcga
     2881 tcacctcgcc ggcggccgcg gtgcgtgggc agaacgtgtc gttcgccagc agcttcaccg
     2941 atccgggcgt gctcgattcg cacacggtga cgtgggactt cggcgacagt tcccccgccg
     3001 ttaccagcag cgtcggcgcg ggcgtcaccg gcgcggttag cgccagccac gcgttcacct
     3061 cggctggaac gttcaacgta acgttgaccg tgaccgacaa cgacggcggc agcgggtcgt
     3121 ccagcaagag tttcacggtc gacatcgtcg atattcaggg c
//
LOCUS       DATIMH010000965         4396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1389462, whole genome shotgun sequence.
ACCESSION   DATIMH010000965 DATIMH010000000
VERSION     DATIMH010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389462"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VLI90_16780"
     CDS             <1..315
                     /locus_tag="VLI90_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15919.1"
                     /translation="IYDFGPHVSADGTIEYLSDSVGGKLKHMLIVCRYNAGSDLIAVK
                     LNALGDGVDYALTGIVGFTNFNAPLDLCEDPTNGNIYVSEYGGKCITLLRPIAAVSAS
                     LK"
     gene            complement(807..1256)
                     /locus_tag="VLI90_16785"
     CDS             complement(807..1256)
                     /locus_tag="VLI90_16785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSV15920.1"
                     /translation="MIFTSTSEYAIRGMTELAVREPEGRMLLDDLVAGTSLPRDFMAK
                     IFQRLAKAGLLDSAKGRGGGFSLARPPHKITLEDIVEAIEGPEAYDHCIVGLERCNDQ
                     MRCPQHDLYKPIRQRWHDYLKTTTLADLASSLKSKMAKKAGRSSAKG"
     gene            1477..4215
                     /locus_tag="VLI90_16790"
     CDS             1477..4215
                     /locus_tag="VLI90_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15921.1"
                     /translation="MLLIIPSIRAAGAAGDNAPLQTFTSDDVSAVIGAAQMSADGSRI
                     VIDEYKVGDAGVPRLSAARVFDVATRKLLLEARPAAGDQAVEIKMSPAAGRFGLISRL
                     HGDGSTSDELVEVETGRKLYASSARYGGGGGFFSPDRRQAFVCIQPPNGEDSFDAATF
                     AVVALPSGKETGRLRLEHAGPSAVSPDGRYVVCNDLIYQLPGGQKVCQLAPTKGLQYF
                     AAQAFTGDGQRYRVANREGTIFTWDVNSGRVISRIEPQVTMRLDGQAAFSKDAAQLLV
                     AESGAIVFYDAETVREVGRWSHGPERRSRAILASLSDRAIAIAVEQRDVHQIAVVRLP
                     DAHDLQIAPAPAPPRPAAPAATAPAAALPPHAPADLASADWWFDQAEREISALPANQQ
                     PPAIVSLLQARLQSGGDDEAAAARLLQKLASLAPPMERQRWEQELAKNRIRRLGSGDA
                     GKAVALAEPFGPVSWQTQNVWTEVAAAQARAGEIGAAILTVNQLGKDAGGRAAAAIID
                     AELTAGKVAEARRFSDSIANDFWRQACLGKIATKLAKSGDVDGCLAIMRTLWAQSVVS
                     DVQITTVQAAAGASNDAGANRIIDSLPEADREHARIFLLKSQLARGDMPSALVTRGTI
                     SETQQMIFHVESEFTQARVRQGLFDDAVAGIGTSNEPGEVAWATCKALIAAGKLDVAD
                     QMIAKFSTPRRWRASDADSLRASLKCNVAIAMANADRLPDARRLLTQADALDAKPAVG
                     RSNEISLALAAAYRRAGDHSHFDELRKPYVNAVTAVTGAPSRAESLGNLLSFDLATGN
                     IAAVQASLESADDALRLGRTLQVLVTLSGADDGSSRPPPLDQVRKLLSTLIDARAERA
                     VPVALTAAAARHARDGKLADWLAWTATLSPIYRIAADQATGSAVLDVNAAPEHLVR"
BASE COUNT          832 a         1289 c         1433 g          842 t
ORIGIN      
        1 atttacgact tcggcccgca cgtgtccgcc gacggcacga tcgaatatct cagcgacagc
       61 gtcggcggaa agctgaagca catgctgatc gtctgccggt acaacgcggg atcggatctg
      121 atcgcggtga agctgaacgc attgggcgat ggcgtcgatt acgcgctgac gggcattgtc
      181 ggttttacaa acttcaatgc gccgctcgat ctgtgcgagg atcccaccaa cggcaacatc
      241 tacgtcagcg aatacggcgg aaagtgcatc acgttgctgc ggccgatcgc ggcggtgtcg
      301 gcctccttga aatgagtttt gcgcacgtct cgaaaagcag tgcaaacgcg aagtcgcgaa
      361 ctgggcgaag gctgacgcga agaagacacg gcaggtactg aagttcaggt cacggcttgg
      421 gaatcattaa tgatgacgcg ctaggtccgc ccgcgatcgc acctatctgt tcgtcccgcc
      481 atccaatggc aaagaagccc gaggcgattt acaggtaccg ccgattcatc caatgatcgg
      541 gtggcgggat ttcaattagt cacttgaatg aggcgcaata tatttgctta attgttgccg
      601 cccaactgtc gtaacgcatg caggtgcaga cagatggaga cggcaatatc tcagagatgc
      661 ccggaaacaa gcgcctcatt ctgtctcacg gcgatcgctt ggattgaagg aagggggcgt
      721 tagttcagtg acgatcgctg gcaaaaaaca aatcgaagcg tcctttactt gtcgccgctg
      781 aaggggtgtc gcaatggccg tacggttcaa cccttcgccg aagaacgccc cgccttcttc
      841 gccatcttcg actttagtga agacgccaga tccgccagcg tggtcgtctt cagataatca
      901 tgccagcgct ggcggatggg tttgtaaaga tcgtgctgcg ggcagcgcat ctggtcgttg
      961 cagcgttcga ggccgacgat gcagtgatcg tacgcttctg gcccttcgat cgcctcgacg
     1021 atgtcctcca gcgtgatctt atgcggcggc cgagccaggg aaaacccacc cccgcggcct
     1081 ttggcgctgt ccaatagacc agcctttgcc agtcgttgga agattttcgc catgaaatcg
     1141 cgcggcaacg acgtgccggc gaccaaatca tcgagcagca tgcggccctc gggctcgcgc
     1201 acggccaact cggtcattcc acggatcgca tattcgctgg tgctggtgaa gatcatctca
     1261 ggacgatctt atccgctatt acggcgggag tccaagcgac gctggaagcg cggccattga
     1321 ctgtggagcc tcgctcgctc cgaagccggc acggagatat tctggcggcg cgtcgggctt
     1381 cactctccaa atgggcgtgc ggaaatgagc aaaacttttg gatcgtgcgg cggcggcacg
     1441 cgttggcttc gctgtcgatt gtgcgtggcg cttgtcgtgc ttctgatcat tccgtcgatt
     1501 cgcgcggcgg gcgcggctgg cgataacgct ccacttcaaa ccttcaccag cgacgacgtc
     1561 agcgccgtga tcggggcggc gcagatgtcc gccgatggct cacgcattgt catcgacgag
     1621 tacaaggtgg gtgatgcggg cgtgccgcgc ttgtcggctg cgcgcgtctt cgatgtggcg
     1681 acgcggaagt tgctgcttga ggcgagacct gccgccgggg atcaggcggt tgaaataaaa
     1741 atgtcgccgg ccgccggccg ctttggactc atcagcaggc tgcatgggga tgggtctacc
     1801 agcgatgaac tcgtcgaggt cgagacgggt cgaaagctgt acgcgtcgtc ggcccgatac
     1861 ggcggtggcg gcggcttttt ttcgcctgat cggcggcagg cctttgtgtg catccagcct
     1921 ccgaacggtg aggacagctt cgacgccgcg acgttcgcgg ttgtcgcctt gccatcgggc
     1981 aaggagaccg ggcgattgcg actggagcac gccggcccgt ccgccgtctc gccggatgga
     2041 aggtacgtcg tctgcaacga tctcatctat caacttcccg gcggtcaaaa ggtatgccag
     2101 ttggcgccga ccaaggggct gcaatacttc gccgcccaag cgttcaccgg cgacgggcag
     2161 cgctatcgcg tggccaatcg tgagggaacg atcttcacct gggacgtgaa cagcggccgg
     2221 gtgatctcac gcatcgagcc gcaggtcacg atgcgattgg acgggcaggc ggcgttttcg
     2281 aaagacgcgg ctcaattgct tgttgccgaa tccggagcca tcgttttcta tgatgcggag
     2341 acagtgcgtg aagtgggtcg gtggtcgcat gggccggagc gacgctctcg agcgattttg
     2401 gcttcgctgt cggatcgcgc gatcgccatt gcggtcgagc agagagatgt tcatcagatt
     2461 gcggtagtgc ggctgccgga tgcgcacgat ctgcaaatcg cgcccgcgcc ggcgccaccg
     2521 cggccggcag cgcccgcggc gacggcgcct gccgcggcgc tcccaccgca tgcgccggcc
     2581 gatttggcta gcgcggactg gtggttcgat caggcggaac gtgagatttc cgcacttcca
     2641 gcgaatcagc agcctccggc aatcgtgagc ctgctgcagg cgcgcctcca aagcggtggt
     2701 gacgacgaag cagcggcggc acggttgctg caaaagctgg catcgcttgc gccacccatg
     2761 gaacgccagc gatgggagca ggagctggcg aagaatcgca tccgtcgctt gggatctggc
     2821 gatgccggca aggcggtggc gcttgccgag cccttcggcc cggtctcgtg gcaaacgcaa
     2881 aatgtatgga ccgaagtggc tgcggctcag gcccgcgccg gggagatcgg cgcggccatt
     2941 ttaacggtta atcaattggg caaggacgcc ggtgggcgag cggcggccgc aatcatcgac
     3001 gcggaattga ccgcgggaaa ggtcgccgaa gcgcggcggt tcagtgacag catcgcgaac
     3061 gatttttggc ggcaggcctg tctcggcaag atcgcgacca aactggcgaa aagtggagac
     3121 gtcgacggtt gcctcgccat catgcgcacg ctctgggcac aatctgtggt ttcggatgtt
     3181 cagatcacca ccgtccaagc ggcggccggg gccagcaatg atgcgggggc gaatcgcatc
     3241 atcgactcac ttccggaggc ggaccgcgag catgcccgaa tctttctgct gaagtcgcag
     3301 ttggcgcgcg gcgatatgcc atcagcgctg gtcacgcgcg gcacgatcag cgaaacgcag
     3361 cagatgatct ttcacgtcga gtcggagttt acccaagcgc gcgtccggca ggggctgttc
     3421 gacgatgccg tcgcgggcat cggtacatcc aatgaacccg gcgaggtcgc gtgggcgacg
     3481 tgcaaggcac tgattgccgc cggcaagttg gatgtggccg atcagatgat cgctaaattc
     3541 tccacgcctc gccgctggcg agcatcagat gcagactcgc ttcgcgcgtc gctcaaatgc
     3601 aatgttgcta tcgcgatggc caacgcggat cgcctgcccg atgcgcgacg tttgctgaca
     3661 caggccgatg cgctggatgc caagcccgcc gtcgggcgat ccaatgagat ttccctcgcg
     3721 ctcgccgccg cgtatcggcg cgccggcgac cattcgcact tcgacgagct tcgcaagcca
     3781 tacgtcaatg cggtgacggc ggtaacgggt gcgccttcga gagcggagtc gctgggcaac
     3841 ctcctcagct tcgacctggc caccggcaac atcgccgcgg tgcaggcgtc gctggaatcg
     3901 gctgacgatg cactgcgact tggccggacg cttcaggtgc tcgtcacatt gagcggtgca
     3961 gatgacggca gctccagacc accgccgttg gatcaagtgc gaaagctgct aagcacgtta
     4021 atcgatgccc gggcagaacg ggctgttccg gtggcactga ccgccgccgc cgcgcggcat
     4081 gctcgtgacg gaaagctcgc ggactggctc gcgtggacgg ccacgctctc gccgatctat
     4141 cgcatcgccg cggaccaagc gacaggatcg gcggtgcttg acgtaaatgc agcgccggaa
     4201 cacctcgtgc gatgaagcac gccgatgcgg tgcgccgctc aggagcggct tgcgttcctt
     4261 tgtagcggtc atgctgagcg gagcacgtac tcgtgcgcag tcgaggatat tgctaggaag
     4321 tttgtgaatg accaatcgcg cgagtcgcac gggagtcgct tgcagtttcg cagaccagta
     4381 actgggtctt gtccgt
//
LOCUS       DATIMH010000966         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_520848, whole genome shotgun sequence.
ACCESSION   DATIMH010000966 DATIMH010000000
VERSION     DATIMH010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520848"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VLI90_16795"
     CDS             <1..804
                     /locus_tag="VLI90_16795"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSV15922.1"
                     /translation="IRHLLTHTAGIRAAASNFSRDPWDVTIARICNAKIEPSWTPGKK
                     AGYHVASSWFILGELVHRRDERRRPFEQYVREEIFLPLRMTDSWIGIPADRYAAYGNR
                     IGIMQNTDGGESPTPHTWDTPEMAALCKPGGNGRGPMRELARFYQMLLNGGELDGARI
                     ISAQTVEAMTARQRVGMFDHTFKHLMDWGLGFIPNNNQYGVDTIRYGYSPHASWRTFG
                     HSGHQSSVAFMDVKRQIVAALVFNGTPGEEAHDQRVRSVLTGMYEDLSA"
     gene            complement(821..>2324)
                     /locus_tag="VLI90_16800"
     CDS             complement(821..>2324)
                     /locus_tag="VLI90_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15923.1"
                     /translation="PCYGRGCNCTAGSPSILYESLWSGARMSTTAETQHRESTHRWFR
                     WAFLGAALLLFLILLAHVLWGSYAERRLEAQVARLRDEGEPMTLEQLALPVVPDAANA
                     SLDYQAAGKSIDTSAPAFHKWSSAERGHFQFPLTDDERATFNAIVGANAAAMKLVMAA
                     RDKQAGQWGDKFTKPYFTSTLDFTSSRNLAELLNAAAYTAHAAGRDHDAVEHVHDLLR
                     LANASMNRPTLIAHLVAVGISALGADTASAIAVDLHAPRPQVEALLRQLQEERAIFDS
                     RRLALRSERIFELEACYNFVDGVLSPNAIATPPNTGQRLVGYAIKPLAMDDGRQAMIY
                     VQDVLKATEAPDWPTAQRQMPTAFMKEVEASPIRHMMARLTMPALDRAALSSYLAIAK
                     RRLAATALAVRLYALDHDGQLPRTLEALVPRYLPAVPIDPLSGQAIRYINEAKNPRIY
                     SVGRDGVDDGGKPEAQSLGTGRYGWKGDDVVNLTRQPREVEEPDGATTQP"
BASE COUNT          425 a          812 c          696 g          391 t
ORIGIN      
        1 atccgccacc tgctgaccca caccgccggc attcgcgccg ccgccagcaa cttcagccgc
       61 gatccgtggg acgtgacgat cgcccgcatc tgcaacgcca agatcgaacc cagctggacg
      121 ccaggcaaaa aggcgggata tcacgtcgca tcgagctggt tcatcctcgg cgagttggtc
      181 caccgccgcg atgaacgccg ccggccgttc gagcagtacg tgcgcgaaga gatcttcctg
      241 ccgctgcgga tgaccgacag ctggatcggc atcccggcgg atcgttacgc ggcgtacggc
      301 aaccgcatcg gcatcatgca aaacaccgac ggcggcgaat cacccacacc gcacacctgg
      361 gacacgcccg agatggccgc gttgtgcaag cccggtggca acggccgcgg acccatgcgc
      421 gagttagcgc gattttatca gatgctgcta aatggcgggg agctggacgg cgcgcggatc
      481 atctcagccc agacggtcga agcgatgacc gcacgccagc gcgtcggcat gttcgaccac
      541 acgttcaagc acctgatgga ctggggcctg ggcttcattc ccaacaacaa ccagtacggc
      601 gtggacacga tccgctacgg ctacagcccg cacgccagct ggcgcacgtt cggccacagc
      661 gggcaccagt cgtccgtcgc gttcatggac gtcaaacgcc aaatcgtcgc cgccctcgtc
      721 ttcaacggca cccccggcga agaagcgcac gaccaacgcg tgagaagcgt gctgacgggg
      781 atgtatgaag acctgagcgc ttagtcgaac tccccacgac tcacggctgt gtcgtcgcgc
      841 catccggttc ctccacctcc cgcggttggc gcgtcaggtt caccacgtca tcgcccttcc
      901 acccgtacct gcccgtgcca agtgattgcg cctccggctt gccgccgtca tcgacgccgt
      961 ctcggccgac gctgtagatc ctcggattct tcgcttcgtt gatatatcga atcgcctgcc
     1021 ccgacagtgg atcgatcggc accgccggca gatagcgcgg caccaacgcc tcgagcgtgc
     1081 gcggcaactg gccatcgtga tccagcgcgt aaagtcgcac cgccagcgcg gtcgccgcca
     1141 ggcggcgctt ggcgatggcc agataactcg acagcgccgc gcgatcgagg gcgggcatcg
     1201 tcagccgcgc catcatgtga cggatcgggc tcgcctccac ttccttcatg aacgccgtgg
     1261 gcatctgccg ctgcgctgtt ggccagtcgg gcgcttcggt ggccttcagc acatcctgca
     1321 cgtaaatcat cgcctgccgc ccgtcgtcca tcgccagcgg tttgatggca tagcccacca
     1381 gccgctggcc cgtgttgggt ggcgtggcaa tcgcgttggg agacagcacg ccatcaacga
     1441 agttgtagca cgcctcgagt tcgaagatcc gctccgatcg caacgccagc cgccgactat
     1501 cgaagatggc ccgctcctcc tgcagttgcc gcagtagcgc ttcaacctgc ggccgcggcg
     1561 cgtgcagatc gaccgcaatc gccgatgccg tgtccgcccc cagcgcagaa atccccaccg
     1621 ccaccaggtg cgcgatcaac gtcggccgat tcatcgacgc attcgccaat cgaagcagat
     1681 catgaacgtg ctcgaccgcg tcatgatccc gccccgccgc atgggcggtg tacgcggcgg
     1741 cgttcagcaa ctccgccagg ttccgcgacg acgtgaagtc caaggtggac gtgaagtacg
     1801 gcttggtgaa tttgtcgccc cattggccgg cttgcttgtc acgcgcggcc atcacgagct
     1861 tcatcgccgc agcgttcgcg ccgacgatcg cgttgaacgt tgcccgttcg tcatccgtca
     1921 gtggaaattg gaaatgaccg cgttccgccg atgaccattt gtgaaacgct ggcgctgacg
     1981 tatcgatcga cttccccgcc gcctgatagt ccagcgacgc attggccgcg tccggcacga
     2041 ccggcagcgc cagctgctcc agcgtcatcg gctcgccttc atcgcgcagg cgcgcgacct
     2101 gcgcctccaa ccgccgctcg gcgtacgagc cccacagcac gtgcgccagc aggattaaga
     2161 agagcagcag cgcagcgccc agaaacgccc agcggaacca gcggtgggtg gattcgcggt
     2221 gctgtgtctc ggcagtggtg ctcatgcgtg cccctgacca gagggattca tacagaattg
     2281 acggtgagcc ggcggtgcaa ttgcaccctc ttccgtagca cggg
//
LOCUS       DATIMH010000967         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_810523, whole genome shotgun sequence.
ACCESSION   DATIMH010000967 DATIMH010000000
VERSION     DATIMH010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810523"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..113
                     /locus_tag="VLI90_16805"
     tRNA            43..113
                     /locus_tag="VLI90_16805"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Gln,seq:ctg)
     gene            complement(174..632)
                     /locus_tag="VLI90_16810"
     CDS             complement(174..632)
                     /locus_tag="VLI90_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15924.1"
                     /translation="MLILPIIGGCASSAAKDKARADRVAAAQRSLDATVQKLNLTPAQ
                     QSKLATVYKWQSQQSASNAHLAVGDERLFLDNGQFSQRLATVEDVDRSVLGKGMPTDP
                     NGIQSIVDVHVRSILSSEQYAMYRNDLSPEERAALADQLRNGMRSTAGQR"
     gene            774..1847
                     /locus_tag="VLI90_16815"
     CDS             774..1847
                     /locus_tag="VLI90_16815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV15925.1"
                     /translation="MERRAFGDTNLTVSAISLGCWIFGVDWWGHYTDERAMELCRFSY
                     DHGITFFDNGDAYGNGRAETLFGKFLKESGIARDKIEIGSKFGYDFYSDPGEAGSHRE
                     RKQDFSPGFMRSSLEKSLARLGTDYVDLYMAHNIKLPQFRDDLFAELEKLKDEGKIKV
                     WGVSLGPAIGWREEGFKAIIDHNAKAVQTVFNLFEQSPGREFCETAHAYKAGVLARVH
                     DNSSILKDVVKIDTMIDKNDHRQFRDQAWKIYGLKKLELVRHYASDHGMTVHQLACRW
                     LLQQPALTSITGTFLNEHEIREAAESVGKPKLSMQELRQIAEDYAKDFGLGAEAHPCD
                     LKSSTDPSGRVRSGYVPSPVLIS"
     gene            complement(1921..>2477)
                     /gene="surE"
                     /locus_tag="VLI90_16820"
     CDS             complement(1921..>2477)
                     /gene="surE"
                     /locus_tag="VLI90_16820"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HSV15926.1"
                     /translation="PADCVKLAINQLLPRQPDLVVSGLNAGANVGINVIYSGTVAAAI
                     EAAFLGCRAVATSLFLRKEVEADFAGAARMSRETIELLLKRGLSGGQVASVNIPALPA
                     GEAAAGIRIVRQCTRPWVDTYERRTDPRGRDYFWNNSVFTLGDTEDDTDVAALRDRYV
                     TVTPLQFDLTDHGVLKRWGTSDER"
BASE COUNT          450 a          734 c          808 g          485 t
ORIGIN      
        1 gcaatttcgc gggtaaactt tagggcggtt agataacccg tttggggaat ggtgtaacgg
       61 tagcacaagc gcctctggag cgctttgtct aggttcgaat cctagttccc cagttcttca
      121 gcccgcgcgt cctgatcggt cgggcgggct tttttgtttt tcggggatcg atctcaccgc
      181 tgcccggcgg tgcttcgcat gccgtttcgc agttggtcgg ccaacgcggc ccgctcttcg
      241 ggcgacagat catttcgata catcgcgtat tgctcggacg aaaggatcga gcggacgtgc
      301 acgtcgacga tcgattggat cccgttggga tcggtgggca tgcccttgcc aaggacggag
      361 cgatcgacgt cttcgacggt ggcaagtcgc tggctgaact ggccattgtc gaggaagagg
      421 cgttcatcgc caacggcgag gtgggcgttg gaggcggatt gctggctctg ccatttgtag
      481 accgtcgcca gcttcgattg ttgcgcgggc gtcaggttca gcttctgcac cgttgcgtcg
      541 aggctgcgct gggcggcggc gactcggtcg gcccgggctt tgtccttcgc ggcggagctg
      601 gcgcagccgc cgatgatggg aaggatcaac attgagaaaa tcgcggccgc gtggggtgcc
      661 cggttggtca tggcgggaat tgtaacaagt tgccgccgcc cgcggccacg ggcggcggct
      721 ttacatttgt tactgccctt gcagtgctac gcgcggcgtc tatcatcgcc gggatggaac
      781 gtcgcgcttt tggtgacacg aatttgaccg tctcggccat ctcgcttggg tgctggatct
      841 tcggtgtgga ctggtgggga cactacaccg acgaacgcgc gatggagctg tgccgattct
      901 cctacgatca cggcatcacg ttcttcgaca acggcgacgc ctacggcaac gggcgcgccg
      961 aaacgctgtt cgggaagttc ttgaaagagt cgggcatcgc gcgcgacaaa atcgagatcg
     1021 gcagcaagtt cggctacgac ttttactccg accccggcga agccggcagc catcgcgagc
     1081 gcaagcagga tttttcgccg ggcttcatgc gctcgtcgct ggaaaaatcg ctggcgcggc
     1141 tgggcacaga ttacgtggac ctgtatatgg cccacaacat caagctgccg cagtttcgcg
     1201 atgatctgtt cgcggaactc gagaagctga aggacgaggg gaagatcaag gtctggggcg
     1261 tgagcctggg cccagcgatc ggctggcgcg aagagggctt caaggcgatc atcgaccaca
     1321 acgccaaggc cgtgcagaca gtcttcaacc tgttcgagca aagccccggc cgggaatttt
     1381 gcgagacggc ccacgcgtac aaagccggcg tgctggcgcg cgtgcatgac aacagctcga
     1441 tcctgaagga cgtcgtgaag atcgacacaa tgatcgataa gaacgaccac cgccaattcc
     1501 gcgatcaggc gtggaagatc tacggcttga aaaagctgga gctggttcgc cactacgcca
     1561 gcgatcatgg catgaccgtg caccagctgg cgtgccgctg gctgttgcag cagcctgcgc
     1621 tgacgagcat cactggcacg ttcctcaacg aacacgagat ccgcgaggcg gcggagtcgg
     1681 tgggcaagcc aaagctgtcg atgcaggagc tgcggcagat cgcggaggat tatgcgaagg
     1741 atttcggatt gggggcggag gcacatccgt gtgatttgaa gagcagcacg gatcccagtg
     1801 ggcgggtgag aagcggctac gttccgtcgc cggtgttgat tagctaggtg cgcgccagcc
     1861 gcttgcggct tcgcaaacca cgtcgcttgg tctgcgaagc cgcaagcggc tgatcgcgat
     1921 tcatcgctcg tccgatgttc cccaccgctt caataccccg tgatccgtca gatcaaactg
     1981 caacggcgtc acggtgacgt accggtcgcg cagcgctgcg acgtccgtgt cgtcttcggt
     2041 gtcccccagc gtgaacacgc tgttgttcca gaaataatcc cgcccgcgcg gatcggtgcg
     2101 gcgttcgtac gtgtctaccc acggccgggt gcactggcgg acgatgcgga tgccggccgc
     2161 cgcttcgccg gccggaagcg cggggatgtt gacgctcgcg acctgcccgc cgctcaggcc
     2221 tcgcttcaac aatagctcga tggtctcgcg gctcatccgc gccgctcccg cgaagtcagc
     2281 ttccacttcc ttgcgcagga acagcgacgt cgcgaccgcg cggcaaccca gaaacgcggc
     2341 ctcgatggcg gcggcgaccg tgccgctgta gatcacgtta atgcccacat tcgccccggc
     2401 gttcaatccg ctgacgacca gatccggctg ccgcggcagc agctggttga tcgccagctt
     2461 cacacagtcc gctgggc
//
LOCUS       DATIMH010000968         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_521721, whole genome shotgun sequence.
ACCESSION   DATIMH010000968 DATIMH010000000
VERSION     DATIMH010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521721"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VLI90_16825"
     CDS             complement(<1..731)
                     /locus_tag="VLI90_16825"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSV15927.1"
                     /translation="MSVAGKYRMVAIDLDGTLLSPFGEVTPRAKAAIHRCLSAGLLVC
                     FATGRNWTESRAVLDAVAHYDTAVFVGGAMVVDTKHRMTLHRTMMDPALAGSICGHFE
                     RTGQAALALQDTGAAGVDYLVSADVPVNDATAQWMSITAARVHRVPTLSDPAAHEHTI
                     RVGIVAPPEQTQAAKRELEERHGSAIMVHSLFVPAYGVEVLEVFDPSVNKWAGLLHVA
                     RHHGIEPEQIIAIGDDLNDLAMIENA"
     gene            complement(853..>1641)
                     /locus_tag="VLI90_16830"
     CDS             complement(853..>1641)
                     /locus_tag="VLI90_16830"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSV15928.1"
                     /translation="AYRAAAGTSLVSPGNDPTLRILMDHATRHKVAVEPISFSSRDVA
                     ADVARVAREREADLVLIGFHKPVFGKTILGGVVHRVLTSVDADVGVFVDRGFLGARRV
                     LVPFTGDSHDRLAIRLAHRLLKNVNAEVTVLHVVPPKGADAGDALEAVRREFQGAGKE
                     PSPSIRFDIVQDASPVDAVLREAQKGYDLVLIGVSEEWGLESHLFGFRPERIAEKVGC
                     SLLIVRRHKQMIATLAAPALPAQEPATHTPAPPPPKPIASQSSA"
BASE COUNT          283 a          577 c          495 g          286 t
ORIGIN      
        1 gcgttctcga tcatcgccag gtcattcagg tcgtcaccga tcgcgatgat ctgctccggt
       61 tcgatcccat ggtgccttgc gacatgaagc aacccggccc acttgttcac cgacgggtcg
      121 aagacttcca gcacctcgac gccgtatgcc ggcacgaaaa gactgtgcac catgatcgcg
      181 ctgccgtgcc gctcttccaa ctcgcgcttc gccgcctgcg tctgctccgg cggggcgacg
      241 atgccgacgc ggatcgtgtg ctcgtgcgcc gccggatcgg aaagcgtcgg cacgcggtgg
      301 actcgggcgg ccgtgatgct catccactgc gcggtcgcgt cgttgaccgg tacatccgcg
      361 ctgaccagat aatccacgcc ggctgcgccg gtatcctgca gcgccagcgc tgcttgtccg
      421 gtgcgctcga aatgcccgca gatgctgccc gccaaagcag ggtccatcat cgtgcggtgg
      481 agcgtcatgc ggtgcttcgt atccaccacc atcgcgccgc cgacgaacac ggcggtgtca
      541 tagtgggcga cggcgtcgag cactgcgcgg ctctcggtcc agttccgccc cgttgcaaaa
      601 cagacgagca gccccgccga caggcaccga tgaatcgccg cctttgcccg cggcgtgacc
      661 tccccaaacg gcgacaacaa cgtcccgtcc aggtcgatcg cgaccatccg atacttgccg
      721 gccacactca cgtccgcttc gccggggtct tcaacccgtt tgatgggcgg gagcgtactg
      781 tcttccgacc gctttgcaac tactaaatga ttttttgacg acttatccac aacgtgtagt
      841 ggtgagcgtg attcaagcac tggattgcga tgcgatgggc ttcggcggcg gcggagccgg
      901 ggtgtgagtc gccggctcct gcgcgggcaa cgccggcgcc gcaagtgttg caatcatctg
      961 cttgtgccta cggacgatca acagcgagca acccactttt tccgcgatcc gttcggggcg
     1021 gaagccaaac aggtgcgact ccagtcccca ttcctccgac acgccaatca agaccaggtc
     1081 ataacctttc tgcgcctcgc gcagcaccgc atcgaccggc gacgcatcct gcacgatatc
     1141 aaatcggatc gagggcgacg gctccttccc cgccccctgg aactcgcgcc gcaccgcctc
     1201 gagcgcgtcc ccggcatcgg cgcctttcgg tggcacgacg tgcagcacgg tcacctcggc
     1261 attgacgttc ttcaacaggc gatgggccag gcgaatcgcg aggcgatcgt gcgagtcccc
     1321 ggtgaatggg accagcacgc gccgcgcgcc gaggaacccg cgatcgacga acacgccgac
     1381 gtctgcgtcc acgctggtga gcacgcgatg caccacgccg ccgaggatcg tcttgccgaa
     1441 gaccggcttg tgaaacccga tcagcaccag gtccgcttcc cgctcccgcg cgacccgcgc
     1501 cacatcggcc gcgacatccc ggctgctgaa ggagatcggc tcgacggcaa ccttgtgacg
     1561 cgtcgcgtgg tccatcagaa tccgcagcgt tgggtcgttt cccggcgaca cgagcgacgt
     1621 gcccgcggcg gcccgatagg c
//
LOCUS       DATIMH010000969         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2252115, whole genome shotgun sequence.
ACCESSION   DATIMH010000969 DATIMH010000000
VERSION     DATIMH010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2252115"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..899
                     /locus_tag="VLI90_16835"
     CDS             273..899
                     /locus_tag="VLI90_16835"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HSV15929.1"
                     /translation="MSTDAQTQSLKGREIIVAVCGGIAAYKVADVVSKLVQRGAGVSV
                     VMTDDAQRFVAPLTFQALSGRVVRTSTFELPDSSDPQHISLSERADLMLVAPATANII
                     AKAAHGVCDELVSLMICAAACPVVFAPAMNDRMWANPIAQENVAKLKLHGYRFIGPEA
                     GWLACRNVGAGRLAEPATIVDDVAAILAHSALPPATSDDRTIHSRSSP"
     gene            921..1376
                     /locus_tag="VLI90_16840"
     CDS             921..1376
                     /locus_tag="VLI90_16840"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSV15930.1"
                     /translation="MAEKQIDRKRLIELLNEDLSREYQAVIAYVVYSKVIKGAAYMNI
                     AAELEKHAGEELEHALKIADQIDYLGGMPVTKPQPVKTSEKAEDMLRFDLENERVTIA
                     NYRTRVRQADALGEFALSETLREILVQEQEHLTDLAAALGIDAPDPGEA"
     gene            1609..2238
                     /locus_tag="VLI90_16845"
     CDS             1609..2238
                     /locus_tag="VLI90_16845"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HSV15931.1"
                     /translation="MRVLITAGPTREPIDPVRYITNRSSGYMGKALADAAIKAGHSTT
                     LIFGPVNITTPTEARRIDVDTSRQMYDAVMAEFPNHDLLIMAAAVADYRPRRIRNEKL
                     ERGGTLVVEFEATEDILAEAGKIKRLDQRTVGFSLVSRGDLARSMEKLRRKNLDLIVY
                     NTFETQGSKSIESVLLWPSGRSEELPSRSKADLADILLKRAAELFASTD"
     gene            2532..>2854
                     /gene="dut"
                     /locus_tag="VLI90_16850"
     CDS             2532..>2854
                     /gene="dut"
                     /locus_tag="VLI90_16850"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HSV15932.1"
                     /translation="MPTKMTEHAAGFDLAAAVASPVVLQPGEIQLIPCGFAMAVPHGY
                     EAQVRPRSGLSSKHGVTMINAPGTIDSDYRGEVHVPLINHGRQPFTIERGMRIAQMLI
                     TPVPTV"
BASE COUNT          603 a          934 c          825 g          492 t
ORIGIN      
        1 acctgaccga cctggagacc gtcatccagg aaatccttca ggacaagatc gcgatcgact
       61 acgaagccag cggcctgatt cagccggagt gattgcggtt aattgaacca cgaagacacg
      121 aagagcacga agcggaaaag atgagtttta gccgcagatt tcgcagatcg aagagaagcc
      181 tttttcgaat ctgcatcatc tgtgaaatct gtggttaaaa cacctttgtc tttccttcgt
      241 gctcttcgtg ccttcgtggt tacaatgttt tcatgagcac cgacgcgcaa acgcagtcgc
      301 tgaagggccg cgagatcatc gtcgccgtct gcggtggcat cgcggcgtac aaggtcgccg
      361 acgtcgtctc caaactcgtc cagcgcggcg ccggcgtcag cgtggtcatg accgacgacg
      421 cccagcgctt cgtcgcgccg ctcaccttcc aagccctctc aggccgggtc gttcgcacca
      481 gcacgtttga actgcccgac agttccgacc cgcagcacat cagcctgagc gaacgagccg
      541 acctgatgct cgtcgccccc gccacggcga acatcatcgc caaggccgct catggcgttt
      601 gcgatgaact ggtttcgctg atgatctgcg ctgccgcctg cccggtcgtc ttcgcgcccg
      661 cgatgaacga ccgcatgtgg gccaacccca tcgcgcagga aaacgtcgcg aagctcaagt
      721 tgcatggcta tcgcttcatc ggtcccgaag ccggctggct cgcctgccgc aacgtcggcg
      781 ccggtcgatt ggccgaaccg gccacaatcg tcgatgacgt ggccgcaatc ctcgcacatt
      841 ccgcgctccc acccgcgact tcggatgata gaaccatcca ctcacgctcc agcccctgaa
      901 cacttaaccg aggtcgcagc atggccgaga agcagatcga tcggaaacgt ctcatcgaat
      961 tgctgaacga agacctgtcg cgcgaatatc aggccgtcat cgcctacgtc gtgtacagca
     1021 aggtgatcaa aggcgcggcg tacatgaata tcgccgccga gttggagaag cacgcgggtg
     1081 aagagctgga acacgcgctg aagatcgccg accagatcga ctatctcggc ggcatgccgg
     1141 tcaccaaacc gcaaccggtg aagacgtcgg aaaaggcgga ggacatgctg cggttcgatc
     1201 tggaaaacga gcgcgtgacg atcgccaact accgcacccg cgtccgccag gccgacgcct
     1261 tgggcgagtt cgcgctgagc gaaaccctcc gcgagatcct ggtgcaggag caggagcacc
     1321 tgaccgacct tgcggcggcg ctgggcatcg acgcgcccga tccaggcgag gcgtgaaaaa
     1381 cgcgcgtgaa atcgcgcccg acgccggtgt cggaggtact cggaagaccc gggctcgatc
     1441 gcacgaagaa ccctgcctct tcggagtacc tcagacgccg gcgtcgaaaa tccaactcgt
     1501 acatttttcc gccacgacca tcctctagcc cgtcgtcgca acgactgcaa aacctgcaaa
     1561 ttctccgcca atttccgccc cgccgccggc gtgggtagaa ttccatccat gcgcgtcctg
     1621 atcaccgcgg ggccgacccg cgaaccgatc gatcccgtcc gctacatcac caaccgcagc
     1681 agcggttaca tgggcaaagc cctggccgac gcggcgatta aagccggtca ttcgacgacg
     1741 ctgattttcg gtcctgtcaa catcacgacg ccgaccgaag cgcggcggat cgatgtcgac
     1801 accagccggc agatgtacga cgcggtgatg gctgaatttc ccaaccacga cctgctgatc
     1861 atggccgccg ccgttgccga ctacaggccc cgccgcattc gcaacgagaa gctcgaacgc
     1921 ggcggcacgt tggtcgtcga attcgaagcg accgaagaca tcctcgccga agcggggaag
     1981 attaaacggc tcgatcagcg gacggtcggc ttcagcctcg tcagccgcgg ggatttggcc
     2041 cggtcaatgg aaaaactgcg gcgaaaaaat ctcgacctga tcgtctacaa caccttcgaa
     2101 acgcagggca gcaaatcgat cgaatcggtg ctgctgtggc ccagcgggcg cagcgaggaa
     2161 ctcccctcgc gaagcaaggc cgacctggcc gatattctcc tcaagcgggc agctgaattg
     2221 ttcgcgtcaa cggattgacg cgcaagccgc gcgccagttt cgcaagcgcc tcatcaaatg
     2281 ccaacgcccc catttagccc ggcgtgatta cacgccgcgt ttacgtggat cattcaggcc
     2341 cgtcgattaa gaaatcgaac ggcctcgatt tcccaaggta accccgcgtg tgttcacgcc
     2401 gggctaaatg gggtgtttga cgttcgatgg tgcttgcgaa aagcaacgga gtgcttctct
     2461 tgccgggccg gtcgccgtca cgtccgataa ccgttcgcat caagcggacc gcacataccg
     2521 cggacgtccc cttgccgacg aagatgaccg agcacgccgc cgggttcgac ctggcggccg
     2581 cggtggcgtc gccggtggtt ttgcagcccg gcgaaatcca gctgatcccc tgcggtttcg
     2641 caatggcggt gccgcacggt tacgaagcgc aggtccgccc gcgcagcggg ctgtcgagca
     2701 agcacggcgt caccatgatc aacgcccccg gcaccatcga cagcgactac cgcggcgaag
     2761 tccacgtgcc gctgatcaac cacggccgac agccgttcac gatcgaacgt gggatgcgca
     2821 tcgcgcagat gctgattacg ccggtgccga cagt
//
LOCUS       DATIMH010000970         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4856580, whole genome shotgun sequence.
ACCESSION   DATIMH010000970 DATIMH010000000
VERSION     DATIMH010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4856580"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VLI90_16855"
     CDS             <1..469
                     /locus_tag="VLI90_16855"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HSV15933.1"
                     /translation="PQRDEVLKLYRAHAKGLASLQSGSGLWHQMLDRSDSYLEESCSA
                     MFTYAIARGVNRGWLSGEAYGPVAISGWNGVLTQVTADGHLKNVCPGTSYADDYVYYY
                     HRPAVDDIHGYGPTLLAGAEIIKLLKNDHYRFEVGGNAPLYVLDKKKAPASRE"
     gene            702..1478
                     /locus_tag="VLI90_16860"
     CDS             702..1478
                     /locus_tag="VLI90_16860"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSV15934.1"
                     /translation="MAGAFNPAQFDARAWAHTARSAGMKYVVITAKHHDGFCMFDSKL
                     TKYDIVDATPYARDPMKDLADAVRAQGMTFCFYYSVTDWHNRDFPAKYSQHGFHGDPT
                     PDADPEKYVAYVKGQVRELLTNYGPIGILWFDGGGSFGKNNDARIHVMHAQEIIDEIH
                     QLQPNCLVNNRLGLPGDYGTPEQKIPGGKSDAPFEVCMTLNKHWGYNRADNNWKDTST
                     VIHNLLDIVSKGGNYLLNVETTADGLIPQPAIDILADVGR"
     gene            1479..1775
                     /locus_tag="VLI90_16865"
     CDS             1479..1775
                     /locus_tag="VLI90_16865"
                     /inference="COORDINATES: protein motif:HMM:NF028067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HSV15935.1"
                     /translation="MDKNGESIYGTTASPLDAAPKWGRVTQKDKKIYLHIFDWPSDGK
                     LNIEGLPAADYQASLLADANHSALKAERDGKTLAIELPSAAPDPIDSVIVLESK"
     gene            1999..2844
                     /locus_tag="VLI90_16870"
     CDS             1999..2844
                     /locus_tag="VLI90_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15936.1"
                     /translation="MPTVDWSKLKPSDFRDDELDIPFFLDHFHELLDGIQETGPERGW
                     INIRVWRGQGQQHSYNARVMESYLAVAHFYCTDRPWNIYRGSPAVRQRLEAMFEYWLT
                     LQSKDGEFSEYGPGRYNLPATAFATKFMGQTLTLLKDGPPIDPDLLKRVTEADRKAII
                     ATFIIPELYAHAKIYTNQYSNVFAGAPAYIALHPDAEITRLLNEVFARSARDFMSPAG
                     FFYEHDASDFGYTLHTTNSDMTMAYFYLKGTPLGELIEQEEARWVDWLGSATTSCASR
                     TVRRS"
     gene            2781..>3759
                     /locus_tag="VLI90_16875"
     CDS             2781..>3759
                     /locus_tag="VLI90_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15937.1"
                     /translation="MGGLAWLSYNLLREPDGSTFVINRCMESRQRHLDWSRQQSPMAE
                     KVELARAFASSIEERAAQAAQARAQLETDWPHFPPLDGREFGPYSFVHRTHFNWFPTE
                     AQRAAAIAQLPYIARDSFVHQRADDRFPLVCTYVRRPTYYAAFDAGTHRSDPKRFGLG
                     IIWNPSAGAVFQSPTGTDVACWGTRLGDAKTPSEAGDLTAEYHVGEDTIEPEPGHRDL
                     PIGTLSVRYPLQADGEKTITFNVGNIAVAAKHAGAMTEQLPLLAPDGRVTIDDGRATV
                     AYDGATLATANQIAVQLYTSSRADTWLLSRHETSPHHTSRFRRHRHRDDR"
BASE COUNT          769 a         1191 c         1132 g          667 t
ORIGIN      
        1 cccgcagcgc gacgaggtgc tgaagcttta tcgcgcccat gccaagggcc tggccagctt
       61 gcagtccggc agcggactgt ggcaccaaat gctcgatcgt tccgattcgt acctggagga
      121 atcgtgcagc gcgatgttca cgtacgcgat cgcccgcggc gtcaaccgcg ggtggttgag
      181 cggcgaggca tacggtcccg tggcgatcag cggctggaac ggtgtgctca cgcaggtgac
      241 cgccgatggt cacctgaaga acgtctgccc cggcaccagc tacgcggatg attacgttta
      301 ctactatcac cgccccgccg tcgatgacat ccacggttac gggccgacgc tgctggccgg
      361 cgcggagatc atcaagctgc tgaagaacga tcattatcgc ttcgaagtcg gcgggaacgc
      421 tccgctgtat gtgctggata agaagaaagc accggcgtcg cgggaataac cgccgtcatc
      481 ctgagcgaag cacgtactcg ggcgcagtcg aaggatctgg cctcgacgtc gattaacctt
      541 cgcttcatca agtgttcagg gactcctggg ctagatcctt cgagtccggc gagtgcgcct
      601 gcgctggagg atgaccagcc agagggagaa tcacatgaac acgcgaatcg cgatcgtagc
      661 tctgctggca ctcctcacca ccgtcgcccg aggcgccgac gatcgccggc gcgttcaatc
      721 cggcgcaatt cgacgccagg gcgtgggcac acaccgccag gtctgccggc atgaaatacg
      781 tcgtcatcac ggccaagcac cacgacggct tctgcatgtt cgacagcaag ctgacaaaat
      841 acgacatcgt tgacgccacg ccgtacgccc gcgatccgat gaaggacctg gccgacgcgg
      901 tgcgcgccca gggcatgacg ttctgcttct actattccgt gaccgactgg cacaaccgcg
      961 acttccccgc gaagtattcg cagcacggat tccacggtga tcccacgccc gacgccgatc
     1021 cggaaaaata tgtggcgtac gtcaaaggcc aggtgcgcga gctgcttacc aactacggcc
     1081 ccatcggcat cctgtggttc gacggcggcg ggtcgttcgg caagaacaac gacgcccgca
     1141 tccacgtcat gcacgcgcag gagatcatcg atgagatcca ccagcttcaa cccaactgcc
     1201 tggtgaacaa tcgccttggc ctgcccggtg attacggcac gcccgagcag aagatccccg
     1261 gcggcaagag cgacgcgccg ttcgaagtct gcatgacgct caacaagcat tggggctata
     1321 accgcgcgga caacaactgg aaagacacga gcaccgtcat ccacaatctc ctggacatcg
     1381 tgagcaaggg cgggaactat ttgctgaacg tcgagacgac ggccgatgga ctgattccac
     1441 aacctgcgat cgacatcctc gccgacgtcg gccgctgaat ggacaagaac ggcgagtcaa
     1501 tctacggcac cacagcgagc ccactcgatg cggcgccaaa gtggggacgg gtcacgcaga
     1561 aggacaagaa gatttacctg cacatcttcg attggccgag cgatggaaag ctgaacatcg
     1621 aagggcttcc ggcggcggat tatcaagcgt cgctgctcgc ggacgcgaac cattcggcgc
     1681 tgaaggcgga gcgagacggc aagacactgg cgatcgagtt gccttcagcg gcgcccgatc
     1741 caatcgattc cgtcatcgtt cttgagtcga agtgacggcc tgtcaaagca acaaggatgc
     1801 gtttcgatgt tgcaagatgt ttttattagc cgcaggcgac agccagcggg gggtttcgat
     1861 attaatgacg cgtgtcttcc aacccccgct ggctgtcgcc tgcggctatc aaagatactc
     1921 cctccgcgcg tcgcgcggat cgcttcgatc tgcctctttc ttctcgttcc ttccatcgca
     1981 tccgcagctt gggacaaggt gccgacggtc gattggtcga agctgaagcc atccgatttc
     2041 cgcgatgatg aattggacat tccgttcttc ctcgaccact tccacgagct gctcgacggc
     2101 atccaggaaa ccggcccgga gcgcggttgg atcaacatcc gggtctggcg cggccagggc
     2161 cagcagcatt cgtacaacgc gcgcgtgatg gagagctatc tcgctgtcgc gcatttctat
     2221 tgcaccgatc ggccctggaa catctatcgc ggttcaccgg ccgtgcgcca gcggctggag
     2281 gcgatgttcg aatactggct cacgctgcag agcaaggacg gcgagttcag cgagtacggc
     2341 cccggcagat acaacctgcc cgccaccgcg ttcgccacga agttcatggg gcagacgctc
     2401 acgctgttga aggatggacc gccgatcgat ccggacttgt tgaagcgcgt cacggaggcg
     2461 gatcgcaagg ccatcatcgc gacgttcatc atccccgagc tgtacgccca cgcgaagatc
     2521 tatacgaacc aatacagcaa cgtcttcgcc ggcgcaccag cctacattgc gctgcatcct
     2581 gacgcggaaa tcacgaggct gctgaacgaa gtctttgccc gcagcgcccg cgacttcatg
     2641 agccccgccg ggttttttta cgagcatgat gcatcggact tcggctacac gctgcacacg
     2701 accaacagcg acatgacgat ggcgtacttc tacctcaaag gcacgccgct gggtgagctg
     2761 atcgagcagg aggaagcgcg gtgggtggat tggcttggct cagctacaac ctcctgcgcg
     2821 agccggacgg ttcgacgttc gtgatcaacc gctgcatgga atcgcggcag cggcacctcg
     2881 attggtcgcg gcaacagagc ccgatggcgg agaaggtcga actggcgcgg gcgtttgcat
     2941 cgtcgatcga agagcgtgcg gcgcaggcgg cacaggcgcg ggcgcagctg gagacggatt
     3001 ggccgcactt cccgccgctg gatggccgcg agttcgggcc gtattccttc gttcaccgca
     3061 cgcacttcaa ctggtttccc accgaagcgc agcgcgcggc ggccatcgcg cagctgccgt
     3121 acatcgcgcg agattcattc gtccatcagc gcgccgacga ccgctttccg ctggtctgca
     3181 cgtacgtccg ccgaccaacc tattacgccg cattcgacgc cggcacgcac cgcagcgatc
     3241 cgaagcggtt tgggctgggc atcatttgga acccgagtgc cggcgcagtc ttccaatccc
     3301 caaccggcac cgatgtcgcg tgctggggca ctcggttggg cgacgcgaaa accccgagtg
     3361 aagcgggcga tctgacggcg gaatatcacg tcggcgagga caccatcgag cctgagccgg
     3421 gtcaccgcga tctgcccatc ggcacgttgt ccgtccgcta tccccttcag gcggatggcg
     3481 agaagacgat cacgttcaac gtcggcaaca tcgcggtggc cgcgaaacat gctggcgcga
     3541 tgacggagca actgccgctg ctggcgccgg acggacgcgt aacgatcgac gatggccgtg
     3601 cgacggtggc gtacgacggc gcgacgctgg cgaccgcgaa tcaaatcgcc gtccaacttt
     3661 atacttcatc ccgcgccgac acatggctac tgtcccggca tgaaacgagt ccgcatcaca
     3721 catcccgttt tcggcgtcat cgtcaccgcg atgatcggc
//
LOCUS       DATIMH010000971         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3407869, whole genome shotgun sequence.
ACCESSION   DATIMH010000971 DATIMH010000000
VERSION     DATIMH010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3407869"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VLI90_16880"
     CDS             <1..1189
                     /locus_tag="VLI90_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSV15938.1"
                     /translation="LLQAHGANVLGRWSHRISDESDFAVQMYYDHLDREPLGSPYHIN
                     TGDIDGQYRFPMGARQSIICGADFRYWADNLAPGPIATFVPQVRDSYLVNGFIQDDLT
                     VVPDRLHLIAGTKLEDNSQSGFEYQPSGRLLWTPNESNSAWLAVSRAVRTPARWEQDA
                     RISPIPSSSPLVPLPVAPQARGNPGFDSETLLAYEAGYRVKPSSTFSVDAAAFLNSYD
                     NLRGFDTGAPSFIPTPVPHLLVPITVANSFYAESYGAELSANWQVNEHWRLAGSYSFL
                     QVQAHQHKAASAANDDQERMLESSSPEHQAQLHSYWDITRDLQLNMSLYYVDRLSALN
                     VPSYIRGDVGVTWTPRKNLVLSLVVQNVFDNHHPEFQSSGTTLVTSSEVPRTMYGQVV
                     FRY"
     gene            1228..1782
                     /locus_tag="VLI90_16885"
     CDS             1228..1782
                     /locus_tag="VLI90_16885"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HSV15939.1"
                     /translation="MSMSRLARSKFVFAAVAAVLLFRADAARAEPSREYAVKAAFIYN
                     FAQFTQWPADAFASPDSAFVIGVIGGDPFDGALEKAVAGKTIAGHPMVVKQVDNVADV
                     GACALVFVPASQEARVSEVLGAVGDKPILTVGETDNFPWAGGVIRFVTEDNKVRFEIN
                     SDAADKARLRISSKLMKLARIFKR"
     gene            1800..>2961
                     /locus_tag="VLI90_16890"
     CDS             1800..>2961
                     /locus_tag="VLI90_16890"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSV15940.1"
                     /translation="MTTLGNIQPPTPTMPEGTRPQGWGTKTRRSSIRGKLIALCMASA
                     AVALLVACSVFLAYDYRQFKINIVDSMSNFADIVGGNCTAAIQFNDPESAGGTLATLS
                     RQSEVEAAAIFTNSGELFAKYARANHTGDLPTHLNFEGGRFGDGFVEVVQPIELNGKI
                     IGSSYVRCDLHQLHEHMRNYGLVMLIVLAGTMGMAYMLANRLQRVISSPILTLTSTAR
                     TVSSAKDYSARVENNGRGDELGVLIDCFNEMLAQIQQRDVELIRHREHLEEEVQARTL
                     ELRKTNTDLTTAKEKAEVASAAKSNFLANMSHEIRTPMTAILGYADLMLAPQQTMSDR
                     VNCLHVIRRNAKHLMELINDILDVSKIEADKMTVESIECDVARVIVDVSSMLR"
BASE COUNT          587 a          892 c          924 g          558 t
ORIGIN      
        1 gctgctgcaa gctcacggcg cgaatgtgct gggtcgatgg agccaccgca tttcggacga
       61 atcggatttt gccgtccaga tgtattacga ccacctggac cgcgaaccgc tgggcagccc
      121 gtaccacatc aataccggcg acatcgacgg gcagtatcgt tttccgatgg gcgcgcggca
      181 gtccatcatc tgcggcgctg atttccggta ctgggccgac aatcttgccc ccggaccgat
      241 cgccaccttt gtgccccagg tgcgcgacag ttatctggtg aacggcttca tccaggatga
      301 tctgacggtc gtgccggatc gtttgcacct gattgccggt accaagctgg aagacaatag
      361 ccaaagtggt tttgagtatc agccatcggg ccgcttgttg tggactccaa acgaaagcaa
      421 ttcggcgtgg ctggcggttt cgcgcgccgt gcggaccccg gcgcgatggg agcaggacgc
      481 ccgcatttcg ccgattccaa gctccagccc tttggtgccg cttccggtgg cgccgcaggc
      541 ccgggggaat cccggtttcg attctgaaac attgctggcc tacgaggcgg gctatcgcgt
      601 gaaaccgtcg tcgacgttct cggttgacgc agcggctttt ctaaacagtt acgataacct
      661 gcgcggcttc gacacgggcg cgccgtcgtt cattcccacc ccggtgccgc acctgcttgt
      721 gcccatcacg gtcgccaaca gcttttacgc cgaaagctac ggcgcggagc tttccgccaa
      781 ctggcaggta aacgagcact ggcgattggc cggttcctac agcttcctgc aggtgcaggc
      841 tcaccagcac aaagcggcca gcgctgccaa cgacgaccag gagcgcatgc tggagagctc
      901 cagccccgag caccaggcgc agctgcattc atactgggac atcacgcgcg accttcagtt
      961 gaacatgagc ctgtactacg tcgatcggct ttcggccctg aacgtgccgt catacattcg
     1021 cggcgacgtt ggtgtgactt ggaccccgcg gaagaacctg gtgttgtcgc tggtggtgca
     1081 gaacgtcttt gacaaccacc atccggaatt ccaaagcagc ggcacaaccc tggtcaccag
     1141 tagcgaagtg ccgcggacga tgtatggaca ggtcgtgttc cgatactgat cccgatactg
     1201 atcccgagac tgattagcga aagctgaatg tcgatgtcgc gtctggcccg ttccaagttc
     1261 gtctttgccg ctgtggcggc ggtgctgctg tttcgcgcgg atgctgcgcg ggcggagccg
     1321 tcgcgcgaat acgcggtgaa ggcggcgttc atttacaact tcgcgcagtt cacgcagtgg
     1381 cctgccgatg cgtttgcgtc gccagacagt gcgttcgtga ttggcgtcat cggcggcgac
     1441 ccgttcgacg gcgcgctcga gaaagccgtg gccggcaaaa ccatcgccgg tcacccgatg
     1501 gtggttaagc aggtggacaa cgtcgccgac gttggcgcgt gtgcactggt tttcgttccc
     1561 gcttcgcagg aggcgcgcgt gagcgaggtg ctcggcgcgg tgggcgacaa gccgatcctg
     1621 acggtcggcg agaccgataa cttcccctgg gccggtgggg taattcgctt cgtcacggaa
     1681 gacaacaagg tgcggttcga gatcaattca gatgcggccg acaaggcgcg gctgcggatc
     1741 agctcaaagc tgatgaagct ggccaggatc ttcaaacgct gaggaaacga ggaattgcga
     1801 tgacgacgct gggcaacatt caaccgccga cgccgacaat gccggagggc acgcggccgc
     1861 aaggctgggg cacgaagacg cggcgctcga gcatccgcgg gaagctgatc gcgctgtgca
     1921 tggccagtgc cgccgttgca ctgctggtcg cgtgctcggt cttcctcgcg tacgactacc
     1981 ggcagttcaa gatcaacatc gtcgactcca tgtcaaactt tgccgacatc gtcggcggga
     2041 actgcaccgc ggcgattcaa tttaatgacc ctgaatcggc cggcggcacg ctggcgacgc
     2101 tgtcgcgcca atcggaagtg gaagcggcgg ccatcttcac caacagcggc gagttgttcg
     2161 ctaagtacgc ccgcgcgaat cacaccggtg atcttcccac gcatctgaac tttgagggcg
     2221 ggcgcttcgg tgacggcttc gtcgaagtcg tccagccgat cgagttgaac ggcaaaataa
     2281 tcggctcatc gtatgtccgc tgcgatctgc accagctgca cgagcacatg cggaactatg
     2341 gcctggtcat gctgatcgtg ctggccggca cgatgggcat ggcgtacatg ttggccaatc
     2401 gcctgcagcg ggtgatctcc tcgccgatcc tgacgttgac cagcaccgcg cgcacggtct
     2461 ccagcgcgaa agattattcc gctcgcgtgg aaaacaacgg gcgaggcgat gagctgggcg
     2521 tgctgatcga ttgcttcaac gagatgctgg cgcagatcca acagcgcgac gtcgagctga
     2581 tccgacatcg cgagcacctg gaagaggaag tgcaggcccg cacgctggag ctgcgcaaga
     2641 ccaacaccga tttgacgacg gcgaaggaaa aggcggaagt cgccagcgcc gcaaagagca
     2701 acttcctggc caacatgagc cacgagatcc gcacaccgat gaccgcgatc ctcggctacg
     2761 cggacctgat gctcgctccc cagcagacga tgagcgaccg cgtgaattgc ctgcacgtca
     2821 tccgccgcaa cgccaagcat ctgatggaat tgatcaacga catcttggac gtgtcgaaga
     2881 tcgaagcgga caagatgacg gtcgagagca tcgaatgcga tgtcgcccgc gtgatcgtgg
     2941 acgtcagcag catgctgcgg c
//
LOCUS       DATIMH010000972         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1970860, whole genome shotgun sequence.
ACCESSION   DATIMH010000972 DATIMH010000000
VERSION     DATIMH010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970860"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1284)
                     /locus_tag="VLI90_16895"
     CDS             complement(226..1284)
                     /locus_tag="VLI90_16895"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSV15941.1"
                     /translation="MLPISDSDLHNQSQPAQAANADAPLLDAYSRAVTSAVNTVAPSV
                     VHIHVRAHSDGGRSHGGSGSGVIFTPDGFILTNSHVVHNAWQLRVALADGNEYGAQLI
                     GEDPHTDLAVIRLTGTSNGVSTMSESDTPADAARKAGFPAATLGDSSALQPGQLVIAI
                     GNPLGFQATVTAGVVSAVGRTFRSHSGRMIDNVIQTDAALNPGNSGGPLVDSSGRVVG
                     INTAVIAYTQGLCFAVPINTAKFVATQLLAHGRVSRSYIGVMGQTVPIPRRLTRFWKL
                     NQTTGILVAGMEDHSPAAHAGLEEGDILLSFNHHPVKDIDALHRLLTADFIGKPSPVT
                     VLRGYDKLELTLTPGEAK"
     gene            complement(1435..>1804)
                     /locus_tag="VLI90_16900"
     CDS             complement(1435..>1804)
                     /locus_tag="VLI90_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15942.1"
                     /translation="PAIFGITRDPFIVFTSNVFAILGLRSMYFLLAGVMDKFHLLKYG
                     LAAVLSFVGVKMLLPGLSELYGHLTHSTHSWEIDKNVSLLVIVICLAVSIVASLLFPA
                     KARHQNPLADEGPRGIAPAK"
BASE COUNT          344 a          514 c          614 g          332 t
ORIGIN      
        1 ctgctgcacg cgatcgatcg gaataagtcc ggcagggcgt cttttgacgt tcggcatcgc
       61 actccatctc cccgtggttt caggaggcgg tcacaaattg tgaccacctt tcgtggttgt
      121 ttcgcggtgc agattgtgac cttaaactgc gcatttgcca acataaagtc gcaatttgcg
      181 acctcaggtt ggaaggcccg ggacaaccat cgaaaagatg tgtgcttatt tcgcctcacc
      241 cggcgtgagc gtcaattcca atttgtcata accccgcagc accgtcaccg gcgacggctt
      301 gccaataaaa tcagcggtca gcaggcgatg cagcgcgtcg atatccttca ccgggtgatg
      361 gttgaacgac agcagaatgt cgccttcttc cagccctgcg tgggcggcgg gggagtggtc
      421 ttccatgccg gcgacgagga tgccggtcgt ctggttgagt ttccagaagc gcgtcagccg
      481 ccgcggaatc ggcaccgtct gccccatcac gccgatgtat gagcgcgaca cccggccatg
      541 cgcgagcagt tgcgtcgcga caaacttggc ggtgttgatc ggcacggcga agcagagccc
      601 ctgcgtgtag gcgatcaccg ccgtgttgat gcccaccacg cggccgctgg aatcgaccag
      661 cgggccgccc gaattgcccg ggttcagcgc cgcgtcggtc tgaatgacat tgtcgatcat
      721 gcggccggag tgcgagcgga aggtgcgtcc cacggcggag accacgcccg cagtcaccgt
      781 cgcctgaaag cccagcggat tgccaatggc gatcacgagc tgccccggct gcagcgcgga
      841 ggagtcgccg agcgtggcgg cggggaatcc ggctttccgc gcggcgtcgg cgggcgtatc
      901 gctttcggac atcgtcgaga cgccattgct cgttccggtg aggcggatga ccgcgagatc
      961 ggtgtgcggg tcttcgccga tgagctgcgc gccgtactca ttgccgtcgg cgagggcgac
     1021 gcggagttgc caggcgttat gcacgacgtg ggagttggtg agtataaagc catcgggtgt
     1081 gaagatcacg ccggagccag agcccccgtg agaccgcccg ccgtcggagt gggcccgaac
     1141 gtggatgtgc acgacggacg gcgcaacggt attgacggcg gaggtgacgg cgcgggagta
     1201 cgcgtcgagc agcggtgcat cggcgttcgc ggcctgcgcc ggctgggatt gattgtgaag
     1261 gtcagagtcg gaaatcggga gcatggttga gcctctatag acgtttatct atggtaggcg
     1321 cggggttgga ggaatcaaat tgagcgcggc cggagggtgg atgcggggga agcctgcgtg
     1381 ggcatctcct atcgcgggcg ttccctcgcc ggggccgcgg actgacgcag gtgcttattt
     1441 ggcgggcgcg atgccccgcg gcccttcatc ggcgagcggg ttttggtggc gcgctttggc
     1501 gggaaacagc aggctggcca caatggagac cgccaggcaa atgacaatca ccaacagcga
     1561 cacgttcttg tcgatctccc aggaatgcgt gctgtgcgtc aagtggccat aaagttcgct
     1621 caagccgggc agcagcattt tgacgcccac gaatgaaagc accgcggcca agccgtattt
     1681 gagcagatgg aacttatcca tgacccccgc cagcaggaaa tacatcgagc gcagcccaag
     1741 gatggcaaac acgttggacg tgaaaacgat aaacgggtct cgcgtgatgc cgaagatagc
     1801 gggg
//
LOCUS       DATIMH010000973         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_812659, whole genome shotgun sequence.
ACCESSION   DATIMH010000973 DATIMH010000000
VERSION     DATIMH010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812659"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..>2548)
                     /locus_tag="VLI90_16905"
     CDS             complement(283..>2548)
                     /locus_tag="VLI90_16905"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSV15943.1"
                     /translation="GIDFGPVKGNLSAANVFPFDANGSAPGTQQDLNGDGNLDVGGSS
                     NASVTGFFDARAGSVISTPPGNDVSGSLELEVGTLTFTATALNFGGESDINFVPQAPV
                     GGNLSAVWREDNNAFNNSTGMFAGGTPFKITSPAAFPQGPIAVDDTAAAAPGVQQSID
                     VLQNDFDHFGTIDPASVTILGAPNHGGTAVVDTTTGAITYTPDVNFIGTESFTYTVTD
                     NQGHTSPAGTVTATVSLANLAAPVANDDSANTVLGTPISVDILSNDTASAPATIDASS
                     IILGGTVVGGTPSIQPDGTVLFTPDPNFIGAGFFTYTVNDTNGQTSNIARAGVNVGVD
                     ITPVVGGHRVATFSDPDGTNAVVTLNRGTATLQFNGTAQSSVARSGAVTIFNGSADFG
                     LASVTMTGTSAASVLAINARGGADGAISVGSISTVAGASVGVISARTTNLTGNIDVGG
                     VNVLLLNNVADAAQIQIGAAGSPALLLGDVGSASISSGVPLRLLSVRSWTDPVNESLI
                     SAPSVVTIVSTHDFASSIDITSGSLGVARIGGALAGGAWTIGGPARAIIALGGVSPAW
                     IGSIDGALGTFSVLGGSGFASSLDAGSISVFNVAGDLSGHLTVGSARVIRATGAISNA
                     IISSAGDITALIAGSMTGTSVTAGGVSNDFTAITTAAELGSNAIRAVVLTSRAANAFS
                     NSTIMAQTITTAVLGSVDTTAVGGLAAAKLGNVTFNVSGSPVHLGPAQLATVGDIKSA
                     GAVDFGNIAIRVLG"
BASE COUNT          429 a          833 c          852 g          434 t
ORIGIN      
        1 gtcaatctct tcatcaaccg cgccgggggc caagccgtag ttgacggtgc gataagacgg
       61 atcagacgca ggcgaagtcg gaggcgtggt cgtcatggac atgcgcaggt cctcgttcct
      121 gccaacgatt gtaggagttg cgccaggtca ttgccagaga acgccagctt cgccacaaag
      181 cgccagttcg gcgggcaacg ctttaatagc cgggggcgga agccccggtc ttttcgatcg
      241 tgaaagaccg ggccttccgg ccccggctat caaggatatg gcctaaccca acacgcgaat
      301 cgcgatattc ccgaaatcca ccgcaccggc gctcttgatg tcccccaccg tcgccagctg
      361 cgccggcccc agatgcaccg gcgatcccga tacgttgaac gtcacgttgc caagcttcgc
      421 cgcggccaaa ccaccaacgg ccgtcgtatc aaccgagccg agcaccgccg tcgtgatcgt
      481 ctgcgccatg atcgtggagt tcgaaaacgc gttcgcggct ctactcgtca acaccaccgc
      541 gcggatcgca ttgctgccca gttccgccgc cgtggtgatg gctgtgaaat cgttgctcac
      601 gccgccggcg gtcacgcttg tgccggtcat cgacccggcg atgagcgccg tgatatcacc
      661 ggccgaactg atgatcgcat tggagatggc gccggtggcg cgaatgacgc gggccgagcc
      721 gaccgtcagg tgaccgctga gatcaccggc gacgttgaag acgctgatgc tgccggcgtc
      781 gagcgacgat gcgaacccgc tgccaccgag cacgctgaag gtgccgaggg cgccgtcgat
      841 gctgccgatc cacgctggcg acacaccgcc cagcgcgatg atagcgcggg cgggaccgcc
      901 gatcgtccac gccccgccgg ccagcgcgcc accgatgcgc gcgacgccca ggctgccgga
      961 ggtgatgtcg atgctcgagg cgaagtcgtg ggttgaaacg atcgtcacca ccgagggcgc
     1021 ggagatcagg ctttcgttca ccggatccgt ccaggatcga acgctgagca accgcagcgg
     1081 cacaccgctg gagatcgatg ccgaacccac gtcgcccagc aacagcgccg gcgagccggc
     1141 ggcgccgatc tggatctgcg cggcatcggc gacgttgttc aagagcagca cgttgacgcc
     1201 gccgacgtcg atattgccag tgaggtttgt ggtgcgcgcc gagatcacgc cgacgctcgc
     1261 gccggcgacg gttgagatgc tgccgacgga aatcgcgccg tccgccccac cgcgggcgtt
     1321 gatcgccagc acgctggccg ccgacgtgcc ggtcatcgtc acgctggcca gcccaaagtc
     1381 cgcgctgccg ttgaagatcg tgaccgcgcc gctgcgcgcg acggacgact gggcggtgcc
     1441 gttgaactgc agcgtcgcgg tgccgcggtt cagcgtgacc acggcgttgg tgccgtcggg
     1501 atcgctgaag gtggcgacgc gatgtccacc gacgaccggc gtgatatcga cgccgacgtt
     1561 cacaccggcg cgcgcgatgt tcgaggtctg cccgttcgtg tcgttcacgg tgtacgtgaa
     1621 gaaaccagcg ccgatgaagt tcggatccgg cgtgaacagc acggtgccat cgggttgaat
     1681 gctcggcgtg ccgccgacca cggtgccgcc gaggatgatg ctcgacgcgt cgatcgtcgc
     1741 cggcgcggac gcggtgtcgt tgctgagaat gtccacgctg atcggcgtgc cgagcacggt
     1801 gttggccgag tcgtcattcg ccaccggcgc cgccaggttc gccagcgaca cggtcgccgt
     1861 caccgtcccc gccggggatg tgtggccttg attgtcggtg acggtgtacg tgaacgattc
     1921 agtgccgatg aagttcacgt cgggcgtgta ggtgatcgcg ccggtggtcg tatccacgac
     1981 ggcggtgccg ccgtggttcg gcgcgccgag gatcgtaacg ctcgcgggat cgatcgtgcc
     2041 gaagtgatcg aagtcgttct gcaggacgtc gatcgactgc tgcacacccg gggcggcggc
     2101 cgcggtgtcg tccactgcga tcggtccctg tggaaacgcc gccggtgacg tgatcttgaa
     2161 cggcgtccca ccggcgaaca tgccggtgct gttgttgaag gcgttgttgt cctcgcgcca
     2221 cacggccgac aggttcccgc ccaccggcgc ctgcgggaca aagttgatgt ccgactcgcc
     2281 gccgaagttc agcgcggtgg cggtgaacgt gagcgtgccc acttccagtt caagcgatcc
     2341 actcacgtca ttgccgggcg gcgtgctgat cacgctgccg gcgcgggcat cgaagaaccc
     2401 ggtgacgctg gcgttgctgg agccgcccac gtcgaggttc ccgtcgccat ttaaatcctg
     2461 ctgcgtgcct ggcgctgagc cgttcgcgtc aaacggaaag acgttggccg ccgacaagtt
     2521 tcccttcacc ggtccgaagt cgatgccg
//
LOCUS       DATIMH010000974         4316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_231592, whole genome shotgun sequence.
ACCESSION   DATIMH010000974 DATIMH010000000
VERSION     DATIMH010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231592"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VLI90_16910"
     CDS             <1..919
                     /locus_tag="VLI90_16910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV15944.1"
                     /translation="IARSKFLLAMSNVDGHDIVRRSMEVCMPNIEAQGLMFSAELEAK
                     DRQLRADAGRLQQLLSNLLNNAAKFTPTGGRVTLRTRNEDDGRLFVVEVSDTGVGIDP
                     AVLPRIFNAFEQGERSITRTFGGLGLGLTIGKAIAEMHGGSLTASSEGRGRGATMTLR
                     LPVQVAAKPSADPPASQAAPPRRVSVLIVEDHAPTLRLMSRLLRSLGHEVRAAASAER
                     AVELATADCPDLLISDIGMPNQSGWWLMQELRKRCPPTMRAIAVSGYGTDDDIQRSRD
                     AGFTEHIVKPIDVQRLQAAIERAMQTSAA"
     gene            complement(1091..2278)
                     /locus_tag="VLI90_16915"
     CDS             complement(1091..2278)
                     /locus_tag="VLI90_16915"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV15945.1"
                     /translation="MKIVHIITRLIIGGAQENTLLSCEGQHDLGHDVTLITGPAIGPE
                     GSLMERATRYGYRVETIDEMRRSILPIKDFRTYRRLVRRLRELKPDIVHTHSSKAGII
                     GRRAAHAAGVPAIIHTIHGLAFTASTNRAVNHVYKMLERNTAPITDKIVCVADAMRDQ
                     SLAGHVGRPEQYVTVYSGMETAPFINPPTPREVVRRQLGLSDEHVAVGTIARLFHLKG
                     HEDLVHLAPDLCRRFPNLRFLWVGDGILRGRYQTQIDRLGLRDRFIFTGLVPPTKIPE
                     LANSMDILVHPSRREGLARALPQGQLARCPAVTYDIDGAKEGVIDGKSGFVLPPFDKA
                     QLGESLAKLLADEPLRRAMGTAGREFALGRFDAKVMVAALERVYVETLASGARSQQRE
                     SAT"
     gene            complement(2393..4198)
                     /locus_tag="VLI90_16920"
     CDS             complement(2393..4198)
                     /locus_tag="VLI90_16920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSV15946.1"
                     /translation="MAFLFRCDSLAKSFGQRQLFSGISISFEDGERTGLIGPNGSGKS
                     TLLKILAGLEQPDDGTLTMRRGLKLGYVPQQDVFSAGATVQDVMADAVDDLHIEPHDR
                     DAQIAALLTRIGFTDAEQRVDALSGGWRKRLAIARQLIRKPDVLLLDEPTNHLDLEGI
                     VWLEKLLANPPFAFLLVSHDRYFLEHSTNRTVELNAAYADGYLSINSNYSDFLEKKES
                     YLAAQASLEQSVAGRVRREIEWLKRGAQARSTKAKGRIEQAQRLMGELADLRTRNAQG
                     QTAGVDFVGTHRQTRKLLTAEKVMKSLGGRMLFKDVDLTLAPGTKLGLLGPNGSGKST
                     LIKLMAGQLEPDAGNIRRAEGLRIVLFDQSRAALDQQQTLRQALSPGSETVAFRGESI
                     HVSSWAARFLFTRQQLDMAVRDLSGGEQARILIARLMLQPADLLILDEPTNDLDITSL
                     EVLEESLADFPGALVLVTHDRYMIDRLSTEVLGLDGIGGARLFADRAQYERAKEDAQR
                     KPTEKFAAKTSTAPAKEKPKKLTYMEQREWEQIEERIQTAEAELDAKHQLLGNPAVMA
                     DHLKMADAGRAVAAAQAAVDALYRRWEELETKRGG"
BASE COUNT          720 a         1402 c         1404 g          790 t
ORIGIN      
        1 catcgcgcgc agtaagttcc tgctggccat gagcaacgtg gacggccacg acatcgtccg
       61 ccggtcgatg gaagtgtgca tgccgaacat cgaagcgcag gggctgatgt tcagcgcaga
      121 attggaagcg aaggaccgcc agctgcgcgc cgatgcgggc cggctgcaac agctgctgtc
      181 gaacctgctg aacaacgcgg cgaagttcac gccgaccggc gggcgggtga cgctgcgcac
      241 gcgcaacgaa gacgatggcc gcttgtttgt cgtcgaggtc agcgacaccg gtgtcgggat
      301 cgatccagcg gtgctgccgc gaatcttcaa cgccttcgaa cagggcgagc gcagcatcac
      361 ccggactttt ggcgggctcg gtttggggct gacgatcggc aaggcgatcg cggagatgca
      421 cggtggatcg ttgacggcgt ccagcgaagg ccgcgggcgc ggcgcgacga tgacgcttcg
      481 gctgccggtg caagtggcgg cgaaaccatc ggcggacccg ccggcttcgc aagccgcgcc
      541 gccgcgccgc gtgagcgtgc tgatcgtcga ggaccacgcg ccgacactgc ggctcatgtc
      601 gcgattgctg cgatcccttg gccacgaggt gcgcgccgcg gcgtcagccg aacgcgcggt
      661 cgagctggcg acggccgatt gcccggacct gctgatcagc gacatcggca tgcccaacca
      721 atccggctgg tggttgatgc aggagctgcg caagcgctgc ccgccgacga tgcgggcgat
      781 cgcggtgagc ggttacggca ccgacgacga catccaacgc agccgggatg cggggttcac
      841 tgagcacatc gtgaaaccga tcgacgtgca acgcctgcag gcggcgatcg agcgcgcgat
      901 gcaaacctcc gccgcgtagc acgggcgtct cgcccgtgtt tcgtggtact ccgcgaatat
      961 cggcggtacg ccgtcgatga acccatcgcg tcagcgaaga gatttcgggg accgtcgctt
     1021 gcgcgatcgc tttgccggtc gagtacggtc gccgtgcgcg agtacgcgca ccacgggcga
     1081 gacgcccgtg ctacgtggcg gattcgcgtt gctgagatct tgcgccggac gccagcgtct
     1141 ccacatacac gcgctccagc gcagccacca tcaccttcgc atcgaaccgc cccagcgcaa
     1201 actcccgccc cgcggttccc atcgcgcgtc gcagcggctc gtccgccagc agcttcgcca
     1261 gcgactcgcc cagctgcgcc ttatcgaacg gcggcagaac aaatccgctc ttgccgtcga
     1321 tgacaccttc cttcgccccg tcgatgtcgt acgtcaccgc ggggcagcgc gcgagttgtc
     1381 cttgcggcag cgctcgggcc aagccttccc gccgcgatgg atggacgaga atatccatcg
     1441 aattcgccag ctccgggatc ttcgtcggcg ggaccagccc ggtgaagatg aagcgatcgc
     1501 gcaagcccag gcggtcgatc tgcgtttggt accgcccgcg caggatgccg tcgccgaccc
     1561 agaggaaccg caggttgggg aaccgccggc acaggtcggg ggcgaggtgc accaggtcct
     1621 cgtggccttt caggtggaac agccgcgcga tcgtgccgac ggcgacgtgc tcgtcgctca
     1681 agcccagttg tcgtcgcacg acttcgcgcg gcgtcggcgg gtttatgaac ggcgcagtct
     1741 ccatcccgct gtagaccgtc acgtattgtt ccggccgacc gacgtgcccg gccagcgatt
     1801 gatcgcgcat cgcgtcggcg acgcagacga tcttgtcggt gatcggcgcc gtattgcgct
     1861 cgagcatctt gtaaacgtga ttcaccgcgc gattggtgga cgcggtgaat gccaggccgt
     1921 ggatcgtgtg aatgatcgct ggaacaccag ccgcgtgcgc cgcccgacgg ccgatgatgc
     1981 cggccttgct gctgtgcgtg tgaacgatgt ccggcttgag ctcgcgcagc cggcggacga
     2041 gccggcggta cgtgcggaaa tctttgatcg gcaggatcga gcgccgcatc tcgtcgatcg
     2101 tttcgacgcg atagccgtag cgcgtcgccc gctccatcag cgatccttcc ggcccgattg
     2161 cgggacccgt gatcagcgtg acgtcgtgac caagatcatg ctgaccttcg cagctgagca
     2221 gcgtgttttc ctgcgccccg ccgatgatca ggcgggtgat gatgtggacg atcttcatcg
     2281 gagtggaagc gtattggaaa cgtgattgcg cgcgaagcat cccagccgct tgccgcttag
     2341 cgtctgctaa tcttcccggc acgaaccgat gctacgccgc aagcggcaaa cactacccgc
     2401 cgcgcttggt ttcgagttct tcccatcgcc gatacagcgc atcgaccgcc gcctgtgccg
     2461 cggcgacggc gcggccggcg tcggccatct tcaggtgatc ggccatgacg gcggggttcc
     2521 ccagcagctg atgcttcgcg tccagctccg cctcggctgt ttggattcgc tcctcgatct
     2581 gctcccactc gcgctgctcc atgtacgtca gcttcttcgg cttctctttt gccggcgccg
     2641 tgctcgtttt cgccgcgaac ttctcggtcg gcttacgctg ggcgtcctcc ttggcgcgct
     2701 cgtactgcgc gcgatcggcg aacagtctcg cgccgccgat gccgtctagt cccagcacct
     2761 cggtcgagag gcgatcgatc atgtagcgat cgtgcgtgac cagcaccagc gcgccgggga
     2821 agtcggcgag gctttcctcc aatacttcca gcgatgtgat atccagatcg ttcgtcggct
     2881 cgtcgaggat cagcaggtcg gccggctgga gcatcagccg ggcgatcagg atgcgggcct
     2941 gttcgccgcc ggacaggtcg cggacggcca tgtccagctg ctgccgggtg aacaggaatc
     3001 gagcagccca ggacgaaacg tggatggact cgccgcggaa ggcgaccgtt tcactgccgg
     3061 gcgagagcgc ctgccgaagg gtttgttgtt gatcgagcgc cgcccgcgac tgatcgaaca
     3121 gcacgatgcg cagcccctca gcccggcgga tgttgcccgc gtcaggctcg agttgcccgg
     3181 ccatcagctt gatcagcgtg gacttcccgc tgccgttggg gccgagcagg ccaagcttgg
     3241 tgccgggggc gagcgtgaga tcgacgtctt tgaacagcat gcgaccgccg agggatttca
     3301 tcactttttc ggcggtgagc agcttgcgcg tctggcggtg cgtgccgacg aaatcgacgc
     3361 cagccgtttg gccctgcgcg ttgcgcgtgc gcaggtcggc caactcgccc atcaggcgct
     3421 gggcctgctc gatgcggccc ttggccttgg tactgcgggc ttgtgcgccg cgcttgagcc
     3481 actcgatctc ccgccgcacg cgcccggcga ccgattgctc caggcttgcc tgggcagcca
     3541 ggtacgattc ttttttttcg aggaaatcgc tgtaattact attgatgctc aaatacccgt
     3601 cggcgtacgc cgcgttcagc tcgactgttc gattcgtcga gtgctccagg aaatagcgat
     3661 catggctgac cagcaggaac gcgaacggcg ggttggccag cagcttttcc agccagacga
     3721 tgccttccag gtccaggtga ttggtcggct cgtccagcag cagcacgtcg ggcttgcgga
     3781 tcaactgccg cgcgatcgcc aggcgcttgc gccagccgcc ggagagcgca tcgacgcgct
     3841 gttcggcgtc agtgaaacca atgcgcgtca atagcgccgc gatctgcgca tcacgatcgt
     3901 gcggctcgat gtgcagatcg tccaccgcgt ccgccatcac gtcctgcacc gtcgcacccg
     3961 cggagaacac gtcctgctgc ggcacgtatc ccagcttcag tccacggcgc atcgtcagcg
     4021 tgccatcgtc cggctgttcg aggcccgcga ggattttcag cagcgtggac ttgccggaac
     4081 cgttgggacc gatcaggccc gtgcgttcgc cgtcttcgaa tgagattgag atgccgctga
     4141 acagctggcg ttggccaaac gatttggcga gggaatcgca acggaacagg aaagccatgg
     4201 aaagtccaaa tatcgtcgtg gtgtcgaaca ccctcattta gccgggcgtg aacacacgcc
     4261 gggttcacgt agatcttcgc gcacctctaa ttgcaaaatc aaaccggcgc aacttc
//
LOCUS       DATIMH010000975         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1680154, whole genome shotgun sequence.
ACCESSION   DATIMH010000975 DATIMH010000000
VERSION     DATIMH010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680154"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VLI90_16925"
     CDS             complement(<1..437)
                     /locus_tag="VLI90_16925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV15947.1"
                     /translation="MIHYPPGFRPRRGLNWGYLGLLYTSFYLCRFNLAVANKTIANEY
                     HFSYSQMSTIIFTSTLVYAFGQILNGLLTDRMGGKKAMLIGAAGTITMNILFGAASVW
                     GILGLFIAIRGVDGYFQSFGAPGMTKIKTAWFAKTERGGFAGI"
     gene            complement(568..1641)
                     /locus_tag="VLI90_16930"
     CDS             complement(568..1641)
                     /locus_tag="VLI90_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV15948.1"
                     /translation="MTAVRVGDITKNFGKTTALGGVSLQIAAGELFFLLGPSGCGKTT
                     LLRIIAGLQEPTAGRVWFNEQDVTALPTEKRNAVMCFQSYALWPHMTVRDNVRFGLDV
                     QKLSVAQRDARVEEVLHLVQMSPYADRKPTELSGGQQQRVALARALAVQPACLLLDEP
                     LSNLDAKLRHEMRGEIRRICKHAGLTTIYVTHSQEEALSIADRIAVMKDGRLAQVGTP
                     AELYHQPASAFVADFIGQTNLIMGKVVARDGGNARIETSIGIVTAHVNGQSASLPDQV
                     TLSIRPENVRIIAAAAAHIDNAAINRFAAMPLELSFLGEASEHLLQVAGQPLKAMSMP
                     PRFQLPAELTVEFDAQDVLILTQ"
     gene            complement(1761..>2778)
                     /locus_tag="VLI90_16935"
     CDS             complement(1761..>2778)
                     /locus_tag="VLI90_16935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV15949.1"
                     /translation="GNLSFGSLANATLGGISGNQNVALGNTAVSVGNNNASTTYGGTM
                     SGGGGLTKIGTGTLTLNNTNSYTGSTTVNGGAMTVATGASLASGSVSVASGGTLNVNG
                     SISQTANVTTGGTVNFAGNTSTSATLTRKLGTLTINPGGTAEVLASASTLQPEILQPS
                     SVSFGDNTSKLDLTNNELLTAGSAAAIAPSVASGQISSSTTTTSTGLGYIDQPGSTVE
                     VRYTLLGDSNLDLKVDVGDLGALATSYGVTGGVLWSNGDFNHDSKVDVGDLGALATNY
                     GSSLAANSGGSSASAASPASMVASDLAVVASGGSAAVPEPASLTLIGLGAMSLLARRR
                     RRRV"
BASE COUNT          508 a          875 c          885 g          510 t
ORIGIN      
        1 aagatgccgg caaagccgcc gcgttcggtc ttggcgaacc acgccgtctt gatcttggtc
       61 atccccgggg cgccgaagga ttggaagtaa ccatcgacgc cgcgaatcgc gatgaacaac
      121 ccaaggatgc cccaaacgga ggcggcgccg aacaggatgt tcatcgtgat cgtgccggcc
      181 gccccgatca gcatcgcctt cttcccaccc atgcgatcgg tgagcaaacc gttcaagatc
      241 tgcccaaagg cgtacaccag cgttgacgtg aagatgatcg tactcatctg cgagtacgaa
      301 aagtggtatt cgttcgcgat ggtcttgttg gcaaccgcta aattgaatcg gcacaggtag
      361 aacgaggtgt aaagcagccc aagatagccc cagttcaacc cgcggcgggg tcggaagccg
      421 ggtgggtagt ggatcacggc actggccgca gggtcttgca atgaagcaga cccgtgcccg
      481 acgcgcgcgg aactatcgct cgaatccgac agcgtatgag gtggcatgaa aagcctgtaa
      541 agcccggccg atcccgatcc ccagcgatca ctgcgtcaag attaacacat cctgtgcatc
      601 gaattccacc gtaagctccg ccggaagctg aaatcgcggg ggcatcgaca tcgccttcag
      661 cggttgcccg gccacctgca gcaggtgttc gctggcttcg cctaagaacg acaattccag
      721 cggcatggcg gcaaatcggt tgattgccgc gttgtcgata tgagcggcgg cggcggcgat
      781 gatgcgaacg ttctcggggc ggatgctcaa tgtcacctga tcggggagcg acgcggactg
      841 gccgttgacg tgggcggtga cgatgccgat cgacgtttcg atgcgcgcgt tgcccccatc
      901 gcgggcgacc accttaccca tgatgagatt cgtctggccg atgaaatcgg cgacgaacgc
      961 ggacgcgggc tggtgataca gttccgccgg tgtgccgacc tgggcgagcc ggccgtcttt
     1021 catcaccgca atccgatcgg cgatcgacag cgcttcttcc tggctgtgcg tcacgtagat
     1081 cgtcgtgagc ccggcgtgtt tgcagatgcg gcggatctcg ccgcgcatct cgtggcgtag
     1141 cttggcgtcg agattcgata gcggctcatc aagcagcaag cacgcgggtt gaaccgccag
     1201 cgcccgcgcc agcgcgaccc gctgctgctg cccgccggac aattcggtgg gtttgcgatc
     1261 ggcatacggc gacatctgca cgaggtgcag gacttcctcc acccgcgcat cgcgctgtgc
     1321 gacggacaat ttctgaacat ccagcccaaa tcgcacgttg tcgcgcaccg tcatgtgcgg
     1381 ccacagcgca tagctttgaa agcacatgac cgcattccgc ttttccgttg gcagcgccgt
     1441 cacgtcctgt tcattgaacc acacgcgacc ggccgtcggc tcttgcaatc ccgcgatgat
     1501 gcgcagcagc gtcgtcttgc cgcagccgct gggaccgagc agaaagaaca actcccccgc
     1561 tgcgatctga aggctcacgc cgccgagggc ggtcgtcttg ccaaagttct tcgtgatgtc
     1621 accaacgcgc accgcggtca tgcgggcggg attctagcgg aacggcacgc aggacaaaaa
     1681 atacggcgac ctgtcggtca ggagcaggtc gccgttgcgg aggaaaggtt gtacgcgccg
     1741 aagccgccct cgcgatgcgc tcacacgcgg cggcgacggc gacgggccag caggctcatc
     1801 gcgcccaagc cgatcagggt aagcgatgcg ggttccggca cggcggccga accaccgctg
     1861 gcgacgacgg ccagatcgct ggcgaccatc gacgcggggg aagccgcact ggcgctgctc
     1921 ccgccgctgt tggcggcgag gctcgagccg tagttcgtcg ccaacgcgcc caggtcaccg
     1981 acatcaacct tgctgtcgtg attgaagtca ccgttggacc aaagcacccc gccggtgacc
     2041 ccgtagctgg tcgccaacgc ccccaggtcg cccacgtcca ccttcaggtc caggttggaa
     2101 tcgcccagca gcgtgtagcg tacttccacg gtgctgccgg gctggtcgat gtaacccagg
     2161 cccgttgacg tcgtcgtcgt gctgctgctg atttgtccgc tggcgacgct cggcgcgatc
     2221 gccgcagccg aaccggcggt gagcaactcg ttgttggtca ggtctagctt cgaagtgttg
     2281 tcaccgaagc tcacggagct gggctgaaga atttctggct gcaaggtgga ggccgacgcc
     2341 agcacctcgg cagtgccacc ggggttgatc gtcaacgtgc cgagcttccg cgttagcgtt
     2401 gcgctcgtgc tggtgttccc ggcaaaattt acggtgccgc cggtggtgac gttcgccgtc
     2461 tgcgatatcg agccgttgac gttcaacgtg ccgcccgagg caacgctcac actgccgctg
     2521 gccagcgaag cgccagtggc aacggtcatc gcgcccccgt tgacagtggt gctgccggtg
     2581 taggagttgg tgttgttgag cgtcagcgtg ccggtgccga tcttggtcag gccgccaccg
     2641 ccggacatgg taccgccgta agtcgtgctg gcgttgttgt tgccaaccga cacggcggtg
     2701 ttgcccagcg ccacgttctg attgccgctg atgccaccga gcgtcgcgtt ggcgaggctc
     2761 ccgaagctga gattgccc
//
LOCUS       DATIMH010000976         1981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1101051, whole genome shotgun sequence.
ACCESSION   DATIMH010000976 DATIMH010000000
VERSION     DATIMH010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101051"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1981
                     /locus_tag="VLI90_16940"
     CDS             <1..>1981
                     /locus_tag="VLI90_16940"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV15950.1"
                     /translation="ALAQLTAIAQTLLRPFPPGTTPPSIVKYDASSVPILQLSLQSPA
                     MSEQELFDMGQNFIRTQLATIQGASVPLPYGGKFRSVVVDLDPEQLYAKGLSPADVSA
                     ALGNQNLILPAGTAKIGEKDYQVKLNSSPRILSEMNDLPVKVVNGATVYLRDVAQVHD
                     GYNVQTSIVRTNGSRGALLTVMRNGKASTLAVVNDVKAALPKILAGLPPELRVRQLFD
                     QSLFVRAAIKGVVREAVIAAVLTGLMILVFLGSWRSTIIVCISIPLSILVSLIIMSAL
                     GQTINVMTLGGLALAVGILVDDATVEIENTHRNMAMRKPLVRAVLDGAQQIAAPAFVS
                     TLAICIVFVPVLLLTGAAKYLFTPLAMAVVFAMMASYFLSRTLIPTMVHYMLRPEVKL
                     YARGEHGESAGGKGLIWRAHYVFNRRFELMRASYSSLLHWCLDHRAPVLVGFSVFVVA
                     SLALMVFIGRDFFPTVDSGQMRLHARAPAGTRLEETELRFAKIEDEIRQVIPPREIGM
                     ITDNIGIPNGGFNLAFGDSPTLGVSDGDILISLTAEEHGSTAEYTDRLRKRLHEKFPD
                     TVFFFEAANITNQILNFGLPAPIDVQVLGRNAADNYPIAEELKREIERIPGAADVHIH
                     QVLDYPQIQVNVDRSKAGQLGLTQRDISTSLLISLT"
BASE COUNT          365 a          580 c          638 g          398 t
ORIGIN      
        1 tcgcattggc gcaattgacg gctattgcgc agacgctgct acgtccattc ccacccggca
       61 caactccgcc cagcatcgtc aagtacgacg cgtcgagtgt gccgatcctg cagctgagtc
      121 tccagagtcc ggccatgagc gagcaggagc tgttcgacat ggggcagaac tttattcgaa
      181 cgcaacttgc gacgatccag ggcgcctcgg ttccgctgcc gtacggcggc aagttccgca
      241 gtgtcgtggt ggatctggat ccggaacagc tgtacgcgaa gggattgtcc ccggcggacg
      301 tgtcggcggc tctcggcaat cagaacctga tcctaccggc cggcaccgcg aaaatcgggg
      361 agaaggacta ccaggtcaag ctaaacagca gcccgcggat cctcagcgag atgaacgatc
      421 tgcctgtcaa agtcgtgaac ggcgcgaccg tctacctccg ggatgtggcc caggtgcacg
      481 acgggtataa cgtgcaaacc agcatcgtgc gcacgaacgg cagccggggc gcgctgctga
      541 cggtgatgcg aaacggcaag gcgtcgacgc ttgccgtcgt gaacgatgtc aaggcggcgc
      601 tcccgaaaat cctcgccggg ctgccgccgg aactgcgcgt ccggcagttg ttcgatcagt
      661 cgctgttcgt ccgtgccgcg attaagggcg tggtgcgcga ggcggttatc gcggcggtgc
      721 tgacggggct gatgattctg gtattcctgg gaagctggcg gtccacgatc atcgtctgca
      781 tctcgatccc gctgtcgatt ctggtgtcgc tgatcattat gagcgcgctg ggccagacga
      841 ttaatgtgat gacgttgggc gggctggcgc tggctgtcgg tattctggtg gacgacgcga
      901 cggtggaaat cgaaaatacg caccgcaaca tggcgatgcg caagccgctg gtgcgcgcgg
      961 tgctcgatgg cgcccagcag atcgcggcgc cggcctttgt ttccacgctg gcgatctgta
     1021 tcgtgttcgt cccggtgctg ctgctgacgg gcgctgccaa gtacctgttc acgccccttg
     1081 ctatggcggt cgtgttcgcg atgatggcgt cgtacttcct ctcgcgaaca ctgattccca
     1141 cgatggtgca ctacatgctc cggccggaag tgaagctgta tgcgcgcggc gagcacggtg
     1201 aatcggcggg cgggaagggg cttatctggc gcgcgcacta cgttttcaac cggcgcttcg
     1261 aattgatgcg agcgtcgtat tcttcgctgc tgcactggtg cctcgatcat cgcgcgccgg
     1321 tgctggtggg cttctcggtg ttcgtggtcg cttcactggc attgatggtc ttcatcggac
     1381 gcgacttttt cccgacggtc gattccgggc agatgcgcct gcacgcgcgg gccccagccg
     1441 gcacccgact ggaagagact gagctacggt tcgcgaagat cgaagacgaa attcgccagg
     1501 tgattccgcc gcgcgagatc ggcatgatca ccgacaatat cgggattccg aatggcggct
     1561 tcaatctggc attcggcgac agtccgacgc tcggcgttag tgatggggat atccttattt
     1621 cccttacggc ggaggagcac ggctcgacgg ccgagtacac tgaccgcctg cggaagcgcc
     1681 tccacgagaa gtttcccgac acggtgttct tcttcgaggc ggccaacata acgaatcaga
     1741 ttctgaactt cggcctgccc gctccgatcg atgtgcaagt tctggggcgg aacgcggcgg
     1801 acaattaccc gatcgcggag gagctaaaac gcgagatcga acggattccg ggcgccgcgg
     1861 acgtgcatat ccatcaagtg ctcgactatc cgcagatcca ggtcaacgtg gatcgcagca
     1921 aagcgggcca actgggtctg acgcaacggg acatcagcac cagcctgttg atttcgttga
     1981 c
//
LOCUS       DATIMH010000977         1803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1392563, whole genome shotgun sequence.
ACCESSION   DATIMH010000977 DATIMH010000000
VERSION     DATIMH010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392563"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..794)
                     /gene="modA"
                     /locus_tag="VLI90_16945"
     CDS             complement(24..794)
                     /gene="modA"
                     /locus_tag="VLI90_16945"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSV15951.1"
                     /translation="MRIAVLSFLAIACCARVAPAAETIDVAAAVSLREALTDASRTFE
                     LTGIHVALTLGSSGQLAAQITNGAPVDAFISAAKQQVDQLSKAGLVDDSTRQIMAGNE
                     LVLIVSPNARQTPPQNFQQLAEARFNKVAIGEPRTVPAGQYAMQTLTALQIEQALRPR
                     LIFGANVRQVLDYVERGEVAAGVVYATDAQEAGAKVRVVATADPSTHEPIVYPAVLLK
                     NAAHRDAAMRFLDFLVSDQGQAALRAHGFTHGSAPSKP"
     gene            complement(827..1222)
                     /locus_tag="VLI90_16950"
     CDS             complement(827..1222)
                     /locus_tag="VLI90_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15952.1"
                     /translation="MATRRGRRSQKKGAPPPPPRVLRGRVWVETTRGQPAITDAGADL
                     LEQVEVCGSLSEAARRLRFSYRRAWMLLDAMNHRWPGALVSTATGGRRGGGAKLTELG
                     RHVLRTYRDLQLQIEHLLDTAGDPFSPFP"
     gene            complement(1227..1457)
                     /locus_tag="VLI90_16955"
     CDS             complement(1227..1457)
                     /locus_tag="VLI90_16955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HSV15953.1"
                     /translation="MPLSARNQFAGKIVALHNGAVMSEVVIDIGGGHRIVSLISTSSV
                     KRLKLKKGGPAVAVIKATEVIISTDDPTTGGD"
BASE COUNT          310 a          545 c          609 g          339 t
ORIGIN      
        1 tgggagtacc tcagagaccg gcgtcagggt ttgctcggcg ctgatccatg tgtgaaccca
       61 tgcgcccgta gcgctgcttg cccttgatcg ctcaccagaa aatccaggaa gcgcatcgcc
      121 gcgtctcgat gtgcggcatt cttcaaaagc acagcggggt agacgatcgg ctcgtgcgtt
      181 gacgggtcgg cggttgcgac gacgcgcacc tttgccccgg cctcctgcgc gtccgtcgca
      241 tacacgacgc cggcggcgac ttcgccgcgt tcgacgtagt cgaggacttg ccgcacgttg
      301 gcgccgaaga tgagccgcgg gcgcagcgct tgctcgatct gcaacgccgt cagcgtctgc
      361 atcgcgtact gccccgcggg caccgtgcgg ggttcgccga tggcgacctt gttgaaccgc
      421 gcctccgcca actgctggaa attctgcggc ggcgtctggc gcgcattggg cgagacgatc
      481 aacaccaatt cgttgccggc catgatctgc cgggtggaat cgtccaccag ccccgccttg
      541 ctaagctgat cgacctgctg tttggcggcg gagatgaaag catcgaccgg cgcgccgttg
      601 gtgatttgag ctgccaattg cccggatgag ccgagcgtca gcgcgacgtg aatgcccgtc
      661 aactcgaacg tcctcgacgc gtcggtcagc gcttcgcgca ggctgacggc agcggcgacg
      721 tcaatcgtct ccgcggccgg cgcgacgcgg gcgcagcaag cgatggctaa aaatgagaga
      781 acggcgattc gcatccgcgg cattatatac aggagtatat aacgcttcac gggaacggcg
      841 aaaatggatc accggcggtg tccaggaggt gctcgatctg cagttgcaga tcgcggtagg
      901 tccgcaggac gtgccggccc agttccgtca gcttcgcccc gccgccgcgg cgaccgccgg
      961 ttgcggtgct gaccagcgcg cctggccagc ggtggttcat cgcgtccagc agcatccatg
     1021 cccggcgata gctgaagcgc aggcgacgcg cggcttcgga caaagagccg caaacttcaa
     1081 cctgttcgag tagatcggct ccggcatcgg tgatggccgg ttggccgcgc gtcgtctcca
     1141 cccaaacgcg cccccgcagc acgcgcggtg gtggtggtgg cgcacctttt ttctgagacc
     1201 gtcgcccgcg gcgggtggcc atcgtgttaa tccccgccgg tggtcgggtc gtccgtcgag
     1261 atgatcactt cggtcgcctt gatgacggcg acggcggggc cgcccttttt cagcttgagg
     1321 cgtttgacgg acgaggtgct gatgagcgag acgatgcgat gaccgccgcc gatgtcgatg
     1381 acgacttcgc tcatcaccgc gccgttgtgg agggcgacga tttttccggc gaattggttg
     1441 cgagcgctga gtggcatgac cggcattgtg atcggcgatg gcgcgcgggg caattgcgct
     1501 gcagtcggag gatctgcgca cgtcaaccgc ttgcggcttc gtcaaatcag gcggtgccga
     1561 ttgcgaagcc gcaagcggca caaatgctgg cgagaataaa atatcttcgc gcgaacgaac
     1621 cgccgcggga tggattgcgt aatccggtct attggtgagc aacggaatgc tcgctgatgg
     1681 aaacgggaag gctccttgcg ttgatcgatg gatcgatcgc gtcgcgacga gtcagcataa
     1741 ctggggaacg catcaaccgc ctcgggctat ggggcggttt ttgcatgcgc gggcgattgg
     1801 gcg
//
LOCUS       DATIMH010000978         8195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1971937, whole genome shotgun sequence.
ACCESSION   DATIMH010000978 DATIMH010000000
VERSION     DATIMH010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971937"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VLI90_16960"
     CDS             complement(<1..1174)
                     /locus_tag="VLI90_16960"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HSV15954.1"
                     /translation="MNFKTTIVLIVLLVAAGVALYFTREPAAKTDTSGSTEGAKLVDI
                     DAKDVNKIVVTPSDGPGYTLEKNGADWQITDPVKAPAETWQVDALVRAVTDAHTRGEV
                     DAGGANASATGLATPKYQVQLITTSKTVNLAVGDTSAVGGNLYVKLGDKSQADMIPTD
                     LNDKLAKGLDDLRKKTLVTASSADIKQITITGPNGKIALQKNGANWQITEPAPMPADD
                     AAASDITFGLTGLRADSFVDPKTVPSTVMAKPQLTVAFTSAAPVVPPATAPSTAPVWT
                     TVEFGSYEDILKKNVYVRVGDTMAKVPASSMDAFKKKALDLRDKKVVDLDPEQVSKLV
                     LATDLPSTTQPTTKPAVNKTVILERRKITPPVLGPAAPTTQLATTQPTTGPSTMASA"
     gene            complement(1245..3377)
                     /locus_tag="VLI90_16965"
     CDS             complement(1245..3377)
                     /locus_tag="VLI90_16965"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HSV15955.1"
                     /translation="MTESNPLPRENQQQRWMKYGANVALAIVVVIVLAVVLIIISQRA
                     DRRFDMTAGKEYSLKPQTINIIKDLKSPVKLVSLYTHPENASTETTDYAGAVADLLDE
                     YKASGRNIDVETIDPVKNPTKVDDLIADVTSKYGGEVKKYRDFCDNATKYFAPIEQFS
                     KDESAKLQPLPIDRMKDDDVDATLGTIADTLAEEPARLQKIIKSVDAQRKQKVPDYKG
                     AAESIDSEMTRISDVAGQVSGAFAKLKDNPQAAPPLKQYMTDSVPRWEQVKKDADAVA
                     AEYKKLGELKLDDLRQKLRERDAILVMGPSDMRSLSFDQVWQADTDKRAYQNQKDAKI
                     KPKFAGEQQITSAILSLTTAKKPKVVFVRPGGAPLTTPGFPPFQQGGPFSKIADRLRD
                     YNFDVLEKDLSGQYAMQAQMRGEPPAPEPTDEEIKDAVWIVLSLPQQQQNPQMPPPAS
                     AGPKLAEHLKEGGSALCLFLPNSENLAEGLKDWGIDVHTDMLAVHEAVQTSSTSTDFI
                     EEAKKNPVVFITNQYGDHLITQPLKSLDSVLIPMVPIATTNKPGYQSTPILPVPQVGK
                     TWATHDVEAALNGDTVTYKPENGDLPSPLFAGSVVQSDKGNRVVAIGSLQFITNQMLS
                     IRDPELAARGIAASRFPGNADLFCNSVFWLAHMEPLIAISPAAMEVSRIGNISQGALQ
                     AWRVGLLLVGLPGAVVICGMLVYFARRD"
     gene            complement(3443..4159)
                     /locus_tag="VLI90_16970"
     CDS             complement(3443..4159)
                     /locus_tag="VLI90_16970"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSV15956.1"
                     /translation="MTRTTTIARRELSSFFFSPIAYVAMGLFLLAAGFSFWDDFQPGQ
                     PAVMRSIFERMVWLLVVVVPVLCMGLLSQEWATGTIETLMTAPIGETEVVLGKFLGSL
                     AFYLVLLVPTLLYVVLLRLYSVPDYGPIFAGYLGLFLVGALFIAIALFCSSLTRSQVV
                     AAVSSGAILLLTTIVPWYASSRATLSGFWRTAADQGVFTRYADFSKGIIDTGNLIFFI
                     VATAVFLFLTVKVLESRRWK"
     gene            complement(4325..5284)
                     /locus_tag="VLI90_16975"
     CDS             complement(4325..5284)
                     /locus_tag="VLI90_16975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSV15957.1"
                     /translation="MISVSQLTKAYGNVLAVDHISFEVPKGQVVGFLGPNGAGKSTTL
                     RMLTCYLPPTSGGATINGFDIFHQSEQVRENLGYLPENVPMYTEMRVEEYLDFRGRLR
                     KMPRELRRKRMDYVIERCWLGNVRRRVIGYLSKGYRQRVGLADSMLHNPAVLILDEPT
                     VGLDPTQIRESRKLIKDLGGDHTIMLSTHILPEVEAVCDRAIVIAGGKIVAQGTPDEL
                     RSSRRMQARVLVECRGPAKDVENALSRVSGVQAVEILNGTPDKSFVTAAIRPSDGRDV
                     REEVARTIIQSGWPLREIRLEHATLEEFFVQVTASQAMGSSLS"
     gene            5621..6307
                     /locus_tag="VLI90_16980"
     CDS             5621..6307
                     /locus_tag="VLI90_16980"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSV15958.1"
                     /translation="MSDVVSEANPNGKRPEDRLPIRILQLVDTLFARVYHDLTVISPQ
                     CLPRKGPAILICNHINSIDPVFLQASIHHRLITWMMAQEYMSLTGLGWFFRTIGIIPV
                     ARSGRDTGPLRAALRELQAGRIIGIFPEGRIAETDELLPFQTGVALMAIKTGVPVYPA
                     YIEGSQRGRDMMTAMLSRCEATVAYGPPVQFDRHSGTGRAQLEGATAAMRTAILQLRD
                     QTLAHLADRR"
     gene            6813..7310
                     /locus_tag="VLI90_16985"
     CDS             6813..7310
                     /locus_tag="VLI90_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HSV15959.1"
                     /translation="MRFLLHLLATVAWALWFGATIATFVFALHLFHTHPDIAGEANSA
                     MFVVFGTYELILAGVAIAAAGMLLVSYPSKPVVILIAWLVFTGAIGMFSALGLTPRME
                     ILRTQNKQHTDEFKRLHGRSMIAMTAQSGMLLICGAALIVTIGNRDKAPATISEDAVT
                     PAEKV"
     gene            7764..>8195
                     /locus_tag="VLI90_16990"
     CDS             7764..>8195
                     /locus_tag="VLI90_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15960.1"
                     /translation="MTNVTLSANDSAVQGGGLFNDGNAVLINVTVANNLASQGGGIYG
                     NLHPSITLALNNTTVSGNTAAKNGGGIENAGVAAQFTLANSIIAGNKVTAAGSNGPDA
                     FGPYQSRGFNLIGKKDSSTGWVSSDHTGTITAPVDAKLGALA"
BASE COUNT         1418 a         2609 c         2556 g         1612 t
ORIGIN      
        1 tcgccgacgc catcgtgctc gggccggtcg tcggctgcgt cgtcgccaac tgcgtcgtcg
       61 gcgcggccgg cccgagcacc ggcggggtta tcttccggcg ttcgaggatg accgtcttgt
      121 tcaccgcggg cttggtcgtc ggctgcgtcg tgctcggcag gtccgtcgcc agcaccagct
      181 tgctcacctg ctcgggatcg agatcgacca ccttcttgtc gcgcaggtcc agggccttct
      241 tcttgaacgc gtccatcgac gacgcgggca ccttcgccat cgtgtcgccg acgcggacgt
      301 acacgttctt cttcaggatg tcttcgtagg agccgaactc gacagtggtc caaaccggcg
      361 cggtggacgg cgccgtggcc ggcggcacca ccggggcggc ggaggtgaac gccaccgtca
      421 gctgcggctt ggccatcacg gtcgagggaa ccgtcttcgg atcgacgaag ctgtcagcgc
      481 gaaggccggt caggccgaac gtgatgtcgc tggcggcagc atcgtcggcg ggcattggcg
      541 cgggctcggt gatctgccag ttcgcgccgt tcttctgcag cgcgattttt ccgttcggcc
      601 cggtgatggt aatctgcttg atgtccgccg acgacgcggt gacgagcgtc ttcttgcgaa
      661 gatcatccag acccttggcg agcttgtcgt tcaagtcggt ggggatcatg tctgcctgcg
      721 acttgtcgcc gagcttcaca tatagattcc caccgacggc ggacgtgtcg ccgacggcga
      781 ggttgaccgt cttgctcgtc gtgatcagct gcacctgata tttcggcgta gccagcccgg
      841 tggccgacgc attcgccccg ccggcgtcca cttcgccgcg ggtgtgggcg tcggtgacgg
      901 cgcgaaccag cgcgtcgact tgccacgtct ccgccggcgc tttgaccggg tcggtgatct
      961 gccaatcggc gccgttcttc tccagcgtgt agcctggccc gtcgctcggc gtgacgacga
     1021 tcttgttgac gtccttggcg tcgatgtcga cgagcttggc gccttcggtg gaaccggagg
     1081 tgtcggtctt cgccgccggc tcgcgcgtga aataaagcgc cacccccgcc gccacgagca
     1141 acacgatcag aacgatggtc gttttgaagt tcattgattt tgtagcacgg gcgtctcgcc
     1201 cgtgtcagag catcacggca cgggcgagac gcccgtgcta cgaattaatc gcgtcgagcg
     1261 aagtacacga gcattccgca gatcaccact gccccgggca ggcccaccag cagcaggccg
     1321 acgcgccagg cttgcagcgc gccttggctg atgttgccga tgcggctgac ttccatcgcc
     1381 gcgggggaga tggcgatcag tggttccatg tgggccagcc agaagacgct gttgcagaac
     1441 agatcggcat tgcccgggaa gcgcgacgcg gcgatgccgc gggcggccag ctcggggtcg
     1501 cggatgctca gcatctggtt ggtgatgaac tgcagcgaac cgatcgccac cacgcgattt
     1561 cccttgtcgc tctgcacgac tgagccagcg aacagcggcg acggcaggtc gccgttctcc
     1621 ggcttgtacg tgaccgtgtc gccgttcagc gccgcttcca catcgtgtgt cgcccaggtc
     1681 ttgcccacct gcggcaccgg caaaatcggc gtcgattgat agccgggctt gttcgtcgtc
     1741 gcgatcggga ccattgggat cagcaccgag tccaggctct tcagcggctg cgtgatcagg
     1801 tgatcgccgt actgattggt gatgaagacg accggattct tcttcgcctc ttcgatgaaa
     1861 tcggtggatg tgcttgacgt ctgcaccgcc tcgtgcaccg cgagcatgtc ggtgtgcacg
     1921 tcgatgcccc agtccttcag tccttcggcc aggttctcgg agttgggcag gaacaggcac
     1981 aacgccgacc cgccttcctt caaatgctcc gccagcttcg gccccgccga cgccggtggc
     2041 ggcatctgcg gattctgctg ctgctgcggg aggctcagca cgatccacac cgcgtccttg
     2101 atttcttcat cggtcggctc gggcgcgggc ggttcgccgc gcatctgcgc ctgcatcgcg
     2161 tattggccgg acagatcctt ctcgagcacg tcgaagttgt agtcgcgcag tcgatccgcg
     2221 atcttcgaga acggcccgcc ctgttggaac ggcgggaaac ccggcgtcgt cagcggcgcc
     2281 ccgccgggac gaacgaacac caccttcggt ttcttcgcgg tcgtcagcga caggattgcg
     2341 ctggtgatct gctgttcacc cgcgaacttc ggcttgatct tcgcatcctt ctggttctgg
     2401 tatgcgcgct tgtcggtgtc ggcctgccag acctggtcga atgacagcga acgcatgtcg
     2461 ctggggccca tgaccaggat cgcgtcgcgc tcgcgcagct tctggcggag atcgtccagc
     2521 ttcagctcgc ccagcttctt gtactcggcc gccaccgcgt cggcgtcctt cttcacctgc
     2581 tcccagcgcg ggacgctgtc ggtcatgtac tgcttcaacg gcggcgcggc ctgcgggttg
     2641 tccttcagct tggcgaatgc gcccgacacc tgaccggcca cgtcgctgat gcgcgtcatt
     2701 tccgaatcga tcgactcggc cgcccccttg taatccggca ccttctgctt gcgctgggca
     2761 tcaaccgact tgatgatctt ctgcagccga gccggttcct cggccagcgt gtcggcgatg
     2821 gtgccgagcg tcgcgtccac gtcgtcgtcc ttcatccggt cgatcggcag cggctgcagc
     2881 ttggccgatt cgtctttgga gaactgctcg atcggcgcga aatatttcgt cgcgttgtcg
     2941 cagaaatcgc ggtacttctt cacctcgccg ccgtacttgc tggtgacgtc ggcgatcagg
     3001 tcgtccacct tcgtcgggtt cttcaccggg tcgatcgtct cgacgtcgat gttccggccg
     3061 gaggccttgt actcatcgag caagtcggcg accgcgccgg cgtaatcggt ggtctcggtg
     3121 ctcgcgttct ccgggtgcgt gtacaggctg accagcttca ccggggattt gaggtccttg
     3181 atgatgttga tcgtctgcgg cttgaggctg tattccttgc ccgccgtcat gtcgaaccgg
     3241 cggtcggcgc gctggctgat gatgatcagc acgacggcga ggacgatgac gaccacgatc
     3301 gccagggcga cgttggcgcc gtatttcatc cagcgctgct gctggttttc gcgggggagg
     3361 gggtttgatt cggtcatgtt agtagcacgg gcgtctcgcc cgtgtcagag cacttcggca
     3421 cgggctatgg cacccgtgct acttatttcc accgtcttga ttcaagcact ttgactgtca
     3481 agaagagaaa caccgccgtg gcgacgatga agaagatcag gttgccggtg tcgatgatgc
     3541 ccttgctgaa gtcggcgtaa cgcgtgaaga cgccttgatc ggcagccgtg cgccaaaagc
     3601 ccgagagcgt tgcccggctg ctggcatacc aggggacgat cgtcgtcagc agcagaatcg
     3661 ccccgctcga tacggcggcg acgacctggc ttcgcgtgag actcgagcag aacaacgcga
     3721 tcgcgatgaa tagcgcgccg acgaggaaca gcccgagata gccggcgaag atcggcccgt
     3781 agtcgggcac ggaatacagc cgcagcagca cgacgtacag cagcgtcggc accagcagca
     3841 ccaggtaaaa cgcgagcgaa ccgaggaatt ttcccagcac cacttccgtc tcgccgatcg
     3901 gcgccgtcat gagcgtttca atggtgccgg tcgcccattc ctggctgagc aatcccatgc
     3961 acagcaccgg cacgacgacc acgagcagcc agaccatgcg ctcgaagatc gaccgcatca
     4021 ccgcgggctg gccgggttga aagtcgtccc agaagctgaa gcccgcggcg agcaggaaca
     4081 agcccatcgc gacgtaggcg atcggcgaga agaagaagct ggacagctcg cggcgggcga
     4141 tggtggtggt gcgggtcatc gaatctttat cctgagcgca ggcggtactc cgccggagtc
     4201 gaaggatcta gcctcgaccg tcgatgaaac tcagcctgcg cgaatcactt caaaatctct
     4261 ctgcaaacag cggttcacat actcctcggc caggtccttc gactgcgcgg ctgcgccgct
     4321 tcgctcagga taaactagat cccatcgcct ggctcgccgt cacctgcacg aagaattctt
     4381 ccagcgtcgc gtgctccagc cggatctcgc gcagcggcca gccgctttgg atgatcgttc
     4441 gcgcgacttc ctcgcgcacg tcgcggccgt cgctgggacg aatggcggcg gtcacgaaac
     4501 tcttgtccgg cgtgccgttc aagatctcca ccgcctgcac gccgctcacg cgcgacagcg
     4561 cgttttccac atccttcgcc ggcccgcggc attcgaccag cacgcgcgcc tgcatccgcc
     4621 gactcgaacg cagttcgtcc ggcgtgcctt gcgcgacgat ctttccgccg gcgatcacga
     4681 tggcccgatc gcacaccgct tccacctcgg gaagaatgtg ggtgctcagc atgatcgtgt
     4741 ggtcgccgcc caggtctttg atcagcttgc gcgactcgcg aatctgcgtc gggtccaggc
     4801 cgacggtcgg ctcgtccaaa atcagcaccg ccgggttgtg cagcatcgaa tcggccaggc
     4861 ccacgcgctg gcgataaccc ttggacagat atccgatgac ccgccggcgg acgttgccca
     4921 gccagcagcg ttcgatcacg tagtccatgc gcttgcggcg cagctcgcgc ggcatcttgc
     4981 gaagccggcc gcggaagtcg aggtattcct cgacgcgcat ctcggtgtac atcgggacgt
     5041 tctcgggcag gtaaccgagg ttctcgcgga cctgctcgga ctggtggaaa atatcgaagc
     5101 cgttaatcgt cgccccgccg ctggtcggtg gcaggtagca ggtgagcatg cgcagcgtcg
     5161 tcgatttccc cgccccgttg ggaccgagaa acccgacgac ctgccccttg ggcacctcga
     5221 aactgatatg gtcaacggcc agcacgttgc cgtaggcctt ggtcagttga gagacggaaa
     5281 tcacgttgac gttacctcgc ttggcctcag gaaacggagg ccaccatgcc cacaccttac
     5341 cgctggggag gggacttgtt acggcgtgcc gccggtgaag tcaaggcggc gaccctcggg
     5401 ggaaaccccg ctggcagggg cgacgcccgc gtcgcccgtg atagtgttat gcgctgtcca
     5461 gccactggtt gcagcgcgat cggtaaaaac aagtcccttt ttcttgctgg catcgttcgc
     5521 ccccacgccg ggttcggagg ggactcccga cgacccggat tcttcggcgc cgataaaccc
     5581 acgttgccgc ccccgccccc gtcgctatat tcgcccccgc gtgagcgacg tggtttccga
     5641 ggcaaatccg aacggtaaac ggccggaaga ccggctgccc atccgcattc tccagctggt
     5701 cgatacgctc ttcgcccgcg tgtatcacga cctgacggtc atctcaccac aatgcctgcc
     5761 gcgcaagggg ccggccatcc tgatctgcaa tcacattaac tcgatcgatc cggtcttcct
     5821 gcaggcgtcc atccatcacc ggctgatcac ctggatgatg gcgcaggaat acatgtcgct
     5881 taccgggctg ggttggttct ttcgaacgat cggaatcatc cccgtcgccc gcagcgggcg
     5941 cgatacaggt ccccttcgcg ccgccctgcg cgaactccaa gccgggcgca tcatcggcat
     6001 ctttcccgaa ggacgaatcg ccgagacgga tgagttactg ccgttccaaa ccggcgtcgc
     6061 gctgatggcg atcaagacag gcgtgccggt ctaccctgct tacatcgaag gcagccagcg
     6121 cgggcgcgac atgatgaccg cgatgctgtc gcgctgcgag gcgaccgtcg cctatgggcc
     6181 gccggtgcag ttcgaccgcc acagcggcac cgggcgtgcc cagctcgaag gcgccaccgc
     6241 cgcgatgcga acggcgatct tacagctgcg cgaccagacg cttgcccacc ttgccgaccg
     6301 tcgctaaaca cttgccaaag ctgcaggaat acagcacacc atagttttgc ataaaaatga
     6361 gcgcgctcgc gccagtggtt ggaattgtta cagtttcatc gagccatccg ccgatgggtt
     6421 ttgcgtaagg aagagccgct tttaaaagat tgcgtttgac ggattggaag cggacggtac
     6481 tataatttag gcaaattgaa acgatcgcgc cgagtgcgca gtcaacggcg cattttctcc
     6541 cctccggaag acaccgtgca gaaatgccgg tgtcttcttt atttgcaatt catgttactc
     6601 tcatccggtg atctcccgtt cgcgccaaga cgccaagacg tcaagagcgc caagggtaaa
     6661 gaactaaacc cgaactacgc gactcgcctg atgctgaaat ctgtctgaca ttcttagcac
     6721 ggctctgcgt ttagcgtttc ttccccttgg cgctcttggc gcgacagcaa gcctgcgtgc
     6781 cacgttgact gacggcccat atactcgcgt cgatgcgatt cctgcttcac ttgctcgcga
     6841 ccgtcgcctg ggccctctgg ttcggcgcga cgatcgccac cttcgtcttc gcgctgcacc
     6901 tgttccacac gcacccggac attgccgggg aagccaacag cgcgatgttc gtcgtgtttg
     6961 gaacgtatga attaattttg gccggcgtgg cgatcgcggc ggcggggatg ctgctggtga
     7021 gttacccgag caagccggtg gtgatcctga tcgcctggct ggtcttcacc ggcgcgatcg
     7081 gcatgttcag cgcgctgggc ctgacgccgc ggatggaaat cctgcgcacg cagaacaaac
     7141 agcacaccga tgagttcaaa cgcctgcacg ggcgatcgat gatcgcgatg acggcgcaat
     7201 ccgggatgct gctgatctgc ggggcagcgc tgatcgtgac gatcggcaat cgcgacaaag
     7261 cgccggcgac gatctccgaa gacgcggtga ctcccgccga aaaggtatga ccaaccccac
     7321 gccgggttcg gaggtactcc gacgaagcaa gatgctttct cgcgcgcagg aggatccggg
     7381 ttgtcgccag tacgttccga acccggcgcg gggacgagtc tgcgcgcttg atgcgaagca
     7441 cgccaatggt catattcttg ctctggccgc accccgtcgg cggggaatcg cggcgggctt
     7501 gggggtcggc acatcagagg aggtcaaatc atgcagattc gaacgatccg gtcctgcgcg
     7561 cgccgcgcgc gtcgcgctgt catcggggcc acggttgaag caatggagca tcgcctgctt
     7621 ttgaccgccg tggcaccggc gattgggggg cgggcatttc gaatgccggc attctcaccc
     7681 tgaccaacgc cactgtcgcc ggcaacaacg ccagctatgt cggtggtggg atctacaatt
     7741 cgctgtatgc gggaaagctg acgatgacca acgtcacgtt gtcggccaac gattccgctg
     7801 tacagggcgg tggcttgttc aacgacggca acgccgtgct gatcaacgtc accgtggcaa
     7861 ataatttggc gagccagggc ggtgggattt acgggaactt gcatccgtcg atcacgttgg
     7921 cgctgaacaa cacgaccgtc tccggcaaca ccgcggccaa gaatggcggt gggatcgaga
     7981 acgccggcgt ggccgctcag ttcacgctgg ccaacagcat catcgcgggc aacaaggtga
     8041 cggcggcggg cagtaacggg ccggatgcgt tcgggccata tcagtcgcgc gggttcaatc
     8101 tcatcgggaa gaaggacagc agcaccggtt gggtttcgtc cgatcacacc ggcacgatta
     8161 ccgcgccggt ggatgcaaag ctgggcgcgc tggcc
//
LOCUS       DATIMH010000979         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1681026, whole genome shotgun sequence.
ACCESSION   DATIMH010000979 DATIMH010000000
VERSION     DATIMH010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681026"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..972
                     /locus_tag="VLI90_16995"
     CDS             361..972
                     /locus_tag="VLI90_16995"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF004685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HSV15961.1"
                     /translation="MQIVTAITGASGALYAQRFIQGLCAANVGVHLVVSPLGRRLLHD
                     ELGMETVDLPALAGAEQHTITLYNYNDVGSKLASGSFLHDGMVIVPCSSNTLAEIAHG
                     IGDNLISRAAAVALKERRRLILAHREMPLSPIDVNNYKTLTDAGAILAPANPGFYLNP
                     ATVAEVVDFVAGKLLDLIGVPHAMDTRWDPKQVRPPAKREPAI"
     gene            1115..1477
                     /locus_tag="VLI90_17000"
     CDS             1115..1477
                     /locus_tag="VLI90_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15962.1"
                     /translation="MTVISRDVDQWILIGQDIRVSPTDIDAQGVRLVARGRVLGGPDD
                     GATFDIRHELAIGQTWHLGPHVVVSLVEVRGDSARLGVLAPAHIDVQTKEHLDHQRGT
                     GGGGTGGSGGGERGSHGK"
     gene            1467..2438
                     /locus_tag="VLI90_17005"
     CDS             1467..2438
                     /locus_tag="VLI90_17005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HSV15963.1"
                     /translation="MASETLPTPADPFKPAVSLVEPRVGPAIIQNVRTLADDIKLHHS
                     VFALPWAILATVLAAHRTAGGLRLGQFLLILICMVTARTLAMAANRLLDAEVDAKNPR
                     TARRAVPSGRLSRGFMIATIAACAVIFIAATAGFWFWYRNPWPLLLATPVLLFLSSYP
                     YLKRFTRLCHYYLGAALALAPICAWLAIKGTLNAPPLYMAGAVLLWTAGFDIIYACQD
                     YAFDVEHGLFSVPAKLGIARALWVARLTHLACVAFLITLGLRTPELSTLYWLGVTAAV
                     TLLVIEHALVKPNDLSKVGLAFFTVNGIISVLLGSLGVADVLVHHVR"
BASE COUNT          420 a          871 c          823 g          492 t
ORIGIN      
        1 gttcggcatc ctccagcgca ggcgtactcg ccggagtcga acgatcttgc cggcgttggc
       61 gaacggccac tcgcgcaaat cagccgcttg cggcttcgca attcagcggc tcctgatctg
      121 cgaagccgca agcggctgat ttgcgcgagg ggcggttcac caacacgccg gcaagatcct
      181 tcgacgccgg acttcggcga gctcagtcga ggcgcgagta cgcgtgcgct ggaggacgac
      241 gatcgtctct taacatcgtc tcggcgggct cggcgccctc ggcggttgca tttgacctgc
      301 tttcaaactt tgcgcgcacg cgcggcgcgg cgactgttgc tcgatataat gcgtcaccac
      361 atgcagatcg tcaccgccat caccggcgcc agcggcgcgc tgtacgctca gcgattcatc
      421 cagggtttgt gcgctgccaa cgtcggcgtg cacctggtcg tctcgccgct gggccggcgg
      481 ctgttgcacg atgaactggg gatggagacg gtcgatctgc cggcgctggc tggcgccgag
      541 cagcacacga tcacgcttta caactacaac gacgtcggct cgaaacttgc cagcggcagc
      601 ttcctgcacg atggcatggt catcgtcccc tgctcgagca acacgctggc ggagatcgcg
      661 catggcatcg gggataacct gatctcgcgc gcggctgccg tcgcgctgaa agaacgtcgc
      721 cggctcatcc tcgcccaccg cgagatgccg ctgtcaccga tcgacgtgaa caattacaaa
      781 acgctcaccg acgcgggcgc gattctcgcc ccggcgaatc ccggttttta tttgaacccg
      841 gcgaccgtcg ccgaagtagt ggactttgtt gcgggcaagt tattggactt gatcggcgtg
      901 ccgcacgcga tggacacgcg gtgggacccc aagcaggtcc gcccgccggc caaacgcgag
      961 ccggcgattt gatttctgca acgcgtctgc cggacgtttg acgaaaggca gttggtagga
     1021 caagctattg tcatcccccg cggaggggcg actgctgttg ccgccacacg cgcacgggtt
     1081 gttgggcttt gtgggctgga cggggagcgt ttgcatgacg gtcatttcac gtgacgtcga
     1141 tcagtggatc ctgatcgggc aggacatacg cgtgtcgcca accgacatcg atgcccaggg
     1201 ggtgcgactg gtcgcgcgcg gccgggtgct cggtgggcct gacgacggcg caacattcga
     1261 catccgccac gagctggcga tcggtcagac ctggcacctc gggccgcacg ttgtggtgag
     1321 cctggttgaa gtccgcggcg acagcgcccg gcttggcgtg ctggcgccgg cgcacatcga
     1381 cgtgcagacc aaggagcacc tggaccacca gcgcggcacc ggtggtggtg gcactggcgg
     1441 cagcggcggc ggtgaacgcg gcagtcatgg caagtgagac gttgcccaca ccggccgacc
     1501 cctttaagcc tgcggtgagc ctggtcgaac cgcgcgtcgg gcctgcgatc atccaaaacg
     1561 ttcgcacgct cgcggacgat atcaaacttc atcacagcgt cttcgcgctg ccgtgggcga
     1621 tcctcgccac agtgcttgcc gcccatcgca ccgcaggcgg attacggctc ggccaattcc
     1681 tgctgatcct gatctgcatg gtgaccgcgc gcacgctggc gatggcggcc aatcgcctgc
     1741 tggacgccga ggttgatgcg aagaacccgc gcaccgcgcg gcgtgcggtg cccagcggcc
     1801 gactgtcgcg cggtttcatg atcgcgacga tcgccgcgtg cgccgtgatc ttcatcgccg
     1861 ccaccgcggg tttctggttc tggtatcgca atccttggcc gctgctgctg gccacgccgg
     1921 tgctgctgtt cctgagcagc tatccgtacc tgaagcgctt cacgcgcttg tgccactact
     1981 atctcggcgc cgcgctggca ctcgcgccga tctgcgcctg gctcgcgatc aaaggcacgc
     2041 tcaacgcccc gccgctctac atggccggtg ccgtgctgct gtggaccgcc ggcttcgaca
     2101 tcatttacgc ttgccaggat tacgctttcg acgtggagca cgggctgttc tccgtgccgg
     2161 cgaaactcgg catcgcccgc gccctctggg tcgcccgcct cacgcacctc gcgtgcgtcg
     2221 cgttcctcat cacgctcggc ctgcgaacgc ccgagctgtc cacgctctat tggctcggcg
     2281 tcaccgccgc cgtcaccctg ctcgttatcg agcacgcgct ggtgaagccc aacgacttgt
     2341 ccaaagtcgg cctcgcgttc tttaccgtca atggcatcat cagcgtgcta ctgggctccc
     2401 tcggcgtggc agatgtgctg gtgcatcacg tcaggtgatt cgatcaatcg aggcgctcct
     2461 cgtcgaacaa ttgaagaaac gcggtgaccg ccggcgactt gctccgcccg cgccgccgga
     2521 cgtggtagat cgggtatcga atcgccatcg ccttcacggg gacctcgacg atctgccgcg
     2581 ctcgaacctc catccgaatc gacaac
//
LOCUS       DATIMH010000980         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3983652, whole genome shotgun sequence.
ACCESSION   DATIMH010000980 DATIMH010000000
VERSION     DATIMH010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3983652"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VLI90_17010"
     CDS             <1..259
                     /locus_tag="VLI90_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15964.1"
                     /translation="PRWRATAVFRHEPTVPAAIPVREMRVLYGLKDHHYAGFARHQLP
                     HAHEFFVPMKTLTEENSKDNPRLRALLERASVSSNFYNQAD"
     gene            complement(247..1098)
                     /locus_tag="VLI90_17015"
     CDS             complement(247..1098)
                     /locus_tag="VLI90_17015"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HSV15965.1"
                     /translation="MARRVAVIGHRGARGLFPENTLEGFRRAFDLGVRAFELDVGMTA
                     DGVVVVSHDLALNPAITRDANGRFLTEPTPAIHDLTLDALGAYDVGRIRPASKYRLPH
                     RAQTPIDGARVPTLDAVLRAQPEAEFIIELKTDPRYPERTVSPVALADAVLAIVDAAG
                     AAGRIILESFDWRGPRHIHRVRPELKLAWLTREETVRDAKLWWDGAVPNPSVPAAVAA
                     EGGGVWAPEFTTLTPASLAEARDLGLRVIPWTVNRLGAMRRLMRWGVDGIITDRPDKL
                     LREIQSA"
     gene            1147..>2037
                     /locus_tag="VLI90_17020"
     CDS             1147..>2037
                     /locus_tag="VLI90_17020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV15966.1"
                     /translation="MDYTTLGRTGLRVSVAGLGTGGFSRVGIGKSEDHAVGIIRQALD
                     LGVNLIDTAAVYGTEGVVGRALKDVKRDSVVVCTKASKPVNDPAFTVKQILESLDGSL
                     SRLGMDYVDVFQLHAVPPAAYEYVRNEIVPALLREKEKGKFRFLGITETSPNDHEMVM
                     MQKAAATDDTPWDTAMFAFNLMDQVAREKVFPLTLKHRVGTLMMFAVRSIFARPAQLA
                     DTMKDLAAKGLVPPDLAANDSPLGFLVHADGASSVTDAAYRYVRHEPGVDVVLFGTGD
                     PEHLKSNIASILKPPLPAADQ"
BASE COUNT          362 a          659 c          678 g          338 t
ORIGIN      
        1 gccgcgctgg cgggcgaccg ccgtgttccg gcatgaaccc acggtgccgg cggcgatccc
       61 ggtgcgcgag atgcgcgtgc tgtacggcct caaggaccac cattacgccg gcttcgcgcg
      121 acatcagtta ccgcatgcgc acgagttctt cgtgccgatg aagacgctga ccgaggagaa
      181 cagcaaggac aaccccagat tgcgggcgtt gctggagagg gcgtcggtgt cctcgaactt
      241 ctataatcag gccgactgaa tctctcgcag cagcttgtca ggccggtcgg tgatgatgcc
      301 gtcgacaccc cagcgcatca aacgccgcat cgcgccaagg cggttgacgg tccaggggat
      361 cacgcgcagc cccagatcac gggcttcggc cagcgacgcg ggcgtcaggg tggtgaattc
      421 gggagcccag acccctcccc cttcggcggc gaccgccgcg ggaaccgagg ggttcggaac
      481 ggccccgtcc caccacagct tcgcgtcgcg caccgtctct tcccgcgtga gccaggcgag
      541 tttcagttcg gggcggacgc gatggatgtg ccgggggccg cgccagtcga acgattccag
      601 aatgatccgt ccggcggcgc ccgccgcgtc gacgatcgcc agcacggcgt cggccagcgc
      661 taccggcgat accgtccgct cggggtaacg cgggtcggtc ttcaattcga tgatgaactc
      721 cgcctccggt tgggcgcgca gcaccgcgtc cagggtcggc acgcgggcgc catcgatcgg
      781 cgtttgcgcc cggtgcggca ggcgatactt cgacgccgga cggatgcggc ccacgtcgta
      841 cgcgcccaaa gcatcgagag tcaggtcgtg aatcgccggg gtcggttccg tcagaaaccg
      901 tccgttcgcg tcgcgggtga tcgcgggatt cagcgcgaga tcgtggctga ccaccacgac
      961 accgtccgcc gtcatgccaa cgtcgagttc gaacgcgcgc acgcccaggt cgaaagcgcg
     1021 gcgaaaaccc tccagggtat tttccggaaa cagcccgcgc gcgccgcgat ggccaatgac
     1081 cgcaacccgt cgcgccatgc aaatgtccct gttagactcc gccgaatcgt cacggggagt
     1141 tcgatcatgg attataccac acttggccgc accggcctgc gggtcagtgt cgccggtctc
     1201 ggcaccggcg ggttcagccg cgtcggcatc ggcaaatccg aggatcacgc ggtcggaatc
     1261 atccgtcagg cgctcgatct cggggtcaat ctgatcgaca cggcggcggt ttatgggact
     1321 gagggcgtgg tcggacgggc gctgaaggac gtgaagcgcg acagcgtggt cgtatgcacc
     1381 aaggcgtcga agccggtgaa cgacccggcg ttcacggtga agcagatcct ggagagtctc
     1441 gacggctctc tgagccgcct cggcatggat tatgtcgatg tgttccagtt gcacgcggta
     1501 ccgcccgccg cctacgagta cgtgcggaat gagatcgttc cggccctgtt gcgggaaaag
     1561 gaaaagggca aattccgctt tctgggcatc accgagacgt ccccgaacga tcatgaaatg
     1621 gtgatgatgc aaaaggcggc cgcgaccgat gacacgccct gggacaccgc gatgttcgcc
     1681 ttcaacctga tggaccaggt ggcgcgggag aaagtcttcc ctttaaccct gaagcaccgc
     1741 gtcggcacgc tgatgatgtt cgccgtgcgc agcatcttcg cccgcccggc gcaactggcg
     1801 gatacgatga aagacctcgc ggcgaagggc ctggtgcccc cggacctggc ggcgaacgac
     1861 tcgccattgg gctttctggt ccatgcggac ggcgcgagtt ccgtgaccga cgccgcgtat
     1921 cgttacgtgc ggcatgagcc gggcgtggat gtcgtgctgt tcggcaccgg ggaccccgag
     1981 catctgaagt ccaacatcgc ttccattctg aaaccgccgc tgccggcggc ggaccag
//
LOCUS       DATIMH010000981         4802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_524496, whole genome shotgun sequence.
ACCESSION   DATIMH010000981 DATIMH010000000
VERSION     DATIMH010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524496"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1764)
                     /locus_tag="VLI90_17025"
     CDS             complement(1..1764)
                     /locus_tag="VLI90_17025"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV15967.1"
                     /translation="MTAQEMVQQGLAHHQAGRRAEAEALYRRALQRDPGNPAVLALLG
                     SLLMDTGDGGATEAVELCTKATRLAPNVAQFHHALGTALANLRRFPEAIGAFRRAIQC
                     HRDYADGHAGLGRALASVGQLDEAIASFRTAMTLKPNFAPAYVDLASALRRAGKLDEA
                     VNVATRATEFDPRSGKAHNILGLIYADRHDDARAIQHLERALELQPDLADAHLNIAQV
                     LMQQRNDLDAAIAACRRAIALRPTYAEAHANLGILLVENAQIDEAMAEYRRAIELNPD
                     SNDAHWNYGLALLLSGDFDRGWIEFDRGRLKAANDLRLLKTRPEWTGEDLHGKTILLY
                     GEQGLGDTLQFVRYAPLVAERGGRVILGCQRELARLLTNFPGVDEIVPGDAPVPPFDL
                     HCSLLSLPRIFKTTADSISGGMGYLEADPSLADAWAQRLGERAPGVRRIGLVWAGNPQ
                     HKNDRNRSTKLEQLAGLAALPRVQIYSLQKGPAAAQLRDMPQLNIIDHTAELNDFADT
                     AALISQLDMVITVDTSVAHLSGALGVETWLMLPVAPDWRWMLNRDDSPWYPTMRLYRQ
                     QKRRDWTSVAEQVLRRITVMQ"
     gene            complement(1761..2198)
                     /locus_tag="VLI90_17030"
     CDS             complement(1761..2198)
                     /locus_tag="VLI90_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HSV15968.1"
                     /translation="MNLIKLLIVCLALTFFPSAFAAGEQPTTAPATEPSAGVISAGDK
                     QALQDHLDKEVTVEGVVSDAKWSSSGKVFIIKFKEAGDSQFQAATFSKNKDAMEKAFD
                     GDLTKAFEGAKIQVKGRLQTFKEHPEILVDKPEQITVTEKAGG"
     gene            complement(2308..3129)
                     /locus_tag="VLI90_17035"
     CDS             complement(2308..3129)
                     /locus_tag="VLI90_17035"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HSV15969.1"
                     /translation="MSDRGFDFLRVNARPSKPRTRGMTEIRGPYYTPMGKRYLADVLE
                     TMGEYIDSLKFAGGAFTLMPRAAVKELIDLAHEHNVACSTGGFIETVIPQGPAAVDSY
                     LRECKALGFDVVEVSSGFITIPLDDWVRLVQQVQCTGMKAKAEVGIQFGAGGASDVAA
                     LEAIGTADAGWMIQRAQRLLDAGAFMVMIESEGITECVKTWRTEVAGRVVESLGLERV
                     MFEAADPAVFARYIQNYGPEVNLFVDHSQIVQLECLRSGIWGTNDLWGRVHTYKA"
     gene            complement(3157..3654)
                     /locus_tag="VLI90_17040"
     CDS             complement(3157..3654)
                     /locus_tag="VLI90_17040"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSV15970.1"
                     /translation="MIASLLAQRAALAQQLPIAPGPRRVIIQAMVQIDIEYQGSLRTQ
                     ARHEPSGSTFLTDAPKDNQGNGEAFSPTDLLATALGTCMVTTMGIAAKRVNVDLVGTT
                     VRVLKEMIATPTRRLAQLTVEIHCPASAPPEHRPLLERAALNCPVVKSLHPDVQIPAQ
                     ILWDR"
     gene            4260..>4802
                     /locus_tag="VLI90_17045"
     CDS             4260..>4802
                     /locus_tag="VLI90_17045"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15971.1"
                     /translation="MRKQHSDSARAGQHRQSSSKRSTNKRRRAGATISRSTHAILERL
                     EDRQLLSTSVSFSNGVLTLNGDPKLANHLTVEQDPTGKNLGANAQGKLLSVSKGSVKQ
                     IVINSGSGADRIYIDNTIAIPTTINSGDGNDIIKAGAGNATVTAGSGRDWIAVHGAHT
                     SVKIGNGDSTVLGGIGDDTIV"
BASE COUNT          816 a         1459 c         1598 g          929 t
ORIGIN      
        1 tcactgcatc actgtgattc gccggaggac ttgctcggcg acgctggtcc agtcgcggcg
       61 cttttgctgg cggtacaggc gcatcgtcgg ataccagggg ctgtcgtcgc ggttaagcat
      121 ccatcgccag tcgggggcga cgggcagcat cagccaggtt tcgacgccga gcgcgccgct
      181 gaggtgggcg acggatgtat cgaccgtgat gaccatgtcc agctgtgaga tcaacgcggc
      241 ggtgtcggcg aagtcattca gctctgcggt gtggtcgatg atgttcagct gcggcatgtc
      301 gcgcagctgt gcggcggcgg ggcctttttg cagcgagtaa atctgcacgc gcggcagcgc
      361 ggcgaggccc gccagttgct cgagcttcgt cgagcgattg cggtcgttct tgtgctgcgg
      421 attgccggcc cagacgaggc cgatgcggcg gacgccggga gcgcgctcgc caagtcgctg
      481 ggcccacgcg tcggcgagcg acgggtcggc ttctagatac cccatgccgc cggagattga
      541 gtcggcggtg gttttgaaaa tgcgcggaag gctgagcagc gagcaatgca gatcgaacgg
      601 cgggactggt gcatcgccgg ggacgatttc gtctacgccg ggaaagttgg tgaggaggcg
      661 ggcgagttcg cgttggcagc cgaggatgac gcggccgccg cgctcggcga cgagcggggc
      721 gtagcggacg aactgcagcg tatcgccgag gccctgctcg ccgtagagca ggatcgtctt
      781 gccgtggagg tcttcgccgg tccattcggg gcgggtcttg agaaggcgca ggtcgttggc
      841 cgccttcagg cggccgcggt cgaactcgat ccaaccgcgg tcgaagtcgc cgctgagcag
      901 cagggcgagc ccatagttcc agtgggcgtc gttcgagtcg ggattgagct cgatcgcgcg
      961 acgatattcg gccatcgctt catcgatctg ggcattctca accagcagga tgccgaggtt
     1021 cgcgtgcgct tcggcgtagg tgggtcgaag cgcaatcgcc cggcgacacg cagcaatggc
     1081 cgcatcgagg tcgttccgct gctgcatcaa cacctgcgcg atgttcaggt gcgcgtcggc
     1141 caggtcaggt tgcaactcga gcgcgcgctc gaggtgctga atcgcgcggg cgtcgtcgtg
     1201 gcgatcggcg tagatcaagc cgaggatgtt gtgcgccttg cccgagcgcg gatcgaactc
     1261 cgtcgcgcgc gtcgcaacat tgaccgcttc atcgagtttg ccggctcgtc gaagggcgct
     1321 ggcgagatcg acgtacgccg gcgcgaagtt cggcttcaac gtcatcgctg ttcggaagct
     1381 ggcgatcgct tcatcgagtt gtccaacgct ggcgagcgcg cggcccaggc ccgcgtgacc
     1441 atcggcgtaa tcgcggtggc attggatggc tcgacggaac gcgccgatgg cttcgggaaa
     1501 tcgccgcagg ttggccagcg cggtgccgag tgcgtggtgg aactgcgcca cgttcggcgc
     1561 cagccgggtg gccttggtgc aaagctcgac cgcttcggtc gccccgccgt cgccggtgtc
     1621 catcagcagg gaaccgagca gcgctaagac ggcggggttg cccgggtcgc gctgcagcgc
     1681 gcggcgatac agcgcctcgg cctcggcgcg ccggcccgcc tggtggtggg ccagcccctg
     1741 ttgcaccatt tcctgcgcgg tcatccgccg gccttttcgg tcacggtgat ctgttccggt
     1801 ttgtccacga ggatctcggg atgttccttg aacgtctgca ggcggccttt gacttggatc
     1861 ttcgcccctt cgaacgcctt ggtcaggtcg ccatcgaacg ccttctccat cgcgtccttg
     1921 ttcttggaga aggtggcggc ttggaactgg ctgtcgccgg cctccttgaa cttgatgatg
     1981 aagaccttgc cgctgctgct ccatttggcg tcggagacga cgccttcgac agtcacttcc
     2041 ttgtccaagt ggtcttgcag cgcctgcttg tcaccagcgc tgatgacgcc ggcggacggc
     2101 tcggtcgccg gagcggtcgt cggttgttcg ccagccgcga aggcgctcgg aaagaaagtg
     2161 agcgcgaggc agacaatcaa caatttgatc aggttcatgg atctccttct cttgtggttc
     2221 ggattgtact ggaagacgcg cggatcagcg taattgatct gcgaagccgc aagcgacttc
     2281 gcgggctcgt cggcggacat ggccagttta agctttatag gtgtgcaccc gtccccatag
     2341 atcattggtg ccccagatgc cgctgcgcag gcattccagc tgcacgatct ggctgtgatc
     2401 gacgaagagg ttcacctccg gcccgtagtt ctggatgtac cgcgcgaaga ccgcggggtc
     2461 ggcggcttcg aacatcacgc gctcgaggcc aagcgattcg accacccgcc cggcgacttc
     2521 cgtccgccag gtcttcacgc attcggtgat cccttcggat tcaatcatga ccatgaacgc
     2581 gccggcgtcg agcaggcgtt gggcgcgctg gatcatccac cccgcgtcgg cggtgccgat
     2641 cgcttcgagg gcggccacgt cgctggcgcc gccggcgccg aattggatgc cgacttccgc
     2701 cttggctttc atgccggtgc attgcacctg ttgcacgaga cgcacccagt cgtcgagcgg
     2761 gatcgtgatg aagccggagg agacttcgac gacgtcgaag ccgagcgctt tgcattcgcg
     2821 caggtatgaa tcgacggcgg cggggccttg ggggatgacg gtttcgatga agccgccggt
     2881 ggagcaggcg acgttgtgtt cgtgcgcgag gtcgatcaat tccttgacgg ccgctcgcgg
     2941 catcagcgtg aacgcgccgc cggcgaactt cagggagtcg atgtactcac ccatcgtctc
     3001 caggacgtcg gcgaggtagc gcttgcccat cggggtgtag tagggcccgc ggatttcggt
     3061 catgccgcgc gtgcgcggct tggatggccg ggcgttgacg cgcagaaagt cgaaaccgcg
     3121 atcgctcatt tctccgtcat ttcagccgtg cgacgttcag cgatcccaca aaatctgcgc
     3181 gggaatctga acatccggat gcagactttt gacaaccggg cagttcagcg cggcgcgttc
     3241 cagcagcggc cggtgctcgg gcggcgcgct ggcggggcag tggatttcga ccgtcagctg
     3301 ggcgaggcgg cgcgtcggcg tcgcaatcat ttccttcagc acgcgcaccg tcgttcccac
     3361 cagatcgacg ttcacgcgct tcgcggcgat gcccatcgtc gtcaccatgc aggtgcccag
     3421 cgccgtggcg agcagatccg ttgggctgaa ggcttcaccg ttgccctggt tgtccttggg
     3481 cgcatcggtg aggaacgtgg agcctgacgg ctcgtgccgg gcctgggtgc ggaggcttcc
     3541 ctgatactcg atgtcgatct gaaccatggc ctggatgata acgcgccgcg gccccggcgc
     3601 gatcggcagt tgctgcgcga gcgctgcgcg ttgcgcgagc agggatgcaa tcaattgtga
     3661 ttaatatcaa cgcggtgtga tcgtcaaaaa aaccccgaaa aatctgagaa aatggtgaac
     3721 cttgcgcgtc tggtgagcgt tgccgtcgct cattggtgtg ctcgcagcga ctttttttta
     3781 tttttacccg gtttaccgct tgaacgattt cgctcgttcc atactttcgt ccgcaagaca
     3841 tcgaggggcg aaaccgcggg ctcggtttcg caaggctgtg ccctctgaga tgtcgcagcg
     3901 cgccgtggaa tttccaacca gaggaacgaa ccaatgagac gacggcaacg caatgccggt
     3961 ctgcgtgttc actgcgtctc cctgattgtg aacaccaccc cgcgcggggg ccgcaagatc
     4021 gaacgacttc taccccgcat ggcatctcgc acactgagcc ctgtccctct gcccgccgtg
     4081 ggtggcttgt cggttctttg agtttctcgc gaacctgcaa cgccagaacg ctcctgcagc
     4141 agctcgctgc tgagctgctc ttaagccgcg ggtcaaccga cgcaacgtcg cgttgaccaa
     4201 ccagccgggc tatcgggcaa gaaaagtttg tgaatttttt cttgattggg acgaacatca
     4261 tgcgaaagca acactctgat tccgcgcggg cgggtcaaca tcggcaatct tccagcaagc
     4321 gttcgacgaa caagcggcgt cgtgccggcg cgacgatctc gcgcagcaca cacgcgatcc
     4381 tcgagcgcct cgaggaccgc cagctgctgt ccacctcggt cagcttcagc aacggtgtgc
     4441 tgacgctcaa cggtgatccg aagctggcta accacctgac ggtcgagcag gacccgaccg
     4501 gcaagaacct cggcgccaac gcccagggca agctgctcag cgtcagcaaa ggttccgtga
     4561 agcagatcgt catcaacagc ggcagcggcg ctgatcgcat ctacatcgac aacacgatcg
     4621 cgatcccgac gaccatcaac agcggcgacg ggaacgacat catcaaagcc ggcgcgggca
     4681 acgcgaccgt cacggcggga agtgggcgcg actggatcgc cgtgcatggt gcgcacacgt
     4741 cggtcaaaat tggcaatggc gacagcaccg tgctcggcgg catcggtgac gacacgatcg
     4801 tc
//
LOCUS       DATIMH010000982         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1682034, whole genome shotgun sequence.
ACCESSION   DATIMH010000982 DATIMH010000000
VERSION     DATIMH010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682034"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..>2837
                     /locus_tag="VLI90_17050"
     CDS             229..>2837
                     /locus_tag="VLI90_17050"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HSV15972.1"
                     /translation="MPRRPCALRLWGIVLFILGLGASAAQATPPVLLSAVSRMTHSTA
                     GTFDVQLPLSGGTGVECRTVASGLTIVLTFDQAVVSGSAAVVPAAASVNGAPTFAGNT
                     MTVKLNVTADAQTITLTLSNVANGASELLPSQAVTFRTLLGDVNANGLLSGSDVNIAK
                     AAVAANVGVTSGNFRSDVNANGSLSGTDVNMIKAAVSAGTTVGGGATANTPPTISTIA
                     NQQAVTGQPMSPVGFTVGDAESDPSTIYVSATSSDLTTIPNANITFTGTGTSRTINIT
                     PASGVVANTQVVITMTASDGLALSSPSTFTVTVTPPPGVYVATLQPIAGTNSLGSGTA
                     TLTLSGDQTYAILKYSLSNLAGTDTDDTVDAPGDQILYDVPVGRSHGDLQPDGSLKWI
                     FNVNQAASIVQTITGNNAYMVINTSANPAGELKGTFQKVVGSQTFTPPPPPPSITINP
                     PTAADASRFLQQSQFGGTGTEIASLSNSGAANASTAINDWLNAQFAQPGPIYPDYSNA
                     AVAPTTQPVTPPGPQSASQPYTSSSMYYNMYVRCCSPQSPNPYGDNLDDTLIHETWWK
                     NTVTAPDQLRQRVATAYSEIFVVSEINDNLDAQIPGMCTYYDMLADDAFVSFKQLLSD
                     ITLHPIMGEYLNMRGNKKATAPAVPNENYGREVLQLFSIGLYMLQPDGTLMLDANGQP
                     IPTYDQPTITSFAQVFTGWDVNNTGVTVPELISDGAGGATTANFTSRYQKPMVLTQSN
                     HSTTVKNLLVYPGAATWSFGGASGPSQIPANSGQTASVELNFAIDNIVNHPNVGPFIC
                     RQLIQRLVGSNPSPAYVYRVAQVFNNDGSGNRGNMKAVITAILTDYEARSPAVLSDPG
                     YGHMREPMIRVASL"
BASE COUNT          548 a          926 c          881 g          482 t
ORIGIN      
        1 ggcgcgaatc gaaaatcatg ctcaatattg cccggaaatc ttttgacagc gttaccagaa
       61 actaagtacc attcgatcgc tcaggaggag aatctgcgtt gcttctgccg cggtggcggc
      121 ggacttggct cccttagatc ggtcgtccaa cagccttttt ctgcgcgtct ttcttgttct
      181 cgcgtgcctt tttcggacaa ctcaggcgga cttggtttgg aggattcaat gcctcgccgt
      241 ccttgcgctt tgcggctctg gggaatcgtg ctgttcatcc ttgggctcgg cgccagcgct
      301 gcgcaggcga cgccgccggt gctgttgtcg gcggtctcgc gcatgacgca cagcaccgcg
      361 ggcacgttcg acgtgcagct gccgctgagc ggtggcaccg gcgtcgaatg ccgaaccgtc
      421 gcgagcgggc tgacgatcgt cctcaccttc gatcaagcgg tggtcagcgg ctcggccgcg
      481 gtcgtgccgg cggcagccag cgtcaacggc gcgccgacgt tcgccggcaa cacgatgacg
      541 gtgaagctga acgtcaccgc cgacgcgcag acgatcacgc tcacgctatc caacgtcgcc
      601 aatggagcca gcgaactgct gccgtcgcag gccgtgacct ttcgcacgct gctgggcgac
      661 gtgaacgcga acgggttgtt gtccggatct gacgtgaaca tcgccaaggc cgccgtcgct
      721 gccaatgttg gtgtcaccag cgggaacttt cgctcggacg tcaacgccaa cggcagcctg
      781 agcggcaccg acgtgaacat gatcaaggcg gccgtttccg ccgggacgac cgtgggcggc
      841 ggggcgacgg cgaacacgcc gccgacgatc agcacgatcg ccaaccaaca ggcggtgacg
      901 ggccaaccga tgtcgccggt gggattcacc gttggcgatg ccgagtcgga cccttccacg
      961 atctacgtca gcgccacctc gagcgatctg acgacgatcc ccaacgccaa catcacgttc
     1021 accgggacgg gcacgtcgcg cacgatcaac atcacgccgg cttcgggtgt ggtggcgaat
     1081 acgcaggtgg tgatcacgat gacggcgtcg gatggattgg cgttgtcgtc gccgtccacg
     1141 ttcaccgtga cggtgacccc gccgccgggc gtttatgtgg cgacgctgca accgatcgcc
     1201 ggcaccaact cgctcggcag cggcaccgcg acgctgacgc tgagcggcga tcaaacttac
     1261 gccatcctga aatatagcct gtcgaacctg gccgggacgg acaccgacga cactgttgat
     1321 gcgccgggcg atcagatcct gtacgacgtg ccggtcgggc gttcgcatgg cgatcttcag
     1381 ccggacggtt cgctcaagtg gatcttcaac gtgaatcaag ccgcgtcgat cgtgcagacg
     1441 atcacgggca acaacgcgta catggtgatc aacacctcgg ccaacccggc gggggagttg
     1501 aaggggacgt tccagaaggt ggtgggatcg cagacgttca caccgccacc gccgccgccg
     1561 tcgatcacga tcaatccacc aacagcggcg gatgcgtcgc ggttcctgca gcagtcgcag
     1621 ttcggcggga cgggcacgga gatcgccagc ctgtccaaca gcggcgcggc caacgcgagc
     1681 accgcgatta acgattggct gaacgcccaa ttcgcccagc cggggccgat ctatccggat
     1741 tattccaacg ccgccgtcgc gccgaccacg cagccggtca ccccgccggg accgcagtcg
     1801 gcgtcgcagc cgtacacgtc gtcgtcgatg tactacaaca tgtacgtgcg ctgctgctcg
     1861 ccgcagtcgc ccaatccata tggcgacaac cttgacgaca cgttgatcca cgagacgtgg
     1921 tggaagaaca cggtgaccgc gcccgaccag ctgcgccagc gcgtggcgac ggcgtacagc
     1981 gagatcttcg tcgtctcgga aatcaacgac aacctcgacg ctcagatccc cggcatgtgc
     2041 acgtattacg acatgctggc cgacgatgcg tttgtcagct tcaagcagct gctgagcgac
     2101 atcacgctgc acccgatcat gggtgagtac ctgaacatgc gcgggaacaa gaaggcgacc
     2161 gcgccggcgg tgccgaacga aaactatggc cgcgaggtgc ttcagctgtt ctcgatcggc
     2221 ctgtacatgc tgcagccgga cggaacgttg atgctcgacg ccaacggcca accgatcccg
     2281 acgtacgacc agccgactat taccagcttc gcgcaggtct tcacgggttg ggatgtgaac
     2341 aataccggcg tgactgttcc cgaactgatc agcgacggcg cgggaggcgc gacgaccgcc
     2401 aacttcacca gcagatacca gaagccgatg gtgctcacgc agagcaacca ctcgaccacg
     2461 gtgaagaacc tgctggttta cccgggcgcc gcgacgtggt cgttcggcgg cgcgtcgggg
     2521 ccgtcgcaga tccccgccaa cagcggacag accgccagcg ttgagctgaa cttcgcgatc
     2581 gacaacatcg tcaaccaccc caacgtcggc ccgttcatct gccggcagct gatccaacgg
     2641 ctggtgggca gcaatcccag ccccgcgtat gtctatcgcg tggcgcaggt cttcaacaac
     2701 gacggcagcg gcaaccgtgg gaacatgaag gcggtcatca ccgccatcct gaccgattac
     2761 gaggcgcgca gcccggcggt cttgagcgat ccgggttatg gccacatgcg cgagccgatg
     2821 atccgcgtcg ccagcct
//
LOCUS       DATIMH010000983         1672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1682198, whole genome shotgun sequence.
ACCESSION   DATIMH010000983 DATIMH010000000
VERSION     DATIMH010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682198"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VLI90_17055"
     CDS             complement(<1..393)
                     /locus_tag="VLI90_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15973.1"
                     /translation="MPALYARKSPKRRTARQARNLDTSVAATPASPEVGTLMSRRDGT
                     IWYYLDDEASPQNGQLRSRTMEVRDGEPTVTVDAQPQSVRFEKRLQNHERDTGLRPVR
                     GALVVVERQIFEVQCIAHGPEARVTIQSP"
     gene            392..>1672
                     /locus_tag="VLI90_17060"
     CDS             392..>1672
                     /locus_tag="VLI90_17060"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSV15974.1"
                     /translation="MTELATESPLSNSIAFPPLDAGDLAALRPMAEPCSFDDGQIVFR
                     VGDADLDLYVVESGGLEIVNPSDDNRHIVTHGPGQFAGDIDLLTRRPVIVTAIARGPT
                     RLLRVPGARLRELLNKVPRFSEKLMIAAQERRRLLAESGVLGLKVVGPGKCRDTMLVR
                     EFLFKNFVPFTWYDSTSMRGQSLMNGWGSPKRSPVVECGDGHVLINPSLRDLAKGAGV
                     WRHCPGDSVDLAIIGAGPAGMTAAVYAASEGLSTIVLDRLGPGGQAGGSSKIENFIGF
                     PSGLSGADLATRSVLQMLKFGAKMIAPVTVEHIEPASSSASSEAPGFHALQLDCGTTL
                     RARTILLAAGVRWRKLEAEGAERFESAGIHYACTSVEAILYDTQDVAVVGAGNSAGQA
                     AMFLAECCRDRRVHLFVRRRLGPGMSDYLVGRIRA"
BASE COUNT          261 a          542 c          561 g          308 t
ORIGIN      
        1 cggcgattga atcgtgacac gggcctccgg cccgtgcgcg atgcactgga cttcaaaaat
       61 ctgccgttct actactacca aagcaccacg cacgggccgc aggcccgtgt cacgttcatg
      121 gttctgtaat cgcttctcaa atctgaccga ctggggttgc gcatccaccg tcaccgtcgg
      181 ctcgccgtct cgaacctcca tcgtccggga ccgcagctga ccgttctgcg gcgacgcctc
      241 atcgtccagg taataccaga tcgtcccatc ccggcgcgac atcagcgtgc cgacctcggg
      301 cgacgcaggc gtcgcggcta cagaggtatc cagattcctc gcctgacgcg ccgttcgccg
      361 cttggggctt tttctggcgt acaatgcggg catgaccgaa ctggcaactg aatcaccgtt
      421 gtccaactcc atcgcctttc ccccgctgga cgctggcgac ttggcggcgt tgcgaccgat
      481 ggcggagcca tgttcctttg atgacgggca gatcgtgttc cgcgtcggcg acgcggacct
      541 cgatctctac gtcgtcgaat ccggcgggct cgagatcgtg aacccatcgg acgacaaccg
      601 ccacatcgtc acgcacgggc cggggcaatt cgcgggcgat atcgacctgc tgacgcgccg
      661 gccggtgatc gtgacggcga tcgcgcgcgg gcccacgcgc ttgctgcgcg tgcccggggc
      721 gcgcctgcgc gagctgctga acaaggtgcc gcggttcagc gagaagctga tgattgccgc
      781 gcaggaacgc cggcggttgc tggcggagtc gggcgtgctc gggctgaaag tcgtcggtcc
      841 cggcaagtgc cgcgacacga tgctcgttcg cgaattcctc ttcaagaact tcgtgccctt
      901 cacgtggtac gactcgacat cgatgcgcgg gcagtcgttg atgaacgggt ggggatcgcc
      961 gaaaagatcg ccggtcgtcg aatgcggcga cgggcatgtg ctgatcaatc caagcctgcg
     1021 cgacctggcg aaaggcgccg gtgtttggcg gcactgtccg ggtgactcgg tcgatctggc
     1081 gatcatcggc gccggccccg ccggcatgac cgccgcggtt tacgcggctt ccgaaggtct
     1141 atcgaccatc gtgctcgatc gcctcggtcc cggcgggcaa gccggtggtt cgtcaaagat
     1201 cgagaacttc atcggcttcc ccagcggcct ctccggcgcg gacctggcaa cccgcagcgt
     1261 gctgcagatg ttgaaattcg gcgcgaagat gatcgcgccg gtgacggtcg agcacatcga
     1321 gcccgcgtca tcgtcggcgt cgtcggaagc gccgggattc cacgcgctgc aattggactg
     1381 cggcacaacg ctgcgggcgc ggacgatcct gctggccgcg ggcgtgcgct ggcggaagct
     1441 ggaagctgag ggggcggagc ggttcgagtc ggcgggcatc cactacgcgt gcacgtcggt
     1501 cgaggcgatc ctttacgaca cgcaggatgt ggcggtcgtc ggcgccggca attccgccgg
     1561 gcaggcggcg atgttcctgg ccgagtgctg ccgcgatcgc cgcgtgcatc tgttcgtccg
     1621 gcgccggctt ggtccgggca tgtcagacta tctcgtcggc cggattcgcg cg
//
LOCUS       DATIMH010000984         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3411242, whole genome shotgun sequence.
ACCESSION   DATIMH010000984 DATIMH010000000
VERSION     DATIMH010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3411242"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1401
                     /gene="lysA"
                     /locus_tag="VLI90_17065"
     CDS             118..1401
                     /gene="lysA"
                     /locus_tag="VLI90_17065"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSV15975.1"
                     /translation="MDFFNYRNNELYCEDVPAERIAREVGTPAYVYSKATLLHHYRQI
                     ADAFKPVNATICYSIKSCGNINILKVLAAEGCGFDVTSDGELYRALQVGADPKKIIYA
                     GVGKTDVEIANAIRAGIAAFNLESEAEIENIDRVAGSIGMQAVGAIRINPDVDPKTHV
                     KTTTGKKETKFGVDIERAERVFDQYRNLKNLRIGGVHTHIGSPVYEIQPYVDAVTKMT
                     DLIDRLTRRGHKIEWFDVGGGFGVNYERPDQAVPVTEHAKALLPLLSGKPYKIAFEPG
                     RYIAGNAGILLTRVLYRKTGGEKRFVIVDAGMNDLIRPTLYESYHHIWPVKPDAANIP
                     PERSRLFEPRDGETVDVVGPICESGDYLAKGRPLPPTKRGDLLAVFTAGAYGFAMSSN
                     YNNRPRAAEVLVDGASFKIIRRRETLEDLVALERV"
     gene            1568..2938
                     /locus_tag="VLI90_17070"
     CDS             1568..2938
                     /locus_tag="VLI90_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15976.1"
                     /translation="MKLNLCCRAAAIFFLTTALGLDAARGQADDAAALTSAPATQASV
                     PPARIDALIKQLNSDEWRDRETAQHELVAIGAPALDALRRTAESDEMDPEVRSRAAAA
                     MAAIHAQNLDGPTLLSMHYHDVGAPQVLDAIAEQAHVRFNTDVFPIAPTRTITVDADG
                     LPFWDVMEQVCTQLGVCPMPEGGGQSLRLQPMPQNWLTQAPHRVVGPFVVSVTEVHRF
                     RTVDLTGMRGTDERFGLQVMVNAEPKVMVAQMSGMEVTEAVDDAGHSLLPVARPGRGV
                     PGMFFRGNVRAGQQIDVPLQYPAADAGKKIARLRGDVKVAVGQNVERFQIDDPLGAMK
                     VSHALPGRGIEVQITKMSDDAFTVKVTLKRGNLPQEQWMAMTNRAFDIALEDADGHRM
                     TTNGWGGGGGGDEFNFTGQFTRNLMRVNGGRPQKTGQPVRLVWDVPTKVKNITVPVEF
                     KDLPMP"
     gene            complement(2983..>3206)
                     /locus_tag="VLI90_17075"
     CDS             complement(2983..>3206)
                     /locus_tag="VLI90_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15977.1"
                     /translation="LEQEILALRPRHRDESIHSLVAEMRQLLQRAVSTWATLGSECPP
                     TAQVFRDLTEALFKHGFLRRPPPRNLEEV"
BASE COUNT          617 a          916 c         1112 g          561 t
ORIGIN      
        1 gaaggatcta gctggttcgt gcaagccagc cagatgcttc gcggtacctc agcatgacag
       61 tgcttcggca tgtcaaacag tgacgcgcta ttcaggctga tttagaatca attccacatg
      121 gattttttca actaccgaaa caacgagctg tattgcgaag acgtgcccgc cgagcgaatc
      181 gcgcgggagg ttggcacgcc cgcatacgtc tattccaagg ccacgctgct gcatcactac
      241 cggcagatcg ctgacgcgtt caagccggtc aacgcgacga tctgctacag catcaagagc
      301 tgcgggaaca tcaacatcct gaaagtcctg gccgccgagg gatgcgggtt tgatgtgacc
      361 agcgatggcg agttgtatcg cgcgctgcag gtgggggcgg atccgaagaa gatcatttac
      421 gccggcgtgg ggaagacgga tgtcgagatc gccaatgcga ttcgcgcggg catcgcggcg
      481 ttcaatctgg aatcggaagc ggagattgaa aacatcgacc gcgtggccgg gtcgatcggc
      541 atgcaggcgg tgggggcgat tcgcattaat cccgatgtgg accccaagac gcacgtgaag
      601 acgacgacgg gcaagaagga aacgaagttc ggcgttgaca tcgagcgggc cgagcgcgtg
      661 ttcgaccaat atcgcaatct gaagaacctg cgtatcggcg gcgtgcacac gcacatcggt
      721 tcgccggtgt acgagattca gccgtatgtg gacgcggtga cgaagatgac ggacttgatc
      781 gatcggctca cccgacgcgg tcacaagatc gaatggttcg acgttggcgg cgggttcggc
      841 gtcaactacg agcggccgga ccaggcggtg ccggtgaccg agcacgccaa ggcgttgctg
      901 ccgctgttga gcggcaagcc atacaagatc gcgtttgagc caggtcgata catcgcgggg
      961 aacgcgggca ttttgctgac gcgggtgctg tatcgaaaga cgggtggcga gaagcgtttc
     1021 gtcatcgtcg atgcggggat gaacgatctg attcgcccga cgctgtacga gagctatcac
     1081 cacatctggc cggtgaaacc ggacgcggcg aacattccac cggagcgctc gcggctgttt
     1141 gagccgcgcg atggtgagac ggtggacgtg gtcggtccga tctgtgaaag cggcgattac
     1201 ctggcgaagg gccggccgct gccgccgacg aagcgcggcg atctgctcgc tgttttcacg
     1261 gccggggcgt acgggtttgc catgagcagc aactacaaca accgcccgcg tgccgcggag
     1321 gtgttggtgg acggagcctc gttcaagatc atccgccggc gcgaaacgct ggaggacctg
     1381 gtggcgctgg agcgcgtgtg atccactttc aaaatcgtgc gtagctgtgc gcgtagcggt
     1441 agccccgaag gggcatgcgt tttcgttcga aagaaacacg tgccccttcg gggctaccgc
     1501 tacgcatggc aatattggcg cgcccgctct tccccattgc cgcttgatct tcgttacaat
     1561 tgcgcccgtg aaactgaacc tctgttgtcg cgcggccgcg atctttttcc ttaccacagc
     1621 gcttggactc gacgctgcgc gaggtcaggc agacgatgcg gcagcgctga catcggcccc
     1681 ggcgactcaa gcgtccgttc cgccggcgcg gatcgacgcg ctgatcaagc agctcaacag
     1741 cgatgaatgg cgagatcgtg aaacggctca gcacgagctg gtcgcgatcg gcgcgccggc
     1801 gctggacgcg ctgcgcagga cggcggagtc ggacgagatg gaccccgagg tccgcagccg
     1861 ggcggcggcg gcgatggcgg cgatccacgc gcagaacctg gacgggccga cgctgctgtc
     1921 gatgcattat cacgacgtgg gcgcgccgca ggtgctggat gccatcgccg agcaggccca
     1981 cgtgcggttc aacacggacg tctttccgat cgcgccgacg cgcacgatca cggtggatgc
     2041 cgatgggctg ccgttctggg atgtgatgga gcaggtgtgc acgcagctgg gcgtgtgccc
     2101 gatgcccgag ggcggcgggc agtcgctgcg gttgcagccg atgccgcaga actggttgac
     2161 gcaggcgccg caccgggtgg tcgggccgtt cgtcgtttcc gtgacggagg ttcatcgctt
     2221 ccgcacggtg gatctcaccg gcatgcgcgg gaccgatgaa cggttcggcc tgcaggtgat
     2281 ggtgaacgcc gagccgaagg tgatggtcgc gcagatgtcc gggatggagg tgaccgaggc
     2341 ggtggatgac gcggggcatt cgctgctgcc ggtggctcga ccggggcgcg gcgtgcccgg
     2401 catgttcttc cgcggcaacg tgcgcgcggg gcagcagatc gacgtgccgc tgcagtaccc
     2461 cgccgccgac gcgggaaaga agatcgcccg ccttcgcggc gacgtgaagg tggcggtcgg
     2521 gcagaacgtc gaacgctttc agatcgatga tccactgggc gcgatgaagg tatcacacgc
     2581 gctgccgggg cgggggatcg aagtgcagat cacgaagatg tcggacgacg cgttcacggt
     2641 gaaggtgacg ctgaagcgcg gcaacctgcc gcaggaacag tggatggcga tgaccaaccg
     2701 cgcgttcgac atcgcgctgg aagatgccga cggccaccgc atgaccacca acggctgggg
     2761 cggcggtggc ggtggtgatg agtttaactt caccggtcag ttcacgcgca acctgatgcg
     2821 cgtgaacggc ggccgcccgc aaaagaccgg gcagcccgtg cggctggtgt gggacgtgcc
     2881 gacgaaggtg aagaacatca cggtgccggt ggagtttaag gacttgccga tgccgtgact
     2941 ccaccggcgc aaagcgagat gttcatcgag ccgctgtgtc ccttacacct cttcaagatt
     3001 ccgcggcggt ggacggcgca agaatccatg cttaaacagc gcctccgtca gatcccggaa
     3061 aacttgcgcc gtcggggggc attcggaccc aagcgttgcc caggtcgaaa ccgcacgctg
     3121 cagaagttgc cgcatttccg caacaagtga atggatggat tcgtccctgt ggcgcgggcg
     3181 tagtgccagg atttcctgct caagtg
//
LOCUS       DATIMH010000985         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2255964, whole genome shotgun sequence.
ACCESSION   DATIMH010000985 DATIMH010000000
VERSION     DATIMH010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255964"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1091)
                     /locus_tag="VLI90_17080"
     CDS             complement(204..1091)
                     /locus_tag="VLI90_17080"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HSV15978.1"
                     /translation="MTRTNLKVALLQETDHGGRDANLDAIEAGLREAARAGAELVLLQ
                     ELHNGTYFCQHESVEVFDQAETIPGPGTARIGKLAEELKLVVVASLFEKRATGLYHNT
                     AVVFDRSAAIAGVYRKMHIPDDPGFYEKFYFTPGDLGFEPIDTAVGRLGVLVCWDQWY
                     PEAARLMALAGAELLLYPTAIGWDPNDEQAEKDRQRDAWVTVQRGHAVANGLPLLACN
                     RTGHEADPCGGAGIQFWGSSFVAGPQGEFLARAGTDQRELLMVDIDLARSEHVRRIWP
                     FLRDRRIDAYGDLLKRYRD"
     gene            complement(1151..>1652)
                     /locus_tag="VLI90_17085"
     CDS             complement(1151..>1652)
                     /locus_tag="VLI90_17085"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HSV15979.1"
                     /translation="SREAMSGILRDNLHARRVLWLDYGYLEGDDTDAHIDTLARFAPG
                     ERIVFQACADPADPHYAELKRMADELAALRTDDGRAYQLCPLPWPQPIVDEGRRLAAS
                     YANYLIVNDAVLVPAYGDPADDAAAEVIGAAHLDRSIVQVPCRPLIWQNGSLHCITMQ
                     LPAGLA"
BASE COUNT          276 a          536 c          560 g          280 t
ORIGIN      
        1 cgccacgctg ctgcgcgaaa cgtgtgcggt gcccagcacc acgtagtcga cgccatcgcg
       61 cacgacccgc tcgatcggtt gatcctgcag ggcaggcggc agcggaatat cgagttgggg
      121 caagctcatg cgatcacgtg gtgttggaaa ccccaagcat ggggttggcc agcgcccgtc
      181 acaagcgcgg gcgagcaggg gactcagtcg cgatagcgct tgagcaggtc gccataggcg
      241 tcaatgcgcc ggtcgcgcag gaacggccag atccgccgca cgtgttcgct gcgtgccaga
      301 tcgatgtcga ccatcaacag ctcgcgttga tcggtgccgg cgcgggccag gaactcgccc
      361 tgcggcccgg ccacgaagct gctgccccag aactggatgc cggcgccgcc gcacggatcg
      421 gcttcgtgcc cggttcggtt gcaggccagc agcggcaggc cgttggcgac ggcatggccg
      481 cgctgcacgg tgacccaggc gtcgcgctgg cgatccttct cggcctgctc gtcgttcggg
      541 tcccagccaa tcgcggtggg atacagcagc agctcggcgc cggccagcgc catcaatcgc
      601 gccgcttccg gataccactg atcccagcac accagcacgc cgagtcggcc caccgcggtg
      661 tcgatcggtt cgaagccgag atccccgggc gtgaaataga acttttcgta gaaacccgga
      721 tcgtcgggaa tgtgcatctt gcgatacacg cccgcgattg ccgccgaacg atcgaacacc
      781 accgcggtgt tgtgataaag cccggtcgcg cgcttctcga acagcgaggc gaccaccacg
      841 agtttcagct cttcggcgag cttgccgatg cgcgcggtgc cggggccggg aatggtttcg
      901 gcttgatcga acacctcgac cgattcgtgc tggcagaaat acgtgccgtt gtgcagttcc
      961 tgcagcaata ccaattccgc gccggcgcgc gcggcctcgc gcaggccggc ctcgatcgcg
     1021 tccaggttgg cgtcgcggcc gccgtgatcg gtttcctgca gcagcgcgac cttgaggttc
     1081 gtgcgggtca tcagcgttct caacctggct tgttggtcag ccagcaaagt ctaacgggtg
     1141 gcgatgaagc tcaggccaat ccggccggca gctgcatggt gatgcagtgc aggctgccgt
     1201 tctgccagat cagcgggcgg cagggcacct gcacgatgct gcggtcgaga tgcgctgcgc
     1261 cgatcacttc agcggcggca tcgtcggcgg gatcgccgta ggcgggcacc agcaccgcat
     1321 cattgacgat caggtaattg gcgtacgaag cggccagtcg gcggccctcg tccacaatcg
     1381 gttgcggcca tggcagcggg cacagctggt acgcgcggcc gtcatcggtg cgcagcgcgg
     1441 ccagttcatc ggccatgcgc ttcaattcgg cgtagtgcgg atccgccgga tcagcgcagg
     1501 cctgaaacac aatgcgctcg ccgggtgcga agcgggcgag cgtgtcgatg tgcgcgtcgg
     1561 tgtcgtcacc ttcgaggtag ccgtagtcga gccacagcac gcggcgggcg tgcaggttgt
     1621 cacgcaggat cccgctcatc gcttcgcgcg at
//
LOCUS       DATIMH010000986         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3985161, whole genome shotgun sequence.
ACCESSION   DATIMH010000986 DATIMH010000000
VERSION     DATIMH010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3985161"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1343)
                     /gene="glyA"
                     /locus_tag="VLI90_17090"
     CDS             complement(87..1343)
                     /gene="glyA"
                     /locus_tag="VLI90_17090"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSV15980.1"
                     /translation="MNLEQADPQIFDLIQKEKRRQETTLELIASENHVSLAVLEAMGS
                     VLTNKYAEGYPRARYYQGCGYYDEVETLAIERAKQLFGCRFANVQPHSGAQANGSVFL
                     AMLNPGDTFASLVLADGGHLSHGRKVNMSGKWFNPVHYPLVYDASRADFEYLDYEAAR
                     KVCLEAKPKMILCGYSAYPRIIDFKKFRDIADECGAVLMADIAHIAGLIAGGAHPSPF
                     PHAHIVTTTTHKTLRGPRGGMIMTNDEELAQKIDKAVFPGMQGGPLMHVIAAKAVAFG
                     EDLKPEFRSYAQQIVKNAQSLAAALKAFGYRIVSGGTDNHLMLVDLRARNENFTGAEA
                     AVALEKAGIIVNFNGIPKDPRPAKLTSGIRLGTPALTTRGLKEADMRTVASFIDRALL
                     GKDDAAALAKVRDEVATFTQRFPMPH"
     gene            complement(1340..>1524)
                     /locus_tag="VLI90_17095"
     CDS             complement(1340..>1524)
                     /locus_tag="VLI90_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15981.1"
                     /translation="PRDQTPAVTDSYEMRKCEARRYFEGVKRGSPGKTLVQKRDVFLR
                     SLGIATILRFPWRDAE"
BASE COUNT          262 a          503 c          446 g          313 t
ORIGIN      
        1 tactcgcgac gacccggatt cttctcgcgc gtgaagcaaa acatccgggt cgtcggagta
       61 cctctgaacc cggcgtggtc ggctgatcaa tgcggcatcg gaaaccgctg cgtgaatgtc
      121 gcgacttcgt cgcgcacctt cgccagcgcc gccgcgtcgt ctttgccgag cagcgcacga
      181 tcgatgaacg atgccaccgt ccgcatgtcc gcttccttca atccgcgcgt cgtgagcgcc
      241 ggcgttccca ggcgaatccc gctggtcagc ttcgccggcc gcgggtcctt cgggatgccg
      301 ttgaagttca cgatgatacc ggccttctcc agcgccaccg cggcttcggc gccggtgaag
      361 ttttcgttgc gggcgcgaag gtcgaccagc atcaggtgat tgtctgtccc gccgctgacg
      421 atgcggtagc cgaaggcttt gagcgccgcc gccagcgatt gcgcgttctt cacgatctgc
      481 tgcgcgtagc tgcggaactc cggcttcaga tcttcgccaa acgcgaccgc cttggcggcg
      541 atcacgtgca tcaacggccc gccctgcatg ccggggaaga cggccttatc gattttctga
      601 gccagctcct cgtcgttcgt catgatcatc ccgccgcgcg gcccgcggag ggttttgtgc
      661 gtggtcgtcg tgacgatgtg cgcgtgcggg aacggcgacg gatgcgcccc gccggcgatc
      721 agcccggcga tgtgcgcgat gtccgccatc aacaccgcgc cgcattcatc ggcaatgtcg
      781 cggaattttt tgaaatcgat gatccgcgga tacgccgagt agccgcagag gatcatcttg
      841 ggcttggcct caagacaaac tttgcgcgcc gcctcgtagt cgaggtattc gaaatccgcc
      901 cggctcgcgt cgtacaccag cgggtaatga acggggttga accacttgcc gctcatgttg
      961 accttgcggc cgtgcgacag gtgcccacca tcagccagca cgaggctcgc gaacgtgtcc
     1021 cccggattca gcatcgccag aaataccgac ccgtttgcct gcgccccgct gtgcggctgc
     1081 acgttcgcaa agcgacagcc aaacaactgc ttcgcccgct cgatcgccag cgtctcgacc
     1141 tcgtcgtagt acccgcaccc ctggtaatag cgcgcgcggg gatacccctc ggcgtacttg
     1201 ttggtcagca ccgaccccat cgcctccagc accgccagcg aaacgtggtt ctcgctggcg
     1261 atcaactcca gcgtcgtctc ctgccggcgc ttttccttct gaatcaaatc gaaaatctgc
     1321 ggatccgctt gctcaaggtt cattcggcgt ctctccaggg aaagcggagg attgtagcga
     1381 tgccaagcga gcggagaaag acgtcccttt tttgcaccag ggtctttcca gggctaccgc
     1441 gtttgacccc ctcaaaataa cggcgcgctt cacatttgcg catttcatat gaatctgtta
     1501 cagcaggggt ttggtcgcga ggcc
//
LOCUS       DATIMH010000987         8349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4567014, whole genome shotgun sequence.
ACCESSION   DATIMH010000987 DATIMH010000000
VERSION     DATIMH010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4567014"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VLI90_17100"
     CDS             <1..391
                     /locus_tag="VLI90_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15982.1"
                     /translation="LCMIGGPAVVSLLRSKSETGSGVVQVKLTSYGFHQGTRQLGPIP
                     YQRCDEAALIPWSKAGFVQIADSPRRLHIKIAPTYIWWKFQARREFVNAEVSCTAVQQ
                     EALRARINKWRGIAGASASAPATEPDG"
     gene            complement(393..1301)
                     /gene="arsM"
                     /locus_tag="VLI90_17105"
     CDS             complement(393..1301)
                     /gene="arsM"
                     /locus_tag="VLI90_17105"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HSV15983.1"
                     /translation="MSNITEAVRSKYGATATSGLTNDHDGVRAVAEAFGYSAAELASV
                     PAGANMGLSCGNPTATASLKPGEVVLDLGCGGGLDVFLAAQKVGPTGKAIGIDITPEM
                     IERARRNAATAAGGEPLNNVEFLLGTIDHLPLPDASIECIISNCVINLAPDKPAVFRE
                     MFRVLKPGGRVAVSDIALKKALPPEIGADLMAYVGCIVGAIRIDEYRHWLTDAGFAHV
                     AVIDSGTDLSAYSKVEGQAGCCSPATTATTAAMSKSSSLPIATGCCGGRASHDQGTVH
                     GGLKELLMRYDVNEYAASVKIYAMKT"
     gene            complement(1298..1477)
                     /locus_tag="VLI90_17110"
     CDS             complement(1298..1477)
                     /locus_tag="VLI90_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15984.1"
                     /translation="MSKLKLLFLCTGNSCRSQMAAVSGQLTSLWVYLAGPVLGSAIAV
                     PCWRITRPSTNEEKP"
     gene            complement(1548..1910)
                     /locus_tag="VLI90_17115"
     CDS             complement(1548..1910)
                     /locus_tag="VLI90_17115"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSV15985.1"
                     /translation="MKTTAPINHVDLTFRAFSDRTRLRILNLLRGGELCVCDLIAVLA
                     VPQAKVSRHLAYLRRAGLVRVRKDGLWSHYSLAPASGAFHTKLLECLACCFADVPELA
                     KDAAKLNRQSKCGPKCCE"
     gene            1983..3611
                     /locus_tag="VLI90_17120"
     CDS             1983..3611
                     /locus_tag="VLI90_17120"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV15986.1"
                     /translation="MSLGGAATPATADFSAAANQTIASLTAPTNSTSANTLALGSKTL
                     NISSFKTGLVNPSVTIRGSAGGTSRVTNFIIADGNIYTNGGSTAQSGTVDLTAGTVDA
                     LINNLNVGVGKASSNTAGSTGLLTYNTGTIDANTVADGIRGTTTAAAASTGTITLGGT
                     AVLNVNAGGLKLADTSVGASTGTTTGILNINGTSTVNMVGGDILGGGGTSTITLNGGT
                     LDLQYHNLDTSGGPIANLNLQTGTLKNVLEINGGGVGLTKTVAGTLIVDGVNAYTGNT
                     NVNAGTLALAAGGSITGSTTITVASGAIANINGTVPTNATVHANGTTNFGGTTGNALL
                     TRTLAALNIAGGVTASITPSTNAFTPTFLSPTSLSFGDGSAKLDLTNNAIIATGDQDV
                     ALTLIQSGQVFSSTLADPNHALGYTSVPSSSNMEIRYTFKGDTKLDGKVNVGDLGALA
                     TNYNTTGGMSWANGDFNQDHTVNVADLGALATNYNVQLGSGSSSAGGLSAEPLAVVAA
                     SALSSAAVPEPSTFALLGLGALGQMSRRPRRHNV"
     gene            complement(3724..3933)
                     /locus_tag="VLI90_17125"
     CDS             complement(3724..3933)
                     /locus_tag="VLI90_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15987.1"
                     /translation="MHEKANSDREIIVLKAADGSRYVMVVRANGSLAIARDGELLSDK
                     HWNAADVDDCVKALYKSAGRAQPTS"
     gene            4229..5014
                     /gene="scpB"
                     /locus_tag="VLI90_17130"
     CDS             4229..5014
                     /gene="scpB"
                     /locus_tag="VLI90_17130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HSV15988.1"
                     /translation="MMRLVRDYDEDVDSITREAMASEESAGEEMVGSRPKRGKRRGAV
                     DAGAAPGTSADAEMRDGQVEDAPKAEVEAEGAEGEESSSAAIETVVDLPVLEALLLST
                     HHPLTAGRLAELMDLPGTKPIRQAIKQLNEQYEQSGRSFRIEQVAGGYQILTLPQFGD
                     YLKKLHQKEIDAKLTKAALETLAIVAYKQPILRADVEAIRGVACGETIRSLMEKHLVK
                     IAGRAEEPGRPILYGTTKRFLELFGLNSLKDLPKSETLPPQQA"
     gene            complement(5079..6068)
                     /locus_tag="VLI90_17135"
     CDS             complement(5079..6068)
                     /locus_tag="VLI90_17135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV15989.1"
                     /translation="MSVAVAEPQNAARTRCMLCGQDDAWHTHLSRVLDYLTGEYFDID
                     RCESCGLMITRPMPTEAQIERYYPPRYRNNRHAFTGSMRVALRARAIESFFPNGFRGR
                     LLDVGCGDGSFVMEMRRRGWDVCATELDPATVHRLQEQGVDARQSQGAFEHPFREPFD
                     AVTCWHVMEHVDRPLQVAEWVHTQLKPAGLFQATVPNAQSLQARLFKKQWLHLDVPRH
                     RYHFCPSTFESLLRRARLEPVGRTMFAWEYDWFGAIQSALNEVCGTPNWLFERLTRAD
                     VGPSTTTERSHPLDGAVSCLLAGPIALASLPAMLIARLFGDGATLTITARPAK"
     gene            complement(6065..6922)
                     /locus_tag="VLI90_17140"
     CDS             complement(6065..6922)
                     /locus_tag="VLI90_17140"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV15990.1"
                     /translation="MIHGKRLVVVMPAYNAEQTVERTFRDIPEGFVDDIVLVDDASRD
                     KTAEAAQRLGFHVLVHPRNRGYGGNQKTCYREALWLKGDVIVMLHPDYQYSPKLVVAM
                     ASMIATGQYDVVLASRILASSPMQGGMPLYKYVMNRILTLIQNILMRAKISEYHTGYR
                     AFSRQVLETLPLEENDDDFVFDNEMLAQAHYFGFRIGELSCPARYEPQSSSISLKRSI
                     KYGFGVLWTTVRYRLQKWRLVNDPIFNRDGRRLPPSPTEPARETAPPVVPPPVTASGS
                     EPVAEAARS"
     gene            7257..>8349
                     /locus_tag="VLI90_17145"
     CDS             7257..>8349
                     /locus_tag="VLI90_17145"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSV15991.1"
                     /translation="MDTVRIFDTTLRDGEQSPGATLTLPEKVEIARHLEAMGVDIIEA
                     GFPIASVGDFESVKTIAGEITKSTICGLARSTPKDVERAGEAVKGAAKSRVHVFCATS
                     KIHREHKLKKGKEDIIRISVESIKQALQYTKDVEFSPEDASRTEMEFLEEITQAAIEA
                     GATTINLPDTVGYATPKSYSKIFSHLIKKLPIIRERNIILSSHCHNDLGLAVANSLAA
                     VEGGARQVECTINGIGERAGNAALEEIVMALRTRGDFFKIGTRIDSTKIYTASRMVST
                     LTGLMVQRNKAIVGENAFAHEAGIHQDGVLKHRETYEIMDPATVGIPKNSLVLGKHSG
                     RHAFRERVIELGYTLSDEQVESAFVKFKAL"
BASE COUNT         1632 a         2564 c         2698 g         1455 t
ORIGIN      
        1 gctctgcatg atcggcggcc ctgcggtggt ttcgcttttg cgaagcaaat ccgagacggg
       61 ttccggtgtg gttcaggtga agttgacgag ctacgggttt caccagggca cgcgacaact
      121 cgggcccatc ccgtaccagc gctgcgatga ggcagcgctg atcccgtgga gcaaagcggg
      181 gtttgtgcaa atcgcggaca gcccgaggcg gctgcacatt aagattgcgc cgacatatat
      241 ctggtggaaa ttccaggcgc ggcgtgagtt cgtgaatgcc gaggtttcct gcacagcagt
      301 gcagcaggaa gcgctgcggg cgcgaattaa caaatggcgc ggcattgccg gtgcatctgc
      361 ctccgcgccg gcgactgaac ccgatgggtg atctacgtct tcatcgcgta gattttcacg
      421 ctcgccgcgt attcgttcac gtcgtagcgc atcagcagtt ccttcaagcc gccgtggacg
      481 gtgccttgat cgtgcgatgc tcgtccgccg cagcagccgg tggcgatggg gaggctgctc
      541 gattttgaca tcgccgccgt cgtcgccgtc gtcgctggcg aacagcagcc ggcttggcct
      601 tcgactttgg aatatgcgct tagatccgtg ccgctgtcga tcacggcgac gtgggcgaag
      661 ccggcatcgg tgagccagtg gcgatactcg tcgatgcgga tcgcgccgac gatgcaaccg
      721 acgtacgcca tcaagtccgc gccgatctcc ggcggtagcg ctttcttcag tgcgatatcg
      781 ctgaccgcga cccggccgcc gggcttgagc acgcggaaca tctcgcggaa aacggccggt
      841 ttgtccggtg ccagattgat cacgcaattg ctgatgatgc actcaatcga ggcgtcgggg
      901 agcggcaggt gatcaatcgt gccgaggagg aattcgacgt tgttcagcgg ctcaccaccg
      961 gcggcggtgg cggcgtttcg ccgggcgcgt tcgatcatct ccggtgttat gtcgatgccg
     1021 atcgccttgc cggtcggtcc aactttctgt gcggcgagga acacatcgag ccccccgccg
     1081 cagcccaggt ccagaacgac ctcgcccggc ttgaggctgg ccgtcgcggt gggattgccg
     1141 caggacaggc ccatgttcgc gccggcggga acgctggcca gctcggcggc gctgtaacca
     1201 aacgcctcgg ccacggcgcg aacgccgtcg tgatcgttcg tcaaaccgct cgtggcagtt
     1261 gcgccgtact tcgatcgaac cgcttcggtg atattgctca tggtttctcc tcgttggtag
     1321 atggacgggt gattcgccag catggaacgg cgattgccga gccgagcacc ggcccggcca
     1381 ggtagaccca cagcgacgtg agttgacccg acacggcggc catctggctg cggcatgaat
     1441 tgccggtgca gaggaagagc agtttcaact tcgacattgc gttacctttc actcgccaca
     1501 ctacattcgc tcacgcgaat gtgccggagc aaaaaagaga gacgccgtta ctcgcagcac
     1561 ttcggcccgc acttggactg gcggtttagc ttcgccgcgt ccttcgccag ctccggcaca
     1621 tcggcgaaac agcacgccag gcattccagc agcttggtgt gaaacgcgcc gctggcgggg
     1681 gccagggaat aatgactcca caatccgtcc ttgcgaactc gcacaagacc cgcgcggcgg
     1741 agatacgcca ggtgtcgcga aactttcgcc tgcgggacgg ccagcacagc gatcagatcg
     1801 cacacgcaca attccccgcc gcggagcagg ttgaggatgc gcaatcgcgt gcggtcggag
     1861 aacgcgcgga acgtgaggtc aacgtggttg atcggcgcgg tcgtcttcat attcgcttat
     1921 gcgattgtag tggcggcggc gcgttggggc aagtaccgga gacttgtagc tttgcttttt
     1981 gaatgtcgct cggcggcgcg gcgacgccgg ccaccgccga tttttcggcg gcggcgaatc
     2041 agacgatcgc cagtttgacg gccccgacca acagcacctc ggccaacacg ttggcgctgg
     2101 gcagcaagac gctcaacatc agcagcttta agacgggctt ggtcaatcca agcgtgacga
     2161 tccgcgggtc ggccggtggc acgtcgcgcg tgaccaactt catcatcgcc gacggcaaca
     2221 tctacacgaa cggcggcagc accgcccaga gcggcacggt tgatctgacc gccggcacgg
     2281 tcgatgcgct gatcaacaac ttgaacgtcg gcgtcggcaa ggccagttcc aacaccgcgg
     2341 gcagcactgg gctgctgacg tacaacaccg gcaccattga cgctaacacc gtcgcggacg
     2401 gcatccgcgg caccacgacc gccgcggccg cgtccaccgg cacgatcacg ctcggcggca
     2461 ctgccgtgct gaacgtgaac gccggcgggt tgaagctcgc ggacacgtcc gtcggcgcca
     2521 gcaccggcac tacgaccggt atcttgaaca tcaacggcac gtccaccgtc aacatggtcg
     2581 gcggcgatat cctcggcggt ggcgggacca gcacgatcac gctcaacggc ggaacacttg
     2641 acctgcagta tcacaacctg gacacgtcag gtgggccgat tgccaatctg aatcttcaaa
     2701 cgggcacgct gaagaacgtg ctggagatta acggcggcgg cgtcgggttg accaagacgg
     2761 tggccgggac gttgatcgtc gatggcgtca acgcgtacac cggcaacacc aatgtgaacg
     2821 ccggcacgct ggcgctggcc gccggcgggt cgattaccgg cagcacgacg atcaccgttg
     2881 ccagcggcgc catagcgaac atcaacggca ccgtgccgac gaacgcgacg gtgcacgcca
     2941 acggcacgac caatttcggc ggcacgaccg gcaacgcgct gctcacacgg acgctggcgg
     3001 cactcaacat cgccggcggt gtgacggcgt cgatcacgcc gtcaaccaac gcgttcacgc
     3061 cgacgttcct gtcgccaacg tcgctcagct tcggtgacgg cagcgcgaag ctggacctga
     3121 cgaacaacgc catcatcgcc acgggcgatc aggatgtggc gctgacgctg atccaaagcg
     3181 ggcaggtgtt cagctccacg ctggccgacc ctaatcacgc gctgggttac accagcgtgc
     3241 cgtcgtcgag caatatggag atccgctaca cgtttaaggg cgataccaaa ctggacggca
     3301 aggtgaacgt cggcgacctg ggtgcgctgg cgacgaacta caacaccacc ggtgggatga
     3361 gctgggcgaa cggtgacttc aaccaggacc acaccgtcaa cgttgccgac ttgggcgcgc
     3421 tggcgacaaa ctataacgtg cagctgggaa gcggatcgtc atcggctggc ggcctgagcg
     3481 ctgagccgct ggcagtcgtt gccgcgagcg cgttgtcatc ggcggcggtg cctgagccgt
     3541 ccaccttcgc gctgctggga ctgggcgcgc tgggtcagat gtcacggcga ccccgccggc
     3601 acaacgtgtg aggaacagtt tttggaatcc ctccgctgcg gcggctcgtc tctgaccagc
     3661 gggccgccac tactttttct gggcttcagc acacgctaac cgctgaagcg gttagcccgg
     3721 gcatcaactg gtgggttggg cgcgaccggc gcttttgtaa agcgctttga cgcagtcatc
     3781 gacatcagcg gcgttccagt gtttgtcgct cagcagttcg ccgtcgcgcg cgatggcgag
     3841 cgagccgttg gcgcgcacga ccatcacata gcgcgacccg tcagcggctt tgaggacgat
     3901 gatctctcga tcgctattcg ctttttcgtg cacgcgcgct gcccgtaata ttccaaagcc
     3961 cacagcccac cgccgcgcgg atgtgtgcgc gcgggcgatg caagcgcgta gagtgccatt
     4021 gccacggtgg cggggtcaat gctcgtcgaa aatccttagc gctctccgag actgattgtg
     4081 agttggctaa cagcgtcgcc gcgggcttgt gccagcatgg cggcgccggt atcaagtgtt
     4141 ggcagacatc atgggtcggt aaaacgctgc acaagccgcg ggccacgggc cgataatgtg
     4201 cgtagcgcgc cgaaaacgga gatggcccat gatgcgactg gttcgggatt acgacgagga
     4261 cgtagacagc atcacccggg aagccatggc aagcgaagag tcggcgggcg aggagatggt
     4321 cggatcgagg cccaaacgcg gcaagcggcg cggcgcggtt gacgccggcg ctgcgccggg
     4381 aacatcggcg gacgcggaga tgcgcgacgg gcaagtagaa gacgcgccca aggcggaggt
     4441 cgaggcggaa ggcgccgagg gcgaggagtc gtcatccgcg gcgatcgaaa cagtcgttga
     4501 tctgccggtc ctggaagcgc tgctgctgag cacgcaccac ccgctgaccg cggggcgatt
     4561 ggcagagttg atggacctgc cggggaccaa gcccattcgc caggcgatca agcagctgaa
     4621 cgagcagtac gagcagagcg ggcgaagctt tcgcatcgag caggtggcgg gcggatatca
     4681 gatcctgacg ctgccgcagt tcggcgatta cctgaagaag ctgcatcaga aggagatcga
     4741 cgctaagctg acgaaggcgg cgctggagac gctcgcgatc gtcgcgtaca agcagccgat
     4801 cctgcgggcc gacgtcgaag cgatccgcgg cgtcgcctgc ggtgagacga tccgcagcct
     4861 gatggaaaag cacctggtga aaatcgccgg ccgagcggaa gaaccaggcc gaccaatttt
     4921 gtacgggacg accaagcggt tcctagagtt gttcgggctg aacagcctga aggacctgcc
     4981 caagtccgaa acgctcccgc cgcagcaggc gtgaggaggc gatcggaagt ttaagcagac
     5041 cgcgaagagg agccccgcat acacatgcgg ggcttcgttc atttcgccgg acgcgccgtg
     5101 atcgtgagcg tggcgccgtc gccaaacaga cgcgcaatca gcatcgccgg caggctcgcc
     5161 agcgcgattg gtccggccaa caggcatgac acggccccgt cgagcgggtg cgatctttcc
     5221 gtggtggtgg acggtcccac gtccgcgcgc gtcagccgtt cgaacagcca gttgggcgtg
     5281 ccgcacactt cgttgagcgc gctttgtata gcgccaaacc agtcgtattc ccacgcgaac
     5341 atcgtccgtc ccaccggctc aagcctggcc cgacgaagga gggattcgaa cgtgctcggg
     5401 cagaaatggt agcggtgccg cgggacgtcc aggtgcagcc attgcttctt gaacaatcgc
     5461 gcttgaagcg actgcgcgtt cggcacggtc gcctggaaca gccccgccgg tttgagctgc
     5521 gtgtgcaccc actcggccac ctgcagcggg cgatcgacgt gctccatcac gtgccagcat
     5581 gtgaccgcgt cgaagggttc cctgaaaggg tgctcgaatg cgccctgcga ctgccgggcg
     5641 tccaccccct gctcctgcag gcgatgaacg gtggccgggt ccaactccgt cgcgcatacg
     5701 tcccaaccgc ggcggcgcat ctccatcacg aatgacccgt cgccgcaacc gacgtcgagc
     5761 aaccgtcctc gaaagccgtt ggggaaaaag gactcgatgg cgcgggcgcg taacgcgacc
     5821 cgcatcgacc cggtgaacgc gtggcggttg ttgcgatagc gcggcgggta ataacgctcg
     5881 atctgcgctt cggtcggcat cggccgggtg atcatcaacc cgcagctttc acagcggtcg
     5941 atgtcaaaat attcaccggt gaggtagtcg agcacgcgcg acaggtgcgt gtgccacgca
     6001 tcgtcctgtc cgcacagcat gcagcgcgtg cgcgcggcgt tctgaggttc ggcgaccgca
     6061 acgctcacga gcgcgccgcc tccgccacag gttctgaacc cgaagccgtc acaggcggcg
     6121 gcaccaccgg cggcgcagtt tctcgcgctg gctcagttgg actcggcggt aagcgccgcc
     6181 cgtcgcggtt gaagatcggg tcgttcacca gccgccattt ctgcaggcga taacggacgg
     6241 tggtccacaa cacgccgaag ccatacttga tgctgcgctt gagcgaaatg gacgacgact
     6301 gcggctcata gcgcgccggg cacgacagct cgccaatgcg aaagccgaag taatgcgcct
     6361 gcgccagcat ctcgttgtcg aagacgaagt cgtcgtcgtt ctcctccaac ggcagcgtct
     6421 cgaggacttg gcggctgaag gcgcgatagc cggtgtgata ttcggaaatc ttcgcccgca
     6481 tcaggatgtt ttggatgagc gtgaggatgc ggttcatcac gtatttgtac agcggcatcc
     6541 cgccctgcat gggtgagctg gcgaggatgc gcgacgcgag cacgacgtcg tactgccccg
     6601 tggcgatcat cgacgccatc gcgacgacca gcttcggcga atattgatag tcggggtgca
     6661 gcatgacgat cacgtcgccc ttgagccaaa gggcttcgcg atagcacgtc ttctggttgc
     6721 cgccgtaacc gcggttcctg ggatgaacca gcacgtggaa gcccagccgc tgggcggcct
     6781 cggcggtctt gtcgcggctg gcatcatcga ccagcacgat gtcatcgacg aacccctcgg
     6841 ggatgtcgcg gaaggtgcgc tccaccgtct gctcggcgtt gtacgccggc atcacaacga
     6901 ccaatcgctt gccgtgaatc actgaagaaa cactccgtgc tgttggtggc ggcgacgcct
     6961 gcgtcgcctt cgccacagga ggcgacgcag gcgtcgcggc tacaggaatc attcatctta
     7021 ccacgagata atccgccacg cgctattgac tttgcccgcg ctagccgatc tattatgcag
     7081 acaaatgcgg cagacttccc tgcaatcact gcgacttgcc ctcggccttc tgggccttga
     7141 gctcgttcgc gcatgatcgt agggaagctg ctcagaagaa ttttctatcg gccctacgat
     7201 catgtgatcg tggggtttct tgttttatag gcctcctttt cacaggagca gtggcaatgg
     7261 acacggtgcg aatcttcgac acgacgttac gcgatggcga gcaatctccg ggcgccacgc
     7321 tgacgcttcc ggaaaaggtc gagatcgccc gacacctcga ggcgatgggc gtggacatca
     7381 tcgaagcggg gtttcccatc gcgtccgtgg gcgacttcga atcggtgaag acgatcgccg
     7441 gcgaaatcac caagagcacg atctgcggcc tggcgcgatc gacgccgaag gatgtggagc
     7501 gcgcgggtga agcggtcaaa ggtgccgcca agtcgcgcgt gcatgtcttc tgcgcgacca
     7561 gcaaaattca tcgcgagcat aagctgaaaa aggggaagga agacatcatc cgcatcagcg
     7621 tcgagtcgat caaacaggcg ctgcaataca cgaaagatgt ggagttttcg ccggaggatg
     7681 cgagccggac ggagatggag tttctggagg agatcacgca ggcggcgatc gaggccgggg
     7741 cgacgacgat caacctgccc gacaccgtcg gctatgcgac gccgaagtct tacagcaaaa
     7801 tcttctcgca cctgatcaag aagctgccga tcatccgcga gcggaacatc atcctctcgt
     7861 cgcattgtca caacgatctt ggcttagccg ttgccaactc gttggcggcc gtcgaaggcg
     7921 gcgcgcggca ggtcgagtgc accatcaacg gcatcggtga gcgcgccggc aacgcggcgc
     7981 tggaagagat cgtgatggcc ctgcgcacgc gcggggactt cttcaagatc ggcacgcgca
     8041 tcgactcaac gaagatctac accgccagcc gaatggtcag cacgctgacc gggctgatgg
     8101 ttcagcgcaa taaggcgatc gtcggtgaaa acgccttcgc ccacgaagcc ggcattcacc
     8161 aggacggcgt gctgaagcat cgtgaaacgt acgagatcat ggaccccgcc accgtgggca
     8221 ttcctaaaaa ttcgctggtg ttgggcaaac attccggccg gcacgcgttc cgcgaacgcg
     8281 tgatcgagct gggttacacg ctaagcgacg agcaggtcga gtcggcgttc gtcaagttca
     8341 aagcgctgg
//
LOCUS       DATIMH010000988         7289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1395423, whole genome shotgun sequence.
ACCESSION   DATIMH010000988 DATIMH010000000
VERSION     DATIMH010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395423"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="VLI90_17150"
     CDS             complement(<1..1402)
                     /locus_tag="VLI90_17150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HSV15992.1"
                     /translation="MAARDGARVAYVDAHCGATLSYADLWRHARTLAASLTRDLPAGA
                     TVLLQSGNTLAFPIWFLAIVSGGLNCLPVHPDLTETELRDLAARTKAVAIAGCEGMIE
                     RLGDSIRLAMPLSRCWGAQQLNKHATVEKAGPTPGSERTRDDPDSSFFARQNPARRST
                     SEPGVAGADPFQRLHGHSDLLSVAPGSLLLSSSGTTGLPKVVWRSAVSLDAVSDAIVA
                     AIGFRADDIVLASVPLSHSYGIEHGLLAPLWAGSTVHLCDGFNIAIIARALAERATIF
                     PAVPAVIDMLATLNDVPATMPKLRLAYSAGGMLPAAVHERFAQRFGTHIGQVYGMTEI
                     GSVTFSDPRSADFSSDSVGRPMHGVSIRILDADPGADGEVAVRSPFMLDRYVDEEALL
                     IDGHFRTGDIGRIDARGELRITGRVRLLIEVGGRKVNPLEVEAVLSSHPDVNECVVVA
                     MKQTDTIHRLRAVLVAR"
     gene            complement(1437..2759)
                     /locus_tag="VLI90_17155"
     CDS             complement(1437..2759)
                     /locus_tag="VLI90_17155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSV15993.1"
                     /translation="MPSTTTKKDYDVIIIGGGPAGSTAAIVLARAGFSVGLFERATHP
                     RFHIGESILPRNMAMIRELGLEEQLRKLPHVPKYGAEFAMGDDLKTMQFRFADGLLPG
                     DVVFNIERSGFDKMLIDNARSAGAAVFEKTAVRAIDRLSDGDVAVTTDNGPVTARVLL
                     DASGHGTVVGRHLKQRRNFDDPELQKVAYFQQFDDVERPPGDASGDPTIVMADEGWFW
                     LIGLTDRRTSVGFVTRPWFVRDLRVPPDRLLQWAIARCPVVRHRMRAAVGPATNDVLS
                     DFSYTCSPIAGPGYFMIGDAGCFLDPIFSTGVTLAMVGGNEAAKLTIAMLRGSKTAAA
                     AQREYVRFVEGSTAVFWRLIRGYYRHSFRELFMHGRGPHNVHGAIISTLAGSVFPRPP
                     WALRWRMRLFELCVWLQQYIALVPRRRRFRLLDEQPVEPQLHAVPSHA"
     gene            complement(2769..3539)
                     /locus_tag="VLI90_17160"
     CDS             complement(2769..3539)
                     /locus_tag="VLI90_17160"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15994.1"
                     /translation="MTTRVDGFRHGDPQRLSQQVIDAYEQVRQALCESPHQHIVRMWN
                     HIPGIHDPLNPGQDRYMLFNAGRFAAMRMWFGAAEEIRRCVPAASGVGHDGDDLVIHA
                     LAMATPGVAVENPDQVPAYHYSPRYGPVPPCFARATRVMSPMSTLFISGTAAIIGEES
                     RFGADLEKQFELTLRNLSIVIAKARDDDHRIESPPRPSPLETLQHIRIYVPPGVDQQA
                     VDRLARSAFVESTSIEWVSAELCRSDLLVEIEGVARLS"
     gene            complement(3817..6189)
                     /locus_tag="VLI90_17165"
     CDS             complement(3817..6189)
                     /locus_tag="VLI90_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HSV15995.1"
                     /translation="MQLRSDANSAAAIAITGVGLTTALGATAEDTWQAVLRGDAQPAP
                     MPDIESPLPVGAAGHQAVDLPRDYQPQLPRETRYLRWTIESALRDAGLSSPSARQAIP
                     PARRAVAIGTTLHGMRAGGRFYRSEDAGELGTFLAGDVLRLALDDLDFAGAATTSCSA
                     CSSGLGSVALAATLLQTGAADLVVAGGYDTVSEYVWAGFNSLRLVADGPLRPFSRDRR
                     GMKLGEGYAILVLERLEDAKRRGANVCGIIAGWAESADAHHLTQSHPKGDGAARAMRV
                     AMLRAGVAPSEVGLVAAHATGTPDNDAAEHAALSQVLGERLPQTPVVCFKTHLGHTLG
                     AAGAAELIMSTMALRDGVIPPTANFDPAQLEFERLNVTHGAARKVPLQRTMNMSLGFG
                     GANGCVVLAKPPAAVEVADTNTPASKLALREVWITGIGVVLPGAVGIDAFAAKLKDDA
                     AAPAWVSSPPPLADSDFEHLLNARRVRRMSRYTTIMLAAAQQACGDARLLDDPALLAE
                     TGAMLGTTHGAVNFCHEYYQQIVRDGVLAANPVLFAEGVPNVGAAQLSLMLGLKQSCQ
                     SIIGTRTAGLDALRLAWLRIAAGQAERIIVGAGEEDSPLIERVYEHCGLRRTRDLGPA
                     YASTAGFRTASGAVAFVIESADAARARGVEPYAKLGHAAAARGTPDALPRTLASVLRD
                     APREAQWVGSGCGTWIDKLEMNALTRDDGVRAVGSIYSRFGEAFSTTPLISAAAAIVL
                     GHLPDATPTPAAARAVGRTDRFGVICTDWQGGVATAHLELGQRKPQTKPA"
     gene            complement(6164..7132)
                     /locus_tag="VLI90_17170"
     CDS             complement(6164..7132)
                     /locus_tag="VLI90_17170"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSV15996.1"
                     /translation="MNARGDADDVKPPPQPAWVRACLALFFFLGEHAGWVMRWIRPLV
                     PPITLWSSRSVAENLRLNAACIFGRPLPAAEQRDFARGVIRNFYDFVVELAASGRAST
                     AEILSRIEGVDGEPAYLEARKHKRGVILVTAHVGAFEVGLAALRRVESRVHVVFKRDD
                     FAGFERIRTQVRKQLEVREAAIDGGLPALMALRDALLANEAVVMQGDRAMPGERAAVV
                     PFLHGRLRMPIGPVKLARLTGSPLVPVIVLRGGRGRFRIHLGEPVYVQEGENSASDET
                     DPALLSLARTFESFVRQHPQQWLVLDRAFVDESSEEKRRAVEERCK"
BASE COUNT         1285 a         2448 c         2380 g         1176 t
ORIGIN      
        1 cgcgcgccac cagaaccgcg cgcagtcgat ggatcgtgtc cgtctgtttc atcgcgacga
       61 cgacgcattc gttcacgtcg gggtgactcg acagcaccgc ctccacttcc agcgggttga
      121 cctttcgccc gccgacttcg atcagcaggc gcacccgacc ggtaatgcgc agctcgccgc
      181 gcgcgtcgat ccgcccgatg tcgccggtgc ggaaatgacc gtcgatcagc agcgcttctt
      241 catcgacgta tcggtcgagc atgaacggcg atcgaacggc gacctcgcca tcggcgccgg
      301 gatcggcgtc gaggatccga atcgacacgc cgtgcatcgg cctgccgacg ctgtcggacg
      361 agaagtccgc tgagcgcgga tcgctgaacg tgaccgagcc gatctccgtc atcccgtaca
      421 cctgcccgat gtgcgtgccg aagcgctggg cgaagcgctc atgcaccgcc gccggcaaca
      481 tgccgccggc ggaatacgcg aggcgaagtt ttggcatcgt cgccggcaca tcgttcaacg
      541 tcgccagcat gtcgatcacc gcgggcaccg ccgggaagat cgtcgcgcgc tcggcgagcg
      601 cgcgggcgat gatggcgatg ttgaatccgt cgcacaggtg cacggtgctg cccgcccaca
      661 gcggcgcgag cagaccatgt tcgatcccat acgaatgcga caacggcacc gacgccagga
      721 cgatatcgtc cgcgcgaaat ccaatggccg cgactatggc gtctgacacg gcgtcgagag
      781 aaaccgccga ccgccacaca accttgggca atcccgtggt gccggaactg ctcagcaaca
      841 gcgatccagg cgcgactgac agtaaatccg aatggccatg gagacgttga aaagggtcgg
      901 ctcccgccac gccgggttcg gaggtactcc gacgggccgg attctgccgc gcgaaaaaag
      961 aagaatccgg atcgtcgcga gtacgctccg aacccggcgt gggaccggct ttttcgacgg
     1021 tcgcgtgctt atttagctgc tgtgcgcccc aacagcgcga caacggcatg gcgaggcgga
     1081 tcgaatcgcc cagccgctcg atcatccctt cgcatccggc gatcgctacc gctttcgtcc
     1141 gcgcggccaa atcacgcagt tccgtttcgg tcaggtccgg atggaccggc aggcaattca
     1201 gcccgccgct gacgatcgcg aggaaccaga tcggaaacgc cagcgtgttg ccgctctgca
     1261 gcagcacggt tgccccagcc ggcagatcgc gcgtgagcga tgctgccagc gttcgggcat
     1321 gtcgccacag atccgcgtag ctcaacgtcg ccccgcagtg cgcatcgacg tacgccaccc
     1381 gcgcgccgtc gcgagcggcc atgtcgcgca ctcgttccgc caattgaaag ctcgattcag
     1441 gcatgagacg gcacggcgtg aagctggggt tcgacgggtt gctcgtcgag caaccggaat
     1501 cggcgccgcc gcgggaccag cgcgatgtac tgctgcaacc acacgcacag ctcgaacagc
     1561 cgcatgcgcc accgcagcgc ccacggcggg cgcgggaaga ccgacccggc cagcgtggag
     1621 atgatcgcgc cgtgcacgtt gtgcggcccg cggccgtgca tgaacagttc gcggaacgaa
     1681 tggcgataat acccgcggat caaccgccag aagaccgcgg tgctgccttc gacgaagcga
     1741 acgtattccc gctgcgcggc cgcagccgtc ttcgatccgc gcagcatcgc gatcgtcaac
     1801 ttcgcggctt cgttgccgcc gaccatcgct agcgtcacgc cggtggaaaa aatcggatcg
     1861 aggaagcacc cggcgtcgcc gatcatgaaa tatccaggtc cggcgatggg cgagcaggtg
     1921 tagctgaagt cggacagcac gtcgttggtg gccggaccga cggccgcgcg catacgatgt
     1981 ctaacgaccg ggcagcgcgc gatcgcccat tgcagcagcc gatcgggcgg cacgcgcagg
     2041 tcgcgcacga accatggccg ggtgacgaaa cccacgctgg tccgccgatc ggtcaggccg
     2101 atcaaccaga accatccttc gtcggccatg acgatcgtcg gatcgccact ggcgtcgccc
     2161 ggcgggcgct cgacgtcgtc gaactgctgg aagtacgcga ccttttgcag ctcgggatcg
     2221 tcaaagttcc gccgctgctt cagatgccgg ccgacgaccg tgccgtgacc gctggcgtcc
     2281 agcagcacgc gggcggtgac cggcccgttg tcggtggtga cggcaacgtc gccgtcgctg
     2341 agccgatcga tcgcgcgcac cgcggttttt tcgaagacgg ccgcgccggc ggatcgcgcg
     2401 ttgtcgatga gcatcttatc gaagccggag cgttcgatgt tgaacacgac gtcgcccggc
     2461 agcaaaccgt cggcgaagcg aaactgcatc gtcttcaggt cgtcgcccat ggcaaattcc
     2521 gcgccgtact tcggcacgtg cggcagcttt cgcagctgct cttccagacc caattcgcgg
     2581 atcatcgcca tgttccgcgg cagtatcgat tcgccgatgt gaaagcgcgg atgcgttgcc
     2641 ctttcgaaaa ggccaacgct gaagccggcg cgagccagca cgatggccgc ggtggaaccg
     2701 gcggggccgc cgccgatgat gatgacgtca taatcttttt tcgttgtggt ggaaggcatc
     2761 atgcgaactc aactcaggcg ggcaacgccc tcgatctcta cgagcagatc ggaacggcac
     2821 aactccgcgc ttacccactc gatcgatgtc gattcaacaa atgcgctgcg cgcgaggcga
     2881 tcgaccgcct gctggtccac gcccggtggg acgtaaatgc gaatgtgctg caaggtctcg
     2941 agcggcgacg gccgcggggg cgactcgatc cgatgatcgt cgtcgcgcgc tttcgcgatc
     3001 acgatcgaga ggttccgcag cgtcagctca aactgctttt cgagatccgc gccgaaccgg
     3061 ctttcctcac cgatgatcgc ggcggtgccg gaaatgaaca gtgtggacat cggcgacatc
     3121 acccgcgtcg cccgggcgaa gcacggcggc accggcccgt agcgcggcga atagtgatac
     3181 gccggcactt gatccggatt ctcgaccgcc acgccgggcg tcgccatcgc cagagcgtga
     3241 atgaccaggt catcgccgtc atgtcccacg cccgaggcgg cgggcacgca gcggcgaatt
     3301 tcctcggccg cgccgaacca catgcgcatc gccgcaaatc gcccggcgtt gaagagcatg
     3361 tagcggtctt gcccgggatt gagcggatcg tgaatgcccg ggatgtggtt ccacatccgc
     3421 acgatgtgct ggtgcggcga ctcgcacagc gcctgccgaa cttgctcgta ggcgtcgatc
     3481 acttgttgcg agagccgctg cggatcgccg tggcggaacc cgtcaacgcg cgtcgtcacc
     3541 agggtcgcca agccacgttg cgcggccagc tccgacgccc accgaggcgg gcgcagcggg
     3601 gccgcatcgg ggacgcttgc gattggcggt tttggcttga gcgcgttcaa catcttcctg
     3661 ttgccaacga cgtccccaca gacggtgggt cgcatttgtg tagcacgggc gtctcgcccg
     3721 tgcctaagtg ctcggacacg ggagagacgc ccgtgcttcg tgaagacgac ccactatttc
     3781 cccacagcat atcggaggat catcgggcga caacgcctac gccggctttg tctgcggttt
     3841 acgttggcct agttccaggt gcgctgtggc aacgccgcct tgccaatcgg tgcagatgac
     3901 gccgaaacga tcggtgcgac cgacagcgcg cgccgcagcg ggcgtgggcg tcgcatcggg
     3961 caaatgaccg agaacgatcg ccgctgcggc gctgatgagc ggcgtcgtgc tgaacgcctc
     4021 accaaagcgc gagtaaatgc tgccgacggc gcgaacgcca tcgtcccgcg tcagcgcgtt
     4081 catctccagt ttgtcgatcc acgtgccgca accggagccg acccattggg cttcgcgcgg
     4141 cgcatcacgc agcacgctgg ccagcgtccg cggcagcgca tcaggtgtgc cgcgggcggc
     4201 ggcggcgtga cccaacttcg cgtatggttc gacaccgcgc gcgcgggcgg cgtcggcgga
     4261 ctcgatgacg aaggccaccg ctcccgatgc ggtgcggaac cccgcggtgg atgcgtaggc
     4321 gggccccaga tcgcgcgtgc ggcgcagacc gcagtgttcg tagacgcgtt cgatcagcgg
     4381 tgaatcttct tcgccggcgc cgacgatgat gcgctcggcc tgccccgccg cgatgcgcag
     4441 ccacgccagc cgcagcgcat cgagccccgc ggtgcgcgtg ccgatgatcg actggcatga
     4501 ttgcttcagg cccaacatga ggctcagttg agcggcgccg acgttgggga cgccttcggc
     4561 aaacagcacg gggttggcgg ccaacacgcc gtcgcgcacg atctgctggt aatactcgtg
     4621 gcagaaattc accgcgccat gcgtcgtgcc aagcatcgcg ccggtctcgg cgagcagcgc
     4681 cgggtcgtcg agtagccggg cgtcgccgca cgcctgctgg gcagcggcga gcatgatcgt
     4741 ggtgtatcgg ctcatgcgcc gaactcgccg ggcgttgagc aggtgctcga agtcgctgtc
     4801 ggcaagcgga ggcggcgacg aaacccacgc gggtgcggcg gcatcgtcct tcagtttcgc
     4861 cgcaaaggca tcgatcccga cggcgcccgg cagaactacg ccgatgccgg tgatccagac
     4921 ttcacgcagc gccagcttgg acgcgggcgt gtttgtatcc gcgacttcga cagccgcggg
     4981 tggcttggcg agaacaacgc atccgttcgc cccgccgaaa ccgagcgaca tgttcatcgt
     5041 gcgttggagt ggaacttttc gcgcggcgcc atgggtgacg tttagtcgct cgaactccag
     5101 ctgggccgga tcgaagtttg ccgttggtgg gatcacgccg tcgcgcaacg ccattgtaga
     5161 cattatcagt tctgcggcgc cggcggcgcc gagcgtgtga ccgaggtgcg ttttgaaaca
     5221 gacgactggc gtttgcggca agcgttcgcc cagcacctgc gacaacgccg catgttccgc
     5281 cgcgtcattg tccggcgtgc cggtggcgtg ggcggcgacg aggccaactt cggacggcgc
     5341 gacgccggcg cgcagcatcg cgacgcgcat cgcccgggcg gcgccatcgc ccttcgggtg
     5401 cgattgcgtg aggtgatgtg cgtcggccga ctcggcccaa cccgcgatga tgccgcagac
     5461 gttcgcgccg cgccgcttgg catcctcgag ccgttccagg acgagaatgg catacccttc
     5521 acccagcttc atcccccggc ggtcgcggct gaacggtcgc aacggaccat cggcgacgag
     5581 ccgcaatgaa ttgaagccgg cccagacata ttcgctgacg gtgtcgtatc ccccggcgac
     5641 gacgagatcg gccgccccgg tttgcagcag cgtggcagcc agcgcgacgc tgcctagtcc
     5701 ggacgagcag gccgagcacg atgtggtggc ggcccctgcg aaatccagat cgtccagcgc
     5761 caatcgcagc acgtcaccgg ccaggaacgt gccaagctcg ccggcatctt cgctgcgata
     5821 gaaccgcccg ccggcgcgca tgccgtgtag cgtggtgccg atggcgacgg cgcggcgcgc
     5881 tggtggaatt gcctgccgcg cagacggaga agataggccc gcgtcgcgca aggcggactc
     5941 gatcgtccag cgcaggtatc gggtctcgcg cggcaactgc ggttgataat cccgcggcag
     6001 atcgaccgcc tgatgaccgg cggcgccgac cggcagcggg gattcgatgt ccggcatcgg
     6061 cgcgggctgc gcatcgccgc gcaggacggc ttgccatgtg tcctcggccg tcgcccccag
     6121 cgcggtcgtc agaccgacgc cggtgatggc gatcgccgcc gcgctatttg catcgctcct
     6181 caactgcacg acgtttctcc tcgctcgatt cgtcaacaaa cgcgcggtcg agcacgagcc
     6241 attgctgcgg gtgttgccgc acgaaagact caaaggtccg cgctaacgaa agcagtgccg
     6301 ggtcggtctc atctgatgcg ctattctcgc cctcctggac atacaccggc tcgccaagat
     6361 gaatgcgaaa tcgaccgcgc ccaccgcgta aaacgatcac cggcacgagc ggactgccgg
     6421 tcaagcgggc gagtttcacc ggaccgattg gcatccgcaa gcggccgtgt aaaaagggta
     6481 caacggcggc tcgctcgccc ggcatcgcgc gatcgccttg catcaccacc gcctcgttgg
     6541 ccagcagcgc atcgcgcagg gccatcagcg cgggcagccc gccgtcgatg gccgcctcgc
     6601 gcacctccaa ctgctttcgc acctgggtgc ggatgcgttc gaaaccggcg aaatcgtccc
     6661 gtttgaagac gacatgcacg cgcgactcga cccttcgaag cgccgcgagc ccgacttcaa
     6721 acgccccgac gtgcgctgtc acgaggatca cccctcgttt gtgcttacgc gcctcgagat
     6781 acgccggctc accatcgacg ccttcgatgc gactcaagat ctcggcggtc gacgcgcgac
     6841 cgctcgcggc gagttccacc acaaaatcgt aaaagttgcg gatgaccccg cgcgcgaagt
     6901 cgcgctgctc ggcggccggc aatgggcgac cgaagatgca cgcggcgttg agccgcaagt
     6961 tttccgcgac tgagcgcgac gaccagagcg tgatcggcgg caccagcggc cggatccacc
     7021 gcatcaccca cccggcgtgc tcgccgagga agaaaaacag cgccaggcac gcccggaccc
     7081 acgcgggttg cggcggtggc ttcacatcat cggcgtcgcc gcgcgcgttc atcgcggcgg
     7141 cgtctccggc tggcccagcg gatggacgat cttcatgttc gccggcaagt cgagccgcac
     7201 ggcgtcgtcg gtcaggccaa cgtcggtttt gatgttcgag aagcggatga gcgtgcgctc
     7261 gccgtcgggg tcgaccatct ccagcgcca
//
LOCUS       DATIMH010000989         1658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2835474, whole genome shotgun sequence.
ACCESSION   DATIMH010000989 DATIMH010000000
VERSION     DATIMH010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835474"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..916)
                     /locus_tag="VLI90_17175"
     CDS             complement(329..916)
                     /locus_tag="VLI90_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15997.1"
                     /translation="MDLSKLPKLSNTQAEQPPASAPVASDDPTPTRPVEYRGRPSINE
                     PELGADIWVNAIIGLLLIGMGFTFAKFLSAKLTGQPFHTNVNWTTGPNAGSEVAYFDL
                     QGYTAWTDMGMFLFGVVLLLEAAAKTALVLRPGAVSRAILLLALVLTAATTALNIYVC
                     FKFLQAGSLPLLSGLAVAFGGWILADERRMLRHQT"
     gene            1069..>1658
                     /locus_tag="VLI90_17180"
     CDS             1069..>1658
                     /locus_tag="VLI90_17180"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV15998.1"
                     /translation="MPDALEQIEALAKLAGQELTTVKGPADLEAFRIKYLGTKGQIKD
                     LMKLIGTAPVDQKPLIGQRVNAVKQDVTTAFEAKKAELASSGAGSKDGIDVTEPGRPL
                     SIGNRHILMKVVDELVELFGRMGFSVATGPEVEDEFHNFIALNIPKQHPARDPLDNFY
                     LAGSAGSEEQIKLLRTQTSTVQIRVMEQQRPPIRVV"
BASE COUNT          335 a          444 c          567 g          312 t
ORIGIN      
        1 gagacaggcg gtgggcgcgg gcggttgggt tggctggatc gccgtgctcg gttacctcgg
       61 tttgtgcgtg atcctgttcg tcggccgggc gtaaaaaaaa tatgaattgc cgcgactcgg
      121 gcaagtgact tggatgaatg aagtgcctag agttttgtgg ctgtggagtg aaaacgaaac
      181 aacaacgaga ggctgaccta aggaagtagt ttgaacgaaa ccggactgta gcctctcaca
      241 agctcctacc ctcccctgag ccccggacgt cttccccgcg tccggggttt tttatgcgct
      301 cgcaaaaacg tgaggagcga atctgcgatt acgtttgatg cctcagcatc cggcgttcat
      361 ccgcgaggat ccagccgccg aaggcgacag cgaggccgct tagcagcggc agcgagccag
      421 cctggagaaa tttgaaacag acgtagatgt tgagtgccgt cgtcgcggcg gtgagtacga
      481 gggcgagcag caggatcgcg cggctgacag cgcctggtcg caagacgagg gcggttttgg
      541 cggcggcttc gagcagcagc acgacgccga acaggaacat gcccatgtcg gtccacgcgg
      601 tgtagccttg cagatcgaag tacgcgactt cgctgccggc gttgggaccg gtggtccagt
      661 tgacgttcgt gtggaagggt tggccggtga gcttggcgga gaggaatttc gcgaacgtga
      721 agcccatgcc gatcagcagc aggccgatga tggcgttgac ccagatgtcg gcgccgagtt
      781 cgggttcgtt gatggatggc cggccgcggt actcgacggg ccgcgttggg gtgggatcat
      841 cggatgcgac tggcgcggag gcggggggtt gttccgcttg ggtgtttgag agcttcggta
      901 gcttcgacag gtccatgctg gtcctcccgc gcgcgacgca cagtgaaacg acgcggttgt
      961 tttcatccgg gcgccgttct catcgtcgcg tgtcttgcat ctggggcgtc gcccgtaaga
     1021 atgctatccg atttagctgc tcgatggaca aagaaggaga cgccgatcgt gcctgatgcg
     1081 ctggaacaga ttgaagccct tgccaagctg gccgggcagg aactgacgac ggtcaaaggc
     1141 ccggcagatc tcgaagcctt ccgcatcaag tacctcggca ccaaggggca aatcaaagac
     1201 ctcatgaagc tgatcggcac cgccccggtg gatcagaagc cgctcattgg ccagcgcgtg
     1261 aacgcggtca aacaggatgt gacgacggcg ttcgaagcga agaaggcgga gctggccagc
     1321 agcggggcgg ggtcgaaaga tggcatcgac gtgaccgagc cgggtcggcc gttgtcgatc
     1381 ggcaatcggc acatcctgat gaaggtggtc gatgagctgg tcgagctgtt tggtcgaatg
     1441 ggtttctctg tcgccactgg cccggaagtt gaagacgagt tccacaactt catcgcgttg
     1501 aacatcccga agcagcaccc ggcgcgcgat ccgctcgaca acttctatct cgccggaagc
     1561 gccgggagcg aagagcagat caagctgctg cggacgcaaa ccagcacggt gcaaatccgc
     1621 gtgatggagc agcagcggcc gcccattcgc gtcgtcat
//
LOCUS       DATIMH010000990         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1684428, whole genome shotgun sequence.
ACCESSION   DATIMH010000990 DATIMH010000000
VERSION     DATIMH010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684428"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /locus_tag="VLI90_17185"
     CDS             <1..1852
                     /locus_tag="VLI90_17185"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HSV15999.1"
                     /translation="YLTNKRMPDAVDHSVSQFGVLGTWACQQAGVEVPSRYWEMVEKR
                     WIADQQKDGGWIYGAALDPKWPAHSHEQASMTAAGVATLFITQEFVHANDGIKCVGNI
                     HNNAIDAGLKWMADNTKDWIPDDHWGSAKYMYWLPGYTLYGVERIGVASGLKYIGSVD
                     WYQFGADWCVKLQAPWGAWGDEQDVPNTAFCMLFLSRGRAPVMISKLSYDNAEGDEKS
                     QPGRWNQRPRDVANFVHWMARQTERDLNWQITNLNVPADELNDSPILYIAGSQPITLT
                     ADQQAKLKTFIEQGGMILCNADCGNGGFSSSILKLGKQMFPQYEFRDLPADHPIFTNE
                     QYSPKKWKNKVTLRGLSNGVRELIVTLNGDAGRSWQQGSEAGREDAYQPAANIVLYAA
                     GKQNLRVKGSTYLVTADSNIRAERSAKLARLRYDGAWDPEPGGWRRLAAMMHNQCKFD
                     LKVETVDLGKAPLDGFSVAHLTGTNDFKFSAAQIDALKIFIDAGGTLLVDSAGGGAAF
                     AESAEALLNQMYPGGLKDPLPPDDAVFTAGTASGKIQYRDYARAMLGTLRTPRLKAIT
                     VNGRHAVYYSREDLSGGLVGEPVDGIVGYEPDTAAAIVSGVIVQAGNAAH"
BASE COUNT          412 a          586 c          677 g          329 t
ORIGIN      
        1 gtatctcacc aacaagcgca tgcccgatgc ggtggatcac agcgtcagcc aattcggcgt
       61 gctcgggacg tgggcctgcc aacaggccgg tgtcgaggtg ccgagccggt attgggagat
      121 ggtcgagaag cgctggatcg ccgatcaaca gaaggacggc gggtggattt acggcgcagc
      181 gctcgatccg aaatggcccg cgcacagcca cgagcaggcg tcgatgaccg ccgcgggggt
      241 ggccacgctg ttcatcacgc aggagttcgt tcacgccaac gatggaatta aatgcgttgg
      301 caacattcac aataacgcga tcgacgccgg cctgaagtgg atggccgaca acaccaagga
      361 ctggattccc gacgaccact ggggctcggc aaaatacatg tactggctgc cgggctacac
      421 gctgtacggc gtcgagcgca tcggcgtcgc gagtggactc aagtacatcg gctcggtcga
      481 ttggtatcag ttcggcgccg actggtgcgt gaagttgcaa gccccctggg gcgcgtgggg
      541 cgatgagcag gacgtgccga ataccgcgtt ctgcatgctg ttcctgtcgc gcgggcgcgc
      601 gccggtgatg atcagcaagt tgtcatatga caacgccgag ggcgacgaaa aaagccagcc
      661 cggccggtgg aaccagcggc cgcgcgacgt ggcgaacttc gtgcactgga tggcccggca
      721 aaccgagcgc gacctgaact ggcaaatcac caacctgaac gtgcccgccg atgaattgaa
      781 cgattcgccg atcctgtaca tcgccggcag ccaaccgatc acgctgacgg cggaccagca
      841 ggcgaagctg aagacgttca tcgagcaagg ggggatgatt ttgtgcaacg ccgactgcgg
      901 caacggagga ttctcgtcat cgatcctcaa gctcggcaag cagatgtttc cacagtacga
      961 atttcgcgac ctgccggcgg atcacccgat tttcaccaac gagcaatact cgccgaagaa
     1021 gtggaagaac aaggtgacgc tgcgcggcct gagcaacggc gtgcgcgagc tgatcgtcac
     1081 gctgaacggc gatgccgggc ggtcgtggca acaaggctcc gaagccggcc gcgaggacgc
     1141 gtatcaaccg gcggcgaata tcgtgctgta cgcggcgggc aagcagaacc tgcgggtgaa
     1201 gggatcgacg tacctcgtga cggcggattc aaacatccgc gcggagcgct cggcgaaatt
     1261 ggcgcgcctg cgctacgacg gcgcgtggga ccccgagccc ggcgggtggc gccgcctggc
     1321 cgccatgatg cacaaccagt gcaagttcga cctgaaggtc gagacggtcg atctgggcaa
     1381 agcgccgctc gatggcttca gcgtcgcgca cctgacggga acgaatgatt tcaaattcag
     1441 cgcggcgcaa atcgacgcgc tgaagatttt catcgacgcc ggcggtacgc tgctggtcga
     1501 ttccgccggc ggcggggcgg cgtttgccga gtcggccgaa gcgttgttga accagatgta
     1561 ccctggcgga ttgaaggatc cgctgccgcc ggacgatgct gtcttcaccg ccggcaccgc
     1621 gtcggggaag atccaatatc gcgattacgc ccgggcgatg ctcggcaccc tgcgcacgcc
     1681 gcggttgaag gcgatcaccg tgaatggccg gcacgcggtc tattacagtc gcgaagatct
     1741 gagcggcggg ttggtcggcg agccggtgga tggcatcgtc ggctacgaac ccgacaccgc
     1801 cgcggcgatc gtgtccggcg tgatcgtgca ggcgggcaat gcggcgcatt gagccgcttg
     1861 cggcagagcg tctgctcccg tcttacctcc tcgcccggta ctccgagaga gggctggggt
     1921 gagggtgatt tcgggtgaga aaatccgaat gtcgaagtcg aatccccgaa tcatatacgg
     1981 atgacgaatg acgaatggcg gaca
//
LOCUS       DATIMH010000991         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2836064, whole genome shotgun sequence.
ACCESSION   DATIMH010000991 DATIMH010000000
VERSION     DATIMH010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2836064"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VLI90_17190"
     CDS             <1..386
                     /locus_tag="VLI90_17190"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HSV16000.1"
                     /translation="RAGTMSLCIPYNVIEPIMSVLAAQNWFSYQRKGNQDEHIKKLTK
                     NVQNAPVEARAFLVQTSMTMNELISLQVGDIITTEKAASRDVLIQVEGKNKFLGQVGQ
                     LRGSKAVRITRACQEAAEPTPGAAR"
     gene            383..1171
                     /locus_tag="VLI90_17195"
     CDS             383..1171
                     /locus_tag="VLI90_17195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSV16001.1"
                     /translation="MSRAAAVGNASAAPRRASAPAASTSAPNGYFEQSALPLASLVFL
                     APLIVLYELGTRWYASDPVSHVEQRIIAFSLMQQFFSLFGATGKYLPAAAVVAILLAW
                     HIARNDAWTTHIGHLVGMFFESAVYAIPLRALAMAFAHYLPLYSPPDKSRALLVLSVG
                     AGIYEEMVFRLIAFTVMSVLFIDVLKMEKIRAFLLMVLVSSLAFSAYHYLGSEAFEWR
                     SFAFRTLAGIYFGIIFVWRGFGITAGAHAFYDISTFALRMAAHV"
     gene            1408..1962
                     /locus_tag="VLI90_17200"
     CDS             1408..1962
                     /locus_tag="VLI90_17200"
                     /inference="COORDINATES: protein motif:HMM:NF022389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2617 family protein"
                     /protein_id="HSV16002.1"
                     /translation="MSNRTKQRRSGGLTLCLYQRQLHPEFFKILATEAVSRRAYEADV
                     WLVEGGHVISFSAGKTTMAEVIVTNPTQLNDRGLIQTIPCRGERYHETTIGGNVRYMI
                     STQEEQLTQTLYDATKHEISNYAAKRELMMAETPTTDQTSGSLSVLDIERRSHELLVQ
                     SFHLFDETMMVIKTQAIIEVIKNA"
     gene            complement(2064..2261)
                     /locus_tag="VLI90_17205"
     CDS             complement(2064..2261)
                     /locus_tag="VLI90_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16003.1"
                     /translation="MVGLGRITLVMFLRVASMAAGSTDDAAADGEPSRKIFPIGNAAR
                     ESKNAARLPILKQILLLRPQD"
     gene            complement(2301..2819)
                     /locus_tag="VLI90_17210"
     CDS             complement(2301..2819)
                     /locus_tag="VLI90_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV16004.1"
                     /translation="MLAPAARGALSASASLSTSQASAPFTYTIKLTNTGDLNIASFWF
                     AWTDVPAAYDFLPTAPTNVVAPSGWFAPVVHAFGPPNGYSLEFYVFGNGITPGNTGTF
                     QFTSNDTPAQLGGPAWFGGHTGTDSVIYQGLPQSATSAHIAVAVPEPASLTLGGVGGM
                     LLLMRRRRTVGR"
     gene            complement(2924..>4036)
                     /locus_tag="VLI90_17215"
     CDS             complement(2924..>4036)
                     /locus_tag="VLI90_17215"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSV16005.1"
                     /translation="VTVNSVTYTDPTHITLNLTLAVNAAAGLRSITVTNPDGQSISSA
                     SGILTITPTAPAAPDLQTASDAGTSSTDNITNVTNPTFDVAAVQAGATVDLLRDGVVV
                     ATTTNAAAGTVTLQDPGPAAAGSRSYTTRQTVSGVISAASTALVVTIDTTAPQLVGTP
                     GFQFNVAPQSISYAFSEDVGASLTPHDFLVEQLPTGGATVADADKSVSGTSSIVLGFT
                     SVLASGNYRVTIAASDVTDTAGNALSAGSTFDFFIYAGDVNHDRWVDVGDLGLLATNY
                     GTSSGATWGQGDFNYDGAVDVADLGQLAGNYGTTLAQPTGSAVTAASAMMASPAAAPA
                     DPFWKHTGNLWGDLPIKAAPEPLGRIAECKGCMKSI"
BASE COUNT          764 a         1270 c         1235 g          767 t
ORIGIN      
        1 atcgcgccgg cacgatgagc ttgtgcattc cgtacaacgt catcgaaccc atcatgagcg
       61 tgctggcggc ccagaattgg ttcagctatc agcgcaaggg gaatcaggac gagcacatca
      121 agaagctgac caagaacgtg cagaacgcgc cggtggaagc gcgggcgttt ttggtgcaga
      181 cgtcgatgac gatgaacgaa ctgatctcgc tgcaggtggg cgacattatc acgaccgaga
      241 aagccgcatc gcgcgatgtg ctgatccagg tggaagggaa aaataaattt ctcgggcagg
      301 tcggccagct gcgcggcagc aaagccgtgc ggatcacgcg cgcctgccaa gaagcggccg
      361 aacccacgcc gggggccgca cgatgagccg ggcggctgcc gttggaaatg caagcgctgc
      421 gccgcggcga gcatccgcgc ccgcggcgtc gacttccgcg cccaatggtt acttcgaaca
      481 atccgccctg ccgctggcga gcctggtctt cctcgccccg ctgatcgtgc tgtacgaact
      541 gggcacgcgc tggtacgcat ccgaccccgt cagccacgtc gagcagcgca tcatcgcctt
      601 cagcttgatg cagcagttct tctcgctgtt cggcgccacc ggcaaatatt tgcccgccgc
      661 ggccgtggtg gcaatcctgc tcgcctggca catcgcgcgg aacgacgcgt ggacgacgca
      721 catcgggcac ctcgtcggca tgttcttcga gagcgctgtt tacgccatcc cccttcgggc
      781 gttggccatg gcgttcgcgc attacctgcc gctgtacagt ccgccggaca agtctcgcgc
      841 gctgctggtc ttgtcggtcg gtgctggcat ctatgaagag atggtgttcc ggttgatcgc
      901 cttcaccgtc atgagcgtgt tgttcatcga cgtgctgaag atggaaaaga tccgggcgtt
      961 cctactaatg gtgctggtgt cgtcgcttgc gttctccgcc taccattact tggggtcgga
     1021 agcgttcgaa tggcggagtt ttgcctttcg gacgctggcg ggaatttatt ttgggatcat
     1081 tttcgtctgg cgtggttttg ggataaccgc aggcgcacac gcattttacg acatatcaac
     1141 cttcgccctt cgcatggcag cgcatgtctg atgccgatta ttttgtaacg ctcgccacgt
     1201 tcatctctat ataatccgtc catgttgagt catccgcatc gggcggatgg cgttaaaggc
     1261 gacgcccggc tgggtttatg gatgaacccg accggtagca cccgaacttc agggtgcggg
     1321 tgtcgtattc gattcagaag gacattgtgc cttctgtaac aatgtagaga gcggcgcctc
     1381 tctgctagaa agtggcacgg acgcgttatg agcaaccgaa ccaagcaacg tcgttccggc
     1441 ggattgaccc tgtgtctcta ccagcggcaa ctccacccgg agttcttcaa gatcctcgcc
     1501 accgaggcgg tcagccgccg cgcctatgaa gccgatgtct ggctcgtcga aggcgggcac
     1561 gtgatcagtt tctcggctgg caaaaccacg atggcggaag tcatcgtcac caaccccacg
     1621 cagctgaacg accgcggttt gatccagacg atcccctgcc gcggtgaacg ttatcacgag
     1681 accacgatcg gcggaaacgt ccgctacatg atcagcacgc aggaagagca gctgacgcag
     1741 acgctgtacg atgccaccaa gcacgaaatc agcaactacg cagccaagcg cgaactgatg
     1801 atggccgaga cgccgacgac cgaccagacc agcggctcgc tgagcgtgct ggacatcgaa
     1861 cgccgcagcc acgaactgct ggtgcagagc ttccacctgt tcgatgagac catgatggtc
     1921 atcaagacgc aggcgatcat cgaagtgatc aagaacgcgt aagcgattcc gcgaatcacg
     1981 aaaaaaactc ccggcccggt gacgagaagt caccgggccg gttttgtttg acggaccacc
     2041 gccggtgcgc gtgcggacac ctgttaatcc tggggcctca atagcagtat ctgttttaag
     2101 atggggaggc gcgccgcatt tttcgattcg cgcgccgcat ttccgattgg gaaaattttt
     2161 cgcgatggct cgccgtcggc ggccgcatca tcggtcgaac ccgcggccat cgacgcgacg
     2221 cgcaaaaaca taaccagcgt gatgcggcca aggcccacca taactgccgg cgatcatatt
     2281 atcgcaggat caaatatgcc tcaccggccg acggtgcgtc gccggcgcat gagcagcagc
     2341 attccgccca caccacccag cgtcagtgac gccggttccg gcacggccac ggcgatgtgc
     2401 gccgacgtcg ccgactgcgg caggccctga tagatcaccg agtcggtccc cgtatgtccg
     2461 ccaaaccacg cgggcccccc cagctgcgct ggtgtgtcgt tcgacgtgaa ctgaaacgtc
     2521 ccggtgttac ccggtgtgat gccgttgccg aagacgtaga actccaggct gtatccgttg
     2581 gggggaccaa acgcatgaac gaccggggcg aaccaccccg acggcgccac cacgttggtc
     2641 ggcgccgtgg gcagaaaatc gtatgccgcg ggtacatcgg tccatgcgaa ccagaagctc
     2701 gcgatgttca gatcgccggt gttcgtcagc ttgatggtgt atgtgaacgg cgcgctggcc
     2761 tggctggtcg ataagctagc cgacgccgac aacgccccgc gagccgcggg cgccaacatc
     2821 aaaccgacca agccaaagac gcaactcaga cgtgcccgaa gtgaagccct catcatctac
     2881 ctctccctgt gtggtttggc ggaccatacc actcaggaag cgattagata gatttcatgc
     2941 accctttgca ttctgcaatt cgccccagag gctccggtgc cgctttgatc ggcagatcgc
     3001 cccacaggtt gccggtgtgc ttccaaaacg gatcagcagg ggcagccgcg ggcgatgcca
     3061 tcatggcgct tgcggcggtg accgccgatc ccgtcggttg cgccagcgtc gtgccgtagt
     3121 tgccggccag ttgccccagg tccgcaacgt ccaccgcgcc gtcgtagttg aagtcgccct
     3181 gtccccaggt cgccccgctg ctcgtgccgt agttggtcgc cagcaagccg aggtcgccca
     3241 cgtctaccca gcgatcgtga ttgacgtcgc cggcgtaaat gaagaagtcg aaggtgctac
     3301 cggcggatag cgcgttgccc gcggtgtccg tgacatcgct cgccgcaatg gtcacgcgat
     3361 agttcccgct cgccagcacc gacgtaaaac cgagcacgat gctgctcgtg ccgctcacgc
     3421 tcttgtccgc gtccgccacc gtcgccccgc cggttggcaa ctgctccacc aggaaatcgt
     3481 gcggcgtcag gctcgcgccc acgtcttcgc tgaaggcata gctgatcgac tgcggcgcca
     3541 cgttgaactg aaaacccggc gtgccgacca gctgcggcgc cgtcgtgtcg atcgtgacga
     3601 ccaacgccgt cgatgcggcg ctgatcacgc ctgaaaccgt ctggcgcgtc gtgtaactgc
     3661 gcgaccccgc ggccgccgga ccggggtctt gtaacgtcac ggtgccggcg gcggcgttgg
     3721 tcgtggtcgc cacgaccaca ccgtcgcgca gcagatcaac cgtcgccccc gcctgcacgg
     3781 cggcgacgtc gaacgtcggg ttcgtcacgt tggtgatgtt gtcggtgctg gacgtgccgg
     3841 catcgctggc ggtctgtaaa tccggggcgg ccggcgcggt tggagtgatc gtcaggatgc
     3901 cgctcgcgct gctgatggat tgaccgtccg ggttggtcac cgtgatcgac cgcaaccccg
     3961 cggcagcgtt gacggccagc gtcaggttca gcgtgatgtg cgtcgggtcg gtgtacgtca
     4021 cgctgttgac cgtcac
//
LOCUS       DATIMH010000992         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3703881, whole genome shotgun sequence.
ACCESSION   DATIMH010000992 DATIMH010000000
VERSION     DATIMH010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3703881"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2792
                     /gene="gcvP"
                     /locus_tag="VLI90_17220"
     CDS             <1..2792
                     /gene="gcvP"
                     /locus_tag="VLI90_17220"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HSV16006.1"
                     /translation="GYDSLDDLAEATVPAAIRMKRPLQIGEPRGEFELLQELKSIASK
                     NKINRSLIGMGFYDTITPPVILRNILENPGWYTQYTPYQAEIAQGRLEALLNFQTMVA
                     DLTGLPLANASLLDESTAAAEAMAMCRSMSEGKRNAFLVAQDCHPQTIAVVQARARAL
                     GIECIVGDVERADFSKGNLCGVLIQYPTSDGRVVDYTGIVNKAHGSAVGPVAVVIAAD
                     LLALTLLKTPGEFGADIAIGSTQRFGVPLGFGGPHAAYMATKTEFARKMPGRLVGVSK
                     DAQGDPAYRLAIQTREQHIRREKATSNICTAQVLLAIIAGMYAAYHGPHGLKRIAQRV
                     HGLTKALATGLQRVGHDVGAAPFFDTLKVRLNGLSADAVIRAAESSGYNLRRYDDGCV
                     GVSLDETTTRGEVEAIIGAFAGGSTFRVSVDELSTQAPPLPSELLRTSEYLTHPVFNR
                     YHSETEMLRYIFRLMSRDLSLAQSMIPLGSCTMKLNATSEMIPVTWPEFGRVHPFAPA
                     DQTHGYQQMFEQLERWLAEITGFSAVSLQPNAGSQGEYAGLLVIRAYHESRGQRHRTV
                     CLIPTSAHGTNPASAVVAGLKVVAVACDEKGNVDLDDLAKKAKEHEQNLAALMVTYPS
                     THGVFEARIKDICDIVHRHGGQVYMDGANMNAQVGLCRPGDIGADVCHLNLHKTFCIP
                     HGGGGPGMGPIAVASQLAPFLPGHPVTKLGGGQAIGPISESPYGSASILTISWVYIAL
                     MGGVGLTRATQVAILNANYMAKKLQGHYDQLYTGQNGRVAHEFILDCRPFQQSAGVTV
                     EDIAKRLMDYGFHAPTMSWPVPGTLMIEPTESESKAELDRFCEALIKIRDEIRAIEEG
                     KTDRADNALKGAPHTAQALISDSWSRKYAREQAAYPAPWLREYKFWPAVGRIDNPYGD
                     RHLVCTCPPIDDQVE"
     gene            2946..3167
                     /locus_tag="VLI90_17225"
     CDS             2946..3167
                     /locus_tag="VLI90_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSV16007.1"
                     /translation="MQNQFSAVYQKHDQWWIAWVAELPGANTQGATLEEARENLKEAV
                     ALVLEGNRELAERDLADKQVIREDLVVTT"
     gene            complement(3415..3663)
                     /locus_tag="VLI90_17230"
     CDS             complement(3415..3663)
                     /locus_tag="VLI90_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16008.1"
                     /translation="MSSLSPLGFHVVRRSIEEPTRSERVIIQRPLQRVLAFTRRSIDD
                     VVNCPIVKNEVLGYYKLHKWVERGCEITDMNRWWSGGR"
     gene            complement(3788..>4445)
                     /locus_tag="VLI90_17235"
     CDS             complement(3788..>4445)
                     /locus_tag="VLI90_17235"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HSV16009.1"
                     /translation="TDHPRRLNVPGEDLPHVSHYFQDPHTYFRKRLLIVGGKNSAVEA
                     ALRCYHAGADVSISYRRDQLNQSSIKYWLLPEINGLIEANKIHGYFGTEPVEITLSHV
                     RFCRSRYCQIKEHRKYDPQPFDFVLLLIGYEADQTLLRIVGVELHGDCRTPVFNQNTM
                     QTNVPGIYVAGCAIGGTQDKYAIFLENCHVHVPRIIGDITGQRIEIPISTPEFSRPET
                     "
BASE COUNT          863 a         1426 c         1386 g          770 t
ORIGIN      
        1 tcggttacga ctcgctggac gatttggccg aggcgaccgt tcccgcggcc atccgaatga
       61 agcgcccgct tcaaatcggc gaaccccgcg gcgagttcga acttctgcag gaactgaaat
      121 cgatcgccag caaaaacaag atcaaccgct cgctgatcgg catgggcttt tacgacacga
      181 tcacgccgcc ggtgattttg cggaacatcc tggagaaccc ggggtggtac acgcagtaca
      241 cgccgtatca ggcggagatc gcccagggcc ggctcgaggc gctgctgaac ttccagacga
      301 tggtggctga tctgacaggc ctgcccctgg cgaacgcgtc gctgctggac gagagcaccg
      361 ccgccgccga ggcgatggcg atgtgccggt cgatgagcga aggcaagcgc aacgcgtttc
      421 tagtcgcgca ggattgccac ccccagacga tcgcggtggt gcaggcgcga gcccgcgcgc
      481 tgggcatcga gtgcatcgtc ggtgatgtcg agcgcgccga cttctcaaaa ggaaatctct
      541 gcggcgtctt aattcagtat cccacctcgg atggccgcgt cgtcgattac acgggaatcg
      601 taaacaaagc ccacggctcc gccgtgggac cggttgccgt cgtcatcgcc gccgacctcc
      661 tcgccctcac gctcctcaaa actcccggcg aattcggcgc cgacatcgcc atcggttcca
      721 cccagcgctt cggcgtcccg ctcggtttcg gcggtcccca cgccgcatac atggcgacca
      781 aaaccgaatt cgcccgcaag atgccgggcc ggctggtcgg tgtctccaaa gacgcgcagg
      841 gcgatcccgc gtatcgcctg gcgatccaga cgcgcgagca gcacatccgc cgcgagaagg
      901 cgacgagcaa catctgcacc gcgcaggtgc tgctggcgat catcgccggc atgtacgccg
      961 cgtatcacgg gccgcacggg ctcaagcgaa tcgcgcagcg cgtgcacggg ctgaccaagg
     1021 cgctggcgac gggattgcag cgcgtcgggc atgatgtggg cgcggcgccg ttcttcgaca
     1081 cgttgaaggt gcgcctgaac ggcctcagcg ccgacgccgt cattcgggcc gccgaatcca
     1141 gcggctacaa cctgcgccgc tacgacgacg gctgcgtcgg cgtctcgctg gacgaaacca
     1201 cgacgcgcgg agaagtcgag gcgatcatcg gcgcgttcgc cggcggatcg acgtttcgcg
     1261 tttcggtcga tgaactatcg acgcaggcgc cgccgctgcc aagcgaactg cttcgcacga
     1321 gcgaatacct cacgcacccg gtcttcaatc gctatcacag cgagacggag atgctgcgct
     1381 acatcttccg cctgatgagc cgcgatttgt cgctggcgca gagcatgatc ccgctgggtt
     1441 cgtgcacgat gaagctgaac gcgacgagcg agatgatccc ggtgacgtgg ccggagtttg
     1501 gccgcgttca cccgttcgcg ccggcggatc agacgcacgg ctatcagcag atgttcgagc
     1561 agttggagcg ctggctcgcc gaaatcaccg gattctcagc cgtttccctg caacccaacg
     1621 ccggcagcca gggggaatat gcggggctgc tggtcatccg ggcctatcac gaatcgcgcg
     1681 gccaacgtca tcgcaccgtc tgcctcattc ccaccagcgc ccacggcacc aaccccgcca
     1741 gcgcggtggt cgccggcctg aaagtcgtcg cggtcgcctg cgatgaaaag ggtaacgtcg
     1801 atctcgacga cctggcgaag aaggcgaagg agcacgagca gaatctcgcc gcgctgatgg
     1861 tcacgtaccc cagcacgcac ggcgtcttcg aggcgcgcat caaagacatc tgcgacatcg
     1921 tccaccgcca cggcgggcag gtatacatgg acggcgcgaa catgaacgcc caagtcggcc
     1981 tctgccgtcc cggcgacatc ggcgccgacg tctgtcacct gaacctgcac aaaaccttct
     2041 gcatccccca cggcggcggc gggcccggca tggggccgat cgcggtcgct tcgcaactcg
     2101 cgccgttcct gccgggtcac cccgtcacga aactcggcgg cggccaggcg atcggcccca
     2161 tctccgaatc gccctacggc tcagccagca tcctcaccat cagctgggtc tacatcgcgc
     2221 tgatgggcgg cgtcggcctc acgcgcgcaa cgcaggtcgc catcctcaac gcgaactaca
     2281 tggcgaagaa actgcagggc cactacgacc agctctacac cggccaaaat ggtcgcgtcg
     2341 cgcacgagtt cattttggat tgccgcccgt tccagcagag cgctggcgtc acggtggaag
     2401 atatcgctaa gcggctgatg gactacggct tccacgcccc gaccatgagc tggccggtcc
     2461 ccggcacact gatgatcgag cccaccgaga gcgaatcgaa ggcggagctg gaccgcttct
     2521 gcgaagcgct gatcaagatc cgcgacgaga ttcgcgcgat tgaagaagga aagacggatc
     2581 gcgcggacaa cgcgctcaag ggcgcgccgc acacggcgca ggcgctgatc agcgacagct
     2641 ggtcgcgcaa gtacgcgcgc gaacaagccg cctatcccgc gccgtggcta agagagtaca
     2701 agttctggcc cgcggtgggg cggatcgaca atccgtatgg cgatcgccac ttggtatgca
     2761 cctgcccgcc gatcgatgat caggtggagt gatcgacaca acaaaagccc acggctctgc
     2821 cgttcgacga aatctcgaaa taagccccac ggcaaagcct tcgtctcttc ttagcttcca
     2881 tagacccact cgatcgcggc gcctcgtaag ccttttcgtg cgcttgtgcg tataatcttt
     2941 gcatcatgca aaaccagttc agcgcggtgt accagaagca tgaccagtgg tggatcgcgt
     3001 gggtggcgga attgcctggc gccaacacgc aaggggcgac gctcgaggaa gcgcgggaga
     3061 atctcaagga agctgtggca ctcgtgctgg aaggcaatcg tgaactcgcg gagcgcgatc
     3121 tggccgacaa gcaggtgatt cgggaagacc tcgtggtcac gacgtgattt ctcgccccgc
     3181 acgcgaatga aacgccgcga cttgattcga catctcgaga cgaacggctg cggactgctg
     3241 cgagagagca gccggcatag cttctatttc aatcccgtca atcatcagac gtcgacggtc
     3301 cctcgccgca atgaaatcga cgagtttttg agccggaaga tttgcaagga cctcggcatc
     3361 gcctcaccga agcaaagcag gagagcgccg gtagcaatag gcgacgcgca ttcgctatcg
     3421 cccgccgctc caccagcggt tcatgtcggt gatctcgcag ccgcgttcca cccacttgtg
     3481 cagcttgtaa tatccgagca cctcgttctt cacgatcggg caattgacga cgtcatcgat
     3541 gctgcggcgg gtgaaggcaa gaacgcgctg cagcgggcgc tggatgatca cgcgttcgct
     3601 gcgcgtgggt tcctcgatcg agcgacggac gacgtggaag ccgagcgggg agaggctgct
     3661 catcgcactt gaattatatc ccgcggaggc tcgattttcg cgtgagaact gaactgggct
     3721 aaccgcttca gcggttagtg tgcgtcgaga ctcgaaaaca taaccgctga agcggttcgt
     3781 ccggtggtca ggtttccgga cgcgaaaact ccggcgtgct gatggggatc tcgatgcgct
     3841 gccccgtgat gtccccgatg atccgcggga cgtgcacgtg gcagttctcc aggaagatcg
     3901 cgtacttgtc ctgcgtgccg ccaatcgcgc agccggcgac gtagatgccc ggcacgttcg
     3961 tctgcatcgt gttctggttg aagaccggcg tgcggcagtc gccgtggagt tcgacgccga
     4021 cgatgcgcag cagcgtctgg tctgcctcgt agccgatcag cagcagcacg aagtcgaacg
     4081 gctgcgggtc gtacttgcgg tgctccttga tctggcaata gcgcgatctg cagaaccgca
     4141 cgtgcgacag cgtgatctcc accggctcgg tgccgaagta gccgtggatc ttgttcgctt
     4201 cgatcaggcc gttgatctcc ggcagcagcc aatacttgat gctcgactgg ttcagttgat
     4261 cgcggcggta gctgatcgac acatccgccc ccgcgtgata gcagcgcagc gccgcttcca
     4321 cggcggaatt cttcccgccg acgatcagca gccgcttgcg gaagtaggtg tgcgggtcct
     4381 ggaaataatg gctgacgtgc ggcaggtctt cgcccggcac gttcagccgc cgcgggtgat
     4441 ccgtg
//
LOCUS       DATIMH010000993         4842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5151794, whole genome shotgun sequence.
ACCESSION   DATIMH010000993 DATIMH010000000
VERSION     DATIMH010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5151794"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="VLI90_17240"
     CDS             complement(<1..1735)
                     /locus_tag="VLI90_17240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV16010.1"
                     /translation="MSVVAVEPVTRAMRPSPADASPSHPAAPGARQRRVQRATNRVRR
                     DGKFFRLGAEKFYVKGVTYGPFEIDHVGQPLPNREQVEKDFRLMQDLGVNCVRIYHVP
                     PKWFLDLAMLCGLKVFLDVAWPKNISFDGDDDVIKAAHFAVRNAANQCGNHPAIFAIS
                     VVNEFPPDLVRFIGKEKVEDLIDELVCIVKDEAPDCLVTFANFPTTEYLRPREIDFVC
                     FNVYLHQDQVFRNYLARLQNIAGDKPLMLGEYGIDTHQEHTEDSQAEILSAHVKAVFE
                     EGLVGTFIFSFTDDWVTKGYRIDNWKFGLVRHDRTPKPSFEAVKSIFKKVPQTFDGAL
                     PSISVIICSYNGASTTESCLRSMERLRYPGELEVIFVDDGSTDNTQEILKMFPWVRNI
                     KQKNMGLSYARNVGMNAANGEIVVYTDSDCEADEDWLYYIAVAMKRSGHIGMGGPNLI
                     PDEGSWVADCVGLSPGGPTHVMIDDRTAEHVPGCNMAYYTFAAKQINGFDSQFRKAGD
                     DVDVIWRLQNLNYTIGFAPAAQVWHYRRNTIKAYLRQQRGYGEAEALLKYKHPDRFNT
                     LGASHWRGRIYG"
     gene            2135..2995
                     /locus_tag="VLI90_17245"
     CDS             2135..2995
                     /locus_tag="VLI90_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSV16011.1"
                     /translation="MPPTADEAIETFNTATEENQLSPLRDQQVVHLPAEGEVWMSGDI
                     HDHRRNFEKLVRVADLGNNPQRHLILHELIHGDHFDANGAEDSWNTLIKAAELKCDHP
                     GQVHFLLANHDLAQIHGEGIMKNGLSVCEAFTAGLKRDFGSVYARVNVAVTEFLLSLP
                     LAIRAPNGLFFCHSLPRDDQMDSFDFTIFDRPLTGPDYKRRTGPVYQLIWGRGVTPAG
                     VNIFLEKVNAQVVITGHQPQDTGFATNGDRHLIIASDHSQGVFLPLDLAATYTMDELV
                     ERLQKFVAIE"
     gene            complement(3094..4203)
                     /locus_tag="VLI90_17250"
     CDS             complement(3094..4203)
                     /locus_tag="VLI90_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16012.1"
                     /translation="MLHRRFILAAAYVCFLLIAKAPASAADDAAALYIKASKSTVVDC
                     PANSNLDYPGYPPFGPDWDRLAQAAWSQNAEVRRLVHEAASIDHANWPDPNGPSYLNG
                     LRAIANEVSDAALYQHLHGDDAGASQSLQDLLHLTKLIRQPSPPPTAVQHLVAAGIDA
                     LAAHSGMIMASDVTLTVDKTNAHILQVVGARRLIESLMDQPDVQDEIAEFKSEVTATA
                     PAKVRAARDRALEAFNRCNVERSFAAMSLACHIYRFEKQRWPQSLDELVPAYLPRIPL
                     DPWGDGKQTLGYTVVKGGLPNGWDRPLLFSRCESQDGLFYRTDTPAYDFTAYMIETPG
                     QPKPIRKRGGQFRDITRWSPDPEDQVGPTTRPLEH"
     gene            complement(4214..>4842)
                     /locus_tag="VLI90_17255"
     CDS             complement(4214..>4842)
                     /locus_tag="VLI90_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16013.1"
                     /translation="PGLPPPAMTDPLWVCGSTGPGEEPIVLDAYHALLQSFPTLRLAI
                     IPRHPQRFNEVAALIVQRGYPLLRRSARTRPTGGRPVILGDTMGELRKFYSLATVVFV
                     GRTLVDLGPRQHGSDMIEPAALAKPIIVGPWTHNFAEPMEKFREANAIREVMKHTSEL
                     KQTIGDWLKNPQDAAEVGRAARQVVRENQGATARHAGIILQHLPSIRQ"
BASE COUNT          885 a         1486 c         1549 g          922 t
ORIGIN      
        1 cgccgtagat ccgcccgcgc cagtggctgg cgccgagcgt gttgaagcgg tcggggtgct
       61 tgtacttgag cagcgcttcg gcctccccgt agccgcgctg ctgccggagg taggccttga
      121 tggtgttgcg gcggtagtgc cagacctggg cggcgggggc gaagccgatg gtgtagttca
      181 ggttctgcag ccgccagatc acgtccacgt cgtcgcccgc ttttcgaaac tggctgtcga
      241 agccgttgat ctgcttggcc gcgaacgtgt agtacgccat gttgcagccc ggcacgtgct
      301 cggcggtgcg gtcgtcgatc atcacgtgcg tcggcccgcc cgggctcagg cccacgcagt
      361 cggccaccca ggatccctca tccggaatca agttcggccc gcccatgccg atgtgaccgc
      421 tgcgtttcat cgccaccgcg atgtagtaca gccagtcctc atccgcctcg cagtcgctgt
      481 cggtgtagac gacgatctca ccgttggccg cgttcatgcc gacgttgcgg gcgtagctca
      541 ggcccatgtt cttctgcttg atgttccgca cccacgggaa catcttcaga atctcctgcg
      601 tgttgtccgt gctgccgtcg tcaacgaaga tgacttccag ttcgcccgga taacgcagcc
      661 gctccatcga tcgcaggcac gactcggtcg tgctcgcgcc gttgtacgag cagatgatca
      721 cgctgatgct gggcagcgcc ccatcgaacg tctgcggcac cttcttgaag atcgacttca
      781 ccgcctcgaa cgacggcttg ggtgtgcggt cgtggcgcac cagcccgaac ttccagttgt
      841 cgatgcgata gcccttggtg acccagtcgt cggtgaagct gaagatgaac gtgccgacca
      901 agccctcttc gaagaccgct ttcacatgcg cgctgaggat ctccgcctgg ctgtcttccg
      961 tgtgttcctg atgcgtgtcg atgccgtatt cgccaagcat cagcggctta tcgccggcga
     1021 tattctgcag ccgcgccagg taattgcgga acacctggtc ctggtgcagg tatacgttga
     1081 agcagacgaa gtcgatctcg cgcgggcgca ggtattctgt ggtcgggaaa ttcgcgaacg
     1141 tcaccaggca atcgggcgct tcgtccttga cgatgcagac cagctcgtcg atcaggtctt
     1201 cgaccttttc cttgccgata aaccgcacca ggtccggcgg aaattcgttg acgacgctga
     1261 tggcaaagat ggccgggtga ttgccgcact ggttggcggc gttgcgcacg gcgaaatggg
     1321 cggccttgat cacatcgtca tcgccgtcga agctgatgtt cttgggccag gcgacgtcga
     1381 ggaaaacctt cagcccgcac agcatcgcca gatcgaggaa ccattttggc gggacgtgat
     1441 agatgcggac gcagttgacg ccaaggtcct gcatcagccg gaaatctttt tcgacctgct
     1501 cgcgattggg cagcggctgg ccgacgtggt cgatctcgaa cgggccgtag gtgacgcctt
     1561 tgacgtagaa tttttccgcg cccaggcgga agaattttcc gtcgcggcgg acgcgattgg
     1621 tcgcgcgctg cacgcgccgc tgccgggcgc ccggggcggc cgggtgcgag ggcgaagcat
     1681 ccgcgggtga cgggcgcatc gcgcgcgtca caggttcaac agcaacaacc gacatcggga
     1741 cttcctgcat ccttccggtg ctatgcacta tcgacaaatt caaagccggc ggtgggaccg
     1801 agccataata tcttagccga tcttcgccgc aggcagaaaa accggcgaag aatcagccat
     1861 ctgcttagac agccggaatt tgcgggcgtt agcaacggtc gcgccggcgg cttcaacagc
     1921 tacgtccgat aaaccagctg cgttcggtgc caattggcag acggctaggg atgaaagatg
     1981 atgttgaacg cagagacgca gaggccgcag agatcagatc aaagactctg ttcctacctc
     2041 tgcggccttt gcgtctctgc gttcatattc actttgtttg aacagccgcg cccgcattga
     2101 cgaaaacgcc gccgggcaat aggaatgatg gcagatgccc cccaccgccg acgaagccat
     2161 tgagaccttc aacaccgcca cggaggagaa ccagcttagc ccgctgcgcg accagcaggt
     2221 cgtccacctg ccggccgagg gcgaagtgtg gatgagcggc gacatccacg accatcgccg
     2281 caacttcgaa aagctcgtcc gcgtcgccga cctgggcaac aacccccagc ggcatctgat
     2341 cctgcacgag ctgatccacg gcgaccactt cgacgccaat ggcgccgaag acagctggaa
     2401 cacgctcatc aaagccgcgg aactgaagtg cgaccacccc ggccaggtcc acttcctgct
     2461 ggccaaccac gacctcgccc agatccacgg cgaaggaatc atgaagaacg gcctgagcgt
     2521 ctgcgaagcg ttcaccgccg ggttgaagcg cgacttcggc agcgtctacg cgcgcgtgaa
     2581 cgtcgccgtc accgagttcc tgctgtcatt accgctggcg attcgcgcgc ccaatggcct
     2641 gttcttctgc cacagcctgc cgcgcgacga ccagatggac agcttcgact tcacgatctt
     2701 cgaccggccg ctgacaggac cggattacaa gcgccgcacc ggccccgtct atcaactgat
     2761 ctggggccgc ggcgtcacgc cggcgggcgt caacatattc ctggagaagg tgaacgcgca
     2821 ggtggtgatc accggccatc aaccgcagga caccggcttc gccaccaacg gcgatcggca
     2881 cctgatcatc gcatccgacc acagtcaagg cgtgttcctc cccctggacc tggcggcgac
     2941 gtacacgatg gacgagctgg tggagcggct gcaaaagttc gtcgcaattg aatgacgcta
     3001 ctcgaacaaa aaacgccgcg cgttcagccg ccgccgcctc gcggcgcgaa ttgcaataac
     3061 gtgatgctgg cgcatcccgc gatagggaac ggatcagtgt tccagcggcc gcgtggttgg
     3121 tccgacctgg tcttccggat cgggcgacca gcgggtgatg tcgcggaact gtccgccgcg
     3181 tttgcggatc ggcttcggtt ggccaggcgt ctcgatcatg tacgcggtga aatcatacgc
     3241 gggcgtgtcg gtgcggtaga acaatccatc ctgcgattcg cagcgtgaga acagcagcgg
     3301 gcgatcccag ccgtttggca ggccgccttt gaccacggtg tagccgagcg tctgcttgcc
     3361 gtcgccccag ggatcgagcg ggatgcgcgg caggtatgcc ggcaccagtt cgtccagcga
     3421 ttgcggccaa cgctgcttct cgaagcgata gatgtgacac gccagcgaca tcgcggcgaa
     3481 gctgcgttcg acgttgcagc ggttgaaggc ctcgagggcg cgatcgcgcg cggctcgcac
     3541 tttcgcgggc gcggtggcgg tgacctcaga tttgaattcc gcaatctcgt cctgcacgtc
     3601 cggctggtcc atcagcgatt cgatgagccg tcgcgctccg acgacctgca gaatatgagc
     3661 gtttgttttg tcgaccgtga gtgtcacgtc cgacgccata atcatgccgc tatgagcagc
     3721 cagcgcatca atgccagcgg caaccagatg ttgtacggcg gtgggtggag gtgacggctg
     3781 gcgaatcagc ttcgtcaggt gcagcaggtc ttgcagggat tggctcgccc cggcgtcgtc
     3841 gccgtgcagg tgctgataaa gcgcggcgtc gctcacttcg ttggcgatgg cgcgcaggcc
     3901 attcagatag gatggcccgt ttgggtccgg ccagttggca tgatcgatcg acgcggcttc
     3961 gtgaaccagc cggcgcacct cggcgttttg gctccatgcc gcctgcgcca gccggtccca
     4021 gtccgggcca aacggtgggt agcccggata gtccaaattc gaattggccg gacaatcaac
     4081 gacggtcgat ttcgacgctt tgatgtagag ggctgcagcg tcatcggctg ccgatgccgg
     4141 cgccttggcg atcaagagaa aacaaacgta ggcggcggcg aggatgaacc ttcgatgaag
     4201 catgggatcg cgctcattgc cggatcgacg gcagatgttg aaggatgata ccggcgtggc
     4261 gagcggtggc gccctgattt tcccgcacaa cctgccgggc ggctcgtccg acttcggcag
     4321 catcctgcgg gttcttcagc caatcgccga tcgtttgctt tagctcgctc gtatgcttca
     4381 tcacttcacg gatcgcgttc gcctcgcgga acttttccat cggctcggca aaattgtgcg
     4441 tccatgggcc gacgatgatg ggcttggcca gggcggcggg ttcgatcatg tcgctgccgt
     4501 gttggcgcgg gccgaggtcg accagcgtac ggccgacgaa gacgacggtg gcgaggctgt
     4561 agaatttgcg aagctcgccc atcgtgtcgc cgaggatgac gggccggccg ccggttggcc
     4621 gggtgcgcgc ggatcggcgc agcagaggat agccgcgctg cacgataagc gcggcgactt
     4681 cgttgaagcg ctgcggatgg cgcgggatga tggcgaggcg cagcgttggg aatgattgca
     4741 ggagcgcgtg ataggcgtcg aggacgatcg gctcttcgcc tgggccggtg gaaccgcaga
     4801 cccataaggg atccgtcata gccgggggcg gaagccccgg tc
//
LOCUS       DATIMH010000994         6732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_527440, whole genome shotgun sequence.
ACCESSION   DATIMH010000994 DATIMH010000000
VERSION     DATIMH010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527440"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..542)
                     /locus_tag="VLI90_17260"
     CDS             complement(30..542)
                     /locus_tag="VLI90_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16014.1"
                     /translation="MIGRTNSIIVGMLLPFALLGCQPAKPPPPREDPLAGLGGGLDTS
                     VQTMSPETAVRSAPCAARLQNIGGTLLLYYATHQQMPTRLDDLKPLADMDEPLELSCP
                     VSKKAYLYSPTGLAAVGKDKRIVVYDPEPSHEGRRWCLFMIPAKPGQALAVEVLEVPE
                     GLFRTFLPTG"
     gene            579..902
                     /locus_tag="VLI90_17265"
     CDS             579..902
                     /locus_tag="VLI90_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16015.1"
                     /translation="MLRAAYFLGQFPTANYYSLQKPSHGQIPLFFDARWRDARPHKNT
                     EGYWPIDKTTTDGMPEIAIKRHGRVANVSFLDGSTRTIPLPELWNLQWEPARTPPTML
                     PKVPW"
     gene            complement(1123..1416)
                     /locus_tag="VLI90_17270"
     CDS             complement(1123..1416)
                     /locus_tag="VLI90_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16016.1"
                     /translation="MRLGGWFNGDFNYDGVVDGGGYAQIDNAFLLQGSGLAQPAAAAA
                     GVLSVAAAKAPVARGVAAASDTRWSAVSFGDATIALRRDALVDEVMQLMVSRV"
     gene            complement(1722..3494)
                     /locus_tag="VLI90_17275"
     CDS             complement(1722..3494)
                     /locus_tag="VLI90_17275"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV16017.1"
                     /translation="MAEPLASALPAGSASPPGLSPATIKHAYGIDSIKFGSTTGDGSG
                     QTIAIITAYDNPRLLSSSDPNFSTSDLRKFDAQFGLPDPPSFRKVDQHGGTSYPGPSP
                     TWANEAALDVEWAHAVAPKANLLLVEAPSATTWTDLIAGPVSWARQQPGVSVISLSFA
                     GGEFSGETSYDSYLTTPSGHQGITFVAAAGDNGAAAMYPTVSPNVIGVGGTRITLSGS
                     SYVSESVWGSSGGGISTYESKPSYQSGVTQSSSKRTVPDVAFDADPNFSGVSAYDSYN
                     GGGSTPWYKMGGTSLGAPVWAGLIAITNQGRALQGKGSLEGRSQTLPTLYSISGNDFH
                     DITGGGNGGFAAGPGYDLITGRGTPKANTLVPDLGGLAQSPPSPASIAGNLFNDSNGN
                     AKRDSGEPNLANRTFYIDANKNGKFDSGEKTAKADSSGNYKFSSLAAGTYVIREQIPS
                     GWRVSLPTTTWSQTIAVSAGQNRSGVNFADTQKVLISGNVFNDSNGNKTKGSGESALS
                     GWRVYIDSNNDGKYESNEASTLTDSSGNYKFTTLSAGSFHLRVTPPSGVSGYSATTPS
                     GGVYTFTLAGGGLAMNKNFGERKA"
     gene            complement(3788..4012)
                     /locus_tag="VLI90_17280"
     CDS             complement(3788..4012)
                     /locus_tag="VLI90_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16018.1"
                     /translation="MTMNFKRLGKWITAGLGLAMLSGCVVYERPRYREVVYDDCPPPG
                     HVVVVEPVPTIVFGGGYHHGYYGGGHRHWR"
     gene            4163..4945
                     /locus_tag="VLI90_17285"
     CDS             4163..4945
                     /locus_tag="VLI90_17285"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HSV16019.1"
                     /translation="MRQHLFIAATGLLALVAATTRTTTNPSIRPASRGGWWMQRHQAI
                     CDRLKQGPVDLIFVGDSIIQNYDKHNPPDEDFQPTWNYFYGDRNAINMGFSGDHTGNV
                     LWRLQHGEVDGITPKVAVLLIGTNNTAGAKQTAQQTIAGIQADVALLHEKLPSAKVLL
                     LGILPSDITPEKGAADAAVNAALAQKYADSDYVRFLDIGPVFMRNGKLDASLFYDPRL
                     PPGKDGKPRGPLHPDTHGQRMMAEAIEPTLAKLMGDRSKVQP"
     gene            complement(4958..5122)
                     /locus_tag="VLI90_17290"
     CDS             complement(4958..5122)
                     /locus_tag="VLI90_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16020.1"
                     /translation="MWAKRFYLLSSLFFLGGAVLAIRSAISPINLLYVLGSAFFVIAA
                     SASVFGRGRE"
     gene            5173..5538
                     /locus_tag="VLI90_17295"
     CDS             5173..5538
                     /locus_tag="VLI90_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16021.1"
                     /translation="MLTRFAVAMMTVAAGIGGCATQATPAAVAPVAIAPAPAAKPAAP
                     VIVRLVSRHYTIVVSAGRTAPLYQVTDNSGKLLADGVTLAQLRLVDNNLYQELVPALA
                     PKAEADASMEGPAIGLAEK"
     gene            6183..>6732
                     /locus_tag="VLI90_17300"
     CDS             6183..>6732
                     /locus_tag="VLI90_17300"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HSV16022.1"
                     /translation="MQAFLKRIRRPKVLSLAGLLVAVLVAQLLFPSQAFASEAGLRLP
                     DLNQPKFFGIGGHTLLVYGLVICGLGLIFGMVIYQQLKNAPVHASMREVSELIYETCK
                     TYLRTQLKFILLLELFIGSVLILYFGFLAQDPISHQAGLGPMKVAIIVLFSLVGIFGS
                     YSVAWFGIRINTFANSRAAMGSL"
BASE COUNT         1256 a         2181 c         2052 g         1243 t
ORIGIN      
        1 tcgctgaagc gaccacgccg cgcatgcttt caacccgtcg gtaaaaacgt tcggaacaat
       61 ccctccggca cctccagcac ttccaccgcc agggcctgcc ccggcttcgc tgggatcatg
      121 aaaaggcacc accgccgccc ctcgtgcgat ggctcggggt cgtacacgac gatccgctta
      181 tctttcccca ccgccgccag tccggtgggg gaatacaggt acgctttctt cgacaccggg
      241 cagctcagct ccagcggttc atccatatcc gccagcggct tgagatcgtc cagccgcgtc
      301 ggcatctgct gatgcgtcgc gtagtacagc agcagcgtcc cgccgatgtt ttgcagccgg
      361 gccgcgcacg gggccgatcg caccgccgtc tccggcgaca tcgtctgcac cgacgtgtcc
      421 agcccaccgc cgagccccgc caatggatct tcccgcggcg gcggtggttt ggcgggttgg
      481 cagccgagga gcgcgaacgg caggagcatg cccacgatga ttgaattcgt tcgaccgatc
      541 acggctcaat cgtgacacga gcctcggggc catgcaagat gcttcgtgcg gcctacttcc
      601 tcggccagtt cccgacggcc aactactaca gcctgcagaa gccgtcgcac gggcagatcc
      661 cgctgttctt cgacgcccgc tggcgcgacg cgcgtccgca caagaacacc gaaggatatt
      721 ggccgatcga caagaccacg accgacggca tgcccgagat cgcgatcaag cggcatggca
      781 gagtcgcgaa cgtctcgttc ctggacggca gcacccgcac gatcccgctg ccggaactgt
      841 ggaacctgca atgggaaccc gcgcggaccc cgccgacgat gctgccgaag gtgccgtggt
      901 aatggaaagc gaggcgcgcc gcatgaacgt acgtgcgacg cgcgtgaagc aacgatccct
      961 ctccctcccg ctcgcacgtc cgtccaaggt ggacgtgcga gttgtttttt gcccaccggg
     1021 ctaaccgctt cagcggttag cgtgcccttc tcaatcatcg caaaaaagaa gaagccccgg
     1081 atctacatcc ggggctccgt catcaagaca cgtacgaaat aattaaacgc gtgagaccat
     1141 cagctgcatc acttcatcca ccagcgcatc tctgcgcagc gcgatggtcg cgtcgccaaa
     1201 gctcaccgca ctccaccgcg tatccgacgc cgccgcaacg cctcgcgcga ctggcgcctt
     1261 cgctgccgcc acactcagca cccccgccgc ggcggccgcc ggttgcgcca acccgctccc
     1321 ttgcagcagg aacgcgttgt cgatctgcgc gtaaccgccg ccgtcgacaa cgccgtcgta
     1381 gttgaagtcc ccgttgaacc acccgccgag gcgcatgttg aagccgcgga gggtgatttg
     1441 aaataccagc ggttagccac agcggttcgg aggtactcca acgacccaga tggcattgaa
     1501 tgcagagtga tgaatgaaga acggacgagg cgcaccaagc gcgtcgggga caaaaagccc
     1561 gcgcggcgcg gttacggagg ccgctccgac tggcgagcgg cggcttctct tgccagaatc
     1621 cgacgctcca ccgatccgtg cttttttaat gcgctggaag ccgctagaat tgtgccgttt
     1681 tgcaaagccc attcgatctc attctgtctc tttcgagcag attaggcttt gcgctcgccg
     1741 aaattcttgt tcatcgccag cccaccgccg gcgagggtga aggtgtagac gccgccgctg
     1801 ggggtggtcg cgctgtagcc actgacgccg ctgggcggcg tcacgcgcag gtggaacgag
     1861 ccggcgctaa gcgtggtgaa cttgtagttc ccgctggagt ccgtcagcgt gctggcttcg
     1921 ttgctctcgt acttgccgtc gttgttcgag tcgatgtaga cgcgccagcc gctcagcgcg
     1981 gattcgccgc tgcccttggt cttgttgccg tttgagtcat tgaagacatt gccgctgatc
     2041 aggacttttt gcgtgtcggc gaaattcacg ccgctgcgat tttgaccggc gctgacggcg
     2101 atcgtctgcg accaggtcgt cgtcggcagc gacacgcgcc agccgctggg gatctgctca
     2161 cggatcacat acgtcccggc ggcgaggctg ctgaacttgt agttaccgct cgaatcggcc
     2221 ttggcggtct tctcaccgct gtcgaatttg ccgttcttgt tcgcgtcgat gtagaacgtg
     2281 cgattggcga ggttcggctc gccgctgtcg cgcttcgcgt tgccgttgga gtcgttgaac
     2341 agattgccgg cgatcgacgc gggcgacggt ggcgattgcg ccaacccgcc caggtccggc
     2401 accagcgtgt tcgccttcgg tgtcccgcgg ccggtgatca agtcataccc gggccccgcg
     2461 gcgaacccgc cgttcccgcc gccggtgata tcgtggaaat catttccgct gatcgagtac
     2521 agcgtcggca gcgtttggct gcggccttcg agcgagcctt tcccttgcag cgcgcggcct
     2581 tggttggtga tggcaattaa ccccgcccac acaggggcgc cgaggctggt gccacccatc
     2641 ttgtaccacg gcgttgatcc tccgccgtta tacgagtcgt acgcggaaac gccgctgaaa
     2701 ttcgggtcgg cgtcgaacgc cacgtccggc accgtgcgct tgctgctgct ctgcgtcacg
     2761 ccgctttgat acgacggttt gctttcgtac gtgctgatgc cgccaccact gctgccccac
     2821 acggattcgc tgacatagct gctgccgctg agcgtgatgc gggtcccacc gacgccgatg
     2881 acgttgggcg agaccgtcgg gtacatcgcg gccgccccgt tatcacccgc ggcggcgaca
     2941 aacgtgatgc cctggtgacc gctgggcgtg gtgagatagc tgtcgtagct ggtctcgccc
     3001 gagaactcac cgccggcgaa gctgagcgaa atgaccgaaa cgcccggctg ctgccgcgcc
     3061 cagctgacgg ggccggcaat caggtcggtc cacgtcgttg cgctgggcgc ttcaactaga
     3121 agcaggttcg cctttggcgc gacggcgtgc gcccactcga cgtcgagcgc ggcttcattc
     3181 gcccacgtcg gcgacggacc tgggtaagac gtgccgccat gttgatcgac cttgcgaaac
     3241 gacggcgggt ccggcaggcc gaactgcgcg tcgaacttgc gcagatcgct ggtgctgaag
     3301 ttcgggtcgc tggagctgag cagccggggg ttgtcgtacg cggtgatgat ggcgatcgtc
     3361 tgccctgagc cgtcaccggt ggtgctgccg aatttgatcg agtcgatgcc gtaggcgtgc
     3421 ttgatcgtcg ccggcgacaa tcccggcggc gatgcgctgc ccgcgggcaa cgccgacgcc
     3481 agcggctcag ccactggtgt gagcgaaaac agacagcgct gctcgaggat ttcaaacagc
     3541 caagagacac ttcgggaacg agagcgccga ctcatacgca cgactccagc gacgatgacg
     3601 attggggtac tgctgtgaaa ctgagaccac tcgcgtgcaa agctgcggag accgccctca
     3661 ccggcggggc ttccctccgt caataagcat acggcaaagc gggcgtttgg gtgctgcaaa
     3721 aaaagaaaag gacgcgccgc ggcggcgcgt ccttctcgat taaagttgaa ctcgatcggc
     3781 ccggtgatca acgccaatgg cggtgcccgc ccccgtagta gccgtgatga taaccgccac
     3841 caaacacaat ggttgggacc ggctcgacca ccaccacatg ccccggcggc gggcagtcat
     3901 cgtaaaccac ctcacgatac cgcggccgct cgtaaacgac gcagccactg agcattgcca
     3961 aaccaagccc tgcggtaatc cacttcccta aacgcttgaa gttcatagtc atagtacgct
     4021 ccattccagc gatggattag acacgcgtgg gaggcgcagg ttagtgatcg caagcctttg
     4081 tggtgtgaac agttgccacc ggcgtcagct gtgcgctgca gctctttcat ttcgcgcgac
     4141 gcccgctacg ctttccacgc acatgcgtca acatctcttc atcgctgcaa ccggcctcct
     4201 cgcgctggtc gcggcgacca cccggaccac gaccaatcct tcaatccgcc ccgcgtcgcg
     4261 cggcggatgg tggatgcagc gccatcaagc gatctgcgac cgcctcaagc aaggcccggt
     4321 tgacctgatc ttcgtcggcg attcgatcat tcagaattac gacaagcaca acccgcccga
     4381 tgaggatttc cagccgacgt ggaattactt ttatggtgat cgcaacgcca tcaacatggg
     4441 cttcagcggc gaccacacgg gcaacgtgct gtggcgactc cagcacggcg aagtagatgg
     4501 catcacaccc aaagtcgcgg tgctgctgat cggcaccaac aacaccgccg gcgcgaagca
     4561 gacggcgcag cagacgatcg caggaatcca agcggacgtc gcgctgctgc acgagaaact
     4621 gccgagcgcg aaggtgctgc tgctgggcat cctgcccagt gacatcacgc cagaaaaggg
     4681 cgcagccgac gccgccgtga acgcggcgtt ggcgcaaaaa tacgcggact ccgactacgt
     4741 caggttcctc gacatcggcc cggtcttcat gcgcaacggc aagctggatg cgagcctttt
     4801 ctacgacccg cgcctacccc cgggcaaaga cggcaaaccg cgcggcccgc tgcacccgga
     4861 cacgcacggc cagcgcatga tggccgaggc catcgaaccg acgctggcga agctcatggg
     4921 cgaccgcagc aaggtgcagc cgtgagcggt tcgacgatca ttcgcgccca cggccaaaga
     4981 cgctcgcgct ggcggcgatc acgaagaacg cactgccaag cacgtacagc aggttgatcg
     5041 gcgagatcgc ggagcggatg gcaaggacag cgccgccgag gaagaacagg ctgctgagga
     5101 gataaaagcg cttagcccac acggtcgcgc ggtatggtag cggaaagatt cgtcgcgcac
     5161 gaaaggatgc agatgctcac gcgattcgcc gtcgcgatga tgacagtcgc cgccggaatc
     5221 ggcggatgcg cgacgcaggc gacgcctgcc gcggtagcgc cggttgcgat cgcgcccgcg
     5281 ccggcggcga aacccgcggc acccgtcatc gttcgcctcg tcagccggca ttacacgatc
     5341 gtcgtctccg ccgggcggac cgcgccgctc tatcaggtca ccgataactc tggcaagctc
     5401 ctcgccgacg gcgtgacgct tgcgcagctg cgactggttg ataacaacct gtatcaggag
     5461 ctggtccccg cgctcgcgcc caaggcggaa gcggatgcat cgatggaagg gccagcaatt
     5521 ggcctcgccg aaaagtagca tgcgcggtct ctccacacac cgggttcgga gcgtactcgc
     5581 gacgacccgg attcttgggc gcgtcgagta agaagaatcc gggtcgtcgg agtacctccg
     5641 aacccggcgt gggcgccttc attcactcca agcgtattcc ccccattcac cgagatcttc
     5701 cgccccatga tgaagctcgc cacgtgtacc cgcgattacc gccttgcatt gagaagagaa
     5761 tgtcagtggc cccaccgcct tggtgacgcc ccttacccgc cgttcagttt gattttcgtc
     5821 gcgcacgtcg gtggggaagg cgacaagtcc gcctgcatcg tcaccccgtc cttcggttgt
     5881 gtccgtctgt atcgagttgt ccatctattt cagccgcggc atgcgtctcg gtgagatctg
     5941 tcgttcggat tggccaatat atggttgtca aaaccgggcg ggcaaatgat aatcccctca
     6001 cagacgattg gtgatgcgaa gctgcgacac cgaggcggga ggaacgatcg aggcacccga
     6061 tggttcgccg gcaggaggcc gggccccaag actaacccat tggatcgggc aattgcttcg
     6121 gccatccttc gtccccccac gttgtcgggc gcacgtcacc tcagaaaaga aaggcccgag
     6181 gtatgcaggc attccttaag cggatccggc gtccgaaggt gttgtcgctg gccggattgc
     6241 tcgttgccgt tttggttgcg caactccttt ttccctccca ggcattcgcc agtgaagccg
     6301 gcctcagatt gccggacctc aatcagccaa agttcttcgg catcggcggg cacacgctgc
     6361 tggtgtacgg gctggttatc tgcgggctgg gcctgatctt cgggatggtg atttaccagc
     6421 agctgaaaaa tgcccccgtg catgcatcga tgcgcgaggt gagcgagctg atttacgaga
     6481 cgtgtaagac ctatctgcgg acgcagctga agttcatcct gctgctcgag ctgttcatcg
     6541 gttcggtatt gatcctgtat ttcggattcc tggcgcagga tccgatcagc caccaggcgg
     6601 ggctggggcc gatgaaggtg gcgatcatcg tcctgttcag cctcgtcggc atcttcggca
     6661 gctacagcgt cgcgtggttc ggcatccgca tcaacacgtt cgcaaatagt cgcgccgcca
     6721 tgggttccct cg
//
LOCUS       DATIMH010000995         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5726475, whole genome shotgun sequence.
ACCESSION   DATIMH010000995 DATIMH010000000
VERSION     DATIMH010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5726475"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VLI90_17305"
     CDS             <1..397
                     /locus_tag="VLI90_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16023.1"
                     /translation="QIKSAGRTVVLSTHYIDEAHQLCDRIAIIDRGRVVAVGQPDELV
                     AASKTPARIVIRTSKPLDASRLREIVPSSVLREHAGEWHISSNEVTRTVTAIVSMVDG
                     NELLDLQIHRPTLEDVYLELTGTPFGPSE"
     gene            394..1731
                     /locus_tag="VLI90_17310"
     CDS             394..1731
                     /locus_tag="VLI90_17310"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV16024.1"
                     /translation="MTAGLWQHFRLTLRLNLRSPRALVYGYIVPIFFLLAFGSVFRSG
                     VPPLMREMGQLITISTLGGACFGMPTALVAERERGVWRRYRLLPVSLGGLVAGTMLAR
                     FLIIASAAVMQIALARGIYGTPFPAHPLQLLIAFTFVAFAFLGLGLVIAALADNVPAV
                     QALGQAIFLPMIMIGGVGVPLRSLPAWAQQVAGFFPGRYAVESLQPCFNGNGLHHATF
                     GLIALTIIGLAACVAGAKLFRWDAGSHVSRASWAWVAVAVCAGFAVGFTARSTGHLRP
                     VPNLAGPLAGEPYESITEAQINSITFTDLNADDDTVTPLAHSLDSLSDEDRQRLANIR
                     AKLDAWPPAHDGPDAQRVRNILSVAAIADYTQDPLEGQIARVVFDRLQADFDKELLTR
                     ILTWIILHPEQGAAITSAPELDLQGRVNEDVLRDRAMLYAKKLLGRLVGKIVD"
     gene            1858..2340
                     /locus_tag="VLI90_17315"
     CDS             1858..2340
                     /locus_tag="VLI90_17315"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSV16025.1"
                     /translation="MTPQSILQPNTPAPDFSLPDKPGGTVNLRDYRGRPVVLVFYPAD
                     WSPVCGDQVELYNEIADEFAKHNAQLLGISVDGAWCHQAFANSRKLEFPLLADFEPKG
                     TVARAYGVYREQDGTTERALFVIDRDGVIRWSYLSPIDQNPGADGILAALEELEPAHA
                     "
BASE COUNT          438 a          860 c          735 g          433 t
ORIGIN      
        1 ccagatcaaa tccgccggcc gcaccgtcgt attgtcaaca cattacatcg acgaagcaca
       61 tcaactttgc gatcggatcg cgatcatcga ccgcggtcgc gtcgtcgccg tgggccagcc
      121 ggacgagctg gtcgcagcgt cgaagacgcc agcgagaatc gtcattcgca cgagcaaacc
      181 gctcgatgcg tcgcgcctgc gcgagatcgt tccctcaagc gtgctgcgag aacatgccgg
      241 cgaatggcac atcagcagca acgaggtcac gcgcaccgtc accgcgatag tcagcatggt
      301 cgacgggaat gaactgctcg acctgcaaat ccaccgcccg acgctggaag atgtttacct
      361 ggagctgacc ggcaccccgt tcggaccctc ggaatgaccg caggcctctg gcaacatttt
      421 cggctcacgc tccgcctgaa cctgcgcagc ccccgcgcgc tcgtgtacgg atacatcgtc
      481 ccgatcttct tcctgctcgc cttcggttcc gtcttccgtt ccggtgtgcc gccgttgatg
      541 cgcgagatgg gacaactcat cacgatcagc acgctcggcg gggcgtgctt cgggatgccg
      601 accgcgctcg tcgccgagcg cgaacgaggc gtctggcggc gataccgcct gctgcccgtc
      661 tcgctcggcg ggctcgtcgc cggcaccatg ctggcgcgat tcctcatcat cgcctcggcg
      721 gcggtgatgc agattgccct cgcccgcggc atctatggca cgccgttccc agcgcacccg
      781 cttcaactgc tgatcgcgtt caccttcgtc gccttcgcgt tcctcggcct cggactcgtc
      841 atcgccgcgc tggccgacaa cgtgcccgcg gtgcaggcgc tggggcaggc gatcttcctg
      901 cccatgatca tgatcggcgg cgtcggtgtg ccgctgcgat cgctgcccgc gtgggcacag
      961 caggtcgccg gattttttcc gggacgatac gcggtcgagt cgcttcaacc ctgcttcaac
     1021 ggcaacgggc ttcatcacgc gacgttcggc ttgatcgcgc tgacgatcat cggcctcgcc
     1081 gcgtgcgtcg ccggcgcaaa actcttccgc tgggacgccg gcagtcatgt gtcgcgcgca
     1141 tcatgggcgt gggtagccgt ggcagtgtgc gcggggtttg ccgtcggttt caccgccaga
     1201 tccaccggac atctgcggcc cgtgccgaac ctcgccggcc cacttgcggg cgaaccgtat
     1261 gaatcgatca ccgaggcgca gatcaacagc atcacgttca ccgatttaaa cgccgacgac
     1321 gacacggtca cgccgctggc tcacagcctc gacagtctga gcgatgaaga ccgccaacgg
     1381 cttgccaaca tccgcgccaa gctcgacgcc tggccgcccg cgcacgatgg ccccgatgcg
     1441 cagcgggtgc ggaacatcct gtctgttgcg gccatcgcgg attacacgca ggacccgctg
     1501 gaaggccaga tcgcccgcgt cgtctttgat cgcctgcagg cggattttga taaggagctg
     1561 ctgacgcgga ttctcacgtg gatcatcctg cacccggagc aaggcgcggc tatcaccagc
     1621 gcgccagaac tagaccttca aggccgcgtg aacgaagacg tgctgcgcga tcgcgcgatg
     1681 ctatacgcga agaagctgct gggcaggttg gtgggaaaga tcgtggatta gagaacccgc
     1741 cgcttgcggc ttggcagacc acgccgcttt gattgcgaag ccgcaaccgg ctaaacccgt
     1801 gcctacaatc gcacgcgccg cggcaccgtc caccaacacc aagggatctg gagagccatg
     1861 acacctcaat cgattcttca acccaacacc cccgccccgg atttttcgtt gcccgacaaa
     1921 cctggcggca ccgtcaacct ccgcgactac cgcggccggc cggtcgtgct cgtgttctac
     1981 cccgccgact ggagccccgt ctgcggcgat caagtcgagc tatacaacga gatcgccgac
     2041 gaattcgcga agcacaacgc ccagctgctc ggcatcagcg tcgatggcgc ctggtgccat
     2101 caggcgttcg cgaattcccg aaagctggag ttcccgctgc tggccgactt cgaacccaag
     2161 ggcaccgtcg cccgcgccta cggcgtctat cgcgaacaag acggcaccac cgaacgggcg
     2221 ctgttcgtga tcgatcgcga cggcgtcatc cgctggagct atctctcgcc gatcgatcaa
     2281 aaccccggcg ccgacggcat cctggcggca ctggaagaac tggagcctgc ccatgcttaa
     2341 gccaccaccc gcgccggcgc tgaagccgcc gttggattcc agtcgcgatc acatcctcgg
     2401 ctcggcgacg gcgccgatcg agctggtcga atatggcgac ttcgaatgtc cgcactgcgg
     2461 ccgggc
//
LOCUS       DATIMH010000996         4031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3704691, whole genome shotgun sequence.
ACCESSION   DATIMH010000996 DATIMH010000000
VERSION     DATIMH010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3704691"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VLI90_17320"
     CDS             <1..1013
                     /locus_tag="VLI90_17320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSV16026.1"
                     /translation="RYAGVVFSASQRILNDEARAQDVAQETFFRLMRQPKLVTHSLGG
                     WLHRSATHLAIDAKRSELARRQREKTYWLNKENEARSAEPKWEDVSPYVDQALIELPE
                     PNRTLLVRHFLQGTPQAELAAEMNTSAATISRKIKAGVEELQKLLKKKGIYVAMIALA
                     DFCMRETAKAAPAKMLAELGKMQMVGPVSVAPGAAAAAGNLPVLWPSTLKVPVKAAAD
                     STFKVVASCIGGAGVLVVFGVIVMAWSGHGPAIPSSAPPVTTESPAEATMDKPRLVVL
                     STSPADRVHAFERTSAIGKDVTVTFNDTTSQRLTWSGAHALILKQTGKSIEQLERESA
                     SR"
     gene            1175..1396
                     /gene="infA"
                     /locus_tag="VLI90_17325"
     CDS             1175..1396
                     /gene="infA"
                     /locus_tag="VLI90_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HSV16027.1"
                     /translation="MAKSEPIEVEAVVTLALPNAMFKLRLDNSDREILGIVSGKMRKH
                     FIRILPGDRVKVELSPYDLTKGRIIFRQK"
     gene            1574..2800
                     /gene="alr"
                     /locus_tag="VLI90_17330"
     CDS             1574..2800
                     /gene="alr"
                     /locus_tag="VLI90_17330"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSV16028.1"
                     /translation="MDARDALGEPAAHISRSALLHNAGVIRRAVGQDVKICAIVKANA
                     YGHDAQLVIDTLANFSADGASDSPPVDAFAVADLDEAAELPPVPQPLLVLRPVENVFL
                     GRQRAKIELAIRQGWLLTVANAAAADDIARIAMAVGKRAHLHVMVDTGMNRAGVGLDQ
                     LDSLMHKIDSRPSLRLAGIYTHFVTAEDAEHPLTIEQLAMFLDKTDGIAGQSAGRVIR
                     HAANSGAVFFSSSSHLDMVRPGISLYGVDPTGTPSMDRPLRPALRWTAPLIGIRDVPR
                     GGSVGYNQTWTADRDTRVGLVPVGYADGYQRAFSNRGVMLIHGSSARVIGRVSMDLTT
                     IDLSQVPMACIGDEVTVLDNDPLSGASVYQLAKWADTIPYEIFCRIGPRVRRVAVEPT
                     DGREVSATADDQGKIE"
     gene            complement(2850..3299)
                     /locus_tag="VLI90_17335"
     CDS             complement(2850..3299)
                     /locus_tag="VLI90_17335"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV16029.1"
                     /translation="MRHSLITANITGALWQRLRGKACTPYDSNLRLVVKKTGMRCYPD
                     TTVCCGAIVPDEEDDSSETITNPTALFEVLSKSTEAYDRGFKAENYRQIDSLRVYVLV
                     AQDAPHAELYERQTDGSWLLREVRGLNAALSVGARCRAAAGGDLRPR"
     gene            complement(3284..3418)
                     /locus_tag="VLI90_17340"
     CDS             complement(3284..3418)
                     /locus_tag="VLI90_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16030.1"
                     /translation="MSLAQPVKKYTPQEYYRLERAASYKSDYYAGEIFDRSAERSGIA
                     "
     gene            complement(3504..>4031)
                     /locus_tag="VLI90_17345"
     CDS             complement(3504..>4031)
                     /locus_tag="VLI90_17345"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSV16031.1"
                     /translation="MCRHFREDFGLVTRMARFHNVYGPHGTWQGGREKAPAAICRKVI
                     DAKDKNKLEIEIWGDGHQTRSFQYIDDCTLGIDLITHCPELIATPINLGSSELVSIND
                     LVSIVEKIGEVKLKRNYDLSAPKGVAGRNSDNTMIQQILGWEPNTTLRAGLAKTYAWI
                     ESQYRDHKAGKRTIV"
BASE COUNT          741 a         1286 c         1239 g          765 t
ORIGIN      
        1 gccgctacgc cggcgtcgtc ttctcggcgt ctcagcgcat cttgaacgac gaggcccgcg
       61 cgcaggacgt tgcgcaggaa acgttctttc gactgatgcg ccagccgaag ttggtgacgc
      121 attcgctcgg cggctggtta caccgatcgg cgacgcattt ggccattgac gcaaagcgaa
      181 gtgagctggc ccgccggcag cgcgagaaga cgtattggct gaacaaggaa aacgaggcgc
      241 gcagcgccga acccaagtgg gaagatgttt cgccgtacgt cgatcaggcg ctgatcgagc
      301 ttcctgagcc caaccgcacg ctgctggtcc ggcacttttt gcagggcacc ccgcaggcgg
      361 aactggcggc ggagatgaat acatcggccg ccaccatctc gcgaaagatc aaagcgggcg
      421 tcgaggaact tcaaaagctg ctgaagaaga aaggcatcta cgttgcgatg atcgcgctgg
      481 cggatttctg catgcgcgag actgccaagg ccgcccccgc gaaaatgctg gcggagctgg
      541 ggaagatgca gatggtcggt cccgtgtcgg tggcgcccgg cgccgcggcg gcggcgggaa
      601 atctgccggt gctgtggcca tcgacgctga aagtccccgt caaggcggcg gcagattcga
      661 cgttcaaggt cgtcgccagc tgcatcggcg gagccggtgt gctggtggtc ttcggcgtca
      721 tcgtcatggc gtggagcggg catggcccgg cgatcccgtc gagcgctcct ccggtaacga
      781 ccgaatcacc cgcggaagcc acgatggaca agccaaggct agtggtcctg tccacgtcgc
      841 cagccgatcg cgtgcacgcc ttcgagcgca cgagcgcgat cggcaaggac gtcaccgtca
      901 cgttcaacga caccacttct cagcggctga cctggtcggg cgcccacgcg ctgatcctca
      961 agcagaccgg caagagcatt gaacagctgg agcgggaatc ggcgtcgcgt tagtcgggac
     1021 gcccgagcaa gcctgaatcg cccgatccca tcccacctcc accgcacgcg gcaaaatagc
     1081 cggactttcc tgcacttcgc ttgtcgcgtg gccggtggtg ccgtaagatg tttgttcttc
     1141 atctgtctga ttcgaagtcc cgaaagaggt gtttgtggcc aagtccgagc cgattgaagt
     1201 ggaagccgtt gtcactttgg cgttgcccaa cgcgatgttc aagctgcgcc tcgacaactc
     1261 cgaccgcgag atcctcggca tcgtctcggg caaaatgcgc aagcatttca tccgtatcct
     1321 gccgggcgac cgcgtgaagg tcgagctgag cccgtacgac ctgaccaagg ggcggatcat
     1381 cttccggcag aagtaaacgc cctccccgcg acatcatcaa taacgaattt gctacgcatg
     1441 cgccgctacg gaacaaaact tcgcgggcgc ccaccacacc tttcacttcg accgcccctc
     1501 gatataatcg cgccgacacg gcccatggat gggtcgcgct gctgccgttc atctgccgga
     1561 atcgagcgtt cgcatggatg cgagagacgc gctgggggaa cccgctgcac acatttcgcg
     1621 gtcggctttg ctgcacaacg ccggcgtcat ccgccgggcc gttgggcagg atgtaaaaat
     1681 ctgcgccatc gtcaaagcca acgcgtatgg gcatgatgcc cagctggtca tcgatacgct
     1741 ggcgaacttt tcagccgatg gcgcgtcgga ttcaccgccg gttgacgcgt ttgccgtggc
     1801 cgatctggat gaggccgccg aactgccacc cgtgccgcag ccgctgctgg tcttgcggcc
     1861 ggtggagaat gtcttcctcg gccggcagcg cgcgaaaatc gaactggcca ttcgacaggg
     1921 atggttgctg accgtcgcca atgccgccgc cgctgacgac atcgcgcgca tcgcgatggc
     1981 cgttggcaag cgcgcacacc tgcacgtcat ggttgacacc ggcatgaatc gcgccggcgt
     2041 tggtttagat caactcgact cgctgatgca caagatcgac agccggccgt cgcttcggct
     2101 cgccgggatc tacacgcatt tcgtaaccgc cgaagacgcc gagcatccgc tgacgatcga
     2161 gcagctggcg atgttcctgg acaagacgga cggcattgcc ggtcaatccg ccgggcgcgt
     2221 catccgccac gccgccaact ccggcgccgt cttcttctcc tcttcatcgc acctggacat
     2281 ggttcgcccg ggcatcagcc tgtacggcgt cgatcccacc ggcacgccgt cgatggaccg
     2341 cccgctgcgc ccggcactgc gctggaccgc cccgctgatc ggcatccgcg atgtcccccg
     2401 cggcggcagc gtggggtaca accagacctg gaccgcagac cgcgacaccc gcgtcgggct
     2461 cgtcccggtc ggctacgccg atggttacca gcgcgccttc tccaaccgcg gcgtgatgct
     2521 gatccacggc agttctgccc gcgtgatcgg ccgcgtcagc atggacctga cgacgatcga
     2581 cctgagccag gtgccgatgg cctgcatcgg cgatgaagtg acggtcttgg acaacgaccc
     2641 gctcagcggc gccagcgttt atcaactcgc gaaatgggcc gacacgatac cctacgaaat
     2701 cttctgccgc atcggcccgc gcgtgcggcg ggtggcggtg gagccgacag acggacgcga
     2761 agtgtccgcc acggcggatg accaagggaa gatcgaataa tcagtggcgc ggcgtccgct
     2821 gcggcgcatt cgcctcggca gcggtgaagt caacgcggtc gaagatctcc gccagcggca
     2881 gctcgacacc gagctccgac cgacaaagcg gcgttcaagc cacgcacctc gcgcagcagc
     2941 caggaaccat ccgtctgtcg ctcgtacaat tccgcgtgcg gcgcatcctg cgccacaagc
     3001 acgtaaactc gaagcgaatc aatttggcga taattctccg ccttgaatcc gcggtcgtag
     3061 gcttcagtgg attttgacag aacctcgaat agcgcggtgg gattagtgat agtctcgctg
     3121 ctatcgtctt cctcatccgg cacaatcgca ccacagcaga cggtggtatc gggatagcag
     3181 cgcattccag tcttcttcac gaccagccga aggtttgagt cgtacggcgt gcaggctttg
     3241 ccgcggaggc gctgccaaag tgcgccggtg atatttgccg tgatcaggct atgcctgatc
     3301 gttccgccga cctatcgaag atctcgccgg cgtagtagtc gctcttgtag ctggcagcac
     3361 gttccagccg gtaatactcc tgcggcgtgt atttcttgac cggctgggca aggctcatcc
     3421 cagcatttta ccgagcgtga tgaacaagaa gaagccccgc atgcagatgc ggggctccgt
     3481 gttcgttcga gatttgcgat gaattagacg atcgtccgct tccccgcctt gtggtcgcgg
     3541 tattggcttt cgatccacgc atatgtcttg gccaggcccg cgcgcagcgt cgtattcggc
     3601 tcccagccga gaatctgctg gatcatcgta ttatcgctgt ttcgaccggc cacgcccttg
     3661 ggcgcgctca ggtcgtaatt ccgcttcagc ttcacttcgc caatcttctc gacgatgctg
     3721 accaggtcgt tgattgacac caactcgctc gatccgaggt tgatcggtgt cgcgatcagc
     3781 tccgggcagt gcgtgatcag gtcgatgccc agcgtgcagt cgtcgatgta ctggaagctg
     3841 cgcgtctgat gcccgtcgcc ccagatttcg atctccagct tgttcttgtc cttggcgtcg
     3901 atgacctttc ggcagatggc cgccggggct ttttcgcggc cgccctgcca cgtgccgtgc
     3961 gggccataga cgttgtggaa gcgggccatg cgcgtgacca ggccgaagtc ttcgcggaaa
     4021 tggcggcaca t
//
LOCUS       DATIMH010000997         6084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_236859, whole genome shotgun sequence.
ACCESSION   DATIMH010000997 DATIMH010000000
VERSION     DATIMH010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236859"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VLI90_17350"
     CDS             <1..1255
                     /locus_tag="VLI90_17350"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSV16032.1"
                     /translation="AMLAFKDDAPISPSKQNWPVNRGFEDHWGTIPGVENYYDPYGLV
                     HNEQTINIDRKDFYYTDFLTDHAVEMIDRYSRQPAPFFIYVAYTSPHWPMQAPEADVQ
                     KYASAYEKGWDAIRSERFARQVDMGLIKPQWTLSPRASNPALNDGASSVGLWENAPAR
                     SWQARRMAVYAAMIEHMDRGIGQLLDKLRQRNIEQNTLVIFLSDNGACQENVRPGWYD
                     IPEQTRDGEPIHVGNDPAVAPGPEGAYQSYGPAWANASNTPFRRFKHYTEEGGISAPF
                     IVRWPAANLQRGRIERDSVGDVIDLMPTLIEAAGAAYPKTMHGHDVLPEEGQSLLPLF
                     KGDAPASRTLFWEHEGNRAVRIGDWKLVGPHGEPWRLFNMIEDRTEGHDLSAEHPKKV
                     AELRAAYDAWAARAGVEPWPIKTSK"
     gene            complement(1263..1760)
                     /locus_tag="VLI90_17355"
     CDS             complement(1263..1760)
                     /locus_tag="VLI90_17355"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSV16033.1"
                     /translation="MPLDLLNGTSCFVDSNILYYTLVPTAGISQPCLALVDRAFAGEI
                     SFSVSIPVLSDAVHRVMVSEAAQLTGRDRSGIVGYLGRHPELIMRLIEYPQAIDRLSI
                     IPMRILPLEDHLLRNATALAVQHGLLTNDRMIVALMRQHQLTHLVTNDDDFDRVPGVI
                     VWKPR"
     gene            complement(1763..1990)
                     /locus_tag="VLI90_17360"
     CDS             complement(1763..1990)
                     /locus_tag="VLI90_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16034.1"
                     /translation="MVTLNAHFDGKTIVLDEPFTLALPSGTRLKVRVETVEEPAVVTP
                     ASRRFEPLNIRIDPQLSNAIALEPEFNIEES"
     gene            2107..3330
                     /locus_tag="VLI90_17365"
     CDS             2107..3330
                     /locus_tag="VLI90_17365"
                     /inference="COORDINATES: protein motif:HMM:NF042839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6786 family protein"
                     /protein_id="HSV16035.1"
                     /translation="MLLARSTIACALTAVLHVAAAHAQSNTTPNFSDDVMFLKTHGAV
                     VVLSDSAGQSKIAISPNLQARVMTSTAGGDDGASFGWINRQYFTDAAAGKGNPHISPW
                     GGEDRLWLGPEGGQFSIFFAKAASFDLEHWFTPKWIDVEPFETVSSTSTQATFQRKVS
                     FANYSGTPFDVRIDRTIRLLSADEVWNALHIPATPGVSVVAFESDNRLTNTGSANWTK
                     QSGLLSIWILGMFNATPMTTVVMPIRPGEGAAVNDDYFGKVPPDRLRVIDNVVYFKAD
                     AKDRSKIGIPPRRAKPIFGSYDGQHKVLTICQYTFHEAAQDYVNSAWKIQDNPFGGDV
                     ANSYNDGPPGDGKPQLGQFYEIETSSPAREMKRGETLSHTHRTMHFQGNERSLDSIAK
                     ATLGVSMAQIEAAMK"
     gene            3402..5675
                     /locus_tag="VLI90_17370"
     CDS             3402..5675
                     /locus_tag="VLI90_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16036.1"
                     /translation="MQSSTDHTTAFSRVHRPYTIKARRLAVFARLLLLAMICTTCAAR
                     ATVAADAPAPLVTGVVDRPQREERLSFQAQDPYRPRVHLDADVAMVYGIDKTLPARIK
                     TWRDQGYIVHVMTGVSWGEYQDYYFGRWDGKHREDEAQRMKNGEIIGHGGDVYYISPG
                     EDYGRYLCEGVKRALDAGAEAIHLEEPEYWVRAGWSGSFKRQWQKFYGEPWIDPDSSP
                     DAQYRASKLKYYLYRKTLAEVFDFVKTYGKAHNRDVKCYVPTHSMINYASWGIVSPES
                     SLLDVGADGFIAQVWTGTARTENFYNGIERQRTFETAFLEYGAMQNIVRASGKRVWYL
                     NDPIEDDPHHSWWDYRTNWESTLVASLLQPEVWHYEIMPWPHRIFETKYPATQPGTAG
                     RADANVPRIPIPEDYATELQAVISAMGDMRQPADRVGWEHVGTLGSGVLVSDTLMFQR
                     WGKTASDSHLGHFYGLALPLLMRGVPVEPVQIETAQLDRYKTLLLSYEGQKPPKPEFH
                     DTLAAWVKSGGALIVIDDDRDPFNSVREWWNTGDMHYATPRHHLFDKLGLSYDSTGEH
                     KVSQGTVIYQRRSPSRLSRSRDGAAIVVHDVQHAMAATGQTWKDSSALVLRRGPYVIA
                     AGMDAATDLPPVTLKGRFIPLFDAAQPIVHEFTVTPGTRGLLVDLDRYGKDDVGVIAA
                     ACHVTNQTVTGESITFDTIGQAETNAVVSVLLPRAPTGVTIDGTSQAAGAFDYNDGVL
                     RVRFPNRAQTLHVAVSR"
     gene            5611..>6084
                     /locus_tag="VLI90_17375"
     CDS             5611..>6084
                     /locus_tag="VLI90_17375"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HSV16037.1"
                     /translation="MASCACGSRTARRRSTSPFRDDGVLSRVEPQCEAASELIIVGSD
                     LPNRIECVTSQPVQITGGGGNDTIVAGGGPLTIDGGAGNDLIDASQNTSGATLSGGDG
                     NDTLLGGSAADNLFGGNGNDRLVGNAGNDTMDGGAGSDTMIGGAGTDVVDYTKRSA"
BASE COUNT         1259 a         1905 c         1898 g         1022 t
ORIGIN      
        1 ggcgatgctg gcgttcaagg acgatgcgcc gatctcaccg tcgaagcaga attggccggt
       61 caatcgcgga ttcgaagatc actggggcac catccccggc gtggaaaatt attacgaccc
      121 ttacggcctg gtgcacaacg agcagacgat caacatcgat cgcaaggatt tctactacac
      181 ggatttcctc accgatcatg cggtcgagat gatcgatcgc tattcgcgac agccggcgcc
      241 gttcttcatc tatgtcgcgt acacttcgcc gcattggccg atgcaggcgc cggaggcgga
      301 cgtgcaaaag tacgcgagcg cgtacgaaaa aggctgggat gcgattcgct ccgagcgttt
      361 cgcccggcag gtggacatgg gcctgatcaa gccgcagtgg acgctgtcgc cgcgggcgtc
      421 gaacccggcg ttgaacgatg gcgcgagttc cgtcggtctg tgggagaacg cgccggcgcg
      481 atcgtggcaa gcgcggcgga tggccgtgta cgcggcgatg atcgagcaca tggaccgcgg
      541 catcgggcag ctgctcgaca agctgcgcca gcggaacatc gagcagaaca cgctggtcat
      601 ttttctctcc gacaacggcg cctgccagga gaacgtccgc cccggctggt acgacattcc
      661 cgagcagacg cgcgacggtg agccaatcca cgtcggcaac gaccccgccg tcgcgcccgg
      721 acccgagggc gcctaccaaa gctacggccc cgcatgggcg aacgcgtcaa atacgccgtt
      781 ccgccggttc aagcattaca ccgaagaagg cggcatctcc gcgccgttca tcgtgcgctg
      841 gcccgcggcg aaccttcagc gcggccgcat cgaacgcgac agcgtcggcg atgtgatcga
      901 cctgatgccc acgctgatcg aagccgccgg ggcggcgtat cccaaaacaa tgcacggcca
      961 cgatgtgctg cccgaggaag gccaaagcct gctgccgctg ttcaaaggtg acgcgccggc
     1021 gagtcgcacg ctcttctggg aacacgaagg caatcgcgcc gtgcgcatcg gcgactggaa
     1081 actcgtcggc ccccacggcg aaccgtggcg gctgttcaac atgattgaag atcgcacgga
     1141 aggccacgac ctgtcggccg agcatcccaa gaaagtcgcg gagctgcgcg ccgcgtacga
     1201 tgcgtgggcc gcccgggcgg gcgtggagcc gtggccgatc aagacttcaa agtgatggtc
     1261 ttctagcgcg gcttccaaac gattacgccg ggaacgcgat cgaagtcatc atcgttggta
     1321 accagatgcg tgagttgatg ctgccgcatg agtgcaacga tcatcctgtc gttagtgagc
     1381 aggccgtgct gaacggcaag cgcggtggca ttgcgcagca gatgatcttc gagaggcagg
     1441 atcctcatcg gtatgatcga aaggcgatca atggcctgcg gatattcaat gagccgcatg
     1501 atcagttctg ggtgccttcc cagatatccg acgatgccgg agcgatcgcg gcccgtgagt
     1561 tgggccgcct ccgataccat cactctgtga acggcgtcgc tcagaacggg aatacttacc
     1621 gagaacgaaa tctcgccggc aaacgcgcga tcgaccaacg ccaagcacgg ctgggagatg
     1681 ccggcggttg gcaccagcgt gtaataaagg atgttcgaat caacaaagca gctcgtgccg
     1741 ttcagaagat cgagcggcat gttcaggatt cctcgatgtt gaattcaggt tccaacgcga
     1801 tcgcattcga caactgcgga tcgatgcgaa tgttcaacgg ttcgaagcgc cgtgatgctg
     1861 gagtgacaac cgccggctct tcaacggtct ccacccgaac cttcaggcgt gtgcccgaag
     1921 gcagcgcaag cgtgaagggc tcgtccagaa cgatggtttt gccgtcgaag tgggcattca
     1981 aggtcaccat gaacaatatt atatccgcgg cgtgatcgac aaaccatttg ccctagcggc
     2041 aacgtctggg atcaatgacg gcttaggaat gcggaactga tcaggagaac aatgatggaa
     2101 cgaatcatgt tgctggccag gtctacgatc gcgtgcgcat tgaccgcggt actgcatgtt
     2161 gcggctgcgc atgctcaatc taacactaca cccaacttca gcgacgatgt catgtttctc
     2221 aaaacccacg gcgcggtcgt cgtgttgagc gacagcgccg gccaatccaa gatcgcgatc
     2281 tcgccgaacc tgcaggcccg tgtgatgacc agcacggcgg gcggcgatga tggcgccagc
     2341 ttcgggtgga ttaaccgaca atacttcacc gacgccgccg cggggaaggg gaacccgcac
     2401 atcagcccgt ggggcggtga agatcgcctt tggctcgggc ccgagggggg gcaattttcg
     2461 atcttcttcg cgaaggccgc atcgtttgat ctcgagcact ggttcacgcc gaagtggatc
     2521 gacgtggagc ccttcgagac ggtgtcgagc acatcgacgc aggcgacgtt tcagcgaaag
     2581 gtgtctttcg cgaactacag cggcacgccg ttcgacgtgc ggatcgatcg cacaatccgg
     2641 ctactcagcg ccgatgaagt ttggaacgcg ctgcacatcc ccgccacccc gggcgtgagc
     2701 gtcgtagcat tcgaaagcga caatcgcctc accaacacgg gcagcgccaa ttggacaaag
     2761 caatccggcc tgctgtcaat ctggatcctc ggcatgttca acgccacgcc gatgacgacg
     2821 gtggtcatgc cgattcgccc cggcgaaggc gcggccgtca atgacgatta cttcggcaaa
     2881 gtgccgccgg atcggctgcg cgtgatcgac aacgtcgtct acttcaaagc cgacgcgaag
     2941 gatcgaagca agatcggcat ccccccgcgc cgggccaagc cgatcttcgg cagctacgac
     3001 ggtcagcaca aagtgctgac gatctgccaa tacacgttcc acgaggccgc gcaggactac
     3061 gtcaactccg cgtggaagat ccaggacaat cccttcggcg gcgacgtcgc caacagctac
     3121 aacgacggcc cgcccggcga cggcaaaccg cagctgggcc agttctacga aatagaaacc
     3181 agctcacccg cgcgagagat gaagcgagga gaaactctgt cgcacacgca ccgcacgatg
     3241 cattttcaag gcaatgaacg gtcgctcgat tcgatcgcca aagccacgct gggcgtgagc
     3301 atggcgcaga tcgaagcggc gatgaagtga cgcgcgctag cgcgcgctcc ggcaacaaag
     3361 cccacggccc tgccgtggga cccggcgtaa aaggagaggg catgcagagc agtacggatc
     3421 acacgaccgc cttttcccgg gtgcacaggc cgtatacgat caaagcccgg cggctggcgg
     3481 tgttcgcacg cttgctgctg cttgccatga tttgcaccac ttgcgcagca cgcgccaccg
     3541 ttgccgccga cgcgccagcg ccgctcgtca ccggcgtcgt cgatcgtccc caacgcgaag
     3601 agcgcctctc gtttcaagcc caggatcctt accgcccgcg cgtgcatctg gacgccgacg
     3661 tggcgatggt ctacggcatc gacaaaacgc tgccggcccg catcaaaacc tggcgcgacc
     3721 agggctacat cgtgcacgtg atgaccggcg tctcctgggg cgaatatcag gattattatt
     3781 tcggccgatg ggacggcaag catcgcgagg acgaagccca gcggatgaag aacggcgaga
     3841 tcatcggcca cggcggcgat gtttactaca tctcgcccgg cgaagattac gggcgatatc
     3901 tctgcgaggg cgtcaagcgc gcgcttgatg cgggcgccga agcgatccac ctcgaagagc
     3961 ctgagtactg ggtccgcgcg gggtggtcgg gcagcttcaa acgccagtgg cagaagtttt
     4021 atggcgaacc gtggatcgac cccgattcct cgcccgatgc gcagtatcgc gccagcaagc
     4081 tcaaatacta tctgtatcgc aagacgctgg cggaggtctt cgacttcgtc aaaacgtatg
     4141 gcaaggcgca caaccgcgac gtgaagtgct atgtgccgac gcactcgatg atcaactacg
     4201 ccagctgggg catcgtcagc ccggagtcgt ccctgctgga cgtcggcgcc gacggcttca
     4261 tcgcgcaggt ctggacgggc accgcgcgca ccgaaaactt ctacaacggc atcgagcgcc
     4321 agcgcacgtt cgagaccgcg ttcctcgagt acggcgcgat gcagaacatc gtgcgagcca
     4381 gcggcaaacg cgtctggtat ctcaatgatc cgatcgagga tgatccgcac cattcctggt
     4441 gggattaccg aacgaattgg gaaagcacgc tggtggcgtc gctgctgcaa ccggaggtgt
     4501 ggcactacga gatcatgcct tggccgcacc ggatttttga gacgaagtat ccggccacgc
     4561 agccgggcac ggccggtcgc gcggatgcca acgtgccgcg catcccgatc ccggaggatt
     4621 acgccaccga gcttcaagcc gtcatctccg cgatgggcga catgcgccag cccgccgatc
     4681 gcgtggggtg ggaacacgtc ggcacgctgg gcagcggtgt gctcgtgagc gacacgctga
     4741 tgtttcagcg ctgggggaag accgcatcgg actcgcacct cggtcatttt tacggtttgg
     4801 ccctgccgct gctgatgcgc ggcgtgccgg ttgaaccggt gcagatcgag accgcccaac
     4861 tcgaccgcta caagacgctg ctgctgagct atgaagggca gaaaccgcca aagcctgagt
     4921 tccacgacac gctggccgcg tgggtgaaat cgggcggcgc gctgatcgtc atcgatgacg
     4981 atcgcgatcc gttcaacagc gtgcgcgaat ggtggaacac cggcgacatg cactacgcca
     5041 cgccgcggca ccatttgttc gataagcttg gcctgtctta cgattcgacc ggtgagcaca
     5101 aggtcagcca gggcacagtg atctaccaac gccgctcgcc gtcgcggttg agtcgctcca
     5161 gagacggtgc ggcgatcgtc gttcacgatg tgcaacacgc gatggccgcc accggccaga
     5221 cgtggaagga ttcgtctgcc ctggtcctgc gccgcggacc gtacgtcatc gccgccggga
     5281 tggacgccgc gacggacctg ccgccggtca cgctcaaggg gcgtttcatc ccgctcttcg
     5341 acgccgccca gccgatcgtc cacgaattca ccgtgacccc cggcacgcgc gggttgctgg
     5401 tcgatctcga ccgctacggg aaagacgacg tcggcgtcat cgccgccgcc tgccatgtca
     5461 ccaaccaaac ggtgaccggc gaatcgatca ccttcgacac gatcggccag gctgaaacca
     5521 acgccgtcgt cagcgtgcta ctgccgcgcg cgcccaccgg cgtgacgatc gacggcacgt
     5581 cgcaagcggc cggcgctttc gattacaacg atggcgtcct gcgcgtgcgg ttcccgaacc
     5641 gcgcgcagac gctccacgtc gccgtttcgc gatgacgggg ttctatcgag ggtcgagccg
     5701 cagtgcgagg cggcatccga gctaataata gtcggcagcg accttccaaa tcgcatcgag
     5761 tgtgtcacga gccagccggt acagatcacc ggcggcggtg gcaatgacac tatcgtcgcc
     5821 ggcggtggtc cgctcacgat cgacggaggt gcgggcaatg acctgatcga cgcgagccag
     5881 aacacctccg gcgcgacgct ttcgggcgga gacggcaacg ataccctcct aggcggtagt
     5941 gccgccgaca acctcttcgg cggcaatggc aacgaccggc tcgtcggcaa cgccggcaat
     6001 gacacgatgg acggcggcgc cggcagcgac acgatgatcg gcggcgctgg caccgatgtc
     6061 gtcgattaca ccaagcgcag cgcc
//
LOCUS       DATIMH010000998         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3987559, whole genome shotgun sequence.
ACCESSION   DATIMH010000998 DATIMH010000000
VERSION     DATIMH010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3987559"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VLI90_17380"
     CDS             complement(<1..1035)
                     /locus_tag="VLI90_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16038.1"
                     /translation="MAEALHHHGELLDVPTRTRWRDRLASAVKFLDGWMTMETGNINY
                     PVTSSLAFTVAGEVLGDHHYLDRGREFAHHALDYFTANHLLFGENHPATFVTPKGCRP
                     IDLGYNVEESLPSLALYAMRTKDQPVLDAVVASLAAHMEFMLPDGSWDNSWGSRMFKW
                     TWWGSRTSDGCFPAYALLADREPRFAEVGRHNLALMQSCTHDGLLFGGPHLHLNGDPP
                     CVHHTFTHAKALATVLDAGGPVAVADRAALPRDSASGVKTFDEIQTRLIAVGPWRATL
                     TDYDLVYYKSPTGHPTGGTMSLLYHTALGPICVASMIEYALVEANNMQKHGDYPTMPL
                     TPRVEVIIDGQ"
     gene            1329..2039
                     /locus_tag="VLI90_17385"
     CDS             1329..2039
                     /locus_tag="VLI90_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16039.1"
                     /translation="MATLEQGATLGSVAEIIHAAHVLGPAAWGREPMPEAHMVGDIED
                     RLKAQLVDLFRMLHDRQVPYVLVGGIAMLTYIEGRNTKDVDLVLSVDSLAKLPELVIT
                     DRNREFARGKFGDLLVDLLFTDNPVFKLVQDRHTTTHRFLEMDVRSATVEGLILLKLF
                     ALPWLYQQGRWEKIFRYEADVAILYERSRPDLGPLFDELKAYLKKEQLQELRNIVGDI
                     QRRIERVDQAKKSGSEGK"
     gene            complement(2051..2758)
                     /locus_tag="VLI90_17390"
     CDS             complement(2051..2758)
                     /locus_tag="VLI90_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16040.1"
                     /translation="MTALIESQQRRRDEFETELARDKEELTREIENARAEWDAERKSR
                     EAEQKEFAAAEQKRREREREEYRYAFAREQHLAKDKFADEMAKAEKSLAERTADAERQ
                     LSLREQAVAEREQELDALRARVEASPKELDTAVARAVKEATDRARADFTAREELLKRE
                     FAGERNVLTTRITALEQTVKEQSEQITRFAGQAEKAYAQVQDIAVKAIEGSAASKSLA
                     GLQQLIADQSRRGSQEK"
     gene            2774..3268
                     /locus_tag="VLI90_17395"
     CDS             2774..3268
                     /locus_tag="VLI90_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16041.1"
                     /translation="MDIVDFLELRFLGEDRSLDLHVLSDFFFEAIGKLAERLADAGAQ
                     LTDRFDDAFRRQRIGGDDGFANGLGLDLVFGLGLGVAAVFDFLQRLRVGFQRLLTGRS
                     GGTLSGSRFVALFGGFLSHGGSLSQSRKFDKRGVRSEQTRGIKLACARGAARNRPTSW
                     PAVQ"
     gene            complement(3375..3581)
                     /locus_tag="VLI90_17400"
     CDS             complement(3375..3581)
                     /locus_tag="VLI90_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16042.1"
                     /translation="MTYRGHIKNGQVALDEPVSLPEGAEVSVVVIEKKVRITRPKDRQ
                     PLGKFRSIELQGGPLSDDIIRDRR"
     gene            complement(3782..>4016)
                     /locus_tag="VLI90_17405"
     rRNA            complement(3782..>4016)
                     /locus_tag="VLI90_17405"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          723 a         1149 c         1288 g          856 t
ORIGIN      
        1 ttgcccgtcg attattactt caacgcgagg cgtcagcggc atcgtgggat agtcgccgtg
       61 cttttgcatg ttgttcgctt cgaccagcgc gtattcaatc atgctggcga cgcagatggg
      121 gccgagggcg gtgtgataca gcagcgacat tgttccgccg gtgggatgac cggtgggaga
      181 cttgtagtag accaagtcgt agtcggtgag tgttgcccgc cacgggccga cggcgatcag
      241 ccgggtttgg atttcgtcga aggttttgac gccgctcgcg ctgtcgcgcg gcaacgcggc
      301 gcggtcggcc acggcgacgg gaccgccggc gtcgagcacg gtggcgagcg ccttggcgtg
      361 ggtgaaggtg tggtgcacgc acggcggatc gccgttgagg tgcaggtgcg gaccgccgaa
      421 gagcaggcca tcgtgcgtgc aggattgcat gagcgcgaga ttgtggcggc ctacttccgc
      481 gaagcgcggc tcgcggtcgg ccagcagcgc gtacgcggga aagcagccgt cgctggtgcg
      541 gctgccccac cacgtccact tgaacatccg gctgccccag ctgttgtccc acgacccgtc
      601 cggcagcatg aattccatgt gggccgcgag agatgcgacg acggcgtcca gcaccggctg
      661 gtctttcgtg cgcatcgcgt acagcgccag cgacggcagc gattcttcaa cgttgtaacc
      721 caggtcgatc gggcggcagc ccttgggtgt gacgaatgtc gcggggtggt tctcgccgaa
      781 gagcagatga ttggcggtga aataatcgag tgcgtgatgc gcgaactccc ggccgcggtc
      841 gagataatgg tgatcgccca gcacttcccc ggcgacggtg aacgcgagcg aggacgtgac
      901 ggggtaattg atgttgccgg tttccatcgt catccagccg tcgaggaatt tgacggcgga
      961 tgccagacga tcacgccaac gcgtgcgcgt cgggacatcg agcagttcgc catgatgatg
     1021 caacgcctcg gccacccggc cacacgccca cgcggctaca tggcaagcag gtcattccat
     1081 gatgaactcc ttttagttca ctggatcacg cgggtacact gacgcaccca agacgccaag
     1141 acgcaaagga aagacaattc gaatttctat cgcagacacg cgaagaggtg ccacaggttc
     1201 aagcatgcct ccgcgtttct ggccccggcg gttcaaccct tttctttctt cccttggcgt
     1261 cttggcgcaa acagagcacg cgcgcaccac aagcgcaagc taacgaagcg cccggtacaa
     1321 tagcggccat ggcaacgctg gaacaaggcg ccacgttggg cagcgtggcg gagatcatcc
     1381 acgcggccca tgtactgggc ccggcggcgt gggggcgcga accgatgccg gaggcgcaca
     1441 tggtgggcga tatcgaagac cggctcaagg cacagctggt cgacctgttc cgcatgcttc
     1501 acgatcggca ggtgccttac gtgctggtcg gcggcatcgc gatgctgacg tacatcgagg
     1561 ggcggaatac caaggatgtc gatctggtgc tgtcagtcga ttcgctcgcg aagctgccgg
     1621 agcttgtgat cacggatcgc aatcgggagt tcgcacgggg gaagttcggc gacctgcttg
     1681 tggacctgct gttcacggac aatcccgtgt tcaagctcgt gcaggacagg catacgacaa
     1741 cgcaccgttt tcttgaaatg gatgtccgga gcgcaacggt ggaaggcctg attctcctga
     1801 agttatttgc gcttccctgg ctgtatcagc aggggcggtg ggaaaagatt tttcgctatg
     1861 aagcggacgt tgcgatcttg tatgagcgga gtcgaccgga ccttgggcca ctgttcgatg
     1921 agctaaaggc atatctgaag aaggaacagt tgcaggagtt gcgcaatatc gtgggtgata
     1981 tccagcgccg gatcgagcgg gtggatcagg caaaaaagtc cggtagcgag ggaaagtaga
     2041 gtcgcgccct tcatttctcc tgcgagccgc ggcgggattg gtcggcgatg agttgttgca
     2101 ggccggcgag cgactttgag gcggcggagc cttcgatcgc tttcactgcg atgtcctgaa
     2161 cctgcgcgta cgccttctcc gcctggccgg cgaagcgggt gatctgctcg gactgctcct
     2221 tgaccgtttg ctcgagggcg gtgatgcgcg tcgtcagcac gttacgttcg ccggcgaatt
     2281 cgcgcttcag cagttcttca cgggcggtga agtcggcgcg ggcgcggtca gtggcttctt
     2341 tgaccgcgcg ggcgacggcg gtgtcgagtt cttttggcga tgcctccacg cgggcacgca
     2401 gcgcatccag ttcctgctcg cgctcggcaa ccgcctgctc gcgcagcgac agttggcgct
     2461 cggcgtcggc ggtgcgctcg gcgagggatt tttcggcctt ggccatctcg tcggcaaact
     2521 tgtccttggc cagatgctgt tcgcgggcga aggcgtagcg atattcctcg cgttcgcgtt
     2581 cacgccgctt ttgttcggcg gcggcgaatt ccttctgctc ggcttcgcgc gacttgcgct
     2641 cggcatccca ctcggcgcgg gcgttttcga tttcgcgggt cagctcttcc ttgtccctgg
     2701 ccaactcggt ttcgaattcg tcacggcggc gctgctgcga ttcgatcagc gccgtcaaag
     2761 tggaggcggc cttttggata tcgtagattt ccttgagctc cgcttccttg gcgaggatcg
     2821 ctcgctggat ctgcacgtac ttagcgactt cttcttcgag gcgatcggaa agttggccga
     2881 gcgtcttgcc gacgctggcg cgcagctcac cgatcgattt gacgacgcct tccgtcgaca
     2941 gcgcatcggc ggtgacgacg gcttcgcgaa cggtcttggc ttggatcttg tcttcgggct
     3001 tggcctcggc gtcgccgcgg tcttcgactt ccttcagcgt ttgcgcgtag gtttccagcg
     3061 tctgctgacg ggcagaagcg gcgggacgct ttcggggagc cggtttgtcg ctcttttcgg
     3121 tggatttctc agccatggcg ggagcctatc gcagtcgcgg aaattcgaca aacgcggcgt
     3181 gagaagtgaa cagacgcgcg ggatcaagtt ggcatgcgcc cgcggcgcag caaggaaccg
     3241 gccaacatct tggccagccg tgcaatgatc aggtgctcgg acccatcaat cagcgacgag
     3301 acccatcgtg acccgagctc gtttcgatac agcttcacca gtgctgagga atcgacaaag
     3361 aaggtgggca tggcttaccg gcggtcgcga ataatgtcat ccgacagcgg tccgccttgc
     3421 aattcgatgg atcggaattt gccaaggggc tggcgatctt tcgggcgcgt gatgcgcact
     3481 tttttctcaa tgaccacaac gcttacttcg gcaccttccg gcagcgacac cggttcatcc
     3541 aaagcgacct gcccgttttt gatgtgccct cggtaagtca tagaacaatt ataccgtccc
     3601 aatttggcgc agcaaaaaag cccggatctt tcgatccggg ctttttgctt gctctttgca
     3661 agtgaatagt cggaaaccat ccgccggcgt taccgtcggc gtttgagtct gttaggactc
     3721 acgagactgc gttgacattc ggtcagcggt ttgcaccgat gacgttctaa gaatccctta
     3781 gaaaggaggt gatccagccg caggttcccc tacggctacc ttgttacgac ttagtcccag
     3841 tcaccgaacc tagcgtaggt acctctgtat agaggcaact tcggctattt ccggctccca
     3901 tgacttgacg ggcggtgtgt acaaggctca ggaacacatt caccgcggcg tagctgatcc
     3961 gcgattacta gcgattccaa cttcacggag gcgagttgca gcctccgatc tgaact
//
LOCUS       DATIMH010000999         5729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5153053, whole genome shotgun sequence.
ACCESSION   DATIMH010000999 DATIMH010000000
VERSION     DATIMH010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5153053"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VLI90_17410"
     CDS             complement(<1..1236)
                     /locus_tag="VLI90_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16043.1"
                     /translation="MLPKIERLENRQLMSAGPALTLASSDALPFTNRLIFNRIQNQNP
                     LLGDLMHDSAIVRLINTGDQPLTIQQLSLSSSDWQFAGDGGAPMTIGAGQEVHVQLNF
                     VATTLPAVPYNETAGKVTSGGGTYTGTLTINSNDAAHPTQSLQLAGYWQKDSEHGEEP
                     SLQTIVNLIGGYKTNIASGVRQTLSQGSTTPTYYGEEIAAPYWMAANPNQPVNIQQIA
                     SWHVQGRSTTLSFYDKGDSADPTTLLTTLNDAGQELFPEATPDYRTSFTPAGAFGLTV
                     DGEFSDDTLNTSGGGGGHHFRFYPLRDANNNLIPNSFLVAQDYGGEIANFDFQDGVFI
                     LTNARPEAKLAVPANLTATVQPSLGISLQWSANVEPNLAGYNVYRGTSTAVATDGPPL
                     NGSVPQAGTSYVDRAASNGI"
     gene            1516..1692
                     /locus_tag="VLI90_17415"
     CDS             1516..1692
                     /locus_tag="VLI90_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16044.1"
                     /translation="MQLDLWWLLKLVVVTIALAIALDRALERRRRQRDRRSDSLRHAH
                     LVEHFEQPGKRRTS"
     gene            1689..2084
                     /locus_tag="VLI90_17420"
     CDS             1689..2084
                     /locus_tag="VLI90_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16045.1"
                     /translation="MIELRGRHNTLRLSPTVWTMTLELARLSGWQPAGTELGDETEDG
                     EPLVGWLMQYTSSNGQFVSAADAERLSVALDRALSPDNVAIAHRSIQDCDIALHTPPG
                     GFEWFATEAGRAHLRALGRFCGTGPFAVL"
     gene            2446..3147
                     /locus_tag="VLI90_17425"
     CDS             2446..3147
                     /locus_tag="VLI90_17425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSV16046.1"
                     /translation="MRILLVDDHQILRQGLLNLLHQHQDLKVVGQAGDGRSAVQMARE
                     LVPDVVVMDIAMPGLNGIDATRQLALAHPAAKVIALSMHVDRRFAEEMLRAGAKAYLV
                     KDGAFEELASAIRTVLEGRIYLSPRVAGGEMDQFLHRDGGGKGDGGDGDGRAGASPAR
                     SAPSAFARLSPREREVLQLMSEGRATKEIATDLGVSVKTIETHRRQLMEKLNLYSVAE
                     LTKYAVREGLTTLDN"
     gene            3315..3623
                     /locus_tag="VLI90_17430"
     CDS             3315..3623
                     /locus_tag="VLI90_17430"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16047.1"
                     /translation="MSLMTLEHFAALEVYNFVVVCDDAHEVYLLGDFNHWSTNATRMQ
                     PTEQHIWQLSLQLPPGEHRFSYFVVDRRSPSGRVAPFGNTFPLPGTWGAVVRGKEHVA
                     "
     gene            3922..>5729
                     /locus_tag="VLI90_17435"
     CDS             3922..>5729
                     /locus_tag="VLI90_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16048.1"
                     /translation="MSKQTRHRRGQPLGKRARAAAVLRRASSPVPARFESLEARTLFS
                     TFTVNSTADGGAGTLRQAIFDANAQPGADQIVFSFTGGGVHTIAPTSALPAITDTLDI
                     DGTTQSGYSGTPLVALDGQFNLGNGLVIAAPNSRIAGLQIGGFLGNGVTIAAGGDGSS
                     VVASYVGTDGTNSVWNNNGIVVNAANVTIGGLAAGAGNVISGNQGDGVQFGGASNGAV
                     AGNLIGVDASGAHVLGNSNGVNVQNSTAVAITGNVISANFGNGVNVVASTGVTIAGNK
                     IGTDAGGTMSVDGFNSFGNSGNGISFGVDAFGNTVGGATAADRNVIANNNGDGVRFNT
                     GGGGALTGTNLVQGNYIGTDVTGSLALPNGNGVTVVSGSVGVTVESNVIAGNWGEGVW
                     LEDAALVQNNLIGVAADGSSPQPNWGDGVSVFSSGATAGSTITGNVIAFNYSNGVGVY
                     GGTRNAIRGNSIHDNGYLGIDLGGDGVTPNDALGHVGPNNYANFPVITNVDNAAGAVT
                     LSGSLDSTPSTSFVLEFFSSSAADPSGYGPGQTLLGSTVVTTDASGHATFTVQFSNVS
                     VGQTVVAATATDVDGNTSEFSFAQAIPAPRSLAGSVT"
BASE COUNT         1016 a         1774 c         1884 g         1055 t
ORIGIN      
        1 gatgccgttc gacgccgccc gatcgacgta gctcgtcccg gcctgcggca ccgatccatt
       61 cagaggcggt ccgtccgtgg ccaccgcggt cgatgtgccc cgatagacgt tgtagcccgc
      121 caggttcggc tcgacgttgg cggaccattg cagcgagatg ccgaggctgg gctgcaccgt
      181 cgccgtcagg ttcgccggca cggcgagctt cgcttcgggg cgggcgttgg tgaggatgaa
      241 gacgccgtcc tggaaatcga agttggcgat ctcaccgccg tagtcctggg cgaccaggaa
      301 tgagttgggg atcaggttgt tgttggcatc gcgcagagga tagaagcgga agtgatgccc
      361 accaccgccg ccgctggtgt tgagggtgtc gtcggagaat tcgccgtcaa cggtcaggcc
      421 gaacgcgccg gcgggcgtga agctggtgcg gtagtcgggc gtcgcttcag ggaacagttc
      481 ctgtcccgca tcgttcagcg tcgtcagcag cgttgtcgga tccgcgctgt cgcccttgtc
      541 gtagaacgag agtgtcgtgc tgcgcccctg cacgtgccac gaggcgatct gctgaatgtt
      601 gaccggctga ttcggattgg cggccatcca gtacggcgcg gcgatttctt cgccgtagta
      661 cgtcggcgtg gtcgagcctt gggacagcgt ttgacgtacg ccggaggcga tgttggtctt
      721 gtacccaccg atcaggttga cgatggtctg caggctcggc tcttcgccgt gctcggaatc
      781 cttctgccag taaccggcca gctgcagcga ctgcgtggga tgagcggcgt cgttgctgtt
      841 gatcgtcagc gtgccggtgt aggttccacc gccgctggtc accttgccag cagtctcgtt
      901 gtacggcacg gccggcaacg tcgtggcaac gaagttcagc tgaacgtgga cttcctgtcc
      961 cgcgccgatt gtcatcggcg cgccaccgtc gccagcgaac tgccagtcac tgctgcttag
     1021 ggacaactgt tggatcgtca acggctgatc gccggtgttg atcaggcgga cgatcgcgct
     1081 gtcgtgcatc agatcgccca gcagcgggtt ctggttctgg atgcggttga agatcaggcg
     1141 gttggtgaag ggcagcgcat cgctgctggc gagcgtcaac gcggggccgg ccgacatcag
     1201 ttggcggttc tccagccgct caattttggg gagcatcaaa cggcgagtgg ttgattgcga
     1261 tcgcgggcgg cggtccttcg gcacaaatct ccttgtaaat cttgcagccc cgcacgaagg
     1321 cgggagacga aacatacccc gcgggtgcgg tcgcggccag ttttccaaaa tcgccgtaag
     1381 acggggtgaa tgtgcagttt atgccgtcca gcgaatggtc gccttcttgc attccttagg
     1441 tagtggctgt acatttgata cgtacaatga cgtagcagcg gctgcagtat aatgcggggg
     1501 ctcggagcga gcagcgtgca gctggatttg tggtggttgc tgaagctggt ggtagtcacc
     1561 atcgcgctgg cgatcgcatt ggatcgagcg ctggaacgcc gacgccgcca gcgcgaccgg
     1621 cggtccgata gcttgcggca cgcgcacttg gtcgaacatt tcgaacagcc ggggaagagg
     1681 cgaacgtcat gatcgaactt cgcggacgcc ataacacgct gcgactgagc ccgaccgtct
     1741 ggacgatgac gttggagctg gcgcgccttt cgggctggca accggcgggg acggagttgg
     1801 gcgacgagac ggaagacggc gaaccgctgg tgggctggtt gatgcagtac acgagtagca
     1861 atggccagtt cgtcagcgct gccgacgccg agcgtttgtc ggtcgcgctc gatcgggcgc
     1921 tgagcccgga caacgtggcg attgcccacc gctcgatcca ggattgtgat atcgcactgc
     1981 acacgccgcc ggggggattc gagtggttcg ctaccgaagc ggggagagcg caccttcgcg
     2041 ccttgggtcg gttctgcggc acgggacctt tcgccgtttt gtagctgcga cgcctgcgcc
     2101 gccttggcac gagtgaaggc gatgcaggcg tcgcagctcc attcaacgga tgagttcgct
     2161 cgcgccagcg ccacttcgcg agcaaaatgg gtctagtcac gtccgccttc gtgattcata
     2221 atcgttgcgg ttcgtggttc tcctctttgg caatcgcacg ccgtttgcta aatacaggtt
     2281 gaacgatccg accgacatga ccagcttcgc cgaccgccgt tcgtggtcaa aggttgtaca
     2341 cggtagttgg cgggctattt gagggagttg gccatgcttt ccgcaagtcg aacggtccgt
     2401 gctgcaagca gcacacaacc caaccccgct gtaacaaacg gggccgtgag aattctgctc
     2461 gttgacgatc accagatcct gcgccagggg cttctgaatt tgctgcatca acatcaggac
     2521 ctgaaggtcg tcggccaagc cggcgacggt cgatccgccg tgcaaatggc ccgcgagttg
     2581 gtgcccgatg tcgtcgtgat ggacatcgcg atgcccggcc tgaatggcat tgacgccacg
     2641 cggcaacttg cgttggcgca cccggccgcc aaggtcatcg cgctctcgat gcacgtggac
     2701 cggcggttcg ccgaggaaat gcttcgcgcg ggcgctaagg cttacctcgt taaagatggg
     2761 gcgttcgaag aactcgcgtc ggcgattcgc acggtgctcg aaggccggat ttacctcagc
     2821 ccgcgcgtcg ccggtgggga aatggatcaa tttctgcatc gcgacggtgg cggcaagggc
     2881 gacggtggcg acggcgacgg acgggcgggc gcttcgcccg cgcgctccgc gccgtccgcg
     2941 ttcgcccgcc tgtccccgcg cgagcgcgag gtgcttcagc tgatgtccga aggccgggcg
     3001 actaaggaaa ttgccaccga cctgggcgtg agcgtgaaga cgatcgaaac ccatcgacgg
     3061 cagttgatgg aaaagctgaa cctgtacagc gtggcggagc tgaccaaata cgccgtccgc
     3121 gaagggttga cgactctaga taactagcgg catgcccggg attttttggg gatatcttcc
     3181 tgcacaacct gttcaatttc cgctttgtca ggaaatcgcg gacggacatg gcgtaaactc
     3241 cgcaccggca agaacgcatc cggtgagatg tagccaagga tcacgctaac gcccgcgggc
     3301 aagggattgc cgccatgtct ttgatgacgc tggaacattt cgccgcgctc gaagtctaca
     3361 actttgtggt cgtctgcgac gacgcccacg aggtttacct gttgggcgat ttcaaccatt
     3421 ggtccaccaa cgcgacgcgg atgcagccga ccgagcagca catttggcag ctgagccttc
     3481 agctgccgcc gggggaacat cgattcagtt atttcgtcgt cgatcgccgg tcaccttcgg
     3541 gccgcgtcgc gccgttcggc aacacgttcc cgctgccagg cacgtggggc gccgtggtgc
     3601 gtggcaaaga gcatgtggcg tgacagcgcg aactcctccc gtttagcggc cgcgtctacg
     3661 cggcgcgttt cctttcccct cgaaaaccga aatacttgaa gaccaaaaaa aacgcggcgc
     3721 gtaagcgcgg ccgctaaaac ggtggcgcat gcgcgcacgg attgcacgcg caaatcgcgc
     3781 cgccccacat ccgcggcgcc gacgaatcgc ctgcgccgct tattcggttc cgtccgtaac
     3841 gcctgggcaa ccgcgccggt ttagaggagc gcggccaacc cggggctttg catttggtgc
     3901 ggagaattgg atatccgcag catgagcaag cagactcgcc atcgccgtgg ccagccgctc
     3961 gggaaacgcg cccgcgccgc tgccgtgctt cgccgggctt cgtcgcctgt cccggcgcga
     4021 ttcgaatcgc tggaagcgcg cacgctgttc tcgaccttca cggtgaacag caccgccgac
     4081 ggcggggccg gtacgttgcg gcaggcgatc ttcgacgcca acgcgcaacc cggcgctgac
     4141 cagattgtct tcagcttcac cggtggcggc gttcatacga tcgcgccaac gtcggcgctg
     4201 ccggcgatca ccgacacgct cgacatcgac ggcaccacgc aaagcggata cagcggcacc
     4261 ccgctggtgg cgctcgacgg acagttcaat ctcggcaacg gccttgtaat cgccgcgccg
     4321 aatagccgga tcgccggttt gcagatcggc gggttccttg gcaatggtgt gacgatcgcc
     4381 gccggtggcg atggcagcag cgtcgtcgcc agctacgtcg gcaccgacgg cacgaattcc
     4441 gtctggaaca acaatggcat cgtcgtcaac gcggcgaatg tgacgatcgg cggcctcgcc
     4501 gccggcgcgg gcaacgtcat ctccggcaac cagggcgacg gcgttcagtt cggcggcgcc
     4561 agcaacggcg ccgtcgccgg caacttgatc ggcgtggatg ccagcggcgc gcacgtcctc
     4621 ggcaactcca acggcgtgaa cgtccagaac agcaccgctg tcgccatcac cggcaacgtg
     4681 atctccgcca acttcggcaa cggcgtgaac gtcgtcgctt cgaccggcgt gacgatcgcc
     4741 ggcaacaaga tcggcaccga cgccggcggc acgatgtcgg tcgatggctt caacagcttc
     4801 ggcaatagcg gcaacggtat ttcgttcggc gttgacgcgt tcggcaacac cgtcggcggg
     4861 gcgaccgcgg cggaccgcaa cgtcatcgcc aacaacaacg gcgacggcgt ccgcttcaac
     4921 accggcggcg gcggcgcgct caccggcacg aacctggtcc aaggcaacta catcggcacc
     4981 gacgtgaccg gcagtctcgc gctgcccaac ggcaacggcg tgaccgtcgt ctccggctcg
     5041 gtcggcgtga ccgtcgagag caacgtcatc gccggcaact ggggcgaagg cgtttggctg
     5101 gaagatgcgg cgctcgtgca gaacaacctg atcggcgtcg ccgccgatgg ctcgtcgccg
     5161 cagcccaact ggggtgatgg cgtcagcgtc ttcagctccg gcgccacggc gggcagcacg
     5221 atcaccggca acgtcatcgc cttcaactac agcaacggcg taggcgttta cggcggcacg
     5281 cgcaacgcca tccgcggcaa cagcattcac gacaacggct atctcggcat cgatctcggc
     5341 ggcgatggtg tgacgccaaa cgatgcgctg ggccacgttg ggccgaacaa ctacgcgaac
     5401 tttccggtca tcaccaacgt ggacaacgcc gcgggcgcgg tgacgttgag cggctcgctc
     5461 gactcgacgc catcgacgtc gttcgtgctg gagttcttct ccagcagcgc tgccgatccc
     5521 agtggctacg ggccggggca gacgttgctc ggatccaccg tcgtcacgac cgatgcatcg
     5581 ggccacgcga cgttcacggt gcagttctcg aacgtctccg ttggacaaac cgtcgtcgcc
     5641 gcgacggcga cggacgtgga tgggaatacg tcggagttca gcttcgcgca agcgattccc
     5701 gcgccgcgct cgctggcggg gtccgtcac
//
LOCUS       DATIMH010001000         4876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_528529, whole genome shotgun sequence.
ACCESSION   DATIMH010001000 DATIMH010000000
VERSION     DATIMH010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528529"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VLI90_17440"
     CDS             complement(<1..291)
                     /locus_tag="VLI90_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16049.1"
                     /translation="MPKRLVFSALTLFAAQTAGRNMTKAAYVAVTVGVSVMVLLTVVP
                     AYEAAHLWVDAVLWVCWAYFLFEWVVRLRHARALERLWGYALSFRGLVDAAAV"
     gene            606..1268
                     /locus_tag="VLI90_17445"
     CDS             606..1268
                     /locus_tag="VLI90_17445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSV16050.1"
                     /translation="MDTSHLAEHAGTAGALFASIFVVATTTMRTMIPLRIFGILANVV
                     LFLTAIPAHNYLVMLVQSVMCLVNSYRLHQMLQLVRDVKKSVNSDLSMDWLKPFMTER
                     NCAAGEMLFYKDEKAEDMLYIVSGKFKLVESGIELPVGAIVGELGMLSPSNVRTQSLE
                     CIEAGLVLSVSYTKVEELYVQNPAFGFYFLRLSSARLFQNLETLQKQLAQQIAAAQPK
                     PA"
     gene            1287..2144
                     /locus_tag="VLI90_17450"
     CDS             1287..2144
                     /locus_tag="VLI90_17450"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HSV16051.1"
                     /translation="MSDRGILSSLRYLFADVIGDDDAPTPFLPEGLPEAVVSVASEAI
                     HMLIEYQGPSYAQLYVDRLKRFIGRRGFDDAVFAEVARLLAARMSYEDPIRIAQLTLA
                     ALDERPVPVNEVKKFRIDELVGALPAIIAEPVLDTLEWLGWLRLPVSIRFNAASRLGI
                     RRLKIEAGLKRWRLFSVRYPKERAWVERWLHMIDRSLTKQPNAAPAIVHTADMISGYG
                     DPYRQGMADWNVIMDGLAKPTFDGVLDLADLGSAVTEARAAVLPDPRQAALKRKIAEI
                     RARAPAETT"
     gene            complement(2148..3590)
                     /locus_tag="VLI90_17455"
     CDS             complement(2148..3590)
                     /locus_tag="VLI90_17455"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HSV16052.1"
                     /translation="MSAEAPTPQPEMPRRRSRVVKSNRTQIVLFSVLTLILTAVTVWG
                     GRTWLRNSETLVFAVGEANGPEARFAAKLAALLKSNTSRLRLKILPHGDNTKALAAFD
                     RKEANLAILRTDGKVPSRARAVAILDHDVLLLLSPGDKKIKTVGDLKKKKIAVMADSE
                     NSATLVRNILELSDSPDAAARVQLAPPGATLEKLFASGNGAVVVIAHASQIMKDRSYE
                     QLAKRGGFTLNAIDAAKALTRKYPALSEETLTTGMLSAAPAIPDDDLTTLGLEWLLVA
                     QSRLSTTTMSDLARLIYENKADLALSDGFASKIEPAVTDKDAFIAAHAGAAEYINDDT
                     KSFMDRYSDMMYLGAAALSVIGSIFAGIYTKVTRVAPEKASELATAILDIGERMEYAK
                     TLDALDELQDELEAILRGAVIGLRDGTISSDGLETFKLGYEFVRDEIGMRRDHLRRHP
                     SPNPGTQASATPTAVAHDDNVVVVKTAHSA"
     gene            4001..4354
                     /locus_tag="VLI90_17460"
     CDS             4001..4354
                     /locus_tag="VLI90_17460"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HSV16053.1"
                     /translation="MLLLTVKHVIADFVLQNSWIAIGKDQKTGWALPLLAHCLVHLAV
                     SMALILVVAPRFWFVALIDFAIHITVDRLKGICASSFGVTLEQEHPWFWTLIGVDQAL
                     HHLTGFGLSIFMASN"
     gene            4543..>4876
                     /locus_tag="VLI90_17465"
     CDS             4543..>4876
                     /locus_tag="VLI90_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16054.1"
                     /translation="MFSSRDAFENDFCPVRDELLGEMYRANANGLPMLVESVSSEVRA
                     MLALFCYRRSHLHALAVAIAASCTERELIQLGGRVGSTLYALSREPAARSAPSSAYGH
                     RKPITLSTK"
BASE COUNT          863 a         1526 c         1531 g          956 t
ORIGIN      
        1 gacggcggcg gcgtccacca gaccgcggaa ggacagcgca taaccccaca atcgctccaa
       61 cgcccgcgcg tgacgcagtc gcaccaccca ttcgaacagg aagtaggccc agcataccca
      121 cagcacggca tcgacccaga gatgcgccgc ctcataggcg ggcacgaccg tgagcagcac
      181 catgacgctg acgcccaccg tgaccgcgac ataggccgcc ttggtcatgt tgcggccagc
      241 cgtctgggcg gcgaaaagcg tcagagccga aaaaacgagc cgtttgggca ttggaaaaag
      301 ggcgcctgat cggtactgaa aaagcgtggg tgcggtcatg ccacggcggg accgctccgc
      361 gcaacgtgcc cgcctccggg gaggccgtca acgacatcag gcaaacaccg tttatcgccg
      421 tcggccgggg gcgccacgga agcttggtag ctccccggga gccggatttt gcccgggttt
      481 gacgaaaatc ccgataaaac gccgcttttc ctcgtatatc aaaaacggcc cgcggccatt
      541 acgatagcgg gcactgcagc agaatcgcaa atccgtttca ctttccaaac aagtggttgg
      601 ctccaatgga tacgtctcat ctggcagaac atgcgggaac cgccggcgcg ctgttcgcgt
      661 cgatcttcgt ggtcgccacc accacgatgc ggaccatgat cccgttgcgg atcttcggca
      721 ttctcgccaa cgtcgtgctc ttcctgaccg ccattccggc gcacaattac ctggtgatgc
      781 tggtgcagtc ggtgatgtgc ctcgtcaact cgtatcgcct gcaccagatg ctgcaactgg
      841 tgcgcgacgt gaagaagtcc gtcaacagcg acctgtcgat ggactggctg aagccgttca
      901 tgaccgagcg caactgcgct gccggcgaaa tgctattcta caaggacgag aaggccgagg
      961 acatgctcta tatcgtcagc ggcaaattca agctggtcga gtcgggcatc gagctgccgg
     1021 tcggcgccat cgtcggtgaa ctcggcatgc tgtcgccctc gaacgtgcgt acccagtcgc
     1081 tggaatgcat cgaggccggc ctggtgctga gcgtcagcta caccaaggtc gaggagctct
     1141 atgtacagaa cccggcgttc ggcttctatt ttctccgcct ctccagcgcc cggctgtttc
     1201 agaacctcga gacgctgcag aagcaactcg cgcagcagat cgccgcggcc cagccgaagc
     1261 cggcctagag gccgggagca acgggcgtgt ccgaccgcgg tatcctttcc tcgttgcgtt
     1321 acctgtttgc cgatgtcatc ggcgacgacg acgctccgac gccgttcctc cccgagggat
     1381 tgccggaagc cgtagtgtcc gtcgccagcg aagccattca catgctgatc gagtatcagg
     1441 ggccgagtta cgcccagctc tatgtcgatc gcttgaagcg gttcatcggg cggcgcggtt
     1501 tcgacgatgc cgtctttgcc gaggtcgcgc ggctgttggc ggcgcggatg agctatgaag
     1561 atccgattcg gatcgcgcag ctcacgctcg ccgcgctcga tgagcggccg gtgccggtca
     1621 acgaggtcaa gaaattccgc atcgatgaat tggtcggcgc gttgcccgcc atcatcgccg
     1681 agccggtgct cgatacgctg gagtggctgg gctggctgcg gctgccggtg tcgatccgct
     1741 tcaacgcggc gagccggttg ggtattcgcc gcctgaagat cgaggccggg ctaaagcggt
     1801 ggcggctgtt ttcggtgcgt taccccaagg agcgggcctg ggtcgaacgc tggctgcaca
     1861 tgatcgaccg cagcctgacc aaacagccga atgctgcgcc cgccatcgtg cacaccgctg
     1921 acatgatcag cggatacggc gatccctatc gtcagggcat ggcggactgg aatgtcatca
     1981 tggacggcct cgccaagccg accttcgacg gtgtgctgga tctggccgat ctcggtagtg
     2041 cagtgacgga agcgcgcgcc gcggtgctgc ccgatccacg gcaggcagcg ctcaaacgca
     2101 agatcgccga gatcagggcg cgggcgccag ccgaaactac ctgaacctta tgcgctgtgc
     2161 gcggtcttca cgaccacgac gttgtcgtca tgcgcgacgg cggtcggggt cgccgaagct
     2221 tgcgtgccgg gattcggcga ggggtgccgc cgcagatggt cgcggcgcat gccgatctcg
     2281 tcgcggacga attcataacc gagcttgaag gtctccagtc cgtcgctgga aatagtgccg
     2341 tcacgcaggc cgatcaccgc gccgcgcagg atcgcctcga gctcgtcctg caactcgtcg
     2401 agggcatcga gggtcttggc gtattccatc cgttcgccga tatcgagaat ggcagtggcg
     2461 agctcgctgg ccttctccgg cgcgacccgc gtcactttgg tgtagatgcc ggcaaagatc
     2521 gagccgatga cgctcagcgc tgccgcgccc agatacatca tgtcgctgta gcgatccatg
     2581 aacgacttgg tgtcgtcgtt gatatattcg gcggcgccgg catgggcggc gatgaaggca
     2641 tccttgtcgg tcaccgccgg ttcgatcttg ctggcgaagc cgtcgctcag cgccagatcg
     2701 gccttgtttt cgtagatcag gcgggcgagg tcactcatgg tcgtggtcga caatctcgac
     2761 tgcgccacca gcagccattc cagcccgagg gtggtgagat cgtcgtcggg gatcgccggt
     2821 gcggcggaca gcatgccggt ggtcagcgtc tcctctgaca gcgccgggta ttttcgggtc
     2881 agcgctttgg ccgcgtcgat cgcgttcagg gtaaaaccgc cgcgcttggc caattgttcg
     2941 tagctcctgt ccttcatgat ttgcgaggcg tgcgcgatca cgaccacggc gccgttgccc
     3001 gaggcgaaca gcttctccaa ggtcgcgccc gggggcgcca actggacccg cgccgcggcg
     3061 tcggggctgt cggaaagttc gaggatattg cggaccaggg tcgcgctgtt ctcgctgtcg
     3121 gccatgaccg cgatcttctt tttcttcagg tcgcccacgg tcttgatctt cttgtcgccg
     3181 ggactgagca gcagcagcac gtcgtggtcg aggatcgcga ccgcgcgggc gcgcgacggc
     3241 accttgccgt cggtgcgcag gatcgccagg ttggcttcct tgcgatcgaa cgccgccagt
     3301 gccttggtgt tgtcgccgtg gggcaagatc ttgagccgca gccgcgacgt attgctcttc
     3361 agcagcgccg cgagtttggc ggcgaagcgc gcctcgggac cgttggcctc accgacggca
     3421 aacacaagcg tctcggaatt ccgcagccag gttcggccgc cccacaccgt gacggcggtc
     3481 agaatcaagg tgagaaccga gaacagcacg atctgcgtgc gattgctttt caccacccgc
     3541 gaacgtcggc gtggcatttc cggttgcggc gtgggggctt cagcgctcat ggctcatacc
     3601 cggtcgggga cgtctcgccg tcaaaccggc gtcgcaggcc gtcctaatga tttcgtaaat
     3661 acctagaaac gaacgacaat tttgcctgtg ggcgatgata tcgcgcttcc aacggaatgc
     3721 aaaccgcccc ggcgggtaac cttaccgcga ggacggtgcg cggagcggta tcgcagttca
     3781 gccacaccaa gcgatttttt ggactaccca caggtgcatt ccggttcggg aggtgtcata
     3841 aatgtcgcca acgtttcgga cggtccgggt ttgagaagag aattgcgctg actgcctaga
     3901 tcacggtttt tcgccacctc gtcagtccca gcaaagggcg tcagctttgt tgtttccttc
     3961 gatgtcgtcg tcggtcccgg tgggtgcgct gataggatgg atgttgcttc tcaccgtgaa
     4021 gcacgtcatc gccgatttcg ttttgcagaa ctcctggatt gcgatcggca aggatcagaa
     4081 gaccggctgg gcgctgccgc tgctggcgca ttgtctggtg catctcgccg tgtcgatggc
     4141 gctgatcctg gtggtcgcgc cgcggttctg gttcgtcgct ttgatcgact tcgccatcca
     4201 catcacggtc gaccggctca agggcatctg cgcctcgtcg ttcggcgtga cgctggagca
     4261 ggagcatccc tggttctgga cgctgatcgg cgttgaccaa gcgctgcacc atctcaccgg
     4321 attcggcctg tcgatcttca tggcgtcgaa ctgaccgctg tccggccagg gcagggctcc
     4381 tgacccaaac ctggaccagt ctccacccct ctgcagccga atcacggttg cggccggctc
     4441 cgattcggcc tcaaccggca agtgccccgg gacactactg cctccgttgg ttaacgtttc
     4501 attagagaat tgcgatgcat cttccgcata gggagaaccc tcatgttttc aagccgtgac
     4561 gcattcgaga acgatttttg tccggtcagg gatgaactgc tcggcgagat gtaccgcgcc
     4621 aacgccaacg gcctgccgat gctggtcgaa agcgtgagct cggaagtccg cgcgatgctg
     4681 gcgctgttct gctatcgtcg cagccatctg cacgcgctgg ccgtggccat cgcggcaagc
     4741 tgcaccgaac gcgaactgat ccagttgggc ggccgcgtcg gctccaccct gtacgcgctg
     4801 tcgcgcgaac cggccgcacg ctccgcaccc tcgtcagcct acggccatcg caagccgatt
     4861 acgctgtcga ccaaac
//
LOCUS       DATIMH010001001         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4863170, whole genome shotgun sequence.
ACCESSION   DATIMH010001001 DATIMH010000000
VERSION     DATIMH010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4863170"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /gene="pyrF"
                     /locus_tag="VLI90_17470"
     CDS             complement(<1..721)
                     /gene="pyrF"
                     /locus_tag="VLI90_17470"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSV16055.1"
                     /translation="MSDHFADSLLDAIAAKGSPICVGIDPMYDQLPDAIAGQPADRDG
                     NDPEAAIDAIFAFTTTVLRTIAPHVPIVKFQSAYFEKYLWEGVESYYSLIQEAKDLGL
                     LVIGDVKRGDIGSTSAAYAAAHLAESPFAELEAQVPDAITINPMLGIDSIEPFVNVAK
                     SEGKGLFALVRTSNPGSADFQDAALADGRTWSELVADKLAVVAADPALVGSQGYSSLG
                     AVVGATQTHTMQSLRRRLPRSI"
     gene            complement(881..1327)
                     /locus_tag="VLI90_17475"
     CDS             complement(881..1327)
                     /locus_tag="VLI90_17475"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HSV16056.1"
                     /translation="MREALTDAFRLALQSAQSSARELNQDFVGTEHLMLGLLGIDGEA
                     ARTMKAAHGDPEQVKSALSAALPRGEEPPVVTGDLPLSPKAQRIVNGAIVKAQAMRET
                     RVSTRFLLLSLLDEPDTAVREAMNKAGTDLETLQRKLAEKPTMAEA"
     gene            complement(1561..2028)
                     /locus_tag="VLI90_17480"
     CDS             complement(1561..2028)
                     /locus_tag="VLI90_17480"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSV16057.1"
                     /translation="MSLETQPTAKPADLCTCSHDSGVAVWKQLLVTYLKMQRRVEEML
                     TPYGLVLSQFEALAKIGLKPGIIQQELVAVLLVTKGNVGALLDRLESIGLVERQSDPN
                     DRRANRLHLTQPGEQMVAEIFQKHCALVREMLQPLDPQQRQTLQSLLKMLEPA"
     gene            complement(2170..>3056)
                     /locus_tag="VLI90_17485"
     CDS             complement(2170..>3056)
                     /locus_tag="VLI90_17485"
                     /inference="COORDINATES: protein motif:HMM:NF045031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16058.1"
                     /translation="FKALREQFIKNLHIDPPPIPADPKILNLPAIELSRQCALLDTVP
                     GVRDSAWSARTPITSEDVLTMEDVDQAYGFILYRKLFENGVKGELKLGKALDYSIVMI
                     DGQPVGRAFAGYGPQTFNMKVDHAGPCTLEILVYNLGRNSVGINQSISRKGLNEDPTL
                     DGKRLQGWQIFPLPLASPGELPAVKTKSGPGPAFHRGTFTLNETGETYLDMRNWGFGV
                     VWVNGHNLGRYWDVGACRSLYLPSVWQKQGENEIVILELNDGAPKREQVTGATQLIVE
                     PAVKFPLDAERDKKVTPE"
BASE COUNT          537 a          963 c          946 g          610 t
ORIGIN      
        1 agatcgaccg cggcaaccgc cgccgcaggc tttgcatcgt gtgcgtttgg gtcgcgccga
       61 cgacggcgcc aaggctgctg tatccctgcg aaccgactag cgccggatcc gccgcgacca
      121 ccgccagctt gtccgccaca agttctgacc atgtccggcc atccgctagc gcggcatctt
      181 gaaaatccgc cgagcctgga ttgctcgtgc gcactagcgc gaacaatccc tttccctcgg
      241 actttgcgac gttgacgaac ggctcgatcg aatcgatgcc cagcatcggg ttgatggtga
      301 tcgcatccgg cacctgcgct tccagctcgg cgaacggcga ctccgccagg tgcgccgccg
      361 cgtatgcggc actggtcgaa ccgatgtcgc cgcgcttcac gtcgccgatc accagcaggc
      421 ccaggtcctt cgcctcctgg atcaggctgt agtacgactc gacgccttcc cacagatatt
      481 tctcgaagta cgccgattga aacttcacga tcggcacatg cggcgcgatc gtgcggagca
      541 cggtggtggt gaacgcgaag atcgcgtcga tggccgcctc cggatcgttc ccgtcccgat
      601 cggccggttg cccggcgatc gcatccggca gctggtcgta catcgggtcg atgccgacgc
      661 aaattggcga acccttggcg gcaatggcgt cgagtaaact gtcggcgaaa tgatcggaca
      721 tgtgtggatc ggtttccgtt aaagcgtcgg gaacaagcga agattgagac caatagccca
      781 ggccggaacg ccggggatcg gactgcgagt gatccgattt tgaaattcga tccctggcct
      841 tccggcctgg gctatcaaag actcgcagcg cgcacgcgtc tcacgcttca gccatcgtcg
      901 gtttctcagc cagcttccgc tgcagcgtct ccaggtccgt ccccgccttg ttcatcgctt
      961 cgcgcacggc ggtatccggc tcgtccagca gcgacagcag caggaaccgt gtcgataccc
     1021 gcgtctcccg catcgcctgg gccttcacga tcgccccatt gacgatgcgc tgcgccttag
     1081 gcgacagcgg caggtcgccc gtgacgactg gcggctcttc gccgcgggga agcgcggcgc
     1141 tcagcgccga cttcacctgc tcgggatcac cgtgcgccgc tttcatcgtc cgggcggctt
     1201 caccgtcgat gccgagcagg ccgagcatca ggtgttcggt cccgacgaaa tcctggttca
     1261 gctcgcgggc cgaggattgc gccgactgca gcgcgagacg aaaggcatcc gtaagcgctt
     1321 cacgcatcgc agggcctccg cggttgatgc tcgggcttga acaacggctg agtcatgccg
     1381 cgcgatgata gccggaaggg gtggatgtgt cacagggaac gcccatgttg cgatggaggt
     1441 tggaaattga acgcagagac gcagaggccg cagagaacgg aattaagctt ctactccgct
     1501 cctaacgttg cggcctctgc gtctctgcgt tcaatgattt ctgtcgtgcg tgccgtgtgc
     1561 tcacgcgggc tcgagcatct tcaggaggga ttgcagcgtc tggcgttgct gcgggtcgag
     1621 cggttgaagc atttcgcgaa cgagcgcgca gtgcttctgg aaaatctccg cgaccatctg
     1681 ctcgccgggt tgcgtgaggt gcagccggtt ggcccggcgg tcgttcggat cggactggcg
     1741 ttcgaccagg ccgattgatt ccagccgatc gagcagggcg ccgacgttcc ccttggtcac
     1801 cagcagcacg gccaccagtt cctgctggat gatgccgggc ttaagcccga tcttcgccag
     1861 cgcttcgaac tgcgacagga ccagcccgta cggcgtcagc atctcttcga cgcggcgctg
     1921 catcttcaga tacgtgacca gcaactgctt ccaaacggcc acgccgctgt cgtggctgca
     1981 ggtacacaaa tcagccggtt ttgcggtggg ttgggtttcc aaggacattt agtcaaatat
     2041 tagacagttg ttgcggtggg gtcaatttca ggagatcgag atcgattgcc cagctgacaa
     2101 ttagtagcac gggcgtctcg cccgtgccga agtgttcagg cacgggcgag acgcccgtgc
     2161 tacgaattgt cactccggcg tgaccttctt gtcgcgctcg gcgtccaaag ggaacttcac
     2221 cgcgggctcg acgatcagct gcgttgcgcc cgtcacctgc tcgcgtttgg gcgcgccgtc
     2281 gttcagttcc agaatgacaa tctcgttctc gccttgcttc tgccagacgc ttggcaggta
     2341 tagcgatcgg caagcgccga cgtcccaata gcggccgagg ttgtggccgt tgacccagac
     2401 gacgccgaag ccccagttgc gcatatccag gtatgtctcg ccggtctcgt tcagcgtgaa
     2461 cgtcccgcgg tgaaatgcgg ggccggggcc cgatttggtc ttcaccgccg gcaactcgcc
     2521 gggggaagcc agcggcaggg ggaagatttg ccagccttgc agccgcttcc catccagcgt
     2581 gggatcctcg ttcaagccct ttcggctgat cgactgattg atgcccacgc tgttgcggcc
     2641 gaggttgtac acgaggatct ccagcgtgca cggccccgcg tgatcaacct tcatgttgaa
     2701 cgtctgcgga ccatatcccg cgaacgcgcg accgacgggt tggccgtcga tcatcacgat
     2761 cgaataatcc agcgccttgc cgagcttcag ctcgcccttc acgccgttct cgaacagctt
     2821 gcgatagagg atgaagccat acgcttggtc gacgtcttcc atcgtcaaga catcttcaga
     2881 cgtaatcggc gtgcgagcgg accaagccga atcgcgcacg ccgggaacgg tgtcgagcag
     2941 cgcgcattgc cgcgacagct cgatcgccgg caggttcagg atcttcggat ccgccgggat
     3001 cggcgggggg tcgatgtgca ggttcttgat gaactgctcg cgcagcgctt tgaact
//
LOCUS       DATIMH010001002         1665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2548797, whole genome shotgun sequence.
ACCESSION   DATIMH010001002 DATIMH010000000
VERSION     DATIMH010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2548797"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..820
                     /locus_tag="VLI90_17490"
     CDS             173..820
                     /locus_tag="VLI90_17490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV16059.1"
                     /translation="MPEPIASSAEPAKAGNDPDLRPSDGPLVQRVIAGDRGAFDELIR
                     RYQRQAVAVSYRLLGSSHDALEVTQDAFLKAYKSIDTLQKPDAFGGWLMRIVSNLSLN
                     FRRSRKSRSQLPLDDILGPSDAGQAEGSGGSEWMARSGDPVRGLESREMGQRLQEALK
                     QLPEKQRLAIVMFTIDEMPQKDVAAALDCSVEAVKWHVFQGRKKLKELLKEHLQE"
     gene            883..1479
                     /locus_tag="VLI90_17495"
     CDS             883..1479
                     /locus_tag="VLI90_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16060.1"
                     /translation="MISEELEFQISQYADGTLPAAERAALEVRLAEDAEARRMVAQYR
                     RLGEHLSRDLGPLPNVKWDRLAAHLSSAIAAEDALAVIGRIGSASGAATWTWRSRFAI
                     AAGVLVALGTGLLVYNGHGGNPAPAPVEPTAVASVSGPQAEPAAGPAVQEIKIGPSPA
                     LAKSADSWRYAEGVVQRPSSAVIAGEMKPPHEGESFIH"
BASE COUNT          323 a          474 c          563 g          305 t
ORIGIN      
        1 tttgccgcta gagctggccg acaacgggta acaggattga cccctcctgc ctctgcggcc
       61 tttgcgtctc tgcgttcaat tgatgttttc gcctcgcaaa aatccaactc gcgtctaacc
      121 tcagactgta taaagtgtca catctatgga gcgcggtggc agcgatgcga atatgccaga
      181 gcctatcgca tcgtcggcgg agccggctaa ggctggaaat gacccggact tacggccgag
      241 tgacggtccg ttggtccagc gggtaattgc cggtgaccgc ggggcgtttg acgagctgat
      301 ccggcgttac cagcgtcagg cggtggccgt gtcgtatcga ctgctgggga gcagccacga
      361 cgccttggaa gtcacacaag atgctttcct gaaggcgtat aagagcatcg acacactgca
      421 aaagcccgac gccttcggcg gttggttgat gcggatcgtg tcgaacctgt cgctcaactt
      481 caggcggagc cgaaaaagcc ggtcgcaact gccgctggac gacatcctgg gaccgtcgga
      541 tgcggggcaa gcggaaggtt cgggcggcag tgaatggatg gcccggtccg gtgatccggt
      601 gcgaggcttg gaaagccggg agatgggcca gcggctgcag gaagcgctga agcagctgcc
      661 ggagaaacag cggctggcga tcgtgatgtt cacgatcgac gaaatgccgc agaaggatgt
      721 ggccgcggca ctggattgca gcgtggaagc ggtgaagtgg cacgtgtttc agggacgaaa
      781 gaagttgaag gagttgttga aggagcattt gcaagagtag cacgggcgtc tcgcccgtgc
      841 cgagtcattt agacacgggc tagacacccg tgccacaaaa tcatgatcag cgaagaactc
      901 gaatttcaga ttagccaata cgccgacggc acactgccgg cggcggagcg ggctgcgctg
      961 gaagttcgac tcgctgaaga cgccgaagcg cgccggatgg tggcgcagta ccgccggctc
     1021 ggcgagcatc tgtcgcgcga cctcggtccg ctgccgaacg tgaagtggga tcgactcgca
     1081 gcgcatctgt cgagcgccat cgccgcggaa gacgcgctgg ccgtgatcgg tcgaattgga
     1141 tctgcgagcg gcgccgcgac gtggacctgg cgatcgcgct tcgcgatcgc cgcgggcgtt
     1201 ttggtcgccc tcggcaccgg gctgctcgtg tacaatgggc acggtggcaa cccagcgccc
     1261 gcgccggtcg agccaaccgc cgtcgcatcc gtcagcggtc cgcaagccga acccgcggcg
     1321 ggccccgcgg tgcaggagat taagatcggt ccgtcgcccg cgctggccaa gtcggccgac
     1381 tcctggcgct atgccgaagg cgtcgtccag cggccgtcgt ccgcggtcat cgccggcgaa
     1441 atgaaaccgc cgcatgaagg cgaatcgttc attcactgac gcgcggaaat gcagaatgat
     1501 gaatcactac tgtccggtta gtcgccggcg ttaggttggg ttgacctcgg ttttgccgag
     1561 tcgcagaact ccgatgttag ggtacacgat ttcaatttcg ttggtcgatt ttgccgtcgc
     1621 ggccaggtgg cttggccgac gatcgggcga tgaacaccgg tcgca
//
LOCUS       DATIMH010001003         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1977192, whole genome shotgun sequence.
ACCESSION   DATIMH010001003 DATIMH010000000
VERSION     DATIMH010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977192"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VLI90_17500"
     CDS             <1..1059
                     /locus_tag="VLI90_17500"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV16061.1"
                     /translation="ALLSKPTAVVTPLIAVIFDWAVYRRSPRAIARSIAPWFAVVIPM
                     ALITKYAQPASAVAGAVPLVFRPLVAGYAIAFYAKQVVLPLQLAVDYGRNPAGLYAHP
                     STALAYSAATIALLIVAWQARRRAPLLGAGILVFIAALLPNAGLVASDIQQWATVADR
                     YVYAAMLGPALLLAWALSRPVARLHALPVIVLTVSALGAFMAKAAAQAWYWHDDARLF
                     SYVVHVNPDSWIGHDHLSFGLLRTDPPRALVEAKIAQRLRPNAADPHRTLAAAYLANG
                     NHAGAIEQYRAILRFEPDDAMVMTDLAAELAESGRPDEAIPLYQSALRLSPNLHAARE
                     GLALAQAARNQSPPATQR"
     gene            1110..2693
                     /locus_tag="VLI90_17505"
     CDS             1110..2693
                     /locus_tag="VLI90_17505"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV16062.1"
                     /translation="MKAIADADRHLAPSIPSPRAAARSPTTAALIVAGVTFLVWLPCL
                     WNDFVEWDDLAMIIANPRFNPPTVRTVGWYWTHVAWNLYQPLTCTLWGVLAKVGWVNT
                     ADQFGGHLNPAVFHLAGVLLHVAAALALLSILRRAVRDDIAAIAGTLVFALHPIQTEA
                     VVFAGVLNNPLFTSLSFIAIDQYLRYTEPADSQAYNTRRRRWHLILGSIALVLAMISK
                     PTAVAVPAIAFVLDLAINHRPWRAALGSIVPWLVLILPCIAWTKRFQHGAVAAGQAPV
                     WFRPFIAGDAAAFYLWKIVYPLHLAIDYGRNPQYVFAHAWGYLTWLAPAALVVLAWRS
                     RRRRPLLAAAIAIFFIALLPNSGLLPFDFQSISTTADRYVYLAMTGVALAVAAAVAEL
                     RARHRRVVPFVIALPLLAWIVLAQFQLRTWRDGYSLYRHALALNPNSWLSLGNLAYIE
                     ADPSPEQAIADCRRAIQLNPSDAVAYNTLGSVLMQRGDHAAALDAFATAHQLEPDDAL
                     FALNYQRAVDHGRGPSSAR"
BASE COUNT          435 a         1014 c          901 g          564 t
ORIGIN      
        1 gcgctgttga gcaaaccaac cgcggttgtc acgccgttga ttgcggtgat tttcgattgg
       61 gccgtctacc gtcgatcgcc gcgcgcgatc gcacgttcga tcgcgccgtg gtttgccgtc
      121 gtcattccga tggcgctgat cacgaagtac gcccaacccg ccagcgcggt ggcgggggcg
      181 gtgccgctgg tgtttcgccc gctggtcgcg ggctatgcga ttgcgtttta cgccaagcag
      241 gtcgtgctgc cgctgcaact ggcggtggat tacggccgca accccgcagg gttgtacgca
      301 catccttcga cggcgctggc gtacagcgcg gcgacgatcg cgttgctcat cgtcgcctgg
      361 caagcgcgcc gccgtgcgcc gctgctcggg gctggcatcc tggtcttcat cgcagcgctg
      421 ctgcccaacg cggggctggt cgcctcggac atccaacaat gggccaccgt cgccgatcgc
      481 tatgtctatg ccgccatgct cggcccggcg ttgctgctgg cgtgggcact ctcgcggccg
      541 gtcgcgcgcc tccacgcgct gcccgtgatc gtgctgacgg tgtcggcgct gggcgcgttc
      601 atggccaagg ccgccgcgca ggcgtggtat tggcacgacg atgcccggct gttttcttac
      661 gtcgtccacg taaaccccga cagttggatc ggccacgatc acctgtcgtt cggcctgctg
      721 cgcacggacc cgccccgcgc gctggtcgag gcgaagatcg cgcagcggct gcgcccgaat
      781 gccgccgacc cgcatcgcac gcttgccgct gcgtacctcg ccaacggaaa tcacgccgga
      841 gcgatagagc agtaccgagc gatcctgcgg ttcgaaccgg acgatgcgat ggtcatgacg
      901 gacctcgccg ccgagctggc cgaatcgggc cggccggacg aggccatccc gctgtatcag
      961 tcggcgcttc gtctatcgcc gaatttgcat gccgcgcgcg aaggactggc actggcacaa
     1021 gccgcgcgta atcaatcgcc gccggcaacg cagcggtgat ccgcgggtcg tgccgcgtaa
     1081 gatcatctcg aatgaaactg acttaactgg tgaaagccat cgccgacgct gatcgtcatc
     1141 tcgcgccgtc catcccgtcg ccgcgcgccg cagcgcgttc gcccaccacc gccgcgctga
     1201 tcgtcgccgg cgtcacgttc ctcgtctggc tgccgtgcct gtggaatgat ttcgtcgaat
     1261 gggatgacct ggcgatgatc atcgccaacc cgcgcttcaa tccgccgacg gtgcgaactg
     1321 tcggctggta ctggacgcac gttgcgtgga acctgtatca accgctgacg tgcacgctgt
     1381 ggggcgtgct ggccaaagtg gggtgggtca acaccgcgga ccaattcggg gggcatctga
     1441 acccggcagt ctttcacctg gccggcgtgc tgctgcacgt cgcggcagcg ctggcgctgc
     1501 tgtcgatcct gcgacgcgcc gtacgcgatg acattgccgc catcgccggc acgctggtct
     1561 tcgcgctgca cccgatccaa accgaagccg tcgtctttgc cggcgttctc aacaatccgc
     1621 tgtttaccag tctctcgttc atcgcgatcg atcaatacct gcgatatacc gaacctgccg
     1681 attcacaggc gtacaacacg cgccgccgtc gctggcacct catccttggc tcaatcgcgc
     1741 tcgtgctggc gatgatctcc aaacccactg ccgtcgccgt gccggcaatc gcgttcgtgc
     1801 tcgacctggc gatcaaccat cgtccgtggc gggcggcact tggctcgatc gtcccgtggc
     1861 tggtgttgat tctgccctgc atcgcgtgga cgaaacggtt ccaacacggc gcggtggccg
     1921 ccggccaagc cccggtgtgg ttccggccgt tcatcgccgg cgacgctgcc gcgttctacc
     1981 tgtggaagat cgtttacccg ctgcaccttg cgatcgatta cggccgcaac ccgcaatatg
     2041 tcttcgcgca cgcgtgggga tatttgacgt ggctcgcgcc cgcagcactc gtcgtgctcg
     2101 cgtggcggtc gcgccgccgc cggccgctgc tcgccgctgc catcgccatc ttcttcatcg
     2161 cgctgctgcc gaactccggc ctgctgcctt tcgattttca aagcatctcg acgacggccg
     2221 accgctatgt gtacctcgcg atgaccggcg tcgccctcgc cgtcgccgcc gcagttgccg
     2281 agctgcgcgc ccgccaccgg cgcgtcgtcc cgttcgtcat cgcgctgccg ctgctggcgt
     2341 ggattgttct cgcgcagttt cagctccgca catggcgcga cggctactcg ctctaccggc
     2401 acgcgctggc attgaaccct aatagctggt tgtcgcttgg caatctcgcg tacatcgaag
     2461 cggacccgtc gcccgagcaa gcaatcgccg actgccgccg tgcaatccag ctgaacccgt
     2521 ccgacgcggt cgcgtacaac acgctcggtt ccgtcctgat gcaacgcggc gatcacgccg
     2581 cggcattgga cgccttcgcc acggcgcacc agctggagcc agatgacgcg ctcttcgcgt
     2641 tgaactacca gcgtgcggtc gatcatggcc gtggcccgtc cagcgcgcga tgatcggtgg
     2701 ttgccattac gagagcccgt cgggcgcgcg ccagctgaac tcgctaaccc gccgtgcgat
     2761 gcattccaag gcaaacgcat gtacgaatag cgcgctctga tcgatcccat ctcccggtac
     2821 gccgctgacg aaccgacact tttgcaggaa tcattgagaa cccggcgtgg gtcggtccct
     2881 tgctaaatgt gcaaaagtgt cgggccgtcg gcgg
//
LOCUS       DATIMH010001004         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5727995, whole genome shotgun sequence.
ACCESSION   DATIMH010001004 DATIMH010000000
VERSION     DATIMH010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5727995"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..764)
                     /locus_tag="VLI90_17510"
     CDS             complement(99..764)
                     /locus_tag="VLI90_17510"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HSV16063.1"
                     /translation="MVINGHLLLNTLLVVFDLGGTFVFALSGATAGVKHRLDLFGVLV
                     LSFAAGNSGGIARDVMIGAIPPAAISDWRYVGVSILAGLITFYWYRIINRLSSPVLVF
                     DAAGLALFAVSGAGKALAFHAGPVAATLLGMLTGIGGGMVRDVLVREIPTVLRTELYA
                     VAALIGAAGVVIGSMLHLSSSVAATAGAVLCFALRFMAIRHGWQLPIARPSDEPSARG
                     IRQ"
     gene            complement(771..>1680)
                     /locus_tag="VLI90_17515"
     CDS             complement(771..>1680)
                     /locus_tag="VLI90_17515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSV16064.1"
                     /translation="DMHVTWLIGAATLFAKARDKWKGTLMPVFQPAEETAAGAQAMID
                     DGLFRRFPKPDVILGQHVMSLPAGMIAGRSGATTSAADSLQIRLFGRGAHGSMPEASI
                     DPVVMAAATVLRLQTIVSREVAANEAAVLTIGVLQAGTKENVIPDEAIIKLNVRTFDE
                     GVRKRVLAAIERIVNAEAAASGAPKKPEITPLDRYSLVTNDPDATRRVRDAFRLHFPA
                     DRVQESQPTTASEDFGSFGSEWGAPSVFWFIGGIDSDMYAKAKKEGRIGEIPTNHNPR
                     FAPVIHPTLQTGVEALVVAAQAWLSA"
BASE COUNT          344 a          539 c          497 g          300 t
ORIGIN      
        1 aatcgggcga agcgcgccac gctcacgcgg gtgagcggtt cggccccgtt ttatgctggg
       61 cgcgcgatcc actcggactg cttgagctat tgtccgtctt actgacggat ccctcgtgca
      121 gacggttcgt cggacgggcg cgcgattgga agttgccagc cgtgccgtat tgccataaat
      181 ctgagcgcaa agcacagcac tgcgccggct gtagcagcga ccgacgatga aagatgcagc
      241 atgctcccga ttacgacccc cgccgcgccg atgagggccg caaccgcgta gagctccgtg
      301 cgtaacacgg tggggatctc tctcacgagc acatcgcgaa ccatgccgcc tccgattcca
      361 gtcaacatgc cgagcagcgt cgccgccacc ggccccgcgt gaaacgccag cgccttgccc
      421 gcgccggaaa ccgcaaaaag cgccagtcca gcggcgtcga acaccagcac ggggctgctg
      481 agccggttga tgattcgata ccaataaaac gtgatcagcc cggcaagaat cgagacaccg
      541 acatatcgcc agtcgctgat cgctgctgga ggaatggcgc cgatcatcac gtcgcgggcg
      601 atcccacccg agttgcccgc cgcgaacgac agaaccagaa ccccgaacag atcaagccgg
      661 tgcttcactc cagccgtcgc gccgctaagt gcgaacacga acgtgccacc aagatcgaag
      721 acaaccagaa gcgtgttgag caggaggtgt ccgttgatga ccatctcgcc ttatgctgag
      781 agccaggcct gggcggcaac taccagagct tccaccccgg tctgcagagt tggatgaatc
      841 accggtgcga agcgggggtt gtgattggtc gggatctcgc cgatccggcc ctcttttttg
      901 gccttcgcgt acatgtcgga atcgattccg cctatgaacc agaagacgga cggcgcgccc
      961 cattcggatc cgaacgatcc gaagtcctcg ctcgccgtgg tcggctggct ttcctggacg
     1021 cgatcggcag gaaagtgcag acgaaacgcg tcgcgcactc gcctggtcgc gtccggatca
     1081 ttcgtcacca gagaataacg atcgagaggc gtgatctccg gtttcttcgg ggcgcctgag
     1141 gcggcggctt cggcgttgac aatccgctcg atggcagcga gcacgcgctt acgaacaccc
     1201 tcgtcaaaag tgcgcacgtt cagcttgatg atggcctcat cagggatcac attttccttc
     1261 gtaccagcct gcagcacacc gatggtgaga accgctgctt cattcgccgc cacctcccgg
     1321 gaaacaatgg tttgcagccg cagcacggtt gcagccgcca tcaccaccgg atcgatgctg
     1381 gcctccggca ttgaaccgtg agcgccgcgt ccgaataatc gaatctgaag actgtcggcg
     1441 gcggacgttg tcgctccgga acgaccggcg atcatgcctg caggcaagct catcacatgc
     1501 tggccgagga tgacgtcggg tttggggaag cgccgaaaga gcccatcatc gatcattgcc
     1561 tgcgcgcccg cggctgtctc ttcggccggt tgaaaaacgg gcatcagcgt ccctttccat
     1621 ttgtcacgag ccttcgcaaa cagcgtggcc gcgccgatca gccaggtcac atgcatatcg
//
LOCUS       DATIMH010001005         6128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1398138, whole genome shotgun sequence.
ACCESSION   DATIMH010001005 DATIMH010000000
VERSION     DATIMH010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398138"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VLI90_17520"
     CDS             complement(<1..470)
                     /locus_tag="VLI90_17520"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HSV16065.1"
                     /translation="MTALNPSGDFGEEPITPQSSNYEMGDSGEAVRQLNSLLRGEISA
                     AETYRNVLDKVRDGDHAQDAGLLRQIQEEHGRAAQSLRDRIRELGGEASDNSGAWGVW
                     AQAVQGTMSLFGGDMGALKALKEGEEHGLKDYEEAIDDVDASSAQLMQNQLIPA"
     gene            complement(608..901)
                     /locus_tag="VLI90_17525"
     CDS             complement(608..901)
                     /locus_tag="VLI90_17525"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSV16066.1"
                     /translation="MALTKSEIAAQLADKVGISKKQVNQFFEAQAELAYKQAKNSFQI
                     PGIGKLVLAKSKPREMVMQFGEKKGQTIKVPAKTRVKFRIAKAAKDAILGAKK"
     gene            complement(1270..1458)
                     /locus_tag="VLI90_17530"
     CDS             complement(1270..1458)
                     /locus_tag="VLI90_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16067.1"
                     /translation="MRHAIRGCLNAMRLSAFALEASMTPDDAAEFLGYLENSADKLLA
                     LIEQWDALPEIEAASITR"
     gene            complement(1566..2507)
                     /locus_tag="VLI90_17535"
     CDS             complement(1566..2507)
                     /locus_tag="VLI90_17535"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSV16068.1"
                     /translation="MSAYATVRVDLARIRRNAEEISKRTGVELIAVVKADAYGLGADI
                     VAATIGDIVDAFYVFDVAEVAAGRLRETAERRIIALMGKSDNPDDYLALDVHPVVWTV
                     ERAKALKNARPVLSVDTGQQRFGCAIQDVERIRKAGECDEIMTHATDMSQVKTFLDAV
                     VGGDAKNRERLGLRLHAAGSELLEEPQAWLDAVRPGLALYNEAAQVTARLVEVRDSHG
                     PAGYSGFNVPRLGLILAGYAHGLRPGPCRVNGAPRRVLEVGMQSSFIEIGKGDRVGDE
                     VVLLGDGVSLEAIAEQWHTRPHEALLQLAGLGPKTYK"
     gene            2881..4047
                     /gene="xylA"
                     /locus_tag="VLI90_17540"
     CDS             2881..4047
                     /gene="xylA"
                     /locus_tag="VLI90_17540"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HSV16069.1"
                     /translation="MPATAAAKQYKFTFGLWTVGNPGRDPFGMPTRDVISPPQIADLL
                     GEVGAYGVNFHDNDLIPIDATPNEAEQIKKDFKRALKANGLVVPMATTNLFGDPAFKD
                     GAFTSNNAAVRAYAIQKTMRAMDLGAEFGAKIYVFWGGREGVETDAAKDPVEAIKRFR
                     EAINFLCEYSIDRKYNYKFAFEAKPNEPRGHIYFAVTGSYLALIPTLDHPEMCGVNPE
                     VAHEHMAGLNFVHQVAAALEQGKLFHIDLNDQEFGRYDQDFRFGSVNLKHAFFLVKLL
                     QDRGYNGPRHFDSHAYRQSDLQDVKDFARGSMRTYELLAERAQRWNNDKEIQALLQEI
                     NVQDAGLSKLTKKFNAANAKKLLAAPLDRVELAKARLPYERLDQLTMEVIMGAR"
     gene            4086..4493
                     /locus_tag="VLI90_17545"
     CDS             4086..4493
                     /locus_tag="VLI90_17545"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSV16070.1"
                     /translation="MDRDCGVASPRSIYPMPPVADYETNDLLVRQVEGVTIVRVRYPN
                     LDGLLEIQRITAELDALLARGVRKLVFDFKYVTHAGSAALGLLIALQRKIDARGGTLV
                     LSHAERVEELLTVSRTASLFKRAPDPRAAFKMF"
     gene            4717..5955
                     /locus_tag="VLI90_17550"
     CDS             4717..5955
                     /locus_tag="VLI90_17550"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16071.1"
                     /translation="MRKKLCSFIAGFSLLASSALAAPHEARVPLHDGRLRLADLSAQL
                     LEEAHLPTTHLPPGEISLRGIGGSLFVEAMNRSLGDGCRMNVSADALVLHVDPDKLPA
                     SMDTAERALRIFTATAAPEATAAQARHYGLLMPSAVDPNKPMVVLVHGLDMDGMEWGP
                     LGQLLKRSGYQVAYFGYPDDQPLDDDGQLLAKHLQAVRETFPAMRLNVITYSMGSLVA
                     RSYIEGPDYRGGVDHLILLAPPNHGSSWARLRILAEWKEHLLLCLNDRDWRPTWMITD
                     GLGEAGRDLNPHSNFLRELNARPRRDDVRYTIIEGDEHPMRRLTADFVQGSEMLIPGR
                     AMNWWGLRQTRVGLSLLATKIRSHADQSDGPVTLTSAALPGVADVVQVHADHETIYRG
                     DGDQLPAAWQIIKDRLETKP"
BASE COUNT         1118 a         2033 c         1825 g         1152 t
ORIGIN      
        1 tgcgccggga tcagctggtt ctgcatcagc tgggcactgc ttgcgtccac gtcgtcgatt
       61 gcttcctcgt aatccttcag cccgtgctct tcgccttctt tgagggcctt gagcgcgccc
      121 atgtcgccgc cgaagaggct catcgtgcct tgcaccgcct gggcccagac gccccaggcg
      181 ccggagttat ccgatgcttc cccgcccagc tcgcgaatgc gatcgcgcag gctctgtgcg
      241 gcccggccgt gttcttcctg gatttgccgc agcagccccg cgtcctgcgc gtggtcgccg
      301 tcgcgcacct tgtccaggac attgcgatat gtttcggccg cggaaatctc gccgcgaagc
      361 agcgaattca gttgccgcac cgcttcaccg ctgtcgccca tctcatagtt gctcgattgc
      421 ggggtgatcg gttcctcgcc gaaatcgccg ctcggattca atgcagtcat ggtggctccc
      481 ttcgttaaat tacctaagga ggggagcaac ctctgtgcct gcccgccaca gctcgtcgag
      541 ccaaaaaaga aaccgcggac ccaacgggcc cgcggttgcc tttgattcat cagtgctgaa
      601 tctcggatta cttcttagcg ccgaggatcg cgtccttggc ggccttggcg atgcggaact
      661 tcacccgcgt cttggccgga accttgatgg tctggccctt cttttcgccg aactgcatga
      721 ccatttcgcg gggcttgctc ttggcgagca cgagcttgcc gatgcccggg atctggaagc
      781 tgttcttcgc ctgcttgtag gccaactcgg cctgggcttc gaagaactgg ttgacttgct
      841 tcttgctgat gccgaccttg tcggcgagtt gggcggcgat ttcggacttg gtcaaggcca
      901 tgggtggtat ctcctttcga gagatatgcg tgccgcggcg cgatcgagtc gcggcgcggc
      961 aggggagcca ttacgttcgc cggcagtgcc aaccctggca ccctccacgg cgaagcgcga
     1021 tcgtacccgt cgatcgcgat ggcgtgaaaa actacctata tttcgcgggg tttcaatccc
     1081 ccgcgcgcaa aaatcgctgg attttccgta acaatcgcgc caccagacca cttttcgccc
     1141 ggtctgaccg accgattgcg gcggtctggc ccgcgcaaac cgcgtcacct attatcgcgc
     1201 aacgtccgcg aattccttta gtttttacgg tggtgggata gagcaggcgt gctcaggaaa
     1261 cggtagtcgt cagcgggtta ttgaagcggc ttcgatctcc ggcagcgcgt cccactgctc
     1321 gatcagcgcc agcaacttat ccgcggagtt ctccagatag ccgaggaatt ccgccgcatc
     1381 gtcgggcgtc atcgacgcct ccagtgcgaa cgcgctcagt cgcatcgcat tcagacaacc
     1441 gcggatggcg tggcgcatgc gggacacatc ttcaggcggc acggacttcc tcctctccca
     1501 aaccccgcac tttactgtgc cccgcgtcgc cccgggaacg cctgaaaaac cgcacgccca
     1561 aaagatcact tgtacgtctt cggccccagc cccgctaact gcagcagcgc ctcgtgcggc
     1621 cgagtatgcc actgctcggc gatggcctcg agactcacgc catcgcccag cagcacgact
     1681 tcgtcaccca cgcgatcgcc cttaccaatc tcgatgaacg acgactgcat ccccacttcg
     1741 agcacccgcc gcggcgcgcc gttcacgcgg caaggtcccg gccgcaggcc gtgcgcgtag
     1801 ccggccagga tcagaccaag ccgcggcacg ttgaagccgc tgtaccccgc cggtccgtgg
     1861 ctgtcgcgca cttcaaccag ccgcgctgtg acctgcgcgg cttcgttata cagcgccagc
     1921 cccggccgca cggcgtccag ccaggcctgc ggttcctcca gcaattccga ccccgccgcg
     1981 tgcagccgaa ggccaagccg ctcgcggttc ttcgcgtcgc caccgacgac cgcgtcgaga
     2041 aaagttttca cctgcgacat gtctgtcgca tgcgtcatga tctcgtcgca ctcgccggcc
     2101 ttgcggatgc gctcaacgtc ttgaatcgca cagccgaatc gctgctgccc cgtgtccacc
     2161 gacaacaccg gccgggcgtt cttcagcgct ttggcgcgct ccaccgtcca cacgaccgga
     2221 tgaacatcca gcgcgagata atcgtccgga ttatccgact tccccatcag cgcgatgatc
     2281 cgccgctcgg cggtctcgcg caatcgcccc gcggccactt cggcaacgtc gaagacataa
     2341 aaggcatcaa cgatatcgcc aatggtcgcg gcaacgatat cagcgcccag cccatacgcg
     2401 tcggccttaa cgacggcgat cagctcgacg ccggtgcgct tggagatctc ctccgcgttt
     2461 cgccggatcc gcgccagatc aacccgcacc gtcgcataag cactcatcgc cgccatcgtg
     2521 cccgctattc aatccggcgg gggcgcgttc gtccagcgtc ctctgtggct ccatgcgtca
     2581 gattcgcgcc aagacgaagg aagagatttc aaattctaac gcagagacgc agagacgcag
     2641 agacgcagag acgcggagag gtgccaagac gtcgaatact tctccgcgcc tccgcgcctc
     2701 ggcggttcaa atctttttct tcccttggcg ctcttggcct cttggctcct cttggcgtct
     2761 tggcgcgaaa cttgtttcaa ctcgtcccgg actcacgaat tcttgattca ccccgcgaat
     2821 tcggtgacac tccccgcccc tgcacattgt ggaatagtcc cgaaaaggaa ggaaaaacag
     2881 atgcccgcca ccgctgccgc gaaacagtac aagttcacct ttggcctctg gacggtcggc
     2941 aaccccggcc gcgatccgtt cggcatgccg acgcgcgacg tcatctcgcc gccgcagatc
     3001 gcggacttgc tcggcgaggt cggcgcgtac ggcgtgaact tccacgacaa cgatctgatt
     3061 ccgatcgacg cgacgccgaa tgaggccgag cagatcaaga aggatttcaa gcgggcgctg
     3121 aaggccaacg gcctggtcgt gccgatggcg acgacgaatt tgttcggcga ccccgccttc
     3181 aaggatggcg ccttcaccag caacaacgcc gccgtgcgcg cttacgcgat tcaaaagacg
     3241 atgcgggcga tggacctggg cgcggaattc ggcgcgaaga tctatgtctt ctggggcggc
     3301 cgcgaaggcg tagagacgga cgcggccaag gacccggtcg aggcaatcaa gcgcttccgc
     3361 gaggcgatca acttcctgtg cgaatatagc atcgatcgta aatacaacta taagttcgcg
     3421 ttcgaggcca agcccaacga gccgcgcggg cacatttatt tcgccgtgac cggctcgtat
     3481 ctcgcgctga tccccacgct cgatcacccc gagatgtgcg gggtgaatcc ggaagtcgcc
     3541 cacgagcaca tggcgggctt gaacttcgtc caccaggtgg ccgcggcgct ggaacaaggg
     3601 aaattgttcc acatcgacct gaacgaccag gaattcggcc gatatgacca ggacttccgc
     3661 ttcggctcgg ttaacctgaa gcacgcgttc ttcctcgtga agctgctgca agaccgcggg
     3721 tataatggtc cgcggcactt cgattcgcac gcgtatcgcc aaagcgatct acaggacgtc
     3781 aaagacttcg cccgcggatc gatgcggaca tacgaactgc tggccgagcg cgcccagcgc
     3841 tggaacaacg acaaagaaat ccaggcgctg ctgcaggaga tcaacgtgca ggacgcgggg
     3901 ctgtcgaagc tgacgaaaaa gttcaacgcc gccaacgcga agaagctgct ggccgccccg
     3961 ctggaccgcg tcgagctggc caaggccagg ctgccgtacg agcggctgga ccagctgacg
     4021 atggaagtga tcatgggcgc gcgctgatct tcaactggca tccgaaaacg ccgatgtagg
     4081 agtagatgga ccgcgattgc ggtgtcgctt ctccgcgatc catctaccct atgcctcccg
     4141 tcgctgacta cgaaaccaac gatctgctcg tgcgccaggt ggaaggcgtg acgatcgtgc
     4201 gcgtgcgcta cccgaacctg gacggcctgc tcgagatcca gcgcatcacc gccgagctgg
     4261 acgcgctgct cgcccgcggt gtacgcaagc tggtcttcga cttcaaatac gtcacccatg
     4321 ccggaagcgc cgcactgggc ctgttgatcg ccctgcaaag aaaaatcgac gcccgcggcg
     4381 gcacgctggt cctgtcgcac gccgagcgcg tcgaagaact gttaaccgtc tcgcgcaccg
     4441 caagcctgtt caagcgcgcc cccgatccgc gggcggcgtt caagatgttt tagctttgcc
     4501 ccccacgccg ggttcgccag gtactccgac gacccggatc gttttcgcgc gccggaaaat
     4561 ccgggtcgtc gcgagtacgc tccggacccg gcgtggggag tgcacgcgct cacctgctgc
     4621 cggctgcggt tatacgaatc tgcagttttt caatctgcgc ttccgccacc cgcttgctac
     4681 aattatcgcg cgcttgctgt tcagggagac cgccccgtgc gaaaaaagct gtgctcgttt
     4741 attgccggtt tctcgttact tgccagcagc gcgctggctg cgccgcatga ggcgcgcgtg
     4801 ccgctgcacg atggcaggct gcggctggcg gacttgtccg cgcagctgct cgaggaagcc
     4861 cacctgccga ccacgcacct gccgccgggg gagatcagcc tccgcggcat cggtggttcg
     4921 ctgtttgtcg aggcgatgaa ccgctcgctc ggtgacggct gccggatgaa cgtcagcgct
     4981 gacgcactgg tcctgcacgt cgatcccgac aagctgccgg ccagcatgga tacggcggag
     5041 cgggcgctgc ggatcttcac cgccaccgcc gcccccgagg cgacggcggc gcaggcccgg
     5101 cattacgggc tgctcatgcc gagcgcggtc gacccgaaca agccgatggt cgtgctcgtg
     5161 cacggcctgg acatggacgg catggagtgg gggccgctcg gccagctcct gaagcgcagc
     5221 ggctatcagg tcgcgtactt cggttacccc gacgatcaac cgctcgacga cgacggtcag
     5281 ctgctggcca aacacctgca ggcggtgcgc gagacgttcc cggcgatgcg gctgaacgtc
     5341 atcacctaca gcatgggttc gctcgtcgcg cgctcgtaca tcgaaggccc ggattatcgc
     5401 ggcggagtgg accatctgat cctacttgct ccgcccaacc acggatcgag ttgggcacgg
     5461 ctgcgaattc tggcggagtg gaaggagcac ctgctgctct gcctgaacga ccgcgactgg
     5521 cggccgacgt ggatgatcac cgacggcctg ggtgaagccg gccgcgatct caatccgcat
     5581 tcgaatttcc tgcgcgagct gaacgctcgg ccgcgccgcg acgacgtgcg ctacacgatt
     5641 atcgaaggcg acgagcaccc gatgcggcgg ctcaccgccg atttcgtaca agggtcagag
     5701 atgctgatcc ccggccgggc gatgaactgg tggggcctgc ggcaaacgcg ggtgggtctg
     5761 tcgctgctgg cgacgaagat ccgcagccat gcggatcaat cggatgggcc ggtgacgcta
     5821 accagcgccg cgctacccgg cgtggcggat gtggtgcagg ttcacgcgga tcacgagacg
     5881 atctaccgcg gggatggcga tcaactcccc gcggcgtggc aaatcatcaa agatcgtttg
     5941 gagacgaagc cgtaaagcgg cgcggtcgct ttagcgatgg tggtttccac tcgcctgaat
     6001 caacgcattg aagtgcgtca gcctcttggt caagatgcgt tgttcttcag gcgcgcctga
     6061 aaagcctggt tcatcgtcaa ccggctctca agcatcaccg gcttcagttt cttcccgtcc
     6121 ccttccaa
//
LOCUS       DATIMH010001006         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2549265, whole genome shotgun sequence.
ACCESSION   DATIMH010001006 DATIMH010000000
VERSION     DATIMH010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2549265"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..122)
                     /locus_tag="VLI90_17555"
     tRNA            complement(50..122)
                     /locus_tag="VLI90_17555"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Thr,seq:tgt)
     gene            476..829
                     /locus_tag="VLI90_17560"
     CDS             476..829
                     /locus_tag="VLI90_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16072.1"
                     /translation="MTPTLAAAVALALITADDNASASPSTAPAAAMSQATPLDTMRKL
                     SAATKANDRQAIDDCLTDEGEHPATSALIRSELHMNAASYRLTRAFSAAFGDQMRLKR
                     FGFDLFRRSMAALRC"
     gene            823..1260
                     /locus_tag="VLI90_17565"
     CDS             823..1260
                     /locus_tag="VLI90_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16073.1"
                     /translation="MLIDRSLQTSNDHPTVDIQNDVANVHLDLSPDALATGGPPQQRR
                     WAGAACVFKLEKDKWKLNLDRTMNLQYRYALNDPQADKDATLLRLGNAITDGLNDVAN
                     QVENGQIDTATDASAAVEKSAQQAFTTCGVQLANSDLLPASVR"
     gene            1332..1643
                     /locus_tag="VLI90_17570"
     CDS             1332..1643
                     /locus_tag="VLI90_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16074.1"
                     /translation="MAASEVKPGQTVRVTISKSINRESARKTIERLFMKDKAVVAPLA
                     ARSKNFKALPKRRGGRIWTKRPNKIHPELNKGVAATIVATPQAIRDLNSVADFVQVAA
                     K"
BASE COUNT          418 a          632 c          607 g          361 t
ORIGIN      
        1 aacagggctg ctatcaatcg aacggccgga tcacctgaat gaaatgagaa gccaccttcc
       61 ggagtcgaac cggaaaccgc cggtttacaa aaccggtgct ctgccaattg agctaaggtg
      121 gcgcgagacg cttgcgctcg cgtcactgca taagtttggt tgcttcgggc cgacgtgtca
      181 attctgcgat tgccggccag gatctgccgg tgttgcagaa tccagaatta ctttccagat
      241 ccccccacgc cgggttcggt actccgacga cccggattct tccgacgcgc cgcagaatcc
      301 gggtcgtcgg agtacctccg aacccggcgt gagggggtgc tacatgctct tatgactttc
      361 tcgcgcgatc ggctgttacc atgcacggcg acggtccaac aaaggcacac gcatggttga
      421 tccactgcat gagttgcgcc cgaatagctc ccgcccgccc gcgcggtggc ggtggttgac
      481 gccgacactc gccgccgctg ttgcgctcgc ccttatcacg gcggacgaca atgcttcggc
      541 atcacccagc accgctcccg cggccgcgat gagtcaggcg acgccgctcg acacgatgcg
      601 caaattatcc gccgcgacca aagccaacga tcgacaggcg atagacgact gcctgactga
      661 tgagggggag cacccagcaa catcagcgct gatccgctcg gaactgcaca tgaacgcggc
      721 gtcataccgt ctgacgcgcg cattctccgc cgccttcgga gatcaaatgc gtctgaagcg
      781 tttcggcttc gaccttttcc ggcgctccat ggcggccttg agatgctgat cgatcgttcg
      841 ctgcaaacgt ccaacgatca cccaaccgtt gatattcaga acgacgtcgc caacgtccat
      901 ctcgatttgt cgccggacgc gctagccacg gggggaccac cccagcagcg ccgctgggcg
      961 ggggcagcgt gcgtcttcaa gctcgagaaa gacaagtgga agctgaacct cgatcgaacg
     1021 atgaacctgc aatatcgcta cgcgttgaac gacccgcagg cggacaagga cgcgaccctg
     1081 cttcgcctgg gaaatgcgat caccgacggg cttaacgatg tagccaatca ggtcgagaac
     1141 ggccaaatcg acaccgcgac cgacgcatcg gccgccgtgg aaaagtcggc ccagcaggcg
     1201 ttcaccacct gcggggtgca actggcgaac tctgacttgc tgccggcgag cgttcgataa
     1261 ggcgctccgc ccccttgtca cctttgcgcc ggtttgtata ctcctccgct cttttcgacg
     1321 gagaatctga tatggcagcc agcgaagtaa agcccgggca gacggttcgg gtgacgatca
     1381 gcaagagcat caaccgtgag tcggcccgca agacgatcga gcggctgttc atgaaggaca
     1441 aggccgtcgt cgccccgctg gcggcgcgta gcaagaactt caaggcgctg cccaagcgcc
     1501 gcggcgggcg gatctggacg aagcgcccga acaagattca cccggagctg aacaagggcg
     1561 ttgccgcgac gatcgtcgcg acgccgcagg cgattcggga tttgaacagc gtcgccgact
     1621 tcgtgcaggt cgcggcgaag taatcgcgcg atcgccgaat caggacaaaa cagacggcac
     1681 agcgtcgtga tgcgaggctg tgccgttttg cttgcgcggt agcagacata gatccattga
     1741 tagccgcagg cgacagccag cgggggcgct cgacaatcag ccgggctgga attcgacagc
     1801 cccgctggct gtcgcctgcg gctttgaaag atcttgcgct gcagcgccag cgaaccgctt
     1861 gtttcccacc cggcgcgatt ttagaatcac gcaacaattc gccaccgtcg gggtgacgat
     1921 cattcgccag atctgggagg actcgcatca tggccagcaa cgcggataat tggaagtggt
     1981 ggtacttcgt cgtcgccggc gttctgatcg gcgcgtac
//
LOCUS       DATIMH010001007         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4282524, whole genome shotgun sequence.
ACCESSION   DATIMH010001007 DATIMH010000000
VERSION     DATIMH010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4282524"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VLI90_17575"
     CDS             complement(<1..744)
                     /locus_tag="VLI90_17575"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV16075.1"
                     /translation="MQKQPGEHDALPAFFHFRTKRRRDSLMDMNKLYRTFLAAGFAVA
                     AMAAVGCSQVDENHKKFVKDNRGQFDVMRLQLTYEVAAEQYKTGELDKCRETLKSALA
                     VPTPYAPMHVLAGKLEMEGDHGNLETAAVELKKAVEINGEDPEPYYLLGVVYQRWQKF
                     ETACGYYQQAVDHKSSEASYVLAVAEMKMTLGQLDDAQQLLEDKMLYFEQSGAMHIAL
                     AKIATLKGDHAAAAKHLRDATLLLPEDQNV"
     gene            complement(747..2759)
                     /locus_tag="VLI90_17580"
     CDS             complement(747..2759)
                     /locus_tag="VLI90_17580"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HSV16076.1"
                     /translation="MRKMKNVVTSGAFFLCLGGWVYSGQVSGQVEGTQAREAVLNAAA
                     AEAAAPGATDAAATPADAVPETTAGPALAQAAEPGPAPGVTVPPATTQKSTDLTAPEI
                     KVDVAGNVTQVNMSGMDINTALHFLSLQTKRNIIASKNVSGTVTVNLYDVTFAQALEA
                     ILRPNGFDYIEKGNFIYVYTDKEIDEIRKRDRKTVNRIFNLKYMNAQDASVLVKPMLS
                     SAGAMALTPPGINGLPTGATDTGGENYATDALLVINDYPENLDEVTAALKKMDVRPKQ
                     VLVEATVLNATLSDNNALGVDLVSLSGLNFSDIGSVISPISGSATTDTGTGSTSATNV
                     TTPGQLGGTLIPGNSNKQLDIGTNFAKDVPTGGLSIGFLSNHISVFLRALETVTDTTV
                     VANPKILALNKHHGEVHIGAKLGYLTTTTSQTTTQQTVQFLDVGTKLVFRPFIAEDGY
                     IRMEIHPEDSAGTIDSRGVPQTQTTEVTSNVMIKDGRTIVIGGLFRESTSASKGQVPI
                     LGNIPVLGVPFRSTNDKTQRSETIVLITPHVINDDTSLYEESMKESADATRMMLGSRA
                     GLQPWGRDRIAKLWYAKAQEEMEKGDKEKALMYVDWALNTNSRFIEAIRLREQITNRK
                     MDEAIDSSIADLVKNTLRGDAATTPDKGGSGVYPPAAPAAPAAAPK"
     gene            complement(2806..>3115)
                     /locus_tag="VLI90_17585"
     CDS             complement(2806..>3115)
                     /locus_tag="VLI90_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16077.1"
                     /translation="PTTPERVDPPNVVTASPPVVDADAVKRARAAAIREQSRGLVVKS
                     TTVGNAGSQSMAIVNQQLLTVGQEISGFEITAIRAREVEFVKDGVTIAVKMADDPRGQ
                     "
BASE COUNT          516 a         1053 c          871 g          675 t
ORIGIN      
        1 cacgttctga tcttccggca aaagaagcgt agcgtcgcgg agatgcttcg ccgccgcggc
       61 atgatcgccc ttgagcgtcg caattttcgc cagcgcaatg tgcatcgccc ccgactgctc
      121 aaagtagagc attttgtctt caagcaactg ctgcgcgtca tcaagctgcc ccagcgtcat
      181 tttcatttcc gccacggcca gcacgtaaga cgcctcactc gatttatgat ccaccgcctg
      241 ctggtagtac ccgcacgccg tctcgaactt ctgccagcgc tgatagacca cgcccagcag
      301 atagtaaggt tcgggatcct ccccgttgat ctccaccgcc ttcttcagct ccaccgcagc
      361 ggtttcaagg ttcccgtggt ccccctccat ttccagcttt ccggccagca cgtgcatggg
      421 cgcgtacggc gtgggaacgg cgagcgccga tttcagcgtc tcccggcact tgtccagctc
      481 gccggtctta tactgctccg cggccacttc atacgtgagc tgcagccgca tcacgtcgaa
      541 ctgtccgcgg ttgtccttca cgaacttctt gtgattctca tcaacctgac tgcagccgac
      601 ggcggccatc gccgccacgg caaacccggc ggccaggaag gtgcgataaa gtttgttcat
      661 gtccatgaga gagtcccttc gtcttttcgt tctgaaatga aaaaatgccg ggagagcgtc
      721 atgctctccc ggctgcttct gcaaccttac ttgggagccg cggccggtgc cgccggagcc
      781 gccggcgggt acacgcccga gccgcccttg tccggcgtcg tcgccgcgtc gcccctgagc
      841 gtgttcttca cgagatccgc aatcgagctg tcgatcgcct cgtccatctt ccggttcgtg
      901 atctgctcac gcagccggat cgcctcgatg aaccgcgaat tggtgttcag cgcccaatcc
      961 acgtacatca gggccttttc cttgtcgccc ttctccattt cttcctgagc cttcgcgtac
     1021 cagagcttcg cgatgcggtc gcggccccag ggctgcaacc cggcgcggct gcccagcatc
     1081 atccgcgtgg cgtcggcgct ttccttcatc gattcttcgt acagcgacgt atcatcgttg
     1141 atcacgtgcg gcgtgatcag cacgatcgtc tccgagcgct gcgtcttgtc attggtcgaa
     1201 cggaacggca cgcccagcac cgggatattg cccaggatcg gaacctggcc cttcgacgct
     1261 gacgtcgact ctctgaacaa cccgccgatc acgatcgtcc ggccgtcctt gatcatcacg
     1321 ttgctggtca cttccgtggt ctgcgtctgc ggcacgccgc gcgaatcgat cgtgccggcg
     1381 gaatcctcgg ggtgaatttc catgcggatg tagccgtctt ccgcgatgaa gggccggaag
     1441 accagcttgg tgccgacgtc caggaactgc acggtctgct gggtggtcgt ctggctggtg
     1501 gtcgtcgtga ggtagcccag cttggcaccg atgtgcactt cgccgtgatg tttgttgaga
     1561 gcgagaatct tcgggttcgc caccaccgtg gtgtcggtga cggtttcaag cgcccgcaga
     1621 aacacgctga tgtggttcga gaggaacccg atcgaaaggc cgcccgtcgg cacgtctttc
     1681 gcgaaattcg taccgatgtc gagctgcttg ttggagttgc cggggatgag cgttccgccg
     1741 agctggcccg gcgtggtcac gttcgtggcc gaggtggacc cggtgcccgt atccgttgtc
     1801 gcgctgccgc tgatcgggct gatcaccgaa ccaatgtccg aaaaattcag gcccgagagg
     1861 ctcaccaggt caacgcccag agcattgttg tcgctgagtg tggcgttcaa cacggtcgct
     1921 tcgaccagca cctgcttggg ccgcacgtcc atctttttca gggcggcggt cacttcgtcg
     1981 aggttctcgg gataatcgtt gatgaccagg agcgcatcgg tggcgtagtt ctcgccgccc
     2041 gtgtcggtcg cacccgttgg caaaccgttg atgcccggcg gggtcagcgc catcgcgccc
     2101 gccgacgaga gcatcggctt gacgagcacc gacgcatcct gggcattcat atacttgaga
     2161 ttgaagatgc ggttgaccgt tttgcggtcg cgcttgcgga tctcatcgat ttccttgtcg
     2221 gtgtagacat aaatgaagtt gcccttctcg atgtaatcga agccgttcgg ccgcagaatc
     2281 gcctcaagcg cctgggcgaa cgtcacgtca tacagattca ccgtgaccgt gcccgagacg
     2341 ttcttcgacg caatgatgtt ccgcttggtc tgcagcgaca aaaagtgcag cgccgtgttg
     2401 atgtccatcc cggacatgtt cacctgcgtc acgttgcccg ccacatccac cttgatctcg
     2461 ggcgccgtga ggtccgtgga cttctgcgtc gtggccggcg gcaccgtcac gccgggcgcc
     2521 gggcccggtt ccgccgcctg cgccaacgcc gggcctgcag tcgtctccgg cacggcatcc
     2581 gctggcgtgg ccgccgcgtc tgtcgccccg ggggccgccg cttccgccgc cgccgcgttt
     2641 aacaccgcct cccttgcctg ggtcccctca acctgcccac ttacctgacc gctataaacc
     2701 cacccaccaa ggcataaaaa aaacgccccg gacgttacga cattcttcat cttccgcatg
     2761 aaaggctcct tttaggctag cggacaacac gacgacagtc aggctttact gcccacgcgg
     2821 atcgtcggcc atcttcaccg cgatcgtgac gccatctttg acaaactcaa cttcccgcgc
     2881 acgtatcgcg gttatttcaa atcctgatat ctcttgcccc accgtcagca actgctgatt
     2941 cacgatcgcc atcgactggc tccccgcgtt ccccaccgtc gtcgatttca ccaccagccc
     3001 gcgcgactgc tcccgtatcg ccgccgcccg cgcccgcttc accgcgtccg catccaccac
     3061 cggcggtgat gccgtcacaa cgttcggcgg atccacgcgc tcaggcgtcg tcggc
//
LOCUS       DATIMH010001008         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3988570, whole genome shotgun sequence.
ACCESSION   DATIMH010001008 DATIMH010000000
VERSION     DATIMH010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3988570"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VLI90_17590"
     CDS             complement(<1..232)
                     /locus_tag="VLI90_17590"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HSV16078.1"
                     /translation="MDPRLQHGVDLFNRREFFVCHAVLEEIWMPERGPRRRFLQAIIH
                     FAVAFYHDQRGNPLGASRQLRKGLRKLAPYLPS"
     gene            complement(235..852)
                     /gene="msrA"
                     /locus_tag="VLI90_17595"
     CDS             complement(235..852)
                     /gene="msrA"
                     /locus_tag="VLI90_17595"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSV16079.1"
                     /translation="MLPVAFLLFAASAAEFPAPAVDPPSPTHTAVLAGGCFWGIEAVF
                     EKLKGVTDVVSGYAGGQQQTAHYEMVGSGRTGHAESVQITYDPTQISYGKLLQVFFSV
                     AHDPTQLNRQGPDEGTQYRSAIFYASDEQKRVAEAYIQQLNQSKVFKRPIVTQLTPLQ
                     GFYAAEDYHQDYIAHNPGNPYVIYNDLPKLAELNKKYPDLVVKKR"
     gene            906..2495
                     /locus_tag="VLI90_17600"
     CDS             906..2495
                     /locus_tag="VLI90_17600"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV16080.1"
                     /translation="MQRRDLLRSAGAGLLILKSGTLRGQNAPSNKLNVALIGVWGRGT
                     AHYNVLKNENVVALCDVNDNRTKEALELFPNAKTHWDWRRCLEQKDVEAVIICTADHH
                     HAFIANWALNRNMHVYCEKPLGISVEETRTVRANYLSKRAKLATQHGTQRHAYPNFER
                     IRELILDGAIGDLKTVHAWDARQLPRPGYPAAEGMAPSFMHYEQWIGPSPYHPYSPQY
                     FGGTAGLNCLFWNMYRDFGVGQMGDMGAHTMDLLWNSIDAGAPTSIDIDREVSDKFDP
                     NITPVKLKAIFEHPANSWRGPIELVWYQGGLKPAPPKAYIDVTRVGNGAIFEGTRGSI
                     FADFTSRLVIPNNDDGDFTYYKRRAKSETLPLIGGTGQVTQAAARPRPAGGQQGGRGG
                     RGPAGPLPGGMRAMPSAEPGPNGFPVVQMLDNGVPPALGLPNPSLHATGNTGDSLFQL
                     DWIDACKGKNNNVVHGTSSKTHCDFDYSGTMIEQMLLGLVAHQVGKKLEYDPKTGRVT
                     NSSEANDYLKRTYRSGWTLNG"
BASE COUNT          511 a          773 c          825 g          443 t
ORIGIN      
        1 gcgacggcag gtaaggcgcg agcttccgca aacctttccg aagctgacga ctcgcgccga
       61 gcggattgcc gcgctggtcg tgataaaaag cgacggcgaa gtgaataatc gcctgtagga
      121 accggcggcg cggaccgcgc tcgggcatcc agatctcttc cagcactgcg tggcagacga
      181 agaattcgcg ccggttgaag agatcgacgc cgtgctggag ccgtgggtcc atcgtcagcg
      241 cttcttgacg acgaggtccg ggtacttctt gttgagctcg gcgagcttcg gcaggtcgtt
      301 ataaatgacg tagggattgc cggggttatg cgcgatgtag tcctggtgat agtcctcagc
      361 cgcgtagaat ccctgcaagg gcgtgagctg cgtaacgatg ggccgcttga acaccttcga
      421 ctgattgagc tgctggatgt acgcctcggc gacgcgcttc tgctcgtcgg acgcgtagaa
      481 gatcgccgac cggtattgcg tcccctcgtc gggaccctgc cggttgagct gggtcgggtc
      541 gtgcgcgacg gagaagaaca cctgcagcag tttgccgtac gagatctggg tgggatcgta
      601 ggtgatctgg acggactcgg cgtgtcccgt gcgtcccgag ccgaccattt cgtagtgcgc
      661 cgtctgctgc tggccgcccg cgtacccgga aaccacgtcg gtaacgccct tcaatttttc
      721 gaacaccgcc tcgatgcccc agaagcagcc gcctgcgaga acggcagtgt gggtcgggga
      781 tggcggatcg acggccgggg cagggaattc ggcagcggag gcagcgaaca acaggaaagc
      841 tacaggcagc atatctacga tggtaccgct tgcttcggac cttgccacgt gttacgggtt
      901 tccccatgca gagacgcgac cttcttcgct ccgccggcgc aggcttgctc atcctgaaga
      961 gcggcacttt gcgcgggcaa aatgccccca gcaacaagct gaatgtggcc ctcatcggcg
     1021 tatggggccg cgggacggcg cactacaacg tcctgaagaa cgagaacgtg gtcgccctgt
     1081 gcgacgtcaa cgacaatcgc acgaaggaag cgctcgagct gttcccgaat gccaagacgc
     1141 actgggactg gcggcgctgc ctggagcaga aagacgtcga ggcggtgatc atctgcacgg
     1201 ccgaccatca tcatgccttc atcgccaatt gggcgctcaa tcgcaacatg catgtgtact
     1261 gcgaaaagcc gctcggaatc agcgtcgaag agacccgcac cgtccgcgcc aattacctga
     1321 gcaagcgcgc gaaactcgcg acccaacacg ggacgcagcg gcacgcctat ccgaacttcg
     1381 agcggatccg ggaattgatt ctcgatggcg cgatcggcga tctcaagacg gtgcacgcgt
     1441 gggatgcgcg gcagttgccg cgtccggggt atccggccgc cgagggaatg gcgccgtcgt
     1501 tcatgcatta cgaacaatgg atcgggccgt cgccctacca cccctacagc ccgcaatatt
     1561 tcggcggcac cgcgggactg aactgcctgt tctggaatat gtatcgcgat ttcggcgtcg
     1621 ggcagatggg cgacatgggc gcgcacacga tggacctgct gtggaactcg atcgacgcgg
     1681 gcgcgccgac ctcgatcgac atcgatcgcg aggtcagcga caaattcgac ccgaacatca
     1741 cgccggtcaa attgaaggcg atcttcgagc acccggcgaa ttcgtggcgc gggccgatcg
     1801 agctcgtgtg gtaccagggc gggctgaagc cggccccacc gaaggcgtac atcgacgtca
     1861 cccgcgtcgg gaacggcgca attttcgaag gcaccagagg gtcgatcttt gccgacttca
     1921 ccagccgcct cgtcattccg aacaacgatg atggcgactt cacctattac aagcgccgcg
     1981 ccaagagcga gacgctgccg ctgatcggcg ggacgggaca ggtgacgcaa gccgccgcac
     2041 gtccgcgtcc ggcgggtggc cagcagggtg gtcgcggcgg acgcggtccg gccggtccgc
     2101 tgccgggcgg gatgcgcgca atgccgagcg ccgaaccggg tccgaatggc ttcccggtcg
     2161 tgcaaatgct ggacaatggt gtgccgccgg cgcttggtct gccgaacccg agccttcacg
     2221 cgaccggcaa caccggcgac agcctgttcc aactcgactg gatcgacgcc tgcaaaggga
     2281 agaacaacaa cgtcgtccac ggcaccagtt ccaagacgca ctgcgacttc gattacagcg
     2341 gcacgatgat cgagcaaatg ctgttagggc tggtagcgca ccaggtcggg aaaaagcttg
     2401 agtacgatcc gaagactggc cgcgttacga actcgtcgga agcgaacgat tacctgaaaa
     2461 ggacgtaccg ctccggctgg acgttgaacg gctaggagcc ggttttcttc gattcgattt
     2521 tccgcacggc tcccgtggtc ccgtaagacg cg
//
LOCUS       DATIMH010001009         3847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4282958, whole genome shotgun sequence.
ACCESSION   DATIMH010001009 DATIMH010000000
VERSION     DATIMH010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4282958"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..3651
                     /locus_tag="VLI90_17605"
     CDS             52..3651
                     /locus_tag="VLI90_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16081.1"
                     /translation="MGKARLVLLALILLASSSSGAADAPWQQLGGGSAFGNWPGLKVA
                     ADGTASLAVPGEAVFRYPDGPRGFYKHGFRVFNDGTADWHADWGVQLEVKLADARAVE
                     LMVSIAPASRGNEVESRKATVRIAGDGWHTVNLPWSAFQFEQADFAFLRFIKQLSIAA
                     AATDANQAPFNISLRNVRVVKAPSVAMESDVLGKAAPKNQAVEYPIVVSNCTDQPQAV
                     ALSLVRYGWETMDSVIEPAAVQLAPWASQACTLRVSIGDRVPPGGHEQQVVQAIANGD
                     SAASSRLTFITTSELPHPYIMHTPARWDAVREKVKRYAWAKAAQDAYVEQAEKWRVPE
                     VAQPPGNDPDDTMGPFLFRTQNENDLLACAYSWQLSGNRAHAEKVAEFLRRLSNPQNG
                     YPVTLRGCNQSLVQEGHFFQHIAMAYDMIHDAGVLSPADRAQIESTFGIFIETIERES
                     ERGAINNWNLSEDCGAFYCALAMQDLSLADRFFSGPAGIKDQLAKGTMDDGWWYECSI
                     SYNMWCASEFTQAALAYEPFGVNFKDMHVPASYSPKVMLTSRLSGGNMPDVADPDQRR
                     KPFGMNPDVFGPTRRPYRTITDLWNGLLPFVDYRGVMFGVNDSTESQVAGNRTEVSGQ
                     PFEIAYSVYRDPKYAAMIKMGGGGKRDLLYAVPDLPEQTPELFRDNASADNVGLVMLR
                     SQTPNRPMRDQIQAALHYGTHGWAHGHFDRTNLIALMRNGRNFWNPESVFWVYEPFMY
                     KFYCQTSLNQNMVVVDEKMQEAAPGERLMLYSGRAMQATAVQTTARWSNPPHGGMVYD
                     YVPVKSFAEKCWREGRSVPIPENPPAYGTLTGFTETIHQRRLMIVTDDYVVLADDIAG
                     DQPHTFESLLQLKGFLGIEAPQKQFLRHDAQWNADPVGSAQFVTDCNWYSAQAPAVAR
                     FEERWGPGADNEGSRSAGNEDGVMKLDVHSLWPPAQQIMVATAPEQHDTEKRLFYSVR
                     GDGRTLAGGKFGAWILGRGDIDVPVDGIKQLELETKTELSRHPTLFWAAAGIVTRDGK
                     EIPLSQLPMQADNIVPTKGPDQDYLGGPVKIIGEQYKQTIAAEPKDAKQPGVIRVDLT
                     GTDAVRFKAVIGSDYPPGDESQRRKVYAIRPADARAATARFITIIEPYEDRPTIKSAT
                     ATSGDHLRVELTDGRVQEIALHNLAASESGPSADLTETRDGQVLRRESTTSGN"
BASE COUNT          742 a         1198 c         1255 g          652 t
ORIGIN      
        1 ttgtgcgcac aggccggagg cccgtgtcac ggatgaggtg tgagatcttc aatgggtaaa
       61 gcacgacttg tccttctcgc gttgatcctc ctcgcgtcgt cgtcgtcggg tgctgccgat
      121 gcgccttggc aacaattggg cggaggaagc gcattcggca actggcccgg ccttaaagtc
      181 gccgcggacg gcacggcatc actggccgtg ccgggcgaag ccgtcttccg ctatcccgac
      241 ggcccgcgcg ggttttacaa gcatggcttt cgcgtcttca acgacggcac cgccgactgg
      301 cacgcggatt ggggcgtgca gctggaggtg aagctggcgg atgcgcgcgc cgtcgaattg
      361 atggtatcga tcgccccagc gtcgcgcggc aatgaagtcg aatcgcgcaa ggcgaccgtg
      421 cgcatcgccg gcgacggctg gcacaccgtc aaccttccct ggtccgcgtt tcaattcgaa
      481 caggcggact tcgcgttcct gcggttcatt aagcaactgt cgatcgccgc ggcggcgacg
      541 gacgccaacc aagcaccatt caatatctcg cttcggaacg tgcgtgtggt caaagcgcca
      601 tcggtcgcga tggaaagcga tgtgctcggc aaagcggcgc cgaagaacca ggcggtggaa
      661 tatccgatcg tcgtcagcaa ctgcaccgac caaccgcagg cggtcgcgct gtcgctggtg
      721 cgatatgggt gggagacgat ggacagcgtg atcgagccgg cggcagttca gctggcgccg
      781 tgggcgagcc aggcctgcac gctgcgcgtg agcattggcg accgcgtgcc accgggcggg
      841 cacgagcagc aggtcgtgca ggcgatcgcc aacggcgatt ccgcggcgtc ttcgcggctg
      901 acgttcatca ccaccagcga actgccgcat ccatacatca tgcacacgcc ggcgcgctgg
      961 gatgctgttc gcgagaaggt gaagcgatat gcgtgggcga aggcggcgca ggacgcgtat
     1021 gttgagcagg cggaaaagtg gcgcgtgccg gaggtcgccc agccgccggg gaatgatccg
     1081 gatgacacca tgggcccgtt cctgttccgc acgcagaacg agaacgacct gctggcgtgc
     1141 gcgtattcgt ggcagctgag cggcaaccga gcgcacgcgg agaaggtggc cgaattcctt
     1201 cgccggctgt cgaatccgca gaacggttac cccgttacgc tccgcggttg caatcagtcg
     1261 ctggtgcagg aaggtcactt cttccagcac atcgcgatgg cgtacgacat gatccacgac
     1321 gccggggtgc tctcgccggc ggatcgcgcg cagatcgaat ccaccttcgg catcttcatc
     1381 gaaacgatcg agcgcgaaag cgagcgcggc gcgatcaaca actggaacct gtccgaagac
     1441 tgtggcgcgt tctactgcgc gctggcgatg caggaccttt cgttggcgga tcgtttcttt
     1501 tccggtccgg cgggcatcaa ggatcagctg gccaagggca ccatggatga tggttggtgg
     1561 tatgaatgtt cgatcagtta caacatgtgg tgcgccagcg aatttaccca ggcggcgctg
     1621 gcgtatgagc cgttcggtgt gaacttcaag gacatgcacg tgccggcgag ttattcgccg
     1681 aaggtgatgc tcacgtcgcg gctgagcggc gggaacatgc cggacgttgc cgatcccgac
     1741 caacgccgca aaccgttcgg catgaacccc gacgtcttcg ggccgacgcg ccggccctat
     1801 cgcaccatca ccgatttatg gaacggcctg ctgccgttcg tcgattatcg cggcgtgatg
     1861 ttcggcgtga acgattcgac cgaatcgcaa gtggccggca atcgcacgga ggtcagcggt
     1921 cagccgttcg aaatcgcgta ttccgtttat cgcgatccga aatacgcggc gatgatcaag
     1981 atgggcggcg gcgggaagcg cgatttgttg tacgccgtgc cggatctgcc ggagcagacg
     2041 ccggagttgt ttcgcgacaa cgcgtcggcc gacaacgtcg ggctggtgat gctgcgctcg
     2101 cagacgccga accgtccgat gcgcgatcag atccaggcgg cgctgcacta cggcacgcac
     2161 ggatgggccc acggccattt cgatcgcacc aatctaatcg cgctaatgcg caacggccga
     2221 aacttctgga accccgagtc ggtcttctgg gtgtacgagc cgttcatgta caagttttat
     2281 tgccagacat cgctgaacca gaacatggtg gtggtggacg agaagatgca ggaagccgcg
     2341 ccgggcgagc ggttgatgct gtacagcggc cgggcgatgc aggcgacggc ggtgcagaca
     2401 accgcgcgat ggtcgaaccc gccgcatggc gggatggttt acgactacgt gccggtgaag
     2461 agcttcgccg agaagtgctg gcgcgaaggc aggtctgtgc caatccccga aaatccgcca
     2521 gcatatggca cgctcaccgg cttcacggaa acgatccacc agcgccggct gatgatcgtc
     2581 accgatgatt acgtcgtgct ggccgacgac atcgccggcg atcagccgca cacgtttgag
     2641 agcctgctgc agctcaaggg cttcttgggt attgaagcgc cacagaagca attccttcgg
     2701 cacgatgcgc agtggaacgc cgacccggtc ggcagcgcgc agttcgtcac cgattgcaat
     2761 tggtacagcg cgcaggcgcc cgcggtcgcg cgattcgaag aacgctgggg acccggcgcc
     2821 gacaacgaag gctcgcgatc ggccggcaat gaagacggcg tgatgaagct ggatgtgcat
     2881 tcgctgtggc cgccggctca gcagatcatg gtcgccaccg cgcccgaaca gcacgacacg
     2941 gaaaagcggc tgttctacag cgtgcgcggc gacggtcgca ccctcgccgg tggaaagttc
     3001 ggcgcgtgga tcctcggccg cggggacatc gatgtgcccg tcgatgggat caagcaactg
     3061 gagctggaaa cgaaaacgga attgtcccgc caccccacgc ttttctgggc ggcggccgga
     3121 atcgtcacgc gtgacgggaa ggagatcccg ctgtcgcagc tgccgatgca ggccgacaac
     3181 atcgtcccca ccaaaggccc cgatcaggac tacctcggcg gcccggtgaa gatcatcggc
     3241 gagcagtaca agcagacgat cgccgccgaa ccgaaggatg cgaagcagcc cggcgtcatc
     3301 cgcgtcgatc ttaccggcac cgatgccgtg cgattcaaag ccgtcatcgg cagcgattat
     3361 ccgcccggcg atgaatcgca gcgccgcaag gtctatgcga tccgccctgc tgacgcccgc
     3421 gcggccaccg ctcgcttcat caccatcatc gaaccgtacg aggaccgccc caccatcaag
     3481 tccgccaccg ccacgagcgg cgatcacctg cgggttgagc tgaccgatgg ccgcgtgcag
     3541 gagatcgcgc tgcacaacct cgccgccagt gagtcaggcc cctccgccga cctgaccgaa
     3601 acccgcgacg gtcaagtcct gcggcgggaa agcacgacca gcggcaactg agccctcaac
     3661 ggaggccgcg gcggctactg atggctggga tcttaaaccg cgctccctgg tctggtcccc
     3721 tctcccacgt acccgctgac gaagcgacac tttcgtacaa gcggccttga cccaatccac
     3781 gctgggttcg gaggtactcc gacgacccgg attcttgggc gcgcgcgaaa ggcatccggg
     3841 tcgtcgg
//
LOCUS       DATIMH010001010         3122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5443303, whole genome shotgun sequence.
ACCESSION   DATIMH010001010 DATIMH010000000
VERSION     DATIMH010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5443303"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1450)
                     /locus_tag="VLI90_17610"
     CDS             complement(209..1450)
                     /locus_tag="VLI90_17610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HSV16082.1"
                     /translation="MQKRSVVITGIGVVSPNGIGRDAFARACIDGRSGITWLKIDIEG
                     LRSSVAAQALDFDPLKVLDVGEARRVPRMIPMALQASREAMQQAGVDFAPTDIDAQRK
                     MGVALGTGGGGLAFVEEQYRTFFTEGTGSLYSITAGTHGNLSSELSIALHLRGPSHVL
                     STGCTSSTDAIGYARMLIQQGVVPMMLAGGADSPISKGILTAFEKLRVISTRKWDDPA
                     KASRPFTPERDGFVLGEGAWMFVLEDAEHARARGAIALAEVAGYSSTCDAYHRVQIAP
                     DVIEPVRAIELALADAGVSKEQVDYINLHGTSTQLNDKMETAAIHRCFGERAARIPGS
                     STKSMIGHPQGACGAAGLAATVLSMHAGWIHPTINLDRADADCDLDYVPNVARKQAVN
                     VALCNCIAFGSKNSAIVVKRC"
     gene            1638..2702
                     /locus_tag="VLI90_17615"
     CDS             1638..2702
                     /locus_tag="VLI90_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16083.1"
                     /translation="MRSLASVSVLKISAILAGVALASAVRAAGFTPPADGKLTEKQVT
                     TYIAVLKEQMDSLRAAGKAADGSKSGAANLAIMMRMNEKVDASIAKHGMTRDEYNWVG
                     DQVGKLWYPAVLQQQWDESGKPDLEKQIKDKEADRDVAKQKIARYEQAQKDGKRILTK
                     EERDSAVQSASGDRDSIAQEVKDRENDLKSAKEEVATHEKEAKDADDLAKNPPADVSA
                     DDRQGYIDGKKGEAQAARDAAKESRSKVADAQKALDEAKARLAVAEGKLKNPEVPVTD
                     DEKAQVKQANEQAIDDAKRTVDDDEQAIASLKDVLAAGPPKFDGGNDKTDPDNLALVK
                     KHVKEYAEAIGASDVLKTNK"
     gene            2730..>3122
                     /locus_tag="VLI90_17620"
     CDS             2730..>3122
                     /locus_tag="VLI90_17620"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HSV16084.1"
                     /translation="MTDPRIRKLADLLINYSCALKPGEKVLVEAIDVPHAFTRALVER
                     AAAAGGQALVLLKSNEINRALMLNGSDASWNTAADVERLQMESVQCYIGARGSANVSE
                     LSDVPADKQKIYESTVWKRVHHDVRIKKT"
BASE COUNT          630 a          905 c         1077 g          510 t
ORIGIN      
        1 ctccgacgtt gggctcgatg gtcgcaaacg gataattcgc cgcgagcgca cccgcggcgg
       61 tcatcgcgtt gaagatggtg ctcttgccaa cgttgggcag cccgacgatt ccaacttcca
      121 aagccatagg gcgggagagt ataggtgtcg cttgcggctt cgcaaaccac gccgcttcat
      181 ttgcgaagcc gcaagcggcg gcccggcgtc agcagcgttt gaccacaatc gcgctattct
      241 tgctgccgaa ggcgatgcag ttgcagaggg cgacgttcac cgcctgcttg cgggcgacgt
      301 tcggaacgta atccagatcg cagtcggcat ccgctcgatc caagttgatc gtcgggtgga
      361 tccatccggc gtgcatcgac aggacggttg ctgccaaccc agcggcgccg caggcgcctt
      421 ggggatggcc gatcatcgac ttcgtggaac tgccggggat gcgagcggcg cgctcgccga
      481 aacatcggtg gatggcggcg gtttccatct tgtcattcag ttgcgtgctg gtgccgtgca
      541 ggttgatgta atcgacttgc tccttcgaca cgccggcgtc agcgagcgcc aattcgatcg
      601 cccgcacggg ctcgattaca tcgggcgcga tttggacgcg gtgataggca tcacacgttg
      661 agctatatcc ggcgacttcc gccagcgcta ttgcgccgcg cgcccgggcg tgctccgcgt
      721 cttccagcac gaacatccac gccccttcgc cgagcacgaa gccgtcgcgc tcgggcgtga
      781 acggccgtga cgccttggcg gggtcatccc attttcgcgt ggagatcacg cgcagctttt
      841 cgaaggcggt caggatgcct ttgctgatcg gcgaatccgc gccgccggcg agcatcatcg
      901 gcacgacgcc ctgctggatc agcatgcgag cataaccgat ggcgtcggtg ctgctggtgc
      961 aaccggtcga aagcacgtgc gacggcccgc gcaggtgcag ggcgatcgac agttcgctgg
     1021 aaaggttccc gtgcgttccg gcggtgatgg agtagagcga gccggtgcct tcggtgaaga
     1081 acgtgcggta ttgttcttcg acgaacgcca atcccccgcc gccggtgccg agggcgacac
     1141 ccattttacg ttgcgcgtcg atgtcggttg gcgcgaagtc aacgccggct tgttgcatcg
     1201 cctcgcgcga tgcctgcagc gccatcggga tcatccgggg cactcgccgc gcttcgccga
     1261 catcgagcac cttcagcgga tcgaagtcca gcgcctgggc ggcgaccgac gatcgtagtc
     1321 cctcgatgtc gatcttcagc caggtgatgc cgctgcggcc gtcgatgcac gcgcgggcga
     1381 aggcgtcgcg gccgatcccg ttgggactga ccacgccgat gccggtgatg acgacgctgc
     1441 gtttctgcaa gacgagttgg ctccccaccg caagtacaac ccatcgtagg cggaagcgga
     1501 ttccaaggct atgcgtgtat tgcggcgatt gttacacttt ccgcgcatcg cggcgctcgt
     1561 ggtggaccga aacgcggttc ggttcgccgc atcctgagcc cgcgcgggcc gcgttcgaaa
     1621 tttggagtga gacgaacatg cgatccttag catcggtgtc ggttctgaag atttcggcca
     1681 ttcttgccgg cgttgcgctg gcatccgccg tgcgcgcggc gggtttcaca cccccggccg
     1741 acgggaagct gacggaaaag caggtgacga cgtacatcgc cgtcctgaag gagcagatgg
     1801 attcgctgcg cgccgccggg aaggcggccg acggtagcaa gtccggcgcg gcgaacctgg
     1861 cgatcatgat gcgcatgaac gagaaggtgg acgcgtccat cgccaagcat ggcatgacgc
     1921 gcgatgaata caactgggtg ggtgaccagg tgggcaagct gtggtacccc gcggtgctgc
     1981 agcagcagtg ggacgagagc ggcaagcccg acctggagaa gcagatcaag gacaaggaag
     2041 ccgatcgcga cgtggccaag cagaagatcg cgagatacga acaggcgcag aaggacggca
     2101 agcgcatcct gacgaaggaa gagcgcgact cggcggtgca gtcggcgagc ggcgatcgtg
     2161 attccattgc gcaggaagtg aaggatcgtg agaacgatct gaagtcggct aaggaagagg
     2221 tggcgacgca cgagaaggaa gcgaaggacg cggacgacct ggcgaagaat ccaccggcgg
     2281 acgtgagcgc ggatgatcgg cagggataca tcgacggcaa gaagggcgaa gcccaggcgg
     2341 cgcgcgatgc ggcgaaggaa tcgcgatcga aggtagccga cgcccaaaag gcgctggacg
     2401 aagccaaggc ccgtctggcg gtggctgagg gcaagctcaa gaacccggaa gtgccggtga
     2461 cggacgatga aaaggcgcag gtgaaacagg cgaacgagca ggcgatcgac gacgccaagc
     2521 gcacggtgga cgacgacgag caggcgatcg cgagcctgaa ggacgtgctg gcggcgggtc
     2581 cgccgaagtt cgatggcggc aacgataaga cggacccgga caacctggcg ctggtgaaga
     2641 agcacgtgaa ggaatatgcc gaagcgatcg gcgccagcga cgtgttgaag actaacaagt
     2701 aactctctga ccgatggacg gccctgctga tgactgatcc ccgcattcga aaactggccg
     2761 atctgctgat caattattca tgcgcgctaa agcccggcga gaaggtgctg gtcgaggcga
     2821 tcgacgtgcc gcacgcgttt accagggcgc tggtggaacg cgccgccgcc gccggggggc
     2881 aagcgctggt gctgctgaag agcaacgaaa tcaatcgcgc gctgatgctg aatggttccg
     2941 acgcgtcatg gaacacggcg gcggatgtcg agcggttgca gatggagagc gtgcagtgct
     3001 acatcggcgc ccgcgggagc gccaacgtgt cggaactgtc cgatgtcccg gccgacaagc
     3061 aaaagatcta cgagagcacc gtttggaagc gcgtgcacca cgacgtgcgg atcaagaaga
     3121 cg
//
LOCUS       DATIMH010001011         1973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1688533, whole genome shotgun sequence.
ACCESSION   DATIMH010001011 DATIMH010000000
VERSION     DATIMH010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688533"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..>1973
                     /locus_tag="VLI90_17625"
     CDS             159..>1973
                     /locus_tag="VLI90_17625"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSV16085.1"
                     /translation="MRHLLVLIVTIGSVVPLVATAAEAPASAPVGLSIDLSHPIPPPI
                     TGQLKMGGKNPAGVEINANSRYLTLGGKPWVPVMGEFHFTRYPHEEWERELLKMKAGG
                     ITLVSTYVFWIHHEEVQGQFDWSGDRDLRAFLQLCAKHGLLAWVRLGPWCHGEVRNGG
                     LPDWVLAECGKNIRKNDPKFMGFTRTLYQQIFKQCDGLLWKDGGPIIGVQLENEMSNN
                     PAYLLALKRLAQNVGFDVPLYSVTGWGSVKFPADEVIPLFGGYADGFWEGGSTYSKKD
                     RKQFFFTHVRDDSGIGADLMPATWHAAAAVELDRYPYVTCECGGGMETSYARRPLMSA
                     GDVAALSFVKIGSGSNLPGYYMYHGGRNPAGKLSTLQESQATNYPNDMPVIRYDFQAP
                     LGEFGQVRQSYDALRTIHVFLADFGEQLATMPAILPAEQPKSVDDTATLRWSARSDGH
                     RGFVFINNYQRGEPLPERRDVQLTLKLADGDLTFPQTPANVPSGAYTIWPFKLDLNGT
                     NLRYATAQLLCRIDDGGTPMFVFFAPAGTAAEFSIDGVGVIHPNPRNDVATTVHAAEG
                     RTARVLLLTHEQAMHAYVGDIQGARHLVLSPDGNAIFDG"
BASE COUNT          376 a          615 c          635 g          347 t
ORIGIN      
        1 agcggcgcgc agggcggcgc gacgaacgca gagcgtttcc acgcgaccgt tcaaccgggt
       61 gtgacgatgg cctactgatc gcactcgcgc cgagtgaatg gttcaacaac aggtgtaaca
      121 ctctttcgct gctgcctctt cacgtaggct accgacccat gcggcacctg ctcgtcctga
      181 ttgtcacgat tggcagcgtc gtaccgctcg ttgccactgc cgcggaagcg ccggcttccg
      241 cgccggtcgg attgagcatt gacctgtccc accctatccc cccgccgatc acgggccaac
      301 tgaagatggg cggcaagaac cccgcggggg tggagatcaa cgccaacagc cgatacctca
      361 cgctgggcgg caagccgtgg gtgccggtaa tgggggaatt tcatttcacg cgatatccgc
      421 acgaggagtg ggagcgcgag ctgctgaaga tgaaagcggg cggcatcacg ctggtctcga
      481 cgtacgtctt ctggatccat cacgaagaag tgcagggcca attcgactgg agcggcgatc
      541 gcgatctgcg cgcattcctg caactgtgcg ccaagcatgg cctgctggcg tgggtgcgcc
      601 tgggcccgtg gtgccacggc gaagtgcgca acggcgggtt gccggattgg gtgctggccg
      661 agtgcggcaa aaacatccgc aagaatgatc cgaagttcat gggcttcacg cgcacgctgt
      721 atcagcagat cttcaagcag tgcgacggcc tactgtggaa agacggcggg ccgatcatcg
      781 gcgtgcagct ggaaaacgag atgtcgaaca atccagctta tttgctggcg ctgaagcgat
      841 tggcgcagaa cgtaggcttc gacgtgccgc tttattcggt caccggctgg ggcagcgtca
      901 aattcccggc ggatgaagtc atcccgctgt tcggcggcta tgccgacgga ttctgggaag
      961 gcggcagcac gtattcgaag aaggaccgca agcaattctt cttcacgcac gtgcgcgacg
     1021 actccggcat cggcgccgat ctgatgcccg cgacatggca cgccgcggcg gcggtggagc
     1081 tggatcgata tccctacgtc acctgcgaat gcggcggtgg gatggaaacg tcatacgcgc
     1141 gccggccatt aatgagcgcc ggcgacgtcg ccgcgttgtc gttcgtgaag atcggctccg
     1201 gctcgaacct tcccggttac tacatgtatc acggcgggcg aaacccggcg gggaaacttt
     1261 cgacgctgca ggaatcgcag gcgacgaatt acccgaacga catgcccgtc atccgctacg
     1321 acttccaggc gccgctgggc gagttcggcc aggtgcgcca atcgtacgat gcgctgcgaa
     1381 cgatccacgt gttcctcgcc gacttcggcg agcaactggc gacgatgccg gccatcttgc
     1441 cggccgagca accgaagtcg gttgacgaca cggcaacgct gcgctggtcc gcgcgatccg
     1501 atggccatcg cgggttcgtc ttcatcaaca actaccagcg cggcgaaccg ctgccggagc
     1561 ggcgggatgt gcagctaacg ctcaagctgg cggacggcga cctgacgttc ccgcagacgc
     1621 ccgcgaacgt gccaagcggc gcatatacga tttggccgtt caagctggac ctgaacggca
     1681 ccaacctgcg atacgcgacg gcgcagctgc tgtgccggat cgatgacggc ggtacaccga
     1741 tgttcgtctt cttcgccccc gcgggcaccg cggcggaatt ttcgatcgac ggcgtgggcg
     1801 tgattcatcc caatccacgc aatgatgtcg cgacgaccgt gcatgccgcc gagggtcgca
     1861 cggcgcgcgt gctgctgctc acgcacgaac aggcgatgca cgcgtacgtc ggtgacatcc
     1921 aaggcgcgcg acatctcgtc ctctcgcccg acggcaacgc gatattcgat ggc
//
LOCUS       DATIMH010001012         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3707832, whole genome shotgun sequence.
ACCESSION   DATIMH010001012 DATIMH010000000
VERSION     DATIMH010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3707832"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..552)
                     /gene="fliJ"
                     /locus_tag="VLI90_17630"
     CDS             complement(55..552)
                     /gene="fliJ"
                     /locus_tag="VLI90_17630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HSV16086.1"
                     /translation="MATFVFKLHGVLRHREMIEQEKQRAFAVAQAEKAAAQAEIKRLD
                     ETVQQALADLRANHLTGSLNLSFLAAHRRFMLAMQRQGLVLMQKVQDAQKKVDAAQTE
                     LAEAAKQRKIMDKLRERQHERWQLEISRKELAQLDEVAMQMSFQNNLAAGAMAGDVVE
                     PEALG"
     gene            complement(690..2015)
                     /locus_tag="VLI90_17635"
     CDS             complement(690..2015)
                     /locus_tag="VLI90_17635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HSV16087.1"
                     /translation="MPFRVVGTVQAISGLTIEASDLALPLGSLCRIHSFGSRTSTVEV
                     IGFQQDRTLLMPLTGTAGVSRGDRIENISAAPRIWCSEQLLGRVINGFGQPMDGKGAL
                     LLSESRRIDARSVAPLDRTNIREPIATSVRAIDALHTCGLGQRMGIFSGPGVGKSTLL
                     SSIAKNTSADISVIALIGERGREVQEFLHTSLGPDGLARCVMVVSTGDEAPLLRVRAA
                     KVATTIAEYFRDKGKHVLLLLDSLTRLCQAQRQIGLAAKEPPATKGFPPSVFALIPEI
                     LERAGKTATGSITGFYTVLVEGDDFNEPIPDAVKGITDGHLWLSRSLANRGHFPAIDI
                     LQSISRVRGDVTAADHGRMARRVLSLWATYQDIEDLVNIGAYVPGVNPEFDLAVQARP
                     KIVQFLQQEPTAPNTLEQSKKQLADLVTWIDQMERVLRAQKPKGAPPAK"
     gene            complement(2129..2836)
                     /locus_tag="VLI90_17640"
     CDS             complement(2129..2836)
                     /locus_tag="VLI90_17640"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HSV16088.1"
                     /translation="MGLIKSTAAPAALSAFSMRDIEAQATGLLRGARRAAEQLLVQAQ
                     VEAETLKRDAHAEGLAAGEAEGLAKGLEEGRRSGHQAALNETKGQLMAVWTALTAAVT
                     QLESARIELESAGVVEVIELAAGIARRVTKRQAAIDPAVLTANIRDAMNLAVHAADLR
                     IVINPAQRATLVDELPKLQLIWPTLQHVELVDDPAISPGGCRIMTRHGEVDAQIEGQL
                     DRVIADLMPRESPKNSE"
     gene            complement(2929..>3640)
                     /gene="fliG"
                     /locus_tag="VLI90_17645"
     CDS             complement(2929..>3640)
                     /gene="fliG"
                     /locus_tag="VLI90_17645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HSV16089.1"
                     /translation="QQVQTTPFSFLQKAESENLLTFIQDEHPQTIALILAHLPAQKAS
                     EIMVGLPSQKQVEVVKRIANMEQTNPEVIKEVERGLEHRLSDIVSQTFEKAGGVDTVA
                     EILNLADRSTEKGIMEGLEAEDPDLVEQIRRLMFVFEDILLVNDKGIQAVLKEVDNEE
                     LSLALKTASEELKQKIFKNMSERAAQLIAEDMQYMGPVRVSDVESAQQKIVDVVRRLE
                     DAGEIIIAGRGGEKEMVV"
BASE COUNT          612 a         1237 c         1057 g          734 t
ORIGIN      
        1 cgacggcgat gaaattgagc gccaggacga gcatgatcac ctgaatcaac ttcttcatcc
       61 gagcgcctcg ggctcgacca catcgcccgc catcgcgccg gcggcgaggt tgttctgaaa
      121 cgacatctgc atggccactt catccaactg ggccagttcc ttgcgcgaga tttccagctg
      181 ccagcgctcg tgctgtcgtt cgcgcagctt gtccatgatc ttccgctgct tagccgcctc
      241 ggccaactcg gtctgtgccg catccacctt cttctgcgcg tcctgaacct tctgcatcag
      301 cacgaggccc tgccgctgca tcgccagcat gaaacgccgg tgcgctgcca ggaacgacag
      361 gttcaaggag ccggttaaat gattcgcccg caggtcagcc agcgcctgtt ggaccgtttc
      421 gtccagccgc ttgatctcgg cctgagcggc ggccttctcg gcctgcgcca cggcgaaagc
      481 gcgctgcttc tcctgctcga tcatctcccg atgccgaagc acgccgtgaa gtttgaagac
      541 aaacgtcgcc atcagtcttt ccccctctcc cggtactccg ggagagggcc ggggtgaggg
      601 tcatttggaa tttcgacgcg ttctcgaaaa acaaacatca gcctcaccct aaccctctcc
      661 cggaataccg ggcgagggga ctaggagaat cacttcgccg gcggagcacc cttcggcttc
      721 tgcgcccgca acacccgctc catttggtcg atccacgtca ccagatccgc cagctgcttc
      781 ttcgattgtt ccagcgtgtt cggcgccgtc ggctcttgtt gaaggaactg cacgattttc
      841 ggccgggcct gcacggccag gtcgaactcc gggttcacgc ccggcacata cgccccgatg
      901 ttcaccaggt cttcgatgtc ctgatacgtc gcccagagcg agagcacgcg ccgcgccatc
      961 cggccgtggt cggccgccgt cacgtcgccg cgcacgcggc tgatgctttg caggatgtcg
     1021 atcgcgggga aatgcccgcg attggccagc gaccggctga gccagaggtg cccgtcggtg
     1081 atgcctttga ccgcgtccgg aatcggctca ttaaaatcgt cgccctcgac gagcacggtg
     1141 tagaatccgg tgatcgatcc tgtcgcggtc ttgcccgcgc gctcgaggat ttccggaatc
     1201 agcgcgaaga cgctcggcgg aaaccccttc gtcgccggcg gttccttcgc ggccagtccg
     1261 atctggcgct gggcctggca cagtcgcgtc aacgaatcga gcagcagcag gacgtgcttg
     1321 cccttgtcgc ggaaatattc ggcgatcgtc gtcgccacct tcgcggcccg cacgcgcaac
     1381 agcggcgcct catcgccggt gctgacgacc atcacgcatc gcgccagccc gtcggggccg
     1441 aggctggtgt gcaggaactc ctgcacttcg cgcccgcgtt caccgatcag cgcgatgacg
     1501 gagatgtcgg cactcgtgtt cttcgcgatg ctcgacaaca gcgtgctctt gccgacgccg
     1561 gggccagaaa aaatccccat gcgctggccg aggccgcagg tgtgcaacgc gtcgatcgcg
     1621 cgcacgctgg tggcaatggg ctcgcggatg ttcgtgcgat ccagcggcgc gacgctgcgc
     1681 gcgtcgatcc gccggctctc gctcagcaac agcgcgcctt tgccgtccat cggctggccg
     1741 aacccgttga tcacccgacc gagcaattgt tcagagcacc agatgcgcgg cgccgccgag
     1801 atattttcaa tgcgatcccc gcgcgacacg cccgcggtgc cggtcagcgg catcagcagc
     1861 gtgcggtcct gttgaaatcc gatgacttcg acggtcgagg tgcgcgagcc gaaactgtga
     1921 atgcggcaga gcgaccccag cggcagcgcc aggtcgctcg cttcgatcgt gagcccgctg
     1981 atcgcctgca ccgttccgac cacgcggaac ggcatgcaag actcaacggc atcgagttgg
     2041 gcggtcagat acggcatcgt tccaaagccg cttgcagctt agcgtctgtt cttttcgatc
     2101 gaaaaagacg ctaagccgca agcgccaatc actccgaatt cttcggcgat tcccgcggca
     2161 tcaaatccgc gatcacgcga tccagctgcc cttcgatctg cgcatccacc tcgccatgcc
     2221 gcgtcatgat ccggcagccg ccaggcgaga tcgcggggtc gtccaccagt tcaacgtgct
     2281 gcagcgtcgg ccagatcaac tgcagcttcg gcaattcgtc caccagcgtt gcgcgctggg
     2341 cggggttgat gacgatgcgc agatcggccg catgaacggc aaggttcatc gcgtcgcgaa
     2401 tgttggccgt cagcaccgcg ggatcgatcg ccgcctgccg tttggtcacg cgccgcgcga
     2461 tccccgcggc cagctcgatc acctcgacga cgccggcgga ttccagttcg atccgcgccg
     2521 attccagctg cgtcaccgcc gccgtcagcg cggtccacac ggccatcagt tgccccttcg
     2581 tctcattcag cgccgcctga tgcccgctgc gccggccttc ttctaaacct ttggccaatc
     2641 cctcggcctc tcctgccgcc agaccttcgg cgtgcgcgtc gcgcttcagc gtctcggcct
     2701 ccacctgcgc ctgtaccaac aactgctccg ccgcccgacg ggcgccgcgc agcaggcccg
     2761 tggcctgcgc ttcgatgtcg cgcatcgaaa acgccgacaa agcggcagga gcggcggtgg
     2821 acttgatcaa acccatggct catggatgca taatgatgaa tgcggaatgc agagtgaatg
     2881 cggattgccg ccgatttctg cattctgcat tcatcattct gcatttcctc aaacgaccat
     2941 ttccttctca ccaccgcggc ccgcgatgat gatctcgccg gcgtcttcca gccgacgtac
     3001 gacatcgacg atcttctgct gggccgactc cacgtcgctc acgcgcacgg ggcccatgta
     3061 ctgcatgtcc tcggcgatca gctgtgccgc gcgctcggac atgttcttga aaatcttctg
     3121 cttcagttct tcgctggccg tcttcagcgc caggctcagc tcctcgttgt ccacttcctt
     3181 cagcaccgcc tgaatgccct tgtcgttgac cagcaaaatg tcttcgaaga cgaacatcag
     3241 ccggcgaatt tgctcgacaa ggtccgggtc ctcggcctcc aggccttcca tgatcccctt
     3301 ctccgtcgat cgatcggcca ggttgagaat ctccgccacc gtgtccacgc cgccggcttt
     3361 ttcgaaggtc tggctgacga tgtcagagag gcgatgctcg agcccgcgct cgacttcctt
     3421 gatcacttcc gggttcgtct gctccatgtt cgcgatgcgc ttgacgactt cgacctgctt
     3481 ctggctgggc aagccaacca tgatttcgct cgccttttga gcgggcaagt gagcgaggat
     3541 cagcgcgatc gtctgcgggt gttcgtcctg gatgaaggtg aggaggtttt cggattcggc
     3601 tttttgcagg aacgagaacg gggtggtttg gacctgttgg
//
LOCUS       DATIMH010001013         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1689036, whole genome shotgun sequence.
ACCESSION   DATIMH010001013 DATIMH010000000
VERSION     DATIMH010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689036"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VLI90_17650"
     CDS             <1..241
                     /locus_tag="VLI90_17650"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSV16090.1"
                     /translation="PSKLGKKAELLALFEANRKKIADWVGTLSANDMAKPAPENLRSF
                     LPTVGHVGLLLPGHLNMHIGQIQVLRRKLGKPVMF"
     gene            322..900
                     /locus_tag="VLI90_17655"
     CDS             322..900
                     /locus_tag="VLI90_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16091.1"
                     /translation="MKTIVAAAAVVIAAGCSRDTWSYRAVPDTNLSRPNPMATRGTLV
                     LKGDSGTEAAPLSKQTVLMASWQHDSALYHHNVGRTLVIFIDGQPKPGQYWLTSENAV
                     LLTYSAYSAPARERVNLAGSIRVDRVEGNRIVADVAVQETSEIDSSEFIDHPWDAMYT
                     LPPFRLRGQHTFAVTTPADPIFEKSAVKWVAQ"
     gene            1010..>1575
                     /locus_tag="VLI90_17660"
     CDS             1010..>1575
                     /locus_tag="VLI90_17660"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HSV16092.1"
                     /translation="MIGLRSKILLAFGGLLAILLIVSAVSTFVFSRYSGATQLMLEQD
                     YSSVAACQDMEDAVEKSNSAIEKVLLGDARDVHVELEALARQFDDALAAQRQSLNLPG
                     EAEATQIAARLWAEYRQSLPAVSDAAKPLPQRRSEYERIVQPQADELLSATRRITELN
                     RTNATSTKGEAQRLAHSAMYALHVLTAAA"
BASE COUNT          291 a          478 c          537 g          269 t
ORIGIN      
        1 cccatcgaag ctggggaaga aggcggagct gctcgccctg ttcgaggcga accgcaagaa
       61 aatcgccgac tgggtcggca cgttgtcggc caacgacatg gcgaagccgg cgcccgagaa
      121 cctccgcagc tttctgccga cggttggtca cgtggggctg ctgctgccgg ggcacttgaa
      181 catgcacatt ggccaaattc aggtcctgcg acggaagctg ggcaagccgg tgatgttcta
      241 actggcgagg atcgggataa tcccgcgatt tcgattgaca gttaaccgtc ggtacggctc
      301 aatatccgta tgcattctct attgaagacc atcgttgccg ccgccgccgt ggtgatcgcg
      361 gcgggttgct cgcgcgacac ctggagctat cgggctgtgc ccgacacaaa cctgtcgcgc
      421 ccgaatccga tggcgacgcg cgggacgctc gtgctgaagg gtgactccgg caccgaggcg
      481 gcgccgctca gcaagcagac cgtgctgatg gcgtcgtggc agcacgactc ggcgctctat
      541 catcacaacg tgggccgaac gcttgtcatt ttcatcgacg ggcaacccaa gccgggccag
      601 tactggctga cgtcggagaa cgcggtgctg ctaacgtaca gcgcgtattc ggctccggcg
      661 cgcgagcgcg tgaacctggc gggttcgatt cgggtcgatc gcgtggaagg caaccggatt
      721 gtcgccgacg ttgcggtgca ggagaccagc gagatcgact ccagcgaatt catcgatcat
      781 ccgtgggacg cgatgtatac gctgccgccg ttccgcctgc gcggccagca cacgtttgcg
      841 gtgacgacgc cggccgatcc gatcttcgag aagtcggcgg tgaagtgggt cgcccagtaa
      901 ccaccgggcg agccgggcca tccccggccc gattctttcg cacgcacgcg cacgcaatga
      961 tgcagagacg cctcgtccac gagcgtctct gcgttcgtat gggcgtttaa tgatcggcct
     1021 tcgcagtaaa atcctgctcg cgttcggcgg gctgctggcg atcctgctca tcgtcagtgc
     1081 cgtcagcacg ttcgttttct cgcgatacag cggcgcgacg cagctgatgc tggagcagga
     1141 ctacagcagc gtggccgcct gccaggacat ggaagatgcg gtcgagaaga gcaacagcgc
     1201 gatcgagaag gtgctgctgg gcgacgcgcg cgatgtgcac gtggaattgg aggctctggc
     1261 ccggcagttc gacgatgcgc tggcggcgca gcggcagagc ctgaacctgc cgggggaagc
     1321 ggaggcgacg cagatagcgg cccggctatg ggcggagtat cgccagtcgt tgccggccgt
     1381 atcggacgca gccaagccgt tgccgcagcg gcgaagcgaa tatgagcgca tcgtgcaacc
     1441 gcaggcggac gagttgctct cggccacccg gcggatcacg gagctcaatc gcaccaacgc
     1501 tacgtcgacc aagggtgaag cgcagcggtt ggcgcacagc gcgatgtacg cgctgcacgt
     1561 gctgacggcg gcggc
//
LOCUS       DATIMH010001014         9416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_239946, whole genome shotgun sequence.
ACCESSION   DATIMH010001014 DATIMH010000000
VERSION     DATIMH010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239946"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VLI90_17665"
     CDS             complement(<1..282)
                     /locus_tag="VLI90_17665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV16093.1"
                     /translation="MTGEVDQLRAHYDAIPYLSAAFAQTHPDNLRAIAAVAGVDAPDV
                     TRCRVLEIGCATGRNLMPMAQTLPNSTFVGIDLSPRQIEAGNARVRELGL"
     gene            471..860
                     /locus_tag="VLI90_17670"
     CDS             471..860
                     /locus_tag="VLI90_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16094.1"
                     /translation="MAKKVLLVGHCGPDSSYLRMSIKRAVPEAEILMADDSDELSKSL
                     QRENIDLILFNRELGYGFDPDEGVEAIRILRQGRLNTKMMLVSNYPDAQQAAVAAGAT
                     LGFGKRELGTPRVTELLREALGVEVSK"
     gene            1113..1553
                     /locus_tag="VLI90_17675"
     CDS             1113..1553
                     /locus_tag="VLI90_17675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSV16095.1"
                     /translation="MQDTRWLVYAILAALCAGSINLFGKIGMEGLDPDLSTAVRSVVQ
                     AAFVVGFATVIGSWSKISTLHGRPLAIGMIILCGVAGGLSWIFAFRALKLADVSQVAP
                     IDKLSMPLGIILAVIILHERPTLVNWAGILLIAGGAYLATWPRR"
     gene            1611..2822
                     /locus_tag="VLI90_17680"
     CDS             1611..2822
                     /locus_tag="VLI90_17680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV16096.1"
                     /translation="MRIKGITRNVVVLGVVSFFTDVASEMLYPVMPLFLIGTIGASPA
                     LLGVIDGIAEGGSSVLRWLAGALSDRYRRRKPWVVWGYTISAFSKPVMGLAAIVGGWP
                     LFLVGRSTDRLGKSIRTSSRDALIADSTEPEYRGVAFGLHRAMDTCGAVLGPLIALSI
                     VLLWRGVPLQWLFFVALIPGLLSAGLATVAIKDIPHEAHPEATPPPILQSFPAPLWHL
                     IIAATVFSIGNSSDSFLILRSKELGLTFPQVILAFAVYNVVYAIAATPLGKLSDRAGR
                     KPIVIAGWTVYALVYFGFSIAHSTTAPWLLLAAYGLYQALSEGVTKALITDVVPKAQR
                     AGAIGLFYTATGLGQLIASVAAGALWGVRWFHGTVMASFILGSVCAAIAIPIVAGVRV
                     GVPPKLAEAQD"
     gene            2966..5500
                     /locus_tag="VLI90_17685"
     CDS             2966..5500
                     /locus_tag="VLI90_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16097.1"
                     /translation="MTLVRKQSRPSVLAGAALAALGASSAFAALPAFSGADGAGANVS
                     GGRGGIVYHVTRLDSSISDSGPGTLHYGVSDSNFKDANGNVIPRTIVFDVGGTIWLGR
                     NPGDTEGWDTTNSLSVGSNVTIAGQTAPGGINIAGGQVKLNGTGGTTPNSNTIFRNLT
                     LAAGYGTRKANSTSGYYDNYVYDAMDVNSNNVMVDHVSAFFATDETISCNELCNNTTI
                     QFTSMSQGQNYPQADAQGGGAYVGHAMGSLWSPGSNAKTSLLHNLYAHQGTRLPTVQT
                     VSGVLSKDANGNYIPPFNDVRNNVIYNWLGNAGYGSSGEPSNANFIGNYYKVGPGGDS
                     TTHNATDFSITQSSGGTTVFSGSSSTQIFQTSNVRLNLDRTTTNLTNSNFGSSAFQAS
                     ANSVPYNGVTDTAANAYNRVLNYVGANWQHRDAIDSRIVNQVRNGTGKITALDDASHG
                     YNSAGTYVTSGNDTEWNSLLALRGATNGGVGATGTYARAANFDSDADGMPDVWEVAHG
                     LNPAVADNNGDYDGNGYTNLEEYLNELAAFPSPSAVVFNNANANSRYAEIGNWTTGVF
                     EPSRYDEAQVNSGSAAIDAVGQHAGTLKIATNSGNSATLNVTAGWIDIAQSLLVGPGG
                     SGQVTQTGGIVHGGTSVVIGGATGIGSYALSGGTLSTPLLTKGAGGGTFAFTGGVLHA
                     DRVTFDLTNQGGTLAPGSDLALQLIAAAGMADINGNLPAIQSNIGDTHVNGNLTLAAG
                     TLQIELASLASFDTITVDNALSLGAALEVDLLNGYTPSPGDHWVIGTAGSISGDFGSV
                     SSGFSVQTVGGQLILIAVPEPASGLLALLPLVVLRRRRRARQRWCV"
     gene            5500..7134
                     /locus_tag="VLI90_17690"
     CDS             5500..7134
                     /locus_tag="VLI90_17690"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HSV16098.1"
                     /translation="MSIIVPPGHSHLPSRRESRRDDGRASGTSDAGDAIGGYPWTPDR
                     GDGTYRNPILFADYSDPDVVRVGDDFFLTASSFNCTPGLPILHSRDLVNWKIVNHAIR
                     QVPDARYERVQHGCGVWAPSIRHHDGRFWIFFPTPDEGIYVTTADDPRERWSEPRMVV
                     AGNGLIDPCPLWDDDGKAYLVFAYAHSRSGLKHQLRVCPMASDASRLLGDGQVVFRDE
                     ARHPTMEGPKFHKRDGWYYILAPAGGVATGWQVALRSRQVYGPYEPRIVLERGATSVN
                     GPHQGALIDSPDGREWWFVHFQEMLPYGRIVHLQPATWRDGWPMIGIDQDGNGIGEPV
                     AQHRKPMNSAGPPPCVPQSSDEFDSPQLGLQWQWHANHKPAWYSLDARPGWLRLNAQP
                     LPAPDRSLPEYPALLLQKFPAMRFATETRVELHADSLAVRAGLAVVGLSCASLSTRRE
                     GQLQQVVLQINGENVAAADVSLGAVTLRIHVSDGGACRFEFTETGSVAQAIGDTFKAT
                     EGKWIGAKIGVFCASDSDVATDAHADFDYVRLSRAD"
     gene            complement(7424..8422)
                     /locus_tag="VLI90_17695"
     CDS             complement(7424..8422)
                     /locus_tag="VLI90_17695"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HSV16099.1"
                     /translation="MTCSFEPLENRTMFSVVSVATLDPNASEAGQDPASFFVYRQDRA
                     PIATRVYFTVGGTATSPLNSLKAGFADYTGIHAPPPPVIQAFAGTARAAAAIGIVGGN
                     TGYVDIPAGQTYAVVTITPVDDTRVESLETATFTLKPDAEYQIGSSSTVALSIHDNDG
                     PVVANDIADSYLRDGNSANTNFGHSTHLEVKKGPIGFNRATLIKFDLSHLDPLAGYGH
                     VTLKVFGSLSNTNQKNVVTNVLPFADSSWSETDITWNHRPIIFTRGDSTLSTATIIDN
                     TPRWYTWDVTSFVKSELAAGHTACTLVLRNMNDSDPIATFNSREASGNHPQLVFTA"
     gene            complement(8571..9047)
                     /locus_tag="VLI90_17700"
     CDS             complement(8571..9047)
                     /locus_tag="VLI90_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16100.1"
                     /translation="MDELRSAVQSAARRPEIRPLVRDLYARLRSRIDERRPICVASGR
                     CCRFDEYGHRLYVTTIELAAFVSELQATTPPPIITPQPGGCLFQIGKLCGVHAIRPFG
                     CRVFFCDPTAEAWQTEQYEQFHAELKRLHDELAVPYFYVEWRQALTALSLGDSPHV"
     gene            complement(9083..>9416)
                     /locus_tag="VLI90_17705"
     CDS             complement(9083..>9416)
                     /locus_tag="VLI90_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16101.1"
                     /translation="RMFCKNGDGWSKCEREVAKTNAKGNRCPKSKSLIAAGDSQREIF
                     HALIEAFDEYGFPDPKRVTSDDLPLIASRIALIEQLAPFAMDQVNADAFAEAHKDLTN
                     MLVKASQP"
BASE COUNT         1722 a         2902 c         3037 g         1755 t
ORIGIN      
        1 cagccccagt tcccggacgc gggcattgcc ggcttcaatc tgccgcggcg agaggtcgat
       61 acccacaaag gtgctgttcg gcagggtctg cgccatcggc atcaggttgc gtccggtcgc
      121 gcagccaatc tcgagcacgc ggcagcgcgt cacatccggc gcgtccaccc ccgccacggc
      181 ggcgatggcg cgaaggttgt ccggatgcgt ttgcgcgaat gcagcgctga ggtatggaat
      241 cgcgtcgtag tgcgcgcgga gttgatcgac ctcacccgtc atgttggcga aatgatcagc
      301 gagtgcgtgc gaaatgcaag cctgcggcgt gaacaggcgg tccgttccgg caggactata
      361 attttgtccg ccagcagcac ccagctcagc cgcgagaaaa ggtaccgatg tgtctgcccg
      421 ccgaggtgcg cggcttgctg tcctcatagg aacggatgga gggatttgtc atggcgaaga
      481 aggttttgtt ggtaggtcac tgcggtcccg acagcagcta tctgcggatg tccatcaagc
      541 gcgccgtgcc cgaggcggag atcctgatgg cggatgactc ggatgagctc agcaagtctc
      601 tccaacgcga aaacatcgac ctgatcctgt tcaaccgcga gctcggttac ggcttcgacc
      661 cggacgaagg cgtcgaggca atccgcatcc ttcgccaggg ccgactgaac acgaagatga
      721 tgctggtgag caattacccc gacgcccagc aagccgcggt ggccgccggg gcgacgctgg
      781 gtttcggcaa gcgcgagctg ggcacgccgc gcgtgacgga gctgctgcgc gaggcgctgg
      841 gcgttgaagt gtcgaaatga attgaaccat tccgatcccg tctcccggcg taccgctgac
      901 gacccggatg ctttttgcgc ggcgcagaat tcgggtcgtc ggagtacctc cgaacccggc
      961 gtggtcggtt cctaaccatt cctacaaaag tgtcgggtcc tcagtgacgt accgggagag
     1021 gaggtaatac gaagcccgtg cgtgcggcgc aattctgtga aatctttggt ccaaactgtc
     1081 tggtccgcgc ctcttcgtgg taaatcatat cgatgcaaga cacacgctgg ctcgtctacg
     1141 cgatccttgc ggcattgtgc gcggggtcga tcaatctctt cggaaaaatc ggcatggagg
     1201 ggctcgaccc ggacctgtcc accgcggtgc gcagcgtggt gcaggcggcg ttcgtcgtcg
     1261 gctttgcgac cgtcatcgga tcgtggtcaa agatctcgac cttgcatggc cggccattgg
     1321 cgatcgggat gatcatcctc tgcggcgtgg ctggggggct atcgtggatc ttcgcctttc
     1381 gcgcgctcaa gctggcggat gtgtcgcagg tcgcgccgat cgacaagctg tcgatgccgc
     1441 tcggcattat cctggccgtc atcatcctgc acgaacggcc aacgctggtg aactgggccg
     1501 ggatcctgct gatcgccggc ggggcctatc tggcgacctg gccgcggcgc taagcgccac
     1561 atcaaccacc atccggtatc atccacccag ccgcgatctt gaactgcaac atgcgaatca
     1621 aaggcatcac gcgcaacgtc gtcgtgctcg gtgtggtcag ctttttcacg gacgttgcgt
     1681 ccgagatgct ttacccggtc atgccgttgt tcctgatcgg gacgatcggc gcgtcgccgg
     1741 cgctgctagg ggtcatcgac ggtattgccg agggcggcag cagcgtgctg cgctggctgg
     1801 cgggcgcgct gtccgatcgc tatcgccggc gaaagccgtg ggtcgtgtgg gggtacacga
     1861 tctcggcgtt cagcaaaccc gtcatgggcc tggcggccat cgtgggcggt tggccgctct
     1921 ttttggttgg ccgatcgacc gatcgcctgg gcaagtctat ccgcacctcg tctcgcgacg
     1981 cgttgatcgc cgactcgacc gagccggaat accgcggcgt agcgtttggc ttgcaccgtg
     2041 caatggacac ttgcggcgcg gtgctcgggc cgctgatcgc gctgtcgatc gtgctgctct
     2101 ggcgcggcgt gccgcttcaa tggttgtttt tcgtcgcgct gattccaggg ttgctcagcg
     2161 ctgggttggc gaccgtcgcc attaaagata ttccgcacga ggcccatccg gaggcgacac
     2221 cgccgccgat tctgcagtcg ttcccggcgc cgctgtggca cctgatcatc gccgcgacgg
     2281 ttttttccat cggcaacagc agcgactcgt tcctgatcct gcgcagcaag gaactggggc
     2341 tgacgttccc gcaggtgatt ctggcgttcg cggtgtacaa tgtcgtgtat gcgattgcgg
     2401 caacgccgct ggggaaactt tcggaccgcg cgggtcgcaa gccgatcgtc atcgcaggat
     2461 ggactgtcta cgcgctggtg tacttcggct tctccatcgc ccattcgacg acggcgccgt
     2521 ggttgttgct ggcggcgtac ggactgtatc aagcgctgag cgaaggcgtg accaaggcgc
     2581 tgatcacgga cgtggtgccg aaggcgcagc gcgccggcgc cattggcctg ttctacaccg
     2641 cgacgggctt gggtcagctg atcgccagcg tggcggcggg ggcgctgtgg ggcgtgcggt
     2701 ggttccacgg cacggtcatg gcgagcttta ttctgggatc ggtgtgcgcc gcgattgcca
     2761 tcccgatcgt ggctggcgta cgggttggcg tgccgccgaa gcttgccgaa gctcaggact
     2821 gatccacgac cccgagccgt tcagaaatcc aaaagattcg cgccaatttc gaaattcacc
     2881 ccgcttgctc ttctgatacc tttcgattcc ggcggggccg tgaagcggaa ccggtcgtca
     2941 gagttgcatg gcaggcggag gtgaaatgac cttagtgcgg aagcagtctc gaccgagcgt
     3001 ccttgctggc gctgcattgg cagcgctcgg cgcctcgtcg gcatttgcgg cgctgccggc
     3061 gttttctggc gcggatggcg cgggcgcgaa tgtaagcggt ggccgcgggg ggatcgttta
     3121 tcacgtcacc cggctcgact ccagcatcag cgacagcggc cccggcacgt tgcattacgg
     3181 cgtgtcggac tccaacttca aggacgccaa cggcaacgtc atcccgcgga caatcgtctt
     3241 cgacgtcggc ggcaccatct ggctcggccg aaatcccggt gataccgaag gctgggatac
     3301 gaccaactcg ctgtcggtgg gttccaacgt cacgattgcc ggccagaccg caccgggcgg
     3361 catcaacatc gccggtgggc aggtgaagct gaacggcacc ggcgggacga cgccgaattc
     3421 gaacacgatt tttcgcaacc tcacgctggc cgccgggtat ggtacgcgca aggcgaactc
     3481 cactagcggg tattacgaca actatgtgta tgacgcgatg gacgtgaact ccaacaacgt
     3541 catggtcgat cacgtgtcgg cgttcttcgc caccgacgag acgatctcct gcaacgagtt
     3601 gtgtaacaac acaacgatcc aattcaccag catgtcgcag ggccagaatt acccacaggc
     3661 cgacgcccag ggcggcgggg cgtatgtggg gcacgcgatg ggctcgctct ggtcgccagg
     3721 atcgaatgcc aagaccagct tgctgcataa cctgtatgcc caccaaggca cgcgcctgcc
     3781 gaccgttcag accgtgtccg gcgtgctgtc gaaggatgcc aacggcaact acatcccgcc
     3841 gttcaacgac gttcgcaaca acgtcatcta caactggctc ggcaacgccg gttacggcag
     3901 cagcggtgaa ccgagcaacg cgaactttat cggcaactat tacaaggtcg gtcccggcgg
     3961 cgacagcacg acgcacaacg ccaccgattt ttccatcacg caatccagcg gcgggacgac
     4021 cgtcttcagc gggtcgtcct ccacgcagat ctttcagacc agcaatgttc gcctgaacct
     4081 ggaccgcacg acgaccaatt tgaccaacag caatttcggt tcatccgcgt tccaggccag
     4141 cgccaacagc gtgccttaca acggcgtgac cgacaccgcg gccaacgcct acaaccgcgt
     4201 gctgaattac gtcggcgcca attggcagca tcgcgacgcg atcgacagcc ggatcgtcaa
     4261 ccaggtgcgc aacggcaccg ggaagatcac ggcgctggat gatgcgagcc acggctacaa
     4321 cagcgccggc acctatgtga ccagcgggaa tgacaccgag tggaactccc tgctcgcgct
     4381 tcgcggtgcg accaacggcg gggtgggcgc aaccggcact tacgcgcgtg ccgccaactt
     4441 cgacagcgat gccgatggaa tgcccgatgt gtgggaagtc gcgcatggtc tcaatcccgc
     4501 agtcgcggac aacaacggcg actacgacgg caatggttac acgaacctcg aagagtacct
     4561 gaacgaactg gcggcatttc cctcgccgtc ggcggtcgtg ttcaacaacg ccaacgccaa
     4621 cagtcgctac gccgagatcg gcaactggac gaccggcgtg tttgagccgt cgcgatatga
     4681 cgaagcgcag gtgaacagcg gatcggccgc gatcgacgcg gtgggtcaac acgcgggcac
     4741 gctgaagatc gcgaccaaca gcggcaatag tgcgacattg aacgtcaccg ccggttggat
     4801 tgacatcgcg cagagtctgc tggtcggacc cggtggtagc gggcaagtca cgcagaccgg
     4861 cggcatcgtt cacggcggga cgtccgtcgt catcggcggg gcgaccggca tagggagcta
     4921 cgcgctaagc ggcggcacgc tcagcacgcc gctcctcacc aaaggcgccg gcggcgggac
     4981 gttcgcgttt accggtggcg tgctgcacgc cgatcgcgtg acgttcgatc tgaccaacca
     5041 aggcggcacg ctggcgccgg gcagcgacct cgccctgcag ttgatcgccg ccgcgggaat
     5101 ggcggacatc aacggtaatc tgccggcgat ccagagcaac atcggcgaca cgcacgtcaa
     5161 cggaaatctc accttggccg cggggacgct gcagatcgaa ctcgcctcgc tcgccagttt
     5221 cgatacgatc acggtggaca acgcgctgtc gctcggcgcc gcgctggagg tcgatctgct
     5281 caacggctac acgcccagcc ccggcgatca ctgggtgatc ggcaccgcgg gcagcatcag
     5341 cggcgacttc ggctccgtct cgagcggatt cagcgtgcag accgtgggcg ggcagttgat
     5401 tctcatcgcg gtgcccgagc cggcctcggg gctgctggcg ctgttgccgt tggtggtctt
     5461 gcgtcgccgc cgccgagcac ggcaacggtg gtgcgtctga tgagcatcat cgtcccgcca
     5521 ggccattcgc atttgcccag tcgtcgcgag agccgaagag acgacggccg cgcttcgggc
     5581 acgtccgacg cgggcgacgc gatcggcggt tatccatgga cgcccgatcg cggcgacggc
     5641 acatatcgca acccgatcct cttcgccgac tattcggatc ccgacgtcgt tcgcgtcggt
     5701 gacgatttct ttctcaccgc gtccagcttc aactgcacgc cggggctgcc gatccttcat
     5761 tcgcgcgatc tggtgaactg gaagatcgtc aaccatgcca ttcgccaggt tcccgatgcg
     5821 cgctacgagc gcgtgcagca cggctgcggc gtgtgggccc cgtcgatccg ccatcacgac
     5881 gggcggttct ggatcttctt tcccacgccg gatgaaggca tttatgtgac caccgcggac
     5941 gatccgcgcg agcgatggag cgagccgcgc atggtcgtcg cgggcaacgg gctgatcgat
     6001 ccatgtccgc tgtgggatga cgacggcaag gcatatctcg tcttcgcgta tgcgcattcg
     6061 cgcagtgggt tgaagcacca gctgcgcgtc tgtccgatgg cgtccgatgc atcgcgcttg
     6121 ctgggcgatg gtcaggtcgt ctttcgcgat gaggcccggc atccgacgat ggaagggcca
     6181 aagtttcaca agcgggacgg gtggtactac atcctcgccc ccgcgggcgg cgtggcgacg
     6241 ggctggcaag tggcgctgcg gtcgcggcag gtatacgggc catacgagcc tcgcatcgtg
     6301 ctcgagcgcg gggcgaccag cgtaaacggc ccgcaccagg gtgcgctgat cgacagcccc
     6361 gacgggcgcg agtggtggtt cgtgcatttt caggagatgc tgccgtacgg gcggatcgtg
     6421 caccttcaac ccgcgacgtg gcgcgacggc tggccaatga tcggcatcga tcaggatggc
     6481 aacggcattg gtgaacccgt cgcgcagcac agaaagccga tgaacagtgc ggggccgccg
     6541 ccgtgcgtgc cgcagagcag cgatgaattc gactcgccgc agcttggcct gcagtggcaa
     6601 tggcacgcga atcacaagcc cgcgtggtac tcgctggacg cgcggccggg atggctgcgc
     6661 ttgaacgcgc aaccgctgcc ggcgccggac cgcagcttgc cggagtatcc ggcgctgctg
     6721 ctacagaaat tcccggcgat gcgattcgca accgaaacgc gggtcgagct gcacgccgat
     6781 tcgctggcgg tgcgcgccgg cctggcggtc gttggtctct cgtgcgcatc cctcagcaca
     6841 aggcgggagg gacaactgca gcaggtcgtg ctgcaaatca atggcgaaaa cgtcgcggcg
     6901 gcggatgtgt cgttgggcgc ggtgactcta cgaatacacg tcagcgatgg cggcgcctgt
     6961 cggttcgaat tcaccgagac cggcagcgtc gcccaggcga tcggcgacac gttcaaggcg
     7021 accgaaggaa agtggatcgg cgcgaagatc ggcgtcttct gcgccagcga ttccgacgtg
     7081 gcaaccgacg cccacgcgga tttcgattac gttcgcctca gtcgcgccga ctagagctgc
     7141 tcgtcacggt gcacgcgcgc ttcgtcatcc tccagcgcag gcgtactcac cggagtcgaa
     7201 ggatcttgcc gacgagtcga tgaacctttg attagggaag acggcgccgc agtcgcttgc
     7261 ggtttcgcag acggacaaaa cgcggtcact ggtctgcgaa accgcaagcg actgctgtac
     7321 cgtcttcgga tctgaacgtt cactcgcttc tcggcgagac ccttcgaatg cgcacgagta
     7381 cgtcctgcgc tccggatggc gcgcgcacgc atgatcacac tcattacgcc gtgaacacca
     7441 actgcgggtg attgccgctg gcttcgcgcg agttgaacgt cgcaatcgga tcgctgtcat
     7501 tcatgttccg caagaccagc gtgcaggccg tgtgtcccgc cgccagctcc gacttcacga
     7561 agctcgtcac gtcccaggtg taccacctcg gcgtgttgtc gatgatcgtc gcggtggaca
     7621 acgtactgtc gccgcgtgtg aagatgatcg ggcgatggtt ccacgtgatg tccgtttcgc
     7681 tccagctgga gtcggcgaac ggcagcacgt tggtgaccac gttcttctgg ttcgtgttgc
     7741 tgagcgaccc gaacaccttc agcgtcacgt gcccataacc agccagcgga tccaagtggc
     7801 tcaagtcgaa cttgatcaac gtggcgcgat taaaaccgat tggccccttc ttcacttcaa
     7861 gatgtgtgga gtgaccgaag ttggtgttgg cggagttgcc gtcgcgcagg tacgagtcgg
     7921 cgatgtcatt ggcgacgacg ggcccgtcgt tgtcgtggat cgacagcgcc acagttgaag
     7981 acgaaccgat ttggtattcc gcgtccggct tcagcgtgaa tgtcgccgtc tcgagcgatt
     8041 caacccgggt atcatcgacc ggcgtgatgg tgacgacggc ataggtctgg cccgcgggga
     8101 tgtccacgta cccggtgttc ccaccaacaa tgccgatcgc cgcggcggcc cgcgcggtgc
     8161 cggcaaacgc ctgaatgacc ggcggcggtg gagcatggat tccagtgtag tcggcgaaac
     8221 cagctttaag tgagttcaat ggcgaggtgg ccgtgccacc aacggtaaag tacacccggg
     8281 tcgcaattgg cgcgcgatcc tggcgataga cgaaaaagct ggccgggtcc tgacccgctt
     8341 cgctggcgtt ggggtccagc gtggcaacgg aaacaacgct gaacatcgtg cgattttcaa
     8401 gcggttcaaa cgagcaggtc atgagaagct ccggagggtt atcaatcgtg cggaagagac
     8461 gcagttgaaa tacttgaggc ggcggcgatt catccggcca caccaacgat tcgcgattag
     8521 cagtcgaatc ttgcgcagcc ctaagtgtcg cgaccgcgtt tacacagccg ctaaacgtgg
     8581 ggagaatcgc cgagcgatag cgccgtaagc gcctgccgcc actcgacgta gaagtacggc
     8641 accgcaagct catcgtgcag tcgcttcagt tcggcgtgga attgctcgta ctgctcggtc
     8701 tgccaggcct cggcggtggg atcgcaaaaa aagacgcggc aaccgaaggg gcggatggcg
     8761 tgcacgccgc agagcttgcc gatttggaac agacacccgc cgggttgcgg cgtgatgatc
     8821 ggcggcgggg ttgtggcttg gagttcgctg acgaacgcgg ccagttcgat cgtcgtgacg
     8881 tacaggcggt ggccgtattc gtcgaatcgg cagcaccggc cggaggcgac gcagatgggt
     8941 ctccgctcat cgatccgcga ccgcaggcgc gcgtagagat cgcgcacgag cggccggatc
     9001 tccggccgac gcgccgcgga ttgcacggcc gatcgcagtt cgtccatcac agccgcgaat
     9061 catagcacgc catcggccga catcacggct ggctggcctt gaccagcatg ttggtcaaat
     9121 ccttgtgcgc ctcggcgaac gcgtcggcgt tcacctgatc catcgcgaac ggcgcaagct
     9181 gctcgatcag cgcaatgcgg ctggcgatca acggcagatc gtccgacgtc actcgtttcg
     9241 gatcaggaaa tccatattca tcaaaagctt cgatgagagc gtgaaagatc tcccgctggc
     9301 tgtcgcctgc ggctattaaa gatttgctct tcggacaccg atttcccttc gcgtttgtct
     9361 tcgcgacttc gcgttcgcat ttactccagc catcgccatt cttgcaaaac atccgc
//
LOCUS       DATIMH010001015         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1109662, whole genome shotgun sequence.
ACCESSION   DATIMH010001015 DATIMH010000000
VERSION     DATIMH010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109662"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VLI90_17710"
     CDS             <1..237
                     /locus_tag="VLI90_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16102.1"
                     /translation="TGEIAVIVILTYIVGLGQLTHIIAGSVEVLYLVATGGMSEWTYL
                     GGYMLPTLIGNVIGGTALVSALNHAQVVAGHGNK"
     gene            complement(451..1536)
                     /locus_tag="VLI90_17715"
     CDS             complement(451..1536)
                     /locus_tag="VLI90_17715"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HSV16103.1"
                     /translation="MSNALKIKPAGGALDFLSLGALVHRLDPGIIPFRKAHECAIHVS
                     GGEFNVAANLSDAFGMNTGICSAMVKYPIGDLIAERVRAMGVKPFYKYFEHNGVTGPN
                     MAAVYSDRGHGIRPPVVFYNRSNEAGALLKPGDFNWKEIFGAGVRWFHSGGIFAALSE
                     TTGELIIEAMQAAKDAGVVTSFDLNFRAKLWKIFGGENRAVSVIDRIVKNVDVLVGNE
                     EDLQKGLGIPGPEMAAKSKLDPSAFFGMMDNVLKKHPHIKVVATTLREVHSTNHHGWS
                     AVAWMDGKTYQAPEAELHVHDRVGGGDGYAAGFFYGLMNGDSPETAVRLGWAHGALLT
                     TYPGDTTMATLDEVRAFAAGGSARIQR"
     gene            complement(1614..>2012)
                     /locus_tag="VLI90_17720"
     CDS             complement(1614..>2012)
                     /locus_tag="VLI90_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16104.1"
                     /translation="LNLGGIALMWRGGCIIRSRFLGKIKEAFDTNPKLNNLLLDDFFS
                     STLNKYQESWRKGIISAIEYGVPTPAFSTALAFYDGYRSERLPANLLQAQRDFFGAHT
                     YERVDKPRGQFFHTNWTGRGGRVSSGTYNV"
BASE COUNT          374 a          565 c          663 g          410 t
ORIGIN      
        1 accggcgaaa tcgcagtgat cgtcatcctc acctacatcg ttggactcgg ccagctcacg
       61 cacatcatcg ccggctcggt ggaggttctc tatcttgtag cgacgggcgg gatgtcggaa
      121 tggacgtatc tcggcggcta catgctgccg acgctgattg gcaatgtgat tggggggacg
      181 gcgctggtat cggcgcttaa tcacgcccag gtggttgccg gacacggcaa caagtaaaag
      241 aggaggccgg ctttcgccgg cgctcgaagt gtattctgtt ttcgaagagc tttttagaca
      301 gcggctccga ttttcgcagg aagcttgcct ggctggtttt agcagacggc agcggaagaa
      361 cagcatggcc gacttcgtcg gctggtcgga taccatccga ctgcagattt ccaatctgcc
      421 ccacaattgc tcagcagaac ggggacaggg ttacctctga atgcgggcgc tacctccggc
      481 ggcgaaggcg cgaacttcgt cgagggtcgc catcgtggtg tcgcccggat acgtggtcag
      541 caaagcgccg tgagcccaac cgaggcggac tgccgtctca ggactgtcgc cgttcatcag
      601 gccatagaag aagcccgccg catagccatc gccgccgccc acgcgatcgt ggacgtgcaa
      661 ctcggcttcc ggggcctggt acgtctttcc atccatccag gcgacggcgc tccagccatg
      721 gtggttcgtc gagtgaactt cgcgaagggt ggttgctacc actttgatgt gcgggtgctt
      781 tttgagcacg ttgtccatca tgccgaagaa cgcgctgggg tcgagcttcg acttggctgc
      841 catctccggc ccggggatgc caaggccctt ctgcagatcc tcttcgttgc cgacgagcac
      901 gtccacgttc ttcacgatac ggtcaatgac actgacggcc cggttctcgc cgccgaagat
      961 cttccacagt ttggcgcgga agttgaggtc gaaagaggtc accacgccgg cgtctttcgc
     1021 cgcctgcatg gcttcgatga tgagctcgcc ggtggtttcg gagagcgccg cgaagatgcc
     1081 gcccgaatgg aaccagcgga cgcccgcgcc gaagatctcc ttccagttga aatcgccggg
     1141 cttgaggagc gcgccggctt cattggaccg gttgtagaac acgaccggtg ggcggatgcc
     1201 gtgtccgcga tcgctgtaga cggccgccat attggggccg gtgacgccgt tgtgctcgaa
     1261 gtacttgtag aagggcttga cgcccatggc gcggacgcgc tcggcgatga ggtcgccgat
     1321 cgggtacttg accatggcgc tgcagatgcc ggtgttcatc ccgaatgcat cggaaagatt
     1381 ggcggcgacg ttgaactcgc ctccgctgac gtggatggcg cactcgtgcg ccttgcggaa
     1441 cggaataatg ccggggtcca gacggtggac gagggcgccg agagacagga agtcgagggc
     1501 gccgccggcg ggtttgattt tgagtgcgtt gctcatttgg gttccttaag aatcaaaaga
     1561 acaggccaaa gccagccttg cgaaggaggc tgaagcctgt gttacggcct gcgttagacg
     1621 ttatacgttc cggaggaaac tcgaccgccc cgaccggtcc aattcgtgtg gaagaattgg
     1681 ccgcgcggtt tgtcgacgcg ttcgtaggtg tgggcgccga agaagtcgcg ctgggcctgc
     1741 agcaggttag ccggcagtcg ctccgagcga tagccgtcgt agaaggcaag cgccgtggag
     1801 aatgcggggg tggggacgcc gtactcgatg gcggagataa ttcccttgcg ccaggactcc
     1861 tgatacttat tgagtgtgct gctgaagaag tcgtcgagca gcaggttgtt gagcttggga
     1921 ttcgtgtcga aggcttcctt aatcttgccg agaaaccggc tgcggatgat acagcccccg
     1981 cgccacatca gcgcgatccc gccgaggttg ag
//
LOCUS       DATIMH010001016         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_821788, whole genome shotgun sequence.
ACCESSION   DATIMH010001016 DATIMH010000000
VERSION     DATIMH010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821788"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VLI90_17725"
     CDS             complement(<1..472)
                     /locus_tag="VLI90_17725"
                     /inference="COORDINATES: protein motif:HMM:NF019008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HSV16105.1"
                     /translation="MLINFAKRGIVFAGVATLALAHLAAEPAARPTTRPGGARASVNL
                     NHEMESMGRAFKALQRQINDSAQNASSLALVGELQQHTMLAKSAAVPKNVATPNEDPA
                     QSAADYHGMMLNLLRQELDLEEQLLDNQNDKAAATLKSIDELQDQGHKEFQPKKK"
     gene            complement(487..1800)
                     /locus_tag="VLI90_17730"
     CDS             complement(487..1800)
                     /locus_tag="VLI90_17730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HSV16106.1"
                     /translation="MTLVEKITAHSAVVGVIGLGYVGLPLLAAFHRAGFPVIGFDNDA
                     KKIEALGRGENYLKHLGESLVADMAKVGRFDVTADASRLREADAILICVPTPLGKHLE
                     PDLSFVQRTADDVARALRPGQLIVLESTTYPGTTREIVLPRLSARGLTLGVDFFLAFS
                     PEREDPGRKDFNTQTIPKLVGGIDAASGDVAVNLYRQAIQQVVPVSSAEVAEAAKLLE
                     NVYRAVNIALVNEMKTVLSAMGIDVWEVIRAAATKPFGYQPFYPGPGLGGHCIPIDPY
                     YLTWKAREVGLPTRFIELAGEINHAMPQYVIERLVLALNQRGKAVKGSRILVLGLAYK
                     PDLDDVRESPSFELIEKLVELGASIDYSDPHVPATHRMRKYGDLGMRSVPVTPDALKN
                     YDAVLVATHHKAFDWQMIADHSTLIVDTRNALRDVKGRRDHIVPA"
BASE COUNT          323 a          652 c          567 g          390 t
ORIGIN      
        1 ttttcttctt cggttgaaat tccttgtgcc cctggtcctg cagctcgtcg atcgacttca
       61 gcgtcgccgc cgccttgtcg ttctggttgt ccagcagctg ctcttccaaa tccaactcct
      121 gccgcagcag gttcagcatc atgccgtgat agtcggcggc ggactgggcg ggatcttcat
      181 tcggtgtcgc cacgttcttc ggcaccgccg cgctcttggc cagcatcgtg tgctgctgca
      241 actcgcccac cagcgccagc gatgacgcgt tctgcgctga atcgttgatc tgcctctgca
      301 acgccttgaa cgcccggccc atcgattcca tttcgtgatt gagattgacc gacgcgcgag
      361 caccaccggg acgcgtggtt ggccgggccg ccggctccgc cgccaggtgc gccagcgcga
      421 gcgtcgcgac gcccgcgaaa acaattccac gttttgcaaa gttgatcagc attctcttcc
      481 ttacgcttac gccggcacga tgtggtcgcg ccggcctttc acatcgcgca gtgcattacg
      541 cgtatcgacg atcaacgttg aatgatcggc gatcatctgc cagtcgaacg ccttgtggtg
      601 cgtcgccacc agcaccgcgt cataattctt cagcgcatcg ggcgtaactg ggacgctgcg
      661 catgcccagg tcgccgtact tgcgcatgcg atgcgtcgcg ggcacgtggg ggtcgctgta
      721 atcgattgaa gcgccaagct cgaccagctt ctcgatcaac tcaaaactcg ggctctcgcg
      781 cacgtcatcc aagtcgggtt tgtacgccag ccccagtacg aggattcggc tgcctttgac
      841 cgccttgccg cgctggttca gcgccagcac cagccgctcg atcacgtact gcggcatcgc
      901 gtggttaatc tcccccgcga gttcgatgaa ccgcgtcggc aggccgacct cgcgcgcttt
      961 ccacgtcagg taatacgggt cgatcggaat gcagtgcccg cccagcccgg ggccgggata
     1021 gaacggctga tagccaaacg gtttggtcgc ggccgcgcgg atgacttccc acacgtcgat
     1081 gcccatcgcc gacagcaccg tcttcatctc gtttaccagc gcgatgttca ccgcgcgata
     1141 gacgttctca agcaatttag ctgcttctgc tacttccgca ctggaaaccg gcacgacctg
     1201 ctggatcgcc tggcgataca ggttcaccgc gacatcgccg ctggcggcgt cgatgccgcc
     1261 gaccagcttg gggatcgtct gggtgttgaa atccttccgg ccgggatctt cgcgctcggg
     1321 cgaaaacgcg aggaagaaat cgacgccgag cgtcagcccg cgcgccgaca gccgtggtag
     1381 tacaatctcg cgcgtcgtgc cggggtacgt cgtcgattcc agcacgatca attgtccggg
     1441 ccgcagcgcc cgtgcgacgt catccgccgt gcgctggacg aagctcaggt ccggctccag
     1501 gtgcttgccc agcggcgtgg gcacgcagat gaggatcgca tccgcctcgc gcaggcgtga
     1561 cgcatccgcc gttacatcga accgccccac cttcgccatg tccgcgacca gcgattcgcc
     1621 cagatgtttc aggtaattct cgccccggcc gagcgcctcg atcttcttcg cgtcattgtc
     1681 gaacccgatg acgggaaacc ccgcgcgatg aaacgccgcc agcagcggca ggccgacgta
     1741 accgagcccg atgacgccaa caacggcgct gtgagcggta atcttttcca caagggtcat
     1801 ctggcgcggt agtttaatcg aagtggtgaa ggttggcgcg ttgcgccgct ttgcgccgcg
     1861 aacccgcgca tagccgccat cgtttagccg ccgcggcctc gcggcgcttt tctcagtccg
     1921 cgccgcgagg cc
//
LOCUS       DATIMH010001017         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4572868, whole genome shotgun sequence.
ACCESSION   DATIMH010001017 DATIMH010000000
VERSION     DATIMH010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4572868"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3218)
                     /locus_tag="VLI90_17735"
     CDS             complement(<1..>3218)
                     /locus_tag="VLI90_17735"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV16107.1"
                     /translation="LVIGSASQRAVVELTDAIDNGHRGGSGKEALYLLDVSGHGARIL
                     NGSTLVLNHLDAYSISGNAFINLNSLFPAGQNRVAYDGGFLSKSMADIGTAWKVDADG
                     NWSDASNWNVASPNGVGDVAYFGPVISVSRTVTVDSPRTVGYLGFEGNQQYTIAGNSI
                     TMDSQVSNTIEVVSGVHQVTAPMFLNKDTLINITGANDSLRLEHVAASGGVTISKMGA
                     GALNIAPVGQQANLAIAAGSVNMISSAAVGATSFNNVDVASGTKWNLISYDQTVNTIA
                     NNGTIDLGFGRLTVAPGDAGSSFAGTITGSAGVLNAGLSIAGVVNPNVSGSGILTLSG
                     NTTYQGDTNILSGGLKYVAGNIAATASAFTVATGASLDLTNNSQTIGSLAGAGSVALG
                     SATLSLGGNNLSTAFSGTISGAGGITKTGSGTFVLGNANPFSGTTTINAGVVQLTHAL
                     ALQNSTVQLNVNNGLDLNGLAATVGTISGGGNLAISGPSLTVGGNGNTTVYSGSLSGG
                     GQLIKTGAGSLILRGGNSYTGGTLINGGGTLNINDDSNLGQGGAGVTMNNALLYWNTS
                     SAWTTSRPIAVTGGTGALQTDNATVTLNGVISGPGTFVKTGDGTIVLNATNTVTGGMN
                     LQAGQLELDNNSALGTGTLAATNITPGANIVISKTSGAPVTTINNPIRLDLGATSIDF
                     RVDNGFTLDLEGPISGTSDFQKGTGGASGANVGTGILALGGNNSAYNGTITVQTGTLS
                     VRSNTALGAPGGGTRVSPGATLSFDGALNYAAAEPIALAGTLAATGGATTFGGSITLA
                     GPSTVNIAAGSSFTTGNITGAFTLTKTGSGTLTLSGPNAYTGDTVINGGSVNVLAGAS
                     VATQHVTVTAGASFTTAGSLSNVQTLVITDASTNVQSTGSMTVGGPSSPVAISFLGNN
                     NPSIQLFAGATSGGHLTILSDMAFTGSAGTAGIFAAGSGAVPGALDLGTGVRTISVAD
                     GSAPIDLEISAPVINGGINKSGPGTLRLSGADGYAGGTTITGGTIEASTPSALGAGVV
                     TFGSGGAGLRLVSDAPTLTLANALTTAADNSISVDIEPINNPA"
BASE COUNT          495 a          982 c         1133 g          608 t
ORIGIN      
        1 gcggggttgt tgatcggctc gatatcgacg ctgattgagt tgtcggcggc cgtcgtcaag
       61 gcgttcgcca gcgtaagcgt cggcgcatcg ctgacgagcc gaagtccagc gccgccgctg
      121 ccaaaggtga cgacgccggc gccgagcgcg gacggtgttg acgcttcaat cgtgccgcca
      181 gtgatggtgg ttccaccggc gtagccgtcg gcgccgctga gccgaagcgt gcccgggccg
      241 ctcttgttga tgccaccatt gattacgggg gcagaaatct ccaggtcgat cggcgctgaa
      301 ccgtcggcga cgctgatcgt gcgcacgccc gtgcccaggt cgagcgcgcc aggcacggcc
      361 ccgctgccgg cagcgaagat gccggcggtg ccggccgatc cggtgaaggc catgtccgac
      421 aagatcgtca ggtgcccgcc gctggtcgcg ccggcgaaga gctggatcga cgggttgttg
      481 ttgcccagga acgagatggc gactggcgac gacggcccgc ccacggtcat cgaaccggtg
      541 gattgcacgt tggtgctggc gtcggtgatg accagcgtct gcacgttcga gagggatccc
      601 gccgtggtga atgacgcgcc ggcggtgacg gtgacgtgct gcgttgcgac cgatgcgcca
      661 gccaacacgt tcacgctgcc gccgttgatg acggtgtcgc cggtatacgc gttggggccg
      721 ctcaacgtga gcgtgccgga acccgtcttg gtcaacgtga acgcgcccgt gatgttaccg
      781 gtggtgaagc tggagcccgc ggcgatgttg acggtggatg ggccggccag cgtgatgctg
      841 ccgccgaagg tcgtcgcacc gccggtggcg gcgagcgtgc cggccaaggc gatgggctcg
      901 gccgcggcat agttcagcgc gccgtcgaac gacagcgtcg cgccgggcga cacgcgcgtg
      961 ccgccgccgg gggcacccaa tgcggtgttg gaccgcaccg acagcgtgcc ggtctgcacg
     1021 gtgatggtgc cgttgtaggc tgagttgttc ccaccgagcg ccaggatacc tgtgccgacg
     1081 ttcgcccctg acgcgccgcc ggtgcctttc tggaagtcgc tcgtgccgga aatcgggcct
     1141 tccaggtcca gtgtgaaccc gttgtccacg cggaagtcga tgctggtcgc gcccaagtcc
     1201 aggcggatgg gattgttgat cgtggtgacg ggcgcgccgg acgttttgct aatgacgatg
     1261 ttcgcgccgg gcgtgatgtt cgtcgccgcc agcgtgccgg tgccgagcgc actgttattg
     1321 tccaattcca actggcccgc ctggaggttc atgccgccag tgaccgtatt ggttgcgttt
     1381 aatacgatcg tgccgtcgcc ggttttgacg aacgtgccgg ggccggagat gacgccgttc
     1441 agcgtgacgg tcgcgttgtc ggtttggagc gcgccagtgc cgccggtgac ggcgatcggg
     1501 cggctggtgg tccacgcgct gctggtgttc cagtagagca gcgcgttgtt catcgtgacg
     1561 ccggcgccgc cctggcccaa gttggaatcg tcgttgatgt tgagcgtgcc gccgccgttg
     1621 atcagcgtgc caccggtgta ggagtttcca ccgcgaagga tcagggaacc ggcgccggtc
     1681 ttgatcagct gaccaccacc gctgagcgag ccactgtaga cagtggtgtt gccgttgcca
     1741 ccgacggtga gcgatgggcc ggagatggcg aggtttccac cgccggagat cgtgccgact
     1801 gtggccgcca gtccattcag atccagaccg ttgttcacgt tgagctgcac cgtcgagttc
     1861 tgcagcgcca gcgcgtgggt cagctggacc acgccggcgt tgatcgtcgt cgttcccgag
     1921 aaggggtttg cattgccgag aacgaacgtg cccgacccgg tcttggtgat gccgccggcg
     1981 cccgagatcg ttccggagaa cgcggtagag agattgtttc caccgagcga aagcgtggcg
     2041 ctgccgagcg cgacgctgcc agcgccggcg agggaaccga tcgtctgcga gttgttcgtc
     2101 agatccagcg atgccccggt ggcgaccgtg aacgccgacg ccgtcgccgc aatgttcccc
     2161 gcgacgtact tcaatccgcc agacaggatg ttcgtgtcgc cctggtaagt cgtgttgccg
     2221 gacagcgtga gaatgccgga gccgctgacg ttggggttga cgacgccggc gattgagagc
     2281 cccgcgttga ggacgcccgc cgagccggtg atcgtgccgg cgaaactgct gccggcgtcg
     2341 cccggcgcga ccgtcaatct accgaagccc aggtcgatcg tgccgttgtt ggcaatcgtg
     2401 ttgacggtct ggtcgtagct gatcaggttc cacttcgtgc ccgaggccac atcgacgttg
     2461 ttgaacgagg tggcgcccac cgcggcggag ctgatcatgt tcacgctgcc ggcggcgatc
     2521 gccaggttcg cctgctggcc gaccggtgcg atgttgagcg cgcccgctcc catcttgctg
     2581 atcgtgacgc cgccgctggc ggcgacgtgc tccagccgca ggctgtcgtt ggcgccggtg
     2641 atgttgatga gcgtgtcttt gttcaggaac atcggggccg tcacctggtg cacgcctgag
     2701 acgacctcga tcgtgttgct cacctggctg tccatcgtga tggaattgcc ggcgatcgtg
     2761 tactgctgat tcccttcgaa gccgaggtaa ccgacagtcc tgggcgaatc gacggtgacg
     2821 gtgcgagaaa cgctgatgac cggtccgaaa tacgccacgt cgcccacgcc gttcgggctc
     2881 gcgacgttcc agttgctcgc gtcggaccag ttgccgtcgg catccacctt ccaggccgtg
     2941 ccgatatccg ccattgattt ggagaggaat ccaccgtcgt acgcgacgcg gttttggcct
     3001 gcggggaaga gcgagttcaa gttgatgaag gcgttgccag aaatggaata ggcgtcgagg
     3061 tggttcagga cgagcgtcga gccattgagg atgcgcgcgc cgtggccgga aacgtcgagt
     3121 aggtaaagcg cctctttgcc gctgccgccg cgatggccgt tgtcgatggc gtccgtcagc
     3181 tcaaccaccg cgcgctggct cgccgaaccg atgaccag
//
LOCUS       DATIMH010001018         1547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4285270, whole genome shotgun sequence.
ACCESSION   DATIMH010001018 DATIMH010000000
VERSION     DATIMH010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4285270"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..977)
                     /locus_tag="VLI90_17740"
     CDS             complement(219..977)
                     /locus_tag="VLI90_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16108.1"
                     /translation="MRFAIAPLLLPLPALMLSLLVGCAGNGATSPPQSGAAALIVDVK
                     ANPKAGYRPANYHVLVYDAPTPATPADFEKVDYAALDEVVVWVEPTNAAGATSGDNAD
                     LLPARLDVDPQKASDHLAAAVSVGQRLIFRNTGDKPGAVYSVSDGNNFHLGTLAPGAR
                     GEYTVQSPGLIEVVTTSLADPIATVYAAPTRWVRLTRAGQRIYFNDLPPGRYKIVSWH
                     PRLPGHETTVNLPSDRVMRASIAVGVNGLPKIDQ"
     gene            complement(974..>1547)
                     /locus_tag="VLI90_17745"
     CDS             complement(974..>1547)
                     /locus_tag="VLI90_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16109.1"
                     /translation="HAAEPPTTQPTGVITGTVSAPGEVPLSEMVVFLIPDDNQSITPP
                     KDVATISQKDAHFAPSLLIVCVGQSVNFLNDEDRLIEHNVFSNSPTKQFDLGLYKPGE
                     GRTVTFDKPGSVNLYCSIHRHMDGVIYVAPTPYFSRVAADGSYKIENVPPGHWVVATW
                     QRRRRFQEVRAGAKVSADQPVVQDLSLKAK"
BASE COUNT          268 a          494 c          497 g          288 t
ORIGIN      
        1 cgtgatcagc agcatgagca ggagaatctt ggtgccgatc cgcatgaaaa gactttggta
       61 cagcggcgga acacgcgcgt agcgattcac ctgcgtgggg attcgtctgc gtcgcgattc
      121 ctgcgtagcg gtcgccccgc aggggcacgt tcttttcgtt cgggcgccaa aacacgtgcc
      181 cctgcggggc taccgcaacg caggtggctc ggcgcacgtc attggtcaat cttcggcagt
      241 ccgttcacac cgacggcgat cgaagcgcgc atcacgcgat cgcttggcag gttcaccgtt
      301 gtctcatgcc ccggcaggcg cgggtgccag gagacgatct tgtatcgccc cggcggcagg
      361 tcgttgaaat agatgcgctg gccggcgcgc gtcaggcgca cccagcgcgt cggcgcggca
      421 tagacggtgg cgatcggatc cgccagcgac gtcgtcacca cctcgatcaa cccgggtgat
      481 tgcacggtat attcgccgcg tgcgcccgga gcgagcgtgc cgagatgaaa gttgttgccg
      541 tccgaaacgc tgtagaccgc gccgggtttg tcgcctgtgt tgcgaaagat cagccgttgg
      601 cccaccgaca ccgcggcggc gagatgatcg gacgctttct gcggatcgac atccaaccgc
      661 gccggcagca agtccgcgtt gtcgccggac gtcgcgccgg ccgcgttggt tggctcaacc
      721 cacacgacga cttcatcgag cgccgcgtaa tccaccttct caaaatctgc cggcgttgcc
      781 ggcgtcgggg cgtcgtagac cagcacgtga tagttcgccg ggcgataacc ggccttgggg
      841 tttgctttca catccacgat cagtgctgcc gcgcctgatt gcggtggtga tgtagcgccg
      901 ttaccggcgc agccgaccag cagcgagagc atcaacgccg gcagcggcag cagcagcggg
      961 gcgatcgcga atctcatttg gccttcagtg aaagatcctg aaccaccggt tgatcggctg
     1021 acacttttgc gcccgcgcgg acttcctgaa agcgccggcg gcgttgccag gtcgccacca
     1081 cccaatgccc gggcgggacg ttctcgatct tgtaactgcc gtcggccgcc acccggctga
     1141 agtacggcgt cggcgccacg taaatcacgc cgtccatgtg ccggtggatc gagcaataca
     1201 gattgaccga cccgggcttg tcgaacgtga ccgtgcggcc ttcgcccggc ttgtatagcc
     1261 ccaggtcgaa ctgcttggtt ggcgaattcg aaaacacgtt gtgctcgatc agccggtctt
     1321 catcgttcag gaaattcacg ctctgcccga cgcagacgat caacagcgac ggcgcgaaat
     1381 gcgcgtcctt ctgggagatc gtcgccacgt ccttcggcgg cgtgatcgac tggttgtcat
     1441 cgggaatcaa gaagaccacc atctccgaca gcggcacttc gccgggggcg ctgaccgtgc
     1501 cggtgatgac gcccgtcggc tgcgtcgtcg gcggctcggc ggcatgc
//
LOCUS       DATIMH010001019         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_531900, whole genome shotgun sequence.
ACCESSION   DATIMH010001019 DATIMH010000000
VERSION     DATIMH010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531900"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /locus_tag="VLI90_17750"
     CDS             <1..1907
                     /locus_tag="VLI90_17750"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSV16110.1"
                     /translation="RYETDRANDEISLAINRYLRHLDEHYQQVNRVVQVMLGDPTMRS
                     LLAATDDSGDASAAEQLRAAKTQLKEEVFGRDVQAELTTVAGPPAFHVLINFAHDVVL
                     VALPRHLATTTTAPAGDEQLERALASRAVHWPVDAVIDAKPRPVMQYIATPGGLFLAM
                     GIPLREELSQPPTHAYFAGFRIDDDWIRQQMLAQQASGASSVAPLSAWFVLDNDIVAR
                     ASSDPNDKRVAAFTAATAMSRAAETTPTTGSSVVHVAFNLAGEHFVGQSFDLNPQDAR
                     TGRLVLASSLDRALGPLHYLQRSILLVTGVTIVLAMIACEALALMISRPINQLVAGTK
                     RIAAGQFDLPVNVRRRDELGTLADSFNEMSQGLKQRDGLLEERVKIQRDMAVARKIQM
                     DVLPKQLPVCRGYDMAAYSLPAEQTGGDIYDLVAVALDSEKASDNASLVLLLADATGH
                     GIGPALSVTQVRAMLRIGVRLHAQLDEVFAQMNRQLCQDLGSERFVTAFLGLLDPNNH
                     SINYQSAGQGPLLHFHSRTREMEWLVPSMLPLGIDEDPVSEGSRQMELEPGDLIVLLT
                     DGFYEFQNPERELFGRDRVAKIIMENHQLTARELMEELISATREFARGAPQLDDMTCV
                     IIRRLNAEVA"
     gene            2078..>3112
                     /locus_tag="VLI90_17755"
     CDS             2078..>3112
                     /locus_tag="VLI90_17755"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HSV16111.1"
                     /translation="MRLAFVTLLIALPLLPAAAHAQDYAASGPYSIVRWDEDYSYLKD
                     PAKRTDFFDPVKYIPIGDSPDAYISFGGQIRDRFDYFNNNEFAGGPRDTGFNLIRLLA
                     HADVHFNSHIRGFLQLISALEDGRAGGPRTGDADDFDIQQAFVDLSFAFGDNADYGSS
                     IVLRVGRQELIYGAQRLLSPNDWRNVRQSFDGVKASVYLPNDTLDVFLTRPVLINKTH
                     LNSSDDHTYFGGIYNVTELPRVLPEAHSKLDLYLLALNKIKSATTAVDSNTYTLGARF
                     HTTPLPWDFDFEPDWQFGTTAGNQIAAWAIAGEAGYTFQSVPLTPRLAIGADIASGSV
                     DAAHRFNQLFP"
BASE COUNT          574 a         1036 c          931 g          571 t
ORIGIN      
        1 cgcgctatga gactgatcgc gccaacgatg agatctcgct ggccatcaac cgctaccttc
       61 ggcacctgga cgagcattac cagcaggtca accgggtcgt gcaggtgatg ctgggcgatc
      121 ccacgatgcg gtcgctgctg gccgccaccg acgacagcgg cgatgcatca gcggccgagc
      181 aactccgcgc cgccaagacg caattgaagg aagaagtctt cggccgggac gtgcaggccg
      241 agctgaccac ggtcgccggc ccgccggcgt ttcacgtgct gatcaacttc gcgcacgatg
      301 tcgtgctcgt cgcgctgccg cggcatctgg cgacaaccac gacagcgccc gcgggtgacg
      361 aacaactcga acgcgcgctg gcatcgcgcg cggtgcactg gccggtcgat gccgtgatcg
      421 acgccaaacc ccggccggtc atgcaataca tcgccacgcc cggtggattg ttcctggcga
      481 tgggcatccc gctgcgcgaa gagctgagcc agccgcccac gcacgcgtac ttcgccggct
      541 tccgcatcga cgacgactgg atccgccagc agatgctggc ccagcaggcc agcggcgcat
      601 catccgtcgc cccgctgtcg gcgtggttcg tgctcgataa cgacatcgtc gccagagcat
      661 cgtccgatcc aaacgacaag cgcgtcgcgg cattcaccgc cgccacggcg atgtcgcgcg
      721 ctgccgagac gacgccgacg accggatcgt ccgtcgttca cgtcgcgttc aatctggccg
      781 gagaacattt cgttggccag tcgttcgatc tcaatccgca ggatgccaga accggccggc
      841 tggtgctggc cagctcattg gatcgcgcgc tcggcccgct gcattacctg cagcgatcga
      901 tcctgctcgt cactggcgtc accatcgtgc tggcgatgat cgcctgcgaa gcgctggcgc
      961 tgatgatctc ccgcccgatc aaccagctgg tggccggcac caagcgcatt gccgcggggc
     1021 aattcgattt gccggtcaac gttcgccggc gcgacgagct gggcacgctg gccgacagct
     1081 ttaacgagat gtcgcagggc ctgaagcagc gcgacgggct gctcgaagag cgcgtgaaga
     1141 tccagcgcga catggcggtg gcgcgcaaga tccagatgga cgtgctgccc aagcagctcc
     1201 ccgtctgccg cgggtacgac atggccgcgt attccctgcc ggccgagcaa accggtggcg
     1261 atatttacga tctcgtcgcc gtcgcgctcg attcggaaaa ggcgagtgac aacgcgtcgc
     1321 tcgtcctgct gctggccgac gccaccggtc acggcatcgg cccggcactg tcggtcacgc
     1381 aagtgcgcgc gatgctgcgc atcggcgtgc gcctgcacgc gcagctggat gaagtctttg
     1441 cgcagatgaa ccgccagctc tgccaggacc tgggttccga gcggttcgtc accgcattcc
     1501 ttggcctgct cgatccaaac aaccacagca tcaactatca atccgccggc caggggccgc
     1561 tgctgcattt ccattcgcgc acgcgcgaga tggaatggct cgtcccctcg atgctgccgc
     1621 tcggcatcga cgaagacccg gtcagcgaag gttcgcgtca aatggaactt gaaccgggcg
     1681 acctgatcgt cctgctgacc gacggcttct atgaattcca aaaccccgag cgcgagctat
     1741 tcggccgcga ccgcgtggcg aagatcatta tggaaaacca tcagctgacc gcacgcgagt
     1801 tgatggaaga gctgatctcc gcgaccaggg agttcgcccg cggcgccccg cagctggatg
     1861 acatgacctg cgtcatcatc cgccggctga atgcggaggt ggcctagcgg tggcctagcg
     1921 gtgcctcatc accaaccacg ccgggttcgg aagtaccccg acgacccgca tccttgtctg
     1981 gcaaaaggtt gatgatcttc aattcgcctg tcacgatcgt atcggaaaag ttcggctggc
     2041 cttgcgacag gttaccatgc cgagcacgag gaatttcatg cggctcgcgt ttgtaacgct
     2101 cttgatcgcc ctgccgttgc ttccggccgc cgctcatgcg caggattacg ccgcttcggg
     2161 cccgtactcc atcgtccgct gggatgagga ctattcctac ttaaaggatc cggccaagcg
     2221 cactgatttc ttcgatccgg tgaagtacat tccgatcggt gacagccccg atgcgtatat
     2281 ctccttcggc ggccaaatcc gcgaccgctt cgactatttc aacaacaacg aatttgccgg
     2341 tggtccgcgc gataccggct tcaacctcat ccgcctgctc gctcacgccg acgtccattt
     2401 caattcgcac atccgcgggt ttctgcagtt gatcagcgcg ttggaagacg gccgcgccgg
     2461 tggcccgcgc acgggcgacg cggatgattt cgacattcag caggcgttcg tggatctgag
     2521 ctttgcgttc ggcgacaacg ccgattacgg cagctcgatc gtccttcgcg tgggccgcca
     2581 ggaactgatc tatggggcac agcgcctcct cagccccaac gactggcgca acgtccgcca
     2641 gagctttgac ggcgttaaag cgtcggtcta tttgcccaac gacacgctcg atgtgttcct
     2701 cacccgcccg gtcctcatca acaaaacgca cctgaacagc agcgacgatc acacgtattt
     2761 cggcggcatc tacaacgtga ccgagttgcc gcgcgtgctg cccgaggcgc actcgaagct
     2821 ggacctgtac ctgctggcgc tgaacaagat taagtcggcg acgacggcgg tggattcgaa
     2881 cacgtacacg ctcggggcgc gctttcacac aacgccgctg ccttgggatt tcgatttcga
     2941 acccgattgg caattcggca cgaccgcggg aaatcagatc gccgcctggg cgatcgcagg
     3001 tgaagcggga tacacgtttc aaagcgtgcc gctcactccg cgcctggcga ttggcgcgga
     3061 catcgcctcc ggttcagtcg atgccgcgca tcgattcaac cagctgttcc cg
//
LOCUS       DATIMH010001020         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5445429, whole genome shotgun sequence.
ACCESSION   DATIMH010001020 DATIMH010000000
VERSION     DATIMH010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5445429"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VLI90_17760"
     CDS             <1..364
                     /locus_tag="VLI90_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16112.1"
                     /translation="ASGGTRTTPVDFATLVKKNPSAVAGKGSRCFGAQVGARAKTVVV
                     KIERLQRSVQSSPSKSSPSQRHRAKRLEPRARPTKQAHFFLELFFVVFLEDFLAAFFA
                     MALNHLLSVIDEFTGREI"
     gene            complement(469..1470)
                     /locus_tag="VLI90_17765"
     CDS             complement(469..1470)
                     /locus_tag="VLI90_17765"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16113.1"
                     /translation="MIITAHAHARAGLVGNPSDGYFGKTISFIIRNFSATVRLWESPH
                     FEILPTHGDLARFDNINSFLRDVRMNGYYGGLRLMKAAVKKFHDFARAQGRELHAKNF
                     TLSFDSDIPRLVGLSGSSAIIVATLRALSKFYDYEISQHLLPQLILSVESEELGIAAG
                     LQDRVIQTYEGIVYMDFDRQLMESRGYGEYTPLKPAKLPPLYVAYDPDRAEISDIPHR
                     NLRELYRRGDATVVGAMQKYRELTDRGRAALMAGDWAALGQVMNANFDLRRTIMPIAP
                     ENLRMVEVARATGASAKFAGSGGAIVGLYRDGRQYQQLTDALAAIRCAILRPLIFES"
     gene            complement(1591..3495)
                     /locus_tag="VLI90_17770"
     CDS             complement(1591..3495)
                     /locus_tag="VLI90_17770"
                     /inference="COORDINATES: protein motif:HMM:NF042614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HSV16114.1"
                     /translation="MPDSPTVLSQPPDRTPLTAAVIRARMSNEWRGLRNRPAIDWLAD
                     AFVILFGVIATMTVCGYEFAHGDHTVYLVEPLHLAHPELLRNDWFTTQTLQYHSAFSG
                     ITSILMRLNIVSQGFLIGYLALVFIFQMAWLTIVRTVGGSRTAYLLSIILYFMSRGGV
                     VLASYSILQDSELLASNIAAVMLLVGIALWLRRWIIPAGLAFGMAGMFHLNYAVAAIG
                     GWCGLVALSYLIKTTPRLSGRALLVGTLFMLAGAFPNIIHALPGALKQRHAGMPLAQF
                     IELYVRIRHPHHYDPSRWSGLEWLTFCWPIPFAVFVLSTWTRRGDVQPEVIDARRQLA
                     RVYIGFAIALLIAFIGAGIWYLSTTLVQLSLYRFGVYVHLIGCIAAALLVCDGIARPV
                     LPTARIRRLMVFGTGVSIVIAAMLIVAFGTTDPTNGLESFRRYFAPALIALTCLAFVP
                     AVYAIVRAYAPLVWQRRIFAVVSVLLLALLAIRRGTWRGMVYVGDHTIDQMITCQWIR
                     DPKHTPLDAIFLVSPGEQIFRLHAQRAIVVNFKSPPQLTTELPQWRDRLQDVLGMPSL
                     APLADFRGDMMGHLDELYDAVPDQTILAAARKYGAEYILLSHRLPGQFDSALVYSREN
                     GTYFLYHIPL"
     gene            complement(3513..3722)
                     /locus_tag="VLI90_17775"
     CDS             complement(3513..3722)
                     /locus_tag="VLI90_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16115.1"
                     /translation="MSEDAIRQLMLLRLQLRVEPEMTRYIHRRLTAGPTGALPVIGSD
                     ARTGIAIRKIITAGDFAMSSSPPPQ"
BASE COUNT          732 a         1181 c         1241 g          661 t
ORIGIN      
        1 tgcgtcaggt ggaacgcgaa ctactccagt cgactttgcc accctagtaa aaaagaaccc
       61 ttccgccgtg gcagggaagg gttctcggtg tttcggggcc caagttggag ccagggccaa
      121 aacggttgtg gtgaagatcg agcgtctaca acgcagtgtt cagtcgtcgc cgagcaagtc
      181 ctcaccgagc cagagacacc gggcgaaacg tttggagcca cgcgcccggc ctacgaagca
      241 agctcacttc ttcttggagc tcttcttcgt cgtcttcttg gaagacttct tagcggcttt
      301 ctttgccatg gcgctcaatc acctcctttc tgttatcgac gaatttacgg ggcgcgaaat
      361 ttagagtcaa tccttttttg tgattgcgaa ttcttttttt gcgataaatg atttcgcgcg
      421 cgctgcgcgc gaatcaagtt cgagtgcgcg caggcgcgcg cgcgcggctc aactttcgaa
      481 gattaacggg cgaagaatcg cgcagcggat ggccgcgagg gcgtcggtga gttgctggta
      541 ttgccgacca tctcgataca agccgacgat cgcgccaccg ctgccggcga acttggcgct
      601 ggcgccagtc gcgcgggcca cctccaccat ccgcaggttt tccggcgcga tcggcatgat
      661 cgtccggcgg agatcgaagt tcgcgttcat cacctgcccc agcgcggccc aatcgccggc
      721 catcagcgcc gcgcgcccgc gatcggtcag ctcgcggtat ttctgcatcg cgcccacgac
      781 ggtggcgtcg ccccggcgat acagctcgcg caggttgcgg tggggaatgt cgctgatttc
      841 cgcacgatcc gggtcatacg ccacgtacag cggcggcaac ttcgccggtt tcagcggtgt
      901 gtattcgccg tacccgcgcg attccatcag ctgccgatcg aaatccatgt acacgatgcc
      961 ttcataggtc tggatcaccc ggtcctgcag gccggccgcg atgcccaact cttcgctttc
     1021 gaccgacagg atcaactgcg gcagcaggtg ctgggaaatc tcgtagtcgt agaacttcga
     1081 cagcgcgcgc agcgtggcga cgatgatcgc gctgctgccg gacaggccga cgaggcgggg
     1141 gatatcgctg tcgaagctca gcgtgaagtt cttggcatgc agttcccgcc cctgcgcgcg
     1201 ggcgaagtcg tggaacttct tcaccgcggc cttcatcagg cgaaggccgc cgtaatagcc
     1261 gttcatccgc acgtcgcgca ggaagctgtt gatgttgtcg aagcgggcca ggtcgccgtg
     1321 ggtgggcagg atctcaaagt gcgggctttc ccacaggcgc acggtggcgc tgaagttgcg
     1381 gatgatgaag ctgatggttt tgccgaaata cccgtcggat ggattgccga ccagcccggc
     1441 ccgggcgtgc gcgtgtgcgg tgatgatcat gttgctctct cgccgcgcgc gatccggctg
     1501 cggcagagcc gctgtagggg cgacgcctgc gtcgcccttg tcaccggagg agattcgcca
     1561 acggcgaatc gggcgacgca ggcgtcgccc ctacagcggg atatgataaa gaaagtacgt
     1621 gccgttttca cgggaataga cgagcgccga atcgaattgg ccggggaggc gatggctcag
     1681 caggatgtat tcggccccgt atttccgcgc cgccgccagg atcgtctgat ccggcaccgc
     1741 gtcatacagc tcgtccagat ggcccatcat gtcgccgcgg aagtccgcca gcggcgccag
     1801 gctgggcatc cccagcacat cctgcaaccg atcgcgccac tgcggcaact ccgtcgtcaa
     1861 ctgcggcggc gacttgaagt tcacgacaat cgcccgctgc gcgtgcaggc ggaagatctg
     1921 ttcgcccggc gataccagga agatcgcatc aaggggcgtg tgtttcgggt ccctgatcca
     1981 ctggcaggtg atcatctggt cgatcgtgtg atcgccgacg tacaccatcc cgcgccaggt
     2041 gccccggcga atcgccagca gcgccagcag cagcacgctc accaccgcga agatgcgccg
     2101 ctgccagacg agcggcgcgt aagcgcgcac gatcgcatag acggcgggga cgaaggcgag
     2161 gcatgtcagc gcgatcagcg ccggggcgaa ataccggcgg aaggattcca ggccgttggt
     2221 tggatcagtc gtgccgaatg cgacgatgag catcgccgcg atgacgatcg acacgccggt
     2281 gccaaagacc ataagccgcc ggatgcgcgc agtgggcaac acgggccggg cgatgccgtc
     2341 gcagacgagc aacgccgcgg cgatgcagcc gatcagatgc acgtacacgc caaagcgata
     2401 gaggcttaat tgcaccagtg ttgtgctgag gtaccagatg ccggcgccga tgaacgcgat
     2461 cagcagcgcg atggcgaagc cgatgtagac gcgcgccagt tgccggcgcg catcaatcac
     2521 ctctggctgc acatcgccgc ggcgcgtcca ggtggatagc acgaacaccg caaacggaat
     2581 cggccagcag aacgtcaacc attccaaacc gctccaccgc gacgggtcgt aatgatgcgg
     2641 gtggcggatg cgcacataca gctcgatgaa ctgcgccagc ggcatgcccg cgtggcgctg
     2701 tttgagcgcg cccggcaacg cgtgaatgat gttcggaaac gccccggcca gcatgaacag
     2761 cgtgccgacg agcagcgccc gcccgctcaa ccgcggggtg gtcttgatca ggtacgacag
     2821 cgccacaagc ccgcaccacc cgccgatggc ggcgaccgcg tagttcaggt ggaacatccc
     2881 cgccatccca aacgccaacc ccgccgggat gatccatcgt cgcagccaca gcgcgatgcc
     2941 aaccagcagc atcacggcgg cgatgttcga cgcgagcaac tcgctgtcct gcaggatgct
     3001 gtacgacgcc agcaccacgc cgccgcggga catgaagtag aggatgatcg acagcaggta
     3061 cgccgtgcgc gatccaccga cggtccgcac gatggtgagc cacgccatct ggaagatgaa
     3121 caccagcgcg agatagccga tgagaaaccc ctggctgacg atgttcagcc gcatcaggat
     3181 gctggtgatg ccgctgaacg cggagtggta ttgcagcgtc tgggtggtga accagtcgtt
     3241 gcggagcagc tcggggtgcg ccaggtgcag cggctcgacg aggtagaccg tgtgatcgcc
     3301 gtgggcgaat tcgtacccgc agacggtcat cgtcgcgatg acgccgaaca ggatgacgaa
     3361 ggcgtcggcg agccagtcga tggcgggtcg gttgcgcagg ccgcgccatt cgttcgacat
     3421 gcgcgcacga atcaccgccg ccgtcagcgg cgttcgatca ggaggctgcg ataacaccgt
     3481 tggcgagtca ggcacgttgg ttaatcgaaa agttactgcg ggggcgggct tgaggacatc
     3541 gcgaaatcgc ccgcggtgat gattttcctg atcgcgatgc ccgtgcgggc gtcgctgccg
     3601 atgaccggca gcgccccggt cggtccggcg gtgagccgcc gatggatgta gcgggtcatc
     3661 tccggctcga cgcggagctg caggcgaagc agcattagct ggcggatggc gtcctcactc
     3721 acgcgcgcgg gtgctccctg gatcgacgac ggcgacgaca gcggtttgaa tgatatcgcg
     3781 tggatcagcc gcgccgctta aagaattgca ccgcc
//
LOCUS       DATIMH010001021         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5731024, whole genome shotgun sequence.
ACCESSION   DATIMH010001021 DATIMH010000000
VERSION     DATIMH010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5731024"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VLI90_17780"
     CDS             complement(<1..324)
                     /locus_tag="VLI90_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16116.1"
                     /translation="MKKLIALSVMMTVAGCAPAHPESAAGVAPEQRAWMDRSLPPERR
                     AQLLVGQMTLDEKILQIHMVDQKDRPREVAPVPRLGIPTFRITNGPAGAGPGDAKPTQ
                     PATALP"
     gene            439..1533
                     /locus_tag="VLI90_17785"
     CDS             439..1533
                     /locus_tag="VLI90_17785"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSV16117.1"
                     /translation="MLLHIYRIRGAILDTSLARITFINLLMMLGASRTVAQTAPSSAP
                     AADGSIISTKPGEAVDASHDDYVRYLQREYEDDVKSAAELGVTLPSIDQMRAGWPTAS
                     EYQHRLHDPRFECDRLLYRSDGLKVVGYLFKPVNPPRDQRLPAIIFNRGGNADVAQVG
                     TITLLEIYPFLEAGFVVLAPQYRGSDGGEGHDEYGGADVHDVLNVITLARSLDYVDPD
                     NLFMYGESRGGMETYLAIKQHAPINAAAVLGASANEVEENHRRPLTDVYEKAVPNFDR
                     DPGAAFRARSAALWAEAIDKPILILHGGADWRVAPGQALEMATRLEQLHKPYQLFIYA
                     GDDHHLTLHHTERDRQVLDWFRRFMKPASK"
     gene            complement(1511..1990)
                     /locus_tag="VLI90_17790"
     CDS             complement(1511..1990)
                     /locus_tag="VLI90_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16118.1"
                     /translation="MSQALPSSAGMPMREPMDLQHLHDPTYQAYQFLHVGLTVAPIFA
                     GLDKFFHLLVNWDNYLAPVVNNILGGHGHQFMLAVGVIEIVAGIGVALWPRIFSYVVA
                     VWLLGIIINLLLIPASNTMPHYDIALRDLGLCLAALALGRLSTVVVHARATTSTPAS"
BASE COUNT          541 a          824 c          841 g          493 t
ORIGIN      
        1 cggcaacgcc gttgccggct gtgtcggctt cgcgtcaccc ggccccgcgc ccgcagggcc
       61 gttggtgatg cgaaacgtcg gaatgcccag ccgcggcacc ggcgcaactt cgcgcgggcg
      121 atccttctga tcgaccatgt ggatctgcag gatcttctcg tcgagcgtca tctgccccac
      181 gagcaattgt gcccgccgct ccggtggcag cgagcggtcc atccacgccc gctgttccgg
      241 cgcgaccccg gcggccgatt ccgggtgagc cggagcgcac ccggctacgg tcatcatcac
      301 gctcaatgcg atcagctttt tcacgggcac gaattcctcc ggtgatggat atggctagac
      361 tgcaatgtga ttgttacaca gacaatggca gaactgttga tgggccaact catccaattc
      421 acggctatca tccgtcacat gctcttgcac atctatcgca tccgcggcgc gatcttggac
      481 acgtcccttg cgcgcatcac attcatcaac ctgctcatga tgctcggcgc ttcgcggacc
      541 gttgcccaga cagcgccatc gtccgcgccg gctgccgatg gttccatcat cagcaccaaa
      601 cccggcgaag ccgtcgatgc gtcgcatgat gattacgtgc gataccttca gcgcgagtat
      661 gaagacgacg tgaagtccgc cgcggaattg ggcgtgacgt tgccgtcaat cgatcagatg
      721 cgggccggct ggccgacggc gagcgaatat cagcatcgcc tgcacgaccc gcgattcgag
      781 tgcgatcggc tgctctatcg cagcgatggg ttgaaggtgg tcggctatct gttcaagccc
      841 gtcaatccgc cgcgcgatca gcggctgccg gcgatcatct tcaatcgcgg gggcaacgcc
      901 gatgtcgcgc aggtggggac gatcacgctg ctggaaatct atccgttcct ggaggctggg
      961 ttcgtcgtgc tagccccgca ataccgcggc agcgacggcg gtgagggcca cgatgaatac
     1021 ggcggggcgg acgtacacga tgtgctgaac gtcatcacgt tggcgcggtc gctcgactac
     1081 gtcgatccgg acaatctctt catgtacggc gaatcccgcg gtgggatgga aacatacttg
     1141 gccatcaagc agcacgcccc cattaacgcc gctgcggtgc tgggcgcgtc ggcaaatgaa
     1201 gtcgaggaga accatcgccg gccgctgacc gatgtctacg aaaaggccgt gccgaatttc
     1261 gatcgtgatc cgggcgcggc gtttcgcgca cgctccgccg cgctatgggc agaggcgatc
     1321 gacaaaccca tcctgatcct gcacggcggc gccgactggc gcgtcgcccc gggccaggca
     1381 ttagaaatgg cgacgcggct cgagcagttg cataagccgt atcagctgtt catctacgcc
     1441 ggagacgatc atcacctgac gctgcatcac acagagcgcg atcgacaggt gctcgactgg
     1501 ttcaggcggt tcatgaagcc ggcgtcgaag tagtcgcccg tgcatgcacg accacggtgc
     1561 tgagccggcc cagcgccagc gctgccaggc acaatccaag gtcgcgcagc gcgatgtcgt
     1621 aatgcggcat cgtgttcgat gcggggatca gcagcaggtt gatgatgatg cccagcaacc
     1681 agaccgcgac gacgtagctg aaaatcctcg gccacagcgc gacaccgatg cccgcgacga
     1741 tttcaatcac accgacggcg agcatgaact gatgcccatg gccgccgagg atgttgttga
     1801 ccaccggcgc cagatagttg tcccagttga ccagcagatg gaagaacttg tcgagcccgg
     1861 cgaagatcgg cgcaacggtc aatccgacgt gcaagaactg gtacgcctga tatgtcggat
     1921 cgtgcagatg ctgcaggtcc atcggctccc gcatcggcat gccggcggat gacggtaatg
     1981 cttgagacat aaaaacctcc ggatagttga aatatccggg gtacgtctcg caaatcggac
     2041 gccgaattac gcgggagaag cgctcgaatt gatttaaaat tagcgagccc ggcggttgcc
     2101 acacgtttta attggaatgc cgacatcgac cgccggcgcg gcagcagtac cgtcgcgccc
     2161 agcaacagcg acagggctgt cggttccggc accgtcgcgg gctgaaacga gatgttccca
     2221 aagccgtccg ccatcgtgat ggggatgatc ttcacgtaag acatcggcgt gacgccggaa
     2281 ctgaaggtga tgtcatcttc gtaaatgccc ggcgtgtgga acgtgtcggt tcgtgaggcg
     2341 acgaggttcc cgccgttgtc gtaaccttcc aggcggagcg acccgccgac ggtttggtcc
     2401 gcgccgtacg ccatctccat gttcagctgc gccaccggca cggggaacat catcgtgaac
     2461 gcgaattcat acgagcgaga agaagcttcc cgcagagcgc ggcggggcgg tcaagacatt
     2521 gcccggcgtc atgttcggca ggtagccgac cccaacgcac cggctggatg gcaaacccgt
     2581 tcccgtcggc ggatgtggga ttgacaaagg taattcccgc cgtcggatcg gtgaacgacg
     2641 ccatagccgt gttcaccggg agggaattga atgaagcgat aatggcggcg gtggctgag
//
LOCUS       DATIMH010001022         5522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1110178, whole genome shotgun sequence.
ACCESSION   DATIMH010001022 DATIMH010000000
VERSION     DATIMH010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110178"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..559)
                     /locus_tag="VLI90_17795"
     CDS             complement(65..559)
                     /locus_tag="VLI90_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16119.1"
                     /translation="MKAMTYTGPDGSVVPRPDEELLERIIRQERGRYWRVGSGGSGLA
                     VIELKGKDKRTRQAIDGEPALAFFLVEPHGFFFTFFEPGKFVEQLVPFAGGTSRPWVE
                     HVVCGLEMYVPRACFVPRNTAWEIIQEFTRTKQRSRAVTWVNRSTLDFPSPEERRLSK
                     ADLA"
     gene            complement(664..1083)
                     /locus_tag="VLI90_17800"
     CDS             complement(664..1083)
                     /locus_tag="VLI90_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16120.1"
                     /translation="MRYPLNANKQVSCPVCKKPGPGTKSPVTPRPEAVSLGFTVLSHV
                     HGRSYPVFLGSMLMLGWDSGVSKHPQSRSSIGAAFIAAKKDGRDAEVGQMELHFCSTR
                     CLRRFFKDAIDELDRRIAAERPALQKARKSRRPKAAA"
     gene            complement(1315..1839)
                     /locus_tag="VLI90_17805"
     CDS             complement(1315..1839)
                     /locus_tag="VLI90_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16121.1"
                     /translation="MKRRLFNVLAAVSLVLCVATAAACILSYQRLDAIGSFEDHSPIV
                     AQAKGRLLIGFGSLLKPNEHHRVERSNDVQIGTLAWGYVFGDMLETAHGFAGFWFAIS
                     RPLGAQDKRNWCLIVPHWACFVATAILPLTAYAGWRRTARWQRKGRCPRCGYDLRATP
                     QRCPECGTAVKPAA"
     gene            complement(1946..5335)
                     /locus_tag="VLI90_17810"
     CDS             complement(1946..5335)
                     /locus_tag="VLI90_17810"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HSV16122.1"
                     /translation="MVKRVTGANFLFAESHKRRHPRLFLPKYLADEAEHFTVDAGELQ
                     HAAAALVRWADAIEQGQLKQKETSLDAEFLQVIFGEALGYKSISDSPADYHREKNPTV
                     PGIGTADGALGYFTAGKDNPPAVVIELKGADTDLDHDKFNGRTPVQQLWDYLNYLPDT
                     QWGIVSNYATIRLYHKARTARAYEEFTVADFKDPVRLKQFYFIFERHGLIGTKVKEGR
                     GRATNLLNRTQTQQREIGDKLYAYYAEQRELLIAELMQHKQFTQDEAIHAAQRLLDRI
                     IFMAFCEDRGLLPEKLIETTWKHVAPLARATNPRWRNFLDAFVAIDKGHPSLDLPTGY
                     NGGLFREDPLVDTLQLDDRWADVFKNIGNYDFREEGEINVDVLGHIFERSITELEKAR
                     VVGLFGAQAAAALAAMPKSAERKRFGIYYTPPQFTQLIVEETLGKLITERVESIPDVR
                     RRVDALRQLKVVDPACGSGAFLIAAYERLEDAYEDVAREMRIAGHLQDALNLSNAYAD
                     YILHDNLYGVDLSGEAVEITQLALWIRSARKGRALTDLSKNIRRGNSLVRDPNVHPQA
                     FDWRTEFADVFKRDVEGFDCVIGNPPWERMKVQEREFFSLAAPAIANAVNAADRRKLI
                     AAVETANPKLWERYTVAQQAAERTLSYVRSVAANYPLTGKGDVNTYMLFAELARQIVS
                     PTGKVGLLVPSGIATDNTTKDFFADLMESKTLAALYDFENRKLIFPDVDGRFKFSVLL
                     MNGAKVQHENASFVFFAHAIEDLTDAKRQIDLSPKDLKLVNPNTRTCPIFRNTRDAEL
                     TRQVYRRIPVLIDFSRKSGGNPWGVQFCRMFDQTNDAEKFVEGATLKADGGKLSGNRW
                     TNNARVYLPLYEAKMIQMYDHRAAGVRIEAGNWMRQGQTEDTTLVEHQNPEFVVQPRY
                     WVDSMLVEDIAGKRPALLAYKDVTSATNQRTMIAAMVPEVALMNSAPLILTGSVETGT
                     KLTPRRELCLLANLNSLAYDFIARQKVGGLHLNFFIVEQLPTLPPDAYDERCPWAKSR
                     KLEDWIAERVLKLTCTADDMRPLAVAAGFAEGVHKWKEPERAQLRAELDAAYFILYGI
                     DRGDVEYILSTFQGVVKEDEAHGGAGVTRSLILEAFDSMRP"
BASE COUNT          985 a         1739 c         1717 g         1081 t
ORIGIN      
        1 cccgccggct cggtccactg catcggcggg ttatgtcgct ggccaaattg ccgtccgcac
       61 gcaatcacgc aaggtccgcc ttcgacagcc ggcgttcctc cggactcggg aagtccagcg
      121 tcgaacggtt cacccacgtg accgcgcggg accgctgttt cgttcgcgtg aattcttgaa
      181 tgatctccca cgccgtgttc cgcggcacga agcaggctcg cgggacgtac atctctaagc
      241 cgcagaccac gtgctcgacc cacggacggg acgtgccgcc ggcgaacggc accaactgct
      301 cgacgaactt ccccggctcg aagaaggtga agaagaatcc gtgcggctcg acaagaaaga
      361 atgccaacgc gggctcgcca tcgatcgcct gccgcgtcct cttgtcctta cctttgagct
      421 caatcaccgc aaggccggag ccgccgctgc cgacccgcca gtagcgaccg cgctcctggc
      481 ggatgatccg ttcgagcaac tcctcatccg gacgcgggac cacgctgccg tccggaccgg
      541 tgtacgtcat cgccttcaca ggtgctccag caacataacg tagtgccgat caacggtgga
      601 gccggactcc aaccgcgctc gaattaaaga aacaataccg cggctgcggg ccgttgcatc
      661 gggttatgca gccgctttgg gtcggcggga cttccttgcc ttctgcagcg ccggtctctc
      721 cgcggcgata cgccgatcca actcgtcaat ggcgtccttg aagaaccggc gcaggcatcg
      781 cgtcgagcag aagtgcagtt ccatctgccc gacttccgcg tctcgaccgt ccttcttcgc
      841 ggcgataaat gccgcgccaa tcgatgagcg gctctgcgga tgcttagaga cgccggagtc
      901 ccatccgagc atcagcatcg aaccgagaaa caccggataa ctgcgtccgt gaacgtgcga
      961 caaaacggtg aacccgagcg acaccgcttc gggccttggc gtgactggtg acttcgtgcc
     1021 cggtcccggc tttttgcaga cgggacagga aacttgcttg ttggcgttga gggggtatcg
     1081 catagggtgc cgcacggccc ggggctgcct aacgtattgc cgttcatcgg tcgggcctca
     1141 gcgccgcgtc gagcgtcaac gccgctcgcg gcacgattcc gatgcagcgg ttggttctgt
     1201 tgcacgtcgc gccgccacca tggaagtacg aggcactgaa ccggcggccg agggtacgtt
     1261 gctggcgcga cgtccgctgc aacgtacccg ttctgcgcgc ggcgtgcgag gggcttacgc
     1321 cgccggcttg acggccgtgc cgcattccgg acagcgctgc ggcgtggccc gcaggtcgta
     1381 gccgcagcgg gggcaccggc ctttccgctg ccatcgagcg gttcgccgcc atccggcata
     1441 agccgtaagg ggaagtatcg ccgttgcgac gaaacatgcc cagtgaggga cgatcaagca
     1501 ccagttccgt ttgtcctggg cacccaaggg gcgcgagata gcgaaccaga acccggcgaa
     1561 cccgtgcgcg gtttccagca tgtcaccaaa cacgtatccc caggccagcg tgccgatctg
     1621 cacgtcattt gatctctcaa cccgatggtg ctcattcggc ttcagcaacg agccaaagcc
     1681 gatgagcaat cgccccttcg cctgcgcaac gatgggcgaa tggtcctcaa aggagccgat
     1741 cgcatccaac cgctggtagc tcaggatgca agcggctgcc gtcgcgacgc acagcaccag
     1801 cgacaccgcg gccagcacgt tgaacaggcg acgcttcacg gcggacatcc taccgcgcgg
     1861 cgggagatga gcgggacggc tctgaatcag tcagaaaatg gaacgcaagt tattttgcga
     1921 gtctttgcat cccgatctga tggttctacg ggcgcatgct gtcgaacgcc tccaggatca
     1981 gcgaccgcgt cacgcccgcg ccgccgtgcg cttcgtcttc cttcaccacg ccttggaagg
     2041 ttgacaggat atattcgaca tcgccgcggt cgatgccgta gagaatgaag tacgccgcgt
     2101 ccagctccgc ccgcagctgg gcgcgctcgg gctccttcca cttgtggacc ccttcggcaa
     2161 accccgccgc caccgccagc ggccgcatgt cgtccgccgt gcaggtcagc ttcaacaccc
     2221 gctcggcgat ccagtcttcc agcttcctgg acttggccca cggacaccgc tcgtcatacg
     2281 cgtccggcgg caacgtgggc agctgctcga cgatgaagaa gttcagatgc agcccgccca
     2341 ctttctgccg tgcgatgaag tcgtaggcca gcgaattgag atttgccaac aggcatagtt
     2401 cccgccgtgg cgtgagcttc gtcccggttt cgacggaacc cgtcagtatc agcggcgccg
     2461 aattcatcag cgccacttcc ggcaccatgg ccgcgatcat ggttcgctgg ttcgtcgcgc
     2521 tggtaacatc cttatatgcc agcagcgccg gtcgctttcc cgctatgtct tcgaccaaca
     2581 tcgaatcgac ccaataccgc ggctggacaa cgaactccgg attctgatgc tccacgagcg
     2641 tggtgtcctc ggtttgcccc tggcgcatcc agttacccgc ttcgatgcgc acgccggcgg
     2701 cgcggtggtc gtacatctgg atcatcttcg cttcgtacag cggcaggtaa acgcgcgcgt
     2761 tgttggtcca gcggttccca ctcaacttgc cgccgtcggc tttcaatgtt gcgccttcaa
     2821 cgaacttctc cgcatcgttc gtttgatcaa acatgcggca gaactgcacg ccccacgggt
     2881 tgcccccgga ttttctgctg aaatcaatga gaactggaat gcggcgatag acctgcctcg
     2941 tcagctcggc atcgcgcgtg ttgcggaaga tcgggcacgt gcgcgtgttc ggatttacca
     3001 gcttcaggtc cttcggcgat agatcgatct gacgcttggc gtcggtgagg tcttcaatgg
     3061 catgcgcgaa gaagacgaag ctggcattct cgtgctgcac cttcgcgccg ttcatcagca
     3121 gcacgctgaa tttgaaccgg ccgtccacat cggggaaaat cagcttgcga ttttcaaagt
     3181 catacaacgc ggcgagggtc ttgctctcca tcaggtcggc gaagaagtcc ttggtcgtat
     3241 tgtcggtggc gatgccgctg ggaacgagca ggccgacttt tccggtgggg gagacgatct
     3301 gccgcgccag ctcggcgaag agcatgtacg tattcacgtc gccttttccg gtgagcggat
     3361 aattggccgc tacggaccgg acgtagctca gcgtgcgctc cgccgcctgc tgtgccaccg
     3421 tgtaccgttc ccacagtttc ggatttgcgg tttccacagc cgcgatgagc ttgcgtcgat
     3481 cggccgcgtt caccgcgttg gcgatcgccg gcgcggcgag cgagaagaat tcccgctcct
     3541 gcaccttcat ccgctcccac ggcgggttgc cgatcacgca atcgaatccc tcgacgtcgc
     3601 gcttgaagac atcggcaaat tcggtccgcc aatcaaacgc ctgcgggtgg acgttcggat
     3661 cgcgcaccag gctgttgccg cggcggatgt ttttggacag gtccgtcagc gcccggccct
     3721 tgcgggccga gcggatccac agcgccagtt gcgtgatctc gacggcttcg ccggacaggt
     3781 ccacgccgta caggttgtca tgcaggatgt agtcggcgta ggcgttgctc aggttcaggg
     3841 catcctgcag atgtcccgcg atgcgcatct cgcgcgcgac gtcttcatac gcatcttcga
     3901 gccgttcgta agcggcgatg aggaacgcgc ccgagccgca ggcgggatcg accaccttca
     3961 gctggcgcag cgcatcgacg cgccggcgga catcggggat cgattcgacg cgttcggtga
     4021 tcagcttgcc gagtgtttct tccacgatca gctgcgtgaa ctgcggcggg gtgtagtaga
     4081 tgccgaagcg cttgcgctcg gcgcttttgg gcatggccgc caatgctgcg gccgcctgtg
     4141 cgccgaatag accgaccacg cgggcctttt ccaattcggt gatcgagcgc tcgaagatgt
     4201 gaccgagcac gtcgacgttg atttcgcctt cctcgcggaa gtcgtagttg ccgatgtttt
     4261 tgaacacgtc ggcccagcgg tcgtccagct gcagcgtgtc gaccagcggg tcttcgcgga
     4321 acaggccgcc gttgtagccg gtcggcagat caagcgaggg atggccttta tcgatggcga
     4381 cgaaggcgtc gaggaagttg cgccagcgcg ggttggtggc ccgcgccagc ggcgcgacgt
     4441 gcttccacgt cgtctcgatc agcttttccg gcagcagccc gcggtcttcg cagaaggcca
     4501 tgaagatgat gcggtccagc agccgctggg cggcgtgaat tgcctcgtcc tgcgtgaact
     4561 gcttgtgctg catcaattcg gcaatgagca actcgcgctg ctcggcgtaa taggcgtaaa
     4621 gcttgtcgcc aatctcacgc tgctgcgtct gcgtgcggtt gagtagattg gtggcgcggc
     4681 cgcgaccctc cttcaccttg gtgccgatca gaccgtggcg ttcgaagatg aaataaaact
     4741 gcttgagccg aaccgggtct ttgaagtcgg cgacggtgaa ttcttcgtaa gcgcgggcgg
     4801 tgcgcgcctt gtggtaaagc cggatcgtcg cgtagttgct gacgatgccc cactgcgtat
     4861 cgggcaggta gttcagataa tcccacagct gttgaaccgg cgtgcggccg ttgaacttgt
     4921 cgtgatcgag atcggtgtcg gcgcctttga gctcgatgac gaccgccggc gggttgtcct
     4981 tgccggcggt gaagtaaccc agcgcgccgt cggcggtgcc gatgccgggg acggtcgggt
     5041 tcttttcgcg gtgataatcc gccggtgagt cggagatgga cttgtagccc agcgcttcgc
     5101 cgaagatcac ctgaaggaac tcggcgtcga gcgacgtttc cttttgcttg agctggccct
     5161 gctcgatggc gtcggcccag cgcaccagtg cggcggcggc gtgctgcagt tcgccggcgt
     5221 cgaccgtgaa atgctcggct tcgtcggcca gatatttcgg caggaacagc cgcgggtggc
     5281 ggcgtttgtg ggactcggcg aataaaaaat tggcgcccgt gacacgtttg accatcgcga
     5341 cgccaagcgt atcggaacgc catcaattcg caatcacgat gcggcgccgg tggcctcggg
     5401 gtgtagcgct cacttgtggc tcagcaagtc acatgagcgc cagccagcgc ctacgccaag
     5461 aacgccaaga aaagaaataa cagattgaac cgcagagacg ccgaggcgcg gagaggtgcc
     5521 cg
//
LOCUS       DATIMH010001023         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_822899, whole genome shotgun sequence.
ACCESSION   DATIMH010001023 DATIMH010000000
VERSION     DATIMH010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822899"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1646)
                     /gene="ffh"
                     /locus_tag="VLI90_17815"
     CDS             complement(174..1646)
                     /gene="ffh"
                     /locus_tag="VLI90_17815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HSV16123.1"
                     /translation="MFDALTDRFNSVFRSLSGKGRISEENVRESMREIRTALLEADVN
                     FKVVNEFTEKVIQKAIGQEVIKSLQPGQLMVKIVYDELLELLGPVDTNIYFVQPGPTV
                     IMMCGLQGSGKTTTSGKLAKYLVAKGHHPLLAAVDLQRPAAVEQLRVLGEQVGVPVYT
                     DNSKIAPHGQVAKGAAVAVARAAVAQAKSSGSDVVILDTAGRLAIDQELMDELKDINT
                     QINPQQIYLVLDSMTGQDAVESARRFNEQLELDGLILTKLDSDTRGGALLSAKMVTGK
                     PVKFLGMGEKLEALDEFRPEGMAQRILGMGDIVGLVSEAMQKFDAEETAKLQAKMEKG
                     NFTLDDFMAQMNQVRKLGPMGKVMGMIPGMSELTKQIGQNEGAVESQMNHMKAIYESM
                     TRDERKKPELLDGGRRRRIARGAGVELNEVGQFMKQFEASRDMMRAVGGMGMGGKMKM
                     MKALMGGGLANMGVPGGPMLKTKRSGWMAPKDRNKKKKRR"
     gene            1752..2183
                     /locus_tag="VLI90_17820"
     CDS             1752..2183
                     /locus_tag="VLI90_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16124.1"
                     /translation="MHQPPDHDPSVADVQAAFRRHGVEPPALFCAIVKRRMIGFAAFT
                     AFEPWQLCGLEEIVPLSQRWPTARLPQDLIPFARHQGSDDLACFQLQHEDAATSITGI
                     REVHYDLGPPVYVELGAEFDDFWQWLHSALDDVRFSIEHGG"
     gene            complement(2212..2661)
                     /locus_tag="VLI90_17825"
     CDS             complement(2212..2661)
                     /locus_tag="VLI90_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16125.1"
                     /translation="MPAAAGYCPRCGASLAPSSRELWQARAVAIRQQSLRWAAAIIRL
                     RPRARHNIYAGEPNRSKIITGYGNAMFNLGWRYERRHNPPEAIRCYFKSSKLGNADAA
                     TRLAPAEEAAKEQPTPADPPVPAFRLWRDWDAARATIQRRADDQERE"
     gene            complement(2950..>3591)
                     /locus_tag="VLI90_17830"
     CDS             complement(2950..>3591)
                     /locus_tag="VLI90_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16126.1"
                     /translation="LSGTGDSVAHMAHIGGTIFGTSVCLVLLIAQLLPRDQFDVWALI
                     QRWNKRRQYRDLVSKGFNPFEPVPPARGKRALPDPVMERVHEIRGQIGDALTNRDNAA
                     AAKLYLQLKAVDPAQVLSRQGQLDVATQLHHEGLYEQAAEAYESLLKTYPTVERSEQV
                     ELMVGLIYARYLNKFEQAKHHLSKAVARLHGGRELELAQEELSRVELQLGAAR"
BASE COUNT          592 a         1168 c         1092 g          739 t
ORIGIN      
        1 gtttcttctc gcacgaagct cgaagacaga ttgaaccacg aagctcacga agagcacgaa
       61 acggaggaaa aacttggact ttctgcttcg tgctcttctt gagcttcgtg gttcaaatta
      121 ttgttccgga aacacgtgcc ccttcggggc taccgctacg cgggggatat cgttcaccgc
      181 cgcttttttt tcttattgcg gtccttgggt gccatccacc cggagcgctt ggtcttcaac
      241 atcggcccgc cgggcacgcc catgttggcc aacccgccgc ccatgagggc tttcatcatc
      301 ttcatcttcc cgcccatgcc catgccgcca acggctcgca tcatgtcgcg gctggcttcg
      361 aactgcttca tgaactggcc gacttcgttt aattccacgc cggcgccgcg ggcgattctc
      421 cgccgccggc cgccgtccaa cagctccggc ttcttgcgct cgtcgcgcgt catcgactcg
      481 tagatggcct tcatgtggtt catctgcgat tcgaccgcgc cttcgttctg gccgatctgt
      541 ttggtcagct cgctcatgcc tgggatcatg cccatgacct tgcccatggg gccgagcttg
      601 cgcacctggt tcatctgggc cataaagtcg tcgagcgtga agttgccctt ctccatcttg
      661 gcctggagct tggcggtttc ttccgcgtcg aacttctgca tcgcctcgga gaccaggccg
      721 acgatgtcgc ccatgccgag gatgcgctgc gccatcccct cgggacggaa ctcgtccagc
      781 gcctcgagct tctcgcccat gcccaggaac ttcaccggct tgccggtaac catcttcgcg
      841 ctcagcagcg cgccaccgcg ggtgtcgctg tccagcttcg tcaggatcag gccgtccagt
      901 tcgagctgct cgttgaagcg tcgggcggat tcgacggcgt cctggccggt catgctgtcc
      961 agcaccaggt agatctgctg cgggttgatc tgcgtgttga tgtccttcag ctcgtccatc
     1021 aactcctggt cgatggcaag ccgaccggcg gtgtcgagga tgacgacatc gctgccactc
     1081 gatttcgcct gcgcgacggc cgcgcgcgcg acggccaccg cggcgccttt ggcgacttgc
     1141 ccatgcggcg cgatcttgct gttgtcggtg taaacgggca caccgacttg ctcgccgagc
     1201 acgcgaagct gctcgacggc ggcaggacgc tgcaggtcca ccgcggccag cagcgggtga
     1261 tgtcccttgg cgaccaggta ttttgcaagc ttgccgctgg tcgtcgtttt gccgctgccc
     1321 tgcaggccgc acatcatgat gacggtcggc ccgggctgga cgaaataaat gttcgtatcg
     1381 acggggccga gcagttccag cagctcgtcg tacacgatct tcaccatcag ctggcccggc
     1441 tgaaggctct tgatgacttc ctggccgatc gccttctgaa tgaccttctc ggtgaattcg
     1501 ttgacgactt taaaattgac gtccgcttcc agcagcgcgg tgcgaatctc gcgcatggat
     1561 tcgcggacgt tctcttcact gatgcggccc ttgccgctga ggctgcggaa gacggaattg
     1621 aagcggtcgg ttaatgcgtc gaacatgcag gcaattctag ccgctgtgcg gcgcggttgc
     1681 accggtctgg gtatatccta agcgcagcgg cgcagccgcg cagtcgaagg atctggtgat
     1741 aaggttatga catgcaccaa ccacccgacc acgatccgtc cgtagccgac gttcaagccg
     1801 cgttccgccg acatggcgtt gaaccgccgg cgctattctg cgcgatcgtc aagcgtcgca
     1861 tgatcggctt cgcggcgttc accgcgttcg agccgtggca gctgtgcgga ttggaagaaa
     1921 tcgtgccgct gtcgcagcgc tggcccacgg cgcgcttgcc gcaggacctg atcccgttcg
     1981 cgcgccatca aggcagcgat gatttggcat gttttcaact acagcacgag gacgccgcaa
     2041 catccattac cggcattcgc gaggtgcatt acgacctcgg cccgcccgtg tacgtcgaac
     2101 tcggcgcgga attcgacgac ttctggcagt ggctccacag cgcgttggac gacgtgcgct
     2161 tctcgatcga acatggcggc tgaatgcggt ggccagagcg atggcggcgc atcattcccg
     2221 ctcttggtcg tccgcgcgcc gctgaatggt cgcgcgagcc gcgtcccagt ctcgccacaa
     2281 gcgaaacgcc gggaccggcg ggtcggcggg cgttggctgt tccttggcgg cctcctcggc
     2341 cggcgccaac ctcgtcgccg cgtcggcgtt gccgagcttc gatgacttga aataacaacg
     2401 gatcgcctcg ggcgggttgt gccggcgttc gtagcgccag ccgaggttga acatcgcatt
     2461 gccgtagccg gtgatgatct tcgagcgatt cggctcgccg gcgtagatgt tgtgacgggc
     2521 acgcggacgc aggcgaatga tcgccgccgc ccagcgcagg ctctgctgtc gaatcgcgac
     2581 tgcacgcgct tgccacaact cgcgtgacga cggcgccagc gacgcgccgc aacgcgggca
     2641 gtaccccgct gccgctggca aatgtccgac cgccgccccg caatggcggc actcagatcg
     2701 cgcggagata tgcgggtcga tggacatcaa atcgacgcgt taggttcaca cgccaattgt
     2761 atcgcgtaaa tgagcgaagg gtgaggtgga agttttcgtg cgaagcggaa cgcctcaatc
     2821 gtgacacggg cctccggctc gtgcaaaacg cgcgcgaacg tctgggaatc cgggcgttgc
     2881 tactccaagc gcatctcaca cgggcccgag gtccgcgtca caattggcgt ccgcgacgat
     2941 gcccgcgtat taccttgccg cgcccagctg caactccacg cgcgatagtt cttcctgcgc
     3001 caactcaagt tctctgccgc cgtgtaatct ggcgaccgct ttggacaaat gatgcttcgc
     3061 ctgctcgaac ttgttcagat accgcgcgta aatcagccca accatcagct ccacctgctc
     3121 cgagcgctcg accgtcgggt aggttttcag cagcgattcg tacgcttcgg ccgcctgctc
     3181 gtacaagccc tcgtgatgca gctgtgtcgc gacgtccagc tgcccctgcc gcgacaacac
     3241 ttgagcggga tcgaccgctt tcagctgcag gtaaagcttc gccgcagcgg cgttgtcgcg
     3301 attggtcagc gcatcgccga tctgcccgcg aatctcatgc acgcgctcca tcaccggatc
     3361 gggcagcgcg cgcttgccgc gcgcgggcgg cacgggttcg aacgggttga agcccttgct
     3421 gaccaggtcg cgatattgcc ggcgcttgtt ccagcgctgg atcagcgccc agacgtcgaa
     3481 ttgatcgcgc ggcagcagct gggcgatcag cagcacgagg cacacgctgg tgccgaagat
     3541 cgtcccgccg atgtgggcca tgtgggcgac gctgtcgccg gtgccgctga g
//
LOCUS       DATIMH010001024         3828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_823086, whole genome shotgun sequence.
ACCESSION   DATIMH010001024 DATIMH010000000
VERSION     DATIMH010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823086"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2998)
                     /locus_tag="VLI90_17835"
     CDS             complement(<1..2998)
                     /locus_tag="VLI90_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSV16127.1"
                     /translation="MLEDRKLLSASGIGAFAPTTATWSLRSTPTAGPADIGTFQFGAY
                     VPVTGDWNGDGVDDIGTFNPATATWSLRYETSAGPADAGVFVFGKKRTLPVVGDWNGD
                     GRDDIGVYDPKTAQWSLRLGASAGPANAGTFTFGSKNALPVAGDWDGDKKDGIGTFSP
                     PSSTWTLRQTPNGGSANVGKFWYGPKNTLPAVGDWNGDGKDGIGTVDPKTATWRLRQT
                     PNTGTPNAGTFVFGPKYALPVSGIFNAPAPAANVISNITIKPLDLNLLGLEVQTSPIT
                     ITVSTTAGDAKLIGNMLSSGQNLINTTQVNGALNNVLNTATDLVNTSPLSLLGVSSGA
                     FDTAAPSTTQVMELFVAPTHLATLGAQIDTSPIRLSISAHAGNGLVLGNALTVLSNLF
                     NPPLPSSLDIDFINQKLADTLDQLNLQLGSIPAADVPAIQPNPGDILTFAMPGIDMNL
                     LGLNLKTDPVLVDAATQANDGSLLGNVWAVPLQSSPATPDKMAQMVNTADGVLARVFG
                     ALDASSLTLASSAITGLSPATQALLSPTLFGPAGSSAQIVQLTSSASANSPATVSLLG
                     LSIASNNLNASLSATTGDGLVLGNLLYNASNLTNPGASASMVSLLSQLASGSTAAVAP
                     ISGVPAPATSSSSQLFTVTLPPLDLNSLGLEVQTDAVTVKLTAESGGSDLLGNVLRGY
                     TSLLNLSGISAAVNNVLSAATGLANSASLSVAGVGSGAFDTASPSTTQVLDATVAPIH
                     FDVAGMLAETNPIHLKLIAHAGNGLVLGNVLTTLANLFNAPLPDHVDTAAINGKLQQL
                     LTNLDAQAPGIPPATLPSAAPDPATVAEVTVPALNLDLLGMTLKTDPIFVGVAAHTGN
                     GDLLGNVYTTLLNTLTSSSADLGGVSNNVNALLAKVIGVFNASTLTLSASALSSLAPA
                     YQTLASPTLINPTAGATASIIDLGNAATGTNLPSSATMLGLAGLTSNIHLTLTARTGD
                     GLVLGNMLYNLANLLNGGTSPGVLYLL"
     gene            3291..3782
                     /locus_tag="VLI90_17840"
     CDS             3291..3782
                     /locus_tag="VLI90_17840"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HSV16128.1"
                     /translation="MAEEQPSLHIDTDWKKQAQEEKRRLAEQAAKNKPPATPPEAPAT
                     SPSSAGGAMPVDPRAAAAGSPAGGATRRGRGQRELPAPGFASLVQSVMTQVLYYLGDL
                     ASSAGAMGVDLDMAKYQLDQLTMLEDKTKGNLSDDECRLLDAALYETRSRFIGVASQF
                     LGP"
BASE COUNT          680 a         1175 c         1312 g          661 t
ORIGIN      
        1 gcaggagata cagaacgcct ggcgaggtgc ccccgttgag caggttcgcg aggttgtaga
       61 gcatgttgcc caagaccagt ccgtcgccgg tgcgggcggt caacgtgaga tggatgttgc
      121 ttgtgagacc ggccaggcca agcatggtgg cgctgctcgg gagattcgtg ccggtggcgg
      181 cgttgcccag gtcaatgata gacgccgtcg cgccggctgt tgggttgatc agcgtcgggc
      241 ttgccagcgt ctgatatgcc ggggccagcg aagagagcgc gctggcggag agggtcagcg
      301 tggaggcgtt gaacacgccg atcactttgg ccagcagcgc gttgacgttg ttgctcacac
      361 cgcccaagtc tgccgatgag ctggtcaacg tgttcaacag cgtcgtgtag acgttgccca
      421 gcagatcgcc gttgccggtg tgggcggcga cgccgacgaa gatcgggtcg gtcttgagcg
      481 tcatgcccag cagatcgaga ttgagagcag gcaccgtaac ctctgccacg gtcgccgggt
      541 ccggcgcggc cgatggcaac gtcgccggcg gaatgccggg tgcctgcgcg tccaggttcg
      601 tcagcagctg ctgcaacttc ccgttgattg ccgcggtgtc cacgtgatcc ggcaacggag
      661 cgttgaacag gttggccaac gtcgtgagca cgttgcccag caccagcccg ttgccggcgt
      721 gggcgatcaa cttcaggtgg atcgggttcg tctccgccag catgccggcg acgtcaaagt
      781 gaatcggcgc gacagtggcg tccagcacct gcgtcgtaga tggcgacgcg gtgtcgaacg
      841 cgcccgagcc gacgccggca acggacaggc tggcggaatt cgccagaccg gttgcggccg
      901 acagcacgtt gttcaccgca gcgctgatgc cactcaggtt cagcaggctc gtgtacccgc
      961 gcagcacgtt ccccagcaga tcgctgccgc cggattccgc ggtcagcttc accgtgacgg
     1021 cgtcagtctg cacttccagg ccgagcgagt tcagatcgag cggtggcagc gtgacggtga
     1081 acaactgaga cgaactcgat gttgcgggcg cgggcacgcc actgatcggc gcaactgccg
     1141 cggtgctgcc ggatgccagc tgcgacagca gcgagaccat cgatgccgat gcgccggggt
     1201 tggtgaggtt cgacgcgttg tacagcaggt tgccgagcac cagcccgtcg ccggtcgttg
     1261 ccgacagcga cgcgttgaga ttgttgctcg cgatgctcag gccgagcagg ctcacggttg
     1321 ccggggaatt tgcggatgcg gatgacgtca actgaacgat ctgcgccgac gagcccgccg
     1381 gcccgaacag cgtcggcgac agcagcgcct gcgtcgccgg cgagagtccg gtgatcgcgc
     1441 tggacgccag cgtcagcgat gatgcgtcga gcgcgccaaa cacgcgcgcc agtacgccgt
     1501 cggccgtgtt gaccatctgg gccatcttgt ccggcgtcgc ggggctcgac tgcagcggaa
     1561 cggcccacac gttgcccagc agcgatccgt cgttcgcctg cgtggcggca tcgaccagca
     1621 ccggatcggt cttcaggttc agaccgagca ggttcatgtc gatcccgggc atggcaaagg
     1681 tcagaatgtc accgggattc ggctggattg ccggcacatc cgccgcggga atgctgccga
     1741 gttgcaggtt gagttggtcc agcgtgtcgg ccagcttctg gttgatgaag tcgatgtcga
     1801 gcgacgacgg cagcgggggg ttgaacaggt tcgacaggac cgtcagcgcg ttgcccagca
     1861 ccagcccgtt gccggcatgc gcggagatgg acaggcggat ggggctcgtg tcgatctgag
     1921 cgcccagtgt cgccaggtgc gtcggcgcga cgaacaactc catcacctgc gtggtcgaag
     1981 gcgcggccgt gtcgaacgcg cccgatgaca cgccgaggag cgaaagcggt gatgtgttca
     2041 ccagatcggt cgccgtgttc agcacgttgt tcagcgcgcc gttgacctgc gtggtgttaa
     2101 tcagattttg cccgctgctg agcatgttgc cgatcagctt ggcatcgccg gccgtcgtgc
     2161 tgacggtgat cgtgatcggg cttgtttgca cctcaaggcc gagaagattg aggtcgagcg
     2221 gcttgatcgt gatgttcgaa atcacattgg cggcaggcgc cggcgcgttg aagatccctg
     2281 agaccgggag cgcgtatttc ggcccgaaca caaacgtgcc ggcgttgggt gtgccggtgt
     2341 tgggcgtctg ccgcaaccgc cacgtcgccg tcttgggatc gacggtgccg atgccgtcct
     2401 tgccgtcacc gttccaatcg cccacggcgg gaagtgtatt cttcggccca taccaaaact
     2461 tcccgacgtt ggcggagccg ccgttgggtg tttgccgcag cgtccaagtg gaactgggcg
     2521 gtgagaacgt gccgatgcca tccttcttat cgccatccca atccccggcg acgggcaatg
     2581 cgttcttgct gccgaatgtg aaggtgccgg cgttggctgg acctgccgac gcgcccaggc
     2641 gcagcgacca ctgcgccgtc ttcgggtcat acacgccgat gtcatcgcgg ccgtcgccgt
     2701 tccaatcgcc gaccaccggc agcgttctct tcttgccgaa cacgaacacg cccgcatcgg
     2761 ccggccccgc ggacgtctcg taacgcagag accacgtcgc ggtggcaggg ttgaaagtgc
     2821 cgatgtcgtc cacgccgtcg ccgttccagt caccggtgac cggcacgtac gcgccgaact
     2881 ggaaggtacc gatgtcggcc ggccccgcgg tcggcgtcga gcgcagcgac cacgtcgcgg
     2941 tggtcggcgc aaacgcgccg atgccggacg ccgacagcag ctttcgatct tccaacactt
     3001 caaaggcaaa aggtgagcca gcgccagagc gggtctgagc gtgagcacga gaagtctttc
     3061 gccgcattga tttccctcca aggatgtgag cgctgatacg aaacccgcgg cgatgctaga
     3121 cagcggtggt ggcccgcgcc aaggcaaatc cgatgcaaga ttttgcggaa aaacatcgac
     3181 gcatgccgga tatgccggac gtacactcgg tgcattctcg ccggtgtcgg cggcggagca
     3241 cgcttgcaaa agcgtcccgc tggtcgagaa tcgaatcagg agcgacgccc atggctgaag
     3301 aacaaccctc gttacacatc gacacggact ggaagaagca agcgcaggaa gagaagcgcc
     3361 ggctggcgga gcaggcggcg aagaacaaac ccccggccac gccacccgag gcgccggcga
     3421 cgtcaccgtc atctgccggg ggcgcgatgc cggtcgatcc gcgcgctgcg gccgcagggt
     3481 cacccgctgg cggggcgact cgtcgcgggc gcggccagcg cgagctgccg gcgccgggct
     3541 tcgcgtcgct cgtgcaatcg gtcatgacgc aggtgctgta ttacctgggc gacctggcca
     3601 gctcggccgg ggcaatgggc gtcgatctgg atatggccaa gtatcagctc gatcagctga
     3661 cgatgctgga agacaagacc aagggcaatc tgagcgacga cgaatgccgg ctactcgacg
     3721 cggcactgta cgaaacgcgc tcgcggttca tcggggtggc gtcgcagttc ctcggcccct
     3781 gagaaatgaa ccacgagatc ggcgcgctcg attaatagcc ggattgaa
//
LOCUS       DATIMH010001025         6562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_533024, whole genome shotgun sequence.
ACCESSION   DATIMH010001025 DATIMH010000000
VERSION     DATIMH010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533024"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1317
                     /locus_tag="VLI90_17845"
     CDS             124..1317
                     /locus_tag="VLI90_17845"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HSV16129.1"
                     /translation="MSQTDDQTKTPESPQTPKRKRRLIWLILRALGWCIIGLILIALI
                     ARPFMPWAVRWYVNRTLDQSILYRGKIGELNLHLWRGAYTIHDVRISKTTGNIPVPLF
                     STKELDLSVQWNALLHGRVVGQVKMLQPEVNFVDDPDPANSETGAGGPWLKMLRDLFP
                     FDLNRAELHDGAIHFRTYQKATPADVYLSHVEARIDDLQNVNRSLAPLLATVSATATA
                     MDQGKLDFKMRLDPFSYNPTFHLALRLLGLDVTKTNPVLETYGGFNVKSGFFDLVIDM
                     DSNEGQLSGYVKPLFRDMVIFDLVKDVKQDKDALQVFWQALVGGAAAIVTNYNRNQLG
                     TLIPFTGELTTPGVDYLAAIGNVLRNAFIRAYLPRLEKTRQTEDNLTFGPPSLADPIS
                     VENQQ"
     gene            1339..2016
                     /locus_tag="VLI90_17850"
     CDS             1339..2016
                     /locus_tag="VLI90_17850"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HSV16130.1"
                     /translation="MNPITRNHLVTSLLACLAMALLAGCGGSEPQQKDKGFFTSGNRE
                     ADQRADQRMANQKQMAGGSSGNSGATAGNHKSSDSGAIIVPEKRPLFDRLGGADGINH
                     IVEDFVNRALADPRVNWSRAGVKRGGFSIHRGESEQWDASAANVQKLKQHMAQFLSVA
                     TGGPSTYTGKSMHDAHANMHISNPEFDAAMGDMKATLDKLQIANPEQKELLAVIESTR
                     EEIVEKR"
     gene            complement(2023..2925)
                     /locus_tag="VLI90_17855"
     CDS             complement(2023..2925)
                     /locus_tag="VLI90_17855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV16131.1"
                     /translation="MDCVEGGASVSFMLPMTRGKARAFWESVAASAARGERIVIIAED
                     PAGTILGTVQVILGQPENQPHRGDVAKMLVHRRARRRGVGAALLAAAERFALEAGKTL
                     LVLDTVSGSDGDRLYARQGWQRCGEVPNYALWPDGRLCATTFFYKFLGAGTPQSTSDP
                     EFLRQFETCALPFAQWTHRAHVKVAYLYLSAHPFDEALPLMRTGIKRYNAHNHVPESE
                     TSGYNETTTHAFMHLIAASMLAYGATYPTSNADHFYESHPQSHTRHILRLFYSPQQRM
                     HPLAKTRFIEPDLTPLPRFIDGRL"
     gene            complement(3011..3532)
                     /locus_tag="VLI90_17860"
     CDS             complement(3011..3532)
                     /locus_tag="VLI90_17860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV16132.1"
                     /translation="MQIVNCSYAAHAAGILDILNDAIVNSTAIYDYTPRAPETMVGWF
                     DAKSKRDFPVIGAVDDSGELLGFATYGTFRTWPAYKYSVEHSVYVHKDHRGRGIGSTL
                     MRRLIEIAKQQQYHVMLGGIDADNRGSIVLHERLGFVHAGTIRQAGFKFGRWLDLAFY
                     QLLLETPAQPVDG"
     gene            complement(3675..4694)
                     /locus_tag="VLI90_17865"
     CDS             complement(3675..4694)
                     /locus_tag="VLI90_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16133.1"
                     /translation="MRTRFLLAMSLGLCCCAAATASAGEYAVVKTLKIGGDGRWDYIT
                     VDPATKRLFISRSSHTQVVNADDGKLLADLKDTNGVHGVALVPDQNRGFTTNGRDNST
                     TIFDLKTLQPIGTTKTGEGPDGVLYDPFSKCVLTMNGKGHDTSVIDPAAAAGAPVKAT
                     IGLGGKPEGSASDGAGAVFVAVEDASSVAVLDMKEMKLVHTWKIEGGEEPSGLALDAE
                     HHHLFLGCGGNNVMAIMDSQSGKTLGTVPIGKGVDYCAFDPGTGEAFASCGDGTLTVV
                     KETSPGKFEVTQTVKTAQGARTMALDPATHTIYLPTAEFQPATGGGRPQMKAGTFMVV
                     VVAPK"
     gene            complement(4709..>6562)
                     /locus_tag="VLI90_17870"
     CDS             complement(4709..>6562)
                     /locus_tag="VLI90_17870"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV16134.1"
                     /translation="GSATGDAGVAPTAPSTINAKGRVLDATDEFTKPLAGSSLSTIII
                     HVPPAFLVGVFGAFFAALSLSMATSLIISFLVAWLAIPVLAARFLRAKDVKIEEPGRV
                     ARGAYRGYEWIMRRILPWPWLILIVIVPLLWLGYHYYTGVKTGLMPSVDEGGFVIDYI
                     GPPGASITEMDRLLGHVEQILRQTKEVQTYSRRTGFSLGGDIQESNTGDFFVRLKALP
                     RRPIDDVMKDVRQRIVAEIPGLDIDPKQLMEDLIGDLTGQPQPIVVNIFSDDESLLKD
                     LAPKVADALKKISGVNSVETGVVPAGDAYDVEVDRVKASLEGVDPDAITKALGDLLSG
                     AVTTQVQQGPKLIDVRVWTPRSVRKTTDVLQELPIRAPDGHVFPLKRVASFKIDPGQP
                     EITREDLKRVVSVVGRSDRDLGSTIRDVQETLKQPGLIPEGVRYTLGGLYEQQQIAMR
                     GVVRVIIAAGALVFLLLLLLYERFRVAIAIMLISVIATALVFCGLKWTGTELNISSMM
                     GMVMIVGNVTEVAIFYYSEYADFLTTGTVNDRMIAAGNYRMRAIAMTTVAAILALLPL
                     ALDIGHGSAMLQPLAIAIITGLIVQLPLVLIVLPALLVLFRTHRTQPAPQP"
BASE COUNT         1238 a         2087 c         2040 g         1197 t
ORIGIN      
        1 gaccgacaaa tcgtcgactt cacgcactta gccggcggat actcgctaag ctgatccgcc
       61 aagcccatcc agcggtgcgc cgtttgctcc acccacgcct gccgcggtcg gtggattcgc
      121 gaaatgtcgc agacggatga ccagacgaaa acgccggaat cgccccagac gcccaagcgc
      181 aagcgcaggc tgatctggct tatcttgcgc gcgctcggct ggtgcatcat cgggttaatc
      241 ctgatcgccc tgatcgcccg ccccttcatg ccgtgggcgg tgcggtggta cgtcaatcgg
      301 acgttggatc agtcgatcct gtaccgcggg aagatcggcg aactgaacct gcacttgtgg
      361 cgcggcgcgt acaccattca tgatgtccgc atcagcaaga cgaccggcaa cattccggtg
      421 ccgctgtttt cgaccaagga gttggacctg tctgttcagt ggaacgcgct gttacacgga
      481 cgcgtggtcg ggcaggtcaa gatgctgcag ccggaggtga acttcgttga cgatcctgac
      541 cccgccaaca gtgaaaccgg cgcgggtggc ccgtggctga aaatgctgcg cgacctcttt
      601 cctttcgacc tgaaccgggc cgagttgcac gatggcgcca tccacttccg cacataccaa
      661 aaagccacgc cggccgatgt ttatctcagt catgtcgaag cccgcatcga cgatctgcag
      721 aacgtcaacc gctcgctcgc gccattgctg gcgacggtga gcgcgacagc cacggcgatg
      781 gaccagggga agctcgactt caagatgagg ctcgatccct tctcgtacaa cccgacgttc
      841 catctggcgc tgcgattgct cggcctcgat gtcaccaaaa ccaatcccgt tctcgaaacc
      901 tacggcgggt tcaacgtcaa aagcggattc ttcgacctcg tcatcgacat ggattccaac
      961 gaaggccagc tgagcggtta cgttaaaccg ttgttccgcg acatggtgat cttcgatctc
     1021 gtcaaggacg taaagcagga caaggacgcg ctccaggtct tctggcaagc actcgtcggc
     1081 ggcgccgcgg ccatcgtcac caactacaat cgcaaccagc tcggcacgct catcccgttc
     1141 accggcgaac tgaccacgcc gggtgtcgat tacctcgccg ccatcggcaa cgtgctgcgc
     1201 aacgcgttca tccgcgcgta tctgccgcgg ctggaaaaga cccggcagac ggaggacaac
     1261 ctgacgttcg ggccgccgtc gctggccgat ccgatctccg ttgaaaatca gcaatgacca
     1321 ttcacgaagg acccgtttat gaatccaatc actcgcaatc atctcgtcac atcgctgctc
     1381 gcgtgcctgg ccatggcgct gcttgccggg tgtggtggat cggaaccgca gcagaaagac
     1441 aaaggcttct tcaccagcgg caatcgcgaa gccgaccagc gcgccgacca gcgcatggcc
     1501 aaccagaagc agatggccgg gggcagcagc ggtaacagcg gagcgaccgc cggcaatcac
     1561 aagtccagcg acagcggcgc gattatcgtt ccggaaaagc ggccgctgtt cgatcgcctc
     1621 ggtggcgccg acggcatcaa ccacatcgtc gaagacttcg tcaaccgcgc attggccgat
     1681 ccgcgcgtga attggtcccg cgccggtgtg aaacgcgggg gcttcagcat tcatcgcggt
     1741 gaatcagagc agtgggacgc cagcgccgcc aacgtgcaga aactgaagca gcacatggcc
     1801 cagttcctca gcgtcgccac cggcggcccg tcgacctaca ccggaaagtc gatgcacgac
     1861 gcccacgcga acatgcacat cagcaacccc gaattcgacg ccgccatggg cgacatgaag
     1921 gcaaccctgg acaagctgca gatcgccaat ccggagcaga aggaactgct cgccgttatc
     1981 gaaagcacgc gcgaggagat cgtggagaag cgatgaacac gttcacaacc gcccgtcgat
     2041 gaagcgcggc agcggcgtca aatcgggttc gatgaaccgc gtcttggcca gcgggtgcat
     2101 gcgttgctgg ggtgagtaaa acaaccgcag gatgtgccgc gtgtgggact gcggatggct
     2161 ttcgtaaaag tggtcagcgt tcgatgttgg ataggtcgcg ccatacgcca gcatcgacgc
     2221 ggcgatcagg tgcatgaacg cgtgggtggt ggtctcgttg tagccgctcg tttctgattc
     2281 tggcacgtga ttgtgcgcgt tgtaccgctt gatgccggtt ctcatgagcg gcagcgcctc
     2341 gtcgaacggg tgggcactta gatacagata cgcgactttg acgtgagccc ggtgcgtcca
     2401 ctgcgcgaac ggtagcgcgc acgtttcaaa ttgccttagg aattccggat cgctagttga
     2461 ttgcggtgtg ccggcgccga ggaacttata aaagaacgtc gtggcgcaaa gccgaccatc
     2521 cggccacagc gcgtaattcg gaacctcgcc gcagcgctgc cagccttgcc gggcgtaaag
     2581 gcgatcgccg tcgctgccgc tgacggtgtc gagcaccagc agcgtcttgc ccgcctccaa
     2641 tgcaaatcgc tcggcggcgg cgagcagcgc agccccgacg ccgcgccggc gcgcccggcg
     2701 atgcaccagc atcttcgcca cgtcgccgcg gtgcggctgg ttttccggtt ggccaaggat
     2761 gacctgcacc gtcccgagaa tcgtgcccgc cgggtcttcc gcgatgatta cgattcgttc
     2821 tccacgggcc gcgctggcgg cgacgctttc ccagaacgcc cgcgccttgc cgcgcgtcat
     2881 cggcagcatg aagctgaccg atgcgccgcc ttcgacgcag tccatgagca cgtcgcaaag
     2941 cccgtcgatc tcacaatgac caagcgcatc ggcgcgtcga atttgaatcg cacttccgtc
     3001 catcgccgaa tcacccatca accggctgcg ccggtgtttc gagcagtagc tgatagaacg
     3061 ccaggtccag ccaacggccg aatttgaatc cggcctgccg gatggtgccg gcgtggacga
     3121 agccgagccg ctcgtgcagc acgatgctgc cgcgattgtc ggcatcgatc ccgccgagca
     3181 tcacgtggta ttgctgctgt ttcgcgatct cgatcaggcg gcgcatcaat gtcgatccaa
     3241 tcccgcggcc gcggtggtcc ttgtgcacgt agaccgagtg ctcgacggaa tacttgtacg
     3301 cgggccaagt gcggaacgtg ccgtaggtgg cgaagccgag tagttctccg ctgtcgtcaa
     3361 cagcgccgat cacgggaaag tcccgcttcg atttcgcgtc gaaccagccg accatcgtct
     3421 cgggcgcgcg cggtgtgtaa tcgtaaatcg cggtcgagtt gacaatggcg tcgttgagga
     3481 tgtcgaggat gccggccgcg tgtgccgcgt agctgcaatt gacgatctgc acgatgcctc
     3541 ccgtcgcgca aattgacagt gtcatccgcc ggatgctcta agaacgaacg aatccccgca
     3601 tggacatggg ccgccacggc ctcgcggcgg ttttttacgt ccgcaccgcg cggccgcagc
     3661 ggctagcccg acactcactt cggcgccaca accaccacca taaacgtccc cgccttcatc
     3721 tgcgggcgtc cgccgccggt ggcgggttgg aactccgccg tcggcaggta gatcgtgtgc
     3781 gtcgccgggt ccagtgccat cgtgcgggca ccctgggcgg tcttgaccgt ctgggtcacc
     3841 tcgaactttc ccggtgacgt ttccttgacg acggtcagcg tgccgtcgcc gcagctggcg
     3901 aaggcttcgc cggtgccggg gtcgaacgcg cagtagtcca cgcctttgcc gatcggcacg
     3961 gtgccgagcg ttttgccgga ctggctatcc atgatggcca tcacgttgtt cccgccgcaa
     4021 ccgaggaaca agtggtgatg ctcggcgtcg agcgcgagcc ccgacggttc ttcgccgcct
     4081 tcgatcttcc aggtgtgcac gagcttcatc tccttcatgt cgagcaccgc aactgaactg
     4141 gcgtcttcga cggcgacgaa gacggcgccg gcgccatccg acgccgagcc ctccggcttc
     4201 ccgccgagcc cgatcgtcgc cttcaccggc gcgcccgccg cggcggcggg atcgatcacg
     4261 ctcgtgtcgt ggcctttgcc gttcatggtc agcacgcatt tgctgaacgg atcgtacagt
     4321 acgccgtcgg gaccttcgcc ggtcttggtg gtgccgatcg gctggagcgt tttgaggtcg
     4381 aagatcgtcg tcgagttgtc gcgcccgttg gtggtgaacc cgcggttctg atcgggcacc
     4441 agcgcgacgc cgtgcacgcc gttggtgtcc ttcaggtcgg ccagcagttt gccgtcgtcg
     4501 gcgttgacga cctgcgtgtg cgacgagcgc gagatgaaca gccgtttggt cgcgggatcg
     4561 acggtgatgt aatcccagcg gccgtcgccg ccgatcttca gcgtcttcac gaccgcatat
     4621 tcaccggccg acgcggtggc ggcggcacag cagcaaagtc caagcgacat ggccagcaga
     4681 aatcgagttc gcatgacaga tcctccgatc agggttgagg tgcaggttgc gtgcgatgcg
     4741 tgcgaaacag aaccagcagc gccggcagca cgatgagcac cagcggcagc tgcacgatga
     4801 gtccagtgat aatggcgatc gccagcggtt gcagcatggc cgatccgtgg ccgatgtcca
     4861 gcgccagcgg cagcagcgcg agaatggccg cgaccgtcgt catggcgatc gcgcgcatcc
     4921 gataattccc cgcggcaatc atccggtcat tcacggtgcc ggtggtcagg aagtcggcgt
     4981 attcggaata atagaagatc gcgacctcag ttacgttgcc gacgatcatg accatgccca
     5041 tcatcgaaga aatgttcagc tccgtgccgg tccatttcag gccgcagaag acgagcgcgg
     5101 tggcgatgac gctgatgagc atgatggcaa tggcgacgcg gaagcgctcg taaagcagca
     5161 ggagcaggag aaagacgagc gccccggcgg cgatgatgac gcgaacgacg ccgcgcatcg
     5221 cgatctgctg ctgttcgtat aacccgccga gcgtgtagcg tacgccttcg ggaatcaggc
     5281 cgggctgctt caatgtttcc tgcacgtcac ggatggtgga accgagatcg cggtcgctgc
     5341 ggccgaccac cgatacaacc cgcttcaagt cctcgcgggt gatctcgggt tgtccgggat
     5401 cgatcttgaa cgacgcgacc cgcttcagcg ggaacacatg cccatcgggc gcgcggatcg
     5461 gcagttcctg caggacgtcg gtggtcttgc gcaccgacct cggcgtccaa acgcgcacgt
     5521 cgatcagctt gggcccctgc tggacctgcg ttgtcacggc gccgctgagc aagtcgccca
     5581 gcgccttggt gatcgcatcc ggatcgacgc cttcgagcga cgccttcact cggtccactt
     5641 caacgtcgta cgcatcgccc gccgggacga cgccggtttc gacggaattg acgccggaaa
     5701 tcttcttcag cgcatcggcc accttcggcg caagatcttt cagcaggctt tcatcgtcag
     5761 agaaaatgtt gacgacgatc ggctgcggct ggcccgtcag gtcgccgatc aggtcttcca
     5821 tcagttgctt gggatcgata tccagacccg ggatttccgc gacgatgcgc tgtcgcacgt
     5881 ccttcatcac gtcatcgatc ggccggcgcg gcagcgcttt gaggcgcacg aagaaatccc
     5941 cggtgttgct ctcctggatg tcaccgccca ggctgaagcc cgtgcggcgg gaataggtct
     6001 gcacttcctt cgtctggcgc aggatctgct cgacgtggcc gagcagtcgg tccatctcgg
     6061 tgatcgacgc gccgggcggg ccgatgtagt cgatgacgaa gccaccttcg tccacgctcg
     6121 gcatcagtcc ggttttgacg ccggtgtaat agtggtagcc cagccacagc agcgggacga
     6181 tcacgatcag aatcagccaa ggccacggca ggatgcggcg catgatccat tcgtaaccgc
     6241 gatacgcgcc gcgcgctacc cgacccggtt cttcgatctt cacatcctta gcccgcagga
     6301 accgggcggc cagcaccggg atcgccagcc acgcgacgag gaacgagata atgaggctcg
     6361 tcgccatcga cagcgacagt gcggcgaaga acgcgccgaa gacgccaacg aggaacgccg
     6421 gcgggacgtg gatgatgatg gtcgagagcg acgagccggc gagcggcttt gtgaattcat
     6481 cggtcgcgtc gagcacgcgg cccttggcgt tgatggttga gggcgctgta ggggcgacgc
     6541 ctgcgtcgcc cgttgccgat cc
//
LOCUS       DATIMH010001026         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3992424, whole genome shotgun sequence.
ACCESSION   DATIMH010001026 DATIMH010000000
VERSION     DATIMH010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3992424"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VLI90_17875"
     CDS             <1..308
                     /locus_tag="VLI90_17875"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HSV16135.1"
                     /translation="DQAFEKCDAILCPTSPTPAFKAGEKTGDPLQMYLCDVFTVTCNI
                     AGIAGISLPCGFTTGEKPLPIGLQLLGPTFGEERLLRIARMYESATDWHKRRPALAI"
     gene            complement(408..2942)
                     /locus_tag="VLI90_17880"
     CDS             complement(408..2942)
                     /locus_tag="VLI90_17880"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HSV16136.1"
                     /translation="MSRDIAGTDVTSLGALSRRPAVSLVLVFALGIALHDLLPHNAAA
                     MMAITGLVVALAAIEIDAPYRCSALLAIAIALCAAGFAQREHFQYPANHVGQFAGDEP
                     RLAEVELRLIDEPRIITGSFGQLRPLPPKQIVSADVLRVKTWNGWVAASGRTVLQVDQ
                     PHPRLDAGQIVHVYGMLQRPPPPMNPGQFDWSDYYRRQRLLATISARRAENVQIIAEP
                     GPSAVQWLQIKSRRLLAIGFTAKHAIDAAMLRALVLGDRDPQMRDVQEQFMQTGVAYQ
                     MNVSGLHVVLLAIFALWLCACARLRPRYSLAIATVFIALYVTVSLPAFSGVRSLILWF
                     ALAIALFVRRPTNRFQLLALAVLAMLVWNPMDLYSGGFQLSVVAVAGLTLFLPRVREL
                     LLRWRDPHLVAAGSLYRQSIWEAALARPLAMAIKTLEFTFIGWLATLPLIAWQFGQLN
                     PWAMFAGALLLPLVFAALLGGILKILFTLLWPASSSLFALVAGWPIAGVQYAVYLLAK
                     LPGANVPMAAPPVWVIVAYYALLLAPLIPRVPLLTGRRRWCVRCVPFAGVAAMFLLPV
                     FPTLQHPVATVVTPTLRVTLLSVGAGQCAVIEPPGGQPILFDAGSSSISDVARKVIAP
                     FLRSDGRRQIDRIFLSHGDYDHMCAAAELVTAYGVHEVFMSPHFRRNAEGNQPDEVLL
                     DMLDRLDMPPHIIAAGDRVDLGGGAMVDVLWPLTDCNENSNNAGLVLRLSYGGRTILF
                     PADIQDPAFAGVLKHADALPADVLVAPHHGSFERLTPAFVHAVHPTYIVSSNAWRLTN
                     KQKRFDDSVRDYPEYRTNQCGAITITIDQQGKLTLETFLVNGQPKR"
BASE COUNT          643 a          966 c         1014 g          484 t
ORIGIN      
        1 tcgaccaggc atttgaaaag tgcgacgcga tcctctgccc gacatccccc acgcccgcgt
       61 tcaaagccgg cgaaaaaacc ggcgatccgc tgcagatgta cctctgtgac gtgttcacgg
      121 tcacctgcaa catcgccggc atcgccggca tcagcctgcc ctgcgggttc accaccggtg
      181 aaaaacccct gccgatcggc ctgcagctgc tcgggcccac ctttggcgaa gaacggctgc
      241 tgcgcatcgc gcggatgtac gaatcggcga ccgactggca caagcgccgg cctgctttgg
      301 ccatataaag ccgccgatgg tatcggcggt cctaaggaac cccagaccgc ggataacatg
      361 aaccgagacc gcggataata tccgcggctt tatgaggagg gtgtgcgcta gcgtttgggc
      421 tgaccgttga cgaggaatgt ttcgagcgtc agcttcccct gctgatcgat ggtgattgtg
      481 atggcaccgc actggttggt gcgatactcc ggataatcgc ggaccgaatc gtcgaaccgc
      541 ttctgcttgt tcgtcaagcg ccaggcgttg gagctgacga tgtaggtcgg atgcaccgcg
      601 tgtacgaacg cgggggtgag acgttcgaag ctgccgtggt gcggcgcgac cagcacgtcg
      661 gcgggcaggg cgtcggcgtg ctttagcacg ccggcgaacg cgggatcttg aatatcggcg
      721 gggaagagga tcgtgcggcc gccgtagctc aggcgaagga caagtccggc gttgttcgag
      781 ttttcgttgc agtccgtcag cggccaaagc acgtcaacca tcgcaccacc gccgaggtcc
      841 actcgatcgc ccgcggcgat gatgtgcggc ggcatgtcca gccgatccag catgtccagc
      901 agcacttcgt ccggctgatt tccctcggcg ttgcggcgga agtgcgggct catgaaaacc
      961 tcgtgcacgc cgtacgccgt caccagctcg gcggcggcgc acatgtggtc gtagtcgccg
     1021 tggctcagga aaatccggtc gatctgccgc cggccgtcgc tgcgcaggaa cggcgcgatg
     1081 accttgcggg cgacatcgga aatcgatgat gacccggcat cgaacaaaat tggctgtccg
     1141 cccggcggtt caattacggc gcattgaccc gcgccgaccg acagcagcgt gacgcgcagc
     1201 gtcggtgtca ccaccgtcgc caccgggtgc tgcagcgtcg gaaacaccgg caggaggaac
     1261 atcgccgcca cccccgcgaa cggcacgcag cgaacacacc agcgccgccg gccggtcaac
     1321 agtggcacgc gggggatcag cggggcaagc agcagcgcgt agtacgcgac gatcacccac
     1381 accggtgggg ctgccatcgg tacatttgcg ccgggcaact ttgccagcaa atacaccgca
     1441 tattggacac cggcgatagg ccaccccgcg acaagcgcaa acaacgatga ggaagccggc
     1501 catagtaacg tgaatagaat cttcaagatg ccaccaagca gcgcagcgaa taccaacggt
     1561 agcagcagcg cgccagcgaa catcgcccag ggattgagct gaccgaactg ccaggcaatc
     1621 aggggcaagg ttgcgagcca accaatgaac gtgaactcca gcgtcttaat cgccattgcc
     1681 aaaggacgcg ccagtgctgc ttcccagatc gattggcgat agagcgaacc ggcagccacc
     1741 aggtgagggt cgcgccaacg gagaagaagc tcgcgcactc ttggtaaaaa cagcgttagg
     1801 ccggcgacgg cgacgacgct caattgaaag ccgcccgagt aaaggtccat cgggttccag
     1861 accagcattg ccagaaccgc cagcgccaat aattgaaagc gattggtcgg acgtcgcacg
     1921 aacagcgcaa tcgccagggc gaaccacagg atgagcgatc gcacgccgga aaatgccggc
     1981 agcgaaaccg tcacgtagag cgcgatgaac accgtggcga tggcgagcga atagcgcggc
     2041 cgtagccttg cgcaagcgca taaccaaagc gcgaaaatag ccagcaaaac cacatgcagc
     2101 ccactcacgt tcatctgata cgcgacgccg gtctgcatga attgctcctg cacgtcgcgc
     2161 atctgcggat cgcggtcgcc gagcaccaat gcgcgcagca tggcagcatc gatcgcatgt
     2221 ttcgctgtga agccaatcgc cagcagtcgc cgcgatttga tctgcaacca ctgaaccgcc
     2281 gacggccccg gctcggcgat gatctgcaca ttttcggcgc gacgcgcgct gatcgtcgcc
     2341 agcaagcgtt ggcggcggta atagtcggac cagtcgaact gccccgggtt catcggcggc
     2401 ggcgggcgtt gcagcatgcc gtagacgtgc acgatctgcc ccgcatccag ccgggggtgc
     2461 ggttgatcga cttgaaggac ggtgcgacca gaggcggcga cccagccgtt ccacgtcttc
     2521 acgcgcagca cgtcggccga cacgatctgc ttcggcggca gcgggcggag ttggccgaac
     2581 gaaccggtga tgatgcgcgg ctcgtcgatc agccgcagtt cgacttccgc caaccgcggt
     2641 tcgtcgccgg cgaattgtcc gacgtgattg gcgggatact ggaagtgctc gcgctgcgcg
     2701 aagccggcgg cgcagagcgc gatggcgatc gccagcagcg ctgagcacct gtaaggcgcg
     2761 tcgatctcga tcgccgccag cgcgacgacc aagccagtga tcgccatcat cgcggcggcg
     2821 ttgtgtggga gaagatcgtg cagcgcgatg ccgagggcga agaccagcac caacgaaacg
     2881 gcggggcggc gtgagagggc gccgagcgat gtgacgtcag tccccgcgat gtcgcgcgac
     2941 atgagcgggc gtgatgataa cgcgaggttg cggggactgc gatgttgtgc ggagggggct
     3001 gaacgggatg gatcagaatg cacgcgcccg cacggtgccc ctcacgccgg gttcggaggt
     3061 actccgacga cccgggtggt tcgcgccgca ggatccgggt cgtcgcg
//
LOCUS       DATIMH010001027         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_242130, whole genome shotgun sequence.
ACCESSION   DATIMH010001027 DATIMH010000000
VERSION     DATIMH010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242130"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VLI90_17885"
     CDS             <1..701
                     /locus_tag="VLI90_17885"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSV16137.1"
                     /translation="VTVDRPYVEKTLGPRRFEPELAQRTSIPGVATGMAYTPVGGEIL
                     FIESSRVPGRGNITLTGQIGDVMKESATAAYTLVRSRAEQLGIDPKLLADSDIHVHVP
                     AGAVPKDGPSAGTAMFTSLASLMLNKPVRADVAMTGEITLRGLVLPIGGLKEKTLAAL
                     RAGIREVIVPKRNEKDLPDLPQEVRDHLKFHLVDNVDEVLRVALSAAAKSTNGRAKAT
                     MNKTAKRGKIAAAK"
     gene            complement(817..2079)
                     /gene="uxuA"
                     /locus_tag="VLI90_17890"
     CDS             complement(817..2079)
                     /gene="uxuA"
                     /locus_tag="VLI90_17890"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HSV16138.1"
                     /translation="MSHHTTPRGTTPVNLSDPKLHLLEQGFRWFGPNDPVPLSYIRQA
                     GASAVFTSLHQIPYGQFWSRDAIRQRKQAIESAGLRWTAVESVPVHEDIKTGRGDVEA
                     LLQNYAQTLRHLAAEGVNLVIYNFMPVLDWVRTEMRSVLPDESESLHFDPVRFAAFEI
                     HALRRSGAESDYTPEQRQRAAEWWNALSVDDRERFIQTIIDVFPGVRLGLSLDEIRQM
                     LAPYQQMTDAQLRANLGRFLRAIIPVAEEVGVRMAIHPDDPPFSVLGLPRIVSTASDI
                     DSILNMVDSPANGLCFCTGSLSVRSDNDLPAMVRRFAPRIHAAHLRSTQRLPDGSFFE
                     ADHLAGSVDMPAVVRALLDEQDRRRTEKRGNWQIPFRPDHGHTMMDDLGKPVVITPGY
                     SCIGRTRGLAELRGLMLGLRYAELELAG"
     gene            complement(2076..>2776)
                     /locus_tag="VLI90_17895"
     CDS             complement(2076..>2776)
                     /locus_tag="VLI90_17895"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HSV16139.1"
                     /translation="FRKPISYARNIVWNRGERLYTSVVEPTPQGKPRRVGGWGVVPSH
                     ASWTWPGYEGKSLQVQVYSRYDAVRLYLNDALIGEQPTTRDQKFKAVFDVPYTLGTLR
                     TAGVEGGQEVETNIFRTAGAPAALRLTADRASVAADGQDLSFINVEVVDEKGDVQPNA
                     NQLVTFTVTGPARIAGVASGDMSQTDGYQTNARRLFHGRAQVIIRSTRTAGATAVHAS
                     ADGLKESTVEIIAQ"
BASE COUNT          519 a          891 c          884 g          482 t
ORIGIN      
        1 gcgtgaccgt cgatcgtcct tacgttgaga agacgctcgg cccccgccgg ttcgaaccgg
       61 aactggcgca gcgcacaagc atccccggcg tggccaccgg catggcatac acccccgtcg
      121 gcggcgagat cctgttcatc gaatcgagcc gcgtcccggg ccgcgggaac atcacgttga
      181 cgggacagat cggcgacgtg atgaaggaat cggcgacggc cgcctacaca ctcgtgcgat
      241 cgcgcgccga gcaacttggc attgatccaa aactgctggc cgacagcgac atccacgtgc
      301 acgtccccgc cggggcagtg cccaaggacg gccccagcgc cggcaccgcg atgttcacat
      361 cgctggcgtc gctgatgctg aacaagcccg tgcgcgccga cgtcgccatg accggcgaga
      421 tcacgcttcg cggcctggtc ctgccgatcg gcggcctgaa agaaaaaacc ctcgccgcgt
      481 tgcgcgccgg cattcgagaa gtgatcgtgc ccaagcgaaa cgaaaaggac ctgccagacc
      541 tgccgcagga ggtgcgggat catctcaagt ttcatctcgt cgataacgtc gatgaggtgc
      601 tgcgcgtcgc gctgagcgca gcggcgaaat caacaaacgg ccgggcgaag gcgacaatga
      661 acaagacggc gaagcgcggc aagattgcag cggcgaagta atcaggacga cggacgcggt
      721 gttcccaata gatctcgaac acccttttgg cggcggcccg tagggccgag cttccagccg
      781 caataaataa tcttgcattt cggttcctat tgacgtctaa ccagcaagct caagctccgc
      841 ataccgcaat cccagcatca acccccgcag ctccgccaac ccccgcgtcc gcccgatgca
      901 ggagtacccc ggcgtgatga cgaccggctt gcccaagtcg tccatcatcg tgtgaccatg
      961 atcgggacga aacggaatct gccagttgcc gcgcttctcc gtgcgccggc ggtcctgttc
     1021 atcaagcaaa gcgcgcacaa cggccggcat gtcaaccgaa ccggcgagat gatcggcttc
     1081 gaagaacgac ccatccggca ggcgctgcgt gctgcgcagg tgggcggcat gaatgcgcgg
     1141 ggcgaaccgg cggaccatcg cgggcaggtc gttgtcgctt cgcacgctga gcgagccggt
     1201 gcagaagcac aggccattcg ccgggctgtc caccatgttc aagatcgaat cgatgtcgct
     1261 cgcggtcgag acgatgcgcg gcaaaccgag caccgagaac ggcgggtcgt cggggtggat
     1321 cgccatgcgc acgccgactt cttcggcgac cgggatgatc gcccgcagaa accggccgag
     1381 gttcgcgcga agctgcgcat cggtcatctg ctgataaggt gcgagcatct ggcggatctc
     1441 gtccagcgac agtcccagcc gaacgccggg gaagacgtcg atgatggttt gaatgaagcg
     1501 ttcgcgatcg tccacgctca gcgcgttcca ccactcggcg gcgcgctggc gttgctcggg
     1561 cgtgtagtcc gactccgcgc cgctgcgccg cagcgcgtgg atttcaaacg cggcaaagcg
     1621 caccggatcg aagtgcaggc tttcggattc gtccggcaac accgagcgca tctcagtccg
     1681 cacccaatcg agcaccggca tgaaattata aatgacgaga ttcacgccct cggcggcgag
     1741 gtggcggagc gtctgggcgt agttctgcag cagcgcctcg acatcgccgc ggccggtctt
     1801 gatgtcttcg tgcaccggca ccgattcgac cgcggtccag cgcagaccgg cggattcgat
     1861 ggcctgcttg cgctggcgaa tcgcgtcgcg cgaccagaac tggccatatg gaatctggtg
     1921 cagcgacgtg aagacggcgc tggccccggc ttggcggatg tagctgagcg gcaccgggtc
     1981 attcgggccg aaccagcgga aaccttgttc gagcaggtgc agttttggat cgctgagatt
     2041 caccggcgtt gttcctcggg gggttgtgtg gtggctcatt gcgcgatgat ttcaactgtg
     2101 gattctttga gtccatcggc ggatgcatga acggccgtcg cgccggccgt gcgcgtgctg
     2161 cgaatgatga cctgcgcccg cccgtgaaag agccgacggg cgttggtttg atagccgtcg
     2221 gtctgactca tgtcgccgct ggcgacgccg gcgattctcg cggggccagt gacggtgaac
     2281 gtcaccagct ggttggcgtt cggttgcacg tcgcccttct catccaccac ctcgacgttg
     2341 atgaacgata gatcctggcc atccgccgcg accgacgcgc ggtcggcagt caggcgcagc
     2401 gctgccggcg cacccgcagt gcggaagata ttcgtctcca cttcctgccc gccttcaacg
     2461 ccggccgttc gcagcgtccc gagggtgtac ggcacgtcga aaactgcttt gaacttctga
     2521 tcgcgcgtcg tcggttgttc gccgatcagg gcatcgttga gatacagccg aaccgcgtcg
     2581 tagcgcgaat agacctggac ctgcagcgat ttgccttcat agccgggcca agtccagctg
     2641 gcatgactcg gcacaacacc ccagccgccg acgcgccgcg gctttccctg cggcgtgggt
     2701 tcgaccacgc tggtgtacag ccgttcgccg cggttccaca cgatgtttct cgcgtacgaa
     2761 atcggcttgc gaaagc
//
LOCUS       DATIMH010001028         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3419565, whole genome shotgun sequence.
ACCESSION   DATIMH010001028 DATIMH010000000
VERSION     DATIMH010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3419565"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VLI90_17900"
     CDS             <1..1035
                     /locus_tag="VLI90_17900"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HSV16140.1"
                     /translation="LPKGSAGTYWYHPHPHNTTAEQVASGLAGPLLVRSDVDPLAHVP
                     EVCLLVSGIRLEADGRVSPHTALDWAAGRQHDTLLVNGGRMPRHVMRPGATERWRIIN
                     ATAARHFRLALEGHTFTLVGTDGGLLGAPVPGLTEILLAPAQRIEVLVMIAPDPTARY
                     RLRALRYQTDVLGLATYVDDDLMTVVTTTEPPTPPVSIPGQLRPIGDLGIATIRQRVE
                     LSEVTGLCTRSGATVAFLINGRIFDASRTDVYSRVGQVELWDIVNLTGVAHPFHIHGT
                     QFQLVSRQVGQVVTPAPYLAWIDTVLVPPQHSATIKVRQDLPGKRMFHCHILEHEDNC
                     MMGIVDVVAG"
     gene            complement(1295..>1510)
                     /locus_tag="VLI90_17905"
     CDS             complement(1295..>1510)
                     /locus_tag="VLI90_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16141.1"
                     /translation="VALWRSHKAQFTREAFRRNAIASIPSWAFIVWTWYEMLTVGHLP
                     AVWVVLASAALGYGATLVIWWRTRPKT"
BASE COUNT          290 a          475 c          483 g          262 t
ORIGIN      
        1 ctgccgaaag gtagcgcggg gacctactgg tatcaccccc acccgcacaa tacgaccgcc
       61 gagcaggttg cgagtggact agcggggccg ctgctcgtcc gcagcgacgt cgacccgctc
      121 gcccacgtgc ccgaggtctg cttgttggtg agcggcatcc gcctcgaagc cgatggccgc
      181 gtctccccgc atacggcgct cgactgggcc gcggggcggc agcacgatac cttgctggta
      241 aatggcggtc gcatgccgcg ccatgtgatg cggccgggcg caacggagcg ctggcgcatc
      301 atcaatgcca cggcggctcg ccactttcga ctggctttgg agggacacac gttcacgctc
      361 gtcgggaccg acggcggact gcttggcgca cccgtaccgg gcctgacgga gatcctgctt
      421 gcgcccgcac aacgcatcga ggtgctggtg atgattgcgc cggacccaac ggcccgctat
      481 cggctgcgcg cccttcgcta ccagacggat gtgctcggcc tcgccacgta tgtcgacgat
      541 gacttgatga ccgtcgtgac gacgacggaa ccgccgacac cgcccgtttc gatccctggg
      601 cagttgagac cgatcggcga cctcggcatc gcaacgatac ggcagcgtgt agagctcagc
      661 gaagtgacag gtctgtgcac ccgcagcggc gctacggtgg cgtttctcat caacgggagg
      721 attttcgacg cgagccgaac cgacgtttac agccgcgtcg gccaggtgga attatgggac
      781 atcgtgaact tgaccggcgt agcgcacccg tttcacatcc acggcacgca gttccagctc
      841 gtatcgcggc aggtcggcca ggtcgtgaca cctgcaccct atcttgcgtg gatcgatacc
      901 gtgctggtgc cgccgcagca ctccgccacg atcaaggtgc ggcaggacct gccgggcaag
      961 cgaatgttcc attgccatat cctggagcac gaagacaact gcatgatggg gatcgtcgac
     1021 gtggtagcag ggtagcgttt agaagttcgc tgccgtttcg aggcgggcag cgcgcgatgt
     1081 ggcgtgatgc cgtctgccta gcaacggctg caaaaatccg cgctccttct gcaggaggcg
     1141 agcacagtcg ccgcgaatgg agtaccagac gtcattggct gcctccgatt cgttgaacat
     1201 gcggtcacgc atcacgaagg gagtgcagag agaaattgcg gccggccgtt tgtagacgac
     1261 ggtgccaacg acatcggttc gacataacgc agcgtcatgt cttcggtcgc gtccgccacc
     1321 agatgaccaa cgtcgcaccg tagccgagcg ccgcgcttgc caggacgacc caaactgccg
     1381 gcagatggcc aacggtcagc atctcgtacc aagtccaaac gatgaaagcc catgaaggga
     1441 tcgacgcgat cgcattgcgg cgaaaggctt cgcgagtaaa ttgcgctttg tgggaacgcc
     1501 agagggcgac
//
LOCUS       DATIMH010001029         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1111806, whole genome shotgun sequence.
ACCESSION   DATIMH010001029 DATIMH010000000
VERSION     DATIMH010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111806"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VLI90_17910"
     CDS             <1..218
                     /locus_tag="VLI90_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16142.1"
                     /translation="DDSRFYDVPVAIYSALTDDTSIERAMKLGACDYIIKGVGWPETY
                     ERIRAHLPSSIDEAPTETPESDIATAV"
     gene            257..931
                     /locus_tag="VLI90_17915"
     CDS             257..931
                     /locus_tag="VLI90_17915"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HSV16143.1"
                     /translation="MLSLNPDESRVLGVLIEKATTTPEQYPLSLNAVTNGSNQKNNRD
                     PVRAMTEDECFEALESLRAKGLVVRVDTPGSRVHKYRHNAGDLLHARTAELAILAELL
                     MRGPQTVGELRGRASRMAPLESIEVVQGLLGAMMTREEALVKEIPPSPGSRALRYVQL
                     LCPDLHPLDAPAAATATPTSAPAVTRGGDPTLSDRVARLEAEVAELRGSLQQLMEQLG
                     VPRSAL"
     gene            1099..2100
                     /gene="hemB"
                     /locus_tag="VLI90_17920"
     CDS             1099..2100
                     /gene="hemB"
                     /locus_tag="VLI90_17920"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HSV16144.1"
                     /translation="MNESPLDLPLRPRRSRIQPQLRRMLQRVVLRRSDIIVPIFVTEG
                     SGVRREVSSMPGVFQMSIDNAVPWLSKRAEEGFLTYLIFGVVDRNKKDELGSEALNDG
                     NVVCRLLRAAHEKKLPMAGITDLCFCEYTNHGHCGPVTDDKTTVKNDETVRRLVKQAV
                     NHAKAGAAVIAPSGMMDGTVGALRAGLDAAGYRDVAVLSYAVKYASAFYGPFRDAADS
                     APAYGDRRSYQMDPARGLIEALREVDLDVKQGADMVMVKPAGAYLDIIAAVRRAVNLP
                     VVAYQVSGEYAMIEAASRNGWLDGDRAVLESLIAIKRAGADLIITYYAEKLAKLLPA"
BASE COUNT          410 a          675 c          698 g          355 t
ORIGIN      
        1 aggacgactc gcgcttttac gacgtcccgg ttgccatcta ttctgccctg accgatgaca
       61 cttccatcga gcgcgcgatg aagctgggcg cgtgcgacta catcatcaaa ggcgtgggtt
      121 ggccggagac gtacgagcgc atccgcgcgc atctgccgag ttcgatcgac gaagcgccga
      181 cggaaacgcc ggagtccgac atcgccacgg ccgtgtgatt gaccagaccg cgcggcgtgt
      241 cgtacgctcc gccggcatgt tgtcattgaa ccccgatgaa tcccgcgtgt taggcgtgct
      301 gatcgaaaag gcgacgacca cgccggagca gtatccgctg tcgctgaacg ccgtcaccaa
      361 cggatcgaat cagaagaaca accgagatcc cgttcgcgcg atgaccgaag acgagtgctt
      421 cgaggcgctg gaatcgttgc gcgctaaggg tctggtggtt cgcgtcgata cgcccggcag
      481 tcgcgtgcac aaataccgcc acaacgccgg cgacttgctc catgcgcgta ccgcggagct
      541 ggccatattg gctgaactgc tgatgcgcgg cccgcaaacg gtgggggaac tgcgcggccg
      601 cgccagccga atggcgccgc tggagtcgat cgaagtcgtg cagggattgc tcggggcgat
      661 gatgacgcgc gaagaggcgc tggtgaagga gatcccgcca tcgcccggct cgcgggcgct
      721 gcggtatgtg cagttgctct gcccggatct gcatccgctg gacgcacccg cggcggcgac
      781 ggcgacgcca acgtcggctc cagctgtcac acgtggaggc gatccgacgc tatcggatcg
      841 agtggcacgc cttgaggccg aggtggcgga gctgcgtgga tcactgcagc aactgatgga
      901 gcaactgggc gtaccgcgat ccgcgctgtg acctctcccg agtcaaaact taatagccgc
      961 aggcgacagc cagcgggggg ctttggaaaa ccagcccggc tggtactcgc aagcccccgc
     1021 tggctgtcgc ctgcggctat caaagcggct cgcatggcgc ggcattcgcg cccattcgca
     1081 ctataattca attcaaccat gaacgaatca cccctcgatc tcccgctgcg cccgcggcgg
     1141 tctcgtatcc agccgcaact tcgccggatg cttcagcgcg tcgtcctccg ccgcagcgac
     1201 atcatcgtgc cgatcttcgt caccgaaggc agcggtgtcc ggcgggaagt gtcgagcatg
     1261 cctggcgtgt tccagatgtc gatcgacaac gccgtgccct ggctgagcaa gcgcgcggaa
     1321 gaaggcttcc tcacctacct catcttcggc gtcgtcgatc gcaataaaaa agatgaactg
     1381 ggcagcgaag cgctcaacga cggcaacgtc gtctgccggc tgctgcgcgc ggcgcacgag
     1441 aaaaagctcc cgatggccgg catcaccgac ctttgtttct gcgagtacac caaccacggc
     1501 cactgcgggc cggtgacgga cgacaagacc acggtgaaga acgacgagac ggtccgccgg
     1561 ctggtgaagc aggccgtgaa ccacgcgaag gctggcgccg cggtgatcgc cccgagcggc
     1621 atgatggacg gcacggtggg cgccctgcgc gccggcctcg acgccgccgg atatcgcgac
     1681 gtggcggtcc tgtcatacgc cgtgaaatac gccagcgcct tctacgggcc atttcgagac
     1741 gccgccgatt ccgcgccggc atatggcgat cgccgctcat atcagatgga cccggcgcgc
     1801 ggactcatcg aagcattgcg cgaagtcgat ctcgacgtga agcagggggc cgacatggtg
     1861 atggtgaagc ccgccggggc gtacctggac atcatcgccg cggtgcggcg ggcggtgaac
     1921 ctgccggtcg tcgcatatca ggtcagcggc gaatacgcga tgatcgaagc cgcctcgcgc
     1981 aacggctggc tcgatggcga tcgagcagtg ctggaatcgc tgatcgccat caaacgggcg
     2041 ggggcggatt tgatcatcac gtattacgcg gagaagttgg cgaagcttct gccggcatga
     2101 attcatcgct atttgatagc cgcaggcgac agccagcg
//
LOCUS       DATIMH010001030         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1402863, whole genome shotgun sequence.
ACCESSION   DATIMH010001030 DATIMH010000000
VERSION     DATIMH010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402863"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VLI90_17925"
     CDS             <1..912
                     /locus_tag="VLI90_17925"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSV16145.1"
                     /translation="RGIGDEALIASVQPQLRLQRERGSDLTIFSPRAAGMAHHVGLEA
                     TSQQWARASNDIIHRLCTLLPDNFVGVGQLPQSPGVKPENCIPELERMVNELGFVGVN
                     LNPDPSGGYWKDPPMTDRYWYPIYEKLVELDIPAMIHVTGSCNPNFHATGAHYINADT
                     TIFMQLIQGNLFKDFPTLKFVIPHGGGAVPYHWGRYRGLSMMMKKPPLVEYLMKNVYF
                     DTCVYHQPGIDLLAKLVPIDNILFASEMIGAVQGIDPETGHYFDDTRRYVDAAAGLSD
                     ADKHKIFEGNARKVYPRLDKILKSRGL"
     gene            970..1824
                     /locus_tag="VLI90_17930"
     CDS             970..1824
                     /locus_tag="VLI90_17930"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HSV16146.1"
                     /translation="MALPRLNGIIRQLEAGKHSVTTFQPIDITTAVEITQSKYDGVVF
                     EGEHQGWDITNLRHALQYMLNRAHIAKGGVAPAVTPTARIPANGCEMNQFMAKQALDI
                     GCYGIVFPHVSTVQEAYNAVAACRYPRLKEKPLYEPAGIRGDGPMQACRYWGVSQQEY
                     YQVADVWPLAPQGEILVVIQIEDTMGIENLDDMLKNVPGIGVVLIGEGDLSQELGYPR
                     QTEHKVVLDAMAKVVDTCKKHNVIVGHPHVSKANAERVISEGYRYLMAGAPRNYGELN
                     DTLKAAGR"
BASE COUNT          384 a          608 c          541 g          299 t
ORIGIN      
        1 cgcggcatcg gcgacgaggc cctgatcgcc tcggtccagc cgcagctgcg cctgcagcgc
       61 gagcgcggca gcgacctgac gatcttctcg ccacgcgcgg ccggcatggc gcaccacgtc
      121 ggccttgaag ccaccagcca gcaatgggcg cgcgccagca acgacatcat tcaccgcctg
      181 tgcacgctcc tgcccgacaa tttcgtcggc gtcggccagc tgccgcaatc gcccggcgtg
      241 aagccggaga attgcattcc cgaactcgag cgcatggtca acgaactcgg cttcgtcggc
      301 gtgaatctca accccgatcc gtcgggcggc tactggaagg atccgcctat gacggaccgc
      361 tactggtatc cgatctacga gaagctcgtc gaactcgaca ttccggcgat gatccatgtg
      421 acgggttcgt gcaacccgaa tttccacgca accggcgcgc attacatcaa cgccgacacg
      481 acgatcttca tgcagctcat ccagggcaat ctgttcaagg atttcccgac gctgaaattc
      541 gtcatcccgc atggcggcgg cgcggtgccg tatcactggg gccgctatcg tggcctgtcg
      601 atgatgatga agaagccgcc gctcgtcgaa tacctgatga agaacgtcta ttttgacacc
      661 tgcgtctatc atcagccggg catcgacctg ctcgccaagc tcgtgccgat cgacaacatc
      721 ctgttcgcgt cggaaatgat cggagccgtg cagggcatcg atcccgaaac cggacattac
      781 ttcgacgaca cgcgccgtta cgtggacgcg gccgcgggcc tgtcggacgc cgacaagcac
      841 aagatcttcg aaggcaatgc gcgcaaggtc tatccgcgcc tggacaagat cctcaagagc
      901 cgcgggctct gatcatcttc gggcggcggg cgaccgccgc ccttctcaag aaaacacccg
      961 gagacaaaaa tggccctgcc ccgcctcaac ggcatcatcc gccagctcga agccggcaag
     1021 cattcggtga cgaccttcca gccgatcgac atcacgaccg cggtggaaat cacgcagtca
     1081 aaatatgatg gcgtcgtgtt cgaaggcgaa catcagggct gggacatcac caacctgcgc
     1141 cacgcgctgc aatacatgct gaaccgcgcg cacatcgcca agggcggcgt cgcgcccgcc
     1201 gttacgccga cggcgcgcat tccggcgaat ggctgcgaga tgaaccagtt catggccaag
     1261 caggcgctcg atatcggctg ctacggaatc gtcttcccgc atgtctcgac cgtgcaggaa
     1321 gcctataacg cggtcgcggc ctgccgctat ccgcgcctca aggaaaagcc gctctacgag
     1381 cccgcgggca ttcgcggcga cgggcccatg caggcttgcc gctactgggg cgtcagccag
     1441 caggaatatt accaggtcgc cgacgtctgg ccgctcgccc cgcagggcga aatcctcgtc
     1501 gtcatccaga tcgaggacac gatgggcatc gagaacctcg acgacatgct gaagaacgtg
     1561 ccgggcatcg gcgtcgtgct gatcggcgaa ggcgatctca gccaggaact cggctacccg
     1621 cgccagaccg agcacaaggt cgtgctcgac gcgatggcga aggtggtcga cacctgcaag
     1681 aagcacaacg tcatcgtcgg ccacccgcat gtgtcgaagg ccaacgccga acgcgtgatt
     1741 tcggagggct accgctatct gatggcgggc gcgccgcgca attacggcga actcaacgat
     1801 acgctgaaag cggcaggacg ctgatcgaat ac
//
LOCUS       DATIMH010001031         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_534508, whole genome shotgun sequence.
ACCESSION   DATIMH010001031 DATIMH010000000
VERSION     DATIMH010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534508"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2906)
                     /locus_tag="VLI90_17935"
     CDS             complement(<1..2906)
                     /locus_tag="VLI90_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16147.1"
                     /translation="MQRVRRHLLLPTVITGIIGAAAGSASRALAAGMSDVLGASNINP
                     GYYFTTQDAVNEATNQLVGMGSTTVKLEMGIQDINTTWSDGSIIYPGQTSPPPPSKYA
                     WNTPTWPAKSTLSLVNLAQTTYFSKAFSNPTIKNYVITTYSPNVPSSGGDGTEFWLNG
                     MTASEKAAETASFYNYTKYLLQTYNGTGKTFYLENWEGDWALKDPGRNTQPSPTAVQG
                     MIDWFNARQAGINEAKHDFGAASNVKVYQTIEVNRVADAINGDVFNGQTGWATVTNDV
                     LPYTNVDFASYSSYDTQQVTSGATSYASAVQYLANHLPATAVNGQNTHSVYVGEFGLA
                     EDSAGITTVNNMMNNVLNTVKADGMPMALYWEVYSNEIKSETGLPAGNSGDNSNTKGF
                     YFVKPDGTPATAWNQYRYLLSNSDPTQSSTAAIVRNGGLHLAYASDFTKTGATLGAAW
                     TTNNSGGAMSVGITNGQVQMSTVNGSATPTGQATLNLVSALGRGLNPGEYVQFTLNRQ
                     QDAGIVGFSAFGLNHGSGIGAGNQPLNVFSSGINSTGGTWHYISFNPTGTTAAPSTTY
                     SWNTADTLGLKLLSADGNFATIAYYVNGSYSGSWLYPTTAKTLDSFSLFAQSNTTGNG
                     FGFNNLSIYTTGTAAASTAYQWDPTHNLAGGSGTWDGSTTANFSNGSADTTWNNATLA
                     DNVTFGGTAGTVTVSGNVTASTLKFATPNYTLSGGTITMMGNQTINATSGNATINSVI
                     AGNTGVTITGAGSVTLGGNNTFTGGVFVTGGGTAAFATDANLGGAGQPITLDGGTLSY
                     TGTTAPTLTRAITINAGGGTINNVGNGATGKFTLSGTNLINGSGNLTKAGPGWLTLFG
                     SNTGTFTGNWTISGGVLEVGTASLVGTGSIAVNAGAELVPSNGTVLTNAITVAGGTIG
                     ADNATGGFAGPITVTAASNVRLGNFWNATSQSTNITGNLSGSGKLTTVNASGAVT"
BASE COUNT          534 a         1009 c         1089 g          735 t
ORIGIN      
        1 gtcaccgcgc ccgatgcatt gaccgtcgtc agcttgccgc tgccgctcag attgccggtg
       61 atgttggtgc tttggctcgt cgcgttccag aagttcccca gccgaacgtt cgacgccgcg
      121 gtgaccgtga ttggccccgc gaatccgccg gttgcgttgt ccgcgccgat cgttccgccg
      181 gcgacggtga tcgcgttggt cagcaccgtg ccgttcgatg gaaccagttc cgccccggca
      241 ttgacggcga tggaaccggt gccgacgagc gaagccgtgc cgacttccaa tacgccgccg
      301 ctgatcgtcc agttgccggt gaacgtgccg gtgttgctgc cgaacagcgt caaccaaccg
      361 gggcccgcct tggtgagatt gcccgagccg ttgatcaggt tcgtcccgct gagggtgaac
      421 ttgccggttg cgccgttgcc gacgttgttg atcgtcccgc cgccagcgtt gatggtgatc
      481 gcgcgcgtca acgtcggcgc agtcgtgccc gtataactca gcgtcccgcc atcgagcgtg
      541 atcggctggc ccgcgccacc gaggttcgca tccgtcgcaa acgccgcggt gccgccgccg
      601 gtgacgaata ctccgcccgt gaaggtattg tttccgccca gcgtcacgct gccggcgccg
      661 gtgatcgtta cgcccgtgtt gccggcaatt acgctgttga tcgtcgcgtt cccgctggtc
      721 gcgttgatgg tttgattgcc catcatcgtg atcgtcccgc cgctcagcgt gtagttcggc
      781 gtggcgaact tcagggtact ggcggtgacg ttgccggaga ccgtgacggt gccggcggtg
      841 ccgccgaagg tcacgttgtc cgccagcgtc gcgttgttcc acgtcgtgtc ggcgctgccg
      901 ttggaaaagt tcgcggtggt cgaaccgtcc cacgtcccgc tgcccccggc caggttgtga
      961 gtcggatccc actgatacgc cgtgctcgcc gcggcggtgc cggtcgtgta gatcgacagg
     1021 ttgttgaagc caaacccgtt gccggtggtg ttcgattgcg cgaagaggct gaagctgtcc
     1081 agcgtctttg ccgttgtggg atagagccac gatccgctgt agctgccgtt gacgtaatac
     1141 gcgatcgtcg caaaattccc gtcggccgac aggagtttca ggcccagcgt gtcggccgtg
     1201 ttccagctgt acgtcgtgct cggcgccgcc gtcgtgccgg tgggattgaa cgagatgtaa
     1261 tgccaggtcc cgcccgtgct gttgatgccg gacgagaaga cgttcaacgg ttgattgccc
     1321 gcgccgatgc ccgagccgtg gttcaacccg aacgcgctga agccgacgat gcccgcgtcc
     1381 tgctggcggt tcagcgtgaa ctgcacgtac tcgcccggat tcagcccgcg acccagcgcg
     1441 ctgaccaggt tcaacgtcgc ctgcccggtc ggcgtggccg agccgttgac ggttgacatc
     1501 tgcacctgac cattggtgat gccgacgctc atcgcgccgc cggagttgtt ggtcgtccac
     1561 gcggcgccca gcgtcgcgcc ggtcttggtg aagtcgctgg cgtaggccag gtgcaacccg
     1621 ccgttgcgca cgatggcggc ggttgacgac tgcgtcggat cgctgttgga cagcagatag
     1681 cgatactgat tccacgccgt cgccggcgtg ccgtcgggct ttacgaagta gaagcccttg
     1741 gtgttggaat tgtcgccgct gttccccgcg ggcaggccgg tctcgctctt gatctcgttg
     1801 gaatagacct cccaatacaa cgccatcggc atcccgtcgg ctttgacggt gttcaggacg
     1861 ttgttcatca tgttgttgac ggtggtgatg ccggcgctgt cctccgccaa gccgaattca
     1921 ccgacgtaca cgctgtgcgt gttctgcccg ttcaccgccg tcgccggcag gtgattggcc
     1981 agatactgca cggcgctggc gtagctggtg gcgccgctgg tgacctgctg cgtgtcgtaa
     2041 gacgaatacg acgcgaaatc gacgttggtg tacggcagca cgtcgttggt gacggtggcc
     2101 caaccagtct ggccgttgaa gacgtcgccg ttgatggcgt cggcgacgcg gttcacttcg
     2161 attgtttgat aaacttttac gttactcgct gcgccaaagt cgtgcttggc ttcgttgatg
     2221 ccggcctggc gggcgttgaa ccagtcgatc atgccttgca ccgccgtcgg cgacggttgc
     2281 gtattgcggc ccggatcttt cagtgcccaa tcgccttccc agttctccag gtaaaacgtc
     2341 ttgccggtgc cgttgtaggt ctgcagcagg tatttggtgt agttgtagaa cgacgccgtc
     2401 tccgccgcct tttcgctggc ggtcatgccg ttcagccaga actccgtgcc gtcgccaccg
     2461 ctgctgggca cgttcggcga atacgtggtg atgacgtagt tcttgatggt cggattcgaa
     2521 aatgccttgc tgaagtacgt cgtctgcgcc aggttcacca gcgagagcgt gctcttggcc
     2581 ggccaagttg gggtgttcca cgcgtacttc gacggcggcg ggggcgaggt ctggccggga
     2641 tagatgatcg agccgtcgga ccaggtcgtg ttgatgtcct ggatgcccat ctccagtttc
     2701 acggtggtgg agcccatgcc caccaactgg ttggtcgcct cgttcaccgc atcctgggtg
     2761 gtgaagtagt aacccggatt gatgttggaa gcgcccagca catcagacat gccggcggcc
     2821 agggcgcgcg aagcgctgcc ggcggcagcg ccgataatgc cagtgatgac cgtgggtaac
     2881 agcaggtgcc gacgaacccg ttgcatggac atcctcttta ccgcggggct tagggggtgg
     2941 gacagcgacc cgcgtgcgtg tacgcccgaa agcgaagcgt gtggtataaa taaacaggct
     3001 actcggccgc ctccgggccc cgcgtgctat tagaatatac cacggctggg gcgaccacaa
     3061 tgaaataatt cattgaagtg aaaaatgttt catgtgcatc gaatgcacgg taggggccgg
     3121 cacgtggatg tgctcggtcc tcgtcgaaag agtcccacgg cagagccgtg ggctttgtct
     3181 ggtgcttaac aaaaaaccca cggctttgcc gtgcggcgtg ttctcgattt ggagcttcta
     3241 aaaagactgg tgtggcttgt agctgcgacg cctgcgtcgc cttgtttttc taaagcgaag
     3301 aggcgacgca ggcgtcgcag ctacaagcat ttctagaagc cctttagact aacgactcgt
     3361 tcatggc
//
LOCUS       DATIMH010001032         1664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1982961, whole genome shotgun sequence.
ACCESSION   DATIMH010001032 DATIMH010000000
VERSION     DATIMH010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982961"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1664)
                     /locus_tag="VLI90_17940"
     CDS             complement(<1..>1664)
                     /locus_tag="VLI90_17940"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HSV16148.1"
                     /translation="VFDFGSGTTSYMFLTPDAGGTNAIRFAITNGGNGSEQRLDGPAM
                     TAGTWTHIAVTLAGNTGTLYVNGTAVATNTGMTIHPASFGGTISHDYLADSQFANDPS
                     LLGSIDDFRLYNRALPAAQVAELADPTVINDATAAASPVTGTSTTLSALGFDVTSGES
                     ALTYTWATTGTPPAQVTFSANGTNAAKNTTATFAAAGTYNFTVTITNPGGGTTSSSVS
                     VVVNQTLSNVTVTPGSPTISNNATQSFNATGRDQFGALMAAAPTFAWTIDSGGVGSVD
                     PSTGLYTAPASGPGSATVRATSGGKSGTASIIVILSNIAGTAGDDAIRLVRSGSSLAV
                     YVNNANAPAYTVPFSSLGALTISAGTGNDSITLDFSGGASPVPSAGLSVDGAGGAGDS
                     LIITGTSADDSASVSASAVVFNNSTITYSNIEAIVINGGGGADVLTQSAAPSAVLAFN
                     GGGGGDVLNINAGAFSFAGDAAVNSPALAVNVANAGSSASFSGTQHLSALNISAGATG
                     TMAAVAAWSSPSVMNVTALTISGVGSAFDLHNNELLSSVTLASIRG"
BASE COUNT          255 a          546 c          558 g          305 t
ORIGIN      
        1 cccgcgaatg ctcgcgagcg tgactgaact cagcagctcg ttgttgtgca gatcgaacgc
       61 ggagccgacc ccgctgatgg tgagcgccgt cacgttcatc accgaaggtg aagaccacgc
      121 cgccacggcg gccattgtcc ctgtcgcgcc ggccgagatg ttcagcgctg acagatgctg
      181 agtcccgcta aagctggccg acgacccggc gttggcgacg ttcaccgcga gagcaggcga
      241 attgaccgcc gcatcgccgg cgaagctgaa cgcgccggcg ttgatgttca gcacgtctcc
      301 cccaccgccg ccgttgaacg ccagtactgc gctgggtgca gccgactgcg tgagcacgtc
      361 ggctccgccg ccgccgttga tcacgatcgc ctcgatgttc gagtaggtga tcgtcgaatt
      421 gttgaaaaca acggccgatg ccgagacgct cgcgctgtcg tcagcgctgg tgccggtgat
      481 gatcaacgaa tcgccggccc cgcccgcgcc gtccacgctt aagcccgcgc tcgggaccgg
      541 gctggccccg ccggaaaaat cgagcgtgat cgagtcgttg ccggttcctg cactgatcgt
      601 cagcgcgccc agcgatgaga acggaaccgt atacgcgggg gcgttggcgt tgttgacgta
      661 gaccgccaac gacgagccgc tgcggacgag gcggatcgca tcatcccccg cggtgccggc
      721 aatgttgctc aggatgacga tgatgctggc cgtgccgctc ttgccgccgc tggtggcccg
      781 aacggtcgcc gatccgggac cggatgccgg cgcggtgtac aggcccgttg acggatcaac
      841 gctgcccacc ccgccgctgt cgatcgtcca cgcgaatgtt ggggccgcgg ccatcagcgc
      901 gccaaactga tctcgccccg tcgcgttaaa tgactgcgtc gcgttgttcg atatcgtcgg
      961 tgatcccggc gtcaccgtca cattgctaag cgtctggttc acaacgacgc tgacgctgct
     1021 gctcgtcgtc ccgccgccgg ggttggtgat cgtcaccgtg aagttgtacg tccccgcggc
     1081 ggcgaacgtg gccgtcgtgt tcttcgcagc attcgtgccg ttcgcgctga acgtcacctg
     1141 cgccggtggc gtgcccgtcg tcgcccaggt gtacgtcagc gcggattcgc cgctcgtgac
     1201 atcgaagccg agcgcactga gcgtcgtcga agtgcccgtg accgggctgg ccgccgcggt
     1261 ggcgtcgttg atgacggtgg ggtccgccag ctccgcgacc tgcgccgccg gcagcgctcg
     1321 gttgtacaag cggaagtcgt cgatgcttcc cagcaggctc gggtcgttcg caaactgcga
     1381 gtccgccagg taatcgtggc tgatcgttcc accaaagctg gcgggatgaa tcgtcatgcc
     1441 ggtgttggtc gcgacggccg tgccgttgac gtacagcgtg ccggtgttgc ccgccagcgt
     1501 gacggcgatg tgcgtccagg ttcctgcggt catcgccgga ccgtcgaggc gctgctcgct
     1561 gccgttgccg ccgttggtga tcgcgaagcg aatcgcgttc gttcccccgg cgtcgggcgt
     1621 gaggaacatg tagctggtgg tgccggaacc aaagtcgaag acgc
//
LOCUS       DATIMH010001033         3528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1983044, whole genome shotgun sequence.
ACCESSION   DATIMH010001033 DATIMH010000000
VERSION     DATIMH010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983044"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..402)
                     /locus_tag="VLI90_17945"
     CDS             complement(103..402)
                     /locus_tag="VLI90_17945"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HSV16149.1"
                     /translation="MSSSHSPSTPAHRRQCYFSGRVQGVGFRYTVKNIAIQHNVSGYV
                     RNLPDGRVELVLEGPDQEIDQVVDAVSNRMTGFIKKVDANTAPATGEFDYFQIRH"
     gene            complement(703..1701)
                     /locus_tag="VLI90_17950"
     CDS             complement(703..1701)
                     /locus_tag="VLI90_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16150.1"
                     /translation="MQRSQTKSGVRHLIWFGVLLAGCAASTPKMVLEGPTAPAAYHAD
                     PWSFKQDKGALLKSPHYDIYTTIDTPDVQRSVVQVMEGALGEYEKIAPGVPVSDKPMD
                     CFVFANRQEWMEFTRQHTGPDSNVYLQINRGGYTIRDWYVAYYVGDAATYSVAAHEGW
                     HQFVSRNFKGRLPPFLEEGIATMFEDVDWRTDLPRWNLARNRSRVQSLRRAIESNSIY
                     PLGELVGMHAGNVVNQSGNRIEAFYAESWAFATFLYAGDNAKYRPALRRMMSDTADGT
                     VFDPTGVHQNWRLPWTPAGVKPMLEHYLGMNLDAIDAEYQKYIRKIAFDDYQAQWN"
     gene            1716..2399
                     /locus_tag="VLI90_17955"
     CDS             1716..2399
                     /locus_tag="VLI90_17955"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSV16151.1"
                     /translation="MPSTRLDIISIGTLSRNRLWNETAVVRTAHATTTLIRAGNRNVL
                     VDPGLPAAALGARLYERTGMKPEQIETVFLTNSRPAHRAGLSLFTKAKVLIHEIEQQS
                     ARQQLQQLIDQAPDEDIDRAHLKGELDLIESFKPADDEIADGVDLFPLFGYTPGTCGL
                     LVSATLTTTLIAGDAVPTLDHFLAGQVLPDSWDIKAAHESMAEVYEIADLIVPGHDNL
                     FMNPRSQGV"
     gene            2445..2645
                     /locus_tag="VLI90_17960"
     CDS             2445..2645
                     /locus_tag="VLI90_17960"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HSV16152.1"
                     /translation="MASNLNIDERLLSQAVKTGGHKTKREAVNSALEEYVRHRERQKI
                     TELFGTVEYEPGHDPKNGRRKR"
     gene            complement(3052..>3528)
                     /locus_tag="VLI90_17965"
     CDS             complement(3052..>3528)
                     /locus_tag="VLI90_17965"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSV16153.1"
                     /translation="FGLEVHPTEIAFDIVTAQRAIDAVKGHKRFGFNFDPSHLIYQHV
                     DYVKFIRTFANRIYHVHMKDAWFGHGDGTVGVFGGHTTFADPRRYWDFRSLGHGDVNF
                     EEIIVALNDIGYAGPLSVEWEDSRMDREHGAKEAAAFVKRVDFAPSKIAFDAQFDR"
BASE COUNT          709 a         1089 c         1039 g          691 t
ORIGIN      
        1 taaatggcaa atcgaaattg gtaaatgggg ccggacaacg acagatatat gcgcgcagaa
       61 cggaacagtt gcgactgctg gaggcttaaa ttgtgaatgg gatcagtgcc ggatctggaa
      121 gtaatcgaat tcgccggtgg cgggagcggt gttggcatcg accttcttga tgaagcccgt
      181 catgcgattg ctgacggcgt ccaccacctg atcgatctcc tgatcaggcc cttccagcac
      241 cagctcgacc cgcccatctg gaagattcct cacataaccg ctgacattgt gctggatggc
      301 gatgttcttg acggtatagc ggaaacccac cccttgaacg cgaccgctga agtaacactg
      361 ccggcggtgg gccggggtgg acggcgaatg actcgaactc atgagatgag gcatcattga
      421 tcgctccgtc cgccgatcgg caagcatgag cgcgatatta tcacgctgct gccggatcgc
      481 caagataatt ttggggcgcg ccttaatgct cggtccgctt gatgcggagg gcgacgggca
      541 ggcggagcga atgcggctcc ttccccctct cccggtacgc cgctgatcgc tagggttcca
      601 aaatcaccct cacccttacc ctctcccggt gtaccgacgg tgtaccggga gaggcgacaa
      661 gagcgcgtac tgcgcgacat atgtttgtct ggggactgac ggtcaattcc actgggcctg
      721 atagtcgtca aacgcgatct tgcggatata tttctgatac tcggcgtcga tcgcatcgag
      781 gttcatcccc agataatgct cgagcatcgg cttcaccccc gcgggggtcc atggcagccg
      841 ccagttttgg tggacgccgg tggggtcgaa gacggtgccg tcggcggtat cggacatcat
      901 ccgtcgcagc gccggacggt actttgcgtt gtcgccggcg tacaggaatg tcgcgaaggc
      961 ccagctctcg gcgtagaacg cctcaatgcg attgccggat tgattgacga cgttgccggc
     1021 gtgcatgccg acgagttcgc ccagcggata aatggaattg ctttcgatgg cccgccgcag
     1081 tgactgcacg cgcgagcgat tgcgcgccag gttccaccgc ggcaggtccg tgcgccaatc
     1141 cacgtcctcg aacatcgtcg cgatgccctc ttccagaaac ggcggcagac gccccttgaa
     1201 gttccgcgat acgaactggt gccaaccttc atgggccgcg accgaatacg tggcggcatc
     1261 gccaacgtaa taggcgacat accagtcgcg gatcgtgtac ccgccgcggt tgatttgcag
     1321 atacacgttc gaatccggac cggtgtgctg gcgggtgaac tccatccact cctggcggtt
     1381 ggcgaagacg aagcagtcca tcggcttatc gctcaccggc acgccggggg caatcttctc
     1441 gtattcaccc agcgccccct ccatcacctg caccacggat cgctgcacat ccggcgtgtc
     1501 gatcgtcgtg taaatgtcgt aatgcggact cttcaacagc gcgcccttgt cctgcttgaa
     1561 cgaccaggga tcggcgtgat acgccgccgg cgcggtcggg ccttccagca ccatcttcgg
     1621 cgtcgaagcg gcgcacccgg ctagcaggac cccgaaccag atcagatgcc gaacgccact
     1681 tttggtttga ctgcgttgca cgtactctcc atcacatgcc atcgacacga ctggacatca
     1741 tcagcatcgg aacgctcagc cgcaaccggc tgtggaatga gaccgccgtc gtccgcaccg
     1801 ctcacgcgac gaccaccttg atccgcgcgg gcaaccgcaa cgtgctggtc gaccccggcc
     1861 tgccggccgc ggcgctgggc gcgagattat atgagcgcac gggaatgaag ccggagcaaa
     1921 tcgaaaccgt cttcctcacc aactcgcgcc ccgctcatcg cgcgggcctg tcgctgttca
     1981 ccaaggcgaa agtgctgatc cacgagatcg aacagcagtc ggcgcggcaa caattgcagc
     2041 agctgatcga ccaggcgccg gacgaagaca tcgaccgcgc gcacctgaag ggcgagctcg
     2101 acctgatcga aagtttcaaa cccgccgacg acgagatcgc cgatggcgtc gatctgttcc
     2161 ccctcttcgg ctacaccccc ggcacctgcg ggctgctggt cagcgccacg ctgacaacca
     2221 cattgatcgc cggcgacgcg gtgccgacgc tggatcattt tttagcagga caagtgctgc
     2281 cggacagctg ggacatcaag gcggcccatg agtcgatggc ggaggtgtac gagatcgcgg
     2341 atttgatcgt gccgggacat gacaacctgt tcatgaaccc gcgctcgcag ggggtgtgat
     2401 aggaaatgct tgtcgtgatt ggatgtgtgg gatattgtgt ggccatggcg agcaacctca
     2461 atatcgacga gcgattgctc tcgcaggccg tcaagacggg tggacataaa acgaagcgtg
     2521 aggcggtgaa ttcggcgctc gaggagtacg tccgccaccg ggaacgtcag aagatcacgg
     2581 agttgttcgg caccgttgaa tacgagccgg gacacgatcc gaagaacggc cggcggaagc
     2641 gatagatgcc ggtgctcgtt gatggttcgg tgtggtcttt agctcttcgc cgctctgcgc
     2701 cggatcaaac tcaccagagt gcgctcaatc gcctgatcgt cgcaggcgag gtgcgaatca
     2761 tcggacccat tcgccaggaa tgcttgtccg gaatcgcgaa cccagtcatt tccggcggct
     2821 gcgccatcgc ctgcgggcgt ttgcggatat tcctttactt cgggacgact acgaactcgc
     2881 cgccaccttc tacaacacgt gccggtcgcg tggcattcaa gggtcccaca ctgactttct
     2941 gatctgcgcg ctcgcagatc gagctcgtct gtctctatat acgaccgatg tcgatttcac
     3001 tcggtatgca gcgcacgccc caatcaagct gtttcgcccg taatgcgccg ttcaccgatc
     3061 aaactgcgca tcaaacgcaa tcttcgacgg cgcaaagtcc acccgcttca caaacgccgc
     3121 ggcttccttg gccccgtgct cgcggtccat ccggctgtcc tcccactcca cgctcagcgg
     3181 gccggcgtag ccgatgtcgt tcaacgcgac aatgatctcc tcaaaattca catcgccgtg
     3241 accgagcgag cggaagtccc agtatcgtcg cggatcggcg aacgttgtat gcccgccgaa
     3301 cacaccgacg gtgccgtccc cgtggccgaa ccacgcgtcc ttcatgtgca cgtggtagat
     3361 ccgattcgca aacgtccgga tgaacttcac ataatcgacg tgctggtaga tcaggtgcga
     3421 tggatcgaag ttgaagccga agcgcttgtg ccccttcacc gcgtcgatcg cgcgctgggc
     3481 ggtgacgata tcgaatgcga tttccgtcgg gtgaacttct aagccaaa
//
LOCUS       DATIMH010001034         8095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3420661, whole genome shotgun sequence.
ACCESSION   DATIMH010001034 DATIMH010000000
VERSION     DATIMH010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3420661"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3251
                     /locus_tag="VLI90_17970"
     CDS             <1..3251
                     /locus_tag="VLI90_17970"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV16154.1"
                     /translation="ATITNAVSGSTSGALTLVQNAAGGSNGGVGGGSTIYGNNGNGAS
                     SLTFSQTGMSSLTGYSNASGGNGNNGDGTPNVTGGNANASINLSTNTTVNANATANGG
                     AGNAVDNTQAGTGGAAIANAQGANSGTAAGASGTSVSASAGGGGGGYIGNGGGLGGNG
                     GNGGAATATATGFSAGPDSFNVTAFASGGGGNAGANGTNGSFVSGNGGNAIATATGTG
                     SGAAQVNVEAHASGGGGGPNSNGATAGHSGSATANATAITTGVGGYAIAQSFSAGSSG
                     TSTADARASGAAGSAIALARGVGTVPVDGDGPAEGRATIGQSLPVSSGVSAAAFAEGM
                     PLASDTANAWASSSNVQNVFGGNTANVHGLGFTELQVSSLGAGTSHTYSSVIEFTEDA
                     SALSSSSNLAVGLVGSALTGTGLGAGDSLRFRIVRAGSTLVDQTFSTNAAAQSYFANS
                     AFNLGVSNAGLGGGNLDLQFLFDLTSSHVGNGIGAQVVSGITNAAPGAVWNNANGGTW
                     VVGGNWTGNTVPDGPGLTVTFGNVISSPKTVTLDQSNTVGTISFPNTVGYTIAQGTPG
                     ASLIFDNGGAAATVLLSGFGGVTQTISAPVVLPAAGLNINLSLNSQTLALTGNISGSG
                     GINNIGSAGTLLLGGTNTFTGGVSASAGVIQISSDANLGAVPISPTTNMTFSGAELLA
                     TASLTTNANRNITLSNSPGPTSATLDGQGSGITLTIGGNISGSGPLTKGPSGNTVVLL
                     GNNTYAGTTTIGGGILQVGNGGTFGSLPAGNIADNATLVINRSDAVSMSNNISGTGAL
                     TKSGAGTLTLNAVNTYTGATTLIGGTLAVPAAASLASASITVAGGTTFNVDGSLASTA
                     AVFDGGTTNFAGNTSSATTLTRSLSSLSVSTGALAKVNLSSSAIVPEIFSPTTLTITG
                     TGKLDLGNNELKTAALLSDIRTLVNNGGIFTTHTSTTTGLGYASQGGGIIEVRYTLLG
                     DANLSGGVDVGDLGALATNYGTTTGAFWSQGDFNKDGAVNVGDLGALATNYGISLAAN
                     SEVGAPAAMIAAPTASASAAVPEPSSAVIILAAATALLPARRRRRSHVKAPI"
     gene            3251..3892
                     /locus_tag="VLI90_17975"
     CDS             3251..3892
                     /locus_tag="VLI90_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16155.1"
                     /translation="MATKKPLALRVLFIGNSFTARNDLPGLVALLADGAGNRLEHQLL
                     SIGGASLGTHWNKGEARAAIEHGRFDYVVLQEQSTLPIQNATRMHENVRLFDPVIRAA
                     GSKTALYMTWARKHAPQTQAVITAAYTSIAREIAATLIPAGIAWQRALRPPAKIVLHD
                     KDGSHPTLAGSFLAACVAYRALFRGRLPRDVNVDGLTEEQIKMLQSIAGTVTI"
     gene            complement(4127..4630)
                     /locus_tag="VLI90_17980"
     CDS             complement(4127..4630)
                     /locus_tag="VLI90_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16156.1"
                     /translation="MKTTLAIVAALLVCAAMFLLPHHKREAGKFQTGAFTLLDIFRDM
                     LDSLGDLQSPRAIVFAMLSVASLLLVFWPVLLVLIVRLTRGEPGPVTGPLLLMGGVAT
                     VAAAAVNFLMMVFSQTSIGFGGGHANARPTALIWVVPVIQVASGIAGAIAVFSPAALA
                     RLLAALV"
     gene            complement(4627..4947)
                     /locus_tag="VLI90_17985"
     CDS             complement(4627..4947)
                     /locus_tag="VLI90_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16157.1"
                     /translation="MATPSNQLKTCPHLQPIEQAILDAGAAIGAGMPSPYSGAGGTWY
                     PVAVTFDEPSLRRRLNLPAFVQFAEYDGRVAGSDASFYCTQCKCALMGLHPNYAQPDT
                     KRIA"
     gene            complement(5072..5959)
                     /locus_tag="VLI90_17990"
     CDS             complement(5072..5959)
                     /locus_tag="VLI90_17990"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HSV16158.1"
                     /translation="MTEWIWLNGETLTMADARLGIEDRGFQFADGVYEVIRIYNGKPF
                     TLIPHLQRLWNSAVDIQLTPPLTTDALAEQINRFIAHTGMREGFVYLQLTRGVAPRNH
                     LFPETIEPTLLFYVRPLQPPPLPGEGAGVALLAVPDDRWRRCWIKSIALLPNVLAKNA
                     AVAAGADEAAFIDGGGFVTECSTSNLFAVIEDKLVTHPVGTKVLPGITRAVLLEIAEQ
                     LGIELDERAIREDEAPRADELFITSTTREISWVARWNDIYIGQGRCGPTTMKLHRALQ
                     ERVRRETLSEIPTLATLAV"
     gene            6064..6921
                     /gene="ubiE"
                     /locus_tag="VLI90_17995"
     CDS             6064..6921
                     /gene="ubiE"
                     /locus_tag="VLI90_17995"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HSV16159.1"
                     /translation="MPTVEPSQAPINSPETAWDSQLLQNPHAVADKRRRVQRMFAAIA
                     PSYDLNNRLHSLWMDQRWRRKAVKLAGLKATDRVVDVACGTGDLSLQFYFECAVTNGR
                     VVTPKAHRVIGIDFTYEMLPLAREKFRAIAENWRHEYIAAPPTSNDLPFLNGDAQALP
                     LEDQSADVLSIAFGIRNVADPAAAIREFHRVLRPGGRLIILEFSLPTNALLRGLYNFY
                     FRKILPRTATLISGDRTGAYKYLPESVNTFIGREQMMGMMRDAGFADVEQHPMTFGVC
                     VCYRGVKRA"
     gene            complement(6964..7911)
                     /locus_tag="VLI90_18000"
     CDS             complement(6964..7911)
                     /locus_tag="VLI90_18000"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HSV16160.1"
                     /translation="MLRTSKRATGFTLVELLVVIGIIAVLIGILLPALSRARENAKRI
                     ACAAQLRQLGIATRNYAADNKDALPPINQDSGQVTYDANGGSVNLQRTVSFVCWGNTS
                     TLAAMQGAADEGPNFTGKANPIVGAGIGRLSALKYLGGDIRQTCTCPSNPIGPEGDIL
                     GASSESFYYYNLHWAVRNVNGQYYAGSFKKLSLYHKPKTSYTGVDLSTQPGPLAGPPV
                     PNCVADVDYALAIDPNFITNTASTLYGKATHTLGNSRAFNLLFVDGSVRTAIIPNNKT
                     FTATGSYRGEIDLVGYCESIIQNGKPHTGHEYRMAPIVQ"
BASE COUNT         1468 a         2599 c         2660 g         1368 t
ORIGIN      
        1 atgcgaccat caccaacgcc gtctccggtt cgacctcggg cgcgctgacg ctggtgcaga
       61 acgccgccgg cggcagtaac ggcggcgtcg gaggtggcag caccatctac ggcaacaacg
      121 gcaatggcgc ctcgagcttg accttttccc agaccggcat gtcgagcctg actggctact
      181 ccaacgcgtc cggtggcaac ggcaacaacg gcgacggcac accgaacgtc actggcggaa
      241 atgcgaatgc cagcatcaat ctctcgacca acacgacggt caacgccaac gcgaccgcca
      301 atggtggcgc cggcaatgcc gtggacaaca cgcaggccgg caccggtggc gcggcgatcg
      361 ccaatgcgca gggcgcgaac agcggcacgg ccgcgggggc cagcggtacg tccgtcagcg
      421 ccagcgccgg cggcggcggc ggtgggtaca tcggcaacgg cggtgggctc ggcggcaatg
      481 gcgggaacgg cggggccgcc accgcaactg ccaccggttt tagcgccggc ccagattcgt
      541 tcaacgtcac cgccttcgcc agcggcggtg gtggaaacgc cggcgccaac ggcaccaatg
      601 gcagcttcgt cagcggcaac gggggcaatg cgatcgcgac cgcgaccggt acgggcagcg
      661 gcgcggcgca ggtaaacgtt gaagctcacg cgagcggcgg cggcggtggc cccaacagca
      721 atggcgcgac cgcggggcac agcggcagcg cgacagccaa cgcgacggcc atcaccacgg
      781 gcgtcggcgg ttacgccatc gcgcagtcgt tcagcgccgg ttcaagcggc acgtccacgg
      841 ccgacgctcg cgcgagcggc gccgccggca gcgcgattgc gctcgcgcgt ggagtgggga
      901 cggttccggt ggatggagac ggtccagccg agggacgggc caccattggt cagtcgctgc
      961 ctgtgtcgag cggcgttagt gcggcggcct ttgccgaggg catgccactt gcctcggata
     1021 ccgccaacgc ctgggccagc agttccaacg tgcagaacgt cttcggcggc aacaccgcca
     1081 acgtgcacgg gctgggcttt actgaactgc aggtgtcctc gctgggcgcc ggcacgtcgc
     1141 acacctacag cagcgtgatc gagttcaccg aggacgcatc ggcgttgtcg tccagcagca
     1201 atctggccgt gggactggtc ggttcagcgc tgaccggcac gggtttggga gccggcgatt
     1261 cgctgcgctt ccgcatcgtc cgcgcgggct cgacgctggt cgatcagaca ttttcgacca
     1321 acgcggcggc gcagtcttac ttcgccaact cggccttcaa tctgggcgtt tcaaacgcgg
     1381 gcctgggcgg cgggaacctt gatcttcaat tcctgttcga cctgaccagc tcgcacgtgg
     1441 gtaatggcat cggcgcgcaa gtcgtcagcg gcatcaccaa cgccgcgccc ggcgcggtat
     1501 ggaacaacgc caacggcggg acctgggtgg tgggcggcaa ctggaccggc aacacggtgc
     1561 ccgacgggcc ggggttgacg gtcacgttcg gcaatgtgat cagcagtccc aagaccgtga
     1621 cgctcgatca aagcaacaca gttggcacga tctcgttccc caacaccgtt ggatacacga
     1681 tcgcgcaggg cacgcccggc gcttcgttga tcttcgacaa cggcggagcg gccgcgacgg
     1741 tgctgctgag cggattcggc ggcgtgacgc agacgatcag cgcgccggtc gtcctgcccg
     1801 ccgcgggatt gaacatcaac ctgagcttga acagccagac gctggcgctg accggcaaca
     1861 tcagcggcag cggcgggatc aacaacatcg gcagcgccgg caccttgctg ctcggtggaa
     1921 ccaacacctt taccggcggc gtgtcggcca gcgcgggggt gattcagatc tcctccgacg
     1981 ccaacctcgg cgcggttcca atctcgccca ccacgaatat gaccttcagc ggcgccgagt
     2041 tgctggccac cgccagcctg accaccaacg ccaaccgcaa catcaccctt agcaacagcc
     2101 ctggccccac cagcgccacg ctggatggtc aaggcagcgg cattacgctg acgatcggcg
     2161 gtaacattag cggcagcggt ccactgacca aggggcccag cggaaacacc gtcgtcctgc
     2221 tgggcaacaa cacctatgcc ggcacgacca cgatcggcgg tgggatcctt caggtgggta
     2281 acggcggcac gttcggttcg ctgccggccg gcaacatcgc cgacaatgcc acgctggtga
     2341 tcaaccgctc cgacgccgtg tcgatgtcca acaacatcag cggcaccggc gcgttgacca
     2401 agtccggtgc cggcacgctg actttgaacg ccgtcaacac ctacaccggc gcgacgaccc
     2461 tcatcggcgg cacgctggcc gtgccggcgg cggcttcgct ggccagcgcg tcgatcaccg
     2521 tcgccggcgg cactacgttc aacgttgatg gatcgctcgc atccaccgct gccgtattcg
     2581 atggcggcac gaccaacttc gccggcaaca cgtcgtcggc gaccaccctc acgcgatcgc
     2641 tcagcagcct gtcggtttcc accggcgcgc tggccaaggt gaacctgtcg tcgtcggcga
     2701 tcgtccccga gatcttcagc cccaccaccc tcaccatcac cggcaccggc aagcttgacc
     2761 tgggcaacaa tgaactgaag actgccgccc tgctgagcga catccgcacg ctggtgaaca
     2821 acggcggcat cttcaccacg cacaccagca caaccaccgg cctgggttac gccagccagg
     2881 gcggcggcat catcgaggtg cgctacaccc tgctgggcga cgcgaacctc agcggcggcg
     2941 tagacgtcgg cgacctgggc gcgctagcga ccaactatgg caccaccacc ggcgcgttct
     3001 ggtcgcaagg cgacttcaac aaggacggcg cggtgaacgt cggtgacctg ggcgcgctgg
     3061 cgacgaacta cggcatcagc ctggccgcga attctgaagt gggtgcgccc gcggcgatga
     3121 tcgcggcacc aacagcatcg gcatcggcgg cggttccgga gccgtcgtca gcggtgatca
     3181 tcctcgcagc cgcgaccgcc ctgctgccgg cgcgccggcg ccggcgttcg catgtgaagg
     3241 ccccgatctg atggcgacca agaaaccgct cgccctgcgc gtcctgttca tcggcaacag
     3301 cttcaccgcc cgcaacgatc tgccgggcct ggtggcgctg ctggccgacg gcgcgggcaa
     3361 tcgtctcgag catcaactgc tgagcatcgg cggcgcatcg cttggcaccc actggaacaa
     3421 aggcgaagcg cgagccgcca tcgagcacgg ccgattcgac tacgtcgtcc tgcaggaaca
     3481 aagcacgctg ccgatccaga acgccactcg gatgcacgag aacgtgcggc tgttcgaccc
     3541 ggtcatccgc gccgccggat ccaagaccgc gctgtacatg acgtgggcga gaaagcacgc
     3601 gccgcaaacg caggcagtca tcaccgcggc gtacacgtcg atcgccaggg agatcgccgc
     3661 cacgctgatc cccgcgggca tcgcatggca gcgcgccctg cggccgccgg cgaagatcgt
     3721 tctgcatgac aaagacggca gccatcccac gctcgccggc tcgttcctgg cggcgtgcgt
     3781 tgcgtaccgc gccctgtttc gcggccgctt gccgcgcgac gtaaacgtgg acgggttgac
     3841 ggaggagcag atcaaaatgc ttcagagcat cgccggcact gtcacgattt gattcggctc
     3901 gcgaaatgca accgccgaga cgccgagagc gccgaggcgg acgtcgagaa gaggttcaga
     3961 gaagactttg taaccacagg tttcacagat tacgcagatt gaagaagcgt atttcagaat
     4021 ctgtgtaatc tgtggaatct gtggtttaac cctcttctat tcttgtcttc tcgacgtccg
     4081 tctcggcgct ctcggcgtct cggcggttgc atgtcgcgag gcttcatcaa acgagggcag
     4141 ccagcaatcg cgccagcgcc gccggggaga acacggcgat cgcgccggcg atgccggagg
     4201 caacctgaat gactggcacg acccagatca gcgcggtggg ccgggcgttc gcgtggccgc
     4261 cgccgaagcc gatgctggtt tgggagaaga ccatcatgag gaagttgacc gccgccgcgg
     4321 cgacggtggc gacgccgccc atcagcagca gcggcccggt gaccggacca ggttcgccgc
     4381 gggtgaggcg gacgatcagc acgagcagga ccggccagaa gaccagcagc agcgatgcga
     4441 cgctgagcat ggcgaagacg atcgcccgtg gcgactgcag gtcgccgagc gaatcgagca
     4501 tgtcgcgaaa gatgtccagc agcgtgaacg cgccggtctg gaacttgccg gcctcgcgct
     4561 tgtgatgcgg caggaggaac attgccgcgc acacgagcag cgcggcgacg atggcgagcg
     4621 tcgtcttcac gcgatcctct tggtgtcggg ctgcgcgtaa ttcgggtgca ggcccatgag
     4681 cgcgcacttg cactgcgtgc agtaaaagct cgcgtcgctg ccggcgacgc ggccgtcgta
     4741 ttccgcgaac tgcacgaatg cggggaggtt gaggcggcga cgaagcgatg gctcgtcgaa
     4801 cgtgacggcg acggggtacc acgtgccgcc ggcgccgctg taggggctgg gcatgccggc
     4861 gccgatggct gcgccggcgt cgaggatcgc ctgctcgatg ggctggaggt gggggcaggt
     4921 ttttaattgg tttgatggcg ttgccataga gcgcgcgaga ttcagaatgc ggaaagaccg
     4981 agcgcggcgt ggtcgcttga gcgatcgtgt ctgtttcggg gcgggtgaag acatggtcgc
     5041 tcaagcgacc acgccggcgc agggtttttt atcaaaccgc gagtgtcgcg agtgttggga
     5101 tttcactcaa cgtctcgcgc cgcacgcgct cctgtagcgc gcggtgcagc ttcatcgtcg
     5161 tcggcccgca tcggccctgg ccgatgtaga tgtcgttcca cctcgcgacc cagctgatct
     5221 cgcgcgtcgt gctggtgatg aataactcat ccgcccgcgg cgcttcgtct tcgcggatcg
     5281 cgcgctcatc caattcgatg cccagctgct cggcgatctc gagcaacacc gcgcgggtga
     5341 tgccgggaag cactttcgtg ccgacgggat gcgtgaccag cttatcttcg atgacggcga
     5401 acagattgct cgtcgagcat tcggtcacaa accccccgcc gtcgatgaac gccgcttcat
     5461 ccgcccccgc ggcgacggcg gcatttttgg ccagcacgtt gggcagcagc gcgattgact
     5521 tgatccagca ccgccgccag cggtcatccg gcaccgccaa tagcgcgacg cccgcccctt
     5581 cgccgggcag cggcggcggt tgcagcggtc gcacgtagaa gagcaacgtc ggttcgatcg
     5641 tctcggggaa caggtgattg cgcggtgcga cgccgcgggt cagctgcagg tagacgaacc
     5701 cttcgcgcat gccggtgtgg gcgatgaagc ggttgatttg ctccgccagc gcatcggtcg
     5761 tcagcggcgg cgtcagttgg atatcgaccg cgctgttcca cagccgctgc aggtgcggga
     5821 tgagcgtgaa cggcttgccg ttgtagatgc ggatgacttc gtaaacgccg tcggcgaatt
     5881 gaaatccgcg atcttcgatg cccaggcggg catcagccat ggtgagcgtt tcgccgttga
     5941 gccaaatcca ttcggtcatc gcaaaacatc cttccgcgcc gttcacgctg tgcgtgttca
     6001 cgcgaattga aagcgggggg cgcgggcggt tcccacccga taccttataa tcgtccgcct
     6061 gcgatgccaa cagtcgaacc atcacaagcg ccgataaatt cgcccgaaac agcgtgggac
     6121 agccagctgt tacagaatcc ccacgccgtc gccgacaagc gccggcgggt gcaacgcatg
     6181 ttcgcggcga tcgcgcccag ctacgacttg aacaatcggc tgcatagctt gtggatggat
     6241 caacgctggc ggcggaaggc ggtgaagctg gcggggttga aggcgacgga tcgagtcgtc
     6301 gacgtcgcct gtggcaccgg ggatctttcc ttgcagtttt atttcgagtg cgcggtcacc
     6361 aacggtcgag tcgtcacgcc aaaggcgcac cgcgtgatcg gcatcgactt tacctatgag
     6421 atgttacctc tggctcgcga gaaattccga gcgatagcgg agaattggcg acacgagtac
     6481 atcgctgcgc ctccgacatc gaatgacctc ccctttctta atggcgatgc tcaggctctg
     6541 cccctggagg accaatccgc cgacgtcctc tccatcgcct tcggcatccg caacgtcgcg
     6601 gaccccgcgg cggccatccg ggaattccac cgcgtgctcc gccccggcgg gcggttgatc
     6661 atcctcgaat tctcgttgcc gacgaacgcc cttctgcgcg ggctctacaa cttctacttc
     6721 cgcaagatcc tcccgcggac ggcgacgctg atcagcggcg accgaaccgg cgcgtacaaa
     6781 tacctgccgg aaagcgtgaa cacgttcatc ggccgcgagc agatgatggg catgatgcgc
     6841 gacgcgggct tcgccgacgt cgagcagcac ccgatgacct tcggcgtgtg cgtgtgctat
     6901 cgcggggtga agcgcgcgta gttatgtgcg atggttcacg ccggcccggg cgcgcacgct
     6961 cgttcattgc acgatcggag ccatgcggta ttcatggccg gtgtgcggtt tgccgttttg
     7021 gatgatcgac tcgcaatacc cgaccagatc gatctcgccg cggtatgaac ccgtggctgt
     7081 gaaggttttg ttgttgggga taatggctgt cctgaccgag ccgtccacga agagcaggtt
     7141 gaacgcgcgg ctgttgccca gcgtgtgcgt tgccttcccg taaagcgtcg aggcggtgtt
     7201 ggtaatgaag ttcgggtcaa tcgccagcgc atagtcgaca tccgccacgc aattgggaac
     7261 aggtggtccg gcgagcgggc ccggttgtgt gctgagatcg acacccgtgt agctggtctt
     7321 gggcttgtga tagagcgaca gctttttgaa cgagccggcg tagtattgac cattcacgtt
     7381 tcggacggcc cagtggaggt tgtagtaata gaacgactcg ctgctggcgc cgaggatgtc
     7441 gccttcgggg ccgatcggat tggatgggca ggtgcaggtt tggcggatgt caccgccgag
     7501 gtactttaag gcggacaggc ggccgatgcc cgcgcccacg attggatttg ctttgccggt
     7561 gaagttggga ccttcgtcgg cggccccctg catggccgcc agggttgagg tgttacccca
     7621 gcacacgaac gataccgtcc gctgcaaatt gaccgacccg ccgttggcgt cgtaggtgac
     7681 ttgcccggag tcctggttga tgggcggcaa ggcgtccttg ttgtcggcgg cataatttcg
     7741 ggtcgcgatc ccgagctgcc gcagctgcgc ggcgcaggca atgcgcttgg cgttctcgcg
     7801 cgcgcggctg agtgccggca gcaggatgcc gatcagcacc gcgatgatgc cgatcaccac
     7861 gagcagttcc accagcgtaa aacccgtcgc acgttttgaa gtacggagca tgtcgaaccc
     7921 tcttttggac atcttgatcg cggcggcccg cgcggtgatg tgaatgggcg gcagccaacg
     7981 cgactcgtct attgtcgcac cgcgatcggc gcgagccgtt ggcaaatcga gcaattcggt
     8041 tgccgtttca ggaaggaaac caaaagaaac cgcccgcggg atgactcccg cgggc
//
LOCUS       DATIMH010001035         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2845548, whole genome shotgun sequence.
ACCESSION   DATIMH010001035 DATIMH010000000
VERSION     DATIMH010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2845548"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1436
                     /locus_tag="VLI90_18005"
     CDS             201..1436
                     /locus_tag="VLI90_18005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV16161.1"
                     /translation="MSDLQTDPPAATAAVSDHWPAVMAGFLGWTLDAFDFFLVVLCVP
                     AIAKTYGVDTPRITFTIALTLGMRPVGAFIFGLVADRYGRRRPLMINLVFYSTLSILS
                     GLAPTYLTFVICRALFGIGMGGEWGVGATLAMEKVPPRLRGLLSGLLQEGYATGNLLA
                     SAAYFFVFPRFGWRPLFVLGGLPALLALYIRFHVKESEVWQKTKHESWSHLGRGIAQH
                     WRLFLMLTLLMWMMNLSSHGTQDMFPTLLEKDWHFGPHAKAAVNAVSMLGAIAGGVVV
                     GLLSDRIGRRRAMVGSFICAILVIPLWAYAPNIALLVLGAFLIQFMVQGAWGVIPAHI
                     TELSPDSVRGFLPGFAYQCGVLLAGSIAWIQSLLSRHTTYANAMALTALTVFTAAAIV
                     IATGRERRGIRFGEDARAA"
BASE COUNT          233 a          556 c          503 g          326 t
ORIGIN      
        1 tttcccgcgg cagaacccgg gtcttcggcg tacctcagac accggcgtcg gtgacatcat
       61 ttggtttctc ttgcttgctt gcgtgagctt ccacattcca cagctcctgc tggacactga
      121 ttgatcgaca tccgagacta cccgcgcgcg agcgcgctgc gcgcgaaaga tagccgcgcc
      181 cgcgccgact ctgcaacttt atgagcgatc tgcagaccga tcctcccgct gcgaccgccg
      241 cggtcagcga ccactggccc gcggtgatgg ccggcttcct cggctggacg ctcgatgcct
      301 tcgacttctt cctcgtcgtg ctatgcgtgc ccgccatcgc caaaacctac ggcgtcgata
      361 cgccgcgcat cacgttcacc atcgcgctga cgctgggcat gcggccggtc ggcgccttca
      421 tcttcggcct cgtcgccgat cgttacggcc ggcgccggcc gctcatgatc aaccttgtct
      481 tctactcgac gctgtcgatc ctcagcggcc tcgcgccgac atatctcaca ttcgtcatct
      541 gccgcgcgct cttcggcatc ggcatgggcg gcgagtgggg cgtcggcgca acgctggcga
      601 tggagaaagt cccgccgcga cttcgcggcc tcttgtccgg tcttctccag gaaggctacg
      661 ccaccggcaa cctgctcgcc tccgcggcgt acttcttcgt cttcccccgc ttcggctggc
      721 ggccgctgtt cgtgctcggc gggctgccgg cgctgctcgc gctctacatc cgcttccacg
      781 tgaaggaatc cgaagtctgg cagaagacca agcacgaaag ctggtcgcac ctgggccgcg
      841 gcatcgcgca gcattggcga ttgttcctga tgctcacgct gctgatgtgg atgatgaacc
      901 tgtcgtcgca cggcacgcag gacatgttcc ccacgctgct ggaaaaagac tggcacttcg
      961 gcccgcacgc caaggcggcc gtcaacgcgg tttcgatgct cggcgcgatc gccggtggcg
     1021 tggttgtggg actgttgtcc gaccgcatcg gccgccgccg ggcgatggtc gggtcgttca
     1081 tctgcgcgat cctcgtcatc ccactctggg cgtacgcgcc gaacatcgcc ctgctcgtgc
     1141 tcggggcgtt cctcatccag ttcatggtgc aaggcgcctg gggcgtgatt cccgcgcaca
     1201 tcaccgagct gtcgccggat tccgtccgcg gctttctccc cggcttcgcg tatcagtgcg
     1261 gcgtattgct cgccggcagc atcgcgtgga tccaatcgct gctgagccgc catacgacct
     1321 atgcgaatgc gatggcgctg acggcgctca cagttttcac cgccgccgcg atcgtgatcg
     1381 ccaccggccg tgaacggcgc gggattaggt ttggcgagga tgcgcgcgcc gcgtgagcgg
     1441 gcgcggcgtg ctgaagaccg tcatcctgag cggaggcggt actccgccgg agtcgaagga
     1501 tctagccgag gcgtcggtga acggtcgctc gtgaaagacg gcgcagcagt cgctttcggt
     1561 tttgcagacc agtgaccgcg gcttgtccgt ctgcgaaacc gcaagcgact gctgcgcc
//
LOCUS       DATIMH010001036         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5450026, whole genome shotgun sequence.
ACCESSION   DATIMH010001036 DATIMH010000000
VERSION     DATIMH010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5450026"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VLI90_18010"
     CDS             <1..1124
                     /locus_tag="VLI90_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16162.1"
                     /translation="LLMLAVGLSPFFIFVNESPIDALYHAISASWTGVAVDFAVSRSG
                     NLNYSWGAYVAQIIQVGEVGGIFASMFAILISTRLWSKILCWGIWAYWSLFVFNGGRR
                     GELAFMVLPAIGLMFVKYQARAAMAFRKQSIKAYVVCGLFALFVLFCVQFQGFYRSIG
                     YSNADFSQFQLTKSQGNTMFSEGLIAWQTIPEQKDFFKDDIFGIKVPCAGMIRALPEQ
                     LWWFIIGPIPRALWTSKPIDPLWAWYNQTFTGSNNGTVGTTISHGVVGSWYFNYGAFG
                     CIEGALLMGWLMAVAERALQRSNGRPMGMLMSLAFGVWIFRIYRDFIFIDLYGLIIGG
                     VVLSALVYACRPFLGGAPAGQQHGFEPGMDHGSQPGYSV"
     gene            1310..2617
                     /locus_tag="VLI90_18015"
     CDS             1310..2617
                     /locus_tag="VLI90_18015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV16163.1"
                     /translation="MGFRWIVSQLGARQHYGVPRGFYYRDELRLLYTEAWCRWGRGLL
                     RRGPRPVRAFAGRSHPDIPNCLVRSFNFQNIYDQLTHRGDNASLQQQHLAYLRIGHWY
                     ASAVAKDLSRQKLDPDQDVFFGFNTGCLETFQMLRERGIISICDQIDPARFEEQLVFD
                     ESERWPGWEKQPGRIPEAYWQRMNEEWSAASMILVNSEWSRQALLKQGAAPEKIFIVP
                     PAYEPEKTHLPARRNFDRPLTVLWIGSVILRKGIQYLIEAAKLLQNEPQVQFIIAGPI
                     KISDEALATAPPNMKFLGRLTRDQTVDWYRQADVFILPTLSDGFAVTQVEAMAHALPV
                     IVTPNCGQVVTDGVDGLVIEPYSGQAIADAVTSLNADRQLLREMSYRALDKSAHFYLP
                     RQAQLLEEAVLSYRAGESIETSPDRLFASGARPGIRKPIAPRN"
     gene            2637..>3848
                     /locus_tag="VLI90_18020"
     CDS             2637..>3848
                     /locus_tag="VLI90_18020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV16164.1"
                     /translation="MPWIVSQHGARQHYATPRAFQRQGTLDTFYTDTWAGRAKSLLTH
                     FPGRAKAFAGRSSSELPPEKVVAFTLPTLCEIATRPLRVKDPSVEAAHLEWIREGQRF
                     CRRVNEHLSHRTLSPKSHAFYGCKSVCLETLQRVREQGIFTLVDQADPSRVEEKLVEQ
                     ERGKWPGWEAMTGKVPEAYYERCAAEWATASTVLVYSAWTKDAIVEQGVPAEKVMVVP
                     LAFETAVTEAPPPKPSDRPLTVLWLGTVMLRKGIPYLIEAARKVQDRNIRFVVAGQIL
                     ISEKAVASAPKNMQFIGRVLRDQAEAVYRSADVFVLPTISDSFALTQVEAMANGLPVI
                     ATKRCGEVVTDGVDGRIVPVADAESLAAAIAALDDDRALLAEMSTNARKKARTFSIDS
                     YGDQVRNEVLMR"
BASE COUNT          698 a         1163 c         1253 g          734 t
ORIGIN      
        1 tcctgttgat gctggcagtg ggcctgtcgc cgttcttcat cttcgtgaac gagtcgccga
       61 tcgacgcgct gtatcacgcg atttccgctt cgtggacggg cgtggcggtg gacttcgcgg
      121 tcagccgaag cggcaacttg aactacagct ggggtgctta cgtcgcgcag atcatccaag
      181 tcggtgaagt gggcggcatc ttcgcgtcga tgttcgccat tctgatttcg acccgcctct
      241 ggtcgaagat cctctgctgg ggaatctggg cgtattggtc gctgttcgtg ttcaacggtg
      301 gccgccgcgg tgaattggcc ttcatggtgc tgccggcgat cgggttgatg ttcgtgaaat
      361 accaggcgcg ggcggcgatg gcgttccgca agcagagcat caaggcatat gtcgtctgcg
      421 gcctctttgc gctgttcgtg ctgttctgtg tgcagttcca agggttctac cgcagcatcg
      481 gttattccaa tgccgacttc tcgcagttcc agctcaccaa gagccagggc aacacgatgt
      541 tcagcgaagg cctgattgcc tggcagacga ttccggagca gaaagatttt ttcaaggacg
      601 acatcttcgg catcaaggtg ccgtgcgccg gcatgatccg ggcgctgccg gaacaattgt
      661 ggtggttcat catcggcccc atcccccgcg cgctgtggac cagcaagccg atcgacccgc
      721 tgtgggcgtg gtacaaccag acgttcaccg gcagcaacaa cggcacggtg ggcacgacga
      781 tttcacacgg cgtggtgggc agctggtact tcaactacgg cgcgttcggc tgcatcgaag
      841 gcgcgctgct gatgggctgg ttgatggccg tcgccgaacg cgcgctgcag cgcagcaatg
      901 gccggcccat gggcatgctc atgagccttg ccttcggtgt ctggatcttc cgcatctatc
      961 gcgacttcat cttcatcgac ttgtacggtc tgatcatcgg cggtgtcgtg ctttccgcgc
     1021 tggtgtatgc gtgccggccg ttcctaggcg gcgcgccggc gggacaacag cacgggttcg
     1081 agccgggaat ggaccacgga tcgcaaccag gctacagcgt ctgatgcgcg cgcgggtgaa
     1141 tggctgtctt attccctctc ccgatactcc gggagaggct tagggtgagg gtgattctaa
     1201 gatgacgagc gcatctcgaa ttccaaatca ctctcacccc agcccactcc cggagtacca
     1261 ggagaggggg aaaagcgcgg acgtggttta tccaccgcgc gctcgataaa tgggcttccg
     1321 ctggatcgtc tcacaacttg gcgcgcggca gcactacggc gtgccgcgcg gtttctatta
     1381 ccgggacgag ctgcgcctgc tctacaccga agcctggtgt cgctggggcc gcgggttgct
     1441 tcgccgtgga ccgcggccgg ttcgcgcgtt cgccgggcga tcgcatccgg acattcccaa
     1501 ctgcctggtc cgttcgttca acttccaaaa catctacgat cagctgaccc accgcgggga
     1561 taacgcctcg ctccagcagc agcaccttgc atacctgcgc atcggccact ggtacgcgtc
     1621 ggccgtcgcc aaggatttga gccgccagaa actcgatccg gatcaggacg tattcttcgg
     1681 tttcaacacc ggctgtctcg aaacgttcca aatgttgcgc gagcgcggga tcatctcgat
     1741 ctgcgatcag atcgacccgg cgcgttttga ggagcagctg gtcttcgacg aatccgagcg
     1801 ctggcccggt tgggagaaac aacccgggcg gatccccgag gcgtactggc agcgcatgaa
     1861 cgaagaatgg tcggcggcgt cgatgatcct cgtgaattcc gagtggtcgc ggcaagcgct
     1921 gctcaagcaa ggcgcggccc ccgagaagat cttcatcgtt cccccggcgt atgaaccgga
     1981 aaaaacgcac ctgccggccc ggcggaattt cgaccggccg ctgaccgtgc tgtggatcgg
     2041 cagcgtcatc cttcgcaagg ggattcaata cctgatcgaa gcggccaagc tgctgcagaa
     2101 tgaaccgcag gttcaattca tcatcgccgg gccgatcaag atttccgatg aagcgttggc
     2161 gaccgcgccg ccgaacatga agttcctggg ccggttgacg cgcgaccaga ccgtcgattg
     2221 gtatcgccag gccgatgtgt tcatcctgcc gacgctgtcg gatggttttg ccgtcacgca
     2281 agtcgaggcg atggcgcacg cgctgcccgt catcgtcacg cccaactgcg gccaagtggt
     2341 gaccgacggc gtggacgggc ttgtcatcga gccatacagc ggccaggcaa ttgccgatgc
     2401 ggtcacctcg ctgaatgccg atcgccagct gctgcgcgag atgtcgtatc gtgcgctgga
     2461 caagagcgcg cacttctatc tgccgcggca ggcgcagctg ctggaagagg cggtgctgag
     2521 ttatcgcgcc ggcgagtcga tcgaaacatc gccggaccga ttgtttgcaa gcggcgcgcg
     2581 gcccggcata cgcaagccaa tcgcgccgcg aaactaaagg agtagaggaa acggctatgc
     2641 cgtggatcgt ttcacaacac ggtgcgcgac agcactacgc gacgccgcgc gcattccagc
     2701 gacaggggac gctggacacg ttctacaccg acacctgggc cggccgggcg aagtcgctgc
     2761 tgacgcattt ccccggccgc gccaaagcgt tcgccgggcg cagttcatct gagttgccgc
     2821 cggagaaagt cgtggccttc acgctgccca cgctgtgcga gatcgcgacg cgcccgctgc
     2881 gcgtgaagga cccgtcggtg gaagcggcac acctggaatg gatccgcgaa ggccagcgct
     2941 tctgccgccg cgtgaacgag cacctgtcgc atcgcacgct gtcgccgaaa tcgcacgcgt
     3001 tttacgggtg caaatcggtc tgcctggaaa cgctgcagcg ggtgcgcgag cagggcattt
     3061 tcacgctggt cgatcaggcc gacccgtcgc gggtcgagga aaaactggtc gagcaggagc
     3121 gcggcaaatg gcccggctgg gaagcgatga ccggcaaggt ccccgaggcg tactacgaac
     3181 gctgcgccgc cgagtgggcc accgcttcga ccgtgctggt ctattccgcg tggaccaagg
     3241 acgcgatcgt cgaacaaggc gtgccggcgg aaaaggtgat ggtcgtgccg ctggcgttcg
     3301 agaccgcggt gaccgaagcc cctcccccga agccaagtga tcgcccgctg accgtgctgt
     3361 ggctcggcac cgtgatgctg cggaagggta ttccctacct gatcgaagcc gcgcgaaagg
     3421 tgcaggaccg gaacatccgc ttcgtcgtcg cggggcagat tctcatttcc gaaaaagccg
     3481 tcgcgtcggc cccgaagaac atgcagttca tcggccgcgt gctgcgcgac caggcggagg
     3541 cggtgtatcg ctccgccgac gtcttcgtgc tgccaacgat ctccgacagc ttcgccctga
     3601 cgcaggtaga agcgatggcg aacggcctgc cggtgatcgc gacgaagcgc tgcggcgaag
     3661 tggtgacgga cggcgtcgat gggcgcatcg tgccggttgc cgatgctgag tctctggccg
     3721 cagcgattgc ggcgttggac gacgatcgag cgctgttggc ggagatgtcg acgaatgcgc
     3781 gcaagaaggc gcggacgttc tcgattgatt cgtatgggga tcaggtgcga aatgaagtgc
     3841 tgatgcgc
//
LOCUS       DATIMH010001037         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3995995, whole genome shotgun sequence.
ACCESSION   DATIMH010001037 DATIMH010000000
VERSION     DATIMH010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3995995"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VLI90_18025"
     CDS             <1..458
                     /locus_tag="VLI90_18025"
                     /inference="COORDINATES: protein motif:HMM:NF037003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16165.1"
                     /translation="VGWGPSLHPSKLTPDEQYTHISLWSLLSAPLLIGCDMTQLDQFT
                     LNLLTNDEVLAVNQDPLGKQAAQVYHDDEDVEVWAKDMSDGTKAVGLFNKGETPTKVT
                     ARWSDLGVSGSQPVRDLWRQKDLGSFDGQYEVEVPRHGAVMIKVGTAKP"
     gene            798..1994
                     /locus_tag="VLI90_18030"
     CDS             798..1994
                     /locus_tag="VLI90_18030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSV16166.1"
                     /translation="MAHATDDPPPTTAPSTQASPQVAALLQRIDAAYSHLQSAQFGGH
                     LIGHYDIAGQKSDRDVAFTSSYAAPNRFRHETKSDVVIVSTGEKVYWFEPSRNSYGMN
                     DAPTGRAALAEWPHVVAVMLPYQNPSLLLALTPSASAGLSDLARDMTRQPDTTIDGVA
                     YPTLAFDLPTKEHVTMLVDPSTSLLRQVRFDSRQMLEEKRGATDVKQAEFIVDYTSAS
                     PVAIAADDARFKWTPPAGAVLASADTGPGAGDAGSASAMIGKPAADFSLKGLDDKPVT
                     MASLKGSVVVLDFWATWCGPCVAALPHLDALYREQSPHGLKLFAVDFMEDADTVKPFV
                     QEKKLSMPVLLDTAGDVGKNYGAADGIPVTVIIGKDGLVKKVFLGASDEIEQQIKDIV
                     VKEMGT"
     gene            2127..>2402
                     /locus_tag="VLI90_18035"
     CDS             2127..>2402
                     /locus_tag="VLI90_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16167.1"
                     /translation="MTKPSDRSKRSQTAIVHVEPLESRQLLSLTVEVRDAAGGHSATV
                     NAVGQTLNLQVWANVTGANAVAAQDGLLDVIGSFITTGIDFGPVKGNL"
BASE COUNT          488 a          704 c          773 g          437 t
ORIGIN      
        1 aggtcggctg ggggccgtcg ctgcacccgt ccaagctgac gccggacgag cagtacacgc
       61 acatttcgct gtggtcactg ctgtcggcgc cgctgctgat cgggtgcgac atgacgcagc
      121 tggaccagtt cacgctgaac ctgctgacga acgacgaagt gctggcggtg aaccaagacc
      181 cgctggggaa gcaggcggcg caggtttatc acgatgacga ggacgtggag gtgtgggcga
      241 aggacatgtc cgacggcacc aaagcggttg gcttgttcaa caagggcgag acgccgacca
      301 aggtgacagc gcgttggtca gatctgggcg tgagcggatc gcaacccgtg cgcgacctgt
      361 ggcggcagaa ggacctcgga tcgttcgacg ggcaatacga agtagaagtc ccgcggcacg
      421 gggcggtgat gatcaaggtg gggacggcga agccttgagc gccgaatgcg aacgcgaaga
      481 cgcgaaggca cgaagacaga cgcgaagcag atcggaactg agggattaac tgagatgcga
      541 gagtcgcacg cacgggccgg aggctcctgt tcacgattac gatttgaaga acgttcaacg
      601 tgacacgggc ctccggcccg tgcatgcgat gctgaaacta ttagcgcctt taccttccaa
      661 ttaatccctt cgcgtctgtc ttcgcgattt cgcgtcttcg cgatcgtatt aattctttcc
      721 ccgcggcgcg cggctatcat tgttccatgc tgcgaatctc actcccacga ctgctgccac
      781 tgctcatgat tgctgcagtg gcgcacgcta ccgatgaccc accaccgacg accgcccctt
      841 cgactcaagc ctcgccgcag gtcgcggcgt tgttgcagcg gatcgacgcc gcttattcac
      901 acctgcagtc cgcgcagttc ggcggtcatc tgatcggtca ctacgacatc gccggtcaaa
      961 agtccgatcg ggacgtcgcg ttcaccagct cgtatgccgc ccccaatcgt tttcggcacg
     1021 aaaccaaatc cgacgtcgtc atcgtcagca ccggtgaaaa ggtgtactgg ttcgagccga
     1081 gccgcaattc ctacggcatg aacgatgccc cgaccggccg agcggcgctg gcggagtggc
     1141 cgcacgtggt tgcggtgatg ctgccgtatc agaacccgtc gctgctgctg gcgttgacgc
     1201 cgagcgcatc cgcggggttg agcgacttgg cccgggacat gacgcgccag cccgacacga
     1261 cgatcgacgg cgtcgcgtat ccgacgctgg ccttcgatct gccgacgaag gagcacgtga
     1321 cgatgctggt cgatccatcg acgtcgctgc tgcgccaagt gcgtttcgat tcgcggcaga
     1381 tgctcgaaga aaagcgcggc gcgacagacg tgaagcaggc ggagttcatc gtcgattaca
     1441 ccagcgcatc gccggtggcc atcgccgcgg atgatgcccg cttcaagtgg accccgccgg
     1501 cgggcgcggt gctggccagc gccgacaccg gacctggcgc gggtgatgcg ggcagcgcgt
     1561 ctgccatgat cggcaagccg gcggcggact tctcgctcaa ggggctcgat gacaagcccg
     1621 tgacaatggc gtcgctgaag ggatcggtcg tcgtgctgga tttctgggcc acatggtgcg
     1681 gcccgtgcgt cgcggcgttg ccgcatctgg acgcgttgta ccgcgagcaa tcaccgcacg
     1741 ggttgaaatt gtttgccgtc gatttcatgg aggatgccga cacggtgaag ccgttcgtgc
     1801 aggaaaagaa gctttccatg cccgtgctgc tggacaccgc gggcgacgtc ggcaagaact
     1861 acggggcggc ggacggcatc ccggtgaccg tcatcatcgg caaggacggc ctggtcaaga
     1921 aagtgttcct cggcgccagc gatgagatcg aacaacagat caaggacatc gtcgtcaagg
     1981 aaatgggaac ctgaatttgg aaccacagat gcaaacagat gcacacagat aagaccagaa
     2041 ttgtcttgat cagtgtgcat cagtgtgcat ctgtggttcc atttcattgt tctatttggg
     2101 ccgggttgca agtaaggaca atcgctgtga ccaaaccatc tgaccgtagt aaacgcagtc
     2161 aaacggcaat cgttcatgtc gagccgctgg aatcacggca actcttgtcg ctaacggtcg
     2221 aagtgcgcga cgccgcaggc ggccactcgg ccacggtgaa cgcggtgggg cagacgttga
     2281 acctgcaggt gtgggccaac gttaccggcg ccaacgccgt ggcggcgcag gatgggctgc
     2341 tcgacgtgat cggatcattc atcaccaccg gcatcgactt cggacccgtg aagggaaacc
     2401 tg
//
LOCUS       DATIMH010001038         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_827214, whole genome shotgun sequence.
ACCESSION   DATIMH010001038 DATIMH010000000
VERSION     DATIMH010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827214"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VLI90_18040"
     CDS             <1..225
                     /locus_tag="VLI90_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16168.1"
                     /translation="AQRTGGVIAAYRLLVEHLSASEAYTEMRRFGHDPHDNPHLVEYL
                     NQHMGDIARLLVEKGVIDRVPEPMPDLGGL"
     gene            457..708
                     /locus_tag="VLI90_18045"
     CDS             457..708
                     /locus_tag="VLI90_18045"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HSV16169.1"
                     /translation="MRRSTLSQWGNSLGLRIPQEAAAQLQLKAGEQVSVEVGQDAIII
                     RPARRRRRKWALSALLKGVTPVKVGGEFGWGEPVGKERL"
     gene            705..1076
                     /locus_tag="VLI90_18050"
     CDS             705..1076
                     /locus_tag="VLI90_18050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HSV16170.1"
                     /translation="MSKRTYVPERGDVIHVNLGGTAGREFDGPHFALVISHGEFQRKT
                     GLCVVLPTTSKNHPELAALAVKLPPLAGLNPDGWVLIHRVRTVDFRERGAERAAQLQP
                     DDPRHQQYLGDLIDRLFGIVD"
     gene            1139..2941
                     /locus_tag="VLI90_18055"
     CDS             1139..2941
                     /locus_tag="VLI90_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16171.1"
                     /translation="MTHAAPELLRRAPSSDDVARAIRDLLLVLLAGLFVYCWRLGDSP
                     LDRTEPHRALVAHQMASGGEWLIPHLFGEVYLRKPPLIYWVQAATEKLVGHGNEFVWR
                     LPSAVGSALLAVALAWWAGRWFGSFARLPAGFACLALIAVWEQDRGADIDALNTVASV
                     LAAMVILEILFGREPAAPIAPAESRTIPVRSSFSFAWTFALAITTWATLMLKGPGGFP
                     PILGAILGPAIVLRTWRRLRQPSLWLGLLLGIAGFVTYGVLVKLQIHRRGLVTDKAGL
                     KEAMQRMLLHDVHQIVPALSAPFIALLFAIPVSLAIPFALGLVWPEPKDNPRRQRVLA
                     IVGAIAAALVIWVIAGNGNPRYEYVALPLLAPLVGAVAVGWREGQFSPATVERLRGIM
                     ISIGGLWVGVQLAMTRAIWLRATDRASLTIAAGLSLILAAAAAFLWARSRYRLAAPLL
                     AVVILLLTVPLSDRKNIERRRKSAQSTAQQLRSIVGDGTTVGVGSMVRDMPELFYYAG
                     VQVHAYGEFGLPALAADAGARWVVLSENKRFPELSTLQQQVIDAFPRGVTKLNMPDPA
                     DHVYVAWYDPPAGASRAVHYTSNPAADTSAGSDE"
     gene            3113..3931
                     /locus_tag="VLI90_18060"
     CDS             3113..3931
                     /locus_tag="VLI90_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16172.1"
                     /translation="MKADRRHQLASNALARELEGLPDKLKRWGSTALTVFLVAAAVGM
                     LIRWRMQSAEQSRLAVMNELANARAYVDQLRNPLLAQRPPDFVASARQELSTKANESI
                     STVLNTSDDTKAKADALVTRGDLYWQLANFPELPGASTQPSLRAPESSDEYLKKSFDA
                     YDEVLKNTQFAKQHDAVVSAHFGLAAIAENRRDWDEAKRHLDLIANDPDAQPALVNQA
                     KLQLEKELPQMQTPLYVMPAATQPAPTQPAATTQPTTQQASATTEPTPTTQPVH"
BASE COUNT          678 a         1276 c         1289 g          702 t
ORIGIN      
        1 gcccagcgca ccggcggcgt aattgcggcg tatcggctgc tggtcgagca cctttccgcg
       61 agcgaggcgt acacggagat gcgacgcttc ggtcacgacc cgcatgacaa tccgcatttg
      121 gtggaatatc tgaatcagca catgggcgat atcgcgcgat tgctggtgga gaagggcgtg
      181 atcgaccggg ttcccgagcc gatgccggac ctgggtgggc tgtaactgtt tcggtcgccc
      241 gcgacgccgg gtccaacgcg ctgacgtgac ccgagcgaag caaatacccg ctcgcagtcg
      301 ctcctgctcc agccggtgga caatggcttc cacggatgat atctttttga tatccaagga
      361 gattgcatgc cagtcgtcgc gcgtgtacat gccggcaaac gttcgaatgg gcgccgcaaa
      421 cccgccccgc gacgttctcc ggcgcagccg ctggcgatgc gcaggagcac cctgtcgcag
      481 tggggaaaca gcctcggcct gcgcattccg caggaggccg ccgcacagct tcaactgaag
      541 gcgggggagc aagtgtccgt ggaggtcggg caggacgcga tcatcattcg ccccgcgcgc
      601 cggcgccggc ggaaatgggc gctgtccgcg ctgctcaaag gcgttacacc ggtgaaagtc
      661 ggtggcgaat tcggttgggg cgagccggtc ggcaaggaac ggctttgagc aagcgcacct
      721 atgtgccgga acgcggtgat gtcattcacg tcaacctcgg cggcacggct ggccgcgagt
      781 tcgacgggcc gcacttcgca ttggtgatca gccacggtga gttccagcgc aagacaggct
      841 tgtgcgttgt gctgccgacg acgtcgaaga accatcccga acttgccgcg ttagcggtca
      901 agcttccacc actggctggg ctgaatcccg atggttgggt gctgattcac cgcgtgcgga
      961 cggttgactt ccgcgagcgc ggcgcggaac gagccgcaca gctgcaaccc gatgatccgc
     1021 gccatcaaca atatctcggc gacctgattg accgcctgtt cggcatcgtc gattgagccg
     1081 ctcgggatca cgtcaacgtc ttgccgattg tgcgccaaag ctcaagatcg aatccccgat
     1141 gactcatgcc gctcccgagt tgcttcgccg cgcgccctcg tccgatgacg tcgcgcgagc
     1201 aatccgcgac ctgctgctgg tcctcctcgc cgggctgttc gtctactgct ggcggctcgg
     1261 tgattcgccg ctggatcgga ccgagccgca tcgcgcgctc gtggcgcatc agatggccag
     1321 cggcggcgag tggttgatcc cgcatttgtt cggcgaggtt tatctccgca agccgccgct
     1381 gatttactgg gtgcaggcgg cgactgaaaa gttggttggt cacggcaacg aattcgtctg
     1441 gagattgccg tcggcggtcg gttcggcgct gctggcggtc gcgctggcgt ggtgggcggg
     1501 gcggtggttc gggtcgttcg cgcgattgcc ggcggggttc gcctgtctgg cgctgatcgc
     1561 cgtgtgggag caggaccgcg gagcggatat cgatgcgctg aacaccgtcg ccagcgtgct
     1621 ggcggcgatg gtgatcctgg agattctctt cggacgagaa ccagccgcgc cgatcgcgcc
     1681 agcggagagc cggaccatcc cggtccggtc ctcgttcagc ttcgcctgga ccttcgcgct
     1741 tgcgatcacg acctgggcca cgctgatgct gaaaggcccc ggcgggttcc cgccgatcct
     1801 gggcgcgatc ctcggtcccg cgatcgttct tcgcacctgg cgtcgcctgc gccagccgag
     1861 cctttggctc ggcctgctgc tcggcatcgc cggcttcgtc acgtatggcg tgctggtcaa
     1921 actccaaatc caccgccgcg ggctggtgac ggacaaagcc gggctcaaag aagccatgca
     1981 gcgcatgctg ctgcacgatg tgcatcagat tgtccccgcg ttgtcggcgc cgttcatcgc
     2041 gctgctgttc gcaattccag tgtcgctcgc catccccttc gcgcttgggc tggtgtggcc
     2101 agagccgaag gacaatccgc gccgccagcg cgtgctggcg atcgtcggcg ccattgcggc
     2161 ggcgctggtc atctgggtga tcgccggcaa cggcaacccg cggtacgagt acgtcgccct
     2221 gccgctgctg gcgccgctgg tcggcgcggt cgccgtcgga tggcgcgagg gacaattttc
     2281 gccggcgacc gtcgagcgac tccgcggcat catgatcagc atcggcgggc tgtgggtcgg
     2341 cgtgcagttg gcgatgacgc gcgcgatctg gctgcgcgcg accgatcgcg cgtcacttac
     2401 gatcgccgcg ggactgtcgc tgatcctcgc cgccgccgcg gcgtttctat gggctcgctc
     2461 gcgctatcga ctcgccgctc cgctgctggc ggtggtcatc ctgctgctga cggtgccgct
     2521 gagcgatcgc aaaaacattg aacggcggcg gaagtcggcg caatcgacgg ctcaacagct
     2581 gcggtcaatc gtcggtgacg gcacgaccgt cggcgtcggc agcatggtgc gcgacatgcc
     2641 cgaactgttc tattacgccg gtgtccaagt gcacgcctat ggcgagttcg gcctgcccgc
     2701 actggccgcc gatgccgggg cgcgatgggt cgtgctcagc gaaaacaagc ggttccctga
     2761 actgtctact ctgcagcagc aggtaattga tgcgttcccc cgcggcgtga cgaagctgaa
     2821 catgccggac ccggccgacc acgtctacgt cgcctggtac gatcccccgg cgggcgcatc
     2881 gcgcgcagtg cactacacct caaaccccgc tgccgacacg tcggcgggca gtgacgaata
     2941 aatagtcatg gaactcccac cgcgatattc gtgcgcgcag gcgagacatt tcgagacatt
     3001 ttccgaaaaa tgtctcgcgc gcgccttaaa tgtcccgctt ttgcaaaagt gagcgccggg
     3061 acatcggccg cgtttgcttc gcgttggatc ggtcattaca ctcccgcgtc ccatgaaagc
     3121 ggatcggcgg catcaattgg catccaacgc gctggcgcgc gagctcgagg gtttacccga
     3181 caagctcaaa cgctggggca gcaccgcgct gacggtcttc ctggtcgcgg cggccgtcgg
     3241 gatgctcatt cgctggcgca tgcagtcggc cgagcagagc cggctggcgg tgatgaacga
     3301 gctggccaac gcccgggcgt atgtcgatca actgcggaat ccgttgctgg ctcagcgtcc
     3361 gccggacttc gtcgcctccg cccgccagga actatccacc aaggcgaacg agtcgatctc
     3421 gacagtgctg aacacctccg acgataccaa ggccaaggcc gacgcactgg tcacccgcgg
     3481 cgatctatat tggcaactgg cgaacttccc cgagcttcct ggggcatcca cgcaaccgag
     3541 cctgcgggcg cccgagtcga gcgatgaata tctgaaaaaa tcgttcgacg cgtatgacga
     3601 agtgctgaag aacacgcagt tcgccaagca gcacgatgcg gtggtgagcg cgcacttcgg
     3661 cctggccgcc atcgcggaaa accggcgcga ctgggacgaa gccaagcggc atctcgatct
     3721 gatcgccaac gatcccgatg cccagccggc gctggtgaac caggcgaagc tgcagttgga
     3781 aaaggagctg ccgcagatgc agacgccgct gtacgtgatg cccgcggcga cgcaacccgc
     3841 gcccacgcaa cccgcggcga cgacgcaacc aacaacacag caagcatcgg cgacaaccga
     3901 accaacgccc acaacgcaac cggtccactg aaagtccgcg cggcg
//
LOCUS       DATIMH010001039         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3136196, whole genome shotgun sequence.
ACCESSION   DATIMH010001039 DATIMH010000000
VERSION     DATIMH010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3136196"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VLI90_18065"
     CDS             complement(<1..688)
                     /locus_tag="VLI90_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16173.1"
                     /translation="MRLVSAGASGKSAGAKYTITHMARVAPRVPEMNDVLCEGCGYVL
                     NGLPPDGRCPECGKPIEESIGRNRIAPPWESAPKSAGFLRTTLDVIFRPTQFYQTLAV
                     RGSVESARRFAQLHWWISAGLFGLTCATHTLWYGFIMPSVGSASPGPRIIAFYVAAVV
                     VLTVTTYFALDGITRLAARLTNWEATYRGIRLPYDIVLRGMYYHAAHYIPVALVAAVT
                     VIGYTVLVSTH"
     gene            687..1298
                     /locus_tag="VLI90_18070"
     CDS             687..1298
                     /locus_tag="VLI90_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16174.1"
                     /translation="MRRSTIRFVQSTAIAAAVCVSLLGCGKHSDSSATSDSNPPTATT
                     TLAGNPTSAPSTQPAASLEIDGKPVAFPPAKLVITKKAGGLEAILCSDDPPNAIDPGY
                     TGNSFMIEMKLDVDDPADLPTAVWEYKTEKQSPQDSTTGIFLNGARRQMQPSDVRITF
                     EKQGDRMVAMVNGKFLLFDTQDVAAPVQVVDVKGKLDAAVREQ"
     gene            1483..1959
                     /gene="smpB"
                     /locus_tag="VLI90_18075"
     CDS             1483..1959
                     /gene="smpB"
                     /locus_tag="VLI90_18075"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HSV16175.1"
                     /translation="MSAQKNANDAPRITNRRALHDYFITHKIECGMVLVGSEVKSLRA
                     GLAQLHESFARIENGNLILHNCHIDPYARAAIVYNHDPRRERKLLVHKRELKKLASET
                     DVKGTTLIPLSIYFKDGRAKLELGVAKGKQHFDKRATIKKKEMDRELRRQTMTHRH"
     gene            2016..2816
                     /locus_tag="VLI90_18080"
     CDS             2016..2816
                     /locus_tag="VLI90_18080"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HSV16176.1"
                     /translation="MTSASPFARLLSLALCIALFALPSRSNAADDAAAPRIRIVLAGD
                     STTAVNSGWGPGFMKCLTPDVECINMARSGRSSRSYIAEGWWKKCLALKPDYVLIQFG
                     HNDQPGHGADRETDPETTYRQYMNQYVDDARAAGVKPVLITSLSRRQWGDDGHIHSTL
                     VPNVEVVKQIAAAKNVPLIDLHARSIELYEKLGKEGCNELSPVKNATTTRSSKDDEAI
                     ADNASAAKVYDGTHLNAKGATVIGKIVAEELAKAVPDLAPYIKRPLAK"
     gene            2934..>3235
                     /locus_tag="VLI90_18085"
     CDS             2934..>3235
                     /locus_tag="VLI90_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16177.1"
                     /translation="MPSPLPLSIASPGPLLAAGALLLVLMIIAFIRRLALARASQLLL
                     AMGLILLTLAAGEPQWWRVATSSVVVMVDLSPSTRGATFRHRDALIARVHQLLGNQP"
BASE COUNT          644 a         1120 c          958 g          513 t
ORIGIN      
        1 cgtgcgtcga gaccaggacc gtgtaaccga tgacggtcac cgccgcgacc agcgcgacgg
       61 gaatgtagtg ggccgcgtgg taatacatgc cgcgcaggac gatgtcgtac ggcaggcgga
      121 tgccgcgata cgtcgcctcc caattggtca gccgagctgc caaccgagtg atgccatcga
      181 gcgcgaagta tgtcgtcacc gtcagcacga cgacggcggc aacataaaac gcgatgatcc
      241 gcggccccgg tgacgccgag cccaccgacg gcatgatgaa cccataccaa agcgtgtgcg
      301 tcgcgcacgt caatccaaat aaaccggcgg aaatccacca gtgcagctgc gcgaatcgcc
      361 gggcgctctc gaccgagccg cgcacggcga gcgtctgata aaactgcgtg ggccgaaaga
      421 tcacgtccag cgtcgtccgc aaaaaaccag cgctcttcgg cgccgattcc cacggcgggg
      481 cgatgcggtt gcgtccgata ctttcctcga tcggtttccc gcactcgggg cagcgtccgt
      541 cgggcggcag gccattcagc acgtaaccgc acccttcgca caggacatcg ttcatctccg
      601 gaactcgcgg ggcgacgcgg gccatatgcg ttatggtata cttcgccccg gcggatttcc
      661 cactcgcccc cgcggagacg aggcgcatgc gtcgatcgac aatccggttt gtccaatcca
      721 ccgccatcgc ggccgcggtg tgcgtgtcgc tccttggctg cggcaagcat tccgactcgt
      781 ccgcgacgag cgattcgaat ccgccaaccg ccacgacgac cctcgccggg aatccgacga
      841 gcgcgccctc cactcaaccc gccgcctcgc tggagatcga cggcaagccc gtcgcgttcc
      901 ccccggcgaa gctggtcatc accaaaaaag ccggcggcct cgaggccatc ctctgcagcg
      961 acgatccccc caacgcgatc gatcccggtt acaccggcaa cagcttcatg atcgagatga
     1021 agctcgacgt ggacgacccc gccgacctgc cgaccgcggt gtgggaatac aagaccgaaa
     1081 aacaatcgcc gcaggattcg acgaccggca ttttcctgaa cggcgcccgc cggcagatgc
     1141 agccatcgga tgttcgaatc accttcgaaa agcagggcga tcgcatggtc gccatggtga
     1201 atggaaagtt cctgctgttc gatacccagg acgtcgccgc cccggtgcaa gtggtggatg
     1261 taaagggcaa actggacgcg gccgtgcggg agcagtgaag cacgacgccc atcgacattc
     1321 atcgccccct tgatagccgc gggcgacagc ccgcgggtgc gttcgagaac aaggcgggta
     1381 tcgacttctc aacacccgct ggctttcgcc cgcggctatt accggcagcg atgtgcggtg
     1441 cgccttcccc cttgccgcgt gcgcttctat cattctccag cgatgtccgc tcagaagaac
     1501 gccaacgacg ccccgcgcat caccaatcgg cgggcgctgc acgattactt catcacgcac
     1561 aagatcgagt gcggcatggt gctcgtcggc agcgaagtga aaagcctgcg ggccggcctg
     1621 gcccagctgc acgaatcctt cgcgcgcatc gagaacggca acctgatcct gcacaactgc
     1681 cacatcgatc cctacgcacg ggcggccatc gtttacaacc acgacccccg gcgtgagcgc
     1741 aagctgctcg tgcacaagcg cgagctcaaa aagctcgcca gcgaaaccga cgtcaaaggc
     1801 accacgctca tcccgctgtc gatctatttc aaagatggcc gagccaagct ggaacttggc
     1861 gtcgccaagg gcaagcagca cttcgacaag cgtgcgacga tcaagaaaaa ggaaatggac
     1921 cgcgagctgc gccgccagac gatgacgcac agacactgac cacgccgggt tcggagggta
     1981 ctcccgacga cccggatgct ttcccgttaa accacatgac ttcagcatcc ccattcgctc
     2041 gactactatc cctcgccctc tgcatcgcgc tttttgcact gccctcgcgt tccaacgccg
     2101 ccgacgacgc ggctgcgcca aggatccgca tcgtcctcgc cggcgattcc accaccgccg
     2161 tcaacagcgg ctggggcccg gggttcatga agtgccttac ccccgatgtc gaatgcatca
     2221 acatggcccg cagcggacgc agctccagga gctacatcgc cgaaggctgg tggaagaaat
     2281 gcctggccct gaagcccgac tacgttctga tccagttcgg ccacaacgac caacccggcc
     2341 acggcgccga tcgcgaaacc gatcccgaaa cgacgtaccg ccaatacatg aaccagtacg
     2401 tggatgacgc ccgcgccgcc ggcgtcaagc cggtgctcat cacatcgctc agccgccggc
     2461 agtggggcga tgatgggcac atccattcca cgctcgtgcc gaacgtggaa gtcgtcaagc
     2521 agattgccgc cgcgaagaac gtcccgctga tcgacctgca cgcgcggtcg atcgagctgt
     2581 acgagaagct cggcaaggaa ggctgcaatg agctctcacc cgtgaagaac gcgacgacga
     2641 ccaggtcgtc caaggacgat gaagccatcg ccgacaacgc gtcggcggcc aaggtgtacg
     2701 atggcacgca cctgaacgcg aagggcgcca ccgtgatcgg aaaaatcgtc gccgaagaac
     2761 tcgccaaggc ggtaccggac ctggcgccgt acatcaagcg gccgctggcg aagtgacgct
     2821 caccaccgcg cgacccgctt aagcgggtcg gtctgccggc tcaggcctcg cgggtttagc
     2881 tatgagcgga tagattcggt ctggcgcgct cgcggcgccg ccgcgcgatc tcgatgcctt
     2941 cgccactccc gctttccatc gcctcgcccg ggccgctgct ggcggcgggg gcgctgctcc
     3001 tcgttctcat gatcatcgcg ttcattcgcc gcctcgcgct cgcccgcgcg tcgcagctct
     3061 tgctggcgat gggcctgatc ctgctgacgc tcgccgccgg cgagccgcag tggtggcgtg
     3121 tggcgaccag cagcgtcgtg gtcatggtcg atctgtcacc cagcacccgc ggggcgacgt
     3181 tccgccatcg cgatgcgctg atcgcgcggg tccatcagct gcttggcaac cagcc
//
LOCUS       DATIMH010001040         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_537489, whole genome shotgun sequence.
ACCESSION   DATIMH010001040 DATIMH010000000
VERSION     DATIMH010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537489"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="VLI90_18090"
     CDS             complement(<1..1697)
                     /locus_tag="VLI90_18090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV16178.1"
                     /translation="MKSSLFAELRRRNVLRAGAFYIAATWALAQGIAQLGPAVGLPEW
                     ATRWFLVAAIIGFPFWIAFAWLFEFTPEGIKRERNVEPHESITHHTGRKLDFAIIGVL
                     AIAVVLLATDRFVLHFGVKADTAISAQSIAVLPFVDMSQSKDQEYFSDGISEELLNLL
                     AKIQTLHVAARTSSFSFKGKDLPIPEIAKTLHVANVLEGSVRKAGDRIRITAQLIRAA
                     DGYHVWSQTWDRELDDIFKIQDEIAGKVVEELKVTLLGAAPKARTTDPEAYALYLQAI
                     QLGRQGTAEAYQRSDALYRKVLTIDARYAPAWSSLGANLSNEAGQGLLPAKQGYAEAR
                     EAATKALAIDPEYAPAHARLGRIAMYGDNDLAAAAEHFKHAVALDPADLDVMRNSAVL
                     LSNLGRLNEALALDDAVVRRDPVNVSALFNLGLYQRMGGRIDAAITSFRTVLSLSPGS
                     GNAHAQLGTALLLKGQASDALAEIEQETSEVDKMISLPMAYHALGRKAESDAALAALI
                     AKYEKDSPYNIAYVYAYCGEVDNAFAWLDKAVEYGDSGLAEIVSENLLDNIHADPRW"
BASE COUNT          401 a          666 c          659 g          428 t
ORIGIN      
        1 aaccagcggg ggtcggcgtg gatattgtcc agcaggtttt ccgacacgat ctcggcgagg
       61 ccgctgtcgc cgtactcgac tgccttgtcg agccaagcaa atgcgttgtc cacctcgccg
      121 cagtaggcgt aaacgtaggc gatgttgtac ggcgagtcct tttcatactt agcgatcaac
      181 gcggcgagcg cagcgtcaga ttctgctttg cgccccaacg cgtggtacgc cattggcagc
      241 gagatcattt tgtcaacctc gctcgtctcc tgctcgattt ccgcaagcgc gtccgacgct
      301 tggcccttca gcagcagtgc ggtgccgagt tgggcgtgcg cgttgccgct gccagggctc
      361 aggctcagca cggtgcggaa ggacgtgatc gccgcgtcga tccggccccc catgcgctgg
      421 tagagaccca agttgaagag cgccgaaacg ttcaccggat cgcggcgcac gacggcgtca
      481 tcgagcgcca gagcctcatt caggcgtccc agattcgaga gcaggaccgc gctgttgcgc
      541 attacgtcca agtccgccgg atcgagagcc accgcatgct tgaagtgttc cgccgcggcc
      601 gcgagatcgt tatcgccgta cattgcaatc cggccgagcc gcgcgtgggc cggtgcgtac
      661 tccggatcga tcgcgagcgc cttggtcgcc gcttcccggg cttcggcgta gccctgtttg
      721 gcaggtagca ggccttgccc cgcctcgttg ctcaagttgg ccccgaggct gctccacgcc
      781 ggggcgtagc gcgcgtcgat cgtcagcacc ttccggtaca gcgcgtcgga tcgctggtac
      841 gcttcggcgg taccctgccg acccagctga atggcctgca ggtacagcgc gtacgcctcg
      901 ggatcagtcg tgcgcgcctt cggcgcggcg ccgagcagcg tgacctttag ctcttccacg
      961 actttccccg cgatctcatc ctggatcttg aaaatgtcgt caagttcgcg atcccaagtc
     1021 tgcgaccaga cgtggtagcc gtcggccgcg cggatcagct gggcggtgat gcgaatccgg
     1081 tcgcccgcct tgcggacgga tccttccagc acgttggcga catgcagtgt cttggcaatc
     1141 tccggaatcg gcaggtcctt gcccttgaat gaaaacgacg acgtgcgtgc agcgacgtgc
     1201 agtgtctgga tcttcgccaa caggttcagc agttcttcgg agatgccatc ggaaaagtat
     1261 tcctggtcct tcgattgcga catgtcgacg aacggcagca cggcaattga ttgcgcggat
     1321 atcgcagtgt cggccttgac gccgaagtgc agcacgaagc ggtcggtcgc cagcagaaca
     1381 acggcaatgg ccagcacacc gatgatcgca aagtcgagct tgcggccggt gtggtgcgtg
     1441 atcgactcgt gcggctcgac attgcgctcg cgcttgatgc cttccggcgt gaattcgaat
     1501 aaccacgcga acgcgatcca gaacgggaac ccgatgatcg cggcgacgag aaaccagcgt
     1561 gtcgcccatt cgggcaggcc caccgccggt ccgagctgcg caatgccttg cgcgagcgcc
     1621 cacgtcgccg cgatgtaaaa cgcgccagcg cggagcacgt tgcggcgccg cagttcggcg
     1681 aacaggctgc ttttcatgct cggatgcatt ccccctggcc cggagaatgc cgagtgtcgg
     1741 caattacatt ggcgtccgca agaaaaacag cggacagagt gcacttttcg aagcgtgaaa
     1801 cggggtcatg cgggaacggg gcaaagtgcc ttttccaccg aattcacatt tttgaaaccg
     1861 gcaatgttct ctgagcgtat ttggcgttgc cgttcctcaa aaggacgaac ctgcttccca
     1921 acttttagcg ggatatcttt atcaataacg aaaacgcatg aagttcgcgt caatcgttgc
     1981 agcgctactc gccacgacat cgatcaccgt cgcacaggcg gatgaacaca cgcgaccgtt
     2041 gggcaacgat tgcccgtaat cgcgctgcag ttttgccgaa aagttggcgg gctgcgttcg
     2101 gctagccgcc gaatccattg ccgaagatcg tgtcgctggc cgcatagcat gctc
//
LOCUS       DATIMH010001041         1815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5451048, whole genome shotgun sequence.
ACCESSION   DATIMH010001041 DATIMH010000000
VERSION     DATIMH010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5451048"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1366
                     /locus_tag="VLI90_18095"
     CDS             293..1366
                     /locus_tag="VLI90_18095"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSV16179.1"
                     /translation="MSQPALTTTPRPAHPARLFSAIVLLLVGAVMFYVLAVRPRAPKH
                     LKLALLTWTQDPFWEPLIRGAQDCASQANVDLTVVRSKPTVDDQNQHIRELLDAGVDG
                     IAISPNDASAQLAILNDAAARVPIVTFDSDAPSSKRRRFVGIDNYAAGRFSADEIREA
                     LPDGGPILISVGSATMQHGRDRRQGVIDGLLERGFDRNRPSDPIDGELKGPKYTVVAT
                     VTDGADPSKAVVTIAEALRAHPEVKGIVGLFSYSAPAALEAMKQVGRTGQIQVVGFDE
                     SLETQQAIEAGTIHSSILQDSQRAGYQAIEVLANEARGIAPGPAEQSAILNVDINVLT
                     PKNLPLLRESGAIRPATSQPSGT"
     gene            1517..>1815
                     /locus_tag="VLI90_18100"
     CDS             1517..>1815
                     /locus_tag="VLI90_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16180.1"
                     /translation="MVRYLARAVVAGVCLLASTRSSAAIAFNITNQGNASAQMMSGFA
                     QAALIWSAWLMDPITINVRVNALALPAGQLGATGAFYDTFAYADVRQAMLNDRLTL"
BASE COUNT          330 a          643 c          537 g          305 t
ORIGIN      
        1 cacgaccgcc gcgtctgcga aaccgcaagc gactgccgcg ccgatcgcgc gattgacgct
       61 ttcaccgacg actttcctgt tccccaaaaa gttgatgtat caacttctcc gcgcgcgaac
      121 accgctcgtg tttcaatcgc aaatgaagtt gcaaccatcc ccgccccatc tgctatcatg
      181 cccccgtctc cccgccggag ctcaaaacaa attcatcgcg cgatcaacgc gtcacaggcg
      241 cgccccttcg ctgcgatgct acggcggtca tctccactca cggaggccac gcatgtccca
      301 acccgcgctc accaccacgc cgcggccggc gcatccggcg cgactgttca gcgccatcgt
      361 cctgctgctc gtcggcgccg tcatgtttta tgttctcgcc gtgcgcccgc gcgcaccgaa
      421 acatctgaag ctcgccctgc tgacatggac gcaagacccg ttctgggaac cgctcatccg
      481 cggcgcgcag gactgcgcca gccaggccaa cgtcgatctg accgtcgtcc gctccaaacc
      541 aaccgtggac gatcagaacc agcacatccg cgagttgctc gacgccggcg tggacggcat
      601 cgccatcagc cccaacgacg ccagcgctca gctggccatc ctcaacgacg ccgccgccag
      661 ggtgccgatc gtcacgttcg attccgatgc gcccagttcg aagcgccggc gcttcgtcgg
      721 catcgacaac tacgccgccg gacgtttctc cgccgacgag attcgcgaag cgctgcccga
      781 tggtgggccg atcctgatct ccgtcggcag cgcgacgatg cagcacggcc gcgaccgccg
      841 gcagggcgtg atcgacggac tgctggaacg cggcttcgac cgcaaccgcc cgtctgatcc
      901 gatcgatggc gaattgaaag gcccgaagta caccgtcgtc gccaccgtga ccgatggcgc
      961 cgatccgtcg aaggcggtcg tcaccatcgc cgaggcgctg cgcgcgcatc cggaagtaaa
     1021 aggcatcgtc ggcctgttca gctacagcgc tccggccgcg ctggaagcga tgaagcaagt
     1081 cggccgcacc gggcagattc aggtcgtcgg cttcgacgaa agcctcgaaa cgcagcaggc
     1141 gatcgaagcc ggcacgatcc actcgtcgat cctgcaggat tcgcagcgcg ccgggtacca
     1201 agcgatcgaa gtgctcgcca acgaagcccg cggcatcgcg ccggggccgg cggagcagtc
     1261 ggcgatcttg aacgtggaca tcaacgtgct gacgccgaag aacctgccgc tgctgcgcga
     1321 atccggcgct atccggcctg cgaccagtca gcccagcgga acctagaggc tggcaaatcg
     1381 ggccggggac ggcccggctc tcccatatcc gtcgccgctt cgaatcgctt cccgctagcg
     1441 atcgctggac agcgcgtatc gttcatccat tcagagatag cagtccgcaa cgggggttgc
     1501 ttccacggag ttcccgatgg tccgctacct tgcgcgcgcg gtggtggctg gtgtctgctt
     1561 gcttgcatcg acccgatcgt cggcggcgat cgccttcaac atcaccaatc aaggcaacgc
     1621 atcagcgcag atgatgagcg gcttcgcgca ggcggcgttg atctggtcgg cgtggttgat
     1681 ggacccgatc accatcaacg ttcgcgtcaa cgcgctggcg ttgcccgccg ggcagctcgg
     1741 cgcaaccggg gcgttctacg atacgttcgc ttatgccgac gttcgccagg cgatgctgaa
     1801 cgatcggctg acgct
//
LOCUS       DATIMH010001042         1569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1115888, whole genome shotgun sequence.
ACCESSION   DATIMH010001042 DATIMH010000000
VERSION     DATIMH010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115888"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>1569
                     /locus_tag="VLI90_18105"
     CDS             23..>1569
                     /locus_tag="VLI90_18105"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV16181.1"
                     /translation="MANTRIAAWDGKQPAILLTITKQAGANVIETVDRVQQLLPQLMA
                     WMPPDINLSVIQDRTHTIRASVADVQYTMGVTIALVLMVVAMFMRRMMPILAAAVTVP
                     LSIGGTLAGMWFMGYALDNFSLMALTISVGFVVDDAIVMIENIIRHTERGLPPLKAAL
                     IGSRQIGFTVVSISLSLVAVFIPLIFMGGILGRLFHEFAMTLTMAIVISAAVSLTLTP
                     MICGRWMKQRHHVPKRALWARIDRVIERALALNHRIYARTLGWSLRHRGFMLLVMLAT
                     VVVTVQLYRTVPKGFLPIQDTGILMGSTVASPDISFKAMEDRQRAVVDVLLADPAVAT
                     VGSQVGVASGWNSLNRGQLQISLKPLDQRHISSEEVITRLRPKLTNLGGIQTFLYSAQ
                     DLRGGGRSGGSQFQFVLISEDLAALRHWGQLLEDKMKATPGVTDVTSDQDRAGPQANV
                     VIDRTAAARLGVRTTDIDNALNNAFAQRQVSTIYTQRNQYKVVHETDPSLQTSPAMLD
                     RIYVAST"
BASE COUNT          282 a          487 c          494 g          306 t
ORIGIN      
        1 cgtcgctact gtggtgaaca gtgtggctaa cacgcgcatc gcagcgtggg acggcaagca
       61 gccggcgatt ctgctgacga tcaccaaaca ggccggcgcc aatgtgatcg agacggtcga
      121 tcgcgtgcag cagttgctcc cgcaactcat ggcctggatg ccgccggaca tcaatctgtc
      181 ggtcatccag gaccgcaccc acacgatccg cgccagcgtc gccgacgttc agtacacgat
      241 gggcgttacc atcgccctgg tgctgatggt ggtggcgatg ttcatgcgcc ggatgatgcc
      301 gatccttgcc gcggccgtca cggtgccgct gtcgatcggc ggcaccttgg ccggcatgtg
      361 gttcatgggc tacgcgctcg ataatttctc gttgatggca ctcacgatct cggttggatt
      421 cgtggtcgat gatgcgatcg tcatgatcga gaacatcatc cgccacaccg aacgcggact
      481 gccgccattg aaagcggcgc tgatcggctc gcggcagatc ggcttcacgg tggtctcgat
      541 cagcctgtcg ctggtcgctg tgttcatccc cctgatcttc atgggcggca ttctggggcg
      601 cctgttccac gaattcgcca tgacgctgac aatggcgatc gtgatctcgg cggcggtgtc
      661 cctgacgctt acgccgatga tctgcgggcg ctggatgaag cagcgtcacc acgtaccgaa
      721 acgggcgctt tgggcccgca tcgaccgggt gatcgagcgc gcgctggcgc tcaaccaccg
      781 catctatgcg cgcaccctcg gctggtcact gcggcatcgc ggcttcatgc tgctggtgat
      841 gctggccacg gtggtggtga cggtgcagct ctaccgcacc gtgccgaaag gctttctgcc
      901 gatccaggat accgggatcc tgatgggctc gaccgtggca tcgccggaca tttcgttcaa
      961 ggcgatggag gatcgccagc gcgcggttgt ggatgtgctg ctggcggatc cggcggtcgc
     1021 aacggtggga tcgcaggttg gcgtcgccag cggctggaat tcgctcaatc gcggacaact
     1081 ccagatcagc ctgaagccgc tcgatcaacg ccacatttct tcggaggagg tgattacccg
     1141 cttgcggccg aaactgacaa atctaggcgg tatccagacc ttcctgtatt ccgcgcagga
     1201 tttgcgcggc ggtggacgta gcggcgggtc gcagttccaa ttcgtactga tcagcgagga
     1261 cctcgcggcg ctgcgccact ggggccagct tttggaagac aagatgaaag ccacgccggg
     1321 tgtaaccgac gtgacgtccg accaggatcg cgccggcccg caggcgaatg tggtgatcga
     1381 ccgaaccgcc gccgcccggc tgggggtgag gaccaccgat atcgacaacg cgctcaacaa
     1441 cgccttcgcg caacgtcagg tttcgaccat ctacactcag cgcaaccagt acaaggtcgt
     1501 gcatgaaacc gatcccagct tgcagacatc cccggcgatg ctggaccgca tctatgtcgc
     1561 ttccaccag
//
LOCUS       DATIMH010001043         1573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2268543, whole genome shotgun sequence.
ACCESSION   DATIMH010001043 DATIMH010000000
VERSION     DATIMH010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2268543"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="VLI90_18110"
     CDS             <1..1286
                     /locus_tag="VLI90_18110"
                     /inference="COORDINATES: protein motif:HMM:NF028231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSV16182.1"
                     /translation="PHPPRNDWQGSLATLAQLVIQHGVELISIGNGTASRETDKLAAE
                     VIKLVTAKKPEQKLTKIIVSEAGASVYSASAFAAAEFPELDVSLRGAVSIARRLQDPL
                     AELVKIDPKSIGVGQYQHDVNQRALARSLDATVEDCVNAVGVDINTASAALLTRVSGL
                     NKVLAKNIVEYRDANGPFPNRTTIREVPRLGDKTFEQAAGFLRINNGDNPLDRSSVHP
                     EAYPVVERILTRINKGIGEVMGQPTLLKGLSAADFTDEKFGVPTVRDILTELEKPGRD
                     PRPEFKTATFQEGVESLSDLQPGMILEGVVTNVAAFGAFVDIGVHQDGLVHVSALAHT
                     FVKDPHDVVKPGQIVKVRVMEVDVKRQRISLTMRLDEPSAPLSRRDRPRVDTQTAAPS
                     GAHGRRPQPPQRAPEREPHTNGAFAMALARAKLKK"
     gene            complement(1327..>1573)
                     /locus_tag="VLI90_18115"
     CDS             complement(1327..>1573)
                     /locus_tag="VLI90_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16183.1"
                     /translation="LLTGALLSRFTDSSLDMSPTFIGKGTTLLQLLYIILIIIVTSRH
                     LERQLLQPLLYLMVFLTVTSGLHYLYRGVTHLNTREA"
BASE COUNT          350 a          463 c          481 g          279 t
ORIGIN      
        1 atccgcaccc gccgcgcaat gattggcagg gctcgctcgc gacgctggct cagcttgtaa
       61 tccagcatgg cgtcgaactg atctcgatcg gcaatggcac cgccagccgc gagacggaca
      121 agctcgccgc cgaggtgatc aagctggtca ccgccaagaa accggaacag aagttgacca
      181 agatcatcgt cagcgaggcc ggtgcatcgg tctattcagc gtcggccttc gccgcggcgg
      241 aattccccga gctggacgtg agcctgcgcg gcgccgtctc catcgcccgt cgcttgcaag
      301 atccgctggc cgaattggtc aagatcgatc ctaaatcgat cggcgtgggc caatatcagc
      361 atgacgtaaa ccagcgcgcc ttggcgcgct cgctcgatgc gactgtcgaa gactgcgtga
      421 acgccgtggg cgtggacatc aatacggcgt ccgccgcgct actcacacgg gtctccggtc
      481 tcaacaaggt gctggccaag aatatcgtcg agtaccgcga tgccaatggg ccgttcccga
      541 atcggaccac gatacgcgag gtgccgcgat tgggagacaa gacgttcgag caggccgcag
      601 gctttctacg gatcaataac ggcgataatc cgctggatcg ttcttcagtc cacccggagg
      661 cttatccggt agtcgagcgc attctgacgc gcatcaacaa aggcatcggc gaggtaatgg
      721 gccagccgac cctgctgaaa ggactgtccg ccgccgactt caccgatgag aaattcggtg
      781 taccgacggt acgcgacatt ttaactgagc tggaaaaacc aggccgcgat ccgcgtccag
      841 aattcaaaac ggccaccttt caagaaggcg tcgagtcgct gtccgacctg cagccgggaa
      901 tgattctcga gggcgtggtg acgaacgtcg cggcctttgg cgcgttcgtc gatattggcg
      961 tgcaccagga cggcctcgtg cacgtatccg ccttggcgca tacattcgtg aaagaccccc
     1021 acgatgtggt gaagccgggc cagatcgtca aggtgagggt catggaggtc gacgtgaagc
     1081 gccagcgcat ttccctgacc atgcgcctcg acgagccatc ggcgccatta tcgcgtaggg
     1141 atcgtcctcg ggtggacaca cagaccgctg ccccgagcgg cgcgcatggc cggcgtccgc
     1201 aaccgcctca gcgcgctccg gaacgcgagc cgcacaccaa cggcgcgttt gccatggcgt
     1261 tggcgcgggc gaagttgaag aagtaacagc gtggggacgg ccgtctgccg atcgtgatct
     1321 cgtccgctac gcttcacgag tattgagatg ggtgacaccg cggtacaaat aatgaagacc
     1381 tgaagtcact gtgagaaaga ccatgagata cagaagcggc tgcaggagtt ggcgttcgag
     1441 atggcgtgag gtcacgataa taatcagaat aatgtataac aactgcaaca aggtcgtgcc
     1501 tttaccgatg aaagtcggcg acatatcgag cgatgagtcc gtaaaacggc tgagcaacgc
     1561 cccggtcagc aaa
//
LOCUS       DATIMH010001044         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3137081, whole genome shotgun sequence.
ACCESSION   DATIMH010001044 DATIMH010000000
VERSION     DATIMH010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3137081"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VLI90_18120"
     CDS             <1..884
                     /locus_tag="VLI90_18120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSV16184.1"
                     /translation="NLKAQQDTLDLTRSRFKAGLTSDLDVARAQALVATTAAQVPALE
                     IQIKQTIHRLGILLGEEPMALAAELGKTAPIPPSPSEVPVGLPSELLRRRPDVRQAER
                     QLEASTARIGVAVAQLFPRFSLTGSLGQESGRFGLLARGDSTFWSFGPSVTWRVFDTN
                     QLINQVRVANAQQQASLINYKRTVLQSFSDVEDALVAYSQDQNRTRSLADSVAANQRA
                     VDLSNQLYARGLGDFLNVLDAERQLYAAENDLTISEAAVGSDLVQLFKALGGGWDETN
                     EKEFEKHEDPKQKVAME"
     gene            988..1599
                     /locus_tag="VLI90_18125"
     CDS             988..1599
                     /locus_tag="VLI90_18125"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSV16185.1"
                     /translation="MLKLFPTTLEGHGVRLEPLTVQHTEGLAAAAADGRLWELWFTSV
                     PAPHEVGPYIETALAGQSAGHMLPWAVRELASGAMVGSTRYHDIVPAVERVEIGYTWY
                     AARCQRSHVNTACKLLLLQHAFEYAGCAVVGFRTDNFNFRSQRAIEALGAKKDGVIRH
                     HQARRDGTVRDSVMYSILAAEWPDVRRHLQLRLARHATDEVRT"
     gene            complement(1938..2762)
                     /gene="kdsA"
                     /locus_tag="VLI90_18130"
     CDS             complement(1938..2762)
                     /gene="kdsA"
                     /locus_tag="VLI90_18130"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HSV16186.1"
                     /translation="MSTNLNLTRSPLFVIAGPCVIESAELCLTIGRHVKDVCDKLGLV
                     YVFKASFDKANRSSSASFRGPGIAEGLSILAGIKRELNVPVLTDIHESDQAAEAAEVV
                     DILQVPAFLARQTDLLLACGRTGKTVNVKKGQFMSPQEMSLVVDKIKSTGNTQITLTE
                     RGTFFGYNRLVNDFTALAIMKSLHVPIIFDITHSTQQPAGLGNQSGGNPQFSPLLARA
                     AIAAGVDGLFVECHPDPRNAKSDAATMMNLNEVEPLLTQCKQLAELRGTWNVDEQI"
BASE COUNT          596 a          936 c         1105 g          595 t
ORIGIN      
        1 acaatctcaa agctcagcag gatacgctgg acctgacgcg gtcgcgcttc aaggcgggat
       61 tgaccagcga cctggacgtc gcccgcgcgc aggcgctcgt agccaccacg gcagcacagg
      121 tgccggcgct ggaaatccag atcaagcaga cgatccaccg cctgggcatt ttgctcggtg
      181 aggaaccgat ggcgctggcg gcggagctgg gcaagacggc gccgatcccg ccgtcaccgt
      241 cggaagtacc ggtcggtttg ccgtcggaac tgcttcggcg tcggccggac gtgcgccagg
      301 cggagcggca actggaagca tccaccgcgc gcatcggcgt ggcggtcgcg caactgttcc
      361 cgcggttctc gttgacgggg tcgctgggcc aggagagcgg tcgattcggc ctgctcgccc
      421 gcggcgacag cacgttctgg tcgttcggcc cgtccgtcac gtggcgcgtg ttcgacacga
      481 accagctgat caaccaggtg cgcgtcgcca acgcccagca gcaggcgtcg ctgatcaact
      541 acaagcgcac ggtgctgcag agcttttcgg atgtggaaga tgcgttggtg gcgtattcgc
      601 aggatcagaa tcgaacgcgg tcgctggcgg attccgtggc ggccaaccag cgcgcggtcg
      661 atctttcgaa ccagctatac gcccgcgggc tgggtgactt cctgaacgtg ctggatgccg
      721 agcggcagct gtacgcggcg gagaacgatt tgacgatcag cgaagcggct gttgggtcag
      781 acctggtgca gctcttcaaa gccctcggcg gcgggtggga tgagaccaac gagaaggaat
      841 tcgagaagca cgaggacccg aagcagaagg tggcgatgga gtaacttctc cattgcaggc
      901 cacgtagcgg tcggcctgca gggacacggg tctttcccgc gaacgaaaaa catgtgcccc
      961 tgcgctgcgg ggcgaccgct acgcggcatg ctcaagctat tcccgacaac gctcgaaggc
     1021 cacggcgtgc gtcttgaacc gctgacggtt caacacaccg aaggtctcgc cgccgcggcg
     1081 gccgacgggc ggctttggga gttgtggttc acatccgtgc ccgcgccaca tgaggttggg
     1141 ccgtacatcg agacggccct cgcggggcaa tccgcgggtc acatgctccc ctgggccgtg
     1201 cgcgaattgg ccagcggcgc aatggtcggc agcacccgct atcacgacat cgttccggcg
     1261 gtcgagcgcg tcgagatcgg ctacacctgg tacgccgccc gctgccagcg cagccacgtc
     1321 aacaccgcct gcaaactgct gctgctgcaa catgccttcg aatacgctgg ctgcgccgtc
     1381 gtcggcttcc gcactgacaa cttcaacttt cgctcacaac gcgccattga agcgctgggc
     1441 gcgaaaaagg acggcgtcat ccgccatcat caggcgcgcc gcgatggcac cgtgcgagac
     1501 tcggtgatgt acagcatcct cgccgccgag tggccggacg tgcggcggca tctacaactg
     1561 cgactggcgc gtcatgcgac cgatgaagtg cgcacgtgac gatgccgccg tccgcagagg
     1621 cttgtcagcg cgggtccgaa ccatgtgatg ctcggcggtt ccggtaccga ttcagcgcgt
     1681 tgcccgtaga ccagtggacg tgctggtcaa atccgccgct actgacgtaa tcatcgcctg
     1741 aatctaaatc gtcccgcgcg acgccggtga ctgaggtatt cccaaggcga gggcaatggt
     1801 gaggtatttt gccgttgagt gggcagcggg cggccgtacc acccgaccgc tgatcggcgc
     1861 gagggccgcg ccgagcggat tcgttcggac tcagcgccgc cggtgcgtgg ggagcttgga
     1921 tcggcgttca tcggcgctca gatctgttca tcaacgttcc acgtcccccg cagctccgcg
     1981 agttgcttgc actgcgtgag cagcggctcg acctcgttta agttcatcat cgtcgcggca
     2041 tcgctcttgg cgttgcgcgg atcggggtgg cattcgacga acaatccatc aacgcccgcg
     2101 gcgatggcgg ctcgggcgag caggggggag aactgcgggt tgccgccgct ttggttgccc
     2161 aggccggcgg gttgttgggt gctgtgggtg atgtcgaaga tgatggggac gtgcagggac
     2221 ttcatgatgg ccagggcggt gaagtcgttg acgaggcggt tatagccgaa gaacgtgccg
     2281 cgttcggtga gggtgatctg ggtgttgccg gtcgatttga tcttgtcaac gacgagggac
     2341 atttcctgcg ggctcatgaa ctggcccttc ttgacgttaa cggttttgcc ggtgcggccg
     2401 caggcgagga gcagatcggt ctgccgcgcg aggaacgcgg ggacctgcag gatgtcaacg
     2461 acttcggcgg cctcggcggc ttggtcgctt tcgtgaatgt cggtcaagac tggaacattc
     2521 agctcgcgtt tgatgccggc gaggatcgag aggccttcgg cgatgccggg cccgcggaag
     2581 ctggcgctgc tgctgcggtt ggctttgtcg aagctggcct tgaagacgta gacgaggccg
     2641 agcttatcgc agacgtcttt gacgtgccgg ccgatcgtga ggcagagttc ggcggattcg
     2701 atcacgcaag ggccggcgat gacgaagagg ggcgagcggg tgaggtttaa gttggtgctc
     2761 atggagaagg tgttttcggt ggcggacggc gggttgtaaa gcctcggcga agaagtcgcg
     2821 gcagggggcg cctccgattg ccagtgcttg cgaaccgaac aaatctttcg cgacaggcct
     2881 tgcaaaggtt ggcagcaacg atatattccc gcctcccttg gaccggacgt ctttaggaag
     2941 atgttcgtga acctaagggc gtcggtggcg taatgttaga gctggccgac gcggtgttga
     3001 ccggacgcga aaaaatcaag cgacatggct tgacgcgacg aaaacgctgg atatactctg
     3061 cgacccgccc tcaagtgagg gttttgataa ggaagcgaga cgagtcaccc gcagaaggat
     3121 aagcagattg gtctatccaa cagctttgat caccccgaag tagctcgcag ttgaccacac
     3181 acaatcaggt cactggcgag aaccggtgac gcgactcttg aaggacggca gt
//
LOCUS       DATIMH010001045         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_828735, whole genome shotgun sequence.
ACCESSION   DATIMH010001045 DATIMH010000000
VERSION     DATIMH010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828735"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VLI90_18135"
     CDS             complement(<1..229)
                     /locus_tag="VLI90_18135"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HSV16187.1"
                     /translation="MLRFLTDQNFNGDILRGMLLRQPGLDVVRIQDEPVIDGADDPDI
                     LEWADRNNRIVLTHDKNTLPTSHMRGWELAGE"
     gene            complement(241..573)
                     /locus_tag="VLI90_18140"
     CDS             complement(241..573)
                     /locus_tag="VLI90_18140"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HSV16188.1"
                     /translation="MPSIAVQAEAPPIRMEPDGSYRVGTSRVLLDIVIGTFQLGATPE
                     TIVQQYPSVALGDVYAVIAHYLRHKPEVDAYLAAREREAEEIRARIEKSQPDLADIRA
                     RLLAHRHG"
     gene            703..>1695
                     /locus_tag="VLI90_18145"
     CDS             703..>1695
                     /locus_tag="VLI90_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSV16189.1"
                     /translation="MPAAAPALGQQAADFLQKFGLVKGALLKELHKVIIGQDEVIELL
                     LAGLFARGHCLLVGVPGLAKTLMISTLARVLKLNFNRVQFTPDLMPADITGTDILEED
                     QATGKRAFRFVKGPVFANIVLADEINRTPPKTQAALLQAMQEYHVTAGGRTYPLELPF
                     FVLATQNPVEQEGTYPLPEAQLDRFMFMVNIGYPTKAEERAIVRATTMELEATPEAVL
                     SASDITHVQKVLRKLPVSDHVVDYAVSLTRATRPKEPNTPAFINDWLTWGAGPRAAQY
                     LVLGAKARAILDGRLNVGCDDVRKVAKPVLRHRLFTNFNADAEGVGPDQVIEKLL"
BASE COUNT          333 a          498 c          544 g          320 t
ORIGIN      
        1 attcgcctgc cagttcccat ccgcgcatat gcgaagtcgg gagagtattt ttatcgtgcg
       61 tcaagacaat gcggttattt cggtccgccc attcaagaat gtccgggtcg tccgctccgt
      121 cgattactgg ttcatcctgt atccgcacca catccaggcc gggctgtcga agcagcattc
      181 cgcgaagaat gtcgccgttg aaattctggt ccgtcaagaa ccgcagcatc gcgagatctc
      241 ttatccgtgg cgatgggcca gcaacctcgc gcgaatatcg gccagatcag gctgactttt
      301 ttcaatccgc gcgcgtatct cttccgcctc gcgctcgcga gcagccaagt aggcatcaac
      361 ctccggctta tgacgcaggt aatgtgcgat caccgcatac acatcgccca gcgcgacgga
      421 cggatattgt tgcacaattg tctctggcgt cgcgccaagt tggaacgtcc cgatcacgat
      481 gtccagcagc acgcgcgagg tgccaacacg gtagctgccg tcaggttcca tgcggattgg
      541 aggcgcttca gcttgcactg cgatcgaagg catggtggag attatatcca ttcgacaata
      601 cactcgccaa ataacgcgaa gcgtcgtggt taggtgcgcg aaggaaccgc tttgtctcaa
      661 gtccctaaaa cttcactacc gccgacacca cccccgccgc ccatgcccgc tgcggcgccg
      721 gcactcgggc agcaagccgc tgacttcctg caaaaattcg gcctcgtcaa aggcgcgctg
      781 ctcaaggaac tgcacaaggt catcatcggc caggacgaag tgatcgagct gctgctggcg
      841 gggctgttcg cgcgcgggca ctgcctgctc gtcggcgtgc cggggttggc gaagacgctg
      901 atgatctcga cgctggcgcg cgtgctgaag ttgaacttca atcgtgttca gttcacgccg
      961 gatttgatgc cggcggatat cacgggaacc gacatcctcg aggaagatca ggcgacgggg
     1021 aagcgggcgt ttcggttcgt caaaggtccc gtcttcgcga acatcgtttt ggcggacgag
     1081 atcaaccgca cgccgcccaa gacgcaggcg gctctgcttc aggcgatgca ggaatatcac
     1141 gtgacggcgg gggggcggac gtatccgctg gagctgccgt tcttcgtgct ggcgacgcaa
     1201 aacccggtgg agcaggaggg gacgtatccg ctgccggagg cgcagctcga ccgcttcatg
     1261 tttatggtga atattggata tccaaccaaa gccgaggagc gcgcgatcgt gcgggcgacg
     1321 acgatggagc tggaggccac gccggaggcg gtgctgtcgg ccagcgacat cacgcacgtg
     1381 cagaaggtgc tgcgaaagct gccggtgagc gatcacgtcg tcgattacgc ggtgagcctg
     1441 acgcgcgcga cccggccgaa ggaaccgaac acgccggcgt tcatcaatga ctggctgacg
     1501 tggggcgccg gcccgcgggc ggcgcagtac ctggtgctgg gggcgaaagc gcgggcgatc
     1561 ttggacgggc ggttgaacgt gggttgtgat gatgtgcgga aggtcgccaa gccggtgctg
     1621 cggcaccggt tgttcaccaa cttcaacgcc gacgccgagg gcgtggggcc ggaccaggtc
     1681 atcgaaaagc tgctg
//
LOCUS       DATIMH010001046         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5164137, whole genome shotgun sequence.
ACCESSION   DATIMH010001046 DATIMH010000000
VERSION     DATIMH010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5164137"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VLI90_18150"
     CDS             <1..504
                     /locus_tag="VLI90_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16190.1"
                     /translation="VFAALLLVPAFAKADGNIARDAGPFEVTVNGTGSNDSKFHTGSG
                     AIGGSFGWFATPNLELGLRDNASYSDFGSGSSWSNNVRVALDVNLNFDRIQPFIGGNV
                     GYLSGPDTHTAEAAPEGGVKIFLTNQAFLYGMVEYNFFWHTTNNGVAGNASHGQFLYG
                     VGLGLRF"
     gene            complement(595..2376)
                     /gene="recJ"
                     /locus_tag="VLI90_18155"
     CDS             complement(595..2376)
                     /gene="recJ"
                     /locus_tag="VLI90_18155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HSV16191.1"
                     /translation="MQRPKRWTIAETNPLAEELAARLKISTLVSQILLNRGMIEIQDC
                     QDFLKPSLKCLHDPALLANLPAASQRIAKSIRDREKIVVYGDYDVDGITATSILWHAI
                     RTLGGIVEYYIPHRLEEGYGLNAEAVAEICDGGAKLIVTVDCGVTALGPAKVARDRGV
                     DLIITDHHEWHEQAAPLTTADDHEPLLPQCYAIVHPRLPGANGQPYPNPSLCGAGVAF
                     KLAWGIGTAMSGANRVNEEFRAFLLEATGLAALGTIADVVPLIGENRVLAAFGLSGLR
                     QSRLTGIKALIESASLAGQALDAYHVGFLLAPRINASGRMGHAKLAVELLTEASPERA
                     TEIATFLEQQNRERQGIEKGILQQAIEQATALGYDNEDCRAIVLGAEGWHAGVIGIVA
                     SRIVDRFHRPTIMIALNNGHGQGSGRSVAGFHLARALEACSDHLDGYGGHEMAAGLKI
                     DSTRFEGFRSAFCEYATRTLQPEQLVPELKVEAVAEIRQVSQALVTDLQRLGPFGHGN
                     RRPVLCCRDVIIAAPPRRVGKSGDHLQLFVRQGMATMKCIAFNRGAWLDQLTPGTRVD
                     LAVEPTLNEFNGRTSVEMEIKDLHLHG"
     gene            complement(2565..3035)
                     /locus_tag="VLI90_18160"
     CDS             complement(2565..3035)
                     /locus_tag="VLI90_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16192.1"
                     /translation="MPPDSAHLGQQIVWLVVLAIPISSIVWTVTHEEVFREPREYCKR
                     KSEMCASLLKRKFFYLFTCEYCFSHYVTALALVFTRFKLLLDDWRGYVVALFALVWVA
                     NAYMSLFGRLRVEIRSERAAADVKAREVKVKEKEIEHLETRLAGTRDDRNDAAA"
BASE COUNT          654 a         1187 c          945 g          650 t
ORIGIN      
        1 gtcttcgcag ctctgctgct ggtccccgcc ttcgcaaagg ccgacggcaa catcgcccgc
       61 gacgccgggc cgttcgaagt caccgtcaac ggcactggat cgaacgacag caagttccac
      121 acaggcagcg gggccatcgg cggcagcttc ggttggttcg ccacgcccaa tctcgagctc
      181 ggcctccgcg acaacgccag ctacagtgac ttcggcagcg gcagcagctg gagcaacaac
      241 gtccgggtgg ccctggatgt gaacctgaac ttcgaccgca tccagccatt catcggtggc
      301 aatgtcggtt atctctccgg cccagacacc cataccgctg aagctgcccc cgaaggcggt
      361 gtcaagatct tcttaaccaa ccaggccttc ctctacggca tggtcgaata caacttcttc
      421 tggcacacaa ccaacaacgg tgtcgccggc aacgccagcc acgggcagtt cctgtacggc
      481 gtggggttgg ggttgcgctt ctaacgaagc gaatggataa tggtcaatgg aaaatggcca
      541 atggaaagag gcgcgcgata tcgcctctga ccatttgcca ttttccgttg accattatcc
      601 gtggagatgg agatccttga tctccatctc cacgctcgtc cgcccgttga attcgttcag
      661 cgtcggctcg accgccagat ccacgcgggt ccccggtgtc agttgatcga gccacgcccc
      721 gcggttgaac gcgatgcact tcatcgtcgc catgccctgc cgcacgaaca gctgcaggtg
      781 atccccggac ttacccacgc gccgcggcgg cgccgcgatg atcacatcgc gacaacacaa
      841 caccggccgg cgattgccgt gaccgaacgg gccaagccgc tgcaggtccg tcaccagcgc
      901 ctggctcacc tgccggatct ccgccaccgc ttccaccttc aactccggca ccagttgttc
      961 cggctgcagc gtccgcgtcg catattcaca aaacgccgaa cgaaacccct cgaaccgcgt
     1021 gctgtcgatc ttcaaccccg ccgccatctc atggccgcca taaccatcga gatgatcgct
     1081 gcacgcctcg agcgcgcggg ccagatgaaa ccccgcgacg cttcgcccgc tgccctgccc
     1141 gtggccgttg ttcagcgcga tcatgatcgt cggccgatga aaccgatcga cgatgcgcga
     1201 cgcgacgatg ccgatcaccc ccgcgtgcca gccctcagcg cccagaacaa tcgcccggca
     1261 atcttcgttg tcatatccca gcgccgtcgc ctgctcgatc gcctgctgca gaatcccctt
     1321 ctcgatcccc tgccgctcac ggttctgctg ctccaggaac gttgcgatct ccgtcgcccg
     1381 ctcgggtgac gcttcggtca acaactccac cgccagcttc gcatgcccca tccgcccgct
     1441 ggcgttgatg cgcggcgcga gcaggaagcc aacgtgatat gcatccagcg cctgccccgc
     1501 gagcgacgcc gattcgatca gcgctttgat gccggtcaat cggctctgcc gcaggccgct
     1561 caaaccgaac gccgctagca cgcggttctc gccaatcagc ggcacgacgt ccgcgatcgt
     1621 ccccagcgcc gctaatcccg ttgcttctaa tagaaatgcg cgaaactctt cgttgacgcg
     1681 gttcgcgccg ctcatcgccg tgccgatgcc ccacgccagc ttgaacgcca ccccggcccc
     1741 gcacagagag ggattcggat acggctgccc gttcgcgcct ggtagccgcg gatgcacaat
     1801 tgcgtaacac tgcggcagca gcggttcatg gtcgtccgcc gtcgtcagcg gcgccgcctg
     1861 ctcgtgccat tcgtgatggt cggtgatgat cagatcgacg ccgcgatcgc gcgcgacctt
     1921 ggccggcccc agcgcggtca cgccgcaatc gaccgtgacg atcagcttcg ccccgccgtc
     1981 gcaaatctcc gcgaccgcct cggcgttcag cccgtatccc tcttccagcc gatgcggaat
     2041 gtagtactcg acgatgccgc ccagcgtgcg gatcgcgtgc cacaggatgc tggtggccgt
     2101 gatgccatcg acgtcgtaat cgccgtagac gacgatcttc tcgcgatcgc gaatggactt
     2161 ggcgatccgc tgcgacgccg ccggcaggtt cgccagcagc gccggatcat gaaggcactt
     2221 aaggcttggc ttgagaaagt cctggcagtc ctgaatctcg atcattccgc ggttgagcaa
     2281 gatttgactg actaaggtgg agatcttcaa ccgcgcagcc agctcttcag ccaacgggtt
     2341 ggtctcagcg atcgtccagc gcttcggtcg ttgcatccgt gtctaagcct cgccccgcgg
     2401 aatccgttca aaccaggcaa ggattttacg gctgcgaacc caaacattct acgagattga
     2461 aactgaacgc agagacgctg aggccgcaga gaaatttccg agaagtcata cctgccgcat
     2521 ttctccgcgg cctctgcgtc tctgcgttca aatttgtctt ccggttacgc cgccgcgtcg
     2581 ttgcggtcat cgcgcgtgcc cgccaaccgc gtttccaggt gctcgatctc cttctccttc
     2641 accttcacct ctcgcgcttt cacatccgcc gcggcgcgtt cgctgcggat ttccacgcgc
     2701 agccggccga acaggctcat gtacgcgttt gccacccaca ccagcgcgaa cagcgccacc
     2761 acatacccgc gccaatcgtc cagcagcagc ttgaaccgcg tgaagaccag cgccagcgcc
     2821 gtcacgtaat ggctgaagca atactcacac gtgaagagat agaagaactt ccgcttcagc
     2881 agcgacgcgc acatctcgct cttgcgcttg caatattcgc gcggctcgcg aaagacctcc
     2941 tcgtgcgtga ctgtccatac gatgcttgag atcggaatcg cgagcacgac gagccagacg
     3001 atctgctgac caagatgagc ggaatcgggc ggcatctcgc ccatctatat cccccgccgc
     3061 gcccccgcgg gaacgggcga agaagcggac tgttatcggc tgttcgcctg caggtcctgc
     3121 cccctcgccc ggtatgccgg gagagggctg gggtgagggt gatttgcgaa tgacgaaatt
     3181 cgaaatccga atccccgaat gaatgacgaa atccgaatgg cgaatgacga gccgcgcgcg
     3241 atggcatgcg cttcgtcatt cgtcattcag atttcattcg ggattcggat ttcgtcattc
     3301 gggtttcctc tagaaaatca ccctcacccc agccctcttc cggggcaccg ggctgacgac
     3361 ccgacacttt tgcaggaatg gctgggaacc gaccactttt gtaaaagtgt ccgaccgtca
     3421 ggagaaccgg gcgagc
//
LOCUS       DATIMH010001047         3780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_830692, whole genome shotgun sequence.
ACCESSION   DATIMH010001047 DATIMH010000000
VERSION     DATIMH010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830692"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VLI90_18165"
     CDS             <1..253
                     /locus_tag="VLI90_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16193.1"
                     /translation="FRFHHGNNPHDEPVYNIDVAWPDDARIIRAHDFGLPKDEEIFRY
                     YAQRDPQRVFYIFDRADDSLTELGTGQSLAQRYHDDASK"
     gene            309..578
                     /locus_tag="VLI90_18170"
     CDS             309..578
                     /locus_tag="VLI90_18170"
                     /inference="COORDINATES: protein motif:HMM:NF044561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSV16194.1"
                     /translation="MLQRYHTIIKPETNGWFVGWVEEIPGTITHGKSLDECRGKLRDA
                     LELMLETHRHEARLGLSARCIQECIEIEVGDGAEAAERCGMQYPA"
     gene            complement(698..2017)
                     /locus_tag="VLI90_18175"
     CDS             complement(698..2017)
                     /locus_tag="VLI90_18175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV16195.1"
                     /translation="MTALPSAADVLYLKDGTKIEGDIKHSDNGWLVYRNGKSTLVFPE
                     QVKSIELSASPDAAPKAAAERLASLRRSVETLNDPRDVIARYQHFIEQVKDPAAVAEA
                     KRDMASWQDRADQNMVKVGSKWILPGERAKLSDEAMGLAEQARQLMKQGRAKEAEPLL
                     VEATADDPQNVSALYLTALLRYQQDQLPAARKALDAVAQLVPNHAPTLNNLGVVQWRQ
                     RQYVAALISFDGAMLAAPVNKDILDNVASALQALPAEFRNSNVTQRVARRFQEQDRQL
                     AEQMAQAGWHRVGTGWVTDKDYDELKKQEKETQDKLDALAGEFDKAKQHLADLDTRIA
                     DTEEQINRIDATSYVRDPASGTFLRIPYPDVYYRLQKDDARLKQERQTEVAHMDDMRE
                     TAKGLKQRQVIGKTVEVQRMIGPEGTPIREIETLSTRPATHPAETQE"
     gene            complement(2149..3345)
                     /locus_tag="VLI90_18180"
     CDS             complement(2149..3345)
                     /locus_tag="VLI90_18180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV16196.1"
                     /translation="MHYFRLLRQRGIEAWLVVNERTRGELETLLASDIDRVRFVPNTR
                     FHQRLDRVALLFPPVIKHFLFRFIGRLVSARTARRIVREMVAAHRIDIVHQPTPVSPK
                     EISLMYKVGAPVIIGPMNGGITYPPAFRQIQKRWVRAFVGVGRLISHAFNWLMPGKLR
                     AQTLLVANDRTRRALPTGVRGRVEQLVENGVDLNLWNANERSRNLQIEPVRFVFSGRL
                     EEWKGVQYLLQAFGQVVQQMPATLEIMGSGVMRQRLEAQAAAAGLSGSVTFRGWVSQP
                     ECVAILSAADVFVLPSLYECGGAVVLEAMAVGLPVIATNWGGPADYLDPSCGVLVDPK
                     SPEAFVQQLAEAMLRLGRDASLRHSMGAAGRAKAVAQYDWQRKIDQILRIYSETKQQH
                     KTTTRA"
     gene            complement(3482..>3780)
                     /locus_tag="VLI90_18185"
     CDS             complement(3482..>3780)
                     /locus_tag="VLI90_18185"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HSV16197.1"
                     /translation="DFETLRANVGDTENLINIPLQIRAVEVSLLFTEPMDGGAIRVSL
                     RSKGGVDVARFAEQFGGGGHARAAGLKVNGPLQAVHDQVVEAMVAKLETVKVER"
BASE COUNT          687 a         1331 c         1040 g          722 t
ORIGIN      
        1 gtttcgcttt catcacggca acaacccgca cgatgaaccg gtttacaaca tcgatgtcgc
       61 gtggcccgac gatgcgcgca tcatccgggc gcacgatttc ggtctaccaa aggacgagga
      121 gattttccgc tactacgccc agcgcgaccc gcagcgcgtg ttctatatct tcgaccgcgc
      181 cgacgactcg ttgacggagc tgggaaccgg ccagtcgctg gcccagcgct atcacgacga
      241 cgcctcgaag tgagatcaat tgaaagtttg ccttgactct cgccacgcgc ttgcaccaga
      301 atgatgtcat gttgcagcga taccacacca tcatcaaacc ggaaacgaac ggatggttcg
      361 tgggatgggt tgaagagatc cccggaacca tcacgcatgg aaagtcgctc gacgagtgcc
      421 gcggcaagct gcgcgatgcg ctcgagctga tgctggagac gcaccggcac gaagcccgcc
      481 tgggcctgag cgcgcggtgc atccaggaat gcatcgagat cgaagtcggc gacggcgccg
      541 aagccgccga gcgatgcggg atgcaatatc ccgcctgacc cggccgcagc gcgcgcgttc
      601 gtttccgctg ttccaggtgc acccagcgcg catgcgcaca catcaccgcc gggcgatcga
      661 tctgtctgcg ccgctatatc accggaattg tgaagcgtta ttcttgcgtc tccgccggat
      721 gtgtcgccgg ccgggttgac agcgtttcga tctcgcggat cggcgtgccc tcgggtccga
      781 tcatgcgctg tacttccacc gtcttgccaa tcacctgccg ctgcttcagg cccttggccg
      841 tctcgcgcat gtcgtccatg tgcgccactt ccgtttgccg ttcctgcttc agtcgcgcat
      901 cgtccttctg tagccgatag taaacatcgg gatagggaat tcgcaggaaa gtgcccgaag
      961 ccgggtcgcg cacgtagctc gtcgcgtcga tgcggttgat ctgctcctcg gtgtcggcga
     1021 tgcgcgtgtc caaatccgcc aggtgctgct tggccttgtc gaactccccc gccagcgcgt
     1081 ccagcttgtc ctgcgtttcc ttctcctgct tcttcagctc atcgtaatct ttatcagtga
     1141 cccaaccggt gcccacgcgg tgccaacccg cctgcgccat ctgctcggcc aactggcggt
     1201 cctgttcctg aaatcgccgg gcaacgcgtt gcgtcacgtt gctgtttctg aactccgccg
     1261 gcagcgcctg cagagcgctg gcaacgttgt cgagaatgtc cttgttgacc ggcgccgcca
     1321 gcatcgcgcc gtcaaagctg atcagcgccg ccacgtattg ccgctgccgc cactgcacca
     1381 cgcccaggtt gttcaacgtc ggcgcatgat tcggaaccag ttgtgccacc gcgtcgagcg
     1441 cttttcgcgc cgccggcagc tggtcctgtt gatagcgcag cagcgcggtc agatacagcg
     1501 ccgatacgtt ctgtggatca tccgccgtcg cctcgaccag caacggctcc gcttccttcg
     1561 cccgcccctg cttcatgagc tgccgggcct gctcggccaa tcccatcgcc tcatccgaca
     1621 acttcgcgcg ctcgcccggc agaatccact tgctgccgac cttcaccatg ttctggtcag
     1681 cccgatcctg ccagctggcc atgtcgcgct tcgcttccgc caccgctgcc gggtccttta
     1741 cctgctcaat gaaatgctgg taccgcgcaa tcacgtcgcg cggatcgttc agcgtctcca
     1801 ccgatcgccg cagcgatgcc aatcgttccg ccgccgcctt cggcgcggca tccggtgaag
     1861 ccgacaactc aatcgatttg acctgctccg gaaaaacgag tgtcgatttg ccgttgcgat
     1921 agacaagcca accgttgtcg ctgtgcttga tgtccccttc gatcttcgtg ccgtccttca
     1981 gataaagcac gtccgccgcc gatggcaatg ccgtcaccga caacgcaccc agtgcgccta
     2041 ctgcaaacgc atgccatggc aatcgtcgtc tcatcgtcca agccgccgaa acccaatgat
     2101 agaccagtgt aaccggtgaa agttgggcgc ggccagcaga aatgattctt acgcgcgggt
     2161 tgttgttttg tgctgctgct tggtttcgga ataaatccgc aggatctggt caatcttccg
     2221 ctgccaatcg tactgcgcga ccgccttggc tcggcccgcg gcacccatgg agtgccgcaa
     2281 cgatgcgtcg cgccccagcc gcagcatcgc ctcggccagc tgctgcacga acgcttccgg
     2341 ggacttcgga tcgaccagca caccgcacga aggatccaaa taatccgccg gcccgcccca
     2401 gttcgtcgca atcaccggca ggcccaccgc catcgcttcc aggacgacgg ccccgccgca
     2461 ttcgtaaaga ctcggcagca cgaacacatc cgccgctgac agaattgcga cacactccgg
     2521 ctgcgatacc catccgcgaa aagtaacgct accgctcaat cccgccgccg cagcctgcgc
     2581 ttccagccgc tggcgcatca caccgctgcc catgatctcc agcgtcgccg gcatctgctg
     2641 gacgacctgc ccgaacgctt ggagcaaata ttgcacgccc ttccactctt ccaggcggcc
     2701 ggagaaaaca aatcgcaccg gttcaatttg caagttgcgc gatcgctcgt tggcgttcca
     2761 caagttcaga tcgacgccgt tctccaccag ctgctcgaca cgcccgcgca cgcccgtcgg
     2821 aagcgcccgg cgcgtgcgat cgttggccac gagcagcgtc tgcgcccgaa gtttgcccgg
     2881 catcagccaa ttgaacgcgt gcgaaatgag ccgccccacg cctacgaacg cccgcaccca
     2941 ccgcttctga atctgccgaa acgccggcgg atacgtgatg ccgccgttca tcggtccgat
     3001 gattaccggc gcccccacct tgtacatcag cgagatttcc ttcggcgaca ccggcgtcgg
     3061 ttggtgcacg atgtcgatgc ggtgcgcagc caccatctcc cgcacgatcc gccgagccgt
     3121 tcgcgcgctc accagccgcc cgataaagcg aaacaaaaag tgcttgatga ccggcggaaa
     3181 cagcagcgcg acgcggtcga gccgctgatg aaatcgcgtg ttcggcacga accgaacgcg
     3241 gtcgatgtcg ctggcgagca gcgtctccag ctcgccccgc gtccgctcgt tcacgacgag
     3301 ccacgcctcg atgccgcgct ggcgcagcag ccgaaaatag tgcaacggca gaatcgcctc
     3361 gccgccaaat ttcgccgagg cgtgctcagc gacgatgagg acgcgaatct ccggtccggt
     3421 ctctcggtaa gccgggagag agttagggtg agggtgattt gcgattggag aaacctgcac
     3481 gtcagcgctc aaccttcacg gtctccaact tcgccaccat cgcctccacc acctgatcgt
     3541 gcaccgcctg cagcggcccg ttcaccttca accccgccgc ccgcgcgtgc ccgccgccgc
     3601 cgaactgctc ggcgaatctc gccacatcca cgccgccctt actccgcaag ctcacgcgaa
     3661 tcgccccgcc gtccatcggc tccgtgaaca gcagcgacac ctcgaccgct cgaatctgca
     3721 gcgggatgtt gatcaggttt tccgtatcgc cgacgttggc gcgcaatgtc tcaaaatcgc
//
LOCUS       DATIMH010001048         6963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1118346, whole genome shotgun sequence.
ACCESSION   DATIMH010001048 DATIMH010000000
VERSION     DATIMH010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118346"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="VLI90_18190"
     CDS             complement(<1..1687)
                     /locus_tag="VLI90_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16198.1"
                     /translation="MRRWLLRILIGIAALIVLVVIGVQIVLWTNIPKNLVVKQIESEM
                     GLRISTDSLRTGWLGRSTLQNVALGLPLSDKSFLKVQTLQVKHTTLFGMIFGRAVSID
                     RIEIDHPEVFVRQDANGNWNLQEVAEILAKVSGGKTAQESQKQGIPRLPDVRLIEGVV
                     HVTDNGDKEATIAPLSIDGHGEGPLVWKFDVKLAKSVAINGEVAPGNTWKHQVRLSAH
                     DLGSLLNSWGVNSYGADLAGTWDGQLGEGGKLRGTFTVDHLKAKSIAAVGDVSVTGAI
                     DVDAGAGSAVTLRPRRIDLKTSNPALNNVWLAAGTITRDSTGIRAQTMQVAALGGLAV
                     LDASFDPRTFAGDLQLRWSGLAVSSGMNHSGSITAKLRMPFPGQPAINVDLLSQGATS
                     SGKWDAKVNVAGIGQSWKSIDWVITAPTLTYTGSHPIQMAQLVAHVTQRWPSVELADM
                     TIPERPHLQARAAADFSTHNWYFWLDAGGVPHPDNVPLPITFLINAWGNADVYTLHEL
                     DLQIADAIIWAGGYYDRRKPNPMNLDVVLTRSPQLAIDAPIQGHLGGRFNLSGR"
     gene            complement(1782..2543)
                     /locus_tag="VLI90_18195"
     CDS             complement(1782..2543)
                     /locus_tag="VLI90_18195"
                     /inference="COORDINATES: protein motif:HMM:NF042726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6655 family protein"
                     /protein_id="HSV16199.1"
                     /translation="MICQDDALLRRGSRRIPLLMWKLLLCFIAGGVGGCASIRVTDPQ
                     RSATEEFLENVATTMAVDKLSTAVLRDRKVYVETTFLTASTQPSNEHQYLLGELRSKL
                     LLNGVRLVDHREQAEIVMEVRSQGISIDRVEFLLGLPASSVGGIFTGGVAAATPELSI
                     IKTTKQHGFASVAYIAYWADSGEVVASSGPFVGYTIRDDWWFFGWGPRTIGNVPTALP
                     PPAETSTTAPPAKPPPSEKMTPVEGYKGKGPAAGK"
     gene            complement(2739..3614)
                     /locus_tag="VLI90_18200"
     CDS             complement(2739..3614)
                     /locus_tag="VLI90_18200"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSV16200.1"
                     /translation="MAELLQDWLRRAYPKASQTTLRRMLQDGRVRVNGSVARQMKRAI
                     EAGDDVQVASRTPTSEKPIAPLHPLALVLEDEHILVIDKPAGLLTSTVPRERRPTALA
                     IVRAYVKAREPSARVGLIHRLDRDASGLLIFSKTDQAYRSLKQQFFEHSVRRVYAAIT
                     HGVPTPSSGMIDSRLVELPDGSVRTSHRPDGGERAITHYEAIARERGLAALRVTLQTG
                     RKHQIRAHLSERGVPIVGDRVYGKGDEANRLMLAAVQLGFVHPQSKKEILCEIAVPRA
                     FPLVGGTKLSSMESK"
     gene            complement(4219..5379)
                     /locus_tag="VLI90_18205"
     CDS             complement(4219..5379)
                     /locus_tag="VLI90_18205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV16201.1"
                     /translation="MIGTFGHWKAGRDDPMQVAVTYSGQFLELCRELDADGYVISYNP
                     RRAKVVDGRFTIEHRPHRWKRGPGPLYHLGQIWYGVRLAVTAARFGADAAVVTDGTHW
                     FALGLMRLFGVRVIPSVHCVLWRHNRPPTDFTGKLVRRLNTRFFGRRASAVLCVSEAI
                     AQQIRPMMNGRATAAPIIPFLPTYRRESFGDGFPAPPPAPPFRVFYAGRIERNKGVFD
                     LLEIAKQLARDGQIDIDFDLCGTGSALEPLREAAAAAGVAERFRCHGHSPQSFMREMY
                     QRCHVVIVPTTSDFVEGFNKVVAEGVLAGRPVITSSVCPALEYVKDAVLEVPPDDAAG
                     YAAGILQLRDDKALYDSKQSASAVTRTQFYDPTFGWQAAVRRAIGKDPAQSH"
     gene            complement(5599..6270)
                     /locus_tag="VLI90_18210"
     CDS             complement(5599..6270)
                     /locus_tag="VLI90_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16202.1"
                     /translation="MIVRINQIDVAAAESATGWDPGPPRGGIDFTWPLRTFAYQIFIL
                     ETDERQQPLSAAFRQDQIRQLIPQVLGALAGDETQETVLRLDGPVSDGELLAAFGYAA
                     DPTGCERYAISQTHKLDPLPDAVLSSIRLVPTPALLKTMCADVKLGLDRAVRLRAFAV
                     PEPMINPLLDTTDLDDERWRDILPRATMVLSTAVSLQSLVVWTPRFDPTEAKRRIMQR
                     LTGRA"
     gene            complement(6288..6749)
                     /locus_tag="VLI90_18215"
     CDS             complement(6288..6749)
                     /locus_tag="VLI90_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16203.1"
                     /translation="MPSSTDFPARGRVIAVDDNAVIFAPSNTTYELKLLTSGRYDGPV
                     NTLIDGFVRGTARKLWTVASGGNFLTPIQGPTRIVQGRIKYLDNQVMVVQAGAPVIVD
                     LPRADTSYDLNNGPLAVGALVNCTILPGASFALANAAPAAGAGAAAAAAVV"
BASE COUNT         1249 a         2357 c         2094 g         1263 t
ORIGIN      
        1 accggccgga gagattgaat cgtccaccaa ggtggccctg gatcggcgca tcgatcgcca
       61 gctgcggcga gcgcgtcagc acgacatcca ggttcatcgg attgggcttt cgccgatcgt
      121 agtacccacc ggcccagatg atggcgtcgg cgatctgcag gtccagctcg tgcagcgtgt
      181 agacatccgc attgccccag gcgttaatca agaacgtgat gggcagcggc acgttatcgg
      241 gatgcggcac accgccggcg tcgagccaga agtaccagtt gtgcgtcgag aaatccgccg
      301 cagcgcgcgc ctgcaggtgc gggcgctcgg ggatcgtcat gtcggccagc tcgacgctgg
      361 gccagcgctg ggtgacgtgc gccaccagct gcgccatctg aatcggatgg ctgccggtgt
      421 acgtcagcgt cggcgcggtg atgacccagt cgatcgactt ccaggactgc ccgatgcccg
      481 cgacgttcac cttcgcgtcc cacttgccgg atgacgtcgc cccttggctg agcagatcga
      541 cgttgatggc cggttgaccg gggaacggca tgcgcagctt ggcggtgatc gacccgctgt
      601 ggttcatgcc gctgctgacg gccagaccgc tccagcgcag ctgaagatcg ccggcgaacg
      661 tgcgcggatc gaagctggca tcgagcaccg ccaatccgcc aagcgccgcc acctgcatcg
      721 tctgcgcgcg aatcccggtg ctgtcgcgcg tgatcgtccc ggcggcaagc cagacgttgt
      781 tcagcgcggg gttgctcgtc ttcagatcga tccgccgcgg ccgcagcgtg acggctgaac
      841 cggcgccggc gtcaacgtca atcgcgcccg tgacgctcac gtcgcccacc gcggcgatcg
      901 acttggcttt caggtgatcg acggtgaacg tgcccctcaa cttcccgcct tcgcccagtt
      961 gcccgtccca cgtcccggca aggtcagcgc cgtaggagtt gacgccccaa ctgttcaaca
     1021 gcgagcccag atcgtgcgcg ctcaggcgga cctggtgctt ccacgtgttg ccgggcgcga
     1081 cctcgccgtt gatcgcgacg gacttggcca gcttcacgtc gaacttccac acaagggggc
     1141 cctccccgtg accgtcgatg ctcagtggcg cgatcgtcgc ctctttgtcg ccgttgtcgg
     1201 tgacgtgaac cacgccctcg atcaagcgca cgtccggcag cctggggatg ccttgcttct
     1261 gtgattcctg cgcggtcttc ccgccgctca cttttgccag gatctccgcc acttcctgga
     1321 ggttccagtt gccgttggcg tcctggcgga cgaagacctc cggatgatcg atctcgatgc
     1381 gatcgatcga caccgcccgg ccgaagatca tgccgaacaa cgtcgtgtgc ttcacctgca
     1441 gcgtctggac tttgagaaac gacttgtcag acagcggcaa gcccagcgcg acgttctgca
     1501 gcgtcgatcg acccagccaa ccggtgcgca gcgagtcggt gctgatgcgc aggcccatct
     1561 cgctttcgat ctgcttgacg accaggtttt tgggaatgtt cgtccaaagg acgatctgca
     1621 cgccgatcac gacaagcacg atcaacgcgg cgatgccgat gaggatgcgc agcagccaac
     1681 gacgcatggt ggttctgggg aacgatgatc gatgaacgat gagcaatgac gtgcgtcgga
     1741 cgcagcggat tctatatcat cgttcagcgt tcagcgttcg actacttccc cgccgctggg
     1801 cctttgcctt tgtagccttc caccggggtc atcttctccg acggcggcgg tttcgccggt
     1861 ggggcggtgg tgctggtttc cgccggcggg ggtagggcgg tcggaacatt gccgatcgtc
     1921 cgcgggcccc agccgaagaa ccaccagtca tcgcgaatcg tgtacccgac gaacggcccg
     1981 gagctggcga caacctcgcc gctgtccgcc caatacgcga tgtacgccac gctggcgaat
     2041 ccgtgctgct tggttgtctt gatgatcgac agttccggcg tcgccgccgc caccccgccg
     2101 gtgaagatcc caccgacgct cgacgccggc aggcccagca gaaattcaac gcgatcgatg
     2161 ctgatcccct ggctgcggac ttccatcacg atctccgcct gctcgcgatg gtccaccagc
     2221 cgaacaccgt tgagcagcag cttcgatcgc aattcgccga gcaaatattg atgctcgttc
     2281 gacggctgcg tgctggcggt caggaaggtg gtctcgacgt acaccttgcg atcgcgcagc
     2341 accgcggtcg agagcttgtc gaccgccatc gtggtggcga cgttctccag aaactcctcg
     2401 gttgccgagc gctgcggatc ggtcacgcga atgctggcgc acccacccac gccgccggcg
     2461 atgaagcaca gcagcagctt ccacatcagc agcgggattc ggcgcgatcc gcggcgtaga
     2521 agtgcgtcgt cttggcagat caagtgcagt ccatccgtcc taaaagtgcg gctgcagcaa
     2581 tgtaagcgga tcggaagaaa tgaacaatcg gtaatcgagc gcacccgcgc gccttcaccc
     2641 caaaaccgac gccggtgtct gaggtactcc gaagaccggg ttcttcgtgc aatcgggccc
     2701 gggtcttcgg agtacgtcag acaccggcgt ccgacaacct atttcgattc catgctcgac
     2761 aacttcgttc cccccaccag cggaaacgcg cgcggcaccg cgatttcaca aaggatctct
     2821 ttcttgctct gcggatgcac aaaccccaac tgcaccgccg ccagcatcag gcgattcgct
     2881 tcgtcgccct tgccatacac gcggtccccg acgatcggca cgccgcgttc gctcaggtgg
     2941 gcgcggatct ggtgcttgcg gcccgtctgc agtgtcacgc gcagcgccgc caagccgcgc
     3001 tcgcgcgcga tcgcttcgta atgcgtgatc gcgcgctcgc ctccatcggg gcggtgggac
     3061 gtgcggaccg atccatcggg cagctcgacc aatcgcgagt cgatcatccc gctgctcggc
     3121 gtcggcacgc cgtgcgtgat cgccgcgtac acccggcgga cgctgtgctc gaagaactgc
     3181 tgcttgagcg agcgatacgc ctggtcggtc ttggaaaaga tcagcaaccc cgatgcatcg
     3241 cggtcgaggc ggtggatcag cccaacccgc gccgacggct cgcgcgcctt cacgtacgcc
     3301 cgtacgatcg ccagcgccgt cggccgccgc tcgcgcggca cggtgctcgt cagcagcccc
     3361 gcgggcttat cgatcaccaa gatatgctcg tcctcaagta ccagcgccag cggatgcagc
     3421 ggcgcgatcg gcttttccga agtgggcgtg cgcgaggcga cctgcacgtc atcccccgct
     3481 tcgatcgcgc gcttcatctg cctggcgacc gaaccgttca cgcgcacgcg gccgtcctgc
     3541 aacatgcggc gcagcgttgt ctggctcgcc ttcgggtaag cccgtcgcaa ccaatcttga
     3601 agtagctcgg ccatcgtgat gtgcgcgcca ccatagcgac acgagcgcgc aaaaaaagac
     3661 cccggtcatc acccacgatc gaccggagtc tcgatattct gccgcgctgc tgcgcgccgc
     3721 gcgccgcgcg cgttgcgtga agccaaccag acccttagcc atgcttcgcc gctggcgaag
     3781 acccacatgg ccctgcgggg tcggtgcccg cacagcgcac cgcaaccccg gaagcttccg
     3841 tccttacccc atctttgagc tcgtcccaag cattgcgacg tgcacgaaat gcgggccttc
     3901 tcacccacaa tcgccatcca aatactgtcg ggctgctccc ccgactcagt cttggacggc
     3961 atcttcattc gcttgcgacg tgacctgcaa agctgttgaa gatgcgggcg agtatgcggg
     4021 gttcgcccgg catcatcaag cctattttta tcaggaatca cccctaaaaa ctgcgcaaac
     4081 tgacagttgg gccaattttc ctgttgccat gcgaacaaat tgtaaaaatg ggtagttttc
     4141 gccttggcgc gttaatttgg ggcgtatgcc ctgatgaaaa tacaaagagg gcgctcgagg
     4201 accgggagta caggcaactt agtgcgattg ggcggggtct tttccgatcg cgcgccgtac
     4261 cgccgcctgc catccgaagg ttggatcgta gaactgcgtt cgcgtcactg cgcttgcaga
     4321 ttgctttgaa tcataaagtg ctttgtcatc tcgcagctga agaatccccg ccgcgtaccc
     4381 cgcggcatca tcgggaggca cctccagcac cgcgtctttc acgtactcca atgccgggca
     4441 caccgacgac gtgatcaccg gtcgccccgc cagcaccccc tcggccacca ccttgttgaa
     4501 cccctccacg aagtccgacg tcgtcggcac gatcacgacg tggcagcgct gatacatctc
     4561 ccgcatgaac gattgcgggc tgtgcccgtg acagcgaaac cgctccgcca cccccgcggc
     4621 cgccgctgct tcgcgcagcg gctccagggc gctgccggtg ccgcataagt cgaagtcgat
     4681 gtcgatctgc ccgtcgcgcg cgagctgctt ggcgatctcc agcaagtcga acacgccttt
     4741 gttccgctcg attcgcccgg cataaaagac ccgaaacggc ggcgccggtg gtggggcggg
     4801 aaacccgtcg ccgaacgact cgcgccgata tgtcggcagg aatggaatga tcggcgcagc
     4861 cgttgcgcgc ccattcatca tcgggcggat ctgctgcgcg atcgcctccg acacgcacag
     4921 cactgcactg gcgcgccgcc cgaagaagcg cgtgttcaat cggcgaacaa gcttgcccgt
     4981 gaaatctgtc ggcggacgat tgtgccgcca gagcacgcag tgcaccgacg ggatcacgcg
     5041 cacgccgaat agccgcatga gaccgagcgc gaaccaatgc gtcccatcag tgacgacggc
     5101 ggcatccgcg ccgaagcgcg ccgcggtgac cgccagccga acgccgtacc agatttgccc
     5161 caggtgatac agcggccctg gcccgcgctt ccaccgatgc gggcgatgct cgatcgtgaa
     5221 ccgcccgtcc accaccttcg cccgccgcgg gttgtaagag atcacatacc catcggcgtc
     5281 cagctcgcgg cacagctcca ggaactgccc ggaatacgtg accgccacct gcatcgggtc
     5341 atcgcgcccg gctttccaat ggccaaaggt cccgatcaca tcaccgggcc cggcggcgta
     5401 aagtaggcga agaggacgcg atttagccat tacgtttagc ggcctcgttt acccggcgcg
     5461 tttgtcttac cccctcgccc ggtactccgg gagagggtcg gggtgagggt gatttcggag
     5521 tatcgaacga ctttcgaaat acaaagtcac cctcacccta accctctccc ggactaccgg
     5581 gaccagagga gaagctcctc acgctcttcc agtcagccgc tgcatgatcc gccgcttcgc
     5641 ttcggtcggg tcgaacctcg gcgtccacac gaccagcgac tgcaggctca ccgccgtcga
     5701 caacaccatc gtcgcccgcg gaaggatatc gcgccagcgc tcgtcatcca gatcggtcgt
     5761 gtccagcagc gggttgatca tcggctcggg caccgcaaac gcccgcaggc ggacggcgcg
     5821 gtccaggccg agcttcacat ctgcgcacat cgtcttcaac agcgccggcg tcggaaccag
     5881 acgaatgctg ctcaataccg cgtccggcag cgggtcgagc ttgtgcgtct ggctgatcgc
     5941 ataccgctcg catcctgtcg gatcggcggc gtaaccaaac gcggcgagca gctcgccgtc
     6001 gctcaccggg ccgtccaggc gaagcaccgt ctcctgcgtc tcatcgccgg ccagcgcgcc
     6061 cagcacctgc gggatcagtt ggcggatctg gtcctggcga aacgcggcgg acagcggctg
     6121 ctgccgctcg tcggtctcga ggatgaatat ctgatacgcg aacgtccgca gcggccaggt
     6181 gaagtcgatg ccgccgcgcg gcgggcccgg gtcccagccc gtggccgact cggctgccgc
     6241 gacgtcgatc tggttgatcc gaacgatcat gaccgcagcg ctcgcgctta aacaaccgcg
     6301 gcggcagcag cggcaccggc accggcggcg ggagccgcgt tcgcgagcgc gaagctcgcc
     6361 cccggcagga tcgtgcagtt caccagcgcg ccaaccgcca gcggcccatt gttcaaatcg
     6421 tacgatgtat ccgcccgtgg cagatcgacg atcaccggcg cacccgcctg aacgaccatc
     6481 acttggttgt ccagatattt aatccgcccc tgcacgatgc gcgtcggccc ctggatcggc
     6541 gtcaaaaaat tcccgccact ggcgacggtc caaagcttgc gcgcggtccc gcgaacgaac
     6601 ccgtcgatca gcgtgttcac cggcccatcg taccgcccgc tggtcagcag cttgagttcg
     6661 taggtggtgt tcgacggcgc gaagatgacg gcgttatcgt ccacggcaat cacccgcccg
     6721 cgggcgggga aatcggtact gctgggcatg tcgcaagact cctgtagatc gcggcggaca
     6781 actttagcgg actcgcgccc agaggcaaat gcgctcaacg ttctctattt tgcgtcagta
     6841 gtgtccggtc agaagtcgaa tagcttcaac tggttgcgat tttcgtcttc ggcgattgtt
     6901 tgtccgatct ccaaaaacgc ctgatttacc ggcgttttct cgaacatcat gacgctgagg
     6961 att
//
LOCUS       DATIMH010001049         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1409508, whole genome shotgun sequence.
ACCESSION   DATIMH010001049 DATIMH010000000
VERSION     DATIMH010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409508"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..971
                     /locus_tag="VLI90_18220"
     CDS             78..971
                     /locus_tag="VLI90_18220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HSV16204.1"
                     /translation="MSQQAARFHESPARSRAAASSAFTLVELLVVIGIIAILIGVLLP
                     ALNSARRQANQVACQANLHQLGLALIMYNTETKYYPGDINTANGQTYAVWPTRLRRYM
                     KGNQAVFRCPSLARDFDWKVNDTTPPVALIADTGFGYNQGESLLVRNVAKFGYGYNDW
                     GSYQQPTSTGTGTIYGDGNPGPNDRQRGLGGDVNTSYGKELKATRVYRPTEMIAITDI
                     KVPIGANFCFNVDPNDPNEAPSDIHKGGSNVLWCDGHVTWKHQKELCLYDLKSLQSFF
                     NFKTSQLWLNNAPQWNNDHRP"
     gene            1022..>1681
                     /locus_tag="VLI90_18225"
     CDS             1022..>1681
                     /locus_tag="VLI90_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16205.1"
                     /translation="MALRAPAQVGQGPRFEPAIWRYTLNRPAEGWQAPGFDDVDWRTG
                     RGAFGTAGTPGITINSNWSTADIWLRRAFVLPTTALDVPATQLLVFHDEDVEIYFDGV
                     LAARAAGFVRDYEPMEILPEARRLLKPGGSITVAVHCHQTIGGQGIDVGLTKVSSEWL
                     AVRRRNAYRAFALNNTGNAAAGRRLFLDEQRVGCIRCHTIDGSASRAGPDLQTIGDKF
                     VR"
BASE COUNT          320 a          526 c          543 g          292 t
ORIGIN      
        1 ggtggcggct cgcatcccct gaccggcggg ccgccacccc ccttttaggg cgcttacaaa
       61 cgcccgggag gcttgtcatg agccaacaag cggcacggtt tcacgaatca cccgcgcgat
      121 cgcgcgccgc ggcatcgtcg gcgtttacgc tggtcgagtt gctcgtggtg atcggcatca
      181 tcgcgatcct gatcggtgtc ctcctgcccg cgctcaattc cgcgcggcgc caggcgaacc
      241 aggtggcttg ccaagccaat ctgcaccagc tggggctggc gctgatcatg tacaacacgg
      301 aaacgaagta ctatcccggc gacatcaaca ccgccaacgg ccagacctat gccgtctggc
      361 ccacgcgcct gcggcggtac atgaagggaa accaagcggt gttccgttgc ccgtcactgg
      421 cgcgcgattt cgactggaag gtgaacgata cgacgccgcc ggtggccctc atcgccgata
      481 cagggttcgg ttacaaccag ggcgaaagcc tgctggtgcg gaacgtggcg aagttcggtt
      541 acggctacaa cgactggggt tcgtaccagc agcccaccag caccggcacc ggcacgatct
      601 atggagatgg gaacccgggg ccaaacgacc ggcagcgcgg cttgggtggc gatgtgaaca
      661 ccagctacgg caaggaactg aaggcgaccc gcgtgtatcg gccgactgag atgatcgcga
      721 tcaccgacat caaggtgccg atcggcgcga acttctgctt caacgtcgat cctaacgatc
      781 cgaatgaagc gccgtccgac atacacaagg gcggcagcaa cgtgctttgg tgtgacggcc
      841 acgtgacgtg gaagcaccag aaggaactgt gcctgtacga cctgaagtca ctccagtcgt
      901 tcttcaactt caagaccagc cagctgtggt tgaacaacgc cccgcaatgg aacaatgacc
      961 accggccgtg atgcgaccga tgcgctgctc agtcgtcgtc acttgcatat tcgctttgta
     1021 tatggcgctc cgcgcaccgg cccaggttgg gcaggggccg cggttcgagc cggccatttg
     1081 gcgttataca ttgaaccgac cggcggaagg gtggcaggcg cctggcttcg acgacgtcga
     1141 ctggcgcacc ggccgcggtg cattcggcac cgctggcacg ccgggcatca ccatcaacag
     1201 caactggtcc accgccgaca tctggctccg ccgagcgttt gtccttccaa ccacagcgct
     1261 cgatgtgccg gcaacgcagc tgctggtgtt tcacgacgag gatgtggaga tctattttga
     1321 cggcgtgctg gccgcgcggg cggcggggtt cgtcagggat tacgagccga tggagatcct
     1381 gcccgaggcc aggcggctgc tgaagccggg cggttcgatc accgtggcgg tgcattgcca
     1441 tcagaccatc ggcgggcagg ggatcgatgt gggactgacc aaggtatcga gcgaatggct
     1501 ggccgtgcgc cggcggaacg cctatcgggc cttcgcgctg aacaacaccg gcaacgccgc
     1561 cgcgggtcgt cggctttttc tggacgagca gcgcgtcggg tgcattcgct gtcacacgat
     1621 cgacggcagc gcgagtcgcg cgggtccaga tctgcagacg atcggcgata agttcgtccg
     1681 c
//
LOCUS       DATIMH010001050         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4294658, whole genome shotgun sequence.
ACCESSION   DATIMH010001050 DATIMH010000000
VERSION     DATIMH010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4294658"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VLI90_18230"
     CDS             <1..1049
                     /locus_tag="VLI90_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16206.1"
                     /translation="PASTPVSTDARLRTAHRVFRGALIFNTALTAFWLFALLTHRTGG
                     FFTNYEIGRDVLGRIAGGILFFYVVWGFIWYGIKSLLLKHFVGFTKDERRQAFSSRMR
                     EPFEVSALTSRYSERRIRIVDMIGRRGRFITLAAAGFFYLYARVSVDPSPGFATLFLQ
                     DNLLDAVITSWVFLGFFYADGTLAAAFYGPQSRVMDGVLARANCLLITTLWTAFKFVM
                     VPLGSRLAVVFPSNRFAAVFALIWGAYIIGDAMAEIGGALFGKQTLRVRGIGDVNRKS
                     IGGTVSGFAGALALCLWVVISQGMPPAWIAVAVIIAAANTLIELFSPRGTDDFFMATG
                     NALICLVFGMLLPA"
     gene            1170..>2235
                     /locus_tag="VLI90_18235"
     CDS             1170..>2235
                     /locus_tag="VLI90_18235"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSV16207.1"
                     /translation="MQTIGRYELLEKLGQGGMGAVYRARDTLLQRIVAVKVIATSIDQ
                     NAELRERFFREARAAGQLSHKNIITIHDLGEHEGQPYLAMEYLQGQDLLARMASPQRM
                     SLRRRVEIAIEICEGLDFAHVHGVVHRDIKPANIFITDAGVVKILDFGLARLVTSELT
                     RSNMMMGTINYMSPEQIRGEKIDHRADVFSTAVVLYELLSGRRAFDADSFAATLYKIL
                     QEFPEPLQQIDPTIPSELVHIVERGLAKPRDERYQHMSEMLLDLAVFRQQLAGMDSPA
                     GGRLAPTELRTPSDRVFPVTPPIPLPPVATPLPLTGPSTPAPAPVASNPPAPAGVRSY
                     ILPLIAVLALAIAAIAMWNSS"
BASE COUNT          343 a          726 c          704 g          462 t
ORIGIN      
        1 ggcctgcgtc cacccccgtt tccacggacg cacgcctgcg cacagcacat cgtgtttttc
       61 gcggtgcgct gatcttcaat acggcgctga ccgccttctg gctgtttgcg ctgctgacgc
      121 accgcaccgg cggatttttc acaaactacg aaatcggtcg cgacgtgctc gggcgtatcg
      181 ccggcgggat tctctttttc tatgtggtgt ggggcttcat ctggtacggg atcaagagtc
      241 tcctgctgaa gcacttcgtt ggctttacga aggacgagcg gcgccaggcc ttttcgtcac
      301 gaatgcgcga accattcgag gtgtcggcgc tgacctcgcg gtattcggaa cgtcgcatcc
      361 gcatcgtcga catgatcggc cggcgcggcc ggttcattac ccttgctgcg gcggggttct
      421 tctatctcta cgcgcgcgtc tcggttgatc cgtcgcctgg atttgccacg ttgttcctgc
      481 aggacaacct gctcgatgcg gtgatcacca gctgggtgtt cctggggttc ttctatgccg
      541 atggcaccct ggcggcggcg ttttacggac cccagtcgcg ggtcatggac ggcgtcctcg
      601 cgcgcgccaa ctgcctgctg atcacgacgc tgtggacggc gttcaagttc gtcatggttc
      661 cactcggcag ccgcctcgcg gtcgtgttcc cgtcgaaccg cttcgccgcg gtgttcgcgc
      721 tgatctgggg tgcctatatc atcggcgacg cgatggccga gatcggcggc gccctgtttg
      781 gtaaacagac gctgcgcgtg cgcggcatcg gcgacgtgaa tcgaaaatcg atcggcggca
      841 cggtatccgg tttcgccgga gcgctcgcgt tgtgcctgtg ggtggtcatc agccagggca
      901 tgccgccggc gtggatcgcg gtcgcggtga tcatcgccgc cgccaacacg ctgatcgaac
      961 tgttctcgcc gcgcggaacc gacgacttct ttatggcgac cggcaacgcc ttgatctgcc
     1021 tggtgttcgg gatgctgctg cccgcctgac ggcccgtttc gacagtactc ccgtccctat
     1081 cctttggtcc tcgcggcact tgtgggcgcc ggagggcact gctatcatcc cgccttacct
     1141 tgcccgggta gtccccgggc gcctctctca tgcaaaccat cggtcgctac gaactgcttg
     1201 aaaaactcgg ccagggtggc atgggtgcgg tgtatcgcgc gcgtgacacg ctgctgcagc
     1261 ggattgtggc ggtcaaggtg atcgccacct cgatcgatca gaatgcggag cttcgcgagc
     1321 gattttttcg cgaggcgcga gccgccggtc agctctcgca caagaacatc atcacgattc
     1381 acgatctggg cgagcacgag gggcagccgt acctggcgat ggagtacctc cagggacaag
     1441 acctccttgc gcgcatggcg tcgccgcagc gcatgagcct gcgccgccgc gtcgagatcg
     1501 cgatcgagat ctgcgaaggg ctggatttcg cgcacgtgca cggcgtggtg catcgcgaca
     1561 tcaagccggc gaacatcttc atcaccgatg ccggggtcgt caagatcctc gatttcgggc
     1621 tggcgcggct ggtcacctcc gagctgacgc gcagcaacat gatgatgggc acgatcaact
     1681 acatgtcgcc cgagcagatc cgcggcgaga agatcgatca ccgggccgat gtcttttcga
     1741 ccgccgtggt gctctacgag ctgctgagcg gacgccgtgc gttcgatgcc gattcgtttg
     1801 ccgcgacgct ctacaagatc ctgcaggaat tcccggaacc actgcagcag atcgatccca
     1861 cgattccaag cgagctggtg cacattgtcg aacgcgggct ggcgaagccg cgcgacgagc
     1921 gctatcagca catgagcgag atgctgctcg acctggcagt gtttcggcag cagctcgcgg
     1981 gcatggattc ccccgccggt ggccgcctcg cgccgacgga gttgcgaacc ccctcggatc
     2041 gagtgtttcc cgtcacgccg ccaattccgc tgccgccggt ggcgacgccg ctgccactga
     2101 ccgggccatc gacgccggcg ccggctccgg tcgcatcgaa tcccccggcg cctgccggcg
     2161 tacgttcgta catcctgccg ctcatcgcgg tgctcgccct ggcgatcgcc gccatcgcga
     2221 tgtggaattc gtcgc
//
LOCUS       DATIMH010001051         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4294938, whole genome shotgun sequence.
ACCESSION   DATIMH010001051 DATIMH010000000
VERSION     DATIMH010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4294938"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VLI90_18240"
     CDS             <1..933
                     /locus_tag="VLI90_18240"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSV16208.1"
                     /translation="TANREPRTANREPRTANHCYMLRVMFKRQRTGSVVCASCGSLVG
                     VNDEQCYSCGRRNPGLWGFGPLLRHLGADFGFVPFVIYGCVTLYALSLLLTIKEGGNI
                     MGGGLFSMLAPGGYATMMLGVSGAYPVFAYGRWWTVLSAGWLHGSALHILFNMMWVRD
                     LGPPVAEMYGGARMVMIYTISSACGFLLSSTAGYLLPGVPFLGGAPYTLGASAAIFGL
                     LGALVHYGRRGGSSMIRTHAMSYAVSMFVFGLIMPGIDNYAHAGGFVGGYLTSVWLDP
                     LKPERVNHIVGALVCLGATVLAIVASIVSTYRLT"
     gene            complement(939..>2121)
                     /locus_tag="VLI90_18245"
     CDS             complement(939..>2121)
                     /locus_tag="VLI90_18245"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HSV16209.1"
                     /translation="TRTSHVMYSRGFASIYGEWETTGEQKTVHRTFHESLRFPWPREP
                     VTVSLAKRQPDNSFALIWTTEIDPRSRFVVSAPMKQHAGRVWPVFVNGPASTKVDLLV
                     IGEGYTAAELPKFHRDVNRLLPALFEQEPFKGRRSDFNVRALDLPSAESGINRPNASV
                     FRRTPLSTEYNIFDSERYVLTLDNRALRDAASAAPYEFIEILVNDKTYGGGGIFNDQA
                     TASVDSAFSPYVFVHEFGHHFAALADEYYTSDVAYESGAAVKPEPWEPNITALHDAEQ
                     LKWKDLVTPGTPLPTPWNKDEYERHTAGIQQRRRDIRARNAPEAEMDALFREQQAWDA
                     KFLSSMTYSGRVGAFEGAGYEARGLYRPEADCIMFTRDPVGFCRVCRRAITRIIDLYS
                     R"
BASE COUNT          362 a          639 c          675 g          445 t
ORIGIN      
        1 accgcgaacc gcgaaccgcg aaccgcgaac cgcgaaccgc gaaccgcgaa ccattgttat
       61 atgcttcgag tgatgttcaa gcggcagaga accggatcag tggtgtgcgc ctcgtgcggc
      121 tcgctggtcg gcgtgaacga cgagcaatgc tactcgtgcg gccgccgcaa ccctggcctg
      181 tggggttttg gtccgctgct gcgtcatctt ggcgccgact tcggttttgt gccgtttgtc
      241 atctacggct gtgtgacttt gtacgcgttg agcctcctcc tcaccataaa ggaaggaggc
      301 aacatcatgg gcggcggcct gttctcgatg ctcgcgccgg gtggatacgc gacgatgatg
      361 ctcggggtca gcggcgctta tccggtattc gcctacggcc gctggtggac cgtgctcagt
      421 gccggctggt tgcacggcag cgcgctgcac attctcttca acatgatgtg ggtgcgcgac
      481 cttggcccgc cggtagcgga gatgtacggc ggcgcgcgga tggtgatgat ctacaccatc
      541 tcgtccgcat gcggattcct tctgagttcg actgccggct atctcctgcc gggcgttccg
      601 tttctcggcg gtgcgcccta caccctggga gcctcagccg cgatctttgg attgctcgga
      661 gcgctcgttc actatggacg ccgcggcggc agcagcatga tccgcacgca tgcgatgagt
      721 tatgccgtgt cgatgtttgt cttcggactg atcatgccgg gcatcgacaa ctacgcgcat
      781 gccggcgggt tcgttggcgg atatctcacc agcgtgtggc tcgatccgtt gaagcccgaa
      841 cgagtcaacc acatcgtcgg cgcgctggtg tgcctggggg cgacggtgct cgcgattgtc
      901 gcgtcgatcg tgtcgaccta ccgcctgacg taacgggcct accgcgagta gagatcgatg
      961 atccgcgtga tcgcacggcg gcacacgcgg cagaacccga ccggatctct cgtgaacatg
     1021 atgcaatccg cttcggggcg atagaggccg cgcgcctcgt atccagcccc ctcgaacgca
     1081 ccgacccggc ctgagtaggt catcgaggac agaaacttcg catcccatgc ctgctgttcg
     1141 cggaagagcg catccatctc cgcttcgggt gcgttgcgcg cgcgaatgtc gcgacggcgt
     1201 tgctggatgc cggcggtgtg tcgctcgtac tcgtctttgt tccacggcgt tggcagggga
     1261 gtacccggcg tgacgagatc cttccatttc aactgttccg catcgtggag cgccgtgatg
     1321 ttgggctccc atggctccgg cttcacggct gcaccgcttt catacgccac gtcagacgtg
     1381 tagtactcgt cggcgagcgc tgcgaagtga tggccgaatt cgtggacgaa gacatacggc
     1441 gaaaatgcgc tgtcgaccga cgccgtcgcc tggtcgttga agattcctcc gccaccgtag
     1501 gtcttgtcgt tgacgaggat ctcgatgaat tcgtacggcg ccgccgaggc ggcgtcgcgc
     1561 agcgcacgat tgtcgagcgt caggacgtag cgctccgaat cgaagatgtt gtattcggtg
     1621 gacagcggcg ttcgtcgaaa cacgctggca ttgggacggt tgattccgct ctcggcggat
     1681 gggagatcaa gcgcgcgcac attgaaatcc gaccgacggc ccttgaatgg ctcctgctcg
     1741 aaaagcgccg ggagcagccg gtttacgtca cgatggaatt tcggcaactc ggcggcggtg
     1801 tatccctcgc cgatgaccag cagatccacc ttggtggacg ccggaccgtt gacgaacacc
     1861 ggccacacgc gcccggcgtg ctgtttcatc ggagcggaga cgacaaagcg cgatcgcgga
     1921 tcgatttcag tagtccagat caaagcgaag ctgttgtccg gctgcctttt cgccagcgag
     1981 accgtaaccg gttcccgcgg ccatggaaac cgcagcgatt cgtgaaacgt gcgatggacg
     2041 gttttctgtt cgcctgtcgt ttcccattcg ccgtagattg acgcaaagcc ccgcgaatac
     2101 atgacgtgtg acgtccgcgt g
//
LOCUS       DATIMH010001052         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2271411, whole genome shotgun sequence.
ACCESSION   DATIMH010001052 DATIMH010000000
VERSION     DATIMH010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271411"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..>1819
                     /locus_tag="VLI90_18250"
     CDS             110..>1819
                     /locus_tag="VLI90_18250"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HSV16210.1"
                     /translation="MPLDSKSPAGPIETRWDRHRFSLKLVNPANKRKHTIIVVGTGLA
                     GGAGAATLAELGYNVKCFCFQDSPRRAHSIAAQGGINAAKNYHNDGDSVYRLFYDTVK
                     GGDFRARESNVYRLAQISLNIIDQCVGQGVPFAREYSGYLDNRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQSLERQIGAGRVQMFPRTEMLDLIVIDGRARGIVTRDLITGKIESHI
                     GDAVVLGTGGYGNVYYLSTNAKGSNATAIWRAHKRGALFANPCFTQIHPTCIPVSGEY
                     QSKLTLMSESLRNDGRVWVPLKKGDKRPPNQIPPAERDYYLERKYPSFGNLVPRDVAS
                     RNAKQVCDEGRGVGETGLAVYLDFADAIQRLGKPVIKERYGNLFDMYERITGEDPYQV
                     PMRIFPAIHYTMGGLWVDYNLMSNVPGLYVVGEANFSDHGANRLGASALMQGLGDGYF
                     VLPNTINDYLASNKFPAADSSHAAVREAEANVANTTKKLLETKGTRSVDSYHRELGKI
                     MWEYCGMGRTAEGLKTALSQIPGLREQFWRDVKVLGAGEELNQSLEKAGRVADFFELA
                     ELIC"
BASE COUNT          359 a          595 c          552 g          313 t
ORIGIN      
        1 ccgctgctca aaaagggcgc ggcgctcgtc gccattctga ttgccgtcgg caacatctcg
       61 attccgatcg cggtgttgac gggtttcttg actctgtaag gacgccagca tgccactcga
      121 ctcgaaatct ccggcgggcc cgatcgaaac gcgatgggac cgccaccgct tcagcctgaa
      181 gctggtgaat ccggccaaca agcgcaagca tacaatcatc gtcgtcggca ccggcctggc
      241 aggcggagcc ggcgcagcca ccttggccga actcggctac aacgtgaagt gcttctgctt
      301 ccaggattcg ccgcgccgcg cgcacagcat cgccgcgcag ggcggaatca acgccgccaa
      361 gaactatcac aatgacggtg acagcgtgta ccgcctcttt tacgacactg taaaaggcgg
      421 cgacttccgc gcgcgcgaat ccaacgtgta ccgcttggcg cagatcagcc tgaacatcat
      481 cgatcagtgc gtcgggcagg gcgttccctt cgcccgcgag tactccggct atctcgataa
      541 tcggagcttc ggcggcgcgc aggtgtcacg cacgttctat gcccgcggtc agacgggcca
      601 gcagttgctg ctcggcgcat accagtcgct ggaacgccag atcggcgccg gacgcgtgca
      661 gatgttccct cggaccgaga tgctcgacct gatcgtcatc gacggccggg cccgcggcat
      721 cgtcactcgc gacctgatta ccggcaagat cgaatcgcat atcggcgacg cggtcgtgct
      781 gggcaccggc gggtacggca acgtgtacta cctgtccacc aacgccaaag ggtcgaacgc
      841 cacggcgatc tggcgcgccc acaagcgcgg cgccctgttc gccaatccct gtttcaccca
      901 gatccatccc acctgcatcc cggtgtccgg cgagtatcaa agcaagctga cgctcatgag
      961 cgaatcgctg cgcaacgacg ggcgcgtctg ggtgccgttg aaaaagggcg acaagcggcc
     1021 gccgaatcag attcctccgg ccgagcgcga ttattacctg gagcggaaat atcccagctt
     1081 cggcaatctc gtgcctcgcg acgtggcatc gcgcaatgcc aagcaggtct gcgacgaagg
     1141 ccgcggcgtg ggcgagaccg ggctggccgt ctacctcgat ttcgccgatg ccatccagcg
     1201 cctcggcaaa ccggtcatca aagagcgcta cggcaatctg ttcgatatgt acgagcgcat
     1261 caccggcgag gacccctacc aggtcccgat gcgcatcttc ccggctatcc actacaccat
     1321 gggcggcctt tgggtggatt acaacctgat gtccaacgtg cccgggctgt acgtagtcgg
     1381 cgaggccaat ttctccgacc atggtgcgaa tcgcctgggc gccagcgcgc tcatgcaggg
     1441 tctgggcgac ggctacttcg tcctcccgaa caccatcaac gactacctgg catccaacaa
     1501 attcccggcg gcggacagtt cgcatgccgc ggtccgcgag gccgaggcca acgtcgccaa
     1561 cacgaccaaa aagctgctgg agaccaaggg cacgcgatcg gtggattcct accatcgcga
     1621 actgggcaag atcatgtggg aatactgcgg catgggtcgt acagccgagg ggttgaaaac
     1681 cgccctgtcg cagatacccg gcctgcgcga gcagttctgg cgcgacgtga aggtgctggg
     1741 cgccggcgag gagttgaacc agtcgctgga gaaggccggc cgcgtggccg acttcttcga
     1801 actggccgaa ctgatctgt
//
LOCUS       DATIMH010001053         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4000599, whole genome shotgun sequence.
ACCESSION   DATIMH010001053 DATIMH010000000
VERSION     DATIMH010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4000599"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VLI90_18255"
     CDS             complement(<1..537)
                     /locus_tag="VLI90_18255"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HSV16211.1"
                     /translation="MTFLGLDCGSSSIKAAILRDDRVAGKIVRASYPTHFDGVHAEVD
                     PQAVLRAVRDVVRQLGAAAGHVDAIVLDAMAASWVAMDRSGKPLTPIVTHQDRRSVEV
                     AMRIERDVGKARHLRLSGNRPIPGGISSTTWAWFNQRRPGVMRRADLVGHLSTFLHRA
                     LTGARVVDPSHASFMGVYS"
     gene            complement(625..864)
                     /locus_tag="VLI90_18260"
     CDS             complement(625..864)
                     /locus_tag="VLI90_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16212.1"
                     /translation="MTDPRDYKIEIAGASQPSSEAASSPRPFLSVRFACCGVYQRIYR
                     SVDGASYQGRCPRCGKPVKFLVGEGGTDARQFIVY"
     gene            complement(890..1516)
                     /locus_tag="VLI90_18265"
     CDS             complement(890..1516)
                     /locus_tag="VLI90_18265"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HSV16213.1"
                     /translation="MHDRTATIDQFLTATAARQPIPGGGSVAALAGALAAAIGEMVLS
                     YSIGKRDLASHEPQLKSIAHDLHRARDLLLQLMVEDQLAYAALSAIKKLPADSPERAD
                     RLPAAVVTCIRGPQAIGATAVAILELCDRCVEIVNHYLLSDLAVCAELAMATVRSAIY
                     NCRVNLVELSEPADRASIEQSMKELSSHGLQLIQRIVPRIWARHESSK"
     gene            complement(1624..>3494)
                     /locus_tag="VLI90_18270"
     CDS             complement(1624..>3494)
                     /locus_tag="VLI90_18270"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HSV16214.1"
                     /translation="QQLVSAIAAGDDTPSFADGTFTVQMQLGADKVDAATHAVLDALD
                     RLKREPIDEDRIRRAKTQLKVGRVKSLQTVEEISTSLADDFLSTGDPHFSDRYVDRID
                     KVTASDLQDVARRYLDRSRLITTLLLPAEAVGSEGLPKAEELIRPAAPTTQAGTKQVA
                     SDVKHVELDNNVVLLLKRISTSPLVVMNMYSLGGVTAEDEKTNGLGNLSMQMLSRGTT
                     SRNAEQIAEFFDSVGGELQCVCGNNSWYWTASCLKADFEKTLDAYSDVVNHPSFPQAE
                     FGPMKQRIDAAIDSEDADWTGQAFRYFKQEYFGPRNSPYRFTAVGTKANIDHFTTDQA
                     RQWYTQKVLTGRRVLAIYGDIDLQQAESLARKYFGDGAKVTAATPAHEQGAEPANNNS
                     PGISVNVFDVKVQPTQQALAGVVVGFDANPVIGDPVNFPLDVGQTMAGGWGYPTGYLF
                     ETLRGKGLVYVVQAQNNPGRSAATPGTFFVFAGCDPSKVNDVVEDILLNIARLQGTPA
                     DVNEQWFARSKLLITTADALENETPAQQATTEALDELFGLGYDYHRHFADKINAVTLD
                     QVRQIAAARLRQCMVTICTPDPNAVKINTGPRTYSTFPSVDLTPRGVQHDTGAARP"
BASE COUNT          609 a         1118 c         1106 g          661 t
ORIGIN      
        1 cgaatagacg cccatgaacg atgcgtgcga cggatcgacg acgcgcgcgc cggtgagcgc
       61 gcggtgcagg aacgtgctca ggtggccgac caggtccgcc cgccgcatca cgcccggtct
      121 gcgctggttg aaccacgccc acgtggtcga actgatgccg ccggggatcg ggcggttgcc
      181 gctcaggcgc aggtgccgcg ctttgccgac atcgcgctcg atgcgcatgg cgacttcgac
      241 gctacgccga tcctggtgcg tgacgatcgg cgtgagtggt ttgcctgatc gatccatcgc
      301 gacccacgac gccgccatcg cgtcgagcac gatggcgtca acgtgaccgg cagcggcgcc
      361 gagctggcgg acgacgtcgc gcacggcgcg cagcaccgcc tgcggatcga cctccgcgtg
      421 cacaccatcg aagtgcgtcg ggtatgaagc gcggacgatc ttccccgcga cgcggtcgtc
      481 gcgcaggatc gcggctttga tggatgatga accgcagtcg aggccgagga aggtcatgtt
      541 caggagtcag tcgtcagttg tccgtggtcc gttgtcggta ccgttacaag caactgacaa
      601 cggactacag gcaactgaca gtaatcagta aacgatgaac tgccgcgcgt cggtgccgcc
      661 ttcaccaact aaaaacttca ccggcttgcc acatcgcggg cagcggccct ggtagctggc
      721 gccgtcgacg ctgcgataga tgcgctgata gacgccgcaa caggcgaagc gaacgctcag
      781 gaatgggcga ggtgacgacg cagcctcgct cgatggctgc gaagcgcccg cgatttcgat
      841 cttgtaatcc ctcgggtccg tcatcgaagc tcgggccgtt ggcgcaacct catttgctcg
      901 attcgtgccg ggcccagatc cgcggcacga tgcgctggat cagctggagt ccgtgcgacg
      961 acagttcctt catcgactgt tcgatcgatg cgcgatcagc gggctcactg agctcgacca
     1021 gattgacacg gcagttgtag atcgcagacc tcacggtggc catcgccaat tccgcgcaca
     1081 cggcgaggtc ggacagcagg taatggttga cgatctcgac gcagcgatcg cacagttcca
     1141 ggatggcaac cgcggtggcg ccgatcgcct gcgggccgcg aatgcacgtg acgaccgcgg
     1201 ccggcaaccg gtcagcgcgc tccggcgaat cggccggcag cttcttgatc gcactcaacg
     1261 cggcgtaagc caactggtct tcgaccatca gctgaagcag caagtcgcgg gcgcggtgaa
     1321 ggtcatgcgc gatcgatttg agctgcggct cgtgcgaggc taaatcgcgc ttgccgatgc
     1381 tatagctcag gaccatctca ccgatcgccg ccgcgagcgc gccggccagc gcggccacgc
     1441 tgccaccgcc aggtatcggt tgccgggcgg cggtggcggt caagaactgg tcgatcgttg
     1501 cggtgcggtc gtgcatgggc atgcggttgt cggtcgcgat caatggattg tcaacggtga
     1561 caagcgatgg ggagagccgg cccgtccccg ggccggatcg ggccgaggac ggcccggctc
     1621 tccttatggc ctggccgcgc cggtgtcatg ctgcacgccc cgcggcgtca ggtcgaccga
     1681 tggaaacgtg ctgtacgttc gcggacccgt gttgatcttc acggcgttcg ggtcgggcgt
     1741 gcagatggtg accatgcact gccgcaggcg cgccgccgcg atctgccgca cctggtccag
     1801 cgtcaccgcg ttgattttgt cggcgaagtg ccggtggtag tcgtaaccga ggccgaacaa
     1861 ctcatccagc gcctccgtcg tcgcctgctg cgccggcgtc tcgttctcca gcgcgtctgc
     1921 cgtcgtaatc agcaacttcg atcgcgcaaa ccattgctcg ttcacatcgg ccggcgtgcc
     1981 ctgcaggcgg gcgatgttga gcaggatatc ttcgacgaca tcgttcactt tcgacggatc
     2041 acatccggcg aaaacgaaaa atgtccccgg cgtggccgcg ctgcggccgg ggttgttctg
     2101 ggcttgaaca acgtacacca gcccctttcc gcggagggtt tcgaagagat agcccgtggg
     2161 ataaccccac ccgccggcca tcgtctggcc gacgtcgagc gggaagttga cgggatcacc
     2221 gatgaccgga ttggcgtcga agccgacgac gacgccggcc agcgcctgtt gcgtcggctg
     2281 cacctttaca tcgaaaacgt tcaccgaaat gcccggcgag ttgttgttcg ccggttcagc
     2341 gccttgctcg tgagccggcg tcgcggcggt caccttcgcg ccatcgccga aatatttccg
     2401 cgccagcgac tccgcctgct gcagatcgat gtcgccataa attgcgagca cgcgccggcc
     2461 agtcagcacc ttctgcgtat accactgccg ggcctgatcg gtggtgaaat gatcgatgtt
     2521 ggccttcgtg ccgacggccg tgaagcgata cgggctgttc cgcggaccga aatattcctg
     2581 cttgaagtag cggaacgcct ggccggtcca gtcggcatct tcgctgtcga ttgccgcgtc
     2641 gatccgctgc ttcatcgggc cgaattcggc ttgcggaaac gacgggtgat tgacgacgtc
     2701 gctgtatgca tccagcgttt tctcgaaatc cgctttcagg cacgatgcgg tccagtacca
     2761 actgttatta ccacaaacac attgcaactc accgccgacg gagtcgaaga actccgcgat
     2821 ctgctcggcg tttcggctgg tggtcccgcg agacagcatc tgcatcgaca ggttgcccag
     2881 gccattggtc ttctcgtcct ccgccgtcac gccacccagg ctatacatgt tcatgacgac
     2941 cagcggtgag gtgctgatgc gcttgagcag caggacgacg ttgttgtcca gctcgacgtg
     3001 cttcacatcg ctggccactt gcttcgtccc cgcctgagtc gtcggggcgg cggggcggat
     3061 cagctcttcc gccttcggca gtccttcgct gccaaccgct tcggccggca gcagcagggt
     3121 ggtgatcaac cggctgcgat cgagataacg ccgggcaacg tcctgcagat cgctggccgt
     3181 caccttgtcg atccgatcga catatcgatc gctgaaatgc ggatcgccgg tgctgagaaa
     3241 gtcatcggcc agcgatgtcg aaatctcctc aaccgtttgc agggatttca cccggccgac
     3301 cttcagctgc gtcttcgccc gccgaatgcg atcttcatcg atcggctcgc gcttcaagcg
     3361 gtccagtgca tccaacaccg cgtgagttgc ggcgtccacc ttgtcggcgc ccaactgcat
     3421 ctgcaccgtg aacgtgccat ccgcgaatga tggcgtgtca tcaccggcgg cgatggcgct
     3481 gaccaactgc tgct
//
LOCUS       DATIMH010001054         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4295180, whole genome shotgun sequence.
ACCESSION   DATIMH010001054 DATIMH010000000
VERSION     DATIMH010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4295180"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /gene="yidC"
                     /locus_tag="VLI90_18275"
     CDS             complement(<1..1001)
                     /gene="yidC"
                     /locus_tag="VLI90_18275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HSV16215.1"
                     /translation="MMDTQRIIALVVFSFSALLLWEGWQKHNAPRVPAAASAPAATGI
                     PSHSALNAPGTSTPATPVPGAQPGTAAPPVPVTGAPAVAGQPIEVKTDLFDIEINTAG
                     GDIRRVTMRQVFSAKDLHQPLTLLEANPKHYFVTQSGLLGDALPNHKTPYQADGRSFA
                     LGAGQDTLEVRLTAREGAAEVVKRFTFRRGSYEIKVSYEITNASDKPIAPYAYFQFLR
                     DANPPSEDTVQTSSFAGVTTFTGPAVYTEESKFVKVDFKDIDKGKTPYPKKTKDGWIA
                     MVQHYFVSAWIPKQGVEREYFTNKVGDLYASGVVIPVGTIAPAASTSVEVPVYIGPQ"
     gene            complement(1009..1218)
                     /gene="yidD"
                     /locus_tag="VLI90_18280"
     CDS             complement(1009..1218)
                     /gene="yidD"
                     /locus_tag="VLI90_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HSV16216.1"
                     /translation="MKHVLLWLIAAYRYVLSPWWGGQCRFTPTCSEFASEAIDQYGAI
                     KGSWLASCRVGRCHPWHAGGFDPVP"
     gene            complement(1215..1580)
                     /gene="rnpA"
                     /locus_tag="VLI90_18285"
     CDS             complement(1215..1580)
                     /gene="rnpA"
                     /locus_tag="VLI90_18285"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HSV16217.1"
                     /translation="MPRVARAEGFSRCHRFTAQGSFGAVLRSSRKFRSPHAVLHVSTR
                     LSGCSRLGVALTRRQLPSSVQRNRVKRALREAFRRHPVKSAGLDLVVMFRARIEPSEE
                     RAVVDEVRGLLDQARALRL"
     gene            complement(1586..1720)
                     /gene="rpmH"
                     /locus_tag="VLI90_18290"
     CDS             complement(1586..1720)
                     /gene="rpmH"
                     /locus_tag="VLI90_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HSV16218.1"
                     /translation="MKRTFQPSVAHRKKTHGFRVRMKTAAGRAVINARRAKGRKRVAV
                     "
     gene            1903..>2110
                     /locus_tag="VLI90_18295"
     CDS             1903..>2110
                     /locus_tag="VLI90_18295"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HSV16219.1"
                     /translation="MDSLWPSLAAELKKELPAQQFETWIKPLKAEQRGDLLVLLAPNH
                     HVLRWIRLNVLDRIESRAERVAGGP"
BASE COUNT          396 a          661 c          661 g          392 t
ORIGIN      
        1 tcctgggggc cgatatagac cggcacctct acggaggtgg aggctgcggg agcgatggtt
       61 cccacgggaa tcacgacgcc acttgcgtag agatcgccca ccttgttggt gaagtactca
      121 cgctctacgc cttgcttcgg aatccacgcc gaaacgaagt agtgctgcac catcgcgatc
      181 cagccatcct tcgttttctt cggatagggc gtctttcctt tgtcgatgtc cttgaagtcg
      241 accttcacga acttcgattc ctcggtgtag acggccggcc ccgtgaacgt ggtcacgccg
      301 gcaaaggagc tcgtctgcac ggtgtcctcg ctgggaggat tggcgtcgcg aagaaattgg
      361 aaatacgcgt aaggcgcgat cggcttgtcg gacgcgttgg tgatttcgta gctcaccttg
      421 atctcgtacg atccgcgccg gaaggtgaag cgcttgacga cttccgccgc gccctctcgc
      481 gccgtaagtc gcacctcgag ggtgtcctgc cccgcgccca gcgcgaatga ccgcccgtcg
      541 gcctggtagg gcgtcttgtg gttcggcagc gcgtccccga gcagcccgga ttgcgtaacg
      601 aagtaatgct ttggattcgc ctcgagcagc gtgagcggct ggtgaagatc cttcgcggaa
      661 aagacctgcc tcatcgtcac gcggcggatg tccccgcccg cggtgttgat ctcgatgtcg
      721 aaaagatcgg tcttgacctc gatcggctgg ccggcaacgg caggcgctcc agtgaccgga
      781 acaggtggtg ctgcagtccc gggctgcgcg cccggcacgg gggtggcggg cgtcgacgtg
      841 cccggtgcat tcaacgcgct gtgtgacgga attcccgtag cggcgggcgc gctcgcagcg
      901 gcgggaaccc gtggcgcatt gtgcttctgc caaccctccc agagcaggag tgcggagaag
      961 gaaaagacga cgagggcgat tattcgctgg gtatccatca tgggaatgtc aaggcaccgg
     1021 gtcgaagccg cccgcgtgcc acgggtggca acggccgaca cgacaagagg cgagccagga
     1081 gcctttgatc gcaccgtatt ggtcgatcgc ctctgacgcg aattcggaac aggtcggcgt
     1141 gaagcggcat tggccgcccc accacgggct cagtacatat cgatacgcgg cgatcagcca
     1201 aagcaggacg tgcttcataa gcgcaatgcc ctcgcctgat cgaggagtcc cctcacttca
     1261 tccaccacgg cgcgctcttc cgacggctcg atgcgggcgc ggaacatgac caccagatcc
     1321 aagccagcgc tctttacggg atggcgccga aacgcctcgc gcagcgcgcg cttcacgcgg
     1381 tttcgctgca cggacgacgg cagctggcga cgcgtgagcg caacacccaa tcgagagcat
     1441 ccggacaaac gggttgacac atgaagaacc gcatgggggc ttctgaactt gcgcgaactc
     1501 cgcaggacgg cgccgaacga accttgcgcc gtgaaccggt ggcagcgcga gaagccctcg
     1561 gcccgggcaa ctcgcggcat gaaccctaga ccgcgacgcg cttgcgaccc ttcgcgcgac
     1621 gcgcgttgat cacggcgcgt ccggcggccg tcttcattcg cacgcggaaa ccgtgcgttt
     1681 tcttgcgatg ggcgactgac ggctggaacg tgcgtttcat ggaaaaaacc tctttggttc
     1741 ggcaaggcga aaagcggaaa attatagcgg attcgcgggg aaaacgccat ttctcgcatg
     1801 ctttccggac cttgcgggcc tgtggataac tggcgccatt ccaagtaaaa tttcgattcg
     1861 atttccaccc ccgctcgttt cccgcacacc tccttgctgc tcatggattc cctctggccg
     1921 tcgctcgctg ccgaactcaa gaaagagctg ccggcccagc agttcgaaac ctggatcaaa
     1981 cccctgaagg ccgagcaacg gggtgacctt ttggtcctgc tcgcgcccaa tcaccacgtc
     2041 cttcgatgga tccggctgaa cgtgctcgac cgcatcgagt cgcgggcgga acgagtcgcc
     2101 ggtggaccgc
//
LOCUS       DATIMH010001055         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4000842, whole genome shotgun sequence.
ACCESSION   DATIMH010001055 DATIMH010000000
VERSION     DATIMH010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4000842"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VLI90_18300"
     CDS             <1..290
                     /locus_tag="VLI90_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16220.1"
                     /translation="ARLDDAGGLFISLLADPTTGGVTASFAMLGDFIIAEPKALIGFA
                     GPRTIWNTIKVELPEGFQRAEFLEEHGFVDFVVHRKDLRSEIARLVDFCVK"
     gene            1360..2439
                     /locus_tag="VLI90_18305"
     CDS             1360..2439
                     /locus_tag="VLI90_18305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HSV16221.1"
                     /translation="MDDIAPTRHAESWDNVGLLVGDPAQSIIRVMLTIDYTAAVAAEA
                     RNENCDCIIAYHPPIFDAIKRITADGPSGLIHDAIRRGVAIYSPHTALDVADGGTNDM
                     LADAIGLPAENRQPLRLAQTKAMQYKLVVFVPERELDAVSSAMFAAGAGRIGKYSSCS
                     FRSPGTGTFFGEAGTNPAVGESGKLETAQEVRLETVVPISKIHSVIEAMRSAHPYEEP
                     AFDLNQLAAPPEGVGQGRIASFASPVLISELIGRIKRELEIESVLTAGPTEGSVSRAA
                     VCAGSCGEFLDDAISQNAEFFLTGELRHHDAIKAANAGMTVICTLHSNSERAVLKRLR
                     SRLQQRLPSVEFLLSQADRDPFVIR"
     gene            complement(2348..>2935)
                     /locus_tag="VLI90_18310"
     CDS             complement(2348..>2935)
                     /locus_tag="VLI90_18310"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HSV16222.1"
                     /translation="AWWGVREELVECRDAVDVLLAESLHLQLDAFIERAGADGELAGD
                     PHALRIAGKSLRYTLEMAIEEGHRLPATVMRAFKQMQDALGLWHDFVVLAERLMDVSL
                     DEQLPHHDADMQAGVLELTVLCVRSSARQLDRFSSLWSARGEELTRTIRQTFPLSRPS
                     PTASIVNESRMDRDQPDSAEIPPTATAAEGETSAA"
BASE COUNT          572 a          902 c          907 g          554 t
ORIGIN      
        1 tggcgcggct cgacgacgcc ggcggcctct ttatatcatt gctagcagat ccaaccaccg
       61 gcggcgtcac cgccagcttc gccatgctcg gcgatttcat catcgccgag cccaaagcgc
      121 taataggttt cgccggcccg cggacgattt ggaatacgat caaggtcgag ctgcccgaag
      181 gttttcagcg cgcggagttt ctcgaagagc acgggttcgt cgatttcgtc gtgcatcgca
      241 aggacctgcg cagcgagatc gcccggctcg tggatttctg cgtgaagtga tctccatatg
      301 ggcccgcata cagaggaact ttttctgacc cttgactgtg ctcgagcccg acaagcggcc
      361 gaacggtttg ggcagaaaat ccaagctcgt tttcgaacgt ggtcgtttcg gccggaagta
      421 ctctcgatag acctcacgct cattaaaccg agtccggcgc ttgtgccaga gcttccggaa
      481 atcgacgtag caccttacgt aagatttttc cgcgacaatc tagagccaga cgggactccc
      541 gacgacaacg agctccttgt aaggattcaa tgcctgcatt ggttcactcg caccaccggc
      601 cagccggctg caatatggac tcaccttggt tggaagggct tgttacacga gtgctgtctt
      661 ggcgtggggg gcgcggaacc cattctgcac ttcaatgagg acgaacggcg atccagtttg
      721 acggcggcgc tgtggtacct tggcgttgac gtgccggatc attcggaaag ctactggatt
      781 catgatggag tttatgcggc gcagattgca agccgccgca aagcggcaat cgagaacggg
      841 acaaaccccg aatcaaggtt tggctcgcga tggtgattcg ccagcctgat caagtccaga
      901 gaactaattc cggcgaggcc agaaatgtta tgaggactgc gctaatgacg atcgacatag
      961 cgacgcgagt ttggcgcctc ggaagcatgt acatcgcccg cgctctgcca ctcgatgtct
     1021 ccagtgccgg cgcgacaccg gacgcagctc gcgcagcgct tcgagaggcg attgcactct
     1081 tcgtcgccac ggcgcgggat ttgggaacaa ttgaggaggt tctcgaagag tgcgggtaca
     1141 cgcaggcgca tgaacgttgg cacgcgcctg caatcgagcc cggagtgcgt gaatcacttg
     1201 tggtgtaaca ctcgtgtcca gcgccggacc acgtcgcgcg cggagcgtac tcgcgaggag
     1261 ccggatctgc cgccgcgaga gaattcccag tcgtcgcaac tcgctccgaa cccggcgtgt
     1321 gcacgtgtca aaatgcaatt gcgcgaccta atcagcggct tggatgatat tgcccccacg
     1381 cggcacgcgg aatcgtggga caacgtcggt ctgctcgtcg gcgatcctgc gcagtcgatc
     1441 attcgcgtga tgctcacaat cgactacacc gccgcggtgg cggccgaggc gcgcaacgag
     1501 aattgcgact gcatcattgc atatcacccc ccgatcttcg acgcgatcaa gcgcatcacc
     1561 gccgacggtc cgtccggttt gatccacgac gccattcgtc gcggcgtggc gatttattcg
     1621 ccgcacacgg cgctcgatgt cgcggacggc gggacgaatg acatgctcgc cgacgcgatc
     1681 ggtttgccgg cggagaatcg gcagccgttg cggttagcgc agacgaaggc gatgcagtac
     1741 aagctcgtcg tcttcgtacc cgagcgcgaa ctggacgcgg tcagcagcgc gatgttcgcc
     1801 gcgggggcgg ggcggattgg caagtattct tcgtgcagct tccgctcgcc cggcaccggc
     1861 acgttcttcg gcgaagccgg caccaacccg gccgtgggtg aaagcgggaa gctggaaacc
     1921 gcgcaggaag ttcggctaga aacggttgtc cccatctcca aaatccacag cgttatcgaa
     1981 gcgatgcgat ccgcccatcc ctacgaagaa cccgcgttcg atttgaatca gttggcggcg
     2041 ccgccggaag gcgtggggca ggggcgcatt gcctcattcg cctcgccggt actgatttcg
     2101 gagttgatcg ggcgaatcaa gcgcgaactg gaaatcgaat cggtgctcac cgccgggccc
     2161 accgagggga gcgtgagtcg agccgccgtc tgcgccggct cgtgcgggga atttctggac
     2221 gatgcgatct cgcaaaacgc ggagtttttt ctaacggggg agttgcggca tcacgacgcc
     2281 atcaaggccg ccaacgccgg catgaccgtc atctgcacgc tgcattccaa cagcgaacgg
     2341 gcggtgctca agcggctgag gtctcgcctt cagcagcggt tgccgtcggt ggaatttctg
     2401 ctgagtcagg ctgatcgcga tccattcgtg attcgttgac gatcgaagct gtcggcgacg
     2461 gccggctgag cgggaacgtc tggcggatcg tccgcgtcaa ctcttcgccg cgggcggacc
     2521 agagtgatga gaaccgatcc agctgacgcg ctgagctgcg gacgcacagc accgtcagtt
     2581 cgagcacgcc cgcctgcatg tccgcatcgt gatgcggcag ctgctcgtcc agcgacacgt
     2641 ccatcaaccg ctcggccagc acgacgaaat catgccacag gcccagcgca tcctgcatct
     2701 gcttgaacgc ccgcatcacc gtggccggca ggcggtggcc ttcctcgatc gccatctcca
     2761 gcgtgtatcg cagcgacttt ccggcgatgc gcagcgcgtg cggatcgccg gccagctcgc
     2821 cgtcggcgcc ggcgcgctcg atgaacgcgt ccagctgaag atgcaacgat tcggccagca
     2881 gcacatcgac ggcatcgcga cattcgacca attcttcccg cacgccccac caggc
//
LOCUS       DATIMH010001056         1985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4295550, whole genome shotgun sequence.
ACCESSION   DATIMH010001056 DATIMH010000000
VERSION     DATIMH010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4295550"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..>1985
                     /locus_tag="VLI90_18315"
     CDS             28..>1985
                     /locus_tag="VLI90_18315"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSV16223.1"
                     /translation="MAQDKLWELANYELKPRLNRRSGVSSIIVQGGKVPEFEVQPDPA
                     KLVQAGVTIPNILDAINRSNMIDSPGLIEANHQLVLSLVSGQARTPKEIGDMVIKTTP
                     AGAPLRIGDVASVGPSVMPVYTAVTANAKPAVLLNVYRQPDSNTVVVADGIKDEIAAI
                     RKDLPKGIDLRPFYDQSEIVKDSIDSVRDAILIGLVLASLIMVLFLRDWGTSLVAGLV
                     IPATIAVTFIMLRALNQTFNLMTLGGLAAAVGLVIDDAIVVVENIVMHRDLGQTRAEA
                     IRNAIREIRAPLVGSTITPIVVFVPLISITGVTGVFFRALAITVGMSLLTSLALALTW
                     TPTLSHYFIRKQKHHAAKAEQGRLMRIYERTLRTTLEHPLILAVFAALLIGGSYFCYK
                     HTGSDLMPAMDEGGFIVDYIMPAGSSLEETNRVITHVEEILRKTPEVESTSRRTGLQL
                     GLAQVTEANTGDISVKLKRDRKRSGEDVIAAVRAKIKKAEPVLDVEFPQLLQDMIGDL
                     TSAPEPVVIKMFAQDPDLLRAWAPQVAGAIKKIPGVVDVLDGIENTISGPATVFTVDA
                     AAAARAGFTTQEVELDASAMLQGEPAPPPVVVNDRAYGIRVRFPLATRKSLDTIQNTL
                     LISGAGKTGTIGSLASMKEMPGQTEIRR"
BASE COUNT          400 a          586 c          657 g          342 t
ORIGIN      
        1 atgggctaca gcctgacttc ggaaactgtg gctcaggaca aactgtggga actagcgaac
       61 tacgagctga agccgcggtt gaatcggcga agcggggtgt cctcaattat cgtgcagggc
      121 ggcaaagtgc cggagttcga agtgcagccc gatccggcca aactggtgca ggccggcgtg
      181 acgatcccga atatcctcga tgccatcaac cgcagcaaca tgatcgattc gccgggcctg
      241 atcgaggcca atcaccaact ggtgctcagc ctcgtcagcg ggcaggcgcg cacgccgaag
      301 gaaatcggcg acatggtgat caaaacgacg cccgcgggcg cgcccctgcg catcggtgac
      361 gtagcaagcg tcgggccgag cgtgatgcct gtctacaccg cggtgacggc aaacgccaag
      421 cccgccgttc tgctcaacgt gtaccggcag cccgacagca acaccgtcgt agtcgcggac
      481 ggaattaagg acgagatcgc cgccatacgc aaggacctgc ccaagggcat cgaccttcgg
      541 ccgttctacg accaatcgga aatcgtcaag gattccatcg atagcgttcg cgatgcgatc
      601 ctcatcgggc tggtgctggc gtcgctgatc atggtgctgt tcctacgcga ttggggtacg
      661 tcgctggtgg cagggctggt gatccccgcg actatcgcgg tgacgttcat catgctgcgc
      721 gccctgaacc agacgttcaa cctgatgacg ctgggcggac tggcggcggc cgtgggcctc
      781 gtgatcgacg acgccatcgt ggtggtggaa aacatcgtga tgcaccgcga cctgggccaa
      841 acgcgcgcgg aagcgattcg caatgcgatt cgcgagatcc gcgcgcccct ggtggggtcg
      901 acgattacgc cgattgtggt cttcgtgccg ctgatttcga ttactggagt aaccggcgta
      961 ttctttcgtg cgctcgcgat cacggtaggc atgagcctgc taacgtcgct cgccctggcg
     1021 ctgacatgga ctccgactct gagtcattac ttcatccgca agcagaagca tcacgccgcg
     1081 aaggcggaac agggtcgcct gatgcggatc tacgaacgca cgctgcgcac aacgctggaa
     1141 cacccgctca tattggcggt gttcgcggcg ctgctgatcg gcggcagtta tttctgctac
     1201 aagcacacgg gcagcgattt gatgccggcc atggacgaag gcggtttcat cgtggactac
     1261 atcatgcccg cgggatcgtc gctcgaagag accaatcgcg tgatcacgca cgtcgaagag
     1321 attctgcgca agactcccga ggtggagagc acatcgcggc ggaccggcct gcaattgggg
     1381 ctggcgcagg tgacagaggc caacaccggc gacatttcgg tgaagctgaa gcgcgaccga
     1441 aagcgcagcg gcgaggacgt gatcgcggcc gtgcgcgcca agattaaaaa ggcagagccc
     1501 gtgctcgacg tggaattccc gcagctcttg caggacatga taggcgatct gaccagcgcg
     1561 cccgaacctg tcgtaatcaa gatgttcgcg caggacccgg atttgctgcg cgcgtgggcg
     1621 ccgcaagtgg cgggcgcgat caagaaaatt cctggcgtgg tggacgtgct cgatggcatt
     1681 gagaacacca tcagcggtcc ggcgacggta ttcacagtgg atgccgcggc ggccgcgcgg
     1741 gcgggcttca ccacgcagga ggttgagttg gacgcgagcg cgatgttgca aggcgagccg
     1801 gcgccgcccc cggtggtggt gaacgatcgc gcgtatggaa tccgcgtgcg ctttccgctg
     1861 gccacgcgca agtcgctgga cacgatccag aatacgctgc tgatctcggg cgcgggaaag
     1921 acgggcacca tcgggtcgct cgcgagcatg aaggagatgc ccgggcagac cgagatccgg
     1981 cgcga
//
LOCUS       DATIMH010001057         6463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5455507, whole genome shotgun sequence.
ACCESSION   DATIMH010001057 DATIMH010000000
VERSION     DATIMH010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5455507"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VLI90_18320"
     CDS             <1..1023
                     /locus_tag="VLI90_18320"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HSV16224.1"
                     /translation="DAGGYGMDALWNDDFHHSAMVALSGHNEAYYSDYLGKAQELLAA
                     AKWGYLYQGQRYRWQRKRRGTPALDVPPTAFVHFLQNHDQVANSGRGYRAHQITSPGE
                     LKAMTALLLLMPQTPMLFQGQEFAASNPFYYFADHNPELAKRVCAGRAKELAQFPSVA
                     TPQMQACLLDPADRATFERSKIDHTERTCANHALIWQLHKDLLQLRREQPVFRRVQRR
                     GDIDGAVLGDGAFVLRYFGGSDRLLVVNLGRDLELNPCPEPLMATPLDMRWSILWSSE
                     DPKYGGSGTAALDTENEGWLIPGRSAVVLEPLPAEQAAVQTRVRANGSAQAAIPKEAL
                     SGTRSG"
     gene            1114..3714
                     /gene="treY"
                     /locus_tag="VLI90_18325"
     CDS             1114..3714
                     /gene="treY"
                     /locus_tag="VLI90_18325"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HSV16225.1"
                     /translation="MSPQALYPESTYRLQFHKGFTFGDAIAIVPYLAELGITHVYASP
                     YLKARPGSTHGYDVIDPCSINPEVGNEEDYRAFLDALRANGLGHILDIVPNHMGVATN
                     ENQWWNDVLAHGRTSRYAGFFDIAWRGSPRPAMHDKVLLPVLGEPYGEALEAGKLRLS
                     VEGDRYFANYYDHRFPIAPETRNAAAEAMEKGGADRLDALLNRQHYRLAYWRVASDEI
                     NYRRFFDINDLAALCMEREEVFDATHALVFRLVAEGRVAGLRIDHPDGLYDPQQYLQR
                     LRQRQPGLYVVVEKILAVDEELPTSWPVNGTTGYDFLNKVNGLFIDPAGREPLTRLYH
                     EWTGGDTTFPELAYEKKRLILNTSLASELQMLAHQLDQIAQSDRRSRDFTLRALRDGL
                     REVIACFGVYRTYITDRQVTETDQRYIRSAIEQATERNPAISTAVFGFIEQSLLTKPA
                     FAGRFQQVTAPVTAKGIEDTAFYIYNRLVSLNEVGGDPDHFGVSTDALHAYLSDRQRH
                     WPHALSPLSTHDTKRSEDVRARINVLSEIPDEWRTHVQRWREMNRGFKSELARPTPGL
                     RYAEDPDQLRQDPGLRRTSDPASGDHSAPSQNDEYLLYQTLLGAWPIGADRLKAYMTK
                     ALREAKLHSSWTSPNEAYEAATHRFINSILASRPFLDDFQTLHRRVSHLGYLNSLSQT
                     LLRLAAPGAADTYQGAELWDFSLVDPDNRRPVDYVRREQMLGQLRASPQELLRDLDDG
                     RVKLWMTQQTLRCRRDHPGLFSRGEYLPLEVMGACRENIFAFCRRQGDVSAIVIVPRL
                     MAKFVTGNSLPIGRGVWADTKLTINGETPTGLRDLFTGRWHDGRNGIDIADVLTDLPV
                     SILLRASSAL"
     gene            complement(3772..4545)
                     /locus_tag="VLI90_18330"
     CDS             complement(3772..4545)
                     /locus_tag="VLI90_18330"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HSV16226.1"
                     /translation="MTTVDSNPLHARSDRILIVTSLVILTAAAWAYLIHDVTSGVNNL
                     PACCLRADPRFWGWRDFTVLFVMWTVMMIGMMTPTVAPMVLMFAAINRRRREQLRPFV
                     PTALFLLGYLIIWTAFSGVATGAQYGLHRASLISPAMVATNRWLGAAVLIAAGIFQFT
                     PAKRRCLTHCRSPLDFIMTDWRDGRRGAIVMGLRHGIACTGCCWLLMALLFVVGVMNL
                     LWVAAITLFVLAEKALPVGPWLPRISGAALIVWGVASLL"
     gene            complement(4619..5242)
                     /locus_tag="VLI90_18335"
     CDS             complement(4619..5242)
                     /locus_tag="VLI90_18335"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HSV16227.1"
                     /translation="MANTPATKWFIEADYIQACNCDYGCPCEFEAPPTMGFCQGMGAW
                     RITKGKYGDVALDGCAFAGALKTPAAMHLGNGTLVIFIDERATPPQREALQMIGSGRA
                     GGMPFEAFQMIFSKWMGPIFAPITFNFDAGNSSVNIGDAMNVQMEPIKNPVTGEAESI
                     RIEHGTGFIFKSADCYSAKENRVNAPGMMEFSYPNKSGFVTKVRYGN"
     gene            5384..>6463
                     /gene="amrS"
                     /locus_tag="VLI90_18340"
     CDS             5384..>6463
                     /gene="amrS"
                     /locus_tag="VLI90_18340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HSV16228.1"
                     /translation="MLHQYSVPAAEALIEHRPDGAVQCFACGHRCIVKPGRDGVCRVR
                     FNEDGTLLVPHGYVGALACDPIEKKPFFHVLPGSDALTFGMLGCDYHCGYCQNWVTSQ
                     MLRDPDAVAPPRMTTPQQLVDIAIQNRAPVVASSYNEPLITSEWAVDVFKEARSRGLK
                     CAYISNGNGTPQVLDYIRPYVDAYKVDLKTFDDKHYRELGGVMENVTSTIKMLKERGF
                     WVEIVTLVVPGFSDDPDDLKRMADFIASVDPLMPWHMTAFHPDYKMTDGYRSTTVDDL
                     MRIVEFGKQAGLQYMYPGNLPGQVGEWENTRCHHCRTTVIKRFGFRVLKSLVNADGSC
                     PTCHKQIPGIWGQSSGHGDGRVRPLS"
BASE COUNT         1256 a         2039 c         2058 g         1110 t
ORIGIN      
        1 gacgccggtg gatacgggat ggacgcgctg tggaacgacg atttccatca cagcgcgatg
       61 gtggcattgt cgggtcataa cgaggcgtac tacagcgatt atctgggcaa agcgcaggag
      121 ctgctggcgg cggcgaagtg ggggtatctg tatcaagggc agcgctaccg gtggcagcgc
      181 aaacgtcgcg ggacgccggc gctggacgtg ccgccgacgg cgttcgtgca cttccttcag
      241 aaccatgacc aggtggcgaa ttccggccgc gggtatcgcg cgcatcaaat caccagtccg
      301 ggagaactga aggcgatgac ggcgctgctg ctgctgatgc cgcaaacgcc gatgttgttc
      361 cagggacagg aattcgccgc gtcgaatccg ttttattatt tcgccgatca caatccggag
      421 ctggcgaagc gggtgtgcgc cggccgcgcg aaggagttgg cgcagtttcc cagcgtcgcc
      481 acaccgcaga tgcaggcgtg cctgctggac ccggccgatc gcgcgacgtt cgagcgatcg
      541 aagatcgacc acaccgagcg cacgtgcgcg aaccacgcgc tgatctggca actgcacaag
      601 gacctgctgc agctgcgccg cgagcagccc gtcttccgcc gcgtgcagcg gcggggggat
      661 atcgatggcg cggtgctggg cgacggcgcg ttcgtgctgc gctacttcgg cggcagcgat
      721 cggttgctgg tggtgaatct cggcagggat ctggagctga atccatgccc cgagccgctg
      781 atggcgacgc cgctggatat gcgctggtcg atcctctggt ccagcgaaga ccccaaatac
      841 ggcggcagcg gcaccgcggc gcttgacacc gagaacgaag gctggttgat ccccggtcgc
      901 agcgccgtcg tgctggaacc gctgccggcc gagcaggcgg cggtgcagac gcgcgtgcgc
      961 gccaatggct cggcgcaagc ggccataccg aaggaagcat taagcggaac acgctccggg
     1021 tgacacgggc ctccggcccg tgcgaggtgc tcatgaatga cggaaaacac cagctccact
     1081 aactcctacg ttgcacgcac gggccagacg cccgtgtcac cgcaagcgct ctatcccgaa
     1141 tcaacctatc gcctgcagtt ccacaagggc ttcacgtttg gcgatgccat cgcaatcgtg
     1201 ccgtacctgg cggagctggg catcacgcac gtgtacgcgt cgccgtacct gaaggcccgg
     1261 cccggcagca cgcacgggta tgacgtgatc gacccgtgct cgatcaatcc agaagttggc
     1321 aacgaagagg attaccgcgc gtttctggac gcgctgcgcg cgaatggctt aggccacatc
     1381 ctcgacatcg tccccaacca catgggcgtc gccaccaacg aaaaccagtg gtggaacgac
     1441 gtgctggccc acggccgaac gtcgcgctac gcgggattct tcgacatcgc ctggcgcggc
     1501 tccccgcggc cggcgatgca cgacaaggtg ctgctgccgg tgctgggcga accgtacggc
     1561 gaagcgctgg aagccggcaa gctgcggttg tcggtcgagg gggatcgcta cttcgccaac
     1621 tattacgacc accgatttcc catcgcgccc gagacgcgca acgcggccgc tgaggcgatg
     1681 gagaagggcg gagcggatcg gctggatgcg ctgctgaacc gccagcacta tcgcctggcg
     1741 tactggcggg tcgcgtcgga tgagatcaac taccgccggt tcttcgacat caacgacctg
     1801 gccgcgttgt gcatggaacg cgaggaagtg ttcgacgcga cgcatgcgct ggtgtttcgg
     1861 ttggtcgccg agggaagagt cgcggggctt cgcatagatc accccgacgg gttgtacgat
     1921 ccacaacaat atctgcagcg gcttcgccag cgccagcctg gactgtacgt cgtcgtcgaa
     1981 aaaatcctcg cggtcgatga agagctgccc acgtcctggc cggtcaatgg cacgaccggg
     2041 tacgacttcc tgaacaaggt caacggcctg ttcatcgacc ccgccgggcg cgagccgctc
     2101 acgcggctgt atcacgagtg gaccggcggc gacacgacct ttccagagct ggcgtacgag
     2161 aagaagcggc tgatcctcaa cacctcgctc gccagcgaac tgcagatgct cgcgcatcag
     2221 ctggatcaga ttgcgcaatc cgaccgccgg tcgcgcgact tcacactgcg cgcgctgcgc
     2281 gacggcctgc gcgaggtgat cgcgtgcttc ggcgtctatc gcacgtacat caccgatcgc
     2341 caggtgacgg aaacggacca gcggtacatc cgctcggcga tcgagcaggc gaccgagcgc
     2401 aatcccgcga taagcaccgc ggtgtttggg ttcatcgaac aatcgctgct gacgaagccc
     2461 gccttcgccg gacgattcca gcaggtgacc gcgccggtga ccgccaaggg catcgaagac
     2521 accgcgttct acatctacaa ccggctggtc tcgctgaacg aagtgggcgg tgaccccgat
     2581 cacttcggcg tgtcaaccga cgcgctgcat gcgtatttgt cggatcgaca gcggcactgg
     2641 ccgcacgcgc tgtcgccgct atcgacgcac gacacgaagc gaagcgaaga cgtgcgggcg
     2701 cggatcaacg tgctgtcgga gattccggat gaatggcgca cgcacgtgca gcgctggcgc
     2761 gagatgaacc gagggttcaa gagtgagctg gcgcgaccga cgccgggcct gaggtacgcc
     2821 gaagacccgg atcagttgcg ccaagacccg ggtcttcggc gtacctcaga cccggcgtcg
     2881 ggggaccatt cggcgccgtc ccagaacgac gagtatctcc tttatcaaac gctgctcggc
     2941 gcgtggccca tcggcgcgga tcggctcaag gcgtacatga ccaaggccct gcgcgaagcg
     3001 aagctgcaca gcagctggac ttcgccgaat gaggcgtatg aagccgcgac gcaccggttc
     3061 atcaactcga tcctcgcgtc gcggccgttt ctggatgact tccaaacgct gcaccgccgc
     3121 gtgagccacc ttgggtatct caactccctc agtcaaacgc tgctgcggct ggcggctccc
     3181 ggcgcggccg acacctatca aggcgccgaa ctgtgggact tcagcctcgt tgatcccgat
     3241 aatcgtcggc cggtcgatta cgtccgtcgc gagcagatgc tgggacaact tcgcgcatcg
     3301 ccgcaggagt tgctgcgcga cctggatgat ggccgggtaa agctgtggat gacgcagcaa
     3361 acgctacgtt gccggcgcga tcatccgggc ttgttttccc gcggtgaata ccttccgctg
     3421 gaagtgatgg gcgcctgccg cgaaaacatc ttcgcattct gccggcggca aggggatgtg
     3481 tctgcgatcg tgattgtccc gcgactgatg gcgaagtttg tgactgggaa ctcactcccc
     3541 atcggacgcg gcgtctgggc ggatacaaaa ctcaccatca acggcgagac tccgaccggc
     3601 ctccgcgatc tgttcaccgg ccgatggcac gatggccgga atggcattga tatcgcggac
     3661 gttctaaccg acctgcccgt ttcgattctg cttcgggctt cgtccgcctt gtagccgcga
     3721 cgcctgcgtc gccttgcttc tccacgcgat gaggcgacgc aggcgtcgca gctacagcaa
     3781 gctcgcaaca ccccagacga tgagcgcggc gccgctgatc cgcggcagcc agggtcccac
     3841 cggaagcgcc ttttcggcca gcacgaacag cgtgatcgcc gcgacccaca gcaggttcat
     3901 tacgccgacg acgaacagca gcgccatcag caaccagcag cagccggtgc acgcgatgcc
     3961 gtggcgcagg cccatgacga ttgccccgcg gcggccatcg cgccaatcgg tcatgatgaa
     4021 atccagcggc gaacgacagt gcgtcagaca ccggcgtttg gccggcgtga attgaaagat
     4081 tcccgcggcg atcagcaccg cggcgccgag ccagcggttg gtcgcgacca tcgcgggcga
     4141 gatcagcgag gcgcgatgca ggccatactg cgcgccggtc gcgacgccgc tgaaggcggt
     4201 ccagatgatg aggtagccga ggagaaacag cgcggtcggc acgaacggcc gcagctgctc
     4261 gcgccgccgg cggttgatgg cggcgaacat cagcaccatc ggcgcgacgg tcggcgtcat
     4321 catgccgatc atcatcacgg tccacatgac gaacaggaca gtgaagtcgc gccaccccca
     4381 aaaacgcgga tccgcccgca ggcagcacgc gggaaggttg ttcacgccgc tggtgacgtc
     4441 gtggatcagg tacgcccacg cggcggcggt gaggatcacc agcgatgtga cgatgaggat
     4501 ccgatcgctg cgcgcatgaa gcggattgga gtcaacggtt gtcatgaagc ttgccgcttg
     4561 cggctttgca aaccgggcaa cctggtctgc gaagccgcaa gcggcggggg agaagaaatc
     4621 aattcccgta gcgcaccttc gtcacgaagc ccgatttgtt gggataggag aactccatca
     4681 tccccggcgc gttcacgcgg ttttctttgg cggagtaaca gtcagcgctt ttgaagatga
     4741 acccggtgcc atgttcgatg cggatcgatt cggcttcgcc cgtcacggga tttttgattg
     4801 gctccatctg cacgttcatc gcgtcgccga tgttcacgct gctgttgccg gcgtcgaagt
     4861 tgaaggtgat cggcgcgaag atcggtccca tccatttgga gaaaatcatc tgaaacgcct
     4921 cgaacggcat cccaccggct cggccgctgc cgatcatctg cagcgcctcg cgctgcggcg
     4981 gcgtggcgcg ctcatcaatg aagatgacca gcgtgccgtt gcccaggtgc atggcggccg
     5041 gcgtcttgag cgcgccggcg aaggcgcagc cgtccagcgc gacgtcgccg tactttccct
     5101 tggtgatgcg ccacgcgccc atgccctggc agaagcccat tgtcggcggg gcttcgaact
     5161 cgcagggaca gccgtaatcg cagttgcagg cctgaatgta gtcggcctcg atgaaccact
     5221 tcgttgcagg tgtgttcgcc atgattgcgc tcctttagcc aggtgaccta aaaaatattc
     5281 cgcaggaaaa cgcgcagaat tgcaactaag attttccgtc atgccaggtg gtatttcact
     5341 ggaaatcgtt gagcaaaaag agcgattaaa atcgcttaag cagatgctgc atcaatacag
     5401 cgtccccgcg gccgaggcgc tgatcgaaca caggccggac ggtgccgttc aatgcttcgc
     5461 ctgcggccac cgctgcatcg tcaaacccgg tcgcgacggt gtctgccggg tgcgcttcaa
     5521 cgaagacggc acgctgctcg tcccgcatgg gtatgtcggg gcgctggcat gcgacccgat
     5581 cgaaaagaag ccgttcttcc acgtcctgcc cggcagcgat gcgctgacct tcggcatgct
     5641 cggctgcgat tatcactgcg gctactgcca gaattgggta accagccaaa tgctgcgcga
     5701 ccccgacgcc gtggccccgc cgcggatgac gacgccgcag cagctggtgg atatcgcgat
     5761 tcaaaatcgc gcgccggtcg tcgcgtcgag ctacaacgag ccgctcatta ccagcgaatg
     5821 ggccgtcgat gtgttcaagg aagcccgatc gcgcggcctc aagtgcgcgt acatcagcaa
     5881 cggcaacggc acgccgcagg tgctcgatta catccgcccg tacgtcgacg cgtacaaggt
     5941 cgatctgaaa acgttcgacg acaaacacta ccgcgaactt ggcggggtga tggagaacgt
     6001 caccagcacg atcaagatgc tcaaagaacg cggcttctgg gtcgagatcg tcacgctcgt
     6061 cgtccccggc ttcagcgatg atcccgatga cttgaaacgc atggccgact ttatcgccag
     6121 cgtcgatccg ctcatgccct ggcacatgac cgcgttccac cccgactaca agatgaccga
     6181 cggctaccgc agcacgacgg tggacgacct gatgcgcatc gtcgaattcg gcaagcaggc
     6241 gggccttcaa tatatgtatc ccggcaacct gcccgggcag gtgggcgagt gggagaacac
     6301 gcgctgccat cattgccgga cgacggtgat caagcgcttc ggcttccgcg tgctcaagtc
     6361 actggtgaac gccgacggca gctgcccgac gtgccacaaa cagatccccg gcatctgggg
     6421 ccagtccagc gggcatggcg atgggcgcgt tcggccttta tct
//
LOCUS       DATIMH010001058         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_250768, whole genome shotgun sequence.
ACCESSION   DATIMH010001058 DATIMH010000000
VERSION     DATIMH010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250768"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /locus_tag="VLI90_18345"
     CDS             complement(<1..2056)
                     /locus_tag="VLI90_18345"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSV16229.1"
                     /translation="MRLKKLIVQGFKSFADRTEFVFDSPITGIVGPNGCGKSNVVDAF
                     KWVLGEQSAKSLRGDAMMDVIFNGAAARKPSGMAEVVLVFDNPKREDDTRMLAMDVDE
                     VAVGRRLYRDGTSEYHLNNGSSRLKDIRELFLDTGVGADAYSVIEQGKVSAMLEANPA
                     ERRVIFEEAAGISKYKVKKKEAQRKLEKVDQNLLRVNDIVEEVDKRLRSVKIQAGRAR
                     VYQEHSTRLGELRLGYALQEYHTHHQALGGLEKEREDLQFRLDDLAGDLAARQNELAQ
                     KREQFDALSQQKQRIEYDLVQARAAVQSAQQRQHYAEQQLQQMAEQLEAFERDKADLE
                     AKLAVVTESLTTETSRLQSLTTELQQHREQIEQRQQAFRDGQLQLNKTNQEIEQNKAA
                     VLDLMRKLASVGSRLGAIEIERKNIAAQQQRLGDRRRIVMDELAGLETKKQELQEKLD
                     AALADIAEQQGLLEAKKQEAAALGKQIAQVGEQLGAAKEHRSGLVSRQRLLKDLEAKR
                     EGVSEGVKSVLRERDTKFPFIRGLVADILRVDVEHAHVIEAALDGRDQWLITNDQSLA
                     IAADTALNDLEGRVNIVSVDRINSSTGVSPVPSDVGHRRDARAAELYDWNQHPQRIRL
                     AIDLVKVDPADAAIAQHLLGRSIIVDDLAAAAELHRVGPAGYRYVTQTGDVIESDGTL
                     RAG"
     gene            complement(2194..>2466)
                     /locus_tag="VLI90_18350"
     CDS             complement(2194..>2466)
                     /locus_tag="VLI90_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16230.1"
                     /translation="VVAIVQGMVEQQKVSGLCGTQRQLAAFVLQGPPAVEATASVDTA
                     PLLRDNGRPQSERPNVPKEQATPTPAAAEQRPAQVGSAELEASNGK"
BASE COUNT          372 a          819 c          727 g          548 t
ORIGIN      
        1 ggccggcgcg gagcgtgcca tcggactcga tgacgtctcc ggtctgggtg acgtagcgat
       61 accccgcggg gccaacgcgg tgcagttccg cggccgcggc gaggtcgtcc acgatgatcg
      121 atcggccgag caggtgctgg gcgatcgccg cgtcggcggg gtccactttg acgaggtcga
      181 tggcaaggcg gatgcgctgc gggtgttggt tccagtcgta caactccgca gcacgggcgt
      241 cccgcctgtg tccaacgtcg ctcggcacgg gcgagacgcc cgtgctactg ttgatccgat
      301 cgacggacac tatgttgacg cgcccttcga gatcgttcag tgctgtatcc gcggcgatgg
      361 cgagcgattg atcattcgtg atgagccact gatcgcgccc atccagtgcc gcttcaatca
      421 cgtgagcatg ttccacatcg acgcgaagga tgtcggcgac gagcccgcgg atgaacggga
      481 acttcgtgtc gcgctcgcga aggaccgact tcacgccttc gctcacgcct tcgcgtttcg
      541 cttcgagatc cttcagcaat cgctggcgag acaccaagcc cgaacgatgt tccttcgccg
      601 ccccgagttg ctcgccaact tgcgcgatct gcttgcccag cgccgcggct tcctgcttct
      661 tcgcttcgag caacccctgc tgctcggcga tgtccgccag cgccgcgtcc agcttctctt
      721 gcagctcctg cttcttcgtc tccagtccag ccagctcgtc catgacgatc cgccggcggt
      781 cgccgaggcg ctgctgctgg gccgcgatgt tcttgcgttc gatctcgatc gcgcccaagc
      841 ggctgccgac gctggcgagc ttgcgcatca ggtccagcac ggcagccttg ttctgctcga
      901 tttcctgatt ggtcttgttc agctgaagct ggccgtcgcg aaacgcctgc tggcgctgct
      961 cgatctgctc gcgatgctgc tgcagttccg tcgtcagcga ttgcagtcgc gacgtttcgg
     1021 tcgtcaagga ttcggtgacc accgccagct tcgcttccaa gtccgccttg tcgcgttcga
     1081 atgcttccag ctgctccgcc atctgctgca gttgctgctc ggcgtagtgt tgccgctgct
     1141 gggccgactg caccgccgcc cgcgcttgca ccaggtcgta ctcgattcgc tgcttctgct
     1201 gcgaaagcgc atcgaattgc tctcgcttct gggccaactc gttctgccgc gccgccaggt
     1261 cgccggccag atcgtccagg cggaactgca ggtcttcgcg ttccttctca aggccgccga
     1321 gggcctgatg gtgcgtgtga tattcctgca gcgcgtagcc cagccgcagt tcgcccagcc
     1381 gcgtggaatg ttcctggtag actcgcgcgc gcccggcctg gatcttcacg ctgcgcagcc
     1441 gcttgtcgac ttcctcgacg atgtcgttca cgcgcagcag gttctgatcg accttttcca
     1501 gcttccgctg ggcttccttc ttcttgacct tgtacttgct gatccccgcg gcctcttcga
     1561 agatcacccg ccgctcggcc gggtttgctt cgagcatcgc cgacactttt ccctgctcga
     1621 tgacgctgta cgcatccgcg cccacgccgg tatcgaggaa tagctcgcgg atatctttca
     1681 ggcgcgacga accgttattg aggtgatatt cgctggtgcc gtcgcgatac agccgccggc
     1741 cgacggccac ttcgtccacg tccatcgcca gcatgcgcgt gtcgtcttca cgcttagggt
     1801 tgtcgaagac gaggactact tccgccatgc ccgacggttt gcgggcggct gcaccgttga
     1861 agatcacgtc catcatcgcg tcgccgcgca gcgacttagc cgactgctcg ccgagcaccc
     1921 atttgaaggc gtcgacgacg ttggacttgc cgcaaccgtt gggaccgacg atgccggtga
     1981 tgggggaatc gaagacgaac tccgtccggt cggcgaagct cttgaaaccc tggacaatca
     2041 gcttcttcaa acgcatggcg tgtggtcctc catgaccgcg agggcaaatg ctgaaggatg
     2101 aatgcggaat gcagaatgaa gaccaacgac gtcagctctt atggttttca ttctgcattc
     2161 tgcattctgc attcatcatt ctgcattcgc ccatcacttc ccatttgacg cttccagttc
     2221 ggccgatccc acctgtgccg gacgctgctc cgcagcggcg ggcgtcgggg ttgcctgctc
     2281 tttcggtacg ttcggtcgct cgctttgcgg gcggccgttg tcgcgcaaca acggagccgt
     2341 atcgactgat gccgttgcct caacggccgg cggcccttgt aacacaaaag cggccaattg
     2401 tcgctgggtg ccgcacaatc ctgacacttt ctgctgctcg accatcccct gcacgatggc
     2461 gacgac
//
LOCUS       DATIMH010001059         5376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_832843, whole genome shotgun sequence.
ACCESSION   DATIMH010001059 DATIMH010000000
VERSION     DATIMH010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832843"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VLI90_18355"
     CDS             <1..765
                     /locus_tag="VLI90_18355"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-quinone oxidoreductase"
                     /protein_id="HSV16231.1"
                     /translation="YGLDMGAFTPFLYAFREREYILDLFEQLCGARLTLSYINSGGVT
                     WDLPPQFLEKLTEFLDYFEPKIDEYNDLLSFNHIFVKRTANVGVISKDLAVTYALSGP
                     VIRGSGIPLDLRRDRPYSIYDRLEFDVCVGDGRMGTLGDCWDRYWVKMQEMKQCIRIL
                     RQCIKQVPDGRFRAKLPRAMKVPPGEVYEEYENPRGHLGFYIESQGGTIPYRVKIRGP
                     SFVNLAVTGELCRNVLLADVPAIVGSIDIVMGEVDR"
     gene            887..1342
                     /locus_tag="VLI90_18360"
     CDS             887..1342
                     /locus_tag="VLI90_18360"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HSV16232.1"
                     /translation="MPDDGDITQLHGHGIIAGYGVPGRAVAEWLKSHGIPYVVIEANE
                     KIVNRCVKTGVRMLYGDVRNEQMLRNAGIEHASLFAVAVPAEAASLEAVALARRLNPN
                     VRIIARCTYISGGLEATRRGAEETIVAEEVVASEFVRRLQAHPVPADAK"
     gene            1607..1960
                     /locus_tag="VLI90_18365"
     CDS             1607..1960
                     /locus_tag="VLI90_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16233.1"
                     /translation="MAKQWYDVLPTHDVRMAMAWLQNDRDVSVFVTEQVDHAFEGSSL
                     LEKVRTLHPKVRRIVLTNYSDLSRLIEGLHNGAIERLVRKPIDRQEFLAAIAPSDGHI
                     AAIATTGTAKIARSA"
     gene            2034..2948
                     /locus_tag="VLI90_18370"
     CDS             2034..2948
                     /locus_tag="VLI90_18370"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSV16234.1"
                     /translation="MARRLRRSAEEQLADQIASTLLSTRQGRQFLLVAIIVGVITFGA
                     YWLWQNQLRPRHPVGPTVRLATWNLRQFSESRKHVDLQRIAGIIRDNHFDLIAIQEVK
                     RDGEEVDRLLSVLGSPWRAARFSDTSGNYERFAFIYNADHVQEIGTPHFIATTDAVIF
                     DRTPYQDTFKAGNFAFTLIEVHLSYTNTTRRRQEAEALAHFAKRLKETSAEKDVIVCG
                     DFNEQPPNGNLHYFLDQGWTSLNGDPTNLGSTEIYDTFLIDPQQTKQWNGVAGSVHFD
                     EQLYGNDDKQAEEEISDHRPAYADFATN"
     gene            complement(3106..4077)
                     /locus_tag="VLI90_18375"
     CDS             complement(3106..4077)
                     /locus_tag="VLI90_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16235.1"
                     /translation="MPAAVDPAHATSAPDWTAIVDEVFCPLCDYNLRGLTEPRCPECG
                     YQFAWPELLDRRLREHPFLFEHHPRRNIVSFVKTLLAGWMPRRFWRTLTARHEIHPRR
                     LRWYAVLAIVMPFLLAGTGLFSFSAWQTATKNAQRAVVAPRLLSGGGYGYMYKNGELW
                     IQAYHPFSKRYAFERVNPPWNSFDFAAEAFERMAIPLRFPAAVLALLAWPCVTFATLM
                     IFRDSMRLANVKRMHVIRCVVYSFDCIAIVAIASLLWRPSLQTLVSNDTFDMSIGPML
                     MLFPLAWYRLGSAYSRYLRFDRPYATALASQVITFLAIAAVVVYAVE"
     gene            complement(4261..5283)
                     /locus_tag="VLI90_18380"
     CDS             complement(4261..5283)
                     /locus_tag="VLI90_18380"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HSV16236.1"
                     /translation="MPTMRAVQVSRAGGALELVQRPIPEPLDASVRIKVQACGICHSD
                     ALTVEGHMPGIEYPRVPGHEVVGVIDAVGANVPPRWAVGMRVGIGWHAWHCGFCDACR
                     RGDFFACHNGVKVTGISFDGGYADYMIAPWTALAVMPSELPAADAAPLMCAGLTTFNA
                     LRNSGAVAGDVVAVLGLGGLGHLGVQYAAKMGFRTVAIARGADKAPLAKQLGATEYID
                     SQASDPAAELMKLGGAKVVLATVTVGSAMSATVAGLAPRGRLMVLGAADNMAVSPLQL
                     LMARRSIEGWYSGTSIDSEDTLAFSMQSGVRSMNEVYPLERAADGYARMMSGKARFRV
                     VLTTGN"
BASE COUNT         1049 a         1689 c         1712 g          926 t
ORIGIN      
        1 tacgggctgg acatgggcgc gttcacgccg ttcctgtacg cgtttcgcga acgcgaatac
       61 atcctcgacc tgttcgaaca attgtgcggc gcacggctga cgctcagcta catcaacagc
      121 ggcggcgtga cgtgggattt gccgccgcag tttctggaga agctcacgga gttcctcgat
      181 tacttcgagc cgaagatcga cgagtacaat gacctgctga gcttcaacca catctttgtg
      241 aagcgcacgg cgaacgtggg ggtgatcagc aaagatctgg cggtgacgta cgcgctgtcg
      301 ggcccggtga tccgcggcag cggcatcccg ctcgacctgc ggcgcgatcg gccatacagc
      361 atctacgacc ggctcgaatt tgacgtgtgc gtcggcgacg ggcgcatggg cacgctgggc
      421 gattgctggg atcgatattg ggtgaagatg caggagatga agcagtgcat ccggatcctg
      481 cggcagtgca tcaaacaggt gccggatggc cgatttcgag ccaagctccc gcgggcgatg
      541 aaggtcccgc cgggcgaggt gtatgaggaa tatgagaacc cgcggggaca tctgggtttc
      601 tacatcgaaa gccagggcgg gacgattccg tatcgcgtga agatccgcgg gccgtcgttt
      661 gtgaacctgg cggtgacggg tgaactgtgc cgcaacgtgc tgctggcgga cgtgccggcg
      721 attgtcgggt cgatcgatat cgtgatgggt gaggtggatc ggtagtaaaa tccgccgcgc
      781 agcgatagcc tcgaaggggc acgtgttttc gcgctcgaac gaaaaacgcg tgtcccttcg
      841 gggctaccgc tacgcggaag agatgcgctt gggaggaacg acaggcgtgc ccgacgacgg
      901 tgacatcaca caactgcacg gtcacggcat catcgccggg tacggcgtgc ccgggcgcgc
      961 tgtcgccgag tggctcaaga gccacggcat tccatacgtc gtcatcgaag ccaacgagaa
     1021 gatcgtcaac cgctgcgtga agaccggggt gcgcatgctg tacggcgacg tgcgcaacga
     1081 acagatgctg cgcaacgccg gcatcgaaca cgcgtcgctc ttcgccgtcg cggtcccggc
     1141 agaggcggcg tcgctggaag cggtggcgct ggcgcgccgg ctcaatccga acgtgcgcat
     1201 catcgcgcgt tgcacctaca tctctggtgg tctagaggcg acccgccgcg gcgcggaaga
     1261 gacgatcgtt gccgaggagg tcgtcgccag cgaattcgta cgccgcctcc aggcccatcc
     1321 ggttcccgct gacgcgaaat gaactgcgcg cgacagggct tgcgcgctcg ctcgcatcag
     1381 attcgcgcac agaatcccac gatcactctc aagaaaacgg cgcgtgcgtt cgatacatca
     1441 gttagttgcc gccgcgcgcg gtgtgcgtgg atggctgtgt gcacctcctg gtcacgggaa
     1501 cacgattacg gctttttcgg atttgctcgt catgatccct tcgggtgaga agcgaaaact
     1561 cgttgtcctc gacgccgacc ggcggaccct tcatgacgta tgtgacgtgg ccaagcaatg
     1621 gtacgacgtc ctgccgacgc acgatgtgcg gatggcgatg gcgtggctgc agaacgaccg
     1681 cgacgtttca gttttcgtca ccgagcaggt ggaccacgcc ttcgaaggca gcagcttgct
     1741 cgagaaggtc cgcacgcttc accccaaggt tcggcggatc gtactaacca attattcaga
     1801 cctgagccgg ctcatcgaag gcctgcacaa tggggctatc gagcggctgg ttcgcaagcc
     1861 gatcgaccgc caggaattcc tcgccgccat cgcgcccagc gacggtcaca tcgccgccat
     1921 cgcaaccacc ggcaccgcga aaatcgcgcg cagcgcgtaa ttaatagact tgcgcaaatt
     1981 cgggctgaag aggcatcaac acgcgcctcc cttcgcgcga tatactcccc ctcatggcac
     2041 ggaggctgcg gcggagcgcc gaggaacaac tcgccgatca aatcgcttcg acacttctca
     2101 gcacccgtca gggccggcag ttcctgctcg ttgcgatcat cgtcggcgtc atcacgttcg
     2161 gcgcgtattg gctctggcag aaccagctgc gcccgcggca tccggtgggt cccaccgttc
     2221 gcctggcgac gtggaacctg cggcagtttt ccgagagccg aaagcacgtt gacctgcagc
     2281 gcatcgccgg catcatccgc gataatcatt tcgatctgat cgccatccag gaagtgaagc
     2341 gcgacggcga agaggtggat cggctgctga gtgttttagg ttcaccctgg cgagcagcgc
     2401 gcttcagcga caccagcgga aattacgagc gcttcgcgtt catttataat gccgatcacg
     2461 tgcaggagat cggcacgccg catttcattg cgacgaccga tgcggtcatc ttcgatcgca
     2521 cgccgtacca ggataccttc aaagccggca acttcgcctt cacgctgatc gaggtgcacc
     2581 tcagctacac caacacgacg cgccgccggc aggaggccga ggcactggcg cacttcgcca
     2641 agcggctgaa agaaaccagc gctgagaaag acgtgatcgt ctgcggcgac ttcaacgagc
     2701 agccgcccaa tgggaacctg cactacttcc tcgaccaggg ctggacaagc ctgaacggcg
     2761 acccgacgaa tctcggcagc accgaaatct acgacacgtt cctgatcgac ccgcagcaga
     2821 ccaagcagtg gaacggcgtc gccggcagcg tgcatttcga cgagcagctg tatggcaacg
     2881 atgacaagca ggcggaagaa gaaatcagcg atcaccggcc ggcgtacgcg gattttgcga
     2941 cgaattagca attcatcctg agcgaaggcg cactcgccgg agtcgaagga tctggccgag
     3001 aagtaagtga agcgtccctc ctcaagacct gccgcttgcg gcttagcgtc tttcaattcg
     3061 cagataaaac atgctaagcc gcaagcggct gcggatgaat gattgttact ccaccgcgta
     3121 aaccacaacc gccgcgatgg ccaggaaggt gatcacctgc gacgccagcg ccgtcgcata
     3181 tggccgatcg aagcgcaggt atcggctgta cgccgatccc aagcggtacc acgccagcgg
     3241 gaacagcatc agcatcgggc caatgctcat gtcgaacgtg tcgttcgaca ccagcgtttg
     3301 cagcgatggt cgccacagca gcgaggcgat ggcgacgatc gcgatgcagt cgaagctgta
     3361 gacgacgcag cggatcacgt gcatgcgttt cacgttcgcc agccgcatcg aatcgcggaa
     3421 gatcatcagg gtcgcgaacg tcacgcatgg ccaagccagg agcgccagca ccgccgcggg
     3481 aaagcgcaat gggattgcca ttcgctcgaa cgcttcggcg gcgaaatcaa aactgttcca
     3541 cggcggattc acgcgttcga aggcatacct tttggaaaag ggatgatagg cctggatcca
     3601 cagctcgcca tttttgtaca tgtagccgta cccgccgccg gacaacaggc gcggcgctac
     3661 gacggcgcgc tgtgcatttt tggtcgccgt ttgccacgcg gagaaggaaa agagaccggt
     3721 gccggcgagc aggaacggca tcacgatcgc gagcaccgcg taccaccgca gtcgccgcgg
     3781 gtggatctcg tgtcgcgccg tcagcgttcg ccagaaccgc cgcggcatcc agccggccag
     3841 cagcgtcttg acgaacgaca cgatgttccg gcgggggtga tgctcgaaca gaaacgggtg
     3901 ctcgcgcagc cgccggtcca gcagctcggg ccaggcgaac tgatacccgc actcggggca
     3961 tcgcggctcg gtcaagccgc gcaggttgta atcgcacagc gggcagaaaa cttcatcgac
     4021 gatcgccgtc caatcgggtg ctgaagtcgc atgagcagga tcgaccgccg cgggcatggg
     4081 tgcaattatg gacggccgcg gggggcagat accagtggct aaattgcggt gtagaactac
     4141 cgcggacgat gcgcgcaggg agagccggcc cgtccccggg ccgttccggg tgttcgcggc
     4201 cgttccgggc cggggatggc ccggctctcc ttggcgcatc gagcggtgat tgattatcga
     4261 tcagttcccg gtcgtcagca ccacgcggaa tctcgctttg ccgctcatca tccgcgcgta
     4321 tccgtcggcc gcgcgctcca gcggatagac ctcgttcatc gatcgcacgc cggattgcat
     4381 gctgaaggcc agcgtgtctt ccgagtcgat cgacgtgccg gagtaccagc cttcgatcga
     4441 ccggcgggcc atcagcagtt gcaggggaga caccgccatg ttgtcggcgg cgccgagcac
     4501 catcaaccgg ccgcgcggcg cgagcccggc gaccgtcgcg ctcatcgccg atccgaccgt
     4561 gaccgttgcc agcacgacct tcgcgccgcc gagcttcatc agctccgccg cggggtcgct
     4621 ggcctggctg tcgatgtact cggtcgcgcc cagctgtttg gcgagcggcg ccttgtccgc
     4681 gccgcgcgcg atggcgacgg tgcggaagcc catcttcgcc gcgtattgca cgcccaggtg
     4741 gccgagcccg cccaggccca gcaccgcgac gacgtccccg gcgaccgcgc cgctgttacg
     4801 cagcgcgttg aacgtcgtca gccccgcgca catcaacggc gccgcgtcgg cggcgggcag
     4861 ttcgctcggc atcaccgcca gcgccgtcca cggcgcgatc atgtaatccg catatccacc
     4921 gtcgaacgag atgcctgtga ccttcacgcc attgtggcac gcgaagaaat ccccgcggcg
     4981 gcaggcgtcg caaaacccgc agtgccacgc gtgccagccg atgccgaccc gcattcccac
     5041 ggcccatcgc ggtgggacgt tcgcgccgac ggcgtcgatc acgccgacca cttcatgccc
     5101 gggcacgcgc gggtattcga tgccgggcat gtggccttcg acggtcagcg catcgctgtg
     5161 acagatgccg caggcctgca ccttgatccg cacactggcg tcgagcggct cgggaattgg
     5221 ccgctggacg agttccaaag ctccgcccgc acgtgagact tgaacggcac gcatggtagg
     5281 catggggtaa ctcctgttct gtaatggtcg gccgcggttt cgtggatgat aggcgcgcgg
     5341 ttttggtgat cgcgtagcgg tagccccgaa ggggca
//
LOCUS       DATIMH010001060         4593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3719239, whole genome shotgun sequence.
ACCESSION   DATIMH010001060 DATIMH010000000
VERSION     DATIMH010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3719239"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VLI90_18385"
     CDS             complement(<1..1071)
                     /locus_tag="VLI90_18385"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HSV16237.1"
                     /translation="MTILTALLAVHASAAPDLEIMNVPAAADAGIIAIASDEHVASII
                     IDPQDFPVVHLAAGFFADDVERMSGHRPLVGEAPVGDNPVIIVGTIGHSAIIDRLARQ
                     GKLPELDKIKGHWETTLWAVVENPMPNVARALVIGGSDRRGTAYGLMQLSERIGVSPW
                     YWWADVPAQHRDSLAVRIASPQVDSPGVKYRGIFINDEDWGLNPWAKPTFDPEFKNIG
                     PKTYEKVFELMLRLRLNYIWPAMHACSTEFGSMPENVALADKYGIVAGASHCEPMLCN
                     NVKWDVNAKGPWDYSKNRDVIRSYWEDMVKARGDDEAVWTLGIRGIHDAPMNGPKDIP
                     ARVKLVTEVIHDQRELIDNYVSP"
     gene            complement(1147..3378)
                     /locus_tag="VLI90_18390"
     CDS             complement(1147..3378)
                     /locus_tag="VLI90_18390"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16238.1"
                     /translation="MNCFTRRALAILFFVSGFSSLVYQIVWTRLAFASFGIIAPVLSI
                     VLSVFMLGLALGSWAGGRMIVPTSRQTGRSAIYFYAAAEMLIGVGAFIVPMLFKTSER
                     LLAGVGQLNSFSYLFYSALALGIAIFPWCVLMGTTFPFMMAFVKEKDAKRSDSFSFLY
                     VANVLGAMLGTALTAIVLVEVLGFHHTLWVAAGGNFLIALTSSWLGVAEGASPQPREG
                     DPSEDSGAATRTLVTSDRLADPLSKSLLFSTGFLSMAMEVVWSRGFVPVLKTQVYSFA
                     LILFAYLLATAAGSLLYRAAVRRGRFFSYGNIFFWMAVAALLPVFLLDPTVTVQLYTY
                     GAIDPPSAILILASILPLCALLGYLTPRLIDQYSSGRPGRAGAAYAANVVGCILGPLC
                     ACYLLLPHISTRYSLILLGCPFIAFWLIVSKPKQYQYKMMGGLLLAAVIVRSLLYTAD
                     FDEVILKQPHGAVDRDYIASVATFGDDRLTKTLLVNGMGMTVLSPITKIISHLPLALH
                     RDPPHSVLVVCFGMGTSYRSALTWDVDVTAVELVPSVPKRFGFFHADAKEYVDNPRGH
                     IVIDDGRRYLSRCGRKFDVIVVDPPPPIEAAGSSLLFSTGFYALAKQHLNPGGIVQIW
                     YPGGGDKTTDQAVVRSMYDSFPFVRGFVSIEGWGMHLLGSMEPIEVPPIDRVVARLPP
                     AAKVDLVEWTPGVDATGLMTSVLRRQLRVEQMLSPDPSIEITDDRPMNEYYLLRDLKK
                     NPD"
     gene            3657..>4593
                     /locus_tag="VLI90_18395"
     CDS             3657..>4593
                     /locus_tag="VLI90_18395"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HSV16239.1"
                     /translation="MPIMTGLSGNEIFCLNLKGFSPGELVIGNSVFSMGFAGAVGAIG
                     RGLMGGEVTEVTNIIHEGRIEAYVRMAREAERHGGVGITGVTNELRTFHGHTEFLSVA
                     STLHRHNAGRNETIDFSTSADGQELYCQLDAGFTPVKFVFGNVAYSVGVAGGILGKLK
                     SMARGEIKEFSDVFNRTRHLALDRIVAEARAAGADAVVGIETRTLPFAGVHEMLMMGT
                     ASRHPALRVVQAQPDNTIAYQSAPATTKHAAYPGQPVSSDLTNEEMWNIVHMGYMPLK
                     LVLGTAVYSLGVIGGIKAMLRGFARGEIPELTSLIY"
BASE COUNT          973 a         1457 c         1351 g          812 t
ORIGIN      
        1 cggcgacacg tagttgtcga tcaactcgcg ctgatcgtga atcacttccg tcaccagctt
       61 cacgcgggcg ggaatatctt tgggcccatt catcggcgca tcgtgaatgc cgcggatgcc
      121 cagcgtccac acggcttcgt cgtcgccgcg ggccttcacc atgtcttccc aatatgagcg
      181 aatgacgtcg cgattcttgc tgtaatccca cggccccttg gcgttcacgt cccacttcac
      241 gttgttgcac agcatcggct cacagtgcga cgcgccggcg acgatgccat acttgtccgc
      301 cagcgccacg ttctccggca tcgagccgaa ctcggtgctg cacgcgtgca tggccggcca
      361 gatatagttc agccgcaggc gcagcatcag ctcgaaaact ttttcgtaag tcttgggacc
      421 gatgttcttg aattcaggat cgaacgtcgg cttcgcccag gggttcaggc cccagtcttc
      481 atcgttgatg aagatgccgc ggtatttgac gcccggcgaa tcgacctgcg gcgacgcgat
      541 gcgcaccgcc agtgaatcgc ggtgttgtgc cggcacgtct gcccaccaat accacggcga
      601 cacgccaatt cgctccgaca gctgcatcag cccataggcg gtgccgcgcc gatcgctgcc
      661 gccgatgacc agcgcccgcg cgacgttggg catcggattc tcgaccaccg cccaaagcgt
      721 tgtttcccag tgacctttga tcttatccag ctccggcagc ttcccctggc gcgcgaggcg
      781 atcgatgatc gcgctgtggc cgatcgttcc gacgatgatc acgggattgt ccccgaccgg
      841 cgcttcgccc accaacggcc ggtgaccgct catgcgctcc acatcatcgg caaagaatcc
      901 cgcggcgaga tgaacgactg gaaaatcctg cgggtcgata atgatcgagg cgacgtgctc
      961 atcggatgca atggcgatga tgcccgcatc ggcggcagcc gggacgttca taatttccaa
     1021 atccggcgct gcgctcgcat gaacggcgag tagcgctgtg agtatggtca tgcagaagag
     1081 cgtcttcata tgcttcaata tcacaggagg tctccagcat ttccaccccg cactccctct
     1141 gcgggctcag tcagggttct tcttcaaatc gcgcagcaag tagtactcgt tcatgggccg
     1201 atcatccgtg atttcgatgc tcgggtcggg gctgagcatc tgttcaaccc ggagttgacg
     1261 cctgagcacg gacgtcatta aacccgtcgc gtccaccccc ggcgtccact ccaccagatc
     1321 taccttagcc gcggggggca ggcgcgccac cactcgatcg atcggtggca cttcgatcgg
     1381 ctccatcgag cccagcaggt gcattcccca gccttcgatc gatacgaacc cgcgcacgaa
     1441 cgggaacgaa tcatacatcg atcgcacgac ggcttgatcg gtcgtcttgt caccgccgcc
     1501 gggataccat atctgcacga tgccgccggg gttcaggtgc tgttttgcca gcgcgtaaaa
     1561 cccggtggaa aagagcaggc tggaaccggc cgcttcgatc ggcggcggtg gatcgacgac
     1621 gatcacgtcg aacttcctgc cgcatcggct caggtatcgg cggccatcgt cgatgacgat
     1681 gtgcccgcgc ggattatcga cgtattcctt cgcatcggcg tggaagaagc caaaccgctt
     1741 gggcacgctc gggaccagtt ccaccgcagt gacgtcaaca tcccaggtca gggccgaccg
     1801 gtaagacgtg cccatcccga agcagaccac caggaccgaa tgcggtggat cccgatgcag
     1861 cgccagcgga aggtgcgaga tgatcttggt gatcgggctg agcaccgtca tgcccatgcc
     1921 gttcaccaac agcgtttttg tgagccgatc atcgccaaac gtcgcaaccg aagcgatgta
     1981 gtctcggtcc accgcgccat ggggttgctt gagaatgact tcgtcgaaat cggctgtgta
     2041 aagcaaagag cgcacaatca ccgcggccag aagcaggcca cccatcatct tgtattggta
     2101 ctgcttcggc ttcgacacga tcaaccagaa cgcgatgaaa gggcagccca gcagaatcaa
     2161 tgaataccgc gtgctgatgt gcggcaacaa aagataacat gcacacagcg gcccgaggat
     2221 gcagcccacg acgttcgctg catacgccgc tccggcacgt ccgggacggc cgctggaata
     2281 ctggtcgatc aggcgaggcg ttagatatcc gagtagcgcg cacagcggca agatgcttgc
     2341 gaggatcagg attgcgctgg ggggatcgat cgcgccgtac gtgtaaagtt gcaccgtcac
     2401 ggtcggatcg agcaggaaca ccggcagcag tgcggccacg gccatccaga agaagatgtt
     2461 accgtaagaa aagaatcggc cgcgccgcac cgccgcgcga tacagcaaag aacccgccgc
     2521 cgttgccagg aggtatgcga acaagatcaa ggcgaatgaa tagacctgcg tcttcaacac
     2581 aggcacgaac ccccgcgacc acaccacttc catggccatt gagaggaagc cggtggagaa
     2641 caacaaggac ttgctcaacg gatccgccag gcgatcggaa gtgaccagcg tccgcgtcgc
     2701 tgcgccggaa tcctccgatg gatcgccttc tcgcggttgc ggcgacgccc cttcggcaac
     2761 gcccagccaa ctgctggtca acgcgatcag gaaattacca cccgcggcaa cccaaagcgt
     2821 gtgatgaaat cccagcacct ccaccagaac gatcgccgtc agcgctgtcc cgagcatcgc
     2881 gcccagcacg ttggccacgt agagaaagct gaagctgtcc gatcgcttcg catccttctc
     2941 tttgacgaag gccatcatga acgggaaggt cgtccccatc agcacgcacc agggaaagat
     3001 cgcaatcccc agcgccagtg ccgaataaaa cagatagctg aatgagttga gttggccgac
     3061 gccggcgagc aggcgctcgc tggtcttgaa caacatcggc acgatgaacg cgccgacccc
     3121 gatcaacatc tccgcggccg catagaagta aatcgcagaa cggccggtct gccgggaagt
     3181 tggaacgatc atccgcccgc cggcccacga gcctaatgcc agaccgagca tgaagaccga
     3241 cagcacgatt gacagcaccg gcgcgatgat gccgaacgag gcaaacgcca ggcgcgtcca
     3301 gacgatctgg taaaccaaac tggaaaaccc ggagacaaaa aataggatcg ccagcgcccg
     3361 ccgcgtaaag caattcatca gacccgcatc ttaacggacg agccgacact tttcgcatct
     3421 gtctcgcctc atgaacattg agcggttcca ccgtgacacg ggcccccggc tgccccgccc
     3481 cgtgcgcaaa acgtccaaac gtcagagact gttggttctg ctgcaagcgc ctctcgctcg
     3541 gccggagccc ctgtgtcacg gctagattct cgctgcagtt tataaggcta tcgcgccctg
     3601 gacgatgtta atatgcccgc cacggccccg agcaatcgaa cggagacaac acgcccatgc
     3661 ccatcatgac cggcctttcc gggaacgaaa tcttctgcct gaacctcaag ggcttctcgc
     3721 ccggcgaatt ggtcatcggc aacagcgtgt tctcgatggg attcgcgggc gcggtcggcg
     3781 cgatcggccg cgggctgatg ggcggcgagg tgacggaggt caccaacatc atccacgagg
     3841 gccgcatcga ggcatacgtg cggatggcgc gcgaggccga gcggcacggc ggcgtgggca
     3901 tcaccggtgt gaccaacgag ctgcgcacgt ttcatggaca tactgagttt ctatcagttg
     3961 catcaacgtt gcaccggcac aatgccgggc ggaatgagac gatcgacttt tcgaccagcg
     4021 ccgatggtca ggaactgtat tgccagctcg atgcggggtt cacgccggtg aagtttgtct
     4081 ttggcaacgt cgcgtattcc gtcggtgtgg ccggcggaat cctggggaag ctaaagtcga
     4141 tggcccgcgg ggagatcaag gagttcagcg acgtcttcaa ccgcacgcgg cacctggcgc
     4201 tggaccgcat cgtggccgag gcgcgcgcgg ccggcgcgga tgccgtggtc ggcatcgaga
     4261 cgcgcacgct gccgttcgcg ggcgtacacg agatgctcat gatggggacc gcgtcgcgcc
     4321 acccggcgct gcgcgtcgtg caggcgcagc cggacaacac gatcgcgtat caatccgcgc
     4381 ccgcgacgac caagcacgcg gcgtatcccg gccagcccgt cagcagcgat ctaactaacg
     4441 aagaaatgtg gaacatcgtg cacatgggct acatgccgct gaagctcgtg ctcggcacgg
     4501 ccgtctattc gctcggtgtg atcggcggga tcaaagcaat gctccgcggc tttgcccgcg
     4561 gtgaaattcc cgaactgacc agcctgatct acg
//
LOCUS       DATIMH010001061         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4296672, whole genome shotgun sequence.
ACCESSION   DATIMH010001061 DATIMH010000000
VERSION     DATIMH010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4296672"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VLI90_18400"
     CDS             <1..1322
                     /locus_tag="VLI90_18400"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSV16240.1"
                     /translation="LEKRYTELGASITVITKPFSNHHPHSLRNPTPIVNFVLAHTGGP
                     TDAPVKTPGTPYGYDYFVLRSSLDKSRLKFERDKTGRVAFLGGSITNMKGWRDLVCQD
                     LQRRFPDAKFDFINAGIPSTGSMPGAFRFTRDVLAHGPVDLLFEEAAVNDEVNGQSTP
                     EQVRGMEGIIRQARLANPLTDIVMLHFVDPPKMEVIRHGDTPAVIVNHEKVAAAYAVP
                     SIDLAQEVTERIQAGEFTWEKDFQNLHPAPFGQQLYAKSIGRLLDAAWAKPLAADAQP
                     QAYAVPAEMVDRASYDHGRLVAPSEASHDDAWQLVENWKPTDGAGTRPGFVNVPALVA
                     EKPGATLHLKFNGNAVGVFAPAGPDAGVIEFSIDGGSTSSRDLYTKWSGHLHLPWATV
                     LAAGLPPGEHALLLRVASNRNEKSKGTAVRIVHFLVNGPSGKETPTP"
     gene            1319..>2305
                     /locus_tag="VLI90_18405"
     CDS             1319..>2305
                     /locus_tag="VLI90_18405"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HSV16241.1"
                     /translation="MSMPTHAKSFRRAIGVTAAALVFAAGCASSNNNQPQDETPAAGA
                     VVTTLAPSAAAPAAAEKAPKETTAAAAPATQPVYSVPYRIPKVDEVTATLQHLRDRLD
                     AWAPVPQGGATPAPTERHQGLIGYPIGVAYAGMIAAYEATGDKAFADFDAKRFEWIAE
                     QVKRQTENPPPDEQPVAGRPLRNPVRNLLKPNSLDSCGAMNAAFVKAKRANIGPDMDK
                     AIQIASDWVSHKQFRLQDGTLARNRPFKNSLWGDDMFMGTSLLGQMGALSGDRSYFDD
                     AAKNVVQMSQRLFIPSAGLFTHAWNAETGDNQPTYFWGRANGWNIVAMAELLS"
BASE COUNT          450 a          732 c          756 g          367 t
ORIGIN      
        1 tgctcgagaa gcgttacacg gagctgggag catcgatcac cgtcatcacc aaaccattca
       61 gcaatcacca tccgcacagc ctgcggaacc cgacgccgat cgtgaacttc gttctcgcgc
      121 acaccggcgg gccgacggat gcgccggtga agacgcccgg cacgccgtat gggtacgact
      181 acttcgtcct gcgttcatcg ctggacaaga gccggctgaa gttcgagcgc gacaagaccg
      241 gtcgcgttgc gttcctcggc gggtcgatca ccaacatgaa aggctggcgc gacctggtgt
      301 gccaggacct gcagcggcga ttccctgacg cgaagttcga cttcatcaac gcgggcattc
      361 catcgacggg ttcgatgccg ggcgcgtttc ggttcacgcg cgatgtgctg gcgcacgggc
      421 cggtcgatct gctgttcgaa gaggcggccg tcaacgatga ggtgaacggc cagagcacgc
      481 ccgagcaggt acgcggcatg gaaggcatca tccgccaggc gcggctggcg aatccgctca
      541 ccgacatcgt catgctgcac ttcgtcgatc ctccgaagat ggaggtcatt cgccacggcg
      601 acacgccggc cgtcatcgtc aaccacgaga aggtcgcggc ggcgtacgcg gtgccgtcga
      661 tcgacctggc gcaggaagtg accgagcgaa tccaagccgg cgagttcacg tgggagaagg
      721 attttcagaa cctgcatccg gcgccgttcg ggcaacagct gtacgcgaag tcgatcggcc
      781 gattgctcga cgcggcgtgg gcgaaaccgc tggcggcgga cgcgcagccg caagcatatg
      841 cggtgccggc ggaaatggtc gatcgcgcca gctacgacca cggtcgattg gtcgcgccgt
      901 ctgaagcatc gcacgatgac gcgtggcagc tggttgagaa ctggaaaccg accgacggcg
      961 ccggcacgcg gccgggattc gtcaacgtcc cagcgctggt cgccgaaaaa ccgggcgcga
     1021 cgctgcacct gaaattcaac ggaaacgccg tcggcgtgtt cgccccggcc ggccccgatg
     1081 ccggcgtcat cgaattcagc atcgacggcg gttcaacgtc cagccgcgat ctgtatacaa
     1141 agtggagcgg tcacctgcat ctgccctggg cgaccgtgct ggcggcgggt ttgccgccgg
     1201 gagaacatgc tctgcttttg cgcgttgcaa gtaatcgcaa cgaaaagagc aaagggacgg
     1261 ctgtccgaat tgttcatttc ctcgtcaacg gaccttcggg aaaggaaacg cctacgccat
     1321 gagtatgcca acgcacgcca agtcattccg tcgagcgatc ggtgtcaccg ccgcggccct
     1381 ggtgttcgcc gccggttgcg cgtcatcgaa caacaaccaa ccgcaggatg agacgcccgc
     1441 ggcaggcgcc gtggttacaa cgctggcgcc aagcgccgcc gcccccgccg ctgcggagaa
     1501 agcaccgaaa gaaacgactg ccgcggccgc gccggcgact cagccggtgt attcggttcc
     1561 ctatcgcatc cccaaggtgg acgaggtgac ggcgacgttg cagcacctgc gcgatcggct
     1621 cgacgcgtgg gcgccggtgc cgcagggcgg agcgacgcca gcgccgacgg agcggcacca
     1681 ggggctgatc ggttatccga tcggcgtcgc ctacgccggg atgatcgcgg catacgaagc
     1741 gaccggcgac aaggcgttcg ccgacttcga cgccaagcgg ttcgagtgga ttgccgaaca
     1801 ggtcaagcgg cagacggaga atccaccgcc ggacgaacaa ccggtggcag gccggccgtt
     1861 gcggaatccg gtgcgcaatc tgctcaagcc gaactcgctc gactcctgcg gcgcgatgaa
     1921 cgcggccttc gtcaaagcga agcgcgccaa catcgggccc gatatggaca aggcgattca
     1981 gatcgcgtcc gactgggtca gtcacaagca gttccgcctg caggacggaa cgctggcgcg
     2041 caatcggccg ttcaagaatt cgctgtgggg cgacgacatg ttcatgggca catcgctgct
     2101 gggacagatg ggcgcgctgt ccggcgatcg ctcgtacttc gacgatgcgg ccaagaacgt
     2161 ggtccagatg tctcagcgcc tgttcattcc cagcgcgggc ctgttcacgc acgcgtggaa
     2221 cgccgagacc ggcgacaatc agcccactta tttctggggc cgtgccaacg gttggaatat
     2281 cgtcgcgatg gccgagttgc tcagc
//
LOCUS       DATIMH010001062         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2273243, whole genome shotgun sequence.
ACCESSION   DATIMH010001062 DATIMH010000000
VERSION     DATIMH010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2273243"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VLI90_18410"
     CDS             complement(<1..358)
                     /locus_tag="VLI90_18410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSV16242.1"
                     /translation="MMLDLSIVIPTVNRASLLERGLNAIRDTVKCSHEVIVVDGASTD
                     HTPQVLEQARWSFGDRLKVIREDQREGFVRAANKGFRVASGRNLTWLNDDARPLPGAY
                     DTAVSQMDAASDDVAFL"
     gene            complement(355..1557)
                     /locus_tag="VLI90_18415"
     CDS             complement(355..1557)
                     /locus_tag="VLI90_18415"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSV16243.1"
                     /translation="MIVAVDTNCILPGQVGGIENYTLGLIEALKLPDSPASKIVLLTR
                     SENQKLFQPFVDSSTEIVEVERPRHKGKAVANWSELLAKHPVGGRRTLADFQQHKTRL
                     LHQRHVDLVHFPGNTINPLDLDLPVVLNLHDLQHRHFPQYFSREEIDNREKWWVASAC
                     RADALIAASNYVRDDLQRQLRVDRRKIFVTPDVFQSAFFTPPSAAQLNDLRDRLALPE
                     TFFVYPAAVWPHKNHERLIRAFVAAEIPNAQLILTGGGQKDSALPQLIEELGAEFAVR
                     LAGRVSTDDLIGLYHLASGLVFPSEHEAWSIPVMEAMACGCPVASSNVTSLPEEIGDA
                     GLLFSPNDIDAMADALRRLASDADLRRELSQRGRDRVKQFEPDCFVRTVADAYDHARR
                     TYRSRKAA"
     gene            complement(1659..2798)
                     /locus_tag="VLI90_18420"
     CDS             complement(1659..2798)
                     /locus_tag="VLI90_18420"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSV16244.1"
                     /translation="MKINQPKHETIPWARPAIDDAEMREVVACMESGWLTSGPRVERF
                     EREMARRAGRKHAVAVSNGTAALDLALRVIGVSAGDEVVVPALSYVATASAVAIQGAD
                     PVFADVDRRNLGLDPASAADRITRRTAAIVCTDHGGNPCDYAKLTALAATTRLPLVLD
                     GAQSIGATFDGKPALSYGLVSTTSFHAAKTMTTVEGGMAFTDDESLARRMRIIRSQGE
                     DPQRKYYHIELGHNFRMTELQAAIGLSQLTKLDALLADRQALAAQYRSEFAPLGLASP
                     VGLPNARNSHFLFSLMVPQRDRIVARLKQRSVETRVCYPLPLYAQPIFERNRNHFCPV
                     TEETCAKILNPPMFYGLTLAQQQRVAAELTAAIAALAPSEMRKAV"
BASE COUNT          512 a          964 c          929 g          552 t
ORIGIN      
        1 ataggaaagc aacatcatcg ctcgccgcgt ccatctgcga caccgcggtg tcgtatgcgc
       61 cgggcagcgg tcgcgcgtcg tcgttcagcc aggtcaggtt ccgcccgctg gcgacgcgga
      121 aacctttgtt ggccgcgcgc acgaaacctt cgcgctgatc ttcgcgtatc accttcaacc
      181 ggtcgccgaa ggaccaccgc gcctgctcca gcacctgcgg cgtgtgatcg gtactcgcgc
      241 cgtccaccac gatcacctcg tgcgaacact tcacagtatc gcgaatggcg ttcagcccgc
      301 gctccagtaa agaagcgcga ttgacggttg ggatgacgat cgacaggtcc agcatcatgc
      361 cgcctttcgt gagcgatagg tccgccgggc gtggtcgtac gcgtcggcca ccgtgcggac
      421 gaagcagtcg ggctcgaatt gtttcacgcg atcgcgcccg cgctgtgaga gttcgcgacg
      481 cagatcggcg tcgcttgcca atcgccgcag ggcatcggcc atcgcatcga tgtcgttcgg
      541 tgaaaagagt agcccggcgt caccgatttc ctcgggcaaa ctcgtgacgt tcgagctggc
      601 gacggggcag ccgcacgcca tcgcttccat caccgggatg ctccaggctt cgtgttcgct
      661 ggggaaaacc agtccgctgg ccaggtgata caggccaatc aaatcatcgg tgctgacccg
      721 accggcaagg cggacggcaa attccgcccc caactcctcg atcaactgcg gcaatgccga
      781 atccttctgt ccgccaccgg tcaggatcag ctgcgcgttg ggaatctccg cggcgacgaa
      841 cgcgcgaatg agccgctcgt ggttcttgtg cggccaaacc gccgcggggt agacgaagaa
      901 tgtttccggc agcgccaagc gatcgcgcag gtcgttgagc tgcgccgccg acggcggtgt
      961 gaaaaacgcc gactgaaaca catccggcgt cacgaatatc tttcggcgat ccacgcgcag
     1021 ctggcgctgc aggtcgtcgc gcacgtaatt actcgcggcg atcagcgcat ctgcgcgaca
     1081 tgccgacgcc acccaccatt tctcgcggtt gtcgatctcc tcgcgcgaaa aatactgcgg
     1141 gaagtggcgg tgctgcaggt catgcagatt caacacgacc ggcagatcca ggtccaacgg
     1201 attgatcgtg ttgccgggga agtgcacgag atctacgtgc cgctgatgca gcagccgcgt
     1261 cttgtgctgc tgaaaatccg ccagcgtccg gcgcccgccc accgggtgct tcgccagcag
     1321 ctcagaccaa ttggcgaccg ccttgccctt gtgccgcggg cgctcgacct cgacgatctc
     1381 ggtgctcgag tccacgaacg gctgaaacaa cttctgattc tccgatcgag tcagcaggac
     1441 tattttcgaa gccggcgaat ccggcagctt cagcgcttcg atcaacccca gcgtgtaatt
     1501 ctcgatcccg ccgacctggc cgggcaggat gcagttggtg tcgacggcga cgatcatttt
     1561 ctcatccttg tcctaagcgc cacgcacgat gctcgaaaac ccttttagcg gcggcccgaa
     1621 gggccgagcg tcgagcgtcg agcggcgagc gggcgttatc aaaccgcctt tcgcatctca
     1681 ctcggcgcca gcgccgcgat ggccgcggtc agctccgcgg cgacgcgttg ctgctgcgcc
     1741 agcgtcagtc catagaacat cggcggattc aggattttcg cgcacgtttc ttcggttacc
     1801 gggcagaagt gattgcggtt gcgctcgaag atcggttggg cgtacagcgg cagcggatag
     1861 cagacgcgcg tctccacgct gcgttgcttg aggcgcgcga cgatccggtc gcgctgcggc
     1921 accatcagcg agaacagaaa atgactgttg cgggcgttgg gcagtcccac gggtgacgcc
     1981 agacccagcg gcgcgaattc actgcgatac tgcgccgcca gcgcctgccg atcggccagc
     2041 aatgcatcca gcttcgtcaa ctgcgacagg ccgatggccg cctgcagttc ggtcatgcgg
     2101 aagttgtgac ccagctcgat gtggtaatac tttcgctgcg ggtcttcgcc ttgcgagcgg
     2161 atgatgcgca tccgccgggc gagcgattcg tcatccgtga aggccatccc gccttcgacg
     2221 gtcgtcatcg tcttggcggc gtggaagctc gtggtggaaa ccaagccata gctgagcgcc
     2281 ggcttgccgt cgaaggtggc gccgatgctt tgcgcgccgt cgagcacgag cggcagccgc
     2341 gtcgtcgcgg ccaacgccgt gagcttcgcg taatcgcacg gattgccacc gtgatcggtg
     2401 cacactatcg ccgccgtgcg gcgggtgatt cggtcggcag cgctggcggg gtccaggccg
     2461 aggttgcggc gatcgacgtc ggcgaagacc gggtcggcgc cctggatggc cacggctgat
     2521 gccgtggcga cgtacgacag cgccggcacg accacctcat cgccggcgct gacgccgatc
     2581 acgcgcagcg ccaaatccag cgccgcggtg ccgttgctga cggctacggc atgcttgcga
     2641 ccggcgcggc gggccatctc tcgctcgaag cgttcgacgc gcgggccgct ggtcaaccag
     2701 ccgctttcca tgcacgcgac gacttcgcgc atctccgcat cgtcaatggc gggcctggcc
     2761 cacggaatcg tctcgtgttt cggttgattg atcttcataa tctgtgcctc acgctgcggc
     2821 tcgttcggcc gtttgtttcg ccaagtcatc cgaaatgaac gccagcaatc gccggctcgt
     2881 cgcggcatag gtaaatcgtt ccagcaccgg ttgccgcatg gatgtcgcac gccgctgccg
     2941 ctcgttttcg ctggcca
//
LOCUS       DATIMH010001063         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1701071, whole genome shotgun sequence.
ACCESSION   DATIMH010001063 DATIMH010000000
VERSION     DATIMH010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701071"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VLI90_18425"
     CDS             <1..1614
                     /locus_tag="VLI90_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16245.1"
                     /translation="SANLNVGTVTTNGTIRLESIDSSYSETMTISGTLTNASTGVIQI
                     NAGTAGSRLINGALTNQGLISFSGAIDLGGTSTITNTGTIQRTDAATSNFGVSLVSQG
                     TGLVNVAAGTIRFTGTTNVGAGAFLSGGSYHVANGATLGFGNAVSQRPTINTADIQIT
                     GTGVAEIDKLVTNRGEIELLGGNDLTVAPTGGVFTNEGIIDLSPDSIFSVTGNMNFAG
                     ASQPVIHSEIASATSFGKLTVSGTFNLDSPSSTSRFDPDLVGGYDPALGATFNVITAN
                     PVSNGFDSFQGGLTPSNNVLELTRPDANTVQVKITAGPMPPAPQILNESYDFQTREAI
                     VFTFDQDVSAFLSRKDYLLQNLTTAQTVPSSAGTLSYNLNSNQATLTLTNQLPDGNYK
                     LTVAASDIANAAGEPASGAPIILNFFVLGGDATRDSKVDVGDLGVLATNYGLSGKTYS
                     QGDFNYDTKVDVGDLGILATNYGKSLAAGSVFSQIAVSSVATATSSRRTAAAEPAAAA
                     SVFATVPLTDAAWDAAGRRHERLKDVGLA"
     gene            1918..>2843
                     /locus_tag="VLI90_18430"
     CDS             1918..>2843
                     /locus_tag="VLI90_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16246.1"
                     /translation="MSRHLRVPRKRPHKSVLSAAVINTAEWLEPRRLLSSAPVGPEIP
                     IAASSGGTSLTGPAVAIDNAGDFIVTWSASGGGSNGLDIWARRYDAAGDPLGDAFVAN
                     TTTAGDQVAPAIATDAGGDFVISWATIFGNPSNELWTLSSQSFDSTGAARGGQVTLSN
                     SGWLQPGAKVTMFSDASFVAAWQTKIPGNTNEVVYQDYSSSGVPSGSQIVVTSLTASI
                     VVGVVNDASGDIAVAWLNANTAFHLSAYNGTGTALSGDVLVASANIITDAGVAMTSTG
                     NIGVTWLSSSSSSVYQCFANGFSLGGVAQGAN"
BASE COUNT          581 a          907 c          838 g          517 t
ORIGIN      
        1 agcgcgaact tgaacgtcgg caccgtcacc accaacggca cgattcgcct tgaatcgatc
       61 gatagctctt acagcgaaac gatgacgatc agcggcacgc tgacgaatgc atcgaccggc
      121 gtgatccaga tcaacgcagg caccgcggga tcgcgcttga tcaacggcgc gctgacgaac
      181 caagggttga tcagcttcag cggcgcgatc gatctgggtg gcaccagcac gatcaccaac
      241 accggcacca ttcagcggac cgatgcagcg acgagcaatt tcggcgtgag cttggtgagc
      301 cagggaaccg gtttggtcaa tgtcgcggcc ggcacaatcc gcttcaccgg caccaccaac
      361 gtcggcgccg gggcattcct ttccggcggg tcataccacg tcgccaatgg cgcgacgctt
      421 ggctttggca atgccgtttc gcagcggccg accatcaaca ccgccgacat ccagatcacc
      481 ggaactggcg ttgccgagat cgacaaactc gtcaccaacc gcggcgagat tgaattgctc
      541 ggcggcaacg acctcacggt cgcgccgacc ggcggcgtgt tcaccaatga gggcatcatt
      601 gatctatctc ccgattcgat cttctccgtc accggcaaca tgaacttcgc cggcgccagc
      661 cagccggtaa tacacagcga aatcgcatca gccaccagct ttggaaaact caccgtcagc
      721 ggcacgttca atctggattc gcccagttcg accagccgct tcgacccgga tctggtcggc
      781 ggatacgacc cggcgctggg cgcaacgttc aacgtgatta ccgcaaatcc agtcagcaat
      841 ggcttcgaca gcttccaagg cgggctcacg ccatcgaaca acgtgctcga gctgacccgg
      901 cccgacgcga acacggtgca ggtgaagatc accgccggcc ccatgccgcc ggcgccgcag
      961 atattgaacg agagctatga ctttcagacg cgcgaggcga tcgtgttcac gttcgatcag
     1021 gacgtcagcg cattcctgtc gcgcaaggat tatctgctgc aaaacctgac gacggcgcag
     1081 accgtgccgt cgtccgccgg cacgctgagc tacaacctga acagcaacca ggcgacgctg
     1141 acgctgacca accagctgcc cgacggcaac tataagttga cggtggccgc atccgacatc
     1201 gccaacgccg ccggcgaacc ggcgtcgggc gcgccgatca tcctgaactt cttcgtcctc
     1261 ggcggcgacg ccacccgcga cagcaaagtt gacgtcgggg acctgggcgt gctcgcgacc
     1321 aactatggtt tgagcgggaa gacctactcc caaggcgact tcaattacga cacgaaggta
     1381 gacgtcgggg acttgggcat cctcgccaca aactacggga agagcctcgc cgccggcagc
     1441 gtgttcagcc aaatcgcggt gtcatcggtg gcaaccgcca catcgtcgag acggaccgcc
     1501 gctgcggaac ccgccgccgc cgcgtcggtc tttgcgacgg ttccgctcac ggatgccgcg
     1561 tgggatgccg cgggtcgcag acacgaacga ctgaaggatg tgggactggc gtaagggccg
     1621 atccgctcgc agggcatgcg cctttcgcgc catgcaatgc cccgaggcgc gaatcggatt
     1681 gcaccttcca tgccagcgcc acccaccgat cctctgcatc attcgatgcg gtatttttcg
     1741 atcacggcca cctgcggaca cgtcagcctg ctggcggggc taaggacgat tccagtctgg
     1801 caatcgcctt tttttgtttt accgcaagct cggctgacgg tagatttgtg ctcgatcttc
     1861 gagccagctt aacatttcga ttttgcgaat cgtgcgaatc catctatgga ggggaccttg
     1921 tcgaggcacc tgcgagttcc gcgaaagcgg cctcacaaat ccgttcttag cgcggctgtc
     1981 atcaacaccg ccgagtggct tgagccgcgg cgattgctca gcagcgcgcc agtaggtcca
     2041 gagatcccaa ttgctgccag cagcggcggt acttccctaa cgggaccggc ggttgcgatc
     2101 gacaacgcgg gcgacttcat cgttacatgg tccgccagcg gtggtggttc gaatgggctg
     2161 gatatctggg ctcgccgcta cgatgcggcg ggcgatccgc tgggggatgc ctttgtcgcc
     2221 aacacaacca ccgcgggaga ccaggttgcc ccagcaatcg cgacggatgc cggcggcgat
     2281 ttcgtcatct cttgggcaac catctttgga aacccgagca acgagctttg gacgttatcc
     2341 agccaatcat tcgactccac aggcgcagcg cgcggcggac aggtcacgct gagcaacagc
     2401 ggctggcttc agccgggagc gaaggtgacg atgttttccg acgcatcgtt cgtcgccgcg
     2461 tggcagacga agatacccgg caacaccaat gaggttgtct atcaggacta ttcatccagc
     2521 ggtgtgccca gtggcagtca gatcgtggta acgtcgttaa ccgcctcgat cgtagtcggc
     2581 gtagtgaacg atgcgagcgg ggacatcgca gtcgcctggc tgaatgcaaa tactgccttt
     2641 catctaagcg cctacaacgg gaccggcact gcgctctccg gtgacgtgct ggtcgcatcg
     2701 gcaaacatca tcactgacgc cggcgtcgcg atgacttcca cggggaacat cggcgtcacc
     2761 tggctctcat cgagcagttc gtcagtctat caatgctttg ccaacggctt ctcgctgggc
     2821 ggggtcgcgc agggcgcaaa cat
//
LOCUS       DATIMH010001064         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5168067, whole genome shotgun sequence.
ACCESSION   DATIMH010001064 DATIMH010000000
VERSION     DATIMH010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5168067"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VLI90_18435"
     CDS             complement(<1..1292)
                     /locus_tag="VLI90_18435"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSV16247.1"
                     /translation="MNTKKNNRPAPRVARTVGMLEWFRPGEHDRVDRVLADLHALGVT
                     ELRTGVSWADWHTAEGEAWYAWLFPQLARQVNLLPCFLYTPPSWGVAPKTSAPPRDPR
                     SYADFIDLMIRRFGKHFEYLELWNEPNNLRQWDVALDPYWFTFSEMVGAAAYWAKQCG
                     KKTVLGGLSPVEPGWLRLMGDRNLLQYIDAVGIHAFPGSSEAKWEGWSPIVQGVREVL
                     DQFQPSAEVWITETGYSTWRHDERRQLTAFVDATDAASVDRVYWYAVHDLDPKLPTVD
                     GFHADEREYHYGLRRTDGGEKLLFRLWAEGGIDAVRDAYWIGKSAHVSGRDKPVLITG
                     GCGFIGTNVAHRIMSSGQSVLLFDNLSRPGVEKNLRWLRQEHGDLMRIEAADVQDPHV
                     LRHAVDRADRIFHYAAQVAVTTSLTNAIHDFEVNARGTL"
     gene            complement(1470..2588)
                     /locus_tag="VLI90_18440"
     CDS             complement(1470..2588)
                     /locus_tag="VLI90_18440"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSV16248.1"
                     /translation="MSKRILITGGAGFIGSHLADELLQHGYRVRALDNLSPQVHGPNA
                     RRPDYLSRDLELIVGDVRDKETVRRSLAGVDGVYHFAAAVGVGQSMYEIANYTSLNNL
                     GTAVLLEALIERPVQRLIVASSMSIYGEGLYQDPDGDISTGLERSIEQLRNHEWEVRN
                     NRAQVLTPVPTPESKQPTLASIYALNKFDQERMCLMIGRAYAIPAVAMRFFNVYGPRQ
                     ALSNPYTGVLAIFASRLMNDKPPMIFEDGLQQRDFVSVHDITTACRLALEVPDAANRV
                     FNVGSGHAYTVREIAHKIAKVLDKDDLEPEITGKYRVGDIRHCFADIRLARRLLGYKP
                     RVALQDGLTELAEWLEGQEANDQVAQAQEELSKRGLAL"
BASE COUNT          491 a          917 c          958 g          559 t
ORIGIN      
        1 agcgtgccgc gcgcgttcac ttcgaaatcg tggatggcgt tggtcaggct cgtcgtcacc
       61 gccacctggg cggcgtaatg gaagatccga tcggcgcgat cgaccgcgtg ccgcagcacg
      121 tgcgggtcct ggacgtcggc ggcttcgatg cgcatcaggt cgccgtgctc ctgccgcaac
      181 cagcggagat ttttctcgac acccgggcgc gacaggttgt cgaacagcag cacgctttgg
      241 ccgctggaca tgatgcgatg ggcgacgttg gtgccgatga acccgcagcc accggtgatc
      301 agcacgggct tgtcgcggcc gctgacgtgc gcgctcttgc cgatccaata cgcgtcgcgc
      361 accgcgtcga tgccgccctc ggcccacagg cggaacagca gcttttcccc gccgtcggtg
      421 cggcgcaggc cgtagtgata ctcgcgctcg tcggcgtgga agccatccac cgtcggcagc
      481 ttcgggtcca gatcgtgaac ggcgtaccaa tagacgcgat cgaccgacgc cgcgtccgtg
      541 gcatcgacaa acgcggtcag ctgccggcgt tcgtcatgcc gccaggtgct gtagccggtc
      601 tcggtgatcc acacttcggc gctgggttgg aattgatcga gcacctcgcg cacgccctgc
      661 acgatgggcg accagccttc ccacttcgcc tctgaactgc cggggaacgc gtggatgccg
      721 acggcatcga tgtactgcag caggttgcgg tcgcccatca gtcgcagcca gcccggttcg
      781 actgggctca atccgccgag gacggttttc ttaccacact gcttggccca atacgcggcg
      841 gcgccgacca tctcgctgaa ggtgaaccaa tatggatcga gcgcgacgtc ccattgccgc
      901 aggttgttcg gctcgttcca cagctcgaga tattcgaagt gcttgccgaa gcggcggatc
      961 atcaggtcaa tgaagtcggc gtaactgcgc ggatcgcgcg gcggggcgct ggtcttgggc
     1021 gcgacgcccc aactcggcgg cgtgtacagg aagcacggca gcaggttcac ctgccgggcc
     1081 agctgcggga acagccaggc gtaccacgct tcgccctcgg cggtgtgcca gtcggcccac
     1141 gagacgccgg tgcgcagctc ggtcacgcct agcgcatgaa gatccgccag cacgcgatcg
     1201 acgcgatcgt gttcgccggg gcgaaaccat tcaagcatgc cgacggtccg cgcgacgcgc
     1261 ggggcggggc gattattctt tttcgtgttc attagacgaa cctcaaacag cacctcgtca
     1321 tcacgcgcgc cttctccctc tcctgcgtac tcgtgggaga gggcaggggt gagggtgatt
     1381 tcgagcacga gtggcgcgct catccattcg aaatcaccct caccctaacc ctctcccacg
     1441 ggtacgtggg cgaggggacc gggcgggagt cacagcgcca atccacgctt gcttagttct
     1501 tcctgcgctt gcgcgacctg gtcgttcgct tcttgccctt cgagccactc ggccagttcg
     1561 gtcaatccat cttgcagcgc gacgcgcggc ttgtacccga gcagccgccg ggccaggcgg
     1621 atgtcggcga agcagtggcg gatgtcaccg acgcggtatt tgccggtgat ttccggctcc
     1681 agatcgtcct tatccagcac cttggcgatc ttgtgcgcga tctcgcgcac ggtataagcg
     1741 tgaccgctgc cgacgttgaa gacgcgattg gcggcgtcgg ggacctccag cgccagccgg
     1801 caggcggtgg tgatgtcgtg cacgctgacg aagtcgcgct gctgcaggcc gtcttcgaaa
     1861 atcatcggcg gcttgtcgtt catcaaacgg ctggcgaaga ttgccagcac gccggtgtag
     1921 gggttactca gtgcttggcg cgggccgtag acgttgaaga agcgcatcgc caccgcgggg
     1981 atcgcatacg cccgcccgat catgagacac attcgttcct ggtcgaattt gttcagcgcg
     2041 taaatgctgg cgagcgtggg ctgcttgctc tcgggcgtcg gcacgggcgt caacacttga
     2101 gcgcggttgt tccgcacttc ccactcgtga ttgcgcaact gctcgatcga gcgctccagg
     2161 cccgtcgaga tgtctccatc cgggtcttga taaagacctt cgccgtagat gctcatgctg
     2221 ctggcgacga tcaaccgctg cacgggccgc tcgatcagcg cttccagcag caccgcggtg
     2281 ccgaggttgt tcaggctcgt ataattggcg atctcgtaca tgctctgccc gacaccgacg
     2341 gcggcggcga agtgatagac cccgtctacg ccggcgaggg atcgacgcac cgtctccttg
     2401 tcgcgcacgt cgccgacgat gagttcgaga tcgcggctga ggtaatccgg ccggcgggcg
     2461 ttggggccgt gcacttgtgg gctgaggttg tcgagcgcgc gaacgcggta accgtgctgg
     2521 agcagttcat cggccagatg cgacccgatg aatcccgcgc caccggtgat gaggatgcgt
     2581 ttgctcatag gtgagtcgtc ccgttttcga gtgacgcaac cgactcaaca aatgcaatgc
     2641 ccctgaagtg cggcggcggc aatggtgtgc gcgcggcggc ggttcgcgcg cgctgtcatg
     2701 tgcgcgcaat tcacgtgctc gttcataaat gcgacggttc atcgcgatgt tgcgaggcgc
     2761 gacagccgta cacgcttgta cggaaaatcg attggtgtgt cggggaaagg cgtagaggcg
     2821 cgagcacgta acaacgtcgc tcggagtttc cctgatgcgg gtgcgagttg gtggaggcgc
     2881 gcagcacttc cacgccgggt tcggaagtac tccgacgacc cggat
//
LOCUS       DATIMH010001065         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1121175, whole genome shotgun sequence.
ACCESSION   DATIMH010001065 DATIMH010000000
VERSION     DATIMH010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121175"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VLI90_18445"
     CDS             complement(<1..584)
                     /locus_tag="VLI90_18445"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HSV16249.1"
                     /translation="MLPVTRHGLRELLIGSVVLALCAIGLGYTWWPAALIVLPVFIWL
                     IAFFRDPDRVVPSEQHAMVSPADGTVSDITEIESEPLLGNVPAVRIGIFLSVFNVHIN
                     RSPCDGKVLDVIYKEGKFINAMRHNEASEDNESNTIVLGDPETGRPIAVVKQIVGLIA
                     RRIVSTAKLGQTVERGERIGMIKFGSRTELTIAG"
     gene            complement(759..>1682)
                     /locus_tag="VLI90_18450"
     CDS             complement(759..>1682)
                     /locus_tag="VLI90_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16250.1"
                     /translation="EPLFVSLAPSTADVENKAAVDGIYSLTCGPFRAQVGPLDSGMDM
                     LTAMALGDMDIAGTPVMAGKVVVMDTRDVNKFADKIRTTVYDARDRGAMAAVPRTKRH
                     VQLSYALFKRFTKTTPANVEGPAMGANPFIGPNPLQPSGDPTPPVTARYHDHASAGSW
                     LLDTGAVASMISRKQAAAVGVKYVANTEGTDNPVLAGVPKEQQFTLSVGGIGGSKKAA
                     GFYLDQLSLRTREGQPLNFLHAPVLVTDITVKDQATGQTLTLDGVFGMNFLVASANVQ
                     QAGLMPDIGKLTPGAFRWIVFDQPAGVLGLE"
BASE COUNT          314 a          558 c          494 g          316 t
ORIGIN      
        1 caccccgcga tcgtcagttc ggtccggctg ccgaatttga tcatgccgat gcgctcgccg
       61 cgctcgacgg tctgcccaag cttcgcggtc gagacgatcc gccgcgcgat cagccccacg
      121 atctgcttga ccaccgcgat cggccggccg gtctcggggt cacccagtac aatcgtgttc
      181 gactcgttgt cttccgatgc ctcgttgtgc cgcatcgcgt tgatgaattt gccttcctta
      241 tagatcacat cgagcacctt gccatcgcac gggctgcggt tgatatggac gttgaagacc
      301 gacaggaaga tgccgatgcg cacggccggc acgttgccga gcagcggttc actttcgatt
      361 tcggtgatat ccgaaacggt cccgtcggcg gggctgacca tcgcgtgctg ttcgctgggg
      421 acgacgcgat ccggatcacg gaagaacgcg ataagccaga tgaaaacggg caaaacgatc
      481 agcgcggccg gccaccaggt gtagcccagg ccgatggcac agagggcgag cacaacgctg
      541 ccgatcagca gctcgcgcaa gccgtgacgg gtcacgggaa gcatcagcgc aggctagaac
      601 agcccaacaa ccttgtcaac gtcgccatca agcggtcaaa ctaagaggtt ggaaccacag
      661 atgcacacag atgaacacag atgaacacag atgaacacag ataagaaatg aattgccttt
      721 atctgtgggc atctgtgtgc atctgtggtt gatccttctt attccagtcc aagcacccca
      781 gccggttgat cgaaaacgat ccaccgaaat gcccccggcg tcagcttgcc gatgtccggc
      841 atcaaccccg cctgctgtac gttggcgctg gcgaccagga agttcatgcc gaacacgccg
      901 tccagcgtca gcgtctgccc ggtcgcctgg tccttgaccg tgatgtccgt caccagcacc
      961 ggcgcgtgca ggaaattcag cggctggcct tcgcgcgtgc gcagcgacag ttgatcgaga
     1021 taaaaccccg ccgccttttt cgacccgccg attccgccga ccgacagcgt gaactgctgc
     1081 tcctttggca cgcccgccag caccgggttg tccgtcccct cagtgttggc gacatacttc
     1141 acccccacgg cggcggcctg cttgcgcgag atcatcgacg ccaccgcgcc cgtgtcgagc
     1201 agccagctgc ccgccgacgc gtgatcgtga taccgcgccg tcaccggcgg ggttggatca
     1261 ccgctcggtt gcagcggatt gggaccgatg aacggattgg cgcccatggc cggtccttcc
     1321 acgttggccg gcgtggtctt tgtgaatcgc ttgaacaacg catagctcag ctgcacgtgc
     1381 cgcttggtcc gcggcaccgc cgccatcgcc ccgcggtcgc gggcgtcgta aacggttgtc
     1441 ctgattttgt cggcgaattt gttgacgtcg cgcgtgtcca tcacgacgac cttgcccgcc
     1501 atcaccggcg tgccggcgat gtccatgtcg cccagcgcca tcgcggtcag catgtccatc
     1561 ccgctgtcca gcgggccaac ctgcgcacgg aaaggtccac acgtcaggct atagatgccg
     1621 tccaccgcgg ccttgttctc gacgtccgcc gtcgatggcg cgagcgacac gaacagcggc
     1681 tc
//
LOCUS       DATIMH010001066         1905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_833786, whole genome shotgun sequence.
ACCESSION   DATIMH010001066 DATIMH010000000
VERSION     DATIMH010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833786"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..822
                     /locus_tag="VLI90_18455"
     CDS             82..822
                     /locus_tag="VLI90_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16251.1"
                     /translation="MIFLLCIGLPFFVICLIDSLTTKVQPNWPAPAYFTLLILAAYFI
                     TTRAETIDRWRRWRRWVYGAIVFGVLVQPLARGSTIVYTTAAWANRVFPHAKLTPQKF
                     DPAFKLRGVADPFARAITEHLKQLPPGSFILCEDYQDASQLAFYVDGQPNTYFAGSYW
                     TDPAVRRRRTQFDMWPDRQLDRPELIGREVIYVGTMAFAPLKESFERVEPLPKIVVER
                     NGFELRSYQPWRCYGFKGMHRPAGQGSY"
     gene            963..1577
                     /locus_tag="VLI90_18460"
     CDS             963..1577
                     /locus_tag="VLI90_18460"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSV16252.1"
                     /translation="MILAIAWLALFVVALPLDRSVAVAVHNAGIDARLRGSMLAEIVK
                     TPGTFYFAIAIAILGLLTRQVRRGEGVFVLLASAMSGINGLIKWLVGRTRPFKLTGPD
                     QPQPFHFEPLWHGVNGLFHQKDLCFPSGHACTAFALATAVALIRPRWSPVFFAIALLV
                     AAERVLENAHYLSDTVAAAGVGAGGTLFVWWLMCRFLKPPLLLS"
     gene            1574..>1905
                     /locus_tag="VLI90_18465"
     CDS             1574..>1905
                     /locus_tag="VLI90_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16253.1"
                     /translation="MKIFLDTVLIVAVIESILTFLASFGHRLGKLGRSVTDACTRAPL
                     IDLVLGVFTWIPWLIAGVLFGWRGFFGALVGEFIAYHFWVISHELIHRDAVRGPRIVK
                     FINRTIGR"
BASE COUNT          298 a          624 c          584 g          399 t
ORIGIN      
        1 gacccggatt cttctgcgag agagaaagca tccgggtcgt cggagtacct ccgaacccgg
       61 cgtgatccat atcggcgcga gatgattttc ctcctctgta tcggcctgcc gttcttcgtc
      121 atctgcctga tcgattcgct cacgacgaag gtgcagccta actggcccgc gccggcgtat
      181 ttcacgctgc tgatcctggc cgcgtacttt attacgacgc gagcagaaac catcgatcgc
      241 tggcgccgct ggcgccgctg ggtgtacggc gcgatcgtct tcggcgtcct ggtgcaacca
      301 ttggcgcgcg gttccacgat cgtctacacc accgccgcgt gggcgaaccg cgtgtttccg
      361 cacgcgaagc tcacgccgca gaagttcgat ccggcgttca agctgcgcgg cgtcgctgat
      421 ccattcgcgc gggcgatcac cgagcacctg aagcaactgc cgccgggatc gtttatcctt
      481 tgcgaggact accaggacgc gtcgcagctg gcgttttacg tggacggcca gccgaacacg
      541 tacttcgccg gttcatactg gaccgacccg gcagtccgcc ggcggcggac gcagttcgac
      601 atgtggcccg accggcagct cgatcggccg gaattgatcg gccgcgaggt gatctacgtc
      661 ggcacgatgg cgttcgcgcc gctgaaggaa tccttcgagc gcgtcgaacc gctgccgaag
      721 atcgtggtcg agcgaaacgg gttcgagctt cggtcgtatc agccgtggcg atgttacggg
      781 ttcaaaggca tgcatcgacc ggcggggcaa ggttcgtatt gaacgtcttc cccgtgacac
      841 ggggctccgg cacgtgctgg gtgcaaatgg atttagacaa atccacgatt actgatgttt
      901 cagaaccgct ggcacgggcc ggaggcccgt gtcacggggg gcattcttat gcgcgtcgat
      961 ggatgatcct cgcgatcgct tggctggcgc tgttcgttgt cgcgctgccg ctcgatcgat
     1021 cggtggccgt cgcggtgcac aatgccggca tcgatgcgag gctccgcggc agcatgctcg
     1081 ccgaaatcgt gaagacgcca gggacgttct acttcgccat cgccatcgca attctcggat
     1141 tgctcacgcg gcaagttcga cgcggcgaag gggtcttcgt cctcctcgcc agcgcgatga
     1201 gcggcatcaa cggcttgatc aaatggctcg tcggccgcac gcgaccgttc aaattgaccg
     1261 ggccggatca accgcagccg tttcacttcg agccgctgtg gcacggcgtc aatgggctgt
     1321 ttcaccagaa ggacctgtgc ttcccatccg gccacgcgtg caccgcgttt gcgctggcca
     1381 ccgcagtcgc gctgatccgc ccgcggtggt cgccggtgtt tttcgcgatc gcgctgctcg
     1441 tcgccgccga gcgtgtgttg gagaacgcgc attacctcag cgacaccgtc gccgccgccg
     1501 gtgtcggcgc gggcggcacg ctcttcgtct ggtggctcat gtgccgcttt cttaaaccgc
     1561 ccctcttgct gtcatgaaaa tttttctcga caccgtcctc atcgtcgccg tcatcgaatc
     1621 gatcctgacc ttcctcgctt ccttcggcca ccgcctcggc aagcttggtc gatccgtcac
     1681 cgatgcctgc acgcgcgcgc cgctgatcga cctcgtcctc ggcgtcttca cgtggatccc
     1741 ctggctgatc gccggcgtcc tcttcggctg gcgcggattc ttcggcgcgc tagtcggcga
     1801 attcatcgcg taccacttct gggtgatcag ccacgagctg attcatcgcg acgccgtacg
     1861 cggcccgcgc atcgtgaagt tcatcaaccg cacgatcggc cggtg
//
LOCUS       DATIMH010001067         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1992141, whole genome shotgun sequence.
ACCESSION   DATIMH010001067 DATIMH010000000
VERSION     DATIMH010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992141"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="VLI90_18470"
     CDS             <1..1079
                     /locus_tag="VLI90_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16254.1"
                     /translation="RSGTPFTPSISGVVNGDGYSNDRAFIFDPAKTQDPALASAMQSL
                     LNNSTGRAKDCLLKQLNDFAGRNSCEGPWTSSANLSISFNPAKVRLPQRATLSFAVSN
                     PLGAADMLLHGSNNLHGWGQFSFTDQTLYYVRGFDADAKQYRYEVNPRFGNSNPQFNA
                     FRAPVTLTMQLRIDVGPTRERQQLTQLLDRGRKTEGQKVPEQLLRAQYGSGGILNPMS
                     QMLRQMDTLGLSGMQADSLASMNRRYTIRLDSIWSPVTKYLADLPEHYDQAEAYDRYT
                     RARKASVDMLIHLAPTINALLTDEQKRKLPALVASYLDPSYLAGIRSGTAGGNGGGAF
                     GGFGGGGFGGGGGGGGGRGGRGGG"
     gene            994..>2191
                     /locus_tag="VLI90_18475"
     CDS             994..>2191
                     /locus_tag="VLI90_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16255.1"
                     /translation="MARSVDSVAAASVVAVAAAVDVAGVAAVDLFFRAVQLIETHMIQ
                     HSFTVARAGTLLVLATLGATTVQAQQASQEQQLQPSSAQQASARPAVRPLGAALATSE
                     PLGALSAVRQLPGGKLLVNDPAKRRVLLLDSTMKTISIVADTTPATQNAYGTRGGGIL
                     AFRGDSTLFLDPASLSMLVIDPNGAVTRVMAAPRPDDVNSLVGGALGFPGFDSKGRLV
                     YRAGFNFNRRPGGAGNGGGFQPPVLPDSVPLVRFDLATRKLDTAAYIKVASPKINFSE
                     NSDGRRQVSIVINPMPEVDDWAVLSDGTIAILRKDYHVDYINADGTKSSSPRIPFDWQ
                     RLNDSAKSAVIDTARAQMERVRAGGPAALQTLASGDGGFGGFGGGGFAGGGFGGGFGG
                     GGFGGGG"
BASE COUNT          378 a          702 c          730 g          381 t
ORIGIN      
        1 ttcgcagtgg cacgccgttc acgccgagca tctccggtgt tgtaaatggc gacggttact
       61 cgaacgaccg cgccttcatc ttcgatccgg ccaagacgca agaccccgcg ctcgcgagcg
      121 cgatgcaatc gctgctgaac aacagcacgg ggcgcgcaaa ggactgtctg ctcaagcagc
      181 tgaacgactt cgcgggccgc aactcgtgcg agggtccgtg gacgtcgagc gcgaacctca
      241 gcatctcgtt caatccggcc aaggtgcgct tgccgcagcg cgccacgctc agcttcgccg
      301 tctccaaccc actcggcgcg gccgacatgc tgctgcacgg ctcgaacaac ctgcatggct
      361 ggggtcagtt cagcttcacc gatcagacac tgtactacgt ccgtggattc gacgccgacg
      421 ccaagcaata ccggtacgag gtgaacccgc gcttcggcaa cagcaatccg cagttcaacg
      481 cgttccgggc gccagtgacg ctcacgatgc agctgcgcat cgacgtgggt cccactcgcg
      541 agcggcagca gctgacgcag cttctggatc gcggtcggaa gacggaaggg cagaaggtgc
      601 cggagcagtt gctgcgcgcg cagtacggca gcggcggcat cctcaatccg atgtcgcaga
      661 tgctccggca aatggatacg ctcggcttga gcggcatgca ggcggatagt ctggcgagca
      721 tgaatcgccg ctacaccatc cggctggaca gtatctggag tccggtcacg aaatatctgg
      781 ccgacctgcc agagcactac gatcaggcgg aggcgtacga tcggtatacg cgtgcacgga
      841 aggcgtcggt ggacatgctc atccacctcg cgccaacgat caacgcgctg ctcaccgatg
      901 agcagaagcg gaaactgccg gcgctcgtcg ccagctatct cgacccgagc tatctcgccg
      961 gcattcgcag cggtaccgcg ggcggcaacg gcggtggcgc gttcggtgga ttcggtggcg
     1021 gcggcttcgg tggtggcggt ggcggcggcg gtggacgtgg cgggcgtggc ggcggttgat
     1081 ctttttttcc gagcggttca actcatcgag actcacatga ttcagcattc tttcacggtc
     1141 gcgcgcgccg ggacgctgct cgttctcgcc acgctcggtg ccacgaccgt tcaggcgcag
     1201 caggcgtcac aagagcagca gctccaacca tcatcggcgc agcaagcgtc ggcgcggccg
     1261 gcggtgcgac cgctgggcgc ggcgctcgcc acgtcggagc cgttgggtgc gttgagcgcg
     1321 gtgcgccagc tgccgggtgg caaactgctc gtgaacgatc cggcgaagcg gcgggtgctg
     1381 ctgctcgact cgacgatgaa gacgatctcc atcgtcgccg acacgactcc cgcgacgcag
     1441 aacgcctacg gcacgcgcgg cggcggcatc ctggcatttc gcggcgactc cacgctgttc
     1501 ctcgatcccg catcgctctc gatgctcgtg atcgatccga acggcgccgt cacgcgcgtg
     1561 atggcagcgc cgcgtcccga cgacgtgaac tcgctcgtcg gcggtgcgct cggctttccc
     1621 ggattcgatt cgaaaggccg gctcgtgtat cgcgccggat tcaacttcaa tcggcgtccc
     1681 ggcggagcgg ggaatggcgg cggcttccag ccgcccgtcc tgcccgacag tgtgccgctc
     1741 gtgcgcttcg atctcgcgac gcgcaagctc gacacggcgg cgtacatcaa ggtggcatcg
     1801 ccgaagatca atttctccga gaacagcgat ggacgccgcc aggtatccat cgtgatcaac
     1861 ccgatgccgg aggtcgatga ttgggctgtg ttgtccgacg gcaccatcgc gattctccgc
     1921 aaggactatc acgtcgacta catcaatgcc gacggcacga agtcgtcgtc gccgagaatt
     1981 ccgtttgatt ggcagcggct caacgactcc gcgaagagcg cggtgatcga taccgcgcgc
     2041 gcgcagatgg aacgcgtgcg cgctggcgga ccagccgcgt tgcaaacact cgcctcgggt
     2101 gacggcggct tcggtggctt cggcggaggc ggcttcgctg gcggtggatt cggcggtggg
     2161 ttcggcggcg gaggattcgg tggtggtggt t
//
LOCUS       DATIMH010001068         4640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4878264, whole genome shotgun sequence.
ACCESSION   DATIMH010001068 DATIMH010000000
VERSION     DATIMH010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4878264"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(631..2862)
                     /locus_tag="VLI90_18480"
     CDS             complement(631..2862)
                     /locus_tag="VLI90_18480"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSV16256.1"
                     /translation="MHSDNSSSSNKPAALAMLASERPRNVGWVQAAGLLFGDWGTSRL
                     YVLGLAFLFAGRTSFYLICLMSVLILAVGWAYTQICRIYPDGGGVYTAAKHKSRTLAV
                     IGALLLFADYTVTASLSVLDAFHYFGLPLQKHAQVAESTVAANSGSQNRIADAGDNII
                     QESDTAQTVDLSALPPGVSIPSDIKEVRFDADKKQLRVDGQMSALTRGDLLALSTDPA
                     WQHAVRKLYDESQPEQFLAWDSPGLWAMIAIVGIGLFNLMGPKHTGGFAIFAAVGMVF
                     ITLLITCFALMSGKVDWHNIHFGTLHHPPGEMWVAFVSIVLALSGVEAIANLTGVMKK
                     PVAHTARKAIWVVALEVACFNIILALFMMAIFPLDRQAHVNDMLAFLTHFYVGPWGAW
                     PVRIVGGLLLLSAGNTAITDMISVQYLMARDGELPQALVKLNRFGVPWVPALIAASVP
                     VLVLLVSHDLEALAALYAIGVVGAVAINVTLCSTHPRLRRMHRKLPMMLLGVVLLAIW
                     VTLACTKLHALIFVTIVMVFGLSARAATKWFSTRKGPRVSLLRQAIMQQLSEESFVKP
                     KILLGTYGSDVMAATALRIAKERNATLVVCFIRQVTLSYKYEAKERMTIDTDLAALRL
                     FAKILDMAHAMNVSVIPHYDTGPDAAELMAETAAMMGCERVLIGTSRQGAIYHLFKGT
                     FQRRLEDILPPDIPVEVISPPPPAPADAQAQSPQQPPTAPPPTDPPVAKAEEADEHVP
                     VGR"
     regulatory      complement(3035..3193)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(3305..4063)
                     /locus_tag="VLI90_18485"
     CDS             complement(3305..4063)
                     /locus_tag="VLI90_18485"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HSV16257.1"
                     /translation="MSFCQLHWNSQTLQKQTTTWVLLPEVGTPPFAVFYLLHGLSDDH
                     TMWMRRTRIEAYADRWPIIIVMPDGGRGFYTNHVAGPKWATHIAEELPARIERTFGAK
                     TTRDGRCIGGLSMGGYGAMRLALGYPDRYVSANSHSGALLGPSMHRGVISMDEYHQMF
                     GAAYEGSDSDLLVLAKRAKTSGVTLPKLMIDCGRDDHLLEANRTVHRELAALQIEHAY
                     DDDLPGGHDWNYWDARISTAIEFHARHLCVRPAS"
     gene            4121..>4640
                     /locus_tag="VLI90_18490"
     CDS             4121..>4640
                     /locus_tag="VLI90_18490"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV16258.1"
                     /translation="MTTATETPLMTTAELLALPDDDGIERELVRGRLKDREMTRRNRR
                     HARSGAKLAKMLGVWLEQQPEPRGEILVGDAAIRLRQNPDSTVAVDLAYISPQMARAN
                     PDDAFVLDGVPILMVEILSPSDKQEDILDKVRDYLDAGVPLVWIAEPVFRTVTVYQPN
                     AEPVLYTVKQTIT"
BASE COUNT          875 a         1415 c         1487 g          863 t
ORIGIN      
        1 catgcacaag aaatgccatg ccattgtcca cgaaatcgac ccggtcagag tcaatgcagc
       61 tatctaatgg cgtcacctgc gcgagccaag tggtgcagtt cggtagaacg gactatcaag
      121 gacggaaaaa acaggtagct cggacagctc tggggtgaga aaaaagggac gcacaaaatt
      181 gttaattcag atcaatcgtt gcccgttacg ctagcgcctt tatccggccg aataccttcg
      241 cgtcacttcg gcgtcacgcg gttcatcgtc gcggcgggca tggcgtcggc gacgccgcgg
      301 acgcggcggg tctggcagct tcttcgaacg tcagctcttc gagcttgctc gggcgggcgt
      361 tgggctgctc gtcgcggcgt tcgtccgtgg ccggccgctc cggcggctgg gcgtgatgct
      421 gccgatcctc gtgccagata aatgttgata tgggtagtct ggtgaagatg gagataaaat
      481 cgagcgccgc gcaatacctc gctcgctttt tgacgcggaa gcaagaacgt atcttcagta
      541 ttcacagtcg ctaacgcgca gaaaagccgg cgcaacatcg cgaaaatatt ctccttgcgc
      601 gccaggtccc agccggtcga tcggcaaccg ctaccggccc accggcacgt gctcgtccgc
      661 ttcttccgcc ttggcgaccg gcgggtcggt gggtggcggg gccgtcggag gttgctgcgg
      721 ggattgggct tgcgcgtccg ccggggcggg gggtgggggg ctgatcactt ccaccggaat
      781 gtccggcggc aggatgtctt ccagccgccg ttggaatgtg cctttgaaca agtgatagat
      841 cgcgccttgt cggctggtgc cgatcaggac gcgttcgcag cccatcatgg cggcggtttc
      901 ggccatcaac tcggcggcgt cggggccggt gtcatagtgc gggatgaccg acacgttcat
      961 cgcgtgggcc atgtccaaga tcttggcgaa caggcgaagg gcggccaagt cggtgtcgat
     1021 cgtcatccgc tccttagctt cgtatttgta cgacagcgtg acttggcgga tgaagcagac
     1081 gacgagcgtg gcgttgcgtt ccttggcgat gcgaagggcg gtggcggcca tcacgtcgga
     1141 gccataggtg ccgagcagga tttttggctt cacaaaagac tcttcgctca actgctgcat
     1201 gatggcctgc cgcagcaggc tgacgcgcgg gcccttgcgg gtgctgaacc acttggtggc
     1261 agcgcgggcg ctgaggccga agaccatgac gatggtgacg aagatgagcg cgtgcagttt
     1321 ggtgcaggcg agggtgaccc agatggcgag caggacgacg ccgaggagca tcatcggcag
     1381 cttgcgatgc atgcggcgaa gccgcgggtg ggtcgagcag agcgtgacgt tgatggcgac
     1441 ggcgccgacg acgccgatcg catacagcgc ggcgagcgct tccagatcat gactgaccag
     1501 cagcaccagc accggcacgc tggcggcgat gagcgccggc acccagggca cgccgaagcg
     1561 gttcagcttc accagcgctt ggggcaactc gccatcgcgg gccatcaggt actgcacgga
     1621 gatcatgtcg gtgatggcgg tgttgccggc ggatagcagc agcagaccgc cgacgatgcg
     1681 caccggccac gcgccccatg ggcccacgta aaagtgcgtg aggaacgcca gcatgtcgtt
     1741 cacgtgagct tgtcgatcga gcggaaagat cgccatcatg aacagcgcga ggatgatgtt
     1801 gaagcaggcg acttccagcg cgaccaccca gatcgctttt cgcgcggtgt gggcgacggg
     1861 ttttttcatt acgccggtca ggttggcgat cgcctcgacg cccgacaggg cgagcacgat
     1921 cgatacgaac gccacccaca tctcccccgg cgggtgatgc agcgtgccga agtggatgtt
     1981 gtgccagtcc accttgccgc tcatcagcgc aaagcacgtg atcagcagcg tgatgaacac
     2041 catgccgacg gcggcgaaga tcgcgaaccc gccggtgtgc ttcgggccca ttaggttgaa
     2101 caaaccgatg ccgacgatgg cgatcatggc ccacaggccg ggcgagtccc atgcgaggaa
     2161 ttgttccggt tgcgattcat cgtacagctt gcgaacggcg tgctgccaag cggggtcggt
     2221 ggacagcgcg agcaagtcgc cgcgggtgag cgcggacatc tgtccgtcta cgcgcagctg
     2281 ctttttgtcc gcatcgaagc gaacctcttt gatgtccgat ggaatgctca cgccgggcgg
     2341 cagggcggat aagtcgacgg tttgggcggt atcgctttcc tggatgatat tatcgccggc
     2401 atcggcaatt cggttttgac tcccgctgtt ggcggctacg gtgctctcgg ccacctgcgc
     2461 gtgcttctgc agcggcaggc cgaagtagtg gaacgcgtcg agcacgctca agctggccgt
     2521 cacggtgtag tccgcgaaca gcagcagcgc cccgatgacg gccagcgtgc ggctcttatg
     2581 cttcgccgcc gtgtagacgc cgccaccatc ggggtagatc cggcagatct gcgtgtacgc
     2641 ccaacccacc gccagaatga gcacgctcat caggcagatc aggtagaagc tggtgcgacc
     2701 ggcgaagaga aacgccaggc ccagcacata caaccggctg gtgccccaat cgccaaacaa
     2761 caatccggcg gcctgcaccc accccacatt gcgcgggcgt tcgctggcca acatcgccag
     2821 ggcggcgggc ttgttactac tactgctgtt atcgctatgc atatccgatc atcccaatgg
     2881 acgaccgccg tgctgcgcta agatcgcaaa cactgcgcgc gtgcgcacgc aggcgacatt
     2941 gcgaaagcaa ttgcaaaggc acaattgcac agcgtttggc tcaaacggaa atcagcgtgg
     3001 ccaggaaagc cacgctgggt tgagcgcgag acgttgtgcc cgtttccatt gcctgcgggg
     3061 ttagctgacg ggctagggct ggagacgtgc cctgaggcca ggttggttat tctggtctct
     3121 acgccccgtg cttcggtcgt ttctgatcga agcctttggg ttccccgcac caaaccggtg
     3181 gcccggttcg atctcggcgg tcacatggcg gtaaaggcta agatgcgcga gggatgatgt
     3241 caaacgggca ttttgccggt ggttttagcc atttcgcaag catcgctgca atgaggaatt
     3301 acgttcaaga cgccggccgg acacacaggt gtctcgcgtg gaactcgatc gctgtcgaga
     3361 tgcgcgcgtc ccagtagttc caatcatgcc cgccgggcag gtcgtcgtcg tacgcgtgct
     3421 cgatctgcag cgccgctagc tcgcgatgga ccgtgcgatt cgcctccagc agatgatcgt
     3481 cccggccgca gtcgatcatc agcttcggca gcgtcacgcc gctcgtcttc gcgcgctttg
     3541 cgaggacgag cagatcgctg tcggaccctt cgtacgccgc gccgaacatc tggtgatact
     3601 cgtccatcga gatgacgccg cggtgcatgc tcgggccgag cagcgcgccg ctgtggctgt
     3661 tggccgagac gtagcggtcc ggatagccca gcgccaatcg cattgcccca tacccgccca
     3721 tcgacagccc gccgatgcac cggccatcgc gcgtcgtctt cgccccaaag gttcgctcga
     3781 tccgcgccgg cagttcttcg gcgatgtgcg tcgcccactt cggtcccgcc acgtggttgg
     3841 tataaaaccc gcgcccgccg tccggcatca cgatgatgat cggccagcga tccgcgtacg
     3901 cctcgatgcg cgtccgccgc atccacatcg tgtggtcatc gctcaaccca tggagcaaat
     3961 aaaacaccgc aaacggcggt gtgccgacct cgggcagcaa cacccacgtc gtcgtctgct
     4021 tttgtaatgt ttggctgttc cagtggagtt ggcagaagga catggcgctg agggcttacc
     4081 gcaaatcgcc cggctttgct acaatgattc gggagacacg atgacgacgg ccaccgaaac
     4141 gccgctgatg acgaccgcgg aattgctggc tttgcccgat gacgatggca tcgagcgcga
     4201 gctggtgcgc gggcggctca aggaccgcga gatgacgcgg cgaaatcggc ggcatgcacg
     4261 aagcggcgca aagctggcga agatgcttgg ggtctggttg gaacagcagc ccgagccccg
     4321 cggtgaaatc ctcgtcggcg acgccgccat ccggcttcgt cagaatcccg actcgaccgt
     4381 tgctgtcgat ctggcataca tctccccgca gatggcccgt gccaatcccg atgatgcgtt
     4441 cgtcctggac ggcgtgccca tcctgatggt cgagatcctc tcgcccagcg acaagcagga
     4501 agacatcctc gacaaggtcc gcgactatct cgacgccggc gtgccgctcg tctggatcgc
     4561 cgagccggtc tttcgcaccg tcacggtcta tcaacccaac gccgagcccg tgctctacac
     4621 cgtcaaacaa acaatcaccg
//
LOCUS       DATIMH010001069         4972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1993279, whole genome shotgun sequence.
ACCESSION   DATIMH010001069 DATIMH010000000
VERSION     DATIMH010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993279"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="VLI90_18495"
     CDS             <1..1592
                     /locus_tag="VLI90_18495"
                     /inference="COORDINATES: protein motif:HMM:NF016146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HSV16259.1"
                     /translation="WLNVTSSQVTNTYTGIASDEFELRPANPTINSTRNDNWYGYGSL
                     RGTGANTGYGNVTSGGSTYWYPGDADRGDVARSLFYMATRYGQNQATGTNLTLANGPS
                     PTDGSRYVMGDLASLLKWNYEDGVDNFERRRNQIIYSSALNPTYYQGNRNPYIDHPEY
                     VWSVFGGGNNDSQLYVGSSTPASGGSTANVDLGRVLVNATLGTQSVTLNKSGVDPTTY
                     DITTSGSATTAAAGTGQPFDYNAQARTMTVGLSASTATPGVKSGSVTIDNTDLTTAGA
                     GTGSGDGNDTVNVTATVLAHATASFTTASVNTGVTIDLGTFEQGSGTHSTPFAIANYA
                     SAAGFTAGLDLDSIAGSGDQSALGTNVTIFSALAGGSANAYSATMNTSATGSFAATYR
                     LLGSDENLPGATGGQQLTITLSGNVVDRLPGDANFDGTVNVGDLGVLASSYASAAPGG
                     WTDGDFNGDGIIDVSDLGILATDYGQSITIGGGSMTYGTTGGAAVAGASSNVSVPEPT
                     SLALLALSMPAFAHRRRSRSE"
     gene            1905..4772
                     /locus_tag="VLI90_18500"
     CDS             1905..4772
                     /locus_tag="VLI90_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16260.1"
                     /translation="MSSGQYSGFGIGGSAWPEMTAALNLATNNHVAQTSDFSSLSLMM
                     GYDALWLDLRNTTSALTSTELSNIQSYISTGRHVVMIGENFNWTTWDNSILGLISTSV
                     TQSVTTGTAVPIISHPLTSGVESISIPAGGSAVGGTSLFSQPVAQLGGLSNNVLAVLD
                     VDIFSHSFWGTQSNSTFGADVANWAADAGGGNWINSTGGSWSTGTNWAVANPPGAGDD
                     ALFNLKSANGYTVSLSTNSVANKVIVQTDQLTLALGSSSLTTTSDLRVGDLTGQNGSL
                     TITGSTGSIVTGYDVQVGGDGTGSLTQTGGTLTAGHLMYLGADATGNGTFNLQGGTVN
                     AELYVGFDGTGTFNMSGGTFAEVGNNSLIFGYHAGSHGSGTISGTASVTTGYDVSIGA
                     SGTGTLTQTGGKLVVGHVFYVGNSTGGTGTFVLQSGTASTNGSLNLGASGGTGTVTIT
                     GGALTVGGTTNIANSASKIFLTGGTFSTGSLNSADVYRSFYDNWTGGALNLTASGLVI
                     SIIGSLEDAVVLDGTGMALSTSGDIKLGAGPNFGSLLLKNGASVTALGSSSIVIGAGD
                     NNPGNSNATIGNGTLTAGYDLDIGLGARGTITQTDGVTTVGHSLNVGINGGTGTYNIK
                     QNATVIAPTLYLASGSFSFGIVNISDAASLTVADVSKVFNDNSKINLSGGTFKTGSLD
                     LGGVSSRLNWTGGTLWLTGGTITSGTTPLSVGGTFKGTGTVSGATSILAGGRVSPGDG
                     VGTLHLADTTFAAASSLDLELGGSGAGQFDVLDVAGSVALAGPSLNVSLINGYTPPIG
                     SSFTVITNDGSDAITGTFAGVNQGSALRVDRNGHPNFFRVSYIGGTGNDVTLTTIEMP
                     GDANSDQKVDVGDLGVLATNYNTTAGATWAMGDFDGNGRVDVADLGALATYYGQSSAD
                     SPAPALALSSTSAVPEPAATGLAAMVVVGFGRRMRGRAGRR"
BASE COUNT          976 a         1593 c         1548 g          855 t
ORIGIN      
        1 attggctcaa cgtgaccagc agccaggtca ccaacaccta caccggcatc gccagcgatg
       61 agtttgaact tcgcccggcc aacccgacga tcaactcgac gcgcaacgac aactggtacg
      121 gctacggcag cctgcgcggc accggcgcga acaccggtta cggcaacgtc acctccggcg
      181 gcagcaccta ctggtatccc ggcgacgccg accgcggcga tgtggcgcgg tcgctctttt
      241 acatggccac gcgatatggc cagaaccaag ccacgggaac caatctcacg ctcgccaacg
      301 gcccgtcgcc gaccgatggc tcgcgctatg tgatgggcga cctcgcgtcg ctgctgaagt
      361 ggaactatga agacggcgtc gacaacttcg aacgccggcg aaaccagatc atctatagtt
      421 ccgcgctgaa cccgacgtac taccagggca atcgcaatcc atacatcgat caccccgagt
      481 acgtgtggag cgtcttcggc ggcgggaaca acgactcgca gctgtacgtc ggcagcagca
      541 ccccggccag cggtggttcg acggccaacg tcgatcttgg ccgcgtgctg gtgaacgcga
      601 cgctgggcac gcagagcgtg acgctgaata aatccggcgt cgatccgaca acgtacgaca
      661 tcaccaccag cggcagcgcg acgactgccg ccgcgggcac ggggcaaccg tttgattaca
      721 acgcgcaggc gcgcacgatg acggtgggac tgtcggcatc caccgccacg ccgggcgtga
      781 agagcggctc ggtcacgatc gacaacacag acctgaccac cgccggcgcc ggcaccggat
      841 cgggcgacgg caacgacacg gtaaatgtca ccgccaccgt gctggcgcac gcgacggcgt
      901 cgtttacgac ggcgagcgtg aacaccggcg tgacgatcga cctgggcacg ttcgagcaag
      961 gcagcggcac gcacagcacc ccgttcgcga ttgccaacta cgccagcgcc gccggcttca
     1021 ccgcggggct ggatttggac agcatcgccg gcagcggcga ccagtcggcg ctgggcacaa
     1081 acgttacgat cttcagcgcg ctcgctggcg gatccgccaa cgcgtacagc gcgacgatga
     1141 acaccagcgc gaccggctcg ttcgccgcga cataccgatt gctcggctcc gacgaaaatc
     1201 tccccggcgc gaccggcggt cagcagttga cgatcactct gagcggcaac gtcgtcgatc
     1261 gtctgcctgg cgacgcgaac ttcgacggca cggtaaacgt cggcgacctc ggtgtgctgg
     1321 ccagcagcta cgccagcgcc gcccccggcg ggtggaccga cggcgacttc aacggcgacg
     1381 gcatcatcga tgtttcggac ctgggcatcc tcgccaccga ctacggccaa tcaatcacga
     1441 tcggcggcgg gagcatgacc tacggcacca caggcggcgc cgccgtcgcc ggcgcctcgt
     1501 ccaacgtcag cgtgccggaa cccacgtcgc ttgcattgct cgcgctgtcc atgcctgcat
     1561 tcgcgcatcg gcgacgttcg cgcagcgaat agacgggtcc gacgtgaccc cctctcgtgg
     1621 aataatcggc agagtgcggt taagcaaagt ttttcaaata tgaattgttt gatttaatag
     1681 ctatccgctc atcgcgctgc taaattttgc ggggtcggga cgtacgcggt tgcgtccact
     1741 tcttctttgt tcgggcaatc ctaatgaaat cgattcacct tcgggggaac gagactgcgc
     1801 gcttgcgcgc aagatctcgg cgtgcagcgt tggtcatcgc cgcggcagcc gcgggcttgt
     1861 tcccgccatg gagcgctacc acatcgttag ccgggacggt gctcgtgagc tccggtcaat
     1921 attccgggtt tggcatcggc ggttcggctt ggcccgagat gaccgcggcg ctgaatttag
     1981 ccaccaacaa tcacgtggcg cagaccagcg acttcagcag cttatcgcta atgatgggat
     2041 atgatgcgct gtggttagac ttgcgaaaca caacctcggc cttgaccagt acggaactta
     2101 gcaacatcca gtcgtacatt tcgaccggcc gccacgtggt catgatcggg gagaatttta
     2161 actggacgac gtgggacaac tccattttag ggctgatctc cacgagcgtc acgcaatcgg
     2221 tgaccaccgg gacggccgtc ccgatcatca gccatccgct aaccagtggc gtggaatcga
     2281 tatcaatccc tgcaggtgga tcggcggtgg gcggaacgtc gctcttttca cagccagtcg
     2341 cacaattggg tggcttgagc aacaacgtgc tggccgtcct tgatgtagat attttttcgc
     2401 actcgttctg gggcacccag agcaacagta cgttcggagc cgacgtggct aactgggcgg
     2461 ccgacgcggg cggtgggaat tggatcaatt ccaccggcgg cagttggtcg actggcacca
     2521 attgggcggt ggccaatccg cccggcgcag gcgacgatgc ccttttcaac ctcaagtccg
     2581 ccaacggcta cacggtcagc ctttccacca attcggttgc caacaaggtc attgtgcaga
     2641 ccgaccagct tacgctggcg ctgggcagca gcagcctgac caccaccagc gatcttcgcg
     2701 tgggtgacct caccgggcag aacggtagct tgacgatcac cggctcaacg ggatcgatcg
     2761 tcaccggata cgacgtgcaa gtgggcgggg acggcaccgg ctcgttgact caaaccggcg
     2821 gtacgctgac cgcagggcac ctgatgtacc tgggcgccga cgccaccggc aacgggacgt
     2881 tcaacctcca gggcggcacg gtcaacgccg agctttacgt cggcttcgac ggcaccggca
     2941 cgttcaacat gagcggcggc acatttgccg aagtgggcaa caactcgttg atcttcggct
     3001 accacgcggg ctcccacggc agcggcacga tcagcggaac ggcgtcggtc accaccggct
     3061 atgacgtttc catcggcgcg agcggcacgg gcacgctcac gcagaccggc ggcaagctcg
     3121 tcgtcgggca cgtcttctac gttggcaata gcaccggcgg caccggcaca tttgtgctgc
     3181 agagcggcac cgcatccacc aatggaagcc tgaatctggg cgcgtctggc ggaaccggaa
     3241 ccgtcacgat caccggtggc gccttgaccg ttggcggcac aaccaacatc gctaacagcg
     3301 ccagcaagat ttttctcacc ggcggcacgt tctccaccgg ctcgctcaat tccgccgatg
     3361 tttatagaag cttctatgac aactggaccg gtggcgcgct gaacctgacc gccagcgggc
     3421 tggtgatcag tattatcgga tcgctggagg acgccgttgt ccttgacgga accggcatgg
     3481 cgcttagcac cagcggcgat atcaagctcg gcgctgggcc gaatttcggt tcgctgctcc
     3541 tgaagaacgg cgcctcggtc acggcacttg gcagttcgag catcgtcatc ggggccggcg
     3601 acaacaaccc cggcaacagt aatgccacca tcggcaacgg caccctcacc gcgggttatg
     3661 acttggacat cggcctaggt gctcggggga cgattacgca aaccgatgga gtgaccaccg
     3721 tcggtcacag cctcaacgtc ggcatcaacg gcggcaccgg gacatacaac atcaaacaga
     3781 atgcgacggt cattgcgcca accctatacc tcgctagcgg cagcttcagc ttcgggatcg
     3841 tcaacatcag cgacgccgcg tcgctcaccg ttgccgacgt gtcgaaggtc ttcaacgaca
     3901 acagcaagat caacctctcg ggcggcacct tcaagaccgg ctcgctggac ctgggcggtg
     3961 tctcgtcgcg cttgaactgg acgggcggca cgctttggct gaccggcggg accattacga
     4021 gcgggaccac cccgctgagc gttggcggca cgttcaaagg caccggcacg gtgagcggag
     4081 cgacctcgat ccttgcgggc gggcgcgtgt cgcccggcga cggcgtcggg acactgcatc
     4141 tggctgatac aaccttcgcc gccgcctcaa gcctggactt ggaactgggc ggcagcggcg
     4201 cggggcagtt cgacgtgctc gacgtcgccg gctccgttgc gctggccggc cccagcctga
     4261 acgtgtcgct gatcaacggc tacacgccgc cgatcggctc gtcgttcacg gtcatcacca
     4321 acgatggcag cgacgccatc accggaacgt ttgccggcgt gaatcagggc agcgcgttgc
     4381 gtgtcgatcg caatggacat ccgaattttt tccgcgtcag ctacatcggc ggaaccggca
     4441 acgatgtgac gctcaccacc atcgaaatgc ccggagatgc caacagcgac cagaaggtgg
     4501 acgtgggcga cctgggtgtt ttggcaacca attacaacac cacggccggc gcgacctggg
     4561 cgatggggga ttttgatgga aatgggaggg tggatgtcgc ggacctgggg gcgctggcga
     4621 cgtactacgg gcaaagctcg gccgacagcc cggcgccggc gttggcgttg agcagcacta
     4681 gcgcggtccc ggagccggcg gcgacggggt tggcagcgat ggttgtggtg ggcttcggcc
     4741 ggcggatgcg ggggcgcgca ggccgtcgtt agaccgagat acgtgattgc gcccccttag
     4801 cgtacacctc tgctgcgcta cttaccgcac gatgcgatca caatctgccc tgcttggccg
     4861 cgggcgaacc gttcatttcc ccagttgaca ggcgctcggc ggcacttaac ttcggggcta
     4921 gtggaaaccg taaaaccgcg gaggaaccgg cggaaagtac gttcttatgg ac
//
LOCUS       DATIMH010001070         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4878596, whole genome shotgun sequence.
ACCESSION   DATIMH010001070 DATIMH010000000
VERSION     DATIMH010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4878596"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1370)
                     /locus_tag="VLI90_18505"
     CDS             complement(120..1370)
                     /locus_tag="VLI90_18505"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HSV16261.1"
                     /translation="MGFFEVSSILITLAAVFSFINFRYIRLPSTIGVMLIALIASLLA
                     LIVGEWWPAARHIAAEIVNGIDFNRALLHGMLAFLLFAGALHVDLGDLVEQWVPVTLL
                     ALLGTAMSTVIVGGLMRLALYWLGSDISTIDCMLFGALISPTDPVAVLSIMKSVGAPK
                     RLETQLAAESLFNDGVGVVIFMTLLSIALDHTRSPTVAGVTGLFLQQAGGGLALGMVC
                     GMITYQLLRRVDHYQLEVLLTLALAMGGYALADELHVSAPIAAVVSGLFIGNRGRAFA
                     MSDTTRVNLDLFWELIDETLNVVLFMLVGLEILSMPFIGRWHAAIAIAVIVTLAARWF
                     SVAMVVGSIRRRRPLHRGTITVLTWGGLRGGISVAMALSLPPGPHRGIIIAMTYGVVV
                     FSILVQGVTIGKVVQRVVTTEAPA"
     gene            complement(1621..2055)
                     /locus_tag="VLI90_18510"
     CDS             complement(1621..2055)
                     /locus_tag="VLI90_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16262.1"
                     /translation="MAKAKKSEQTESPAAAKDSASAAAAKKKPAKGKAPATAAPTTPL
                     IDTGLAAETAAKMIGAKVAGPAGGQPAAAGGAKETSAFKNLKANLNKPASSTMSNFLD
                     KTAGPGQKKQNLPFGGGKQVGRNQTFGADVNRAGVPRRTGGG"
     gene            2211..2879
                     /locus_tag="VLI90_18515"
     CDS             2211..2879
                     /locus_tag="VLI90_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16263.1"
                     /translation="MDKQPTEPDGGPIDFGPDEPGSGDEQHVEPEYEHVDPTDCFSPP
                     KEPCECYCLHCGRVFASDEIWFQRIIGNGSGLDGFWMCPTPNCSGAGFTFDIFPTDPA
                     HPANSGWTDDDGEPEGDEDETWDEDDDAAERFAQKEWDPAEADYATMNDDDLEGEEWK
                     LGLQPGERPPRAQWADQARKQWEEEQRRYDGPDERPRELDWSDRPDRSIPPGETDGFD
                     DIPF"
BASE COUNT          547 a          971 c          916 g          497 t
ORIGIN      
        1 cgccggtcta ccggcggtgc cgtttcggac ggatggaccg tgagtgacat cggccccgcc
       61 gcttgcggct tcgcagacca cgccgcctgg attgcgaagc cgcgagcggc ggggattcgt
      121 catgccggcg cttccgtcgt cacgacgcgt tgaaccacct tcccaatggt caccccctgc
      181 accaggatcg aaaacaccac gacgccgtac gtcatcgcga tgatgatccc gcgatgtggt
      241 ccagggggca gcgacagcgc cattgccacg gaaatcccac cgcgcaggcc gccccaggtg
      301 aggacggtga tggtcccccg gtgcagcggg cggcgccggc ggatgctgcc gaccaccatc
      361 gccacgctga accatcgcgc cgccagcgtg acgatcaccg cgatggcgat cgccgcgtgc
      421 cagcggccga tgaacggcat cgacaggatc tccagcccca ccagcatgaa cagcacgacg
      481 ttgagcgtct cgtcgatcag ctcccagaac aggtccaggt ttacgcgcgt cgtgtcgctc
      541 atcgcgaacg cgcggccgcg gttgccgatg aagaggcccg agacgacggc agcgatcggc
      601 gccgacacgt gcaactcgtc cgccagcgcg tatccaccca tcgccagcgc cagcgtcagc
      661 agcacttcaa gctggtaatg atccacccgg cggagcagtt ggtacgtgat catcccgcag
      721 accatcccca gcgccagccc gccgccggct tgttgcagga acagccccgt cacgccggcg
      781 acggtggggg atcgcgtgtg gtcgagcgcg atcgacagca gcgtcatgaa gatgacgacg
      841 ccgacgccgt cgttgaacag cgactccgcc gccaattgcg tctccagccg cttgggtgcg
      901 ccgacgcttt tcatgatgct gaggaccgcg accggatcgg tcggcgagat cagcgcgccg
      961 aacagcatgc agtcgatggt ggagatgtcg ctgccgagcc agtacagcgc cagccgcatc
     1021 aagccgccga cgatgacggt ggacatggcg gtgccgagca gcgccaaaag cgtgacgggc
     1081 acccattgtt cgaccaaatc gccaagatcg acgtgcagcg ccccggcgaa cagcaggaac
     1141 gccagcatcc cgtgcaacag cgcgcggttg aagtcgatgc cgttgacgat ctcagcggcg
     1201 atgtggcgcg cggccggcca ccactcgccg acgatcagcg ccagcagcga cgcgatcagc
     1261 gcgattaaca tcacgccgat cgtggacggc agacggatgt aacggaagtt gatgaagcta
     1321 aagacggcgg cgagcgtgat gaggatgctc gacacctcga agaagcccat gcgcgtcgcg
     1381 ctccgcggca gatgcaatct tacacgccgg gttcggcgcg tactcgcgac gaacccggat
     1441 gctgcttaaa cgcagaagga tggatgcaga atgcagaatg aaagcaggag attcggctcc
     1501 tcttcattct gcatttatca ttcatcattc tgcattgcgg cactcgatcc gggtcgtcgc
     1561 gagtacgtcc gaacccggcg tggtcgcgcg tggaatggtc tctagaaact cggcgcctga
     1621 tcagccgccg ccggtgcggc gaggcacgcc ggcgcgattc acatcggccc cgaacgtctg
     1681 attacgcccg acctgcttcc cacctccgaa cggcaggttc tgtttcttct gccccggccc
     1741 cgcggtcttg tccaggaagt tcgacatcgt cgaagacgcc ggcttgttca ggttcgcctt
     1801 gaggttcttg aacgccgaag tctctttggc gccgcccgcc gccgcgggct gaccgcccgc
     1861 cggtcctgca acttttgcac cgatcatctt cgcggcagtc tcagccgcca aaccggtgtc
     1921 gatcaacggc gtcgtgggcg ccgccgtcgc gggggctttg cccttggcgg gctttttttt
     1981 cgcggctgca gcactcgccg aatccttcgc tgccgccggg ctttccgtct gctccgactt
     2041 cttcgctttg gccatgggtt cctcttgtat gcctgtgtgc tagttgcggc cgattctagg
     2101 tatccgccgc cgcgcggcaa tgcgctgcac cgcttgcgac attcgaagct gtcagatttc
     2161 gatcgttttt tcttgcatcc gcggcgatta cccgccatcc tgaatggttg atggacaagc
     2221 agcccaccga acctgacggt ggtccgatcg atttcggccc cgacgaaccc ggcagcggcg
     2281 acgaacagca cgtcgagccg gaatatgagc acgtcgatcc caccgactgc ttctcaccgc
     2341 cgaaggagcc gtgcgagtgc tactgcctgc actgcggccg cgtcttcgcc agcgatgaaa
     2401 tctggtttca gcggatcatc ggcaacggct cgggcctcga cggtttctgg atgtgcccca
     2461 cccccaattg cagcggggcc ggcttcacgt tcgacatctt ccccaccgat cccgcgcacc
     2521 cagccaactc cggttggacg gacgacgatg gcgagcccga aggcgatgaa gacgaaacct
     2581 gggacgagga cgacgacgcc gccgagcggt tcgcccaaaa ggagtgggac cccgccgaag
     2641 ccgactacgc cactatgaac gacgacgatc tggaaggcga agagtggaag ctgggcctgc
     2701 aacccggcga acgcccgcca cgggcgcagt gggcggacca agcgcgcaag caatgggaag
     2761 aagaacaacg ccgctacgac gggcccgatg agcgcccgcg cgaactggat tggtcggatc
     2821 ggccggatcg gtcgattccg cctggcgaaa ccgatgggtt tgatgatatt ccgttttagg
     2881 gaactgtcat cctgagcgca ggcgtactcg ccggagtcga aggatcttgc c
//
LOCUS       DATIMH010001071         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_544801, whole genome shotgun sequence.
ACCESSION   DATIMH010001071 DATIMH010000000
VERSION     DATIMH010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544801"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VLI90_18520"
     CDS             <1..963
                     /locus_tag="VLI90_18520"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HSV16264.1"
                     /translation="FWALNIAVIWRGIETIKFLEGIGAPFMLGIGLLLLWWITSKAGG
                     FGPVLSAPSKFHTPAEFFRFFIPSLTGMVGFWATVALNIPDFTRYAKSQKAQAMGQAI
                     GLPTAMTLYSFIGVAVTSASVVLFGEAIWDPVTLLGKFNQPVVAFIALIALLIATLNT
                     NVAANVVSPSNDFSNLNPRLISFRTGGLITGVIGVLMMPWKLLTDFSSYIFGWLVGYS
                     GLLGPIAGVMIVDYFVVRRSRLRVEDLYRRDGIYAYENGVNLRAIAALAIGIAVALLG
                     LFVPALRLLYDYAWFVGFGVSGAVYRLLMQGATVPAIPDWEGEA"
     gene            960..>2124
                     /gene="mmsA"
                     /locus_tag="VLI90_18525"
     CDS             960..>2124
                     /gene="mmsA"
                     /locus_tag="VLI90_18525"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSV16265.1"
                     /translation="MIATGNAVGTVSFFVNGVWEKLDGRTTGAVTNPATGEKIAEVPY
                     ANEADIDRAVRAAHAAFLKWRDVPVVDRVQVLYRYKTLLDQHADEIAAILTRENGKTA
                     DDAKMEVRRMIQMVEVACGMPTLMMGDSLNDVAQGIDCKTIRQPIGVCAGITPFNFPA
                     MVPTWMYPFAIACGNTFVLKPSEKVPLTPTRAIELLHDAGLPPGVLNLVHGGKEAVDA
                     LLHHPLVKAVSFVGSTPIAKYIYATAAAEGKRVQALGGAKNHLVVMPDADMPKTVEAI
                     IGSAFGAAGERCLAGSVLVTVGEAAAPLLELLVKRTKGLAVGDGAKSGVEMGPLVTGE
                     HCAKVAGYVDKGVAEGATPLVDGRGGKTDNGGFFLGPTIFDNVTAEMTIAKEEI"
BASE COUNT          366 a          639 c          702 g          417 t
ORIGIN      
        1 ttttgggcgc tcaacatcgc ggtgatctgg cgcggcatcg agaccatcaa gttcctcgaa
       61 ggcatcggcg cgccattcat gctgggcatc ggactgctgc tgctctggtg gatcaccagt
      121 aaggccggcg gcttcggtcc ggtgctgagc gcgcccagca aattccatac gcccgccgag
      181 ttcttccgat tctttattcc gtcgctgacc ggtatggtgg ggttctgggc gacggtcgcg
      241 ttgaacatcc ccgacttcac ccgttacgcc aaatcgcaga aggcgcaagc gatgggacag
      301 gccatcgggc tgccgaccgc gatgacgctg tactcgttca tcggcgtggc cgtgacgtcg
      361 gcgtcggtgg tgctgttcgg cgaggcgatc tgggatccgg tgacgctgct gggaaagttc
      421 aaccaaccgg tggtggcctt catcgcgctg atcgcgctgc tcatcgcgac gctgaatacg
      481 aacgtcgcgg cgaacgtcgt gtcgccttcg aacgatttct cgaatctgaa tccgcggttg
      541 atttcgttcc gcaccggcgg cctgatcacc ggcgtgatcg gcgtgctcat gatgccgtgg
      601 aaactgctga ccgatttcag ctcgtacatt ttcggctggc tggtggggta ctcgggactg
      661 ctgggtccca tcgccggcgt gatgattgtg gactatttcg tggtgcggcg ctcgcggtta
      721 cgcgtcgagg acctctaccg gcgcgacggc atctacgcct atgaaaacgg cgtcaacttg
      781 cgggccatcg ccgcgctggc gatcggaatt gcggtcgcgc tgctggggct cttcgttccc
      841 gccctgcggc tgctgtacga ctatgcctgg tttgtcggct tcggtgtttc cggggccgtg
      901 tatcgtttgc tgatgcaggg cgcgactgtc cctgcaattc cggattggga aggtgaagca
      961 tgattgccac aggaaatgca gttggaaccg tttcgttttt cgtcaacggc gtttgggaga
     1021 aactcgacgg gcgcaccacg ggtgccgtga ccaacccggc caccggcgaa aaaatcgctg
     1081 aggtgccgta cgctaatgag gccgatatcg atcgggcggt ccgcgccgcc cacgcggcgt
     1141 tcctcaagtg gcgcgatgtg cccgtggtcg atcgcgtaca ggtgctgtat cgctataaga
     1201 cgctgctgga tcagcatgcc gacgagatcg ccgccatcct cacgcgcgag aacggcaaga
     1261 cggccgacga cgccaaaatg gaagtccgcc gcatgatcca gatggtcgaa gtggcctgcg
     1321 gcatgccgac cctcatgatg ggcgattcgc tcaacgacgt ggcgcaaggt atcgactgca
     1381 agaccattcg ccagccgatc ggcgtctgcg cgggcattac gccgttcaat ttccccgcca
     1441 tggtgcccac gtggatgtat cccttcgcca tcgcctgcgg aaatacgttc gtgctgaagc
     1501 cgtcggaaaa ggttcccctg acaccgactc gcgccatcga actgttgcac gatgccgggc
     1561 tgcctccggg ggtcctcaac ctggtacacg gcgggaagga agcggtggac gcgctgctgc
     1621 atcatcccct cgtaaaggcc gtgtcgttcg tcggctcgac gccgatcgcg aagtacattt
     1681 acgccaccgc cgcggcggag ggcaagcgcg tgcaggcact gggcggcgca aagaatcacc
     1741 tggtcgtcat gcccgacgcc gacatgccga agaccgtcga ggcgatcatc gggtcggcat
     1801 tcggcgccgc gggagagcgc tgcctggccg gcagtgtgct cgtcacggtg ggcgaggccg
     1861 ccgcgccgct tctcgaattg ctggttaagc ggacaaaggg gctcgcggtc ggcgatggcg
     1921 caaagagcgg cgtcgagatg ggcccgctgg tgaccggcga gcactgcgcc aaagtcgcgg
     1981 gatacgtcga caagggcgtc gcggagggcg ccacgcctct ggtggacggc cgcggcggta
     2041 aaacggacaa cggcggattc tttctgggtc ccacgatctt cgacaacgtg accgccgaga
     2101 tgactatcgc caaggaagag atct
//
LOCUS       DATIMH010001072         3419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1993382, whole genome shotgun sequence.
ACCESSION   DATIMH010001072 DATIMH010000000
VERSION     DATIMH010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993382"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VLI90_18530"
     CDS             complement(<1..616)
                     /locus_tag="VLI90_18530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV16266.1"
                     /translation="MPRRHTLLVVDDEPDVVKSVQDLLRLDYRVLTTTRAADGIKIME
                     REEVHVVMTDQRMPGMTGVEFLKNIRGDHPEAIRLLFTGYADIKAVIDAINAGNVYRY
                     LTKPWDPDELQAVVREACERYDLIAERKQLIEDLKINNEKLEKANKALSEASALKSAF
                     IQVASHELRTPLTILLGLSDLAERAPGLQDPLRGWLGRIKAASNR"
     gene            774..2042
                     /gene="purD"
                     /locus_tag="VLI90_18535"
     CDS             774..2042
                     /gene="purD"
                     /locus_tag="VLI90_18535"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HSV16267.1"
                     /translation="MKVLLIGGGGREHAIAWKLADSPRVTELYALPGNPGIAQVADLV
                     PGSVTDFSAIEAFIKKAAIEFVVIGPEDPLAAGLADRISAMGVKVFGPNKAAAQLEAD
                     KWFAKEIMRHQAIPTAEARSFTDASAAEEYVKVRNEACVVKAAGLAKGKGVTVCYRVS
                     DALETIDAIMRKKTFGGAGNRIVIEELLQGPECSILAFVDRRNIYAMEPSQDHKPVDD
                     GDTGPMTGGMGAYSPTPVVTDAMLGQIERDVLVPIVDGLLREGIDYKGVLYAGLMLTT
                     NGPKVLEFNCRFGDPETQPLMMRLKSDLLEVMLAVADGKLDQVELKWDERPAVCVVAT
                     SKGYPGSYPTGVPITGIEKADALPDVKVFHSGTRAEGDTIVTDGGRVLGVTALGRTTL
                     EAQRRAYQAMDLIHFDGMHFRRDIAHQAVR"
     gene            complement(2067..>3419)
                     /locus_tag="VLI90_18540"
     CDS             complement(2067..>3419)
                     /locus_tag="VLI90_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16268.1"
                     /translation="SIHRLIVITNFALALFGAAGAAALGIYYGGIGAGILVGGLFAVV
                     PVFLQLSEMSRPYSMAWSFGMLSLCCAAICASRWRVWLAGAFLGLAIASRIEMLCLLP
                     LVWWIVCERRENSRRIVALVNVMLVSLAVAILVAPWLLTHLIGNLRTIATVRLLGPPG
                     GKAPWASALWEIVFRQGMGIAMVLALAGLVLQPAGARRRAWLLTIYAALLLGSLLVGD
                     SFGVHQHGEVFIALFLLISVTFGAAGTKWNRASPWIVAVALMLPFAQAVRLIRSVRAA
                     TVEHEDVPRWIEQHVPAGTTVYLMPGGMRSLLPTTQASDALWKEVTDEQAWRAKMASG
                     LSRFHLSAGDLPRALADENLVQERGNRRQWFILGSRTDLPDPRYDIRVVRGSPVFGIR
                     DPAVAMKQSGGVLVFRDGNDLPRPEEFGPPLVSWLNRDGGGVLIYCTPDLREKIKSDP
                     "
BASE COUNT          686 a         1053 c         1114 g          566 t
ORIGIN      
        1 ggcgattgct cgccgccttg atgcggccga gccagccgcg cagcggatcc tgcaggcccg
       61 gcgcgcgctc ggcgagatcg ctcaagccca acaggatcgt aaggggcgta cgcagttcgt
      121 ggctggcgac ctggatgaat gcagatttca gtgcgctcgc ttcgctcaaa gccttattcg
      181 ccttctccag cttttcgttg ttgatcttca gatcctcgat cagctgtttc cgctcggcga
      241 tcagatcgta ccgctcgcac gcctcgcgga cgaccgcctg caattcgtcg ggatcccacg
      301 gcttggtcag gtagcgatag acgttcccgg cgttgatggc gtcgatgacc gctttgatat
      361 cggcgtagcc agtgaacagc aggcggatgg cttcgggatg atcgccgcgg atgttcttga
      421 ggaattcgac gccggtcatg ccgggcatgc gctggtcggt catgacgacg tgaacttctt
      481 cgcgctccat gatcttgatg ccgtcggcgg cgcgcgtggt ggtgagcacg cgatagtcca
      541 ggcggagcag atcctgaacg ctcttgacga cgtccggttc gtcatcgacg acaagcagcg
      601 tgtggcgacg tggcatgcgc gagatattgt aacgcgaatg aaccgcagcc gcttgcggct
      661 tagcatctgt ttcttccaat cgaaccgacg ctaagccgca accggcggca ttgcgacggc
      721 ggcgcggatc gataagcttc ggcgtttgac ccgtttctca ggaagcattc gcgatgaaag
      781 tactattgat cggcggcggt ggcagggagc acgcgatcgc gtggaagttg gcggattcgc
      841 cgcgcgtgac ggagctgtac gcgctgcccg gcaaccccgg catcgcgcag gtggcggacc
      901 tggttcccgg gagcgtcacc gactttagcg cgatcgaagc gttcatcaaa aaggcggcga
      961 tcgaattcgt cgtgatcggt cccgaagacc cgcttgccgc ggggttggcg gatcgaatca
     1021 gcgcgatggg cgtgaaggtg ttcggcccca acaaagcagc cgcgcagctg gaagcggata
     1081 agtggttcgc gaaggaaatc atgcgccacc aggcgatccc caccgccgag gcgcgcagct
     1141 tcaccgatgc gtcggcggcg gaggaatacg tgaaggtgcg caacgaggcg tgcgtagtga
     1201 aagccgcggg gttggccaag ggaaaaggcg tgaccgtctg ctatcgcgtc agcgatgcgc
     1261 tggagacgat cgacgcgatc atgcgcaaga agacgttcgg cggcgccggc aatcgcatcg
     1321 tgatcgaaga gctgctgcag gggccggagt gttcgatcct agccttcgtc gatcggcgaa
     1381 acatttacgc gatggaaccg agccaggacc acaagccggt ggacgacggc gacaccggcc
     1441 cgatgaccgg cgggatgggc gcgtattcac cgacgccggt agtgaccgac gcgatgctcg
     1501 gacagatcga gcgcgacgtg ctggtaccca ttgtcgatgg actgctgcgc gaaggcatcg
     1561 actacaaagg cgtgctgtac gcgggcctga tgctgacgac gaacgggccg aaggtgctgg
     1621 agttcaattg ccgattcggc gatcccgaaa cgcagccgct gatgatgcgg ttgaagagcg
     1681 atctgctgga ggtgatgctc gcggttgccg atgggaagct cgatcaggtc gagctgaagt
     1741 gggacgaacg tcccgccgtc tgcgtcgtcg cgacgagcaa gggttatcca ggttcatatc
     1801 ccaccggtgt tccgatcact ggcatcgaga aagcggatgc cttgccggac gtgaaggtgt
     1861 ttcacagtgg aacgcgcgcc gaaggcgaca cgattgtaac ggacggcggg cgcgtgcttg
     1921 gcgtcacggc gctgggacgt acgacgcttg aggcgcaacg acgggcatat caggcgatgg
     1981 acttgattca cttcgacggc atgcacttcc ggcgagacat cgcgcatcag gccgtccgat
     2041 agcgcgcccg atgctgcggc gagcgattac gggtccgatt tgatcttctc gcgcagatcc
     2101 ggcgtgcaat aaatcaatac gcccccaccg tcgcgattga gccacgaaac cagcggcgga
     2161 ccaaattcct cgggacgcgg caagtcattg ccgtcgcgga acacgagcac gcccccgctt
     2221 tgcttcatcg ccaccgccgg gtcgcgaatg ccgaagacgg gcgagccgcg cacgacgcgg
     2281 atgtcgtagc gtgggtcggg caggtcggtt cggctgccga ggataaacca ttgccgccga
     2341 ttgccgcgct cctgcacgag gttttcgtca gccaacgcgc gcggcagatc gccggcagac
     2401 aaatgaaacc ggctgagccc cgatgccatc ttcgcccgcc aggcctgctc gtccgtcact
     2461 tccttccaca aggcgtccga agcctgcgtc gtcggcaaca acgatcgcat cccgccgggc
     2521 atcaggtaaa ccgtcgtgcc ggcgggaacg tgctgctcga tccagcgggg aacatcttcg
     2581 tgctcgacgg tggcggcgcg cacggatcga atcagtcgca cggcctgcgc gaatggaagc
     2641 atcagcgcca cggcgacgat ccacgggctg gcgcgattcc atttcgtgcc cgccgcgcca
     2701 aacgtcacag atatcagaag gaaaagcgcg atgaacactt ctccatgctg gtgaacgccg
     2761 aagctatcgc cgaccaacag gctgccgagc agcagcgctg cgtaaatcgt cagcaaccac
     2821 gctcgccggc gcgcgccggc cggttgcagc accaggcccg ccagcgccag caccatcgcg
     2881 atgcccatac cctggcgaaa gacgatctcc cacagcgctg aagcccaagg cgctttccca
     2941 cccgggggac cgagcaaccg cacggtggcg atcgtccgca ggttgccgat cagatgggtg
     3001 agcagccagg gcgccaccaa gatcgcaacc gccagagaaa caagcatgac gtttacgagc
     3061 gccacgattc gccggctgtt ctcgcggcgc tcgcacacga tccaccagac cagcggcagc
     3121 aggcagagca tctcgatgcg tgatgcgatc gccaagccca gaaatgcgcc ggcgagccac
     3181 acgcgccagc gcgacgcgca gatcgccgcg caacacagcg agagcatgcc gaacgaccag
     3241 gccatcgaat acggtcgcga catttcgctg agctgaagaa agaccggcac gaccgcgaag
     3301 aggcctccga ccagaatccc ggccccgatg ccaccgtagt aaatgcccag agccgccgcg
     3361 ccggcggctc caaagagcgc cagcgcgaag ttggtgatga cgattaacct gtggatcga
//
LOCUS       DATIMH010001073         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4586087, whole genome shotgun sequence.
ACCESSION   DATIMH010001073 DATIMH010000000
VERSION     DATIMH010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4586087"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3735
                     /gene="gltB"
                     /locus_tag="VLI90_18545"
     CDS             <1..3735
                     /gene="gltB"
                     /locus_tag="VLI90_18545"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HSV16269.1"
                     /translation="GSDSATLDNMIQFLAVNGRSLPHAVLMAIPEAWQHNHLMDPDLK
                     AFYEYHACLMEPWDGPASISFTNGSVIGAVLDRNGLRPSRYYVTKDDLVIMASEVGVL
                     PIDPSRVLRKWRLQPGKIFLIDMKQGRIIDDNEIKRELVDKRPWRKWLDENLIPLDDL
                     PAPSNVPTPDHDTLLVRQHAFGYTNEDVKYILGPMCRDGQEGLGSMGTDTPLACLSER
                     PQLLYNYFKQLFAQVTNPPLDALREELVTSLYTYLGREGNLLDETPKHCHLIKLQQPI
                     LSNADLAKLREVSKGELRSVTIPMLFNVADGPAGLGKAMDEMCAAVSAAVKEGASIAI
                     LSDRGVDRDKAPIPSLLATAAVHHHLIREGTRTQCGLVLETGEAREAHHFCLLIGYGA
                     GAINPYVAFESIADMHREKMFPPELSFDQAKKNYIKAANKSIIKVASKMGISTVQSYR
                     GAQIFEAVGIARDVIDRYFTGTASRIQGVTLETIAQESLARHHRGYPPIEVDGDTLDN
                     GGQYQWRKFGEYHMWNPDTVAKLQQAVRITGYPTFKEYSKLIDDESKHRCTIRGLLNF
                     KKSGTPIPLEEVEPAKEIVKRFVTGAMSFGSISKEAHENLAIAMNAIGGKSNTGEGGE
                     DPDRFQPDKGATLSHITVKGTKTELPDQPVWRRSAIKQVASGRFGVTIEYLVNCQELQ
                     IKMAQGAKPGEGGQLPGHKVDEYVGKIRHSTPGVGLISPPPHHDIYSIEDLAQLIHDL
                     KSSNPAARVSVKLVSEVGVGTIAAGVAKAKADHILISGDSGGTGASPLTSIKYAGLPW
                     ELGLAETQQTLVLNGLRGRVIVQTDGQLKTGRDVAIAALLGAEEFGFSTGPLIASGCI
                     MMRVCHLNTCPVGIATQDPELRKKFMGKPEHVINFMFFVAEEVREIMAELGIRKMEDM
                     VGRSELLEFGDLSDHWKAKNVDLSPILYRPEPKIGDGALHNIGKQDHGLENSLDYKVL
                     IDAARPAIERGEKVIKEFAIKNTNRTVGTTLSGEIAKRYGQVGLPDDTITFKFTGHAG
                     QSFGCFLAHGVTLLLEGDANDYVGKGLSGGRIAVFPPKEATFKPGENIIAGNTILYGA
                     IDGEMYLRGVVGERFCVRNSGASAVVEGVGDHGCEYMTGGRVVVIGPTGRNFAAGMSG
                     GIAYVYDEGAQFDKLCNQDMVSLEKPDKPEDRQTIKQLLENHHKYTGSPRAKEILDDF
                     EKEIRWFVKVMPNEYRNVLEHMAEIEERAKKLGQRQTATV"
     gene            3749..>4229
                     /locus_tag="VLI90_18550"
     CDS             3749..>4229
                     /locus_tag="VLI90_18550"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV16270.1"
                     /translation="MINAMSIIAPISPGSGAAFRFGMADEWHHALGDVPLKRVVFDPL
                     PGTATEYDQIFRVERQHRFAELIDGTLVEKPMGSFESMIAAIIAKAILVFLEKHDLGV
                     VTGESAMMRMANGRVRMPDAAFTSYDRLPERRPPRDPIWPLSPDLAVEVLSQSNTKAE
                     "
BASE COUNT          895 a         1200 c         1437 g          697 t
ORIGIN      
        1 ggttcggact cggccacgct ggacaacatg atccagttcc tggcggtgaa tggccgcagc
       61 ctgccgcacg cggtgctgat ggccatcccc gaagcctggc agcacaacca cctgatggac
      121 ccggacctga aggcgtttta cgaatatcac gcctgcctga tggaaccgtg ggacggcccg
      181 gcgagcattt cgttcaccaa cggcagcgtg atcggcgcgg tgctggaccg aaacgggctt
      241 cgtcccagtc gctattacgt caccaaggac gacctggtga tcatggccag cgaagtcggc
      301 gtgctgccga tcgacccgtc gcgtgtattg cgaaagtgga gattgcaacc aggcaagatc
      361 ttcctgatcg acatgaagca gggtcggatc atcgacgaca acgagatcaa gcgcgagctg
      421 gtcgacaagc gtccctggcg gaagtggctg gacgagaacc tgattccgct ggacgacctg
      481 ccggcgccga gcaacgtgcc gacgccggat catgacacgc tgctggttcg ccagcacgcg
      541 tttgggtaca cgaacgaaga cgtcaaatac atcctgggcc cgatgtgtcg cgatgggcag
      601 gagggcttgg gttcgatggg caccgacacg ccgctggcgt gcctgagcga gcgcccgcag
      661 ctgctgtaca actatttcaa gcagctgttc gcgcaggtga ccaacccgcc gctggacgcg
      721 ctgcgggagg agttggtcac gtcgctgtac acgtatctgg ggcgggaagg caacctcctc
      781 gacgagacgc cgaagcattg ccacctgatc aagctgcagc agccgatcct gagcaacgcg
      841 gacctggcga agctgcgcga ggtgagcaag ggcgagctgc gatcagtgac gatcccgatg
      901 ctgttcaacg tcgccgatgg cccggccggg ctgggcaagg cgatggacga gatgtgcgcc
      961 gcggtgagcg cggcggtgaa ggaaggcgcg tcgatcgcca tcctgtccga tcgcggggtc
     1021 gatcgcgaca aggcgccgat ccccagcctg ctggcgacgg cggcggtgca tcatcacctg
     1081 atccgcgaag gcacgcgcac gcagtgcggg ctggtgctgg agaccggcga agcgcgcgag
     1141 gcgcatcatt tctgcctgct gatcggttac ggggcgggcg cgatcaatcc gtatgtcgcg
     1201 ttcgaatcga tcgccgacat gcaccgcgaa aagatgttcc cgccggagtt gagcttcgat
     1261 caggcgaaga agaattacat caaggcggcc aacaagagca tcatcaaggt cgcgtcgaag
     1321 atgggcatct cgacggtgca aagctaccgc ggggcgcaga ttttcgaagc ggtcggcatc
     1381 gcgcgcgatg tgatcgaccg ctacttcacc ggcacggcca gccggatcca gggcgtgacg
     1441 ctggagacga tcgcgcagga gtcgctggcg cggcatcatc gcgggtatcc gccgatcgaa
     1501 gtggacggtg acacgctgga caacggcggg cagtatcagt ggaggaagtt cggcgagtac
     1561 cacatgtgga accccgacac ggtcgccaag ctgcagcagg cggtccgcat caccgggtat
     1621 ccgactttca aggaatactc gaagctcatc gacgacgaga gcaagcaccg ctgcacgatt
     1681 cgcgggctgc tgaacttcaa gaagtcgggc acgccgattc cgctggaaga agtcgagccg
     1741 gcgaaggaga tcgtcaagcg cttcgtgacc ggggcgatga gcttcgggtc gatcagcaag
     1801 gaagcgcacg agaacctggc gatcgcgatg aacgcgatcg gcgggaagag caacacgggt
     1861 gaaggcggtg aagacccaga ccggttccaa ccggacaagg gcgcgacgct ttcccatatc
     1921 accgtgaagg gaacgaagac ggaattgccg gaccagccgg tctggcggcg cagcgcgatc
     1981 aagcaggtgg cgtcgggccg attcggtgtg acgatcgagt atctggtcaa ctgccaagag
     2041 ttgcagatca agatggccca gggcgccaag cccggtgaag gcgggcagct gccggggcac
     2101 aaggtggatg aatatgtcgg caagatccgc cacagcacgc cgggcgtcgg gctgatctca
     2161 ccgccgccgc atcacgatat ttattcgatt gaagatctgg cgcagctgat tcacgacttg
     2221 aagagctcga accccgcggc gcgcgtgagc gtgaagctgg tgagcgaagt cggcgtcggc
     2281 acgattgccg cgggcgtggc caaggcgaag gcggatcaca ttttgatcag cggtgacagc
     2341 ggtgggaccg gcgcgtcgcc gctgacgtcg atcaagtacg cgggattgcc gtgggaactg
     2401 ggtctggcgg aaacgcagca gacgctggtt ctgaacggac tgcgtggtcg tgtgatcgtg
     2461 cagacggatg gacaattgaa gactggtcgc gacgtggcga tcgccgcgct gctaggggcc
     2521 gaagaattcg gcttcagcac cgggccgctg attgcatccg gctgcatcat gatgcgcgtg
     2581 tgtcatctca acacctgccc ggtcggcatc gccacgcagg acccggagct tcgcaagaag
     2641 ttcatgggca agcccgagca tgtgatcaac ttcatgttct tcgtggccga agaggtgcgc
     2701 gagatcatgg cggagttggg catccgcaag atggaagaca tggtcggccg cagcgaattg
     2761 ctggagttcg gcgacctgtc cgatcactgg aaggccaaga acgtggacct gtcgccgatt
     2821 ctgtatcgac cggagccgaa gatcggcgac ggcgcgctgc acaacatcgg caagcaggat
     2881 catgggttag aaaactcgct cgattacaag gtgctgatcg acgccgctcg tcccgcgatc
     2941 gagcgcggcg agaaggtgat caaagagttc gcgatcaaga atacgaatcg caccgttggc
     3001 acgacgctga gcggtgagat cgccaagcgg tacggccagg tcggcctgcc ggacgacacg
     3061 atcaccttca agttcaccgg ccacgcgggc cagagcttcg ggtgcttcct ggcgcacggc
     3121 gtgacgctgc tgctggaagg cgacgccaat gattacgtcg gcaagggcct gagcggcggg
     3181 cgaatcgcgg tcttcccgcc gaaggaagcg acgttcaaac cgggcgagaa catcatcgcc
     3241 ggcaacacga ttttgtacgg cgcgatcgac ggcgagatgt atttgcgcgg cgtcgtcggc
     3301 gagcgcttct gcgtgcgcaa cagcggcgcc agcgcggtcg tcgaaggcgt gggcgatcac
     3361 ggttgcgaat acatgaccgg cgggcgcgtc gtcgtcatcg gaccgacggg aagaaacttc
     3421 gccgccggca tgagcggcgg catcgcgtac gtctacgacg aaggcgcgca gtttgacaag
     3481 ctgtgcaacc aggacatggt cagcctggaa aagccggaca agccggaaga tcgccagacg
     3541 atcaagcagc tgctggagaa ccatcacaag tacaccggct cgccgcgggc gaaggagatt
     3601 ttggacgatt tcgagaagga gatccgctgg ttcgtgaagg tgatgccgaa cgaataccgc
     3661 aacgtgctgg agcacatggc ggagatcgaa gagcgggcga agaagctcgg ccagcggcag
     3721 acggcgacgg tataatccag agctaagttt gatcaacgcg atgtccataa ttgcacctat
     3781 ttcgccgggc tccggggccg ccttcagatt cggaatggcc gacgagtggc accatgcgct
     3841 gggcgacgtc ccgctgaagc gggttgtgtt cgatccgctg ccgggaacag cgacggagta
     3901 cgaccagatt ttccgggtcg aacgtcagca ccgcttcgct gagctgatcg acgggacgct
     3961 ggtggagaaa ccgatgggat cctttgaatc gatgattgcg gcgatcatcg ccaaggcgat
     4021 cctcgttttt ctggagaagc acgacctggg tgtggtaacc ggcgaatccg ccatgatgcg
     4081 aatggcgaac ggccgtgttc gcatgccgga tgcggcgttc accagttatg atcggctgcc
     4141 cgaacgccgt ccaccgcgcg acccaatctg gccgctatcg cctgatctgg ccgttgaagt
     4201 gctaagccag agcaatacta aggcggaga
//
LOCUS       DATIMH010001074         4628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1993718, whole genome shotgun sequence.
ACCESSION   DATIMH010001074 DATIMH010000000
VERSION     DATIMH010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993718"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VLI90_18555"
     CDS             complement(<1..447)
                     /locus_tag="VLI90_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16271.1"
                     /translation="MSYPISADAAIAPPPAPAVTVAPRIEIPHITAVESPAGKLPRLV
                     EEPAADRWIYGVLALALVVGYYIALHTYWVPAHPGVDQNGYLVGGKFFASTWSTGFKP
                     ADPFSFVGRMWVEAQNGRFYPKYPLGLSVIYAAAMKIGGARYGLSLA"
     gene            complement(703..1641)
                     /gene="murQ"
                     /locus_tag="VLI90_18560"
     CDS             complement(703..1641)
                     /gene="murQ"
                     /locus_tag="VLI90_18560"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HSV16272.1"
                     /translation="MLGMGETPMLHMNDRSHLLTELRLRESMSLDAMSVDDAVALMNA
                     QDVVAVAAVAAVQAETVKAIDLVVAAFRVGGRLIYFGAGTSGRLGVLDASECPPTFRT
                     DPQMVQGVIAGGEAAMFRAQEGAEDRADDGAAAVDAKDVGAADVVFGIAAGGTTPFVH
                     GALRRAKERGAKTIFLSCVQPVPGEPTIDVVIRPLTGPEVVTGSTRLKAGTATKLVLN
                     TITTIAMVQLGKVYENLMVDLRATNAKLRDRATRIVATLTKLPREAAAELLQRADGHV
                     KSAVVMHHRAVDLAQATKILAACEGRLREAMVRGIE"
     gene            complement(1666..2802)
                     /locus_tag="VLI90_18565"
     CDS             complement(1666..2802)
                     /locus_tag="VLI90_18565"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HSV16273.1"
                     /translation="MAERPSRIIAGAMSGTSADGVDVALVRVDGAGLDMSARLIHHHH
                     VAYDAALRNAIFGCRNAGEIQLADLAALARQISLAYARAVNETLVAAGISAGEVAAVA
                     AHGQTLYHDPPTTIQWFDPALVAAEVGCAVVSDFRRADCAAGGQGAPLVPFADYILFC
                     DPVKHRVLLNIGGIANVTYIPAGASIDSIVAFDTGPGNCVSDFLMRQMHPEGPGVDTD
                     GVLASHGQPDEAIVLQVLSAPFFKQPPPRSTDGPAMVDLFFAVVSDIGPRGKLPNHLA
                     TASLISITAIVRAMREFLPHFPDEVIASGGGVKNHAMMNYLKAQLAHVPLRMSGEVGV
                     PGDAKEAIAFALLAAATLDGVPANVPAATGAKRRVILGSVTPKP"
     gene            3182..3562
                     /locus_tag="VLI90_18570"
     CDS             3182..3562
                     /locus_tag="VLI90_18570"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HSV16274.1"
                     /translation="MAEKSKKKTEDDAGASSQGNTGGGGATATAKPKKAMKRAPKKRP
                     PGFLPPWKVLLHNDDKNSVDFVIVTIVELTPLDKQQAELRTKEADKTGVALLLTTHKE
                     RAELYQEQFQSKGLTVTIEPAEEG"
     gene            complement(3602..3871)
                     /locus_tag="VLI90_18575"
     CDS             complement(3602..3871)
                     /locus_tag="VLI90_18575"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16275.1"
                     /translation="MGRRVRWAGSDVQNGDRVHYVTIVFRCRPVGGEPCVNDDETTDV
                     RYFKLDELPEMRPDHRRNIEQALRDDPAAVFVRRDPSSPKQKGSD"
     gene            complement(3763..4107)
                     /locus_tag="VLI90_18580"
     CDS             complement(3763..4107)
                     /locus_tag="VLI90_18580"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSV16276.1"
                     /translation="MPASEFIRNIRQKIGHDLLHLVGVSGVVITDDRRVLLVKSKEVL
                     GWMPIGGMIEPGEEPADAVVREIHEETSVEVVPERLVGVFDGPEVTYKMATASTTSRS
                     SSAAGRSVGSRA"
     gene            complement(4298..>4628)
                     /locus_tag="VLI90_18585"
     CDS             complement(4298..>4628)
                     /locus_tag="VLI90_18585"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HSV16277.1"
                     /translation="DLGAAIQGGMGMAASGNLNPTRQTASMFEPVHGSAPDIAGKNFA
                     NPIATFLSVAMMLDFLNQPKAAAAINQACKGVVANPKNHTRDLGGTATTTQVGDAVCS
                     ALDAALS"
BASE COUNT          868 a         1506 c         1429 g          825 t
ORIGIN      
        1 cgccagcgat aatccatatc gcgccccgcc gatcttcatc gcggcggcgt agatgacgga
       61 caatcccagc ggatatttcg ggtagaaccg cccgttctgt gcttccaccc acattcgccc
      121 gacaaacgaa aacggatcgg cgggcttgaa tccggtggac cacgtcgagg cgaaaaattt
      181 tccgccgacg agatagccgt tctggtcaac acccggatga gccggcaccc agtacgtgtg
      241 cagcgcgatg taatagccga cgacaagcgc gagcgccagc acgccgtaaa tccagcgatc
      301 ggcggccggc tcctccacca gacgcggcag cttccccgcc ggcgattcaa ccgcggtgat
      361 gtggggtatt tcaatgcgcg gcgcgacggt gacagcgggc gcgggcggcg gcgcgatcgc
      421 cgcgtcggcg gatattgggt aactcaaaga cgaaatggcg agctctcctg ttcgaacacc
      481 aatacgcgat gaagaacgcg gctgatctgt ctttattacc cgcgccggcc gcgcttgtgg
      541 tgttgttgga cgggcatgaa tgcgaattgt tgcaccggca gcggcagatc aacgcgttcg
      601 cgcaacggcg tagcagtcgc ttgcggtttc gcagaaggag gaagccgcag tgcttgatct
      661 gcgaaaccgc aagcgactgc cgcgcgatcc gcgcaaataa cactattcga ttccccgcac
      721 catcgcctct cgcaatcgcc cctcgcacgc cgccaggatt tttgtcgcct gcgccaaatc
      781 cacagcgcga tgatgcatca ccaccgcgct cttcacgtgc ccgtcggccc gttgcagcaa
      841 ttccgccgcc gcctcgcgcg gcagcttggt caacgtcgca acgatccgcg tcgcgcgatc
      901 gcgcagcttc gcatttgtcg cgcgcagatc gaccatgagg ttttcgtaca ccttgcccag
      961 ctgcaccatc gcgatggtcg tgatcgtgtt cagcaccagc ttcgtcgccg tgcctgcctt
     1021 gaggcgggtc gaaccggtaa ccacctccgg ccccgtgagc gggcgaatca ccacgtcgat
     1081 cgtcggttcg cccggcaccg gttgaacgca ggagagaaag atcgtcttag ccccccgctc
     1141 cttcgcgcgg cgcagcgccc catgcacgaa cggcgtcgtc cccccggcag cgatgccgaa
     1201 gacgacgtcg gccgcgccaa catctttcgc atccaccgcc gccgcgccgt cgtcggctct
     1261 gtcttccgcc ccctcctgcg cgcgaaacat cgccgcctca ccgccggcga tcacaccttg
     1321 caccatctgc ggatccgtac gaaacgtcgg cggacattcg ctggcgtcga gcaccccaag
     1381 ccggccgctg gtgccggcgc cgaagtaaat gaggcgacca ccaacccgaa acgccgcgac
     1441 caccaggtcg atcgccttga ccgtctccgc ctgaaccgcc gccaccgccg ccacggcgac
     1501 aacgtcctgc gcgttcatca acgccaccgc atcgtcaacg ctcatagcgt cgagcgacat
     1561 cgattcgcga aggcggagtt cagtcaaaag gtgtgaacgg tcgttcatat gtagcatggg
     1621 cgtctcgccc atgccgagca tcttggcatg agcgaggcgc tcatgctacg gtttgggcgt
     1681 caccgatccg agaatcactc gccgcttggc gcctgttgct gccgggacgt tggccggaac
     1741 gccatccagc gtcgccgcgg ccagcagcgc gaacgcgatc gcttctttcg cgtcgccggg
     1801 cacgcccacc tcgccactca tccgcagcgg cacgtgggcc agctgggctt tgaggtaatt
     1861 catcatcgcg tggttcttca ccccgccgcc gctggcgatg acttcgtcgg ggaaatgcgg
     1921 aaggaattcg cgcatcgcgc ggacgatcgc ggtgatgctg atcaggctcg cggtcgccag
     1981 atggttcggc aatttgccgc gcggaccgat gtcggagacg acggcgaaga agaggtcgac
     2041 catcgccggg ccatccgtgc tgcgcggcgg cggttgcttg aagaacgggg ccgacagcac
     2101 ctgcagcacg atggcctcgt cgggctggcc gtgcgacgcg agcacgccat cggtgtccac
     2161 gccggggcct tcggggtgca tctgccgcat gaggaaatcg ctgacgcaat ttcccgggcc
     2221 ggtgtcgaac gcgacgatcg aatcgatcga cgccccggcg ggaatgtacg tgacgttggc
     2281 gatgccgccg atgttcagca gcacgcggtg cttcaccgga tcgcaaaaca gaatgtaatc
     2341 cgcgaacggc accagcggcg cgccctgccc accggcggcg cagtcggcgc ggcgaaaatc
     2401 gctgacgacg gcgcatccca cttccgccgc caccagcgct gggtcgaacc attgaatggt
     2461 ggtcggcgga tcgtgataga gcgtctgccc atgcgccgcc accgcggcga cttcgcccgc
     2521 gctgattccc gcggcgacaa gtgtctcgtt caccgcccgc gcgtacgcca gcgaaatctg
     2581 tcgggccagc gccgccaggt ccgcgagttg aatttcgcca gcgtttcgac agccgaagat
     2641 cgcattgcgc agggcggcat cgtacgcgac atggtggtga tgaatcagcc gggccgacat
     2701 gtccaagccc gcgccatcga cgcggacgag tgcgacgtca acgccgtcgg cgctggtgcc
     2761 gctcatcgcc ccggcgatga tgcgtgatgg acgctcagcc atgggcaaga ctttaacgcg
     2821 tgcgcggcag aaggcgaacg cgacggcgcg aggtcgcgaa ggaaaacgcg aagattgcga
     2881 atgagtggtt cttcaaagtg caacgccact cgcacgggcc ggaggcccgt atcacgatag
     2941 aggtcacata tgctcaacgt gacacgggcc tccgcccgtg cgagcggcga tccgatatcc
     3001 agtgctctcg ataacaaatt cttctccctt cgcgtctgtc ttcgcgtctt cgcgctcgca
     3061 tttccgcacg cttggtgcgg attaccggaa ggcgggcatt ttctgttccg cgttggggcg
     3121 ggcgggttgt ataatccccc cgcgacgcct gcagtgggtt ggcgtccggt tggagctgtt
     3181 tatggccgag aagagcaaga agaaaacgga agatgatgcg ggcgcatcat cccagggcaa
     3241 cactggcggc ggtggtgcga cggcgaccgc gaagccgaag aaggcgatga agcgcgcgcc
     3301 caaaaagcgc ccgccgggtt tcctgccgcc gtggaaagtg ctgttgcaca acgacgacaa
     3361 gaattcggtc gatttcgtga tcgtgacgat cgtcgagctg acgccgctcg ataagcagca
     3421 ggcggagctt cgcacgaagg aagccgacaa gaccggcgtg gccctgctgt tgaccacgca
     3481 caaggagcgg gccgagcttt atcaggagca gttccagtcc aaggggctga ccgtcacgat
     3541 cgagccggcg gaagaaggat aaggctgcaa aagattccgg caagaatcta gaggaagcga
     3601 ttcaatcgct tcctttttgc tttggcgaac tcggatcgcg acgcacgaac accgccgcgg
     3661 ggtcatcacg aagggcctgc tcaatgttgc gtcgatgatc ggggcgcatc tcgggaagtt
     3721 cgtcgagctt aaaatatcga acgtccgtcg tctcatcgtc gttcacgcac ggctccccac
     3781 cgaccggccg gcagcggaag acgatcgtga cgtagtggac gcggtcgcca ttttgtacgt
     3841 cacttccggc ccatcgaaca cgccgaccaa tcgctcgggc accacctcaa cgctggtctc
     3901 ttcgtgaatc tcgcgaacaa cggcgtcggc cggctcttcg ccgggctcga tcatcccgcc
     3961 gatcggcatc cagccgagga cttccttcga tttcaccagc agcacccggc ggtcatcggt
     4021 gatgacgacg ccggacacgc cgacgaggtg cagcagatcg tggccgatct tttggcggat
     4081 gttacggatg aattcggagg ctggcatatc ccgccttgtc ctgagcgaag cacgtactcg
     4141 tgcgtagtcg aaggatctag ccgggagtcg gtgaacgctt cacttcttgg ttcacgcacg
     4201 aattcactca acggcttatg cttcaatatc tatctcgcca tcgaccgttc accgacgttc
     4261 cagctaaatc cttcgactac ggcgagtacg cctgcgctca ggacaaggca gcgtccaagg
     4321 cgctgcacac cgcgtcgcca acctgcgtcg tcgtcgcggt accacccagg tcacgcgtgt
     4381 ggttcttcgg attggcgacg acacccttgc acgcctggtt gatggcggcg gcggctttgg
     4441 gttggttcag gaaatccagc atcatcgcga cgctcaggaa cgtcgcgatc ggattggcga
     4501 aatttttgcc ggcgatatcg ggggcgctgc cgtgcaccgg ttcaaacatg ctggccgtct
     4561 gccgggtcgg attcaggttg ccacttgccg ccatgcccat gccgccctgg atggcggcgc
     4621 ccaggtcc
//
LOCUS       DATIMH010001075         1887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5170170, whole genome shotgun sequence.
ACCESSION   DATIMH010001075 DATIMH010000000
VERSION     DATIMH010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5170170"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VLI90_18590"
     CDS             complement(<1..795)
                     /locus_tag="VLI90_18590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV16278.1"
                     /translation="MIALTPVTSLATVERRDRFMRVAAANPRILVGGGIILAILAACL
                     LTLPLTRPSTSSLYYDRQDLTVVRKPPGVVPGARGWFGTDILGRSLFSRCLIGGTISL
                     GIGAASAAVSLVLGVSVGLVAGYRGGWIDSFLMRTVDVLYGLPEILMIVLLQFALRDR
                     VPNVVVLFLAIGLVSWLTMARVVRGQVLSLRGLPFIEAARAAGLPERRIFLRHIFPNL
                     AGPIIVYATLTVPRAILEESLLSFLGIGIAPPLSTWGSLAADALQPA"
     gene            complement(915..>1887)
                     /locus_tag="VLI90_18595"
     CDS             complement(915..>1887)
                     /locus_tag="VLI90_18595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV16279.1"
                     /translation="SHFALSLYHAPPMLRFLAVRLLQFPLVLAVIYLITFLLVWVAPG
                     SPFERTDRKLDPRVLEQIKVRMHAQNAWTFLAWYPRNLVLHGDFGYSLNYEEWTVNDI
                     LKSALPVSLTLGMFGLTIATFAGVALGALAAVRRGGPLDFLSLALALIGISVPSFVTA
                     AGLLVAFAVKVHWFPVGGWGSLRDLVLPGIALSLLPMAYIARLTRAAMLDILGSDYVR
                     TARAKGLSRSKVIWKHCLRNACLPVLSYLGPAAAAALTGSFVVEKVFNIPGLGQHFVN
                     SVLNRDQTLVLGTVIVYSAFLLTLNLLVDVGYAFIDPRIDIVNAKAP"
BASE COUNT          409 a          571 c          639 g          268 t
ORIGIN      
        1 cgccggttgc agcgcgtcgg cggcgagcga accccaggtg ctcagcggcg gcgcgatgcc
       61 gatgcctagg aagctaagca gcgattcttc aaggatcgcg cgcggcacgg tgagcgtggc
      121 gtacacgatg atcggcccgg caaggtttgg gaaaatgtgg cgaaggaaaa tgcggcgttc
      181 gggtaatccc gcggcgcggg ccgcttcgat gaagggcaag ccgcgcaggc tcagcacctg
      241 cccgcgcacg acgcgggcca tcgtcaacca gctgaccagg ccgatggcga gaaagagcac
      301 gacgacgttc ggcacgcggt cgcgcagggc gaactgcagc agaacgatca tgaggatttc
      361 cggcaggccg tagaggacgt cgacggtgcg catcaaaaat gaatcaatcc acccaccgcg
      421 gtaaccggcg acgaggccca cgctgacgcc taatactaat gacacggcgg cgctggccgc
      481 gccgatgccg aggctgatcg tcccgccgat caagcagcgg ctgaacaagc tgcggccgag
      541 gatgtcggtg ccgaaccacc cgcgtgcccc cggcaccacg cccggcggct tccgcacgac
      601 ggttaaatct tgtcgatcgt aatacagcga tgaagtcgaa ggccgcgtga ggggcagcgt
      661 cagaagacat gcggcgagaa tggcgaggat gatcccgccg ccgacgagga tgcgcgggtt
      721 cgccgcggcg acgcgcatga agcgatcgcg gcgttcaacg gtcgcgagcg aagttacggg
      781 cgtgagagcg atcatcgcat ccgaacgtcg aacaccccgt caagcacagc gattgcagcg
      841 ccctgtccgc cgcttgcggc ttagcatctt tcgtcgcccg aaacacatgc taagccgcaa
      901 gcggctgtga cgaattaggg cgccttcgca ttcacaatat caatccgcgg atcgatgaac
      961 gcgtatccga catccaccag taaattcagc gtcaacaaaa atgctgagta gacgatcacg
     1021 gtgccgagca ccagcgtctg gtcgcggttt aagacgctgt tgacgaagtg ctggccgagg
     1081 ccggggatat tgaacacctt ttcgacgacg aacgagccgg tcagcgccgc ggctgccgcg
     1141 gggccgaggt agctcagcac cggcaggcac gcgttgcgga ggcagtgttt ccagatcact
     1201 ttcgagcgtg acaatccttt ggcgcgcgcc gtgcgaacgt aatcgctgcc gaggatatcg
     1261 agcatcgccg cgcgcgtgag gcgcgcgatg tacgccatcg gcagcagtga cagcgcgatg
     1321 ccgggcagga ccaggtcgcg cagcgatccc catccgccga cgggaaacca gtgcaccttc
     1381 accgcaaacg cgacgagcaa cccggcggcg gtgacgaaac tgggcacgct gatgccgatc
     1441 agcgcgaggg caaggctcag gaaatccagc ggcccaccgc gtcgcaccgc ggccagcgcg
     1501 cccagcgcca cgcccgcgaa ggtggcgatc gtgaggccga acatgccgag cgtgagcgag
     1561 accggcagcg cggacttgag gatgtcgttc acggtccact cttcgtagtt caagctgtag
     1621 ccgaagtcgc cgtgcaggac gaggtttcgt ggataccagg cgaggaacgt ccacgcgttt
     1681 tgcgcgtgca tgcggacctt gatctgctcg agcacgcgcg ggtcgagctt gcgatcggtt
     1741 cgttcaaaag gagaccccgg cgcgacccag acgagcagga acgtgatcag gtaaataacc
     1801 gccagcacca ggggaaactg cagcaggcgg acggcgagga agcggagcat tgggggcgca
     1861 tggtaaaggg aaagcgcgaa atgggaa
//
LOCUS       DATIMH010001076         4451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3143994, whole genome shotgun sequence.
ACCESSION   DATIMH010001076 DATIMH010000000
VERSION     DATIMH010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3143994"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..507)
                     /locus_tag="VLI90_18600"
     CDS             complement(109..507)
                     /locus_tag="VLI90_18600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HSV16280.1"
                     /translation="MRTPRFLSVERVLALHRLQLEKFGGQEGVRDQALLESAVAMPAA
                     SFGGQFVHAGLAEMAAAYLFHINRNHPFLDGNKRTATAAAVVFLELNGVDFVASNEEL
                     TAIALAVASGTADKAAAADFFRRHIPPTQD"
     gene            complement(508..735)
                     /locus_tag="VLI90_18605"
     CDS             complement(508..735)
                     /locus_tag="VLI90_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16281.1"
                     /translation="MMKTLTKQGDRLIFEVDDPIRQLWRIDAKTPLTVTVDGDRLILE
                     AQRDEVSDAEFDAAAAKVTTRYEQTFRKLAE"
     gene            825..1943
                     /locus_tag="VLI90_18610"
     CDS             825..1943
                     /locus_tag="VLI90_18610"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV16282.1"
                     /translation="MANEDRYQGRNIRFGIVGCGAIGPTHAGAIQQIDDADLVAVADT
                     VADRARAMADKFGSPKVYADQHEVFADPNVDVVCLCTPSGMHADGAVAALRAGKHVIV
                     EKPMEISLEACDRMIAAERESGRTLTVISQHRFDPASMLVKQLIDGGKLGKIILATAE
                     VKWWRTQKYYDSGEWRGTWKFDGGGALMNQGVHTVDLLQWLTGGVASVFGQVRTSAHD
                     RIEVEDIATASLTFRSGAVGTIVASTAAYDGLPVRIDIFGTDGSAILEGDRLKLVTLK
                     TGETYASEEAAAHALSVAKGGTASVRDEAAHREAAAGQGQVWGDAHRAQIQDFIRAIR
                     TGGKPLIDARSGRDPVEIILSVYESARSGKPLTLTSKK"
     gene            2176..3453
                     /locus_tag="VLI90_18615"
     CDS             2176..3453
                     /locus_tag="VLI90_18615"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HSV16283.1"
                     /translation="MNMPKRGSYIIPLAAALVVSILGCKPPQKPPVAQQLDYAAELPP
                     GQLALRKLSPGEYPDFSQAILAFNVQDLGKSIDHSLSYLNAPSSRSFFPYLDIDHDRA
                     VASLHALKQLIQSESSGATNDGGRHFDATIKNAFEVYKSIGAPDPAGGRYTERVLFTG
                     YFTPIYNASLTRQGPFQYPLYKRPSDLVLDSTGEHASRRTPEAGMAPYYTRKQIEQDH
                     VLDGQELCWLADRFDAYVITVQGSARLRLPDGRIYEVGYNGNNGYEYTSPGRRMLADG
                     LITKDQLTLRGLRNYFAAHPDAMDKYLSLNDRYIFFTERPGGPYGSLNVPVTAFATIA
                     TDKSVYPRAMPAFLAVPVPSPSGGVTPFRGFMLDQDTGGGIRASGRCDIFMGIGQQAE
                     QLAGQELHPGELYYLAVKPELVPNYLHDTPRPE"
     gene            complement(3531..>4451)
                     /locus_tag="VLI90_18620"
     CDS             complement(3531..>4451)
                     /locus_tag="VLI90_18620"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSV16284.1"
                     /translation="VFFSPANKTTHAKPQAAHSTSMDSPLNQRRRTTQGVLILTLTTF
                     IWGTTFVVVKLTLKGFPAGALVFARFAIAAAIFTPFLRLGHKLWLAAFELGVLLWIGF
                     ATQTIGLRYTSVNRSAFITSMHVIFVPAFAGLLGRGSRPAVWIAAALALLGVGLLSHD
                     GSPPNVGDLWTAGCAIAWAIYITRLETFAQALPSKPLTAAHLWVVAVLSALWTWRSPE
                     AIGAIPWLAILYLGVAATAVTTWFQLLGQRWVSAPRAAVLYTMEPVWAALFGWIVLRE
                     RLGLTGWIGASLIILAALLTQVRSGRKQTA"
BASE COUNT          895 a         1430 c         1382 g          744 t
ORIGIN      
        1 ttacctcggc gctctcggcg cctcggcggt tgcctttgcg acgatccggc cgcgagaact
       61 gaaagaccgt gagccacaga tgcacgcgga tgaacgcaga taaggtcatc aatcctgcgt
      121 aggcggtatg tgccgccgga agaaatccgc cgccgctgcc ttgtcggcgg tgccggaagc
      181 aacggcaagc gcgatcgccg taagttcctc gttgctcgcg acaaaatcca cgccgttcaa
      241 ttccagaaac accaccgccg ccgccgttgc cgtgcgcttg tttccatcga ggaacggatg
      301 attgcgattg atgtggaaca gataggcagc cgccatttcc gccaggcccg cgtgcacgaa
      361 ttgaccgccg aatgatgcgg ctggcatcgc cactgccgat tcgagtagtg cctgatcgcg
      421 cacaccctct tgcccaccga acttttccaa ctgtaatcga tggagagcga gcacgcgctc
      481 gacggaaaga aaccgcgggg tgcgcattta ttccgccagt tttcgaaacg tctgctcgta
      541 tcgagttgtg accttggccg ccgccgcgtc aaactccgcg tcgctgactt cgtcgcgctg
      601 ggcttcgaga atcagcctgt cgccgtcaac cgtcaccgtc aacggtgtct tggcatcgat
      661 cctccagagt tggcgaatcg gatcgtccac ttcaaagatt aggcgatcgc cttgcttggt
      721 cagcgttttc atcatgaacg tcactcccgc attcattgta gccgcggttg ctacccagat
      781 gccatcattt ctgtaatatg cccggaccat caggaggccc gcgcgtggcg aacgaagacc
      841 gttatcaagg acgcaacatc cgcttcggca tcgtcggctg cggggcgatc gggcccacgc
      901 acgcgggggc gattcagcag atcgacgacg cggatctggt tgccgtcgct gacacggtgg
      961 ccgatcgcgc ccgcgcgatg gcggacaagt ttggatcgcc gaaggtttac gctgatcagc
     1021 acgaggtctt cgccgatccg aatgtcgatg tcgtctgtct ctgcacgccc agcggcatgc
     1081 acgccgacgg cgccgttgcc gctctgcgcg cgggcaagca tgtgatcgtt gaaaagccga
     1141 tggagatttc gctcgaggcg tgcgatcgca tgatcgccgc ggagcgcgaa tctggacgca
     1201 cgctcaccgt catcagccag caccgcttcg acccggcgag catgctggtc aagcagctga
     1261 tcgacggcgg aaagctcggc aagatcatcc tcgccaccgc cgaggtgaaa tggtggcgca
     1321 cgcagaaata ttacgacagc ggcgaatggc gcggcacgtg gaagttcgac ggcggcggcg
     1381 cgctgatgaa ccagggcgtg cacaccgtcg atctcctgca atggctgacc ggcggcgtgg
     1441 cgtcggtctt cggccaggtc cgcaccagcg cgcacgatcg catcgaagtg gaagacatcg
     1501 cgacggcgag cctgacgttt cgcagcggcg ccgtcggcac gatcgtcgcc agcaccgccg
     1561 cgtatgatgg gctgcccgtg cgcatcgata tcttcggcac cgacggcagc gcgatcctcg
     1621 aaggcgatcg gctaaaactc gtgacgctga agaccggcga gacctacgcc agcgaagaag
     1681 cggccgccca tgcgctcagc gtcgccaagg gcggcaccgc cagcgtgcgc gacgaagccg
     1741 cccaccgcga agccgccgcg ggacaggggc aagtctgggg cgacgcccac cgcgcgcaga
     1801 tccaggattt catccgagcc atccgcaccg gcggaaaacc gctcatcgac gcccgcagcg
     1861 ggcgcgaccc ggtggagatc atcttgagcg tttacgaatc cgcccgcagc ggaaaaccgt
     1921 taacgttgac gtccaaaaag tgaatttgaa aacgtccccg tgaaatgcaa ccgccaagac
     1981 gccgagggga ggaagagttt taaccacaga tttcgcagat gacgcagatg aacaaagagc
     2041 atcttccaat ctgcgcaatc tgcaaaatct gcggttcaga aagagtcttg tcaatgtctt
     2101 ctcggcgtct gcctcggcgt gctcggcgcc ctcggcggtt gcatttgatt caccggaaat
     2161 atctaggtcc aactcatgaa catgcccaag cgcggaagct acattatccc cctcgcggca
     2221 gcgctcgtgg tttcgatcct cggctgcaag ccgccgcaga aaccgccagt cgcgcagcag
     2281 ctcgattacg ccgccgagtt accgccgggg cagttggcgt tgcggaaact gtcgccgggg
     2341 gagtacccgg atttctcgca ggcgatcctc gcgttcaacg tgcaggacct gggcaagtcg
     2401 atcgatcaca gcctcagcta cctgaacgcg cccagcagcc ggtcgttctt cccgtacctg
     2461 gacatcgacc acgaccgcgc ggtcgcgtcg ctgcacgcgc tgaagcagtt gatccaaagc
     2521 gaatcgagcg gcgcgacgaa cgacggtggc cggcacttcg acgcgacgat caaaaatgcg
     2581 tttgaggttt acaagagcat cggcgcgccc gatccggccg gcgggcgata caccgagcgg
     2641 gtcctgttca ccggctactt cacgccgatt tacaacgcga gcctgacgcg acaggggccg
     2701 tttcagtatc cgctgtacaa gcggccgagc gacctggtgc tggacagcac cggcgagcac
     2761 gcctcgcgtc gcacgcccga ggcgggcatg gcgccatact acacgcgcaa gcagatcgaa
     2821 caggaccacg tgctggacgg gcaggaattg tgctggctgg ccgaccggtt cgacgcgtac
     2881 gtcatcaccg tgcagggttc ggcccggctg cgcctgccgg acgggcggat ttacgaagtc
     2941 ggttacaacg gcaacaacgg ctacgaatac accagccccg gcaggcgcat gctggccgac
     3001 ggattgatca ccaaggacca gctgaccctg cgcgggctgc ggaattattt cgcggcccat
     3061 ccggacgcga tggacaagta cttgtcgctg aacgaccgct acatcttctt caccgagcgc
     3121 cccggcgggc cgtacggcag cttgaatgtg ccagtcactg cgtttgctac gatcgcaacg
     3181 gacaaatccg tctacccccg cgccatgccg gcatttttag cagtgccggt gccatcaccc
     3241 agcggcggcg tgacgccgtt ccgcgggttc atgctggacc aggacaccgg cggcggcatc
     3301 cgcgccagcg gtcgatgcga tatcttcatg ggcatcgggc aacaggccga gcaactcgcc
     3361 ggacaggaac tgcatccggg ggagctgtat tacctggccg tgaaaccgga gctggtgccg
     3421 aattatttgc acgacacgcc gagacccgaa taagcggcgt gctcgcttca gcgaccatgt
     3481 cccccgcagc atgtcgcgcg aagacatggt cgccgaagcg accacgccgc ttatgcggtt
     3541 tgctttcgac ctgagcgcac ctgcgtcaac agcgccgcca ggatgatgag cgatgcaccg
     3601 atccatccag taagccccag tcgctcgcgt agcacaatcc atccaaacaa cgccgcccac
     3661 accggctcca tcgtgtacag caccgcggct cgcggggcgc tgacccagcg ctggccgagc
     3721 agttggaacc aggttgtcac cgccgtggcg gcgacgccga gatagagaat cgcgagccag
     3781 gggatcgcgc cgatcgcctc gggcgatcgc cacgtccaga gcgcgcttag aaccgcaacc
     3841 acccacaaat gggccgccgt cagcggcttg ctcggcagcg cctgcgcgaa cgtctcgagc
     3901 cgcgtgatgt agatcgccca ggcaatcgcg catccggcag tccacaagtc gccgacattc
     3961 ggcggcgagc cgtcgtggct cagcaacccg acgccgagca gcgccagtgc agcggcgatc
     4021 cacaccgccg ggcgcgatcc gcggccgagc aaaccggcga aggcgggcac gaagatcacg
     4081 tgcatcgacg tgatgaacgc gctgcggttg acgctcgtgt agcgcaggcc gatcgtttgt
     4141 gtggcgaagc cgatccacag caggacgccc agttcgaagg cggcgagcca aagcttgtgg
     4201 cccaggcgaa ggaacggcgt gaagatcgcc gcggcgatcg cgaagcgagc gaacaccagc
     4261 gcgccggcgg gaaaaccctt gagcgtcaac ttcaccacga cgaacgtcgt tccccaaatg
     4321 aacgtcgtca gcgtgaggat gagcacgccc tgcgttgtgc ggcggcgctg gtttagcggg
     4381 gaatccatcg aagtcgaatg agccgcttgc ggcttagcgt gtgttgtttt gttcgctggg
     4441 gagaaaaaga c
//
LOCUS       DATIMH010001077         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5170449, whole genome shotgun sequence.
ACCESSION   DATIMH010001077 DATIMH010000000
VERSION     DATIMH010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5170449"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VLI90_18625"
     CDS             <1..1166
                     /locus_tag="VLI90_18625"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HSV16285.1"
                     /translation="IQLAVPFCQAGLAGYSDRAMRTVARRRGCPYAVTEALLDVILLS
                     GGQGLKKSIDIGDEDHPVAGQIIGSEPDTMSRAAAILAQHGYDVIDLNFACPVKKIKN
                     KSRGGHMLMDVPRGVDILKAVRDSLPPNVPTTVSLRRAFDDEPESVDRFYELVDTAFA
                     LGFAAVRVHGRTVEQKYQGRASWPFLREVKQRYPDRAILGSGDVFTAEDAVRMLSETG
                     VDIVWIARGAIGNPWIFQHAAALLAHSSPSPGTPGEGRGEGDLDFPTASEMLNHPHPN
                     PLPDYRERGPEIGPPTIHAQRDALTEHFGIAMQIHGEQLAGRRMRKMGIKYSRFHPQS
                     ADVKHDFIEVHSLRDWNNVLSKWYASDGPGVWPSPTAANEVNADEQTCEPAVG"
     gene            complement(1177..1797)
                     /locus_tag="VLI90_18630"
     CDS             complement(1177..1797)
                     /locus_tag="VLI90_18630"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV16286.1"
                     /translation="MSSASIKPHPAMPTAEPAWDIARLFPNQGHWTVDEYLRLNGNYF
                     VEFTNGYIEVLSMPTQFHQLIVRFIFRLLLAYVDRTQAGEPLFAPLRVRLAQGKYREP
                     DIVFMLVANAHRRHNEFWEGADLVMEVVSDDDRRRDLELKRFEYARAGIPEYWIVDPQ
                     LRQITVLTLSGGEQYTEHGIFPAGERATSVILPGFEVPVTETFDVK"
     gene            1881..2354
                     /locus_tag="VLI90_18635"
     CDS             1881..2354
                     /locus_tag="VLI90_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16287.1"
                     /translation="MEADRCLLLHHLRREVLSARDRGRIVIEFADGVPVTTLTEARQM
                     ADGMIALVNLPMLGPHWYPVDRARAATILTEVLHWDLETLSARVPEQQARELTEKILS
                     QFPKDAYFLTNVRPTADLTDSPHRWASGSELAELDAGVAIVSQELVGLCWVEDRP"
     gene            2461..2730
                     /locus_tag="VLI90_18640"
     CDS             2461..2730
                     /locus_tag="VLI90_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16288.1"
                     /translation="MHSKVPPRNRLAKVLPEQWRKMLVAHGAPKRKYTAVLRATLADG
                     RVIEDLIVEQGWIIALDREGLGGTFEQRIDFDPRTIESIEILQVV"
     gene            2846..>3092
                     /locus_tag="VLI90_18645"
     CDS             2846..>3092
                     /locus_tag="VLI90_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16289.1"
                     /translation="MRTVRLAILGSSPCDQCDAACCKQNGHEFAVLLHADERRRFAAW
                     SVDLPVTSSKGIVLERVIAYRGGRCPFLGDDDRCTIYE"
BASE COUNT          557 a          948 c         1038 g          549 t
ORIGIN      
        1 cgattcaact tgccgttccc ttctgtcagg ccggcctcgc tggatatagc gatcgcgcca
       61 tgcgcacggt cgcgcgccgc cgcgggtgtc cgtatgcggt tacagaagca ctgctcgacg
      121 tgatcctgct cagcggcggg caagggctga agaagagcat cgacatcggc gacgaggacc
      181 atcccgtcgc cgggcagatc atcggcagcg agccagacac gatgtcgcgc gccgcggcga
      241 tcctcgccca gcacggctac gacgtcatcg acctgaactt cgcctgcccg gtcaagaaaa
      301 ttaaaaacaa gtcccgcggc ggacacatgc tgatggacgt gccccgcggc gtggacatcc
      361 tcaaggcggt gcgggattca cttccgccaa atgtgccgac gaccgtgagc ctgcgccgcg
      421 cgttcgatga cgagccggag tcagtcgatc gattttacga gctggtggat accgcgttcg
      481 cgctcggctt cgccgcggtg cgcgtgcacg ggcggaccgt cgagcagaaa tatcaaggcc
      541 gggccagctg gccgttcctg cgcgaggtga agcagcggta tcccgatcga gcgatcctcg
      601 gcagcggcga cgtcttcacc gccgaggacg cggtgcgcat gctgagcgaa accggcgtgg
      661 acatcgtgtg gatcgcccgc ggggcgattg ggaatccatg gattttccag cacgcggcgg
      721 cgttgttggc gcattcttcc ccctctcccg gtacaccggg agagggccgg ggtgagggtg
      781 atttagattt tccaacggct tccgaaatgc taaatcaccc tcaccctaac cctctcccgg
      841 attaccggga gaggggacca gagattgggc cgcccaccat tcacgcgcag cgcgacgccc
      901 tcacggagca cttcggcatc gcgatgcaga tccacggcga gcagctcgcc gggcggcgga
      961 tgcggaagat ggggatcaag tacagccgat tccacccgca gtcggccgac gtgaagcacg
     1021 acttcatcga ggtgcacagc ctgcgcgatt ggaacaacgt cttgagcaag tggtacgcca
     1081 gcgacggccc cggtgtctgg ccgtcaccga cggcggcgaa tgaagtgaat gccgacgagc
     1141 aaacctgcga gcccgcggtg gggtgagcgg ccgttctcac ttcacgtcga acgtttcggt
     1201 gacggggact tcgaagccgg gcaggatgac ggaggttgcc cgctcgccgg cggggaagat
     1261 gccgtgctcg gtgtattgtt cgccgccgct cagcgtcagc accgtgatct ggcgaagctg
     1321 cggatcgacg atccaatatt ccgggatgcc ggcgcgggcg tactcgaatc gtttgagttc
     1381 caaatcgcgg cggcggtcgt catcgctgac gacttccatg accaggtctg cgccttccca
     1441 gaattcgttg tggcgacggt gtgcgttggc taccagcatg aagacgatgt ccggctccct
     1501 gtattttccc tgtgccaatc gaacccgcag cggtgcaaag agcggctcac ccgcttgcgt
     1561 tcggtcaacg tacgccagca gcaggcgaaa aataaatcgg acgatcagtt ggtgaaactg
     1621 cgtcggcatc gacagcacct cgatatagcc gttggtgaac tcgacgaaat agttgccgtt
     1681 gaggcgcagg tattcgtcga ccgtccaatg cccctggttg gggaacagcc gggcgatgtc
     1741 ccacgccggc tcggcggtcg gcatggcggg gtgcggtttg atggaagcgc tgctcatacg
     1801 atggccttgt gtcctacgct ataatacgtc gggagcgagc tgttcgcatc cgaaaactgt
     1861 gcggggagcg cgagtgaccg atggaagctg atcgttgcct gctgctgcat cacctgcgac
     1921 gtgaggtgct gtccgcgcgc gatcgcgggc ggatcgtcat cgaattcgcc gacggcgtgc
     1981 cggtgacgac gttaaccgaa gcgcgccaga tggccgacgg catgatcgcg ctggtgaacc
     2041 tgccgatgct cggcccgcac tggtacccgg tggatcgcgc gcgggcggcg acgatcctga
     2101 ctgaagtgtt gcattgggac ctggagacgc tgagcgcgcg cgtgccggag cagcaggcgc
     2161 gcgagctgac ggagaagatc ctctcgcagt tcccgaagga tgcgtacttc ctaacgaacg
     2221 tgcggccgac agcggacctg acggactcgc cgcaccgttg ggcctcgggg tcggaactgg
     2281 ccgagctgga cgcgggcgtt gcgattgtct cgcaggagct ggtgggactg tgctgggtgg
     2341 aagaccggcc gtaatcgttt agccgccgcg gtctcgcggc gtttttctcc gtccgcgccg
     2401 cgaggccgcg gcggctaaac ggggcgcggc tatcgctacg ccgtgaaatg agagcgtttg
     2461 atgcattcga aagtgccccc gcgaaaccgt ctcgcaaaag tattgcccga gcagtggcgg
     2521 aagatgctcg tcgcgcacgg cgcgcccaag cgcaaataca ccgcggtgct gcgcgcgacg
     2581 ttggccgacg gccgcgtgat cgaagacctg atcgtcgagc aaggctggat catcgcgctc
     2641 gaccgcgagg gcttgggtgg cacgttcgag cagcgcatcg acttcgaccc gcgcacgatc
     2701 gagtcgatcg agatcctgca ggttgtttga gaaatcgctc gagaaccgca ggcgacagcc
     2761 agcggtggcg ctcgatatcg gactgggctt ggtgctcgaa agaccccgct ggctatcgcc
     2821 cgcggctgta aagtgtccgc tcgccatgcg aaccgtccgc ctcgcgatcc tcggctcaag
     2881 cccctgcgac caatgcgacg ccgcgtgctg taagcagaac gggcacgagt tcgcggtgct
     2941 gctgcacgcc gacgaacgcc gccgcttcgc ggcgtggtcg gtcgatctgc cggtgacatc
     3001 ttcgaaaggc atcgtgctcg agcgcgtcat cgcgtaccgc ggtggccgct gcccattcct
     3061 tggcgacgac gaccgctgca cgatttatga gg
//
LOCUS       DATIMH010001078         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2567023, whole genome shotgun sequence.
ACCESSION   DATIMH010001078 DATIMH010000000
VERSION     DATIMH010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567023"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VLI90_18650"
     CDS             <1..1415
                     /locus_tag="VLI90_18650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HSV16290.1"
                     /translation="LESPAVLQLNDPRLAILLRNWRPDVELFREENVPLETEATKLVN
                     EYDKISGAMTVEFDGKERTMQQMARYQEQVDRDLRQRAWEITVDRRLKDREPIDDIFD
                     KLLGLRQQMATNAGMGDYRALVWKAYKRFDYTPEQCLAFADAIADTCVPLVKRLDALR
                     KRDLRIDRLRPWDTAVDPHNRPPLEPFKEDQTQAFVEKTRLIFERLSPDLARDFESLR
                     TNRNLDLESRKGKQPGGYQAQLEEARQPFIFMNAAGLQRDVETLLHEGGHAFHFLAAS
                     ANEPLVFLRSAPMEFSEVASMSMELLGGEHLDVFYSEADSARARRTLYEGIIRILPWI
                     ATIDVFQHWLYTNPGHTREQRTREWLEILNRFGGDVNWSGYETYRQAMWQRQLHLFHV
                     PFYYVEYGIAQLGALQLWLKAREDPRRALANYRAALKLGGTRPLPELFAAAGIRFDFS
                     QQTLRPLIEALEEELANLPA"
     gene            1456..2046
                     /locus_tag="VLI90_18655"
     CDS             1456..2046
                     /locus_tag="VLI90_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16291.1"
                     /translation="MRNSSRPSYDERIVPTARQLEMEILESPPCRDLIFRSPPNWTAV
                     IFFATLALLHTCICVPAFFHGRWEGYMSLTFACLFTAVSIASVRFVTELVVLPIQKQV
                     RVSIGVGWFKYERQFPFSSIRGVRLTLYSDRPHGESRIELLCPGDDVQCPPTRVPREE
                     ALCLAMTMNVPLIKTFPDGVSPTATTPRRFDDVSLN"
     gene            complement(2109..2246)
                     /locus_tag="VLI90_18660"
     CDS             complement(2109..2246)
                     /locus_tag="VLI90_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16292.1"
                     /translation="MVGMESFPYVNNKIGPAAAPIKSADQDHWQRAARVLRLSNGSAI
                     G"
     gene            2691..>3914
                     /locus_tag="VLI90_18665"
     CDS             2691..>3914
                     /locus_tag="VLI90_18665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSV16293.1"
                     /translation="MPRWTLLEFVLVLGVMSFVRTRAFAQPADRPNFIFMITDDQRWD
                     ELGCVQKEMGDKGRYPWFKTPNMDRLAAEGVRFRNAFVTDSLCSPSRASFLTGQYNHV
                     NGVIDNHTPMPLDTETSGKVLGEAGYSTAYIGKFHHGKQKERPGFAYIASYLDQGYYH
                     DCPFNVNGTMTPSHGWIDDIATDYAIQYLKDHTAGADHQKPFFMVLGFKSPHDPRTPP
                     ERDKNLFSDTVWSWPTPNFDCRPPFLPTNAKTKHATPGQHVPMDRSTIDKFRCIAAAD
                     EEVGRVLAALDDLKLAENTVIVFVGDNGYYEGEHSLWDKRTAYEESLRIPLLVRWPRL
                     QHKGVTCDQIALNVDLAPTMLDLAGVPIPKQMQGRSWRPLLETGNDRNWRQSFFYEYF
                     YEHPYTFAPYTLAVRT"
BASE COUNT          764 a         1224 c         1213 g          713 t
ORIGIN      
        1 tgctggaatc gccagcggtt ttacaattga acgatccgcg cctcgccatc ctcctccgca
       61 attggcggcc ggacgtcgag ctgttccgcg aagagaatgt cccgctagag accgaggcga
      121 cgaagctggt caacgagtac gacaagatca gcggcgccat gaccgtcgag ttcgacggca
      181 aagagcgcac gatgcagcag atggcccgct atcaggagca ggtcgatcgc gacctgcggc
      241 agcgggcgtg ggagatcaca gtcgatcggc gattgaaaga tcgggagcca atcgacgaca
      301 tcttcgacaa gctgctgggc ctgcgacaac agatggcgac gaacgcgggg atgggtgatt
      361 atcgcgcgct cgtctggaag gcatataagc ggttcgacta tacgccggag cagtgtttgg
      421 cgttcgccga tgcgattgcc gacacatgcg tgccgctggt gaagcggttg gatgcactgc
      481 gcaagcgcga cctgcggatc gatcgcctgc ggccatggga caccgcggtg gatccgcaca
      541 atcgcccgcc gttggaacca ttcaaggagg accagacgca ggcattcgtc gagaagacgc
      601 ggctgatctt cgagcgtctg tcgccggacc tggcgcgcga tttcgaatcg ctgcggacga
      661 atcgcaacct cgatctggaa agtcgcaagg gcaaacagcc cggtggttat caggcgcagc
      721 tggaagaagc gcggcagcca ttcattttta tgaacgccgc gggattgcag cgcgacgtgg
      781 agactctgct gcacgaaggt gggcacgcgt ttcatttcct ggcggcatcg gcaaacgagc
      841 cgctggtgtt cctgcggtcg gcgccgatgg agttctctga ggttgccagc atgtcgatgg
      901 agctgctggg cggcgagcac ctggacgtgt tctacagcga ggccgacagc gcccgcgctc
      961 ggcggacgtt gtacgaaggg atcatccgca tcctgccgtg gatcgcgacg atcgacgtct
     1021 tccaacactg gctctacacc aaccccggcc acacgcgcga gcagcgcacg cgcgagtggc
     1081 tggagatcct gaatcgcttc ggtggcgacg tgaactggag cggctatgag acttatcgcc
     1141 aagcaatgtg gcagcgccag ctgcacctgt ttcacgtgcc gttttattac gtggagtacg
     1201 gcatcgcgca gctgggcgcg ctgcagctgt ggctgaaggc ccgcgaagac ccgcggcggg
     1261 cgctggcgaa ttatcgagcg gcactcaagc tcggcggcac gcgaccgctg cccgaactgt
     1321 tcgcggcagc ggggatacgt ttcgatttct cgcagcaaac gctgcgtccg ctgatcgaag
     1381 cgctggaaga ggaactggcg aacctaccgg cttgatcggc gtagcggtag ccccgcagac
     1441 gtggataatt ccgtcttgcg gaattcatcg cggccgtcct acgatgaacg catcgtgccg
     1501 accgcccgcc agctggaaat ggaaatcctt gaatcgccgc cctgccgcga tctgatcttc
     1561 cgctcgcccc ccaactggac tgccgtgatc ttcttcgcga cgctcgcgct gctgcacacc
     1621 tgcatctgcg tgcccgcatt tttccacggg cgatgggaag gctacatgag cctgaccttc
     1681 gcgtgcctct tcacggccgt ctcgatcgcc agcgtgcgct tcgtcaccga actggtggtg
     1741 ctgccgatcc aaaagcaggt ccgcgtgagc atcggcgtcg gctggttcaa atacgaacgg
     1801 caattcccct tctcctcgat ccgcggcgtg cgattgacgc tctacagcga tcgcccgcac
     1861 ggcgaatcgc gcatcgaact gctctgcccc ggcgacgacg tgcagtgccc gccgacgcgc
     1921 gtgccgcgcg aagaagcgct ttgcctggcg atgacgatga acgtcccgct gatcaagacg
     1981 ttcccggatg gcgtgagtcc gacggcgacc acgccgcggc gatttgatga tgtgtcgttg
     2041 aactagccgc ttgcggcttc gcaatccggg cggcgtggtt tgcgaagccg cacgcggcag
     2101 cgctttgatt agccaattgc agatccattc gacagccgca gcacgcgggc ggcgcgctgc
     2161 cagtgatcct gatcggccga tttgatcggc gccgccgcgg ggccgatctt attgttgacg
     2221 tacggaaagc tttccatgcc aaccacgaga gcgaattgcc ccaatacttc aggtccggca
     2281 aatcgctgaa ccagatgtcg aaacggtcac cgccccggct gtagaccggc atcgggcgtt
     2341 ccagtcgcgc gtatttcccg ccgatctttt ccggaaacaa caccatgttg cgattgtcgg
     2401 ggaccgacag ggacaacatc tcgaaggact aaaaatcatc ggtgaccgcc acgccgccgc
     2461 gtgggccgtg gtgggtgttg caagcgaaac agacgtagca gcgattcgcg gccggatgtg
     2521 cgttgtgtcg cggtgcgaac cgattcaagg ggacatagcg tcgctgcgtc cccgttcatc
     2581 cctgcggcaa ttactcgcac ggccttcgcg cttgacgccg cgggcacttc gccactagct
     2641 tccattcacc gtcacccaat tcgtacgcga acgcgaaagg aaaacagatc atgccgcggt
     2701 ggacattgct cgaatttgtg ttggtactgg gcgtgatgtc gttcgttcgc acgcgagcct
     2761 tcgctcagcc ggccgatcga cccaacttca tcttcatgat caccgacgat cagcgctggg
     2821 atgaactggg ctgcgtgcag aaggagatgg gcgacaaggg gcggtatccg tggttcaaaa
     2881 cgccgaacat ggatcgtctc gccgccgaag gcgtgcggtt tcgcaacgcg ttcgtcactg
     2941 attcgctttg ctcgccgagc cgcgccagtt ttctgaccgg gcagtacaac cacgtcaacg
     3001 gcgtgatcga caaccacacg ccgatgccgc tcgacaccga gacgtccggc aaagttcttg
     3061 gtgaggccgg ttattccacc gcgtacatcg gcaagttcca ccatggcaaa cagaaggaac
     3121 ggccgggctt cgcgtacatc gctagctacc tcgatcaggg ctattaccat gattgtccgt
     3181 tcaacgtgaa cggcacgatg acgccaagcc atggctggat cgacgacatc gcgaccgact
     3241 acgcgatcca gtacctgaag gaccacaccg ccggcgccga tcaccagaag ccgttcttca
     3301 tggtgctggg tttcaagagc ccgcacgacc cgcgcacgcc gcccgaacgc gacaaaaatc
     3361 tcttctccga caccgtctgg tcctggccga ctccgaactt cgattgccgc ccgccgttcc
     3421 tgccgaccaa cgccaagacc aagcacgcga cgccgggcca gcacgtgccg atggaccggt
     3481 cgacgatcga caagttccgc tgcatcgccg ccgccgacga agaggtgggc cgcgtgctcg
     3541 ccgcgctgga cgatctgaag ctggcggaaa acacggtcat cgtgttcgtc ggcgacaacg
     3601 gttactacga gggcgagcac tcgctgtggg acaagcgcac cgcgtacgag gagagcctgc
     3661 gcatcccgct gctggtccgc tggccgcggc ttcagcacaa gggcgtgacg tgcgatcaga
     3721 tcgcgcttaa cgtggacctg gcgccaacga tgctcgacct ggcgggcgtg ccgatcccca
     3781 agcagatgca gggccgcagc tggcgaccgc tgctcgaaac gggcaacgat cgaaactggc
     3841 ggcagtcgtt cttctacgag tatttttacg aacacccata cacgttcgcg ccctacaccc
     3901 tggcggtgcg gacg
//
LOCUS       DATIMH010001079         1747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2567148, whole genome shotgun sequence.
ACCESSION   DATIMH010001079 DATIMH010000000
VERSION     DATIMH010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567148"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..>1747
                     /locus_tag="VLI90_18670"
     CDS             271..>1747
                     /locus_tag="VLI90_18670"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSV16294.1"
                     /translation="MARQDLPPKTSRLLMLASIILVIASLYLAQEVLVPLALATLFSF
                     LLAPLVYRLERLKAPRGVAVLVTVLFAFTILGGIGYVVYNQMDDLARRLPQYRENIQT
                     KVNSIRSGGGFFKNVKKAGEEVQHILAPPATAPSTQATQPTAAERSGQSPVPVVVTNR
                     PPEPPSEAPAPPAGQKQSPFGLISDIGGTIFGVVGTAFIVIIFTIFMLLQREDLRDRL
                     IRLIGRGQLTVTTEALDDAAARVSRYLLMQSIINGGVGVTVMVMLWTIGKWNGLPFPS
                     PALFGLLSGLLRFVPYVGIWVAAAFPLLLSLAVYSTAKVALETLGAYLLMEVIAANVF
                     EPLLFGSSTGITTIAVLVAAAFWTWLWGPVGLILSTPMTVCVVVIGKYVPGLSFLNIL
                     LADEPALAPPDRVYQRLLATDEEEAERLVDEYARTMPLEELYDTVLVPALGMAERDRH
                     AGNLDEERHTQIRRGMREIIEDLGDRHRTEVLRLAAQKTVDQ"
BASE COUNT          306 a          537 c          591 g          313 t
ORIGIN      
        1 gacgcccatg ctacttttcc cttggcgcag atcttcagcg acagcgcgga atcacacgcg
       61 ccgcagcggc acgagcgctc gcaagcgggc gtcgcggaag aagaggccta gccacacgag
      121 caccgcgaag gcgaccggca gccagaacgg ctccgacgcg tgcacgtgcg ttgcgaccgc
      181 cccgccaagg cagcgacgca gctacaagag cattttgcgc actgtgcatt cggtcgttgc
      241 cgcgtacatt agcggcaggc gcgaccacac atggcccggc aagatctccc gcctaaaact
      301 tcgcgcttgc tgatgctggc gtcgatcatc ctggtgatcg cgtcgctgta tctcgcgcag
      361 gaagtgctcg tccccttagc cttggcaacg ctctttagct tcctgctggc accgctggtt
      421 taccggctgg aacgattgaa agccccccgc ggcgtcgcgg tgctggtgac cgttctgttc
      481 gcgttcacga tcctcggcgg catcgggtac gtcgtctaca accagatgga cgacctggcc
      541 cggcggctgc cgcaataccg cgagaacatc cagaccaagg tgaactcgat tcgcagcggc
      601 ggtggattct tcaagaacgt gaagaaggcg ggcgaagagg tgcagcacat cctcgcgcca
      661 ccggcgaccg cgccatcgac gcaggcgaca caaccgaccg ccgcggaacg ttcaggccaa
      721 tcaccggtgc cggtggtggt gaccaatcgc ccaccggaac cgcctagcga agcgccggct
      781 cctccggcgg ggcagaagca gtcgccgttc ggattgatca gcgacatcgg cggcaccatc
      841 ttcggcgtcg tcggcaccgc gttcatcgtc atcatcttca cgatcttcat gttgctgcag
      901 cgcgaagacc tgcgcgaccg gctgatccgc ctgatcggcc gggggcagtt gaccgtgacc
      961 accgaagcgc tggacgacgc cgccgcgcgc gtcagccgat atctgctgat gcagtcgatc
     1021 atcaatggcg gggtgggcgt cacggtgatg gtgatgctct ggacgatcgg caagtggaac
     1081 gggttgccgt ttccgagccc ggcccttttt ggattgctgt cgggactgct gcggttcgtc
     1141 ccgtacgtcg gcatttgggt ggcggcggcg ttcccattgc tgctgtcgct ggcggtgtat
     1201 tcgacggcga aggtggcgct ggaaacgctg ggggcttatc ttctgatgga ggtcatcgcg
     1261 gcgaacgtgt tcgagccgtt gctgttcggt tccagcaccg gcatcacgac gatcgcggtg
     1321 ctggtggcgg cggcgttttg gacgtggctg tggggaccgg tgggattgat cctgtcgacg
     1381 ccgatgaccg tctgcgtcgt cgtcatcgga aaatacgttc ccggcttgag cttcctgaac
     1441 atactgctgg cggacgaacc ggcgctggcg ccgcccgatc gtgtttatca acggctgctg
     1501 gcgacggacg aggaggaagc cgagcggctg gtcgatgaat acgcgcgcac gatgccgctg
     1561 gaagagcttt acgacacggt gctggtgccg gcgctgggga tggccgagcg cgaccgacac
     1621 gcgggcaacc tggacgaaga gcggcacacg cagatccgcc gggggatgcg cgagatcatc
     1681 gaagacctgg gcgatcggca tcgcacggaa gtgctgcggc tggcggcgca aaagacggtg
     1741 gaccagg
//
LOCUS       DATIMH010001080         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_836731, whole genome shotgun sequence.
ACCESSION   DATIMH010001080 DATIMH010000000
VERSION     DATIMH010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836731"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..568)
                     /locus_tag="VLI90_18675"
     CDS             complement(86..568)
                     /locus_tag="VLI90_18675"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSV16295.1"
                     /translation="MDSTNPVDRAPADPLSSTALSIPAAITSPSASVAEALDDREFDD
                     DGDPHRHRLSEQQQLALDWLMNGGKITEAAELVGVQRRTVSRWVNHHHAFKCLYDDWQ
                     EHLRTGLEARVMSLAEAAMDNLTQAIRTEQDVKASQFILKLLGLTSQRRRRRQVPFEK
                     "
     gene            905..2854
                     /locus_tag="VLI90_18680"
     CDS             905..2854
                     /locus_tag="VLI90_18680"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSV16296.1"
                     /translation="MTHDRLIAALVQSKNEAADDALLEALRLGNEPEQAVALDALFQR
                     KTVHGLSGVIGQFEELPEPRQAEILGNIKLLHHALRAAGRSERTELRLAAMRLIALGR
                     QGKLAYVLSENLHEPEEARSKAAVEALVALARWVATETRKLQKGVGMSEYDSDTPAPP
                     HTDTLSCTYFELLDQRPEIEQAVARAIDGHRGTHGTDLLRAALLLCDWPGSKTLAILN
                     TAKHGGQSPMVRRLQQPPASEQVDAFLLGASHGGLRSHFGIAFSHIEEAPVLDALLRK
                     THWLKDHALQNCVHFVTRGTWWNDGELIRDVSRRDPGDAARIGEWLAVSGVHDVVQDE
                     RMDRLRTHAADDFGARLRLLRIAARRKRGTSVQFIKALLTDSDERIARMAAREIVRRR
                     PVDFENVLLQQMTTAPDSVRRIIGRVVGQSGFDQFWTKFDRLDRSTRKQAGRAMLKLL
                     PDAVQRLSRRLLAGAPEQRLKAMQIVQELKLADAVQDQLLKLCDDPHPRLRSKAVSVL
                     GEIPLVAPDPLIERLLVDTDPRVRANTIEVLESRHRGEFLPILIQRARVGSNRERANA
                     IKVMHRLKVSTFGAALQGMLQDPRPEHRISAMWALRQTGWWHMIGEVGRMAKGDDNLR
                     VRRYALTVLKAVSEMVREQRAKKAG"
     gene            2878..>3119
                     /locus_tag="VLI90_18685"
     CDS             2878..>3119
                     /locus_tag="VLI90_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16297.1"
                     /translation="MRNAERTSAARCAGARLLLLTCAFCILTSAIPALASPTQEEVFK
                     SINENVGESVDGTKVLAIAAAAAGITIILVLFNRRQT"
BASE COUNT          574 a          951 c         1064 g          530 t
ORIGIN      
        1 gacccggatc ctgcggcgcg ggaaaagatc cgggtcgtcg cgagtacgct ccaaacccgg
       61 cgtggggtga ttcgccaaac gcctatcatt tttcgaatgg aacctgccgc ctgcgccgcc
      121 gctgcgacgt gaggcccagc agcttcagga tgaattgcga ggccttcacg tcctgctcgg
      181 tgcgaatggc ttgggtcagg ttgtccatcg ccgcctcggc caggctcatc acgcgggctt
      241 ccaggccggt gcgcaggtgt tcctgccaat cgtcgtacag gcatttgaac gcgtggtgat
      301 ggttgaccca tcggctgacg gtgcggcgtt ggacgccgac cagctcggcg gcctcggtga
      361 tcttgccgcc gttcatcaac cagtccagcg ccagctgctg ctgctcgctg aggcggtgcc
      421 gatgcgggtc accatcgtcg tcgaattcgc gatcatcgag ggcttcggcg acgctcgccg
      481 atggcgacgt gatggcggcg ggaatggaaa gcgcggtgga cgaaagcggg tccgcgggcg
      541 cacgatcgac ggggttcgtc gagtccatgg aagctccttg gtttgggagg gagaagggga
      601 cggaaaaagt atgtccctat atctattagg tccgaaacgt atcatatttg ctctatttca
      661 tggagctaag gctcggtcgg acactcaatt gcgaataata attacgccat ggcgcgaact
      721 ggcgatgaaa ttcccaccgc tggatttcac tgtgcgcgcg ctgagacatt tcgagacatt
      781 ttcgggaaaa tgtcccgctc gcgaccaaaa tgtctcgctt ttgaaaagtg cgcgcgctcc
      841 ggcggtatga ggcgcgatcg gtcgcctttc aagtcgcctt gccgccacgt ccgataacga
      901 ggaagtgacg cacgaccgtc tcatcgctgc ccttgtccaa tcgaaaaacg aggcggccga
      961 cgatgcgctg ctggaagccc tgcggctggg gaacgagccg gagcaagcgg tggcgctgga
     1021 cgcgttgttc cagcggaaga cggtgcacgg gttgtccggc gtgatcggac agtttgaaga
     1081 gctgcccgag ccgcggcagg cggagatcct tggcaacatc aagctgctgc accacgccct
     1141 tcgagccgcg gggcggagcg agcgaaccga gcttcggctg gcggcgatgc gcctgatcgc
     1201 gctcggccgg cagggcaagc tggcgtacgt cctgtcggaa aacctgcacg agccggaaga
     1261 ggcccgcagc aaggcggcgg tcgaggcgct ggtcgcattg gcgcgatggg tggcgacgga
     1321 gaccaggaag ctgcagaaag gtgtgggaat gtcggagtat gactccgaca cccccgcacc
     1381 cccacacacc gacaccctct catgcacgta cttcgagctc ctcgaccaac gcccggagat
     1441 cgagcaagcc gtcgctcgcg cgatcgacgg gcatcgcggc acgcacggca ccgacctgct
     1501 ccgcgcagcg ctgctgctgt gcgactggcc cggcagcaag acgctggcga tcctgaacac
     1561 cgccaagcat ggcgggcaga gcccgatggt ccgccggctg caacaaccgc cggccagcga
     1621 gcaggtcgat gcgtttctcc tcggggcatc gcacggcgga ctgcggtcgc atttcggcat
     1681 cgcgttctcg cacatcgaag aagccccggt gctcgatgcg ctgctgcgca agacgcattg
     1741 gctgaaggac cacgccctgc agaactgcgt gcacttcgtg acccgcggca cctggtggaa
     1801 cgacggtgaa ctgattcgcg acgtctcccg ccgcgacccc ggcgatgcgg cgcgaatcgg
     1861 tgaatggctc gcggtgtccg gcgtccatga cgtcgtgcag gatgaacgga tggatcgact
     1921 ccggacgcac gccgccgacg atttcggcgc gcgcctgcgg ctgctgcgca tcgcggcgcg
     1981 gcggaaacgc ggcacgtccg tgcagttcat caaggcgctg ttgaccgatt ccgacgagcg
     2041 catcgcgcgg atggcggcac gcgagatcgt ccgccggcga ccggtggatt ttgaaaacgt
     2101 gctgctgcag cagatgacga cggcgccgga ttcggtccgg cggatcatcg gccgggtcgt
     2161 cgggcaaagc gggttcgatc agttctggac gaagttcgac cggctcgatc gcagcacgcg
     2221 caagcaagcc gggcgcgcca tgctcaagct cttgcccgat gcggtgcagc gattgtcgcg
     2281 gcgcttgctc gccggcgcgc ccgagcagcg gctgaaggcg atgcagatcg tgcaggagct
     2341 gaagctggcg gacgccgtgc aggatcaatt gctcaagctg tgcgacgacc cgcacccgcg
     2401 gttgcgcagc aaggcggtca gcgtgctggg cgagatcccg ctggtcgcgc cggatccgct
     2461 gatcgaacgg ctgctggtgg acaccgaccc gcgcgtgcgc gcgaacacca ttgaagtcct
     2521 ggagagccgc caccgcgggg agtttttgcc gatcctgatc cagcgcgccc gcgtgggcag
     2581 caatcgtgag cgcgccaatg cgatcaaggt gatgcatcgc ttgaaggttt cgacgttcgg
     2641 cgccgcgctt caaggcatgc tgcaggaccc gcggccggag catcgcatca gcgcgatgtg
     2701 ggcgcttcgc caaaccggct ggtggcacat gatcggcgaa gtcgggcgga tggccaaagg
     2761 cgacgacaac ctgcgcgtgc ggcgatacgc cttgaccgtg ctgaaggccg tgtcggaaat
     2821 ggtgcgcgag cagcgggcga agaaagcggg atgagttaca tgaatgcaga atgcagaatg
     2881 cgaaatgcag aacgaacaag tgcggctcgc tgcgccggcg cgcggctgct gcttctgaca
     2941 tgtgcgttct gcattctcac ttctgcaatt cccgccctcg ccagtcccac gcaggaagaa
     3001 gttttcaaga gcatcaacga aaacgtcggc gagtcggtgg atggcaccaa ggtgctggcg
     3061 atcgcggcgg cggcggcggg gatcacgatc atcctggtgc tgttcaaccg ccggcagac
//
LOCUS       DATIMH010001081         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1415167, whole genome shotgun sequence.
ACCESSION   DATIMH010001081 DATIMH010000000
VERSION     DATIMH010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415167"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VLI90_18690"
     CDS             complement(<1..241)
                     /locus_tag="VLI90_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16298.1"
                     /translation="MRWIAIIQAVYFAVTGVWPIVHIKSFIAVTGPKTDIWLVRTVGL
                     LITVVGVVIGVAGWRGEINLPIVLLATGSAAALAGV"
     gene            complement(275..1174)
                     /gene="nadC"
                     /locus_tag="VLI90_18695"
     CDS             complement(275..1174)
                     /gene="nadC"
                     /locus_tag="VLI90_18695"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HSV16299.1"
                     /translation="MYPFPTYDELRELIQIAKREDLRDDDVTSRLLIPEDKVGVGTLV
                     QKEVGIACGLPLVEMICRTYDERLRVEQIPGFHMEIIEGRFSDARVTPLLRLRGPLRS
                     LLSAERVILNFLQHMGGVATQTQRFVRRVAGTGAKIYDTRKTIPGLRALDKYAVRCGG
                     GFNLRIGLYDGLLVKDNHLAAIPLKELAGFLAPIVQRSRAETNDRLIEIEVDTLEQLR
                     EVLKVDGVHVILLDNMDCPKMEQAVELRNKAGKKGAVELEASGGVTLDTVRQIAATGV
                     ERIAVGAITHSAPALDVGLDVED"
     gene            complement(1491..>2635)
                     /locus_tag="VLI90_18700"
     CDS             complement(1491..>2635)
                     /locus_tag="VLI90_18700"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HSV16300.1"
                     /translation="KPGEKPYTLIDYFPKDYLVIIDESHVTLPQIKAMYNGDRVRKEV
                     LVDHGFRLPSAMDNRPLKYEEFEQMVSQCIYVSATPGKIELEQTGGEVVEQVIRPTGL
                     IDPEIIVHPAQGQVPHLLAQIKERVERGERVLVTTLTKRLAEDLSAYIQEAGFRGRYL
                     HSEIQTLERVEILTDLRRGDFDVLIGINLLREGLDLPEVSLVCILDADKAGFLRSETS
                     LIQTIGRAARNVNSKVILYADKITPAMQKAMDETLRRRGVQLKYNAEHGITPVTVQKS
                     IRSSLESEVKARRTVAAAVKENEQELDQTEIVRLLEEEMIEAARNLEFERAAQLRDKV
                     NEMKGAPTIKSGSGVVFAEGSGAEAREQKIWQPKSKGRPNSRRVAK"
BASE COUNT          429 a          874 c          779 g          553 t
ORIGIN      
        1 ccacgcccgc gagcgctgcg gcgctgccgg tggcgagcag gacgatcggc aggttgatct
       61 cgccgcgcca gcctgcgacc ccgatgacga cgccgacgac ggtgatcagc agcccaacgg
      121 tgcggacgag ccaaatatcg gtcttcggtc cggtcacggc gatgaacgac ttaatgtgaa
      181 cgatcggcca aacgccggtg acggcgaagt aaaccgcctg aatgatcgcg atccaccgca
      241 tgcgggctgt tatgacgcgg gtgaatcgaa atcgtcaatc ttcaacatcc aaaccgacgt
      301 ccagcgccgg cgccgaatgt gtgatcgccc cgacggcgat gcgttccacg cccgtcgccg
      361 cgatttggcg gacggtgtcc agcgtcacac caccgctggc ttccagctcg accgcgccct
      421 ttttgccggc cttgttgcgc agctcgacgg cctgctccat cttcgggcag tccatgttgt
      481 ccagcaggat gacgtgcacg ccatccacct tcagcacctc gcgcagctgc tcgagcgtat
      541 cgacttcgat ctcgatcagg cggtcgttgg tctccgcccg gctgcgctgg acgatgggcg
      601 cgaggaagcc ggccagctcc ttcagcggaa tcgccgccag gtggttgtcc ttgacgagca
      661 gaccgtcgta cagcccgatg cgaaggttga acccaccgcc gcagcgcacg gcgtatttgt
      721 cgagcgcgcg caagccggga atcgtcttgc gcgtgtcgta aatcttcgca cccgtcccgg
      781 cgactcgccg cacgaagcgc tgcgtctgcg ttgccacgcc gcccatgtgt tgcaggaagt
      841 tcaggatgac gcgctcggcc gacagcagcg agcgcagcgg cccgcgcagc cgcagcagcg
      901 gcgtcacccg cgcgtcgctg aatcggcctt cgatgatctc catgtgaaaa ccgggaatct
      961 gctcgacgcg cagccgctcg tcgtacgtcc ggcagatcat ctccaccagc ggcaacccgc
     1021 aggcgatgcc gacttccttc tgcaccagcg tgccgacgcc aaccttgtcc tcggggatca
     1081 acaggcggct ggtgacatcg tcatcgcgca ggtcttcgcg cttggcgatc tggatcaact
     1141 cccggagctc gtcgtaggtg gggaaggggt acatcgtagt ttctcctgct gcctgattat
     1201 atcgtgctgg cgagccatct gatgctcctc gcggattcat caagcggttg acagcagggt
     1261 cattcgaata aaaagtgccg tccggccgaa cgcttaccgc acgagcctta aagttgcgac
     1321 gaaggtactc ctgatattct tcgggagagc ttcgcagccg gtcccaccac tccgcctgaa
     1381 gcttctccat tgccttcatg cgttcggctg gcgtcatctg cagccactgc tgctgcctcg
     1441 tcagttttgg ttgagcttct tcttcgccca tcgcgttctc cgatggattg ctacttcgcc
     1501 acccgccggc tgttcggccg gcctttgctc ttgggttgcc agatcttctg ctcgcgcgct
     1561 tcggcgccgc tgccttcggc gaaaaccacg ccgctgccgc tcttgatcgt cggcgccccc
     1621 ttcatctcgt tgaccttgtc gcgcagctgg gcggctcgtt cgaattccag gttccgggcg
     1681 gcctcgatca tctcctcttc cagcagccgg acgatctccg tctgatccaa ctcctgctcg
     1741 ttctccttca ccgcggcggc gacggtgcgg cgagccttca cttcgctttc caggctcgat
     1801 cgaatcgact tctgcacggt gacaggcgtg atgccgtgct cggcgttgta cttcagctgg
     1861 accccgcggc ggcggagcgt ttcgtccatc gccttctgca tcgccggcgt gatcttgtcg
     1921 gcgtataaaa tgaccttgct gttcacgttt cgcgcggccc ggccgatcgt ctggatcagc
     1981 gatgtctcgc tgcgcaggaa gccggcttta tccgcgtcca gaatgcagac cagcgacact
     2041 tccggcaggt ccaagccttc gcgcagcagg ttgatgccga tcaacacgtc gaagtccccg
     2101 cggcgaagat cggtcaggat ctccacgcgc tccagcgtct ggatttcgct gtgcagatag
     2161 cgtccgcgga agccggcttc ctgaatgtac gcagacaaat cttcagccag tcgtttggtc
     2221 aaggtggtga ccagcacgcg ctcgccgcgc tcgacgcgtt ccttgatctg cgcgagcaag
     2281 tgcggcacct ggccctgcgc gggatgcacg atgatctcgg ggtcgatcaa gccggtgggg
     2341 cggatcacct gctcgacgac ttcgccgccg gtttgttcca gctcgatctt gcccggcgtg
     2401 gccgagacgt agatgcactg gctgaccatc tgctcgaact cttcgtattt cagaggccga
     2461 ttgtccatcg cgctgggcag gcggaagccg tggtccacca gcacctcttt gcgcacgcgg
     2521 tcgccgttgt acatcgcctt gatctgcggc agcgtcacgt gcgattcatc aatgataacc
     2581 aggtaatcct tcgggaaata atcgatcagc gtgtacggtt tctcgcccgg ctttc
//
LOCUS       DATIMH010001082         7054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4300887, whole genome shotgun sequence.
ACCESSION   DATIMH010001082 DATIMH010000000
VERSION     DATIMH010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4300887"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2728)
                     /locus_tag="VLI90_18705"
     CDS             complement(<1..2728)
                     /locus_tag="VLI90_18705"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSV16301.1"
                     /translation="MGSETSAGGGGPVTRLAVLLFSDIVASTELKTKFGDVEYKRLLD
                     RHNELFESSLGQISGARIIKHTGDGFLAELRTPSDAVRFALLFHSRMHLEPWQPVKLQ
                     TRIGIHQGEFTATIHAGQADVVGAAVDAAARIMSLAVGGQILLTQHVFNDARQFVRES
                     PQALPGSPPLRWLSHGPYLFRGIDEPIRVCEVGIEGISPLSPPTSDKAKRVVPHDEEQ
                     TLGWRPAVGLDVPGRVSWKLESKLGEGGFGEVWLGQHEKLKERRVFKFCFDADRLRSL
                     KREYTLFRLLREALGDREDIARLYEVKLDKPPFFLESEYTQGGNLLEWAARKRGIAAV
                     PLAQRLDIVARICEAAGAAHSVGVLHKDIKPSNILIHEEANGAVLPRLSDFGIGMLGD
                     RDSLRAHAITETGFTQLTQNSDSSRTGTRMYAPPESLLNRPFTTQGDVYALGVLLFQM
                     AVADLGRPLAPGWERDIDDAVLRQDISKAVDGDPNQRLASAAELAGRVRTLDDRRQAR
                     VAQMQQAARALARARTRRLAGIAAVGILFIAIISIAAFLRERRQADEQAHLRALAESL
                     QQSESAQHQHAQANLLRARKEIADMVAILEGKRASTDAATQPTTGVNPAGSPEDRPMS
                     LRLADSIVANYLLFQKESADPEITESLAQAYISKAGIYFGTYTAKIEEHLQWSNEALT
                     AANAGCIVLRRLIESDPARHDKYCGELADGLTEIAQFASDQNELERAYSEAVELRDDL
                     AKSGKPADAIALVDLLVKGVRTDQEKTVAQKRLNHAIDLLRAIGATSESSEQRGRLAL
                     ALARLAEINTDDSAARIGYLRSAADALMPAKAPMKFSDEELEMARLAITLQRDLAEAL
                     AAANQRVEALNCAARAAGIGDAIIKDHADWARSLILELASVQTSLAVLHATGN"
     gene            complement(2745..2975)
                     /locus_tag="VLI90_18710"
     CDS             complement(2745..2975)
                     /locus_tag="VLI90_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16302.1"
                     /translation="MRGAKQTLDETFLEIRWRCLSLAADLDRVEKNAGGADVLNADPR
                     IQKLRQAIHILLDAHLDRAERVQMLFSDTTEH"
     gene            complement(4160..4516)
                     /locus_tag="VLI90_18715"
     CDS             complement(4160..4516)
                     /locus_tag="VLI90_18715"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV16303.1"
                     /translation="MADVQTLHLAGKEFVVIERREYERLARANGGDSLEAGLPPLPAP
                     DEEGNVPAVAYGRALLARKLVLARRRAGLTQAELARRAKIRVETLNRLEKGRHNPDES
                     TFNKIEAALKAAGVRV"
     gene            complement(4518..4772)
                     /locus_tag="VLI90_18720"
     CDS             complement(4518..4772)
                     /locus_tag="VLI90_18720"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSV16304.1"
                     /translation="MANVVLTPAAQEQMNRLPGGMLRRINDVFFRLEKWPEVSGAKPM
                     RKELVGHFRIRSGAWRVLFRVKGETVIVWQIDNRRDVYDN"
     gene            complement(4856..6991)
                     /locus_tag="VLI90_18725"
     CDS             complement(4856..6991)
                     /locus_tag="VLI90_18725"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HSV16305.1"
                     /translation="MKRLLVIFSSVALLAAVAHGPLFSRAEDAVAIAPATQPTTAPST
                     QPLYKVVRGHEGFWRIVQDQSGAWWFLSPADKADFLNTVTTVQPFQKGRDVNGPSFVS
                     TDWNGGATATGDLDRWAQATIARVHDTGFKGLGAWCHPVFHKYDIPITRDLNVWDWIA
                     SGAKRLYSPDWAPAAEAAIKAQCDVLRDNKNLVGYYTDNELDWGDGSSGPSTYFDHLP
                     KNDPNRAEVLRVIQTVWTTPEQFNKDWRARISDWRDVENWDHLPKELEPKAYTRLFTA
                     WLSHLAADYFRITTTLVHKYDPNHLVLGVRFRGYAPPEVVRAAAGYTDAQSLNYYVGD
                     ARLDLDQFRMMYETGNQPVIISEYSFHSLDGRSGDRNLVGFNAQVPDQQARADGYRLM
                     TTHLARLPYVIGADWFQWMDEPPSGRSSDGEDVNFGIVDIDDRPYPALADAVRATGPL
                     LNGLHANSAKDDQQDVWRESFATKPVAHVPFLTKPLNINGELSDWPPEAKLPAVRHSQ
                     TIGLDRSQLPLPNVYLAWSNDGLYVGLEVFDNDIEGAPAKGWWWTKDNVEFWISTKPV
                     ASDQNTYDVNCHQFFFVPNDLTATNGVGGVVGQWHREGDAINDNQIPHPDIRSATRIL
                     PDRYVVEMLIPAKALHGWDPKTQPAMAFNLHIRNFQHAIDYFWSAPKEVMTQFRPNTW
                     GPMYLEPPPAAQTAQARLAKPAPTMASSN"
BASE COUNT         1188 a         2195 c         2265 g         1406 t
ORIGIN      
        1 cgttgccggt ggcgtggagc acggccaacg acgtctgcac agaagcaagc tcgaggatca
       61 gcgagcgcgc ccagtcggca tgatccttga tgatggcatc gccgatgccg gcggcgcggg
      121 cggcgcagtt caaggcctcg acgcgctgat tggcggcggc gagggcttcg gcaaggtcgc
      181 gctggagggt gattgccagc cgcgccatct caagctcctc gtcgctgaac ttcatcggcg
      241 ccttcgccgg catcagcgcg tcggcggcgg agcggagata accgatcctg gcggcgctgt
      301 cgtcggtgtt gatctccgcc agcctcgcca gcgccaaggc cagccggccg cgctgctcgc
      361 tggactcgct ggttgcaccg atcgcccgca gcaggtcgat ggcgtggttc aaccgcttct
      421 gcgcaaccgt cttttcctgg tcagtgcgga cccctttcac cagcaggtcc accagcgcga
      481 tcgcatccgc gggtttgccg gacttcgcca gatcgtcccg cagctcaacc gcttcgctgt
      541 acgcccgttc tagttcgttc tgatcggatg cgaattgggc gatctcggtc agaccgtcgg
      601 cgagttcacc gcaatatttg tcgtggcgcg ccgggtccga ttcgatgagc cgccgaagga
      661 ctatgcatcc ggcattggcc gcagtgagcg cttcattcga ccattgcaga tgctcttcaa
      721 tcttggcggt gtaggttcca aaatagatcc ctgccttgct gatatacgcc tgtgccagcg
      781 attcggtgat ctcggggtcg gccgattcct tctgaaagag cagataattg gcgacgatcg
      841 agtcggcgag gcgcaggctc atgggacggt cttcgggcga ccccgccggg ttgacaccgg
      901 tggtgggttg cgtggcggcg tcggtcgagg cccgcttgcc ttcgaggatc gcgaccatgt
      961 cggcgatctc cttgcgggcg cgaagcaggt tggcctgggc gtgctgatgc tgggcgcttt
     1021 cgctctgctg aagggattcg gccaaggcgc gcaggtgggc ctgctcgtcg gcctgccgcc
     1081 gctcgcgcag aaatgccgcg atggagatga tggcgatgaa caggatgccg accgctgcga
     1141 tccccgccag tcgccgggtc cgagcccgtg cgagcgcgcg agcggcctgc tgcatctgcg
     1201 ccacccgcgc ctggcggcga tcgtcgagcg tccgaactcg cccagctaat tccgcggcgc
     1261 tggccagacg ctggttcgga tcaccatcca ccgctttgga gatatcctgg cgcagcactg
     1321 catcgtcgat gtcgcgctcc caacctggcg ccagcgggcg acccaggtcg gcgacggcca
     1381 tctggaacag cagcacgccc agcgcgtaca cgtctccctg cgtggtgaac gggcggttca
     1441 gcaggctttc cggcggggcg tacatccgcg tgccggtgcg gctcgaatcg ctgttctgcg
     1501 tcagctgcgt gaaacccgtt tcggtgatcg cgtgcgcccg caggctgtcg cggtcgccga
     1561 gcataccgat accgaaatcc gaaagccgcg gcagcacggc gccgttggct tcctcatgga
     1621 tgaggatgtt gctcggcttg atgtccttgt gcagcacgcc gacgctgtgc gccgcccccg
     1681 ccgcttcgca gatgcgggcg acgatgtcca accgctgcgc cagcggcacc gccgcgatcc
     1741 cgcgtttgcg ggcggcccat tccagcaggt ttccgccctg ggtgtattcg ctctccagaa
     1801 agaacggcgg cttgtcgagc ttcacttcgt acagccgggc gatgtcctcg cggtcgccca
     1861 gcgcttcgcg cagcaggcga aacagcgtgt actcgcgctt gaggctgcgc agccgatccg
     1921 catcaaaaca aaacttgaag acgcggcgct ccttcagctt ttcatgttgc ccgagccaaa
     1981 cctcgccaaa cccgccttcg ccgagcttcg actccagctt ccaactgact cgccccggca
     2041 cgtccaaacc caccgccggc cgccagccca gcgtctgttc ctcatcgtgc gggacgacgc
     2101 gcttggcctt atcgctagtc ggcggcgaca gtgggctgat gccctcgatc cccacttcgc
     2161 acacgcgaat cggttcatcg attccccgga acagatatgg cccgtgcgac agccatcgca
     2221 gcggcggcga tcccggcagc gcctgcgggg attcacgcac gaactgccgg gcgtcattga
     2281 agacgtgctg cgtcagcaga atctgcccac caacggccag cgacatgatg cgcgcggccg
     2341 catcgacggc ggcgccgaca acgtcggctt gcccggcgtg gatggtcgcg gtgaattccc
     2401 cctgatggat gccgatgcgc gtctgcaact tcaccggctg ccacggctcc aggtgcatcc
     2461 ggctgtgaaa cagcagcgcg aaccgcaccg catcgctggg agtgcgtaac tcggccagaa
     2521 aaccgtcgcc cgtgtgcttg atgatccgcg cgcccgagat ctggccgagg ctggattcga
     2581 aaagttcgtt gtgccggtcg agcagtcgtt tgtactcgac gtcgccgaac ttcgttttca
     2641 gctcggtgga agcgacgatg tcgctgaaga gaagtaccgc caggcgcgtg acgggcccac
     2701 cgccgccggc cgaagtctcg ctccccatgg caccgttctc ctatctaatg ctcggtcgta
     2761 tcgctgaaga gcatttgaac ccgctcggcg cggtcgagat gggcgtcgag caggatgtgg
     2821 atcgcctgac gaagtttctg gatgcgtgga tcggcgttca gtacgtcggc gccgccggcg
     2881 tttttttcca cgcgatccag gtccgccgcc agcgacaggc atcgccaacg aatctccagg
     2941 aacgtctcgt ccagcgtctg ctttgcgccg cgcatggcgc gagtatagcg tggcgcgggc
     3001 gctgtgtccc acgctgttgg ggaggttgag ggtgatgggt tgctccgatg acgaaacagg
     3061 ttcaccgttt attgaccgct aggttgataa agcgcgtccg cggattttcc cctctcccgg
     3121 tacgccggga gagggctggg tgagggtgat ttcggatgtc aaacggctct agataaatag
     3181 aaatcaccct caccctaacc ctctcccgga ttaccgggag aggggaccag atggcagcgc
     3241 gctttaaata gccggtgctc agtagccgcc gcggccgcgc agcgtagtcg gagaaaaacg
     3301 ccgccaggcc gcggcggcta aaccaggaaa cctggggcgc tcgaatctgc ggcatcaaat
     3361 cccggcgctg agggtcccaa ggacctgatc ggtcgctgat gatcgtcagt cgctcaggcc
     3421 gtcgttcgac ggattattgc gattccaccc ggcgttcacg gattttcttt gatccactac
     3481 ccgcggcgcc cgtaatatgc tcagcgttgt tgatcgccgc tcgacggcgg tcgcgactcg
     3541 gcggccggca gatgcggatc tggctggccg cgatgttccc gggcaggcgt gtccgggaga
     3601 aagagcccgg cggcgggcag tagaaggcgt ttgctttggt cggtgtcccg gaagcggcgt
     3661 gagccgtctt ccaaaccgcg accgacagtc acctttgaag ttcccccgcg acggttgggc
     3721 aaggccgcga gccccgcttc gctgaagcgt gtatggctcg actgaaatgg aggtgatccg
     3781 tgaccaattg tgactgtacg tgtgggctgc tgtgagcagc ttccgcaagg tgcgattcct
     3841 gcggtacacg cagctgcttt tagcagctct gccggttcga ttgaacttca actgaaccgg
     3901 acagcagaga acacactgca aggcccggcg agcatggctc gccgggtctt ttttgcgcga
     3961 cttcgcctgt tccgggtgat caaatgcgaa cgcaaaggca cgaaggcgcg aaggaaaacg
     4021 cgaagacaag gcggaatcgg ttttcgttct tcctaccttc gcgtctgtct tcgttttcgc
     4081 gccttcgcgt tcgcgtttct ttccgcagtt gggagaagaa actttttatc cctgcaacgt
     4141 ggtcgaacgc gaactgtgtt cagacgcgca cgcctgcagc tttcaatgct gcctcgattt
     4201 tattgaaggt cgattcgtcg gggttgtggc ggcctttctc gaggcgattc aatgtctcaa
     4261 ctctgatttt tgcccggcgc gccagttctg cctgcgtgag gccagcgcga cgcctcgcca
     4321 gcacgagctt tcgcgccagc agggcgcggc catacgccac ggcggggacg tttccttcct
     4381 catcgggcgc gggcaagggc ggcaggcctg cctcaagcga gtcgccaccg ttggcgcgag
     4441 cgaggcgttc gtactcgcgg cgctcgatga ccacgaattc cttccctgcc agatgcagcg
     4501 tttgtacgtc agccatttta gttgtcataa acatctcggc ggttatcgat ctgccagaca
     4561 attactgttt cccccttcac gcggaacagc acgcgccaag caccggagcg aatccgaaag
     4621 tgaccgacga gctcttttct catcggcttt gcgccgctca cttccggcca cttctcaaga
     4681 cggaagaaaa catcgtttat tcggcggagc atgcccccgg gcagccggtt catctgttcc
     4741 tgtgccgccg gggtcagcac cacgttcgcc atggtggaag actagagcga ttgacaaata
     4801 atgtcaatta gaacaccgct ttcgatgccg ctgccgaaag gtctttggct gagattcagt
     4861 tgctcgacgc catcgtcggc gccggcttcg ccagccgcgc ttgcgccgtc tgcgccgcgg
     4921 gcggcggttc gagatacatc ggcccccacg tgttcgggcg gaactgcgtc atgacttcct
     4981 tcggcgccga ccagaagtaa tcgatcgcgt gttggaagtt gcggatgtgc aggttgaacg
     5041 ccatcgcggg ctgggtcttg gggtcccagc cgtgcagggc tttggcgggg atcagcatct
     5101 cgacgacgta gcgatccggc aggatgcgcg ttgcgctgcg gatgtcggga tgggggattt
     5161 ggttgtcatt aatagcatcg ccttcgcggt gccattgtcc aaccacaccg ccgacgccgt
     5221 tggtggcggt caggtcgttg ggcacgaaga agaactggtg gcagttcacg tcataggtgt
     5281 tctggtcgct ggcgacgggc ttggtgctga tccagaattc gacgttgtcc ttggtccacc
     5341 accagccctt ggcgggggcg ccttcgatgt cgttgtcgaa cacttccagg ccgacgtaca
     5401 ggccgtcgtt gctccaggcg aggtagacgt tcggcagcgg cagctggctg cgatccaagc
     5461 ctatcgtctg cgaatgccgg acggccggca gcttcgcttc gggcggccaa tcgcttagct
     5521 cgccgttaat gttcagcggc ttggtcagga agggcacgtg ggccaccggc ttggtcgcga
     5581 aactctcgcg ccaaacatcc tgctgatcgt ccttggcgct gttggcgtgc agaccgttca
     5641 gcagcggccc cgtcgcgcgc accgcgtcgg ccaacgcggg atacgggcgg tcgtcgatat
     5701 cgacgatgcc gaagttcacg tcttccccat cgctgctgcg gccgctcggc ggttcgtcca
     5761 tccactgaaa ccaatccgcg ccgatcacgt aaggcagccg cgccaggtgc gtcgtcatca
     5821 ggcgataacc atctgcccgc gcctgttggt ccggcacctg tgcgttgaag ccgacgagat
     5881 tgcgatcacc gctgcggcca tcgagtgagt ggaatgaata ttcgctgatg atcaccggct
     5941 gattgccggt ctcgtacatc atgcggaact gatccaggtc cagccgggcg tcgccgacgt
     6001 agtagttcag cgactgcgca tcggtgtatc ccgcggcggc gcgaacgact tccggcgggg
     6061 catagccgcg gaagcgcacg ccgagcacca ggtgattcgg gtcatacttg tgcacgaggg
     6121 tcgtggtgat gcggaagtag tcggccgcca ggtgcgaaag ccaggcggtg aagaggcgcg
     6181 tgtaggcctt aggctccagt tccttgggca ggtggtccca attctcaacg tctcgccaat
     6241 cgctgatgcg cgcccgccaa tccttgttga actgctcagg cgtggtccag acggtttgga
     6301 tgacgcgaag gacctcggcg cggttgggat cgtttttggg cagatgatcg aagtaggtgg
     6361 atgggccgga cgaaccatcg ccccaatcca gttcgttgtc ggtgtagtaa ccgacgaggt
     6421 tcttgttgtc gcgcagcaca tcgcactggg ccttaatggc cgcctcggcg gcgggggccc
     6481 agtcggggga gtagagccgc ttggccccgc tggcgatcca gtcccaaacg ttcaggtcgc
     6541 gcgtgatcgg gatatcgtac ttgtggaaga ccggatggca ccacgcgccc agccccttga
     6601 agccggtgtc gtgaacgcgg gcaattgtcg cctgggccca gcggtcgaga tcgccggtcg
     6661 ccgttgcgcc gccgttccaa tccgtagaga cgaatgaagg gccgttcaca tcgcggcctt
     6721 tttgaaatgg ctggaccgtc gtgacggtgt tgaggaagtc ggccttgtcg gcgggggaca
     6781 agaaccacca cgcgccgctc tggtcctgca cgatccgcca aaacccttca tgaccacgga
     6841 cgaccttata cagcggctgc gtgcttggcg ccgtggtggg ttgagtcgcc ggcgcgatcg
     6901 caaccgcatc ttccgcccgg ctgaacaatg gcccgtgcgc caccgccgcc agcagcgcta
     6961 cagaactgaa gatgacgagc agtcgcttca ccggttttgc cgcctgttag atgctatacg
     7021 caagcgcgca gcatcatggg cgcggctatg gaat
//
LOCUS       DATIMH010001083         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4588403, whole genome shotgun sequence.
ACCESSION   DATIMH010001083 DATIMH010000000
VERSION     DATIMH010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4588403"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VLI90_18730"
     CDS             complement(<1..629)
                     /locus_tag="VLI90_18730"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV16306.1"
                     /translation="MARSTPAGMSVLHRLFGGWTVAVLTFLYLPIVLLVVYSFNASRL
                     NITWQGFTLQWYRALWHNDPLIDSLNNSLIIAAITTVISVILGTAAAWVLDRYRFPLG
                     RAVVGLIYVPIIIPEVIMGISLLILFAIVSQHVPLGLGFTTVILSHVTFCFPFVMVAV
                     QARLAGLDPSLEEAAMDLGATPMRAFFRVIVPYLLPAIISGALMAFTLS"
     gene            981..1847
                     /locus_tag="VLI90_18735"
     CDS             981..1847
                     /locus_tag="VLI90_18735"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HSV16307.1"
                     /translation="MRIVRTISCALLAILLLPAALLAGRGDELVHASLVADVSAIQPG
                     ESFRLGVAVKIDPSWHIYWKNPGDSGLPTRVKLDLPAGFTAGEVQFPVPRKLTLPGDI
                     VNYGYEDQTMLIVPVSVAKDVAIGSTANLKAKISWLVCQEMCLPGKADVEITLPVAAQ
                     AQPVNREAFDQWSKQMPERDAREYVDEPWLVRSGNRQFEFKLSWKRAVTDVDWWPGNN
                     DPLTVRGVKVQTRDGATLLSFGVQAVDVPHPPDLVRSLLVFTLDGQRHAAKLNIPLPK
                     GWAAAEASSVSK"
     gene            1896..2498
                     /locus_tag="VLI90_18740"
     CDS             1896..2498
                     /locus_tag="VLI90_18740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSV16308.1"
                     /translation="MRFARTLICSMLVVAAAALLARAADGPKAVVGQPAPTFTLQDQD
                     GKPVSLSDFAGKIVVLEWVNPNCPFVQRHYAAKTFQTMDDEYRGKGVVHLAINTTHDA
                     TNAFNKDWITKNNLTYPILNDSDGVVGKAYGAKTTPELYIIGRDGKLLYMGGIDNDRD
                     GDKGSSKVNYVAKALDEILAGKTVSTPETASYGCSVKYGS"
     gene            complement(2578..>3083)
                     /locus_tag="VLI90_18745"
     CDS             complement(2578..>3083)
                     /locus_tag="VLI90_18745"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HSV16309.1"
                     /translation="IGDAPRDIEAGKAAGCRTILFQDPTLPASPAASATPLVLPDHYV
                     QSLKDAMDIIERADVQAHAPPVVMSSPVAPLPPAIDLQRLESLSEQILQELRKPAPAP
                     AVQTSPPPPAAEPHFSFSKMMAGVVQVLAVAVFALAYRGGNVEQMLVGLMLQLLTIAL
                     LIMGRQK"
BASE COUNT          621 a          876 c         1005 g          581 t
ORIGIN      
        1 atcgacagcg tgaacgccat cagcgcgccg gagatgatcg ccggcaacag gtacggcacg
       61 atcacgcgga agaatgcgcg catcggcgtc gcacccaagt ccatggcggc ttcctccagc
      121 gatggatcga gcccggccaa tcgcgcctgc accgcgacca tcacgaatgg aaagcagaac
      181 gtcacatgtg agaggatcac cgtggtgaag ccaagcccca gcggcacgtg ctggctcacg
      241 atcgcgaaca ggatcagcag gctgatgccc attatcactt ccgggatgat gatcggcacg
      301 tagatcaatc ccaccacggc ccgtccgagc gggaagcggt atcgatccag cacccacgcg
      361 gcagcggtgc cgagaatgac cgagatgacg gtcgtgatgg ccgcgatgat gaggctgttg
      421 ttgagcgagt cgatcagcgg gtcgttgtgc cagagcgcgc gataccactg cagcgtgaac
      481 ccctgccagg tgatgttcaa gcgcgatgcg ttgaacgagt agacaacaag cagcacgatc
      541 ggcaggtaaa ggaaggtgag gacggcgacg gtccacccgc cgaacaggcg gtgcagcacc
      601 gacatccccg cgggggtgga acgtgccatg gcggtaagag tgtcgcgagc gcgagcggtc
      661 atgcaagtct ctgattgctg cgttctggat gcgcgtgatg aaaggcaacc gccgagggcg
      721 ccgagagcgc cgagatagcc gcagagaaga cggcaaatgc agaagagttt taaccgcaga
      781 tttcgcagat tacgcagatt agaagaggct cattctgaat ctgcgaaatc tgcgtaatct
      841 gcggttccaa ctcttcctcc tctcggcgtc cgtctcggcg tgctcggcgc cctcggcggt
      901 tgcattgtat gaacggtggt atttcatgcg agaattttgc cgctgcccgc gcgatttaga
      961 tgaatacaat tgcttccccg atgcggatcg ttcgaaccat cagctgcgcg ctactggcca
     1021 tcctgctcct gcccgcggcg ttgcttgccg ggcgggggga tgagctggtg catgcatccc
     1081 tggtcgccga tgtctcggcg attcagccgg gcgaatcttt tcgcctcggc gttgccgtga
     1141 agatcgatcc gtcatggcac atctattgga agaacccggg cgattcgggt ctgccgacgc
     1201 gcgtgaagct ggacctgccc gcggggttca ccgcgggtga ggtgcagttt ccggttccca
     1261 ggaaactcac gttgcccggc gatatcgtca actacggcta tgaagatcaa acgatgctga
     1321 tcgtgccggt cagtgtcgcg aaagatgtgg ccatcggatc gacggcgaac ctgaaggcga
     1381 agatcagctg gctcgtttgc caggagatgt gcctgccggg caaggcggat gttgagatca
     1441 cgctgccggt ggcggcgcag gcgcaaccag tcaatcgtga agcgttcgat cagtggtcga
     1501 aacagatgcc ggagcgcgat gcgcgcgaat atgtcgatga gccatggttg gtccgcagcg
     1561 gcaatcggca attcgagttc aagctgtcct ggaagcgagc cgtcaccgac gtcgattggt
     1621 ggcccggcaa caatgatccc ctcacggttc gtggcgtgaa ggtgcagacg cgcgacggcg
     1681 cgacgctgct gtcattcggt gtgcaggcgg tcgatgtgcc gcatccgccg gatctggttc
     1741 gttcgctgct ggtcttcacg ctcgacggcc agcggcacgc ggctaagttg aacatcccgc
     1801 tgcccaaggg ttgggcggcg gcggaagcat catcggtttc caaatgattt catttacgcg
     1861 cggccgccgt tgccgctgcg ctgaaggagc ttcgcatgcg attcgcccgt acgttgattt
     1921 gttcgatgct cgttgtcgcc gccgcggcgt tgttggctcg cgccgccgac ggtcccaagg
     1981 ccgtggtcgg tcaacccgcg ccgacgttta cactgcagga tcaggacggc aagcccgtga
     2041 gtttgtccga ctttgctgga aagatcgtcg tgctggaatg ggtgaatccg aattgcccgt
     2101 ttgtccagcg gcattacgcc gccaagacgt tccaaacgat ggacgatgaa taccgcggca
     2161 agggcgttgt gcatctggcg atcaacacga cgcacgatgc gaccaacgcg ttcaacaaag
     2221 attggatcac caagaacaac ctgacgtatc cgatcctgaa cgactccgac ggcgtggtgg
     2281 gcaaggcgta cggcgcgaag acgacgccgg agttgtacat catcgggcgc gacggaaagc
     2341 tgctgtacat gggcggcatc gacaatgacc gcgatggcga caagggatca tcaaaggtga
     2401 actacgtggc gaaggcgctg gatgaaatcc tggctggcaa gacagtgtca acgcccgaga
     2461 cggcgtcgta tgggtgcagc gtgaagtatg ggagctgatc ggttccgcgt gtaaagccgg
     2521 cgatggtatt gccgaggtga ataccggcga tgacatcggc ggctttacgc tgttgtttta
     2581 cttctgtcgt cccatgatca ggagcgcgat cgtgagcagc tgaagcatga ggccgactag
     2641 catttgctcc acattgccgc cgcgatacgc caaggcgaag accgcgacgg ctagcacctg
     2701 tacgactccg gccatcattt tcgagaacga gaagtgcggc tccgccgccg gaggcggtgg
     2761 cgaggtttgt acagctggcg cgggcgctgg tttccgcaat tcctgaagga tctgctccga
     2821 cagtgattca agccgctgaa gatcgatcgc cggcggcagg ggcgctaccg gtgaactcat
     2881 caccaccggc ggcgcgtggg cttgcacatc cgcgcgctcg atgatgtcca tcgcgtcctt
     2941 caagctctgc acataatgat ccggcagcac cagcggtgtc gccgacgccg cgggactggc
     3001 gggcaaagtt ggatcctgga aaagaatcgt ccggcacccg gcggctttgc cggcttcgat
     3061 gtcgcgcggg gcgtcgccga tca
//
LOCUS       DATIMH010001084         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5461009, whole genome shotgun sequence.
ACCESSION   DATIMH010001084 DATIMH010000000
VERSION     DATIMH010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5461009"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..961)
                     /locus_tag="VLI90_18750"
     CDS             complement(35..961)
                     /locus_tag="VLI90_18750"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HSV16310.1"
                     /translation="MSVLSTDTLGRDGRSVSAARSRAYCEQLTRSQAKNFYYGLKLLP
                     EPKRSAMFTLYAYMRLIDDIADDEDGRSVRQRIDELEQWREHTHAILDGRSVEARTEV
                     VDDGHLLWPAFAEMVNDYRMPRHIFDDVIAGQRQDLEPEPFDTFAQLREYCYRVAGVV
                     GLASIYIWGFECGAETEALAIDRGVAFQLTNILRDLHEDAARGRTYLPREELAVLNVS
                     EADLKDGRGGREFHELMRFQIDRAEAYYQKSRPLEGRIQRDSRPTLVAMTQIYHRLLK
                     KVATEPERVLRERVSLSLISKLMIGWRAARAK"
     gene            complement(958..1965)
                     /gene="hpnC"
                     /locus_tag="VLI90_18755"
     CDS             complement(958..1965)
                     /gene="hpnC"
                     /locus_tag="VLI90_18755"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HSV16311.1"
                     /translation="MSTTSHPAGRPATVDPLHADSARPESATPTQSPAPATVEMAFAP
                     FTTARAAQSYTWQLAHSHYENFSVISVLLPRHLRQDFCNVYAFCRIADDLSDEVVDRD
                     ESLRLLERFRAETLAMYAGTPRTVIFGALAETVKRYDIPVQPFLDLVDAFEQDQRVDR
                     YHTFAQLCDYCRRSADPVGRLVLYMCGYRDEQRQRLSDRTCTALQLANFWQDVRRDIV
                     DRDRIYLPRETMQQFGVTEQQIRDGRCDDNYRKCIQFEVDRTEAMFAEGDALLPLLNE
                     AVRPQVSLFGQGGRAILDAIRRQDYDTLTNRPSLSRWQKGRLVFGAVAARIRQVVEVS
                     A"
     gene            complement(2185..2550)
                     /locus_tag="VLI90_18760"
     CDS             complement(2185..2550)
                     /locus_tag="VLI90_18760"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HSV16312.1"
                     /translation="MLNNPRTPATRRVGYLLPAAALLLALLSIGADDQGSTQNVTIQD
                     MSFSPTSVHAKVGDTVIWTNADDQDHTVIAADGSFNSGDIGSGRSFQHKFTRAGTFGY
                     YCKYHPRMKGNVVVAAKDR"
BASE COUNT          440 a          823 c          869 g          490 t
ORIGIN      
        1 ccccgcactg aagtgcgagg gcctcattgg tggttcactt cgcccgcgcg gcgcgccagc
       61 cgatcatcaa tttgctgatc agcgaaagtg atacccgctc gcgcaaaaca cgttcgggtt
      121 cggtggcgac cttcttcagc aggcggtggt agatctgcgt catcgcgacc agcgtcggcc
      181 ggctgtcacg ctggatgcgg ccttcgagcg ggcgcgactt ctgataatag gcttcggccc
      241 gatcgatctg gaagcgcatc agctcgtgga actcgcgtcc gccgcggccg tctttcaggt
      301 ctgcttccga cacgttcaac accgccagct cttcgcgcgg cagatacgtc cgtccgcggg
      361 cggcgtcttc gtgcaggtcg cgcaggatgt tcgtcaactg aaacgcgacc ccgcgatcga
      421 ttgccagcgc ttcggtttcg gccccgcatt cgaagcccca gatgtagatg ctggccagac
      481 cgacgacgcc ggcgacgcga tagcagtatt cgcgcagctg cgcgaacgtg tcgaacggct
      541 cgggttccag gtcctgccgc tggccggcga tcacatcgtc gaagatgtgc cgcggcatgc
      601 ggtagtcgtt caccatctcg gcaaaggcgg gccagagcag gtggccgtca tcgacgacct
      661 ccgtgcgcgc ttcgacgctg cggccatcga ggatcgcgtg cgtgtgctcg cgccattgtt
      721 ccagttcgtc gatgcgctgg cggaccgacc ggccatcttc atcgtcggcg atgtcgtcga
      781 tcagccgcat gtacgcgtag agcgtgaaca tggctgagcg cttcggctcg ggcagcagct
      841 tgaggccgta ataaaagttc ttggcttggc tgcgggtcag ctgttcgcaa tatgcgcgcg
      901 agcgcgctgc gctgacggag cgtccatcgc ggccgagcgt gtcggtgctc agcacgctca
      961 cgccgacacc tccaccactt ggcggatgcg tgccgcgaca gccccgaaga ccagccggcc
     1021 cttttgccag cgcgacagcg acgggcgatt ggtcagcgtg tcgtagtcct gccggcgaat
     1081 ggcgtcgaga atcgcgcgcc cgccttgtcc gaacagcgac acctgcgggc gcaccgcctc
     1141 gttcagcagc ggcagcagcg catcgccttc ggcgaacatc gcctcggtgc gatcgacctc
     1201 aaattgaatg catttacgat aattatcgtc acagcggccg tcgcgaatct gctgctcggt
     1261 cacgccaaac tgctgcatcg tttcgcgcgg cagataaatg cgatcgcgat cgacgatgtc
     1321 ccggcgaacg tcctgccaga agttggccag ctgaagcgcg gtgcaggtgc gatcggacag
     1381 gcgttgccgc tgctcgtcgc gatagccgca catatacagg acgagccggc cgaccggatc
     1441 ggcgctgcgg cggcaatagt cgcagagctg cgcgaacgtg tggtaacggt ccacccgctg
     1501 gtcctgctcg aacgcgtcta caagatccag aaacggctgg accggaatgt cgtagcgttt
     1561 caccgtctcg gctaatgcgc cgaagatgac cgtccgcggc gtgccggcgt acatcgccag
     1621 cgtctcggcg cggaaacgct cgagcaaccg cagcgactca tcgcgatcga ccacttcgtc
     1681 gctcaagtcg tcggcgatgc ggcagaaggc gtagacgttg cagaaatcct gccgcaggtg
     1741 ccgcgggagc aggacggaga tgaccgaaaa gttttcgtaa tgcgagtggg ccagctgcca
     1801 ggtgtaactt tgggccgcgc gggccgtggt gaagggggcg aaggccatct cgacggtggc
     1861 cggggcgggc gattgcgtcg gggtggcgga ctcggggcgc gcgctgtccg cgtgcagcgg
     1921 gtcaaccgtc gcgggcctgc ccgcggggtg gctcgttgtg gacattgatc ccattcacaa
     1981 ccgcctgccg gcgaagggtt tcggcgtcat ccaagcgacc cctgccgctc gaacgtcttg
     2041 gggcagcaac ggctctcagc ggtggatact acagcgacgg tgcgggcttc gcaacgcgag
     2101 agataaggtg gcgacatggg gggcacgtga cgcgccttga atgaattccg gtcccttccc
     2161 cggcctcagg gctggcccgc tgcgttatcg gtccttcgcc gcgacgacca cgttcccctt
     2221 catccgcggg tggtatttgc agtagtagcc gaacgtcccg gcgcgggtga acttgtgttg
     2281 gaacgaccgc ccgctgccga tgtcgccgga gttgaacgag ccgtcggcgg cgatcacggt
     2341 gtggtcctgg tcgtcggcgt tggtccagat gacggtgtcg ccgaccttcg cgtgcaccga
     2401 tgtgggcgag aacgacatgt cctggatcgt cacgttctgc gtcgatccct ggtcgtcggc
     2461 cccaatcgac aacagcgcca gcagcagcgc cgcggcgggc aatagatatc cgacgcggcg
     2521 ggtggccggg gtgcgtgggt tgttcagcat gatctcgctc ctctaatata acgccgcgtc
     2581 tgctgcttat tgtgttagac actcttatcg accactggac ag
//
LOCUS       DATIMH010001085         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1416028, whole genome shotgun sequence.
ACCESSION   DATIMH010001085 DATIMH010000000
VERSION     DATIMH010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416028"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..867)
                     /locus_tag="VLI90_18765"
     CDS             complement(58..867)
                     /locus_tag="VLI90_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16313.1"
                     /translation="MSSDDSKFDSVDQVIKSAAKRAWCAECAPASLRQCVQDLMRCSE
                     MPSMRYRDRRWMLWPLSLAAVIALAVGIKFRVSATTHSPASPALASLSHLPALPASLQ
                     ADLVKSHEHCCHAADHHHLNASKDDDVAIAQAMRQRLSRAVLMARPADTGWYFRGAAI
                     CPVGDAPAGHLVFAKNDDVVSVFSLPMAAIPNATDGEQFQTTIDGHPIAAFTKDGGLF
                     CLVSSGPLGSLTEKDLAQMRDRMEDRVTTASGSLTPAHDAVAVDLMYHVWH"
     gene            complement(967..1518)
                     /locus_tag="VLI90_18770"
     CDS             complement(967..1518)
                     /locus_tag="VLI90_18770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV16314.1"
                     /translation="MSLTSDEFERLALAELDPLYRMARRLTRDSNRADDLVQETYARA
                     LRSRDNFSLQQFGIKPWLLRIMYNLHLNRADRARREPQAVEDDALEAGANAANSDGAD
                     LPIDPSSFQAMDEQLVHALDDLPEDYRAIVLLWAVEELSYKEIATALDIPIGTVMSRL
                     HRARHRLAEHLREYAVTEGIIRE"
     gene            complement(1629..>2513)
                     /gene="ilvC"
                     /locus_tag="VLI90_18775"
     CDS             complement(1629..>2513)
                     /gene="ilvC"
                     /locus_tag="VLI90_18775"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HSV16315.1"
                     /translation="VIVANRKDSANGRLAIEHGFDPVSVEDATKAADLIIVTLPDEVQ
                     PDVWKKSVAPNLKAGTAIGTTHGFNIHFKTIVPPKENDVLLVAPKGPGHLVRSEFEKG
                     GGVPCLLAIHQNATGKARDIGLAWARGIGGARSGIIETTFKDECETDLFGEQAVLCGG
                     LSALIKAGFETLTEAGYPPEMAYFECVHEVKLIVDLIYQGGLDYMRYSISNTAEYGDL
                     TRGPRVVDSRAKAEMKKILSEIQSGQFAKEWRAEYEAGMKNFKRLYEADNNHPVEVTG
                     RRLRKMMPWLKAKEPPKS"
BASE COUNT          465 a          770 c          757 g          521 t
ORIGIN      
        1 accggttaaa tcaccgattc aattgaaatg cgcgatttag atttgcggag gcaaacgcta
       61 gtgccacaca tgatacatga gatcaactgc gacggcgtcg tgggcggggg tgagcgagcc
      121 ggaggcggtg gtcacgcggt cttccatgcg gtcgcgcatt tgggcgaggt ctttttctgt
      181 caatgaacct aaagggccgg agctgacgag gcagaacagg ccgccgtctt tggtgaacgc
      241 ggcgatgggg tggccgtcga ttgtcgtctg gaactgttcg ccgtcggtgg cgttggggat
      301 ggcggccatg ggaagtgaga agaccgagac gacgtcatcg tttttcgcga aaaccaggtg
      361 accggcgggg gcgtcgccaa cggggcagat ggcggcgccg cggaagtacc aaccggtgtc
      421 ggcggggcgg gccatcagca ccgcgcgcga cagccgctgt cgcatcgcct gcgcgatggc
      481 aacgtcgtcg tccttcgatg catttaaatg gtgatgatcg gcggcgtgac agcagtgctc
      541 gtgagacttc actaaatcgg cttgaagcga ggcgggcagg gcgggtaggt gggaaaggct
      601 ggcaagggca ggactggcgg gcgagtgggt cgtggcggat accctaaact taatgcctac
      661 agccagggca atcacggcgg cgagcgacag cggccagagc atccagcggc gatcgcgata
      721 gcgcatcgac ggcatttccg agcagcgcat taaatcctgc acgcactgcc gcagcgaggc
      781 gggcgcgcac tcggcacacc aggctcgctt ggccgcggat ttgatcacct gatctacgct
      841 gtcaaacttc gaatcgtcgc tggacatatc ggtcactgct cgttgcttct ctccgcacgc
      901 ttgcccccgg ccacctcgcg tgcttacctc acactaacaa taggattaac cagcgggaaa
      961 catccgctat tcccggatga ttccctctgt tacggcatat tcgcgcaaat gttcggccag
     1021 gcggtgccgg gcccggtgca accggctcat caccgtgccg atcgggatgt ccaatgccgt
     1081 cgcaatttcc ttgtacgaca gctcttccac cgcccacagc aggacgatgg cccgataatc
     1141 ttccggcagg tcatccagcg cgtggaccag ctgctcgtcc atcgcttgaa agctggacgg
     1201 atcgattggc aaatcggcgc cgtcagagtt agcggcgttc gcgccggctt ccagtgcgtc
     1261 atcttccacc gcctgcggtt cccggcgggc gcgatcggct cggttcaggt gcaggttgta
     1321 catgatccgc agcaaccacg gcttgatgcc gaactgctgc agcgaaaaat tgtcccggct
     1381 gcgcagcgcg cgggcgtacg tttcctgcac aagatcgtca gcgcggttgg aatcgcgcgt
     1441 caaccggcgg gccatccggt agagcgggtc cagctctgcc agcgccaacc gctcgaattc
     1501 gtcgctcgtg agactcatgt gatcgtcacc taaaacagag acgccgctcg cggcttcgca
     1561 aacccaaggc actcggattt gcgaagccac cagcggcggc tgtacttcaa aattcgcgaa
     1621 tcgcggagct aggatttcgg tggctctttt gccttgagcc atggcatcat cttgcgcagt
     1681 cgtcgtcccg ttacttctac cggatgattg ttgtccgctt cgtatagccg cttgaagttc
     1741 ttcattcccg cttcgtattc ggctcgccat tccttcgcaa actgaccgga ctgaatttcg
     1801 ctgagaatct ttttcatctc agccttggca cgcgagtcaa caacgcgcgg tccgcgggtc
     1861 agatcgccgt attcggcggt gttgctgatg ctgtaccgca tgtaatccag gccgccctgg
     1921 taaatcaggt cgacgatcag cttcacctcg tgcacgcatt caaagtacgc catctccggc
     1981 ggataaccgg cttcggtcag cgtttcgaag ccggccttga tcaacgcgct cagtccacca
     2041 cataagacgg cttgctcgcc aaataaatcc gtctcgcatt catctttgaa cgtcgtctcg
     2101 atgatgccgc tgcgcgcccc gccaatgccg cgcgcccacg ccaggccgat gtcgcgcgcc
     2161 ttgccggttg cgttttgatg gatcgccagc aagcacggca caccgccgcc tttttcgaat
     2221 tcgctccgca ccaaatggcc cggacccttg ggggccacca gcagcacgtc gttctctttc
     2281 ggcggcacga tcgtcttgaa atggatgttg aacccgtgcg tcgttccaat ggccgtgccg
     2341 gccttcaggt tcggcgccac gctcttcttc cacacatccg gctgcacttc gtcgggcagc
     2401 gtgacgatga tcaggtccgc cgccttggtc gcgtcttcaa ccgagacggg gtcgaaccca
     2461 tgctcgatcg ccagccggcc gttggcggaa tctttgcggt tagcgacgat cac
//
LOCUS       DATIMH010001086         4304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3433338, whole genome shotgun sequence.
ACCESSION   DATIMH010001086 DATIMH010000000
VERSION     DATIMH010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3433338"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2315)
                     /locus_tag="VLI90_18780"
     CDS             complement(<1..2315)
                     /locus_tag="VLI90_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16316.1"
                     /translation="MPLRRRRPLGHRSAATESLVRLFHRFGGGRAGAGSGGEGQRPAG
                     GDAAPARLTRAVHYGLEHLEQRCMLTTIGFDIPSLTQIPDGVIEYSQGQPGNIVRVAW
                     HDIVAELIGVSVPKTGSTTAPTPPDVPTPGEDLILAAPQPPDWAPNLFTIYILASKPD
                     SYLSIAVVPSLTATNRPMEPYGSGVSMRVTNAGPGAPIIAVSAGPGGVLIGQLHTGPT
                     ANGGDGEQPIWTAPATPPLGVFPGTFGAPLTAGVIMAPINPIDGTPNDIGELLIGGTV
                     LGAVNVPRAGTATAAGASQGGNIGLFYAGAVLTGTATGVLQSENPDSLQFGLGTSASD
                     ITPSFVVGGDIRDFVVGGSIGGDPGTQPNLPQYLTATRIVVGGTVGQFNTVGGSNYGL
                     FEIQHNPAVVGLQSSLYTDFEFEYKTLPGDAPGNAFQGGLELGFDSTTGIFVPLPPRL
                     AGLITNDSPATAQLLGSVKESDPRTGAAALDVNGNYTYESFVSGLLQDFAPNAPADNQ
                     DWYAVPLMAGQVITVTATTSLITTGVQGVSAGIPELQLEVMDPDGRIIATDENQQRPL
                     QNLDKPVSFRADRPGLYKFQLTNLPGVIFEGEIPYTLQVQGVGDMAVAAFSSSGDIFD
                     AEYDKSIQIDNGDLGALLAGAHLLSDTLGPTQPVDPVTLQPIPGYVPPTPTTWLVDHG
                     NLRVMQAPQIGFLGTGGAFGDIPYLDVPNASVGLVDATGPLLALQTRFDLLNTTNPDV
                     PVSPVGGGFQIISQDGGAANGGTTTNSLAYL"
     gene            complement(2724..3776)
                     /locus_tag="VLI90_18785"
     CDS             complement(2724..3776)
                     /locus_tag="VLI90_18785"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HSV16317.1"
                     /translation="MSLQSLIASGTKVWLDSIDPPLIKRNRALGATGATSNPIIIADI
                     IKSGHFDEQIAQLARKGGTDSEIAWDMDDRLVKEAQDVFLPVWEQTRGNDGYVSFELD
                     PLLEDKQNPTPTDVATRKYIELAKRWSAGHRNRMIKIPATAAGLAAMEEVAANGTTIN
                     VTLIFSERQYKIARDAIWRGAQRRKNGLDSFKSVYSIFVSRVDVYTEKNVPDLSPAAQ
                     GQVGIVNAKRIWQENQAFWKDKGLKLKQEMIFASTGTKKPTDPPDKYVEALAGSDIQT
                     NPPATNDAVEKLNKPYTRKVDQMPPQAVLDEIDQKVDQRKMEDKLMEEGTLKFADPQK
                     ALLKQIAEKRASLARA"
BASE COUNT          727 a         1367 c         1349 g          861 t
ORIGIN      
        1 tgcaggtacg caaggctgtt ggtggtcgtg ccgccgttgg ctgccccgcc gtcctggctg
       61 atgatctgga acccaccgcc caccgggctg accggcacgt ccgggttcgt cgtgttgagc
      121 aggtcgaagc gcgtctgcag cgcgagcaat ggtccggttg catcgacgag tcccaccgat
      181 gcattgggca catccagata cgggatgtcc ccgaacgccc cccccgtccc cagaaatccg
      241 atctgcggcg cctgcatcac gcgcaggttg ccgtgatcca ccagccaggt cgtcggcgtg
      301 ggtggaacgt agccggggat gggctgcagc gtcaccggat ccaccggctg ggtcggaccg
      361 agcgtgtcgg acagcaaatg agcgccggcc aacagcgcgc ccaggtcacc gttgtcgatc
      421 tggatcgact tgtcgtactc ggcgtcgaag atgtcaccgg aggagctgaa cgccgcgacg
      481 gccatgtcac cgacgccttg gacctgcagt gtatatggaa tttcgccttc gaagatgacg
      541 ccgggcaagt tggtgagctg gaacttgtaa agcccgggtc gatcggcgcg gaagctgacc
      601 ggcttgtcca ggttctgcag cgggcgctgc tggttttcgt cggtcgcgat gatccggcca
      661 tccgggtcca tcacttccaa ctgcaactcg gggatgccgg cggaaacgcc ttgcacgccg
      721 gtggtgatca gcgatgtcgt cgcggtgacc gtgatgacct gcccggccat cagcgggacg
      781 gcataccagt cctggttgtc cgccggcgca ttgggcgcga agtcctgcag cagcccggag
      841 acgaaacttt cgtaggtgta gttgccgttg acatccagcg cagcagcgcc ggtgcgcggg
      901 tcgctttcct tgacggatcc aagcagctgc gcggttgccg gcgaatcatt ggtgatcaac
      961 cccgcaagcc gcggcggcaa gggaacgaaa attccggtgg tcgaatcgaa tccaagttcc
     1021 agtccaccct ggaacgcgtt gcccggcgcg tcacccggaa gcgtcttgta ttcgaattcg
     1081 aagtcggtgt agaggctgga ctgcaaaccc acgacggcgg ggttgtgctg aatctcgaac
     1141 agcccgtagt tgctgccacc gacggtgttg aactgcccga cggtcccacc gacgacaatc
     1201 cgggtggcgg tgaggtactg cggcaggttc ggttgtgtgc cggggtctcc accgatgctg
     1261 ccgccgacga cgaagtcgcg gatatccccg ccgacgacga agctcggcgt gatgtccgac
     1321 gccgatgtgc cgagtccgaa ctgcagcgaa tccggattct cactttgcaa gacaccggtg
     1381 gcggtgccgg tcaacacagc gccggcgtag aacaggccga tgtttccacc ctggcttgcg
     1441 cccgccgcgg tggcggtgcc ggcgcgcggc acgttgacgg cgcccagcac ggttccgccg
     1501 atcagcagct caccgatgtc gttgggcgtt ccatcgatcg gattgatcgg cgccatgatg
     1561 acgccggccg tcagcggcgc gccgaaggtg ccgggaaaca cgcccaaggg cggcgtcgcg
     1621 ggcgcggtcc agatgggctg ctcgccatca ccaccgttgg cggttgggcc cgtgtgcagc
     1681 tgaccgatca atacgccgcc cggacccgcg ctgaccgcga tgatcggtgc tccgggcccg
     1741 gcgttggtga cgcgcatgga cacgccgctc ccgtacggtt ccatcgggcg gttcgtcgcc
     1801 gtcagcgacg gcactaccgc gatcgacagg tacgaatccg gcttggatgc gaggatgtaa
     1861 atcgtgaaca gattcggcgc ccaatccggc ggctgcgggg cagccagaat caggtcttcg
     1921 cccggcgtcg ggacgtccgg cggcgtgggc gcggtggtac tgcccgtttt cggcacgctg
     1981 acgccgatca actctgcgac gatgtcatgc cacgccacgc gaacgatgtt gccgggttgg
     2041 ccctggctgt attcgattac cccgtcaggg atctgcgtca gcgaggggat gtcgaacccg
     2101 atcgtcgtca gcatgcaccg ctgttcgagg tgttccaagc cataatgaac cgcccgggtg
     2161 aggcgcgcgg gcgcggcgtc gccgccagcg gggcgctggc cttcgccgcc gctcccggca
     2221 ccggcgcgtc caccgccaaa gcgatggaag agccggacca acgactcggt tgcagcagaa
     2281 cggtggccaa gcgggcggcg gcggcgaagc ggcatgcagg ttcctcctca accgacagag
     2341 tgcgttcgat ccccctcgac tgcgatcgct tcttgaccgt gtcgatgttg tcgcgcgcgt
     2401 accatctgat cggttggatt actcaattgc gacgcgctcg cgtacggctt tagtcgcgtg
     2461 gccgcgtctt gtgctggcgt gttgtaactt ccggcgcaaa cacacgatcg ccatcacgtg
     2521 tgtgaaggaa gcaggcacca ttttacgtaa ggcgttctat caccgggccc aacgagtgtc
     2581 aacgcggggt gggatgcgcg acatggggcg ctgacgcggg caagcgatgc agaatgatga
     2641 atgcagaatc cagaatcaaa gatgggacag cccgattgcc cgttttcatt cggcattctg
     2701 cattcatcat cattctgccg ttattaagcc cgcgccaaac tcgcccgctt ctccgcgatc
     2761 tgcttcagca gcgccttctg cgggtcggcg aacttcaacg tcccctcctc catcagcttg
     2821 tcttccatct tccgctggtc caccttctgg tcgatctcgt ccagcacagc ctgcggcggc
     2881 atctgatcga ccttgcgcgt gtacggttta ttgagcttct ccaccgcgtc attcgtcgct
     2941 gggggattgg tctggatgtc gctcccggcg agcgcttcaa catatttatc gggcgggtcg
     3001 gtcggtttct tggttcccgt gctggcgaag atcatctcct gtttcagctt caaccccttg
     3061 tccttccaga acgcctggtt ctcttgccag attcgcttgg cgttgacgat gccaacctgc
     3121 ccctgcgccg ccggcgacag gtccggcacg ttcttctccg tgtacacatc cacgcgcgag
     3181 acgaagatgc tgtagacgct cttgaatgaa tcgagcccgt tcttgcgccg ctgagcgccg
     3241 cgccagattg catcgcgagc gatcttgtat tgccgttcgc tgaagatcag cgtgacgttg
     3301 atcgtcgtgc cgttggccgc cacctcttcc atcgccgcca gccccgccgc cgtcgccggg
     3361 atcttgatca tgcgattgcg atgaccggcc gaccagcgct tggccagctc gatgtacttc
     3421 cgcgtcgcga cgtcggtcgg cgtcgggttc tgcttgtctt ccagcagtgg gtcgagctcg
     3481 aagctgacgt aaccatcgtt gccgcgcgtc tgctcccaca cgggcagaaa aacgtcctgc
     3541 gcttccttca ccaagcggtc gtccatgtcc caggcgattt cgctatcagt tccacccttc
     3601 cgggccagct gcgcgatctg ctcgtcgaaa tgcccggact tgatgatgtc ggcgatgatg
     3661 atcggattcg acgtggcgcc cgtcgcgcca agcgcgcgat tgcgtttgat gagcggtggg
     3721 tcgatcgagt cgagccacac ctttgtgcct gaagcgatca gcgattgaag cgacatgtga
     3781 acctcacgag ttagatttgc gcgcgtcccc gcgcacattg gccgggatca tagccgacgc
     3841 ggcggcgcgt gaaacctgaa atccccgccg cttgcggctt agcgtcgtta ccggccggaa
     3901 aaccgcgcat tttacctgcc tgcccacctg acacatccct gtcacgtgaa tcgaaacgac
     3961 agcaacaatt cgtcgtggtc gtacgtcatc ggcaagcgga ctctaccgaa accgcgcgtc
     4021 cgcagtccaa cctcggcgca ggatggcatg tcggttgtat ctactccgat cagtctgctg
     4081 cgccacacgc acgcgcgtcg catcaggttt ttcgtggagt gtgcgatttt ccccctacac
     4141 gtgggcgagg ggttcgtcgc atgattatcg cgtgtcccac aaatcactct tacggcttgg
     4201 aggaaacatg atccggaaag ctttggtctt cacagcgctg ctcctggtcc ccgctatcgc
     4261 aaaggccgac ggcaacattg cccgcgatgc cgggccgttc gaag
//
LOCUS       DATIMH010001087         4487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3725010, whole genome shotgun sequence.
ACCESSION   DATIMH010001087 DATIMH010000000
VERSION     DATIMH010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3725010"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1179)
                     /locus_tag="VLI90_18790"
     CDS             complement(28..1179)
                     /locus_tag="VLI90_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSV16318.1"
                     /translation="MGTSEAQKGTAMTEKLITPQAMVGRRTLLRTAAVAGVVALAGET
                     AQAKKDRDNDRDDDREHGRLTRGDAAILRFLAAAEILETDLWQQYNELGGIQDDEVPG
                     GSGNPAYTEALAVLDEDMAQYIHDNTENEMTHELFLNAYLEAHGAEPADLERFRTLPS
                     SQATGAQQIGRLTNLMRLTLDTSWWSRYRDPSDNPDLDPDFIFPQAVPDLHNGQFPGI
                     PRTDADLAPAKHLQAIANTAGFHFPSVEVGGTSLYPSLAQRITGAEALRVVLSIGPTE
                     AMHFQTWQDKAGNAPPLTDPTNGLTFPDLSNAGELLQSNLIMPEPCPFLSTDLPICSI
                     VRPTETKGAAKGALAYLTAMGIFRGQSAGFFAYMRGLAADADAARRGGP"
     gene            1866..>4487
                     /locus_tag="VLI90_18795"
     CDS             1866..>4487
                     /locus_tag="VLI90_18795"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSV16319.1"
                     /translation="MRLHNVRQSDIASAGLVLRRRRAMLAEALEQRLLFASLRDFVGL
                     SAETSSRLLGFVSTAHGGSGQQQQQTVVAGDGLTDSVWLANHVDLPTIALLDKVDGPL
                     QQMVAQSLRDGKSFADMSIGNAPPSDQYEHAGDDPLVTIRSAEPTALAAKLRALGFNV
                     SGSVENGEAGVVDVYTPVSRITELAGVAGVKSLGAEQNLPTDAGSISSTWPGTANIPQ
                     LLLDRPGMTGAGIKYGIISDSFNNLGGMAAGIATGDLPAGVTILHDAAGGGTDEGRAM
                     AEVLYDAAPGSNILFYDSGGSKTGMVTAINALSAAGANVIVDDVNEVEGTFQDDVLSQ
                     AIDAVVLNKNIPYFTSSGNRNGQTWYGAFNEGIVVDSYNAFDGAAIQNLLVTIPANGT
                     ITVDLWWGQKLGGATTDLDTFLNDANTGTSLASATGVEIGGDAFTGFGYTNNTGATIT
                     GSISLRKAGGTSAIGLPLFLLTFPGKGTAPTGPFWGGPAAYGHHMSEFGFGVAAVQPA
                     SPNTIESFSSLGGVPILFNDDGTAVSGGSVIRNQPALAACDGINTTFFGQFFGTSCAA
                     PDAAAIAGLLLQAAGGGGTLTYSQMRDMLSNSAFDLGSAGYDLTYGAGRIDALASVVA
                     AHENLGSEHYQELNQFGDGSFTGEMLSNSDQASVYFAFDTGGSAPLFQTVSGSNYSYG
                     AIFEYGTPAAATLLQTVRVDSLVGLFTGWSLAPDTRHSLATFARQDINTVPGGTADDM
                     TFSIDGPAATLGSISVPEAMGTGGIDSNLSGNCDTDYFQFTTPVSFDTATGVTIAASF
                     SGGLDGVISVFNTSGTQIARADTFGVNGGETLTTALAASTTYVVRVGSFNGTTSGSYK
                     LRIRAPKTLSNGMSNLGG"
BASE COUNT          750 a         1403 c         1452 g          882 t
ORIGIN      
        1 aactgctttt aagccggtat gcgcgcctta cggcccgccg cggcgggccg cgtcggcatc
       61 ggcggccaga ccgcgcatgt aagcgaagaa tcccgccgac tggccgcgga atatgcccat
      121 ggccgtgaga tacgccagcg cgcccttggc cgcccctttg gtctccgtgg gccgaacaat
      181 tgaacagatt ggcaagtctg tgctcaggaa cgggcaaggc tccggcatga tcaggttcga
      241 ctggagcagt tccccagcgt ttgacaggtc tggaaacgtc aggccattgg tggggtcggt
      301 tagcggcggc gcatttcccg ccttgtcctg ccaggtctgg aagtgcatcg cctcggtcgg
      361 cccgatgctg agcacaaccc gcagtgcttc ggcgccggtg atccgctgcg cgaggctggg
      421 ataaaggctg gtgccgccca cttcgaccga cggaaagtgg aacccggcgg tgttggcgat
      481 ggcctgaaga tgcttggcgg gcgcgaggtc ggcatcggtg cggggaatgc cgggaaactg
      541 gccattgtga agatccggca ccgcctgggg aaagatgaaa tccggatcca ggtcggggtt
      601 gtcgcttgga tcgcgatagc gcgaccacca actggtgtcc agcgtgagcc gcatgagatt
      661 cgtcaaccgc ccaatctgct gagcgccggt cgcctggctg ctcggcaagg tgcggaatcg
      721 ttcgagatcg gccggctccg ccccgtgcgc ctcaaggtag gcgttgagga acagctcgtg
      781 ggtcatctca ttttcagtgt tatcgtggat gtactgcgcc atgtcctcat cgagaaccgc
      841 cagcgcctcg gtataagcgg ggttcccgct tccgccggga acttcatcgt cctggatgcc
      901 gcccagctcg ttgtactgtt gccagagatc ggtctcgagg atctcggcgg cggcgaggaa
      961 tcgcaggatc gcggcatcac ctcgggtgag cctgccgtgc tcccggtcgt cgtcccggtc
     1021 attgtcacgg tccttcttgg cctgcgccgt ttcgccggcc agcgcgacga cccctgcgac
     1081 cgctgccgtc ctgagcagtg tcctgcgtcc gaccatcgcc tgtggagtga ttaacttctc
     1141 ggtcatggca gttcccttct gcgcttcgga tgttcccatc cgcttgagcg cgttaaatgt
     1201 gatgatgcca agtatgaatc gagaggacgc gcgcgtgggc ggttaagggg gcgcagagga
     1261 tcgaacaccg aaccgatcga cggcggccga acacagcctc ggctccgccc aacccactgg
     1321 tttttcttcg gcctccaaaa aggccgcgtg cggcaagggg cgcaacctta atcgccgctg
     1381 cgcgctttct tgcccattaa tgcacctttt gcagcagctt ttttccaatg ggcagatctt
     1441 ggcgccgcgc cgccgctcgt cctctgcggg caggattcgg catgtgcctg attacgttgc
     1501 cgacggcact ttccagcccc gccgccccga cggtcgaggc gtgcttcgcg attcagactc
     1561 tcgccttcta cgcgctttcg tccggcccgc cgggttgctt tgcgcaaggt caattagctt
     1621 ggccttacaa ccgctgaagt tctgactgtg ttgcttcagt atctttgaaa tggattgccg
     1681 tgaatttgat tgcgggttct tgccgggcat gatagcgttt cgccccgtcg ggtgcgccgc
     1741 ggctggttcg tgcgggattc aattccagtt gttattgtag gtcgggttct gccgttcgct
     1801 cacatcgtgt gtctgttcag cacctctgcg cgcgctcgct cgtttagcct cggggagaga
     1861 atcacatgcg cctgcacaat gtgcgtcagt ctgatatcgc gtcggccggt ttggttctgc
     1921 gtcgccgccg ggcgatgttg gcggaagcgc tggagcagcg gctgctgttt gcgagcctgc
     1981 gcgacttcgt cgggctttca gccgagacgt cttcgcggtt gctgggcttc gtctccacgg
     2041 cgcacggtgg atcggggcag cagcagcagc agacggtggt ggccggcgac gggttgaccg
     2101 acagcgtgtg gctggcgaat cacgttgatc tgccgacaat cgcgttgctg gataaggtgg
     2161 acgggccgtt gcagcaaatg gtcgcgcagt cgctgcggga cgggaaatcg ttcgcggaca
     2221 tgtcgatcgg gaacgccccg ccatcggatc agtacgagca cgcgggtgac gatccgctgg
     2281 tgacgatccg atcggcggag ccgacggcgc tggcggcgaa gctgcgagcg ctgggcttca
     2341 acgtcagcgg cagcgtggag aacggcgagg cgggcgtggt ggacgtgtac acgccggtct
     2401 cgcgcatcac ggagctggcg ggcgtggcgg gtgtgaagtc gcttggcgcc gagcagaacc
     2461 tgccgaccga tgccggttcg atcagcagca cctggccggg cacggcgaac attccgcagt
     2521 tgctgctgga ccgcccgggc atgaccggcg cgggcatcaa atacggcatc atcagcgaca
     2581 gcttcaacaa cctgggcggc atggccgcgg gcattgccac cggtgacctg ccggccggtg
     2641 tgacgatcct gcacgacgcc gccggcggtg gcaccgatga aggccgcgcg atggccgagg
     2701 tgctttacga cgccgcgccc ggctcgaaca tcctgttcta tgactccggt gggtcaaaaa
     2761 cgggcatggt cacggcgatc aacgcgctgt cggcggccgg ggcgaacgtg atcgttgatg
     2821 atgtgaacga ggttgaggga acgttccagg acgacgtgct ttcgcaggcg atcgacgccg
     2881 ttgtactgaa caagaatatc ccttacttca cgtcgtcggg gaatcgcaat gggcagactt
     2941 ggtatggcgc gttcaacgaa gggatcgttg tcgacagcta taacgcgttt gacggcgcgg
     3001 ccatccagaa tctgttagtc acgattccgg ccaacgggac catcacggtt gacctgtggt
     3061 ggggccaaaa gctgggcggc gcgacgaccg acctcgatac gtttctgaac gacgccaaca
     3121 ccggcacctc gctggccagc gccaccggcg tcgagatcgg cggcgacgcg ttcaccggtt
     3181 tcggctatac caacaacact ggcgcgacga tcaccggcag catctctctg cgcaaagcag
     3241 gcgggacgtc agcgatcggc ctaccgttgt tcctgctcac cttccccggc aagggcacgg
     3301 ctcccaccgg tcccttctgg ggcgggccgg ctgcttacgg gcatcacatg tcggagttcg
     3361 gattcggcgt cgccgcggtg cagccggcga gtccgaacac gatcgagtcg ttcagctctc
     3421 ttggtggcgt tccgatcctg ttcaatgacg acggcaccgc cgtcagcggt ggcagcgtga
     3481 ttcgcaatca acccgcgctg gccgcgtgcg atgggatcaa caccactttc ttcggccagt
     3541 tcttcggcac cagctgtgcc gcgcccgacg ccgccgccat tgccgggctg ctgttgcaag
     3601 ccgccggtgg tggtggaacc ctgacctact cgcagatgcg cgacatgctg agcaacagcg
     3661 cgttcgacct gggctcggcc ggatatgacc tgacctacgg cgccggccgc atcgacgcgc
     3721 tcgcctccgt cgtcgccgcc catgaaaacc tgggcagcga acactatcag gaattgaatc
     3781 aattcggcga tggatcgttc accggcgaga tgctttccaa ttccgaccag gcgtcggtct
     3841 acttcgcgtt cgacaccggc gggtcggctc cgctgttcca gaccgtctcg ggttccaact
     3901 attcgtatgg cgcgatattc gagtacggca cccccgcggc cgcgacgctg ttgcagaccg
     3961 tgcgcgtcga ttcgctcgtc ggcctgttta ccggttggag cttggctccg gacacacgcc
     4021 atagcctggc caccttcgcc cgccaggaca tcaacaccgt tcccggtggc accgcggacg
     4081 acatgacctt ctcgatcgac ggccccgcgg cgacgctcgg ctcgatcagc gtgcccgaag
     4141 ccatgggtac cggcggcatc gacagcaatc tatccggcaa ttgcgacacc gattacttcc
     4201 agttcacgac accggtgtcg ttcgacaccg caaccggcgt cacgatcgcc gcatcgttca
     4261 gcggtgggtt ggacggcgtg atcagcgtct tcaacaccag tggcacgcaa atcgcgcgcg
     4321 ccgacacctt cggtgtcaat ggcggggaaa cgctcaccac ggcactggca gccagcacaa
     4381 cgtacgtggt gcgcgtcggc tcatttaacg gtactacgtc cggctcgtac aagttacgca
     4441 tccgcgcgcc caagacgctg tccaacggca tgtcgaacct gggcggc
//
LOCUS       DATIMH010001088         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4302490, whole genome shotgun sequence.
ACCESSION   DATIMH010001088 DATIMH010000000
VERSION     DATIMH010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4302490"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VLI90_18800"
     CDS             complement(<1..561)
                     /locus_tag="VLI90_18800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV16320.1"
                     /translation="MHSELLSEVAGQAEVTPAIVAFHDVSKWYGNVIGLNKLTIRIPA
                     GVTGLLGPNGAGKSTLLQLATGQLYPSQGSVQVLGQPIWNNPSLNRSIGLCPEQDAFY
                     EWMSGFDFVMTCARLSGMSKRAARDAAGRTIESVGMSRHKDRAIRGYSKGMRQRTKMA
                     QALVHDPEVLFLDEPMTGTDPVARRDL"
     gene            complement(516..1664)
                     /locus_tag="VLI90_18805"
     CDS             complement(516..1664)
                     /locus_tag="VLI90_18805"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSV16321.1"
                     /translation="MPIFDQGYQHWSGELSGHAWRWLAISRQGVRVGLKNRFLRLLLF
                     VAWVPALALVLMLCAWGLLERGSAMVSGFTRLIALMQPSLVMDPKYYRADIWRLSYGY
                     FLQIELWVSMILVLLAGPSLISQDLRFNALPLYFSRPLRRIDYFLGKLGVIVAFLAMV
                     VILPAIVAYIFGLLFSLDITILRDTFGILLASIGYGVIIALSAGLLILALSTLSRNSR
                     YVALLWIGLWFVGSIVGHALQAVDSQDRRQAYYNKITVTRPMGRDDASLSNAERQRQR
                     REWHRAIQRANDEYRVAELEFSKNDWRPLVSYSANLSRLGQQLLHTNQTWAKLAAMQP
                     DPASRDRILALYMGPQFPWYWSAGVLVGLFGLSACILNFSVKSLDKLK"
     gene            complement(1665..2573)
                     /locus_tag="VLI90_18810"
     CDS             complement(1665..2573)
                     /locus_tag="VLI90_18810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV16322.1"
                     /translation="MLFELEGVTKTYGAVTALDDLSLSVAPGAIGLLGPNGSGKTTMI
                     RTLLGLIQIDAGSGNVLGMDIRRRQLDVRRAVGFAPEDECLFPRMEGIEFVAYAGELV
                     GMRTKDAMQRSHEVLDYVGLGEVRYRKVETYSTGMKQRLKLASAIVHDPDLLILDEPT
                     NGMDPAGREEILQLSRDLAHNKGMSLLFSSHLLPDVEAVCDTIVVLGGGKLLAQGDIQ
                     QLKELHNRFFEVRVKADAELFAQRLSALGCSTEIREDIILVQLPEGRTPQMLWQVAAD
                     HRQQIRHLRPQRGTLEEVFLKAVERT"
     gene            complement(2761..>3467)
                     /locus_tag="VLI90_18815"
     CDS             complement(2761..>3467)
                     /locus_tag="VLI90_18815"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HSV16323.1"
                     /translation="PESVKAALSGVDKLFLLVGNVADELTQALLTNAVAREAKIKHVT
                     YLSVFGAERFPGVPHFIGKQTVENAIKSFDTPFSILRPGYFFQNDARFKDLLTGPGVF
                     PLPIGTVGVGAVDVRDIADAAAITLTTDGHAGKTYNLVSHSILTGLGAAEVWSKALGK
                     PVIYPGMPLEDFERQLRQMVPAWLAMDMRLMFQIYQEQGNVPAAGDADALSKLLGHAP
                     RRYEDFVRQTVAAWRA"
BASE COUNT          678 a         1146 c         1035 g          608 t
ORIGIN      
        1 caggtcgcgc cgcgcgacgg gatcggtgcc ggtcatcggc tcgtccagga acaggacctc
       61 ggggtcgtgc accagcgcct gcgccatctt ggtgcgctgc cgcatgcctt tggaatagcc
      121 gcggatcgcg cggtccttgt gcctgctcat gccgaccgat tcgatcgtgc gccccgccgc
      181 gtcgcgtgcc gctcgcttgc tcatgccgct gaggcgcgcg cacgtcatca cgaagtcgaa
      241 gccgctcatc cattcgtaaa acgcgtcctg ttccggacac agtccgattg atcggttgag
      301 cgatgggttg ttccagatcg gctggccgag cacctgcacg ctgccctggc tgggatacag
      361 ttgcccggtg gccaattgaa gcaacgtgga cttgcccgcg ccgttcggcc cgagcaaacc
      421 ggtcaccccc gcggggatgc ggatcgtcag cttattgagg ccgatcacgt tgccgtacca
      481 cttcgaaacg tcatggaagg cgacgatcgc cggcgtcact tcagcttgtc cagcgacttc
      541 actgagaagt tcagaatgca tgccgacaat ccaaagaggc cgaccaacac gccggccgac
      601 caataccacg gaaactgcgg acccatgtac agcgcgagga tgcgatcgcg actggcgggg
      661 tccggttgca tcgccgccag cttcgcccac gtttggttcg tgtgcagcag ctgctggccg
      721 aggcgcgaca ggttcgccga gtacgacacc agtggccgcc agtcgttctt cgagaactcc
      781 agctccgcca cgcgatattc atcattggcg cgctgaatcg cccggtgcca ctcgcggcgc
      841 tggcgctgac gttctgcgtt ggacagcgaa gcatcgtcgc gccccatcgg ccgggtcacg
      901 gtgatcttgt tgtagtacgc ctgccgccga tcctggctgt ccaccgcctg cagcgcgtga
      961 ccgacgatgc tgccgacgaa ccagaggccg atccacagca gcgcgacgta acgcgaattg
     1021 cgcgacagcg tggacagcgc caggatcaac agccccgctg acaaggcgat gatcaccccg
     1081 tacccgatgc tggccagcag gatgccgaac gtgtcgcgca ggatcgtgat gtcgaggctg
     1141 aacagcaggc cgaagatgta cgcgacgatc gcgggtagga tgacgaccat cgccaggaag
     1201 gcgacgatga cgccgagctt gcccaggaag taatcgatcc gccgcagcgg gcgcgagaaa
     1261 tacagcggca gcgcgttgaa gcgcaggtcc tggctgatga ggctcggccc ggccagcagg
     1321 accaggatca tcgagaccca caattcgatt tgcagaaagt aaccgtacga cagccgccag
     1381 atgtcggcgc ggtaatattt cggatccatc accaagctgg gctgcatgag ggcgatcagc
     1441 cgcgtgaagc ccgagaccat tgccgagccg cgctcgagca atccccacgc gcacagcatc
     1501 aacaccagcg ccagcgccgg cacccacgcg acgaacaaaa gtaatcgtag gaaccggttc
     1561 ttaagaccca cgcgaacgcc ctgccggctg atcgccagcc agcgccacgc gtgccccgac
     1621 agctcgccgg accaatgctg atagccctga tcgaagatcg gcatttacgt ccgctccacc
     1681 gctttgagga agacttcttc caacgtcccg cgctgcggcc gcaggtgccg gatctgctgc
     1741 cgatgatccg ccgccacctg ccaaagcatc tgcggcgttc gtccctcggg cagctgcacg
     1801 agaatgatgt cctcgcgaat ttccgtcgag caacccagcg cgctcaatcg ctgcgcgaac
     1861 agctccgcgt ccgctttcac gcgcacctcg aagaagcgat tgtgcagttc cttcagctgc
     1921 tgaatatccc cctgcgccag cagcttcccg ccgccgagga caacgatcgt atcacacacc
     1981 gcttcgacat ccggcagcag gtggctggaa aacagcaggc tcattccctt gttatgcgcc
     2041 aggtcgcgcg acagctgaag gatctcttcg cgccccgccg ggtccatgcc gttggtgggt
     2101 tcgtcgagga tcagcaggtc cgggtcgtgc acgattgccg acgccagctt gagccgctgc
     2161 ttcatgccgg tcgaataagt ctcgaccttg cgatatcgca cttcacccag cccgacataa
     2221 tcgagcactt cgtgcgaccg ctgcatcgcg tccttcgtcc gcatgcccac cagttccccg
     2281 gcatacgcga caaactcaat accttccatc cgcggaaaca gacactcatc ctccggcgcg
     2341 aagccgacag cgcgccgaac atccaattgc cgccggcgga tgtccatccc cagcacgttc
     2401 cccgaccccg cgtcgatctg gatgagcccg agcagcgtgc ggatcatggt cgtcttgccg
     2461 gacccgttag ggccgagcaa tccgatcgcg ccgggggcga cggacaacga aaggtcatcg
     2521 agcgccgtga cggcgccgta ggtcttggtg acgccttcaa gttcaaacag catgcgagta
     2581 gcccttgcgc aagacgcggc atcttatatg acggagcggc cgggtgctgc attcggcgaa
     2641 cacgacgggc aaaaaaacgc cgatcgggtt gtgctggccg ccactccctg tcgcctcgga
     2701 gtggattcgt gaggcgccgc ccggcgtgat ggccgttcag tatgcgatat tcgctggcct
     2761 tcacgcccgc caggcggcga cggtctggcg aacaaaatcc tcgtatcgcc ggggagcgtg
     2821 cccgagcagc ttcgacagcg catcggcgtc gccagcagcg ggaacgttgc cttgttcctg
     2881 gtagatttgg aacatgagcc gcatgtccat cgccagccag gccggcacca tttggcgaag
     2941 ttgtctctcg aaatcctcca acggcatccc cggatagatc acgggcttgc caagggcttt
     3001 gctccagacc tcggcggcac ccagaccggt cagaatcgaa tgactgacga ggttgtacgt
     3061 ttttcccgcg tgcccgtcgg tcgtcaaggt gatcgcggcc gcatccgcga tgtcgcgaac
     3121 gtcgacggcg ccaaccccaa ctgtgccgat cggcaacggg aacacgcccg ggccggtcag
     3181 cagatcctta aatcgggcat cattctggaa gaaatagccc ggccgcagga tggaaaacgg
     3241 cgtgtcgaac gatttgatcg cattttcgac cgtctgcttt ccaatgaagt gaggcacacc
     3301 cgggaaccgc tcggccccaa ataccgagag gtacgtcacg tgcttgatct tcgcttcgcg
     3361 agccacggcg ttggtgagca gcgcttgagt caactcgtcg gcgacgtttc caacgagcag
     3421 gaatagctta tcgaccccgc tcagggcagc tttgaccgac tccggat
//
LOCUS       DATIMH010001089         4721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2279013, whole genome shotgun sequence.
ACCESSION   DATIMH010001089 DATIMH010000000
VERSION     DATIMH010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2279013"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..3758)
                     /locus_tag="VLI90_18820"
     CDS             complement(219..3758)
                     /locus_tag="VLI90_18820"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HSV16324.1"
                     /translation="MKHVIVSTGVSAGVAGSSRAVGERSSAPMAPPPLAASQTRWRGL
                     VCEAAIGDGGGAILAILLLAILPTLARAGNDKLIPLFDGKSLTGWHQAGPPGFDLKDG
                     ILTAHGGMGLLWNDRELGDFILRLEFQVSKKEDNSGVFVRFPDPGNDPWIAVNQGHEI
                     QICDTEANNRTGAIYNFKDASELATKPVGEWNAMEIKVVGRSYTVTINKKVVNEYTST
                     TRPLRGHIGLQNHWGAVSFRNITVEELTGGAAAVAAGQPVSLKDASPGLVGEYFENIA
                     GFKALTQVKQREPFFLRVDPQINFAAVEGEFYGSHLIENFGVRWTGAIHIDKPGTYTF
                     DLRSDDGSKLTINGKTVVDHSRAHGMEDKAGQVRFTTAGDYPLKLEYNQFASAAGCIF
                     NWQPPNEKDLSVVPASVLVHEKSAEDIQWNQADWKLAQTDHRAWIKKHGRKFEKMDYG
                     PFLAGTFVAPQPKDNTTLKGIIVKLNDEGTANVLFDTELMRYSAGWTGDFIDYHGVAY
                     DGAHGVDPKIKGEQVFGTPSTPGWEHEGAWNDPRSTPYGPLPHEWAHYKGLYRNADRV
                     IFSYTINGSDVLEMPHAESHDGAMMFVRSFQIGPCKSPLTMLVCQQPSAANQVEVFGT
                     SNLKIATDSSGRVTATFPPQDATASYQVSISSARRQTPAKMVEIVQQVGGSPLDSLCK
                     GGPRLWGEPIVTQGALGSGDGPYVVDTITSPENNPYDAWMRLGGFDFFPDGHRAAVCT
                     WNGDVWIVSGIDDSLQHLTWQRFATGLFQSLGLKIVNNEIYVLGRDQITRLHDLNGDG
                     EADFYENFNNDCQVSPGFHEFAHDLQVDSQGNFYYAKGGPVNSGGRGFEKLTDHAGTI
                     RKVSPDGKTSETFATGVRAPNGMSMGPHDEITVSDNQGTWVPACRISFVTRGAFLGVP
                     DTAHLDPKPTNYGNPICWFPYVEGDYTTGDNSSGGAAWAPLDDTKWGPFAGQPIHLSY
                     GTCSLFKLMYENVDGVWQGGVVRFPLDFDSGIMRARFNPVDGQLYVCGLKGWQTRATK
                     DGIFQRVRYTGKTVTIPTELHVSGDSVSVVFTNPVDPESARDSGNYDVEQWNYIWSDD
                     YGSPEVSRDDPKKKGRDTVDIDSIEIGKDNRTVRLKIADLKPVMQMKIKMKIKAADGT
                     PLDYEIDSTINKIPGGPSIATPQPPAEAHAALK"
     gene            complement(3758..>4721)
                     /locus_tag="VLI90_18825"
     CDS             complement(3758..>4721)
                     /locus_tag="VLI90_18825"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSV16325.1"
                     /translation="VLGTFALLTVAMFLSPRTQQARAADGALRPKIGVSIPAADHGWT
                     AGIGWWAKRAMALHPEIDWVYQTAQNPEKQNADIENMLAQNVDGLVILATESAPVTPV
                     AKQAHDRGVFIVNVDRGFTEPVADLFLEGDNKAFGRKSAEYIARRLNGKGNIVILEGI
                     PSTVNSDRVNAAKEVFKQHPDIKILGDQPGMWNREKALEIMQNFLTAFPKIDAVWAQD
                     DDMALGAIQAIKEAGRDKQIWVFGGAGMKDVVKMVMDSDPMVPADITYPPSMIAAGIH
                     EAASVLRDGKRKQVQQFLPKHLMLDVELITPENAKEYYFPDSVY"
BASE COUNT          821 a         1474 c         1421 g         1005 t
ORIGIN      
        1 ggctgacgca aatgacacaa tcagaccagt catgagcttc gccggtttta tcgcaggagc
       61 atcggcggca aacgaggaat gacgaagcgg cagctgcgca gcaagcacac agctcacaac
      121 ggcagcgatg aacatccagc gtcgatcgat tcgtttcata tgtagcacgg gcgtctcgcc
      181 cgtgtctgga gccgttcggc acgggcgaga cgcccgtgct acttcagtgc cgcgtgcgcc
      241 tcagccggcg gttgcggcgt ggcgatcgac ggcccgccag gaatcttgtt gatcgtgctg
      301 tcgatctcat aatccagcgg cgtgccgtcg gcggctttga tcttcatctt gatcttcatc
      361 tgcatcaccg gcttcaggtc cgcaatcttc agtcgaacgg tccgattgtc cttgccgatc
      421 tcaatcgaat cgatatccac cgtgtcccgg cccttctttt tcgggtcgtc gcgcgacact
      481 tcgggtgagc cgtaatcatc cgaccagatg tagttccatt gctcgacgtc atagttgccg
      541 gagtcgcgcg ccgattcagg atcgaccgga ttggtgaaga cgaccgaaac gctgtcgccg
      601 ctaacatgca gttccgtcgg gatggtcacg gtcttcccgg tgtagcgcac gcgctgaaag
      661 ataccgtcct tcgtcgcgcg ggtttgccag cctttcagcc cacatacgta cagctgacca
      721 tccacggggt tgaagcgcgc ccgcatgatg ccggaatcaa agtccagcgg aaatcgcacg
      781 acgccgccct gccagacgcc gtccacgttc tcgtacatca gcttgaagag cgagcacgtg
      841 ccatacgaca ggtggattgg ttggcccgca aacggtcccc acttcgtgtc gtccagcggc
      901 gcccacgcgg ctccgccgct ggagttgtcg ccagtggtgt aatcgccttc gacgtacggg
      961 aaccagcaga ttgggttgcc gtagttcgtc ggcttgggat cgagatgcgc tgtatcaggt
     1021 acgccgagga atgcgcccct ggtcacaaaa ctgatccggc acgcgggcac ccacgtgccc
     1081 tggttatcgc tgacagtgat ctcgtcatgc gggcccatgc tcatgccgtt gggcgcgcgc
     1141 acgccggtgg cgaacgtctc cgacgtcttc ccatccggcg acactttgcg gatcgtcccc
     1201 gcgtgatcgg tcagcttctc aaacccgcga ccgccggagt tcaccgggcc gcccttggca
     1261 taataaaagt tgccctgcga atcgacctgc aggtcgtgcg cgaactcgtg gaaccccggc
     1321 gagacctggc agtcattgtt gaagttctcg taaaagtccg cttcgccgtc gccgtttaga
     1381 tcgtgcagcc gcgtgatctg atcgcggccg aggacgtaga tctcgttgtt gacgatcttg
     1441 agacccagcg attggaacag ccccgtggcg aagcgctgcc aggtgagatg ctgcagcgaa
     1501 tcatcgatgc cgctgacgat ccacacgtcg ccgttccacg tgcagacggc ggcacgatga
     1561 ccatcgggga agaagtcaaa gccgccgagg cgcatccacg catcgtacgg gttgttctcc
     1621 ggcgacgtga tggtatcgac gacgtacggg ccgtcgccgc tgccgagcgc accttgcgtg
     1681 acgatcggct caccccacag ccgcgggccg cccttgcaaa gggagtccag aggactgccc
     1741 cccacctgct gcacaatctc caccatttta gcgggcgtct gacgccgggc tgacgagata
     1801 ctaacttgat agctggcggt tgcatcctgt ggcggaaagg tcgcggtgac cctgccgctg
     1861 ctgtcggtgg caattttgag attgcttgtt ccgaacacct ccacctggtt cgctgccgat
     1921 ggctgctgac aaaccagcat cgtcaaagga gatttgcacg gaccgatttg gaatgaccgg
     1981 acgaacatca tcgcgccgtc gtgtgattcc gcatgaggca tttcgagaac gtcggagcca
     2041 ttaattgtgt aagagaagat gacgcgatct gcatttcgat agagaccctt gtaatgcgcc
     2101 cattcgtggg gcagcgggcc atacggagtt gaacgtggat cgttccacgc gccctcatgt
     2161 tcccagcctg gcgtcgaagg tgttccgaag acctgttcgc ccttgatctt cgggtccacc
     2221 ccgtgcgccc cgtcatacgc cacgccgtgg tagtcgatga aatcgccggt ccagccggcg
     2281 gagtatctca tcagctccgt atcgaacagg acgttcgccg tgccctcgtc gttcagcttg
     2341 acgatgatgc ccttcagcgt cgtgttgtcc ttcggctgcg gcgcaacgaa cgtgccggcg
     2401 aggaacgggc cgtagtccat cttctcgaac ttccgcccgt gctttttgat ccacgcgcgg
     2461 tgatccgtct gcgccaattt ccaatctgcc tggttccact gaatgtcttc agccgacttc
     2521 tcgtgcacca gcaccgacgc cgggacgacc gacaaatcct tctcattcgg cggctgccaa
     2581 ttaaagatgc atcctgcggc gctagcgaat tggttgtatt ccagcttgag cgggtaatcc
     2641 cccgctgtgg tgaagcgaac ctgccccgcc ttgtcttcca tgccatgggc gcggctgtgg
     2701 tcgacaaccg tcttgccgtt gatcgtcagc ttggagccgt catcgctgcg cagatcgaac
     2761 gtgtacgtgc cgggcttgtc gatgtggatc gcgccggtcc agcgcacgcc gaagttttcg
     2821 atcaggtgcg acccgtagaa ttccccttcg accgcggcga agttgatctg cggatcgacg
     2881 cgcaggaaga acggctcgcg ctgcttgacc tgcgtcagcg ctttgaaccc tgcgatgttc
     2941 tcgaaatact cgccgacgag gcccggcgac gcgtctttca atgacaccgg ttgccccgcg
     3001 gcgacggccg ccgcgccacc ggtcaattct tcgaccgtga tgtttcggaa gctgacggcg
     3061 ccccagtggt tttgtaagcc gatgtgtccc cgcagcgggc gcgtggtgga cgtgtattcg
     3121 ttgaccacct tcttgttgat cgtcaccgtg tacgaccggc cgaccacctt gatctccatc
     3181 gcgttccact caccgacggg cttggtcgct agctcgctgg cgtctttgaa attataaatc
     3241 gcgccggtgc gattgttcgc ttccgtgtcg caaatctgga tctcgtggcc ctggttcacc
     3301 gcgatccacg ggtcgttgcc cgggtcggga aagcgcacga agacgccgga gttgtcttcc
     3361 tttttcgaga cttggaattc gaggcggaga atgaaatcgc ccagttcgcg atcgttccag
     3421 aggagaccca tgccgccgtg ggcggtgagg atgccgtcct ttaagtcgaa gcccggcggg
     3481 ccggcctggt gccagccggt gagggatttg ccgtcgaaga gggggatcag cttgtcgttg
     3541 ccggctcggg cgagggttgg gaggatcgct agaagcagga tagcgaggat cgcgccgccg
     3601 ccgtcgccga ttgcggcttc gcaaaccaga ccgcgccacc tggtttgcga agccgcaagc
     3661 ggcggcggcg ccatcggcgc gctggatcgt tcaccgactg ctcggctcga tcctgcgact
     3721 ccggcggata cgcctgtgct gacgatgaca tgcttcatca atacaccgaa tccgggaaat
     3781 aatattcctt tgcattctcc ggcgtgatca gctcgacatc gagcatcagg tgcttcggca
     3841 ggaactgctg cacctgcttt cgcttgccgt cgcgcagcac cgatgccgcc tcgtgaatgc
     3901 cggcagcgat catgctcggc ggataggtga tgtcggccgg caccatcggg tcgctgtcca
     3961 tcaccatctt cacgacgtcc ttcatcccgg cgccgccgaa gacccagatc tgcttgtcgc
     4021 gccccgcttc cttgatggcc tggattgcgc ccaaggccat gtcgtcgtcc tgcgcccaca
     4081 ccgcgtcgat cttggggaat gcggtgagga aattctgcat gatctccagc gccttctcgc
     4141 ggttccacat gcccggctgg tcgccgagga tcttgatgtc cgggtgctgc ttgaacactt
     4201 ccttggcggc gtttacgcga tcgctgttca ccgtgctggg gatgccttcg aggatgacga
     4261 tgttcccctt gccgttcaat cgcctggcga tgtactcggc gctctttcgg ccgaacgctt
     4321 tgttgtcgcc ttcgagaaac agatcggcca ccggttcggt gaacccgcga tcaacgttga
     4381 cgataaacac cccgcggtcg tgcgcctgct tggcgactgg cgtcaccggg gcgctttcgg
     4441 tcgcgaggat caccagcccg tccacgttct gggccagcat gttttcgatg tcggcgttct
     4501 gcttctcggg gttctgcgcg gtttgataga cccagtcgat ctccggatgc agcgccatcg
     4561 cgcgcttggc ccaccagccg atgccagcgg tccaaccatg atcggcggcg ggaatggaga
     4621 cgccgatctt cggacgcagc gcgccgtcag ctgcgcgcgc ctgttgcgtg cgcggcgaga
     4681 ggaacatcgc caccgtgagc aatgcgaatg tgccgaggac a
//
LOCUS       DATIMH010001090         1979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5173763, whole genome shotgun sequence.
ACCESSION   DATIMH010001090 DATIMH010000000
VERSION     DATIMH010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5173763"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VLI90_18830"
     CDS             <1..1107
                     /locus_tag="VLI90_18830"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HSV16326.1"
                     /translation="AAILIVAAATFWTKGMVVSDIGEAHQMLHRLLGTKVAPLAFALA
                     LICAGQSSTITGTLAGQITMEGFLHFRMRPWLRRLITRSLAIIPAIVVISIKGEGGTM
                     GLLVLSQVVLSLQLPFAVVPLVKFTSSRFRMQRFVTPIWLAVCAWVVAVIIIGLNGKM
                     SFDQIVEWANAAGPHRWAVLATATPVAVALAALLTWMTFRPDRAPEEARTVSADDVVN
                     SASEMVRIVRRVGVAVEGTADDAPMVAEAMALARSHRAELVLMHIVEGVGGQWHGPGA
                     GDQEYHLDEKYLNALVARMNLELSGSGAPPARMTLGYGSVPKELVRLARENSVDLLLV
                     GGHGHRGLNDILRGTTIDAVRHGLKIPILAIRGK"
     gene            complement(1335..>1979)
                     /locus_tag="VLI90_18835"
     CDS             complement(1335..>1979)
                     /locus_tag="VLI90_18835"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HSV16327.1"
                     /translation="WSLNVADKYTTLFIVRPDDGRYAFASVGWPGMIGVLSGMNEHGL
                     SLANMEVTREPRLPHAMPYTILYRTILEQCRTVDEAVALLQKTPIQTANNLMLMDAAG
                     NRAVAELSPDTGVHIRRGLPEHVLISTNHQRDQDQDTAGRCWRYDYIHQTAAAEFGHV
                     DLAAIESMLAHTGSKITLQSMVFEPANRVIYLSAGTDAAHHEYSRLDLRPYFKR"
BASE COUNT          341 a          525 c          733 g          380 t
ORIGIN      
        1 gcagcgattc tgatcgtcgc cgcggcgacg ttctggacga aggggatggt ggtgagcgac
       61 atcggtgaag cgcatcagat gcttcatcgg ctgctcggaa cgaaagtcgc gccgctggcg
      121 tttgcgctgg cgctgatctg cgcggggcaa agctcgacga tcaccggcac attggccggg
      181 cagatcacga tggaaggatt cctgcacttc cgcatgcggc catggctgcg gcggttgatc
      241 acgcgcagcc tggcgatcat cccggcgatc gtcgtgatct cgatcaaagg tgaaggcggg
      301 acgatggggt tgctggtgtt gagccaggtg gtcctgagcc tgcaactgcc gttcgcggtg
      361 gtgccgctgg tgaagttcac cagcagccga tttcgcatgc agcggtttgt cacgccgatc
      421 tggctggcgg tgtgcgcgtg ggtggtggcg gtgatcatca tcggtttgaa cgggaagatg
      481 tcgttcgatc agatcgtcga atgggcgaac gcggcggggc cgcatcggtg ggcggtgctg
      541 gcgacggcga cgccggtggc ggtggcgctg gcggcgctgc tgacgtggat gacgttccgg
      601 ccggaccggg cgccggagga agcgcggacg gtttcggcgg acgacgtggt caattccgcg
      661 agcgaaatgg tccgcatcgt gcggcgcgtc ggcgtcgctg tggaaggaac ggccgacgac
      721 gcgccgatgg tggccgaggc catggcgctg gcgcgcagcc atcgcgcgga gctggtgctg
      781 atgcacatcg tcgaaggcgt gggcgggcag tggcacgggc cgggggcggg cgatcaggaa
      841 tatcacctgg acgaaaagta tttgaacgcg ctggtggcgc ggatgaatct ggaactgagc
      901 ggatcaggcg ccccaccggc gcggatgacg ctgggctatg gcagcgtgcc gaaggagctg
      961 gtgcgattgg cgcgggagaa cagcgtcgat ctgctgctgg tcggcggaca cggtcatcga
     1021 gggttgaacg acatcctgcg cggcaccacg attgacgcgg tgcggcatgg gctgaagatt
     1081 ccgattctgg cgatccgggg gaagtgattt ggtctctccg tctcgtttag ccgctgcgta
     1141 gacccggccg ctaaacgagg tggctatctt gtaaacatgg gtatcatggg atggatgggg
     1201 gcaaaatcga atgaagcgca acattgagcg ccgattcttg cgcgcatgca gcagcgcaaa
     1261 tgactgctgt atattgactt gtagcgtatt atcgacgcca aaacacatca aaattttttg
     1321 ttgcgcagct cgaatcatcg cttgaaataa ggccgcaggt cgaggcgact gtactcgtgg
     1381 tgggcggcat cggtgccggc ggagagatag atcactcgat tcgcaggctc gaagaccatc
     1441 gactgcaggg tgatcttgct gccggtgtgt gcgagcatcg attcgatcgc ggcgagatcg
     1501 acgtggccaa attcggcggc ggcggtttga tggatgtagt cgtagcgcca gcagcggccg
     1561 gcggtgtcct ggtcctggtc gcgctggtga ttggtgctga tcagcacgtg ctcgggcagg
     1621 ccgcggcgaa tgtgcacgcc ggtgtcgggc gacagctcgg ccaccgcgcg attgccggcg
     1681 gcgtccatca gcatcaggtt gttcgcggtt tgaatcggcg tcttctggag cagcgccacc
     1741 gcttcatcga ccgtgcggca ttgctcgagg atcgtgcgat acaggatcgt gtacggcatc
     1801 gcgtgcggca ggcgcggctc gcgcgtgacc tccatgttcg ccagtgacag gccgtgctcg
     1861 ttcatgccgg acaggacgcc gatcatgccg ggccaaccga ccgatgcgaa ggcgtagcgg
     1921 ccgtcgtcgg ggcggacgat gaacagcgtc gtgtatttgt cggcgacgtt cagcgacca
//
LOCUS       DATIMH010001091         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3725447, whole genome shotgun sequence.
ACCESSION   DATIMH010001091 DATIMH010000000
VERSION     DATIMH010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3725447"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VLI90_18840"
     CDS             complement(<1..381)
                     /locus_tag="VLI90_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16328.1"
                     /translation="MARANRRVPQICGAAVLSVCSTIIGFSTGDARAGNVILNGGYHA
                     QTTDHWCAAASLEMMLDVPAVRNNNAIVNQMLGVGDGPTVPSGTPDPTTTIVINNGVG
                     TITSRAQAFVYGLNHGLNTVNGIGY"
     gene            complement(619..>1697)
                     /gene="glnA"
                     /locus_tag="VLI90_18845"
     CDS             complement(619..>1697)
                     /gene="glnA"
                     /locus_tag="VLI90_18845"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HSV16329.1"
                     /translation="VAYLKKSKIADSARFGPELEFFIFDQAFYDQGINFSKHYVGSRE
                     GIWGRGDEDPTNLGYKVRLKEGYFPTPPMDTMQNIRSEMVAELINLGIPVEAHHHEVA
                     TGGQCEIDMRYQDLVTMADSVMMYKYVCKNVAARHGKVVTFMPKPLFQDNGSGMHVHF
                     SLWKGDKPLFAGNKYAGLSEMGLYAIGGILKHARALCALCNPTTNSYKRLVPGYEAPV
                     NLVYSSRNRSAAIRIPMYSENPKTKRIEFRCPDSSCNPYLAFSGMTMAAIDGIQNKIH
                     PGEPLDKDMYHLTSEEYERIGSAPGSLDEACDELERDHDFLLKGDVFTADVIHYWIKY
                     KRENEVDALRMRPHPYEFCMYFDI"
BASE COUNT          327 a          509 c          490 g          371 t
ORIGIN      
        1 gtaaccgatg ccgttgaccg tgttcagccc gtggttcaaa ccgtagacga acgcctgcgc
       61 ccgcgaggtg atcgtgccga cgccgttgtt gatgacgatg gtggtcgtcg gatccggcgt
      121 gccggatggc acggtcggtc cgtcacccac gccgagcatc tgattgacga tggcgttgtt
      181 gttccgcacc gccggcacgt cgagcatcat ttccagcgat gcggcggcgc accaatggtc
      241 ggtggtctgc gcgtgatacc cgccgttgag gatgacattg cccgcgcggg catccccggt
      301 tgaaaagcca atgatggttg aacagacgct cagaacagca gccccgcaaa tctgcgggac
      361 gcgacgattt gcacgcgcca tagtcctctc ccgttccccc ttaattgtcc tcagccagcc
      421 gcaaccaacc gaaatgatgg ggaaaggcta tagacagcag cacattattg tcaaattaat
      481 tctattttga gcagcacgct cccaattcgc cgactgtcgc cagggtctta gtgttaaaat
      541 gggcaaccgc gcctcatttt ccgaaacgcg cgcctcattt gaattcgcgc ggcggtgaag
      601 gaccggcaca cgccatcgtc aaatatcgaa gtacatgcag aactcatacg gatgcggccg
      661 catacgaagc gcatcgactt cgttctcgcg cttgtacttg atccagtagt gaatcacatc
      721 ggcggtgaag acgtcgccct tcaacaggaa gtcgtggtcg cgctccaact catcgcacgc
      781 ttcatccaac gaacccggcg ctgaaccgat gcgctcatac tcttcgctgg tcaggtgata
      841 catgtccttg tccagtggct cgccggggtg gatcttgttc tgaatgccgt cgatggccgc
      901 catcgtcatg ccgctgaagg cgagatatgg attgcaggaa gagtcggggc aacggaactc
      961 gatgcgcttg gtcttggggt tctcgctgta catcggaatg cgaatggcgg ccgatcggtt
     1021 gcgactgcta taaacgaggt tgacgggggc ttcgtatccc ggcaccaggc gcttgtagct
     1081 gttggtcgtg gggttgcaca acgcgcacag cgcgcgggcg tgcttcaaga tgccgccgat
     1141 cgcatataga cccatttcgc tgaggcccgc atatttattg cccgcgaaca gcggcttgtc
     1201 gcctttccac aagctgaagt gcacgtgcat gccgctgccg ttgtcctgga acagcggctt
     1261 gggcatgaac gtcaccacct tcccatgccg ggcggccacg ttcttgcaga cgtacttgta
     1321 catcatcacg ctgtcggcca tcgtcaccag gtcctggtag cgcatgtcga tctcgcactg
     1381 cccgccggtg gcgacttcgt gatgatgggc ttcgactggg atgcccaggt tgatcagctc
     1441 cgccaccatt tcgctgcgga tgttctgcat cgtgtccatc ggcggcgtcg gaaaatagcc
     1501 ttccttcaag cgcaccttgt aacccaggtt ggtcgggtcc tcgtcgccgc gcccccagat
     1561 gccttcgcgg ctgccgacgt agtgcttcga gaagttgatc ccttggtcgt aaaacgcctg
     1621 gtcgaagatg aagaactcca actcgggccc gaagcgcgcg gaatcggcga tcttgctctt
     1681 cttcaaatac gccaccg
//
LOCUS       DATIMH010001092         4996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2279365, whole genome shotgun sequence.
ACCESSION   DATIMH010001092 DATIMH010000000
VERSION     DATIMH010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2279365"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1403
                     /gene="opgC"
                     /locus_tag="VLI90_18850"
     CDS             108..1403
                     /gene="opgC"
                     /locus_tag="VLI90_18850"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HSV16330.1"
                     /translation="MGAFTLNTRDARLDFWRALCLIDMVLVHLVYEGVNFGPLQRALG
                     EYTRFAAGGFVFVAGLSIGAIFLPRTFRPNGRRTVYFSLWRRAFYILCVHYAATLSFV
                     SLDLIRGMRAEVPHLPPLMRDILLLREGGDLLPLYVILVAATPVVLDLLRRPRWRWLP
                     AVASVGLFAWGHAWEPALRFPMGQNFPVVLWQLMFTGGLLFGAALPRYNALATRTKTM
                     LAALACVISVVLWAADYRSDFGWAYPRLPLVFCKTPLSAGEALRYFGLTFTIIFSTDL
                     LWNRLNGNRVVKWVSPIGRKSLGCYVGHAWIVGVVGMIAHATTGAGAWQMMLAIPAVV
                     AVWLVAIALEWNSSVTKRIAPPTPTLSRWLPIPLACSAALVLFCGVGVIAARRMPPPA
                     AESQAIAIPDITDDDLDTNSNNGAPPSFDAADAADIIPT"
     gene            1406..2374
                     /gene="ribF"
                     /locus_tag="VLI90_18855"
     CDS             1406..2374
                     /gene="ribF"
                     /locus_tag="VLI90_18855"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HSV16331.1"
                     /translation="MQVHHGIDGLKSLPPGCVLSVGNFDGVHRGHRRLLEIANDLRGG
                     SGAGGVALVTFEPHPLTVLRPQLAPPRLTPLPLKQSLLEPLGVDHLIILPPTPDVLNL
                     TAEVFWQILRDDVRPTHMIEGESFNFGKGRGGSINRLREWSANTDVQLHVIGAVRVPL
                     LDLQVVAVNSSLIRWLLMHGRARDAAICLGRPYIIEGTVIQGHQRGRTIGVPTANLDC
                     GDQLVPAEGVYVGRCQIDGTTHAAAASIGTMPTFGENQLQVEVHVIDFSGDLYGQTLR
                     VELVDWVREQWRFADIEALKQRMAGDIKWSRRRFHAPVSEPFASLS"
     gene            2698..3696
                     /locus_tag="VLI90_18860"
     CDS             2698..3696
                     /locus_tag="VLI90_18860"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HSV16332.1"
                     /translation="MGASIGGMQAFQRICAALPADLPAAVFIVWHTALHSPGILPDVL
                     NRSARLPVAFARDGEAIEHGRVYVAPPDHHMLLWRDVVQLNRGPRQNRFRPAVDPLFH
                     SAADAFGPRVVGVIMTGGLDDGTAGLMHVKRERGVAVVQDPLDADEPGMPRSALEMVD
                     ADFVLPIAQIPEVLVRLATEQPQPPIRLEEAKAQMYNSDKDIGQGLRPEGVTGPPAEV
                     TCPDCSGALWRETEDGVVKYRCHVGHSFTEQGLLAAKNEEVELALWTALRVLKENATM
                     YRNIIERSTAAGRLASAEHFKQSLEQTEYELQVIKKALMTDSPGRAIRGERKSAPD"
     gene            4077..4928
                     /locus_tag="VLI90_18865"
     CDS             4077..4928
                     /locus_tag="VLI90_18865"
                     /inference="COORDINATES: protein motif:HMM:NF024870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSV16333.1"
                     /translation="MALRAKKPEAVIKRLKLFMYGPAGVGKTTAAIQFPNSYIIDAER
                     GAENYDKLVTASGSAVFQTNDINEVISEVKALLTERHDYKTLVIDPITTIYNDLLEKC
                     ELKVGTDFGRHYGEANKTMKRLANLIMALDMNVVVTAHAKTEYGSNLSKLGYTFDGWR
                     QLDYWFDLVVELGKKNKKRLAKVVKTRVESFPDDDIFEWSYEAIQKRYDISVLEKQAE
                     QVQLATPEQVREMKELLNIVRLPEGTTDKWFAKAGVDVWEDMPADTLAKCIEFVKNRL
                     PSALSAA"
BASE COUNT          908 a         1493 c         1624 g          971 t
ORIGIN      
        1 tgtttcgagc acaggcgcca cgccgggttc ggaggtactc cgccgacccg gattcttctg
       61 cgcgcgccgc agaatccgag tcgtcgcgag aacgcttcga acccggcgtg ggggcattca
      121 cattaaacac cagagacgcg cgcctcgact tctggcgggc gctttgcctg atcgacatgg
      181 tgctcgtgca cctggtgtac gagggcgtga atttcgggcc gctccagcgt gcgcttggcg
      241 aatacacgcg cttcgccgcc gggggatttg tcttcgtcgc gggactcagc atcggtgcca
      301 tcttcctgcc gcgcacgttc cggcccaatg ggcggcgcac cgtttacttt tcactgtggc
      361 gccgcgcgtt ttacatcctg tgcgttcatt atgcggcgac gttgagcttc gtgtcgctcg
      421 atttgatccg cgggatgcgc gctgaggtgc cgcacctgcc gccactgatg cgagacatcc
      481 tgctgctgcg cgaaggcggg gacctgctgc cgctatacgt gatcctcgtc gccgcgacgc
      541 cggtggtgct cgacctgctg cgccggccgc gctggcgctg gctgccggcg gtggcgagcg
      601 ttgggctgtt cgcctggggc cacgcgtggg aaccggcgct gcggtttccg atgggtcaga
      661 actttccggt cgtgctgtgg cagctgatgt tcaccggcgg cttgctgttc ggcgctgcgc
      721 tgccgcggta caacgcgctg gcaacgcgga cgaagacgat gctggcggcg ctggcgtgcg
      781 tcatctcagt cgtgctgtgg gcggcggatt accgaagcga tttcggctgg gcgtatccgc
      841 gcttgccgct ggtgttctgc aagacgccgt tgtcggcggg cgaagcgctg cgatatttcg
      901 ggctgacctt cacaatcatc ttttcgacgg acctgctgtg gaatcgcttg aacggcaacc
      961 gggttgtgaa atgggtgtcg cccatcggcc gaaagagcct gggctgctat gtcggacatg
     1021 catggatcgt gggagtcgtg gggatgatcg cgcatgcgac gaccggcgcg ggcgcgtggc
     1081 agatgatgct ggcgattccc gccgtcgtcg ccgtctggtt ggtggcaatt gcgctggaat
     1141 ggaatagttc tgtcacaaag cggatcgcgc ctccgacgcc cactctttcg cgttggctcc
     1201 cgattccctt ggcatgcagc gccgctctgg ttctgttctg cggcgttggt gtcatcgccg
     1261 cccggcgcat gccaccgccg gctgctgagt cgcaggccat cgcgattccc gacatcactg
     1321 acgacgacct cgacaccaat tcgaacaacg gtgcaccccc gtcgttcgac gccgcggatg
     1381 ccgcggatat cattccgacg tgacgatgca ggttcatcac ggtatcgacg gtttaaaatc
     1441 cctcccgccg gggtgtgtcc tgtcggtcgg caacttcgac ggcgtccatc gcgggcatcg
     1501 gcggcttctg gagatcgcca acgacctgcg cggcggtagt ggcgcgggcg gcgtcgccct
     1561 tgtcacgttt gaaccgcatc cgctcacggt gctgcggccg cagctggcgc ctccgcggct
     1621 cacgccgctg ccgttgaagc agtcgctgct ggaaccactc ggtgtcgatc acctgatcat
     1681 ccttcccccc acgccggacg tgctgaacct gacggccgaa gttttctggc agatcctccg
     1741 cgacgacgtg cggccgacgc acatgatcga aggtgagtcg ttcaacttcg gcaagggccg
     1801 gggtgggtcg atcaaccgcc tgcgcgaatg gtcggctaat actgacgtgc aactgcacgt
     1861 gattggggcg gtgcgcgtgc cgctgctgga cctgcaggtg gtggcggtga acagttcact
     1921 gatccgctgg ctgctgatgc acggccgagc ccgcgacgcg gcgatctgcc tggggcggcc
     1981 atacatcatc gagggcaccg tcattcaagg ccaccagcgc ggccggacga tcggggtgcc
     2041 gacggcgaac ctggactgtg gtgatcaact cgtcccggct gaaggtgtct acgtcgggcg
     2101 gtgccagatc gatggcacaa cccacgccgc ggctgcgagc atcgggacga tgcctacgtt
     2161 cggcgaaaac cagctgcagg ttgaagtgca cgtgatcgac ttcagcggcg acctctacgg
     2221 tcagacgctg cgcgtcgaac ttgtcgattg ggtccgcgag caatggcgat ttgcggatat
     2281 cgaggcgctg aagcagcgca tggcggggga tatcaaatgg tcgcgccggc ggttccacgc
     2341 gccggtcagc gagccttttg cttctttatc ctgagcgagg cacggactcg tgcgcagtca
     2401 aaggatctgg ctggcgtgtc ggagaataat cggttgcgaa gacggcaccg cggtcgcttc
     2461 caatttcgca gaccggtggc cgcggcttgt ccgtctgcga agccgcaagc gactgccacg
     2521 ccgatctcac caatcatgct tcaattatcc tcggccaaat ccttcggctt cgcacgagta
     2581 cgtgctgcgc tcagaattga gtaaattaac gcacgttcgc gccgtcgccg catctggcaa
     2641 taatctcaca gtcgatgtga ccaaacggcc gctatgcccg gacgcgacat catcatcatg
     2701 ggagccagca tcggggggat gcaggcgttt cagcgcattt gcgccgcgct gccggcggat
     2761 ctgccggcgg cggtgttcat cgtctggcat accgcgttgc attcaccggg catcttgccg
     2821 gatgtgctga atcgatcggc gcgcctgccg gtggcgttcg cgcgcgatgg ggaagcgatc
     2881 gagcatggtc gggtttacgt cgcgccgccg gatcatcaca tgctgctttg gcgcgatgtc
     2941 gtccagctga accgcgggcc gcggcagaat cggtttcgcc cggcagtcga tccgttgttt
     3001 cacagcgcgg ccgacgcgtt cggcccgcgc gtcgtcggcg tcatcatgac cggcgggttg
     3061 gacgatggca ccgcggggct gatgcacgtg aagcgcgaaa ggggcgtggc cgttgtgcaa
     3121 gacccgctgg acgcggacga accaggcatg ccgcgaagcg cgctggaaat ggtcgatgcc
     3181 gatttcgtcc tgccgatcgc gcaaatcccg gaggtgctgg tccgactggc gacggagcaa
     3241 ccgcagcctc cgattcgtct cgaggaggcg aaggcgcaga tgtacaacag cgacaaggac
     3301 atcggccaag gactccgccc cgaaggtgtg accggtccac ccgcggaggt cacctgcccc
     3361 gactgcagcg gcgccctttg gcgcgagacc gaggacggcg tcgtgaagta ccgttgccac
     3421 gtcggtcaca gcttcaccga gcagggcctg ctggcggcga agaatgaaga ggtggagctg
     3481 gcgctgtgga cggccctgcg cgtgctgaaa gaaaacgcga caatgtatcg caacatcatc
     3541 gagcgctcga cggccgcggg gcggctggca tcggcggagc atttcaagca gtcgctggaa
     3601 cagacggaat acgaactgca ggttattaag aaagcgctca tgacggactc gccgggaagg
     3661 gcgattcgcg gcgagcgaaa aagcgcgccg gattgagggg tggtcgtcgc ttgatgaacg
     3721 ccagctgtta aagcgcatct gcgcctttcc ccctctccca cgagtacgtg agagagcaga
     3781 ccagagtctt gcccggagtc gcagataacc gcctctcttt ttcgggcgtt tcacctgcca
     3841 tttttccctg aactgccgcc gctcagtaag cgtacaacct ctatgttcat cagagagtta
     3901 cgttgatcaa accttcttca aatcccgtcg catttaaaat aaattagacc ttgttttgcg
     3961 gcttctgttc gggtatccta acaattacct aaaagcggcg attcgcgtct ttagccggtg
     4021 ggtcgcgtcg ccagtcaaaa tcaagcatcg gcggcgttac agaacggagt ctcgtcatgg
     4081 cccttcgtgc caaaaaaccg gaagccgtca tcaagcggct caagctcttc atgtacggtc
     4141 ccgcgggcgt cggtaagacg acggcggcga tccagtttcc caactcttac atcatcgacg
     4201 ccgagcgcgg cgccgagaat tacgacaagc tggtgaccgc cagcgggtcg gcggtcttcc
     4261 agaccaacga catcaacgag gtcatctcgg aagtcaaagc gctgctcacc gagcggcacg
     4321 attacaagac gctggtgatc gatccgatca cgacgatcta caacgacctc ttggaaaagt
     4381 gcgagctgaa agtcggcacc gattttggcc ggcactatgg cgaggcgaac aagacgatga
     4441 agcgcctggc caacctgatt atggcgctgg acatgaacgt cgtcgtgacc gcgcatgcca
     4501 agaccgagta cggctcgaac ctttcgaagc tcggttacac cttcgacggc tggcggcagc
     4561 tggattactg gttcgacctg gttgtcgagc tgggcaagaa gaacaagaag cggttggcca
     4621 aggtcgtgaa gacgcgcgtc gagagcttcc ccgacgacga catcttcgag tggagctatg
     4681 aggcgatcca gaagcgctat gacatctcgg tgctggaaaa gcaggccgag caggtgcagc
     4741 tggccacgcc ggagcaggtg cgcgagatga aggaactgct gaacatcgtc cgactgcccg
     4801 agggcacgac ggacaaatgg ttcgccaagg ccggcgtgga cgtgtgggaa gatatgccgg
     4861 ccgacacgct ggccaagtgc attgagtttg tgaagaaccg gctgccgagc gcgctaagcg
     4921 ctgcttagcc gtgatgaatg attacgtgaa tacgtgagaa gaaggagtcc aatcacgtgt
     4981 tcacctcacc cgacgt
//
LOCUS       DATIMH010001093         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3434978, whole genome shotgun sequence.
ACCESSION   DATIMH010001093 DATIMH010000000
VERSION     DATIMH010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3434978"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VLI90_18870"
     CDS             <1..1071
                     /locus_tag="VLI90_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16334.1"
                     /translation="KWNAATGKGYWRQPPRDVAAFARFMSAAVARYRGVVHSWELWNE
                     PDNADYWAGSVEQYAQLVRAGAAAVRSADPSAKIVLGGISWNVAFLRQLFRDQHIGGA
                     IDIVNLHSYYETWIPDPLERLPEYVGRVHDLIARYGQGQTIWAAEIGYSDYRRGRSVS
                     ADNEARFEYEHTPAYQADDLFRALTLLRAGGEVSLATWYRINDLPVTQDVIGDVNNRH
                     LGVVTTTGQAKPALVALQEFQHLLGGPLRVIDNQSWVRRLAGSDAEVHCFGRADGDVI
                     AVLWLRTEVAGSEQSSTSGGDPRQEQVQVTVPMPFARASQCDLLDPAWQPLPIDATDG
                     LTTVSITHLHGGRTRVVRFSQR"
     gene            1281..1934
                     /locus_tag="VLI90_18875"
     CDS             1281..1934
                     /locus_tag="VLI90_18875"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSV16335.1"
                     /translation="MIINIAVAGGRKLLREGLSLLLDQQPDLKVIAESADVSTVPKLV
                     RALPVHVAVLNDSVGTNVAAEQVRAVLRANDAVHVVVLAMNPPVQWVRNVLDAGATAC
                     LTKECEGAELVSAIRMAHEGQLYLSPGLVHSLVTSQVQHKSKNAVFKSLAPREREILR
                     RIAEGQVTKEIAAELGVGVKTVETHRRRIMAKLNAFTVAELTKHAIRQGLTTLETPA"
     gene            complement(1996..3711)
                     /gene="ilvD"
                     /locus_tag="VLI90_18880"
     CDS             complement(1996..3711)
                     /gene="ilvD"
                     /locus_tag="VLI90_18880"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSV16336.1"
                     /translation="MATATETQTTPRLNKFSSRITQPASQGASQAMLIATGLSWKDLD
                     KPQIGIGSVWYEGNPCNMHLADLGNHVKRGAEDAGCVGMRFNTIGVSDGISMGTDGMS
                     YSLQSRDLIADSIETIMAAQWYDALVALPGCDKNMPGTVMAMGRLNRPSIMVYGGTIR
                     AGCATIRGVEEKLDVVSAFQAYGQRLANKITEDERKLLLSKACPGQGACGGMYTANTM
                     ASFIECMGLSLPYSSCAPADSQEKIDECVRAGSAIRHLLELDLKPRDIVTRQSFINAM
                     RLTIVLGGSTNAVLHSIAMARAFGIDLTIDDWQGVSDETPLLSDFKPSGKYVMEDLHA
                     VGGVPAVMKLLLAKGMLDGSQMTVTGKTIAENLRDLPGLSEGQQIVHSFDNPIKRDGH
                     IRILRGNLAPTGAVAKITGKEGTRFEGIARVFDAEEEMLHGLEAGRIGKGDVIIIRYE
                     GPKGGPGMPEMLTPTSALAGYGLLNDVALITDGRFSGGSHGFIIGHVTPEAIEGGNIA
                     FARDGDRVVIDAVKNTIDLLISEAELSSRRAAWKRPAYKATRGTLHKYIKNVRSASEG
                     CVTDE"
BASE COUNT          670 a         1171 c         1282 g          723 t
ORIGIN      
        1 aagtggaacg cggcgacggg gaagggttat tggcggcaac cgccgcggga cgtggcggcg
       61 ttcgcgcggt tcatgtcggc ggcggtggcg cgatatcgtg gcgtcgtcca ctcgtgggaa
      121 ctgtggaacg agccggacaa cgcagactat tgggcgggat cggtcgagca atacgcgcag
      181 ctggtgcgag ccggcgcggc ggcggtgcgc tcggccgatc catctgcgaa gatcgtgctg
      241 ggcggaatct cgtggaacgt cgcgttcctg cggcaactgt tccgcgacca gcacatcggc
      301 ggcgcgatcg acatcgtcaa cctgcacagt tactacgaga cgtggattcc cgatccactt
      361 gagcgattgc cggaatatgt cggccgagtg cacgacctga tcgcgcgcta tggccaaggc
      421 cagacgatct gggcggcgga gattggttac agcgactatc gccgggggcg aagcgtgtcg
      481 gcggacaatg aagcgcgctt cgagtacgaa cacacgccgg cgtatcaggc ggatgacctg
      541 tttcgcgcgc tgacgctgct gcgcgcgggc ggcgaggttt cgctggcgac gtggtatcgc
      601 atcaacgatc tgccggtgac acaggatgtg atcggcgacg tgaacaaccg gcacctgggg
      661 gtcgtcacaa cgacggggca agcgaaaccc gcactggtcg cgctgcagga attccagcat
      721 ctgctcggcg gcccgctgcg ggtcatcgat aatcaaagct gggtccgccg gctggccggg
      781 tcggatgcgg aagtccattg cttcgggcgc gcggacggcg acgtcatcgc ggtgctgtgg
      841 ctaaggacgg aagtcgcggg gtcggagcaa tcgagcacca gcggtggcga tccgcgccag
      901 gagcaagttc aagtgaccgt gccgatgcca ttcgcccgcg cgtcgcagtg cgacctgcta
      961 gacccggcgt ggcagccgct gccgatcgac gcaaccgatg gtctaaccac cgtctcgatc
     1021 acccacctcc acggcggccg cacgcgcgtc gtgcgcttca gccagcgatg aagcgaacgc
     1081 atctgaggtt tttcaaaatt gcggttttgc gcgctgactg attcggactc aacggtttaa
     1141 tttgctcatc acctcgcgag tacagggaag ttgtgtcgat ctgccgatga tcacctcagc
     1201 gggcacggaa gtttggaaat tcagaagcgg agaccgtgca cctaaggccg ccgttgcggc
     1261 aaataattgt ggatcgaaaa gtgatcatca acatcgcggt ggccggcggg cgcaagctgt
     1321 tgcgcgaggg gctgtcgctg ttgctcgacc agcaaccgga tttgaaggtg atcgccgaat
     1381 ccgccgacgt ctctacggtg ccgaagctgg tgcgggcgct gccggtgcac gtggcggtgt
     1441 tgaacgactc ggtgggcacg aacgtggccg ccgagcaggt tcgggcggtg cttcgcgcga
     1501 acgacgccgt tcacgtcgtc gtgctggcga tgaacccgcc ggtgcagtgg gtgcggaacg
     1561 ttctggacgc cggggcgacg gcgtgtctga cgaaggaatg cgaaggggcg gagttggtgt
     1621 cggccattcg catggctcat gaggggcagc tgtacctcag tcccggtttg gtgcactcgc
     1681 tggtgacgtc gcaggtgcag cacaaatcga agaacgcggt tttcaaaagt ttggcgccgc
     1741 gcgagcgcga gatcttgcgc cgcattgccg aaggccaggt cacgaaagaa atcgcggccg
     1801 agttgggcgt cggcgtcaag accgtcgaaa cccaccgccg gcggatcatg gcgaagctga
     1861 acgcattcac cgtggccgag ctgacaaagc acgcgatccg acagggcctg actaccctcg
     1921 aaacaccagc ttaatcggcg gcggcggcgt cgctgttcgg gcgtcccgct acgtgacggg
     1981 aacgcgctcg cgtcttcatt cgtcggttac gcatccttcg ctggcgcttc tgacgttctt
     2041 gatgtacttg tgcagcgtgc cgcgggtcgc cttgtacgcc gggcgcttcc atgcggctcg
     2101 gcgcgacgag agttccgcct ctgagatcag cagatcgatc gtgttcttca ccgcgtcgat
     2161 gacgacgcgg tcgccgtcgc gggcgaacgc aatattgccg ccctcgatcg cctccggcgt
     2221 cacgtggccg atgatgaacc cgtggctgcc gccggagaat cttccatcgg tgatcagcgc
     2281 gacgtcgttg agtaaaccgt agcctgcgag ggcgctggtc ggcgtcagca tctccggcat
     2341 gccggggcca cccttggggc cttcgtagcg gatgatgatg acgtcgccct tgccgattcg
     2401 gcccgcttcc aggccgtgca gcatttcctc ttcagcgtcg aacacgcggg cgatgccttc
     2461 gaagcgcgtg ccttccttgc cggtgatctt cgcgaccgcg ccggtcgggg cgaggttgcc
     2521 gcgcaggatg cggatgtggc cgtcgcgctt gatcgggttg tcgaacgaat gcacgatctg
     2581 ctgaccttcg ctgagcccgg gcagatcgcg aagattttcc gcgatcgtct ttcccgtgac
     2641 cgtcatctgc gatccatcga gcataccctt cgccagcagc agcttcatca ccgccggcac
     2701 cccgccgacg gcgtgcaggt cttccatcac gtacttgccg ctgggtttga agtcgctgag
     2761 caggggcgtc tcgtcgctga cgccctgcca gtcgtcgatc gtcagatcga tgccaaacgc
     2821 gcgggccatc gcgatcgaat gcagcaccgc gttggtggaa ccgccgagca cgatcgtcaa
     2881 gcgcatcgcg ttgatgaagc tctgccgggt aacgatgtcg cgcggcttga gatccagctc
     2941 cagcagatgc ctaatcgcgc tgccggcgcg gacgcattcg tcgatcttct cctgcgaatc
     3001 cgccggcgcg cacgatgaat acggcagcga caggcccatg cactcgatga acgacgccat
     3061 cgtgttggcc gtgtacatcc cgccacacgc gccttggccg gggcaggctt tgctgaggag
     3121 aagcttccgc tcgtcttcgg tgatcttgtt cgccaagcgc tggccgtagg cctggaacgc
     3181 cgagacgacg tccagctttt cctcaacccc gcggatcgtt gcgcagccgg cgcgaatggt
     3241 gccgccgtac accatgatcg acgggcgatt caatcgcccc atggccatca cggtgcccgg
     3301 catgttcttg tcgcaaccgg gcagcgccac cagcgcatcg taccactggg cggccatgat
     3361 cgtttcgatc gaatcggcga tgagatcgcg cgactgcagc gaatagctca tgccgtcggt
     3421 gcccatcgag atgccgtcgc tcacgccgat ggtgttgaag cgcatgccga cgcacccggc
     3481 gtcctcggcg ccgcgcttca cgtgattgcc caggtcggcc aagtgcatgt tgcacgggtt
     3541 gccctcgtac caaacgcttc cgatgccgat ctgcggcttg tccaggtcct tccaggacaa
     3601 accggtggcg atcagcatcg cttgcgaagc gccctggctg gcgggctgcg tgatgcgcga
     3661 cgagaacttg ttcaaccgtg gagtcgtttg tgtttcggta gcggtggcca tgatcgaaat
     3721 agtatgaggg gaagagcgga atggaaaccc gctcgacgcc ggtgtctgag gtactccgaa
     3781 gactcggatc cttcgcgcta acaatccggg tcttcggagt acctcagaca ccggcgtcga
     3841 gggcgc
//
LOCUS       DATIMH010001094         4657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5463120, whole genome shotgun sequence.
ACCESSION   DATIMH010001094 DATIMH010000000
VERSION     DATIMH010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5463120"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1226)
                     /locus_tag="VLI90_18885"
     CDS             complement(75..1226)
                     /locus_tag="VLI90_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16337.1"
                     /translation="MKIVVGGYIVGFPLGGMTWHHLNYLLGLHELGHEVYFLEDSGRY
                     SIPYNPTTWSCSVDSTYGRAYLERTFQKYGLPLRYCYYSEFEDQHYGMSREELADVLK
                     RADLLIAVSGVTPLRDERPRPRRTAVIDTDPVFTQLRMRHDANFLNYYRQFDAVATFG
                     RLIGTDACPLPTHGFDWIPTNQPIALRHWPVMPVTSRRFTTVGKWEHTSDRNLEFAGR
                     TFHSSKGVEWTKVIDLPRRTRWEMAIGMAAITPAAKEQFTQSGWQLLDAEAATISCEA
                     FQQFIQNSAGEFTVAKEIYAGLPSGWFSDRSAAYLTSGRPVVTQASGFDQWLPVGEGL
                     FSFETIDQAAWALEQIERDYAMQSAAARRMAERNFDSRLVLSKLLDRAL"
     gene            complement(1416..1700)
                     /locus_tag="VLI90_18890"
     CDS             complement(1416..1700)
                     /locus_tag="VLI90_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16338.1"
                     /translation="MFGWLTQHLLKRATNRSANLIFDKVSVHLTADGFELQEAERMLA
                     QVKWLDIVEARAFKRDLLTTDRICVLCRIGTRAPNDFGVEVHEEMPGFID"
     gene            complement(1710..2885)
                     /gene="sucC"
                     /locus_tag="VLI90_18895"
     CDS             complement(1710..2885)
                     /gene="sucC"
                     /locus_tag="VLI90_18895"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HSV16339.1"
                     /translation="MKIHEYQARQILAEAGIPVPPAEVVRTPDEAAAALKMFAQPMCV
                     VKAQVYAGGRGKAGFVKLVKSADEARDAAKFMLSHRMVSVQTGPEGVPVSVLIVAPGV
                     DIAKEYYIGITVDRGRNTATMIASAEGGVEIEEVARKTPEKVLKEPLHPLMGLQGYQA
                     RQLAFKLGFRDKLAGEAAKLMINLAKVFLKTDASLAEINPLVITKDGKLLAIDAKINF
                     DDNALFRHNDIVAMEDESQEKPLDVRAKKASLNYIALDGTIGCLVNGAGLAMATMDII
                     KLYGAEPANFLDVGGSVSAEGAIEAFRIILADPKVKGILVNIFGGIAKCDLIAEALVK
                     AGQEVGFRVPVVVRLEGTNADKAREILAAARSQLPTLTPASDLTDAAKKVTAAVKAA"
     gene            complement(2905..3153)
                     /locus_tag="VLI90_18900"
     CDS             complement(2905..3153)
                     /locus_tag="VLI90_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16340.1"
                     /translation="MIDLPYSMVIEATADPEFFGFYSTELQGFTGIGHSVEDCLYKAR
                     WGMKDHVKLLRQRRLPVPASTANPTIIIQNQAKRRKSA"
     gene            complement(3150..3344)
                     /locus_tag="VLI90_18905"
     CDS             complement(3150..3344)
                     /locus_tag="VLI90_18905"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HSV16341.1"
                     /translation="MRFSELVRLLEKNGFSLAKEKGSVRYYAKSGCPNLVRVDFHGAK
                     EVPTGTCHAILKAAGLKGKQ"
     gene            3412..4119
                     /gene="queC"
                     /locus_tag="VLI90_18910"
     CDS             3412..4119
                     /gene="queC"
                     /locus_tag="VLI90_18910"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HSV16342.1"
                     /translation="MESERHSAIVLTSGGLDSTTCLAIAKADRFAKLYSLTFDYGQRH
                     RQELDAAARVASEFGAAEHRVIHVDLRQFGHSALTDDIAVPKDRTESAMSARIPVTYV
                     PARNTIFLSYALAWSEVLGVPDVYIGVNAIDYSGYPDCRPTFIEAFERMANLATEMTT
                     RGGEDRRFKIHAPLIQMSKSQIILRGVALGVDYSMTHSCYDPSPEGLACRRCDSCLLR
                     KKGFAEAGVADPTKYVE"
     gene            4192..4569
                     /locus_tag="VLI90_18915"
     CDS             4192..4569
                     /locus_tag="VLI90_18915"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HSV16343.1"
                     /translation="MKAVSIKQPWASLIAAGVKTLELRAWPTDHRGPLLICSSRRPFI
                     EGHRHGAALCVVNVVDCRAMTPDDVPFACVDQFYANHYAWVLRDVRLVEPFSIVGQLR
                     LFDVRDNLIHPTKLESATTGRPA"
BASE COUNT          863 a         1491 c         1360 g          943 t
ORIGIN      
        1 ggatgatttg acgatcgtca tccttgaagt gcgctgaata acaggcgaaa gctcacggct
       61 ctgccgtggg accattacag tgcgcggtcc agcaatttcg agagcaccaa ccgcgaatcg
      121 aagttccgct ctgccatgcg ccgcgccgca gcgctctgca tagcgtaatc ccgctcgatc
      181 tgctccaacg cccacgccgc ctgatcgatc gtctcgaacg aaaacaatcc ctcccccacc
      241 ggcagccatt gatcgaagcc gctcgcttgc gtgaccaccg gccggccgct ggtcaggtat
      301 gcggcgctgc ggtcactgaa ccacccgctg ggcaggccgg cgtaaatctc cttcgcgacc
      361 gtgaactctc cagctgagtt ctggatgaac tgctgaaagg cctcacagct gatcgtcgcc
      421 gcctcggcgt cgagcaattg ccatccactc tgcgtaaatt gctccttcgc cgccggcgtg
      481 atcgcggcca taccgatcgc catctcccag cgcgttcgcc ggggcaggtc gatcaccttc
      541 gtccactcca cgcccttgct gctgtggaac gtgcgcccgg caaattcgag attgcgatcg
      601 ctcgtgtgct cccacttgcc gacggtggta aaacgccggc tcgtcaccgg catcaccggc
      661 caatgccgca gggcgatcgg ctggttcgtc gggatccaat cgaacccgtg cgtcggcagc
      721 ggacaggcgt cggtcccgat caatcgcccg aacgtcgcaa cggcgtcgaa ctgccgatag
      781 tagttgagaa aattcgcatc gtgccgcatg cgcagctgcg tgaacacggg atcggtgtcg
      841 atgacggcgg tccgccgcgg ccgtgggcgc tcgtcgcgca gcggcgtgac accgctgaca
      901 gcgattagaa gatcggcgcg cttcagcaca tccgccaatt cctcgcggga catgccgtag
      961 tgctggtctt caaactccga atagtagcag taccgcagcg gcagaccgta cttctggaac
     1021 gtgcgctcga gatacgcccg gccgtaggtg ctgtcgacgc tgcagctcca cgtcgtgggg
     1081 ttgtagggga tggagtatcg cccactgtct tcgagaaaat agacttcgtg cccgagctcg
     1141 tgaagcccga ggaggtagtt gagatgatgc cacgtcatcc cgccgagcgg gaagccgacg
     1201 atgtaaccgc caaccacgat cttcaaggga ggtgacatct gtatgtagca cgggcatctc
     1261 gcccgtgccg cgcggttctc gacacgggcg agacgcccgt gctacttaat tgtcgcgttt
     1321 ccagatcacc cgctcgcacg ttttcatcgg cggcagcatg accgatccat accattcggc
     1381 gtcaaggccg ggcagatgcg cgggcagcgc attcatcaat cgataaagcc cggcatctct
     1441 tcatgcactt cgaccccgaa gtcgttgggc gctctcgtac caattcgaca caaaacacag
     1501 attcgatccg tggtgagaag atcgcgctta aacgccctgg cttctacaat atccagccac
     1561 ttcacctgag caagcatccg ctccgcttct tgaagctcaa acccgtcagc ggtgagatgg
     1621 accgagactt tgtcgaaaat caggttggcg gaacggttcg tcgctcgctt gagaagatgc
     1681 tgcgtcaacc agccgaacat aacgcgcctt tacgctgcct tcacggcggc ggtcactttc
     1741 ttcgcggcgt cagtcaaatc cgacgccggc gtcagtgttg gcaactgcga cctcgccgcc
     1801 gcgagaatct cgcgagcttt atccgcgttc gtgccttcca ggcgcacgac gaccggcacc
     1861 ctgaagccca cttcctgccc cgccttcacc agcgcttcgg cgatcaagtc acatttcgcg
     1921 attccgccga agatgttgac gaggattcct ttgactttcg gatcggccag gatgatgcgg
     1981 aacgcttcga tcgcgccttc ggcgctgacg ctgccgccga cgtccaagaa gtttgccggc
     2041 tcggcgccgt acagcttgat gatgtccatt gtcgccatcg ccagccccgc gccgttgacc
     2101 aggcagccga tcgtgccgtc gagcgcgatg tagttcaggc tcgccttctt cgcccgcacg
     2161 tccagcggct tctcctggct ctcgtcttcc atcgcgacga tgtcgttgtg gcggaacagc
     2221 gcgttgtcgt cgaagttgat cttcgcgtcg atcgccagca gtttgccatc cttggtgatg
     2281 accagcggat tgatctccgc cagcgacgcg tcggtcttca gaaacacttt ggcgaggttg
     2341 atcatcagct tggccgcttc gccggccagc ttgtcgcgaa aacccagctt gaacgccagc
     2401 tggcgcgcct gatatccctg caatcccatc agcggatgca gcggttcctt cagcactttc
     2461 tccggcgtct tgcgcgcaac ctcttcgatc tccacgccgc cctcggccga cgcgatcatc
     2521 gtcgcggtgt tgcgcccgcg atcgacggtg atgccgatgt aatattcttt ggcgatatcg
     2581 acgcccggcg cgacaatcag caccgacacc ggcacgcctt cgggcccagt ctgcacgctg
     2641 accatgcggt ggctcagcat gaacttggcc gcgtcgcgcg cctcatcggc ggacttcacc
     2701 agcttgacga accccgcctt cccgcgtccg ccggcgtata cctgcgcctt cacgacgcac
     2761 atgggttgag cgaacatttt caacgccgcc gccgcctcat cgggcgtacg cacaacttcc
     2821 gcgggcggaa cgggaatgcc ggcttcggca aggatctggc gggcttggta ttcgtggatt
     2881 ttcattgtcg ttgcgtgtcc gtcgttatgc cgatttccgg cgctttgcct ggttctgaat
     2941 gatgatggtt ggattcgccg tcgatgccgg cacgggcagg cggcgctgac gaagcaattt
     3001 cacgtggtcc ttcattcccc accgggcctt gtagaggcag tcttcgaccg agtgaccgat
     3061 gccggtgaat ccttggagtt ccgtggaata gaagccaaag aattccggat ccgcagtcgc
     3121 ttcaatcacc atcgagtaag gtaaatcgat cattgcttac ctttcagccc ggccgccttc
     3181 agtatcgcat gacaggtgcc ggttggaact tccttggcac cgtgaaagtc aactcgcact
     3241 aagttcgggc agccactctt ggcgtaatag cggaccgagc ccttttcctt ggccagcgaa
     3301 aagccgttct tctccagcag tcgaacgagt tcgctgaatc tcatccagtt gcattttagc
     3361 agcaactccc atcgcctgac agcttcgcgc gcgaacggta agattcgccc gatggaaagc
     3421 gaacgccatt ccgcgatcgt cctcaccagc ggtgggctcg acagcacgac ttgcctggcg
     3481 atcgccaagg ccgatcgctt cgccaagttg tattcgctga cgttcgacta cggccagcgg
     3541 catcgccagg agctggacgc ggcggcgcgc gtcgccagcg agttcggggc ggccgaacat
     3601 cgcgtgatcc acgtcgacct gcgccagttc ggccacagcg ccctgaccga tgacatcgcg
     3661 gtgccgaagg accgcacgga atcggcgatg agcgcgagaa tcccggtcac ctatgtgccc
     3721 gcgcgcaaca cgatcttcct cagctacgcc ttggcctggt cggaagtgct gggtgtgccg
     3781 gatgtctaca tcggcgtcaa cgcgatcgat tacagcgggt atcccgattg ccggccgacg
     3841 ttcatcgaag cgttcgaacg catggcgaac ctggcgaccg agatgaccac ccgcggcggc
     3901 gaggatcggc ggttcaagat ccacgcgccg ctgattcaga tgagcaagtc gcagatcatc
     3961 ctccgtggcg tggcgctagg cgtcgattat tcgatgacgc acagttgtta cgacccgtcg
     4021 cccgagggcc tggcctgcag gcgttgcgac agctgcctgc tgcggaagaa ggggtttgcc
     4081 gaagcgggcg tggcggatcc gacgaagtac gtggagtgaa gcgtgaatac gtgagcgtag
     4141 cacgatatcc ttctcacgta ataacgtaat cacctcacac gggcattctt gatgaaggct
     4201 gtctcgatca aacaaccctg ggcgtcgctg atcgcggcgg gcgtgaagac gctggaactg
     4261 cgggcgtggc ccaccgacca ccgcgggccg ctgttgatct gttcgagccg tcggccgttc
     4321 atcgaggggc atcggcacgg cgcggcgtta tgcgtggtga acgtggtgga ttgccgcgcg
     4381 atgacgccgg acgatgtgcc gttcgcctgc gtcgatcaat tttacgccaa tcattatgcg
     4441 tgggtgctgc gcgatgtgcg gctcgtggag ccgttctcaa tcgtcggaca attgcggctc
     4501 ttcgacgtgc gcgacaacct gatccaccca acaaaactcg aaagcgccac cacaggccga
     4561 cccgcttaag cgggtcgggc cagtaggacc ggcgttccat ccggtccttc gattcggacc
     4621 atcttcacac catacacgcg cgagagcatc tccggtt
//
LOCUS       DATIMH010001095         6090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5463360, whole genome shotgun sequence.
ACCESSION   DATIMH010001095 DATIMH010000000
VERSION     DATIMH010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5463360"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VLI90_18920"
     CDS             <1..1143
                     /locus_tag="VLI90_18920"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HSV16344.1"
                     /translation="DAGVDPAGITSGVVGNFAAGLFTRQLHLGAMLTGVDPKLRGIPT
                     LHTEAACASGGVAVLTAAQQIMAGLHECVLVVGVEQQKTMSPTDGADVLGAAADYTEE
                     RARFGDFMFPKLFGRIAQLYAERYGLTERQLALVAVKNYAHARLNPCAQMRDRQLTLE
                     QAMTESAENARLAPPLKTCDCSQITDGSAALLLCSERVAARISKDRRVRLLGFGHTTD
                     HLPLDRKDAPLFSIARSSAAKAYAMAGLSPRDIDAADVHDCFSISEILATEIIGFAEP
                     GEGTGLIESGATMLPAVREQLGIPTPSRSIPINPGGGLIGDGHPVGATGVRQVVEAYQ
                     HLTRRAGARQVAGTKRYLTFNMGGTVTTNVTMIWGNDAGAEAQSAR"
     gene            complement(1246..3189)
                     /locus_tag="VLI90_18925"
     CDS             complement(1246..3189)
                     /locus_tag="VLI90_18925"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HSV16345.1"
                     /translation="MSAKHLITLIVSSTIAVLAATTQAVPYHGVFTEAAPTSIQPQGW
                     LAEILHRQVTGLATHHAASGYPYDTCLWTGKIGKGGDSPYWKPWWPYEQTGYLVDGLE
                     RLGLLTNDPQVSAEAAANIRYILDHPAADGSLGPDHIGPTNWPHAVVFRAIEAAYAAH
                     PDPAIPEALRRHYLSRPADYGIGRDVCNVESILWTYAHTGDERLLTLAQRTYDNFNHT
                     KAATTLPSLANARKIVEHGVTFNETAKLPALLYLYTGDRALLDATVNAYEKIDRDHML
                     ASGMHSAEERLDGQDAWGYTEVCDISDYTWSVGYLLMSTGDATWADHIERAVFNAGLG
                     AITKDFKAHQYFSSPNQVVATNGICKRYNPNRLAYRAGHDVQCCSGNVERFLPNYALR
                     QWMQNADGGVAAVMYGPSRFSTTIDGQAVTIDEQTDYPFSETITFVVHVAKPAAFGLS
                     LRLPGWTKNPSVAVNDQPLTNAEQPGQFTTIRRTFNDGDKIVLKLPMPIVIHHWPHDT
                     ASIERGPLVFSLKIGESATPVQDATMQPGFPAWDKRPASPWNCALAISDGGSEQLQLV
                     SKPTNGFPFDIGHSPIELQAPARVITNWKLTDNGGNPGFPTSPQLADEVERVTLVPYG
                     STCLRLTVFPTVAPTERTVGAMR"
     gene            complement(3301..5568)
                     /locus_tag="VLI90_18930"
     CDS             complement(3301..5568)
                     /locus_tag="VLI90_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16346.1"
                     /translation="MLSIARSVNGLFVSLIVLCAISAARAQTIRNDLGWTIELTPDHG
                     DYTIDAADPKWTVGGSLGKSATDVATADGADRIGPYHELHFKWREEGQRSGNIRLYNN
                     KPIVLFSVTFDEAVNHSPAPFPVLTRLPQDLLEFRYGDIDHLRPESFSLATSSKDAVP
                     DAKGEPQEPQYGGPFALFDAHATTLILSPASDFMVAQLSGDQKQGLRSGLNATLASVP
                     AGYSYETILAIGSGINRTWDTWGQALTDHYAKHRPANDADAGLKYLGYWTDNGGTYYY
                     HYDPTLGYAGTLLAVKQHLDQIGVPIGYMQIDSWWYPKTFNSVQAKASNKPRSKDPSI
                     PAGTWNRYGGLLEYTAHPDLFPHGLGSFQKQLGVPLITHNRWIDPESPYHQAYKISGI
                     AAVDPAYWDKIIGDIAGWGVSTYEQDWLNYIYLRSPELFSTTWAGDACMDAMAAACAR
                     NGLTMQYCMVLPRDLMQGGAKYPNLTTVRVSGDRLDRGKWREFIYGSRLPASLGVWPW
                     TDAFRSKETANLLIATLSAGMVGINDKMGDEDVANIFQSVRKDGVIVKPDVPMTPVDA
                     TFIAEAQGEHVGPLVCATQTTHGDAKAMYIFAFEPKAPAKGAEAKTFTFHARSSAVYA
                     YDYFNGTGRLIPNGELSEKLGPDGWSYWVLAPVGLSRMALIGDTGKFITRGKQRITAI
                     SDTSGRLSATLAFAPKEDAVTIAAYAPAQPSVQVTNGACRKLTYDSATKIARADIAPE
                     GEARGSVVDVQFMLP"
     gene            complement(5884..>6090)
                     /locus_tag="VLI90_18935"
     CDS             complement(5884..>6090)
                     /locus_tag="VLI90_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16347.1"
                     /translation="ERLVNRIQGRDERVHAVLPTHLVVRNSTAIVRQAHAQHEGPPPS
                     LASVPDGYSAPKLTPARDEPAPPS"
BASE COUNT         1008 a         1853 c         2058 g         1171 t
ORIGIN      
        1 gacgccggcg ttgatcccgc tggcattaca tccggcgtcg tcggcaactt cgccgcgggg
       61 ctgttcacgc ggcaactgca cctgggcgcg atgttgaccg gcgtggaccc caagctccgc
      121 ggcattccca cgctgcacac cgaagccgcg tgcgccagcg gcggcgtcgc ggtgctcacc
      181 gccgcccagc agatcatggc cggcctgcac gaatgcgtgc tggtcgtcgg ggtcgagcag
      241 cagaagacca tgtcgccgac ggatggcgcg gacgtgctcg gcgccgccgc ggattacacg
      301 gaggaacgcg cgcgcttcgg cgacttcatg ttcccaaaac tgttcggccg gatcgcgcag
      361 ctctacgccg agcgatatgg attgaccgaa cgccagctcg cgctggtggc ggtgaagaac
      421 tacgcgcacg cgcggctcaa tccatgcgcg cagatgcgcg accggcagtt gacgctcgag
      481 caggcgatga ccgaatcggc ggagaacgcg cgcctcgcgc cgccgctgaa gacctgcgac
      541 tgctcgcaga tcactgacgg ctcggcggcc ctgctgctgt gctccgagcg cgtcgccgcc
      601 cggatatcaa aggatcgccg cgtgcggctg ctcggcttcg gtcacacgac cgatcacctg
      661 ccgctggatc gcaaggacgc gccgttattt tcgatcgcgc gatcgtccgc cgccaaagcg
      721 tacgcgatgg ccggcctgtc gccgcgcgat atcgacgcgg cggacgtgca cgattgcttc
      781 tcgatctccg agatcctcgc gacggagatc atcggcttcg ccgagccggg cgagggcacg
      841 gggctgatcg aaagcggcgc gacgatgctg cccgcggtgc gcgagcagct cggcatcccc
      901 acaccctccc gatccattcc catcaacccc ggcggcggct tgatcggcga cggccatccc
      961 gtcggcgcca ccggtgtccg gcaagtcgtc gaggcgtatc agcatctcac ccggcgcgct
     1021 ggcgcgcggc aagtcgccgg cacaaagcgc tatctcacat tcaacatggg cggcacggtc
     1081 acgacaaacg tgacgatgat ctggggcaac gatgccggcg cagaagcaca aagcgcgcgt
     1141 tgatagccac aggcgacagc cagcgggggc gcgagacgtt gagcgacgct actttcgaaa
     1201 ttcccgctgg ctgtcgcctg cggctactaa agccgtcggt ggatcttatc gcatcgcgcc
     1261 gaccgtgcgc tctgtaggcg ccaccgtcgg aaacaccgtc aggcgaaggc atgttgagcc
     1321 atacggcacc agcgtcacgc gctctacttc gtccgccaac tgcgggctcg tcggaaagcc
     1381 ggggttcccg ccgttgtcgg tgagcttcca gttggtgatc acgcgagcgg gcgcttgcag
     1441 ctcgatcggc gagtggccga tgtcaaacgg aaatccgttc gtcggcttcg acaccagctg
     1501 cagttgctcg ctgccgccgt cgctgatcgc cagtgcgcaa ttccacggtg acgcggggcg
     1561 cttgtcccat gcggggaagc cgggttgcat cgtcgcgtcc tgcacgggcg tggcgctctc
     1621 gccaatcttc aatgaaaaga ccagcgggcc gcgctcgatg ctcgcggtgt cgtgcggcca
     1681 atggtggatg acgatcggca tcggcagctt caggacgatc ttgtcgccgt cgttgaacgt
     1741 ccggcggatc gtggtgaact ggcccggctg ctcggcgttc gtcagcggtt ggtcatttac
     1801 cgcaaccgac ggattcttcg tccaacccgg caggcgcagc gacaacccga acgcggctgg
     1861 cttggccacg tgaacgacga aggtgattgt ctcgctgaac ggataatccg tctgctcgtc
     1921 gatcgtcacc gcttgtccgt cgatcgttgt gctgaaccgg ctcggcccgt acatcaccgc
     1981 ggcgacgccg ccgtctgcgt tctgcatcca ctgccgcagc gcgtaattcg gcaggaagcg
     2041 ctcgacgtta ccggagcagc attgcacgtc atgaccagcg cgatacgcga ggcgattggg
     2101 gttgtagcgc ttgcagatgc cgttggtggc gacgacttga ttgggcgaag agaaatattg
     2161 atgcgctttg aagtcctttg tgatcgcgcc gagcccggcg ttgaagacgg cacgctcgat
     2221 gtgatccgcc cacgtcgcgt cgccggtgga catcagcagg taaccgacgc tccaggtgta
     2281 gtcggagatg tcgcagacct cggtgtaccc ccatgcgtcc tgcccgtcga ggcgttcctc
     2341 agccgagtgc ataccgctgg cgagcatgtg gtcgcggtcg atcttttcgt acgcgttgac
     2401 cgtcgcgtcg agcagcgcgc gatcgccggt gtacagatag agaagggcgg ggagttttgc
     2461 ggtttcgttg aacgtgacgc cgtgctcgac gatcttgcgc gcgttggcca gcgacggcag
     2521 cgtggtggcg gctttggtgt ggttgaagtt gtcgtaggtg cgctgcgcca gcgtcagcaa
     2581 ccgctcgtcg ccggtgtggg cgtaggtcca caggatcgac tcgacgttgc agacgtcgcg
     2641 gccgatgccg tagtcggcgg gacgcgacag gtaatgccgg cggagcgctt cgggaatggc
     2701 cgggtcggga tgggcggcgt aggcggcttc gatggcgcgg aagacgaccg cgtgtggcca
     2761 attggtcggg ccgatgtgat caggcccgag cgagccatcg gcggcggggt ggtcgaggat
     2821 gtagcggatg ttggcggcag cttcggcgct gacttgcggg tcgttggtca gcaggccgag
     2881 gcgctcgagg ccatcgacga gataaccggt ctgttcgtat ggccaccacg gtttccaata
     2941 cgggctgtcg ccgcctttgc cgatcttgcc ggtccagagg caagtgtcgt acggataccc
     3001 gctggcggcg tggtgcgtgg cgaggcctgt gacctggcgg tggaggattt ctgcgagcca
     3061 gccttggggt tggatggatg tgggggccgc ttcggtgaag acgccgtggt aggggacggc
     3121 ctgtgtggtt gctgcgagta ctgcgattgt agaagaaaca atcagggtga tcaggtgctt
     3181 tgctgacaat gagcgcgcgg atgcggcacg atgatgcctg tcacggagcc ccggatgtac
     3241 atccggggct tcgttttcat acttcagagc atcaacaaat tggtgcattc ttctctccca
     3301 ttatggcaac ataaattgca catcgacaac cgagccgcgc gcttcgccct ccggtgcgat
     3361 atcggctcgc gcgatcttcg ttgcgctatc gtacgtcagc ttgcggcagg ccccgttcgt
     3421 gacctgcacg ctcggttgcg ctggggcata cgccgcgatc gtcaccgcgt cttctttggg
     3481 cgcgaaggca agcgttgcgc ttaaacggcc cgacgtgtcg ctgatcgccg tgatgcgctg
     3541 cttcccgcgc gtaatgaact tccctgtgtc gccgatcagc gccatgcgcg ataggccgac
     3601 gggcgccagc acccagtacg accagccgtc ggggccgagc ttttcggaca gctcgccgtt
     3661 cggaatgagt cgaccggtgc cgttgaaata gtcgtacgcg taaacggccg atgatcgagc
     3721 gtggaacgtg aacgtcttgg cctccgcccc cttcgccggc gcttttggct cgaacgcgaa
     3781 gatgtacatc gccttcgcgt caccatgcgt cgtctgcgtc gcgcacacca atggcccgac
     3841 gtgttcgcct tgcgcttcgg cgatgaacgt cgcgtccacc ggcgtcattg ggacgtcggg
     3901 tttgacgatg acgccgtcct ttcgcacgga ctggaaaatg ttggcgacgt cttcatcgcc
     3961 catcttgtcg ttgatgccga ccatgccggc ggacaacgtg gcgatcagca ggttggcggt
     4021 ttccttgctg cggaaggcgt cggtccacgg ccagacgccg agcgacgccg gcaggcgcga
     4081 gccgtagatg aactcgcgcc acttgccgcg gtccaagcgg tcgccactga cgcgcaccgt
     4141 cgtcaggttc ggatatttag cgcccccttg catcaggtcg cgcggcagca ccatgcagta
     4201 ttgcatcgtg agcccgttgc gcgcgcacgc ggcggccatc gcgtccatgc acgcgtcgcc
     4261 cgcccaggtg gtgctgaaca gttccggcga gcgcaggtag atgtagttca gccaatcctg
     4321 ctcgtacgtg ctgacgcccc aaccggcgat gtcgccgatg atcttgtccc agtacgcggg
     4381 atcgaccgcg gcgatgccgc tgatcttgta agcctggtga tacggactct ccggatcgat
     4441 ccagcgatta tgcgtgatga gcggaacgcc gagttgtttc tggaacgagc caaggccgtg
     4501 cgggaagagg tcgggatgag cggtgtattc gagcaatccg ccatagcggt tccacgtgcc
     4561 ggccgggatg ctggggtctt tcgatcgcgg cttgttcgac gcctttgcct gcacgctgtt
     4621 gaacgttttt gggtaccacc agctgtcgat ctgcatgtaa ccgattggca cgccgatctg
     4681 gtcgaggtgc tgcttgacgg ccagcaacgt gccggcgtaa cccagcgtcg gatcgtaatg
     4741 gtagtagtac gtcccgccgt tgtcggtcca gtagccgaga tatttgagcc ccgcgtcggc
     4801 gtcgttagcg gggcgatgct tggcgtagtg atcggtcagc gcttggcccc aggtgtccca
     4861 cgtgcgattg atcccgctgc cgatcgcgag gattgtttca taactgtatc cagcggggac
     4921 ggacgcgagg gtcgcattca ggccgctgcg caggccttgc ttctgatcgc cgctgagctg
     4981 cgcgaccatg aagtctgacg cgggggagag gatgagcgtg gtcgcgtgcg cgtcgaacag
     5041 cgcgaacggc ccgccatact gcggctcctg cggttcgccc ttggcatcgg gcaccgcgtc
     5101 cttcgatgac gtcgcgaggc tgaacgattc ggggcgaagg tgatcgatgt cgccgtagcg
     5161 gaattcgagc aggtcctgcg gcaggcgggt gagcacggga aacggcgcgg gcgaatggtt
     5221 cacggcttcg tcgaacgtga cggagaagag cacgatgggc ttattgttgt agagccgaat
     5281 gtttccgctg cgctggcctt cttctcgcca cttgaagtgc agctcgtgat acggcccgat
     5341 gcgatcggcg ccgtcggcgg tggcgacgtc cgtagcggat ttgccaagcg aaccaccgac
     5401 ggtccatttg ggatcggcgg catcgatcgt gtagtcgccg tggtcgggcg tgagttcaat
     5461 cgtccaaccg agatcgttgc gaattgtctg ggcgcgagca gcgctgatgg cgcagaggac
     5521 gatcaagctg acaaacagcc catttactga gcgcgcgatt gataacatgc gtccgcgcct
     5581 cgtccccctc tcccacgtac tcgcgggaga gggccggggt gagggtgact gcgagcacca
     5641 gcggctttgg atgttggaaa tcaccctcac cctaaccctc tcccacgcgt acgtgggaga
     5701 ggggaccaga taaggagcgc gctttaacat gcctgcgatc agtagccccg cgtgaacacc
     5761 cgcggagttt acgtggtgcg ttgaggactt tcgatcgctc aaagagatga cccttgaagt
     5821 tccgagttaa cccggcgtgt gtcacgccgg gctaaatggg acggttgacg gtcgttgcgg
     5881 cctttacgaa ggcggcgcgg gctcgtcgcg cgcgggcgtg agcttcggcg cggaatatcc
     5941 atccggcacc gacgccagcg acggcggtgg cccttcgtgc tgcgcgtgcg cctggcggac
     6001 gatggcggtc gaattgcgaa cgaccaagtg cgtgggcagc accgcgtgca cgcgctcgtc
     6061 gcggccttga atgcggttga ccaaccgctc
//
LOCUS       DATIMH010001096         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5174758, whole genome shotgun sequence.
ACCESSION   DATIMH010001096 DATIMH010000000
VERSION     DATIMH010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5174758"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..469)
                     /locus_tag="VLI90_18940"
     CDS             complement(107..469)
                     /locus_tag="VLI90_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16348.1"
                     /translation="MIFNPLVIAVMAGVGLVAARALDVNPQMREMYIAAGVMLAASEL
                     AMLPLLLCRGANQLTMSQAALVATMIHLMSAAAAALALTASKPFTYWLLAFYGGTLIS
                     VSIACIRAVRSAPIATKQ"
     gene            complement(518..727)
                     /locus_tag="VLI90_18945"
     CDS             complement(518..727)
                     /locus_tag="VLI90_18945"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HSV16349.1"
                     /translation="MARDDDSDWGRNLALGMEVAVGVGIGYFIGHWADKRFGWAPWGV
                     YAGVMLGCAAGMYSLIKESVRSNKR"
     gene            complement(900..2513)
                     /locus_tag="VLI90_18950"
     CDS             complement(900..2513)
                     /locus_tag="VLI90_18950"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HSV16350.1"
                     /translation="MHLFILIVLIACSLAAAPASQPASVPLKVEQWGRFEVALPGPRE
                     GNPFLDVELSATFTWNEIARTVRGFYDGDGVYRIRFMPPAVGVWRFVTRSNRAELNGR
                     AGELTVTAPSPGNHGPVRVAGRYHFAYADGTAFVPIGTTCYAWVHQPEALEQRTLATL
                     KASPFNKVRMCVFPTAYKKGGDEPPRFPFERSADGAFDHARFNVAFFRHLEQRIAQLG
                     ELGVEADVILFHPYDKGTMGFDRMPAGDDDRYVHYLVARLSAYRNVWWSMANEFDLMH
                     EKHDVDFDRLFQILQADDPADHLRSIHFSARMYDSSKPWITHLSVQNGAAVDDFGRAS
                     LYRDVAGKPVIYDEARYEGNIERRWGQLSAEQMVEHCWLGTIGGTFVGHGEVLRDQSG
                     ESWTSRGGELRGQSAARIAFLKSILTEAPPVSPIDRFYETHVGGRAGEFYLVYFGSER
                     PAEWAFQLPRIGLEEGMTFRVDVLDTWNMTSTPVEGPAVRLVKQGMYAFAAEGERKIA
                     LPGRPWMALRIVRVGAAPTTAVAPVNEAE"
     gene            complement(2638..>4230)
                     /locus_tag="VLI90_18955"
     CDS             complement(2638..>4230)
                     /locus_tag="VLI90_18955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV16351.1"
                     /translation="AEEEERAHAGEAVDPAREWGSYAASMLLFVLAMLSKSSAFAMPL
                     VMMLLLWWKRRLSGRQVILLSPFVVIGTALALSAMDFERDTASTAGAGWDWSIAQRAI
                     IAGQAVWFYVGKLLVPTRLSFMYPKWAIDPANVMQYLPAAAAVVTIIWLLVVQRRFGR
                     GPIVAVLAFVLCLLPALGFVNIAPMRFTFVADHYAYLATVPLITLIVAAAVKALRRAR
                     AASAPPGPAIALAAILLVALGATSWVRAHVFISDLNLWADTVSKNPGSWFAHDRYGSA
                     LGVQSAVDQRIGDTDAYEAHLTEAMKQAKAAAELNPKDGEAQWIIGRVLIERRDYPAA
                     VDHLRQATQLDPKLSGAWQDLAATLVKQQHWQEAIEACDAALRVQPQSSPTHRLMAEA
                     YAGLNNLQRAIGENREALKIKPDNIVARENLADLLARTNQDKEAKLQYTSVLQQDHSR
                     PEVWNKLGVLYARGGDMGMAVQMFKAATQIQPDYQEANDNLKMAVSALEKQRQATAAT
                     RGSATQPGATTMPAGPTTGAAQ"
BASE COUNT          760 a         1497 c         1248 g          725 t
ORIGIN      
        1 ttccataggg tcggccgcgg ctaataggaa ttgcatgggg agttctctcg ctcgccgggt
       61 gtcttgcgct cgggtcgcgc ccgtgtcgtc ggggtcttta agatctttac tgcttcgtcg
      121 cgatcggcgc cgatcgcacc gctcgaatgc acgcgatcga cacgctgatc aacgttccgc
      181 cgtaaaacgc cagcagccaa tatgtgaacg gcttcgacgc cgtcaaggcg agcgccgccg
      241 ccgccgccga catcaggtgg atcatcgtcg ccaccagcgc cgcctgcgac atcgtcagct
      301 ggttcgcccc gcggcacagc agcagcggca gcatcgccag ttccgacgcc gccagcatca
      361 cgcccgcggc gatgtacatc tcgcgcatct gcgggttcac atccagcgct cgcgccgcga
      421 ccagcccgac gccggccatc accgcgatca ccagcggatt gaagatcaag gctcgaacca
      481 ccgcgcgcgt cgctttcgtg taatcgttac agaggcgtta acgtttgttg ctgcgcaccg
      541 actctttgat cagcgaatac atccccgcgg cacaaccgag catcacgccg gcgtaaacgc
      601 cccacggcgc ccagccgaag cgcttgtcgg cccagtggcc gatgaaataa ccgatgccga
      661 cacccacggc gacttccatg ccgagggcaa ggtttcggcc ccaatcgctg tcgtcatcac
      721 gggccatcgg tgcgctccgc gaagcaccgc acaaaagatc gtgcattttt gcacgaaccg
      781 gggcggtaaa ggtatgagaa gcacgctggc gcgtcaagaa gtgcatcgct acgcacagtc
      841 ccgtacgaaa ccccgcggat ctctatttcg cggccttttg cctttctttg tctttacgtt
      901 tactccgcct cattcaccgg cgctaccgcc gtcgtcggcg cagcgccgac gcgcacaatc
      961 cgcagcgcca tccacggcct ccccggcaac gcaattttcc gctccccctc cgccgcgaac
     1021 gcatacatcc cctgcttcac cagccgaacc gccggtccct ccaccggcgt gctcgtcatg
     1081 ttccacgtgt ccagcacatc cacccgaaac gtcatcccct cttccaaccc aatccgtggc
     1141 aattgaaacg cccactccgc cggccgctcg ctgccgaagt acaccagata aaactccccc
     1201 gcgcgcccgc cgacgtgcgt ttcataaaac cggtcgatcg gcgacaccgg cggcgcttcc
     1261 gtcaagatcg acttcaaaaa cgcaatccgc gccgcgctct gcccgcgcag ttcgccgccg
     1321 cggctggtcc acgattcacc actctggtcg cgcagcactt caccgtgacc gacgaacgtc
     1381 ccgccgatcg tgccgagcca gcagtgctcg accatctgct cggcggacag ctgcccccat
     1441 cgccgttcga tgttcccttc gtatcgcgcc tcgtcgtaga tgaccggctt gcccgccacg
     1501 tcgcgataaa gcgacgcccg accgaaatca tcgaccgccg ccccgttctg cacgctcagg
     1561 tgtgtgatcc acggcttgct cgaatcgtac atccgcgcgc tgaagtgaat ggaccgcagg
     1621 tgatccgccg gatcgtccgc ctgcaggatt tgaaagagcc gatcgaaatc gacgtcgtgc
     1681 ttctcgtgca tcaggtcgaa ctcgttcgcc atcgaccacc acacgttgcg atacgccgac
     1741 aggcgcgcga cgaggtaatg gacatagcga tcgtcgtcgc ccgcgggcat gcggtcgaag
     1801 cccatcgtgc ccttgtcgta tggatgaaac aggatcacgt ccgcttcgac ccccagctcg
     1861 cccagctgcg caatgcgctg ctccaggtgc cgaaaaaacg cgacgttgaa gcgcgcatga
     1921 tcgaacgcgc cgtcggccga tcgctcaaac ggaaaccgcg gcggctcgtc accgcctttt
     1981 ttgtacgccg tcggaaacac gcacatgcgc accttgttga acggcgacgc cttcagcgtc
     2041 gccagcgtgc gctgctcgag ggcctccggt tgatgcaccc acgcataaca agtcgtcccg
     2101 atcggcacga acgccgtccc gtccgcgtaa gcgaagtgat accggcccgc cacccgcacc
     2161 ggcccgtgat tcccgggcga cggcgccgta acggtgagtt ctccagcgcg accattcaac
     2221 tccgcccgat tgctccgcgt cacgaatcgc caaacgccga ccgccggcgg catgaagcgg
     2281 atgcggtaga caccgtcgcc gtcataaaat ccgcgcacgg ttcgagcgat ctcgttccag
     2341 gtgaacgttg ccgacagctc aacgtcaaga aatggattcc cctcgcgcgg ccccggcagc
     2401 gcgacttcaa agcgccccca ctgctcgacc ttcaacggca cggacgccgg ctgcgaagct
     2461 ggcgccgcgg caagcgaaca cgcgatcaat acgataagga tgaacagatg catgaatgcc
     2521 gtcaccacgc ggggttcgga gcgtactcgc gacgacccgg atctgccgcg catcgagcaa
     2581 gaagaatccg ggtcgtcgga gtacctccga acccggcgtg tcaggtcccg tgcgtattca
     2641 ttgggcggcg cccgttgtcg gcccggccgg catcgtcgta gcgcccggtt gcgtcgcgct
     2701 gccgcgggtg gcggctgttg cctggcgctg cttctccagc gcgctgaccg ccatcttcag
     2761 gttgtcgttg gcttcctggt agtccggttg aatctgggtg gcggctttga acatctggac
     2821 cgccatgccc atgtcgccgc cgcgggcgta caagacgccc agcttgttcc acacctccgg
     2881 gcgactgtgg tcctgttgca gcacgctggt gtactgcagc ttggcttcct tgtcctggtt
     2941 cgtgcgcgcg agcagatcag ccaggttctc gcgggcgacg atgttgtccg gcttgatctt
     3001 cagcgcctcg cggttctcgc cgatcgcgcg ctgaaggttg ttcaaaccag cgtacgcctc
     3061 ggccatcagc cggtgcgtcg gcgatgactg cggttgcacg cgcagggccg catcgcaggc
     3121 ttcgatcgct tcctgccagt gctgctgctt caccagcgtg gcggccaagt cctgccaggc
     3181 gccggacagc ttggggtcca gctgcgtggc ctggcgcagg tgatcgaccg cggcgggata
     3241 gtcgcgccgt tcaatcaaca cccggccgat gatccactgc gcttcgccgt ctttcggatt
     3301 cagttcggcc gcagccttgg cctgtttcat cgcctcggtg agatgcgcct cgtacgcatc
     3361 ggtgtcgccg atgcgctgat cgactgcgct ctgcacgccc agcgcgctgc cgtatcgatc
     3421 gtgggcaaac caggagccgg ggttcttcga aacggtatcg gcccaaaggt tcaggtcact
     3481 gatgaagacg tgggcgcgca cccagctggt cgcgccgagc gcgaccagaa ggatggccgc
     3541 cagagcaatg gccggaccgg gtggcgccga agccgcgcgc gcccgccgca acgccttaac
     3601 agcggcggcg acgatcagcg tgatcaacgg caccgtcgcc aggtacgcgt aatgatcggc
     3661 cacgaacgtg aagcgcatcg gcgcgatgtt gacgaacccc agcgccggca gcaggcacag
     3721 gacgaacgcc aagacagcga cgatcggccc gcggccgaag cggcgctgga cgaccagcaa
     3781 ccagatgatc gtgaccaccg cggcggcggc gggcaggtat tgcatcacgt tcgccggatc
     3841 gatcgcccat ttcggataca tgaacgacag ccgggtggga accagcagct tgccaacgta
     3901 gaaccacacc gcctgcccgg cgatgatcgc gcgctgggcg atgctccagt cccaccccgc
     3961 gccggcggtg gacgcggtgt cgcgttcgaa gtccatcgcc gacagcgcca gcgccgtgcc
     4021 gatcacgacg aacggcgaca gcaggatcac ctgccggccg ctcagccggc gcttccacca
     4081 cagcagcagc atcatcacca gcggcatcgc gaaggcgctg gacttgctca gcatcgccag
     4141 cacgaacagc aacatcgacg cggcatacga gccccactcg cgcgccggat cgacggcttc
     4201 gccggcgtgg gcgcgctctt cttcctcggc
//
LOCUS       DATIMH010001097         5264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1708570, whole genome shotgun sequence.
ACCESSION   DATIMH010001097 DATIMH010000000
VERSION     DATIMH010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708570"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VLI90_18960"
     CDS             complement(<1..305)
                     /locus_tag="VLI90_18960"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16352.1"
                     /translation="MSDRTEHGPPDLISVARAIQILDDVTVTPRVQRVRLADAQGLRL
                     ADQISSDRDYPPFDKSVMDGYAVRAADVTSAGVELRVVETIAAGASAQRSLAAGEAIA
                     "
     gene            893..1633
                     /locus_tag="VLI90_18965"
     CDS             893..1633
                     /locus_tag="VLI90_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16353.1"
                     /translation="MEFAIPPQAASEAPDLAAAVPADGALAEADLEASLRAIDDGQIT
                     APTEPAAAQSATAESAEPAPPGLSPGVACTPATTAADSTTPPPVAAASAASAPPSVTL
                     APVAPAALAPAARTRVVATRVPPLELPSELAYLRDLDCAQLWALLDEEQTKVTRWLLW
                     GEGPTWAAQKTGVARSKIYRWKAEPLFTAVYELLKRHKLAEIHDNAQMLGSRAIRALA
                     AGLDIETPKSAKLALELLKELGMFKRQE"
     gene            1699..2118
                     /locus_tag="VLI90_18970"
     CDS             1699..2118
                     /locus_tag="VLI90_18970"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSV16354.1"
                     /translation="MPATHVIFYAERNVSPFLRWLDDQQQKVQDKIFDAVTRLEALGF
                     ELRRPEADLLRDGIYELRVKKGHVNYRPLYFFDDETDPKTRKLIRRRAVIAHGCTKKG
                     RVDATDIDLAIRRRTNYLANRRDHTYVPPPAADAEQP"
     gene            2170..2502
                     /locus_tag="VLI90_18975"
     CDS             2170..2502
                     /locus_tag="VLI90_18975"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSV16355.1"
                     /translation="MAKRNANIPSASRILFDRLYRGKPRRIAALNQTRRELSLGRQIR
                     QLRESRGVSQAQLARALRTHAPAISRIEDADYDGHSLRVLRKIAEYFGQELVVTFKPQ
                     RGAATTAN"
     gene            2543..3115
                     /locus_tag="VLI90_18980"
     CDS             2543..3115
                     /locus_tag="VLI90_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16356.1"
                     /translation="MGTLNAIYIRSTDAAAARAIRMNYSSAVTEPGTRFFAVELPADS
                     FRCPEAELQQLSKRLQTDVIWLSFQSVADAFQYYHWQSGKRVRALAYGCFVRDGEWEQ
                     AEGQPQEWEAAVLFNRDRVHLEHYTPAEQKQLKRIFREQILKVGSPYPSLDARETARG
                     VAEYYRFPGWELSDEDDDQYDDEDDNWDDE"
     gene            3413..4387
                     /locus_tag="VLI90_18985"
     CDS             3413..4387
                     /locus_tag="VLI90_18985"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSV16357.1"
                     /translation="MPSGQTSLEFVGIGDPGNAADTKVMSDGTTGYGAVSYSYQLGKF
                     DVTAAQYSQFLNAVAKSDPNGLYDTNMANGFLGPCGIVRSGSSGGYTYSVLSGHQNLP
                     VNFVSWGDAARFVNWLTNGQPATGVEDSATTEMGSYSLSGATSDAQLIAVARSAAAKY
                     VIPTEDEWYKAAYYKGGSANAGYWLYPTRSNTAPSNILSASGTDSANYFSSAYTDPTN
                     YITAVGAFAGSPGPYGTFDQGGDVWQWNEANIDGSHRGVRGGSFSNYLNGATYLQSSS
                     RNILDDPSVSGSNLGFRIAEVPEPASISVLALGIAALVRRRRSSSRVN"
     gene            complement(4419..>5264)
                     /locus_tag="VLI90_18990"
     CDS             complement(4419..>5264)
                     /locus_tag="VLI90_18990"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSV16358.1"
                     /translation="IAKKHPEVFVEGGIMERGNFSAAAGFGYTKGKQGIFSTFSAFLE
                     MCISEITMSRLNFANVLAHFSHSGVDDMADNTCHYGINSMFADGGVTPSHGEDMTRLY
                     FPADQHQFAACVKRIYGDPGLRFLFSTRSAVPDILDTDGKPMYQGKPFEPGKDYLVRE
                     APAGGGYVVATGEIVYRALDAVIRLQEQGVKVGLVNKPTLNVVDESMMKRLASAPFVL
                     VTEGWNVKTGLGSRFGSYLLKAGFKGKYNNIGTYREGCGGLWQQMGFQGVDSEGIAKA
                     IKALA"
BASE COUNT         1033 a         1650 c         1571 g         1010 t
ORIGIN      
        1 gcgatcgctt caccggcggc gagcgaacgc tgtgcgctcg cccccgcggc gatcgtttcg
       61 acgacgcgaa gttcgacacc cgcgctggtg acatcggcag cgcgcacggc gtagccgtcc
      121 atcaccgact tgtcgaacgg cgggtaatcg cgatcagatg aaatctgatc cgccaggcgc
      181 aacccctgtg catccgcaag ccgcacgcgc tgcacgcgcg gcgtgaccgt cacgtcgtcc
      241 agaatctgaa tcgcccgcgc aacactgatc aaatccggcg ggccgtgctc ggtgcgatcg
      301 gacatgccgc tatcgtggtg cacgcccgca gggccgtcaa tcgccggcag cgggcgggtc
      361 tgactcttaa tctttacgtt gtaagcgcca aggccgccaa ggcgccaaga gcgccaagaa
      421 cgaaaagaca tttcgccacg gatgaacgca gatgtacgca gatcaatcca aagtgatttc
      481 atctgcgagc atctgcgttc atctgtggca caccctccgg ctgtttcctc ttggcgctct
      541 tggcgactcg gcggccttgg cgcgatcttc aaaagccaaa accttccgga acctggcatg
      601 gcacctgcgc cgtggggcgc gttggggcgc gccgatgcgc aggcttttgt ccggtcattt
      661 tctataagcc tcattcacgg catatcttac taagacatca gcacccatgt ttagcaaaga
      721 tgtactacgt tgcgcccaag attttgccaa gacctcccat ttaacgtgca ttactaaatg
      781 aaatttcgcg attttaatct tgcacaggcg acgtttgtta ggtataagtt tggcatatcc
      841 tctaaccgaa ggagccgata tgcatcgttc ttcgctcgat aacaatctgc caatggagtt
      901 cgccattccg ccgcaggcgg cgtcggaggc gccggatctg gcggccgccg tgccggccga
      961 tggcgccttg gccgaagctg atcttgaagc gtccttgcgg gccatcgacg acggccagat
     1021 caccgccccg accgaacccg ccgccgcgca atccgccacc gccgaatccg cggagccggc
     1081 tccgccgggt ctctcgcctg gggtcgcctg cacccccgcc acgacggccg cagactcgac
     1141 caccccgccg cccgttgccg ccgcgtccgc ggcgtccgcg cctccgtcgg tcacgctggc
     1201 gccagtcgcg ccggcggcgc tcgcgccggc ggcacgcacg cgggtcgtgg cgacgcgcgt
     1261 cccgccgctg gagctgccgt cggaactggc ttacctgcgc gaccttgact gtgcccaact
     1321 ctgggcactg ctcgacgaag aacagaccaa ggtgacgcgc tggctcctct ggggcgaagg
     1381 ccccacctgg gccgcccaga agaccggcgt cgcccgatcc aagatctacc gctggaaggc
     1441 cgaaccgctg ttcaccgccg tttacgaact gctgaagcgt cacaagctcg ccgagattca
     1501 cgacaacgcg cagatgctcg gcagccgagc catccgcgcc ctcgccgccg gcctcgacat
     1561 cgagacccca aaaagcgcca aactcgcgct ggaactgctg aaggagctgg ggatgttcaa
     1621 aaggcaagag tgaggcgcgc cgtgagttcc ctagatttgc attaacaaat ttgttataat
     1681 agcatttccg gccagcgaat gccggcaacc catgtcatct tctacgccga gcgaaacgtc
     1741 agcccgtttc ttcggtggct ggatgatcaa cagcagaagg tgcaggacaa aatttttgac
     1801 gccgtcacgc ggctggaagc cttgggcttc gaattgcgcc gacccgaggc cgacctgctg
     1861 cgtgacggca tttacgagct acgcgtcaag aagggccacg tgaactatcg gccgctgtac
     1921 ttcttcgacg acgagacgga cccgaagacc aggaagttga tccgcaggcg cgcggtgatc
     1981 gcgcatggtt gcacgaagaa aggccgcgtc gatgccaccg acatcgacct ggcaattcgg
     2041 cgccgcacca actacctcgc caaccgccgc gatcacacgt acgttccacc gccggcggcg
     2101 gatgcggagc agccgtaacc gctccccgtc atcaaatgtt ccgtcggcaa tcttgaagga
     2161 agtagtctga tggcaaagcg aaacgcgaac atcccatcgg caagccgaat cctgttcgac
     2221 cgtctctacc gcggcaaacc gcggcggatc gcggctctga accagacgcg gcgcgagctg
     2281 tccctcggcc ggcagatccg ccagctccgc gaatcgcgcg gcgtatcgca ggcgcagctc
     2341 gcccgcgcgc tgcgcacgca cgcgcccgcc atcagccgca tcgaagacgc cgactacgac
     2401 ggccacagcc tccgcgtcct gcggaagatc gccgagtact tcggccagga actggttgtc
     2461 acgttcaaac cgcaacgcgg ggcggcgacc actgccaact gacttgggcg tcgcataatg
     2521 cgatcacaag catccgacca ccatgggaac gctcaacgcc atctacatca gatccaccga
     2581 cgccgcggcc gcacgcgcta ttcgcatgaa ctattcctca gcggtcacgg aaccgggcac
     2641 ccgattcttc gccgtggaat tgcccgccga tagcttccgc tgtcccgagg cagaactgca
     2701 acaactgtcg aagcgtctgc agacggatgt catctggctc agcttccaaa gcgtggccga
     2761 tgccttccag tattatcact ggcaaagcgg caagcgcgtc cgcgcgttgg cgtacggctg
     2821 cttcgtccgc gatggcgaat gggaacaggc tgaaggccag ccgcaggagt gggaagccgc
     2881 cgtgctcttc aaccgtgatc gcgttcacct ggagcactac accccggcgg aacagaagca
     2941 gctgaagcga atcttccgcg agcagatcct gaaggtgggc agcccgtatc ccagcctcga
     3001 tgcccgcgaa accgcccgcg gcgtggcgga gtattataga ttcccgggat gggaactgtc
     3061 cgatgaggac gacgatcaat acgacgatga ggatgacaat tgggacgacg agtaggccac
     3121 gctgaatcga cgccgacggt gaactaaacg ttcacgtgcc tgctcccgcg ggttcgcagc
     3181 caatccattt tccattgctt gcgatatttc cagcggcgct tgacgccttt ctttgcgctg
     3241 gataaaagtt ggtagtgctc atcacggggg aactgtgcga tgcgatcatc catttcaatt
     3301 taaacggttg ggcaaactca tgaaagtcac acgagggcaa gccacttcaa ttgcggcatg
     3361 ggttttcgcc atagccttga gcgctggcac gtgtcgatcg agcgtcttgg cgatgccgtc
     3421 cggccaaacg agtctcgaat tcgttggcat cggagacccc ggtaacgccg ccgacacgaa
     3481 ggtaatgagc gacggcacta ccggatacgg cgcagttagt tacagctatc aattgggcaa
     3541 gttcgatgtt actgcggcgc agtactctca attcttgaat gcggtggcca aatcggatcc
     3601 gaatggattg tacgacacga atatggccaa cggcttcctt ggcccgtgcg gcatcgtcag
     3661 gtcagggagc tcgggtggtt acacatacag cgttctaagc gggcatcaga acttgccggt
     3721 caactttgtg agttggggtg atgctgcccg cttcgtgaac tggctgacga acggtcagcc
     3781 cgcaactggc gttgaagaca gcgctacaac cgaaatgggt tcttattccc ttagcggcgc
     3841 gacgtccgat gcgcagctca ttgccgtcgc tcgctcggcc gcggccaaat atgttattcc
     3901 caccgaggac gagtggtaca aggccgcgta ctacaagggt ggttcggcca acgcgggtta
     3961 ttggttgtac cccacgcgca gcaacaccgc cccgagcaat attttgtccg cgagtggaac
     4021 cgatagcgcg aattacttca gcagcgcgta caccgatcca accaactaca taacggcggt
     4081 cggcgccttc gccggttccc cgggcccata tggcacattt gatcaagggg gtgacgtctg
     4141 gcagtggaat gaagccaata ttgatggatc acatcgaggg gtgcgtggcg gctcgttcag
     4201 caactacctc aatggtgcga cttatctcca gtccagctct cgcaacattc tcgacgaccc
     4261 ctccgtcagc ggcagcaatc ttggcttccg gattgcggag gtgccggagc cagcatcaat
     4321 atccgtgctg gcattgggga tcgccgcatt ggttcgcagg cggcgttcat cgtctcgggt
     4381 taactaggct ggtttgatcc gcgttcgtgc gtcggcgttt acgccagcgc cttgatcgcc
     4441 tttgcgatcc cttccgaatc cacgccctga aagcccatct gctgccacag gccgccgcag
     4501 ccttctcggt aggtgccgat gttgttgtac ttgcctttga atccggcttt cagcaggtag
     4561 ctgccgaagc ggctgcccag gccggtcttg acgttccagc cttcggtgac gagcacgaac
     4621 ggggctgatg cgaggcgctt catcatcgat tcatcgacga cgttgagcgt gggcttgttg
     4681 accaggccga cttttacgcc ttgctcttgc aggcggatga cggcgtcgag cgcgcggtag
     4741 acgatttcac cggtggcgac gacatatccg ccgccggcgg gggcttcgcg gacgaggtag
     4801 tccttgccgg gttcgaaggg cttgccctgg tacatcggtt tgccgtcggt gtcgaggatg
     4861 tcgggaacgg ccgatcgggt gctgaacagg aaccgcaggc cggggtcgcc gtagatgcgt
     4921 ttcacgcagg cggcgaactg gtgctggtcg gcggggaagt agaggcgcgt catatcctcg
     4981 ccatggctcg gcgtgacgcc gccgtcggcg aacatgctgt tgatgccgta gtggcaggtg
     5041 ttgtcggcca tgtcatcgac gccgctgtgg ctgaagtgcg ccagcacgtt ggcgaagttc
     5101 aggcgcgaca tggtgatctc gctgatgcac atttccagga atgcgctgaa cgtcgagaag
     5161 atcccctgct tgcccttggt gtaaccaaac ccggcggcgg cggagaagtt gccgcgctcc
     5221 atgatgccgc cttccacgaa cacttccgga tgcttcttcg cgat
//
LOCUS       DATIMH010001098         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_841510, whole genome shotgun sequence.
ACCESSION   DATIMH010001098 DATIMH010000000
VERSION     DATIMH010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841510"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VLI90_18995"
     CDS             complement(<1..390)
                     /locus_tag="VLI90_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16359.1"
                     /translation="MALLFPIALLAPAGLAQPVTTAPAAATQPVAGAGNEQAIAAELQ
                     QNIGKMRELLGGIDGLIDPAKRAKIAPDIVPVLKRLIANFDELVAAQPMAKEQAAGAK
                     QQFLVFLAVFGDTDANNQLKQMAESKDP"
     gene            552..625
                     /locus_tag="VLI90_19000"
     tRNA            552..625
                     /locus_tag="VLI90_19000"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:586..588,aa:Cys,seq:gca)
     gene            complement(642..1076)
                     /locus_tag="VLI90_19005"
     CDS             complement(642..1076)
                     /locus_tag="VLI90_19005"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HSV16360.1"
                     /translation="MDLQSRLQEDMKTALKAGQKDRLQVIRMLLSDVKNIDLMPNKPT
                     AEQAVEAYAKKLRKSIDEYQKLGKPDEVQKLQFEIGVAEEYLPKRASAEETDQLVEAF
                     LAQNSFTEKQAGQATGMFMKANAGKVDAALASAAIRKRLAGT"
     gene            1500..2597
                     /gene="rho"
                     /locus_tag="VLI90_19010"
     CDS             1500..2597
                     /gene="rho"
                     /locus_tag="VLI90_19010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HSV16361.1"
                     /translation="MGTNGHVEGVLSVTDDRHDGELRDPTHPLRPVRGNVIVPRNLIR
                     DLHLRPGLLLKGSPRGHVLDRIETIEGRSPDEYVEKLPLYDSTALDPQPVIKLEHSPT
                     EPTTRVIDILTPVGFGQRGLIVAPPRTGKTILMQNIAKGIHANYPNVQLVLLLVDERP
                     EEVTDMKRNVPGTVYASSNDNTVEKHLDLAQIVIERCKRQVEFGADIVVLMDSLTRVG
                     RAFNTGGVQGGRTMSGGLDNRALEVPKRLFGAARKIEHGGSLTILATCLIDTGSRMDQ
                     VIFEEFKGTGNMELTLDRNLANQRIWPAINIAESGTRKEEKLLDEKQLAATRTIRRHL
                     MNMSPAAAMKSLTDALAKHKTNQSLYDSMRT"
     gene            2806..>3343
                     /locus_tag="VLI90_19015"
     CDS             2806..>3343
                     /locus_tag="VLI90_19015"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSV16362.1"
                     /translation="MRVLISGAGGFLGSHLTDLLLSHGHSVVGVDNFITGKPQNVAHL
                     EGNKKYKLIRHDVIDPVKIDGPVDRIYHMASPASPIGYVKHQVATVKVNSQGTWNLLE
                     LSERKSSRFLMASTSECYGDPAVNPQPESYWGNVNSIGLRSMYDEAKRFSEACTMAYH
                     RERGVDTRIIRIFNTYGPR"
BASE COUNT          638 a         1025 c         1038 g          642 t
ORIGIN      
        1 cgggtccttg ctctcggcca tctgcttcag ctggttgttc gcgtcggtat caccgaagac
       61 ggcgaggaag acgaggaact gctgtttggc gccggcggcc tgttccttgg ccatgggctg
      121 ggccgccacg agttcgtcga agttcgcgat gagccgcttc aggaccggca cgatatcggg
      181 cgcgatcttg gctcgcttgg ccggatcgat cagcccgtca atgccgccga gcagctcgcg
      241 catttttccg atgttctgct gcagttcagc ggcgatggcc tgttcgttgc cagcgccggc
      301 caccggctgc gtcgccgccg ccggggcggt ggtcaccggt tgggccaatc ccgcgggcgc
      361 cagcagcgcg atcgggaaaa gcaatgccaa tgggagcaac cggcgccgcg tgcgtgaatt
      421 cgtagggatc ataagcggta agttcctcgg tttgccgcgt caacggcgtc tgcaatggta
      481 gaccctcgcg cccggcaagt cctgcgcgac ttgatggaat tgcgctggcc ctatacagtc
      541 aatcgaagct cggccgagta cccaagtgga ctaaggggac ggattgcaaa tccgttattc
      601 gtcggttcga atccgacctc ggcctttgcg gttgggcggg cttatgtgcc ggccaatctc
      661 ttgcggatgg cggcgctggc gagggcggcg tcaaccttgc cggcgttggc cttcatgaac
      721 atgccggtcg cctggccggc ttgtttctcg gtgaagctgt tctgggccag aaacgcttcg
      781 accagctggt cggtctcttc ggcggaggcc cgcttgggca ggtattcctc ggcgacgccg
      841 atctcgaatt gcagcttctg gacttcgtca ggcttgccga gcttctggta ctcatcgatg
      901 ctcttgcgaa gcttcttcgc ataagcttcg accgcctgct cggcggtcgg tttattgggc
      961 atcaggtcga tgtttttgac atcgctcagc agcatgcgga tgacctggag gcggtccttt
     1021 tgaccggcct tcagggccgt cttcatgtct tcctgaagtc gcgattgcag atccatcttt
     1081 tactccgttg gtgatttcgg ttatcgtacc gattccaccg agggcgacca gagcttgagt
     1141 ttatcctgta gcggaggcgg tactccgcca gagtcgaagg atctggcatc aaattcggtg
     1201 aagcgcagcc tctgcgagag attgcggtcg ctggcgaaaa tccagttcac tgcatcctga
     1261 gctatatcct tcgactgcgc ggctgcgtcg cttcgctcag gacaagcatt cacggacgac
     1321 aacaaatgaa cgaacgccgg cggttcggga accggcccca tcgctcgggg cagtcacggt
     1381 ccaacaagcc ccataatagg aaccctcagc agcaatcgca caatcgatcg cagcatcagt
     1441 ccccgcacgc gcatcgctca tcgcaatccc atcagacgca accgcaagcg ggtggaccgg
     1501 tgggaacgaa cgggcatgtg gaaggcgtgt tgtcggtgac cgatgaccgc cacgatggcg
     1561 agctgcgcga tcccacgcat ccgcttcgcc cggtgcgcgg caatgtgatc gtgccgcgga
     1621 acctcatccg cgatctccat cttcgcccgg gcctattgct gaagggcagc ccgcgcgggc
     1681 acgtgctgga tcggatcgag acgatcgaag gccggtcgcc cgatgagtac gtggagaagc
     1741 tgccgctgta cgactcgacg gcgctcgacc cgcagccggt catcaagctg gagcacagcc
     1801 cgaccgagcc gacgacgcgg gtgatcgaca tcctcacgcc ggtcggattt ggccagcgcg
     1861 ggttgatcgt cgcgccgccc aggacgggca agacgatcct gatgcagaac atcgccaagg
     1921 ggattcacgc gaattacccg aacgtgcagt tggtcctgct gttggtcgac gagcggccgg
     1981 aagaagtgac cgacatgaag cgcaacgtgc cgggcacggt gtacgcttcg agcaacgaca
     2041 acacggtcga gaagcacctg gatttggcgc agatcgtcat cgagcgctgc aagcggcagg
     2101 tggaatttgg cgctgatatc gtcgtgctga tggattcgct gacgcgcgtg ggtcgcgcgt
     2161 tcaacaccgg cggggttcaa ggcggccgga cgatgtccgg cggtttggac aaccgcgcgc
     2221 tggaagttcc taagcgtctc ttcggcgctg ctcgaaagat cgagcatggc ggatcgctaa
     2281 cgatcttggc gacttgcctc atcgacaccg gttctcgcat ggatcaggtg atcttcgaag
     2341 aattcaaagg caccggcaac atggagttga cgctggaccg caaccttgcc aaccagcgca
     2401 tctggccggc catcaacatc gccgaatccg gcacgcgcaa agaagaaaaa ctgctggacg
     2461 aaaaacagct ggcggcgacg cgcacgatcc gccggcacct gatgaacatg tcccccgcgg
     2521 cggcgatgaa gtcgctgacc gatgcgctgg ccaagcacaa aaccaaccaa tcgctttacg
     2581 attcgatgcg gacgtagggc ggcgccgcaa gtcagatatt tcggcaggaa gtggaagaat
     2641 cccgcgccaa tgcggccctt atcggaccgc gcgctttccc gtatccttgg atgtctgtgc
     2701 tgtgcccgcc ggtgccggct tgcgataagt cttgcacttc aatcggccga tattaagcgg
     2761 cgtcggcctc tcgggcgcaa ctggcttctc cagaaggacg tttcgatgcg tgtgttaatc
     2821 agcggcgcgg gcgggttcct cgggtctcat ctcacggacc tgctgttgtc ccatggccac
     2881 agcgtggtcg gcgtggacaa cttcatcacc ggcaagccgc agaacgtcgc gcacctggaa
     2941 ggcaacaaga aatacaagct catcagacac gatgtgatcg acccggtgaa gatcgacggc
     3001 cccgtcgacc gcatctacca catggcatca ccggcgtcgc cgatcggtta cgtgaagcac
     3061 caggtcgcca cggtgaaggt caacagccag ggcacgtgga acctgctgga gctgtccgag
     3121 cgcaagagct cgcgcttcct gatggcgagc accagcgagt gctacggcga tccggcggtg
     3181 aacccccagc ccgaatccta ctggggcaac gtcaactcca tcggcctgcg ctccatgtac
     3241 gatgaagcca agcgcttctc cgaagcctgc acgatggcct accaccgcga gcgcggcgtc
     3301 gacacccgca tcatccgcat cttcaacacc tacggcccgc gca
//
LOCUS       DATIMH010001099         1748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2000060, whole genome shotgun sequence.
ACCESSION   DATIMH010001099 DATIMH010000000
VERSION     DATIMH010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000060"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..775)
                     /locus_tag="VLI90_19020"
     CDS             complement(239..775)
                     /locus_tag="VLI90_19020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV16363.1"
                     /translation="MNKKLNTTAQGGFTLIELIVVIVILGILAATALPKFSSLGGDAR
                     VATLNAARGSIASVVAMAHGKALVSGNSATPVTFENVQVALVNGYPSAAATTANAAGI
                     ETGPDFTVLTGANATAGTAGPVRTATQFAIVPTAVAGTANAANCYVLYTEATASAATP
                     PVITPPTVVTTATADNCQ"
     gene            complement(984..>1748)
                     /locus_tag="VLI90_19025"
     CDS             complement(984..>1748)
                     /locus_tag="VLI90_19025"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSV16364.1"
                     /translation="DHIEFDRDMRGRVKSALRYPMFVCIAMLVAMLVVNIFVIPQFAK
                     VFQSFHAELPLMTRILIAGSNFTKEYWYVIIGGLAGAVVAFTRWTATAAGSLVWDRWK
                     LRLPIAGKIIHKATMARFARSFALSIRSGVPIVQALAVVAQTADNTYLSMRMDQMREG
                     VERGESILRTATSADVFTPIVLQMIAVGEESGSLDDLMDEIAQMYEREVDYELKTLSS
                     QIEPIMISFLGVMVLVLALGIFLPIWDLGKAALHNK"
BASE COUNT          340 a          518 c          540 g          350 t
ORIGIN      
        1 gtccggaatc ggttgagctt attgcggtag acgcttgatt atattcaagc cctgccggaa
       61 atcgttaccg tttttgctga acgggaaaag aatatctggt tccgcagggc aatttaccga
      121 ttagcgttgt cggtacgccg cactcatcca ttcgaagaac gcgccgggcg atgcccaaaa
      181 aacaaatcgc ggcaggagcc gcgattcgcc aggctttccg gccaggccgg aatgcggatt
      241 actggcagtt gtcggcggtg gccgtcgtca cgacggtcgg cggcgtgatc acgggcgggg
      301 tcgcggcgct tgccgtcgcc tcggtataca agacgtagca gttggccgcg ttggcggtgc
      361 cggcgacggc ggtcggaacg atggcgaact gggtggcggt gcgcaccggt ccagccgtgc
      421 ctgcagtagc gttcgcgccc gtcagcacgg tgaaatcggg accggtttcg atgccggccg
      481 cattggcggt cgtggcagcc gcgctcggat aaccattgac cagggcgact tgcacgtttt
      541 cgaacgtgac cggggtggcg ctgtttccgg acaccagcgc cttgccatgg gccatcgcca
      601 ccaccgatgc gatcgaaccg cgcgccgcgt tcagcgtggc aacgcgggca tcgccgccca
      661 ggctggaaaa cttcggcagc gccgtcgccg ccaagatacc gaggatcacg atcacgacga
      721 tcaattcgat cagggtaaag ccgccctgcg cagtggtgtt caacttcttg ttcatcgatc
      781 tctccgagaa atgtcagcgg ttgaataccg cctgggtgca ttggtgtgcg gattcgtcat
      841 ttacggcatt aattattgcc aataagaatc cttgatacgt ggaaattcta ccttggattt
      901 gagcaaactt gtgccatgga aagacaaagt ttatcgatca ctgatgtatt cgataatttt
      961 ttgtcgcgcc agataaacac agcttatttg ttgtgcaacg cggctttgcc caggtcccag
     1021 atcggcagga aaatgcccag cgccagcacc agcaccatga cgcccaggaa cgagatcatg
     1081 atgggttcga tctggctgga cagcgtcttc agttcgtagt ccacctcgcg ctcgtacatc
     1141 tgcgcgattt cgtccatcaa atcgtccagc gagcccgatt cctcgcccac cgcgatcatc
     1201 tgcagcacga tcggcgtgaa cacgtcggca ctggtggccg tgcgcaagat gctttcgccg
     1261 cgttcgacgc cctcgcgcat ctggtccatg cgcatcgaca ggtaggtgtt gtcggcggtt
     1321 tgcgcgacca cggccagcgc ctgcacgatc ggcacgccgc tccggatcga cagcgcgaag
     1381 ctgcgcgcga agcgggccat cgtggctttg tggatgatct tgccggcgat cggcaggcgc
     1441 agcttccagc ggtcccacac caggctgccg gcggccgtgg cggtccagcg ggtgaatgcg
     1501 acgaccgcgc cggccaggcc cccgatgatg acgtaccaat actccttggt gaagttggag
     1561 cccgcgatca ggatgcgcgt catcagcggc agctcggcgt gaaagctctg gaacaccttc
     1621 gcgaactggg gaatgacgaa gatgttcacg accagcatcg ccaccagcat cgcgatgcag
     1681 acgaacatcg gatagcgcag cgcgctcttc acgcggccgc gcatgtcgcg gtcgaactcg
     1741 atgtggtc
//
LOCUS       DATIMH010001100         4933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1129268, whole genome shotgun sequence.
ACCESSION   DATIMH010001100 DATIMH010000000
VERSION     DATIMH010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129268"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1058
                     /gene="bioB"
                     /locus_tag="VLI90_19030"
     CDS             33..1058
                     /gene="bioB"
                     /locus_tag="VLI90_19030"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HSV16365.1"
                     /translation="MPLNPSVRHDWQPSEIRALYDTPVMELVYQAATVHRRFHDAGEI
                     QVCKLISIKTGGCPEDCKYCSQSSRYETEVDASPLMDKAEVLEIAQRAKAAGVTRVCM
                     GAAWREVRDNKQFDRVLDMVKGVNDLGVEVCCTLGMLTEDQAKKLEAAGLYAYNHNLD
                     TGREYYETIITTRTFEDRLKTIENVRKTSVTLCSGGIIGLGERIDDRLSMLHTLCTMN
                     PHPESVPINILSKVPGTPLAETPDVPFWETLKMIALARILMPKATVRLSAGRVNLSPS
                     EQALCFMAGANSFFSSETKVMLTKAVASPAYDEDKALLNTLGLTMRPPFKDEQSAPSH
                     APEPAVV"
     gene            1065..1496
                     /locus_tag="VLI90_19035"
     CDS             1065..1496
                     /locus_tag="VLI90_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16366.1"
                     /translation="MSDDNAWQDGRSQTASSCLARGRSLSNEGRYDEAIDSFLAAINA
                     DPDLLEAHEALWETALLRKAAGEPALGLFETMKLRRPTGSEKQRLLNALKQLAYDPTD
                     FECVSDAFQSATKLELTHVTPWLTKMLRKMNADSSKPEPSL"
     gene            1493..2707
                     /locus_tag="VLI90_19040"
     CDS             1493..2707
                     /locus_tag="VLI90_19040"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HSV16367.1"
                     /translation="MNAVPVITGIGLVTPLGRSVAETWASILAGQSIRTHARVETDAG
                     SNTPRVARLALHAAHEAVEQSGWRTRPIAPWRTAIIVGTSKGPIESWIAPPLAAASPS
                     DKPVNPAMASPVFGLASVAEALASQFEMPEAPRLTLSAACASGLHALIRGAMMIQSGE
                     VTRVLVVAAEASAHPLFLGSFRRLGVLPPEGVPCRPFDQNRQGFLMSDAAAAVCLEAV
                     TSDADPTASPIAAIERFAMAGDAKHITAGDPTGAVMRHLLRAVLGNRPVDLIHAHGTG
                     TALNDEIELSAIEHVLACDRPTTNSTSDNPCYVKFNPGGRAGGSQVAVYSHKGAMGHS
                     LGASGLVSVVLNCVAHRTGIVPPNVNTMEPIATRWTSISRSAVHRPVRRSLACATGFG
                     GPTSVVSLISVK"
     gene            complement(2714..3913)
                     /locus_tag="VLI90_19045"
     CDS             complement(2714..3913)
                     /locus_tag="VLI90_19045"
                     /inference="COORDINATES: protein motif:HMM:NF023462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3524 domain-containing protein"
                     /protein_id="HSV16368.1"
                     /translation="MPKQLDILALEPFFGGIRRNMLETIIRCSRHRWTLLKLPPRRIE
                     RRLAAAAHWFSEQLSRHWVGHVDLLFTSEALNLADLYRLTPNLARKPAVVYFHSNELP
                     EPGVTSVNPALDYANLNTAAAATEIWFNSKFHVRMFLAGVNALVTANTEFAAQNPMPA
                     LTVKIRHMPPPVDLSIARELTAQQPPPVRGKRTVFVETRDANIDLLNAGLGILQRRGE
                     KVQLITVGPVEHLTTEVPRQTLPENDVVGQSRAILESTIFASTKIAATFDEHAVRALA
                     LGCRTVMPRTGVYPELLPKAMHGSCLYDIGADSLASRLQDALYGPANVPGAQDELERS
                     LHTFDLITICKLIDERLSELSEAHTTSRIARAEKIAAKDAAANKAAPAPRPDGKNLQP
                     PRAPRPG"
     gene            complement(4067..>4933)
                     /locus_tag="VLI90_19050"
     CDS             complement(4067..>4933)
                     /locus_tag="VLI90_19050"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV16369.1"
                     /translation="THTLFTRANQAVPNNYLAQCLLSGYLRDEKRIDDALAMSHEAAV
                     TAPNSGGAHRQYALSLQVAGRRKEALHELRLAVQLNPTDFNVFNDIGGVLVEEGRDDQ
                     ALEMFQRAVAMEPDFAMARHNLAVMLANKGRMDEAIAHWRVAEQLEPANGIVHGFLGE
                     ALRLRGDRRGAVTEYAAMWASKDHRPEWETNYAWLVATDPISSPEEIDLAVGAARDAC
                     TSAPADQKALAQDALAAALARAGQFNDAGAAAQQGVDLANAAGQPALAKAITARSALY
                     RAGQPYFEQPKR"
BASE COUNT          886 a         1594 c         1582 g          871 t
ORIGIN      
        1 acgacgcgtt cacagctgga gcacgataga cgatgccgtt gaatccatcc gtccgccacg
       61 actggcagcc gagcgaaatc cgggcgctgt atgacacgcc cgtgatggag ctggtgtacc
      121 aggccgccac cgtacatcgc cgttttcatg acgcgggcga gatccaggtc tgcaagctga
      181 tctcgatcaa gaccggcgga tgtcccgaag actgcaagta ctgttcgcaa tcctcccgct
      241 acgaaaccga agtcgacgcc tcaccgctga tggacaaggc cgaggtgctg gagatcgccc
      301 agcgcgccaa agccgccggc gtcactcgcg tgtgcatggg cgctgcctgg cgcgaggtgc
      361 gcgacaacaa gcaattcgac cgcgtgctcg acatggtcaa gggcgtgaac gacctgggcg
      421 tcgaagtctg ctgcacgctc ggcatgttga ccgaagatca agcgaaaaag ttagaagcgg
      481 cggggctgta cgcctacaac cacaacctcg acaccggccg ggaatattac gagacgatca
      541 tcacgacgcg cacgttcgag gaccgcctga agacgatcga gaacgtccgc aagaccagcg
      601 tcaccctctg cagcggcggc atcatcggcc tgggcgagcg catcgacgac cgcctgtcga
      661 tgctgcacac gctctgcaca atgaacccgc atccggagtc ggtgccgatc aacatcctga
      721 gcaaagtccc cggcaccccg ctggcggaaa cgccggacgt gccgttttgg gaaacgctca
      781 agatgatcgc gctcgcccgc atcctgatgc ccaaggccac ggtccgcctg tcggcggggc
      841 gggtcaacct gtcaccgagt gaacaagccc tctgcttcat ggccggcgcc aattccttct
      901 tctccagcga gaccaaggtc atgctgacca aggccgtcgc gtcgccggcg tacgatgagg
      961 acaaggcgct gctgaacacg ctgggcctga cgatgcgtcc gccgttcaag gacgagcaat
     1021 ccgcgccttc ccacgcgccg gaacctgcgg tggtttgagt cgccatgtcc gacgacaacg
     1081 cctggcaaga cggtcgttcg caaaccgcgt catcgtgcct cgcccgcgga cgatccctca
     1141 gcaacgaagg ccgctacgac gaggcgatcg actcgttcct cgccgccatc aatgctgatc
     1201 cggatctgct cgaggcgcac gaagcgctgt gggaaaccgc attgctgcgc aaggcggcgg
     1261 gggagccggc gctgggttta ttcgagacga tgaagttgcg gcgaccgacc ggcagcgaaa
     1321 agcagcgact cctcaatgcg ctgaagcagc tcgcgtacga cccgaccgat tttgaatgcg
     1381 taagcgatgc gtttcagtca gcgacaaagc tggagttgac gcacgtcaca ccctggctga
     1441 ctaagatgct gcggaagatg aacgctgact catcgaagcc ggagccttcg ctgtgaacgc
     1501 tgttcccgtc atcacaggaa tcgggctcgt tacgccgctc gggcggagcg tcgccgagac
     1561 ttgggcctcg atcctcgccg gccagagcat ccgaacgcac gcgcgggttg agaccgatgc
     1621 cgggtcgaac acgccgcgcg ttgcgcggct tgcgttacac gccgcgcatg aagcagtcga
     1681 acaatccggc tggaggacgc gaccaatcgc gccatggcga acagcaatca tcgtcggcac
     1741 cagcaagggc ccgatcgaaa gttggatcgc cccgccgctg gctgccgctt cgccgtccga
     1801 taaacctgta aatcccgcga tggcatcgcc agtgttcgga ttggcatcgg tcgccgaggc
     1861 tctcgcgagt cagtttgaga tgcccgaagc gccacggctg acgctctccg ccgcgtgcgc
     1921 cagcggactg catgcgctga tccgcggcgc gatgatgatt caatccggcg aagtgacccg
     1981 ggttctcgtc gtcgccgccg aagcatcggc gcatccgctg ttcctcggca gctttcgccg
     2041 cttaggcgtc ctaccaccgg aaggcgtgcc gtgccgcccg ttcgatcaga accgccaagg
     2101 cttcctcatg agcgacgccg ccgccgcggt ttgcttggaa gccgtaactt cggacgctga
     2161 tccaaccgcc agtccgattg ccgcgatcga acgattcgcg atggccggcg acgcgaagca
     2221 catcacggcc ggcgatccaa ccggcgcggt gatgcggcat ctgctacgtg cagtccttgg
     2281 aaatcgtccg gtggacctga tccacgccca cggcaccggt actgcgctga acgacgagat
     2341 cgagctgagc gcgatcgaac acgtgctggc ctgcgaccgc cccaccacaa attcaacgtc
     2401 cgataatcca tgttatgtta agttcaaccc tggaggacgg gcagggggtt cacaggtcgc
     2461 ggtttactcg cataaaggcg caatgggcca cagtctgggc gcttcgggct tggtgtcggt
     2521 cgtgttgaac tgcgtggccc accgcaccgg gattgtgccg ccgaacgtga acacgatgga
     2581 gccgatcgcg acgcgatgga cgagcatctc gcgctcggcc gtgcatcggc cggttcggcg
     2641 aagcctggcg tgtgcaacgg ggtttggcgg gccgaccagc gtcgtcagcc tgatcagcgt
     2701 gaaatgacca gggtcatccc ggccgcgggg ctcgcggggg ttggaggttc ttgccgtcgg
     2761 gccgcggcgc gggcgcggct ttattggccg ccgcatcttt cgcggcgatt ttctcggcgc
     2821 gagcgattcg actggtcgtg tgggcttcgc tcaactcgct caggcgttcg tcgatcaact
     2881 tgcagatcgt gatcaaatca aacgtgtgca ggctccgttc caactcgtct tgcgcgccag
     2941 ggacgttcgc ggggccatat agagcatcct gtaatcggct ggcgagcgag tcggcgccga
     3001 tgtcgtacag gcacgagcca tgcatcgcct tcggcagcaa ctcagggtaa acgccggtgc
     3061 gcggcatcac cgtccggcag cccaacgcca gcgcccgcac ggcatgttca tcgaacgtcg
     3121 ccgcgatctt cgtgctggcg aagatggttg attccaagat ggcccggctc tggccaacca
     3181 cgtcgttctc gggcagcgtc tgccgcggca cctcggtggt cagatgctcg accgggccga
     3241 ccgtgatcag ctgcaccttt tcgccgcggc gttgcaggat gcccaggccg gcgttgagca
     3301 agtcgatatt cgcgtcgcgc gtttcgacaa agacggtccg cttgccgcgc acgggcggcg
     3361 gttgctgggc ggtcaattcg cgggcgatgc tcaagtccac cggcgggggc atgtgacgga
     3421 tcttgaccgt cagcgccggc atcggatttt gcgccgcgaa ttccgtgtta gcagtcacca
     3481 acgcgttcac gccggccagg aacatgcgga cgtggaactt actgttgaac cagatttccg
     3541 tggccgcggc ggcggtgttc aggttggcgt aatcgagcgc gggattgacc gacgtcacgc
     3601 ccggttcggg cagctcgttg ctgtggaaat agacgacggc gggcttgcgc gccaggttcg
     3661 gcgtcagccg atatagatcc gccagattga gcgcttcgga cgtgaacagc agatcgacat
     3721 gccctaccca atgtcgcgac aattgctcgg aaaaccaatg ggcggccgcg gcgagccggc
     3781 gttcgatccg ccgcgggggc agcttcagca gggtccagcg atgccggctg cagcggatga
     3841 tcgtctcgag catgttccgc cggatgccgc cgaaaaacgg ctccagcgcg aggatgtcga
     3901 gttgcttggg caatgtgatg ttgcgtaaag cgcggcacgc gatgcacgct cacctgaccg
     3961 agtcgaacgg gcgagcggca ccatagaaca tcatccggct ttgaatggta ttcgcaagcg
     4021 cacgcccgcg cgcccgcgcg cgcgatcacg cggcgcatcg tgaccgtcag cgcttcggtt
     4081 gttcgaagta cggctgaccg gcgcgataca gggccgatcg agcggtgatg gctttggcca
     4141 gcgcgggttg accagcagca ttcgccagat ccacaccctg ctgcgccgca gcgccggcgt
     4201 cgttgaactg ccccgcccgc gccagcgccg ccgccagcgc gtcttgcgcc agtgcctttt
     4261 gatcggccgg cgcggacgtg catgcgtcgc gcgctgcacc gaccgctaga tcgatttcct
     4321 cgggtgacga tatcgggtct gtcgcaacga gccacgcgta gttggtttcc cactcgggcc
     4381 gatggtcttt gctggcccac atggcggcgt attcggtgac cgcgccacgg cggtcgccgc
     4441 gcaggcgaag cgcctcgccg aggaacccgt gcacgatgcc gttcgccggc tccagttgct
     4501 cggcgacgcg ccagtgggca atcgcctcgt ccattcgccc cttgttggcc agcatgaccg
     4561 caaggttgtg ccgcgccatg gcgaagtccg gctccatcgc gacggcgcgc tggaacattt
     4621 ccaacgcctg gtcatcgcgc ccttcctcga ccagcacgcc accaatgtcg ttgaacacgt
     4681 tgaagtcggt cgggttcagt tgcaccgcca gccgcagttc gtgcagcgcc tctttgcgtc
     4741 gccccgccac ttgcagcgac agggcatact ggcgatgcgc cccgccgctg ttcggcgccg
     4801 tgacggccgc ttcgtgcgac atggccagcg cgtcatcgat gcgcttctca tcgcgcaaat
     4861 agcccgagag caggcattgc gccaggtagt tgttcggcac ggcttggttc gctcgcgtga
     4921 agagcgtgtg ggt
//
LOCUS       DATIMH010001101         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2282264, whole genome shotgun sequence.
ACCESSION   DATIMH010001101 DATIMH010000000
VERSION     DATIMH010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2282264"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..>2298)
                     /locus_tag="VLI90_19055"
     CDS             complement(39..>2298)
                     /locus_tag="VLI90_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16370.1"
                     /translation="VLAALAAALVIYVYRRDGRSRAVRISLGILRGGLLLLVLLLLNR
                     PVLTLGQSRTEPSVLAIMVDDSISMRVRDAGDEHDPQSRLQAAENLLSDHDAELLRTL
                     SQRHALRFYRFDCDAQPISALDPSSANAQPAVEALQKLEPTGQSTQVLPSILSVMQDL
                     QGQRVAGVVVMTDGRDTPTHAVGSALDTLKNYGVKVYPLAIGSDRQPRNIEVQGVTVE
                     DTAFKGDIVNVKASVRASGFEANHPVQVVLKDKKTGAQLPGIDGSPAERTINLPDDKP
                     VEVELQWKTSDVGSHDVIVEAVRQPGEVDDDDNVRTAQVAVLDAKISVLYVDGYPRWE
                     YRYLKNEMVRDKTVDISCLLSSADPGFAQEGDKPIRRFPESIEELMDYDVVVFGDVDP
                     RFFTDTQLQLVSEFVSRKGGGFAMIAGPRFAPQAYRNTAIEPVLPVAISRVEGTDSNA
                     TITQGFRPVLTRVGQASSIFRFFADRQQNENFLKNDIQPIFWYCRGVTVKPGVGEVYA
                     EHPTDVAPDGRKAPILVLGRFGAGRTLFSSIDDSWRWRFYTGESVFDTYWVQQLRYLA
                     RSRKIGQRKVTLTAGRPVYELGGQVRLTLRILDPVLLQQLPEQIRVEVRDANGQPVRF
                     ENLMRQEGQSDLYTGSFTADRVGKFTVQLPPIAGGVDMMEVPLEISLPRLELIDPQVD
                     RTQLSRLASETLGKAIDLSNAKTELPQIPSAAKVIPVVSGQPLWDAPLTMGLFVLLIA
                     TEWVLRKVYGMV"
BASE COUNT          402 a          778 c          701 g          417 t
ORIGIN      
        1 gccgcgaggc cgcggcggct aaacggcatg ccgtatcgct acaccattcc atacaccttg
       61 cgcaacaccc actccgtcgc gatgagcaac acgaacaggc ccatcgtcag cggcgcgtcc
      121 cacagcggct gacccgacac gaccggaatc accttcgccg cactggggat ctgcggcagc
      181 tccgtcttcg cgttgctcag gtcgatcgcc ttccccagcg tctcgctcgc cagccggctc
      241 agctgcgtgc ggtccacctg cgggtcgatc aattccagcc gcgggaggct gatctccagc
      301 ggcacttcca tcatatccac cccaccggcg attgggggga gttgaaccgt gaatttgccc
      361 acacgatcag ccgtaaacga gccggtgtag aggtcgcttt ggccctcctg ccgcatcagg
      421 ttttcgaacc gcacgggctg gccgttggcg tcacgcactt cgacgcggat ctgctcggga
      481 agttgctgca acaagaccgg atcgaggatg cgcagcgtga gtcgaacttg cccgcccagt
      541 tcgtacaccg gccggcccgc cgtcagcgtc accttccgtt ggccgatctt tcggctccgc
      601 gccaggtaac gcaattgctg cacccaatac gtgtcgaaca cgctttcgcc ggtgtagaag
      661 cgccagcgcc acgaatcgtc gatcgacgaa aagagcgtgc gacccgcgcc gaatcttccg
      721 agtaccaaaa tcggcgcctt tcggccatcg ggcgcgacat ccgtcggatg ctccgcgtac
      781 acctcgccga cgcccggctt gacggtcacg ccgcggcagt accagaagat cggttggatg
      841 tcgttcttca aaaagttttc gttctgctga cgatcggcga agaagcggaa gatgctcgac
      901 gcctgcccca cccgcgtgag caccgggcga aacccctgcg taatcgtcgc gttcgaatcg
      961 gttccttcga cgcgcgaaat cgccaccggc agcaccggtt cgattgccgt gttccgatac
     1021 gcctgcggcg caaaccgcgg gccagcgatc atcgcgaacc cgccgccctt tcggctgacg
     1081 aattcgctga ccagctgcag ctgagtgtcg gtgaagaagc gcgggtcgac gtcgccgaag
     1141 acgaccacgt cgtaatccat cagctcttcg atgctctcgg gaaaccgccg gatcggcttg
     1201 tcgccttcct gcgcgaagcc ggggtcggcg ctgctcagca ggcaggaaat atccaccgtc
     1261 ttgtcgcgca ccatctcgtt tttgagatag cgatactccc agcgcgggta accatcgacg
     1321 tacagcacgc tgatcttcgc atccagcacc gcgacttgcg ccgtgcggac gttgtcgtcg
     1381 tcatccacct cgccgggctg gcgcaccgct tcaacgatga catcgtgcga gccgacgtcg
     1441 ctggtcttcc actgcagttc gacctcgacc ggcttgtcat cgggcagatt gatcgtgcgc
     1501 tcggcgggcg agccgtcgat gccgggcagc tgcgcgccgg tcttcttatc tttaaggacg
     1561 acttgaaccg ggtgattcgc ttcaaacccg ctggcgcgaa cgctggcttt gacgttgacg
     1621 atgtcgccct tgaacgcggt gtcttcgacg gtgacgccct gcacttcgat gttccgcggc
     1681 tggcgatcgg acccgatcgc cagcgggtac accttcacgc cgtagttctt cagcgtgtcc
     1741 agcgcgctgc cgacggcatg agtcggtgta tcacggccat cggtcatcac gacaacgccg
     1801 gcgacacgct ggccctgcag gtcctgcatc accgacaaga tcgacggcag cacctgcgtg
     1861 ctttggccgg ttggctccag cttttgcagc gcctcgacgg ctggctgagc gttcgcgctg
     1921 ctgggatcaa gcgcgctgat gggctgggca tcgcaatcga atcgataaaa ccgcagcgcg
     1981 tgccgctggg aaagcgtgcg cagcaattcc gcgtcgtgat cggacagcag gttctccgcc
     2041 gcctgcaggc gcgactgcgg gtcgtgttca tcgccggcgt cgcgcacgcg catgctgatg
     2101 ctgtcgtcga ccatgatcgc cagcaccgac ggctcggtcc ggctctggcc cagcgtcagc
     2161 acgggacggt tgagcagcag cagcacgagc agcagcagcc cgccgcggag gatgccgagg
     2221 gagatgcgca cggcgcgaga acggccgtcg cgccggtaga cgtaaatcac cagcgccgcc
     2281 gccagcgccg cgaggacg
//
LOCUS       DATIMH010001102         5757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4593773, whole genome shotgun sequence.
ACCESSION   DATIMH010001102 DATIMH010000000
VERSION     DATIMH010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4593773"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VLI90_19060"
     CDS             <1..401
                     /locus_tag="VLI90_19060"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSV16371.1"
                     /translation="KLVNGKDFSTSPWKGKVVLVDFWATWCGPCMEEMPRVKKLYAQY
                     HPKGLEMLGVSNDYSADDLKKFIADNTDAPWPQLFDPNAAQQQKWNPITEGFGIQGIP
                     TMFLIDKKGTVRTVNARENMEDLIPKLLAE"
     gene            complement(525..1571)
                     /locus_tag="VLI90_19065"
     CDS             complement(525..1571)
                     /locus_tag="VLI90_19065"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSV16372.1"
                     /translation="MRWMTCIIYGLVAACCGVVVAAGAPAPATQALAGEVNQLSMGDW
                     GTDGRGQQAVARAMADYVERTNKNVSGMLLVGDNFYMKLPGGVNDPMWQSAFERMYDP
                     ARLNFPFFAALGNHDYQFGKDVIELDYAKTHPASRWKLPARWYRVDIPVDHPLVTVLM
                     LDSDKDPLGPAQWAEQRAWLEAELGKPRGTWTMCCAHHPLFSNGGHGDNGVLQNDWGS
                     LFVKYKVDFYVCGHDHDLQHLQIPNWFTSFVLVGGGGQDTKLMRHDDRGPMSRLTHGF
                     AHFEITPESAVVRYVNDEGKVIHQFTRDKGGRVTVQIEGGHDKAAGNPLRALEGFGGF
                     KPPVTTQPAGAAGD"
     gene            1726..3108
                     /locus_tag="VLI90_19070"
     CDS             1726..3108
                     /locus_tag="VLI90_19070"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSV16373.1"
                     /translation="MRPFTPLLAVMCLGTVCLGTVALAANPAGMVDTNAPTGVNLRRT
                     VTVQVVEKTKNAVVYISTTKLVNQRVPMGINPFFENFDSGPMVRVPANSLGSGFIVHR
                     DGYVVTNNHVIDRARHIDVELADGRKLPADLISTDPDADLAILRVRSDSPLPTLELGE
                     SNDLMIGEPVIAVGNPLGFSHSVSTGIVSAVHRDIDPRRKTANANDAIKGEEAALRDL
                     IQTDAAINPGNSGGPLLNAYGQVIGINTAIRGDAQNIGFAIPIDRLRDLIPELMNPAQ
                     VTKVDVPLKLAEKRTMTEPATVNAAVTSAGSTTKVLDTINGQHPQDIVDAYAMLLRCK
                     VNEPLKVTWANGKTETIKPRATPTPDAIVQARQKLGLTIEQVTPMLAEKCHLSTEDGL
                     FVSAVDRGSIAAKAGLAPGDVVISLGPYPVRSLVDLATLLPQLPESGRVRIGVVRGEQ
                     MGVGLLEFNQ"
     gene            3169..4329
                     /locus_tag="VLI90_19075"
     CDS             3169..4329
                     /locus_tag="VLI90_19075"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HSV16374.1"
                     /translation="MKIVIAPDKFKGCLNAADVAEAIAVGARSVDASLALDLCPMADG
                     GEGTVAALVAATGGRLVTRRVVGPLSEMSVDATFGFLGDDVTAVIEMSAASGLALLKP
                     QDRNPLNTTTFGTGQLIAAAIGEGASQIILGIGGSATIDAGIGCAQACRFTLLMRDGE
                     LTSMTEPLCGRDLPNVLMVKHGRGEITNGVQITVACDVTNPLFGDNGAARVFGPQKGA
                     SPADVERIDADFLAFAERTGKMAEAQSPGAGAAGGLGFGMTAFFGATLRSGVDLVADA
                     VGLRGRLRGASLCITGEGQLDGTSAHGKTVGGVARLCRELGVPCIALAGSIGDGAEQM
                     ISEGLTAHFSICDRPMTMNESCRDARTLLSRVAANVMRAARSSPPPATLPLK"
     gene            4326..4619
                     /locus_tag="VLI90_19080"
     CDS             4326..4619
                     /locus_tag="VLI90_19080"
                     /inference="COORDINATES: protein motif:HMM:NF021402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HSV16375.1"
                     /translation="MKFGRIKRQHSRVGGLDEILNRIVKQCPHVSRIVPGRIKVRRGK
                     TPPNFKVQYPTSAGLKCLYTATGTVQEVFLICDNAPAAKAWLIESGIATAEND"
     gene            4824..>5757
                     /gene="cax"
                     /locus_tag="VLI90_19085"
     CDS             4824..>5757
                     /gene="cax"
                     /locus_tag="VLI90_19085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HSV16376.1"
                     /translation="MRYVNYFCYLAIPLAFVSHFWIGHGKDWEPIATFLLAGIGVIPL
                     AHLMGTATEQLAEETGPTLGGLLNATFGNAAELIIGVIAITKGLNDVVKASLTGSILG
                     NLLLVSGAAMVVGGWNREKQTFSREALQANAGLLVLGVAGMLFPAIFHFTYRLHDVDL
                     LEHEHGVSIGTSIILLIVYGLGLLFTLKTHAHIFSRGPATSPEDPIGISGVHERWSVK
                     KSVLMLLLASAGIAIVAELLVGSAAAVAERMGWNRIFVGVMLLAIIGNAAEHSTAVLL
                     ARRNDMDTAMTIAHQSSLQIALFATPFLVLLSSAM"
BASE COUNT         1090 a         1957 c         1710 g         1000 t
ORIGIN      
        1 ggaagctggt taatgggaag gatttttcga cgtcgccgtg gaagggaaaa gtcgtgctcg
       61 tggacttctg ggccacctgg tgcggcccgt gcatggagga aatgccgcgc gtgaagaagt
      121 tgtacgcgca gtatcacccc aagggcctcg agatgctcgg cgtgtcgaac gattactccg
      181 cggacgacct gaagaaattc atcgctgaca acaccgacgc cccttggccg cagctgttcg
      241 atccgaacgc cgctcagcag cagaagtgga acccgatcac cgaaggcttc ggcatccaag
      301 gtatcccgac gatgttcctg atcgacaaga agggaactgt ccgcaccgtg aatgcgcggg
      361 aaaatatgga agacctgatc ccgaaattgc tggcggagta gtgaccttcc aacacccccc
      421 acgccgggtt cggagcgtac tcgcgacgac ccggattctt ccctgccatt gagacaacaa
      481 atccgggtcg tcggcgtacg ttcgaaccca gcgtggggaa ggattcaatc ccccgccgca
      541 ccggccggtt gcgtggtcac cggcggctta aacccgccga agccctccag tgcccgcagc
      601 gggttgcccg cggctttatc gtgcccgccc tcgatctgca ccgtcacccg gccgcccttg
      661 tcgcgcgtga actggtggat cactttgccc tcatcgttca catagcgcac gaccgccgat
      721 tccggcgtga tctcgaaatg cgcgaaccca tgcgtcaagc gcgacatcgg cccgcggtcg
      781 tcgtggcgca tcagcttcgt gtcttgtccg ccgccgccga ccaagacgaa gctcgtgaac
      841 cagttgggga tctgcaggtg ctgcaggtcg tggtcgtgcc cgcagacgta gaagtccacc
      901 ttgtacttca cgaagagcga tccccaatcg ttctgcaaca cgccgttgtc gccgtgcccg
      961 ccgttgctga acagcgggtg atgggcgcag cacatcgtcc aggtgccgcg cggcttgccc
     1021 agctcggcct ccagccacgc gcgctgctcc gcccactgcg ccggcccgag cggatcctta
     1081 tcgctgtcca gcatcagtac agtcaccagc ggatgatcga cgggaatgtc cacacgatac
     1141 caacgcgccg gcagcttcca gcgcgacgcc gggtgcgtct tggcgtagtc cagctcgatc
     1201 acgtccttgc cgaattgata atcgtgattg cccagcgccg cgaagaacgg gaagttcaga
     1261 cgagccggat cgtacatgcg ctcgaacgcc gactgccaca tcggatcgtt caccccgccg
     1321 gggagcttca tgtaaaaatt gtcgccgacc aacagcattc cgctgacgtt cttgttggtc
     1381 cgttcgacat agtccgccat cgcgcgggcc accgcctgtt ggccgcggcc atcggtgccc
     1441 cagtcgccca tcgacagctg gttcacctct cccgccaaag cctgcgtggc cggcgccggc
     1501 gcgcccgcgg caacaacgac accgcagcac gctgcaacta acccataaat aatgcatgtc
     1561 atccagcgca tggacacgtc gatcctccat tgccacaaaa cggctcgggc acacaatatc
     1621 agacgctgca aggttatact gccgagactg gctgcccgtt ctaaccgcag cctgcgattc
     1681 tcgtctattc tggcagaaac tcagtctaag attcccaaca tgatcatgcg accattcacc
     1741 ccattgctcg ccgtgatgtg cctcggcact gtgtgcctcg gcaccgtcgc ccttgccgcc
     1801 aaccccgcgg ggatggtgga cactaacgcc ccaaccggcg tcaacctgcg ccgcacggtg
     1861 acggtgcagg tcgtcgagaa gacgaagaac gccgtcgtct acatctcgac gacaaagctg
     1921 gtgaaccagc gcgtgccgat gggaatcaac cccttcttcg aaaatttcga cagtggaccg
     1981 atggtgcgcg tgcccgccaa ttcgctgggc agcggattca tcgtgcatcg cgacggatac
     2041 gtcgtcacca acaatcacgt gatcgaccgc gcgaggcaca tcgacgtcga gctggccgac
     2101 ggccgcaagc tgcccgcgga tttgatcagc accgatcccg atgccgacct ggccatcctg
     2161 cgcgtgcgca gcgattcccc gctgcccacg ctggagttgg gggaatcgaa cgacctcatg
     2221 atcggagagc cggtcatcgc cgtcggcaat ccgctcggct tcagtcacag cgtcagcacc
     2281 ggcatcgtct cagcggttca tcgcgacatc gaccctcggc gcaagaccgc caacgccaac
     2341 gacgccatca aaggtgaaga agccgccctt cgcgacctga tccagacgga cgccgccatc
     2401 aatcccggta acagcggcgg tccgctgctc aacgcctacg gccaagtgat cggcatcaac
     2461 accgccatcc gcggcgacgc gcagaacatc ggcttcgcca ttcccatcga tcgcctgcgc
     2521 gatctgatcc ccgagctgat gaaccccgcg caggtgacga aggtggatgt cccgctgaag
     2581 ctggcggaaa agcgcacaat gaccgagccg gccacggtga atgccgcggt cacctctgct
     2641 ggttctacca caaaggtatt ggatacgatc aacggccagc atccgcagga catcgtggat
     2701 gcctatgcga tgctgctgcg ttgcaaggtg aacgagccgc taaaggtgac atgggctaat
     2761 gggaagacgg agacgatcaa gccgcgcgcc acgccgacgc cggacgcgat cgttcaagcc
     2821 cggcaaaagc tcgggctgac gatcgagcag gtcacgccga tgctggcgga gaagtgtcac
     2881 ctgtccacgg aggacgggct gttcgtcagc gccgtcgatc gcggcagcat cgccgcgaaa
     2941 gccggcctcg cccccggcga tgtcgtgatc agcctcgggc cttaccccgt gcgctcactg
     3001 gtcgatttgg ccacgctgct cccgcagctg cccgaaagcg gccgcgtccg catcggtgtc
     3061 gtccgcggcg aacagatggg cgtggggttg ctcgagttta atcagtagca cggacgccac
     3121 agcccgtgct gaggtgcgct gacatgggct ctggcgtccg tgctacagat gaagatcgtc
     3181 atcgcccccg acaaattcaa aggctgtttg aacgccgccg acgttgctga ggcgatcgcc
     3241 gttggcgcgc ggtctgttga cgcatcgctg gccctcgacc tctgccccat ggccgacggc
     3301 ggggaaggca ccgtcgccgc cctcgtcgcc gccacgggtg gccgcctggt tacccgccgc
     3361 gtcgtcgggc cgctgtcgga gatgagcgtt gacgccacct tcggtttcct cggcgacgac
     3421 gtcaccgccg ttatcgaaat gtccgccgcc agtgggctcg ccctgctgaa accgcaggat
     3481 cgcaatccgc tcaacacgac gacgttcggc accggtcaat tgatcgccgc cgccatcggc
     3541 gaaggggcga gccagatcat cctcggcatc ggcggcagcg cgacgatcga cgccggcatc
     3601 ggttgcgccc aggcctgccg gttcacgctg ctcatgcgcg acggcgagct cacgtcgatg
     3661 accgaaccac tctgcggccg cgatctgccc aacgtcctca tggtcaaaca cggccgcggc
     3721 gagatcacca atggcgttca aatcaccgtc gcgtgcgacg tcaccaaccc cctcttcggc
     3781 gacaacggcg ccgcccgcgt ctttggtccg caaaaaggcg catcgcccgc ggacgtcgaa
     3841 cggatcgacg ccgatttcct cgccttcgcc gagcgcaccg ggaaaatggc cgaagcgcaa
     3901 tcccccggcg ccggcgctgc gggtgggctc ggcttcggca tgaccgcgtt cttcggcgcg
     3961 acgctgcgca gcggcgtcga tctcgtcgcc gacgcggttg gtcttcgcgg ccgcctgcgc
     4021 ggcgccagcc tgtgcatcac cggcgaaggc cagctcgacg gcaccagcgc gcacggcaaa
     4081 accgtcggcg gcgtcgcgcg cctctgccgc gaactcggcg ttccctgcat cgccctcgcc
     4141 ggctccatcg gcgatggcgc cgagcaaatg atcagcgaag gcctcaccgc gcacttctcc
     4201 atctgcgatc gcccgatgac gatgaatgaa tcctgccgcg atgcgcgaac gctgctgtcg
     4261 cgcgttgccg ccaatgtcat gcgagccgcg cgatcttcac cgccgcctgc tacactgccg
     4321 ctcaagtgaa attcggccgc atcaaacgtc agcacagccg cgtcggcggg ctcgatgaga
     4381 tcctcaatcg catcgtgaag cagtgcccac acgtctcgcg catcgtcccc ggccgaatca
     4441 aagtccgccg cggcaagacc ccgccaaact tcaaggtgca gtaccccacc agcgccggac
     4501 tgaaatgcct ctacaccgcc accggcacgg tgcaggaggt tttcctgatc tgcgacaacg
     4561 cccccgccgc caaggcgtgg ttgatcgaat ccggcatcgc gacggcggag aatgattgaa
     4621 agcgccgaac aactggctaa taatcgccgc ggagacacgg agactcggag actcggagac
     4681 acggagacac ggagataaca agttgaaaga aagcccacgg ctcttccgtg ggaccgtttt
     4741 cgaaaagccg gtcccacggc agtcaagatc cttcgactgc gcacgcgacg cgcgtgcggc
     4801 aatcaggata cactctcatt cgcatgcggt acgttaatta cttctgctac ctcgccatcc
     4861 cgctcgcgtt cgtctcgcat ttctggatcg gccacggaaa agattgggaa ccgatcgcga
     4921 cgttcctgct tgcgggaatc ggggtcatcc ctctcgctca tctcatgggg accgcgacgg
     4981 agcagctcgc cgaagaaacg gggccgaccc tcggtggcct gctcaacgcc accttcggca
     5041 acgccgccga gttgatcatc ggcgtcatcg ccatcaccaa gggcttgaac gacgtcgtca
     5101 aagcgtcgct gaccggatca atcctcggca acctgctgct ggtctcgggc gcggcgatgg
     5161 tcgtcggcgg gtggaaccgc gagaagcaga cgttcagccg cgaagcgctg caggcaaacg
     5221 cggggctgct cgtgctcggc gtcgcgggca tgctcttccc cgcgatcttt catttcactt
     5281 accgtttgca cgacgtcgat ctgctcgagc atgaacacgg cgtctcgatc ggcaccagca
     5341 tcatcctgct gatcgtctac ggcctgggcc tgctcttcac gctgaagacg cacgcgcaca
     5401 tcttctcgcg cggcccggcg accagcccgg aagatcccat cggtatttcc ggcgtacacg
     5461 agcgatggtc ggtgaagaaa agcgtcctca tgctgctgct ggcgtcggca gggatcgcga
     5521 tcgtcgccga attacttgtg ggttccgccg ccgccgtcgc ggaaaggatg gggtggaacc
     5581 gcatcttcgt cggcgtgatg ctgctggcga tcattggcaa cgccgccgag cattccaccg
     5641 ccgtcctgct ggcgcgccgc aatgacatgg acaccgccat gacgatcgct caccaaagca
     5701 gcctgcagat cgcgctgttc gccacgccgt ttttggtgct gctgagttcc gcgatgg
//
LOCUS       DATIMH010001103         1844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5466536, whole genome shotgun sequence.
ACCESSION   DATIMH010001103 DATIMH010000000
VERSION     DATIMH010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5466536"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VLI90_19090"
     CDS             <1..670
                     /locus_tag="VLI90_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16377.1"
                     /translation="SLFRVLAGALGGFALGLGTIVLLRDVTDATLAFALFGVVVGELA
                     RRRKSVAFAVQSVAWIVLAAVGGVWNFPTTIAIVVAAVVVIARVEERFARGTALAVAA
                     AGALVCAVYAAVHLIQPSDPMALALIRTVSLAAVAAALALVARFSWAAGAIGLARAVL
                     VIGGLQLLIEDVRIGSALMLVAAFAAYGSAMVVVGRCALRHTDPKQAIQVTEPGEQTM
                     QSAN"
     gene            683..>1844
                     /locus_tag="VLI90_19095"
     CDS             683..>1844
                     /locus_tag="VLI90_19095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HSV16378.1"
                     /translation="MKQHHSIRTTIATAILALVMLPLAASAATRRAVTPPASTSPAPA
                     KASRYTPSQLEAYLSDDGIAYIRPGFHITVNSVTIGSDRKPVVDLSFTDDLNQPLDRL
                     GQTTPGPLSLSFTVGWYNPATRQYTNYITRTATAAAPSKQIGTTATQAATDSGGKFVD
                     LATGHATYTYGTALPANFDQTKTHTVGIYATRNLTAQISKSYFANAEFDFRPDGNKVT
                     EKWDKINQAASCNNCHDPLSAHGGARQDVKLCVQCHQPQSTDPDTGNTVDFKVLIHKI
                     HNGSNLPSVKAGTPYQIIGFGQSVNDFSTVVFPQNVLNCQNCHEGTNASAKPSQSSVY
                     FTNPSRAACGSCHDNINWTTGANHPGGAQADDAACATCHIPDSGQEFDASIKG"
BASE COUNT          332 a          655 c          557 g          300 t
ORIGIN      
        1 ttcccttttt cgtgtgttgg caggcgcgct cggcggcttc gcactcggcc tcggtaccat
       61 cgtgctgtta cgcgacgtca cggatgcgac gctggcgttc gccctcttcg gtgtggtggt
      121 gggggaactg gcacgccgtc gaaagtcggt cgcctttgcc gtccagagcg tcgcgtggat
      181 cgtcctcgcc gcggtcggtg gcgtgtggaa cttccccaca acgattgcga tcgtcgtggc
      241 ggcggtcgtc gtcatcgcca gggtggagga gcggttcgcc cgcgggacgg cgctcgccgt
      301 tgcggcagca ggtgctttgg tatgcgcggt ttacgcggca gttcatctga tccagccgtc
      361 ggatccgatg gctctggcgc tcatccggac cgtgtcgctg gcggccgtcg cggcggcgct
      421 ggcgctcgtc gcacgcttct cgtgggcggc cggtgcaatc ggactggccc gagcggtcct
      481 cgtcatcggc ggtctccaat tgctcatcga agacgtccgg atcggctccg cgctgatgct
      541 cgtcgcggcc tttgcggctt acggcagcgc gatggtcgtc gtcggtcgat gtgcgcttcg
      601 tcacacggac ccaaagcaag cgatacaggt caccgagccg ggtgagcaaa caatgcaatc
      661 agcgaattag aggagaccat cgatgaaaca acaccacagc attcgcacga cgatcgcaac
      721 cgcgatcctc gctcttgtga tgcttccgct tgccgcgtcc gccgccaccc ggcgcgccgt
      781 gacgccgccg gcgtccacgt cgccggctcc ggcgaaggct tcccgctata cgccgagtca
      841 gctcgaggcg tatctgagcg atgacggcat cgcctacatc cgccccggct tccacatcac
      901 cgtgaattcg gtgacgatcg gcagcgaccg caagccggtc gtcgacctgt cgttcaccga
      961 cgatctcaac cagccgctcg accgcctcgg ccagacgacg ccgggcccgc tgtcgctcag
     1021 cttcacggtc ggctggtaca accccgccac gcggcaatac acgaactaca tcacccgtac
     1081 ggccacggcc gccgcgccga gcaagcagat cggtacgacc gcaactcagg cggccaccga
     1141 tagcggcggc aagttcgtcg atctcgccac gggccacgcg acgtacacct acggaaccgc
     1201 gctgccggcg aacttcgacc agaccaagac ccacaccgtc gggatctacg ccacgcgcaa
     1261 cctcaccgcg cagatctcga agagctactt cgcgaacgcc gaattcgact tccgccccga
     1321 tggcaacaag gtcaccgaga agtgggacaa gatcaaccag gcggcgagct gcaacaactg
     1381 ccacgaccca ctgagcgccc acggcggcgc gcgccaggac gtcaagctct gcgtccagtg
     1441 ccaccagccg cagtccaccg atcccgacac cggcaacacg gtcgatttca aggtgctgat
     1501 ccacaagatc cacaacggct cgaacctgcc gagcgtcaag gccggcacgc cgtaccagat
     1561 catcggtttc ggccagtcgg tcaacgactt ctcgaccgtc gtcttcccgc agaacgttct
     1621 caactgccag aactgccacg agggaacgaa cgccagcgcg aagccgtcgc agtcgagcgt
     1681 ctacttcacg aaccccagcc gcgccgcttg cggttcctgc cacgacaaca tcaactggac
     1741 gaccggcgcc aaccatccgg gcggcgcaca ggccgatgac gcggcatgcg ccacctgcca
     1801 catccccgac agcggccagg agttcgacgc gtcgatcaag ggcg
//
LOCUS       DATIMH010001104         3464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3729644, whole genome shotgun sequence.
ACCESSION   DATIMH010001104 DATIMH010000000
VERSION     DATIMH010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3729644"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VLI90_19100"
     CDS             complement(<1..1169)
                     /locus_tag="VLI90_19100"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSV16379.1"
                     /translation="MKFSRREFGRLALAGVPATLLVKPGSAILAAADKPNSKWAGVQV
                     GMNVPYNFGTGNYTSGDEILQRCVQLGVSAVELRAQPVELFLGSPAAIAGAANQGRGR
                     GGRRGAAAAGDASAGVGAAAGAGAGTGAGAGAGAGAGAAAGAAAAGGGAAERGAGAGQ
                     GAAGAEGRGRGRGPLTPEQEAAQKAAAEETRKWRAGVSLDKVKEFRKKYEDAGVLIEI
                     VKWDGIFGFSDDELDYAFQVSKALGARALSSEISVGQTKRLGQFADKHKMPVGYHGHT
                     AVTPAIWEATFAEAKYNAANVDLGHFVAGNNTSPVPFIKQYHDRITHVHVKDRKLNNG
                     PNVPFGQGDTPIKEVLQLMRDNKWKFQATIEFEYPVPAGSDRMTEIAKALQYCKD"
     gene            complement(1220..>3464)
                     /locus_tag="VLI90_19105"
     CDS             complement(1220..>3464)
                     /locus_tag="VLI90_19105"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSV16380.1"
                     /translation="KKDLVCDCYGTELANVEHNENSLLWAMDNWMHTSEGDTYFRYKD
                     GKMETRKTLSRGQWGATQDDFGRVYRNSNSSALHIDLLSTPYFLRNPNLLRTRGSYEF
                     MGDPDELNATYPVRPNKGVNRGYQTGQLRGDGTLATYTGVNSPMVYRGDRLPAELAGN
                     VFIAEPTGNLISRIIVSDDGTTLRGKKAYANAEFMAATDERFRPVYLSSAPDGTIYVV
                     DIYHGIIQEKGFITEYLRDHIVAHKLETPIHMGRIWRIVHDTTRRDGPPALGSESATQ
                     LVGRLSHPNGWWRDEAQQLLIQRGDRSVVPVLKKLAASSTEPRTRVHALWTLDGLDSL
                     DPAMVMSALDDKSRDVRANAVRLSERFLAESNAPVQAALLKHVDDGDWLVREQLAASL
                     GALPPGARETALTLLLERHADDAVLVDAALSGLRGSEAAVLANLLKSTEPSPARETAI
                     TMLTATLVRGAQDAAVQTIMQHVSETTRPAWQRAAMMRGAEVALLGAAAPGSPAGRGG
                     RGGLAAAGRAGDPTAPGGRAGPGGAPAFPREGAAGRAGVVTDDAAAAAGARGGRGGRG
                     GGANVLRLNREPAELSALAAAGGDLGPRAAALLARIEWPGKPGASAPVTPLTAEEQQR
                     FTAGQTVYQSICLPCHQEDGRGKEAIAPSLVASAFATGAPSIPVRILLNGKEGQVGLM
                     PPLGQAFTDDQIANVLTYVRRQWGNTGAPVDVQVVKDTRAAVAGRTRPWTNAELTALS
                     GNGSGAR"
BASE COUNT          561 a         1196 c         1040 g          667 t
ORIGIN      
        1 cagtccttgc agtactgcag cgccttggca atctcagtca tgcgatcgga cccggcgggt
       61 accggatact cgaactcgat cgtggcctgg aacttccact tgttgtcgcg catcaactgc
      121 agcacttcct tgatcggggt gtcgccctgg ccaaatggca cgttcggccc gttgttcaac
      181 ttgcgatcct tgacgtgcac gtgcgtgatg cgatcgtggt actgcttgat aaacggaacc
      241 ggggaggtgt tgttgccggc gacgaaatgt ccaagatcga cgttcgccgc gttgtacttg
      301 gcctcggcaa acgtcgcctc ccagatcgcc ggcgtcaccg cggtgtggcc gtgataacca
      361 accggcatct tgtgcttgtc ggcgaattgt ccaagcctct tcgtttgtcc gactgaaatt
      421 tcgctggaca atgcgcgcgc accgagcgcc ttcgatacct ggaacgcgta gtcgagttcg
      481 tcgtccgaga acccgaagat gccgtcccac ttgacgattt caatcagtac gccggcatcc
      541 tcgtacttct tccggaattc cttgaccttg tcgagcgaca cgccagcgcg ccatttacga
      601 gtctcttcgg cggcggcctt ctgcgcggcc tcctgctcgg gcgtcagcgg tccgcggcca
      661 cggccgcggc cttcggctcc ggcggcaccc tgacctgcac cagcacctcg ttcggccgca
      721 ccaccgccag cggcggccgc ccctgcggcg gccccggcac cggctcctgc cccagctcct
      781 gcacccgtac cagcgcccgc accggccgcg gcgcccacgc ctgcactggc atcaccagct
      841 gcggctgcgc cacgtcggcc accccgtccg cgtccctgat ttgcagcgcc tgcgatcgcc
      901 gcaggcgagc cgaggaacaa ctcgacgggc tgcgctcgca attcgacggc gctgacgccc
      961 aactggacgc atcgctgaag gatctcgtca ccgctcgtgt agttgccggt gccgaagtta
     1021 taggggacgt tcatccccac ctgcacgccg gcccatttcg aattcggctt gtcggcggcg
     1081 gccaggatcg ccgaacccgg tttcaccaac agcgttgccg gcacgcccgc cagcgcgagc
     1141 cttccgaact cccgtcgcga aaacttcatg atctgctcct cactaatgct gccgacacaa
     1201 tatcgcgatt gtggtcgaac taccgcgcgc cggatccgtt gccgctcagc gcggtgagtt
     1261 ccgcgttggt ccagggacgc gtccggccgg cgaccgccgc gcgtgtgtcc ttgacgacct
     1321 gaacgtccac tggtgcgcct gtattgcccc actgccgtcg cacatacgtc agcacgttgg
     1381 cgatctgatc gtcagtgaac gcctgaccga gcggtggcat caatccgacc tgcccttcct
     1441 tgccattgag caggatgcgt acaggaattg aaggagctcc ggtcgcgaac gcggaggcca
     1501 ccagactcgg cgcgattgct tcctttccac gtccatcttc ctgatgacac ggcaggcaga
     1561 tgctctggta gacggtctgt ccagcggtga accgctgctg ctcttctgcg gtgagcggcg
     1621 tcaccggtgc cgacgcaccg ggcttacctg gccattcgat gcgcgccaac aatgctgccg
     1681 cccgcgggcc gagatcgcca cccgcggcgg caagcgcact caattcagcc ggctcgcgat
     1741 tgagccgcag cacgttcgca ccgccacccc gtccgccccg gcctccgcgt gcccccgcag
     1801 cagcagccgc gtcgtcggtc acaacgcccg cgcgtccggc ggctccctca cgcggaaacg
     1861 ccggcgcacc accgggcccg gcgcgaccgc cgggcgcagt cggatctccc gccctgccgg
     1921 cagcagccag gccgccgcgc ccaccgcggc cggctggtga acctggcgcc gcggcaccaa
     1981 gcagtgcgac ctccgcaccg cgcatcatcg ccgcacgctg ccacgcgggg cgcgttgttt
     2041 ccgacacgtg ctgcatgatc gtttgcaccg cggcatcctg tgcaccgcgc accagcgtcg
     2101 ccgtcagcat ggtaatcgcg gtttctcgcg ccggcgacgg ctcggttgac ttgagcaggt
     2161 tggcaagcac tgccgcctcg cttccgcgca gtccgctgag cgccgcatcc acgagcacgg
     2221 catcgtctgc atgacgttcg agcagcaacg tcaacgccgt ttcgcgcgcc ccaggaggca
     2281 gcgctccgag cgacgcggca agctgctcgc gcaccagcca gtcgccatcg tcaacatgct
     2341 tcaacaacgc ggcttgtacg ggcgcgttcg attcggcaag gaaccgctcg gatagtcgca
     2401 ccgcattggc acggacgtct cgcgacttgt cgtcgagtgc actcatcacc atcgccgggt
     2461 ccagactgtc gagaccgtcg agcgtccaca gcgcatgaac ccgtgtccgc ggttcggtgg
     2521 atgaagcggc gagcttcttg aggaccggca cgacgcttct gtcgccgcgc tggatcaata
     2581 gctgctgcgc ttcatctctc caccagccat tcgggtgact gagccggcca accagctgtg
     2641 tggccgattc ggatccaagt gctggcggtc cgtcgcgccg cgtcgtgtcg tgcacgattc
     2701 gccagatgcg tcccatgtgg atcggcgtct cgagcttgtg cgcgacgatg tggtcgcgca
     2761 agtactcggt gatgaaaccc ttctcctgaa tgatgccgtg gtagatgtca acgacgtaga
     2821 tggtgccgtc gggcgccgac gacaaataca cggggcggaa tcgctcgtcg gtcgccgcca
     2881 tgaactcggc attcgcgtac gctttcttgc cgcgcaatgt ggtcccgtcg tcgctgacga
     2941 tgatccggct gatcaggttc ccggtcggct ccgcaatgaa cacgtttcct gcgagctccg
     3001 ccggcagacg atcgccgcga tagaccatcg gcgagttgac gcccgtatag gtggcgaggg
     3061 ttccatcgcc acgtagctgt ccggtctggt agccgcgatt cacgcccttg ttgggacgga
     3121 ccggatatgt tgcgttcaac tcgtcgggat cgcccatgaa ctcgtagctg ccgcgggtgc
     3181 gaagaagatt cggattccgc agaaagtacg gtgtcgaaag aagatcgatg tggagcgctg
     3241 aactgttcga gttgcgataa acgcgaccga aatcgtcctg cgtcgctccc cactggccac
     3301 gcgacaacgt ttttcgcgtt tccatctttc cgtctttgta gcggaaatag gtatcccctt
     3361 cggacgtgtg catccagttg tccatcgccc acaggaggct gttctcgttg tgttcgacgt
     3421 tggcgagctc ggtaccgtag cagtcgcaca cgagatcctt cttg
//
LOCUS       DATIMH010001105         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2574413, whole genome shotgun sequence.
ACCESSION   DATIMH010001105 DATIMH010000000
VERSION     DATIMH010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574413"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VLI90_19110"
     CDS             complement(<1..1226)
                     /locus_tag="VLI90_19110"
                     /inference="COORDINATES: protein motif:HMM:NF017039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16381.1"
                     /translation="MHDAAATTPTPQAPAAPPAPLSPAMRQYRQFKSQHPGYVLFFRM
                     GDFYEMFWEDAQLAAKTLGVALTSRNKGSSDEIPMAGVPFHAVEGYLRKMIAAGHKVA
                     ICEQTEDAALAKGLVKREVVRLMTPGTLTDDPLLDGRSDNFLAAVAFHVTKADGYRAA
                     LAWVEMSTGTCVAMSGDESQVMDEIARLRPAEILIPEHASGQPHEIGNRLASLGKLAV
                     TPRPGWQFTAHHAIEQFQRQWQTRTAGGFGFADDDPAVFATAAILSYLEETQKTGLSH
                     IRPLRRHVVEDHLSIDPASWRSLEIDRTMRAGGTEGSLLSAIDRTCTPMGARLLRQWL
                     RYPLCDIEHITARQSAIAALLESPAALREIVDQLDDVCDIERIVGRIAVGRVGPRDLA
                     ALGRCLDALPAFMDRLS"
     gene            1362..>2353
                     /locus_tag="VLI90_19115"
     CDS             1362..>2353
                     /locus_tag="VLI90_19115"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSV16382.1"
                     /translation="MNRTLIILCSLVVFAAGASRAETITVATYNVQLFDRHFLAHHLT
                     GRGMPDAMSKELRQEPHVKELIDDERETNDRLNWIVASVIQDRHFNPDIICLEECCDQ
                     TDLTYFNHRWLDDGYATAIVFPTNTTRQQNVAMMLKRGFTVEEKRDQYYLEKDPVPNA
                     RGDRLFARGPSFLKVKSPGGYEFWVGVTHQKSKRVDPPLNAVGVPVKIAPDEREKLEI
                     EAGKWRLREAARTHQIMMDLAAAGPKDVMLLGDMNDELGADDIERKAGGDAVAMLVGP
                     PEDGYVLATKPLADADQFSFGGYWQTRFRSLIDHIIVSPSMKGEIETVAIFKDGL"
BASE COUNT          445 a          762 c          747 g          399 t
ORIGIN      
        1 gacaggcgat ccataaaagc cggcagcgcg tcgaggcatc gccccagtgc cgccagatcg
       61 cgcggcccca cccgccccac cgcgatcctg ccgacgatcc gctcgatatc gcaaacgtca
      121 tccagctgat cgacgatctc ccgcagggcc gccggcgatt ccagcagcgc cgcgatcgcc
      181 gactgccggg cggtgatgtg ctcgatgtcg cacagcggat agcgcagcca ctgccttagc
      241 aaccgcgcgc ccatcggcgt gcaggtgcga tcgatcgccg acagcagtga accctcggtg
      301 ccgccggcgc gcatcgtgcg gtcgatctcc aaactgcgcc agctggccgg gtcgatgctg
      361 agatgatctt cgacgacatg ccgccgcagc gggcggatgt gcgacaggcc ggtcttctgc
      421 gtctcttcca ggtagctgag gatcgccgcc gtggcgaaga cggcggggtc gtcgtcggcg
      481 aagccgaatc caccggcggt gcgcgtctgc cactggcgtt gaaattgctc gatcgcatga
      541 tgcgccgtga actgccaacc cggcctgggt gtgacggcta acttaccgag cgacgcgagc
      601 cgattgccga tctcgtgcgg ctggcccgac gcgtgttccg gaatgagaat ttccgccggc
      661 cgcaggcggg cgatctcgtc catcacctgc gattcatcgc cgctcatcgc gacgcacgtt
      721 ccggtggaca tctcgaccca cgccagcgcc gcgcgataac catccgcctt ggtcacatga
      781 aacgcgactg ccgccaggaa gttgtcgctc cgcccatcga gcagcggatc atccgtcaac
      841 gtccccggcg tcatcaggcg gacgacctcg cgcttcacca accccttggc cagcgcggca
      901 tcttcggtct gctcgcagat cgccaccttg tgccccgcgg cgatcatctt ccgcaggtaa
      961 ccttcgaccg catgaaatgg cacgcccgcc atcggaattt catcggacga gcccttgttc
     1021 cggctcgtca gcgcgacgcc cagcgtcttg gcggccagct gcgcgtcttc ccagaacatc
     1081 tcgtaaaaat cgcccatgcg gaagaacagg acgtaaccgg gatgttgcga tttgaattgc
     1141 cgatactgcc gcatcgccgg tgaaagcggc gcgggtggcg cggctggcgc ttgaggcgtc
     1201 ggagtggtcg cggcggcgtc gtgcatgggt gtgcgcccga aggtatgtca catccgagcc
     1261 ggatacaata cattcgcgcc aaagcccacg gctctgccgt gggacgcgtg ttttcaaatc
     1321 cggtcccgcg gcagagccgt gggcttttct tcgggagcat cgtgaatcga acgctcatca
     1381 ttctttgctc gctcgtcgtc ttcgccgccg gcgcatctcg cgctgagacg atcaccgtcg
     1441 cgacctacaa cgtgcagttg ttcgaccggc actttttggc gcaccacctg accggccggg
     1501 ggatgccgga tgcgatgtcg aaggagcttc ggcaggagcc gcacgtgaag gaattgatcg
     1561 acgacgagcg cgagacgaac gatcggctga actggatcgt cgccagcgta atccaggacc
     1621 ggcacttcaa cccggacatc atctgcctgg aagaatgctg tgatcagacg gacctgacgt
     1681 acttcaacca tcgctggctg gatgatggct acgcaaccgc catcgtcttt cccaccaaca
     1741 cgacgcggca gcagaacgtg gcgatgatgc tgaagcgcgg cttcaccgtc gaagaaaaac
     1801 gcgaccagta ttacctggaa aaagacccgg tgccgaacgc gcgcggcgac cggctgttcg
     1861 cgcgcgggcc gtcattcctg aaagtcaaat caccaggcgg atacgaattc tgggttggcg
     1921 tcacgcacca gaagagcaag cgggtggacc cgccgctgaa cgcggtcggc gtgccggtga
     1981 agatcgcgcc cgacgagcgc gagaagctgg aaatcgaagc cggcaaatgg cggctgcgcg
     2041 aggcggcgcg gacacaccag atcatgatgg acctggccgc cgcggggccg aaggatgtga
     2101 tgctgctggg cgacatgaac gacgagctgg gcgcggacga catcgaacgg aaagccggcg
     2161 gcgacgccgt cgccatgctg gtcggtccac cggaagacgg ttacgtgctg gcgaccaaac
     2221 cgctggcgga cgcggatcaa ttctccttcg gcgggtattg gcagacccgc tttcgttcgc
     2281 tgatcgatca catcatcgtg agcccgtcga tgaagggcga gatcgagacg gtggcgatct
     2341 tcaaagacgg cct
//
LOCUS       DATIMH010001106         5601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3439963, whole genome shotgun sequence.
ACCESSION   DATIMH010001106 DATIMH010000000
VERSION     DATIMH010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3439963"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VLI90_19120"
     CDS             complement(<1..198)
                     /locus_tag="VLI90_19120"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HSV16383.1"
                     /translation="MPANRRAFATLALGALGVVFGDIGTSPLYTIQSTFGDAHAAIAP
                     NAENILGVLSVMFWLLVLIVSL"
     gene            complement(281..853)
                     /gene="kdpC"
                     /locus_tag="VLI90_19125"
     CDS             complement(281..853)
                     /gene="kdpC"
                     /locus_tag="VLI90_19125"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HSV16384.1"
                     /translation="MGKLVRQSFAMLLAMTVLTGAVYPLLVTAAAQLLFHDAANGSVI
                     ERGGKPLGSALIGQPFTDPKYFWARPSATTPFMDNSAASGASNLGPTNPALIDAVKQR
                     VDALRAADPDNAAPVPVDLVTASGSGLDPHISPAAAAYQVARVARVRGIAEADVARLV
                     TIATDGRTFGILGEPRVNVLELNLRLDGAW"
     gene            complement(880..1704)
                     /locus_tag="VLI90_19130"
     CDS             complement(880..1704)
                     /locus_tag="VLI90_19130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HSV16385.1"
                     /translation="MQIMRFQLACLVLLTAAIQARAQDAAIAPASAYSLSGNVALTSD
                     YLFRGLTQTWGSPAIQGGGDLTLANGFAAGLWASSVSQKTYPGAALELDLYASYGSSF
                     NEDWSWRAGLYRYIYPNGNLDAAGLPPRSFDTLEANAALTWKWLTLKYSHALTDYFGI
                     DTEQGYRSDSKGTGYVQLDAAVPVGESWSVALHAAHTDIPTSLATPLANGASNPSYSD
                     FGATLKWQFATHWNASVGMTYANNDAFYSRTADLSNVENTRDVGGTRGFVMLQGTL"
     gene            complement(1718..3817)
                     /gene="kdpB"
                     /locus_tag="VLI90_19135"
     CDS             complement(1718..3817)
                     /gene="kdpB"
                     /locus_tag="VLI90_19135"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HSV16386.1"
                     /translation="MTPHPQTSAMSRELIMPAVADAFRKLSPRAQFRNPVMFVVFVCS
                     VMTTGLWAQAIAGHGEAPTAFIFWVAVWLWFTLLFANFAEAIAEGRGKAQAANLRGAR
                     RDVVANRIASLDTRESVCVGAAHDLRKGDLVVVKAGEFIPGDGDVVDGAATVDESAIT
                     GESAPVIRESGGDRSSVTGGTRVLSDWIIVRISCNPGESFLDRMIAMVEGASRRKTPN
                     EIALTILLAKFTLIFLLACATLLPYSLYAVTSSGHGEPITITVLVALLVCLIPTTIGG
                     LLSAIGIAGMDRMIQANVIAMSGRAVEAAGDVDVLLLDKTGTITLGNRQAVAFHPAPG
                     IELPALAGAAQLASLADETPEGRSIVVLAKTSFGLRECALSQVTSRFVPFSANTRMSG
                     VDLKADCGESGLASGRAIRKGALDAIEKYLTAMNSAVPIEVRRAAEAIARKGGTPLVV
                     CENARALGVIELKDIVKSGIRERFLELRAMGIRTVMITGDNPLTAATIAAEAGVDDFL
                     AEATPEAKLKLIRDIQAEGRLVAMCGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMV
                     DLDSNPTKLIEVVEIGKQMLITRGALTTFSIANDVAKYFAIIPAAFAATYPTLNALNV
                     MHLATPQSAILSAVIFNALIIIFLIPLALKGVRYRALGAGALLQRNLLVYGIGGLIVP
                     FVGIKVIDLMLVALHAA"
     gene            complement(3979..>5601)
                     /gene="kdpA"
                     /locus_tag="VLI90_19140"
     CDS             complement(3979..>5601)
                     /gene="kdpA"
                     /locus_tag="VLI90_19140"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HSV16387.1"
                     /translation="SWQRYAMVMLMFNVAGLLFVYLLQRIQQWLPLNTQAMPAISADS
                     AMNTAISFATNTNWQGYGGESTMSYLTQMLGLAVQNFLSAATGIAVLVALIRGFIRRN
                     ASGLGSFWVDMTRTTLYVLLPLSFVLALLLSAQGVPQNLTPYATVPLLEATTSTEPLK
                     EADGSTVKDAEGKDVVKETRVTEQSLPLGPVASQIAIKQLGTNGGGYYNANSAHPYEN
                     PTPLTNFLELLAILLIPAALCYTFGKMVGDARQGWALLATMLIIFVPLLLACTAAEQA
                     GNPAMSSLGVDQSVSALQAGGNMEGKETRFGIVNSTLWATATSAASNGSVNAMLDSFT
                     PLGGLVPMWLIQLGEVIFGGVGSGLYGMLAFAIVAVFIAGLMVGRTPEYLGKKIEAYE
                     MKMASLVVLIPCALVVIGTAVAVMAPAGLAGMANPGIHGFSEVLYGLSSASNNNGSAF
                     GGLSANTPFYNTLLGICMFFARYPLAIGMLAIAGSLAAKKHVPPSAGTLPTHTPLFIA
                     LLASTVIVIGALTFLPALALGPIAEQLSALGH"
BASE COUNT         1047 a         1740 c         1781 g         1033 t
ORIGIN      
        1 caacgacacg atcaacacca gcaaccagaa catgaccgag agcacaccaa gaatgttttc
       61 ggcattcggc gcgatcgcgg catgcgcgtc gccgaacgtg ctctgtatcg tatacaacgg
      121 actggtgccg atatcgccga acacgacacc gagcgcccct agcgcgagcg tcgcgaacgc
      181 gcgccggttg gccggcatgg aattgattgc taacgtcatg atgcattccc ctgttgcgct
      241 tgcaatgacc cgctaataac cgagggcccg atggctgcgc ctaccacgcg ccatcgaggc
      301 ggagattcaa ttcgagcaca ttgacgcgcg gttcgccgag aatcccgaaa gttcggccgt
      361 cggtggcgat cgtcaccaac ctggccacgt cggcttcggc tattccgcgc acgcgtgcaa
      421 cgcgcgcgac ctggtaggcg gcggctgccg gcgatatgtg gggatcaaga ccgctgcccg
      481 aggcggtgac cagatcgacc ggcaccgggg cggcgttgtc cggatcggca gcacgcagcg
      541 catcgacacg ttgcttgact gcatcgatca gggctggatt ggtcggtccg aggttggacg
      601 cgccggatgc cgcggaattg tccatgaatg gcgtggtcgc cgatgggcgg gcccagaaat
      661 atttgggatc ggtgaagggc tgtccgatca acgctgatcc gagcggtttg cctccgcgct
      721 cgatgacact gccgttcgcc gcgtcatgga acagcagctg cgccgcagcg gtgaccagca
      781 atggatagac ggcgccggtg aggacggtca tcgcaagcag cattgcgaat gattgacgaa
      841 caagcttgcc catgaatcaa ctccatttct cttgttgcgc tacaacgtgc cctgcagcat
      901 cacaaagccg cgcgtgccgc cgacatcgcg cgtgttctcg acgttgctga gatcggccgt
      961 gcggctgtag aacgcatcgt tgttcgcata ggtcatgccg acgctggcat tccagtgcgt
     1021 ggcgaactgc cacttcaggg tcgcgccaaa gtcgctatag ctcgggttgg aagcaccgtt
     1081 tgccagcggc gtggcaagcg atgtcggtat gtcggtatgg gcggcatgca gggcgacgct
     1141 ccacgattcg ccgaccggaa ctgctgcatc gagctgcacg taaccggtgc ccttggaatc
     1201 gctgcgataa ccctgttcgg tgtcgatgcc gaagtagtcg gtcagcgcgt gcgaatattt
     1261 cagggtcagc catttccagg tcagcgcggc gttcgcttcg agcgtatcga atgaacgcgg
     1321 gggcaggccg gcggcgtcga gattcccgtt cgggtagatg tagcggtaga ggcccgctcg
     1381 ccacgaccaa tcctcgttga acgaactgcc gtagctcgcg tacaggtcga gttcgagcgc
     1441 ggcgccgggg taggtcttct ggctcacgct cgatgcccaa agccctgcgg caaacccgtt
     1501 cgccagcgtc agatcgccgc cgccctggat cgcggggctg ccccaggtct gggtcaggcc
     1561 gcgaaacagg tagtcggatg tcagtgcgac gttcccggac aagctgtaag ccgatgccgg
     1621 tgcgatagcg gcgtcctgcg cccgcgcctg aattgcggcg gtgagcagga ccaggcacgc
     1681 caattggaaa cgcatgatct gcatggtcgg atgactctca ggcagcgtgc agcgcgacga
     1741 gcatcaggtc gatgaccttg atgcccacga acggcacgat gagtccgccg atgccgtaga
     1801 ccagcagatt gcgctgcaac agcgcgccgg caccgagcgc ccggtagcgc acgcccttga
     1861 gcgcgagcgg gatcaggaaa ataatgatca gcgcgttgaa gatcacggcc gacaggatcg
     1921 cgctctgcgg tgtggccagg tgcatcacgt tgagcgcgtt gagtgtggga taggtcgccg
     1981 caaatgcggc cggaatgatc gcgaagtatt tggcgacgtc gttcgcgatc gagaaggtgg
     2041 tcagcgcacc gcgcgtgatc agcatctgct tgccgatctc gaccacttcg atcagcttgg
     2101 tcggattgga atccaggtcg accatgttgc cggcttcctt cgctgcctgg gttccgctgt
     2161 tcatcgcgac cgccacgtcc gcctgggcga gcgcgggcgc gtcgttggtg ccatcgccgc
     2221 acatcgcgac gaggcgtccc tcggcctgga tgtcgcggat cagtttgagc ttggcttcag
     2281 gcgttgcctc ggcgaggaag tcgtcgacac ccgcttcggc cgcgatcgtt gcggcagtca
     2341 gcgggttgtc gccggtgatc atgaccgtgc ggatacccat cgcgcgcaac tcgaggaagc
     2401 gttcccgaat gccgctcttg acgatgtcct tcagttcgat cacgccaagc gcccgggcgt
     2461 tttcacagac gacgagcggt gtcccgccct tgcgggcgat ggcttccgcg gctcgccgca
     2521 cctcgatcgg cacggccgaa ttcatcgctg tcaaatattt ttcgatcgcg tcgagcgctc
     2581 ccttgcggat cgcgcgcccg cttgccaggc cgctctcgcc gcagtcagcc ttcagatcca
     2641 cgccgctcat gcgcgtgttg gccgagaacg gcacgaaacg gctggtcact tgactgagcg
     2701 cgcactcgcg caggccgaat gatgttttcg cgagcacgac gatgctgcgc ccttccggtg
     2761 tttcgtcggc cagtgaagcc agttgcgcgg ctccggccag cgccggcaac tcgatccccg
     2821 gagcagggtg gaacgcgacc gcctggcggt tgccgagcgt gatcgttccg gtcttgtcga
     2881 gcagcagcac gtcgacatcg cccgccgcct cgaccgcgcg accggacatt gcgatcacgt
     2941 tggcctggat catccggtcc atgccggcga ttccgatcgc agacagaagg ccgccgatcg
     3001 tggttggaat caggcagacc agcaaggcga ccagcaccgt aatcgtgatc ggttcgccgt
     3061 ggccggatga agtgaccgcg tagagcgagt agggcagcag cgtggcgcag gccagcagga
     3121 agatcagcgt gaacttcgcg agcaggatcg tcaacgcgat ctcgttaggc gtcttgcgcc
     3181 gcgatgctcc ttcgaccatc gcgatcatgc ggtcgaggaa gctctcgccc ggattgcagc
     3241 tgatgcgcac gatgatccag tccgaaagca cgcgcgtgcc gcccgtgacc gaactgcggt
     3301 cgccaccgga ctcccggatc accggcgccg attcaccggt gatcgcgctc tcgtcgaccg
     3361 tggccgcgcc atcgaccacg tcgccgtccc ctggaatgaa ttcgccggcc ttgacgacga
     3421 cgagatcgcc cttgcgcaga tcatgcgccg cgccgacaca gaccgactcg cgggtgtcga
     3481 gtgatgcaat ccggttcgcc acgacatcgc gccgtgcacc gcgcaagttg gcggcttggg
     3541 ccttgccgcg gccttcggcg attgcttcgg cgaagttggc gaacagcagg gtgaaccaga
     3601 gccagaccgc gacccagaaa ataaatgccg tcggcgcttc gccatgaccc gcaatcgcct
     3661 gcgcccacag tccggtcgtc atgacgctgc agacgaagac tacgaacatc accggattgc
     3721 gaaactgcgc gcgcggcgac agcttgcgga acgcatcggc gacagccggc atgatcaact
     3781 cgcggctcat tgcgctggtt tgcggatggg gcgtcatgat caaaaatccc agaacttcca
     3841 aaacattgaa ccacggagaa cacggagagg gcttctagtg atgcaaatga aaagggatct
     3901 cgctttgttt cttggcgctg atcttctgct gctctccgtg ttctccgtgt tctccgtggt
     3961 tcaggctttc gctctgactc aatggcccag agcggagagt tgttcggcga tcggtccgag
     4021 cgccagcgct ggcagaaacg tcagcgcgcc tatcacgatg accgtgctgg cgagcaatgc
     4081 gatgaacagc ggcgtgtgcg tcggcaacgt gccggccgag ggtggaacgt gttttttcgc
     4141 cgccagggat cctgcaatcg cgagcattcc aatcgcgagc ggataacgcg cgaagaacat
     4201 gcagatgccg agcagcgtgt tgtagaacgg cgtgttcgcg gacaggccgc caaaggcgct
     4261 gccgttgttg ttcgatgccg acgacagccc atacagcact tcgctgaagc cgtgaatgcc
     4321 ggggttcgcc attccggcca ggccggcagg cgccatcacg gcgactgcgg tgccgatcac
     4381 gaccagcgcg catggaatca gcacgaccag gctcgccatt ttcatttcgt aggcttcgat
     4441 ctttttgccg aggtattcgg gcgtgcgccc gaccatcaac ccggcaatga acacggcgac
     4501 gatcgcgaac gccagcatgc cgtacagtcc ggagccgacc ccgccgaaga tcacctcgcc
     4561 gagttgtatc agccacatcg gcaccaggcc gcccagcggc gtgaacgaat cgagcatcgc
     4621 gttgaccgac ccgttcgagg cagcgctcgt cgccgttgcc cagagcgtcg aattgacaat
     4681 gccgaagcgc gtttccttgc cttccatgtt gccgccggcc tgcaaggccg acacggactg
     4741 gtcgactcca agagaactca tcgccgggtt gcccgcttgc tccgcggcgg tgcaggccag
     4801 cagcagcggt acgaaaatga tcagcatcgt cgcgagcagg gcccagccct ggcgcgcgtc
     4861 accgaccatt tttccaaatg tgtaacacag tgcggccgga atcagcagga tcgccagcag
     4921 ttcgaggaaa ttcgtcagcg gtgtcgggtt ctcgtacgga tgcgcggagt tggcgttgta
     4981 gtagccgccg ccattcgtgc cgagctgctt gatcgcgatc tgcgatgcga ccgggcccaa
     5041 tggaagcgat tgctcggtta cccttgtttc ctttacgacg tccttgcctt cggcgtcctt
     5101 cacggtgctg ccatccgcct ccttgagggg ttcggtactg gtggtcgcct ccagcagcgg
     5161 caccgtcgcg taaggcgtca ggttctgggg cacgccctgg gccgacaaca gcaacgcaag
     5221 gacgaaggac aagggcagca gcacataaag tgtcgtccgc gtcatgtcga cccagaagct
     5281 gccgaggccg gatgcgttgc gccggatgaa accgcggatc agcgcgacca acacggcgat
     5341 tccggtggcc gcggacagga aattctgtac ggccaggccg agcatctgcg tcagatagct
     5401 catcgtggat tcaccgccat agccttgcca gttcgtgttc gtggcaaagc tgattgcagt
     5461 attcatcgcc gaatcggcac tgatcgcagg catggcctgt gtattgagcg gcagccactg
     5521 ctggattcgc tggagcaggt acacgaacaa cagcccggcc acgttgaaca tcagcatgac
     5581 catggcgtag cgctgccagc t
//
LOCUS       DATIMH010001107         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2002942, whole genome shotgun sequence.
ACCESSION   DATIMH010001107 DATIMH010000000
VERSION     DATIMH010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002942"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /locus_tag="VLI90_19145"
     CDS             <1..1691
                     /locus_tag="VLI90_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16388.1"
                     /translation="AVQPATAPGFRHFLHTPLPSQRFRAFGVDPLESPALQPRVRGLM
                     HRLSRRMDAEICWPRRDDEAVEQWENPRIPSGYTYLLQFVAHDLVHSAIPLSVAGTLG
                     AGTANARRMPLRLETLFGSGPVGSPHIYALDAPNDDRRTKLRLGRMRWKENEPATGCP
                     FRDIARTGAENVTGIDRSIAGMRVALTEALVADPRNDDHAVMSQLTALFALLHNSLID
                     HVRRQERSHGRNDRFGAAYKRFLCVREAVTLIYHNVLRKDLLRRLIHPTIYAAYSGPN
                     PNFIDRPDQSVGDFAAGEWQIPFEFSHGAFRFGHAMVRPEYNINDLSNQDLYNTLEKT
                     SANDPVNMPLDESWMIRWSHFFRINGSKPNFSRRIGPYLSDGLGNDQIFPAFDETERV
                     GLLYRDLLGAGLAGMWSLDALIAEIAARRPHFIAMSRLLFDRDYRISQLRAWLVATPA
                     YGALADEDIETLSNDPPLPFFIPFEAMRQPGAEGLHLGPLGSIMVSEVIFGALARDLR
                     SASGDPLTDQLAGLSREHYPANFFQDIPDISSMAQLVEFTAEIAELKQAVPAFL"
BASE COUNT          364 a          607 c          556 g          348 t
ORIGIN      
        1 acgctgtgca gccggcaaca gccccgggct ttcgccattt cctgcatacg ccactgcctt
       61 cacagcggtt tcgcgctttc ggtgtcgacc cgctggaatc tcctgcactc cagccaagag
      121 tgcgcggact gatgcaccgc ctgtcgcggc ggatggatgc cgaaatatgc tggccgcggc
      181 gcgacgacga agcggtcgaa caatgggaaa accctcgcat cccttcgggt tatacctact
      241 tgctgcaatt cgttgcgcac gacctcgtgc actcggcgat cccgctttcg gtggccggaa
      301 cgctcggcgc cggcacagcc aatgcgcgcc gcatgccgct tcggctcgaa accctgttcg
      361 gcagcggtcc ggtcggatcg ccgcatattt atgcactcga cgcgccgaac gacgatagac
      421 gcaccaagct gcggcttggg cggatgcgct ggaaagagaa cgagcccgcc accggctgcc
      481 cgttccgcga tatcgcgcga accggggccg agaacgtcac cgggatcgac cgcagcattg
      541 caggtatgcg ggttgccctc accgaggcgc tggtcgccga tccgcgcaac gacgaccacg
      601 ccgtgatgtc gcagctgacg gcgctgttcg cgctgcttca caacagcctg atcgatcacg
      661 tccgccgcca ggaacgaagc catgggcgga acgaccgctt cggcgccgcc tacaagcgct
      721 tcctgtgcgt tcgggaggcc gtgacgctga tctatcacaa cgttctgcgc aaggatctgt
      781 tgcggaggct gatccatccg acgatctatg cggcctatag tggaccaaat ccgaacttca
      841 tcgatcggcc cgaccaatcc gtcggggatt tcgcggccgg cgaatggcaa attccgttcg
      901 agttctcgca cggcgcgttt cggttcggcc acgccatggt gcgacccgaa tacaacatca
      961 acgacctgtc gaaccaggac ctctacaaca ccctggaaaa gacctccgcg aacgatccgg
     1021 tcaacatgcc gctcgacgag agctggatga tacggtggtc gcatttcttc cggatcaacg
     1081 gatcgaaacc gaatttcagc cggcgcatcg gaccctatct cagcgacgga ttgggcaacg
     1141 accagatctt cccggcgttc gacgagaccg aaagggtcgg gttgctgtat cgcgacctgc
     1201 tcggcgcggg cctcgccggc atgtggtcgc tggacgcgct gatcgcggag atcgccgcgc
     1261 gcaggccgca cttcatcgcg atgtcgcgcc tgctgtttga tcgcgactac cgcatcagcc
     1321 agctacgcgc gtggctcgtc gccacgccgg cttatggcgc gctggcggac gaagacattg
     1381 aaacactttc gaacgatcct ccgctgccgt tcttcatccc gttcgaagcc atgcggcagc
     1441 ccggcgcgga agggctgcac cttgggccac tcggctcaat catggtgtcg gaagtgattt
     1501 tcggcgcgct cgcccgcgat ctgcgctctg ccagcggcga tccgctgacc gatcagctgg
     1561 ctggtttgtc ccgtgaacat tatccggcaa attttttcca ggatattccc gatatttcga
     1621 gcatggcgca gctggtggaa ttcaccgctg aaatcgccga gctgaagcag gccgtgcccg
     1681 cattcctgtg agcagaataa tctgtctcac tcaacccgga ggagtagaac catgcagcgc
     1741 atcgaaaggc tacaggtgac caaccacgag cgttggggca agctggtgaa gacctgggcg
     1801 accggccaga actatcttga cgacgacaac acctatccgc tgccggagac catggacgag
     1861 ttcaaggaac agctc
//
LOCUS       DATIMH010001108         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1131978, whole genome shotgun sequence.
ACCESSION   DATIMH010001108 DATIMH010000000
VERSION     DATIMH010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131978"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2660)
                     /locus_tag="VLI90_19150"
     CDS             complement(<1..2660)
                     /locus_tag="VLI90_19150"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HSV16389.1"
                     /translation="MSKELTARKSTKSPRPTTLPARVEPMETRLLFASISVTNMGPAH
                     DNVGDSTTGWTGFLLTLHADPGDLISAVDYGQTSTSTRGIFGQLLQDWSPSKGGPLAS
                     PVGATANDTNGGTNGTDSHILLLNRGPTVADAFEDSDVTQPAGAPTSGAQDIWGTGTY
                     MHGVFGIPGASQANVLPFAYVVLNDSSIGSYEVQMAEAPTNGNQSVVTVTGQFGPPAL
                     TVSASAVEEPNVGGTVTETFTATLSDAPVSSPSTVDFATADGTAIAGTDYDPVSGTLT
                     FNPGETQKTIDVTVHGVAGTATAKTFTLNFTNAHKLIPPQPVTATITPPPTVTATPSP
                     LTEPDPGTDAVEAVTLTLSAAPLHTATIDVATADGTAIAGTDYDALPTTTVTFSPGQT
                     TQTVNITVHGVAGTAATKSFALNFSNPNGLIAPASPAMVTIAPQPSLSATTTPVLEPL
                     VGSTAQAIFTLTLSQASTQAAVVDVATADGTAIAGSDYDAVPTTTVTFAPGETSKQVT
                     VTVHGVVGTSADKTFSLNLSNGHGLAIAVPSVTETITAQIPIAFDAKKPLKYTDTAGH
                     NVFVQLIGPGTGSAMFVAGTGDPIQVAVAGTTAASSLIVRSIGGATTLHDVVVQGPLN
                     FITGNANVVGNVSIAGTLRAAVLGNFSGQKTLAIGQGGVNTVLRFGNVSDLSLSTPGT
                     IASLVALSWTDSNATPDTITAQGGINVMNIVGPLGASLSVGSGGLGVGRVVGDVTGGT
                     WDIGGSSRAIVVKSTPSTWVANFAGDLNTFVALGNSAGSLTAHSIRVARIVGSLSGAL
                     HTTGTAAELGTAPGLGVLFVGGSINGADVRSVSDITLVSAASVVNSTVFAGVASTTVG
                     LPASAAEFTAPSSILTFVVRGGPALASAFS"
BASE COUNT          397 a          845 c          988 g          477 t
ORIGIN      
        1 ttggagaatg cgctggccag cgcggggccg ccgcggacga cgaacgtcag gatcgacgat
       61 ggcgccgtga actcggcggc gctggcgggg aggccgaccg tcgtgctggc gacgccggcg
      121 aacactgtcg agttgacgac ggacgcggcg ctgaccagcg tgatatcgct gacgctgcgg
      181 acgtcggcgc cgttgatcga tccgccgacg aacaggacgc ccaggcccgg tgcggtgccc
      241 agctcggccg cggtgccggt ggtatgcagc gcgccgctca ggctgccgac gatgcgggcg
      301 acgcggatcg aatgcgcggt gagcgagccg gcggaattgc ctagcgcgac gaacgtgttg
      361 agatcaccgg cgaagttcgc cacccaggtg gacggggtgg acttcacgac aatcgcgcgc
      421 gagctcccgc cgatgtccca cgtgccaccg gtgacgtcgc cgacgaccct gccaacgccg
      481 agcccaccgc tgccgaccga caggctcgcg ccgagcggac cgacgatgtt catcacgttg
      541 atgccgccct gtgcggtgat ggtgtcgggc gtcgcgttgg aatcggtcca gctgagcgcg
      601 accagagatg cgatcgtccc cggcgtggag agcgacagat cgctgacgtt cccgaatcgc
      661 aggacggtgt tcacaccgcc ttggccaatg gccagcgtct tctgaccgct gaagttgccg
      721 agcaccgcgg cgcgaagcgt gccggcgatg ctgacgttgc cgacgacgtt ggcgttgccg
      781 gtgatgaagt tgagcggccc ctgcacgacc acatcgtgca gcgtggtcgc gccgccgatc
      841 gatcggacga tcagcgacga cgcggccgtc gtgccggcga cggcgacctg gatcggatcg
      901 cccgtcccgg cgacgaacat cgccgatccc gtgcccggac cgatcagttg aacgaacacg
      961 ttgtgcccgg ccgtgtcggt gtacttcagc ggcttcttcg cgtcgaacgc gatcgggatc
     1021 tgcgcggtga tcgtctcggt caccgatggg actgcgatgg ccaggccatg tccgttcgac
     1081 aagttcaatg agaacgtctt atcggcactg gtgccgacga cgccatgcac ggtgacggtg
     1141 acctgcttgc tcgtttcgcc cggcgcgaag gtgacggtcg tcgtcggcac ggcgtcgtaa
     1201 tcgctgccgg cgatggcggt gccgtcggcc gtcgcgacat cgaccaccgc cgcctgcgtc
     1261 gatgcctggc tcagcgtcag cgtgaagatc gcctgcgccg tcgaaccgac gagcggttcg
     1321 agcacgggcg tcgtggtcgc gctcagcgac ggctgcggcg cgatggtgac catcgccggt
     1381 gaagccggcg cgatcaaacc gttcggattt gagaagttca gcgcgaacga cttggtggcg
     1441 gcggtgccgg cgacgccgtg gacggtgatg ttcacggtct gcgtcgtctg ccccggtgag
     1501 aaggtgacgg tggtcgtcgg cagcgcatcg taatcggtgc cggcgatggc ggtgccgtcg
     1561 gcggtcgcga cgtcgatcgt cgccgtgtgc agcggcgctg cgctgagcgt cagcgtgacc
     1621 gcctcgaccg cgtcggtgcc gggatcgggt tcggtcaacg gcgacggtgt cgccgtcacc
     1681 gtcggtggcg gcgtgatcgt ggccgtcacc ggttgcggcg ggatcagctt gtgtgcgttg
     1741 gtgaagttca gcgtgaacgt cttggcggtc gcggtgccgg ccacgccgtg cacggtcacg
     1801 tcgatcgtct tctgcgtttc gcctgggttg aaggtcagcg tgccggagac cggatcgtaa
     1861 tcggtgccgg cgatggcggt gccgtcggcc gtggcgaaat caacggtgga cggcgaggag
     1921 accggcgcat cgctcaaggt cgcggtgaac gtttcggtca ccgtgccgcc gacattcggt
     1981 tcctccaccg ccgacgcgct caccgtcagc gcgggtgggc cgaactggcc ggtcaccgtc
     2041 accaccgatt ggttgccgtt cgtcggcgcc tcggccatct gaacttcata gctgccgatc
     2101 gagctgtcgt tcagcacgac gtacgcgaac ggcaacacgt tggcctggct ggcgccggga
     2161 atgccgaaca cgccgtgcat gtaggtgccg gtgccccaga tgtcttgcgc gccgctggtg
     2221 ggggcgccgg cgggttgggt gacatcgctg tcttcaaatg cgtcggccac ggtgggcccg
     2281 cggttgagca gcaggatgtg gctgtcggtt ccgttcgtcc cgccgttggt gtcattcgcc
     2341 gtcgccccca cgggcgaagc gagcgggcca cccttgctcg gcgaccagtc ctgcagcagc
     2401 tgtccgaaga tgccgcgcgt tgaagtcgac gtctggccgt aatccaccgc cgagatgaga
     2461 tcgcccgggt cggcgtgaag cgtcagcaga aaaccggtcc agccggtggt cgagtcgccg
     2521 acgttgtcgt gtgcggggcc catgttggtc accgagatgg aagcgaacaa caggcgggtt
     2581 tccatcggtt cgacgcgtgc gggcagcgtt gtcgggcgtg gggacttggt ggacttacgg
     2641 gcagtaagct ctttcgacat cggcttctcc ggtgggttcg ggggtttcct cgtgcgcgat
     2701 gcacgcg
//
LOCUS       DATIMH010001109         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4595728, whole genome shotgun sequence.
ACCESSION   DATIMH010001109 DATIMH010000000
VERSION     DATIMH010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4595728"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..312
                     /locus_tag="VLI90_19155"
     CDS             88..312
                     /locus_tag="VLI90_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16390.1"
                     /translation="MAEQKSDRFTVDHTDQSETQLTYDPATPEEVAAALDYATGAGIA
                     FRALNAAAIAALQEAPAVASATVNFTGTEQ"
     gene            309..1250
                     /locus_tag="VLI90_19160"
     CDS             309..1250
                     /locus_tag="VLI90_19160"
                     /inference="COORDINATES: protein motif:HMM:NF039819.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HSV16391.1"
                     /translation="MTMRLTRKQLLLARLETTYGVAPALDVNQAMLVHNLSFKGAGSS
                     MLSRPRAYPDFQVSSMGVAPSPQSLSFDVEAAGSGAQGTQPLWGRFMRACGMGEFVPD
                     DSSYVDYYPLSPPFPSSISQKFNWDGTAHLLTGSRGNVTFSAEAGKEPLFSFAFTALY
                     NAPTDTALLDVTDRLNDFLDPETVSDNQTAFALFGKQAPLSALTFDLNNTSSFRDKPN
                     LASVDITDRPQPNGKATVEASQVADVDWFSLYKAKTLGALQFAHGRTKGNKIAIGSPY
                     VQLGEPNYTNVNGVLHVELPLYFIRASNDRELRIRVY"
     gene            1319..1630
                     /locus_tag="VLI90_19165"
     CDS             1319..1630
                     /locus_tag="VLI90_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16392.1"
                     /translation="MQKPLNGGGFSEELLTAKFEVISDGDYAELVGSPLSRLSTPDKP
                     QGDLPLLQRALKGFEGLSDEDHKAVPDDVAVPVLLAQPYVVVGLVRGYMEMLGKRATK
                     N"
     gene            1651..2028
                     /locus_tag="VLI90_19170"
     CDS             1651..2028
                     /locus_tag="VLI90_19170"
                     /inference="COORDINATES: protein motif:HMM:NF020388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1799 domain-containing protein"
                     /protein_id="HSV16393.1"
                     /translation="MGEYSDGGDDTFFDDLRQWGSPESKIAKLKAQRKQNAPAEFFVE
                     PENRDVVRVFMRALTQWRGQSVVAGKHLLQLRSGLVYEALPTIATALRVEMDEPLLDA
                     LRVMEAEALLVHAQRQEQLLKGR"
     gene            2077..>3592
                     /locus_tag="VLI90_19175"
     CDS             2077..>3592
                     /locus_tag="VLI90_19175"
                     /inference="COORDINATES: protein motif:HMM:NF018497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HSV16394.1"
                     /translation="MLLADASGVVGAGDQSTVAMDKVAVSAKAAGDAGTQMAAGIDAA
                     KLATDGLARSASLAATVTQAGTEAQVQQIAKLTQVSAANISATESVSALAAAQSAYNT
                     TVAQARVLLASGLLSEQGYAQAVEGATAKLALSRAMHDLSSTSLRAEAAAAAAVGAAL
                     AGFGEAEVAAGTNTAAFTELIRAASREAGVGREKFAALHASWELLADNPVAAIIAAAA
                     VATVAGVVAWDLYDTAERRVEGTAVSLGNIFNLSRQQLAAFADQIAEAADVSERSGTQ
                     MESSFLSANLSPDLWNAAVTASMHLANVWRTDVPAAAQKLAEALKDPKGAGEQLLSSL
                     NALDPQIKQLIDDLTERGNSDQAADKILERVNQQLRDMPDTSSNISRVFHQMEADLGT
                     MVSNAGKLFDTLTNFQPPAWLLFLERQANVAANLASGNLLGAGAAATVPNASTPDATV
                     SVTGEPGGAISSITSDLARRQNQLAQDSNTIDQYQQRIDSVKNSIHNLNVVLIDE"
BASE COUNT          707 a         1155 c         1132 g          598 t
ORIGIN      
        1 gcaggcggcg cggaagtcga tcaggacgcg ggcacgctgc tttatccctt caccttcggc
       61 acaggctaca ctttgaggaa aaactaaatg gctgaacaga aatctgatcg ctttacggtc
      121 gatcataccg accagagcga gacgcagctc acctacgatc ccgcgacgcc cgaagaggtt
      181 gccgccgcgc tcgactacgc gacaggcgcc ggcatcgcct tccgcgcgct caacgctgcg
      241 gcgattgctg cgctgcagga ggcgccggcc gtcgcgtctg cgaccgtcaa ctttacgggg
      301 actgaacaat gaccatgcgc ctcacacgca agcagctgct gcttgcccgg ctcgaaacca
      361 cttacggcgt cgcgccggcg ctcgacgtca accaggcgat gctggtgcac aacctcagct
      421 tcaagggcgc gggctccagc atgctgtcgc gtccgcgcgc gtacccggac ttccaggtct
      481 cgtcgatggg cgtggcgccg tctccgcagt cgctgtcctt cgatgtcgaa gcggcgggat
      541 ctggcgcgca aggaacgcag ccgctctggg gccgcttcat gcgcgcctgc ggcatgggcg
      601 aattcgttcc ggacgacagt tcctatgtcg attattatcc gctctcgccg cccttcccca
      661 gcagcatcag ccagaagttc aactgggacg gcacggcgca tctgctcacc ggcagccgcg
      721 gcaacgtcac gttcagcgcg gaagcgggca aggagccgct gttcagcttc gctttcaccg
      781 cgctctataa cgcgccgacg gatacggcgc tgctcgacgt gaccgaccgt ctgaacgact
      841 tccttgatcc ggaaacggtg agcgacaacc agacagcctt tgcgctgttc ggcaagcaag
      901 cgccgctctc cgctctgacc ttcgacctca acaatacgtc gagcttccgc gacaagccga
      961 accttgccag cgtcgatatc accgatcgtc cgcagccgaa cggcaaggcg acggtggaag
     1021 caagccaggt ggccgatgtc gattggtttt cgctttacaa ggcgaagacg ctgggcgcgc
     1081 tgcaattcgc gcatggccga accaagggca acaagatcgc catcggttcg ccatacgtgc
     1141 agctggggga gcccaactac accaatgtca acggcgtgct gcatgtcgag ctgccgctct
     1201 acttcatccg cgcgtcgaat gaccgcgagc ttcgcatccg ggtgtactga tggccgagca
     1261 aactggaacg aagttcatct tcaagagcac gcccggcgtt atcgcgtggc cgtgcacggt
     1321 gcagaagccg ctcaacggcg gcggcttcag cgaggagctg ctcaccgcaa agttcgaagt
     1381 gatcagcgac ggcgactacg ccgagctcgt cggcagcccg ctctcacgcc tgagtacacc
     1441 agacaagccg caaggcgatc tgcctctgct gcagcgcgcg ctgaaaggct tcgaaggtct
     1501 cagcgacgaa gatcacaagg cggtgccgga tgatgtcgcg gtgccggtgc tgctagcaca
     1561 gccttacgtc gtggtcgggc tggtgcgcgg ctacatggaa atgctcggga agcgcgcgac
     1621 aaaaaactga aagccgccgc ccgtgtgctg atgggcgagt attcggacgg cggcgatgac
     1681 accttctttg acgatcttcg gcagtggggg agccctgagt cgaagatcgc caagctcaag
     1741 gcccagcgga aacagaatgc gccggctgaa ttcttcgtcg agcctgaaaa ccgcgacgtg
     1801 gtgcgcgtgt tcatgcgtgc gctcacgcaa tggcgcgggc agtccgtcgt cgccggcaag
     1861 cacctgctgc agctccgatc ggggctcgtc tacgaggcgc tgccgacgat cgccacggcg
     1921 ctccgcgtcg aaatggacga gccgctgctc gatgccctgc gtgtcatgga ggccgaagcg
     1981 ctgctcgtgc atgcccagcg ccaggagcag cttctcaaag gccgctgaac gccagtggag
     2041 gggcgcgtga gtagctccgg cctaacagca cgcttggtgc tactggcgga cgccagcggc
     2101 gtggtgggcg ccggtgatca atcaaccgtc gcaatggaca aggttgccgt ttccgcgaag
     2161 gcagcgggcg atgcggggac gcagatggcg gccggtatcg atgcggcgaa gctcgcaacg
     2221 gacggtctcg cccgctccgc ttcgttggca gctacggtca cgcaggcggg cacagaagcc
     2281 caggtgcagc agattgcgaa gctcacccaa gtgtcagccg cgaatatcag cgccaccgaa
     2341 agcgtctcgg cgctggctgc agcgcaatcg gcctacaaca ccaccgtcgc acaagcacgc
     2401 gttctgctcg catcgggtct cctttcggag cagggctacg cgcaagctgt cgagggcgct
     2461 accgccaaac tggccctttc gcgggcgatg cacgatctaa gctccacgag tttgcgggcg
     2521 gaagccgcgg cggccgcggc cgttggcgcg gcgctcgcgg gcttcggaga ggcagaggtt
     2581 gccgcgggca caaacacggc agcattcact gagctcatcc gcgcggcgag ccgtgaggcc
     2641 ggcgtcggcc gcgagaaatt cgccgcgctg catgccagct gggaactgtt ggcggataac
     2701 cccgtggcgg cgatcattgc cgcggcagct gtcgcgacgg tcgccggcgt agtggcctgg
     2761 gatctgtacg atacagccga gcggcgcgtc gaaggcacag cggtgtcgct cggcaacatc
     2821 ttcaatctaa gcaggcaaca gctcgccgcc ttcgccgacc agatcgcgga ggccgcggat
     2881 gtgtcggagc gctcggggac gcaaatggag agctcgttcc tctccgcgaa cctgtcgccg
     2941 gatttatgga atgccgccgt caccgcatcc atgcacctcg ccaatgtgtg gcgaaccgac
     3001 gtgcccgcgg ccgcacagaa actggcggaa gcgctgaaag accccaaggg cgcgggcgag
     3061 cagctgctga gttcgctgaa cgcgctcgac ccgcaaataa agcagctgat cgacgatctc
     3121 acggaacgcg gcaactcgga tcaggctgca gacaaaattc tggagcgcgt caaccagcaa
     3181 ctgcgcgata tgcccgatac gtcttcgaac atctcgcgtg tgttccacca gatggaagcc
     3241 gatctcggca cgatggtgtc gaatgccgga aagctgttcg acacgctcac gaacttccag
     3301 ccgccggcgt ggctgctgtt tcttgagaga caggccaacg ttgccgccaa tctcgcatcg
     3361 gggaatctgc tgggcgccgg cgccgcggcc accgtgccga atgcctccac cccagacgcg
     3421 actgtctccg ttaccggcga gcccggcggt gcgattagta gcataaccag cgaccttgca
     3481 cgccgacaga accaactcgc ccaagactct aacaccatcg atcagtatca gcagagaatc
     3541 gactcggtca aaaacagcat ccacaacttg aatgtcgtgc tgatcgatga ag
//
LOCUS       DATIMH010001110         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3440550, whole genome shotgun sequence.
ACCESSION   DATIMH010001110 DATIMH010000000
VERSION     DATIMH010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3440550"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /gene="rqpR"
                     /locus_tag="VLI90_19180"
     CDS             <1..456
                     /gene="rqpR"
                     /locus_tag="VLI90_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477530.1"
                     /note="The RqpSR system (Regulating Quorum sensing and
                     Pathogenicity Sensor kinase and Response regulator)
                     co-occurs with and modulates the expression of
                     cis-2-dodecenoic acid quorum-sensing systems; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RqpR"
                     /protein_id="HSV16395.1"
                     /translation="KRLKRKAPRVAVLMFSMYREDQYAVRALKAGASGYLSKTVDAAQ
                     MIGAIQQVAAGRKYVSPAMAEALADYVSFDGEQSPHEKLSDREYQTLCMLASGKRLTD
                     IAEALSLSVKTVSVYRTRLLEKMKLRNNAELTFYVMSNRLVDLNPAMAG"
     gene            692..2083
                     /locus_tag="VLI90_19185"
     CDS             692..2083
                     /locus_tag="VLI90_19185"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSV16396.1"
                     /translation="MSLFRKKSVEHMLATSAQNAGLKKALGALDLTFLGVGAIIGTGI
                     FVLTGTGAVQAGPALMISFLIAAVACGFAALAYAEFASTIPVAGSIYTYSYATLGELA
                     AWIIGWDLMLEYGLATSAVSVGWSGYLQSLLSGFGVSLPVALTAAPGALPGHETLFNL
                     PAFLVMMAITALLSVGVRESARINNVMVAIKVTVVLLVIAVGVFHVTPANWHPFMPNG
                     WNGVFGAAAVMFFAFIGFDSVSSAAEEVKNPKRDLPIGIIASLGVCAVLYVAVAAVVT
                     GIVPSAQFANVSHPVSYALQVAGQPWVAGFIDLGAVLGMLTVILVMAYGQTRVIFAMS
                     RDGLLPARLSRVHPRFATPFFTTWLVGIFFGLIGALVPLNVLAELINIGTLAAFSMVS
                     IAVLVLRRTHPELPRAFRCPGVPVVPVLAVASCLFLMVNLQAITWVAFLVWLVVGMGI
                     YFGYSRRRSHLAR"
BASE COUNT          295 a          613 c          743 g          486 t
ORIGIN      
        1 aagcgcctga agcgcaaggc gccgcgcgtg gccgtgctga tgttctcgat gtaccgcgag
       61 gaccagtacg cggtgcgcgc gctgaaggcc ggcgcgtccg gctacctgtc gaaaacagtc
      121 gacgccgcgc agatgattgg cgcgatccag caggtggcgg cgggccgcaa atacgtgagc
      181 ccggcgatgg ccgaagcgct ggccgactac gtatcgttcg acggcgagca gtcgccgcat
      241 gaaaagctct ctgatcgcga atatcaaacg ctgtgcatgc tcgcgtcggg caaacggctg
      301 accgacattg ccgaggcgct gtcgctgtcg gtgaaaacgg tgagcgtgta ccgcacgcgc
      361 ctgctcgaga agatgaagct gcgcaacaac gccgaactca ctttctatgt gatgagcaac
      421 cggctcgtcg acctcaatcc ggcgatggcg gggtaagctg gcggctgcgg caggtcggat
      481 gccgccgggt tgccgccggg ttgccgccgg gttgccgctg gattgccgct ggattgccgc
      541 tgcgatgccg ctcggtcacg ccagcgccac gtatccagag tcccgtcttt ttagtgcgtc
      601 ccaaaaaggt gcatgcgcgc gcatccgcta aaatcgcggg tttcccgaca ttcggcgcgc
      661 gacacgcgtg cactactgga gacccccgcc aatgtccctg ttccgcaaga agagcgtcga
      721 gcacatgctc gcgacgagcg cccagaacgc cggcctgaag aaagcgctcg gtgcgctcga
      781 cctgacgttt ctcggcgtcg gcgccattat cggcaccggc attttcgtgc tgaccggcac
      841 gggcgccgtg caagccggcc cggcgctgat gatctcgttc ctgattgcgg ccgtcgcgtg
      901 cggtttcgcg gcgctcgcct acgccgaatt tgcatcgacg attccggtcg cgggctccat
      961 ctacacgtat tcgtacgcga cgctcggcga actggccgcg tggatcatcg gctgggatct
     1021 gatgctcgaa tatgggctcg cgacgtccgc ggtttcggtg ggctggtccg gttatctgca
     1081 atcgctgctg tccggtttcg gcgtgtcgtt gccggtcgcg ctgacggcgg cgccgggcgc
     1141 gttgcccggc catgaaacgc tcttcaatct gcccgcgttc ctcgtgatga tggcgatcac
     1201 cgcgctgttg tcggtcggcg tgcgcgagtc ggcgcggatc aacaacgtga tggtcgcgat
     1261 caaggtgacc gtggtgctgc tcgtgatcgc ggtgggcgtg tttcatgtga cgccggcgaa
     1321 ctggcacccg ttcatgccga acggctggaa cggcgtgttc ggcgcggccg cggtgatgtt
     1381 cttcgcgttc atcggcttcg actcggtgtc gtccgcggcc gaagaggtga aaaacccgaa
     1441 gcgcgacttg ccgatcggca tcatcgcgtc gctcggcgtg tgcgcggtat tgtatgtcgc
     1501 ggtggccgct gtggtgaccg gcatcgtgcc gtcggcgcag ttcgcgaacg tgtcgcatcc
     1561 ggtgtcgtat gcgttgcagg ttgcgggtca gccgtgggtg gcgggcttca tcgatctggg
     1621 cgcggtgctc ggcatgctga ccgtgatcct cgtgatggct tacggacaga cgcgcgtgat
     1681 cttcgcgatg tcgcgcgatg gtttgttgcc tgcgcggctt tcgcgtgtgc atccgcggtt
     1741 tgcgacgccg ttcttcacga cgtggctcgt cggcattttc ttcgggctca tcggggcgct
     1801 cgtgccgttg aatgtgctgg cggagctgat caacatcggc acgttggcgg cgttttcgat
     1861 ggtgtcgatc gcggtgctgg ttttgcgtag gacgcatccg gagttgccgc gcgcgttccg
     1921 gtgtcctggt gtgccggtgg tgccggtgct tgctgtcgct tcgtgtctgt ttttgatggt
     1981 gaatttgcag gctattacct gggtggcttt tttggtttgg ttggtggtgg ggatggggat
     2041 ttattttggg tattcgagga ggcggtcgca tttggcccga tgagggcagg ggtttttgtt
     2101 gttgttgttg ttagtaaccc ccttggcctt tccttgt
//
LOCUS       DATIMH010001111         4208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4308781, whole genome shotgun sequence.
ACCESSION   DATIMH010001111 DATIMH010000000
VERSION     DATIMH010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4308781"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VLI90_19190"
     CDS             complement(<1..943)
                     /locus_tag="VLI90_19190"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16397.1"
                     /translation="MLQIESLEGRRLLSVAAPIVHAALRGHALFVVGTPGDDVIDVGL
                     VPAAGGDVAHIAVYGTNGVTPSAFSFPAARVRRVIVMAGAGNDTVDTTNASTSFAVNL
                     PAGAVALNVPVVIAGGAGNDVLFGSSQNDTINGGPGNDAISGGAGDDLLLGGPGADGI
                     NGGAGDDRIVGGPGADDFLTSDFPTELIHPDRLDSIFQQATQGLLPVSGRAAVLLGTS
                     TPATLDLTNNELITTDQQYLVAIDSGKVYTAAPGGASDSGGGATTYYSASDGSGIHYT
                     LKGDQNLDGQVDVGDLGALAASYGITGGMSWANSGFNS"
     gene            complement(1277..2929)
                     /locus_tag="VLI90_19195"
     CDS             complement(1277..2929)
                     /locus_tag="VLI90_19195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV16398.1"
                     /translation="MAKYRIDSIANLARQLSFTPVDPRAAQLNAAELLLHELAPDRAY
                     PLDFIIYRITGYRPKLSSLDGDLLTGFALQHDLGLLIEQVSETLDVQTQTIAEPVLAI
                     DDVTEKFNCTSKTIQRWRRRGLPARRFVFPDGKRRVGFLLSSVDRFLAAHQDQLTTGR
                     ANFSQVDDTERDRILRRARRLATQGDCCEQEITRRLGRAMQRSPLTILHTIRKHDAEH
                     PDAAVFPIAPPPLTDEQRAKILKGHKRGLTLGRLARRVHRSRATVYRAIFDERVDRLG
                     KRKIRFIDDPLYHDADAGHLIDDIVQQQELPLEPKPEDARVPRDLPPYLRELYREPLL
                     TPARERALFLKFNFHKYQFVSARRKLEPQFARRRDLDILESHLRHATATKNQILRANL
                     RLVVSVARKHLRPGLNLMELISEGNLTLMRAVEGFDIHKGNRFSTYATLALMKGFARS
                     VPLMLASHKGSTDAAILSEVADPRHQLAAERHIDRDQVRQLLTHLDERERRVLLAHYG
                     LENAESVPATYEQVGAALGLSKQRVRQIEQTALAKLRTAAIQ"
     gene            complement(3149..>4208)
                     /locus_tag="VLI90_19200"
     CDS             complement(3149..>4208)
                     /locus_tag="VLI90_19200"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV16399.1"
                     /translation="TFTAIVQDFLGNSSGGGYDLELDVAPASQATDSNGDGGTIANTQ
                     TKIGTINRFGDLDVFTFTASNNDSFDVTLGDATATSTVHPYLQVFGPTGTRIINTGTS
                     ATTPSVGQTYHIPSTGGSGTYTIIVQDFLGTSSGGTYDLSLTGNIHPLGPQISIVKPA
                     NQTAIALQSKSITLGSFSETNATGPYKVDVNWGDGSADSIFTVTNPGTIPATSHTFPK
                     AGTDTVTEFITDAKSNKSAKVSFTVTVSAATATIGGEVFNDVNANGKIDTGELGLGLW
                     TVYLDLDKSGTLTASDKKVTTDINGKWSFTGLGAGTYTVRIVQVAGSTTTKPTGAVMT
                     ITVVAGQTSSGNLFGEKS"
BASE COUNT          669 a         1260 c         1479 g          800 t
ORIGIN      
        1 tggagttgaa gccgctgtta gcccagctca tcccgccggt gatgccgtag ctggccgcaa
       61 gcgcgccaag gtcaccgacg tcgacttgcc catccaggtt ttgatcgccc ttcagcgtgt
      121 agtgaattcc gctcccatcg ctggccgagt aataggtcgt ggcgccgcca ccgctgtcgc
      181 tggcgccgcc gggagcagcg gtgtagacct tcccgctatc gatggcgacg aggtattgct
      241 gatcggtggt gatcaactcg ttgttggtca ggtccagcgt cgccggcgtt gacgtgccga
      301 gcagcacggc cgcccggccg ctgacaggaa gcaggccttg ggtcgcttgc tgaaaaatgc
      361 tatcgagccg gtcggggtgg atcagctcag tggggaaatc gctggtcaga aaatcgtcgg
      421 ccccgggacc cccgacgatc cggtcgtcgc cagcgccgcc gttgatccca tcggcgccgg
      481 ggccaccgag cagcaggtcg tcgccggcgc caccggagat cgcgtcgttc ccggggccgc
      541 cgttgatcgt gtcgttctga ctcgagccga acagcacatc gttgcccgcg ccgccggcga
      601 tgacgaccgg cacgttgagc gcgaccgcgc cggcgggcag atttacggcg aagctggtgc
      661 tggcgttggt ggtgtccacc gtatcgttgc ccgcgccggc catcacaatg acccgccgca
      721 cgcgcgcggc gggaaacgag aacgcggacg gcgtcacgcc gttggttcca tagacggcga
      781 tgtgcgcgac gtcgccgccg gcggcgggga cgaggccgac atcgatcacg tcatcgccgg
      841 gcgtgccgac gacgaacagc gcatggccgc gcaacgcggc gtgaacgatc ggcgccgcga
      901 ctgacaacag ccggcggcct tcgagcgatt cgatctgcag cataatgagc ccctgattcg
      961 ccatggttga aatgagcgga tggcgaagag gacgcagttt aatgcgtgtc cgcgctgctt
     1021 ggcaaatgtg cgcgcgggac gggacaacgc gggtgtcact tcgagcggga tggacggcct
     1081 gcaaagccgc cgccagtggc ggcggggagg ccgcgatcca ttggtcgcgg ctttacatgg
     1141 cgggggcaac cggcggattg ggtgcaatat taagatgctg tcatcttcag aaagccgagg
     1201 gcctggtcgt gggatgggat ttggagaatt cgtcccacgg cggagccgtg ggctttggtg
     1261 tggaatccgc gcgcctttat tgaatggcgg cggtgcgcag tttggctagc gcggtctgtt
     1321 cgatctggcg gacgcgctgc ttcgataatc ccaacgcagc gccgacttgc tcgtacgtcg
     1381 ccgggacgct ctcggcgttt tccaggccat agtgcgcgag cagcacccgg cgttctcgtt
     1441 catccaaatg cgtcagcagc tgtcgcacct gatctcggtc gatgtgccgt tcggcggcca
     1501 gctggtgccg cgggtcggcg acttcggaca agatcgccgc gtcggtcgag cccttgtgcg
     1561 atgccagcat cagcggcacg ctgcgggcga agcccttcat cagcgcgagc gttgcgtatg
     1621 tgctgaagcg gttgcccttg tggatatcaa aaccttccac ggcccgcatc agcgtgaggt
     1681 tgccttcgct gatcagctcc atcaggttca gccccgggcg aaggtgcttg cgggcgacgc
     1741 tgacgaccag gcgaaggttc gcgcggagga tctggttctt cgtcgccgtc gcgtggcgca
     1801 ggtgcgattc gaggatgtcc agatcgcgcc ggcgggcgaa ctgcggctcg agcttccgtc
     1861 gggccgagac gaactgatac ttgtggaagt tgaacttcag gaacagcgcc cgctcgcgcg
     1921 cgggcgtcag cagcggttcg cgatacagct cgcgcagata cggcgggaga tcgcgcggca
     1981 cgcgcgcgtc ttccggcttc ggttccagcg gcagttcctg ctgctggaca atgtcatcaa
     2041 tcaaatgccc ggcgtcggcg tcgtgataga gcgggtcgtc gatgaagcgg atcttgcgct
     2101 tgcccaggcg atcgacgcgc tcgtcgaaga tcgcgcgata gacggtggcg cgcgaccggt
     2161 ggacccggcg ggcgagccgg ccgagggtca agccgcgctt gtgtcctttg aggatcttcg
     2221 cgcgctgctc gtccgtcagc ggcgggggcg cgatcgggaa taccgcggcg tccgggtgtt
     2281 cggcatcgtg cttgcggatc gtgtgcagga tcgtcagcgg cgagcgctgc atcgcacggc
     2341 caagccggcg ggtgatctcc tgctcgcagc agtcgccttg cgtcgcgagg cgccgcgccc
     2401 ggcggaggat cctgtcgcgc tcggtgtcgt ccacctgtga gaaattcgcg cggccggtcg
     2461 tcagctggtc ctgatgcgcg gcgaggaagc gatcgacgct ggaaagcaga aagcccacgc
     2521 ggcgtttgcc gtctgggaag acgaaccgcc gggccggcag cccgcggcgt cgccagcgct
     2581 ggatggtctt gctggtgcag ttgaattttt cggtgacgtc gtcgatcgcg agcacaggct
     2641 cggcgatcgt ctgtgtctgc acgtcgagcg tttcgctgac ttgttcgatc agcagtccga
     2701 ggtcgtgctg cagcgcgaag ccggtgagca ggtcaccgtc gaggctgctg agcttggggc
     2761 gatagccggt gatgcggtag atgatgaagt cgagcggata cgcgcggtcg ggggcgagct
     2821 catgcagcag cagttcggcc gcgttcagct gggccgcgcg cggatcgacg ggggtgaagc
     2881 tgagctgccg ggcgaggttg gcaatgctgt cgatccggta cttcgccaca acgctgaatt
     2941 atccgcctga ggtagagggg ggtcaaggga aataaaaagg tgcgtaacag tgcaattaaa
     3001 ggcccgcagc aggcgcagcc gctgcacgat ttaccccccc tcgccccctc gccccctcac
     3061 cccctcaacg cgaggcaccc accggaacga gggacatccc tcgctccagc gtgcctctga
     3121 tgatcacaac gcctacacca acgcgtcttc acgacttctc gccgaacaga ttcccgctgg
     3181 aagtttggcc cgcgacgacc gtgatcgtca tcaccgcgcc ggtgggtttc gtcgtcgtcg
     3241 atcccgccac ctgcacgatc cgcaccgtgt atgtcccggc gcccaggccg gtgaaggacc
     3301 actttccgtt gatgtcggtg gtgaccttct tgtccgacgc ggtgagcgtg ccgctcttgt
     3361 ccaaatcgag atacacggtc cacaggccca ggcccaactc gcccgtgtcg atcttgccgt
     3421 tggcgttcac gtcgttgaac acctccccgc cgatcgtcgc ggtggcggcg gagaccgtga
     3481 cggtgaagct taccttcgcc gacttattgc tcttggcatc ggtgatgaac tccgtgacgg
     3541 tgtcggtgcc ggctttgggg aacgtgtgcg acgtcgcagg gatcgtgccg ggattggtga
     3601 cggtgaagat cgaatcggcc gacccgtcgc cccagttcac gtcaaccttg tacgggccgg
     3661 tcgcgttggt ctcgctgaag ctgccgagcg tgatcgattt ggactgaagc gcgatcgccg
     3721 tctgattcgc tggtttgacg attgagatct gcgggcccag cgggtggatg ttgccggtga
     3781 gggacaggtc gtacgtgccg ccgctggagg tgccgaggaa gtcttggacg atgatcgtgt
     3841 acgtgccgct gccgccggtg gaggggatgt gataggtctg tccgaccgac ggggtggtgg
     3901 cggatgtgcc ggtgttgatg atgcgcgtgc cggtggggcc aaatacctgc aggtacggat
     3961 gcaccgtgct ggtggcggtg gcgtcgccga gcgtcacgtc gaacgaatca ttgttgctgg
     4021 cggtgaaggt gaagacgtcc aggtcaccga agcggttgat cgtgccgatt ttggtctggg
     4081 tgttggcgat ggtgccgccg tcaccgttgc tgtcggtggc ctgactggcg ggggcgacgt
     4141 ccagttccaa gtcatacccg ccgccgctgg aattgccgag gaagtcctgc acgatggcgg
     4201 tgaacgtt
//
LOCUS       DATIMH010001112         1659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_555357, whole genome shotgun sequence.
ACCESSION   DATIMH010001112 DATIMH010000000
VERSION     DATIMH010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555357"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VLI90_19205"
     CDS             complement(<1..787)
                     /locus_tag="VLI90_19205"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSV16400.1"
                     /translation="MTIKKLLVTAALAASPVLLIAQGRGLDPAKLLKPLADEWPTYSG
                     DYTAKRYSLLTQINRLNVKHLTLAWTLRLNAGSANGPGGGGFGGGRGGGGATNFNVGG
                     EGNGDYPAGGPPTIKASALMVDGTIYMTAPDNAWAIDARDGRVLWQYFWKSRGGTHIA
                     TRGFGMWKDYLYMETPDNYLVSLDAKTGKERWHKVIADFNEQYFSTAAPVVIGNHVIA
                     GTGNDLDMPGFLQSFDPETGELQWKLYTVPMNPGDPGVETWPSL"
     gene            complement(784..>1659)
                     /locus_tag="VLI90_19210"
     CDS             complement(784..>1659)
                     /locus_tag="VLI90_19210"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSV16401.1"
                     /translation="SPQQPAPPAPQPPDTTGRGGRGRGQGGGQGGGRGFATFPAQQRQ
                     LADPETIAKGKGLFTTNCSTCHGVDLRGGVTGGPNLLRSSVVLMDQHGELILPIVHGA
                     RAERGMPPLPLPDADVVAIAEYIHSVVFTARGQGAPPENEAPPPNALVGDAKAGEAYF
                     TAHCSSCHSPTGDLAGIGSRIDEAKALQNYWIVGGGGGGRGGRGAAGPSERRTVTATV
                     TMPNGEKVQGQVLRLDNFFITLLLDDGRQRTIRREGDVPKVDVKDPLEFHKKVPAILT
                     DKDMHDVTAYLATLK"
BASE COUNT          265 a          531 c          532 g          331 t
ORIGIN      
        1 cgaggctcgg ccacgtctcg acgccgggat cacctggatt catcggaacc gtatagagct
       61 tccactgcag ttcgcccgtt tccggatcga acgactgcag gaaccctggc atgtcgagat
      121 cgttgcccgt cccggcaatc acgtgattgc caatcacgac gggagccgcg gtcgagaagt
      181 attgctcgtt gaagtcggcg atgaccttgt gccagcgctc ttttccggtt ttcgcgtcga
      241 gcgacaccag atagttgtct ggcgtctcca tgtagaggta gtccttccac atgccgaagc
      301 cgcgcgtggc gatgtgcgtg ccgccgcgcg atttccagaa gtactgccac agcacacggc
      361 cgtcgcgcgc atcgatggcc caggcgttgt caggcgccgt catgtaaatg gtgccgtcga
      421 ccatgagcgc tgatgctttg atcgtcggag gaccgccggc cggataatcg ccattcccct
      481 cgccgcccac gttgaaattt gttgcaccgc cgccgccgcg gccgccgccg aatccaccgc
      541 cgccaggccc gttggcggaa ccggcattga ggcgcagcgt ccacgccagc gtcaggtgtt
      601 tgacgttgag acgattgatt tgcgtcagca ggctgtagcg cttggccgtg tagtcgccgc
      661 tgtaggtcgg ccactcgtcg gcaagcggct tgagaagttt cgctggatcg agcccgcgtc
      721 cttgcgcgat caggaggacg ggcgaagccg ccagcgcggc ggtcacgagc agtttcttta
      781 tggtcatttg agtgtcgcga gataagcggt tacgtcgtgc atgtctttgt cggtcaggat
      841 cgcgggaacc ttcttatgga actccagtgg atccttcacg tccactttcg gcacgtcgcc
      901 ctcgcgccga atcgttcgct gccggccatc gtcgagcagc agcgtgatga agaagttatc
      961 gagccgcaga acctggccct gaactttttc gccattgggc attgtcacgg tcgcggtcac
     1021 ggtgcggcgt tcgctcggac cggctgcgcc gcggccgcct cttccgccgc cgccgccacc
     1081 gacgatccaa taattctgta gcgcctttgc ctcgtcgatg cgggatccga tgccggcgag
     1141 atcgccggtc ggcgaatgac agctgctgca gtgggcggtg aagtacgctt caccggcctt
     1201 ggcgtcgcca accagcgcat tcggcggagg ggcttcattt tccgggggcg ccccctgtcc
     1261 gcgtgcggtg aagaccacgc tatggatgta ctccgcgatc gcgacgacgt cggcatcggg
     1321 tagcggcagc ggcggcatgc cacgttcggc acgcgcaccg tggacaatcg gaagaatcaa
     1381 ctcgccgtgt tgatccatca acaccacgga cgagcgcagc agattgggtc cgcccgtcac
     1441 accgccgcga agatcgacgc catggcaggt gctgcagttg gtcgtgaaga gtcctttgcc
     1501 tttggcgata gtttcagggt ccgcaagttg ccgctgctgt gcgggaaagg tcgcgaaccc
     1561 tcgcccgcca ccttgtccgc cgccctggcc ccgaccgcgt ccgccgcgcc ccgtcgtgtc
     1621 gggcggctgc ggcgccggag gcgccggttg ctgcggtga
//
LOCUS       DATIMH010001113         5649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2864503, whole genome shotgun sequence.
ACCESSION   DATIMH010001113 DATIMH010000000
VERSION     DATIMH010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2864503"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2476)
                     /locus_tag="VLI90_19215"
     CDS             complement(<1..2476)
                     /locus_tag="VLI90_19215"
                     /inference="COORDINATES: protein motif:HMM:NF045351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16402.1"
                     /translation="MKLDWLDRSPPATTQPLSWGVPWPQGQVKKDATFALSGGDGHGL
                     PLQSWPLAYWPDGSLKWTAHATVVPGGENGLSISVSPAAAAATSLHVESVGDDIAIDT
                     GPMVCHLAKNGPSLIKSIALGDKIVARNGRLLCSIDAASGEESFTSQIDNVIVEQTGP
                     VRAVVKFTGKHKSTTGDRSWLPFVVRLYFYAGDDSIRMVHTFIFDGDQDKDFIRSLGV
                     RFAVPMREQIHNRHVRFAGETGMFAEPVQLIAGRRNRRPDLYRQQVAGQRVPNLDQLP
                     DADLVKDMAVWDRYRLTQISADSYQIQKNTNDQSAWINASAANRSLGLAFVGDVSGGL
                     AVGLKNFWQLAPTELEIIGASTDAAQLTLWLWSPQSPRMDLRHYDTKAHGLEASYEDV
                     QPGFSTATGIARTSEITIHPFNNVPSNERLLAVAKSNAQPPMLVCTPEYFHSLPVFGL
                     WSLPDRSSPGKRWIENELDKAIAFYQGQIEQRRWYGFWDFGDVMHTYDTTRHEWRYDI
                     GGFAWANTELMPNLWLWYSFLRSGRADVYRMAEAMTRQTQEVSVYHLGRFAGLGSRHN
                     VRHWGDGAKELRISQALLKRFYYYLSTDERTGELMNEVIDADAKLVDVDPMREIVGKS
                     QYPTHMRVGPDWFAAVSNWYTAWERTGDAKYRDKILAGMKSIVAMPKKIFAGGITNVA
                     GGTFGYDPKTGKLYQLADKADVPPLAALFGGPELNFEIVPVLNDADWAQAWLQYCELL
                     NAPRAEQEKVLGNAINSGRSADFSRMAAYAAYVKHDPQLAARAWQQFLHAGNLGGAAP
                     GTEAPLDPFDVHKINGPQAPTPVDEIR"
     gene            complement(2668..4722)
                     /locus_tag="VLI90_19220"
     CDS             complement(2668..4722)
                     /locus_tag="VLI90_19220"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16403.1"
                     /translation="MHVWGLHVVDFAILLLFLLAILAIGFKVSHGVKEESDFYLGGRK
                     LGRVLQFFLNFGNATDSTGAVQTSSEVFRQGASGVWLGLQTLFITPFFWFTQPWYRRA
                     RLVTMADLFVDRFNSKSLASAYAAFNILIALITLGTGNIAAYKVAAAMIVKEPASYTD
                     TDRQRVEHYNEYQRLKAQRIAGTMPQSAADQFRYLNDLNARGELSSDVPYFGKVPFLI
                     GYSAIVAAYIILGGLKAAAITDALQGVLVLLMSILLIPLGLHRIGGFHEMHLRVPAFK
                     FQMFGDAAVSEYAWYTIAAITFASLVQILGLMHNMSAGGSARDEDTARFGMISGGFMK
                     RFILIAWMFCGLIAIALLGGSLADPDAAWGTLSKSLLMPGLMGIMLSGMLLGHMPAVG
                     VTSVAVSALATRNLYEPLVPGRSERHYLRIGQLAIAVVLGVGIAVATFFTGVAQAWTM
                     MITFNTYFGAVVFLIFFWRRLTPSAIMIGLVAWVILLGVVPWFLPTSQAFRRQPAFLQ
                     KTNERQVIDEEHKSRTILPKAIYFDNVAHANPNDPTSALEGIGRFNIETYSLHLIGVP
                     MASFSPAQLLATRWAFDGIFPFIMLIVLSWLTPRSQPQRADFFYAKLKTPVGKTPEAD
                     EREVRLSFDEPHRFDDQKLLPRTNWEFTKWTRKDVVGFFGCWAIVGVILLVLWAVLRV
                     GA"
     gene            complement(4867..>5649)
                     /locus_tag="VLI90_19225"
     CDS             complement(4867..>5649)
                     /locus_tag="VLI90_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16404.1"
                     /translation="AQTTQPQAVAGASPSSALKLSVDFEENPSALSDGLTARVWHPKP
                     LPSQEKNPGKIGDIAEVGQGENTIDTSVFGDGKHYLYIGADGFIEQWLRINIDHGQVA
                     PASASITMFRTRYVVLRYAFNTKGRELTGPNVKTGRMALTHFGMLPYFGEDWQVWQAE
                     SERGFGFGTIPVLAHHRVAEQYGFAPAPDGAKFDDLNTAPTEAAYHPKTYLAEPGLIL
                     FCRVLGNRARPPEQLGYGKVMVEDVTLTKPANVPVKERGLPK"
BASE COUNT         1020 a         1813 c         1771 g         1045 t
ORIGIN      
        1 tgcggatctc gtcgaccggc gtcggcgctt gcggtccatt gatcttgtga acgtcgaacg
       61 ggtcgagcgg cgcttcggtg cccggcgcgg cgccgccgag gttgcccgcg tgcaggaact
      121 gctgccaggc gcgggcggcc aactgcgggt cgtgcttgac gtacgccgca tacgcggcca
      181 tgcgcgagaa gtccgcgctg cggccgctgt tgatcgcgtt gccgagcacc ttctcctgct
      241 ccgcccgcgg ggcgttgagc agctcacaat actgcagcca cgcctgcgcc cagtccgcat
      301 cgttcagcac cggcacgatt tcgaagttca gctccggccc accaaacagc gccgccagcg
      361 gcgggacatc cgccttgtca gccagctgat agagcttgcc cgtcttcggg tcatagccga
      421 atgtcccccc cgccacgttc gtgattcccc cggcgaagat cttcttcggc atcgcgacga
      481 tcgacttcat gccggcaagg atcttgtcgc ggtacttcgc atcccccgtg cgttcccacg
      541 cggtgtacca gttgctgacg gccgcgaacc aatcggggcc gacgcgcatg tgcgtcgggt
      601 attggctctt gccgacgatc tcgcgcatcg ggtccacgtc gaccagcttg gcgtcggcgt
      661 cgatcacttc gttcatcaac tcgccggtgc gctcgtcggt gctcaggtaa tagtaaaacc
      721 gcttgagcag cgcctggctg atgcgcagtt ccttggcgcc gtcgccccag tgccgaacgt
      781 tgtgccgcga acccaggccg gcgaatcggc cgaggtgata aacgctgact tcctgcgtct
      841 gccgggtcat cgcttcggcc atgcgataga cgtcggcccg cccgctgcgg aggaagctgt
      901 accagagcca caggtttggc atcagctcgg tgttcgccca ggcgaacccg ccgatgtcgt
      961 accgccactc gtggcgggtg gtgtcgtacg tgtgcatcac gtccccgaaa tcccaaaagc
     1021 cataccagcg gcgctgttcg atctggccct gatagaacgc aatagcctta tcgagctcgt
     1081 tctcgatcca tcgcttcccc ggcgaggatc gatccggcaa actccacagg ccgaacaccg
     1141 gcagcgaatg aaaatactcc ggcgtgcaga ccagcatcgg cggttgcgcg tttgacttcg
     1201 cgaccgccag cagccgttcg ttgctcggca cgttgttgaa cggatgaatg gtgatttcgc
     1261 tcgttcgcgc gatgccggtc gcggtgctga aaccgggctg cacatcttcg tagctggcct
     1321 cgagcccatg cgctttggtg tcgtaatgcc gcaggtccat ccgcggggac tggggcgacc
     1381 agagccatag cgtcagttgt gccgcatccg tcgatgcgcc gatgatctcc agttccgtcg
     1441 gcgccaactg ccagaagttc ttcagcccca ccgcaagacc gccggagacg tcaccgacga
     1501 acgcgaggcc cagcgatcga ttggccgccg acgcgttgat ccacgcgctc tggtcgttcg
     1561 tgttcttctg gatctgatac gagtcggccg atatctgcgt gaggcgatat cgatcccaca
     1621 ccgccatgtc tttgacgagg tctgcatccg gcagctgatc caagttcggc acgcgctgcc
     1681 ccgccacctg ttggcggtac aagtccggcc gccgattgcg gcgtcccgcg atcagctgca
     1741 ccggctcggc gaacatgccg gtctcgcccg cgaagcgcac gtggcggttg tggatctgct
     1801 cgcgcatcgg caccgcgaat cgcacgccga gcgagcggat aaaatctttg tcctggtcgc
     1861 cgtcgaagat gaacgtgtgc accatgcgga tcgagtcgtc gccggcgtag aaataaaggc
     1921 ggacgacgaa cggcagccaa ctgcgatcgc ccgtcgtcga tttgtgtttg ccggtgaact
     1981 tcacgaccgc ccgcacgggg ccggtctgtt cgacgatgac gttgtcgatc tggctggtga
     2041 acgattcttc gccgctggcg gcgtcaatcg agcagagcag ccgcccgttg cgcgcgacga
     2101 ttttgtcgcc gagcgcgatc gatttaatca gagatggacc gtttttcgcg agatggcaca
     2161 ccatcgggcc ggtgtcgatg gcgatgtcgt cgccgacgga ctcgacgtgc agcgacgtcg
     2221 cagccgcggc ggccggactg acggagatgg aaagcccgtt ctccccaccc ggcaccacgg
     2281 tggcgtgggc ggtccacttc agcgatccgt ccggccaata tgcgagcggc caagactgca
     2341 gcggcaagcc gtggccatcg ccgccgctga gtgcgaacgt cgcgtccttc ttcacctgtc
     2401 cctgcggcca cggcacgccc cagctgagcg gctgcgtcgt cgccggcggc gagcggtcga
     2461 gccagtcgag cttcacatca gcggctcgcg ttgttgcggg aggtaacgca attgccagta
     2521 acaggatcaa cgacgacaaa tgcatcattg cgctgttcat aaaatcctcg tcttgatagc
     2581 cggggccgga aggcccggtt tgttatggga cgaaaagacc gggccttccg gccccggcta
     2641 ataatgtgca atcgccatcg cctaatttca cgcgcccacg cgcaacaccg cccacaggac
     2701 cagcaggatc acgccgacga tcgcccagca accgaagaag ccgacgacat ctttccgcgt
     2761 ccacttcgtg aactcccagt tcgtccgcgg cagcagcttt tggtcgtcga atcggtgcgg
     2821 ctcgtcaaag ctcagccgga cctcgcgctc atccgcttcg ggcgtcttgc cgaccggcgt
     2881 cttcagcttc gcgtagaaga aatccgcccg ctgcggctgg ctccgcggcg tgagccaact
     2941 gagcacgatg agcatgatga acggaaaaat cccatcgaac gcccagcgcg tcgccagcag
     3001 ctgtgcgggg ctgaagctgg ccatcggcac gccgatcagg tgcaacgagt acgtctcaat
     3061 gttgaaccgg ccaatgcctt ccagcgccga cgtcggatcg ttggggttcg cgtgcgcgac
     3121 gttgtcgaag tagatcgcct tcggcaggat cgtccggctt ttgtgctctt cgtcgatcac
     3181 ctgccgctca ttcgtcttct gcaaaaacgc cggctggcgg cggaaggctt gcgatgtcgg
     3241 caaaaaccac ggcaccacgc cgagcaggat cacccatgcg accaggccga tcatgatagc
     3301 gctcggcgtc agccggcgcc aaaagaagat caggaagacc accgcaccga agtaggtgtt
     3361 gaaggtgatc atcatcgtcc acgcctgggc gacgccggtg aagaacgtgg cgaccgcgat
     3421 ccccaccccg agcacgacgg cgatggccaa ttgaccgatg cgaagatagt gacgctcact
     3481 gcgcccgggc acaagcggtt catacaggtt gcgcgtcgcc agcgccgaga cggccaccga
     3541 cgtcacgccc accgccggca tgtgaccgag caacatgccc gagagcatga tccccatcag
     3601 ccccggcatg agaagtgatt tggaaagcgt gccccacgcg gcgtcggggt cggccaggct
     3661 gccgcccagc agcgcgatcg cgatcagccc gcagaacatc cacgcgatga ggatgaagcg
     3721 cttcatgaac ccgccgctga tcatgccgaa gcgggcggtg tcttcgtcgc gcgccgatcc
     3781 accggcggac atattgtgca tgagcccgag gatctgcacg agactggcga acgtgatcgc
     3841 ggcgatcgtg taccacgcgt attcgcttac cgcggcgtcg ccgaacatct ggaatttgaa
     3901 cgccggcacg cgcaggtgca tctcgtgaaa cccaccgatg cggtgcaggc cgagcgggat
     3961 cagcaggatc gacatcagca gcacgagcac gccctgcagc gcgtcggtga tggcggcggc
     4021 tttgaggccg cccaggatga tgtacgcggc gacgatggcc gagtaaccga tcaggaacgg
     4081 caccttcccg aagtagggaa cgtcggaaga cagctcgccg cgcgcgttga ggtcattgag
     4141 atagcgaaac tggtcggccg cgctctgcgg catcgtgccc gcgatgcgct gcgctttgag
     4201 ccgctggtat tcgttgtagt gctcgacgcg ctgccgatca gtgtcggtat acgacgccgg
     4261 ttccttcacg atcatcgcgg cggcgacttt gtaggcggcg atgttgccgg tgccgagcgt
     4321 gatcagcgcg atgaggatgt tgaacgccgc gtacgccgag gcgagcgatt tgctgttgaa
     4381 gcgatcgacg aacaggtccg ccatcgtgac gagccgcgcc cggcgatacc acggctgggt
     4441 gaaccagaag aacggcgtga tgaacagcgt ttgcaatcca agccagacgc ccgatgcacc
     4501 ctgtcgaaag acttcgctgg acgtttgaac cgcgccggtc gaatcggtcg cgttgccgaa
     4561 gttgaggaag aactgcagga cgcgcccgag ctttcgcccg ccgaggtaga agtcggattc
     4621 ttctttgacg ccgtgcgaga ccttgaaacc gatcgcgagg atcgcgagga ggaacagcag
     4681 gaggatggcg aagtcgacga cgtgtagacc ccaaacgtgc acgtgatcct ccgccgactg
     4741 agctgaccca ccgtcgcggg aagtgtacgg agtgccgcgg cggaagggaa taggcctcga
     4801 tagctgattc tcaccaattc ccgttatttc ccaagctgac ttgacgcaga agtggccgat
     4861 gcgcttctac ttaggcaggc cccgctcctt caccgggacg ttcgccggtt tggtgagcgt
     4921 cacgtcttcc accattacct tgccgtagcc aagctgctcc ggcggtcgcg cgcgattgcc
     4981 gagcacgcgg cagaataaga tcaaacctgg ctcggcgagg tatgtcttgg ggtgatatgc
     5041 agcctcggtc ggcgcagtat tcagatcgtc gaacttcgcc ccatccggtg ctggcgcgaa
     5101 gccatattgc tcggccacgc gatggtgggc cagcaccggg atcgtgccga acccgaagcc
     5161 tcgttcgctc tcggcttgcc agacctgcca gtcttcgccg aagtacggca gcatgccgaa
     5221 gtgcgtcagc gccatccgcc ccgtcttcac gttcggtccc gtcaactcgc ggcccttggt
     5281 gttgaaggca tagcgcagca cgacatagcg cgtgcgaaac atggtgatcg acgccgacgc
     5341 cggcgcgact tggccgtggt cgatgttgat tcgcagccat tgctcgatga atccgtcggc
     5401 tccgatgtag aggtaatgct tgccgtcgcc gaagacgctg gtgtcgatcg tgttctcgcc
     5461 ctgcccgacc tccgcgatgt cgccgatctt gcccgggttc ttctcctggg atggcagtgg
     5521 tttcggatgc cacacgcggg cggtcaagcc gtcgctcagc gcgctcggat tctcctcgaa
     5581 atcaacgctc agtttgagcg cggacgatgg cgatgcgccc gcgaccgcct gtggctgcgt
     5641 cgtctgtgc
//
LOCUS       DATIMH010001114         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_845591, whole genome shotgun sequence.
ACCESSION   DATIMH010001114 DATIMH010000000
VERSION     DATIMH010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845591"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VLI90_19230"
     CDS             complement(<1..400)
                     /locus_tag="VLI90_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16405.1"
                     /translation="MNQRIVKPPACNMGVREELTRRPWIAVVTVAIGFTIIALNLFSR
                     GGTNAQPTAPSRVYFSDDDGKTFFADDAALIPPFDHNGHTAVMAMVFKCPGKQPFIGY
                     LLRFNHEGRTALAALSESQRRSADAKVIGIK"
     gene            complement(438..1295)
                     /locus_tag="VLI90_19235"
     CDS             complement(438..1295)
                     /locus_tag="VLI90_19235"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV16406.1"
                     /translation="MSNVKKRAGFTLVELLVVIGIIALLIGILMPALTKARAAAIRTV
                     CLARMRELGNAAMLYAANNRGSLPPIWNYCDVPPVHFGRPAIYSTTGTPETDCYLTKY
                     LGKGDTAKRYICPALQADAGYSLTSGQSYRYNQILGGYRSKSEMTQTAPGSGYYYANP
                     WRIGQIRQSSRMALFLDTDLVVGGFGNGGNAIWFRQDSSTVSSTPGASYHWPNQQGTL
                     QPHGKFQPLKSYVGYLGLTYPGIGGLTNVAFVDGSVRLVPFRIDASPVKFQGSDCMFV
                     DPNHPTPMW"
     gene            complement(1481..2701)
                     /locus_tag="VLI90_19240"
     CDS             complement(1481..2701)
                     /locus_tag="VLI90_19240"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSV16407.1"
                     /translation="MSRPAPDTVPTKSARRHAAPPRRKFIALRLSAWSDWDRQVIRGV
                     QRFAHDRPDWRMYVEGGDNVSSKTFAAELTLDGLITSVLRDMSPAWRQLIKRLDPCVV
                     AISSAVPQRLSNIPRVRVDDNKVAASIGRHFLAGGFRHLAYFALPQPGAVVEDTRLKA
                     IGDFAAREGCPFHLLPAPKVRGELSAGERMRWIQQLPKPIGIAAWNMQEARRLVTALD
                     RAGIAVPEQVAVVAWDDDPMLAETLEPTISAAVLPAERLGFEAAQLLDRMFSGAPRPV
                     APVVIEPTGVLRVRQSSDVSTLADRDAHLAMQYIREHGTEPIKVTHVASALRLSRRSL
                     EVVFSRVSGKTLHDAIMEVRLERARQLLAETDWPLARVADRAGLGTEQTLRRLFLSKE
                     QMTPGAYRARFSGV"
     gene            2877..3953
                     /locus_tag="VLI90_19245"
     CDS             2877..3953
                     /locus_tag="VLI90_19245"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HSV16408.1"
                     /translation="MRRLNGLYILDSDALELTYGPAERRDIERYVDFVAPAQTRATIA
                     ADRSLLKHVEVIFSGWGAPLLDDVFLDAAPNLKAFFHAAGSVGYCVTDEMWERGVMVT
                     TANAANAVPVAEYTLGTILFSLKHGWKLMRQTREQRTFVERDGAPGAYGSTIGLISLG
                     AMGRAVLARLAPFDFNVIVHDPYIDAAEAKALGVELVSLAELFKRADVVSLHSPSLAE
                     TQGMITGAHLASMKPGATFINTSRGEIVREDELLAVAAERADLQFVLDVVATEPPPVD
                     SPLYTLANIVLTPHIAGSVGNECRRMGRYMVQELERFVAGKPLKWLVTPESSRNTTHR
                     PVTVKTFAKGSKAPRHSAPPLQSV"
BASE COUNT          727 a         1304 c         1299 g          722 t
ORIGIN      
        1 gcttgatgcc gatcaccttc gcgtcggcgc ttctgcgctg cgattcgctc agcgccgcca
       61 gcgctgttcg tccctcgtgg ttgaagcgca gcaggtaacc gatgaacggc tgcttgcccg
      121 ggcatttgaa aaccatcgcc atcaccgcgg tgtgaccgtt gtgatcgaac ggcgggatca
      181 gcgctgcgtc atccgcgaag aaggtcttgc cgtcatcgtc ggaaaagtaa actcggctcg
      241 gcgccgttgg ctgcgcgttc gtgccgccgc gcgagaacag gtttagcgcg ataatggtaa
      301 acccaatcgc aacggtcacc accgcgatcc agggtcttcg agtcagttct tcgcgcacgc
      361 ccatattgca cgctgggggt ttcactatcc gctggttcat ggccgttcat ccaccgcgtc
      421 gcaaatgaga cgcccgttca ccacatcggc gtgggatgat tcggatcgac gaacatgcag
      481 tcgctgccct ggaacttcac cggggacgca tcgatgcgga acggcaccag gcggacggac
      541 ccgtcaacaa acgcgacgtt ggtcaagccg ccgatcccgg gataggtcaa gccgagatat
      601 ccaacgtacg acttaagggg ctggaacttt ccgtgcggct gcagggtgcc ctgctggttg
      661 ggccagtgat agctggcgcc gggcgtgctg ctgacggtgg agctgtcctg gcggaaccag
      721 atggcgttgc cgccgttgcc gaagccgccg acgaccaggt ccgtgtccag gaatagcgcc
      781 atgcgcgatg actggcggat ctgcccgatg cgccacgggt tggcgtagta gtagccggag
      841 ccgggggctg tctgcgtcat ctccgatttc gatcgatatc cgccgaggat ctggttgtat
      901 cggtacgact gcccgctggt cagcgaatag ccggcatccg cttgcagcgc ggggcagatg
      961 tagcgcttgg cggtgtcgcc cttgccgagg tatttggtca ggtaacagtc ggtctcaggc
     1021 gtgccggtcg tgctgtagat cgcgggacgg ccgaagtgca ccggcgggac atcgcaatag
     1081 ttccagatcg gcgggagcga cccacggttg ttggcggcgt acagcatggc ggcgttgccc
     1141 aactcgcgca tgcgggccag gcaaaccgtc cggatcgccg ccgcgcgggc cttggtcaag
     1201 gccggcatga ggatgccgat cagcagggca atgatgccga tgacgacgag caattcgacc
     1261 agcgtgaatc ccgctcgttt tttcacgttg ctcatatccg ttcctcgcaa gggccgttgg
     1321 gaagtgcaat ccgtttccgt ccattcaacc taccgcaccc cgggaggaag tgttacctac
     1381 agtttcagca aaaggaggga aaaacacgta cactttgcgc agcaaatgaa tctgctgcgt
     1441 agtggtcccg tagcacgggc tacgggaccg ctacgcaaat tcaaacgccg ctgaagcgcg
     1501 cgcgatacgc ccccggcgtc atctgctctt tcgaaaggaa caaccgccgg agcgtttgct
     1561 ccgtgcccaa ccccgcgcgg tccgccaccc gcgccagcgg ccagtccgtc tccgccagaa
     1621 gctggcgcgc ccgctccagc cgcacttcca tgatcgcatc gtgcagcgtt tttccagaca
     1681 ctctgctgaa gacaacctcc aggctgcggc gcgacagccg cagcgcgctg gccacgtgcg
     1741 ttaccttgat cggctcggtg ccgtgctcgc ggatgtactg catcgccaga tgcgcatcgc
     1801 gatcggccag tgtcgatacg tcagatgact gccgcacgcg cagcaccccg gtgggttcaa
     1861 taaccaccgg cgccaccggc cgcggcgcgc cgctaaacat gcggtcgagc aactgcgcgg
     1921 cttcgaaccc caatcgctcg gccggcagca ccgcggcgga gatcgtcggt tccagcgtct
     1981 cggccagcat cgggtcgtca tcccacgcca ccaccgccac ctgctcgggc acagcaatgc
     2041 cggcccggtc cagcgccgtg accagccggc gggcctcctg catgttccac gcggcgatgc
     2101 cgatgggctt gggcaattgc tggatccacc gcatgcgctc ccccgccgac agttcccccc
     2161 gcaccttcgg ggccggcagc agatgaaacg ggcaaccttc gcgggcggcg aagtcgccga
     2221 tcgctttcaa ccgcgtgtct tccaccaccg ccccgggctg cggcaacgcg aaatacgcca
     2281 ggtgccgaaa cccaccggcc aggaaatgcc gcccgatgct ggcggccacc ttgttgtcat
     2341 ccacccgcac gcgcgggatg ttcgacagcc gctgcggcac cgccgagctg atcgccacca
     2401 cgcacggatc caaccgcttg atcagctgac gccacgccgg tgacatgtcg cgcagcacgc
     2461 tggtgatcag cccgtccagc gtgagttcgg ccgcgaaggt cttggacgag acgttgtccc
     2521 cgccttcgac atacatgcgc cagtcgggtc gatcgtgcgc gaagcgctgc acgccgcgga
     2581 tgacctggcg gtcccagtcg gaccacgcgg acaatcgaag cgcgatgaac tttcgccgcg
     2641 gcggcgccgc atgacgccgt gccgatttcg tcgggacagt atctggtgcc ggacgactca
     2701 tggatctcct tatgccgcgt gcagcggtcg cgcccgcatg gtgcgataac ctggcccgca
     2761 aactgtagtc gaaatttatc caattcgcgc aaagtgttgg tcatcaatcg ccggtaaatg
     2821 gcgtaccttc tccatgtcga cgtgaccgag ttcagttgcc gagggtggat ttgcacatgc
     2881 gtagactcaa cgggctttac atcctggatt ccgatgcgct ggagctgaca tatggaccgg
     2941 ccgaacgccg ggacatcgag cggtacgtgg atttcgtggc gccggcgcag acgcgagcga
     3001 cgatcgcggc ggatcggtcg ctgctgaagc acgtggaggt gatcttcagc gggtggggcg
     3061 cgccgctgct ggacgatgta ttcttggatg ccgcgcccaa tctgaaagcc ttcttccacg
     3121 ccgccgggtc ggtcggttac tgcgtgaccg acgagatgtg ggagcgcggc gtgatggtga
     3181 caaccgccaa cgccgccaac gccgtgccgg tggccgagta cacgttgggg acgatccttt
     3241 tttcgctgaa gcacgggtgg aagctgatgc ggcagacgcg cgaacagcgc acgttcgtcg
     3301 aacgcgacgg cgcccccggc gcgtacggca gcaccatcgg attgatctcg ctgggcgcga
     3361 tgggccgagc cgtgctggcg aggctggcgc cgttcgactt caacgtcatc gtgcatgatc
     3421 cgtatatcga cgcggccgaa gccaaggcgc tgggcgtcga actggtgtcg ctggcggaac
     3481 tgttcaagcg cgccgacgtc gtgtcgctac attcaccgag cttggccgag acgcagggca
     3541 tgatcaccgg cgcgcacctg gcatcgatga agccgggcgc gacgttcatc aacacctccc
     3601 gcggcgagat cgttcgcgag gacgaactgc tggccgtggc ggccgagcga gcagacctgc
     3661 agtttgtgct cgacgtggtg gcgaccgaac caccgccggt ggattcgccg ctgtacacgc
     3721 tggcgaacat cgtgctgacg ccgcacatcg cggggtcggt cggcaacgag tgccggcgaa
     3781 tgggccggta tatggtgcag gagctcgagc gattcgtcgc gggcaaaccg ctcaaatggc
     3841 tcgtcacgcc ggagtccagc cgaaatacca cacatcggcc ggtgacggtg aagacgttcg
     3901 ccaagggcag caaggctccg cgccattccg ccccgccgct gcagagcgtt tgatcaacga
     3961 ggaactccca aaacactcgt acgccttgta gctgcgacgc ctgcgtcgcc tttgtttttc
     4021 tgagacgaag aggcgacgca ggcgtcgcag ct
//
LOCUS       DATIMH010001115         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2864636, whole genome shotgun sequence.
ACCESSION   DATIMH010001115 DATIMH010000000
VERSION     DATIMH010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2864636"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..>2313)
                     /locus_tag="VLI90_19250"
     CDS             complement(332..>2313)
                     /locus_tag="VLI90_19250"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HSV16409.1"
                     /translation="ACQPAHHDEPASVAPAASTPSSTPALATTPVNFQFHPDAPFATV
                     ASQSGDTAVTFTQHAGRLMFAVTLKKAPAIESSPMVFTVDDVDLTADAEPTRIDSYQI
                     HETYPWVGIHSLAINNCRGQRITLRHKSGARYTLDVRAFDDAIAFRFIVPDAGHPRVP
                     DEATTFTLPAGSIGWFHGLSGHYEGEYARHEIEQVSAGEWAAPPVTFRLPSKHGYVAI
                     TEADLKNYAGMALQADGQRGFTIALGHRQPVSGPFRLRYRASEARRLSVAASVDGEIV
                     TPWRVMVLGSDLDTLVNSDAITSLCPPPDKAIFPQGPATDWVKSGRAVWRYLDGGDQS
                     AQGIRHFFDGAHQLGFEYQIVEGMWRRWSPAELKGIIDYGKQRNVGMFAWVYSKDLHD
                     PAARAALFARCESAGIAGLKIDFFDSEAKELVDLYQAILREGAQHHLLIDFHGANKPT
                     GQRRTWPNELSREAVRGMESSRMTARAFHDTTLPFTRWLAGPADYTPVLFTARRADTT
                     WAHQVATAMTLTSPLLTYGANPDAILQNPAVEMIKSIPCTWDETIVLPASEIGECAAI
                     ARRHGDMWFLAITNGPAARSMKISLSFLDEADCHALIVSDPPNGNGGQVDVKQVDMHS
                     RQPLALDLRPGGGYLARYAKFAPGEKFATTAPIAE"
BASE COUNT          398 a          711 c          773 g          431 t
ORIGIN      
        1 agttggccgc tgaggatcgc ttcctgaatg tggtcgtacg ccttctggac caaagtgcca
       61 ctgacggggg acgaagatgg tgccttctgc gaacgagatg gcatcgctgc tgactccctt
      121 ttttgcaaac ggtgacttgg cgaacggagg ggccatcata ttcggcgcag cgatttccgg
      181 aagcgaagaa atcgcaggat tcggtgaaac gtttaacccg aagaaccaaa acagatgttg
      241 aacgcagaga cgcggaggcc gcagagatcg gattcagatt ctagtcctac ctctgcggcc
      301 tctgcgtctc tgcgttcgca ttctgcattg atcattctgc aatcggcgcc gtcgtcgcaa
      361 acttttcgcc cggcgcgaac ttcgcatagc gcgcgaggta accgccgccg gggcggaggt
      421 cgagggccag gggttgccgc gagtgcatgt ccacttgctt cacgtccact tggccgccgt
      481 tgccattcgg tggatcgctg acgatcagcg cgtggcagtc ggcttcgtcg aggaagctga
      541 gcgagatctt catcgagcgc gcggcggggc cgttggtgat ggcgaggaac cacatgtcgc
      601 catggcgacg cgcgatggcg gcgcattcgc cgatctccga cgccggcagc acgatcgttt
      661 cgtcccacgt gcacgggatg ctcttgatca tctcgaccgc ggggttctgc aggatggcgt
      721 cggggttcgc gccgtaggtc agcagcggcg aggtgagcgt catggccgtg gccacctggt
      781 gcgcccacgt cgtgtcggct cggcgcgcgg tgaagaggac cggcgtgtaa tccgcgggcc
      841 cggcgagcca gcgcgtgaac ggcagcgtcg tgtcgtggaa cgcccgggcg gtcatccggc
      901 tcgactccat cccgcggacg gcctcgcgcg acagctcgtt cggccaggtg cggcgctggc
      961 cggtcggttt gttggcgccg tgaaaatcga tcagcaggtg atgctgcgcg ccctcgcgca
     1021 ggatcgcctg gtaaagatcg accagttcct tcgcttcgct gtcgaagaag tcgatcttca
     1081 agccggcgat gccggcggat tcgcagcggg cgaacagcgc ggcgcgagcc gcggggtcgt
     1141 gcaggtcttt tgagtagacc caggcgaaca tgccgacgtt gcgctgcttg ccgtaatcga
     1201 tgatgccttt cagttccgcg gggctccacc gccgccacat gccttcgacg atctgatatt
     1261 cgaaaccgag ttgatgcgcg ccgtcgaaga aatgcctgat gccctgggcg ctctggtcac
     1321 cgccgtcgag atagcgccag acggcgcggc cggatttcac ccaatcggtc gcggggcctt
     1381 gcggaaagat cgccttatcc ggcggcgggc acaggctcgt gatcgcatcg ctgttgacca
     1441 gcgtgtccaa gtcgctgccc agcaccatca cgcgccaggg cgtgacgatc tcgccatcga
     1501 ccgatgcggc aaccgatagt cgcctggctt cgctggcgcg atagcgcaga cgaaacggcc
     1561 cagagaccgg ctgacgatga ccgagcgcga tggtgaaccc gcgctgcccg tccgcctgaa
     1621 gcgccatgcc cgcatagttc ttcaggtccg cctcggtgat ggcgacgtag ccgtgcttgc
     1681 tcggcaacct aaacgtcacc ggcggcgccg cccattcacc cgcggacacc tgctcgatct
     1741 catgccgcgc gtattcacct tcataatgac cgctgagtcc gtggaaccaa ccgatcgacc
     1801 ccgccggcag cgtgaacgtc gtcgcctcgt ccggcacgcg cgggtgtccg gcatcgggca
     1861 cgatgaaccg aaacgcaatc gcatcatcga acgcgcgcac atcgagcgtg tacctggcgc
     1921 cgctcttgtg ccgcagcgtg atgcgctgtc cgcggcaatt gttgatggcc agcgaatgga
     1981 tgccgaccca cggataggtt tcgtggatct ggtaagagtc gatccgcgtc ggctcggcat
     2041 cggcggtcag atcgacgtcg tccacggtga agaccatcgg gctcgattcg atggccggcg
     2101 ctttcttcaa cgtcaccgcg aacatcaatc gccccgcgtg ctgcgtgaac gtgacggcgg
     2161 tgtcgccgct ttgactggcg acggtggcga agggggcatc ggggtggaat tgaaagttga
     2221 cgggagtggt cgccagcgcc ggcgtggatg aaggagttga cgcagccggc gcgacgctgg
     2281 cgggttcatc gtgatgtgca ggctgacacg ccg
//
LOCUS       DATIMH010001116         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4596716, whole genome shotgun sequence.
ACCESSION   DATIMH010001116 DATIMH010000000
VERSION     DATIMH010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4596716"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VLI90_19255"
     CDS             complement(<1..322)
                     /locus_tag="VLI90_19255"
                     /inference="COORDINATES: protein motif:HMM:NF017973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16410.1"
                     /translation="MSEPIDLFRRVLAGASRAIAKDPDVEVVFASDNAPASGKTARVP
                     SPGAGLEPRLVAEARGAADSAALRLRYHDARLHARNAPMDVEARAVFDALEMARVEAL
                     GARAM"
     gene            528..2120
                     /locus_tag="VLI90_19260"
     CDS             528..2120
                     /locus_tag="VLI90_19260"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HSV16411.1"
                     /translation="MSDVPASLTRIHAALDADGINVDPYFGASIGGKPILPLTGADSV
                     VSHLTYGVTQSAPNGVITYSFMHTPDLTGRYNAPKDQGTASHSDHSAFFPGQDASDGY
                     YAFTPEQEAAARAAIQLWDDLIPQTFKETNGNGADIQFANSNDPGQAYTWPPYWNGHG
                     ATFQGDIFTHDPVTNPSNLQFGFGEYGRTTLIHEIGHSLGLSHPGNYDASDPTGPTYE
                     HDAEYFQDSQEYSIMSYFGAWNTGGAPLDWLHSGGALYDNSPQGPMLHDIVAIQSLYG
                     ADPTTRAGDTTYGFHSNAGNALYDFNQNPLPYYSLYDAGGGNDTIDLSGFHTSQYLNL
                     NPGEFSSIGDVPLTNSEIGQMYRDTYVEYAGYDYFQHGFNATTLGSAVLRSVESGNAT
                     ALASDTGVTGLGTVQYENFSIAYNTIIENAIGGQGRDLLVGNAADNRLDGQGGNDVLT
                     GGAGKDVFVFENNHSTDTITDFQTGVDKIDLSSISGVGASNVSYNATTHQVQIDTDHN
                     GIADLFIISTHVVNSGDYIFHA"
     gene            complement(2173..>2490)
                     /locus_tag="VLI90_19265"
     CDS             complement(2173..>2490)
                     /locus_tag="VLI90_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16412.1"
                     /translation="AAVEAQIVLAKTGEYDEPGGKDTVDKMIKVAELSRQGFINGDIS
                     TVMSPRTVISWAQNAHIFGDLGYAFRVSFLNKCDESERPLIAEYYQRVFGQDLPESVV
                     GKA"
BASE COUNT          509 a          813 c          732 g          436 t
ORIGIN      
        1 ccatcgcgcg ggcgccaagc gcctcgaccc gggccatctc cagcgcgtcg aacaccgccc
       61 gcgcttcgac atccatcggc gcattgcggg cgtgcagccg ggcgtcatgg taccgcagcc
      121 gcagcgcagc cgaatcggca gcgccccgcg cttcggcgac gagccgcggc tcaagccccg
      181 ctccgggcga cggcacgcgc gcggtcttgc cggacgccgg cgcgttgtcg gaggcgaaga
      241 ccacttcgac gtccgggtcc ttggcaatgg ctcgcgacgc acctgccagg acacgccgaa
      301 aaaggtcgat cggttcgctc atcggagcgg atgtaaggcc tgtctcaaaa aggcgcaaag
      361 cccggccagg cttcgccacg tcgcgcagaa acgctggaaa aataaatggt tacccggatg
      421 tgaggaatcc ttgacgaaag ttaactgtca ctccagtgtc cgtgtttcgc cctccaagcg
      481 ggcgtacggc ccatgggttt tggggccgat cacgagggga acagtgcatg agtgacgtac
      541 cagccagtct gacgcgcatc cacgcggcct tggatgctga tggaatcaac gtcgatccgt
      601 acttcggtgc ttcaatcggc ggcaagccga tccttccgct gaccggcgcc gacagtgttg
      661 tcagccacct gacgtacggc gttacgcaga gtgctccgaa tggcgtcatc acctacagct
      721 tcatgcacac gcccgacctc accggccgct acaatgcgcc gaaggatcag ggcaccgcga
      781 gccacagcga tcatagcgca ttctttcccg gccaggatgc atcggacggc tattacgcgt
      841 tcacgccgga gcaggaggca gccgcacgcg cggccattca gctatgggac gacctgatcc
      901 cgcagacctt caaggaaacc aacggcaacg gcgccgacat ccagttcgcg aacagcaacg
      961 atcccggcca ggcttacacg tggccgccat attggaacgg tcatggcgcc acgttccagg
     1021 gcgacatctt cacccacgat ccggtgacga acccgagcaa cctgcaattc gggttcggtg
     1081 aatacggccg caccacgctg atccacgaga tcggccattc gctgggcctc tctcaccccg
     1141 gcaattacga tgccagcgat cccactgggc caacctacga gcacgacgcc gaatatttcc
     1201 aggacagcca ggaatattca atcatgtcct atttcggcgc ctggaacacc ggcggcgcgc
     1261 ctctggactg gctgcattcg ggcggagcgc tttacgacaa cagcccgcag gggccgatgc
     1321 ttcacgacat cgtcgccatc cagagccttt acggtgctga cccgacgacc cgcgcggggg
     1381 acacaaccta cggtttccat tcaaacgccg gcaacgccct gtacgacttc aaccagaacc
     1441 cgttgcctta ctattctctg tatgatgccg gcggcggcaa tgacacgatc gacctgtcag
     1501 gcttccacac gtcgcagtat ctgaacctca atcccgggga gttcagttca atcggcgacg
     1561 tgccgctgac gaacagcgag atcggtcaga tgtaccgtga tacgtatgtc gagtacgcgg
     1621 ggtacgacta tttccagcat ggatttaacg ccacgaccct ggggagcgcg gtgcttcgga
     1681 gcgttgaaag cggcaatgcc acggcgctgg cgtcagacac cggcgtaacg ggcctcggca
     1741 cggtccagta cgagaacttc tcgatcgctt acaacacgat cattgagaat gcgatcggcg
     1801 gacaagggcg cgatctcctg gtcggcaacg ccgccgataa ccgcctggac ggccagggtg
     1861 gcaacgacgt gctgaccggc ggcgccggca aggacgtgtt cgtgttcgag aacaatcatt
     1921 ccaccgacac gatcaccgac ttccagaccg gcgtcgacaa gatcgacctc agcagcatct
     1981 cgggcgtggg ggcgtcgaac gtttcgtaca acgcgacgac tcaccaggtt cagatcgaca
     2041 cagaccacaa cggtatcgcc gacctgttca tcatttcgac gcacgtcgtg aacagcggcg
     2101 actatatttt ccacgcctga tcaggcgacg atcgacaact aggaagggcg tcctgcgggg
     2161 cgcccttttc tgtcatgctt tgccgacgac gctctcgggc aaatcctggc cgaagacgcg
     2221 ctggtaatat tccgcgatca gcgggcgctc ggactcgtcg cacttgttga ggaagctgac
     2281 ccggaaggca tagccgagat cgccaaagat atgcgcgttc tgcgcccagc tgatcaccgt
     2341 ccgcgggctc atcaccgtgg agatgtcgcc gttgatgaac ccctggcgcg acagctcggc
     2401 gaccttgatc atcttgtcga cggtgtcctt gccgccgggc tcgtcatatt cgccggtctt
     2461 ggcgagaacg atctgcgcct cgacggccgc
//
LOCUS       DATIMH010001117         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3154181, whole genome shotgun sequence.
ACCESSION   DATIMH010001117 DATIMH010000000
VERSION     DATIMH010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3154181"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VLI90_19270"
     CDS             <1..367
                     /locus_tag="VLI90_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16413.1"
                     /translation="RVPLALFDARMKSANASPMLGHITAFQRKLLKFDNDPVTDDRLS
                     DGLVLVSFEKIAGQDRYGMIFRKVGDKWWIDDIPNMFPAERAGQGVPGGGGGGAPSAA
                     PAKPTQVPSGPAGPPAPPK"
     gene            665..1864
                     /locus_tag="VLI90_19275"
     CDS             665..1864
                     /locus_tag="VLI90_19275"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HSV16414.1"
                     /translation="MAMTLLNKRRIVKWLLSGAAFAAVVASSGCSLLHKNQPAATAAS
                     APVANATPSAPQDDTDAFEAPAPPPQVNIFGELDGVARGPVKPIGDEGFQQHTFADEG
                     YDSDVSIDPAGKLLVFASTRHSEHAGIYLQRVEGTAVTKLTADDADNAYPTFSPDGKS
                     IAFCSTRSGVWNIYVMDTDGRNVVTITSGSAQCVHPSFSPDGGRLVFSEMGSRSNQWE
                     LWTVNLSSGEKRQIGYGVFPNWSPSKDVDRIAFQRARQRGSRWFSLWTLDLVDGEARR
                     VTEVAVSSNAAIVSPAWSPDGKRLVFATIIDPTRTAGRRKGEQDIWTINADGSDRRRL
                     TDGNGVNLSPVWGVDNRVYYISDRSGTESIWSVRPEMGQAFTAAAPKTQKTNETVGAA
                     DTRDVGN"
     gene            1980..2750
                     /locus_tag="VLI90_19280"
     CDS             1980..2750
                     /locus_tag="VLI90_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16415.1"
                     /translation="MRTMRSITHNVLLLALVAGCQTPQYGKEEQLTLQSRTQPIWAVA
                     PAVNLSGEPSVDPLLQADLVYQQIQAVHNVTVVPVNRVIEVYAALRIEKVESEEQAAI
                     VCEQLGCDALLIPTVTAFDPYNPPKVGAAVQLITRPGSVYRRNVNARELSRRATPGFD
                     ESLPPHPNFIQVVGMYDSANGSVRDAVLNYAKGRNDPVGPLGSKEYFVNMDRYCGFVY
                     HSLIADLIREAAKREADDRADSNHPAVSQRATLTAPQG"
BASE COUNT          583 a          880 c         1004 g          497 t
ORIGIN      
        1 gcgcgtgccg ttggcgctgt tcgatgcgcg gatgaagtcg gcgaacgcgt cgccgatgct
       61 cgggcacatc acggcctttc agcgcaagct gctgaagttc gacaacgatc cggtgacgga
      121 cgatcgcctg agtgacgggc tggtgctggt gagcttcgag aagatcgccg gtcaggaccg
      181 gtacggcatg atcttccgca aggttggcga caagtggtgg atcgacgaca tcccgaacat
      241 gttcccggcc gagcgcgccg gccaaggggt gccgggcggt ggtgggggag gcgcgccaag
      301 cgctgcgccg gcgaaaccga cgcaagttcc gtccggtccc gccggaccac cggcgcctcc
      361 aaaataatct tcacatcgtg acaaatcgca gccgcgaggc tttagcccgc ggaccgttgg
      421 gatatccggc ggggattcga ctgcgaagcg tccgcgggct aaagcctcgc ggctgcgatt
      481 tctatcgcat tcgccgcgca tccgtctcac cttcccattc aagcaaccct ccccccacgt
      541 ccgataatca tcgcatgtgc ctccgtgcgg cgctgcgccc tcagaacgac ccccgattcc
      601 gccgatacgg ctttggggaa ctcgtgacgg ttatgcagat gcgtaagcgc gaggcacgtg
      661 cgacatggcg atgaccctgc tgaacaaacg gcgaatcgtg aaatggctgc tgagcggagc
      721 cgcgttcgcg gccgtcgtgg cttcgtccgg ctgctcgctg ctgcacaaga atcagccggc
      781 ggcgacggcg gcgagcgcgc cggtagcgaa cgcgacaccg agcgcgccgc aggatgacac
      841 cgacgcgttc gaagcgccgg ctccgccgcc gcaggtgaat atatttggcg agctggatgg
      901 cgtggcgcgc gggccggtga agccgatcgg cgacgaagga tttcagcagc acacgttcgc
      961 ggatgaagga tacgactccg acgtctcgat cgatccggcc ggaaagctgc tggtcttcgc
     1021 cagcacgcgg cacagcgaac atgcggggat ttacctgcag cgcgtggagg gcaccgccgt
     1081 cacaaagctg acggcggacg acgccgacaa cgcgtatccg acgttcagcc ccgacggcaa
     1141 gtcgatcgcg ttttgctcga cgcgcagcgg cgtttggaat atctacgtga tggacaccga
     1201 cgggcgaaac gtcgtcacca tcaccagcgg ctcggcgcag tgcgtgcatc cgagcttcag
     1261 cccagatggt ggccggctgg tgttcagcga gatggggtcg cgcagcaatc agtgggaact
     1321 gtggaccgtg aacttgagca gcggcgagaa gcggcagatc ggttacggtg tgttccccaa
     1381 ctggtcgccg agcaaagacg tcgatcgcat cgcgtttcaa cgcgcccgcc aacgcgggtc
     1441 gcgctggttc agcttgtgga cgctggatct ggttgatggc gaagcgcggc gggtgacgga
     1501 agtcgcggtg agttcgaacg cggcgatcgt ctcgccggcg tggagcccgg atggaaagcg
     1561 actggttttc gcaacgatca tcgacccgac gcgcaccgcg gggcggcgca agggtgagca
     1621 ggacatctgg acgatcaacg ccgacggcag cgaccgccgg cggttgaccg acggaaacgg
     1681 tgtaaacctg tcgccggtgt ggggcgtgga caatcgcgtt tactacatca gcgaccgcag
     1741 cgggaccgag tcgatctggt cggtgcgccc ggagatgggg caggcgttca ccgcggcggc
     1801 gccgaagacg cagaagacga atgagacggt cggcgcggcg gacacgcgcg acgtggggaa
     1861 ctgatccacg ccgggttcgg aggtactccg acgacccgga tgtattgcgt aaatcgaaaa
     1921 gaatccgggt cgtcggagta cctccgaacc cggcgtgaag agtgtttcaa ggacgaaaca
     1981 tgcgaacgat gcggagcatc acacataacg ttctcctgct cgcgctggtc gcgggttgcc
     2041 aaaccccgca gtacggcaag gaagaacaac tcaccctgca gtcgcgcacc cagccgatct
     2101 gggcggtcgc gccggcggtg aacctcagcg gcgagcccag cgtcgatccg ctgctgcagg
     2161 cggacctggt ctatcagcag atccaggccg tgcacaacgt cacggtcgtg ccggtcaatc
     2221 gcgtgatcga agtatacgcg gcccttcgga tcgagaaggt ggagagcgaa gagcaggcgg
     2281 cgatcgtctg cgagcagctg ggttgcgatg cgctgctgat ccccacggtc accgcgttcg
     2341 atccgtacaa cccgccgaag gtgggagcgg ccgtgcagtt gatcacccgg ccgggcagcg
     2401 tgtatcgcag gaatgtgaat gcgcgcgagc tgtcgcgccg cgccacaccg gggtttgacg
     2461 aatccttgcc gccgcatccg aattttatcc aggtggtggg gatgtacgat tcagcgaacg
     2521 gaagcgttcg cgacgccgta ttgaattatg caaagggccg gaacgacccg gtggggccgc
     2581 tgggcagcaa agaatacttc gtgaatatgg accgttattg cgggttcgta tatcacagtt
     2641 tgatcgcgga cctgatccgc gaagcggcca aacgtgaggc ggatgaccgg gcggattcca
     2701 atcacccggc cgtcagccaa cgggccacgt taaccgcgcc tcaaggctga ccgatcaaga
     2761 ccaatgggca ttggcgttat cggtatgctg tccccgctgc acatggatgt gcgcggccgg
     2821 aagaatgggt ctgcgaatta cgggttcatc gaaggctcgc acgtttcact cgtttcaaat
     2881 gaagcgcggc gcgcgttcga tgcgggagtt cacaagatgt cggctgtcaa aacgctgagc
     2941 ctcgtcaagg gcaacgaaca cttt
//
LOCUS       DATIMH010001118         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3154248, whole genome shotgun sequence.
ACCESSION   DATIMH010001118 DATIMH010000000
VERSION     DATIMH010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3154248"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VLI90_19285"
     CDS             <1..189
                     /locus_tag="VLI90_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16416.1"
                     /translation="IRVVIGPGEDVSGRVRDQDKYALRVTRYESRLYARIHVITQSRI
                     CLPTFPIVHQVKSGKTQL"
     gene            complement(337..2604)
                     /locus_tag="VLI90_19290"
     CDS             complement(337..2604)
                     /locus_tag="VLI90_19290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV16417.1"
                     /translation="MHRSALDELEVAPAMQSRPAPRAPRAQRFATPLILLAITVFAGV
                     IRFSHLERPPLLIDECFTFWRTCGSYGQLVETLRDDGFVPLHYELLWWIKQGMPLGPH
                     VHIVRDGLFQTPFIMRFIPALCGTLMVPVMYFVARQLFGRATSLLAAFFTACSAYAMF
                     FSRDGKMYAPAWMLAALAVGSLLWWLRTRRSTAWLAWVAAGSAAAGVHVVTMLLLVLA
                     PLFLLTQRRMHWRQGLLMLAGAMLIAAGPAGYYLTFNRWAERSGGLVPGVTAEPAPDA
                     NWGASGLGWIGDREIGANEAFRSLSAYLTAYEWDGHHSFLNWGYRPNWVSRTGIACVS
                     VICGLLVLGALPWPRRWRLYRGVNFDAPEPSWRAALWLACWIVLPAYGFFYCRSYDDA
                     ASPGDWLAMAGAWWGERWWWIVLATVALAGVATRWRRAGDVLGGIVVLAVVTIIVLFA
                     IDKADDVRMVDWLGASLRDAALIFVPAALWMFSGNSNRERLAAALRFAIVLGVVLSLC
                     AGAHATWDSLRAASMARHPEIVWHSIWHTRYIGVVLPAVWIAAAALVMRLPTRPLRIA
                     AVILIIAPNLANGIARFNAQTQVPYDRVFIDVWLAKPGSNVRTYFDLEGQVFGASGFP
                     KQAYWRPEAAYNAALAGRLATTPPEFRAGKDWPFVYGPFVERFAKWVEYRPYTSAASI
                     HSDLNPTVDRVIVWNIDRMFQTTAADYQRQLGPTWRTTSDESIGVWWFWNWTQQLSFH
                     RCEFTRVAPAATTQP"
     gene            complement(2672..>3006)
                     /locus_tag="VLI90_19295"
     CDS             complement(2672..>3006)
                     /locus_tag="VLI90_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16418.1"
                     /translation="VDVLLDGGRVEIWDSHAGAPPRTQIHRWQIFWGFRLRLPDVRRS
                     AWEFDAHALGNAKLGRLYLIAFPIWCALLPCLIAPALWWRDHRRRGRAEQRGFPLDAV
                     ALAATSPA"
BASE COUNT          618 a         1069 c          889 g          430 t
ORIGIN      
        1 atccgtgtgg ttattggacc gggtgaagac gtaagtgggc gagtgcgcga tcaggataaa
       61 tacgcattac gagttacgcg ttacgaatca cgcctctatg cacgcattca cgtcatcacg
      121 caatcacgca tttgcctccc caccttccca atcgtacatc aagtgaagtc aggcaagacg
      181 caactttgat gcggcacgcg tgacgaacgc gtgtgacgtc gaagtgcctg acgcgcggga
      241 atcggcggat gccgatcggc gcaaatgcgc gatgctcccc tccctctttt ctggcgatgc
      301 cggccacggc acaaccgtgg ccggcatttt ttttgatcag ggttgtgtcg tcgccgccgg
      361 cgcgacgcgc gtgaactcgc agcggtggaa cgacagctgc tgcgtccagt tccagaacca
      421 ccagacgccg atcgattcat cgctcgtcgt tcgccaagtc ggcccgagct gacgctgata
      481 atccgccgcc gtcgtctgaa acatgcgatc gatgttccac acgatcacgc gatcgaccgt
      541 cgggttgaga tcactgtgta tcgacgccgc cgacgtgtaa ggccgatact ccacccactt
      601 cgcaaagcgc tcgacgaacg gcccatacac gaacggccaa tctttgccgg cacgaaactc
      661 cggcggcgtg gtcgccagcc gccccgccag cgccgcgttg tacgccgcct ctggccgcca
      721 atacgcttgc ttcggaaagc cggacgcgcc gaagacctgt ccttccagat cgaaataggt
      781 ccgcacgttc gaacccggtt tggccaacca cacatcgatg aatacccggt catacggcac
      841 ctgcgtctgc gcgttgaagc gcgcgatgcc gttcgccagg ttcggtgcga tgatcaggat
      901 gaccgcggcg attcgcagcg gccgggtcgg cagccgcatc accagggccg ccgccgcgat
      961 ccacaccgca ggcaggacca cgccgatgta ccgcgtgtgc cagatcgagt gccagacgat
     1021 ctccggatgc cgagccatcg acgcggcgcg cagcgaatcc cacgtagcat gcgcgcccgc
     1081 gcatagcgat aaaaccacgc cgagtacgat tgcaaaccgc agcgccgccg cgaggcgctc
     1141 gcgattgctg ttgccgctga acatccacag cgccgccggc acgaagatga gcgcggcgtc
     1201 ccgcaacgag gctccaagcc agtcgaccat ccgcacgtca tccgccttgt cgatcgcgaa
     1261 cagcacgata atcgtaacca ccgccagcac cacgatgccg ccgagcacgt caccggcccg
     1321 gcgccaccgc gtcgccacgc cggccagcgc caccgtcgcc agcacgatcc accaccatcg
     1381 ctcgccccac catgcgccag ccatcgcgag ccaatcgcca ggcgacgccg cgtcgtcgta
     1441 cgaccggcag tagaagaacc cgtacgccgg cagcacgatc cagcacgcca gccacaatgc
     1501 cgcccgccag ctcggttcag gcgcatcgaa gttcacaccg cgatacaacc gccaacgccg
     1561 cggccacggc agcgcgccta gcaccagcaa tccgcagatg acgctgacgc acgcgatgcc
     1621 cgtgcgcgag acccagttcg gccgatagcc ccagttcaaa aagctatgat ggccgtccca
     1681 ctcgtacgcg gtgagatacg ccgacagcga tcggaacgct tcgtttgcgc cgatctcgcg
     1741 atcgccgatc caacccaggc cactcgcgcc ccaattggca tcgggcgccg gctcggcggt
     1801 cacgcccggc accagcccgc ccgagcgctc ggcccaccgg ttgaacgtca ggtaatatcc
     1861 cgccggtccc gcggcgatca gcatcgcgcc ggccagcatg agcaatccct gccgccagtg
     1921 catgcggcgc tgggtcagca gaaaaagcgg cgcgagcacg agcagcagca tggtgacgac
     1981 gtgcaccccc gccgccgccg accccgccgc cacccacgcc agccacgccg tcgatcgccg
     2041 cgtgcgcagc caccacagca gagatcccac cgccagcgcc gccagcatcc acgccggcgc
     2101 gtacatcttg ccgtcgcgcg aaaagaacat cgcatacgcc gagcatgcgg tgaagaatgc
     2161 cgccagcagc gacgttgcac gcccgaacaa ctgccgcgcg acgaagtaca tgaccggcac
     2221 catcagcgtc ccgcacaacg ccgggatgaa tcgcatgatg aacggtgttt gaaacagccc
     2281 gtcgcgcacg atgtgcacgt gcggccccag cggcatgccc tgcttgatcc accacagcag
     2341 ctcgtaatgc agcgggacaa agccgtcgtc gcgcaacgtt tcgacgagtt ggccgtacga
     2401 gccgcaggtt cgccagaagg tgaagcattc gtcgatgagc agcggcggcc gctcgaggtg
     2461 cgaaaagcga atgacgccgg cgaagacggt gatggcaaga aggatgagcg gcgttgcgaa
     2521 acgctgtgcg cgcggcgcac gcggcgccgg ccgcgactgc atcgcgggcg cgacttcgag
     2581 ttcgtctaaa gcgctacgat gcatggcgag gcggtcgaac gatgtgataa ccagcggcga
     2641 tgaaaagcac cagtgcgtcg cgctcctacc gctacgcggg ggatgtcgcg gcgagcgcga
     2701 ccgcgtcgag cgggaacccg cgctgctccg cgcgcccgcg gcgacggtgg tcacgccacc
     2761 acagcgccgg cgcgatcaag cacggtagca gcgcgcacca gatggggaac gcgatcagat
     2821 atagtcgccc caatttcgca ttgcccaatg cgtgcgcgtc aaactcccac gccgatcgac
     2881 gaacgtccgg cagccgcaag cgaaatcccc aaaaaatctg ccagcgatga atctgcgtcc
     2941 gcggcggtgc acctgcgtgg ctgtcccaga tctccacgcg acctccatcc agcaggacat
     3001 caactt
//
LOCUS       DATIMH010001119         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4889812, whole genome shotgun sequence.
ACCESSION   DATIMH010001119 DATIMH010000000
VERSION     DATIMH010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4889812"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..146)
                     /locus_tag="VLI90_19300"
     tRNA            complement(72..146)
                     /locus_tag="VLI90_19300"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Gly,seq:ccc)
     gene            complement(212..772)
                     /locus_tag="VLI90_19305"
     CDS             complement(212..772)
                     /locus_tag="VLI90_19305"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HSV16419.1"
                     /translation="MFGDIARFLLNTVFTLFGAALLLRAWMQVVRMPPYNPVSNAVMQ
                     ATNWIVLPLRKILPGGKIDWASILAAFIAALVYVTLMVYLAGVDPTVMMPMLLLVAVL
                     TVVKWALNLIIWMTILMALLSWLNPQSPAMPLLFQITAPFLDPLRRILPRLGGIDLSP
                     ILLFVIVQVLLMVVTRVAVSMTLFGI"
     gene            1514..1675
                     /locus_tag="VLI90_19310"
     CDS             1514..1675
                     /locus_tag="VLI90_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16420.1"
                     /translation="MSIFSYRKHLRFLSQAQRRRWWDQKKRLTPRVVIGGAYVPPNVS
                     REFAYVKVG"
     gene            2445..>3366
                     /locus_tag="VLI90_19315"
     CDS             2445..>3366
                     /locus_tag="VLI90_19315"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HSV16421.1"
                     /translation="MLSLPVPTFEDVADAARRIDGVAHRTPVLTSRTADVIAGASLFF
                     KCENFQRMGAFKFRGAYNAISHFDDRQRAAGVITYSSGNHAQAIALSARLAGIRATII
                     MPEDAPAAKMEATRSYGGEVITYDRYTQNREEIGRALAEERGMTLIPPHDHPHVIAGQ
                     GTAAKELIEESGPLDYLFVCLGGGGLIAGCALAAAALSPSCMVIGVEPEAGNDAQQSL
                     ARGEIVHIDVPRTIADGAASTHVGEYNFPIIQRHVDRIVTVSDAQLIDTLRFFAQRMK
                     MVVEPTGCLAAAAVLQKIVPVEGKRVGVIVS"
BASE COUNT          674 a          985 c         1077 g          630 t
ORIGIN      
        1 aatgcggaga aacgtcgcgc agaaagtgaa aagccgcctc ggggtatcga aggcggcttt
       61 cgctgtgatt ctggagcggg cgatgggaat cgaacccacg tctgtagctt gggaagctac
      121 ggtaatggcc attatacgac gcccgcgtga gtcggctatt gtaagcgcaa cccgcgccgc
      181 cgcgcaattg cagcgggcgg cgcgctcacg ttcagatgcc gaacagcgtc atcgagacgg
      241 cgacgcgcgt cacgaccatc agcagcacct gcacgattac gaagagcagg atcggcgaca
      301 gatcgatacc gcccaggcgc ggcaggatgc ggcgcagcgg atcgagaaac ggcgcggtga
      361 tctgaaagag cagcggcatc gcgggcgatt gcgggttcag ccacgagagc agcgccatca
      421 ggatcgtcat ccagatgatg aggttcagcg cccatttgac gaccgtcagc accgcgacca
      481 gcagcagcat tggcatcatc accgtcgggt cgacgccggc aaggtacacc atcagcgtca
      541 cataaacgag cgccgcgatg aacgcggcca ggatgcttgc ccagtcgatc ttcccgcccg
      601 gaagaatctt gcgcagcggc agcacgatcc agttggtcgc ctgcatcacg gcattcgaaa
      661 ccgggttgta cggcggcatg cgcacgacct gcatccaggc gcgcagcagc agcgccgcgc
      721 cgaacagggt gaaaacggta ttgagcagaa aacgggcgat atcgccgaac atctcgggtc
      781 ctcttggaaa cgtgcgcagc acagtggctg caccggggtc gggaaaagcg aagggaacgg
      841 gcgatgacgc taacagcctc tatacggccg tggagcgtgc ggcgacgcgg ccatggcgga
      901 agcgccgccg cgtgaatggt atctgctgac cttgatcatt gtttgttctc tggactgatt
      961 gtcgtgccaa agtcatcgaa acagggcgag gcgccgattg cagcgtcgcc actgtttctt
     1021 ttacggcggc cggcgacttg cattccccgc ccacggtgga agccggtcgg gtgccggacg
     1081 tcaccttaac atggctttaa gactgcttgg gggcgttgtc gaacaaggag gagggattgt
     1141 cgcccgggcc aattgaaagc gagccgcaag gcgtggtccg cgcgcgttgg catcgccgga
     1201 aaaccctgat gttgcctgcc ggcgacgctc tcggcgagca gattgaacgc aaagatggca
     1261 ggataggatt tcggaaaacg agcgaacgag ggttgtatta ggatccggcg gcggcgagcc
     1321 gttcaatcca tggcatgttt tctgctgaaa tgtggttcga cacacgtgga tcgcgtggtt
     1381 tggttccttt gcgaacatta acgaacgaac acggcgcgaa tactaaagag aagacagatt
     1441 caacaggaat ggcattgcgc gtcgagcgca cgctttcccg aagggtttcc aatacgctac
     1501 gttcggaggt cgcatgagca ttttctctta tcgaaagcat ctgcggttct tgagccaggc
     1561 ccagcggcgt cgctggtggg atcagaagaa gcgattgacg ccgcgtgtgg taatcggcgg
     1621 cgcctacgtt ccacccaatg tttcgcgcga gttcgcttac gtgaaggtcg gctgacgccg
     1681 ccggacagaa gtcattgtta ttcgttaaga gccccgccgg acgttatgtc cggcggggct
     1741 tttggtttct tttgtaaaga gacgttaccc cggtcaccag ctaagtcgca cgataagaag
     1801 acgcccggcg tgccggttgg cctgccccgc gggtggggcg atccaggcgt acgaatctcg
     1861 ccgcgaagaa cttgcaattt gcaacaaatg gcggcagcgc gcgagcgcgt ttttcgctaa
     1921 attattctta cgccgcggtt gaccggatag accggccgaa agcggcgagg agaacgaaag
     1981 tgaaaacgct cggtagtttg ttgtttgctg gagcgctcgg tctgggtatc gccagcgggg
     2041 cgcaagcgca tgtttcaatc ggggttggta tcggagtgcc tgcatatcct gcgtatcagg
     2101 cgccgcccgc gccggtttac atggcgccgc cgcagccggt atatgtcgcg cctccgccag
     2161 tcgtttatgc accggcgccc gccgtagtcg tcggcggata taactacgga tactacggtc
     2221 ggccatattg gcgccacggg tattacggcc atccctatgg tcatggcggt tatggtcacg
     2281 gtggctatga ccactggcgc agataatcgc tgtccggaag aatgcttgat gcaagcccct
     2341 ttttaggggc ttgtcttttt taccgcgcca aaatgtgtgc ggactatttc gattcccgtc
     2401 tgacctgcga gaatatcctc ctcccgacga cactcccgac accgatgctc tcacttcctg
     2461 tcccgacttt cgaagacgtc gccgacgccg cccggcgcat cgacggcgtt gcgcatcgca
     2521 cgcccgttct cacatcccgc acggcggacg taatagcggg cgcgtcgctc ttcttcaagt
     2581 gcgaaaactt ccagcgcatg ggcgcgttca agtttcgcgg cgcatataac gcgatctccc
     2641 acttcgacga ccgccagcgc gccgctggcg tcatcacgta ctcgtcgggc aatcacgcgc
     2701 aagcgatcgc gctgtcggcg aggctcgcgg gcatccgcgc aacgatcatc atgcccgagg
     2761 acgcgcctgc cgcgaagatg gaagcgacgc gcagctacgg cggcgaagtc atcacttacg
     2821 accgctatac gcagaatcgc gaggaaatcg gccgcgcgct tgccgaagag cgcggcatga
     2881 cgctcattcc gcctcacgac catccgcacg tgatcgcggg gcagggcacg gcggcgaagg
     2941 agctcatcga ggaaagcggg ccgctcgatt acctcttcgt atgtctcggc ggcggcgggc
     3001 tgattgccgg atgcgcgctc gccgcggcgg cgctgtcgcc gtcgtgcatg gtgatcgggg
     3061 tggaaccgga agcgggcaac gacgcgcagc agtcgctcgc gcgcggcgag atcgtgcata
     3121 tcgacgttcc gcgcaccatc gccgatggcg cagcatccac gcacgtcggc gaatacaact
     3181 ttccgatcat ccagaggcac gtggatcgca ttgtcaccgt cagcgacgcg caattgatcg
     3241 acacgctgcg cttttttgcc cagcgcatga agatggtcgt cgaaccgacc ggctgcctcg
     3301 cggcggcggc ggtgcttcag aagatcgtgc cggtggaggg caagcgcgtc ggcgtgatcg
     3361 tgagcg
//
LOCUS       DATIMH010001120         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_556366, whole genome shotgun sequence.
ACCESSION   DATIMH010001120 DATIMH010000000
VERSION     DATIMH010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556366"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VLI90_19320"
     CDS             <1..759
                     /locus_tag="VLI90_19320"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HSV16422.1"
                     /translation="LVVNGDDAELLDAVSTYPGKRVTFGFKETNDLWPADIHCSEQGI
                     RYRLNGSRQEVFVPMLGRHTACNSLAAIAVARRMGVSDKLIYESLAEATGPEWRLQLQ
                     HVGKICVLNDAYNANPNSMRAALETVRDLQVKGRRLAILGDMKELGEAADRYHREIGA
                     FAATCKFDSLYCVGPKSALIADAAEAAGMDKRKVVHFTSAADCAQIAPRWLRRGDMVL
                     LKASRGVRLETVAAAIVEAFGGETKHAATRKIAS"
     gene            914..>1787
                     /locus_tag="VLI90_19325"
     CDS             914..>1787
                     /locus_tag="VLI90_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16423.1"
                     /translation="MRDFLICLCLTAATLAVYWPVDRFGFVHFDDDRYVYENPHVLGG
                     LTPENIAWAWRSPQIANYHPMTLMSYQLDATLFGARAGRYHVENVLLHILATLLLFNV
                     WRAATGDTWPPAFVAAVFALHPAHVESVAWVSERKDVLSVLFMLLAMRSYIAFARAEL
                     EGPRRTVVYAGTMTAFALGLLAKSMIVTLPVLLLLMDDWPLRRLQRARWTSAITEKVP
                     MLILSLAAGAMAMRAQRAAGVVADLAAVPLRYRLASSVVSYARYLGKTFWPTNLAPFY
                     PYRSDWSPLQIGASL"
BASE COUNT          303 a          563 c          580 g          341 t
ORIGIN      
        1 ctggtcgtca acggcgacga cgcggaattg cttgacgccg tctcgactta tcccggcaag
       61 cgcgtcacgt tcggctttaa ggaaaccaac gacctttggc cggccgacat ccattgcagc
      121 gagcagggca ttcgctatcg cctgaacggc agccggcagg aagtgttcgt gccgatgctc
      181 ggccggcaca ccgcatgcaa ttcgctggcg gccatcgcgg tcgcacggcg catgggcgtt
      241 agcgacaagt tgatctacga atctttagcc gaagcgaccg gtcccgaatg gcggctgcag
      301 ctgcagcacg tcggcaagat ctgcgtgctg aacgatgcgt acaacgccaa ccccaattcg
      361 atgcgagccg cgctggaaac cgtgcgagac ctgcaagtga agggccggcg attggcgatc
      421 cttggcgaca tgaaggaact cggcgaagcg gcggatcgat atcaccgcga gatcggcgcc
      481 ttcgccgcca cgtgcaagtt cgattcgctg tattgcgttg gacccaagag cgcgctgatc
      541 gccgacgccg ccgaggcggc ggggatggac aagcggaagg tcgttcactt caccagcgcc
      601 gccgactgcg cgcagatcgc cccgcgctgg ctccgccgcg gggacatggt gctgctgaag
      661 gcatcgcgcg gcgtccgcct cgaaacagtc gccgccgcca tcgtcgaggc atttggcgga
      721 gagacaaagc acgctgcgac gcggaagatc gcgtcgtaga acacgtctcc ttcaccatcc
      781 acctttagcg gctgcgtttt gtgcggcccg tcttcttctc cgaacggtgt aactcgacaa
      841 aggcttgccg cgtaagcgcg gccgctaaac gtgtggtgcg cgcacgcgca tccggtatga
      901 tcgcgcgcgg ctcatgcgcg acttcctcat ctgcctctgt ctcaccgcgg ccacgctcgc
      961 tgtgtattgg ccggttgacc gttttggctt cgtccacttc gatgacgatc gctacgttta
     1021 cgaaaatccg cacgtccttg gcggattgac gcccgagaac atcgcgtggg cgtggcgttc
     1081 gccgcagatc gcgaattatc acccgatgac gctgatgtcg tatcagctgg atgccacgct
     1141 cttcggcgcg cgagcgggac ggtaccacgt tgagaacgtc ctgctgcaca tcctggcgac
     1201 gctgctactg ttcaacgtct ggcgggctgc gacgggcgac acgtggccgc ccgcgttcgt
     1261 cgcggcggtc ttcgcgctgc atccggccca cgtggaatcg gtggcgtggg tgtccgaacg
     1321 caaggatgtt ctgagcgtcc tgttcatgct gctggcgatg cggagctaca tcgcgttcgc
     1381 ccgagcagag ctggagggac cgcgacgaac ggtggtatat gccggcacga tgaccgcatt
     1441 cgcgctgggc ttgctcgcca agtcgatgat tgtgacgttg ccggtgcttc tgctgctgat
     1501 ggacgattgg ccgctgcggc ggctgcagcg ggcgcgatgg accagcgcga tcaccgaaaa
     1561 ggtgccgatg ctgatcctgt cactggccgc cggcgcgatg gcgatgcgcg cccagcgtgc
     1621 cgcgggcgtc gtcgcggact tggccgccgt gccactgcgg tatcggctgg caagttccgt
     1681 cgtctcatac gcgcgctacc tgggcaaaac attttggccg acaaacctgg cgccgttcta
     1741 tccgtatcga tccgattggt cgccgctgca aatcggcgcg agcctcg
//
LOCUS       DATIMH010001121         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3154783, whole genome shotgun sequence.
ACCESSION   DATIMH010001121 DATIMH010000000
VERSION     DATIMH010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3154783"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VLI90_19330"
     CDS             complement(<1..389)
                     /locus_tag="VLI90_19330"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSV16424.1"
                     /translation="MPEPLKMIYEKDAPIDALKGKTVAILGFGSQGHAHGLNLRESGV
                     KVIVANRKDSANGRLAIEHGFDPVSVEDATKAADLIIVTLPDEVQPDVWKKSVAPNLK
                     AGTAIGTTHGFNIHFKTILPPKENDVLL"
     gene            730..1980
                     /locus_tag="VLI90_19335"
     CDS             730..1980
                     /locus_tag="VLI90_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16425.1"
                     /translation="MSRVTARAALLFIAAVATGADQPASDDPRRAQVQAAATNAAVNL
                     REQVLRMPLTRGIDVRAVVDRTQTTAALDQALQGAQQIGGPRWIDDRTCQVRLEIPGS
                     QLSGVLLNEVRQHADQSPLPIDELQRRLHDWDRITFYAVGSSAAGGEAIEMARPHVAV
                     GAWAKVDEATRKRTITDARQDAVRHVLDDVAPVALSETVQVRDAIAVPAIGDRMNVWL
                     NHQPVTRLEFLEDLKVSVTISPSPKAMTSTLQSAVTSDKEFSKNRTFNWGQVQQDVAT
                     RVGPTVGTAAAQPAPPAPAPQAPGNGLLPAMVLPLQPPDWIDSPIDAEATSKSVGTRL
                     KTAQVAEAAATEQLRRKFLELHLTPQMTLGDAVKTDGRLGDAVDRAMLHARIVKVDYR
                     PDGSVNARVRLDTQSAWDELRSNP"
     gene            2391..2855
                     /locus_tag="VLI90_19340"
     CDS             2391..2855
                     /locus_tag="VLI90_19340"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSV16426.1"
                     /translation="MFRSMKWIAPVAGLALFLGFNQVTKAADEAPAAGKATVTVTVQD
                     KDGKGVEGVRVRLVSATPPKTEGGAEAKAAGDGGKKGGGKMASVAQGTTDASGKAVLA
                     NVPDGEYTVMASLKGGGAGRETVKVEGGKDVAVTVTLKDKKPKAEAPPAEAK"
BASE COUNT          569 a          925 c          980 g          501 t
ORIGIN      
        1 agcagcacgt cgttttcctt cggcggcaag atcgtcttaa aatggatgtt gaagccgtgc
       61 gtcgtcccga tggccgtgcc ggctttcagg ttcggcgcca cgctcttctt ccacacatcc
      121 ggctgcactt cgtcgggcag cgtgactatg atcaggtccg ccgccttggt cgcgtcttca
      181 accgagacgg gatcgaaccc atgctcgatc gcgagccggc cgttggccga gtccttgcgg
      241 ttggcgacga tgaccttgac gccgctctcg cgcaggttga gcccgtgggc gtgcccctgc
      301 gaaccgaagc cgaggattgc tacggtcttg cccttgagcg catcgatggg ggcgtccttc
      361 tcataaatca tcttcaacgg ctcgggcatg acgtactcct ccgggcgaaa actcagctgg
      421 aaaattcgag ccgcggatgg tagcggcgcg gggtggggga tgtcaaaagg atgcaggcgc
      481 gccagtgccc tgcgtagcgg tggcccgaag gggcatgtgt tttcctcctg aagtgaaaga
      541 cgtgcccctt aggggcgacc gctacgcacg cacgcacgca cgctcccgca cgcataccac
      601 gatcatacgt gccatttgac gcggctgacc ctaacttggc cccgctccaa ctgtctggtg
      661 taatggggag tcagttgacg ataatgtccg ataatatcga tgcgtagaac aaattctgga
      721 tcatcacgat tgagccgcgt cacagcgcgg gcggcgttgc tgttcatcgc cgccgtcgca
      781 accggtgcgg atcagcctgc cagtgatgac ccgcgccgcg cccaggtcca ggccgccgcc
      841 accaatgccg cggtcaacct gcgcgagcag gtgctgcgca tgccgctgac ccgcggcatc
      901 gacgttcgcg ccgttgtcga tcgcacgcag accactgccg cgctcgacca agcattgcaa
      961 ggcgcgcagc agatcggcgg cccacgttgg atcgacgacc gcacctgcca ggtccgcctg
     1021 gagatccccg gttcacaact ctccggtgtc ttgctgaacg aggtgcgcca gcacgcggac
     1081 caaagtccgc tgccgatcga cgagcttcag cgccgcctgc acgactggga tcgcatcacg
     1141 ttctacgccg tcggttccag cgccgccggt ggcgaggcga tcgagatggc ccgcccgcac
     1201 gtggccgtcg gcgcctgggc gaaggtggat gaggcgacgc gcaagcgcac gatcaccgac
     1261 gcccgccagg acgcggtgcg gcatgtgctg gacgacgtcg cgcccgtcgc cctcagcgaa
     1321 acagttcagg tgcgcgacgc catcgccgtg ccggcgatcg gcgatcgcat gaacgtctgg
     1381 ctcaaccatc aaccggtcac gcggctggag ttcctggaag acctgaaagt gtcggtgacg
     1441 atcagcccat cgccgaaggc gatgacctcg acgctgcagt cggccgtcac cagcgataaa
     1501 gagttttcga agaaccgcac gttcaactgg ggtcaggtgc agcaggacgt ggcgacgcgc
     1561 gttggcccaa ccgtcggcac cgccgcggcg caaccggcgc cgcccgcgcc cgcgccgcaa
     1621 gcgccgggca acgggctcct cccggcgatg gtcctgccgc tgcaaccgcc ggattggatt
     1681 gacagcccaa tcgacgccga ggcgacgtcg aagagcgtcg gcacgcgatt gaagaccgcc
     1741 caggttgccg aagccgcagc aacggagcag ttgcggcgga agtttttgga actgcacctc
     1801 acgccgcaga tgacgttggg cgatgcggta aagacggacg ggcgattggg cgatgccgtc
     1861 gatcgcgcga tgctgcacgc gcggatcgtg aaggtcgatt atcgaccgga tggttcggtg
     1921 aatgcgcgcg tgaggctcga cacgcaaagc gcgtgggacg agctgcggtc gaatccgtaa
     1981 tgtggcacgg gcgatcagcc cgtgattcgt tggtatgcca cggatgtcgg cggcacgccg
     2041 tggatgaagc aatcgcgcaa gcgacgagat ttcgccaact gtcgctgacg cgattgctgc
     2101 gtcgatgggt acatcgaccg tgcgtgacta cacgcaccac gggcgagacg cccgtgctac
     2161 ttaatatcca ctaaccaacc cgcgcctccg ctgtccatga cattcgagac gccccatctt
     2221 cccgctctat tgggaaaata gcagttgtcg gaatagttgg gacgcgtcaa cgtttggctg
     2281 gcgactgcgg tactagagat cgacccgcga atggtcagat cggcccgcca tgtgagcggg
     2341 ccgcggaatc gatcgacagg cacgcggaca atagatagag gagacgtcca atgtttcgtt
     2401 ccatgaagtg gattgccccg gttgcggggt tggcgttgtt tctgggtttc aaccaggtaa
     2461 ccaaggccgc cgatgaagcg ccggccgccg gtaaggcgac ggtcaccgtg acggtgcagg
     2521 acaaggacgg caagggcgtc gaaggggtcc gcgttcgcct ggtgtcggcc accccgccga
     2581 agacagaagg tggcgctgaa gccaaggccg ccggtgacgg tggcaaaaag ggcggcggga
     2641 agatggcctc ggtcgcgcaa ggcaccaccg acgccagcgg caaggccgtg ttggcgaacg
     2701 tgcccgatgg cgaatacacc gtgatggcaa gcctcaaggg cggcggcgcc ggtcgcgaaa
     2761 ccgtcaaggt cgaaggcggc aaagacgtgg cggtgaccgt cacgttgaag gacaagaagc
     2821 ccaaggccga agcccccccg gcggaagcga agtaagcttc atctgcaaca atctgccttt
     2881 gcgaattcag caggggatgc aacgaagagc cgctcgaact ttcgagcggc tcttcttgtt
     2941 cggtagcgcg ccctcgacgc cggtgtctga ggtac
//
LOCUS       DATIMH010001122         8674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2005047, whole genome shotgun sequence.
ACCESSION   DATIMH010001122 DATIMH010000000
VERSION     DATIMH010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005047"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VLI90_19345"
     CDS             complement(<1..470)
                     /locus_tag="VLI90_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16427.1"
                     /translation="MRIGINAFPLRAEGGGARYVFAGLLASLLKLDQEHHYLIFCHLE
                     SLCLVYQILRDHGGETLGGTGPDPRIKVVHVQDETQIFGHRFEFDLLFGPLNNLQPRL
                     YDRPTVAILHDIQEQYYPEYFSKSDLVARREIYPEICRSATTLIAISEFCKQSF"
     gene            685..1476
                     /locus_tag="VLI90_19350"
     CDS             685..1476
                     /locus_tag="VLI90_19350"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV16428.1"
                     /translation="MVLWKYRRFILANAFSDLRHRFSGSVGGYLWNVFIPLAQLLIFA
                     LIFSVLQGAHMTQGPQNRFSFILFLCSGLLAWNAFAETLVRACAALVGNAGYLKKLPL
                     PEQIFVAQEATSGFFTAAISIALFILFSILIAGYGPFWTWLQALPLLLLFICFAYGLG
                     LMLSCFNVFFRDVQPFMNVVVLLWFWLTPVVYFEDTFAKSPLVLKLLHANPAYYFIDA
                     FHQSLWLGQWVSRIDWLVCIGLTLVANLLAVTVVRRFRAELRDVL"
     gene            1503..2915
                     /locus_tag="VLI90_19355"
     CDS             1503..2915
                     /locus_tag="VLI90_19355"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV16429.1"
                     /translation="MTESSSLPAQASAAAEVIVQARGLTKRFKHYPTPAARLSEWLSF
                     GALHRHSLFTALDDLSFTIRRGEFFGIIGPNGSGKSTLLKVLTGVLMPTAGQFRVSGR
                     ITSLLELGTGFNPELSGRANLINSGRLIGLEPAVTRRKLGEIVDFAELADSIDVPIKY
                     YSTGMVVRLAFALFAHVDPDVFIVDEALSVGDIAFSRKCFERLDRMRASGCTLLFASH
                     DIAAIRKYCDQVLFLNHGKPAFLGNALDATDLYLEVMSPGAQAKGLRPDTVAMGYATP
                     PVQVPRDTLPPELAEAFDEASFAQIAELTSARIGTNDARIVALKITDADGVRTSSFTI
                     GDTMKLHLLAHVLRDVDYATVSFQLVNRMGIGVWGTNHALRSGQTTPLRAGQWLYTVF
                     EVKLDIGHDHYSVDVGWGDAAGQGHVFDRITGAATIIVNPAVHSDFIGLVRLACDSII
                     QQFDGAPPPPPDTPAAQEHP"
     gene            2912..3766
                     /locus_tag="VLI90_19360"
     CDS             2912..3766
                     /locus_tag="VLI90_19360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HSV16430.1"
                     /translation="MNATESDVSLSTTDPELVTHELTYGDQRLTIANFASDQLINTSL
                     AADGSWEPWQLKLMSRAVRPDFTCVDIGANIGINALFMSRCCPQGRVFAFEPFDRIYA
                     VLRRNVELNHTSNLTAINKGISSKTQSLNMVTDMRSVGGAHIAEPGATGGDVVGGTFH
                     FARLDDELRAQGVDKVDFIKIDVEGHELHVLDSAASFLSNADLQLAIEYNPPLLRITA
                     PDPSVPFVDRRLFDRLRLSFQRMFFMNRDGTLVELADYHALRRCLLGGYFVDDMYCTN
                     HVPASWST"
     gene            3730..4194
                     /locus_tag="VLI90_19365"
     CDS             3730..4194
                     /locus_tag="VLI90_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16431.1"
                     /translation="MHEPRAGQLVDMINPTFHAPAGLPVSMTVTPSFTATWYNRGEDG
                     WTSADSHSPATASLAISGGAASALRLRFDPIYKKHLEDGAIASWPVHVLVGDAAVTLD
                     LLDAPREVVIYVGERGPSVTIEADRSAPASSYLGNPADTRRVGFKCDVHLSS"
     gene            complement(4650..5849)
                     /locus_tag="VLI90_19370"
     CDS             complement(4650..5849)
                     /locus_tag="VLI90_19370"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HSV16432.1"
                     /translation="MNSVVSDKPYVPVLPYHGTYWPRMLNRYIPRYLHRRFGVAKVDI
                     VGAEKLRASIEAGHGILVTPNHCRDEDPFILSTLARKVGHPFFVVASAHLFMHNKLQA
                     FVLRRAGAFSIYREGMDKQAVQTSIEILETAERPLVIFPEGHISRTNDRLTPMLEGTA
                     LIARQAAKKRAKENKKVVVHPVSLRYSFPFDVDAVVARMLEEIEQRLTWRPSRGLPLH
                     ARFTKVGAGLLALKELEHLGHTQDGDIPERITKLIDALLTPMEAEWVNRNHDGHPVAR
                     VKRLRAAILPDIIKGELDETEKARRWRLLENADLAQQLYHYPPHYVGDGSSEARMIET
                     VERFEEDLTGKVSVHGPVNATVTVGDAIEVSTGREARGEADPLMSAIESQLRQMLGTK
                     TDAAPSS"
     gene            complement(6071..6784)
                     /locus_tag="VLI90_19375"
     CDS             complement(6071..6784)
                     /locus_tag="VLI90_19375"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HSV16433.1"
                     /translation="MSDGDTSTFQRRRLIVNADDFGLSPGVNAGIIEAHERGIVTATS
                     LMVRWPAAESAADYARSHPRLDVGLHLDLGEWICRDGAWVPLYDVISLTDATTVTAEV
                     DRQLSAFRRLLGRDPSHLDSHQHVHRDEPVRSIMTAVARRLRVPLRHVTPGYQYFGGF
                     YGQNDEGQSYPELVSADAMVALLSDLPAGTTELGCHPARGRDIEAMYVDEREREVETL
                     CDPRVRAAIAANHIDLTGF"
     gene            complement(6781..7962)
                     /locus_tag="VLI90_19380"
     CDS             complement(6781..7962)
                     /locus_tag="VLI90_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16434.1"
                     /translation="MIASGNKLRLVILGMMGRCPFGGQTWLYLNWLRALASQGHEVWY
                     VEDDTVWPYDPVKELVTDDCTYAARHIAASLKRIGLEGRWAMRLADRPDACHGISSAK
                     LNELYRDCDALLNIVGATDLRDEQMAAPLRVYVETDPVTAELRLASGDEHTRAAFANH
                     QVIVTYGENYGAADCGVPLNGISYKKTRQPIDTELWPMAFDPNAKYFTTIGNYRQEGS
                     DVEYNGQSYRWSKHHEWEKFINLPRRVNQPFELAMKIDDPNDTLFLESNGWRVISPVR
                     MSLDIFGAYPEYFRRSRAEFTVAKDQNIRLRSGWFSERDVCYLASGKPVVTQDTGFTS
                     VLPTGAGLFAFNTMEQAVAAVDEINRDYARHCSAAREMAEEYFAAPKVGAKLLKDIGL
                     V"
     gene            complement(7959..>8674)
                     /locus_tag="VLI90_19385"
     CDS             complement(7959..>8674)
                     /locus_tag="VLI90_19385"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV16435.1"
                     /translation="LHLFSQLYLVQNPREQMLTKPDPLWPTVYIPSWLQPGLIPRDPQ
                     RGQAFENIAYYGWPQNLVDPLNESKWRNELADMGLHWRIVSRSQWHDYTDTDAVVAIR
                     SFSDKSFGYKPAAKLFNAWHAGVPCVAGPESSFRAERCNELDYLEAKSFDEAVAAMRR
                     LRDDPALRRAMVENGNVRARETSCATMSHRWATILRDVVAPAWQRWRALSPAEQDQLL
                     DRRAATVQTVAAAPQGQQA"
BASE COUNT         1557 a         2723 c         2644 g         1750 t
ORIGIN      
        1 atgaacgatt gcttgcagaa ttcgctgatg gcgatcagcg tcgtggctga gcggcaaatt
       61 tccggataaa tctcacgccg ggcgaccagg tcggacttgc tgaaatactc agggtaatac
      121 tgttcctgga tgtcgtgcaa aatcgcgacg gtggggcgat cgtacaggcg cggttgcaga
      181 ttattcagcg ggccgaacag caggtcgaat tcgaagcggt gtccgaagat ctgcgtttca
      241 tcttgaacgt gaacgacctt gatgcgcgga tcggggcccg tgccgccgag cgtttccccg
      301 ccgtggtcgc ggaggatctg gtagacgagg cacagcgatt ccaggtggca gaaaatgaga
      361 tagtggtgtt cctggtcgag cttaagcagc gacgcgagca ggcccgcaaa tacataacgt
      421 gcgccgccgc cttcggcccg cagggggaac gcgttgatgc caattcgcat ataaaaaatg
      481 gcgcgcctta accggtcgtg ctattacgtt tacccgtgat gctgccccgg tccatcggca
      541 gcgctcttct cccccggtca gacatttgta tcatctacac ccctcgccgt cgatgcgacg
      601 gtggtgtgga atctgtaacc ggcgcgcctg acgacttatc accgcgtgcc cgcaaccgtc
      661 gcggccatcc gcactgacgc tgaaatggtt ctctggaaat accgacgatt catcctggcc
      721 aacgccttca gtgatctgcg tcaccgattc agcgggtccg tgggcggtta cctgtggaac
      781 gtgttcatcc cgctggcgca gctgctgatt ttcgcgctga tcttcagcgt gctgcagggc
      841 gctcacatga cgcaagggcc gcagaatcgc ttctcgttca tcctcttcct ttgctccgga
      901 ctgttggcgt ggaacgcatt tgccgaaaca ctcgtgcgag cgtgcgccgc attagtcggc
      961 aacgctggtt atctgaaaaa attgccgctg ccggagcaga tctttgtcgc gcaagaggcg
     1021 accagcggat ttttcaccgc cgcaatttcg atcgccctgt tcatcctctt ctcaatcctg
     1081 atcgcgggtt atgggccttt ctggacctgg cttcaggcgc tgccgctgtt gctgctgttc
     1141 atctgctttg cgtatggact gggcctgatg ctgtcctgct ttaatgtctt ctttcgcgac
     1201 gtgcagccgt tcatgaacgt cgtcgtgcta ctgtggtttt ggctcacgcc ggtcgtttat
     1261 ttcgaagata cattcgcaaa gagcccgctg gtgttgaagc tgttacacgc aaatcccgcg
     1321 tattatttca tcgatgcgtt tcaccagagc ctctggctgg gacaatgggt ctcgcgcatc
     1381 gattggctcg tctgtatagg tctgacgctg gtggcgaacc tgctcgcggt aacggtcgtc
     1441 cgccgcttcc gcgcggaact gcgtgatgtg ctatgacgtc tgccgtgctc gatccaaccc
     1501 cgatgaccga atcatcctcg ctgcccgcgc aagcatccgc ggcggccgag gtgatcgtgc
     1561 aggcgcgcgg actgaccaag cgcttcaagc attatcccac gccggcggcg cgattgagcg
     1621 aatggttgtc gtttggcgcg ctgcatcgcc acagtttgtt caccgcgctg gacgatcttt
     1681 cgttcaccat ccgccggggg gaattcttcg gcatcatcgg gccgaacggc tcgggcaaat
     1741 ccacgctgtt gaaggtcctc accggcgtgc tgatgccgac ggcggggcaa ttccgcgtca
     1801 gcgggcggat cacgagtctg ctcgagttgg gcaccggttt caatccagaa ctatccggtc
     1861 gcgcgaacct catcaacagc ggccggctga tcggcttaga gcccgcggtg acgcggcgga
     1921 agctgggcga gatcgtcgac tttgcggagc tcgccgattc gatcgacgtg ccgatcaagt
     1981 attactcgac cggcatggtt gtccgcctgg cgttcgcgct gttcgcccat gtcgatccgg
     2041 atgtgttcat cgtcgatgaa gcattgagtg tcggcgacat cgcgttctcg cgaaaatgtt
     2101 tcgagcgcct cgatcgcatg cgcgccagcg gttgtacgct gctattcgcc agtcacgaca
     2161 tcgcggccat ccggaaatac tgtgaccaag tgctgttcct gaaccacggc aaaccggcgt
     2221 tcctcggcaa cgcgctggat gcgacggacc tttacctgga agtgatgtcg cccggcgcgc
     2281 aagccaaggg cctccgaccg gacacggtgg cgatgggtta cgcgacgccg ccggtgcagg
     2341 tgccgcgcga tacgctgccg ccggagttgg cggaggcgtt tgacgaagca tcgttcgcgc
     2401 agatcgccga gctgacctcg gcccgcatcg gcaccaacga tgcccgcatc gtcgcgctca
     2461 agatcaccga cgctgacggc gttcgcacca gcagcttcac gatcggcgac acgatgaagc
     2521 tgcatctgct cgcgcacgtg ttacgcgatg tcgattacgc caccgtcagt tttcaattgg
     2581 tcaaccgcat gggcatcggc gtgtggggca ccaaccacgc gctgcgcagc gggcagacca
     2641 cgcccttgcg cgccgggcag tggttgtaca cggtgttcga ggttaagctc gacatcggtc
     2701 acgaccatta cagcgttgac gtcggctggg gcgacgcggc ggggcagggg cacgtgttcg
     2761 accgcatcac cggcgctgct accatcatcg tcaaccccgc ggtgcacagc gatttcatcg
     2821 gcctcgtgcg attggcgtgc gactcgatca tccagcagtt cgacggcgcg ccgccgccgc
     2881 cgcccgacac acccgccgcc caggagcatc catgaacgcc accgagtccg acgtgtcgct
     2941 ttcgaccacc gatcccgaat tggtcacgca cgagctgacc tacggcgatc aacgcttgac
     3001 gatcgctaac ttcgcgagcg accaattaat caacacgtcg ctggccgcag atggttcgtg
     3061 ggaaccctgg cagctgaagc tgatgagccg tgcggtgcgg ccggatttca cctgtgtgga
     3121 tatcggcgcg aacatcggca tcaacgcgct gttcatgtcg cgctgctgcc cgcaggggcg
     3181 cgtcttcgcg tttgaaccgt tcgaccggat ttacgcggtc cttcgccgca acgtggagct
     3241 gaatcacacg tcgaacctga cggccatcaa caagggcatc tcgtcaaaga cacagtcgct
     3301 gaacatggtg accgacatgc gcagcgtcgg cggggcgcac atcgccgagc ccggcgccac
     3361 cggcggcgac gtcgtgggcg gcaccttcca cttcgcgcga ttggatgacg agctgcgcgc
     3421 gcagggcgtt gataaggttg acttcatcaa gatcgacgtc gaggggcatg aactgcatgt
     3481 gctggacagc gctgcctcgt ttctgagcaa tgccgatctg cagctggcca ttgaatacaa
     3541 cccgccgctg ctgcgcatca cggcgcctga cccgagcgtg ccgttcgtgg accgccggct
     3601 gttcgatcgc ctgcggcttt cgttccagcg catgttcttc atgaaccgcg acggcacgct
     3661 agtcgagctg gccgattatc acgcgcttcg ccgctgcctg cttggcgggt atttcgtcga
     3721 tgatatgtat tgcacgaacc acgtgccggc cagctggtcg acatgatcaa ccccacgttc
     3781 cacgccccgg cggggctgcc ggtcagcatg accgtgactc cgagcttcac cgcgacatgg
     3841 tacaaccgag gcgaagatgg ctggacgtcg gccgactcgc acagtcccgc caccgcatca
     3901 ctggccatca gcggcggagc cgcgtccgcc ctgcggctgc gattcgatcc gatctataag
     3961 aagcatctgg aagatggcgc gatcgccagt tggcccgttc acgtcctcgt tggcgacgcc
     4021 gcagtgacgc tggacctatt ggatgccccg cgcgaagtcg tgatctacgt tggcgagcgc
     4081 ggcccgtcgg tgacgatcga agccgatcgc agtgcgcccg ccagcagcta cctgggcaac
     4141 cccgccgaca cccgccgggt ggggttcaag tgtgacgtgc atttgtcatc ctgaagcgca
     4201 ggcgtactgg ccggagtcga aggatctaaa gtaatcgcta tcaagttttt ccgaatgcgc
     4261 caggaacgat gaatttcgca acgaccgtcg ttgaagaaac gcattccacg ccgggttcgg
     4321 agcgtactcg cgaagaagcg agattcttcc aagttcgcaa aaagcatccg ggtcgtcgga
     4381 gtacctccga acccggcgtt gagcggggcg atcctttcca gcgtctgcat gactgatcgg
     4441 atttgattgg tcgcacttac gtagctcgcg cgtctgtgaa ccgtggcacg cgcaaaatcg
     4501 cgcggcagtc gcttgcggtt tcgcagacca atgaccgcgg cttgtccgtc tgcgaaactg
     4561 cgcgactgcc gtgcgtttgt ggcaactgaa cgttcgtcca cttctccgct agatccttcg
     4621 actgcgcacg agtacgtgct tctttcgctt caggatgacg gtgccgcatc tgtcttagtc
     4681 ccgagcatct gtcgcagttg cgattcaatc gcgctcatca gcgggtccgc ttccccgcgg
     4741 gcttctcggc cggtgctgac ttcaatcgca tcgcccaccg tgacagtcgc gttcaccgga
     4801 ccatgcacgc tgactttgcc ggtcaggtct tcttcgaacc gctcgaccgt ttcgatcatg
     4861 cgggcctcgg agctgccgtc gccgacgtag tgcggcggat agtggtagag ctgctgcgcc
     4921 agatcggcgt tctccagcaa ccgccagcgc cgagctttct ccgtctcgtc cagctcgccc
     4981 ttgatgatgt ccggcaggat ggccgcgcgc agccgcttca cgcgcgcgac gggatggccg
     5041 tcgtgattcc ggttgaccca ctccgcttcc atcggcgtca gcagcgcgtc gatcagcttc
     5101 gtgatgcgct cggggatgtc gccatcctgc gtgtggccga gatgctccag ctccttcagc
     5161 gccagcaacc cggcgccgac tttggtgaac cgcgcgtgca gcggcagtcc gcggctgggt
     5221 cgccaggtca ggcgctgctc gatctcctcg agcatccgcg ccaccaccgc gtccacgtcg
     5281 aacgggaacg aatatcgcag cgagaccgga tgcacgacga ccttcttgtt ctccttcgcg
     5341 cgcttcttgg ccgcctgccg cgcgatcagc gcggtccctt cgagcatcgg cgtcagccga
     5401 tcgttcgtgc gcgagatatg cccctcgggg aagatgacca gtggccgctc ggcggtctcc
     5461 aaaatctcga tcgacgtctg caccgcctgc ttgtccatgc cttcgcggta aatcgaaaac
     5521 gccccagccc ggcgaagcac gaacgcctgc agcttgttgt gcatgaacag gtgagcgctg
     5581 gcgacgacga aaaatggatg cccgactttc cgcgctagcg tgctgaggat aaacgggtct
     5641 tcatcccggc aatggttcgg cgtgaccagg atgccatgcc cggcctcgat tgacgcgcgc
     5701 agtttctccg cgccgacgat atcgactttg gccacgccaa accggcgatg caggtagcgc
     5761 gggatgtatc ggttcaacat ccgcggccaa taggtgccgt ggtaggggag aaccggaacg
     5821 tagggcttgt ccgatacaac cgagttcatg cgtggcggca tcttcaatga actgaggccg
     5881 cttttccagt tagctcattg atcgcaacac gagttcaaga ggattaacca cagatgaaca
     5941 cagatgatgg aaaagtagca ccgtcgtctc gcccgtgctg agcggcgcca aacacgggtg
     6001 agacgcccat gctacatttc tgatctgtgt tcatccgtgt gcatctgtgg ttaatcgtct
     6061 tccattccat tcagaatccc gtgagatcga tgtggttcgc agcaatggcc gcgcggacgc
     6121 gcgggtcgca cagcgtttca acctcgcgtt cgcgttcgtc gacgtacatc gcttcaatgt
     6181 cgcgcccacg ggcggggtgg cagcccagtt ccgtggtgcc agcgggaaga tcgctcagca
     6241 gcgcaaccat cgcatcagcg ctgaccagct cggggtacga ttggccctca tcgttctgcc
     6301 cgtaaaatcc gccgaaatat tggtagcccg gcgtcacgtg acgcagcggc acgcgaaggc
     6361 ggcgcgcgac ggcggtcatg atggatcgca ccggctcatc gcgatgcacg tgctgatgcg
     6421 aatcgagatg cgacggatcg cggccgagca atcggcggaa cgccgacaac tgccgatcga
     6481 cttccgcggt gaccgtcgtt gcatccgtga gggaaatgac gtcgtacagc ggtacccagg
     6541 cgccatcgcg gcagatccac tcccccaggt ccaggtgcag accgacgtcg agccgcgggt
     6601 gcgatcgcgc gtaatccgcc gcggattcgg cggcgggcca gcggaccatc aggctggtcg
     6661 cggtgacaat accgcgctcg tgcgcttcga tgatgcccgc gttgacaccg gggctcaagc
     6721 cgaaatcgtc ggcgttgacg atcaaccgcc ggcgttgaaa tgtggaggtg tcgccgtcgc
     6781 tcataccagg ccgatgtcct tcagcagctt cgctccgacc ttcggcgctg caaaatattc
     6841 ctcggccatc tcgcgcgccg ccgagcagtg ccgcgcgtaa tcgcggttga tttcgtcgac
     6901 cgccgccacc gcctgctcca tcgtgttgaa cgcaaacagc cctgccccgg tcggcagcac
     6961 gctggtgaac ccggtgtcct gcgtgacgac cggcttcccg ctggcgagat aacacacatc
     7021 gcgctcgctg aaccaaccgc tgcgcaggcg gatgttctgg tccttcgcga cggtgaactc
     7081 cgcgcgcgag cggcgaaagt attcgggata ggcgccgaaa atgtcgagcg acatccgcac
     7141 gggcgaaatg acgcgccagc cgttggattc gaggaagagc gtgtcattcg ggtcgtcgat
     7201 cttcatcgcc aactcgaacg gctggttcac ccgccgtggg aggttgatga acttctccca
     7261 ttcgtgatgc ttgctccagc ggtagctctg accgttgtat tcgacgtcgc tgccttcctg
     7321 gcgatagttg ccgatcgttg tgaagtattt cgcgttcgga tcaaaggcca tcggccaaag
     7381 ttcggtgtcg atcggctggc gcgtcttctt gtacgagatg ccgttcaggg gcacgccgca
     7441 atccgccgcg ccgtagttct cgccgtaagt gacgatcacc tgatgattgg caaacgcggc
     7501 gcgcgtgtgt tcgtcgccgg acgcgagccg caactccgcc gtcaccggat cggtctcaac
     7561 gtacacgcgc aacggcgccg ccatttgctc gtcgcgcaga tcggtcgcgc cgacgatgtt
     7621 cagcagcgca tcacaatcac gatacaactc gttcagcttc gccgaggaga tgccatggca
     7681 agcatccggc cgatcggcca gccgcatcgc ccagcgacct tccagaccga tgcgcttcag
     7741 actggcagcg atgtgccgag ccgcataggt acagtcgtcg gtgaccagct ccttgaccgg
     7801 atcgtacggc cagacggtgt cgtcctcgac ataccagacc tcatgcccct ggctcgccag
     7861 cgcgcgcagc caattgaggt agagccacgt ctgcccgccg aagggacagc ggcccatcat
     7921 gccgaggatg accaggcgaa gtttgttacc agacgcgatc atgcctgctg gccctgtggt
     7981 gccgcggcga ccgtttgaac agtcgccgcg cgacggtcca gcagctgatc ctgttcggct
     8041 ggcgaaagcg cacgccatcg ctgccacgcg ggcgccacga catcgcgcag aatggtggcc
     8101 cagcgatgtg acatcgtcgc gcacgatgtt tcgcgagcgc gaacgttgcc attttccacc
     8161 atggcccgcc gcaacgccgg gtcgtcgcgc agtcgccgca tggcggcaac ggcctcgtcg
     8221 aacgacttcg cttcgaggta atcgagctcg ttgcaacgct cggctcggaa tgacgactct
     8281 ggcccggcga cgcatgggac gccggcgtgc caggcattga acaatttcgc cgccggtttg
     8341 taaccgaacg atttgtcgga gaaactgcga atggcaacga ccgcgtcggt gtcggtgtaa
     8401 tcgtgccatt gcgaccgcga cacgatgcgc cagtgcagcc ccatgtccgc cagttcattt
     8461 cgccactttg attcgttcag cgggtcaacc aggttttgcg gccagccata ataggcgatg
     8521 ttctcgaacg cctggccgcg ctgcgggtcg cgcgggatca ggcctggttg cagccacgac
     8581 gggatgtaga cggttggcca cagcgggtcc ggcttggtca gcatttgctc gcgaggattc
     8641 tggaccaggt acaactgcga gaacaagtgt aagc
//
LOCUS       DATIMH010001123         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2286942, whole genome shotgun sequence.
ACCESSION   DATIMH010001123 DATIMH010000000
VERSION     DATIMH010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2286942"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="VLI90_19390"
     CDS             complement(<1..1717)
                     /locus_tag="VLI90_19390"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSV16436.1"
                     /translation="MHIASCLVIITLAVFAAGAHAQLGETAFDDGWRFAHADPTGAEA
                     PAFDDTRWESVDLPHDWAIAGPIAAENPTGSPGGFFPAGVGWYRKSFVAPREWSGKRV
                     AIEFDGAYMRTDAWLNGQKLGTHATGFTPFTFDLTSNLNVGATNTIAVRVDNSRQMNC
                     RWYSGSGIYRHVRLDVRELVCIAQYGICIRTDAVSDGSAKLSMSITAENAGGQANPHL
                     EYATQVFELGLDGKPSGNAVTSFAPATADFKDHTSTVDTSATLLNPKLWSPVTPNRYI
                     AVTTASSDGHVIDTRETTFGVRTTAASADRGFLLNGESIKIYGACVHDDNGALGTAAF
                     DRAEARRAEMLKAAGFNAVRCAHNPPAPAFLDACDRLGLLVIDESFDSWAYGKLPQDY
                     SVDFAANWQADLDAMILRDRNHPSIVMWSLGNEIHDFGSAQGLRNGMAIVARARALDP
                     TRLLTTAVFWYRTIGGPLHWKWEDADELMSKFDVVGYNYQINRYEADHQRVPSRVMLG
                     TESFPNDLFVSWDSSTKLPYVLGDFVWTGIDYVGESGIGRSYAPGEKVLFHAIAEQYP
                     YHGAYC"
     gene            complement(1810..>2609)
                     /locus_tag="VLI90_19395"
     CDS             complement(1810..>2609)
                     /locus_tag="VLI90_19395"
                     /inference="COORDINATES: protein motif:HMM:NF036499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16437.1"
                     /translation="ENHPARRPHRRRWGGDRRHRYRRRRIQRREGGDDDATEITRWRD
                     YLTEQVKNYNQRVAGGKWNHVMPGLVTGKVISAWSSQVRWPWGEKGPATTTTSAPASE
                     PVIEATPVRPWRGAAACDRQTNTAAGARWVAVAGLGPSGRAMSLQPATLETSFQDGHG
                     PCLQFDFQSDRAGDAEALLDFLPTFRLVPGMQLRVAVSVDDGPPRMLEIPGSSGKEDE
                     RGPVRTLAVQANYVRARVPLSALSAGKHTFKIYAVDPGAVLDRVSLP"
BASE COUNT          424 a          833 c          883 g          469 t
ORIGIN      
        1 cgcagtaggc gccgtgatat gggtactgct cggcgatggc gtggaataag accttttcac
       61 ccggcgcgta cgatcggccg atgccggatt caccgacgta atcgatgccg gtccagacga
      121 aatcgccgag cacgtacggc aacttcgtcg aggagtccca gctgacgaac aggtcattcg
      181 gaaacgattc ggtgccgagc atcacgcggc ttggcacgcg ctggtggtcc gcttcgtagc
      241 ggttgatctg atagttgtag ccaaccacgt caaacttcga catcagctca tcggcgtcct
      301 cccacttcca gtgcagcggt ccaccaatgg tgcgatacca gaagaccgcg gtggtgagca
      361 ggcgcgtggg atccagcgcg cgggcgcgcg cgacgatcgc catgccgttg cgcaagccct
      421 gcgcgctgcc gaagtcgtgg atctcgttgc cgaggctcca catcacgatc gagggatggt
      481 tgcggtcgcg caggatcatc gcgtccaggt ccgcctgcca gttggcggcg aaatcgacgc
      541 tgtaatcttg cggcagcttt ccgtaggccc agctgtcgaa ggattcgtcg atgacgagca
      601 ggccgagacg atcgcacgca tcgagaaacg ccggcgccgg tgggttgtgg gcgcagcgca
      661 ccgcgttgaa ccccgcggcc ttgagcatct cggcccggcg agcttcggcg cgatcgaacg
      721 ccgctgtgcc gagcgcgccg ttgtcatcgt ggacgcacgc gccatatatc ttgatcgact
      781 cgccgttgag aaggaaacct ctgtcggccg acgcggcggt ggttcgcacg ccgaaggtcg
      841 tttctcgcgt atcgatcacg tggccgtcgg atgaggcggt ggtgacggcg atgtaccggt
      901 tcggcgtgac cggactccac agcttcggat tgagcagcgt ggcgctggta tcaaccgttg
      961 aggtgtggtc tttgaagtca gcggtggccg gcgcgaagct cgtgacggca ttgcctgacg
     1021 gtttaccatc gaggccgagc tcgaacactt gcgtcgcgta ttccagatgc ggattcgctt
     1081 gaccaccggc gttctccgcg gtgatcgaca tgctgagttt tgccgagccg tcggacaccg
     1141 cgtcggtgcg aatgcagatg ccgtactggg cgatgcaaac cagctcgcgc acgtcgagcc
     1201 gcacgtgccg atagatgccg gagccgctgt accaccggca gttcatctgc cgcgagttgt
     1261 cgacgcgcac cgcaatggtg ttggtcgcgc cgacgttcaa gttggatgtc agatcgaacg
     1321 tgaacggcgt gaaaccggtc gcgtgcgtgc cgagcttctg gccgttcagc cacgcatcgg
     1381 tgcgcatgta cgcgccgtcg aactcgatcg ccacgcgttt gcccgaccac tcgcgcggcg
     1441 caacgaacga cttgcgatac cacccgacgc ctgcggggaa gaacccgccc gggctgccgg
     1501 tgggattctc cgccgcgatc ggcccggcga tggcccagtc gtgcggcagg tccacggatt
     1561 cccaacgcgt atcgtcgaag gcgggcgcct cggcgccggt cggatcagcg tgtgcgaacc
     1621 gccagccatc gtcgaacgcg gtttcgccga gctgcgcgtg cgcgccggca gcgaagacgg
     1681 ccagagtgat gatcacaagg catgatgcga tgtgcaaagc aaatcctccc gcggatgaag
     1741 gtgccgcgat gcgatgtgat cttctgcttt tgcgttcgcg cggcaagagc ggctcgtttt
     1801 tcgcgcgcat cacggcagcg agacgcgatc gaggaccgca cccggatcga cggcgtagat
     1861 tttgaacgtg tgcttgccag cgctcaacgc cgacagcggg acgcgggcgc ggacgtagtt
     1921 cgcctgcacg gccagcgtgc ggacgggacc gcgttcatct tccttgccgc tggagccggg
     1981 gatttccaac atccgcggtg ggccgtcgtc cacgctcacg gcgacgcgca gttgcatgcc
     2041 cggcacgagg cggaaggtcg gcaggaaatc gagcagcgct tcggcatcgc cagcgcggtc
     2101 ggactggaag tcgaactgca ggcacgggcc gtggccatcc tggaacgacg tctcgagcgt
     2161 tgccggttgc agcgacatcg cgcggcccga cggtcccaat cccgcgaccg caacccagcg
     2221 cgcgccggcc gcggtgttgg tctggcgatc gcacgcggcg gcgccgcgcc acgggcgcac
     2281 cggcgttgcc tcgatcaccg gttcagatgc cggcgccgat gtggtggtcg tcgccggtcc
     2341 cttttcgccc cagggccagc gcacctggct ggaccaggcg ctgatgacct tcccggtgac
     2401 caggcccggc atgacgtggt tccatttccc gccggccacg cgctggttgt aattcttcac
     2461 ctgctcggtg aggtaatcgc gccagcgcgt gatttccgtc gcgtcgtcgt cgccgccctc
     2521 gcgtcgctgg atgcggcgac gccggtatcg gtgacgacga tctccgcccc accgtcggcg
     2581 gtgcgggcgg cgtgcagggt gattttccc
//
LOCUS       DATIMH010001124         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1425793, whole genome shotgun sequence.
ACCESSION   DATIMH010001124 DATIMH010000000
VERSION     DATIMH010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425793"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="VLI90_19400"
     CDS             complement(<1..1758)
                     /locus_tag="VLI90_19400"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HSV16438.1"
                     /translation="MKQIAFAGVGLLLLTFLLPSSRAAAGADELFRDPSRSIDDRVTD
                     LISRLTLEEKATLLDHRGPDVERFHIASDKWNQCLHGVWWDRPTTMFPVSIALAATWD
                     LDLVHEETTAISDEARAIYNGWHDDPNFKGDHKGLIYRAPVVNVSRNPYWGRINECFG
                     EDPYLTGRMGVAYVKGLQGDDAKYLKLAATLKHYAVNNVEQNRKALNAIVPERMLYEF
                     WLPHFRDCIVEGQAQSIMASYNAINGTPNNINHLLLTEILKDQWGFKGFVVSDLGGVD
                     TMVKGHEKGKMGYEEAVAQSLNAGCDFSDKEFRMNIPGAVRAGLLTEARLNDALTRVL
                     RVRFRLGEFDPREAVPYSRISPDVIDNAAHRQLALKVARESIVLLKNADHLLPLDRAK
                     LKTIAVIGPHADVFTAGGYSGKVRNPVTPLAGIKNRAGAIEVLHAIGAQFGPPSLGEG
                     RPPRASTAPFDRDAEIAKAVDAAKRADVAILYVGTDLSIEGEGHDRPTLALPGEQEAL
                     VKAVLAVNPRTVVVEMNAGPITSPWMKQNVPAIIEAWWAGEEGGNAIADVLFGDVNPG
                     GKMPLTVYASADQVPPQDEY"
     gene            complement(1961..2452)
                     /locus_tag="VLI90_19405"
     CDS             complement(1961..2452)
                     /locus_tag="VLI90_19405"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSV16439.1"
                     /translation="MKKNGGALINVGSEVSDAVAPMLGMYSASKHAVKGFTDALRVEI
                     ERLDQAPVSITLIQPTAVDTPFDDHAKNYMEKAADLPTPMIDPEKGADAILSAAEKPT
                     RDVKVGMMAKVNTFTAKNLPGLHDRMSAKQVQNLKRDEPPMDREGALHAPSNSGQTHG
                     RHA"
     gene            2424..2684
                     /locus_tag="VLI90_19410"
     CDS             2424..2684
                     /locus_tag="VLI90_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16440.1"
                     /translation="MINAPPFFFKCGKAASEPIHHAPKVRVKQSPAVGLADFVEPAIN
                     RDARVVHPCVNASEALDSGIGDLLDGFTISHIRRDCDHLVAI"
BASE COUNT          462 a          930 c          777 g          548 t
ORIGIN      
        1 gtattcatcc tgcggcggga cctgatcggc cgacgcgtac accgtcagcg gcattttccc
       61 gccggggttg acgtcgccga acagcacgtc ggcaatcgcg ttgccgccct cttcaccggc
      121 ccaccacgct tcgatgatcg cgggcacgtt ctgcttcatc cacggcgagg tgatcggccc
      181 ggcgttcatc tcgacgacga ccgtgcgcgg gttcacggcc agcaccgctt tcaccagcgc
      241 ttcctgttca cccggcaacg caagcgtcgg gcgatcgtgc ccttcgccct cgatcgagag
      301 atcggttccg acgtacaaga tcgcgacgtc agcgcgcttc gccgcgtcaa cggcctttgc
      361 tatctccgca tctcgatcaa acggcgccgt cgatgctcgc ggcggccggc cttcccccag
      421 cgacggtggg ccgaactgcg cgccgatcgc gtggagaact tcgatcgcgc ccgcgcgatt
      481 cttgatgccc gctagcggcg tcaccgggtt ccgcaccttc ccgctgtatc cccccgcggt
      541 gaacacatcc gcgtgcgggc cgatcaccgc gatcgtcttc agcttcgcgc gatccagcgg
      601 cagcagatga tccgcgttct tcaacagcac gatcgattcg cgcgccacct tcagcgccag
      661 ctggcgatgg gccgcgttgt cgatcacatc gggagagatc ctgctatacg gcaccgcctc
      721 gcgcgggtcg aactcgccca accgaaaccg cacgcgcagc acgcgcgtca gggcatcgtt
      781 cagccgggcc tccgtcaaca accccgcccg caccgcgccg gggatgttca tgcgaaattc
      841 cttgtccgaa aaatcacacc ccgcgttcag cgactgcgcc accgcttcct cataacccat
      901 ttttcccttc tcgtggccct tcaccatcgt gtccacgccg cccaggtccg acacgacgaa
      961 acctttgaat ccccactggt ccttgaggat ctcggtcagc agcaggtggt tgatgttatt
     1021 cggcgtgccg ttgatcgcgt tgtacgaggc catgatcgac tgcgcctgcc cctcgacgat
     1081 gcagtcgcga aagtgcggca gccagaactc gtatagcatc cgctccggca cgatcgcgtt
     1141 gagcgccttg cgattctgct cgacgttgtt caccgcataa tgcttcagcg tcgccgccag
     1201 cttcaagtac ttcgcgtcgt cgccctgcaa tcctttcacg tacgcgacgc ccatccggcc
     1261 ggtcaggtat gggtcttcac cgaaacattc gttgatgcgc ccccagtacg gattgcgact
     1321 gacgttgacg accggcgcgc gatagatcag gcccttgtgg tcgcctttga aattcggatc
     1381 gtcatgccag ccgttgtaga tcgcgcgcgc ttcatcggag atcgcggtcg tctcctcatg
     1441 caccaggtcc aagtcccacg tggccgccag cgcgatggag acggggaaca tcgtcgtcgg
     1501 ccgatcccac cagacgccgt gcaggcattg gttccacttg tcggacgcga tgtgaaaccg
     1561 ctcgacgtcc ggcccgcggt ggtcaagcag cgtggctttt tcttcgagcg tcagtcgcga
     1621 gatgagatcg gtgacgcggt cgtcgatcga gcgcgatgga tcgcggaaca attcgtcagc
     1681 gccagcggcc gcgcgcgacg acggaagcaa gaacgtcagc aaaagcagac caacccccgc
     1741 gaacgcgatt tgtttcatat cgcgcaagaa tggctgctcc gagtcggagc gtcaagcgag
     1801 gtggaatgat ggaccagcca cgaaagccca cgcctatgcc ttggcctgtt catctcgtcg
     1861 cacggaatag ccgtgcgctt tgtttgctca aacgaagacg ccgccctgac cgtggcggcg
     1921 tcttcacgaa aacagcgctt ccccttgcta ttaagtcagc tcacgcatgc cgcccgtgcg
     1981 tttgcccgct gttgctcggc gcgtgcagcg cgccttctcg atccatcggc ggctcgtcgc
     2041 gcttcaggtt ctggacctgc ttggccgaca tccgatcgtg cagcccgggg aggttcttcg
     2101 ccgtgaaggt gttcaccttc gccatcatac cgaccttcac gtcgcgcgtc ggtttctcgg
     2161 ccgcactgag gatcgcgtcc gcgccctttt ccgggtcgat catcggcgtc ggcaggtcgg
     2221 ccgccttctc catgtagttc ttcgcgtgat catcgaacgg cgtatcaacg gccgtcggtt
     2281 ggatcaacgt gatcgacacc ggcgcctggt ccagccgttc gatctcgacg cgcagcgcat
     2341 cggtgaaccc tttgaccgcg tgcttggatg ccgagtacat gccgagcatg ggcgcgaccg
     2401 catcggacac ttcgctgccg acgttgatca acgccccgcc gttcttcttc aagtgcggca
     2461 aggcggcgag cgagcccatt caccacgccc caaaagttcg tgtcaaacag tcgccggctg
     2521 tcggcctcgc tgacttcgtt gagccggcca taaatcgcga cgcccgcgtc gttcacccat
     2581 gtgtcaatgc gtccgaagcg ctggacagcg gcatcggcga tttgctcgac ggctttacga
     2641 tcagccacat ccgccgtgac tgtgaccact tggtcgccat ctgagttcaa tcgtgaagca
     2701 atctgatcca gcgtatc
//
LOCUS       DATIMH010001125         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_848027, whole genome shotgun sequence.
ACCESSION   DATIMH010001125 DATIMH010000000
VERSION     DATIMH010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848027"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VLI90_19415"
     CDS             <1..1022
                     /locus_tag="VLI90_19415"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSV16441.1"
                     /translation="HKVSEPFLKAYSRDFQGKDEVTWRAITEGVATRADANSPYVREF
                     LKTFGYEYAAAAPLQAPVFDGYAGAIQVVRTADQGAFTDADLRKLSDAARRLDSLIAS
                     TRASRLGGGDNQPVWTSRPTTRQVVLDAHGKVRLFESGFEEVDERIRQQMVEQATQRV
                     AQLNGEAVTSNRVLLPDSAGDHWTYNAVVFKNYPALSDEPVVFFCLQPACPEWSTIRP
                     SDFQADAEVSRLVPALKFMQQEFHRSPTLVEISKTVHLSPFHFHRRFTELMGLTPKHF
                     LLECQIHAAKTQLLAGEKELAQIAADCGFAHQSHFTSRFKQATGLTPTRWRRMALDRN
                     KNSDN"
     gene            complement(1196..1672)
                     /gene="tadA"
                     /locus_tag="VLI90_19420"
     CDS             complement(1196..1672)
                     /gene="tadA"
                     /locus_tag="VLI90_19420"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HSV16442.1"
                     /translation="MNLATDEQWMREALTEARRAGDAGEVPIGCVIVHEPTNRIIGRG
                     YNRRETDEDPTAHAEIVAMRQAGQSLGHWRLLECALIVTLEPCPMCAGAIVNARIPRL
                     IYGCPDPKAGAVRTLFQLCEDPRLNHRVAVTAGVLSEECAALLTDFFRAQRLMGKR"
     gene            complement(1746..2138)
                     /locus_tag="VLI90_19425"
     CDS             complement(1746..2138)
                     /locus_tag="VLI90_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16443.1"
                     /translation="MTLPAQLLETTPTPRRPWRYDIPSLLIATERFCLQFGHRVGTVA
                     LFAMLAAWALLYRGQSYHWPLAIAFAGSNLGFCAALLARIIARGRSREALGLIIFNLL
                     ILALSLQLMLLSRLDVIRAAAERFFSPT"
     gene            complement(2153..2377)
                     /locus_tag="VLI90_19430"
     CDS             complement(2153..2377)
                     /locus_tag="VLI90_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HSV16444.1"
                     /translation="MRDRVQDVINLIRPAVQADGGDVELVDVLPDGTVHIRFHGACHG
                     CPSSTMTLQDGIERNLRERVPEVTRVLPVQ"
BASE COUNT          457 a          796 c          831 g          396 t
ORIGIN      
        1 cgcataaagt ctccgagccc ttcttgaagg cgtactcgcg cgacttccaa ggcaaggacg
       61 aggtcacttg gcgggcaatc accgaaggcg tggccacccg cgccgacgcc aactcgccct
      121 acgtgcgcga attcctcaag accttcggct acgaatacgc cgccgcggcc ccgctgcagg
      181 cgccggtgtt cgacggctat gccggcgcga ttcaggtcgt gcgcaccgcc gaccaaggcg
      241 cgttcaccga tgccgacctg cgcaagctga gtgatgccgc ccgccggctc gacagcctga
      301 tcgccagcac ccgcgccagc cggctgggtg gtggcgacaa ccagcccgtc tggaccagcc
      361 gtccgacgac ccggcaggtc gtgctggacg cccacgggaa agtccgcctg ttcgaaagcg
      421 gattcgagga agtcgatgag cgcatccgcc agcagatggt cgagcaggcg acgcagcgcg
      481 tcgcgcagct gaacggcgaa gccgtcacca gcaaccgcgt gctgctgccc gacagcgccg
      541 gcgatcactg gacgtacaac gccgtcgtct tcaaaaacta ccccgcgctc agcgacgagc
      601 cggtggtgtt cttctgcctt cagcccgcgt gccccgagtg gagcacgatc cgcccgtccg
      661 acttccaggc cgacgcggaa gtcagccggt tggtgccggc gctgaagttc atgcagcagg
      721 aatttcaccg cagcccgacg ctggtggaga tcagcaagac ggtgcatttg agcccgttcc
      781 acttccaccg ccggttcacg gaactgatgg gcctgacgcc caagcacttc ctgctggagt
      841 gccagatcca cgcggccaag acgcagctgt tggcgggtga aaaggaactc gcgcagatcg
      901 ccgccgactg cgggttcgcg caccagagcc acttcaccag ccgcttcaag caagcgaccg
      961 gtctgacgcc aacccgctgg cgccggatgg cactggaccg caacaagaac tccgacaact
     1021 gatcgatgag aagcaatcag attgcgaata acaaacaacc gccgccgggc catcccgccg
     1081 gcggttgttt ttttgaatag cgcgcgccgt caaagccgcg gccggaaggc ccggtctttt
     1141 tgcgttcgat acgaaaaaac accggggctt ccgcctccgg ctatgatggc ccgaatcacc
     1201 tcttgcccat cagcctttga gcgcgaaaga agtcggtgag cagcgccgcg cattcctccg
     1261 acaggacgcc ggcggttacg gcgactctgt ggttcaggcg tgggtcttcg caaagctgga
     1321 acagcgtgcg gaccgcgccg gctttggggt cggggcagcc gtagatcagg cgggggattc
     1381 tcgcgttgac gatcgcgccg gcgcacatcg ggcagggctc gagcgtgacg atcagcgcgc
     1441 actcgagcag tcgccagtgg ccgagcgact ggccggcttg gcgcatcgcg acgatctcgg
     1501 cgtgtgccgt cgggtcttca tcggtctcgc gacggttgta gccgcggccg atgatgcgat
     1561 tggtgggctc gtgaacgatc acgcagccga tcggcacctc accggcgtcg ccggcgcggc
     1621 gggcttcggt cagcgcttcg cgcatccact gctcgtcggt cgcgaggttc atgatcgatt
     1681 gcgcgattaa tgtagccgcg ccgcctgcgt cgcctcttcg cacgcgggcg acgcaggcgt
     1741 cgcggctacg tgggcgagaa aaatcgctcc gcggcggcgc ggatgacgtc gagccggcta
     1801 agcaacatca gttgcagcga cagggccagg atcagcaggt tgaagatgat caggccgagc
     1861 gcttcacgac ttcggcctcg tgcgatgatg cgcgcaagca gggcggcaca gaacccgagg
     1921 ttgctcccgg caaacgcgat ggcgagcggc cagtggtagc tctgcccgcg gtagagcagg
     1981 gcccaggcag ccagcatggc gaagagggcg accgttccca cgcgatggcc gaactgcagg
     2041 cagaagcgtt cggtggcgat cagcagcgac ggaatgtcgt aacgccacgg acggcgaggc
     2101 gtcggcgtcg tttccagaag ttgcgccggc agggtcatga tggatcgttg gattactgaa
     2161 cgggcaggac gcgggtgact tctggcacgc gctcgcgcag gttgcgttcg atgccgtcct
     2221 gcaacgtcat cgtgctgctg ggacagccat ggcaggcgcc gtgaaagcgg atgtgcaccg
     2281 tgccgtcggg caggacgtca accaattcga catcgccgcc gtccgcctgg acggcggggc
     2341 gaatcaggtt gatcacgtct tggacgcggt cgcgcagggg gctggcggtc gcaacattcg
     2401 cggaaggcat ttggagaatt gtagcgtcag aacggagcga atgccacttc tccacgcgta
     2461 gcggtagccc cgcaggggca
//
LOCUS       DATIMH010001126         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5471887, whole genome shotgun sequence.
ACCESSION   DATIMH010001126 DATIMH010000000
VERSION     DATIMH010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5471887"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1720
                     /gene="recG"
                     /locus_tag="VLI90_19435"
     CDS             <1..1720
                     /gene="recG"
                     /locus_tag="VLI90_19435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HSV16445.1"
                     /translation="VKVYRNLPQMTHPTWETIEQGTPRVEQSKFLAIYPASMRLASDV
                     IAKLVEDNINAALGEVHEWFDPQLLKLRGLLGRREAYRLIHCPANLREAVQARRRLIY
                     DELMLMQLGLGLSKRLRSGRLTAPVMRIDKMLDERIRKRFPFQLTTAQQNAIWEILHD
                     LQSGQPMNRLLQGDVGSGKTVVALYAMLVAVANRMQSALLAPTEVLAEQHYLTLSNLL
                     KDSNVTVELYTGRTKRQSRSGIAGLSKGTVHLAIGTQALIQEDIEFANLGLVVVDEQH
                     KLGVQQRAVLKSKGLAPHYLVMTATPIPRTLALSYFADFDVSSINELPPGRQPIKTKL
                     VRSREAAKAYDFVRQQVAKGRQAYIVLPRIDDDGLDDAKSVMKEFDRLSKGPLSGLRL
                     AVLHGQMSTEEKQATMLAFRDRQTDVLVATTVIEVGIDVPNATVMVIGDAERFGLSQL
                     HQLRGRVGRGTEISYCLLMSDAVGEPAESRLGAMTSTNDGFEIAELDLKLRGPGQFFG
                     TRQHGLPEFKLADITTEMDLLQQAKDDALELLSRDPTLRDPEHRHLRAALQSQIGEAL
                     ALAQIG"
     gene            1742..3013
                     /locus_tag="VLI90_19440"
     CDS             1742..3013
                     /locus_tag="VLI90_19440"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HSV16446.1"
                     /translation="MTRATARLAIAIVSILLGVQTKVQAADTDRAAPNVLQWDGVVLA
                     KLREQWRAHDPQVMPVLTALRDGADRALADGPYSVTFKKHPLPGVDPHDYVSLAPYAW
                     PNPDTRDHLPYVTVDGKRNPEFAEFDALPFIQITDHLEILSLAGYITGDVKYFDRAAL
                     LIRTWCIDPTTRMNPNLDHAQVIKGQNNNVGNNTGVIESRRFISFIDATELMRGTASW
                     STDDDAQFRAFISDFRTWLLSSKLGTGEAAAKNNHGSWYDVQAASYCIFRGDPAKAKS
                     IVEDAKTKRIAVQIQPHGMQPDEIRRTKSSWYCAFNLAALTQLATLGQRVDANLWTYQ
                     TDDGRSIRKAIDWLIPYYTGKKSWTVKDIEGFSGKELFIPLRQAATAYHDARYDGAAD
                     QLVGQDVEQDDEREHPLRPVGLKQLLFPARK"
BASE COUNT          637 a          967 c         1077 g          552 t
ORIGIN      
        1 ggtgaaggtt taccgcaacc tgccgcagat gacgcatccg acctgggaga cgatcgagca
       61 gggaacgccg cgggtggagc aatcgaagtt cctcgcgatc tacccggcgt cgatgcggct
      121 ggcgagcgat gtgatcgcca agctcgtcga agacaacatc aacgccgcgc tgggcgaagt
      181 gcacgagtgg ttcgacccgc agctgcttaa attgcgcggc ctgctgggcc gccgcgaggc
      241 gtatcggctc atccactgcc cggcgaacct gcgcgaagcc gtgcaggccc ggcggcggtt
      301 gatttacgac gagctgatgc tgatgcagtt gggcctgggc ctgagcaagc gactgcgcag
      361 cggccgattg accgcgccag tgatgcgcat cgacaagatg ctcgacgagc gcatccgcaa
      421 gcgatttccg tttcagctga cgaccgcgca gcagaacgct atctgggaga tccttcacga
      481 tctgcaaagc ggtcagccga tgaaccgcct gctgcagggc gacgtcggca gcgggaagac
      541 tgttgtcgcg ctgtacgcca tgctggtggc cgtcgccaat cgcatgcagt cggcgttgct
      601 cgcgccgacc gaagtgctgg cggagcagca ttacctcacg ctgtcgaact tgctgaaaga
      661 ttcgaacgtg acggtcgagc tgtacaccgg gcgaaccaag cggcaatcgc gcagcggcat
      721 cgcggggctg agcaagggaa ccgtgcatct ggcaatcggc acgcaggcgc tgatccagga
      781 agacatcgag ttcgccaacc tcggcctggt cgtcgtcgat gaacaacaca agcttggcgt
      841 gcagcagcgc gcggtgttga agagcaaggg gctcgcgccg cattacctgg tgatgacggc
      901 aacgccgatc ccacggacgc tggcgctgtc gtacttcgcg gactttgatg tcagctcgat
      961 caacgaactg ccgccggggc gtcagccgat caagaccaag ctggtgcgat cgcgcgaggc
     1021 ggccaaggcg tacgatttcg tccggcagca ggtggccaag ggccggcagg cgtacatcgt
     1081 gctgccgcgg atcgatgatg acgggctcga cgatgccaag agcgtgatga aggaattcga
     1141 tcggctgagc aaaggcccgc tgtcgggcct tcgcctggcg gtgctgcacg ggcagatgag
     1201 cactgaggaa aagcaagcga cgatgctggc gtttcgcgat cggcagaccg atgtgctggt
     1261 ggcgacgacg gtgatcgagg tcggcatcga cgtgcccaat gcgacggtga tggtgatcgg
     1321 cgacgccgag cgatttggtc tgtcgcagtt gcatcaattg cgcggccggg tgggccgggg
     1381 aacggaaata tcgtattgcc tgttgatgtc cgatgccgtg ggtgaaccgg cggagtcgcg
     1441 gctgggggcg atgacttcga ccaacgacgg gtttgaaatc gcagagctgg acctgaagct
     1501 gcgcgggccg gggcagttct tcggcacgcg acagcacggg ctgccagaat tcaagctggc
     1561 cgacatcacc accgaaatgg atttgctaca gcaggcgaag gacgatgcgc tggagctgct
     1621 aagccgtgac ccgacgctgc gcgaccctga gcaccgccac ctgcgggccg cgttgcagtc
     1681 gcaaatcgga gaagctttgg ccttggccca gatcggttga cgcgttatca attcgcccac
     1741 tatgacccgc gcaaccgcac gcttggcgat cgcaattgtt tcgatcttgc ttggagttca
     1801 aaccaaagtg caagccgccg acaccgaccg cgccgcgccg aatgttttgc agtgggatgg
     1861 cgtcgtgctg gcaaagctgc gcgaacagtg gcgggcgcac gatccgcagg tgatgccggt
     1921 gctgacggcg ctgcgcgacg gtgccgatcg cgcgctggcg gacggtccgt actccgtcac
     1981 gttcaagaag cacccgctgc ccggtgtcga tccacacgat tatgtaagcc tggcgccgta
     2041 tgcctggccg aacccggaca cgcgggatca tctgccgtac gtcacggtcg atggcaagcg
     2101 caatcccgag ttcgccgagt tcgacgcgct gccgttcatt cagatcacgg accacctgga
     2161 gatcctgtcg ctggccggct acatcacggg cgacgtgaaa tatttcgatc gcgcggcgct
     2221 gttgatccgc acctggtgca tcgatccgac gacccggatg aacccgaacc tcgatcacgc
     2281 gcaggtgatc aagggccaga acaacaacgt cggcaacaac accggcgtga tcgaatcacg
     2341 ccgattcatc agcttcatcg acgcgacaga attgatgcgc ggcacggcat cctggtctac
     2401 cgacgatgac gcccaattcc gcgcgttcat ctccgatttc cgcacctggc tgctgagcag
     2461 caagctgggc accggcgagg ccgccgcgaa gaacaaccac ggctcgtggt atgacgtgca
     2521 ggcggcgtcg tactgcatct tccggggcga tccagcgaag gcgaagtcga tcgtggaaga
     2581 cgcgaagacg aagcggatcg cggttcagat tcaaccgcac ggcatgcagc cggatgaaat
     2641 ccggcggacg aagtcctcct ggtactgcgc gttcaatctg gcggcgctga cgcaattggc
     2701 gacgctcgga cagcgggtgg atgcgaatct gtggacctat caaacggacg acggccgcag
     2761 catccgcaag gcgatcgact ggcttatccc gtactacacc ggaaagaagt cgtggaccgt
     2821 taaagacatc gagggcttca gcggcaagga gttattcatc ccgttgcgcc aagcggcgac
     2881 ggcgtatcac gacgcgaggt acgacggcgc tgccgatcag ttggtcgggc aggacgtgga
     2941 gcaggatgat gagcgcgagc atccgctgcg cccggtgggg ttgaagcagt tgctgtttcc
     3001 ggcgcggaag tgaggagccg actcacgccg ggttcggagg tactccggcg accccgatgc
     3061 tttgcttgag ccacagcaga agaatccggg tggtcgcgag tacgctccga acccggcgtg
     3121 ggcgcaaact tcaattcctc ttccgcccgc cgtggtcgcg cgcatcggca gcatcgggcg
     3181 tgccttcgat gccgacgtcg ccaccaaccg tgccgccggt ctgtgccggc gtc
//
LOCUS       DATIMH010001127         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4600054, whole genome shotgun sequence.
ACCESSION   DATIMH010001127 DATIMH010000000
VERSION     DATIMH010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4600054"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VLI90_19445"
     CDS             complement(<1..188)
                     /locus_tag="VLI90_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16447.1"
                     /translation="MTKNALRNRRDDRRLPRAATAAVMEMMERRTLLSATFANDTLTV
                     TGTSGNDNIRVDVSRNAD"
     gene            708..1205
                     /locus_tag="VLI90_19450"
     CDS             708..1205
                     /locus_tag="VLI90_19450"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSV16448.1"
                     /translation="MQYRDACAVPSLAVLLCLLTTGCQSNCPHGSDQAHRAITPDQIQ
                     WQNNPPSLPPGAKMAVLEGDPSKPGFFTIRAVFPDGFRIPPHMHPNAERVTVLSGTLY
                     LAHGEKFDESAAQALPAGSYSSMPAGMHHYGFAKGETTIQVSSIGPWGITYLNPSDDP
                     RKPVK"
     gene            1457..1765
                     /locus_tag="VLI90_19455"
     CDS             1457..1765
                     /locus_tag="VLI90_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16449.1"
                     /translation="MKLLVHLAIFAVGAALGVWWGVNHPTQAADVAGREDVAINRVKA
                     EVAKAKIELLEHFLATSKATPAPTATAAPTAPPAGSADYQKMLVQEKQNLTQAQQTTN
                     "
     gene            1929..>2116
                     /locus_tag="VLI90_19460"
     CDS             1929..>2116
                     /locus_tag="VLI90_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16450.1"
                     /translation="MSVAAADGRDAKPPLSDHHRLYFPTFSPDGRTIYFITETPGGDD
                     GISKVNTDGSGVTALKLVA"
BASE COUNT          439 a          724 c          581 g          372 t
ORIGIN      
        1 atgtcggcgt tcctgctgac atcgacgcga atgttgtcgt tgccgctcgt accggtgacg
       61 gtcagcgtgt cgttggcgaa cgtcgcgctc agcaacgtgc ggcgctccat catctccatc
      121 accgccgcgg tggcagcgcg cggcagccgt cgatcatccc gtcgatttcg aagtgcgttc
      181 ttcgtcattt caaactcctt aaaacaccat gtttgtcaga ctctgtgtgt ggcgcgcgcg
      241 caaccatgag aacgattacg ccgggcaatg cgcggggcgc accaacgacc ttggctggac
      301 atgggaaaaa accatcaaag aaaagtgggg ccttcaaatg cggcggccgg ccgacatgac
      361 gacccccgtc taccgcatca atgacttggc ttgctggcat ccctccttca gggtgaccga
      421 accaagggcc cgacaactga agcaaagcct atcgtagcca agtgcggatc gcctgaatat
      481 ccgcattttc acggatatta ccggacgatc cggcagccgt gacgctgcgt cttggcgctt
      541 ggtgcaaatc cgttcgccag ccatgcttgt cacctgattt gaactgcctg atttccgtcg
      601 ctgaatccgc atttccccaa gccgaacaca aaacattgac agatcgatca ccgcatttgc
      661 agaatgctca accagtctcc gccgtgtttt cgaatcagga aatccacatg caatatcgcg
      721 acgcctgcgc tgttccctcg ctcgccgtgc ttctctgtct tctcaccacc ggctgccaat
      781 ccaactgccc gcacggcagc gatcaagcgc atcgcgcgat cacgcccgat cagatccaat
      841 ggcagaacaa tccaccaagc ctgccgcccg gcgcgaagat ggctgtgctg gaaggcgacc
      901 cgagcaagcc cggtttcttc accatccgcg ctgtgttccc cgacggtttc cgcatcccgc
      961 cgcacatgca ccccaacgcc gaacgcgtca ccgtcctctc cggcacgctc tatctggcgc
     1021 acggcgagaa gttcgacgaa tccgccgccc aagcgctccc cgccggttca tacagctcga
     1081 tgcccgccgg tatgcaccac tacggcttcg ccaaaggcga aacgacgatt caagtcagca
     1141 gcatcggccc gtggggcatc acctatctca atccaagcga cgatccgcga aagccagtaa
     1201 aatgactggt cctttgagtg ctcgacgctc cagcccactt caccgccgag accgagcacg
     1261 ccgagatgaa cgccgaggtg aacgccagga agcgttgcgg tgaaaccggc cctcggcgtc
     1321 tgcctctgcg tcctcggcgc ctcggcggtg aactgggcgc agagaactaa aaggcgcgcc
     1381 aaatgaccta ccgactggtt gccgacacga cccttcagta aaatatccgc gggctaccta
     1441 ccggaagggc acacaaatga aactgctcgt tcacctggcg atcttcgccg tcggcgccgc
     1501 gttgggcgtc tggtggggcg tcaaccatcc cacccaggcc gccgacgtcg ccggccgcga
     1561 ggacgttgcg atcaaccgag tcaaagccga agtcgccaag gcgaagatcg agctgctcga
     1621 gcatttcctc gccacgtcca aagcaacgcc agcgcccacc gcgaccgccg cgccaaccgc
     1681 gccgcccgcg gggagcgcgg actatcaaaa gatgctcgtc caggaaaagc agaatctgac
     1741 gcaggcgcag cagacgacga attgatcgcg ccggactccc agcgcgccca ttcccacgcg
     1801 tagcggtagg cccgcagggg catgtgtttt tccgttcgaa ggcgaaaaca cgtgcgcctg
     1861 cggggcgacc gctacgccgc agaagtcttc ttcacaagcc cgacgcttgg ccaacgcgcc
     1921 gttaccaggt gtctgttgcc gccgccgacg gccgggacgc caagccgccg ctcagcgacc
     1981 atcaccggct gtacttcccc accttctcgc cggatgggcg gacgatctac ttcatcaccg
     2041 aaacgcctgg tggcgatgac ggcatttcca aagtgaacac cgacggcagc ggtgtgaccg
     2101 cgctgaagct ggtcgc
//
LOCUS       DATIMH010001128         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2007522, whole genome shotgun sequence.
ACCESSION   DATIMH010001128 DATIMH010000000
VERSION     DATIMH010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007522"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /locus_tag="VLI90_19465"
     CDS             <1..1603
                     /locus_tag="VLI90_19465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV16451.1"
                     /translation="AAKTNFLANMSHEIRTPMTAILGYADLMLAPQQTMSDRVNCLHV
                     IRRNAKHLMELINDILDVSKIEADKMTVESIECDVARVIVDVSSMLRPKAMAKRLSLA
                     VHFAGEIPRMIRTDPLRLKQILMNLVGNAIKFTENGEINVTISAQRSDHGGVLRVDIK
                     DTGIGMSSAQMQRLFKPFQQADNSMSRKYGGTGLGLVISKRLAKLMGGDVTVTSTPGH
                     GSMFTVTVDTGSLDGVEWASGLTESMLQPVTAAEAADEINLRGRILLAEDGYDNQQLI
                     SLHLTNAGADVVIAENGKIAVEKVRTEQFDVVLMDMQMPELDGYGATSELRRRGFMLP
                     IIALTAHAMTGDRTRCLDAGCTDYLTKPVDAELLLKTVQSYLLKKGAAGAAAAPMPSV
                     AQETASPAAPPAPVAVAQNDEARVVSQAAIPRSEKAAEAMRRAIGGFVERLPERVASI
                     GRLLAAHELDELKRTLHQLKGAGAGFGFPSITQLAAKAEQTVATNQALEQIRSEVDSL
                     VALIRRVDGYNPGREEYVKADAAGY"
     gene            1575..>2190
                     /locus_tag="VLI90_19470"
     CDS             1575..>2190
                     /locus_tag="VLI90_19470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV16452.1"
                     /translation="MLKQTLLVIDDSQDIHPLVGVWLMDELIDIHSAFDGVGGLERAR
                     AIIPDLILLDVDMPGTNGFEVCAQLKADPITREIPVVFLTGASDANEKLRGLELGAID
                     YIIKPFDPAELRARVRVSLRTKHLLDLLAQKAVILQESEERFRVLAENSSDIISRHNA
                     AGEYQYVSPAAQTVLGYDPQELMGRPLSVFIHPDQAALVEVWQEA"
BASE COUNT          422 a          658 c          757 g          353 t
ORIGIN      
        1 cgccgctaag accaatttcc tggccaacat gagccacgaa atccgcacgc cgatgacggc
       61 aatcctcggc tacgccgact tgatgctcgc gccgcagcag acgatgagcg accgcgtcaa
      121 ctgcctgcac gtcatccgcc gcaacgccaa gcatctgatg gaattgatca acgacatcct
      181 ggacgtgtcg aagatcgaag cggacaagat gacggtcgag agcatcgaat gcgatgtcgc
      241 ccgcgtgatc gtggacgtca gcagcatgct gcggcccaag gcgatggcca agcgactgtc
      301 gctggccgtg cacttcgcgg gggaaatccc gcgcatgatc cgcaccgatc cgctgcggct
      361 caagcagatc ctgatgaacc tggtcggcaa cgcgatcaag ttcaccgaga acggcgagat
      421 caacgtgacg atcagcgcgc agcgcagcga ccacgggggt gtgctgcggg tggacatcaa
      481 ggacaccggc atcggcatgt cgtcggcgca gatgcagcgg ctcttcaagc cgttccagca
      541 agcggacaac tcgatgtcgc gcaagtacgg cggcaccggg cttggcctgg tcatcagcaa
      601 acgcctggcg aagctgatgg gcggcgacgt caccgtcacc tccacgccgg ggcatggcag
      661 catgtttacg gtgacggtgg acacgggctc gctcgacggc gtcgaatggg ccagcgggtt
      721 gaccgaatcg atgctgcagc cggtcaccgc ggcggaggcg gcggatgaga ttaacctgcg
      781 cggccgcatc ttgctggccg aggatggcta cgataatcag cagttgattt cgctgcacct
      841 gaccaacgcc ggggcggacg tggtgatcgc cgagaacggg aagatcgcgg tggaaaaggt
      901 gcgcaccgag cagttcgacg tcgtgctgat ggacatgcag atgccggaac tggacggcta
      961 tggggcgacc agcgaattgc gtcgccgcgg gtttatgctg ccgatcatcg cgctgacagc
     1021 gcatgccatg accggcgatc gcacgcggtg tctcgatgcc ggttgcaccg attacctcac
     1081 caagccggtc gatgcggaac tgctcttgaa gaccgtgcag tcgtacctgc tgaagaaggg
     1141 cgctgcgggg gcggcggctg cgccgatgcc gagcgtggca caggagacgg catcgcccgc
     1201 ggcgccaccg gcgccggtgg cggtcgcgca gaacgacgag gcgcgcgtcg tcagccaggc
     1261 cgcgatcccg cgctcggaaa aggcggccga ggcgatgcgc cgcgcgatcg gcgggttcgt
     1321 cgagcgcctg ccggagcgcg tggcctcaat cgggcgattg ctggctgcgc acgagctgga
     1381 tgagctgaag cgcacgctgc atcaactcaa aggggccggg gcggggtttg gtttcccgtc
     1441 gatcacgcag ctggccgcca aggcggagca gaccgtcgcg acgaaccagg cgctggagca
     1501 gattcgttcg gaagtggatt cactggtcgc gttgatccgg cgcgtggacg ggtacaaccc
     1561 cggcagggag gaatatgtta aagcagacgc tgctggttat tgatgactcg caggacatcc
     1621 acccactggt gggcgtctgg ttgatggacg agttgatcga catccacagc gccttcgacg
     1681 gcgtcggtgg gctcgagcgg gcgcgcgcga tcattccgga cctgatcctg ctcgacgtcg
     1741 atatgcccgg caccaacggg ttcgaagtct gcgcgcagct gaaggccgac ccgatcacgc
     1801 gggaaatccc cgtggtcttc ctgaccggcg cttcggacgc caacgagaag ctgcgcgggc
     1861 tggagctggg ggcgatcgat tacatcatca agccgttcga tccagcggaa ctgcgcgccc
     1921 gcgtgcgggt gtcgctgcgc accaagcatc tgctggacct gctggcgcag aaggccgtca
     1981 tcctgcagga aagcgaagag cgcttccgcg tgctggcgga gaactcttcg gacatcattt
     2041 cgcgccacaa cgccgcgggg gaatatcagt atgtatcgcc cgcagcccag acggtgctgg
     2101 gatatgaccc gcaggagttg atgggccggc cgctgagcgt attcattcat ccggatcagg
     2161 cggcgctggt ggaagtgtgg caggaagccg
//
LOCUS       DATIMH010001129         5287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2868455, whole genome shotgun sequence.
ACCESSION   DATIMH010001129 DATIMH010000000
VERSION     DATIMH010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2868455"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VLI90_19475"
     CDS             <1..590
                     /locus_tag="VLI90_19475"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSV16453.1"
                     /translation="ACRQLATWKQAHRAAAAALSMSVNISARQLSTPDLVDRIKQIFI
                     DTGVSASSIILEITESVMIHNADAAIAVLEQLTALGVRFHMDDFGTGYSSLSWLHRLP
                     LSGLKIDKSFVQLMGERADYAAVVNGIVGMAKSLGMSLVAEGIETAAQATMLQRMACE
                     KAQGYFFNRPMTSTDAAAYLLRATGANQTTNAAAA"
     gene            633..1697
                     /locus_tag="VLI90_19480"
     CDS             633..1697
                     /locus_tag="VLI90_19480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HSV16454.1"
                     /translation="MRSNAHAEGCGAMHAMVLKSTAPMADRPEPLVWSQCPDPAPAAG
                     EVRIDVSVCGVCHTELDEIEGRLPPATLPIILGHQAVGRVAAVGDGAEGLKAGDRVGV
                     AWINWACGVCEFCRRGDENLCLRFRATGRDVNGGYAEQMTVPAAFAHAIPDALADVDA
                     APLLCAGAIGFRSLQLCDLRDGEPLGLTGFGASAHLVLQLARHRLPNSPIFVFARSEN
                     ERGFARELGAAWAGNTTQRAPQPLHAIIDTTPAWTPVVQALSNLERGGRLIINAIRKE
                     NADKDALLKLDYARHLWHERQIKSVANVTRNDVREFLRAAAEIPLRPQVQEYALPDAN
                     QALADLKHGDNRGAKVLRVR"
     gene            2426..2512
                     /locus_tag="VLI90_19485"
     tRNA            2426..2512
                     /locus_tag="VLI90_19485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2460..2462,aa:Ser,seq:tga)
     gene            2603..2929
                     /locus_tag="VLI90_19490"
     CDS             2603..2929
                     /locus_tag="VLI90_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16455.1"
                     /translation="MLIFSCVAIAAGGDAVAAAHTRPFSGVIGVVTKVDPLSISIQEL
                     SPTTRPATPAERTFRFVDGSRGVPAERSALRVGQNVMIGVEGDAIGFVTIMPDAKSGP
                     RAATAP"
     gene            complement(3009..4937)
                     /gene="sppA"
                     /locus_tag="VLI90_19495"
     CDS             complement(3009..4937)
                     /gene="sppA"
                     /locus_tag="VLI90_19495"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSV16456.1"
                     /translation="MKFRTACCLLLLSLFAASSATFAKSTTRPADIAAAAPTTGPTTR
                     QSAVATSKPANVSTTIHDLAQKIRQISEDQEKLPKVAYFDLSRPLIEKAPDFSLFGDS
                     DALTLRALLQRLHHARDDKAVRAVLLTLGDPGVNLAQAQEIRDALVDIRRAGKQTFVY
                     ADGYDTDSYTIATGATNICLLSGGEMMLPGVGIETMYLKGLFDKLGVKADYVQIGEYK
                     GADEEYTRTTASEEFRGELNKLMDSLYAQVVDGISLNRNLSREQVKSVIDESILTAQV
                     AKDRGFIDHLVDADGLRDLIAQEIGSKVELVKDYDAPQREQVDLSSPFALFSLMMKKP
                     EASTKPAVALVYADGVIVDGEGGGSLFGGATIGSDAIRRAMRVATRDETIKAVVIRIN
                     SPGGSALASEAMWQAVRRVAKDKPVIISVGSMAASGGYYLASAGDRIFADSSAIVGSI
                     GVVGGKFVIKDLYEKLGLTTESFTRGRNADLFSSNTEFSDRQRRMVTNWMKQTYDQFT
                     QRVMTTRAGKIKDIDQVARGRVFIAQQAKDLGMVDEIGGVERAIAYAADQAQLKPEDY
                     DVRIVPAPKTLADYINGSAAEETASPIQPRIELGEMTLLKAMPPMVRSMVVEQLQMMQ
                     LLQQRPILLAAPFALRVN"
BASE COUNT          902 a         1690 c         1725 g          970 t
ORIGIN      
        1 aagcctgccg gcagttggct acctggaagc aggcccatcg cgccgcggca gcggcgcttt
       61 cgatgagcgt caacatctcc gcccgtcagc tatcaacacc ggacctggtt gatcgcatca
      121 agcagatctt catcgatacc ggcgtcagcg cttcgtcgat catcctggag atcaccgaaa
      181 gcgtgatgat ccacaacgcc gacgccgcca ttgccgtgct cgaacaactg acggcgctgg
      241 gcgtgcgctt tcacatggac gacttcggca cgggttactc gtcgcttagt tggctgcacc
      301 gcctgccgct gagcgggctg aagatcgaca agagcttcgt gcagctgatg ggcgagcgcg
      361 ccgattacgc ggcggttgtg aacggcatag tcggcatggc caagagcttg ggcatgtcgc
      421 tggtggccga gggaattgaa accgccgccc aggcgaccat gctgcagcgc atggcctgcg
      481 agaaagcgca aggctacttc ttcaatcgcc cgatgacctc aaccgatgcc gcggcgtatc
      541 tgctgcgcgc taccggcgcc aatcaaacta ccaacgccgc cgcggcgtag acgattcgcg
      601 cgcacatgcg cccacgccta caatcctgcg gcgtgcgatc gaacgcgcac gcggaaggat
      661 gcggcgcgat gcatgcgatg gtgctgaaga gcacggcccc gatggcggat cgacccgagc
      721 cgctggtttg gtcccagtgc ccagaccccg cgccggccgc gggggaagtt cgaatcgacg
      781 tcagcgtctg cggcgtttgc cacaccgaac tggatgaaat cgaaggccgg ctgccgccgg
      841 cgacgctgcc gatcatcctg ggccatcagg cagttggacg cgtggccgcc gtgggtgacg
      901 gcgccgaagg actcaaagcc ggcgaccgcg tcggcgtcgc gtggatcaac tgggcgtgcg
      961 gcgtctgcga gttctgccgc cgcggcgacg agaatctctg tcttcgcttc cgcgccaccg
     1021 gccgcgacgt caacggtgga tatgccgagc agatgaccgt gcccgcggcg ttcgcgcatg
     1081 cgattcccga cgcccttgcc gatgtcgatg ccgccccgct gctgtgcgcc ggcgcgatcg
     1141 gctttcgatc gctgcagctt tgcgacctgc gggacggcga gccgctcggc ctgacgggct
     1201 tcggcgcatc ggcacacctg gtcctgcaac tggcccggca tcgcctgccg aattcgccga
     1261 tcttcgtctt cgcgcgctcg gagaatgagc gcggcttcgc gcgcgaactg ggcgccgcgt
     1321 gggcaggcaa cacgacgcag cgcgccccgc aaccgctgca cgcgatcatc gacaccacgc
     1381 ccgcatggac gccggtcgtg caggcattgt caaacctcga acgcggcggc cggctgatca
     1441 tcaacgcgat ccgcaaggag aacgccgaca aagatgcgct gctcaagctg gactacgcca
     1501 ggcacctgtg gcacgaacgc cagatcaagt ccgtcgccaa cgtcacgcgc aatgatgtcc
     1561 gcgaattcct ccgcgccgcg gcggagattc cgctgagacc ccaagtgcag gaatatgcgc
     1621 tgcccgacgc gaaccaggcg ctggccgatc taaagcacgg cgacaaccgt ggggccaaag
     1681 tcctgcgcgt gcgttgaaaa ttggacatgg cgccggcccc gcccgtgcgt catgggcagt
     1741 gcattgcaaa actagaatgg aaatgcaaat gcaaccgccg agggcgccga gcacgccgag
     1801 acagacgccg agaggaggaa agagcgatgg ctttaagggg gctttagaac cttcaacatg
     1861 acacgggcct ccggctgcct ccggcccgtg cgtgacgctt tggttagtcg cagagccgtg
     1921 ggtttccgaa gctcagcgca tcgcgcacgg gccggaggcc cgtgtcacgt tgaggctgcc
     1981 aatgttctga aaccgcttct ctgcggtacc aacttttgtc ttctcggcgt ccgcctcggc
     2041 gtgctcggcg ccctcggcgg ttgcattgca aggccgtatc accgtcggtc accgctcgtg
     2101 ggcatcaccc gtctttggtc aatacgaccg cggtgggatc gtcgggtctt aataggaaga
     2161 tcgacatggc gtgggcgtaa agcgggatcg tcaacgccat caaagccacg atcgctgtac
     2221 gcaagattgt ctcccagccg tcgcgcgagc tctttcaccg actggacgtt cgaagcgtaa
     2281 cgtatgcgcg ctgctcggat caacgggatt caccatacgc taaccgctga agcggttagc
     2341 ccgggcggag cgtcggcgcg cgtcatcgct tcgttcgccg ttttgaccgc tctggatggg
     2401 gcggataaac tttcgcgggc tccccggagg agtggcagag cggttgaatg cgccggtctt
     2461 gaaaaccggt tggcccgcaa gggtcacggg ggttcgaatc cctcctcctc cgctgtttat
     2521 cacacgtctg cacgcacgcg cgagggcgcg cgagggtctt cacatgtgaa ttatctcctg
     2581 cgctatcgcc gcggctttgg tgatgttgat cttcagctgc gtcgccattg ccgcgggcgg
     2641 tgatgcggtt gcggcggcgc acacgcggcc gttttccggt gtgatcggcg tggtgacgaa
     2701 ggtggatcca ctgtcgattt cgattcaaga gctgagccca acgacgcgac cggcgacgcc
     2761 ggcggagcgg acgtttcgct tcgtggacgg ctcgcggggg gtgccggctg agcggagcgc
     2821 gctgcgcgtg ggacagaacg tgatgatcgg agtcgaaggg gatgcgattg gcttcgtgac
     2881 catcatgccg gatgcgaaga gcggaccgcg agcggcgacg gcgccgtgat aggttaagga
     2941 gagccggccc gtcctcgggc cggaatcgag cgcggaccgg gccggggacg gcccggctct
     3001 cctgctgttc aattcacccg cagggcgaac ggggcggcca gcaggatcgg acgctgctgc
     3061 agcaactgca tcatctgcaa ctgctcgacc accatcgatc gcaccatcgg cggcatcgct
     3121 ttcagcaaag tcatctcgcc caattcgatc ctgggttgga taggcgaagc ggtctcctcg
     3181 gcggcgctgc cgttgatgta gtcggccagc gtcttaggcg cggggacgat gcgcacgtcg
     3241 taatcttccg gcttcaactg tgcttgatcg gccgcatagg cgatcgcgcg ttcgacgccg
     3301 ccaatctcgt ctaccatccc caggtccttc gcctgctgcg cgatgaagac gcggccgcgc
     3361 gccacctggt cgatgtcctt gatcttcccg gcgcgggtcg tcatcacgcg ctgcgtgaac
     3421 tggtcgtagg tttgcttcat ccagttggtg accatgcggc gttggcggtc gctgaattca
     3481 gtgttggagc tgaacaggtc cgcgttgcgg ccgcgggtga acgactcggt tgtcaggccc
     3541 agcttttcgt acaggtcctt gatgacgaac ttcccgccga cgacaccgat ggaaccgacg
     3601 atggcggagg agtcggcgaa gatgcggtcg ccggcgctgg cgaggtaata acctccgctg
     3661 gcggccatcg aaccgacgct gatgatgacc ggcttgtcct tggccacgcg gcggacggct
     3721 tgccacatcg cctcgctcgc cagcgcgctg ccgccggggc tgttgatgcg gatgacgacc
     3781 gccttgatgg tctcatcgcg cgtcgcgacg cgcatcgccc ggcggatggc gtcggacccg
     3841 atcgtcgccc cgccgaacag gctaccaccg ccttcaccat cgacgatcac accgtcggcg
     3901 tacaccagcg ccaccgcggg cttggtcgat gcctccggct ttttcatcat cagcgaaaag
     3961 agcgcgaacg gactcgacag atcgacctgc tcgcgctgcg gcgcatcgta gtccttcacc
     4021 agctcgacct tgctgccgat ctcctgcgcg atcagatcgc gcaggccgtc ggcatcgacc
     4081 aggtgatcga tgaacccacg gtccttggcc acctgcgcgg tcaggatcga ttcgtcgatc
     4141 accgacttca cctgctcgcg cgacaggttg cggttcaagc tgatgccatc gaccacctgg
     4201 gcgtacagcg aatccatcag cttgttcaac tcgccgcgga attcctcgct ggcggtggtg
     4261 cgggtgtatt cctcgtcggc gcctttgtat tcgccgatct gaacgtagtc cgccttcacg
     4321 ccgagcttgt cgaacaggcc cttgaggtac atcgtttcga tgccgacgcc cggcagcatc
     4381 atctccccgc cgctgagcag gcagatgttg gtggcgccgg tggcgatcgt gtagctgtcg
     4441 gtgtcgtagc cgtcggcgta gacgaaggtc tgcttgccgg cccggcggat atcgacgagc
     4501 gcgtcgcgaa tctcctgcgc ctgcgcgaga ttgacgccgg ggtcgccgag tgtcagaagg
     4561 acggcgcgca ccgccttgtc atcgcgggcg tgatgcaggc gctgcagcag cgcccgtagc
     4621 gtcagggcat cgctgtcgcc gaagagcgag aagtcgggcg ccttttcgat cagcggccgg
     4681 ctcaggtcga agtacgcgac cttcggcaac ttctcctggt cttcgctgat ctggcgaatc
     4741 ttctgggcca ggtcgtggat ggtcgtcgag acattcgcgg gcttgctggt ggccaccgcg
     4801 gattggcgag tggtcggtcc cgtcgtcggc gccgcggcgg cgatgtcggc gggtcgcgtg
     4861 gtggatttgg cgaacgtcgc gctcgaggcg gcgaacagcg acagcaataa aagacagcag
     4921 gcggtccgga acttcatggt ggctcctcaa tcaacagccg aaatgttcga gcgattccag
     4981 cacggcgcga atctcgttct atcggcacaa gcatagccgc gaattgacgc ttctgccgcc
     5041 acttttataa tgccgcccgt caatcaccga accgcaacgc ggcggacggg aacaaccggg
     5101 gaaggagacc cctctggtgc gcgtccgcgg cttcgttatc accctgatca gcctcgggtt
     5161 gctcgcctcg ctcaacgccc ccgccgctcg cgctcagcag tcgcagctgc ccgcggggtc
     5221 gatgttgatc aattctgcgc aggcgctggt ttggactgag gggcgcacca gcgtcgtcga
     5281 gctcaag
//
LOCUS       DATIMH010001130         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_267240, whole genome shotgun sequence.
ACCESSION   DATIMH010001130 DATIMH010000000
VERSION     DATIMH010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267240"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VLI90_19500"
     CDS             <1..1446
                     /locus_tag="VLI90_19500"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV16457.1"
                     /translation="LVDAMQRQQVAAIGGPNLPPPDDSWTAQCVAASPGGPSHVMLDD
                     QLAEHVPGCNMAFRRDLLLKIGGFDPQFRQAGDDVDVCWRWMDAGFRIGYAASAVVWH
                     HRRNTVRAFLRQQKGYGRSEAMLLFKHPERFNRMGCSRWRGIIYGEGAVGLPTAEPLV
                     FHGRYGSGLFQTIYHRNDYSAWAYFTLLEWHTLAAFALLMSYAFAPLAALAGAMWIAT
                     LFAAARSTSGSPLSRSAPLWCRPLVFGLHIAQPVIRAVTRYTYRLSRKQLPQMPADGP
                     RDAAPLKRISATVRDAYWASRRGRGREEFLKALEAEAHEHGWRGLFHEEWRSWDVILL
                     GDRWHNIMLHSATEELGGPKRFTRVRCTLQMTAFAAVVCTMLLAAIVIESVDGRWPQF
                     ASAIFGALAAGLLARLYISRRRSFATLAILLGAAGRDAGLNPVDPAEDKDDGHRRRGR
                     KAVAVDDAEISDDIPADAIETEPAVMARQAL"
     gene            1476..2123
                     /locus_tag="VLI90_19505"
     CDS             1476..2123
                     /locus_tag="VLI90_19505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSV16458.1"
                     /translation="MNLNSTKASPRPRGFTLVELLVVIGIIAVLIGILLPALSKAREQ
                     SKRTACLSNLRSLGNGFIMYANTYKDRLPNESPPGTINAALIQAWADDVMVAFNTNQV
                     RSAAVFHCPADEDPIPASIATADPSLPNSALVSYNFYSLWWDSHYGPLLPRLHGKAPL
                     AWDINGGGLPRSKDQNHGVKGGNVLFSDGHAEWEDAKLWEGHDKPYPGDEFYNRY"
     gene            2130..>2821
                     /locus_tag="VLI90_19510"
     CDS             2130..>2821
                     /locus_tag="VLI90_19510"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HSV16459.1"
                     /translation="MAAGASPKSVMSKLRLLRYALPYWRGWSVIVGTMLAGVGMNVLK
                     PWPTALLVDNVLGNKPIAPNVTRALAWLPASAGREGLLLWVTIGTVIIFLIGWVVGTI
                     NGWVMLGIGQRMTYDVGADLFLHLQKLSLLFHSRRSVADSINRVNGDTYCVQTLITGV
                     IMPFVQSVVTLLAMFGIMWRLDHRLTLLSLVTVPFMMLAVKFFSQSMWQRTHERRELE
                     GRMMGIIHQALSA"
BASE COUNT          492 a          905 c          916 g          508 t
ORIGIN      
        1 ctcgtcgatg cgatgcagcg ccagcaggtg gcggcgatcg gcgggccgaa cctgccgccg
       61 ccggatgaca gctggacggc acaatgcgtc gccgccagtc ccggcgggcc gagccacgtg
      121 atgctcgacg accagctggc cgagcacgtg ccgggttgca acatggcgtt tcgccgcgat
      181 ctgctgctga agatcggcgg gtttgatccg cagttccgcc aggcgggcga tgacgtcgat
      241 gtctgctggc gctggatgga tgccggattc cgaatcggtt acgccgcgtc ggcggtcgtc
      301 tggcatcatc gccgcaacac ggtgcgcgcg tttctccgcc agcaaaaagg ctatggccgc
      361 agcgaagcga tgctgctgtt caagcacccc gagcgcttca accgcatggg atgttcgcgc
      421 tggcgcggga tcatctacgg cgaaggcgcc gtcggcctgc cgacggccga gccgctggtc
      481 ttccacggcc gctacggctc cggcttattt cagaccatct atcatcgcaa cgattacagc
      541 gcctgggcct acttcacgct gcttgagtgg cacactctcg cagccttcgc gctgctgatg
      601 tcatatgcgt tcgcgccgct ggccgcgctg gcgggggcga tgtggatcgc gacgttgttc
      661 gccgcggcaa gatcgacaag cggcagcccg cttagtcgct cggcaccgct gtggtgccgc
      721 ccgctggtgt tcggcctgca catcgcgcaa ccggttattc gcgcggtgac gagatacacc
      781 tatcgcttgt cgcgcaagca actgccgcag atgccggcgg atggcccgcg cgatgcggcg
      841 ccattgaagc gcatctccgc cacggtgcgc gatgcgtatt gggccagccg ccgcgggcga
      901 ggtcgcgagg aatttctgaa ggcactggaa gccgaagcac acgaacacgg ctggcgcggt
      961 ttgttccacg aagaatggcg cagctgggac gttatcctgc tgggcgaccg ctggcacaac
     1021 atcatgctgc acagcgcgac cgaagaactg ggcggaccga agcgattcac ccgcgtgcgc
     1081 tgcacgctgc agatgaccgc cttcgcggcc gtcgtctgca cgatgctgct ggcggcgatc
     1141 gtgatcgaat ccgttgacgg ccgctggccg caattcgcat ccgccatctt cggcgcgctt
     1201 gccgcgggcc tgctcgctcg cctgtacatc agccggcggc gatcgttcgc caccctcgcg
     1261 atattactcg gcgccgccgg gcgcgacgcg gggttgaacc cggtcgatcc cgcggaagac
     1321 aaggacgacg gccatcgccg gcgcgggcga aaagctgtcg ccgttgacga cgcggagatc
     1381 agcgacgaca tccccgccga tgcgatcgaa accgaaccgg cggtgatggc ccgacaagca
     1441 ctgtgaccgc gatcgtcgaa gaaggagagt gagctatgaa tttgaattca accaaagcgt
     1501 ccccgcgacc ccgcggcttc accctggtgg agctgctggt cgtcatcggc atcatcgccg
     1561 tgctgatcgg catcctgctg ccagcgctga gcaaggcgcg cgagcaatcc aagcgcaccg
     1621 cgtgcctgtc gaacctgcgg tcgctgggaa atgggttcat catgtacgcc aacacgtaca
     1681 aggaccgcct gccgaacgaa tcgccgcccg gcacgatcaa cgccgcgctg atacaggcgt
     1741 gggccgacga cgtgatggtc gcgtttaata cgaatcaggt gcgcagcgcc gcggtcttcc
     1801 actgccccgc ggacgaagat ccgattcccg ccagcatcgc cacggcggat ccgtcgctgc
     1861 caaattccgc gctggtaagc tacaacttct actcgctctg gtgggattcc cattacggcc
     1921 cgctgctgcc tcgcctgcac ggcaaagcgc cgctggcatg ggatatcaac ggcggcggct
     1981 tgccgcgcag caaggaccag aaccacggcg tcaagggcgg caacgtatta ttctccgacg
     2041 gccacgccga atgggaggat gccaagttgt gggaaggcca cgacaagccg tatccgggcg
     2101 acgagtttta caacaggtat tgattcacca tggctgcggg cgccagtcca aaatcagtga
     2161 tgtcgaaact gcgcttgctg cgctacgcgc tgccgtattg gcgcgggtgg tccgtcatcg
     2221 tcggaacaat gttggcgggc gtggggatga atgttctcaa gccctggccg accgcgcttt
     2281 tggtggacaa tgttctgggc aataaaccaa ttgcgccaaa cgttacccgc gcgctcgcgt
     2341 ggctgccggc cagtgccggc cgcgaaggcc tgctgctgtg ggtcaccatc ggaaccgtca
     2401 tcatcttcct gatcggttgg gtggtcggca cgatcaacgg ctgggtgatg ctcggcatcg
     2461 ggcagcgcat gacgtatgac gttggcgccg acctgttcct gcacctgcag aaactgtcgc
     2521 tgctgttcca cagccggcgg tccgtcgccg attccatcaa ccgcgtgaac ggcgacacct
     2581 attgcgtgca gacgctcatc accggcgtga tcatgccgtt cgtgcaatcg gtcgtcacgc
     2641 tgctggcgat gttcggcatc atgtggcggc tggaccaccg gctgacgctg ctgtcgctgg
     2701 tcaccgtgcc gttcatgatg ctggcggtca agttcttcag ccaatcgatg tggcagcgca
     2761 cgcacgagcg gcgcgagctg gaaggccgga tgatgggcat catccaccag gcgctgagcg
     2821 c
//
LOCUS       DATIMH010001131         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4313470, whole genome shotgun sequence.
ACCESSION   DATIMH010001131 DATIMH010000000
VERSION     DATIMH010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4313470"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VLI90_19515"
     CDS             complement(<1..300)
                     /locus_tag="VLI90_19515"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HSV16460.1"
                     /translation="MAYASAKPVALGLAPSFGFGDRMGMATPGHAIALMNSGTGILPI
                     FAQQSIREMARTNRQPADVMNAAAAGVKEMGYTGPQGADADHLKTPEDVNRTAAAG"
     gene            complement(452..823)
                     /locus_tag="VLI90_19520"
     CDS             complement(452..823)
                     /locus_tag="VLI90_19520"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HSV16461.1"
                     /translation="MPDKKDSPDTTATTSAAAAPAKAKPARKPKSRPLPPYNVVLLND
                     DDHTFEYVMEMLKSLFAHPHEMGMQLAQRVHREGRAVVYTTHKEKAELKRDQIHAFGT
                     DQRVACCAGSMSALIEPAQAE"
     gene            914..1585
                     /locus_tag="VLI90_19525"
     CDS             914..1585
                     /locus_tag="VLI90_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16462.1"
                     /translation="MPIRLLPIVFAVLCCTAYCTLAPACLRPSPNKRAIQWSTAIVHA
                     KLRTVAARGAGGEQYGMEVVDVLEGPLKPGETVNAKHTIPSGNRDQCAGVLTNDDAGK
                     TFLFLLRQGAHDGDWQVVTDQAIDPADPTDAEAFKQLLAETRKAEAALTDDQVKAQAL
                     ALANAEDDTEAQQAEDTLLDMGPKAVPAIRSVLESSTPIGKHRLEKIIKDVTPPTDQS
                     KTPSP"
     gene            1582..2004
                     /locus_tag="VLI90_19530"
     CDS             1582..2004
                     /locus_tag="VLI90_19530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HSV16463.1"
                     /translation="MKSHTKYLTFNIPARMGFENITPQVEQAVRDSGVREGLVLVNAM
                     HITASVFINDNESGLHHDYQKWLEQLAPFDPSPTRYQHNRTGEDNADAHMKRQIMGRE
                     VVIAITNGKLDFGPWEQIFYGEFDGWRAKRVLIKVIGE"
     gene            complement(2132..>2849)
                     /locus_tag="VLI90_19535"
     CDS             complement(2132..>2849)
                     /locus_tag="VLI90_19535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSV16464.1"
                     /translation="ANDFERELLMPATERLLALRPGERVLDACCGNGKYARRFGRIGA
                     NVVGFDGSEAFVDIARKRTTPEDGAIAYHVADAWDERAVRAIADDQSFDAALCSMALM
                     DLPGIAPLLGAIRAALRPGGRFVFSVSHPCFNSNGSTMIAELINRPDGGIEQLFGVRV
                     MKYLESTAALSNGILNQPEPNYVFHRPLGALLTACFAAGFVMDAIQEPAYPPGAGGAK
                     NAFTWKKRPQIPPVLLVRLR"
BASE COUNT          557 a          907 c          886 g          499 t
ORIGIN      
        1 ccccgcggcc gcggtgcggt tcacgtcttc cggcgttttg aggtggtcgg cgtccgcgcc
       61 ttgcggcccc gtgtatccca tctccttcac ccccgcggcg gcggcgttca tcacgtcggc
      121 gggctggcgg ttggtgcggg ccatctcgcg gatcgattgc tgcgcgaaga tcggcaggat
      181 cccggtgccg gaattcatca gcgcgatcgc gtgccccggc gtggccatgc ccattcgatc
      241 accgaaacca aagctcggcg ccagccccag cgccacgggt ttagcagatg cgtaagccat
      301 atgtgaaaat ctcctcgtca attctaaaac ccaccggcca gcgccgacgg gggccacgca
      361 tcttataaag cggcagccaa ccgcaccatc caccgttagg aaagccaaaa gtcgcgttag
      421 atttgaatag cagaagtggc gaaaattgcg atcattccgc ctgggcgggt tcgatcaggg
      481 cggacatgga ccccgcgcag caggcgacgc gctggtcggt gccgaaggcg tgaatctggt
      541 cgcgcttcag ctcggccttt tccttatgtg tcgtgtagac gacggcccgg ccttcgcggt
      601 gcacgcgctg ggccagctgc atccccatct catgcgggtg ggcgaacaga gacttcagca
      661 tctccatcac gtactcgaac gtgtggtcgt catcgttcaa cagcacgacg ttatacggcg
      721 gcagcgggcg gctcttgggt ttgcgcgcgg gcttagcctt ggccggggca gcggcggcgc
      781 tggtcgtggc ggtcgtgtct ggactgtcct tcttatccgg cataacttga cgtgcggcgg
      841 atttggaacc tcccaaaaaa ccatacagcc gcccggaaag caagaatatt ttccgcctgc
      901 gacgggaacc cctatgccca ttcgactgct gccgatcgtt ttcgccgtcc tttgttgcac
      961 cgcctattgc acactggcgc ccgcttgcct gcggccctcg ccgaacaaac gggcgatcca
     1021 atggtccacg gccatcgtcc acgccaagct ccgcaccgtc gccgcccgcg gggcaggtgg
     1081 agaacagtat gggatggagg tcgtggacgt gctggaaggc ccgctgaaac ctggcgagac
     1141 tgtgaacgcg aagcacacga tcccatccgg caaccgcgat caatgcgctg gcgtgttgac
     1201 caacgacgac gccggcaaaa ccttcctgtt ccttcttcgg cagggcgcac acgacggcga
     1261 ttggcaagtg gtgacggacc aggcgatcga ccccgccgat cccaccgatg ccgaggcgtt
     1321 caagcagctg ttggcggaaa ccagaaaagc cgaggcggcg ctgacggacg atcaagtcaa
     1381 agcccaagcc ttggcgctgg ccaatgccga agacgacacc gaagcccagc aggcggaaga
     1441 cacgcttttg gacatgggtc ccaaagcggt gccggcgatt cgctcggtgc ttgaatcttc
     1501 aaccccgatt gggaaacatc gcctggagaa gatcatcaag gacgtcacgc cgccgacgga
     1561 ccagagcaag acgccatcgc catgaagtcg cacacgaagt atctgacgtt caacatcccc
     1621 gcgcggatgg gcttcgagaa catcacgccg caggtcgagc aagccgtgcg cgacagcggc
     1681 gtgcgcgagg gtttggtgct ggtcaacgcg atgcacatca cagcctcggt cttcatcaac
     1741 gacaacgaat ccggcctgca ccacgactac caaaaatggc tcgagcaact cgccccgttc
     1801 gatccatcgc ccacgcgtta ccagcacaat cgcaccggcg aagacaacgc cgatgcgcac
     1861 atgaagcggc agatcatggg ccgggaggtc gtcatcgcga tcaccaacgg caagctggat
     1921 tttgggccgt gggagcagat cttctacggc gagtttgacg ggtggcgggc gaagcgcgtg
     1981 ttgatcaaag tgatcgggga gtgagcgagc cgtgccacgc cgggttcgga gcgtactcgc
     2041 gacgaagaga gattcttctc tcgcgctaaa aaatccgggt cgtcggagta cctccgaacc
     2101 cggcgtgggt gcagtccaca gtccgctcgt gttaccgcag ccgcaccaac aacaccggcg
     2161 ggatctgcgg gcgcttcttc cacgtgaacg cgttcttggc gccgccggcg ccgggtgggt
     2221 acgccggctc ttggattgca tccatcacga accccgcggc gaagcacgcg gtcagcagcg
     2281 cgcccaaggg acggtggaaa acataattcg gttccggctg attgaggatg ccgttgctga
     2341 gcgccgcggt tgattcgaga tacttcatga cccgcacgcc aaagagttgc tcgatcccgc
     2401 cgtcgggccg gttgatcaat tccgcgatca tcgtgctgcc gttggaattg aaacacgggt
     2461 gggagacgga aaaaacgaac cgcccgcccg gacggagcgc cgcgcggatc gcaccaagca
     2521 gcggggcgat gcccggaagg tccatcagcg ccatgctgca gagcgccgcg tcgaatgatt
     2581 gatcgtcggc gatcgcgcgc accgctcgtt cgtcccatgc gtcggccacg tgataggcga
     2641 tcgcgccgtc ttccggcgtc gttcgcttgc gagcgatatc aacgaacgct tcggatccat
     2701 cgaacccgac cacgttcgcc ccgatccggc caaaccggcg ggcgtatttg ccgttgccgc
     2761 agcaggcgtc gagcacgcgt tcgccgggcc tgagcgcgag caatcgttcc gtcgccggca
     2821 ttagcaattc gcgctcgaaa tcgttcgct
//
LOCUS       DATIMH010001132         6400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4601017, whole genome shotgun sequence.
ACCESSION   DATIMH010001132 DATIMH010000000
VERSION     DATIMH010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4601017"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VLI90_19540"
     CDS             complement(<1..591)
                     /locus_tag="VLI90_19540"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSV16465.1"
                     /translation="MTTTASASVSHDAATLLRRHGERLHEYFAQIRARHQQAPPRLVE
                     AIQYSLLAGGKRLRPTLVLECCDACLGAAGNRANADKAHASALAAAAAMEFIHTFSLV
                     HDDLPAMDDDDLRRGRPTNHKVFGEAMAILAGDAMTTLAFELIATDADAAVVGMLVKE
                     LAIASGPEGMIGGQVLDIDGENKNLSLPQLQQIHRMK"
     gene            complement(1098..3980)
                     /locus_tag="VLI90_19545"
     CDS             complement(1098..3980)
                     /locus_tag="VLI90_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16466.1"
                     /translation="MPTLNSTTAGRTLLAITFPLLVITAADCHPKPSTNATKTTPVAS
                     VSPAPKEPSSTEPSEVTTPAPVTTEPTAEATTAPTTELTAEAPVTQPANEPAVAAVTP
                     SPETTTAMPGAAAAGVAAGVTATAATEPATRPTTEATTEPTTSHTAAAAGAPPLNPAP
                     PTTNPTEIEGDRVLAEQFASIAQAAIPSKVPAPELWQPMLQQVAAILQAANKLDPTNA
                     RVGRLLADADLRLHDDEGAIDALRSVRAADPKDEFAQAQTIDLYANKMDTAEAKIAYL
                     KTIMGTPTVPDGVRAHAAVVCAKLLVDRGQEEQGKNVLGEALRANPLSGEGLRLRYQL
                     LSPTASHFERASALMELIRANPGQAAYTEMLADQFAESALVAESVPWYNLALNISART
                     GHPSISAATKQAAALFIAGDANTANQMVDNILTADPANVSIWFLKLAIVHSSAANKDD
                     FTKVLQQARNAMSNRVIELVNQIGGTTGDKPTTRPITAEGPYTLPDLRLTIAKINQDA
                     PPELKEAFTEAVSRLAMLETYFAQDPEAGSKLIDVLKQIRPDDDPAVARLEGWLFLIQ
                     GKNDEAKVKFSAIAAQDPMATLGLIRMESNKELAATLGRKLVSDYPSGLIGGILWDGL
                     RPLGVKIVTSKQADALRQIGEQFPKDFMHVLDQPQSFYAVHVDPLVVARDYDEPLLAR
                     LSIVNMGNADLTIGSEGVIRPDLWFNAQVKGIVEQSFVGTALDRVAGPMVLRSRQSTA
                     QVIRLDQGPLLQFLQNDPTVAFEVYGSVVTNPLTGPKGELSAGPGGYAVKFSKVMNRS
                     AAPPDGPAPQRAIAALQDGKPADKINAVNLLSSYVRSILSKKDVPDAVKQKTLGLVQM
                     ILKAREDRVPAVAATANYQAGRLLGGDQAKQLIQEMSRKPDWRQRVVALMVIDQLPMD
                     AQKDLATQLSSDPEPSVRELAQARVQLVDIIAKLPPSATQPAATQP"
     gene            complement(4073..4378)
                     /locus_tag="VLI90_19550"
     CDS             complement(4073..4378)
                     /locus_tag="VLI90_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16467.1"
                     /translation="MIAKLMMLSATALAAILMVASLWAWQQAPHLVFDADRPDIALWA
                     VRTTAVAAVALAQAVLLLFVVGRIYQTRGLDIVLRVLAAAVFTVALVSAIALSLAGR"
     gene            complement(4417..5241)
                     /locus_tag="VLI90_19555"
     CDS             complement(4417..5241)
                     /locus_tag="VLI90_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16468.1"
                     /translation="MISAESAIRALRRDLTVASLLKGLLVGAALLCLIAGPVVGSRFP
                     LTLTVAAIGAIWLILSYRSAKGSSLAADSPLLIASGQFDEAEHRIEQVLRTFSIFRTV
                     KLLSLHHLAVLRHAQRRWRESALLSEALLHQRLGSLQGLSRSSRLMLADSLMELGDLR
                     GAHAALVGLYNQRLTLAEAMNLLAVQLDYESRVAAWPAMVQNLPAKVQLAELMPSTTA
                     ARAQSLLALAARKTGRDDWSTWLRRRAELLVDVNALVAERPLLKELWSQETTGRLA"
     gene            complement(5447..6370)
                     /locus_tag="VLI90_19560"
     CDS             complement(5447..6370)
                     /locus_tag="VLI90_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16469.1"
                     /translation="MTVTDAADASSPTSPPPSPGYLDVPTLLELSQPHPRGNWFWFGA
                     GLFAMLVIGSTMASSRSQQMHQAVELLSLLVLFGLMGAMTMVTMLTVRQHRAQQQTVE
                     AIEELVQLRRWDQAGMALQLFLSTPARSPRARAQALVYLSSVLARHHRYEDAIAVQNH
                     LLENDLVDPPTAYGLRLGRAMAMLHEDHLVDAHQAIADLRRGGASDHSGGLALVEIYR
                     DVKTGHPTEAIESFAEKLPLMQQQLGHRVADAHALVARAYDMLGREDEARAAFERATL
                     LSPVSELTRRYAEVQKLTAKYQPAPAPPEAM"
BASE COUNT         1011 a         2005 c         2193 g         1191 t
ORIGIN      
        1 cttcatccgg tggatctgct gaagctgcgg cagcgagagg ttcttgttct cgccgtcgat
       61 gtcgaggacc tgcccgccga tcatgccttc gggcccgctg gcgatcgcca gttccttcac
      121 cagcatgccg acgaccgccg cgtcggcgtc ggttgcgatc aactcgaacg cgagcgtggt
      181 catcgcgtcg ccggcgagga tcgccatcgc ttcgccgaag accttgtgat tggtcggccg
      241 gccgcggcga aggtcgtcgt cgtccatcgc ggggagatcg tcgtgaacga gcgagaaggt
      301 gtggatgaac tccatcgccg ccgcggcggc aagggcggac gcgtgcgcct tgtcggcatt
      361 ggcgcggtta ccggcggcgc ccaaacaggc gtcgcagcat tccagcacga gcgtcggccg
      421 caggcgtttg ccgccggcca gcaggctgta ttggatcgct tcgaccagcc gcggcggggc
      481 ttgctgatgg cgggcgcgga tctgcgcgaa gtattcgtgc agccgctcac catgccggcg
      541 gagcagcgtg gcggcatcgt gggaaacaga cgcggaagcg gtcgtggtca tggccgcggg
      601 cattgtacgg aatcagccga cttgatgcga attccggctt cggcattacc ccctctccca
      661 cgtaatcgct gaagccccga cacttttgca ggaattgtcg gaaaccaacc gcgccgggtg
      721 cggaggtact ggcgacgacc cggatgcttt tcgctcgccg caggatccgg gtcgtcggag
      781 tatccttcga cttcgctcgg gacaggcctc cgaacccggc gtggagtggg ccaaggccgc
      841 ttgtacaaaa gtgtcggatc gtcagcacgt agtcgtggga gacgtctggg gttagggtga
      901 ccgttgtcaa tgaccaatgt ccaagccccg aatcccaatg aagtcacaaa cccaaatccc
      961 gacgagcgcg tagggcgcgc acctgggaat tgatgattgg aacttcattg ggattttagg
     1021 cttgcgcatt ggtcattcat cgctccaaat cacccctaac cctctcccat gattccatgg
     1081 gagaggggat cggagaatca aggctgcgtc gccgctggtt gcgtcgcgga cggcgggagc
     1141 ttggcgataa tgtccaccag ctgcacgcgg gcctgggcca gctcgcggac gctcggctcc
     1201 gggtcgctcg acagctgcgt ggcgaggtcc ttctgcgcgt ccatcggcag ctggtcgatc
     1261 accatcagcg ccaccacgcg ttgccgccag tccggcttcc gcgacatttc ctggatcagc
     1321 tgtttcgcct gatcgccgcc gagcagccgg cccgcctggt agttggcggt ggcggccacc
     1381 gcgggcacgc ggtcttcgcg cgccttcaga atcatctgca ccaaccccag cgtcttctgc
     1441 ttcaccgcgt cgggcacgtc cttcttactg aggatcgagc ggacgtaact gctcagcagg
     1501 ttcaccgcgt tgatcttgtc ggcgggcttg ccatcctgca gcgcggcgat ggcgcgctgc
     1561 ggcgccggcc cgtccggcgg ggcggcgctg cggttcatca ccttgctgaa cttcaccgca
     1621 tatccaccgg gcccggcgct cagctcgcct ttggggccgg tcagcggatt ggtgacgacc
     1681 gaaccataca cctcgaacgc gacggtggga tcgttctgca ggaactgaag cagcgggcct
     1741 tggtccaggc ggatgacctg cgccgtcgat tgccgcgagc gcagcaccat cggcccagcc
     1801 acacggtcca gcgcggtgcc gacgaacgat tgctcgacga tgcccttgac ctgcgcgttg
     1861 aaccacaggt cggggcgaat gacgccttcg gagccgatgg tcaggtcggc attgcccatg
     1921 ttgacgatcg acaggcgggc cagcagcggc tcatcgtagt cgcgcgcgac caccagtggg
     1981 tccacgtgca ccgcgtaaaa gctttgcggt tgatcgagca cgtgcatgaa gtccttggga
     2041 aattgctcgc cgatttgccg cagggcatcg gcttgtttac tggtgacgat cttcacgccc
     2101 aatggccgca ggccgtccca caggatgccg ccgatcaggc ccgacggata atcggagacc
     2161 agctttcgcc ctaaggtggc ggcgagttcc ttgttggact ccatgcgaat caggccgagc
     2221 gtcgccatcg gatcctgagc ggcgatggcg gagaacttca ccttcgcctc gtcgttcttg
     2281 ccctggatga ggaagagcca accttcgagc cgcgcaacgg ccgggtcgtc gtcggggcgg
     2341 atctgcttga gcacgtcgat gagcttcgat ccggcttcgg gatcctgcgc gaaatacgtt
     2401 tcgagcatcg ccagccggga gacggcttcg gtgaacgcct cttttagttc cggcggggca
     2461 tcctggttaa tcttggcgat cgtcaagcgc aagtccggca gcgtgtacgg gccttcggcg
     2521 gtgatcgggc gcgtcgtggg tttgtcgccg gtggtgccgc cgatctggtt caccagctcg
     2581 atcacgcgat tggacatcgc gttgcgggcc tgctgcagta ctttggtgaa gtcgtccttg
     2641 ttggccgccg agctgtggac gatggcgagc ttcaggaacc agatcgacac gttggccgga
     2701 tcggcggtga gaatgttgtc caccatctgg ttcgcggtgt tggcgtcacc ggcgatgaac
     2761 aacgcggcgg cctgtttggt ggcggcgctg atgctgggat gaccggtgcg cgcgctgatg
     2821 ttcagcgcga ggttgtacca gggcacggat tcggcgacga gggcggattc ggcgaactgg
     2881 tcggccagca tctcagtgta cgcggcttga cccgggttgg cgcggatcag ctccatcagt
     2941 gcgctggcgc gctcgaagtg cgaagccgtc ggcgacagca attgatatcg aagccgcagg
     3001 ccttcaccgc tgagcgggtt cgcccgcagc gcttcgccca ggacgttctt gccttgctct
     3061 tcctgcccgc ggtccaccag cagcttggcg cagacgacgg cggcatgtgc ccgcacgccg
     3121 tcgggcaccg tcggtgtgcc catgatggtc ttgaggtagg cgatcttcgc ctcggcggtg
     3181 tccatcttgt tcgcgtatag gtcgatcgtc tgggcctgcg cgaattcatc tttcggatcg
     3241 gccgcgcgca cgctgcgaag ggcgtcgatg gcgccctcgt catcgtgcag gcgaaggtcg
     3301 gcgtcagcca acagccggcc gacgcgcgcg ttcgtggggt cgagcttgtt agcggcttga
     3361 agaatggcgg cgacctgctg cagcatcggc tgccacagtt ccggggccgg caccttcgac
     3421 ggaatggccg cctgcgcgat gctggcgaat tgctcggcga gtacgcggtc gccttcgatc
     3481 tccgtcggat tggtcgtcgg cggggcggga ttcaatggcg gcgcgccggc ggcggcggcg
     3541 gtgtgggacg tcgtgggttc ggtggtcgct tcggtggtcg gtctcgtcgc cggttcggtg
     3601 gccgctgttg cggtgactcc ggcggctact cctgctgctg cggcgccggg cattgccgtg
     3661 gtcgtttcgg gcgatggtgt gacggctgcg acagccggct cgttcgccgg ttgggtcacc
     3721 ggcgcttcgg cggtcagctc cgtcgttggc gcggttgtcg cttccgctgt cggttccgtt
     3781 gtcaccggcg caggcgttgt cacttcgctc ggctcagtcg agcttggctc tttcggcgcc
     3841 ggcgacacgc tggccacggg cgtggtcttg gtggcgttgg tcgatggctt cggatggcaa
     3901 tcggccgcgg tgatcaccag cagcgggaac gtgatggcga ggagggtccg accggcggtg
     3961 gttgaattca gcgtgggcat ccgcattctg tgtttgcctt gcatgttgtc ttgcatggtg
     4021 gacgaccctc gcggccactg gcgtggcacg cgcgcgggcc gcgattggtt ctttatcgac
     4081 cggccaggct gagggcaatc gcgctgacca gggcgacggt gaagacggcg gcggcgagga
     4141 cgcgcagcac gatgtcgagc ccccgcgtct gataaattcg accgacgacg aacagcaaca
     4201 gcaccgcctg ggccagcgcc accgcggcga cggcggtggt gcgaacggcc cagagggcga
     4261 tgtccgggcg gtcggcgtcg aacaccaggt gcggggcctg ctgccaggcc cacagcgatg
     4321 cgaccatcaa tatcgccgcc agcgctgtgg cgctgagcat catcagtttt gcgatcatgg
     4381 caacctccgt gttgcgctga ttgtcttgcg actcgcttaa gcgagtcggc cggtcgtttc
     4441 ctgcgaccag agttccttca acaatggtcg ctccgcgacc agggcgttga catcgaccag
     4501 cagctccgcg cggcggcgga gccaggtgga ccaatcatcg cggcccgtct tccgcgccgc
     4561 cagcgccagc agcgattgtg cgcgggcggc ggtggtggac ggcatcaact ccgccaactg
     4621 taccttggcg ggcagattct gcaccatcgc gggccacgcg gcgacgcgcg attcgtaatc
     4681 cagctgcacc gccagcaggt tcatcgcctc ggccaacgtc aggcgctggt tgtacagccc
     4741 gaccagcgcc gcgtgcgcgc cgcgcaggtc gccgagttcc atcaacgaat cggccagcat
     4801 caaccggctg gagcgcgata atccctgcaa cgaaccgagc cgctggtgca gcaatgcttc
     4861 gctcagcaac gccgactcgc gccatcgtcg ctgcgcgtgg cggagcactg ccaggtgatg
     4921 aaggctgagc agcttcaccg tgcggaagat cgagaacgtc cgcagcacct gctcgatgcg
     4981 atgctcggct tcgtcgaatt gcccggaggc gatcagcagc ggcgaatccg ccgccagtga
     5041 tgagcctttg gcgctgcggt agctgaggat gagccagatc gcgccgatgg cggcgacggt
     5101 cagcgtcagc ggaaagcgcg agccgaccac gggcccggcg atgaggcaca gcagtgcggc
     5161 gccgacgagc agccccttca gcagcgaagc gaccgtcagg tcgcggcgaa gcgcgcggat
     5221 ggcggattca gcgctgatca tgcgagagcc gccccttcgc gatgccgcgc ccataagtcg
     5281 aatatcccat ttagcccggc gtgaacacac gccgggtttg cgtgaggctt caagaacctc
     5341 tggttgagaa aacgaaaccg cctcgacttc tcagcataac ccggcgtgtg ttcaccccgg
     5401 gctaaatggg gggcatcgcg ttcggttggg gcctttggcg cgacgatcac atcgcctccg
     5461 gcggcgccgg ggccggttgg tatttcgcgg tcagcttctg gacttcggca tatcggcgag
     5521 tcagctctga cacgggcgac agcagcgtcg cgcgctcgaa cgcggcccgc gcttcgtctt
     5581 cgcggccgag catgtcgtac gctcgcgcga ccagcgcgtg ggcgtccgcg acgcggtggc
     5641 ccagttgctg ctgcatcagc ggcagctttt cggcgaatga ctcgatcgcc tcggtgggat
     5701 ggccggtctt cacgtcgcgg taaatctcga ccagcgccaa gccgccggaa tgatcggaag
     5761 ccccgccgcg gcggagatcg gcgatggcct ggtgggcatc gacgaggtga tcttcatgca
     5821 gcatcgccat cgcccggccg agccgcaggc cgtacgcggt ggggggatcg accaggtcgt
     5881 tctcaagcag atgattctgc acggcgatcg cgtcttcgta acgatgatgt cgggccagca
     5941 ccgacgacag atacaccagc gcctgagccc gcgcccgtgg cgagcgcgcc ggcgtggaga
     6001 ggaacagctg aagggccatc cccgcctggt cccagcgtcg cagctgcacc aattcttcga
     6061 tcgcctcgac cgtctgttgc tgcgcgcgat gctggcggac ggtcaacatc gtcaccatcg
     6121 tcatcgcgcc cattaggccg aacaggacca gcagcgaaag cagttccacc gcctggtgca
     6181 tctgctgcga gcggctggac gccatcgtgc tgccgatgac gagcattgca aacaggccag
     6241 cgccgaacca gaaccagttg ccgcgcgggt gcggctggct taattccagc agcgtgggca
     6301 cgtcgagata acccggggac ggcggcggcg aagttggtga cgacgcgtca gccgcgtccg
     6361 tgacggtcat ggttggcgga ttctaggttc tggggacgta
//
LOCUS       DATIMH010001133         4792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5758798, whole genome shotgun sequence.
ACCESSION   DATIMH010001133 DATIMH010000000
VERSION     DATIMH010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5758798"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VLI90_19565"
     CDS             complement(<1..700)
                     /locus_tag="VLI90_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV16470.1"
                     /translation="MNMSITPVRRISGRRRLNRRNRRSAFTLIELLVVIRIIAVLISI
                     LLPALRRARESAQRVQCLSNMRQITGAIIAFANDHSGRMPSAATWTPLKIDPVTGDFK
                     TLQNGPWGSADIDPQRTFSPDWISWQRVKDEFSPTTVNTCAHLNITYSAVSRYLGGKL
                     IETFTTNDDNSANRALDAIYRCPSDDLYTRLSHADPSHGFYRYSYTMNLAYGSPPTGS
                     SFYAFPGYVKKPTGG"
     gene            complement(1079..4522)
                     /locus_tag="VLI90_19570"
     CDS             complement(1079..4522)
                     /locus_tag="VLI90_19570"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HSV16471.1"
                     /translation="MDFHDSGAGPLPGPYGGNQVNFPIAVSAGVVLHAPNGDYLSLPT
                     GTYVTVGFDDETAIDGPGNDIFVTEDTNAGEQASVQVSADGVNFTTIGTAFGGDTTGF
                     DLSDIGFTQPVRAVRVIGLDNNGSSPGFDLTDVQVAAGSMGAGLGPLVQAPTAHRPVL
                     IVPGLLGSLPTVKTLTDFLTTPGASPLTLSPDFVLRSYDPLLGSLTRLGYKQNVDLFV
                     ATYDWRLAIAPPDGVNDGVVHLNGKVTFNDQTWRYGVQYLDYWFRQAQAAWESANSGS
                     LAGFSVDIIADNFGGLLTRAFLQSDRFHDQYAGQVHDVVTLGTPYQGSIDAFLAIAPN
                     IGIDSLVRNVATEVEGDPLGLAGLKTFWDMAKSTGQVGSVLSLWSPSLLDLLPTYNVV
                     SPSAFTPDLSNLTNPLLPDLNADPSAFTAAVTDKVTVRAATGLDTALAATYLTGNNVF
                     ISKTTDGDGGVITSTTAVPGAVNPTPYTGIAHVDLPAAPQVILDVLQTDLGLTLPAGF
                     APTTTPLFGALAHFIWGWTNRVDFSFVDSLGRHFSSIDDEFTSEIPGAFFSGNGSPEY
                     FFIPLADAPGDGQLTLIASDPQNYRGGVIILSGNAIVHQTLSGNLTTNQSLPFELSSP
                     VLPTITAADAGIAEPLAGNRVLAVAVTLSSAPAVDVTMQYTTADGTATAGLDYTAVSG
                     TLTIPAGQTTGTILVPILGDLLVENDETFNVVLSLPTNAELNTAGGADRATVTIRNTV
                     FTAQPLVNGRATYTDAAGTSVNVRLIGPGDVQLLFANGSVVPAALLATGTTARSAVAI
                     LPTRGTTTFGDPAVGGISIAGPLAGLIGPNVNLLGGLTSSGAIGRIILRDVTGSAAFA
                     IGGGSQLIALGNVDNLDLTAAGPVQTIVATQWLADDHQPHLITAPSIGTLFVRGALAD
                     GISLSSTGNALGLARIGGSIDAGAWSIAGAAGTILARSSSPAWTANFGGALGVVSFTG
                     NLAGTLSARAVRVLRTGGDLAAATLRFSSASTDVNTPAVGTLFIAGAINHSTILAAGS
                     VRTVVAGSMTSSTLFSGIAQTVNSLPSTTDDFVARTHMSTFLIRPGGVFSDSAVAAAH
                     ILNVVLQNVQTQNNGNVFGFSTESIKTFSAASPRIHWNDRFSESLLQSLLNDLRVRIL
                     S"
     gene            4511..>4792
                     /locus_tag="VLI90_19575"
     CDS             4511..>4792
                     /locus_tag="VLI90_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16472.1"
                     /translation="MEVHDFVRVSIAGQQRALLERLDFDRRRRSLTATAVAQAAWHFL
                     PSVRRESPTRNGPTIVLTAADRRYKKIPSSAAQRRSSRLPNVPLGPFLRF"
BASE COUNT          865 a         1468 c         1606 g          853 t
ORIGIN      
        1 tcccgccggt gggttttttc acgtaaccgg gaaacgcgta gaagctcgat ccggtcggcg
       61 ggctgccgta ggcgaggttc atcgtgtagc tatatcggta aaacccgtgc gatggatcgg
      121 cgtggctcag gcgcgtgtac aggtcgtccg acggacaacg atagatcgca tccagcgcgc
      181 gattggccga gttgtcgtcg ttggtggtga acgtttcgat cagcttcccg ccaaggtacc
      241 gcgacaccgc tgaataggtg atgttgagat gggcgcaggt gttcacggtc gtcggcgaaa
      301 actcgtcctt gacgcgctgc cagctgatcc agtcgggcga aaacgtccgc tgcggatcga
      361 tatcggctga accccacggt ccattttgaa gggttttgaa gtcacccgtg acggggtcga
      421 tcttcagcgg cgtccatgtg gcggccgagg gcatccgccc ggaatgatcg ttggcgaagg
      481 cgatgatcgc gccggtgatc tgccgcatgt tgctcaggca ctgcacgcgc tgcgccgatt
      541 cccgcgcccg ccgcagcgcc ggcagcagaa tgctgatcag tactgcaata atgcgaatta
      601 caaccaggag ctcgatcagc gtgaacgccg atcgccggtt tctccggttc agcctgcggc
      661 gacccgaaat ccggcgcacg ggtgtaatcg acatgttcat ggcgtctcct cgcttacctg
      721 catcgcagag ccttcgacgt gtgggccggc tcctggggga cgaaaggatg gcaggaatct
      781 actgcgcgcg tgccgaggaa cgcattacag ctttacgaaa tgttattgca atgcagcgca
      841 gggcgcggat gcgcgtctgc ccacgccggg gcttctgagc gtaccgtacg ggccaagaca
      901 cggtttcaaa acttcggcaa cgcctcaccg tgacacgggc ctccggcctg tgcgtcgtgc
      961 gccaaagtgt gaggaagcga cggcatttct actaccaacg cattacgcac gggccggagg
     1021 ctgccggagg cccgtgtcac gatgaaggta atgcaaaccg ctcgtacacc ggattctctt
     1081 atgacaatat ccgcacgcgc aggtcgttga ggagcgattg cagcagcgat tcgctgaacc
     1141 ggtcgttcca atggatccgt gggctggcgg cgctgaacgt cttgatcgat tcggtggaga
     1201 agccgaagac gttgccgttg ttttgggttt gaacgttctg caacacgaca ttcaggatgt
     1261 gcgcggcggc gacggcgctg tcgctaaaga cgccgccggg gcggatcagg aatgtggaca
     1321 tatgggtgcg ggcgacgaag tcgtcggtag tgctgggcag cgagttgacg gtttgcgcga
     1381 tgccgctgaa gagcgtcgag ctggtcatgc tgccggcgac gacggtgcgc acgctaccgg
     1441 cggccaagat ggtggaatgg ttgatcgcgc cggcgatgaa cagcgtgccg acggcgggtg
     1501 tgttcacgtc ggtcgatgcg ctgctgaagc gcagcgtggc ggcggcgagg tcgccgccgg
     1561 tgcgcagcac gcgcaccgcg cgggcactga gcgtgccggc gagattgccg gtgaagctga
     1621 cgacgccgag cgcgccaccg aagttagcgg tccaggcggg ggatgacgag cgcgccagga
     1681 tcgtcccggc cgcgccggcg atgctccagg cgccggcatc gatcgagcca ccgatgcgcg
     1741 cgaggcccag cgcattgccg gtgctgctga gcgagatgcc gtcggccagc gcgccgcgga
     1801 cgaacagcgt gccgatcgac ggcgccgtga tcaggtgcgg ttgatggtca tccgccagcc
     1861 attgcgtggc gacaatcgtc tgcaccggcc cggcggcggt gaggtcgagg ttgtccacgt
     1921 tgcccagcgc gattaattgg ctgccgccgc cgatggcgaa ggcggccgat ccggtcacgt
     1981 cgcgcaggat gatacggccg atcgcgcccg aactggtcag cccacccagc aggttcacgt
     2041 tgggaccaat cagccccgcc agcgggccgg cgatgctgat cccaccgacg gccgggtcac
     2101 cgaaggtggt cgtgccgcgc gtcgggagga tcgcgacggc cgaacgggcg gtcgtgccgg
     2161 tggcgagtag cgctgccgga acgacgctgc cgttggcgaa cagcaactgc acatcgccgg
     2221 gaccgatcag gcgaacgttc acagatgtcc ccgcggcgtc ggtgtaggtg gcccggccgt
     2281 tgaccaatgg ctgggcggta aacaccgtgt tgcgaatggt cacggtcgcg cgatccgcgc
     2341 cgccggcggt gttcagctcc gcgttggtgg gcaatgaaag cacgacgttg aacgtctcat
     2401 cgttctccac gagcaggtcg ccgaggatcg gcaccaggat cgtaccggtc gtctgccccg
     2461 cgggaatcgt gagcgtgccg ctgacggcgg tgtagtccaa gcctgccgtg gccgtgccgt
     2521 cggccgttgt atattgcatc gtcacgtcga ccgccggcgc gctgctcagc gtcaccgcga
     2581 ccgccagcac gcgattgccc gccagcggtt cggcgatgcc ggcgtcggca gccgtaattg
     2641 tcggcagcac cggtgacgac agctcgaacg gcaacgattg gttcgtcgtg aggttgccgg
     2701 acagcgtttg atgcacgatc gcgttgcccg acaggataat gaccccgccg cggtagttct
     2761 gcggatcgct ggcgatcagc gtcagctgcc catcgcccgg ggcgtcggcc agcgggatga
     2821 agaaatattc cggcgatcca ttgccgctga aaaaggcgcc ggggatttcg ctggtgaact
     2881 catcgtcgat gctggagaaa tgccgcccga gcgaatccac gaacgagaaa tcgacgcggt
     2941 tggtccagcc ccagatgaaa tgcgcgagcg cgccaaacag cggcgtggtc gtcggggcaa
     3001 atcccgccgg cagcgtcagg ccaaggtcgg tctgcagcac gtcgaggatc acctgcggcg
     3061 ccgccggcag atcgacgtgc gcgatgccgg tgtacggcgt cgggttcacc gcgccgggca
     3121 ctgccgtagt tgacgtgatt actccgccgt cgccatcggt ggttttggag atgaagacgt
     3181 tgttgccggt caggtaggtc gcagccagcg cggtatcgag tccggtggcg gcgcgaaccg
     3241 tcaccttgtc cgtcacggcg gcggtgaagg cgctggggtc ggcgttcaag tccggcagca
     3301 gcgggttggt caggttcgac aaatccggcg tgaaggcgct cgggctgacg acgttgtacg
     3361 tcggcagcag atccagcagg ctgggcgacc acaagctcag cacgctgccg acttggccgg
     3421 tggacttggc catgtcccag aacgttttca gtccggccag accgagcgga tcgccttcga
     3481 cttcggtggc gacgttccgc acgagtgagt cgatgccgat gttcggcgcg atcgccaaaa
     3541 atgcgtcgat cgatccttga tacggcgtgc cgagcgtcac cacatcgtgc acttgcccgg
     3601 cgtattgatc gtgaaagcga tcgctctgca aaaacgcgcg ggtgagcaat ccaccaaagt
     3661 tgtcggcgat gatgtcaacg gagaagccgg ccagcgagcc gctgttggcc gactcccacg
     3721 ccgcctgcgc ctggcggaac cagtagtcca gatattgaac gccgtaccgc caggtctgat
     3781 cgttgaacgt gaccttgccg ttcaggtgca cgacgccgtc attcacaccg tccggcggcg
     3841 cgatcgccag ccgccagtca tatgttgcga cgaacagatc gacgttctgc ttgtagccca
     3901 gccgcgtgag cgacccgagc agcggatcgt aactccgcag cacgaaatcc ggcgacaacg
     3961 tcagcggcga cgcgcccggc gtcgtgagaa aatcggtcag cgtttttacc gtcggcaatg
     4021 accccagcaa ccccggcacg atcagcaccg gccgatgcgc cgtcggcgct tgcaccagcg
     4081 gccccagtcc agcgcccatg ctgcccgcgg caacctgcac gtcggtcaga tcaaacccag
     4141 gcgatgaacc gttgttgtcc agcccgatga cgcgcaccgc ccgcacgggc tgcgtgaagc
     4201 cgatgtcgga tagatcgaag ccggtcgtat ccccaccaaa cgcggtgccg atggtggtga
     4261 agttcacgcc atcagcggaa acctgcacgc tcgcctgctc gccggcgttg gtatcttcgg
     4321 taacgaagat gtcgttaccg ggaccgtcga tggccgtctc atcatcgaag ccaacggtga
     4381 cgtaggtgcc cgtgggcagc gaaagataat cgccattggg cgcatgcagc acgacgcctg
     4441 cgctaaccgc gatcggaaag ttgacttgat ttccgccata cggtcccggc aacggcccgg
     4501 caccgctgtc gtggaagtcc acgacttcgt ccgcgtatcc atcgccggcc agcagcgtgc
     4561 gctgctcgag cggctcgatt ttgaccgcag acggagatcg ctgaccgcga cggcggtggc
     4621 tcaagcggct tggcattttc ttccttctgt ccgacgcgaa tcacccacgc gcaacggtcc
     4681 aaccattgtc ctaacagcgg cagaccgtcg ttacaagaaa attcccagta gcgcggcaca
     4741 gcgtcgctct agccgcttgc cgaatgttcc gttggggccg tttttgcgtt tt
//
LOCUS       DATIMH010001134         3930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_560689, whole genome shotgun sequence.
ACCESSION   DATIMH010001134 DATIMH010000000
VERSION     DATIMH010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560689"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..1082)
                     /gene="rnc"
                     /locus_tag="VLI90_19580"
     CDS             complement(339..1082)
                     /gene="rnc"
                     /locus_tag="VLI90_19580"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HSV16473.1"
                     /translation="MNTELRERAEKILQYTFKDPDLLKESLTHASIADNRLSSNERME
                     FLGDAVLDLIVCEALYKRFPEYLEGDLTKIKSAVVSRRTCAEVSNETGLTDLLIIGKG
                     ISSREAMPSSLAAAVYESIVAAIYLDGGFEAIKEYVLRTMTPKIEVISANSHQQNYKA
                     VLQQHAQKILGATPIYELMDEKGPDHSKCFEVCVSVDGRRFTSAWGPNKKMAEQKAAL
                     LALEELGVLAPEEVDEAIEEIATETETPP"
     gene            1487..3595
                     /locus_tag="VLI90_19585"
     CDS             1487..3595
                     /locus_tag="VLI90_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16474.1"
                     /translation="MDVRSSSQARSRSLALGISLLFCLRLAAARADIPDNAGDTLFDP
                     ARHMRVAEVRPGMTGYGLSVFQGTKIERFDVEVVSILKNFNPKYDVVLIRCHGANLEH
                     SGPIAGMSGSPIYLSDDSGRSRMIGAFAYGWPLQKDPVAGVQPIEYMLKLPLAKGSPA
                     TQPGNDGGAPMASPLKEGSAVRQNATWSLSDSVLLPGMTTPPPNYPLAAWGTMTPSPT
                     LLSGNGEATRMQPLATPLMMAGMPTSVMREFEPLFRAYGLVPLQAGGGADASVDPAIE
                     PGSVLAVPLVSGDVDVSAVGTCTEVVGDHIVAFGHSFNNEGPIALPMGSGSIQGVIAN
                     LQTSFKLGALAKIKGTLTEDQTVGIAGKLGAAPPTVPVEIRVRYADGSEDQTYHFGIT
                     LHPKLTPMLSVAAVGAAISGARELPQYHTVDYDLTLDFSNGKQIHVINTTVNSGGPEL
                     FFDVGTPLIAAADNPFERVFVKRMTGSITVTPEARQGEILYANVPRSRYRPGDTVTAY
                     VTYRPFRATERVLPVTLELPKDLPDGTYQLTISDWTRFLSDEQTAEPFKFHAESIDQV
                     FDVLRDMTAIRHNALYIRLLRQPDGIAIGHTAMPKLPSSRRQVMIGAGRSDTTQFVSS
                     AVKVIASDRVMSGSAEFEITIDKNANTEAPTPAARPPHRDVRPQPKPAAVPEGAPKPP
                     AAGKQEPPAAGGPTGASPPE"
BASE COUNT          751 a         1326 c         1158 g          695 t
ORIGIN      
        1 cgaggctcca aaacagaaac tcagcgttga tcaaaaccgc gaggcctcgc gaacaggaag
       61 tggaaatacc atcaatcact cgatggcgca aatgtggggc gcgccgttat ttacttgaag
      121 ccaaatgatt ctgccctgca aggcggcgat gcgcgaccag acgcatagtc cgggaatacc
      181 cccatttagc ccggcgtgaa cacacccggg tttcgttgag aaggcgagac cgttcgattt
      241 ctcaatcaaa ggatctcgac gccccacaca aacccggcgt gtgttcacgc cgggctaaat
      301 ggggtggtct gcgttcgatc gccggcgcgc aactccgatc acggcggggt ctcggtctcg
      361 gtcgcgatct cctcgatcgc ctcgtccact tcttccggcg ccagcacgcc cagctcttcc
      421 agcgccaata gtgccgcctt ttgctcggcc atctttttgt tcgggcccca cgcgctggtg
      481 aaccgccggc catcgacgga gacacagacc tcaaagcact tgctgtgatc cggccccttt
      541 tcgtccatca gctcgtagat cggcgtggcg cccaagatct tctgcgcgtg ctgctgcagc
      601 accgccttgt aattctgctg gtgcgaatta gcgctaatga cttcgatctt cggcgtcatc
      661 gtgcgcagca cgtattcctt gatcgcctcg aacccgccgt ccagataaat ggcggccacg
      721 atcgactcat acaccgcggc ggcgagcgag ctgggcatcg cctcgcgcga gctgatgccc
      781 ttgccgatga tcagcaggtc cgtcaaaccc gtctcgttcg acacttccgc gcacgttctg
      841 cgcgagacga cggccgactt gatcttggtc aggtcgcctt cgagatattc ggggaagcgc
      901 ttgtagagcg cctcgcagac gatcaggtcc agcaccgcgt cgccgaggaa ctccatgcgc
      961 tcgttgctcg acaggcggtt gtcggcaatg gaggcgtggg tcagcgactc cttcaacagg
     1021 tcgggatcct tgaaggtgta ctgaaggatc ttctccgcac gttcgcgcag ttcggtgttc
     1081 acgctgggct gccttggttg tgtctgttgc tggaccgact catcgacaag agtctggact
     1141 cgggcgcgcg acggagagtg tggcaggcat ttacaagcag cgctacggtc tcatggttga
     1201 ccggcaggaa cgcgaatttc ggcgaccggc cagaaccggc ggatcggcga aactggtatt
     1261 ccccgcgacg cgcatcgctt cggcgtcaca cgctcacaac gaatcggcgc accgaataat
     1321 caacggcgaa attatctgtt ttcttcaaag gtgtcaacgg aattaccgtt accccactta
     1381 tcggcaactc gcccaccttg ccatgtttgc acgcgcgttt taccatgacg atttggcctc
     1441 tgccgggctc gcgcgcgtat gtgttgtgtc ccctcgaggt ggttgcatgg atgtccgcag
     1501 cagttcccag gcccgcagcc gctcgctagc gctcggcatt tcgctcctgt tctgccttcg
     1561 gctggccgcc gcgcgcgcag acatacccga caacgccggc gatacgctgt tcgacccggc
     1621 ccgccacatg cgcgtcgctg aagtccggcc gggcatgacg gggtatgggc tgtccgtctt
     1681 ccagggcacc aagatcgagc gcttcgacgt tgaagtcgtc tcgatcctca aaaacttcaa
     1741 tccgaaatac gacgtcgtgc tgatccgctg ccacggggcg aacctggaac acagcggccc
     1801 gatcgccggc atgagtggct cgccgatcta cctgtccgac gacagcggcc gatcgcgcat
     1861 gatcggcgcg ttcgcctacg gttggccgct gcagaaggat cccgtcgccg gcgttcaacc
     1921 gatcgaatac atgctcaagc tgccgctggc caagggctcg cccgcgaccc aacccggcaa
     1981 cgacggcggc gcgccgatgg cgtcaccgct gaaggaaggc tccgccgttc gacaaaacgc
     2041 gacgtggtct ttgagcgact ccgtcctgct gccgggaatg accaccccgc cgccgaatta
     2101 cccgctggcg gcgtggggca cgatgacgcc gagcccgacg ctgctcagcg gcaacggcga
     2161 ggcaacgcgc atgcaaccgc tggcgacgcc gctcatgatg gccgggatgc caacgtcggt
     2221 gatgcgagaa tttgagccgc tgttccgcgc ctatggcctc gtcccgctgc aagccggcgg
     2281 tggcgcggat gcgtcggtcg atccggccat cgaacctggt tcggtgctgg ccgtcccgct
     2341 ggtttccggc gatgtggacg tgagcgccgt cggcacctgc accgaagtcg tcggcgatca
     2401 catcgtcgcc ttcgggcact cgttcaacaa tgaaggccca atcgcgctgc cgatgggcag
     2461 cggttcgatc caaggcgtca tagccaacct gcagaccagc ttcaagctcg gcgcgctggc
     2521 gaagatcaaa ggcacgctga ccgaggacca gacggtcggc atcgccggca agctcggcgc
     2581 cgccccgccg accgtgcccg tcgagatccg cgtgcgctat gccgacggca gcgaggacca
     2641 gacctatcac ttcggcatca cgctgcaccc caagctcacg ccgatgctca gcgtcgccgc
     2701 cgtcggcgcc gccatcagcg gggcgcgcga gctgccgcaa tatcacacgg tcgattacga
     2761 cctgacgctc gacttcagca acggcaagca gatccacgtc atcaacacca ccgtgaactc
     2821 cggcgggccc gaactgttct tcgacgtcgg cacacccctc atcgccgcgg ccgacaaccc
     2881 gttcgagcgc gtcttcgtca agcgcatgac cggttcgatc accgtgacgc ccgaagcccg
     2941 gcaaggcgag atcctctacg ccaacgtccc gcgctcgcgc tatcgtccgg gtgacaccgt
     3001 caccgcatat gtcacctatc gccccttccg cgccaccgag cgcgtcctgc cggtgacgct
     3061 ggagctgccc aaggacctgc cggatgggac atatcagctg acgatttccg attggacgcg
     3121 cttcctttcg gacgagcaga cggccgagcc gttcaagttt cacgccgagt caatcgacca
     3181 ggtcttcgac gtgctgcgcg acatgacggc catccgccac aacgcgctgt acatccgcct
     3241 gctgcggcaa ccggacggca ttgcgatcgg tcacaccgcg atgccgaagc tgccgtcatc
     3301 gcgaaggcag gtgatgatcg gcgccggcag aagcgacacg acgcaattcg tcagctccgc
     3361 ggtaaaagtg atcgccagcg accgcgtaat gagcgggtca gcggagttcg agatcacgat
     3421 cgacaagaac gcgaacaccg aagcgccgac gccggccgcg cgtccgccgc accgcgacgt
     3481 gcgcccgcaa cccaaacccg ccgccgtacc cgaaggcgcc cccaaacccc ccgcggcggg
     3541 gaagcaagaa ccaccggctg ctggtggacc gacgggcgcg tcgccaccgg aatgattcga
     3601 tcgattggat tttaaatcgc gagcgaaatg actgtagaag ccgtccatcc gcagaaccga
     3661 ggcagaggag cgacgaacgc gatagtagcc gcaggcgaca gccagcgggg gctcgcgacg
     3721 aaagagcgct tcgacttcgc aagcgcccgc tggctgtcgc ctgcggctat caaggggtct
     3781 caaatcgaac ctgcctaacg cgcgactgca tttaatatca acaccatgaa acgcctaatt
     3841 ccccttatcc tcctcattac cgccctgccc gccatggcgg tgacgacttc acactggatg
     3901 cacaccaacg aggccgactt caagcccggc
//
LOCUS       DATIMH010001135         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1138257, whole genome shotgun sequence.
ACCESSION   DATIMH010001135 DATIMH010000000
VERSION     DATIMH010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138257"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VLI90_19590"
     CDS             complement(<1..275)
                     /locus_tag="VLI90_19590"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSV16475.1"
                     /translation="MIKSSEPVFAVADVRATITYYRNVLGFESEWLWGDPPTFGGVCW
                     GRIQVMFCLDPTLQSKVQGHQQFFRLDNVQELYDRHEAAGATIVCAI"
     gene            complement(281..1108)
                     /locus_tag="VLI90_19595"
     CDS             complement(281..1108)
                     /locus_tag="VLI90_19595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV16476.1"
                     /translation="MIWILILATWEAAYRFIGWKAWVFPAPSHIFDSLFSLLDIHTAF
                     GEPLHRGWPWPAGAPGGISTGVIRSPLVEANIVSAVRLIIGFFLSILLGGTLGVLMWR
                     FKTLDEFLGPLFLGLQTLPSVCWVPLAIIAFGLTEEAVQFVLVMGSFFAIAISLRDGL
                     RTIPPLYQRAGMMMGAHGWRLYRYVLLPASLPALASGLRQGFSFAWRSLMGAEFVFII
                     ERHGLGYLLQAGRDFNDVAQVMAIMIVMVVLGMIADRLAFAKLEQRVYRRFGLAAAR"
     gene            complement(1065..1994)
                     /locus_tag="VLI90_19600"
     CDS             complement(1065..1994)
                     /locus_tag="VLI90_19600"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV16477.1"
                     /translation="MTLAEQSPKTISIALPKARPGRGHLALSNIDKNFDGSSVPALRG
                     VSITTEPGEFVVVVGPSGCGKSTLLNVAAGMYTPDNGTVTLDGETVDGPGPERAMVFQ
                     DHGLFPWLTALQNVEFGLKMAGISRKDRHERAAAALKMVHLSNSGHKLVHELSGGMRQ
                     RVAIARALVMDPAVLLMDEPFAALDAQTRTLLHEQLQDLWIRTRKTILFVTHSVGEAV
                     RLADRIIVLHAHPGRVRRQVAVPLPHPRNFDSKQITELVHLVRTEIEDEVNRVNAQLS
                     GGTTTNGDLARDFWKPQASDDLDPHPGDVGGGI"
     gene            complement(2130..>2554)
                     /locus_tag="VLI90_19605"
     CDS             complement(2130..>2554)
                     /locus_tag="VLI90_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16478.1"
                     /translation="LLIATTGAKMIGEEIDLWPNKTFDLTVIVTTPEFLAAHPDVVQK
                     VLSVNHAWTTRLNAEPAKYVPQLEQALAKLTGKSLPPGVTSDALAHVKFNEEPAQETF
                     EANAQWAYDLGLAKEKPNLANLVDLSVLHKVPTATTAP"
BASE COUNT          480 a          832 c          779 g          463 t
ORIGIN      
        1 tcgatggcgc aaacgatggt cgcccccgcg gcttcgtggc ggtcgtacag ctcctgcacg
       61 ttgtccagtc ggaagaattg ctggtgacct tgcaccttcg attgaagcgt tggatccagg
      121 cagaacatca cctgaattcg cccccagcac acgccgccga aggtcggtgg atcgccccag
      181 agccactccg attcaaagcc cagcacgttg cggtagtacg tgatcgtcgc gcgaacatcg
      241 gcgacggcga agaccggctc ggatgatttg atcatctgca ttacctcgcc gcggcaaggc
      301 cgaaccggcg gtagacgcgc tgctccagct tcgcgaacgc caggcgatcg gcgatcatcc
      361 ccagcacgac catcacgatc atgatcgcca tcacctgcgc gacatcgttg aaatcccggc
      421 ccgcttgcag cagatagccc aggccgtggc gttcgatgat gaagacgaat tccgcgccca
      481 tcaacgatcg ccaagcgaag gagaagcctt ggcgaagtcc gctggccagt gcgggcaacg
      541 acgcgggcaa tagaacatag cgatacaagc gccagccgtg cgcgcccatc atcatgccgg
      601 cgcgttggta cagtgggggg atcgtgcgca gcccgtcgcg cagcgagatg gcgatcgcga
      661 agaagctgcc catcaccagc acgaactgca cggcttcttc ggtcaggccg aatgcaatga
      721 tcgccagcgg cacccagcag acgctgggca atgtctgcag gccaaggaac agcggcccga
      781 ggaattcgtc gagcgttttg aatcgccaca tcagcacacc gagcgtcccg cccagcagga
      841 tgctcaggaa gaagccgatg atcaggcgaa ccgcgctgac gatattcgct tcgacgaggg
      901 gactgcgaat gacgccggtg ctgatgccgc cgggcgcgcc ggccggccac ggccaaccgc
      961 ggtgcagcgg ttcaccgaac gcggtgtgga tgtcgagcag ggaaaacagc gagtcgaaga
     1021 tgtggctggg cgcggggaag acccacgcct tccaaccgat gaacctatat gccgcctccc
     1081 acgtcgccag gatgaggatc cagatcatca gacgcttgag gcttccagaa atccctggcc
     1141 agatccccgt ttgtcgtggt gccgcctgat agctgagcgt tgacgcggtt gacttcatcc
     1201 tcgatttccg ttcggaccag gtgaaccagc tcggtgatct gcttgctgtc gaagtttcgc
     1261 ggatgcggca gcggcaccgc gacctgccgg cgcacgcggc ccgggtgcgc gtgcagcacg
     1321 ataatccgat cggccaggcg aacggcttca ccgaccgagt gcgtgacgaa caggatcgtc
     1381 ttgcgcgtgc gaatccacaa gtcctgcaac tgctcgtgca gcagcgttcg cgtttgcgca
     1441 tcgagcgcgg cgaacggttc gtccatcagc aaaactgccg ggtccatcac cagcgcgcgg
     1501 gcgatggcga cgcgctgccg catgccgccc gataattcgt gcacgagctt gtgaccgctg
     1561 ttggacaggt gcaccatctt cagcgctgcc gctgcccgct cgtgacggtc cttgcgcgag
     1621 attcccgcca tcttcaggcc gaattcgacg ttttgcagcg ccgtcagcca ggggaagagc
     1681 ccgtgatcct gaaacaccat cgcccgttcg ggccccgggc catcgacggt ttcaccatcc
     1741 agcgtgacag tgccgttgtc cggcgtatac atccccgcgg cgacgttcag cagcgtgctc
     1801 ttaccgcacc cgctgggacc gacgacgacg acgaactcgc ccggctcggt cgtaatgctg
     1861 acgccgcgaa gtgcggggac ggaggagccg tcgaagttct tgtcgatgtt gctgagcgcg
     1921 agatgcccgc ggcctggacg cgctttgggc aacgcgatcg agatcgtctt cggcgattgt
     1981 tctgcgagcg tcatacgcac ccgttgtcgg tcgaacgatt ctttcaaagc cgcaggcggc
     2041 agccagcggg ggggctttcg agcaccagca tgtttgattc accaacgcag ccgctggctg
     2101 tcgcctgcgg ctactaaatg tcatcgcgat tacggcgccg tcgtcgcggt cggcacctta
     2161 tgcagcacgc tcaagtccac gaggttcgcc aagttcggct tttccttcgc caagcccagg
     2221 tcgtatgccc actgcgcgtt cgcttcgaac gtctcctgcg ccggctcttc gttgaacttc
     2281 acatgcgcca gcgcatcgct ggtcacgcca ggcggcagcg acttaccggt cagcttcgcc
     2341 agcgcctgct ccagctgcgg aacatatttc gccggctcgg cgttcagccg cgtcgtccac
     2401 gcgtggttca ccgacagcac cttctgaacc acgtccggat gcgctgccaa aaattccggc
     2461 gtcgtgacga tcaccgtcag gtcgaacgtc ttattcggcc acaggtctat ttcctcgccg
     2521 atcatcttcg cgcccgtggt ggcgatcagc agcg
//
LOCUS       DATIMH010001136         4494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1138314, whole genome shotgun sequence.
ACCESSION   DATIMH010001136 DATIMH010000000
VERSION     DATIMH010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138314"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1080)
                     /locus_tag="VLI90_19610"
     CDS             complement(22..1080)
                     /locus_tag="VLI90_19610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HSV16479.1"
                     /translation="MSNLQDILKTAIDNKASDVHVMVGAPPLFRLHTQMTPTDFPMVT
                     SESALRMVKEMLNEKRMEAFMEKRDADFSYEIPGLARFRVNAHFQRTSVAMAFRVITD
                     KVRALDALNLPEVITRLTYLPRGLVLVTGDTGSGKSTTLAAMIDAMNRRYHKHIITLE
                     DPIEYLLHSNKCAIEQREVGSDVPSFSSGLKHVLRQDPDIILVGEMRDLETTSAAITA
                     AETGHFVLSTLHTNNAAQTIERIIDIYPGEQQNQIRSMLSNTLQAVVCQTLFKRIDQP
                     GMVPGVEILICTPAVRNLIRENRIYEIHNVIETSRALGMQTMDTSIKQLYFNGKISKE
                     DAVSMAHHPEKLERQLVA"
     gene            complement(1150..3003)
                     /locus_tag="VLI90_19615"
     CDS             complement(1150..3003)
                     /locus_tag="VLI90_19615"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSV16480.1"
                     /translation="MAKSKVENWLEEMERESAATELAELPPVALVPSSEEALSVLWSP
                     QPSDSGKRKTVEEILAAQGKLDADKLLQARTVQANSRGKKVSQILHEMAAVGEVDIQR
                     AVAEMMGLEFEAIDPKKLDRRAFDYLPSDFMKARGCCGVRLDEGRLILGMVDPADIFL
                     LEEVKRRVTVKQVKVVVVCLSHIVAAIEAGNSTSSEGEKFDEIIKDMGEEELEIVAEE
                     KEELTDLAKASGESPVIRLVNFLIFDAIKQGASDIHIEPHEKRLVVRFRIDGVLFEAM
                     NPPAAMQAAVSSRLKIMANMDISERRLPQDGRIRTMVHGRHVDLRVSTVPTQYGEKTV
                     IRILDNRSITLGLEQLGFSEDMLVILRKQIDRPNGIILVTGPTGSGKTTTLYSCLRCM
                     DGAKMNISTVEDPVEYHLGFANQIQVMDRIGMTFAAALRALLRQDPDVIMLGEIRDTE
                     TAHIAVQAAMTGHMVLSTLHTNDAPSSVTRLINIGVEPYLISAAVNAVLAQRLVRRIC
                     GECKEPWKDMDADMREFLETRGFDPNNIFKGRGCDKCRNTGYKGRVGIYELLVLDDVA
                     RDLVVRNPNVTELRRICKERGMVSLREDGLHKVAAGSTTIEEVMSATENTM"
     gene            complement(3064..3777)
                     /locus_tag="VLI90_19620"
     CDS             complement(3064..3777)
                     /locus_tag="VLI90_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16481.1"
                     /translation="MSQQWKESTTGGDDDVQMPLGVGGDITPPEEYSESKGPRFNSST
                     LTLVAAFATAMGVLYLLGLQNKPRTASAEDDKVRQKIDERIQELVRSKTGQDGAGKRG
                     TAWLIEMLNRYFEASNKVVDLSRNPFEHEVTATVAEPPPGPREGPVLPPAASKELLDA
                     TMAYQGFRLQMVMTGSNPAALVSDSGTDQMVKVGSHLKMWEVLSIDPGRLVLRFKDVN
                     GKPTDVELKPNAVPLSNKH"
     gene            complement(3774..4334)
                     /gene="pilO"
                     /locus_tag="VLI90_19625"
     CDS             complement(3774..4334)
                     /gene="pilO"
                     /locus_tag="VLI90_19625"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HSV16482.1"
                     /translation="MQFGLRTVLYVVLVLGMLVMSYPLLFKPLNEQRAQAVADTKLKQ
                     QKLADLASAMRATQNMPEEITQLKKAIEFLTSKLPAQTEMDKVLDEVWKAAKENKLTV
                     KSVRNGKPIEGPNYNEQPIRMVVEGPFHPAFYKFLSQVEQMPRLTKIKEMKIEPDHDE
                     KKNGVIVADFELTIYYESNQNVAVAQ"
BASE COUNT          752 a         1634 c         1119 g          989 t
ORIGIN      
        1 gatccgttcg cgccgccggc ctcacgccac cagctgccgc tccagcttct ccggatggtg
       61 tgccatcgac accgcgtctt ccttgctgat tttcccgttg aagtacaact gcttgatcga
      121 tgtgtccatc gtctgcatcc ccaatgcccg cgacgtctcg attacgttgt gaatttcgta
      181 aatccggttc tcgcggatca aattccgcac cgccggcgtg cagatcagaa tctccacccc
      241 cggcaccatc cccggctggt cgatccgctt gaacaacgtc tgacacacca ccgcctgcaa
      301 cgtgttcgac agcatcgacc ggatctggtt ctgctgctcg ccgggataaa tatcgatgat
      361 acgttcaatc gtctgggccg cgttgtttgt gtgcagcgtc gagagcacga agtggcccgt
      421 ttccgccgcc gtgatcgccg cggaggtcgt ctccaggtcg cgcatttcac cgaccaggat
      481 gatgtccggg tcttgacgca gcacgtgctt cagccccgac gaaaagctcg gcacgtcaga
      541 gcccacctcg cgctgctcaa tggcacactt gttcgagtgc agcaggtatt cgatcggatc
      601 ttccagcgtg atgatgtgct tgtggtaccg ccggttcatc gcgtcaatca tcgccgccag
      661 cgtcgtcgac ttgcccgaac ccgtgtcccc cgtcaccagc accaacccgc gcggcaggta
      721 cgtcagccgc gtaataacct ccggcaaatt cagggcgtcc agcgcccgca ccttgtccgt
      781 gatcacacgg aaggccatcg ccaccgacgt gcgctgaaag tgggcgttga cgcggaaccg
      841 cgccaggccc gggatttcat acgaaaagtc ggcgtcgcgc ttttccatga atgcctccat
      901 gcgcttctcg ttgagcatct ccttcaccat ccgcagggcg ctctcgctgg tcaccatcgg
      961 aaaatccgtc ggcgtcatct gcgtgtgcaa ccggaacagc ggcggcgcgc ccaccatcac
     1021 gtggacgtcc gacgctttgt tgtcgatggc cgttttcaaa atatcctgca agttcgacat
     1081 tgacccatcc ttatctaatc aaccagcctc acgccgcaat ccgtcatact tcttacttct
     1141 tgcttcttcc tacatcgtgt tttccgtggc cgacatcact tcctcaatcg tcgtggagcc
     1201 cgccgccacc ttgtgcagcc cgtcctcgcg caagctgacc atcccgcgct ccttgcagat
     1261 ccgccgcagt tccgtcacgt tcgggttgcg cacgaccagg tcacgcgcca cgtcgtcgag
     1321 caccagaagt tcataaatgc ccacgcggcc cttgtagccc gtgttccggc acttgtcgca
     1381 gccgcggccc ttgaaaatgt tgttcgggtc gaacccccgc gtctccagga actcgcgcat
     1441 gtccgcgtcc atgtccttcc acggctcctt gcactcgcca caaatccgcc gcaccagccg
     1501 ctgcgccagc accgcgttca ccgccgcgct gatcaggtac ggctcaacgc cgatgttgat
     1561 caggcgcgtc accgaactgg gcgcgtcgtt ggtgtgcagc gtcgagagca ccatgtgccc
     1621 cgtcatcgcc gcctgcaccg cgatgtgcgc cgtctccgtg tcgcggattt cgcccagcat
     1681 gatcacatcc gggtcctgcc gcagcagcgc ccgcagggcc gccgcaaacg tcatcccgat
     1741 ccggtccatc acctgaatct ggttggcgaa ccccaggtgg tattccaccg ggtcctccac
     1801 cgtgctgatg ttcatcttcg ccccgtccat gcaccgcagg cacgaataga gcgtcgtcgt
     1861 tttgcccgat cccgtcggcc cggtcaccag aataatgccg ttcggccggt cgatctgctt
     1921 ccgcagaatc accagcatgt cttccgaaaa tcccaactgc tcgagcccca gcgtgatcga
     1981 ccggttgtcc agaatacgga tcacggtctt ctcgccatat tgcgtcggca ccgtactcac
     2041 gcgcaagtcc acgtggcgcc cgtgcaccat cgtgcggatg cgcccgtcct gcggcagccg
     2101 gcgctccgaa atgtccatgt tcgccatgat ctttaaccgc gacgataccg ccgcctgcat
     2161 cgccgccggc gggttcatcg cctcgaacag cacgccgtca atgcggaacc gcaccactag
     2221 ccgcttctcg tgcggctcga tgtgaatatc gctcgccccc tgcttgatcg cgtcaaaaat
     2281 caaaaagttc accagccgga tgaccgggct ctcgcccgac gccttcgcga gatccgtcag
     2341 ttcctctttt tcctcggcga cgatctcaag ctcttcctcg cccatgtcct tgatgatctc
     2401 gtcgaacttc tccccctcgc tgctggtgga attgcccgcc tcgatcgccg ccacgatgtg
     2461 gctcaggcag accaccacga ccttcacctg ctttaccgtg acgcgccgct tcacctcttc
     2521 cagcaagaat atgtccgccg gatccaccat ccccaggatc agcctcccct cgtccagccg
     2581 cacgccacaa cagccccgcg ccttcatgaa gtccgacggc aaatagtcaa acgcccgccg
     2641 gtccagcttc ttcgggtcaa tggcctcgaa ctccagcccc atcatctccg ccaccgcccg
     2701 ctgaatgtcc acctcgccca ccgcggccat ctcgtgcaga atctgcgaga ccttcttccc
     2761 ccggctgttg gcctgcaccg tccgcgcctg caacagcttg tccgcgtcca gcttcccctg
     2821 cgccgccagt atctcctcca cggtcttgcg cttccccgag tcgctgggct gcggcgacca
     2881 cagcaccgac aacgcctcct cgctcgacgg caccagcgcc accggcggca gctccgccag
     2941 ctccgtcgcc gcagactccc gctccatctc ctcgagccaa ttttctacct tgcttttcgc
     3001 catgcgtttg cttctttgaa gatgcgcgtg tcagtccctg gatcgatcca tccttcgatt
     3061 cattcaatgc ttattgctca gtggcaccgc gttcggtttc aattccacgt ccgtcggctt
     3121 gccgttgacg tccttgaacc gcagcacgag cctcccgggg tctatcgaaa gcacttccca
     3181 cattttcagg tgtgagccta ccttcaccat ctgatccgtc cccgagtcac tgaccagcgc
     3241 ggccgggttg ctgcccgtca tgaccatctg caagcggaag ccctgatagg ccatcgttgc
     3301 gtctagcaat tccttcgaag ccgccggcgg taacaccggc ccttcccgcg gcccgggagg
     3361 cggctccgcg accgtcgccg tcacctcgtg ctcgaacgga ttccgcgaca aatccaccac
     3421 tttgttggac gcctcgaaat accggttcaa catttcgatc agccacgccg tcccccgctt
     3481 gccggcgccg tcctgccccg tcttgctgcg caccagctct tgaatccgct cgtcgatctt
     3541 ctgtcgaacc ttgtcatcct cggccgacgc cgtccgcggc ttgttctgca atcccagcag
     3601 gtacagcacg cccatcgccg ttgcaaacgc cgccaccagg gtcagcgtgc tggaattgaa
     3661 ccgcgggcct ttggactcgc tgtattcctc gggcggcgtg atgtcgcccc cgacacccaa
     3721 cggcatctgc acgtcgtcat cgccgccggt tgtgctttct ttccattgtt gactcattgt
     3781 gccaccgcca cattctgatt ggactcgtaa taaatcgtca gttcaaaatc cgccacgatc
     3841 acgccgttct tcttctcatc gtggtccggc tcgatcttca tttccttgat cttcgtgagc
     3901 cggggcatct gctccacctg gctcaaaaat ttgtaaaacg ccgggtgaaa cggcccctcc
     3961 accaccatcc ggatcggctg ctcgttgtag ttgggcccct caatgggctt gccgttgcgc
     4021 acgctcttca ccgtcagctt gttctccttc gccgccttcc acacctcgtc gagcaccttg
     4081 tccatctccg tctgcgccgg cagtttgctg gtcagaaact cgatggcttt cttcagctgc
     4141 gtgatctcct cgggcatgtt ctgcgtcgcc cgcatcgcac tggccaggtc cgccagcttc
     4201 tgctgcttga gcttcgtgtc cgccactgcc tgggcacgct gttcgttcag cggcttgaac
     4261 agcagcggat acgacatcac cagcattccc agcaccagca ccacgtacaa aactgttctt
     4321 aatccaaatt gcatgtctca ccttcggtag gccaggaccg gttcaacgcc ggcccgtcaa
     4381 aaattctcac gatcctcagt tcttcgggct tgcttccacc agcggctcgc cgccgcgcag
     4441 gtccgcgtcc ggatgaatgt gcatctccag gttgaacttc cgcagcgggg cgtc
//
LOCUS       DATIMH010001137         3867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1138340, whole genome shotgun sequence.
ACCESSION   DATIMH010001137 DATIMH010000000
VERSION     DATIMH010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138340"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..2040)
                     /locus_tag="VLI90_19630"
     CDS             complement(187..2040)
                     /locus_tag="VLI90_19630"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HSV16483.1"
                     /translation="MRFRSTSRRSGGTTARHIEPLEQRQLLSVVAPSFATPVSYNIGT
                     QPSPSVPNVSQDGVATGDLNGDGKLDLAVVHSIDNTVNVLLNKGGGAFSTAVAYPLQS
                     TNSIWVRVADVNGDGKRDLIVLGSKNNNGLIGVLLGNGNGTFKPVVSYAAGGVARGGV
                     GIADFNGDGKPDLVVAQFAAIDSTHSAVDVLINNGSGTFKPFYTVAVPPAARSVAVGD
                     FNKDGKTDLAVADGLGVNNQLDGTYPAGVTIMLGNGHGSFSKAAQYNALPTPDAGSDG
                     HGGGDVVNPELITAGDLNGDGNLDLVLSLYDHNIDIYLGNGNGTFHSAVGADTGEYPR
                     SVAFVDVNHDGKKDLVVDNIGKQSASEPGSIAVLLGNGNGTVQPAIQFTPFNYPGWMT
                     AGDFNGDGRTDLAITRLFDGHSVNVMLGQPPVTVPAPTVTSITPTHGPTSGGTSVTIT
                     GTNFLHVTAVRFGSFNATSFTVKSATTIVATAPAESAATVNVTVTNIAGTSPVASADR
                     YTFTSLATLPAPVIFSPGSTAQPGPTITTLTPTFTWRPVTTPGVDGYQLNLYDLTSKK
                     FASFTINGASKSSFTLLAADALAKGHQFVWNLRVLSGGQSGPPSSYFYFQT"
     gene            complement(2158..>3867)
                     /locus_tag="VLI90_19635"
     CDS             complement(2158..>3867)
                     /locus_tag="VLI90_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16484.1"
                     /translation="GLHADTGWRSRWGDRILLLPVILLGVALLLRPYVTFVLQGIPAA
                     GDSLIDQRLVSVGWWLFLVLRFVAVVALARFLPSPFRGPVCGAIAATLVWLNPASVLD
                     SMGWPQWEVWLPPWFLLAALLVSVDWWLTAGLALGFGCMFKGQLLFVAPLFILCPLFA
                     GWIGRFTRIIAGLGTGMALVCWPWFVRNDSAKWYILSTVFAAGLICLVSIYREALWRH
                     LRATGRALVGRARAWRSGDDIGVAFDGSPYKWQTLTVIAGTVLMLCFRLEPAKVRWEL
                     VALGAVGLLVAWRQTLRSLPWIAVATASVFACILVHQLRWHQQPGLDYLNPWIAALTA
                     AIVLVPWFIPRRMFAGWMVFAFAASVWLGSLALNGSFSWWEVGFMYGTRKHDQMQLGG
                     RSLSNLSSILQQRYRWDLHDPAATVQLTPKSQPIDLEVRSLMATMFFLTLTIIAAAAA
                     VHLRRRDRRFLVTLVAAWMCFVALLPQLAARYTLFPAIVAAVLVAVSVPMSLFQVLLT
                     TIACMMLGNQVIRFNPNIAPIALQMSTPTHPDFGWTMVALALVLLFASMVQTPRGRDL
                     PVA"
BASE COUNT          726 a         1217 c         1286 g          638 t
ORIGIN      
        1 gcttctagaa gtacttgtag ctgcgacgcc tgcgtcgcct tgcttttcag aagcaaagag
       61 gcgacgcagg cgtcgcagct acaagtgctt ctggaagctc caaatgtcga acgactctcg
      121 aaatttcaaa ccaccctcac cctaaccctc tcccggagta cctggcgagg ggaccagagc
      181 cgcgcatcac gtctggaaat agaagtagct gctcggcgga ccggattgcc cgccgctcag
      241 gacgcgtagg ttccatacga actgatgccc cttcgccagc gcgtcggcgg ccagcagcgt
      301 gaagctgctc ttgctggcgc cgttgatcgt gaagctcgcg aatttcttcg acgtcaggtc
      361 atacaggttg agttgatatc catccacgcc gggggtggtg accggacgcc aggtgaacgt
      421 cggcgtcagc gtggtgatcg tcggtcctgg ttgtgccgtg ctgccgggcg agaagatgac
      481 cggtgccggc agcgttgcga gcgaggtgaa ggtgtagcgg tcggccgagg cgaccgggga
      541 tgtgccggcg atgttggtaa cggtgacgtt caccgtcgcc gccgactccg cgggggcggt
      601 cgcgacgatt gtcgtcgcgg atttcacggt gaacgacgtt gcgttgaagc tgccgaagcg
      661 caccgccgtc acgtgcagga agttcgtccc ggtgatcgtg acgcttgttc cgccactcgt
      721 cgggccgtgc gtaggcgtga tgctggttac cgttggcgcc ggcaccgtca ccggcggttg
      781 gccgagcatg acgttgaccg agtgcccatc gaacaggcgc gttattgcga ggtccgtgcg
      841 gccatcgccg ttgaaatcgc ccgcggtcat ccagccggga tagttgaatg gcgtgaactg
      901 aattgccggc tgcaccgtgc cgttgccgtt gcccagcagc accgcgatgg accccggttc
      961 ggatgcggat tgcttgccga tgttgtcgac gaccaggtcc ttctttccat cgtggttgac
     1021 atcgacgaac gcgaccgagc gcggatattc gccggtgtcg gcgcctacgg ccgaatggaa
     1081 cgtaccgttg ccattgccca ggtagatgtc gatgttgtgg tcgtacagcg agaggaccag
     1141 gtcgaggttg ccgtcgccgt tcaaatcacc ggcggtgatc aactcggggt tgacgacgtc
     1201 gccaccgcca tgaccatcgc tgccggcgtc cggcgtcggc agggcgttgt actgcgcggc
     1261 tttgctgaag ctgccgtgac cgttgcccag catgatcgtc acgcccgcgg ggtacgtgcc
     1321 gtcgagctgg ttgttgacac ccagcccgtc ggcgacggcg aggtccgtct ttccgtcttt
     1381 gttgaaatcg cccacggcca ccgatcgcgc cgctggtgga accgcgacgg tgtagaacgg
     1441 cttgaacgtg ccgctgccgt tgttgatgag gacatccact gcgctgtgcg tgctgtcgat
     1501 cgccgcgaac tgcgcgacga ccaggtctgg tttgccgtca ccgttgaaat ccgcgattcc
     1561 aacgccaccg cgcgctacgc caccggcggc atagctgacg acgggcttga acgtcccgtt
     1621 gccgttgccg agcaacacgc cgatcaaacc gttgttgttc ttgctgccca agacgatcag
     1681 gtcgcgcttg ccgtcaccat tcacgtcggc gacgcgcacc cagatcgagt tggtgctctg
     1741 cagcggatat gccaccgccg tgctgaacgc gccgccgcct ttgttcagca gcacgtttac
     1801 cgtgttatcg atggaatgga cgacggcgag atccagcttc ccatcgccat tgagatcacc
     1861 ggtggcaacg ccgtcctggc tcacgttcgg caccgacgga ctcggctgcg tgccgatgtt
     1921 gtagctgacg ggtgttgcga agctaggcgc aacgaccgag agcaactgcc gttgctccag
     1981 cggttcaatg tgacgcgcgg tagtaccccc tgaacgccgg ctggttgaac ggaaacgcac
     2041 agctcgctcc tagtgaaatg tcgaacggcg gcggcagcgc agtcgcgctg cgcgtgaaga
     2101 tttgccgtac cctcgcggcc attattcgat atgtccaccg ggaacggaat tgcaggatta
     2161 tgcgaccggc aagtcgcgcc cgcgcggggt ctgcaccatc gacgcgaaca gcagcaccag
     2221 cgccagcgcg accatcgtcc acccgaaatc cgggtgggtc ggcgtgctca tttgcagcgc
     2281 gatcggcgcg atgttcgggt tgaatcgaat cacctggttg cccagcatca tgcaggcgat
     2341 cgtcgtcagc agcacctgga acagcgacat cggcaccgac acggccacta gcacggcggc
     2401 gacgatcgcg gggaacagcg tgtagcgcgc cgccagctgc ggcagcagag cgacgaagca
     2461 catccacgcc gccaccagcg tgacgaggaa ccgccggtcc cgccgccgca aatgcaccgc
     2521 cgccgcggcc gcgatgatcg tcagcgtgag gaagaacatc gttgccatca gcgagcggac
     2581 ctccagatcg atcggctgcg actttggcgt cagctgcacg gtggccgcgg ggtcgtgcag
     2641 gtcccagcga tagcgctgtt gaaggatcga cgagagattg ctcaagctgc ggccgccgag
     2701 ttgcatctgg tcgtgcttcc gcgtgccgta catgaagccg acttcccacc aggaaaagct
     2761 gccgttgagc gcgagcgacc cgagccagac ggacgcggcg aaggcgaaga ccatccagcc
     2821 ggcgaacatt cgccggggga tgaaccaagg gactaggaca atggccgcgg tgagggcggc
     2881 gatccagggg ttcaggtagt cgagccccgg ttgttgatgc cagcggagct ggtgaacgag
     2941 gatgcacgcg aagacgctgg cggtggcgac cgcgatccac ggcagcgagc gcagcgtttg
     3001 ccgccaggca acgagcagac cgaccgcgcc gagcgcgacc agttcccacc gcactttcgc
     3061 cggctcgagg cggaagcaga gcatgagcac cgttccggcg atgacggtca gcgtctgcca
     3121 tttgtacgga ctgccatcga acgcaacgcc gatgtcgtcg ccgctgcgcc acgcgcgcgc
     3181 tcggcccaca agcgcgcggc cggtcgcgcg caggtgacgc cacagcgcct cgcgatagat
     3241 cgacacgagg cagatcaacc ccgcggcgaa gacggtgctg aggatgtacc acttggccga
     3301 gtcgttgcgg acgaaccagg gccagcagac cagcgccatg ccggtgccga gccccgcgat
     3361 gatgcgggtg aaccgcccga tccacccggc gaacagcggg cagaggatga acaagggggc
     3421 gacgaacagc agctgtccct tgaacatgca cccgaaaccg agcgccagcc cggcggtgag
     3481 ccaccagtcc acgctgacca gcagcgccgc cagcaggaac catggcggca gccagacttc
     3541 ccactgtggc caacccatcg agtcgaggac ggatgcgggg ttgagccaga caagcgttgc
     3601 cgcgatcgcg ccgcagaccg gaccgcggaa ggggctgggc aggaatcgcg ccagcgccac
     3661 gacggcgacg aaccgcagca cgaggaacag ccaccagccg acgctgacga gacgctggtc
     3721 gatcagcgaa tcgccggccg ccgggatgcc ttgcaggacg aacgtgacgt agggacgtag
     3781 aaggagtgcg acgccgagca ggatgacggg aagcagcagg atgcggtcac cccagcgcga
     3841 gcgccaacct gtgtcagcgt gcaatcc
//
LOCUS       DATIMH010001138         1760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2291450, whole genome shotgun sequence.
ACCESSION   DATIMH010001138 DATIMH010000000
VERSION     DATIMH010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291450"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..950)
                     /locus_tag="VLI90_19640"
     CDS             complement(201..950)
                     /locus_tag="VLI90_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HSV16485.1"
                     /translation="MPDAATPDVLVRCKDVCKAFGTTAVLRGISMDVRKGEVVCIIGP
                     SGSGKSTFLRCINALVPIDSGTIVVAGLDVHKADLDKRALRHKVGMVFQQYNLFPHRT
                     ALQNVMMAPVHVLKRPREEVEEQARALIAKVRLQGKEDAYPGELSGGQQQRVAIARSL
                     AMNPEVMLFDEVTAALDPETVGDVLGTIRQLAAEGMTCILVTHEMGFARELADEIYFT
                     DAGRIVERGSPEELFGAPRDPRTRSFLERIL"
     gene            complement(982..>1760)
                     /locus_tag="VLI90_19645"
     CDS             complement(982..>1760)
                     /locus_tag="VLI90_19645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HSV16486.1"
                     /translation="LIRAHRQPAGLRDGPNRTCTAAAGGGHLSAAKQTIFDVLLNWSP
                     YIFKGFATNVFISIVSMVFATVLGLLVGIGQVSTRRPVRSVCWAFTNFFRNAPWLVVL
                     FYCMLLLPFQFRLGDVAISVPDWAKATLGIAVAAAANMSDLVRGAIQSIPTGQWESAE
                     SLAFTRRQTLFLVILPQCIKRLLPPWMNLYALVMVSTPLCAIVGVNEIMTVAADILAA
                     NSRHDLLIPVYFYILVLFFLYCYPISRITHRLERRFAVIG"
BASE COUNT          356 a          546 c          552 g          306 t
ORIGIN      
        1 gttgctcatt cgaagtatct gtattgagat gacacgatcg ggtgcgcagc acccggcgcg
       61 agggctcgac gcgtactcgg ctcaatcggg cgtggcctcg aggaagctgg cggaatccag
      121 ggttacggca cgaaacgctc gcctagcgcc atccaatctg gacctgcgct tgggtggctc
      181 gggcatggcc ccttctgtgg tcacaggatg cgctccagga acgagcgagt acgtggatcg
      241 cgcggtgcgc cgaaaagttc ttctggcgat ccccgctcca caattcggcc cgcgtccgtg
      301 aaataaattt catcggcaag ctcgcgcgcg aagcccatct cgtgggtgac gaggatgcag
      361 gtcatcccct cggccgcaag ctgccgtatc gtccccaaca cgtcgcccac ggtttccgga
      421 tccagtgccg cggtgacttc atcgaacagc atcacctccg ggttcatcgc caggctgcgc
      481 gcgattgcca cgcgctgttg ttggcctccg gaaagctcgc cgggataggc gtcttccttg
      541 ccttggagcc tgaccttggc aatcaacgcc cgggcctgtt cctcgacttc ctcgcgcggc
      601 cgcttgagaa cgtggacggg cgccatcatg acgttctgca acgcagtgcg atgcggaaaa
      661 aggttgtact gctgaaacac catgccgacc ttgtggcgca gagcgcgctt gtccaggtcg
      721 gccttgtgca catccagtcc ggctacgacg atcgtaccgc tgtcgatggg cacgagtgcg
      781 ttgatgcagc gcaggaaggt cgacttgccc gagcccgacg gcccgatgat gcagacgacc
      841 tcgcccttgc gcacgtccat tgaaatgccg cgcaggacgg cggtcgttcc gaacgccttg
      901 caaacgtcct tgcatcggac cagcacatcc ggcgtcgccg cgtcgggcac tgcttgcggg
      961 cccccgaatg ccatggccgc gctagccgat caccgcgaaa cgtcgctcga ggcggtgtgt
     1021 gattcgcgag atcggatagc agtagaggaa gaaaagcacg aggatgtaga agtaaaccgg
     1081 gatgagcagg tcgtgccgac tgttcgcagc aaggatgtcc gccgccaccg tcatgatctc
     1141 attgaccccg acgatggcgc agagcggcgt cgacaccatc accagcgcgt acaggttcat
     1201 ccacggcggt agtagccgct tgatgcattg cggcaggatg acgaggaaga gcgtctgtcg
     1261 acgcgtgaac gcgagcgact ccgccgattc ccactggccc gtcgggatcg attggatggc
     1321 accacgcacg aggtccgaca tgttcgccgc ggcggcaacg gcgatgccga gcgttgcctt
     1381 ggcccaatcc gggacggaga tcgccacgtc gccaagacgg aactggaacg gcaacagcag
     1441 catgcaatag aagagcacga caagccaagg tgcgttgcga aagaagttgg tgaacgccca
     1501 gcagacggag cgcaccggcc gccgcgtgga gacctgcccg atgccgacca gcaatcccag
     1561 gaccgtggcg aacaccattg aaacgatgct gatgaagacg ttggtggcga atcccttgaa
     1621 gatatagggt gaccagttca gcaggacgtc gaagatcgtc tgctttgccg cgctcaaatg
     1681 cccacccccg gcagccgcag tgcacgttcg attcggccca tcccgtagac cagcaggctg
     1741 acgatgagca cgtatgagac
//
LOCUS       DATIMH010001139        10174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5760009, whole genome shotgun sequence.
ACCESSION   DATIMH010001139 DATIMH010000000
VERSION     DATIMH010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5760009"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..750)
                     /locus_tag="VLI90_19650"
     CDS             complement(97..750)
                     /locus_tag="VLI90_19650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSV16487.1"
                     /translation="MRPHRWRCFHFSALLAVATCAAACQPRGKPAPAPQLGAPGSLSG
                     DVARNTDRAREETDRAYTLIQDGKYTDAEPALKRALVADPMYGAARNDLGIVYYHLNR
                     LYEAAWEFQNAAKLMPRQGQPQNNLGLVMDRAGRTDDALRAYDKAVELEPDNSEYAGN
                     LARARIRTGLRDEVTRRLLEVVVARDARPEWVDWARFNLTRLRPSPSTLEGSPTTEP"
     gene            1034..1867
                     /locus_tag="VLI90_19655"
     CDS             1034..1867
                     /locus_tag="VLI90_19655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HSV16488.1"
                     /translation="MSSELPPSQAAQIRLVAVDLDGTLLNDSKKVSDQTVNALCGLPA
                     MGVRVVIASARPPRSVRQIYRDLKLDTLQINYNGALIWDEANQRAVFHRPLAAAIVRD
                     IVDFSRDLFEEVLVSCEVMDRWYTDRVDDPFTTETGRMFKPDVVAPVEEFCSIDATKL
                     LLLAEPQIIARLEPELVERYSDRVTILQTDDYLLQIMHPRAGKAAALHMVAAHYGVPM
                     EQVMAIGDAPNDVGMLQLAGVAIAMDNAHREVKKVADWIAPSNNDHGVHAALVRYGLC
                     E"
     gene            complement(1947..2717)
                     /locus_tag="VLI90_19660"
     CDS             complement(1947..2717)
                     /locus_tag="VLI90_19660"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HSV16489.1"
                     /translation="MKRVALITGGSRGIGFGVAQELARSGFEIAINGRRDASDVADAV
                     RTLESLGAAVLYCRADVAIADDRARMLAEVEQRFGRLDVLVNNAGVAPEVRADILDAS
                     EQSFDRLININLKGPYFLTQSAAKWMIRQREADANFRGCIVSVSSVSATVASTNRGDY
                     CISKAGIAMAVKLWAARLAEFNIDVFEVRPGVIATDMTAGVKEKYDKLFEQGLTVQKR
                     WGTPADVGKAVAVLARGELPYATGQVINVDGGMTMQRL"
     gene            complement(2780..4174)
                     /locus_tag="VLI90_19665"
     CDS             complement(2780..4174)
                     /locus_tag="VLI90_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16490.1"
                     /translation="MQIENKLTAKDLQKQIGHTLELAGRKARLLEKSWDTSKGTPVFT
                     VDGKYTTRGWTEWTQGFQYGCAILAFDGTGEKDLLELGRKNTVEKMAPHVTHVGVHDH
                     GFNNLSTYGNLRRLMREGRINHNEWELAFLELAIKSSGAVQAARWSGTPVGKTSPVIA
                     NSSSLGYIYSFNGPHSLFVDTMRSCRILGVAWQLGHKLMHENDRAADLLKRSVLHGLC
                     TNQYIVFHGDSTHTYDVRGRTSHEAIFNRADGNVRCRSTQQGYSPFSTWTRGLAWAML
                     GFAEELEFFATIEDAKFQQSVGLAKKDVVAAYERAAIDTCDHYINDCAAADGIVYWDD
                     GAPGLAKMGDWRARPADPFNEHEPVDSSASAIAAQGLLRLGKYLGQDRYTRAGLSIAR
                     VLMSEPYLATDENHQGLLLHSVYHRPNGWDHVPPGRKVPCGESSLWGDYHLVELAVML
                     QRLADEKPYLKFFD"
     gene            complement(4236..5063)
                     /locus_tag="VLI90_19670"
     CDS             complement(4236..5063)
                     /locus_tag="VLI90_19670"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSV16491.1"
                     /translation="MQTPAPLSKLCVHTITTKPLSLPEAIEAFRETNVAGITVWRDAL
                     VPLGVKESAKLLADSGLTIVSLCRGGFFPAVTAEGRQAAIDENKRIVDEAAAVGAPLI
                     VLVCGAVPGIPLNEQRKQIADAIAAVAPYAQERGVKLGIEPLHPMYAGDRSAVNTLGQ
                     ANDMVEQLRLPNVGVAIDVYHLWWDERLEAEIARCGRNRAIFAYHVCDWRAPTRDMLN
                     DRGLMGDGCIPLRQIRGWVEAAGFDGMIEVEIFSTELWATDQREYLRRIRDAYIDHV"
     gene            complement(5203..6351)
                     /locus_tag="VLI90_19675"
     CDS             complement(5203..6351)
                     /locus_tag="VLI90_19675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV16492.1"
                     /translation="METTKIGIIMNGVTGRMGGNQHLERSIAAIRKQGGVKVGNGSVI
                     MPEPILVGRNADKLKALSERYGGLPYSTDLNSLWGDKKYPVYFDAQTTQLRAEAVTKA
                     IAAGKHIYCEKPVATTSAEAIDLMRKAEKAGVKHGAVQDKLWLPGIRKLKYLIDTGFF
                     GKILSVRGEFGYWVFTGEDQPAQRPSWNYRAEDGGGIIIDMLCHWRYLVDNLFGNVNS
                     VSCLGATHIDQRIDENGKPYKCTAEDSAYATLVTDRNIVCHFNSSWCVRVRRDDLLTL
                     QVDGTNGSAVAGLRDCKIQHQSMTPKPTWNPDIDSPIKFFDAWQTVPTNQDYDNAFKV
                     QWEYFLRHVALNEPFPWTLREAAKGVQLAELSIESWKQRRWVDVPALA"
     gene            6460..7230
                     /locus_tag="VLI90_19680"
     CDS             6460..7230
                     /locus_tag="VLI90_19680"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSV16493.1"
                     /translation="MKRDVQFLFGQFVPRCAHRIDKHFNDYNVLQFIDGGAINLAIQD
                     ERFHLKGRWFFSSYPGPRIAFHAAPPHRTWVHRYLAFRGPAVRRWMSEGLFPIKPQAV
                     PTHAEYAGRFDEMLELSRRTDRWGAARAALAMESILIELAEERARPAATPAWLEATLA
                     RMQTMGGEVHQDDLAKEAGMPPRTFRRKFTAAVGTSPHEYQIACRIGHAKRMLGATEL
                     PIKTIAEQLGYRDVSFFTRQFRLVTGAPPATYRRSREG"
     gene            complement(7589..8941)
                     /locus_tag="VLI90_19685"
     CDS             complement(7589..8941)
                     /locus_tag="VLI90_19685"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HSV16494.1"
                     /translation="MGVFWKAEPMMSTTMDEVRGQSRLTIARRGIALIYLAMAMVLLV
                     ALLAFALDIGRLHLVKTQLQATADGAARAAALSVTQQTFNAGTGAVNNTAVAASSQDI
                     ASQNVLDGRTGSANASVIDTVIGYWSTTSRTFTAASGFTDYIQSNAVHVTVYCASSRG
                     NAVSQSFALVLGRGASRDESADAIAMASGYFPALGSGYGVFGVRSVDIKGNDSTNSYD
                     PTKPYGTTNRSSNGGVASDGLIKMNGNTTVRGDARWGPANSPYSTGGPSGGTVTGLKQ
                     ALPEMVNFPSVQAPASGAYDNAAIKNFLDTANPPNFSAGNTTAVNVPAGTYYVNDLSL
                     QANVTLNVTSGPVVFIVKGNVSLQGGTDTLSGLPGDLTIDVMPGANSTVDLGGGNNAY
                     LHLYAPDSDITTDGNNELFGWMIGKTVTMKGTNNVHYDGTTVSDGNPGHGKYTIALVK
                     "
     gene            complement(8941..9882)
                     /locus_tag="VLI90_19690"
     CDS             complement(8941..9882)
                     /locus_tag="VLI90_19690"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSV16495.1"
                     /translation="MSIEPQTVFLAGSGVSATLLVFAVSRMFVGGDQQRQVKRRMAQS
                     VSRDEATPRAERRDAPTSLLTRMTAAAAAPLQPRSEKDQSALKQRLGYAGQYAPAAAR
                     VLVGAKLLLLLAGVVAGYFIALAMEEPYPKSLAIPALLAAFGMMAPEMWLSYMIRREH
                     TRLEHALPDALDLLVVCVESGLTMDAALQRVSKEVALAHPSLARELAITHIETQMGVP
                     RSEAMRKLADRTGSKSLKSLTAMLIQAERFGTSIAGALRVYSESLRVKRQFQAQEMAA
                     RASVKLTFPLVFCIFPSLLTVAAGPALIQIMRTMHAW"
     gene            complement(9889..>10174)
                     /locus_tag="VLI90_19695"
     CDS             complement(9889..>10174)
                     /locus_tag="VLI90_19695"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSV16496.1"
                     /translation="TGGDLAEILDNIAKMIRNRIRLQQQVKALTSEGRATGWVLAALP
                     VGMFIVMSIMNPNYVSLLLNDPTGRMMLGSSAGLLVLGLVIIKRIVTIKV"
BASE COUNT         1798 a         3423 c         3054 g         1899 t
ORIGIN      
        1 caaagatgcg taaggtgcga aatggaacgc gaggcaaggc gaggcaacgt gtcggcgaag
       61 ccccgcatgt acacgcgggg cttcttctaa ccgactttac ggctcggtgg tgggtgaacc
      121 ttccaacgtc gaaggcgacg gccgcagtcg cgtgagattg aaccgcgccc aatcgaccca
      181 ttccggccgg gcgtccctcg cgaccacgac ctccaacagc cggcgggtga cttcgtcgcg
      241 cagccccgtg cggattcggg cgcgtgcaag gttgcccgcg tactcgctgt tgtccggttc
      301 caactcgacc gccttgtcat acgcgcgcag cgcatcatcc gtccgaccgg cgcggtccat
      361 caccaacccc agattattct gcggctgccc ctgccgcggc atcagcttgg cggcgttctg
      421 aaattcccac gccgcttcgt acaggcgatt gaggtggtaa tacacgatgc cgaggtcgtt
      481 tcgcgctgcg ccgtacattg gatcggcaac caatgcgcgt ttcagcgccg gctcggcatc
      541 ggtgtacttg ccgtcctgga tcaacgtgta ggcacgatcg gtttcctcgc gcgcccggtc
      601 cgtatttcga gcgacgtcgc cgctgaggct gcccggcgcg cccagctgcg gcgccggcgc
      661 cggcttgccg cgcggctgac aagcggccgc gcatgtggcg accgcgagca aggcgctgaa
      721 gtgaaagcat cgccagcggt gcggacgcat tcgatcatta tcggcacaac gtggccgttt
      781 ccggtactgc ccccggcaca cgcgcgcgct ccggtcccct cgcccggtac gccgggagag
      841 ggttagggtg agggtgattt ggattttcca gagacgctcg acgttcgaaa tcaccctcac
      901 cccgaccctc tcccgcagta ccgggcgagg gggacggacc agaaccgagc cacaaatgaa
      961 agtcatacct acgcttgtcg aacggcgtcg caagcttgca cgcgccgtgt tcctatctac
     1021 catggccccc gacgtgagtt ctgaattacc gccatcccaa gctgcccaga ttcgcctcgt
     1081 tgccgtcgat ctcgatggca cgctgctgaa cgattcgaaa aaagtcagcg atcaaacggt
     1141 caacgcgctg tgcggcctgc cggcgatggg cgtgcgcgtc gtcatcgcct cagcgcgccc
     1201 gccgcggagc gtgcggcaga tttaccgcga cctgaagctc gacacgctgc agatcaacta
     1261 caacggcgcg ctgatctggg acgaggcgaa ccagcgtgcc gtattccatc gcccgctcgc
     1321 ggccgccatc gtgcgcgaca tcgtcgattt ctcccgcgat cttttcgaag aggtgctggt
     1381 cagctgcgaa gtgatggacc gctggtatac cgatcgcgtg gatgacccgt tcaccaccga
     1441 gaccgggcgg atgttcaagc ccgacgtcgt cgcgccggtc gaggagtttt gttccatcga
     1501 cgcgacaaag ctgttgctgc tggccgagcc gcaaatcatc gcgcgcctgg aaccggaact
     1561 ggtcgaacgc tacagcgatc gcgtgaccat cctgcagacc gacgattacc tgctccagat
     1621 catgcacccg cgcgccggca aggcggcggc cctgcacatg gtcgccgccc attacggcgt
     1681 gccaatggaa caagtgatgg cgatcggcga cgcccctaac gacgtcggca tgctgcaatt
     1741 ggccggcgtc gcgatcgcga tggacaacgc gcaccgagaa gtcaaaaaag tcgccgactg
     1801 gatcgcgccg agcaacaacg accacggcgt gcacgccgcg ctcgtgcgat acggcctctg
     1861 cgaatgagtg ttgcgcccgc gtgagaatca cgccgggttc ggagcgtact cgcgacgacc
     1921 cggatgcttc acgcgaaatg gacaagtcac agccgctgca tcgtcattcc cccatccaca
     1981 ttgatcacct gccccgtcgc gtacggcagc tcgccgcgcg ccagcaccgc cacggccttc
     2041 cccacgtcgg ccggcgtgcc ccaccgcttc tgcacggtca gcccctgctc aaacagcttg
     2101 tcgtacttct ccttcacgcc cgcggtcatg tcggtggcga tcacgccggg gcggacttcg
     2161 aagacgtcga tgttgaactc cgccaaccgc gccgcccaca gcttcaccgc catcgcgatg
     2221 cccgctttgc tgatgcagta gtccccgcgg ttggttgaag cgacggtggc gctgacggat
     2281 gacacgctga cgatgcaccc gcggaaattt gcgtcggctt cgcgctggcg gatcatccac
     2341 ttggcggcgg actgcgtgag gaagtacggc cccttcagat tgatgttgat caaccgatcg
     2401 aagctttgct cgctggcgtc gaggatgtcc gcccgcactt ccggcgcgac gcctgcattg
     2461 ttcacaagca cgtcgagccg gccaaaacgc tgctcgacct cggcgagcat tcgcgctcga
     2521 tcatccgcga tggcgacatc agcgcgacag tacaggactg cggcgccgag cgattcgagc
     2581 gtgcgaaccg catcagcgac atcggaagca tcgcgccggc cattgatcgc gatctcgaac
     2641 ccgctgcggg ccagctcctg cgcgacgccg aagccaatcc cgcgactgcc gccggtgata
     2701 agtgcaacac gcttcattcg tagcacgggc gtctcgcccg tgtccaagca acttggcacg
     2761 ggcgagacgc ccgtgctatc taatcgaaaa acttcaaata cggcttctca tccgccaacc
     2821 gctgcagcat caccgcgagt tcgaccaaat gataatcccc ccacaagctc gactcgccgc
     2881 acggcacctt tcggccgggc ggtacgtggt cccaaccgtt gggccgatga taaaccgaat
     2941 gcagcagcag gccctgatgg ttctcatccg tcgccaggta tggctcgctc atcagcacgc
     3001 gcgcgatcga caatcccgcg cgggtgtatc gatcctgacc cagatatttc ccaaggcgaa
     3061 gcagcccctg cgccgcgatc gccgacgcgg atgaatccac cggctcgtgc tcgttgaacg
     3121 gatccgccgg ccgcgcgcgc cagtcgccca tcttcgccaa ccccggcgct ccgtcgtccc
     3181 aataaacgat gccatcggcc gccgcgcagt cgttgatgta atgatcgcac gtgtcgatcg
     3241 ccgcccgctc gtacgcggcc accacgtcct tcttcgccag gcccaccgac tgctggaact
     3301 tcgcgtcttc gatcgtcgca aaaaactcca gctcttcggc gaatccgagc atcgcccatg
     3361 ccaacccgcg cgtccatgtg ctgaacggcg aatacccctg ctgcgtgctg cggcaacgga
     3421 cgttgccgtc ggcgcgattg aagatcgcct catggctcgt ccggccgcgc acgtcgtacg
     3481 tgtgggtcga gtcgccgtgg aagacgatgt actggttcgt gcacagcccg tgcaggaccg
     3541 atcgcttgag caggtcagct gcccgatcgt tctcgtgcat gagcttgtgc ccgagctgcc
     3601 acgcgacgcc gaggatgcgg caggaccgca tcgtatcgac aaacagcgag tgcggcccgt
     3661 tgaacgagta gatgtagccg aggctcgaac tgttggcgat caccggcgac gtcttgccca
     3721 ccggcgtgcc cgaccaccgc gctgcctgca ccgcgccgct gctcttgatc gccagctcga
     3781 ggaacgccag ttcccattcg ttgtggttga tgcgcccttc gcgcatcaac cggcggagat
     3841 tgccgtacgt cgagaggttg ttgaacccat ggtcgtgcac gccgacgtgc gtgacgtgag
     3901 gggccatctt ctcgaccgtg ttcttccggc ccagttccag caggtccttc tcgccggtgc
     3961 catcgaaggc gaggatggcg cagccgtact ggaagccctg cgtccactcg gtccagccgc
     4021 gggtcgtgta cttgccgtcg accgtgaaga ccggcgtgcc tttggacgtg tcccaggact
     4081 tctccaacag gcgagccttg cggcccgcca gctcgagcgt gtgaccgatc tgcttttgaa
     4141 gatcttttgc agtgagttta ttttcgattt gcattgatgt agcacgggcg tctcgcccgt
     4201 gtcgaacggc tccaggcacg ggcgagacgc ccgtgctaca catgatcaat gtacgcgtca
     4261 cggatgcgcc gcaaatactc gcgctgatcc gtcgcccaca actccgtcga gaaaatttcg
     4321 acctcgatca tcccgtcaaa ccccgcggct tcgacccagc cgcgaatctg ccgcagcgga
     4381 atgcacccgt ctcccatgag cccgcggtca ttcaacatat ctcgagtcgg cgcacgccag
     4441 tcgcacacgt ggtacgcgaa gatcgcgcgg ttccggccgc agcgggcaat ttccgcttcc
     4501 agccgctcgt cccaccacaa gtgatacacg tcgatcgcga caccgacgtt cggcagccgc
     4561 agctgctcga ccatgtcatt cgcctgtccc agcgtgttca ccgcactgcg atcgcccgcg
     4621 tacatcgggt gcagcggctc gatgccaagt tttaccccgc gctcttgcgc gtacggcgca
     4681 accgccgcga tggcgtcggc gatttgcttg cgctgctcat tcaggggaat gccgggcacg
     4741 gcaccgcaga ccagaacgat cagcggcgcg cccacggccg ccgcttcatc gacgatgcgc
     4801 ttgttttcat cgatggcggc ttgtcgacct tccgccgtca ccgccggaaa gaatccaccg
     4861 cggcagaggc tgacgattgt caggccggag tccgccagca gcttcgccga ttccttcaca
     4921 cccagcggca caagcgcgtc gcgccacacg gtgatcccgg ccacgttcgt ctcacgaaac
     4981 gcctcaatcg cctccggcaa cgaaagcggc ttggtcgtga tcgtgtggac gcagagcttg
     5041 gatagtggag cgggtgtttg catgacatcg cccgtctgct cccctcgccc ggtactccgg
     5101 gagagggtta gggtgagggt ggtttaatat ctcgacggcc gttgtacccg aaatcaccct
     5161 caccccagcc ctctcccggc gtaccgggag agggggaaag agttaagcca atgcaggcac
     5221 atccacccac cgccgctgct tccacgactc gatcgacagt tccgccagct gcacaccttt
     5281 ggccgcctct cgcagcgtcc acgggaacgg ctcgttcagc gcgacgtgcc gcaggaaata
     5341 ttcccactgc actttgaacg cgttgtcgta gtcctggttc gtcggcaccg tctgccaggc
     5401 gtcaaagaat ttgatcgggc tgtcgatgtc cgggttccac gtcggcttgg gcgtcatcga
     5461 ctgatgctga atcttacagt cgcgcaatcc cgcgacggct gaaccgttcg tgccgtccac
     5521 ctgcaacgtc agcagatcat cccgccgcac gcggacgcac cacgatgaat tgaaatggca
     5581 gacgatgttc cggtcggtga ccagcgtggc atacgcgctg tcttcggcgg tgcacttgta
     5641 cggcttgccg ttctcgtcga tgcgctgatc gatgtgcgtc gcccccaggc acgacacgct
     5701 gttgacgttg ccgaacaggt tgtcgacgag atatcgccag tggcagagca tgtcgatgat
     5761 gatcccgccg ccgtcttcgg cgcgataatt ccagctgggc cgctgggccg gttgatcttc
     5821 accggtgaag acccagtacc cgaactcgcc acggacggag aggatcttgc cgaagaagcc
     5881 agtgtcgatc aggtatttga gcttgcggat gcccggcagc cagagcttgt cctgcaccgc
     5941 cccgtgcttc acgccggctt tctcggcttt gcgcatcaga tcgatcgcct cggccgatgt
     6001 cgtcgcgacc ggcttctcgc aataaatatg tttgcccgca gcgatcgctt tcgtcacagc
     6061 ctcggcccgc agctgcgttg tctgcgcgtc gaaatagacc ggatacttct tgtcgcccca
     6121 caggctgttc agatcggtcg aatacggcag cccgccgtag cgctccgaca gcgccttgag
     6181 tttgtccgca tttcgcccga ccaggatcgg ctcgggcatg atcaccgacc cgttgccaac
     6241 cttcaccccg ccctgcttgc ggatggcggc gatcgaccgc tccaagtgct ggttcccgcc
     6301 catccggccg gtcacgccgt tcatgatgat gccgatcttg gtcgtttcca tgcctcgtcg
     6361 ctcctgctgc tggtggaact gaacggccag aggttacgcc gctggcgcaa ctgcttccat
     6421 gcattatgag gccatctttg gtactattcg gccaacgata tgaaacgcga tgtccagttc
     6481 cttttcgggc agttcgtccc ccgctgcgcg caccggattg ataagcactt caacgattac
     6541 aatgtactgc aattcatcga cggcggcgcg atcaatctgg ccattcaaga cgaacgattc
     6601 cacctgaaag gccgctggtt cttctccagc tatcccggcc cgcgtatcgc ctttcacgcg
     6661 gccccaccgc accgcacgtg ggtgcatcgt tatctcgcgt ttcgaggacc cgccgttcgg
     6721 cggtggatga gcgagggatt gtttccgatc aaaccacagg cagtgccgac gcacgcggaa
     6781 tacgccgggc gattcgacga gatgctggaa ctatcgcgcc gcaccgaccg ctggggcgcc
     6841 gctcgcgcag cgctggcaat ggagtcgatc ctgatcgagc tggccgaaga acgcgcccgc
     6901 cccgccgcca ccccggcgtg gttggaagcc acgctggccc gcatgcagac gatgggcggc
     6961 gaggtgcacc aggacgatct ggcgaaggaa gccggcatgc cgccgcgcac ctttcgaaga
     7021 aaattcaccg ccgccgtggg cacgtcgccg cacgagtatc aaatcgcctg ccgcatcggc
     7081 cacgcaaagc gaatgctggg ggcgacggag ctgccgatca aaacgatcgc cgagcagttg
     7141 ggctatcgcg acgtgtcgtt cttcacgcgg caattcagat tggtcaccgg cgcgccgccg
     7201 gccacgtatc gccgcagccg cgaaggttga gccgacaata tctgctcgca atccccccac
     7261 gccgggttcg gagcgtactc gcggcgaagc gagattctgc ggcgcgatag ggaagagatc
     7321 cgggccgtcg gcgtacctcc gaacccggcg tgtctgctta tccccattca cgactggaaa
     7381 tgcgctcgcg ccatcactgc gccgcctgcg tcgccgccgc atgttaggtg gtaggcggtc
     7441 tgccgatcgc atccagcgtc gcctgcaggt cggcatcggt gatgttcggg tgacgcttct
     7501 tttcccctct tgcgctatac ctctaagaac caggccggga gagggggaac gcgcatgtgc
     7561 ggttgccctc ctgacggcaa acctaccgct acttcaccag cgcgatggtg tacttgccat
     7621 gacccggatt gccgtccgac acggtagtgc catcatagtg tacgttgttg gtgcccttca
     7681 tcgtcaccgt ctttccgatc atccagccga acagctcgtt gttgccgtcc gtggtgatgt
     7741 ccgaatcggg tgcgtacagg tgcaggtatg cattgttgcc gccacccaga tcgacagtgg
     7801 aattggcgcc cggcatcacg tcgatcgtca ggtcccccgg cagtccgctc aacgtatcgg
     7861 tcccgccttg cagcgagacg ttgcccttga cgatgaacac gaccggacca ctggtgacgt
     7921 tcagcgtcac gttcgcctgc aggctcagat cgttcacgta atatgtcccg gccggcacgt
     7981 tgacggcggt ggtgttgcct gcggaaaaat tgggcggatt ggcggtgtcg agaaaatttt
     8041 tgatggcggc gttgtcgtac gcgcccgacg ccggtgcctg caccgagggg aaattcacca
     8101 tctccggcaa cgcctgcttc agtcccgtga ccgtgccgcc ggagggaccg ccggtggaat
     8161 acggcgaatt cgcaggcccc cagcgcgcat cgccgcggac ggtggtgttg ccgttcatct
     8221 tgatcaaccc gtcggacgcg acgccgccat tgctgcttcg gttggtcgtg ccatacggct
     8281 tggtcggatc gtagctgttg gtggagtcgt tgcccttgat gtccaccgag cgaacgccga
     8341 acacaccgta gccgctgccg agcgcgggga agtatccgct ggccatcgcg atcgcgtccg
     8401 ccgattcatc gcggctggcg ccccggccca gcaccagcgc gaacgactgc gacaccgcgt
     8461 tgccgcgtga cgatgcgcaa tacaccgtga cgtgcaccgc gtttgattgg atgtaatcag
     8521 tgaacccaga ggccgccgtg aacgtgcgcg acgtggtgga ccaatagccg atgacggtgt
     8581 cgatgaccga ggcgttggcg gagccggtgc gaccgtccaa cacattctgc gaagcgatgt
     8641 cctgcgacga cgcggcaacc gcggtgttgt ttaccgcgcc ggtgccggcg ttgaaggttt
     8701 gctgcgtcac gctcaacgca gcggcgcggg cggcgccgtc ggcggtggct tgcagctgcg
     8761 tcttcaccag gtgcagccga ccgatgtcca gcgcgaacgc cagcagcgcg accagcagca
     8821 ccatcgccat cgcgaggtag atcagcgcta tgccgcgacg cgcgattgta agccgcgact
     8881 ggccgcgtac ttcgtccatc gttgtgctca tcatcggctc cgccttccag aaaacgccca
     8941 tcaccacgcg tgcatcgtcc gcatgatttg aatcagcgcc ggcccggcgg cgacggtgag
     9001 cagcgatggg aagatgcaga acaccagcgg gaacgtcagc ttcaccgacg cccgggcggc
     9061 catttcctgg gcctggaact gccgcttcac gcgaaggctt tcgctgtaga cccgcaacgc
     9121 gccggcgatg ctggtgccga aacgctcggc ctggatcagc atcgccgtca gcgatttgag
     9181 cgacttcgat ccggtgcgat cggccagctt gcgcatcgct tcgctgcgcg gcacgcccat
     9241 ctgcgtttcg atgtgcgtga tggccagctc gcgcgccagc gacggatgcg ccagcgcgac
     9301 ttccttgctg acgcgctgca aggcagcgtc catcgtcagg cccgattcga cgcagacgac
     9361 cagcaggtcc agcgcatcgg gcagcgcgtg ttcgagccgc gtgtgctccc ggcggatcat
     9421 gtacgacagc cacatctccg gcgccatcat cccgaacgct gccagcaatg ctggaatcgc
     9481 caggcttttc ggatacggct cctccatcgc cagcgcgatg aaataccccg cgacaactcc
     9541 cgccagcagt agcagcaact tcgccccgac cagcacacgt gccgcggcgg gagcgtattg
     9601 accagcgtag cccaggcgct gcttcagggc cgactggtcc ttctcgctgc gcggttgcag
     9661 cggcgcagcc gcggcggcgg tcatgcgcgt cagcagggag gttggcgcat cgcggcgctc
     9721 ggcgcgcggc gttgcctcat cgcgcgaaac ggattgcgcc atccgccgct tcacctgccg
     9781 ctgctgatcg ccgccgacga acatccggct gacggcgaag accagcagcg tcgcgctcac
     9841 accgctgccg gcgagaaaaa cagtttgtgg ttcgatcgac attgcgcctt acaccttgat
     9901 cgtgacgatc cgcttgatga tgaccagccc cagcaccagc agccccgccg acgacccgag
     9961 catcattcgg ccggtcgggt cgttcagcag cagactgacg taattggggt tcatgatcga
    10021 catcacgatg aacatcccca ccggcaacgc cgccagcacc caaccggtcg cccggccttc
    10081 gctggtcagc gccttcacct gttgctgcag gcgaattcga ttgcggatca tcttcgcgat
    10141 gttgtccagg atctccgcca gatcgccacc ggtt
//
LOCUS       DATIMH010001140         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1138881, whole genome shotgun sequence.
ACCESSION   DATIMH010001140 DATIMH010000000
VERSION     DATIMH010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138881"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VLI90_19700"
     CDS             complement(<1..549)
                     /locus_tag="VLI90_19700"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HSV16497.1"
                     /translation="MEPAVRHRLLLSLRSEQALGLRAIPRGTIEMPAEISEVPEPAPV
                     VPRVAPPQPASRPARSEPAMLYGQPATPPAKPVNPPTAPAFDAPSLTLAEKRARLAAM
                     EEKEVRTCPKCRLCQTRTQTVFGEGDPDAKIFFIGEGPGENEDLTGRPFVGRAGQLLD
                     KMIAAMGLRREQVYIANIVKCRP"
     gene            complement(964..1338)
                     /locus_tag="VLI90_19705"
     CDS             complement(964..1338)
                     /locus_tag="VLI90_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16498.1"
                     /translation="MNLTYAVDRLYEIGWLPEAGTELERLEDGRRFPSVLAVQREFAR
                     AGLELAIKQNLMFKCYRATWAPLGEPLEDGHASDERHGTVVGSCQREAAVYALAQLRQ
                     AQSERQLAMVGSEGNGQFAAAM"
     gene            complement(1529..1933)
                     /locus_tag="VLI90_19710"
     CDS             complement(1529..1933)
                     /locus_tag="VLI90_19710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV16499.1"
                     /translation="MYRDNTDGGLARPKYDVLIVEDEPISRKALTALISAKGYNADAA
                     PSGEDALKRSAQNGVPMIALVDLELPGMNGIELIKRLEALNPRVFPVLITAAAGDLVD
                     SLRAKHPVAYLRKPLDFDRLLNLISERRTLHS"
     gene            2235..>3315
                     /locus_tag="VLI90_19715"
     CDS             2235..>3315
                     /locus_tag="VLI90_19715"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HSV16500.1"
                     /translation="MSNKSGSNEARRYRPYAPANFRAQAHESAHAEQAADDQPKIAHP
                     LVNHDPPTFIDTADALAELIEHLRAAGSFAYDSEFIGELTYIPKLCLIQVASAQRVAL
                     IDPLVGLDLQPFWELVCDPSVEKIVHAGQQDIEPVYRNAGCRAANVFDTQICAGFIGL
                     AYPVALSKLVLELVSAKLGKGLTFTHWDQRPLTSMQLRYAADDVRYLVAVRDLIGKRL
                     DALGHSAWAKEECDTLCDPGQFGFDPETYFLRIRGANHLPARNQSVLRELTIWRDARA
                     REHNVPARSFLKDEVLLDLARTPVKSVDRLERVRGLPRPVEQAHGGEIVAVTQKGLST
                     PAEDLPQVKDVEPTPKQRFRADALWG"
BASE COUNT          602 a         1108 c          972 g          633 t
ORIGIN      
        1 cggccggcac ttcacgatgt tggcgatata aacctgctcc ctccgcaaac ccatggcggc
       61 gatcatcttg tcgagcagct gcccggctct tcccacaaat ggccggccgg tcagatcttc
      121 gttttcgccc ggtccttcac cgatgaaaaa gattttcgca tccggatcgc cttcgccgaa
      181 caccgtttgc gtgcgcgtct ggcacagccg gcatttggga caggtgcgaa cttccttctc
      241 ctccatcgcc gcgagtcgcg cccgcttttc tgccagcgtc aacgatggcg cgtcaaacgc
      301 cggcgccgtc ggcggattga ccggcttggc aggcggtgtc gccggttgcc cgtacaacat
      361 cgccggctcc gatcgcgcgg gccgcgatgc tggctgcggc ggcgcgacgc gcggtacaac
      421 cggcgctggt tccggcacct ctgaaatctc cgcgggcatt tcaattgtcc cgcgtggaat
      481 cgcgcgcagc cccaatgcct gctcgctgcg cagcgacaac agcagacgat gtcgtacagc
      541 tggttccatc tttcgcgatg cgccctgaac ttcgattggg ctgcacatcc tagaagcgcg
      601 cagcgcgtct cgcaacgcgc cattttttcg tgggcgcgcg ccgcgcatcg caactcgcgc
      661 cgcgatcaag accaactcac gcgaaaattt tttcgcccaa tctttttctt gacactcgtg
      721 gaagatggca gagaatacgc gcgagataga gagccaccac gggccgcaac agcgcgcgcg
      781 aattgtcgcc ccgcacactg cagcacccca cggtggctct ttcgtttgca actcgtggcg
      841 cgcgaggcgc gtaaaaaagc ccacggctct accgtgggac ttgagtgcac ttgagtgcat
      901 gacacagacg aatctcgctt tgtcgcgagt acgctccgaa tccggcgtgg ggcggaattc
      961 aattcacatc gccgccgcga actggccgtt gccttcgctg ccgaccatcg ccaattgccg
     1021 ctcgctttgt gcctgccgca attgcgccag cgcatagact gccgcttcgc gctgacagga
     1081 accgacaacc gtcccatgtc gttcatcgct ggcgtggccg tcttccagcg gttcgcccag
     1141 cggtgcccac gtcgcacgat aacatttgaa catcaggttc tgcttgatgg ccagctccag
     1201 tcccgcgcgg gcgaactcac gctgcaccgc caacaccgat gggaaccgcc ggccgtcctc
     1261 gaggcgctcc agttccgttc ccgcctcggg caaccaacca atctcgtaca atcggtctac
     1321 tgcgtaagtg aggttcacgg ctcggctcct tcacctgtgg gttctgctgg cgtatcgcga
     1381 ctcgcgcgtg cacgcgagta ggtacatatt cggctcctcg ccacgggtcg tcgcaaaatc
     1441 gcggggggac actgtcgccc cgctgcggtt gaatgtcaaa gcaaaaattc agatcgcgga
     1501 tgagcgtgga gacgcgtgct actgcccgct acgaatgcaa agttcgccgt tcgctgatca
     1561 ggttgagcaa gcggtcgaag tcgagcggct tgcgcaggta ggccaccggg tgcttcgccc
     1621 gcaatgaatc gaccaggtct cccgcggcgg cggtgatcaa taccggaaac acccgcgggt
     1681 tcagggcttc cagccgcttg atcaactcga tgccgttcat gccgggcagc tccaggtcca
     1741 ccagcgcgat catcggcacg ccgttctggg ccgatcgttt gagcgcatct tccccggacg
     1801 gcgcggcatc ggcgttatat cccttggcgc tgatcagcgc ggtcagcgcc ttgcgcgaga
     1861 tcggctcgtc ttcgacgatc agcacgtcgt atttcggccg agctaggccc ccatcggtat
     1921 tgtctcggta catcagcttc accagatcct cgccctgcgc cacggcaaat caggtgcccc
     1981 tccggataaa atatgcccaa ctgcatttat cctatctcca agcagctgag acggttaaat
     2041 gctgtcttgc tcagcttgct tccgggaacc cttaccctac agaaccccga caccacggtc
     2101 aggtttttag gacaaatggt cggtaagctg ccccacagga cgatcggggc tggctcgcaa
     2161 cgatacccct tccacccgtc gcatctgcgc ggcgatggcc gtatatttcc gcccagcaac
     2221 aaaggcagcg atgtatgtcc aataaaagcg gttccaacga agcccggcgt tatcgtccgt
     2281 acgccccggc gaactttcgc gcgcaggcgc acgagtcggc ccacgcggaa caggcggcgg
     2341 atgaccagcc gaagatcgcc cacccgttgg tgaaccacga cccgccgacg ttcatcgaca
     2401 ccgctgacgc cttggcggag ctgatcgaac acctgcgcgc cgctggctcg ttcgcgtacg
     2461 acagcgagtt tatcggtgag ctgacttaca tccccaagct ctgcctgatc caggtcgcca
     2521 gcgcccagcg cgtcgcgctg atcgacccgc tcgtcgggct cgacctgcaa ccgttctggg
     2581 aactggtctg cgacccatcg gtcgagaaga tcgttcacgc cgggcagcag gacatcgagc
     2641 ccgtctatcg caacgccggt tgccgggcgg ccaacgtgtt tgacacccaa atctgcgccg
     2701 gtttcatcgg gctggcttat cccgtcgcgc tgtcgaagct ggtgcttgaa ctcgttagcg
     2761 ccaagctcgg caaaggtttg acgtttactc actgggacca gcggccgctg acgtcgatgc
     2821 aattgcgcta cgccgccgac gacgtgcgct atctcgtcgc ggttcgcgac ttgatcggca
     2881 agcggctcga tgcgctcggc cacagcgcct gggccaaaga ggaatgcgac acgctgtgcg
     2941 accccggcca gttcggcttc gatcccgaga cctacttcct tcgcatccgc ggcgccaatc
     3001 atctgccggc ccgcaatcaa tcggtgctgc gcgagctgac gatctggcga gacgcccggg
     3061 cgcgcgagca caacgtgccg gcgagatcct tcctgaagga cgaggtgcta ctggacctgg
     3121 cgcgtacacc ggtgaagtcg gtcgatcgcc tcgagcgagt gcgcggcctg ccaaggccag
     3181 tcgagcaagc ccacggtggc gagattgtcg ccgtcacgca aaagggatta tcgacacccg
     3241 cggaggacct gccgcaggtg aaggatgtgg aaccgacgcc gaagcagcgc ttccgcgccg
     3301 atgcgctgtg gggcg
//
LOCUS       DATIMH010001141         1962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2010286, whole genome shotgun sequence.
ACCESSION   DATIMH010001141 DATIMH010000000
VERSION     DATIMH010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010286"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VLI90_19720"
     CDS             <1..291
                     /locus_tag="VLI90_19720"
                     /inference="COORDINATES: protein motif:HMM:NF018889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1425 domain-containing protein"
                     /protein_id="HSV16501.1"
                     /translation="QITFTNWGLQQAIKVSKVAPERVGAGQLKVTVELYNATNSDVPL
                     DYKYSFTDKAGVALENQTDWQFVKLPPRGFQAFSFTSTSPVDDFRVQLRPAE"
     gene            567..1661
                     /locus_tag="VLI90_19725"
     CDS             567..1661
                     /locus_tag="VLI90_19725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HSV16502.1"
                     /translation="MIAPPLSIDEPFRLQALRRTHLLDTPLEERFERLTRLAQRILQM
                     PIAALSLVDADRQWFKSIQGFKIAETSREISFCGHAILRDDVMVVPDARADVRFSDNP
                     LVAGEPHVVFYAGCPVRASDGSRIGSLCVIDQKPRTMSHEDLQALRDLAAIAENELSE
                     SMQRSVQQELMAQLDMVSRQAQVDSLTRVWNREAIFELLDTELARARRAGTGVGILMA
                     DIDFFKRINDTLGHAAGDVVLSQTARRILGTIREVDVLGRYGGEEFLVVLGPCNGEVG
                     TKIVAERICDRIGASPLVTEFGPIQVSVSVGAAYCETATAVTGEMLVQAADEAMYRAK
                     RAGRSRVECAVVNGGERSSLKTASLKLAAS"
     gene            complement(1642..1923)
                     /locus_tag="VLI90_19730"
     CDS             complement(1642..1923)
                     /locus_tag="VLI90_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16503.1"
                     /translation="MAKDFSHPPASSIQNNLFVRLRNFVASCFLPPYFLQESPESAEE
                     FFAEEFSPDVSLQCSAPASVDLRSTAKLFGKARGLATCERAGALTTPQV"
BASE COUNT          373 a          573 c          687 g          329 t
ORIGIN      
        1 cagatcacct tcacaaattg gggcctgcag caggcgataa aggtttccaa ggtcgcgccc
       61 gagcgcgtgg gggcggggca gttgaaggtg acggtggagc tgtacaacgc gaccaacagc
      121 gacgtgccgc tggactacaa gtattcattc acggacaagg ccggcgtggc gctggagaat
      181 cagacggact ggcagttcgt caagctgccg ccgcgggggt tccaggcgtt cagcttcacg
      241 agcacgtcac cggtggacga tttccgggtg caattgcggc cggcggaata accaggcgct
      301 gcgccgacat cagaagactt tcccaggccg ccttcgggcg gccttttttt gcggccatcg
      361 cttcagcaga agcccgaatt accggacggc cgggtccccg ttccgggtaa acgtggggcg
      421 atccttggcg tcgcggttgg atttaacttg ggtcagcggg ggcaaatgac gaagaaacag
      481 gggcgagttt tccggccgtc gcaccgcgcg gccggcggct ccgcgccccg cgcgcgacgc
      541 ttccgccctg ttcgacgagg ccatgcatga tcgcaccgcc attgagtatt gatgagccct
      601 ttcgcctgca ggcgttacgc cggacccatt tgctcgacac cccgctcgag gagcgcttcg
      661 agcgcctcac acgcctggcg caacggatct tgcagatgcc catcgccgcc ctctcgctgg
      721 tggatgccga ccggcagtgg ttcaaatcga tccaggggtt caagatcgcg gaaacctcac
      781 gcgaaatttc gttctgcggc cacgcaattc ttcgggatga tgtgatggtc gtgcccgacg
      841 cgcgggcgga cgtgcggttt tccgacaacc cgctggtcgc cggcgagccc cacgtggtgt
      901 tttatgccgg atgccccgtg cgcgcttccg acggcagcag gatcggcagc ctgtgcgtga
      961 tcgaccagaa gccgcggacg atgtcccatg aagatttgca ggcgctgcgc gacctggcgg
     1021 cgatcgcgga aaacgagctg tccgaatcga tgcagcgcag cgtgcagcag gagctcatgg
     1081 cccagctcga catggtgagt cggcaggcgc aggtggattc cctcacgcgc gtctggaacc
     1141 gcgaggcgat ctttgaactc ctcgacaccg agctggcccg ggcgcgccgc gcgggcacgg
     1201 gcgtgggcat cctgatggcg gacatcgatt tcttcaagcg gatcaatgac acgctggggc
     1261 atgccgccgg cgacgtggtg ctgtcccaga cggcgcggcg gatcctggga acgattcggg
     1321 aggtggacgt gttggggcgg tacggcgggg aggaattcct cgtggtgctg gggccttgca
     1381 acggagaagt gggaacgaag atcgtcgccg agagaatctg cgaccgcatc ggcgcgtcgc
     1441 cgctggtgac ggagtttggg ccgattcagg tgagcgtgag cgtgggggcg gcgtattgcg
     1501 agacggcgac ggcggtcacc ggcgagatgc tggtgcaggc ggcggacgaa gcgatgtacc
     1561 gcgccaagcg cgcgggccgc agccgggtgg agtgcgccgt ggtcaacggc ggcgagagga
     1621 gttcgctaaa gacggcgtcg ctcaaacttg cggcgtcgta agcgcacccg cgcgttcgca
     1681 cgttgccaaa ccccgcgcct tgccaaaaag tttcgccgtc gatcgaagat cgacgctcgc
     1741 cggggcggaa cactggagcg aaacgtcagg agaaaactcc tcggcaaaaa actcctccgc
     1801 agattccggc gattcctgca gaaaatacgg ggggagaaaa cacgaagcca cgaagttacg
     1861 aagacgcacg aagaggttgt tctggatcga ggaggccgga gggtgcgaga agtcttttgc
     1921 cacagagcca cagaggcaca gaggttttca cagagacgcg gg
//
LOCUS       DATIMH010001142         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2871486, whole genome shotgun sequence.
ACCESSION   DATIMH010001142 DATIMH010000000
VERSION     DATIMH010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2871486"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VLI90_19735"
     CDS             <1..753
                     /locus_tag="VLI90_19735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSV16504.1"
                     /translation="LTLTEGLLPYGYRVIAPSRFGYLRTDFPADASSEHQADALVELL
                     DRLHIEKLAVAAGSAGALSAVQFALRHPDRSSALILIVPAANVRGTDPVRMSAMQELL
                     VRRLTTSNLLFWVGIRAARDQMIGTLLATDPALVGRATPFEQRRADRILDEIMPVARR
                     SKGMLNDAKLAGNPARADFSKITVPTLVISVADDRFGTAATARDIAAAVPNSRLVIYP
                     QGGHIWIGHDDDLWREVARFLVESQEAAPGNA"
     gene            complement(804..>2316)
                     /locus_tag="VLI90_19740"
     CDS             complement(804..>2316)
                     /locus_tag="VLI90_19740"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HSV16505.1"
                     /translation="IQAEIVSMQRRLAAAPKAQHAELQTRISELQSELELVNARRNLE
                     ASVSQFTYQSDANGSGVSALKAHIDAIAASIPSSSGVGPAGPAAGSASGSVAPTTASS
                     PLGSGSDNASARLGIWDLAANVLRLSDKLRTIDTVDRRTAALEKTFTQIRTPPQEKIK
                     ALSARGEVLAEQADSSNGADLKGVRDQFDTLAWLFKQTAAILLPLSQEGVLLDQYRRN
                     LSSWRDNAQSQYFDALKALGVRVAILLGLLASVFAASEAWRRGVLRYVQEPRRRYQLL
                     LVRRIVCWGLVVAIVGFTFATDLGSLATFAGLLTAGVAVAMQSVLVSIVGYFFLIGKY
                     GIRVGDRVQIGSVTGEVIDLGLVRLHLMELSGQASLGPTGRVVAFANSIVFQASGGIF
                     KQIPGVNLAWHDITLTLPPEAEPYAMKEKLLAAANRVLGEYSEDIARQTREIQKTTSL
                     HAGGEAQAQVQLRFSASAVEALVRYPVQLQSAAEIDERISRELLKVITDAGGL"
BASE COUNT          405 a          763 c          734 g          414 t
ORIGIN      
        1 ctgacgttaa cggaaggact gctcccctac ggctatcgag tgatcgcgcc gtcacgcttc
       61 ggctatctcc gaactgactt tcctgcagac gcgtcgtcgg agcaccaagc cgacgcgttg
      121 gtggagttgc tggaccggct gcacatcgag aaacttgccg tcgcggccgg ctcggccgga
      181 gcactctccg ccgtccagtt cgcgttgcgc catcccgatc gcagctcggc cctgatcctc
      241 atcgttcctg ctgcaaacgt ccgcggaacc gaccccgtac ggatgagcgc gatgcaggag
      301 ttgctcgtgc gccgcctgac cacgtccaat ctgctgttct gggtgggcat ccgggcggca
      361 cgcgatcaga tgatcggcac gctgctggcg accgatccgg cactggtcgg gcgtgcaacg
      421 cccttcgagc agcgccgcgc tgatcgtatc ctcgacgaga tcatgcccgt cgcgcgacgc
      481 tcgaaaggca tgctcaacga tgcgaagctg gccgggaatc cggcccgtgc cgacttcagc
      541 aagatcaccg tccccactct ggtcatctcg gtggccgacg accggttcgg cacggctgca
      601 accgctcgcg acatcgccgc ggcggtgccc aattcgaggc tcgtcatcta tccgcaaggc
      661 gggcacatct ggattggcca cgacgatgat ctctggcgag aggtggcgcg cttcctcgtc
      721 gagagccagg aagcggcgcc tggcaatgcg tgatcccggg gcagccgcgg gcatgaaggg
      781 ttcatttagg cggtagtggc ctgttacagg ccaccagcgt ccgtgatcac cttcagcagc
      841 tcgcgcgata tgcgctcatc gatctccgca gcgctctgca gctgcacggg atagcgcacg
      901 agcgcctcga cggcggaggc cgagaagcgc agctgcacct gggcctgtgc ttcgccgcca
      961 gcatgcagtg acgtggtctt ctgaatttcg cgcgtttggc gagcgatgtc ttcgctgtat
     1021 tcgcccagca cccggttggc agcggcgaga agcttttcct tcatcgcgta gggctcggcc
     1081 tccggaggca gcgtgagcgt gatgtcatgc cacgccaggt tgacgccggg aatctgcttg
     1141 aagataccgc ccgaagcctg gaagacgatc gaatttgcaa acgcgacgac ccgcccggtc
     1201 ggcccgagcg acgcttgacc gctgagctcc atcagatgca gccgcaccag ccccaggtcg
     1261 atgacttcgc cggtcacgct gccaatctgc acgcgatcgc ccacgcggat gccgtacttc
     1321 ccgatgagga agaaataacc gacgatcgag acgagcacgc tctgcatggc tacggcgacg
     1381 ccggcggtga ggaggcccgc aaacgtagcc agcgacccga ggtcggtggc gaaggtgaag
     1441 ccgacgatgg ccacgaccag cccccagcaa acgattctgc gcaccagcag caactgatag
     1501 cgccgccgcg gctcctgcac gtagcgaagc actccgcgtc gccatgcttc cgaagccgcg
     1561 aataccgatg ccagcaatcc cagcaggatg gcgacgcgca ctcccagcgc tttcagtgcg
     1621 tcgaagtact gactctgagc gttgtcgcgc cagctgctga ggttgcgtcg atactggtcc
     1681 agcagcacgc cctcctggct caacgggaga agaatggcgg cggtctgctt gaacagccac
     1741 gccagggtgt cgaactgatc tcgcacgcct ttcaagtctg cgccattgga cgaatcggcc
     1801 tgctcggcga ggacctcacc gcgagccgac agcgccttga tcttctcctg tggcggagtg
     1861 cgtatctgcg tgaacgtctt ctccaaggcg gcggtgcggc ggtctacggt atcgatggta
     1921 cggagcttgt ccgacagccg cagcacgttc gcggcgaggt cccatatgcc gaggcgtgcg
     1981 gaagcgttgt cgcttcccga ccccagcggg ctggaggctg tcgtgggggc gaccgagcct
     2041 gacgcggaac ccgcggccgg acccgccggc cctaccccgc tcgaagaggg aatcgatgct
     2101 gctatggcat cgatgtgcgc cttcagcgcg ctgacgccac tgccgttcgc atcgctctgg
     2161 taggtgaact gcgagaccga cgcctccaga ttgcggcgcg cgttcacgag ttcgagctcg
     2221 ctttgcagct ccgagattct ggtttgcaac tcggcatgct gtgctttggg agccgcagcc
     2281 agacgacgct gcatggatac gatctccgcc tgtatc
//
LOCUS       DATIMH010001143         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5761075, whole genome shotgun sequence.
ACCESSION   DATIMH010001143 DATIMH010000000
VERSION     DATIMH010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5761075"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VLI90_19745"
     CDS             complement(<1..467)
                     /locus_tag="VLI90_19745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSV16506.1"
                     /translation="MSEATNDHSSLPLVYAVVPAFNRCAKTLRFLQLFKDVDYPNKRV
                     IVCDDGSTDNTFFNIRLNFPDVEVLRGNGHLWWSGGTNMAIRRALDAGADYILTINDD
                     SVMDPAFLREMVAIAQKNPKYIVGCRIHREDLPDRIWAIGTSPVFRGYEIFEL"
     gene            complement(525..>2040)
                     /locus_tag="VLI90_19750"
     CDS             complement(525..>2040)
                     /locus_tag="VLI90_19750"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSV16507.1"
                     /translation="SFIEKFNIDPAKIEVIPNAPQAELVNIPGDGIWTRQALPDRFLF
                     YPANTYRHKNHALLLDAIEQLNAAGVKMPVVFSGFELPGGFPLRKEIARRGLDGQCQV
                     FTDLPPQELRYLFRHALAVVMPTRFEGFGMPAIEAAACGCPLICSDLPVLREILDDNA
                     LYFSPDSVDEACAQIRRVMDDPSLREGLIERGFDIAERYTWDRSATRMLQVFKEARER
                     FVWSRHAPHTVKRPRIGILLRMNGHGGAGLVPTVESILCTGYPDLVTQCEVPADVPAH
                     TRQFLRSAGVRMIDAPKPSSNGNGDAHHRDGYDRLERFAREHNADLVTELFEGNRFKI
                     SGLDSAAWAYLEAPDKPVQLGEAMAWRGPQFVDIARFRLTGDGLWKLEGFLFTEMMFI
                     APAAMDAWTEGKQAIRDAGAEWRWELLRAAHSADRLFLTRRTLGDCDQHAVTAYAQAV
                     ASYAGINQYHTVATNQVARVRLLRYVEPGIKRAASVLPRKWQDAGTRLWYHLSR"
BASE COUNT          387 a          632 c          658 g          363 t
ORIGIN      
        1 ttcagctcga agatttcata gccgcggaag acgggcgagg tgccgatcgc ccagatgcga
       61 tcgggaagat cctcgcggtg gatgcggcag ccgacgatgt acttggggtt cttctgcgcg
      121 atggcgacca tctcgcgcag aaacgcgggg tccatcacgg agtcgtcgtt gatcgtgagg
      181 atataatcgg cgccggcgtc gagcgcgcgg cggatggcca tgttcgtgcc gccggaccac
      241 cacagatgcc cgttgccgcg gagaacttcg acatcgggga aattgaggcg gatgttgaag
      301 aacgtgttgt cggttgagcc gtcgtcgcaa acgatgacgc gcttgttggg atagtccacg
      361 tccttgaaca gctgcaggaa gcgcagcgtc ttcgcgcagc ggttgaacgc gggaacaacc
      421 gcgtagacca gcgggagcga actgtgatcg tttgtagctt cggacattgc tcaacaagcg
      481 cgcgcgactg tggcggcgcg gcccaaacgc ggcagtttac tgatttagcg agacaggtga
      541 taccacaacc gcgtgccggc atcctgccac ttgcgcggca aaacgctggc cgcccgttta
      601 atgcccggct cgacgtatcg cagcaaccgc acgcgcgcga cctgattggt cgcaaccgtg
      661 tgatattggt tgatcccggc gtagctggcc accgcctgcg cgtaagcggt gaccgcgtgc
      721 tgatcgcaat cgccgagcgt tcggcgggtc aaaaaaagcc tgtcggctga atgcgccgcc
      781 cgcagcaatt cccaccgcca ctcggcgccc gcgtcgcgga tcgcctgctt gccctcggtc
      841 caggcgtcca tggcggcggg ggcaatgaac atcatttcgg tgaacagaaa accttccaac
      901 ttccacagcc catctccggt cagacgaaag cgcgcgatgt ccacaaactg cgggccgcgc
      961 caagccatcg cctcacccag ctgaaccggc ttgtccggcg cttcgagata cgcccaggcg
     1021 gcggaatcga ggccggagat cttgaagcga ttgccctcga acagttcggt caccaagtcg
     1081 gcgttatgtt cgcgggcgaa acgctcgagc cggtcatacc catcgcggtg atgagcgtca
     1141 ccgttgccgt tggaggacgg cttgggcgca tcaatcatcc taacgccggc ggatctcagg
     1201 aactgccgcg tgtgcgctgg cacatccgcg ggcacttcgc attgcgtgac cagatcggga
     1261 taaccggtgc agagaatgct ttcgacggtg gggaccagtc ctgcgccgcc atgaccgttc
     1321 atgcgaagca ggatgccgat ccgcgggcgc ttcaccgtgt gcggggcgtg ccggctccag
     1381 acgaaacgct cgcgcgcttc cttgaacacc tgcagcatac gggtggccga ccggtcccac
     1441 gtatatcgct cggcgatgtc gaagccgcgc tcgatcagcc cctcgcgaag cgatggatcg
     1501 tccatcaccc ggcggatctg cgcgcaggct tcatcgacgc tgtcgggact gaagtacagc
     1561 gcgttgtcgt cgaggatctc acgcagcacc ggcaggtccg aacaaatcag cggacacccg
     1621 cacgcggcgg cctcgatggc gggcatgccg aagccttcga agcgcgtcgg catcacgacc
     1681 gccagcgcgt gacgaaacag gtaacgcagt tcctgcggcg gcaagtccgt gaagacctgg
     1741 cactgcccat ccagcccgcg gcgggcgatc tccttgcgca gcgggaaacc gccgggcaat
     1801 tcaaatccgc tgaagacgac cggcatcttc acgcctgcgg cgttcagttg ttcgatcgcg
     1861 tccagcagca gcgcgtggtt cttatggcgg tacgtgttgg cggggtagaa gagaaaccgg
     1921 tccggcaacg cttggcgcgt ccagatgccg tcgccgggga tgttcaccag ctcggcctgc
     1981 ggggcgttgg gaatgacctc gatcttcgcc ggatcgatgt tgaatttctc gatgaacgat
//
LOCUS       DATIMH010001144         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4603679, whole genome shotgun sequence.
ACCESSION   DATIMH010001144 DATIMH010000000
VERSION     DATIMH010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4603679"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1012
                     /locus_tag="VLI90_19755"
     CDS             92..1012
                     /locus_tag="VLI90_19755"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSV16508.1"
                     /translation="MASVDAPRIGRWETWGSAGCSPKVSGRQDVHPGFRQAFTLVELL
                     VVIAIITILVSLLLPALIRARSAAASAVCASNLHQLVTAAVNYAQDNQGYWPPANLNI
                     LTANLSRWHGNRPSAAASFDFITSLMRPYLRVDRIKTCPSWEAAVTKPGIAFEAGCGG
                     YGYNNHYLGTSTDVVSMQQSGLSPAKWDRLIGNVPAKQSMIQHPSAKLAFADTAMALA
                     GGVMIEYSFLEPPTSLLWSAGTVSWLTTSPSLHFRHSGRANVAWADGHVTSELYEWTY
                     PTNVYGADNKKCHLGFFGPHDDDSLFDREH"
     gene            1095..1910
                     /gene="lpxA"
                     /locus_tag="VLI90_19760"
     CDS             1095..1910
                     /gene="lpxA"
                     /locus_tag="VLI90_19760"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HSV16509.1"
                     /translation="MPQIHPTAVVDARAELGDDVEIGPYVIIQGHVVIGAGAIIHSHS
                     VVYGHSVIGRNCRIGPSAYIGMEPQHRSNAGVGTSCYIGDETTIRETAQVNRATRPGP
                     EHATRLGARCFLMAGAHVGHDCQIGDDVTMANAVLLGGHVTVGERVFFGGGSGVHQFC
                     RIGRLTVVAGIEQITRDVPPFAAVRYGGLKAYNAIGCRRFGLDRESIQAIRGAYRCLH
                     SNRTIPDAIAAIRATVPDVPVAAEIIAFATAKGRGLQPSVHFLNRLAAGDDSN"
     gene            complement(2045..>2513)
                     /locus_tag="VLI90_19765"
     CDS             complement(2045..>2513)
                     /locus_tag="VLI90_19765"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV16510.1"
                     /translation="REFAVSNFLPSMLSALQAHCSIPLIVNQVEIHLGRLDCFVDGTL
                     DQCLERQITPLSWSPLGGGWLGTGGKIKADDPKREMKERVQAAVDQQATKYGVSRSVL
                     SLAWLLKHPSGIIPIVGSNNPEHLRDATKADAVYLSREDWYQLLLAARGSALP"
BASE COUNT          453 a          838 c          746 g          476 t
ORIGIN      
        1 cgatccacgc cgggttcggg ggtactccaa cgactcggat tctgcggcgc gcgagaagca
       61 tccgggtcat cggagtagct ccgaacccgg cgtggcgagc gttgatgcgc ctcgtatcgg
      121 gcgatgggaa acttggggtt ccgctggttg ttccccaaaa gtttcaggtc gtcaggatgt
      181 acatcccggg ttccgccaag cgttcacgct cgtcgagctg ctcgttgtca ttgccatcat
      241 tacgatattg gtatctctcc tcctgcccgc gctcatccgc gcccgtagcg ccgccgcatc
      301 cgccgtgtgc gccagcaacc tccaccagct tgtcaccgcg gcggtcaatt acgcacagga
      361 caaccagggc tattggccac ccgcgaattt gaacatcctc actgcaaact tgagccgctg
      421 gcatggcaat cgtcccagcg ccgccgcgtc gttcgacttc atcacatcgc tcatgcggcc
      481 gtatctgcgg gtcgatcgaa tcaagacctg ccccagctgg gaagccgctg tgaccaagcc
      541 cggcatcgcc ttcgaagccg ggtgcggtgg ctatggctac aacaatcatt accttggcac
      601 cagcaccgat gtcgtttcga tgcagcagtc cggcctgagc cccgccaaat gggataggct
      661 gatcgggaac gtgcccgcca agcagtcgat gatccagcat ccatcggcaa agctcgcgtt
      721 cgccgacacc gcgatggccc tcgccggtgg tgtgatgatc gaatactcgt ttctcgagcc
      781 gcccacatca ctgctctgga gcgccggcac ggtcagttgg ttgacgacca gcccatcgct
      841 gcacttccgt cacagtggcc gggccaacgt cgcctgggcc gatgggcacg tcacgtcaga
      901 gctgtacgag tggacctatc cgaccaacgt ctacggcgcc gacaacaaga aatgccatct
      961 gggcttcttc ggcccgcacg atgacgattc gctcttcgac cgcgaacatt aatgcccatc
     1021 gcggcgtaag aattcgctgg gcacgcgctc gcgaatcgcg gcgtttgtct ctaaattgtt
     1081 cgtttcggag cgaaatgccg caaatccatc cgacagccgt cgtcgatgct cgtgctgaac
     1141 tcggcgacga tgttgaaatc gggccctacg tcatcatcca gggccacgtt gtcatcggcg
     1201 ccggcgccat catccactcg cacagcgttg tttacggcca cagcgtgatc ggccgaaatt
     1261 gccggatcgg tcccagcgcc tacatcggca tggagccgca gcatcggtcc aacgccggcg
     1321 tcggcacctc gtgctacatc ggtgacgaga ccacgattcg cgaaaccgca caggtgaatc
     1381 gcgccacgcg accgggccct gagcacgcga cgcggctcgg cgcgcgatgc tttttgatgg
     1441 cgggcgcgca cgtcggtcat gactgccaga tcggcgacga cgtcacgatg gcgaacgccg
     1501 tgttgctcgg cgggcacgtc accgttggtg agcgcgtctt tttcggcggc gggtcgggcg
     1561 ttcaccagtt ctgccggatc ggccgcttga ccgtcgttgc cggcatcgag cagatcacgc
     1621 gcgacgttcc acccttcgcc gccgtgcgct acggcggcct taaagcgtac aacgcaatcg
     1681 gctgccggcg attcgggttg gatcgcgaat cgatccaggc gatccgcggt gcgtatcgct
     1741 gcctgcattc caaccgtacg attcccgacg cgattgccgc catccgcgcg acagtgccgg
     1801 acgtgccggt ggccgcggag atcatcgcat tcgccaccgc caaaggccgc gggctgcagc
     1861 cgagcgtgca cttcctgaac cgcctcgccg cgggggatga ttcgaactag cgttagtggg
     1921 aaataccgcc cacgccgggt tcggagcgta ctcgcgacga agcgagattc tccggcgcgg
     1981 gcgaagcaac gcatccgggt tgtcggagta cctccgatcc cggcgtgttt gaatcatcag
     2041 ctggttacgg cagcgccgat ccacgcgccg ccagcagtag ctgataccaa tcctcgcgcg
     2101 acagatagac cgcgtcggcc ttcgtcgcat cccgaagatg ttcgggattg ttcgacccca
     2161 cgatcgggat aatgccgctc gggtgtttca gcagccacgc cagcgatagc acgcttcgac
     2221 tcacgccgta cttggttgcc tgctgatcca ccgccgcctg cacgcgctct ttcatctcgc
     2281 gctttggatc gtccgccttg atcttgccgc cggtgccaag ccaaccgccg cccaacgggc
     2341 tccagctgag cggcgtgatc tgccgctcga ggcactgatc gagcgtgcca tccacgaagc
     2401 aatccagccg gcccaggtga atctcgacct gattcacgat cagtgggatc gaacaatgcg
     2461 cctgcagcgc cgacagcatg gacggcagga aattactgac ggcgaactcg cgc
//
LOCUS       DATIMH010001145         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_852820, whole genome shotgun sequence.
ACCESSION   DATIMH010001145 DATIMH010000000
VERSION     DATIMH010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852820"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VLI90_19770"
     CDS             <1..862
                     /locus_tag="VLI90_19770"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSV16511.1"
                     /translation="LSEREHNPYGPLRTLVRPYILRRMKTDKRIIADLPDKTEVTAFC
                     GLTKPQAALYEQAVRELSSALTSADGIQRRGIVLAMLMRLKQICNHPAQLAAGAREYD
                     PKESGKFERLREICEELAERQEKVLVFTQFREITAPLTEFLGEVFGQPGLVLHGQTPV
                     GKRRQLVESFQSEEGPPFFVLSLRAGGTGLNLTEASHVIHFDRWWNPAVENQATDRAF
                     RIGQKRNVLVHKFVCRGTVEEKIDAMISEKVGLAKDLLDGTGEQLLTEMSNQELLKMV
                     SLDVNQALET"
     gene            1048..1935
                     /locus_tag="VLI90_19775"
     CDS             1048..1935
                     /locus_tag="VLI90_19775"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HSV16512.1"
                     /translation="MGWYYGGYGFRPYVSVAKRRAKAAKEAERFAKKGRVCCPIKIEG
                     RKIANTFWGKAWCDHLEQFSDFSNRLPRGRTYARNGSILDLQIEPGEVKALVMGSRLY
                     EVKIDVSPLAAELWKKVKCQCGGQIASLVELLQGRLSTSVMEIVTQRDRGLFPLPKQI
                     KLDCSCPDYASMCKHVAAVLYGVGARLDQQPELLFKLRGVDHLELIAGASDVTQLDKG
                     SSSRRKTIAADALSEVFGIDLASAPPPPDAREQRSPSSKATRRSKPDVIDEPRKTRER
                     PPAARKREPLIPRRKAKGT"
     gene            2149..3171
                     /locus_tag="VLI90_19780"
     CDS             2149..3171
                     /locus_tag="VLI90_19780"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HSV16513.1"
                     /translation="MVSGRLNWMGAALLAGMFIIAGCNKSDESTSSTATTQGSGAAAT
                     AGGKTLNLAFVTNNASDFWTIARAGTAKAMEEMPNVKVDFQIPSSGSAADQKRIVDDL
                     LTRGIDGIAISPVDPANETPMINQVAKQTLVFCHDSDAPNSDRACYVGTDNVAAGRQA
                     GDIVKQALPNGGKIMVFVGKRDAQNARERYQGLEESLKGSNVQILDVRTDDTDRARAK
                     SNVQETLVNYPDIAGLVGLWSYNGPAIYNAVKEANKAGQVKIVCFDEEDETLAGVKAG
                     AIYATVVQQPYEFGHMAITLMAKYLQGDKSVVPPTKQIFIPTLAITQDKVDAFKDNLD
                     KLRGRK"
BASE COUNT          669 a          930 c         1051 g          533 t
ORIGIN      
        1 actatccgag cgcgagcaca atccgtacgg cccgctgcga acgctcgtgc ggccatacat
       61 cctgcggcga atgaagactg acaagcggat catcgctgat ttgccggaca aaacagaggt
      121 gacggcgttc tgcgggctga ccaagccgca ggcggcgttg tacgagcagg cggtgcgcga
      181 gctgtccagc gcgctgacca gcgccgacgg catccaacgc cgcgggatcg tgctggcgat
      241 gctgatgcgg ctgaagcaga tctgcaatca tccagcgcaa ctggctgccg gagcgcgcga
      301 gtacgacccg aaggaaagcg gaaagttcga gcggctgcgc gagatctgcg aagagctggc
      361 cgagcggcag gagaaggtgc tggtcttcac gcagtttcgc gagatcaccg cgccgctgac
      421 ggaattcctc ggcgaagtct tcggccagcc ggggctggtc ctgcacgggc agacgccggt
      481 gggcaagcgg cggcagttgg tcgaatcgtt ccaaagcgaa gagggcccgc cattcttcgt
      541 cttgtcactt cgcgcgggcg gcaccggttt gaacttgacc gaggcgtcgc acgtgatcca
      601 cttcgaccgt tggtggaacc cggccgtcga aaaccaggcg accgatcgcg cgtttcgcat
      661 cgggcagaag cgaaacgtgc tggtgcacaa gttcgtctgc cgcggtacag tcgaggaaaa
      721 gatcgatgcg atgatcagcg agaaggtggg cttggccaag gacctgctcg acggcaccgg
      781 cgagcagctg ctgacggaga tgagcaacca ggaactgctg aagatggtgt cgctggatgt
      841 gaaccaggcg ttggagacct agctgccaga ggtaaacgga gcagggacga gaagtttgga
      901 accacagatg cacacgaatg cacacagatc gttctcttgt tgtacgcgcg agacgacagg
      961 gctgcgttct tatctgtgtt tatccgtgtg catctgtggt ttattgcctc cttctgcttt
     1021 gctgcgaaac tgaaaggagt cgcgcgcatg ggctggtact acggcggata tgggtttcgt
     1081 ccctacgtca gcgtcgcaaa gcgccgcgcg aaggcggcga aggaagccga gcgatttgcg
     1141 aagaagggcc gcgtctgttg cccgatcaag atcgaaggcc gcaagatcgc caacacgttc
     1201 tggggtaagg cgtggtgtga tcacctggaa cagttcagcg atttttccaa tcgtcttccg
     1261 cgcgggcgta catacgcgcg caacggatcg atcctggatc ttcagattga gccgggcgag
     1321 gtgaaggcgc ttgtgatggg ctccagattg tacgaagtga agatcgacgt ctcgccgctg
     1381 gcggcggagt tgtggaagaa agtcaaatgc cagtgcggcg gccagatcgc ttcgctggtc
     1441 gagttgctgc aaggccggct ctcgacatcg gtgatggaga tcgtcacgca gcgcgaccgc
     1501 gggctgttcc cgctgccgaa gcagatcaag ctggattgca gctgcccgga ctacgcgtcg
     1561 atgtgcaagc acgtcgccgc ggtgttgtac ggcgtgggcg cccggctgga tcaacagccg
     1621 gagctgttgt tcaagctgcg cggcgtcgat cacctggagc tgatcgccgg ggcaagcgat
     1681 gtgacgcagc tggacaaggg atcgagcagc cggcgaaaga cgatcgcggc ggatgcgttg
     1741 tcggaggtgt tcgggatcga tctagcgtct gcgccgccac cgccggacgc tcgcgagcag
     1801 cgctcgccat cgtccaaagc aactcgcaga tccaagcccg atgtaatcga tgaaccgcgc
     1861 aaaacgcggg aaaggccgcc agcggcacgc aagcgcgagc ccctgatccc gcggcgaaaa
     1921 gcaaaaggaa cttgaatagt tccagtaagt ggagtaatta gtatcccaac gccgttctcg
     1981 cgcggtcagg gtcagtggcg cgcgaatcat ttcggcggcg gagcgctttg gggtaaaact
     2041 cggttcgttc aggcgatgca gcgcacttcg cgctcggcgt gtgccggccc aacatcgggc
     2101 tcgcgcctga gatatcgata ggcggcgttc ttttcacgag gagacgacat ggtgtctgga
     2161 cgattgaact ggatgggcgc cgccttgctg gcgggcatgt tcatcatcgc gggttgcaac
     2221 aagagcgatg agagcacctc ctccaccgcc accacgcaag gcagcggcgc cgccgcgact
     2281 gccggtggca agacgctgaa cctggcgttt gtcaccaaca acgcatccga cttctggacc
     2341 atcgcccgcg ccggcaccgc caaggcgatg gaagaaatgc ccaacgtgaa ggtggatttc
     2401 caaatcccct ccagcggctc ggccgccgac cagaagcgca tcgttgatga cctgctcacg
     2461 cgcggcatcg acggcatcgc catcagcccg gtcgatcccg ccaatgagac gccgatgatc
     2521 aaccaggtcg ccaagcagac gctggtcttc tgccacgaca gcgacgcccc gaacagcgac
     2581 cgcgcgtgct acgtcggcac cgacaacgtc gccgccggcc ggcaggcggg cgacatcgtg
     2641 aagcaggcgc tgcccaacgg cgggaagatc atggtgttcg tcggcaagcg cgacgcgcag
     2701 aacgcccgcg agcgctacca aggtctggaa gaatcgctga agggatcgaa cgtgcagatc
     2761 ctggacgtgc gcaccgacga caccgatcgc gcccgcgcca aatcgaacgt gcaggaaacg
     2821 ctggtgaact atcccgacat cgccggcctg gtcgggctgt ggagctacaa cggtcctgcc
     2881 atctacaacg cggtgaagga agccaacaag gccgggcagg tgaagatcgt ttgcttcgac
     2941 gaagaagacg aaacgctggc cggcgtgaag gccggggcga tctatgcgac ggtggtccag
     3001 cagccgtacg agttcggcca catggccatc acgctgatgg ccaagtacct ccaaggcgac
     3061 aagtcggtgg ttccgccgac gaagcagatc ttcatcccga cgctggccat cacgcaggac
     3121 aaggtggacg cgttcaagga caacctggac aagctccgcg gccgcaagtg atctttctaa
     3181 cgc
//
LOCUS       DATIMH010001146         4822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2872378, whole genome shotgun sequence.
ACCESSION   DATIMH010001146 DATIMH010000000
VERSION     DATIMH010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2872378"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VLI90_19785"
     CDS             complement(<1..1248)
                     /locus_tag="VLI90_19785"
                     /inference="COORDINATES: protein motif:HMM:NF014948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HSV16514.1"
                     /translation="MSSKNAGITLGIIIGNRDFFPDVLVPEARKDITALLQAAGIASV
                     MLSPEQSKLGGVETHADARKCSELFRQQRDAIDGVLVLLPNFGDEKGVADALKLANLN
                     VPVLVQAYPDDLNALGVARRRDAFCGKISVCNNLKQANIPFTLTTRHVVHPTDPTFKA
                     DLARFVAVCRVVRGLRGARFGAVGARPAAFNTVRYSEKILERYGISVTTIDLSEVLGA
                     AGKLAASDARVKAKLDEINAYAPSQGVPAEKMLQMSRLGVVLDDFIAINALDSTAIQC
                     WTSLQQNWGCNVCTCMSMMSQKLMPSACEVDITGALSMYALSLASQRPSALVDWNNNY
                     GGDEDKCVLFHCGNWPKDFAPDTRIANAPILGSTLGVENTYGAMEGRNNAGPLTFARF
                     TTDDTAGVIRGYVGNGSTTDDPLN"
     gene            1528..2376
                     /locus_tag="VLI90_19790"
     CDS             1528..2376
                     /locus_tag="VLI90_19790"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSV16515.1"
                     /translation="MARFDPTRPDFTPYGLTVERWTPTLMQRPDRHNEVEINLLAHGS
                     LTYLLGGRKVTVQCGRITVFWAAIPHQIIRVEHNSSYHVATVPLAWFLQWDLPPRLVE
                     PILHGRMIAESRAKESAMDQAAFARWQRDLARPTDARRRVVLLEMQARLLRLALALPP
                     VGSTKNAKSPGVESPALNKAEEIACFLARNYHHPLTTDQVGKAVGLHPNYAMNLFKKV
                     FATTVTQYLTQHRLSHAQRLLVSSNGKILSIAMDSGFGSVSRFNDAFRKAFGCSPREY
                     RQEHRL"
     gene            2431..3552
                     /locus_tag="VLI90_19795"
     CDS             2431..3552
                     /locus_tag="VLI90_19795"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSV16516.1"
                     /translation="MDDAISSEPEFVGTTGASTAVAGRAVPAPLATVVAVRGLGRLFA
                     WAVLLLLFFWFKHIFTLVLMGFLAALCLSASLRPLLRWMPGPRGIKGFFIGLLPLCIC
                     AGLIFAAVWLLVAPIQRELQSWPQIEQRLDGWLAQMNEQMWLEPGRPMTVHELLRETG
                     SRFANSGSFRGIGGALTDTVVGLVFVFIGTIYMLTEPSDQSVRAVMALLPARRQEQTR
                     RALAALEPRLRYWFIGATVTAVIIGCTSLLGFWLIGVPFFIPLAILAGISEYVPTLGP
                     AFAFLVALIIASTQGKDEVLWLAGLYALVHVLESYVLIPFIYREAVRIPPVVTLFTVV
                     LWGGIFGIGGLILAIPIDLVLWTMAEAFIPGATEPLALD"
     gene            3741..>4822
                     /locus_tag="VLI90_19800"
     CDS             3741..>4822
                     /locus_tag="VLI90_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16517.1"
                     /translation="MNPANQTSQIVDRARSTSKCGVMICCELFLAIVLVWAGSARISL
                     AAAEAPATRPFMPWKSARVLLPNENGYGPIIGVLVSNDVAYVATGIYPINVNGFLTVL
                     KVPVNGEPVSVIGKIAAKAGPDSINMQVNNNVICLDDKDVFIRGGGDGVYDFPLDGRD
                     PCRIDTDPAFPSHNATALAVLNGQLYLALELSHPVETRPPEGMVKYTSRVRDGTWIVR
                     IDPKMKQMQVLAWSAQKEKALPMDGGPPVAWMAADVPRQRIVFFITAAKGNEKRSGFW
                     SLSASGAFKQVCPVIGSTYLERGGQPRDGKVSVGLNGCGITGLDFDLASDRPTVFYAS
                     QAVQWPKSIDDGLDRLHPRFSGNDFF"
BASE COUNT          842 a         1507 c         1545 g          928 t
ORIGIN      
        1 gttgagcggg tcatccgtcg tgctgccgtt gccgacgtat ccgcgaatca cgccagccgt
       61 gtcgtcggtc gtgaagcgcg cgaacgtcag cggcccagca ttgttccggc cttccatcgc
      121 gccgtaggta ttttcgactc ccagcgttga accgaggatc ggcgcgttgg cgatgcgcgt
      181 gtccggcgcg aaatccttcg gccagttgcc gcagtgaaag aggacgcatt tgtcctcgtc
      241 gccaccgtag ttgttattcc aatcgaccag cgccgacggc cgctgcgacg ccagcgacag
      301 cgcgtacatc gacagcgccc cggtgatgtc cacctcgcag gcgctgggca tcagcttctg
      361 cgacatcatg ctcatgcagg tgcagacgtt gcagccccag ttctgctgca ggctggtcca
      421 acattggatc gcggtggaat ccagcgcgtt gatcgcgatg aaatcatcca gcaccacgcc
      481 cagccgcgac atctgcagca tcttctcagc cggcacacct tgcgacggcg cgtaggcgtt
      541 gatctcatcg agcttcgcct tcacgcgcgc atcgctggcc gccaatttgc ccgcggcgcc
      601 gaggacttcg gatagatcga tcgtggtaac gctgatgccg tagcgctcga ggatcttttc
      661 gctgtagcgc accgtgttga acgcggcggg gcgagcgccg acggcgccga agcgcgcgcc
      721 gcgcaggcca cggacgaccc ggcagacggc gacgaacctt gccagatctg ccttgaatgt
      781 gggatcggtc ggatggacga cgtggcgcgt cgtcagcgtg aaggggatgt tcgcttgctt
      841 caggttgttg cagacggaaa tcttcccgca gaacgcgtcg cgccggcggg cgacgccgag
      901 cgcgttcagg tcatcggggt acgcctgcac caacacgggc acgttcaggt tggccagctt
      961 cagcgcatcg gccacgccct tctcgtcgcc gaagttgggc agcaggacga gcacgccgtc
     1021 gattgcgtcg cgctgctggc gaaacagttc ggaacatttg cgtgcgtcgg cgtgtgtctc
     1081 gacgccgccc agcttcgatt gctcgggaga cagcatgacg ctggcgatcc ccgccgcctg
     1141 cagcagcgcg gtgatgtcct tgcgcgcttc gggcacaagc acatccggaa agaaatcccg
     1201 gttgccgatg atgatcccaa gggtgatgcc cgcgtttttg ctcgacatga tcagaacctc
     1261 cagccgcgaa gaaggaagca tggacctttc ttagcgggga ctccacctgt ttttggtgaa
     1321 ttacttccta taaagtaaga tcggtgaagt agcacgggca agctgcccgt ggtgcgcgta
     1381 ctcgcgcacg gtcgatgtac gcatcgacac acaatagcgt cagcgacgtc tcttgaaagt
     1441 ccgtcgcttg cgcgattgct tcatcgacgg agtaccgtca atcttcgtgg agtaccacga
     1501 aacacgggcg agacgcccgt gctacgaatg gcgagattcg accccacccg ccccgacttc
     1561 acgccgtacg gcctcaccgt cgagcgctgg actccgacgc tcatgcagcg gccggaccgg
     1621 cataacgaag tcgagatcaa cctcctcgcc cacgggtcgc tcacgtacct gctcggcggc
     1681 cgaaaggtca ccgtgcagtg cgggcgcatc accgtcttct gggcggccat cccgcaccag
     1741 atcatccgcg tggaacacaa cagcagctat cacgtggcca ccgtgccgct ggcgtggttc
     1801 ctgcagtggg atctgccacc gcggttggtc gaaccgatcc tgcatggccg gatgatcgcc
     1861 gaatcgcgcg ccaaggaatc ggcgatggac caggcggcgt tcgcgcgctg gcagcgcgat
     1921 ctcgcccgcc ccaccgacgc caggcgccgc gtcgtgctgc tcgaaatgca agccaggctc
     1981 ttacgcctcg cgctggcgct gccaccggtt ggctcgacga aaaacgccaa atcccccggc
     2041 gtcgaatcgc ctgcgctcaa caaagccgaa gagatcgcgt gtttcctcgc gcgaaattat
     2101 caccacccgc tgaccaccga ccaggtcggc aaagccgtcg gcctgcatcc gaactacgcg
     2161 atgaacctgt tcaagaaggt cttcgccacc acggtcacgc aatacctcac gcagcaccgc
     2221 ctgtcgcacg cccagcggct gctggtcagc agcaacggca aaatcctcag catcgcgatg
     2281 gactccggtt tcgggtcggt cagccggttc aacgacgcgt ttcgcaaagc cttcggctgc
     2341 tcgccgcgcg aataccggca ggaacatcgg ctgtgagcgt tgcctcaccg cctcggctgc
     2401 cgcacgcgat ttgcttggtg accggcacgc atggacgacg ccatctcatc agaaccagaa
     2461 ttcgttggaa cgaccggtgc ctctaccgcg gtggccgggc gcgcggtgcc cgcgccgctg
     2521 gcgacggtcg tggcggtccg cgggctcggc aggctcttcg cgtgggccgt gctgttgctg
     2581 ctgttcttct ggttcaagca tatctttact ttggtcctga tgggattcct ggcggcgctt
     2641 tgcctgtcgg cgtccttgcg gccgctcctg cgctggatgc cggggccccg cggtatcaaa
     2701 ggattcttca ttggcctgct gccgctgtgc atctgtgccg ggctgatctt tgccgcggta
     2761 tggctgctcg tcgcacccat ccagcgcgaa ctgcaatctt ggccgcagat cgagcagcgc
     2821 ctggatgggt ggctcgctca aatgaacgaa cagatgtggc tcgaacccgg ccggcccatg
     2881 accgtccacg agctgctcag ggagaccggt agcaggttcg ccaacagcgg cagcttccgc
     2941 gggatcggcg gtgcactgac cgacacggtg gtcggcctgg tcttcgtctt catcgggacg
     3001 atctacatgc tgaccgaacc gagcgaccag agcgttcgcg cggtcatggc gctgctacca
     3061 gcgcggcggc aggaacagac ccgccgcgca ctggcggcgc tggagccgcg cctgcgctat
     3121 tggttcatcg gcgccaccgt cacggccgtc atcatcgggt gcacgtcgct gctcggcttc
     3181 tggttgatcg gcgtgccatt cttcattccg ctggccatcc tggccggcat cagcgaatac
     3241 gtgcccacgc tcgggccggc cttcgcgttt ctggtcgcgc tgatcatcgc cagcacgcag
     3301 ggcaaggacg aagtcctctg gctcgccggg ctgtacgccc tggtgcacgt gctggaatcg
     3361 tacgtgctga tcccgttcat ttaccgggaa gccgtgcgca tcccgccggt cgtcacgctg
     3421 ttcacggtgg tgctttgggg cggcatcttc ggcatcggcg gattgatcct ggcgatcccg
     3481 atcgacctgg tgctgtggac gatggcggag gcgttcatcc cgggcgcgac tgagccgctg
     3541 gcgctggatt gataccgtta tcctcagcgc aggcatactg gccggagtcg aagggtcttg
     3601 ccgaggagtc ggtggactgc tcggttgcga agcgcgagtc acagccgctt gcggcttggc
     3661 gtgtgtggta aattgaaaag gcgctaagcc gcaagcggcg gctcatttcc gatccccata
     3721 tgttacgata attgcttccc atgaacccag cgaatcagac cagccaaatc gttgaccgcg
     3781 cgcgatcgac gtcgaagtgc ggcgtgatga tctgctgcga actgttcctc gcaatcgtgt
     3841 tggtgtgggc aggcagcgcg cgaatttctc tcgcggctgc cgaggcaccg gcgacgcggc
     3901 ccttcatgcc ttggaagtcg gcacgggtcc tgctgccgaa cgagaacggc tatgggccga
     3961 tcatcggcgt gctcgtgtcg aacgacgtcg cgtatgttgc gacgggaatc tatccgatca
     4021 atgtcaacgg tttcctcacc gtgctgaagg tgccggtgaa cggggaaccg gtttcggtga
     4081 tcgggaagat cgcggcgaag gcggggccgg attcgatcaa catgcaggtg aataacaatg
     4141 tcatctgcct cgacgacaaa gatgtgttca tccgcggcgg cggtgacggc gtttacgatt
     4201 tcccgctgga cgggcgcgac ccgtgccgca tcgataccga tccggctttc ccatcgcaca
     4261 acgcgacggc gctggcggtg ctcaacgggc agctgtatct cgcgttggaa ctgagccatc
     4321 ccgttgagac tcggccgccg gagggaatgg tgaagtatac gagtcgagtg agggatggca
     4381 cgtggatcgt gcgcatcgat ccgaagatga aacaaatgca ggtgctggcg tggagcgcgc
     4441 agaaggagaa ggctctgccg atggatggcg gaccaccggt cgcgtggatg gccgcggatg
     4501 tgccgcggca acgcattgtg tttttcatta ctgcggcaaa gggaaatgaa aaacgcagcg
     4561 ggttctggtc gctgagcgcg agcggcgcgt tcaagcaggt ctgccccgtc atcggcagca
     4621 cgtacctgga gcgcggcggt cagccgcgcg acggcaaggt gagcgtgggc ttgaacgggt
     4681 gcgggatcac tggattggat ttcgatctgg ccagcgatcg ccccaccgtg ttctatgcga
     4741 gccaggcggt gcaatggccg aagtcgatcg acgacggctt ggatcgcctg catccgcgct
     4801 tcagcggaaa tgatttcttt ta
//
LOCUS       DATIMH010001147         1731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2011920, whole genome shotgun sequence.
ACCESSION   DATIMH010001147 DATIMH010000000
VERSION     DATIMH010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011920"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1283)
                     /locus_tag="VLI90_19805"
     CDS             complement(138..1283)
                     /locus_tag="VLI90_19805"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSV16518.1"
                     /translation="MANPKRQTILIDFIPVSEFQRQIVVGVARFCREHLGYRHRTLST
                     EVNLVSDAGEIDGVIAIVQTADPHHSSTRFGKPVVNISTIAYPVPFPTVTIDNRAVGK
                     MAAQHLIAQGYRSFGYHMESSVYFSRERGAGFIETLDQAGYGCSIFDTSTQADSRPAA
                     LNGSTTKWLTELPKPLGLFTHNDARASILVDLCRDARLSVPEEVGVIGVDNDPLLAHL
                     AAPGLSSIDPGAAMIGYRAAETLVRLLRAGEPPAPCVLLPPKGLVERESTEHSRFGDP
                     RVSAAVRFIRRHATLPVGVDDVVEAAGCSRRSLERLFRTNLGHTPAEEIRRVQVNHAK
                     WLLVESDRSLTQVSERCGYQSFRNFATAFRREVGISASTYRKKMRSA"
     gene            1441..>1731
                     /locus_tag="VLI90_19810"
     CDS             1441..>1731
                     /locus_tag="VLI90_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16519.1"
                     /translation="MRAKRGILAGLSIGCCLFGSEASAATLLYQWTFDNNGGTGTTVS
                     PNVTPNNLATDGGVLTMAKANGSSPIVFTPANLYTAGGGGVTGGASDYAFDNS"
BASE COUNT          341 a          519 c          550 g          321 t
ORIGIN      
        1 ctgctgagcg acgtgatcac cggcgttcaa ttcgtagatg gagtgaaaaa agccgcttga
       61 gctgtcgcgg ccatcaacaa cttttggaag tatctcggaa gcaaagcagg tctgcggtcg
      121 cgatgcggcg ggcatcatca cgccgaacgc atcttctttc gatacgtcga tgcgctgatg
      181 ccgacttccc ggcggaaggc ggtggcgaag ttgcggaagg attggtagcc gcagcgttct
      241 gagacttgtg tcagcgagcg gtcgctttcc accagcagcc atttggcgtg atttacctgc
      301 acgcggcgga tttcctcggc gggagtatgg ccgaggttcg tgcggaacaa ccgttccagc
      361 gatcgacgcg agcaaccggc ggcctccacc acatcgtcaa cgccgaccgg cagcgtcgcg
      421 tgcctgcgaa tgaatcggac ggcggcggaa acgcgcgggt cgccgaagcg cgaatgttcc
      481 gtcgattcgc gctcgaccag ccccttgggt ggaagcagca cgcagggcgc cggcggttcg
      541 cccgcgcgca gcaatcgcac cagtgtttca gccgcgcgat aaccaatcat cgccgccccg
      601 ggatcgatgc tgctgagccc cggcgcggcg aggtgggcga gcagcggatc gttatcgaca
      661 ccaatcaccc cgacttcctc gggcacgctg agccgggcgt cgcggcagag atcaaccagg
      721 atcgacgcgc gcgcgtcgtt gtgagtgaag agtcccagcg gttttggcag ctcggtcaac
      781 catttggtag ttgagccgtt caaggcagcc gggcgcgaat ccgcctgcgt cgaggtgtcg
      841 aagatcgaac agccgtagcc cgcctggtcc agggtttcga taaaaccggc gccgcgctcg
      901 cgcgaaaaat agaccgacga ttccatgtga tagccgaacg accggtaccc ctgcgcgatc
      961 aggtgctgcg ctgccatttt ccccaccgcg cggttgtcga tggtcacggt cggaaacgga
     1021 accgggtaag cgatggtgga gatgttcacc accggtttcc cgaaccgcgt cgagctgtga
     1081 tgtgggtcgg cggtttggac gatggcgatc acgccgtcga tctcaccggc gtcggaaacg
     1141 aggttaacct cggtggagag cgtgcgatga cggtatccga ggtgttcccg gcagaagcgg
     1201 gcaacaccga caacgatctg ccgctggaat tccgacaccg gaatgaagtc gatcaggatt
     1261 gtctgtcgct tcgggttcgc catcgcgtgc gcccagctta ccgtttggcc agatttgacg
     1321 caatcgcaga ctcattttga cgcattttcg ccttttatca ccgatgagaa cggataagct
     1381 gatctccata cagcgcttcg cagtgggaag cgctggcacg tcaaagacaa tggaggcgag
     1441 atgcgggcaa agcgtggaat tctggcgggt ctttctatcg gatgctgtct gttcggcagc
     1501 gaggcgtccg ctgcgacact cctgtaccag tggaccttcg acaacaacgg tggaaccgga
     1561 accacggtct cgccgaacgt aaccccgaac aacttggcaa ccgacggcgg cgtgctgacg
     1621 atggccaagg ccaacggcag ctcgccaatc gtcttcactc ccgccaacct gtacaccgcc
     1681 ggcggtggcg gcgtcaccgg tggcgccagt gactacgcct tcgacaattc c
//
LOCUS       DATIMH010001148         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_853466, whole genome shotgun sequence.
ACCESSION   DATIMH010001148 DATIMH010000000
VERSION     DATIMH010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853466"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VLI90_19815"
     CDS             complement(<1..535)
                     /locus_tag="VLI90_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16520.1"
                     /translation="MKRYFLLLIVLICAPAHGAGRDEPLAMTVSPQGSLTVSLNSKDL
                     LTGNWALRYWRAEKLDDGELALGEIQQTTATQDAPDHATVTHTYATAVAAYDYRLHGE
                     DLTISVHVKNNDAAKTLKMARFLGLTFHFARPPTGIIKSFHGSYLDAHGSHGELLYHP
                     STDSPLGAVYAADDSFGF"
     gene            complement(532..2118)
                     /locus_tag="VLI90_19820"
     CDS             complement(532..2118)
                     /locus_tag="VLI90_19820"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSV16521.1"
                     /translation="MTWNYILLWFAGSSCVVNLINASRASPPLRGWVGVSAFILAIAA
                     FLYYLHPDIAGLTAGSLWMALVLAPALLQRRIARLTVAQQYATGWRLASVVRWLHPFD
                     GVWQQARLLRALDVAESGSLDEAAAMLEQVASDAPPRVARAATGHRFRLLGEWPQLIA
                     FVERSASARSLQRDPQLLSAYLRALGETGQINEMLATIERYRDALDSPAFTNVRHMCR
                     LQAFVFAGEPDHVQHILTGPLRVLPPVAQQYWIGTAELAAGQIEAGTGRLLTIEESAT
                     PGARRAIARRLAQPPAIARPILLPQAIDVLRRLDRERDQEERFATIRTAGRRPWVTYG
                     IILANAAMFVAEWYFGGTTSEAAHTRLGAFHPDEMREGQYWRLLTANFLHFGIAHILL
                     NMLALFVLGPFVEFAIGGALYLLIYLASGVLAIGTVWVFQLLGWMQPDWLVGASGAIM
                     GLIGATAAIQLRGWWRERARVAWRRLRLLGLILVMQAIFDAMTPEVSGTAHLAGAAWG
                     FVLCSIVPHRTTSRRRQDSA"
     gene            complement(2372..>3451)
                     /locus_tag="VLI90_19825"
     CDS             complement(2372..>3451)
                     /locus_tag="VLI90_19825"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSV16522.1"
                     /translation="PVGAGTDNNGIQVAANLLDNPAANGAGNPAFAFGTHSYTGYNLA
                     PNFAGATLASRTIANALTNYSVPAQATVEGGAHAICVYGVSTNGVAANNQNYTINGFL
                     IHDPWTGYVASQLSVGNAAPLAAGGWGLGFNTYLRYGYDVVPGGALTQLPNGNVVPVR
                     LGAWFNYFNPSGGQNGAGPVFAGPGMKFTVEPLGPELPDTGDPLNDGGLPTPPNEIPE
                     LNASQAGADASSDLAADATLNTEAGLSGGSLDLADEVLMQMPGDVSGQGDWLVPYDGP
                     SGINDVTGAVMIDAATGVIDEATWLDPSDGLPSLTLAQLDQMFANQASGLHPNDNPVP
                     EPGSMMLVGVGAIVLNLRRRRARCR"
BASE COUNT          633 a         1101 c         1144 g          573 t
ORIGIN      
        1 caaagccgaa tgaatcatcc gcggcatata cagcgcccag cggtgaatct gtgctcgggt
       61 gatagagcag ttcaccatga gatccatgcg cgtcgagata actgccgtgg aaagatttga
      121 tgattcccgt cggcggccgc gcgaagtgga acgtcagccc gaggaagcga gccatcttca
      181 gcgtcttcgc ggcgtcgttg tttttgacat gaacggagat cgtcaggtct tcgccatgca
      241 ggcgatagtc atacgcagcc accgcggtgg cgtaggtgtg cgtcaccgtc gcgtggtccg
      301 gcgcatcctg cgtcgcggtc gtctgctgga tttcacccag cgccaactcc ccatcatcca
      361 gcttctccgc gcgccaatag cgcaatgccc agttgcctgt gaggaggtct ttagaattga
      421 gggataccgt gagcgagcct tgcggtgata ccgtcattgc cagcggttcg tcgcgcccag
      481 ctccatgcgc tggcgcgcag atcaacacga tcagcagcag gaaatatcgc ttcatgccga
      541 gtcctgtcgc cggcgcgatg tcgtgcgatg cgggacaatg ctgcacagca cgaaccccca
      601 ggccgccccg gcgagatggg cggtgccgct gacttctggc gtcatcgcgt cgaagatcgc
      661 ctgcatgacg aggatcagtc ccagcaggcg caatcgccgc caggcgacgc gggcgcgctc
      721 gcgccaccag ccgcgcagct ggatcgccgc ggtggcgcca atcagaccca tgatcgcgcc
      781 cgaagcgccg acgagccagt ccggttgcat ccagccgagc aattggaaca cccacacggt
      841 gccgatcgcc agcacaccgc tggccagata gatcagcagg tacagcgcgc cgccgatcgc
      901 gaattcgacg aacgggccga gcacgaacag cgccagcatg ttcaacagga tgtgggcgat
      961 gccgaagtgc aggaagttcg cggtgagcag ccgccagtat tgtccttcgc gcatctcgtc
     1021 aggatgaaac gcgccgagcc gcgtgtgagc tgcttcgctg gtcgtgccgc cgaaatacca
     1081 ctcggccacg aacatagcgg cgttggccag gataatgccg tacgtcaccc aaggccggcg
     1141 tcctgcggtg cggatcgtcg cgaagcgttc ctcctggtcg cgctcgcgat cgagccggcg
     1201 cagcacgtcg atcgcctgcg gcaacaggat tggccgcgcg atcgccggcg gttgcgcaag
     1261 ccgtcgcgcg atcgcccggc gagcgccggg tgtggccgat tcttcgatcg tcagcaaccg
     1321 accggtcccc gcctcgatct gccccgcggc cagctccgcg gtgccgatcc aatactgctg
     1381 cgcgacaggc ggcagcacgc gcagcgggcc ggtgaggatg tgctgaacgt ggtcgggttc
     1441 accggcaaac acgaacgcct gcagccggca catgtgccgg acgttcgtga acgccgggga
     1501 atcgagcgcg tcacgatagc gctcgatcgt cgcgagcatc tcgttgatct gcccggtctc
     1561 accgagggca cgcaggtacg cgctgagcag ttgcggatcg cgctgcaggg atcgtgcgct
     1621 ggcggagcgc tcgacgaacg cgatcagctg cggccactcg ccgagcaaac gaaaccgatg
     1681 cccggtcgcc gcccgcgcga cccgcggtgg cgcatcgctg gcaacctgtt cgagcatggc
     1741 ggcggcttcg tcgaggctgc cgctctccgc gacatcgagc gcccgcagca gcctggcctg
     1801 ctgccacacc ccgtcgaacg gatggagcca gcgaaccacc gacgccaatc gccatcccgt
     1861 ggcgtactgc tgcgcgacgg tcagtcgtgc gatgcgtcgc tgcaacagcg ccggcgccag
     1921 caccagcgcc atccacagcg atccggcggt caaacctgca atgtcaggat ggaggtaata
     1981 gaggaacgcc gcgatggcga ggatgaacgc gctcacgcca acccagcccc gcaacggcgg
     2041 cgacgcgcgg ctggcattga tcaggttaac gacgcaggaa ctgcccgcga accagaggag
     2101 aatgtaattc cacgtcatcg tcagcctgtc catcacaaaa ccgcgctgca gaaatgagct
     2161 tagccgctgg ggcgcggaag ctccaagggt cggacagaga gaagcggcgc atcgatgatg
     2221 cgtggaaggc agcagacaaa cgcagagcgc gccgaggtca ggttaatagc ccaggccgga
     2281 aggccgggga tcggattgcg aacattgcca attcgccgat cgatccccgg ccttccggcc
     2341 tgggctattg aagatttgat aggcctctag atcagcgaca acgcgcgcga cgtcggcgga
     2401 ggttgaggac gatcgccccc actccaacta gcatcatcga ccccggctcc ggcaccgggt
     2461 tgtcgttcgg atgcaacccc gacgcctggt tggcgaacat ctggtcgagt tgggcgagcg
     2521 tcagcgacgg caggccatcg ctgggatcga gccaggtcgc ctcgtcgatc acgccggtgg
     2581 cggcgtcgat catcacggcg ccggtcacat cgttgatgcc actggggccg tcgtacggaa
     2641 cgagccaatc gccctgcccg ctgacgtcgc cgggcatttg catcaggacc tcgtcggcca
     2701 gatcgagact tccaccggag aggccggctt cggtgttcag cgtggcgtcg gcggcgagat
     2761 cgctgctcgc gtcggcgccg gcttgtgatg cattgagttc cggaatttca tttggcggag
     2821 tcggtaagcc gccgtcgttc agcggatcgc cggtgtctgg cagctccggg ccaagcggtt
     2881 caaccgtgaa cttcatgccc ggcccggcga agaccgggcc cgcgccattt tgcccaccac
     2941 ttggattgaa atagttgaac cacgccccga ggcgcaccgg cacgacgttg ccgttgggca
     3001 gctgcgtcag cgccccgccg gggacgacgt cgtagccgta gcgaaggtag gtattgaaac
     3061 ctaatcccca tccaccggcg gccagcgggg cggcgttgcc gacggacagt tggctggcaa
     3121 cgtaaccggt ccagggatcg tgaatcagaa agccgttgat cgtgtagttc tgattgttgg
     3181 cggcaacgcc gttggtcgag acgccgtaca cgcagatcgc gtgggcgccg ccttcgaccg
     3241 tcgcctgcgc gggaacgctg tagttggtca gcgcgttggc gatggttcgg ctggccagcg
     3301 tggcgccggc aaaattcggc gcgaggttgt atccggtgta ggagtgcgtg ccaaaagcga
     3361 aggcgggatt tcccgcgccg ttggcggccg ggttgtcgag tagattggcc gcgacttgaa
     3421 tgccgttgtt gtcggtgccc gcgccgacgg g
//
LOCUS       DATIMH010001149         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1431909, whole genome shotgun sequence.
ACCESSION   DATIMH010001149 DATIMH010000000
VERSION     DATIMH010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431909"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..>1635)
                     /locus_tag="VLI90_19830"
     CDS             complement(206..>1635)
                     /locus_tag="VLI90_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16523.1"
                     /translation="PKSGGLNHITVNGDYVVQNLDARGLKIDRIEGKASLDAGVLKLA
                     PVAATYNAGRLDAAISYELSNPRHIFTDVKIDKWPYGTTGGATAALTAATKLDIDLEH
                     KGASGPLSATVDLQYHNAPLSHVDLVANLQGRMLAVQKIAGNLLSGTFDGAATVDIDK
                     PLLGHGRVQWDNVDASALAAITPALEGLGGKFRGTIAMGPARDPRPLEPLRIDVNIGA
                     DDGHFRGVRIGSPRDIVAMHAVAYANLDRAVLDHCDLNVADGVVHLWMRAGKGLVSQA
                     AQISFENLQLDELAHIDPDRTTPMPGLVSGNFAFVGSGLNTDRLLGKGKIDLTQTDLG
                     NWGPIAALYNVMNGGGSQQPSGVGSLALRFEQNTLRVTDFRFFNRGIDAFGVFTVGPS
                     VWDLQHAPVSGQVVGSIRTLKNTRIPLLADFDEIFSIVQGSLTAIDIGGTKQNPTYVQ
                     AGVAAIGNDLRTLIVGSARSENGAQ"
BASE COUNT          280 a          514 c          535 g          306 t
ORIGIN      
        1 ccgaccgtct cccggagacc tgcggtcagc agtaccgggc gagggggacg gaccagaacc
       61 gggcgacaaa ttaaagtcat acccacgccg ggttcggagg tactggcgac gacccggatg
      121 ctttgcttca acccagcaga agaatctcgc ttcgtcgcta gtacgctccg aacccggcgt
      181 gggggcttcg ttgggaccag aatggttact gcgcgccgtt ttcggagcgg gcgctgccga
      241 cgatcagcgt gcggagatca ttgccgatcg ccgcgacgcc ggcttgtacg tacgtgggat
      301 tttgtttggt gccgccgatg tcgatggcgg tcaacgatcc ctgcacgatg ctgaagatct
      361 cgtcgaagtc cgccagcagt ggaatgcgcg tgttcttcaa cgttcggatc gaaccgacca
      421 cctgaccact gaccggcgcg tgctgcaggt cccacacgct ggggccgacg gtgaagacgc
      481 cgaatgcgtc gatgccgcgg ttgaagaagc ggaaatcagt gacgcgcagc gtgttttgtt
      541 cgaagcgcag ggcgagcgaa ccgacaccgc tgggctgctg gctgccgccg ccgttcatca
      601 cgttgtacaa cgcggcgatc ggtccccagt tgcccaggtc cgtctgcgtc aaatcaatct
      661 tccctttgcc gagcaggcga tcggtgttca acccgctgcc gacaaatgcg aagttgcccg
      721 agaccaaacc cggcatgggc gtggtgcgat cgggatcgat gtgggccagc tcatcgagtt
      781 gaagattttc aaagctgatc tgcgcggcct gcgacaccag ccccttgccc gcgcgcatcc
      841 agaggtggac gacgccatcg gctacgttga gatcgcaatg gtccagcacc gcgcggtcga
      901 ggttggcgta tgcgacggcg tgcatggcga cgatgtcgcg cgggctgccg atgcgcacgc
      961 cgcggaaatg gccgtcatcg gcgccgatgt tcacgtcgat gcgcagcggc tcgagcgggc
     1021 gcgggtcgcg cgcgggaccc atcgcgatcg tcccgcggaa tttcccgcca aggccttcca
     1081 gcgccggggt gatcgccgcc agcgcgctgg cgtccacgtt gtcccactgc acgcgcccgt
     1141 ggcccagcag tggtttgtcg atatcgacgg tggccgcccc atcgaacgtg ccgctgagca
     1201 ggttgccggc gatcttctgc acggccaaca tccggccttg caggttcgca accagatcca
     1261 cgtgcgatag cggggcgttg tggtactgca gatcgaccgt cgccgacagc ggaccactgg
     1321 ctcctttgtg ctcgagatcg atatcgagct tcgtcgccgc cgtcagcgcg gccgtcgcgc
     1381 caccggtggt gccgtacggc cacttgtcga tcttcacatc cgtaaaaatg tggcgggggt
     1441 tggacagctc gtacgagatg gcggcgtcga gtcgcccggc gttgtacgtg gccgccaccg
     1501 gcgcgagctt gagcacgccc gcgtccagcg atgctttgcc ttcgatgcga tcgattttca
     1561 aaccgcgagc atcgaggttc tgcacgacgt aatcgccgtt gaccgtgatg tgattcaaac
     1621 cgccgctctt gggca
//
LOCUS       DATIMH010001150         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2012444, whole genome shotgun sequence.
ACCESSION   DATIMH010001150 DATIMH010000000
VERSION     DATIMH010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012444"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..845
                     /gene="larE"
                     /locus_tag="VLI90_19835"
     CDS             30..845
                     /gene="larE"
                     /locus_tag="VLI90_19835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HSV16524.1"
                     /translation="MSALKEQRLRELVSSFDSLIIAFSGGVDSAYLAWVATAVLGPGA
                     LCVTADSPSYPDRHRRLALRVAKQFGFRHEIVHTRELERAEYRSNPTNRCYYCKHELY
                     SLLSEVARDREFDVIADGSNADDRGDYRPGRQAAREFGVRSPLDDVGLTKAEIRELSH
                     LAGLPTWDEPASACLSSRIPYHSEVTDEKLRMIERAEDVLHDLGFRVCRVRHHEQLAR
                     LEIGLDELPRALEPAVRDRLLTELKAIGYTHITIDLQGYRMGSLNEGLRLRPV"
     gene            817..>2305
                     /locus_tag="VLI90_19840"
     CDS             817..>2305
                     /locus_tag="VLI90_19840"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HSV16525.1"
                     /translation="MKASDSGRSDRLFTRAAYVAAVVWLLAHLPSLAPALEDIDSINF
                     ALGLHHFDPSQHQPHPPGYPVYIAVGRIALAVLHVVASGLSYVRSDALALAIWSTVGG
                     AIAIVAAAYVFRELASDQERASAVGFWGAVLMAVTPLFWITGLRPMSDMFGLGVALAA
                     QLFLLRGGRDGRSLVAGAAIAGLAAGIRTQTVPLTVPLLLYALFAQRQRGTWLVRPIA
                     AFVAAVLTWAGPLLVLTGGVNGYLRALGSQAGEDFAWVDMLWAHPTPRAIAFAVYDSF
                     LLPFANPITGGIVLAIATLGVLRMLVVDRRAVLLLAIAFVPYAIFHLLFQEAANLRYA
                     TPLVVPIAFAAAFLACSLEGPFARRVNRQVFVAAPLIVLLVMPNLIVGPVLASTAGIP
                     IVAGYVYGREPHPAFRAIDDMTREARGHKPAAVFSHFSLRRPLQAAPTPLPVVEPKRN
                     IEWLAVEDYWLRGGTQQVWFLADPRRTDLALIDPQVRQMGERTEYR"
BASE COUNT          321 a          781 c          781 g          422 t
ORIGIN      
        1 aggaactcga cgattaaggt gccggtccga tgagtgcgct caaggagcag cggctccgtg
       61 agctggtctc gtccttcgat tctctgatca tcgcgtttag cggcggcgtc gacagcgcct
      121 acctggcgtg ggtggccacc gcggtacttg gtcctggcgc gctctgcgtg accgcggaca
      181 gccccagcta cccggatcgt catcgtcgcc tcgcgcttcg cgtcgccaag cagttcggct
      241 tccggcacga gatcgtgcac acgcgtgagc tcgaacgcgc tgagtaccgt tccaacccga
      301 ccaaccggtg ctactactgc aagcacgagc tgtactcgct gctcagcgag gtggcgcgcg
      361 atcgcgagtt cgacgtgatc gccgacggca gcaacgccga cgatcgaggc gactaccgcc
      421 ccggccgtca ggccgcgcgg gagttcgggg tgcgcagccc gctcgacgac gtgggcctca
      481 ccaaggcgga gatccgcgag ctgtcgcatc tcgctgggct gccaacgtgg gacgagccgg
      541 catcggcgtg tctctcatcg cgtatcccct atcattccga agtcaccgac gagaagctcc
      601 ggatgatcga acgcgcggag gacgtgctgc acgatctcgg ctttcgcgtc tgccgcgtga
      661 ggcatcacga gcagttggcg cggctggaaa tcggactcga cgaactgccg cgcgcgctcg
      721 aacccgccgt ccgggatcgg ctgctcaccg agctgaaggc gattgggtat acgcacatca
      781 ccatcgatct gcagggctac cgcatgggca gcctgaatga aggcctccga ctccggccgg
      841 tctgaccggc tgtttacgcg cgcggcgtac gtcgcagcgg tagtctggct gctggcgcac
      901 ctgccgtcgc tcgcgccagc gctcgaagac atcgactcga tcaacttcgc gctcgggctg
      961 catcacttcg atccgtcgca gcatcagccg catccacctg gctacccggt ctacatcgcc
     1021 gtcggacgca tcgcgctcgc ggtgctgcac gtggtcgcat cgggactctc gtacgtccga
     1081 agcgacgcgc tggcgctggc gatctggtcc accgtcggcg gcgcgatcgc gatcgtcgct
     1141 gccgcgtacg tctttcgaga gctggcgtcg gaccaggaac gcgcgtccgc cgtggggttc
     1201 tggggcgcgg tgctgatggc cgtcacgccg ctcttctgga tcaccgggct gcgtccgatg
     1261 agcgacatgt tcgggctggg cgtggcgctg gcggcccagc tattcctgct gcgaggcggc
     1321 cgcgacggtc gcagcctggt ggccggcgcc gcgatcgccg ggctcgccgc agggatccgc
     1381 acgcagacgg ttccgctgac ggtcccgttg ctgctctacg cgctgttcgc gcagcgccag
     1441 cgtgggacgt ggctggtgcg tcccatcgcc gccttcgtgg ctgcggtcct gacatgggcg
     1501 gggccgctgc tcgtcctgac cggcggcgtg aacggatacc tgcgtgcgct gggcagccag
     1561 gcgggcgagg acttcgcgtg ggtggacatg ctgtgggcgc acccaacgcc gcgggcaatc
     1621 gcgttcgccg tctacgacag cttcctgctg ccgtttgcga acccgatcac cggtggcatc
     1681 gtgctcgcga tcgcgacgct gggggtcctt cggatgctgg tcgtggatcg ccgtgccgtg
     1741 ctgctcctgg cgatcgcttt tgtgccgtac gcgatctttc acctgctgtt ccaggaagcg
     1801 gccaacctgc ggtacgcgac gccgctcgtg gtgcccatcg ccttcgccgc cgcgttcctg
     1861 gcgtgttcgc tggaagggcc attcgcgcgc cgggtgaatc ggcaggtctt cgtcgccgcg
     1921 ccactcatcg tgctcctggt gatgccgaat ctcatcgtcg gcccggtgct ggcgagcacc
     1981 gccggcattc ccatcgtggc tggctacgta tacggccgcg agcctcaccc agcgtttcgc
     2041 gccatcgacg acatgaccag ggaggccagg ggccataagc cggcggcggt gttctcgcac
     2101 ttctcgctgc gccgcccgct gcaggccgcg ccgacgccgc tgcccgtcgt cgagccgaag
     2161 cggaacatcg aatggctcgc cgtcgaggat tactggctga ggggtggtac gcagcaggtg
     2221 tggttcctcg ccgatccccg acggactgat ctggcgctca tcgatccgca ggtccgtcag
     2281 atgggagagc ggaccgaata tcgct
//
LOCUS       DATIMH010001151         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_271527, whole genome shotgun sequence.
ACCESSION   DATIMH010001151 DATIMH010000000
VERSION     DATIMH010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271527"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2148)
                     /locus_tag="VLI90_19845"
     CDS             complement(<1..2148)
                     /locus_tag="VLI90_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16526.1"
                     /translation="MGAPGAPTAPAAPVVTIPENEPLKDSVENFWHYGKIARYDIASA
                     EGQHILSLNADPQQLLSTFESVAQAHHDNLDQWLLRWQGVDPMKDVTTQLANALGKGH
                     NARRNDPKYIEENIKTLATNERGYGIAIGRLSDSGELAVPMMIDYLRDPSKSQYQPAI
                     RRALRDLGLKALNPLLAATEMKDENALMSIVSAIGDIGYVEVSVPYLARLLQLPDVPA
                     SVKAATNDALQRMQQNPKSLSATDLFYDLSERFYYDNAAIRADKRMPVGYVWYWSDTK
                     GLNKIDVPQPIFHDVMSKRAAEYSMKLGQSQGDALSLWIAASFQDEADLPAGQKDPTL
                     PADAPSAHYYGVAAGTKYLNNTLARANKDRSSAVALKAIQALEEIGGQANLFSGDQHP
                     LTDSLQFPDRLVRYESAFAIAGALPRQPFPGQERIVPLLSEALSQTGAANVLVVTPDQ
                     DQLNGMLDGLKGAGYNVVGGTSPESAVAAAAARPAIDAIVINEELGPTKIDQLIGLAA
                     QTPRLERAVKVVITRTKASPYATQAVNDTMMSTTQAKPGDTAALKTAIDDARKRGGLL
                     PLDEAAASKYATRAADLLVNIAISHSPVFDLTVAQSLLLSALDDPRPEIVKADGTVLG
                     QINSNEVQPALLIKAADDKTADDLKIATFKALATNARNFGNHLEAPQVETLQKVVAAA
                     MNLDVRTAASEARGALDLPADQAKQLIVQQSKTT"
BASE COUNT          316 a          752 c          760 g          443 t
ORIGIN      
        1 cgtcgtcttc gactgctgca cgatcagctg cttcgcctga tcggccggca ggtcgagcgc
       61 cccgcgcgct tcgctggcgg cggtgcggac gtccaggttc atggcggcgg cgaccacctt
      121 ttgcagcgtc tccacttgcg gcgcctccag gtgattcccg aagttccgcg cgttcgtcgc
      181 cagcgctttg aacgtcgcga tcttcaggtc gtccgccgtc ttgtcgtccg ccgccttgat
      241 cagcaacgcc ggctggactt cattgctgtt gatctggccc agcaccgtgc cgtccgcctt
      301 gacgatctcc ggccgcgggt cgtccagcgc cgacagcagc aggctttggg cgaccgtcag
      361 gtcgaacacc gggctgtgac tgatcgcgat gttcacgagc aaatcggcgg cgcgcgtcgc
      421 atatttggac gcggcggctt cgtccagcgg gaggagcccg ccgcgcttgc gggcgtcgtc
      481 gatcgccgtc ttcagcgcgg cggtgtcgcc gggcttggcc tgcgttgtgg acatcatcgt
      541 gtcgttcacc gcctgcgtgg catacggcga cgcctttgtg cgcgtgatga ccaccttcac
      601 cgcccgctcc aaccgcggcg tctgggccgc caggccgatc agctggtcga tcttcgtcgg
      661 cccgagttct tcgttgatga cgatcgcgtc gatcgccggt cgagccgcgg cggcggcgac
      721 ggcggattcg gggctcgtcc cgccgaccac gttgtagccc gcgcccttca acccatcgag
      781 catgccgttg agttgatcct gatccggcgt gacgaccagc acgttggccg cgccggtctg
      841 gctcagcgct tcgctgagca gcgggacgat gcgctcctgc ccggggaagg gctggcgcgg
      901 cagcgcaccg gcaatcgcga aggctgattc gtatcgcacc aggcgatcgg ggaactgcag
      961 cgaatcggtt agcggatgct gatcgccgga aaacaggttc gcctgtccgc cgatttcttc
     1021 cagcgcctgg atcgccttca gcgcgacggc ggagctgcgg tccttgttcg cccgggccaa
     1081 tgtgttgttg aggtacttcg tccccgcggc aacgccgtaa taatgcgccg acggcgcatc
     1141 cgctggcagc gttgggtcct tctggccagc cggcaggtcc gcttcgtcct ggaagctggc
     1201 ggcaatccac aagctcagcg catcgccctg cgattgcccg agcttcatcg aatactcggc
     1261 cgcgcgcttg gacatcacat cgtggaagat cggctgcggc acgtcgatct tgttcaggcc
     1321 cttggtgtcg gaccagtacc agacataacc gaccggcatg cgcttgtcgg cgcgaatggc
     1381 ggcgttgtcg taatagaacc gctcggacaa atcgtagaac agatcggtag cgctgagcga
     1441 tttcgggttc tgctgcatgc gctgcagcgc gtcgttggtg gccgccttca cgctggccgg
     1501 cacgtccggc agctgcagca ggcgcgccag gtacggcacc gaaacttcga cgtaaccgat
     1561 gtcaccgatc gccgacacga tcgacatcag cgcgttctcg tccttcatct ccgttgccgc
     1621 cagcagcggg ttcaaggcct tcaggcccaa gtcgcgcagc gcccggcgga tggccggctg
     1681 atattgcgac ttcgacggat cgcgcaggta gtcgatcatc atcggcacgg ccagctcgcc
     1741 gctgtcgctc agccggccga tcgcgatgcc ataaccgcgc tcgttggtcg ccagcgtctt
     1801 gatgttctct tcgatgtact tcgggtcgtt ccgccgggcg ttgtgaccct tgcccagcgc
     1861 gttggccagc tgggtggtca cgtccttcat cggatcgacg ccctgccagc ggagcagcca
     1921 ctggtcgagg ttgtcatgat gcgcctgcgc gaccgactcg aacgtcgaga gcagttgttg
     1981 cggatcggcg ttcagcgaca ggatgtgctg gccctcagcg ctggcgatgt cgtatcgggc
     2041 gatcttgccg taatgccaga agttctcgac gctgtccttg agcggctcgt tctcggggat
     2101 ggtgaccacc ggcgccgcgg gggctgtcgg agcgccaggt gcgcccacgg cgggtgcgcc
     2161 ggcagcgccc gtatcagtcg gtgcgccggc agcgggtgcg ccggcagcgg atggcgcgga
     2221 tgtcgccggc gcgccggccg cgccacccgc gggcggggtt tcctgcgcga a
//
LOCUS       DATIMH010001152         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3162248, whole genome shotgun sequence.
ACCESSION   DATIMH010001152 DATIMH010000000
VERSION     DATIMH010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3162248"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /gene="kdpB"
                     /locus_tag="VLI90_19850"
     CDS             complement(<1..1980)
                     /gene="kdpB"
                     /locus_tag="VLI90_19850"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HSV16527.1"
                     /translation="MKVQPMITHGKSQRLFDPSIVRRAALDAVLKLDPRHQVKNPVMF
                     VVFIGSVITSALFVQALIGHGEAPAGFILAVSVWLWFTVLFANFAEAMAEGRGKAQAD
                     TLRRARRDTPAKRLAEPRFGSTFKTVSAATLVKNDVVLVEAGDYVPVDGEAIEGMASV
                     DESAITGESAPVIRESGGDRSSVTGGTRVLSDWLVVRVGVNPGETFLDRMIAMVEGAK
                     RQKTPNEIALNILLAAMTIVFLLATVTLLPFSIFSVQQIGRGDPVTVTVLCALLVCLI
                     PTTIGGLLSAIGIAGMDRMIQANVIAMSGRAVEAAGDVDVLLLDKTGTITLGNRQAVA
                     FHPSNGTPDRELADAAQLSSLADETPEGRSVVVLAKEKYGIRERQLHELNATFVPFTA
                     QTRMSGVNFDHREVRKGAAAAIEAHVKGAGGAFPAQVTQTVERISKGGGTPLVVADGA
                     RVLGTIELKDIVKGGIKERFAELRRIGIKTVMITGDNPLTAAAIAAEAGVDDFLAQAT
                     PEAKLKLIRDYQAGGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDS
                     NPTKLIEIVETGKQLLMTRGALTTFSIANDVSKYFAIIPAAFATTYPVLNRLNVMHLA
                     TPASAILSAVIFNAVIIIFLIPLALRGVRYRPVPAQ"
     gene            complement(2094..>2428)
                     /locus_tag="VLI90_19855"
     CDS             complement(2094..>2428)
                     /locus_tag="VLI90_19855"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HSV16528.1"
                     /translation="GPHGFSEILYAMSSTGNNNGSAFAGLSANNPFYNIAGGVLMLIS
                     RYWLIVPALAIAGSLGAKKHVPASSGTLPTHTPLFVATIVGIVVLVGALSFIPALALG
                     PIVEHLMR"
BASE COUNT          394 a          787 c          805 g          442 t
ORIGIN      
        1 ctgcgccggc accggccgat agcgcacgcc gcgcagggcg agcgggatca ggaagatgat
       61 gatgactgcg ttgaagatga cggcggacag gatcgcgctg gccggcgtgg ccaggtgcat
      121 cacgttcagg cgattcagca ccggataggt cgtcgcgaac gccgcgggga tgatggcgaa
      181 atatttgctg acgtcgttgg cgatgctgaa cgtggtcagc gcgccgcgcg tcatgagcag
      241 ctgcttgccg gtctcgacga tctcgatcag cttcgtcggg ttcgagtcca gatccaccat
      301 gttgcccgct tccttggccg cctgcgtgcc ggtgttcatc gcgacggcga catccgcctg
      361 cgccagcgcc ggcgcgtcgt tggtgccgtc gccggtcatc gcaacgagcc ggccgccggc
      421 ttgatagtcg cggatcaact tcagctttgc ctccggcgtg gcctgcgcca ggaagtcatc
      481 gacacccgct tcggcggcaa tcgccgcggc agtcaacgga ttgtcgccgg tgatcatgac
      541 ggtcttgatg ccgatgcggc gcagctcggc gaagcgctcc ttgattccgc ccttgacgat
      601 gtccttaagc tcgatggtgc cgagcacgcg ggcgccgtcg gcaacgacca gcggcgtgcc
      661 gccgcctttg ctgatgcgct cgaccgtttg cgtgacctgc gcggggaacg ccccgccggc
      721 gccctttacg tgcgcttcga tggcggcggc cgcgcctttg cggacttcgc gatggtcgaa
      781 gttgacgccg ctcatgcgcg tctgcgcggt gaagggaacg aacgtcgcgt tcagctcatg
      841 cagttgccgc tcgcgaatgc cgtatttctc cttggccagc acgacaacgc tgcggccctc
      901 gggcgtttca tcggccagcg acgacagctg cgccgcgtcg gccagctcgc gatccggcgt
      961 gccgttgctg ggatgaaacg ctaccgcctg gcgattgccc agcgtaatcg tgccggtctt
     1021 gtccagcagc agcacgtcca catcgccggc ggcctcgacg gcccggccgc tcattgcgat
     1081 gacgttcgcc tgaatcatgc gatccatccc ggcaatgccg atcgcgctga gcagcccgcc
     1141 gatcgtcgtc gggatcaggc agaccagcag cgcgcacagc acggtcaccg tgacgggatc
     1201 gccgcggccg atctgttgca cgctgaagat gctgaacggc agcagcgtga cggtggccag
     1261 caggaagacg atcgtcatcg cggccagcag gatattgagc gcaatttcat tcggcgtctt
     1321 ctggcgcttg gcgccttcga ccatggcgat catgcggtcg aggaacgttt cgccagggtt
     1381 tacgccgacg cgcacgacca gccaatcgct gagcacgcgg gtgccgccgg tgacgctgga
     1441 gcgatcgccg ccgctttcgc ggatgacggg cgcggattca ccggtgatgg ccgattcatc
     1501 caccgacgcc atgccttcga tcgcttcgcc atcgaccggt acatagtcgc cggcctcgac
     1561 gaggacgacg tcgttcttga cgagcgtggc ggccgagacg gtcttgaagg tcgagccgaa
     1621 ccgcggctcc gccaggcgtt tcgccggtgt gtcgcggcgc gcccggcgaa gcgtgtccgc
     1681 ctgcgccttg ccgcgtccct ccgccatcgc ctcggcgaaa ttggcgaaca acacggtgaa
     1741 ccagagccaa accgagacgg caaggatgaa ccccgccggc gcttcgccgt ggccgatgag
     1801 cgcttgaacg aacagcgcgc tggtgatgac gctcccgatg aagacgacga acatgaccgg
     1861 gttcttcacc tggtggcgag ggtcgagttt gaggaccgcg tctagcgccg cgcggcggac
     1921 gatcgatgga tcgaacagcc gttgagattt gccgtgtgta atcattggtt gcaccttcat
     1981 cgtgacacgc gcccttcggc ccgtgctggg aactttggga tttcggacag atcaagcttt
     2041 ctttagtcga gcgcttcacg cacgggcccg aggcccgtgt cacgattgag tcgtcatcgc
     2101 attagatgct ccacgatcgg tcccagcgcc agcgcgggga tgaagctgag cgccccgacg
     2161 agcacgacga tgcccacgat cgttgcgacg aacagcggcg tgtgcgtggg cagcgtgccg
     2221 gagctggccg gcacgtgctt cttcgccccc aatgaaccgg cgatcgccag cgcgggcacg
     2281 atcagccaat accggctgat cagcatcagc acgccgccgg cgatgttgta gaacgggttg
     2341 ttggcgctca gccccgcgaa cgcgctgccg ttgttgttcc cggtgctgct catcgcatac
     2401 aggatctcgc tgaacccgtg tgggccgg
//
LOCUS       DATIMH010001153         7536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4605200, whole genome shotgun sequence.
ACCESSION   DATIMH010001153 DATIMH010000000
VERSION     DATIMH010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4605200"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="VLI90_19860"
     CDS             complement(<1..1864)
                     /locus_tag="VLI90_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16529.1"
                     /translation="MKQPSLNPLRLFNSVIFGISIMVLVAAYIAIGSGCASVREYFEM
                     DELQFFNAWPLKALMALLVINLVVVTWNRIPLTPPRYGVWCVHAGIVTLILATSFYYR
                     NKIEGTTRLYTSPEFGPVSTDHFYDKDQRALYLKIDGTMWNWYALPSLPRFKAYDATL
                     GNAAALDRDDLRKIEPYIEVQDDKSKPPIRRSLSQTLGWKDELRLAVVGYYPFANVNT
                     SFAQDPAGDLAGLRVSLPDMHEGENFETWLVGADARHRFTSLEGTDLEHRWVDAATLS
                     ILKQSIDKIFRLDVTVGGATQTIYVQPGRSYPVGQSGYKIDVENFNPAWPMFGTREIV
                     QALTLKVTTPTQTFRRMVLHGRDLQTDFKLGDNDPSVGPMGKRQKEPLDKDLLIRFKF
                     TDPAGLLPRDGTIKHTLLTGGDERGITDIVAGLSTPGEVRTFDNGVGDVALTPADPMA
                     NAPFAKMAAAADQPPADQQPPHPAIKLHLERVDHLRRDDAVQIVPPARRTRDAGESGQ
                     FQVLRVRVTLGDWSRDVVVPYSLDAGDISWDGGSIKLPTGSTLQLALGQVKLPLPARL
                     TLAKFEVVPYPGGDTSTRSMMLDFRSTLKVEDIDSGESATEIAHMNSPVYFGDGS"
     gene            2000..2596
                     /locus_tag="VLI90_19865"
     CDS             2000..2596
                     /locus_tag="VLI90_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16530.1"
                     /translation="MITVRQMERLWSGKAYEKLFRELLAARPEASLRLEMELGRSIPT
                     AALAIVRLDELSQAYVPLYSQLVRTVLNDQHSDGGWGDPMITAICVRALTCGGGNGVA
                     VERGLQYLANMQKAEGIWPNVPIRRMPADPYVSAFILLQLGDHAAFRRAVRFFDAINW
                     FEANEAGLDPDARRLWDRASLRCGMKSATRNGSGPLWS"
     gene            2979..3689
                     /locus_tag="VLI90_19870"
     CDS             2979..3689
                     /locus_tag="VLI90_19870"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSV16531.1"
                     /translation="MIMATPETFASDTPTPQTNDTGARRLFSQSPLDVPRRIVAAGEA
                     IHPAHAPADHVYFIDRGEVRLYQPGPDGTARLVEILGPGDWFGVAALAGSVTCEKSAI
                     AITNTAIAQIPLDQFLAVLERDPQSSVLFLRDLARRLHAARTATSRFVFDDCERRILK
                     TLVDFSRTAAASPTTGNEVALRITHQQLAQAIGVARETVSLALTKLRRQNLVQTGRNR
                     LVFNPLMLQEFMSRGAAQ"
     gene            complement(3707..4462)
                     /locus_tag="VLI90_19875"
     CDS             complement(3707..4462)
                     /locus_tag="VLI90_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16532.1"
                     /translation="MNTLLLTATITPPPGVPGLRRTDPSERLRDYAEALSFYCALPAD
                     VIHRIVFIENSATDVTPLRDVAAKAEASQRVEFVSFNGLDHPAAYGRGYGEFKLLDYA
                     MQHSPSLVAANEDERLWKATGRYRILNLVDLIRTAPKTFDLYCDLRDRPIPWMDLRVF
                     AATVGGYRRLLMGIYEQLREDVIHVSPERYLRPVIGELAKSHQIVTRFCREPRIDGIR
                     GMDSRNYTSGVNLLKYWYRATTRRLQGLVNPPA"
     gene            4657..5721
                     /locus_tag="VLI90_19880"
     CDS             4657..5721
                     /locus_tag="VLI90_19880"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HSV16533.1"
                     /translation="MIQLFSPLKLRDITFRNRIFVSPMCQYSSDDGMPNDWHLVHLGS
                     RAVGGAACVIVEATGVSREGRISPFDSGIWSDAHAAGFRRITAFIKQHGAAPGVQLAH
                     AGRKASTDAPWTGGKPVPPSSGGWQPIAPSPIPFSDGYPTPRQMTRGDIDAVTKEFVA
                     ATRRSLDAGFELVEIHMAHGYLLHSFLSPLSNHRRDDFGGSLANRMRFPLQVTRAMRN
                     VWPAHLPLLVRISASDWADGGWDLPQSIAFARELKKLNVDLIDCSSGGAVPYAKIEAG
                     PGYQVPFASAIRRDAGIATGAVGMITDAQQAESIIANGEADIVLLARAMLKDPYWPMH
                     AADQLGMKIEYWPKQYGRAR"
     gene            5850..6512
                     /gene="msrA"
                     /locus_tag="VLI90_19885"
     CDS             5850..6512
                     /gene="msrA"
                     /locus_tag="VLI90_19885"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSV16534.1"
                     /translation="MALSTFFGAPKAGAAAAGPLPAPAVDLPADKDAKPGEQHTTVVA
                     GGCFWCIEGVFRQLNGVTNAVSGYAGGSKETANYETVCTGATGHAEAVKITYDPTKIT
                     YGQLLRALFTVIDPTTKNRQGPDSGTQYRSAIFYENDDQRRVAQAYIDQLNAAKVFPY
                     PIVTTLEPLKPEAFYPAEAYHQNYTACHLNNPYIIQEALPKVEKIRAAMPDAVKPATT
                     QP"
     gene            6618..>7536
                     /locus_tag="VLI90_19890"
     CDS             6618..>7536
                     /locus_tag="VLI90_19890"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV16535.1"
                     /translation="MLPIFSAAYAPADVTGGKTRCARMTNTRTKSVPPLRAAAIGTGK
                     ISAEHLRYLSGAGARAGVSKLVGVCDLSPSMANFAAEEFGADGAFTDYAKMLADAKPD
                     VVHILTPPHTHARLVADCLDRGAHVIVEKPVAPTHDEFLSLWERSQRTGRRLIEDHNY
                     RFNEPILAIEKLLADGKLGDVREVEVRMALAVRDKGSRYMDQSLPHPSHRLPCGIIHE
                     FITHLCYLALRFMPKDAAFDRVAAAWSKHGSDALFKFDDLDATVITGDVHARLRFTSH
                     AAPDCFAVTVRGSKGYAETDLFQPHLRVAM"
BASE COUNT         1375 a         2472 c         2340 g         1349 t
ORIGIN      
        1 acgaaccgtc cccgaagtag acggggctgt tcatgtgcgc gatctcggtg gccgattcgc
       61 cggagtcgat gtcttcgact tttaacgtcg agcggaagtc gagcatcatg ctgcgcgtgc
      121 tggtgtcgcc gccggggtac gggaccactt cgaactttgc cagcgtgaga cgcgccggca
      181 acggcagctt cacctgcccg agcgccagct gcagcgtgct gccggtcggc agcttgatcg
      241 acccgccatc ccatgagata tcgccggcgt ccagcgagta aggaacgacc acatcgcgcg
      301 accagtcgcc cagcgtgacg cgcacgcgca agacctggaa ctgtccagat tcaccggcgt
      361 cgcgcgtgcg ccgcgccggt ggcacgatct gcacggcgtc gtcacgccga aggtgatcga
      421 cgcgctccag gtgcagcttg atggccgggt gcggtggttg ttggtccgcc ggcggttggt
      481 ccgcggcggc ggccatcttc gcgaacggcg cgttcgccat gggatccgct ggggtgagtg
      541 cgacatcacc cacgccgttg tcgaacgtcc gcacctcgcc cggggtcgac agccccgcga
      601 cgatgtcggt gatgccccgc tcatccccgc cggttagcag cgtgtgcttg attgtcccgt
      661 cgcgcggcag cagccccgcg ggatccgtga acttaaagcg aatgagcagg tccttatcga
      721 gcggctcctt ctgccgcttg cccatcgggc cgacgctggg gtcattgtcg ccaagcttga
      781 agtcggtctg caagtcgcgc ccgtgcagga ccatgcgacg aaacgtttgg gtcggcgtcg
      841 tcaccttcag cgtcagcgcc tgcacgatct cgcgcgtgcc gaacatcggc cacgcggggt
      901 tgaagttttc cacgtcgatc ttgtacccgc tctggccgac gggatagctt cggccaggtt
      961 ggacgtagat cgtctgcgtc gcgccgccga ccgtcacgtc gaggcggaag attttgtcga
     1021 tggactgttt caggatcgac agcgtcgccg catcgaccca gcgatgttcc agatcggtgc
     1081 cttcaaggct ggtgaagcga tggcgggcgt cggcgccgac cagccaggtt tcgaaattct
     1141 ccccctcgtg catgtcgggc aggctcacgc gcaagccggc cagatcaccc gcggggtctt
     1201 gcgcgaagct ggtgttgacg ttggcaaagg ggtaatagcc gacgacggcc aggcgaagct
     1261 catccttcca cccgagcgtc tgagaaaggc tccgccgaat gggcggcttg gacttgtcat
     1321 cctgcacttc tatatatggc tcgatcttcc gcaggtcatc gcgatcgagc gccgcggcat
     1381 tccccagcgt cgcgtcatac gccttgaacc gcggcagcga cggcagcgcg taccagttcc
     1441 acatcgtgcc gtcgatcttc aggtacaggg cgcgctggtc cttgtcgtaa aaatggtcgg
     1501 tggagacggg gccgaattcg gggctggtgt acaggcgggt cgtgccttcg atcttgttcc
     1561 ggtaatagaa cgacgtcgcc aggatcagcg tgacgatccc cgcgtgcacg caccacacac
     1621 cataccgcgg cggcgtgagc gggatgcgat tccacgtcac cacaaccagg ttgatgacga
     1681 gcaacgccat cagcgccttc agcggccagg cgttgaagaa ctgcagctcg tccatctcga
     1741 aatattcgcg aaccgacgcg cagcccgaac cgatcgcgat atacgccgcc accagcacca
     1801 tgatgctgat gccgaagatg acgctgttga aaaggcgaag cgggttgagg gacggctgtt
     1861 tcacgcgaat tttggcactt cgtgacggat tgtacggtct tggggattca attctagttt
     1921 actgaaaatc ttcttgacag tccggtaact gttagggcat attatcgccc taacaagaac
     1981 ctatcagcaa aggattgtca tgatcacggt tcggcagatg gaacggctct ggagcggcaa
     2041 ggcgtacgaa aagctctttc gcgaactgct ggcggcaagg cccgaggcgt cgctgcggtt
     2101 ggagatggag ctgggccgat cgatccccac ggcggcgctg gcgatcgttc ggctggacga
     2161 actgagccag gcgtatgtgc cgctctattc gcagcttgtc cgaactgtgt taaatgacca
     2221 acacagcgac ggcggctggg gcgacccgat gatcaccgcc atctgcgttc gcgccctgac
     2281 ctgcggtggc ggcaacggtg tggccgtcga acgcgggctt caatatctgg ccaacatgca
     2341 gaaggccgaa ggcatctggc cgaacgtgcc gatccgccga atgccggccg atccctatgt
     2401 ctccgccttc atcctgctgc aactgggcga ccacgccgcg ttccgccggg cggtcaggtt
     2461 tttcgatgcg atcaactggt tcgaagccaa tgaggcaggt ctcgatcccg atgcccgccg
     2521 gctgtgggac cgcgccagcc ttcgctgcgg aatgaagtcc gcgacacgaa acggcagcgg
     2581 gccgctctgg tcataatcgc gccagaccag ccccgccgtc acacgattca caaccgcact
     2641 cgacccccgg caaaagtgga gccaccgccg ggggttgcgt gcgcgccgag caggcgccga
     2701 cagatccctg agccccttta gtccaccccc acaccgggtt cggaggagcc tgccctgagc
     2761 ctgtcgaagg gtacccgcgg cgacccggat tctgcggcgc ggtaggggaa gagatccggg
     2821 tcgtcggagt acctccgaac ccggcgtgag ttaaaccagc ccaaatccca ggtttccagt
     2881 atcatccttt tgctaccgaa cccaccgcgc gcagcgcacg tacgtgggga aaaggcgtgc
     2941 ccgctatagt gcggcacccc cgttctttgg agatttggat gattatggcc acgccggaga
     3001 cgttcgcgtc cgacaccccg accccccaaa ccaacgacac cggggctcgc cggttgttct
     3061 cgcaatcgcc gctggacgtg ccgcgccgca tcgtcgccgc cggcgaagcc attcatcccg
     3121 cccacgcccc cgccgatcac gtttacttca tcgaccgcgg cgaagtccgc ctgtatcagc
     3181 ccggtcccga cggcaccgcg cgcctggtcg agatcctcgg ccccggtgat tggttcggcg
     3241 tcgccgcgct ggccggttcg gtcacgtgtg aaaaaagcgc catcgccatc accaacaccg
     3301 ccatcgcgca gatcccgctt gatcagttcc tcgccgtgct cgagcgcgat ccgcaatcga
     3361 gcgtcctgtt cctgcgagac ctcgccagac gcttgcacgc ggcgcgaacc gcaacgtcgc
     3421 gcttcgtctt cgacgactgc gaacggcgga tactcaagac gctggtcgac ttcagccgca
     3481 ccgccgccgc ctcgcccacc accggcaacg aggtcgcgct tcggatcacg catcagcaat
     3541 tggcccaggc gatcggcgtc gcgcgcgaaa ccgtcagcct cgcgctgacg aaactccgcc
     3601 ggcagaacct cgtgcagacc ggccgcaacc ggctggtgtt caatccgctg atgcttcagg
     3661 aattcatgtc gcgcggcgcc gcgcagtaat gcgggcgggc gcgcgctcac gccggtggat
     3721 tgaccaggcc ttgcaatcgc cgggtcgtcg cgcggtacca atacttcagc aggttcacgc
     3781 cgctggtgta gttccgcgaa tccatcccgc gaatgccgtc gatccgcggc tcccgacaga
     3841 agcgcgtgac gatctgatgt gacttggcca gctcgccgat caccggccgc aggtaacgct
     3901 cgggcgagac gtgaatcacg tcctcgcgca gctgctcgta gatccccatc aacagccggc
     3961 gatatccccc caccgtcgcc gcgaacacgc gcaggtccat ccacggaatc ggccgatcgc
     4021 gcaggtcgca atacaaatcg aatgtcttcg gcgccgtgcg aatcagatcg accagattca
     4081 agatccgata ccgccccgtc gccttccaca atcgctcatc ctcattcgcc gccaccaacg
     4141 acggcgagtg ctgcatcgca taatcgagca gcttgaattc cccatatccg cgcccgtacg
     4201 ccgccggatg atcgagccca ttgaagctga cgaactccac gcgctgcgat gcctccgcct
     4261 tcgcggccac gtcgcgcagc ggcgtcacgt cggtcgccga attctcgatg aagacgatgc
     4321 gatgaatcac gtccgccggc agcgcgcagt agaacgaaag cgcctccgcg taatcgcgca
     4381 gccgctcgga aggatcggtc cgccgcaacc ccggcacgcc cggtggcggt gtgatcgtcg
     4441 cggtcagcaa aagcgtgttc atcacaacag ccgcgcagct tagggaccac cgattgggac
     4501 gtaaagagaa acgccgcgat ggcacgagac cggccgcgac gatttcgcct cggctcctat
     4561 cggaattcgc agttcaggtc tttgctgacc caatgaaaaa aggcgcgctt gagcggctat
     4621 caccatcagt gaaggcgccc gcctatcatc cgggtcatga ttcagctctt ttcgccgttg
     4681 aagctccgcg atatcacctt tcgcaatcgg attttcgttt cgccgatgtg ccagtattcc
     4741 agcgatgacg gcatgcccaa cgactggcat ctcgtgcacc tgggcagccg ggccgtcggt
     4801 ggcgcggctt gcgtgatcgt cgaggcgacc ggtgtgtcgc gcgaaggccg catctcgccg
     4861 ttcgacagcg gcatctggtc agacgcgcac gcggcggggt ttcggcggat caccgcattt
     4921 ataaaacagc acggtgcggc gccgggcgtg cagctggcgc acgcgggtcg caaggcgtcg
     4981 accgatgcgc cgtggactgg cggcaagccc gtgccgccca gctctggtgg ttggcagccg
     5041 atcgcgccca gtccgatccc gttcagcgac ggctatccca cgccgcgcca gatgacgcgc
     5101 ggtgacatcg acgccgtgac aaaggaattc gtcgccgcca cccggcgaag ccttgacgcg
     5161 gggttcgagc tggtcgaaat ccacatggcc cacggctacc tgctgcactc gttcctctcg
     5221 ccgctgtcga accatcgccg cgatgacttc ggcggctcgc tggccaaccg tatgcgcttc
     5281 ccgctgcagg tgacgcgcgc gatgcgcaat gtgtggccgg cacatctgcc gctgctggtg
     5341 cgcatcagcg ccagcgattg ggcggacggc gggtgggatt tgccgcagtc gatcgcgttc
     5401 gcccgcgagt tgaagaagct gaacgtcgat ttgatcgact gctccagcgg cggcgcggtg
     5461 ccgtatgcga agatcgaagc cggaccgggt tatcaggtgc cgttcgccag cgccattcgg
     5521 cgtgatgccg gcatcgccac cggcgcggtg ggcatgatca cggatgcgca gcaagccgaa
     5581 tcgatcatcg ccaacggcga ggcggatatc gtgctgctgg cgcgggcgat gttaaaagat
     5641 ccatactggc cgatgcacgc ggcggatcag ctgggaatga agatcgagta ttggccgaag
     5701 cagtatgggc gggcgcggtg atgtcggcgc cgtcccacgg cggagccgcg ggctttggtg
     5761 gtgtcaccgc atttggcgcc tatcatctaa cgatcatgcg caaatcgatt ccatggctcg
     5821 cggtccttct cttttctgga gcaatcatca tggcactgag cactttcttc ggcgcgccca
     5881 aggcgggcgc tgccgccgcc gggccgttgc cggcgccggc tgtggatctg ccggccgaca
     5941 aggatgccaa gcccggcgag cagcacacca ccgtcgtcgc cggtggatgc ttctggtgta
     6001 tcgaaggcgt cttccgccaa ctcaacggcg tgaccaacgc cgtctccggt tacgccggtg
     6061 gatcgaagga gacggcgaat tatgaaaccg tctgcaccgg cgctaccggt cacgccgaag
     6121 cggtgaagat cacctacgac ccgacgaaga tcacctacgg ccaactgctt cgcgcgctgt
     6181 tcaccgtgat cgatccgacg acgaagaacc gccagggtcc cgacagcggc acgcagtatc
     6241 gctcggcgat cttttacgag aatgacgatc aacgccgcgt cgcgcaggcg tacatcgatc
     6301 aactcaacgc ggcgaaggtg tttccgtatc cgatcgtgac gacgctcgag ccgctgaagc
     6361 cggaggcctt ttacccggcc gaggcgtatc accagaacta caccgcgtgt cacttgaaca
     6421 acccgtacat cattcaagag gcgctgccga aggtggagaa gatccgcgcg gcgatgcccg
     6481 acgcggtgaa accggcgacg acgcagccgt gatggaacct tgatgaagaa gcaaagcccg
     6541 cgcatagcta tgcgtgggct ttttgctttg tttatcggac ttctgcggca cccggcgctg
     6601 gacgatccgc gtctgctatg ctaccgatct tcagcgctgc ctatgccccc gccgatgtta
     6661 ccggcgggaa gacgcgttgc gcccgcatga ccaacacccg aacaaagtcc gtccccccgc
     6721 tgcgcgcggc cgccatcggc accggaaaaa tctccgccga gcacctgcgc tacctcagcg
     6781 gcgccggcgc tcgtgccggc gtcagcaagc tggtcggcgt gtgcgatctc tcgccgtcga
     6841 tggcaaactt cgccgccgag gaattcggcg ctgacggcgc gttcacggat tacgcgaaga
     6901 tgctcgccga cgcgaagccg gacgtcgtgc acatcctcac tccgccgcac acgcacgccc
     6961 ggttggtcgc cgactgcctc gaccgcgggg cgcacgtcat tgtcgaaaag cccgtcgcgc
     7021 cgacccacga cgaattcctg tcgctgtggg aacgttcgca acgaacgggt cggcgtttga
     7081 tcgaagatca caactaccgc ttcaacgagc cgatcctcgc gattgaaaag ctattagccg
     7141 acggaaagct cggcgacgtg cgcgaggtgg aagtgcgcat ggccctggcg gtgcgcgaca
     7201 agggcagccg gtacatggac caaagtttgc cgcacccgag ccatcggctg ccgtgcggga
     7261 tcatccacga atttatcacg cacctgtgtt acctggcgct gcggttcatg ccgaaggatg
     7321 cggcattcga ccgcgtcgcc gccgcgtgga gcaagcatgg gtccgatgcg ctcttcaagt
     7381 tcgacgatct ggatgcgacg gtgatcaccg gcgatgtgca tgcgcgattg cggttcacca
     7441 gccacgccgc ccccgattgt ttcgcggtga cggtgcgcgg atccaaagga tacgctgaga
     7501 ccgatctgtt tcagccacac ctgcgcgtcg cgatgc
//
LOCUS       DATIMH010001154         4497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2012733, whole genome shotgun sequence.
ACCESSION   DATIMH010001154 DATIMH010000000
VERSION     DATIMH010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012733"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VLI90_19895"
     CDS             complement(<1..788)
                     /locus_tag="VLI90_19895"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSV16536.1"
                     /translation="MSKAVILQHVPHESPGRIVPVFRDFGIPVETRHLYRGDEVPADL
                     DELRVLVVMGGPMGVADAANASYPFLQKEIDLLKRLVRHDRPVLGICLGAQLLAHAAG
                     AKVYPNVKMAPPAGPGQPPQPAEPLTPLPEIGWAPVTFPFPGGTEPMVMGTHDGAPFF
                     HWHYDTFDLPKLPPPANPAPPPAPPPPTGNALLSSTKLCRNQAFRFKNRLFGFQYHIE
                     STPEGIEAMLANGREDVIKVLGPDGEAKIREDTKKNYAVYERLS"
     gene            complement(951..1547)
                     /locus_tag="VLI90_19900"
     CDS             complement(951..1547)
                     /locus_tag="VLI90_19900"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HSV16537.1"
                     /translation="MTRAIIVVLLAMLLIASGCVPEAKPRDTSLDASAGVNIVPEAIL
                     ASEKITPSTQPSDDSRPDLISVLSRSPEHHEKFLQALRMAGMVPMLREKGPYTLFAPT
                     DEAFAKLPPGTLDRLLQRPDRLRALLQYHILTGRIPFAQILQTNGQVATVAGEKQKLI
                     IRGIGRKVMVNDANVIQSEQSASNGVIHWIDGVLLPPS"
     gene            1743..3227
                     /locus_tag="VLI90_19905"
     CDS             1743..3227
                     /locus_tag="VLI90_19905"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HSV16538.1"
                     /translation="MHPLLQPYIPTENDPFDALKAAHLLNRAGFGGTPSEIEAIMNLG
                     PQAAVDQLLDFPDAGAEEQSQTDVPNLTSIAEYPSTYRELQRSLVGKTDDEKKEIRQK
                     VMNANREALQATVDWWMQRMAFGPHPLQEKLTLFWHGHFVTSAKDERSASLMWKQHEL
                     LRENAAGNFRHFVRAISRDPAMLDYLNNTQNRKAHPNENYGRELMELFTLGIGNYTET
                     DVKEAARAFTGWAHDGDDFIFRKFDHDDGEKTFLGHTGNFDGDDVIEIILSQPVCARF
                     IGAKLFRYFAYEDVDDTLAEGLAQILRGNRWDLRPVVRTILTSKAFYSREAIGTQIKS
                     PIQLVLGTVRLLELQMPPQRALMGALTQMGQLPFAPPNVRGWPGGRMWISTSTMFVRY
                     NTAVFLAGGGSAAFPKLQGKKFGAVGKRNNTVEVAGTNFSPVASTGDGDALVDEWIAR
                     LIQRPVSEEKRKVLTDALAGRPDKPDNVRKMIQLIVSMPEYQLC"
     gene            3348..>4497
                     /locus_tag="VLI90_19910"
     CDS             3348..>4497
                     /locus_tag="VLI90_19910"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HSV16539.1"
                     /translation="MQKIISTRRIFLQRGLTLLAAVPTIPSFLDQTVLALAGPMDTPL
                     TQQPSGKDGKILVVVQLGGGNDGLSTIVPHGDDAYRRARPVIGMDGKTVLKINDYVGM
                     HPNLAPLKAMYDDGHLGIVQGVGYPNPNRSHFRATDIWESGQPDKDIVTSGWVGRYFD
                     NTCPGCDPHVGVSIGQALPLAMQGERIRPLSLERPEAYRYQGRDRAAYEKLNHPATDH
                     ADDLMPNTLAETTKFRKKVEVTSATQLDFLERTAMDAQVSSDDIIRMTQAHRGASGYP
                     GGEFGNAMQTIAAMIAGGLQTRVYYATLTGFDTHANQKPRHDQLMTQLAGGLSAFWKD
                     LKDQGNQDRVLVMTFSEFGRRVTQNASGGTDHGTAAPMFLIGPKITPGL"
BASE COUNT          880 a         1425 c         1415 g          777 t
ORIGIN      
        1 ttgctgagcc gttcgtagac cgcgtaattc ttcttcgtgt cttcgcggat cttcgcttcg
       61 ccgtcggggc cgagcacttt gatcacgtct tcccggccat tggcgagcat cgcttcgatt
      121 ccctccggcg tgctttcgat gtggtattgg aatccgaaca gccggttctt gaaacggaac
      181 gcctggttgc ggcacagctt cgtggaactg agaagggcat tgccggtcgg cggcggcgga
      241 gccggcgggg gcgcggggtt ggccgggggc ggcaacttgg gtagatcgaa cgtgtcgtaa
      301 tgccaatgga agaacggcgc gccatcgtgc gtgcccatga ccatcggctc ggtcccgccg
      361 gggaagggga aggtgaccgg tgcccagccg atctccggca gcggcgtcag cggctccgct
      421 ggttgcggcg gttgaccggg gcccgccggg ggggccatct tcacgttcgg atagaccttc
      481 gcgccggcgg cgtgggcgag caattgcgcg cctaaacaga tccccagcac cgggcggtcg
      541 tgccgcacaa gtcgtttcag cagatcaatt tccttctgca ggaacggata gctggcgttg
      601 gcggcgtcgg ccacgcccat ggggccgccc atgacgacca gcacgcgcag ttcgtccagg
      661 tcggccggca cctcgtcgcc gcggtaaagg tggcgggttt cgaccgggat gccgaagtcg
      721 cggaagacgg ggacgatgcg ccccgggctt tcgtggggga cgtgctggag gatgacggcc
      781 ttactcatgg cggaaaagct aatgcttatc gtgtgaggcg taaagggagg gggtaaccaa
      841 tgtgggtggg gtaattcgca acgcataatt cgtaatgcgt aacgcgaagg ccagatttct
      901 gagatctgtt acgcgttacg atttacggat tgcgcgttac gaattacgca ctacgacggc
      961 ggtagcagca cgccatcgat ccaatgaatc accccgttcg aagcgctctg ctccgactgg
     1021 atcacgttcg cgtcgtttac catcaccttg cggccgatgc cgcggatgat cagcttctgc
     1081 ttttcacccg cgaccgtggc gacctgcccg ttggtttgaa ggatctgcgc gaacgggatt
     1141 cgtccagtta aaatgtgata ttgcagcagc gcccgcaggc gatccggccg ctgcaacagg
     1201 cgatcgagcg tgcccggtgg cagcttggca aacgcttcgt ctgtcggcgc gaacagcgta
     1261 tatggcccct tttcgcgcag catcggcacc atgccggcca tgcgaagcgc ctgcaggaat
     1321 ttctcgtgat gctcgggcga gcgcgaaagg acagagatca agtccggtcg agaatcgtcg
     1381 ctcggctggg tggacggcgt gattttttcc gacgccagga tcgcttcggg cacgatattg
     1441 acgccggcgc tggcgtcgag gctggtgtcg cgcggcttgg cctcgggcac gcagccggac
     1501 gcgatgagga gcatcgccag cagaactacg attattgccc gggtcatcgg cggctcagga
     1561 tacaccaagc cactgggact gaggagcccc gaaccgccgc attcatcgcc gtatcgatgt
     1621 tccggcgttg aaatggcgga agcttgccgc cccaatgcgt ggtacaataa gccgtccgcg
     1681 gccgcgtttg agtctgtggt tagagtgctc tcgaacacgg ttctgtccca agaagaccgc
     1741 ccatgcaccc gttgctccag ccttacattc cgactgagaa cgatcccttc gacgcgctga
     1801 aggcggcgca cctgctgaac cgcgccggct tcggtggcac gccgagcgag atcgaagcga
     1861 tcatgaacct cggcccgcag gcggcggtgg atcagctgct cgacttcccc gacgccgggg
     1921 ccgaagagca gagccagacc gatgtgccga acctgacttc aattgccgaa tatccgtcga
     1981 cgtatcgcga gcttcagcgg tcgctggtcg ggaagaccga cgatgagaaa aaggaaatcc
     2041 gccagaaggt gatgaacgcc aatcgcgagg cgctgcaggc gacggtcgat tggtggatgc
     2101 agcggatggc gttcggcccg cacccgctgc aggaaaagct cacgctgttc tggcacgggc
     2161 acttcgtcac cagcgccaag gacgaacgca gtgcatcgct gatgtggaag cagcacgagc
     2221 tgctccgcga aaacgcggcg gggaatttcc gccacttcgt ccgcgccatc tcgcgcgacc
     2281 cggcgatgct cgattacctc aacaacaccc agaaccgcaa agcgcatccg aacgaaaatt
     2341 atggccgcga gttgatggag ctgttcacgc tcggcatcgg gaactacacc gagaccgacg
     2401 tgaaagaagc cgcccgcgcg ttcactggtt gggcgcacga cggcgatgat ttcatcttcc
     2461 gcaagttcga ccacgacgac ggcgagaaga ccttcctcgg ccacaccggc aactttgacg
     2521 gcgacgacgt catcgagatc atcctcagcc aaccggtttg cgcgcgcttc atcggcgcga
     2581 aactgttccg ctacttcgcg tacgaagacg tcgatgacac gctggccgag gggctggcgc
     2641 agatccttcg cggcaaccgc tgggaccttc gcccggtggt tcgcacgatc ctcaccagca
     2701 aggcgttcta ttcacgcgag gcgatcggca cgcagatcaa gagcccgatc cagctggtgc
     2761 tcggcacggt gcgattgctc gaacttcaaa tgccgccgca gcgcgcgctg atgggcgcgc
     2821 tgacgcaaat gggtcaactg cccttcgcgc cgccgaacgt gcgcggctgg ccgggcggcc
     2881 gcatgtggat cagcaccagc acgatgttcg tgcgctacaa caccgccgtc ttcctcgccg
     2941 gtggcggctc ggcggcgttc ccgaaactgc agggcaaaaa gttcggcgcg gtcggcaagc
     3001 ggaacaacac cgtcgaagtc gccggcacga acttttcccc cgtcgcctcc accggcgatg
     3061 gcgatgcact ggtggacgaa tggatcgcgc gattgattca acggccagtc agcgaagaaa
     3121 aacgcaaggt gctgaccgac gcgttagccg gccggcccga caagccggac aacgtgcgga
     3181 agatgattca gctgatcgtg tcgatgccgg aatatcagct ttgttgaagt cgtgcgcatc
     3241 aagcacggcc cggacgcccg tgtcaccgaa gcgcactaag aaatgttcat cgtgacacgg
     3301 gcctccggcc cgtgcgtgcg gcacgaaagt acgaaggacg accgcccatg cagaaaataa
     3361 tctccacccg ccgcatcttc cttcagcgcg gcctcacgct cctggccgcg gtgccgacga
     3421 tcccgtcgtt cctcgaccag accgtcctcg ccctcgcggg gccgatggac acgccgctca
     3481 cgcagcaacc cagcggcaag gacggcaaga tcctcgtcgt ggtccaactc ggcggcggaa
     3541 acgacggcct cagcacgatc gtgccgcatg gcgacgacgc ctatcgccgc gcccgtcccg
     3601 tcatcggcat ggacggcaag accgtcctga agatcaacga ttacgtcggc atgcacccga
     3661 acctcgcccc gctgaaggcg atgtacgatg acggccacct cggcatcgtg cagggcgtcg
     3721 ggtatcccaa tccgaatcga tcacacttcc gcgccaccga catctgggaa tccggccaac
     3781 ccgacaagga catcgtcacc agcggttggg tcggtcgcta cttcgacaac acctgccccg
     3841 gctgcgatcc gcacgtcggc gtctccatcg gccaggcatt gccgctggcg atgcagggcg
     3901 agcgcattcg cccgctgtcg ctcgaacgtc ccgaagcgta tcgctatcaa ggccgcgatc
     3961 gcgcagctta tgaaaagctg aatcatcccg cgaccgatca cgcggacgat ctgatgccca
     4021 acacgctggc tgagacaacc aagttccgca agaaggtcga ggtcacgtcg gccacgcagc
     4081 tggatttcct cgagcgcacc gcgatggacg cgcaggtcag cagcgacgac atcatccgca
     4141 tgacgcaagc ccaccgtggc gcgagtggtt atcccggcgg ggaattcggc aacgcgatgc
     4201 agacgatcgc cgcgatgatc gccggcggct tgcagacgcg cgtgtactat gccacgctca
     4261 ccggcttcga cacgcacgcc aaccaaaagc cgcggcacga tcagctcatg acgcaattgg
     4321 ccggcggcct cagcgcgttc tggaaagact taaaagacca gggcaatcag gaccgcgtgc
     4381 tggtcatgac gttcagcgaa ttcggccgcc gcgtcaccca aaacgccagc ggcggcaccg
     4441 accacggcac cgcggcgccg atgtttttga ttggtccgaa gatcacgccg ggcctgc
//
LOCUS       DATIMH010001155         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2873443, whole genome shotgun sequence.
ACCESSION   DATIMH010001155 DATIMH010000000
VERSION     DATIMH010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873443"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VLI90_19915"
     CDS             complement(<1..658)
                     /locus_tag="VLI90_19915"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSV16540.1"
                     /translation="MANSPVYEFICGINDVLMEHNQFLVMMKLHQLKPQVVAVSSEPE
                     AVGIARSPRVIAERFVDGLIVETGLPEDLELTVALYGIPTIWLNTDHHHPSDCIYPDE
                     VHAGRLITEHLIGLGQRRIAFLAPSGYRNPLAAFSLVDRQLGYEQAMPAHGLTVRAAQ
                     EESSGSLNMNQTLARLLDPKRTPEPITAIVTYDLSQTLKLRWQVQQTGLSCPRDVSMA
                     A"
     gene            complement(607..1008)
                     /locus_tag="VLI90_19920"
     CDS             complement(607..1008)
                     /locus_tag="VLI90_19920"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV16541.1"
                     /translation="MFRPAVQGASDGREVSLCEIDRDRLWNESRRLGVERTFNELSAV
                     LRSDVDAVALFTQRWVHAPMALEALAAGKHVYSAVPAATTLVELAQLIDAVQRTGLTY
                     MMGEISIYYGSRLFDWPTRPSMNSSAASTMC"
     gene            complement(1324..2700)
                     /locus_tag="VLI90_19925"
     CDS             complement(1324..2700)
                     /locus_tag="VLI90_19925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSV16542.1"
                     /translation="MHSFYRTTRTAFRALRRNVMRSALTCLGIIIGIAAVIAMVEIGH
                     GSSHAMEQTIEGMGANVVQVDPSDVIKAGVSSGSGGRPTLTPTDAEAIARECSAARWV
                     APSVDCRAQMIYGSRNWAPHRILGTTPQYLPIRKWDDLAEGAPFTDEDVRRAAPVCIL
                     GQTPARELFQGKSPIGKEVRVKNVRMRIVGVLTAKGANMIGYDQDDFVIAPWTTVKYR
                     LSNSKFAFSSAGGSSSSSSSAMNTLNQIYPSQSVQLYPPTSTVQATDTPMPIRFNDLD
                     DIYVAANSADDIPLAIHQITEVLRDRHRLKDGQMDDFRVRDWAEMSKALASSTTVMTN
                     LLLCVALISLVVGGVGIMNIMLVSVTERTREIGLRMAVGARAKDILRQFLLEAILLCL
                     AGGIAGIALGRGVSIAVKTFLNWPTLLSMPAIIAAVAVSATVGIVFGYYPAWKASSLD
                     PIEALRYE"
BASE COUNT          515 a          933 c          887 g          515 t
ORIGIN      
        1 cggccgccat ggaaacgtcg cgcgggcagc tcaatccggt ctgctgcacc tgccagcgca
       61 gcttcagcgt ctgactcaga tcgtacgtga cgatcgccgt gatcggttcc ggcgtgcgct
      121 tcggatccag caggcgcgcc agcgtctggt tcatgttcag cgaaccggaa ctttcctctt
      181 gggccgcgcg cacggtcagc ccatgcgcag gcatcgcctg ctcgtaaccc agctgccgat
      241 cgaccagcga aaacgccgcc agcggattgc ggtatccgct cggcgccaga aatgcgatcc
      301 gacgctgacc cagcccgatc agatgctcgg tgattaatcg ccccgcgtgt acttcgtctg
      361 gataaatgca gtcgctgggg tggtgatggt cggtgttgag ccagatcgtc gggatgccgt
      421 acagcgcgac ggtcaactcc aaatcctccg gcaagcccgt ctccacgatc agcccatcca
      481 cgaagcgctc agcgatcacg cgcggcgatc gtgcgatccc caccgcctcc ggttccgatg
      541 acaccgccac cacctgcggt ttcagctggt gcagcttcat catcaccaga aactggttgt
      601 gctccatcag cacatcgttg atgccgcaga tgaattcata gacgggcgag ttggccaatc
      661 gaacagccgg ctgccatagt agatgctgat ctcgcccatc atgtacgtga ggcccgtgcg
      721 ctgcaccgcg tcaatcagct gcgccaactc caccagcgtc gtcgccgccg gcaccgccga
      781 atagacatgc ttccccgcgg ccagcgcttc cagcgccatc ggcgcatgca cccatcgctg
      841 cgtgaacagc gcgaccgcat ccacgtcgct gcgcagcacc gccgacaact cgttaaacgt
      901 ccgttccacg cctagccggc gagattcgtt ccacaaccga tcgcgatcga tctcgcaaag
      961 cgaaacctct cgaccatcgg atgccccttg tacagcggga cgaaacactg cgcgaactgc
     1021 ccgcgccgac gatgcatact ttgatccgct tcaagactca tctcctccgt cacggccgtc
     1081 gtcaccaatt cgcacgaatt caattgtggc tgccgatgtc gatgatgctc ggtcgatcgc
     1141 gaacagacgg ggttgccggt gccttccgcc tgctgatcac gtgcagttta ccaagtcgat
     1201 tcgaagtagg cagatggacg tcaatgctca tgcctggcgg cgccgccggg atcgcgatcc
     1261 gcgatggagc ggtcgtgaga ttggcgcagg atcgtgaatg aaattctcgc gccaggcccg
     1321 caatcactcg taccgcagcg cttcgatcgg gtcgaggctc gacgccttcc acgcagggta
     1381 gtagccgaag acgatgccga cggtggctga caccgcgacg gcggcgatga tggcgggcat
     1441 ggacaggagc gtgggccagt tcagaaatgt cttgaccgcg atggagacgc cgcggcccag
     1501 ggcgatgccg gcgatgccgc cggcgagaca gagaaggatc gcttcgagca ggaactggcg
     1561 gaggatgtct ttggcgcggg cgccgacggc catgcgcagg ccgatctcgc gcgtgcgctc
     1621 ggtcaccgac accagcatga tgttcatgat ccctaccccg ccgacgacca gcgagatcag
     1681 cgcgacgcac agcagcaggt tggtcatgac ggtggtgctg gacgccagcg ccttggacat
     1741 ttccgcccag tcgcgcacgc ggaagtcgtc catttgcccg tccttcaggc gatggcggtc
     1801 gcgcagcact tcggtgatct ggtgaatcgc cagcggaata tcgtcggccg aattggcggc
     1861 gacgtagatg tcgtcgaggt cgttgaagcg gatcggcatc ggcgtgtcgg tcgcctgcac
     1921 agtggacgtg ggcggataca gctgcacgct ctggctcggg taaatctggt tgagcgtgtt
     1981 catcgccgac gatgacgatg atgaagaacc accggcggag gagaaggcga acttcgaatt
     2041 cgacaggcga tacttcaccg tcgtccacgg cgcgatgaca aagtcgtcct ggtcgtagcc
     2101 gatcatgttc gcgcccttgg cggtgaggac gccgacaatc cgcatgcgca cgttcttgac
     2161 ccgcacttcc ttgccgatcg gcgacttgcc ctggaacagc tcgcgggcag gcgtctggcc
     2221 caggatgcag accggggcgg cgcggcggac gtcctcgtcg gtgaaggggg cgccctccgc
     2281 caggtcgtcc cacttgcgga tcggcaggta ctgcggcgtc gtcccaagga tccggtgcgg
     2341 ggcccagttg cggctgccgt agatcatctg cgcacggcag tccacgctgg gcgccaccca
     2401 gcgggccgcg ctgcactcgc gcgcgatcgc ctcggcatcg gtgggcgtca gcgtcggtcg
     2461 cccgcccgac ccgctgctca cccccgcctt gatcacgtcc gaagggtcca cttgcacgac
     2521 gttcgccccc atcccctcaa tcgtctgctc catcgcgtga gatgacccat gcccgatctc
     2581 caccatcgcg atcaccgccg cgattccgat gatgatcccc aggcacgtca gcgccgagcg
     2641 catcacgttc cgccgcaggg cgcggaacgc ggttcgggtt gtccggtaaa acgagtgcat
     2701 cttcgggttg cttcgggcat gtgcccgagt tcggctgcgc caggccacgc gcccggaaac
     2761 gcatggcgga tcttatcatt tttcgacgat catgggggaa aaagcgggag cgttggtgtg
     2821 atattccagg gcggaatcat tcgggagcgc
//
LOCUS       DATIMH010001156         1685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2012863, whole genome shotgun sequence.
ACCESSION   DATIMH010001156 DATIMH010000000
VERSION     DATIMH010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012863"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..>1685
                     /locus_tag="VLI90_19930"
     CDS             174..>1685
                     /locus_tag="VLI90_19930"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HSV16543.1"
                     /translation="MALKKAPAKRAGKSKVAKAKGSSGANGVNGKLTKKRVYFFGNGK
                     AEGGAHLKDLLGGKGANLADMTLVPLPVPPGFTITTETCAEYNNGGQKLPRGLMDDVR
                     TNITKVEKATGKKFGDVNNPLLVSARSGAKMSMPGMMDTVLNIGLNDRVVEGLARLSG
                     NERFAYDSYRRLINMFGDTVMGVDHHHFEHELTAVKNNRGAKVDTDLDVDALKEVVQR
                     YKQVYKQHVGSDFPQDPFTQLEHAIEAVFKSWMGDRAIRYRQLNDIRGLAGTAVNVQA
                     MVYGNMGDDSATGVAFTRNPSTGGNKFYGEFLINAQGEDVVAGIRTPIPCEQMGEWSQ
                     KSWKELLKIKDVLEKRYKDVQDFEFTIEKGKLYMLQTRNGKRTAPAAVRIAVEMIKEK
                     LIDEKTGILRVDPASLDQLLHPTFDPKAARNVLARGLPASPGAAVGKIAFTAEEAEKR
                     VAGGEKIILVRRETEPADIGGMHVSEGILTSTGGMTSHAAVVARGMGTPCVAGA"
BASE COUNT          381 a          490 c          544 g          270 t
ORIGIN      
        1 caccaaagcc cgcgaaagcc cacggttatg ccgtgggacg gattttcaaa aacgccggtc
       61 ccacggcaga gccgcgggct ttgacatgaa aatgcgccgt tcctatactg cgcccttttc
      121 cactttttcc aaggttttaa cgggatttcc cgcaggagaa cgagatcatg gcgatggcgt
      181 tgaagaaggc cccggccaag cgtgccggca agagcaaggt ggctaaggcc aagggttcca
      241 gtggtgcgaa cggagtgaac ggcaagctga ccaaaaagcg agtctacttc ttcggcaacg
      301 gcaaggccga aggcggcgcc catctgaagg acctgctggg cggcaagggc gcgaacttgg
      361 ccgacatgac gctcgttccc ctgcccgtgc cgccgggctt caccatcacc accgaaacct
      421 gcgcggagta caacaacggc gggcagaagc tgccgcgcgg gctgatggac gacgtccgca
      481 cgaatattac gaaagtcgaa aaagcaactg gtaagaagtt cggcgacgtc aacaacccgc
      541 tgctggtctc cgctcgctcg ggcgcgaaga tgtcaatgcc gggcatgatg gacacggtgt
      601 tgaacatcgg gctgaacgac cgcgtcgtcg aaggcttagc gcgattgagc ggcaacgagc
      661 gcttcgccta cgacagctat cgccggttga tcaacatgtt cggcgacacc gtcatgggcg
      721 tcgatcacca tcacttcgag cacgagctga ccgccgtcaa gaacaaccgc ggggcgaagg
      781 ttgataccga tctcgacgtg gatgcgctga aggaagtcgt gcagcgctac aagcaggttt
      841 acaagcagca cgtcggcagc gattttccgc aggacccgtt cacgcagctg gagcacgcga
      901 tcgaagcggt gttcaagagc tggatgggcg atcgcgccat ccgctatcgc cagctgaacg
      961 acatccgcgg gctggccggc acagccgtca acgtgcaggc gatggtttac gggaatatgg
     1021 gcgacgacag cgcgaccggc gtcgcgttta cgcgcaatcc gtccaccggc gggaacaagt
     1081 tttacggcga gttcctgatc aacgcacaag gcgaagacgt cgtcgccggc attcgcacgc
     1141 cgattccgtg tgagcagatg ggcgagtgga gccagaagag ctggaaggaa ctgctgaaga
     1201 tcaaagacgt attggagaag cgatacaagg acgtgcagga tttcgaattc acgattgaaa
     1261 agggcaagct gtatatgctg cagacccgca acggcaagcg gaccgcgccc gcggccgtgc
     1321 gcattgcggt agagatgatc aaggaaaagc tgatcgatga aaagaccggc atccttcgcg
     1381 tcgatccggc ttcattggat cagctgctgc acccgacgtt cgatccgaag gcggctcgca
     1441 acgtgctggc acgcggcctg cccgcctcgc ctggcgcggc ggtgggcaag atcgccttca
     1501 ccgcggaaga agcagaaaaa cgcgtggccg ggggtgagaa gatcatcctg gtccgtcgcg
     1561 agaccgaacc ggccgacatt ggcggcatgc acgtctcgga aggcatcctg accagcaccg
     1621 gcggcatgac atcgcacgcg gcggtcgtcg cgcgcggcat gggaacgccg tgcgtcgccg
     1681 gcgcg
//
LOCUS       DATIMH010001157         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5478185, whole genome shotgun sequence.
ACCESSION   DATIMH010001157 DATIMH010000000
VERSION     DATIMH010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5478185"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..>2161
                     /locus_tag="VLI90_19935"
     CDS             255..>2161
                     /locus_tag="VLI90_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16544.1"
                     /translation="MRRPLRAILITTLLAALAAAAPPASQPLTGDAVLQHLDSTIDWY
                     RRVTAFGQSPVNSQELLLRESARQNARQAVRLALDFGRAEASLLESSQASTTPSSKSG
                     SASRIEQATTRAAGRVTELQDQLAQLDQQIKSAAPDAVEGLKSRRDHLNAQLNLVSAQ
                     RDTLNEYSRFVTNASSNGGGDTLLKRIDDLAQSVPEVQEDTADQKAQNTTSSASTASA
                     TAAATAAAAAAQPSVHPESSGVFTLISHMFTLSSRMHELKGFTEEAGKLRDHADAFRG
                     PIRNDLVNAVHRGDAIAASTQPVDDPQQLDAEREELNSLTARFKALAAAAVPLGEQSL
                     MFDAARQNLINWRDALEREYARALRTLLIRASALIITILVLIGISELWRRATFRYIED
                     VRRRRQLMTVRRIVIAVVVFIVIIANVVSEFGSLATFAGLITAGIAVALQTVILSGVA
                     YFFFIGRFGVRVGDRVTINNITGDVVEIGLFRIYLMELSGPKTDPHATGRIVVFSNSV
                     LFQSQAFYKQLPGADYVWHEITFTLAPDTDYKLAEDRLLGAVEAVFSEYRDDIQRQYE
                     SVATSLQIQMPPPRPQSRLRFVDAGLEFAVRYPVEMRRASEVDEKITHKLLATIEQEP
                     KLKLVASTTPK"
BASE COUNT          394 a          750 c          670 g          347 t
ORIGIN      
        1 gatctggccg agaagtaagt gaaccgccgg cggcaggaac gctatggcag tcgcttgcgg
       61 tttcgcagac caatgaccac gggttgtccg tctgcgaagc cgcaagcgac tgcggcgccg
      121 ttcgcgcgga tggcgcttca cctacgtctc ggttagatcc ttcggctccg gcggaggacc
      181 gcctgcgctc aggatggcgc gagagatttt ccccgctgtc agccgacgcg cgggcgcgta
      241 ggttgacgcg ggtcatgaga cgcccgcttc gcgcgatttt gataacgacg ctgctcgccg
      301 ctctcgcggc ggcggcgccg ccggcgtcgc agccgctgac cggcgacgcg gtgctgcagc
      361 atctggattc cacgatcgac tggtaccgcc gcgtcaccgc gttcgggcag tcgccggtca
      421 actcacaaga attgctgctg cgcgaaagcg ctcgccagaa cgctaggcaa gcggttcggc
      481 ttgcgctgga tttcgggcgg gccgaagcat cgctgctcga aagcagccag gcctcgacga
      541 caccgtcgtc caagtccggc agcgcctcgc gcatcgagca agccaccacg cgcgccgccg
      601 gccgggtgac cgagctgcag gatcagctcg cgcagctgga ccagcagatc aagtccgccg
      661 cgccggatgc ggtggaggga ctcaagtccc gccgcgacca tctcaacgcc cagctgaacc
      721 tcgtcagcgc gcagcgcgac acgctgaacg aatactccag gttcgtcacc aacgcatcga
      781 gcaacggcgg cggtgatacg ctgttgaagc gcatcgatga cctcgcacaa tccgtgcccg
      841 aggtgcagga agacaccgcc gaccaaaaag cccagaacac gacatcgagc gcctcgaccg
      901 catcggcaac ggccgccgcc accgcggcgg cggcggcggc gcagccgagc gttcacccgg
      961 agtcctccgg cgtcttcacg ctcatctcgc acatgttcac gctcagctcg cgcatgcacg
     1021 agctcaaagg tttcaccgaa gaagccggca agctgcgcga ccacgccgac gcctttcgcg
     1081 ggcccattcg caatgacctg gtcaatgccg tgcatcgcgg cgacgcgatc gccgcgtcca
     1141 cccagccggt ggatgacccg cagcaactcg acgctgaacg ggaagagctc aactcattaa
     1201 ccgcgcgctt caaagccttg gccgccgccg cggtgccgct gggcgagcag agcctgatgt
     1261 tcgacgccgc ccggcagaac ctgatcaact ggcgcgatgc attggagcgc gaatatgccc
     1321 gcgccctgcg cacgctgctg atccgcgcca gcgcgctgat catcaccatc ctggtgctga
     1381 tcggcatctc cgagctgtgg cgccgcgcga cgttccgcta catcgaagac gtccggcggc
     1441 gccggcaatt gatgaccgtc cgccggatcg tcatcgccgt cgtcgtcttc atcgtcatca
     1501 tcgccaacgt cgtcagcgaa ttcggctccc tcgccacctt cgcggggctc atcaccgccg
     1561 gcatcgccgt ggcgctgcag accgtcatcc tctcgggcgt cgcgtacttc ttcttcatcg
     1621 gccggttcgg cgtgcgcgtg ggcgaccgcg tcaccatcaa caacatcacc ggcgacgtcg
     1681 tcgagatcgg cctgttccgc atctacctga tggagctgag cggccccaag accgacccgc
     1741 acgcgacggg ccgcatcgtc gtcttttcga attcggtgct gttccagtcg caggcgtttt
     1801 acaagcagct gcccggcgcg gattacgtct ggcacgagat caccttcacg ttggcgccgg
     1861 acacggatta caagctggcc gaggatcgcc tgctgggcgc ggtggaagcc gtcttctccg
     1921 aataccgaga cgacatccag cggcaatacg aatcggtcgc gaccagcctg cagatccaga
     1981 tgcccccgcc gcgcccgcaa agccggctgc gcttcgtcga cgccggcctg gaattcgccg
     2041 tgcgctatcc agtcgaaatg cggcgggcat cggaggtgga cgagaagatt acgcacaagc
     2101 tgctggcaac gatcgagcag gaaccaaagc tgaagctggt ggcgtcgaca acaccaaaga
     2161 t
//
LOCUS       DATIMH010001158         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5478572, whole genome shotgun sequence.
ACCESSION   DATIMH010001158 DATIMH010000000
VERSION     DATIMH010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5478572"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="VLI90_19940"
     CDS             complement(<1..1511)
                     /locus_tag="VLI90_19940"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSV16545.1"
                     /translation="MSTTTVPNAQTTGEVKDVQMLRAGKWETSAAKRSGNVFNPSTGK
                     VIARVPFATADETNAAVEAAAAALPAWSQTPAVERARVMFKYRELLLQNFERLASMVT
                     REHGKTMVEARASVQRGIEVVEFACSVPNMLMGEILPNLARNVDAETVRHPVGVCAGI
                     TPFNFPAMVPMWMFPVALVCGNTFVLKPSEKVPLSSNILGELLTEAGCPAGVFNIIHG
                     DKEAVDALLTHPKVAAISFVGSTTIAKYIYEVGSKHGKRIQSAGGAKNHLIIMPDADL
                     DQAVAALQASAFGCAGERCMAGSVAIGVGKIGDELVSRLVDKASKMKVGPTDAGVDVD
                     MGPLISREHRDRVASYLDIGKNEGAAVALDGRQGAPTEGFFLKPSIVDRVKPEMRVAT
                     DEIFGPVLSVIRTGSLEEALEIGRRSPYGNGASIFTQSGYAARQFKQHFNAGMIGINI
                     GVPAPMAWFPFTGWNNSFFGDLHIQGRESVMFYTRGKTIMTRWFEHASEAAHDP"
     gene            1772..>2688
                     /locus_tag="VLI90_19945"
     CDS             1772..>2688
                     /locus_tag="VLI90_19945"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSV16546.1"
                     /translation="MSSPTPSQAVAKHKQYLFPAVAMYYQEPIALVKGEGNYVWDDQG
                     RKYLDAFGGVLTVSVGHANPKVVNAIVEQVKKINHTSTLYANEQQSNLAEKLASISPG
                     NLKKSFFTSSGTEANETAMNAARHATGRLELITLRHSYSGRSAQTLTAAGQSAWKKLP
                     PLVPGIHHAHAPYCYRCPFNLEYPKCGLKCAEDLEELILTETSGEIAAFMAETIIGSG
                     GFIVPPPGYFEKAVAIARKYGGLFICDEVQAAWGRTGEHWWGIQHWDVNPDIMVSAKG
                     MGNGAPVAMTIATPEVADKFPGITFATFGG"
BASE COUNT          488 a          838 c          857 g          505 t
ORIGIN      
        1 ggatcatgcg ccgcttcgct ggcgtgctcg aaccacctcg tcatgatcgt ctttcccctc
       61 gtgtagaaca tcaccgactc tcggccctgg atgtgcaggt cgccgaagaa ggagttgttc
      121 cagccggtga aggggaacca cgccatcggg gcggggacgc cgatgttgat gccgatcatg
      181 ccggcgttga agtgctgctt gaactgcctc gccgcgtatc cactttgcgt gaagatgctg
      241 gcgccgttgc cgtaggggct gcggcggccg atctccaggg cttcttcgag gctgcccgtg
      301 cggatgaccg acaggacggg gccgaagatc tcgtccgtgg cgacgcgcat ctccggcttc
      361 acgcgatcga cgatgctggg cttcaggaaa aagccctcgg tgggcgcgcc ttggcggcca
      421 tcgagcgcga cggcggcgcc ttcgttcttg ccgatgtcga gatagctggc gacgcgatcg
      481 cgatgctcgc gcgagatcag cgggcccatg tccacatcca ccccggcgtc ggtcgggcca
      541 accttcatct tcgaagcctt atcgacgagc cggctgacga gttcatcgcc gatcttgccg
      601 acaccgatgg cgaccgaacc ggccatgcag cgctcgccgg cgcagccgaa ggctgacgct
      661 tgaagcgcgg ccaccgcctg gtccagatcg gcatcgggca tgataatgag gtggttcttg
      721 gccccgccgg cggattgaat gcgtttgccg tgcttgctgc cgacttcgta aatgtatttt
      781 gcgattgtcg tagaacccac gaaggagatc gccgccacct tgggatgcgt cagcagcgca
      841 tcgaccgctt ctttatcacc gtggatgatg ttgaacacac ccgcgggaca accggcttca
      901 gtcagcagtt cgccaaggat gttgctcgac agcggcacct tttcgctggg cttgagcacg
      961 aacgtgttcc cgcaaaccag cgccaccggg aacatccaca tcggcaccat cgcggggaaa
     1021 ttgaacggcg tgatgccggc gcacaccccg acgggatgcc tcaccgtctc cgcgtccaca
     1081 ttgcgggcga gatttggaag gatttcgccc atcagcatgt tcggcacgct gcaggcgaat
     1141 tcgacgacct cgatcccgcg ttgcacgctg gcgcgcgctt cgaccatcgt cttgccgtgc
     1201 tcgcgcgtca ccatgctggc gagccgttcg aaattctgca gcagcagctc gcggtacttg
     1261 aacatgaccc gcgcgcgctc gacggcgggc gtctgcgacc acgccggcaa cgccgccgcc
     1321 gccgcttcaa ccgcggcgtt ggtttcatcc gccgtcgcga acggcacgcg cgcgatgacc
     1381 ttcccggtcg aggggttgaa gacgttcccg ctgcgcttgg cggcgctggt ctcccacttg
     1441 ccggcgcgca gcatctgaac gtctttcact tcacctgtcg tctgcgcgtt gggcacggtc
     1501 gttgtactca tggtgtgact cctattgtcg cggtggcgca agcgctccag tcccctcccc
     1561 cggtactgct gatcgcagat cgccgggagg gggggcaaga cgagaggcgc agcgcgcaaa
     1621 gcgcgcgcat gccgatgata tcgcgccacg tcagaattcc ctagactttg gctaaccaat
     1681 tgttaatgtg gcggggttgt aatcgcgccg tgacacgcat gtttggtgaa cgtcggttaa
     1741 ccctcggaca atcgtcaggc aaccaaatca aatgagttct cccacgcctt cgcaagccgt
     1801 cgctaagcac aagcaatatc tctttccagc ggtcgccatg tattaccagg agccgatcgc
     1861 gctggtgaaa ggcgaaggta attacgtctg ggacgaccag ggccgcaaat atctcgatgc
     1921 gtttggtggc gtgctgaccg tctcggtcgg tcacgccaac ccgaaggtcg tcaacgcgat
     1981 cgtcgagcag gtgaagaaga tcaaccacac ctcgacgctg tacgccaacg agcagcagtc
     2041 gaacctggcg gagaagctgg cgtcgatctc gccggggaat ctgaagaagt ccttctttac
     2101 cagcagcggc accgaggcga acgagacggc gatgaacgcc gcccgccacg cgacgggccg
     2161 gctggagttg atcacgctgc ggcacagtta ttcgggtcga tcggcgcaga cgttgaccgc
     2221 cgcgggacag agtgcgtgga agaagctccc gccactggtg ccgggcattc atcacgcgca
     2281 cgccccctac tgctatcggt gtccgttcaa tctggaatat cccaagtgcg ggctgaagtg
     2341 cgcggaggat ttggaagagc tgatcctgac ggagaccagt ggcgagatcg ccgcgttcat
     2401 ggccgagacg atcatcggca gcggcgggtt catcgtcccg ccgccgggat atttcgagaa
     2461 ggccgtggcc atcgcccgca agtatggcgg gctgttcatc tgcgacgaag tgcaagccgc
     2521 ctggggccgc acgggcgagc actggtgggg cattcagcat tgggatgtga acccggacat
     2581 catggtgtcg gccaagggga tgggcaacgg cgcgccggtg gcgatgacga tcgccacgcc
     2641 ggaggtggcc gataaattcc ccggcatcac cttcgcgacg ttcggcgg
//
LOCUS       DATIMH010001159         5596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_272591, whole genome shotgun sequence.
ACCESSION   DATIMH010001159 DATIMH010000000
VERSION     DATIMH010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272591"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VLI90_19950"
     CDS             <1..1305
                     /locus_tag="VLI90_19950"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HSV16547.1"
                     /translation="RDLNWQITSLKAPMPELHDAPIIYLAGNQALSFAPEEEAKLKQF
                     CEEGGIIFGNADCGNADFATSFRKLGAKLFPGYEFRELPASHPIYTNEQYLRTNWKSP
                     PSPLALSNGVREMMVVQPTADAARYWQADDPSGREEAFQFADNLFLYAVDKQNLLEKG
                     KTYLVYDDERIKPQQRIKMGRLEYDGNWDPEPAGWRRMSALLHNMGDKLELFPIKLGS
                     GKLGNGRGVGVRVAHLTGTTRFKLNLEQRKELKEFAEGGGTLIIDAAGGSGEFADSAE
                     SELAAIFGGDVPNQLKEPLPPADHVYDLPGNKIEDFGYRPFTRKIVGTLRGPQVKAIK
                     VNDRPAVYYSREDLSAGLVGQPVDGIIGYTPTTATAIMRNLILQGGGVGDKPASTQPA
                     PEAAATQPGAPKPPTPPKPKSPSRKPKSTTKPTTKPATKPKT"
     gene            1341..1661
                     /locus_tag="VLI90_19955"
     CDS             1341..1661
                     /locus_tag="VLI90_19955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16548.1"
                     /translation="MTAALIAGGCASTSPGTLVTRQDAHTPPTMTKAQQDGAYGLFIA
                     GESDPVFAVQLRQGDEIGFEQANRATVGSLQIIYLYAVYGGNRYPVNAAKTYEWRWLY
                     AARK"
     gene            2198..3847
                     /gene="cimA"
                     /locus_tag="VLI90_19960"
     CDS             2198..3847
                     /gene="cimA"
                     /locus_tag="VLI90_19960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HSV16549.1"
                     /translation="MDSGRRIEIYDTTLRDGTQGEGFNLSLQDKLLIAKKLDEFGVDY
                     IEGGFPGSNEKDRAFFKEIKPLRLRHAKVSAFGMTRRRGMKAEDDPNMKSLLNAETPV
                     VTLVGKTSDYQARAVMNVTPEENLEMIADSVRLMTSAGRKVVYDAEHFFDTFGSNRDY
                     AVRTLQAAEKAGATVLCLCDTNGGSMPEFVAEAVEFVRTQTSATIGIHTHNDAALAVA
                     NALAAVRAGADHAQGTVNGVGERCGNMDLVPLVANLRLKYNLDCLQSDSLSYLTDLSR
                     YVYETANLNLFSGQPYVGPSAFAHKGGMHVAAVQKDVSTYEHVDPASVGNTRRILVSE
                     LSGISNIATKAGVKFNIENDKAALKNILERVKTLENEGYHFEAAEASFELLLRKEIGR
                     HRSFFNLDHYRTVVLKSGEGVEAISEATVKMRVGDVVEHRVAEGDGPVNALDLALRRA
                     LKPHYPQIDKVHLMDYKVRVINSNDETAAKVRVIIESKREHADGSKEIFGTIGVSGNV
                     IDASWQALVDAYEYHLIHVMEETGAKAPATSAPISEPVGQT"
     gene            3953..5458
                     /locus_tag="VLI90_19965"
     CDS             3953..5458
                     /locus_tag="VLI90_19965"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HSV16550.1"
                     /translation="MDAYDALITELRELALLGSISSALGWDEQTHLPPKGADHRANQI
                     SYLAAMQHRRFTSPRIGELLQAVESSDLVKHSDSDIAVNVRETRRNYDKARKLPESLV
                     EEMSRHSVLSQQAWTEARKRADFPAFVPYLERTLQLKKQEAACIGYTTQPYDALLDAY
                     EPGDTSADIARVFEQLRGPLVDLIRRVGESRRPAPPVEIMQRTFPAAAQEAFAKEAAA
                     RIGFDFDAGRLDVSVHPFCSELGPHDVRITTRYDEHQFNDAFFGVLHETGHGLYDMGL
                     PAEHYGTPLGSFVSMGIHESQSRMWENLVGRSRAFWSFFLPKARETFSEALGGVSDDD
                     WYRAINDIRPSLIRVEADEATYNLHILLRFEMEQAMMSGNLGVGDLPAAWNERMKSYL
                     GLTPPDAATGVLQDIHWAFGGIGYFPTYTLGNLYAAQFFEQARKDLGDLDAQFARGEF
                     RPLLDWLRQNIHRHGQRYKARELVRRVTGKDLTAQPLLDYLNGKASEVYDV"
BASE COUNT         1140 a         1659 c         1856 g          941 t
ORIGIN      
        1 cgcgatttga attggcagat caccagcctc aaggcgccga tgccggagct gcacgatgcg
       61 ccgatcatct atttggcggg caaccaggcg ctgtcattcg ctccggagga agaggcgaag
      121 ctgaagcaat tctgcgagga aggcgggatc atcttcggca acgccgactg cgggaacgcc
      181 gacttcgcaa cctcgtttcg caaattgggg gcgaagttgt ttccaggcta cgaattccgg
      241 gagctgccgg ccagtcaccc gatctatacc aacgagcagt acctgcggac caactggaaa
      301 agcccaccga gcccgctggc gctgagcaac ggcgtgcgcg agatgatggt ggtgcaaccg
      361 accgcagatg cggcgcgata ttggcaggcg gatgatccgt cgggccgcga ggaggcgttt
      421 cagttcgccg acaatttgtt cctgtacgcg gtggacaaac agaacctgct ggagaagggc
      481 aagacgtatc tcgtttacga cgacgaacga atcaagccgc agcagcggat caagatgggc
      541 cggctggagt acgacgggaa ctgggacccg gagccggcgg gctggcggcg gatgtcggcg
      601 ctgctgcaca acatgggcga caagctcgag ttgttcccga tcaagctggg cagcgggaaa
      661 ctcggcaacg gccgcggggt cggcgtgcgg gtggcgcatc tgaccgggac gacgcgcttc
      721 aagttgaacc tcgaacagcg caaggaactg aaggaatttg ccgaaggcgg cgggacgctg
      781 atcatcgacg ccgcgggtgg atcgggcgaa tttgccgact cggccgagag cgagctggcc
      841 gcgatcttcg gcggcgacgt gccgaaccaa ttgaaggaac cgttgccgcc ggcggaccac
      901 gtttatgatt tgccggggaa caagatcgaa gacttcggct atcggccgtt cacgcggaag
      961 atcgttggca cgctgcgcgg gccacaggtg aaggcgatca aggtgaacga tcgcccggcg
     1021 gtgtattaca gccgcgagga tttgagcgcg gggctggtcg gtcaaccggt ggatggcatc
     1081 atcggctaca cgccgacgac ggcgacggcg atcatgcgca acctgatcct gcaaggcggc
     1141 ggggtgggcg ataaacccgc gtcaacgcag cccgcgcccg aggcggcggc gacgcagccg
     1201 ggcgcaccca agcccccaac accgccgaaa ccaaagtcgc cgtcgaggaa gccgaagtcc
     1261 acgacgaagc cgacgacgaa accggcgacg aaacccaaga cgtgatgtcc accaaacgcc
     1321 aatcgaagtt gagtgtgata atgaccgcgg cgctgatagc cggcgggtgc gcttcaacgt
     1381 cacccggcac gctggtcacc cggcaggacg cccacacgcc gccgacgatg accaaagccc
     1441 agcaagacgg cgcgtacggc ctgttcatcg ccggcgagtc ggaccccgtc ttcgcggtgc
     1501 aactgcggca gggggatgag atcggcttcg aacaagcgaa tcgggcgacc gtgggttcgc
     1561 tgcagatcat ctatctctac gcggtgtacg gcgggaatcg atatccggtg aacgccgcca
     1621 agacctacga gtggcggtgg ttgtacgcgg cacgaaaatg agtccgcgca ccatcaggcc
     1681 gtctgaacgt gtgccgcgaa gggtgtagcg tacgattctt ggtatgcgtc tattagcgcc
     1741 aagaggccaa gacgccaggg aagagaaaga cagaatgtgt gaagcgctgg gcgcgcgctc
     1801 gggcgttttt cgccgcgtgc cgaggtgcct tgcaccgggc gagagtgatt ttgaatttcg
     1861 atagccgctg gcactcgaaa tcaccctcac cctaaccctc tcccggcgac ctgcggtcag
     1921 cagtaccggg agaggggacc agagcgcgcg gttgctatat gcgattgccc tgcgctgcac
     1981 ccaccgcgga gtgccggcgc gtttgactct cgccccgatt tggttatcat cccgcgatca
     2041 tgttctcccg cattgcgacc agccggatta tcattacccc acgacaacgc gggtagggct
     2101 gatcgtttgc cgtttgcttc tgagaagtgc tgagcagccc tgccccacgc ggcagggcgt
     2161 tttcgtttgt ggatgttcta acctggcagg tgcgacgatg gattcaggac gccgaatcga
     2221 gatctacgac acgacgcttc gcgatggcac gcagggcgaa ggctttaacc tgtcgctgca
     2281 ggacaagctg ctgatcgcga agaaactcga cgagttcggc gtcgattaca tcgaaggcgg
     2341 gttccccggc tccaacgaaa aggaccgcgc gttcttcaag gagatcaagc cgctgcgcct
     2401 gcggcacgcg aaggtgtcgg cgttcggcat gacccgccgg cgcggcatga aggccgaaga
     2461 tgatccgaac atgaagtcgc tgctgaacgc ggagacgccg gtggtcacgc tggtgggaaa
     2521 gacgagcgat taccaggccc gcgcggtgat gaacgtcacg ccggaagaga atctggagat
     2581 gatcgccgac tcggttcggc tcatgacctc cgccggtcgc aaggtcgtct acgacgccga
     2641 gcatttcttc gacaccttcg gcagcaatcg cgattacgcc gtgcgcacct tgcaggcggc
     2701 ggaaaaagcc ggggcgacgg tgctgtgcct gtgcgacacc aacggcggat cgatgccgga
     2761 gttcgtcgcc gaagccgtcg agttcgtgcg gacgcaaacg tcggcgacga tcggcattca
     2821 cacacacaac gacgcggcac tggcggtggc caacgcgctg gccgcggttc gcgccggcgc
     2881 ggatcacgcg caagggacgg taaacggcgt cggcgagcgc tgcggcaaca tggacctggt
     2941 gccgctcgtc gccaatcttc gcctcaaata caacctcgat tgtcttcaat ccgattcgct
     3001 ctcctacctc acggacctca gccgctacgt ttacgagacg gcgaatctga atctcttctc
     3061 cgggcaaccc tacgtcggtc catccgcgtt cgcgcacaaa ggcggcatgc acgtggcggc
     3121 cgtgcagaag gatgtcagca cgtacgaaca cgtcgacccc gccagcgtcg gcaacacgcg
     3181 gcggattctc gtcagcgagc tgtccggcat ctccaacatc gccaccaagg cgggcgtgaa
     3241 gttcaacatc gaaaatgaca aggcggcgct gaagaacatc cttgagcgcg tgaagacgct
     3301 ggagaacgag ggctatcatt tcgaagccgc cgaggcgagc tttgagctgc tgctgcgaaa
     3361 ggagatcggc cggcatcggt cgttcttcaa cctcgatcat taccgcacgg tagtgctcaa
     3421 gagcggcgaa ggcgtggaag caatctcgga agcgaccgtg aagatgcgcg tgggcgacgt
     3481 ggtcgagcat cgcgtggccg agggcgatgg tccggtgaac gcgctggatc tggcgctgcg
     3541 ccgcgcgctc aagccgcact atccgcagat cgataaggtg cacctgatgg actacaaggt
     3601 gcgggtgatc aattccaacg acgagacggc cgccaaggtg cgggtgatca tcgagtcaaa
     3661 gcgcgagcac gccgacggca gcaaggaaat tttcggcacg atcggggtga gcgggaacgt
     3721 gatcgacgcc agctggcagg cgctggtgga cgcgtatgag tatcacctga tccacgtgat
     3781 ggaagaaacc ggcgcgaagg cgccggcgac gagcgcaccg atctcggagc ccgtcggaca
     3841 gacctaaacc ctgcgcggcg tggtcgcttc agcgaccatg tctttacgcc ttcggacgct
     3901 caagaaacat ggtcgctaaa gcgaccacgc ctgtcgtaca aggacgcgcc acatggacgc
     3961 ttatgacgcg ttgatcacgg agctgcgcga gctcgcgctg ctcggctcga tctcatctgc
     4021 gctcggttgg gatgagcaga cgcacctgcc gcccaaaggc gctgaccatc gcgccaacca
     4081 gatttcctat ctcgctgcca tgcagcatcg gcgctttacg tcgccgcgga ttggcgagct
     4141 gctgcaagcc gtcgaatcat ccgacttggt gaagcattca gacagcgaca tcgccgtgaa
     4201 cgtgcgcgag acgcggcgga attatgacaa ggcgcggaag ctgccggagt cgctggtcga
     4261 ggagatgagc cggcactccg tgctgtcgca gcaggcgtgg accgaggcgc gaaaacgcgc
     4321 ggatttcccc gcgtttgtgc cgtatctgga aaggacgctg cagctgaaga agcaggaggc
     4381 ggcgtgcatc ggttatacga cgcagccgta cgatgcgctg ctcgacgcgt acgagccggg
     4441 tgatacgtcg gcggacatcg cgcgcgtgtt cgagcaactg cgcgggccgc tggtcgatct
     4501 gattcgccgg gtgggtgaat cgcggcgacc ggcgccgccg gtcgaaatca tgcagcgaac
     4561 tttccccgcg gcggcgcagg aagcgttcgc gaaagaggcg gccgcgcgaa tcggtttcga
     4621 cttcgacgcc ggccggctgg acgtcagcgt tcacccgttc tgttccgagc ttggcccgca
     4681 cgatgtgcgc atcaccacgc gctacgatga gcatcagttc aacgacgcgt tcttcggcgt
     4741 gctgcacgag acgggtcacg ggctgtacga catgggcctg ccggccgagc actacggcac
     4801 gccgctcggg tcgttcgtgt cgatgggcat tcacgaaagc cagtcgcgga tgtgggagaa
     4861 cctggtcggc cgcagccggg cgttctggtc gttcttcctt ccgaaggcgc gcgagacgtt
     4921 cagcgaagcg ctcggcggcg tgagcgatga cgactggtat cgcgcgatca acgacatccg
     4981 cccgagcctg atccgcgtgg aagccgacga ggcgacctac aacctgcaca tcctgctgcg
     5041 atttgagatg gagcaggcaa tgatgagcgg caacctgggc gtcggcgatc taccggcggc
     5101 gtggaatgag cggatgaaat cgtacctggg tctgacgccg cccgacgcgg cgacgggcgt
     5161 gttgcaggat atccactggg cgtttggcgg catcggttac ttccccacct acaccctggg
     5221 caacctgtac gcggcccaat tcttcgagca ggctcgaaag gatcttggcg atctggatgc
     5281 gcagttcgcc cgcggggaat ttcgtccgct actcgattgg ctgcgccaga acatccatcg
     5341 tcacggccaa cgatacaaag cgcgcgagct ggtgcggcgc gtgacgggga aggacctgac
     5401 ggcgcagccg ctgctggact atctgaacgg aaaggcgagc gaagtttatg atgtttgatg
     5461 tgcgcctggt ctcctctccc acgtactcgt gggagagggt taaggtgagg gttatttcgg
     5521 aggtcgaacg cctcttgaaa acaaaatcac cctcaccccg gccctctccc acgagtacgt
     5581 gggagagggg gaaagg
//
LOCUS       DATIMH010001160         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_272700, whole genome shotgun sequence.
ACCESSION   DATIMH010001160 DATIMH010000000
VERSION     DATIMH010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272700"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VLI90_19970"
     CDS             complement(<1..742)
                     /locus_tag="VLI90_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16551.1"
                     /translation="MGFASGSVSFRRFAVVGKSPATVDQDMLDKLAEHALRPQEVGVP
                     EEIEYGWSGGRHVLDEQFSFEHNAFAEALFFGLRIDTNKVPGDLKRAYMIMEEDAVAA
                     GNPSGFISKAQKKDVKDTIRQKVDDELRSGKFRRSKLLPLLWDLPSQTLYASATGKRF
                     EMLAELFERTFGLSLIPLSAGSIALRLLEPRGRRRDYEDFRPTRFVYGPEGESQYPEY
                     PWVMKGPEPKDFLGNEFLLWLWHEADHRD"
     gene            953..2407
                     /locus_tag="VLI90_19975"
     CDS             953..2407
                     /locus_tag="VLI90_19975"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSV16552.1"
                     /translation="MAQRSLSAVAAVLLLGLFLAPPARGEGNKAATSSTTEPFPSATP
                     QAVVDVSNKIFPAVVRLDVAQEIYSEGKRNLRRGIGSGVIIDAAGHILTNYHVAGRAA
                     EIFVTLFNKERVHAKLIGDDHWTDLAVVQIDMDEVKQKKITFNFAPLGDSATLITGQD
                     VMAIGTPFGLNRTMTLGIISNNERTFYPERMTIDEYETGEFSNWVQMDTPINPGNSGG
                     PLVDMNGTVIGINTRGGGQNLNFAIPIDTAKPVITAILKSATPDKKGRIDRSDLGLDF
                     KPLQDLETFYDIDINKGVLVNSVDNYSPAQKAGVKSQDILLEINGRPTNVRFPEEIAG
                     VRKMIADLPIGSDVDLLIKRGKESLHIHTQTAKLEGAVGEEKELKTWGVSVRDVTRAY
                     ANDQQLDDDIGVVVTTMSPGYPAAKAELESGDVIRAVNQQPATDLDEFLRLYNESLRN
                     KDARVLVEIQRGRGRRSIVLKVTYTDEASTQPAK"
BASE COUNT          527 a          794 c          686 g          417 t
ORIGIN      
        1 catcgcgatg gtccgcctcg tgccacagcc acagcagaaa ttcgttgcca agaaaatcct
       61 tcggctccgg ccccttcatg acccacgggt attccgggta ttggctctcg ccttccgggc
      121 catagacgaa acgcgtgggt cgaaagtctt cgtaatccct ccgccgcccg cgcggttcga
      181 gcagccgcag cgcgatcgac ccagccgaca acgggatcag gctcaagcca aacgttcgct
      241 cgaacaactc cgccagcatc tcgaagcgct tgcccgtcgc cgacgcgtac agcgtctgcg
      301 acggcagatc ccacaacagc ggcagcagtt tgctgcggcg gaactttccg ctgcgcagct
      361 catcgtccac cttctgccgg atcgtgtcct tcacatcttt cttctgcgcc ttggagatga
      421 agcccgacgg attccccgcg gccaccgcgt cctcctccat gatcatgtag gcccgcttca
      481 gatcgcccgg cactttattc gtgtcgatgc gcaggccgaa gaacagcgcc tcggcgaacg
      541 cgttgtgttc gaagctgaac tgctcgtcca gcacatggcg gccaccggac cagccgtact
      601 caatctcctc cggcacgccc acttcctgcg ggcgcagcgc atgctcggcc agcttgtcga
      661 gcatgtcctg atcgacggtc gcaggagatt tgccgacgac ggcgaagcga cggaacgaaa
      721 cggaacctga agcaaatccc atgaacactc cagtgcaaat tcgatgacga cacctccgac
      781 gccggtgtct gaggtactcc gaggacccgg gtactttcgc gcgcaagaac ccgggtcttc
      841 ggagtacctc agacaccggc gtcgggactc atcatgcatc ctacgccgtt ccacgatttc
      901 tccaacgcaa cgttgcgacc gcgacaacat tcgcgcatgt tatccacact ccatggcgca
      961 aagatcatta tcggcggtgg cggcggtgct tttgctcggt cttttcctcg cgccacccgc
     1021 gcgcggcgaa ggaaacaagg ccgcaacttc cagcacgacc gagcccttcc ccagcgccac
     1081 accacaggcg gtggttgatg tcagcaacaa aattttcccg gccgtcgttc ggctcgacgt
     1141 tgcccaggag atttactccg aaggcaagcg caaccttcgc cgcgggatcg gctccggcgt
     1201 catcatcgac gccgcggggc acatcctgac gaactatcac gtcgccggtc gggcggccga
     1261 gattttcgtc acgctgttca acaaggagcg cgtgcacgcg aagctgatcg gagacgatca
     1321 ctggacggac ctggcggtcg tgcagatcga catggacgag gtgaagcaga agaagatcac
     1381 cttcaacttc gcgccgctgg gcgacagcgc cacgctcatc accgggcagg acgtgatggc
     1441 catcggcaca cccttcgggc tcaatcgcac gatgacgctc ggcatcatct ccaacaacga
     1501 gcgcacgttt taccccgagc gcatgacgat cgacgaatac gagactggcg agttttccaa
     1561 ctgggtgcag atggacacgc cgatcaaccc cggcaacagc ggcggtccgc tggtggacat
     1621 gaacggcacc gtcatcggca tcaacacccg cggcggtggg cagaatttga acttcgccat
     1681 tccaatcgac acggccaaac cggtcatcac cgccattttg aaatcggcga cgcccgacaa
     1741 gaaaggccgg atcgaccgca gtgatctagg cctcgacttc aaaccgctgc aggacctgga
     1801 aactttttac gacatcgaca tcaacaaggg cgtgctcgtc aacagcgtcg ataattattc
     1861 gccggcgcaa aaggccggcg tgaaatccca ggacatcctg ctggagatca acggccggcc
     1921 gaccaacgtg cgctttcccg aagaaatcgc cggcgtccgg aagatgatcg ccgacctgcc
     1981 gatcggcagc gatgtcgatc tgctgatcaa gcgcggcaag gaatcgctgc acattcacac
     2041 gcagaccgcg aagctggaag gcgcggtggg cgaagagaaa gaactcaaaa cctggggcgt
     2101 gagcgtacgc gacgtcaccc gggcatatgc caacgatcaa cagctggacg atgacattgg
     2161 cgtggtcgtc accacgatga gccccggcta ccccgcggcg aaagcggaat tagaatcggg
     2221 cgatgtcatc cgcgccgtca accaacagcc ggcgacggat ctggatgagt tcctccgcct
     2281 atacaacgag tcgctgcgca acaaagacgc ccgcgtgctg gtggaaatcc aacgcggccg
     2341 cggccggcgg tcgatcgtgc tgaaggtcac gtacaccgac gaagcgtcca ctcaaccggc
     2401 gaaatgattt tttagcgcgg gcgt
//
LOCUS       DATIMH010001161         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4024384, whole genome shotgun sequence.
ACCESSION   DATIMH010001161 DATIMH010000000
VERSION     DATIMH010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4024384"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VLI90_19980"
     CDS             <1..842
                     /locus_tag="VLI90_19980"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HSV16553.1"
                     /translation="KLAKAAQRFVGEPVRVTMAPEALAREDGFAIGFVGPHAILGRND
                     TFMLVDPDAAQGGFWASGANEVDHHVKHFNWAREVGDVLADPTKVAIADIRNAAAGDP
                     SPKNDGGKLLASKGIEIGHVFKLGTKYSDALGANYLDDKGAQHPLIMGCYGIGVGRIL
                     IAAVESLHDDKGIIWPAAIAPFSVIVTPIKYEGEVKAAADKLYDQLNAAGIDTLLDDR
                     PDARPGVKFADADLIGIPVRITVGDRGLNEGKVELKRRTAADVEMVETQQVIERVKAL
                     LAR"
     gene            893..>2234
                     /locus_tag="VLI90_19985"
     CDS             893..>2234
                     /locus_tag="VLI90_19985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV16554.1"
                     /translation="MTLPTPATSATDAQSSRTFILRVWLFIILLVAVLLRLINLSAQS
                     FWLDELWTIEVSAGQASEHRSLPVDVLLTAPPDLTHAAGLHGFTRIWTGMDQIIGPPL
                     YHIALRVWMFAFGESESAIRSFSALCSSVAVFLLFFCRRPGDPPQAALWGCAVMAVAG
                     PQIVYAQETRPYAMMLALGLAAVVLVQRVERLGPTAGRLAALWFVLLCLLLTHYFAVG
                     APLAVMLYAAAQTRGRTRIALLLTAAAAGLCFAVCWLPFMSRQVPQFSVTDRAAAFLA
                     EQSPNHLVRTGWRLATLPISLLCDRRPIPMYYASGLLIYLAAAVLAWRRRIWSIWPVW
                     FAGIVAVPGVLDLLRATRHLEYIRYSLLAGPAVYIVIAHLFFESPRFRFMAHIVPAVV
                     VLCCVLQLRRAYDTQKPDYRAAAAVLRASASGGDVIVFAGPRKSYAPQVLYLGIS"
BASE COUNT          352 a          674 c          759 g          449 t
ORIGIN      
        1 cgaaactggc gaaggcggcc cagcgattcg tgggcgaacc ggtgcgcgtg acgatggcgc
       61 ctgaggcgct ggcgcgcgaa gatggcttcg cgatcggctt cgtcggtccg cacgccatcc
      121 tgggccgcaa cgacaccttc atgctggtcg atcccgacgc cgcccagggc ggattctggg
      181 ccagcggcgc gaacgaagtc gatcatcacg tcaagcattt caactgggcg cgcgaggtgg
      241 gcgatgtgct ggccgatccg acgaaggtcg cgatcgccga catccgcaac gccgccgccg
      301 gcgatccgtc gccgaagaat gacggcggaa aacttctcgc atccaagggc atcgagatcg
      361 gccacgtctt caagctcggc acgaaatact ccgacgcgct gggcgcgaat tacctggacg
      421 acaaaggcgc acagcacccg ctgatcatgg gctgctacgg catcggcgtc ggtcgcattc
      481 tgatcgccgc cgtcgaaagc ctgcacgatg acaagggcat catctggccg gcggccatcg
      541 cgccgttcag cgtgatcgtc acgccgatca agtacgaagg cgaagtgaaa gccgccgccg
      601 acaagctgta cgaccagctg aacgccgccg gcatcgacac gctgctggac gaccgccccg
      661 acgcccggcc gggcgtgaag ttcgccgatg cggatttgat cggcatcccc gtgcgaatca
      721 ccgtcggcga tcgcgggttg aacgaaggca aggtcgagct gaaacgccgg accgcggccg
      781 atgtggagat ggtcgagact cagcaggtga tcgagcgcgt caaagcgctg ttggcccgct
      841 gattcgcatt ggatgggctg cgatgttcgt gcgcacgtcg gagatgtgaa cgatgacatt
      901 gccgacaccg gccacgtcag cgacggacgc gcagtcatcg cgaacattca tccttcgggt
      961 gtggctcttc atcatcctgt tggttgcggt gctgctgcga ctgatcaacc tttccgctca
     1021 aagtttttgg ctcgacgaac tgtggacaat cgaagtgtcc gccgggcagg cgtcggagca
     1081 tcgcagcctg ccggtggatg tgctgctgac ggcaccgccg gatttgacgc acgccgcggg
     1141 gctgcatggg ttcacgcgca tctggaccgg aatggaccag atcatcggcc cgccgctgta
     1201 tcacattgcg ttgcgggtct ggatgttcgc gttcggcgag agtgaatcgg cgattcgatc
     1261 gttctccgct ctttgttcaa gcgtagccgt gtttctgctg tttttctgcc ggcggccggg
     1321 cgatccaccg caggcggcgc tgtgggggtg cgccgtcatg gccgtcgcgg ggccgcagat
     1381 cgtctatgca caggagacca ggccgtatgc gatgatgctc gcgctcggat tagccgccgt
     1441 cgttcttgtg cagcgggttg aacgactggg gccgactgcc gggcggctcg cggcgctgtg
     1501 gttcgtgtta ctgtgtttac tattgacgca ttattttgcc gttggcgcgc cattggcggt
     1561 gatgttgtac gcggcggcgc agacgcgcgg caggacgcgc atcgccctgc tgctaacggc
     1621 cgcggcggcg gggctttgct tcgcggtctg ctggctgccg ttcatgtcgc ggcaggttcc
     1681 gcagttttcg gtgaccgatc gggcggccgc gtttttggcg gagcagtcgc cgaaccatct
     1741 ggtgcggacg ggatggcgac tggcgacgct gcccatctcg ctgttgtgcg accgccggcc
     1801 gatcccgatg tattacgcga gtggattgct gatctatctc gcggcagcag tgctggcgtg
     1861 gcggcggcgc atctggtcga tctggcccgt ttggtttgcc gggattgtgg ccgtgcctgg
     1921 ggtactcgat ctgctgcgcg ccacgcggca cctggaatat atccggtatt cactgctggc
     1981 cggcccggcg gtgtacatcg tcatcgctca tttgtttttc gaatcgccgc gatttcgatt
     2041 catggcgcac atcgttcccg ccgtcgtcgt cctgtgctgt gtgctgcagc tgcgccgggc
     2101 gtacgacaca cagaagccgg actatcgggc ggcggcggcc gtgcttcggg catcggccag
     2161 cgggggtgac gtgatcgtct tcgccggtcc acgaaaatca tatgcgccgc aggtcttgta
     2221 cttggggatc agcc
//
LOCUS       DATIMH010001162         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1433721, whole genome shotgun sequence.
ACCESSION   DATIMH010001162 DATIMH010000000
VERSION     DATIMH010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433721"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2831
                     /locus_tag="VLI90_19990"
     CDS             <1..>2831
                     /locus_tag="VLI90_19990"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HSV16555.1"
                     /translation="QFTRDAIANLPAGDAASLRSFVETTPGSFTFDDTSATLYVNLGS
                     ALAGHTITAAVREDAVFSNGQSFVTFQDLAASETAKMDAGYTFRIEKGNDVSVIDCDA
                     SLAGKHHFGDIDSTHFLGRGLTADQSSPDLGFGGASAFVSFSDAINGRQNDTSQWIDC
                     IYTHANGAYPSFISHGDPGAIGSVLLQNITDMNGYGSGITVYRTSDTERVTINGGNIG
                     GGFVQVDTNNSVINGLHVTGQMGSITINGTGTVVQNCLLNGIQPFPAGGFKGGIIDGG
                     HNNTIRFNTIQISVPNGPGITVENDDSNTQIYGNIIDAPQPIRLLFNSGNGAVSSDFN
                     VFGTTSAAAPVIGLGDVNQSESEPLQNWTSLGRDTHSPTGDPQFTSAAAGDFSVQQTS
                     PAVGLYTATPDTSVTTIDTDLLGHARPAGARDAGALEFVPVVAQVSAATTSVQEPATG
                     ATAFATFTVTLSQSPSAAVTVNYATSDGTAIAGADYDAATGALTFAPGETSKTINVTV
                     HGVAGTTGDKTFNLNLSNPSANATIATQQVPATITATPGGGTVVPVAFTARTPLAYND
                     SAGHAVKVLLIGPGSGEASFDPGAAEPSLITLANTTAASLLVVRSTGGATTLHDVDVQ
                     SPIGIIAGPSANLAGNLTLSSGARIVLLGDASGQHTINIGASVTPVTLRLGNVSDLSV
                     NSSAAIAAIFASNWIDTNATADTIAAPSIAAISITGAFGAGVSVSGSVNSARVVGAIT
                     GGAWNIGGGGRALVAGSTAPTWSATYAGDLGVASILGNFAGSLTASTLRAMVVRGDLS
                     GNVHLTNSTTVQPALGTLNVVGSINGADVRSAGNISVVSAASLVNSVLFAGVNDAMAT
                     LPSDTSAFTAPASILLLNVRGAPGVDASFSNSLVSASSIRTAVVHGMHGDAAATPFGI
                     STNQMSIFVDIHPPTAPIVWRSTTGKGTPT"
BASE COUNT          517 a          959 c          908 g          447 t
ORIGIN      
        1 caatttacgc gcgatgcgat cgcgaacctg ccggccggcg atgccgccag cctgcgcagc
       61 tttgtcgaga cgacgccggg cagtttcacg tttgacgaca cctccgccac gctctatgtc
      121 aacctcggtt ctgcgcttgc cggtcacacc atcactgccg ccgtgcgcga ggacgcggtc
      181 ttcagcaacg gccagagctt cgtcacgttc caggatttgg cggcatcgga aaccgcgaaa
      241 atggatgcgg gatacacctt ccgcatcgag aaggggaacg atgtctcggt gatcgactgc
      301 gatgcatcgc tggcgggcaa gcatcatttt ggcgatatcg attcgacgca tttcctgggg
      361 cgtgggttga cggcggatca atcatcgccg gacctgggat tcggcggggc gtcggcgttc
      421 gtcagcttca gtgacgcaat caacggccgg cagaacgaca cgtcgcagtg gatcgactgc
      481 atctacacgc acgccaacgg cgcgtatcca tcgttcatct cgcacggcga ccccggcgca
      541 atcggcagcg tgctgctgca gaacatcacc gacatgaacg gctacggctc aggcatcacg
      601 gtctatcgca ccagcgacac cgaacgcgtc accatcaacg gcgggaacat cggcgggggg
      661 ttcgtgcagg tggacacgaa taattcggtc atcaacggtc tgcacgtgac gggccaaatg
      721 ggctcgatca cgatcaacgg caccggcacg gtggtgcaga actgcctgct gaacggcatc
      781 cagccgttcc cggcgggggg gttcaagggc ggcatcatcg acggggggca caacaacacg
      841 atccgattca acacgatcca gatcagcgtc ccgaacgggc ccggcatcac ggtcgaaaac
      901 gacgacagca acacgcagat ttacggcaac atcattgatg cgccacagcc gatccgcctg
      961 ctgttcaaca gcggcaacgg ggccgtgagc agcgacttca acgtcttcgg cacgacgtcg
     1021 gccgccgcgc cggtgatcgg gctgggggac gtgaatcaaa gcgagtccga gccgttgcag
     1081 aactggacat cgctggggcg cgacacgcat tcgccgacgg gtgacccgca gttcaccagc
     1141 gccgccgcgg gggatttttc ggtccagcaa acatcgcccg ccgtgggcct gtacacggcc
     1201 acgccggata cgagcgttac gacgatcgac acggatctcc tcggtcacgc ccgcccggcc
     1261 ggggcgcgcg acgccggagc attggaattc gttcccgtgg tcgcgcaggt gagtgcggcg
     1321 acaacgtcgg tgcaggaacc ggcgacgggc gcgacggcat tcgcaacgtt caccgtgacg
     1381 ttgagccagt cgccctcggc agcggtgacg gtgaattacg cgaccagcga tggcaccgcc
     1441 atcgccggcg ccgattatga cgccgccacc ggtgcgctca cgttcgcgcc cggcgagacg
     1501 agcaagacga tcaacgtcac cgtgcacggc gtggccggca cgacgggcga taagacgttc
     1561 aacctgaacc tctcaaaccc cagcgccaac gcgacgatcg caacgcagca ggtgccggcg
     1621 acgatcaccg caacccccgg cggcggcacc gtggtgccgg tcgcgttcac tgcgcgaacg
     1681 ccgctggcgt acaacgattc cgctggacac gcggtgaagg tcctgctgat cgggcccggt
     1741 tcgggcgaag cgtcgttcga cccgggggcg gccgagcctt cactgatcac gctggcgaac
     1801 acgacggcag cctcgctgct ggtcgtgcga tcgaccggcg gggcgacgac gctgcacgat
     1861 gtggatgttc aaagcccgat cggcatcatc gccggtccct ccgcgaacct cgcgggcaac
     1921 ctcacactga gcagcggggc gcgtatcgtc ctgctgggcg atgcttcggg tcagcacacg
     1981 atcaacatcg gcgcttcggt gacgccggtc acgctgcggc tggggaacgt gagcgacttg
     2041 tccgtcaact cctcggctgc gatcgcggcg atcttcgcgt cgaactggat cgataccaac
     2101 gcgacggcgg acacgattgc cgcgccgtcg atcgccgcga tcagcatcac cggcgcattc
     2161 ggcgccggcg tctccgtcag cggtagcgtc aacagcgccc gcgtcgtcgg cgcgatcacc
     2221 ggtggcgcat ggaacatcgg cggcggtggg cgcgccctag tcgcgggttc gaccgcgccg
     2281 acgtggtccg ccacgtacgc tggcgacctg ggcgtcgcca gcatcctcgg caactttgcc
     2341 ggctcgctca cggccagcac gctccgcgcg atggtcgtgc gcggcgatct gagcgggaac
     2401 gtgcacctga cgaattcgac caccgtacag ccggcgcttg gcacgctgaa cgtcgtcggc
     2461 tccatcaacg gcgccgacgt gcgcagcgcc ggcaacatct ccgtcgtgag cgccgcctcg
     2521 ctcgtcaact ccgtgctatt cgccggcgtg aacgatgcga tggcgacgct gccgagcgac
     2581 accagcgcgt tcaccgcgcc ggcgtcgatc ctgctactaa acgtccgcgg cgcgcccggc
     2641 gtcgatgcgt cgttctcaaa ttcgctggtg tccgcctcga gcatccgcac cgccgtcgtc
     2701 cacggcatgc acggcgacgc cgcggcgacg cccttcggca tttcgacgaa tcagatgagc
     2761 atcttcgtcg atatccatcc accaacagcg ccgatcgtct ggcgatcgac caccggcaag
     2821 ggcacgccga c
//
LOCUS       DATIMH010001163         8365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4320152, whole genome shotgun sequence.
ACCESSION   DATIMH010001163 DATIMH010000000
VERSION     DATIMH010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4320152"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..923)
                     /locus_tag="VLI90_19995"
     CDS             complement(138..923)
                     /locus_tag="VLI90_19995"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HSV16556.1"
                     /translation="MSDTQVKPDRMAWIKGPVANCSVFPDKPVRAWRLVLLGAPGVGK
                     GTQAELLAERLGACHLSTGDVFRAAKAVPECQCSPSMAGALDYMRRGELVPDHAVLSI
                     IKERIGCLRCRGGFLLDGFPRTVGQAEVLQMFLRAYDVLPDAVINYDLPTSEIITRIS
                     GRLTCADCKAVFHATSRSPKQAGVCDECGGKLFQREDDRPETVKVRLEAYRQQTMPLI
                     NYYKKLGLLVTVDAWGTPADVFAKTIETLRSSTLAGHQLSFDS"
     gene            complement(1080..1256)
                     /locus_tag="VLI90_20000"
     CDS             complement(1080..1256)
                     /locus_tag="VLI90_20000"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSV16557.1"
                     /translation="MAAIVDVEKCDGCHSCEEQCPTDAIKVTENVAVVSVDDCIDCNA
                     CEDACPTQAVKVQS"
     gene            complement(1321..2352)
                     /locus_tag="VLI90_20005"
     CDS             complement(1321..2352)
                     /locus_tag="VLI90_20005"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HSV16558.1"
                     /translation="MSLATKYLGLNLAHPFMPGASPLVDSLDTVRRLEDAGASAIVMH
                     SLFEEQISREQHATIYHMEMYAGAFAEVSSYFPKPSEFALGPDAYLKHIAKIKQAVGV
                     PVIASLNGTTRGGWTRYAELLHQAGADALELNLYYLPTDASETGGTVERRVVDVLSSV
                     RSVVGIPVAVKLSPFYSALPDLVAQLDDAGADGLVLFNRFYQPDIDLENLEVVPKVEL
                     STSQELLLRLRWLSILSPHTDMSFACSGGVHTPEDAIKALMCGAHAVQLVSCLLKHGV
                     GYLKAVRQGVQDWMDRHEYASLTELHGSMNLSRCPDPTAFERANYMRTLQSWPPRDML
                     AAAGTDVAK"
     gene            complement(2490..6095)
                     /gene="nifJ"
                     /locus_tag="VLI90_20010"
     CDS             complement(2490..6095)
                     /gene="nifJ"
                     /locus_tag="VLI90_20010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HSV16559.1"
                     /translation="MTARNQPQQRKMVTLDANEAAASVAHRLSEVICIYPITPSSPMG
                     EFSDEWSAKGMTNIWGTVPQVTEMQSEAGAAGAVHGALQAGALSTTFTASQGLLLMIP
                     NMYKIAGELTPFAMHVTARTLATHALSIFGDHSDVYACRQTGFAMLCSNSVQEAHDLA
                     LVAHAATLESRVPFLHFFDGFRTSHEVSKIEQLNDDDLRALVDDQLVAAHRGRALSPD
                     HPVIRGTAQNPDAFFQAREACNKFYQVCPTIVHETMRKFAALTGRSYDLFDYYGDPNA
                     DRVIVAMGSGCETIEQTIDYLRGRGEKLGLIKVRLYRPFPVNAFVAALPKTTVRLAVL
                     DRTKEPGAVGDPLYLDVVTALLEAQFGGFLPMHSMPRIVAGRYGLSSKEFTPAMVAGI
                     FDELNKTEPKNHFTVGIVDDVTHTSLPWDDQLDIEAADVTRAVFYGLGADGTVGANKN
                     SIKIIGEETDNWAQGYFVYDSKKSGAMTTSHLRFGPRPIKAPYLIKRAGFVAVHQWKF
                     LEKMDVLELAGDSAVVLLNAPFGPGAVWDQLPIEIQQPIIDRKLKLFVIDAFKVARDT
                     GMGVRINTIMQTCFFALSGVLPREEAIAQIKKSIKKTYGRKGDEVVQKNFAAVDHTLA
                     HLYEVKVPATASSTFHQESIVSGDAPDFVKRVEGMMLANKGDLLPVSAFPVDGSWPLG
                     TARYEKRNIAMDIPVWESGICIQCNKCVMACPHAAIRAKFYDPAALGGAPDSFKAVDF
                     KSPDQRGKKYTLQVAPEDCTGCTVCVAVCPAKNKSNPREKAINMRPQMPIRDAERSNY
                     DFFLNIPEADRRTIKLDVKGSQFLQPLFEYSGACSGCGETPYVKLMTQLFGDRAVIGN
                     ATGCSSIYGGNLPTTPYCTNHDGRGPAWANSLFEDNAEFGMGMRLALDKHSEQAKELL
                     QKISSAIGDTLVTEILTANQSDETGIASQRQRIVALREKLARVPSALLPQAKRLEALA
                     DYLVKKSVWIVGGDGWAYDIGFGGLDHVLASGRNVNILVLDTEVYSNTGGQQSKATPI
                     GAAAKFASAGKTSGKKDLGLIAMSYGNAYVARVAFGAKDVQTVKAFTEADSYDGPSII
                     IAYSHCIAHGYDLANGPEQQKIAVETGYWPLYRFDPRRVAAGEAAMQLDSNPPKGHPT
                     QFTKNETRFRMVEQQNAERFQAMMETAKRDIDARYALYAQLATVKPANGENGIGH"
     gene            complement(6159..7229)
                     /locus_tag="VLI90_20015"
     CDS             complement(6159..7229)
                     /locus_tag="VLI90_20015"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16560.1"
                     /translation="MSDHALNDLDERLLQAVQEEVPLVPRPYKALADHVGASEQDVIE
                     RLSALRWGTRAVIRQISAIFDSKSLGYQSTLVAARIPAEQLDAAAAVISAHPGVSHNY
                     RRDHAYNLWYTLAVPPDSRLGLQRTARILHQQSGALATRLLPTLKMYKIGVKLDLGGN
                     AGAAKPEAVKHQPVEASDAEPLTEMDKDLIRALQQDLPIQTRPFDLLAAQAGISTETL
                     LEAGTQFIRDKRMRRFSAVLRHRELGFDANAMGVWDVPPEECARFGKIAACFPAVSHC
                     YLRPTYEDWPYSIFTMVHARTREQGNAVLHGIAEATGIARYTALYSTHEYKKARIRYF
                     TGDIEAWEAAHAAAAEAEVLSR"
     gene            complement(7258..8220)
                     /gene="hemC"
                     /locus_tag="VLI90_20020"
     CDS             complement(7258..8220)
                     /gene="hemC"
                     /locus_tag="VLI90_20020"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HSV16561.1"
                     /translation="MTKQSPTSVLRLGTRGSQLARTQSAMVAEQLSRRCPGLSVELVI
                     YKTSGDIFKDRPLHEEGGKGLFTKELELALLAGEIDFAVHSLKDVPVTMPLVAQDNLV
                     LAAIPLREDPRDVLVSSIAHRVMDLPAAARVGTGSLRRRCQLLAARPDLEIVPLRGNL
                     DTRLRKSRDGEYDAIVAALAGLKRACLFDAATMAAIPTEEMLPAAGQGALALQCCRDD
                     ERTRGVLSLLHDPITARCVRAERAIVTGLKGDCHSPIGAFATIESEELTLRVAVGAPG
                     GRLPVLTAAATAPARRWQAAVNQVLYELRSMDADDLMDHMAAAI"
BASE COUNT         1386 a         2689 c         2644 g         1646 t
ORIGIN      
        1 cgtgcttcac caggatggcc actgcaaagg aatgaaaagc cctgttcgga aggatcttga
       61 ttgagccgct tgcggcttcg cagaccaagc gatgtggttt gcgaagccgc aatctgttgc
      121 gcaggggcgc gccggcatca gctgtcgaag gacagctggt gccccgcgag cgtcgatgac
      181 ctgagcgtct cgatcgtctt cgcgaacaca tccgccggtg taccccacgc gtcaaccgtt
      241 accaggaggc cgagcttctt gtagtagttg atcagcggca tcgtctgctg gcgatacgcc
      301 tccaggcgca ccttgactgt ttcgggacgg tcgtcttcgc gctggaagag cttcccgccg
      361 cactcgtcgc agacgccggc ttgtttcggc gaacgcgagg tcgcgtggaa gacagctttg
      421 cagtcggcgc aggtgagtcg tccgctaatg cgggtgatga tctcgctggt cggcagatcg
      481 taattgatga ccgcgtccgg cagcacatca tatgcccgca ggaacatctg cagcacctcg
      541 gcctggccga ccgtgcgggg gaacccgtcg agcaggaacc caccgcggca gcggaggcaa
      601 ccgatgcgct ccttgatgat ggacaacacc gcgtggtccg gcaccagttc accgcggcgc
      661 atgtagtcca atgcgccggc catcgagggg ctgcattggc attcgggcac ggccttggcg
      721 gcgcgaaaga cgtcgccggt cgatagatgg caggcgccca ggcgctcggc cagcagttcg
      781 gcctgcgtgc ccttcccgac gccgggcgcg cccagcagga ccagccgcca cgcccgcaca
      841 ggcttgtcgg ggaatacact acagttggcg accggacctt tgatccacgc cattcgatcc
      901 ggtttgactt gtgtgtctga catgatgaac tcgcagtgga gggattgaat gcggtgctcg
      961 cgcgatcctg tccttgctcg cgcgatcgcc atttatcgaa tcgcggatat ctacatccgt
     1021 cggcgctgga cgctcgctcg gccctgcggg ccgccgctaa aaggggcgtt cgaaacgccc
     1081 tagctctgca ccttcaccgc ctgcgtgggg caggcgtctt cgcaggcgtt acagtcgata
     1141 cagtcgtcca ctgacaccac cgcgacgttc tccgtcactt tgatggcatc cgtcgggcac
     1201 tgctcttcac acgagtggca tccatcacat ttctcaacgt caacaatcgc agccatgggc
     1261 gtctcctatt ctcaagacct ggcgtaagcc tggcggcttt taatcgcggg tcgttagtct
     1321 tcatttcgca acatccgtgc ccgcagcggc gagcatgtcg cgcggcggcc agctttgcag
     1381 cgtccgcata tagttcgccc gctcgaacgc ggtggggtcc gggcagcgcg acaggttcat
     1441 gcttccgtgc agctcggtca gggacgcgta ttcgtgccga tccatccaat cctgcacgcc
     1501 ctggcggacc gccttcagat agccaacacc gtgcttcagc aggcacgaca ccagctgcac
     1561 cgcgtgggcg ccgcacatga gggctttgat ggcgtcttcc ggcgtgtgca cgccgccgct
     1621 gcaggcgaag gacatgtccg tgtgcggcga gaggatcgat agccatcgta atcggagcag
     1681 cagctcttgt gatgtcgata gctccacctt tggcacgact tccaggttct ccaggtcaat
     1741 gtccggttga tagaaccggt tgaacagcac gagcccgtcg gcgccggcgt cgtccaactg
     1801 ggcgaccagg tccggcagtg ccgaatagaa cggcgacagc ttcacggcaa ccgggatgcc
     1861 aacgacgctg cggacgctgc tcagcacgtc caccacgcgc cgctcgaccg tgccgccggt
     1921 ctccgatgca tcggtcggca ggtagtacag gttcagttcc agcgcatcag cgccggcttg
     1981 atggaggagt tccgcatagc gcgtccaccc gccgcgggtc gtgccgttca gcgacgcgat
     2041 caccggcacg cccaccgcct gcttgatctt ggcgatgtgc ttgaggtacg catcgggtcc
     2101 cagcgcgaac tcagacggtt tggggaaata gctgctcacc tcggcgaacg cgccggcgta
     2161 catctccatg tggtagatcg tcgcgtgctg ctcccggctg atctgctctt cgaacagcga
     2221 gtgcatcacg atggccgacg ccccggcgtc ttccaggcga cgcacggtgt cgagcgaatc
     2281 gaccagcggc gaggcgcccg gcatgaacgg gtgggcgaga ttaagtccaa ggtatttggt
     2341 tgcgaggctc atggtcagtt ccttttcccc ctctcccggt acgccgggag agggcagggg
     2401 tgagggtgat tttgagtgcc agcagctttc caattctcaa atcaccctca ccctaaccct
     2461 ctcccggcgt accgggagag gggaccagat caatgtccga ttccgttctc tccattcgcc
     2521 ggcttcacgg tggccagctg ggcgtatagc gcgtatcgcg catcgatgtc gcgcttggcg
     2581 gtttccatca tcgcctggaa gcgctcggcg ttttgctgct cgaccatgcg gaaacgcgtc
     2641 tcgttcttgg tgaactgcgt gggatggccc ttgggcggat tcgagtcgag ctgcatcgcc
     2701 gcctcgcccg cggcaacgcg ccggggatcg aagcgataga gcggccaata gccggtttcg
     2761 accgcgatct tctgctgctc gggtccattg gccagatcgt agccgtgcgc gatgcagtgg
     2821 ctgtacgcga tgatgatcga ggggccgtcg tagctgtcgg cttcggtgaa cgccttgacg
     2881 gtctgaacgt ccttggcgcc gaaggcgacg cgggcgacgt acgcgttgcc gtagctcatc
     2941 gcgatcagcc ccaggtcctt cttgcccgat gtcttgccgg ccgaggcgaa cttagcggca
     3001 gcgccgatgg gggtcgcctt gctctgctgc ccgccggtgt tgctgtagac ctccgtgtcg
     3061 agcacgagga tgttgacgtt gcgaccggac gcgagcacgt gatcgagccc gccgaaaccg
     3121 atgtcgtatg cccagccgtc accaccgacg atccagacgc tcttcttcac aagataatcc
     3181 gccagcgctt cgagccgctt ggcttgcggt aacaacgcgc tcggcacgcg cgccagcttc
     3241 tcacgcagcg cgacgatgcg ctgccgctgg gaggcgatgc cggtttcgtc gctctggttt
     3301 gccgtgagga tctcggtgac gagcgtgtca ccgatggcgc tggatatctt ctgcagcagt
     3361 tccttcgcct gttcgctgtg cttgtcgagc gccagccgca tgcccatgcc gaattcggcg
     3421 ttgtcttcga acagcgagtt cgcccacgcc ggtccgcgac catcatggtt cgtgcagtat
     3481 ggcgtcgtcg gcaggttgcc gccgtagatc gacgagcaac ccgtcgcgtt gccaatgacg
     3541 gcgcggtcgc cgaagagctg ggtcatcagc ttcacgtacg gcgtttcacc gcagccgctg
     3601 caggcgccgc tgtattcgaa cagcggttgc aggaattggc tgcccttcac gtccagcttg
     3661 atcgtccggc gatcggcctc ggggatgttg aggaagaaat cgtaattgga gcgctcggcg
     3721 tcgcggatcg gcatctgcgg ccgcatgttg atcgccttct cgcgcgggtt cgacttattc
     3781 ttcgccgggc agacggcgac gcacacggtg caaccagtgc aatcttccgg cgccacctgc
     3841 agcgtgtatt ttttgcccct ctggtcgggc gatttgaagt caaccgcctt gaagctgtct
     3901 ggagcgcctc ccaacgccgc cgggtcgtaa aacttcgcgc ggatcgccgc gtgcggacag
     3961 gccatcacgc acttgttgca ctggatgcag atgccggatt cccacaccgg gatatccatc
     4021 gcgatgttgc gcttctcata tcgcgcggtg cccagcggcc aggaaccatc gacggggaac
     4081 gcgctgaccg gcagcaggtc gcccttgttt gccagcatca tcccttcgac gcgcttgacg
     4141 aaatccggcg catcaccgct gacgatcgac tcctggtgga acgtgctcga cgctgtcgcc
     4201 gggaccttca cttcgtacag gtgcgccagc gtgtgatcga ccgcggcgaa atttttctgg
     4261 acgacttcat cgcccttgcg gccgtaggtt ttcttgatcg acttcttgat ctgtgcgatc
     4321 gcctcttcgc gcggcagcac gccggatagc gcgaagaagc aggtttgcat gatcgtgttg
     4381 atgcgcacgc ccatgccggt gtcgcgcgcg actttgaacg cgtcgatcac gaacagctta
     4441 agcttgcggt cgatgatcgg ttgctggatc tcgatcggca gctgatccca cacggccccg
     4501 gggccgaagg gagcattcag caacaccacc gcgctgtcgc cggccagttc cagcacgtcc
     4561 atcttttcga ggaacttcca ctggtgcacc gcgacgaagc cggcgcgctt gatcaggtac
     4621 ggcgccttga tcggccgcgg gccgaaacga aggtgcgacg tggtcatcgc gccgctcttc
     4681 ttcgagtcgt agacgaaata tccctgggcc cagttgtccg tttcttcgcc gatgatcttg
     4741 atcgagttct tgttcgcccc gaccgtgccg tcggcgccaa gaccatagaa gaccgcgcgc
     4801 gtcacgtcgg cagcttcaat gtcgagctga tcgtcccacg gcaacgacgt gtgcgtcacg
     4861 tcatcgacga tgccgacagt gaaatggttc ttcggctcgg tcttgttcaa ctcatcgaag
     4921 atgccggcga ccatcgcggg cgtgaattcc ttgctcgaca aaccgtagcg accggcaacg
     4981 atgcgcggca tcgaatgcat cggcaggaag cccccgaact gcgcttcgag caacgccgtc
     5041 accacgtcca gatacagcgg atcgcccacc gcgcccggtt ccttcgtgcg atccagcacc
     5101 gccaggcgca cggtcgtctt cggtagcgcc gcgacaaatg cgttcaccgg gaacgggcga
     5161 tacaggcgga ccttgatcag cccaagtttt tcgccgcggc cgcgcaggta gtcgatcgtc
     5221 tgctcgatcg tctcgcaacc ggagcccatg gcgacgatca cgcggtcggc gttcggatcg
     5281 ccgtagtagt cgaacaggtc gtacgaccgg ccggtgaggg cggcgaactt ccgcatcgtc
     5341 tcgtgaacga tcgtcgggca gacttgatag aacttgttgc aggcctcgcg cgcctggaag
     5401 aaggcgtcgg ggttctgcgc ggtgccgcga atcaccgggt gatccggcga gagcgcccgg
     5461 ccacgatggg cggcgacgag ttgatcgtcc accagcgcgc gcaggtcgtc gtcgttcagc
     5521 tgctcgatct tcgacacttc atggctggtg cggaagccgt cgaagaagtg caggaacggc
     5581 acgcgcgatt ccagtgtggc ggcgtgggcg accagcgcca ggtcatgggc ctcctgcacg
     5641 ctgttcgaac acagcatggc aaaaccagtc tgccggcagg cgtagacgtc gctgtgatcg
     5701 ccaaagattg acagcgcgtg cgtcgccagc gtgcgggccg tcacgtgcat ggcgaacggc
     5761 gtcagctcgc cggcgatctt gtacatgttc gggatcatca gcaacaggcc ctggctggca
     5821 gtgaacgtgg tgctcagcgc ccccgcctgc agcgccccat gcaccgcgcc ggcggcaccg
     5881 gcttcggatt gcatttccgt cacctgcggc acggtgcccc agatgttggt catgcccttg
     5941 gctgaccact catccgaaaa ttcgcccatc gggctcgatg gggtgatcgg gtaaatgcag
     6001 atgacttccg acaagcgatg ggccaccgaa gcggcggctt cgttcgcgtc gagcgtaacc
     6061 atcttgcgtt gctgcggttg attgcgggcg gtcatttcag gtactccatt tacgatcgat
     6121 agatcgaatt atcctttatc gttgtacaag cgtgccggct accgggaaag cacttctgct
     6181 tcggcggcgg cggcgtgtgc ggcttcccac gcctcgatgt cgccggtgaa atagcggatg
     6241 cgggctttct tgtattcgtg cgtcgagtaa agcgcggtgt atcgcgcgat gcccgtcgct
     6301 tcagcaatcc cgtgcagcac cgcgttgcct tgttcgcgcg tgcgggcgtg gaccatcgtg
     6361 aagattgagt acggccaatc ctcgtacgtc ggccgcagat aacagtggct gacggcgggg
     6421 aagcacgcgg cgatcttgcc gaagcgggcg cattcctccg gtggcacgtc ccatacgccc
     6481 atggcgttgg catcgaaacc aagttcacgg tggcgcagca cagcgctgaa ccgccgcatg
     6541 cgcttatcgc ggatgaattg cgttcccgct tcgagcaacg tctcagttga aatccccgcc
     6601 tgggcggcca gcagatcgaa cggccgggtt tggatcggca ggtcctgctg cagcgcgcgg
     6661 atcaggtcct tgtccatctc cgtcaacggt tcggcatcgc tggcttcaac gggctgatgt
     6721 ttcaccgcct caggctttgc cgctccagcg tttccgccga ggtccagctt cacgccgatc
     6781 ttgtacatct tcagcgtcgg cagcagccgg gtcgccagcg cgccgctctg ctgatgcagg
     6841 atgcgcgcgg tgcgctgcag cccaagccgg ctgtccggcg gcaccgccag cgtgtaccaa
     6901 aggttgtacg catgatcgcg tcggtaattg tgcgagacac cgggatgcgc actgatgacc
     6961 gccgccgcgg cgtctagctg ttcggcgggg atgcgcgccg cgaccagcgt gctctgataa
     7021 ccgagggact tgctgtcgaa gattgcgctg atctggcgga tgaccgcgcg cgtgccccag
     7081 cgcagggcgg agagacgctc gatcacgtcc tgctcgctcg cgccgacgtg gtctgcgagc
     7141 gccttgtacg gccgcggcac cagcggcacc tcttcctgca ccgcctgcag cagccgttca
     7201 tccagatcgt tcaacgcgtg gtctgacatc acaactctcg tcctggcgta gcgacgatca
     7261 gatcgcggcc gccatgtggt ccatcaggtc gtcggcgtcc atgctgcgca gttcgtacaa
     7321 cacctggttc accgctgctt gccagcgacg tgccggggcg gttgccgcgg cggtcagcac
     7381 cggcaggcga ccaccgggcg cgccgacggc gacgcgtagt gtcagctctt ccgattcgat
     7441 cgtcgcgaac gcgccgatgg gcgagtggca gtcgcccttc aaaccggtca cgatcgcgcg
     7501 ctccgcgcga acgcaacggg cggtgatcgg atcgtgcagc aacgacagca ctccgcgggt
     7561 gcgttcgtcg tcgcggcagc actgcagcgc cagcgcccct tgccccgccg ccggcagcat
     7621 ttcttctgtt ggaatcgcgg ccatcgtagc ggcgtcaaac aggcaggcgc gcttcaggcc
     7681 cgccagcgcg gcgacgatcg cgtcatattc accgtcgcgg gatttacgaa gccgtgtatc
     7741 gaggtttccg cgcagcggga cgatttccag gtcgggtcgc gccgccagca gttggcagcg
     7801 acgccgcagg ctgccggtgc cgacgcgagc cgccgccggc aggtccatca cgcgatgtgc
     7861 gatgctcgac accagcacat cgcgcgggtc ttcgcgcagc ggaattgcgg ccagcaccag
     7921 gttgtcctgc gccaccagcg gcatcgtcac cggcacgtcc ttcagcgaat gcacggcgaa
     7981 gtcgatctca ccggccagca gggccaattc cagttccttc gtgaacagcc ccttgccgcc
     8041 ctcttcatgc agcgggcggt ccttgaagat gtcgccgctg gttttgtaga tgaccagctc
     8101 gacgctgagt cccgggcagc ggcgcgacaa ttgctcggcc accatggcgc tttgcgtgcg
     8161 cgccagctgg ctgccgcgcg tgcccagtcg caggacggat gtcggcgatt gcttggtcat
     8221 gatgcccgca ccttctgtcg cttgccgttc gcgcccgcgg ccgcttcttc gatattgtcg
     8281 ctcgcttcgt gcagcgcccc cgccagcgtc ttccacgtct gtccgttgtg atgccggcgc
     8341 acgctgacga cgcaggggtg catga
//
LOCUS       DATIMH010001164         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_855842, whole genome shotgun sequence.
ACCESSION   DATIMH010001164 DATIMH010000000
VERSION     DATIMH010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855842"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VLI90_20025"
     CDS             <1..607
                     /locus_tag="VLI90_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16562.1"
                     /translation="SAGPLQVPADGSNAATSKVQSLGISGIGGVLDLTNNDFILDYTG
                     TTPVTTIRRWLLTGISSGTGIISSLANSTMRLGYADNATFGALNFGGLQVDSTSILIE
                     YTVAGDTNLDGHVDVSDLGALATNYGLAANWTGGDFTYDGVVDVADLGVLASNYQTTG
                     GAAQAMASAGSAGSADSVPEPALTGVLAIAALCATTRRHRK"
     gene            610..>2557
                     /locus_tag="VLI90_20030"
     CDS             610..>2557
                     /locus_tag="VLI90_20030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16563.1"
                     /translation="MDHRLRICVLALVCLVLCTAAKAENINQFWLAAQHDWSANRGFF
                     LALEAPIPAGQPSRIEDVKLVLGVGDGSQWKFISARVDWKLDQKYQVKAVIKDGTAQL
                     SIDGQRVANEPARVAPAAVELGFNAQPSFLRGPATYQVRETKLIARGNGDAIEPQLGH
                     SALPPQLEVLSGGGVDESHQTLKIEDSVMIDAEFTFVAARSLSAIAPLVDRYGQAIAA
                     NWPAKVRDDADLKTADAEEAHRFAEWGEPTAFDAYGGLKSSPWKSGATGFYRVVTHGG
                     KWWLITPTGLPCFYTGLCDAPALDWEATPVSGREFLFSWLPPQDGQFASAWRINPWQS
                     TDEAGAHYLAWQTVNLIRKFGPDWQTQGTQSAVRRAKALGFNGFGKWSSAGCGVPDLP
                     VLNHARVPNLAGHPDVFDPKIQAAFEQALRQQIEPRLKDASLLGWSVGNEKEECIAPE
                     EITKILAMPDDVPAKQAFIQYAQSHGGTSLADTEALRRFYAERYYNFIYKTVKAIDPN
                     HLYFGMWVTPNWWVNDGDWAAVAPNCDVIGYDFYALRFDAEPMGPLIAANDKPVLCGE
                     FSAPPHYDGARGFGRYHINSADDAAAGEVYKNWLTGAAKNRNCVGVMYFQYRDQPITG
                     RGPGLGDANTAISGEDYAFGIVDVT"
BASE COUNT          486 a          809 c          835 g          427 t
ORIGIN      
        1 cagcgccgga ccgcttcagg tcccggccga cggatcgaac gcggccacca gcaaagtgca
       61 atcgctgggc atctccggaa tcgggggtgt gctcgacctg accaacaacg acttcatcct
      121 cgattacacc ggcacgaccc ccgtcaccac catccggcgg tggttgctaa ccggcatcag
      181 ctcgggcacc ggcatcatca gttccctcgc caattcgacc atgcgcctgg gctacgccga
      241 caacgcgacc ttcggcgcgc tgaacttcgg cgggctgcag gtggactcga cgagcatcct
      301 gatcgagtac accgtcgccg gcgacacgaa tctcgatggg catgttgatg tcagcgattt
      361 gggcgcgctg gcgacgaact atggattggc cgccaactgg accggcggtg acttcacata
      421 cgacggtgtg gtggatgtcg ctgatcttgg agtactcgca tcgaactatc aaacgaccgg
      481 cggcgcggca caagcgatgg catcggcggg cagcgcgggc agcgccgaca gcgttccgga
      541 gccggcgttg acgggcgttc tcgcaatcgc cgcgctgtgt gcgacgacac gccggcatcg
      601 gaaatgaaca tggatcaccg cctgcgtatc tgcgtgttgg cgctcgtctg cctcgtgctt
      661 tgcacggcgg ccaaagccga gaacatcaac cagttctggc tggccgccca gcacgactgg
      721 agcgcgaatc gcgggttttt tctcgcgctg gaagcgccga ttccagcggg ccagccgtcg
      781 cgcattgaag atgtgaagct ggtgctgggc gtgggcgatg gctcgcaatg gaagttcatc
      841 tcggcccggg tcgattggaa gctggatcaa aagtatcagg taaaagccgt catcaaggat
      901 ggcactgccc agctatcgat cgacggccaa cgcgtcgcga atgaaccggc cagagtggcg
      961 ccggcggcgg tcgaactggg attcaacgcc caacccagtt ttctgcgcgg gccggccacg
     1021 tatcaggtgc gcgagacgaa actgatcgcc cgcgggaacg gcgacgcgat cgaaccgcag
     1081 ttgggtcatt cggcgcttcc gccgcagctc gaggtgctca gcggcggcgg tgtcgatgaa
     1141 agccatcaga cgttgaagat cgaagattcg gtgatgatcg acgcggaatt cacgttcgtt
     1201 gccgcgcgca gcctcagcgc gatcgcgccg ctggtggatc gatacgggca agcgatcgcc
     1261 gccaactggc cggcgaaggt gcgtgacgat gcggatctga aaaccgccga cgccgaggag
     1321 gcgcatcgct tcgccgaatg gggcgagccg actgcgttcg acgcctatgg cggactcaaa
     1381 tcgtcgccgt ggaaatccgg cgccaccggc ttctatcgcg tcgtcacgca cggtgggaaa
     1441 tggtggctca tcacgccaac gggcctgccg tgcttctaca cggggctgtg cgatgcgccg
     1501 gcgctggatt gggaagcaac gcccgtcagc gggcgcgagt ttctcttcag ctggttgccg
     1561 ccgcaagacg gtcagttcgc gtccgcgtgg cggatcaatc cctggcaatc gaccgacgaa
     1621 gcgggcgcgc actacctcgc ctggcagacg gtcaacctga tccgcaaatt cggccccgac
     1681 tggcaaacgc aggggacgca gtcggcggtc cgccgggcga aggcgctggg cttcaacggc
     1741 tttggcaagt ggtcgtccgc cggttgcggc gtgccggacc tgccggtgct caaccacgcg
     1801 cgcgtgccga acctcgcggg tcatcccgat gtctttgatc cgaaaatcca ggctgccttc
     1861 gagcaggcgc tgcgccagca gatcgagccg cggctgaaag acgcgtcgct gctgggttgg
     1921 tccgtcggca acgaaaaaga agagtgcatc gcgccagaag agatcacgaa aatcctggcg
     1981 atgcccgacg acgttcccgc caagcaggcg ttcatccagt acgcgcagtc gcacggcggc
     2041 acgtcgctgg ctgacaccga agcgctccgc cggttctacg ccgagcgtta ctacaacttc
     2101 atctacaaaa ccgtgaaggc gatcgacccg aatcacctgt acttcggcat gtgggtcacg
     2161 cccaactggt gggtgaacga cggcgactgg gcggccgtcg cgccgaattg cgacgtcatc
     2221 ggctacgact tctacgcgct gcgattcgac gccgaaccca tgggcccgtt gatcgcggca
     2281 aacgacaagc cggtactgtg cggcgaattc tcggccccgc cgcattacga cggcgcgcgc
     2341 ggcttcggcc ggtatcacat caactcggcc gacgacgccg ccgcgggcga ggtgtataaa
     2401 aactggctga ccggcgccgc gaagaaccgc aactgcgtcg gggtgatgta tttccagtat
     2461 cgcgaccagc ccatcaccgg ccgcggtccc gggctgggcg acgcgaacac cgccatcagc
     2521 ggggaagatt acgcgttcgg aatcgtcgac gtaacgg
//
LOCUS       DATIMH010001165         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1434239, whole genome shotgun sequence.
ACCESSION   DATIMH010001165 DATIMH010000000
VERSION     DATIMH010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434239"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VLI90_20035"
     CDS             <1..556
                     /locus_tag="VLI90_20035"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HSV16564.1"
                     /translation="SNCSALGLYTGAGVPPQTVKNFVGIIKAYTTRVGGGPFPTEQAN
                     ATGDYIRERGNEYGTTTRRPRRCGWFDAMAVKYSVDLCGVTALAVTLLDVLSGLDHVQ
                     ICTGYKMNGSRLDYFRADMDTLAEVEPIYETLPGWRGNISGCRTFEELPKEAQQYVKR
                     LEQLCGAPIKMVSVGAERAATLLR"
     gene            complement(575..961)
                     /locus_tag="VLI90_20040"
     CDS             complement(575..961)
                     /locus_tag="VLI90_20040"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSV16565.1"
                     /translation="MSSGIIDSSILIDCLRGRTDAIAFLTAQSTVNRARTHLLVAAEL
                     LAGARDKQEMATIDSFLRAFDLAIPNEADGLAALDLYRQFRLSHGVDWPDCQIAATAQ
                     RIGVEIFTQNFKHFIAFPGLRVVRAY"
     gene            complement(954..1184)
                     /locus_tag="VLI90_20045"
     CDS             complement(954..1184)
                     /locus_tag="VLI90_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16566.1"
                     /translation="MTYRGKVDHGVVVLVGEKPPEGTWVDVIPATEGPTPPDSLASHP
                     AVGVWKDRTDLPEDSVEASKMLRERLMRRADE"
     gene            1210..1371
                     /locus_tag="VLI90_20050"
     CDS             1210..1371
                     /locus_tag="VLI90_20050"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HSV16567.1"
                     /translation="MKPTRLDYFRADMDTLAEVEPVYETLPGWRGNISGCRRFEELPK
                     GAQQYVKRP"
     gene            1761..2864
                     /locus_tag="VLI90_20055"
     CDS             1761..2864
                     /locus_tag="VLI90_20055"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSV16568.1"
                     /translation="MVLHVLRALFILLMAAVGWFYVNSAARPLGDLNWLSLAIALTVG
                     VFLVCIDILAPRRKLVIFSGAFLGLLIGISIAYALSFVVQLLADQFLHRLDETSATLV
                     HDRDQRLAMVNFIDMMVGVACCYLSISFILQTKDDFRFIIPYVEFSKQTKGARPILLD
                     TSVLIDGRITDIATTGLLESQVIVPRFVLDELQTVADSADRLKRARGRRGLDVLTKLR
                     SVGRAEVIIYDTSTETGEHGLEVDQRLMELAKQLNGRVLTNDYNLNKVAQLRGVDVIN
                     LNELANAMKPVVLPGEKMTVRIQKPGEEAGQGVGYLEDGTMVVVEGGRQHLDEELEFT
                     VTNTRQTVAGRMIFGRAAEGTSPPPRRPGGVRA"
     gene            3224..>3939
                     /locus_tag="VLI90_20060"
     CDS             3224..>3939
                     /locus_tag="VLI90_20060"
                     /inference="COORDINATES: protein motif:HMM:NF023853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HSV16569.1"
                     /translation="MGSNVRHEAIKTISTTRHKLDNVDFKTKHIKEQFGANVFSEEVA
                     AARLPKPIFKAFQKTIKKGMPLDPTVADAVATAMKDWAMDHGATHFTHIFQPMTGITA
                     EKHDSFLAPTIGGGAAIFEFSGKELVKGEPDASSFPSGGLRATFEARGYTAWDPTSPA
                     YIQHNPNGSTLVIPTAFLSWTGEALDKKTPLLKSMTALSQQAIRILNLFGHAGVHKVF
                     TTVGAEQEYFLIDKHFYYARP"
BASE COUNT          771 a         1204 c         1215 g          749 t
ORIGIN      
        1 cagcaactgc agcgcccttg gcctgtacac cggcgccggc gtacccccgc aaacggtgaa
       61 gaacttcgtc ggcatcatca aggcttacac cacccgcgtc ggcggcggcc cgttccccac
      121 cgaacaagcc aacgccaccg gcgattacat ccgcgaacgc ggcaatgaat acggcaccac
      181 cacgcgtcgc ccgcggcgct gcggttggtt cgacgcgatg gctgtcaaat acagcgtaga
      241 cctgtgcggc gtcaccgcgc tggccgtcac cctgctcgac gtcctctccg gcttagacca
      301 cgtccaaatc tgcaccggct ataagatgaa cggctcgcgc ctcgactact tccgcgccga
      361 catggacacg ctggcggaag tcgaaccaat ctacgaaacg ttgccgggtt ggcgcggcaa
      421 catctccggc tgccgcacgt tcgaagagct gccgaaggaa gcccagcagt acgtgaagcg
      481 gctggagcag ctgtgcggag cgccgattaa gatggtgagc gttggagcgg aacgcgcggc
      541 gacgttgctg cgataacgga ctgcgccgcg cgacttagta cgcgcgcacc acgcgcaggc
      601 cgggaaacgc gatgaaatgc ttgaagtttt gcgtgaagat ctcgacgccg atgcgctggg
      661 cggtggcagc gatctgacag tccggccaat caacgccatg agacaaacga aactggcgat
      721 acaggtccaa tgcagcaagc ccgtcggcct cgtttggtat ggccagatcg aatgcccgca
      781 ggaacgaatc gatcgtggcc atctcttgct tgtcacgggc accagccagc agttcggcag
      841 caacaagcag atgggtgcgg gctcggttaa cagtcgattg agcggtgaga aaggcaatcg
      901 cgtcggttct accgcgcagg caatcaatca ggattgagct gtcgatgatt ccgctactca
      961 tccgcccgcc gcattagacg ttcacgcagc atcttggacg cctcgaccga atcttcgggc
     1021 aggtcggtgc ggtctttcca aacacccacc gcagggtggc tggcgagcga gtcaggcggg
     1081 gtaggacctt ccgttgcagg aatgacatca acccaggttc cctccggagg cttctcaccc
     1141 accaggacaa caacgccgtg gtccactttg cctcggtaag tcatgggaaa attatacatt
     1201 cgccgcacga tgaagccaac gcgcctcgac tacttccgcg ccgacatgga cacgctggcc
     1261 gaagtcgaac ccgtctacga gacgttgccc ggctggcgcg gcaacatctc cggctgccgc
     1321 aggttcgaag agctgccgaa gggagcgcag cagtacgtga agcggccgta agtgctttgc
     1381 ggggcgccga tcaagatggt gagcgtgggg gcggaatggg ccgcgacgtt gctgcggtag
     1441 gtcactttat cctgagcgca gcacgtactc gtgcgaagtc gaaggatctt gcctggaagt
     1501 gagtgaattt tccgatcgcg cgcccggcgt cgcggtcgcc gctcaaattg ctcccgaagg
     1561 gtactcggtc agactccacc ttttttgcat caaccccggc ggcccttttg ccagcaagct
     1621 ggtcggggac tatctattat cgacacgatc cacggcgacc gattgatcgt cctggtgatc
     1681 gatgtcggcc accgcggaga tgtgtatcgc cggtgaacgc cggcggtata atccgcttca
     1741 ctcgcacggg tgggttctcc atggttctac acgtccttcg tgccctgttc attctgctga
     1801 tggccgccgt cggctggttt tacgtcaaca gcgcggcgcg gccgttgggc gatttgaact
     1861 ggctttcgct ggcgatcgcg ctgactgtgg gcgtgtttct ggtttgcatc gacatccttg
     1921 ccccgcggcg gaagctggtc attttttccg gtgcgtttct gggtttgctg atcgggattt
     1981 ccatcgccta cgccctcagc ttcgtcgtgc agctgctagc ggatcagttc ctgcatcggc
     2041 tggacgagac ctcggccacg ctggtgcacg atcgcgatca gcggctggcg atggtgaact
     2101 tcatcgacat gatggtcggc gtcgcctgct gctacctgtc gatcagcttc atcctgcaga
     2161 ccaaggacga ctttcgcttc atcatcccgt acgtcgagtt cagcaaacag actaaggggg
     2221 ctcggccgat cttgctggac acgagcgtgc tgatcgacgg gcgcatcacc gacatcgcga
     2281 cgacggggtt gctggaaagc caggtgatcg tgccgcggtt tgtgttggat gagttgcaga
     2341 cggtcgcgga tagtgctgat cgactgaaac gcgcgcgggg gcggcggggg ctggatgtgt
     2401 tgacgaagct gcggagtgtt ggccgggccg aggtcatcat ttatgacaca tcgaccgaga
     2461 cgggcgagca tgggctggag gttgaccagc ggctgatgga gctggccaag cagctgaacg
     2521 ggcgggtgct gaccaacgat tacaacctga acaaggtggc gcagctgcgc ggggtggacg
     2581 tgatcaacct gaacgagctg gccaacgcga tgaagccggt ggtgctgccg ggcgagaaga
     2641 tgaccgtgcg catccagaag ccgggcgaag aagcggggca aggcgtgggt tatttggaag
     2701 acggcacgat ggttgtggtc gaaggcggcc ggcaacattt ggatgaagag cttgagttca
     2761 cggtgaccaa cacgcgacag acggtggcgg gccggatgat cttcgggcgc gcggcggaag
     2821 gcacaagccc accgccgcgg cgaccgggcg gggtgcgggc gtaacccttc ttaccgtcta
     2881 aagctggcga tggtatcgcc ggttcttttc cagccgaccg gcgatgccat cgccggcttt
     2941 tcagggttca tttcaacagc ttgcgaaatg cgcgcttcgt gagcgcgcca ccatgtcgtc
     3001 gatggcactt gcctaattcg cgcgcggctt ttcgcgcgaa atcgcccttc tgcgccgctg
     3061 cacggctttt gcttagacca tcatcgcgaa cgggccatcg cgcgagttca ttttgcttga
     3121 cactcgaatc gccttgcaag aatatgtcgg cccgcgcgtc tgaaccaccc gcccgggcag
     3181 gtgtggtggc cgttacactt ttttaatctt tgaggagcct tacatgggtt cgaacgtacg
     3241 ccacgaggcg atcaaaacca tctcgaccac ccgccacaag ctggacaacg tcgacttcaa
     3301 gaccaagcac attaaggaac agtttggcgc caacgtcttc agcgaagagg tggccgccgc
     3361 gcggttgccc aagccgatct tcaaggcgtt tcagaagacc atcaagaagg gcatgccgct
     3421 cgatccgacc gtcgccgacg ccgttgccac ggcgatgaag gattgggcga tggatcacgg
     3481 cgcgacacac ttcacgcata ttttccagcc gatgaccggc atcaccgccg agaagcacga
     3541 cagcttcctg gcccccacca tcggcggcgg ggccgcgatc ttcgaattca gcggcaagga
     3601 actggtcaag ggtgaacccg acgccagctc gttcccctcg ggcggcctgc gcgccacctt
     3661 cgaagcccgc ggttacaccg cctgggatcc gaccagcccg gcgtatatcc agcacaaccc
     3721 caacggctct acgctggtca tccccacggc cttcctgagc tggaccggcg aagcgctcga
     3781 caagaagacc ccgctgctca agagcatgac cgcccttagc cagcaggcca ttcgcattct
     3841 caacctcttc ggccacgccg gcgtccacaa ggtcttcacc accgtcggtg ccgagcagga
     3901 atacttcctc atcgacaaac acttttacta tgcccgccc
//
LOCUS       DATIMH010001166         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5190355, whole genome shotgun sequence.
ACCESSION   DATIMH010001166 DATIMH010000000
VERSION     DATIMH010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5190355"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VLI90_20065"
     CDS             complement(<1..557)
                     /locus_tag="VLI90_20065"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HSV16570.1"
                     /translation="MSTVLMKSELPFPVRRGKVRDVYDLGDTLAGQPALLIVATDRIS
                     AFDVIMPNGIPEKGIILTALSLFWFEKFAAQFENHLIAHEVSKYPKALHPYAEQLRGR
                     SMLVKKAAVVAIECVARGYLAGSGWKEYRQNRSVCGNPLPGGLQQCQKLPQPIFTPAT
                     KEESGHDINISYAEAASRVSEETAGA"
     gene            756..2504
                     /locus_tag="VLI90_20070"
     CDS             756..2504
                     /locus_tag="VLI90_20070"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV16571.1"
                     /translation="MRRPRLLLFLIILASAGVYLIGNGSVALWDRDEPRYAQTSRQML
                     QSGDWVVPHYLDNVRTAKPVFIYWCQAAAMNIFGDNAFAARFPSAIAMVLLLIVLSFA
                     LWGHLGPERTVWTVFIFATSGLTIAAAKMCITDAVLLLWVTIAQLCLYAMWRGDRSWP
                     VVITMSIAIGLAGLTKGPVVLGFQATTLLILAILRWLDRGLASSTGVSPVSSDLRHGR
                     DARATAELPLEVSPNVLEYRTPSVKQSLDWPSILRKSLVALLIIAAITLPWIILVNQR
                     AHAFLKTAVGHDVWDRMMTPLEQHTGPPGYYLITIWITFFPWSLLLPLVIGLAIRYRA
                     HPHTRFALAAIIGPWIMLECIRTKLPHYLLPVFPPLAFLTADALVRNLHRQHDDIRRH
                     GFVIGVGVFSLLVALAASAPWIVVRAYQPLPYGAMVALSILGVVFAITVFTAFARRRI
                     AAAVSAMGVGCMLFVVVAYGWYLPRADFLRLSPRIAQVLIDHGVTQPHQVIMMRYMEP
                     SLAFHQGGTIREAGDLTLTPRWVDQLPPWIVVERGVFNQAPAEVRDQFEVVADLFGLA
                     YADKGTWMHVLVIHRK"
     gene            2665..3786
                     /gene="waaF"
                     /locus_tag="VLI90_20075"
     CDS             2665..3786
                     /gene="waaF"
                     /locus_tag="VLI90_20075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HSV16572.1"
                     /translation="MSTPQKILVVQPNWVGDAVMATPTLRAVRELYPDAHISYLMRRY
                     VKDLYGGMPWADQILTYRTGKTKAKAGKGTFDLAGRLRAGRFDLAILLPNSFKTALVC
                     KMANIPRIFGYDRDGRGFLLTDRLLPVKDKGKFVPTPIVYSYLGLARYLGSKHRDVRM
                     QLFVTDSDRRDAQDVLARCGLDRDLSRPASAGQPPLIVLNPGAQYGAAKLWKSDCFAE
                     LADRFIDDLGATVLLSAAPRERTIVESIQRRMKHAAVDLSTKGMTLGSLKEIIRRCDL
                     MVTNDTGPRHIAAAMDVPVVTVFGPTHPEWTEIYFAKERQVAVKVFCGPCQKKTWPLD
                     HRCMTRVTPAMVWDAAMQLLSPARATEPPLGRQIPLSVL"
BASE COUNT          675 a         1383 c         1187 g          761 t
ORIGIN      
        1 gcgccggccg tctcctcact cacgcgcgac gcggcttcgg cgtagctgat gttgatgtcg
       61 tgccccgact cttccttcgt cgccggggtg aagatcggct gcggcaattt ctggcactgc
      121 tgcaatccgc ccggcagcgg attgccgcac acgctgcggt tctggcgata ctccttccac
      181 ccagatccgg ccagataccc acgcgccacg cattcgatcg ccaccaccgc ggctttcttc
      241 accagcatcg accggccacg cagctgctcg gcgtacggat gcaacgcctt cggatacttc
      301 gaaacctcgt gcgcgatcag gtggttctca aactgtgccg cgaacttctc gaaccagaac
      361 aagctgagcg ctgtgagaat gatccctttt tcgggaatgc cgttgggcat gatgacgtcg
      421 aacgcgctga tgcgatcggt cgcgacgatc aacagcgccg gctgccccgc gagcgtgtcg
      481 cccaggtcat aaacatcccg caccttcccc cgccgaaccg gaaagggcag ctcactcttc
      541 atcaagacgg tggacatgcg gtcaaatcta cgtccgtgcc acgcaaagcg aaagcactgc
      601 cggaaaaccg cacacactgc cgcttgcggc tgcgcgaatc acgccatccg gtctgcgaag
      661 ccgcaagcgg cggggaggat ggaaaacccg agcatcatcc aacttcctta ctctcccttc
      721 ccccacgccc gtcgctcccg tacctttccg cgaccatgcg tcgcccgcgc ctgctcttgt
      781 tcctcatcat cctcgccagc gcgggcgtct atctcatcgg caatggctcg gtcgcgctgt
      841 gggaccgtga tgagccgcgt tacgcgcaga cgtcgcggca gatgctgcag tcgggcgact
      901 gggtcgtgcc gcattacctc gacaacgtgc gcacggccaa gccggtcttc atctattggt
      961 gccaggcggc ggcgatgaac atcttcggcg acaacgcgtt cgccgcgcgc ttcccatccg
     1021 ccatcgcgat ggtcctgctg ctaatcgtgc tgtcgttcgc gctctggggt cacctcggcc
     1081 ccgagcggac cgtctggacc gtcttcatct tcgccaccag cggcctgacg atcgccgccg
     1141 cgaagatgtg catcaccgac gccgtcctgc tgctgtgggt gacgatcgcg cagctgtgcc
     1201 tctacgcgat gtggcgcggc gaccgatcgt ggcccgttgt gatcacgatg tcgatcgcaa
     1261 tcggactcgc cggactgacc aaaggaccgg tcgtcttggg atttcaagca acgacgttgc
     1321 ttatcctcgc catacttcga tggctcgacc gagggcttgc aagtagcacg ggcgtctcgc
     1381 ccgtgtcgag cgaccttagg cacgggcgag acgcccgtgc tacggcagaa ctgccgctcg
     1441 aagtgtcccc aaatgtgctc gaatatcgta cgccctcagt taagcaatcg ctcgactggc
     1501 cttcgatctt gcgcaagtcc cttgttgcgc tgctgatcat cgccgccatc acgctcccct
     1561 ggatcatcct cgtcaaccag cgcgcccacg cgttcctgaa aaccgccgtc ggccacgacg
     1621 tctgggaccg catgatgacg ccgctcgagc agcacaccgg cccgcccggc tattacctca
     1681 ttaccatctg gatcacgttc ttcccctgga gcctgctgct gccgctggtg atcggccttg
     1741 caattcgtta ccgcgcgcac ccgcacacgc gcttcgcgct ggccgcgatc atcggcccct
     1801 ggatcatgct cgagtgcatc cgcaccaagc tgccgcacta cctgctgccg gtcttcccgc
     1861 cgctggcgtt tctcaccgcc gatgcgctgg tgcgcaacct gcaccgccaa cacgatgaca
     1921 tacgccgcca cggcttcgtc atcggcgtcg gcgtcttcag tctcctcgtc gcgctcgcgg
     1981 cgtcggcgcc gtggatcgtc gtgcgcgctt atcaaccgct gccgtacggg gcgatggtcg
     2041 cgctatcgat cctcggcgtg gtcttcgcca tcacggtctt tacagcgttc gcgcgccgcc
     2101 gcatcgccgc cgccgtctca gcgatgggcg tcggctgcat gctcttcgtc gtcgtcgcct
     2161 acggctggta tttgccgcgc gcggatttcc ttcgcctttc cccgcgcatc gcgcaggtcc
     2221 tgatcgacca cggcgtcacg cagccgcatc aggtgatcat gatgcgctac atggaaccga
     2281 gcctcgcgtt ccaccagggc ggcacgatcc gcgaagccgg cgacctgacg ctgacgccgc
     2341 ggtgggtcga tcagctgcct ccgtggatcg tggtggagcg cggcgtgttc aaccaggcgc
     2401 cggcggaggt gcgagatcag ttcgaggtcg ttgcggatct gttcggactg gcgtacgcgg
     2461 ataagggaac gtggatgcac gtgctggtga tccatcgtaa gtgacggccc ccgggcgcga
     2521 gtggcgccgt cgtttagccg ccgcggcctc gcggcgctct actacgtccg cgccgccagg
     2581 ccgcggcggt taaacgttgg cgatttcgta tttcgccacc attttttact gccggcgcat
     2641 cccgcgttat actgcacgtc gtcgatgtct accccgcaga agattttggt cgtgcagccc
     2701 aattgggtcg gtgacgccgt gatggcgacg ccgacgcttc gcgcggtccg cgagctgtac
     2761 cccgacgcgc acatcagcta cctgatgcgc cgctacgtca aagaccttta cggcggcatg
     2821 ccgtgggccg atcagatcct cacctatcgc acgggcaaga ccaaggccaa ggcaggcaag
     2881 ggcacgttcg acctcgccgg tcgcctgcgc gcggggaggt tcgacctcgc gatcctgctg
     2941 cccaattcat tcaagaccgc cctcgtctgc aagatggcga acatcccgcg catcttcggc
     3001 tacgaccgcg acggccgggg ctttctgctg accgaccgat tgctgccggt gaaggacaag
     3061 ggcaagttcg tgccgacgcc gatcgtctat tcgtacctcg gcctggcgcg atatctcggt
     3121 tcgaaacatc gcgacgtgcg gatgcaattg ttcgtgaccg acagcgaccg ccgcgacgcg
     3181 caggacgtcc tggcgcgatg tgggctggac cgcgatctgt cgcgtcccgc cagcgcaggt
     3241 caaccgccgc tgatcgtgct caatccaggc gcgcagtatg gcgccgccaa gttgtggaag
     3301 tcagattgct ttgcggagtt ggccgaccga ttcatcgacg atctgggcgc gacggtgctg
     3361 ctgagcgccg ccccgcgcga gcggacgatc gtcgagtcaa tccagcgccg catgaagcac
     3421 gccgccgtcg atttatcgac gaagggaatg acgctcgggt cgctcaaaga aatcatccgc
     3481 cgctgcgatc tgatggtgac caacgacacc ggcccgcggc acatcgccgc ggcgatggac
     3541 gtgccggtcg tcaccgtctt cggcccgacg cacccggaat ggacggagat ctatttcgcg
     3601 aaagaacgcc aggtcgcggt gaaagtgttc tgcggcccat gccagaaaaa aacctggcca
     3661 ctcgaccacc gctgcatgac ccgcgtgacc cccgcgatgg tgtgggacgc ggcgatgcag
     3721 ttgctatcac ccgcccgcgc gacagagccg ccgcttggac ggcaaattcc gttgagcgtt
     3781 ctttaagacg atcgaatctc cccccacgcc gggttcggag cgtacgcgcg acgacccggc
     3841 ttcttctgta gcgcgcgaaa agaatgcggg tcgtcggagt acctccgaac cccgcgtggt
     3901 cagcgcatca gcgcccatcc ccaagccaca atgatcaccg cctcggcaac cgcatccagc
     3961 agatagatct tcgggatcac tcgcgtgccg acgtaaaacg catcca
//
LOCUS       DATIMH010001167         1970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2296522, whole genome shotgun sequence.
ACCESSION   DATIMH010001167 DATIMH010000000
VERSION     DATIMH010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2296522"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..273)
                     /locus_tag="VLI90_20080"
     CDS             complement(1..273)
                     /locus_tag="VLI90_20080"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HSV16573.1"
                     /translation="MSSATTTTSHETIRKWIEKHKGHPAMVRTGKTGGVLRIDFDEPG
                     GNDDSRLERVGWDEFFKVFDDSDVAFLHGEGDSRFNKFVAKESAES"
     gene            578..1681
                     /locus_tag="VLI90_20085"
     CDS             578..1681
                     /locus_tag="VLI90_20085"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HSV16574.1"
                     /translation="MTTRHPSSIRSSLLRGAAAAAVALSAIAGASSAAAAPNVSVYST
                     GFNNPRGLKFGPDGMLYVAEGGTGGTQQTIGQCQQVVPPLGPYSGSTTGGRISKVDSS
                     GHRTTVTASFPSSQTTPLTGGLTSGVADIAFIGSTMYALTAGGGCSHGVANSTNGIYR
                     VSANGSKTLIADLSAYQKAHPVAQPPVRDFEPDGTWYSMVAVRGALYAVEPNHGELDK
                     ITTSGTISRVADISASYGHIVPTAVAYRGNFYVGNLNIFPIVSGSSKIIKINPAGQMQ
                     TAVWGLTIVTGVAFDAHNRMFVLENTVGAPGPAPGLGDIVRVEPSGKKTVVVDGLSVP
                     TAMTFGPDGALYVSNAGFGAPPGAGQILRITGL"
BASE COUNT          342 a          670 c          615 g          343 t
ORIGIN      
        1 ctagctctcg gccgattcct tggcgacgaa cttgttgaag cggctgtctc cctcgccgtg
       61 caggaaggcg acgtcgctgt cgtcgaacac cttgaagaac tcgtcccagc cgacccgctc
      121 caggcggctg tcgtcgttac cgccgggctc gtcgaagtcg atccgcagga cgccgccggt
      181 cttgccggtg cgcaccatcg ccgggtgccc cttgtgcttc tcgatccatt tgcggatggt
      241 ttcgtggctg gttgtggttg tcgcactcga catggcgtcc tccgtcgatt tttggcttcc
      301 caacctcacg aacgtctcag gaacgcgacg ggttcacgcg gtgaatttgc attcatagta
      361 ctagacttaa tcttaatttg acggtccccg gctgcgggcg cagcctctca ccgcaagcct
      421 gaccggtccg agtcgcgcgc tgttcgtcgc cgcgtccatt ccgacgggtt cgcaagtggc
      481 gttacttacg actgcgtccg tcaagcgcgt tgaccccgcg gcccagcatt tgcgctgccg
      541 tgcgggcgaa ctcacgcgaa cagggagggc ttagccaatg accaccagac acccatcgtc
      601 tatccgcagc agcttgcttc ggggggccgc cgcggccgcc gtcgcacttt cggcgatcgc
      661 cggcgcgagc agtgccgctg ccgcccccaa cgtttcggtc tattcgaccg gattcaacaa
      721 tccgcgcgga ttgaaattcg ggcccgacgg catgctttat gtcgccgaag gcggcaccgg
      781 cggaacgcaa cagacgatcg gacaatgtca gcaggtcgta ccgcccctcg gtccctattc
      841 cggcagcacg accggcggcc gaatttccaa ggtcgacagc agcggacatc gcacgaccgt
      901 caccgcgagc ttcccgtcga gccagaccac cccgttgacg ggcggcctga ccagcggcgt
      961 cgctgacatc gccttcatcg gcagcacgat gtacgcgctc accgccggcg gcggttgctc
     1021 acatggcgtc gccaactcga ccaacggcat ctatcgggtg agtgcgaacg gcagcaagac
     1081 gctgatcgcc gacctgtccg cctatcagaa ggcgcacccg gttgcgcagc ccccggtccg
     1141 cgacttcgag cccgacggca cctggtacag catggtcgcg gtgcgcggcg ctctgtacgc
     1201 ggtcgagccg aaccacggcg agctcgacaa gatcaccacc agcggcacta tcagccgcgt
     1261 cgccgacatc agcgccagct atggccacat cgtgccaacg gcggtcgcct atcgcggcaa
     1321 tttctacgtc ggcaacctca acatcttccc gatcgtcagc ggatcgtcga agatcatcaa
     1381 gatcaatccc gccggccaga tgcagacggc ggtgtggggc ctgaccatcg tcaccggggt
     1441 cgccttcgac gcgcacaacc gcatgttcgt gctcgagaac acggttggcg cgccgggacc
     1501 ggcgccgggg cttggcgaca tcgtccgcgt cgagccgtcg ggcaagaaga ccgtcgtcgt
     1561 cgatggcctt tcggtgccca ccgcgatgac gttcggaccg gacggcgctc tctacgtgtc
     1621 gaatgcgggc tttggcgcac cgcccggcgc cggccagatc ctgcgcatca ccggtctctg
     1681 atccgctctc cccgcggccc ggagctgccg ccgtgcagtt ccgggttgtg gcggggcatt
     1741 gaggaacggc ccgcttcgcg gctatgctga cggcggcaac gccgccagga gggaagcatg
     1801 gccaagggtc agatgaagtc caacaaggaa gcccgcaaac cgaagaaggc caaggtgaag
     1861 acgatcgccg ccaatccttc gaccaaggga tcggggggcc tcagcaagga ctgagcctga
     1921 gggtccggac ctgggtccgg ggaacacaaa agggtgcccg ggcgttcatg
//
LOCUS       DATIMH010001168         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1434854, whole genome shotgun sequence.
ACCESSION   DATIMH010001168 DATIMH010000000
VERSION     DATIMH010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434854"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VLI90_20090"
     CDS             complement(<1..277)
                     /locus_tag="VLI90_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16575.1"
                     /translation="MRLIHAALAASLFLCLLIGCNQQRPVYYQIKSQFDVSDPQFAQT
                     MGNLLGPSLISGNTIKTLVNGDETFSDMLGAIHSATTTIDFETYVYWS"
     gene            701..1864
                     /locus_tag="VLI90_20095"
     CDS             701..1864
                     /locus_tag="VLI90_20095"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HSV16576.1"
                     /translation="MAGKAKPALLRQLNDRAVFELLLDHGQASRAELTRHMGVSPTTA
                     SKTVARLLRAGFLEEVGTAAATKAGRPGRIYRLGSGSAQVLGAAIDVRRSCVLAAGLD
                     GRIHPEHVYDFPTPRSYDRLVEALTERAQHLMQTRNMPTLGMGISVPGGIDYRRQRVL
                     LSPNLHMTDGHSPSADLSGRLGIETVLAHEIRGACLAERAYGVARGMDNFVMVWAYAG
                     FGAAAVTGGRVLQGHDLMAGELGHITVERNGIECGCGNRGCLETVATDAAFARRVSDR
                     VGRTMEIEEIRQATREGRLDPTAELNETLDYLAIGIAAAINIFNPEAVLVCASSLDVQ
                     PDALDRLKQRVEARTLRPLLRNCRVLRAKGDTRQGAVASIIHHLTHALGPEMA"
BASE COUNT          364 a          601 c          733 g          361 t
ORIGIN      
        1 cggaccaata gacgtaggtt tcgaagtcga tcgttgtcgt cgcggagtga atcgcgccga
       61 gcatgtcgct gaaggtctca tcgccgttga ccagcgtctt gatcgtgttg ccggagatga
      121 gcgaggggcc gaggaggttg cccatggtct gcgcgaattg cgggtcgctg acatcgaact
      181 gcgatttgat ctggtagtag acgggccgct gctgattgca gccgatcaag agacacagaa
      241 ataatgacgc cgccagcgcc gcatggatca accgcaagat gcctcctggc gaatgcgatt
      301 gttacaatgc aaatcgcgtg cggcaatggg tgccggcgat gccgatcttg gcggatttag
      361 aacagcgcgc ggaagatggg cggtttgtaa tcctaaatcc gggagggaat cgtggccggg
      421 cgctgcatcc tccagcgcag gggtgttcgc cggagtcgaa ggatcttgcc gagaagttga
      481 tgaacgattg ctcgcgcgat cggcccggca gtggtttgcc cttcgcggag tcgccgcggc
      541 attcggcgcg aaggggcacg caactgcagc gccttcgcgt gaacgatcat ccgccaactt
      601 ccggttggag cgcctttcgt tccatcgtag cgcccttgcg cgaaactggc cgcgctgcaa
      661 acaattgcac ccggcgaatg attgcgaata atggtcgtcc atggccggta aggcgaagcc
      721 cgcgctgctg cggcagttga acgatcgcgc cgtgttcgag ctgttgctcg atcatggtca
      781 agcctcgcgc gctgaactca cccgccatat gggcgtgagc ccgacgaccg cttccaagac
      841 ggtggcgcga ttgctgcgcg ccggtttcct ggaagaagtc ggcaccgccg cggccacgaa
      901 ggcgggtcgg ccggggcgaa tctatcgcct gggcagcggg agcgcgcagg tgctgggcgc
      961 ggccatcgac gtgcgtcgat cgtgcgtctt ggcggcgggg ttggacgggc ggattcaccc
     1021 ggagcacgtt tacgattttc ccacgccgcg gagttacgac cgcctggtgg aagcgctgac
     1081 cgagcgggcg cagcacctga tgcagacgcg gaacatgccg acgctgggga tgggcatcag
     1141 cgtgccgggc gggatcgact atcgccggca gcgcgtgctg ctgtcgccga acctgcacat
     1201 gaccgatggg cattcgccca gcgccgactt gagcggccgg ctgggcattg agacggtgct
     1261 ggcccacgag atccgcgggg cgtgcctggc cgagcgcgcc tatggcgtgg cgcgcgggat
     1321 ggacaacttc gtgatggtct gggcgtacgc gggtttcggc gccgcggccg tgacgggcgg
     1381 gcgcgtgctg cagggccacg acctgatggc cggcgaattg ggtcacatca ccgtcgaacg
     1441 caacggcatc gaatgcggct gcggcaatcg cggttgcttg gaaacggtgg cgaccgacgc
     1501 cgcgtttgcc cggcgggtgt cggaccgcgt cgggcggacg atggagatcg aagaaatccg
     1561 ccaggccacc cgcgaaggcc ggctcgatcc gaccgcggag ctgaacgaga cgctggatta
     1621 tctggccatc ggcatcgcgg cggcgatcaa catcttcaac cccgaagcgg tgctggtgtg
     1681 cgcgtcgtcg ctggacgtgc agccggatgc gctggaccgc ctgaagcagc gcgtggaggc
     1741 aagaacgctg cgaccgctgc tgcgcaattg ccgggtgctg cgcgccaagg gcgacacgcg
     1801 acagggggcg gtggcgagca tcattcatca cctgacgcac gcgctgggcc cggagatggc
     1861 gtgaggtcgt cgatctggct cgggagtcag tgaaatttca ctcgcgcgag tcccgccgct
     1921 tgcggcttag cgtcttttgt acccaaggaa cagatgctaa gcgcaagcgg cggtgagcgc
     1981 taattgcaaa gcaatgcttc accgactcct cggagggatc cttcgactcc ggcgagtacg
     2041 cctgcgctgg aggatgaca
//
LOCUS       DATIMH010001169         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4026149, whole genome shotgun sequence.
ACCESSION   DATIMH010001169 DATIMH010000000
VERSION     DATIMH010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4026149"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VLI90_20100"
     CDS             <1..183
                     /locus_tag="VLI90_20100"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSV16577.1"
                     /translation="ALAWLLHNPVVTAPIIGPRTMDQLTDALKVPDLKLEPHTLESLD
                     KIWPGPGGEAPKAYAW"
     gene            complement(292..762)
                     /locus_tag="VLI90_20105"
     CDS             complement(292..762)
                     /locus_tag="VLI90_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16578.1"
                     /translation="MNEKEMGKALLHGEHPINISELTRGVLRRDRRRMWILGTLCVIA
                     WMLVVMMPWATILPMLAKVVEHFAHADPFASPPATTRPQSAEVVELLQIVKLGTMATF
                     IGSIASMFMAAVCTVSLIILSRRATLRQVNARLAEISDQLKALMGTEERKAKPR"
     gene            complement(759..1331)
                     /locus_tag="VLI90_20110"
     CDS             complement(759..1331)
                     /locus_tag="VLI90_20110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV16579.1"
                     /translation="MNDAAERLYERVLVVRCQTGEPVALAELIARYAPRLRFYLAKMA
                     GEGVADDLLQDVWVNVFANIARLNDAGAFGGWLYRIARDKAYRELRRRRTPMLPFDEA
                     SIEAAIGEAEDETDWTAEDVERVRAALDRLPPEQREVLVLQFVEDMNYQQIAALIACP
                     VGTVRSRIHYAKRALRSALDESAVRKDLRT"
     gene            1520..2248
                     /locus_tag="VLI90_20115"
     CDS             1520..2248
                     /locus_tag="VLI90_20115"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSV16580.1"
                     /translation="MPISRHIIVVIVLCIAGASRLSLAQALEPTSRPTDKAVDPSFVD
                     VPDDPNLPRVLLIGDSISMGYTIPVREMLKGKANVHRPPANCGQTQRGLDQLDKWLGN
                     GKWDVIHFNFGLHDLKYLDASGKYVDPSKGKQVAPPEVYEKNLRELVARLKKTGAKLI
                     FATTTPVPAGTLGRVEGDEVRYNKVAARVMADTGVAIDDLHAVAMKDQQTIQRPRNVH
                     FTPEGYKMLAASAAASIERALQGP"
     gene            complement(2302..>2509)
                     /locus_tag="VLI90_20120"
     CDS             complement(2302..>2509)
                     /locus_tag="VLI90_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16581.1"
                     /translation="DAGVHTFSVTLNVAGTQTISVFDTLVSSIVGSDTLTVSTPTSGG
                     GGGGGTGGGGTGGGGGGGGGKATA"
BASE COUNT          462 a          817 c          772 g          458 t
ORIGIN      
        1 gccctcgcct ggctgctgca caaccccgtc gtcaccgcgc cgatcatcgg cccgcgcacg
       61 atggatcagc tgaccgatgc gctgaaggtg ccggacctga agctggagcc acacaccttg
      121 gaatcgctgg acaaaatctg gcccggaccc ggtggtgaag cgcccaaggc gtacgcgtgg
      181 tgagctctac gtattcacac gcgccttcat agccgcaggc gacagccagc gggggcgctc
      241 gagtaccagc ccggttgatt gtcgcaatcc cccgctggct atcgcccgcg gctatcgtgg
      301 cttcgccttt cgctcttccg ttcccatcag agccttgagc tgatccgaaa tctccgccaa
      361 ccgcgcgttc acctgccgca gcgttgcgcg gcgcgacagg atgatcagcg acaccgtgca
      421 caccgccgcc atgaacatgc tggcgatgct gccgatgaac gtcgccatcg tgcctaactt
      481 gacgatctga agcagttcca ccacctccgc cgattggggc cgtgtggtcg ccggcggtga
      541 cgcaaatgga tcggcgtggg cgaagtgctc gaccaccttc gccagcatcg gcaggatcgt
      601 cgcccacggc atcatcacca ccagcatcca ggcgatgacg cagagcgtgc cgaggatcca
      661 catgcgccgc cgatcgcggc ggagcacgcc gcgggtcaat tcgctgatat tgatcggatg
      721 ctcgccgtgc agcagcgctt tgcccatttc cttctcgttc atgtgcgcag gtcctttctt
      781 actgccgatt catccagcgc cgaacgcagc gcgcgctttg cgtagtggat gcgtgagcgc
      841 accgtgccca ccgggcaggc gattaacgcc gcgatctgtt ggtagttcat gtcctcgaca
      901 aactggagca ccaacacttc gcgctgctcg ggcggcaatc gatccagcgc cgcccgcacg
      961 cgctccacat cttcggcggt ccaatccgtc tcatcctccg cctcaccgat cgccgcttcg
     1021 atcgacgctt cgtcgaaagg aagcattggc gttcgccgcc gccgcaactc gcgataagcc
     1081 ttgtcgcgcg cgatgcggta aagccagcct ccaaacgccc ccgcgtcgtt tagccgggcg
     1141 atgttggcga aaacattgac ccacacgtct tgaagcaggt cgtcggcgac gccttcaccg
     1201 gccattttcg cgaggtagaa ccgcaggcgc ggcgcatagc gcgcgatcag ctccgccagc
     1261 gccaccggct cgccggtctg gcagcggacg accagcaccc gctcatacag ccgctcggcg
     1321 gcgtcgttca tcgccggtta ggttgcgctg ggatgagaaa tggttcaaat gaaatgcgat
     1381 atttcacgcg atgcgaggac acgtgatagc cgggggcggg agccccggtc tttttccttc
     1441 aatccttcaa attcgaaagg accggggctt ccgcccccgg ctatcatggt tcacctgcca
     1501 tgctgatatg attcagcgca tgccgatctc ccgacacatc attgtcgtca tcgttctgtg
     1561 catcgccggg gcttctcgtt tatcgctggc gcaagcgctg gagcccacgt cgcggccgac
     1621 ggacaaggcg gtagatccgt cgttcgtcga cgttcccgat gatccgaatc tgccgcgcgt
     1681 gctgctgatc ggcgattcga tatcgatggg ttacacaatc cctgtgcgcg agatgctcaa
     1741 aggcaaagcc aatgtgcatc gcccgccggc gaattgcgga cagacgcaac gcgggctcga
     1801 tcagctggac aagtggctcg gcaacggcaa gtgggacgtg atccacttca acttcggcct
     1861 gcatgatctg aagtatcttg atgcgagtgg caagtacgtc gacccgagca aaggcaagca
     1921 ggtcgcgccg ccggaggtgt atgaaaagaa tctgcgcgag ctggtggcgc ggctgaagaa
     1981 gacgggcgcg aagttgattt tcgcaacgac gacgccggtg cccgccggaa cgctggggcg
     2041 cgtggaaggg gacgaggtgc gctacaacaa agtcgccgcg cgggtgatgg ctgacaccgg
     2101 cgttgcgatc gacgatctgc acgcggtggc gatgaaggac cagcagacga tccagcggcc
     2161 gcggaacgtt cacttcacgc cggagggata caaaatgctc gcggcctccg cggccgcgag
     2221 catcgagcgg gctttgcagg gcccgtgagt atgccgagca gatctcaatt gttgcggtcc
     2281 ttccggcaca tctggactgc cttaagcggt ggcttttccc ccgccgcctc caccgccacc
     2341 gccggtccca ccaccgccgg tgccaccgcc accgccgcca cccgaggtgg gagtggatac
     2401 ggttagggtg tcggagccga cgatcgagct gacgagggtg tcaaagacgc tgatcgtctg
     2461 cgtgccagcg acgttgaggg tgacgctgaa cgtgtgaacc ccggcgtcc
//
LOCUS       DATIMH010001170         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_856953, whole genome shotgun sequence.
ACCESSION   DATIMH010001170 DATIMH010000000
VERSION     DATIMH010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856953"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VLI90_20125"
     CDS             <1..454
                     /locus_tag="VLI90_20125"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16582.1"
                     /translation="IDLATLTGGVITALGKTMAGVMSNSDAIVAELDRASKTAGEKFW
                     RLPLGDEQREQMKSDFADILNSAGREGHPLQGGAFLSYFVPEDRSVPWAHLDIAGVAD
                     TEKDQPFYSKGATGWGVRTLVEWVTNRASGGGGLNPEATQSTPPPAGG"
     gene            459..1709
                     /locus_tag="VLI90_20130"
     CDS             459..1709
                     /locus_tag="VLI90_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16583.1"
                     /translation="MGLLAKFFGPPSREKFSRLVARQLRERGESRPIQFDPERFCLRL
                     GDNAHTMFLQNAYVEYCRTPRARRTDVLQRAASTVELGRRPEPALDEVRSNLLPRVRE
                     RVYFSSFDLNTRIERLIRPPETNADKPPLENFIIQPFAEQLTLEIVIDTPTAIQSLSR
                     KLVEKWGVSFDELLPIAKDNLWRMSNQDFPQVAPGVYLSPWRDTHDASRLFLHDLIWQ
                     LKVKGDHVAAVPNRDILLVTGSEDVDGLKAMAALAEEALKQPRPMTGTFFRLSGTQWS
                     PFLPSPDSPAYWPVKGLAMRVIANDYAEQTSLLTTLHEKSKQDVFVAKQALMRRDDGS
                     FWTWAVWVDGVDDALLPKADFISFGAVENGQKRQDGWAPWSSVVSVLGHRMEATDVYP
                     PRFRVRTYPTVDERAALQLMKEPT"
     gene            1706..2485
                     /locus_tag="VLI90_20135"
     CDS             1706..2485
                     /locus_tag="VLI90_20135"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSV16584.1"
                     /translation="MSEAQTADRWDAQLYDARHAFVWRYGAGLLDLLDPKPGERVLDV
                     GCGTGHLTKQIADRGATVVGVDASSAMIDEARRNFPELTFQIADATRMRLDEPFDAVF
                     SNATLHWVKPPEAAVARMFEALKPSGRLVIEMGGKGNIQRVLDAAAAAGREMGLELHS
                     LLHTNYFPSVAEYSTLLERQGFEVSLATLFDRPTQLQGDAGLREWIRMFRGEVLHAVP
                     AATLDAFFTAMERIARPQLLRDNSWYADYRRLRVVASRNAG"
     gene            2494..3237
                     /locus_tag="VLI90_20140"
     CDS             2494..3237
                     /locus_tag="VLI90_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16585.1"
                     /translation="MPETFLILLSAGVMFAAAVPRPLDVTLRWLRLAGMIALSLTALS
                     LFLWLRRGEIGSANQGALYLAVAVLILGQLAMTQTARANAQRVFALLATVAGVALAVR
                     LLSSASWKIALSCAGVAVMCGVALMDMLLGHAYLTASKMTMRPFHRLNDTLGITAVIR
                     LIVATAGVLWLNSRSPVPMLWGVYGLYMITRWLVGLAVPLVFVYMARDCIARRSTQSA
                     TGILYVAGVLIFIGEILALNLARETGLPF"
     gene            3237..3569
                     /locus_tag="VLI90_20145"
     CDS             3237..3569
                     /locus_tag="VLI90_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16586.1"
                     /translation="MTNATSPKPLTACPHCGCQDLFVRKDFPQRLGLAIVVLAGVSFL
                     VLAARPTTFRWGVWVLIAAVAVDAVLYFFVPRVTTCYRCRADFRDVPINPSHGAFELA
                     VAEKYRGR"
BASE COUNT          592 a         1037 c         1283 g          687 t
ORIGIN      
        1 catcgacctg gcgacgctga ctggcggggt gatcacggca ctcggcaaga cgatggccgg
       61 cgtgatgagc aacagcgatg cgatcgtggc ggagctggat cgcgcttcga agacggccgg
      121 cgaaaagttc tggcgcctgc cgttgggcga tgagcagcgg gagcagatga agtcggactt
      181 cgcggatatc ctcaacagcg ccggccgcga aggtcacccg ctgcagggcg gggctttcct
      241 cagttacttc gtgcccgagg accgttcggt gccgtgggcg cacctcgaca tcgccggcgt
      301 tgccgacacg gagaaggatc agccgttcta tagcaagggc gccaccggtt ggggcgtgcg
      361 tacgctggtt gagtgggtga ccaatcgcgc cagtggcggc ggtggcttga atccagaagc
      421 cacgcaatcg acaccaccgc cggcgggagg gtagcggttt gggcctgctc gcgaaattct
      481 tcgggccgcc ctcgcgcgaa aagttttcgc gcctcgtggc gcgccagctg cgcgagcggg
      541 gcgagtcgcg accgatccag ttcgatcccg agcgcttctg cctgcggctg ggcgacaacg
      601 cgcacacgat gttcctgcaa aacgcgtatg tcgaatactg ccggacgccg cgcgcgcggc
      661 ggaccgacgt gctgcagcgg gcggcatcca cggtcgagct gggccggcgg ccggagcccg
      721 cgctggacga ggtgcggtca aacctgctgc cgcgcgtgcg cgagcgggta tatttcagct
      781 cgttcgactt gaacacgcgg atcgaacgcc tcatccgtcc acccgagaca aatgccgaca
      841 aaccaccgct cgagaacttc atcatacagc ctttcgccga gcaactgacg ctggagatcg
      901 tgatcgacac accgacggcg atccagtcgc tgagccgaaa gctggtggag aagtggggcg
      961 tgagcttcga tgagctgttg ccgatcgcga aggacaacct ttggcggatg agcaaccagg
     1021 attttcccca ggtcgctccg ggcgtttacc tctcgccctg gcgcgacacg cacgatgcat
     1081 ctcggttgtt cctgcacgat ctgatctggc agctgaaggt gaagggggat cacgtggcgg
     1141 cggtgccgaa tcgtgacatc ctgcttgtca cgggcagcga ggacgtcgat ggactgaagg
     1201 cgatggcggc gttggcagaa gaagcgctga agcaaccgcg gccgatgacg ggcacgttct
     1261 tccggctcag cgggacgcag tggtcgccgt ttctgccgtc cccggatagt cccgcgtatt
     1321 ggccggtcaa aggattggcg atgcgcgtga tcgccaacga ttacgccgag caaacatcct
     1381 tgctcaccac gctgcacgag aagtcaaagc aggatgtctt cgtcgccaag caagcgctga
     1441 tgcggcgcga cgacggcagc ttctggacat gggccgtgtg ggttgacggt gtcgatgacg
     1501 cgctgctgcc aaaggcggat ttcatttcct tcggcgcggt ggagaacgga cagaagcggc
     1561 aggatgggtg ggcgccgtgg tcgagcgtcg tcagcgtgct ggggcatcgg atggaggcga
     1621 cggacgtgta tccgccgcgg ttccgggtgc gaacttatcc gacggtcgac gagcgcgcgg
     1681 ccctgcagtt gatgaaggag ccgacgtgag cgaagcgcag acagccgatc gatgggatgc
     1741 gcagctgtac gacgcgcggc acgcgtttgt gtggcgatac ggcgctggac tgctggattt
     1801 gctcgatccc aagccgggcg agcgcgtgct ggacgtcggt tgtggcaccg ggcatctcac
     1861 gaagcagatc gccgatcgcg gggcgacggt ggtgggtgtc gatgcatcat cggcgatgat
     1921 cgatgaagcg cggcggaact ttccggagct gacattccag attgcggatg caacgcggat
     1981 gaggctcgat gagccgttcg acgcggtgtt ttccaatgcg acgttgcatt gggtgaagcc
     2041 gccggaggcg gcggtggcgc ggatgttcga ggcgctgaag ccaagcggac ggctggtgat
     2101 cgagatgggc ggcaagggaa acatccagcg cgtgctggat gccgcggcgg cggcggggcg
     2161 ggaaatgggt ttggagttgc actcgctgct gcatacgaac tatttcccga gcgtcgcgga
     2221 atactcgacg ctgctggagc ggcagggatt tgaggtgtcg ctggcgacgt tgtttgatcg
     2281 accgacgcag ttgcagggcg acgcggggtt acgcgaatgg atccgcatgt tccgcggcga
     2341 ggtgctgcat gccgtgcccg cggcgacgct ggacgcgttc ttcaccgcga tggagcggat
     2401 cgcccggccg cagttgcttc gcgacaatag ttggtacgca gactatcgcc ggctgcgcgt
     2461 cgttgcatcc aggaatgccg gttgaacccg ccaatgccgg aaacattcct gatcctcttg
     2521 tcggcaggcg tgatgttcgc ggcggcggtg ccgcggccgc tggatgtgac gctgcgctgg
     2581 ttgcggttgg cggggatgat cgcgctgtcg ctgacggcgc tgtcgctgtt cctgtggctg
     2641 cggcgtgggg aaataggatc ggccaatcaa ggtgcgcttt atcttgcggt tgcggtgctg
     2701 atcctgggcc aactggcgat gacgcagacg gcacgcgcga atgcccaacg ggttttcgcg
     2761 ctgctggcaa cggtcgccgg cgtggcactg gcggttcgac tcctcagttc agcgtcgtgg
     2821 aagatcgcgc tctcctgcgc tggcgtcgcg gtcatgtgcg gcgtagcgct gatggacatg
     2881 ctgctcggtc acgcgtatct gacggcgtcg aagatgacga tgcggccgtt ccatcgcctg
     2941 aacgacacgc tgggcattac cgccgtcatc cggctgatcg tggcgacggc gggggttttg
     3001 tggctcaact cgcgctcgcc cgtgccgatg ctgtggggcg tgtacgggct atacatgatc
     3061 acgcgctggc tcgtcgggct ggcggtgccg ttggtgttcg tgtacatggc gcgcgattgc
     3121 atcgcccggc gatccacgca gtccgccacc ggcatccttt acgtcgccgg tgtgttgatc
     3181 ttcatcggtg aaattctcgc gctgaacctg gcgcgcgaga cgggactgcc gttctgatga
     3241 ccaacgcgac gtcaccgaaa ccgctgaccg cctgcccgca ctgtgggtgc caagacctgt
     3301 tcgtccgcaa ggattttcca cagcgactcg gcctggcgat cgtcgttctg gccggcgtct
     3361 cgttcctggt gttggcggcg cgaccgacga cgttccgctg gggcgtgtgg gtgctgatcg
     3421 ccgcggtggc cgtcgatgcg gtgctctact tcttcgtccc gcgggtgaca acctgctatc
     3481 gctgtcgcgc cgacttccgc gatgtgccga tcaatccgtc gcatggcgcg tttgagcttg
     3541 cggtggcgga gaagtatcgt gggcgatgag cgaaattctt cgaacgcatt ggaggcttc
//
LOCUS       DATIMH010001171         4174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_857507, whole genome shotgun sequence.
ACCESSION   DATIMH010001171 DATIMH010000000
VERSION     DATIMH010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857507"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VLI90_20150"
     CDS             complement(<1..817)
                     /locus_tag="VLI90_20150"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HSV16587.1"
                     /translation="MRIALAQINPTVGDIAGNVRKILDFTGRAKTAGAALVVFPELSV
                     TGYPPKDLLLKPQFVDDNLRAVRMIAAHAPRDIDIIVGYAEQNTDPVGRPLHNAVAVL
                     RDGVIASRHFKTLLPTYDVFDESRYFEPGPVNATENLFKVNGTMIGQSICEDLWNDEK
                     LITRRLYHQNPIADLNAAGAEILVNSSASPFVVNKHEFRLELFGAQARRFKKPIVYVN
                     QVGGNDELVFDGNSVVFDADGKVIAQARDFEEDLMVVELSRGTGASPVQHELDP"
     gene            complement(894..2234)
                     /locus_tag="VLI90_20155"
     CDS             complement(894..2234)
                     /locus_tag="VLI90_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16588.1"
                     /translation="MIKFPCKCGNVFNLTEDMAGGLIQCTRCGLLNDIPSLEDAPFIN
                     EDGTFELEEATDVTDGIALPELHEAFSPSTVDAQGREKDLRNTIATYDQIDTRALDAA
                     AERVAPKYDPATGELIRPLEVKDEAPTPVVPLGEDAGGAMPVAAIPVAPIVNPKTVGY
                     ALRDTQKKLTPFTLAIELLMPANVIVLFFLFLFYVFTGIIGTFIAVGSLYFQVSLQVL
                     NFPLWLILAHYGCVIEDTGPDNREELPRPLRHFALSEDIWNPFFNVALAAFISYAPAF
                     IAAMPRWHLGSAQSPLVMLLGLFGCAVLPAVLLTTVTGVSLLNLRPDRVASVMRICGA
                     DYIMAVGIYMLALVPTVFYLVLPKILPANSTNPIITHMHEPYVTLPLLAVSVYLGHYF
                     CWLLGLMYRDHHDEFPWLMQRHVKDPMARAFDVVGRTPREPRRAQKQPRRGQLR"
     gene            2465..3667
                     /locus_tag="VLI90_20160"
     CDS             2465..3667
                     /locus_tag="VLI90_20160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSV16589.1"
                     /translation="MRIRRIISTVIVLVLLGGLARLLWKAGAFAPEREPVRPIPTAKV
                     ELKDIEEDVLATGEVSPVDQTEIRSEVSGQVTQLQVKPGERVEKDKQLLQLDRRELES
                     QAKELQFQIAADELRAKQAKMELDRDNELISQGFIPQKEYDDANIALMLAQNDLEVQR
                     AKLETLRQQIIKTDIRSPHAGIVLKLDAREGEVIIGASSVSSGTVLMRIADLSRLKVD
                     TRLNEVDVVKVNVGDKVQLTFDAVPNRTIAGTVTYISPSADTGESSNQNGGGGGQRGS
                     SGGGGSVRGFETIVALDEIDPRIRPGITAHVKISMSKVSKAVAAPLTAVFMDGKRSVA
                     YVKKGEQFERREIEPGISDSTVVEIKKGLAAGDEIATEKPADDKLAPTTAPATGPTTS
                     ATTTTAVN"
     gene            3857..>4174
                     /locus_tag="VLI90_20165"
     CDS             3857..>4174
                     /locus_tag="VLI90_20165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSV16590.1"
                     /translation="MALVEFQNVEKTYRMGGTSVRALDGVSFGIDPGEFVSIIGPSGS
                     GKSTLMHLLGFLDQPTGGTISFDGQDVSKISPRQRATIRANRIGFVFQAFNLLPRLSV
                     LQNA"
BASE COUNT          837 a         1272 c         1340 g          725 t
ORIGIN      
        1 gcggatccag ttcatgctgc acgggcgagg cgcccgtgcc acgggacagc tcgacaacca
       61 tcagatcttc ttcgaagtct ctcgcctgcg cgatcacctt tccgtcggcg tcgaagacga
      121 cgctgttgcc gtcgaacacc agctcgtcgt tcccgccgac ctggttgacg taaacgatcg
      181 gcttcttgaa gcgtcgcgcc tgcgcgccga agagctcgag gcggaattcg tgcttgttga
      241 cgacgaacgg ggacgcgctg gagttgacca ggatctccgc gccggccgca ttcaaatcgg
      301 cgatcgggtt ctggtgatac agccggcggg tgatcagctt ctcgtcgttc cacagatctt
      361 cgcagatgct ctgcccgatc atcgtgccgt tgaccttgaa cagattctcc gtcgcgttca
      421 ccggccccgg ttcgaaatat cggctctcgt cgaacacatc gtacgtcggc agcagcgtct
      481 tgaaatgccg gctggcgatg acgccgtcgc gcagcaccgc gacggcgttg tgcagcgggc
      541 gaccgacggg atcggtattt tgctcggcgt agccgacgat gatgtcgata tcgcgcggcg
      601 cgtgagccgc gatcatccgc accgcgcgca ggttgtcgtc cacaaactgc ggcttgagca
      661 gcaggtcctt cgggggataa ccggtgacag ataattcggg aaagaccacc agcgccgcgc
      721 ccgccgtctt cgcgcggccg gtaaagtcga ggatcttgcg aacattcccc gcgatgtccc
      781 cgacggttgg attgatctga gcaagcgcga tccgcatagt cgcgcattct aggacggcgg
      841 cgggatcgaa acaaaagccc acggatggct atacgtgggc tttgctttcg acttcaccgc
      901 agttgtccgc gccgcggttg tttctgggcc cgtcgcggtt cgcgcggcgt gcgcccgacc
      961 acgtcaaacg cccgcgccat cggatccttc acgtgccgtt gcatcagcca ggggaactca
     1021 tcgtgatggt cgcgatacat caacccgagt agccagcaga agtagtgccc gagatacacc
     1081 gacaccgcca gcagcggcag cgtcacgtac ggctcgtgca tgtgcgtgat gatcgggttc
     1141 gtcgaattgg ccggcaggat cttcggcaac accagataaa acaccgtcgg caccagcgcc
     1201 agcatgtaaa tgccgaccgc catgatgtaa tccgcgccgc agatgcgcat gaccgaagcc
     1261 acgcggtccg gccgcaggtt cagcagcgaa acgccggtca cggtcgtcag cagcaccgcc
     1321 ggcaggacgg cgcaaccgaa tagcccaagc agcatgacca gcggcgactg cgcgctgccc
     1381 aggtgccagc gcggcatggc cgcgatgaac gcgggggcgt atgagatgaa ggccgcgagc
     1441 gcgacgttga agaatggatt ccaaatgtct tcgctcagcg cgaagtgacg cagcgggcgc
     1501 ggcagctctt cgcgattgtc aggtcccgtg tcttcaatca cgcagccgta gtgcgccagg
     1561 atcagccaga gcggaaaatt cagcacctgc aatgacacct ggaaatacag gctgccgacc
     1621 gcgatgaacg tgccgatgat tcccgtgaag acgtagaaga ggaagaggaa gaacagcacg
     1681 atcacgttgg cgggcatgag cagttcgatt gccagcgtga acggcgtcag cttcttctgc
     1741 gtgtcgcgca gcgcgtatcc aacggtcttc ggattgacga tcggcgccac cggaatcgcc
     1801 gcgaccggca tcgcgccgcc ggcgtcttcc cccaacggca ccaccggcgt cggcgcttcg
     1861 tccttcacct ccagcgggcg gatcaattca ccggtggccg ggtcgtactt gggcgcgacc
     1921 cgctccgccg cggcgtccag cgcgcgggtg tcgatctgat cgtatgtcgc aatcgtattg
     1981 cgcaggtcct tctcgcggcc ctgtgcatcc accgtgctcg gcgagaacgc ctcgtgcagc
     2041 tccggtagcg cgatgccatc ggtcacgtcc gtcgcttctt ccaattcgaa cgtgccgtct
     2101 tcgttgatga acggcgcatc ttccagcgac gggatatcgt tcagcaatcc gcagcgagtg
     2161 cattggatca gaccaccggc catgtcctcg gtgaggttga agacgttgcc gcacttgcag
     2221 gggaacttaa tcatgtcgcg ggtcacgagc gtcgggcaat gattatacgc cgcagaagac
     2281 ggataggatg cagcctgctt tcaaaccgtt catgcaaagc tcactcgttg tctccgtctc
     2341 atgatgtgga gcctccccgc gtttgattta ccgccgtcgc tgcacgacat tggcgaggcg
     2401 aagaaaaagc ccacggctct gccgtgggtc ccgagtgtgg caagagaatc cgagggcact
     2461 gacgttgcgc atccgccgaa tcatttccac ggtcatcgtg ctggtcctgc tcggcgggct
     2521 tgcccggctt ttgtggaaag cgggcgcgtt tgcgccggag cgcgagccgg tgcgcccgat
     2581 ccccaccgca aaggttgagc tgaaggacat cgaggaagac gtgctggcga cgggcgaggt
     2641 gtcgccggtc gatcagacgg agattcggtc cgaagtcagc gggcaggtca cgcagctgca
     2701 ggtgaagccg ggcgagcgcg tggagaagga caagcagctg ttacagctgg atcggcggga
     2761 gttggagagc caggcgaagg agctgcagtt ccagattgcc gccgacgaat tgcgggccaa
     2821 gcaggcgaag atggagctgg accgcgacaa cgaattgatc agccaggggt tcatcccgca
     2881 gaaggaatat gacgacgcga acatcgcgct gatgctggcg cagaacgact tggaagtgca
     2941 gcgggcgaag ctcgagacgc tgcggcagca gatcatcaag accgacatcc gctcgccgca
     3001 cgcggggatc gtgctgaagc tggatgcgcg cgagggcgaa gtcatcatcg gcgccagcag
     3061 tgtctccagc ggcacggtgc tgatgcgcat cgcggacctg agccgcctga aggtggacac
     3121 gcgcttgaac gaagtggacg tcgtgaaagt aaacgtcggc gacaaggtgc agctgacgtt
     3181 cgatgcggtg ccgaaccgca cgatcgcggg gacggtcacg tacatctccc cctcggccga
     3241 caccggcgaa agcagcaacc aaaacggcgg cggcggcggc caacgcggca gcagcggcgg
     3301 cgggggcagc gtgcgcgggt tcgagacgat cgtcgcgctc gacgagatcg acccgcgtat
     3361 ccgcccgggc atcacggcgc acgtgaagat ttccatgtcg aaggtgagca aggccgtcgc
     3421 ggccccgctg acggcagtgt tcatggacgg caagcgaagc gtcgcgtacg tgaagaaggg
     3481 cgagcagttc gagcgacgcg agatcgagcc ggggatcagc gacagcacgg tggtcgagat
     3541 caagaaggga ctggcggccg gcgatgagat cgcgacggag aagcccgcgg atgacaagct
     3601 ggcgccgacg acggcgccgg cgaccgggcc aacaacgagc gcaacgacaa ctactgcggt
     3661 taactaatga cgaaacccga aactcgaacc ctcgaatcaa tggcgaatgt cgaatgaccg
     3721 aatagccgaa tgacgaaacg cttacgcgga ggctccggcc agactaaccg cttcagcgat
     3781 cagtgcattc attcctcatt cgtcattggg acttgattcg gggattcgga cttcgtcatt
     3841 tggattcccg accagcatgg ccctcgtcga attccaaaac gtcgaaaaga cctaccgcat
     3901 gggcggcacc tccgtgcgcg ccctcgacgg cgtcagcttc ggcatcgatc ccggcgaatt
     3961 cgtctccatc atcggcccat ccggctcagg caagagcacg ctgatgcacc tgctcggctt
     4021 tctcgatcag ccgacgggcg ggacgatctc ttttgatggc caggacgtct cgaaaatatc
     4081 cccgcggcag cgcgcgacga ttcgcgccaa tcgcatcggc tttgtcttcc aggcgtttaa
     4141 cctgctgccg cggctgagcg tgttgcagaa tgcg
//
LOCUS       DATIMH010001172         4337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_275568, whole genome shotgun sequence.
ACCESSION   DATIMH010001172 DATIMH010000000
VERSION     DATIMH010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275568"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /gene="ettA"
                     /locus_tag="VLI90_20170"
     CDS             <1..1157
                     /gene="ettA"
                     /locus_tag="VLI90_20170"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HSV16591.1"
                     /translation="ALCRLLLQKPDVLILDEPTNHLDAETVGWLEQHLKRYEGTVIAV
                     THDRYFLDNVAGWILELDRGQGIPWKGNYSSWLEQKKARLQIEEKQESARQKALAREL
                     EWVRKSPKARQAKSKARLQAYEQMVAQDQREKEKDLEIFIPPGPRLGERVITAVNLTK
                     AYGDNVLMSNVSFTLPPNAIVGIIGPNGAGKTTLFRMVTGGEKPDAGEIKLGESVKLA
                     YVEQSRDDLAPDDTVWQAISDGQEFIKLGPVNVNSRSYVTRFAFVGSDQQRKVGTLSG
                     GERNRVHLARMLKQGANVLLLDEPTNDLDINAIRALEEALENFAGCAVVISHDRWFLD
                     RLATHILAFEGDSQVIFHDGNYTEYEADRKKRLGIAADQPHRIKYRKLTK"
     gene            complement(1405..2367)
                     /locus_tag="VLI90_20175"
     CDS             complement(1405..2367)
                     /locus_tag="VLI90_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16592.1"
                     /translation="MPKAPPIEQQLLELSGLRADATAPHAREKLAHAINGKNNLLAAR
                     AATVVTDTKQADYLPLLATAFDRFFASASDKGCAAKTAIANALYTLGHVDAQTFLRGV
                     RHVQMEPTFGGHADAAAELRGICGLGLVRCGYRDALLELTSLLMDKEPQTRQMAVRAV
                     VYAGHNEGAMLLRMKVLAGDESGEVIAEAFDGLMKLTPAKSLDFVARFLDDRNPATAE
                     SAAVAIGGSRTPVAYERLRQQWEHQIRPEPRRPLLLAIAMTRQPQAVEFLLERVREDS
                     AKPASDAVAALGMYKHDDAVRTKLAAAVKERDDLLINEAFAKAF"
     gene            complement(2345..3403)
                     /locus_tag="VLI90_20180"
     CDS             complement(2345..3403)
                     /locus_tag="VLI90_20180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HSV16593.1"
                     /translation="MAFSLLQPLFDIPIAVIDVETTGASADFGHSVIELGIVRVERGQ
                     RVAEYQQLLDPQRRIGPGITALTGITQAMVDGQPRFVDQIDRITQLLSGSIVMGHNVR
                     FDLSFLNKEYRRCGRQMPEVLGASTPVLDTVRIARRRFGRGGNGLGNLARRLGIDPVV
                     AHRALADAQTTHLVFEQLIAPVGGWHIPLCNAMAEQGGPMGLLPVNPRESILPLELEE
                     ALEQRKPVLMVYLDGRQHQTQRVINPLTIRRSGGELLLIAHCHLRADRRTFKVERIVE
                     LKRIDGPIPAELLQCDEPHVATVAPASSSSQDAPIDSVAATPASPSHEREEATQASPL
                     QEDASDGMIAADAQGSAD"
     gene            complement(3552..4028)
                     /locus_tag="VLI90_20185"
     CDS             complement(3552..4028)
                     /locus_tag="VLI90_20185"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16594.1"
                     /translation="MPPQLIFDISSVNLDAVIADQAAIRAVNPQRGAMEHLNGINYVD
                     SVAGVILGYKDVRADEFWVEGHIPGRPLLPGVIMIEAAAQLASYFVKSVRKWEGFIGF
                     GGVDECKFRMQVTPGCRMYVLCKELNARHRRITSAVQGLVNGQIAFETKIIGACLG"
BASE COUNT          790 a         1318 c         1436 g          793 t
ORIGIN      
        1 tcgcactatg ccggttgctg ctgcagaagc cggacgtgct gatcctggac gagccgacga
       61 atcatctcga cgcggaaacg gttggctggc tcgagcagca tttgaagcgg tacgagggca
      121 ccgtcatcgc ggtgacgcac gatcggtatt ttctggacaa cgtcgcgggg tggatcctcg
      181 agctcgaccg cgggcagggt attccgtgga aggggaacta ttcatcgtgg ctcgagcaga
      241 agaaagcgcg gctacagatc gaagagaagc aggaatcggc gcggcagaaa gcgcttgcgc
      301 gcgaattgga gtgggttcgc aagagcccca aggcccggca ggcgaaatcg aaagcgcgcc
      361 tgcaggcgta tgagcaaatg gtcgcgcagg accagcgcga gaaagaaaag gacctggaga
      421 tcttcatccc gcccggcccg cggttgggcg agcgcgtcat caccgccgtc aacctcacca
      481 aggcgtatgg cgacaacgtg ctgatgtcca acgtcagctt cacgctgccg cccaacgcga
      541 tcgtcggcat catcggcccc aacggcgccg gcaaaaccac gctcttccgc atggtcaccg
      601 gcggagaaaa gccggatgcg ggagaaatca agctcggcga aagcgtgaag ctggcgtacg
      661 tcgaacagag ccgcgacgac ctggcaccgg acgacaccgt ctggcaggcg atcagcgacg
      721 ggcaggaatt catcaagctc ggcccggtca acgtcaacag ccgatcgtac gtcacgcgtt
      781 tcgcgttcgt cggctccgat cagcaacgca aagtcggcac gctctccggc ggtgaacgca
      841 accgcgttca cttagcgcgc atgctcaagc aaggtgcaaa cgtcctgctg ctggacgagc
      901 cgacgaatga tctggacatc aacgcgatcc gcgcgctgga agaagcgctg gagaatttcg
      961 ccggctgcgc ggtcgtcatc agccacgacc ggtggttcct cgaccgcctg gcgacgcaca
     1021 tcctcgcgtt cgaaggcgac agccaggtga tcttccacga tgggaactac acggaatacg
     1081 aagccgatcg caagaagcgg ctgggcatcg cggcggatca gccgcatcgg attaagtacc
     1141 ggaagctgac gaagtgagcg tttatgtcgg tcatcctgag cgcagcggcg cagccgcgca
     1201 gtcgaaggat ttagccgggg cgttggtgaa cgttcagaat cgagaccggt acagcagtcg
     1261 cttgcgtctt cggggaaacg cggccttccg cgatgacgca agcgactgcg atgctctccc
     1321 gcggaattca tccttcacat acttctcagc aagatccttc gactccggcg aatacgcctg
     1381 cgctcaggat gacaagcgcg cgaactagaa cgccttcgcg aacgcttcgt taatcagaag
     1441 atcgtcgcgt tccttcaccg ccgcggcgag ctttgttctc accgcgtcgt cgtgcttgta
     1501 catccccagc gccgcgacgg cgtcgctcgc gggcttggcg ctgtcttcgc gcactcgctc
     1561 cagcaaaaac tccaccgcct gcggttggcg cgtcattgcg atcgccagca gcagcggccg
     1621 gcgcggctcg gggcggatct ggtgttccca ttgctggcgg agacgttcgt atgccactgg
     1681 cgtgcgcgag ccgccgatcg cgacggcggc ggattcggcg gtcgcggggt tgcggtcgtc
     1741 gaggaatcgc gcgacgaagt cgagcgactt cgccggcgtc agcttcatca ggccatcaaa
     1801 cgcctcggcg atcacctcgc cgctctcgtc gcccgccagc accttcatcc gcagcagcat
     1861 cgcgccttcg ttatgtccgg cgtagacgac cgcgcgcacc gccatctgcc gggtttgcgg
     1921 ctccttatcc atcagcagcg acgtcaactc cagcagcgca tcgcgatagc cgcagcgcac
     1981 caacccgagc ccgcagatgc cgcggagttc cgcggcggcg tcggcgtggc cgccgaaggt
     2041 tggctccatc tgcacgtgcc ggacgccgcg gaggaaggtt tgcgcatcga cgtggccgag
     2101 cgtgtagagg gcgttagcga tggcggtctt ggcagcacac cccttgtcgg aagctgacgc
     2161 gaagaaacga tcgaacgcgg tggcgagcag cgggagatag tcggcctgct tggtgtcggt
     2221 gacgacggtc gccgcccggg cggccaggag gttgttcttg ccgttgatgg cgtgggcgag
     2281 tttttcgcgc gcgtgcggtg cggtggcgtc tgcgcgcaag ccggaaagct caagcagttg
     2341 ctgctcaatc ggcggagcct tgggcatcgg cggcgatcat accgtcgctg gcatcttcct
     2401 gtagcggcga tgcctgcgtc gcttcttctc tctcgtgcga aggcgacgca ggcgtcgcgg
     2461 ctacagaatc gattggcgca tcctgcgacg aggatgatgc aggtgccacc gttgcgacat
     2521 gcggttcatc gcactgcaac agctccgccg ggatcgggcc gtcgatgcgc ttcagctcca
     2581 cgatccgctc gaccttgaac gtccgccgat cggctcgcag gtggcagtgg gcgatcagca
     2641 gcaattcacc gcctgagcgg cggatcgtca gcgggttgat cacgcgctgc gtttggtgtt
     2701 ggcggccatc caggtacacc atcagcaccg gcttgcgctg ctcgagggct tcttccagtt
     2761 ccagcggcag gatgctttcg cgcgggttga ccggcaacag gcccatcggg ccgccctgct
     2821 cggccatcgc gttgcacagc gggatatgcc agccaccgac gggggcgatt agttgttcga
     2881 agacgaggtg cgtcgtctgc gcatcggcca gcgcgcggtg cgcgacgacg gggtcgattc
     2941 ccaagcgcct ggcgagattg ccaaggccgt taccgccgcg gccgaagcgg cgtctggcga
     3001 ttctgaccgt gtccagcacc ggtgtgcttg ctccgagcac ctccggcatc tgccggccac
     3061 agcggcggta ttccttgttc aggaacgaca ggtcgaagcg cacgttgtgg cccatgacga
     3121 tcgagccgct gagcaactgt gtgatgcggt cgatctgatc gacaaaccgc ggctggccgt
     3181 ccaccatcgc ctgcgtgatg ccggtgagcg cggtgatgcc ggggccaatc cggcgttgag
     3241 gatcgagcag ctgttgatat tccgcgacgc gctggccgcg ttcgacgcgg acgatgccga
     3301 gctcgatgac gctgtggccg aagtcggcgc tggcgccggt cgtttcgacg tcgatcaccg
     3361 cgatggggat gtcgaacaga ggttgaagga gggagaaggc catggtggtc cgtcaccgac
     3421 gcgccgcttg cggcttagcg tgttttacgc tttttttgaa ataaacagac gctaagccgc
     3481 aagcggctga tgttctgtcc ctgaatagat tgtacgtgcg tggacggggg aaaggtcgaa
     3541 cgactcgtgg atcaacccag gcaggcgccg atgatcttgg tctcgaacgc gatctgcccg
     3601 ttcaccaggc cctgtacggc gctggtgatc cggcgatgcc gggcgttcag ttccttgcac
     3661 agcacgtaca tcctgcagcc gggcgtgacc tgcatgcgga acttgcattc gtccacccca
     3721 ccgaacccga tgaacccttc ccactttcgc acggatttga cgaaatacga cgccagttgg
     3781 gcggcggctt cgatcatgat gacgcccggc agcagcgggc ggccgggaat gtggccttcg
     3841 acccagaatt cgtcggcgcg gacgtctttg taacccagga tgacgccggc gacggaatcg
     3901 acgtagttga tgccgttgag atgctccatc gcgccgcgct ggggattgac ggcccggatg
     3961 gccgcctgat cggcgatgac cgcgtcgagg ttgacggagg aaatatcgaa aatcagttgt
     4021 ggcggcatcg cggggttggg ttggaaatag gaaaaggtaa aagagaactg gaagcgggtg
     4081 aaggcctgga gccgcggccc ggatcctgtg ttccctttta ccttttgaga taccttctga
     4141 acgaggtcta aactggggtg gaggcgtctg tctggaccct cacccgagat gctaaccgct
     4201 gaagcggtta gcccgggtga gggctccaca ccgcggttga tgttgggtta ttctgcggcg
     4261 tcgttggcgt cgtcaccgcc gccggtggcg ccggcgctgc cttcgccttc gcggccttcc
     4321 aggatcttct tgcggac
//
LOCUS       DATIMH010001173         4492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3454314, whole genome shotgun sequence.
ACCESSION   DATIMH010001173 DATIMH010000000
VERSION     DATIMH010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454314"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1670
                     /locus_tag="VLI90_20190"
     CDS             72..1670
                     /locus_tag="VLI90_20190"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSV16595.1"
                     /translation="MPKITVDGRVIDAKQGQMVLQACNDADVPIPQYCYHPGMSIVAS
                     CRICLVEVEGIPKLVPACQTPVRDGMVVYAKSSKAIANQKQVMEYLLINHPLDCPVCD
                     QAGECLLQDYSYQYGRSQSRFEEDKAKKPKKDVGLHVLLYNDRCIMCTRCVRFTREVA
                     GTSELYVDGRGYREEIDIFPGKPLNNKLSGNVVDICPVGALLDKDFLFKQRVWLLKQI
                     PSISPVDSGGENIYLEYNEGQVWRIKPRYNPDVNTWWISDDTRYSYKVVGDEKRLRVA
                     RKTQYGSQIDTSYSNALEAAKAGLVDLVKTNGQGCVYAMMSPMMSIEEAWLFGRAIRE
                     LDPQATLVLGPVPTTGQNEIFKHSITGKKTFEIQAEKVPNAAGIRRVLALLGGPQLAY
                     DELVKPKSTNPAVTKLKGGWIVGGYLSEWIGSVTFPRGFKVVQDILPNKLVDSADVVL
                     PAACWAEKDGSWENYAGKMQAFSAAVPPPEGTRREGDVYLNLLSRREMYSAETIRHEM
                     GEPFAAAKVETGKAEEAAFAFAEL"
     gene            1670..2281
                     /locus_tag="VLI90_20195"
     CDS             1670..2281
                     /locus_tag="VLI90_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16596.1"
                     /translation="MKSGRWSILARGVGLAMLILSGVDCQADRGVPSPPAAAPTTAPS
                     SAPTAKDVQTFEDRRAGVRFNYPSGWAPEKAATAVFDVAAPASATKGYASISLDVPSV
                     PPHFPGMITPKIICDHYVKDLRENQIHDAKVEEAVDIKAAGAPARRVKCSGHEKGKVM
                     IEVAVVIVHSERIYIFSCDADPEGYAAARAALDQAVATLEWIK"
     gene            2289..2870
                     /locus_tag="VLI90_20200"
     CDS             2289..2870
                     /locus_tag="VLI90_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16597.1"
                     /translation="MQNAELKIIFIVFILHSAFIVLPSARADTVAPSTTQSATTEFVS
                     PEFRFRLRHPAQWTRPEHPVNDQVFSVRTPDVAPGDHRFGVVGLKIDNGPEGQSDAAT
                     LLELSDSIAAYVFKGGAQHVTVKPDKIGVANILARRVRFVTEQPSGKIATMYVIAVRK
                     RTEYVFNIAAPVELFDTMLPGVDELLKSFELIE"
     gene            2972..4423
                     /locus_tag="VLI90_20205"
     CDS             2972..4423
                     /locus_tag="VLI90_20205"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HSV16598.1"
                     /translation="MDWISAHIPPWVFIAIIAHLAVLGSVAYLILLERKVASWVQDRI
                     GPNRVGLGFGIIPKLKDMHMFGLGQPLADGLKFILKEDYRPKNVDKILFTLAPGVMIT
                     VVIISIATLPWGGWHQTSRTVHVYGAANPTLRAQEQAANEIPGNAEFVGEPLLINGGA
                     DAIVTYKYPFQIANLNIGVLFILAALSLAVYGVVIGGWASNNKYSFLGGLRATANMIS
                     YEIPLGLAVLCVVATFGTLDLAVITEKQAHYWLGFIPAWNCFSQPLAFLMFLVCIHAE
                     ANRAPFDTAEAEQELVGGYHTEYSAMRFALFFLAEYAGMITTSAVCVALFFGGWHLPW
                     IEWLIPALRGDAAGGHPEVTTSLIACIVRSLIFFGKTMGIIFIFMWVRWSLPRFRFDQ
                     IMMLAWRALIPMSLLLLLATVIAGYLGGYSPDGFVTGRLAIALLIANIATLAITMIGS
                     TLVPPAPETNRRMHVAESRYARTPLPGGAVAST"
BASE COUNT          878 a         1396 c         1413 g          805 t
ORIGIN      
        1 aaatttgatg cgctcgttgc ccgcgccgag ctagtgaaat tccgcacgaa ccggtatacc
       61 attccgcccc tatgcccaag atcaccgtgg acggccgggt catcgacgcc aagcaagggc
      121 agatggtcct ccaggcctgc aacgacgccg atgttccgat cccgcagtac tgctaccacc
      181 ccggcatgtc gatcgtcgcg tcgtgccgga tttgcctggt tgaagtcgag gggattccca
      241 agctggtccc cgcctgccag acgccggtgc gcgatggcat ggtcgtttac gccaagtcat
      301 ccaaagcgat cgccaaccag aagcaggtga tggaatacct gctgatcaac cacccgctgg
      361 attgcccggt gtgcgaccag gccggcgagt gtttgctgca ggattactcc taccagtatg
      421 gccgcagcca gagccgcttc gaggaagata aagccaagaa accgaagaag gacgtcggcc
      481 tgcacgtgct gctgtacaac gaccgctgca tcatgtgcac gcgctgcgtg cgcttcacgc
      541 gcgaggtggc cggcacgagc gagttgtacg tcgatggccg cgggtaccgc gaagagatcg
      601 acatctttcc cggcaaaccg ctgaacaaca agctgtccgg caacgtcgtg gacatctgcc
      661 cggtcggcgc actgctcgac aaagatttcc tcttcaagca gcgcgtctgg ctgctgaagc
      721 aaatccccag catcagcccc gtggactccg gcggcgaaaa catctacctc gaatacaacg
      781 aaggccaagt ctggcggatc aagccgcggt acaacccgga cgtgaacacc tggtggatca
      841 gcgacgacac gcgctattca tacaaggtgg tcggcgacga gaagcgcctg cgcgtcgcgc
      901 gcaagacgca gtatggttcg caaatcgaca ccagctattc gaacgcgctc gaagcggcca
      961 aggcgggact cgttgatctg gtgaagacca acggccaagg ctgcgtctac gcgatgatgt
     1021 cgccgatgat gtcgatcgaa gaagcctggc tgttcggtcg cgccatccgc gagctggacc
     1081 cgcaggcgac gttggtcctc ggccccgtgc cgacgacggg tcaaaacgaa atcttcaagc
     1141 attcgatcac cggcaagaaa acttttgaga tccaagccga gaaggtgccc aatgccgcgg
     1201 ggattcgccg cgtgctcgcg ctgctcggcg ggccgcagct ggcgtacgac gaactggtca
     1261 agcctaagag caccaatccc gccgtgacga agttgaaggg cggctggatc gttggtggtt
     1321 acctcagcga atggatcggc agcgtgacgt tcccgcgcgg gttcaaggtt gtgcaggaca
     1381 tcctgccgaa caagctggtc gatagcgccg acgtcgttct ccccgcggcg tgctgggcgg
     1441 agaaggacgg ctcgtgggaa aattacgctg ggaaaatgca ggcgttcagc gctgccgtcc
     1501 cgccgccgga aggcacgcgg cgcgaaggcg acgtgtatct gaacctgctg agccggcgcg
     1561 agatgtatag tgcggaaacc atccgccacg agatgggcga accgttcgcc gcggcgaagg
     1621 tcgaaacggg caaagccgaa gaagccgcgt tcgcatttgc ggagctctga tgaaaagcgg
     1681 gcgttggtcc atcctcgcgc gcggcgtcgg attagcgatg ctgatcctgt cgggcgtcga
     1741 ttgccaggcg gatcgcggcg tgccgtcacc gccagccgcg gcgccgacga ccgcgccgtc
     1801 gtcggcgcca accgcgaagg acgtgcagac gttcgaagac cgccgcgcgg gcgttcgctt
     1861 caactacccc agcggatggg cccccgaaaa agcggcgacg gcggtgttcg acgtggcggc
     1921 ccccgcgagc gcgaccaaag gttacgcgtc gatcagcctg gacgtgccga gcgtcccgcc
     1981 gcatttcccc ggcatgatta cgccgaaaat aatctgcgac cattacgtaa aggacctgcg
     2041 cgagaatcag attcatgatg cgaaggttga agaggcggtg gacatcaaag ccgccggtgc
     2101 gcccgcccgg cgcgtgaagt gcagcggtca cgaaaagggt aaggtgatga ttgaggtcgc
     2161 ggtggtgatc gtacacagcg agcggatcta catcttcagt tgcgacgccg accccgaggg
     2221 atatgccgca gcgcgagcag ccttggacca ggcggtggcg acgctggagt ggattaagta
     2281 aatgcagaat gcagaatgca gaattgaaga tcatcttcat cgtattcatt ctgcattctg
     2341 cattcatcgt tctgccttcg gcgcgagccg acaccgtggc gccgtcgacg acgcagtccg
     2401 cgacgacgga attcgtcagc cccgagttcc gctttcgcct gcgtcacccc gctcagtgga
     2461 cgcggcccga gcacccggtg aacgaccagg tgttctcggt gcggacgccc gacgtggcgc
     2521 ctggcgatca tcgcttcggc gtggtcggcc tgaagatcga caacggcccc gaaggccaga
     2581 gcgacgccgc cacgttgctg gagttgtccg acagcatcgc cgcttacgtg ttcaaaggcg
     2641 gcgcgcagca cgtgacggtg aagccggaca agatcggcgt cgccaacatc ctcgcccggc
     2701 gggtgcggtt cgtgaccgag cagccgtcgg gcaagatcgc gacgatgtac gttatcgcgg
     2761 tgcgcaagcg aacggagtac gtcttcaaca tcgccgcccc ggtcgagctg ttcgacacaa
     2821 tgctgccggg cgtggacgag ctgttgaaga gttttgagtt gatcgagtga taagtagcat
     2881 gggctaccag cccatggcta gcggccatag ccgctcttca gtcacgcgta tcggttttca
     2941 ggagcatggg ctggtagccc atgccattaa tatggattgg atttctgcac acatcccccc
     3001 ttgggttttc atcgcgatca tcgcgcacct cgcggtgctc ggctcggtcg cgtacctcat
     3061 cctgctcgaa cgaaaagtcg ccagctgggt gcaggatcgc atcgggccca accgtgtggg
     3121 cctgggcttc ggcatcatcc cgaagctgaa ggacatgcac atgttcggcc tcggtcagcc
     3181 gctggccgac ggactgaagt tcatcctgaa ggaagactat cgcccgaaga acgtggacaa
     3241 gattctgttc acgctcgcgc cgggcgtgat gatcaccgtg gtcatcatca gcatcgccac
     3301 gctgccgtgg ggcgggtggc atcagacctc gcgcaccgtg cacgtgtacg gcgcggccaa
     3361 cccgacgctt cgcgcccaag agcaggcggc caatgagatc cccggcaacg ccgagttcgt
     3421 cggtgagccg ctgctgatca acggcggcgc cgacgcgatc gtcacctata aatatccgtt
     3481 ccagatcgcc aacctgaaca tcggtgtgct gttcatcctc gcggcgctct cgctggcggt
     3541 ctacggggtc gtcatcggcg gctgggcgag caacaacaaa tactcgttcc tcggcggact
     3601 ccgcgcgacc gccaacatga tcagctacga aatcccgctc ggactcgcgg tgctctgcgt
     3661 cgtcgcgacg ttcggcacgc tggacctggc cgtcattacc gagaagcaag cgcactactg
     3721 gctcggcttc atccccgcgt ggaattgctt ctcgcagccg ctggcgttcc tgatgttcct
     3781 cgtctgcatt catgccgaag cgaaccgtgc cccgttcgac acggctgaag ccgagcagga
     3841 gctggtcggc ggatatcaca ccgagtacag cgcgatgcgc ttcgcgctgt tcttcctggc
     3901 cgaatatgcc ggcatgatca cgaccagcgc ggtctgcgtc gcgctgttct tcggcggctg
     3961 gcacctgccg tggatcgaat ggctcatccc cgcgctgcgc ggcgacgcag cgggtggcca
     4021 tccggaagtg acgacgagtt tgatcgcctg catcgttcga tcgctgatct tcttcggcaa
     4081 gacgatgggc atcatcttca tcttcatgtg ggtccgctgg tcgctgccgc gcttccgctt
     4141 tgaccagatc atgatgctcg cgtggcgcgc gctgatccca atgtccctgc tgctgctgct
     4201 ggcgacggtc atcgccggtt acctcggcgg ctattcgccc gacggcttcg tcaccgggcg
     4261 cctggccatc gcgctgctga tcgcgaacat cgcaacgctg gcgatcacga tgatcggcag
     4321 caccctggtg ccgcccgcgc cggaaacgaa ccgccggatg cacgtcgcgg aaagccgcta
     4381 cgcgcgcacg ccgctcccgg gtggcgcggt ggcttcaacg tagattcgat cgggaattga
     4441 accacgaagg gcacgaagag cacgaagtga agacaaagcg atacgacaag at
//
LOCUS       DATIMH010001174         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2877865, whole genome shotgun sequence.
ACCESSION   DATIMH010001174 DATIMH010000000
VERSION     DATIMH010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2877865"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VLI90_20210"
     CDS             complement(<1..424)
                     /locus_tag="VLI90_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16599.1"
                     /translation="MVAYLGGLDEADRLGVADAASGASGASGGAGNGQRVLFDSASPR
                     FIEIDTARLRVQNVRAFGGAWLALQLIDKLGLRSFVDEAMPRGREEIPWSVMALVLVI
                     GRLLDPSSELRLAEHLYERTALADLLGVPASKVNDDRLY"
     gene            623..856
                     /locus_tag="VLI90_20215"
     CDS             623..856
                     /locus_tag="VLI90_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16600.1"
                     /translation="MANKKISSRNCGSWAKSPTHAAVEGAFPMVKADIDGQSRPTPSD
                     IGCDQISEAPITNRPLKAEDVGPSWLAPSARND"
     gene            992..1339
                     /locus_tag="VLI90_20220"
     CDS             992..1339
                     /locus_tag="VLI90_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16601.1"
                     /translation="MLLLALLVLVTGAPAQAQEPHDRAAWMLTARWGVMTHYLADWIA
                     RRERPGQKMTVEEWNNFVDHFNVEALADKSRRPAPVTTSSRSARTPASTIRPTPRTTA
                     LPASPRAIARGAI"
     gene            1384..1662
                     /locus_tag="VLI90_20225"
     CDS             1384..1662
                     /locus_tag="VLI90_20225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16602.1"
                     /translation="MVYLPSGAPGGDKATKAALQYQNGPHRNAEFQRNWEAIMREWSL
                     RWGDHVAGWWFDGCYWPNAMYRGDEPNFHTFAAAVRAGNPNSAIGLPQ"
     gene            1703..1933
                     /locus_tag="VLI90_20230"
     CDS             1703..1933
                     /locus_tag="VLI90_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16603.1"
                     /translation="MDAFVGGQWKLIASGTTIGHRKLDRFPKVMASKVRFTITKARAC
                     PVIRSIGVHMDSVSPPESFEPGNALSEPKPRR"
     gene            complement(1974..>3680)
                     /locus_tag="VLI90_20235"
     CDS             complement(1974..>3680)
                     /locus_tag="VLI90_20235"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSV16604.1"
                     /translation="VSLDEPRHKVLVLTGHSPANSPAIESLRSRCDVVEALTIDQAIE
                     LLRRDHFTAVFSDSADFLPLERALVSQQANLILNTIGEGVCIVDGEGRCSWMNKKMGA
                     WPARVHEKIRRTCQEAFDLFSHQVSPQNADTPASFSRSKRYTLNIEDQQYMEMIASPV
                     INPAGAVVQVVAVVWDATGTRRLQQKIDAIDKAGRELVRLESDAIVKMNVGQRLKLLE
                     DKIISFTRELMHFDHFAIRLLDRKSNKLEMVISAGLPPEAVNIELSADGEGNGISGFV
                     AATGRSYICPDVERDPRYVMGLDQAKSSLTVPLRLHDKVIGIFNIESRQRAAFNEDDR
                     QFAEIFGRYVAIALNILDLMVVERVSTSHKVADEVNSEVAGPLNDITLDATTLIDEYI
                     GHDDLRQKLQQILENVTLIRKSLRQAAEGTNTKILGAAEVKGEQDTIIANARILVADD
                     EPNIRTTISDVLRKYHAAVTMVTNGSEAIAQLEQGDFDLVISDIKMPDKSGYDVFAAA
                     RKHSQTVPVILMTGFGYDPNHSIVRASQEGLQAVMFKPFKVDQLLTEVRKALQPQPAV
                     RT"
BASE COUNT          693 a         1163 c         1133 g          691 t
ORIGIN      
        1 gatacagacg atcgtcgttc accttcgacg ccggaacccc cagcaggtcc gccaacgccg
       61 tgcgctcgta cagatgctcg gccaggcgca gctcgctcga tggatcgagc agccgtccga
      121 tcaccagcac caatgccatc acgctccagg gaatctcttc gcgaccccga ggcatcgctt
      181 cgtccacaaa agatcgcaga ccgagcttgt cgatcagctg cagcgccagc cacgcgccgc
      241 cgaacgcgcg cacgttctgg acgcgcaaac gcgcggtgtc gatctcgata aaccgcggcg
      301 aagcggagtc gaacagcacg cgctggccat tgccggcccc acccgatgcc cccgatgccc
      361 ccgatgcggc atcggcgaca cccagccgat ccgcttcgtc caatccaccc agatatgcta
      421 ccacgcgctg ccgaggccca cgcgcggtgc gaaccgattc aatcagtttc cagtagacgt
      481 atgttcgccc accgcaggat tgccgacatc gccgcagata cattgcccca aagaatatcc
      541 agggatggaa cggaatgttt taggcactac atcggctttc agcgccttga ccccacgtga
      601 cattcaacca gagccgattc agatggcaaa caaaaaaatc tcaagccgta actgcggaag
      661 ttgggctaag agtcccacgc acgccgccgt ggagggcgcg ttcccaatgg tgaaggccga
      721 catcgacggc caatcgcgcc cgacaccgag cgacatcggg tgcgatcaga tctccgaagc
      781 gccgatcacc aatcgcccgc tgaaggcgga ggatgtgggc ccatcatggc tggcgccatc
      841 agcgcgcaac gattgagcgg tggaatgcat gagaaacagc aaatcgcacg aatcatttaa
      901 cagccgcagg cgccagccag cgggggctct cgataatcag ccgggctggt actcgaggac
      961 ccccgccggc tgtcgcctgc ggctatcaac gatgctcttg ctcgcgctgc tcgtgctcgt
     1021 gaccggcgct cccgcccaag cgcaagaacc ccacgatcgc gccgcgtgga tgctcacggc
     1081 gcgctggggt gtgatgacgc actacctcgc cgactggatc gcccgccgcg agcgtcctgg
     1141 gcaaaagatg accgtcgagg aatggaacaa cttcgtcgat cacttcaacg ttgaagctct
     1201 ggccgataaa tcccgtcgac cagcgccggt tactacctca tcacgatcgg ccagaactcc
     1261 ggcttctacg attcgcccaa cgccacgtac gaccgcatta ccggcctcac cccgagccat
     1321 tgctcgcggc gcgatctgat cgccgacctg tacgaaccgc tgcaccgccg gggaatcaag
     1381 ctgatggtct acctgccgtc gggcgcgccc ggtggtgaca aggcgaccaa ggcagcgttg
     1441 caatatcaga acggcccgca tcgcaacgcg gaatttcagc gcaactggga agcgatcatg
     1501 cgcgaatggt cgctgcgctg gggtgatcac gtcgccggtt ggtggttcga tggctgctat
     1561 tggccaaacg cgatgtaccg tggcgatgag ccgaacttcc acaccttcgc cgccgccgtg
     1621 cgcgcgggga atccgaacag cgccatcggt cttccgcagt gaagaggcca tcgcgctggg
     1681 ccagcgcatt ccggaataca cggtggacgc gttcgttggc gggcaatgga agttgatcgc
     1741 cagtggcacc acgatcgggc accggaagct ggaccgattc ccgaaggtga tggcgtcgaa
     1801 ggtgcgcttc acgatcacca aagcgcgcgc gtgcccggtc atccgatcga tcggcgttca
     1861 catggacagc gtctcgccgc cggaaagttt tgagccgggg aacgcgctgt cggaaccgaa
     1921 gccgcgcagg tgacgcattc cgactcgctg aagcgagtcg gccggtcgaa ggattaagtt
     1981 cggacggcgg gttggggttg cagggctttg cgcacttccg tgagcagctg atccaccttg
     2041 aacggcttga acatcaccgc ctgcaggcct tcctggctgg cgcggacgat gctgtggttg
     2101 gggtcgtaac cgaagcctgt catgaggatc accgggacgg tctgcgaatg ttttcgcgcg
     2161 gcggcgaaga cgtcgtagcc gctcttgtcc ggcatcttga tgtcgctgat caccaggtcg
     2221 aagtcgccct gctccagctg cgcgatcgcc tcggagccgt tggtgaccat cgtgacggcg
     2281 gcgtgatatt tgcgcaggac atcgctgatc gtcgtgcgga tgttcggctc gtcgtcggcc
     2341 accaggatgc gggcgttggc gatgatggtg tcctgctcgc ccttcacctc cgcggcgccc
     2401 aggatcttcg tgttcgttcc ctcggcggct tgccgtagcg atttgcggat gagcgtgacg
     2461 ttttccagga tctgctggag cttctgccgc aggtcgtcgt ggccgatgta ttcgtcgatc
     2521 agcgttgtcg catcgagcgt gatatcattg agcggcccgg cgacctcgga gttcacttcg
     2581 tcggccacct tgtggctggt gctcacgcgc tcgaccacca tcaggtcgag aatgttgagc
     2641 gcgatggcaa cgtagcggcc gaagatctcg gcgaactggc ggtcgtcttc gttgaacgca
     2701 gcccgctggc gcgactcgat gttgaagatg ccgatcactt tgtcatgcag ccgcagcggc
     2761 accgtcaggc tgctcttggc ttggtccagc cccatcacgt agcgcgggtc gcgctcgacg
     2821 tcggggcaga tataactgcg acccgtggcg gcgacgaaac cgctgatgcc gttgccttca
     2881 ccgtcggcgc tcagctcaat gttgaccgct tccggcggca ggccggcgct gatcaccatc
     2941 tccagcttgt tgctcttgcg atcgagcagg cgaatggcga agtgatcgaa gtgcatcagc
     3001 tcacgcgtga agctgatgat cttgtcttcc agcagcttca ggcgctgacc aacgttcatc
     3061 ttcacgatgg cgtcggactc caggcgcacc agctcgcgcc cggctttgtc aatcgcgtcg
     3121 atcttctgct gcaaacgccg agtgcccgtg gcatcccaca cgacggccac gacctgaacc
     3181 accgcgccgg cgggattgat gaccggcgat gcgatcatct ccatgtactg ctggtcttcg
     3241 atgttcaggg tgtaacgttt ggatcggctg aaggaggcgg gtgtgtcggc gttctgcggg
     3301 gagacctggt ggctgaacag gtcaaaagct tcctggcagg tgcggcggat tttttcgtgc
     3361 acccgcgccg gccaggctcc catctttttg ttcatccaac tgcaccgccc ttccccgtca
     3421 acgatgcaga cgccttcacc gatcgtgttg aggatcaggt tggcctgctg gctgacgagc
     3481 gctcgctcga gcgggaggaa atcggcggag tcggagaaga ccgcggtaaa gtgatcgcgg
     3541 cgaagcagtt cgatcgcctg atcgatggtc aacgcttcaa cgacgtcgca ccgagagcga
     3601 aggctctcaa tggccgggct gtttgccggc gaatgcccgg tcaagacgag aactttgtgc
     3661 cgcggttcgt ccaaagacac
//
LOCUS       DATIMH010001175         7617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4901842, whole genome shotgun sequence.
ACCESSION   DATIMH010001175 DATIMH010000000
VERSION     DATIMH010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7617
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4901842"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1400
                     /locus_tag="VLI90_20240"
     CDS             195..1400
                     /locus_tag="VLI90_20240"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSV16605.1"
                     /translation="MPPKRANSPPKPDHLRVAVRVAGWSQWQVQVIRGVQAYAHQQPR
                     WRLYVDGTPSAGSIFGRDLRWDGIITREQDMNALWRKLMRAGRTKIVSLTSAPVKGKP
                     LPTVRVGDHKTAQIAGRHLIAGGFRRLAYVGPLGWGRNDFRGQAIVAFANAEGVPCDI
                     FPGGAEVGDVDPWNFDSRISMNRLTRWIARLPKPVGIFTWNMTTARVAAETSKRARCL
                     IPEQVGIVAGDDEPTEAEHYERTLTGIVLPAERLGFEAAKLLDDLMSGGAAPKEPMLV
                     EPSGVTHVRQSSDVSMLPDRQVHPAVQFIREHASQPLSVPRVARAMRMSRSNLDLQFT
                     RVLGHTPRQEIALAHIERAKQLLLETNWPIERIAPRAGFGSWRQFYRTFVQREGLTPA
                     VYRARYHIG"
     gene            1410..2144
                     /locus_tag="VLI90_20245"
     CDS             1410..2144
                     /locus_tag="VLI90_20245"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HSV16606.1"
                     /translation="MSTDRTDSSRPAWEPIVVAHRGFWWLDLPENSRAAFARARDFDF
                     PAECDVQQTLEGEPIVIHDELLDRTTTGRGQISACSAESLRQLRLKHPRGLTEPPPML
                     RDVSNLVALVEIKPPDARSVVQRVIDIMRGQRWLLQSFDPRNIEHALEVDASTSIALL
                     VDSMNGFEPAISSRWTVHADHQLLDDRTVGTLHDHGLRVGAWTVNTEEEITRILPLRP
                     DVIISDIPIIVRQILVTSGVRVGEFK"
     gene            complement(2197..3831)
                     /gene="gltX"
                     /locus_tag="VLI90_20250"
     CDS             complement(2197..3831)
                     /gene="gltX"
                     /locus_tag="VLI90_20250"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSV16607.1"
                     /translation="MSNENVVTRFAPSPTGYLHVGGARTALFNWLFARHTGGRFLLRI
                     EDTDLARSTEDATRQLLEDLRWLGLNWDNEQLVYQSKRLDLYNKLIDSLVEKGQAYEA
                     WETPEELDAMRQQAQRAKRPFIYKRPTYTPDLLKRFRDEGRTAVIRFAMQAKEYRFHD
                     VTLGKDVVQTPYEVQDFVIRKGDGMPTYHFGVVVDDAEMGVTHVLRGLEHLLNTVNHI
                     ALQEALGYPRPIYAHLSVMLNPETGEKLSKRDRDRRIRKRVQEWMRSTKRTVEEVGAA
                     SGVVPSRLTEWLNSDTMQLDLSEQPKVMAVVGLTEGDLPEIMIHDFRRNGYVPEVLLN
                     FLALQGWSPGGDREQMTVQEMVELFSLERINSANPKFNREKLRSFSTDFFSKAPPQRL
                     VAAMRDYLSVNADSPLSQATDEQLAKLLHMNAGFHVLREVDEKSRFFFTPDERIEYAR
                     AAVDKVLLKNDRQGLNALTAVRPILAGVNDWTAANLEAAVKAYCEQSGLGLGKVAQPI
                     RVAVSGTAVSPPIFESLEFLGRDRTLARIDRCIANV"
     gene            complement(3948..>7617)
                     /gene="addA"
                     /locus_tag="VLI90_20255"
     CDS             complement(3948..>7617)
                     /gene="addA"
                     /locus_tag="VLI90_20255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HSV16608.1"
                     /translation="LVSAAAGSGKTTVLAERCAHLICDADEKCDVDDLLVVTFTEAAA
                     AEMRDRIHKSLAQRHARQPSDHTGRQLAMIDRASVGTLHAFCARLLRQHFHLLGLDPD
                     FLILDADEAELMQLEVARNMFADYYDMEGGGSDAFCKLVDCYADGTDERLVEKVIQAH
                     DTLCSVVDPTAWLNNARKRIAQAIDLPLHESDLGRAYIKTIRQYLEAMRTQCADAGKA
                     IKALKNFPFYVDQMRDLYRTIDHWLKVLSQHGLEMFVTEAATVELPPLPRVSNSVEGK
                     EAAKGRVDDIRKAMKEGHWRQCLAFSPEEWKEGLERIQPHADTFLSLVEDFGARYAKA
                     KDEEGGLDFSDLERFTLKALRDASEPDVLAPSPVAKMYHRQFKHVLVDEYQDINEVQD
                     AILTLISRECVASDRRRVVPNLFCVGDVKQSIYRFRLAEPARFLKRRDVYSEKDSHGT
                     VIDLQSNFRSRAPLLAVINAVFEKLMTADAADLNYDESQRLSPGAEFPASGDGAGHFS
                     GAPIELHVLPKDAGTSSGGDEESDDAALDRSEREATLLAHRILEMTGKGPGLRSTSDT
                     DVDSTPMHVFDSVAKSYRPIRYGDIVILLRSMRYKADRFAQKLRDAGIPVHSESATGY
                     FEATEVNDVLSLLKVLDNQRQDIPLAAVLRSPFARIPEAEDQLARIRLRYPGDPPVPF
                     HDAVAQYAAEQDDELAARLRDLLAQLAHWRRLARQRPLAELLWTIYTDTGYLAFCAGL
                     PNGEQRQANLIELHERARQFGSFRRQGLSRFLQFLDKLKSESDLGQASIASEAEDVVR
                     IMSIHRSKGLEFPVVMLPDLGKAFNLQDCQGSILLDRQAGLGLQVVDEERRIRYPSMA
                     STVVQQRLRQQSLAEELRVLYVAMTRAKEHLIMVGTCSEGACDKWTTQWANHEGPLPA
                     EAVLGARAMLDWIGPVSAVLARASTQTFELHAHTLEEVYAWSAAQQTSSGLTPAQAAL
                     AALRPLDPPPAESAAAREVIDRVSAPYAFTAFTKLRAAQSVTGQLLAATPASPGPKEP
                     TQASQLQTLDSVLPKPAFLVGDLPPDAVDRGSATHAVLERLDFADAADAGAIARQIDG
                     MVAARRLLAEHAKLVDVASILWLLQQDIGRMLRENAPRLLREVPVYLAADSAGAKDPL
                     DRAMIRGRIDVLVPVGDGYTLVDYKTDRVGGDALADRAKSYAAQLALYRDAIERITGK
                     RVTRTVIVFLQARETLDV"
BASE COUNT         1356 a         2697 c         2183 g         1381 t
ORIGIN      
        1 cacgccgggt tcggagcgta ctcgcgacga agcgagattc ttctgtacga gtaggaaaag
       61 catccgcatc gtcggagtat ccttggactt cgctcaggcc tccgaactcg gcgtgatgga
      121 ctcctgcgca tcccgccgcc gctggttcgt tcatccgcgc aatggtaaaa ccccaacgcg
      181 cggtcaccaa ccccatgccc cctaagcgcg ccaactcgcc gccgaagccg gaccacctcc
      241 gcgtcgccgt gcgcgtcgcg ggttggtcgc agtggcaggt gcaagtcatc cgcggcgtgc
      301 aagcctacgc ccaccagcag ccgcgctggc ggctctatgt agacggtacg cccagtgccg
      361 gttccatctt cggccgcgac ctgcgctggg acggcatcat cacccgcgag caggacatga
      421 acgcgctgtg gcgcaagctg atgcgcgccg gccgcaccaa gatcgtctcg ctcacttctg
      481 ccccagtcaa aggcaaaccg ctgcccaccg tgcgcgtcgg cgatcacaag accgcccaga
      541 tcgccggccg gcatctcatc gccggcggct tccgtcgcct cgcgtacgtc ggcccgctgg
      601 gttggggccg caacgatttc cgcggccagg ccatcgtcgc attcgccaac gccgaaggcg
      661 tgccctgcga catcttcccc ggcggcgccg aagtgggcga cgtcgatcca tggaacttcg
      721 attcgcgcat cagcatgaac cgcctcacgc ggtggattgc ccgcctgccc aaaccggtcg
      781 gcatcttcac gtggaacatg accaccgcgc gtgtggccgc cgagacgtcc aagcgcgccc
      841 gctgcctgat ccccgagcaa gtcggcatcg tcgccggcga cgacgaaccg accgaagccg
      901 agcactacga gcgcacgctg accggcatcg tgctgcccgc cgaacgcctc gggttcgaag
      961 ccgccaagct gctcgacgat ctgatgagcg gcggggcggc gccgaaggag ccaatgctcg
     1021 tcgagccgtc cggcgtcacg cacgttcgcc aatcatctga cgtctcgatg ctgcccgatc
     1081 gtcaggtgca cccggccgtg caattcatcc gcgaacacgc cagccaaccg ctcagcgtgc
     1141 cgcgggtcgc gcgggcgatg cgaatgtcgc gcagcaatct cgacctgcag ttcacccgtg
     1201 tcctcggcca caccccgcgg caggagatcg ccctcgccca catcgaacgc gccaaacaac
     1261 tgctgctgga aaccaattgg ccgatcgagc gaatcgcccc ccgcgccggt ttcggttcct
     1321 ggcggcagtt ttaccgcacg ttcgtccaac gcgaaggctt gactccggcg gtgtaccggg
     1381 cgcggtatca catcggatga tgacgcaaca tgagcaccga ccgtaccgat tcatcgcggc
     1441 cagcttggga acccatcgtc gtcgcccacc gagggttttg gtggttggac ctccccgaga
     1501 attcccgcgc cgccttcgcg cgggcaaggg acttcgactt ccccgccgag tgcgacgttc
     1561 aacagactct cgaaggtgag ccgatcgtca ttcacgacga gctgctcgac cggaccacga
     1621 cgggccgcgg gcagatcagc gcctgctccg ccgagtcgtt gcgccagctg cggctcaagc
     1681 acccccgggg gctcacagaa ccgccaccga tgctgaggga cgtctcgaac ctcgtggcgc
     1741 tggttgaaat caagccgccc gatgcgcgct ccgtggtcca gcgagtgatt gacatcatgc
     1801 gcggacaacg ctggctcctg cagtcgttcg acccgcggaa catcgaacac gcgcttgaag
     1861 tagatgcatc cacgtcaatc gctttgttgg ttgattcaat gaacggattt gaacccgcga
     1921 tcagcagccg ttggaccgtt cacgcggatc atcaactgct cgacgaccgc accgtcggca
     1981 cattgcacga ccacggcctt cgtgttggag cgtggaccgt gaacaccgaa gaggaaatca
     2041 cgcgaatcct tccgctccga ccagacgtga tcatctcgga cataccaatc atcgtgcgtc
     2101 agatcctggt gacatcgggc gtgcgagtcg gtgaattcaa gtagcacggg cgtctcgccc
     2161 gtgtccaagc actttggcac gggcgagacg cccgtgctac acgtttgcaa tacaccgatc
     2221 gatgcgcgcg agcgttcgat cgcgtccaag gaattcaagg ctctcgaaga tcggcgggct
     2281 caccgccgtc ccgctcaccg ccacgcgaat cggctgcgcg actttcccca accccagtcc
     2341 gctctgctcg caatacgcct tcaccgccgc ttccaggttc gccgcggtcc agtcgttcac
     2401 gccggcgagg atgggccgca ccgccgtcag cgcgttcaac ccctgccgat cgttcttcag
     2461 cagcaccttg tccaccgcgg cgcgcgcgta ttcgatccgc tcatccggcg tgaagaaaaa
     2521 ccgcgacttc tcatccacct cgcgcagcac gtgaaagccg gcgttcatgt gcagcagctt
     2581 cgccaactgc tcgtccgttg cctgcgacag tggtgaatcg gcattcaccg acaggtaatc
     2641 ccgcatcgcc gccaccagcc gttgcggcgg ggctttgctg aaaaagtccg tgctgaatga
     2701 cctaagcttc tcgcggttga acttcgggtt cgcgctgttg atccgctcga ggctgaacag
     2761 ctcgaccatc tcctgcaccg tcatctgctc ccgatccccg ccggggctcc agccctgcag
     2821 cgccaggaaa ttcagcagca cctccggcac ataaccgttc cggcggaaat cgtgaatcat
     2881 gatttccggc agatcgcctt cggtcaggcc gaccaccgcc atcaccttcg gctgctcgga
     2941 taaatccagt tgcatcgtgt cgctgttcaa ccattccgtc agccgcgaag gcacgacgcc
     3001 actggcggcg ccgacttcct caaccgttcg cttcgtcgat cgcatccact cctgcacgcg
     3061 cttgcggatc cgccggtcgc ggtcgcgctt cgacagcttc tcgcccgtct cggggtttag
     3121 catcaccgac aaatgcgcgt agatcggccg gggataaccc agcgcctcct gcagcgcgat
     3181 gtgattcacc gtgttgagca ggtgttcgag tccgcggagc acgtgcgtca cgcccatctc
     3241 cgcgtcgtcc acgacgacgc cgaagtgata cgtcggcatg ccatcgccct tgcggatgac
     3301 aaagtcctgc acctcgtacg gcgtctgcac gacgtccttg cccagcgtca cgtcatgaaa
     3361 ccgatactct ttcgcctgca tcgcaaagcg aatgaccgcc gtgcggccct cgtcgcgaaa
     3421 ccgcttcagc aaatccggcg tgtacgtcgg ccgcttgtaa atgaacggcc gcttcgcccg
     3481 ctgcgcctgc tggcgcatcg cgtccaattc ctccggcgtc tcccacgcct cgtacgcctg
     3541 acccttctcg acaagcgagt cgatcagctt gttgtaaaga tcgaggcgct tggattgata
     3601 aaccagctgc tcgttgtccc agttcaaccc cagccagcgc aggtcttcca gcagctgtcg
     3661 ggtggcgtct tcggtgctgc gggccaggtc ggtgtcttcg atgcgcagca ggaatcgtcc
     3721 gcccgtgtgg cgcgcgaaca accagttgaa caacgccgtg cgtgccccgc cgacgtggag
     3781 atagccggta ggcgacggcg cgaatcgcgt gacgacattt tcgttggaca tagagccccg
     3841 cgattatgcg aagcccgcgg acgatcgcaa ggcaccgccg cttgcggctt agcatgggtt
     3901 tctgattcga gaaagaaaag gatgctaagc cgcaagcggc gggggagtca tacgtccagg
     3961 gtttcccgcg cctgcaaaaa gacgatcacc gtccgcgtca cgcgctttcc agtaatgcgc
     4021 tcgatcgcgt cacgatacaa cgccaattgc gccgcgtaag acttcgcgcg atccgccagc
     4081 gcatcgccgc caacgcgatc agtcttgtaa tcgacgagcg tatagccatc gccaacggga
     4141 accagcacat cgatccgccc gcggatcatc gcgcggtcca gcgggtcctt cgcgccagcc
     4201 gaatccgccg cgaggtacac cgggacttct cgcagcaacc gcggcgcgtt ctctcgcagc
     4261 attcgcccga tgtcctgctg caaaagccaa aggatggacg cgacatcgac cagcttcgcg
     4321 tgctccgcca acaaccgccg cgccgcgacc atcccgtcga tctgcctggc gattgccccc
     4381 gcatccgcgg cgtcggcaaa atccagccgc tccaacaccg cgtgcgtcgc cgatccgcga
     4441 tcgaccgcgt ccggcggcag atcgcccacc agaaacgccg gcttgggtag caccgaatcc
     4501 agcgtctgta gctgcgacgc ctgcgtcggc tctttcggcc cgggcgacgc aggcgtcgca
     4561 gctaagagtt gaccggtcac tgattgagcc gcacgcaatt tcgtgaacgc cgtaaacgcg
     4621 tacggcgcac tgactcgatc gatcacctcg cgcgccgccg ccgactccgc cggcggcggg
     4681 tccagcggcc tcaacgccgc cagcgccgcc tgcgccggcg tcaacccgct cgatgtctgc
     4741 tgcgccgccg accacgcgta cacctcctcc agcgtgtgcg cgtgcagctc gaacgtctgc
     4801 gtcgaagccc gcgccagcac cgccgacacc gggccgatcc agtccagcat cgcccgcgct
     4861 cccagcaccg cctcggccgg cagcgggcct tcgtggttcg cccactgcgt cgtccacttg
     4921 tcgcacgcgc cttcgctgca cgtgccgacc atgatcaggt gttccttggc gcgcgtcatc
     4981 gcgacgtaca gcacgcgcag ttcctcggcc agcgattgct gccgcagccg ctgctgcacc
     5041 acggttgatg ccattgatgg atatcgaatt cgccgttctt catcgaccac ctgaagccca
     5101 agcccagcct gccgatcgag caggatcgac ccctggcaat cctgcaggtt gaacgccttg
     5161 cccagatccg gcagcatcac caccggaaat tccaaaccct tggaccggtg gatgctcatg
     5221 atgcgaacga cgtcttccgc ttccgacgcg atcgacgcct ggcctaggtc cgattcgctt
     5281 ttgagcttgt cgaggaactg caggaaccgg ctcagtccct gtcggcggaa tgagccgaac
     5341 tggcgcgcgc gttcgtgcag ttcgatcagg ttcgcctgcc gctgttcgcc attgggcagg
     5401 ccggcgcaga acgcgagata gcccgtgtcg gtgtaaattg tccacaacaa ttccgccagc
     5461 ggccgctgcc gcgccaaccg ccgccaatgc gccagctgcg ccagcagatc gcgcagccgc
     5521 gccgccagct cgtcgtcctg ctcggccgcg tactgcgcca cggcatcgtg aaacggtacc
     5581 ggcggatcgc ccgggtatct gagccgaatc cgcgccagct gatcttcggc ttcgggaatc
     5641 cttgcgaacg gactgcgcag caccgccgcc agcggaatgt cctgccgctg gttgtccagc
     5701 accttaagca gcgacagcac gtcgttcact tccgttgctt cgaaataccc ggtcgcgctt
     5761 tcgctatgca ccgggatgcc cgcgtcgcga agcttttgcg cgaatcggtc cgccttgtac
     5821 cgcatcgacc gcagcaggat gacgatgtcg ccatagcgaa tgggtcgata agacttcgcc
     5881 acgctatcga acacatgcat cggcgtcgaa tcgacgtcgg tgtcggaggt acttcgaagc
     5941 cccgggccct tgccggtcat ttcgaggatg cgatgcgcca gcagcgtcgc ctcgcgctcc
     6001 gaccgatcca gcgccgcatc gtccgactct tcatcgccgc cgctcgatgt ccccgcatcc
     6061 tttggcagca cgtgcagttc gatcggcgcg ccgctgaagt gccccgcgcc atcgccgctg
     6121 gcaggaaact ccgcccccgg cgacagccgc tgcgattcgt catagttcaa atccgccgcg
     6181 tccgccgtca tcagcttttc gaacaccgca ttaatcaccg ccagcagcgg cgcccggctt
     6241 cgaaaattcg actgcagatc gatcaccgtg ccgtggctgt ccttctccga atacacgtcc
     6301 cgccgcttga gaaatcgcgc gggctccgcc aggcgaaagc gatagatgct ctgcttcaca
     6361 tcgccgacgc agaacaaatt gggcaccacc cgacgtcgat cgctcgccac gcactcccgg
     6421 ctgatcagcg tcaggatggc atcctgcact tcgttgatgt cctgatattc atccaccagc
     6481 acgtgcttga actgccggtg atacattttg gcgacgggtg aaggcgcgag cacgtccggc
     6541 tcgctcgcgt cgcgcagcgc cttcagcgtg aaccgctcca gatcggaaaa atccaacccg
     6601 ccttcttcat ccttcgcttt ggcatatcgc gcgccgaaat cctcgaccag cgacaaaaac
     6661 gtatcggcgt gcggctggat tcgctcaagg ccctccttcc actcctccgg cgaaaacgcc
     6721 aggcattgcc gccagtggcc ttccttcatc gccttgcgaa tgtcatcgac ccggcccttg
     6781 gccgcctctt tgccttcgac gctgttcgac acgcgcggca gcggcggcag ctccacggtg
     6841 gccgcctccg tcacgaacat ttccaggcca tgctggctta gcaccttcaa ccagtggtcg
     6901 atggtgcgat atagatcacg catttgatca acatagaacg gaaaattctt cagcgccttg
     6961 atcgccttgc ccgcgtcggc gcactgcgtg cgcatcgctt ccagatattg ccgaatggtc
     7021 ttgatgtacg cgcgacccag gtcgctctcg tgcagcggca ggtcgatcgc ctgcgcgatc
     7081 cgcttgcgcg cgttgttcag ccacgcggtg ggatccacca cgctgcacag cgtgtcgtgc
     7141 gcctggatca ccttctcgac cagacgctca tcagtgccgt cggcgtagca gtcgacgagc
     7201 ttgcagaacg cgtccgaccc gccgccttcc atgtcgtaat agtcggcgaa catgttgcgc
     7261 gccacttcca gctgcatcaa ttccgcctcg tcggcgtcca ggatgaggaa gtccgggtcc
     7321 agccccagca gatgaaaatg ctgccgcagc aaccgggcgc aaaacgcgtg cagcgtgccg
     7381 accgatgcgc gatcgatcat cgccaattgc cgcccggtgt gatcgctggg ctggcgcgcg
     7441 tgccgctgcg ccagcgattt gtgaatacga tcgcgcatct ccgccgccgc cgcttcggtg
     7501 aacgtgacga cgagtaggtc gtccacgtcg catttttcat cggcgtcgca gatcaggtgc
     7561 gcgcagcgct cggctagcac ggtcgtcttc cccgaccccg ccgccgccga gactaaa
//
LOCUS       DATIMH010001176         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3745019, whole genome shotgun sequence.
ACCESSION   DATIMH010001176 DATIMH010000000
VERSION     DATIMH010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3745019"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..3551)
                     /locus_tag="VLI90_20260"
     CDS             complement(84..3551)
                     /locus_tag="VLI90_20260"
                     /inference="COORDINATES: protein motif:HMM:NF042646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6603 domain-containing protein"
                     /protein_id="HSV16609.1"
                     /translation="MDGNDTLGALAEAVGRAVGPLVAAFESGDAMRDFLEDLGWNFDV
                     APPAINVLQTSVNNLASLLDAGAVDSSKVPQLLDLVASLYHGISDLESASGLATDFAQ
                     EFPRQLVDYLFVEYLLNELPKWGYLLLLLGLIRLEDTPAQNGRPAFTRRVFAIEDLGT
                     FMADPLTYLQNGYRWNQSSFAGAQLLQNMREFLAAWNTEARLQIADSGSLSVLNAGAS
                     APADASRVSLRLVFIEHSTDPETFAAGVGMFLLPETPNAKPGFALLPYTTVGFSDDVQ
                     LTSGLTLSFSSTVDLTGGVGAFVRPGQDVQLTAGLKTGAPTPVTGSLTVALKLGDPND
                     PTLLLGDPSGSRFECGGVSVTGGARFDSSAKREVFTEFALQAGKIVVQPSSGDADSFV
                     MSALPPDGLQMAVPLTVGFSTTRGVYFGSSGTFEINVPTHLQVGPVEIDAGTIAVRFE
                     NGGLPIDVSADFTLDLSVLKASFEKVGFTAAITFPKDRKGNLGTVDLSLGFKVPAGVG
                     IAVDAGPVSGGGFLSFDAPNHRYFGALELSVYSVSVKAFGVIETKLPTGPGYSFLIII
                     SAEFTPIQLGFGFTLNGVGGLIGINRSVDSAGLLTMIKTGQLDNVLFPEDVVDNAPAI
                     LNDVETLFPPTPGHYVFGPLAKIGWGTPTLVDGELGLIFEKPGPVLTVIGTIHAALPT
                     KDEAVVELNLDVGGVLDFPNKKFSLDASLHDSRVGDFPITGDMAMRMNWGQNPNFALS
                     VGGFNPHYNPPAGFPKLKPVTVDLGSNGNPSITLAGYMALTSNTAQVGALLQAHISKD
                     GASLSGFLGFDALFVFSPFSFEASIEGGVSVSIDGYGFSLDFHGTLSGPSPWHLAGKV
                     CVSLLFWDACADFSTTIGGTSQPTLPGVDPWTGSLPLNADGTQTVPGLQPALSNPANW
                     GGNPPAGSFPAVTLVAAPKGAPAPVDPLGSATFRQKAVPLDLTITSFAGTQPSPQRNL
                     DIAKVVVGTQTITEPKRVSDYFAPGQYQKMKDADKLSSQSFVQLPAGFTISSDAVTFG
                     PAVSEGLVYTDFTFAPPVGSPPVITMDAYAPTQTHVTTVTGRSAVATKGLRAAALAAF
                     VDPKAAPLVTLANELYTIANITNLLGATGFATNVPRPVATSALDLAAATNVIARQTLQ
                     VAPAFFVVH"
     gene            complement(3563..>3759)
                     /locus_tag="VLI90_20265"
     CDS             complement(3563..>3759)
                     /locus_tag="VLI90_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16610.1"
                     /translation="NGERIVYTMLVPITNPDPGPKPVANLLFNFYPNQGSPLTAPPPQ
                     LVETKGTLFATVKAPVPVSP"
BASE COUNT          714 a         1229 c         1187 g          629 t
ORIGIN      
        1 aatgtcccgg ccacccgcca gcgcaaacag gtgcggtcca tcctcgccca caatggccgg
       61 atccggggtg ccgtggcatc ccctcagtgg acgacgaaga aggcgggcgc cacctgcagc
      121 gtctgccgcg cgatcacgtt cgtcgccgcg gcgaggtcga gcgcggaggt cgcgaccggc
      181 cgggggacgt tcgttgcgaa cccggtcgcg cccagcagat tggtgatgtt ggcaatcgtg
      241 tagagctcgt tggccagggt gaccagcggc gccgccttgg gatcgacgaa ggccgccaac
      301 gcggcggcgc gcagcccttt ggtggcaacg gcgctgcgcc cggtcaccgt cgtcacgtgc
      361 gtctgcgtcg gcgcgtaggc gtccatcgtg atgaccggcg gtgaccccac cggcggcgcg
      421 aaggtgaagt cggtgtagac cagcccctcc gacacggccg ggccgaacgt caccgcgtcg
      481 ctggagatgg tgaagccggc gggcagctgc acgaacgact ggctcgacag cttgtcggcg
      541 tccttcatct tctggtactg ccccggcgcg aagtagtcgc tcacccgctt cggctcggtg
      601 atcgtctgcg tccccaccac caccttggcg atgtccaggt tccgctgcgg cgacggctgc
      661 gtccccgcga agctggtgat cgtcaggtcg agcggcaccg ccttctgccg gaacgtcgcc
      721 gaccccagcg gatccaccgg cgccggcgct cccttgggcg ccgccaccag cgtcaccgcc
      781 gggaacgatc cagccggcgg gttcccgccc cagttggccg ggttggagag cgccggctgc
      841 aaacccggaa cggtctgcgt gccgtcggcg ttcagcggca acgagccggt ccacggatcg
      901 acacccggca acgtcggctg cgacgtgcca ccgatggtgg tggagaagtc ggcgcaggcg
      961 tcccagaaca gcaggctgac gcagaccttg cccgccaggt gccagggcga tggcccgctg
     1021 agcgtcccgt ggaagtcgag cgagaacccg tagccgtcga tgctgacgct gacgccaccc
     1081 tcgatgctcg cctcgaacga gaacggcgag aagacgaaca gcgcatcgaa ccccagaaac
     1141 cccgacaggg acgccccatc cttggagatg tgcgcttgca acagcgcccc cacctgcgcc
     1201 gtgttggagg tgagcgccat gtaccccgcc agcgtgatgc tggggttgcc gttcgagcca
     1261 agatcgacgg tcaccggctt gagcttgggg aagccggccg gcggattgta gtgcgggttg
     1321 aagcccccga cggagagcgc gaagttgggg ttctgccccc agttcatccg catcgccatg
     1381 tcgcccgtga tcgggaagtc ccccacccgc gagtcgtgca ggctggcgtc cagcgagaac
     1441 ttcttgttcg gaaagtcgag cacgccgccc acgtcgaggt tcagctcgac gaccgcctcg
     1501 tccttcgtcg gcagcgccgc gtggatggtc ccgatgaccg tcaacaccgg ccccggtttc
     1561 tcgaagatga ggcccagctc gccgtcgacc agcgtcggcg tcccccaccc gatcttggcc
     1621 agtggcccga acacgtagtg accgggggtg ggcgggaaca gcgtctccac gtcattgagg
     1681 atcgccggcg cgttgtcgac cacgtcctcg gggaagagca cgttgtccag ctgccccgtc
     1741 ttgatcatcg tgagcaggcc ggcggagtcg acgctgcgat tgatcccgat gagcccgccg
     1801 acgccgttga gcgtgaaccc gaaccccagc tgaatcggcg tgaactccgc cgagatgatg
     1861 atcaggaacg agtaacccgg ccccgtcggc agcttggtct cgatgacccc gaacgccttg
     1921 acggacacgc tgtaaacgga aagctcgagc gcgccgaagt agcgatgatt gggagcgtcg
     1981 aacgacagga acccgccgcc cgagaccggc ccggcgtcga cggcgatgcc cacaccggcg
     2041 gggactttga aacccaggga cagatccacc gtccccaggt tccccttgcg gtccttggga
     2101 aacgtgatcg ctgcggtgaa cccgaccttc tcaaaagacg ccttcagcac tgacaggtcg
     2161 agcgtgaaat cagcggatac atctatggga aggccaccgt tctcgaatcg aacagctatg
     2221 gttccagcgt cgatctcgac cggtccgacc tgaaggtgcg tcggtacatt gatttcaaag
     2281 gtgccggagc ttccaaagta cacgccccgc gtcgtcgaga agcccaccgt gagcggcacg
     2341 gccatctgaa ggccgtccgg aggcagggct gacatcacga aagaatccgc atcgcccgag
     2401 ctcggctgga caacgatctt tcccgcttgc aaggcgaact ccgtgaacac ctcacgcttt
     2461 gcgctcgagt cgaatcgcgc acccccggtg accgagaccc cgccgcattc aaatcggctc
     2521 cctgaggggt cacccaggag cagagttgga tcgttcgggt cacccagctt gagagcgaca
     2581 gtcagactcc cggtgactgg cgttggcgca ccagtcttca gacctgctgt cagctgaaca
     2641 tcctggcccg gtcgtacaaa cgcgcccacg ccccctgtca gatccacggt gctagaaaag
     2701 ctgagcgtca gaccacttgt gagctgaacg tcatcactga atccgaccgt cgtgtatggc
     2761 aggagtgcga aacctggctt cgcgttcggc gtctccggga gcagaaacat gccgactccc
     2821 gctgcaaacg tctcaggatc ggtgctgtgc tctataaaca caagccgcag gctcactcgc
     2881 gaagcatcag cgggcgccga cgcgcctgcg ttgagaacac tcagcgaacc gctatcagcg
     2941 atttgcagcc gcgcttccgt attccaggcg gccaagaact cgcgcatgtt ttgaagcaac
     3001 tgggcgccgg cgaagcttga ctggttccac ctatagccgt tctggaggta ggtcaacgga
     3061 tcggccatga acgtgccgag atcctcgatg gcgaataccc tacgcgtgaa tgccggtcgg
     3121 ccgttctggg ccggcgtatc ctcgagacga atcagcccca gcaagagaag caggtatccc
     3181 cacttcggca attcgttcag gagatattcg acgaacagat agtcgaccaa ttgccgagga
     3241 aattcctgcg caaagtccgt cgcgagcccg ctcgccgatt cgaggtccga gatcccgtga
     3301 tacagtgacg cgaccaggtc gagcaactgt ggcaccttcg acgaatcgac tgcgcccgca
     3361 tcaagcaaag acgccagatt gttcacggac gtttgaagga cgttgatcgc aggtggagca
     3421 acgtcaaagt tccagccaag atcttccaga aagtcgcgca ttgcgtcgcc cgattcgaac
     3481 gccgcaacga gggggcccac cgcgcgtccc accgcttcgg ccagcgcacc cagcgtgtca
     3541 tttccgtcca tttgcgcttt tcctagggag agacagggac cggcgccttg acggtagcga
     3601 atagcgtacc ctttgtttct accaattgcg gcggcggggc agtcaacggc gatccttggt
     3661 taggatagaa attgaacagc agattggcga ccggcttggg cccgggatcg gggttggtga
     3721 tcggtaccaa catcgtgtac acgatgcgct caccgttgc
//
LOCUS       DATIMH010001177         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2590295, whole genome shotgun sequence.
ACCESSION   DATIMH010001177 DATIMH010000000
VERSION     DATIMH010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590295"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VLI90_20270"
     CDS             <1..1011
                     /locus_tag="VLI90_20270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSV16611.1"
                     /translation="IQMIRRNVELEARLIDDLLDLTRIAKGKLQLSLDTVDAHASLNS
                     ALDICRADLAEKGVHIGVDLSATQHHVRADPARIQQVFWNLIKNAVKFTPTGGQVTVR
                     TRNEPDGRLRVDVTDTGIGIEPENLKRIFFAFEQAEQSITRQFGGLGLGLAISRSLME
                     MHGGTLSADSLGKDRGSTFTARLHTVAAPIGAPALKPGKSPAQGQSLGTAAAAARSRR
                     ILMVDDHEDTSRAMKRLLERLGYEVHTKHTVREAYDAANASNYDLLISDIGLPDGSGV
                     DLIRQLRQNANPIRALALSGFGMDEDIRRSKEAGFQDHLTKPVNFSKLQEVIAELIES
                     GG"
     gene            complement(1181..>2762)
                     /locus_tag="VLI90_20275"
     CDS             complement(1181..>2762)
                     /locus_tag="VLI90_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16612.1"
                     /translation="HQVLPAGWLQTSPTHDLGRHVHLGQGASVKGQDFGDRPTALQWG
                     SYAGNAQHTGISPFQSQSLDQIKWQTAVDQQPQIVDNDIFIHYGEPLFTHDNVMILPV
                     KTQADGGFELQGRRGSDGKLLWTVKSDYVQPPHAWGLPFSPALTGNDRVYFPGAGGTI
                     HYIDHANLPGAKTVHSLSYYGLANYTAHKSALDGTTFVDTPLTLGPDGGVYYGVQVTG
                     TNPLNLHGGLARVAGFGAPAFASAASASGSLQMDKVAQNCAPALSNDGKIVYVTVNQT
                     NFIGVGAGILASDYLLALDAETLKTVGKTQLIDPASGSFAQIPDISTATPTVGPDGDV
                     YYGVLDPGNDNHDRGFLLHFNRTLAQSKTPGAFGWDDTASVVPASIVPSYHGSSKYLL
                     MTKYNDYAGLGGTGVNKLAVLDPNATETDPISGATTMKEVLTIAGVTPDKEFPELPGA
                     VREWCINTAAVDPYTDSILAGSEDGKLYRWNLASNTFSQVIKLTDGVGEAYTPTAVGP
                     DGTVYAINNGTIFAVSSR"
BASE COUNT          503 a          879 c          894 g          486 t
ORIGIN      
        1 atccagatga tccgccggaa cgtcgagctg gaagcccggc tcatcgacga cctgctggac
       61 ctcacgcgca ttgccaaggg caaactgcag ctgagcctcg acaccgtcga tgcgcacgcg
      121 tcgctgaaca gcgcgctcga catctgccgg gccgacctgg ccgagaaggg cgtgcacatc
      181 ggcgtcgatc tgtcggccac ccagcatcac gtgcgggcag atccggcgcg catccagcag
      241 gttttctgga atctgatcaa gaacgccgta aagttcacac cgaccggcgg acaggtgacg
      301 gtgcgcacgc gcaacgagcc ggacggccgg ctgcgcgtgg acgtgaccga caccggcatc
      361 ggcatcgaac cggaaaacct caagcgcatc ttcttcgcct tcgaacaagc cgagcaatcg
      421 atcacccgcc agttcggcgg ccttggcctg ggattggcca tctcgcggtc gctgatggaa
      481 atgcacggcg gcacgctcag cgctgacagt cttgggaagg accggggttc gacgttcacc
      541 gcgcgcctgc acacggtggc cgccccgatc ggcgcgcccg cgctgaagcc ggggaaatca
      601 ccggcccagg gtcaatcgct cggcaccgcc gcggccgccg cgcgcagccg gcgcatcctg
      661 atggtggacg atcacgaaga caccagccgg gcgatgaagc ggctgctcga gcggctgggt
      721 tatgaggtgc acaccaagca cacggtgcgc gaggcgtacg acgccgccaa cgcgtcgaat
      781 tacgatctgc tgatcagcga catcggcctc ccggatggca gcggggtgga cctgatccgc
      841 cagctccgcc agaacgcgaa tccgattcgc gcgctggccc tgagcggctt cggcatggac
      901 gaagacatcc gccgcagcaa ggaagcgggg tttcaggatc acctgaccaa gccggtgaac
      961 ttcagcaaat tgcaggaagt gatcgcggag ctgatcgaaa gcggcgggtg atcgtttcca
     1021 tctcgacgag tgatccacgc cgggttcgga gcgtacgcgc gacgacccgg atgcttctcg
     1081 cgccgcggaa tccgggtcgt cggagtacct ccgaacccgg cgtggtaggt ctgtaaccca
     1141 atgaggacaa aaagcccgcg cggcgtggat tcacatcaca tcagcgcgat gacaccgcga
     1201 agatcgttcc attattaatc gcgtagaccg ttccatccgg tcccaccgcc gtgggcgtgt
     1261 acgcttcgcc gacgccgtcc gtcagcttga tcacctgcga gaacgtgttg ctcgccaggt
     1321 tccagcgata cagcttgccg tcttcgctgc cggccaggat ggaatccgtg taaggatcca
     1381 ccgcggcggt gttgatgcac cattcgcgga cggcgccggg cagttccggg aattctttgt
     1441 ccggcgtgac gccggcgatc gtcaggacct ccttcatcgt cgtcgcgccg gagatcggat
     1501 cggtctcggt cgcgttcgga tcgagcaccg cgagcttatt cacgccggtg ccaccgaggc
     1561 cggcatagtc attgtatttc gtcatcagca aatacttcga cgagccgtga tagctcggca
     1621 cgatcgacgc ggggacgacg gaggcggtgt cgtcccagcc gaaggcgccg ggggttttgc
     1681 tttgcgccaa agtgcggttg aagtgcagga gaaacccgcg gtcgtggttg tcattgccgg
     1741 ggtcgaggac gccgtagtag acgtcaccat cgggaccgac cgtcggagtg gcggtggaga
     1801 tgtcggggat ctgcgcgaac gaaccgctgg cggggtcgat cagctgcgtc ttgccgacgg
     1861 tcttcaatgt ttccgcgtcc agcgccagca ggtaatcgga cgcgagaatg ccggcgccga
     1921 cgccgatgaa gttcgtttga ttgacggtga cgtagacgat cttgccgtcg ttgctgagcg
     1981 ctggcgcgca gttctgcgcg accttgtcca tctgtaagct gcccgatgcg ctggcggccg
     2041 aggcgaaggc gggcgcgccg aatcccgcca cgcgcgccaa tccgccgtgc aagttgagcg
     2101 gattggttcc cgtcacctgc acgccataat acacgccgcc gtcgggtccg agcgtgagcg
     2161 gcgtatccac aaacgtcgtc ccgtccagcg ccgacttatg tgcggtgtaa ttcgccaggc
     2221 cgtaatacga cagcgaatgc accgtcttcg cgccgggcag gttcgcgtga tcgatataat
     2281 gaatcgtccc gccggcgccg gggaaataga cgcggtcgtt gcccgtcaac gccggtgaaa
     2341 acggcaaccc ccacgcgtgc ggcggctgga cgtaatcgct cttcacggtc cacaacaatt
     2401 tcccatcgct gccgcgccgg ccttgcagtt caaagcctcc gtccgcctgc gttttcaccg
     2461 gcaggatcat cacgttgtcg tgcgtgaaca gcggctcgcc gtagtggatg aagatgtcgt
     2521 tgtccacgat ctgcggctgc tgatcgacgg cggtctgcca cttgatttgg tcgagcgact
     2581 gcgactggaa cggcgaaatg ccggtgtgct gcgcgttgcc cgcgtagctg ccccactgca
     2641 atgccgtcgg tcgatcgcca aagtcctggc ccttaacgct ggcgccttgg cccagatgca
     2701 cgtgccggcc gagatcatgc gtcggcgacg tctgcaacca acccgcgggc aggacctggt
     2761 gc
//
LOCUS       DATIMH010001178        14885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1146400, whole genome shotgun sequence.
ACCESSION   DATIMH010001178 DATIMH010000000
VERSION     DATIMH010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 14885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14885
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146400"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VLI90_20280"
     CDS             complement(<1..872)
                     /locus_tag="VLI90_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HSV16613.1"
                     /translation="MKISAWCYPLMTAALVLGSANVHAQETTLPPKLDESVDRGLTFL
                     SRQQNADGSIDGGGPRVAMTGLSILSMLASGHTPDVGRYGLTVRRAIDFELRQMPENG
                     YIGKVDGSRMYGQGIVTLALAESLGVETTEAGRQRIRVAIGRAVKVILAAQDVPKDVN
                     SAGGWRYEPTSVDSDLSMSAWNALALRAAADVGIDVPRERAQRAVAFVLKCYRADQNG
                     FAYQPGQNATESMTAVGVLSLYLLNAGENDVLTRATPQLAVKLPNDQTRYAYYAMYYV
                     TQAAFQAGEPVWPIV"
     gene            complement(869..4030)
                     /locus_tag="VLI90_20285"
     CDS             complement(869..4030)
                     /locus_tag="VLI90_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16614.1"
                     /translation="MIDELPNPIRIYVTDYVARRRRVVIAKAIARAATVFIGWALLMC
                     LADRALNLRAAARLAALLAGVAAFVVLVLPQLRISFRRQFDWLSAAAEIDQLHPILGQ
                     RLITVASQYNEPTSHRGSPQILQHLAQGVAADVANDRGGRLVDGTPVWRAIIALTMLL
                     ALTVALCFVRPLGLPRLLSRFAQPLADVPPVTTVQLSVTPANIDVLEGHPVRILASTE
                     RLDTNSVPIVHLSDDGQSWSTTPATQLRPREFAYSIPAADHDLSYFVTAGDAITPIYT
                     VHVLRPPAVVDFRIRYTYPAYTRLPPRTVSNTDGAIEAPVGTTAMVSIHGNQPLKAAA
                     MLIGSTTLSLNPTAEPSIQQAQLSVRQSEPFTLRMTARNGVDGIGLSRGSIRAMPDHP
                     PHVRLVPPRPKIAAVRSDDVMTLRYEAEDDYGVEVLMAHVRRVSAAAPGISGDVTIPI
                     SGDARRLQGEQRLDLAKIELGRLAVGDVIYVVVEARDHLGQSALSEPWHLLITNEPTD
                     PAAPARLAALAAAGELAGHLHDELVAAESDQPERRSHRLADALQSASSARDALIKAVI
                     GDSSQSMSNALSNLVDTVETCAAVVDGAMSGNAPESEIKVKLADTADAITHVHESIVA
                     LTNAERAAAVVAERRMLSELAANGRTPELAAVQHQIELTVEDSLRPMGVHVHAADLDQ
                     QLIQRSAAGSAFLQGQHRPDFLAASRAFAAGLGRQNDWPPPLGRRLAAGALVEAARAD
                     GDLIRANDLQLAANAARRLSALQSLPATTQPSGDPRTAFPAAFEAMEHDVLDADPTAR
                     ATAQRARELMRRWAGEDESAATDEPLDAADRLNAAMAANAAAARRQYEQSQKLDDELR
                     RARTQPAMPASISADPSAARDLQQVMTAAKQIDQLIAEQARVRAAAAPADAPAAQGQR
                     SITQQIEQLQQHGLINREPLQQARAASEIAAAALAKRPPDLNEARTRQAEMSSLLGQA
                     WDAAIHEAARARLSLTPSIANLFTPGEPLRSAVLSQSPAWGRPPEPVGSSQAPLPRDE
                     PAGYESALKAYFEVLSGSSGAKP"
     gene            complement(4039..6690)
                     /locus_tag="VLI90_20290"
     CDS             complement(4039..6690)
                     /locus_tag="VLI90_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16615.1"
                     /translation="MLAALYWYSPQRLPIVLAILAGAVALTWWLYPSQVSSLSRRWRW
                     LLPMLRAGAMGALAISLLQPSVIRPRTDAERGPLLVLVDNSRSMSVSDTGRSPAQLIA
                     LASALGQLPSGVRDETAASLQADADQIATLADAIARQRSELDYARLAGRGVAAAQARL
                     DENVEQLRALARSAAGKLSTLPSAGVAHEAMTLLSQSARPDFDAWLEALPSRVQSAVA
                     AIEHARTLADEQLFRDDPAVHEVCQDLSHQSRLDLAITAITGDGGTAAKLAAVMPVLY
                     YAVGDRVTPFASDQTTATPSHPIEADGAVSNLTGAVRAVLDYMQGTPVRGVVLLSDGR
                     PVGGDTTVPSTLASGGVPVYAVSVAPPQALKDAAVLRISAPSSTFMGETITVRADVRA
                     TGVPAGTAAEVTFEVGGANESKQTRQIALAGDAVTTVEFTHEVTATPAAGQLLTVSIS
                     PLAGEATTENNRAQRWLKVIQSDPIRIAAFAGSAGWDFQYVCSALSREPWCRLADGIL
                     SNGAPLPLSPRDILRQDVIMLFDVAVDSLDAAQWGALSQLVKDRGGSVILVAGDAHLP
                     AEYLQHPAASALLPIIGPAPAWHISSGDRPGVHVIPSPGTNRVDALRLASGNEPMDRR
                     WQQLPGMYRFLPISPLKPDARSLLMDGETAQPVLTESRIGFGRSFILGINETWRWRYK
                     VGGRDQQRFWLQLLRYAAEEPYDVVEGPVALDADALEVTPSTPVRVRARVLGEDELPA
                     KGARAVISVLRDRRIVRSQVMASADGRFTTTLVDLPEGSYQLQLDAGPTLPHPAVPLR
                     VQRSYQAEMADLTVDDQLLRQLARASGGEFLRLDQIDTLPRRLTEIQPQAAGFVERTL
                     WDSPQLYAFVVGCLGVEWALRKRFGLA"
     gene            complement(6711..8876)
                     /locus_tag="VLI90_20295"
     CDS             complement(6711..8876)
                     /locus_tag="VLI90_20295"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HSV16616.1"
                     /translation="MFLNPLMLAGVGGAAVPVVLHLLSRARYRTVDWGAMMFLTGVDA
                     RQRRSTRLKQWSLLLVRSLIVAALALALARPAISSHNANLGGNGTATAVIILDDSASM
                     ACVENGRSRLDLARQAALQILSAMNRGDQVAFIPTGTPLAPADRAPTSDLQSVAARIA
                     AVRPGFGQADFVNSLSRAADVLERYERVNREIYIITDRQAISWRRVASDPFRRAWHAR
                     FPGGPAPRTTVITVGGDTANNLVVESLDVLNPPIIKDQPTDLEIHVRNIGAQPHSNIP
                     MTVSVGGRAVAELPVTLAPDSTAVLHATIRAPESGSRLITAALKAGGLTADNRLDRAV
                     DVYAPLKVLIVGDEDADPGKTQPSRWSAASLIRLALTPNASSGAPGGADPAIVKVVVP
                     SKFAAADLDGVDVVILANIDRFGSSQARDLEQFVFSGGGMLIAPGNVSRVDEYNNVLF
                     RFGAGILPASLSPPTPLATTGTLLVDVDLTHPIFGFLNGHTDILGTAAVLRYFPATPR
                     TADVRVLGSYASGYPFLIEGAAGRGRVLLVTTSLDADWTTLPQTSFYLPFLQSAVRYL
                     AGNGPNNRNLAPSQPIIASIAEPVEEHSANLRLPNGETEQLPVLRLEDRTEIRFNRTD
                     EPGVYRLRYRSEGKERLLHYVIVSPRAESDLTPMNDAQWAAVTDAVGLQHIDAGETPV
                     VSTVARDRAGRELWMPVMAGVIVLAAAEMLLARRWSSEP"
     gene            complement(8907..9800)
                     /locus_tag="VLI90_20300"
     CDS             complement(8907..9800)
                     /locus_tag="VLI90_20300"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSV16617.1"
                     /translation="MSLVSRYLQPSLVERLNHMQLTARSVVQGSTSGLHKSLLKGASV
                     EFRQHRPYVPGDEPRRLDWRLLARTDRPFVKEYDEETNLRCVIVLDRSGSMAYAGGGR
                     ATKFDYAARLVASLAYLMLAQTESVGLSVAGKSLESWLPPASGHSQLSRVIDMLERAT
                     PAGPAAVSAAINQVADRIDRRLLVIIVSDLFEPAPAIRSALARLRYARHETICLRVLD
                     PDEQEFPFRTWSHFRGFEHEPAYLCEPALVRQSYLANFNRHRDELEEACRSLRSECHT
                     FLTNRPMIDLLTKMLHRRAMV"
     gene            complement(10131..12917)
                     /locus_tag="VLI90_20305"
     CDS             complement(10131..12917)
                     /locus_tag="VLI90_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16618.1"
                     /translation="MTRRLSTPLVALLVPVLSLIAAAVVHAEVYTNVPLKELTFPNGG
                     VDHAPTSGPSVANRNGSIDARIVMDNGAEAYFAGDDGVWITQRLADLAPRRIVIRSDA
                     AGQLAGTIYGANDDNTAVNAVRFTVNSADHPGTRQDFLKARFAYYQHLQQSSLPGAAY
                     FRQRVRAARQELGKDAPPELPEQLAQSNHDDELDQTFGFASGGRAVSENLQLDRALPS
                     TTQPAAVDVKTADLQGVATQPFDWKPLVKDLHPQPDALAAIIPADQHAIFFPSFAACM
                     ALADRADQQLSPFISTIESAAGDARTRQRYERQLCLSMTQLGRLLGPSLIDSVAITGS
                     DPYWRVGSDLAVIFQAKDAATLNRLIAAQQMMRRASEPEASPVNGEVAGISYSGLVSG
                     DRGVCSYLATIGPAVVVTNSLVQLEKLASVQQAKAAALSSTPEYTFFRDRYRLGDEKE
                     TALLVLSDQTIRRWCSPRWRIGDSRRTRAAAVMSDLQAEYARDIAMGPIKRQIVTPNR
                     FVPGLDRMSLHTGGVFSDTFGSLDFMTPIAELNLDTVTREEADAYDRWRSGYERNFRW
                     FDPIAIRISLGATSIGVDTTVMPLMVSSDYQDVVELSAGAKLTADSADPHYAMAQIAM
                     AFNPKSKQLINLSNIAQSFGGGAAGIDPLAWLGHSASLYGDDDGFWDELAKADQADEF
                     IRHQVARLPVALYVESSSPLKLAAFLTALHGLADQSAPNLTTWETLKHGDLSYVKITA
                     AGAGMEGMENLALYYAALPHALILSLNEGVLTRSMDREAARKPGAAAATMPATQPVGP
                     WLGESYCLRLAPRFLQSIMDAGKNELQAQAQETAWRNIPILNEFKRLLPDQDPAQAYE
                     RVFGIALIDGAGGHYDWDEKWQTMQSSIYGSPAQPKQGPTVLDTVKGMGGNFGVTFEH
                     GGLRARAEIELPGQ"
     gene            complement(13018..13299)
                     /locus_tag="VLI90_20310"
     CDS             complement(13018..13299)
                     /locus_tag="VLI90_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16619.1"
                     /translation="MSLKSFCSFALKVIAALAASTSQYRTQLSCWLRFPERDRIAQLG
                     AEVRSFPVTRYRDYLILYRQISQGVEVLRGCIAHEICRDSSSDSAIAWC"
     gene            complement(13336..13623)
                     /locus_tag="VLI90_20315"
     CDS             complement(13336..13623)
                     /locus_tag="VLI90_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16620.1"
                     /translation="MPITITLPPELEQYVREKIASGQFTDESHIVTLALHYLKSSDDL
                     FLDSEDLRKLVAEGLDEANRGLSKPWDPDEIWAEVERRYAERESTPDKKAG"
     gene            complement(13675..>14885)
                     /locus_tag="VLI90_20320"
     CDS             complement(13675..>14885)
                     /locus_tag="VLI90_20320"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HSV16621.1"
                     /translation="FPIELSPTQHFTQPPPRYSEATLVKELEKQGIGRPSTYASIIQT
                     IQDREYVEQIDRRFHATMLGSIVTDKLIQAFPEIMDIKFTAGMELKLDEVEEDHLDWV
                     KLLQDFYGPFHAVVDGALGKIEHAGGTASPYKCLKCGKPMVYRISRNGFFLSCSGYPE
                     CDGTQPVDRQGKPTVREVSQHKCPNCGREMIKRKGRFGEFLGCSGYSVKNEKGEPSCT
                     TIINLDKEGNPQPPKIKINTTVACEKCGSPMILRDSKRGPFLGCSTFPKCRSTKMMKK
                     LEGADLKQVEALIPLLKEGAAKSAEMIAKILGANPAAAGAMRPTTIATDIDCDECGKP
                     MMIRKGRRGYFLGCSGYPKCKNTGEVPAKLVEELGLNGDGEAPKNGKAAAEASSSNGD
                     GEIETDLTVD"
BASE COUNT         2571 a         4687 c         4916 g         2711 t
ORIGIN      
        1 acgatcggcc acaccggctc gcccgcctga aacgccgcct gcgtcacgta atacatcgcg
       61 tagtacgcat agcgcgtctg atcgttcggc agcttcaccg ccaactgcgg cgtcgcccgc
      121 gttagcacgt cgttctcacc cgcattgagc agataaaggc tcagtacgcc taccgcggtc
      181 atcgactccg tcgcgttctg ccccggctgg tacgcgaagc cgttctgatc ggcgcgataa
      241 cacttgagca cgaaggccac cgcccgctgg gcgcgctcgc gcggcacatc gataccaacg
      301 tctgccgcgg ctcgcagagc cagcgcgttc catgccgaca tcgatagatc gctgtccact
      361 gaagtcggct catatcgcca accgccggcg ctattcacgt ccttcggcac atcctgcgcc
      421 gcgaggatca ccttcaccgc gcgcccaatc gccacccgta tccgttgtcg ccccgcctcc
      481 gttgtctcca cgccgagtga ctccgccagc gccagcgtga cgatcccctg cccgtacatc
      541 cggctgccgt ccactttgcc gatatatcca ttctccggca tctgccgaag ctcgaaatcg
      601 atcgcgcggc gaacggtcaa cccatatcgc cccacatccg gcgtgtgacc cgacgcgagc
      661 atcgagagga tcgacagtcc cgtcatcgcc acccgcgggc cgccgccgtc gatcgaaccg
      721 tcagcgttct gctggcgcga tagaaacgtg agaccacgat cgaccgattc atccagcttt
      781 ggtggtaagg tcgtttcctg cgcatgcaca ttcgcgctgc cgaggacaag tgccgcggtc
      841 atgagcgggt aacaccacgc tgaaatcttc atggtttagc tccgctgcta ccgctgagca
      901 cctcgaaata tgccttgagg gctgactcat atcccgccgg ttcatcgcgc ggcaacggtg
      961 cctgactgct gccaaccggc tcgggcgggc ggccccaagc cggcgattgc gacagcaccg
     1021 cagaccgaag tggctcgcct ggggtgaaca ggtttgcgat cgatggcgtc agtgacagtc
     1081 tcgcccgtgc tgcttcatga attgcagcgt cccatgcttg acccagcaat gacgacatct
     1141 cggcctgcct cgtacgagct tcgttcaagt caggcgggcg cttcgcaagc gccgccgcgg
     1201 cgatctcgct tgcggcgcgg gcttgctgca acggctcccg attgatcaag ccatgctgct
     1261 gcagctgttc gatctgttgc gtaatcgacc tctgaccttg tgcggccggc gcatcggctg
     1321 gcgccgccgc cgcccgaacg cgtgcctgct ccgctatcaa ctggtcgatc tgctttgccg
     1381 ccgtcatcac ctgctgcagg tcgcgcgctg cggatggatc ggcggagatc gaggctggca
     1441 tcgcgggctg cgtccttgcg cgtcgcagct cgtcatccag cttctgcgat tgctcgtact
     1501 gccggcgtgc cgccgcggcg ttggcggcca tcgcagcgtt caaccggtcc gcagcgtcca
     1561 gcggttcatc ggtcgccgcg gattcatctt ccccggccca tcggcgcatc agctcgcgcg
     1621 cacgctgcgc ggtcgcgcgc gccgtcggat cagcatccaa tacatcgtgc tccatcgctt
     1681 caaacgccgc gggaaacgcg gtgcgcgggt cgccgctggg ctgcgtcgtc gcgggcaacg
     1741 actgcaatgc agacaatcgc cgggccgcgt tggcggccag ctgaagatcg ttcgcgcgaa
     1801 taagatcacc gtcagcgcgc gccgcttcca cgagtgcccc ggcagccagc cggcggccca
     1861 gcggtggtgg ccagtcgttt tgacggccaa gccccgcggc gaacgcgcgc gacgccgcga
     1921 ggaagtctgg ccgatgttgg ccttgcagga aggcggatcc cgccgctgac cgctgtatca
     1981 gctgctgatc gagatctgcc gcgtgaacat ggacgcccat cggcctgagg ctatcctcga
     2041 ccgtcaattc aatttgatgt tgcaccgcgg ccagttctgg tgtgcgacca tttgcggcaa
     2101 gctcgctcaa catccgacgc tcggcaacca cggcggccgc tcgctcggca ttcgtcagcg
     2161 ccacaattga ttcgtgcaca tgagtgatcg cgtcagcagt atcggcgagt ttcaccttga
     2221 tctcggattc cggagcgttc ccgctcatgg ccccatccac taccgcagcg caagtttcca
     2281 cggtatcgac gaggtttgac aacgcgttgc tcatggattg cgaggaatcg ccgatcacgg
     2341 ctttgataag tgcgtcgcgg gcagatgacg ccgactgcag cgcatcagcc aaacgatggc
     2401 ttcgccgctc cggttgatca ctttcggctg cgaccaattc gtcgtgcaag tgcccggcga
     2461 gttccccggc ggcggcgagc gccgcgaggc gcgccggcgc ggccgggtcg gtcggttcgt
     2521 tcgtgatgag caggtgccag ggttcgctca gcgccgattg cccgaggtgg tcgcgagctt
     2581 cgacgacgac gtagatcacg tcgccgacag ccaatcggcc cagttcaatc tttgccagat
     2641 caagccgctg ctctccctgc agtcgccgag cgtcgccgga gattgggatg gtgacgtcgc
     2701 ccgatatacc gggcgccgcg gcgctgaccc gtcgcacgtg cgccatcagc acttcaacgc
     2761 cgtagtcatc ctccgcttca tagcggagcg tcatcacatc gtcggaccga acggcagcaa
     2821 tcttcgggcg cggtggcacg agccgaacat gcggcggatg atctggcatg gcgcggatcg
     2881 agccgcgcga caggccgatc ccatcaacgc cgttccgcgc ggtcatccgc agcgtgaacg
     2941 gctcgctttg tcgcacgctc agctgcgcct gttggatgga tggctcagcc gtgggattca
     3001 gcgaaagcgt tgtcgagccg atcagcatgg ctgctgcttt gagcggttga ttgccatgaa
     3061 tgctgaccat tgccgtggtg ccgacaggcg cttcgatggc gccgtccgtg ttggaaacgg
     3121 tccgcggcgg aagccgcgtg tacgctggat acgtgtagcg aatgcgaaaa tcgaccaccg
     3181 ccggtgggcg aaggacgtga acggtgtaaa tcggcgtgat cgcatcgcca gccgtgacga
     3241 agtagctgag gtcgtggtcg gcggccggaa tcgaataggc gaactcgcgc gggcgcagtt
     3301 gtgtcgccgg cgtggtggac caggactgac catcatcgga gaggtgaacg atcggcacgc
     3361 tgttggtgtc gagccgctcg gtgctggcaa gaatccgcac gggatgtcct tcgagcacgt
     3421 cgatgttggc aggcgtgacg ctcagctgaa ccgtggtcac gggcgggaca tcagccaagg
     3481 gctgggcgaa tcgcgataag agccggggca gcccgagcgg tcgtacaaag cagagcgcga
     3541 cggtgagggc gagcagcatc gtcaatgcga tgatcgcacg ccacacgggc gtgccatcaa
     3601 cgagcctgcc gccgcggtcg tttgcgacgt ctgcggcgac gccttgcgcg aggtgttgca
     3661 ggatttgcgg tgacccgcgg tggctcgtgg gctcgttgta ttgcgatgcg acggtaatga
     3721 gtcgctgccc gaggatcggg tgcagttgat cgatctcggc ggccgcggac agccaatcga
     3781 actgccgacg aaacgagatg cgaagttgcg gcagcacgag cacaacgaac gcggccacgc
     3841 cagcaagcaa cgccgccaag cgcgccgcgg cgcgcaagtt aagcgcgcga tcggcgaggc
     3901 acattagcag agcccagccg atgaacaccg tcgctgcacg cgcgatcgcc ttcgctatca
     3961 cgacgcgtcg gcggcgcgcg acgtagtcgg tgacatatat gcgaattgga ttgggcagct
     4021 catcgatcat gatcaatatc aggccaagcc aaaacgtttt cgcagtgccc attcgacccc
     4081 gaggcaaccc acgacgaacg cgtaaagctg tgggctgtcc catagcgtgc gttcgacgaa
     4141 ccccgccgcc tgcggttgaa tttcggtcaa ccgccggggc agcgtgtcga tctggtccag
     4201 ccgcaaaaat tctccgccgg atgcgcgggc cagttggcga agcagttgat catccaccgt
     4261 caggtcggcc atctccgcct gataggaccg ctgcacccgc agcggcaccg ccggatgagg
     4321 cagtgtcggt ccggcgtcga gttgcaactg atacgaccct tcgggcaaat cgaccagcgt
     4381 cgtcgtaaag cggccatccg cgctggccat cacctgcgac cgcacgatcc gcctatcccg
     4441 cagcacgctg atgaccgccc gggcaccctt cgccgggagc tcgtcttcgc ccagcacgcg
     4501 cgcccgcacg cgcacgggtg tggacggcgt cacttccagc gcgtcggcgt ccagcgccac
     4561 cggcccctcg accacgtcgt atggttcctc cgctgcatag cgcagcagtt gaagccagaa
     4621 acgctgttga tcgcgaccgc caaccttgta acgccaccgc cacgtttcgt tgatgccgag
     4681 gatgaaggat cgcccgaagc cgatgcgcga ttccgtcagc accggctgcg ccgtttcgcc
     4741 atccattaac agcgaccggg catccggctt cagcggtgag atgggcagaa accggtacat
     4801 tcccggcaat tgttgccatc ggcgatccat cggttcgttg ccgctcgcca gccgaagtgc
     4861 atccactcga ttggtgccgg gcgaggggat gacgtggacg ccagggcgat cccccgagga
     4921 gatgtgccac gccggcgctg gcccgatgat cggcaacagc gccgatgctg ccggatgctg
     4981 cagatactcc gccggcaggt gggcatcgcc agcgacgagg atcacgctgc cgccgcggtc
     5041 tttgacgagt tggctgagcg cgccccactg tgcggcgtcg agcgaatcga cggcgacatc
     5101 gaacagcatg atgacgtcct gccgcaggat gtcccgcggc gacaacggca aaggtgcgcc
     5161 gttgctcagg atgccatcgg ccaaccggca ccatggctcg cgcgacaacg cgctacatac
     5221 gtattggaaa tcccacccgg ccgacccggc gaaggcggcg atgcgaatgg gatcgctctg
     5281 aattaccttc aaccagcgct gcgcccggtt gttctcggtg gtcgcttcac ccgccagtgg
     5341 agagatggaa accgtgagca actgccctgc cgccggcgtg gccgtcacct cgtgtgtgaa
     5401 ctccaccgtt gtaacggcat cgccggcaag cgcaatctgt cgcgtctgtt tcgattcatt
     5461 tgctccgccc acttcaaacg tcacttccgc cgcggtcccg gcggggactc cggtggcgcg
     5521 gacgtcggcc cgaacggtga ttgtctctcc catgaatgtg ctgctcggcg cgctgatgcg
     5581 caggacagcc gcgtccttca gcgcctgcgg cggcgcgacg ctgacggcat agacgggaac
     5641 cccgccggag gcgagcgtgg agggcaccgt tgtgtcgcca ccgaccggcc ggccgtcgga
     5701 cagcaagacg acgccgcgga ccggcgtgcc ttgcatgtaa tcgagcacag ctcgaacggc
     5761 gccggtcaaa ttgctgacag cgccgtcggc ctcgatcgga tgcgatggtg ttgcggttgt
     5821 ctgatcgctg gcgaacggcg tcacgcgatc gccgacggcg taataaagca cgggcatcac
     5881 cgcggccaac tttgcggccg ttccaccgtc gccggtgatg gccgttatag ccaagtcgag
     5941 ccgcgattgg tgagataggt cctggcatac ctcgtgcacc gccgggtcgt cgcgaaagag
     6001 ctgttcatca gcaagcgtcc gagcgtgctc gatggcggcg acggcggatt gaacgcgcga
     6061 cggaagcgcc tcgagccagg catcgaagtc aggtcgagcc gactgcgaca gcagcgtcat
     6121 cgcctcgtgg gccacgccgg cgcttggcaa tgtcgaaagc tttcccgcag ctgaccgcgc
     6181 cagggcgcgc agttgttcca cattttcgtc taggcgcgct tgtgccgcgg cgacaccacg
     6241 gccggcgagg cgcgcgtaat ccagctcgct gcgctgccgc gcaatggcgt cggcgagcgt
     6301 ggcgatctga tccgcgtccg cctgtagcga cgccgccgtc tcgtcgcgca cgccgctagg
     6361 cagttggccc agcgctgacg cgagcgcgat cagctgtgca ggtgaccggc cggtgtcgct
     6421 cacgctcatg ctgcgggagt tgtcgaccag cacgagcagc ggccctcgct cggcatcggt
     6481 gcgcggacgg atcaccgacg gctgcagcag cgagatggcc agtgcgccca tcgccccggc
     6541 gcggagcatt ggcaagagcc atcgccagcg gcgcgacaat gaactcacct gcgatggata
     6601 caaccaccac gtcagcgcaa ccgcgccggc gaggatcgcc agtacgatcg gcaggcgctg
     6661 cggtgaatac cagtagaggg cggcgagcat ttagcgcggt tcaatcacgg tcacggttcg
     6721 ctcgaccaac gccgggcgag cagcatttcg gcggcggcga ggacgatgac gccggccatc
     6781 accggcatcc acaattcgcg gccagcgcga tcgcgcgcca ccgtcgagac gaccggcgtc
     6841 tcaccggcgt cgatgtgttg aaggccaaca gcgtcggtca cggcagccca ttgcgcatcg
     6901 ttcatcggtg tcaaatcaga ctcggcccgc ggtgagacaa tcacataatg cagcaggcgc
     6961 tccttgcctt cgctgcgata tcgcaagcga tagacgccgg gttcgtcggt tcggttgaat
     7021 cggatctcgg tgcgatcttc cagccgcaac acgggcagct gctcggtctc gccattagga
     7081 aggcgaaggt tggccgagtg ctcttcgacc ggttcagcga tcgacgcgat gatgggttgg
     7141 ctcggggcta aattgcgatt gttcggccca ttgccagcga gatatctgac ggctgattga
     7201 agaaacggca aatagaacga cgtctgcggg agcgtcgtcc aatcggcatc gagcgacgtc
     7261 gtcaccagca ggacacggcc acgaccggcg gcgccttcga tgaggaacgg atagccggat
     7321 gcatagctgc ccagcacgcg cacatcggcc gttcgcggtg tggcggggaa ataacgaagc
     7381 accgccgcag tgccgaggat atcggtgtgg ccgtttagaa agccgaagat cggatgcgtc
     7441 aaatcgacgt caacgagcaa tgttccggtg gtggcaagcg gtgtcggtgg tgacagtgac
     7501 gcgggcagga tgcccgcgcc aaagcggaac aagacgttgt tgtattcatc cacgcgactg
     7561 acattgcctg gcgcgatcag catgcccccg ccgctgaaga cgaactgttc gaggtcgcgc
     7621 gcctgggatg agccgaagcg atcgatgttg gcgaggatga cgacatcgac gccatcgaga
     7681 tcggcagcgg cgaacttcga cggcacgaca actttgacga tggcggggtc ggcgccaccg
     7741 ggtgcgccgc tgctggcgtt gggcgtgagg gccaggcgga tcagcgaggc agcgctccac
     7801 cggctgggtt gagtcttccc cggatccgca tcttcatcgc caacgatgag caccttcaac
     7861 ggcgcgtaca catcgaccgc gcgatcgagg cggttgtcgg cggtcaatcc accggctttc
     7921 agcgcggcgg tgatcaggcg cgagccgctc tcgggcgcgc ggatcgtcgc atgcaggacc
     7981 gcggtggaat cgggggcgag ggtgaccggc agctctgcca ccgcgcggcc gccgaccgac
     8041 acggtcatgg gaatgttgct gtgcggctga gcgccgatgt tccgtacgtg gatttcgaga
     8101 tcggtcggct ggtccttgat gatcggggga ttgagcacgt cgagcgattc gacgacgagg
     8161 ttattcgcgg tgtcgccgcc aacggtgatg acggtggtgc gcggcgcggg gccgccgggg
     8221 aagcgggcgt gccacgcgcg gcggaaggga tcgctggcga cgcggcgcca gctgatggcc
     8281 tggcgatctg tgatgatgta aatctctcga ttcacgcgct cgtagcgttc cagcacgtcg
     8341 gccgcgcgcg acaatgagtt gacaaagtcg gcctggccga agcctgggcg cacggcggcg
     8401 atgcgcgcgg cgacggattg aaggtcgctg gttggggcac gatccgccgg cgccagcggt
     8461 gtgccggtgg ggatgaacgc gacctggtcg ccgcggttca tggcggacag gatctggagg
     8521 gcggcctggc gcgcgaggtc tagccgcgag cggccgtttt cgacgcacgc catgctggcc
     8581 gagtcgtcca ggatgatgac ggcggtcgcg gtgccgttgc cgccgaggtt ggcattgtga
     8641 ctgctgatgg ccggtcgcgc gagcgcgagc gcgagggcgg cgacgatcag cgaacggacc
     8701 agcagcagcg accactgctt gagccgggtg ctgcggcgtt gtcgcgcatc gacgccggtc
     8761 aggaacatca tggcgcccca gtcaacggtg cggtagcgcg cgcggctcag caggtgcagc
     8821 acgaccggga cggccgcgcc accgacgcct gcgagcatga gggggttgag gaacatcttc
     8881 gtgcaccgat catcgcggcg gcgcgatcac accatcgccc ggcgatggag cattttcgtg
     8941 agcaggtcga tcatcggccg attcgtcagg aaggtgtggc actccgaccg cagcgaccgg
     9001 caggcttctt ccaactcgtc tcgatgacgg ttgaagttcg cgagataact ttgtcgcacg
     9061 agcgccggct cgcagaggta cgctggctcg tgttcgaacc cgcggaagtg cgaccaggtg
     9121 cggaacggaa attcctgctc gtcgggatcg agcacgcgca ggcagatggt ctcgtggcgc
     9181 gcatagcgca gccgggcgag cgcgctgcgg atggccggcg ccggctcgaa caggtcggac
     9241 acgatgatga ctaaaaggcg gcgatcgatg cggtcggcga cctgattgat cgccgccgac
     9301 accgcggcag gtccggcggg tgtcgcccgc tcgagcatgt cgatcacgcg cgatagctga
     9361 ctgtggccac tcgccggcgg cagccagctt tccagtgact tgcccgccac ggacaagccc
     9421 acgctttcgg tttgcgcgag catcaggtac gcgagcgatg cgaccaggcg tgcggcgtag
     9481 tcgaacttgg tcgcgcgacc gccaccggcg taggccatgg acccactgcg gtcgagcacg
     9541 atgacgcagc ggaggttggt ctcctcgtca tactccttga cgaacggtcg atccgtgcgg
     9601 gcgaggaggc gccagtccag tcgccgcggc tcatcgccgg gcacgtatgg gcggtgttgg
     9661 cggaactcga cgctcgcacc cttgaggagg cttttgtgca aaccgctggt gctgccttga
     9721 accacgctgc gcgcggtcag ctgcatgtgg ttcaagcgct cgaccagcga gggttggaga
     9781 tagcggctga cgaggctcat cgtgcatcaa gcgtacggtt gacgcggccg aatgtgaata
     9841 tgccggtgca cacggttgga gctgtaggtg taggtcttat ccgtggcacg gccgtctcgt
     9901 agcgagacgc ccgtgccacg aactgcgcca tacatctacg ttctgtcttc gattattctt
     9961 cggggggtgc ccattccacc gagaatgagc gagatgttcc aaaaagcgaa agcagaaata
    10021 tatttcggcg gtagtgactt gggcccataa gtttccgttg agatatcgca gtcgcgcgtt
    10081 gacgttgacc gccatgcgag aacgtggctc caaaccctca tgaagctcga tcactgcccc
    10141 ggcaattcaa tttccgcgcg ggcgcgcagg ccgccgtgtt cgaaggtgac gccgaaattg
    10201 ccgcccatgc ctttcactgt atcgagcact gtgggtccct gcttcggttg cgcgggcgag
    10261 ccgtagatgc tggactgcat cgtctgccat ttttcatccc agtcgtaatg cccgccggcg
    10321 ccgtcgatga gggcgatgcc gaagacacgc tcgtatgcct gcgccggatc ttgatcgggt
    10381 agcaggcgtt tgaattcgtt caggatcggg atgttccgcc aggcggtttc ctgggcctgg
    10441 gcttgaagtt catttttacc ggcatccatg atggactgca ggaaccgcgg ggcgaggcgg
    10501 aggcagtagc tttccccgag ccacgggccg accggctggg tggcgggcat cgtcgccgcc
    10561 gccgcaccgg gcttgcgggc ggcttcgcga tccatcgagc gcgtcagcac gccttcgttg
    10621 aggctgagga tcagcgcgtg cggcagcgcg gcgtaataga gcgcgaggtt ctccatccct
    10681 tccatgcccg cgccggcggc ggtgatcttg acgtacgaaa gatcgccgtg cttcagcgtt
    10741 tcccacgtcg tcaggttcgg ggcggattga tcggccaacc cgtgcagcgc cgtcaggaac
    10801 gcggcgagct tcagcgggct gctcgattcc acgtacagcg caaccggtag tcgcgccact
    10861 tgatggcgga tgaattcatc ggcctgatcg gccttggcca gctcgtccca gaagccgtcg
    10921 tcgtcgccat agagcgacgc cgaatgaccg agccaagcga gtggatcgat cccggcggcc
    10981 ccaccgccga aactttgggc gatgttggag aggttgatca actgtttgga cttggggttg
    11041 aacgccatcg cgatctgggc catcgcgtaa tgcggatcgg cggaatcggc ggtgagctta
    11101 gcgccggcgg aaagctcgac cacgtcctgg taatcgctgc tgaccatgag cggcatcacc
    11161 gtggtgtcta cgccgattga agtcgcgccg agactgatac gaatggcgat cggatcgaac
    11221 cagcggaagt tgcgttcata gccgctgcgc cagcgatcat acgcgtcggc ctcttcgcgc
    11281 gtgacggtgt cgagattgag ttccgcgatg ggcgtcatga aatcgagcga gccgaacgtg
    11341 tcggagaaaa cgccgccggt gtggagcgac atgcggtcga ggcccgggac gaagcgattg
    11401 ggcgtgacga tttgtcgctt tatcggcccc atcgcgatgt cgcgcgcgta ctccgcctga
    11461 agatcgctca tcaccgcggc ggcgcgcgtg cggcgggagt caccgatgcg ccagcgcggc
    11521 gaacaccatc ggcggatcgt ctggtcgctc agcacgagca gtgccgtctc cttctcatct
    11581 ccaaggcgat atcgatcgcg gaagaaggtg tattccggcg tcgaagatag cgccgccgcc
    11641 ttcgcctgct gaacggatgc gagcttttcc agctggacca gcgagttggt gacgacgacg
    11701 gcggggccga tcgtggcgag atagctgcag acgccgcggt caccgctcac aaggccgctg
    11761 tacgagatgc cggcgacttc gccgttgacg ggcgatgcct cgggttcgct cgcacgccgc
    11821 atcatctgct gggcggcgat gagccggttc aaagttgccg cgtcttttgc ctggaagatc
    11881 acggcgaggt ctgagccgac gcgccagtac ggatccgacc cggtgatggc gacgctgtcg
    11941 atcagcgacg gcccgagcag ccggcccagt tgtgtcatcg acaggcacag ttgccgctcg
    12001 tagcgctgcc gcgtccgcgc gtcacccgcg gcggactcaa tcgtggagat gaacgggctg
    12061 agttgctgat ccgcgcggtc cgccagcgcc atgcacgcgg cgaacgacgg gaagaagatg
    12121 gcatgttgat cggcggggat gatggccgcg agggcgtcgg gctgcgggtg caggtctttg
    12181 accagcggct tccagtcgaa cggctgcgtg gcgacgccct ggaggtccgc cgttttcacg
    12241 tcgaccgccg cgggttgcgt ggtggagggc agcgcccgat cgagctgcag gttttcgctg
    12301 accgcccggc caccgctggc gaacccgaag gtctggtcca gctcatcgtc gtggttcgat
    12361 tgtgcgagct gctcgggcaa ttcgggcgga gcatcttttc caagttcttg gcgcgccgcc
    12421 cgcacgcgct gccggaagta cgctgcgccg ggcaggctgc tttgctgcag gtgctggtaa
    12481 tacgcgaatc gagccttgag aaaatcctgc cgcgtgccgg ggtggtcggc gctgttgacg
    12541 gtgaagcgca cggcgttaac ggcggtgttg tcgtcgttcg cgccatagat cgtgccggca
    12601 agctgccccg cggcatcgct gcgaatcacg atccgccgcg gggccaagtc ggccagccgc
    12661 tgcgtgatcc aaacgccgtc gtcaccggca aaatacgctt cggcgccgtt gtccataacg
    12721 atgcgggcgt cgatcgagcc gttgcggttg gcgacactcg ggccggatgt gggcgcatga
    12781 tcgacgccgc cgttggggaa cgtcagctcc ttcagcggga cattggtgta tacctccgca
    12841 tgtacgaccg cggcggcgat gagcgagaga acgggaacca gcaacgcgac cagcggcgtg
    12901 gagaggcgac gtgtcatgat gaaccccgcg cggttaagtt aggaaccaca aacccgcgga
    12961 gcatcctaac gcgcggcgtt gcagccatgc aaatttccga tatcggattc ggaaatgtca
    13021 gcaccaggcg atggcggaat cacttgaaga atcgcggcag atttcgtgcg ctatgcagcc
    13081 gcgcagcact tcaactcctt gggagatctg gcggtaaagg atgagataat cgcggtagcg
    13141 ggtaactggg aagctccgca cttccgcgcc tagctgggcg atgcggtccc gctccgggaa
    13201 gcgcagccag caacttagtt gtgtcctgta ttgcgaggtg gacgcggcga gcgccgcgat
    13261 gacttttagt gcgaagctgc agaaggattt caacgacatc ctgccgcgcc agcggggtga
    13321 ggatgacggc cgccattatc cggccttttt atcgggagtg gactcgcgtt cagcataacg
    13381 ccgttcaacc tcggcccaaa tttcgtccgg atcccagggt ttgctcagcc cccgattggc
    13441 ttcatccaac ccctcggcaa ccagcttccg cagatcttcg ctgtcgagaa acaggtcgtc
    13501 ggacgacttc aagtaatgca acgccagcgt gacgatgtgt gactcgtcgg tgaactgccc
    13561 cgacgcgatc ttctcgcgga cgtactgctc cagctctggc ggaagtgtga tcgtgatggg
    13621 catgggcgct cccgcgttgg ctgcccaaat tataacccac gccgccgcag cggatcaatc
    13681 gacggtcagg tccgtctcga tctcaccgtc gccgttggag gatgacgcct ccgctgccgc
    13741 cttgccgttc ttcggcgctt cgccgtcgcc gttcaagccc agctcttcga ccagcttcgc
    13801 cggaacctcg ccggtgttct tgcacttggg atacccgctg cagcccagga aatacccgcg
    13861 gcggcctttg cggatcatca tcggtttgcc gcactcgtcg cagtcgatgt cggtggcgat
    13921 ggtggtcggc cgcatcgcgc cggcggcggc ggggttggcg cccaggatct tggcgatcat
    13981 ctccgcactc ttggcggcgc cttctttcag cagcgggatc agcgcttcga cctgcttcag
    14041 gtcagcgcct tccagcttct tcatcatttt cgtcgaccgg catttgggga acgtcgagca
    14101 gccgaggaat gggccgcgct tgctgtcgcg caggatcatc gggctgccgc acttctcgca
    14161 ggcgacggtg gtgttgatct tgatcttcgg cggctgcggg ttgccttcct tgtccaggtt
    14221 gatgatcgtc gtgcagctcg gttcgccctt ctcgttcttg acggagtagc ccgagcaacc
    14281 gaggaactca ccgaagcggc ccttgcgctt gatcatctcg cgcccgcagt tcgggcactt
    14341 gtgctggctg acttcgcgca ccgtcggctt gccctggcgg tcgaccggtt gggtgccgtc
    14401 gcattcgggg taaccgctgc agctcaggaa gaaaccgttg cggctgatgc gataaaccat
    14461 cggcttgccg cacttcaggc atttataggg gctggcggtg ccaccggcgt gttcgatctt
    14521 gccgagcgcg ccgtccacca ccgcgtggaa cggcccgtag aaatcctgca gcagcttgac
    14581 ccagtcgaga tgatcttcct cgacctcgtc cagcttcagt tccatcccgg ccgtgaactt
    14641 gatgtccatg atctcgggga acgcctggat caatttgtca gtgacgatcg agccaagcat
    14701 cgtcgcgtgg aaccggcggt cgatctgctc gacgtactcg cggtcctgaa tcgtctgaat
    14761 gatgctcgcg tacgtgctgg gccggccgat gccctgcttc tccagttcct tcaccagcgt
    14821 cgcctcgctg taccgcggcg ggggctgggt gaagtgctgc gtcggcgaca gctcgatcgg
    14881 gaaaa
//
LOCUS       DATIMH010001179         1803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4029034, whole genome shotgun sequence.
ACCESSION   DATIMH010001179 DATIMH010000000
VERSION     DATIMH010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4029034"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1090
                     /locus_tag="VLI90_20325"
     CDS             146..1090
                     /locus_tag="VLI90_20325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16622.1"
                     /translation="MPITLVLGLIILAACEVLLFIDVHQTHRGRLTTELQIDQILEQP
                     TSGYIAGLARFVAVNMTALAWIGYLVFLDGILTWQTGASPVRRRPHHFAMLCLASIFI
                     WCVFDFINFGRGINAWVYIGLPGRLREKFLGYFFAFATIVPGMLMSGQAMLNARWFDW
                     ARSREWRMPRWGKLIALLAGIGMFIWPLYYPNPVTNLTLWTSFVFLLDPINLKLGRPS
                     MFRDWQNGWYGRTLAAFAGGLLCGLLWEFWNYWALAKWTYHLPFLGRWENYRYFEMPL
                     PGLIGFVPFGIECWVMWQTIRIAFDGLVEPLTSERELL"
     gene            1087..1542
                     /locus_tag="VLI90_20330"
     CDS             1087..1542
                     /locus_tag="VLI90_20330"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSV16623.1"
                     /translation="MISERLQALSITLPKVAGPFGAYVPVKRVGNLVYVAGQLPMKDG
                     KLLATGQVPSRASIDEAKQAARQCVINALGAIQAVEGSIDNLIGVVRVGAFVSSDTSF
                     TQQPQVANGASELLLELFGDAGKHVRAAVGVNTLPLDASVEVEFIFQCS"
BASE COUNT          319 a          540 c          572 g          372 t
ORIGIN      
        1 ccgcaggggc acatgtttct tccttcgaac gaaaaacatg tgccccttcg gggctaccgc
       61 cacgcggcgg atcaatcgca cgcatcgcgc aatgagcacc gtatcgtcgc cgtccctcgc
      121 caaccgtctg aacactcgca tcaacgtgcc catcacgctc gtgctcggcc tgatcatcct
      181 ggccgcgtgc gaggtgctgc tgttcatcga cgtccaccaa acccaccgcg gccggctgac
      241 gaccgaactc cagatcgatc agatcctgga gcagccgacc agtggataca tcgccgggct
      301 ggcgcggttc gtcgcggtga acatgacggc gctggcgtgg atcggttacc tcgtcttcct
      361 cgacggcatt ctcacgtggc agaccggcgc gagccccgtg cggcggcggc cgcaccattt
      421 tgcgatgctt tgcctcgcgt cgatcttcat ctggtgcgtg ttcgacttca tcaacttcgg
      481 ccgcgggatc aacgcgtggg tgtacatcgg cctgcccggg cggctgcgcg aaaagttcct
      541 cgggtacttc ttcgccttcg cgacgatcgt gcctgggatg ctgatgagcg ggcaggcgat
      601 gctgaacgcg cggtggtttg actgggcgcg ctcgcgcgaa tggcgaatgc cgcggtgggg
      661 gaagttgatc gcgctgctcg ccggcatcgg gatgttcatc tggccgctct attaccccaa
      721 tccagtgacg aacctgacgc tgtggacgag tttcgtgttt ctattagatc caatcaatct
      781 gaaactcggc cggccgagca tgtttcggga ttggcagaac ggctggtacg gccgcacgct
      841 cgccgccttc gccggtggat tgctgtgcgg gctgctgtgg gaattctgga actattgggc
      901 gctggcgaaa tggacgtatc atctgccgtt ccttggccgg tgggagaact accgatactt
      961 cgagatgccg ctgccgggcc tgatcggctt cgtccccttc ggcattgaat gctgggtcat
     1021 gtggcagacg atccggatcg cattcgacgg cttagtcgaa ccactgacca gcgaacggga
     1081 acttttatga tttcggaacg actccaggca ctgtcaatca cgttgccgaa ggtggccggt
     1141 cccttcggcg catatgtgcc ggtcaaacgc gtcggcaatc ttgtctatgt cgccgggcaa
     1201 ctgccgatga aagacggcaa gctgctggcg accggccagg tgccgtcgcg ggcatcgatc
     1261 gacgaggcga aacaagcggc gcgccagtgc gtgattaatg cgcttggcgc gatccaggcg
     1321 gtcgaaggtt cgatcgacaa tttgattggc gtcgttcgcg ttggcgcgtt cgtcagcagc
     1381 gacacgagct tcacacagca gccgcaggtt gcgaatggcg ccagcgagct attgctggaa
     1441 ctgtttggcg acgccggcaa gcacgtgcgt gcggccgtcg gcgtgaacac gctgccgctg
     1501 gatgcgagcg ttgaggtgga gttcatcttc caatgctcgt agctgcgacg cctgcgtcgc
     1561 ctccgtcgga acacgagtgt taccgagaaa aggcgaccca ggcgtcgcgg ctacagggct
     1621 gctatcattc taccagaccg atgattcgac cctcgaccat tcacgacgtg cctcggattg
     1681 ccgaggtgat caacagccac gccgaactgg ggaagatgct tttcaagagc tacgcggagc
     1741 tgtacgagaa cctgcgcgac ttcgcggtgt atgagctgga cggccagatc gtcggctgct
     1801 gcg
//
LOCUS       DATIMH010001180         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4902731, whole genome shotgun sequence.
ACCESSION   DATIMH010001180 DATIMH010000000
VERSION     DATIMH010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4902731"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2061
                     /locus_tag="VLI90_20335"
     CDS             <1..2061
                     /locus_tag="VLI90_20335"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HSV16624.1"
                     /translation="ARRDAIHRALLTGLLSNVGNKMEKADYAGPRGAKFYLFPGSALF
                     ERKPAWVMAAELVETSKLYARTVAKVSPEWIERAAAHLIKKTYTDPVWNPQRADVIAG
                     EKVTLRGLVIVPHRTVSYGPIDPRTAREVFVHHALVEGEFRTQGEFFKHNQSLTRQVR
                     LLEAKARRSDLLAEPQQRFAFYHARVPGDVYNGMLFERWRKRAEKEDPRVLFMSIDDV
                     ARPEAKGIDAATYPDQIEISNLRFPLSYVYDTGRADDGITATIPLALLNQVPAGPFEW
                     LVPGMLGEKIVALIRSLPKGVRTRLVPAPEFAAKAQAAIGGGSPSAPFLDVVAHQLGK
                     FIDEVIPTQAFDLKDLPHYLRMNFRIVDEAGKTVAMGRDLQAIRARLGIKARESFAAL
                     PPPEFTRDHLTSWDFGDLPERIEIHRGGATFSGFPALVDLGNSVALRVLDSREASKQA
                     NRGGVRRLFMIQLDQEMKQLARTFTGVEQMSLHYKPLGKWPELKDQLLSAAVDRALYR
                     DVDEIRTRDIFIAVANDGWRRLTIAARELNAVAQEILARYHEIALALARDFPPMLLAP
                     VQEMRDQLAQLMTKQFLQSTPPQWLEHFPRYLRAMSIRLSKLLNAGLKRDEEISWMIR
                     PLWRAYIERRAAHAARGVVDPQLWQVRWMIEELRVSLYAQELKTSMPVSVQRLERQLS
                     LVAA"
     gene            2315..>3146
                     /locus_tag="VLI90_20340"
     CDS             2315..>3146
                     /locus_tag="VLI90_20340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16625.1"
                     /translation="MSSRRDRSSQTLEVAVIPVIETLENRQLLSVALPGAVASTKLTG
                     TIIGTPGSYNNTGNTINKAFDSGFTTYFDAQAADGDWAGLDLGGPTQITTVKFAPRSS
                     WAGRMVGGVFEGSNTADFSSGVVNLFTISAAPVEGSFTSQTITDTGSYRYVRYLGPTG
                     SHGNVAELEFDGVVPAPAPASSALSGTIIGTPGSYNNLGNTIVKAFDGNLSSYFDAQQ
                     SDGAWAGLDLGAPAQITQIRFAPRSSWASRMVGGIFQGSNTADFSSGVVNLFTISAAP
                     V"
BASE COUNT          567 a          877 c         1097 g          605 t
ORIGIN      
        1 gctcggcggg atgcgattca tcgcgcgctg ttgacggggt tgttgtccaa cgttggaaac
       61 aagatggaga aggcggacta cgcggggccg cgcggggcga agttttattt gtttccaggg
      121 tcggcgttgt tcgagcggaa gccggcgtgg gtgatggcgg cggagttggt ggagacttcg
      181 aagttgtatg cgcggacggt ggcgaaggtg tcgccggagt ggatcgagcg ggcggcggcg
      241 catctcatta agaagacata cacggacccg gtgtggaacc cgcagcgggc ggatgtgatt
      301 gcgggggaaa aggtgacgct gcgcgggctg gtgatcgtgc cgcaccgcac ggtgtcgtat
      361 ggaccgatcg acccgcggac ggctcgcgag gtgttcgtcc atcacgcgct ggtggagggc
      421 gagttccgca cgcagggtga attctttaaa cacaaccaat cgctgacgcg gcaggtgcgg
      481 ctgttggaag cgaaggcccg gcgcagcgat ctactggctg agccgcagca gcggttcgcg
      541 ttctaccacg cgcgcgtgcc gggggatgtt tacaacggga tgttgtttga gcgatggcgg
      601 aagcgcgcgg agaaggaaga cccgcgggtg ctgttcatgt cgatcgacga tgtggcgcgg
      661 ccggaggcga agggaatcga cgcggcgacg tatccggatc agattgaaat ctcgaattta
      721 agatttccgc tttcgtacgt gtacgacacc gggcgcgctg atgatggcat caccgcgacg
      781 attccgctgg cgctgctgaa ccaggtgccg gcggggccgt ttgagtggct cgtgccgggg
      841 atgctggggg agaagatcgt ggcgctgatt cggtcgctgc cgaagggggt tcgcacgcgg
      901 ctggtgccgg cgccggaatt tgccgcgaag gcgcaagctg cgatcggggg cggttctcct
      961 tccgcgccgt ttttggatgt cgtggcgcat cagctgggga agttcatcga cgaggtgatt
     1021 ccgacgcagg cgttcgatct gaaggatctg ccgcactact tgcgcatgaa ttttcgcatc
     1081 gtcgatgagg cggggaagac ggtggcgatg gggcgcgatc tgcaggcgat ccgcgcgcgg
     1141 ctcggcatca aggcgcgcga aagttttgcc gcgctcccgc cgccggagtt cacgcgcgat
     1201 catctcacca gctgggattt cggcgatctg cctgagcgaa tcgagatcca ccgcggcggc
     1261 gcgacgttct ccggatttcc agcgctggtc gatctcggca actccgtcgc gctgcgcgtg
     1321 ctcgacagcc gcgaagcgtc gaagcaagcc aaccgcggcg gggtgcggcg gttgttcatg
     1381 atccagctcg accaggagat gaagcaactg gcgcgcacgt tcaccggcgt cgagcagatg
     1441 tccctgcatt acaaaccgct cggcaagtgg ccggagttga aggaccagtt gctgtcggcc
     1501 gccgtcgatc gcgcgctgta tcgcgacgtg gatgagatcc gcacgcgcga tattttcatc
     1561 gccgtcgcca acgacggctg gcgacgattg acgatcgcgg cgcgcgagct gaacgcggtg
     1621 gcgcaggaaa tcctggcgcg atatcacgag atcgcgctgg cgctggcgcg cgacttcccg
     1681 ccgatgctgc tcgcgccggt gcaggagatg cgcgatcagc tggcgcagct gatgaccaag
     1741 caattcttgc agtccacccc accgcagtgg ttagaacatt tcccacgtta cctgcgggcg
     1801 atgtcaattc ggctgtcaaa actgttgaac gccgggttaa agagggatga ggaaataagt
     1861 tggatgatcc gcccgctctg gcgagcgtat attgaacgtc gcgccgcgca cgcagcccgc
     1921 ggggttgttg acccgcagtt gtggcaggtg cgatggatga tcgaggaatt acgcgtttcc
     1981 ctttacgcac aagagcttaa gacgtcgatg cctgtttccg tgcagcgact ggagaggcag
     2041 ttgagcctgg tggcagcgtg aagatttgaa aaatttcctc tcataggggg cgtgcaatgg
     2101 tgtcagttag cgtggcgtcg tgtatacgtg tgcgttcttc gcggcagtcg tgtagtggga
     2161 tttgaggtcg gttgccgtcg attcaatttc tatttgccgc ctccgaatcg gtgaggtaac
     2221 agcagccagc tacgccgttt aaggcgaggc tggcggtgtt tcaatcggac gcggtgtggt
     2281 tttaacgcca ggagattttg tggtcaggaa gacgatgtcg tcgcgtcgcg atcgttcgag
     2341 ccagacgttg gaagtggcgg tgattccggt tatcgagacg ctggaaaacc gccagctgct
     2401 gtcggtggcc ttgccggggg cggtggcgag cacaaagctg accggcacca tcatcggcac
     2461 acccgggtcg tacaacaaca ccggcaacac gatcaacaag gcgttcgaca gcggcttcac
     2521 gacctacttc gacgcgcaag cggccgacgg ggattgggcg gggctggacc tgggcgggcc
     2581 gacgcagatc acgacggtga agttcgcgcc gcgcagcagc tgggccgggc gaatggtggg
     2641 tggcgtcttc gagggctcca acaccgccga cttctccagc ggcgtcgtca acctgttcac
     2701 gatcagcgcg gcacccgttg agggatcgtt cacctcgcag acgatcaccg acaccggctc
     2761 gtatcgctac gtgcgatacc tcggcccgac cggctcgcat ggcaacgttg ccgagctgga
     2821 attcgacggc gtggttcccg cgccggcgcc ggccagctcg gcgctgtccg gcacgatcat
     2881 cggcacgccc gggtcttaca acaatcttgg caacacgatc gtcaaagcgt tcgacggcaa
     2941 cctgtcgagc tacttcgatg cacaacaatc cgatggcgcc tgggcaggct tggacctggg
     3001 cgcgccggcg cagatcacgc agattcgctt cgcgccgcgc agcagctggg ccagccgaat
     3061 ggtcggcggc atctttcaag gatccaacac ggctgacttc tccagcggtg tcgtcaacct
     3121 gttcacgatc agcgcggcac ccgttg
//
LOCUS       DATIMH010001181         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1728720, whole genome shotgun sequence.
ACCESSION   DATIMH010001181 DATIMH010000000
VERSION     DATIMH010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728720"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VLI90_20345"
     CDS             <1..512
                     /locus_tag="VLI90_20345"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HSV16626.1"
                     /translation="ASVGAGRGVIYTAKSFGRYRIVFDIRHVSGNPDHQACVLVFCTA
                     PTPGVKPPDALGGIQFQVPTGGHWDYRKGHNNGGNDEFTKLDHPKFKANEWSRCEILV
                     DPATGTARMAVAQPVGSKAVEALDFKDPTAGQKGPFALQMHNRGLFDEYANIAIEEEP
                     VSDELITTK"
     gene            complement(537..>2012)
                     /locus_tag="VLI90_20350"
     CDS             complement(537..>2012)
                     /locus_tag="VLI90_20350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSV16627.1"
                     /translation="LSLAFLVSPVAMALAIAASFLLMRMIAGSFAAVVGMLLVASSPM
                     TLGLTNNPNSHATAICLVAWGMYLLLRWWQSNGLTRAIGAGLLLGAAVTVRYTEGLML
                     LPLVAVALMNLRWRNRRSWIETIALGASWALPVIILATYNWFTLHHLTGYDPTNESTG
                     FTWEYFQDNWETMLRQLYNTGLFFVLPFTVMGWVWMWRWNWRVALVLCAWTVPSLLLY
                     TAYYWAPDGAWIGYLRFFLTILPGLALAAVWTLKKLADLAAAHDGSNIVPKLAIAVLV
                     AIACGVNMNTALGEIEVEARNNLGVSIGAVRILQACPANSVIFAPDRLLNYLQLIGSY
                     QLYDTQQFNRPFIQRLANIDPDQPNGLQPQRAKEMYGRLKDLTEPEIIKEQNKLMDAA
                     LARGERVFMIFPRPQMPAAMRFLPKRLYQTQPVATWEDLENARPMRRQHFGFNRQPLS
                     PPDAVRAMTWQILEVMAAPPPPPPKARAMPTPRNATTRPVH"
BASE COUNT          402 a          672 c          598 g          340 t
ORIGIN      
        1 tcgccagcgt cggcgccggt cgcggcgtga tctacaccgc caaatccttc ggccgatatc
       61 gaatcgtctt tgacatccgc cacgtatcgg gcaaccccga ccaccaagcc tgcgtgcttg
      121 tcttctgcac cgcgccgacg cccggcgtga aaccgcccga cgccctcggc ggcatccagt
      181 ttcaagtccc caccggcggg cattgggact atcgcaaggg ccacaacaac ggcgggaacg
      241 acgagttcac gaagttggat caccccaagt tcaaggccaa cgaatggagc cgctgcgaga
      301 tcctggtaga ccccgccacc ggcaccgccc gcatggcggt cgcccagccg gtgggttcga
      361 aggcggtgga agcgctggat ttcaaggatc caaccgccgg gcaaaaggga ccattcgccc
      421 tgcagatgca caaccgcggg ctgtttgacg aatacgccaa tattgcgatt gaagaagaac
      481 ccgtgagtga tgaattgatc acaacgaagt gaagattgct gaggacgccg cctgcgctaa
      541 tgcaccggcc gcgtcgtcgc atttcgcggc gtcggcatcg cccgcgcctt tggcgggggc
      601 ggtggtggcg ccgccatcac ttccaggatc tgccacgtca tcgccctcac cgcgtccggt
      661 ggcgacagcg gttggcggtt gaacccgaaa tgctgccgcc gcatcggccg ggcgttttcc
      721 aagtcttccc acgtcgcaac aggctgcgtt tgatacaatc gctttggaag gaaccgcatg
      781 gccgccggca tctgcgggcg cgggaagatc atgaagaccc gctcgccgcg cgccagcgcc
      841 gcgtccatca gcttgttctg ctccttgatg atctccggct cggtcaggtc cttcagccga
      901 ccgtacatct ccttcgcccg ctgcggctgc agcccgttgg gctggtcggg atcaatattc
      961 gccagccgct gaatgaacgg ccgattgaac tgctgcgtgt cgtacaactg atagctgccg
     1021 atcaactgca gataattgag caaccgatcc ggcgcgaaga tgacggaatt tgccgggcac
     1081 gcctgaagga ttcgaacagc gccgatggag acgccgaggt tgtttcgcgc ttcgacttcg
     1141 atttcgccca gcgccgtgtt catgttgacg ccgcaggcga tggcgacgag gactgcgatg
     1201 gcgagcttcg gcacgatgtt cgatccatcg tgcgccgcgg ccaggtcggc cagctttttc
     1261 agcgtccaca ccgccgccag cgccagcccc ggcaggatcg tcaggaagaa ccgcaggtaa
     1321 ccgatccacg ccccgtccgg cgcccagtag tacgcggtgt aaagcagcag gctgggcacc
     1381 gtccacgcgc acagcaccag cgcgacgcgc caattccacc gccacatcca cacccatccc
     1441 atcacggtga acggcagcac gaaaaacagc cccgtgttgt acagctggcg gagcatcgtc
     1501 tcccagttgt cctggaaata ttcccacgtg aagccggtgc tttcgttcgt cgggtcatac
     1561 cccgtgaggt gatgcagcgt gaaccagttg tacgtcgcga ggatgatcac cggcaacgcc
     1621 cacgaggcgc cgagcgcgat cgtttcgatc cacgatcgcc gattgcgcca gcgcaggttc
     1681 atcagcgcca ccgcgaccag cggcagcagc attaaacctt cggtgtaacg caccgtgacg
     1741 gctgctccga gcagcagtcc agcgccgatc gcgcgagtca gtccattcga ctgccaccag
     1801 cgcagcagca ggtacatccc ccaggcgacg aggcatatag ccgtcgcgtg gctgttggga
     1861 ttgttcgtca accccagcgt catcgggctc gaagcaacca gcagcatgcc gacgaccgcc
     1921 gcgaacgaac cggcgatcat ccgcatcagc agaaacgacg cggcgatcgc cagcgccatc
     1981 gcgaccggac tcacgagaaa tgccagcgat aa
//
LOCUS       DATIMH010001182         4361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4903085, whole genome shotgun sequence.
ACCESSION   DATIMH010001182 DATIMH010000000
VERSION     DATIMH010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4903085"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VLI90_20355"
     CDS             <1..1131
                     /locus_tag="VLI90_20355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV16628.1"
                     /translation="LFYEPVGLSGETTQAVQVLQWDEPAFFTAGLTWNNMGAAGDGAT
                     WDNVTNVNWNNGSGAVNFMAADNVTFNDANNGHYAVTLNSAVSPGAVTFDNSAGNYTV
                     GGTGGIAGAGTLTKLGSGTLTISTVNTYSGDTIVSAGTLTLAAAGSIASGKVTAASGA
                     TLNVLGSLASTAAITSSGTVNFAGNSSTTTSGARTIGALTINAGTVTIQSSMLPGKPM
                     VLTTASLSGAGTLDLTNNELITTAAAAAIRGRIIAHQIISSVGAVGYLDLGNGKIEAR
                     YTLIGDADLNGAVDVGDLGALATNYGITTGGSWSGGDFNYDNMVDVGDLGALATNYGN
                     ALAGNAAATVTAVVSTSVPEPPVVALGVLARLCCLSLRRKRS"
     gene            1252..2412
                     /locus_tag="VLI90_20360"
     CDS             1252..2412
                     /locus_tag="VLI90_20360"
                     /inference="COORDINATES: protein motif:HMM:NF021534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HSV16629.1"
                     /translation="MASAQDDRAYWVATLDRIARPVLTALAARELKATMPVETRPGGN
                     KADRKSYTHLEALGRTVAGISPWIELGAGDGDEGKRRAELADLARQSIDAGTDPGSPD
                     YMNFTHGGQPVVDAAFLAHALLRAPNQLWAKLDPRVQRNVVAALAATRAIKPGMNNWL
                     LFSAMVEAALCSVGEEWKTEPVDFAIKKHMEWYKGDGLYGDGPTFHWDYYNSFVIHPM
                     LIDVLEAVSKRRDDRAALRKPILARARRYAAIQERLISPEGTFPAIGRSLAYRFGVFQ
                     LLGQMALRRELPAAVSPCQVRCAMTAVIRRMIEAPGTFDEHGFLTIGVCGHQAGMGEG
                     YISTGSLYLCTTGLLPLGLPVGDEFWTCEASDWTQKRVWAGADVGTDHAIEE"
     gene            complement(2600..3487)
                     /locus_tag="VLI90_20365"
     CDS             complement(2600..3487)
                     /locus_tag="VLI90_20365"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSV16630.1"
                     /translation="MQTPQRLAFIGLGIMGNPMAAHLLRAGHAVTVHNRTRSKADELL
                     KAGATWSDTPAAAAAHADVVFICVTDTPDVEAVLFANHGVFETIRPHTIVIDHSTISP
                     TATKRFAQRLAEHQATLLDAPVSGGDVGARNATLAIMVGGDRPAFDRALPLLQHLGKT
                     ITHCGPAGSGQLTKLVNQILVSVTNLAVCEALTLATTGNLDGKQTIEAVAAGAAGSWQ
                     LSNLGPRMITGDRKPGFMIDLQLKDLRLVQEAAADLGLHLPAVELVTSLFKQAQALGH
                     GREGTQGLFDEVRRAAEHS"
     gene            complement(3639..>4361)
                     /locus_tag="VLI90_20370"
     CDS             complement(3639..>4361)
                     /locus_tag="VLI90_20370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSV16631.1"
                     /translation="EALDICIQMADALAHAHRRGLIHRDVKPKNIILTPQGVAKLTDL
                     GLARATDDKHAAESEAGKAYGTPYYISPEQIRGDVDIDFRADVYSLGATMYHLVTGRP
                     PFEGDTPSAVMHKHLRQPLVPPDHINTALSSGVGEIIEVAMAKNRDERYGSTEDMLED
                     LRAVADGNPPQHAKRAVDLEALASIEETGKTVDLNHPSDGVGSTFSQLMENPYAGMLL
                     AAVGVSLLINLILIVVMVLTKK"
BASE COUNT          735 a         1192 c         1625 g          809 t
ORIGIN      
        1 ctgttctacg agccggtggg cctgagcggc gagacgacgc aggcggtgca ggtgctgcag
       61 tgggacgagc cggcgttctt caccgcgggg ttgacgtgga acaatatggg cgctgccggg
      121 gatggcgcga cgtgggacaa cgtgacgaac gtcaactgga acaacggcag cggcgcggtg
      181 aacttcatgg ccgccgacaa cgtgacgttc aacgacgcca acaacgggca ttacgcggtg
      241 acgctgaaca gcgcggtctc gcccggcgcg gtcacgtttg ataatagcgc cggcaactac
      301 acggttggcg gcacgggcgg gattgcgggc gcgggcacgt tgacgaagtt gggcagcggc
      361 acgctgacga tctcgacggt caacacctac agcggcgata cgatcgtcag cgccggcacg
      421 ctcacgctgg cggcggctgg atcgatcgcc agcgggaagg tgaccgcggc gtcgggagcg
      481 acgttgaacg tgctcgggtc gctcgcttcg acggcggcga ttacttcaag cggcaccgtc
      541 aacttcgccg gtaattccag cacgaccact agcggtgccc gcacgatcgg cgcgctcacg
      601 atcaatgccg ggaccgtcac gattcaaagc tcgatgttgc cgggcaaacc gatggtgctc
      661 acgaccgcga gcctaagtgg cgccggcacg ctggatctaa ccaacaacga gttgatcacc
      721 accgccgcgg cggcggcgat ccgcgggcgc atcatcgcgc accagatcat ctcgagcgtg
      781 ggcgcggtgg gttacctcga tctgggcaac ggcaagatcg aggcgcgcta cacgctcatc
      841 ggcgacgcgg atctgaacgg ggccgtggac gttggcgatc tgggcgcgct ggccacgaac
      901 tacggcatca ccaccggcgg ctcgtggagc ggcggggact tcaattacga caacatggtg
      961 gatgtcggcg accttggcgc gctggccacc aactacggga acgcgctggc tgggaacgcc
     1021 gccgcgactg taaccgcggt ggtgagcacc agcgttccgg agccaccggt ggttgcgctg
     1081 ggggtgttgg cgaggctgtg ctgtctttcg ctgcgccgca aaagatcttg atgccacgcc
     1141 ggggtcggag gtactccgac gacccggatg cttctcacgc gccgacgaat ccgggtcgtc
     1201 ggagtacctc cgaacgcggc atggggggtt gtgtcgcgtt agaatcatca catggcttca
     1261 gcacaggacg atcgcgcgta ttgggttgcg acgctggatc gaatcgctcg gccggtgttg
     1321 acggcgctgg cggctcgaga gttgaaggcg acgatgccgg tggagactcg gccgggtggg
     1381 aataaggcgg atcggaagtc gtacacgcat ctcgaggcgc tggggcggac ggtcgcgggg
     1441 atttcgccgt ggattgaatt gggtgcgggc gacggcgatg aaggcaagcg tcgcgcggag
     1501 ttggcggatt tggcgcggca gtcgatcgac gcggggacgg acccggggtc gccggactac
     1561 atgaacttca cgcacggcgg gcagccggtc gttgacgcgg cgtttctggc gcatgcgttg
     1621 ttgcgggcgc cgaaccagct gtgggcgaag ttggacccgc gggtgcagcg gaacgtggtg
     1681 gccgccctgg cggcgacgcg ggcgatcaag ccggggatga acaactggct gctgttcagc
     1741 gcgatggtcg aagcggcgct gtgcagcgtt ggcgaagagt ggaagaccga gccggttgat
     1801 ttcgcgatca agaagcacat ggaatggtac aagggcgatg gcctgtacgg cgacggcccg
     1861 acgtttcatt gggattacta caatagcttt gtaatccacc cgatgctgat cgacgtgctg
     1921 gaggcggtgt cgaagcggcg ggatgatcgg gcggcgctgc gcaagccgat cttggcgcga
     1981 gcccggcgat acgcggcaat ccaggagcgg ttgatctcac cggaaggcac gttccccgcc
     2041 atcgggcgat cgctggcgta tcgcttcggc gtgtttcaac tgctcggaca gatggcgctg
     2101 cggcgggagc tgccggcggc ggtgtcgccg tgccaggtgc ggtgcgcgat gactgcggtc
     2161 atccggcgaa tgatcgaagc gccggggacg ttcgacgagc atggcttcct gacgatcggc
     2221 gtgtgcgggc atcaagcggg gatgggcgag ggctacatct cgacgggcag tttgtacttg
     2281 tgcacgaccg ggctgctgcc gctgggtttg ccggtggggg atgagttctg gacgtgtgag
     2341 gcgagcgatt ggacgcagaa gcgcgtgtgg gcgggggcgg atgtggggac ggatcatgcg
     2401 attgaggaat agctgcgaaa tgcgaatggc gaaatccgaa tccccgaatg aaatccgaat
     2461 ttcgaatgac gaatgaatgc gaagacacta accgctgaag cggttagtcc gggcggaggc
     2521 ttcgcgcgtt tgtgcgtgtc ttcattcgga tttcgtcatt cgtcattcat tcggggattc
     2581 gagtttcgtc attcggcatt caactatgct ctgcagcgcg gcgcacttca tcgaacaatc
     2641 cctgcgtgcc ttcgcggcca tgacctagag cctgcgcttg tttgaaaagg cttgtcacca
     2701 gctcgaccgc tgggagatgc aggcctaagt cggcggcggc ttcctggacc aggcgtaggt
     2761 ccttgagctg aaggtcgatc atgaagccgg gcttgcggtc gccggtgatc atcctggggc
     2821 ccaggttgct cagttgccat gacccggcgg caccggcggc gacggcttct atggtctgct
     2881 tgccgtccag gtttcctgtg gtcgcgagcg tcagcgcttc gcagacggcg aggttggtga
     2941 cgctgacgag gatctggttg accagcttcg tcagctgccc gctgccggcg gggccgcagt
     3001 gggtgatggt tttgcccagg tgttggagca gggggagggc gcgatcgaac gctgggcgat
     3061 cgccgccgac catgatggcg agggtggcgt tgcgggcgcc gacgtcgccg ccgctgacgg
     3121 gggcgtctag gagcgtcgct tggtgctcgg cgagtcgctg ggcgaatcgc tttgtcgcgg
     3181 tgggggagat ggtggagtga tcgatgacga tcgtgtgggg gcggatggtt tcgaagacgc
     3241 cgtggttggc gaagaggacg gcttcgacat cgggggtgtc ggtgacgcag atgaagacga
     3301 catcggcgtg agccgccgcg gcggcggggg tgtcggacca agtcgcgccg gctttcagga
     3361 gttcatcggc tttggatcgc gtgcgattgt ggacggtgac ggcatggccg gcgcggagca
     3421 gatgggcggc catggggtta cccatgatgc ccagaccgat gaaggcgagg cgctgtgggg
     3481 tttgcatgcg gggattgtgg gaggggaggg gcgcgcgatc aacccatcga agccgcgggc
     3541 gaaagccagc ggggtcttag gaatatcaac cgggtagggg actggaaagc ccccgctggc
     3601 tgtcgcctgc ggctattaag ggggattgga acgcctgctc acttcttcgt cagcaccatc
     3661 accacgatca ggatcaggtt gatcagcagc gacacgccca ccgcggcgag gagcatgccg
     3721 gcgtaggggt tctccatcag ttgcgagaag gtcgaaccga cgccgtcgga ggggtgattt
     3781 agatcgaccg tctttccggt ctcttcgatg ctggcgagcg cttccaaatc gaccgcgcgc
     3841 ttggcgtgtt gcgggggatt gccatctgcg acggctcgca ggtcttccag catgtcttcg
     3901 gtgctgccgt agcgctcgtc gcggttcttg gccatcgcga cttcgatgat ctcaccgacg
     3961 ccggaggaaa gggcggtgtt gatgtgatcc ggcggcacca gcggttggcg gaggtgcttg
     4021 tgcatcacgg cgctgggcgt gtcgccttcg aacggcggac ggccggtgac caggtgatac
     4081 atcgtcgcgc ccagcgaata gacgtcagcg cggaagtcga tgtcgacgtc gccgcggatc
     4141 tgctcggggc tgatgtagta cggcgtgccg tacgctttgc cggcttcgct ttcggcggcg
     4201 tgcttgtcgt cggtcgcgcg agccagaccc aggtcagtca acttggcgac gccttgcgga
     4261 gttaggatga tgttcttcgg tttgacgtcg cggtggatca acccgcggcg atgcgcgtgg
     4321 gccagtgcgt cggccatctg gatgcagatg tcgagcgctt c
//
LOCUS       DATIMH010001183         6398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_277656, whole genome shotgun sequence.
ACCESSION   DATIMH010001183 DATIMH010000000
VERSION     DATIMH010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277656"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VLI90_20375"
     CDS             <1..714
                     /locus_tag="VLI90_20375"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HSV16632.1"
                     /translation="EVARIIRKYNYLAVPVVNVRHQLVGLITVDDVVRVLEHEATEDV
                     QKMFGAGAEERLASPWQFSFKKRVWWLEVNLLTAFMAASVVSLFTKTIEALPILAAYQ
                     TIVSGMGGNAGAQAMAVSIRGIALGEVDRRLLKKILYREFIVGLLTGIVIGTTTGLIA
                     AVFHYHEGHSVMLGFVIFLALVLNHVNACLTGVAIPFVMKYFGFDPAQSATIFATTFT
                     DCGGFFVTLWLAKVILHLN"
     gene            872..1252
                     /locus_tag="VLI90_20380"
     CDS             872..1252
                     /locus_tag="VLI90_20380"
                     /inference="COORDINATES: protein motif:HMM:NF042735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSV16633.1"
                     /translation="MSDTMQVGKRLVELCKQGKNVQAVEELYSPNIVSIEAIANPQMP
                     QRMEGKAAIKGKNEWWVNNHEIHGGETNGPWPHGDRFIVHFQYDVTAKEGPMKGKRMQ
                     LEEAGLYTVKDGKIVQEEFFYAMG"
     gene            complement(1268..1969)
                     /locus_tag="VLI90_20385"
     CDS             complement(1268..1969)
                     /locus_tag="VLI90_20385"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSV16634.1"
                     /translation="MVAASRVSDPFPPPAEWVAARRASQIILTIKDVEAMVKAGIVPE
                     DASTELLHGVLLYTDRGATSDPDLDPQQYVERFDLKGSAIVEGADHNYVVSGLAELAL
                     SINSPLRHLRTQSTCVCSETHAPVPDAVILRGPRTDYRGKRPSAADAFCVVEVADSSY
                     ERDAGEKLFGYARANVRQYVISNLRNRTAEVYTEPDVAAGTYAPPQIITGDQSLSLRV
                     GDAERFTVALANVLP"
     gene            complement(2364..4097)
                     /gene="thiC"
                     /locus_tag="VLI90_20390"
     CDS             complement(2364..4097)
                     /gene="thiC"
                     /locus_tag="VLI90_20390"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HSV16635.1"
                     /translation="MPTAPSTDRSYTLPPLGDNPSTERQGTTPGSFALKGRVGTPYTY
                     SSPDTQGMPGVSDITAMDFARAEDAEGKTQLEEARIGRVTDQMKHVATREPHLTAEQI
                     RVEVATGRMIIPANKVHLKYKLEPMAIGRASLTKINANMGASPISSGTEEEVEKLKWA
                     ERWGADTVMDLSTGGDLDACRDAIIRNSTVPIGTVPIYSMIIGKRIEDLTYDVILKEL
                     EKQAKQGVDYFTIHAGVLREHLPFVKKRLIGIVSRGGSLLAKWMLTHNKQNPMYEIFD
                     DICDIMREYDVSFSLGDGLRPGGLADASDEAQLRELQTLGELTERAWAKGVQVMIEGP
                     GHVPFDQIEYNMKLQRKLCHGAPFYVLGPLVTDIFPGYDHITSCIGATSAAYHGASML
                     CYVTPKEHLGLPKKDDVKQGCVAYKIAAHAADVALGIPGSRDRDDELTKARAALNWQK
                     HFDLSFDPDLARAYHDEDLEVDTDFCAMCGHDWCSVRISKEITDFYSGKDEESQPEKK
                     AIASPGISSAGVDVLKQRGVLSQEEIMKLAHKGKKAVCHSDNVQDAEEAKLVQVNTLK
                     AHGVVVNESLI"
     regulatory      complement(4181..4286)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4396..5019
                     /locus_tag="VLI90_20395"
     CDS             4396..5019
                     /locus_tag="VLI90_20395"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HSV16636.1"
                     /translation="MFNLLPKDTVFFDLFEGLARHAISCAEHLRQLGKQFPAVDNEIQ
                     RIRQEEHQADDLTHKALERLDQTFITPFDREDIHELVNGIDDIIDTIDALAKRFPIYH
                     ITTMPNSFRQQTDVLVQACIAMSDAVHRLRKTRKLSELKDALIEIHRLESVGDDVNHA
                     AISNLFAGETEPLEVMKWKELYDYIEEAIDGCEDVGNTLERIVLKNG"
     gene            5205..6257
                     /locus_tag="VLI90_20400"
     CDS             5205..6257
                     /locus_tag="VLI90_20400"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HSV16637.1"
                     /translation="MHPVSLGLIFFIIAVALVFDFLNGFHDAANSIATVVSTRVLTPT
                     QAVIWAAFFNFVAAFIFGTGVASTIGKGLVDTNAIDVYVVFGGLMGAIFWNIITWLLA
                     LPTSSSHALVSAIAGAAIAKSGFKVIIIGKGWGMLLLFLFLSPMIGFILGALNMIVVS
                     WIFRRSTPHKVDTLFRRLQLLSAGVYSLGHGGNDAQKTMGIIVLLLTAAGHEKWSYIG
                     QVHLPWIHHEYGMPIILACHFAIAMGTIFGGWRIVKTMGSGITRLAPVGGFCAELSAA
                     TTIIGATLGHIPISTTHAITGSILGVGTTHGVRSVRWIWGQRIVMAWILTLPCAAFMA
                     AVTYLLIHVLVEPMFR"
BASE COUNT         1241 a         1924 c         1910 g         1323 t
ORIGIN      
        1 gaggtggcgc ggatcatccg gaaatacaac tacctggcgg tgccggtggt gaacgttcgg
       61 caccagctgg tggggctgat cacggtggac gacgtggtgc gtgtgctgga acacgaggcc
      121 accgaggacg tgcagaagat gttcggcgcc ggcgccgaag aacgcctggc cagcccctgg
      181 caattcagtt tcaaaaagcg cgtgtggtgg ctggaggtga acctgctgac cgcgttcatg
      241 gccgcatccg tcgtcagcct cttcaccaag acgatcgaag ccttgccgat cctcgcggcg
      301 tatcagacga ttgtcagcgg catgggcggg aatgccggcg cgcaggcgat ggccgtctcc
      361 atccgcggca tcgcgctggg tgaagtcgat cgccggttgc tgaaaaaaat cctctatcgc
      421 gaattcatcg tggggctgct gaccggtatc gtaatcggca ccaccaccgg cctcatcgcc
      481 gccgtgtttc attaccacga aggccacagc gtgatgctgg gcttcgtcat cttcctggcg
      541 ctggtcttga accacgtcaa cgcctgcctg actggcgtgg cgattccctt cgtaatgaaa
      601 tactttggct tcgatcccgc gcagtcagcg acgatcttcg caacaacctt caccgactgc
      661 ggcggattct tcgtcacgct ctggctcgcg aaggtgatcc ttcatctaaa ctgaacgagc
      721 ctctgaacgc tcttcaatgg ccgtcaatcg gctgttgaat gttgaacgat caaatccgcg
      781 caaaagatta acaaaccgcc agtgcagcca tgctaagata ccccgtaggc gtcaatcgat
      841 tgaccctatt gcgcaaagga aaggtgatcc aatgagcgac acaatgcaag tcggcaaacg
      901 gctggttgaa ctctgcaagc agggaaagaa tgtgcaggcg gtggaggagt tgtacagccc
      961 gaacatcgtc agcatcgaag cgatcgcaaa cccgcagatg ccgcagcgga tggaaggcaa
     1021 agcggccatc aaaggcaaga acgaatggtg ggtgaacaac cacgaaatcc acggcggcga
     1081 aaccaacggc ccctggccgc acggcgatcg gttcatcgtg catttccagt acgacgtgac
     1141 ggccaaggag gggccgatga agggcaagcg gatgcagctg gaggaggcgg gtctgtatac
     1201 ggtgaaggat gggaagatcg tgcaggagga gttcttctac gcgatgggtt gagacgaaga
     1261 ccgtcgatca cggcaacacg ttcgcgagtg caacggtgaa gcgttccgcg tcgccaacgc
     1321 gcagcgacag ggattggtcc ccggtgatga tctgcggcgg cgcgtatgtt ccagcggcga
     1381 cgtcaggctc tgtataaact tccgccgtcc gattgcgcag attgctgata acgtactgcc
     1441 gtacgttcgc gcgggcatag ccgaaaagct tttcaccggc atcacgctcg tagctgctgt
     1501 cggcgacttc gacgacgcag aacgcgtctg ccgcgctagg ccgcttgcct cgatagtcgg
     1561 ttctgggccc gcgcaggata acggcatctg ggaccggtgc gtgtgtttca gagcaaacac
     1621 atgtgctctg cgttctgaga tgccgaagag gtgagttaat ggaaagtgcc aattcggcca
     1681 gccccgaaac cacgtagtta tggtcggctc cttccacaat ggcgcttcct ttcaaatcga
     1741 atcgctcgac gtactgttgg ggatccaaat caggatcgct tgtggcccct cgatcggtgt
     1801 acagcagcac gccatgcagc agctcggtag aggcatcttc ggggacaatt cctgctttaa
     1861 ccatcgcttc gacatctttg atggttagga tgatttgtga agcgcgacgc gcggccaccc
     1921 actcagccgg aggtgggaac ggatcagata ctctggaggc tgcgaccata ggagcaatat
     1981 atccagaagc cctcagggtg atagcttatc cagccaataa gcccgtcgaa ggacccaaca
     2041 tccgactcga tacctgcaaa atccaatgcg gacccgcaag cagcgcgatg gcttatctac
     2101 gcaatggctt tcgcgtggtt cctgttcggc agtaccgtga cagcccacga gacgccggct
     2161 tggtcgttgc cgaagaaaat cacttgcaca ttcagcgctc tcgtcatctt cgtgctgctg
     2221 atcgtggcgc gccgcaaggg tggcatcggc tgtgcgcttc tgttctttct atgtggcgca
     2281 ttcatcgtcg gcgccgtttg gtgtcatgtc cacacgctta gaaacgacgg cacaatctca
     2341 acccctgagc taggtccgct gcatcagatc aatgattcgt tcaccactac gccgtgcgct
     2401 ttcagcgtgt tcacctgcac caacttcgcc tcttcggcgt cctgcacatt atcgctgtgg
     2461 cacaccgcct tcttgccctt gtgggcgagc ttcatgattt cttcctgact tagcacgccg
     2521 cgttgtttga gcacatcgac ccccgcggag gaaatgcccg gcgaggcgat cgccttcttc
     2581 tcgggttggc tttcctcgtc cttgccgctg tagaagtccg tgatttcctt gctgatgcgg
     2641 acgctgcacc agtcgtggcc gcacatggcg cagaagtcgg tgtcgacttc caggtcttcg
     2701 tcgtgatacg cgcgggcgag atcggggtcg aagctcaggt cgaaatgctt ttgccagttc
     2761 aacgcggccc gcgctttggt caactcgtca tcgcgatctc ttgagccggg gatgcccagc
     2821 gccacgtcgg cggcgtgggc ggcgatcttg tacgcaacgc agccctgctt cacgtcgtcc
     2881 ttcttcggca atcccaggtg ctccttgggc gtcacataac agagcatcga agcgccgtgg
     2941 tacgcagcgc tggtcgcgcc gatgcagctg gtgatgtggt cgtagccggg gaagatgtcg
     3001 gtgactaatg ggccaagcac gtagaacggc gcgccgtggc aaagcttgcg ctgcagcttc
     3061 atgttgtatt cgatctgatc gaacggcacg tgcccggggc cttcgatcat gacctgcacg
     3121 cctttggccc acgcgcgctc ggtcagctcg ccgagcgttt gcagctcgcg cagctgcgct
     3181 tcgtcggaag catcggccaa gccaccgggc cgcaagccat cacccaacga gaacgaaaca
     3241 tcgtactcgc gcatgatgtc gcagatatcg tcgaagatct cgtacatcgg attctgctta
     3301 ttgtgcgtca gcatccactt ggccagcagc gaaccgccgc ggctgacgat gccgatcaat
     3361 cgcttcttca cgaacggcag atgctcgcgc agcacgccgg cgtggatcgt gaaatagtcc
     3421 acgccctgct tcgcctgctt ttccagttct ttcaggatca cgtcgtacgt gaggtcttcg
     3481 atgcgcttgc cgatgatcat cgagtagatc ggcacggtgc cgatcggcac ggtgctgttg
     3541 cgaatgatcg cgtcgcggca ggcgtccagg tcgccgccgg tgctcaggtc catcaccgtg
     3601 tccgcgcccc agcgctcggc ccacttcagc ttttcgactt cctcttccgt gccggatgag
     3661 atgggcgatg cgcccatgtt ggcgttgatc tttgttagcg atgcccggcc gatggccatc
     3721 ggttccagct tgtacttcag gtgcaccttg ttggcgggga tgatcattcg cccggtggcg
     3781 acttcaacgc ggatctgctc ggccgtcagg tgcggttcgc gcgtcgcaac gtgcttcatc
     3841 tgatccgtca cgcggccgat gcgcgcttct tccaactgcg tcttgccttc ggcatcttcc
     3901 gcgcgggcga aatccatcgc ggtgatatcg gaaacgcccg gcatgccttg tgtgtcgggg
     3961 gaagagtaag tgtatggcgt gccaacgcgg cccttgagcg cgaacgagcc gggcgtggtt
     4021 ccttggcgct ccgtcgaggg attgtcgcct aaagggggaa gcgtgtagga acgatcggtg
     4081 gatggggcgg tgggcatgtg aaaaactcca agtccgtccc acggcatagc cgtgggcttt
     4141 ggtgattact tcgacttcta ttgggtggtg cgcgcggtcg gaagacgtgc tttcgctttc
     4201 ctacgccggc atcacccgga tcaggtctaa gggtttgatc tcagatcgcc tcaatgttcc
     4261 gcgggcgatc acccctagcg aatctcaagc agagtttagc gccactggca tgttcaagca
     4321 agggcagatc gtttgggatt gccggccttc gcccgtacaa tccgccgccc ccgctgctcg
     4381 ggcgggcaac aacgcatgtt caacctgcta cccaaagaca ccgttttctt cgacctcttc
     4441 gaaggcctcg ctcgccacgc gatctcctgc gccgagcatc tccgccagct gggcaagcaa
     4501 ttccccgcgg tggacaacga gatccagcgc atccgccagg aagagcacca ggccgacgac
     4561 ctcacgcaca aggcgctgga gcggctggac cagacgttca tcacgccgtt cgatcgcgag
     4621 gacatccacg agctggtcaa cggcatcgat gacatcatcg atacgatcga cgcgctggcc
     4681 aagcgatttc cgatctatca catcacgacg atgccgaatt ccttccgcca gcagaccgac
     4741 gtgctggtgc aggcgtgcat cgcgatgagc gacgcggtgc atcggctgcg caagacgcga
     4801 aagctgtcgg aactgaaaga cgcgctgatc gaaatccacc gcctggaaag cgtcggcgac
     4861 gacgtcaacc acgcggccat cagcaacctg ttcgccggcg aaaccgaacc gctggaagta
     4921 atgaagtgga aggaactgta cgactacatc gaagaggcga tcgacggctg cgaagacgtg
     4981 gggaacacgc tggagcggat cgtgttgaag aatgggtgag tcttacacct tgtcctgagc
     5041 gcaggcggtg ctccgccgga gtcgaaggat atagcctcga acgtttgtga acctcagtca
     5101 acccgaaaga gtcatagtca cttgcgaagg cgataagttc accgacgttc gaggctagat
     5161 ccttcgactc cggcgtgtac gcctccgctc aggataaatt caaaatgcac cccgtcagcc
     5221 tcggcctgat cttcttcatc atcgcggtcg cgctggtatt cgacttcctc aacggcttcc
     5281 acgacgccgc caactccatc gccaccgtcg tctccacgcg cgtgctcacg ccgacgcagg
     5341 ccgtcatctg ggctgcgttc tttaacttcg tcgcggcgtt catcttcggt accggcgtgg
     5401 ccagcacgat cggcaagggg ctcgtcgata ccaacgccat cgacgtctac gtcgtcttcg
     5461 gtggattgat gggcgcgatc ttctggaaca tcatcacctg gctgctcgcg ctgccgacca
     5521 gctcatcgca cgcgctggtc agcgccatcg ccggggcggc catcgccaag tcggggttca
     5581 aggtcatcat catcggcaag ggctggggga tgctgctgct gttcttgttc ctctcgccga
     5641 tgatcggctt catcctgggc gcgctcaaca tgatcgtggt ctcgtggatc ttccgccgat
     5701 cgacgccgca caaggttgac acgctgtttc gccgcctgca gctgctcagc gccggcgtct
     5761 acagcctcgg tcacggcggc aacgacgccc agaaaacgat gggcatcatc gtgctgctgc
     5821 tgacggcggc cggacatgaa aagtggagct acatcggcca ggtgcacctg ccgtggattc
     5881 atcacgaata cggcatgccg atcatcctcg cctgccactt cgcgatcgcg atgggcacca
     5941 tcttcggcgg atggcgcatc gtcaaaacga tgggcagcgg catcacgcgc ttagcgccgg
     6001 tgggtgggtt ctgcgctgaa ttgtccgccg cgacgaccat catcggcgct accttgggtc
     6061 atatccccat ctcgaccacg cacgcgatca ccggttcgat cctcggcgtc ggcacgacac
     6121 acggcgtgcg ttccgtgcgc tggatctggg gtcaacgcat cgtgatggcg tggatcctca
     6181 ccctcccctg cgccgccttc atggcggcgg tgacttatct gctgattcac gtcttggtcg
     6241 agccgatgtt tcgatgaaaa aaaactaggg tggcaaaatc gacttgagcg aatgtagtgt
     6301 gggccggaca gccatggagg tgctgtatgg cacggatcgc cgcccgcccc acgccgacga
     6361 tggaacaagt gctcgaaccg ctgagcgatc gctgccgg
//
LOCUS       DATIMH010001184         1701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_277691, whole genome shotgun sequence.
ACCESSION   DATIMH010001184 DATIMH010000000
VERSION     DATIMH010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277691"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..311
                     /locus_tag="VLI90_20405"
     CDS             33..311
                     /locus_tag="VLI90_20405"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HSV16638.1"
                     /translation="MELLADLCKRHALRELSVFGSALRRDFRPDSDVDILIELQPGQT
                     MTIERFVAIRDELKAMFHRDVDLVEKPLVQNPYRRGEILRTREVLYAA"
     gene            313..663
                     /locus_tag="VLI90_20410"
     CDS             313..663
                     /locus_tag="VLI90_20410"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HSV16639.1"
                     /translation="MQDAALPLDMLESARRVLTYCAGRSRADFDADLMFADAVVRRVE
                     IVGEAARGVSDQGRSDHPEIGWHPIMATRHILAHEYATVNYDIVWRIVTDHMPRLVTQ
                     LEGILAQAPPPPPP"
     gene            671..1393
                     /gene="bioD"
                     /locus_tag="VLI90_20415"
     CDS             671..1393
                     /gene="bioD"
                     /locus_tag="VLI90_20415"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HSV16640.1"
                     /translation="MLSPINIPGLFITGTDTGVGKTLIAAAIADFFRRRGARVGVCKV
                     AATGCVKRREGLVSEDAEFLAHAADSSFPLDVICPQRYAEPLAPAVAAERAKQPLEWA
                     SIQRSLDMIAAGSDLLIVEGVGGLMVPMDKQSTVLDVARWLDLPTVIVARPALGTINH
                     TLLTVNALRAAKVPVVGVVINRYPAENPDTAEETNPRAIERWGKVNVLCLTPDEAFKA
                     PNIPDGVVAAIDAVDWGRLASE"
BASE COUNT          319 a          492 c          558 g          332 t
ORIGIN      
        1 ccccgctcat cgaagaacgc ttcggcgtgt cagtggaatt gctcgccgat ttgtgcaaac
       61 gtcacgcgct gcgtgagctg tcggtcttcg ggtcagcgct gcgacgcgac ttccgccccg
      121 atagcgatgt ggacatctta atcgagcttc agccggggca gaccatgaca attgaacgtt
      181 ttgtcgcgat tcgtgatgag ctgaaggcga tgttccaccg cgacgttgat ttggtcgaga
      241 aaccgctggt tcaaaacccc tatcgccgcg gcgagatcct gcgcacccgc gaggtgctgt
      301 atgccgctta gcgtgcagga tgcggcgctg cctctcgaca tgctcgaatc ggcacgccgt
      361 gttctcacgt attgcgcggg tcgatcacgg gctgattttg atgcggatct gatgtttgct
      421 gacgccgttg tacgtcgcgt cgagatcgtt ggtgaagccg cccgcggtgt ttccgatcag
      481 ggcagatcag atcatcccga gatcgggtgg catccgatca tggcgacgcg tcacatcctc
      541 gcgcacgaat atgctaccgt gaattacgac attgtttggc gaatcgtgac ggatcacatg
      601 ccgcggctgg tcacgcagct cgaaggaatt ctggcgcaag cacctccacc tccgccgccg
      661 tgagtatttc ttgttatcgc ccattaatat ccccggcctg ttcatcaccg gcaccgacac
      721 cggtgtcggc aagacgctca tcgcggcggc gatcgcggat ttctttcgcc ggaggggagc
      781 acgcgtcggc gtctgcaaag tcgcggcgac tggttgcgta aagcggcgtg aaggactcgt
      841 cagcgaagat gcagaatttc ttgcccacgc ggcggattcg tcgtttccgc tggacgtgat
      901 ctgcccgcag cgctacgccg aaccgctggc acccgcggtg gcggcggagc gcgcgaagca
      961 gccgctggag tgggccagca ttcagcgatc gctcgacatg atcgcggcgg gcagcgatct
     1021 tctcatcgtc gaaggcgtcg gcgggttgat ggtgccgatg gacaagcagt cgacggtgct
     1081 ggatgtcgcg cgatggctcg atctgccgac ggtcatcgtt gcgcggccgg cgctggggac
     1141 gatcaatcac acgctgctga cggtgaacgc gctgcgggcg gcgaaggtgc cggtcgttgg
     1201 cgtcgtcatc aatcgctatc ccgcggagaa cccggacacg gccgaggaaa cgaatccgcg
     1261 cgcgatcgag cggtggggga aggtcaacgt gctatgcctg acgccggacg aggcgttcaa
     1321 agcgccgaat attcccgatg gcgtcgtcgc cgcgatcgac gcggtggact ggggacggct
     1381 cgcgtcggaa taacaaaccg tcaggcctgg tcacttcagt gaccagattt ttcccctcgc
     1441 acaatcgcag acctggtcac tgaagtgacc gggtgcgtga gtgattcggc gagtcctggg
     1501 ccaaacatga tacgagccgc gcacggagtg tactcacgta gtaagcgggc agaggcggtg
     1561 aaaaaaacta gggtggcaaa atcgacttga gcgaatgtag tgtgggccgg acagccatgg
     1621 aggtgctgta tggcacggat cgccgcccgc cccacgccga cgatggaaca agtgctcgaa
     1681 ccgctgagcg atcgctgccg g
//
LOCUS       DATIMH010001185         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4029773, whole genome shotgun sequence.
ACCESSION   DATIMH010001185 DATIMH010000000
VERSION     DATIMH010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4029773"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VLI90_20420"
     CDS             <1..405
                     /locus_tag="VLI90_20420"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HSV16641.1"
                     /translation="VPAVLGARAGYGLPYYWSQMRVARSAREMKYQSRRRRPQRNAVT
                     DITIIEKDPIEANDLEIFLTARFRLYSRLFGRLIYADVEHEPWPLRSAESLAVKQTLI
                     QAAGLPSPADAPLVHFSRGVHVRIGRPKLLTC"
     gene            complement(402..1223)
                     /locus_tag="VLI90_20425"
     CDS             complement(402..1223)
                     /locus_tag="VLI90_20425"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HSV16642.1"
                     /translation="MTHADKAGCSRKATAILIGGAEQKTGERTILREVAARAGTSPLV
                     VCTVGSTIPGQMFGVYKRAFAGLGVTDVVHVPVGKREAADDVGAAEAVGLAKVFFFTG
                     GDQLRITSMLGGSRLWDAVQRMYQRGGTVAGTSAGASALGHTMPISAEADEHRVSAAS
                     SLLPALGLLKDLIVDQHFAQRGRMGRLIAGVSENPRLLGIGIDENTAAVWTAGVFRVI
                     GSGAVYVVDGREVSRSNVAGAELQCAMSTFDLRLHVLSSQDCFDVAKRRPFSKSG"
     gene            1292..>1528
                     /locus_tag="VLI90_20430"
     CDS             1292..>1528
                     /locus_tag="VLI90_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16643.1"
                     /translation="MNGNHLIGLRIRKIGVRDRAPVDYVHGAIPDDIEVVPKPRDDRR
                     ILVDADADQPRILRHRAEQATDAPALCEVLVLNQI"
BASE COUNT          273 a          546 c          450 g          259 t
ORIGIN      
        1 gtgcccgccg tgctgggagc gcgcgccggt tacggcctgc cttactactg gtcgcaaatg
       61 cgtgtggccc gcagcgcccg tgagatgaag taccaaagcc ggcgccgccg gccgcagcgc
      121 aatgcagtca cggacatcac catcatcgag aaagacccga tcgaagccaa cgacctcgag
      181 atctttctca ccgcgcgctt ccgcctctac tcccgcctgt tcggccgctt gatttatgcc
      241 gacgtggagc acgagccctg gcctctgcgg tcggccgagt ctcttgcagt gaagcagacg
      301 ctgatccagg cagccggcct gccttcgccg gcagacgcgc cgctggtcca cttctcccgc
      361 ggcgtccacg tgcgtatcgg ccgcccgaaa ctcctgacgt gctagccgct tttggagaag
      421 ggacggcgct tggcgacgtc aaagcaatcc tgcgagctca agacgtgcag ccgcaaatcg
      481 aacgtcgaca tggcgcactg cagttcggcg ccggcgacgt tcgacctcga cacctcgcgc
      541 ccgtccacga cgtacactgc gcccgacccg atcacgcgga atacgcccgc cgtccaaacc
      601 gcggcagtat tctcatcgat accgatgccc agcagtcgcg gattttcgga gacgcccgcg
      661 atcagacggc ccatgcgccc gcgttgcgca aagtgctggt cgacaatcag atccttgaga
      721 agcccgaggg cggggagcag gctcgacgcc gccgacacgc gatgctcgtc cgcttctgcc
      781 gagatcggca tcgtgtgccc gagcgccgaa gccccggcgg acgtgcccgc taccgtgccg
      841 ccgcgctgat acatgcgctg taccgcatcc cacagccgcg acccgcccag catgctcgtg
      901 atgcgcaact ggtcgccgcc cgtgaagaag aagaccttcg ccaagccgac tgcctcggcc
      961 gcgcccacgt cgtcggcagc ttcgcgtttg cccacgggga catgcactac gtcagtcacg
     1021 cccaacccgg cgaacgcgcg cttataaacg ccgaacatct ggcccggaat cgtactgcca
     1081 accgtgcaca ccaccagcgg actagtcccg gcgcgtgcgg ccacctcgcg gagaatggtt
     1141 cgctcgccgg tcttctgttc cgccccgcca atcagtatgg cggtcgcctt ccgggagcag
     1201 cccgctttgt ccgcgtgcgt cactggcgca tcgcagtcct tgtcgtgagg tcgaaacgat
     1261 ccccgcgact caggacgtcg agtttcacgt tgtgaatgga aatcacctca tcggtcttcg
     1321 tatccgaaag attggtgtac gtgatcgagc gcccgtcgat tacgtacacg gcgccattcc
     1381 cgatgatatc gaggtagtgc ccaagccgcg cgacgaccgc cgtatcctcg tcgatgccga
     1441 cgccgatcag ccgaggattc tgcgccaccg cgccgagcag gcgactgacg cgcccgcgct
     1501 gtgcgaagtg ctggtcctaa atcagatc
//
LOCUS       DATIMH010001186         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2879535, whole genome shotgun sequence.
ACCESSION   DATIMH010001186 DATIMH010000000
VERSION     DATIMH010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2879535"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VLI90_20435"
     CDS             <1..256
                     /locus_tag="VLI90_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16644.1"
                     /translation="YGYSGAWFTSMEGDLNDCYLRSYSFNKAKSVAVHELGHQISLDH
                     LGPLPCANDPIMDPYTSSRYDTCGHSTPQWDDINGVNAHY"
     gene            265..894
                     /locus_tag="VLI90_20440"
     CDS             265..894
                     /locus_tag="VLI90_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16645.1"
                     /translation="MSMTRRNFALVAAALFVAVGLAVTVAAARGATGTAASVAPAHAG
                     LRHVTIESSWAHAYGDLHELKAGAIAVVLGTVMSGQQLATSSFGVNTPTKEFQIHVER
                     TLKGSVPTTVAVQQIGGTTADGTTVVEVSDDPLLAAGERGIFFLFTSNGAFYTLLDGG
                     MGHFTSANGIVDQRGAFAASPAFIRSGRQPAAPIHGVSEAAFVAQVLAA"
     gene            991..>1587
                     /locus_tag="VLI90_20445"
     CDS             991..>1587
                     /locus_tag="VLI90_20445"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSV16646.1"
                     /translation="MAGVTALVPPQTRAAGTVVGVKTYYLALGDSLAFGYEPNLDWSH
                     GYAQKWNADLRQHGSTSFTNYGCNGETSATFINGGCPFAFALHNFYADAQLNAAVKFL
                     TAHPGQVSPVSLDIGANDLLPDINSSTCAISSTWATDLANLDARLTGTILPKLAAALT
                     VNGARSGDLVMMNYYDPFQNQCASNPQVLANLQTLNQHI"
BASE COUNT          301 a          500 c          505 g          281 t
ORIGIN      
        1 ctacggctac tcaggcgcct ggttcacgag tatggagggc gacttgaacg attgctacct
       61 ccggagctat tcattcaaca aggcaaagag cgtcgccgtc cacgaattgg ggcaccagat
      121 cagcctcgac catctggggc cgctgccttg cgccaacgat ccgatcatgg acccatatac
      181 aagcagccgc tatgacactt gcgggcacag cacaccacag tgggatgaca tcaacggcgt
      241 caacgcccat tactaaggag gtttgtgtcc atgactcgcc ggaactttgc cctcgtggcg
      301 gccgctctat tcgtggcggt tggcctggcc gtcaccgttg ccgccgctcg tggtgccaca
      361 ggcaccgctg cctcggtagc ccctgcacat gccggcctgc ggcatgtgac gatagagagc
      421 agttgggcgc atgcctacgg cgacctgcac gaacttaagg ccggcgcgat tgctgtcgtg
      481 cttggcacag tgatgagcgg ccagcagttg gcaacctcca gtttcggcgt caacacgccg
      541 acgaaagaat tccagatcca cgtcgagcga acgctgaagg gaagcgtacc cacgacggtt
      601 gccgtccagc agattggcgg tacgacagcg gatggcacga ctgtggtaga agtctcggac
      661 gatccattgc ttgcggctgg cgaacgtggc atctttttcc tcttcaccag caatggagcg
      721 ttttacaccc tgcttgacgg cgggatgggt cacttcacca gcgctaacgg gatcgttgat
      781 caacgcggcg cattcgcggc atcgccagct ttcatcagat cgggccgcca acccgccgcg
      841 ccgatccacg gggtctccga ggccgccttc gtcgcgcagg ttctcgccgc gtagacgctg
      901 cggcgacatg acgcggaccc cacgcggcat cctcggcggt tctcctcgac aggcggcggc
      961 agatggcggc ggttcgcgct ggccgtggcg gtggcaggcg tcacggcgct cgtcccaccg
     1021 cagacgcggg ccgcgggaac ggtggtgggc gtgaagacgt attacctggc gctgggcgat
     1081 tcgctggcgt tcggctacga gccgaacctg gactggagcc acggctacgc gcaaaagtgg
     1141 aacgcggacc tgcggcagca cggcagcacg agcttcacga attacggctg caacggcgag
     1201 acgagcgcga cgttcatcaa cggcggctgt ccgttcgcgt tcgcgctaca caacttctat
     1261 gctgacgccc agctcaacgc ggcggtcaag tttctgacgg cgcatcccgg tcaggtcagc
     1321 ccggtctcgc tggacatcgg agcgaacgac ttgctgccgg atatcaatag ctcgacctgc
     1381 gcaatcagca gcacgtgggc gaccgacctg gcgaacctgg atgcgcggct gacgggaacg
     1441 atcctgccga agctggcggc ggcgctgacg gtgaacggcg cgcggtcggg cgatctggtc
     1501 atgatgaact actacgatcc gttccagaat cagtgcgcga gcaatccgca ggtgttggcg
     1561 aacctccaga cgctgaacca gcacatc
//
LOCUS       DATIMH010001187         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1729382, whole genome shotgun sequence.
ACCESSION   DATIMH010001187 DATIMH010000000
VERSION     DATIMH010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729382"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1896
                     /gene="aceB"
                     /locus_tag="VLI90_20450"
     CDS             274..1896
                     /gene="aceB"
                     /locus_tag="VLI90_20450"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HSV16647.1"
                     /translation="MTLHLPPVADPPPHTASLVHVLQPIGPAERQILTGDALHFLGRL
                     ARRFEPQRQFILEQREQRQAELDAGGPLPDFPDETRQLRASSWRVAPIPADLRVRRVE
                     ITGPVDRKMIINALNSGADVFMADLEDSNAPTWRNCIDGQVNLYDAVRRTISYASPDG
                     KQYRLNERTATLMVRPRGWHLEEKHVLVDGRAMSASLFDFGLYLYHNADALTAMGAGP
                     YFYLPKLEGHLEARLWNDVFVWSQRELGIPQGTIRATVLIETILAAFEMDEILWELRD
                     HSAGLNCGRWDYIFSLVKKFSNRAGFVLSDRALVTMTTPFMRAYSQLAIKTCHRRGIH
                     AIGGMAAQIPIKNDATANDAAIAKVRADKLREVTDGHDGTWVAHPGLVPVAMAEFARM
                     PNANQLDRMREDLSVSAADLLTVPQPPTISEDGLRTNIRVGIQYLESWLRGSGCVPIN
                     NLMEDAATAEISRAQLWQWVRHKATLADDRVITLDLVESLVVEELSKVRDQMGPARFA
                     AGQFVRAASLFQRMTESARFPEFLTSIAYREL"
     gene            2012..3322
                     /gene="aceA"
                     /locus_tag="VLI90_20455"
     CDS             2012..3322
                     /gene="aceA"
                     /locus_tag="VLI90_20455"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HSV16648.1"
                     /translation="MSINGKSQIISAQAVEEMAQRWSQDPRWRGVARLYSAADVLRLR
                     GTVQIEYTLARRGAERLWNLLQTEPHIPALGAMTGNQAVQQVQAGLKAIYVSGWQVAA
                     DANNAGQMYPDLSLYPSDSVPNLCRRMNNALLRADQIQHAGGGHEVDWLAPLVADAEA
                     GFGGNLNAFEVMKGMIEAGAAGVHFEDQLSSAKKCGHMGGKVLVPTQEAIQKLIAARL
                     AADVLGVPTVLIARTDADSARLITSAIDQRDRAFLTGARTPEGFYQINGGIDAAIARG
                     LAYAPYADLLWCETSHPDLREAKRFAEAVRHDYPNKMLAYNCSPSFNWRRHLDDAAIA
                     SFQRELGAMGYKFQFITLAGFHALNFSMFSLARSYARDGMTGYCELQEREFLAEPIGY
                     HATSHQRFVGTGYFDEVAKVISGGEASTGAFKGSTEEEQFAAAG"
     gene            3670..>4178
                     /locus_tag="VLI90_20460"
     CDS             3670..>4178
                     /locus_tag="VLI90_20460"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSV16649.1"
                     /translation="MKALKGKVCLVTGGSRGIGKAIALALAEEGANVAIEYAANVTAA
                     AAVVEEIQKRNVQAKAYKANVADAHQVDEMAAAILTDLGAVDVLVNCAGITRDRSFLK
                     LSREAWDEVIATNLTGPFNVAKCVVPTMVERGWGRVINISSIVGQQGNFGQTNYAATK
                     GGLIAFSKAL"
BASE COUNT          772 a         1272 c         1399 g          735 t
ORIGIN      
        1 tgagtcccat ctgaaggcgg catcggtttt gcaaaagcac tatcccgcag ttggcggcat
       61 ctgtcctggg atgctcatgc gtgttcgcgc gagctacagg cgacatgcca gatcattgtt
      121 gcgcgcgcca gcgccctgag cgccgatctc gtgtctgttg gtcgttgata tcgtgcctga
      181 gcgtcgtccc cctgcgccgc gcggaatgtg gcgccaccgc gcgttgtggt ggcggtgtga
      241 cgtcattgcc cgctcggtcg ggcaggagat gttatgaccc ttcatttgcc cccggtggcc
      301 gatccgcccc cgcacacagc ttcgctcgtc cacgtgctcc agccgatcgg acccgccgag
      361 cgccagatcc tcaccggtga tgcgctgcac ttcctgggac gactggcccg gcggttcgaa
      421 ccgcagcggc agttcatcct cgagcagcgc gagcagcgac aggcagaatt ggatgccggt
      481 ggtccgttgc cggatttccc ggacgagacc cggcagctgc gcgccagcag ctggcgcgta
      541 gcgcccattc cggccgacct ccgcgtgcgg cgcgtggaga tcaccggtcc ggtcgatcgc
      601 aagatgatca tcaacgcgct caacagcggc gccgatgtct tcatggccga tcttgaagac
      661 tccaacgcgc ccacctggcg gaactgcatc gacggccagg tgaacctgta cgatgcggtt
      721 cggcggacga tcagctatgc cagccccgat ggcaagcagt atcgcctgaa cgagcgcacc
      781 gcgacgctga tggtgcggcc gcgcggttgg cacctggaag agaagcacgt gctggtggac
      841 ggtcgggcga tgtcagcatc gctgttcgac ttcggtctgt acctctatca caacgccgac
      901 gcgctgaccg ccatgggcgc cgggccgtac ttctacctgc cgaagctgga aggccacctc
      961 gaagcgcggc tgtggaacga cgtgttcgtc tggtcgcagc gcgagctggg cattccgcag
     1021 ggaacgatcc gcgcgacggt gctgatcgaa acgatcctgg cggcgttcga gatggacgag
     1081 attctctggg agctccgcga tcactcggcc gggctgaatt gcgggcggtg ggattacatc
     1141 ttcagcctgg tcaaaaaatt cagcaaccga gcgggctttg tgctgtcgga tcgcgcgctg
     1201 gtgacgatga cgacaccctt catgcgcgcg tattcacagc tggcgatcaa gacgtgccat
     1261 cgccggggca tccatgcgat cggcgggatg gcggcgcaga tcccgatcaa gaacgatgca
     1321 actgccaacg atgccgccat cgcgaaggtg cgggcggaca agctgcgcga ggtaacggat
     1381 ggccacgatg gcacgtgggt ggcgcatccc ggtctggtgc cggtggcgat ggccgagttc
     1441 gctcggatgc cgaacgccaa tcaactggac cggatgcgcg aagacttgag cgtcagcgcg
     1501 gcggacctgc tgacggtgcc gcagccgccg acgatcagcg aagacggcct ccgcacgaac
     1561 atccgcgtcg gcattcaata cctggagtcg tggctgcgcg ggtcgggttg cgtgccgatc
     1621 aacaacctga tggaagacgc cgccaccgcg gagatctcgc gcgcccagct ttggcagtgg
     1681 gtgcgacaca aggcgacgct ggccgacgac cgggtcatta cgctcgatct ggtcgagtcg
     1741 ctggtggtgg aggaattatc gaaggtgcgc gaccagatgg gccccgctcg attcgccgcc
     1801 gggcagttcg tgcgcgcggc gtcgttgttc cagcggatga ccgaaagcgc acggttcccc
     1861 gagttcttaa cgtcgattgc atatcgcgag ctataggcgt gggtgagccg gccgtgtgtg
     1921 cagttgcggg cggaagtgtt ccaccggcaa ctgccagccg ctgcactaaa atagtgacta
     1981 gtgctttggt agcacaaaca aggagacggt catgtcgatc aatggcaaat cgcagatcat
     2041 ttcggctcag gcggttgaag agatggccca gcggtggtcg caagacccgc gctggcgcgg
     2101 cgtcgcgcgg ctttacagtg cggcggatgt attgcgcttg cgcgggaccg ttcagatcga
     2161 atacaccctc gcccgtcgag gcgccgagcg gttgtggaac ctgctgcaga ccgagccgca
     2221 catcccggcg ctgggcgcga tgaccggcaa ccaggcggtg cagcaggtgc aggcgggcct
     2281 gaaagcgatc tacgtcagcg gctggcaagt cgccgccgat gccaacaacg ccgggcagat
     2341 gtatccggac ctgagcctct atcccagcga tagcgtgccg aacctttgcc ggcggatgaa
     2401 caacgcgctg ctccgcgccg accagatcca gcatgccggt ggtgggcacg aggttgactg
     2461 gctcgcgccg ctggtcgccg atgccgaggc cggtttcggc ggtaacttga acgcattcga
     2521 ggtcatgaag gggatgatcg aagccggcgc ggccggcgtg cacttcgaag atcaactgtc
     2581 gtcggcgaag aagtgcggcc acatgggtgg caaggtgctg gtgccgacgc aggaggcgat
     2641 tcagaagctc attgctgcgc gcctggcggc ggacgtcctg ggcgtgccga cggtgctgat
     2701 cgcgcgcacc gacgccgatt cagcgcgatt gatcaccagc gcgatcgatc agcgagatcg
     2761 cgcgtttctc accggcgcgc gaacgcccga ggggttctac caaatcaacg gcgggatcga
     2821 cgccgccatc gcccgcggat tggcgtatgc cccgtacgcc gatctgctct ggtgtgaaac
     2881 gtcgcacccg gatctccgtg aagccaagcg cttcgccgag gcggtgcggc atgactatcc
     2941 caacaagatg ctggcgtaca actgctcgcc cagtttcaac tggcgccggc atttggacga
     3001 cgcggccatc gcgtcgttcc agcgcgagct gggcgcgatg ggatacaaat tccagttcat
     3061 cacgctcgcc gggttccacg cgctgaactt cagcatgttc tcgctcgccc gcagttacgc
     3121 ccgcgacggg atgaccggat actgcgaact gcaggaacgc gaattcctgg ccgagccaat
     3181 cggctatcac gccacgtcgc atcagcgttt cgtcggcacc ggttatttcg acgaggtggc
     3241 gaaggtgatc tccgggggtg aggcatcgac cggcgcgttc aagggcagca ccgaggaaga
     3301 gcagttcgcc gcggcgggtt gatcgccgcg tcgattgcgg aaaagatgcc acgcgcgtgg
     3361 gaaggtgttc ccacgcgcgt ggctgcgtta ttcggaccgt cagatccacg atcataacgc
     3421 ggtggttggg ggcagcctca ggaaaatcga tataaaagga ttaaaagata ttttaatcgt
     3481 gaccggtgcc gcgcgcatcg atatcctgcg atgacaatcc cgtttgcatc gggcttggcg
     3541 tcactaccgc aaactccatt gtttcgcacc atcgtcgcgc gtctatcggc ggccaggcca
     3601 catgcatcac agctcactac acgcgctggc gcgccccgac gggaggcgcg cgagcccggg
     3661 agtctgatca tgaaggcgct caaaggcaaa gtgtgcctcg tgaccggtgg gtcacgagga
     3721 atcggcaagg caatcgcgct ggcgctggcg gaggaaggcg cgaacgtcgc aatcgaatat
     3781 gccgccaacg tcaccgccgc cgccgcggtg gtcgaggaaa tccagaagcg gaacgtgcag
     3841 gctaaggcct acaaggccaa cgtcgccgac gcccaccagg tggacgaaat ggcggcggcg
     3901 atcctcaccg atcttggtgc ggtcgatgtg ctggtgaact gcgccggcat cacccgcgac
     3961 cgctcatttc tgaagctcag tcgcgaggcg tgggacgagg tgatcgcgac gaacctgacg
     4021 ggtccgttca acgtcgccaa gtgcgtggtg ccgacgatgg tggaacgcgg ttgggggcgc
     4081 gtgatcaata tttcgtcgat cgtcgggcag caggggaatt tcggccagac caactacgcc
     4141 gccaccaagg gcgggttgat cgccttcagc aaggcgct
//
LOCUS       DATIMH010001188         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1438850, whole genome shotgun sequence.
ACCESSION   DATIMH010001188 DATIMH010000000
VERSION     DATIMH010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438850"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VLI90_20465"
     CDS             complement(<1..851)
                     /locus_tag="VLI90_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16650.1"
                     /translation="MPWWAKGALKLSLSRIPISYHVRRRLMLAHHGAMTQPAYAHDVF
                     RRHFDAAAFHRKDSGGFTAMELGPGDSLFSAVVAKAHGASATSLVDVGRFASDDVELY
                     RKMAAYLCDRGLPAAPDLRSARTMDDVAAACGLTYATDGLAALRRLPDASIDFTYSNA
                     VLQAVRRDEFLPTLKQLRRLTHPAGCNVHSIDLRDMMALSLNHLRFSPGMWESNLIRQ
                     SGFYTNRLRLAEIIQNCDEAGFTSEISELNQWPALPVNRRRLARPYRDMPDEELRTKT
                     IRIVLRPR"
     gene            complement(1094..2614)
                     /locus_tag="VLI90_20470"
     CDS             complement(1094..2614)
                     /locus_tag="VLI90_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16651.1"
                     /translation="MIAPLKQPLRSEIAQHHQATLDAAAAALRARRERRGAWLAAALG
                     FFALMPYAAISAGNNSSIQFGNLLTIVMAVPTLLLSWRYRSLWFYPLLMAPLVIGMFK
                     VALAGNGDLSLTFKAVLVWFVVALTIVAAQLYAPRHTLAMLTGMAVATIIHAGVGVWQ
                     WYAFDNGEFPMLWAYTTNPSFLSVEQYARTIARYTQRPFGIFPEPSAMSSSLAPWVLF
                     WIAHLTGVIRLRQRAAAWQNALFAAAAVGGLGLIILSRSGHSAITLAAAMTFGIIWLT
                     RCRATFRTFVVLAGVFGVIAPIVIYLAFIALSDRLFGASAMGNSSWEERSSSLVIGFS
                     LWVDHGLIPLFFGIGPGQTTPILQRVYHLEAIWSVLLTYVYETGLIGAVVLCWLGTYL
                     GRIWKSLRFDLTFAAITVVWLVGATVTTSYNELLSLWLTLGWLTVWPQICEPAPAKAL
                     WQPRPRLARAAREPARTKTALPAGSLAPRRPPAAPRALPPQQRWTDGAEGTSTELS"
     gene            complement(2580..>3425)
                     /locus_tag="VLI90_20475"
     CDS             complement(2580..>3425)
                     /locus_tag="VLI90_20475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV16652.1"
                     /translation="YPKLIPKAVIHWWIRRACREADGLFVHTEALKQQLDRFLGPRAH
                     APIHVVPHGVWTIQHAADHPSLRERLAWKKLLFFGTIRRNKGLDLLLRAAERLPGYSI
                     TIAGELVDRDYFNNEILPLVRRLQAAGVKIDLRDGFTPEDEVAPLFASHSAVILPYTA
                     NFMAQSGVVYLALAHELPVVSSQAGGLGDLFREFKIGETFVQYTPEALATAVEALMAS
                     GSEAQLAEQFRLAKRRYSWQQSAGATIAGYAAAFAERPAADSLPADNQQQTSDDRTVE
                     TTTAQ"
BASE COUNT          645 a         1080 c         1171 g          529 t
ORIGIN      
        1 cgcggccgca acacgatgcg aatcgtcttc gtgcgcagct cctcgtccgg catatcacgg
       61 taaggccggg ccagccggcg gcggttcacc ggaagtgcgg gccattggtt caactcagaa
      121 atttccgagg tgaatcccgc ctcgtcgcaa ttctgaatga tctcggccag ccgcaggcgg
      181 ttggtgtaga agccggactg gcgaatcagg ttcgactccc acatgccggg cgaaaagcgc
      241 aggtgattca gcgacagcgc catcatgtcg cgcaggtcga tgctgtggac gttgcagccg
      301 gctggatgcg tcaagcggcg aagttgcttg agcgtcggca ggaattcatc ccggcggacg
      361 gcctgaagga cggcgttgga gtaggtgaaa tcgatcgatg catccggcaa tcggcgaagg
      421 gccgccagac cgtccgttgc atacgtgagg ccgcatgcgg cggcaacgtc gtccatcgtg
      481 cgggccgagc gcaagtccgg cgcggcaggc aagccgcgat cgcacagata cgccgccatc
      541 ttccgataca gctcgacgtc atcgctggcg aagcgaccga catcgaccag cgacgtcgcc
      601 gatgcgccgt gtgctttggc aacgaccgcg gaaaaaagcg aatcgcccgg ccccagttcc
      661 attgcggtga agccaccgga gtctttgcgg tggaaagccg cggcatcgaa atgccgccgg
      721 aaaacgtcgt gggcgtatgc gggctgggtc atcgccccgt gatgggcgag catcaaccgg
      781 cggcgcacgt ggtacgagat gggaatgcgg ctgaggctga gcttcagcgc gccttttgcc
      841 caccatggca taacggcgcg aatccgatcg cgcgcatggt cggcggtcat cattcctacc
      901 ccagatcgag agatgtgcgt gacccgccga cgtttgacgc cgagagccac cggttcatcg
      961 cgaatgatgg tccaccggcc caacgagcgc cggcggcatg cgcgattaca aatgggtcga
     1021 gcggcagcgc cgcgcgcgcg tacccgcccc tgagcgggtg agcgtgcgat cgacatggcg
     1081 ctgcgatacg tcatcatgat aattcggttg aagttccctc ggcgccgtcg gtccagcgct
     1141 gctgcggtgg cagcgcgcgc ggcgccgcgg gcggccggcg cggcgccaac gagccggcgg
     1201 gcagtgccgt tttagttcgt gctggttctc gcgcggcccg cgccagccgc gggcgcggct
     1261 gccacagcgc tttggccggt gccggttcgc agatctgcgg ccagaccgtc aaccaaccca
     1321 gcgtcagcca aagtgagagc agttcgttgt agctggtcgt gacggtcgcc ccgaccagcc
     1381 agacgacggt gatggcggcg aaggtcaggt cgaagcgcag cgatttccag atgcgcccca
     1441 ggtaagtacc caaccagcac agcaccaccg cgccgatcaa tccggtctcg tagacgtacg
     1501 tgagcagcac gctccagatg gcttccaggt gatagacgcg ctgcaggatc ggcgtggttt
     1561 gaccgggccc gatgccgaag aacagcggga tcagcccgtg atcgacccag agcgagaagc
     1621 cgatcaccag cgaactgctg cgctcttccc acgagctgtt gcccatggcc gatgcgccaa
     1681 agaggcgatc gctgagcgcg atgaaggcga ggtagatgac gatcggcgcg atgacgccaa
     1741 agacgccggc caggaccacg aaggtgcgga aggtcgcccg gcagcgcgtg agccagataa
     1801 tgccaaacgt catcgccgcc gcaagcgtga tggcagagtg accggagcgc gagaggatga
     1861 tcaagcccaa tccccccacc gccgcggcgg cgaagagggc attctgccag gcggcggcgc
     1921 gctggcgaag gcgaatgaca cccgtgaggt gggcgatcca gaaaagcacc cagggcgcca
     1981 gtgaactgga catggcgctg ggctcgggaa agatgccgaa cggccgctgc gtgtagcgcg
     2041 cgatcgtgcg ggcgtactgt tcgacgctga ggaacgacgg gtttgtcgta tacgcccaca
     2101 gcatcgggaa ctcgccgttg tcgaaggcat accactgcca gacaccgaca cccgcgtgaa
     2161 tgatggtggc gacggccatg ccagtcagca tcgccagcgt gtgccgcggg gcgtagagct
     2221 gggcggcgac gatcgtgagc gcgacgacga accagacgag caccgccttg aaggtcagcg
     2281 atagatcgcc gttgccggcc agcgccacct tgaacatgcc gatgaccagc ggcgccatca
     2341 gcagcgggta gaaccacagc gagcgatagc gccacgacag cagcagcgtg ggcacggcca
     2401 tcacgatcgt gagcaggttg ccgaactgga tcgaggagtt gttgccggcg ctgatcgcgg
     2461 catagggcat gagcgcgaag aaccccagcg cggcggcaag ccaggcgccg cggcgctcgc
     2521 ggcgggcgcg cagcgcggct gcggccgcgt ccagcgtggc ctggtgatgc tgcgcgatct
     2581 cactgcgcag tggttgtttc aacggtgcga tcatcgctcg tttgctgttg attgtctgcc
     2641 ggcagcgaat cggctgccgg gcgttctgca aatgccgcgg cgtaaccggc gatcgtcgcg
     2701 ccggccgact gctgccagga ataacgccgc ttggccaggc gaaactgttc tgccagctgc
     2761 gcttcggaac cgctcgccat cagcgcttcg accgcggtgg ccagcgcctc gggcgtgtac
     2821 tgcacgaacg tctcgccgat cttgaactcg cggaacaggt cgcccaatcc accggcttgg
     2881 cttgagacga ccggcagctc gtgcgccagc gcgagataca ccacgccgct ctgggccatg
     2941 aagttggcgg tgtacggcag gatgacggcg ctgtggctgg cgaacagcgg cgccacctcg
     3001 tcttccggcg tgaaaccgtc gcgcagatcg atcttcaccc ccgccgcctg aagccggcgg
     3061 accagcggga gaatctcgtt gttgaagtag tcgcgatcca ccagttcacc ggcgatcgtg
     3121 atgctgtaac cgggcaaccg ctcggccgcg cgcagcagga gatcgagccc tttgttccgg
     3181 cgaatcgtgc cgaagaacag cagcttcttc catgccagcc gctcgcgcag cgacggatga
     3241 tccgcggcgt gttggatcgt ccacacgccg tgcggcacga cgtggatcgg cgcgtgagcg
     3301 cgcggcccga ggaaccggtc cagctgctgc ttcagcgcct cggtgtgaac gaacaagcca
     3361 tcggcctcgc ggcacgcccg gcggatccac cagtgaatga ccgccttggg gatgagcttc
     3421 ggata
//
LOCUS       DATIMH010001189         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4030345, whole genome shotgun sequence.
ACCESSION   DATIMH010001189 DATIMH010000000
VERSION     DATIMH010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4030345"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..294
                     /locus_tag="VLI90_20480"
     CDS             130..294
                     /locus_tag="VLI90_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16653.1"
                     /translation="MKTRIVYRKMSSVRRSPVPRRSSGSSSSLGAFGGMLARLGLASE
                     QLRRARPRSR"
     gene            complement(433..909)
                     /gene="rpsG"
                     /locus_tag="VLI90_20485"
     CDS             complement(433..909)
                     /gene="rpsG"
                     /locus_tag="VLI90_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSV16654.1"
                     /translation="MAYKKFTSSEEMLKPDARFNSKLVSKFINSLMWDGKKTVAQKLF
                     YDAMDQVVKKVKDVPPLELFETAINNIKPYVEVRSKRVGGANYQVPMQVNKRRQQSLA
                     IRWILEACRSQGGRPMSDRLAEELVAAFKKEGKAISTRENTHRMADANKAFAHFAW"
     gene            complement(949..1095)
                     /locus_tag="VLI90_20490"
     CDS             complement(949..1095)
                     /locus_tag="VLI90_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16655.1"
                     /translation="MNTVLSFVAQTGDTSLNDIKHGYASLIFTGCFFLAVIVVTIWWI
                     KRST"
     gene            complement(1193..1564)
                     /gene="rpsL"
                     /locus_tag="VLI90_20495"
     CDS             complement(1193..1564)
                     /gene="rpsL"
                     /locus_tag="VLI90_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSV16656.1"
                     /translation="MPTINQLIKKPRRALGRINKVKDLEASPQKRGVCLQVKTMTPKK
                     PNSALRKIARVRLSNGKEVTAYIPGEEHNLQEHSIVLVRGGRVRDLPGVRYHIVRGTL
                     DALGVDGRKRSRSKYGAKKGK"
     gene            complement(1756..2073)
                     /locus_tag="VLI90_20500"
     CDS             complement(1756..2073)
                     /locus_tag="VLI90_20500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HSV16657.1"
                     /translation="MRKVPYPHPGEILLEEFLKPMGITQYRLAKEIGVSQRRIGEIVA
                     GKRAVTADTGLRLSRFFNLSDGFWTGLQVDFDAAKAKDALATTLGKIKPWSDRSDRRR
                     ASA"
     gene            complement(2087..2368)
                     /locus_tag="VLI90_20505"
     CDS             complement(2087..2368)
                     /locus_tag="VLI90_20505"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSV16658.1"
                     /translation="MPIRSFRCADTQALFAGVRIRCFVAIESVAMRKLAMLNRAGRLD
                     DLRVPPGNRLESLKGNRAGRHSIRINDRFRVCFRWAAGGPVDVEIVDYH"
     gene            2608..>3074
                     /locus_tag="VLI90_20510"
     CDS             2608..>3074
                     /locus_tag="VLI90_20510"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HSV16659.1"
                     /translation="MDTQKILYDAITFDDVLLIPARSEFVPSDADTHTRLTRSIELNI
                     PLLSAPMDTVTEAALAIALAQEGGIGIIHKNLSLENQAREVEKVKRSENGVIVDPITL
                     PPSATIAQARQMMRTYNVSGIPIVDDEAPGGRGQGSEGKIERPQRAKSNGKAP"
BASE COUNT          580 a          945 c          914 g          635 t
ORIGIN      
        1 agacgccggc gtcgggggcc aacttttttt atcggactgt cgcgaaaagt cggaattgtt
       61 ttggatcggc ccgccggctg cgtaggttac tacccgaaca atggccgtct ttggaaagga
      121 gttgccgtta tgaagactcg tattgtgtat cgaaaaatgt cgtccgtccg gcgatcgccg
      181 gtgccgcgtc gctcgtcggg ttcgtcgtcg agcctgggcg cgttcggcgg gatgctggcg
      241 cgactgggct tggccagtga acagctgcgc cgggcgcggc cgcgctcgcg atgaaagcgc
      301 gcacgcgtcg cgctgctcta gttcacaacc cttcccaaaa agaaccgacg gtcggagcag
      361 ttgcgccgac cgtcggttct tttgtttcga ccaaaaagac agagccactg aagtggctcc
      421 gccgtgcgga tgttaccacg cgaagtgcgc gaaggccttg ttggcgtcgg ccatgcggtg
      481 cgtgttttca cgcgtggaga tggccttgcc ttctttcttg aacgcggcca cgagctcttc
      541 ggcgaggcgg tcggacatcg ggcggccgcc ctggctgcgg caggcctcga ggatccagcg
      601 gatggccagc gactgctggc ggcgcttgtt gacctgcatc gggacctggt agttggcgcc
      661 gccgacgcgc ttggagcgca cttcgacgta cggcttgatg ttgttgatgg cggtctcgaa
      721 cagttccagc gggggaacgt ccttgacctt cttcacgact tgatccatcg cgtcgtagaa
      781 gagcttctgc gcgacggtct tcttgccgtc ccacatcagg ctgttgatga atttgctcac
      841 cagcttgctg ttgaagcgtg catccggctt caacatctct tcgctggacg tgaatttctt
      901 ataagccata gtcgttcgtc gtccgttttc agttctacgt cagtcctgtc aggtggatcg
      961 cttgatccac cagatcgtca ccacaatcac cgcgaggaaa aaacagccgg tgaaaatcaa
     1021 gctggcgtag ccatgcttga tgtcgttaag gctcgtatcg cccgtctgcg ccacgaagct
     1081 caacacggta ttcacttgtc ttctctcctg ctgcgccgca cgggccaggc gttacggatc
     1141 acccacgcgc gacgcggtgc gactcgggca caagcaggag ctgagtcagc tcttacttgc
     1201 ccttcttagc gccatacttg ctacggctgc gtttgcggcc gtcgacaccc agcgcatcga
     1261 gcgtgccgcg gacgatatga taccgcacac ccggcagatc gcgcacgcga ccgccgcgga
     1321 ccagcacgat gctgtgctcc tgcaggttgt gttcttcgcc ggggatatag gcggtcactt
     1381 ctttgccgtt gctgaggcgc acgcgggcga tcttgcgaag ggccgagttc ggcttcttcg
     1441 gcgtcatcgt cttgacctgc aggcacacgc cacgcttttg cgggctggct tccagatcct
     1501 tgaccttgtt gatgcggcca agggcgcggc gaggcttctt aatgagctga ttgatcgtcg
     1561 gcatacggtt cctaaaggcg ttcgtaactc gtgcaattgt ctcaggttcc accatttcga
     1621 gagatgcgca accgcgcagc cccggaatgg ggaaagtccg ggaatatact ggcgttagcg
     1681 cggcatggca agacgtcggg tcaaaactag gtgtggggct ttggtgtacc tgaggaagcg
     1741 acgttccgcg acacactaag cagacgcgcg acgccggtca ctccgatcgc tccaaggttt
     1801 gatcttcccg agcgtcgtcg ccagcgcatc tttcgccttg gctgcgtcga aatccacctg
     1861 cagtccggtc cagaagccat cggaaagatt aaagaaccgc gagaggcgaa gccccgtgtc
     1921 ggcggtgacg gcgcgcttgc ccgcgacgat ctcaccgatg cgccgctgcg acacgccgat
     1981 ctccttcgcc agccggtact gcgtgatgcc catcggcttg aggaattcct caagaaggat
     2041 ttcacccggg tgaggatatg gaacttttcg catcgtgctc acgtgctcag tggtaatcga
     2101 caatctcgac atcaacgggt ccgccggcag cccatctgaa gcagacgcga aaccgatcat
     2161 tgatgcgaat gctgtgccgc cccgcgcggt tgcccttgag cgattccagc cgattgcccg
     2221 gcggcacgcg aagatcgtca agtcttcctg ctcggttcag catcgccagc ttccgcatgg
     2281 ccaccgattc aatcgcgacg aagcacctga ttcgtacacc tgcaaacaac gcctgtgtgt
     2341 cggcgcatct gaacgagcgg atcggcaccc gcgtatacta acgcgccgcg ttagtaggtt
     2401 caatgagcca ttcaacgcct actttattct gacataccgg tctccgaagc gcgatttcac
     2461 cattcccgtt ggcttccgaa gtaaagccca cgattctgcc gtgggaccct tcccttccaa
     2521 aacgtcccac ggcatagccg tgggcttttg gcgccggact tcgaagctca gcttgacacc
     2581 cgcgcgctgc ccccttaaca gtatcccatg gatacccaga agatcctcta cgacgccatc
     2641 acgttcgacg atgtgctgct gattcccgcc cgcagcgaat tcgttccgtc cgacgccgac
     2701 acccacacgc gccttacccg cagcatcgag ctcaacatcc cgctgctctc ggcgcccatg
     2761 gacaccgtga ccgaggccgc cctggcgatc gccctggcgc aggaaggcgg catcggcatc
     2821 attcacaaaa atctgtcgct ggaaaaccag gcgcgcgaag tggagaaggt gaagcgcagc
     2881 gagaacggcg tcatcgtcga tccgatcacg ctgccgccga gcgcaacgat cgcacaggcc
     2941 cggcaaatga tgcggacgta caacgtcagc gggattccca ttgtcgatga cgaggcgccg
     3001 gggggcaggg gtcaggggtc agaggggaag atcgagcggc cgcagcgcgc caagagcaat
     3061 ggcaaggctc ccaa
//
LOCUS       DATIMH010001190         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3457080, whole genome shotgun sequence.
ACCESSION   DATIMH010001190 DATIMH010000000
VERSION     DATIMH010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3457080"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VLI90_20515"
     CDS             <1..835
                     /locus_tag="VLI90_20515"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSV16660.1"
                     /translation="PRLWSKPIHDAVEEANRLVAAGHVELVLTGIFLGAYGQATALRR
                     RQSEGDAPIAKLIDALCTRVPGLRRLRLSSLEPGDLTADLLAALRSHEQVVPHFHLPL
                     QSGSDTILHRMNRQYTRDDYLRMLDDVRDAFDRPALTTDIIVGFPGETAADFDRTLEV
                     VEQARFIHIHAFSFSPRPGTAAARWSGDFVRGPIVNERIDELNRRAQLHSHVFRQSFV
                     GEEVELLVERPGAEAGNLRHGRCQRYFSVFFDSEMAREGDFVRVRIDRATPHRTLGTL
                     R"
     gene            832..1284
                     /gene="dtd"
                     /locus_tag="VLI90_20520"
     CDS             832..1284
                     /gene="dtd"
                     /locus_tag="VLI90_20520"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HSV16661.1"
                     /translation="MIAVIQRVTQASVTVNTRTVGAIERGILALVAVHKTDTSKDVEW
                     MAKKLTALRIFPNREKNFDQDVLTIAAGILLVSNFTVAAATAQGRRPSLDAAADPVKG
                     RELFDQLIKTVRATGVTTATGEFGGDMRVSLQNDGPVTFIVDSRPSPA"
     gene            complement(1489..>2042)
                     /locus_tag="VLI90_20525"
     CDS             complement(1489..>2042)
                     /locus_tag="VLI90_20525"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSV16662.1"
                     /translation="LDEAQRYGRLAERAGTRLLRIFGGGLPKGMSREEGLIMAQRHLR
                     QIVKICKAYGCVPILETHDDWATSTAVLELLHEFDPADVGVLWDVEHPWRRGESPADT
                     ARALRKFLRHVHVKDSRGDERLPTLLGEGSLPLADAAAALRGIEYDGWYCLECEKRWD
                     EKAPEPEVSLPQFATYMRERWNR"
BASE COUNT          359 a          729 c          598 g          356 t
ORIGIN      
        1 tccaagactg tggagcaagc cgatccacga cgcggtggag gaagcgaatc gcctcgtcgc
       61 cgccggccat gtcgagttgg tgctgaccgg catcttcctc ggcgcgtatg gccaagcgac
      121 agcgctgcga cggcgacaga gtgaaggtga cgcgccgatt gccaagctga tcgacgcgct
      181 ctgcacgcgc gtgccgggcc ttcgccgctt gcggcttagc agccttgaac ccggcgacct
      241 gacggccgat ctcctcgccg cgttgaggtc gcacgagcaa gtggttccgc acttccacct
      301 cccgctccag agcggctccg acacgatcct gcaccgcatg aaccgccagt acacgcgcga
      361 tgattacctg cgcatgctcg acgatgtgcg agacgccttc gatcgccccg cgctcacgac
      421 agacatcatc gtcggcttcc ccggtgaaac cgccgccgac ttcgatcgca cgcttgaagt
      481 ggtcgagcaa gcccgcttca tccacatcca cgcgttttcg ttctcgccaa gaccgggaac
      541 tgccgccgcg cgctggtctg gcgactttgt gcgcgggccg atcgtcaatg agcgaatcga
      601 tgaactgaac cgccgcgcgc agctgcacag ccacgtcttc cgccaatcgt tcgtcggcga
      661 agaagtcgag ctgctggtcg agcgccccgg cgccgaagcc ggcaacctcc gccacggccg
      721 ctgccagcgc tacttcagcg tcttcttcga cagcgagatg gcgcgcgagg gagattttgt
      781 tcgcgtgcgg atcgaccgtg ccacgccaca tcgcacactg gggaccttaa gatgatcgcc
      841 gtcatccagc gcgtcaccca ggcctccgtc accgtcaaca cgcgcaccgt cggcgccatc
      901 gaacgcggca tccttgcgct cgtcgccgtt cacaaaaccg acacgtctaa agatgtagag
      961 tggatggcaa aaaagctgac cgccctgcgc atcttcccca accgcgagaa gaacttcgac
     1021 caggatgtgc tgacgatcgc cgccggcatt ctgctcgtca gcaatttcac cgtcgccgcc
     1081 gcgaccgccc agggccgccg gccgtcgctc gacgccgccg ctgacccagt caaagggcgt
     1141 gagttgttcg atcaactcat taagaccgtc cgcgccaccg gcgtcacaac ggccaccggc
     1201 gagttcggcg gggacatgcg ggtgtcactt cagaacgatg ggccggtcac cttcatcgtc
     1261 gacagccgcc cctctcccgc gtagcggtac cgtagcacgg gcgtctcgcc cgtggtgcgt
     1321 gtaatcatgc acggtcgatg tacctatcga cacagcaatc gcgtcagcga cagttggcga
     1381 aatcccgtcg cttgcgcgat tgctttatcc acggagtacc gtcgatattc gtggagtacc
     1441 acgaaacacg ggcaagatgc ccgtgctacg gtactgctac gcaggaagtt accggttcca
     1501 ccgctcgcgc atatacgtcg cgaactgcgg caagctcact tccggctccg gcgctttctc
     1561 gtcccaccgc ttctcgcact ccagacaata ccagccgtcg tattcgattc cgcgcagcgc
     1621 cgccgccgcg tccgccagcg gcaggctgcc ctcgcccagc agcgtgggca ggcgctcgtc
     1681 gccgcggctg tccttgacgt gaacgtgtcg caggaatttc cgcagcgccc gcgccgtgtc
     1741 cgccggcgat tcaccgcggc gccagggatg ttcaacatcc caaagcacgc ccacgtcggc
     1801 cgggtcgaac tcgtgcagca gctccagcac cgcggtgctg gttgcccagt catcgtgcgt
     1861 ttccaggatc ggcacgcagc cgtacgcctt gcagatcttc acgatctgcc gaaggtgccg
     1921 ctgggccatg atcaggcctt cctcgcggct catccccttg ggcaagccgc cgccgaagat
     1981 gcgcagcaac cgcgtgccgg cgcgctcggc gagcctgccg tagcgctgcg cttcatccag
     2041 ca
//
LOCUS       DATIMH010001191         1736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_572971, whole genome shotgun sequence.
ACCESSION   DATIMH010001191 DATIMH010000000
VERSION     DATIMH010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572971"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..419)
                     /locus_tag="VLI90_20530"
     CDS             complement(78..419)
                     /locus_tag="VLI90_20530"
                     /inference="COORDINATES: protein motif:HMM:NF016245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HSV16663.1"
                     /translation="MGARVLPVLLLVGSNIFMTFAWYGHLKFKEVALWKVILVSWLIA
                     SLEYCLQVPANRMGSRVYTPDQLKVIQEVITLSVFGVFSVYYFGDKLHWNHAVAFGCL
                     VTGAFFMFHKF"
     gene            458..1168
                     /locus_tag="VLI90_20535"
     CDS             458..1168
                     /locus_tag="VLI90_20535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HSV16664.1"
                     /translation="MPVAQNLARIREQIAEACRRSNRSESEIALMAVSKMHPAEAVIE
                     AYAAGLRLFGENRVQEWQEKRPAIQHLTDAKCHLIGPLQSNKTAKAAELFDAVDSVDS
                     LRIATRLDAAARAVDKRLPILIEVKLSHEESKHGIGPAELPALLVALAPLESLVPSGL
                     MTVPPWSEEAEPARPYFRELRRLRDASLVQYPTLTQLSMGMSNDFTVAIDEGSTCIRI
                     GTALFGKRAYPAKAAASE"
     gene            1165..1461
                     /locus_tag="VLI90_20540"
     CDS             1165..1461
                     /locus_tag="VLI90_20540"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HSV16665.1"
                     /translation="MIALRDTPDGCTLPVRVHPGAKRNAVTGTHDGAMKISLTTPPTD
                     GRANEALIAFVAERLNLPRSRIALVSGQTSRSKVLRITGLNAAEVESRLGPDLC"
     gene            complement(1475..>1736)
                     /locus_tag="VLI90_20545"
     CDS             complement(1475..>1736)
                     /locus_tag="VLI90_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16666.1"
                     /translation="LGRLTPKFHGITIENVTATNSGSDGTIVGLPESPVTDLTLKNVH
                     LSGKTGMSIAYATVKMDDVTFKADQGENLKVASTAKVTGYKQ"
BASE COUNT          383 a          576 c          471 g          306 t
ORIGIN      
        1 agtacagcag agcgatggcg ccaactctct gattttattc cagttacacg caaaatcagt
       61 gggggggggg gagggggtca gaacttatgg aacatgaaga aggcgcccgt cacgaggcag
      121 ccgaatgcca cagcgtgatt ccaatgcagc ttgtcgccga agtagtagac cgaaaaaacg
      181 ccaaacacgc tcagcgtaat cacctcctga atcactttca gttgatcggg ggtataaacc
      241 ctcgaaccca tgcgattcgc aggaacctgc aggcagtact ccaatgaggc gatcagccaa
      301 ctcaccagga tcaccttcca aagagcgact tctttgaact tcaagtgccc ataccaggca
      361 aacgtcatga atatgttcga acccaccagc aacagcacag gaagcacacg cgcgcccatc
      421 caatttcctc ccggaaaact cgtaagatgc ttctagaatg ccagttgccc aaaacctcgc
      481 ccgcatccgc gagcagatcg ccgaagcttg ccgccgcagt aaccgctccg agtccgagat
      541 cgcactcatg gcggtcagca aaatgcaccc cgccgaagcc gtgatcgaag cctacgccgc
      601 cggcctgcgc ctctttggcg agaaccgcgt ccaggaatgg caggaaaagc gccccgccat
      661 ccagcacctc accgacgcga aatgtcacct catcggcccg ctgcagtcga acaaaaccgc
      721 aaaggctgcc gaacttttcg atgccgtcga ttccgtagac tcgctcagga ttgcaacccg
      781 cctcgacgcg gctgcgcgag cggtcgacaa aaggctgccg atcttgatcg aagtgaaact
      841 gagccacgag gagtcgaagc acggcatcgg gcccgccgaa ctccccgcgc tgctcgtcgc
      901 gctcgccccg ctcgaatcac tggttcccag cggcctgatg accgttccgc cgtggtcgga
      961 agaagccgaa cccgcacggc cctatttccg cgaacttcgc cgcctgcgcg atgcgtcgct
     1021 cgtacagtac ccgacgctca cccagctctc gatgggcatg tccaacgact tcaccgtcgc
     1081 gatcgacgaa ggcagcacct gcatccgcat cggcaccgcg ctcttcggaa aacgggctta
     1141 cccggcgaaa gctgcggcca gcgaatgatc gcgctgcggg acacgccaga cggctgcacg
     1201 cttccagtgc gcgttcatcc tggggcgaaa cgcaacgcag tgaccggcac gcacgatggc
     1261 gcgatgaaga tatcgctcac gacgccgccg actgatggtc gcgctaacga agcgctgatc
     1321 gcgttcgtgg ccgagcgcct caacctccca cgctcgcgca tcgccctcgt gagcggccag
     1381 acgagccgca gcaaggtcct gcgcattacc ggcttaaacg ccgcagaggt cgaatcccgg
     1441 ttgggacccg acctctgctg accatctgct gcgcttattg cttgtagcca gtaaccttcg
     1501 ccgtcgacgc gaccttcagg ttctcgccct gatcagcctt gaaggtgaca tcgtccatct
     1561 tgacagtcgc ataagcgatc gacatgccgg tcttgccgga caggtggaca ttcttcagcg
     1621 taaggtccgt caccggcgat tctggcagtc cgacgatcgt cccgtctgat ccgctgttgg
     1681 tcgccgttac gttctcaatc gtgatgccgt ggaacttcgg cgttagacgg ccaagc
//
LOCUS       DATIMH010001192         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1441425, whole genome shotgun sequence.
ACCESSION   DATIMH010001192 DATIMH010000000
VERSION     DATIMH010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441425"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VLI90_20550"
     CDS             <1..427
                     /locus_tag="VLI90_20550"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSV16667.1"
                     /translation="HATRAVLPDMKRRGWGRIVNVASAHGLIGSAFKGAYVAAKHGVV
                     GFTKVTALEAAESGITCNAVCPGYVWTPLVEQQVAAQANAHGLSPEEVVRKVFLAEQP
                     TRKFATVSEIAGTVAFLCGPASASITGTTIAVDGGWTAH"
     gene            640..1137
                     /locus_tag="VLI90_20555"
     CDS             640..1137
                     /locus_tag="VLI90_20555"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSV16668.1"
                     /translation="MTNLTQKLRSTHRLLATAAVLTCFAAGAAFAGGCPTDKMVAEGK
                     GQAMSSQPAAGVTDDVIATNDLAKQPVNINDRLFRLRRLVIQPGGVVPWHSHGDRPAI
                     IYIVSGEIIEYASTCAVPIVHKAGEATAEKSPTQHWWKNTGSVPVELLSADLFHKQKT
                     DEHMM"
     gene            1249..2442
                     /locus_tag="VLI90_20560"
     CDS             1249..2442
                     /locus_tag="VLI90_20560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSV16669.1"
                     /translation="MTSYRHDALHFVVIATTAFLTLVDLFAAQAILPFLAAAYKVSAA
                     AMSFAVNASTIGMAAGGLGVALFSARIDRRLGILCALGVLAVPTLLLSLLPALPIFTI
                     LRIVQGLCMSSAFALTLAYLGEHESPSRAAVAFAAYVTGNVASNLVGRMISAGVADHF
                     GLAANFQVFALLNVAGAVLVWFTIEATPRAITRSMTGMRAAGSWLAQLRDQRLLAAFG
                     IGFCLLFAFIGTFTFVNFVLVGPPLDLGMMQVGFVYLVFLPAILTTPLAGSFVARFGT
                     RPALWGALAVALLGLPFLVSPHLRAVLTGMVLVSAGTFFAQAIATGFVSRTAETDRAA
                     ASGVYLASYFAGGLAGSVVLGLLFDRAGWSACVMGIAGALLLACALTIRLRAAASRPA
                     IASLT"
BASE COUNT          388 a          879 c          800 g          493 t
ORIGIN      
        1 ccacgccacg cgcgcggtgc tgccggacat gaaacgccgc ggctgggggc gtatcgtcaa
       61 cgtcgcctcg gcgcacggac tgatcggctc tgcgttcaag ggcgcttatg tcgcggccaa
      121 gcacggcgtg gtcggcttca ccaaggtgac cgcgctggaa gccgccgaat cgggcatcac
      181 ctgcaacgcg gtatgtccgg gatatgtgtg gacgccgctg gtggaacagc aggtcgccgc
      241 gcaggcgaac gcacacggac tgtcgccgga ggaggttgtc cgcaaggtgt tcctggccga
      301 acagccgacg cggaaattcg ccacggtctc ggaaatcgcc ggcaccgtcg cgttcctgtg
      361 cgggcccgcg tctgcgtcga tcaccggcac gacaatcgcg gtcgacggcg gctggacggc
      421 gcactgaaag cgccaggcgg aattttgtta tcgtctcgtg acaggccggc cggcgatctc
      481 cggccggcct gttccgcgtc cgcgcgcggg ccgacccgaa actatcataa ccatcgtgca
      541 tccattccag aacatatcgg tattttatca atcaaatcgc cttcgtcatt atccttctca
      601 ggcacggcgc tccggccggc cgagacacag gagaacgaaa tgaccaatct cacccagaag
      661 ctccgttcca cgcatcgcct gctcgccacc gccgccgtat tgacctgctt cgccgcgggc
      721 gccgctttcg ccggtggctg cccgaccgac aagatggtcg cggaggggaa gggccaggcg
      781 atgtccagcc agccggccgc cggcgtcacc gacgacgtga tcgccaccaa cgatctcgcg
      841 aaacagcccg tcaacatcaa cgaccgtttg ttccgcttgc gccgcctggt gatccagccc
      901 ggaggcgtcg tgccgtggca cagccatggc gaccggcccg cgatcatcta catcgtctcg
      961 ggtgaaatta tcgaatatgc cagcacctgc gccgtgccga tcgtccacaa ggccggcgag
     1021 gccaccgccg agaagtcgcc cacgcaacac tggtggaaga acaccggctc ggtgcccgtg
     1081 gaactgctgt cggccgacct gttccacaag caaaagaccg atgagcacat gatgtaacga
     1141 ccacgcgtca tgccatcgga cgacgcccgg cgatcacgcc tccgcaccca ttctccctcg
     1201 tgagcgcaga tcctgggcgt cgtccgcccc tcgcaccgga gtaccgaaat gacttcgtat
     1261 cgacacgacg cgctgcattt cgtggtgatc gccacgacgg cgtttctcac tctggtggat
     1321 ctgttcgccg cccaggcgat cctgccgttc ctcgccgccg cctataaggt cagcgcggcg
     1381 gcgatgagtt tcgcggtcaa cgccagcacg atcggcatgg cggccggcgg cctcggtgtc
     1441 gccctgttct ccgcccgtat cgatcgccga ctgggtattc tatgcgcgct tggcgtgctc
     1501 gcggtgccca ccttattgct cagcctgtta cccgcgctgc cgattttcac tatcctgcgg
     1561 atcgttcagg ggctgtgcat gtcctccgct ttcgcgctga ccctggcgta tctgggggag
     1621 catgaaagcc cgtcgcgtgc cgccgtcgcc ttcgcggcct atgtcaccgg caatgtcgcc
     1681 agcaatctgg tcggccggat gatttccgcg ggcgtcgccg atcatttcgg gctcgcggca
     1741 aattttcagg tattcgcgct gctcaatgtc gccggcgcgg tcctggtctg gttcaccatc
     1801 gaggcgacgc cgcgcgcgat cacgcgaagc atgaccggca tgcgcgccgc cggctcatgg
     1861 ctggcccaat tacgcgatca gcggctgctg gcggcgttcg gcatcggctt ctgtttgctg
     1921 ttcgccttca tcggcacctt caccttcgtc aatttcgtgc ttgtcgggcc gccgctcgat
     1981 ctgggcatga tgcaggtggg gttcgtttat ctcgtctttc tgccggcgat cctgaccacg
     2041 ccgctggccg gttcgttcgt ggcacgtttc ggcacccgac ccgccctgtg gggcgccctg
     2101 gccgtcgcgc tgctcggcct gccgttcctg gtttcgccgc atctgcgtgc cgtgctgacc
     2161 ggcatggtgc tggtttccgc cggcacgttc ttcgctcagg cgatcgcgac cgggtttgtc
     2221 agccggacgg cggagacgga tcgggccgcc gcgagcgggg tgtatctcgc gagttatttc
     2281 gcgggcggac tggccggcag cgtcgtgctc ggcctgctgt tcgatcgtgc cggttggtcc
     2341 gcctgcgtga tgggcatcgc cggtgccctg ctgctggcct gcgccctgac cattcgcctg
     2401 cgcgccgccg cgtcccggcc ggcgatcgcg tccctgacct gaccgataca aggaggatcc
     2461 aaccatgaac gccatcacac cagccgaggt cgtcgccaac cacacggtgt ccaaaggcac
     2521 gccgccgcga gtcattttgg ttctgcaagg cggcggcgca
//
LOCUS       DATIMH010001193         6074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3749471, whole genome shotgun sequence.
ACCESSION   DATIMH010001193 DATIMH010000000
VERSION     DATIMH010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3749471"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..884)
                     /locus_tag="VLI90_20565"
     CDS             complement(159..884)
                     /locus_tag="VLI90_20565"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HSV16670.1"
                     /translation="MSTRYVPKTIHTANMPYREPALPEAPPVQPFVTIAQQAGAESPA
                     LARRLTEMLSAREPRSSASSSSSPAAWSHWDQELIEKVSADSQIPADLISSLETSGHS
                     WIDDLLAGVFQRPDDLVIFHRVKDCIRDLARAGHVVLVGHGSVFMTCDLPGGVHIRLI
                     APLRVRARNVAARFGLSYDQALRKMRRIDRQRRTFFRRFWPNQPLSPQIFSAVLNTAL
                     LDEDRLLRAIAAMLPPPPQASGR"
     gene            complement(937..1590)
                     /locus_tag="VLI90_20570"
     CDS             complement(937..1590)
                     /locus_tag="VLI90_20570"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HSV16671.1"
                     /translation="MTNRQPDLSSLAQKLEHELELLEGSLRRLRLSWLLRHFPPRQIW
                     AGFVCVNSFVAIALLSTVAMVSRSPFVFPSLGPTAYLLFFTPRAAAASPKHAICGHAI
                     GLACGWAALWFSGLYHAPAVTAEGVNLTRVFAAAGSLGATGALMVLCNVGHPPAGATT
                     LIVSLGFITRIEYLLIIEAAVLALVLHALVMNRLSGIDYPLWSPRRHWAPLPRPPVD"
     gene            complement(1729..2490)
                     /locus_tag="VLI90_20575"
     CDS             complement(1729..2490)
                     /locus_tag="VLI90_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16672.1"
                     /translation="MRWTLIQLTSFRRLWAAERLVDEDLQALEAAIMRDPAGPPVMRG
                     TGGLRKNSICATVARQRQEQLHACRIRPISRLQANLPGHALLEEKHRKPLGCRRARHS
                     FIVVSIGTRAARRAKSMSAISRRKGVSRIGRKRAARQSKAARTTAKGREIVASLREAI
                     EVEESRLPVESRFTVRTVELPRPAMKYDAVTVRATRDRLGVSQTIFAQLLGVSPMLVR
                     AWEQTQRSPAMWARRLLDEVNRDPRHWQRMLRRAS"
     gene            2811..4241
                     /locus_tag="VLI90_20580"
     CDS             2811..4241
                     /locus_tag="VLI90_20580"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSV16673.1"
                     /translation="MDGMSIGAGASAPNGIHTDFNGWLQQHAGRAYELHERHINPVFV
                     KMLRTIGFDKGYVRGEGCYLWDAAGTKYLDLLTGWGVFALGRNHPKVKAIIQQLMNEN
                     HPNLVRMDCSLLSGLVAEKLTHHAGGDLSRVFFCNSGTESIETAIKFARCATGRQNIV
                     YCDHAFHGLTTGSLSLNGADFFRERFGELMPGTSKVPFNDLPALEKALSSKTVAAFIV
                     EPVQGKSCEVVADGYLADVQRLCNKFGTLFICDEVQCGLGRTGKWFAYQHWANVEPDI
                     VCVAKALSGGYVPVGAVLTRPKIMDCVFNSMERCVVHSNTFGQNDMAMAAALASLHVI
                     EEDKLVENAAAMGEYAMTRLREIGKDCPFVSAIRGKGLMFGIDFDRPKESFKLKMAWD
                     MLHKMNFGVFGQMVIIPLLQKHHILTQVAGYHTEVIKFLPPMTITKADMDWFLGAMQE
                     TLMDTTRFPGAAWETVFGLAKRAMMA"
     gene            4367..4942
                     /locus_tag="VLI90_20585"
     CDS             4367..4942
                     /locus_tag="VLI90_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16674.1"
                     /translation="MEARRSRWRTLASVRLIFIGVLALAAGMFWRFGEALLAPAEASE
                     QQLVYRTNHAKILEACRWIEAKRATLGLKGETIDNVGAVHDALPDVVRQLDLELIRVE
                     DTGVMIYFGGGFGHWGFEAPHTDPTPQDDRGPWQLIPGRWYWNEWGRFPRDPAKPVRV
                     RTSTLILAGGLMLIAAGIGGLRTGQPRQPGA"
     gene            4947..5153
                     /locus_tag="VLI90_20590"
     CDS             4947..5153
                     /locus_tag="VLI90_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16675.1"
                     /translation="MDTTSLMLSVLFGAVGMGFLVYGKKAGRLIPIVAGLALMIIPYM
                     ISNNYVLTGVCAGLTAWPFVMRDL"
     gene            complement(5154..>6074)
                     /locus_tag="VLI90_20595"
     CDS             complement(5154..>6074)
                     /locus_tag="VLI90_20595"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSV16676.1"
                     /translation="VPLMLGPSPAAFLYLDARANRPGHAHMVAPMRPHAAAFCGALGR
                     LAGLALANLKRVEIERRQAMIEHDLNAAAAAQRWVLPPRHTSIGPFTITGESRPGRFV
                     GGDFFDVIDLGNDRIAVALGDVSGKGVEASVLMTATQGFLHAALQLHGEPARAVTAAN
                     AFVAPRRPDCRFVTLWVALLDLPSRTLTYVDAGHSYATLFDDAGAMTELNLGDGPPLG
                     VADDYTYKIETIPLPPHGRLAIVSDGFIEQPAPAAGDDGQRIQFEMAGVCRCLSATNE
                     DDVAALFSAIVAHAGSNQLADDATAVCVRW"
BASE COUNT         1154 a         1961 c         1896 g         1063 t
ORIGIN      
        1 ttcgatagcg tctggatatc cgaaaggtcc gcgcgggcta aagccccgcg gctgcgacga
       61 gtcatttctg ctcccgcgag gtgtgccgga gcccggtgcg gtggtcgcgc gcggtgcagc
      121 atcttcgccc atgaacatag atgcgcctgc gccatgagct atcgaccgct cgcctgcggt
      181 ggcggcggta gcatcgcggc gatcgcgcgc agcaggcgat cttcgtctaa caacgccgtg
      241 ttcagcaccg ccgagaaaat ctgcggcgac agcggctgat tcggccagaa gcgccgaaag
      301 aacgtccgcc gctggcgatc gatccgccgc atcttccgca aggcctggtc gtacgacaga
      361 ccaaagcgcg cggcgacgtt tcgggcgcgc acccgcagcg gcgcgatgag ccggatgtgc
      421 accccgcccg gcagatcgca cgtcatgaac accgacccgt ggccgaccag gaccacgtgc
      481 cccgcgcggg cgaggtcgcg aatgcaatcc ttgacgcgat ggaagatgac caggtcgtcc
      541 ggccgctgga acacacccgc cagcaggtcg tcaatccacg aatggcccga tgtttccagc
      601 gaagaaatca aatcggccgg aatctggctg tcggccgaca ccttctcgat caattcctga
      661 tcccaatgcg accacgccgc aggcgatgat gatgatgacg ccgaagatcg cggttcgcgc
      721 gcgctcagca tttccgtcaa ccgccgcgct agcgccggcg attccgcgcc cgcttgctgg
      781 gcaatggtga cgaacggctg caccggcggc gcctcaggca acgccggctc gcggtaaggc
      841 atgttcgcgg tgtgaatcgt cttcggcacg tatcgagtgg acatggcaat cgaatcatat
      901 gcgcgcgtcg tcccaatcat cgagcaaatc gaatgatcaa tctaccggcg gccggggcag
      961 tggcgcccaa tgccgtcgcg gcgaccacag cgggtagtcg atccccgata gccggttcat
     1021 caccaacgca tgcagcacca gcgccaagac ggcggcctcg atgatcaaca agtattcgat
     1081 cctggtgatg aacccgagcg acacgatgag cgtggttgcc cccgccgggg gatggcccac
     1141 gttgcacagc accattagcg cgccagtcgc gcccagcgaa ccggccgcgg caaatacccg
     1201 cgtcaggttc acgccttcgg ccgtcaccgc cggcgcatga tacaggccgc tgaaccacag
     1261 cgcggcccat ccgcatgcca gcccgatcgc gtgaccgcag atcgcgtgct tgggactggc
     1321 cgcggccgcg cgcggcgtga agaacagcag atatgccgtc ggccccagcg acggaaacac
     1381 aaacggcgaa cgcgacacca ttgccaccgt cgacagcagc gcgatggcca cgaaactatt
     1441 cacgcaaacg aaccccgccc aaatctgccg cggcggaaaa tggcgcagca accagctcaa
     1501 gcgcagccgc cgcagggagc cttcgagcag ctccagctcg tgctcgagct tctgggcgag
     1561 cgaagataga tcgggttgac ggttggtcac gtctgaagtc gcgcgcgacg agcggagccg
     1621 tgaccatcgt gcgaatcacg gcaagcgccg ccgaatgggg acgagcaact ctcaagccgc
     1681 aaagctggcg cggccagttg gccacgttca ttcgagttgc ggcttaagtt acgatgcccg
     1741 acgaagcatt ctctgccaat ggcgcggatc gcgattgacc tcatcgagca gccggcgcgc
     1801 ccacatggcc ggcgagcgct gggtctgttc ccaggcccgc accagcatcg gcgacacgcc
     1861 cagcagttgc gcgaagattg tttggctcac acccagccga tcgcgcgttg cgcgcacggt
     1921 gaccgcgtca tatttcatcg ccggccgtgg tagttcaacc gtccgcacgg tgaaccgcga
     1981 ttcaaccggc agccgcgact cttccacttc gatcgcctca cgcagcgaag cgacgatttc
     2041 gcggcctttc gccgtcgtcc gcgcggcctt gctctgtcga gccgctcgtt tgcggccgat
     2101 tcggcttaca ccttttcggc gtgagatagc gctcatggat tttgcccttc gtgcagcgcg
     2161 cgttccaatt gagacaacaa tgaatgaatg tcgcgcccgt ctgcagccga gaggttttcg
     2221 gtgcttttct tcaagaagag cgtgaccagg taaattcgcc tgaagtcggg aaattggacg
     2281 aatgcgacac gcatggagct gctcttgcct ttgccgcgcg acggtggcgc aaatcgaatt
     2341 tttgcgcagt ccgccggtcc cccgcatgac aggcggccca gccgggtcgc gcatgatggc
     2401 ggcctccagc gcctgtagat cctcgtccac caatcgctca gcagcccata accgccggaa
     2461 gctcgtgagc tgaatgagcg tccatcgcat tctcgctcag cataatactc ggtattacta
     2521 tcgcaacatt tgccgggtat gaattcctac ttcctttagg caaccacgac gcgcttcggc
     2581 ggttcccgcc aacttcccca tcgtcggcgg gtgtaactga actttgtggt ccggccgttc
     2641 tgtgatcccc tcgcccggta ctgctgaccg caggtctccg ggagaggggg actgacccga
     2701 atcgagccac atattaaagt cataccctcg gcgatcgtcg gcttttcatc ccccgccccg
     2761 gtcgcggtcc tcatcccaat cgctataatc ctccgatctt ggagaacatg atggacggca
     2821 tgagtatcgg cgccggggct tcggccccga acggaatcca tacagatttc aacggctggc
     2881 tgcagcagca cgcggggcgg gcgtatgagc tgcacgagcg gcacatcaac cccgtcttcg
     2941 tcaaaatgct gcggacgatc ggcttcgaca agggctacgt ccgcggggaa ggctgctacc
     3001 tgtgggacgc ggccggcacg aaatacctcg acctgctgac cggttggggt gtcttcgcgc
     3061 tggggcgaaa tcacccgaag gtgaaggcga tcatccagca actcatgaac gagaaccatc
     3121 cgaacctggt gcggatggat tgcagcctgc tgagcgggtt ggtcgcggaa aaactcacgc
     3181 accacgcggg cggcgatctg tcgcgcgtct tcttctgcaa cagcggcacc gagtcgatcg
     3241 aaaccgccat caagttcgcc cgctgcgcca ccggccgaca gaacatcgtc tattgcgatc
     3301 acgcatttca tgggctgacg acgggttcac tttcgctcaa cggcgcggac ttcttccgcg
     3361 aacgatttgg cgaactgatg ccgggcacca gcaaggtgcc gttcaacgat ctgccggcgc
     3421 tggagaaggc gctgagcagc aagacggtgg cggcgttcat cgtcgaaccg gtgcaaggca
     3481 agagctgcga agtggtcgcc gacggttatc tcgccgacgt ccagcgcctg tgcaacaagt
     3541 tcggcacgct gttcatctgc gatgaagtcc aatgcggcct tggccgcacg ggcaagtggt
     3601 tcgcgtatca acactgggca aacgtcgagc cggacatcgt ctgcgtcgcc aaggcattgt
     3661 ccggcggata cgtccccgtc ggcgctgttc tcacccggcc caagatcatg gactgcgtct
     3721 tcaattcgat ggagcgctgc gtcgtccaca gcaacacgtt cggccagaac gacatggcaa
     3781 tggccgcggc gctggcgagc ctgcatgtga tcgaggaaga caagctggtg gagaacgccg
     3841 ccgcgatggg cgaatacgcg atgacccgcc tgcgcgagat cggcaaggat tgcccgttcg
     3901 tcagcgccat ccgcggcaaa ggcctgatgt tcggcatcga cttcgatcgc ccgaaggaat
     3961 cgttcaagct gaagatggcg tgggacatgc tgcacaagat gaattttggc gtgttcggcc
     4021 agatggtcat catcccgctg ctgcagaagc accacatcct gacgcaggtg gccgggtatc
     4081 acacggaggt gatcaagttc ctgccgccga tgacgatcac caaggcggat atggattggt
     4141 tcctcggggc gatgcaggaa acgctgatgg acacgacacg gttcccgggc gcggcgtggg
     4201 agacggtgtt cggcctggcg aagcgcgcga tgatggcgtg aacggcgaag ggtgcctctc
     4261 accaaaccca tacgggacat ccgccacgtc atcacccatc atccccacgg acatcacgaa
     4321 cctcatgctt ctcgccgccc cctccgcgac gttttaaagt gctactatgg aagcacgtcg
     4381 cagccgttgg cggacgctcg cctcggtgcg gttgatcttc attggcgtgc tcgctctggc
     4441 ggccgggatg ttctggcgct tcggtgaagc gctgctcgcc ccagccgaag ccagcgagca
     4501 gcagttggtg tacaggacca accatgcgaa gatcctcgag gcgtgccgct ggatcgaggc
     4561 aaaacgagca acgctcgggc tcaaaggcga gacgattgat aacgttggag ccgtacacga
     4621 tgcgctgccc gacgtcgtcc ggcaactgga tttggaactc attcgcgtcg aagacaccgg
     4681 cgtgatgatc tacttcggtg gcggctttgg tcattgggga ttcgaagcgc cgcacacgga
     4741 tcccacgccg caggatgatc gcgggccgtg gcaactcatc ccgggccgct ggtactggaa
     4801 cgagtggggc aggtttccgc gagatcctgc caagccggtg cgcgttcgca ccagcacgct
     4861 gatcctcgcg ggcggattaa tgctcatcgc tgccggcatc ggcggattgc gcactggcca
     4921 accacggcaa ccaggggcgt aaaaacatgg acacgacaag cctcatgctc tccgtcctct
     4981 tcggcgccgt cggcatgggc ttcctcgtct atggcaagaa ggccggccga ttgattccca
     5041 tcgtcgcggg gctggcgctg atgatcatcc cgtacatgat ctcgaacaac tacgtcctca
     5101 cgggtgtgtg tgcgggcttg acggcgtggc cgttcgtgat gcgcgatctg tgattaccac
     5161 cgcacgcaca ccgcagtggc gtcatccgcg agttggttcg acccggcgtg cgcgacgatt
     5221 gcgctaaaca gggcggctac gtcgtcttcg ttcgtcgccg ataaacaccg gcagacgccg
     5281 gccatttcga attggatgcg ctggccgtcg tcgccggcag ccggggcggg ttgttcgatg
     5341 aaaccgtcgc tgacgatcgc cagccggccg tgcggcggca gcgggatcgt ctcgatcttg
     5401 tacgtgtaat catccgcgac gccgagcggc ggaccgtcgc cgaggttcag ctccgtcatc
     5461 gcgccggcgt cgtcgaagag ggtcgcgtaa ctgtggccgg catcgacgta cgtcagcgtg
     5521 cgcgacggca gatccagcag cgcgacccag agcgtgacga accggcagtc cggccggcgc
     5581 ggggcgacga acgcgttggc cgccgtcacc gcccgggcag gctccccgtg cagttgcagc
     5641 gccgcgtgca ggaagccttg cgtggcggtc atcagcaccg acgcttccac gcctttgccc
     5701 gagacgtcgc ccagcgcgac cgcgatgcga tcgttgccga gatcgatcac gtcgaaaaaa
     5761 tctccgccga cgaatcggcc gggccggctt tcaccggtga tcgtgaacgg gccaatgctc
     5821 gtgtgccgcg gcggcaatac ccagcgctgt gcggcggcgg cggcattcag atcgtgctcg
     5881 atcatcgcct gccgccgctc gatttccacg cgcttcaggt tcgccagcgc cagccccgcc
     5941 aagcgtccga gcgcaccaca gaatgctgcc gcgtgcgggc gcatcggcgc caccatgtgc
     6001 gcatgcccgg ggcgattcgc ccgcgcatcg agatacaaaa acgccgccgg cgacgggccg
     6061 agcatcagcg gcac
//
LOCUS       DATIMH010001194         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_5771472, whole genome shotgun sequence.
ACCESSION   DATIMH010001194 DATIMH010000000
VERSION     DATIMH010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5771472"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..>3351
                     /locus_tag="VLI90_20600"
     CDS             27..>3351
                     /locus_tag="VLI90_20600"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HSV16677.1"
                     /translation="MKIRGFRIELGEIEAKLCAIAGVREAVVLARDEGGDKRLVAYVV
                     SDAPLEVAALRSRLAAELPEHMLPSAFVALAAFPLTPNGKLDRAALPAPDATAVLTRS
                     FAPPQGPIETAIATLWCELLGLDRVGRHDHFFELGGHSLLVVTLIERLRQQGLVADVR
                     AVFAAPVLADLAAAIGSAAPTQRMDAPPNRITPECAAITPDLLPLVSLTQAEIDGLIA
                     ATPGGVTNVQDIYPLAPLQEGILFHHLLQHEGDAYLLRSLIAFDTRDRLDTFLAALQA
                     VIDRHDILRTAVHWQGVSTPVQAVQRRAPLPVVELAVDGDARTALLARTDPRHVRMDL
                     TRAPLLAATIAFDAASGEWLMALLSHHLIDDNYTLQLILAEVQAVLEGRGAMLPAPLP
                     YRAFIAQVRHVPAAAHEAYFRSQLADVDAPTAPFGLLDADAHIQQAIVPLDEAFAAQI
                     RQTARQRGVTSAVLFHAAFAVMLARLGERDTVVFGTTVSGRLQGSAGADRVLGMFINT
                     LPIRVDVDGPVHALIEQTYARLSGLLAHEQAPLALAQRCSAVAAPLPLFTSLLNYRHT
                     QVVAGAQMDWPGMRVLQGEERTTYPLTVSIDDRGEGFTVVAQGVDGIDPERVAAYLLT
                     ALRGLLGAGDTAVNTLSILPASECQQVLEEFNATAVVWPEAGLVHTPIERQAAATPDA
                     VAVICEGQSLTYAELNRRANRLAHRLIALGVRPDTRVAICLERRFELVVGLLAILKAG
                     GAYVPLDPSYPSERLAYMLEDSAPMALLTQAALCDRLRAATLPTLVLDNEATGAALAQ
                     YPDIDPRTIGLTPRHLAYVIYTSGSTGKPKGVAMPQQELDNLLQWQRAEGLDTPARTL
                     QFAALGFDVAFQEIFSTLRCGGTLVLISENLRQDPAALVAFIAAQRVERLFLPFIALQ
                     NVAEAGQRTHATLPALRTVITAGEQLRLNPAIRWLFASPQRRLHNHYGPTETHVVSTH
                     TLVGDPATWPALPPIGRPIANTQIYILDRRGEPTPIGVTGEIYIGGAGVARGYLNRPE
                     LTAERFLADPFHGGRMYKTGDLGRWLADGSVEYLGRNDFQVKIRGFRIELGEIEAKLC
                     AIAGVREAVVLARDEGGDKR"
BASE COUNT          510 a         1132 c         1127 g          582 t
ORIGIN      
        1 aatacctggg tcgcaacgac ttccaagtga agatccgcgg cttccgcatc gagctcggcg
       61 agattgaagc gaagctgtgc gccatcgccg gcgtgcgcga agcggtcgtc ctggcccgcg
      121 acgagggcgg cgacaagcgc ctggtcgcgt atgtggtgag cgatgcgccg ctcgaggtcg
      181 ccgcgctgcg cagccgcctg gcggcggaac tgccggagca catgttaccc agcgcattcg
      241 tcgcgctggc tgcattcccg ttgaccccga acggcaagct cgatcgggcg gccttgccgg
      301 ccccggacgc cacggccgtg ttgacgcgga gctttgcccc tccgcaaggc ccgatcgaga
      361 ccgcgatcgc cacgctctgg tgcgagctgc tcggcctcga ccgcgtcggc cgtcacgacc
      421 acttcttcga actcggcggg cactcgctgt tggtcgtcac cctgatcgag cgtttgcgcc
      481 aacaaggcct cgtagccgac gtgcgcgcgg tcttcgccgc gccggtgttg gccgatctgg
      541 ccgccgcgat cggcagcgcc gcgccgacgc agcggatgga cgcgccgccg aaccggatca
      601 cgcccgagtg cgccgcgatc accccggacc tgttgccgct ggtgagcctc acccaagccg
      661 agatcgacgg gctcatcgcc gcaacgcctg gcggcgtgac caacgtccag gacatctatc
      721 cgctggctcc gctgcaggaa ggtattttgt ttcatcacct gttgcagcat gagggcgatg
      781 cctatttgtt gcgctctctg atcgcgttcg atacgcgcga tcggctcgat acgtttcttg
      841 ccgccctgca agccgtcatc gaccgccacg acatcctgcg cactgcggtg cactggcagg
      901 gggtgtcgac cccggtgcag gccgtgcagc ggcgcgcccc gttgccggtg gtggaactgg
      961 ccgtcgacgg cgacgcgcgc acggcgctgt tggcgcgcac cgatccgcgg catgtccgca
     1021 tggacctgac acgcgcgccg ctgctcgccg cgaccatcgc gttcgatgcg gcctccggcg
     1081 agtggctgat ggcgttgttg agccatcacc tgatcgacga caactacacc ttgcagctga
     1141 tcctggccga agtgcaggca gtgctcgaag gccgcggggc gatgctgccc gcgccgttgc
     1201 cataccgggc gttcattgcc caggtccgcc acgtgccggc cgcggcgcac gaagcgtatt
     1261 tccgttcgca gctcgccgat gtcgacgcgc cgaccgcgcc gttcgggctg ctggatgcgg
     1321 acgcgcacat ccagcaagcc atcgtgccgc tcgatgaggc gttcgctgca cagatccgtc
     1381 agactgcgcg ccagcggggg gtgacgtcgg cggtgttgtt ccatgccgcg tttgccgtga
     1441 tgctcgcccg tctcggcgaa cgcgatacgg tggtgttcgg caccaccgtg tctggtcgct
     1501 tgcagggatc ggccggcgcc gaccgcgtgc tgggcatgtt catcaacacc ttgcccatcc
     1561 gcgtcgatgt cgacggtccc gtgcacgcgc tgatcgagca aacctatgcg cggttgagcg
     1621 gtttgctcgc gcacgagcaa gcgccgctgg ctttggccca gcggtgcagc gcggtggccg
     1681 cgccgctgcc gttgttcacc agcttgttga actatcgcca cacccaggtg gtggccggcg
     1741 cccaaatgga ctggccgggc atgcgcgtgt tgcagggtga ggagcgcacc acctacccgc
     1801 tgaccgtgtc gatcgacgat cgcggcgagg ggtttacggt ggtggcgcag ggtgtggacg
     1861 ggatcgatcc cgaacgcgtg gccgcgtacc tgttgacggc gctgcgcggt ctgctcggcg
     1921 cgggcgacac ggcggtcaac accctctcca tcctgcccgc atccgaatgt cagcaagtgc
     1981 tcgaggagtt caatgccacc gcggtggtat ggcccgaagc cgggcttgtg cacacgccga
     2041 tcgagcggca agcggccgcg acgccggacg cggtggcggt catctgcgaa ggccaatcgc
     2101 tgacgtatgc cgaactcaac cgacgcgcga atcgcctggc gcaccgcttg atcgcactcg
     2161 gcgtcagacc ggacacgcgc gtggcgattt gtctggaacg ccggtttgag ttggttgtcg
     2221 gcttgctggc gatcctcaag gccggcgggg cgtacgtgcc gctggatccg agttatccgt
     2281 ccgaacggct cgcttacatg ctcgaggaca gtgcgccgat ggcgttgctg acccaagcgg
     2341 cgctctgcga ccgcttgcgg gcggccaccc tgccgacgct cgtgctcgat aacgaggcga
     2401 ccggagccgc gctggcgcaa tatcccgaca tcgatccccg cacgatcggt ctgacgccgc
     2461 gccacctcgc ctacgtcatc tacacctccg ggtccaccgg caaacccaag ggcgtggcga
     2521 tgccgcagca ggagctggac aatctgctgc aatggcaacg cgccgaagga ctggatacgc
     2581 cggcccgcac gctgcagttt gccgcgctcg gtttcgacgt cgcttttcaa gagatcttct
     2641 cgacgctgcg ttgcggcggc acgctggtgc tgatcagcga aaacctgcgc caggatccgg
     2701 cggcactcgt ggcgttcatc gcggcgcaac gcgtcgagcg gctgttcctt ccgttcatcg
     2761 cgctgcagaa cgttgccgaa gccgggcagc gcacacacgc cacgctgccg gcgttgcgta
     2821 cggtcatcac tgcgggtgag caattgcgcc tgaatccggc gatccgctgg ttgttcgcat
     2881 cgccgcagcg ccggctgcac aaccactatg ggccgaccga aacgcatgtg gtgtcgacgc
     2941 atacgttggt cggtgacccg gcgacgtggc cagcgctacc gccgattggc cgcccgattg
     3001 ccaatacaca aatctacatt ctggatcgtc gcggggagcc gacgccgatc ggggtgactg
     3061 gggagatcta catcggtggg gcgggtgtag cgcgcggcta cctcaaccgc ccggaactga
     3121 cggccgagcg cttcctcgcc gatccgttcc acggcggccg gatgtacaag accggcgacc
     3181 tgggtcgctg gctggctgac ggttcggtcg aatacctggg tcgcaacgac ttccaagtga
     3241 agatccgcgg cttccgcatc gagctcggcg agattgaagc gaagctgtgc gccatcgccg
     3301 gcgtgcgcga agcggtcgtc ctggcccgcg acgagggcgg cgacaagcgc c
//
LOCUS       DATIMH010001195         4418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2595029, whole genome shotgun sequence.
ACCESSION   DATIMH010001195 DATIMH010000000
VERSION     DATIMH010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2595029"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VLI90_20605"
     CDS             <1..674
                     /locus_tag="VLI90_20605"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HSV16678.1"
                     /translation="VVALFVPLIISSGGNSGSQGTSLIIRSLALKELRLKDWWRVFGR
                     ELRTGLALGLFLGIIGFCRIGAWQGLSHVPHVGSFFQTHGSQDELNIPEGMKQISGDA
                     ELAQPLAVPAFSLPVGAHVSKGMLIPEATAMPTDVKTITRTSRTATAYGEHWLLVGLT
                     VFGALIGVVAWGSLAGSMLPFILRRVGFDPATSSAPFVATLVDVTGLIIYFMVAKIIL
                     TGTLL"
     gene            671..1591
                     /locus_tag="VLI90_20610"
     CDS             671..1591
                     /locus_tag="VLI90_20610"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HSV16679.1"
                     /translation="MTDAPLPRPTSVRDIRRREDRGIYALSFFLPPVKREAAYAVLAF
                     RRLIHSAIDNSDQPGDSVDLRLDLFRDRLEEIYAGRLELPERPFRDEAQHTLAAIAAV
                     VRQFEIPKQHFLDLAEARRMELTVSRYATWNALQQYLDRAAGSIARVMAGVLGVRHSE
                     AAERLATLSQAIQLTAILCCLGDVGGNGTIFLPLEDLARLKYSERQLQSRVANEALAP
                     LIKFEIDRARDLYRRGAEGICWLADDGSRFAVAAFAVLNVRRLKVIEAQPYNVFARRP
                     ALRPHQQLACVAAAWRLARRTEDHPLPDVF"
     gene            1603..2601
                     /locus_tag="VLI90_20615"
     CDS             1603..2601
                     /locus_tag="VLI90_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16680.1"
                     /translation="MPTIPLYTPAVTPLGSHGVTAPGGYEWWRFDAEDAASDLRLLAD
                     FFDGHPFHPGYLRAYAHYRRWPTRIAPPLPRDYRCVRFALYRAGKIVARFQSRHDPAE
                     FSASPAATEISIGSNHILAQPDGSLHVSLRGVTRAMQQVSAQLQFRPRPNIPVSAEQR
                     IGHQHWVVAQPLCDVEGTVNVFSPSAPATPEVTMFQGVGGHDHSFGTRPFGDDAARWM
                     FGRVLMADRAVPFRIAWPSDEAAQPEVRLIDTTSAVETPHIECIGWRSTPRLLRYPLH
                     VKFGDRIQLRNPQLIESATVSASVVYDATVDTSTGPARCELVSPQRLRWKWRSTVS"
     gene            complement(2700..>4418)
                     /locus_tag="VLI90_20620"
     CDS             complement(2700..>4418)
                     /locus_tag="VLI90_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16681.1"
                     /translation="ETPVLRVRQFAFPRNRADDDWTMILGLMGRSMTAAAAAPIWRLR
                     RSLILLVALVAAVATLPGCINMPTLLPASKQRPIDRKVVEYPSGFVLLPLVNDLNSPT
                     AMAFDQDQNLLVAESGGGEPHIFGWHRDGTYFNIYPWKRNVSFYPAANTVLYGPIGGM
                     VAHGGRIYVSHHDRNGLGVITALGYDGTQTTIVAGLPAQGDNGVTDLVFSPLDGRLWF
                     GVGTATNSGVVGNDNWNEGWLKRYPNVHDRVYSPNTPPTPYKLRGYRFDSKNPWAGLF
                     GGADIARTGPFQPFGVSDQARIEPTDKPNGAIYSVAADGGDMRVEAFGIHNPRGLAFS
                     GAQVLYFTNDGMQLRGTRPVKYDPDSLLKCGRGLWYGWPDYTTNGNPVSAPEYQPPIE
                     LLARSGYTENSALIDQESSGLPLNVAATVQGQVAGVFPTMSGAAKLDFAPENGPFREF
                     FHSAIVALDGDQAPFATSGVKDFRGPIGHKVVIVDLSTKTVKDFIRNTAELPASMQAF
                     GTVALERPIDVKFGPDGSLYILDFGRMENKNGIPRLFPNTGRIFKLVPVATPATSTTV
                     APAESH"
BASE COUNT          735 a         1408 c         1457 g          818 t
ORIGIN      
        1 ctgttgtggc gctctttgtg ccgctgatca tctcctccgg cggaaactcc ggctcgcagg
       61 gaacatcgct catcatccgc tcgctggcgc tgaaagaact tcgcctgaaa gactggtggc
      121 gcgtcttcgg ccgtgagctg cgcacgggtc tggcgctggg cctgttcctc ggcatcatcg
      181 gcttctgccg catcggcgcc tggcagggcc tctcgcacgt gccgcacgtc ggctcattct
      241 tccaaacgca cggctcgcag gatgagctga acattcccga agggatgaag caaatttccg
      301 gcgacgcgga actggcccaa ccgctcgcgg tgcccgcgtt ctcgctgccg gtcggcgcgc
      361 acgtcagcaa gggcatgctg atccccgagg cgacggcgat gccgacggac gtgaaaacga
      421 tcacccgcac ctccaggacg gcaaccgcgt atggcgaaca ttggctgctc gtcggcctca
      481 ccgtcttcgg cgcgctgatc ggcgtcgtcg cgtggggctc gctagccgga tcgatgctgc
      541 cgttcatcct tcgccgcgtc ggcttcgacc ccgcgaccag ctccgcgccc ttcgtcgcga
      601 cactggtcga tgtgacgggc ctgatcatct acttcatggt cgcgaagata atcctcaccg
      661 ggacgctgct gtgaccgacg cgccgctgcc gcggccgaca agcgtgcgcg acatccgccg
      721 ccgcgaagac cgcggcatct atgcgctcag ctttttcctg ccgccggtca agcgcgaggc
      781 cgcatacgcc gtcctcgcgt tccgaagatt gatccacagc gccatcgaca attccgatca
      841 acccggcgac agcgttgatc ttcgtctcga tctgtttcgt gatcgactag aagagattta
      901 cgcgggccgg ctggaactgc cggagcggcc gtttcgggat gaggcgcagc acacgctcgc
      961 ggcaatcgcc gctgtcgtgc gacagttcga gattccgaag cagcatttcc tggacctggc
     1021 cgaggcgcgg cggatggagc tgacggtgtc gcgctacgcg acctggaacg cgctgcaaca
     1081 atatctcgat cgggccgccg gttcgattgc ccgcgtgatg gctggcgtgc tgggcgttcg
     1141 ccatagcgaa gccgccgagc gattggcaac gctgagccaa gccattcaac tgactgcgat
     1201 cttgtgttgt ctcggcgatg tgggcggcaa tgggacgatt tttctgccgc tggaagactt
     1261 ggcgcgattg aagtacagcg agcggcagct tcaatcgcga gtggcgaacg aagcgcttgc
     1321 cccgctgata aaattcgaga tcgaccgggc gcgcgacttg taccgccgtg gcgccgaagg
     1381 catctgctgg ctggcggatg acggctcgcg cttcgcagtg gctgcattcg ccgtgctcaa
     1441 cgtgcggcgt ctcaaggtta tcgaagcgca gccgtataac gtcttcgcgc gccgaccggc
     1501 gctgcggccg catcagcagt tggcttgcgt cgccgccgcg tggcggctgg cgcggcgaac
     1561 cgaagaccat ccgctgccgg atgtgttcta atccacctgt ccatgccgac gatcccgctc
     1621 tacacccccg ccgtcacacc cctcggttcg cacggcgtca ccgcgcccgg cggatatgag
     1681 tggtggcgct tcgacgccga agatgcagcg agcgacctgc gactgctcgc agattttttc
     1741 gacggccacc cattccaccc gggctacctg cgcgcttacg cacactatcg ccgctggcca
     1801 acgcgcatcg ccccgccgct gccgcgggat taccggtgcg tgcggttcgc gctctatcgc
     1861 gccggcaaga tcgtcgcgcg gtttcaatca cggcacgacc cggcggagtt cagtgcatcg
     1921 cccgccgcga cggagatatc gatcggctcc aatcatattc tggcccaacc ggacggttca
     1981 ctccacgtct ccctccgcgg cgtgacgcgg gcgatgcagc aggtgtcggc gcaattgcaa
     2041 ttccgcccgc ggccgaacat tccagtatcc gcagaacaac gcattggaca tcagcattgg
     2101 gtcgtcgcgc agccgctgtg tgatgtggag gggaccgtca acgtcttcag cccgagcgcg
     2161 ccggcgacgc cggaggtgac tatgttccag ggcgttggcg gccacgacca cagcttcggc
     2221 acgcggccgt ttggcgacga tgcggcgaga tggatgttcg gacgagtgct aatggccgat
     2281 cgcgcagttc cgtttcgcat cgcctggccg agcgatgaag ccgcacaacc cgaggtgcgt
     2341 ttgatcgaca cgacaagtgc agttgagacg ccgcacatcg aatgcatcgg atggcggtcc
     2401 acgccgcggc tgctgcggta tccactgcac gtgaagttcg gtgacaggat tcaactgcgg
     2461 aatccgcagt tgatcgagtc agcaacggtc agtgccagcg tcgtatatga cgcgacggtc
     2521 gatacatcga ctgggccggc gcgatgtgaa ctggtgagtc cgcagcgcct gcgctggaag
     2581 tggcgttcta cggtttcatg atccacgccg ggtccggatt ctgcggcgcg tcgagtaaga
     2641 agcatccggg tcgtcggagt acctctgaac ccggcgtgtg gggggggact gcaatgtgtt
     2701 cagtgcgatt cagctggggc gaccgtcgta ctcgtggcgg gcgtggcgac ggggacgagc
     2761 ttgaagatcc ggccggtgtt ggggaacagc cggggaatgc cgttcttgtt ttccatgcgg
     2821 ccgaaatcca ggatgtacag cgatccatcg ggcccgaact tcacgtcgat cgggcgttcc
     2881 agcgcgaccg tgccgaaggc ttgcatgctc gcgggcagct cggcggtgtt gcggatgaag
     2941 tccttcacgg ttttcgtgct caggtcaacg atgaccacct tgtggccgat cgggccgcgg
     3001 aagtccttga cgccgctcgt ggcgaatggg gcttggtcgc catccagcgc gacgatcgcg
     3061 ctgtggaaga actcgcggaa cgggccgttc tcaggcgcga agtccagctt ggcggcgccg
     3121 gacatcgtcg gaaagacgcc ggcgacctgc ccctgcacgg tcgcggcgac gttcagcggc
     3181 aagccgctgg attcctggtc gatcagcgcg gagttctcgg tgtatccgct gcgcgccagc
     3241 agttcgatcg gcggttgata ttcgggggcg ctgacggggt tgccgttggt cgtgtaatcg
     3301 ggccagccat accacagccc gcggccgcac ttcagcaggc tgtcggggtc gtacttcacc
     3361 gggcgcgtgc cgcgcagctg cattccgtcg ttggtgaagt acagcacctg agcgccgctg
     3421 aacgccagcc cgcgcgggtt gtgaatgccg aacgcctcga cgcgcatgtc gccaccgtcg
     3481 gcggcgacgg aataaatcgc gccgttgggc ttgtccgtgg gctcgatgcg cgcctggtcg
     3541 ctcacgccaa atggttggaa cggcccggtg cgcgcgatgt ccgccccgcc aaataaaccg
     3601 gcccaggggt tcttcgagtc gaagcgatag ccgcgcagct tgtacggcgt gggcggcgtg
     3661 ttgggcgaat agacgcggtc gtgaacattc gggtatcgct tcagccagcc ttcgttccag
     3721 ttgtcgttgc cgacgacacc gctgttggtc gcggtgccga cgccgaacca cagccggcca
     3781 tcgagcgggc tgaagacgag gtcggtcacg ccattgtcgc cctgcgccgg caagccggcg
     3841 acgatcgtcg tctgcgtgcc gtcgtaaccg agcgcggtga tgacgccgag cccgttgcgg
     3901 tcgtgatgcg aaacgtaaat ccgcccgccg tgcgcgacca tcccgccgat cggtccatac
     3961 agcaccgtgt tggccgcggg atagaagctg acgttgcgct tccaagggta aatattgaaa
     4021 taggtgccgt cgcggtgcca gccaaagatg tgcggctcgc ccccgccgct ctcggcgacg
     4081 agcaggttct ggtcctgatc gaatgccatg gccgtcgggc tattcaggtc gttgaccagc
     4141 gggaggagga cgaagcccga gggatactcg accaccttgc ggtcgatcgg ccgctgctta
     4201 ctggcgggca gcaaggtcgg catattgatg cagccgggca gcgtcgccac cgccgccacc
     4261 agcgcgacga gcaggatcag cgatcggcgc aatcgccaga tcggagcggc ggcggcggcg
     4321 gtcatagatc gtcccatcag ccccaggatc atcgtccaat catcatccgc gcggttgcgg
     4381 gggaatgcaa attgccgcac tcgtagcacg ggcgtctc
//
LOCUS       DATIMH010001196         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2595482, whole genome shotgun sequence.
ACCESSION   DATIMH010001196 DATIMH010000000
VERSION     DATIMH010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2595482"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VLI90_20625"
     CDS             complement(<1..229)
                     /locus_tag="VLI90_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16682.1"
                     /translation="MTGVFRMAAGLALLAGMLCTAPRAEAAALTGDTITGTLTFCKLG
                     ASGNNFTPVSIVAPDTFSWTDGANVDSAVFSD"
     gene            442..>1554
                     /locus_tag="VLI90_20630"
     CDS             442..>1554
                     /locus_tag="VLI90_20630"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSV16683.1"
                     /translation="MRRRTFLGGAAASVPLSRAIAQTGKASVLRYVPQADLTVIDPVM
                     TTAYITRYHATAVWDQLYGVDSDLKPQPQMVDGHTVEDDGKLWTFTLRDGLKFHDGEP
                     VRGRDCVASIKRWAARDPLGQILLTRVAEMAAPDDKRFTIRLNKPFSLLLNCLSKIGP
                     PTLVVMPERFAQTDPFQAIKEIVGSGPYRFVANERVVGSRVVYTRNADYVPRPSGTPN
                     WLSGPKITHFDRVEWVIQPDTGTAAAALQNGEVDWWENPPVDLLPVLDKSAGVRTEDS
                     GPLGILGSGIFNHLYPPFDKPAVRRVILEAASQADFMTAVAGTNTAMWRDGVGFFAPN
                     TPMANTAGTEIFKTRRDLPTLKKSLRDAGYNGEKIVM"
BASE COUNT          290 a          510 c          492 g          262 t
ORIGIN      
        1 tgtccgagaa cacggctgaa tcgacattgg cgccatcggt ccaggagaac gtatccggcg
       61 ccacgatcga caccggggtg aagttgttgc cgctggcgcc gagtttacaa aacgtgagcg
      121 taccggtgat cgtgtccccc gtcagtgcgg ccgcctcggc tcgcggtgcc gtgcacagca
      181 tacccgccag aagcgccaga ccagccgcca tccggaacac gcccgtcatg atcgtcgtcc
      241 ccaacattcg ccgcttcggt atccccggaa gccggtttcc accggctttc atcttcgcgc
      301 ccgcgaagcc cagggacgga gcgtcggaaa ggctgactct ggcacgtgat ttgcttgcac
      361 tgtcctcggg gttgatcgcg ggatgttctc gcgagagccc gttcgggcca tccggcccgc
      421 gatagccagg ggagaccgat catgcggcga cgtacgttcc tcggcggggc ggcggcatcc
      481 gtgccgctct cgcgtgccat cgcccaaacc ggcaaagcga gcgttttgcg ctacgtgccg
      541 caggctgacc tgaccgtgat cgatccggtg atgaccaccg cttacatcac gcgctaccac
      601 gccaccgcgg tgtgggatca attatacggg gtcgacagcg acctgaagcc gcaaccgcag
      661 atggtcgacg gccacacggt cgaggacgac ggcaagctgt ggaccttcac cctgcgcgac
      721 ggcctgaaat tccatgatgg agagccggtg cgtgggcgag attgcgtcgc ctccatcaaa
      781 cgatgggcgg cgcgcgatcc gctggggcag attctgctca cgcgcgtggc ggaaatggcc
      841 gcgccggatg ataagcgctt caccatccgg ctgaacaagc cgttcagcct gctgttgaac
      901 tgcctctcga agatcggccc gccgactctg gtggtgatgc cggaacgctt cgcccagacc
      961 gatccgttcc aggcgatcaa ggagatcgtc ggcagcggcc cgtaccgttt cgtcgccaac
     1021 gaaagggtcg tcggcagcag ggtcgtctac acgcgcaacg ccgattacgt gccgcggccg
     1081 agtggcacgc cgaactggtt gtccggccca aaaatcacgc atttcgaccg ggtggagtgg
     1141 gtgatccagc cggacaccgg caccgccgcc gccgccttgc aaaacggcga ggtcgactgg
     1201 tgggaaaacc cgcccgtcga tctgctgccc gtgctggaca aaagcgcggg cgtgcgcacc
     1261 gaggacagcg gccctctggg cattctgggc tcggggatct tcaatcatct gtatccaccg
     1321 ttcgataaac cggcggtgcg gcgcgtcatc ctggaggcgg cttcgcaggc cgatttcatg
     1381 accgccgtcg ccggaacgaa cacggcgatg tggcgcgacg gcgtcggctt tttcgcaccc
     1441 aacacgccga tggccaacac cgccggcacc gaaatcttca agaccagacg cgacctgccg
     1501 accctgaaga aatccctgcg cgacgcgggc tataatggcg agaaaatcgt tatg
//
LOCUS       DATIMH010001197         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2883391, whole genome shotgun sequence.
ACCESSION   DATIMH010001197 DATIMH010000000
VERSION     DATIMH010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2883391"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VLI90_20635"
     CDS             complement(<1..1024)
                     /locus_tag="VLI90_20635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16684.1"
                     /translation="MQTATVAQGRSISDQIGSNHTRRARSARRALLLAAAAVTVGSVA
                     QSSHATSYNWDPGHVAATTGGGTGTWDTASSNWFNSTDQVWPNTNPNTDNAIFGNAAT
                     AATVTIQGASAAALNVFANQVTFSSANYTLTGSAGSTLTLSGTAPAISTSGGTAAISV
                     PIVLGANANFTTTNTLTLNSGAVISGNFGITHVGPGFLTLVSNSSFSGGINNISGSIG
                     LQIAPTTSTASAIGSGTFTSAGGVSMNNNLTSTAIQLINNLQSYSAATNVLFSAGSTP
                     YLNMGTGAATLTGNTTFSVRFGSVGSGQTPGGTFVIGGNVGETGGSFGITMGSPATVN
                     SVANVIA"
     gene            complement(1490..>4351)
                     /locus_tag="VLI90_20640"
     CDS             complement(1490..>4351)
                     /locus_tag="VLI90_20640"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HSV16685.1"
                     /translation="NTYAGNTNVNAGMLILSGGGSIASGNFNVAGGAVNVNGGGSLGS
                     ANINVNSGSLSIAPGGALTNLSPIVTVAGGASMSVAAGATIPTSLSVVDNGTVTLNGA
                     TTLGTLSGSGVVTLNGANLTIASGGTVGNVLQDGGSAGSLTVNAGSPLILTGANTYSG
                     ATAINSGATLQIGNGGATGGLAATSAITNAGTLVYNLAAPSAVTIANAIANTGTLNFN
                     SAGDLNVTGAITGSGTLRQTGAGTTSLSGDISGFTGGITLGGGTLVFNYAAPTTVSNA
                     IGVVAGATSAVRNVGKLTMSGVESVGSTAGAITYNVTPIAGNMPSGVPADVEITGSIN
                     RTSGSDGVGIIKTGTGTLAISGATDNASLGATVNGGTLLLNKTSATTIHAIGGGGLVI
                     NNGGTVKITGTGGEQIYNAAGTTTIADGGTFDLNGQSQGLNNMTLGNATGAGLLTTSV
                     PGLAVVAKIGTAVGTANDAGASAAATIGRLSLGGDSNISAASGASIELQNNGLSATGG
                     NHTLSDIGPGTVLLTGLADNSAVNVAVTSGTLVLAKPSVAGTATGSVHSASSVTGVSA
                     GATLQLAGSGGDQLFDGFAVASGTQPDYGVLNMNGTFDMNGQSEGFDKLTGTGNVINS
                     QSGTPVTLTLGANNGLGAATPEFDGVISGNIALIKADATTPPATEVPLILGGANTYTG
                     NTNVSAGVLTIAAGGSIASANVVVAASANPGTLPPAVLNVNGSLASNANVTANGPVNF
                     GGTTGATANTQALSSLAIGSGVTITITPSVGPLHPTILAPAATTYTDASSLIDITNNA
                     FISNGSAASALVQLQTGQIFSSVAPDLIHAIGYINLSGGRFEVRYTLKGDSNLDGVVN
                     VGDLGALATSYGITGGMSWGNGDFNRDGNVDVGDLGALATNYGSQLASGPSAESAALV
                     ASPLMLTAAPSAIAAVPEPASLGVLAISAIGILANRRRR"
BASE COUNT          730 a         1440 c         1366 g          815 t
ORIGIN      
        1 cggcgatgac gttggcaacg ctgttgacgg tggcggggct tcccatcgtg atcccgaagg
       61 acccgccggt ctcaccgacg ttgccgccga tgacgaacgt gccgcccggc gtttgcccgc
      121 tgcccacaga tccgaaacgg acgctgaacg tggtattgcc cgtgagcgtc gcggcgccgg
      181 tgcccatgtt caggtacggc gtactgccgg cggagaacag gacgttggtg gccgccgagt
      241 agctctggag gttgttgatg agttgaatcg cggtgctggt gaggttgttg ttcatcgaca
      301 caccgccagc ggacgtgaag gtcccgctgc cgatcgccga ggcggtgctg gtggtcggcg
      361 cgatctgcaa cccgatcgaa ccgctgatgt tgttgatgcc gccactgaag ctgctgttgg
      421 agacaagcgt caagaatccg ggcccgacgt gcgtgatgcc gaagttcccg ctgatgaccg
      481 cgccgctgtt gagcgtgagc gtgttggtcg tagtgaagtt cgcgttggcg ccgagaacga
      541 tcgggacgct gatggcggcc gtcccaccgc tggtgctgat ggctggcgcg gtgccgctca
      601 acgtcagcgt gctgcccgcg ctgccggtca gcgtgtaatt agcactgctg aaggtcacct
      661 gattggcaaa gacattgagc gcagcggcgc tggccccctg gatggtgacc gttgccgccg
      721 tcgcggcgtt gccgaagatc gcgttgtcgg tattgggatt ggtgttcggc caaacctggt
      781 cagtcgagtt gaaccagtta gagctggccg tgtcccacgt gcccgtcccg ccgcccgttg
      841 tagcagcgac gtgaccggga tcccagttgt acgacgtggc gtgactgctc tgcgccaccg
      901 acccaaccgt gaccgccgcc gccgcgagta gcaacgcccg ccgggccgaa cgcgcgcgac
      961 gcgtgtggtt cgagccgatt tgatcagaga tagaccgacc ctgcgctacg gtagccgtct
     1021 gcatattttc tctccgccgt ctgacaaaag ggaagtgggt gtgaaagccc gatacgacca
     1081 atatataggc agtcggggca aaaacaatga ttaacttcca tgaccgatga aatatttcac
     1141 tcgggggcaa ggaagtggga cgggctgctt gagaattgga cgccaagaca ttagtgcaac
     1201 atttgcaaca tcttaagata gacaccgcca cccatcgctc aaatgtggaa taccagcagg
     1261 ctgatgcaga gatttgcggt gttattccgg atggactatt ccggctatcg cgaacgtaag
     1321 caagaacata aacagcaaga ctatttcccc gaacggtgag cgatttcaca agggagcaac
     1381 ttggatctca tgccgctctc gctcctcttt cgcgaaaaaa ggcggcggct cgccggtcag
     1441 gagcaagccg ccgcagcgga gggggtgagt atcacggtca catccggcgt catcgccggc
     1501 ggcgattggc gagaattccg atcgccgata tcgctaggac gccaagcgac gccggctcgg
     1561 gcaccgcggc gatcgccgag ggtgccgccg tcaacatcaa aggactggcg accaaggccg
     1621 ccgactcagc ggatggaccg ctggccagct gcgagccgta gttggtcgcg agcgcgccca
     1681 gatcgccgac atcgacattc ccgtcgcgat tgaaatcacc attgccccag ctcatcccgc
     1741 cggtgatgcc gtacgacgtc gccagcgcgc ccaggtcgcc aacgttgact acgccatcga
     1801 ggttcgagtc gcccttcagc gtgtagcgga cttcgaatcg cccgccactc aggttgatat
     1861 aaccaatcgc gtgaatcagg tccggcgcga ccgagctgaa gatctgccca gtctgcagct
     1921 gcaccagtgc gctcgccgcc gagccgttgg agatgaacgc gttgttggtg atgtcgatca
     1981 gcgaagacgc gtcggtgtac gtcgtcgcag ccggcgccaa aatcgtcgga tgcaacggcc
     2041 cgaccgacgg ggtgatcgtg atggtgaccc cgctgccgat cgcgagcgac gacaacgcct
     2101 gcgtgttagc cgtcgcgccg gtcgtcccac caaaattcac cgggccgttc gcggtgacgt
     2161 tcgcgttcga cgcgagcgac ccgttgacgt tcaacaccgc cgggggcaac gtgccggggt
     2221 tggcgcttgc cgcgacgaca acgttcgccg acgcgatgct gccaccggcg gcgatcgtca
     2281 gaacgccggc gctgacgttc gtattcccgg tgtaggtgtt cgcgccgccg agaatcaacg
     2341 gcacctcggt cgccggcggt gtcgtcgcgt cggctttgat cagcgcgatg ttgcccgaga
     2401 taacgccgtc aaactccggc gtcgcagcgc cgagcccgtt gttcgcgccc agcgtcagcg
     2461 tcaccggcgt gccgctctgc gaattgatta cgttcccagt gccggtcagc ttgtcgaaac
     2521 cttcgctctg gccgttcatg tcgaacgtgc cgttcatgtt caacacgcca taatccggct
     2581 gagttccaga agcgaccgcg aagccatcga agagttgatc gcctccgctg ccggccagct
     2641 gcagcgtcgc gcctgcgctc acaccggtga cgctcgatgc cgaatgcacg ctgcccgtcg
     2701 ccgtgcccgc cacgctgggc ttggccagca ccaacgtgcc gctcgtcact gcgacgttga
     2761 ccgccgaatt atccgccagc cccgtcagta acaccgtgcc gggaccgatg tcgctcagcg
     2821 tgtgattacc gcccgttgcc gacaatccgt tgttctgcag ctcaatactc gcgccgctcg
     2881 ccgcgctgat gttgctgtcg ccgcccaggc tcaatcgccc gatcgtcgcc gccgccgatg
     2941 cccccgcatc gttcgccgtg ccgaccgcgg tgccaatttt ggccaccacc gccaagccgg
     3001 gcacgctcgt cgtcagcagc cccgcgccgg tcgcattgcc cagcgtcatg ttgttcaagc
     3061 cctggctctg cccgttcagg tcgaacgtgc cgccatcggc aatcgtcgtg gtccccgcgg
     3121 cgttgtaaat ctgctcgccg cccgtgccgg tgatcttgac ggtgccaccg ttgttgatga
     3181 ccagcccccc gccgccgatc gcgtgaatgg tcgtcgccga tgtcttgttc agcagcagcg
     3241 tcccgccgtt gacggtcgcg ccaaggctgg cgttgtcggt ggcgccggaa atcgccagcg
     3301 tgccggtgcc ggtcttgatg atgccgacgc catcggaccc gctcgtgcga ttgatcgagc
     3361 cggtgatctc cacatccgcc ggcacaccgc tgggcatatt gcccgcgatc ggcgtgacgt
     3421 tgtaggtgat cgcccccgcc gtgctgccga ccgattcgac gccgctcatc gtcagcttgc
     3481 caacgttgcg aaccgcgctg gtcgcgccgg cgacaacgcc gatcgcgttc gacaccgtcg
     3541 tgggcgccgc atagttgaag accaacgtgc cgccgccaag cgtgatgccg ccggtgaagc
     3601 cgctgatgtc accgctgagc gatgtcgtgc cggcgccggt ctggcgaagc gtcccgctgc
     3661 cggtgatcgc gccggtcacg ttcagatcac ccgcgctgtt gaagttcaac gtgccggtgt
     3721 tggcgatcgc gttcgcgatc gtcaccgccg acggcgctgc caggttgtac accagcgtcc
     3781 cggcgttcgt gatcgcggat gtcgccgcca aaccgccggt ggcgccaccg ttgccgatct
     3841 gcaacgtcgc accgctgttg atcgccgtcg cgccgctgta cgtgttcgcc cccgtcagga
     3901 tcaacggcga acccgcgttc accgtcaaac tgccggcgct gccgccgtcc tgcaaaacgt
     3961 tccccaccgt cccaccgctg gcaatggtca ggttcgcgcc gttcaaggtt accacgccgc
     4021 tgccgctcag cgtgccgagc gtcgtcgcgc cgttcagcgt gaccgtgccg ttatcgacaa
     4081 cgctgagcga cgttggaatc gtcgcaccgg ctgccacgct catcgacgcc cccccggcga
     4141 ccgtgacgat cggtgacagg ttcgtcagcg cgccgccggg tgcgatcgac agtgagccgg
     4201 aattgacgtt gatgttcgcg ctgcccagcg acccgccgcc gttcacgttc accgccccac
     4261 cggcgacgtt gaagttgccg ctcgcgatcg acccgccgcc gctcaggatc agcatgccgg
     4321 cattcacgtt cgtattgccg gcgtacgtgt t
//
LOCUS       DATIMH010001198         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1151985, whole genome shotgun sequence.
ACCESSION   DATIMH010001198 DATIMH010000000
VERSION     DATIMH010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151985"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1970
                     /locus_tag="VLI90_20645"
     CDS             75..1970
                     /locus_tag="VLI90_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16686.1"
                     /translation="MKHQTQRRTNLILAAALSALPILAATAHAQYRVGDDGRANDANN
                     RLGSGGYNSGRDASQRPYSSFGNQIVTGNSTGLSYFHGNSQVTDPLEFRGSTATGASD
                     HFLAVSGPVNYAQRSTGQSQYQPYYSTARLAAPPPPGFQETPGGAGLVPAPPITNQPN
                     DTRLGIINSAEVDTTQLPPAGVGDWAGPVDPSATAAITASPLTGVRSLNADDPTGASY
                     MSRVNSVRPDTPGDRARLDNVQVARLRAELNDTMVTPDGQPLDNTAGPQQIGVPPTAA
                     QQRGQQRGGAQPGANNGAGAVDLTQAQAMSSGQNLTANATLTPQALNPSVNNSVINNG
                     AANQQQGITQRMLIAPEEQSAQLAELQRRYQQSHGQMNAQEANQLYNKEVAAKNEAEQ
                     NPPTDNPQANKTATAGGATGAGANTSVPPPPVGPDYAKANQDIMKKAQEPLNPVLKGP
                     LNQNGNQPYVITSLATGIKAKGLSDLMKQAEEQMRQGKFTNALDTYDSAQQVAPNNPL
                     IALGRGFAELGASYYGKAEGDLRRGIFAAPAVLVGQYDLKGFLGEDRLKFVVKDLKDI
                     LKTEKTERPAFLLAYIFHNVGDDESTAKYLDEADRRVAGRDGMVNLMRNTWGLKKSDT
                     PATPANP"
     gene            2017..>2655
                     /locus_tag="VLI90_20650"
     CDS             2017..>2655
                     /locus_tag="VLI90_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16687.1"
                     /translation="MNSDRTVGFGIRWRHVSLLIAIVVLLALAGRQWHFVDQYSVNMM
                     CWDQWDDYWPMFRGQGWWACFNFQPGPQRQGAGELLMRCVAAASGWNSRWDSFSISAT
                     LTAAAIAAMALVWIADVRGVALIALPLLFFNVRQYQQLVADANVAHGAMPILLLMLYG
                     LAWYARPITLRAALLVFLTFLLIFTGYGIFAGLLTPIVLIVELAQETGAGQRR"
BASE COUNT          519 a          875 c          850 g          411 t
ORIGIN      
        1 ccaaacgtcc tacaatccga tataatctac agttgccacg gcgaatccct ggacgcgatt
       61 tcaaaggtgc ggcgatgaag catcaaacac aacgtcgaac gaatctgatc ctcgcggcgg
      121 ccctgtcagc gctgccgatc ctggctgcca ccgctcacgc gcaataccgc gtcggcgatg
      181 acggtcgcgc caatgacgcc aataatcgtc tcggcagcgg ggggtataac agcggtcgcg
      241 acgccagcca gcggccgtat tcatcgttcg gcaaccagat cgtcacgggc aactcgacgg
      301 ggctgagcta cttccacggc aactcgcagg tgaccgaccc gctggagttt cgcggatcga
      361 ccgcgacggg agcctccgat cacttcctgg ccgtctcggg gccggtgaat tacgcgcagc
      421 gatcgaccgg gcaatcgcag tatcagccgt actacagcac ggcgaggctc gccgcgccac
      481 cgccgcccgg tttccaggag acgccgggtg gagcgggcct ggtccccgcg ccgccgatca
      541 ccaatcagcc gaacgacacg cgcctgggca tcattaattc cgccgaggtg gacacgacgc
      601 agctgccccc ggccggtgtg ggtgactggg cgggaccggt cgatccatcc gccaccgcgg
      661 cgatcaccgc gtcgcccctg accggcgtgc gctcgctcaa cgcggacgat cccaccggcg
      721 cttcatacat gtcacgcgtc aacagcgtgc ggcccgacac gccgggtgat cgcgcgcgtc
      781 tggataatgt ccaggtggct cggcttcgcg cggagctgaa cgacacgatg gtcacaccgg
      841 atggtcagcc gctggacaac acggctggcc cgcagcagat cggcgtgcca cccaccgccg
      901 cccagcagcg cggacagcag cgcggcggcg ctcagcccgg cgccaacaac ggcgctggcg
      961 cggtcgatct gacccaggcg caggcgatgt cgtcggggca gaacctgacg gccaatgcca
     1021 cgctcacgcc gcaggcgctg aatccgtcgg tgaacaacag cgtgatcaac aacggcgcgg
     1081 cgaatcagca gcagggcatc acgcagcgga tgttgatcgc gccggaagag cagagcgcgc
     1141 agctggccga gctgcaacgc cgctatcagc aatcgcacgg gcagatgaac gcccaggagg
     1201 cgaaccagct gtacaacaaa gaagtcgccg ccaagaacga agccgagcag aatcccccga
     1261 cggacaaccc gcaagcgaac aagaccgcga cggctggcgg cgccacgggc gccggcgcga
     1321 acaccagcgt cccaccgccg ccggtgggtc ccgattacgc caaggcgaat caggacatca
     1381 tgaagaaggc gcaggagccg ctgaaccccg tgctaaaggg cccgctgaat caaaacggca
     1441 atcagccgta cgtcatcacc agcctggcga ccggcatcaa agccaaaggc ttgtctgatc
     1501 tgatgaagca ggcggaagag cagatgcggc aaggaaaatt caccaacgcg ctggacacgt
     1561 acgacagcgc gcagcaggtc gcgccaaata acccgctgat cgcgctcggc cgcgggtttg
     1621 ccgaactcgg cgcttcgtac tacggcaaag ccgaaggcga tctccgccgc ggcatcttcg
     1681 ccgccccggc ggtgctcgtc ggtcagtacg acctcaaggg cttcctcggc gaagaccggt
     1741 tgaagttcgt cgtgaaggac ctgaaggaca tcctcaagac ggaaaaaacc gaacgccccg
     1801 cgttcctgct ggcgtacatc ttccacaacg tcggcgacga cgagagcacc gcgaaatatc
     1861 tggatgaagc agaccgccga gtcgccgggc gcgatgggat ggtcaacctg atgcgcaaca
     1921 cgtggggctt gaagaagagc gacacgccgg cgacgccggc gaatccatga gcgggcgcgt
     1981 gtcgtcacgt atccgctttt ggcatcgctc gcgcgcatga attctgatcg cactgttggc
     2041 tttggcatcc ggtggcgcca cgtgtcgctg ttgatcgcca tcgtggtcct tctcgcgctg
     2101 gcagggcggc agtggcattt cgtcgatcaa tactccgtca acatgatgtg ctgggaccaa
     2161 tgggacgact attggcccat gtttcgcggg caggggtggt gggcgtgctt caattttcaa
     2221 cccggcccgc agcggcaggg ggcgggtgag ctgctgatgc gctgcgtcgc cgccgccagc
     2281 gggtggaact cgcgctggga ttcgttctcg atatcagcaa cactgacggc cgcggcgatc
     2341 gccgctatgg cgctcgtgtg gatcgccgac gtgcgcggcg tcgcgctgat cgcgctgccg
     2401 ctgctgttct tcaatgtccg gcagtaccag cagctggtgg ccgacgccaa cgtcgcccac
     2461 ggcgcgatgc cgatcctgct gttgatgttg tacggcctgg cctggtacgc gcggccgatc
     2521 acccttcgcg cggcgctgtt ggtattcctg acgttcctgc tgatcttcac cggatacggc
     2581 atcttcgcgg ggctgctcac gccgatcgtg ttgatcgtcg agctcgcgca ggaaaccgga
     2641 gccggccaac gaagg
//
LOCUS       DATIMH010001199         7853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_1442911, whole genome shotgun sequence.
ACCESSION   DATIMH010001199 DATIMH010000000
VERSION     DATIMH010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442911"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VLI90_20655"
     CDS             complement(<1..706)
                     /locus_tag="VLI90_20655"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HSV16688.1"
                     /translation="MSNLRFNHVFATLMFVGLVSAFVIPPRFTNPARAELQGLLSPIS
                     RPARGIAGIIYRRFHRDVPVDEGSPVNPRPPQTILEENRQLRNALAIMSVKFDQLSRL
                     NEDRQLVGEDIRRLCTPGTVTGSDSSGIRESLTISTAGFGGLKSDMPVVYAHELVGKV
                     ARAGIGGAQVRLITDPGFAFTGRIYRYRTDAEGQVAQERVEGLQPLVQGIGHGAMAIR
                     SNISMAQVKELRISVDD"
     gene            complement(790..1842)
                     /locus_tag="VLI90_20660"
     CDS             complement(790..1842)
                     /locus_tag="VLI90_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSV16689.1"
                     /translation="MFDQLLGMFSVDMGIDLGTCNTLVCIKGEGIVLNEPSVVAVKKG
                     TNRVLQNGQAVGLVAKEMLGKTPGSISAIRPLKDGVISDFEVTEAMLRYFIRRAQGRS
                     HFIHPRVVIAIPSGITAVEKRAVLESAERAGARRVFLVEEPMAAAIGAGLPVTEPTAS
                     MIVDIGGGTTEVAIISLADISVSESVRVAGDDFDEAIVNHMKRTYNMAIGEQMAERIK
                     IEIGSAAALSDDEPNMECRGRDMISGLPRKTTITSQEIREALQEPVTQIIEAVTRTLE
                     LAEPELAADLVDNGITMAGGSSLLRGLTTVISNATGLDVKLAEDPLTCVARGTSVYLE
                     NLELWKDTMESDEDEL"
     gene            complement(1996..2241)
                     /locus_tag="VLI90_20665"
     CDS             complement(1996..2241)
                     /locus_tag="VLI90_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16690.1"
                     /translation="MRHLIATGNLVYYLMNDPGAVIGPWDRAGMREGPDPMERVPREW
                     IRAASIANGLERAAAETAGLGFMSYLTCSRCGLVVTI"
     gene            2407..3171
                     /locus_tag="VLI90_20670"
     CDS             2407..3171
                     /locus_tag="VLI90_20670"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSV16691.1"
                     /translation="MTPSTIAAQLYTLREHTKTPTDIAATLKRVRRIGYQAVQLSALG
                     KIDPKELAKILQGEGLTCCATHISLDRMRDEAPAVIEEHRLWGCSYTAVGGFFPKDPK
                     TQDWLNFARDYNDIARRYTGSGLAIGYHNHSHEMAKFDGQRAMDILLAELDPSIWFEI
                     DTYWIQHGGGDPAAWIKKVAGRIPCVHLKDMGVTNAREPFMMEVGEGNLNWPEILKAC
                     KAAGVRWYIVEQDTCYRDPFDSLETSLKNLQQMDMR"
     gene            complement(3264..3824)
                     /gene="mug"
                     /locus_tag="VLI90_20675"
     CDS             complement(3264..3824)
                     /gene="mug"
                     /locus_tag="VLI90_20675"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HSV16692.1"
                     /translation="MPAPVEAPRKPTPAELIAAIDRTLKDVIAPDLKVLFVGINPGLY
                     SAATGHHFARPGNRFWPALHAGGFTPRVLKPWEERQLPAMGVGITNIIARATATAAEL
                     QAEEFAPGAKALERKVIRYRPKAMAVLGVGAYRLAFGEKTALLGKQDRVIGSTAVWVL
                     PNPSGLNAHYQPKDLARLFRELREAM"
     gene            3826..4395
                     /locus_tag="VLI90_20680"
     CDS             3826..4395
                     /locus_tag="VLI90_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16693.1"
                     /translation="MAGVVADAIRPCYGYRMEARNESGSPAARCPNCDGEIDTTAAEG
                     ACPHCGTALMIIVDDTRQEQTPQTPQRDEELDGLKIRQLIALRRGAIRARSWCLIAAA
                     VCVVAAIQLIAKTVQNVRHTQSWGLRATGFTLFAVGGLMVAGIFLRRAAELKREIDQP
                     TQPAPSKPPEFEALSDGSQRWKNLEDLTD"
     gene            complement(4972..7158)
                     /locus_tag="VLI90_20685"
     CDS             complement(4972..7158)
                     /locus_tag="VLI90_20685"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HSV16694.1"
                     /translation="MRYRSSPFAIFAAMALILVASVTCIYVMGMVPLHRTAELVQRQV
                     VLDHIERVLTTLIDAETGQRGFLLTGDDAYLQPYHDAVARIHGEIDLLRGLAKEGELR
                     QADLDQLLELTNHKLTELDHTIDLRRTQGADAALAVVRSDEGRVTMTALRTHLSVMAD
                     QQLAALRTARATADRFTYYRNLVFGFVTLLNLAFLAWAYGRIQSEMRAQDETAHELRR
                     QKDLTTVTLSSIGDAVIVTDNTVRVTFMNAVAETLTGWKFAEADGQPLTTIFRIINET
                     SRQTVENPVEKVLRLGSIVGLANHTLLIRRDGSEVPIDDSGAPIRDEQGNVHGVVLVF
                     RDFSDYKESERRLTTAKAEAEAASRAKDQFLAALSHELRTPLTPVLATLTNWEGSGRF
                     NAEDQNDITMLRRNVELEARLIDDLLDLTRIAKGKIQLNTEVVDLHDLLRSVKGICQS
                     EINAKHIAAELQLSATRHYVNADSARLQQVFWNILRNAAKFTAEGGHVEICTSDCADG
                     QVCVTIIDNGIGMSQETMDRLFRPFEQGQDEITKRYGGLGLGMSISKALVDVQGGTIA
                     AQSDGAGRGARFIVTLPSVDPPKVSSSSAPERRRSDSGQSLRILLVEDHADTARAMSR
                     LLKSLGHQVETATTVAGALDLAVKNNFRLIISDIGLPDGTGIDFIRQVRRHSNVPAIA
                     LTGFGMEDDIARSREAGFTVHLTKPVNFQKLEMIVQQVANDHAEPG"
     gene            complement(7290..>7853)
                     /locus_tag="VLI90_20690"
     CDS             complement(7290..>7853)
                     /locus_tag="VLI90_20690"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HSV16695.1"
                     /translation="VELVDAADRITAFMPVLDEHLVGGMATLERANVVMYRHGKDDPP
                     GQLQLAGMISPRSTVPKIQTTGTRMRINEDGILLRVFLGESDRFEGKPLHEAIVHKAR
                     ELGLAGATVLRGSEGFGANSVVHKAALLEMSSDLPIVIEIVDTEPRVRQILPHLESMV
                     REGMITMEYVVILMYRHDPAGAPAGGG"
BASE COUNT         1448 a         2563 c         2368 g         1474 t
ORIGIN      
        1 tgtcatccac cgaaatccgc agctccttca cctgggccat tgagatgttg ctgcgaatcg
       61 ccatggcccc gtggccgatg ccctgcacca gcggctgcag gccttcgacg cgctcctgcg
      121 cgacctgtcc ctcggcgtcg gttcgataac gatagattcg accggtgaag gcgaaacctg
      181 gatcggtgat cagccgcacc tgcgccccac cgatgccggc gcgggcaact ttgccgacca
      241 gttcgtgggc gtagacgacc ggcatgtccg acttgaggcc gccgaacccc gcggtgctga
      301 tcgtcaacga ttcacggatg ccggaactgt cggacccggt gaccgtgccc ggcgtgcaca
      361 ggcggcggat gtcttcgccc accaactggc ggtcttcgtt caatcgactg agctggtcga
      421 acttcaccga catgatcgcc agcgcgtttc gaagctgccg gttctcctcc agaatcgtct
      481 gcggcggacg cgggttcacg ggcgagcctt cgtcgaccgg cacatcgcga tgaaagcggc
      541 gatagatgat tccggcgatg ccgcgcgcgg gccgcgagat gggtgagagc agaccctgaa
      601 gttcagcgcg agccggattc gtgaagcgtg gcgggatgac gaaggcgctg acaaggccga
      661 cgaacatcag cgtggcaaag acgtgattga agcgcaggtt agacatccgt gtctgccgta
      721 gcacgggctc tgagcctgtg gctctcccgc ccgtgtctgg aaccgttcgg cacgggcgag
      781 acgcccgtgc tacagttcat cttcgtcgct ctccattgtg tctttccaaa gctcgaggtt
      841 ttccagataa acgctcgtgc cccgggcgac gcaagtgagc gggtcttctg ccagcttcac
      901 atccagcccc gtcgcgttgc tgatgaccgt ggtcaatcca cgcagcagcg acgacccacc
      961 agccatcgtg atgccgttgt ctaccaaatc cgccgccagt tccggctcgg ccagctccag
     1021 cgtgcgcgtc accgcttcga tgatctgcgt caccggttcc tgcagcgctt cgcggatttc
     1081 ctgcgacgtg atcgtcgtct tccgcggcag accgctgatc atgtcgcggc cgcggcattc
     1141 catgttgggt tcgtcgtcgc tcagggcggc ggcggagcca atctcgatct tgatccgctc
     1201 ggccatctgt tcgccgatgg ccatgttgta cgtgcgcttc atgtggttga cgatcgcctc
     1261 gtcgaaatcg tcgccggcca cgcggaccga ttcgctgaca ctgatgtcgg ccagcgaaat
     1321 gatggcgacc tcagtcgtcc cgccgccgat gtccacgatc atgctggcgg tcggttcggt
     1381 cacgggaagc cccgcgccga tcgcggcggc catcggttct tcaaccagaa agacgcgacg
     1441 agcgcccgcg cgttcggcgc tctccagcac cgcgcgcttt tcgacggcgg tgatgcccga
     1501 ggggatcgca atgacgacgc gcggatggat gaagtgcgat cggccctggg cgcggcggat
     1561 gaagtaccgc agcatcgcct cggtcacctc gaagtcgctg atgacgccgt ctttgagcgg
     1621 gcgaatcgca gagatcgatc ccggcgtctt gccgagcatt tcctttgcca ccagccccac
     1681 cgcctggcca ttttgcagaa cgcgattggt cccctttttc acggcgacga cgctcggttc
     1741 gttcagcacg atcccttcgc ccttgatgca cacaagcgtg ttgcaggtgc ccaggtcgat
     1801 gcccatgtcg acgctgaaca tgcccaagag ctggtcgaag atcacgagtg cactccttgc
     1861 gcggccactg aacgaacagc ggttaaagtt ggcaaacttt gcccgtttta ccgctgtggg
     1921 ccgtgtttat aatgcggctg atcgcccggt caaagtccgc gggcgtctaa acgggcgcgc
     1981 ttgccagtcc tagggttaga tcgtcacaac cagaccgcaa cgcgagcagg tgagatacga
     2041 catgaacccc aaaccggcgg tctccgccgc cgcgcgttcg aggccgttcg ctatcgatgc
     2101 ggcgcgaatc cattcgcgcg gcacccgctc catcgggtcc ggaccttccc gcataccagc
     2161 cctgtcccaa ggtccgataa cagcgcccgg gtcattcata aggtaataga cgagattgcc
     2221 cgttgcaatc agatggcgca agaaatgccg attccaagcc gcttgccgct tagcatttgt
     2281 tgttgttctg ttgttcttct gttgctcttc gaatggaaaa gacgctaagc cgcaagcggc
     2341 tggtctatgc cgttgcatgg ggtctggccc acccgtaccc tgtcgcgcat caacaaggga
     2401 accgccatga cgccgtcgac catcgccgct caactttaca cgctgcgcga acacaccaag
     2461 acccccaccg acatcgccgc cacgcttaag cgcgtgcgac gcattgggta tcaagccgtt
     2521 caactttcgg ccttgggcaa gatcgatccg aaggagctgg ccaagatcct gcaaggcgaa
     2581 ggcctcacct gctgcgcgac gcacatctcg ctggatcgaa tgcgcgatga agcccccgcg
     2641 gtgattgagg agcaccggct gtggggctgc tcgtacaccg ccgtcggtgg atttttcccg
     2701 aaggacccta agacacaaga ttggctgaac ttcgcccgcg actacaacga catcgcccgg
     2761 cgatacaccg gcagcggttt ggccatcggt tatcacaatc acagccacga aatggccaag
     2821 ttcgatggcc agcgggcgat ggacatcctg ctggcggaac tggacccgtc gatctggttc
     2881 gagatcgaca cctactggat ccaacacggc ggcggtgacc cggcggcgtg gatcaaaaag
     2941 gtcgccgggc gaattccgtg cgttcacctg aaagacatgg gcgtaaccaa cgcccgcgag
     3001 ccgttcatga tggaagttgg cgaaggcaac ctgaactggc cggagatttt aaaagcctgc
     3061 aaggcggcgg gcgtgcggtg gtatatcgtc gagcaggata cgtgctatcg ggatccgttc
     3121 gatagcctgg agacgagttt gaagaatctg cagcagatgg acatgcgttg agaactgaag
     3181 cgattgacca accgacgccg gtgtctgagg gtacgcccga agacccgggg ggcgtaccct
     3241 cagacaccgc cgtcggttaa tcatcacatc gcctcgcgca gctcacggaa cagccttgcc
     3301 agatccttgg gttggtaatg cgcattaagc ccgctcgggt ttggcagcac ccacaccgcc
     3361 gtcgaaccga tcacgcgatc ctgcttcccc aacagcgcgg tcttctcgcc gaacgctaac
     3421 cgatacgccc ccacgcccag taccgccatc gccttcgggc gataccgaat caccttccgc
     3481 tccaacgcct tcgcgcccgg cgcaaactcc tccgcctgca gttccgcggc tgtcgccgtc
     3541 gcccgcgcga tgatgttcgt gatcccaacc cccatcgccg gtagctgccg ctcttcccac
     3601 ggcttcagca cgcgcggcgt aaacccgccg gcgtgcagcg ctggccagaa gcggttgccc
     3661 gggcgcgcga aatgatgccc ggtggccgcg gaatataagc cggggttgat gccgacgaac
     3721 agcaccttca ggtccggcgc gatgacgtcc ttcagtgtgc gatcgatcgc ggcaatcaat
     3781 tccgcgggcg tgggtttgcg aggcgcttca acaggtgcag gcatcgtggc cggcgttgta
     3841 gccgatgcaa tccgcccgtg ctacggttat cgcatggagg cgcggaacga atccgggtcg
     3901 cccgcagcgc gatgtccaaa ctgcgacggc gagattgaca cgaccgcggc ggaaggcgct
     3961 tgtccacatt gcggcacggc gctgatgatc atcgtcgatg acacgcgcca agaacagacg
     4021 ccacaaacgc cgcagcgcga cgaggagttg gacggcctga agatccgcca gctgatcgcc
     4081 ctccgccgcg gggcgattcg agcgcgatcg tggtgcctca tcgccgccgc cgtctgcgtc
     4141 gtcgcggcga ttcagctgat cgccaagacg gtgcagaacg tccgccacac gcagagctgg
     4201 ggactgcgag cgacgggctt caccttattt gccgtgggcg ggctgatggt cgcgggaatc
     4261 tttcttcgaa gggccgcgga gttgaaacgc gagatcgatc aaccgacgca accggcgccg
     4321 agcaaaccgc ccgaattcga ggcgctcagc gacggcagcc agcgttggaa aaacctggaa
     4381 gacctgacgg actagcgcga ccccctgaca atgtcatcct ggcagcggca cacgactcat
     4441 gcgtcgtcga aggatccagc cgagaagtca gtgaagcatc tctatccgcg ctcaaagccc
     4501 cgcagatacc tgcggggctt ggaacttcga tggatgcctg cgcgagcgga ccgctgcatc
     4561 acatctcatg acgatccttc gcgactgcgg cgaatacgcc tgcgctaaag gatgagatag
     4621 aattgcgcag cgaagcgatc ctcgcgaccc gtgcggatgc cggctgagcc gccgccgccg
     4681 gcagccgcac cgtcgcaatg acaatcacca ctccggaaga aaccgcaaac ccgacactgc
     4741 gcgccggccc cgttcagcag tgttctgcat tgtcgccgac aaggcgggcg cgatggccgt
     4801 gtttgcaaag attgcacgca tgcgcccgga gcgcaaatgc ttggcgaaac ccgaatgacg
     4861 aaatccgaat ccccgaatga aatccgaatg acgaatgacg gaccgcatgc aatcgcgcgc
     4921 aattcgtcat tggtcattcg gatttggggt ttcgtcattc gccaatcacc ctcaccccgg
     4981 ttccgcgtga tcgttcgcca cctgctgcac gatcatctcc agcttttgaa aattcaccgg
     5041 cttggtcagg tgcaccgtga accccgcctc gcgcgacctg gcgatgtcgt cttccatgcc
     5101 gaagccggtc agcgcgatcg ccggcacgtt gctgtgccgg cggacctggc ggatgaagtc
     5161 gatgccggtg ccgtcgggca ggccgatgtc gctgatgatc aaccggaaat tattcttcac
     5221 cgccaggtcg agcgcgccgg cgaccgtcgt cgcggtttca acctggtgac cgaggctctt
     5281 caacaaccgg ctcatcgcgc gggcggtgtc ggcgtggtct tccaccagca ggatgcgcag
     5341 cgattgcccg ctgtcagatc gccggcgctc cggcgcggac gacgatgata ctttgggcgg
     5401 atcgacgctc ggcagcgtga caatgaagcg cgccccacgg ccggcgccgt cgctttgcgc
     5461 cgcgatcgtc ccgccttgca cgtcgaccag cgctttggag atcgacatcc ccaggcccaa
     5521 cccaccgtat cgcttggtga tctcgtcctg tccctgctcg aacggacgga agaggcgatc
     5581 catcgtctcc tgcgacatgc cgatgccgtt gtcgatgatc gtcacgcaga cctggccgtc
     5641 agcgcagtcg ctggtgcaaa tctccacatg cccgccctcg gcggtgaact tggcggcgtt
     5701 gcgcaggatg ttccagaaga cctgctgcag ccgcgccgag tccgcgttca cataatgccg
     5761 cgtcgccgac agctgcagct cggcggcaat gtgcttggcg ttgatctcgc tctggcagat
     5821 gcctttgacc gatcgcagca gatcgtgcag gtccaccact tccgtgttga gctgaatctt
     5881 ccccttggcg atgcgcgtca ggtcgagcag gtcgtcgatg agccgcgctt ccagctcgac
     5941 gttgcggcgg agcatcgtga tgtcgttctg atcttcggcg ttgaaccgcc cggagccctc
     6001 ccagttcgtc agcgtcgcca gtacgggcgt caacggcgtg cgcagctcgt gcgacagcgc
     6061 cgccaggaat tggtccttcg cgcgactcgc ggcttccgcc tcggccttgg cggtggtcaa
     6121 tcggcgctcc gattccttgt agtcgctgaa atcgcggaag acgagcacga cgccgtgcac
     6181 gttgccctgc tcgtcgcgga tcggcgcgcc gctgtcgtcg atcggcacct cgctgccgtc
     6241 gcggcggatc agcagcgtgt gattggccag acctacgatt gagcccagcc gcaaaacctt
     6301 ctcgaccgga ttctccaccg tctgccggct ggtttcgttg atgatgcgga agatcgtcgt
     6361 caacggttgg ccgtcggctt cggcgaattt ccaacccgtg agtgtctccg ccaccgcgtt
     6421 catgaacgtc acgcgcacgg tgttatcggt gacgatgacg gcgtcgccga tgctcgatag
     6481 tgtgacggtc gtcaaatcct tctgccgtcg cagctcgtgc gccgtttcat cctgcgcgcg
     6541 catctccgat tggatgcgcc catatgccca ggcgagaaac gcaaggttca gcagcgtgac
     6601 gaagccgaac acgagattgc ggtaatacgt gaagcgatcg gcggtcgcgc gggccgtccg
     6661 cagcgccgcc agttgctggt cggccatgac gctcaagtgc gtgcgcaacg cggtcatcgt
     6721 cacgcggcct tcatcgctgc gaaccaccgc cagtgctgcg tcggcgccct gggtccgtcg
     6781 caggtcgatc gtgtgatcca gttcagtgag cttgtggttc gtcagctcga gcagctggtc
     6841 gaggtccgcc tgacgcagct cgccttcctt cgccagcccg cgcagcaagt caatctcgcc
     6901 gtggatgcgc gcgaccgcgt cgtgatacgg ctgcagatac gcgtcatcac ccgtcagcag
     6961 aaatccgcgc tggccggttt cggcatcaat taaagttgtg agcacgcgct cgatgtgatc
     7021 gagcacgacc tggcgctgca ccagttcggc ggtgcgatgc agcgggacca tgcccatcac
     7081 gtagatgcag gtcacgctgg cgaccaggat caacgccatc gctgcaaaga tggcgaaggg
     7141 gctgctgcga tagcgcataa acaaatgacc ctttagacgc cgcgactcgc gaaacccgcc
     7201 ctggcgcgcg ccgcccgcgc atgggttcta taagtcgccg cgaatcacgc aacctccctg
     7261 ggaatatacc aaatggaatc cgcgattcgc tacccgccac ccgctggcgc acccgccgga
     7321 tcgtgccgat acatcaggat cacaacatat tccatcgtga tcattccctc gcgcaccatc
     7381 gattccaggt gcggcaggat ctgcctgacc cgcggctcgg tatcgacaat ctcaatcacg
     7441 atcggcaggt ccgatgacat ctccagcagc gccgccttgt gcaccacgct gttggcgccg
     7501 aagccttcgg acccgcgcag caccgtcgcg ccggccaggc ccagctcgcg cgccttgtgc
     7561 acgatcgctt cgtgcagcgg cttaccttca aagcggtcag actcgcccag aaacacgcga
     7621 agcagaatgc cgtcttcgtt aatacgcatg cgcgtccccg tcgtttgaat tttgggaacc
     7681 gtcgatcgcg gcgagatcat cccggccaat tgcagttgcc cgggcgggtc gtctttgccg
     7741 tggcgataca tgacgacgtt cgcccgctcc agcgtcgcca tcccaccgac gagatgttca
     7801 tcgagcaccg gcataaacgc ggtgatgcga tcggcggcat cgacaagttc cac
//
LOCUS       DATIMH010001200         5782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2304929, whole genome shotgun sequence.
ACCESSION   DATIMH010001200 DATIMH010000000
VERSION     DATIMH010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304929"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VLI90_20695"
     CDS             complement(<1..344)
                     /locus_tag="VLI90_20695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16696.1"
                     /translation="MISSPLRAATPQQVDEMLTKAKAYLYNKQRNGNWEVADKAEADD
                     QFYSPGSGQWGGTTALTVYALLAAGESPQDPRLAPAIAFLKKAEIRGVYALSLRCQVW
                     PLLPQSQEIHKLA"
     gene            complement(451..1848)
                     /locus_tag="VLI90_20700"
     CDS             complement(451..1848)
                     /locus_tag="VLI90_20700"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HSV16697.1"
                     /translation="MRTKRLFRFVLVLATIMSFAASARRATAEVKWISSTAASPWQDM
                     AAPAWSAAEDERPPEIRIDPRRTFQTIDGFGGCFNELGWRALSALSDEERSRVMAALF
                     GDDGCAFTLARLPIGASDFAFDWYSLDDTPGDLALEHFSVDRDGNALLPFVKAAMRVR
                     RDLKCWGSAWSPPAWMKTNNQYNGGSIRWEPDVLRAYANYLARWVEAYRAEGVNVFAV
                     FTQNEPNVASKYPSCIWTGDQLRSFIAGDLGPTLRDRKTNVELWLGTLNDGFVNGRAI
                     TVLSDPAANTFVTGVSFQYDARASIASVAHLYPDKKLMQSETECNSGENSWADAQRLY
                     GLMKHYLDRGANSYFMWNMVLDETGLSSWNWRQNAMMTVDRASKTVRFNGEYYVMRHF
                     SEFVKPGAKRAIVSGVWGDQIGFVNPDGSIVLAIGNSSGKAMPVHICFANHADGDTLA
                     VTLPAGSINTFVLAR"
     gene            complement(1970..4513)
                     /locus_tag="VLI90_20705"
     CDS             complement(1970..4513)
                     /locus_tag="VLI90_20705"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HSV16698.1"
                     /translation="MNRRLASCLASVVVLLLSAGAMAVTPARVDIDVNKPGAAIPPSF
                     YGLMTEEINHSYDGGLFAEVIQNRTFQDPLPPMRGRKRPPQDVPADPPIHWSLVQDNG
                     GQASMHTDRTDPVNAALPISLRLDLSGGRAGVANDGYWGIPIRPDTTYTASFYVKSGG
                     GFAGPVTAALITDDGNVTVAKADSEPITAQWKKYTVTLTTAHDAPTTAKARFALLASG
                     SGSVSFSLVSLFPPTYMDTPGGLRADLMKLMADMHPAFIRLPGGNYLEGDTFPTRFNW
                     KQMIGAADQRPGHMGCWSYRSSDGFGLPQYLLWCKQLNAEPVLAVFAGYTLNHDHVDA
                     GPKLQQYVDEALEEIEYVSGPADSTWGKRRAEDGFTEPFALHYLEVGNEDWFDRSGSY
                     DGRFTQFFDAIKSKYPQLKIIATAPVKTRTPDLIDDHYYRSARQMAFDWRHYDRGTEE
                     QNRKYPRNGPQIFVGEWATQEGRPTPDLNAGLADAAWLMGLEHNADLIPIECYAPLLV
                     NVNPEDATKGYPQGWQWPTNLIGYDALNSFGSPSYYAQAMLGQNKGDVVLPVKMTVPP
                     TIAATEPAPHGMVGVGTWHTQVEYKDIVVTAPGGNTLLTADLTKDTNGWHFTGPKWNL
                     QDQVLKPSAADSTSWATIGDPKWTDYDVHVKARKLGGAEGFLVLYHVIDGENFNWWNV
                     GGWGDSKTQLEATHDDARAPYGPEAPFQVETGKWYDLRVEVRGHRARCFVDDKLITDG
                     TDQPQPVATPMFASASYVNGDHTVIVKVVNLGNDPIETTLNLNGVRGVKPEATAIVLS
                     GEPRDVNTIQEPTKVAPKTETLKNASRSFTRNFPPHSLTLLRVTAEAMP"
     gene            4861..>5782
                     /locus_tag="VLI90_20710"
     CDS             4861..>5782
                     /locus_tag="VLI90_20710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HSV16699.1"
                     /translation="MLLTLTTTHSPATDLGFLLHKNPARPQAFVLAFGRAHVLYPEAS
                     EERCTAALILEIDPIGLVRNRRGPAGEGFALQQYVNDRPYVASSFLSVAIAQVFGSGM
                     TGTSRERAALADTPIPLTAKISVLPCRGGEALLRRLFEPLGYQVVATRHPLDEAFPEW
                     GESPSYTVELSHTIRLHELLSHLYVLMPVLDNDKHYWIGDEEVTKLLRHGETWLAAHP
                     EKEQIVERYLKHRRHLTEAALAQLLEEDQQDADDVEEHHASEEERVERKISLNERRLE
                     AVVAALNSAGAKRVLDLGCSYGNLLRRLLTE"
BASE COUNT          988 a         1845 c         1866 g         1083 t
ORIGIN      
        1 gccagcttgt ggatctcctg cgactgcggc agcagcggcc agacctggca gcgcaagctc
       61 agcgcgtaga cgccgcggat ctccgccttt ttcaggaacg cgatcgccgg cgccagccgc
      121 ggatcctgcg ggctctcccc cgcggccagc agcgcgtaca ccgtcagcgc cgtcgtccca
      181 ccccattggc cggaaccggg gctgtaaaac tgatcgtccg cctccgcctt gtcggccacc
      241 tcccaattgc cgtttcgctg cttgttgtaa aggtacgcct tggccttggt gagcatctca
      301 tccacctgct gcggcgtggc cgcgcggagg ggcgatgaga tcacgagaag gaccagcgtc
      361 agaacaacga ggcagattcg ccgggagatt tgtgggcgtt ggcgtttcat ctggcggaga
      421 agaataccgc gccgcggcga ctctcgcgcg tcatcgcgcg aggacaaacg tgttgatcga
      481 acccgccggt agcgtgacgg cgagcgtgtc gccatccgcg tggtttgcaa agcaaatatg
      541 caccggcatc gccttgccgc tggaattgcc gatcgccagc acgatcgatc cgtcgggatt
      601 cacgaagccg atctgatcgc cccacacgcc gctgacgatc gcgcgcttcg cgcccggctt
      661 cacaaactcg ctgaagtggc gcatgacgta atattcgcca ttgaatcgga cggttttgct
      721 cgcgcgatcg accgtcatca tcgcgttctg ccgccaattc cacgagctca ggcccgtctc
      781 gtccagcacc atgttccaca tgaaatagct gttggcgccg cgatcgaggt agtgtttcat
      841 caggccgtac aggcgctgtg cgtcggccca ggaattctcg ccgctgttgc actcggtctc
      901 cgactgcatc agcttcttgt ccggatagag atgggcgacg ctggcgatcg acgcccgggc
      961 gtcgtactga aacgaaacgc cggtgacgaa cgtgttggcg gccgggtcgc tcaacacggt
     1021 gatcgcgcga ccgttgacga agccgtcatt cagcgtgccg agccacagct ccacattcgt
     1081 cttgcggtcg cgcagcgtcg gcccgagatc gcccgcgatg aacgaacgca gctgatcgcc
     1141 ggtccagatg caggatggat atttgctcgc aacgttcggc tcgttctgcg tgaacacggc
     1201 gaagacgttc acgccttctg cgcggtaggc ttcgacccat ctcgccaggt aatttgcgta
     1261 tgcccgcagc acgtccggct cccaccggat cgagccgccg ttgtactggt tgttcgtctt
     1321 catccacgcc ggcggactcc aggcggagcc ccagcatttc agatcgcgcc gcacgcgcat
     1381 cgccgccttc acgaacggca gcagcgcgtt tccatcgcgg tcgaccgaga agtgttcaag
     1441 cgccaaatcc ccaggcgtgt cgtcaaggga gtaccagtcg aacgcgaaat cgctggcgcc
     1501 gatcggcaag cgagcaagcg tgaaggcgca gccgtcgtcg ccgaagagcg cggccatcac
     1561 gcggctacgt tcttcatcag acaacgcgct cagcgcccgc cagcccagtt cgttgaagca
     1621 cccgccgaag ccgtcgatcg tctgaaacgt ccgccgcggg tcaatgcgga tctccggcgg
     1681 ccgctcgtcc tccgcggcgc tccacgccgg cgcggccatg tcctgccaag gactcgcggc
     1741 ggtggagctg atccacttca cctcggccgt cgcgcgccgc gccgaagcgg cgaacgacat
     1801 gatcgtggcg agaaccaaga cgaagcgaaa caaacgtttc gtgcgcatgg tgagcattgt
     1861 gatggagtac ttgatagccc aggccggaag gccggggatc ggtctgcaaa cagcgccatt
     1921 tgcaatccga tctccggcct tccggcctgg gttacgattt cgcgacctct tacggcatgg
     1981 cctccgcggt cacgcgcagc agcgttagcg aatgcggggg gaaattccgc gtgaagcttc
     2041 gcgaggcgtt cttcagcgtc tccgtcttcg gcgccacctt cgtcggttcc tggatcgtgt
     2101 tcacatcgcg cggctcgccg ctgaggacga tcgccgtcgc ctccggtttc acgccgcgca
     2161 cgccgttcag gttcagcgtt gtctcgatcg ggtcgttgcc gaggttgacg accttcacga
     2221 tcaccgtgtg atcgccgttg acgtagctgg cggaggcgaa catcggcgtg gcgacgggct
     2281 gcggttgatc ggtgccgtcg gtgatgagct tatcgtccac gaagcagcgg gcgcggtggc
     2341 cgcggacttc cacgcgcagg tcgtaccact tgccggtctc gacctggaag ggcgcctcag
     2401 gcccgtacgg cgcgcgggcg tcatcgtgcg ttgcttccag ctgcgtcttg gaatcgcccc
     2461 atccaccgac gttccaccag ttgaagttct cgccgtcgat gacgtgatac aggacgagga
     2521 atccctcggc tccgccgagc ttccgcgcct tcacgtgcac gtcatagtcg gtccacttcg
     2581 ggtcgccgat ggtggcccag ctggtgctgt cggcggcgct gggtttgagc acctggtcct
     2641 gcaggttcca cttcgggccg gtgaagtgcc agccgttggt gtccttggtg agatcggccg
     2701 tcagcagcgt gttgccgccg ggcgcggtga cgacgatatc cttgtactcg acctgcgtgt
     2761 gccaggtgcc cacaccgacc atgccgtgcg gcgccggttc ggtggcggcg atcgtcggcg
     2821 ggacggtcat cttcaccggc agcacgacgt cgcccttgtt ctgcccgagc atcgcctgcg
     2881 cgtaatagct gggcgatccg aaggaattga gcgcatcgta gccgatcaag ttagtcggcc
     2941 actgccagcc ctgcggatat cctttggttg catcttctgg attcacatta accagcagcg
     3001 gcgcgtagca ctcgatgggg atcaggtccg cgttgtgctc cagccccatg agccaggccg
     3061 cgtcggccag gccggcgttc aggtccggcg tgggccggcc ttcctgcgtg gcccattcac
     3121 cgacgaagat ctgcgggccg ttgcgcgggt acttgcgatt ctgctcctcc gtcccgcggt
     3181 cgtagtgccg ccagtcgaac gccatctgcc gcgccgaacg gtaatagtgg tcgtcgatca
     3241 aatccggcgt gcgcgtcttc accggcgccg tggcgatgat cttcagctgc gggtacttgc
     3301 tcttgatcgc gtcgaagaac tgcgtgaagc gaccatcgta actgccggac cgatcgaacc
     3361 agtcttcgtt ccccacctcg agataatgca gcgcgaacgg ttcggtaaaa ccgtcttccg
     3421 cgcggcgttt gccccaggtg ctgtcggccg ggccgctgac gtattcgatc tcctccagcg
     3481 cctcatcgac atactgctga agtttcggac cggcatcgac gtgatcgtgg ttcagcgtgt
     3541 aacccgcgaa caccgccagc acgggctcgg cgttcagttg cttgcaccac agcaggtatt
     3601 gcggcagacc aaagccgtcg ctgctgcgat agctccagca tcccatgtgc cccggccgct
     3661 gatcggcggc gccgatcatt tgcttccagt tgaagcgcgt cgggaacgtg tcgccttcga
     3721 ggtagttccc accgggcagg cggatgaacg ccgggtgcat gtcggccatc agcttcatga
     3781 ggtccgcgcg cagaccgcct ggcgtatcca tgtacgtcgg tgggaacaac gagacgagac
     3841 tgaacgacac cgagccgctg ccgctggcga gcagcgcgaa gcgcgccttc gcggtggtcg
     3901 gcgcatcgtg agcggtcgtc agtgtgacgg tgtatttctt ccactgcgcg gtgatcggct
     3961 cactgtccgc cttggccacg gtgacgttgc cgtcatcggt gatcaacgcg gcagtcaccg
     4021 gccctgcgaa accaccgccg gacttgacgt agaagctggc ggtgtacgtg gtgtcgggac
     4081 ggatcggaat tccccagtag ccatcgttgg ccacgccggc gcgcccgccg gagagatcga
     4141 gtcgaaggct gatcggcagc gccgcgttca ccggatcggt gcgatcggtg tgcatcgacg
     4201 cctgtccgcc gttgtcttgg acgagcgacc aatggatcgg cgggtccgcc ggcacgtcct
     4261 gcggcggacg tttgcgccca cgcatcggcg gcagcggatc ctgaaacgtg cggttctgaa
     4321 tcacctcagc gaacagcccg ccgtcgtaac tgtggttgat ctcttcggtc atcaacccgt
     4381 agaagctcgg cgggatggcc gccccgggtt tgttgacgtc gatgtccacg cgcgccggcg
     4441 tcaccgccat cgcgcccgcc gagagcagca gcacgaccac ggacgccaga caggaagcga
     4501 gtcgccgatt catttgaacc tccaccgcga agagatcccg cgccgatgac gcaaacgccc
     4561 agcttaactt gtcgtcggct gttgcagtac atgttccaca atatggagaa attgtccagg
     4621 gcgatgattc ttcgccgagt tcagaaggcg gcgctcaggt gcgcaatgcc acgccgggtt
     4681 cggagcgtac tcgcgacgaa gcgagattct gcggcgcgac tgaagcacag catccgggtc
     4741 gtcggagtcc ctccgaactc ggcgtggact gatttagcgc accgcattgc aaaaacatca
     4801 ggtcgtcatc accgggcaac ggcgagggca atgtggtaag aactcaattc atagacaacg
     4861 atgttgctca cgctcacaac cacgcacagc ccggcgacgg atttgggttt ccttctgcac
     4921 aagaaccccg ctcggccgca agcgttcgtg ctggccttcg gccgcgcgca cgtgctgtat
     4981 ccggaggcga gcgaggagcg ctgcaccgcg gcgctgatct tggagatcga tccgatcggc
     5041 ctggttcgca accgccgtgg gccggcgggt gaggggttcg cgctgcagca gtacgtgaac
     5101 gatcggccgt atgtcgcgtc atcgtttctg agcgtggcga tcgcgcaggt gttcggctcc
     5161 gggatgaccg ggaccagccg tgagcgcgcg gccttggcgg acacgccgat cccgctgacg
     5221 gccaagatca gcgtgctccc ctgccgcggc ggcgaagctc tgctgcggcg actgttcgag
     5281 ccgctgggct atcaagtggt cgcgacgcgg catccgctgg acgaggcgtt tccggaatgg
     5341 ggggaaagcc cgtcttacac ggttgagttg tcgcacacga tccggctgca cgagctgctg
     5401 tcgcacctgt acgtgctgat gccggtgctc gacaacgaca agcactattg gatcggcgac
     5461 gaggaagtga ccaagctgct gcggcacggt gagacctggc tggcggcgca ccccgagaag
     5521 gagcagatcg tcgagcgcta tctgaagcac cgccggcatc tgaccgaggc ggccctggcg
     5581 cagttgctgg aggaagacca gcaggatgcg gacgatgtcg aggagcatca cgcgagcgaa
     5641 gaagagcggg ttgagcgcaa gatcagcttg aacgaacggc ggctggaggc ggtcgtggcg
     5701 gcgttgaact ccgccggtgc caagcgggtg ctggacctgg gatgcagcta cggcaatctg
     5761 ctgcgccggt tgctgacgga gc
//
LOCUS       DATIMH010001201         5877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2885005, whole genome shotgun sequence.
ACCESSION   DATIMH010001201 DATIMH010000000
VERSION     DATIMH010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2885005"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /gene="selB"
                     /locus_tag="VLI90_20715"
     CDS             complement(<1..1136)
                     /gene="selB"
                     /locus_tag="VLI90_20715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HSV16700.1"
                     /translation="MSGANPPIRPYILATAGHVDHGKSALVLALTGIDPDRLPEEKAR
                     GITIELGFAELRLPSPPDVAPAVEYQVGVVDVPGHEDFVKNMVAGVGSIDAAMLVVAA
                     DDGWMPQTEEHLQILTYLGVSHGVIALSKIDLAVDEQRAIDAVRQKLRGSPLADAPII
                     PTSVPAKRGLTELKSALAEVLARTPPQRDLGKPRLSIDRAFSLKGVGTVVTGTLAGGQ
                     LRRGQQVIVQPTGVATRIRGMQSHNREIELALPGSRVALHLADVHPSDGRAMRAPSTV
                     GRGDVVSVAELGAPSRTIDVLLERSSRQATARTLKNGTLVQIHHGTSATPARVRLLGA
                     TQLEPGNRGIARLALQSPLLALTGDRFVVRDWPEQHTLAGGIVL"
     gene            complement(1133..2518)
                     /gene="selA"
                     /locus_tag="VLI90_20720"
     CDS             complement(1133..2518)
                     /gene="selA"
                     /locus_tag="VLI90_20720"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HSV16701.1"
                     /translation="MSRASQRPSLRDIPSVDRILRDLGEQDLPRPAVLAVVRRELGLF
                     RAKPSDPSSLMPRIAAALDHLRLTCIQPVINGTGIIIHTNFGRSPLARVAIDALTAVA
                     ADYNNLEYDLATGNRGNRAAYVEHNLAILCGADAATVVNNCAAALVLILRHFTAKPPR
                     TEVIISRGELVQIGGGFRVPEILEASGATLREIGTTNKTTIDDYRAAIDADRTAMVLR
                     VHRSNFYMGGFVEAPSSREIAELTRRAEVPFVEDLGSGATFDTQSLGGGERETMPAEA
                     IEAGADIICFSGDKLLGGPQAGIIAGHAARITALKGEPFFRALRCDKLILSALQATVD
                     VLLSGRVDEIPIRALMDLSMEQLQSRAQTIAADLAGLRLDVKITPCESQVGGGSLPRT
                     SKASLSIDLAVSVEALDAFAQRLRLGRPPVIGYIAGGRLRFDLRTIFPRQDEAFTAAI
                     RTAAVEAGRSR"
     gene            complement(2515..3513)
                     /gene="selD"
                     /locus_tag="VLI90_20725"
     CDS             complement(2515..3513)
                     /gene="selD"
                     /locus_tag="VLI90_20725"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HSV16702.1"
                     /translation="MSQTLLAQVLRRFPPRARSDDRNVIVGSRTFDDAGIYRLRRDCA
                     LVQTVDFFTPIVDDPFDFGQIAAANAMSDVYAMGGRPLTALNLLAVPSETIAPDVIAK
                     ILRGGARKAAEAGCALVGGHTIRNPEPMYGLSVTGIVHPKRILTNAAARVGDLLVLTK
                     PLGTGIASTAIKRSIAEPSLVTRATNVMKKLNIAGATLAERRLVKAATDVTGFGLLGH
                     LANILRASGVGAELHVRSIPVLHRSIWNLIEQDCIPGGTVANLESIAPITDWSDIPDA
                     ARVLLADAQTSGGLLLCVAPKNLNAVMTILRSHRTPAAAVIGSITRSSRPRILVQP"
     gene            complement(3701..4759)
                     /locus_tag="VLI90_20730"
     CDS             complement(3701..4759)
                     /locus_tag="VLI90_20730"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HSV16703.1"
                     /translation="MTEDGIRNFVDRPRAVRRGVHIAASLVILLLAGSSFAATPTTGP
                     ANRSFRFTYAATITGLTAQQHARVWLPMPPSNAQQQVDVISQQLPVTPKVGQEQKYGN
                     RVLYFEANADDHGEIPLRITYRVTRHECGESDLPDAAGLIDELWLQPDALVPVGGRSL
                     TLLEGKTLPTDSLQLARVLYDVVDDHMEYRKDKPGFGRGDADWACDSGFGNCTDFHSL
                     FISLARANHIPARFEMGFPIAPAGTKGPSPIAGYHCWAWCKPAAHAWVPVDISEANKH
                     PDRRNYFFGHLDADRVAFSIGRDLTLVPPQDGPPLNYFAYPYVEVDRKPHPLEKIMRH
                     FESEEITPVATIPENKNG"
     gene            complement(4756..5370)
                     /locus_tag="VLI90_20735"
     CDS             complement(4756..5370)
                     /locus_tag="VLI90_20735"
                     /inference="COORDINATES: protein motif:HMM:NF039702.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6250 domain-containing protein"
                     /protein_id="HSV16704.1"
                     /translation="MNWSSELESGGTVAAADAQLAIDVPAGCTVWFKPMLSGPVMIEY
                     EATAISAGGANDRVSDLNCFWMARDSRSPNDIFGAKRSGKFSDYDALRTYYVGQGGNG
                     NTTTRFRRYIGESKNRPLLPEHDLRGREDMLTPNVPQTIRLIANGSIVQYYRDGKRVF
                     DFQDPQPYTSGWFALRTTKNHMTVRQFRVYRLTPVPATQPSQKP"
BASE COUNT         1018 a         1806 c         1970 g         1083 t
ORIGIN      
        1 tcgagcacga tgccgccggc gagcgtgtgc tgctcgggcc agtcgcggac gacgaagcgg
       61 tcaccggtca gcgccagcaa cggcgattgc aatgcaaggc gcgcgatgcc gcgattgccc
      121 ggctccagct gagtggcacc aagcaaccgc acgcgtgccg gcgttgcgga cgtgccgtga
      181 tggatttgca cgagcgtgcc attcttgagt gtccgcgccg tcgcctgccg cgatgatcgc
      241 tcaagcagca cgtcgatggt gcggctcggc gctcccagtt ccgcgacgct caccacatcc
      301 ccgcgcccca ccgtgctcgg tgctctcatc gcccggccat cgcttggatg gacgtcagcc
      361 agatgcagcg cgacgcgcga ccctggcagt gccagttcga tctcgcgatt gtgcgactgc
      421 ataccgcgta ttcgcgttgc gacgcccgtc ggctgcacga tcacctgctg cccgcgccgc
      481 agctgcccac ccgctaaggt tccagtaacg acggtgccga cgcctttgag tgagaatgcc
      541 cgatcgattg agagccgcgg cttgcccaaa tcgcgctggg gcggcgttcg cgccagcacc
      601 tccgccagcg ccgacttcag ttcagttaac ccgcgcttgg ccggcaccga tgttgggatg
      661 atcggcgcat ccgccagcgg cgatccacgc aacttttgcc gcacggcgtc aatcgcccgc
      721 tgctcatcga ccgccagatc gatctttgac agtgcgatga cgccgtgcga gacaccgagg
      781 tacgtcagga tctgcaggtg ctcttccgtc tgcggcatcc agccatcgtc ggcggcgacg
      841 accagcatcg ccgcgtcgat cgagccgaca ccggcgacca tgttcttcac gaaatcttcg
      901 tgcccgggca catcgaccac gccgacctga tattccaccg cgggcgcaac gtccggcggc
      961 gacggcaggc gcagctcggc gaagccgagt tcgatcgtga tgccgcgcgc tttttcctcc
     1021 ggcaggcgat cgggatcgat gccggtcagc gccagcacca gcgcgctctt gccgtgatcg
     1081 acatgcccgg cggttgccag gatgtacggg cgaatcgggg ggttagcgcc gctcatcgcg
     1141 accgccccgc ttcaaccgcg gccgtgcgga tggccgcggt gaaggcctcg tcctgccgcg
     1201 gaaagatcgt tcgcagatcg aaacgcagcc gaccgccagc gatgtacccg atcactggcg
     1261 gacgaccgag ccgcaggcgc tgggcgaatg catcgagggc ttcgacgctc acggccaggt
     1321 cgatcgatag cgaagccttc gatgttcgcg gcaatgatcc cccgcccact tgcgattcgc
     1381 acggtgtgat cttcacatcc aaccgaaggc ccgcaagatc ggccgcgatc gtttgggctc
     1441 gactttgcag ttgctccatc gagagatcca tcaacgcgcg aatgggaatc tcatccacgc
     1501 gccctgatag caatacatcg acggtcgcct gcagggccga caggatcaac ttgtcgcaac
     1561 gcagcgcgcg aaagaacggc tcgcccttca gggctgtgat gcgcgctgca tggcccgcga
     1621 tgatgcccgc ctgcgggccg cccagcagtt tgtcgccgct gaagcagatg atgtcggcgc
     1681 ctgcctcgat cgcttcggct ggcatcgttt cgcgctcgcc gccgccgagt gactgtgtgt
     1741 cgaaagttgc cccgctgccc aggtcttcga cgaacggcac ttcggcccgg cgcgtcagct
     1801 cggcgatctc ccggctgctc ggcgcctcga caaacccgcc catgtaaaag ttgctgcggt
     1861 gcacgcgcag caccatggcg gtgcggtcgg catcgatggc ggctcgatag tcgtcgatcg
     1921 tcgtcttgtt ggtcgtgccg atttcgcgca gcgtggcgcc gctcgcctcg agaatctccg
     1981 gcacgcgaaa accgccgccg atttgcacca gttcaccgcg ggaaatgatg acttccgttc
     2041 gcggcggttt ggccgtgaag tgccgaagga tcagcaccag agccgcggcg caattgttga
     2101 cgacggtggc ggcgtcggcg ccgcagagga tcgcgaggtt gtgctcgacg tacgccgccc
     2161 gattgccgcg gttgccggtg gccaggtcat attcgaggtt gttgtagtcg gcggcgacgg
     2221 cggtcagcgc atcgatcgcg acgcgcgcca gcggcgatcg gccgaagttc gtgtggatga
     2281 tgatgccggt gccgttgatg acgggctgaa tgcaggtcag ccgcaggtgg tccagcgcgg
     2341 ccgcgatgcg cggcatgagg ctcgatggat cactcggctt cgcgcgaaac aagcccagct
     2401 cacgccggac gacggcgagc accgcgggac gtggcaggtc ctgctcgcca agatcgcgca
     2461 ggatgcgatc gacgctggga atgtcccgca ggcttggccg ctgcgatgcg cgactcatgg
     2521 ttgcacgagt atacgcgggc gggacgagcg ggtgatagaa ccgatcaccg ccgccgccgg
     2581 ggtgcggtgc gatcgcagga tcgtcatgac cgcgttcagg ttcttcggcg cgacgcacag
     2641 cagcaatcca ccgctggttt gagcgtcggc caacaggacc cgggcggcgt cggggatgtc
     2701 ggaccagtcg gtgatggggg cgatcgactc gaggtttgcg accgtgccgc cggggatgca
     2761 gtcttgctcg atcaggttcc agatcgagcg gtgcaggacc gggatcgatc ggacgtgcag
     2821 ttctgcgccg acgccgctgg cgcgcaggat gttcgccagg tgtccgagca ggccgaagcc
     2881 ggtgacgtcg gtggcggctt tgaccaatcg gcgttcggcc agcgtcgcgc cggcgatatt
     2941 cagttttttc atcacgttgg tcgcgcgggt cacgagcgat ggctctgcga tggatcgttt
     3001 gatggcggtc gaagcgatgc cggtgccgag cggcttggtc agcacaagca aatcgccgac
     3061 gcgcgcagcg gcgttggtga ggatgcgctt gggatgaacg atgccggtga cgctcaggcc
     3121 gtacatcggc tctgggttgc gaatcgtatg accgccgacc agtgcgcagc cggcttcggc
     3181 cgctttgcgc gccccgccgc ggaggatctt tgcaatcacg tctggcgcga ttgtttcgct
     3241 cggcacggcg agcaggttca gcgcggtcag cgggcgcccg cccatggcgt agacgtcgga
     3301 catcgcgttg gcggcggcaa tttggccgaa gtcgaacggg tcatcgacga tgggcgtgaa
     3361 gaaatcgacc gtttggacga gcgcgcagtc gcggcgcagc cggtagatac cggcgtcgtc
     3421 gaaggtgcgc gagccgacga tgacgttgcg gtcgtcgctt cgcgcgcgag ggggaaaccg
     3481 gcgcaggacc tgcgccagga gcgtttggct cagtttggcg gctcaaccgg cgcagctggc
     3541 gagctgcgtc aggcgaatcg cgagatcgtt gcgcttcatg ttcctccttt ctggagcggg
     3601 agtgtagcgg cgccggggtg gggatacacg cacgaatgag cgcgcccgcg cgcgagtgtc
     3661 ggagagagag cagaacttac cgctgaagcg gcaagcccgg tcatccattt ttgttttcgg
     3721 ggatggtcgc gacgggcgtg atctcttcgc tttcgaaatg acgcatgatc ttttcgagcg
     3781 ggtggggttt tcgatcgacc tcgacgtacg ggtaggcgaa gtagttgagc ggcggtccgt
     3841 cctgcggcgg aacgagcgtg agatcgcggc cgatgctgaa ggcgacgcga tcggcgtcga
     3901 ggtggccgaa gaaataattg cggcggtcgg gatgcttgtt ggcttcggaa atgtccaccg
     3961 gcacccatgc gtgagcagcc ggtttgcacc acgcccagca gtgatagccg gcgattggtg
     4021 atgggccttt ggttcccgcg ggcgcgatgg ggaagcccat ctcgaaccgc gcgggaatgt
     4081 gattcgcgcg ggcgagggag atgaagaggc tgtgaaaatc cgtgcagtta ccgaagccgc
     4141 tgtcgcaggc ccagtcggcg tcgccgcggc cgaagccggg cttgtccttc cggtattcca
     4201 tgtggtcatc gacgacgtca tacagcacgc gcgcgagctg cagtgaatcg gtcggcagcg
     4261 ttttgccctc aagcaacgtc agcgatcgac cacccaccgg cacgagagca tccggttgca
     4321 accacagttc gtcgatcaat ccggcggcgt cgggcagatc cgattcacca cattcatgtc
     4381 gagtcacgcg gtatgtgatg cgcaggggaa tttcaccgtg gtcgtctgca ttcgcttcga
     4441 agtagagtac gcggttcccg tacttctgct cttgtcccac cttcggcgtt actggcagct
     4501 gctgcgaaat cacatcgact tgctgttggg cattcgacgg cggcatcggc agccagaccc
     4561 gcgcatgctg ttgggcagtt aaacctgtaa tcgtcgcggc gtatgtaaag cggaacgagc
     4621 gattggccgg tcccgtcgtt ggagtcgcgg cgaatgacga gccggcgaga agtaagatga
     4681 cgagcgacgc agcgatatgc acgccacggc ggaccgctcg cgggcgatcg acaaagttgc
     4741 ggattccatc ttccgtcatg gcttctggct cggctgcgtg gccggcaccg gcgtcaggcg
     4801 atagacgcgg aactggcgca ccgtcatgtg atttttcgtc gttcgcaggg cgaaccaccc
     4861 gctggtgtac ggctgcggat cttgaaaatc gaagacgcgc ttgccgtcgc ggtaatactg
     4921 cacgatcgag ccgttggcga tcaaccgaat cgtctgcggc acgtttggcg ttagcatgtc
     4981 ctcgcggccg cgcaaatcat gctcgggcaa cagcgggcgg ttctttgatt cgccgatgta
     5041 tcgccggaag cgcgtggtcg tgttcccgtt gccgccctgg ccaacgtagt atgtccgaag
     5101 cgcatcgtaa tcggagaatt ttccgcttcg ttttgctccg aaaatatcgt tcgggctccg
     5161 gctgtcccgg gccatccaga agcagttcaa atcgctcacg cgatcgttcg ccccgccggc
     5221 cgaaatggcc gtcgcctcat actcgatcat caccgggccg ctcagcatcg gcttaaacca
     5281 taccgtgcag ccggcgggga catcgatggc cagttgagcg tcggcagcgg ccaccgtgcc
     5341 gccggattcc agttcagatg accagttcaa gaggccacga tcgaaatcgt ctgtgaagag
     5401 attctccgcc acggcaaagc gaggatcggg cgaagcggga agcgtggtcg gcgcagcggc
     5461 tgaaagacgc gctgacgtga acagaagcac gaggaaaacg gagatggctt tcgcggcgaa
     5521 catctcaagc ctcggccaac gacaagcgat cgccgcgcgc tgaatccatt taccaggacg
     5581 tctccgaagc gtaggcatcc taggcttata tcatctgatg ggaagattca ggaacggttt
     5641 gctcaagcaa cgacgcgctg atcgtggcga tgccaccgct cgacacgggc gagacgcccg
     5701 tgctacattt ttggcttgct cgcagatatt cactgccatt gtctgccggg ggtcgatgat
     5761 ggaccggcga cgcttgatga ggcgctgggg ctttgtcgcg cgctggttgc cgacgggatc
     5821 acgacctgcg ttgccacgcc gcatcaactc ggggcgtacg ccggccgcaa tgaagcg
//
LOCUS       DATIMH010001202         5261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4908746, whole genome shotgun sequence.
ACCESSION   DATIMH010001202 DATIMH010000000
VERSION     DATIMH010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4908746"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VLI90_20740"
     CDS             <1..253
                     /locus_tag="VLI90_20740"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSV16705.1"
                     /translation="DIIARDGKTLVFVEVKTRAYDDPQPEDQVNLHKQQQLTKAAKLY
                     LSRYGVPQPPARFDVVAIVWPNGREPQIRHTPNAFDAIS"
     gene            492..2585
                     /locus_tag="VLI90_20745"
     CDS             492..2585
                     /locus_tag="VLI90_20745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16706.1"
                     /translation="MRFICAASVCLALSCLSSFTFAQPAAGVTKPKPDARAGNWLFKT
                     PDPAVWKRGDQAGATTFNVDLPPGDFCTLTLFAGGNADADLIRQFNTAIAEDQKAKGA
                     VKIDGDSGPKPSKSAEGFDVLTRTIVSETNVLHTYNLYITGHSGGRFDMAAFQTSSEE
                     MWKRYGPQASQFLLSLKLANSLDPAEVERLVGKPADTGVQPPALPGFGDAPAADTVPA
                     PAAAPTENVPDVPLDRSPIVKNNAVTDASGKGIDGIKLSQHDTDIGSPSIAVAPDGTI
                     HIAFVEEHRTTYKYAVYHRMSKDGGRTWTEAKNLTEDMPTLGVGKCFVLADAQNRIYV
                     TWRAGIADGFSADVDQRGGRPVDLYFRVLENGKWTKAKRVNEPPDVQRQTDAPMNYYA
                     NVDAAGRVQVIWILPPDKWHPELLYHSQTSVWHANGIGTGLIYQAMLDGANVVHPHEI
                     FLPAIGDNGNAGKFCDGLDIPTGYFDAAGNAHLLANATSITNPVREKSRFELIENGKA
                     GPIIDVPALSFHAWRDIPVLLYDAQGRRHMITLYLAGERPGVRDYLIGSDDEPTVIRA
                     AAAVKGTVDGLQAYQGPGGRMIAIMQMNDTGDRATGETYVSISNGSGWGTPINVTNNA
                     GRRSFASKQTSARSNVAVEKSYYPGPAAAAIDKDGHLLLLMINNEYGLFASTAFGVEM
                     AGGSSSTPMLQFLKF"
     gene            2833..3606
                     /locus_tag="VLI90_20750"
     CDS             2833..3606
                     /locus_tag="VLI90_20750"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HSV16707.1"
                     /translation="MPSSADHFYVDPAFQPLVREIGLDAEAVFSDPRIKPWRVLSDRQ
                     NCTLDETLHDGSTIRLHIKRYPRPEPAATEVAGYRLLAENNIPAASIVAHGRLADGRS
                     FVILPDLTDYTPGDVLVEDQGVPFETLLIATADLAAQLHNANLHHRDLYLCHFMVKIE
                     PATLDVRLIDTARVARLTNPLMRKRWIVKDLAQFWYSTMKLPATDEQRERWLARYAQR
                     RGIEAKTLRSAIDHKVAAIGRHDLRLTRREPRRNISIPR"
     gene            3882..4976
                     /locus_tag="VLI90_20755"
     CDS             3882..4976
                     /locus_tag="VLI90_20755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSV16708.1"
                     /translation="MKIALVILHADPARGGAERYTLDLAAALPRRGHAVSLVASSFAS
                     PAAGNVRQVTLPTSAFTRAGRYRQFLDALDTHLAAEPYDIIHAMLPVRRCDVYHPHAG
                     LALAAAERTGSNLLFNPRRRLMSNVEQALLNAPRPPIVVSLSEYVKREIVQHYPQLPA
                     ERLRTLFNAVDLDRFKPMPPTGVIRSRISGGGLVALFIGHDYVRKGLAEAVAATAKLD
                     EDAPALAVLGPMEWRRYELTPQRHTRVMQFPAVDDPRPYYADADFFVLPTKHDPCSLV
                     VLEALAMGLPVISTRFNGATEIMTDGVQGFIQQDPSDVDALAESMRKMLDPARRSAMK
                     EACLTLRPALSYEHHLDTLEQIYAAARPAS"
BASE COUNT         1013 a         1619 c         1695 g          934 t
ORIGIN      
        1 cgacatcatc gcccgcgatg gcaaaacact cgttttcgtt gaggtgaaaa cgcgggcgta
       61 cgatgatccg cagccggaag accaggtgaa tttgcacaag cagcagcagc taaccaaggc
      121 ggccaagctg tacctgagcc gctacggcgt cccgcaacca ccggcgcggt tcgacgtcgt
      181 ggcgatcgtc tggccgaacg gccgcgagcc ccagatccgc cacacgccaa acgcgttcga
      241 cgcgatcagc tagcgcgccc catcccacta cgccgggttc ggaggtactg cgatgacccg
      301 gattcttctc gcgcgccgca gcgtccgggt cgtcgcgagt acgctccgaa cgcggcgtgg
      361 ggacagtcaa gtcggttttg cgcgtgcaga aattctcagc ggtcgataga gtatccgcgt
      421 cgggtgccgg cgccgctcag gggcgtgcgc aggcgctgca aaaatcaaat cgaacgggag
      481 atcctcacat catgcgattc atttgtgccg cgagcgtttg tctcgcgttg tcgtgcttgt
      541 cgtcattcac cttcgcacaa ccagccgccg gcgtgactaa gcccaagccc gatgcgcggg
      601 cggggaactg gctcttcaag acgccggacc ccgcggtgtg gaagcgcggt gaccaagccg
      661 gcgcgaccac attcaacgtc gatctgccgc cgggggattt ttgtacgctg actttatttg
      721 ccggcggaaa tgccgacgcg gatttaatca ggcagttcaa caccgccatc gccgaggatc
      781 agaaggcgaa gggcgcggtc aagatcgacg gcgacagcgg gcctaagccg agcaagtcag
      841 ccgaaggttt tgatgtgctc acgcggacga tcgtcagcga gaccaacgtg ctgcacacgt
      901 acaacctgta catcaccggc cacagcggtg ggcggttcga catggccgcg ttccagacca
      961 gcagcgaaga gatgtggaag cgctacggcc cgcaggcgag ccagtttttg ctaagcctta
     1021 agcttgctaa ttcgctcgat cccgccgagg tggaacgcct ggtcggcaag ccagccgaca
     1081 cgggcgtgca accgccggcg ctgcccggat tcggtgatgc ccccgcggcg gataccgtgc
     1141 ccgcgcccgc ggcagcgccg acggaaaacg tgccggatgt gccgctcgat cgatcgccga
     1201 tcgtgaagaa caacgccgtc accgatgcca gcggcaaagg catcgacggc attaagctgt
     1261 cgcagcacga caccgacatc ggcagcccgt cgatcgccgt cgcgccggat ggcacgattc
     1321 acatcgcatt cgtggaagag caccgcacga cgtacaagta cgcggtctac caccgcatga
     1381 gcaaagacgg cgggcggacc tggaccgagg cgaagaattt aaccgaagac atgccgacgc
     1441 tcggcgtggg caagtgcttc gtgctggccg atgcgcagaa tcgcatctac gtcacctggc
     1501 gcgccggcat cgccgatggt ttttcggccg acgtcgatca acgcggcggt cggccggtcg
     1561 atctttattt ccgagtgctc gagaacggca agtggaccaa ggcgaagcgc gtcaacgaac
     1621 cgcccgacgt gcagcggcag acggatgcgc cgatgaacta ttacgccaac gtcgacgccg
     1681 ccgggcgggt gcaggtgatc tggatcctgc cgccggacaa atggcatccc gagttgcttt
     1741 atcacagcca gaccagcgtc tggcacgcca atggcatcgg caccgggttg atctatcagg
     1801 cgatgctcga tggtgccaac gttgttcatc cgcacgagat attcctgccg gcgatcggcg
     1861 acaacggcaa cgccgggaaa ttctgcgatg ggctcgatat tcccaccggt tatttcgatg
     1921 ccgccggcaa cgcacacctg ctggcgaacg cgaccagcat cacgaatcca gtgcgcgaaa
     1981 agtcgcggtt tgaattgatc gagaacggca aggccgggcc gatcatcgat gtccctgcgc
     2041 tgtcgttcca cgcgtggcgc gacattcccg tgctgcttta cgatgcgcag ggccggcgtc
     2101 acatgatcac gctgtacctg gcgggcgaac ggccgggcgt gcgcgattat ctgatcggct
     2161 ccgatgacga gcccaccgtc atccgcgccg cggccgccgt caagggtacg gtggacggct
     2221 tgcaggcgta ccagggaccg ggcgggcgga tgatcgcaat catgcagatg aacgacacgg
     2281 gcgaccgcgc caccggcgag acgtacgtct cgatcagcaa cggcagcggc tggggcacgc
     2341 cgatcaacgt gaccaacaac gccggccggc gcagctttgc cagcaagcaa accagcgcgc
     2401 gttcgaatgt tgctgttgag aagagctatt accccggccc cgctgcggcg gcgatcgaca
     2461 aggatgggca cctgctgctg ctgatgatca acaacgaata cggcctgttc gccagcaccg
     2521 ccttcggcgt cgaaatggcc ggcggcagtt cgagcacgcc gatgctgcag tttttgaagt
     2581 tctaaagata agaaattgaa ccacgaagag cacgaagggc acgaagtgga tgcagggttg
     2641 gaaataagcg cgtggtgaat ttcaaccatt ggcgcacggg cctccggccc gtgcgagcga
     2701 tatttgccct tgaatcactt ccgctgccgg atcttcatct gtgtgcatct gtgttgatcc
     2761 gtggctaaaa ctcttgcctt cgtgcccttc gtgccttcgt ggttcaatgg ttctgcccta
     2821 agattatgct gaatgcccag cagcgcagat cacttctacg tcgaccccgc gtttcaaccg
     2881 ctcgtgcgcg agattgggct ggacgcggag gcggttttta gcgacccgcg gattaagccg
     2941 tggcgggtgc tcagcgatcg ccagaactgc acgctcgacg aaacgctgca cgatggatcg
     3001 acgatccgcc tgcacatcaa gcgttacccg cggccggaac cggcggcgac ggaagttgcg
     3061 ggatatcgac tgctggcgga gaacaacatc cccgcggcgt cgatcgtcgc gcatggccgg
     3121 ctggccgacg ggcggtcgtt cgtcatcctc ccggatttaa ccgactacac gccgggcgat
     3181 gtactggtgg aagaccaggg cgtgccgttc gagacgctgc tgatcgccac ggccgatctg
     3241 gccgcgcaat tacacaacgc gaacttgcac catcgcgatc tgtacctgtg tcacttcatg
     3301 gtgaagatcg aaccggcgac gctggacgtg cggctgatcg acactgcgcg ggtcgctcgg
     3361 ctcaccaacc cgctgatgcg aaagcggtgg atcgtgaagg atctcgccca attctggtac
     3421 agcacgatga agctgccggc gacggacgag caacgcgagc gatggctggc gagatacgcg
     3481 cagcggcggg gcatcgaagc caaaacgctg cgcagcgcga tcgaccacaa agtcgcggcg
     3541 atcgggcggc atgatctgcg cctgacgcgg cgcgagcctc gccgcaacat ttccattccg
     3601 cggtgagggg aacgcgaccg cgaagccacg aagacgcgaa gacagacgcg aagggaaaag
     3661 gcgggattgg ggaggaaatg ttcttcgacg acgacaccgc tcgcacgggc cggagacccg
     3721 tgtcacagtg ggcgttcaga agcgttcatc gtgacacggg cctccggccc atgcgcggtg
     3781 cgccggaatt ttaagcactc tcatttcccg ttttctcttc cctttgcgtc ttcgcggtcg
     3841 gatttgtaca tcgccacggt gacgcgtacg ctgcgcgatg attgaagatc gcgctggtca
     3901 tcctccacgc tgatcccgct cgcggcgggg cggagcgtta tacgctggac ctggcggcgg
     3961 cgctgccgcg ccgcggccat gcggtttcgc tggtcgcgtc gtcgttcgca tcgccggcgg
     4021 cgggcaacgt gcggcaggta actctcccca ccagcgcctt cactcgcgcc ggtcgttacc
     4081 gccaattcct cgacgcactc gacacgcatc ttgccgccga gccgtacgac atcatccacg
     4141 cgatgctgcc ggtgcggcgg tgcgatgtgt atcacccgca cgcggggctg gcgctggcgg
     4201 cggccgagcg cacggggtcg aacctgctgt tcaacccgcg gcggcggttg atgtcgaacg
     4261 tcgagcaggc gctgctcaac gcgccgcgcc cgccgatcgt tgttagcttg tccgaatatg
     4321 tgaagcgcga gatcgtgcag cattacccgc agttgccagc ggagcgattg cggacgctgt
     4381 tcaacgcggt ggatctcgat cgcttcaaac cgatgccacc gacgggggtc attcgcagtc
     4441 gaatcagtgg cgggggactg gtcgcgctgt tcatcgggca tgactatgtt cgcaagggat
     4501 tggccgaagc cgtcgccgcc acggcgaagc tggatgaaga cgcgccagcg ctggcggtct
     4561 tgggcccgat ggagtggcgg cggtacgagt tgacgccgca gcgtcacacg cgggtcatgc
     4621 agtttcccgc ggtagacgac ccacggccgt actacgcgga tgcggatttc tttgtgctgc
     4681 cgacgaagca cgatccatgc agcctggttg tcttggaagc tctggcgatg ggcctgccgg
     4741 tcatcagcac gcgcttcaac ggggcgaccg agatcatgac cgatggcgtg caggggttca
     4801 tccagcagga tcccagcgac gtggatgccc tggcggaatc gatgcggaag atgctcgacc
     4861 cggcgcggcg atcggcgatg aaagaagctt gcctgaccct tcgcccggcg ctgtcttacg
     4921 agcatcatct cgacacgctg gagcagatct atgccgccgc gcggccggcc agctaaggtc
     4981 cggataactg tggactgccg cttaccgaaa actgccgaaa cgcgttcgtg aagcgccacg
     5041 gccgatccat gggtcacatc cttcccagct tcacctgatg ccataatatc ggactggcgc
     5101 gagacttagt ttccggttgg cgaggcgcga tccagatgat aggtttcgca cgtcgcgatc
     5161 aagtcgcgcg cggtgaagtg ggtgtgtggg tgtgagttac tctcgggtgc gttgtcgttc
     5221 ggcgcacatg agccctggtc gtagaagggc ggttgatatg c
//
LOCUS       DATIMH010001203         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_283345, whole genome shotgun sequence.
ACCESSION   DATIMH010001203 DATIMH010000000
VERSION     DATIMH010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283345"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VLI90_20760"
     CDS             complement(<1..743)
                     /locus_tag="VLI90_20760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16709.1"
                     /translation="MIFNSLTFLVFFAIVMALHYAPFFSWHQKKINLMIASYLFYAAW
                     NPPFVILLWVSTVVDWWAAQWMVRAKREGTRKAWMLTSVIVNLGMLGYFKYGGFLVQN
                     FVAIAASVGVVYHPPGWDIILPVGISFYTFATLSYTLDVYLRRSKPAGSFLNYALFVT
                     FFPHLVAGPIMRPTELVPQFDQPRQAKPNQIGFGLALLTLGLFQKVVLADGFLAPVVE
                     KVYDAHGQLPGMLDSWVATLAFAGQIFCD"
     gene            complement(894..1652)
                     /locus_tag="VLI90_20765"
     CDS             complement(894..1652)
                     /locus_tag="VLI90_20765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HSV16710.1"
                     /translation="MAGYQGLALLGWVILLASLEVWSGTGSEGASDRRLLTNFSLTLL
                     VLGAGALFPIARLAASTAAQSLNIGLLRSAHWPWTALFAVALLAETFAAYWAHRITHK
                     TPLLWRLHRVHHADNAVDISTSFRNHPLELIITLPVSAAVVLILGTPASVVVATQTLF
                     MGVTLWQHADIALPLHIDRALGWIIVTPRLHRLHHNPTRITNDSNYGELLTLWDRLFG
                     TFNRMEGRRPVGLEGQVASPDRLLQQIWSPVYST"
     gene            1743..1907
                     /locus_tag="VLI90_20770"
     CDS             1743..1907
                     /locus_tag="VLI90_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16711.1"
                     /translation="MRRLENLAFGLALVACASPAFAGQPVPAPIAGVGVGAVVLVGIG
                     YRALKSRINR"
BASE COUNT          451 a          682 c          697 g          384 t
ORIGIN      
        1 aagtcgcaga agatctggcc ggcgaaggca agcgtcgcca cccatgaatc gagcatgccg
       61 ggcagctggc catgcgcgtc gtaaactttc tccaccaccg gggcgagaaa gccgtcggcg
      121 agcacgacct tctggaacag cccaagcgtc aatagcgcca gcccgaagcc gatctggttg
      181 ggttttgcct ggcgcggctg gtcgaactgc gggacgagct cggtcgggcg catgatcggc
      241 cccgccacca gatgcgggaa gaaggtcacg aacaacgcgt agttgaggaa gctgcccgcc
      301 ggcttcgagc gcctgagata gacgtcgagc gtgtaggaca gggtcgcgaa ggtatagaag
      361 ctgatgccga cgggcaggat gatgtcccag cccggcgggt ggtaaacgac gccgaccgat
      421 gccgcgatcg ccacgaaatt ctggaccaga aacccgccgt acttgaagta gccgagcatc
      481 ccgaggttca cgatcacgct ggtcagcatc cacgccttgc gcgtgccctc gcgcttggcg
      541 cggaccatcc attgcgccgc ccaccagtcg accacggtcg agacccacag caggatcacg
      601 aacggcgggt tccacgcggc gtagaacagg taagacgcaa tcatcagatt gatcttcttc
      661 tgatgccagc tgaagaacgg cgcgtagtga agcgccatca cgatggcgaa aaagaccagg
      721 aaggtcagcg aattgaagat catcggacgc cgctccgccg tccggttgca ctatcgtcca
      781 tacagcacgc ccccgagccg cacgcataca ggccgccgaa gactcagcgg cggatcacgg
      841 cgacgttaca cgctggttaa cctggcgcaa gtgaatgcgc cgattggcac cgatcaggtg
      901 ctgtagacgg gcgaccagat ctgctggagc aggcggtcag gcgacgcgac ctggccttcg
      961 agccctacgg gccgccgccc ttccatcctg ttgaaagtgc cgaacaaccg gtcccacaga
     1021 gtcagcagct cgccataatt gctgtcgttg gtgatgcgcg tcgggttgtg atgcaggcga
     1081 tggagccgcg gcgtgacgat gatccagccg agtgcgcggt cgatgtgcag tggcagcgca
     1141 atgtcggcat gctgccacag ggtcacgccc atgaacaatg tctgcgtggc caccaccacc
     1201 gatgccggcg taccgaggat cagcacgacc gccgctgaca ccggcaacgt gatgatcagc
     1261 tccagcggat ggttgcgaaa gctcgtcgag atgtcgaccg cattgtcggc gtggtgcact
     1321 cggtgaaggc gccacagcag cggcgtctta tgggtgatcc gatgcgccca ataagcggcg
     1381 aaggtctcgg ccagcagcgc cacggcaaag agtgcagtcc aaggccaatg cgccgaacgc
     1441 aggagcccga tgttcagcga ctgcgcggcc gtcgacgcgg cgagcctcgc aatcgggaat
     1501 aaagcgccgg ctccgagcac cagcagcgtc aagctgaagt tggtgaggag gcgccggtcg
     1561 cttgcgcctt ccgagcctgt gcccgaccag acctccaaag acgcaaggag gattacccaa
     1621 cccagcaagg cgagaccctg gtagccggcc aactaacctc ctcaaggccg cttgctgcgg
     1681 cccacccatc tgttatattc tcgttaaccg gcttcgtctg caagccttca tgggagagtc
     1741 ctatgcgtcg tctcgaaaat cttgcattcg gtcttgcgct tgtcgcctgc gccagcccgg
     1801 catttgccgg acaacctgtc ccggcgccaa tcgccggcgt cggcgtcggc gccgtcgttc
     1861 tggtcggaat cggctatcga gcgctgaaga gccggatcaa tcgctagcag tcgcttcgac
     1921 cggtaatccg gcggtgaaca gttattttga cgggggttac aatgaataga ttcgagagaa
     1981 ttgcagttgg tgtcgcgctg ctcgctatgg caagtccggc tttcgcaacg tcggtcgacg
     2041 tccctgcacc catcgccggg gtcggcgttg gagcggtact gctgatcggc gcaggttatc
     2101 gggcgatcaa agcacggatc aaataatgtc gttcgttcgg ccggttggaa ttgttccaac
     2161 cgcgccggcc gctttccgga tcaaattcct gcgcggcagc ctattcgctg ccgc
//
LOCUS       DATIMH010001204         4346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4331173, whole genome shotgun sequence.
ACCESSION   DATIMH010001204 DATIMH010000000
VERSION     DATIMH010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4331173"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="VLI90_20775"
     CDS             complement(<1..1580)
                     /locus_tag="VLI90_20775"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSV16712.1"
                     /translation="MSSSTDALLEDLTDAQRQAVTHVDGPLLIVAAAGSGKTRVITRR
                     VAYLIAQGIPPNSILAITFTNKAAGEMKHRIAQVVSRPIHDFGRLDQAWPLICTFHSL
                     CLRILRHYATEIGLPGNFSIFDSADQTKLIKEALKVLDVSSTNFSPGTIHGTISNAKN
                     QLQTPEAFEQSAHDFYARTVARIYKKYQALLNANNALDFDDLLLRTTLAMRDHPQLLR
                     ELQDRFQYILIDEYQDTNHAQYVLAHALALRHRNICVVGDPDQSIYAWRGADIKNILD
                     FEKDYPDARVVKLEQNYRSTKTILQIAGSLIAHNLQRKDKALWTENGQGEKAKLFLCQ
                     DEYDEANIVAAQLKEAHEKHGFRWDQMAIFYRMNALSRVMEDALRKENLPYVMARGTE
                     FYNRKEIKDVLAYLRVIANPQDELSLARIVNVPARGLGDTSVKQMQAHAIATGKTLFA
                     AMQESASITGLSTRAANSVRAFVESVTRWREMAAGARVSEDLLAEQKGPVQLLMEDVV
                     RRSGLEAHYKKEGGDEQA"
     gene            complement(1733..2107)
                     /locus_tag="VLI90_20780"
     CDS             complement(1733..2107)
                     /locus_tag="VLI90_20780"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HSV16713.1"
                     /translation="MIKVKHLLDEAEADDGQRIWVEPIGLTLDLREWCRLDHVLSHLG
                     PPRELWMWFEDHPQAYDYFRGQYHDALSRSPYRTALIDLVSASRRANFTLVHQGDDPE
                     QNTAIALYEFLSELEAYVPPET"
     gene            complement(2819..3091)
                     /locus_tag="VLI90_20785"
     CDS             complement(2819..3091)
                     /locus_tag="VLI90_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16714.1"
                     /translation="MTVRLELPADVEQRLLAEVRAGRHGSIEEAILEKLSRDEDVELM
                     GAMGMDAAQIRSDLDEAWANREGAVDGQTVFARIAAKSASCRAQGK"
     gene            complement(3164..4234)
                     /locus_tag="VLI90_20790"
     CDS             complement(3164..4234)
                     /locus_tag="VLI90_20790"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HSV16715.1"
                     /translation="MEFIQPTIKGRGAAGDPPNRFERIAFAPDGNALHEGGAPGGQRA
                     TVYLKDATRSIITRHDSPDVMIEASINPYRGCEHGCIYCYARPTHEWLGFSAGLDFET
                     KIMVKADAPDLLRSELISPKWQPKMLAISGVTDCYQPIERKLQLTRRCLAVLAEFRNP
                     VGIVTKNHLVTRDIDLLKELASVNAAAVFVSITTLDPQLTRLMEPRTSVPARRLATIE
                     ELANAGIPVGVLMAPVVPGLTDHEIPAVVNSAKNAGAVTAGYVPLRLPYGVAPLFEDW
                     LARHFPDRKDKVLNRIRSMRGGKLNDSNFNTRMKGAGPFADQIAAMFEMAKKRTGLTD
                     RGPSLSIAAFRRPEGPQMELWR"
BASE COUNT          787 a         1354 c         1313 g          892 t
ORIGIN      
        1 tccgcctgct cgtcgccgcc ttccttcttg tagtgtgcct ccagcccgct gcggcggacg
       61 acgtcttcca tcaacagctg cactggcccc ttttgctcgg ccagcaaatc ttcagacacg
      121 cgtgcccccg ccgccatctc gcgccagcgc gtgaccgatt cgacgaatgc gcggaccgaa
      181 ttggcagcgc gcgtcgagag cccggtgatg ctcgccgact cctgcatcgc cgcgaacagc
      241 gtcttgcccg tggcgatcgc gtgcgcctgc atctgcttga cgctcgtgtc acccagcccg
      301 cgcgccggca cgttgacgat ccgcgccagg ctgagctcat cctgcgggtt ggcgatcacg
      361 cgcaggtacg ccagcacatc cttgatttcc ttgcggttgt agaactcggt gccgcgggcc
      421 atcacatacg gcaggttttc cttgcgtagc gcatcttcca tcacgcgcga cagcgcgttc
      481 atgcgataga agatcgccat ctggtcccag cgaaagccgt gcttttcgtg cgcttccttc
      541 aactgggccg cgacgatgtt cgcctcgtcg tattcgtcct ggcacaaaaa cagtttcgcc
      601 ttctcgccct ggccgttctc ggtccacagc gccttgtcct tgcgctgcag gttgtgcgcg
      661 attaagctgc cagcgatctg caggatcgtc ttggtcgatc gataattctg ctccagcttc
      721 accacccgcg cgtcgggata atctttctcg aagtccagga tgttcttgat gtccgcgccg
      781 cgccacgcgt agatggattg atccggatcg ccgacgacgc agatgttgcg gtgccgcagc
      841 gccagcgcgt gggccagcac gtactgggcg tggttcgtgt cctggtattc atcgatcagt
      901 atgtactgga atcggtcctg caattcgcga agcaattgcg ggtgatcgcg catcgccagc
      961 gtcgtgcgca gcagcaggtc gtcgaagtcc agcgcgttgt tcgcattcag cagagcctgg
     1021 tatttcttgt agatccgcgc caccgtgcgg gcgtaaaaat cgtgagcgga ttgctcgaac
     1081 gcctccggcg tctgcagctg gttcttggcg ttgctgatcg tgccgtggat cgtgccgggc
     1141 gaaaagtttg tgctggaaac gtccagtacc ttcagcgctt ccttgatcag cttcgtctgg
     1201 tcggcgctgt cgaagatgct gaagttcccg ggcaggccga tctcggtagc gtagtgccgc
     1261 aggatgcgca ggcacaggct atggaacgta caaataagcg gccaagcttg atcgagccga
     1321 ccgaagtcat ggatcggccg gctgacgacc tgggcgatgc ggtgcttcat ctcccccgcc
     1381 gccttgttgg taaacgtgat ggcgaggatc gagttgggcg gaatgccctg cgcgatcaga
     1441 tacgcgacgc gccgcgtgat gacgcgcgtc ttcccgctgc ccgcggccgc cacgatcagc
     1501 agcggcccgt ccacatgggt gaccgcctga cgctgtgcgt cggtcaaatc ttccagcaat
     1561 gcatcggtcg atgaagacaa accaaaagct cctgtagccg cgacgcctgc gtcgcctttc
     1621 gctgcgcact atagcccgac tcgcgcgcat gaaaaagcga gcggcgacgc tcgatagcat
     1681 cgccgctcgc gtatagcgcg gatcacgtca ctcttgtcga agttatcgcg gttcacgtct
     1741 ccggcggaac gtacgcctcg agttcgctca aaaattcata cagcgcgatc gccgtgttct
     1801 gctcggggtc gtcaccctga tgcaccagcg taaagttcgc ccgccggctg gcggacacca
     1861 agtcgatcag cgccgtgcga tacgggctgc gcgacagcgc gtcgtgatat tgcccgcgga
     1921 aatagtcgta cgcctgcgga tgatcctcga accacatcca cagttcccgc ggcgggccga
     1981 gatggctcaa cacgtgatcg agccggcacc attcccgcag gtccaacgtc aacccgatcg
     2041 gttcgaccca gatccgctgc ccatcatccg cttcggcctc gtccagaaga tgtttcacct
     2101 tgatcacagg tatcgctcct cgtcctgccc atcatccctg tgttcaacat tcaatacgca
     2161 tacgaagcaa acatcattcc caataattga acgcgggaaa gattgtgaag ttcagcacga
     2221 caagggattt cctgaggaag ctgttagtaa ttcataatcc accggttacc accggttatt
     2281 gaccaaggca gagtttggaa gcgatcggct gtgggcagcc cctgatggga cggtcacgcc
     2341 acgcggcatc gcgttggcgg acgtactaag ggcggagtca ctgaaaacga gaaccgggtg
     2401 ccacgtgccc taccagactc gacgcaacgt ccgcgctatg agcttgtgac ggcagttttc
     2461 gttgtacgtg atcagccgga ccgaattcaa ggacgatcgt cgccgccgcg ggaaacccct
     2521 cactcattgt cgctgtgcat ccgcaattga acgtcttggg cggcgtgata aattcgcgcg
     2581 atgtatagcg gcgacgaggc ggggtcgtaa atcaggaaca tctaaacacg atagaagcgc
     2641 aacggccggt ccgtcaggtc cgcacgagag tggcccaggt gtggttgctc gattagtcgg
     2701 ccaatcgcgt cgcgaatttc agccaatatg cggtcggcca gtcgcgttcc gccgcgattg
     2761 aaataatagt cccacaaatc atttaacgca tgctgcgctt gagatgaaag ggtgaagctc
     2821 acttgccttg agcccggcaa gatgcgctct tggcggcgat gcgcgcgaag accgtttgac
     2881 cgtctacggc gccttcacga ttggcccatg cctcatccag atcgcttcgg atttgcgctg
     2941 catccatgcc cattgcgccc atgagttcca cgtcttcatc gcggctaagt ttctccagaa
     3001 tggcctcttc gatagagccg tgacgacctg cgcgcacttc cgcgagcagt cgctgttcca
     3061 catccgcggg taattcaagt cgtaccgtca tggcaacatt gtatgatggg cggcgctggc
     3121 gaagcaaaac aatctgcgtg ctgccaagaa atcttcgcac gtgctaccgc cacaattcca
     3181 tctgcggccc ttcgggccgg cggaaggcgg cgatcgacag gctcgggccg cgatcagtca
     3241 aaccggtgcg cttcttcgcc atctcgaaca ttgccgcgat ctgatcggcg aaggggcccg
     3301 cgcctttcat gcgcgtgttg aagttcgagt cgttcagctt gccgccgcgc atcgagcgga
     3361 tgcggttcag gactttgtcc ttgcgatcgg gaaagtgtcg cgccagccag tcttcgaaca
     3421 gcggcgcgac gccgtacggc aggcggagcg ggacgtaacc agccgtgacc gcgccggcat
     3481 ttttggcgga attcacaacc gcggggatct cgtgatccgt caaccccggc accaccggcg
     3541 ccatcagcac accaacggga attccggcgt tggccagctc ctcgattgtc gcgagccggc
     3601 gcgccggcac gctcgtgcgc ggctccatca gcctcgtcaa ctgcgggtcc agcgtcgtga
     3661 tcgacacgaa caccgcggcg gcgttcaccg atgccagctc cttcagcaga tcgatgtcgc
     3721 gcgtgaccag gtggttcttg gtgacgatgc cgacggggtt gcggaattca gccagcaccg
     3781 ccaggcaccg gcgggtcagc tgcagcttgc gttcgatcgg ttgatagcaa tcggtgacgc
     3841 cgctgatggc cagcatcttc ggctgccact ttggtgatat taactccgat cgcaatagat
     3901 ccggcgcatc ggccttcacc atgatcttgg tctcgaagtc cagccccgcg gaaaagccga
     3961 gccattcgtg cgtcggccgg gcgtagcagt agatgcaccc gtgctcgcag ccgcggtagg
     4021 ggttgatcga cgcctcgatc atgacgtccg ggctgtcgtg acgggtgatg atcgagcgcg
     4081 tggcgtcctt caggtaaacc gtggcgcgtt gcccgccggg ggcaccgccc tcgtgcagtg
     4141 cattgccgtc cggtgcgaag gcgatccgct cgaagcggtt gggcgggtct ccggcggcgc
     4201 cgcgaccttt gatggtgggc tggatgaatt ccatcggacg cgaaaaacgg gtctcgccgc
     4261 gcgggatcgg ccgtgctcag cgggcgacag gaatgttacg ctgtgccgcg tcgcgtggcc
     4321 cgttcggtgg gcgcgctgat gcgagg
//
LOCUS       DATIMH010001205         4731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3462336, whole genome shotgun sequence.
ACCESSION   DATIMH010001205 DATIMH010000000
VERSION     DATIMH010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462336"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..891)
                     /locus_tag="VLI90_20795"
     CDS             complement(307..891)
                     /locus_tag="VLI90_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16716.1"
                     /translation="MSERNAAFDFRQQFLAAESFDPALRQKYQLEVKAMFEHKLTKMH
                     RLVYVFTGLLSIAIIVLFAVISFQLPQRFPPLARAAFGLGILFALGWAIQSYRIVRRG
                     SVKMRKDPRTMAALVWVFIVLMVTINMVLAGRAGDRIWGVQIVVNSLVFLVGAAVFLI
                     GTIVQQAQMQTQEKLLEIEYRLAELTEQIKRPAS"
     gene            complement(888..1463)
                     /locus_tag="VLI90_20800"
     CDS             complement(888..1463)
                     /locus_tag="VLI90_20800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSV16717.1"
                     /translation="MAGSDSRAIYNQLLAIRCRRGDRQAWQELIARFEMPLFCYLRRL
                     VRNEADTWDVLQQTWLGAYRGIGSLHDPSTIAVWLHRIARNLAISHHRRGTGNRGVPE
                     PLPDEALLPDAGDDPPDVQAEMFDDAQRVHRAMDHLSLAHREVLTLHFLQELSLDQMS
                     GVLDVSIGTLKSRLHYARRALRNVLEKEEVV"
     gene            2025..2162
                     /locus_tag="VLI90_20805"
     CDS             2025..2162
                     /locus_tag="VLI90_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16718.1"
                     /translation="MDIVFDRCDDNIVVGVLSSNGSEFGGKESMRLAAQKMTLRREPT
                     Q"
     gene            complement(2295..3731)
                     /gene="gltA"
                     /locus_tag="VLI90_20810"
     CDS             complement(2295..3731)
                     /gene="gltA"
                     /locus_tag="VLI90_20810"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HSV16719.1"
                     /translation="MKAAERMKVERQAMPLLDATDRSATFAEVSLGLAEQIAMREAQR
                     CLGCKEAKCVPGCPVQVDIPAFVSRVADGNFAGAADVLLSCNALPAISGRVCPQERQC
                     EAVCIRSKDKGGSVAIGYLERFVADWARAHREGNNAAASSAASGLRAAIVGSGPAGLT
                     AAGQLARLGHDVTIFEALHEPGGVLVYGIPEFRLPKEIVRAEIDALRQSGVKIVTNVI
                     IGRSYTIDDLLKEEGFHAVFIANGAGLPIFLGLPGEDLKGVYSANEFLTRVNLMRAYD
                     AEADTPVLRPRKAVVIGGGNTAMDAVRTSKRLGANPATLVYRRSRAEMPARLEEVQHA
                     EEEGIIFQMLTNPIEVLADAGGWVRAIRCERMGLGEPDKSGRPRPVPIAGSEFEIECD
                     VIIEAVGTRANPLLTATTPDLKVNEKGYITIDTNGMTSKPGVFAGGDIVRGAATVILA
                     MGDGKRAAAAIDAFLRQKLLTHLDPQPL"
     gene            complement(3728..4594)
                     /locus_tag="VLI90_20815"
     CDS             complement(3728..4594)
                     /locus_tag="VLI90_20815"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HSV16720.1"
                     /translation="MGYRVLQSDWLAPAVRRLVVEAPHVTRHCGPGQFVIVRATANGE
                     RIPLTIAHVDRASKTITLIVQAVGLTTTRLCALKAGEMIADVAGPMGQETEIDCVGHT
                     VVVGGGVGTAVIYPQATALKAKGNTVSAIIGGRSRLNVILQRELCDVCDAVYPCTDDG
                     SHGFKGFVTQRLEQLIVQSAETHSVKLVLCAGPVPMMRAVAELTRPLRIRTIASLNPI
                     MVDGTGMCGGCRISVGGETKFACVDGPEFDAHKVDFKVLIDRLASYREHERHAQERHA
                     DEVTACRGGNQP"
BASE COUNT          848 a         1499 c         1481 g          903 t
ORIGIN      
        1 gacgaacgtg atggatccgg gtattggatc gagccagccg gcgcagacga ctacgactgg
       61 tggattccgc ttgagaaggt cgtcgcggtg aacggcgtgc ccgtcattgg ccggtcgaat
      121 gattagatga cgttccactg cgttgggcgg agcccccggc gtctgcggtt ttttcggtat
      181 tgtcccttca aaaccaacgg cgatcgcgtt cgcgccactt gtagccgcga cgcctgcgtc
      241 gccttcgcct gcaacggcga tgcaggcgtc gcagctacag agcttctcga caatctgccg
      301 ctcgcatcac gacgctggcc gcttgatctg ctcggtcagc tccgccaggc gatattcgat
      361 ctccagcagc ttttcctgcg tctgcatctg cgcctgctgc acgatcgtgc cgatcaggaa
      421 cactgcagcg ccgacgagga agaccaggct gttgacgacg atctgcacgc cccagatccg
      481 gtcgcctgcg cgaccagcca gcaccatgtt gatcgtcacc atcagaacga tgaagaccca
      541 gaccagggct gccatggtcc tggggtcctt gcgcattttc accgagccgc ggcggacgat
      601 gcggtaagac tggattgccc atccaagggc gaagaggatg cccaggccga acgcggccct
      661 ggccagcggc ggaaaacgct ggggcagctg aaacgagatg acggcgaaga gcacgatgat
      721 tgcaatcgaa agcaagccgg tgaagacgta gaccagccgg tgcatcttgg tcagtttgtg
      781 ttcgaacatg gctttaacct ccagttgata tttttgccga agcgccgggt cgaacgattc
      841 ggcggcgagg aactgctggc ggaaatcgaa agccgcgttt cgttcgctca tacgacttcc
      901 tccttttcca ggacattgcg cagcgcccgg cgggcgtaat gcagccggga cttcagcgtg
      961 ccgatggata cgtcgagaac gcctgacatc tgatcgagcg atagctcctg aagaaaatgc
     1021 agcgtcaaca cttcacgatg cgccagcgac aggtgatcca tcgcacgatg cacgcgctgc
     1081 gcgtcatcga acatctcggc ctgcacgtca ggcggatcgt cgccagcgtc tggcaacagc
     1141 gcctcgtccg gcagcggctc gggcacgccg cggtttccag tgccgcggcg gtgatggctg
     1201 atcgccaggt ttcgcgcgat gcgatgtagc cagaccgcaa tcgttgacgg gtcgtgcagc
     1261 gagccaattc cgcgataagc gccgagccag gtttgctgga gcacgtccca tgtgtccgcc
     1321 tcattgcgca ccaaccgccg cagataacag aacagcggca tttcaaaccg cgcgattaac
     1381 tcctgccacg cctgccgatc cccgcgccga cagcggatcg ccagcagctg gttgtagatt
     1441 gctcgtgaat cagatcccgc cattgcgctt gccgtgtgcc ctggtccttt gcctctacag
     1501 tcgcagcaac cgcgggttgg gttcaataga tttcaggatc gtccgtaagg tatcaggcga
     1561 cgaggccgct ccgactttgt cgttgggcgc gcgatgaaga tgttgcgcca aaacgccaag
     1621 aggccaaggc gccaaggatt tcgcgacaac gtctagcacg gacgtctccc ccgtgccaaa
     1681 gcgtccaggc gcgggcgaga tgccagtgct acgaatgaga aatctctttt tgccttggcg
     1741 tctcagcgtc tcagcgtctc agcgtctcag cgtcttggcg gccttggcgc taatccgaat
     1801 ctaaacgggt tatgagagac gaaacttgct ttgcgtaaag ccggcgctgg cttcgccgcg
     1861 accggtgcgg ggaccgacgg tgcgatcgcc ggctgaacag taatggccac ctggtcgccg
     1921 gtcgcggggc gatcgagcgc tcgctgggct ggggacgcga ttaccgcatt cacgccaggc
     1981 tggccggcga catcatcaag gccgaaggca tagggcgtga gtcggtggac atcgtcttcg
     2041 atcgatgcga tgacaacata gtggtcggcg tgctgagttc caacggctcg gagttcggcg
     2101 ggaaagagtc gatgcggctg gcggcgcaga agatgacgct gaggcgtgag ccgacgcagt
     2161 gatcgagccg cgagaatgtc cgtcacgcaa acggcgttgg gtcgctcgta ttcgaccatg
     2221 cctcttgtag gggcgaggcc tgcctcgccc gatttcagcg gacgcagtta aagagggcga
     2281 cgcaggcgtc gccgctacaa gggttgtggg tcaagatggg tcaggagttt ctggcgcaaa
     2341 aacgcatcga tcgccgcggc ggcgcgtttg ccgtcgccca tcgcgaggat gacggtggcg
     2401 gcgccgcgta cgatgtcgcc gccggcgaag acgccgggtt tgctcgtcat gccgttggtg
     2461 tcgatcgtga tgtagccttt ttcgtttacc ttcaggtccg gcgtcgtcgc cgtgagcagc
     2521 gggttggccc gcgtgccgac ggcttcgatg atcacatcgc attcgatttc aaattcgctg
     2581 ccggcgatcg ggaccggtcg cgggcggccg gatttgtcgg gttcgcccag gcccatgcgc
     2641 tcgcagcgga tcgcacggac ccagccgccc gcgtcggcaa ggacctcgat cgggttcgtc
     2701 agcatctgga agatgattcc ctcctcttcc gcgtgctgaa cttcttccag tcgagccggc
     2761 atttccgcgc gcgagcggcg atagaccagt gtcgctggat ttgcgccgag ccgcttgctc
     2821 gtgcgcacgg cgtccatcgc ggtgttcccg ccgccgatga ccaccgcctt tcgcgggcgc
     2881 agcaccggcg tgtcggcctc ggcgtcgtag gctcgcatca ggttcactcg cgtgagaaat
     2941 tcgttggcgc tgtagacgcc tttgagatct tcaccgggta gtcccaggaa aatcggaagg
     3001 cccgcgccgt tggcgatgaa gacggcgtga aagccttctt ccttcagcag atcgtcaatg
     3061 gtgtacgagc ggccgatgat cacgttcgtc acgatcttga cgccggactg gcgcaacgcg
     3121 tcgatctcgg cccgcacgat ttccttgggc agccggaatt cgggaatgcc gtagacgagc
     3181 acaccgccgg gttcgtgcag ggcttcgaag atcgtcacat catgaccgag ccgggcgagc
     3241 tgtcctgcgg cggtgagacc ggcggggccg ctaccgacga tggcggcgcg gaggccgctt
     3301 gccgccgatg acgcagcggc attgttgccc tctcgatgcg cacgagccca atccgcaacg
     3361 aagcgctcca gatatccgat ggcgacggag ccacctttgt ccttggatcg aatgcagacc
     3421 gcttcgcact gccgctcctg cgggcagacc cggccggaga tcgcgggcag cgcgttgcaa
     3481 ctcagcagca catcggccgc gccggcgaaa ttgccgtcgg ccacgcgact cacgaacgct
     3541 gggatatcca cttgcaccgg gcaacccggc acgcacttgg cttccttgca gccgaggcat
     3601 cgctgcgctt cgcgcatcgc gatctgttcg gccagaccga ggctgacttc ggcgaacgtc
     3661 gccgatcggt ccgtcgcatc cagcagcggc atcgcctgcc gttcgacctt catgcgttcg
     3721 gcagctttca cggctggttc cctccgcggc aggcggttac ctcatcggca tgccgctcct
     3781 gcgcgtgccg ttcgtgctcg cgatacgacg ccagccgatc gatcagcact ttaaaatcca
     3841 ctttgtgtgc gtcgaactcc ggcccatcga cgcaggcgaa tttcgtctcg ccacccacgc
     3901 tgatccggca cccgccgcac atgccggtgc catcgaccat gatcgggttg agcgatgcga
     3961 tcgttctgat gcgcagcggc cgggtcagtt cggcgaccgc gcgcatcatc ggcacggggc
     4021 cggcgcacag gacgagcttg accgaatgag tctccgcgct ctgcacgatc agctgttcca
     4081 gccgctgtgt aacaaaacct ttgaagccat ggctgccgtc gtcggtgcag ggatagacag
     4141 cgtcgcacac atcgcaaagc tcgcgttgta ggatgacgtt caaccgcgat cgcccgccga
     4201 tgatggcgga tacagtattt cccttcgcct tcagcgccgt cgcctgcgga taaatgaccg
     4261 cagtacccac gccgccaccc acgacgacgg tgtggccgac gcagtcgatc tccgtctcct
     4321 gccccatcgg cccggccacg tcggcaatca tctcgccggc tttgagcgcg cacagtcggg
     4381 tcgtagttag gcccaccgcc tgcacgatca gcgtgatcgt tttcgatgca cgatcaacat
     4441 gcgcgatcgt cagcgggatt cgctcgccat ttgcggtcgc gcgcacgatc acgaactgcc
     4501 ccggaccgca atggcgcgtg acgtgcggcg cttcaaccac cagccggcgc accgccggcg
     4561 cgagccagtc ggattggagg acgcgatatc ccattcgatt cactgcattc ggcgcacgcg
     4621 cagcgggacg aagtcgcaat tcggttcttc cgccaggtaa ttcttcgtat gtccatacgc
     4681 gcgagcgcgg cagcccatgc agaccttgct gtattcgcac acgccgcact t
//
LOCUS       DATIMH010001206         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_284052, whole genome shotgun sequence.
ACCESSION   DATIMH010001206 DATIMH010000000
VERSION     DATIMH010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284052"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..315)
                     /locus_tag="VLI90_20820"
     CDS             complement(145..315)
                     /locus_tag="VLI90_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16721.1"
                     /translation="MDSSEESGEGRSNEKGCVVGGAAASAARAAVRREIDKPIQPLQH
                     LTLRRIRVVLKA"
     gene            335..1162
                     /locus_tag="VLI90_20825"
     CDS             335..1162
                     /locus_tag="VLI90_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16722.1"
                     /translation="MMMAPPESESVYAPPEPPREEEGVNNGGVNFDLTVRYVTDYVYR
                     GISHNRAAGGNEHASNFQADTKLSFNLGRLPHPYVGVFANVNDSDPLSRFQEIRPVFG
                     MEYTLRPLILNIGHNTYIYPERERLNPSPNTSEVFAQITVDDSYFFLTPQPILSPYIY
                     AAYDYQRNEGWYLEAGVKHDFIFEDAGFTLTTYGDVGYLSEFPQQFVLVSPKDSGFQH
                     YDLGIIASLSLNHLFNLATPRFGQFAIEGYLTYTGRFSNPLLADSILWGGVGLAFKY"
     gene            complement(1351..1746)
                     /gene="atpC"
                     /locus_tag="VLI90_20830"
     CDS             complement(1351..1746)
                     /gene="atpC"
                     /locus_tag="VLI90_20830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HSV16723.1"
                     /translation="MAFQCTIVTPEAQALDESIQQAILPAHDGLIGILTDRAPLLVKL
                     GVGPLRVDLAGGQQRFFLIDGGVAQMKDNKLTVLTTVATPASEIDAATARAEYAEAVA
                     RRPIDAPTAEARQHQMERARAAEAIAGKR"
     gene            2071..2364
                     /locus_tag="VLI90_20835"
     CDS             2071..2364
                     /locus_tag="VLI90_20835"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16724.1"
                     /translation="MQMMSAAEKAALEQKREEMYKAKIDVQERIRKAASLGDLSENAE
                     YHFAKEENRSLERELKALEDKLKNVAVVATDNVPDDMVFLGHTVKLLDLYDDS"
BASE COUNT          465 a          768 c          712 g          456 t
ORIGIN      
        1 gaaccaaagg cgcccagccc gtcgcatcgc gtcccatcgc agccgcgggc taaagccccc
       61 gcggctgcga tttgtgtgcg atccgctgta accgaatcaa tgcgccgttc gtgacaacct
      121 ccgcccacct cggtatcgtt cgcttcaggc tttgaggacc acgcgaatgc gacggagcgt
      181 caggtgctgc agcggttgga tcggcttgtc gatctctctc ctcaccgccg cacgcgctgc
      241 gctggcggcg gcaccgccga ccacgcaacc cttttcattc gacctgcctt caccggactc
      301 ttcacttgaa tccatctcat catcgccgcc gcggatgatg atggcgccgc cggaatcgga
      361 aagcgtttac gcgccacccg aaccgccgcg ggaagaggaa ggcgtcaaca acggcggggt
      421 gaacttcgat ctcaccgtgc gctatgtcac ggattacgtc tatcgcggga tcagtcacaa
      481 tcgcgccgcc ggcggcaacg agcacgcgtc gaactttcag gcggacacca agctgtcgtt
      541 caacctcggc cggctgccgc atccgtacgt cggggtgttc gcgaacgtca atgattccga
      601 cccgctgagc cggttccagg aaatccgtcc ggtcttcggc atggagtaca cgctgcggcc
      661 gctgatcctg aacatcggcc acaacaccta tatctacccg gagcgcgagc ggttgaaccc
      721 gagcccgaac acctcggaag tgttcgcgca gatcacggtg gacgattcgt atttcttcct
      781 cacgccgcaa ccgatcctct cgccgtacat ctatgctgcg tacgactatc agcgcaacga
      841 aggctggtat ctcgaagcgg gcgtcaaaca cgacttcatc ttcgaagacg ccggcttcac
      901 gctgacgacg tacggcgacg tcggatatct ttcggagttt ccgcagcagt tcgtgctcgt
      961 ctcgccgaaa gacagcggat tccagcacta cgacctgggc atcatcgcat cgctgtcgct
     1021 gaaccacctg ttcaacctgg ctacgccaag gttcgggcag ttcgcgatcg agggctacct
     1081 gacgtacacg ggaagatttt caaacccgct gctggcggat tcgattcttt ggggtggggt
     1141 ggggttggcg tttaagtatt aaatgatccc ttgtcctgag cgcaggcgta ctcgccgaag
     1201 tcgaaggata tagctggaag taggggaaca ctggcttgcg cgaacggcgc gagagtcgct
     1261 tgcggatttg cgcgcggaaa ggttcacata cttctggcaa tacccttcga cttcgcggct
     1321 gcgccgctgc gcttaagata aagacagcac ctaccgtttc cccgcgatcg cttccgccgc
     1381 tcgggcccgt tccatctgat gctgccgcgc ctccgcggtg ggcgcatcga tcggccttcg
     1441 cgccaccgct tcagcgtact ccgcccgcgc ggtcgcggcg tcgatctcgc tggccggcgt
     1501 cgccaccgtc gtcagcaccg tcagcttgtt gtccttcatc tgcgccacgc cgccatcgat
     1561 caggaaaaag cgctgctgtc ccccggccag gtccacgcga agcggtccaa cgccgagctt
     1621 gaccagcagc ggcgcgcgat cggtgaggat gccaatcaac ccgtcgtgag cagggaggat
     1681 ggcctgctgg atcgactcat ccaaagcctg agcctcgggg gtgacaatcg tgcattgaaa
     1741 tgccatggtc gcgcgctccg ctgtgatgca gggtgatgcc ggaatcgccg gctctgcccg
     1801 gcgagaacgc ggagtttaga gccgcccagc ggcgccgtca aaccctgcca aacatgccga
     1861 aaattattgc gttagcccgt ttccgaattt cacaagccgg gatatcatca tccgctgttg
     1921 aacgcccgct gcccaccgcg ccgaccgcgc ggtggctcgc attttgggcg ggaccatttg
     1981 tcgaaaagat cgcgtcgaac gccgctcgtg taagcccatt tgtgtttagc gccggcacga
     2041 cgcgccttaa tgcgttcagg agagtttcgc atgcagatga tgagcgctgc ggagaaagcc
     2101 gccctcgagc agaagcgcga ggaaatgtac aaggccaaga tcgacgtgca ggagcgcatc
     2161 cgcaaggccg ccagcctcgg cgacctgtcg gagaacgcgg agtatcactt cgccaaggaa
     2221 gagaaccgca gcctggagcg cgagcttaag gcgctggaag acaagctgaa aaacgtcgcc
     2281 gtcgtcgcca ccgacaacgt gccggatgac atggtcttcc tcgggcacac cgtgaagctg
     2341 ctggacctgt acgatgacag ctagcagttg gtccgcctgg ttggtgaagc atcggcgccc
     2401 g
//
LOCUS       DATIMH010001207         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3753742, whole genome shotgun sequence.
ACCESSION   DATIMH010001207 DATIMH010000000
VERSION     DATIMH010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3753742"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1618)
                     /locus_tag="VLI90_20840"
     CDS             complement(<1..1618)
                     /locus_tag="VLI90_20840"
                     /inference="COORDINATES: protein motif:HMM:NF025965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HSV16725.1"
                     /translation="MTYRVILRVMLAATVGVATSAAAQSSQPATITATHGVYPNLVGN
                     TDRPLRYRPNGADFVIENGQEFFNRPLYGRNTAARVDAGDKPEFTIYLPGRGGNLRLG
                     VRSPARKSKWLFDAARVITRYRPGMMLYEIHDPLLGANGVLNVSVVALDATDGLAVRA
                     EAKSIDSGVELVWAYGGVNGKRGTRDGDIGTERVPISEYFQLKPEFCQNNQFTIDGRR
                     FTLRSKPAMIVGLMPRGSMVGVADANQWASFDALLASVAPTPPARPVVIGHVVLPANG
                     PLFLALQRVSESTVANDDLQTYRDLTTTRPDEPSASPAKVSFIPAYDPDDIPRVFAEA
                     ERHYAALRERIAVDTPDPYINAAVGALNLASDAVWDDPQQNIMHGAIAWRTRLLGWRG
                     PSMLDALGWHDRARKYLTYWAAQQNTDPIPAKLPGPDEKANLSRSERALHSNGDLSRS
                     HYDMNLVYIDTTFRHLLWTGDVELARQLWPVIERHLAWERRLFRREFGPEKLPLYEAY
                     ADIWASDDIQYSGGGVSYSSAYNCYHNRMAA"
     gene            1707..2552
                     /locus_tag="VLI90_20845"
     CDS             1707..2552
                     /locus_tag="VLI90_20845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16726.1"
                     /translation="MNHRWLALVIVTSLLIATAPTASAAAGPATSPNDLITRTYAIRD
                     LLWVMSAPNYSNVRFPAAATAAASPATQPDFAANAARFTRMLRDALPAQIDGSAIVMT
                     NDGVNLTVTTPAATQVVVADAFEKLRSDMLVQVSDEASIYSLPPATFQRLSFSIPKIN
                     RFDDATGILAMPLSEEQVDELQMKVMGDRQASRLSPPRLTLFNHQKALILVQTEQAYV
                     SGYTFATRWPDDVQACRRDVPAGWLLVVGSSDRSSRSPIEHASGAPGRVPPGPAGSRH
                     IRWPG"
     gene            2566..2706
                     /locus_tag="VLI90_20850"
     CDS             2566..2706
                     /locus_tag="VLI90_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16727.1"
                     /translation="MPVQSALSVEKTIALPDKQAMLVAAQTDAITGEHQLIVVRSTIL
                     WH"
BASE COUNT          458 a          886 c          864 g          511 t
ORIGIN      
        1 gcgcggccat gcggttgtga tagcagttgt acgccgatga atagctcacg ccgccgccgc
       61 tgtattggat gtcgtcgctg gcccagatgt cagcgtacgc ctcgtatagc ggcagttttt
      121 cgggtccgaa ctcgcggcgg aacagccggc gttcccatgc caggtgccgc tcgatgaccg
      181 gccacagttg ccgggcgagc tcaacatcgc ccgtccacag caggtgacgg aaggtcgtgt
      241 cgatgtacac gaggttcatg tcgtaatgcg atcgcgacag gtcgccgttg ctgtgcaacg
      301 cgcgctcgct gcgcgacagg ttcgccttct catccggccc cggcagcttc gcgggaatcg
      361 gatcggtgtt ctgctgggcc gcccaatagg tgaggtactt tcgcgcccga tcgtgccacc
      421 ccagcgcatc gagcatgctc ggcccgcgcc agccgagcag ccgcgtgcgc caagcgatcg
      481 ccccgtgcat gatgttctgc tgcgggtcgt cccacaccgc gtcgctggca aggttcagcg
      541 cgcccacggc ggcgttgatg tatggatccg gcgtgtctac cgcgatgcgc tcgcgcaatg
      601 ccgcgtaatg ccgctcagcc tcggcgaaga cgcgagggat atcatcggga tcgtacgcgg
      661 ggatgaagct aaccttcgcg ggcgatgcgc tcggttcatc cggccgcgtc gtggtgaggt
      721 cgcggtacgt ctgcagatca tcgttcgcca ccgtcgattc agaaacgcgc tgcagcgcga
      781 ggaacagtgg cccgttcgcc ggcaacacga cgtggccgat caccaccggc cgcgccggtg
      841 gcgtaggggc gaccgatgcg agcagcgcat cgaacgacgc ccattgattg gcgtccgcca
      901 caccgaccat cgaacctcgt ggcatcaggc cgacgatcat cgcgggcttg ctgcgcagcg
      961 tgaaccgccg gccgtcgatc gtgaactgat tgttctggca gaactccggc ttgagctgga
     1021 aatattcgct gatcggcacg cgctcggtgc caatgtcgcc gtcgcgcgtg ccgcgcttgc
     1081 cgttgacgcc gccgtaggcc cagaccaatt ctacgccgct gtcgatcgat ttcgcttcgg
     1141 cgcgaacggc caagccatcg gtcgcatcga gggcgaccac gctgacgttc aggacgccgt
     1201 tcgcgcccag cagcggatcg tggatctcgt acagcatcat ccccgggcga tagcgcgtga
     1261 tgacgcgcgc cgcatcgaac agccacttcg attttcgcgc gggcgaacgc acgccaagcc
     1321 gcaggttccc accgcggccc ggcaggtaga tcgtgaattc cggcttgtcc cccgcatcca
     1381 cgcgagcggc ggtgttgcgg ccgtagagcg ggcgattaaa gaactcctgc ccgttctcga
     1441 tgacgaagtc ggcgccattc ggccgatacc gcagcggccg gtcggtgttg cccacgagat
     1501 tcggatagac gccgtgcgtc gcggtgatcg tggctggttg cgacgattgc gcggcggccg
     1561 acgttgcgac gccaacggtc gccgcgagca tgacgcgaag gattacgcgg taggtcattt
     1621 tcgatttggc tccaggcgag cgatcatatc ggccggccgc ggattttgct ttcgccgttc
     1681 gcgccacgca gatagcattc cgcgtcatga accaccgctg gctcgctttg gtgatcgtga
     1741 catcgctgct catcgcgact gcgcccacgg cttccgccgc ggcaggaccg gcgacttcgc
     1801 ccaatgatct catcacgcgg acctacgcca tccgcgatct gctgtgggtc atgtccgccc
     1861 ccaactattc caatgtacgc ttccccgccg ccgctaccgc cgcggcatca ccggcgacgc
     1921 aaccggactt cgccgccaac gcggcgcgct tcacgcggat gcttcgcgat gcgctgccgg
     1981 cgcagatcga tggcagcgcg atcgtcatga ccaacgacgg cgtcaatctc accgtcacca
     2041 ctccggcggc cacccaggtg gttgtcgccg atgcgttcga gaagctgcgc tcggacatgc
     2101 tggttcaagt gtccgatgag gcgagcatct acagccttcc accggcgacg tttcagcggc
     2161 tgtcgttttc gattccgaag atcaaccgat ttgacgacgc gacgggtata ctggcgatgc
     2221 cgctgagcga ggagcaagtc gatgaacttc agatgaaggt gatgggagat cggcaggcct
     2281 cacgattgtc gccgccgcgt ctcacgctgt tcaatcacca gaaggccctc atcttggtgc
     2341 agacggaaca ggcatacgtc agcggttata cgtttgcgac ccgatggcca gacgacgttc
     2401 aagcctgccg tcgcgacgtt cccgccggtt ggcttctcgt ggtcggttca agcgaccgtt
     2461 ccagccgatc accgatcgag cacgcttcag gtgcacctgg acgcgtcccg cctggtccgg
     2521 ctgggtcgcg gcacattcgg tggccgggat gatctaatgg ttcaagtgcc ggtacagtcg
     2581 gcgctgagcg tggagaagac gatcgccctg ccggacaagc aggcgatgct ggtggccgcg
     2641 cagaccgatg cgattactgg ggagcaccag ctcatcgtgg tgcgctcgac gatcctttgg
     2701 cattagcctt gatacccct
//
LOCUS       DATIMH010001208         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_3753821, whole genome shotgun sequence.
ACCESSION   DATIMH010001208 DATIMH010000000
VERSION     DATIMH010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3753821"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VLI90_20855"
     CDS             complement(<1..1151)
                     /locus_tag="VLI90_20855"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HSV16728.1"
                     /translation="MAEEIKPIDPQPARRLSRRAMAGFIVLALVTAVIIALATAMLVN
                     IWTRKQEARSTFVRLVDVNENTTDPVPWGTNFPREYDQYLRTVDNTRTRYGGSDAMPA
                     QKLDRDPWLRRMFAGYAFSLDFRDRRGHAYMLYDQTHTERVLKRPQPGACLHCHASII
                     PTYRRVGAAALGVEPANPNEFDWTAVMKGFELVTAMKYAVAFAEVQKTPDGALLNEKQ
                     TPNGSNDLPETTPRGDPTAPATTQQSLAVHKVGNAHPVSCVDCHDPQTMELRVTRPGF
                     IRGIQALANSYDPVTHLPSIGRWRTGNRLQPYDPNRDAGRQEMRSFVCGQCHVEYYCG
                     PKVTLFFPWNNGLKVEQIESFYDSYKFPDGHRFYDFTHAETGAEIIKAQ"
     gene            complement(1189..1650)
                     /gene="nrfH"
                     /locus_tag="VLI90_20860"
     CDS             complement(1189..1650)
                     /gene="nrfH"
                     /locus_tag="VLI90_20860"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HSV16729.1"
                     /translation="MRLRHFAIIFAVMVGVAVGVSTFTFHYARGASYLSNDPKACVNC
                     HIMNDQYDGWLQSPHHAVAVCNDCHVPHDFVGKWIAKAANGYHHSKAFTLQNFHEPIM
                     ITPHNADTLQANCLRCHGDLVSEIVHGSRETHVGDGTIKCVQCHRRVGHGP"
     gene            complement(1898..2626)
                     /locus_tag="VLI90_20865"
     CDS             complement(1898..2626)
                     /locus_tag="VLI90_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16730.1"
                     /translation="MRFIRIILSLGATAMAIAGCAPANKCQPLEPGLAALEIKAVAEP
                     KNGASPIDSDAYGTSSPTGRFQLLDYDNIQGLVVWVEPTDHVAAPPTTVGEITIEFDE
                     HGQGGGGVVQGVAVGTRLIVVNRSEKPQSLYCVSDGNEAASRTVAPGRQTEFSMRTPG
                     LVEIVSESMDEPAARVYAVASRWVRTLHAGESTCFNDLPPGGYRAACWHERLPGSQQP
                     ITLAAGTHSQLTLTASVNVLPVHP"
BASE COUNT          468 a          807 c          847 g          538 t
ORIGIN      
        1 tgctgcgcct tgatgatctc cgcgccagtc tcggcgtgcg tgaagtcgta aaagcggtga
       61 ccgtcgggga acttgtagct gtcgtaaaac gattcgatct gctcgacctt caggccgttg
      121 ttccatggaa aaaagagcgt gaccttcggt ccacagtaat actcgacgtg gcactgtccg
      181 cagacgaacg accgcatttc ctgccgcccg gcatcgcgat tggggtcgta cggctgcagg
      241 cggttgcccg tgcgccaacg accgatgctc ggcaggtgcg tcaccggatc atacgaattg
      301 gccagcgcct gaatgcctcg aatgaacccc gggcgggtga cgcgcagctc catggtttgc
      361 gggtcgtggc aatcgacgca tgataccgga tgtgcgttgc ccaccttatg aacggccagc
      421 gactgctgcg tggtcgccgg tgccgtcgga tcgccccgcg gggtggtctc gggtagatca
      481 ttcgatccgt tcggtgtctg tttctcgttg agcagcgcgc catccggcgt cttttgaact
      541 tcggcaaagg cgacggcgta tttcatcgcc gtgacgagct cgaatccctt catcaccgcg
      601 gtccaatcga actcgttcgg attcgccggc tcgacaccca gcgccgcagc accaacgcga
      661 cgataggtgg gaatgatcga agcgtgacaa tgcaggcacg cgccgggttg cggccgcttc
      721 agcacgcgct cggtgtgcgt ctggtcgtac aacatgtacg catgtccccg gcgatcgcgg
      781 aagtccaagc tgaacgcgta gcccgcgaac atccgccgca accacgggtc gcggtccagc
      841 ttctgcgccg gcatggcgtc gctgccgccg tagcgcgtgc gggtgttgtc caccgtgcga
      901 aggtactggt cgtactcgcg cgggaagttg gtgccccacg gcaccgggtc cgttgtgttc
      961 tcgttgacgt ccaccagccg cacgaacgtg ctgcgggcct cctgcttgcg cgtccagata
     1021 ttgacgagca ttgccgtcgc cagcgcgatg atcactgccg tcaccagcgc cagcacgatg
     1081 aaccccgcca tcgcgcgccg gctcaatcga cgcgccggtt gtggatcgat cggtttgatt
     1141 tcctcagcca cgattcacct caattccctt gatttggcct gcgtccgatc acggcccgtg
     1201 tcccacccgg cggtggcatt gcacgcactt gatcgtgcca tcgccgacgt gcgtttccct
     1261 gctgccgtga acgatctcgg acacgagatc gccgtgacat cgcaggcagt tggcctgcag
     1321 agtgtccgcg ttgtgcggtg tgatcatgat cggctcgtga aagttctgaa gtgtgaaggc
     1381 cttcgaatgg tgatatccgt tggcggcctt ggcgatccat ttgccgacga agtcgtgcgg
     1441 gacatggcag tcattgcaca cggcaaccgc gtgatgcgga ctttgcaacc acccgtcgta
     1501 ctggtcgttc atgatgtggc aattcacgca ggccttggga tcgttgctca gatatgaggc
     1561 gccgcgggca taatgaaagg tgaacgtgct gacgccaacg gcaacgccaa ccatcacggc
     1621 gaagatgatc gcgaaatgcc gcagccgcat agggtgctat atcttcgtta tcccgacatc
     1681 gcgggatgat aacgccatct gccgagtttg ctgacatctt tttgacaatg ggctgtgtcg
     1741 aagatgacaa cggacgttac aaccgcggcg atgcgggaac atcggggcag atgcttgaga
     1801 tgatgtcagg agaaacgcct agactcgcgc gagccgcagt cacggcccca tgtgcgggta
     1861 aacgtgcggt tcagttcaag tgccgcactg cccgccacta tggatgcacc ggcagcacgt
     1921 tgacgctcgc agtcaacgtt aactgcgagt gcgtgccggc ggcgagcgtg atgggttgct
     1981 gtgaaccggg caggcgctca tgccagcagg cggcgcgata gccgccgggc ggaagatcgt
     2041 tgaagcatgt ggattcgccg gcatggagcg tgcggaccca gcgactggcg acggcgtaca
     2101 cgcgggccgc gggttcgtcc atcgattcgg agacgatctc gacgaggcct ggcgtgcgca
     2161 tggagaattc cgtctgcctg cccggcgcga cggtgcgcga cgcggcttcg ttgccgtcgg
     2221 agacgcaata tagtgactgc ggcttttcgc tgcgattgac aacgatcagt cgcgtgccga
     2281 cggcgacgcc ttgcaccacc ccaccgccgc cttggccatg ctcgtcgaac tcgatcgtaa
     2341 tctcgccgac agtcgtcggc ggcgcggcta cgtgatccgt gggttcaacc cacacgacca
     2401 agccctggat gttgtcgtaa tcgagcagtt ggaatcggcc ggtgggtgag gacgtgccgt
     2461 aggcatcgct gtcgatcggc gatgcgccat tcttgggttc ggcgacggct ttgatctcca
     2521 gcgccgccag ccccggttcc agcggttggc acttgttggc cggcgcgcag ccggcgatcg
     2581 ccatcgccgt ggcgccaagg ctcagtatga ttcggatgaa cctcatttac ggtgaagctc
     2641 cacgttgatc gtctgcggcc
//
LOCUS       DATIMH010001209        10023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4038728, whole genome shotgun sequence.
ACCESSION   DATIMH010001209 DATIMH010000000
VERSION     DATIMH010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4038728"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="VLI90_20870"
     CDS             complement(<1..1623)
                     /locus_tag="VLI90_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16731.1"
                     /translation="MTTDAEQKRLDEDRLSHVNWRRWGPYLSERQWGTVREDYSADGS
                     AWTHFSHDQARSRAYRWGEDGIAGISDNEQRLCFAVAMWNGKDSILKERLFGLTGPEG
                     NHGEDVKELYYYLDATPTHSYLKMLYKYPQAEYPYTRLVEENRYRGKSAPEFELIDTG
                     VFNDGRYFDVFVEYAKAGENDILIQITVHNRGPKDAPLHLLPQMWFRNSWSWNAGSPK
                     PVMRAGGDEGPIEIEHVELGQYALFADGGPQLLFTENETNGPKLFGLPHVDGAYYKDA
                     FHEFVLGGNVAAVNPNRSGTKAAALCVLSVPAQGSQRVRLRLMRTAAPASSGPGAEWG
                     MGDLGMPLKTPDQNHPHPNPLPEYRARGPEGAFAEFDSTFERRRREADAFYSDIQTGI
                     DDADARLVQRQAWAGMIWSKQFFYYNIGEWLNGDPAGPPPPASRKNGRNAEWMHLNNA
                     NIISMPDKWEYPWYAAWDLAFHCIPFAMIDPEFAKRQLVLLTREWYMHPNGQLPAYEW
                     AFGDVNPPVHAWAALRVYQIDKDRRGGVGDVAF"
     gene            2031..3491
                     /gene="cls"
                     /locus_tag="VLI90_20875"
     CDS             2031..3491
                     /gene="cls"
                     /locus_tag="VLI90_20875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HSV16732.1"
                     /translation="MPNAHWLPPEWFVVYITIAWCIRAAMVPVVLRREFAPGAALAWL
                     GIVFLHPYIGLTLYMIVGETRLGPRRAEAHRALVRRSRPLRRTDDAHTHRVELPALQH
                     SYDTMVLQAEKISGMPIVGGNQVDFIGESTTLIDGLVADIDAATTEVHLLYFIFACDK
                     TGGRVAAAVEAAARRGVKCRLLVDAVASRYLLHHRGLSKRLREAGVVLAAALPVAPIS
                     RQLPRMDLRNHRKLAVIDGRVAFTGSHNLINPDYGRTRCGPWVDLSARFSGPVVAELA
                     AVFNEDWAFETGEELPLPTIDQSVPMDDGVPAQVVPTGPSPPGDTFRRVLLAAVQSAR
                     KRLTLTTPYFVPDEPTLVALQMAADRGVEVTLIVPQHPDHPFTAAAGRAQYGRLMQAG
                     VAIWQYQPGLIHAKTTTVDHAFAVLGSANLDVRSFNLNFELSVLLYGPDVTERLRAIQ
                     MKYLADSHRIDPETWSRRPVVKQYAERAVALLSPLL"
     gene            complement(3564..5219)
                     /locus_tag="VLI90_20880"
     CDS             complement(3564..5219)
                     /locus_tag="VLI90_20880"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSV16733.1"
                     /translation="MQHFALAAAAALSLSLFSSIQTARADETTAPSEPMAAGPFQPSW
                     DSLKQYKTPVWFSDAKFGIWAHWTAQCVPEQGDWYARNMYIEGQHDYKYQVEHYGHPS
                     KVGFKDIDNLWHAEHWEPEKLIALYKRAGAQYFVALANHHDNFDCFDSKYQPWNSVNI
                     GPKKDIVGTWEKAARAAGLRFGVTVHAAHAWSWFEVAQGADKTGPLAGVPYDGKLTKA
                     DGKGTWWEGYDPQDLYAQNHTPGKKLVWDWQADQGSSVPDKAYCEKFYNRTVDLINKY
                     HPDLLYFDDTVLPLNNVSDVGLRIAAHYYNSNSAQHDGRNEAVMNTKGLNEEQRQCLV
                     WDIERGLADKIEPFAWQTDTCIGSWHYDRSIYDRHRYKTAKTVIQMLADIVSKNGNLL
                     LNIPVRGDGTIDDDELKVVEGIASWMDVNKEAIFGTRPWVTFGEGPSAEPKTPSTQPG
                     KKVPNFNEGKAGFTAKDIRFTTKGDTLYAIAFGWPSDGKLTIKSLAKGSDKYTHDVGA
                     VHLLGADAALQADRSDDGLNITLPAEKPAGDTFVFKIDPAAEK"
     gene            complement(5225..6340)
                     /gene="proB"
                     /locus_tag="VLI90_20885"
     CDS             complement(5225..6340)
                     /gene="proB"
                     /locus_tag="VLI90_20885"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HSV16734.1"
                     /translation="MESTRTQYLSNARLLVVKLGTQLLTDKAGRLDAPFLATIAKQVA
                     SLRVRGVLVTIVSSGAIGAGVGELKLPKRPTDLAKLQAVAAIGQRKLMDAWADAFEPH
                     AIHVAQVLLTREDIDDRTRFLNVRNTIHAVHELGGVPIINENDAISTDELVKITFGDN
                     DILAALVAHALRADLLVLLSVVDGVLDTSGKSVRLVESVDAARELVRTEKSALGKGGM
                     DSKVNAARMVTDAGSAMVVADGRMENVLARIVAGDEVGTLFVPGTRKMSSRSRWIGAA
                     RPSGTIVVDAGAVTALVEKNRSLLPAGIVKVDGTFARGDIVAIADGDGQVIARGLSNY
                     AAADIERIKGKRTADVRAALAESAYDEVVHRDNLVIG"
     gene            complement(6462..8480)
                     /locus_tag="VLI90_20890"
     CDS             complement(6462..8480)
                     /locus_tag="VLI90_20890"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSV16735.1"
                     /translation="MNTRAHTADVSVSFEVLESRQLFSVGLNTAGWTQVTPSSDTRTI
                     YVSNSTGSDANTGLSASSPVKSLSKAASLLRDGSPDWMLLKRGDTWSGSSFPTWNKSG
                     RSSSEPMVIGTYGSGNRPTIDTGASGGFSATTGHAVNNLVLTGVNLYADQRDPNSRTF
                     NSATIGSRPTGLLFTGPGSNVTIEDCSFRFFKDNVVLEGVSNMTVRRCVIADAYCTAD
                     RSQGFYSMNASGITLDQNVFDHNGWNQQISGAGDTGYNHNIYYSASALNMTVRGNIIA
                     DASSTGVLARGGGVIENNLFLHNAVGISYGNANGADSTPGGVTGRITGNVFIDDKGIA
                     AGKMGIGIEVGNIKPNSGFTISSNIFTHDTQQNAAAISLTYASITNNPGSCVGINDVT
                     IANNITGGWRNALTISGGFVDGGSGLKALNDLTITGNDFEQSLSVVTRHSNAFNSSAE
                     HWSGNNYCTSSMTSGNQFAIGSTNMSISGWQKAIESSATNVVKSYPDRNRTAATYNAT
                     LGGAATLDAFLAQADQQSTDNWQAAYTADAVITYVRQGFGLGSGGAPAPAPAPAPAPM
                     GSISGTVFNDVNANLKRDSGEGALSGWKVYIDANNNHQFDAAEKYVTTDASGNYKLTN
                     VAAGYHIVRVIRPTGWTQTLPTGDMGQHITLKAGQNLTGALFGERRIS"
     gene            complement(8765..>10023)
                     /locus_tag="VLI90_20895"
     CDS             complement(8765..>10023)
                     /locus_tag="VLI90_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16736.1"
                     /translation="RAARRHAGDHAHLATFLAAITLLNFGWATYTGFQLETIQCFFTC
                     LAAAAALEVIGGGIATTGSLLADAFVAGLAAGSAAMVKPTALAVGGGLGLALLLQVHS
                     RGWRTVMACMAAVIAGVAVPTLAVAYWVWKTGIAGEMPNLFRQIAWYGSKSPMDSFTV
                     TKLFIAGVMCAQPLVVRGWVFRRDVVPVERRIGPSQAMFAFVIAWFALELAGIIMQGR
                     MYLYHFLPLAPPLALLYGVLPRTARAPVIGLGLIPIALMSMHWPGSKLVRANEGTQHK
                     AISQYLIDHTKPGDAVFADQAGRILLETGLRPGSRYGIFYYWVNYDNAPQDYCSRMLA
                     DFDERKPKYIVLAYDADDAMAEMLTGPILGLRPVRSRNTYLAWRQFKQYLSAHYQFET
                     RVDDNDLYRIRQEPSATARIESGDAP"
BASE COUNT         1715 a         3291 c         3082 g         1935 t
ORIGIN      
        1 gaacgcgaca tcacccacgc cgccccggcg gtccttatca atttgatata cccgcagcgc
       61 cgcccacgcg tgcaccggcg ggttgacgtc accgaacgcc cactcgtacg ccggcagctg
      121 cccgttgggg tgcatgtacc attcgcgcgt gagcagcacg agttgccgct tggcgaactc
      181 gggatcgatc atcgcgaacg ggatgcaatg aaacgccagg tcccacgccg cgtaccaggg
      241 gtattcccac ttgtcgggca tcgagatgat gttcgcgttg tttaggtgca tccattcggc
      301 gttgcgcccg ttcttgcgcg aagccggcgg cggcggcccc gcggggtcgc cattgagcca
      361 ttcgccgatg ttgtagtaga aaaactgctt gctccagatc atccccgccc acgcctgccg
      421 ctgaacgagc cgagcatcgg catcatcgat gccggtttgg atgtcgctgt agaaagcgtc
      481 cgcttcccga cggcgtcgct cgaaggtgct atcgaactcg gcaaacgccc cctccggtcc
      541 cctcgcccgg tactccggga gagggttagg gtgagggtga ttttggtcgg gagttttcag
      601 cggcatcccg agatccccca tcccccactc cgcccccggt ccactggaag ccggcgccgc
      661 cgtccgcatc aatcgcagcc gcacccgctg cgacccctgc gccggcacgc tcaacacgca
      721 cagcgccgcc gccttcgtcc cactgcggtt cggattcacc gccgcaacgt tgccgccaag
      781 gacgaactcg tgaaacgcgt ctttgtaata cgccccatcc acatgcggca gcccaaacaa
      841 cttcggcccg ttcgtctcgt tctccgtgaa cagcagttgc ggcccaccat cggcgaacaa
      901 cgcatactgc cccaactcca catgctcgat ctcgatcgga ccctcatcac cacccgcgcg
      961 catcaccggc ttaggagacc ccgcgttcca cgaccagctg ttgcggaacc acatttgcgg
     1021 cagcaggtgc agcggcgcgt ccttcggccc gcggttgtgg accgtgatct gaatcaggat
     1081 gtcattctcg cccgccttgg cgtattcgac gaagacatca aaatatcgcc catcgttgaa
     1141 cacaccggta tcgatcaact cgaactccgg cgcgctcttg ccacgataac ggttctcctc
     1201 caccaaccgc gtgtatgggt actccgcctg cggatacttg tagagcatct tcaaatacga
     1261 atgcgtcggt gtggcgtcca ggtagtaata cagctccttc acgtcctcgc cgtgattacc
     1321 ttcaggaccg gtcaggccga acaggcgctc tttcaagatc gaatctttcc cgttccacat
     1381 cgccaccgcg aaacacagcc gctgctcgtt gtcgctgatg ccggcgatgc cgtcttcgcc
     1441 ccagcgatac gcccggctgc gcgcctggtc gtgcgagaag tgcgtccaag ctgaaccgtc
     1501 ggcgctgtaa tcttcgcgca cggtgcccca ctgccgttcg ctgagatacg gcccccaccg
     1561 ccgccaattc acatggctca ggcgatcctc gtctagtcgc ttctgctccg cgtcagtcgt
     1621 catggtgtga ttccgcatgt gcatggccgc gcgcctccgc gtgaaagcgc ccgcgccttg
     1681 agatgctgtg ggaatatacc gagttacggc cccgagtgat cagataatgt tcatcactgc
     1741 ctgtggctgc cagaccgaac gcaaataagt gccgcgcgct ctggtcccct cgcccggtac
     1801 tccgggtgaa ggtcagggtg agggtgattt cgaggacggg cgtcgcgcga aatccaaaat
     1861 caccctcacc ccagccctct ccctgagtac ccggagaggg ggaagagcca catgcgattg
     1921 ctctgctgtg cctgccccgc agagcagatc cgaccgccgc caggctcggc tcagtgctgc
     1981 gaacgtcgcg tgtgcagcgc aatgcttcgc gtgtaccatg ccgcccgctc atgcccaacg
     2041 cgcactggct gccgccggaa tggttcgtcg tgtacatcac gatcgcgtgg tgcattcgcg
     2101 cggcgatggt gccggtcgtg ctgcggcgcg agttcgcgcc cggcgccgcg ctcgcctggc
     2161 tcggcatcgt ctttctccat ccgtacattg ggctgacgct ctacatgatc gtgggtgaaa
     2221 cgcgcctcgg tccgaggcga gccgaagcgc accgagcgtt ggtccgccga tcgcgcccgc
     2281 ttcgccgcac ggacgatgcc catacacatc gcgttgaact gccggcgctg cagcattcct
     2341 acgacacgat ggtgctgcag gcggagaaga tcagcgggat gccgatcgtc ggcgggaacc
     2401 aagtcgattt catcggcgaa tccaccactt tgatcgacgg tcttgtcgcc gacattgacg
     2461 cggcgacaac tgaagtccac ctgctctact tcatcttcgc ctgcgacaag accggcggcc
     2521 gcgtcgccgc cgccgtcgag gccgcggcgc ggcgcggggt gaagtgccgg ctgctcgttg
     2581 atgcggtggc gtcgcgatac ctgctgcatc accgaggcct gtcgaagcgc ctgcgcgaag
     2641 ccggcgtggt gctggcggcg gcgctgccgg tggcgccgat cagccggcag ttgccgcgga
     2701 tggatttgcg caatcatcgc aagctggccg tcatcgacgg tcgcgtcgcg ttcaccggca
     2761 gtcataacct gatcaatccc gactacggcc gcacgcgctg cgggccgtgg gtcgatctgt
     2821 ccgctcgctt cagcggcccc gtcgtcgccg agctggcggc ggtgttcaac gaagactggg
     2881 cattcgagac cggcgaagaa ttgccgctgc cgacgatcga ccagagcgtg ccgatggacg
     2941 atggcgtgcc ggcgcaggtc gtaccgacgg gaccaagtcc gccgggcgat acctttcgcc
     3001 gcgtgttgct ggcggcggtg cagtcggcgc ggaagcggct gacgctcacc acgccatact
     3061 tcgtgccgga tgaaccgacg ctggtcgcgc tgcagatggc cgccgaccgc ggcgtcgagg
     3121 tgacgctgat cgttccgcag cacccggatc accccttcac cgccgccgcg gggcgggcgc
     3181 agtatgggcg gctcatgcag gccggcgtgg ccatctggca atatcaaccg ggcttgattc
     3241 acgcgaagac gacgacggtg gaccacgcgt tcgcggtgct ggggtcggcc aacctggatg
     3301 tgcgcagctt caacctaaac ttcgagctga gcgtgctgtt gtacggcccc gacgtgaccg
     3361 agcgcctgcg cgcgattcag atgaagtacc tggccgactc gcaccggatc gatcccgaaa
     3421 catggtcgcg acgtccggtg gtgaagcaat acgcggagcg cgcggtggcg ctgctgtcgc
     3481 cgttgttgtg attagccgct tgcggctccg cagacggaca agccgcggtc acttggtctg
     3541 ccaagccgca agcggcaggg gttctacttc tccgcggccg ggtcgatttt aaagacgaac
     3601 gtatcccctg ccggtttctc cgcgggcagg gtgatgttca agccgtcatc gctgcgatcc
     3661 gcctgtagcg ctgcatccgc gcccagcaga tgcaccgcgc cgacatcgtg cgtgtacttg
     3721 tccgatccct tcgccaacga cttgatcgtc agcttgccat cgctcggcca gccgaaggcg
     3781 atggcgtaca gcgtgtcgcc cttggtcgtg aagcggatgt ccttcgcggt gaacccggcc
     3841 ttgccttcgt tgaagttcgg cacctttttg cccggctgtg ttgacggcgt tttgggttca
     3901 gcggatgggc cttcgccgaa ggtgacccac gggcgcgtgc cgaagatcgc ttctttgttg
     3961 acgtccatcc acgatgcgat gccttcgacg accttgagct cgtcatcgtc gatcgtgccg
     4021 tcgccacgca cggggatgtt cagcagcagg ttgccgttct tgctgacgat gtcggccagc
     4081 atctggatga cggtcttggc ggtcttgtag cgatggcggt cgtagatgct gcggtcgtaa
     4141 tgccagctgc cgatgcacgt gtcggtctgc caggcaaacg gctcgatctt gtcggcgagc
     4201 ccgcgctcga tgtcccacac caggcattgc cgctgctctt cgttcaggcc cttggtgttc
     4261 atcaccgctt cgttccggcc atcgtgctgc gcgctgttcg agttgtaata atgggccgcg
     4321 atgcgcaggc cgacgtcgct gacgttgttc aacggcagca ccgtgtcgtc gaagtacagc
     4381 aggtccgggt gatacttgtt gatcaggtca accgtgcggt tgtagaactt ttcgcagtac
     4441 gccttgtccg gcacgctgct gccctggtca gcttgccaat cccaaaccag cttcttcccc
     4501 ggcgtgtgat tctgcgcgta caggtcctgc gggtcgtacc cttcccacca cgtgcccttg
     4561 ccgtcggcct tggtcagctt gccgtcgtaa ggaacgccgg cgagcgggcc ggtcttgtcc
     4621 gcgccttggg cgacttcaaa ccagctccac gcgtgggctg cgtgcacggt cacaccgaag
     4681 cgcaaccccg ccgcccgggc cgccttttcc cacgtgccga cgatgtcctt cttcggcccg
     4741 atgttcacgc tgttccacgg ctgatacttc gaatcgaagc aatcgaaatt gtcgtgatgg
     4801 ttcgccagcg cgacgaaata ctgcgccccc gcgcgcttgt acaacgcgat caacttttcc
     4861 ggttcccagt gctcggcgtg ccacaggttg tcgatgtcct tgaaccccac cttgctcgga
     4921 tggccgtaat gctcgacctg atatttgtag tcgtgctgcc cctcgatata catgtttcgc
     4981 gcgtaccaat cgccctgctc cggcacgcac tgcgcggtcc agtgcgccca gatgccgaac
     5041 tttgcatccg aaaaccacac cggcgtcttg tactgcttga gcgagtccca gctcggttga
     5101 aacggccccg ccgccatcgg ttcgctgggc gccgtggttt cgtccgctcg cgctgtttgg
     5161 attgaactga aaagagacaa cgacaaggcc gccgccgcgg ccaacgcgaa gtgctgcatg
     5221 tggctcaccc gatcaccagg ttgtcgcgat gcacgacctc gtcgtacgcc gattccgcca
     5281 gcgctgcccg cacgtccgcc gtgcgttttc ccttgatccg ctcgatatcc gctgccgcat
     5341 aattcgacaa cccgcgcgcg atgacctgac cgtcgccatc ggcgatcgct acgatatcgc
     5401 ctcgcgcgaa cgtcccgtca accttgacga tccccgccgg cagcaggctg cggtttttct
     5461 cgaccagcgc tgtgaccgcg ccggcatcga cgacgatcgt gccggatgga cgggccgccc
     5521 cgatccaccg gctgcggctg gacatctttc gagtgcccgg cacgaacagc gtgccgactt
     5581 cgtcgccggc gacgatgcgc gccagcacgt tttccatgcg accgtcggcg accaccatcg
     5641 cggaccccgc atccgtcacc atgcgtgccg cgttcacctt cgagtccatc ccacctttgc
     5701 cgagagcgga tttttctgtc cgcaccagct cgcgcgcggc atcgacgctt tcgaccaggc
     5761 gcaccgattt cccagaagta tcgagcacgc catcgacgac gctgagcagg acaagaagat
     5821 cggctcgaag cgcgtgcgcc actagcgccg caagaatgtc gttgtcgccg aacgtgatct
     5881 tcaccagctc atcggtgctg atggcgtcgt tctcgttgat gatgggcaca ccgcccagct
     5941 cgtgtaccgc gtggatcgtg ttacggacgt tgagaaatcg cgtgcgatca tcaatatcct
     6001 cgcgcgtcag cagcacctgg gcgacgtgaa tcgcgtgcgg ctcaaacgcg tccgcccacg
     6061 cgtccatgag cttccgctgg ccgatggcgg cgacggcttg cagtttcgcc agatcggtcg
     6121 ggcgcttcgg cagcttcaat tcacccacgc ccgcgccgat ggcgccggag ctgacgatgg
     6181 tgacgagcac gccccggacg cgcagcgacg ccacttgttt ggcgatggtc gccagaaacg
     6241 gcgcgtcgag ccggccggct ttgtcggtga gcagttgcgt ccccagcttc acgacgagca
     6301 gccgggcgtt ggataaatat tgagttcggg tggattccat ggcctgcgcg acagtgtatc
     6361 agcagaccat ggctttatcc caagcgcagc agctgccgct tgcggcttcg cagaccaagc
     6421 ccgcagggga ttgcgaagcc gcaagcggct gcctttgtgc tttacgaaat cctccgctcc
     6481 ccaaacagcg cgcccgtcaa gttctgcccc gccttcagcg tgatgtgctg ccccatatcg
     6541 ccagtcggca gggtctgcgt ccatcccgtc gggcggatca cgcgaacgat gtgatacccc
     6601 gcggcaacgt tggtcagctt gtagttcccg ctggcgtcgg tggtcacgta cttctcggcg
     6661 gcgtcgaact ggtgattgtt gttcgcatcg atgtacacct tccagcccga caacgcgcct
     6721 tcgccgctgt cgcgcttcag gttcgcgttc acatcattga agacggtgcc gctgatcgat
     6781 cccattggcg ccggagccgg cgcgggggcg ggcgcggggg cgccgccgct gccaaggcca
     6841 aatccttgcc gcacgtacgt gatgaccgcg tcggcggtgt aggcggcctg ccagttgtcg
     6901 gtcgattgct ggtcggcctg cgccaggaac gcatcgagcg ttgcggcccc gccgagtgtc
     6961 gcgttgtacg tcgccgccgt gcgattgcgg tcggggtatg acttgacgac gttcgtcgcg
     7021 ctcgactcaa tcgccttctg ccagccgctg atcgacatgt tcgtcgagcc gatcgcgaac
     7081 tgatttccac tggtcatgct cgacgtgcag taattgttcc cgctccagtg ctcggcggac
     7141 gaattgaacg cgttgctgtg ccgcgtgacg acgctcagcg actgctcgaa atcgttgccg
     7201 gtgatggtga ggtcattcag ggccttaagt ccgctgccgc catcgacgaa accgccgctg
     7261 atcgtcagcg cgttccgcca gccgccggtg atgttgttgg cgatcgtgac atcattgatg
     7321 ccgacgcagc tgccggggtt gttggtgatg ctcgcgtagg tcaggctgat ggccgccgcg
     7381 ttctgctgcg tgtcgtgagt gaagatattg ctggagatcg tgaagccgga gttcggcttg
     7441 atgttgccga cttcgatgcc gatgcccatt ttccccgcgg cgatgccctt atcatcaatg
     7501 aagacgttgc cggtgatgcg cccggtcaca ccaccgggcg tgctgtcggc gccgttggcg
     7561 ttgccgtagg aaatgccgac ggcgttgtgc aggaaaagat tgttctcaat gacccccccg
     7621 ccgcgggcga gcacgccggt gctcgatgcg tcggcgatga tgttgccgcg cacggtcatg
     7681 ttcagcgcgc tggcggaata gtagatgttg tggttgtaac cggtgtcgcc ggcgccgctg
     7741 atctgctggt tccagccgtt gtgatcgaag acgttctggt ccagcgtgat gccgctggca
     7801 ttcatcgaat aaaaaccctg cgaccgatcg gcggtgcaat acgcgtcggc aatgacgcac
     7861 cggcggacgg tcatgttcga cacgccctcc agcaccacgt tgtccttgaa aaagcggaac
     7921 gagcaatctt cgatcgtgac gttgctgccc gggccggtga acagcaggcc cgtcggccgg
     7981 ctgccgatcg tcgctgaatt gaacgtgcgc gagttcgggt cgcgctggtc ggcgtataaa
     8041 ttgacgccgg ttaacaccag gttgttgacg gcgtggccgg tggtcgcgct gaatccaccg
     8101 gatgcaccgg tgtcgatcgt cgggcgattg ccgctgccgt aagtgccgat gaccatcggc
     8161 tccgatgaac ttcggccgga cttgttccac gtggggaacg acgacccgct ccacgtgtcg
     8221 ccgcgtttca gcagcatcca gtcgggcgac ccgtcgcgca gcagcgaagc cgccttgctc
     8281 agcgacttca ccggcgagct tgccgacaag cccgtgttgg catcggagcc ggtcgagttg
     8341 ctgacgtaaa tcgtccgcgt gtcgctgctc ggcgtcacct gcgtccaccc cgcggtgttg
     8401 aggccgaccg aaaacagctg gcgggattcg agaacttcaa acgagaccga gacatcggcc
     8461 gtgtgggcac gggtattcat cactctgctt ctccagaaac ggattgaggc cagaggcccg
     8521 gcgagaacga aggcgcacca cgaccgcagt cgcgcgccgc gacggtttga acaacgttgg
     8581 ttgtgactaa ccagaagccc gcgaaggaca gcccggcagc accgaatgga ctaactaagg
     8641 ttcagtacga tcaggtgcaa ccgcggattg gcaggaaaaa aagaaaaagt gcgcgggagc
     8701 gctgatggat cgaatgcgga atgatgaatg cagagtgcag aatggaagag agatcgaccg
     8761 ttcatcacgg cgcatcgccg gactctattc gcgccgtcgc tgacggctct tggcggatgc
     8821 gatagagatc attgtcgtcc acgcgcgttt cgaattggta gtgggccgac aggtactgct
     8881 tgaactgccg ccaggcgagg taggtgttcc ggctgcgcac gggccgcagg cccaggatcg
     8941 ggccggtcaa catttccgcc atcgcgtcat ccgcgtcgta cgccagcacg atgtactttg
     9001 gctttcgctc gtcaaaatcc gccagcatcc ggctgcaata atcctgcggg gcgttgtcgt
     9061 aattcaccca gtaataaaag atgccatagc gggaacccgg ccgaaggccg gtctcaagga
     9121 gaattcgccc cgcctggtcg gcgaacaccg cgtcgccggg tttggtgtgg tcgatcaaat
     9181 attggctgat cgccttgtgc tgcgtcccct cattcgcgcg aacgagcttg ctgccgggcc
     9241 agtgcatcga catgagcgcg atcgggatca acccgagccc gatcaccggc gcccgcgccg
     9301 tgcgcggcag gacgccgtac aacagcgcca gcggcggcgc cagcggcagg aagtgataca
     9361 ggtacatccg cccctgcatg atgatgccgg ccagctccag cgcaaaccac gcgatgacaa
     9421 acgcaaacat cgcctgcgat ggaccgatcc gcctttcaac cggcacgaca tcgcggcgga
     9481 agacccagcc gcgaaccacc agcggctgcg cgcacatcac cccggcgatg aacagcttgg
     9541 tcaccgtgaa cgaatccatc ggcgacttgc tgccgtacca cgcgatctgc cgaaacaggt
     9601 tcggcatctc gccggcgatg ccggtcttcc acacccagta cgcgaccgcc aacgtcggca
     9661 cggccacgcc cgcgatgacg gcggccatgc aggccatgac cgtgcgccag ccgcgcgagt
     9721 gcacttgcag cagcagcgcc aaacctaagc cgccaccaac ggccagcgcc gttggcttca
     9781 ccatcgccgc agatcccgcg gcgaggccgg cgacgaacgc gtcggccagc aaagacccgg
     9841 tggtggcaat gccgccgccg atcacttcca acgccgccgc cgcggcgagg catgtgaaga
     9901 agcactggat cgtctcgagc tgaaatcccg tgtatgtcgc ccagccgaag ttgagcagcg
     9961 tgatggcggc cagaaaggtc gccaggtgcg catgatcccc cgcgtgtcgc cgggccgcgc
    10021 ggg
//
LOCUS       DATIMH010001210         5109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4039511, whole genome shotgun sequence.
ACCESSION   DATIMH010001210 DATIMH010000000
VERSION     DATIMH010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4039511"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VLI90_20900"
     CDS             complement(<1..421)
                     /locus_tag="VLI90_20900"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSV16737.1"
                     /translation="MSKQNPTAKTTLKPNDLIVIAGGGGFIGGALARYFTEQGFTRIR
                     AVDKKPLPDWYQHVPGVESLCLDVSDPTNCERACEGAVEVYNLAADMGGMGFIERFRV
                     ECLRSILINTNLIEAAYRAGCERYFYASSACAYNTKLQ"
     gene            710..2716
                     /gene="pheT"
                     /locus_tag="VLI90_20905"
     CDS             710..2716
                     /gene="pheT"
                     /locus_tag="VLI90_20905"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSV16738.1"
                     /translation="MRTSLEWLSQYLPGPLDGQTAADALTNGGLPVEVIEQVGDDTVI
                     DVEVTSNRPDCLSHLGVARELSALLNREFQDLIISELPGDGPTGVTALAIDAPDLCPH
                     YTARIIRNVTIAPSPAWMVRRLEAVGLRPINNIVDVTNYVLFELGQPLHAFDFDKIDG
                     RQIVVRRAKPGEKIVSIDGHERTLTPNMLVIADASRPVALAGVMGGRDTEVSGATVNI
                     LLESARFDPLSVRGTARALAMASDSSYRFERGIDPTLPERASRRATQLILETAGGEIA
                     GPLLAAGASGYSPKQLWLRLARLTQVLGTEFPTQIVVDAFIRLRLSPVLRGERIDVTI
                     PSHRLDLNLEIDLIEEAARVVGYDKVPVRDEISIRLAPPNLLLKASETIRNTLVAGGY
                     FEAVTFSFVSDALANAFVPPEAGSLPRADARVRKADASLRPSILPGLLEAIRRNESNG
                     VAGAKLFEIGSTFWNDKAGQLQEHRRVALVGGTDYRDARGVIETLLLELDADRAIRIT
                     PADRPGYARGACGKIEWGSESIGYIGKIDRAVADQLSLRELPLAAELDLAPLLSGAQH
                     VPQLHALPRYPAVRRDLSLVLPETTRYEQLESTVRAAKLTDLEAIEYVTTYRGKPLDA
                     GHKSVTITLIFRSTTTTLTSEAVEDAVQRVVNAAKQTLGGTLRT"
     gene            complement(2979..3434)
                     /gene="tsaE"
                     /locus_tag="VLI90_20910"
     CDS             complement(2979..3434)
                     /gene="tsaE"
                     /locus_tag="VLI90_20910"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSV16739.1"
                     /translation="MSDLTYTTASVAETEAVAATIARECRGGEVIALSGDLGAGKTQF
                     VRGLVRALGGNPRAVSSPTFVLLNVYDGGRFTIYHLDAYRVGGADDFEAIGFTELLEQ
                     GGVVAVEWPERVADLLPTRIWRVRLTALDETRRLIEVLPPAPLPRAAEG"
     gene            complement(3431..>5109)
                     /locus_tag="VLI90_20915"
     CDS             complement(3431..>5109)
                     /locus_tag="VLI90_20915"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSV16740.1"
                     /translation="TPGRPPYRLYALSNVASFLALLSYPTLVEPTMTRRMQALVWSIC
                     LAVFAVVCAYCAINSTTPGSERNRDDPDSSARRAAESGSSAYLRNGRDDIWWLLLPAG
                     ASLLLLAVTNKICQDVAVVPLLWVLPLAIYLLSFVICFDSPRWYDRRVFVPLLLIALA
                     IACWAAFPGHEEWSIPLRVAAYALALFACCMTCHGELYRLKPPPARLTAFYLLIAAGG
                     ALGGLLVALVAPMIFTGYSELQIGLMLAFTLLLVALFRDPKSLIYHGRRPVLWLAMII
                     AGVALALLLWITATPHLAGSRMLAQMRNFYGVLTLYRQGSGEAETVLLQHGGVTHGLQ
                     LTTPSLRRQPTAYYGPQSGVGVALRSLPALQPHRVGAIGLGAGTIAAYARAGDGYRFY
                     EINPMVIDIARSRFTFLGDCLGHVEVVPGDGRLSLENEPDRHFDLLAVDAFSGDAIPV
                     HLLTAECFEVYLRRLAPRGIIAIHVSNQHLDLEPVVRKLAAHAGLACVLVHATPVNDP
                     FQYESRWMLLARDAASLDVEPIKSAVSPAEARREVPLWTDDYSDLIRILR"
BASE COUNT          965 a         1788 c         1561 g          795 t
ORIGIN      
        1 cctgcagctt tgtgttgtaa gcacatgcgg atgaggcgta gaagtaccgt tcgcagccgg
       61 cgcgatacgc cgcttcgatc aggttcgtgt tgatcagaat cgaccgcagg cactcgacgc
      121 ggaagcgctc gatgaacccc atgccgccca tgtcggcggc caggttgtag acctcgaccg
      181 cgccttcgca ggcgcgctcg cagttcgtcg gatcgctcac gtccaggcac agcgactcga
      241 cgcccggcac gtgctgatac caatctggca gcggcttctt atcgaccgcg cgaatgcgcg
      301 taaaaccctg ctcggtgaag tatcgcgcca acgccccacc gatgaacccg ccgcccccgg
      361 cgatcacgat caggtcattc ggcttcagcg tcgtcttggc ggtcggattt tgcttgctca
      421 ttctccgctt tctccgtatc tggcaatctg catgaacccc cggaacagcc cgccgcggct
      481 aacgcgcggc aacgcccatt ctctctatac gccacccccc gatgcaagga tcggtcgcag
      541 gcgtttgaca ccccctcgcc cgcccctaaa gtgtcaccgt ttgtaacggc tgtaacggag
      601 atgtcagggt tcaggggtca gggttcagaa aaggctggac ccggttttcg acgcctgtcg
      661 cctgacttct gccccctgaa ccttgacccc tgacccctga accctcccca tgcgaaccag
      721 ccttgaatgg ctcagccagt acctgccggg cccgctcgac ggccagaccg ccgccgatgc
      781 actaaccaac ggcggactcc ccgttgaagt gatcgagcaa gtcggcgacg acaccgtcat
      841 cgacgtggaa gtcaccagca atcgccccga ttgcctgtcg cacctcggcg tcgcccgcga
      901 attgtccgca ctgctcaatc gcgaatttca agatctgatc atttcagaac tgcccggcga
      961 tggacccacg ggagtgaccg cgctggccat cgacgcccct gatctctgcc cgcactacac
     1021 cgcgcgcatc atccgcaatg tgacgatcgc ccccagcccc gcgtggatgg ttcgccggct
     1081 tgaagcagtc ggccttcgcc cgatcaacaa catcgttgac gtcaccaact acgtcctgtt
     1141 cgagctcggc caaccgctgc acgcgttcga cttcgacaag atcgatggtc ggcagatcgt
     1201 cgtccgccgc gccaagcccg gcgagaagat cgtcagcatc gacggccacg agcgcacgct
     1261 gacaccgaac atgctcgtca tcgcggatgc atcccgacct gtcgcgctcg caggcgtcat
     1321 gggtgggcgc gacactgaag tctcgggcgc caccgtgaac atcctgctcg aatcggcccg
     1381 cttcgatccg ctcagcgtcc gcggcaccgc ccgcgcgttg gccatggcca gcgacagcag
     1441 ctaccgcttc gaacgcggca tcgatcccac gctgccggag cgcgccagcc ggcgggcaac
     1501 gcagctcatc ctagaaaccg ccggtggtga aatcgccggc ccactgctgg ccgcgggcgc
     1561 ttcgggctat tcgccaaagc aactttggct tcggttggcc cgactgacgc aggtgctcgg
     1621 caccgagttc cccacgcaaa tcgtggtgga cgccttcatc cgcctccggc tgtcgcccgt
     1681 gctccgcggc gaacgcatcg acgtcaccat cccaagccat cggctcgacc tgaacctcga
     1741 aatcgatctc atcgaagaag ccgcacgcgt cgtcggctac gacaaggttc ccgtgcgcga
     1801 cgagatttcg attcgcctcg cgccgccaaa cttgctgctg aaggcgagcg agacgatccg
     1861 caatacgctc gtcgccggcg gttatttcga agccgtcacg ttcagcttcg tcagcgacgc
     1921 gttggcgaat gccttcgtcc cgcccgaagc ggggtcactc ccgcgcgccg atgcccgcgt
     1981 gcgaaaggcc gacgccagcc tccggccgag catcctgccc ggcctgctcg aggccatccg
     2041 ccgaaacgaa tccaacggcg ttgccggcgc aaaattgttc gagatcggct cgacgttctg
     2101 gaatgacaag gccgggcaac tgcaggaaca ccgccgggtc gccctggtcg gcggcaccga
     2161 ctaccgcgac gcccgcggcg tcatcgagac gctgctgctc gaactcgacg ccgaccgcgc
     2221 catccgcatc acccccgccg accgccccgg ttacgcgcgc ggcgcctgcg gcaagatcga
     2281 atggggcagc gaatcgatcg gctacatcgg caagatcgat cgcgccgtcg ccgatcaact
     2341 ctcgctgcgc gaactgccgc tcgccgcgga gctggacctc gccccgctgc tgtccggcgc
     2401 gcagcacgtc ccgcaactgc acgcgctgcc caggtacccc gcggtgcgcc gggatctctc
     2461 gctcgtgttg ccggagacga cgcgatacga gcaactggaa tccaccgtcc gcgccgccaa
     2521 gctcaccgat cttgaagcga tcgagtacgt gacgacctac cgcggcaagc cgctggacgc
     2581 ggggcacaag agcgtcacga tcacgctcat cttccgctcg accacgacaa ccctcaccag
     2641 cgaagccgtc gaagacgcgg tgcagcgcgt ggtgaatgcc gcgaaacaaa ccctgggcgg
     2701 caccctgcgc acctgaacga cgcggcacgg ctacggctac ggctgcgctt cgcgccatcg
     2761 ggagcgcgcg caccttccac gccgggttcg gaggtactcc gacgacccgg atgctgcggc
     2821 gcgcgagaag aatccgggtc gtcgcgagta cgaacccggc gtggtgcgct tcctcctcca
     2881 acggggcgga tgtcatgggg ggaaggagaa gcagcgaggg gaagaacctc ggggccgcat
     2941 gggccgtccc ctcgcccctt ctctctcgaa tctgcgcatc atccctcagc agcgcgcggc
     3001 agcggcgccg gcggcaacac ctcgatcagt cgccgcgttt catcgagcgc cgtcaaccgc
     3061 acgcgccata tccgagtcgg cagcaaatcc gccacgcgtt ccggccactc gaccgcgacc
     3121 acgccgcctt gttcgagcaa ttccgtgaag ccgatcgcct caaaatcatc cgctccgccc
     3181 acgcgatacg catccaggtg atagatcgtg aatcgaccgc cgtcgtagac gttcagcagc
     3241 acgaacgtcg gactcgacac cgcgcgcggg ttgccgccga gggcgcggac caggccgcgg
     3301 acgaattgcg ttttgccggc gccgaggtcc ccgctgagcg cgatcacttc gccgccgcgg
     3361 cattcgcggg cgatggttgc cgcgaccgcc tcagtctcgg cgacgctggc cgtggtgtac
     3421 gtgagatcgc tcatcgcagt atccggatca ggtccgagta atcatctgtc cacaatggca
     3481 cttctcgcct ggcctccgcc ggtgacacgg ccgacttgat cggctcaaca tccaacgacg
     3541 ccgcatcgcg cgccagcagc atccagcgcg attcatactg gaacggatcg ttcaccggcg
     3601 ttgcgtgcac cagcacgcac gcgagtccgg cgtgggcggc caacttgcgc acaaccggct
     3661 ccaggtccag gtgctggttg gacacgtgaa tcgcaatgat gccgcgcggc gcgaggcgcc
     3721 gcaggtagac ctcgaagcac tcggccgtca gcaggtgcac cgggatcgcg tcgccgctga
     3781 aggcatcgac ggccagcaga tcgaaatgcc ggtccggctc gttctccagc gacagccggc
     3841 catccccggg cacgacctca acgtgcccga gacaatcgcc aaggaacgtg aaccgcgagc
     3901 gggcgatgtc gatcaccatc gggttgatct cgtaaaaacg ataaccatcg cccgcccgcg
     3961 cataagccgc gatcgtgccg gctcccaggc caatcgcccc aacgcgatgc ggctgaagcg
     4021 cgggcaagga tcgcagcgca acgccgacgc cgctctgcgg gccataataa gcggtcggct
     4081 gccggcgaag cgatggcgtc gtcaactgca gcccgtgcgt gaccccgccg tgttgcagga
     4141 gcacggtttc cgcctcgccc gatccttgtc gataaagcgt gagcacacca tagaagttgc
     4201 gcatctgtgc gagcatgcgg ctgcccgcaa ggtgcggcgt cgcggtgatc cacaagagca
     4261 gcgccaatgc gacgccggcg atgatcatcg ccagccacag gacgggtcgc cggccgtggt
     4321 agatgagcga cttcgggtct cggaacagcg caaccagcag caacgtgaac gccagcatca
     4381 ggccgatctg aagttcgctg taaccggtga agatcattgg cgcgacgagg gcgacgagca
     4441 acccgccgag cgcgccgccg gcggcgatca gcaggtagaa cgcggtcagg cgcgccggtg
     4501 gtggtttgag tcgatacagt tcgccgtggc aggtcatgca gcaggcgaac agcgccagcg
     4561 cgtacgccgc gacgcgcagg gggatggacc attcttcgtg accgggaaac gccgcccagc
     4621 aggcgatcgc gagtgcgatc aagagcagcg gcacgaacac gcgacggtca taccagcgcg
     4681 ggctgtcgaa gcagatgacg aacgacagga ggtaaatcgc cagtggcagc acccacagca
     4741 gcggcacgac agccacgtcc tggcagatct tgttcgtgac ggccaacagc agcagcgatg
     4801 cgcctgcggg gagtaacaac caccagatgt catcacgccc gtttcggagg tacgccgacg
     4861 acccggactc tgcggcgcgt cgagcagaag aatccgggtc gtcgcgatta cgctccgaac
     4921 ccggcgtggt ggagttgatc gcgcagtagg cgcagacgac ggcaaagacc gcgaggcaga
     4981 tcgaccatac gagcgcctgc atgcgccgcg tcatcgtcgg ctcgaccagg gttggatacg
     5041 acagcagcgc gagaaacgat gcgacgtttg agagcgcata cagccgatac ggcggccgcc
     5101 ccggcgttc
//
LOCUS       DATIMH010001211         4248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_286837, whole genome shotgun sequence.
ACCESSION   DATIMH010001211 DATIMH010000000
VERSION     DATIMH010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286837"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="VLI90_20920"
     CDS             complement(<1..1401)
                     /locus_tag="VLI90_20920"
                     /inference="COORDINATES: protein motif:HMM:NF039502.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HSV16741.1"
                     /translation="MTHETLSIALIGTSRGGAAGIALPAIAQDAAAKLPPATPERTLL
                     NRTAILFACDACGAKPTIATERVAECPPDERPPCSPRAAEILRDLITSNAHDLIHEWL
                     NLAQVAKRRPPHRLLPALLDYAAKHPADRQRIGEVIDRRGHWLMSLNPEWQFGATADA
                     APDGVWQTGTHPQRLSVLRSLRQHEPARAREMIATSWKDEPAEQRADIVAALLVGLTA
                     DDEPFLESALDDRSKVVRAAAADLLARLPESQFVRRMIDRVTPRLVVRSRAAELEVDL
                     PQLYDATMQRDGIEEKSPGKGQKQWWLEQMLGMVPPAHWSKIWKLAPREFMSLTVGDF
                     PDSLLDGLAKACERCPDPAWIEPLLRRGLTRITPALNRSLLAVLPPARFLSFALDVLQ
                     SKAATLSLCSDLIKGRHVPLDVTTGRAFVRTVAELMNARSRQDVDILLHLIDGCAGRL
                     PPSLHDEIASKWLAERE"
     gene            complement(1529..2878)
                     /locus_tag="VLI90_20925"
     CDS             complement(1529..2878)
                     /locus_tag="VLI90_20925"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HSV16742.1"
                     /translation="MATGWNRDRVLQLAPDAASAKAGQELANARKWSSIGGDEQAIWG
                     LCQGSGKNPYQTIIDLGEPAFKCSCPSRKFPCKHGLGLLLQFAAAPEGFTRAERPAWV
                     AEWMTTRQERAQKKAEAAAQPPKPVDAEAQAERRAKRHDRVAAGLASLRTWIDDIVRD
                     GIAAVQPRGYSFFDEPARRMIDAQAPGAARMIADLGVTMSSGGDWHEPFLRQLSSLHL
                     LVEAHAKIDQLPSETREDVLARLGIAVPQEEVLATPPLQDTWQVTALEVDVDERLRVQ
                     KTWLFGVNSRRIALILHFAHDTAAFEANFAIGSQFDGEICRFPGTGIRAAVKSRGSST
                     AIEKLAGFDDLDAACDAFSQMLGRQPWLEPVALPLRNVVPTLSKNSWSVIDATGHALP
                     ATMPPGIAWQATAISGGHPIGIVATFDGQTLRPLTLFTEREVIPTTHDRAMTPFSPA"
     gene            3013..4218
                     /locus_tag="VLI90_20930"
     CDS             3013..4218
                     /locus_tag="VLI90_20930"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSV16743.1"
                     /translation="MSKPLSLALVGLGGYGNGYVSALLDHWDRGDVRLIAGVDPMPTT
                     CARLAEVQARQVPIYPSLDAFFKDATADLVLICSPIQYHASQTCLALNHGVNVLCEKP
                     LCATLDDAGEMLSARDRAGKLVAIGYQWSYSKAIGELKADILAGKLARPRRLKCIALW
                     PRDESYYTRNGWAGAKQDAQGRWVLDSPVNNACAHQLHNMLYVLGPQTDRSAKPARLT
                     AELYRANNIGNYDTAAIRCFTSDGVELMFITSHATAHSHGPVFEYEFDNATMRFHDGP
                     AAPIVAKFKNGGERNYGTPMEPRDHKLSLTLTHVREGMPTPCGIEAAVSHTQCVWAAQ
                     QSVAEITPFPRERVRVTGEPGHRKTTVEGLSETLTRCYDEWKLPSEIGAGWGQPGREV
                     HIDGMLINK"
BASE COUNT          763 a         1385 c         1349 g          751 t
ORIGIN      
        1 ttcacgctcg gcgagccact tgctggcgat ctcatcatgc agcgacggcg gtaagcggcc
       61 ggcgcatccg tcaatcaagt gcaacagaat atcgacgtcc tgcctgctcc gtgcattcat
      121 cagctcagca accgttcgga cgaaggcgcg acccgtcgtc acatccagcg gcacatggcg
      181 gcccttgatc aggtcactac acaatgacag ggttgccgct ttcgactgaa ggacatccaa
      241 cgcaaagctc aggaaccgcg ccggcggtag cacggccagc aatgatcggt tcaatgccgg
      301 cgtgatgcgc gtcaaccccc ggcggagcag cggttcgatc caggccgggt ccggacaccg
      361 ctcacaggct tttgccaggc catcgagcag cgagtctgga aaatcaccaa cggtcagcga
      421 catgaactcg cgcggcgcca gcttccaaat cttcgaccaa tgcgccggcg gcaccatgcc
      481 gagcatttgt tcaagccacc actgcttctg gcccttcccc ggcgactttt cctcgattcc
      541 atctcgctgc atggtcgcgt cgtatagctg gggcagatcc acctccagtt cggccgcgcg
      601 ggatcgaacg accagccgcg gcgtgacgcg gtcaatcatc cgccggacga actgcgattc
      661 cggcaggcgc gcgagcaggt ccgcagccgc ggcgcgaacg accttgctgc ggtcgtccag
      721 cgctgattcg aggaatggct catcatctgc ggtcagtccg accaaaagcg cggcgacgat
      781 gtcggcccgc tgctccgccg gctcgtcttt ccatgacgtc gcgatcatct cgcgggctcg
      841 cgcgggctcg tgctgtcgga gactccgcaa gacgctcagc cgctgcggat gcgtccccgt
      901 ctgccagaca ccatcggggg ccgcgtcggc agttgcgcca aattgccact cggggttgag
      961 cgacatcaac cagtgcccgc gacgatcgat gacttcgccg atgcgctggc gatccgccgg
     1021 gtgtttcgcg gcgtaatcga gcagcgccgg cagcagccgg tgcggcgggc gacgctttgc
     1081 aacctgtgcc aaattcagcc attcgtggat cagatcgtgc gcgtttgacg tgatgagatc
     1141 gcgaagaatc tccgctgccc gcggcgagca cggcgggcgt tcatccggcg ggcactcggc
     1201 gacgcgctca gtggcgatgg tcggtttcgc gccacacgca tcgcaggcga agaggatcgc
     1261 cgtgcgatta agcagcgtcc gctccggcgt cgccggcggc agtttcgccg cggcgtcctg
     1321 cgcaattgcg ggaagcgcaa ttcccgccgc gccgccgcgc gacgtgccga tcagggcgat
     1381 gctgagggtt tcatgcgtca ttgcgattcg aatcgtcgaa cactccattt agcccggcgt
     1441 gaacacacgc cgggtttact cgggtcttcg cgcatcatta tttgagaatt ccaaccggcg
     1501 cgacttctca acgtaacccg gcgtgtgttc acgccgggct aaatggggtc atcgcgcggt
     1561 cgtgtgtggt tggaatcact tccctttctg taaacagcgt cagcggccgt agcgtttggc
     1621 catcaaacgt tgcgacgatg ccgatcggat gcccgccgga aatcgcggtg gcttgccagg
     1681 cgatccccgg cggcatcgtc gccggcaggg cgtggccggt cgcatcaatc acgctccagc
     1741 tgtttttgct gagtgttggc acgacgttgc gcagcggcag tgcgaccggc tccagccacg
     1801 gctgccgccc gagcatttgg ctgaacgcat cgcatgcggc atcgaggtca tcgaagccgg
     1861 ctaatttttc gatagctgtc gacgagccgc gcgatttgac ggcggcgcgg atgccagtgc
     1921 cggggaagcg gcagatctcg ccgtcgaact gtgagccaat cgcgaaattc gcttcgaacg
     1981 ccgcggtgtc gtgggcgaaa tgaaggatga gcgcgatgcg gcgcgagttg acgccgaaga
     2041 gccacgtttt ctgcacgcgc aggcgttcgt caacgtcaac ttccaaagct gtgacctgcc
     2101 aggtgtcctg cagcggcggc gttgccagca cctcttcctg cggcacggca atgccgagcc
     2161 tggccagcac atcctcgcgc gtctccgacg gcagctggtc gatctttgcg tgggcctcga
     2221 ccagtagatg cagcgacgag agttgacgaa ggaatggctc gtgccaatca ccgccgctgg
     2281 acatcgtcac gccgaggtca gcgatcatcc gcgcggcgcc cggcgcttgt gcgtcgatca
     2341 tccgcctggc cggttcgtcg aagaacgagt aaccgcgcgg ctgcaccgcg gcgatgccgt
     2401 cgcggacgat gtcgtcgatc caggtccgca gcgacgccag ccccgccgcc acgcgatcgt
     2461 ggcgcttcgc ccgccgctcc gcctgcgctt cggcatcgac aggcttgggc ggttgcgcag
     2521 cggcttcggc ctttttctgg gcgcgctctt gccgggttgt catccactcg gcgacccacg
     2581 cgggtcgttc agcgcgagta aaaccctctg gtgctgcggc gaactgcagc agcagaccca
     2641 gaccgtgttt gcaagggaac tttcggctgg gacacgagca cttgaacgcc ggctcgccga
     2701 ggtcgatgat cgtctgatac ggattctttc cgctgccctg gcagaggccc cagatggcct
     2761 gctcgtcgcc accgatcgac gaccacttgc gggcattggc cagctcctgc ccggccttag
     2821 ccgacgccgc atccggcgcc agctgcaaca cgcgatcgcg gttccacccc gtcgccatgg
     2881 tcgcacatcc aaccgcccgc acaccgtttt gtcaagatta aatgtaagca atatgttagg
     2941 tgactgtcga gaagcgggtg ttagccgatt taccatcgcc ccggtacgat ctaagccatt
     3001 gcaggtacag cgatgagtaa acccctgtcc ctcgccttgg tcggcctcgg tggctacggc
     3061 aacggttacg tctcggcgct cctggatcac tgggatcgtg gcgatgtgcg cttgattgcc
     3121 ggcgttgacc ccatgccaac cacctgcgcg cgcctggccg aagtgcaggc ccggcaggtt
     3181 ccaatttacc catcgctcga cgcgttcttc aaagacgcga ccgccgacct ggtgctgatt
     3241 tgctcgccga tccaatacca cgccagccag acctgcctgg cgctgaacca cggcgtgaac
     3301 gtgctgtgcg agaagccgct gtgcgcgacg ctcgatgacg ctggcgaaat gctgagcgcg
     3361 cgcgatcgcg ccggcaagct ggtggcgatc ggttaccagt ggtcgtacag caaagcgatc
     3421 ggcgaactga aggcagacat actcgcggga aagctcgcgc gcccccggcg gctcaagtgc
     3481 atcgcgcttt ggccgcgcga cgagtcatac tacacccgca atggctgggc cggcgcgaag
     3541 caggatgcgc aagggcgatg ggtgctggac agcccggtga acaacgcgtg cgcccatcag
     3601 ctgcacaaca tgctctacgt gctgggtccg cagaccgatc gcagcgccaa gcccgcgcgc
     3661 ttgacggccg agctgtatcg cgccaacaac atcggcaact acgacaccgc cgccatccgc
     3721 tgtttcacca gcgatggggt ggagctgatg ttcatcacct cgcacgccac ggcccacagc
     3781 cacgggccgg tcttcgaata cgaattcgac aacgcgacga tgcggttcca cgatggcccc
     3841 gccgctccga tcgttgcgaa gttcaagaac ggcggcgagc ggaattacgg cacgccgatg
     3901 gagccgcgcg atcacaagct gtcgctgacg cttactcacg ttagggaagg catgccgacg
     3961 ccgtgcggca ttgaggccgc cgtgtcgcac acgcagtgcg tgtgggcggc gcagcagagc
     4021 gtggcggaga tcacgccgtt cccacgcgaa cgagtgcgcg tgactggcga gccggggcat
     4081 cgaaagacga ccgtggaggg gttaagcgaa acgctgacgc gctgctacga cgagtggaag
     4141 ctcccgagcg agatcggcgc cggctgggga cagcccgggc gcgaagttca catcgatggg
     4201 atgctgatca ataaatgagc cgcgcggcgt ggttgccaaa gcaaccac
//
LOCUS       DATIMH010001212         7532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_286873, whole genome shotgun sequence.
ACCESSION   DATIMH010001212 DATIMH010000000
VERSION     DATIMH010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286873"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VLI90_20935"
     CDS             complement(<1..908)
                     /locus_tag="VLI90_20935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSV16744.1"
                     /translation="MPSAPSCAIVIFGASGDLAKRKLIPALYELARLKLLDERSYIVG
                     YSRSPMSDDEFRKEARDAVTKFARTKPVDEAVLKKLEGRLFYHAGDYGSEESHAACSE
                     RMAQLDRQFGTESNRLFYISTPPQAFEPIITCLGKRQQANPNQGRWKRIIIEKPFGHD
                     LQSARMLNDLLHRNFQEEQVFRIDHYLGKETVQNLMVMRFANSIFEPIWNYKYIDHVQ
                     ITVSETLGVEKRGGYYDKSGALRDMVQNHLFQLMALVAMEPPAALDAVSIRDEKVKVY
                     KSVRPFDAERLSAFVVRGQYGPGQANG"
     gene            complement(1083..1571)
                     /locus_tag="VLI90_20940"
     CDS             complement(1083..1571)
                     /locus_tag="VLI90_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16745.1"
                     /translation="MVVVKGNSMACGSAACGFAGHIARIAKAQAAVSEKSEDSEPLPI
                     LLAGAPVASPYDVDRLDFREVNMFRLLKLAAYALLGYVLYELFLGISEGEPGRALSGG
                     GGQRQRGGQRQGGGQQSGGGAGGSDQGVTMSGPGEGRTIPVTDIGGAERNERVGRGVV
                     TG"
     gene            1598..2146
                     /locus_tag="VLI90_20945"
     CDS             1598..2146
                     /locus_tag="VLI90_20945"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSV16746.1"
                     /translation="MSTFVSRGGQKLHHAIAAFAIDVHAKTCADLGCSTGGFTDCLLQ
                     RGAAKVYAVDTGYGVLDWRLRNDPRVVVMERTNAMHVQLPEPVEIVTIDVAWTKQRHI
                     LPSGKRLLAPGGIVITLIKPHYEAAPPLLRKGILPEDQIPAVLESVKDDVQHAGFTVA
                     AMVESPIKGAKGNVEMLAMLTM"
     gene            complement(2199..3428)
                     /locus_tag="VLI90_20950"
     CDS             complement(2199..3428)
                     /locus_tag="VLI90_20950"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HSV16747.1"
                     /translation="MATTSQVDEKQVSPAGRSNGQNPKAVAEATLAAVKEQTHPMTSF
                     VESCLETVADSAVAAERKSRGRPRAPRLLRVLAGKRNILVTTHLHPDPDALASSMAMV
                     QLLRAKLPAGTRVSMSIKGKVASGINAKFANLSDLELVPWDDERLKEYDGFVLLDTQP
                     QSSFSPLPKTIETDVVIDHHQGKKHTRPHCPFCDIRTEVGATSSMVFSYFMELEVQIS
                     PALAASLLFAIESDLAGASGTPGELDNIALSSLTLMADTHRLYQMRYVDLPQSYYIAY
                     ASALGHAVYYDNALMSHIDEIDSLEKPAVMADFLLRFDQVQWALVTAVHENRLILSLR
                     TSSKAMSAADMIRKLLRNWGEGGGHRTKAGGVIPLSNGSPTEVARLRDVLRRRYLSAL
                     KIRATRGQRLVPKSNNS"
     gene            3688..5187
                     /locus_tag="VLI90_20955"
     CDS             3688..5187
                     /locus_tag="VLI90_20955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16748.1"
                     /translation="MSLAIDVETGTDEVRTDRHIVPVLVAIAIFAGLSLWAAIKSDGF
                     LEADSCTHYIYARYAFAQPHLFVNIWGRPVCTGIYSIGAHFAGRMGVRVTSLIIAIAI
                     ALIVMQIARGMQWGATAPTLALIFTLAQPLVFLHSFSELTELPFALLVALGFWAYQRQ
                     RWLWFALAIGMTPLSRPEGFGLLALAAVALLLHRRWSWLALLALPLVAWDYCGWVEYG
                     RVGPWWNWLRANWPYEQKSLYTPGPLYHFLLLMPVVTSPLIFPAMVVGAWRCLTPDLR
                     KKRQDAADPEHLQRCNAIIALLPLSILVGHSLLYWLGKMASNGELRYLLVVAPFWALL
                     AARGWSWVFQETRWQHAVAWAAAAALAPVMIHLTYPVLPLHQQPDWVEAQQIAQWYQD
                     SSHDHHPYLATAHPGILYHLDLSPSDGSRVREWRKDVLDAVPPGTTVVWDPIYGVFNS
                     DKSRSISAEELLNAGWKEVQMPWGGSAAAGKWRVFQSQPITAKSKSPLP"
     gene            complement(5378..6505)
                     /locus_tag="VLI90_20960"
     CDS             complement(5378..6505)
                     /locus_tag="VLI90_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HSV16749.1"
                     /translation="MSCIKIAIAGVGNCASSLVQGIAFYAAHPRDVGLQHPDLGGYRV
                     SDLDVVAAFDIDARKVGRSLQAACFAAPNNTKVFQPELPAGGVIVQKGPTLDGCPPHM
                     LEFPPHMRFEPASLPAVNVAEVLRDSGAEILINYLPVGSQRATEHYAQACLDAGVALV
                     NCIPVFVASDPAWARRFQERGLPIIGDDIKAQLGATITHRTLARLFHDRGVRIDSTYQ
                     LNTAGNTDFLNMLARDRLASKRISKTEAVQSQLDVPLDSEQIHIGPSDFVPFQKDNKV
                     CFLRIEGTGFAGVPMHVELRLSVEDSPNSAGVVVDAVRCCRLALDAGMSGPINAAAAW
                     TMKHPPRQMIDHDARLAMEAFIVDAMSQRRPRLVPSPGTPG"
     gene            6938..7330
                     /locus_tag="VLI90_20965"
     CDS             6938..7330
                     /locus_tag="VLI90_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16750.1"
                     /translation="MKRLFRLTVLALLVGGWALAGSALHIVRTPGHVIVLPKNRLTFH
                     DTYVDTRAWTIQNVRQHPDVTARIVQVGKGNLLSHAVENSTGDVPAQLADAIAHPLAA
                     PPTTRPAIVDKAMAEVHAATQSVRAVFD"
BASE COUNT         1344 a         2347 c         2401 g         1440 t
ORIGIN      
        1 ccgttggctt ggcccgggcc gtattgaccg cggacgacga acgcgctcaa gcgctcggcg
       61 tcgaaggggc gaacgctctt gtagaccttc accttctcgt cgcgaatgct gaccgcatcc
      121 agcgctgccg ggggttccat cgcgaccagc gccatcagct ggaacaggtg gttctgcacc
      181 atgtcgcgca gcgcgccgct tttgtcgtaa tacccgccgc gtttttccac gccgagcgtt
      241 tcgctgaccg tgatctgcac gtgatcgatg tacttgtaat tccagatcgg ctcgaagatc
      301 gagttggcga agcgcatcac catcaggttc tgcacggtct ctttgccaag gtaatgatcg
      361 atgcggaaga cttgctcttc ctggaaattc cgatgcagca ggtcgttcaa catgcgggcc
      421 gactgcagat cgtggccgaa cggtttttcg atgatgatcc gcttccaccg gccctggttg
      481 ggattggcct gctggcgctt gccgaggcag gtgatgatcg gctcgaacgc ctgcggcggt
      541 gttgagatgt agaacagccg attcgattcc gtgccgaatt gccggtccag ctgcgccatc
      601 cgctcgctgc acgcggcatg gctctcttcc gagccgtagt cccccgcgtg atagaacagc
      661 cgcccttcca gttttttcaa caccgcttca tcaaccggct tggtgcgggc gaacttcgtc
      721 accgcgtcgc gcgcctcttt gcggaactca tcatcgctca tcggcgaacg tgagtagccg
      781 acgatgtacg agcgctcgtc cagcagcttc aggcgcgcca gctcgtagag cgcggggatg
      841 agtttgcgct tggcgaggtc gccgctggcg ccgaagatca cgatcgcaca cgatggggcg
      901 gacggcatac gcgttctcct tcacaagaac gcgcacagaa atacgcgggg aaatcggcaa
      961 atgcaatcac gtgcaaatgg aactacgagg cctgcgtcgc cttggcgctc aagggcgacg
     1021 caggcgtcgc aactacagga ttcgaaaagg atggagcacg gcgcgcccgc gcacgcctca
     1081 catcagccgg tcacaacgcc gcggccgacg cgctcattgc gttcggcgcc gccgatgtcg
     1141 gtgaccggaa tcgtgcggcc ttcaccgggg ccgctcatgg tgacgccttg atcactaccg
     1201 ccagcgccgc caccggattg ctggccgccg ccttgtcgct ggccaccacg ctgccgctga
     1261 ccgccgccgc cggacagcgc gcggccgggt tcaccttcgc tgatgccgag gaacagttcg
     1321 tagagcacgt aaccgagcaa agcgtacgct gcgagtttca acagacggaa catattgacc
     1381 tcccgaaaat ctaaacgatc aacatcgtac ggcgaagcaa ctggtgcgcc agccagcaaa
     1441 atgggcaagg gttccgagtc ttcagatttc tccgataccg ccgcttgcgc ctttgcaatc
     1501 cgggcgatgt ggcctgcgaa gccgcaagcg gcggaaccgc acgccatcga gttgcccttc
     1561 acgacaacca agccgcgcgg cgatacgctg tggcgaaatg tcaacttttg tctcccgcgg
     1621 cggtcaaaaa ctccatcacg cgatcgcggc gtttgcgatc gacgtgcatg cgaaaacctg
     1681 cgccgatctc ggctgtagca ccggcgggtt taccgactgc ttgctgcagc gcggcgctgc
     1741 caaggtgtac gccgtcgaca ccggctatgg cgttctagac tggagactgc gcaacgaccc
     1801 gcgggttgtc gtgatggaac gcacgaacgc gatgcacgtg caactgcccg agccggtcga
     1861 gatcgtcacg atcgacgtcg catggacgaa gcagcggcac atcctgccat cggggaagcg
     1921 actgctcgcg ccgggcggga tcgtcatcac gttgatcaag ccgcactacg aagcggcgcc
     1981 gccgctgctg cggaaaggga ttttgccgga agatcaaatc ccggcggtgt tggaatcggt
     2041 gaaggacgac gtgcagcatg cgggcttcac tgtcgcggcg atggtggaga gtccgatcaa
     2101 aggcgcgaag gggaacgtgg aaatgctggc gatgctaacc atgtagcacg ggcgtctcgc
     2161 ccgttccaag gtgcttcggc acgggcgaga cgcccgtgct acgagttgtt cgatttcgga
     2221 accaaccgct ggccgcgcgt ggcgcggatc ttcagggcac tcaaatatct ccgccgcagc
     2281 acgtcgcgaa gccgcgcgac ttccgtcggt gaaccattgg acagcgggat cacgccgccg
     2341 gctttggtgc ggtggccgcc gccttcgccc cagtttctga gcagcttgcg gatcatgtcg
     2401 gccgccgaca tggctttgct gctggtgcgc aggctcagga tcaaccggtt ctcgtgcact
     2461 gccgtcacca gcgcccattg cacctggtcg aagcgtagca agaagtccgc catcaccgcg
     2521 ggcttttcca gcgagtcgat ctcgtcgatg tgcgacatca acgcgttgtc gtaatacacc
     2581 gcgtggccca gcgccgacgc atacgcgatg taataactct gcggcaggtc aacgtaccgc
     2641 atctgataaa gccggtgcgt gtcggccatc agcgtcaggc tcgacagcgc gatgttgtcc
     2701 aattcccccg gtgttcccga tgcgccagcg aggtccgact cgatcgcgaa cagcagcgac
     2761 gccgccagcg ctggcgagat ctggacctcc agctccatga aatagctgaa gaccatcgag
     2821 ctggtcgcgc ccacttccgt gcggatgtcg cagaacgggc agtgcggcct ggtgtgcttt
     2881 ttcccctggt gatgatcgat caccacgtcg gtctcgatcg tcttcggcaa cgggctgaat
     2941 gaactctgcg gctgcgtatc gagcaagacg aacccgtcgt actccttcag ccgctcgtca
     3001 tcccacggca ccagttccag atcgctcagg ttcgcaaact tcgcattgat cccactggcg
     3061 accttgccct tgatcgacat gctgacgcgc gtaccggccg gcagcttggc gcgcagcaac
     3121 tggaccatcg ccatgctgct ggccagcgca tcggggtcgg gatgcagatg cgtggtgaca
     3181 aggatgttcc gcttgccggc caggacgcgc agcaggcgcg gcgcgcgcgg ccggccgcgc
     3241 gatttgcgct ccgccgccac cgccgagtcg gccacggttt ccaggcacga ctcgacgaag
     3301 ctggtcatgg gatgagtctg ttctttcacc gccgccaacg tcgcctcggc tactgcttta
     3361 gggttttgcc cgttggatcg tccagcggga gacacctgct tttcatcgac ttgcgacgtg
     3421 gtagccataa gagcgcgacc cggcggtccg ataacaggtg aatcatatgc gggtcaggtc
     3481 gggcgatgaa gggtgaaagt ctgcccggtc gggcaatcga cgagcgcgcg ctttcaaaag
     3541 cgagacattt cggtcgcgcg tgggacattt ttggcgaaat gtcccatgtt gtctcgcctg
     3601 cgcgcatcga gaaaaacgcc gtgggaattg ccggggaatt gcagcaactg tcgttcccgc
     3661 tcgcgtcgcg gtacgctcgc cggccttatg tcgcttgcga tcgacgtgga gacgggaaca
     3721 gatgaagtac gtaccgaccg gcacatcgtg ccggtcctcg tggccattgc aatcttcgcc
     3781 ggactgtcgc tctgggcggc aatcaagtcc gacggcttcc tcgaagccga cagttgcacg
     3841 cactacatct acgcgcgata tgccttcgcg cagccgcacc tgttcgtcaa catctggggg
     3901 cgaccggtct gcacggggat ttattcgatc ggcgcccact tcgccgggcg gatgggtgtg
     3961 cgcgtcacca gtctcattat cgccattgcg attgctctga tcgtcatgca gattgcgcgg
     4021 gggatgcaat ggggcgcgac ggcgccgacg ctggcgttga tcttcacgct cgcccagccg
     4081 ctggtgtttc tgcattcgtt cagcgaattg accgagctgc cgttcgcgct gctggtagcg
     4141 ctgggtttct gggcgtatca acgacaacgc tggctgtggt tcgcgctggc gatcggaatg
     4201 acaccgctga gccggcccga gggcttcggt ttgctcgcgc tggcggcggt ggcgctgctg
     4261 ctgcatcggc gttggtcgtg gctcgccctg ctcgcgctgc cgctggtggc gtgggattac
     4321 tgcggctggg tcgagtacgg ccgggtcggg ccttggtgga attggctccg ggcgaactgg
     4381 ccttacgagc agaagagcct gtacacgccg gggccgttgt accacttcct gttgctgatg
     4441 ccggtcgtga cgtcacccct gatcttcccg gcgatggtgg tgggtgcgtg gagatgtctg
     4501 acgccagatt tgcgcaaaaa gcgccaagac gccgctgacc ctgaacacct gcaaagatgc
     4561 aatgcgatca tcgccctgct gccgctgtcg atcctggtcg gtcacagcct gctctactgg
     4621 ctcggcaaga tggccagcaa cggtgagttg cgatacctgc tggtcgtggc acccttctgg
     4681 gcgttgctgg cggcgcgcgg gtggtcgtgg gtgttccagg aaactcgctg gcaacacgcg
     4741 gtcgcttggg ccgccgccgc ggcgctggcg ccggtgatga ttcatctgac gtacccggtg
     4801 ttgccgttgc accagcaacc ggattgggtg gaggcgcagc agatcgcgca gtggtatcag
     4861 gattcatcgc acgaccatca cccatacctg gcgacggcgc acccgggaat tttgtatcac
     4921 ctggacctga gcccgtcgga tggctcgcgc gtgcgcgagt ggcggaagga cgtgctcgac
     4981 gccgtgccgc cggggacgac ggttgtatgg gaccccatct atggcgtgtt caactccgac
     5041 aaatcgcgat cgatctcagc cgaagaattg ctaaacgcgg ggtggaagga agtgcagatg
     5101 ccgtggggcg ggtcagccgc ggcgggaaag tggcgcgtgt ttcaaagtca gcccattacg
     5161 gcgaaatcaa aatcaccctt accctaaccc tctcccggtg aacccgacgg cccgagactt
     5221 ttgcagactt agcaagggac cgacccaccc cgggttcgga ggtactccga cgaagcgaga
     5281 ttcttctgct cgacgcgcgg gaacaatccg ggtcgtcggc gtacctccga acccggcgtg
     5341 gtcggttcct aaccattcct gcaaaagtgt cggttcgtca gcccggtgta ccgggcgagg
     5401 ggaccagacg cgggcgccgc tggctcatgg cgtcgacaat gaacgcctcc atcgccagtc
     5461 gcgcatcgtg gtcgatcatc tgccgcggcg ggtgcttcat ggtccacgcg gcggcggcgt
     5521 tgatggggcc ggacatgccc gcatccagcg ccagcctgca gcagcgcacc gcatcgacga
     5581 cgacaccggc gctgttgggc gaatcctcca cgctcaatcg cagctccacg tgcatcggaa
     5641 ccccggcgaa acccgtgcct tcgatgcgaa ggaagcagac cttgttgtcc ttctggaacg
     5701 gcacgaaatc gctgggaccg atgtggatct gctcggagtc gagcggcaca tcgagctggc
     5761 tctgcaccgc ctcggtcttg ctgatccgtt tgctcgccag gcgatcgcgc gccagcatgt
     5821 tgagaaaatc cgtattccca gccgtgttga gttggtaggt cgagtcgatg cgcacaccgc
     5881 ggtcgtgaaa cagccgcgcg agcgtgcggt gagtgatcgt cgcgccgagt tgcgctttga
     5941 tgtcatcgcc gatgatcggc aggccgcgct cctggaatcg gcgcgcccac gcgggatcgg
     6001 acgcgacgaa gacggggatg cagttgacca gcgcgacgcc ggcgtcgagg caggcctgag
     6061 cgtaatgctc cgtcgcgcgc tgcgaaccga cggggagata gttgattagg atttccgcgc
     6121 cgctgtcgcg cagcacctcg gccacattga cggctggcag gctcgccggc tcgaaccgca
     6181 tgtgcggtgg gaactccagc atgtgcggtg gacagccgtc gagcgttggc cccttctgca
     6241 cgatcacgcc gcccgccggc agctcgggtt gaaacacctt cgtgttgttc ggtgccgcaa
     6301 agcacgccgc ttgcagtgac cggccgacct tgcgggcatc gatgtcgaac gcggcgacga
     6361 cgtccagatc gctcacgcga tatccgccca ggtcgggatg ctgcagcccc acatccctcg
     6421 gatgagcggc gtaaaaggca atgccctgga cgagcgaact ggcgcagttc cccacgccgg
     6481 cgatagcgat cttgatgcaa gacatagttg ggtgattcgc gggtcaaata atatagcgtt
     6541 gagggcgcga tttgttgccg gaggggataa gcaaccaaac cacgccgggt tcggaggtac
     6601 tccgacgacc cggatcttgc ggcgcgagaa cagaagaatc tcgcttcctc gcgggtacgc
     6661 tccgaacccg gcgtgtgaag acgcggttcc acgaacggtg gtaaaatata acagcggcga
     6721 cccccgccgc cgcgtaaact tctcagtgtc gggttggtcc tatggaactc cccgccggta
     6781 gaaccatcac ccgcggcgtg accgcgcgtg tggttcgaat attttccccg gtcggtggaa
     6841 cgatccagca aaggccgacc ggcggttccg ccgacgctgg tgagtagtac acgccggcga
     6901 acagatgagg cgggggacac acaccgggag gtgcgtcgtg aagcgattgt ttcgattgac
     6961 ggttttggca ctgttggttg gtggttgggc gctagcgggt tcggcgttgc acatcgtgcg
     7021 cacgccgggg cacgtgatcg tcctgccgaa gaatcggctg acgtttcatg atacgtacgt
     7081 tgacacccgc gcatggacga tccagaacgt gcgccagcat ccggacgtga cggcccgcat
     7141 cgtccaggtc ggcaaaggca acctcttgtc gcacgcggta gaaaacagca ccggcgacgt
     7201 gccggcgcag ctggcggacg ccatcgcgca ccccctggcc gccccgccga cgacgcgacc
     7261 ggcgatcgtt gacaaggcga tggccgaagt gcacgcggca acgcagtcgg tgcgcgcggt
     7321 ctttgattga cgacgctcgt cgaggttcgt tcaaattctt ctccttgata gccggggccg
     7381 gaaggcccgg tccgttgcac gttcaaacag accggggctt ccgcccccgg ctatcacgac
     7441 atcgttttcc cactgcccct gagcgatttt cacgttccat ccgtcccccg ggctcctgca
     7501 gattcttgac tcgatttccg cccgccccta tc
//
LOCUS       DATIMH010001213         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_2309949, whole genome shotgun sequence.
ACCESSION   DATIMH010001213 DATIMH010000000
VERSION     DATIMH010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309949"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..832
                     /locus_tag="VLI90_20970"
     CDS             152..832
                     /locus_tag="VLI90_20970"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HSV16751.1"
                     /translation="MTTSLRQILTLMATDLRVEARTRQTLGLVTVLGILIVVVLGLGL
                     GAEAAATRGFGATAVLWVAYLFSGVLCFEKTMAVERADGAMAALMVAPIDRGNIYLAK
                     LLSNLILMAGIAAVITPCGVLFFAFDLSIAPGTFVAVMSLAVIGFAAVGTLFAAVVSS
                     TRLQGGLLAMMIFPVTLPLVIASMQLLLHRFRDGQSIGGTGLGVLMAFDAMFLTLSWA
                     VFEWVLEP"
     gene            814..1557
                     /gene="ccsA"
                     /locus_tag="VLI90_20975"
     CDS             814..1557
                     /gene="ccsA"
                     /locus_tag="VLI90_20975"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSV16752.1"
                     /translation="MGPRTVMWQMLKTQSNVAAVYSTATVLAFGAAMGLLLTYTPTET
                     TMGPVQKIFYLHLPVAINTFVSCLLVFIASIGYLASQKTWWDDLAAAAAKTAVQLCAV
                     VLLTGMIWGRGAWGQWWTWSPRLTFSLALFLLYVVYLVVRSSIESRRQRAVIAAVYAI
                     IAFLDVPLVWLSARMMPDIHPGSIQLAPAMKLTLGVWFVPVTMLSLGLTIGRFSLIRR
                     SSDATHVKQSPAPASNSAASRFQIAGGVL"
     gene            1554..1703
                     /locus_tag="VLI90_20980"
     CDS             1554..1703
                     /locus_tag="VLI90_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSV16753.1"
                     /translation="MNGQQYLILAYSAGLGLLLSYVAGLWIAARLGRRHADKAGILQG
                     AGGVL"
     gene            1700..3013
                     /gene="hemA"
                     /locus_tag="VLI90_20985"
     CDS             1700..3013
                     /gene="hemA"
                     /locus_tag="VLI90_20985"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSV16754.1"
                     /translation="MSILLIGLNHRTAPVEVREQLAFSRDGVATALMLLRNQFPGAEA
                     SILSTCNRVEILIATENDRPTAQDLISFIAQARDLPANEFKNYLYQFSDEQAIRHLFR
                     VAGGLDSMVLGESQIVSQLKQAYAQAMEQGTTGRVLNRLFHHAFEVGKRVRTDTDIAE
                     GKLSVPSVAVDVARRIFADFSNKQTLVVGAGEMAQLVCQYLRDAECRKFTVTTRTLNN
                     ARALAEACDGQYVPFEKLDEQLAQADIVVTATACPMPILTLERVREIQKLRRGRHLFI
                     LDLAVPRNVAPEVATLEQVYVYDIDAIGRIVDENREHRGGQLAICERILDEEVAAFEQ
                     WLGQTKVNPVIAQMYMDATKVRDAEVRRMFNRCPDLTEAQQEEVLQLVDRLVGKFMHP
                     CVVSVRRHHNGQTWKTLAGALHEASDHIDEAPAGANGKRQKVRAS"
BASE COUNT          541 a          950 c          997 g          591 t
ORIGIN      
        1 cggggcggac ggtgctcgtc gtcgatcacg acatcgaacg cagcctgcgc atctgccagc
       61 gcatcatcgt cttgcgcggc ggtcgcgtgg cgctcgacaa agccgcgggt gacaccagtg
      121 ccgcggaaat cgaagcggaa atgagcggca catgaccacc tcgcttcgcc agattctaac
      181 cttgatggcc acagacctcc gcgtcgaggc ccgcacccgg cagacgcttg ggctggtcac
      241 cgtgctgggc atcctgatcg tcgtcgtgct cgggctgggc ttgggcgcgg aggcggcggc
      301 cacgcgcggc tttggcgcaa ccgccgtttt atgggtcgca tatctcttct ccggcgttct
      361 ttgctttgag aaaaccatgg ccgtggaacg tgccgacggc gcgatggcgg ccttgatggt
      421 ggcccccatc gaccggggaa acatttatct cgctaagctg ctgtcaaacc tgatcttaat
      481 ggccggaatc gccgcggtaa tcaccccctg cggggtgctg tttttcgcat ttgatttatc
      541 aatcgcgccc ggcacgtttg ttgccgttat gagtctggct gtgatcgggt ttgccgctgt
      601 cggaacgttg ttcgccgcgg tggtcagctc gacccggctg cagggcggtt tgctggcgat
      661 gatgatcttt ccggtgacgt tgccgctggt catcgcctcc atgcaactgc tgctgcaccg
      721 cttccgcgac gggcagtcga tcggaggcac gggcctgggt gtgttgatgg cgttcgacgc
      781 gatgttcctc acgctcagct gggcagtctt cgaatgggtc ctcgaaccgt gatgtggcaa
      841 atgttgaaga cgcaatcaaa tgtcgccgcc gtgtactcga ccgccacagt gctggcgttc
      901 ggcgcggcga tgggtttgct gctgacctat accccgactg aaaccaccat gggtcccgtg
      961 cagaagatct tctacctgca cctgccggtg gcgataaaca cgttcgtctc ctgcttgctc
     1021 gtcttcatcg ccagcatcgg ttacctggca agccagaaga cctggtggga tgacctggct
     1081 gccgcggcgg cgaagacggc ggtgcagctg tgcgcggtgg tgctgctgac cggcatgatc
     1141 tggggccgcg gggcgtgggg gcaatggtgg acctggtcgc cccggctgac attcagcctc
     1201 gcgctgttct tgctctacgt cgtctacctc gtcgttcgca gctcgatcga gtcgcgccgc
     1261 cagcgcgccg taatcgccgc ggtgtacgcg atcatcgctt ttctcgacgt gccgctggtc
     1321 tggctttcag cccgcatgat gccggacatc catccgggtt ccatccagct cgcgccggcg
     1381 atgaagctga cgctcggcgt gtggttcgtg ccggtgacga tgttgtcgct ggggctgaca
     1441 atcggccgct tctcgctcat ccgtcgttca agcgatgcaa cccacgtgaa acaatcgccg
     1501 gcaccggcga gcaattcggc agcatcgcga tttcaaatcg ccggaggtgt gttgtgaacg
     1561 gacagcaata tctgatcctc gcgtacagcg ccggcctcgg tttgctcttg agctacgtgg
     1621 cgggcctgtg gatcgccgct cgactcggcc gccggcacgc tgacaaagcc ggcatacttc
     1681 aaggggccgg aggtgtgctg tgagtatcct tctcattggc ttgaaccatc gcaccgcgcc
     1741 ggtggaggtc cgcgagcagc tggccttcag ccgcgacggt gtcgccaccg cgctgatgtt
     1801 gctccgcaac cagttccccg gcgctgaagc atcgatcctc agcacgtgca atcgcgtcga
     1861 gattttgatc gcgaccgaga acgatcgacc caccgctcaa gacctgatca gcttcattgc
     1921 ccaggcgcgc gatctgccgg ccaatgaatt caagaactac ctgtatcagt tcagcgacga
     1981 gcaggcgatc cgccacctct tccgcgtcgc cggcgggctt gactcgatgg tgctgggtga
     2041 atcgcagatc gtcagccaac tgaaacaggc ttatgcgcag gcgatggaac aggggacgac
     2101 gggccgggtg ttgaaccggc tgttccatca cgcgttcgag gtgggcaagc gcgtgcgaac
     2161 cgacaccgac attgccgagg gcaagctgtc ggtgccgtcg gtggcggtgg acgtcgcccg
     2221 gcggatcttc gcggatttca gcaacaagca aacgctcgtg gtcggcgccg gtgagatggc
     2281 gcagctggtg tgccagtacc tgcgcgacgc cgagtgccgc aagttcaccg tgacgacgcg
     2341 aacgctgaac aacgcccgcg cactggccga ggcgtgcgac gggcagtacg tgccattcga
     2401 gaagctggac gagcagctcg cgcaagcgga catcgtcgtc accgccacgg cttgcccgat
     2461 gccgatcctg acgctggagc gcgtccgcga aattcaaaaa ctccgccgcg gtcggcacct
     2521 gttcatcctt gacctggccg tgccgcgaaa cgtcgcgccg gaagtggcga cgctcgagca
     2581 ggtgtatgtg tatgacatcg acgccatcgg tcggatcgtg gacgaaaacc gcgaacatcg
     2641 cggtggacag ctggcgatct gcgagcgcat cctcgacgag gaagtcgccg cgttcgagca
     2701 atggctggga cagacgaagg tgaacccggt catcgcgcag atgtacatgg acgccacgaa
     2761 ggtgcgcgac gcggaagtgc ggcggatgtt caatcgctgc cccgacctga ccgaagcgca
     2821 gcaggaagaa gtgcttcagc tggtcgaccg gctggttggc aaattcatgc acccctgcgt
     2881 cgtcagcgtg cgccggcatc acaacggaca aacctggaaa actctggccg gcgcgctgca
     2941 cgaagcgagc gatcacatcg atgaagcgcc ggcgggcgcg aacggcaagc ggcagaaggt
     3001 gcgggcatca tgatcaagca atcgccgaca tccgtcctgc gacttggcac gcgcggcagc
     3061 cagctggcgc gcacgcaaa
//
LOCUS       DATIMH010001214         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tepidisphaeraceae bacterium isolate SMAG_U15765
            k141_4041528, whole genome shotgun sequence.
ACCESSION   DATIMH010001214 DATIMH010000000
VERSION     DATIMH010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936478
            Sequence Read Archive: SRR9650391
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidisphaeraceae bacterium (soil metagenome)
  ORGANISM  Tepidisphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Tepidisphaerales;
            Tepidisphaeraceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:24:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,163
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Tepidisphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4041528"
                     /isolate="SMAG_U15765"
                     /isolation_source="soil"
                     /db_xref="taxon:2876886"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37.89 N 123.48 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VLI90_20990"
     CDS             <1..1138
                     /locus_tag="VLI90_20990"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HSV16755.1"
                     /translation="LTWDAMDGLADEVALIFANNQPFEILMTKEGKTVSMPAGATAAD
                     VRAAHPYPNRRDPEGSTHHDAGTLWMGSDPATSVTNEFGRIHDTTNCYVAAPALFPSL
                     GSPNPMLTGVALSRRTADLLEGNVLPRPVIRNATAAGFTALFDGTVGSFKKWQAAGPS
                     AQGFTLTRGELVSYGAADFSLLYYAQQAFADFHLRLQFRVFDANNANSGVFVRFRNPL
                     ERMPTGLRQRADAEHAPVATNPAWSAVYSGFEVQIDDNARGDVNKDYYGRSPEPDGLY
                     KNRTGAIYKIPAGDLIVHTGGHDAALQLYSPGPAVRPGVWMQYDIVVTGDHYEVILTD
                     IDMGVGVVTTVFDNTDAQRGVAPGYIGIQSYPGSPVAFRDIWVK"
     gene            1208..2227
                     /locus_tag="VLI90_20995"
     CDS             1208..2227
                     /locus_tag="VLI90_20995"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HSV16756.1"
                     /translation="MNRVTRRLSLILLAIVTTLLTGTIGFTSIEHYPPFDAFYMTLTT
                     MTTVGYMEVHPLSTAGRVFNSFLIFFGVTTIFIAIGAMTQTIIELEFGDAISKRRNKR
                     MIDKLKDHFIICGFGRVGRGAAAELQHAGVPFVVVDVNPERVERAMLSGMLAANADST
                     RDDTLRLVGIDRARGLVAALATDADNLFVLLSAKGLNPRIFVAARAAEEGAEQKMRRA
                     GADAVFAPYAITGHRLAQSLLRPHVMQFLDFTTKDIGPDIAIEQIRVAEASQMASKSI
                     KDLQLGRDFGVIVMAIRTSDGEMHFNPTAESTVKPGDFLIVMGQQEKLRAVELLIAEA
                     RPTRR"
     gene            2467..>3478
                     /gene="thiC"
                     /locus_tag="VLI90_21000"
     CDS             2467..>3478
                     /gene="thiC"
                     /locus_tag="VLI90_21000"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase"
                     /protein_id="HSV16757.1"
                     /translation="MHYARQGKITEEMIYIAKRERIEPELVRSEVARGRMIIPANVNH
                     ANLEPMCIGVASKCKINSNIGNSAVTSDIEGELEKLAYSVKFGADTVMDLSTGGDIPA
                     IRKAIIGASPVPIGTVPIYEALSRVRRVEDLSKQVMLEVIEEQAEQGVDYMTIHAGVL
                     VQYIPLTTKRITGIVSRGGSILAEWMVKNHKQNMLYECFEDICKIFQKHDVSFSLGDG
                     LRPGSVADASDEAQFAELKTLGELTKKAWEYDVQVMIEGPGHIPMDEIEMQVQKEKEM
                     CYEAPFYTLGPLVTDIAPGYDHITSAIGAAMIGWYGASMLCYVTPKEHLGLPNRDDVK
                     AGI"
BASE COUNT          711 a          980 c         1172 g          615 t
ORIGIN      
        1 gctgacatgg gacgccatgg acggtctggc ggacgaagtc gccctgatct tcgcgaacaa
       61 tcagccgttc gagatcctca tgactaagga agggaagacg gttagcatgc cggccggcgc
      121 gaccgcggcg gacgttcgtg cggcgcatcc atatccgaac cggcgcgacc ccgaaggcag
      181 cacgcatcac gatgccggga cactctggat ggggtcggat ccagcgactt cggtgaccaa
      241 tgagttcggg cgcattcatg acaccaccaa ctgctacgtc gctgctcccg cgttgtttcc
      301 gagcctcggg tcgcccaatc cgatgctgac cggcgtggcg ctgtcgcggc gcaccgcgga
      361 cctgctcgag gggaatgtgc tgccgcggcc ggtgatccgc aatgcgacgg cggcgggatt
      421 tacggcattg ttcgacggca cggtcgggtc gtttaagaag tggcaggcag ccgggccgag
      481 cgcgcaggga ttcacgctca cgcgcggcga actggtcagc tatggggcgg ccgacttttc
      541 cctgctttac tatgcgcagc aggctttcgc cgatttccac ttgcggctgc aattccgggt
      601 attcgacgcg aacaacgcga atagcggcgt cttcgtgcgg ttccggaatc ctctggagcg
      661 catgccgaca gggttgcggc agcgcgcgga cgccgaacac gctccggtgg cgaccaaccc
      721 cgcctggagc gccgtgtact cgggtttcga ggtgcagatc gacgacaatg cccggggcga
      781 tgtgaacaag gactactacg gccggagtcc ggaacccgac gggctataca agaatcgcac
      841 gggcgcgatc tacaagattc cggcgggcga cctgattgtg cacacgggcg ggcatgatgc
      901 cgcgctgcag ttgtacagtc cgggtccggc ggtccggccg ggcgtgtgga tgcagtacga
      961 catcgtggtc actggcgatc actacgaagt cattttgacc gacatagata tgggcgtggg
     1021 ggtcgtcacg accgtcttcg acaacaccga cgcgcaacgc ggcgttgcgc cgggatatat
     1081 cgggattcag tcctaccccg gttctccggt tgcgttccgt gacatctggg tgaagtagag
     1141 cgtttgcacg ggggcagatg gccacgcatc tgccccacga agtagcaggc gttatacttg
     1201 tcgttcgatg aaccgtgtga cgcgccgact ctccctgatc ctcctggcga tcgtcaccac
     1261 gctgctgacc ggcaccatcg gattcacctc gatcgaacac tatccgccgt tcgacgcgtt
     1321 ctacatgacc ctgaccacga tgacgacggt cgggtacatg gaggtgcatc cgctgtcgac
     1381 ggcggggcgc gtgttcaatt ccttcctgat tttcttcggc gtaacgacca tcttcatcgc
     1441 gatcggcgcc atgacccaga ccatcattga actggagttc ggggacgcca tcagcaaacg
     1501 ccggaataaa cgtatgatcg acaagctcaa ggaccacttc attatttgcg gcttcgggcg
     1561 cgtcgggcgc ggcgcggcgg cggagcttca gcacgcggga gtcccgttcg tcgtggtgga
     1621 cgtcaatccg gagcgagtcg agcgggcgat gctgtccggg atgctggcgg ccaacgcgga
     1681 ttcgacgcgt gacgacacgc tgcggctggt cggtatcgat cgggcgcgcg ggctggtggc
     1741 ggcgctggcg acggacgcgg acaatctgtt cgtgctgctg tccgccaaag gtctgaatcc
     1801 gcggattttc gtggcggcgc gggcggcgga agaaggcgcc gagcagaaga tgcggcgggc
     1861 gggggctgat gccgtgttcg cgccctacgc gatcaccggc caccggctgg cgcaatctct
     1921 gctgcggccg cacgtgatgc aattcctgga tttcacgacg aaggatatcg ggccggacat
     1981 cgcgatcgag cagatccggg tcgcggaggc gagtcagatg gcctcgaaga gcatcaagga
     2041 cctgcaattg ggtcgtgatt tcggcgtgat cgtgatggcg atccggacca gtgatggcga
     2101 gatgcacttc aatcccacgg cggagtcgac cgtgaagccg ggcgatttcc tgatcgtcat
     2161 gggacagcag gagaagttgc gggcggtgga actcctgatc gcggaggcgc ggccgacgcg
     2221 cagataaata cgaggtcgtc cgcgcgtaag cggttataat cgcatacgta agggaggatc
     2281 ttgtggccaa ggacagaagc ccgaaaaaag agacgaagaa gccgaagaaa aaaaagtagg
     2341 ttcccccagg taacgtagac gggccgggct ttccggttgg gaccagagtc cggccgctac
     2401 actcaggagt cagtcatgcg cacagagtgg gtagcaaaac ggaaaaacga cagtaatcgc
     2461 acgcagatgc actatgcccg gcagggcaaa attacggagg agatgatcta catcgcgaag
     2521 cgcgagagga tcgagccgga actggtccgc tccgaagtgg cgcgcgggcg aatgattatc
     2581 ccggccaatg tgaatcacgc gaacctcgag cccatgtgca tcggggtggc gtcgaagtgc
     2641 aagatcaact ccaatatcgg caactcggca gtgacgtcgg acatcgaagg tgagctcgag
     2701 aagctggcct actccgtgaa gtttggcgcc gacacggtga tggatctttc gacgggcgga
     2761 gatatccctg cgatccggaa agcgattatc ggggcatcgc cggtgccgat cggcacggtt
     2821 ccgatctacg aagcactctc gcgggtgcgg cgggtggagg acctcagcaa gcaggtgatg
     2881 ctggaagtca tcgaagagca ggcggagcag ggcgtcgatt acatgacgat ccatgcgggc
     2941 gtgctggtcc aatacatccc gctgaccacg aagcgcatca cgggaatcgt cagccgcggc
     3001 gggtcgatcc tggccgagtg gatggtgaag aaccacaagc agaacatgct gtacgagtgc
     3061 ttcgaggaca tttgcaagat cttccagaag cacgacgtga gcttctcgct cggcgacgga
     3121 cttcgtcccg gcagtgtggc ggacgccagc gacgaggcgc aattcgccga attgaagacg
     3181 ctcggcgaac tgaccaagaa ggcctgggaa tacgacgtcc aggtgatgat cgaaggtccc
     3241 gggcatattc cgatggacga gatcgagatg caggtccaga aggaaaagga gatgtgctac
     3301 gaggcgccct tctatactct ggggccgctg gtgacggaca tcgcgccggg ttacgaccac
     3361 atcacgtcgg cgatcggcgc ggccatgatc gggtggtacg gcgcgtcgat gctctgctac
     3421 gtgacgccga aggagcacct gggactgccg aatcgcgacg acgtgaaggc agggatta
//
